LOCUS       DAMJVK010000001       111180 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.001, whole genome shotgun sequence.
ACCESSION   DAMJVK010000001 DAMJVK010000000
VERSION     DAMJVK010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 111180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 111180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..111180
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.001"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            161..1354
                     /locus_tag="PC414_000001"
     CDS             161..1354
                     /locus_tag="PC414_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HDF2462424.1"
                     /translation="MSKVFEVKKDIYFTGVVDEGLKVFDIIMETEFGTTYNSYLIKDE
                     KTVLFDTVKANFKDEFLSNLSEVTDIAKIDYVVIHHTEPDHAGSLKYLLDINPNIEVY
                     CTKAAKLYLDGQINRPFNCHVIKDGEILNIGKRNLRFITAPFLHWVDTMFTYIEEDKT
                     LLTCDAFGCHFASVDAEVVNSEDYLKSAKHYYDCIVKPFAKHVLSAVDKVVGLNIEFD
                     TILTSHGPMLTKDPMAAVKRYVEWSTEAVNTTNQNQVSIFYLSAYSNTLEMAKKIKEG
                     LDKEGAKAELYDLEDMTLTEMHDTLVVSKVILLGSPTINKTMVKPMWDLFSVIDPMAN
                     QGKIAGVFGSFGWSGEGITMAETLLKSMSFKMPVESLKKKFFPSEETLKECMAFGAEF
                     AKLVK"
     gene            complement(1566..2386)
                     /locus_tag="PC414_000002"
                     /pseudo
     CDS             complement(1566..2386)
                     /locus_tag="PC414_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428476.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(2516..3307)
                     /locus_tag="PC414_000003"
     CDS             complement(2516..3307)
                     /locus_tag="PC414_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2462425.1"
                     /translation="MKLIDEYLDKLYKKCDNKSTIELKQEMRCHLIESANEFKLEGLD
                     EEEACKKAIERFDDGDEMQYELCNIIKELSLSLDRHKSIVMGFKKVLGYISIIAFLIS
                     GFMWYYNNSLQHNMYNLGKELDGEIKQLAERHDMTKIGEYKLELEKILDKDKYSKVKA
                     LRLYVIDMKDGNTNLSSSGLNANMVYEREADYNNISNFIQHLGYNGKDFLDKNGNIVN
                     PDIFLEYFFYFESEMLIPVAFVFGLLCIIAYFILRFKISLIKNNN"
     gene            complement(3311..3640)
                     /locus_tag="PC414_000004"
     CDS             complement(3311..3640)
                     /locus_tag="PC414_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2462426.1"
                     /translation="MQLNKEVLKGYIDILIVSILEKKDCYGYEIAKQVRERSEFELKE
                     GTMYLALKRMESKNLIKSYYSNEQSSGGRRKYYNLTNEGKDFLEIKKQEWRFIKKVMN
                     QFLGEVD"
     gene            complement(4125..4538)
                     /locus_tag="PC414_000005"
     CDS             complement(4125..4538)
                     /locus_tag="PC414_000005"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HDF2462427.1"
                     /translation="MNIALVAHDQMKNTMVGFCIGYESILKKYGLYATGTTGKRIMDE
                     TELNINRLASGPLGGDQQIGSLIVTQEIDLVIFLRDPLTSQAHETDIQALIRLCDVYH
                     VPIATNLASAEIFIKALDRGELSWREVRKSKSQRI"
     gene            complement(4579..5709)
                     /gene="rodA"
                     /locus_tag="PC414_000006"
     CDS             complement(4579..5709)
                     /gene="rodA"
                     /locus_tag="PC414_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HDF2462428.1"
                     /translation="MKKININYKSTIKLIKQLDWKLIVTVLAIFIFGLVILSSATHAN
                     STGSYNQLIKQGLAFVLGIGMIIVILFFDYNLLGRYYKALYIISLILLAIVLLPGIGT
                     VKGGARSWINLGPLDLQTSEIVKLTFVLSYAKILESKKDKLNTLKEVMPVVVYSLPFI
                     GLLIAQPDLGTGIVFCCMIFAMLFTAGLSSKLIKRGIIILLVSMPLMYLMMADHQKVR
                     IEAFLNPEDVTLKGNYQVMQSLIAIGSGGVTGKGLYNGSQNQEDFLPVQDSDFIFAVV
                     GEELGVIGMAVLIILFMIFLLRLLAIARDAKDFYGTLIVVGVMGMFGYQIIQNIGMTV
                     ALIPVTGVTLPFVSYGGSSLLTSLANLGLVLNVCMRRKKINF"
     gene            complement(5794..6078)
                     /gene="minE"
                     /locus_tag="PC414_000007"
     CDS             complement(5794..6078)
                     /gene="minE"
                     /locus_tag="PC414_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HDF2462429.1"
                     /translation="MLDLFRVFSNEAKTSKSVAKERLKLVLVHDRVDCSPQLLEMIKT
                     DILKVIANYAEIEDDGLEIKMSKCRGEHDDKPVSALVANIPLKNIKDRCM"
     gene            complement(6096..6893)
                     /gene="minD"
                     /locus_tag="PC414_000008"
     CDS             complement(6096..6893)
                     /gene="minD"
                     /locus_tag="PC414_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HDF2462430.1"
                     /translation="MSEVIVITSGKGGVGKTTTAANLGTALSLENKKTVVVDADIGLR
                     NLDVVMGLENRIVYDIVDVVEGTCRLKQALIKDKRFDNLYLLPAAQTRDKNAVSVEQM
                     IDLCEKLKESFEYIIIDCPAGIEQGFKNAVAGADRAIVVTNPEISAVRDADRIIGLLE
                     ANEIKEIRLVINRIRNDMVKRGDMMDKQDIIEILAIDLLGLVPDDESIIISTNKGEPA
                     ILDSKSLAGQAYKNIAKRILNEEVPLLDLEVEDGFFGRLKKMFSMAK"
     gene            complement(6917..7600)
                     /locus_tag="PC414_000009"
     CDS             complement(6917..7600)
                     /locus_tag="PC414_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HDF2462431.1"
                     /translation="MSLREICSQELVEFKGNKRGIIVNIKREAPFEEIQEKIINKLEA
                     YVGFFNGAKISKINSDCLTDMEILELKEGITSRFDVEFVEDQKIEENSNFPTKYVNTL
                     RSGENIEFEGDVVILNDMKPGSKVLSKSNTVVMGDINAGAKVVAGGNVFVMGKIEGFV
                     HAGAEGNEFAYVVAGNLNPKILQIADNIAEAPDDEENYESESEISPEIAFVSNGRIVI
                     ESYLSKLDK"
     gene            complement(7698..10676)
                     /locus_tag="PC414_000010"
     CDS             complement(7698..10676)
                     /locus_tag="PC414_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDF2462432.1"
                     /translation="MEENKQVDRLKIIKIVMGVIFLAILVKIIYMTTFKYEYYNELAE
                     NKTYKKLAIEAPRGEIKDRYGRLLAGNKNLFTVQVSGNDINKKDANKHSRANEISLKL
                     INLLERNGEEYVDEFPIYVENGKYYYTYDRDIREYKSENGIPNDYNAKESFYYLVDKL
                     ISAGILSQEDKRLDATRLQAKLNENGYYPPILVSKWMFTAERDKRDWLASYKIKETKL
                     SAKEAFEKVRNSDALEIDKSLSDEDARKIMVVRDLIKSKGYSQYNPVTIAKDVGETTI
                     AQIEESAMDLVGVSIAVEPVRYYPNGSLASHMLGYVGKMPSTQIESYLQKGYETGDMV
                     GLAGVEKSNESRLRGTDGYKMVKVDALGRISKEIESKKPKSGDTVYLTLDKDLQEVSD
                     NALKQIIEVASKGGTFKSKFGDKPISAYAGKAQSAALIAIDVKNGEVLASSSYPNYDP
                     NKFAKGISTEDYKALQPKNPNDLLAGSPLLNLVTQGEFQPGSSFKMVTSMAALENGLD
                     PNFTINDPGVIMLGKKSFGDYVWNHGRGNHGMTNLYKAIQESCNIYMATIGTGKTWPD
                     GKSIGIDMNANKILEYAKLFGLDQNTGLQDEVEERAGKVPSTEDKLKSTQALLKSNLE
                     REMANDFVDITREKNPKEYEKRINEIVSWAAEKKTPGRVETMNRLKKLDVKEDRIEEV
                     ADLAVFSYFNFAKWSTADTFNLAIGQGENAYTPAQISRYVAAIANGGNLVELSVVDRA
                     VSSDYSSVKINDQKKVEKIPFKNPDNLKELTKGMKLVARQGTAKSAFADFPIDVAAKT
                     GTAEKSGKIPTDNEYEYLKSHMSSYNVNLNDAIKLADKMKAEKEKELSLAKEKEIKKK
                     LENKDLKDEERKKLEEELEDGVKVRLEDTDKVNSSYLRKAIKELNPKITDDQIDRFKQ
                     DYGSFTWTVAFAPADDPEIAVVCVIPQGDSSVFSLLPTREVIGTYMGLEPTNSKNDNK
                     TNDVNNSSDENINFESQINR"
     gene            complement(10688..11176)
                     /gene="mreD"
                     /locus_tag="PC414_000011"
     CDS             complement(10688..11176)
                     /gene="mreD"
                     /locus_tag="PC414_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HDF2462433.1"
                     /translation="MKKVLLCLLGIVLMTIENSITNYIDIFGISFNLVLIYVTIISLY
                     LDELEGGIIAAIIGLIKDITVGGIFGVNALILFIIAYAIGYMRDQLYKGSYIITFVLI
                     FIGSLIDSIVNIGTSSIIYQSYNISTLFVKGLLVAPIVNSLIGLVIYRLSKRAVLKLK
                     ED"
     gene            complement(11191..12084)
                     /gene="mreC"
                     /locus_tag="PC414_000012"
     CDS             complement(11191..12084)
                     /gene="mreC"
                     /locus_tag="PC414_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDF2462434.1"
                     /translation="MALRFDKNKNEKKRINVKVIATGVVAITLIGIVGISIGKFSSGS
                     PVNLGVASDAITSVGKGINDGFSFIKNGFKDVANFKDNSKKVKKLEEENEKLKKNMIA
                     LNAKLDKTESLEELKKTLNFVQEEYKATSISASVVGKNDGNWYESFVIGAGKNSGVKK
                     ESIVMNGSGLVGIVYEVSNNYSKAISLLDSKASVSFKLAKDANAKGTITQNTTLDNKD
                     SYNSKGYLQGYMFDSSYNVIQGDIITTSGLGFFPDGIPIGEVEKVVDDKDKSLKYVVV
                     KPYVDFKNINDVVVIEPRNIG"
     gene            complement(12094..13104)
                     /locus_tag="PC414_000013"
     CDS             complement(12094..13104)
                     /locus_tag="PC414_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HDF2462435.1"
                     /translation="MTKDMGIDLGTANTLVYIKGQGIVVREPSVVAIRDDSKEVLAVG
                     EEAKKMIGRTPGNIVAIRPMKDGVIADFDITQSMISYFIQKAADKKGVVSPRIAICVP
                     FGVTEVEKRAIEEAARQAGAKDAFLIEEPMAAAIGAGLKVEEPEGNMVVDIGGGTSEI
                     AVISLGGIVTAKSIRIGGDEFDESIVAYVKKEYNLMIGERTAENVKINIGSTFKDDEE
                     INMQIRGRDLISGLPKTIEICSTEVREALKEPVSSIVDAIKSTLERTPPELASDIMEN
                     GIMLTGGGALLRGLDKLITQETGMSVQIAETPLDCVALGTGKSVEDQEIFEKVLMMNT
                     KN"
     gene            complement(13225..13884)
                     /gene="radC"
                     /locus_tag="PC414_000014"
     CDS             complement(13225..13884)
                     /gene="radC"
                     /locus_tag="PC414_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HDF2462436.1"
                     /translation="MAPEERPREKMLAKGVKSLSNAELLAILLRTGNKNKNAIELANY
                     IINRDIQGIRHLEDMTIEELCNIDGIGLSKSTQIKAALELGSRVASFKPIKYKIRNPW
                     DIQRYYMDSLRYLKKEVFKAVLLNTKNEIISDVDVSIGTLSSSLVHPREVFKEAIRRS
                     ASKIIVMHNHPSGSVEPSREDKNITSRLIKCGEIIGIEIIDHIIIGDGLYFSFKENMI
                     I"
     gene            complement(13929..14513)
                     /gene="maf"
                     /locus_tag="PC414_000015"
     CDS             complement(13929..14513)
                     /gene="maf"
                     /locus_tag="PC414_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HDF2462437.1"
                     /translation="MNIILASASPRRKEILENTNVRFDIIKNEIDEIILEGETPKHLV
                     MRLAFEKSMSVASEHNEDVVIGADTVVVLDNAILGKPKDESCARDMLKRLSGREHQVI
                     TGISLINLCEDKKVIDYVISNVKFKTLSEQDIEDYLKTNESFDKAGAYGIQGYGALLV
                     EEIRGDYFNIVGLPISRLGDLLKKYFSINLFYGV"
     gene            complement(14733..15704)
                     /locus_tag="PC414_000016"
     CDS             complement(14733..15704)
                     /locus_tag="PC414_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HDF2462438.1"
                     /translation="MVILTAVLVLGIMGLIFGIVLDFASKKFAVEVDERVEAILGVLP
                     GANCGGCGFPGCGGLANAIVEGNAPVNGCPVGGADVGAKVGEIMGISAEAGEKQVAKV
                     ICKGTCSSAKDKYEYEGISDCRAANVLNSGAKMCKFGCLGLGTCKDACKFDAISIVDG
                     IAVIDEEKCVNCGKCKEVCPKGIIITKPESQEVVVECNSKEFGKAVKEKCTAGCIGCG
                     MCVKACKFDAIIFEDKIAKIDPNKCVGCMQCVAKCPTKVISGDITKKKKVTIDQELCV
                     GCTVCKKQCKFDAIEGELKEKHKVDADKCVGCHLCMEKCPKKAIKIL"
     gene            complement(15720..16295)
                     /gene="rsxA"
                     /locus_tag="PC414_000017"
     CDS             complement(15720..16295)
                     /gene="rsxA"
                     /locus_tag="PC414_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HDF2462439.1"
                     /translation="MNLILLFLSIVLVNNVITSQFLGICPFLGVSKKVDTAVGMGVAV
                     TFVLTLASIITYFIQILLVKTGTGFLQTIAFILVIASIVQFVEMVIQKMSPSLYQALG
                     VYLPLITTNCAVLGIALVNVQKSYNLVETIINGFGAGAGFTLAIVIFAGIRERLELAD
                     IPEAFKGFPITLISAGLMSIAFLGFTGLIKL"
     gene            complement(16310..16903)
                     /locus_tag="PC414_000018"
     CDS             complement(16310..16903)
                     /locus_tag="PC414_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HDF2462440.1"
                     /translation="MNLAKVFKNGLIDENPTFVQVIGMCPTLAVTTSAINGIGMGLST
                     AAVLICANLVISLIRKITPDKIRIPIFIVVIATFVTIVGMLLKAYVPALDKALGIYIP
                     LIVVNCLILARAESFAFKTGAMPSIVDGVGQGLGFTVALTIIGAVRELLGNGSLFGMT
                     LFGASFQPVLIFILPPGAFLTLGFLFAGFNKLRSKKA"
     gene            complement(16916..17485)
                     /locus_tag="PC414_000019"
     CDS             complement(16916..17485)
                     /locus_tag="PC414_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HDF2462441.1"
                     /translation="MNSMVRLGGTLLAISAIAALALGATNQVTAPVIEQRNIQANNEL
                     RKAVLPEAKEFKEMKEDTYKGLGDGLIAEVYEGLDGSEVVGYTLKAKPSGYGGEIEVM
                     VGISSEGQVTGVDIGNMSETAGLGAKAKDDAFKGQYKGKTAEPLEVAKGSTTADNQIL
                     AISGATITSTAVTTGVNAAIDVFNSALNK"
     gene            complement(17485..18462)
                     /locus_tag="PC414_000020"
     CDS             complement(17485..18462)
                     /locus_tag="PC414_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HDF2462442.1"
                     /translation="MENKLIVSSSPHVRSNEDTSYIMKQVIIALLPAAVAGVYFFRLN
                     ALSAMFFCILGTVGTEFLYQKFTKQKSTIGDFSAVVTGLLLAFNVPASLPWWMCLVGG
                     IFAILVVKMVFGGIGCNFVNPALAARAFLLASFPVAMTAWTQPGVNWIGKNLDAVTTA
                     TPLSFLKNGAAGLADLSSNGISLADMMIGNIGGCIGETSAILLLLGGVYLMYKGIINY
                     VIPVFYIATVFILTFLLGGFNITFAIYQLFAGGLMLGGFFMLTDYTTSPMTKKGQIIY
                     AVLAGLITTVIRMYGGYPEGVSYSILLVNCLAPLIDKFVRNRVFGEVAK"
     gene            complement(18485..19822)
                     /gene="rsxC"
                     /locus_tag="PC414_000021"
     CDS             complement(18485..19822)
                     /gene="rsxC"
                     /locus_tag="PC414_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HDF2462443.1"
                     /translation="MKLLTFKGGIHPPYRKEYSNTKALEKAQAPKIVYIPLQQHIGAP
                     AKPIVEVGDEVKFGQKIGEQQGFVSCNVHSSVSGKVIAIEQHEVPGGSAQCVVIENDF
                     KEELHESVQPKGQLEDLSKEDIVGIIKEAGIVGMGGATFPNHVKVSPPPDSKAEVVIL
                     NGAECEPYLTADHRLMVENPEDVVFGLRALMKVLDVKKGFIGIETNKPDAIEAIQNVA
                     KDYSEIEVVGLQVKYPQGAEKQLIYACTGKEVPSGGLPIAAGAVVDNVATAAQIAKSI
                     KTGMPLVERITTITGSCIKEPKNLITKVGTLVSEIIEQCGGFKEDKKIGKVIMGGPMM
                     GIAQYTTEIATNKGSSGILCLDEEESRTPDIQNCLRCGRCTDVCPSFLQPLFISAYSL
                     KDDYDTAEYHRAMDCIECGSCSFICPARRPLLQSIRSAKREIGAKRRKQAAQK"
     gene            complement(20038..20982)
                     /locus_tag="PC414_000022"
     CDS             complement(20038..20982)
                     /locus_tag="PC414_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2462444.1"
                     /translation="MNRRKTIYRGFNNNRHKIDLRKYVITIACLCLIGYYSYTKIKDS
                     KILEYVSAKIPFLNNSSDITYKDISDELNSIKKGKKSKSQTNSDDKQETNPEKAVNNT
                     KEPEEVKLATIEGWDMYTIQVAAIDNNDDLKKIQTSLVNNDIPFSVMEKDGVKKIQTY
                     SSFDENDVRKQISSVRKVFPDAFLSHLDAPMLSLEYTSNYAYIESISKELNKLITNFK
                     EESSFWSNAENNVDMEKYNTILTNRKAISQNISKEAEKIDYSEMRLFKDNLIEYVKNV
                     NEKIDTASKAANEEKYSVSKSLLLSSMQEYSMFINSIK"
     gene            complement(21093..22367)
                     /locus_tag="PC414_000023"
     CDS             complement(21093..22367)
                     /locus_tag="PC414_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDF2462445.1"
                     /translation="MKKAIAALGIGAVAVSVSSINASALEKGTVTASALNIRSGPSSD
                     CDKVAKLYKGKTVEILEKSNGWYKVRVSSSVVGWGSAKYISTSGSSEGTSSQNNPTSS
                     GTTISGNGKVNVSSRLNVRSGAGTNYSLVGKANNGDVVKLLEQSNGWYKIKLSNGVTG
                     WASSQYISKTSEDVGTNNSSNSNSTNNSDKKPSSEESIEGKNGKVTSAVSLNVRSGPG
                     TSYSIIGKLNGGDVVELKAKSNGWYKVKLSSGTIGWVSASYISETNEDTKEKPNSSSN
                     QNSQSNSNSKPSFTGNSDKSTAKGSTIVDFAYTLIGIPYQWGASGPDKFDCSGFTQYV
                     FKHSVGVSIPRVSREQANFGSAISMGNYAPGDLVYFDTDGDGTTNHVGIYVGNSKFIH
                     CSGTQTNPNKVKVDNLTSSYWSKVLLGARRFV"
     gene            complement(22499..22870)
                     /locus_tag="PC414_000024"
     CDS             complement(22499..22870)
                     /locus_tag="PC414_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF2462446.1"
                     /translation="MKFNFCHNNFNVTNLEKSLDFYKKALGLKEVKRKEAEDGSFILV
                     YLGDGITSHTLELTWLRDWDRPYNLGDNEFHLALEVEEFDEAKKLHKDLDCICFENES
                     MGIYFIADPDNYWIEILPKNH"
     gene            complement(23098..23697)
                     /locus_tag="PC414_000025"
     CDS             complement(23098..23697)
                     /locus_tag="PC414_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2462447.1"
                     /translation="MESRGYKEDLKKMILDMAHKELESYVSQKGAKKYFEGNLDKIIN
                     NNIKKFNQSIGLNRVYLKMLKKCAYQESVTSISRTIKKEALFKSKKELFAFSEYLKLD
                     INKRLSYNQILRKISRYIYYNRSSYAQKYVVFKRGDEEYLLEPEKIKDELISSYRSKT
                     REDMRSIAKLLDIEVEDDYNAEDIRKKVINYIIKEKLKS"
     gene            complement(23898..24110)
                     /locus_tag="PC414_000026"
     CDS             complement(23898..24110)
                     /locus_tag="PC414_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HDF2462448.1"
                     /translation="MKKKLLVEGMSCGHCVNHLKTALTEDIDGIEVLDVDLENKCASV
                     DMKDDVSIEKLKEVIAELGFELKGIE"
     gene            complement(24183..25898)
                     /locus_tag="PC414_000027"
     CDS             complement(24183..25898)
                     /locus_tag="PC414_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2462449.1"
                     /translation="MKRIKVLTVVLAITLMVAGCSNTKTKQSSSDSSLQSQSSIDAKY
                     INLTMVTPKTINPITNTDKSVGYIMNLVYDSLFTIDENYNVIPQLVKEYNIAQDGMSI
                     DIKLKDAKWHDGKNVTSNDVNYTIGLIQKSADSPYNEFTKNIASVNIKSDKDFTIKFK
                     ARYAFSIDSLIFPIVSQNHLDSKDVNDKNNNMIGNGKYKIESYTEREGMVLSVNKDYY
                     EEVPKTMKNIKVGMVPNEDARTSMVMALDSDITNVTLNDLSKFQEKEFNITKYQGRDY
                     ECVLFNYNNPFFKDVNFRKAIAHSIDKDRIISEGYMDDATPVNFPLNSKSKYYNSEMK
                     DLEFNKDKAIECLAKVEYANVNSVNQNDNKVKNQKKSAKKNSKKLTPEEEAKVKKAEE
                     DRIKKEAEEKKNREKEEVKKSLSEMNLKIIVNRDNSERIKTANIINENLKAIGINSTV
                     NQLSDKDMENALNSKNYDLALVGWKLSIIPDASSIIASSGYTDDKLNGYMSALTSSTS
                     EIETKKAYKDVQTYIKDNATFISLAVRNNYIVSNSRLKGKITPNDFDVYEGISNLDIK
                     SNESN"
     gene            complement(25898..26452)
                     /locus_tag="PC414_000028"
     CDS             complement(25898..26452)
                     /locus_tag="PC414_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HDF2462450.1"
                     /translation="MNSKKVLILSIFIILFGALLMESKVIHKFLYPKKYSEYVEKYSK
                     EFNLDENIVYSVIKAESKFNSSAVSKKEAKGLMQILDITRDWGAEELNLKNVDIFDPE
                     TNIRLGCWYLSKLYKEFGKLDLVIAAYNGGSGNVKKWLENNEYSKDGENLHDIPFKQT
                     SKYVEKVKNNYEHYNKIYGKKGKN"
     gene            complement(26445..27047)
                     /locus_tag="PC414_000029"
     CDS             complement(26445..27047)
                     /locus_tag="PC414_000029"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HDF2462451.1"
                     /translation="MLILGLTGGIGCGKSSLSNIFRNLNIPIVDADIISRKIFEDKLL
                     LEKVFVHFGQSIKNDDGTLNRKALGKIVFSDEEKLKELNNLTHPRIREKIISEIEKLR
                     KKGENIVVLDAAILVESGFLDMVDKLLVVTCKQEVQISRIQKRDNCSEQEALSRINSQ
                     MSQEEKSKYGDYIIDNSGTITELESKAHKFIEYMKENWRE"
     gene            complement(27056..29704)
                     /gene="polA"
                     /locus_tag="PC414_000030"
     CDS             complement(27056..29704)
                     /gene="polA"
                     /locus_tag="PC414_000030"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDF2462452.1"
                     /translation="MEKKLIIIDGNSIINRAFYALPEMNNKEGLKTNAIYGFTTMLFK
                     MIDIYKPTHISVAFDRKAPTFRHLEYKEYKAGRKGMPDELAEQLQPLKDLLDKFRINR
                     LEIDGYEADDIIGTVSKKAEENGYKVYIVTGDKDAIQLASDNTTTLITKKGVGEVEEY
                     NFNSVIEKYEMTPTQFIDLKGLMGDKSDNIPGVPGIGEKTGIKLIKEFSSIENLIENT
                     DKLKGSVKKKIEENKEIAIQSKRLATIIRDVPIEVDLDSMIFGDYDKDELLDKFRYFG
                     FTSLLSRLVDLVDSDDTEHVEEKIEILKLKDIEKFIDEVNKKGQVILKTVRGQGNILE
                     KNIIYIFISVDGEKIYYVDSNEVSKLDSIFSNNEIKKYGYNLKDDYISLKPYNINLEN
                     MYFDITIAEYLIDSTSSTSYDCSAIAMKYLSKKVKSKEELLGKGVKSKNYEDLEFEDL
                     AEYMGQIICTVKETVPMMERSLMDMSMDGLFYHVEMPLVEVLGHMEYEGIKVDKSILD
                     ELSVEFKEIIATLEKEIYELSGEPFNINSPKQLGVILFEKLELPIIKKTKTGYSTNAD
                     VLEKLRDKHPIIDKITEYRQIVKLNSTYVEGLLNIINPESNRIHSSFNQTITTTGRIS
                     STEPNLQNIPIKMEMGRKIRKVFIADDNCKLVDADYSQVELRVLAHMSQDENMIEAFK
                     HHEDIHTKTASQVFNVPMEDVTSELRGAAKAVNFGIIYGKSDFGLADDLNIPVPKAKE
                     YIESYFAKYHKIKEFMDTTVEKASEDGYAVTILNRRRYIPEIKSSNFMERNRGKRFAM
                     NAPIQGSAADIIKIAMINVHKKLEENNLKSKLILQVHDELIVEAIDDEIDIVKKIVKE
                     EMENAVNMDVHLDVDLNVGSSWYETK"
     gene            29856..29943
                     /locus_tag="PC414_000031"
     tRNA            29856..29943
                     /locus_tag="PC414_000031"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29890..29892,aa:Leu,seq:caa)
     gene            complement(30130..30573)
                     /locus_tag="PC414_000032"
     CDS             complement(30130..30573)
                     /locus_tag="PC414_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2462453.1"
                     /translation="MKKNMLYVGIIYFICGILLVLLATFTEFSFEAFIWGLSGAALGP
                     GVCMILQYMYWSKPERAVDYEEKIKNQRIEMNDERRIMLRDKAGRITNQIMSYVLVIL
                     IFIVSILSVFSVMVISKWVLVVLGLLILFQFICSVVVYNKLDKKL"
     gene            complement(30576..30773)
                     /locus_tag="PC414_000033"
     CDS             complement(30576..30773)
                     /locus_tag="PC414_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2462454.1"
                     /translation="MKNRLEEIRKERGIKQEELASILQVSRQTIGSLENGRYNPSIIL
                     AFKIARYFDMSIEEIFIYEEE"
     gene            complement(30965..31831)
                     /locus_tag="PC414_000034"
     CDS             complement(30965..31831)
                     /locus_tag="PC414_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2462455.1"
                     /translation="MEWINKLNQAITYIEENLESGVDYAEAAKIACCSTFHFQRMFSY
                     IAEVPLSEYIRRRRMTKAAFELQNSNIKILELSEKYGYDSPTSFNRAFQNIHNISPSA
                     ARAKGVVLKAYPKMTLSIYVKGNIEMQYRIVEKKGFRIVGIKESMNMIVEECFEKVPK
                     LWAKCIKNGTIDKLSELINNEPCGLLGVSVCTNSKGLDYYIAAPTDKPVLEDTHEYLI
                     PSGTWAVFECVSPMPNALAIQELQKRIITEWLPSSGYVYANLPDIELYPNGDINAPDY
                     TTEVWIPIQKPS"
     gene            complement(32057..32926)
                     /locus_tag="PC414_000035"
     CDS             complement(32057..32926)
                     /locus_tag="PC414_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2462456.1"
                     /translation="MDYKTIILNRKSVREYKNTDIKEEYLSEIKKYSESCKKLVPEID
                     VDVRIMNRSEVFNNLDKVAGYKGNMIDSPSYIIILSDIKDNYIENSGYIGESIIFKVT
                     ELGIGSCWVTFEDGKKVKEKLGINSDKEVTGIIAIGYGENVSNAKVLNATKTGQNYSK
                     SDMQVVTDKGSTRLGVEDVVYMNEWGNVASIEILEERALLDAFHYARLAPSTLNRQPW
                     RFIVDGGTVVLAVRKDGHTNLYEEKIDIGIVMLYFATIISATMFDLKWNLGTPDKDYK
                     VPEDYKIVGYCNI"
     gene            complement(33432..33653)
                     /locus_tag="PC414_000036"
     CDS             complement(33432..33653)
                     /locus_tag="PC414_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2462457.1"
                     /translation="MNVNNHKCPECGEHSKKFEIVEENEDCVIFECYTCGTISILKYC
                     ENCKEFVFVNIPNNVETYEYCCGKCNNKI"
     gene            complement(34154..35134)
                     /locus_tag="PC414_000037"
     CDS             complement(34154..35134)
                     /locus_tag="PC414_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2462458.1"
                     /translation="MGDTSRFYESVKNKYPKLKDGTRVHLWPKENIIIGPIYDEEDGY
                     KKSIGNVDSLSWKQIVILAECDGKNTTDDIINILSNRYRNIHNMKEKVMEFFMFYENV
                     YLTFEDDIQTLSSVFEITGNKSYITPLYFTIEIDGDNNTNDYFSEITSLLNCMYEKGC
                     RFIEVIGEDILKNKSMREIFQYMLDHFDLIVVTKDSFTIDRSLIKELDNYRHKVIWKV
                     YSKNDDTRIKLKEDIKITSLIKRGHTVVRGDREKAIKIEDMRFKDTKERFSKYGAEWS
                     HLYISSDGSVKSYSFQKDKRIYLGNIFNNSVEEIFLEMQNKIDKLEIVYQ"
     gene            complement(35298..36170)
                     /locus_tag="PC414_000038"
     CDS             complement(35298..36170)
                     /locus_tag="PC414_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF2462459.1"
                     /translation="MIIKVSMTVLVLILLYILYKYETQHIETTEYTIFNKKIPKEFDN
                     FKIVQVSDLHNKVFDKNNKVLIDKIESLNPDVIFITGDLVDGENKNFQVALDLIDNLV
                     EKYEVYHIIGNHEQKSLIKKYKHLYKDYFKELYSKKIINMENESVRIYRGNSHLNLYG
                     LIIPLECYPYLFANSKKLELSDNFVEDNLGKVNENEYNILLAHTPFFFEKYAKWGADL
                     VLAGHVHGGIIRIPFVGGVLSPNREFFPKYDWGKYEKDNSTMILNKGLGGSKVLIRLN
                     CKPEIVKITLKYEK"
     gene            complement(36334..36885)
                     /locus_tag="PC414_000039"
     CDS             complement(36334..36885)
                     /locus_tag="PC414_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2462460.1"
                     /translation="MDNKFPNVGENLRLLRQEMGISLDKASKMTGVSKAMLGQIERGE
                     SSPTVSTLWKISSGFKINFTTLLNENTNTYEVIKKEEVEPIVEQKGNMKLYPIYPFSP
                     QRRFELFIIELEENCTHVSSTHSHVLEEYVLVIEGKLDLNVDGKTYILESGHSIRFDG
                     TLEHIYKNLNKGKTIFHNIEVYR"
     gene            complement(36905..37807)
                     /locus_tag="PC414_000040"
     CDS             complement(36905..37807)
                     /locus_tag="PC414_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HDF2462461.1"
                     /translation="MSKKGRVVKVQHFSVNDGDGIRTTIFLEGCKLKCKWCSNPDSWS
                     NIVKLGVMKDKCVSCNRCIDVCPQNISSLFDRAQINNKCDLCGECIKVCLKDAICIMT
                     EEMSVEEIVEEVEKDFIFFFESNGGITFSGGEPTLQIDFLRELVDIFYDKGINIAIET
                     CGYFDWNKVNDVFEKIDHIFVDIKSMDDNIHKEYTGVSNKIILDNICRLSKLNKSMVI
                     RVPIIYGVNDSEENIRNTALFVKQNVPGGKMELLPYHKFGIDKYKALGLEDYIYEFDE
                     ICNNHMLKLKEIVELTGVKIIEYK"
     gene            complement(37822..40194)
                     /locus_tag="PC414_000041"
     CDS             complement(37822..40194)
                     /locus_tag="PC414_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase/glycerol dehydratase
                     family glycyl radical enzyme"
                     /protein_id="HDF2462462.1"
                     /translation="MSQTNRIEAFRQEYINSKPMICCERARIFTESHKKTEGEAICIR
                     RAKAFLETCKELPIKIFENELIVGTAGKFRRTGILTPEFSWQWVDKEMDTFDKRTQDP
                     YVISKEQIEFIRKEIFPYWKGKSLEEVFLARIPEDTAKILVDTGIIDNDSKWRQAVGE
                     VTPDYQDILFVKGYKGIKEDADKKIKELDISVSENIEKIDFYKSVSIVAQGIMTLAQR
                     YSNLAKEMSKQETDEKRKLELIKISEICMNVPANPPTNFYEAIQFVWFVQLGGILSEN
                     PLALNLGRFDQYMYPYYENDAREGKITESEAQELIEALWIKLSEWVWTISANTANYFA
                     GYNQFQNLTVGGKKRNGTDGTNDISYMCLKATESVKTHQPGLSVRVSQGAPDNFVMAV
                     AKLVKQGTGFPAIHSDSAGAQMLLQDGYDAEDARDWSNCGCVVPHFRKTGQWTSAVNI
                     NFAAALEYAMNEGKSRLTGEKMGLDTKNITEFTSFEELKDEFLKQLAYLVKSSVIGTT
                     VAQQIHKEMVPRPFLSTCVDGCLDKGVDLSKGGAKYNIGPVLTGIGLGVVSNSLAAIK
                     KLVFEDKVTTLEELTKALNNDWEGYEELRKLALDVPKYGNDNDYVDSLAIEVSDFYYT
                     ETRKYKDIFGSKFNSAFMGISNYVPTGKIVGATPCGRKATKPLTEGVSPFVGTDTTSP
                     LAAMKSASKINHDVHTGGTLLNLRLNQDLVETERGLRNLTSMIKSYFALGGFHVQFNT
                     ISNDTLLKAQENPEEYKDLLVRVAGYSTQFVNLSREMQDAIIARNSHSNF"
     gene            complement(40415..41104)
                     /locus_tag="PC414_000042"
     CDS             complement(40415..41104)
                     /locus_tag="PC414_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDF2462463.1"
                     /translation="MKVKRNINRVAIIVALNLSLALMVGCMSPVSDKNLSSSEQISKD
                     SLKEQVEDKKDESIELFNNYSQKINDAKGREEVKKDLSQRFSKAIIDSGYSITNSKSD
                     GSFVVSINGAEKPPEKNIRSLFYSMSEDDKEGKVTIDILCAKEYPEDDKLSESDKYVK
                     FIYNLFKSLTDTNLTEKEIFSMVEEDFNKGVGVVELPYMKDIHVEVNKVKQSTKVLKL
                     SLVYKFDISNH"
     gene            complement(41345..42724)
                     /locus_tag="PC414_000043"
     CDS             complement(41345..42724)
                     /locus_tag="PC414_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2462464.1"
                     /translation="MLNFMTELLVVLSIFIQFSIMNGILIYKKDSKKNEKIVFYIVLF
                     LIYIIIQISRLFNGGNYFSNINYLFYILAFIAWGGYYNYFYKMGYIKYSIFFYLFSVY
                     YKCLDAVIYRILFFAITGNVISSKESLLVPYLESIKIQIYSNLIIILIYLIFSFFRKS
                     IKLISQDKRNYVYLLFALLVNTMNMLVNYVLKKLERFGSLHSEGYYFDNFVNPKLVGA
                     SSIFLILLFKEIIKENRLKSQAELIKNKLDMQYAHYLSIQESHMRVKKLYHDINNHIY
                     CIDNLRNNSKEINEYVNNLKDEIKTFKYIYNTGNMILDIIINEKSEVCLKKGIKFTCS
                     INFSKVNFVKPIDVSSIFSNILDNAIEACDKIVDENINKYIRIKGTITRSFFVLKCEN
                     SKLNQLTFNKNILLTNKMDKFVHGIGIQSIKSSLQKYNGELLFENSIDKFILNIYIPL
                     DQDTDSWGD"
     gene            complement(42718..43431)
                     /gene="rgbR"
                     /locus_tag="PC414_000044"
     CDS             complement(42718..43431)
                     /gene="rgbR"
                     /locus_tag="PC414_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgbR"
                     /protein_id="HDF2462465.1"
                     /translation="MIKIAVCEDEKETQLLIEDYLENILKDISIEYEIQKYISGEELL
                     ESNLKDIDILLLDIKMEKLNGMDTARKIREVDNEMEIIFVTSLIDYVQEGYEVRAYRY
                     LLKPIELEELKKHVLTCIKDIEINKESHITIKNKSNTYKIYLNEIKYIEVQKKDMLIH
                     TINKNFDIKYSLGKIEKELNPYKFIRCHKSFIVNLRYVENIKPNTAILESGEEVPISR
                     YRYKEVKEKFLKFLGDTIC"
     gene            complement(43681..44241)
                     /locus_tag="PC414_000045"
     CDS             complement(43681..44241)
                     /locus_tag="PC414_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HDF2462466.1"
                     /translation="MSAMEKKIKFKFDWYSSPDKIEKWLEDIEAKGYNLYKINKFGGI
                     FYFHKGSPRKIKYTVDYYYDVENNYFDEHKSNGWNLVFRSIGSFMGTSAWYIWSKEYK
                     DIPPKLYSDSDKTNKVKSILFMNMFIFFMLVVIFIYLLNIEIRNLYSGYGASVTAIVL
                     QIILLILYAVQLYRRLMYYLRLKKFK"
     gene            complement(44336..45139)
                     /gene="zupT"
                     /locus_tag="PC414_000046"
     CDS             complement(44336..45139)
                     /gene="zupT"
                     /locus_tag="PC414_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HDF2462467.1"
                     /translation="MNNVIFAFLITLLAGLSTGIGSCIAFFAKKTNTKFLSISLGFSA
                     GVMIYVSMIEIFPKAQDALTKSMGEKLGSWSTVIAFFIGMAIIAVIDKLIPQEENPHE
                     IKKMENIEEKNIKKNKSLLRTGIFTAMAIAIHNFPEGLATFISALDDVTIAIPIAIAI
                     AIHNIPEGISVSVPVYYATGDKKKAFYYSFLSGMSEPLGAIIGYVLLRNFLNDITLGI
                     VFAIVGGIMVFISLDELLPSAREYGEHHLSIYGLIAGMGVMAISLLLFK"
     gene            complement(45290..46453)
                     /locus_tag="PC414_000047"
     CDS             complement(45290..46453)
                     /locus_tag="PC414_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2462468.1"
                     /translation="MDIKEITKSYKDYVIKLRREFHENPEKSMEEVRTSKRVKEELDK
                     IGIPYVSAGGTGVIATIKGDNPGKTVALRGDMDALQVVECTDVEYKSKNEGLMHACGH
                     DGHTSMLLGAAKVLNDIKDSINGTVKLFFQPGEEVGKGARAMIQDGAMEGVDSVFGIH
                     LWTDVESGTISVEEGPRMASADFFKITVKGRGGHGSLPHQGVDAVLASSAIVMNLQSM
                     VSREVSPLEPLVVSVGVLNSGTRFNVIASEAILEGTIRLFNPELRKQIPGILERIAKS
                     TAEAYRADAELEYGYLTPAVINDKECSKIATDAAIKLFGEDCITLFEKVTGAEDLAEF
                     MNIAPGALAFVGARNESKGACYPHHHGCFNIDEDALEIGTALYVQYAVDFLNK"
     gene            complement(46513..47700)
                     /locus_tag="PC414_000048"
     CDS             complement(46513..47700)
                     /locus_tag="PC414_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2462469.1"
                     /translation="MEAITAFCLVLVSLTIGDIVSAKTKAFVPSVFVSAIIFIVGFWT
                     FFPENIVDLAALGTPLAQLGMLLLITHMGTMMSIKELAGQWKTIVIALAGIVGICVGA
                     LALGTVVFGWDTAVIATPPLTGGLVASIMMADAATAKGLASLSVLAILMYVAQGFAGY
                     PITALMLKKEGKRLLSDFRSGKVTVNTDEEKVKDLPEQKSRFKIIPDLPEKYDTTYMI
                     ILRLGFVAWLAVQFTNLTNEVVSRYVICLIFGVIASELGIVDRKPLNKSGSFGWLMTS
                     LMAFIFAGLAKATPAMLVEMAVPLVGIIIFGVIGMGIFSIIVGKLLGESKEMAFSVSL
                     TSLYGFPPNYILTEEAVKALAETPEEKEFLMDKMLPKMLVGGFTTVTIASVVIAGIFV
                     NLI"
     gene            complement(48063..49763)
                     /locus_tag="PC414_000049"
     CDS             complement(48063..49763)
                     /locus_tag="PC414_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2462470.1"
                     /translation="MIKQFVKYYKPYKKIFTLDLIAAFLFSLCDLVYPMITRNIMDDV
                     VPNKNLRMLVVFAVALILIFIAKAGLNYFMQYWGHVIGVDMQADMRNEVFTHLQRLPN
                     TYFDNNKSGVTMSRIVNDLMDITELAHHGPEDLFISIVMLVGSFFILIDINIPLTLII
                     FAILPFIIWFAIAKKDKMNIAFMKSRVTIGDVNATLENSIAGMKVTKSFCTEKEELNK
                     FVRSNKLFRRARQDSYKVMAEYYSGMNLYMDILEWVVVIAGGYFTYIGKITLGDFAAY
                     ILYVKMFIQPMKKLINFTEQYQNGMTGFKRFIEIMEQDHQKEAKNPIELENVKGDIEI
                     ENISFTYEDKTQVLDNLSLSIKAGKTIALVGPSGGGKTTLCNLLPRFYEFDKGDIKID
                     GKSIKDVSLKSLRKNIGIVQQDVFLFTGTIRDNILCGNPNATDEEMIAAAKKARIHDF
                     VETLPDGYDTYIGERGVKLSGGQKQRISISRIFLKNPPIIILDEATSALDNVTEREIQ
                     ESLEELSKDRTNLVVAHRLTTIKNADEIIVLTDKGIEERGTHEELVNKNGVYSRLHNN
                     "
     gene            complement(50006..50281)
                     /locus_tag="PC414_000050"
     CDS             complement(50006..50281)
                     /locus_tag="PC414_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2462471.1"
                     /translation="MKKILVACGAGIATSTIVCDRVERLVKENNIQAEVEQCKIAECS
                     TKQEGADLIVSTTILPTTYDIPTIKATGYITGINTTALDKKILNALK"
     gene            complement(50400..50972)
                     /gene="mobB"
                     /locus_tag="PC414_000051"
     CDS             complement(50400..50972)
                     /gene="mobB"
                     /locus_tag="PC414_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HDF2462472.1"
                     /translation="MLLLDSFMKGHNFMYNILSVVSYSGVGKTTLIEGIIKELNKKGY
                     RVGVIKHTCHDFDMDEEGKDTYKHRKSGARKVCIISDKRVAYIEELKEKNPLYKMIQL
                     YEDMDLIIIEGYKNYRFKRLEVTRKGKYEDILSNKSDLIGIVSDIEYKLNIEQFNLND
                     YKNISKYIESCIKNEILSISNVEIKNILKE"
     gene            complement(50956..52137)
                     /locus_tag="PC414_000052"
     CDS             complement(50956..52137)
                     /locus_tag="PC414_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HDF2462473.1"
                     /translation="MIELEDAIKIIEKNVLPINRVKIVNLIDAVNKVVAEDIYSTIDS
                     PPFDRSPYDGYAYNANSENRNLKVIGTLYAGDVFNRVLNKNEAVKIMTGGKIPQGANC
                     VIKKEDVTVISDMITLNIKLKEYDNYIFKGEDIKKGQLIINKNQNIGYDGIGVLASLG
                     ISKVTVYDDLKVGILNTGTELQDINETLENGKIYDSNRYTLYSRLLKLGIQADTICNC
                     SDDILELEKKVKNMLQSVDFLITTGGVSVGDKDLIPDVFKKIGATELFWKINIKPGGA
                     CYVGTLGEKLLFGLSGNPHAAIVVFDNVIVPVIEYLTYKKGTHHIKAIFKGEFNKLSN
                     KDRFVSGKLYTSEGKLYVELNDKKDAKARLSATLRSNCMIKIKKNETIKENQLVDVVI
                     R"
     gene            complement(52348..53082)
                     /locus_tag="PC414_000053"
     CDS             complement(52348..53082)
                     /locus_tag="PC414_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2462474.1"
                     /translation="MKLKRSLVCVTILGIILVGCHKENTKEKNQVASKATQQKTMTKV
                     QNDVNEIMNKDYKYIIKNMGIPYNTFYYIKPKVLKESNTMQDINTSSYMTLVYPKYTG
                     NDELDGSALYVDINENKVVNVETNSFSSQGISVIDAESSIVIEKSDHEKSAVSLENFR
                     HIDLGEYVGVEDSRINEIVGDANYDLTAYNHEGSKVVKSYRLKEDNKILKKEVLTISI
                     VDNKIKSIKTIESDKIVKIIKGTLLE"
     gene            53444..54349
                     /locus_tag="PC414_000054"
     CDS             53444..54349
                     /locus_tag="PC414_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2462475.1"
                     /translation="MDFKQLEVFVAVAKHQSFSKAARELFLTQPTVSAHIQNLERELE
                     TVLINRSNKVITLTKSGEILYEHAIYILNNCKRAIYDIKEYSGKIEGIIDIACSSIPE
                     TYILPDFMKSFSMSYPDVKFSISHYDSQYAISEILNERISFGLVGSKINNPQIEYLDL
                     LDDELVLITPSDFKIDNKNNCIDIGELAYLNFIMRKEGSGTRNLILNTLSKNNFPVSK
                     LNVIAHVESNEAIKEMVRLGLGVSFISYISAIDYLNAGKIKCYKIKDVDFTRKFFFIY
                     SKKKTFSPLEDKFLNRLCEYFEIII"
     gene            complement(54509..55351)
                     /locus_tag="PC414_000055"
     CDS             complement(54509..55351)
                     /locus_tag="PC414_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDF2462476.1"
                     /translation="MTTSSKKLETISPDSKITRKDFWKCFRRSLTLDSSWNYERMQNI
                     AYAYMMAPIIRRLYKDDKEKKSKALKRHLEFMSVTPHISTLLVGISGAMEEENAKNKE
                     FDANSINAVKSSLMGPVSGIGDSFFWGTLKLIAAGVGIALASQGNIMGPILFLLIINV
                     PHFIIRYICLDKGFKYGTQFFKDVSGSSIVSKVMEAASMLGLMVIGGMTASNVMLKLS
                     VNVGSGEWAEPIQTYLDQIMPCMLPAMIFGIMYWLLGKKVKTTTILISVMIICIVLAA
                     IGVV"
     gene            complement(55335..56093)
                     /locus_tag="PC414_000056"
     CDS             complement(55335..56093)
                     /locus_tag="PC414_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2462477.1"
                     /translation="MEFTQVILITLIAFFAYMHSFVGSTMHNRPIVVAPLVGLALGNL
                     HTGIVIGSTLELVFMGAFPVGASNPPDFVSGSIIATAFVILTGQDVSAAVVLAVPIAT
                     LVLLIDNFLMTVVLTWGAHIADRYAEEGNIEGVERVQLLFGIGNKLILAIIVGIGFSL
                     GVPVIEKILSFIPSYVTHGMDVAAGVIPAIGFAMLARMMLNKKTVAFLLLGFILVAYL
                     NITVTGVALFGLAIALIYVNFVGEKEVIVDDNEF"
     gene            complement(56110..56580)
                     /locus_tag="PC414_000057"
     CDS             complement(56110..56580)
                     /locus_tag="PC414_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2462478.1"
                     /translation="MIKLVRVDHRLIHGQVAFTWTKFLSTDCILIASDDLLKDELRMA
                     GLKMAKPSNVKLVMKSIADSIKALNSGVTDKYNLLILCESVEDVYRLAKEVKAIKSIN
                     LGGTKSDDNRENISKAVHVSKDDIKMIKELDSEGVNVFVQLVPDDDATNVMKLI"
     gene            complement(56580..57323)
                     /locus_tag="PC414_000058"
     CDS             complement(56580..57323)
                     /locus_tag="PC414_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane biogenesis protein"
                     /protein_id="HDF2462479.1"
                     /translation="MKKKGILSVFKSKKPIIAMIHLKGDTPEDIFERAKKEITIFEEN
                     GVDGIMLENYYGNYYDLERILEYVSKANLSIPYGVNCLNVDTMGFELATKYNASYIQV
                     DSVVGHVKPRDEATLEEFFKLQRSKCPAYLIGGVRFKYQPVLSENDVEEDLKIGMTRC
                     DAIAVTENATGQETSMEKIELFRKNLGDFPLVIAAGVTLENAKKQLKLGDMAIIGSYF
                     KDNYKDFGDVSVEHVKTFMDEIKKIREEL"
     gene            complement(57327..57740)
                     /locus_tag="PC414_000059"
     CDS             complement(57327..57740)
                     /locus_tag="PC414_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2462480.1"
                     /translation="MKKIILASHGDFSKGLLNAVKMIVGDLADCVSSYGLYPGQSASD
                     FALELEKTISEDKECEYIILSDLYGASVCTAMLRLTNLHNVRLFSGMNLNMVLELLTR
                     FSDELTVEDIDQLVEESRRGIQSVTLQIKEEEEVF"
     gene            complement(57766..58518)
                     /locus_tag="PC414_000060"
     CDS             complement(57766..58518)
                     /locus_tag="PC414_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2462481.1"
                     /translation="MDEKRQKKEMDLEVNSFVPLYQQLYDNIKKQIASGIYKPGDKLP
                     SEGDLCKEFNISRITVRNALNELVKEDILCKKRGKGTYVTIPERIEATCAGNSFTNSC
                     HRINAKPSTKIISVLIKKADKQVAEALSIELEEKVICIKRLRLIDEVPVIFEVDYFRI
                     DYMFLLKEELEDKSLMEVISNNISTLPKRVENIFEVKHSNKEYSDHLKCASNMPLLKV
                     RQSVYTENNDVLYYNEQFIRSDKYKYAVSAEI"
     gene            complement(58758..60152)
                     /locus_tag="PC414_000061"
     CDS             complement(58758..60152)
                     /locus_tag="PC414_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDF2462482.1"
                     /translation="MNLKYEFKNAWDFERKENTIEQIMQYSSNYMEFLSKSKTERLSV
                     KEIIKLAKENNYISIDEAMEKGSISCGDKIYVINKEKAVALFVIGKNYIEKGMKIIGS
                     HIDSPRLDLKPNPLYQESNLGFFKTHYYGGIKKYQWTAIPLALHGIVILNDGTKVDIS
                     IGEEDSDPVFCVTDLLIHLAGDQMQKKLSEGISGEALNILIGNMPLEDEEKEPITANI
                     LKILNEKYNIVEEDLLSAEIEVVPAGKARDLGLDRSMVLGYGHDDRVCSYAAVKAILE
                     TEQPEFTSVALCVDKEEIGSKGNTGMHSKFFENTVAELIALEGDYCDIKVRRALANSK
                     VLSADVSAGYDPNFGEAYEKRNSAYMGNGVVLTKYTGSRGKSGCNDANAEFMSEVKRI
                     FNKGNVIWQTAELGKVDQGGGGTIAHILANQGAEVIDCGVGVLNMHAPHEIVSKVDIY
                     EMYKGYKAFFNINL"
     gene            complement(60172..60381)
                     /locus_tag="PC414_000062"
     CDS             complement(60172..60381)
                     /locus_tag="PC414_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HDF2462483.1"
                     /translation="MFDKKKLDRINELAKKNKEGILSADEIKEREILRKEYLENFRAH
                     FRSRLDSVKVVSPEEYEQYMKNNKN"
     gene            complement(60410..61855)
                     /locus_tag="PC414_000063"
     CDS             complement(60410..61855)
                     /locus_tag="PC414_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF2462484.1"
                     /translation="MEKYRIDLNEKKSTKYIQIFNHIKELIINKELVEHEKLPPIRKL
                     SNFLQVNNTTIVKVYELLENEGYVYKIIGSGTFVSSLNSYKSGIDNRKNKIIVQREDL
                     IHFDNGNPSNDMFPIDSFKNAVNMALSKDGSSIFEYDEGLGSEELREKMVEYLSDGNI
                     KTTKENIQIISGAQQGIDIVCKGLISYSDVVFMEEPSYNGAIEVFKSRGAKIISIPML
                     DDGIDIGILKLKLEKIKPRLIYIMPNFQNPTGISYSTYKKKKLIELAEEHDFYIIEDD
                     FISDFVFESKDNRTIRSYDDKNRVVYIKSFSKILMPGLRIGIVEMPNELLKKVLWAKY
                     SSDISTPGLIQKSMYYYMNNFDWKNYLSHIEKIYTDKYKLAKNLINDKLSGKLKVRKS
                     CGGINFFLELPRGYTSQAFTNFMLNKGVSMLPGTYFFDNLVDDRFFRINIARPSMEEL
                     EKGISIISDNIDEFFCEYKNKLDIKSNKLFY"
     gene            61965..62759
                     /locus_tag="PC414_000064"
     CDS             61965..62759
                     /locus_tag="PC414_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HDF2462485.1"
                     /translation="MFYDYHMHSSFSTDGKSTMEEMVKKSIELGLEEICFTDHVDYDV
                     YADDSFSIVYEDYFKSLETLQSKYKDKISIKKGIEFGVQTQLMDTYKKEAHQYPLDFI
                     ICSIHAIDTMDLYLGNYFKDKTQHEVYENYYLYLYNIVKNYKDYSVLGHLDLIKRYAP
                     YDTILDDRLFSDIIEETLKQAIYDGKGIEINTSCYRYNLPDLTPSKYILQMYKDLGGE
                     IITTGSDSHHISQVACEFDYIYSLLKNMGFKYVSKFNKLKPEFIKL"
     gene            62810..64132
                     /locus_tag="PC414_000065"
     CDS             62810..64132
                     /locus_tag="PC414_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDF2462486.1"
                     /translation="MKVPYLVLSTVILFISNFIVRIFGFLYKIFLSRALGETGLGIYH
                     MIFNFLMICLAVTTTGIPTALSCLVAKRKALNDRHNTNALFISTLYISFFVALIISIV
                     ASFNSSFLSLKFLKDAKLNLFILAVCPAIVIITLSNVLRGYYYGIKNVKIPAIGQIIE
                     QIGKILFVFLLVMYINNKSMNCYIALLGISIGELSNIIFMLICLWRDSSFDNRYIISI
                     KDFYNSSMETLKMSIPITCNRMSNILLQSISSMMIPSRLALSGMTYQQSLSMYGIVSG
                     MVMPFIFLPFTVGSALIVNLIPTISQEMALRKRKSVIKKIKYSILLTLFVGILSSIFF
                     YFFGKDLCILVFKNTLAGEYLKAMFLVPLFMSLNQTLSGILHSIRKELASSINTITGM
                     LIQLIALYVFLPIPGLNIYAYIYTMTIVSIFTCLLHTIVLFKSLKSIR"
     gene            complement(64233..65450)
                     /gene="cdeC"
                     /locus_tag="PC414_000066"
     CDS             complement(64233..65450)
                     /gene="cdeC"
                     /locus_tag="PC414_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeC"
                     /protein_id="HDF2462487.1"
                     /translation="MQDYKKNKRRMMNQPMSTMNEEEVYTDEINSEDMRGFKKSHHHN
                     GCNTDNKCECHDDCNPCNPCNPCKPNPCNPCKPNPCDDNCGCHDNCKCDCEPCEMDSD
                     ECFENKCGPECCNPISPRNFSVSNAVPFAIEANRIFDTMQFQTFTDATGPNGEPLTFE
                     TEVVEVFGSVPSAGQASVTIEKICLSNDGIVIDTGMTTLEDFDLDPLGDIVGRNCETT
                     FEFAVCGERNAECCRQGKGKSVAYKQRGLTVAVRNLVLELRGRCGCTEFVALAFPAVR
                     AGGGCKRRVDYVEFTFNTLSAPICLPADGRAVTLRQEYQTNLTVDCIGKSILKLECNE
                     CCEPFYELIIPNDIDLVLCLQETVSTLISEQIVVLASPNPIQPRLVDTFSKVCDFSQC
                     GPNHGSGKPSCHR"
     gene            complement(65633..65813)
                     /gene="ssrS"
                     /locus_tag="PC414_000067"
     ncRNA           complement(65633..65813)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PC414_000067"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(65928..66839)
                     /locus_tag="PC414_000068"
     CDS             complement(65928..66839)
                     /locus_tag="PC414_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2462488.1"
                     /translation="MNNLLFLIGVIALSNGNISIFDDKNKSKGNNRVRKNKKNANDKL
                     SQSTHGNLRFDANDIKRGLRLMDLSEEDLEMGMEIITRTKKYMSRDERKILIKIESVL
                     DLVRGIKKLNNIDMVDTEDETDFFRKMDSDDKKNMMIKEIIDVFPEKRKNSVEKAIGM
                     KKKIDLFAELFLPDDFGEGGFSLSSLANINNLGSMNNLKLLGSLLRGDDSNNDDEDNN
                     DELNYEDEELDCEDKELDDDYDELTDEGEMEDNEDELVEDKNTGYENIENDEDIVYDE
                     ELYNINEQESEYNYNEKNTKTRAKKKR"
     gene            67051..67449
                     /locus_tag="PC414_000069"
     CDS             67051..67449
                     /locus_tag="PC414_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2462489.1"
                     /translation="MKKLDTESLEKMAKQKNIDKDKIEKMADSYKGKSENELMEELIK
                     IGKNLDGRDEVVSKFKAFLDENQRKKLDNIMEKISDAENQRDTKPAKTKKAKPTKGKS
                     NSPAPKNTPGSQQKKSKSLFKKTKKSNPHE"
     gene            complement(67572..68588)
                     /gene="ccpA"
                     /locus_tag="PC414_000070"
     CDS             complement(67572..68588)
                     /gene="ccpA"
                     /locus_tag="PC414_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator CcpA"
                     /protein_id="HDF2462490.1"
                     /translation="MKGNITIKDVAKQAGVSISTVSRVINDSKPVTDEVKQKVLEVIK
                     ETGYIPNPLARSLVTKKSQLIGVIVPEVSDSFVNEVLNGIEEVAKMYDYDILLANTYS
                     DKEQELKSINLLRAKQVEGIVMISWIVEQEHINYIQNCGIPATYISKTARNYDIYTVS
                     TSNEEATFDMTEHLIKKGHEKIAFIMTSKDDTVLEMERLAGYEKALSNNNIELDKSLI
                     KYGGTDYESGYNSMKELLDDGIIPHAAFVTGDEAAIGAINAICDAGYKVPEDISVAGF
                     NDVKIARMYRPKLTTVYQPLYDMGAVAIRMVIKLINKELIENKKIELPYRIVDRESVT
                     ERKK"
     gene            complement(68841..69041)
                     /locus_tag="PC414_000071"
     CDS             complement(68841..69041)
                     /locus_tag="PC414_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2462491.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFEDCWRDIDIMSWIPF
                     LILLLITCSCGGFFDGEVGCGC"
     gene            complement(69075..69254)
                     /locus_tag="PC414_000072"
     CDS             complement(69075..69254)
                     /locus_tag="PC414_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2462492.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFGDSWAEFDIMAWIPF
                     LIVLLILCCCGGCLE"
     gene            complement(69436..69642)
                     /locus_tag="PC414_000073"
     CDS             complement(69436..69642)
                     /locus_tag="PC414_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2462493.1"
                     /translation="MTKLEDNMERFFGGCEGFFGNKMLIIIVIVFLLLCTDILEDLLC
                     DDNIWIWVILIVLLLFNFDDGCCC"
     gene            complement(69690..70115)
                     /locus_tag="PC414_000074"
     CDS             complement(69690..70115)
                     /locus_tag="PC414_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2462494.1"
                     /translation="MKYVGLLLTSVGMFLLIAVNFYYNSITLDMQRIEDYVMETNLIL
                     EDVAEKESYVSNEKEDYISRLMHVKKGIENSKTSFLIERYKEYKIKSIESLIYTISEE
                     KKDYLDEVDRYNKLGEKEINKLINKNFLEVTYLSITTYI"
     gene            70287..70514
                     /gene="acpP"
                     /locus_tag="PC414_000075"
     CDS             70287..70514
                     /gene="acpP"
                     /locus_tag="PC414_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDF2462495.1"
                     /translation="MFEKIKEIIAEQLGVDNLDEITMEASLMDDLEADSLDAVEIIMA
                     LEDEFGIEIPDEEAENFKCIGDICKYIEENK"
     gene            complement(70658..71662)
                     /locus_tag="PC414_000076"
     CDS             complement(70658..71662)
                     /locus_tag="PC414_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HDF2462496.1"
                     /translation="MIIHKFIIHVLDKNSDVPILNDFEGKVNQEVDGFFQKAIKRIAK
                     DEDLRKGVFKDYNDNLIKNCCEQIIYDESTFLKNSKEIASYLFDVMKINAALESCDLA
                     ICLYTIKDEKSVAILKLDYKKLYTHSIEFVDDKFNIQMVSNEIGIPETLRQKQGALIS
                     LSGINDEFHLRLLDKDAEKEGSESKFVTEFLNAKKIDDDKYKTKVFKNTAENWITNAL
                     SNDIKQAEDVRSILNYTLKEKHEVDINDFVDNSIKDDELKDSFKEHMEEKGLDESFSI
                     DKKWVEKKLKKRSIKTDNGFDIKGNLTDFEDPMKYTVKQNQDGTIDIIIKNVTFYEEK
                     "
     gene            complement(71676..72578)
                     /locus_tag="PC414_000077"
     CDS             complement(71676..72578)
                     /locus_tag="PC414_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDF2462497.1"
                     /translation="MFKKENQRYNLISYTNEEEMTLKEVLLDKLNFSVRSLSKMKREK
                     SVLVNGVYKKPSLKVYSGDLIEVKIDEEKANFEPQDLNLQIIYDDFDIIMVNKPPFMV
                     VHPTKSHYDKTIANGISYYIDNQKENVKIRFVNRLDMNTSGLVIVAKNAYAHHTLSTA
                     MSENKVEKKYITVVDGIIKENEGTIDEPIYRPTEDSIKRIIDERGQSSVTHYKVIERL
                     ENATVLEVSLETGRTHQIRVHMAHIGHGIIGDELYGYVDEELINRQALHAYKLEFEQP
                     RTKEKLKFKADIPEDMKELISKLR"
     gene            complement(72773..73948)
                     /locus_tag="PC414_000078"
     CDS             complement(72773..73948)
                     /locus_tag="PC414_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HDF2462498.1"
                     /translation="MREVVIASAARTAVGSFGGAFKSVSAVELGVTAAKEAIKRANIT
                     PDMIDESLLGGVLTAGLGQNIARQIALGAGIPVEKPAMTINIVCGSGLRSVSMASQLI
                     ALGDADIMLVGGAENMSMSPYLVPSARYGARMGDAAFVDSMIKDGLSDIFNNYHMGIT
                     AENIAEQWNITREEQDELALASQNKAEKAQAEGKFDEEIVPVVIKGRKGDTVVDKDEY
                     IKPGTTMEKLAKLRPAFKKDGTVTAGNASGINDGAAMLVVMAKEKAEELGIEPLATIV
                     SYGTAGVDPKIMGYGPVPATKKALEAANMTIEDIDLVEANEAFAAQSVAVIRDLNIDM
                     NKVNVNGGAIAIGHPIGCSGARILTTLLYEMKRRDAKTGLATLCIGGGMGTTLIVKR"
     gene            complement(74035..74880)
                     /locus_tag="PC414_000079"
     CDS             complement(74035..74880)
                     /locus_tag="PC414_000079"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HDF2462499.1"
                     /translation="MKLAVIGSGTMGSGIVQTFASCGHDVCLKSRTQGAIDKCLALLD
                     KNLTKLVTKGKMDEATKAEILSHVSSTTNYEDLKDMDLIIEASVEDMNIKKDVFKLLD
                     ELCKEDTILATNTSSLSITEIASSTKRPDKVIGMHFFNPVPMMKLVEVISGQLTSKVT
                     FDTVFELSKSINKVPVDVSESPGFVVNRILIPMINEAVGIYADGVASKEEIDEAMKLG
                     ANHPMGPLALGDLIGLDVVLAIMNVLYTEFGDTKYRPHPLLAKMVRANQLGRKTKIGF
                     YDYNK"
     gene            complement(74978..75775)
                     /locus_tag="PC414_000080"
     CDS             complement(74978..75775)
                     /locus_tag="PC414_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HDF2462500.1"
                     /translation="MSTSDVKVYENVAVEVDGNICTVKMNRPKALNAINSKTLEELYE
                     VFVDINNDETIDVVILTGEGKAFVAGADIAYMKDLDAVAAKDFSILGAKAFGEIENSK
                     KVVIAAVNGFALGGGCELAMACDIRIASAKAKFGQPEVTLGITPGYGGTQRLTRLVGM
                     AKAKELIFTGQVIKADEAEKIGLVNRVVEPDILIEEVEKLAKIIAKNAQLAVRYSKEA
                     IQLGAQTDINTGIDIESNLFGLCFSTKDQKEGMSAFVEKREANFIKG"
     gene            complement(75794..76804)
                     /locus_tag="PC414_000081"
     CDS             complement(75794..76804)
                     /locus_tag="PC414_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDF2462501.1"
                     /translation="MGNVLVVIEQRENVIQTVSLELLGKATEIAKDYDTKVSALLLGS
                     KVEGLIDTLAHYGADEVIVVDDEALAVYTTEPYTKAAYEAIKAADPIVVLFGATSIGR
                     DLAPRVSARIHTGLTADCTGLAVAEDTKLLLMTRPAFGGNIMATIVCKDFRPQMSTVR
                     PGVMKKNEPDETKEAVINRFKVEFNDADKLVQVVQVIKEAKKQVKIEDAKILVSAGRG
                     MGGKENLDILYELAEIIGGEVSGSRATIDAGWLDKARQVGQTGKTVRPDLYIACGISG
                     AIQHIAGMEDAEFIVAINKNPEAPIFKYADVGIVGDVHKVLPELISQLSVAKEKGEVL
                     AN"
     gene            complement(76823..77605)
                     /locus_tag="PC414_000082"
     CDS             complement(76823..77605)
                     /locus_tag="PC414_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HDF2462502.1"
                     /translation="MNIVVCIKQVPDTTEVKLDPNTGTLIRDGVPSIINPDDKAGLEE
                     AIKLKEEMGAHVTVITMGPPQADMALKEALAMGADRGILLTDRAFAGADTWATSSALA
                     GALKNIDFDIIIAGRQAIDGDTAQVGPQIAEHLNLPSITYAEEIKTEGEYVLVKRQFE
                     DCCHDLKVKMPCLITTLKDMNTPRYMKVGRIYDAFENDVVETWTVKDIEVDPSNLGLK
                     GSPTSVFKSFTKSVKPAGTIYNEDAKTSAGIIIDKLKEKYII"
     gene            complement(77664..78800)
                     /locus_tag="PC414_000083"
     CDS             complement(77664..78800)
                     /locus_tag="PC414_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HDF2462503.1"
                     /translation="MDLNSKKYQMLKELYVSFAENEVKPLATELDEEERFPYETVEKM
                     AKAGMMGIPYPKEYGGEGGDTVGYIMAVEELSRVCGTTGVILSAHTSLGSWPIYQYGN
                     EEQKQKFLRPLASGEKLGAFGLTEPNAGTDASGQQTTAVLDGDEYILNGSKIFITNAI
                     AGDIYVVMAMTDKSKGNKGISAFIVEKGTPGFSFGVKEKKMGIRGSATSELIFEDCRI
                     PKENLLGKEGQGFKIAMSTLDGGRIGIAAQALGLAQGALDETVKYVKERVQFGRPLSK
                     FQNTQFQLADMEVKVQAARHLVYQAAINKDLGKPYGVEAAMAKLFAAETAMEVTTKAV
                     QLHGGYGYTRDYPVERMMRDAKITEIYEGTSEVQRMVISGKLLK"
     gene            complement(79509..79976)
                     /locus_tag="PC414_000084"
     CDS             complement(79509..79976)
                     /locus_tag="PC414_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HDF2462504.1"
                     /translation="MSISNVREYFKQFGKEDSILEFEQSSATVELAAEAAGVIPARIA
                     KTLSFKIGDDAILIVTAGDAKIDNKKYKAEFNCKAKMLTPEEVLEFTGHAIGGVCPFG
                     LKNSIKVYLDDSMKRFDTVFPACGSSNSAIELTCEEMEKFSKCEKWVDVCKNW"
     gene            complement(80055..80597)
                     /locus_tag="PC414_000085"
     CDS             complement(80055..80597)
                     /locus_tag="PC414_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2462505.1"
                     /translation="MKKFIKVSFFILGIFTLFIAINKLVYRDMVSVKDYEINHLEEIV
                     FDFDSRRSRDVGTIVRFKISNNSKYSYKLNSAKMKFENCVENKKGMNKCSAYVNVDLF
                     NEKNSITERTLKYGIKPNNQGYVDFVIPKGLNFDYRYFNESGMSVEYKGEYIVNIPMA
                     KGLYLVVGKNNDTWNANIME"
     gene            complement(80649..81335)
                     /locus_tag="PC414_000086"
     CDS             complement(80649..81335)
                     /locus_tag="PC414_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HDF2462506.1"
                     /translation="MFNLIRKDLIIGISSDGIRNLKYILLFFVFYFFLNSISYYTVSI
                     VVSYLIFINTFECDYENDSRIFIRSMPVSIEDIVYSKYLLGVGLIISVTIIVSLLSKL
                     TSLVFFRNMVLNDVFFSVNIFLAILTILLPLIFKFGYGKMKVCGFIVSILIYFVYGSL
                     LKMISMIVYQVKHFNYSKVGGVYLSNYVTDIANTKYINLYSMTFLTIIIFIISMYFSI
                     KISKKNKFNY"
     gene            complement(81335..82195)
                     /locus_tag="PC414_000087"
     CDS             complement(81335..82195)
                     /locus_tag="PC414_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2462507.1"
                     /translation="MFSIRNITKKLGKFKLNNINLELKEGDIVGIIGPNGSGKTTLIK
                     IIMGIIDADEGEIELCNETIENSPISFKNNIGFVYDSLQFYPHLKVKEFRKIVSLFYK
                     NFDRERFDEYLNKFDIEENMHIENLSKGQSEKLMLSSALSHNAKLLILDEPTAGIDPI
                     VRTEIMQYLQDFVKNGSSSVIISTHNTDNLIKIADYLVFINRGNQIFTVKKELIEQEY
                     KIIRANKAELEAIKESIVGVKEYKYYNEALVKVGDSLKVKSLLIEIDKHKVKNPTIEE
                     LMYYYVNEVR"
     gene            82330..83130
                     /locus_tag="PC414_000088"
     CDS             82330..83130
                     /locus_tag="PC414_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HDF2462508.1"
                     /translation="MKRIITINTNQLNKSLETKELLTRKLINAGFEVYSDIYPDTELI
                     ISIGGDGSFLRTVRDFDFPEIPIMGINTGHLGFFPDILPDKIDSFIEAYTKKDYIIQE
                     MSLLNAEVYTTTSGSNMLAVNEVVIRGDKSRTIHLNLSLDNKHIQNFSGDGMIISTST
                     GSTAYNYSAGGSIVDINLELMQITPLHPINTNAYRCFTSSIICSNESVIKIAPEYRFE
                     DSVLIVVDGVEHRFRQIENIKVSISDAKIKLLRMSNYEFWHRVSEKFL"
     gene            83155..83724
                     /gene="yfcE"
                     /locus_tag="PC414_000089"
     CDS             83155..83724
                     /gene="yfcE"
                     /locus_tag="PC414_000089"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HDF2462509.1"
                     /translation="MKIGIMSDTHGSLLYFEKALNVLSDCDVLLHGGDVLYHGPRNDI
                     PEGYNPKKFIETLNKLENIVIVKGNCDADVDQMVIEHPIQSPYVMSQFGEIRIILNHG
                     YIESEEEIIDKAKKMGGDILVLGHTHVKKLYMDDNLIVINPGSTSIPKDGSHSVAIID
                     IIKTDDEDELELDINLIDINTGNIININD"
     gene            complement(84059..85111)
                     /locus_tag="PC414_000090"
     CDS             complement(84059..85111)
                     /locus_tag="PC414_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp18"
                     /protein_id="HDF2462510.1"
                     /translation="MKIKSKKIIAFALSLGIMLPSCVYADTISKNQNAQVDMNNYLQG
                     RKSPKEEKLIGEDRFDTAIKISQSGWNNGSERVFLVNSNSLPDALASTPLASKLDAPI
                     LLTNKNSIPYNVTEEIKRLNPAEVILIGSEGAISSSVKESLEDMEVLVSRIGGADREE
                     TSLLLTRQLDDTGDLGVSKVAVVNGYNGLADATSISSPAASDNTAIIYTGKDSIRSEA
                     KNFITQNSTKTYIVGGEYSISKKLEGQLVNSERLAGTDRKDTNAKVLEKFYGKSSKVN
                     NMYFAKDGSGREADLVDGLAAGVLASKTKSPVVLASGSLSSAQKSFIKKVKADKFVQV
                     GGGKNSKPYAEALALQ"
     gene            complement(85318..86544)
                     /gene="pepT"
                     /locus_tag="PC414_000091"
     CDS             complement(85318..86544)
                     /gene="pepT"
                     /locus_tag="PC414_000091"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDF2462511.1"
                     /translation="MKQKVVERFLKYVSFDTTSNSQCENCPSSEGQRVLAKYIVEELK
                     TMGVDDVSLDENSYIMATLKGNTDGVDTIGFISHLDTIEDVSGKDIKPRIIENYDGKD
                     IVLNEALNVITYVKDSPELEEFKGDDLIVTDGTTLLGSDDKAGIAEIVTAIEYLINHP
                     EIKHGDIKIGFTPDEEIGRGADLFDVEKFGAKYAYTLDGGIVGELECENFNAANATIT
                     IHGRNVHPGSAKNKMVNAIHIAAEISEMFPADERPETTEGYEGFWHLNSIGGNVENVS
                     MAYIIRDHCKEKFENRKSIMIENIEKINKKYDNRVELDLKDSYYNMKEKIEPVMFIVD
                     IAKEAMEELGIKPRLVPVRGGTDGARLSFNGLPCPNIFTGGLNFHGKNECIPVSSMEK
                     ATKLIVRIAEKYAERV"
     gene            complement(86634..87686)
                     /gene="ytvI"
                     /locus_tag="PC414_000092"
     CDS             complement(86634..87686)
                     /gene="ytvI"
                     /locus_tag="PC414_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HDF2462512.1"
                     /translation="MSILENNFLYKLKNNTIFFCIYTLLFIFIYKAFPYIAPFFLGAI
                     IALMINPISQKLENKFRINKGISTLVLSFLAVAIVSTVTTIIVINSMKELMGFLNNIS
                     ANPEDISNTIMYFLNKINDFMKSFQEIANFDVEQLVNKFSGEVMQITKNLLTSILGLA
                     TSIPYIIIFIITLFIATYFIAKDLDKIENSFYNMFTVDVRKKVKNVKKEAGLSLVGYI
                     RAYTILMAITFFAIWGSFALFGLKYGLIVGFVGALMDLIPFLGIITIYLPVIVYYFLI
                     KNYFIAISMTVIFFVLSLIREILEPKLVSVNVGLNPLATLAAIFIGIQVKGIIGVIFC
                     LGLVCMHDILKKVDIF"
     gene            complement(87835..89088)
                     /locus_tag="PC414_000093"
     CDS             complement(87835..89088)
                     /locus_tag="PC414_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDF2462513.1"
                     /translation="MLESPNNLIQIIFLIVLLIGSAFFSASETALMSLSKIRIRYMQD
                     EGVKGAKLVSSLIENPNKLLSSILVGNNVVNIAATSISTSLFIGLMGEKGVALATAVM
                     TVLVLIFGEITPKTIAANNSEKVSLLVSKPIKAIIFILRPIVWIFNIITNIIFKLFGI
                     TNKGAKSFITEEELKTMVNVSHEEGVLEMEEREIINNVFEFGDMQAKNAMVQRIDMVA
                     IDMEDSYDEIIQVFKTEKLSRMPVYEETIDDIVGILNIKDIIFLSDEEIESFDIKNYM
                     REPFFTYEFKKITQLLEEMKLEKSQMAIVVDEYGGTSGLLTIEDLVEVIVGDIEDEYD
                     EEEDEIQVIKEDEYIVDGSTKIGDVNELIGVNLESEEFDSIGGFIIGHLSRLPEENEV
                     IEVDNIRFCIESIEKNRIKKIRIYT"
     gene            complement(89219..92746)
                     /locus_tag="PC414_000094"
     CDS             complement(89219..92746)
                     /locus_tag="PC414_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2462514.1"
                     /translation="MRPIRLELTGLNSYIDKQVIDFEKLIERGLFGIFGTTGSGKSTI
                     LDAITIAMYGNISRNTKEYINSVCDKAIISYEFEIGSKNTRRRYIVDRTIARSKTGTK
                     TSNARLIEVLNDNTQNVLADKVVEVNEKVAQVVGLTANDFTRSVVLPQDKFNEFLRLS
                     RADRRDMLERIFNLEKYGRSLGEKVKKRKNIQLQNLKDLKSKLSQYDGITEEVYNNIN
                     QELIELKNLEKDKDNALDLAQKSYEESKTVYEEQLKLEKNELRKNELDLKNSEIKEKI
                     ALVENDSNAKKVDPYISSVQNLEKKIDEDSFAVSNLEKKLAILNQELEVTKNRYEKIN
                     KIKNEEVPKLSEEKIRLQQAIKLEEELVLLDRELKELKESGINLNKTKVELEKVKQVS
                     ESRKDAVTKSIKEVEGKIDKVNISAELKQKIFLAYEYEKDYNNVLEEKNQKLNKLEEI
                     LKETENINLKVRYIDKDKNDVNRNLENLSLHLDVLLKKCPGKSADLLLKSEYVTELRN
                     KANNTKENEIKKSSIQDELKIILESKFNTEREVNLLNEKLENNRKNRDDLEKELEELK
                     YLNLASELRRELKENMPCPVCGSKHHENHDITKYDENISFVKEKLEKLEKEKISIRNN
                     IEELNAKVSGYLSIEKMKTKELEDVKGKLGGIPSSQLLKKLDEEQRKLALLKSNIQEW
                     EKEKESTENKVTLAKEEKNKIEKEELKIRESLNNYKKLTRDLNIEIEGLENKCKKLKQ
                     EYLGLKTITKVSNLLSKVKEIRENEKELELLSTGHSNLLKNRDSLDIKIRENESKLHE
                     IEIELIKARELYVEKKLSRDNKYKEVISITKGDLAKNLLHNVEENICKILEQEESSKK
                     KLEEQRLEYEKNIAEKHNIDGRLKTAKEQYKDQKSTLNKLLAENKFESIYAVKRALLD
                     TDAVKKLIEEILEHEEEQKLLSFKIKSSKEKLNGRSIKKEYFEQLKDEIYNLKVEIGN
                     ISKDIGANQNQLITLKDSLDKINDFNKQLKVVEHNVDLLEELDKCVQGNKFVEYVSTN
                     QLKYIALEASKRLDGITKGRYALEIDSTLNFVMRDNFNGGERRSIDTLSGGETFLTSL
                     SLALALSSQIQLKGSAPLEFFFLDEGFGSLDSELLEIVIESLERLHSNNLSVGIISHV
                     EELKNRVPVKLLVSSSEAGIGSKVKIEYS"
     gene            complement(92733..93944)
                     /locus_tag="PC414_000095"
     CDS             complement(92733..93944)
                     /locus_tag="PC414_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HDF2462515.1"
                     /translation="MRFIHTSDWHLGKSLEGHSRIEEQAKFCEEFIKIVESNEIDMVI
                     IAGDVYDTSNPPAQAEKLFYQTVSRLANNGQRCVLIISGNHDNPERLSAITPLAHEQG
                     ILIYGYPLSATIEAKYKGFEITYATQGCTKLNINGENIVVATLPYPSEKRLNEVFSSE
                     DEFEKQKNYSEKVGDIFRSLEENFRSDTINIAVSHIFVIGGESTESERPIQLGGSFLV
                     ERKDLPEKAQYTALGHLHKQQKASERLNAYYSGSPLQYSKDERAYTKGAYIVDIKAGE
                     KPIIEDVYFNNYKPIEVFKCNGIEEALDICEQNQDREIWSYFEINTDEIISQNEIKKM
                     KELLKDIIEIKPIITSCYEQESVDIKEKSMAELFREFYSFSKGVEPKGELMDLFLDII
                     SEEGESADETN"
     gene            complement(93990..97817)
                     /gene="addA"
                     /locus_tag="PC414_000096"
     CDS             complement(93990..97817)
                     /gene="addA"
                     /locus_tag="PC414_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HDF2462516.1"
                     /translation="MSSPKWTKEQLEVIESRECNLLVAAAAGSGKTAVLVERIIQMIT
                     SRENPIDIDKLLVVTFTNAAASEMRERIGDAIGKALDENPENKHLQNQLVLLNKSSIT
                     TIHSFCLDVIKSNFHRINLDPNFRIGDQTECAILKQEAIEEVFEDLYEERDEGFLNLV
                     ESYAERGGDKEVQDIILGIYSFAMASPEPKKWLIDSAERFNIDENFDFSQSIWARAIL
                     DTVKIEINGLCLNMERALKEVESIEELETFAEKLSVEYKKIADISQACNKSWDEAYKK
                     MASMSFENYVKGVKRISKDAPSYIKESKEKAKTIRDKTKKSLESIVSATFNKDNDSIR
                     EEIKYLYNIVKPISSVVLRFEEEYSNKKREKGIIDFNDIEHFALNILTDVDEKGNIVP
                     SDIAVGCRNKFYEIFIDEYQDSNLVQEVLLKAVANTETPNRFMVGDVKQSIYRFRQAK
                     PELFLQKYNNYNDKKGSSHRKIMLYKNFRSREEVVDAVNYIFENIMNENIGEIEYTEK
                     ERLNLGANFNVDTDEKSIIGGATEIHLIQKDNKLDDDIINDKDDRINNKENEIEEEEK
                     LDNIQLEARMVGNIIKDLMKVNEDGKIQKVYDKGIDGYRPVEFRDIVILLRATSAWAP
                     VFADELMNMDIPTYADVGVGYFDTIEIKTILSLLQIIDNPMQDIPLISVLKSPIFGFT
                     PEDLIDIRVQSKDKIFYEVLKSTAEYDGFTDSQNENESEFIPSEECINKSKDFLIKLK
                     EFKEKSMYMSTDEFIWYLYTRTGYYAYVGALPGGSQRQANLKVLFERAKQFEETSLKG
                     IFNFVNFIEKLKKSSSDMGSAKTLGENANVVRIMSIHKSKGLEFPVVICSAMGKNFNT
                     QDFKKSILYHHNLGYGPQFVDYERRISFPSIAKEALKSKINIENLSEEMRVLYVAFTR
                     AKEKLIITGSTRNIQDSIKRWSNGIESLDTISQYEILKGKNFLDWIMPCVLRHRDLSN
                     LLEEVGLDAVFNVEHNSKWYGKLWNKNDILVEKKSDEEKESIEEILEKIDVNNPDSDY
                     YGEIEEKLNYIYPYEFSTRKPATISVTEIKKIQNNYEEELINTIFEQKVILKKPLFIQ
                     NEEEREKISGTERGTIVHLVMEVLDLKNVSSVNDIKSQIRGFVSKGIITEKQASIVNP
                     YKIYKFFASNIGKRMLNAEIINREKSIYAQVNMKDIYIYEKLINNDDKKLYDNESVML
                     RGIVDAYFEEDNQIVLVDYKTDFVNEENINQIIEKYKKQLDLYADIIETLTGKSVKEK
                     CIYLFGVDEAVCY"
     gene            complement(97817..101284)
                     /gene="addB"
                     /locus_tag="PC414_000097"
     CDS             complement(97817..101284)
                     /gene="addB"
                     /locus_tag="PC414_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HDF2462517.1"
                     /translation="MGLRFVLGRSGSGKSTYILDEIKKEAQKNETTSIILLVPEQYTF
                     EAENRVSKLFLGKEKDKYLRVRVLSFKTLSNIVFSQVGGLTDVNINSSGKAMMVYRAI
                     EDVSEELNVFSKSKSQSGFVSSITDMISEMKQYNISPEMLENISGELDNETLSLKLKD
                     ISKIYNSFEGKLHENYVDAQDMLTSLASKIELSSYLDGACVYIDEFTGFTPNQYNVIK
                     SILNKSKSVNISLTVDDINYIGYSKSDMFSRTKFTYSKLTQLCNEEGIKILPQVNLNT
                     GVIKRFEKVKELQHLERFYNAYPYKIYSNPTENIKIKEFNNLYSEVEEIAREIVHLVR
                     DKNVRYRDITIATRDLNRYDFLVHSIFNEYNIPNFIDKKREAKSNPIVILIISALEMK
                     NRRYGYETMFRYLKSGLIGIDNDDINLLENYVLANGIKGKKWFDEKWDYRITQSLSGQ
                     ESEFELELKEKINEIKNRVLEPIVILQEKLRGKNRVKEICRYIYEFLLDINMPETIES
                     LIVNFKDKGELDVANQYSQVWDIVVDILDQMVELMGDEIISLEKFIKLITLGFDEYEL
                     GLVPPSIDQVLVSSVDRMKNPDTKYLYLVGTTDGVFPLITKDSGILSDNDRESLGNKG
                     IEVDIDSKTRSFEEQFLVYKALTSTSKNLTITYPISDHEGKTLRPSIIISRLKKIFPN
                     IENKSYLVEENKSTDKDILKKITVKSPTFNELINVIKNYDSDGYNTEEINSIWLDIYR
                     YYLKDEIYSSITKKVIKGLSYTNQVHKIEEKKIRSLYKSNSLSISRLEKYAECPFAYF
                     IQYGLKAKKRKEYSFTPPDLGTFIHNILDRFSKELLQDNLTWRDIDEKYIELKIGIIV
                     DEIILKIPGYILNSSERYKYLAYRLKNMLTTAITIISQQIKQGSFEPIDYEVKFGNNG
                     KYPPIKMVLENGQEVSLIGQIDRVDEFEEGENKYIRIIDYKSGNKSISLTEIYYGLQL
                     QLLVYLDAILESAKDEDININPAAILYCRINNPIAKFNEDKDDEEIQEAILKELRMKG
                     LVVKDSHIVKEMDKSLIDGERKNSLVIPVGLTKDGNVGKSTSAISYEDFKLLRKYVRH
                     AIKDLCEEMLSGEIRIAPYKHKDGTSCDFCDYSAICQFDSTIKDNKYKNLNNKSNEEI
                     IKMMKGDVN"
     gene            complement(101274..102104)
                     /locus_tag="PC414_000098"
     CDS             complement(101274..102104)
                     /locus_tag="PC414_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HDF2462518.1"
                     /translation="MNDKLKYFSYSQNSINTYKSCPLKFKYKYIDRINWKNDDVGSRE
                     YYETLKTGRDFHLICERYFSNIPLGIYFNEDDKNSKKFLKWMENIKKVVPIEKGKTYL
                     PEYEVRMTLNGDIIQAKYDLVVIGNNNIEIWDWKTESKKIDYKHVENRIQTIVYMFLA
                     KEVIPKVLKMDINVKDINMRYYQPEFDDLPITILYDEEKHEANRNKIQNYISMIKNTN
                     YEEHTYEDNLYNDIEKVYNKEERMCYRNKKHCKYCEFNKLCNGKEIDYSILEAEIYGT
                     "
     gene            complement(102213..104432)
                     /locus_tag="PC414_000099"
     CDS             complement(102213..104432)
                     /locus_tag="PC414_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDF2462519.1"
                     /translation="MININYREDQIPIINYDNGTMAVPAVPGAGKTFIITNLVAKLLL
                     EQKHKGGKILILTYMNSAVNNFKGRIRKILEENKIDDTNSYEVMTIHSLAVKIIKEKP
                     EVVMLSEDFNIADDLQKSIILNECISRFRFEGGERAFRWFLKEQKDERWKEITLEAWE
                     RGFFEFAGNAISELKYKGISPDNLEEILSRGHKGMLKIILPIYKLYDRKLKQNGLLDY
                     DDILILAEKTLSLDEGLRKKFQTRYKYIFEDECQDSNEIQGNIIKIISSENKNLVRVG
                     DINQSITGTFSSSDPKFFKEFINSADFCYRMDMSNRSSKDILDLANTLVKYVTKEFRQ
                     KECRHALEDMQIKTVPNGMGYKENPNPEHYNINVKWYESWKIEIEQTARYVKGIKNKY
                     PDKSIGILVPFNEQVTQVAKELRENNLTFEELGPNSLNKRKVINNIAYIIDFMLHCDD
                     VEKLIVVLNKVFIDTDNEEEIKYFLEELKNYSTEEIIYNFEFSKLDNDLIEFKEESDI
                     YLSFKRGIDIIKQILEYPIIRLDLLVLFIGKKLNLEKEDKAVLDYISFYIKYLVSENI
                     NMDLEDVYNLLFDVKNRVFNHIIDVVYEINGYEPEPGSITLCNYHKSKGMEWDCVFLL
                     GLVEYNFPDNINQKFQSDKWYLKEKYKNPMAIIKSEVEAILKGSISTDYAHKTKIDSI
                     NEKIRLLYVGITRAKEMLVLSGSAYRDESDIGNKRKEQKPCIYLSRLNQHIIEKRSN"
     gene            complement(104425..106338)
                     /locus_tag="PC414_000100"
     CDS             complement(104425..106338)
                     /locus_tag="PC414_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2462520.1"
                     /translation="MEYLFEKSSLKEYGNEALDKYRELLDKTGNNSRCILLLVPNNNT
                     KIRYERSLRLNYSEELKITTYISFVKKELVKYWPLIIEKCDEISTKVVSPTFISNSLS
                     EYLIVQKVKEKRIQEGYFEDITGTNKSIANSIMTNINKSAFNLIDFDFIGEKIYSSKK
                     NKDSIYRFSYTQMDEIISYYINSLLSNSILDNALSIYLYNQYLLNDDFYLKNLFTEVR
                     YIIVDSLESSSNAEVDFITEALDNTLQSYIYFDYSRDYSVFNNVDIDYINEKIISKIK
                     PKEENIKKKEIRIEDLYLLPANIELNQSSQLYNEMLDLISEKVISLIEEDVSPRDIAI
                     ISPINNSILEHQIRDSLIEKNIDVFSTKKDKKAVDYPYGNALVVATCIFYGYLDFIKD
                     DEYLSFLETLLEINRIKAFKIFNETRHLSIDNIDEHSQYREILQYIEEKKKSDIKIHE
                     FLTQFYIDKMLNLKEGKKNVGLCKKIIAESESFSESLSLLGMKKEKIFIEALKSTIND
                     YYSVVDIEELKSKDKIVITTPYSYISSNIDRSIQIWVDIGSNAWNMKIEKDISNLIVL
                     RKSFEEKKIYTNEMEEYYKKYYLYNTVYNLLLNAKKIYAFKSEYAINGYIQESILYSI
                     LLKISHKGDKNYD"
     gene            complement(106580..108592)
                     /locus_tag="PC414_000101"
     CDS             complement(106580..108592)
                     /locus_tag="PC414_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2462521.1"
                     /translation="MRRNTKLLTTGILSMAIVAPTMAFATESNAMENNADLNINLEKK
                     SIVLGSKSKVSVKFKEKPDADSITLKYKCYDMPLNTTLNYNQSTGAYEGTINYNQDPE
                     YLNVWELQGITINSKNNPKTLNRQDLEKLGLNLKDYNVTQECIIEDITSRKDVNKYLR
                     KTSSPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPI
                     LLVEKNNVPNSVKSELKRLNPKDIIIIGDENAISKTTANQIKSTVNASQTRLNGSNRY
                     ETSLLIAKEIDKNHDVEKVYITNANGGEVDALTIAAKAGQDKQPIILTDKDSITDNTY
                     KWLKSEDLQNAYFIGGPQMISTNVINKVNGITKDSVTNNRVYGADRHETNANVIKKFY
                     TDDELEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYHKDNLEAKSANKVY
                     KIGGGLTSKVMSSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGSAPGNSSGGMIEKD
                     YNLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLKPDLFTSIHYNGSTNK
                     QGHGVEVFYKLKDKNGGTTKTVATNILNRILEKFKLTNRGIKTRVLPSDSTKDYLYVL
                     RSNDMPAVLVECAFLDNENDMSLINSSAKVKEMGTQIGKGIEDSLK"
     gene            complement(109097..111115)
                     /locus_tag="PC414_000102"
     CDS             complement(109097..111115)
                     /locus_tag="PC414_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2462522.1"
                     /translation="MRRNTKLLTTGILSMAIVAPTMAFATESNAMENNADLNINLEKK
                     SIVLGSTSKVSVKFKEKPDADSITLKYKCYDMPLDTTLNYNQSTESYEGTINYNKDPE
                     YLNVWELQGITINSKNNPKTLNKQELEKMGLNLKDYNVTQECIIEDITSRKDVNKYLR
                     KTSAPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPI
                     LLVEKNNVPNSVKSELKRLNPRDVIIIGDENAISKTTANQIKSTVNASQTRLKGSNRY
                     ETSLLIAKEIDKNHDVEKVYITNANGGEVDALTIAAKAGQDKQPIILTDKNSITDNTY
                     KWLKSEDLQNAYFIGGPQMISTNVINKVNDITKDNVTNNRVYGADRHETNANVIKKFY
                     TDDELEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYHKENLEAKSANKVY
                     KIGGGLTSKVMSSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGSDTGTTGKPLGGIR
                     EKDYTLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLRPDLFTSIHYNAS
                     DTTGNGVEVFYKLKDKDGGTTKTVATNILNRILEKFNLKNRGAKTRTLSTDPTKDYLY
                     VLRNNDMPAVLVECAFLDNEKDMSLLNTSNKVKEMGTQIGKGIEDSLK"
BASE COUNT        34839 a        20063 c        11652 g        44626 t
ORIGIN      
        1 ttctattaaa tttttattga aatacaattt ttttacaaag tatgatatat tataaacata
       61 aaactattgt tttacatgac ttatcatgac atcttttgtt tcattcacct caaaatgggg
      121 tatgttactt atataatgat aaattttagg agggttaaaa atgagtaaag tttttgaagt
      181 aaaaaaagat atctacttta ctggagtagt agatgaaggt ttaaaagtat tcgacataat
      241 aatggaaact gagtttggga caacttacaa ctcttattta atcaaggacg aaaaaacggt
      301 tttatttgac actgtaaagg caaattttaa agatgagttt ttaagtaatt tatcagaggt
      361 tactgatata gctaaaattg actatgttgt aattcatcac acagaaccag accatgcagg
      421 tagtttaaaa tacttattag atataaatcc taatatagaa gtttattgta ctaaagctgc
      481 taaactatac ttagatggtc aaataaatag accttttaac tgtcatgtaa taaaagatgg
      541 tgaaatttta aatatcggta aaagaaatct tagatttata actgctccat tcttacattg
      601 ggttgataca atgtttactt acattgagga ggacaagact cttttaactt gtgatgcatt
      661 tggttgtcac tttgcaagtg ttgatgcaga ggtagtgaat agtgaagatt atctaaaatc
      721 agctaaacac tactatgatt gtatagttaa gccatttgct aaacatgttt taagtgctgt
      781 agataaggtg gtaggactaa acatagaatt tgatactata ttgacttctc atggtccaat
      841 gcttactaaa gacccaatgg ctgctgttaa aagatatgtt gagtggtcta cagaagctgt
      901 taatacaact aatcaaaatc aagtttctat attctactta tcagcttata gtaatacttt
      961 agagatggct aaaaagataa aagaaggact tgataaagaa ggtgcaaagg ctgaattata
     1021 tgatttagaa gatatgacat taacagaaat gcacgataca ttagtagtgt caaaagtaat
     1081 attactaggc tctccaacta taaacaaaac tatggttaaa cctatgtggg atttattctc
     1141 tgttatagac ccaatggcta accaaggcaa gatagcagga gtatttggtt catttggttg
     1201 gagtggtgaa ggtataacta tggctgagac tttacttaaa tctatgtcat tcaaaatgcc
     1261 tgtagaatct cttaagaaaa aattcttccc tagtgaagag acattaaaag agtgtatggc
     1321 atttggtgca gaatttgcaa aattagttaa atagccatta atcaaacata atattattta
     1381 aaataaatga ttttatattt aaaaataaat taaaatggag ttgtctcaaa attaattttt
     1441 ctaatctaat tttaagttca actccttttt tattttccaa aatgactaat ataataataa
     1501 atgtaccctt tcgcacaggg tgcattattt atagtctgtt ctatttgtat actagtatac
     1561 ttgtattaat tcacgtatct agtatatgct actagcccta ttccagaatt agatgttgtt
     1621 tcaccaattt ctactttaat aattatatta tttttttaca cttttttgat aattctgctg
     1681 cagaaaggtt cttaccagta aaatctaaag aaaatgcctt atcatactca aaaggataac
     1741 taacaggttt gctctctggt gctaatgcaa ccataccata tggtgtattc ataaacccta
     1801 ttacagtaac tgtatgcttt gatgggtcta aatttgaatt tttgcttgta aacttaattg
     1861 tatttattga tgagttgatt aaactacacg gaactccctc atacatttca atttttggac
     1921 gtagattatt tccataaact tcatcaagta ttgccccttg ttgatctgct atcatagtgt
     1981 gattcccagg tccaactggc gcagaaggct tagctggcgt aggtttctta acagttggcg
     2041 ttggattctg tgtctttact acttcgccaa cagctggata tgctttcttg tcaaatggta
     2101 atgggtgagt aagactcttg tatatgatgt ctgctcctgc tgatttgttg gcatcaatat
     2161 ctacatgata caaacgtcca tcctcaaaac gaataagatt ccactgatgg tcatttgttg
     2221 tactatgtac ataataacat tcaattccca tagcatcaca taaagctttc aagctcctac
     2281 acattccaga acaagcacct tttttagtaa ccaatgtata agcagtattg gctccctctg
     2341 ctgcgttcca gttaacatat gttgtgttgt ttttaatgtg attatgtata gatacaatcc
     2401 actatggaga aatataaaag aaaagaatat aacaaacaat aaaaaataag atgattcaaa
     2461 ttttattttg aaacatctta tttactctac aaattaaaat tatttggtga attatttaat
     2521 tattattttt tattaaagaa attttaaatc ttaatataaa atacgctatt atacataata
     2581 atccaaatac gaatgctact ggaattaaca tctccgattc aaaataaaag aaatattcta
     2641 agaaaatatc tggatttact atattcccat tcttgtctaa aaaatctttt ccattatatc
     2701 ctaaatgctg tataaaatta cttatgttat tataatctgc ttccctctca tataccatat
     2761 tagcattcaa tccagaagaa cttaaattag tgtttccatc tttcatatct attacatata
     2821 gtctaagagc tttgacttta ctatacttat ctttatcaag tattttttca agttctaact
     2881 tatattcacc aatctttgtc atatcatgtc tttctgccaa ttgctttatt tctccatcaa
     2941 gttcttttcc taaattatac atattatgtt gtaaactatt attgtaatac cacataaatc
     3001 cagaaattaa aaatgcaatt atacttatgt aacctaatac ttttttaaac cccattacaa
     3061 ttgatttatg cctatccaat gataaagata gttcttttat tatattacat aattcatact
     3121 gcatttcatc accatcatca aatctttcaa ttgctttttt acatgcttct tcttcatcta
     3181 atccttctaa tttaaattca tttgcagatt ctataagatg gcatctcatt tcctgtttta
     3241 attctatagt acttttattg tcacattttt tatatagttt atctaaatat tcatctatta
     3301 gtttcataaa ttaatccacc tctcccaaaa attgattcat aaccttttta ataaatctcc
     3361 attcttgttt ctttatttct aaaaaatctt taccttcatt agttaaatta taatattttc
     3421 ttcttccacc tgaactttgt tcattgctat aataagattt aattaaattt ttagattcca
     3481 ttcttttaag tgcaagatac attgttcctt cttttaattc aaactcactt ctttctctta
     3541 cttgctttgc aatttcatat ccataacaat ctttcttttc taatatagaa acaattaata
     3601 tatcgatata accttttaac acttctttat ttaactgcat tatgtaccac cgccttttca
     3661 cctactactc tataatgtat agtacttata tttactataa tattacatag tactctataa
     3721 tacaaggttt atattctaca atagacttaa aaataataaa tagctgtata ttaatattta
     3781 atctataaat actaatatac agctatttat caaattttaa aaataaattt ttgttttatt
     3841 aatgttgtca taacaacatt tacttggttg aaatagtatt acctataagt ttaaatatat
     3901 ataataatag acttgattct atttcatatt attaaaatag agtaatagtt taaattctac
     3961 tactctatct ataatctatt tcctaattat ttaatctcaa atttagctct tcatcattta
     4021 aatctttcat aagatttcta caggtactag atttattcca acagaaaaac tagggcatta
     4081 aacacttata tatctgtttc tttagactgt aatattaata ttttttaaat acgttgactt
     4141 ttgctttttc taacttctct ccatgatagt tctcctctat caagagcttt tataaatatt
     4201 tctgctgaag ctagatttgt agctattgga acatgataaa catcacaaag tcttattaac
     4261 gcttgtatat ctgtttcatg agcctgagat gtcaatgggt ctcttaaaaa aataactaaa
     4321 tctatttctt gtgttactat taaagaacct atttgctggt ctccacctaa aggaccagat
     4381 gctaatctat ttatatttaa ttcagtttca tccattattc tttttcctgt agttccagtt
     4441 gcatatagtc catacttttt taaaattgat tcataaccta tacaaaatcc gaccatagtg
     4501 tttttcattt ggtcatgtgc tactaatgct atattcatat atctaatctc ctcaatttat
     4561 gtttagatgt atttaaattt agaaatttat tttctttctt ctcatacaca catttagtac
     4621 taatcctaag tttgctaaag atgtcaataa cgaacttcct ccataactta caaaaggtaa
     4681 tgttactcca gttactggta ttaacgcaac cgtcatacct atattttgga ttatctggta
     4741 accaaacatt cccataacac caaccactat gagggttcca taaaaatctt ttgcatctct
     4801 tgcaattgct aaaagcctta ataggaatat cataaataag attatcaaca ctgccattcc
     4861 tattacacca agttcttcac ctacaacagc aaatataaaa tcactatctt gtacaggtaa
     4921 aaaatcttct tgattttgac ttccattgta aagtccttta ccagttacgc ctccagaacc
     4981 aattgcaatc agagattgca taacttgata gtttcctttt agagttacat cttctggatt
     5041 tagaaaagct tctattctta ccttttgatg gtctgccatc attaaataca tcagtggcat
     5101 tgacactaat agtatgatta ttcctctttt tatcaacttt gaactaagac ctgctgtgaa
     5161 cagcattgca aatatcatac aacaaaatac aattcctgtt cccaagtctg gttgtgcaat
     5221 caataatccg ataaatggaa gagaataaac tacaacaggc ataacttcct ttaacgtatt
     5281 taatttatcc tttttggatt caagtatctt tgcataactt agtacaaatg ttaatttgac
     5341 tatttcagat gtctgcaagt caagtggtcc taagttaatc cacgacctag ctccaccttt
     5401 tactgtccca attccaggta ataaaactat ggctaacaaa ataagactta ttatataaag
     5461 cgctttataa tacctgccta gaaggttata gtcaaaaaat aatatgacta taatcattcc
     5521 tataccaagt acaaaagcta atccttgttt aatcaattga ttatatgaac ctgttgaatt
     5581 agcatgtgtt gcactactaa gtataacaag cccaaaaata aagatagcta atactgtaac
     5641 tataagtttc cagtctaact gttttatcaa cttaattgtc gacttataat taatattgat
     5701 ttttttcaaa gaatcaccgt ctttctatct ttaaacatat aattaaataa aaggggcata
     5761 taccaataag gatatccccc ttttaagcac taattacata catctatctt ttatattctt
     5821 tagtggtata ttagcaacta atgcagatac aggtttgtca tcatgctcac ctctgcattt
     5881 agacattttt atttcaagac catcatcttc tatttctgca taattagcta ttactttcaa
     5941 aatgtctgtt tttatcatct ctaaaagttg tggtgaacaa tctactctat catgaactaa
     6001 aactagcttt aacctctctt tagcaacaga tttactagtt ttagcctcgt tagaaaaaac
     6061 tctaaataaa tctaacacag atgtatcctc ctttgctact tagccatgct aaacattttt
     6121 ttaagtctac caaagaatcc atcttcaact tcaagatcaa gtagaggaac ttcttcattt
     6181 agtatccttt ttgcgatatt tttgtatgct tgaccagcaa gtgacttaga atcaagtata
     6241 gctggctctc ctttatttgt tgatataatt atgctttcat catcaggaac aagacctaac
     6301 aaatctattg ctaatatttc tattatatct tgtttatcca tcatgtctcc acgcttaacc
     6361 atatcatttc taattctatt tataactaat cttatctctt ttatttcatt tgcttctaat
     6421 agacctatta ttctatcagc atctcttact gctgatattt ctggattagt aactacaata
     6481 gctctatctg ccccagctac tgcattttta aagccttgct caatacctgc tggacaatct
     6541 attattatat actcgaaaga ttcttttagt ttctcacaca ggtcaatcat ctgctcaact
     6601 gagacagcat ttttatctct agtttgtgct gctggtaata aatatagatt atcaaatctt
     6661 ttgtctttta ttagagcttg cttaagccta caagttcctt caactacatc tactatgtca
     6721 taaactattc gattttcaag acccattact acatctaagt ttctaagtcc aatatctgca
     6781 tccacaacta ctgttttttt attttctagg cttagcgcag ttcctaagtt tgctgcggta
     6841 gtagtttttc caaccccacc tttaccagat gttataacta taacttcgct cataatacta
     6901 ccccctattt ttctttctat ttgtctaact ttgacaaata gctttcgatt acaattcttc
     6961 cattacttac aaaggctatt tctgggctaa tttctgattc actttcataa ttttcttcat
     7021 catctggagc ttctgcaata ttatctgcta tttgtaatat tttaggatta agatttccag
     7081 caacaacata tgcaaattca ttgccttctg cacctgcatg tacaaaacct tctattttac
     7141 ccattacaaa aacattacct cctgctacaa ccttagcacc agcatttata tctcccataa
     7201 caactgtatt tgattttgaa aggacttttg aaccaggttt catatcattc aatataacta
     7261 catcaccttc aaattcaata ttttcaccag atcttagggt attgacatat tttgttggaa
     7321 aattactatt ttcttcaatt ttttgatctt caacaaattc cacatcaaat ctagaagtaa
     7381 ttccttcttt aagttctaga atctccatgt cagttaaaca atcactatta attttactaa
     7441 ttttagcccc attaaaaaag ccaacatatg cttccaattt gtttattatt ttttcctgta
     7501 tttcctcaaa tggagcttct ctttttatat ttactattat ccctctttta ttacccttga
     7561 actctactag ttcttgacta caaatttctc ttaaagacat aacaacctcc agagtgatat
     7621 atcatttata ttcttcaaat acgctttaaa cccttttatt ttttctatat aaagctaaag
     7681 tttatacata gattgagcta tctattaatt tgtgactcga aatttatatt ttcgtcagaa
     7741 gaattgttaa catcattcgt cttgttgtca tttttactat tagttggctc taatcccata
     7801 taagttccaa taacttctct agttggtaaa agtgagaata cacttgaatc accttgagga
     7861 attacacata caacagctat ttctgggtca tctgctggag caaaagcaac agtccacgta
     7921 aaagaaccat aatcttgttt aaatctatct atttggtcat ctgttatttt tggatttaat
     7981 tcttttatag cttttcttag atatgaagaa ttcaccttat cagtgtcctc taatctaact
     8041 tttactccat cttctaattc ttcctctaat ttctttcttt cttcgtcttt caaatcttta
     8101 ttttctaatt tcttttttat ttccttttcc ttagctaatg aaagctcttt ttctttttct
     8161 gctttcatct tatcagctag tttaatcgca tcatttaggt tgacattata agatgacata
     8221 tgacttttta aatattcata ctcgttatct gtaggtattt tcccactttt ctcagctgta
     8281 ccagtttttg ctgctacatc tattggaaaa tcagcaaaag cactcttcgc tgtaccttgt
     8341 cttgcaacca atttcatacc ttttgttaat tcttttaagt tatctggatt tttaaaaggt
     8401 attttttcga ctttcttctg gtcattaatt ttaactgatg aataatcact tgaaacagct
     8461 ctatcaacta cagataattc tactaaatta cctccatttg ctatagcagc tacatatctt
     8521 gatatttgag ctggtgtata agcattttca ccttgaccta tagctaagtt aaatgtatct
     8581 gcagtactcc attttgcaaa gttaaaataa ctaaatactg ctaaatctgc tacttcttca
     8641 attctatctt ctttaacatc caattttttt agcctattca ttgtttcaac tctaccagga
     8701 gttttctttt ctgctgccca gcttacaatc tcattgattc ttttctcata ttctttagga
     8761 tttttttctc tagttatatc aacaaaatca tttgccattt ctctttctaa atttgacttt
     8821 agaagagctt gtgttgattt caatttatct tctgtgctag gaactttacc tgctctttct
     8881 tctacttcat cttgaagccc tgtattttgg tcaagcccaa ataattttgc atactcaagt
     8941 attttatttg cattcatatc tataccaatg ctttttccat ctggccatgt tttacctgta
     9001 ccaattgttg ccatatatat attacaagac tcttgtattg ctttatataa gtttgtcata
     9061 ccatggtttc ctctaccatg gttccataca tagtcaccaa aagatttttt acctaacatt
     9121 ataaccccag ggtcatttat agtaaagttt ggatccagac cattttctaa cgctgccatt
     9181 gaagttacca tcttaaatga agaacctggc tgaaattccc cttgtgtaac caagtttaaa
     9241 agtggacttc ctgctagtaa gtcatttgga ttttttggtt gaagtgcttt ataatcctct
     9301 gtagaaatac cttttgcaaa tttatttggg tcataattag gataacttga tgaagctaat
     9361 acttctccat ttttaacatc aatagctata agagctgctg actgtgcttt tcctgcatat
     9421 gcactaattg gtttgtcccc aaatttactt ttaaaagtac ctcctttaga agcaacttcg
     9481 attatctgtt ttaatgcatt gtctgaaact tcttgtaaat ctttatctag tgtaagatac
     9541 acagtatctc ctgatttagg ttttttagat tcaatttctt tactgattct acccaatgca
     9601 tcaactttaa ccattttata accatcagtt cctcttaatc tactttcatt actcttttct
     9661 actcctgcaa gacctaccat atctccagtt tcataacctt tttgaaggta tgattctatt
     9721 tgagtggatg gcatttttcc tacatatcct aacatatgag aagctaatga cccattagga
     9781 taatatctta ctggttctac tgctattgaa acaccaacaa ggtccatagc actttcttca
     9841 atttgagcaa tcgtagtttc tcctacatcc ttagcaatag ttactggatt atattgagaa
     9901 tatccttttg attttatcaa atcccttacg accattattt ttcttgcatc ttcatcactc
     9961 aaacttttat caatttctaa tgcatcacta tttcttactt tttcaaacgc ttctttggca
    10021 ctcagtttag tttcttttat cttataacta gccaaccaat ctctcttatc tctttctgct
    10081 gtgaacatcc atttactaac taaaataggt ggataatatc cattttcatt tagttttgcc
    10141 tgtaatcttg tcgcatccaa tcttttatct tcttgagata atattccagc tgatataagt
    10201 ttgtccacca agtaataaaa actttcctta gcattataat catttggaat accattttct
    10261 gatttatatt ccctaatatc tctgtcatat gtataatagt actttccatt ttctacatat
    10321 ataggaaact catctacata ttcttcacca tttctctcca gcaaatttat aagttttaat
    10381 gatatttcat ttgctcttga atgcttattt gcatctttct tattaatatc gttacctgaa
    10441 acttgaacag taaataagtt tttatttcct gccaacagtc ttccatatct atcttttatt
    10501 tctcctcttg gagcttctat tgccaacttt ttatatgttt tattctcagc taactcattg
    10561 taatattcat atttaaatgt agtcatatag attattttta ctaaaatagc taaaaatatc
    10621 acgcccatga ctatcttaat tatttttagt ctatcaactt gtttattttc ttccatgaaa
    10681 tcaccaacta atcttcttta agttttaaaa ctgctctttt agataagcga tatattacta
    10741 aaccaattaa actattcact attggtgcta ctagtagacc ttttacaaaa agtgttgata
    10801 tgttgtagct ttgataaatt atactagatg tacctatatt gacaattgaa tcaattaaac
    10861 tgcctataaa aattaaaacg aaagtaataa tataactacc tttatatagc tgatctctca
    10921 tgtatccaat tgcatatgca ataataaaca aaattaatgc atttacacca aaaattccac
    10981 caacggttat gtctttaata agccctatta ttgctgctat aatcccacct tctaattcat
    11041 ctaaataaag agagattatg gttacatata taagaactaa attaaagctt atgccaaaaa
    11101 tatctatata atttgttata ctattttcta tagtcataag cacaatcccc aacagacaaa
    11161 gtaaaacttt tttcattgtt tttatcccct ttatcctata tttcttggtt ctatgactac
    11221 aacgtcattt atattcttaa aatcaacata aggttttaca actacatatt ttaatgactt
    11281 atctttatca tcaaccactt tttcaacttc gccaattggt ataccatctg ggaagaatcc
    11341 aagacctgat gttgttatta tatctccttg aattacatta tatgatgagt caaacatata
    11401 tccttgcaaa tatcctttac tattataact atctttatta tctaaagtag tattttgagt
    11461 aattgtacct ttagcattgg catcctttgc tagtttaaag cttacagatg ccttagaatc
    11521 caatagtgaa attgccttac tataattgtt ggaaacttca taaactatgc caactaatcc
    11581 actaccattc ataactatac tttctttttt tacacctgaa ttttttcctg cacctatgac
    11641 aaaactctca taccagtttc catcattttt accaacaaca cttgctgaaa tacttgtcgc
    11701 cttatactct tcttgtacaa agttcagtgt cttttttaac tcttctagcg attctgtttt
    11761 atctagtttg gcatttaaag ctatcatatt ttttttaagt ttttcatttt cttcttctaa
    11821 tttttttacc tttttagaat tatctttaaa atttgctaca tctttaaaac catttttgat
    11881 aaaagaaaat ccatcattta ttcccttacc tacacttgta atagcatctg aagctactcc
    11941 taaatttact ggactaccac tagaaaattt accaattgat atacccacaa ttccaattaa
    12001 agtgatagca actacgcccg ttgctatcac tttaacatta atcctttttt tctcattttt
    12061 gttcttgtca aatctcaagg ccatcaccaa cttctaattc ttagtattca tcattaatac
    12121 tttttcaaag atttcttggt cttcaacaga tttacctgtt ccaagtgcaa cacaatcaag
    12181 aggtgtttct gctatttgta cagacatacc agtttcctgt gtaataagct tatcaagacc
    12241 tctaagcaaa gcgcctcctc ctgttagcat tattccgttt tccattatat ctgaggctaa
    12301 ttctggtgga gttctttcta gtgtagattt tattgcatct actattgaac ttacaggttc
    12361 ttttaatgct tctctaactt ctgtagagca tatttctatt gttttaggta atcctgagat
    12421 taaatctcta cctcttattt gcatatttat ttcttcatca tctttaaatg tagaacctat
    12481 gttaattttt acattttcag cagttctttc acctatcata agattatatt ctttttttac
    12541 ataagccact atagactcgt caaattcgtc tccacctatt cttatagatt tagcagtaac
    12601 gattcctcct aaagatatta cagctatttc tgaagtacct ccaccaatat caactaccat
    12661 attaccttct ggttcttcaa cttttaatcc agcccctata gccgctgcca ttggctcttc
    12721 tataaggaat gcatctttag ctcctgcttg tcttgcagcc tcttctatag ctctcttttc
    12781 tacttctgta actccaaaag gaacacatat agctattctt ggacttacaa cacctttttt
    12841 atcagccgct ttttgtataa aatagctaat cattgattga gttatgtcaa aatcagctat
    12901 tactccatct ttcataggtc ttatagctac tatatttcct ggtgttctac ctatcatttt
    12961 tttggcttct tcacctactg ctaatacctc tttactatca tctcttattg cgacaactga
    13021 tggttctcta acaacaatac cttgtccttt tatgtaaact aatgtatttg cagtccctaa
    13081 atctattccc atatcctttg tcatcttatt aaaactaaaa aaaccttttt tttctttctt
    13141 tttttcctta gccatgattt tactccttcc tactattaag aatcatgttt aaaacattct
    13201 tctattattt tatcacatat aaagtcaaat tatcatattt tctttaaagc taaagtataa
    13261 tccatctcca ataattatat ggtctattat ttctattcca attatttctc cacatttaat
    13321 aagtcttgat gtgatatttt tatcttctct tgatggttct acacttcctg aagggtgatt
    13381 atgcatcact attatcttac ttgcacttct tcttatagct tctttaaaaa cctctcttgg
    13441 atggactaat gacgagctta aagtccctat agatacatct acatcagata ttatctcatt
    13501 ttttgtgttt aaaagaaccg ctttaaaaac ctcttttttt aaatacctta ggctatccat
    13561 ataatacctc tgtatatccc aaggattcct tattttatac tttataggtt taaaactagc
    13621 tactctagag cctagttcta aagctgcttt aatttgggtt gattttgata acccaattcc
    13681 atctatatta cacagctcct ctattgtcat atcttctaaa tgtctaatac cctgaatatc
    13741 tctatttatt atatagttgg ctaattcaat agcattttta ttcttgttgc cagttcttaa
    13801 aagtatagcc aacaactcag catttgataa gctttttacg cctttggcaa gcatcttttc
    13861 tctgggtctt tcctctggtg ccatttcttt taccttaatg gttgaattaa atgacttttt
    13921 cagataaatc acactccata aaaaagattt atactaaaat atttttttaa taggtcgcct
    13981 aaccttgaaa ttggaagacc aactatatta aaataatctc ctctaatttc ttcaactaac
    14041 aaagctccat aaccttgtat accataggct cctgctttgt caaatgactc attagttttc
    14101 agataatcct caatatcctg ctctgataac gttttaaatt tcacattgct gattacatag
    14161 tcaattacct ttttatcttc gcacaagttt attaagctaa tacctgttat gacttgatgc
    14221 tctcttccag atagtctttt taacatatct cttgcacaag actcatcttt tggttttcct
    14281 aaaatagcat tgtctaaaac aacaactgta tcagctccaa taacaacatc ttcattatgt
    14341 tcagatgcta cagacatact tttttcaaaa gctaatctca taactaaatg ttttggtgtt
    14401 tcaccttcca atataatctc atcaatttca tttttaatta tatcaaatct tacattcgta
    14461 ttctctagaa tttctttcct tcttggagag gctgatgcca agattatatt catttattca
    14521 cctcatttgt acgtaatttg tacctattta gtctatcact atattgtaaa aaattcaaca
    14581 gaaatgtcga accttttttg ttacactatt gttatattta ttaccattag aataaatata
    14641 cataaaatag aggtatggaa attattccat acctctgttt tatgtactca ttagagatat
    14701 ggaaattatt ccatatcctt attttgtcca tattacaaaa ttttaatagc tttctttgga
    14761 catttttcca tacataaatg gcatcctaca catttatctg catctacctt atgtttttct
    14821 tttaattctc cctctattgc atcaaactta cattgttttt tgcacactgt acatccaaca
    14881 caaagttctt ggtcaatagt aactttcttt ttcttagtta tgtcacctga tattactttt
    14941 gtagggcatt ttgctacaca ttgcatacaa cctacacact tattagggtc tatcttagct
    15001 attttgtctt caaatattat tgcatcaaat ttacaagctt ttacacacat tccacagcca
    15061 atacatccag cagtacattt ttctttaaca gctttaccaa actctttgct attacactct
    15121 acaactactt cttgactttc tggttttgtg attattatac ccttaggaca aacttcttta
    15181 cattttccac aatttacaca cttttcttca tctattactg ctattccatc aactatagaa
    15241 atagcatcaa atttacaagc atctttacat gttccaagac ctaaacatcc aaatttacac
    15301 atcttagcac ctgagtttaa aacatttgct gctctacaat ctgatattcc ttcatactca
    15361 tatttatctt ttgcacttga acaagttcct ttacaaataa cttttgctac ttgtttctca
    15421 cctgcttcag cacttattcc cattatttct ccaacttttg caccaacatc tgctccacct
    15481 acagggcaac cattaactgg tgcattgcct tcaactatcg catttgcaag acctccacaa
    15541 cctggaaatc cacaacctcc acagttggct cctggaagta ctcctagtat agcttccact
    15601 ctctcgtcta cttctacagc aaacttttta gatgcaaaat ctagtactat cccgaagatt
    15661 aagcccataa ttcctaaaac taatacagct gtaagtatca ccattttcac ccctcttttt
    15721 tatagcttta taagtcctgt aaatcctaag aaagctattg acattaaacc tgctgatata
    15781 agcgttatag ggaaaccttt aaatgcttct ggtatatcag ctaattctag tctttctctt
    15841 atacctgcga atattactat tgctaaagta aatccagctc cggctccaaa tccgtttatt
    15901 attgtttcta ctaaattata acttttttga acattaacta gagctatacc aagaactgca
    15961 cagtttgtag ttataagagg taaataaact cctaatgctt gatataaaga tggactcatc
    16021 ttttgaatta ccatttcaac gaactgaact atagaagcta taaccaatat aaaagctatt
    16081 gtttgtaaga atccagttcc agtttttact aataaaatct gtataaaata agttataatt
    16141 gaagctagag ttaaaacgaa cgtaactgca actcccatcc ctactgctgt atcaaccttc
    16201 ttagaaactc ccaagaaagg acatataccc aagaattgag atgttataac gttattaaca
    16261 agaacgatac ttaaaaataa aagtattaaa ttcatttatt acacctccac tatgcttttt
    16321 tacttcttaa tttattaaat cctgcaaata agaatcctaa tgttaagaat gcacctggtg
    16381 gtaatataaa tattaatact ggttggaaag atgctccaaa taatgtcatt ccaaataaac
    16441 taccatttcc aagtaattct cttactgcac ctattattgt aagtgcaaca gtaaatccaa
    16501 gaccttgacc tacaccatca actattgaag gcatagctcc tgttttgaaa gcaaaacttt
    16561 ctgcacgagc taatattaaa cagttaacaa ctattaatgg tatatatata ccaagagcct
    16621 tatcaagagc aggaacatat gcttttaaaa gcatacctac tattgtaaca aatgtagcta
    16681 taactactat aaatataggt attcttatct tatctggtgt aatttttctt attaaagata
    16741 taactaaatt tgcacatatt aaaactgctg ctgttgaaag acccatacca attccattta
    16801 ttgctgaagt tgtaacggct agtgttggac acatacctat aacttgaaca aatgttgggt
    16861 tctcatctat tagcccattt ttaaatactt tagctaaatt cataattaac cctccttatt
    16921 tattaagagc actattgaaa acatctatag cagcatttac tcctgtagta actgctgtag
    16981 atgtaatagt tgcacctgat attgctaata tttgattatc tgctgttgta cttccttttg
    17041 ctacttcaag aggctcagca gtttttcctt tgtattgacc cttaaatgca tcatccttag
    17101 cttttgctcc aagacctgct gtttctgaca tattaccaat gtcaacacct gtaacttgac
    17161 cttcagatga tatacctacc ataacttcaa tttctccacc atatccactt ggtttagctt
    17221 ttaaagtata tccaaccact tcagaaccat ctaaaccttc atacacttca gctataagac
    17281 catctccaag gcctttgtaa gtatcttcct tcatttcttt aaattctttt gcttctggaa
    17341 ggactgcttt tctaagttca ttatttgctt gtatatttct ttgttctatt actggagctg
    17401 taacctgatt agtagctcca agagctaatg cagctattgc acttatagca agtaaagtac
    17461 cacctaatct caccatactg ttcattattt agccacctcc ccaaatactc tatttctaac
    17521 aaacttatct ataagtggag caagacagtt cactaatagt attgagtaag atacaccttc
    17581 tggatatcca ccatacattc ttataacagt tgttataaga ccagctaata cagcgtatat
    17641 tatttgacct tttttagtca taggcgaagt tgtataatcc gtaagcatga agaatccacc
    17701 taacataagt cctccagcaa acagttgata tattgcgaaa gttatattaa atccacctaa
    17761 aaggaatgtt aatataaata cagttgctat gtaaaatact ggtataacat aatttattat
    17821 gcctttatac attaggtata ctccacctaa taataataat attgctgatg tctcacctat
    17881 acatccacca atattcccta tcatcatgtc agcaagacta attccattgc tagaaaggtc
    17941 tgccagtcct gctgctccat ttttcaaaaa gcttaatgga gttgctgtag taactgcatc
    18001 taaattttta cctatccagt taacacctgg ttgagtccaa gcagtcattg ctactgggaa
    18061 tgatgctaat aaaaatgctc ttgcagcaag tgctggattg acaaagttac atccaattcc
    18121 accaaatacc attttaacta ctaatattgc aaatattcct cctactagac acatccacca
    18181 aggaagtgat gctggtacgt taaatgctaa taataatcct gttacaacag ctgaaaaatc
    18241 tcctatagta cttttttgct tcgtaaattt ttgatataaa aattcagtac ctactgtacc
    18301 aagtatacaa aaaaacatag cactcaaagc attaagtcta aagaagtata ctcctgctac
    18361 tgctgctgga aggagtgcta taataacttg tttcattata tatgaagtat cttcattact
    18421 tctcacatga ggagaagatg atactatcaa cttattttcc atcgtttttc ccccttaact
    18481 ttctttattt ctgagcagct tgctttcttc tctttgctcc tatttctctc tttgctgatc
    18541 ttatagattg aagtaatggt cttcttgctg gacaaataaa tgagcaagac ccacattcta
    18601 tacaatccat tgctctatga tattcagctg tgtcatagtc atcttttaaa gaatatgcac
    18661 ttatgaaaag tggttgtaag aatgatggac atacatctgt acatcttcca catcttaaac
    18721 agttttgaat atctggagta cgtgattctt cctcatccaa acataaaatt cctgaagaac
    18781 ctttgttagt cgctatttca gtagtatatt gagctattcc catcataggt ccacccatta
    18841 taaccttacc aatcttctta tcttctttaa atccaccaca ttgttctatt atttctgaaa
    18901 ctaatgttcc aacttttgtt attaggttct ttggctcttt gatgcaacta cctgttattg
    18961 tagtgattct ttcaaccaaa ggcatacctg ttttaataga cttagctatc tgtgctgctg
    19021 tggcaacatt gtcaacaact gcacctgctg ctattggtaa tccaccagat ggaacttctt
    19081 tgcctgtaca cgcataaata agttgtttct ctgctccttg tggatatttc acttgaaggc
    19141 caacaacttc gatttcgcta tagtctttag ctacattttg aattgcttct atagcatctg
    19201 gtttattcgt ttcaattcct ataaatcctt ttttgacatc taataccttc attaatgctc
    19261 ttaaaccaaa cacaacatct tctggatttt caaccattaa tctgtggtct gcagttaagt
    19321 atggttcaca ttctgcacca tttaatataa ctacttctgc cttgctatct ggtggaggag
    19381 atacttttac atggttaggg aatgtagctc cacccatacc tactattcct gcctctttta
    19441 tgatacctac tatatcttct ttgctcaaat cctctaactg accttttggt tgaacactct
    19501 catgtaattc ttccttaaag tcattttcga taactacaca ttgagctgaa cctcctggta
    19561 cttcatgttg ttcaatagca ataactttac ctgatactga agaatgaacg ttacatgaaa
    19621 caaaaccttg ttgttcacct attttttgcc caaactttac ttcatcacca acttcaacaa
    19681 taggtttagc tggtgcccct atatgttgct gaagaggaat gtaaactatc tttggagctt
    19741 gagctttttc aagtgccttt gtgttagagt actcttttct atatggagga tgtatccctc
    19801 ccttaaaagt taagagtttc attgttttac ccccttatct ataaaaaaac attttttctg
    19861 ccttctataa gtatactata tatattaaaa aatcttaaca attgttaaaa aaatatccgt
    19921 atttttataa tttattttat aaatcaacac ctctatatta agtcaaattg aggaaaagga
    19981 ttgcttaatt aaacaatcct tttcatatag ctttctaaag tccaaatttt ctgtatttta
    20041 ttttatagaa tttataaaca tagagtactc ctgcatggat gataaaagca gacttttact
    20101 tacactatat ttttcttcat ttgctgcttt tgaagctgta tcaatttttt cattcacatt
    20161 ctttacatac tctattaaat tatctttaaa taaacgcatt tcagaataat caatcttttc
    20221 agcttctttt gatatatttt gtgatattgc ctttctattt gtcaaaatag tattatattt
    20281 ttccatatct acattatttt cagcattcga ccaaaaactt gattcttctt taaagtttgt
    20341 tattaattta tttaattctt tagatatact ctctatataa gcgtagttac ttgtatactc
    20401 taaagacaac ataggtgcat ctaagtgtga taaaaatgca tctggaaaca cctttcttac
    20461 actgcttatc tgctttctaa catcattttc atcaaaagag gaataggtct gtattttttt
    20521 tactccatcc ttctccataa ctgaaaaagg tatgtcatta ttaactaatg atgtctgtat
    20581 ttttttcaaa tcatcattat tatctattgc agcaacttga atagtataca tatcccagcc
    20641 ttctatagta gctaatttga cttcttctgg ctctttagtg ttatttaccg ctttttctgg
    20701 attagtttct tgtttatcat ctgaattggt ttgtgattta gattttttcc ctttttttat
    20761 tgaatttaat tcatcagata tatctttata agttatatct gatgaattat ttagaaatgg
    20821 tattttagcc gatacatact ccaatatctt gctatctttt atttttgtat aagaataata
    20881 gccaattaga cacaagcaag caatcgttat aacatattta cgtaaatcaa tcttatgtct
    20941 attattatta aatcctctgt atatcgtttt tcttctattc attagtttgt ccctcccaaa
    21001 attatattat taataaagta tatttttaag agtcacaatt tatgaatata aataattatt
    21061 tcttctaatt ttttaataag aaattgtact tactaaacaa atcttcttgc accaagtaat
    21121 actttagacc aatatgaact agttaaatta tccactttaa ccttattagg attagtctga
    21181 gtaccactac aatggataaa tttactattt cctacgtata tacctacatg attagttgtt
    21241 ccatcaccat cagtgtcaaa ataaactaaa tctcctggtg cataatttcc catagaaatt
    21301 gcacttccaa aattagcctg ttctctagaa actctaggaa tagaaacgcc tacagaatgt
    21361 ttaaaaacat actgtgtaaa tccagaacag tcaaatttat caggtcctga agctccccat
    21421 tgatatggta ttccaataag agtatatgca aaatctacga ttgttgaacc ttttgctgta
    21481 gatttatctg aatttccagt aaatgatggc tttgagttac tattagattg agaattttgg
    21541 ttagatgatg aatttggttt ttcttttgta tcctcattag tctcagaaat ataactggca
    21601 ctcacccatc caattgttcc actcgataac tttaccttat accaaccatt acttttagct
    21661 tttaattcaa caacatctcc accatttaat tttcctatta tagaatagct tgtcccagga
    21721 ccacttctta catttaaact cacagcagat gttaccttac cattcttgcc ttcaattgat
    21781 tcttcgctag atggtttttt atcagaatta ttagtactat ttgaattact agaattgttc
    21841 gttccaacat cttctgatgt tttagaaata tattggctac tagcccatcc agtaactcca
    21901 tttgatagtt ttattttata ccatccattg ctttgttcta gtaactttac tacatctcca
    21961 ttatttgctt taccaactaa tgaatagtta gtacctgcac cacttcttac attcagtcta
    22021 gaactcacat ttactttacc atttccacta atagttgtcc cacttgaagt tggattattt
    22081 tgacttgatg taccttcaga cgaaccactt gttgatatgt attttgcact tccccatcca
    22141 acaacagaac tcgataccct tactttatac catccattag acttttctaa aatctctaca
    22201 gtttttccct tgtataattt tgctacctta tcgcaatcag aacttggtcc acttcttata
    22261 ttcaaggcac ttgctgttac tgttcccttt tcaagagcac ttgcatttat ggagctaaca
    22321 gatactgcta ctgccccaat tcctaaagct gctatagctt ttttcactac tatcactccc
    22381 cccaacaaat ttttatagtc ataaattcat aacattatta tccattttta gtccctacaa
    22441 tttaatgata acttattttc ttattaattg taaagaaatt ataaacatat ccttaaaatt
    22501 agtgattttt aggaagtatt tcaatccaat aattatctgg gtccgctata aaataaattc
    22561 ccatactttc attttcaaaa catatgcaat ctaaatcttt atgaagtttt tttgcctcat
    22621 caaactcctc aacttctaaa gccagatgaa attcattgtc tcctaaatta tatggtctat
    22681 cccaatctct aagccaagtt aattctaatg tatgactagt aatgccatct cctaaataaa
    22741 ctaatataaa acttccatct tctgcttcct tgcgctttac ctctttaagc cctaaagctt
    22801 ttttataaaa atctaaactt ttttctaaat tagtaacatt gaaattgtta tgacaaaaat
    22861 taaacttcat tttgaaatca ctctccaatt tttatttttt ttatattata tcattattat
    22921 aaatttattt aaatcatatt aagttaaaaa tttgaatgat gatgaccaat taccaaatca
    22981 tggtaaatta tttaaaagaa tatgtcaaaa aaagaataag ctctttcaaa tgcataaaaa
    23041 atttataccc aagttatact taatcatgca aattggcaga acttattctt taaaactcta
    23101 acttttaagt ttttctttaa taatataatt tattactttt tttctaatat cttcagcatt
    23161 ataatcatct tctacttcta tatccaaaag ttttgctatt gacctcatat cttctctagt
    23221 ctttgaccta tagcttgata ttaattcatc ttttattttc tctggttcta aaagatattc
    23281 ttcatctcct cttttgaaaa caacgtattt ttgagcatat gaacttctat tgtagtatat
    23341 atatcttgat atttttctta aaatttgatt atatgaaagt cgcttgttaa tatcaagttt
    23401 taagtactct gaaaaagcaa atagctcttt ttttgattta aataatgctt ctttctttat
    23461 agttcttgat attgaagtta cactttcttg atatgcacac ttttttaaca tttttaaata
    23521 tactctatta agaccaatac tttgattgaa tttttttata ttattattaa taatcttatc
    23581 caagttacct tcaaagtatt tttttgctcc tttttgtgat acataactct caagttcttt
    23641 gtgggccata tcaagtatca ttttttttaa atcctcttta tatcccctag attccatgat
    23701 actccctccc ttagctattt atagtatatt caaaaagctc aagaaatgtg agtcattaca
    23761 aaattttttt actttttatg tatatattaa tttaaaaggg gagataatag taatgtaaaa
    23821 tcaaacatac tcaatctccc ccatttttag acctctatta ctcccccaat agaggtcttt
    23881 ttttagttta tcaatttcta ttcaattccc tttagttcaa atcctaattc tgctatcact
    23941 tcttttaatt tttcaataga tacatcatct ttcatatcta cagatgcaca cttattttct
    24001 aaatctacat ctaaaacttc aataccatct atatcttctg ttaatgcagt tttaagatgg
    24061 tttacacaat gaccacaact cataccttct actaatagtt tcttcttcat ttaattacct
    24121 ccttataccc tataagggta ttctatcata tagtttatac tttttaaata gaaattaatc
    24181 atttaatttg attcattcga ttttatatct aaattagata ttccctcata tacatcaaaa
    24241 tcatttggag taattttacc ttttaatcta ctattactta caatatagtt gtttctcaca
    24301 gctaaactta taaatgttgc attatctttt atatatgtct gtacatcttt gtatgctttt
    24361 tttgtttcaa tttcagacgt agaacttgta agtgcagaca tatatccatt taacttatca
    24421 tcagtatatc cactactcgc tataatacta cttgcatctg gtataattga tagtttccat
    24481 ccaactaatg ctaaatcata atttttagaa ttaagagcat tttccatatc cttatctgag
    24541 agctgattta ctgtactatt tattcctata gcttttaagt tttcatttat tatgtttgca
    24601 gtttttattc tctcactatt atctctgttt actattatct ttaagttcat ttcagaaaga
    24661 cttttcttta cttcctcttt ttctctattt tttttctctt cagcttcttt tttgattcta
    24721 tcttcttctg cttttttaac ttttgcttct tcttctggcg taagcttctt tgagttcttt
    24781 ttagctgatt ttttctgatt ttttacttta ttgtcatttt gatttactga gtttacattt
    24841 gcatattcta ccttagcaag acactctatc gccttatctt tattaaactc taaatccttc
    24901 atttcactat tatagtattt tgattttgaa ttaagtggaa aattaactgg agtagcatca
    24961 tccatataac cttcactaat aattctatct ttatctatag agtgtgcaat tgcttttcta
    25021 aaattaacat ctttgaaaaa aggattatta taattaaata atacacactc ataatctctt
    25081 ccttgatatt ttgttatatt aaactctttt tcttgaaact tacttaaatc atttaatgta
    25141 acattggtta tatcactatc aagagccata accatagatg tccttgcatc ttcattaggc
    25201 accataccta cttttatgtt cttcatggtt tttggaactt cttcatagta atctttgttt
    25261 acagataaaa ccattccttc tctttctgta tatgattcta ttttatattt cccatttcct
    25321 atcatattat tattcttgtc atttacatct tttgaatcta aatgattttg agatacaatt
    25381 ggaaaaatta aactatcaat tgaaaaagca tatctagctt taaattttat tgtaaaatct
    25441 ttatctgatt taatattcac agaagctata tttttagtaa attcattata tggactatct
    25501 gcactttttt gaattaaacc aatagtatag tttacatcat ttgaagtaac attttttcca
    25561 tcatgccact ttgcatcttt tagctttata tctatactca ttccatcctg agctatatta
    25621 tactccttaa ctaattgagg aattacattg tagttctcat ctatagtaaa taaactatca
    25681 taaactaaat tcattatata accaacagac ttatcagtat tagttatagg atttatagtt
    25741 tttggagtta ccattgttaa gtttatatac ttagcatcta tagaactttg actttgtaaa
    25801 cttgaatcac tactactttg ttttgtttta gtattactac aaccagctac cataagagtt
    25861 atagctaaaa cgactgttaa gacttttatt ctcttcatta gttttttcct ttcttgccat
    25921 atatcttatt ataatgttcg taattatttt ttactttttc tacatatttt gaagtttgct
    25981 taaaaggtat atcatgtaga ttttcgccat ctttactata ttcattattt tctaaccatt
    26041 ttttcacatt acctgaacca ccattatatg cagctatcac taaatctaat ttaccaaatt
    26101 ctttgtataa cttacttaaa taccaacagc caagtcttat attagtctct gggtcgaaaa
    26161 tatccacatt ttttaaattt agttcctctg ctccccaatc tctagttatg tctaatattt
    26221 gcattaatcc ttttgcttcc ttttttgaaa cagcagaact attaaactta ctttcggctt
    26281 taataacact gtaaactata ttttcatcta aattaaattc tttcgaatac ttttctacat
    26341 actctgaata ttttttagga tataaaaatt tatgtattac tttgctttcc attaatagtg
    26401 ccccaaataa gattataaaa atagatagaa tcaatacttt cttactattc acgccaattc
    26461 tccttcatgt actcaataaa tttatgtgct ttactttcta attcagttat tgttcccgaa
    26521 ttatctatta tataatcacc atatttggat ttttcttcct gactcatctg tgaatttatc
    26581 ctactaagtg cttcctgctc actgcaatta tctctctttt gtattctact aatttgtact
    26641 tcttgtttac aagtaactac taatagcttg tcaaccatat ctaaaaaacc actttctacc
    26701 aatattgccg catctaacac tactatattt tcgccttttt ttctcaattt ttctatttca
    26761 cttattattt tttctcttat tcgtggatga gtcaagttat ttaattcctt aagtttttct
    26821 tcatcactaa aaactatttt cccaagagct tttctattta gagttccatc atcatttttt
    26881 atactttgcc caaaatggac aaatactttt tccaataaca acttatcctc aaatatcttc
    26941 ctggatataa tatcagcatc cactatagga atgttaagat ttctaaatat atttgatagg
    27001 ctgctttttc cacagcctat tcctcctgta agtcctaata tcaacataaa atcacctact
    27061 ttgtctcata ccaagaacta cctacattta aatctacatc taagtgaaca tccatattta
    27121 cagcattttc catttcttct tttactattt tcttaactat atctatttca tcatcaattg
    27181 cttcaactat caactcgtcg tgaacttgta gtataagttt tgatttcaaa ttgttctctt
    27241 ccaatttttt atgaacattt atcatagcaa tttttattat atctgctgca cttccttgta
    27301 ttggtgcatt cattgcaaat ctctttcctc tatttctttc cataaaattg cttgacttta
    27361 tttctggtat atatcttctt ctgtttaaaa ttgttacagc atatccatcc tcactagcct
    27421 tttctactgt tgtgtccata aactctttta ttttatggta tttagcaaaa taactttcga
    27481 tatattcttt cgcctttgga acaggtatat ttaaatcatc agctagacca aagtcacttt
    27541 taccatatat aataccaaag tttacagctt tagctgcacc tctcaattca ctagtaacat
    27601 cttccatagg aacattaaaa acttgtgaag ctgttttggt atgaatatct tcatgatgtt
    27661 taaatgcttc tatcatattt tcatcctgac tcatatgtgc aagaactctt agctctactt
    27721 gtgagtaatc ggcatcaacc aacttacagt tatcatctgc aataaacact tttcttattt
    27781 ttctacccat ttccattttt ataggtatat tttgaagatt tggttctgta gatgaaattc
    27841 ttcctgtagt cgttatagtt tgattaaatg atgaatgtat tctattactt tctggattta
    27901 ttatatttaa aagtccttca acgtaagtag aatttaactt tactatttgt ctatactcag
    27961 ttatcttatc tattattgga tgcttatctc ttaatttttc taaaacatct gcattagttg
    28021 aatatccagt tttagttttt ttaattattg gaagctctaa tttttcaaat aatataacac
    28081 ccaattgttt tggtgaattt atattgaatg gctctcctga taattcataa atttcctttt
    28141 ctaatgtagc tattatttct ttaaattcaa cactcaattc atccaatata cttttgtcta
    28201 cttttatacc ttcatactcc atatgaccta agacttctac taaaggcatc tctacatgat
    28261 aaaaaagacc atccatactc atatccatta gacttctttc catcatagga actgtttctt
    28321 taactgtgca tattatttga cccatatact ctgctaaatc ctcaaattct aaatcctcat
    28381 agtttttaga tttaacaccc tttcctaata attcttcttt tgatttaacc ttttttgata
    28441 aatacttcat agctattgca ctacaatcat atgaggtaga tgaagttgaa tctattaagt
    28501 actcagctat tgttatatca aaatacatat tttctaagtt tatattatat ggttttaaag
    28561 atatataatc atcttttaaa ttatatccat actttttaat ttcattattt gaaaatatac
    28621 tatctaactt tgacacttca tttgaatcta cataatatat tttttctcca tctacactta
    28681 taaaaatata tattatattt ttttcaagta tattaccttg tcctctaaca gtttttaaaa
    28741 taacttgtcc ttttttgttt acttcatcta taaatttttc tatatctttt aattttaata
    28801 tttctatttt ttcttctacg tgttctgtgt catctgaatc gaccaaatca actaatcttg
    28861 ataagagact tgtaaatcca aagtatctaa atttatctaa aagttcatct ttatcataat
    28921 cgccaaaaat catactatct aaatccactt ctattggcac atctcttata attgttgcaa
    28981 gtcttttact ttgaatagct atttctttgt tttcctctat tttctttttt acactacctt
    29041 taagcttatc tgtattctct attagattct ctatactaga aaactctttt ataagcttta
    29101 tacctgtttt ttcacctatt ccaggtactc caggtatatt atcagacttg tctcccataa
    29161 gaccctttaa atcgataaat tgtgttggag tcatctcgta tttttcaatt acagaattaa
    29221 agttgtattc ttcaacttct ccaactcctt tttttgttat aagagtagtt gtattgtctg
    29281 aagcaagttg aatagcatct ttgtctccag ttactatgta taccttatat ccattttctt
    29341 ctgctttttt agaaacagta cctataatat catctgcctc atagccatct atttccaatc
    29401 tatttattct aaatttatct aaaaggtctt ttaatggctg taattgttct gccagttcat
    29461 ctggcattcc ctttcttcca gccttatact ctttatattc taaatgtcta aatgtaggtg
    29521 cttttctgtc aaatgctaca cttatatgtg ttggtttata tatatctatc attttaaata
    29581 gcattgttgt aaatccatat attgcattgg tctttagccc ttctttatta ttcatttctg
    29641 gaagagcata aaaagctcta tttattattg aatttccatc tattataatt aatttttttt
    29701 ccaaattata tcactcctaa aaattatcta tttttgtaac atatatatta tataccaata
    29761 tactgttatt taatattccc ataataataa tagtatatta aaattaaata tgttgcaaac
    29821 aatatatttt tttgctataa taatatacat gacatgcccg agtggtggaa ttggtagacg
    29881 cagtggactc aaaatccacc ggactaatac tccgtgccgg ttcgagtccg gcctcgggca
    29941 ccattaaaaa ctcctaaagc tatttattca atagattagg agttttttta tttttgattt
    30001 aaattttaag ttattttttg aaattactgt gtctttttaa aatttatata attatataaa
    30061 tttatcttct agcattaatt taaaaactaa tttattttta tataattttc tattttatat
    30121 cataacttat tacaactttt tatctaattt attgtataca acaacactac aaataaattg
    30181 aaataaaata agcaatccaa gaacaactaa aacccacttg cttattacca ttacagaaaa
    30241 tacactcaat atagaaacaa taaatataag aattacaaga acataggaca tgatttgatt
    30301 agtgatacgt ccagctttat cacgtaacat aattcgtctt tcatcattca tctctatcct
    30361 ttgatttttt atcttttctt cataatctac tgctctttca ggtttactcc aatacatata
    30421 ttgaagaatc atacatacac ctggacctaa agcagctcca gataatcccc aaataaaagc
    30481 ctcaaatgaa aactcagtaa atgtagccaa taaaacaagt aaaattccac aaataaaata
    30541 tataattcca acatataaca tattcttttt catttttact cctcctcata aataaatatt
    30601 tcttcaatac tcatatcaaa gtatctagct atcttaaagg ctaagatgat agaaggatta
    30661 tacctaccat tttctaatga acctatagtt tgtcttgata cttgcaaaat agaagcaagt
    30721 tcttcttgtt ttatgcctct ttcttttctt atttcttcta atctattttt cacatctatc
    30781 cccctttaat atctcaacta cggaaagtct actttccata attatacaac tatattccat
    30841 ttatgtaaag cttactttcc atatttacaa aaaaataaca gactattttc agcctgtaat
    30901 tttagaattt catgttgttt attaatttta atgttattta ttaattccat attatttatt
    30961 aattttaact tggtttctga attggaatcc aaacttctgt agtataatca ggagcattga
    31021 tatcgccatt gggataaagt tcaatatctg gtaaattagc atacacatac ccagaacttg
    31081 gtaaccactc agtaataatt cttttttgca attcctgtat tgccagagca tttggcattg
    31141 gagaaacaca ttcaaatact gcccatgtgc ctgatggtat taagtattca tgagtatctt
    31201 ctaatacagg cttatctgtt ggagcagcaa tataataatc taaaccctta ctatttgtac
    31261 acacactaac tccaagcaat ccacaaggtt cattatttat taactctgaa agtttatcaa
    31321 ttgttccatt ttttatgcat ttagcccaca acttaggaac tttctcaaag cactcttcta
    31381 caatcatgtt catactttct tttataccaa caatacgaaa ccctttcttt tctacaatac
    31441 gatattgcat ttctatattt ccttttacat aaatgcttaa tgtcatctta ggatatgctt
    31501 ttaaaacaac accctttgct ctagctgcag atggagaaat gttatgtata ttttgaaaag
    31561 ctctattaaa agatgttggt gaatcgtaac catacttctc agatagttct aaaattttga
    31621 tgttactgtt ctgaagttca aatgcagcct tagtcatacg tcttcgtcta atatattcag
    31681 ataatggtac ttctgcaata tatgaaaaca ttctttgaaa atgaaaggtt gaacaacatg
    31741 caatttttgc tgcttctgca taatccactc cactttcgag attttcttca atataagtaa
    31801 tagcttgatt taatttattt atccattcca tttgatatta cctccaagca aattatacag
    31861 tttaataaaa taatatacct ctctattcat gcacaaaatt gtaagcattt aaatttataa
    31921 gcatttttca atcaatacaa taaatataaa aattgtatta tacaataaaa aagttcaata
    31981 acattatatt ataaaatgtt attgaacttt ttataaaaat taatttaagt gtagttaaat
    32041 taagtattac actatcttat atattacaat atcctactat cttatagtct tctggcactt
    32101 tataatcttt gtctggagta cctaaattcc attttaaatc aaacatagtt gcacttatta
    32161 ttgttgcaaa ataaagcata actataccaa tgtctatttt ttcttcatat aaattagtat
    32221 gaccatcttt tctaacagct aaaactacag tgcctccatc aactataaat ctccatggtt
    32281 gtctatttaa tgtagatgga gctaatcttg catagtgaaa tgcatcaaga agtgctcttt
    32341 cttctaagat ttctatactt gcaacatttc cccattcgtt catataaaca acatcttcta
    32401 cacctagtct agtagacccc ttatctgtta caacttgcat atcagatttt gaataatttt
    32461 gcccagtttt tgtagcattt aatactttag cattacttac attttctcca tatccaatag
    32521 ctataattcc tgttacctct ttatctgaat taattccaag tttttctttt accttttttc
    32581 catcttcaaa agtaacccaa caagaaccaa ttccaagttc tgttacttta aagattatac
    32641 tttctcctat gtaccctgaa ttttcaatgt aattatcttt tatgtcagat aatattataa
    32701 tataacttgg agaatctatc atatttcctt tgtaccctgc tactttatct aaattattaa
    32761 aaacttcact tctattcata attcttacat caacatctat ttctggaact agtttcttac
    32821 aagattctga gtatttctta atttcactaa gatattcttc tttaatatct gtgttcttat
    32881 attctctaac agactttcta tttaatatga ttgttttata atccataatt ttatcctccc
    32941 caaactagta ttgtcttaat tatataccca ttaatatatt attttactca tttatttaat
    33001 aaataaagat tacaattttg taattattat ttattaataa tattccactt ttacattaat
    33061 aatgaccagt tgtgcaaaat ccattgccaa ccaccaaata aaaacatatt ataaaggtaa
    33121 tataaaataa caaacaaatc ttaacaaaaa attaaacttt ttttacacga aagaaggctt
    33181 cccgttgcct tctttatttt tataaaaaaa taagctgttt ctaaaatgat ttcacatttt
    33241 tgaaacagcc aagttattct ataatgacaa ctataatttg aggatgtgag ggagagttgt
    33301 cattataaat ttagggtaat tatattatgt tttaagctag ttctttctat atttatataa
    33361 tacattttat atatgaattt tatatgagat tggtataaat gatatatgaa attacatatg
    33421 tttatattat attaaatttt attattacat tttccacaac aatactcata tgtctccaca
    33481 ttatttggaa tatttacaaa tacaaattct ttacagttct cacaatactt taatatagat
    33541 atagttccac aagtgtaaca ttcaaatatt acacaatcct cattttcctc tactatttca
    33601 aactttttac tatgttcacc acactctggg cacttgtgat tatttacatt cataaatact
    33661 cctccttatt agtatagtta catgtcaaaa acttaattaa tgtattctat attacttatt
    33721 atatattcta taataaatca tattctaata taataaaaca aattaaataa aaaaaaacca
    33781 taaattttaa atttatggat taattcttgt aatgataact ctatttgagg atgtgaggga
    33841 gggttatcat tacaataggg tattatatta tggtttttaa gctagtcttt ctacacctca
    33901 tatgatacat atgctatatg aattttatat gatttttaca taaatatttt atgaaattta
    33961 aattttttag tcattttgaa atttgtaaaa agttctctaa aaatccacag catcataaac
    34021 agccaaaatt taagtagaac atcataaaaa aatttgtcca ataattttaa caagtaatct
    34081 cacctcatta taattattga cacaaactta aataaacaat ctataaatct tctataaatc
    34141 ttaaactttc cccctattga tacacaattt ctagtttgtc aattttattt tgcatttcta
    34201 agaaaatttc ttcaacagaa ttgttaaata tattgcctaa atagattctt ttatcttttt
    34261 gaaatgaata agattttaca ctaccatcag aagaaatata taaatgactc cactctgctc
    34321 catattttga aaacctctcc tttgtatctt taaacctcat atcttctatt tttattgctt
    34381 tttctctgtc tcctcttact acagtgtgac ccctttttat taaactggtt atttttatat
    34441 cttcttttaa tttaatacgt gtatcatcat tttttgaata tactttccat atcactttat
    34501 gacgataatt atctaattcc tttattaagc ttctatcaat agtaaaacta tctttagtta
    34561 ccacaattaa atcaaaatgg tctaacatat attgaaatat ttctctcata cttttatttt
    34621 ttaaaatatc ctcaccaata acctctataa atctacagcc tttctcatac atgcagttca
    34681 ataaacttgt gatttcagaa aaataatcat ttgtattatt atctccatct atttctatag
    34741 taaagtacaa aggagtaatg tatgatttat ttcctgttat ctcaaataca cttgatagtg
    34801 tctgtatatc atcttcaaac gttaagtata cattctcata aaacataaaa aattccataa
    34861 ctttttcttt catattatgg atatttctat atctattgga taatatattt attatatcgt
    34921 cagtagtatt tttcccatca cattcagcca gtataacaat ttgcttccaa cttaagctat
    34981 ccacatttcc gatacttttt ttatatccat cttcttcatc ataaatagga ccaattataa
    35041 tgttttcttt tggccacaga tgaaccctag ttccatcttt taatttcgga tatttatttt
    35101 ttacagactc ataaaatcta cttgtatcac ccatgattct ttactacccc ccatagttta
    35161 aatattctat gctaaaaata gcactttatt tttattaatt caacaattat tcaaaattgg
    35221 tcattatata tgtttaaatg gatttaacat caaccttatt tatatgttat tgatgtttat
    35281 atatgttata taaaatctta tttctcatac ttcaatgtaa tcttaactat ctctggttta
    35341 caattaagcc taattaaaac ttttgagcct cctaagcctt tatttaaaat catagtgcta
    35401 ttatcttttt catattttcc ccagtcatac ttaggaaaga actctctatt tggagataat
    35461 acacctccaa caaaaggtat tcttattata cctccatgaa catgtcctgc taaaaccaaa
    35521 tctgctcccc attttgcata tttttcaaag aaaaatggtg tatgtgctaa aagaatgtta
    35581 tattcatttt cattaacttt acctagattg tcttctacaa aattgtcact tagttctaat
    35641 tttttgctat tagcaaataa atatggatag cattccaatg gtataattaa accatataaa
    35701 ttaagatggc tattacctct atatattctt acagattcat tttccatatt tattattttc
    35761 tttgaatata gttccttaaa ataatcctta tatagatgtt tatacttttt tattaaagat
    35821 ttttgttcat gatttccaat tatatgatat acttcatatt tttcaaccaa attatctatt
    35881 aaatctaatg caacttgaaa atttttattt tctccatcta ctaaatctcc tgttataaat
    35941 ataacatctg ggtttaatga ttcaatttta tctatcaata ccttattatt tttatcaaac
    36001 accttattat gtaaatcact cacttgaact attttaaagt tatcaaattc ctttggaatt
    36061 tttttgttga aaatagtata ttctgtcgtt tctatatgct gagtttcata cttatacaga
    36121 atatacaata atattaatac taatactgtc attgaaacct ttataatcat attctcttcc
    36181 tctttattct attttgttaa tattattata atgcttttat gtttttttta aaacaataac
    36241 atcatcacat acatataaat cttttcgaca ttataaaaat aagttatcct tttctaaaat
    36301 tttcatttat tttagaaaag gataactata aaattatcta taaacctcta tattgtgaaa
    36361 tatagttttt cctttattta aatttttata tatatgctct aaagttccat caaacctaat
    36421 agaatgtcca gactctaaaa tataagtttt cccatccaca ttaaggtcaa gtttaccttc
    36481 tattacgagt acatactctt ctaaaacatg actgtgtgta gatgaaacat gagtacagtt
    36541 ttcctcaagc tcaattataa aaagttcaaa ccttctttga gggctaaaag ggtatattgg
    36601 atataacttc atgttccctt tttgctcaac aataggctct acttcttctt tctttataac
    36661 ctcataggta tttgtatttt catttaatag tgtagtaaaa tttattttaa atccagatga
    36721 aattttccat aaagtagata ctgtcggact tgattcacct ctctcaatct gtccaagcat
    36781 tgctttactg acacctgtca tctttgaagc tttgtctaaa cttatgccca tttcttgtct
    36841 caataacctt aaattttctc ccacattagg aaatttatta tccatgttaa aatcctcctg
    36901 atagctattt gtattctatg atttttactc ctgtaagttc tactatctct tttaatttta
    36961 acatatgatt gttacaaatt tcatcaaatt catatatata atcttcaagc cctaatgcct
    37021 tatatttatc gattccaaat ttatgatatg gaagtaactc catcttgcct cctggaacat
    37081 tttgtttgac aaataatgca gtatttctta tattttcttc actatcattt actccataaa
    37141 taattggcac tcttataacc atagatttat tcaactttga aagcctacat atattatcta
    37201 atataatttt attacttact cctgtatatt ctttatgaat attatcatcc atagatttta
    37261 tatctacaaa tatgtggtct attttctcaa atacatcatt taccttattc caatcaaaat
    37321 atccacaagt ttcaattgca atatttatgc ctttatcata aaaaatatca actaattctc
    37381 ttaaaaaatc aatctgaagt gtaggttcac ctccagaaaa agttatccct ccattagatt
    37441 caaagaaaaa tataaaatct ttttcaactt cctctactat ttcttcaaca ctcatttctt
    37501 cggtcattat acaaatggca tctttaagac ataccttgat acactctcca cataaatcac
    37561 atttattatt tatttgcgct ctatcaaata gtgaacttat attttgagga caaacatcta
    37621 tacatctatt acaggataca catttatctt tcataacacc taattttact atgttactcc
    37681 aggaatctgg attagaacac cacttacatt ttaatttaca tccttctaaa aatatagtag
    37741 tccttattcc atctccatca tttactgaaa aatgttgtac ttttacaact ctaccttttt
    37801 tactcataat ctacctcata attaaaaatt gctatgtgaa tttctagcta ttatagcatc
    37861 ttgcatttct cttgataaat taacaaattg agtactatat ccagcaactc tcactaataa
    37921 atctttatat tcttctggat tttcttgagc ttttaataga gtatcatttg atatcgtatt
    37981 aaattgtaca tggaatcctc ctaaagcaaa atatgattta atcatagatg ttagatttct
    38041 aagtcctctt tcagtttcca ccaaatcttg attcagtctt aaatttagaa gtgttccacc
    38101 agtatgaaca tcatgattta tttttgatgc agatttcatt gctgctagtg gacttgttgt
    38161 atcagtacca acaaatggag aaactccctc tgtcaaaggt tttgtcgctt ttcttccaca
    38221 aggagttgcg cctacgattt ttccagttgg tacatagttt gatataccca taaatgcact
    38281 attaaattta gagccaaaaa tatccttgta ttttctagtt tcagtataat aaaaatcaga
    38341 aacttcaatt gctaatgaat ctacataatc attatcattt ccatacttag gaacatccaa
    38401 cgcaagtttt cttaattctt cataaccttc ccaatcatta tttagtgctt ttgttaattc
    38461 ttctaacgta gtcaccttat cctcaaatac taattttttt atagctgcca atgaatttga
    38521 aactacacct aaccctatac cagttaatac aggacctata ttatattttg ctcctccttt
    38581 acttaaatca actcccttat ccaaacatcc atctacacaa gtagataaaa atggtcttgg
    38641 taccatttct ttatgaattt gttgagcaac agtagttcct ataacagaac tttttacaag
    38701 atatgctaat tgctttaaaa actcatcctt aagttcttca aaagaagtaa attctgtgat
    38761 attttttgta tctaacccca ttttttctcc agttaatcta ctcttacctt cattcatagc
    38821 atattctaaa gctgctgcaa aattgatatt tacggctgaa gtccactgtc ctgtttttct
    38881 aaaatgagga actacacaac cacagttact ccaatctcta gcatcttctg catcatatcc
    38941 atcttgtaat aacatttgtg cccctgcact atcgctgtgt atagcaggga aaccagttcc
    39001 ttgttttacc aatttagcaa ctgccattac gaaattgtct ggtgctcctt gactcactct
    39061 aacacttaat cctggttggt gagtttttac actttctgta gcctttaaac acatatatga
    39121 gatatcattc gttccatcag taccatttct ctttttacca ccaacagtaa gattttggaa
    39181 ttgattatat cctgcaaagt aatttgctgt atttgctgat atagtccata cccattctga
    39241 taacttaatc caaagtgctt ctatcaattc ttgagcttct gattcagtta ttttaccttc
    39301 tctagcatca ttttcataat atggatacat atattgatca aatctaccta aatttaatgc
    39361 caatggattt tctgatagaa taccacctaa ttgaacaaac catacaaatt gtattgcttc
    39421 ataaaaatta gttggagggt ttgctggtac attcatacat atctctgaaa tttttataag
    39481 ttctaacttt ctcttttcat ctgtttcttg cttactcatc tcttttgcaa gatttgaata
    39541 tctttgtgcc aatgtcataa taccctgtgc aactatagaa acagatttgt aaaaatctat
    39601 cttttcaata ttttctgaaa ctgatatatc taattcttta atttttttat ctgcatcttc
    39661 cttaatccct ttatatcctt ttacaaacaa tatatcttga taatctggtg ttacttcacc
    39721 aactgcctgt ctccacttag aatcattatc tattattcct gtatctacta atatcttagc
    39781 tgtatcttct gggattcttg ctaagaaaac ttcttccaaa gattttccct tccaatatgg
    39841 aaatatttct tttcttataa attctatttg ttcttttgat attacatatg ggtcttgagt
    39901 tcttttatca aaagtatcca tttctttgtc tacccattgc caagaaaact ctggtgtcaa
    39961 tatccctgtt cttctaaatt ttcctgctgt acctactatt aattcatttt caaagatttt
    40021 tataggaagt tctttacatg tctccaaaaa agctttagct cttcttatac agatagcttc
    40081 tccctcagtt tttttatgtg attctgtaaa aattctagcc ctttcacaac atatcatagg
    40141 tttagaatta atatattctt gtctaaatgc ttctattcta ttagtttgac tcatttaaaa
    40201 tccccctttg gataatatat actacatttt tgtatgttat aacatacttt aattatatta
    40261 tactgtcctt tagcataaaa tggaagcaaa ataacttatt ttttttcatc taccttgaac
    40321 acctatatat taatactttt tttgttaaat ttataaaatc taaaaaaatt ttctatgata
    40381 tattttctat attgttatag ttatgtctaa tatactaatg atttgaaata tcaaatttgt
    40441 atactagact tagttttaaa accttagttg attgtttaac tttgttaact tcaacatgta
    40501 tatccttcat ataaggaagc tctacaacac ctactccctt attaaagtct tcttcaacca
    40561 tagaaaaaat ttctttctca gttaagttcg tatcagtcaa tgatttaaat aaattgtata
    40621 taaatttaac atatttatca ctctcagata acttatcatc ttctggatac tctttagcac
    40681 agagaatatc tattgttact tttccttctt tatcgtcttc agacatagaa tagaataaac
    40741 ttcgaatatt tttttcaggt ggtttttcag caccatttat agatactaca aagctaccat
    40801 ctgattttga gttagtaata ctataaccac tgtctattat agctttcgaa aatctttgag
    40861 ataaatcttt ttttacttct tctctacctt tcgcatcatt aatcttttgt gagtaattat
    40921 taaatagctc aatagattca tcttttttat cttcaacttg ttctttcaat gaatctttac
    40981 taatttgttc tgagctactt aagttcttat cagatacagg agacatacac cctaccatca
    41041 atgctaagct aagatttaaa gccacaatta ttgccactct atttatattc ctttttactt
    41101 tcatacacaa tcctcccatc acactaaccg ctctaaagta ttattcattg cactagaata
    41161 tatttatatt ttctttatat taaagattta ttattcaata catatcacct tcctatccaa
    41221 ttcttataac ttactattag taaaattata agctccttaa tacagaaata aggatgttat
    41281 cttttgttat taaaagataa catccttatt tttttgtatc aatacatatg tcccaactgg
    41341 taacttaatc gccccaactg tcagtatctt ggtccaatgg tatatatata tttagtataa
    41401 atttatcgat actattttca aataataatt ctccattata tttttgtaaa gaagatttaa
    41461 tactctgaat acctattcca tgtacgaact tatccatttt gtttgttaac aatatatttt
    41521 tattaaaagt aagctgattt agtttactat tttcacattt aagtacaaaa aatgaccttg
    41581 ttatagttcc tttaattcta atatatttat taatattttc atccactatt ttatcacatg
    41641 cttctattgc attatccaaa atattagaaa atatgcttga cacatctata ggttttacaa
    41701 aatttacttt tgaaaaattt atagagcaag taaatttaat tccttttttt aaacatactt
    41761 cactcttttc gttgattata atgtctaata tcatatttcc tgtattatag atatatttaa
    41821 atgtttttat ttcatctttt aaattgttaa catattcatt aatttctttg ctattgtttc
    41881 taaggttgtc tatacaataa atgtgattat ttatatcgtg atatagtttc ttaactcgca
    41941 tatgagattc ttgaatactt aaataatgcg catattgcat atctaactta ttttttatta
    42001 attcagcttg agattttaat ctattctctt ttattatttc tttaaacaat agtataagaa
    42061 atatactact agctcctacc aattttggat taacaaaatt atcaaaatag tatccttctg
    42121 aatgtaatga cccaaaccgt tctaattttt ttaatacata atttacaagc atattcattg
    42181 tattaactaa taaggcaaat aataaatata cataatttct cttatcttgg ctaattaatt
    42241 ttatagactt tctaaaaaag ctaaatataa gatatataag aataattatt aaattactat
    42301 atatctgaat ttttatagat tctaagtaag gtactaataa agattcttta gatgaaataa
    42361 cattacctgt aattgcaaaa aatagtattc tatatataac agcatccaaa catttataat
    42421 atacactaaa tagataaaag aaaatggaat acttaatata acccatctta taaaaataat
    42481 tatagtatcc tccccaagca ataaatgcta atatataaaa taaatagttt atattactaa
    42541 agtaatttcc tccattaaat aatctactta tctgaattat aatataaata agaaacaaga
    42601 caatatagaa tactattttt tcattttttt tactatcctt tttataaatt aaaattccgt
    42661 tcattataga aaactgtata aatatagata aaactactaa tagctcagtc ataaagttta
    42721 gcatattgta tcaccaagaa attttaagaa tttctcctta acttctttat atctatatct
    42781 actaataggt acctcttctc cactttccag tattgcagta ttaggcttta tattttcaac
    42841 atatcttaaa tttactataa aacttttatg gcatcttata aatttatatg gatttaattc
    42901 tttttctatt ttgcctaaac tatattttat atcaaaattc ttgtttattg tgtgtattag
    42961 catatctttt ttttgaactt ctatatattt tatttcattt aaataaatct tatatgtatt
    43021 agacttattt tttattgtaa tatgactttc cttatttatt tcaatatctt tgatacaagt
    43081 tagcacatgt ttttttagtt cttccaactc tattggcttt agtaaatacc tataagctct
    43141 tacctcataa ccttcttgca cataatctat taatgaagtt acaaatatta tttccatttc
    43201 attgtctact tctctaatct ttcttgcagt atccattcca tttaattttt ccatttttat
    43261 atcaagaagt aatatatcta tatcttttaa attactttcc agtaactctt ctccagatat
    43321 atacttttgt atctcatatt ctatacttat atcttttaat atattctcaa ggtaatcttc
    43381 tattaaaagt tgtgtttctt tttcatcttc acaaactgct atcttaatca taaaatgcct
    43441 ccttacataa tttctagtta atacaattaa aatatttata atatatttaa agataatttt
    43501 gtatatctga tactttagga taacattttt aaattatttt tatcaatact caatcccata
    43561 caactattat ttgatatttc aatttaaaga cagaaattac tataattaaa aacagtactt
    43621 actgacaaaa gaagatgtct taaaatatta aaaaaataat tttaggacac cttcttttat
    43681 ttatttaaac tttttaagcc ttaaataata cattaatctc ctatataatt gtactgcata
    43741 taaaatcaac aatataatct gtagtacaat agcagttaca gatgctccat aaccactata
    43801 taaatttctt atttcaatat tcaataaata tataaatatt actactaaca taaagaatat
    43861 aaacatattc ataaataaaa ttgactttac tttatttgtt ttatcagaat cactatataa
    43921 tttaggaggt atgtctttgt attctttcga ccatatatac catgcagatg tacccataaa
    43981 actaccaata ctcctaaata caagattcca gccatttgat ttatgttcat caaaataatt
    44041 attctctaca tcatagtagt aatcaacagt gtattttatt tttcttggac ttcccttatg
    44101 aaaataaaat attcctccaa atttatttat cttatataaa ttatatcctt tagcttcaat
    44161 atcttctaac catttctcta tcttatcagg tgaactatac cagtcaaatt tgaattttat
    44221 tttcttttcc atagcactca ttctccaaaa tgatattttt aaatagctta aaaatctatt
    44281 taaataatta tatcattttg atactaattt taggttacaa tcatatattt aaaagttatt
    44341 taaatagtaa taaactaata gccataacac ccattcctgc aatcaatcca taaatagata
    44401 aatgatgttc cccgtactct ctagctgatg gcaataattc atctagtgat atgaatacca
    44461 taattcctcc tacaattgca aatacaattc caagagttat atcatttaaa aaatttctaa
    44521 gaagtacata tccaataatc gctcctaaag gctcagacat accagataaa aaggaataat
    44581 aaaaagcttt ctttttatct cctgtggcat aataaactgg tacagataca gatatacctt
    44641 ctggtatatt gtgtattgca attgcaattg ctatcggaat agctattgta acatcatcaa
    44701 gagctgatat aaatgtagct aatccttcag gaaaattgtg aattgctata gccattgctg
    44761 taaatatacc tgttcttaaa agagacttat tttttttgat attcttttct tctatattct
    44821 ccattttctt tatttcatgt ggattttctt cttgtggaat tagcttatca ataacagcaa
    44881 tgatagccat acctataaaa aaagctatta cagttgacca acttcctaat ttctctccca
    44941 tagattttgt caaagcatct tgtgctttag gaaaaatttc tatcatagaa acataaatca
    45001 taacaccagc agaaaatcct aagcttatag aaagaaactt agtatttgtt tttttagcaa
    45061 aaaatgctat acaacttcct ataccagtag ataaaccagc caataatgtt atcaaaaatg
    45121 caaatattac attattcaaa taaatcacct tctttttaat tgatattgat tatcattttc
    45181 tttatgctat catatatatt ttatatagtc aatatttata aaaaatatac acaataaaaa
    45241 agcagagata taaacttagt ctctgctttt tctttatagt atattaaaat tatttattta
    45301 agaaatccac tgcatattgc acatataaag cagttccaat ttctagagca tcttcatcta
    45361 tattaaagca tccatgatga tgaggataac aagcaccttt tgactcattt ctagctccta
    45421 caaatgctag tgcaccaggt gctatattca taaactcagc taaatcttct gctcctgtta
    45481 ctttttcaaa tagtgttata cagtcttctc caaatagctt aattgctgca tctgtagcta
    45541 tctttgaaca ttctttgtca tttataactg ctggtgttaa gtacccatac tctaattcag
    45601 catctgctct atatgcttct gctgtagatt ttgctatacg ttctaaaatt ccaggaattt
    45661 gctttctaag ctctggatta aatagcctta ttgtaccttc taagatagct tcactcgcaa
    45721 ttacattaaa tctagttcca gaatttaaaa ctccaacact cacaactaat ggttccagag
    45781 gacttacttc tctactaacc atagattgta gattcataac tattgctgaa cttgcaagca
    45841 ctgcatcaac accttgatga ggaagtgaac catgaccacc tctacctttt acagtaattt
    45901 taaagaaatc agcagaagcc attcttggtc cttcttctac cgatatggtc ccagattcta
    45961 catcagtcca aagatgtatt ccaaacacac tatctacacc ttccatagca ccatcttgaa
    46021 tcatagctct tgcaccttta ccaacttctt ctcctggctg aaaaaataat tttacagttc
    46081 cattgatact atccttaata tcatttaaca cttttgctgc ccctagaagc attgatgtgt
    46141 gaccatcatg accacaagca tgcataagtc cttcattttt agatttgtat tctacatcag
    46201 tgcattcaac aacttgtaga gcatccatat cacccctaag tgctacagtc tttcctggat
    46261 tatctccttt tatagttgca attacacctg tacctccagc tgatacatat ggtatcccta
    46321 ttttatcaag ttcttcttta actctctttg atgttctaac ttcttccata cttttctcag
    46381 gattttcatg aaattccctt ctaagcttaa ttacatagtc tttataactc tttgttattt
    46441 ctttaatatc cattctatac ctccaagtag tacacattct tcttatttaa aatacttttt
    46501 atcaattaaa tactatatta gatttacaaa gatacctgct attactacag aagctattgt
    46561 aactgtagta aatcctccaa caagcatctt tggaagcatt ttatccatta aaaattcttt
    46621 ttcttctggt gtttcagcca aagcctttac tgcctcttca gttaatatat agtttggtgg
    46681 aaaaccataa agtgatgtaa gcgaaactga aaaagccatt tctttacttt cacctaaaag
    46741 cttacctact ataatagaga aaattcccat acctattaca ccaaatatta tgattcctac
    46801 aagaggaact gccatttcaa caagcattgc tggtgtagct tttgctaacc ctgcaaatat
    46861 aaaagccatc aatgatgtca ttaaccatcc aaatgaacca gatttattaa gtggttttct
    46921 atcaactatt ccaagttctg atgctattac accaaatatt aaacatataa catatcttga
    46981 tacaacttca tttgtaagat ttgtaaattg tactgctaac catgctacaa atcctaatct
    47041 aagtattatc atatatgtcg tatcatattt ttctggtaag tctggtatta tcttaaatct
    47101 gcttttttgt tcaggtaaat ccttaacttt ttcttcatct gtatttacag ttacttttcc
    47161 acttctaaag tctgataaaa gtctttttcc ttctttttta agcattaatg ctgtaatagg
    47221 ataacccgca aatccttgtg caacatacat caaaattgca agtactgata aacttgcaag
    47281 accttttgct gtagcagcat ctgccatcat tatagatgca actagccctc cagtaagagg
    47341 aggtgtagct attactgctg tatcccatcc aaatactact gtccctaatg ccaaagcacc
    47401 tacacatatt cctactattc ctgccaaggc tattactatt gttttccatt gacctgctaa
    47461 ttccttaata ctcatcatag ttcccatatg agttattaac aaaagcatac ctaattgagc
    47521 taatggtgtt cctaaagcgg ctaagtccac aatattttct gggaaaaaag tccaaaatcc
    47581 tactataaat ataattgctg atacaaaaac cgacggaaca aaagctttag tttttgctga
    47641 aactatatct cctatcgtca gtgatactaa aactagacaa aaagctgtta ttgcctccat
    47701 acaaaatccc ccctcataat aaatatattt ttataaaatg tatgtatagt tatccattat
    47761 tttactactt ttgaaaaaca tttcaagtca aaattcgatt tttttcataa aattcgataa
    47821 attccataaa ttaaagttag ttttctaaat tattcttttt gtaaaaacgt tatcccgacc
    47881 attggctatt taactctatt cttacaaaga gaattaaatg taatttgcaa aaaataaaac
    47941 ataggataca ttttattcat aaaacatctt ataaatataa aatgaattgt ctttatgaat
    48001 cttaatatat cctatgtttg taagtttaat gtgtatgtta gctatggtgt aaaatttgta
    48061 tactagttat tgtgtaacct actatatact ccatttttgt ttactagctc ttcatgagta
    48121 cctctttctt caattccttt atccgtcaat actatgattt catcagcatt ttttatagta
    48181 gtcaatctat gtgcaacaac taaatttgtt ctatctttac ttaattcctc taaagactct
    48241 tgaatttctc tttctgttac attgtctaaa gcagatgtag cctcatccaa tattattatt
    48301 ggaggattct ttaaaaagat tcttgaaatt gaaattcttt gtttttgccc acctgataat
    48361 tttacgcctc tttctcctat ataagtatca tatccatctg gcaaagtctc tacaaagtca
    48421 tgtattctag cttttttagc agcagctatc atttcttcat ctgtagcatt agggttccca
    48481 caaagaatat tatctcttat agttcctgta aataaaaata catcttgttg aactatacct
    48541 atattttttc tcaatgattt taaactaaca tcttttatac tctttccatc aatttttata
    48601 tcacccttat caaattcata aaatcttggc aatagattac ataatgtagt ctttcctcct
    48661 ccagaaggtc caactaatgc tatagtctta ccagctttta tagataaact taaattatcc
    48721 aaaacttgag tcttatcttc atatgtgaaa gatatatttt ctatctcaat gtcacctttt
    48781 acattttcaa gttcaatagg atttttagct tctttttgat ggtcttgttc cattatctct
    48841 atgaatcttt taaaacctgt cataccattt tgatattgct ctgtaaagtt tattaatttt
    48901 ttcataggtt gtataaacat ttttacatat agtatataag cagcaaaatc acccaaagta
    48961 atctttccta tataagtaaa ataaccacca gctattacaa ctacccactc taagatatcc
    49021 atgtataaat tcataccaga gtaatattct gccattacct tataagaatc ttgtcttgct
    49081 cttctaaata atttattgct tcttacaaac ttatttaact cttctttctc agtacaaaaa
    49141 gacttagtta ccttcatacc tgctatacta ttttctaaag tagcatttac atcacctata
    49201 gtaactctac tcttcataaa agcaatattc atcttatctt ttttagctat tgcaaaccaa
    49261 attataaacg gtaatatagc aaatattata agcgtaagag gaatatttat atctataagt
    49321 ataaagaatg aaccaaccaa cattactata gaaatgaata aatcttcagg cccatgatgt
    49381 gcaagttctg ttatatccat taaatcattt actattctag acatagttac tccagatttg
    49441 ttattatcaa aatacgtatt tggaagtctt tgtaagtgtg taaatacctc atttctcata
    49501 tcagcttgca tatctacacc aataacatgt ccccaatact gcataaaata gttaagccct
    49561 gcttttgcta taaatataag tataagtgct actgcaaaaa ctactaacat tcttaagttt
    49621 ttatttggaa caacgtcatc cattatattt ctagttatca taggataaac taaatcacat
    49681 aaggaaaata gaaaagctgc tataaggtct agtgtaaaaa ttttcttata tggcttataa
    49741 tatttgacaa attgttttat cataagtatt aaatccctcc tttattagta cttctaaaaa
    49801 atacccataa attagtataa cataacaata actttaaatc ttttataaac ttacaaagta
    49861 ataacaatct ttttcataaa attgaagatt tatatcatat agttgtatac ttgaaacaac
    49921 ataaaaagag ctatatctct atatttaaaa ttttataaag atacaactct atatttaata
    49981 ctaaattttt tttataaata caaaactact tcaatgcatt taatatcttt ttatctaatg
    50041 ctgttgtatt tatacctgtt atatatcctg ttgcctttat tgtaggtata tcataagttg
    50101 ttggtagtat agtagtagat acgattaaat cagcaccttc ttgttttgtt gaacattcag
    50161 caattttaca ttgctcaact tctgcttgta tattattttc tttaaccaat ctttctactc
    50221 tgtcacaaac tattgttgat gttgcgattc ctgctccaca ggctactaat atttttttca
    50281 tatattgtcc tcctacaatt tctttaatat tttataactc aaaataaaat tgataaattt
    50341 cttatatctt tatttatata aataattata tcattttata taagaagaac atcttaaaat
    50401 tattctttta atatgttttt tatttcaaca ttgcttatac ttaaaatttc attttttata
    50461 caagactcta tgtactttga tatattttta taatcattta aattaaactg ttcaatattc
    50521 aatttatatt ctatatcact gacaatacct atcaaatcag acttattaga taaaatatcc
    50581 tcatacttac cttttctagt tacttccaat cttttaaatc tataattttt atatccttct
    50641 atgataatca agtccatatc ttcatataat tgaatcattt tatataatgg attcttctct
    50701 tttagttctt caatatatgc tactcgttta tctgatatta tacagacttt tctcgcccca
    50761 gattttctat gtttataagt atcttttcct tcctcatcca tatcaaaatc atgacaggta
    50821 tgtttaataa ctccaacacg ataccctttt ttatttaatt cttttattat gccttcaatc
    50881 aatgtagttt tacctactcc tgaatatgag acaactgaca atatattata cataaaattg
    50941 tgtcctttca taaaactatc taataacaac atccactaat tgattttctt ttattgtctc
    51001 attcttttta atcttaatca tacaattact tcttagagtt gcagataatc tagcttttgc
    51061 atctttctta tcatttagct ctacatacaa tttaccttca cttgtatata gttttccact
    51121 tacaaatcta tctttatttg aaagtttatt aaattctcct ttaaatatag cttttatatg
    51181 atgtgttccc ttcttatatg ttaaatactc aataacagga actatcacat tatcaaatac
    51241 cactattgct gcatgtggat taccagaaag accaaataat agcttttctc ccaatgtacc
    51301 tacataacaa gctccaccag gtttaatgtt tatcttccaa aataattctg ttgcccctat
    51361 ttttttgaat acatctggaa ttaaatcttt atctccaaca gaaacaccac ctgtggtaat
    51421 taaaaaatct acactctgta acatattctt tacctttttt tctaactcca atatatcatc
    51481 agaacaattg catattgtat ctgcttgtat tccaagtttt aataatcttg aatataaggt
    51541 atatctatta ctatcgtata ttttaccatt ctctaaagtc tcatttatat cttgtagttc
    51601 tgtacctgta ttcaaaattc caacttttaa atcatcataa acagttactt tggatattcc
    51661 aagacttgct aatactccaa taccatcata tcctatattc tgatttttat taataatcaa
    51721 ttgccccttt tttatatcct cacccttaaa tatgtaatta tcgtattcct ttaattttat
    51781 atttaaagtt atcatatcac ttataactgt tacatcttct ttttttatta cacaatttgc
    51841 accctgtggt atctttccac cagtcattat cttcactgct tcatttttat ttaataccct
    51901 attaaataca tctcctgcat acaaagtccc tataaccttt aaatttctat tttcagaatt
    51961 tgcattatat gcataaccat catagggtga cctatcaaat ggaggactat caattgttga
    52021 atatatatct tctgcaacta ccttatttac tgcatctata agatttacta tcttgaccct
    52081 gtttattggt aaaacatttt tttcaataat ttttatggca tcttctaatt ctatcatttt
    52141 aaatactccc caatatattt tttatttaag atatttctat gtaatatatt atagattata
    52201 aaagttggaa atacttttat cttatattta attataacta atagttatct ataagtgcag
    52261 ttcaaatttt ataaatctaa taaaaaaact gttcccagct agaaatttcc ttctatcttg
    52321 aaacagcatc atatgcttta gaatatttta ttctaataaa gttcctttta ttatttttac
    52381 aattttatca ctttcaatag tttttataga ttttattttg ttatccacaa tacttattgt
    52441 aagtacctct ttttttaata ttttgttatc ttctttcaat ctataactct taactacttt
    52501 actgccttca tgattatatg ctgttaaatc ataattcgca tctcctacaa tttcatttat
    52561 tctggaatct tcaacaccaa catactctcc caggtcaata tgtctaaaat tttctagtga
    52621 tacagccgat ttttcatggt cacttttttc aatcactata ctagattcag catcaatgac
    52681 tgatatacct tgtgatgaga aagaattggt ttcaacattg acaactttat tttcgttaat
    52741 atcaacatat aaagcacttc catctagttc atcatttcca gtatattttg gataaactaa
    52801 tgtcatataa cttgatgtat ttatatcttg cattgtattt gattctttta aaactttagg
    52861 ttttatataa taaaatgtat tataaggtat acccatattc tttataatgt acttataatc
    52921 tttattcata atttcattaa catcattttg aacttttgtc atagtttttt gctgtgttgc
    52981 tttacttgct acttgatttt tctctttagt attttcttta tgacatccaa caagtataat
    53041 acctaaaata gttacacaaa ctaatgacct ttttaacttc ataaatcaat cctcctttct
    53101 taaattttat agtatttatc tatatctcaa tttacagtta tctctttttt caataattat
    53161 atttattata ttataaaata attatataat atttatttga atttaataag attaagaagc
    53221 taattgtaat ttttctgtaa taaaaaacac tttaaagttg aattttgaaa attcaattta
    53281 cgatattttt attgtaagta ccttacaaca ttataccaaa ttattcctaa acaatcaaaa
    53341 actccatgaa tatttaaagg ttttctattt taaaaatcac ctttgactat taatttatca
    53401 aatatgttat aatagtttta gataatttaa ggaggattaa ataatggatt ttaaacaact
    53461 tgaagtattt gttgctgttg caaaacatca gagtttttca aaggctgcaa gagaactttt
    53521 tttaactcaa cctactgtaa gcgcacacat acagaattta gagagagagc tagaaactgt
    53581 tcttataaat agaagcaaca aagttattac actcacaaaa tctggtgaaa ttctttatga
    53641 acatgctata tatatattga ataattgcaa acgtgcaatt tatgatataa aagaatattc
    53701 aggaaaaata gaaggtatta tagatatagc atgtagttcc atacctgaaa cttatatttt
    53761 acctgatttt atgaagagtt tttctatgag ttatccagat gtaaaatttt ccataagcca
    53821 ttatgattct caatatgcta tatcagaaat tttaaatgaa cgaataagct ttggattagt
    53881 tggttctaaa atcaataatc ctcaaataga gtacctagac ttattagatg atgaacttgt
    53941 tcttatcact ccttcagatt ttaaaattga taataaaaat aattgtatcg atataggaga
    54001 attggcttat ctaaatttta ttatgagaaa agaagggtca ggtacaagaa acttaatact
    54061 aaacacactt agtaaaaata actttccagt aagtaaatta aatgtaatcg ctcatgtaga
    54121 atcaaatgag gctattaagg aaatggttag attaggtctt ggagtgtcct ttatatcgta
    54181 catttcagct atcgattatc tcaatgcagg taaaataaaa tgttataaaa taaaggatgt
    54241 tgattttact agaaagtttt tcttcattta ctctaaaaag aaaacttttt ccccattaga
    54301 agataaattt ctaaacagat tatgtgagta tttcgaaatt ataatataat aactttaata
    54361 aagtagtgct taattttgag tttataacta attaataagc taattataac tatcacataa
    54421 atgcttataa attattatga accatctttc ttttttattt aatttagtca aaaaatccct
    54481 cgttcaaatg aacttgggat ttttttattt atactactcc aatagcagcc aatactatac
    54541 aaataatcat aactgaaatt aatatcgtag ttgtctttac cttttttcct aataaccaat
    54601 acataatacc gaatatcata gctggaagca tacatggcat tatttggtct aagtaagttt
    54661 gaataggctc tgcccattct ccactaccta cattaacact aagttttaac attacattag
    54721 atgcagtcat acctccaatt accataagcc ctaacattga agctgcttcc ataacttttg
    54781 aaactatact tgaaccactc acatctttaa agaattgagt tccatactta aatcctttat
    54841 ctagacatat atagcgtata ataaaatgag gaacatttat tattaaaagg aataaaatcg
    54901 gtcccattat gttaccctga gaagctaaag ctataccaac cccagctgct ataagcttta
    54961 atgttcccca gaaaaatgaa tctcctatcc cagaaactgg acccattaaa cttgatttaa
    55021 cagcatttat actattagca tcaaattctt tattctttgc attttcttct tccatagctc
    55081 cagatatacc taccaaaaga gtacttatat gaggagtaac tgacataaat tctaaatgtc
    55141 tttttaaagc cttagatttc ttttctttat catctttata taatctacgg attattggtg
    55201 ccatcatgta tgcatatgct atattttgca tacgttcgta gttccaagat gaatctaatg
    55261 tcaatgacct tctaaaacat ttccaaaagt ctttcctcgt tattttacta tctggtgaaa
    55321 ttgtctctaa tttcttagaa ctcgttgtca tctactatta cctccttctc tcctacaaag
    55381 ttaacatata ttaaagctat tgctaatcca aacaatgcaa ctcctgttac tgttatgttt
    55441 aggtaagcaa ccaatataaa tcctaataat aaaaatgcta ctgttttctt attaagcatc
    55501 attctagcta acatggcaaa acctattgct ggaatcacgc ctgctgcaac atccattcca
    55561 tgagttacat atgatggtat aaatgaaagt attttttcta ttactggaac acctaatgaa
    55621 aatcctatac ctactattat tgctaatatt aatttatttc ctataccaaa taataattgc
    55681 acacgttcta cgccttcaat attcccctct tctgcataac ggtcagcaat atgagctccc
    55741 catgtaagta ctactgtcat taagaagtta tctataagca atactagagt tgctatagga
    55801 actgcaagta caactgctgc tgatacatct tgacccgtta atattacaaa cgctgtggca
    55861 ataatagaac cagacacaaa atcaggagga ttacttgccc caacaggaaa tgcacccata
    55921 aatactaatt ctaatgttga acctattact atacctgtat gaagattacc tagtgctaaa
    55981 ccaacaagtg gtgcaacaac tataggtcta ttatgcattg tagaacctac aaaactatgc
    56041 atatatgcaa agaatgcaat tagagttatc aaaataactt gtgtaaattc cattttcatc
    56101 cccctaaaat tatattaatt tcattacatt tgtagcatca tcatcaggaa ctaattgtac
    56161 aaatacattt acgccttctg aatctaactc tttaatcatc tttatatcat cttttgatac
    56221 atgcactgct ttagatatat tttcacgatt atcatctgat tttgttccac caaggtttat
    56281 agatttaata gcttttacct ctttagcaag tctgtaaaca tcttctacag attcacaaag
    56341 tatcaataag ttgtatttat cagtaacacc tgagttaagt gcttttatag aatctgctat
    56401 gcttttcatt actaacttaa cattagatgg tttagccatt ttaagtcctg ccattcttaa
    56461 ttcatctttt aataagtcat cacttgctat caatatacag tctgtactta aaaactttgt
    56521 ccatgtaaat gctacttgtc catgtataag tctgtggtct actcttacta atttaatcat
    56581 tataattcct ccctaatttt tttgatttca tccatgaatg tttttacgtg ttctacacta
    56641 acatccccaa aatctttgta attatcttta aagtaacttc ctattattgc catatctcct
    56701 aatttaagct gtttttttgc attttctaat gtaactccag cagctataac caatgggaag
    56761 tctcctaaat ttttacgaaa cagttctatt ttttccattg atgtttcttg acctgtagca
    56821 ttttctgtaa ctgctatagc atcacatctt gtcattccta tttttaaatc ttcttcaaca
    56881 tcattttctg ataatactgg ctgatattta aaacgtacgc ctccaattaa atacgctgga
    56941 cattttgaac gttgtaattt aaaaaactct tctaaagtag cttcatctct aggtttaaca
    57001 tgacctacta cagaatcaac ctgtatataa ctagcattat attttgttgc cagttcaaat
    57061 cccatagtat caacatttaa acagtttact ccatatggta ttgataaatt agcttttgat
    57121 acatattcaa gtattctttc taaatcataa taattaccat agtaattttc taacattatt
    57181 ccatctacac cattttcttc aaagattgta atctcttttt tagctctttc aaagatatct
    57241 tctggagtat ctccttttaa gtgtatcatt gctataattg gttttttact tttaaatact
    57301 gataatattc cctttttttt cataaactaa aatacctctt cttcttcttt aatttgtaat
    57361 gtcacgcttt gtattcctct tctagattct tccactaatt ggtctatatc ttcaactgtt
    57421 aattcatcag aaaaccttgt aagaagttct aataccatat tcaaattcat tccagaaaat
    57481 aacctaacat tatgtagatt agttaatctt aacattgctg tacatacact tgctccatat
    57541 aagtcagata aaattatata ttcacattct ttatcttctg atattgtttt ttctaattct
    57601 aaagcaaaat ctgaagcact ttgtccaggg tacaaaccat acgaactgac acaatctgct
    57661 aaatctccta ctatcatttt tactgcattt aaaagccctt ttgaaaagtc tccatgagat
    57721 gctaatatta tctttttcat actcatcctc ctactaaatt atagattata tttctgctga
    57781 tactgcatat ttgtacttat cacttcttat aaattgctca ttgtaatata agacatcatt
    57841 attttctgta tacaccgatt gtcttacttt caatagtggc atattacttg cacatttaag
    57901 atggtcagaa tattctttgt ttgaatgttt tacttcaaat atattttcta ctcgtttagg
    57961 taatgtgctt atattattag atataacctc cattaaagat ttatcttcta attcttcttt
    58021 taataaaaac atgtaatcta ttctaaaata gtcaacttca aaaataacag gtacttcatc
    58081 tattaatcta agtcttttaa tacatattac cttctcttct agttcaatac ttaaagcttc
    58141 agctacttgc ttatctgctt tttttattaa aacagagatt attttggtac ttggttttgc
    58201 attaatacga tgacaagaat tagtaaaact atttcctgca catgttgctt ctattctctc
    58261 tggtattgtt acataagtcc ctttaccacg ttttttacat aatatatctt cttttacaag
    58321 ttcattaagt gcatttctca cagttatacg acttatatta aattctttac ataagtctcc
    58381 ttcagatgga agtttatctc ctggtttata tatgccagat gctatttgtt tttttatgtt
    58441 gtcatatagt tgttgatata aaggaacaaa ggaatttact tctaaatcca tttctttttt
    58501 ttgtctcttt tcatccattt tgctcacctc cctatgtaac atttttataa cattatataa
    58561 caagttcttt attacatatt ataacaagat gaaatcgttg tcaatttttt tttaaataat
    58621 ttttaatttg acagtatttt cttttatttt ctctagtgtg acaaattgct atttttacat
    58681 aaaaataaga ctgttgtaca acagattaaa ttgtttatac aacagccttt attcacttat
    58741 tacatttata tttttactta taaatttata ttaaaaaatg ccttatatcc cttatacatt
    58801 tcatatatgt ctactttaga gacaatctca tgtggtgcat gcatatttaa aactccaaca
    58861 ccacagtcta taacctctgc accttgattt gccaatatat gggctatagt tccgcctcct
    58921 ccttggtcta cttttccaag ttcagcagtt tgccatatta catttccttt attaaaaatt
    58981 ctctttactt ctgacataaa ttcagcatta gcatcattac atccactctt tcctcttgaa
    59041 cctgtatact tagttaaaac tactccattt cccatatatg ccgaatttct cttttcatat
    59101 gcttctccaa aatttgggtc atatccagca gaaacatcag ctgataatac cttactattt
    59161 gctaaagctc ttcttacttt aatatcacaa taatctcctt ctaaagcaat cagttcagcc
    59221 acagtatttt caaaaaactt tgagtgcata cctgtattcc ctttagaacc aatttcttct
    59281 ttatctacac ataatgcaac tgaagtaaat tctggttgtt cagtttctag tatagctttt
    59341 acagcagcat atgaacatac cctatcatca tgcccatatc ccaacaccat agacctatca
    59401 agccctaaat ctctagcctt accagctgga actacttcaa tttcagcact taataaatct
    59461 tcttcaacta tattgtattt ctcatttaaa atctttaata tatttgctgt tataggttct
    59521 ttttcttcat cttctaaagg catatttcca attaatatat ttaaagcttc tccactaatt
    59581 ccttctgata gtttcttttg catttggtct ccagccaaat gaatcaataa atcagttaca
    59641 caaaatactg ggtcactatc ttcttcccca atagatatgt ccacttttgt tccatcattt
    59701 aatataacta taccatgtaa agcaagtgga atagccgtcc attggtattt ttttattcca
    59761 ccataataat gagtcttaaa aaaacctaga ttactctctt gatacagtgg atttggtttt
    59821 aaatctaatc ttggcgaatc tatatggctt cctataatct tcataccttt ttctatataa
    59881 tttttaccta ttacaaaaag tgctactgcc ttctctttat ttataacata aatcttatca
    59941 ccacaactta tgcttccttt ttccatagct tcatctattg aaatatagtt attttcttta
    60001 gccaatttta taatttcttt tactgaaagt ctttcagtct ttgacttact taaaaactcc
    60061 atataattgc tagaatattg cataatttgt tcaatagtat tttcttttct ttcaaaatcc
    60121 caagcattct taaattcgta ctttaagttc atattttcct cctactaagt tctaattttt
    60181 attgtttttc atgtattgct catattcttc tggtgaaact actttgacac tatctagtct
    60241 tgatctaaag tgggctctaa agttttctaa atattctttc cttaaaattt ctctttcttt
    60301 gatttcatca gcagaaagga ttccctcttt atttttcttt gctaattcat taatcctatc
    60361 taatttcttc ttgtcaaaca ttttttcctc ctaaaaaacc tctatttatt taataaaata
    60421 atttattgct ttttatatct aacttgtttt tatattcaca aaaaaactca tctatattat
    60481 cacttattat agatattccc ttttctagtt cttccatgct tgggcgagct atatttatcc
    60541 taaagaatct atcatccact agattgtcaa aaaaataagt ccctggaagc attgacactc
    60601 ctttgtttaa cataaaattt gtaaatgctt gagatgtata tcctcttggt aattctaaaa
    60661 aaaagtttat tcctccacaa gattttctaa cttttaattt accactcaat ttgtcattaa
    60721 tcaagttttt tgccaattta tacttatcag tatatatctt ttctatatga ctcaaataat
    60781 tcttccaatc aaaattattc atatagtaat acattgattt ttgaattaaa cctggtgttg
    60841 atatatctga tgaatattta gcccataaaa ctttttttaa caactcattt ggcatttcta
    60901 caattcctat cctaagacct ggcataagta tttttgaaaa gctttttata tatactactc
    60961 tatttttatc atcataactt ctaattgttc tattgtcctt tgattcaaaa acaaaatcac
    61021 ttataaaatc atcttctatt atataaaaat catgttcttc tgccaattct atcaatttct
    61081 tttttttata tgttgagtaa gatattccag ttggattttg aaaattaggc ataatgtata
    61141 taagtcttgg ttttattttt tccaacttta attttaaaat acctatatca attccatcat
    61201 ctaacatagg tatagatatt atttttgcac ctctactttt aaacacttca attgctccat
    61261 tgtaagaagg ttcttccata aaaacaacat ctgaataact tataagtcct ttacaaacta
    61321 tatctatacc ttgttgagca cctgatatta tttgaatatt ttccttagta gtttttatat
    61381 ttccatcact tagatactct accatttttt ctcttaattc ttcagagcca agaccttcat
    61441 catattcaaa tattgaacta ccatctttag ataatgccat atttacagca tttttaaatg
    61501 aatcgatagg aaacatatca ttagatggat tcccattgtc aaaatgaatt aaatcttctc
    61561 tttgcacaat aatcttattt ttcctgttat ctataccgct tttataagaa tttaaacttg
    61621 atacaaaagt accactccct ataattttat atacatatcc ttcattttct aaaagctcgt
    61681 ataccttaac tatagtagtg ttatttactt gtaaaaaatt tgatagtttt cttattggag
    61741 gcaatttttc atgttctact aactctttat ttattatcaa ttcttttata tgattaaata
    61801 tctgtatata tttagttgat tttttctcat ttaaatctat tctgtatttt tccaaattta
    61861 tcaccgctca ttgtatttat attttattga tgcttttatg tatatttgat attatttagt
    61921 atgtatatat aataccaaaa tttttataaa aaggggaaat atttatgttt tacgactatc
    61981 acatgcactc aagtttctct acagacggaa aatctacaat ggaagaaatg gtaaaaaaat
    62041 ctattgaact cggtttggag gaaatttgtt tcacagacca tgttgactat gatgtatatg
    62101 ctgatgattc tttttcaatt gtctacgaag attattttaa gagtttagag actcttcaaa
    62161 gcaaatataa agataaaata tcaataaaaa aaggaattga atttggagtt caaactcaat
    62221 taatggatac atacaaaaag gaagcacatc aatatccatt ggactttata atatgttcta
    62281 tccatgctat agatacaatg gacttatacc ttggtaatta ttttaaagat aaaactcaac
    62341 atgaagttta tgaaaattac tatctttatt tatataatat tgttaagaat tataaagatt
    62401 actctgtctt aggtcattta gatttgatta aaaggtatgc accttatgac actattttag
    62461 atgatagact attttctgat ataattgaag aaactcttaa acaagctatt tatgatggca
    62521 aaggtatcga gataaatacc tcttgttata gatataatct tccagaccta accccatcta
    62581 agtatatact tcaaatgtat aaagacttgg gaggggaaat cataaccaca ggttcagatt
    62641 cacatcatat aagtcaagtt gcatgtgagt ttgactacat atattcatta ctaaaaaata
    62701 tgggatttaa atatgtatct aaatttaata aattgaaacc agaatttata aaattataaa
    62761 tttttacacc tccttgtgaa tatatttaaa agataaggag gtgatttttt tgaaagtacc
    62821 ttatttagta ctatcaacag ttattttatt tatatccaat tttattgtaa gaatatttgg
    62881 ttttttatat aaaatattct tatcaagagc actaggtgaa acaggtttag gtatctatca
    62941 tatgattttt aactttttga tgatttgctt agctgttaca acaacaggta tacctactgc
    63001 acttagttgt ctagttgcaa agagaaaagc tttaaacgat agacacaata ctaatgcttt
    63061 atttatatca actttatata tatcattttt cgtagcacta attatatcta tagttgcatc
    63121 tttcaatagt tcttttttat cacttaaatt tttaaaagat gctaaattaa atctatttat
    63181 actagctgtt tgtcctgcta tagttataat aactctttca aacgtactaa gaggatatta
    63241 ctatggcata aagaatgtaa aaatccctgc tattggacaa atcattgaac aaattggtaa
    63301 aatattattt gtttttttac tagttatgta cataaataat aaatctatga attgttatat
    63361 agctctatta ggtatatcaa tcggtgagtt gagtaatatt atatttatgc ttatttgctt
    63421 atggcgagat tcatcatttg acaataggta tattataagc ataaaagatt tttataattc
    63481 ttctatggaa acattaaaaa tgtctattcc cataacttgt aatagaatgt caaacatact
    63541 tttgcaatct ataagttcta tgatgatacc atcaagatta gcattatcag gaatgactta
    63601 tcaacaatct ttaagtatgt atggtattgt cagtgggatg gtaatgcctt ttatattttt
    63661 accttttact gttggttcag cgcttattgt aaatttaata ccaactattt cacaagaaat
    63721 ggctctaaga aagcgtaaga gtgtaattaa aaaaatcaaa tactccatac ttcttacttt
    63781 atttgtaggc atactatcat caatattttt ttatttcttt ggaaaagatt tatgtatatt
    63841 agtatttaaa aatacacttg caggagaata tctaaaagct atgtttttag ttccactatt
    63901 tatgtcccta aatcaaactc tatctggcat tttacattca ataagaaaag aattagcttc
    63961 aagtataaat actattactg gcatgttaat tcaactaata gctttatatg tatttcttcc
    64021 aattccagga cttaatattt atgcatacat atacacaatg acgatagtat caatctttac
    64081 ttgtctatta catactatag tgctttttaa atcactaaaa tctatacgct agttataaaa
    64141 acgtgaaggc taccaatgta aattatacac tttggtagcc ttccttagat gatttttatt
    64201 tataatttaa actgtctata tatcttacct atctatctgt ggcaacttgg ctttccactt
    64261 ccatgattag gtccacattg cgaaaaatca catactttag agaaagtatc aacaagtctt
    64321 ggttggattg gatttggtga tgctaaaact actatttgtt cacttattaa tgtgctaact
    64381 gtttcttgta agcaaagtac taaatctata tcatttggta taattaattc atagaaaggt
    64441 tcacaacatt cgttgcattc taattttaat atagattttc ctatacaatc tacagttaag
    64501 ttagtttgat attcttgtct taaagtaaca gctcttccgt cagctggcaa gcatattggt
    64561 gctgaaagtg tgttaaaagt aaattctaca taatcaactc ttctcttaca tccacctcct
    64621 gctctaactg ctgggaaagc taatgcaacg aactctgtac atccacatct acctcttagc
    64681 tctagtacta aattacgaac tgctacagtt aatcctcttt gtttataagc tactgatttg
    64741 ccttttcctt gtctacagca ctcagcgttt ctttctccac aaactgcaaa ttcaaaagtt
    64801 gtttcacagt ttcttcctac tatatctcct aatgggtcta aatcgaaatc ttctaaagtt
    64861 gtcattcctg tgtctataac gattccatca ttacttaagc atattttttc tatagttaca
    64921 cttgcttgac ctgcacttgg aactgaacca aatacttcta ctacttctgt ttcaaaagtt
    64981 aatggctctc catttggtcc tgttgcatct gtaaatgttt ggaattgcat agtatcaaat
    65041 attctattag cctctattgc aaatggcact gcatttgata cagagaagtt tcttggagat
    65101 ataggattac agcattctgg tccacatttg ttttcaaaac attcatctga atccatttca
    65161 catggttcac aatcacattt acaattgtca tggcatccac aattgtcatc acatggatta
    65221 ggtttgcatg gattgcatgg gttaggttta catggattac atgggttgca tggattgcaa
    65281 tcatcatggc actcacactt attatcagta ttacatccat tatgatggtg tgatttttta
    65341 aaacctctca tgtcttctga atttatttca tctgtataca cttcttcttc attcattgta
    65401 gacattggct gattcatcat tcttctttta ttttttttat aatcttgcat ttttcattgc
    65461 cctccctgta agcgattctt attaagtctt tggctttttg aagcctaatt aatactatgc
    65521 ttttataatc ttaattgtta cttgcttttt gaatttatca ataattaggc gtatttagta
    65581 gaaaaatgca tattaataaa aaaagtacta ctatttgaaa tagtagcact ttttttgtcc
    65641 cgaaacaccg gtggctaagt aaattcacac cctaggcact cccaggtggg tgacttgcca
    65701 gtaaccttta acgtccaaac taactaggca tgttataatt taccagacac ggtctcccgt
    65761 atttcatttg taggttgaat ttaaaaaagc cttatcgtat gtttcaggat ttcatattat
    65821 tattatataa taaattttga attttttaaa gtggttttat atagtaattg agataatctt
    65881 attctatcat tactttttct tccttttttc tttatgtatt tagttaatta tcgttttttc
    65941 ttagctctag ttttagtatt tttttcatta taattgtatt cggattcttg ctcattaatg
    66001 ttgtatagtt cctcatcata tactatatct tcatcatttt ctatattttc atagcctgtg
    66061 ttcttatctt ctactaattc atcttcatta tcctccattt caccttcgtc tgttaactca
    66121 tcataatcat catccaattc tttgtcttca caatctaatt cttcgtcttc ataatttaac
    66181 tcatcattat tgtcttcatc atcattatta ctatcatcac ctcttaaaag acttcctaac
    66241 aatttgagat tattcatact accaagattg tttatatttg ccaaagagct caaactaaat
    66301 ccaccttcac caaagtcatc tggtaaaaat aattctgcaa ataaatctat cttctttttc
    66361 attcctatag ctttttcaac tgaatttttt cttttttctg ggaatacatc tataatttcc
    66421 tttatcatca tattcttttt atcatcacta tccatttttc tgaaaaaatc cgtttcatct
    66481 tctgtatcta ccatatctat attatttaat ttttttattc ctctaactaa atctaaaaca
    66541 ctttctattt ttattagtat ttttctttca tctcttgaca tgtatttttt agttcttgtt
    66601 attatttcca ttcccatttc taaatcttct tcacttaaat ccatgagcct taaacctctt
    66661 tttatatcat ttgcatcaaa ccttaaattg ccatgtgtag actgtgaaag tttatcattt
    66721 gcattttttt tgttttttct aactctatta tttccttttg atttattttt atcatcaaat
    66781 atagagatat taccattact caatgcaatc actccaatca aaaatagtaa gttattcaag
    66841 acaattcccc cttatattat ataattgccc tatattaaag tatgtttttc atattttaat
    66901 gttccatttt taaagctcag tgcatatata ttatctataa gaatttttat ttatattata
    66961 tatcactcac aattcaccac ttcaatatta tttctatata taaatagtat gaatataatt
    67021 caatatatat ttatagaaag gggttttttt atgaaaaaat tagatacaga gtcattagaa
    67081 aaaatggcta aacaaaaaaa tattgacaag gataaaatag aaaagatggc tgatagttac
    67141 aaaggaaaat cagaaaatga acttatggag gaacttataa aaataggtaa gaacctagat
    67201 ggcagagatg aagtagtatc aaaattcaaa gcatttttag atgaaaatca acgcaaaaaa
    67261 ctggataata taatggaaaa aatctcagat gcagaaaacc aaagagatac taaaccagct
    67321 aaaactaaga aggctaaacc tactaaaggt aagtcaaatt cacctgcacc aaaaaataca
    67381 ccaggtagtc aacaaaaaaa atctaaaagt ttatttaaga aaactaaaaa gtctaaccct
    67441 catgaataga taaaaataaa ctatttaagt aattagataa ttatttttag ttaatctatt
    67501 attaaatttg atattattga cataaaaaat caggtagtgc cttaggcact acctgattga
    67561 ttttaatagt tttatttttt tctttctgta acactttctc tatcaacaat tctataaggt
    67621 aattctattt tcttattctc aattaattcc ttatttatta actttataac cattcttatt
    67681 gctactgctc ccatatcgta tagaggttga tatactgtag taagtttagg tctatacatt
    67741 ctagctatct taacatcatt aaatcctgca acagatatgt cttctggaac cttatatcca
    67801 gcatcacata tagcatttat agcacctatg gcagcctcat cacctgttac aaaagccgca
    67861 tgaggtatta ttccatcatc taatagttct ttcatactat tgtatccact ctcataatca
    67921 gttccaccat acttaatcaa actcttgtct aattctatat tgttatttga aagtgctttc
    67981 tcataaccag caagtctttc catttctaaa acagtatcat ctttactcgt cattataaaa
    68041 gctatctttt catgaccttt ctttataaga tgctctgtca tatcaaaagt agcttcttcg
    68101 ttgctagtac ttactgtata tatatcataa tttctagcag ttttacttat atatgtcgct
    68161 ggtattccac aattttgtat atagttgata tgttcttgtt caactatcca tgaaatcata
    68221 actatacctt ccacttgttt tgctctcaat agatttatac tcttaagttc ttgttcctta
    68281 tcagagtatg tattcgctaa aagaatatca tagtcataca ttttagcaac ctcttctatc
    68341 ccatttaaca cctcattaac aaaagaatct gaaacttctg gaactattac ccctattaat
    68401 tgactcttct ttgttactaa gcttctagca agtggatttg gtatatatcc agtctctttt
    68461 ataacctcta aaactttttg tttgacttca tcagttacag gttttgaatc atttataact
    68521 ctagatacag tagatattga cactcctgct tgtttagcaa catcttttat cgttatattg
    68581 cctttcatct tcatcctcct tattttacta aatttatcta aaactgaacc aaatttttga
    68641 aggttcctat tttaactaca tttatataag tttttatttt accataaaag caagtacatt
    68701 taaatacatt atattttata ttatacccta tattcaacaa atttaataat attcattctt
    68761 aatttaaatc atattaaaaa ataaaataaa aaaatgctgg tataaaatac cagcattttt
    68821 ttattctaat gatttaagta ttagcagccg caacctactt caccatcaaa gaatccacca
    68881 catgaacaag ttattagcaa taatattaag aaaggtatcc atgacattat gtcaatgtct
    68941 ctccagcaat cttcaaaagc taagaatagg aagaatagta ctattatcca ccatcctcct
    69001 ccaccaaagc atccttcttc tccaaaaaat ctatctaaca taacaaagcc ctcctttttt
    69061 taataactgt gttcttattc taagcatcca ccacagcagc aaagtattaa taaaactatt
    69121 aggaatggta tccatgccat tatgtcaaat tctgcccagc tatctccaaa agctaagaat
    69181 aggaagaata gtactattat ccaccatcct cctccaccaa aacatccttc ttctccaaag
    69241 aatctatcta acattatacg ttacctcctg ttgatatttt gttgagcttt tcgctcctaa
    69301 catatactat gcatttggtt tccattttgc taatgtattt ttactatttt tttaaatttt
    69361 ttaatttgaa ctaaaaaagg taaaaccata acttgtataa gttactggtt ttacctattt
    69421 aataatcaat attttttagc aacaacaacc atcatcgaag ttaaataata acaatactat
    69481 taatataacc catatccata tattgtcatc gcataataag tcttctaaaa tgtctgtgca
    69541 taatagtaag aatactataa ctattattat caacatttta ttcccaaaaa atccttcgca
    69601 tcctccaaag aaacgttcca tattgtcttc taattttgtc ataactaatc ctccttgaat
    69661 cattaagaga ctaagttttg tctctttaat taaatatatg tagtaataga cagataagtt
    69721 acttctaaaa agtttttatt gattagttta tttatttctt tttcaccaag tttattgtat
    69781 ctatcaactt catccagata atcttttttc tcctctgata tagtatagat taaactttct
    69841 attgatttta ttttgtactc cttatatctt tctatcaaga aggatgtttt tgaattttct
    69901 attcctttct ttacatgcat taaccttgat atgtagtctt ctttttcatt agatacataa
    69961 ctctcctttt cagctacatc ttcaaggatt aaattggtct ccattacata atcctctata
    70021 cgttgcatat ctaaagttat tgaattataa taaaaattta ctgctataag taaaaacatt
    70081 cctactgaag ttagtaatag tcctacatat ttcatagcat ttcctcctaa gttatatttg
    70141 ttatcaataa agtttattca tcaaataata ctttattact acatttataa aattttatta
    70201 ttttttatag attccatatt gaaaaattct tgatattata ttaaactaac attaaaatta
    70261 attaaaactg ctttaggagg cttaaaatgt ttgagaaaat aaaagaaata atagcagaac
    70321 aattaggggt agacaattta gatgagataa ctatggaagc atctttaatg gacgacttag
    70381 aagctgattc attagatgct gttgaaatta taatggccct tgaagatgaa tttggaatag
    70441 aaataccaga cgaggaagct gagaacttta aatgtattgg tgatatatgt aaatatatcg
    70501 aagaaaacaa atagtatcgt tttgtctaca aatataaata gtctgtgtaa acttctacat
    70561 aataaattat attagagttc gcacagacta gattacattt atttacaata tataaaatac
    70621 acgctcttat atttaattta tatactaagt atataaatta cttttcttca taaaacgtaa
    70681 cattctttat tattatatct atagttccat cttgattttg ttttacagta tacttcattg
    70741 ggtcttcaaa atcagttaga ttccctttta tgtcaaaacc attatccgtt tttatacttc
    70801 tttttttaag ctttttctca acccattttt tatctatact aaaactttcg tcaagacctt
    70861 tttcttccat atgctcttta aagctatcct ttaactcatc atctttaatt gaattatcaa
    70921 caaagtcatt tatatcaact tcatgttttt ccttcagtgt ataatttaat atacttctta
    70981 catcttcagc ttgctttata tcattactaa gagcattggt tatccaattt tcagctgtat
    71041 ttttaaatac cttggtcttg tacttatcat catctatttt tttagcattt aaaaattctg
    71101 ttacaaactt agattctgac ccttcttttt cagcatcctt atctaaaagc cttagatgaa
    71161 actcatcatt tataccactt agactaatca aagctccttg cttttgtctt aaagtctctg
    71221 ggataccaat ttcatttgaa accatttgaa tattaaattt atcatccaca aattcaattg
    71281 aatgagtgta caacttttta taatctaatt tcagtattgc tacactcttt tcatctttaa
    71341 tagtgtataa acaaattgcc aaatcacaag attctagtgc agcattaatt ttcataacgt
    71401 caaacaagta agatgcaatt tctttagaat ttttcaaaaa agtactttca tcatatatta
    71461 tttgttcaca acagttttta attaaattgt cattataatc tttaaaaaca ccttttctta
    71521 aatcctcatc ttttgcaatt ctttttatag ctttctggaa aaaaccatcc acttcttgat
    71581 taactttgcc ttcaaagtca tttaatattg gaacatcact atttttgtcc aaaacatgta
    71641 ttataaattt atgtattatc atatatatca ccccattatc ttaatttcga tattaactct
    71701 ttcatatcct ctggtatatc tgctttaaat ttcaattttt cttttgttct tggttgttca
    71761 aactctagct tatatgcatg aagtgcttgc ctatttatta gctcttcatc tacataacca
    71821 tacaactcat cacctattat tccatgacct atatgagcca tatgtactct tatttgatgt
    71881 gttctaccag tttctaagct tacttctaat acagttgcat tttctagtct ttctattact
    71941 ttatagtgag taacagatga ctgtcctctt tcatctatta tcctctttat tgaatcttct
    72001 gtaggtctat aaattggctc atctatagtt ccctcatttt cttttataat tccatcaact
    72061 actgttatat actttttttc aactttattt tcactcattg ctgtagacaa agtatgatgt
    72121 gcataagcat tttttgctac tataactaac cctgaagtat tcatatctaa cctatttaca
    72181 aatcttattt ttacgttttc tttttgatta tctatatagt aactaatacc atttgctatt
    72241 gttttatcat agtggctttt tgttgggtga actaccataa acggaggttt attcaccata
    72301 attatatcaa aatcatcata aattatctgt aaatttaaat cttgaggttc aaaatttgct
    72361 ttttcctcgt ctatttttac ttctattaaa tcaccactat acacctttaa actcggtttt
    72421 ttatatactc catttactaa aacactcttt tccctcttca tttttgataa tgacctaact
    72481 gagaaattta gcttgtccaa taaaacttct tttaaagtca tttcttcttc atttgtatac
    72541 gatataagat tatatctttg attctctttt ttaaacaaac ttttacctac tttcttttat
    72601 atcttcctac tttttatatc ttcaaaatca tgatattata tattttacac tatttttgtt
    72661 taatagataa taattatttt taactataaa aacctgtaag aataacaaat agttattctt
    72721 acaggttaaa ttcataatta aatatttaat ttgtaaatat tataatctaa aactatctct
    72781 taactattaa agtagttccc attcctccac ctatacaaag tgtagcaaga ccagttttag
    72841 catctcttct cttcatttca tataaaagtg tagtaagtat tcttgctcct gagcatccta
    72901 ttggatgtcc tatagctatt gctccaccat taacattaac tttattcata tctatattta
    72961 agtctcttat tacagctaca gattgggcag caaatgcctc attagcttca actaaatcta
    73021 tatcttcaat agtcatatta gcagcttcta aagctttttt agttgctgga actggtccat
    73081 atcccattat tttagggtca acaccagctg ttccataaga aactatagtt gcaagaggct
    73141 ctattcctag ttcttcagct ttttctttag ccattactac tagcatagca gcaccatcat
    73201 ttattcctga tgcattacca gcagtaactg ttccatcttt tttaaatgca ggtcttaact
    73261 tagcaagttt ctccattgta gtgccaggct taatatattc atctttatct actacagtgt
    73321 caccttttct tccttttata acaacaggaa ctatttcttc atcaaatttt ccttcagctt
    73381 gagctttttc agctttattt tgacttgcaa gagctaattc atcttgttct tctctagtta
    73441 tattccattg ctctgctatg ttttcagcag taatacccat gtgatagtta ttaaatatgt
    73501 ctgataatcc atcttttatc attgaatcaa caaaagcagc atcacccatt cttgcaccat
    73561 atctcgcact tggtactaaa taaggagaca tactcatgtt ttcagctcca ccaactaaca
    73621 ttatatcagc atcacctaat gctataagtt gagatgccat tgaaacagat cttaatccag
    73681 aaccacaaac tatatttata gtcatagctg gtttttctac tggtattcct gctcctaatg
    73741 ctatttgtct tgctatattt tgtccaagac ctgctgtaag tactccccct aaaagagatt
    73801 catctatcat atctggagtt atgttagctc tttttatagc ttctttagct gctgttaccc
    73861 ctaactctac cgctgaaact gatttaaatg ctcctccaaa acttcctact gctgttctag
    73921 ctgcactggc aattactact tctctcatat tcattacccc cattaaatta gatttttcat
    73981 atatccgaaa ttgtttttta aacaatttta tttaaacttt taattcccaa attcttattt
    74041 attataatca tagaatccta tcttagtttt tcttcctaat tgattagctc taaccatttt
    74101 agctaaaagt ggatgaggtc tatatttagt atctccaaat tcagtatata aaacgttcat
    74161 tatagctaaa acaacatcta atccgattaa atcacctaat gctagtggtc ccattggatg
    74221 gtttgctcct aatttcatag cttcatctat ttcttcttta cttgcaacac catctgcata
    74281 tataccaaca gcttcattta tcataggtat aagtattcta tttactacaa atccaggaga
    74341 ttcagataca tctactggta ctttattgat actcttagat aattcaaata ctgtatcaaa
    74401 agtaactttt gatgttaact gaccacttat aacttcaact aatttcatca taggaactgg
    74461 attaaagaaa tgcattccta taactttatc tggtctctta gtagaagaag ctatttctgt
    74521 tatagataat gatgaagtat ttgttgccaa gatagtatct tctttacata attcatctag
    74581 taacttgaaa acatctttct ttatattcat gtcttctaca gatgcttcta ttattaaatc
    74641 catatctttt aaatcttcat agttagtagt tgaactaaca tgacttaata tttctgcttt
    74701 tgtagcttca tccatttttc ccttagtaac taacttagtt aaatttttat ctaataaagc
    74761 taaacattta tctatagcac cttgagttct actctttaaa catacatcat gtccacaact
    74821 tgcaaaagtt tgtacaatac cacttcccat agttccacta cctattacag ctaatttcat
    74881 agttataacc tcctgttatt ttagtattta tattctgtta actagattaa aatactattt
    74941 aatctagtta acagcaaata tatttttaat tagattttta cccttttata aagttagctt
    75001 ctctcttttc aacgaaagct gacattcctt ctttttggtc tttagttgaa aaacaaagac
    75061 caaataaatt agattctata tctattccag tatttatatc agtttgagca ccaagttgta
    75121 ttgcttcttt agagtatcta actgcaagct gagcattttt agctattatc ttagctaatt
    75181 tctcaacttc ttctattaaa atgtctggct caacgactct atttactagc cctatttttt
    75241 cagcttcatc agcttttata acttgacctg taaagattaa ttcttttgct tttgccattc
    75301 caaccaatct tgtaagtctt tgagttcctc catatcctgg agttattcca agagttactt
    75361 ctggctgacc aaatttagct ttagcagatg caattcttat atcacatgcc attgcaagtt
    75421 cacatcctcc acctaaagca aatccgttta cagcagctat cactactttt ttactatttt
    75481 ctatttctcc aaaagctttt gctcctaaga tactaaaatc tttagcagct acagcatcta
    75541 aatctttcat gtatgcaata tctgctccag ctacaaatgc ctttccttcc cctgtcaata
    75601 ttacaacatc aatagtttca tcattattaa tatctacaaa tacttcataa agttcttcta
    75661 aagtctttga atttattgca ttaagggctt taggtctatt cattttcact gtacatatat
    75721 ttccatctac ttcaacagct acattctcat aaactttaac atcacttgta ctcataatac
    75781 cctccccaag aaattagtta gctaaaactt cacctttttc ttttgcaaca cttaactgac
    75841 tgataagttc tggaagcact ttatgaacat ctccaactat accaacatca gcatatttaa
    75901 atattggagc ttctggattt ttatttatag caactataaa ctcagcatct tccataccag
    75961 ctatatgttg tattgctcca gatataccac atgctatata aaggtctggt cttacagttt
    76021 taccagtttg accaacttgt cttgctttat ctaaccaacc tgcatctata gtggcacgag
    76081 aaccagaaac ttctccacct ataatttcag ctaattcata aagtatgtct aagttttctt
    76141 ttccacccat tccacgtcca gcagaaacta atatcttagc atcttctatt ttaacttgtt
    76201 ttttagcttc ttttattact tgtacaactt gaactaattt atcagcatca ttaaattcta
    76261 ccttgaaacg gttaattaca gcttctttag tttcatcagg ttcatttttc ttcataaccc
    76321 ctggtctaac tgtagacatt tgaggtctga aatctttaca aactattgtt gccattatat
    76381 ttccaccaaa ggcaggtctt gtcattaata ataattttgt atcttcagct actgcaagac
    76441 ctgtacagtc agcagtaaga cctgtatgta ttctagcaga aactctaggt gctaaatctc
    76501 tacctattga agttgcacca aataatacaa ctatagggtc agctgctttt attgcttcat
    76561 aagctgcttt tgtatatggt tcagttgtat acactgctaa agcttcatca tctactacta
    76621 ttacctcatc tgcaccatag tgtgctaatg tatctattaa accttctacc ttactaccta
    76681 aaagtaatgc agaaactttt gtatcataat cttttgctat ttctgtagcc tttcctagta
    76741 attctaaaga aacagtttga attacatttt ctctttgttc tattactact aaaacgttac
    76801 ccattcttta tcccccctta aattatatga tatacttctc ttttaattta tctatgataa
    76861 ttccagctga tgttttcgca tcttcattgt atattgtacc agctggttta actgattttg
    76921 taaatgattt aaatacacta gttggagaac ctttaagacc taaattagaa gggtcaactt
    76981 ctatatcttt tacagtccat gtttctacta catcattttc gaaagcatca tatattcttc
    77041 caactttcat gtatcttggt gtgttcatat ctttaagagt tgttataagg catggcattt
    77101 taacttttaa gtcatggcaa caatcttcaa attgtctttt tactaataca tattcacctt
    77161 cagtttttat ttcttcagca tatgttattg atggaagatt taaatgttca gctatttgag
    77221 gtccaacttg tgcagtatct ccatctatcg cctgtcttcc agctattata atatcaaaat
    77281 ctatattttt taatgctcct gctaatgctg atgaagttgc ccaagtatca gcacctgcaa
    77341 atgctctatc tgttaataat atacctctat ctgcacccat tgctaaagct tcttttaaag
    77401 ccatatctgc ttgaggaggt cccattgtta taacagttac atgagcaccc atttcttctt
    77461 ttaattttat agcttcttct aaacctgctt tatcatcagg gtttattata cttggtactc
    77521 catctctaat taaagtacct gtattaggat ctagtttaac ttctgttgta tctggaactt
    77581 gttttataca aacgactata ttcataaatc cttcctccat atctatttta atatcgacct
    77641 attgtgtata ttttaatatt tatctatttt aataattttc ctgaaataac cattctttga
    77701 acttcactag ttccttcata tatttcagtt atcttagcat ctctcatcat tctttctact
    77761 ggatagtcac gagtgtatcc atatcctcca tgaagttgta cagcttttgt agtaacttcc
    77821 atagctgttt cagctgcaaa taattttgcc attgctgctt ctactccata aggttttcct
    77881 aagtctttat ttatagctgc ttgatataca aggtgtctag ccgcttgtac cttaacttcc
    77941 atatcagcta attggaattg tgtattttgg aattttgata atggtctacc aaattgtact
    78001 ctttctttta catatttaac agtttcatca agagcacctt gtgctaaacc taaagcttgt
    78061 gcagctatac caattctacc accatcaaga gtagacattg ctatcttaaa tccttgacct
    78121 tcttttccaa gtaaattttc tttaggtatt ctgcaatcct caaatattaa ttcactcgta
    78181 gctgaacctc ttatacccat tttcttttct ttaactccaa agctaaaccc aggagttcct
    78241 ttttcaacta taaatgctga tattcctttg ttccccttag atttatcagt cattgccatt
    78301 actacatata tgtcaccagc tattgcattt gttataaata tttttgagcc attaagtatg
    78361 tattcatccc cgtctaaaac agcagttgtt tgttggccag acgcatctgt accagcatta
    78421 ggctcagtaa gaccaaatgc tcctaatttt tctccacttg ctaatggtct taagaatttt
    78481 tgtttttgtt cttcattacc atattgatat ataggccatg agccaagaga tgtatgagct
    78541 gataatataa ctcctgtagt accacaaact ctagacaatt cttcaactgc cattatatat
    78601 cctacagtgt ctccaccttc tccaccatat tcttttggat atggtatacc catcattcct
    78661 gcttttgcca ttttttccac tgtttcataa ggaaatcttt cttcttcatc aagttctgtt
    78721 gctaaaggtt taacttcatt ttcagcgaag cttacatata gctctttaag catctgatat
    78781 tttttagaat ttaaatccat aatagcctcc tcagtatttt ataaatattt tattttcatt
    78841 tattaattaa aatctcggca acatctataa taaggtattt gaatatacgt cgagaaattt
    78901 ataaccttaa tcaaactacg ctctatattt atatgtgatt atactaacca attatgacat
    78961 taatttaatc ataaattgtt aatttaaaat caactatact taaaaaaaat ctcgcttttt
    79021 ggaattttgt gaaatttcaa gttagaataa gatttctttc acactggtta gagataaaat
    79081 aatttgttta cgatatttat attaaggtat attaaacatt aaatattgtc tgtacatctg
    79141 ttttttagcc atgtacagaa gatttttatc gtataaaaat tattttaata gagctaaata
    79201 atcatatttt tatctgacta ttttttattt ttaaatatct tacatctata atagtatcac
    79261 tcaaaggata atttttcaat aatttctaat tattttttta acaatattta ttacattttt
    79321 tccacttttt tgtcattttt ttattgtttc gaaaaaaaat cagaattttt taactttatt
    79381 ttggattatt agtttaaatt agattttcct cttagattta taaatattta cacaaaaaaa
    79441 ctacctctgt atagattgta tacagaggta gttttttact aattcctact atagtttact
    79501 atattttttt accaattctt acaaacatct acccattttt cacacttaga aaacttctcc
    79561 atttcttcac atgtaagttc tattgcagag ttactactgc cacatgcagg aaatactgta
    79621 tcaaatcttt tcattgaatc atctaaatag acttttatag aattttttaa accaaatgga
    79681 cagactccac caatagcatg tccagtaaac tccaagactt cttctggtgt caacatttta
    79741 gccttacaat taaattcagc cttatacttc ttattatcaa tcttagcatc accagctgtc
    79801 acaattaaaa ttgcatcatc tcctatttta aatgagagtg tcttagctat tcttgctgga
    79861 ataaccccag ctgcttcagc tgcaagttcc acagtagcac tagattgttc aaattctaat
    79921 atactgtctt ccttgccaaa ttgtttaaaa tactctctta cattacttat tgacattaaa
    79981 tacccctcct agtctgaact tttataaatt tcatattatt cttaattata tctataattg
    80041 atttattttt caaattattc cataatatta gcattccatg tatcattatt ttttccaact
    80101 actagataaa gtccttttgc cattggaata ttgacaatat attctccttt atactctaca
    80161 ctcataccag attcattaaa gtacctataa tcaaaattta gtccttttgg aattacaaaa
    80221 tcaacataac cttgattatt tggttttatt ccatatttca gagttctttc agttatagaa
    80281 tttttttcat taaataaatc tacatttaca tatgcagaac acttattcat tcccttttta
    80341 ttttccacac aattttcaaa cttcatttta gcactgttta atttataact atatttacta
    80401 ttattgctta ttttaaatct tacaatagta ccaacatctc tacttctcct gctatcaaaa
    80461 tcaaaaacaa tttcctctaa atgatttatt tcataatctt tgacacttac catatctcta
    80521 tatactaatt tatttatagc tatgaatagt gtaaaaatcc ccaaaataaa aaatgaaact
    80581 tttataaact tcttcatttt tagtctcctt atattttgtg ctattgtgct ataatattaa
    80641 tcaaaatttt aataattaaa cttattcttt tttgaaatct taatagaaaa atacattgat
    80701 attataaaaa ttattattgt taaaaatgtc atactatata aatttatata cttagtatta
    80761 gctatatcag ttacataatt tgaaagatat actcctccaa ctttactata attaaaatgc
    80821 tttacttgat atacaatcat actaatcatc ttcaaaagac ttccatatac aaagtaaatt
    80881 aaaatactta ctataaaacc acaaactttc atttttccat atccaaactt aaaaattaaa
    80941 ggcagtaaaa tcgtcaaaat tgctaaaaat atatttacag aaaaaaatac atcatttaaa
    81001 accatatttc taaagaacac tagtgatgtc aatttactta gtaaggacac aattattgtt
    81061 acagaaatta ttaatcctac acctaataaa tattttgagt aaactatatc ttctatacta
    81121 actggcatac tccttataaa aatccttgaa tcattttcat aatcacactc aaatgtattt
    81181 ataaatatca aataactaac aacaatactt actgtataat atgaaattga attaaggaaa
    81241 aaataaaaca caaaaaatag caatatgtac tttaaattac ggataccatc tgaactaatc
    81301 cctattatta aatcttttct tattaaattg aacatcatct cacctcattt acataataat
    81361 acattagttc ttctatagta ggatttttga ccttatgctt atcaatttct atcaataggc
    81421 tttttacttt caagctatct ccaactttaa caagtgcctc attataatat ttatactctt
    81481 tcacaccaac tatgctttct ttaattgctt ctaactctgc tttatttgcc ctaattatct
    81541 tatattcttg ttctattaat tctttcttaa cagtaaaaat ctgatttcct ctatttataa
    81601 aaacaagata atcagcaatt ttaattaaat tatcagtatt atgagtagaa attattacac
    81661 tactacttcc atttttcaca aaatcttgta agtactgcat tatttcagtt cttacaattg
    81721 ggtctatccc agcagttggt tcatccaaaa tcaataactt agcattgtga ctaagtgcac
    81781 ttgataacat aagcttttca gattgacctt tagataaatt ttcaatatgc atattttctt
    81841 ctatatcaaa cttattaagg tattcatcaa acctttctct atcaaaattc ttataaaata
    81901 aagaaactat ttttctaaat tccttaacct ttaaatgcgg ataaaattgt aaagaatcat
    81961 atacaaaacc aatattgttt ttaaagctta ttggagaatt ttcaatagtt tcattacaaa
    82021 gttcaatttc accttcatct gcatcaataa tccccattat aatttttatt aaagtcgttt
    82081 ttcccgaacc atttggtcct ataatcccaa caatatcccc ttcctttaac tctaagttta
    82141 tattatttaa cttgaatttt cctaattttt ttgtgatatt ccttatgcta aacacatctt
    82201 cacctcttat gcattttttt atacccacct atacatattt taacacattc actacaaagg
    82261 tcatttataa atttataaat taagaattat aaaactatgt tataatacaa agtatgaaga
    82321 ggtgaatata tgaaaagaat tataactata aatacaaacc aattaaataa gtcccttgag
    82381 actaaagaac tcttaactag aaaacttatc aatgctggct ttgaagtgta ttcagatatt
    82441 tatccagata cagagcttat tatatcaatt ggtggtgatg gctctttttt aagaacagtt
    82501 agagatttcg attttcctga aattcccata atgggaataa atacaggtca tctggggttt
    82561 tttcctgata ttttaccaga taagattgat agctttattg aagcttatac aaaaaaagat
    82621 tatattattc aagaaatgtc acttcttaat gcggaagtat atacaactac tagtggttct
    82681 aatatgcttg ctgtaaatga ggttgttata agaggagaca agtctaggac tatacaccta
    82741 aatttaagcc ttgataataa acatatacaa aattttagtg gagatggtat gattatttct
    82801 acttccacag gttcaacagc ctataactat tctgctggag gtagtattgt tgatataaat
    82861 cttgaactta tgcagattac acctttacat ccgataaata ccaatgcata taggtgcttt
    82921 acatcaagta taatatgttc aaatgaatct gttataaaga ttgcaccaga atatagattt
    82981 gaagattctg tattgattgt cgttgatgga gttgagcaca gatttagaca aatcgaaaat
    83041 ataaaagtta gtatatctga tgcaaaaata aaattactta gaatgtcaaa ctatgagttt
    83101 tggcatagag tatctgaaaa atttttatag actgtactta ggaggatttt taatatgaaa
    83161 ataggaataa tgagtgatac tcatggtagc ttactttact ttgaaaaagc gttaaatgta
    83221 ttatctgatt gtgatgtttt attacatggt ggagatgtct tatatcatgg acctagaaat
    83281 gatattccag aaggatataa tcctaaaaaa tttattgaga cattaaataa attagaaaac
    83341 attgtgattg taaaagggaa ctgtgatgca gatgttgacc agatggttat agaacatcct
    83401 attcagagtc cttatgtcat gagccaattt ggtgaaatta gaattatatt gaatcatggt
    83461 tatatagaat cagaagaaga aattattgac aaagcaaaga aaatgggtgg tgacatatta
    83521 gttttaggac atactcatgt aaaaaagcta tacatggatg ataatttaat agtaataaat
    83581 ccaggaagta cttctatccc aaaagatgga agccattctg tcgcaattat agatatcata
    83641 aaaactgatg atgaggatga gcttgaactt gatataaact taattgacat caatactgga
    83701 aatataataa atatcaatga ttagtaaatt tttaataaaa ataaaataag ctttaattta
    83761 ttaaactcat aataaaaaca agtaaaccat tttatttcta tctcaattag aagtttaagt
    83821 acaatacctt aaacttctaa ttaaatagaa tctggtttac ttgttttatt atatgctata
    83881 ttgaaatata acttagttta cttatttcat tatatattat actgaaatat aacttagttt
    83941 acttatttta ttatatatta tattgaaaca tagcttagtt tacttatttt attatatata
    84001 ttgaagtgca acttggttta cttgctatta ttatatatta attttgattt acacaatatt
    84061 actgtaaggc taatgcttct gcatatggtt tactattttt acctccacca acttgtacaa
    84121 atttatcagc ctttactttt tttataaaac ttttctgtgc actacttaaa cttccacttg
    84181 caagtactac aggtgacttt gtttttgatg ctaatactcc agcagcaagc ccatcaacta
    84241 aatcagcttc tctaccactt ccatcttttg caaaatacat attattaacc ttacttgatt
    84301 ttccataaaa cttttctaaa actttcgcat tagtatcttt tctatctgtc cctgctagtc
    84361 tttctgagtt tactaactgt ccttcaagtt ttttacttat actgtactcc ccacctacta
    84421 tgtatgtctt agttgagttt tgtgtaataa aattctttgc ctcacttctt atgctatcct
    84481 tacctgtata tattatagct gtattatcac ttgcagctgg gcttgatatt gaagttgcat
    84541 ctgccagtcc attataccca ttaaccactg ccacttttga gacacctaaa tcaccagtat
    84601 catctagctg tctagtaaga agtaaagatg tttcttctct atctgctcca ccgattctac
    84661 tgactaatac ttccatatct tcaagagatt cttttacact tgatgaaata gctccttcac
    84721 ttccaattaa aataacttct gctggattaa gcctttttat ttcctctgtt acattatatg
    84781 gtatactatt tttatttgta agcagtattg gtgcatccaa tttacttgcc aaaggagttg
    84841 aagccaatgc atctggcaat gaatttgagt taactagaaa caccctttct gaaccattat
    84901 tccaaccact ttgagatatt tttatcgctg tatcaaatct atcttcacca ataagttttt
    84961 cctcctttgg agattttcta ccttgaagat aattgttcat atctacctgt gcattttgat
    85021 ttttactaat agtgtctgca tatacacaac ttggcaacat aatcccaagt gaaagtgcaa
    85081 atgctattat ttttttagat ttaattttca atacaattcc cccttttaaa tgttaatacc
    85141 tattgatatt catacttttt attgataccc taaactaaaa ataattatct tttataaaaa
    85201 taattacaaa aatagctata ccattttaag tacaacacaa gcttataatc agcttgactt
    85261 ataaagaatg atatagctat tataacttta aatgcttaat ttttaaatac tttttaacta
    85321 aactctttca gcatattttt cagctattct tacaattagt ttagtagctt tctccataga
    85381 actaactggt atacactcat ttttcccatg gaagtttaat ccaccagtaa atatatttgg
    85441 acaaggtaat ccattaaaag ataatcttgc tccatcagta ccacctctta caggaactaa
    85501 acgtggtttt atacctaatt cctccatagc ttctttagct atatcaacta taaacataac
    85561 tggctcaatc ttttctttca tattgtaata agagtctttt aaatccaatt ccactctatt
    85621 atcgtatttc ttatttattt tttctatatt ctctatcata atagattttc tattttcaaa
    85681 tttttcttta caatggtctc taataatata agccatactt acattctcga catttccacc
    85741 tatagaattt aaatgccaaa atccttcata tccttctgtt gtttctggtc tttcatctgc
    85801 tgggaacatt tctgatattt cagcagctat atgtatagca tttaccattt tatttttagc
    85861 tgaacctgga tgaacatttc taccatgtat agtaatggtt gcatttgcag cattaaaatt
    85921 ttcacattca agttctccaa ctattcctcc atctaaagta tatgcatatt ttgctccaaa
    85981 tttttcaaca tcaaataaat cagcacctct accaatttct tcatctggtg taaatcctat
    86041 ttttatatct ccatgcttaa tttctggatg atttattaga tactctattg cagtcactat
    86101 ctcagctata ccagctttat catcagaccc aagtagagtt gttccatcag tgactattaa
    86161 atcgtcacct ttaaattctt ccaactctgg agaatccttt acatacgtaa taacatttaa
    86221 cgcttcattt aaaactatgt cttttccatc ataattttct attattcttg gtttaatatc
    86281 ttttccacta acatcttcaa ttgtatctaa atgtgatatg aatcctatag tatccacacc
    86341 atcagtattt ccttttaaag tagccattat ataactattt tcatctaaac tcacatcatc
    86401 aacacccatt gtttttagct cttcaactat atacttagct aaaactcttt gaccttctga
    86461 agatggacag ttctcacact gagaattaga tgttgtatca aatgatacat attttaaaaa
    86521 tctttccact actttttgtt tcacgtatat accctctttc ttaataagat taatattttt
    86581 ttatttacct gtgaataaaa ttttattttt aaaaaatcta tcaattatta taattaaaaa
    86641 atatccacct tcttaagtat atcatgcata cataccaaac ccaaacaaaa aattactcca
    86701 attattcctt ttacttggat tccaataaaa atcgctgcta gggtagctaa tggatttagt
    86761 ccaacattta ctgatactag tttaggttca agtatttctc taatcaaaga caaaacaaaa
    86821 aatatcacag tcatacttat agctataaaa taatttttta taaggaaata ataaacaatt
    86881 actggaagat atattgttat tatacctagg aatggaatta aatccattaa agctcctaca
    86941 aaccctacta ttagaccgta tttcaatcca aataaagcaa aacttcccca gattgcaaaa
    87001 aatgttatag ccataagtat tgtataggcc cttatatatc caacaagtga aagccctgcc
    87061 tctttcttta cgttttttac cttctttcta acatcaacag taaacatatt ataaaaacta
    87121 ttttcaatct tatcaaggtc ttttgctata aagtacgtag caataaacaa ggtaattata
    87181 aatattataa tatatggaat agaagtagcc aatccaagta tactagtaag aagatttttt
    87241 gtaatttgca ttacttcacc actgaatttg tttactaatt gttctacatc aaaattggct
    87301 atttcttgaa atgatttcat aaagtcattt attttattta aaaaatacat aatagtatta
    87361 cttatatcct caggattagc agatatatta tttaaaaatc ccattaattc tttcattgaa
    87421 tttattacta ttattgtagt aactgtactg actatagcca ctgcaagaaa acttagaaca
    87481 agagtagaga tacctttatt tatacgaaac ttattttcta acttttgtga tataggatta
    87541 atcataagtg ctattatagc acctagaaaa aatggagcta tgtatggaaa cgctttgtaa
    87601 ataaaaataa ataacaatgt atatatacaa aaaaagatag tgttgttttt taatttgtat
    87661 aaaaaattat tttctagtat gctcatctta aaaatctcct ttcttctaat ttatattttt
    87721 attttgtcca tttcatacta gttttatttg aatacaaaat aaaaagaagt gtataaatat
    87781 aaaagatatc ttttatattt atacacttct aataaattta agaacattat tacattatgt
    87841 atatattctt atttttttaa ttctattttt ttctatactt tctatacaaa atcttatatt
    87901 atcaacctca attacttcat tttcttctgg caatctgctt aggtgtccaa ttataaaacc
    87961 tcctatggaa tcaaactcct cagattccaa gtttacacca ataagttcat taacatcacc
    88021 aattttagta cttccatcta caatatactc atcttctttt ataacttgta tttcatcctc
    88081 ttcttcatca tattcatcct ctatatctcc aacaatgact tctactaaat cttctatagt
    88141 taaaagtcca gatgttccac catactcatc tacaactata gccatctgac ttttttcaag
    88201 tttcatctct tcaagaagtt gtgttatctt tttgaactca tacgtgaaaa aaggttctct
    88261 catataattt ttaatatcaa atgactctat ttcttcatca gataaaaata taatatcttt
    88321 tatattaagt attcctacaa tatcatctat agtttcttca tacacaggca ttctgcttaa
    88381 tttttcagtt ttaaatacct gtattatctc atcataacta tcttccatat ctatagctac
    88441 catatctatt ctttgtacca ttgcattttt agcttgcata tctccaaatt caaaaacatt
    88501 atttatgatt tctctctctt ccatttcaag gacgccttct tcatgactta catttaccat
    88561 agtttttagc tcttcttcag ttatgaatga tttagctcct ttatttgtta taccaaataa
    88621 tttaaatata atatttgtaa ttatattaaa tatccatact attggtctta atataaatat
    88681 aattgctttt ataggtttag atactaatag agaaaccttt tctgaattat ttgctgctat
    88741 tgtttttgga gtaatctcac caaatattaa tactaatact gtcataacag ctgtagcaag
    88801 tgccactcct ttttctccca ttaatcctat aaacaaagat gttgatatgg atgttgctgc
    88861 tatatttacg acgttatttc caaccaatat agaacttaaa agcttatttg gattttctat
    88921 taatgaactt actaacttag ctcctttaac tccttcatct tgcatatatc tgattctgat
    88981 tttactcaac gacattaaag ccgtttcaga tgctgagaaa aatgcagacc ctataagtaa
    89041 tactattaaa aaaataatct gaatcaaatt attgggcgat tccaacactt accacactcc
    89101 tacttacatg tttttatatt aatattaatt ataaataact ttaatattag tttttattat
    89161 atcacaatta catattattt ggaaatatta gataatatat tcaattaaca tatattttct
    89221 aactatattc aatttttact tttgaaccta ttccagcctc actagatgaa actaatagct
    89281 taacaggaac tctattttta agttcctcga catgacttat tatacccaca cttagattat
    89341 tactatgcaa tctttccaat gattctataa caatttctaa aagttcacta tctaatgaac
    89401 caaaaccttc atctaaaaag aaaaattcta atggtgcact tccctttaat tgtatttgag
    89461 atgaaagagc aagtgctaat gatagagatg tcaagaatgt ctctccacca gatagagtat
    89521 caatgcttct tctttctcct ccattaaagt tatctctcat tacaaagttt agtgttgagt
    89581 caatttcaag tgcatatctt ccttttgtta ttccatccaa tcgttttgat gcttccaacg
    89641 ctatatattt taattggttt gtagaaacat attctacaaa tttatttcct tgtacacatt
    89701 tatctaattc ttccaataag tccacattgt gttcaactac cttaagttgt ttattaaaat
    89761 catttatctt atctaaagag tcttttaatg ttattaactg attttgatta gctccaatat
    89821 ctttggatat atttcctatt tctactttca aattataaat ttcatctttt aactgttcaa
    89881 agtactcctt ctttatgctt cttccattta atttttcttt tgatgatttt attttaaatg
    89941 ataataactt ttgttcttct tcatgctcta gtatttcttc aatcaatttt tttactgcat
    90001 cagtatctaa taaagctctt tttactgcat atatactttc aaatttattt tctgctaata
    90061 gtttatttag tgttgatttt tggtctttat attgttcctt agcagtttta agtctaccat
    90121 ctatattgtg cttttcagca atgttttttt catattctaa tctttgctct tccaattttt
    90181 tcttacttga ttcttcttgt tctaatatct tacatatatt ttcttcaaca ttatgtaaaa
    90241 gatttttagc taaatcacct ttcgttatgc taataacttc tttatactta ttatctctac
    90301 tcaatttctt ttctacatat aattctctag cctttattaa ttctatctct atttcatgta
    90361 gtttactctc attttctctt attttaatat ctagtgagtc tctatttttt aataaatttg
    90421 aatgcccagt acttagtaat tctaattctt tttcattctc tcttatttct tttacttttg
    90481 acaatagatt agagacttta gtaatcgtct ttaatcctag gtattcctgt ttaagttttt
    90541 tacatttatt ttctaaacct tctatttcta tgtttaaatc tcttgttaat ttcttataat
    90601 tattcagact ctctcttatt tttaattctt ctttttcaat tttatttttt tcttctttag
    90661 ctaatgttac tttattttca gtactctctt tttctttctc ccactcctga atattagatt
    90721 ttaacaatgc aagttttctt tgttcttcat caagtttttt taataattga ctagatggaa
    90781 tcccccctag ttttcctttt acatcttcaa gttctttagt cttcattttt tctatagata
    90841 aatacccact tactttagca tttaattcct ctatattgtt tcttatactt attttttctt
    90901 tttctaattt ctccagtttt tctttgacaa aagatatatt ttcatcatac ttagttatat
    90961 catgattttc atgatgtttt gacccacaaa ctggacaagg catattttct tttaattcac
    91021 gtctaagttc agaggctaaa tttaaatact ttagttcttc taattctttt tctaagtcat
    91081 ctctgttctt tctattattt tcaagttttt catttagtaa atttacttcc ctttcagtat
    91141 taaacttact ttcaagtatt atctttaatt catcttgtat actgcttttc ttaatttcat
    91201 tttctttagt attatttgct ttatttctta attcagtaac atattcagat tttaaaagta
    91261 aatcagcact cttaccagga cattttttaa gaagaacatc caagtgaagt gataaatttt
    91321 ctaaattacg atttacatca ttcttatctt tatcaatata tctaactttt aaatttatat
    91381 tttctgtctc tttgagtatt tcttctagtt tatttagctt ttgatttttt tcttccaata
    91441 cgttgttgta atctttctcg tattcatatg ctagaaatat tttttgtttt aattctgcac
    91501 ttatatttac tttatcaatc tttccttcaa cttcttttat agatttagta actgcatctt
    91561 ttctggactc tgacacctgc tttacttttt ctaattcaac tttagtctta tttaaattaa
    91621 ttccactttc ttttagctct tttaactctc tatctaataa aactaactct tcttctaatt
    91681 ttattgcttg ttgcaatctt attttttctt cactcaattt agggacttct tcattcttaa
    91741 ttttgtttat tttttcatat ctattttttg taacttcaag ctcctgattt aatattgcta
    91801 atttcttttc taaattactt actgcgaagc tatcttcatc tatctttttt tctagatttt
    91861 gaactgaact tatgtatggg tctacctttt tagcgttact atcattttct acaagagcaa
    91921 tcttttcttt tatctcacta ttttttaaat caagctcatt ttttctaagt tcattttttt
    91981 ctaactttaa ttgttcttca tatactgttt tactttcttc ataagatttt tgagctaggt
    92041 ctaaagcatt atccttatct ttttccaaat tttttaactc tattaattct tggtttatat
    92101 tgttgtatac ttcctctgta attccatcat attgacttaa ttttgatttc aaatctttta
    92161 aattttgtaa ctgtatattt tttctctttt tcactttttc tccaagactt cttccatatt
    92221 tctcaagatt aaaaattctc tctaacatgt ctcttctatc tgcacgtgat aatcttagaa
    92281 actcattaaa tttgtcttgt ggtaaaacta ctgacctagt aaaatcattt gctgttaatc
    92341 ctacaacctg tgctactttt tcatttactt ccacaacttt atcagctaaa acattttggg
    92401 tattgtcatt caatacttct attaatctag cattagaggt ctttgttcct gttttgcttc
    92461 tagcaatggt tctatctaca atgtatcttc ttctagtgtt tttgcttcct atctcaaact
    92521 cgtatgatat aattgcctta tcgcacacac tgtttatata ttcttttgta tttctggata
    92581 tatttccata catagcaata gttattgcat ctaagattgt agattttcca cttccagtag
    92641 ttccaaatat cccaaacaaa cctctttcaa ttaatttttc aaagtctata acttgtttat
    92701 caatatagct attaagccct gtaagttcta atctaattgg tctcatctgc tgattcacct
    92761 tcttcactta ttatatctag aaataaatcc ataagttctc cttttggctc tacaccttta
    92821 ctaaaagaat agaactctct aaacaactct gccatagact tttcttttat atctacactt
    92881 tcttgttcat aacaagatgt tattattggt tttatttcta ttatgtcttt taatagttcc
    92941 ttcatttttt taatttcatt ctgtgatatt atctcatcag tatttatttc aaaatatgac
    93001 caaatctctc tatcttgatt ttgctcacat atatctagtg cttcttctat tccattacat
    93061 ttaaatactt ctataggttt gtaattatta aaatatacat cttcaattat tggtttttca
    93121 ccagctttta tatccactat gtatgcccct ttggtatacg ctctctcatc cttgctatat
    93181 tgaagtggag aaccagaata gtatgcattt aaacgttcgg aagctttttg ttgtttatgt
    93241 aaatgaccta gtgcagtata ttgagccttt tctggcaaat cttttctttc aactaaaaaa
    93301 cttccaccta actgtatagg tctttctgat tcagtactct ctccacctat tacaaatata
    93361 tgtgatacgg ctatatttat agtatcactt ctaaaatttt cttctaaact tctaaatata
    93421 tctcctacct tttcagaata atttttttgt ttctcaaact catcttcact tgaaaaaact
    93481 tcatttaatc ttttttcact tggataaggt aatgtagcaa ccacaatatt ttctccattt
    93541 atattcagtt ttgtacatcc ctgagttgca tatgttattt caaacccttt atacttagct
    93601 tctatcgtag cacttaatgg ataaccatat ataagtatcc cctgttcatg tgctagaggt
    93661 gttattgcag agagtctctc tggattatca tgatttcctg aaattattaa tacacacctc
    93721 tgaccattat tggcaagtct tgatacagtc tgataaaata gtttttctgc ttgtgctggg
    93781 ggattagaag tatcatatac atctccagca attataacca tatctatttc atttgattct
    93841 actattttta taaattcctc acaaaacttt gcttgttcct caattctaga atggccttct
    93901 agggacttcc ctagatgcca atctgatgta tgaataaatc tcattttttc cccttcctct
    93961 atcttttagg tttaatttat atataaaaac taataacaaa cagcttcatc aactccaaat
    94021 aaatatatac atttctcttt tactgactta ccagttaaag tttcaataat atctgcatat
    94081 aaatccaact gttttttata tttctctatt atttgattta tattttcctc atttacaaaa
    94141 tctgttttat aatccaccaa aactatttga ttatcttctt caaaataagc atcaactata
    94201 cctcttaaca tgacactttc attatcataa agttttttat cgtcattgtt tataagcttt
    94261 tcataaatat atatgtcttt catatttact tgtgcataaa ttgatttttc tctatttata
    94321 atttcagcat tcaacattct ttttcctatg ttagaggcaa aaaatttata tattttatat
    94381 ggatttacta tactagcttg tttttctgtt attatacctt ttgaaacaaa acctcttatt
    94441 tgtgatttta tatcatttac actacttaca ttttttaagt ctaaaacctc cataactaaa
    94501 tggactattg tacctctttc tgttccactt attttttctc tttcttcttc attttgaata
    94561 aatagaggct tttttaatat tactttttgt tcaaatatag tatttattaa ctcttcttca
    94621 taattatttt gtatcttttt tatttctgta acagatatag tagcaggttt tcttgtacta
    94681 aattcatatg gatatatata gtttaatttt tcttctattt caccgtaata atcactgtca
    94741 ggattattta catctatttt ttcaagaatt tcttcaatac tttctttttc ttcatcactc
    94801 ttcttttcca ctaaaatatc attcttattc cacaactttc cataccattt tgaattatgt
    94861 tcaacattaa aaactgcgtc taatcctact tcttctagta gattagataa atccctatgt
    94921 cttaatacac aaggcattat ccaatctaaa aaatttttac cttttaatat ctcgtattgt
    94981 gatattgtat ctaaactttc aattccattt gaccatctct ttatactatc ttgtatattt
    95041 ctagtagaac cagttataat caatttttct ttagctcttg taaatgcaac atataaaact
    95101 ctcatctctt ctgataaatt ttctatattt attttgcttt ttaatgcttc tttggctata
    95161 cttggaaaag aaatccttct ctcatagtct acaaattgag gtccataccc taaattatga
    95221 tgatataaaa tactcttttt aaaatcttga gtattgaaat ttttacccat tgcagagcat
    95281 atgactactg gaaattctag acctttactt ttatgaatac tcattattct aacaacattt
    95341 gcattctcac caagtgtttt tgcacttccc atatcagaac tagatttttt taatttctct
    95401 ataaaattta caaagttgaa tattccttta agactggttt cttcaaattg ttttgctctc
    95461 tcaaacaata cttttaaatt agcttgtctt tgacttccac ctggcaatgc tccaacatag
    95521 gcatagtatc cagtccttgt gtacaagtac catataaatt catctgtact catgtacatt
    95581 gacttttctt taaattcctt tagcttaatt aaaaaatctt tacttttgtt tatgcactct
    95641 tcacttggta taaactcaga ctcattttca ttttgagaat ctgtaaatcc atcatactct
    95701 gctgtacttt ttaaaacctc ataaaaaatt ttatctttac tctgaactct aatatcaatc
    95761 aaatcctctg gtgtaaatcc aaatataggt gattttaata cagatataag aggtatatct
    95821 tgcattggat tatctattat ttgaagtaat gacaatattg tttttatttc aatcgtatca
    95881 aaatacccca ctccaacatc agcatacgtt ggtatatcca tattcatcaa ttcatctgca
    95941 aacactggag cccatgcaga tgtagctctt aaaagaataa ctatatctct aaattccaca
    96001 ggtctatatc catctattcc cttatcatag accttttgaa tttttccatc ttcattgact
    96061 ttcattaaat ctttgataat gtttccaacc attctagcct caagttgtat attatctaat
    96121 ttttcttctt cctctatttc attttctttg ttattaattc tatcgtcttt atcatttata
    96181 atatcatcat caagtttatt atctttttgt attaggtgta tctccgttgc tccaccaata
    96241 atagatttct cgtctgtatc cacattaaaa tttgcaccta aatttagtct ttctttttct
    96301 gtatattcaa tttctcctat attctcattc attatatttt caaatatata atttacagca
    96361 tctacaactt cttctctact tctaaagttt ttgtaaagca ttatttttct atgggagctt
    96421 ccctttttat cattatagtt attatatttt tgcaaaaata attctggctt tgcttgtcta
    96481 aatctatata tactctgttt tacatcacca accataaatc tatttggagt ttctgtattt
    96541 gcaacagctt taagcagtac ttcctgcact agattactat cctgatattc atctataaat
    96601 atttcataaa acttgtttct acatccaact gctatatcag aaggtacaat attacctttt
    96661 tcatctacat ctgtaagtat atttaatgca aaatgctcta tatcgttaaa atctattatt
    96721 cctttttctc ttttcttatt gctatattcc tcttcaaatc tcagtacaac actagatatt
    96781 ggtttaacta tgttataaag atattttatt tcttctctaa tagaatcatt atctttattg
    96841 aatgtagcac ttactatact ttctaaagac ttcttagtct tatctcttat agtttttgcc
    96901 ttttcttttg attcttttat atacgaagga gcatcttttg aaattctttt tactcctttt
    96961 acataatttt caaaggacat acttgccatc tttttatatg cctcatccca agatttatta
    97021 catgcttgtg aaatgtcagc aattttttta tattcaacgg ataacttttc tgcaaaagtt
    97081 tctaattctt ctattgattc cacttctttt aatgcccttt ccatatttag acaaagacca
    97141 ttaatctcta tttttacagt gtctaatata gctctagccc aaatagactg tgaaaaatca
    97201 aaattttcat caatattaaa tcgttctgct gagtcaatca accatttttt aggttcagga
    97261 gaagccattg caaatgaata tattcccaag ataatatctt gtacttcttt atctcctcct
    97321 ctttctgcat aactttcaac taaatttaaa aacccctcat ctctttcttc atataaatct
    97381 tcaaatactt cctctattgc ttcttgtttc aaaatagcac attcagtttg gtctcctatt
    97441 ctgaaattag ggtctaagtt tattctatga aagtttgatt ttataacatc caaacagaat
    97501 gagtgtattg ttgtaatact agatttattt aataaaacaa gttggttttg taaatgttta
    97561 ttttctgggt tttcatctaa ggcttttcct atagcatccc ctatcctctc tctcatttct
    97621 gacgcagcag cattagtaaa tgtaacaact aataacttat ctatatcaat tggattttct
    97681 ctacttgtta tcatttgaat tatacgctca actagaacgg ctgtttttcc tgaaccagca
    97741 gcagcagcaa ccaataaatt gcattctcta ctttctataa cttctaattg ctcttttgtc
    97801 catttaggag aactcactaa ttaacatctc ctttcatcat ttttattatc tcttcattac
    97861 tcttattatt taaattttta tacttgttat cttttatagt tgaatcaaac tgacatatag
    97921 cagagtaatc acaaaagtca catgatgttc catctttatg tttatatgga gctatacgta
    97981 tttctccact taacatttct tcacataaat cctttatagc atgccttaca tattttctaa
    98041 gcagtttgaa atcctcatag cttattgctg aagtactttt acctacattt ccatccttag
    98101 taagacctac tggtattact aaagagtttt tcctttctcc atctattaat gatttatcca
    98161 tctctttaac tatgtgagag tcttttacta caagcccctt cattctaagt tcttttaata
    98221 ttgcttcttg tatttcttca tcatctttat cttcattaaa ttttgctatt ggattattta
    98281 ttctacaata tagtattgca gctggattta tatttatatc ttcatccttt gcactttcaa
    98341 ggattgcatc taaatacaca agtaattgta attgtagacc ataatatatt tctgttagac
    98401 ttatggactt attgcctgac ttataatcta ttattcttat atatttattt tctccttctt
    98461 caaattcatc aactctatct atttgtccaa ttaaacttac ttcttgtcca ttttctaaca
    98521 ccatttttat aggtggatat tttccattgt tgccaaattt aacttcataa tctattggtt
    98581 caaatgaacc ttgttttatt tgctgactaa ttatagttat agcagttgtc aacatattct
    98641 ttaatctata tgcaaggtat ttatacctct cagaactatt taatatatat cctggtatct
    98701 ttaaaattat ttcatcaact attataccta tctttaattc tatatatttt tcatcaatat
    98761 ctctccaagt taaattatct tgtaaaagtt cttttgaaaa cctatctaaa atattatgaa
    98821 taaaagttcc taaatctggt ggtgtaaatg aatactcttt acgctttttt gcttttagac
    98881 catattgtat aaagtaagca aatggacatt cagcatattt ttcaagccta gatatgctaa
    98941 gagaattact tttatacaat gaccttattt ttttctcttc aatcttatga acttgattag
    99001 tataactaag accttttata actttttttg ttatagaact atagatttca tcttttaaat
    99061 agtacctata tatatctaac caaatactat taatctcttc tgtattgtaa ccatcactgt
    99121 cataattttt tattacgttt ataagttcat taaatgtagg ggatttaacc gttatttttt
    99181 taagtatatc tttatcagtg cttttatttt cttccactaa ataactttta ttttctatat
    99241 ttggaaatat cttcttaagt cttgatataa ttattgatgg tctaagcgtt ttaccttcat
    99301 ggtctgatat tggatatgtt atggttaaat tcttagatgt cgaagtaaga gctttatata
    99361 caagaaactg ttcttcaaaa ctcctagtct tactatcaat atcaacttcg attcctttgt
    99421 ttccaagact ttccctatca ttatcactca atattccact atcctttgta atcagcggaa
    99481 acacaccatc agtagttcca actaggtata aatatttcgt gtctggattt ttcattctat
    99541 ccacactact tacaagtacc tggtctatac taggaggaac cagtcctaat tcatattcat
    99601 caaaacctaa ggttattaat tttataaatt tttctaaaga aattatttca tcacccataa
    99661 gttccaccat ttggtctaat atgtccacta ctatgtccca aacttgtgag tattgatttg
    99721 caacatcaag ttccccttta tccttaaaat ttactattaa actttctata gtttctggca
    99781 tatttatatc taataaaaat tcatatatat atctacagat ttcttttact ctattttttc
    99841 ctcttaattt ttcttgtaaa attactatag gctctaatac tctatttttt atttcattta
    99901 ttttctcttt taattccagt tcaaattcac tttcttgacc tgaaagactt tgggttattc
    99961 tataatccca tttttcatca aaccattttt tacctttaat cccattagcc aacacataat
   100021 tttctagtaa atttatatca tcattatcaa ttcctataag tccacttttt aaatacctaa
   100081 acatagtttc atacccatat cgtctattct tcatttctaa agctgaaata attaaaataa
   100141 ctattggatt actttttgcc tctctcttct tatctataaa gtttggtata ttatattcat
   100201 taaatataga gtgtactaaa aaatcatatc tatttaaatc tcttgtagca atggttatat
   100261 ctctatatct gacattctta tctctaacta aatgtacaat ttctctggct atctcttcca
   100321 cctctgaata taaattgtta aactctttta tctttatatt ttcagttgga tttgaatata
   100381 ttttatatgg ataagcatta taaaatcttt ctaagtgctg taattcctta actttttcaa
   100441 atctttttat aacacctgtg tttaaattta cttgtggcaa tatttttatg ccttcttcgt
   100501 tgcacaattg agtcaatttt gaataagtaa atttcgttct agaaaacata tcagatttac
   100561 tgtacccaat atagttgata tcatctacag tcaaagagat attaacactt ttagacttat
   100621 ttaatatact ctttatgaca ttgtactgat ttggtgtaaa acctgtaaat tcatctatat
   100681 aaacacaagc tccatctaaa taactgctta attcaatttt acttgccaat gaagttaaca
   100741 tatcttgagc atctacataa ttttcatgca atttcccttc aaaagaatta tatattttac
   100801 ttatatcttt taattttaaa cttaaagttt cattatccaa ttctccagat atattttcta
   100861 acatttcagg gcttatattg tattgtttca tttctgatat catatctgta attgaactta
   100921 caaaaccaga ctgcgatttt gattttgaaa acacatttaa ttcttcactt acatcttcaa
   100981 tagctctata taccatcatt gcttttccac tagaatttat atttacatct gtcaatcctc
   101041 ctacttgaga aaaaactata ttacttagtg tcttaaagct aagtacccta actcttagat
   101101 acttatcttt ttcttttcct aaaaatagtt tacttactct attttcagcc tcaaatgtgt
   101161 actgttctgg aactaaaagt ataattgatg tcgtttcatt tttttgagct tcttttttaa
   101221 tttcatctag tatataggtg gattttccac ttccacttct acctaataca aacctaagtc
   101281 ccataaattt ccgcctccaa aatactataa tctatttctt tgccattaca taacttatta
   101341 aattcacaat acttgcaatg ctttttattt ctatagcaca tacgttcttc tttattatat
   101401 accttttcta tatcattgta tagattgtct tcgtatgtat gttcttcata attagtgttt
   101461 tttatcatac ttatataatt ttgaatttta tttctatttg cttcatgttt ttcttcatca
   101521 tataaaatgg taattggtaa gtcatcaaat tcaggttgat aatatctcat atttatatct
   101581 ttaacattta tgtccatttt taaaacttta ggtattactt cttttgctaa aaacatataa
   101641 acaatagttt gtattctatt ttctacgtgt ttatagtcaa tctttttaga ctcggttttc
   101701 caatcccata tttctatatt gttatttcca attacaacta aatcatactt tgcctgtatt
   101761 atatccccat ttaatgtcat ccttacttca tattctggca aatacgtttt tcctttttca
   101821 ataggaacaa ctttttttat attttccatc cactttaaaa actttttact atttttatca
   101881 tcctcattaa aatagattcc aagaggtata ttgctaaaat atctttcaca aatcaaatga
   101941 aaatctcttc ctgtttttaa agtttcataa tactctctac taccaacatc atcatttttc
   102001 caatttattc tatcaatata tttatattta aatttcaatg gacatgattt atatgtgtta
   102061 attgaatttt gactataaga aaaatatttt aatttatcgt tcactcatat cacttctctc
   102121 ttaactttat tttttagtag ttacttactg tacatattgt tttatcccct tttagatata
   102181 tttcaagcaa tcttttaata acttattata tattaatttg accttttttc aataatatgt
   102241 tgattaagtc tgcttaaata tatacatggt ttttgctctt ttctcttatt ccctatatca
   102301 ctttcatctc tataagcact tcctgaaagt actaacattt cctttgctct agtaatccct
   102361 acatataaaa gtctaatctt ttcatttata gaatcaatct tcgttttatg agcataatct
   102421 gttgaaatac ttccttttaa tatagcttca acttctgatt taataattgc catagggttt
   102481 ttatattttt cttttagata ccatttatca ctctgaaact tttgattaat attgtcaggg
   102541 aaattatatt caacaagacc tagtaaaaac acacaatccc attccatccc ctttgattta
   102601 tgatagttac atagtgttat actaccgggc tctggttcat atccatttat ctcatatacc
   102661 acatctatta tatgattaaa caccctgttc tttacatcaa acaataaatt atatacatct
   102721 tctaaatcca tatttatatt ttctgatact agatacttta tataaaaaga tatataatcc
   102781 agtacagctt tatcttcttt ttctaaatta agttttttcc caataaacaa aactaagagg
   102841 tctaacctta ttattggata ttctaatatc tgttttataa tatctattcc tcttttaaaa
   102901 cttaaatata tgtcactttc ttctttaaat tctattaaat cattatctaa cttactaaat
   102961 tcaaaattat aaataatttc ttcagtagaa tagtttttta attcttctaa aaaatacttt
   103021 atttcttctt cattatcagt atctataaaa actttattaa gtacaactat taacttttct
   103081 acatcatcac aatgtaacat aaaatcaata atatatgcta tgttgtttat aaccttcctt
   103141 ttattcaaag aatttggtcc taattcttca aaagttagat tattctctct aagttcttta
   103201 gcaacttgtg tcacttgttc attaaatgga accaatatac ctatactttt atctggatat
   103261 ttatttttta tacctttaac atatctagca gtttgctcta tttcaatttt ccaactctca
   103321 taccacttta catttatatt ataatgttct ggattaggat tttctttata tcccattcca
   103381 tttggcacag tttttatttg catatcttca agagcatgtc tacattcttt ctgtctaaat
   103441 tcctttgtaa catacttgac aagagtgttt gctaaatcaa gtatatcttt tgaacttcga
   103501 tttgacatat ccatcctata acagaaatct gcactattta taaattcttt aaaaaattta
   103561 gggtctgatg atgaaaatgt tcctgttata ctctgattta tatctccaac tctaactagg
   103621 tttttatttt cgctagatat aatttttatt atattacctt gaatttcatt tgagtcctga
   103681 cactcatctt caaatatata tttgtatcta gtttgaaact tctttctaag cccttcgtct
   103741 aagcttaaag ttttttctgc taaaattaat atatcatcat aatctagcaa tccattttgc
   103801 tttaattttc tatcatacaa tttatatatt ggtaaaataa ttttaagcat tcctttgtgt
   103861 cctcttgaca atatttcttc aaggttatct ggagaaattc ccttatactt aagttcactt
   103921 attgcatttc ctgcaaattc aaaaaaacct ctttcccagg cttcaagagt tatttctttc
   103981 cagcgttcat ctttttgctc ctttaaaaac catctaaaag ctctttctcc accttcaaat
   104041 ctaaatctac ttatacattc atttaatata atactttttt gcaaatcgtc agctatgtta
   104101 aaatcctcac ttaacattac aacttctggc ttttccttta ttatttttac agccaaactg
   104161 tgtatagtca ttacttcata cgaattagta tcatctattt tattctcttc aagaattttt
   104221 cttattctac ctttaaaatt atttacagca ctattcatat atgtaagaat taatatttta
   104281 ccacccttat gtttctgctc aagtaatagt ttagctacta aatttgttat tataaatgtt
   104341 tttccagcac ctggtactgc tggtactgcc atagtaccat tatcgtaatt tattattggt
   104401 atctggtctt ctctataatt tatattaatc ataattttta tctcctttat gtgagatttt
   104461 taaaagtata ctatataata tactctcttg gatataacca ttaatcgcat attcactttt
   104521 aaatgcatag attttctttg catttaataa caaattatat actgtattgt atagatagta
   104581 ttttttataa tattcttcca tttcatttgt ataaatcttt ttttcttcaa aagattttct
   104641 aagtacaatt aaattactta tatctttttc tattttcata ttccaagcat tacttcctat
   104701 atctacccat atttgtatac ttctatctat atttgatgat atatatgaat aaggagtagt
   104761 tattactatt ttgtctttgg atttcaactc ttctatatcg acaacagagt aataatcgtt
   104821 tattgtactt tttaaagctt ctataaatat cttttctttt ttcatcccaa gtagacttaa
   104881 actttcacta aaagactcac tttcagctat tatcttttta cataacccaa cattcttttt
   104941 accttctttt aaatttaaca ttttatctat gtaaaattga gttaaaaatt catgtatttt
   105001 tatatcactc ttcttttttt cttcaatata ctgtaaaatc tcacgatact gactatgttc
   105061 atctatatta tcaatactta aatgtcttgt ttcattaaaa atcttaaatg cttttatcct
   105121 atttatttct aaaagagttt ctaaaaagct taagtattca tcatctttta taaaatctaa
   105181 atatccataa aatatacatg tagccactac caatgcattc ccatatggat aatccacagc
   105241 ttttttatcc ttcttagtac taaaaacatc tatatttttt tctatcaaag aatctctaat
   105301 ttgatgctcc aatatactat tgtttatagg agatataatt gctatatctc ttggagatac
   105361 atcttcttct ataagagata taactttttc acttattaaa tctaacattt cattatataa
   105421 ttgacttgat tgatttaatt ctatatttgc aggtaataag tataaatctt ctattctaat
   105481 ctccttcttt ttaatatttt cttctttagg cttaattttt gatattattt tctcatttat
   105541 atagtctata tccacattat taaatacaga ataatcccta ctataatcaa aatatatata
   105601 actttgtaat gtattatcca atgcttctgt tataaaatca acttctgcat tacttgagct
   105661 ttcaaggcta tccacaatta tatatctcac ttctgtaaat aagtttttta aataaaaatc
   105721 atcattaagt aggtattgat tgtataaata tatgcttaat gcattatcta aaattgaatt
   105781 tgatagtaag gaatttatat aatatgatat aatttcatcc atttgagtgt atgaaaatct
   105841 atatatacta tctttatttt tctttgatga atatattttt tctccaataa aatcaaaatc
   105901 aattaaatta aatgcagact tatttatgtt agtcattata ctatttgcta tggatttatt
   105961 ggttcctgta atatcttcaa aatatccttc ttgtatcctt ttttccttaa ctttttgcac
   106021 tattaaatat tcacttaaac tatttgatat aaatgttgga gatacaacct ttgtacttat
   106081 ttcatcacat ttttctatta ttagtggcca gtatttaact aattctttct tcacaaaact
   106141 tatatatgtt gttattttca gttcttcact ataatttaat cttagagacc tttcatatcg
   106201 tatctttgtg ttattgtttg gtactaacaa aagaatacac cttgaattat taccagtttt
   106261 atctaataat tctctatatt tatctaaagc ttcatttcca tattctttta aactactttt
   106321 ttcaaataaa tattccatat aatcaccttc ctcaattaaa ttaagtgtat attatctaat
   106381 tatatcatat tgtgtatttt acaaaacaca ttcaaattat tgcatttaac tacaaaaaaa
   106441 gactgtatat atacttgcat tcacacgtgc aaatgtacat acagtcttac tattgataca
   106501 cttatatttc attttttgac tttagtataa atttacaact ataaatttac attaagtttt
   106561 taaatatttt tatttccatt tattttaatg aatcttctat tcctttacct atttgtgtac
   106621 ccatttcttt tacttttgca gatgagttta ttaaactcat atcattttca ttatccaaaa
   106681 atgcacattc tacaagtaca gctggcatat cattacttct taaaacgtat aaataatctt
   106741 ttgtagaatc actaggaagt actcttgttt ttatacctct atttgtaagt ttaaatttct
   106801 ctaaaattct atttaatata ttggtagcta cagttttagt agtccctcca tttttatctt
   106861 taagcttata aaatacttct acaccatgac cttgtttatt agttgagcca ttataatgta
   106921 tacttgtaaa taaatctggt ttcaatgaat tagatagagc agttctattt ccaagagata
   106981 aagtcttatc tgtgtctctt gtcattatta cattgaatcc ctttgaacgt aaatattcag
   107041 ttgttgcaag tgaagtattt aaattgtaat ctttttcaat cattcctcca gatgaatttc
   107101 caggtgctga accaccatgc cctgggtcaa tcataactgt cttgccccca ctatttcctg
   107161 gttctgttgg agtcgtattg tgtttagata atgatgatgc tatagagctc attaccttag
   107221 aagtcaatcc tcctcctatt ttgtatacct tattagctga tttagcttct aaattatctt
   107281 tatggtatgc agatacatac gtctttggtg ttataagtat tggagacttt aggttcgcag
   107341 ccaatggacc tgctgctaaa gcatcaacaa gtacatctga tttagctact aaaacagcct
   107401 ctaactcatc atctgtatag aattttttta ttacgtttgc atttgtttcg tgtctatctg
   107461 ctccgtatac tctattatta gtaacactat ctttagttat tccatttacc ttatttataa
   107521 catttgttga tatcatttga ggaccaccta taaaataagc attttgtaaa tcctcactct
   107581 ttaaccattt atatgtattg tctgtaatac tatctttatc agttaatata attggttgct
   107641 tgtcttgacc tgcttttgct gctatagtaa gtgcatccac ttctccgcca ttagcatttg
   107701 ttatgtatac tttttccaca tcatgatttt tatctatttc ctttgctatc aataaagatg
   107761 tctcatatct attagaacca tttaaacgtg tttgactagc atttacagtt gatttaattt
   107821 gattagcagt agttttagaa atagcattct catctccaat tataattata tctttagggt
   107881 ttaggcgctt taattctgat tttacactat taggtacatt gtttttttca accaaaagta
   107941 ttggtgcatt atatgtggtt gccagtggag ttgatataat gccatctata cttacatccc
   108001 catttattat aactacctta tctgaaccat ttttccagcc ctctttactt attttaactg
   108061 ctgtttcata tctatcactt cctgtaagtt ctgtaatagg tgaagaagtt tttctcaaat
   108121 atttatttac atcttttcta gaagttatat cttcaattat acattcctgt gttacattat
   108181 agtcttttaa atttaatccc agcttttcta ggtcttgtct gtttaaagtt ttaggattat
   108241 ttttgctgtt tattgttatc ccttgtagtt cccaaacatt tagatattct gggtcttggt
   108301 tataattgat agttccttca tatgccccag ttgattgatt gtaatttaga gttgtattca
   108361 atggcatgtc atagcattta tactttaatg taatgctatc tgcatctggt ttttctttaa
   108421 atttgacact aacttttgat ttgctaccta aaacgatact ttttttctct aagtttatat
   108481 ttaaatcagc gttattttcc atagcattag attcagtagc aaatgccatt gtaggtgcga
   108541 cgattgccat tgaaagaatc cctgttgtta ataattttgt atttcttctc atattaatct
   108601 ccttacttaa cataaatttc tactttattt ttaatctttt aacttttgaa aatatgtcaa
   108661 agtgtatcta acccaaaata taaataatat ataagtaata tataaaaata aacttataaa
   108721 actcaaaaaa taaaaaaatt aaatatattt aatacattta attatctata ctatatcatt
   108781 tattgacata aaaatccaca aaaaacacac aaacacacaa taaaatatag taattaagct
   108841 tttattgtgt attacaaatg aatactccaa ctcttgacaa ttaactgtag ccttaagata
   108901 tgtaaattct tcatataata taaatccttt tacaattaat cataaaaaga ctgtatatac
   108961 ctcatttcgc acttatatgc acaatgtata tacagtcttt attactatta taattacatt
   109021 cttatcttat agctttagtt taaatttaaa attgcagatt aatttatttt aaatttttct
   109081 atgctctcat tgtagactat tttaatgaat cttctatgcc tttacctatt tgtgtaccca
   109141 tttcttttac tttatttgat gtattaagta aactcatatc tttctcatta tccaaaaatg
   109201 cacattctac aagtacagct ggcatatcat tatttcttaa aacatataaa taatcttttg
   109261 tagggtcagt tgatagtgtt cttgtttttg cacctctatt ttttagatta aatttctcta
   109321 agattctatt taatatattt gtagctacag ttttagtagt ccctccatct ttatctttaa
   109381 gcttataaaa tacttctaca ccattcccag ttgtatcaga tgcattatag tgtatactcg
   109441 taaataaatc tggtctcaaa gagttagata aagcagttct atttccaaga gataaagtct
   109501 tatctgtgtc tcttgtcatt attacattga atccctttga acgtaaatat tcagttgtcg
   109561 caagtgaagt atttaatgta tagtcttttt ctctaatacc ccctaatggt ttacctgttg
   109621 ttccagtgtc cgaaccacca tgtcctgggt caatcataac tgtcttgccc ccactatttc
   109681 ctggttctgt tggagttgta ttgtgtttag ataatgatga tgctatagag ctcattacct
   109741 tagaagtcaa tcctccacct attttatata ccttatttgc cgatttagct tctaagtttt
   109801 ctttatggta tgcagataca tatgtctttg gtgttataag tattggagat tttaagttcg
   109861 cagccaatgg acctgctgct aaagcatcaa caagtacatc tgatttagct actaaaacag
   109921 cctctaactc atcatctgta tagaattttt ttattacgtt tgcatttgtt tcgtgtctat
   109981 ctgctccata tactctatta ttagtaacat tatctttagt tatatcattt accttattta
   110041 taacatttgt tgatatcatt tgagggccac ctataaaata agcattttgt aaatcctcac
   110101 tctttaacca tttatatgtg ttgtctgtaa tactattttt atcagttaat ataattggtt
   110161 gcttgtcttg acctgctttt gctgctatag taagtgcatc cacttctccg ccattagcat
   110221 ttgttatgta tactttttcc acatcatgat ttttatctat ttcctttgct atcaataaag
   110281 atgtctcata tctattagag ccctttagac gtgtttgact agcatttaca gtcgatttaa
   110341 tttgattagc agtagtttta gaaatagcat tctcatctcc aattataatt acatctctag
   110401 gatttaaacg ctttaattct gattttacac tattaggtac attgtttttt tcaaccaaaa
   110461 gtattggcgc attatatgta gttgctagtg gagttgatat aatgccatct atacttacat
   110521 ccccatttat tataactacc ttatctgaac catttttcca gccttcttta cttattttaa
   110581 ctgctgtttc gtatctatca cttcctgtaa gttctgtaat aggtgcagaa gtttttctta
   110641 aatatttatt tacatctttt ctagaagtta tatcttcaat tatacattcc tgcgttacat
   110701 tatagtcttt taaatttaat cccatctttt ctagttcttg tttgtttaaa gttttaggat
   110761 tatttttgct gtttattgtt atcccttgta gttcccaaac atttagatat tctgggtctt
   110821 tattataatt gatagttcct tcatatgact cagttgattg attatagttt aaagttgtat
   110881 ccaatggcat atcatagcat ttatacttta atgtaatgct atctgcatct ggtttttctt
   110941 taaacttaac actgactttt gaggtactac ctaaaacgat actttttttc tctaagttta
   111001 tatttaaatc agcgttattt tccatagcat tagattcagt agcaaatgcc attgtaggtg
   111061 cgacgattgc cattgaaaga atccctgttg ttaataattt tgtatttctt ctcatattaa
   111121 tctccttatt taacataaat ttctactttg ttttttaacc ttttaatttt tgaaattata
//
LOCUS       DAMJVK010000002        90658 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.002, whole genome shotgun sequence.
ACCESSION   DAMJVK010000002 DAMJVK010000000
VERSION     DAMJVK010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 90658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 90658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..90658
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.002"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(121..894)
                     /locus_tag="PC414_000103"
     CDS             complement(121..894)
                     /locus_tag="PC414_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HDF2462523.1"
                     /translation="MKNVANATHYLNMDTKELFNLYNKNKNVDIRNILIERHLYLARL
                     LAKKYINKGVDFEDIYQVASLALIYAIDRYDVEKGFEFSSFATPTIVGEIKKYFRDKV
                     WTLRVPRRIQELSKKISDAKIKLEQENKKHPKVKDIADYIGVSEEDVLEAMEASYGYQ
                     PMSLDSSSNDDSEDKDITLIDKIGKEEGNFGSIEYEDFINKFIETLNELEVKIFKDRF
                     FFDKTQSSIAKELGISQMTVSRLEKKVVEKLKKEYEKNL"
     gene            complement(894..1280)
                     /locus_tag="PC414_000104"
     CDS             complement(894..1280)
                     /locus_tag="PC414_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2462524.1"
                     /translation="MEITTNPDYVSIIRLTASGIANKMGFPIDDIEDIKVAVSEACTN
                     AIKHSKDDVFNIVFNILDNAINIEIQDNGKGYDVSSISTPDLENPKESGLGLFIIKTL
                     MDDVDIESEHNQGTKIKMTKYLGVDI"
     gene            complement(1305..1622)
                     /locus_tag="PC414_000105"
     CDS             complement(1305..1622)
                     /locus_tag="PC414_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HDF2462525.1"
                     /translation="MSMNIDSNLDSQNKFWNVCLDGELDVSTADKLKEHLHALIEKNM
                     LDVKINLKDLDYIDSTGLGAMIGVLKKLKINEKEIYIVNPKSNVRKIFTITGLDKIFK
                     VEG"
     gene            complement(1634..1894)
                     /locus_tag="PC414_000106"
     CDS             complement(1634..1894)
                     /locus_tag="PC414_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HDF2462526.1"
                     /translation="MNAWGWQIGAVLVGSSALIVAIYLAKTLNSINKVVEKAYKIVDY
                     NERHIQDIVENASSISKGIDDIVYVINKILSIGNIFKIIKRK"
     gene            complement(1894..2190)
                     /locus_tag="PC414_000107"
     CDS             complement(1894..2190)
                     /locus_tag="PC414_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDF2462527.1"
                     /translation="MCNKRSCKKGGFLLLGAILGFIFGMFFAPKKGSELRKETKEKFN
                     DVKENPKEVLHETFNDVKERIINLVDDDNNEEDIKISEEDIVISKSFDDEGDVN"
     gene            complement(2259..4685)
                     /gene="gyrA"
                     /locus_tag="PC414_000108"
     CDS             complement(2259..4685)
                     /gene="gyrA"
                     /locus_tag="PC414_000108"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HDF2462528.1"
                     /translation="MEENNKILPIEIAEEMKKSYIDYSMSVIAGRALPDVRDGLKPVH
                     RRILYSMSELNLTPDKPYRKSARIVGDVLGKYHPHGDTAVYYAMVRMAQDFSTRALLV
                     DGHGNFGSVDGDSPAAMRYTEAKMSKLSLELLRDIEKETVDFKPNFDESLKEPSVLPA
                     RYPNLLVNGSNGIAVGMATSIPPHNLAEVIDATVYLIDNPECSVDDLIKFVQGPDFPT
                     AAIIMGKESIAEAYRTGRGKVKVRSRAFIEELPKGKQQIIVTEIPYQVNKAKLVERIA
                     ELVKEKRIEGISDLRDESNRNGMRIVIELKRDANANIVLNNLYKHSQMEDTFSIIMLA
                     LVDGQPRVLNLKQILYHYIKHQEDVVTRRTKFELNKAEARAHILEGLKIALDNIDAVI
                     SLIRASKTGQEAKLGLIEKFKLTEIQAQAILDMRLQRLTGLERDKIEAEYEDLIKKIN
                     RLKEILADERLLLNVIKDEITIIKENYSDERRTEIRHAEGEIDMRDLISDEEIAITLT
                     HFGYIKRLPSDTYKSQKRGGRGISALTTREEDFVRHLVTTTTHSRLLFFTNKGRVFKL
                     NAYEIPEGKRQAKGTAIVNLLQLSADEKIATLIPIDGNDENEYLLLATKKGIVKKTKR
                     EEFKNINKSGLIAIGLRDDDELIGVELTDGKQEVLLVTKEGMSIRFDENDIRYMGRTA
                     MGVKGITLSKEDFVVSMNLCSKGTDVLVVSKNGFGKRTNIEEYRSQIRAGKGIKTYNI
                     SEKTGTIVGADMVNEDDEIMIINSDGVLIRIRVNEISLFGRVTSGVKLMKTNDEVNVV
                     SIAKINIEEE"
     gene            complement(4705..6606)
                     /gene="gyrB"
                     /locus_tag="PC414_000109"
     CDS             complement(4705..6606)
                     /gene="gyrB"
                     /locus_tag="PC414_000109"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003226808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HDF2462529.1"
                     /translation="MKQEYGASQIQVLEGLEAVRKRPGMYIGSTSPRGLHHLVYEVVD
                     NSIDEALQGYCSDIYVSINEDGSVLVKDNGRGIPVEIHPKTGKSTLETVLTNLHAGGK
                     FGGGGYKVSGGLHGVGVSVVNALSKWMVAEVYLNGKIYKQTYEKGLPTSKLEVVGESQ
                     DKGTMIQFMPDETIFDEIEFKYETLEYRLRELSFLNKGIKIVFEDKREGQEKRKEFHY
                     TGGLVEYIKYLNKSRTGIHDDIVYIDKKVDDCFVELAMQYTDGYTENIYSFANNINTH
                     EGGSHLSGFKAALTKTVNDYAKRNKFLKENDVNLLGEDIREGLTAVVSVKLPEPQFEG
                     QTKTKLGNSFMRGIVDSVTVDELGSFLEENPSTARIIVDKALRAQRAREAAKKARELT
                     RRKSVLESTSLPGKLADCAEKDPSKSEIFLVEGDSAGGSAKQGRDRNSQAILPLRGKI
                     LNVEKSRLDRILSSDEIKNMITAYGCGIGEDFDIDKARYHKIIIMTDADVDGAHIRTL
                     LLTFFFRYMRPLIDEGYVYAAQPPLYKVTKQKKEHYVYSDKELNILLDEIGRNGVELQ
                     RYKGLGEMNAEQLWETTMNPETRTLLQVTVEDAAIADEVFSMLMGDKVAPRKEFIEEN
                     ARFVRNLDI"
     gene            complement(6607..7722)
                     /gene="recF"
                     /locus_tag="PC414_000110"
     CDS             complement(6607..7722)
                     /gene="recF"
                     /locus_tag="PC414_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HDF2462530.1"
                     /translation="MKLKSLQLVNFRNYKKLHLEFNGKVNLLVGKNGQGKTNIVESIY
                     MLSFGKSFRTNKDKEMVRFNSENLYIGGSFSKYNKYSLIELIIGKDKKGIRINKVPLQ
                     KIQELLGNLNVVIFSPEDLRLVKEGPKERRAFIDKEISQIIPKYYKYLTNYNKTLSQR
                     SRVLKNIHVDEALLDVYDDTLAKYGSYIYILRRDFIKKIANISENMHMNLTNGVERLS
                     IRYKNQINITDEDTIDTVYNKFLAKLSSNRPNDIESKTTRYGIHKDDLNIFINDLDAR
                     LFGSQGQQRTASISLKLSEIELIKNEVEEYPVLILDDVFSELDEARQKLLVNNLSNVQ
                     MFITSAEVSHKKIFDEKNVTIFNIENGDVISIENGGN"
     gene            complement(7740..7946)
                     /gene="yaaA"
                     /locus_tag="PC414_000111"
     CDS             complement(7740..7946)
                     /gene="yaaA"
                     /locus_tag="PC414_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HDF2462531.1"
                     /translation="MTEITIESEYIKLDQFLKLAEIASTGGHAKFLIQEGLVTVNDEI
                     ELRRGKKIKSGDIVEIEGTKIKVL"
     gene            complement(8083..9189)
                     /locus_tag="PC414_000112"
     CDS             complement(8083..9189)
                     /locus_tag="PC414_000112"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HDF2462532.1"
                     /translation="MKIICNQKILANRIGIAQKAINGKTTIELLKGILISTEEGQLKL
                     TGYDAEIGIETYVQAEIIEKGDVVVDARLFGDIIRKLPDSFVEIETDSENNIYINCVN
                     SRFKIKGYAAKEFPKLPELNEEDLYSIPQEILKNMIKQTVFAISQDQTKPVLMGELLE
                     IVDRNLNLVAIDGYRLAVKSCSVDSLTENIKVIIPGKTLIDVNSLLSGEDNVKVGFNE
                     KNAIFIINDTKIITRLLEGDFIDYKKLLPREHNSRVKLNTKELLNSIERASLLSQSEK
                     NNLIKLSIRDKVMAITSNTEKGNVYEEVEIDLDGDYLDIAFNSRYFIEGLKNIDNEEI
                     FIEFTTNVNPCIIKPTDDVNYIYLLLPVRISSNI"
     gene            complement(9431..10753)
                     /gene="dnaA"
                     /locus_tag="PC414_000113"
     CDS             complement(9431..10753)
                     /gene="dnaA"
                     /locus_tag="PC414_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HDF2462533.1"
                     /translation="MMDIVSLWDKTLQLIKGDLTSVSFNTFFKNIVPLKIHLNDLILL
                     APSDFNKDILENRYLHLIEDAISQLSLKKYNIKFVLSEKEVADLNSDSTDLNYRVLYP
                     NLNPKYTFDTFVIGNSNRFAHAACVAVAESPAKAYNPLFLYGGVGLGKTHLMHAIGHH
                     IVSQKKDSKVVYVSSEKFTNELINSIKDDKNEEFRNKYRNVDVLLIDDIQFIAGKERT
                     QEEFFHTFNTLHEANKQIIISSDRPPKDIPTLEDRLRSRFEMGLITDIQAPDFETRIA
                     ILRKKAQLERIDVPNEVMSYIAKNIKSNIRELEGALTRVVAYSSLSNRVISFDLATEA
                     LKDIITTSKNEEINVLRIKEKVSSVFNLKMEDFNSKKRTRSIAYPRQIAMYLTRELTD
                     LSLPKIGEEFGGRDHTTVIHAHDKVSKDIEESEEIKTKIDKIISDLKG"
     gene            11409..11546
                     /gene="rpmH"
                     /locus_tag="PC414_000114"
     CDS             11409..11546
                     /gene="rpmH"
                     /locus_tag="PC414_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDF2462534.1"
                     /translation="MSKRTYQPKKRQRSKEHGFRKRMKTSNGRNVLKRRRAKGRNRLT
                     H"
     gene            11624..11968
                     /gene="rnpA"
                     /locus_tag="PC414_000115"
     CDS             11624..11968
                     /gene="rnpA"
                     /locus_tag="PC414_000115"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HDF2462535.1"
                     /translation="MDFNRTKGLKKDSDFRKVYKHGKSFANKYLVIYILKNKSDYSRV
                     GISVSKKVGKAITRNRVRRLIKEAYRLNIDEKIKPGYDIVFIARVSSKDATFKDIDKS
                     IKNLVKRTDISI"
     gene            12000..12251
                     /gene="yidD"
                     /locus_tag="PC414_000116"
     CDS             12000..12251
                     /gene="yidD"
                     /locus_tag="PC414_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDF2462536.1"
                     /translation="MNKIIYILKEISKYLSNLCIYLVRFYQKYISPLKGPTCRFYPTC
                     SQYSIEAFKKYGFVKGMYLTIRRILKCHPFHPGGYDPLK"
     gene            12283..12990
                     /locus_tag="PC414_000117"
     CDS             12283..12990
                     /locus_tag="PC414_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDF2462537.1"
                     /translation="MNIIGNFLGLILKVIFEFVNHYGISIILFTILVKIILLPLTIKQ
                     TKSTKAMQDIQPRIKEIQEKYKNKPEKQNEEIVKLYGEAKINPLSGCLPLLIQFPILI
                     GLFSVLREPVAHGVFANKAAFLAADNGFLWIKSLTSPDYVLAVFSGASAYVMQKVMTP
                     KDQLQGSMKVMTYVMAGMSFYWGFIFPAGLTLYWTVSNLFSIAQYYLIMNPLKAKLAA
                     NSKEEKVNEKKPKINKK"
     gene            12959..13588
                     /locus_tag="PC414_000118"
     CDS             12959..13588
                     /locus_tag="PC414_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HDF2462538.1"
                     /translation="MRKSLKLIKSKSKEEAISKAIAELNLKAEDIEVEILENPSKGFL
                     GFIGAKDGTYEIFVIEREVDVAKNFIEVMLKNANVDAKVNVSQKDNLIMVDIEGKEAA
                     SLIGRRGETLDSIQFLTGLALNKINKDSHTRVLVDIENYRSKREESLIRYANKVAREV
                     AKTRKTKKLDYMNPYERRIIHSALQNDKYVITYSEGTDPYRRLVIECKR"
     gene            13700..15079
                     /gene="mnmE"
                     /locus_tag="PC414_000119"
     CDS             13700..15079
                     /gene="mnmE"
                     /locus_tag="PC414_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HDF2462539.1"
                     /translation="MFIDDTIAAIATAPGEGGIGILRISGEKALKVAEEIFKSMSGKS
                     IEEYNKRTLIYGNIVDNENIIDEVLLAYMKGPNSYTGEDVIEINCHGGFISVKKILEL
                     ILSKDVRLAEAGEFTKRAFLNGRIDLSQAEAVIDVIKAKTDIAHEVAQNQLEGSLSKK
                     IRELRDKVTEILAHVEVAIDYPEEDIEHITYQTLKEKTDELKKDIKKLYDTAESGKIL
                     REGLKTVIVGKPNVGKSSLLNSILGENRAIVTDIPGTTRDVIEEFVNIKGIPLKIVDT
                     AGIRDTDDIVEKIGVEKSKESFTSADLIVMVLDASRKLSEEDIEILEKLKDKQTIVLL
                     NKNDLKQEIEEEKILKYVENNSIIKISALQQEGIEELQDKIESMVYKGSIKNNSSLVV
                     TNSRHKDALSKAYKSATDALIALEQSMPFDFVEVDLKNIWDYLGYINGDTVTEDLLDN
                     IFHNFCIGK"
     gene            15177..17069
                     /gene="mnmG"
                     /locus_tag="PC414_000120"
     CDS             15177..17069
                     /gene="mnmG"
                     /locus_tag="PC414_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HDF2462540.1"
                     /translation="MKFEAGKYDVIVVGAGHAGCEAALATARMGYKTLIITMSLDSIA
                     LMPCNPSIGGTGKGQLVKEIDALGGQMGLNIDKTYIQSRMLNTAKGPAVHSLRAQADK
                     FKYHEEMKKTLEDEPNLDIAMDEVVEILHEGNVVIGVGTKLGCSFKSKAVILATGVYL
                     NSKIYMGEVAFYEGPNALGYAKYLTDSLVELGLRMRRFKTGTPARVHRDSIDFSVMSL
                     QEGDEKVTPFSFMNENIEKKQEPCYLTRTTEETQKVILDNLKRSAMYSGVIESTGPRY
                     CPSIEDKVVRFSDKTSHQLFIEPEGLNTKEMYIQGISTSLPFEVQLDMYKTIKGLENC
                     KIMRPAYAIEYDCVDPTQLKISLEIKGVENLFSAGQFNGTSGYEEAAAQGLMAGINAV
                     RKIEGKEPFVLDRSEAYIGVLLDDLVTKGTNEPYRMMTSRAEYRLYLRQDNADMRLTQ
                     KGYDIGLVKKDRYERFLNKKAAVEKEFERLKNERVTPKEVNSLLEEKGATPIKVGISL
                     YEFLKRPEVTYELLEELGKGAGEDVSREVKEQCVIITKYEGYIEKQLKQIDQFKKLEN
                     KKLDEKINYSSIEGLRLEARQKLDDIKPISIGQASRISGVSPADISVLLIYLEQIRRT
                     RGGKGE"
     gene            17062..17781
                     /gene="rsmG"
                     /locus_tag="PC414_000121"
     CDS             17062..17781
                     /gene="rsmG"
                     /locus_tag="PC414_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDF2462541.1"
                     /translation="MNNIELLKNGIEGFNIDTNDSMLEKFKIYREILVDWNKKMNLTG
                     IEDEKEVYIKHFLDSISAVKNGYIKNGMSIIDVGTGAGFPGIPLKICLDSLELTLLDS
                     LNKRINFLEEVSRVLELDNITFIHGRAEDFGKDEDYREKYDVATARAVAGLPILMEFC
                     VPFVKVGGYFICLKGPNANLELEESQKAIDVLGLEYIEKIDVELPEVDLNHNILVFKK
                     VMETPVKYPRKAGKPAKNPIK"
     gene            17944..18729
                     /gene="noc"
                     /locus_tag="PC414_000122"
     CDS             17944..18729
                     /gene="noc"
                     /locus_tag="PC414_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HDF2462542.1"
                     /translation="MEDKRVMEIPIEDVVPNPYQPRKIFSQVSLEELSNSIKVYGIIQ
                     PITVRAKDGKYELIAGERRLRAAKLAELKTIPAIINNMNDESSAVLALLENLQREDLN
                     FIEEAIGYENLIKEHAFTQQQLAEKLGKNQSTVANKLRILKLPNDIKMKLIENNLTER
                     HARAFLKLPSEDLMQSVLDKVIKNELTVKKTEKLIQDVLEETKVQEEPDKKQNIKGAM
                     SIRIYINTIKQAFDAISNTGIDAKYNEIDKGDYMEVVVKIPKK"
     gene            18864..19637
                     /locus_tag="PC414_000123"
     CDS             18864..19637
                     /locus_tag="PC414_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDF2462543.1"
                     /translation="MGKVIAVFNQKGGVGKTTTNVNLSASLGTLGKKILVLDLDPQGN
                     TTSGYGINKNEVENTIYEIMLDGLHIKEAIISTEFENVDVVPSATELSGAEIELTSKT
                     NREYILKNSIKAVIDEYDYIFLDCPPSLGMLTINCLTAVDSVLIPIQCEYYALEGVSQ
                     LMETIKLVKSRLNADIEIQGVVLSMFDGRANLSIQVVEEVKKYFKGSVYTTLIPRNVR
                     LAEAPSHGKPVIYYDKRCRGSVAYLELAEEFIDLEEEEW"
     gene            19639..20502
                     /locus_tag="PC414_000124"
     CDS             19639..20502
                     /locus_tag="PC414_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HDF2462544.1"
                     /translation="MENKSKRSNRLGRGLSALIPEIKGETSEKEIVNIDIDKIYPNEV
                     QPRKQFDEEKIKVLSDSIKNYGVLQPIVVKMDENNKYMIIAGERRFRASKLANKNQIP
                     AIIKDIDMKDIMEIALIENLQREDLNSIEEALAYKSLIEHYNVTQEEISEAVGKSRPH
                     ITNTLRLLNLGQDVIEMIDSGRITAGHGKALLRIADKDLQLQIAKKIEEEELSVREVE
                     NIAKKISENKQEEPKKSKPKDVFILDVEDKLRNIFGTKVNISKGKKKGKIEIEYYNDD
                     DLNSIVSMLLE"
     gene            20535..21677
                     /locus_tag="PC414_000125"
     CDS             20535..21677
                     /locus_tag="PC414_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HDF2462545.1"
                     /translation="MIYLDNAATTYPKPERVYNAVLDCMKNYCANPGRAGHKLAMRAA
                     REIYDTRENIAKLFNVSNPMNIVFTSNATDSLNLAIKGVLQEGDHVITTSMEHNSVIR
                     PIKALEKRGIENTVVKCDYEGFLDYEDLEKSIKSNTKLIVTTHASNVCGTLIDIKKVG
                     EIAKKHNILFLVDASQTAGVYDIDVNECNIDMLAMPGHKCLFGPQGTGILYVREGLNL
                     NILKEGGTGSKSEEIVQPELFPDKYESGTHNTPGIAGLNQGILFIFERGINNIRQHEE
                     ELCQYMIDKLEEVPDIKIYGPKDSKKRASVIALNIGDMDSGEVTFLLDSDYNIATRSG
                     IHCSPLAHTTLGTLKQGAVRFSIGYFNTKDEIDKAVEALKKISKNK"
     gene            complement(21811..22386)
                     /locus_tag="PC414_000126"
     CDS             complement(21811..22386)
                     /locus_tag="PC414_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HDF2462546.1"
                     /translation="MKNKKIMLVLSILSISIFAVGCTNAQNGSDSSKKETKSNTNVEQ
                     PKEENNTEKEVPNNKAKPTPKEETKTQSATIYSFDVDKTDLIENKVDLNKIDENTLFE
                     ELQKLKVVPESAKLNSFTTKDIDGVKTGILDVSSDFTKSNLGSDAETLMLDSVARTYI
                     KNMNVEQIKITVDGSNYESGHIVLEEGDYLK"
     gene            22646..23938
                     /locus_tag="PC414_000127"
     CDS             22646..23938
                     /locus_tag="PC414_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDF2462547.1"
                     /translation="MEILSLGEKIKKLRKEKNMTLKELAGDRITAAQISHIERDKSHT
                     SYELLEYLGDKLDVSVEYLLETKEMQSKKITDNLILQSEIYIKENELNKAEDQIKEVL
                     DICKRYDLMENYGRCNFLMANINLKKSNYNDAIENFEKALYFFIKNSDSENILKCYLN
                     IGKIYMQEDFYKGAISHFNFAEEILSENKFEDVNIHKELYSKMAYCYIKLDNPNMSLK
                     YIEKINEIDNRNDRKEDVDILVLKANNMLEIGKYEESRDYFKKALKILESEDNKTGLA
                     NVYLTICDVYRKIGETDRVLEYSQKVYDIKKNDEDEYMMSGLFKIIEAYIDKEDYDLA
                     RKYCKIALASSIKNKNKFNEYKALRFYANMYKNQNEVTLAIEYLIKCIKIVSDLGNNR
                     ILANLYIDLGQLYSNISKEKELEYYQKGVFMYKNLEIM"
     gene            24043..24645
                     /gene="yedF"
                     /locus_tag="PC414_000128"
     CDS             24043..24645
                     /gene="yedF"
                     /locus_tag="PC414_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HDF2462548.1"
                     /translation="MSIKIDARGLACPKPVINTKRELDNLEEGVVVTIVDNETAKENI
                     LKLAKSLSCEANIVDEKENFISIEIRKGKNILDKQNVVDNKESELDNTCIFISSDKMG
                     LGNDELGKVLIKGFIYTLTESKPYPKYVLLVNGGVKLSAENEETIENLKILENMGVEV
                     LSCGTCLDYYNLKDKLQVGTVTNMYTIVETLKNASNTISI"
     gene            24718..24981
                     /locus_tag="PC414_000129"
     CDS             24718..24981
                     /locus_tag="PC414_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HDF2462549.1"
                     /translation="MNQMYIVSFNSTHHAIRSEKLFGENSLKVMALPTPREITASCGI
                     SIKFSFEDMEKIKTILVENNVDIKGIYCISKLENGSKEVEKLD"
     gene            24996..25181
                     /locus_tag="PC414_000130"
     CDS             24996..25181
                     /locus_tag="PC414_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HDF2462550.1"
                     /translation="MPMDLKIGDIVELKKQHACGCKEFEIIRTGMDIKIKCTKCSRLI
                     MLDRETLEKRVKKLIKK"
     gene            25284..26480
                     /locus_tag="PC414_000131"
     CDS             25284..26480
                     /locus_tag="PC414_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF2462551.1"
                     /translation="MAVKYAKRMQGLQGSEIRELLKLTQQPQIISFAGGMPAPELFPV
                     EEMKKVSVAVLEENGRSAMQYTTTEGYEPLREKIAARMNDKNKTNVNKDDILVTSGSQ
                     QGLDFAGKVFIDEGDVILCESPSYIGAINAFKSYQPKFIDVPTDSDGMIMEELEKILE
                     TTDRIKMIYVIPDFQNPTGRTWPLERRKKFMEIVNKFEIPVIEDNPYGDLRFEGETLP
                     SLKSMDTKGLVIFLGTFSKIFCPGYRLGWTCASPEILSKFNFAKQGADLQASTISQME
                     VSKFMDMYDLDAHVDKIKAVYVKRRDVMLKTMEEEFPEGLVFTHPEGGLFTWVELPSN
                     LNAKELMPKCLDKNVAYVAGGGFFPNGGRENTFRLNYSNMPEEKIIEGIKNIAAVLKE
                     AMGVEA"
     gene            26694..26972
                     /locus_tag="PC414_000132"
     CDS             26694..26972
                     /locus_tag="PC414_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HDF2462552.1"
                     /translation="MRNYELVYVVKPNSDEEVREAILNKVKEVVATDGEIVKVDTWGT
                     KKLAYPIAKFTEGFYVLVNFKSAVDVPKEIDRNLKINENVIRHMIVVA"
     gene            27009..27443
                     /gene="ssb"
                     /locus_tag="PC414_000133"
     CDS             27009..27443
                     /gene="ssb"
                     /locus_tag="PC414_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDF2462553.1"
                     /translation="MNQVVLVGRLTRDPELRYIPGTGTPVASFTIAIDRDYVKKDGSK
                     ETDFIPVEVMGKSAEFCANYITKGRLVALQGSIRVDNYQTQSGEKRTFTKVSTRSVQA
                     LDSKNKSENSYRESAPAFEPSFEPQGLDPQGFQAIDDDDIPF"
     gene            27462..27689
                     /locus_tag="PC414_000134"
     CDS             27462..27689
                     /locus_tag="PC414_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HDF2462554.1"
                     /translation="MMNKKRRKKKRVCQFCADKNAKIDYKSTQRLQKYITERGKILPR
                     RISGTCAKHQRELTVAIKRARNIALLPYTLD"
     gene            27832..28152
                     /locus_tag="PC414_000135"
     CDS             27832..28152
                     /locus_tag="PC414_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HDF2462555.1"
                     /translation="MKIDKGSEITRNIKIIEWMKTEILMSVSDLFNLLFKGVKPLDEA
                     IQDTLANIIMITYLLAKRLGISFRDVDYKVKEKIKIGIDEDHSVERWYGDLSNLKKHM
                     DNRE"
     gene            28160..29116
                     /locus_tag="PC414_000136"
     CDS             28160..29116
                     /locus_tag="PC414_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HDF2462556.1"
                     /translation="MNNKIRLSKAMSIIVLAIIIALVIAYIPMLGIFSMLAAVPYVII
                     GAITDRKYSLISILVTFCVLILFADISYALNICIMYSLPGIVIGKMLKRSFEQEDSNK
                     FEPIYGGTIIFVLSMLVYFFVLKTFLNVNLLDEVSKMISEIVNIQKNSFSATELKVFD
                     KMKPDEIVSYFTNMLPMMLFLQGLLSAFVTYYLSVFFIKRITKMNIRFPKFADFYLPG
                     NAILTTFLLYLLVLFIQIIGSKLYTELIMTNLQLVFNLMFVIQGIAVCIYFLKKWIRQ
                     GPNKIMFFSGIILCLFGFMGISFVGMVDSIIDFRKVRSYKST"
     gene            29127..31121
                     /locus_tag="PC414_000137"
     CDS             29127..31121
                     /locus_tag="PC414_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HDF2462557.1"
                     /translation="MSNKQTFKLNMPEINLYIIVIGISSIILLYYNLYVGCLFFCIFV
                     YMVFHNWRTTNIRRHEWTEYIQNLSLDIDETTKKAIINLPIPLCILEFDGNISWYNGK
                     FYDMIGQKDLLDKNIEDIVKNLNLRKVLNENKEMYTEINYKEKEYTIIYNVIKNDQEK
                     NPKYLMILYWIDKTEYLKVKQNYDDEKNAMMLIQVDGYDEVLKSAAEDKRALINVEVE
                     KILSALELNSNGALRRTSKDKFFLVMHKKELKKLEAEKFSILDTIRHIDYGNNLPVTI
                     SIGIGIDGDTLNENLKLATGALDLALGRGGDQAVVKTKDKFVFYGGKSKAVEKKTKVK
                     SRLIGHALREVIQQSDQVYIMGHKYPDMDAMGAAVGVYDICKSCNKTANIVLQSVNES
                     IEIFINKINENNYYKKLFIGKEEAIDNCTKNTLVVVVDTHRPNYTECEELLKLSEKVV
                     VIDHHRRGVEFINDAVLLFHEIYVSSTCEMVTELVQYMDEDVTINKLTAEGLLAGISL
                     DTKNFAFKTGVRTFEAASYLRKVGADTIEVKKFFNSDVKDFIIKAEIIQSTKIINNRI
                     CLAYSSTEIDSINVIIAQTADELLNIKEVEASFVLGEKDDTIFISARSLGQINVHVLM
                     EKLGGGGHIDIAGAQLKNVSLKEAYKMVNKIIEEYLEEEE"
     gene            31122..31571
                     /locus_tag="PC414_000138"
     CDS             31122..31571
                     /locus_tag="PC414_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HDF2462558.1"
                     /translation="MKVILLKDVKGTGKKGEMKEVSDGYARNFLFPKKMAVQADSVAI
                     KELNEKNKSKEIKAQKEYEEAVLLGKQMEEINIEIYSKAGEGGRLFGSITSKEIAEQL
                     KKQKDIDVDKRKILLDEPIRSLGSTFVEIKIHQKVTTKIRVDVKEKQ"
     gene            31590..32918
                     /gene="dnaB"
                     /locus_tag="PC414_000139"
     CDS             31590..32918
                     /gene="dnaB"
                     /locus_tag="PC414_000139"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDF2462559.1"
                     /translation="MEDMTRIPPHSVESEQSILGSILLDKDAIITVTETIKPDDFYKE
                     AHKIIYECMITLSNKGEPIDLITLTEELRKQGHLNDIGGISYITSLSTIVPTTSNVKY
                     YADIVKEKSVLRKLIKASNEIINLGYSGATKIEDVLEQAEKSIFDISQEKTSDDFKSI
                     NLVLMDAYDMIEKLYTNKSDVTGITTGFKDLNKKINGLQRTDLILIAARPAMGKTAFS
                     LNLVQNAALKGDASVAVFSLEMSKEQLVQRMLSSQSSVELKKIKTGTLNDNDWPRIID
                     AMAVLSDAKIHIDDTPGIKISELRSKCRKLKIEKGLDLVLIDYLQLMEGEGNNESRQQ
                     EISKISRSLKILAKELNCPVVALSQLSRAPEQRADHRPMLSDLRESGAIEQDADIVMF
                     LYRDEYYHADSESKNIGEVIIAKNRHGETGSVELVWLGEVQRFGDKLRDL"
     gene            33123..33431
                     /locus_tag="PC414_000140"
     CDS             33123..33431
                     /locus_tag="PC414_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2462560.1"
                     /translation="MEKISLKYIYPNIIKVLDEINLFRVIDNNLRESIVVYANNVDNQ
                     YHINMTNTNFGNIINICKLEKLLDVDKFMEKVIKYEKEIIEKEEFSKIEEYMLNIGEY
                     "
     gene            33513..34802
                     /locus_tag="PC414_000141"
     CDS             33513..34802
                     /locus_tag="PC414_000141"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HDF2462561.1"
                     /translation="MKTVAIVGSQWGDEGKGKVIDYLATQADVVVRGQGGNNAGHTLV
                     VEGKKYALHLIPSGVLNPNTVNIIGNGIVFDPKGFLEELEMFKTDNISTENIKISDRA
                     HVIFPYHKELDALSEEARGDLKIGTTKKGIGPCYMDKTERSGIRICDLMDKDKFAIKL
                     KAQIDAKNEIVKNIYGKEELFDFETIYNEYLGYAEQIRKYVADTSVIVYDAVRAGKKV
                     LFEGAQGTLLDLDLGTYPFVTSSHPTSGGFAIGAGIGPNMIKDVVGIVKAYTTRVGEG
                     PFVTEQINETGDKIREQGHEFGVTTGRPRRCGWFDAVIVKYAARVNGLTSISFMLLDV
                     LTGFDKIKVCTSYKMGDKIITDFPASLDDLAKCEPVYEELDGWNEDITQIDNFDDLPE
                     NAKKYVAKIEELVGVSVDMVSVGPNRAQTIIRRNIFA"
     gene            35128..35203
                     /locus_tag="PC414_000142"
     tRNA            35128..35203
                     /locus_tag="PC414_000142"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35161..35163,aa:Lys,seq:ttt)
     gene            35210..35284
                     /locus_tag="PC414_000143"
     tRNA            35210..35284
                     /locus_tag="PC414_000143"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35243..35245,aa:Glu,seq:ttc)
     gene            35289..35364
                     /locus_tag="PC414_000144"
     tRNA            35289..35364
                     /locus_tag="PC414_000144"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35322..35324,aa:Val,seq:tac)
     gene            35398..35472
                     /locus_tag="PC414_000145"
     tRNA            35398..35472
                     /locus_tag="PC414_000145"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35430..35432,aa:Thr,seq:tgt)
     gene            complement(35855..36847)
                     /locus_tag="PC414_000146"
     CDS             complement(35855..36847)
                     /locus_tag="PC414_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2462562.1"
                     /translation="MNEDKIRKILAKYAKRRDDNELLLEHRKNEVYNRIPEIKSIDDE
                     ISKIGLSLAKIVLLNPKSKDEIVKKTKENIESLKVKKERLLAESNIPLDYLEIKFQCI
                     SCKDKGFLPNGEKCSCLKQEIVNEAYKMSNLDRILSQENFSNFNLNIFSPKKGSDGEI
                     SPRENMLNNLSICENFVHDFKKDNSENLLFYGSTGLGKTYMCNCIAKELLDKGNVVIY
                     QTSFRILDILEDYKFRRDTNNQISEDNYKNLFDCDLLIIDDLGTELNNSFTSGEIFNI
                     VNTRLVAGKKIIISTNLTPSQIGNTYTQRTLSRILDKFRILEFTGDDLRWERFK"
     gene            complement(36856..37914)
                     /locus_tag="PC414_000147"
     CDS             complement(36856..37914)
                     /locus_tag="PC414_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HDF2462563.1"
                     /translation="MFFKESNEIDLGEITIPNIFIDIFMPMADGLYVKVYLLGYRQAC
                     DITSNPKFDNNSIAKNLNIPLSDVLSAWKFWEEKKIIKIHDNEEYDNFNYSIEFLDLK
                     NFYIENILSNNSSIKSNTDKVVSTSENPSIRKMFNSINKIVGRYLDPSEKMSIMDIMN
                     KYNMSPDMILCAYEYVKDKTGTSKPVKYIEGIIRNWYDSNLYTPKDVEESFLVRSERY
                     ILYKTIFNELGFSRQPSKSEKELMDTWFDKFNMDIDLIINACSKSKNISNPSISYING
                     IIKNWNEKNIKNLNDLKQKEEERIVKENINKKQINTTQNNNTYKKTKFHNFNETFTQY
                     TSDELDEIIKKSQKEKFK"
     gene            38039..39502
                     /locus_tag="PC414_000148"
     CDS             38039..39502
                     /locus_tag="PC414_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HDF2462564.1"
                     /translation="MVIKFNQKVRRTLSVAILFMVLIIGCFIEKDDSLETYKEKTNSK
                     INQYNMDVIFDDETKRLMCNQNVDYINNTKSNIDKIYFHIYPNAFSKKDFAPFEKSEM
                     TRAYPNGFNEGYIDIKNILNNNSKMEYKIKGDKNDILEIELGKELKPNERMSLDIKYN
                     VKIPNSIGRFGYGENTINVTNWFPIACVNDERGWNLKSYETIGDPFYSETSDFHVKLL
                     IPNKYKIAHTGKLIDDKHDNKKMLYEIEAENVRDFAFILSSKFDVNKVDSKGVAINTY
                     NLNKKLSKKATEVAKDSINIFSELFGKYPYKEFSVVASDFFIGGMEYPMLVMIDQSLY
                     NEKNEFLLEYVIAHETAHQWWYSAVGNDEISEPWLDEALTEYSTIVYFEQKYGKDMAN
                     KLIKTMEIQTKSYLSENIFKPANEYKNSTEYSLNVYTKGAIAFNEIRKEVGDKVFFET
                     LNEYYNKYKHKNANGRAFVELWNSKGVDINKIISECK"
     gene            39604..40401
                     /locus_tag="PC414_000149"
     CDS             39604..40401
                     /locus_tag="PC414_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2462565.1"
                     /translation="MLKYCSVGSGSSGNCHYIGYKNTNILVDAGLSGKRITTGLKDIG
                     VDADKLKGIFITHEHVDHIKGAGILSRKFDIPVFANIKTWCSMKDKLGDIKDKNMKVF
                     ENDKTYSLGDIIVRPFSIEHDASDPVGYNFYTESDEKMSIATDIGCITQNIKKHLYKS
                     KLVVLESNYDPNMLMMGSYTYALKKRVMSSTGHLSNEDAANFCVELINEGTESILLAH
                     LSKENNFPELAYETSKGVLASNDIVVGQDVKLDVLSRNDVSNVYEMK"
     gene            40414..42516
                     /locus_tag="PC414_000150"
     CDS             40414..42516
                     /locus_tag="PC414_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC/EAL
                     domain-containing protein"
                     /protein_id="HDF2462566.1"
                     /translation="MLKRNFENVEDNKILYSIKQGMILAIPAIMTGSTALVILNLPIK
                     AYQEYLSGLFNGEVTNILNFINDSTLGIISLIILLTISYSYGKIYGSKYTVLVPIVAM
                     CSFLVFSHGNELNSYIEIFKTKWLFTSIIVSMTSSVLFVKLTESFVTSVKFHTEGADA
                     DFNMVVSAIVPFIIVVFLFSIFRVVMISLIGSSNFQDIFYNLFSNVFNKMGTNLMSAL
                     LFIFLMHFMWFFGIHGSNVLDTVAKNLFENSMAININLVNNNQLPTEIFTKTFFDTMV
                     LLGGCGSLLCLVIAIFLSEKRINVRKLAKIASIPALFNINEMLVFGIPIVFNYIMFVP
                     FVITPIILTVTSYVAMSTGIVPCTVSAVEWTSPIFLSGYMSTGSIRGSLLQVFNLCVG
                     VMIYIPFIKMSQNRYTYMFKNNIENLTNLLKRYELTGEQPNLLNHNGKIGSISKMLAS
                     DLKFAMKRGEIELFYQPQVNYNGNVVGAEGLLRWKHSIGGFIYPPLVIILAKEEGFLD
                     ELGEYIINKACIDLKKSEKLLRNPVKFSVNISPDQLDDPKLPEQIKNIISSNDINPNM
                     LGIEITEQIALSGSQIIIERINAIHNLGIKLIMDDFGMGHSSLIYLQNNNFDIVKLDG
                     SLVKEILTNKRSSEIIMTIVNLSKNLDFDIVVEYVENIDQRDKLYGLGCEIYQGYYYS
                     KAIPFEEFVEYANQEREI"
     gene            42584..43063
                     /gene="rlmH"
                     /locus_tag="PC414_000151"
     CDS             42584..43063
                     /gene="rlmH"
                     /locus_tag="PC414_000151"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HDF2462567.1"
                     /translation="MNISIVVVGKIKEKYLKLGIDEFKKRLSKYCKLEIIELDDEKAP
                     ENLSVKEMEIIKDKEGKKILGKIKHNSYVIALAIDGKGLSSEELAKTMSDLAVRGNSS
                     LCFIIGGSLGLSDEVLGRADYRLSFSKMTFPHQMMRLILLEQIYRAYRINNGEPYHK"
     gene            43212..43523
                     /locus_tag="PC414_000152"
     CDS             43212..43523
                     /locus_tag="PC414_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2462568.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMESAAKENGIESQIWAIPESTIQN
                     EIEKCDVLLLGPQVRYVLPKAQEIAKPYNIPVEVINMMHYGTVNGEAVLNRAIELNNT
                     K"
     gene            43571..43888
                     /locus_tag="PC414_000153"
     CDS             43571..43888
                     /locus_tag="PC414_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF2462569.1"
                     /translation="MDEKTIEISFNIIGYAGEGKGLAFEAIKEAKNGNIEKAKELLKE
                     SREVINKAHRYQTELIQNEASGNRTEISVILIHAQDHLMNGMNFQQLAEEIVDLHLKL
                     QDK"
     gene            44101..44802
                     /locus_tag="PC414_000154"
     CDS             44101..44802
                     /locus_tag="PC414_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2462570.1"
                     /translation="MLRNQPLYIQVVDILKQKILNNEYKIDENIPTERELEQEFGVSK
                     ITIRKAIEILESEGYVEKKSGKGTKVISNSLFNKLSKAESFSSILLSKGHKLSKETIS
                     IEKIINDTNSQVFSIFGEKCSKITRMYYLDSKPYIFFTHYIPFDENLTVESFNNESSL
                     YMYLYRRNINITRFEDEFFIEESELEVSKALNLAKDILLGRKRKTYDENDNIVELSFA
                     RYNTEVQNYVIKYEI"
     gene            45026..46327
                     /locus_tag="PC414_000155"
     CDS             45026..46327
                     /locus_tag="PC414_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2462571.1"
                     /translation="MEKFASFLERKIMPVAAKISNQRHMRAVRAGIIATLPLTIVGSF
                     FTILLNIPIDAYMDFIAPFKAILDIPFRYTVGFLSLYATFGIASSLAKSYDLDTTGVG
                     MLAVMAFLVSTIVPTQVVEPVGGVIEAGRWMDMAKLNSPSLFGAIVTSLITVEIYRFM
                     KEKNITIKMPAGVPVEVSNSFTALLPTAVILMLFWVIRHILGFDISVALSAMLSPLKG
                     FLSGNSLGGGLLTVFLITLFWVLGIHGPAIMGPVIRPIWDMSIAENITAFTEGVSAHA
                     MPNIFTEQFLQWYVWIGGAGATLALVVMFMFSKSQYLKSLGRLGFLPGLFNINEPIIF
                     GAPIVMNPILGIPFIIGPIIMTILSYVLTITGVIPMMVARLPFAMPAPIAAVMSTNWS
                     ILAGILVLINFVISFAVYYPFFKVFEKQQLQREQEEMSENL"
     gene            46464..47558
                     /locus_tag="PC414_000156"
     CDS             46464..47558
                     /locus_tag="PC414_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDF2462572.1"
                     /translation="MERRLGISVYPEHSNMEDDKAYIKMASKYGFKRIFMCMLSITRP
                     KEEVKKHFKEIITYARELGFEVILDIAPNIFDILQISYDDLTFFSELNASGIRLDYGY
                     DGSKEAMISFNPYDLKVELNMSNDVAYLDNIITYKPNVNNIYGCHNFYPQRGTGLPYD
                     FFVRCSNRFKKYGIRTAAFVNSKYATIGPWNINDGLCTLEMHRNLDIATATKHLFATG
                     LIDDVIVGNAYASEDELKRMSEVNKYQVVFNVVTEEKTSEVEKTIMANEQHFRRGDIT
                     NQVARSTEVRKKYAKENFPKHDNTTIFKRGDVVIGNDDFGKYKGELQLILQDCADERK
                     NKVGRIKEDEIILLDFIEPWSKFKLILEGE"
     gene            47560..48693
                     /locus_tag="PC414_000157"
     CDS             47560..48693
                     /locus_tag="PC414_000157"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HDF2462573.1"
                     /translation="MKIDILIKCGKTVDKSIIDIAILDDKIIEVKNQIDDSKYQANQI
                     INLDGEKFISAGWIDIHTHCYERLDLYKDYPDEVGIKSGVTTVVDAGTTGALDIGKFY
                     EDTKKYKTNVYALINIAKQGITSQDELSSMMNIDEYELRRAVKKYKDFVVGIKARMSK
                     SVVISNDVEPLKVAKRIKNELNLPMMVHFGSSPPTIEDIFDYMEKGDILTHIYNGKPN
                     GILRGNEVKKEIIEARERGIILDVGHGTESFSMDIAMKSKDAGIFPDTISTDIYIKNR
                     INGPVYNLSTTMEKFIYMGYSLEDIIDKVTKNAADAISLKNKGLIKEGYDADLTIFDV
                     VNEEKELQDSLNKSVITSTSIKPRAVVVNGEYLNIGKSIGENE"
     gene            48711..49820
                     /locus_tag="PC414_000158"
     CDS             48711..49820
                     /locus_tag="PC414_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HDF2462574.1"
                     /translation="MSSIFEKYSVRRVINASGKMTILGVSTVDKEVIEAIGEGCKNFF
                     IIEELINKTGEYIANLLGCESALIVSSASAGIAQSVGAMIAKDDMSLVYNVNNPKKQV
                     KREIIIPKGHNVDYGVPVEVMIRLGGGEVVEAGYANMCNKEHIIREINENTAAIMYIK
                     SHHCVQKSMLSVLDAVQVAKEYNLPLIVDAAAEEDLNVYYELGADLVIYSGAKAIEGP
                     SSGLVIGKKKYIDNVKLQSKGIGRAMKIGKENIVGLTCAIERYISNDKVTLKEMEDKL
                     NPFIDKINTIKGVSASITRDSAGREILRGEIDFNEDIINKSTQQIINELRSGEIAIYT
                     RDYKANEGKIEIDIRSVTGSDLDTIYNRIKNIVES"
     gene            49885..50625
                     /locus_tag="PC414_000159"
     CDS             49885..50625
                     /locus_tag="PC414_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HDF2462575.1"
                     /translation="MNCIPQYYKNRVCLNVLAGSIKNAKEVYDACDGHVLIGILSKNY
                     STVEAAIEDMKKYSKEIDNAISVGLGAGDPNQSNMVSQISKELQPQHINQVFTGVATS
                     RALLGQNDSIINGLVSPTGKPGFVKISTGPLSSKEKDGIVPIETAIAMMKDMGGSSIK
                     FFPMGGLKTKDEYIEVAKACAKHNFYLEPTGGIDLDNFKEIVQIALDAGVEKVIPHVY
                     TSIIDKETGETKVEDIGVLYKIMKELLG"
     gene            50630..51622
                     /locus_tag="PC414_000160"
     CDS             50630..51622
                     /locus_tag="PC414_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDF2462576.1"
                     /translation="MNILAFGEIMMRLSVPDYKFLTQTNELNYITTGTGLNILSGLKN
                     FGYNTYMLTKLPNNNVGKASSANIRKLGVKDDFITYGGNHIGVYFLENGYGERPSEVT
                     YLNRLNSSFCESKLQDYDIDKCLEEMDIVHICGIAMQLTDSVKEIALELARKSYEKGI
                     KVFFDFNFRQSLNPERTYDDLVNDYKKILPYCHGLFASYRDINKILNIDSLNIKSSSE
                     LIKKEYDIDIVAGTMRRFDDMNNRFIKGYISNDDGYFESREYKLEIYDRVGAGDGYVA
                     GIIYSYLENMKSEDIVEFGVASSVLAHTTYGDNSLVSTGHVLRLMNNEKIDILR"
     gene            complement(51810..52691)
                     /locus_tag="PC414_000161"
     CDS             complement(51810..52691)
                     /locus_tag="PC414_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2462577.1"
                     /translation="MNTKQLEYFISVAENLSFTKTAEKFYISQTAVTQQIKALEEQIN
                     VTLFTRSKRHVELTPAGKVFLSEARTIIKNINDAIAKTQQFAHGFFGTLSIGTLIGYE
                     KNKLQQYLKEFSNTYPNISMDICTNEITELLNLVKNNSMDLAFVINPENQPLKDFEYK
                     TVERYSLVALLPPGHPLYNEDSIDLIELQHDKFIFVKETGDEYGQKSMIQNRYREAGF
                     VPNVVQRSNNLNTIESLVSSNMGISILPSFCVTDTMIERDIAIVPIKEVENRIEVVVV
                     WNKKNTNPALEKFISII"
     gene            52964..54352
                     /locus_tag="PC414_000162"
     CDS             52964..54352
                     /locus_tag="PC414_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2462578.1"
                     /translation="MEDKFYAKGNGNNGYIKNLEVCSFNNLDGTCGMFQMALYKRDEK
                     YYLYGCCFGGNKKNGVMISDITDPYNPQFIKHFQMLDPKEYPTTTTPKIQIADDLMIV
                     AMSCGSGPGALVDQAKLANIKCEAGIRIYSLKEDPLNPKFLGYWDCGLKHVMGVHRFM
                     YNGGRYVHLSSDCVGFEGLIYRVIDIINPTNPVEIGKWWRPDQYANGYPNRTFDAGAP
                     HCPEFMDKGWLHGPPFVRDGKAYCGYGGAGLVVLDVEDLTRPRCLGELPFMPAFSSRL
                     AGARTHTALPLPGRDLVVVQNEGERFQFFKPDNITDVQAMNNIHMVDVSDPTKPTLIA
                     QFPYPEVPKDFPYPNFNVAGLGKPGPFGPHNLHEPMDNKPWLEQRGDRVYCCYFHAGL
                     RVYDVSDPYYIKELAYFIPPNPNKTPEESYFPGFPGPRLAVTEDLIVDDRGYIIIDAL
                     DDGFYILKMKED"
     gene            54380..55333
                     /locus_tag="PC414_000163"
     CDS             54380..55333
                     /locus_tag="PC414_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDF2462579.1"
                     /translation="MKILGISFGTKNGNNDTMCKVALKGAKEAGAEVEFIQMSSINIK
                     HCTGCCACVKTLLSGKGSMCVLKDDFEWLLDKMKDADGIVVSDPIFEEGASGLFHTIM
                     DRFGPRADRGNNIIGTKVAEAIGGKIPDSRMLNDKVISFMGIGGSDWGTRVQCEHAML
                     ALIPMWKVIDNAWFPWAKELVMDDTRLVQVHQIGLNIVEAAKDFEKASYQGEEGVCPH
                     CHNRLFYLEPGTKKAICALCGIVGELDTISGKTIFSFPEEQLGHAHDTLPGKFIHGDD
                     IKKMEGHYAEVRKTQEFKERKTSYNFIEPLTKEKQAGVSLG"
     gene            55393..55617
                     /locus_tag="PC414_000164"
     CDS             55393..55617
                     /locus_tag="PC414_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2462580.1"
                     /translation="MNEKSSFKQIEIILAVIFLVLTYYKVRLTPAFIVIFIPLYLSYF
                     AVSMIVNQIAIKLLEIRQAREATNYNIGNG"
     gene            55610..56122
                     /locus_tag="PC414_000165"
     CDS             55610..56122
                     /locus_tag="PC414_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HDF2462581.1"
                     /translation="MDRKKKEILTLSMGIALLPPLWAVLAPYIGIKTGAVALICAGLY
                     VTNGNKQKDGLKIMFGFWCGDLWAVLAILIMGYMNFNQNLELFLTLSILGFFAVVIAS
                     LFEKIIFLPSWLCGWAIGLTIMTGENIINLQDICIQIAVAMAVGVWYVGAGVDLFTIY
                     ILSKDRKKGN"
     gene            56154..57008
                     /locus_tag="PC414_000166"
     CDS             56154..57008
                     /locus_tag="PC414_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase"
                     /protein_id="HDF2462582.1"
                     /translation="MAMGFEFKIMRSLIYVGLAKEEYRPKLMDWLYRHHIPDSISTFG
                     PYCTKYAFYQAYPTPNEGERFGARKMQLTEHYWLVDEHMPEMANRIMTEYMPMDVLRW
                     QGCIPDVENKRVHENAESGDAGRAVGGDNGCPPFIFAFVPINWEEDFRGKGRTVQDGP
                     NYRWQFMIKYPDGISKEEGEKWFYDEVVPYFTNCCYVNRFVSSKIMINYGATAFDRVS
                     ELWFEGEEEWYKAVVEETKSFIKKPEWAQEEEFPYLKPQFNIASVFLGDIATMDAYSQ
                     YRGYIPMR"
     gene            57542..58336
                     /locus_tag="PC414_000167"
     CDS             57542..58336
                     /locus_tag="PC414_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2462583.1"
                     /translation="MYTIGQVSKFLGISRDTLKFYEDKGLVNPKKNDENGYRIYNQVD
                     IYDIATINFYREIDIEIKKIQEIRKSKSINNLELLLEEKEQIILKEIEYKKLLLKKVQ
                     LVKEDCDKIKTYFGKYTIKEMKPLEVKGKIKHVTNYEEYEALKENTNNLKKAVTLTDL
                     RRVINFNEEGIIEEKFIIVREVDDYNKIIEGEVLSHPNCIYTIIEDGRWSTGGENIDN
                     KVEDRLREIAIENECELLGIVYVNILLTTYEEGIERIFLEIYAPIK"
     gene            58440..59246
                     /locus_tag="PC414_000168"
     CDS             58440..59246
                     /locus_tag="PC414_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF2462584.1"
                     /translation="MRTEKSLKRPIASFIILTNIIFLPLFLLVVVTIMLKLPTVISDI
                     ALCISAWSSTFAFMILFKKIYPGKKFLVHVKDRFRNKLKFSTVSIIISIQVLIAVVVI
                     FILASKNHGIMPNFTVSSWGAFIYLFLKNLFAGSLGEELGWRGFVQNHLQKRHSPLKA
                     SIIVGFWWGMWHLPIWFTTGFKGLDLIKYIIFFMIAIISISIIMTTFYNLNKTLIIPI
                     VIHQLFNFLIGIINGDLIELINYYAMLYLIFAIALIVINPGNVLYKNIRE"
     gene            59308..59829
                     /locus_tag="PC414_000169"
     CDS             59308..59829
                     /locus_tag="PC414_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDF2462585.1"
                     /translation="MVLLIVDAQKLITNERLYKFNEFVVNVENLIDTARKNNIEVIYV
                     RHDDGVENELTKGKNGFEIYEKFKPCNGEKIFDKKVNSAFKETGLLEYLTNNGEKDII
                     IAGLQTDYCIDATIKCGFEHGFNIIVPAYSNTTVDNFFMSAEQSYKYYNEFIWNERYA
                     KCISLEETLRKMK"
     gene            60244..62331
                     /locus_tag="PC414_000170"
     CDS             60244..62331
                     /locus_tag="PC414_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2462586.1"
                     /translation="MVVDKEIVSILQILLNSTLITTNGLQEESNSSKRQITYRINKIN
                     DMLKVKNVPLICLRADKDIIVRKETKKAIKEILEKNYSKNTYYFSKTERLLYMYLMLF
                     INMDDISINHFINSIKVSRSTVNLDFKDLIPELEKKNIKVKNNRINGYYIVGNEMEIR
                     RVLIKNIIETLSNEKSSRVFDIFIKEYNLDSFEEAKKIILKLIKKYEITFVEDRLVEF
                     IYIFIFLKARMYSTRIVTQENVDIPNISVMSSMKEYKFAKELLEVYGSEDKIKPHNIM
                     YISAWILGISVGNYEEDTEDKDVISKIVNKMMTKFGYLSGVYYISQEKIFKQLYSHFR
                     PAYYRLLFKLPIYNPICEKVKEEYKLVYRIVSNAMKEFCGLFGEEIQEEELAYLTMHF
                     ATLFSNKKEFDGFRKRIALIVCSNGVGSSAILHAELTNLFPDLHFIPMSESVGLENIS
                     EPVDIVFTTNYNAEGLKTDVPVIKVSPVMTTKEKYKITREVYIQLGDIFLRQPKIDEV
                     MDIIKKYANVTSESILSSELLAYFTQIENFTSKEGEGPMLSEITNETLVKIKVKAKNW
                     EEAIRQSASVLVENNKVTEGYVDAMINSAKASGPYIVITKHVALPHARPEAGSKEIAI
                     GIATLDSPIEFGNNENDPVKYVFCLSAIDNNSHLRAMSELVELLEEDEFFKVLDSAKE
                     AKEIIDFIKTHEL"
     gene            62346..62633
                     /locus_tag="PC414_000171"
     CDS             62346..62633
                     /locus_tag="PC414_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2462587.1"
                     /translation="MYKILIACRAGVGSSLMLKIKTQQIIKENNFPIEVEHGSLDSLN
                     GFNGDAVETLIDVAEELKNKNLKFDIIGIHNIVDRNEIKTALEKFLASKEG"
     gene            62660..63931
                     /locus_tag="PC414_000172"
     CDS             62660..63931
                     /locus_tag="PC414_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HDF2462588.1"
                     /translation="MEFIISLLSTPAVLLGVVACIGLILQKKSAVEVFTGSSKTLIGF
                     LIFGIGASAMTGALQNFNKLFQHGFGITGVIASPEAATALAQGTYGFAVSCTLILGFI
                     LNLVFARITKFKNIFFTTGHSLFFSCVLVLIMKAHGFDNTITILIGGTILGFMSAALP
                     QFCQPFMRELTGGDEQAIGHFNMIGYGLSGYIGRLFSKHKDDTTETIEFPKWLSIFRD
                     FLMGLSIVMLILFYIATLKAGQAFTQEIAGSTHWLVFPLIEAFTFVAGMSILMSGVRM
                     FLAEITAAFVIISEKYIPGSRPALDVPTVFPYAPNAVIIGFISAYAAGLLAIAIMASF
                     PSIFPVVIIPAAHICFFSGGTAAIFGNTSGGWRGAIAGSFVVGLLLAFLPVILYPVYG
                     TLGIEGATFPNIDYNVVGSILHNILQFIKPI"
     gene            63989..64588
                     /locus_tag="PC414_000173"
     CDS             63989..64588
                     /locus_tag="PC414_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDF2462589.1"
                     /translation="MENTLSEFLDNISNELSEFIASIDETSINKACELILEAEKNHGR
                     VHVTGIGKPGHVSGYISSLLSSTGTSAYILHGTEAVHGSSGQVVEGDVVIAISNSGET
                     QELKATLKTLKVNGAKIIGVSGNESSFLKNISDIFLFAGVKQEGDCLNKAPRASILAE
                     TIILQSLSVVLQYAKGLNTQEYLKWHPAGSLGKSIREGI"
     gene            64593..65498
                     /gene="dapA"
                     /locus_tag="PC414_000174"
     CDS             64593..65498
                     /gene="dapA"
                     /locus_tag="PC414_000174"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDF2462590.1"
                     /translation="MKYKGIICAMITPFDENQNINSQATCQLIDYLIEKGIYGLFILG
                     TNGECHVLTDDEKVEFAKIVINHTNNRVPVFVGTGGNSTREVVDLSKRMEAIGASALS
                     IITPYFVTPTQQELILHYKTIANSVNLPIIMYNMPGKTGINIEPESVRELSKIKNIVG
                     IKDSSGKLDNMKAYIEATKNENFSVFSGSDSLILDSLKAGSQGAVAATTNFLTEIGIS
                     IYENFMKGDLEEAQKSQSSIEELRRILKLGTIPSVMKKTIVLNGINVGTARLPVTEPT
                     GEVLEEIKRVVENYRTILNKSSENR"
     gene            65604..66236
                     /locus_tag="PC414_000175"
     CDS             65604..66236
                     /locus_tag="PC414_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketohydroxyglutarate aldolase"
                     /protein_id="HDF2462591.1"
                     /translation="MKKMNITKRMSECGALAIVREENLNRACEIAEGCIKGGITVIEM
                     SYTLNNAGEIIQGLNKKYGEILCVGAGTVLDSETARHAILHGAQFIIAPNYNEGVAKI
                     CNRYQIPYAPGCTSLTEAVDALSLGATFIKAFPISDFYGPKLVKVFKTPIPDMPVLAS
                     GGLNIENLHIWLENGVDVCGFGGLLTKGSVEDIAKNANKIREIIKNVRKI"
     gene            66713..69220
                     /locus_tag="PC414_000176"
     CDS             66713..69220
                     /locus_tag="PC414_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF2462592.1"
                     /translation="MKEDIRLHRMCKKEQATKNEVISEDILKSMSIGVSKHFLDDSFT
                     IIWGNTSFYQLLGYTEEEFLVKFSSLKDYYEQNLYHFESMKNHFLEAYHHGEKSIEYQ
                     ICISANNGDPVWVIMTGILIESQDNEKSMVYILYSKINELKIKQEELSDMEKESTENF
                     KWMMSEYAGNVYISDMDTYELLYLNKHSCNTLQASANELIGRKCYEVIQGRTSPCPFC
                     TNSYLKKDDIYEWEFYNKKLKRTFIIKDRMLNWQGRRVRIELSYDMYSAEYKLAKKDQ
                     ERESILKTIPAGMVRLDARDYLTILWCNDIFLNMIEYTKEQFAEELNNQCGYLFSEDY
                     KRSKKLAQDLKESGDNVVFEAKIYTRSREERIWTVTLCYISGEDSWDGIPSFYSIGID
                     ITKERKQIEKLQHISEKDALTGIYNRAETERQIKKYFEKNLNVMGALFMIDTDNFKQI
                     NDTEGHMIGDIVLTEMASGMKKIMRDSDVVGRIGGDEFTIFMKNISSVKDAEKKAEEL
                     LYMFRHLFQKEKSFLKVTCSIGIAIYPKDGTTFKEIYARADKALYQAKNMGKNNYVIY
                     NQDYFKELEEFDCSSLGTVIDSEKQYAEYPDNLTRYIFRMLYQTDDIDQAINMILEVV
                     GKQFDVSRVYIFENTEDGRYTSNTYEWCNEGISSQMAYLQNSNYQDYEDYEKLFEDEL
                     VFYCRDIHTLSPKLEELFSSQGIHSTLQCAYKEDRVFSGFVGFDECTGLRLWSQEEIS
                     TLSLISQIISMFLQRKKNNKLNQQIQQYQNILDNIDQCICVINKENNFLLYVNKKFKN
                     TYSKFQIGQPYYLDSIDIHKISIIWNKKEAYLCTSTI"
     gene            69562..71724
                     /locus_tag="PC414_000177"
     CDS             69562..71724
                     /locus_tag="PC414_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HDF2462593.1"
                     /translation="MKKIIYVLFTIELLIVSLLGLNIVKDNEINNILYNDTTSISVMF
                     KDYKKLNKNYSKWIKDIADENNVAISKYVFNNNEKLSIYTTDTSLNNNIKLKSGKFPN
                     TNSNEFISNINTNNSNQVGKFSTMSKDISILIRDFGKISEVGESGILYISTQNEDTIN
                     KILRELNVDRSIFVARLHDRYVNSNLYLNPSIIRDLILIILCFLATIIHYSISVSREA
                     SILRLNGYSKLDIIARVIKNILRIMILSSVTALLIYIIYILKLNIGVRACLYFAMFSI
                     ICILINILISILIVGFNSRSSKYVLSLNGKKSYGFVNIMHFTLKIVFVSFLILSINNC
                     ILNYNMLQTQLGNLSEWDKAKNVYNLTLKDTGEQSLGKEEVIKNAKIKGFYKNLVEEK
                     DAFLIDSTNFEKLEDGSYMYEINSKGKNPEVSQYGKNIKINKNYLKVNPIYSNGKEIF
                     NLIDYDDKTINLLVPKKLQVHENEIKKEFRELFYFEKIEVENMYNKELNRELNKTKKS
                     DLNVNIIYVDNNQSYFTYNSLVMDDNRNLIDDPIAIVDIDNIDDSFYLSYISRCVYFN
                     SKKLDALADISSIIEAQGVEAHIQSLHAVYNEYGLEINKLEKYLNSEIFTIIIIAISN
                     LMITYNIVASYYERNKYKLYIKKIFGYSTTARSMLLIVSLILTNIIPIGIISTRVDLS
                     NNIILFGLLILVIEVIVSIVLDKIISNSSFNKIIKGEH"
     gene            71729..72346
                     /locus_tag="PC414_000178"
     CDS             71729..72346
                     /locus_tag="PC414_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2462594.1"
                     /translation="MISLINISKKFKDKSIIDKFNLEISKGEFVAITGNSGSGKTTLL
                     NIMGLLEKPNSGDIIINNIKNPNSKQIRILQRDFYGYLFQNYALIENENVENNLKIAL
                     KYQKNINHNEKINYALESVGLRKYNKKKIYELSGGEQQRVALARVILKECEVIFADEP
                     TGNLDKKNRDIVFDILKSLNKNGKTIVFVTHDLDLSEQADRVIKL"
     gene            72535..72879
                     /locus_tag="PC414_000179"
     CDS             72535..72879
                     /locus_tag="PC414_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HDF2462595.1"
                     /translation="MENLPNCPKCNSEYTYEDGTLLVCPECSHEWALGLGAEEDSNII
                     KDVNGNVLNDGDSVTVIRDLKVKGSSSSIKIGTKVKNIRLIHDSSDGHDIECKIDGFG
                     AMKLKSSVVKKA"
     gene            73449..74315
                     /locus_tag="PC414_000180"
     CDS             73449..74315
                     /locus_tag="PC414_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDF2462596.1"
                     /translation="MIDIHKNIYDNKLFEELKIDCKKCFGLCCVALYFSASDGFPINK
                     ESGKPCINLQPDFKCSVHNSLIKRGFKGCTAYDCFGSGQKVAQVTYKGIDWMQSPELT
                     NQMSEVFLIMRQLHEMLWYLKEASVLNISDTIKSKIDLIIEETEKITNMGPEQIINLD
                     IISHRTKVNLLLSQASESMMGKVKSFIKTSTLKNMKKLSKNIDLIGADLRKINLIGAD
                     LRGRFLIAANLRNTDLSGANLIGADLRDCDIRGANLENSIFLTQLQVNTAKGDSSTKL
                     PASLIRPKYWEK"
     gene            74428..74832
                     /locus_tag="PC414_000181"
     CDS             74428..74832
                     /locus_tag="PC414_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmaF family protein"
                     /protein_id="HDF2462597.1"
                     /translation="MSYQNYKCERDYVYDDYKYNKCNKCYNDYEEMTHVHEYSESVKL
                     AEECEDRHNHRAAGVTGEVIPINGGTNHVHKINDNVDFLDHFHKICVTTGPAIRIPGT
                     DKHIHLICGETTVNDGHCHKFLFTTQIEAPLV"
     gene            74996..75445
                     /locus_tag="PC414_000182"
     CDS             74996..75445
                     /locus_tag="PC414_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2462598.1"
                     /translation="MKSGIILEMKQINSTEYDEMLQVWESSVRATHDFLTEKDIESLI
                     PLVEIGLKEVENIVCIKDNDIIKGFIGIDKDKIEMLFIEDKYRGNGIGKKLIKYAIDK
                     YNVKYVDVNEQNKKAVGFYIHLGFKVFDRSEIDGQGNPFPILHMKLI"
     gene            complement(75601..75978)
                     /locus_tag="PC414_000183"
     CDS             complement(75601..75978)
                     /locus_tag="PC414_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF2462599.1"
                     /translation="MLTPYLIFNGTCEKAFNFYAEAFGGGKTIFARLDSNPNNPIMHA
                     SVTFTKYEGCIMGADTDKPVVISGMAICVVLPSREAIEEISVKLAEGGTLVQEFLPHP
                     PPHQNDGAAEVLDRYGYTWYLST"
     gene            76240..77157
                     /locus_tag="PC414_000184"
     CDS             76240..77157
                     /locus_tag="PC414_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing protein"
                     /protein_id="HDF2462600.1"
                     /translation="MVNMNIIEIRSDKIYKKIMDAPINKKEDIYRYELMKPFEFKWKC
                     MNVPIVARQKGGYDVIIASEMLGVLSPKDIDEKQKKNINVLSADKIWGTCKETIENSI
                     NAFIKEGYDLNIKDYKYSILLANPNSPYTILSDGYWGDGGIPGYIFLSLVPNEYTINR
                     LPVLIAHECNHNIRFQFIEWNNNITLEEMMINEGLAENFATWMFGEEMLGPWVSRTDI
                     ETLNTYIKPIIKSALKETGFQNITSYLYGDDIAKMQGYFPVGLPYCAGYACGYYMIKY
                     YLEKTNKSIIEATLLPYSEIIEAVKEFWE"
     gene            77641..79059
                     /locus_tag="PC414_000185"
     CDS             77641..79059
                     /locus_tag="PC414_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HDF2462601.1"
                     /translation="MTELMIISGLVLLICITSSKVLYKFGVPILLIFILLGMLFGSDG
                     MVGIYFDNYELTKQLCSIGLIFIMFFGGFGTNWNMAKPVAIPSILMSSLGVIITAGVT
                     GMFCHMVIGTSLLEGLLIGAIAASTDAASVFAVLRSQKLNLKGSLASLLEVESGSNDP
                     IAYMLTLILLTLMNNSGVELIAPMVIKQIAFGLAIGFILAKLSVYILRHSNFEIEGFY
                     TIFITAIAILSYAFSEYLGGNGYLSVYISGIIIGNSRIPLKKSLVHFFDGISWIMQIM
                     LFFMLGLLSFPSKLPNVFGIAMAISIFMIVIARPLATFIVLAKFKFSNKEKLFISWVG
                     LRGAASVVFAIYAVTQGVAIENDIFHIIFFIALLSVGIQGSLIPTVARKLDLVDDNTP
                     VLKTFNDYDGEISSKLIEVNVDEGNKWANKSIIDSNIPDDILIVMIKRKEQVLIPKGS
                     TIIKTGDILVLSGDRIEELIQL"
     gene            79135..79947
                     /locus_tag="PC414_000186"
     CDS             79135..79947
                     /locus_tag="PC414_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2462602.1"
                     /translation="MFSIGMFSKINKITTKTLRYYEDIGLLKPEYVDEFTKYRYYTTE
                     QLPKLHQIITLKQMGLSLAEIKKAVENPEEVENILMNKEKEMLDVIKSEEYKIIKLRN
                     YLMNLKGEVNMNDVIIKSLPKVKVASMRKVLKGYDELFNLFPNVMAEEMMKTGCTCAV
                     PDYCFNIYHDGEYRETDMDVEMCQAINDLKEDTDILKFKELDAVENAVCILHNGPYST
                     LGKTYADAFKWIEDNKYKVVGNPRESYIDGIWNKESEEDWLTEVQIPVKRML"
     gene            80370..80987
                     /locus_tag="PC414_000187"
     CDS             80370..80987
                     /locus_tag="PC414_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HDF2462603.1"
                     /translation="MEIFAIGHSNYPYDKLINMIKKYDINCVVDIRETPYSKYNIQYN
                     KEAFNESLRNSGFLYIYMGKEFGAKRTNKDVYTQEGYADFEKVAKEDIFLNGIERLKK
                     GCQMGYRIVLLGAMQEPIRCHRSILVGKVLNKEGFDVKYIMHEGNLAYQEDIEESLLD
                     KYFSDRKQLSIDNLLGSALTREEMIQEGYKLANKEIGYRTEKLGK"
     gene            complement(81144..81596)
                     /locus_tag="PC414_000188"
     CDS             complement(81144..81596)
                     /locus_tag="PC414_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staygreen family protein"
                     /protein_id="HDF2462604.1"
                     /translation="MVNLNANNLNVNMVYPISSTAPIRFRRYTVSYLENMENIYLTIA
                     TYFDTLGLGTMNIDKVYGQWAWFTDNIYDLNLFVFVGNYPYQIAKNRYDTFVDILPVA
                     ITSIVNGDRVFLISNPCLLNSKITVRFISSHPTLNRTVDYGNIRDFIF"
     gene            82044..83402
                     /locus_tag="PC414_000189"
     CDS             82044..83402
                     /locus_tag="PC414_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2462605.1"
                     /translation="MFIRKNVIKLAIPIMIEQTFVMLLGVFNTMMAGHIGEEAVSAVG
                     MVDSINNIFISVFAALSVGATVVVAQQIGKKDSEKVNETIKQALVSGVILSFTITILM
                     WVFRTGVINLFYGSAEELVKSNAKLYIEFTLFTYPFIATQQIANGILRGCGDVKKPMY
                     VTMFMNIVNVTLGYILIYGIDLNPLGINLQTPSYGITGAAISIAIARIIGCIVIIRAL
                     FKGNEFISMGKIFPFKIDVETQKSIFNIGIPAGVEQLLFNAGKLIVQVFIVTMGTSAI
                     ASNAIGMSIASIINVIGNALALSATTLVGQYIGRGDIKGAKSTLLYLTKFATICLFIV
                     GAIFIPIAPWISSMYTQNASVIDISTQLIRSDCLAMVIWPISFVLSAGLKGAGDTRYT
                     MMTAVIGMWIFRIFTGYVLGVVLEFGVLGIWIGMYTDWLVRGTLYCLRLRGTKWLKHK
                     VA"
     gene            83675..84055
                     /locus_tag="PC414_000190"
     CDS             83675..84055
                     /locus_tag="PC414_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF2462606.1"
                     /translation="MINKIGKITLYVNNQEDAKLFWTEKLNFVVTYEKEMGPNMKWVE
                     VAPSKNEFTTFVLYDKNLMMKQNKEINVSHPSIILSTRDINGTYEQMANNEVEVGELM
                     EMPYGRMFSFKDQDGNDYLVREDK"
     gene            84180..84380
                     /locus_tag="PC414_000191"
     CDS             84180..84380
                     /locus_tag="PC414_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2462607.1"
                     /translation="MFKKMAVLKDIATKIGRKKAYELLEMVEGNDAFVAEVKIKKNGI
                     ESKKEEIMLKDNQKIILEYIEG"
     gene            84576..85304
                     /locus_tag="PC414_000192"
     CDS             84576..85304
                     /locus_tag="PC414_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2462608.1"
                     /translation="MLEIKNLSFNVESNNEELGIINDVSLSFERGKLIVITGPNGGGK
                     STIAKLIMGIEKATSGQIILDGEDITNLSITERAKKGIGYAFQQPPRIKGMTVENLLT
                     LAHGKPLSTDVCCQYLTDVGLCSKDYLNREVDNSLSGGEMKRIEIATLFARDLKVSIF
                     DEPEAGIDLWSFGKLNESFKKIHEESNQTIIIISHQERILELADEIIVLQDGSVKSHG
                     TKEAILPEIMCQVNSSCELMKDMN"
     gene            85326..86243
                     /locus_tag="PC414_000193"
     CDS             85326..86243
                     /locus_tag="PC414_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HDF2462609.1"
                     /translation="MNKIDLNLLEKVAELRGTPLGAYNIRKNGQGVERHSTENVTIIP
                     KTDKPGIDIIVKPNTKGENVHIPVIVTEAGVQDMVYNDFEIGENADVVIIAGCGIHNC
                     GCDDSEHNGIHRFFLRKGARLKYVEKHYGEGDGEGKRILNPVTIVNMDEDSYCEMETI
                     QIKGVDSTKREMDANLGKGAKIVVFEKLLTHGNQVAESNMKVILEGENSSAQVVSRTV
                     GQDNSKQKFNPCVIGDSLCSAHVQCDSIIMGNSQISAIPEIAANHPDALLVHEAAIGK
                     IAGDQILKMMTLGLTEEEAEQQILNCFLK"
     gene            86385..87569
                     /locus_tag="PC414_000194"
     CDS             86385..87569
                     /locus_tag="PC414_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDF2462610.1"
                     /translation="MKNLLIRNGTIISPTDGIKFEKRDILVLHGKIYAIGLNLKEKIR
                     KEKLLTNKEFEIINADNMYISPGFIDVHTHCYKRKLPTGMDSDSIGIEKGSTVIFDAG
                     TAGPSNYYDFKKKYMDECKTEVYSFLNVTNEGLNILNESTYLDVINFDKVESIVEKNI
                     EKIKGIKVKASKFQSNESGIDIIKKSKQVAKKLNLPLVVYIGEYPSYIDEVMNILGKG
                     DVVTPVYGNSTNGILKESGTLRKSVINAKNRGVLFDVCHGVDQFDFKVFKKAIKQGLE
                     PDLISTDMHIKNMKDVNYSLLNVINKIMELGISLEKCIEKVTEYPTKIFDLHGNKGRI
                     KIGGEGDFTIFTMEECNDIIKDYNNNELVLNKKLRLQYTVKSWMKSSEVYRHGYENTI
                     IN"
     gene            87676..87864
                     /locus_tag="PC414_000195"
     CDS             87676..87864
                     /locus_tag="PC414_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HDF2462611.1"
                     /translation="MKAFVDKDVCVACGACIGTCPDVFSMSDEGHSVAIEGEIPADLQ
                     DLCKEAEEGCPVSAITVK"
     gene            88013..88663
                     /locus_tag="PC414_000196"
     CDS             88013..88663
                     /locus_tag="PC414_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDF2462612.1"
                     /translation="MKKNYEIVLFDLDGTLTDPKEGITKSIQYSLNSFGIKEDLENLD
                     QFIGPPLHDTFKEYYKFEDKKAKEAVEKYREYFADKGIFENKIYENMKEILEMLYKNG
                     KILLVATSKPTVFAETILRYFDIDRYFKYIAGSNLDGTRVNKNEVIQYVLDLCNVKDK
                     DKVIMVGDRKYDIIGAKKIGIDSIGVLYGYGSFEEISESEPTYIVENVESIKDILL"
     gene            88766..89704
                     /locus_tag="PC414_000197"
     CDS             88766..89704
                     /locus_tag="PC414_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDF2462613.1"
                     /translation="MKSILIMGGSDFIGSALAKRLIKCGYQIDILTNGKKEIDYNGFK
                     EHLICDRKVRKDMENIITGRKYDYIYDMTAYTKEDVSNLIDFISMDNLKKYIVLSAGA
                     VYKDSGRNIKEENEKGENENWGKYGLNKKEAEDFIINSPIPYIIIRPTYIYGENNNLY
                     REYYFFEKIEKNEKIPVPKGKQVSNQFIYIGDLVKVLESIMKNPHVREAYNVTNPQLI
                     SWDDLIYTCGEIIGKEPIIKYVDMEKVEFRERTYFPFRNIDFNLDINKLIEHGLYIPN
                     VLLKEGLTATYKWFSANKPKMHDRKMNKVESVMQIV"
     gene            89730..90581
                     /locus_tag="PC414_000198"
     CDS             89730..90581
                     /locus_tag="PC414_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF2462614.1"
                     /translation="MNSKIKYFLKFFILIFILIFIYSVAIEPSLLIVKKYNISENGAL
                     IKNDSIESKSESVKVVQISDTQIGSFYSTKNLKKVANKINTLNPDIIVFTGDLIDYSN
                     KNPSVDEITTILSSMNARLGKFSVFGNHDYMYKLPRYYRQIMKNSNFNLLVNENKKIK
                     LKDDKYINILGVDEILNGNPNIKYLESQIDNKSFNLLLAHEPDLVDMLSKDTMNLVLS
                     GHSHGGQIRLPIKGALVTPPYGRKYTKGFYDINGNHLYVSSGLGSTKLPFRFFNIPEI
                     IEFNIII"
BASE COUNT        34923 a        10428 c        15225 g        30082 t
ORIGIN      
        1 atataaatta acctactgtt taattttcaa tgttcgtgtt tatttcttgt tccttttttc
       61 aaggacaagt aatactatac catctttaaa acagtaggtc aatacttttt ttatattttt
      121 ttataaattt ttttcatatt cttttttcag cttctctact acctttttct ctagtctaga
      181 aacagtcatc tgtgatatcc ctagttcttt agctatacta gattgtgttt tatcaaagaa
      241 aaatctatct ttaaaaattt taacttctaa ttcatttaaa gtttcaataa acttattaat
      301 aaaatcttca tactctatac ttccaaagtt accttcttct tttccaatct tatcaataag
      361 agttatatct ttatcctctg aatcatcatt acttgatgaa tccaaagaca ttggttgata
      421 tccataagaa gcctccatag cctctaaaac atcttcttca ctaacgccta tataatcagc
      481 tatatctttt acctttggat gcttcttatt ttcttgttct aatttaatct tcgcatcact
      541 tatctttttg cttaattcct gtattcttct tgggactcta agtgtccata ccttatctct
      601 aaaatacttt tttatttctc ctactatagt tggtgttgca aagctggaga actcaaatcc
      661 tttctcaaca tcatatctat caatagcata aattaatgcc aatgaagcaa cttgatatat
      721 atcttcaaaa tcaacacctt tgtttatata cttcttagct aacaatcttg ctaaatacag
      781 atgtctttca ataagaatat ttctaatgtc tacattttta tttttattat ataaattaaa
      841 tagctctttt gtatccatat ttaggtaatg tgtagcatta gctacatttt tcattaaata
      901 tcaactccta aatatttagt catttttatt tttgtccctt gattatgttc tgattcaata
      961 tctacatcat ccattaaagt tttaattata aacaatccaa gaccactctc ttttggattc
     1021 tctaaatctg gtgtacttat tgagctaaca tcatatcctt ttccattatc ttgaatttct
     1081 atattaattg cattatctaa aatattaaat actatattaa acacatcatc tttactgtgt
     1141 tttattgcat ttgtacatgc ttcagatact gctaccttta tatcttctat atcatctatt
     1201 ggaaaaccca tcttattggc aattccagat gctgttaatc ttattatact aacataatct
     1261 ggatttgtag taatttccat ctttatagtc tcacaagcca aagtttatcc ctccacttta
     1321 aatattttat ctagacctgt tatagtaaaa atctttctta cgttactctt aggatttact
     1381 atgtatatct ctttttcatt tatttttaat ttttttaata cacctatcat agctcctaag
     1441 cctgttgagt ctatataatc taaatcttta agattaatct ttacatctaa catatttttt
     1501 tctattaaag catgtaaatg ttcctttagt ttatctgctg tagaaacatc aagttctcca
     1561 tcaagacaaa cattccaaaa tttattttgt gaatctaaat ttgaatcaat attcatagac
     1621 atgtaaaaac ctcctatttc cttttaataa ttttaaatat atttcctata ctcaatattt
     1681 tattaattac ataaactata tcatctattc cctttgatat tgatgatgca ttttcaacaa
     1741 tatcttgtat atgtctttca ttataatcta caatcttata tgccttttca acaaccttat
     1801 ttatgctgtt tagagtttta gctaaataaa ttgctactat aagagcagag cttcctacta
     1861 acactgctcc aatttgccac ccccatgcat tcattagtta acatctccct catcatcaaa
     1921 gcttttactt attactatat cttcttctga aatctttata tcttcttcat tattatcatc
     1981 atccactaaa tttattattc tctctttaac atcattaaat gtttcatgta gaacttcttt
     2041 tggattttct ttgacatcat taaacttctc ttttgtttct tttcttagtt cagaaccttt
     2101 tttaggtgca aaaaacatac caaatataaa ccctaaaata gctcctaata ataaaaatcc
     2161 acctttttta caacttcttt tattgcacat aacaattact cctttcaatt ttaaattttt
     2221 atctaactac attatacaaa aagaatagga tatttatcct attcttcttc aatatttatt
     2281 ttggcaatcg aaactacatt gacttcatca ttcgtcttca ttaatttaac accacttgta
     2341 actcttccaa acagtgatat ttcattgact cttattctaa taagaactcc atcagaattt
     2401 ataatcatta tctcatcatc ttcgttgacc atatctgcac ctacaattgt accagttttt
     2461 tcagatatat tataagtttt aattccttta ccagctctta tttgacttct atactcttct
     2521 atatttgttc tctttccaaa accattctta cttacaacta acacatctgt acctttactg
     2581 caaaggttca tagacacaac aaaatcttct ttacttaaag ttataccttt tacacccatt
     2641 gctgttctac ccatatatct tatatcattt tcatcaaatc ttatagacat accttcttta
     2701 gttactaaaa gtacttcttg ttttccatct gtaagttcta ctccaataag ctcatcatca
     2761 tctcttaaac ctattgcaat aagaccagat ttatttatat ttttgaactc ttctctctta
     2821 gtctttttaa caataccttt tttcgttgca agtaataaat attcattttc atcattacca
     2881 tcaataggta ttagagtagc aattttctca tctgctgaca attgaagtaa attcactata
     2941 gcagtacctt tagcctgtct cttaccttct ggtatttcat atgcatttaa tttaaatact
     3001 ctacctttat ttgtaaagaa taatagtcta ctatgagtag ttgtagttac caagtgcctt
     3061 acaaaatctt cttctctagt tgtaagtgct gaaatacctc ttccacccct tttttgactc
     3121 ttataagtat cagatggaag cctttttata tatccaaagt gagtaagagt tattgctatt
     3181 tcttcatcac ttataagatc tctcatatct atttcgcctt cagcatgtct tatttctgtt
     3241 cttctctcat cagagtaatt ttcttttatt attgtaattt catcctttat tacatttaaa
     3301 agtaatcttt catcagctaa aatctctttt aatctattta tcttcttgat taaatcttca
     3361 tattcagctt ctatcttatc tctttctaaa cctgtaagtc tttgaagtct catatctaat
     3421 atagcttgtg cttggatttc agttaatttg aatttttcta ttaaacctag cttagcttct
     3481 tgcccagtct ttgaagctct tatcaagctt ataacagcat ctatattatc taaagcaatc
     3541 tttaatcctt ctaaaatatg tgctcttgct tcagctttat ttagttcaaa tttagttctt
     3601 cttgtaacaa catcttcttg atgtttaata taatgatata atatttgttt aagatttaaa
     3661 actcttggct gaccatctac aagtgcaagc attattatac taaaagtatc ttccatttga
     3721 gaatgtttat acaaattatt taatactata ttagcattag catccctctt taattctata
     3781 acaattctca taccatttct attactttcg tctcttaggt ctgatatacc ttctattctc
     3841 ttttctttaa ctaactctgc tattctttca accagtttag ccttatttac ttgataaggt
     3901 atttctgtaa ctattatttg ctgttttcct tttggtagct cttctataaa agctctagac
     3961 ctaactttaa cttttcctct tccagttctg tatgcttctg ctatactttc ttttcccatt
     4021 ataattgcag ctgtagggaa atctggtcct tgaacaaatt ttattaaatc atctacacta
     4081 cactctggat tatctatcaa atatacagtt gcatcaatta cttctgctaa attatgtgga
     4141 ggtattgaag ttgccatacc aacagctata ccatttgagc catttactaa taaattagga
     4201 tatctagctg gcaatactga aggctctttt aacgactcat caaagtttgg tttaaagtct
     4261 acagtttcct tttcaatatc tctcagtagt tctaatgata atttactcat tttagcttct
     4321 gtataacgca tagcagctgg tgaatcccca tcaacagaac caaagttacc atgaccatct
     4381 actaaaagtg ctctagttga aaaatcttgt gccattctta ccatagcata ataaacagca
     4441 gtatctccat gaggatggta cttacctaaa acgtccccaa caatacgagc tgacttcctg
     4501 tatggtttat ctggagttaa atttaactca ctcattgaat ataatattct tctatgaact
     4561 ggctttaaac catctctaac atcaggaaga gcacgtccag ctataacact cattgaataa
     4621 tcaatatacg attttttcat ttcttccgct atttcaatag ggagtatttt gttattttct
     4681 tccatgtata ctaatcccct ctctctatat atctaagttt ctaacaaatc ttgcattttc
     4741 ttctataaat tcttttcttg gagcaacctt atcacccata agcattgaaa atacttcatc
     4801 tgcaattgca gcatcttcta cagtaacttg taataaagtt cttgtctcag gattcatagt
     4861 tgtttcccat aattgctctg cattcatctc tccaagacct ttgtaccttt gaagttcaac
     4921 accatttctt ccaatttcat ctagtaagat atttaactct ttatctgagt aaacataatg
     4981 ttccttcttt tgctttgtca ctttatataa aggtggctgt gctgcataaa catacccctc
     5041 atctataaga ggtctcatat atctaaagaa gaatgttaat aataaagttc ttatatgggc
     5101 tccgtctaca tcagcatcgg tcataattat aattttatga tacctagcct tatctatatc
     5161 aaaatcttct ccaataccac aaccataagc tgttatcata ttttttattt catctgaaga
     5221 tagtatccta tctagtcttg atttctcaac attaagtatt ttccctctta atggaagtat
     5281 agcttgacta tttctatctc taccttgttt agctgaacct cccgctgaat ccccttcgac
     5341 taagaatatt tcacttttag atggatcttt ttctgcacaa tctgcaagtt ttccaggtaa
     5401 agatgtactt tctaatacac tttttctcct tgttaattct cttgcttttt ttgcagcttc
     5461 tctagctctt tgtgctctta aagctttatc aactatgatt cttgctgttg atgggttttc
     5521 ttctaggaaa gaccccagtt catcaactgt tacactatca actatacctc tcataaagct
     5581 attacctagc tttgttttag tttgaccttc aaattgaggt tctggtaatt taactgatac
     5641 tactgctgta agaccttctc ttatgtcttc accaagtagg ttaacatcat tttcctttaa
     5701 aaatttattt ctcttagcat aatcatttac agttttagta agagctgctt taaatccact
     5761 taaatgagaa ccaccttcat gtgtatttat attgtttgca aaagaatata tgttctctgt
     5821 ataaccatct gtatattgca tagctagttc aacaaaacaa tcatcaacct ttttatctat
     5881 ataaactatg tcatcatgta taccagttct agatttattt aaatacttaa tatattctac
     5941 caaaccacct gtatagtgaa attcttttct tttttcttgt ccttctcttt tatcttcaaa
     6001 tactattttt attcctttat tcaagaaaga aagttctcta agcctatact ccaatgtttc
     6061 atatttaaac tctatttcat caaatattgt ctcatctggc ataaattgaa tcattgttcc
     6121 tttatcttga gattctccaa ctacttcaag cttagatgtt ggcaatcctt tctcatatgt
     6181 ctgcttatat atctttccgt ttaaataaac ctctgctacc atccattttg acaatgcatt
     6241 aactacagaa acaccaactc catgaagacc tccagatact ttatatcctc cgcctccaaa
     6301 ctttcctcct gcatgaagat tagttaatac tgtttctaaa gttgatttac cagttttagg
     6361 atgtatttca acaggtatac ctcttccgtt atcttttact aaaacactgc catcttcatt
     6421 aatagataca tatatgtctg aacaatatcc ttgtaaagcc tcatctatac tattatctac
     6481 aacttcataa actaagtgat gtaaacctct agggcttgta ctaccaatat acatacctgg
     6541 tctttttcta acggcttcta atccctctaa tacttgtatc tgacttgcac cgtattcttg
     6601 tttcatttaa tttcctccat tctctatgct aataacatcg ccattttcaa tattaaatat
     6661 tgtaacattt ttttcatcaa atattttttt atgtgaaact tcagcacttg ttataaacat
     6721 ttgaacattg cttaaattat ttactaaaag tttttgtctt gcttcatcta actcactaaa
     6781 aacatcatct aaaattaata ctggatattc ttcaacttca ttttttatta attctatctc
     6841 tgataacttt aatgatattg aagcagttct ttgttgacct tgtgaaccaa aaagtcttgc
     6901 atctaaatca tttatgaata tatttaagtc atctttgtgt ataccatatc tggtagtctt
     6961 agattctata tcattaggtc tattcgatga caacttggct aaaaatttat tatatactgt
     7021 atctatagta tcttcatcag ttatatttat ttgattttta tatcgaattg ataatctttc
     7081 aactccatta gtcaaattca tatgcatatt ttcagatata ttagctatct tttttataaa
     7141 gtctctacgc aaaatataaa tataacttcc atactttgct aacgtatcat catatacatc
     7201 taataaagct tcatcaacat gtatattttt aagcacccta ctcctttgag aaagtgtttt
     7261 attataattg gtaagatatt tataatattt aggtataatc tgactaatct ctttgtctat
     7321 aaatgccctt ctttctttag gaccctcttt aaccaacctt aaatcctcag gagaaaatat
     7381 aacaacattt aagttgccaa gtagttcttg tatcttttgt aaaggaactt tatttattct
     7441 tatccctttt ttatcttttc caataattag ctctattaga ctatatttat tatattttga
     7501 aaaacttcct ccaatatata agttttcact attaaatcta accatttctt tatctttgtt
     7561 agtcctaaat gattttccaa aagaaagcat ataaatagat tctactatat ttgtctttcc
     7621 ttgaccattt ttaccaacaa gtagatttac tttcccatta aattccagat gcaacttttt
     7681 ataatttcta aaattaacaa gttgcaaact tttaagtttc acaaaaagtt ctcctttttt
     7741 tacaatactt ttattttagt tccttctatt tcaactatat ctcctgattt tatctttttt
     7801 cctcttctta attctatttc atcatttact gtcactaaac cttcttgaat taaaaatttt
     7861 gcatgacctc ctgttgatgc aatctctgct aatttaagaa attgatccaa ctttatatat
     7921 tctgattcta tagttatttc agtcataata gttcctcctt gtcactattt taaacaaatt
     7981 cctattgata cactaaaagt atattatacc ataaatatgc ttataaaagg agttgtctaa
     8041 gattagaaaa attttctaca aaagacaact ccttcatgtc tgctatatat ttgatgatat
     8101 tcttactgga agcagtaagt aaatataatt aacatcatct gttggtttaa ttatacaagg
     8161 atttacattt gtagtaaatt ctataaatat ttcctcatta tctatatttt tcaaaccttc
     8221 gataaaatat ctagaattga aagctatatc taggtaatct ccatctaaat ctatttcaac
     8281 ttcttcatac acatttcctt tttctgtatt agaagtaatt gccattactt tatctcttat
     8341 agataacttt ataagattat ttttttctga ttgagacaat aaagacgctc tctctatact
     8401 atttaaaagt tccttagtat ttaatttaac tctgctgtta tgttctcttg gcaataattt
     8461 tttataatca ataaaatctc cttcaagcaa tcttgtaata attttcgtat cattaattat
     8521 aaaaatagca tttttttcat taaatccaac tttaacatta tcctctccag aaagcaaact
     8581 atttacatct ataagtgtct ttccaggaat tatgactttt atattttcag ttaaactatc
     8641 aaccgaacaa ctttttacag ctaatctata tccatcaatt gctactaaat ttaagtttct
     8701 atctacaatt tctaaaagtt ctcccattaa aacaggtttt gtttggtcct gtgagatagc
     8761 aaatacagtt tgtttaatca tattttttag gatttcttga ggtatgctat ataaatcttc
     8821 ttcatttaat tctggtaatt taggaaattc ttttgctgca taacctttaa ttttaaatct
     8881 tgaatttaca caatttatgt aaatattatt ttctgaatca gtttctatct caacaaagga
     8941 atctggtaat tttctaatta tatctccaaa tagccttgca tctacaacaa catcaccttt
     9001 ttctatgatt tctgcttgta cataagtttc tattcctatt tcagcatcat atccagtaag
     9061 ttttaattga ccttcttctg tagatataag tatacctttt aataattcta ttgttgtttt
     9121 tccattaata gctttttgag caattccaat tctatttgct aggatttttt gattacaaat
     9181 tattttcaat gtggataacc tcctctggct ttttatatat aaaaataagt taaaatcata
     9241 gtagtaatag tagtaggtgt tgttgataat gttaataagt atgtataaca ttataaatac
     9301 aaagatatta acatgtgtat aagatgttaa tatctttgtt attttagact tgataatttg
     9361 ttaataattt ttgttcaaaa aaagtattaa caggttattc aagagatata aacatacaat
     9421 tgtgtataaa ttatcccttc aaatctgata taattttgtc tattttagtc ttaatttctt
     9481 cactttcttc tatatcctta gatactttgt catgagcatg aattactgtt gtatggtctc
     9541 tacccccaaa ctcttcacct attttaggaa gggataaatc agtaagctcg cgcgttaaat
     9601 acatagctat ctgtcttgga taagctattg aacgtgttct ttttttagaa ttaaaatctt
     9661 ccatctttaa attgaataca gaagatactt tttcttttat tctgagaaca tttatttctt
     9721 catttttaga agttgttata atatctttta aagcttctgt agcaagatca aaagatatta
     9781 ccctattgct aagagaagaa taagcaacca ctctagttaa tgcaccttct agttccctta
     9841 tgtttgactt aatatttttt gctatatatg acataacctc atttggcaca tctatacgtt
     9901 ctagttgagc cttttttcta agtatagcaa ttcttgtctc aaagtcaggt gcttgaatat
     9961 cagttataag tcccatttca aatctcgatc ttagcctatc ttctagtgta ggtatgtctt
    10021 ttggcggtct atcactagat ataattattt gtttgtttgc ttcatgaaga gtattgaatg
    10081 tatggaaaaa ctcttcttga gttctttctt ttcctgctat aaattgaata tcgtcgatta
    10141 gtaatacatc tacgtttcta tatttattcc taaattcttc gtttttatcg tcttttatag
    10201 agtttataag ttcattagta aatttttctg aagaaacgta aactactttt gagtcttttt
    10261 tttgactaac aatatgatga cctatagcat gcattaggtg agtcttacct aatccaactc
    10321 ctccatacaa aaaaagtggg ttataagctt tagctggaga ttctgcaact gcaacgcatg
    10381 cagcatgtgc aaatctatta ctattaccaa taacgaatgt atcaaatgtg tattttggat
    10441 ttagattggg gtaaagaact ctataattta aatctgtgct atcagaattt aaatcagcaa
    10501 cctctttttc gctcaataca aatttaatat tatatttttt taatgacagt tgactaatag
    10561 catcttcaat taaatgtaaa tatctattct ctaagatatc tttattaaaa tcactaggag
    10621 ccagtaagat taaatcatta agatgtattt ttaatggtac gatgttttta aaaaaggtat
    10681 taaaacttac tgaagttaag tcacctttta ttaattgtag ggttttgtcc cataaagaaa
    10741 ctatatccat aatataaacc tccgaacaga ataattaata ttttccacaa agttattaac
    10801 agaaaagcaa aaatatatat aaattttaca gaataactaa atttatggat aatggtgttg
    10861 ataaattaat aatagtataa attattaaaa aaatatgaaa agatataaac aatatgtaga
    10921 taaagttata aataacataa acatacaata aatggtgatt ataactagag tattgtcagt
    10981 aatcttaaaa gttatacaca aaagtatcca caagctgtga ataatttcta ataagttgat
    11041 aacatttatg cacattatca acaagtatat ttataatatc aaaatatatg tcaaacttca
    11101 atgatttgta actaatttat ccacaatatt aactaattgt tgataaaaat ataaacatgt
    11161 taaaattact ttatttgtgt atataattga aaaaaataat ttagaataaa attaaataga
    11221 tttttactag tattttcaat aaaaaaaata cataatatat atatggtaga ttttgtgata
    11281 taagcgtccc aaaaactcgt tgacaacagt agtaaaagta tttataatta tatgtgttat
    11341 gtttactttg agggtaaagt tattattttt ataaacggaa aataaaatct gaaaggcggt
    11401 gcaaaataat gagtaaaaga acttatcaac caaaaaagag acaaagaagt aaagaacatg
    11461 gtttcagaaa aagaatgaag acgtctaacg gaagaaacgt gttaaagaga agaagagcta
    11521 aaggaagaaa tagattaact cattaattac aaaagggccg ctgcaaaggt ggcctttttg
    11581 tgtaaataat tacaattcat agctgtgttt atggaggtta actatggact ttaataggac
    11641 taaagggttg aaaaaagact ctgattttag aaaagtatat aaacacggca aatcttttgc
    11701 aaacaaatat ttagtaatat atatacttaa aaataaatca gattatagta gagtaggtat
    11761 ttctgtttca aaaaaagtag gaaaagctat tactaggaat agagtaagaa gattaataaa
    11821 agaagcctac agattaaata ttgatgaaaa aattaagcct ggatatgata tagtatttat
    11881 agcaagggta tctagtaagg atgctacttt taaagacata gacaagtcta taaaaaattt
    11941 agttaaaaga acggatattt ctatttaatt gtatgctata aatatggagg ttaatgactt
    12001 tgaataaaat aatttatata ttaaaggaaa taagtaagta tttatcgaat ttatgtattt
    12061 atttagtgag attttatcaa aagtatatat ctcccttaaa gggacctacc tgtagatttt
    12121 atccaacttg ctcacaatat tcaatagagg cgtttaagaa atatggattt gtaaaaggta
    12181 tgtatttaac gataagaaga attttaaaat gtcatccttt tcatccagga ggttacgacc
    12241 ctctaaaata aaagagagta actattagga ggtataaaaa aattgaatat aataggaaat
    12301 ttcttaggat tgattcttaa ggtaatattt gaatttgtta atcattatgg tatttctata
    12361 atacttttta cgatacttgt aaagattatt ttattaccat taacaattaa gcagactaag
    12421 tcaacaaagg caatgcaaga tatacaacct cgtataaaag agatacaaga aaaatataaa
    12481 aataagccag aaaaacaaaa tgaagaaatt gttaagttat atggagaagc taaaattaat
    12541 ccactatctg gttgtttacc tttattaata cagtttccta tattaatagg attatttagc
    12601 gttttgagag aaccagttgc tcatggcgtt tttgcaaaca aagctgcatt tttagcagct
    12661 gacaatgggt ttttatggat aaaaagctta acatcaccag attatgtatt agcagtattt
    12721 tcaggagcaa gtgcatatgt catgcaaaaa gtaatgacac caaaagatca gttacaaggc
    12781 tctatgaaag taatgacata tgtaatggca ggtatgtctt tctattgggg atttatattc
    12841 ccagcaggac ttacattgta ctggacagta agtaacttat tctcaatagc tcaatattac
    12901 ttaataatga atcctttaaa agctaaactg gctgctaact ctaaggagga aaaagttaat
    12961 gagaaaaagc ctaaaattaa taaaaagtaa gagtaaagag gaagctataa gtaaagctat
    13021 agcagagttg aatttaaaag cagaggatat agaagtagaa atacttgaaa atccaagtaa
    13081 agggttttta ggttttatag gtgctaaaga tggtacttat gaaatttttg ttatcgagag
    13141 ggaagtagat gtagctaaaa attttataga ggtaatgttg aaaaatgcaa atgttgatgc
    13201 caaagtcaat gtatctcaaa aagataattt aatcatggtt gatattgaag gcaaagaagc
    13261 tgcaagttta attggtagaa gaggggaaac tttagattct attcaatttt tgactggatt
    13321 agctcttaat aaaataaata aagattctca tacaagagta cttgtagata tagaaaatta
    13381 tagatcaaaa cgagaagaat ctttaataag atatgctaat aaagttgcta gagaagtagc
    13441 gaaaacaaga aaaactaaaa aattagatta tatgaatcca tatgagagaa gaataataca
    13501 ttcagctctt caaaatgaca aatatgtaat tacatatagt gaaggaaccg atccatatag
    13561 aagattagta attgagtgta aaagataaag tcaacctcct aaacattagg gggttttatt
    13621 ttttaatatt attaaatcat taatgattgg ttaaatgtaa atctatatgt aaaaaattat
    13681 gaattaagag gtgataaatt tgtttataga tgatacaatt gcagcaatag ctacagcacc
    13741 tggagaaggt ggtataggta tacttagaat aagtggagaa aaagctttga aggttgcaga
    13801 agaaatcttt aaatctatgt ctggaaagag tattgaagaa tacaataaga gaacattaat
    13861 atatggaaat atagtggata atgaaaatat aattgatgaa gttttattgg catatatgaa
    13921 aggaccaaat tcctatacag gagaagatgt tatagaaatt aattgccatg gtggatttat
    13981 ttcggtaaag aagatattag aattaatact atcaaaagat gttagattag cagaggctgg
    14041 tgagtttact aaaagagcat tcttaaatgg aagaatagat ttatctcaag cagaggctgt
    14101 aattgatgtt ataaaagcca aaactgatat agctcatgaa gttgctcaaa atcaattaga
    14161 aggttcttta tcaaagaaga taagagaatt gagagataaa gtcactgaga tattagctca
    14221 tgtagaagtt gctatagatt atcctgagga agatattgag catataacat atcaaactct
    14281 taaagaaaaa actgatgaac ttaaaaaaga cattaaaaaa ttatatgata cagctgaaag
    14341 tggaaaaata cttagagaag gcttaaagac ggtaattgtt gggaaaccaa atgtaggtaa
    14401 atcttcacta cttaattcta ttttaggaga gaatagagca atagttactg atataccagg
    14461 aactactaga gatgttatag aagagtttgt aaacataaaa ggtatacctt taaaaattgt
    14521 tgacacagca ggtataagag atactgatga tattgttgaa aaaataggtg ttgaaaagtc
    14581 taaagaatca tttactagtg cagatttgat agtaatggtt ttagatgcat ctagaaagtt
    14641 gtcagaagaa gatatagaga tattagaaaa acttaaggat aaacaaacaa tagtgctgtt
    14701 aaataagaat gatttgaaac aagagattga agaagaaaaa atacttaaat atgtagaaaa
    14761 taatagtata ataaaaatat cagctttaca acaagaagga atagaagaat tgcaagataa
    14821 aatagagtct atggtatata aaggtagtat caaaaataat tctagtttag tagtaacaaa
    14881 ttcaagacat aaagatgcac ttagtaaagc atataaatct gcaacagatg ctttaattgc
    14941 tttggaacaa agtatgccat ttgactttgt agaagtcgat ttaaaaaata tatgggacta
    15001 tcttggatat attaatggtg atacagtaac agaggattta ctagataata tattccataa
    15061 tttctgtatt ggtaagtaat gtacaaaagt aaacttgttt ttattataga gatttttaaa
    15121 aaatttgaag tgtttctcat gaaacattaa ttttgaaaag aaaggaatta taaatgataa
    15181 agtttgaagc aggaaaatat gatgttatag tagtcggagc aggacatgct ggatgtgaag
    15241 ctgctttggc tactgcaaga atgggatata aaactttgat aataacaatg tctttggatt
    15301 ccatagcatt aatgccttgt aatccatcaa ttggagggac tggtaaaggt caattagtta
    15361 aagaaataga tgccttaggt ggtcagatgg gattaaatat agacaagaca tatatacaaa
    15421 gtagaatgtt aaatactgcc aaaggtccag cggtacactc tttaagagca caagcagata
    15481 aatttaaata tcatgaagaa atgaaaaaga cattagaaga tgaaccaaat cttgatatag
    15541 ctatggatga agttgtagaa atacttcatg aaggaaatgt tgtaatagga gttggaacta
    15601 aactaggatg ttcatttaaa tctaaagctg taatattggc cactggagtt tatctgaact
    15661 caaagatata tatgggtgaa gttgcatttt atgaaggtcc taatgcattg gggtatgcaa
    15721 aatatcttac agatagctta gttgagcttg gccttagaat gagaagattt aagacgggaa
    15781 ctccagcaag agttcataga gatagtatag acttctctgt aatgtcttta caagagggtg
    15841 atgaaaaagt aactccattc tcatttatga atgaaaatat agaaaaaaaa caagaacctt
    15901 gttatttaac aagaactaca gaagaaactc aaaaagttat actggataat ttaaaaaggt
    15961 ctgctatgta tagtggagta atagaaagta ctggtccaag atactgtcct tctatagaag
    16021 ataaagtagt aagatttagt gataaaacat cacatcaatt atttatagaa ccagaagggt
    16081 taaatactaa agaaatgtat atacaaggaa tttcaactag tttacctttt gaagttcaat
    16141 tagatatgta caaaactata aagggacttg aaaattgtaa gataatgaga cctgcatatg
    16201 ctattgaata tgattgtgtt gacccaactc aactaaaaat aagtttggaa ataaagggtg
    16261 ttgagaactt atttagtgca gggcaattta atggaacttc tggatacgag gaagcagcag
    16321 ctcaagggct tatggctggt ataaatgcag ttagaaagat agaaggaaaa gagccatttg
    16381 tattagaccg ttcagaagct tatataggtg tcttattaga tgaccttgtt actaaaggaa
    16441 ctaacgagcc ttacagaatg atgacatcaa gagcagaata tcgactatat ttaagacaag
    16501 ataatgcaga tatgagatta actcaaaaag gttatgatat aggattagtt aaaaaagata
    16561 gatatgaaag gttcctaaat aaaaaggctg ctgtagagaa agaatttgaa agactaaaaa
    16621 atgaaagagt tacaccaaaa gaagtaaata gtttgttaga agaaaaagga gcaacaccaa
    16681 taaaggttgg aatatctcta tatgaatttt taaagagacc agaggttact tacgaattgc
    16741 ttgaagaatt agggaaaggc gctggagaag atgtttcaag agaagttaaa gaacagtgtg
    16801 taataataac taaatatgaa ggttatatag agaaacaatt gaaacaaata gaccaattta
    16861 aaaaacttga gaataaaaag ctggatgaga aaataaatta ttcatctata gaaggtctta
    16921 gattagaggc tagacagaaa ctagatgata taaaacctat ttcaataggg caagcatctc
    16981 gtatatctgg tgtttctcca gctgatatat cagttttact tatataccta gaacaaatca
    17041 gaagaactag aggtggcaaa ggtgaataat atagaacttc tgaaaaacgg gattgaaggt
    17101 tttaatatag atacgaatga cagtatgtta gaaaaattta aaatctatag agagatatta
    17161 gttgactgga ataaaaagat gaatcttact ggaatagaag atgaaaaaga agtgtatata
    17221 aaacattttt tagactctat ttcagctgta aagaatggat atataaaaaa tgggatgtct
    17281 ataatagatg ttggtacagg tgctggtttt cctggaatac cattgaaaat atgtttagat
    17341 agcttagaac ttactttgtt agattcgtta aataaaagaa taaacttttt agaggaagtt
    17401 agtagagtac tagagttaga taatattaca tttatacatg gaagagctga agattttggt
    17461 aaagatgaag actataggga aaaatatgat gttgctacgg caagagctgt tgctggatta
    17521 ccgattctta tggaattttg tgtaccattt gtaaaagttg gagggtattt tatttgctta
    17581 aaaggtccga atgctaattt agagctagaa gaatcacaaa aggctattga tgttttagga
    17641 ttggagtata ttgaaaaaat agatgtagag ttaccagagg tagatttaaa tcataatata
    17701 ttagttttta aaaaagtgat ggaaacacca gttaagtacc ctagaaaagc tggtaaacca
    17761 gccaaaaatc ctattaagta atgaaaaatt tattattggt aagcttaatt atttctcagg
    17821 aaataattaa gcttactgta aaaaaacaaa aataaaggaa ttattaaagt agatgcagaa
    17881 ataaatttgt atgtacatat agagaacaaa aataatttat atctaaaata agagaggtat
    17941 gttatggaag ataaaagagt tatggagata cctatagaag atgtagttcc aaatccatat
    18001 caaccaagaa aaatattttc acaagtttca ttggaggaat tgagcaattc tataaaggta
    18061 tatggtataa ttcaacctat aacagttaga gcaaaagatg gcaaatatga actaattgca
    18121 ggagaaagaa gattaagagc agctaaatta gcagagctta aaaccatacc agcaattata
    18181 aataatatga acgatgaatc ttctgctgtt ctagcgttat tagaaaattt gcaacgagaa
    18241 gacttaaatt tcattgaaga agcaattggt tatgaaaact taataaaaga acatgctttt
    18301 actcaacagc aattagcaga aaaattgggc aaaaatcaat ctactgtagc aaataagctt
    18361 agaatattaa agcttccaaa cgatataaaa atgaagctaa tagagaataa tttaacagaa
    18421 agacatgcta gagcatttct taaactacca agtgaagatt taatgcaaag tgtgttagac
    18481 aaagtcataa aaaatgaatt gactgttaaa aaaacagaaa aattgataca agatgtatta
    18541 gaagaaacta aagttcaaga agagcctgat aaaaaacaga atataaaggg tgctatgagt
    18601 ataagaattt acataaatac tataaagcag gcctttgatg ctatatcaaa tacaggaatt
    18661 gatgctaagt ataatgaaat agataaggga gattatatgg aagttgttgt aaaaatccct
    18721 aaaaaataga gattactgct taccagtaat ctttttttgt aatataagcc ttgtaatata
    18781 aattgattta tagacattat ttatacttaa aaaacaccta ttcaacattt aaaattaatt
    18841 taattcttaa aggggggaac atcatgggca aagttatagc tgtattcaat caaaagggtg
    18901 gagtcgggaa aactacaaca aatgtaaatt taagtgctag cttaggaact ttgggaaaga
    18961 aaatattagt attagattta gacccacaag gaaatacaac tagtggatat ggtattaata
    19021 aaaacgaagt agaaaataca atctatgaaa ttatgttaga tgggttacat attaaagaag
    19081 caataataag tactgaattt gagaatgtag atgtagttcc atcagctact gaactatcag
    19141 gtgcagagat agagcttaca tctaaaacta atagagagta tattttaaaa aattccataa
    19201 aagcggtaat agatgaatat gattatatat ttttagattg tccaccctca ctggggatgc
    19261 ttacaataaa ttgtttaact gctgtagata gtgttttaat tccaatacaa tgtgaatatt
    19321 atgctttaga aggtgttagt caattaatgg agactataaa attagtaaaa tcaagattaa
    19381 atgctgatat agaaattcaa ggtgttgttt taagtatgtt tgatggaaga gctaatttat
    19441 caatacaagt agttgaagaa gttaagaaat actttaaagg tagtgtatat acaactttaa
    19501 ttccaagaaa tgtaagactt gcagaagctc caagtcatgg aaaacctgta atttattatg
    19561 ataaaagatg tagagggtct gtagcttatt tggagctagc agaagaattt atagatttag
    19621 aagaggagga atggtagtat ggagaataag tctaaaagaa gtaatagatt aggtagaggt
    19681 cttagtgcat tgataccaga aattaaagga gaaacttcag aaaaagaaat agtcaacata
    19741 gatatagaca aaatatatcc aaacgaagtt caacctagaa agcaatttga tgaagaaaag
    19801 ataaaagtat tgtcggattc tataaaaaat tatggtgttt tacagccaat agtggttaaa
    19861 atggatgaaa ataataaata tatgataata gctggagaaa gaagatttag agcatcaaaa
    19921 cttgcaaata aaaatcaaat acctgctata attaaagata tagatatgaa agatataatg
    19981 gagatagcct taattgaaaa cttacagagg gaagatttaa attcaataga agaggctcta
    20041 gcatataaga gtttaataga gcattacaat gtaactcagg aagaaatatc agaagctgtt
    20101 gggaagagta gacctcatat aactaataca ctaagacttt taaatcttgg acaagatgtt
    20161 atagaaatga tagacagtgg tagaatcaca gcaggccatg gtaaggcttt attgagaatt
    20221 gcagataaag atttgcaact tcaaatagcg aaaaaaatag aagaagaaga actttctgta
    20281 agagaagtcg aaaatatagc taaaaaaata tctgaaaata aacaagaaga acctaaaaaa
    20341 tctaaaccta aggatgtatt tattttagat gttgaagata aacttagaaa tatatttgga
    20401 acaaaggtga atatttctaa aggaaagaaa aaaggtaaaa tagagataga atactataat
    20461 gatgatgatt taaatagtat tgtatcaatg cttcttgaat agaacaaaaa tataaaacag
    20521 agaggaagat tgttatgatt tatttagata atgctgcaac tacatatcca aaaccagaaa
    20581 gagtgtataa tgctgtttta gattgtatga aaaattattg tgcaaatcca ggtagagcag
    20641 gccataaact tgctatgaga gctgctaggg agatatatga tacaagagag aatatagcta
    20701 agttgttcaa tgtaagtaat cctatgaata tagtatttac atctaatgca acagattctt
    20761 taaatttagc gataaaaggt gtattacaag aaggagacca tgtaataaca acgtctatgg
    20821 agcataattc agtaataaga cctataaaag cattggaaaa aaggggtatt gaaaatactg
    20881 tagtaaaatg cgattatgag ggatttttag attatgaaga tttagaaaaa tcaataaaga
    20941 gtaatactaa actgattgta acaacacatg cgtctaatgt atgtggaact ttaatagata
    21001 taaaaaaagt tggagaaata gctaaaaaac ataatatact atttttagta gatgcatcac
    21061 aaacagcagg tgtttatgat atagacgtaa atgaatgtaa tatagatatg ttggcaatgc
    21121 caggtcataa atgcttattt ggacctcaag gaacaggtat attatatgta agagaagggt
    21181 tgaacttaaa tatattaaaa gaaggtggaa caggtagtaa atcagaagaa atagttcaac
    21241 cagagttatt cccagataaa tatgaatcag gaactcataa tactccaggt atagcaggtc
    21301 ttaaccaagg tatactattt atatttgaaa gaggaatcaa caatataaga caacatgaag
    21361 aagaattatg tcaatatatg atagacaaat tagaggaagt accagatata aagatatatg
    21421 gaccaaagga cagtaaaaaa agagcatcag taatagctct aaatatagga gatatggatt
    21481 ctggagaggt tactttctta ttagatagtg attataatat agcgactaga tcaggaatac
    21541 attgttcacc actagcacat accactttag gtactttaaa acaaggtgca gtaaggttta
    21601 gtataggata tttcaacaca aaagatgaga tagataaagc agtggaagct ttgaaaaaaa
    21661 tttcaaaaaa taaatagcat gagatttaat aatatagaaa taaaaaaagg ggatatttca
    21721 aaataaaaat ttgaatcatc tccttttttg tacaaaaaag aaaaggggtt cctaagaacc
    21781 tctaatctat ttgataatag tttcaagaaa ttactttaag taatctcctt cttcaagaac
    21841 tatatgacca ctttcgtaat tagaaccatc aacagtaatt tttatttgtt caacattcat
    21901 attttttatg taagtacgag caactgaatc aagcattaaa gtttctgcat cactacctaa
    21961 gtttgacttt gtaaagtcag agctaacatc taatatacca gtttttacac catcaatgtc
    22021 ttttgtagta aatgaattta attttgcaga ttctggaaca actttaagtt tttgaagttc
    22081 ttcaaataaa gtgttctcat caattttatt taaatcaact ttattttcaa ttaaatctgt
    22141 cttatctaca tcaaaagagt aaatagtagc tgattgagtt ttagtttctt cttttggagt
    22201 aggtttagct ttgttattag gaacctcttt ttcagtattg ttttcttctt ttggttgctc
    22261 aacatttgta ttgctcttag tttctttttt agatgaatca cttccatttt gagcattagt
    22321 acaaccaact gcgaatatag atatagataa tatacttaac actaacatta ttttcttatt
    22381 tttcataaaa atttctcctc tcttaaaaac tattcttata tataattata gtcgttatga
    22441 gcaaatatat gaatacaaaa tagtaacaaa tttaaaaaat gatttattta aattaataaa
    22501 actcaaaata aactaaattt tagtcatttt tatgggaaat catttaatgt acagtgaaaa
    22561 tattgtaaaa taaaagtagt gtttttaaaa taaatataag ttataattat attataaaaa
    22621 aatgtcttca aaaggtggga atagtatgga aattttgagc ttaggagaaa agattaaaaa
    22681 attaagaaaa gaaaaaaaca tgacattaaa agaattagct ggagatagga taacagcagc
    22741 acaaataagc catatagaga gggataaatc tcatactagt tatgaactgt tggaatatct
    22801 aggcgataag ctagatgtaa gtgtagaata cttattagag accaaggaaa tgcaatcaaa
    22861 aaagataact gataatttaa tactacagag cgaaatttat ataaaagaaa atgaattaaa
    22921 caaggctgag gaccaaataa aagaagtttt agatatatgt aagagatacg atttaatgga
    22981 gaactatggc agatgtaatt ttttaatggc taatattaat ttgaaaaaat ctaattataa
    23041 tgatgctata gaaaactttg aaaaggcatt gtacttcttt ataaaaaata gtgatagtga
    23101 aaatatactt aagtgttatt taaacatagg gaaaatatat atgcaggaag acttttataa
    23161 aggtgctata agccacttta attttgcaga ggaaattttg tcagagaata aatttgagga
    23221 tgttaacata cacaaggaac tatatagtaa gatggcatat tgttacataa aactagataa
    23281 tccaaatatg tcattgaagt acatagaaaa gataaatgaa atagataata gaaatgatag
    23341 aaaagaagat gtcgatattt tggtgttaaa agcaaataat atgcttgaga ttggaaaata
    23401 tgaagaatca agagattact ttaaaaaagc actaaaaata cttgagagtg aggataataa
    23461 aactggatta gcaaatgtgt atttaacaat ttgtgatgtt tacagaaaaa taggtgaaac
    23521 agatagagta cttgaatatt cacaaaaggt atatgacata aagaaaaatg atgaagatga
    23581 atacatgatg tcaggattat ttaagataat agaagcatat atagataaag aagattatga
    23641 tttagctaga aaatattgta agatagcctt agcttcatca ataaaaaata aaaataaatt
    23701 caatgaatat aaagcattga gattctatgc taatatgtat aaaaatcaga atgaagtaac
    23761 tcttgcaatt gaatatctga ttaaatgcat aaaaatagta tcagatttag gcaacaatag
    23821 aatattggcc aatttatata tagacttagg tcaattatat tcaaatatat caaaagaaaa
    23881 agagttagaa tattatcaaa aaggtgtatt tatgtacaaa aatttagaaa taatgtaggg
    23941 taaataaacc tataagggta tagaaacaat ctataacaag caagataata tctatgataa
    24001 aatataaatt gtatgaatat ttttaaggag atgaatcaga caatgagtat aaaaatagat
    24061 gcgagagggc ttgcttgtcc taaaccagtt ataaatacaa aaagagaatt ggataaccta
    24121 gaagaaggtg ttgtagttac aatagtcgat aatgaaacag ctaaggagaa tatattaaaa
    24181 cttgctaaat ctttaagttg tgaagcaaat attgttgacg aaaaagagaa ttttatatct
    24241 atagaaatta gaaaaggtaa aaatatacta gataaacaaa atgttgtaga taataaagaa
    24301 agtgaattag acaatacatg tatatttata tcttcagata agatgggact tggaaatgac
    24361 gaattaggaa aagtgttaat aaaaggattt atatatacat taacagaatc taaaccttat
    24421 cctaagtatg tacttttagt taatggagga gtaaaattaa gtgctgaaaa tgaagaaacc
    24481 atagaaaatc taaaaatact tgagaatatg ggtgtagaag ttttatcttg tggtacttgt
    24541 ttagattatt ataatttaaa agacaaatta caagtaggta cagttacaaa tatgtatact
    24601 atagttgaga cattaaagaa tgcatcaaat accatttcta tataatgtat gtttgtttaa
    24661 taatataaaa ttaataataa agattaactc aaatagatag tcagaaagga ttaagtaatg
    24721 aatcaaatgt atatagtgtc atttaattcg acacatcatg cgataagatc agaaaagtta
    24781 tttggtgaaa atagtctaaa agtaatggct cttccaacac caagagaaat aacagcaagt
    24841 tgtggaatat caattaaatt ttcttttgaa gatatggaaa agataaaaac tattttagtt
    24901 gaaaataatg tagatataaa aggaatttat tgtataagta aattagaaaa tggctcaaaa
    24961 gaagtagaga agttagatta ggaggctaat tatttatgcc aatggattta aaaataggtg
    25021 atattgttga attaaaaaag caacatgcat gtgggtgtaa agagtttgaa atcataagaa
    25081 cgggaatgga tataaagata aaatgtacta aatgttcaag gctaataatg cttgatagag
    25141 agaccttaga aaagagagta aaaaagctaa taaaaaagtg ataaaaattt aacacttaaa
    25201 aaatgtcaaa taccgtatat taatatccaa aattttggta taataagaat tgaagaaaac
    25261 attttcatgg gggtgcttta attatggcag ttaaatatgc aaaaagaatg caaggattac
    25321 aaggatcaga aatacgtgag cttttaaaac ttactcaaca accacaaata atatcttttg
    25381 ctggtggaat gccggctcca gagttatttc cagttgagga aatgaaaaaa gtatcagttg
    25441 cagtgcttga agaaaatgga agatcagcta tgcaatatac tacaacagaa ggatatgaac
    25501 cattaagaga aaaaatagca gctagaatga atgataaaaa caaaactaat gtaaacaaag
    25561 atgatatatt agttacaagt ggttctcaac aaggtctaga ttttgcagga aaagtattta
    25621 ttgatgaggg tgatgtaatt ttatgtgaaa gtccatctta cataggagct ataaacgcat
    25681 ttaaatctta tcaacctaaa ttcatagatg ttccaacaga ttctgatgga atgataatgg
    25741 aagaattaga aaagatacta gaaacaactg atagaataaa aatgatttat gtaatacctg
    25801 acttccaaaa tccaacagga agaacttggc cacttgaaag acgtaaaaaa ttcatggaaa
    25861 tagtaaataa atttgaaata ccagtaatag aagataatcc atatggagat ttaagatttg
    25921 aaggagagac tttaccatct ttaaaatcaa tggatacaaa aggattagta atattcttag
    25981 gaactttctc taaaatattc tgcccaggat acagattagg atggacttgt gcttctccag
    26041 aaattttatc taagtttaac tttgcaaaac aaggtgcaga cttacaagca tcaactatat
    26101 ctcaaatgga agtaagcaaa tttatggata tgtatgacct agatgcacat gttgacaaaa
    26161 taaaagcagt ttatgttaaa cgtagagatg taatgcttaa gactatggaa gaagagttcc
    26221 cagaaggatt agtatttact catccagaag gtggattatt tacatgggta gagcttccaa
    26281 gtaatttaaa tgctaaagaa ttaatgccaa aatgtttaga taagaatgtt gcttatgttg
    26341 ctggaggagg attcttccct aatggtggta gagaaaatac tttcagactt aactattcaa
    26401 atatgccaga agaaaaaata atagaaggta taaaaaatat agcagctgta ttaaaagaag
    26461 ctatgggtgt agaagcttaa ttaaaaataa taacttatat ataatacaag ggatggttgt
    26521 tgccatccct tgtttgtttg aaaagtatat aattcacctt atactaaact ttgtccaaaa
    26581 tttaaattga cagaaataag aataaatgat aaaatgtgta ggtatgagat tttagtctca
    26641 tttctctgct ctaagagtgt agagccgtta agtccaaagg gaggtgaatt atagtgagaa
    26701 attatgaatt agtttatgtg gtaaagccaa actctgatga agaagtaaga gaggctatac
    26761 taaacaaagt taaggaagtt gttgcaactg acggagaaat agttaaagtt gatacttggg
    26821 gaactaaaaa attagcttat ccaatagcta agtttacaga aggtttctat gtattagtta
    26881 acttcaaatc agcagtagac gttcctaaag agatagacag aaacttaaag ataaatgaaa
    26941 acgtaataag acacatgata gttgttgctt aataactggt tttattataa aaaataggtg
    27001 gtgtttttat gaatcaagtt gtattagtgg gaaggttgac tagagaccct gaattaagat
    27061 acatcccagg tacaggaaca ccagtggcat cctttacaat agccattgat agagactatg
    27121 taaaaaaaga tggatctaaa gaaactgatt ttatacctgt tgaagtaatg ggtaaatctg
    27181 ctgaattttg tgctaactac ataactaaag gtagattagt agcacttcaa ggatctataa
    27241 gagtagacaa ttatcagaca caatcaggtg aaaagagaac ttttacaaaa gtaagtacta
    27301 gatcagtaca agctttggat agtaagaata aatcggaaaa ttcatataga gagagtgctc
    27361 cagcttttga gccaagcttt gaacctcaag gtttagaccc acaaggtttc caagctatag
    27421 atgatgacga tataccattt taaaaaggag ggaaaaaagt catgatgaat aaaaaaagac
    27481 gtaagaaaaa aagagtttgt caattttgtg ctgacaaaaa cgctaaaata gattacaaaa
    27541 gtactcaaag attacaaaag tatataactg aaagaggaaa gatattacca agaagaatat
    27601 ctggaacttg tgctaagcat caaagagaat taacagttgc tataaaaaga gcgagaaata
    27661 tagcactttt accatataca ttagactaaa aaaatgagcc ttgtgctcat tttttattat
    27721 atgtatttat atatctaaat gaggcgagct ataaagctcg tttttttgtt tatatactta
    27781 aatatattat tatttatgat ataatattat atattaagga gggttgtaaa cgtgaaaata
    27841 gataaaggtt ctgagataac gagaaacata aagataatag aatggatgaa aacagaaata
    27901 ttaatgagtg taagtgattt gtttaattta ttgtttaaag gggtaaaacc attagatgaa
    27961 gcaattcaag atactttagc aaatataata atgataacat atctcttagc aaagagatta
    28021 ggaattagtt ttagagatgt ggattataaa gtcaaggaaa aaataaaaat tggtatagat
    28081 gaggaccata gcgttgaaag atggtatgga gatttatcta atttaaaaaa acatatggat
    28141 aatagggagt gatattaaat tgaataataa aattagacta tctaaggcaa tgtctataat
    28201 agttttagct ataataatcg ctttagtaat agcctatatt ccaatgctag gaatatttag
    28261 tatgttagca gctgttccat atgtaattat aggtgctatt actgatagaa agtattctct
    28321 tatatctatt ttggttacat tttgtgtact gattctattt gcagacattt cttatgcatt
    28381 aaatatatgt ataatgtatt ctttaccagg tatagtaata ggaaagatgc taaagagaag
    28441 ctttgaacaa gaggacagta ataaattcga accgatatat ggtggaacca taatatttgt
    28501 actttcaatg cttgtatatt tttttgtatt aaagacattt ttaaatgtga atctattaga
    28561 tgaagtttct aagatgatat cagaaattgt aaatattcaa aaaaatagtt tttctgcaac
    28621 tgaacttaaa gtgtttgata aaatgaagcc agatgagatt gtaagttatt ttacaaatat
    28681 gctaccaatg atgttatttt tacaagggtt gttatcagcg tttgtcacat attatttgag
    28741 tgtatttttt ataaaaagga ttacaaagat gaatataaga tttcctaagt ttgctgattt
    28801 ttatttaccg ggaaatgcta tactcacaac tttcttactt tatttattag tcttatttat
    28861 tcaaattatt ggttcaaaat tatatacaga attaataatg actaatttac agctagtttt
    28921 taatttaatg tttgtaattc aaggtatagc agtttgcatt tatttcttaa agaaatggat
    28981 aagacaggga ccaaataaaa ttatgttttt ttcagggata attttatgtt tatttggatt
    29041 tatgggaata tcttttgttg gtatggtaga tagtataatt gactttagaa aggtaagaag
    29101 ctataaatcc acttaggagg atagaaatga gtaataaaca aacctttaaa ttaaatatgc
    29161 cagaaataaa tttatatata attgttattg gtatttctag tataattttg ctttattaca
    29221 acttgtatgt agggtgttta tttttctgta tttttgtata tatggttttt cacaattgga
    29281 gaactactaa tattagacgt catgaatgga cagagtatat tcaaaattta tctttagata
    29341 tagatgaaac aactaaaaaa gccataataa atcttccaat accattatgt attttagaat
    29401 ttgatggaaa tatatcctgg tataatggaa agttttatga tatgattggt caaaaagatt
    29461 tacttgataa aaatatagag gatatagtta aaaatcttaa tttaagaaaa gtattaaatg
    29521 aaaataaaga gatgtacaca gaaataaact ataaagaaaa agaatataca ataatttata
    29581 atgttataaa aaatgatcaa gaaaaaaatc ctaaatattt gatgatatta tactggatag
    29641 acaagactga atatttaaaa gtaaaacaaa attatgatga tgaaaaaaat gcaatgatgc
    29701 taatacaagt agatggatat gatgaagtat taaaaagtgc agcagaagat aagagagctt
    29761 taattaatgt tgaagtagag aaaatacttt ctgctttaga attaaattca aatggtgcat
    29821 tgagaagaac ttcaaaagac aaattcttct tggtaatgca taaaaaagag ttaaaaaaat
    29881 tggaagctga gaagttttct attttagata caataagaca tattgattat ggaaataatc
    29941 ttcctgttac tataagtata ggaattggta tagatggaga tacattaaat gagaacctaa
    30001 aacttgctac gggtgctctc gatttagcac ttggtagagg tggtgaccag gctgttgtaa
    30061 aaacaaaaga taaatttgtg ttttatggag gaaaatctaa agctgtagaa aagaaaacca
    30121 aagtaaaatc cagattaata ggtcatgctt taagagaggt tatacaacaa agtgaccaag
    30181 tttatataat gggacataaa tatcctgata tggatgctat gggagcagca gttggtgttt
    30241 atgatatatg taaatcctgt aacaaaactg caaacatagt tttacaatct gtaaatgaat
    30301 ctatagaaat atttataaat aaaataaatg aaaataatta ctataaaaag ttgtttattg
    30361 gaaaagagga agctattgac aactgcacta aaaatacatt agttgtagta gttgatactc
    30421 atagaccaaa ttatactgaa tgtgaagaac tgttaaagct atcagagaag gtagtagtta
    30481 tagaccatca tagaagaggt gtagagttta taaatgatgc agtgctttta ttccatgaga
    30541 tatatgtatc ctcaacgtgt gaaatggtta cagagttagt tcagtatatg gatgaagatg
    30601 taacaataaa taaacttaca gctgaagggc ttttagcagg tataagtcta gatactaaaa
    30661 actttgcatt caaaacaggt gttagaactt ttgaagctgc ttcttacctt agaaaagttg
    30721 gagcagatac tatagaggta aagaaattct tcaattctga tgtaaaagat ttcataataa
    30781 aagctgaaat aattcaaagt actaaaataa ttaataatag aatatgttta gcatattcaa
    30841 gtactgagat agatagtata aatgttataa tagctcaaac tgctgatgag ttattaaata
    30901 tcaaagaagt agaagcttcc tttgtattag gtgaaaaaga tgatactata tttataagtg
    30961 cgaggtcttt aggacaaata aatgtacatg tacttatgga aaaattagga ggcgggggac
    31021 acatagatat agctggagca cagttaaaaa atgtttctct aaaagaagct tataagatgg
    31081 taaataaaat aatagaggag tatttggagg aggaagaata gatgaaagtt atattattaa
    31141 aagacgtaaa aggaactggt aaaaagggag aaatgaaaga agtaagtgat ggatatgcaa
    31201 gaaatttctt atttcctaaa aagatggctg tgcaagcaga tagtgtagca ataaaagaat
    31261 taaatgaaaa gaataaatct aaagaaataa aagcacaaaa agagtatgaa gaagcagttt
    31321 tattaggtaa gcaaatggaa gaaattaata tagaaatata ttcaaaagca ggagaaggtg
    31381 gaagattatt tggttcaata acttctaaag aaattgctga gcaattaaag aaacaaaaag
    31441 acatagatgt agataaaaga aaaatattat tagatgaacc aataagatct ttaggttcaa
    31501 ctttcgtaga aataaaaata catcaaaaag taactactaa aataagagtg gatgtaaaag
    31561 aaaaacagta ataactagag gtgattaaga tggaagatat gacgagaatt cctcctcata
    31621 gtgtagaatc agaacaatca atattgggtt ctatacttct agataaagat gctataataa
    31681 cggttacaga aactataaag cctgatgatt tctataaaga ggctcacaaa ataatatatg
    31741 aatgtatgat aactttaagt aataaaggtg agcctataga cttgataaca ttaacagaag
    31801 aactaagaaa acaagggcat cttaatgata ttggaggaat cagttatatt acaagtcttt
    31861 ctactatagt accgacgact tcaaatgtaa aatactatgc tgatatagta aaagaaaagt
    31921 ctgtacttag aaagcttata aaagcttcta atgaaataat aaatctagga tatagtggag
    31981 caactaagat tgaagatgtt ttagaacaag ctgaaaagag tatatttgat atatcacagg
    32041 agaaaactag tgatgatttt aaatctataa acttagtttt gatggatgcc tatgatatga
    32101 tagaaaaatt atacactaac aagagtgatg tcactggtat aactactggt tttaaagatt
    32161 taaacaaaaa aataaatggt cttcaaagga ctgatttaat attaattgca gctagaccag
    32221 caatgggtaa aactgccttt tctttaaact tggttcaaaa tgcagcctta aaaggtgatg
    32281 cctctgtagc tgtatttagt ttagagatgt ctaaggaaca attagtacaa cgtatgttat
    32341 cttcacaatc tagtgtagaa ttaaaaaaaa taaagactgg aacacttaat gataatgatt
    32401 ggcctagaat tatagatgct atggcagtat tatcagatgc caaaatacac atagatgata
    32461 ctcctggtat aaaaatatca gaactaaggt caaagtgtag aaagttaaaa atagaaaaag
    32521 gactagattt agtgcttatt gattatcttc aacttatgga aggtgaaggt aataatgaaa
    32581 gcagacaaca agagatatct aagatatcaa ggtcactaaa aatattagca aaagaattaa
    32641 actgtcctgt agtagcacta tctcagttat cacgtgcacc agaacaaaga gcagaccata
    32701 gacctatgtt atctgattta agagagtctg gagcaataga acaggatgca gatatagtta
    32761 tgttcttata cagggacgaa tattatcatg ctgattctga gagtaaaaat ataggagaag
    32821 taataatagc aaaaaataga cacggtgaaa ctggttctgt ggaacttgtt tggcttggtg
    32881 aagttcaaag atttggggat aagttgagag acctataatc taacgaatat ccacagaacg
    32941 tcctttcttt ctacaggaaa aatgtggata catgtcacta aatatgtcca aacattgaaa
    33001 tttagctaaa aaactatcca cactatccac agatggcctg ttgataagtg tgaataagta
    33061 gataaagcaa gcttttggct tgctttatct gtgtataaat attaattatt aggaggaata
    33121 atatggaaaa aataagctta aaatatatat atcccaatat aattaaagta cttgatgaaa
    33181 taaacttatt tagagtaatt gacaataatt taagagaatc tattgttgtt tatgctaata
    33241 atgtggataa tcagtatcat ataaacatga ctaatacaaa ctttggaaat ataattaaca
    33301 tatgtaaatt agaaaaactt ttagatgtgg ataaatttat ggaaaaagtt ataaaatatg
    33361 aaaaagaaat aatagagaaa gaagagtttt caaaaataga agaatatatg ttaaatattg
    33421 gagaatacta aaaaagttga aaaaaatacg aatgtttgat agaataagta agtaaagtgt
    33481 tcgagagtat ataaaatata gaggtgaatt acatgaaaac agttgcaatt gttggttctc
    33541 aatggggaga tgaaggtaaa ggtaaagtta tagattatct tgctactcaa gcagatgtag
    33601 tagtaagagg gcaaggtgga aataatgcag gacatacatt agttgtagaa ggtaaaaaat
    33661 acgcattaca tctaattcca tctggagttt taaatccaaa tacagtgaat ataatcggaa
    33721 atggaatagt gtttgaccct aaaggatttt tagaagaatt ggaaatgttt aaaacggata
    33781 atataagtac agaaaatata aaaataagtg atagagcaca tgtcatattc ccatatcata
    33841 aagaactaga tgcattgtca gaagaagcca gaggcgattt aaagataggt acaacaaaaa
    33901 aaggtattgg accttgttat atggataaaa cagagagatc tggtattaga atatgtgact
    33961 taatggataa agataaattt gctataaaat taaaagctca aatagatgct aaaaatgaaa
    34021 tagttaaaaa tatatatgga aaagaagaat tatttgattt tgaaactata tataacgaat
    34081 atctaggata tgcagagcaa ataagaaaat atgtggcaga tacgtctgtt attgtatatg
    34141 atgcagtaag agcaggtaaa aaagtattgt ttgaaggagc acaagggact ttattagatt
    34201 tagatttagg aacttatcca tttgttacat cttctcatcc aacatctggt ggatttgcaa
    34261 ttggtgcagg tatagggcca aatatgataa aagacgttgt tggtatagta aaagcatata
    34321 ctacaagagt tggagaagga ccgtttgtta cagagcaaat aaatgaaact ggagataaga
    34381 taagagaaca aggacatgaa tttggggtaa ctactggtag accaagaaga tgtgggtggt
    34441 ttgatgcagt tatagttaag tatgcagcta gagttaatgg attgactagt atttcattta
    34501 tgttacttga tgtattaact ggatttgata agataaaagt ttgtacgtct tataaaatgg
    34561 gcgataagat aataacggat ttcccagctt cattagatga tttagcaaaa tgtgaacctg
    34621 tatatgaaga attagatgga tggaatgaag atataactca aatagataac tttgatgacc
    34681 ttccagaaaa tgcaaagaaa tatgtagcaa aaatagaaga actagttgga gtaagtgtag
    34741 atatggtttc tgttggtcca aatagagctc aaactataat aagaagaaat atatttgctt
    34801 aatattttat caattaaata atttaaaata gttgaagaag agtatctcaa gataatgttt
    34861 atagttatta gagatgctct tttatatttg acttacattg attttttatt aagttaaagc
    34921 tacttataca tattctaact gaaattaaag ttgaatttat gtagatacat ataggttttt
    34981 taaaaagaat tttgttgaaa ataaatggct aaagatgatt ttaattatat tctaaaaaaa
    35041 gtaaaaaaaa ctattgacta aaaattatat agacgctata atataacttg tgagtgaaag
    35101 aaacgacgaa aaaagtcgaa aaaatgtggt ctattagctc agtcggtaga gcacctgact
    35161 tttaatcagg gtgtcccgcg ttcgagtcgc ggatagatca ccaaaatatg gctcgttggt
    35221 caagaggtta agacaccgcc ctttcacggc ggtaacaggg gttcaattcc cctacgagtc
    35281 accagtatgg gactatagct cagctgggag agcacctgcc ttacaagcag ggggtcacag
    35341 gttcgagccc tgttagtccc accaaatttt gaattagaga taaatactag attatatgct
    35401 ggtgtggctc aacggtagag cagctgactt gtaatcagca ggttgtaggt tcgattccta
    35461 tcaccagctc cacaaagtac ataaattcaa attaatataa ttttctatgt tacaggatac
    35521 ttttaagtat ctttttttaa tttcattttt attgtattaa aaatgaaatt aaacatacaa
    35581 caaaattttg aattacttgc aatgtgatag ttttaaatat aaaatttaga atcattctag
    35641 cataatatta tgtaatatag caaatttaat ttatagtatc aataaatata gactactaag
    35701 aatgtaaatg atttgttaga ttaaggtaca ttagatatgt aaatagaaaa ataaaaagta
    35761 tgttttaatt tatgtgatat aagttttaaa cagagaagca agtttgctct tacctctcta
    35821 tttaaaaata aaaaaatgtt ttttaagggc tattttattt aaatctttcc catctcaaat
    35881 catctccagt aaattctaaa atcctaaact tatctaatat tctagagaga gttctttgag
    35941 tgtaagtatt tcctatttga gatggtgtta agttggttga aataatgatt tttttaccag
    36001 caacaagtct tgtatttaca atattgaaga tttctccact agtaaaagaa ttattaagtt
    36061 cagttccaag gtcatctatt attaataaat cacaatcaaa caagttttta taattatctt
    36121 cacttatttg attatttgtg tctcttctaa atttataatc ttcaagaata tctaaaattc
    36181 taaaagaagt ttgatagatt acaacattgc ctttatctaa caattccttt gcaatacaat
    36241 tgcacatata ggtctttcct agtccagtag aaccataaaa cagtagattt tcactattat
    36301 ccttcttaaa atcatgaaca aaattttcgc agatacttaa attatttagc atattttctc
    36361 taggagatat ttcaccatca gagcctttct taggagaaaa tatattcaag ttaaaattcg
    36421 aaaaattttc ctgacttaaa atcctatcta aattagacat tttataagct tcatttacaa
    36481 tttcttgttt gaggcaagaa catttttctc cattaggtaa aaatccttta tctttacaag
    36541 aaatacattg aaactttatt tccaagtaat caagaggtat gttactttca gctaaaagtc
    36601 tttctttctt tacttttaaa gattctatat tctctttagt cttctttaca atttcatctt
    36661 tagacttagg atttaaaagt actatcttag ctaaactgag acctatttta gaaatttcat
    36721 catctataga ttttatctca gggattctat tatatacctc attttttcta tgttctaata
    36781 agagttcatt atcatctctt ctcttagcat atttagcaag tatttttctt attttatcct
    36841 cattcattta atcacctact taaacttttc tttttgactt tttttaataa tctcatcgag
    36901 ctcatcagag gtatactgag tgaatgtttc gttgaaatta tgaaacttag tttttttgta
    36961 agtattgttg ttttgagtag tattaatctg cttcttatta atattctcct taactatacg
    37021 ttcttcctct ttttgcttta aatcatttaa attttttata tttttttcat tccaattttt
    37081 aattatacca tttatataag atatacttgg gtttgaaata ttcttagact tagaacatgc
    37141 atttattatc aaatctatat ccatattaaa tttatcaaac caggtgtcca taagttcttt
    37201 ttctgatttt gatggttgtc ttgaaaatcc caattcattg aaaatcgttt tatataaaat
    37261 atatcgttca cttcttacca aaaagctttc ttcaacatct tttggcgtat ataaatttga
    37321 atcataccaa tttctaataa tgccttcaat gtatttaact ggctttgatg taccagtttt
    37381 atctttgacg tattcatatg cgcaaagtat catatcgggg ctcatattgt atttattcat
    37441 tatatccata atactcatct tttcacttgg gtcgagatat cgcccaacta ttttatttat
    37501 agaattaaac atttttctaa tacttggatt ttcagaagta gatacaactt tatctgtatt
    37561 agattttata gatgaattat tgcttaatat attctcaata tagaaatttt ttaaatctaa
    37621 aaattctatt gaataattaa aattatcata ttcttcattg tcatgtattt ttataatctt
    37681 cttttcttcc cagaatttcc aagcagataa cacatcagac aatggtatat ttaggttttt
    37741 ggctattgaa ttattgtcga acttaggatt agaagtaata tcacatgctt gtctatatcc
    37801 taaaagataa acttttacgt ataacccgtc cgccattggc ataaatatat ctataaagat
    37861 attgggaata gtgatttcac ctaaatctat ttcattgctt tctttaaaaa acattttatc
    37921 acctcagatt aaattatatc attttttata ttatatttgt ctgtaaacaa ataaaaggtc
    37981 acaagattag catgtaaata ttttctaaaa gtattcattt tgaaaaggag gagatatttt
    38041 ggttataaaa ttcaaccaaa aagtaagaag aacattatca gtagcgattc tatttatggt
    38101 attaataata ggttgcttta tagaaaagga tgacagttta gaaacataca aagaaaaaac
    38161 taattcaaaa ataaatcagt ataatatgga tgttatattt gatgatgaga ctaagaggtt
    38221 aatgtgcaat cagaatgttg actacataaa taatacaaaa tcaaacatag ataaaatata
    38281 tttccatata tatccgaatg ctttttccaa gaaagatttt gctccttttg aaaaaagtga
    38341 aatgacaagg gcttatccta atggatttaa cgaaggatat atagatataa agaatatatt
    38401 aaataataat agtaaaatgg agtataaaat taaaggagac aaaaatgaca tattagaaat
    38461 agaattaggg aaagagttaa aaccaaatga aagaatgtca ttagatataa aatataacgt
    38521 aaaaatacca aattcaattg gaagatttgg atatggagaa aatactataa atgttactaa
    38581 ttggtttcct atagcttgtg taaatgatga aaggggatgg aatttaaaga gttatgaaac
    38641 tattggcgac ccattttata gtgagactag cgattttcat gtgaaactat tgattccaaa
    38701 taaatataaa attgctcata caggaaaact tattgatgat aaacatgata ataagaaaat
    38761 gctatatgaa atagaagctg aaaacgttag agattttgca tttatactaa gtagtaaatt
    38821 tgatgtaaac aaagtagatt caaagggagt agctatcaat acatataatt taaataaaaa
    38881 gctttctaaa aaagcaactg aagtggcaaa agattcaata aatatattta gtgaattatt
    38941 tggaaagtat ccttacaaag aattttctgt tgtagcaagt gactttttta taggggggat
    39001 ggaataccct atgcttgtta tgatagacca aagtttatat aatgaaaaaa atgaattttt
    39061 actagaatat gttattgccc atgagacagc acatcaatgg tggtattctg ctgttggaaa
    39121 tgatgaaata agtgaaccct ggttagatga agcacttact gaatattcta caatagttta
    39181 ttttgaacaa aagtatggta aagatatggc aaacaagctt ataaaaacta tggagataca
    39241 gacaaaaagt tatttgagtg aaaatatatt taagccagca aatgagtata aaaattctac
    39301 tgaatacagc ttgaatgttt atacaaaagg cgcaatagca tttaatgaaa taagaaaaga
    39361 agttggagat aaagtattct ttgagacttt aaatgagtac tataataaat ataagcataa
    39421 aaatgctaat ggaagagctt ttgtggaact atggaatagt aaaggtgtag atataaataa
    39481 gattataagt gaatgtaagt aaatagtgaa tgtaagtaaa ttttgtgcag agtattaaat
    39541 tttatttaat actctgtttt tttggtataa tataatttat aaggcagaaa aagaggtgaa
    39601 ggaatgctaa agtattgttc agtagggagt ggtagtagtg gtaattgcca ctatataggg
    39661 tataaaaata ctaatatact agtagatgca ggattaagtg gcaagagaat aactacaggt
    39721 cttaaagaca ttggtgtaga tgcagataaa ttaaaaggaa tatttataac acatgagcat
    39781 gttgaccata taaaaggagc aggtatatta tcaagaaagt ttgatatacc agtatttgct
    39841 aatataaaaa catggtgttc tatgaaggac aaattaggtg acatcaaaga taaaaatatg
    39901 aaagtatttg aaaatgataa aacctactca ttaggtgata tcatagtaag acctttttca
    39961 atagaacatg atgcgtctga cccagttgga tataattttt atacagagag tgatgaaaaa
    40021 atgtctatag ctactgatat aggatgtata actcaaaata taaaaaaaca tctatataaa
    40081 tctaaattag tagtattgga atcaaattat gaccctaata tgcttatgat gggttcatat
    40141 acatatgctt taaagaaaag agtaatgtca agtactggtc atttatccaa tgaagatgca
    40201 gctaatttct gcgttgaact tattaatgaa ggtacagaat ctatattatt agcacattta
    40261 agcaaagaaa ataattttcc tgagttagcg tatgagactt caaaaggagt attagcatca
    40321 aatgatatag tagttggaca agatgtaaaa ctagatgtac tatcacgaaa tgatgtttct
    40381 aatgtttatg agatgaagta gaggtgaata actttgctaa agagaaattt tgaaaatgtt
    40441 gaggataata aaattttata ctctataaag cagggtatga ttttagcgat acctgctatt
    40501 atgactggtt caactgctct tgtaattttg aatcttccaa taaaggctta tcaagaatat
    40561 ttatcaggct tgtttaatgg agaggttaca aatattctta actttataaa tgattctact
    40621 ttaggaatta tttcattaat aatattatta acaataagct atagttatgg aaaaatatat
    40681 gggagcaagt atacagtact tgttcctata gttgcaatgt gctctttttt agtattttca
    40741 catggtaatg agcttaattc ttatatagaa atatttaaaa caaagtggtt atttacatct
    40801 ataattgttt ctatgacatc ttctgtattg tttgtaaaac ttacagaatc ttttgttaca
    40861 agtgtaaagt ttcatacaga aggggctgat gcagacttta atatggtagt ttcggctata
    40921 gttcctttca taatagtagt atttttgttt tctattttta gagtggtaat gataagtcta
    40981 attggcagtt caaactttca agatatattt tataatttat tttcaaatgt ttttaataaa
    41041 atgggaacaa accttatgag tgctctgttg tttatttttt taatgcactt tatgtggttt
    41101 tttgggattc atggtagtaa tgtattagat accgtcgcaa aaaatttatt tgaaaatagt
    41161 atggcaatta acataaattt agttaataat aatcaattac caacagaaat ttttactaag
    41221 accttttttg atacaatggt tttattggga ggatgtggct ctttactatg tttagtaatt
    41281 gcaatttttc ttagtgaaaa acgtataaat gtaagaaagt tagctaaaat tgcttcaata
    41341 ccagctcttt ttaatatcaa tgagatgcta gttttcggga taccaattgt ttttaactat
    41401 attatgtttg taccttttgt aattactcct ataatcttaa ccgttacaag ttatgtggct
    41461 atgtctacag gtattgtacc atgtacagtt tcagctgtag aatggacctc acctatattt
    41521 ttaagtggat atatgtctac aggttcaata agaggtagtt tgttgcaagt atttaattta
    41581 tgtgttggtg ttatgattta tattccgttt ataaaaatgt cgcaaaatag atatacatat
    41641 atgtttaaaa ataatataga aaatttaaca aacttattaa aaaggtatga attaacaggt
    41701 gaacagccta atcttctaaa ccataatggt aaaataggtt caatttcaaa aatgttggca
    41761 tcagacttga agtttgctat gaaaagagga gaaatagagc ttttttatca accacaagtc
    41821 aattataatg gaaatgttgt gggagcagag ggattactta gatggaaaca ttcaatagga
    41881 gggttcattt atccaccact agttataatt ttggctaaag aagaagggtt cttagatgag
    41941 cttggagagt atattattaa taaagcatgt attgatttaa agaagagtga aaagctattg
    42001 agaaatcctg ttaagttctc tgtaaatata tctccagacc aacttgatga ccctaagtta
    42061 ccagagcaaa taaaaaatat cattagtagt aatgatataa atcctaatat gttaggtata
    42121 gaaataacag agcaaattgc attatcaggt tcacaaataa ttattgaacg tatcaatgcg
    42181 atacataact tgggaataaa gttaattatg gatgattttg ggatggggca tagctctctt
    42241 atatatcttc aaaataataa ctttgatata gttaaattgg atggttcatt agttaaagag
    42301 atacttacaa ataaaagaag tagtgaaatt ataatgacta ttgtaaatct ttcaaaaaat
    42361 ttagattttg atatagttgt tgagtatgtt gaaaatatag accaaaggga taagttatat
    42421 ggtttaggat gtgaaatata tcaaggatat tattatagca aagcaatacc ttttgaagag
    42481 tttgtagaat atgcaaatca agaaagagag atataatttt ataagagctt gtaaattcta
    42541 gtaatgcata gatttgtaat aaaattaagt ttaggagaaa tatatgaata taagtatagt
    42601 agtagtaggt aaaattaaag aaaaatattt aaaattaggt attgatgagt ttaagaaaag
    42661 gctttctaag tattgtaagt tagaaattat agagttggat gatgaaaaag ctccagagaa
    42721 tttgagtgtt aaggaaatgg aaattataaa agacaaagaa ggaaagaaaa ttttagggaa
    42781 gataaagcat aatagttatg ttatagcctt ggctattgat ggtaagggtt tatcctctga
    42841 agagcttgct aagactatga gtgatttagc agttagaggt aatagtagtc tctgttttat
    42901 aataggaggt tctttagggc tttctgatga ggttttagga agggctgatt ataggttatc
    42961 cttttctaag atgacttttc ctcatcagat gatgagattg atattgttag aacagattta
    43021 tagggcttat aggataaata atggagaacc gtatcataaa taaatgatac ggtttttgtt
    43081 aatttgacac ttaataatat taaaataaca aaaaagtatt gaaagtatat tgcatttaag
    43141 gtagaattaa ataaaatatt ataacattat aatgtttata tggttgttaa taaaaatttg
    43201 gaggaaaata tatgataaaa ataatgttgg cctgttcagc aggaatgtct acaagtttat
    43261 tagttactaa aatggaaagt gcagctaaag aaaatggtat agagtcgcaa atttgggcaa
    43321 taccagaatc aactatacaa aatgaaatag aaaaatgtga tgtattatta ctaggaccac
    43381 aagttagata tgtattacct aaggctcaag aaattgctaa gccatataat ataccagtag
    43441 aagttataaa tatgatgcac tatggaacag ttaatggaga agctgtttta aatagagcga
    43501 tagaacttaa caacactaag taatataaat tataatgtat ttttaatata taaattggga
    43561 gaatcaaagg atggatgaaa agactataga aatatctttt aatataatag gttatgcagg
    43621 tgaaggtaaa ggattagctt ttgaagcaat aaaagaggct aagaatggaa atatagaaaa
    43681 agcaaaagaa ttattaaaag agtctaggga agttataaat aaagcacata gatatcaaac
    43741 agaattaata caaaacgaag ctagtggaaa caggactgaa ataagtgtca tacttataca
    43801 tgcacaggac cacttaatga atggcatgaa ttttcaacag ttagctgagg aaatcgttga
    43861 tttacatttg aaattacaag ataaataaaa attaaagata ataaattaag agagttctta
    43921 aaagatacat tatgtgtagc acactaattg ttagattatt gattcaatat ttagtgagta
    43981 atacattagg attattttga ggactctttt tatatttagt ttaaagttaa taaatattta
    44041 ttattaataa tataagatta tatgttataa tattataaca tataatttaa gaggtgaatc
    44101 atgttaagaa atcaaccatt atatatacaa gttgtagata tattaaagca aaaaatatta
    44161 aataacgaat ataaaattga tgaaaatatt ccaacagaaa gggagttaga gcaagagttt
    44221 ggagtcagta aaattactat tagaaaagct attgagatat tagaatcaga aggatatgta
    44281 gagaaaaaaa gtggaaaagg gacaaaagta ataagtaata gcctttttaa taagctatca
    44341 aaagcagagt ctttttcaag tatactttta tctaaaggtc ataagttatc taaagagact
    44401 atttcaattg aaaaaataat aaatgataca aattcacaag tatttagtat atttggagaa
    44461 aaatgtagca agattactag aatgtattat ttagatagta agccatatat attttttact
    44521 cattatattc cttttgatga aaatctaact gttgaaagtt ttaataatga aagttcttta
    44581 tatatgtact tatataggag aaatataaat ataactagat ttgaagatga attttttata
    44641 gaagaatcag aactagaggt atcaaaggct ttaaatctag ctaaagacat attattagga
    44701 agaaaaagaa agacctatga tgagaatgat aatatagttg agctatcatt tgcaagatat
    44761 aatacagaag tacaaaacta tgtaattaaa tacgaaatat aataagaatc ctgagtttat
    44821 tgaaatgata atatgctcag gatttttgtt tagttgtaaa aataaaaaat attataacat
    44881 tataatgttt acaaccaaac attataatgt tataatattt ttaataaaca agtgatatta
    44941 tcaattacat aaaaattagt ttatgttatg aaatcgttat tgcattataa ataaaggata
    45001 atatcaataa tacaggggga taactatgga aaaattcgca agttttttag agcgcaagat
    45061 tatgcctgta gcagcgaaga taagtaatca gagacatatg agagctgtaa gagcaggtat
    45121 aatagcaaca ttaccactaa caatagtggg gtcatttttc acaatattat taaacatacc
    45181 tattgatgct tatatggatt ttatagctcc atttaaagca atattagata taccatttag
    45241 atatactgta ggatttttat ctctatatgc tacttttggg atagcatcat cacttgctaa
    45301 aagctacgat ttagatacaa caggtgttgg tatgcttgca gttatggcat ttttagtttc
    45361 tacaatagtt ccaactcaag tagttgaacc agtaggtgga gttatagaag ctggtagatg
    45421 gatggatatg gcaaaactta attcgccatc attatttgga gctatcgtaa catcattaat
    45481 tacagttgaa atatatcgtt ttatgaagga aaagaatatt acaattaaga tgccagcagg
    45541 agttccagta gaggtttcaa attcatttac tgcattatta ccgacagcag taattttaat
    45601 gttattctgg gtaataagac atattctagg atttgatata agtgttgcac taagcgctat
    45661 gttatcacct ttaaaaggat tcttatcagg aaatagttta ggtggtgggt tactgacagt
    45721 attcctaata actttattct gggtacttgg aatacatggt ccagctataa tgggaccagt
    45781 aataagacct atatgggaca tgtctatagc agaaaatata actgcattca cagaaggagt
    45841 atcagctcat gcaatgccaa acatatttac agaacaattt ttacagtggt atgtatggat
    45901 agggggagca ggggctactc ttgcattagt tgtaatgttt atgttttcta agtcacaata
    45961 tctaaaaagt ttaggtagat taggattttt accagggtta ttcaacataa atgaacctat
    46021 aatatttgga gcaccaatag ttatgaatcc aatactaggg ataccattta taattggacc
    46081 tataataatg acaattctat catatgttct tactattaca ggggttatac ctatgatggt
    46141 agcaagatta ccatttgcaa tgccagcacc aatagcagca gtaatgagta ctaactggag
    46201 tatactagca ggtatcttag tattaatcaa ctttgtgata tcatttgctg tttattatcc
    46261 attcttcaaa gtttttgaaa aacaacaatt acaaagagaa caagaagaaa tgagtgaaaa
    46321 tttataaaaa tacatagata tagaggtttt gtactatata aaatatagaa aaagcttata
    46381 agttattaag ggtgaaggta ggaggaatga cctttgcctt taattttata tattttaaat
    46441 aatgtaattt gaggaggaga aagatggaaa gaagattagg aatatcagta tatccagagc
    46501 atagtaacat ggaagatgat aaggcatata taaaaatggc ttcaaaatac ggatttaaaa
    46561 gaatatttat gtgcatgctt tcaattacaa gacctaagga agaagttaaa aaacatttta
    46621 aagagattat aacctatgca agagaattag gatttgaagt aatccttgat atagctccaa
    46681 acatatttga tatattacaa atatcttatg atgatttaac attttttagt gagttaaatg
    46741 ctagtggaat aaggcttgac tatggatatg atggaagtaa agaagctatg atttctttca
    46801 atccatatga tttaaaagtg gaactaaata tgagtaatga tgtagcatac ttggataata
    46861 taattactta caagccaaat gtaaataata tatatggttg tcacaatttt tatcctcaaa
    46921 ggggtacagg cttgccatat gatttttttg taagatgttc aaatagattt aaaaagtatg
    46981 gaataagaac tgctgctttt gttaattcaa aatacgcaac aatagggcct tggaatataa
    47041 atgatgggct gtgtacgctt gaaatgcata gaaacttaga tattgctaca gcaaccaagc
    47101 acttattcgc aacaggattg attgatgatg taattgtagg gaatgcatat gcaagtgaag
    47161 atgaattaaa aagaatgtct gaagtaaata aatatcaagt tgtatttaat gtagtaactg
    47221 aagaaaaaac ttctgaagta gaaaagacaa taatggcaaa tgagcaacac tttagaagag
    47281 gggatataac caaccaagta gcaaggtcta cagaagtaag aaaaaaatat gctaaagaaa
    47341 atttccctaa gcatgataat acgaccatct ttaaaagagg agatgtagtg attggtaatg
    47401 atgactttgg aaagtataaa ggggaattac agttgatact tcaagattgt gcagatgaaa
    47461 gaaaaaataa ggtgggtaga ataaaggaag acgaaataat acttttagac tttattgaac
    47521 cttggagtaa atttaaactt attttagagg gagagtaaag tgaagataga catattaata
    47581 aaatgtggaa aaacagtaga taaaagtata attgatatag ctatattaga cgataaaata
    47641 atagaagtaa aaaatcagat agatgattct aagtatcagg caaatcaaat tataaaccta
    47701 gatggagaaa aatttattag tgctggttgg atagatatac atacgcactg ctacgaaaga
    47761 ctagaccttt ataaggatta tccagatgag gttggaataa aaagcggtgt tactactgta
    47821 gtagatgcag gaacaacagg agctttagat ataggtaaat tttatgaaga tactaaaaaa
    47881 tataaaacaa atgtatatgc tttaataaat attgctaagc agggaataac atctcaagat
    47941 gaactttcaa gtatgatgaa tattgatgaa tatgaactta gaagagcagt taaaaaatac
    48001 aaagattttg ttgttggaat caaagctaga atgagtaaaa gtgttgtaat ttcaaatgat
    48061 gttgaacctt taaaagtagc taaaagaata aaaaatgaac ttaatctacc tatgatggtt
    48121 cactttggga gttctcctcc aacgatagag gatatatttg actatatgga aaaaggagat
    48181 atattaaccc atatatataa tggaaagcca aatggaatac ttagaggcaa tgaggttaaa
    48241 aaagaaatca tagaagctag agaaagaggt ataatccttg atgtaggtca tggaaccgaa
    48301 agttttagta tggatattgc aatgaaatct aaggatgcag gaatttttcc agacactata
    48361 agtacagata tatatattaa aaatagaata aatggtccag tgtataatct atctacaaca
    48421 atggaaaagt ttatatatat gggctattca cttgaagata taatagataa agttactaaa
    48481 aatgcagcag atgcaataag tttaaaaaat aaaggtttaa taaaagaagg atatgatgct
    48541 gatttaacta tatttgatgt ggttaatgaa gaaaaagaac ttcaagattc tttgaataag
    48601 tcagtaatca caagtacgtc tataaagcca agagcagtag tagtcaatgg agaatattta
    48661 aatataggta agtctatagg agagaatgag tagaagggag aaactattgt atgagtagta
    48721 tatttgaaaa atactctgta agacgtgtta taaatgcttc aggcaaaatg actatactag
    48781 gagtatcaac agtagataag gaagttattg aagcaatagg tgaagggtgt aaaaatttct
    48841 ttataataga agaacttata aataaaacag gtgaatatat agcaaattta ttagggtgtg
    48901 aaagtgcact aatagtttct agtgcatctg ctgggattgc acagagtgtg ggagcaatga
    48961 tagcaaaaga tgatatgagt ctagtttaca atgtaaataa tcctaagaaa caagttaaaa
    49021 gggaaataat aattccaaag ggacataatg ttgattatgg agttccagta gaagtaatga
    49081 taagacttgg aggaggagaa gttgttgagg ccggttatgc taatatgtgt aacaaggaac
    49141 atataataag agagataaat gaaaatacag cagcaattat gtatattaaa tctcatcatt
    49201 gtgttcaaaa gagtatgcta agtgtattag atgcagtaca agttgctaaa gaatataatt
    49261 tacctttgat agtagatgca gcagcagaag aagatttaaa tgtatattat gaattgggag
    49321 cagacctagt gatatacagt ggtgctaagg ctatagaggg tcctagctca gggttagtaa
    49381 taggtaagaa aaaatacata gataatgtaa agcttcaatc aaagggtata ggaagagcta
    49441 tgaaaatagg aaaagaaaat attgtaggtc taacatgtgc aattgagaga tatataagta
    49501 atgataaagt aactttaaaa gaaatggagg ataagctaaa tccatttata gacaagataa
    49561 atactataaa gggagtgagt gctagtataa ctagggattc agcaggtaga gaaatacttc
    49621 gtggagaaat tgactttaat gaagatataa taaataagtc aacacaacaa attataaatg
    49681 aacttagaag tggagagata gctatataca ctagagacta taaggctaat gaaggcaaga
    49741 ttgagattga tataagaagt gtaactggaa gtgatttaga tactatttat aataggataa
    49801 aaaatatagt agaaagttag agtgtagagt aagtgacatt ataatgattt aataaattaa
    49861 attttaccaa tggaggggat aaatatgaat tgtataccac aatattataa aaatagagtt
    49921 tgtttaaatg ttcttgcagg aagcataaaa aatgctaaag aagtatatga tgcttgtgac
    49981 gggcatgtat tgataggaat actatcaaag aattatagca ctgtagaagc agctattgaa
    50041 gatatgaaaa aatactcaaa ggaaatagat aatgcaatat ctgtggggct tggagctgga
    50101 gacccaaatc agtcaaatat ggtatctcag atttctaaag aacttcagcc acaacatata
    50161 aatcaagtgt ttacaggtgt ggctacgtct agagcattgc ttggtcaaaa tgatagtata
    50221 ataaatggat tagtttctcc aactggtaaa ccaggatttg taaaaatatc cacaggtcca
    50281 ttaagttcaa aagaaaaaga tggtatagtt cctatagaaa cagcaatagc tatgatgaaa
    50341 gatatgggag gaagctcaat taagtttttt ccaatgggag ggcttaaaac aaaggatgaa
    50401 tatatagaag ttgcaaaagc ttgtgcaaag cataattttt atttagaacc aacagggggt
    50461 atagacttag ataacttcaa agagatagtt caaattgcac ttgatgctgg tgtagaaaaa
    50521 gttatacctc atgtttatac atcaataatt gataaagaaa caggtgaaac gaaagtggaa
    50581 gatataggag tactttataa aataatgaaa gaattattgg gatagattta tgaatatatt
    50641 agcttttggt gaaataatga tgagactttc agtacctgac tacaagttct taacacaaac
    50701 caatgaacta aattatataa ctacaggcac gggattaaat atattatctg gacttaaaaa
    50761 ctttggatac aatacctata tgttgacaaa gttgcctaat aataatgtag gtaaagcatc
    50821 aagtgcaaat ataagaaagc ttggagtaaa agatgacttt atcacgtatg gagggaatca
    50881 tataggagtg tattttcttg aaaatgggta tggagaaaga ccttctgaag tgacttattt
    50941 gaataggtta aatagttcat tttgtgagag taaattacaa gattatgata ttgataaatg
    51001 tttagaagaa atggatatag tccatatttg tggtattgct atgcaactta cagattcagt
    51061 taaagagatt gctttagaac tagctagaaa atcttatgaa aaggggataa aagtattctt
    51121 tgattttaat tttagacaat cattaaatcc agaaaggact tatgatgatt tagtaaatga
    51181 ttataagaaa atactaccat attgtcatgg gctgtttgct agttatagag atataaataa
    51241 gatacttaat atagatagtc taaatataaa atctagttca gaattgatta aaaaagagta
    51301 tgatatagat attgtagctg gaactatgag aagatttgat gatatgaata atagatttat
    51361 aaagggatat atatcaaatg atgatggtta ttttgaaagt agggagtata agctagaaat
    51421 atatgacagg gttggtgccg gagatggata tgtagcagga attatatatt catatttgga
    51481 aaatatgaag agtgaagata tagttgagtt tggggttgct agttcggtgt tagctcatac
    51541 aacttatggg gataattctt tagtaagcac aggtcatgta cttagactga tgaataatga
    51601 aaaaatagat atattgagat aatacatttg tatataataa aatttttatc ttttttagac
    51661 acatttttga cttgcttata tttgttaaac agagttattt ttaatgatta taaatattaa
    51721 attaaaagtt aaataataaa atagtagaaa atagcattaa ctatagaaaa aatggttaat
    51781 gctattttta tcgttaatat tgattaattt tatattatag aaatgaactt ttctaatgct
    51841 ggatttgtat tttttttatt ccatacaact acaacttcaa ttctattttc tacttctttt
    51901 ataggtacta ttgcaatatc acgttctatc attgtatctg taacacaaaa tgaaggtaaa
    51961 atggatattc ccatgttaga agataccaat gattcaatag tattaagatt atttgaacgt
    52021 tgtaccacat taggtacaaa ccctgcttca cgatatctat tctgtatcat tgacttttgt
    52081 ccatactcat cccctgtttc ctttacaaaa ataaatttat catgttgtaa ttcaattaaa
    52141 tcgatagaat cctcattgta aagtggatga ccaggaggta aaagagctac caaagaatat
    52201 ctttctacag ttttatattc aaaatctttt aatggttgat tttctggatt aataacaaaa
    52261 gcgaggtcca tagaattatt ttttactaag tttaaaagtt cggttatttc attagtacaa
    52321 atatccatag atatattagg gtatgtatta gaaaattctt ttaaatattg ttgaagtttg
    52381 tttttttcat atcctattaa ggttccaatg ctcaaagttc caaaaaaacc atgtgcaaat
    52441 tgttgtgttt ttgcaatagc atcattgata ttttttatta tagtacgtgc ctcagataaa
    52501 aaaacttttc cagctggtgt tagttcaaca tgacgtttac ttcttgtaaa tagagttaca
    52561 tttatttgtt cttctaaagc ttttatttgc tgtgtaacag cagtttgaga aatataaaat
    52621 ttttctgctg ttttggtaaa acttagattt tctgctacag atataaaata ttctaattgt
    52681 ttagtattca aataatcacc cctatttaaa cttgattgtt tgtaaacctt atttatctat
    52741 aattaaagct tattatagta taagaaaatt tacttttaaa gaatattaga aaatatttgt
    52801 ataaaaatat atgttttaag tatatcttta cattagattt tataaattta ttaataagaa
    52861 taacttatat attccaatga aaacgatatt ggatttaagt aagtttgtct cataaaatga
    52921 aagtatatca attgataata ctttaaataa gaagggagaa aaaatggaag ataaatttta
    52981 tgcaaaaggc aacggaaata acggatatat taaaaatctt gaagtttgtt cctttaataa
    53041 cttagatgga acttgtggaa tgtttcaaat ggctctgtac aaaagagatg aaaaatacta
    53101 tttatatgga tgctgttttg gaggaaataa aaaaaatgga gtaatgatta gcgatattac
    53161 agacccttat aatccacaat ttataaaaca ttttcaaatg ttagacccta aagagtatcc
    53221 tacaacaaca actcccaaaa ttcaaatagc agatgattta atgatagtag caatgagttg
    53281 tggaagtgga ccaggagcac ttgttgacca agctaaatta gcaaatatta agtgtgaagc
    53341 aggaattaga atatatagtt taaaagaaga ccctttaaat cctaagtttt taggatattg
    53401 ggattgtggc ttaaagcatg taatgggtgt tcatagattc atgtacaatg gtggaagata
    53461 tgtacattta tcaagtgatt gtgttggctt tgaaggtctg atttataggg tcatagatat
    53521 aataaatcct actaatccag tggaaatagg taaatggtgg agaccagacc aatatgcaaa
    53581 tggatatcca aatagaactt ttgatgcagg agcacctcat tgcccagaat ttatggataa
    53641 aggatggctt catggacctc catttgtaag agacggaaaa gcatattgtg gttatggagg
    53701 agctggttta gttgtattag atgttgaaga tttaacaaga ccaagatgct taggtgaatt
    53761 gccatttatg cctgcatttt ctagtagact tgcaggtgca agaactcata cagcattacc
    53821 attgccagga agagatttag tcgttgttca aaatgaggga gaaagattcc agttctttaa
    53881 accagataac attacagatg ttcaagctat gaataatata catatggttg atgttagtga
    53941 cccaacaaaa ccaacattaa ttgctcaatt tccatatcct gaagttccaa aagatttccc
    54001 ttatcctaac tttaatgttg cgggattagg aaaaccaggg ccatttggcc cacataatct
    54061 tcatgaacca atggataata agccatggtt agagcaaaga ggagatagag tatattgctg
    54121 ttatttccat gcagggctaa gggtttatga tgtatcagac ccatattata tcaaagagct
    54181 agcatatttt ataccaccaa atccaaataa aacaccagaa gaatcttatt tcccaggatt
    54241 cccaggacca cgcttggcag taacagaaga tcttatcgtt gatgatagag gctacatcat
    54301 catagatgct ttagatgatg gattctatat attaaaaatg aaagaagatt aaataaatac
    54361 taaagaggga gagttgaaaa tgaaaatact aggtatttct tttggaacaa aaaatggaaa
    54421 taatgacaca atgtgtaaag ttgcacttaa aggtgccaaa gaagcagggg ctgaagtaga
    54481 atttattcaa atgtcatcta ttaacattaa acattgtact ggatgttgtg cgtgtgttaa
    54541 aactttatta tctgggaaag gtagtatgtg tgtattaaaa gatgattttg aatggttgct
    54601 tgacaaaatg aaagatgcag atggtatagt agtttcagac cctatttttg aagaaggggc
    54661 atcaggatta ttccatacta taatggaccg ttttggaccc agagcagata gaggaaataa
    54721 cattatagga acaaaagttg cagaagctat aggaggaaaa attccagatt caagaatgct
    54781 taatgataaa gtaatatcat ttatgggaat aggaggttct gactggggta caagagttca
    54841 atgtgaacat gctatgctag ctctcatacc aatgtggaaa gttatagata atgcttggtt
    54901 cccatgggca aaggaattag taatggatga tacaaggctt gttcaagttc atcaaattgg
    54961 tttaaatata gttgaagcag caaaagattt tgaaaaagct tcatatcaag gagaagaagg
    55021 tgtatgtcca cattgccaca atagattatt ctaccttgag ccaggtacta aaaaggcaat
    55081 ttgtgctcta tgtggtatag ttggagagtt agatactata agtggaaaaa ctatatttag
    55141 ttttccagaa gagcaattag gtcatgctca tgatacttta ccaggtaagt ttatacatgg
    55201 tgatgatatt aagaaaatgg aaggtcatta tgcagaagtt cgtaaaacac aagaatttaa
    55261 agaaagaaaa acatcttata attttataga gccattaact aaagaaaagc aagctggagt
    55321 ttcattagga taataattaa aatactaaga ctctttttta aggtatgtta gatgttatag
    55381 gaggcttatt gtatgaatga aaagtcaagt tttaaacaaa ttgaaattat tttagcagtt
    55441 atttttttgg tacttacata ttacaaggtt agattaactc cagcatttat tgttatattt
    55501 ataccactat atttaagtta ttttgcagta tctatgattg taaatcaaat agcaattaag
    55561 ctattggaga taaggcaagc tagggaagct actaattaca atataggaaa tggatagaaa
    55621 gaaaaaagaa atattaacac tgtctatggg gattgcttta ctacctcctt tatgggcagt
    55681 attagcacct tatataggaa taaaaactgg agctgtagca ctaatctgtg caggattata
    55741 tgtaactaat ggtaataaac aaaaagatgg attaaaaata atgtttggtt tttggtgtgg
    55801 agatttatgg gcagtgttag ccattttaat tatgggatat atgaatttca atcaaaacct
    55861 agaattattt ttaacactta gtatattagg tttttttgca gttgtaattg catccttatt
    55921 tgaaaaaata attttcctac catcatggct ttgtggttgg gctataggtc taactattat
    55981 gacaggtgaa aatattatta atttacaaga tatttgtatt caaattgctg tagctatggc
    56041 agtaggagta tggtacgtgg gtgcaggtgt agatttgttt acaatatata ttttgagtaa
    56101 agataggaaa aagggaaatt gaaagcatgt gaatatactt tgattatagg agaatggcta
    56161 tgggttttga atttaaaata atgagaagtt taatatatgt aggacttgcc aaggaagaat
    56221 atagacctaa gctaatggac tggttatatc gtcaccatat tccagatagt attagcactt
    56281 ttggaccata ttgtactaaa tatgcctttt atcaagcata tcctacacca aatgaaggtg
    56341 agcgttttgg tgcacgtaag atgcaactaa cagaacatta ttggcttgta gatgaacata
    56401 tgcctgagat ggcaaataga attatgacag aatatatgcc tatggatgtt ctacgttggc
    56461 aagggtgtat accagatgta gaaaataaaa gggttcatga aaatgcagaa agtggagatg
    56521 caggacgtgc agtaggtgga gataatggat gtccaccatt tatatttgcc tttgttccaa
    56581 taaactggga agaagacttt agaggaaaag gacgtactgt acaagatgga ccaaactatc
    56641 gttggcaatt tatgattaag tatccagatg gtatctctaa agaagaagga gaaaaatggt
    56701 tctatgatga ggtagtgcca tactttacaa actgttgcta tgttaatcgt tttgtcagta
    56761 gtaaaataat gattaattat ggagcaactg cttttgaccg tgtatcagaa ctatggtttg
    56821 aaggggaaga agaatggtat aaagctgtgg ttgaagagac aaagtcgttt attaaaaaac
    56881 cagaatgggc acaagaagag gagttcccat atttaaaacc acaattcaat atcgcatcag
    56941 tattcttagg tgatatagca actatggatg catactcaca gtatcgtgga tatataccaa
    57001 tgagataaaa attatagttt gtgataagta gaggaggcat ttactcttac tggtagaaga
    57061 agacttattg tatccttaaa agtaggtgaa tatatagttt tatatttaaa tttatataaa
    57121 actaagacta ataaatatga aacaatagaa ttagaataat ttgataatat ttgttaaaag
    57181 agcatttaga atgtgtttaa agattaagat attcctttta tggtagcaga tagttgaaat
    57241 aataaaactg ttatcatgaa aggaatattt ttatgtatag gaaattttta aaaataaaag
    57301 ttaaatatgt ttaagaacat cttaaagtga aagaatcatt gttagaaata tgtagacaat
    57361 atagtcttta taacgtatca tattaacaag tttacacttg gattatgaaa taaaaagagt
    57421 aattccaaag gttgtaaata ctatgctgac tttgatactt caaagtttag gtgattttgt
    57481 aattgttaat agaaatacgt ttaatgagca taaagaattt atctaataat aggagaatat
    57541 aatgtataca attggacaag tatctaaatt tttaggaata tctagagata cactaaaatt
    57601 ttatgaggac aaaggattag taaatcctaa gaaaaatgat gaaaatggat atagaatata
    57661 taatcaggtt gatatctatg atattgcaac aataaacttt tatagagaaa ttgatataga
    57721 aattaaaaag attcaagaaa taagaaaaag taagagtata aacaatttag aactcttact
    57781 agaagagaag gaacaaatca tactaaaaga gatagaatat aaaaagcttc tcttaaaaaa
    57841 ggttcagtta gtcaaagaag attgtgacaa aataaaaaca tattttggaa aatacacaat
    57901 aaaagaaatg aagcctttgg aagtaaaagg taaaataaaa catgttacta actatgagga
    57961 atatgaagcc ttaaaagaga atacaaacaa cttaaaaaaa gcagttacat taactgattt
    58021 aagaagagta ataaatttta atgaagaagg tataatagaa gaaaaattta taattgtaag
    58081 ggaagtagat gactacaaca aaatcataga aggtgaagtt ctatctcatc ccaattgtat
    58141 ttatactatt attgaagatg gcagatggtc aactggagga gaaaatattg ataataaagt
    58201 ggaagacaga ttaagagaaa tagcaataga aaatgaatgt gaattacttg gaatagtcta
    58261 tgttaatata ttgcttacta cttatgaaga agggattgag cgtatatttt tagagattta
    58321 tgcacctata aaataatata aaagcattga cctgggactt actcataggt ttaaactcat
    58381 gatataagca gttataactt atatagcaat taaatattaa tttgaaatga ggattactta
    58441 tgagaacaga gaaatcatta aaaaggccta tagccagttt tattattcta acaaatataa
    58501 tttttttacc acttttttta ttggtagtag tgactataat gttaaaactt ccaacagtga
    58561 tatctgacat tgcgttgtgt atatcagcgt ggtcatccac ttttgcattt atgatattat
    58621 ttaaaaaaat ttatccaggt aagaaatttt tagtacacgt aaaagataga tttagaaata
    58681 aacttaaatt ttcaacagta tctataataa ttagtataca agtcctaata gctgtagtag
    58741 ttatatttat attagcatct aaaaatcatg gaataatgcc taactttaca gtttcatcat
    58801 ggggagcatt tatatattta tttttaaaaa atttatttgc aggatcatta ggtgaggaat
    58861 taggttggag aggatttgtt caaaatcatc ttcagaaaag acattctcct ttaaaagcat
    58921 ccataatagt aggtttttgg tgggggatgt ggcatttacc aatatggttt acaacaggat
    58981 tcaaaggact tgatttaata aaatatatta tattttttat gattgcaatt atatcaatca
    59041 gtattataat gacaactttt tataatctaa ataaaacttt gataatacct attgtaatac
    59101 atcaactatt taatttttta atagggataa ttaatggaga tttaatagag cttataaatt
    59161 actatgcaat gttatattta atatttgcaa ttgcattaat agttataaat ccaggaaatg
    59221 tattatataa aaatattaga gagtaatata tatctttatg gtaagatata aagatattat
    59281 tagaagaaca tccaaggagg aataaacatg gttttactga ttgttgatgc acaaaagtta
    59341 ataacaaacg agaggttata taaatttaat gaatttgtag taaatgtaga aaatttaatt
    59401 gatacagcta gaaaaaataa tatagaagta atatatgtac gtcatgatga tggagttgaa
    59461 aatgaattga ctaaaggtaa aaatggcttt gaaatatacg aaaaatttaa accatgtaat
    59521 ggagaaaaaa tatttgataa aaaagtaaat agtgctttta aagaaactgg tctattagaa
    59581 tacctaacaa ataatggtga aaaagatatt attattgctg gtctccaaac agattattgt
    59641 attgatgcca ctataaaatg tggatttgaa catggattta atataatagt tccagcttat
    59701 tcaaatacaa ctgttgacaa tttttttatg tcagcagaac aatcatataa atactacaat
    59761 gaatttatat ggaatgaaag atatgcgaag tgtatttcat tagaggaaac acttagaaaa
    59821 atgaaataaa aatagagtat gaattgtata tgtacataag ttgatttcat aaaaaaggat
    59881 gttaaattcc aagttaaaaa ttagattaaa tatattatat tatagataag gcacatcaat
    59941 tttattttaa attaatgtgc tttttaattg tagtaattta tttataatca atatgatttt
    60001 tattatttaa atcaattttt cttgagtttc tttgtctaaa gaaacttttt ttatttataa
    60061 aatctacagt tatattatag tatctatttt agagaagttt aatttttgta tcaatctaaa
    60121 acaaaagtta tacttaaatg aaattgaaaa cgataaaatt ctcaaataaa ataagtattg
    60181 tggaggtgat gaaagaatcc aatgataata tgaagactaa tcttaataag aaagtagggg
    60241 ttaatggtag tggacaaaga aattgtatca attctccaga ttttattaaa tagcacattg
    60301 attacaacta atggattaca agaggagtct aattcatcaa agagacaaat tacttataga
    60361 ataaataaaa tcaatgacat gcttaaagta aaaaatgtac cacttatatg tttaagagct
    60421 gataaagata ttatcgttag aaaagaaaca aaaaaagcaa ttaaagaaat tttagaaaaa
    60481 aactattcta agaatactta ttattttagt aagacagaaa gacttttata tatgtattta
    60541 atgcttttta ttaacatgga tgatatttca attaatcatt ttataaattc tataaaagta
    60601 agtaggtcta ctgtaaatct tgatttcaag gatttgatac cagagcttga aaagaagaac
    60661 attaaagtta agaacaatcg tataaatgga tattacatag ttggcaatga gatggaaatt
    60721 aggagagtgc ttattaaaaa tattatagaa actttgtcta atgagaaaag ttcaagagta
    60781 tttgatattt ttattaaaga atataattta gatagttttg aagaggctaa aaaaattata
    60841 ttaaagctaa taaaaaaata tgaaattact tttgttgaag atagattagt agaatttata
    60901 tatatattta tctttctaaa ggcaaggatg tattcaacta gaatagttac tcaggaaaat
    60961 gtagatattc ctaatatttc agttatgagt tcaatgaagg agtataaatt tgctaaggaa
    61021 ttattagaag tatatgggag tgaagataag ataaagcctc acaatataat gtatattagt
    61081 gcttggattt taggtatatc agtaggaaat tatgaagaag atacagaaga taaggatgta
    61141 atttcaaaaa ttgtaaataa aatgatgaca aagtttggat atttaagcgg agtttactat
    61201 ataagccaag aaaaaatatt taaacaatta tattctcatt ttagacctgc ctactataga
    61261 cttttattta aattacctat ttataatccg atttgtgaaa aagtaaaaga agaatataaa
    61321 ttagtttata gaattgtaag taatgcaatg aaagagtttt gcggattatt tggagaagaa
    61381 attcaggaag aagagttagc atatcttaca atgcactttg caacactttt ttcaaataaa
    61441 aaagaatttg atggttttag aaaaagaata gcattaatag tttgttcaaa tggcgttggt
    61501 agttcagcta ttttacatgc agaacttaca aacttatttc ctgacttaca ctttatacca
    61561 atgtcggaat cagttggatt agaaaatata agtgaacctg tagatattgt atttactact
    61621 aattataacg cagagggttt aaaaacagat gtaccagtaa ttaaagtaag ccctgttatg
    61681 accacaaaag aaaaatataa gattacacgt gaagtgtata ttcaattagg tgatattttt
    61741 ttaagacaac ctaaaataga tgaggttatg gatataataa aaaaatatgc aaatgtaacc
    61801 tctgaaagta ttctttcaag tgagctatta gcttatttta cacagataga gaattttaca
    61861 tctaaggaag gagaggggcc tatgctaagt gagattacta atgaaacact agtaaaaata
    61921 aaggtaaaag ctaagaattg ggaggaagct attagacagt cagcttctgt attagttgaa
    61981 aataacaaag taactgaagg gtatgtagat gctatgatta actcagcaaa agcatctgga
    62041 ccatacatag taattacaaa acatgttgca cttccacatg ctagaccaga agctgggtca
    62101 aaagagattg caataggcat tgctacttta gatagtccta ttgagtttgg gaataacgaa
    62161 aacgaccctg taaaatatgt attttgtcta agtgcaatag acaataatag ccatttacgt
    62221 gctatgtctg agctagttga actattggaa gaagatgagt tttttaaggt tttagatagt
    62281 gctaaagaag ctaaagaaat tattgatttt ataaaaacac acgaattata aaggaggatt
    62341 taattatgta taaaatttta atcgcatgta gagcaggagt tggctcaagt ttaatgttaa
    62401 aaataaagac tcaacaaatt attaaggaaa ataactttcc aattgaagta gaacatggtt
    62461 cattggattc actaaatgga ttcaatggag atgctgtaga aacattaatt gatgttgctg
    62521 aagaattaaa aaataaaaat ttgaagtttg atataattgg gattcacaat attgttgacc
    62581 gtaatgaaat aaagacagca ctagaaaaat tccttgcttc aaaagaggga taaatttaag
    62641 atgataagga ggaatattta tggagtttat tattagtttg ctgagtacac ctgctgtttt
    62701 gcttggcgta gttgcatgta taggattgat tcttcaaaag aaatcagctg ttgaagtatt
    62761 tacaggctct tcaaaaacac ttatcggttt tttgatattt ggtattggtg cctctgctat
    62821 gacaggagca cttcaaaatt tcaataaact gttccaacat ggctttggaa ttactggtgt
    62881 tatagcttca ccagaagcag ctacagcact tgcacaaggt acatatggtt ttgcagtttc
    62941 atgtacatta attcttggat tcatattaaa tttagtattt gcacgtatta caaagtttaa
    63001 gaatattttc tttacaacag gtcatagttt gttcttttct tgtgtattag ttttaattat
    63061 gaaagcacat ggatttgata atacaatcac tattttaata ggtggaacta tcttagggtt
    63121 tatgtctgct gcactacctc aattctgtca accatttatg cgtgaattaa caggtggcga
    63181 cgaacaagct attggtcact ttaacatgat tggttatggt ttatcaggtt atattgggcg
    63241 tttattttca aaacataaag atgatacaac agagactata gaatttccta agtggttatc
    63301 aatattcaga gacttcttaa tgggtctatc catagttatg ttaatcttat tttatattgc
    63361 aacattaaaa gctggtcaag catttactca agaaattgct ggttcaacac attggttggt
    63421 ttttccgctt atagaggcat ttacatttgt ggcaggtatg tcaatattga tgtcaggagt
    63481 tcgtatgttc ttagctgaaa taactgcagc atttgtaatc atttcagaaa agtatattcc
    63541 aggttcacgt cctgcgctag atgttccaac cgtatttcca tatgcaccaa atgcagttat
    63601 tattgggttt atatcagcat atgctgctgg tctattagca attgctatta tggcatcatt
    63661 tcctagtata tttcctgtag taattattcc agctgctcac atatgtttct tctcaggagg
    63721 tacagctgca atatttggca atacttcagg aggatggaga ggggctattg ctgggtcatt
    63781 tgttgtgggg ttattacttg catttttacc agttatacta tatccagttt atgggactct
    63841 tggtatagaa ggagcaactt tccctaatat tgattacaac gtagttggtt cgatattaca
    63901 taatatttta caatttataa aacctattta gactaaatta tataaaagta tagtcatagt
    63961 tactatgatt tcaaggagga tacatcttat ggaaaataca ttatctgaat ttttagataa
    64021 tatatctaat gaattgtcag aatttattgc ctcaatagat gagacatcta taaataaagc
    64081 atgcgaatta attcttgaag ctgaaaaaaa tcacggacgt gtacatgtga ctggtattgg
    64141 caaaccagga catgtatcgg gatatatttc atcattgctt tcatctactg gaacgtcagc
    64201 atatatattg catggtacag aagcagtaca tgggtcaagt ggtcaagtag ttgaaggaga
    64261 tgttgttatt gcaatatcaa atagtggtga aactcaagag ttaaaagcaa ctcttaagac
    64321 tctaaaagta aatggagcaa agataattgg tgtaagtggt aatgagagtt catttttaaa
    64381 aaatatttct gatatattcc tgtttgcagg tgttaagcag gaaggagatt gtctcaataa
    64441 agctcctcgt gcttcaattt tagctgaaac aataatatta caatcattaa gtgttgtatt
    64501 acaatatgct aaaggtctta atacacagga gtatttgaag tggcatccag caggaagtct
    64561 tggaaagtct attagagagg gaatataatt ttatgaaata taaagggatt atatgtgcaa
    64621 tgataacacc atttgatgaa aatcaaaata ttaattcaca agcaacatgt caattaattg
    64681 attatttgat tgaaaaggga atttatggat tatttatttt gggcactaat ggtgaatgtc
    64741 atgtattaac agatgatgaa aaagttgaat ttgcaaagat tgttattaat catacaaata
    64801 atcgtgtacc tgtttttgtt ggaacagggg gtaattcaac tcgtgaagtt gtagatttat
    64861 ctaagagaat ggaagcgatt ggagcaagtg cattatctat tattacacca tactttgtta
    64921 caccaacaca acaggaatta atcttacatt acaaaactat tgctaattca gttaatttac
    64981 ctattataat gtataatatg ccaggtaaga ctggaattaa tattgaacca gagtcagtac
    65041 gtgaattatc aaagataaag aatattgttg gaattaagga tagtagtggt aaacttgaca
    65101 acatgaaagc ttatattgaa gcaacgaaga atgagaattt tagtgtgttt tcaggctcag
    65161 attcacttat tctggatagt ctaaaagctg gtagccaagg agctgttgca gcaacaacaa
    65221 acttcctcac agagattggt atctctatct atgaaaactt tatgaaaggg gatttggaag
    65281 aagctcaaaa atctcaaagt agtatagaag aacttcgacg tatactgaaa cttggaacaa
    65341 ttccatctgt tatgaagaaa actattgtct tgaatggaat taatgttgga actgcacgac
    65401 ttcctgtaac tgaaccaact ggggaagtac ttgaagaaat taaacgagta gttgaaaatt
    65461 ataggacaat acttaataaa agtagtgaga atagataata taaataggga aattacactg
    65521 gtatataatt aatatatata ttataaaaca tgactaaaat taatgaataa gtaaagtggt
    65581 ttgatgagta tagaggtgat aatatgaaaa agatgaatat aaccaaaaga atgtcagaat
    65641 gtggggctct tgcaattgta agagaagaaa atcttaaccg tgcatgtgaa attgctgaag
    65701 gatgtataaa aggtggtata acagttatag aaatgagtta taccctaaat aatgctggag
    65761 aaataattca agggcttaat aaaaagtatg gtgaaatact ttgtgtggga gcaggaacag
    65821 ttttggatag tgaaacagct cgtcatgcta tattacatgg agcccaattt attattgcac
    65881 caaattataa tgaaggtgta gcgaagattt gcaatagata tcagattcct tatgctccag
    65941 gatgtacatc attgactgag gcagtagacg cactaagttt aggagcaact tttataaaag
    66001 ctttcccaat atcagatttc tatggaccta aactagtaaa ggtatttaag acaccaatac
    66061 ctgatatgcc agttttagca agtggagggc ttaatattga aaacctacat atatggttag
    66121 aaaatggagt tgatgtatgt ggatttggtg gtttattaac taaaggttct gttgaagata
    66181 ttgctaaaaa tgctaacaag attcgtgaaa tcattaaaaa tgtaagaaaa atttaacata
    66241 gggctgtatc atagtatctt ataattacta tgatacagcc tttttaatat tattcaaatt
    66301 taaaacagtt tacatttcta ttatattaca tataatttca gtaattttat cttaattttt
    66361 ctatctggaa aaaaatgatt taaaacttaa gttatgatat aatttcatta aaagaaggcg
    66421 atttttagta aatatgagaa aataatgtgt ttttagggga aaagttaaat acagatttaa
    66481 gtgaacaaga atcgataatt aagatattaa atggtgaata gggcatcaaa aaaattacta
    66541 aattaaaagt tttagaggga gaatcttagc aagtgtgtgt gaaaattcaa tttcaaacaa
    66601 aaataattgt aaatgaaatc aatttataca cgttgaagat tcacttttat cattatatta
    66661 aaaagcagag tgaattaaaa gtgagtctaa agataggagg ataattaaaa caatgaaaga
    66721 agacattagg ttacatcgaa tgtgtaaaaa agaacaagca acaaagaatg aagttatatc
    66781 agaggatatt ctaaaatcta tgagtattgg ggtcagtaaa cattttttag atgattcatt
    66841 tacaataata tggggaaata catcattcta tcaattatta ggatatacag aagaagaatt
    66901 tttagttaaa ttttctagtt taaaagatta ttatgaacag aatctttatc attttgaatc
    66961 tatgaaaaat cattttttag aggcttatca tcatggcgaa aagagtatag aatatcaaat
    67021 ttgcatttca gcaaataatg gagaccctgt ttgggtaata atgactggta tattaattga
    67081 atcacaagat aatgagaaat caatggtcta tatattgtat tcaaaaatta atgagttaaa
    67141 aataaagcaa gaagaactat ctgatatgga aaaagagagt acagagaatt ttaaatggat
    67201 gatgtcagag tatgctggga atgtttatat tagcgatatg gatacatatg aattactata
    67261 cttaaataag cactcatgca atactttaca ggcatcagca aatgaactta ttggaagaaa
    67321 atgttatgag gtaattcaag gtagaacttc gccatgtccg ttttgtacaa attcgtattt
    67381 aaaaaaagat gacatatatg aatgggaatt ttataataaa aaactgaaac gaacatttat
    67441 aattaaagac cgtatgttga attggcaagg gcgaagagtt cgtattgaat tatcttatga
    67501 tatgtatagt gctgaatata aattagcaaa aaaagatcag gaaagagaat caattttgaa
    67561 aactattcca gcaggaatgg ttagattaga tgcaagagat tatcttacta tactttggtg
    67621 taatgatata tttttgaata tgattgaata tacaaaggaa cagtttgcag aggaacttaa
    67681 taatcaatgt ggatatttat tttctgaaga ttataaacgt agtaagaaac tggcacaaga
    67741 tttgaaggag tctggggata atgttgtatt tgaagcaaaa atatatacac gttcaaggga
    67801 agaacgtata tggactgtta cattgtgtta tatcagtggg gaagacagtt gggatggaat
    67861 accatctttt tatagtatag gtattgacat aacaaaggaa agaaaacaga ttgagaaatt
    67921 acagcatata tcagagaaag atgcattaac aggaatttat aatcgtgctg agactgaaag
    67981 gcagattaag aaatattttg aaaaaaattt aaatgttatg ggtgcactgt ttatgattga
    68041 tacagataat tttaaacaga ttaatgatac agaagggcat atgattggag atatagtact
    68101 tactgagatg gcatcaggta tgaaaaaaat aatgcgtgac agtgatgttg ttggaagaat
    68161 aggtggagat gagtttacaa tttttatgaa gaatatttcc tcagttaaag atgcagaaaa
    68221 gaaagcagaa gaactattgt atatgttccg tcatctattt cagaaagaaa aaagttttct
    68281 aaaagtaact tgtagcatag gaatagctat atatccaaag gatggtacta cattcaagga
    68341 aatatatgca agagctgata aagccctgta tcaagcaaaa aatatgggaa aaaataatta
    68401 tgtaatctac aaccaagatt attttaaaga attagaagaa tttgattgtt cttctcttgg
    68461 tacagttatt gattcagaaa aacaatacgc tgaatatcct gataatctta cacgttatat
    68521 attccgtatg ctttatcaga ctgatgatat agaccaggcg attaatatga tacttgaggt
    68581 tgttgggaaa cagtttgatg taagtcgtgt ttatattttt gaaaatacag aagatggtag
    68641 atatacttct aatacttatg aatggtgtaa tgaaggaatc tcttctcaaa tggcttattt
    68701 gcaaaacagc aattatcaag actatgagga ttatgaaaag ctttttgagg atgaattagt
    68761 tttttattgt cgagatatcc atacattaag tcctaaacta gaagaattgt tttctagtca
    68821 aggtatccac tctacacttc aatgtgcata taaagaagat agagttttca gtggatttgt
    68881 tggatttgat gaatgcacag gactacgtct atggtcacaa gaggaaataa gtacattatc
    68941 attgatttct caaattatat caatgttttt acaacgtaaa aaaaataata aacttaatca
    69001 acagattcaa caataccaga atattttaga taatattgac cagtgtattt gtgtgattaa
    69061 taaagaaaat aattttttat tatatgtaaa taaaaaattt aaaaatacat attctaaatt
    69121 ccaaattgga caaccttatt atcttgattc tatagatatt cataagatat ccattatatg
    69181 gaataaaaaa gaagcatacc tatgtactag cacgatttaa tggtaaagta aagtttatat
    69241 tggacttaaa ttaaatttcg ttttaaataa gatattattt tagttgctta ctgaaaagac
    69301 tagaatctat attaatgaat tatactaatt ttataccact attaaaatat tgtttgctaa
    69361 aaataaaaaa tagccttaaa acagttgatt ataaaaaatt aactgcttta aggctatttt
    69421 tatctttgtg acgaaataga cacttgccat ttagaatgtg acaatatagt agttgtttag
    69481 aaaaggcaca aagcattata ctttaactaa agtaaaatgt attttgcttt tagaataaaa
    69541 acaattagga gggagctata attgaaaaag ataatatatg tattatttac aattgaacta
    69601 ttaatagtat ctttattagg tctaaatata gttaaagata atgaaattaa taatatttta
    69661 tataacgata caacaagtat atctgtaatg tttaaagatt ataaaaagtt aaataaaaat
    69721 tatagtaagt ggattaaaga tattgcagat gaaaataatg tggctatatc aaagtatgtt
    69781 tttaataata atgaaaaatt atctatatat acgactgata caagtttgaa taacaatata
    69841 aagttaaaat caggaaaatt tcctaatact aattcaaatg aatttattag taatataaac
    69901 actaataata gtaatcaagt aggtaaattc tcaactatga gtaaagacat atcaatttta
    69961 attagggatt ttggtaaaat aagtgaagtt ggagaatcag gaatcttata tatttcaact
    70021 caaaatgaag atacaataaa taaaatatta agagaactaa atgtagacag aagtattttt
    70081 gtagcaagat tacatgatag atatgttaac tcaaatttat acttaaatcc ttcaataatt
    70141 agagatttaa tactaattat cctttgtttc ttggctacaa taatacatta ttcaattagt
    70201 gtgagtagag aggcttctat tttaagatta aatggatact ctaagctaga tataattgct
    70261 agagttataa aaaatatact tagaattatg attttatcat ctgtcacagc attattaata
    70321 tatataatct atattttaaa attaaatata ggtgttagag catgcttata ctttgctatg
    70381 ttttcgatta tatgtatttt gataaatatt ttgatatcta tattaatagt tggttttaat
    70441 agtagaagtt ctaaatatgt gctatctcta aatggaaaaa aatcttatgg ttttgtaaat
    70501 ataatgcatt tcacattgaa aattgtattt gtatcatttc ttatattatc cataaataac
    70561 tgtattttaa attataatat gctacaaact cagttaggaa atctatctga gtgggataaa
    70621 gcaaaaaatg tttataatct tactcttaaa gatacaggcg aacaatcttt aggaaaggaa
    70681 gaagtaatta aaaatgccaa gattaaaggt ttctataaaa atttggtaga agaaaaagat
    70741 gctttcttaa tagattcaac taattttgaa aaacttgaag atggaagtta catgtatgaa
    70801 ataaattcaa agggtaaaaa cccagaagta agtcaatatg gtaaaaatat aaaaataaat
    70861 aaaaactatc ttaaagttaa ccctatttac tcaaatggca aagaaatatt taatctaatc
    70921 gattatgacg ataagactat aaatttatta gttcctaaaa agttacaagt acatgaaaat
    70981 gagattaaaa aagagtttag agaattattt tattttgaaa aaatagaagt agaaaatatg
    71041 tacaataaag agttaaatag agagttaaat aaaacaaaga agtccgattt aaatgttaat
    71101 ataatatatg tagataataa tcaaagctat tttacttata actcccttgt tatggatgac
    71161 aataggaatc tcattgatga cccaatagca atagttgaca tagataatat tgatgattct
    71221 ttttatcttt cttatatatc tagatgtgta tattttaatt ctaagaaatt agatgccctt
    71281 gcagatataa gtagcattat tgaagctcaa ggtgtagaag cgcatatcca aagtttacat
    71341 gctgtttata atgagtatgg tttagaaata aataaactag aaaaatattt gaatagtgaa
    71401 atttttacta taataataat agcaatatca aacttaatga ttacctacaa tatagtagct
    71461 agctactatg aaagaaataa atataagtta tatattaaaa aaatatttgg ttactcaact
    71521 acagctagaa gtatgttgtt aatagtctca ttaatattaa caaacattat tccaataggc
    71581 attatatcaa caagagtaga tttaagtaat aatattatct tatttggact tctaatatta
    71641 gttatagaag taattgtatc tattgtatta gacaagatta tctcaaatag ttcatttaat
    71701 aaaattataa aaggagaaca ttaaaaagat gatttcatta ataaatatat ctaaaaaatt
    71761 taaagataaa tctataatag acaaatttaa cctagaaatt tctaagggcg agtttgtagc
    71821 gataacagga aatagtggtt caggtaagac aactttactt aatataatgg gattattaga
    71881 aaaacctaat tctggtgata ttataataaa taatataaaa aatccaaata gtaaacagat
    71941 aagaatttta caaagggatt tttatggcta tctatttcaa aattatgctt taattgagaa
    72001 tgaaaatgtt gaaaataact tgaaaattgc tttaaagtat caaaaaaata tcaatcataa
    72061 tgaaaaaata aattacgcac ttgagtctgt aggtcttaga aaatacaata aaaaaaagat
    72121 atacgaattg agtggaggag aacaacaaag ggtagcatta gcaagagtaa tattaaaaga
    72181 atgtgaagta atatttgcag atgaacctac aggtaattta gataaaaaaa atagagacat
    72241 agtatttgat atacttaaaa gtctaaataa gaatggtaaa actatcgtat ttgtaactca
    72301 tgatttagat ttatcggagc aggcagatag agttataaaa ttgtaataat ataaactata
    72361 ataaatataa gaaagagatt acagctaaat taagtttaat aataatacga atagaattta
    72421 aacattaagt aaattagtag tttgtaaata taagtataat atgatatcat aaaggctcaa
    72481 aagaaatttt atataataag aaatctaaaa ataacaaatg gaggacatat aaagatggaa
    72541 aatttaccaa attgcccgaa atgtaattca gaatatactt atgaagatgg aactctttta
    72601 gtttgtccag aatgctctca tgagtgggca ttaggattgg gagctgaaga agattcaaat
    72661 attatcaaag atgtaaatgg aaatgtatta aatgatggag attctgttac agtaattaga
    72721 gaccttaaag taaaaggaag ttcatcatct ataaagattg gaacaaaagt gaaaaatatc
    72781 cgtttaatcc atgactcatc agatggacat gatatagaat gtaagataga tggttttgga
    72841 gctatgaagc taaagtcttc tgttgttaaa aaagcgtaat attataaata agctgtagtg
    72901 tttattgttt ttcagtacag tataaaacca taagaatcta ttaagtaata aaagtttgtt
    72961 tatttatctt tatattttct tatggtttta ttaaatataa attttattga acaccaaaat
    73021 attctctcct ctacaaataa gccaatctta tttaaactaa tatactttgt actatttata
    73081 ctgaaattgt aaatttaaat gctatgttat ttaggtttgt atagttaatt tatatatttc
    73141 tgattttata aattgtattt attattatat cttgattcta aaatcattaa aaatttcaac
    73201 ttctataaag atttacaatt tatcaaatac attatgagta tataaacaat ttattattac
    73261 agtgctacat ttaaaataag tcttagtata aaggtattgt tacaagtata aagaaactta
    73321 tgtatgctat tggaataaat aataccttaa attataatta agaataagaa ctgttaaata
    73381 agggacttga ttatcattta gttatagata atataatatt aaaaataaag tttaagagga
    73441 gaaaaaatat gatagatata cataaaaata tatatgataa taagttattt gaggagctaa
    73501 agattgactg taagaagtgc tttggacttt gctgtgtagc tctttatttt tctgcttcag
    73561 atggatttcc tatcaataag gaatcaggaa aaccctgtat taatttacaa ccagatttta
    73621 aatgttctgt acataatagt cttattaagc gaggttttaa agggtgtact gcatatgatt
    73681 gttttggttc aggtcaaaaa gtagcacaag taacatataa ggggatagac tggatgcaat
    73741 caccagaact aacaaatcaa atgtctgaag tgtttttaat tatgagacaa ttacatgaaa
    73801 tgttatggta tcttaaagaa gcatctgtat taaatatatc agatacaatc aaatcaaaaa
    73861 ttgatttaat aattgaagaa actgaaaaga taactaatat gggtccagaa cagataatta
    73921 atttggatat catatcacat agaacaaagg ttaatttatt attatcacaa gcgagtgagt
    73981 ctatgatggg aaaggttaaa tcatttatca agacttctac tcttaaaaac atgaaaaaat
    74041 taagtaaaaa tatagattta ataggagcag accttagaaa aataaatcta ataggagcag
    74101 atttaagagg tagatttcta atagcagcaa atttaagaaa tacagatttg agtggggcaa
    74161 atttaatagg agcagactta agagattgtg atattagagg tgctaatctt gaaaatagta
    74221 tatttttaac acaattacaa gtaaatactg caaaaggaga ttctagtacg aagttaccag
    74281 cttcactaat acgccctaaa tactgggaaa aataattaaa ttaatacata attttacaaa
    74341 atatgaactt ttttgacttt aattcatatt atacattaga gattctttac aagaatttct
    74401 taatataata tagggagaga tataaaaatg agttatcaaa attataaatg tgaaagagat
    74461 tatgtgtatg atgattataa atacaataag tgcaacaagt gctataatga ttatgaggaa
    74521 atgacacatg ttcatgaata ttcagaaagt gttaagttag cagaagaatg tgaggataga
    74581 cataaccacc gtgcagcagg ggttactggt gaagttatac caataaatgg aggtacaaat
    74641 catgttcata aaataaatga taatgtagat ttccttgacc atttccataa gatatgtgtt
    74701 acaacagggc cagcaattag aatacctgga actgataaac acattcactt aatatgtgga
    74761 gaaactactg ttaatgatgg tcattgtcat aaattccttt ttactactca aatagaagca
    74821 cctttagtat aagctgatgt gaattattgt agtgaatttg ctagattata gtataatata
    74881 taatagataa tatttaaact taatgaggac tatgatattt aaccttcgta gtcctttcta
    74941 agttttgtat aaaaaagtgt tatgcaatta aaagtgatat agggaggaaa tttttatgaa
    75001 atctggaata attcttgaaa tgaaacaaat aaattcaaca gaatatgacg aaatgttgca
    75061 agtctgggaa tcatcagtaa gagcaacgca tgatttttta acagaaaaag atattgaatc
    75121 tttgatacca ttagttgaga ttggacttaa agaagtagaa aatatagttt gtataaaaga
    75181 taatgatata ataaagggct ttataggaat agataaagat aaaattgaga tgctctttat
    75241 agaagataaa tatagaggaa atggtattgg taaaaaatta ataaaatatg ctatagataa
    75301 atacaatgta aaatatgttg atgtaaatga gcagaataaa aaagctgtag gattttatat
    75361 tcacttagga tttaaagtgt ttgataggtc tgaaatagat ggacaaggga acccatttcc
    75421 aatactacat atgaaattaa tttaaagtta tacttcccac ttattcgcaa aaatatttac
    75481 ttaaattttt atacaaataa aagtcgtgac agtcttagga cacgactttt ttactacact
    75541 attaaattga aatttatctg ttctatatta tgattattag atgtagtttt tttattttaa
    75601 ctatgtactt aaataccaag tatacccata cctatcaagt acttcagcag cgccatcatt
    75661 ttgatgtggt ggtgggtgtg gtaaaaattc ttgtacaagt gtaccacctt cggcaagttt
    75721 tacagatatt tcttctatcg cttctcgaga tggtagaaca acacaaatcg ccatgccaga
    75781 aattacaaca ggcttgtctg tatccgcacc cattatacaa ccttcgtatt ttgtgaaagt
    75841 aacacttgcg tgcataatag gattgtttgg attgctgtct aatcgcgcaa atatagtttt
    75901 tcctcctccg aaagcctcag cataaaaatt aaatgctttt tcacaagtac cattaaatat
    75961 taaatatgga gttagcatga ccaatatatc ctccttgata tagaataatt tatagttgca
    76021 cctgataatc taaattatga tttagtaaat tgtatcatag ggaaaccttg aagtatataa
    76081 ttttttatta ttatattata caaatttatt aatttaaaaa aatgttatat gtacggaagg
    76141 tttacaggat aaaatataat aaaaagatta aggtgacatt ataaaaaatg tatattgaca
    76201 ttgacgctac gtaattgtat atactttgtc ttaaaggaga tggtaaatat gaatattata
    76261 gaaattcgct cagataaaat atacaagaag ataatggatg caccaataaa caaaaaagaa
    76321 gatatataca gatatgaatt aatgaagcct tttgaattta agtggaagtg tatgaatgtt
    76381 ccaatagttg ctagacagaa aggtggatat gatgtaatta tagcaagtga aatgttaggg
    76441 gttttatcgc ctaaggatat tgatgaaaag caaaaaaaga atataaatgt gttatctgct
    76501 gataaaattt ggggcacttg caaagaaacc atagaaaact ctataaatgc ttttataaaa
    76561 gaagggtatg atttaaacat taaggactat aaatattcaa tattattggc gaatccaaat
    76621 agtccttata caatattaag tgatggatac tggggtgatg gtgggattcc tggatatata
    76681 tttctatcat tggttcctaa tgaatatact atcaatagat taccagtatt aatagcacat
    76741 gaatgtaatc acaatattag atttcagttt atagagtgga ataataatat aacattagaa
    76801 gaaatgatga taaatgaagg tcttgcagaa aattttgcaa catggatgtt tggagaggaa
    76861 atgttaggac cttgggtcag tagaacagat atcgaaacat taaatactta tataaagcca
    76921 ataataaaaa gtgctttaaa agaaactgga tttcaaaata taacatctta tctttatggt
    76981 gatgatatag ctaaaatgca aggatatttt ccagtagggt tgccttattg tgcaggatat
    77041 gcttgtggat attatatgat taagtattat ttagaaaaga caaataaatc aataatcgaa
    77101 gcgactttat tgccttatag tgagataatc gaagcagtaa aagagttttg ggaataaatt
    77161 ttataaattg cataatttga aataaattat gcaattattt tatttttaag aaaaaataaa
    77221 ttgacaataa tttataagaa atatacaata atatatataa agaaatattt caatatttaa
    77281 aagttttaag atttcatatc tagagtagct aacattacat gttagtttct tgttacttat
    77341 ctaattactt tatatttcgt agttatatat aaaattacta taaaaaatga aatatattaa
    77401 acaatcaact gaaaaatgaa atgttaataa aaaaaaattc aaattataaa tattaaatga
    77461 atataattaa aacaaatata taaatttaag tataacactc acattttaat gtattgtgaa
    77521 atttttgttg aactttatgt accataactt ataaaattta tgtcatgtta tatttgaata
    77581 agtaataaaa ttgtagatgt aaattaatct acaaaattta tatgaaatgg agttgatgct
    77641 atgacagaat taatgattat aagtgggtta gtactattga tttgtataac ttctagcaag
    77701 gtattgtata aatttggagt accaatatta ttgattttta tcttactagg gatgttattt
    77761 gggtctgatg ggatggtagg aatctatttt gataactatg aactgacaaa acaattatgt
    77821 tctattggac taatatttat aatgtttttt ggagggtttg gaacaaactg gaatatggct
    77881 aaacctgttg ctataccttc tatattaatg tcttcactgg gtgtaataat tacggctggg
    77941 gttacaggta tgttttgcca catggtaata ggaactagtt tacttgaagg tttattaatt
    78001 ggggcaattg cagcttcaac agatgctgca tctgtgtttg ctgtgttgcg ttctcaaaaa
    78061 ctaaacttaa agggttcact tgcatcatta ttagaagttg aaagtggaag taatgaccca
    78121 atagcgtata tgctgacact aattcttttg acattgatga ataattctgg ggtagaactt
    78181 attgcaccaa tggtaataaa acaaatagct tttgggttag ctataggatt tatactagca
    78241 aaattatctg tttatatttt aagacattca aacttcgaga tagaaggatt ttatactata
    78301 tttataacag ctattgcaat actttcttat gcatttagcg agtatcttgg aggaaatgga
    78361 tatttaagtg tttacatttc aggtattatt ataggaaata gtaggatacc tcttaaaaaa
    78421 agtttggttc atttctttga tggaatttca tggattatgc aaataatgtt attctttatg
    78481 ttaggattgt tatctttccc ttctaagtta ccaaatgtat ttggtatagc aatggccata
    78541 tctatattca tgatagttat tgcaagacca ttagcaacat ttatagttct agcaaaattt
    78601 aaattctcaa ataaagaaaa attattcata tcgtgggtag gtcttagagg tgcggcttca
    78661 gtagtttttg caatttatgc tgttactcaa ggagtagcca tagagaatga catattccat
    78721 ataatatttt ttatagcgtt gctttcagta ggaatacaag gttcattgat accaactgta
    78781 gccagaaagt tggatttagt tgatgataat acacctgtat taaaaacatt taatgattat
    78841 gatggagaaa ttagctctaa acttatcgaa gtaaatgttg atgagggtaa taaatgggct
    78901 aataagagta taatagattc taatatacca gacgatatat taatagttat gataaaaaga
    78961 aaggaacagg ttttaatacc taaaggttca accattataa aaacagggga tattttggta
    79021 ttgagtggag atagaattga agaattaata caattataat ttattttgta ttgactttaa
    79081 cctaagggga aagtttaaac ttaaattaat caaatctaat gcgcaggtga tgagatgttt
    79141 tctataggaa tgttttctaa aataaataaa ataactacaa agacattaag gtattatgaa
    79201 gatatagggt tgttaaaacc agaatatgta gatgaattta caaaatatag gtattataca
    79261 acagagcaat tacctaaatt acatcagata ataactttaa aacaaatggg tttatcatta
    79321 gctgagataa aaaaagctgt agaaaatcct gaagaagttg aaaatatttt aatgaataaa
    79381 gaaaaagaaa tgttggatgt aattaaaagt gaagaatata aaataattaa actacgtaat
    79441 taccttatga atttgaaagg agaagtcaat atgaatgatg taattattaa gtctttacca
    79501 aaagttaaag ttgcttctat gagaaaagtg ttaaaggggt atgatgaact ttttaatcta
    79561 tttccaaatg taatggctga agaaatgatg aaaacagggt gtacatgtgc agttcctgat
    79621 tattgtttta atatatatca tgatggtgaa tatagagaga ctgatatgga tgttgaaatg
    79681 tgtcaagcta taaatgattt aaaggaagat acggatatat taaaatttaa agaattagat
    79741 gcagttgaaa atgcagtttg tatattacac aatggtccat attcaacatt aggaaaaact
    79801 tacgcagatg catttaaatg gatagaggat aataaatata aagtggttgg aaatcctaga
    79861 gaatcttata tagatggtat atggaataaa gagagtgaag aagactggtt gacagaagtt
    79921 cagataccag tgaaacgcat gttataatat gtctacaaag tataaataag aataaaaaag
    79981 gtggtgtctc aaagtaaaca ttttagttta tgagacactc tttttcatat gaaatcaagt
    80041 atatttttat aattacgatt atgaaaaaag gcttatctat atttttagac agcctcttat
    80101 ttttttatta taatagtttt ctaaagatgc tctatttata aatttttatt aaagacttta
    80161 taaataattg tatttaatat acaaaatata tataaatatt gatgttttat atataaagtt
    80221 gtatattttt ttaaggatat aaagtaaaaa aatatacaat atcaagaaaa aaagatattg
    80281 ggtatttaaa gtaatacttt atttaatttt aagtaaatcg ggtatctaca tatatagata
    80341 ctaatttcaa agaataggag ggtgtttata tggaaatatt cgcaatagga cattcaaatt
    80401 atccatatga taagcttatt aatatgataa aaaaatatga cataaattgt gtagttgaca
    80461 ttagagaaac tccatattct aaatataata ttcaatacaa taaagaggca tttaacgaat
    80521 ctcttagaaa ttccggattt ttatatatat acatgggaaa agaatttgga gctaaaagaa
    80581 ctaataaaga tgtatatacg caagaaggtt atgcagattt tgagaaagta gcaaaagaag
    80641 atattttttt aaacggaata gaaagattaa aaaagggttg tcaaatgggt tataggatag
    80701 ttttattagg agctatgcaa gaacctataa gatgtcatag aagtatcttg gtgggaaaag
    80761 tattaaataa agaaggtttt gatgtaaaat atataatgca tgaaggaaat ttggcatatc
    80821 aggaagatat agaggaatca ttattggata agtattttag tgatagaaag caactgagta
    80881 ttgacaattt attagggagt gcattaacga gagaagaaat gatacaagaa ggttataagc
    80941 tagctaataa agaaatagga tatagaactg agaaactagg taaataaaaa tatacaaata
    81001 gataaaatac agtacttatc ttaagtaaaa taatattatg aatttataat agctaggcat
    81061 gatgtgccaa ttaaaaatat tctagttatt taataatatc attaaattta gggtattttt
    81121 taatagcaca tcatgttgca gttttaaaaa ataaaatctc taatattgcc ataatcaact
    81181 gttctattaa gagtaggatg actagatata aatctaactg taatttttga atttaacaaa
    81241 caaggatttg aaattaaaaa tactctatct ccattgacaa tagaagtaat agctactggc
    81301 aaaatatcta caaatgtatc atatctgttt ttagcaattt gataaggata atttcctaca
    81361 aaaacaaata gatttaaatc atatatatta tcagtaaacc aagcccattg accataaact
    81421 ttgtcaatat tcattgtgcc taaaccaagt gtatcaaagt atgttgctat agttaaataa
    81481 atattttcca tgttttctaa atatgaaaca gtatatcttc tgaaacgtat gggtgctgtg
    81541 gaagaaatag gataaaccat atttacattt agattatttg catttaaatt aaccaaagta
    81601 tcacatccta aaattagtct ataaaataca atatttaaat ggatgcgatt ttgttacatc
    81661 taaagtgtaa tattttatat attgaaaaag tggtaaaagt ttggatgatt aaataatatt
    81721 ataaaaaata tataaaatgt acttattgtt attgctacac gatagtgata caatattaac
    81781 gaaatatagc tacttttaat aataacggaa aatatttata ttaaattaat aaatatttta
    81841 aatgtggtat ccataaaaaa taataaacat aagataatta taaagatata ctgtgttcaa
    81901 atgatattaa acaagattta tattatttga aagcagtata ttataatgcg aacaatacaa
    81961 ttctaaactt tgcattgtca atttacagtt cgataaacta attaaaaatt aaatttatat
    82021 aaagagggaa aagaggaata gggatgttta taaggaaaaa tgttataaag ttagcaattc
    82081 ctataatgat agagcagacg tttgtcatgc ttttaggagt atttaataca atgatggcag
    82141 gtcatattgg ggaagaagct gtatctgcag ttggaatggt tgattcaata aacaatatat
    82201 ttatatctgt atttgctgca ctatctgttg gagctactgt tgttgtagct caacaaatag
    82261 gaaaaaaaga tagtgaaaag gtgaatgaaa ctattaagca ggctctagtg tctggagtaa
    82321 tattatcctt tacaataaca atattgatgt gggtttttag aactggtgta attaatttat
    82381 tttatggttc cgcagaagaa ttggtaaaat caaatgcaaa attatacata gagtttacat
    82441 tatttacgta tccatttatt gcgacacaac aaatagcaaa tggtatttta agagggtgtg
    82501 gagatgtaaa gaaacctatg tatgtgacta tgtttatgaa tatagtaaat gtaacattgg
    82561 gatatatact tatatatggt attgatttaa atcctctagg aataaatttg caaacaccat
    82621 cctatggcat aacaggtgca gctatatcta ttgctatagc tagaataatt ggatgtatag
    82681 taattataag agctttattt aaaggaaacg aatttatatc tatgggtaaa atatttccat
    82741 ttaagattga tgttgaaact caaaagtcta tatttaatat aggaatacca gctggagttg
    82801 aacaactact ttttaatgca ggtaaattga ttgttcaagt atttatagtt actatgggaa
    82861 catcagcaat tgcatccaat gcaataggaa tgtcaattgc atccataata aatgtaattg
    82921 ggaatgcttt agctttgtct gctacaacct tggtgggaca gtatatagga cgtggagata
    82981 ttaaaggagc taagagtact ttattatatt taactaaatt tgcaacaatt tgtttattta
    83041 tagtaggagc tatatttata ccaattgcac cctggatttc aagtatgtat actcaaaatg
    83101 ctagtgtaat tgacatctca acacaattaa ttcgaagtga ttgtttagct atggtaatat
    83161 ggcctatatc ctttgtatta tcagcagggc taaagggagc cggtgatact agatatacaa
    83221 tgatgactgc agtaataggt atgtggattt ttagaatttt tacaggttac gtattggggg
    83281 ttgtattaga atttggagtg ttaggaattt ggataggtat gtatactgat tggcttgtta
    83341 gagggacttt atattgttta agattaagag gaacaaagtg gctaaaacac aaagttgcat
    83401 agaatatatg aaatcacttt tagatggata actctattta gaagtgattt tttatgtagt
    83461 tattatcata attataagta tgatatataa ggtgattaaa ttacataaat aaaggctata
    83521 atagtatgga tattataaat tatatattga gcagaattac ataatttggc gataaatatt
    83581 agaaatatat atctatagaa aataatatat atgggtatat tattaaatat aaaaaatatt
    83641 aggtatttat ttaataataa aggtgggtat tgttatgata aataaaatag gaaaaataac
    83701 tctctatgta aataatcaag aggatgcaaa gttattttgg acagaaaaat tgaactttgt
    83761 agtaacttat gaaaaggaaa tgggtccaaa tatgaaatgg gtggaagtag caccaagtaa
    83821 aaatgaattt acaacatttg tgttgtatga taaaaatcta atgatgaagc aaaacaaaga
    83881 gataaatgta tctcatccat caataatttt aagtacaaga gatattaatg gaacttatga
    83941 acaaatggca aataacgagg ttgaggttgg ggaattaatg gagatgccat atggaaggat
    84001 gttttctttt aaagaccaag atggaaatga ttatttggtc agagaggata aataatttta
    84061 caaagtatta aatttactca atatctgata taattagaga caataattat tataaactag
    84121 tctaggatta gaattatata attaaaaatt aagtgtaaat tttgagtaga ggtgataaaa
    84181 tgtttaagaa aatggcagta ctaaaagata tagcaactaa aataggtcgt aaaaaagcgt
    84241 atgaactatt agaaatggtt gaaggtaatg atgcctttgt agctgaggta aagataaaaa
    84301 agaatggaat agaatctaaa aaagaagaaa ttatgttaaa agataatcaa aaaataatat
    84361 tagagtatat agaaggttaa attttaaata catataagtt gatatactaa atttctgaga
    84421 tgagtttaca taaaactcat cttttttact tttcaataac tttgttttat tatttaatat
    84481 acattttatc tccttagagt tgctgatata taaaaattac tgatataata aaagaatgga
    84541 tattaatgta atattttata gaaaagaggg gtattatgct tgaaataaaa aatctttcct
    84601 ttaatgttga gtctaacaat gaggaactag gaataataaa tgatgttagc ctttcatttg
    84661 agaggggaaa acttattgtt atcacaggtc caaatggtgg aggaaaatct acaattgcta
    84721 agcttattat gggaattgaa aaagctacga gtggtcagat aattttagat ggagaagata
    84781 taacaaatct tagtattaca gaaagagcaa aaaaaggaat aggatacgca tttcaacaac
    84841 caccaagaat aaaaggtatg acagttgaaa atttacttac acttgctcat ggaaaaccat
    84901 tgtctactga tgtatgttgc caatacctta cagatgttgg attgtgttca aaagactatt
    84961 taaatcgtga agttgataac tctctttctg gaggggaaat gaaaagaatt gagatagcaa
    85021 ctctttttgc tagagattta aaggtttcta tatttgatga acctgaagca gggatagacc
    85081 tttggagctt tggaaaactc aatgaaagtt ttaagaaaat acatgaagag tctaatcaga
    85141 ctataatcat aatttctcac caagaaagaa tattagagtt agctgatgaa ataattgttc
    85201 tacaagatgg ttcagtaaaa agccatggaa caaaagaggc tatattacct gaaatcatgt
    85261 gtcaagttaa ttcaagttgt gaattaatga aagacatgaa ttaattagaa aggaaggcaa
    85321 attaaatgaa caaaatagat ttaaatcttt tagaaaaagt tgctgaactt cgtggaactc
    85381 cattaggagc atacaatata cgtaaaaatg ggcaaggagt agagagacat tcaactgaaa
    85441 atgtaactat aattcctaaa actgataaac ctggaataga tattatagta aagccaaata
    85501 caaaaggaga aaatgtacat ataccagtaa tagtaacaga ggctggtgta caggatatgg
    85561 tatataatga ttttgaaatt ggagaaaatg cagatgttgt tataattgct gggtgtggta
    85621 tacataactg tggttgtgat gatagtgaac ataatggtat acatagattt ttcttacgta
    85681 aaggagctcg actaaaatat gtagaaaaac attatggtga aggtgatgga gaaggaaaga
    85741 gaattttaaa tccagttact attgtcaata tggatgagga ttcatattgt gaaatggaaa
    85801 caattcaaat taagggtgta gattcaacta aaagagaaat ggatgcaaac ctaggtaaag
    85861 gagctaaaat agttgtattt gagaaattat taactcatgg taatcaagtt gcagaatcta
    85921 atatgaaggt tatcttagag ggtgaaaact cttctgctca agtagtttct cgtacagttg
    85981 ggcaagataa ttctaagcaa aagtttaatc cttgtgttat tggggattcc ctttgttctg
    86041 cacatgttca atgtgattca ataattatgg gaaattctca aattagtgct atacctgaaa
    86101 ttgctgcaaa tcatccagat gctcttttag tacatgaagc agctattgga aagattgcag
    86161 gagaccaaat actaaagatg atgacattag gtcttactga agaagaagcc gagcaacaaa
    86221 tacttaattg cttcttaaag taaattaaag attttattta atttaatata attaatatag
    86281 tataatatag tgaataagtc taaaatattt ttatttttag gcttattttc tttatgtaag
    86341 aaaagctatt agagaaatta tattgtaatt aggatgtgga ataaatgaaa aatttactta
    86401 taagaaatgg gacaataata tctccaacag atggtatcaa atttgaaaaa agagatatct
    86461 tggttttaca tgggaaaata tatgctattg gattgaacct taaagagaaa ataagaaaag
    86521 aaaaattact tacaaataaa gagttcgaga taataaatgc cgataatatg tatatttccc
    86581 caggatttat agatgttcac acccattgtt ataagagaaa attacctaca ggtatggatt
    86641 ctgattcaat tggaattgaa aaaggttcta cagttatatt tgatgcaggt acagctggtc
    86701 cctcaaacta ctatgatttt aaaaaaaagt atatggatga gtgtaaaaca gaagtatatt
    86761 cttttttaaa tgttacaaat gagggactaa atatattgaa tgaatcaact tatttagatg
    86821 ttataaattt tgacaaggtt gagtcaattg tagaaaaaaa tatagaaaag ataaagggta
    86881 taaaagtaaa agcgagtaaa tttcaatcta atgaaagtgg tatagatata attaagaaat
    86941 caaagcaagt tgctaaaaaa ttaaatttac ccttagtggt atatatagga gagtatccat
    87001 catatataga tgaagtaatg aatattttgg gaaaaggaga tgtggttaca ccggtatatg
    87061 gaaatagtac aaatggaatc cttaaagaat ctggcacatt aagaaaatca gtaataaatg
    87121 caaagaatag aggagtattg tttgatgtat gtcatggagt ggaccaattt gattttaaag
    87181 tttttaaaaa agcaataaag caaggtttag agcctgacct aataagtact gatatgcata
    87241 taaaaaacat gaaagacgta aactattctc ttttaaatgt tataaataaa attatggaac
    87301 ttgggatatc cctagaaaag tgtatagaaa aagtaactga atacccaaca aagattttcg
    87361 atttacatgg aaataaaggc agaataaaga ttggtggaga aggtgatttt acaatattta
    87421 caatggagga gtgtaatgat attataaaag actacaataa taatgaactt gttttaaata
    87481 aaaaattaag attgcaatat actgtaaaat catggatgaa aagcagtgaa gtttatagac
    87541 atggatacga aaatactata ataaactaaa caatagtgat aaaataataa atatatgttt
    87601 aaattggtat atattggata aatatctaag gaaacaacaa ttaaaaaata ataattttag
    87661 gggaggtatt ttagtatgaa agcatttgtt gataaggatg tatgtgtagc ttgtggagca
    87721 tgtataggaa catgtccaga cgtttttagt atgagcgatg aagggcattc agtagcaatt
    87781 gaaggagaaa taccagcaga tttacaagat ttatgtaaag aagccgaaga aggatgtcca
    87841 gtttcagcta taacagttaa atagagctgt ttaaatttat ttttaaaata aatatataag
    87901 atttaattgg tacttaagct atagtattag aaatttctat tactatagct ttttaaaata
    87961 gataaactta atatataaga tattactttt tacaaatatg gaggactgaa atatgaaaaa
    88021 aaattatgaa atagtattat tcgatttaga tggaacactt acagacccta aagaaggaat
    88081 aacaaagtct attcagtatt ctttaaatag ctttggcata aaagaagatt tggaaaactt
    88141 agaccaattt ataggaccac cattacatga tactttcaag gagtattata aatttgaaga
    88201 taaaaaagct aaagaagctg ttgaaaagta tagagaatat tttgctgata aagggatatt
    88261 tgaaaataaa atttatgaga atatgaaaga gatattagaa atgctctata aaaatggtaa
    88321 aatattatta gtagcaactt ctaagccaac agtttttgca gaaactatac ttagatattt
    88381 tgatatagat agatatttta aatatatagc aggtagcaat cttgatggta caagagtgaa
    88441 taaaaatgaa gttatacaat atgtactaga cctatgtaat gtaaaagata aagataaagt
    88501 tattatggtt ggagatagaa agtatgacat aattggagct aaaaaaatag gtatagattc
    88561 tattggagta ttgtatgggt atggttcttt tgaagagatt agtgaaagtg aacctactta
    88621 tattgtcgaa aatgtagagt ctataaaaga tattttgtta taaatatctt ttatagacaa
    88681 aaatattaca aaatggtgtc gcatatttaa aaatatgata ttatagttaa ggaagtcatt
    88741 taaaaaagag gaggatgcac ttaaaatgaa atctatttta attatgggtg gaagcgactt
    88801 cataggttct gcacttgcta agcgcttgat taaatgtggg tatcaaattg atatacttac
    88861 aaatggtaaa aaggaaatag attataatgg ttttaaagaa catctaattt gtgatagaaa
    88921 agttcgaaaa gatatggaaa acattatcac aggaagaaaa tatgattaca tatatgatat
    88981 gacagcatat acaaaagaag atgtaagtaa tctgattgat tttataagca tggataactt
    89041 aaaaaagtat atagtattgt cagcaggagc agtatataaa gatagtggaa gaaatataaa
    89101 agaagaaaat gagaaaggtg aaaatgaaaa ctggggaaaa tatgggttaa ataaaaaaga
    89161 ggctgaagat tttattataa atagccctat accatatata attattagac caacttacat
    89221 atatggtgaa aataataatt tatatagaga gtattatttc tttgaaaaaa tagaaaaaaa
    89281 tgaaaagata ccagtaccaa aaggaaaaca agttagtaat caatttatat atattgggga
    89341 tttggtaaag gtattggaaa gcataatgaa aaatcctcat gtaagagagg cctataatgt
    89401 gacaaatcca cagttaattt cttgggatga ccttatatat acatgtggag aaattattgg
    89461 caaagaacct ataataaaat atgtagatat ggaaaaagta gagtttagag aaagaacgta
    89521 tttcccattt agaaatatag actttaatct tgatataaat aagcttattg aacatgggct
    89581 ttatatacca aacgtgttac taaaagaagg tcttacagca acatataaat ggttttcagc
    89641 aaataagcca aaaatgcatg acagaaaaat gaacaaagtt gagtctgtaa tgcaaatagt
    89701 ttaaaaaaat aaataagtag gtagatattt tgaactcaaa aattaagtat tttttaaaat
    89761 tttttatctt aatatttatt ttgattttta tatactcagt tgcgatagag ccgagtctat
    89821 taattgtgaa aaaatataat ataagtgaaa atggagcatt gataaaaaat gattcaattg
    89881 agagtaaatc tgaaagtgta aaagtagttc aaatttcaga tactcaaata ggtagttttt
    89941 attcaactaa gaatctcaaa aaagtagcta ataagattaa cactttgaat ccagatataa
    90001 tagtatttac tggtgattta attgattact ccaataaaaa tccaagtgta gatgaaatta
    90061 caactatttt atcaagtatg aatgctaggt tgggaaaatt ttcagtattt ggcaatcatg
    90121 attatatgta taaacttcct aggtattata ggcagattat gaaaaattcc aacttcaatc
    90181 ttttagtaaa tgaaaataaa aaaataaaat taaaagatga taaatatatt aatatattag
    90241 gtgttgatga aatattaaat ggaaatccta atataaaata tctggagagt caaatagata
    90301 ataaaagttt taatttattg ttagcacatg aaccagattt agtagatatg cttagtaaag
    90361 atacaatgaa tttagttcta tctgggcata gtcatggtgg tcaaataaga ttacctatta
    90421 agggagcatt agttactcct ccatatggaa ggaaatatac taaagggttt tatgacataa
    90481 atggaaatca tttatatgtg agttcgggtc ttggaagtac aaagttacca tttagatttt
    90541 ttaatatacc agagataata gaatttaata ttataatcta acataaaata tttagaaaat
    90601 ataattttgc tttttataat atgtagatgt atatactgta aaaatggatt aaaccagt
//
LOCUS       DAMJVK010000003        87132 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.003, whole genome shotgun sequence.
ACCESSION   DAMJVK010000003 DAMJVK010000000
VERSION     DAMJVK010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 87132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 87132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..87132
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.003"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            241..1215
                     /locus_tag="PC414_000199"
     CDS             241..1215
                     /locus_tag="PC414_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2462615.1"
                     /translation="MNNINSLKVIKLSLGCSLAIFIAWLLKLEYSMVAGVIVLLTVKD
                     TKRETLKGSIGKIYGFLLCTIFSYLCFNILGYNLTSFSIFIFIIIPLCFLLNIQDVIA
                     MCVVIASHYYLQGETSIKWILNEAGIFAVGTGIGVLINMYIPTNIHKIHEVQKKLQEE
                     VSIVLIDIADIIVNPKKENGYSRDLYTLNSLIDSSISETYDNINNTLLSDTRFFLEHM
                     DIIKSQRDILENLYSYVSQLNSTPPQAHILSAFIHKIGYTEFEAETGNLLLEELKRLM
                     LSMKNQPLPVDRTEFENRAILFLCLTELKQFLVNRKHAQMLRDNNFYK"
     gene            complement(1331..2392)
                     /locus_tag="PC414_000200"
     CDS             complement(1331..2392)
                     /locus_tag="PC414_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HDF2462616.1"
                     /translation="MKKIRKLATKLLITTIVLISGMSMTVYGMTAKEVTAKTPKSYVT
                     GTNSVYGPKLSQAQLNSVAQATADFMNKKITKNMTTDAKILVAYNHIKNNTTYVDWNA
                     VEGANTAYTLVTKKGACSGMARSMKALCDAMGIESYYVHSTSNDHQWNLIRFGDGRLY
                     HVDIDANKSAGKDIIYKSLSHPLPFDKTAYPKVGAIVQTVKPEQTQKPAPPGKHTMLP
                     HQEGAKAVGNFGKKGEDINEKHVMYEGVRCSVVNSSVNTITFTGSESNLDPSNLTIAV
                     IGFMNTPYGMFALAPEGKPVEYPFKYDKPFSLDFSGKNRSGAELAKYCKKNDVTIKIE
                     ITDEKANIGLGQIMYVRYE"
     gene            complement(3004..3855)
                     /locus_tag="PC414_000201"
     CDS             complement(3004..3855)
                     /locus_tag="PC414_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2462617.1"
                     /translation="MSCILKLKQIYEDLTEVDKKIADYILNNTEAISKLSVSELASNS
                     KTSTASIVRFSRKMGYSGFGDLKIEIAKDLMGKENEYTYVDENYDCNIDSVINKITNK
                     NIETINQTRLLNEKDIIKEAVEQIIKAKNVYIFGVGGSALVALDLQMKLLRINKQAFT
                     SLDSHTQLMVSSNVDKEDIAIAISYSGESKEVIKSIENAKLKGCKVICITKYSDNHLS
                     KISDLKLVVPNIEKRLREGAISSRIAMLTLIDIIYISIIQENLNEAEQKLQETKRILD
                     YLNINNA"
     gene            complement(3872..4792)
                     /locus_tag="PC414_000202"
     CDS             complement(3872..4792)
                     /locus_tag="PC414_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HDF2462618.1"
                     /translation="MYFLGVDGGGTKTTFTLVDEELNIVGTITKGTCHYNQIGFDNLT
                     KLLITGLEEVCKDAKINVEEITYAFVGLAGYGKIKEVLYALEVATKNAYSHINYTLGN
                     DVEIALAGSLNGEKGINIIAGTGSIAQALDKDGNLHRCGGWGYVLGDEGSAYYIGMAT
                     LKMFTMQSDGRCSKTKLYDLIKRHLNIENDYDIIKYVNDEIKGDRIEIAKFATICLKA
                     VIEGDNTASKIFDDAAYELSRLIIGLEHHFEQGTKIKVSYSGGVFKSGDLILEPLKKY
                     LNKTRFGIVRPVLTPDLGACLLAKKKYYSK"
     gene            complement(4902..5816)
                     /gene="murQ"
                     /locus_tag="PC414_000203"
     CDS             complement(4902..5816)
                     /gene="murQ"
                     /locus_tag="PC414_000203"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDF2462619.1"
                     /translation="MLIKTLENLVTEGRNKNTLQIDKEDTLGIIELINNEDKTVAYAV
                     EEQKESIAKAVNIIVDRMKQGGRLFYIGAGTSGRIGILDATECPPTYGVDFELVQAII
                     AGGNQAIFKAIEGAEDDKELGKQDIIDRGVTSKDVICGIAASGRTPYVIGAMEYAKEL
                     GCAVLSITMNPNSEMSKKADLPINIIVGAEVIMGSTRMKSGTAQKMVCNMLTTASMVK
                     MGKVYSNLMVDVKTSNEKLVERAKRIIMIATNVKYDVAEKFLEEADNSVKLAIFMIKS
                     GLDKDSAKSILDRQEGYISEALKSIEKL"
     gene            complement(5915..7012)
                     /locus_tag="PC414_000204"
     CDS             complement(5915..7012)
                     /locus_tag="PC414_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDF2462620.1"
                     /translation="MGRLGISIYPEKTTSKEIYDYIDKAAENGFTRIFSCLLSVKDTK
                     ENIIKKFKSINEYAKFKGFEVILDVNPRVFGELEISYKDLNFFKKTKADGIRLDMGFT
                     GSEEALMTFNPQNLKIEINMSNNTNYIDTIMDYQPNKENLIGCHNFYPHRYSGLNLEH
                     FIKCTERFNKYGLRTAVFVTSQNETTFGPWPVTDGLPTIEMHRNLPIDIQVKHIIAMN
                     NINDIIISNCYPTYEELKVLGSMRKDMVTFDVAIEENIREVEKKILFEEIHFNRGDIS
                     DSLIRSTQSRVKYKGYKFELFNTPKIIKRGDIVIESSEYGHYAGELQIALTDMKNSGK
                     SNVVGYIRKEELFILDYIKPWQKFNFKPSTK"
     gene            complement(7245..8591)
                     /locus_tag="PC414_000205"
     CDS             complement(7245..8591)
                     /locus_tag="PC414_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2462621.1"
                     /translation="MLDKFTEFLDTKLSTSMARLAEQRHLRAVRDGIVASLPLIIVGS
                     FFLILAFPPLPDSWGVTQYLKENAVTILLPYRMTFFIISVYSTFGIGYSLAKSYKLDG
                     LTGGLLATATFLLTIVPTAIEGLTPDLLKIVEKSPELLAWYNSIPQGWQMPMANLGGG
                     SMFVAIVTSIIGVEIYRFTDKSKFKITMPEAVPASVTRSFEALTPAALIIILMAIVTY
                     FLGFDWHGFIAKLVTPLVSATDTLPSVIILIFLITFFWSFGIHGVSIIGSLARPLWIQ
                     LLDANTASYAAGQAVPGIAAEPFFQWFIWIGGSGATIGLALLLAFRAKSAYGKSLGKA
                     TVIPAIFNINEPIIFGAPIVLNPILIIPFVVAPIVMATVSWFAMSANLVSRVVVTAPW
                     TLPGPVGAYLATGGDWRASVLNLLCIAISIIIYYPFVKMYDKRLLHEEKVEEQEAY"
     gene            complement(8680..8988)
                     /locus_tag="PC414_000206"
     CDS             complement(8680..8988)
                     /locus_tag="PC414_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2462622.1"
                     /translation="MRVLFVCSSGMSSAIAVNALQKEGAKNGIDIDVLAVGTQEFEDE
                     VKNGWDIAMVAPQVRHRFDYLKAFADEVSVPCALIQAQAYSPLGGPKLLKQVQELLSK
                     "
     gene            complement(9251..9649)
                     /locus_tag="PC414_000207"
     CDS             complement(9251..9649)
                     /locus_tag="PC414_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2462623.1"
                     /translation="MSNNKKKKRKKNNNYKNAKSTKSNKNTKKQMEKNQRTDYNIPTI
                     SFKSGLILIVSSLVSTVVFPLLLSIFGIDNRFGVVIGNSLITSFAVAYTRYFVETKRG
                     FSLGFFRLYILFALSFAIIGYFWIYKGIHI"
     gene            complement(9651..10811)
                     /locus_tag="PC414_000208"
     CDS             complement(9651..10811)
                     /locus_tag="PC414_000208"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HDF2462624.1"
                     /translation="MITMYSVGLMSGTSLDGVDAVLAEISGNGRNTKVKQIEFITLEI
                     PKDIKDEIRKCCIEEESSVDLICSLNFKLGYLFSKAVKSVCHKANFHIANLDFIASHG
                     QTIFHIPRSYNNFVSSTLQIGEPAVIAYETNTKVISNFRVMDIAAGGEGAPLVPYSEF
                     LLYSDNNKNLALQNIGGIGNITIIPKSCNIDDVFAFDTGPGNMIIDGVCQRLFNRKYD
                     KNGYFASKGKINEEMLKDLMSHKYLSQAPPKSTGREVFGQVYLDNMLNKYKHVDKYDL
                     IATVTMFTAKAIYYNYKNFILPKVNVDTLLIGGGGAHNLTLIGYIKELLLEVEVLTQD
                     EYGYSSDAKEALAFVILGNETLNNSFSNVISATGAKNKVILGNITPKPFGGK"
     gene            complement(11242..12261)
                     /locus_tag="PC414_000209"
     CDS             complement(11242..12261)
                     /locus_tag="PC414_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2462625.1"
                     /translation="MLVKYLKGRSKYIALPLVVVSIINIYLFAIDIFKNKYSELIYLD
                     FLVLFIIMVFFVIDYINFRNSYTNLYNCIENSGEIDSYLVDGQSFEENLIKDIIENLK
                     IKNNSDIETYKQSLKELDEYIAKWVHEIKIPISSLSIITDRLSSIEDSLDIKNQVAKI
                     NFLVNSILYSSRSNTMFEDVFINKFNLEKLIKMSIKNNSFLLIKNNVEVSLNELENDV
                     YTDSKCMSYVLDQIINNAIKYSKEVGKIEFNSKKLENGVVLSIKDFGIGINEEDISRV
                     FDKGFTGKNGRNQLYKSTGMGMYFVKKMIDSLGHEIEVCSENGSYTIFNIYFYDISDY
                     LSLDS"
     gene            complement(12255..12935)
                     /locus_tag="PC414_000210"
     CDS             complement(12255..12935)
                     /locus_tag="PC414_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2462626.1"
                     /translation="MYRILLVEDDIDLSKEIALALEKWGFKVELIDDFEVVLDEFIDK
                     KPDVVLLDVNLPLYNGFYWCEKIRAISNVPLIFLSSRDSDMDLIMGINNGADDYITKP
                     FSIEILVTKINGIIRRVYNYSDSNSILYCEDLMFDVGKGIIKHKYKDKSIELTKNEIK
                     ILTLLLKNKNRVVSRESLMMTLWDNDEFVTDNALTVNMNRLRSKVKELGFDDFIKTKK
                     GVGYIIQC"
     gene            complement(13087..14994)
                     /locus_tag="PC414_000211"
     CDS             complement(13087..14994)
                     /locus_tag="PC414_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2462627.1"
                     /translation="MSILGIAYNNFKSNIRTYLAFFISMVFSVVVLTNFELLRYGDAI
                     NVLQDENKKFTLSVLVAVIMILSVFLFFFIWYATNIFFKNRSKEIGILSFMGLDLYTI
                     GKIYFVENMLIGISSCITGIIIGIITSRFFQVVIIKLSGFNIKVSNGLSIEAILYATL
                     IFISIFIIMAVKGFITICRSSVINLINISKKQEKIPKVGIGIYILAIVSIIVIGYGYY
                     LSTNIRTGDITSVLPVIGIIIVGTYGLFKSVMPVIFDIIIKNKKILFNGNNIIAINNI
                     NYRLNKNYKTYAIIAIIITTTISTLGASVAMKRIHKNAQEQRNVYTISIVSMDKDEVN
                     KKQIENIIKDTNKIKYSVNPELTILKKTDIKEAYDTTDTIMKYSDFCNTLKVNGNDKE
                     LKEYGERLVSGNNVIELKSPQTIASIATKEAKIKLDGQLYNISRGEVKVPVLGTGLNQ
                     SIIVVNDDTYFKLRDKGQQVYFYGAKVFQEENSQVMFNNIEKNLSLKNGYIQNGYKAQ
                     GESQWMKFAYVVLVFLFLVFAIVAGSIIYMKIYSDAYEDKEKYKILLKIGTTEKDINR
                     AILKEVAMFYTLPMLSATISSYFALRLAGDLLMTDLFGIYILSLVICLIIFIIYGAVS
                     VNKFKSVVYKN"
     gene            complement(14995..15747)
                     /locus_tag="PC414_000212"
     CDS             complement(14995..15747)
                     /locus_tag="PC414_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2462628.1"
                     /translation="MKEILKIKNISKDYGIKGFKTNVLKNISLTVNEGDFISIMGPSG
                     AGKTTLLNLMSTLDKQTSGEIILDGINISKVKNNELSKLRREKIGFIFQDYNLLDNMK
                     LMDNIALPLALGKKKSKEIEAKVFSIAKKFGLENHLDKYPYQLSGGQKQRGAAARSLI
                     TNPTVIFADEPTGALDSKSAYELLESLEKINRENNATIIMITHDPLTASYSNEVYMIN
                     DGNIKCKLNKGNSRKEFYGKIMDMLASMGGEM"
     gene            complement(16063..17217)
                     /locus_tag="PC414_000213"
     CDS             complement(16063..17217)
                     /locus_tag="PC414_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2462629.1"
                     /translation="MINKAIKFKFIIFYFFSYIIFAFASTKFTPFLSKLGYSAFERGI
                     ILSSYAITNILFQLLFGVLADKYQTMKKIVLISLSVYGIASVIMFSTESATFIIYLLL
                     VALSGGLLNTCCSLYDTWIIGCGDNINNYLSFIKTFGSIGWAIGSMVASYLILRFSYF
                     GLSVAIGLIVVLLIINILLLPDIEKINKKINVTNKDIIELLKDKKYILLVCILFLLYS
                     MVVANNCTVIDKMIALNATNSQISLKWSLQSLLEIPTYLAGSYLLKKYSSLNLLKLSA
                     IMITVQFPLFALTNDSNIIIILCVFQVFSTPLILITSKRLIFEVSPKKLRSSSQLIAL
                     SIFMGGSSLIIPTVAGFLSINIGYNYTLMVLSLFGCLAYCLIVLLNYLQE"
     gene            complement(17220..17687)
                     /locus_tag="PC414_000214"
     CDS             complement(17220..17687)
                     /locus_tag="PC414_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2462630.1"
                     /translation="MFFKRKKEAITINSYIDGKIINLGEIPDKVFSTKVMGDGFAMIA
                     KDNILKSPTKGVISFIAQTNHAIGIKLQNDVQILIHIGLDSATTKKENFKLEVKQGQE
                     VNMGEKLVEIDNKLLENTLYIPLVIVENPNTVKYHLSNLNQEGNSGIKVINFD"
     gene            complement(17701..18189)
                     /locus_tag="PC414_000215"
     CDS             complement(17701..18189)
                     /locus_tag="PC414_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2462631.1"
                     /translation="MITNKIKNITAVLKDENIFLSVVMVCFLILPVLYIVYLELTSLF
                     TNISFSQLLNNDVNASLNLISNFSAVYSAYVIYNIKKNKEIKENMIAFYLVLLGQIFQ
                     IYTITIVIMFTYIYKFVGIKQIISSIKELQWGKSYKALFSSIAVCLISLFVMIIRIKL
                     IM"
     gene            complement(18203..19534)
                     /locus_tag="PC414_000216"
     CDS             complement(18203..19534)
                     /locus_tag="PC414_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HDF2462632.1"
                     /translation="MERKRQRILIAGGGSTYTAGIVTMLIESVAKFPIESIKLYDNND
                     ERQRKVAEACAIIVREKNPEIKFSYTTNPEEAFTDIDFVMAQIRVGLYALREQDEKIP
                     LKYGVVGQETCGAGGIAYGLRTIGPIIEMIDYMEKYSPNAWMLNYSNPAAIVAEACRV
                     LRPNSRIINICDMPVCLEEIFARVLGLNSRKDFDVRYYGLNHFGWWTSIKDKEGNDLM
                     PKLQEYCAKKGYEEFTPQGQHKESSWLETMRAAKDLLEIEPTTLPNTYLKYYLMADET
                     VEHANPNYTRANEIMDRREKDTFEECERIIKNGTARDTWFDASEHSQFIVELACALAF
                     NTQERFLLIVPNNGAIENFADDAMVEIPCLVGKDMVEPMSIGKIPTFQKGLMEQQVAS
                     EKLAVEAWIEKSYQKLWQSFTMSKTVPSAKVAKEILDEMIVANKDFWPTFK"
     gene            complement(19548..21113)
                     /locus_tag="PC414_000217"
     CDS             complement(19548..21113)
                     /locus_tag="PC414_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003233606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2462633.1"
                     /translation="MMKKIQRFGGAMFTPTLLFAFAGIMVGFSIVFQNQSIMGSLATP
                     ENIWYQFWGVISSGAWMVFNQLPLLFAISLPIALAKKQQARACMEALATYLTFNYFVG
                     SMLSFWGKSFGVDFAAEISAGSGLVSIAGIKTLDTGMVGALLISGIVIYIHNRFYDKE
                     LPDFIGLFRGSSLVVAICFFVMIPVALLTCFIWPHIQNVIRHLQTFFINSGNIGVWCY
                     AFLQKILIPTGLHHFVYAPICYDSVVVPGGTSVYWATHIQDFQTSAKTLKEMYPIGFS
                     LSGLSKVFGSLGVFGAFYVTAKPEKKKKVLGLMIPATLTAVLTGITEPLEFTFLFVAP
                     LLFLVHAFLDACLQTISFALGVVGDFGGGIINWVVLNWLPLGMYHWKVYVVQVVVGII
                     FSFIWFFVFTFLIKKFDMKTPGREEDSEETKLYTKNEYLETKDEKGNKLSKASQQASE
                     YIKLVGGAENVVDVTNCATRLRLTLKDDSIISKEEDFKAVGAHGLVHNGKAIQIIIGL
                     SVPSVREEFENLL"
     gene            complement(21283..22068)
                     /locus_tag="PC414_000218"
     CDS             complement(21283..22068)
                     /locus_tag="PC414_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2462634.1"
                     /translation="MRLEEMIFLNYNKLNDTDLLIWKYITANKKHCSKMSINELAMKC
                     NVSRATITRFVKKLDLKGFSEFKVLLSWESEKVHVIENQSFDIACNSIIKYVEDQKNK
                     DYDALCKLLYESRTIYAYGSGDIQNVVAKQIKRMFLSCQEIIYDFEGRTFDDSFYNIV
                     SKNDVMIIISLSGENENTLNIARRLKLIGTKIISITEFKNNTLTDLSDESIYISATNI
                     SFLQMHPSYKLTMLYFILIELLFIKYSIYKRHRMITEGIDCKL"
     gene            complement(22235..23434)
                     /locus_tag="PC414_000219"
     CDS             complement(22235..23434)
                     /locus_tag="PC414_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HDF2462635.1"
                     /translation="MKKKVLFLVIILAFIILLGNSTYSQKNQLSEEEKVDDFNYVYNV
                     VKTGYPYLGVNKRLNNIDWLANKDEYTKRIKNTENDEEFIEELSSILSDLNNKHTEVI
                     DNKKRYELFKKSYSNNDWYDFLNDKKVVDRYDSMNLKIKMPDDIFVKKELILKDVIKG
                     QIGYMYLPSMSSKNGSINNDLKIIGDYINTLEKHKALIIDIRGNLGGSDRYWQGIVSK
                     LIKNDVKINGYRIYRNNNEIVKKYTNARNIKLNPVENLPASVKENAPKEIIKDFNDFE
                     DTSYTIKSTDDSKFKGNIYLLVDRKIYSSSESFAMFCKENKFATLVGETTGGDGGCID
                     PVLFNLKNSGLIVRMASCMYLNKNGICDEEFKTIPEFKVKDCKRTADFKDDNCIRKAL
                     ELENINY"
     gene            complement(23956..25293)
                     /gene="xylA"
                     /locus_tag="PC414_000220"
     CDS             complement(23956..25293)
                     /gene="xylA"
                     /locus_tag="PC414_000220"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HDF2462636.1"
                     /translation="MSEIFKGIGQIKFEGVKSDNELAFRYYNPEQVVGNKTMKEHLRF
                     AMSYWHTLCGEGNDPFGVGTVERPWNNITDPIEIAKIKVDAGFEFMSKMGIEYFCFHD
                     RDIAPEGRDLEETNKILDEIVEYIKVNMEKTGIKLLWGTANMFGNPRFVHGASTTCNA
                     DVYAYAAAQVKKAMEITKYLGGENFVFWGGREGYETLLNTNTELEMDNFARFLQMAVD
                     YAKEIGFTGQFLIEPKPKEPTKHQYDFDTATVLGFLRKYNLDKYFKMNIEANHATLAG
                     HTFQHELNIARINNVLGSIDANQGDLLLGWDTDQFPTNIYDATLAMYEVLKQGGIAPG
                     GFNFDSKVRRASFEVEDLFLAYIAGMDTFAKGLLIAHKLLEDEVFENFTKERYASFSE
                     GIGKDIVEGKVGFKELESYALQMPVIKNKSGRQEMLEAILNRYIYEVDTISNK"
     gene            complement(25389..26939)
                     /gene="xylB"
                     /locus_tag="PC414_000221"
     CDS             complement(25389..26939)
                     /gene="xylB"
                     /locus_tag="PC414_000221"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HDF2462637.1"
                     /translation="MKLEYVLGVDIGTSGTKTVLFDKLGNTIKSCTYEYPLIQEKSGW
                     AEQDANDWWKAVVESIREVVQSSNISSECIKGIGLSGQMHGLVMLDNEGKTLRNSIIW
                     CDQRTVKECEEITDLVGEERLIEITANPALTGFTASKILWVRNNEPDIYKNTNKILLP
                     KDYIRYKLTGEYATEVSDASGMQLLDIRKRDWSDEVLEKLNIDKNLLGKVYESQEITG
                     YVTRDVSSLTGLKEGTIVVGGAGDQAAGAIGNGIVKDGVVSSTIGTSGVVFAYTKEPK
                     IDKEGRVHTFCHAIPNTWHVMGVTQGAGLSLKWFKDNFCQSEVEVSNSLGEDVYEIIN
                     NQVSKVPTGCNGLLYLPYMMGERTPHLDPYARGVFFGLSPIHSKKEIARSIMEGVSYS
                     LKDCMDIIENLNIDVNEVRASGGGGKSKVWRQMQADMFNQDVYTINSSEGPALGVAIL
                     ALVGAGIYENIQKACDAIIKTSTKLEPISENVDTYNIYHRLYKRIYKSLKDDFKLLDE
                     VVNAKINN"
     gene            27147..28307
                     /locus_tag="PC414_000222"
     CDS             27147..28307
                     /locus_tag="PC414_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HDF2462638.1"
                     /translation="MVTDKYTIREMNERLVLEQIIKNGPISRASIASTIGLNKATISA
                     ITKKLIDESLVHEIGIGNSTHSGGRKPILLVFNKCAGISLSMDIGYDYIFSSLSYLDG
                     TIINSKKLTDIQVSKDNVIQLIDEIINSYNISKIDTPYKVIGLTLAIHGITCENKVLF
                     TPYYNLNEIDLYSILSKKYDFPIHIENEANLTALAENTFSTVHNSLLSLSIHSGFGSG
                     IIINNKLYSGRNGMSGEIGHTIIMPNGKLCPCGNRGCLEQYCSEKKVFEQLSSLENIP
                     KIDSDIVKQLYYEDNHNAKKVIHEFCSYLTIAINNAITTYAPEIIYLNSQIISDIPEI
                     LQITKDMLVSSFNKGINIEISSLGSEASLYGGSAVNIKSFLNIQNLTLINEI"
     gene            complement(28463..28867)
                     /locus_tag="PC414_000223"
     CDS             complement(28463..28867)
                     /locus_tag="PC414_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2462639.1"
                     /translation="MNSIILMSHGNMAEEVLNSAKMIIGDSIDYPTVNMKQDDGIEGT
                     IEKLKKALEVYKESSEIIIMADLLGGTPCNAALLVANNDSRIKIISGLNLGMVIESAF
                     LDTKELAAKLEKIGKDNISMVEQNANLLSDDE"
     gene            complement(28888..29370)
                     /locus_tag="PC414_000224"
     CDS             complement(28888..29370)
                     /locus_tag="PC414_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2462640.1"
                     /translation="MEMKGIKNIRIDDRLIHGQVATMWSNKLGVTRLMVVNDAVANNS
                     VQKQVLRMATPAGIASSIITEETAIKNITAGKYEGQNVLLIVKSPVDLIPFVEAGFKI
                     HNINVGNMSSRKDTTVLRPNISVTEEEKEAFKRLLDEGIEITTIMTPDDKKTYLVDIL
                     "
     gene            complement(29390..30199)
                     /locus_tag="PC414_000225"
     CDS             complement(29390..30199)
                     /locus_tag="PC414_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDF2462641.1"
                     /translation="MSDKVSLNKSNEKVKKSVLWRWFFTSSVSSNYEKMQALAYCYAV
                     LPFLKVVYKDNPEKLQSAVLNHLQFFNTNPWVAPYILGINVAMEELSSEETEEAVASI
                     KTGLMGPVAGLGDSLFVVIPWTIFGAIAANMAIDGSPFGILLWIVVSVALKLLSIPLF
                     NAGYTSGTKLISSIEKSLKILTESTSILGLMVVGALIPSVVKANVALNFQQGDFTMKG
                     QEILDQIMPGLVPAILVVIVYWALKKNIKPIYLILGVMVVSIILYALGILK"
     gene            complement(30192..30971)
                     /locus_tag="PC414_000226"
     CDS             complement(30192..30971)
                     /locus_tag="PC414_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2462642.1"
                     /translation="MEPQLWQLLLIVLYGFFINYEKNSTMFGTYQPVTAGFITGLILG
                     DINTGLYIGGTLQLLSLGISNFGGASIPDYQTASIVATFITITTKQEASVGISIGIPV
                     ALLMVQLDVLRNTIGIWLVHKAEDGAKKGNYKNITYMQMLGVLLTAATTGIPVALSVI
                     FGPSLINTILKYTPEWLTGGLTVAGGLLPAVGIGLLLRYLPAKEYFSYLVIGFVLAVY
                     MKVPLLGVALIGGAIALIIYKKNLENQEQQYTVVGGMDEDE"
     gene            complement(30971..33049)
                     /locus_tag="PC414_000227"
     CDS             complement(30971..33049)
                     /locus_tag="PC414_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HDF2462643.1"
                     /translation="MIKISEDYIEKRFDNELLRIEAWGKNSLRIRSFVDQNFVDENYA
                     LNEKPKLNKDDITINKNEDGSAIIKNGKIKAVLDHRDRITFYNDKNEILLKEYIRLRA
                     VKHDDGGEDVGTIEITKDFNSTLKLKSREYRPSYSGEFEVTTRFESEPSEKIFGMGQY
                     QHKFLDLKNTVLELAQRNSQISVPFYISSLGYGLLWNNPGIGKVSFAKNMTEWKMFST
                     NFIDYWITCGESPKELNKNYSQVTGTVPMMPENLLGLWQSKLRYRTSEEVLDVVKEYS
                     KRGIKLSSIAIDYFHWPKQGEYKFDLDYWKNPKELVKKLKEEYSVEPIVSVWPTVQSD
                     AENYNDYLENGYLVNVNRGVRMTMQIQGNTVFVDMTNENAREYVWDRIDKNYKQLGID
                     YYWLDVAEPGYSVYDFDNYRYKKGNVLSCGNIYPIDYLKMIYDGLHNDIESVVTLVRG
                     AWAGAQKYGALVWSGDIDSSFEAFNNQVNTGLNMGLAGIPWWTTDIGGFHGGNPKDPE
                     FRELMVRWFQYATFSPILRMHGDRLPHSKPLSNKGGGSMVTGAPNEIWSYGEEVEVIL
                     TKFIKIRESLKTYLKKLMKEAHEDGTPVMRTLFYEFPEDDKTWEVDNTYMLGDEILVA
                     PIMNYKDRSRKVYLPKGHTWENIFSGVSYEGGKTYEVECPLEEIPIFLKQDSSYNFKE
                     LKNILGEVKS"
     gene            complement(33848..34369)
                     /locus_tag="PC414_000228"
     CDS             complement(33848..34369)
                     /locus_tag="PC414_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HDF2462644.1"
                     /translation="MKRNREIVVVCHCILNCNSKVEGLSEFKGAQNIAINLINKGYGI
                     IQLPCPEMIMYGINRWGHVKEQFDNLFFREQCRQMLIPHVRQFDEYLKNDYDIKSIIA
                     IDGSPSCGYNKTCSSKRWFGELSGCNELMDKIKDIQMVDGKGIFIEELEKLLKEYNLE
                     IPIVGFDELREDK"
     gene            34535..35101
                     /locus_tag="PC414_000229"
     CDS             34535..35101
                     /locus_tag="PC414_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDF2462645.1"
                     /translation="MDTKKVTFSSMCIVINIVLGMVISMINIPLLFLDTVGTVLGAVV
                     LGPFWGALIGGCTNLVLGVISGPTNIPFALVNIVLGLIVGYISKKKGFGYKEAIITGV
                     ILSIVCPLIGTPISVLLFGGLSGSGADLLVGFLVQSGQKIFTAAFFPRILSNIVDKPL
                     SCIMVVYFISKMPKGFIKQYSKNTSSSY"
     gene            complement(35318..37381)
                     /locus_tag="PC414_000230"
     CDS             complement(35318..37381)
                     /locus_tag="PC414_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HDF2462646.1"
                     /translation="MKAGEIEFLSYLEGSNKSFVVPVYQRNYNWKKEQCKRLFDDLED
                     IIDSGFRTHFLGSVVSIYSMGKEYLIIDGQQRVTTVSILLIAMCQIIKEFGESKGIIT
                     TEEQIKECYLINKHSRDKDGKIKLKPIAEDRKAYYELFEEEMNTDRNSNIVSNYLYFY
                     NRIKEDNISIDDLFDAIQKLTIVEIELKNGEDDPQLIFESLNSTGLDLSEADRVRNFV
                     LMGRSSKLQEEFYNNYWYKIEKNTSSNVSSFIRDYLTIKERSIPNKNKVYFSFKRYVS
                     DNGIDIEILLKDLLKFSNYYREISYSCTDDNDVNKVLKRINKLEIFVSYPFILELFDD
                     YNEGIINKKVLVESLNLVECFILRRIVCDVPPNALNKVFMNLGREIKKFSDYKENYSN
                     ILRYIFINKKSSQRFPTDSEFSNAFETKDIYNTKSKNKLYILERLENYSNKEIVDLEN
                     LINNNMLNIEHIMPQTLTNKWKESLGDNYKEIHDKYLHTIGNLTLTGYNSKLSNKTFE
                     EKKEMEDGFKDSRLYLNKYISSIDKWNEEEIKNRTKILLDRAVEIWKYPSVTYEPPSN
                     SENIFTLEDDDVNFTNESIVSFSILGESYKAKNWTEFYEQVALTLYDLDSVRFNSIVD
                     KTYKRDFIDNIKSRDAYKLRKGIKISENIYLETNLNTEAKLNIIRFLLNEFDIELNEV
                     IFSIK"
     gene            complement(37762..41097)
                     /locus_tag="PC414_000231"
     CDS             complement(37762..41097)
                     /locus_tag="PC414_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDF2462647.1"
                     /translation="MGNFDFLQKKKNFNSFNTACLEAEKSILVSPSTCATITRRALEL
                     AVKWLYANDSDLVLPYQDNLSTLIHNNSFIELIDYDMLPLLKYIVKLGNLSVHTSANI
                     ERGEAILSLNNLHQFVSWIDYCYSDEYTAEDFNEDLLLHGEEKRTRPDELKNLYERLS
                     SKDKKLEEVIKENEELRKSLTEKRKVNTVNYDFKIDEVSEFETRKRYINIELKLAGWE
                     FGKDIGEEIEVQGMPNESGVGYVDYVLYGENGKPLAVVEAKRTSKDPKIGQQQAKLYA
                     DCLEKRYSQRPVIFLTNGFDMYIWDDYSDRKVYGFYKKSELQLMMDRRKSKKSLSSVT
                     INDEISNRYYQKEAIRAVCEALENKQRKTLLVCATGTGKTRIAISIVDVLSRHNWIKN
                     ILFLADRKALVKQAKKSFTKLLPNLALCNLLDSKDSPEDARMIFSTYPTMMNAIDDTK
                     SKDGKRLFTPGHFDLIIIDESHRSIYKKYKSIFDYFDSYLMGLTATPKDEIDKNTYSV
                     FDMENGVPTYAYEYNKAVEDGYLVDYTSIEFKTKIMEDGIKYDELSDEEKEEYENTFN
                     DDESIGDEIGNNAVNEWLFNSDTIDLVLNKLMTEGLKIEGEEKIGKTIIFAKNTKHAR
                     AIVERFNKLYPKYGGNFVKAVDYSINYVDSIIDDFSDKNKLPQIAVSVDMLDTGIDIP
                     EILNLVFFKKVRSKTKFWQMIGRGTRLCPDLLGVDMDKERFLIFDFCNNFEFFKFNPK
                     GFEGNKAETLTEKLFNIKVSMVKELQDIKYIEGEYAELRKELLEELITSVKALNEDSY
                     IVRMNLSYVHKYKNENVWSNIGAVAQNEIREYISPLITSYSDDELAKRFDVVMYNIQL
                     AYLQNNNASKGIRHVMATAEKLSKLGTIPQIQQQKYTIEKAMTENFWEDSDIFEVEEV
                     RISLRELIKYLEKSSQKIYYTSFEDMIVAEDRNDSVYNANNLKNYKKKVEYYLNSHKD
                     ELAIFKLRNNKKITKQDVETLEEILLKQLGNCDDYKKEFGDTPVSQLVRRLVGLDREA
                     ANEAFSEFLNNKSFNTKQIHFVKLIVDYVVKNGFIEDNKVLMEDPFRTVGSIIDLFEN
                     HIEERNKLIKTINKIKENASEIG"
     gene            complement(41147..41575)
                     /locus_tag="PC414_000232"
     CDS             complement(41147..41575)
                     /locus_tag="PC414_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2462648.1"
                     /translation="MVIRNKLSWNSIEEVNRDFGSCLYDLQNFKILYDKEEMPELWAR
                     YIKEGFKSYMVSFLELTKAMLYYKSIDLNIKSKNFYDYLLACEYHNLLPKNSSIVIET
                     LRKLRNDDSHGYDIPQFEDMYELFTENEEVFVSIRNSCKK"
     gene            complement(41565..41945)
                     /locus_tag="PC414_000233"
     CDS             complement(41565..41945)
                     /locus_tag="PC414_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HDF2462649.1"
                     /translation="MRNIPNEIKNSIINVFPKENTLAIIIFGSYGTDKHTSESDIDIA
                     WIPIKKVPITELATKTEALRNILDIDVDLKIVTDNYTIALKKAILEGEVIYESKDFKD
                     YINKFYFENSDVIDILDWRDMYGN"
     gene            complement(41978..43150)
                     /locus_tag="PC414_000234"
     CDS             complement(41978..43150)
                     /locus_tag="PC414_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDF2462650.1"
                     /translation="MEYIKLNELCYINIGKTPSRNTSDYWGSGNRWLSISDLKEKYIS
                     KSKEEITDLAVEKANMKLVPKNTVVMSFKLSIGRVAILKEDMFTNEAIANFQIKNNEL
                     ITSEYLYYALRTLNFNNTDRAVMGATLNKSKLNDIKIPYFTICIQNKMVEVLNKAQEL
                     INKRKEQIEALDELVKSRFIEMFGEPINNTKKFDMKKIKEISIYLKRGVSPKYVEQSN
                     IKVINQKCIYWRNLKVENCKYYDENLKEKIEDIFLKQNDILINSTGTGTLGRCVKFNK
                     IDNSEYIADSHVTVLRSISNNLNSDYFAALFEFTNIQDTIYRKCVNGSTNQIELSVSK
                     LGEYLIMVPPIEFQNQFSEFVKQVDKLKLKIETNLKELEDNFNSLMQKAFKGELFN"
     gene            complement(43154..44617)
                     /locus_tag="PC414_000235"
     CDS             complement(43154..44617)
                     /locus_tag="PC414_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HDF2462651.1"
                     /translation="MITGELRSKVDKIWETFWTGGLTNPLEVIEQFTYLLFIKGLDEV
                     ETKNEAEATLLGVSFERIFDDEHQHLRWSKFSNEGNSEKMYEIVQNEVFPFIKKLHGN
                     KDSAYAKYMGDAIFKIPTPLMLSKIVDGISNLELSKDRDTKGDLYEYLLSKVATAGTN
                     GQFRTPRHIIDMIVRLIKPTPEDIIVDPAAGSAGFLVSSQQYLRDNHSSLFLVQGLKE
                     HFNNGMFYGFDMDRTMLRIGAMNMMLHGVDNPNIEYKDSLSEVNTDKDKFTLVLANPP
                     FKGSLDYEAVSADLLKVTKTKKTELLFLALFLRVLKIGGRCACIVPDGVLFGSTGGHK
                     SIRKEIVDNHKLEAIISMPSGVFKPYAGVSTAIMIFTKTGTGGTDKVWFYDMKADGYS
                     LDDKRNEIKDSDIEDIIKRFDNLDGEVDRKRTEQSFFVDVEEIRENGYDLSINKYKEV
                     VYEEVVYDAPSVIIGRVRELEKEIAVGLEELERMLEV"
     gene            complement(45127..45996)
                     /gene="gatY"
                     /locus_tag="PC414_000236"
     CDS             complement(45127..45996)
                     /gene="gatY"
                     /locus_tag="PC414_000236"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HDF2462652.1"
                     /translation="MALVTTKQILLDAQEGHYAVGAFNVENMEMVMAVIEAAEELKSP
                     VILQTTPSTIKYAGLDYYLANVKVASEKASVPVAMHLDHGSSFGLAMQALRVGYTSIM
                     IDGSHESYEDNISISKSVVDACMPSDIPVEAELGKVGGKEDDLDGGDGGAYTDPLEAK
                     EFVERTGVSSLAVAIGTAHGLYKGEPKLDLDRLSEIRDVVSVPLVLHGGSGIPDEVIK
                     ESIKRGICKVNYATELRIAYSNGVKDVLNSDPEVIDPKKYGKKGLESVKDFVKSRMEV
                     CGCVGKAIEIVKC"
     gene            complement(46016..47932)
                     /locus_tag="PC414_000237"
     CDS             complement(46016..47932)
                     /locus_tag="PC414_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HDF2462653.1"
                     /translation="MGKKMILGVTGCPTGIAHTFMAEEALKNSARELGCDIKVETNGA
                     IGVENKLTAKDIEMADAIIVACDKNVDMDRFNGKPVIEVPVKEGIHKANELIQRCIDG
                     KVAIRKGNLSSSKIAEEGNLSFGQKLYKDLMNGVSHMLPLVVAGGVLTAISFLWGIYS
                     FDPNSEQYNQIAATLKSVGGYSMNLMVPVLAAFIAQSISGRAGMLAGLVGGMISFDTG
                     SGFLGGIISGFLAGYLVKLFVHLLRKLPRQLEGLKSIFIIPIVSVGLVGVAMLLLGGP
                     CSALNNAMMNFLSGLQNSSPIILGIVIGCMSAFDMGGPVNKAAYVTGTMLLGQGNYLF
                     MAGVSAACITPPLVIAIASTLFKNRFTEEDRAAGLINYILGSTHITEGAIPFAAKNPL
                     KVLPVLMIASSISSILTYIMKIEVPAPHGGFLVLGLVNKPLLWVGCIFVGSLVGAVLY
                     MMVTPKVVNEKNTIENTNVTKDEHKNESAESENVSNNISLYSEETVILDVKGKNKTDV
                     IDEMIGILDKSSVLLDKKKFKEEILKREEISSTGFGMGIAIPHAKTDAVKVPRVAVGV
                     SKEGFDFESEDGSLAHIIFMIAATDNGDNLHLKTLSQLSSKLMNEEFLNELLNSKTSR
                     EIVAKLNNEEIKSV"
     gene            complement(47935..48855)
                     /locus_tag="PC414_000238"
     CDS             complement(47935..48855)
                     /locus_tag="PC414_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HDF2462654.1"
                     /translation="MIYTLTTNPAIDMNISTNGIKRKLVNRTSNAVYSPNGKGLNVTF
                     VLGHYGIESKILGFFGGFSGKYIVEESEKRGFDVLPTWVEDTTRINIFLNDGSDEFKF
                     VNSGSYVNEKQKLDMLDKIQSLADISYLSISGSLPPGIDDNYYEDIFKICKNKSIKTI
                     LDISSPKLKELLQYNPYLIKPNDEEIKDIFGIIVRDEEDIKDVLKLLHLKGAQNILLT
                     LGEKGSYFYNGKSIYYASAQPVTVVSSACAGDSALAAFLSIWLENPEHIEEALKRSAA
                     TGASVAESNGIGNLENVEDYIKNIRVRKVV"
     gene            complement(48957..49709)
                     /locus_tag="PC414_000239"
     CDS             complement(48957..49709)
                     /locus_tag="PC414_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2462655.1"
                     /translation="MIIELNKSNSENLTKTELEIIKFINENESILPELSIVEIAHETY
                     SSPATVSRAIRKCGLNGFNELRYRLTVKDENDDIQNMGDIINKSFIEAQRVIEQISLT
                     TLINIIKTINKSSKIYILARGLTEYVAKEFSLKLQLLDFNVVFINDPNIMKIKSKSIK
                     KDELIIIFSLNGNTIELVESAQNANLVGAKVITCCCSESAKLIKYSDLYIVGYKHEHI
                     SISEYEVSSRLPLYIISRIIIDYMVRYNNSEN"
     gene            complement(50101..51546)
                     /locus_tag="PC414_000240"
     CDS             complement(50101..51546)
                     /locus_tag="PC414_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDF2462656.1"
                     /translation="MIKLGKFPKGFLWGGAVAANQLEGAWNEGGKGWCLADVHMYDKD
                     RDISKPYESDMTIERIKFAMKDNNGYYPKRSGIDFYHTYKEDLKLLKELGLKCFRFSI
                     NWARIFPNGDDLEPNEEGLKFYDNLINEVIDNGMEPIVTLLHYETPLNLVLKYKGWSN
                     RKVVDFFVRYAELILNRYKNKVKYWIVINQINLIHYESFNSVAFCVDQVDNVEETRYQ
                     AIHHQFIATALTKKLGKEINSDMMIGMMMADCTSYPEDCDPENVVFALKRNRLQYFFT
                     DVSFRGEYPVYIKRFFKENNINIVMEEGDDKILKENTMDFLALSYYYSTTVSASKNTM
                     NPTDNIKNPYLKANPWGWAIDPKGLYNTLSQYYDRYQKPIMIAENGFGMHDKFEDGKV
                     HDSYRIDYLGKHIEQIGEAIADGTEILAYCMWSPIDIVSCSSQEMEKRYGFIYVDIDN
                     NGNGSKERYKKDSFYWYQNVIRTNGEELREK"
     gene            complement(51568..51921)
                     /locus_tag="PC414_000241"
     CDS             complement(51568..51921)
                     /locus_tag="PC414_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF2462657.1"
                     /translation="MTIEEHIIDEELVEVAMQIILRAGEARTEIKHALNDLEKFDYRN
                     ADLKLAKAKGFMTEAHRAQTKIIQGEAGGEKRAHSLLFAHAQDTLMTIFSELNITMSL
                     VGIVKSIEYKVNNSK"
     gene            complement(51950..53197)
                     /locus_tag="PC414_000242"
     CDS             complement(51950..53197)
                     /locus_tag="PC414_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2462658.1"
                     /translation="MGRVFMNKFIDFLSEKFTPVVNNMTKNIWVQSVQSTIMKVLPMV
                     FVGSLVTIVSVLKNYISFLPDLSPINQYTFGLLGLFIAFLLPMETMKNKKFESMSVVA
                     GLAGAGLMLMMIRPEITNEGAIFNFNRFGGEGMLVSLVAGLFSGLIMSLFGSFSFFGE
                     DSALPDFVSKWFDNMLPITVIMFVGWGLIYGLNFDMFEIIVRVFKPVSDIAQTLPGFV
                     LLNFLVVFIYSLGISGWVLSPITYSIQLGAIAANAAAVASGGVATNIYVYEVIQTGWI
                     TLGGLGATLPLVIMMCLSRVGRIKAIGRACIVPSIMNINEPVIFGAPVAWNPFLMLPM
                     WIGTVVISITTYLSMSMGFVTIPSKVFNLWYVPFGISTFMVNQDVRGLIVLAINIVLL
                     FMIWYPFFKAYEKEEIKLESQEV"
     gene            complement(53205..53522)
                     /locus_tag="PC414_000243"
     CDS             complement(53205..53522)
                     /locus_tag="PC414_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2462659.1"
                     /translation="MKNIRVLLACGSGASTGFMATSMRKSAKKKGIEADIKARSESEI
                     DKYLSEIDCLLLGPHLDYMKEEIQQKANEYNVPVACISKTAYGRLDGAKALEEALELI
                     SNK"
     gene            53764..55677
                     /locus_tag="PC414_000244"
     CDS             53764..55677
                     /locus_tag="PC414_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2462660.1"
                     /translation="MKTSKKELFLNFIKTQHDWIDSSTLANYLNVSTRSIRKYVNEIN
                     SNGAFILSSKKGYKVNLNTNCQTKIDSSENISPDNRLNLILKELIVNSNGINIFDLSE
                     ELFVSPATIEGDIVKANKFIGSYNLKIKQSKFLLKLVGNESAKRKLMSSIIFKETGSD
                     FLSLFDVQKIYQEYNLTKLKENIIYILKKYNLFINEYAINNILLHLMITIDRIKKNNY
                     IDSVEVVNYIDNNVDINIAKDISNFLESEYNITLTSAELYYLVFQLTNKTTVLNYNQM
                     DTKSLSNYIDEHFVKLTKKIIKNVYDLYLIDLSDEEFVVKFTLHVKNLISRAKNNQVL
                     RNQIPQKLKDSYPLIYDISVYICNQIQTLENVDIDEDEISYISLHVGSFFDRQKLLED
                     KVLCALITPNYYDLQFKIVRDLEKRFNESIEIIQIFSDTHNLDFDNKVDMVITTLPIN
                     DRCPIPFVYVNPYLNRKDYDNIQSKFIQIKDRKNILTVQNHLEMYFSESLFMKNIYLD
                     SAKDYIRFMGNILYENKYVKPNYIDDVLIREKMSSTAFNNNVAIPHSMKMDALKTGVC
                     LIVNDKPVKWGEEKVQIIAMIPINEKEKEKFNYIFESFIEILSEWNNVKELTKADNYS
                     SFMNRIAYLIQNI"
     gene            complement(55800..56954)
                     /locus_tag="PC414_000245"
     CDS             complement(55800..56954)
                     /locus_tag="PC414_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDF2462661.1"
                     /translation="MKILISIDSLKGSLSSIEAGNAIKKGILKVKEDAQVKILPLADG
                     GEGTVDALVQGMNGKKETIEVTGPIAKKVDATYGLLKNTSTAIIEIAQASGLTLVPTE
                     LRNPLYTTTYGVGEIIKEAINKGYRNFIVGIGGSATNDAGIGMLQALGFEFYDKNNKL
                     VGLGGKVLNEIRHIKIENRLKELDECKFKIACDVNNPLFGKNGAAYIYGSQKGATSEI
                     IEKLDNGLRNFSKVVKNYLSKDVANVEGAGAAGGLGFAFLAFLDSKLESGIKIILEEI
                     KLEEELKDADFVITGEGRLDNQTAMGKAPIGVAKLAKKYGVKVIGLAGATTEDAVKCN
                     EEGIDAYFSIVNRAMTIEEAMDKATASENMTATTTQIFNLITSIQQSKNM"
     gene            complement(57233..59848)
                     /gene="mngB"
                     /locus_tag="PC414_000246"
     CDS             complement(57233..59848)
                     /gene="mngB"
                     /locus_tag="PC414_000246"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HDF2462662.1"
                     /translation="MKKNVHIVPHMHWDREWYFSTEESRILLVNNMEEIMDMLENNHN
                     YPYYVMDGQTAILEDYLAVKPECKERIKKLVQEGRLIIGPWYTQTDEMVVGGESILRN
                     LLYGIKDCDEFGEYMKIGYLPDSFGQSAQMPQILNGFDIKYSMFWRGCSERKGTNKTE
                     FNWKSDDGSSVLVQILPLGYAIGKYLPMNEDELRTRMDKYLPVLDKGATTDHIILPNG
                     HDQMPIQKNIFEVIYKLKECYPDRKFFLSRYENIFKELEKNEDIDTIKGEFLDGKYMR
                     VHRSIFSTRMDIKSANARIESKITNILEPLASIAYSLGFEYHHGLIELIWKEIMKNHA
                     HDSIGCCCSDKVHQEIMNRFFLAEEKVDQLITFYKRRIVDAMSCEMALDKLIAFNLMP
                     YDRNEIVSAQVITKMKAFEIFDKDNNKLDFEVVHKEVIDAGLIDRQIVHYGNYDSFMV
                     YTINFKDKIPAMGYKAYMIKEVEHMLEKAYEVCDMVDNDFYTIQVNENGTLKIFDKKL
                     NKTFENVLLMENGGDEGDEYDFSPLPNEKLIFNDNIVANSTIKKNRFNNEIKINYRLK
                     VPKDIQARKNNRADSFIDFDIVINVPNDKAIIDVVFNINNQARDCRVRTYIPTNIASK
                     FSVSDNQFGHIKRSVYDDAMEVWQQEDWSERPDSIYPMLTFVGLSDKEHGVAVLTNST
                     REFEIVGEKFDTIAITLFRSVGFLGKEEMARRPGRPSGIKLPTPDSQMIGDIKIDLAI
                     VTHEKSTLEANVANMAKEYLTKIQTYNKMPYNAMKLNQSEIEVDYSYSLLKETNKDLV
                     LSVVKKAEKEEALVVRFYNSKEETKSANIVFNKNIKTAKWINLNEKELESISIDNNSV
                     NVECLMNQVKTILVK"
     gene            complement(59992..61917)
                     /gene="mngA"
                     /locus_tag="PC414_000247"
     CDS             complement(59992..61917)
                     /gene="mngA"
                     /locus_tag="PC414_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HDF2462663.1"
                     /translation="MEFKKITNENLVFLKQKIKTKEDAIKFLARKLYDEGKLTSYEEY
                     LEAVMYRETLSPTGFERGLAIPHGKSKSVKEAAFAAMTLETPIENWESVDPNNKVELI
                     FLIAVPESEGGNTHIQILSELVTRLSNEDYMNMLLNSKTKKELFNNLDIKEKKEQIKE
                     EIRIQNESKKIILAITACPAGIAHTYMSAEALIKAGKEIGVDVYVEKQGANGMVDPHT
                     PDIIKRADAIIYATDVAPKNTERFEHLPNIKTSVAAPLKRAKEIIYEALEVAQKQGKG
                     DYIEKSSDISYCEKSSWKKDVKSAILTGVSHIIPLIVAGGMILAFAVLASQAFGLQEL
                     YNQEGSWLWQLRKLSGGMLGTLMVPVLSAYMSYSLAEKPGLGPGFAAGLAADLIGSGF
                     LGGMLGGLLAGYIVKFLKKNIPAKGTLAGFVSFWVYPVVGSLVVGLLMLFVVGKPVAA
                     LNNGLINWLNGMTGVNAILLGAIIGAMVSFDLGGPINKAAYTFAIGAIASGNFIPYAI
                     FASVKMVSAFGVTIATVLGRDLFSESEKEIGKQTWILGLAGITEGAIPFMIEDPIRVI
                     PSLVAGSAITGSIVAFFNIGLQVPGAGIFSLALLEGSMSGFIIASIWFFAAVLGAIIS
                     AILLIITRKQKNQRKIH"
     gene            complement(62047..62772)
                     /locus_tag="PC414_000248"
     CDS             complement(62047..62772)
                     /locus_tag="PC414_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2462664.1"
                     /translation="MEFYERAKKYEYKFTDTEDMIIEYMRKNKEEIVKLSIQSLADKF
                     FTVPNTIVRLCKKMEYDGFSQLKNNLKEELKNEDKEPKNGFESDIKKTLELIDYEKIN
                     KVADIIQKSRSIIVYGIGQNIPLCEILVKEIRGGHDNITYYSQRHEVLHDIGKLGEQD
                     MFFALSLSGETKEVIEAAKTAVNKGCKVVALTHICENQLQQIADISLYCYSTIKQVNG
                     YNISDKVPIMMIMRAIIEQYWLR"
     gene            complement(63035..63814)
                     /locus_tag="PC414_000249"
     CDS             complement(63035..63814)
                     /locus_tag="PC414_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HDF2462665.1"
                     /translation="MHKMKKLVVRADDIGYSEGVTLGIISSYKNGIVTSAGIMVNMPF
                     SRQAIEMVKNDDVCFGLHVNLVIGEPCCKEHIKIKNLLDEGGKFVSSKIRRTQLLTGK
                     EVFNYEEVYEEVCAQIETYKLYMSKLPEYIDIHGIEEDVSISAMCDAAKEYGIPSCPY
                     YLDSCISMPSKYPQNDFYKLNKSFVKLFEEKYINLDKDITLMVTHPGFIDYSLIKSSS
                     LIQERVYEHAMLCDKEVKMWLKRNNIELVDFKDIFKSNNYI"
     gene            complement(63807..65234)
                     /locus_tag="PC414_000250"
     CDS             complement(63807..65234)
                     /locus_tag="PC414_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2462666.1"
                     /translation="MMQQLSLLGKTMLVPIAVMPAAGILGLLFGPNMLNVPAISNISN
                     IVFSNVDYLFLLGAASAYSKSKDKTSVMVAAVVAYMIFKSNLELLNDTLNAGVFGGII
                     VGACIAWTYNKTYKVKVPAFLSFFAGEKCVITLSPVVALILSYIFSLLWIYPQAAMNQ
                     FGFWLGGAGAIGIFLFLFCNRALIPTGLHQVLNAYILFEMGEYTTSTGEVIRGEIPRF
                     MAGDPNAGFFWTGFYIIMMFSIPAISYAIYKTAQDKKKQEVKGAMTAGALTSFMATVT
                     EPIEFSFLFASPKLYIIHSFYSGLGGVVLYLLGARLGSFNGASIIDYVLSFSYGDKAW
                     LVIPVGVVFFVLYYVTFKYIIIKDNVQTPGREVEIEIGDKVSEKEKNLKLSHGNFSYM
                     AKQIIKNCGGYENIVTLNNCMTRLRLEVKDATILNDDNIKKTGAKGVIKLSNTSVQII
                     IGTDVVKVKDEMEMQLDELRKQADA"
     gene            65625..66347
                     /locus_tag="PC414_000251"
     CDS             65625..66347
                     /locus_tag="PC414_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2462667.1"
                     /translation="MAKNKFSEIYEVLKEEILDGKYTSNMMLPTELQLIERFSCSRNT
                     VRRAISQLNTEGYVQSIKGKGVVVLENSCSNDFFLNMHNFKGVESIVEDKKVNTATSV
                     LHFSKILIDNKLSKKTGFKVGSEVYYLHRLRYIDNIPKILDINYFLCSIVKDLDVSIA
                     QGSIYKYIEECLGTKIVSSRKIFKIEKATELELKTLPLNDYNCVGVIKNSVYTDDGKL
                     FEYTESKHTPETFVFMDVTQRY"
     gene            66544..68211
                     /locus_tag="PC414_000252"
     CDS             66544..68211
                     /locus_tag="PC414_000252"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HDF2462668.1"
                     /translation="MKNWWKKATVYQIYPKSFKDSNNDGIGDINGIIEKLDYLYSLGV
                     DLLWLTPMYVSPQRDNGYDIEDYYNIDPKYGTMSDFEKLLKEAHKRDIKIMMDMVLNH
                     TSTEHKWFKESKKSKDNPYRDYYFWKDAKPDGSVPNNWISRFSGTAWKYDETTNQYYL
                     HLFEETQADLNWENEKVREECYKILEFWADKGIDGFRLDVVNLLSKTPGLPDDPITGP
                     KGDGRTHYADGPRIHEYLHNMNQKVFKPKNIVTVGEMSSTTPEECINYTRENREELSM
                     VFSFHHMKTDYKGGAKWTNEMFSLDKLKKAQSDFQYKMYEGKGWNALFYSNHDQPRAL
                     SRFGDDRFFRQESAKMLAITLFGLQGTTYIYQGEEIGMTNAYFTKIDEYDDAESKNAY
                     YHMIKSGIDEKEALLILQQKSRDNARTPMQWDNTIYKGFSNHKPWLKVNNDNISVENE
                     LNDSRSVFYTYQRLIKYRKQYDIFTEGTYRLLDENHKNLFVYEREYKNQNLLVISNFT
                     HDNVVYKLPETYLNKLNYTILETNYTRDIIENKMEIKPYESIMIYYK"
     gene            complement(68307..69626)
                     /locus_tag="PC414_000253"
     CDS             complement(68307..69626)
                     /locus_tag="PC414_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF2462669.1"
                     /translation="MEKGIKLGFWSIVLLGINAIIGSGIFGLPGDAYTDIGPASILVL
                     VFCMLLAVSIALCFAEAGSWFDTDGGPYLYAKEAFGDFVGFEVGFMKWIVSMIAWATM
                     ANFFAVTLSSVWPQAAEPLTKNIIIGILVVGLGIINFMGMKQSKHLNNIMTIGKLLPI
                     VLFIAVGLFFIKGSNFTPFVIIQKGQSASSAFVAVSITLFYAFTGFESLAVAAKDMEN
                     PKKNVPKALVMVMFVVSVIYMLILGISIGVLGNGLAGSATPVADAAIKMLGPIGGYII
                     TIGTIVSVGGINIASSIFTPRSAAALVEQGLMPKSIRKTNKNGAPYIAIIVSVIGTLL
                     IAWSGSFTTLSQISVVSRFIQYIPTCLAVLILRKKYAGKDVNFRIPGGAIIPIFAVII
                     SILLLIKAGIDEPMKIVWGLGGMIIVVPIYFYMTKVYSKKYNDVEVK"
     gene            complement(69667..70413)
                     /locus_tag="PC414_000254"
     CDS             complement(69667..70413)
                     /locus_tag="PC414_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HDF2462670.1"
                     /translation="MKKLAKKKYPIIGISGNLLIDEGGMFPGYERAYVNNDYIQSVVM
                     CKAIPYIVPIVYDDEIIKEQVSNIDALILSGGQDVNPLIWKEEPHNKLGAISPKRDSF
                     DMKLLKHALDMKKPVLGICRGEQIINVTEGGSLYQDLSLIEGAYIKHNQQHLSNVPTH
                     TVQIKEGTKLYEILGEKEVLVNSFHHLVVNKVAPGYIVSATSKDGLIEAIEKEGSEFV
                     IGIQWHPEMMTRDYDNMKKIFMAIVKEASK"
     gene            complement(70657..72666)
                     /locus_tag="PC414_000255"
     CDS             complement(70657..72666)
                     /locus_tag="PC414_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2462671.1"
                     /translation="MKTIAVVTDGLSKLECFLNKNLEMIFKDKVMIKNYYLNKITEKE
                     LIKDDIILVMIDDRVLKIKKYVEDVSKIITINRSIKQKDIYKLFSLPEGIDVLVVNDN
                     KHTVLETISNLYNIGINHLNFIPYNPNEEYRNIKIAITPGEASLVPKYIEEVIDLGHR
                     YIDSSTFIQIIAKLKLDDKEITERLIKYTDEIISLYSGINTKYKELTIKADELNSIIN
                     LSNLGMAMVSDKGNIIICNNSLGDILDIQSNIIGKNIDELENENIKKIFSLSKAHDEV
                     IKFNNKYLNVNKYNIESFGRVTGYYFCIQEITYIRKLEQNLSKKLREKGQVARYTFDD
                     IKTVSTKMERTKKLGMKIAESDYTILITGESGTGKELMAQSIHNASPRHSQPFIAINC
                     AAMPENLLESELFGYEEGAFTGALKGGKKGLFEQANNGTIFLDEIGDMPIYLQSKLLR
                     VLQENQVMRVGGENVIDIDVRVIAATNKNLLEMIKGDRFRADLYYRINVLPINIPPLR
                     ERKEDIEVMLKHFMKRKRAISQDVQDIINAYEWPGNIRELKNTAMYINIMSSEDVILI
                     NDLPHNLLNITEDFSKEISILKDRTSIEKVKLVMECLKNANNLNKNIGRNGIVNSINE
                     LGYNITEGEVRGILNMLKELDLIICESGRKGSELNRRGKNILNSI"
     gene            complement(73104..74537)
                     /locus_tag="PC414_000256"
     CDS             complement(73104..74537)
                     /locus_tag="PC414_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDF2462672.1"
                     /translation="MALRKDFLWGGATAANQCEGAWNLDGKGPSCSDMCTGGSQKVSK
                     RITRVIEKGTFYPSHEAIDFYHRYKEDIALFAEMGFKVFRFSIAWTRIFPTGMEKEPN
                     EAGLKFYENVIDECLKYNIEPLITISHYEIPFAITEKYNGWVSRDVIDLYMNYCETIF
                     KRYKGKVKYWLTFNEINSATMPMGGYLSQGILNEGTTDFINQVDIPQLRFQGLHHQFV
                     ASAKAVKLAHEIDSNYQVGCMQIFATMYPYTCNPDDAVKTQRDSRVMNYFCGDVQVRG
                     EYPTYMNRYFGENNIEIKMEKGDLEILKEGCVDFYTFSYYMSTCVSSDSKEDNTSGNI
                     LGGVKNPYLKSSEWGWQIDPEGLRYALNEIYDRYRIPMMVVENGLGAYDKKESNGVIN
                     DDYRIEYLKAHIEQMKEAVEDGVDLFGYTPWGCIDLVSASTGEMAKRYGFIYVDKYDD
                     GSGDLSRIKKKSFDWYKQVIESNGERL"
     gene            complement(74624..76540)
                     /locus_tag="PC414_000257"
     CDS             complement(74624..76540)
                     /locus_tag="PC414_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2462673.1"
                     /translation="MRKYEQLSKEIIANVGGKDNISSLSHCITRLRFKLKDESIANDD
                     VLKKMDGIVTVMKSAGQYQVVIGNHVPDVYEVVCDLAGLGGDTIDNSDSDAPKGLLNK
                     FIDIISGVFQPILGVLCAAGMIKGFNAVFTALGLYGETDGAYIMLNAIGDAMFQYMPI
                     ILGYTAAKKFKLKPFIGMLIGAVLCYSGIQLDTLASTGQPLYNLFTGTIFESPVYITF
                     LGLPVIAMNYTSTVVPVILIVLLAAKLEKVFAKIIPDVVKNFFVPMAVLLVSLPLGFM
                     VIGPIATYASNIVGNGFLALFNFSPILCGALTGLLWQVLVIFGLHWGLVPIAMSNLMT
                     MGFDTILVGAFVPSFAQTAVVAAMYFKLKDKKIKELCIPAVISGICGVTEPAIYGITL
                     PRKTPFIYSCVGGGVAGAVMGIMNVKSYVMGGLGIFGIPNYINPTTGDMKGVYATIIA
                     IIVAIVVGFVLTLFFWKDDAIVEISKDDNGQGVIEKNKREVVYSPIKGEVKALEQIQD
                     AAFSTGILGKGVAITPKEGKVVAPFDGTVMTLFPTKHAIGIISDNGCELLIHIGLNTI
                     QLEGKYFKSFVNQGDKIKKGQILITFDIESISKEGYCLETPVVVTNYSDYVDILENTQ
                     KHVNNSQELITVLA"
     gene            complement(76614..77474)
                     /locus_tag="PC414_000258"
     CDS             complement(76614..77474)
                     /locus_tag="PC414_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2462674.1"
                     /translation="MIIEKILNNNVIITTDENHKEIVVMGRGLAYKKRTGEHISKDKI
                     DKIFKLSDPNISDKFKELIADIPIRYMELSDEIILYAKEKLGKRLNDSIYISLTDHMY
                     TAIERAREGVSVKNVLLWDIKRFYKSEFKIGLEALDYIEKKFEIRLSEDEAGFIALHI
                     VNAQMDQSIKTIYEITQIIQEISNMVKYHYRIVFDEDSVYYHRFITHLKYFAERIVSN
                     NLHENNEDDLLNVIKVKYKNAYKFIEKLDEFIHKKYNYDLTDEEKLYLTIHVERVVSK
                     SIKSDINPNN"
     gene            complement(77825..79012)
                     /locus_tag="PC414_000259"
     CDS             complement(77825..79012)
                     /locus_tag="PC414_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2462675.1"
                     /translation="MIESIKKRAYEIENELGKEIEEVCNFIFNNPELGEEEYISSKYL
                     VEKMKEYGFDTVYPYCNMETAFRAELGDNDGPTIAFLAEYDALPGYGENKEPAHACGH
                     NWIAASTLGACIVLSKLKENFKGKIVLIGTPAEETTGGKCDLVKAGAFDDVDVSYQMH
                     IEAFNNINCKALAIDSLEFSFEGVSAHAASHPHMGVNALDAVQLTFAGINALRQHVKS
                     DVRIHGIVSNGGEAPNIVPEKAACKFFVRAAERSYLDEVTKKVINCAKGAELMTGAKL
                     SYRYFENSFDNIINNKVLQKITKNNLIEAGITDILEGKDGPVGSTDIGNVSQVCPTMY
                     TEIALDISPMVYVHEKEFLNYANSEEAYDKLHKAVKAMVGCALEIYLEDGLLDEIKKN
                     HLN"
     gene            complement(79026..80417)
                     /locus_tag="PC414_000260"
     CDS             complement(79026..80417)
                     /locus_tag="PC414_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HDF2462676.1"
                     /translation="MSSLKKKKFTMPQTLTIIFLLIVLMAILTWIVPSGNFERVDIDG
                     RSVVVAGTYEKAPSNPQGITDVFTAPINGFIDAAEVVGFVLIVGGAFGIVNKTGAIEA
                     VIAHTVNKMKKFQFLIIPISMILFGLGGTTFGMSEETLPFYMIFIPLMTSMGYDSLTA
                     VATVFIGATAGFGAATTNPFSVGIAQALSQIVPGSGIEFRVVMFIIYMAISIGFVMMY
                     ANKVKKDPKKSLVHDISLNQELMVNSDTNIKEFTKREAMVIAIFTIGMAIMIYGVLRL
                     EWYITEIAMIFTAIGIISGIASGLKQDEIVNSFISGAGDLITAGLCIAFARGIVIIAE
                     NGFIIDTILNSAANLLNGLPKTIFINLTFLIEGLIAFLIPSASGLASLTIPVLAPLGD
                     LVDVSRQMIVTAYQFGIGVTNLITPTSGVLMGALAVANIPWSKWVRFVIPLMVVLTIL
                     VIAFLTIGLYLGF"
     gene            complement(81012..82712)
                     /locus_tag="PC414_000261"
     CDS             complement(81012..82712)
                     /locus_tag="PC414_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2462677.1"
                     /translation="MHYIAWHFNKNGGNNTMKDKLQVLSGAMMIPVILLVVAGVFIGL
                     GSAFANMENVSALGLSGLIKEGSFINVFFKVINDLGFMVMRYLPIFFVTGIAFGLSKK
                     EKGWGALGGIVLFIGMHTVISTLLAANGINSDTVTAEAFMASGLGEAEAYSRSSLYGS
                     FLGIFSYDCSIFGAIIAGFVASAVHNKFVDIELPSALSFFSGPRFSMIMMFIASIVLG
                     TAMYFIWPPVGLALSKLGNWIGSSGLLGTFTFGAMDKALLPFGIHHLIAFPIEYTRVG
                     GTMEVGGAIYEGVNNIRLAQMGDPDTLSYITRNFTTGRLLIHFAILPAAALAMYKLAD
                     NANKKKAISILIPAIVTAMLVGVTEPIEYTFLFVAPLLYFVAYVPLAGLTYVFTEMFN
                     VSIMGESFRNMFPNLLQPQKVDALPLLFLIPIFFIVTYIIFTWAIKKFDIKTPGRSSE
                     EVKLYSKKEYRERQSVDAGKTAYNNAVEEEVDDKDTKLVHSIIEGLGGSNNIKNVTNC
                     ATRLRVELNSIDGFYEDGFWVNELGASGVVKKKNSVQVIFGPRVITIASKLKAVLGVD
                     "
     gene            complement(82684..84087)
                     /locus_tag="PC414_000262"
     CDS             complement(82684..84087)
                     /locus_tag="PC414_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2462678.1"
                     /translation="MIVNKELIIDYLKSNQENIINDIRSLVEIPSIRDESTTDINQPF
                     GIEIRNAFDKLIQIAKDKDFVVKDFDGYAIHIEYGEGEEVVGVLNHIDVVPIYNKELW
                     KSKPFKVCQKDNYLYGRGVNDNKGPLIGILYALLFLRELNEKPKRKIRLIVGGAEETT
                     WECMEHYFSVNEQPKFAFSPDGNFPIVNGEKGILYFNLRKKIDKDKFRNHNLVDIKSN
                     KEDGFVCDKIEAVFKTNDKKDLVESLAYYTEIEELDEGKVLVRYTGERALSRNPHRSY
                     NCAFSLAKDLEKIKKLNDKGVIIRDILNSYFTDDNHGKKLGMYKEDVDMGVSTICIMS
                     IFLEKNELNMKIDFRYPKGISWEFITNRINEIGKKENLIVDIYKDLKLLYVKPDSELI
                     NKLSNAYKQGFGKEAELFTKGAASYARVLKNGVAFGPTIEGDNPNSHQANENISIDTL
                     YKAIEVYIYALYSLAFQ"
     gene            complement(84351..84842)
                     /locus_tag="PC414_000263"
     CDS             complement(84351..84842)
                     /locus_tag="PC414_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2462679.1"
                     /translation="MFNIFKKKENKIEKIYSPFKGNLVNLEDVPDAVFAQKMMGDGVA
                     IIPSEGKLYSPVDGEIVNVFDTKHAIMIKSNDNTNILIHVGLETMSLEGKPFDVKVKA
                     GDKVKAKDLIMEVDLDFITSNDLNIITPVVIMDDADSVKRTVKDRIEPGNVDINSIIM
                     EVE"
     gene            complement(84868..85680)
                     /locus_tag="PC414_000264"
     CDS             complement(84868..85680)
                     /locus_tag="PC414_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF2462680.1"
                     /translation="MRYKLICTDMDGTLMGKGFEVSEENIKALKEAMEKGIKVALVTG
                     RPYNAMKYFTSVLGDDIYIISTNGTYFKLLGYEYKKVLSKEALKKIYTIGEKYNLNKH
                     FKGCKIVISNNEIGEEHPYRLINSKNKEEDRIEIIENASCETLLEKADNEILKCILFS
                     ENVDSLREAKEEFKKQEDLEVVSSGKINFEVMSKGTSKGIAVKKFCDILGIDSKEVIC
                     IGDNENDISMIKFAGLGIAMGNATDEVKSMADFVTDTNVNDGVAKALRKILS"
     gene            complement(85693..86652)
                     /locus_tag="PC414_000265"
                     /pseudo
     CDS             complement(85693..86652)
                     /locus_tag="PC414_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422425.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
     gene            complement(86770..>87132)
                     /locus_tag="PC414_000266"
     CDS             complement(86770..>87132)
                     /locus_tag="PC414_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2462681.1"
                     /translation="QKLSTKLIKENDIICIEDLQVKNMIKNRKLSRSISDVSWSEFIR
                     QLEYKANWHGRQIVKVGKFFASSQICNKCGYKNEEVKDLNIREWICPSCNETHDRDIN
                     ASINILKEGLRLITIQNK"
BASE COUNT        28170 a        15679 c         9264 g        34019 t
ORIGIN      
        1 ttattttcat ttgctattta aaatcaattt taagttttct tcttataata aacttcctct
       61 ttgtggtctc atgttagtct ctaaatttaa aattttacaa aaaattttca taaaataaaa
      121 ttatattgat tgtatctaaa caaatagggc aataaaataa ttaagtatag ttacttacct
      181 ttcaagcaat attagtttta tgttagaatt ttattatatt gatttacagg aggtttgcat
      241 ttgaataata ttaattcact taaagtaata aaattatctc ttggatgttc tcttgctata
      301 tttattgctt ggctattaaa attagaatat tcaatggttg ctggagttat agtactactt
      361 acagtaaagg acactaaaag agaaactctt aaaggctcca ttggaaagat ttatggtttt
      421 cttttatgta ctattttttc atatttatgt tttaatattt taggatataa tttgacttca
      481 ttttcaatat ttatatttat aattatacca ctttgttttt tgttaaatat tcaagatgtt
      541 attgctatgt gtgtagtcat agcatcccat tactaccttc aaggagaaac ttctatcaaa
      601 tggattttaa atgaagctgg aatatttgca gtaggaactg gaataggtgt cttaataaat
      661 atgtatattc caacaaatat acataaaatc catgaagttc aaaaaaagtt acaggaagaa
      721 gtgagtattg tacttataga cattgcagat attattgtaa atcctaaaaa agaaaatgga
      781 tatagtagag atttatatac attaaattca cttattgaca gcagtatttc tgaaacttac
      841 gataatatta ataatacttt attaagtgat actagattct tcttagagca tatggatatt
      901 ataaaaagtc aacgggatat cttagaaaat ttgtatagtt atgtatctca actaaattct
      961 acaccaccac aagctcatat tctatctgct tttatacata agataggtta tactgaattt
     1021 gaagctgaaa caggcaatct tcttcttgaa gaattaaaga gactaatgct tagtatgaaa
     1081 aaccagcctc tacctgttga cagaacagag tttgaaaata gagctatttt attcctatgc
     1141 ttgactgagt taaaacagtt tttagttaat cgtaaacatg ctcagatgct tagagataat
     1201 aatttttata aataaaaagg ggagaaatct agcagttgac ctgactacaa aatatcagat
     1261 ataatattaa atgcgcccct agtaagaggg cgcaatgttt ataacttatt ctactgtcat
     1321 gattaaatca ttattcatat cgaacataca ttatttgacc aagtccaata ttagcttttt
     1381 catcagtgat ttctatttta atagtcacat catttttttt acagtacttc gctaattctg
     1441 caccagaacg atttttacca ctaaaatcta aagaaaatgg cttatcatat ttaaaaggat
     1501 attcaacagg tttaccctct ggtgctaatg caaacatccc atatggtgtg ttcataaatc
     1561 ctattacagc aatcgtaaga tttgatgggt ctaaatttga ttcactacct gtaaatgtaa
     1621 ttgtatttac tgatgaatta actacactac atcgaacacc ttcatacatt acatgttttt
     1681 catttatatc ttctcctttt ttcccaaaat ttccaacagc ctttgcccct tcttgatgtg
     1741 gtaacatagt gtgcttccca ggtggtgctg gtttctgtgt ctgttctggt tttaccgtct
     1801 gtactattgc accaactttt ggatatgctg tcttatcaaa tggtaatggg tggctaagac
     1861 tcttgtatat gatatccttt cctgctgatt tattagcatc aatatctaca tgatacaaac
     1921 gcccatcacc aaaacggata agattccact ggtggtcatt tgatgtgctg tgtacataat
     1981 aagattcaat tcccatagca tcacacaaag ctttcatgct cctagccatt cctgagcatg
     2041 cacctttttt agtaaccaat gtataggcag tatttgctcc ctctactgca ttccagtcaa
     2101 catatgttgt gttattttta atgtgattgt aggcaactaa aatcttagca tctgtggtca
     2161 tattctttgt gattttcttg ttcataaaat ctgctgttgc ttgtgctact gagtttagtt
     2221 gggcttgtga cagctttgga ccatatacgc tattggttcc agtgacatag ctttttggtg
     2281 tttttgctgt tacctctttg gctgtcatac cataaactgt catactcata cctgagatta
     2341 aaactattgt tgtaatcaat aacttggtag ctaacttcct aattttcttc atacttaata
     2401 ttctccttta taaaacattg ttacttccta ttctctacca ttaataaaat taatttagga
     2461 tttaaaaagg tggattgtca acatgtatat ttctccattc atacattcgt gaaatattta
     2521 aatttcatgg aaaatctaat agatattttc tagataattt gtatacaata caactcgcaa
     2581 atcagggtgt ttgctgttag tttgagaaaa tgatgatgat taatacattc ggaatctttg
     2641 acctataata aataccattg tcttcaatca ttaataatgg taagagtaaa gagaattatc
     2701 aataatacag ttaaaatcaa ctttgtatac atttcataca ttctctccca acaacattat
     2761 acttcttata atttgtatat tcaatacgtt ttctgggaaa atataggata tatctataaa
     2821 aatataaaaa aggagttgaa cttaaaattt gattaaaaaa aatcaatttt aagacaactc
     2881 atttgtatta taaaaatgct atatattatt attaagtaag tcaatatttt agatatttat
     2941 ctatttctta ttgacttacg cttatagtct aacttatttg tttagttgat atataaccta
     3001 gttttaagca ttattaatat ttaaataatc aagtatcctt tttgtttctt gtagtttctg
     3061 ttctgcctca tttaagtttt cttgaattat agaaatatat attatatcta tcaacgtaag
     3121 cattgctatt cttgaagaaa tagctccttc tctaagcctt ttttctatat ttggtactac
     3181 tagttttaaa tcactaatct tactaagatg attgtcacta tatttagtta tacaaatgac
     3241 cttacatcct tttaattttg cattttctat agactttatt acttcttttg attctccaga
     3301 gtaagatatt gctatagcta tatcttcttt atctacattt gaagatacca taagttgtgt
     3361 atgactatct aaagaggtaa atgcctgttt atttatcctt aaaagtttca tctgtaaatc
     3421 tagtgcaact aaagccgaac cacctactcc aaatatatat acatttttag cctttattat
     3481 ctgttcaact gcttctttta ttatatcttt ttcattcaac aaccttgttt ggttgatagt
     3541 ctctatgttt ttgtttgtaa ttttgtttat aacactatct atattacaat catagttttc
     3601 atctacatat gtgtattcat tttctttgcc catcaaatct ttagctatct caatttttaa
     3661 atccccaaaa cccgaatatc ccatttttct agaaaaccta actatacttg ctgtacttgt
     3721 ttttgagttt gacgccaatt ctgatacaga aagcttactt atagcctctg tattattaag
     3781 tatgtaatca gctatttttt tatctacttc cgttaaatct tcatatatct gttttaattt
     3841 taaaatgcaa ctcatactct atcacctcta cctatttact ataatatttc ttcttagcaa
     3901 gtaaacatgc tcctaaatct ggagtcaaca ctggtctcac tataccaaat ctcgttttat
     3961 tcaaatattt tttaagtggc tctaatatta aatctcctga cttaaacact ccacctgaat
     4021 aagatacctt tatctttgta ccttgttcaa aatgatgctc taatcctatt ataagcctac
     4081 ttaattcata agctgcatca tcaaatattt tactagcagt attatcacct tcaataactg
     4141 cttttaaaca tatcgtagca aactttgcta tttctattct atctcctttt atttcatcat
     4201 ttacatattt tattatgtca taatcattct ctatattaag atgtcttttt attaaatcat
     4261 ataattttgt ttttgaacac cttccatcag actgcattgt gaacattttt aaggtagcca
     4321 ttcctatata atacgctgaa ccttcatctc ctaatacata gccccatcct ccacatctgt
     4381 gaagattccc atctttatct aaagcctgtg ctattgaacc agtacctgca attatattta
     4441 tacctttttc cccatttaaa gaacctgcta atgctatttc tacatcatta cctagagtat
     4501 aatttatatg actatatgca ttttttgttg ctacttctaa tgcatacaat acttctttta
     4561 tttttccata tccagcaagt ccaacaaatg catacgttat ttcttctaca tttatcttag
     4621 catctttaca aacttcttcc aacccagtta ttaataattt cgttagatta tcaaatccta
     4681 tttgattata atgacaagtt cctttggtta tggttcctac tatatttaat tcttcatcta
     4741 ctaatgtaaa tgttgttttc gttcctccac catctacacc taaaaaatac atactatcct
     4801 cctacaatat ctttaataat tctttcaata cttaatatct aaaaataacc ttccaatgct
     4861 gtttttttaa tattgaaagg ttatcagtac tattatttat tttacaattt ttctattgat
     4921 tttaaagctt cacttatata accttcttgt ctatctagga tactctttgc actatcctta
     4981 tctaaaccac tttttatcat aaatatagcc aatttaacgc tattatctgc ttcttctaag
     5041 aatttttctg caacatcata ttttacattt gtagctatca ttattattct ttttgctctt
     5101 tcaaccaact tttcatttga tgtcttaaca tctaccatca agtttgagta tacttttccc
     5161 atcttaacca tagaagctgt tgttaacata ttacatacca ttttttgagc tgtaccactt
     5221 ttcattctag ttgaacccat tattacttct gcccctacta taatgtttat tggtaaatcc
     5281 gcttttttac tcatttcact attcggattc atagttattg atagtactgc acaccccaat
     5341 tccttagcat actccattgc acctataaca taaggagtcc ttcctgatgc agctatacca
     5401 catataacat ccttagatgt aactcctcta tctattatat cttgctttcc aagttcttta
     5461 tcatcttcag caccttctat tgctttaaat attgcttgat ttccaccagc tattatagct
     5521 tgaacaagct caaaatccac tccgtaagtt ggaggacatt ctgtggcatc tagtattcct
     5581 atcctcccac tggtacctgc tcctatatag aataatctac ctccctgttt cattctatct
     5641 actattatat taacagcttt tgctatactc tccttttgtt cttcaacagc ataggctaca
     5701 gttttatctt cgttatttat taattctatt atgcctaatg tatcttcttt atctatttgc
     5761 aatgtattct tatttctacc ttctgtaact aaattttcta aagtttttat taacatatca
     5821 tttcctcatt ttcttaatat taaattttta aacacatttt aattccaact ttcataatta
     5881 tatttttata ataaattaat attttcataa taaattattt tgttgagggt ttaaaattaa
     5941 acttttgcca tggtttaata taatctaata taaataattc ttcttttctt atatatccta
     6001 ctacattcga tttaccactg tttttcatat cagtcaaggc tatttgtaac tcccctgcat
     6061 aatgaccata ttcactactt tcaattacta tatctcctct ttttattatt ttaggagtat
     6121 taaatagttc aaatttatat cctttatatt taacacgaga ttgagtagac cttatcagac
     6181 tatcagatat gtcccctcta ttaaaatgta tttcttcaaa gagtatcttc ttctcaactt
     6241 ctcgtatatt ttcctctatt gctacatcaa atgtaaccat atcttttctc atagaaccta
     6301 atacctttag ctcctcatat gttggataac aatttgatat tattatatca tttatattat
     6361 tcatagctat tatatgcttt acttgaatat ctataggtag atttctgtgc atctcaattg
     6421 ttggaagccc atcagttaca ggccatggtc caaatgtagt ttcattttga cttgttacaa
     6481 aaactgctgt tcttaatcca tacttattaa atctttctgt acattttata aagtgttcta
     6541 aatttaatcc actatatcta tgtggataaa agttatgaca tcctatcaaa ttttctttat
     6601 ttggttggta gtccattatt gtatctatat aattagtatt attactcata ttgatttcta
     6661 ttttaagatt ttgaggatta aaagtcatta atgcctcttc agaacctgta aatcccatat
     6721 caagccttat tccgtctgcc ttcgtctttt taaaaaaatt caaatctttg tagcttattt
     6781 ctaattcacc aaatactctg ggatttacat caagtattac ctcaaaacct ttaaacttag
     6841 cgtattcatt tatactttta aattttttaa ttatattttc tttagtatct ttaactgata
     6901 ataaacatga aaatatacgt gtaaatccat tttcagcagc tttatctata taatcatata
     6961 tttccttaga tgttgttttt tctggataaa ttgatattcc taatcttccc atctgtgtca
     7021 tctccttata tcctatatgt gtttaaattt atttcgaatt acacgaaact tttttaaaaa
     7081 tttgatgaaa aaaagtttca tatatattta taaaaaaaga aaagtttacc ttttcttttt
     7141 ttataaacaa tcaacaaaat atcttcgtta cttatttcat aactgttata ctttgaaatt
     7201 tatactaatc taatatagtt tttaatttcc aatatagttt ctaattaata agcttcttgc
     7261 tcttcaactt tttcttcatg aagcaacctc ttatcgtaca tcttgacaaa tggatagtaa
     7321 attattattg atatagctat acataataag ttaagaactg aagctctcca atctccacca
     7381 gtggctaaat atgcccctac tggtcctggt aatgtccaag gagcagtaac tactactctg
     7441 gatactaaat tagcactcat agcaaaccat gaaactgttg ccattactat tggtgctaca
     7501 acaaatggta ttattaatat tgggtttaat actattggcg ctccaaatat tattggttca
     7561 tttatattga atattgctgg tattacagtt gcttttccta aagactttcc ataagctgat
     7621 tttgctctaa atgcaagaag taaagctaag cctattgtag ctccagaacc tccaatccat
     7681 ataaaccatt gaaaaaaggg ttcagcagca atacctggaa ctgcttgacc cgcagcatat
     7741 gaagctgtat tagcatctaa taattgtatc cataaaggtc ttgccaatga acctattatt
     7801 gatacaccat gtattccaaa tgaccaaaaa aacgttatta aaaatattag tataattact
     7861 gatggtaatg tgtcagtggc tgaaactaat ggagttacta atttagctat gaagccatgc
     7921 caatcaaacc ctaggaaata agtaacaata gccattaaaa ttattattaa tgcggctggt
     7981 gtcagagctt caaaagacct tgtgactgat gctggaactg cttctggcat ggttatcttg
     8041 aatttagatt tgtcagtaaa tctataaatc tcaacaccta ttatagatgt tacaatagca
     8101 acaaacattg aacctccacc aagattagcc attggcattt gccatccttg aggaattgaa
     8161 ttataccaag ctaaaagttc tggactcttt tcaactatct ttaataaatc aggggtaaga
     8221 ccctctattg cagtaggaac tattgtcaaa aggaatgtag ctgtagctaa taaacctcct
     8281 gtcaatccat ctaacttata tgactttgcc aaactataac ctatgccaaa tgtagaatat
     8341 actgatataa taaaaaatgt cattctatat ggtagtaata ttgtaactgc attttccttt
     8401 agatactgag ttacccccca tgaatctggt aatggaggaa atgctaatat caagaaaaat
     8461 gacccaacta ttataagtgg taatgaagct acgattccat ctcttacagc tcttaagtga
     8521 cgttgttctg ccaatctggc catagaagtt gataatttag tgtctaaaaa ttctgtaaat
     8581 ttatctagca tcttttctct ccttaattaa tatttatttt tattatcatt aaaattttta
     8641 taaaattaac tttcttctaa tctaacaaca ctgcaactac tatttactca acagctcttg
     8701 aacttgtttt aataactttg gtcctcctaa tggactataa gcttgtgctt gtattaatgc
     8761 acatggtaca ctaacctcat cagcaaaagc ttttaaatag tcaaatctat gtctaacttg
     8821 tggagctacc atagcaatat cccatccatt tttaacttca tcctcaaact cttgagtacc
     8881 aacagccaaa acatctatat ctattccatt ttttgctcct tctttttgta atgcattaac
     8941 tgctatagca cttgacatcc ctgatgaaca aacaaataat actctcatat tgattccccc
     9001 tgttttttta tttcttttca atcattatag catgtatttt tgattacagg aatacgtttg
     9061 tatatatttt tttcatattt tacattttaa attgtaattt tatttcatac atttatatct
     9121 tgctttaatt gctttattta tctaatcatt cttttgtcta aaattacaca ctacttaaaa
     9181 tactcatatc ttttcttaaa aaagaaaaaa gactatctta ttattaacat aaaatagtct
     9241 caatactttt ttaaatatgt attcctttat atatccagaa gtaacctatt attgcaaatg
     9301 ataaagcaaa taaaatatac agtctaaaaa atcccaaaga aaaacctctc ttagtttcaa
     9361 caaagtatct tgtatatgca actgcaaaag atgtaattaa tgaatttcct ataacaactc
     9421 caaatctatt atctattcca aatatagata ataacaaagg aaaaactaca gtacttacta
     9481 gagatgaaac tattaatatt aatccacttt taaaacttat agttggaata ttataatctg
     9541 ttctttgatt tttctccatt tgtttcttag tatttttatt actttttgta ctttttgcat
     9601 ttttataatt attatttttc tttcttttct tttttttatt attagacatg tcatttacct
     9661 ccaaaaggtt taggtgttat attccctaaa ataactttat ttttagcccc tgttgcagat
     9721 attacattag aaaaagaatt atttaatgtt tcattgccca agattacaaa tgccaaagcc
     9781 tcctttgcat cagaagaata cccatattca tcttgagtta atacttcaac ctctaataat
     9841 aactctttta tatatcctat taaagttaga ttatgagctc ctccacctcc aattaataaa
     9901 gtatctacat taacctttgg aagtatgaag tttttataat tataatatat agcttttgcc
     9961 gtaaacatag ttactgttgc tatcaaatca tatttatcta catgtttata tttgttaagc
    10021 atgttatcca aataaacttg tccaaatacc tctcttccag tgcttttagg tggcgcttga
    10081 ctaagatact tatgactcat caaatctttt aacatttctt cattaatttt accttttgat
    10141 gcaaagtatc catttttatc atactttcta ttaaataatc tttgacatac tccatctatt
    10201 atcatatttc ctggtcctgt atcaaatgca aatacatcat ctatgttaca tgatttaggt
    10261 attatagtaa tatttcctat tccaccaata ttttgaagtg ctaaattttt attgttatca
    10321 ctatataata aaaattcact atatggtact aatggagcac cttctccacc tgctgctata
    10381 tccattactc taaaattaga tattaccttt gtatttgttt catatgctat aactgctggt
    10441 tcacctattt gaagcgttga agatacaaaa ttattataac ttcttggtat atgaaagatt
    10501 gtttgaccat gagatgcgat aaaatccaaa tttgctatat gaaagtttgc tttatgacac
    10561 actgatttta cagctttact aaataaatat ccaagcttaa aatttaagct acagattaaa
    10621 tcaacactcg attcctcttc tatacagcac tttcttattt cgtcttttat atctttagga
    10681 atttctagag ttataaattc tatttgctta actttagtat ttctaccatt cccactaatt
    10741 tcagctagta ctgcatcaac tccatctaag gaagttcctg acattaaacc tacactatac
    10801 atagttatca tccttttaag tttatagtta ttcctcccca aatcaaggta ttaagtttaa
    10861 taattttaga attaatctta tttcatcttt aattacgata atttactctt atgaaattaa
    10921 ttgagttaat cttatacacg ttttttacct tagctaaatc ttacattaat ttattttcag
    10981 tgtcaatgta ataccatgta attttttttc acaaacaaaa catattctct ctccaaaaac
    11041 catattaaaa tagttactac aaataaaaaa cataccaggt acctatctga tatgtttttc
    11101 atatataata attattattt tataatatag atagagcatc atttaaggca ctaattaaat
    11161 cttcactatt ttcaagaact aaatttattc ttattgtata tgtcgatttg tatatgatga
    11221 tagattttat agtctaataa cctaactatc taggcttaaa taatccgata tatcataaaa
    11281 atagatatta aaaattgtat atgaaccatt ttccgaacat acctcaatct catgccctaa
    11341 actatctatc attttcttta caaaatacat ccccattcca gtagacttat acaattgatt
    11401 tcttccattt ttccccgtaa atcccttgtc aaaaacccta gatatatctt cctcatttat
    11461 acctatacca aaatctttta tagataatac cactccattt tctaactttt ttgaattaaa
    11521 ttcaatctta ccaacttcct ttgaatactt tatagcatta ttaataattt ggtctaaaac
    11581 atatgacatg catttagaat ctgtgtaaac atcattttct aattcattca aactaacttc
    11641 tacattattt tttataagca aaaatgaatt attttttata gacattttta tcaatttttc
    11701 taaattaaat ttatttataa atacatcttc aaacatagtg ttgcttctac tactatacaa
    11761 aatagagttt actaaaaaat ttatctttgc aacctgattc ttaatatcca aactatcttc
    11821 tatgctactt aacctatctg taattatact taaagaagat attggaattt ttatttcatg
    11881 aacccattta gctatgtact catccaattc ttttaatgat tgtttataag tctctatgtc
    11941 actattattt tttattttta agttttctat aatatctttt attagatttt cttcaaaaga
    12001 ttgtccatca actaaataac tgtctatttc accactattt tctatacaat tatataaatt
    12061 tgtgtaacta tttctaaaat ttatataatc tataacaaaa aataccatta ttataaacag
    12121 tactaaaaaa tctaaatata ttaactctga atatttattt ttaaaaatat ctatagcaaa
    12181 taagtagata tttattatac taactacaac taaaggtaga gcaatatact tagacctacc
    12241 tttcaaatat tttactagca ttgtattata tatcctaccc ccttcttagt ttttataaaa
    12301 tcatcaaaac ctagttcttt aactttactt ctcaatctat tcatatttac agtaagtgca
    12361 ttgtctgtca caaactcatc attatcccat agagtcatca taagactttc ccttgataca
    12421 accctattct tattttttaa taataatgtt aaaattttta tttcgttttt agtcaactct
    12481 atacttttat ccttatactt atgttttata atccctttcc ccacatcaaa catcaaatct
    12541 tcacaataca aaatactatt agaatcacta taattataga ctcttcttat tataccattt
    12601 attttggtaa ccaatatttc tatagaaaat ggtttggtaa tgtaatcatc tgctccatta
    12661 tttataccca ttattaaatc catatcagaa tctcttgatg ataaaaatat taatggtaca
    12721 tttgaaatag ctcttatttt ttcgcaccag taaaatccat tatataatgg taaatttaca
    12781 tctaaaagta ctacatctgg ttttttatct ataaattcat ctaatactac ctcaaaatca
    12841 tcaattaatt ctactttaaa tccccatttt tcaagtgcta atgcaatctc ttttgataaa
    12901 tcaatatcgt cctctactaa taaaattcta tacatttgta tattctccat tatcctaata
    12961 tttcactatg ctatgaatta ttaatatcat aacatatttt atatgaattt attacccaaa
    13021 ttatttagtt aatcaacaag gagactctta ttacaagaat ctcctttctc tttattaata
    13081 tatcaactaa tttttataca ctacactctt aaacttatta acagatactg ctccatatat
    13141 tatgaatatt atcaaacata tcactaaact tagtatatat ataccaaata aatcggtcat
    13201 caataaatct cctgctaatc tcaatgcaaa ataactagat atagttgcac ttaacattgg
    13261 caaagtataa aacatagcta cttctttaag tatagctcta tttatatctt tttctgtagt
    13321 acctattttt aacaaaatct tatatttctc tttatcttca tatgcatcac tatatatttt
    13381 catatatatt atacttccag ctacaattgc aaagactaag aataaaaaca caagcactac
    13441 atatgcaaac ttcatccatt gactctcacc ttgagcttta tatccatttt gaatataccc
    13501 attttttaaa cttaaattct tttcaatatt gttgaacatt acttgactat tttcttcttg
    13561 aaataccttt gcaccataaa aatacacttg ttgaccctta tctcttagtt taaaatatgt
    13621 atcatcattt accactataa tactttgatt aagtcctgtc cctagtactg gaacttttac
    13681 ttctcccctt gaaatattat ataactgacc atcaagtttt atcttggctt cttttgtagc
    13741 tattgatgct atcgtttgag ggctttttaa ctctattaca ttatttccac taactaatct
    13801 ttcaccatac tcttttaatt ctttatcatt gccattcact ttaagagtat tacaaaaatc
    13861 actatatttc attatagtat ctgttgtatc ataagcttct tttatatctg ttttctttaa
    13921 aattgttagt tctggattta ctgagtactt tatcttatta gtatctttta ttatattctc
    13981 tatttgtttt ttattcactt catctttatc cattgaaact atgctaatag tgtacacatt
    14041 tctttgttct tgggcattct tatgtatacg cttcatcgca actgaagcac caagagttga
    14101 tattgttgta gttataatta tagctattat agcataagtt ttatagtttt tatttaatcg
    14161 gtagtttata ttgtttattg caattatatt atttccatta aataatattt ttttattttt
    14221 gattataata tcaaatataa caggcataac tgatttgaat aatccataag tacctactat
    14281 tattatacct ataacaggta gtacactagt tatatctcca gttcttatat tggtagaaag
    14341 atagtagcca tatcctatta ctataattga tactatagct aatatatata tacctattcc
    14401 tacctttggt attttttctt gttttttaga tatattgatt agatttatta cactacttct
    14461 acatattgtt ataaaacctt ttacagccat tattataaat atagaaataa agattaaagt
    14521 agcatatagt atagcttcta tactaagtcc attagatacc tttatattaa atccagataa
    14581 ctttattatc actacttgaa aaaatctaga tgttattatc cctataatta ttcctgttat
    14641 acaagaactt attcctataa gcatattttc tacaaaatat atttttccta tagtatataa
    14701 atctaatccc ataaaagaca atattccaat ttctttagac ctattcttaa aaaatatatt
    14761 tgttgcatac catataaaga aaaataaaaa tactgatagt atcattatta cagctactaa
    14821 cactgatagt gtaaattttt tattttcatc ttgtaacaca tttatagcat caccatatct
    14881 taataattca aaattagtta gcacaactac tgaaaaaacc atagatataa aaaatgctag
    14941 ataagttctt atgttactct taaaattatt ataagcaatt ccaagtatac tcacttacat
    15001 ctcacctccc atagaggcta gcatatccat aatttttcca taaaattctt ttctactatt
    15061 tcctttgttt aatttacact ttatatttcc atcatttatc atatatactt catttgaata
    15121 acttgctgtt agagggtcat gtgtaatcat tataatagta gcgttatttt ctctgtttat
    15181 tttttctaat gattctaaaa gttcatatgc tgatttagag tctaaagcac cagttggctc
    15241 atcagcaaat atgactgttg gatttgtaat taatgaccta gctgctgcgc ctctttgttt
    15301 ttgtcctcct gataactgat atggatattt atctaaatga ttttctaatc caaatttttt
    15361 agctatagaa aataccttag cttctatctc cttacttttc ttttttccta atgctagagg
    15421 aagtgctata ttgtccatta gtttcatatt gtctaataga ttatagtctt gaaatataaa
    15481 ccctattttt tctcttctta attttgatag ttcattattt tttacttttg atatattaat
    15541 tccatctagt attatttctc ctgaagtttg tttgtcaagt gtagacatta aatttagcaa
    15601 tgttgtcttt cctgcaccag atggacccat tattgatata aaatcacctt cattgactgt
    15661 aagagatata ttttttaaca catttgtttt aaatcctttt attccataat cttttgatat
    15721 attttttatt tttaatattt ctttcataat aatagcctcc agttgctaat tttttaatat
    15781 atatttattt taaaggtttt atataaataa atccttaagt aaatattaca ttttaactgt
    15841 tagcattaca cttttgtaac atatggaaga ctattataat tatagtagaa atatcttaaa
    15901 ataaaagcat gatttacttc tttgtataaa caaagaaaag aagctcctaa gaagcctcta
    15961 atctatacaa ttattcttct ctataaaaaa ccaaaataca cattgattgt ggcaaataca
    16021 gatactataa acaagtgata ttactactat atattaaaac aattattcct gaagataatt
    16081 caataagact atcaagcaat aagctaagca tccaaaaaga cttaatacca ttaatgtata
    16141 attataccca atattaatgc tcaaaaaacc agcaacagtt ggaataatca gagatgaacc
    16201 tcccataaat atacttaaag ctattaattg gctactactt ctaagttttt taggagaaac
    16261 ttcaaatatt aatctttttg aagtaattaa aattaaagga gtagaaaaca cttgaaaaac
    16321 acacagtata ataatgatat ttgaatcatt tgttaaagca aataatggaa actgaactgt
    16381 tatcataata gcacttaatt ttaataagtt taaactgcta tattttttta ataaataaga
    16441 accagccaga tatgttggta tctctaacaa tgactgtaaa gaccacttta aagatatctg
    16501 agaatttgta gcatttaaag ctatcatttt atctattaca gtacagttat ttgctaccac
    16561 cattgagtat aataagaata aaatacatac tagtaagata tattttttat cttttaataa
    16621 ttcaataata tctttattag ttacatttat tttcttatta atcttttcaa tatcaggtaa
    16681 taatagtata tttataataa gtaatactac aattaaacca atcgctacag ataatccaaa
    16741 atatgagaat ctaagaatca agtaagatgc taccatactt ccaatagccc atccaatcga
    16801 cccaaaagtc ttaataaatg acaaataatt attaatatta tctccacaac ctattatcca
    16861 tgtatcatat aaactacagc aagtatttaa taatcctcct gataaagcaa caagtaataa
    16921 gtatattata aaagttgcag attcagtaga aaacataata actgatgcaa ttccataaac
    16981 tgataaacta attaaaacaa tttttttcat tgtttgatac ttatcagcta atactccaaa
    17041 taataattga aacaatatat tggttattgc atagctcgat aatataattc ctctttcaaa
    17101 cgctgagtaa cctaattttg ataagaaagg tgtgaatttt gttgaagcaa aggcaaaaat
    17161 aatatatgag aaaaaataga atatgataaa tttaaattta atcgctttat taatcataat
    17221 caatcaaaat tgataacttt aataccacta tttccttcct gatttaagtt tgaaagatga
    17281 tattttaccg tattaggatt ctctacaata actaaaggaa tatataaagt attttcaagt
    17341 aacttattat caatttctac taacttttcc cccatattaa cttcttgacc ttgtttaact
    17401 tctaatttaa agttttcttt ttttgttgtt gcactatcca atccaatatg aataagaatt
    17461 tgaacatcat tttgcaattt aattcctata gcatggttag tttgagcaat aaaacttatt
    17521 actcctttag tcggtgattt aagaatattg tcctttgcta tcatagcaaa tccatctccc
    17581 attaccttag ttgaaaatac tttatcagga atttctccaa gattaattat ttttccatca
    17641 atgtatgaat taattgtaat tgcttctttt tttcttttaa agaacattat agacactcct
    17701 ctacataatt aactttattc taattatcat tacaaataac gatattaagc atactgcaat
    17761 tgaggaaaaa agagccttat agctctttcc ccattgtagt tctttaatac tgcttatgat
    17821 ttgtttaatt ccaacaaatt tataaatata ggtaaacatg ataacaatag ttattgtgta
    17881 aatttggaat atctgcccta ataagactaa atagaaagca atcatgtttt cttttatctc
    17941 tttattcttt tttatattgt aaataacata tgcactgtat actgcactaa agttagagat
    18001 taaatttaaa cttgcattaa catcattgtt taaaagttgt gaaaatgaaa tatttgtaaa
    18061 taatgatgtc aattctaaat acactatata taaaacaggt aatattagaa aacaaaccat
    18121 aaccactgaa agaaatatat tttcatcttt taatactgct gtgatatttt ttattttatt
    18181 tgttatcatg tttttgttct ccttatttaa aagttggcca gaaatcctta ttagccacaa
    18241 tcatttcatc taagatttct tttgctactt ttgcacttgg aacagtttta ctcatagtaa
    18301 acgactgcca taatttttga taactttttt ctatccatgc ttctacagct aatttttcag
    18361 aagcaacttg ttgttccatt aaaccttttt ggaaagttgg aattttacct atagacatag
    18421 gttcaaccat atcttttcca acaaggcaag gaatttcaac cattgcatca tctgcaaaat
    18481 tttcaatagc tccattatta ggaacaatca gtaaaaatct ttcctgagta ttaaatgcta
    18541 atgcacaagc taattcaaca ataaattgag aatgttcact tgcatcaaac catgtatctc
    18601 ttgctgtgcc attttttata attctttcac attcttcgaa tgtatccttt tctcttctat
    18661 ccataatttc atttgctctt gtataatttg gattagcatg ttcaacagtt tcatcagcca
    18721 ttaaataata ttttaaataa gtattaggta atgtagtcgg ttctatttcc aataaatcct
    18781 ttgctgctct catagtttct aaccaactag attctttgtg ttgaccttga ggtgtaaatt
    18841 cctcatatcc ttttttagca caatattcct gtaatttagg cattaaatca tttccttctt
    18901 tgtccttaat acttgtccac cacccgaaat gattcaatcc ataatatcta acatcaaagt
    18961 ctttacgaga atttaaacct aaaactctag caaatatttc ctctaaacat actggcatat
    19021 cacaaatatt aataattctt gaatttggtc ttaatacacg acatgcttct gcaactatag
    19081 ctgctggatt tgaataatta agcatccaag catttggaga atatttttcc atataatcaa
    19141 tcatttcgat tattggacct attgttctta atccataggc aattccacct gccccacaag
    19201 tttcttgacc aacaacccca tattttagtg gaattttttc atcttgttca cgtaaagcat
    19261 ataaaccaac acgaatttgt gccattacaa aatcaatatc agtaaatgct tcttctggat
    19321 ttgttgtata actaaattta atttctggat ttttctctct aacaattatt gcacatgctt
    19381 cagcaacttt tctttgtctc tcatcattat tatcatataa tttaattgat tcaataggaa
    19441 atttagcaac actttcaatt aacattgtta caattccagc agtataagta ctacccccac
    19501 cagcaattag tatcctctgt ctttttcttt ccatctttcc ttctccttta taataaattt
    19561 tcaaattctt ctcttacact tggtactgaa agcccaataa taatctgaat agcttttcca
    19621 ttatgcacta aaccatgtgc acctactgcc ttaaaatctt cttctttaga aataattgag
    19681 tcatctttca aggttaatct caacctcgta gcacagtttg taacatctac aacattttct
    19741 gctccaccaa ctaattttat gtattcagat gcttgttgag aggctttact caatttattt
    19801 cctttttcat ctttagtttc taagtattca tttttagtat atagttttgt ttcttcacta
    19861 tcttcctctc taccaggagt tttcatatca aacttcttaa ttaagaaagt aaatacaaag
    19921 aaccagataa atgaaaatat aatccctaca acaacttgaa caacatatac cttccagtga
    19981 tacattccaa gaggaagcca atttaataca acccagttaa taattcctcc accaaaatca
    20041 ccaacaactc ctagggcaaa cgaaatcgtt tgcaagcatg catctaaaaa tgcatgtact
    20101 aggaataaaa gtggtgctac aaataaaaat gtaaattcta acggctcagt aattccagtt
    20161 aatactgcag taagagtagc aggaatcatt aaacctaata cttttttctt tttttctggc
    20221 tttgcagtta catagaaagc accaaataca cctaatgaac caaacacctt cgataatccg
    20281 gataatgaaa atccgattgg atacatttcc tttagagtct tagctgaagt ttgaaaatcc
    20341 tgaatatgag tagcccaata tactgatgta ccacctggaa caacaacact atcataacaa
    20401 attggtgcat atacaaaatg atgtaatcca gttggtatta aaatcttttg taagaatgca
    20461 taacaccaaa ctcctatatt tccactattt ataaaaaacg tttgcaaatg tctaataaca
    20521 ttttgaatat gaggccaaat aaaacaagtt aataatgcaa ctggtatcat aacaaagaaa
    20581 caaattgcaa ctacaagtga tgagcctcta aataatccaa taaaatctgg cagttcttta
    20641 tcataaaatc tattatgaat ataaatgaca atacctgata ttaacaaagc accaaccata
    20701 ccagtatcta atgttttaat accagcaatg cttacaagtc cacttccagc acttatttca
    20761 gctgcgaaat caactccaaa gctttttccc caaaaactta acatagaacc tacaaaataa
    20821 ttaaaagtca aatacgttgc taaagcctcc atacaagctc ttgcttgttg cttttttgca
    20881 agtgctattg gtaaagaaat agcaaataat aatggcaatt gattaaatac catccaagct
    20941 ccactagata ttacacccca aaattggtac caaatatttt ctggtgtagc aagtgaaccc
    21001 ataattgact gattttgaaa tacaattgag aatccaacca ttatcccagc aaaagcaaat
    21061 aatagtgttg gtgtaaacat tgcccctcca aaacgctgta ttttcttcat catataaatt
    21121 actcccctcc tcatattttt agtttatctt tggccttaga tatctattat tttaacaggt
    21181 taataaatat atacaatctt tagggtacat tcggagctta ttcacagtca aataagtttt
    21241 tgcacatttt tatgttcaaa ttctcataaa aaaaaagaat gactacaatt tgcaatcaat
    21301 cccttctgta atcattctat gccttttata aatactatat ttaataaata ataactcaat
    21361 caatataaaa tataacattg tcaatttata actagggtgc atttgtaaaa atgagatatt
    21421 agtcgctgaa atataaattg attcatcact taaatcagtc aatgtattat ttttaaactc
    21481 tgtaattgaa attattttag ttcctattaa ttttaatctt ctagcaatat taagtgtatt
    21541 ctcattctca ccacttaggg atataataat cataacatca tttttactga caatattgta
    21601 aaaagaatca tcaaatgttc ttccctcaaa atcatatatt atttcttgac atgataagaa
    21661 cattctcttt atctgttttg caaccacatt ttgaatatca cctgaaccat aagcgtatat
    21721 tgttcttgat tcataaagaa gtttacacaa tgcatcataa tctttatttt tttgatcttc
    21781 aacatactta attattgaat tacatgcaat atcaaatgac tgattttcta tgacatgaac
    21841 cttttctgat tcccagctta ataatacttt aaactcactg aaacctttta aatctaattt
    21901 tttaacaaat cttgtaatag ttgcacgaga cacattacat ttcatagcaa gttcatttat
    21961 tgacatctta ctacaatgtt ttttatttgc agttatatat ttccatatta ataaatctgt
    22021 atcattcaat ttattataat ttaaaaaaat catttcttct aatctcataa actattaccc
    22081 cctttaatta aaaatcgtca taaatattat accaatattc aacattaaat tacagcgtat
    22141 tcttatattt actatttagc ccataaaatt gactataaaa aatactgcta taattgatgt
    22201 ttaaaaaaat gtagttttaa ttttattctt ttatctaata attaatattt tcaagttcta
    22261 gtgcttttct tatacaatta tcatctttaa aatcagcagt ccttttacaa tcttttactt
    22321 taaattctgg aatagtttta aattcctcat cacatattcc attcttattt aaatacatac
    22381 aacttgccat tctaactatt agaccactat tttttaaatt aaacaataca gggtctatac
    22441 accctccatc tccgccagta gtttcaccaa ctaaagtcgc aaatttattt tctttacaaa
    22501 acattgcaaa tgactctgat gatgaatata tttttctatc aacaagtaag tatatattac
    22561 ccttaaattt tgaatcatct gtagatttta tagtatagga agtatcttca aaatcattaa
    22621 aatcttttat aatttcttta ggtgcatttt cctttacact tgctggtaaa ttttctacag
    22681 gatttaattt tatatttctt gcatttgtat atttttttac tatttcatta ttatttctat
    22741 aaattctata accatttatt ttgacatcat tttttatgag tttagatacg attccctgcc
    22801 aatacctatc acttccacct aaattacctc ttatatcaat aattaaagct ttatgttttt
    22861 ctaaagtgtt aatataatct ccaattattt ttaaatcatt atttatagag ccattctttg
    22921 atgacattga tggtaaatac atatatccaa tctgaccctt aataacatcc tttaaaatta
    22981 attctttttt tacaaaaata tcatctggca tttttatttt aagattcata gagtcatatc
    23041 tatccacaac ttttttatca ttaagaaaat cataccaatc attattagag tatgatttct
    23101 taaaaagttc atatcttttc ttattatcta ttacttctgt gtgtttatta tttaaatcag
    23161 ataatataga tgacagttct tctataaatt cttcatcatt ttcagtgttt tttattcgtt
    23221 ttgtatattc atccttattt gctaaccaat caatattatt aagcctctta tttacaccta
    23281 gatatggata tcctgtcttt acaacattat acacataatt aaagtcatcc accttttcct
    23341 cttcagacaa ctgattcttt tgagaataag tagaattacc taataatatt ataaatgcta
    23401 aaataattac taaaaataat actttctttt tcatgtaatt ctcatccact ttcagatttt
    23461 atattttaat acattgtatc attattttat tatcacctaa acagttaaaa gaaattcttg
    23521 aagatatatt ccactattat tctctttaaa aatttttatt tgtattttca aaattatttc
    23581 atgaaagtta taaaataaca attatattca aatatctatt ttactgctat atcctgaaag
    23641 taaattctta tcagtgatat actaaaaaag atatgctaaa aaatttatcc attataatga
    23701 cttaaattta gatactttac tgatttttat ttattagttg agaaagatat tgataattaa
    23761 aaatattgat aactatagaa atgattattt tttatagaaa ttttaaggat ataaactaat
    23821 tatacttaag tctatatcct taatttatca tgattatgag aatttttaca aatacttatc
    23881 tttgtccgtt ttagaataaa aacaagaaca tatcctgtta ttttattcaa acatccaagt
    23941 ttaattacta cacaattact tattagaaat tgtatctact tcatatatat atctgttaag
    24001 tatagcttct agcatttctt gtcttccaga cttattcttt ataactggca tttgcagtgc
    24061 ataagattct aactctttaa atccaacctt accttctact atatccttac ctataccttc
    24121 actaaaactt gcatatcttt ctttagtaaa gttttcaaat acctcatctt ctaataattt
    24181 atgagctatt aataatccct ttgcaaatgt atccattcct gcaatataag ctaggaataa
    24241 gtcttctacc tcaaaagatg ctcttcttac tttagagtca aaattaaacc ctcctggtgc
    24301 tattccacct tgctttaaca cttcatacat agcaagtgtt gcatcatata tatttgttgg
    24361 aaattggtct gtatcccaac ctaataatag gtctccttga tttgcatcta tactacctaa
    24421 aacattgttt attcttgcta tatttaattc atgttggaaa gtatgtcctg caagtgttgc
    24481 atggtttgct tctatattca ttttgaagta tttatccaga ttatactttc ttaaaaatcc
    24541 taaaacagta gcagtatcaa aatcgtattg atgtttagta ggctcttttg gcttaggctc
    24601 tattaagaat tgtccagtga atcctatttc cttagcatag tctacagcca tttgtaagaa
    24661 tcttgcaaag ttatccattt caagttctgt attagtgtta agtaaagttt cataaccttc
    24721 tcttccaccc cagaatacaa aattttctcc tcctaagtac ttagttattt ccatagcttt
    24781 tttaacctga gctgctgcat atgcatatac atctgcatta catgttgtag atgcaccatg
    24841 aacaaatctt ggatttccaa acatattggc agttccccat aataacttta tacctgtctt
    24901 ttccatatta acttttatat actcaactat ttcatctaat atcttatttg tctcttctaa
    24961 atctcttcct tctggtgcta tatctctgtc atgaaaacag aaatactcaa ttcccatctt
    25021 agacataaat tcaaatcctg catcaacttt aatctttgct atctcaattg ggtctgttat
    25081 attattccaa ggtctctcta ctgtgcctac tccaaatggg tcattaccct ctccacataa
    25141 agtatgccaa tagctcattg caaatcttaa gtgttctttc atagtcttat tacctaccac
    25201 ttgttcagga ttgtagtatc taaatgctaa ttcattgtca gattttactc cttcaaattt
    25261 tatctgtcct attcctttaa atatttcact catttcatgt cctcctaata ttttacacat
    25321 ataatatatg tagtatgttt tcttaattta aaactatttt ctatctttat ttttcttttt
    25381 aagctaattt aattatttat ttttgcatta actacttcat ctaataactt aaagtcatct
    25441 ttcaaagatt tgtatattct tttatacaat ctatgatata tattgtatgt atctacattt
    25501 tcacttattg gttctaattt agttgaagtt tttattatag catcacaagc tttttgtata
    25561 ttttcatata ttccagctcc tactaatgca agaattgcaa ctccaagtgc tggtccttca
    25621 cttgaattta tagtatatac atcttgatta aacatatctg cttgcatctg tctccatact
    25681 ttactttttc cacctccacc tgaggctctt acttcattaa cgtctatatt aagattctct
    25741 attatatcca tacagtcttt aagactatat gaaacacctt ccattattga ccttgcaatt
    25801 tcttttttag aatggattgg agataatcca aagaacacac ctcttgcata agggtcaaga
    25861 tgaggagttc tttctcccat catatatggt aagtaaagca atccattaca tcctgttggt
    25921 actttactta cttgattatt tataatctca tatacatctt cacctaaaga atttgaaact
    25981 tccacttcac tttgacagaa attatcttta aaccatttta atgacaagcc agctccttga
    26041 gtaactccca taacatgcca tgtatttggt attgcatgac agaaagtatg aactctacct
    26101 tctttatcaa tttttggctc ttttgtataa gcaaatacta cacctgatgt acctatagta
    26161 gatgaaacta ctccatcttt aactattcca tttcctatag ctcctgctgc ttggtctcca
    26221 gctcctccta ccactattgt tccttcttta agccctgtca aacttgatac atctctagtc
    26281 acataacctg taatctcttg tgattcataa actttaccta aaagattttt atctatattt
    26341 aacttttcta gtacttcatc gctccaatct ctttttctta tatcgagcaa ctgcatacca
    26401 cttgcatctg atacctctgt agcatattca cctgtcaatt tgtatcttat ataatctttt
    26461 ggaagaagta ttttattagt atttttatat atatctggtt cattgtttct aacccataat
    26521 atctttgatg ctgtaaatcc tgtaagtgct ggattagcag ttatttcaat cagtctttct
    26581 tcacctacta aatcagttat ctcctcacac tcttttactg ttcgttggtc acaccatatt
    26641 atagagtttc tcaatgtttt accttcattg tccagcataa ctagaccatg catctgccct
    26701 gataatccaa ttccttttat acattcactg cttatatttg aactttgaac tacttctctt
    26761 atagattcga ctacagcttt ccaccaatca tttgcatcct gctctgccca acctgatttc
    26821 tcttgtataa gaggatattc atatgtacaa ctttttatag tatttcctag tttatcaaat
    26881 aaaactgttt tcgtacctga tgtaccaata tccacaccta atacatactc caacttcatc
    26941 cctccttgaa tgccacttca taactatcgt tttcctatcc taattttttt atattttagg
    27001 tatattttac ttgctttatt tttgttagta tattaaacta acttatgaac taataataaa
    27061 atatttaatt atctttgtca atgcaaaata accttttatt tttgtgtaaa aagtattaca
    27121 atattatttt aaggaggaat atcaaattgg ttactgataa atacactatt cgagaaatga
    27181 atgaaagatt ggtacttgaa caaattataa aaaatggtcc tatttctagg gcaagtatag
    27241 cttctactat aggattgaat aaagcaacta tatccgctat taccaaaaaa cttattgatg
    27301 aatctctagt tcatgaaata ggaattggaa atagtactca tagtggcgga agaaaaccca
    27361 tattattagt atttaataaa tgtgcaggga tttctttaag tatggacatt ggatacgact
    27421 atatattttc ttctctatct tacttagatg gaactattat aaattctaag aaattaacag
    27481 atatacaagt aagtaaagac aatgttatac aattgataga tgaaattata aatagttata
    27541 acatatccaa aatagacact ccttataagg taattggtct tactttggct atacatggga
    27601 ttacttgtga aaataaagta ttgtttactc catattacaa tcttaatgag attgacttat
    27661 atagcatcct tagcaaaaag tatgactttc ctattcatat agaaaatgaa gcaaacttaa
    27721 cagctttagc tgagaatact ttttcaactg tacataactc actacttagt ctcagtattc
    27781 acagtggatt tggttcaggc attataataa ataataaatt atatagtggc agaaatggta
    27841 tgagtggaga aattggtcac actatcatca tgcctaacgg taaactatgt ccttgtggaa
    27901 atcgtggctg tttagaacag tattgttcag aaaaaaaggt atttgaacag ctttcttctt
    27961 tagaaaatat tcctaaaata gattctgata tagtaaagca actatattat gaagataacc
    28021 ataatgccaa aaaagttatt catgagtttt gtagttactt aacaatagct ataaataatg
    28081 ctattacaac ttatgctcct gaaataatat atttaaatag ccaaataatt agtgatatcc
    28141 ctgagatatt acagattaca aaagatatgt tagttagctc ttttaacaaa ggaataaata
    28201 tagaaatttc atcattaggg tctgaggcat ctctatatgg tggctctgct gtaaatatca
    28261 agagcttttt gaatatacaa aatttaactt taattaatga aatataaggg ttatgactat
    28321 atacagccat aaccctttaa attatacatt ctaaaattag atttattata tagtttatgc
    28381 aagattagct atatttttta aaaattgtat atcaattaag tattaacata agtatttcgt
    28441 tttaaatatt aatataatct tcctactcat catcagataa taaatttgca ttttgttcaa
    28501 ccatagaaat attatcttta cctattttct ctaattttgc tgccaattcc tttgtatcta
    28561 aaaaagctga ttcaataacc atacctaaat ttagccctga aataatttta attcgactgt
    28621 cattattggc tactaataaa gcagcattgc atggagttcc acctagtaaa tctgccatta
    28681 taattatttc tgaactttcc ttgtacactt ctaaagcttt ttttaatttt tctattgttc
    28741 cttctattcc atcatcttgt ttcatattta cagtaggata atctatagaa tcccctatta
    28801 tcatttttgc agaatttaag acttcttcag ccatattgcc atgactcatc aaaataatac
    28861 tattcaattt aatacacctc tttaaatcta taagatgtct acaagataag ttttcttatc
    28921 atcaggcgtc attatagttg taatttcaat tccttcatct aaaagtcttt taaatgcttc
    28981 tttctcttct tcagtaacac tgatattagg tcttagaact gttgtatctt ttctacttga
    29041 catatttcct acatttatat tatgtatttt aaatcctgcc tctacaaatg gaataaggtc
    29101 cacaggagac ttaacaatta ataatacatt ttgtccttca tactttcctg cagtaatatt
    29161 ttttatagca gtctcttctg taattataga tgacgctata cctgcaggtg tagccattct
    29221 taaaacttgt ttttgaacag aattgttagc tactgcatca tttactacca ttaatcttgt
    29281 aacacccaat ttattactcc acatagttgc aacttgacca tggattaaac ggtcatctat
    29341 acgaatattt ttaatacctt tcatttccat atctaatatc atcctttcct tattttaaaa
    29401 tacctaatgc atataaaatt attgaaacaa ccattactcc taaaattaag tatataggtt
    29461 ttatattctt tttaagagcc caataaacaa tcacaactaa aattgcagga actaacccag
    29521 gcataatttg atctaatatt tcttgacctt tcattgtaaa atcaccttgt tgaaagttca
    29581 aagcaacatt agcttttaca actgaaggta ttaatgcacc tacaaccatt aaacctaaaa
    29641 tagatgtact ctcagttaaa atctttaaac ttttttcaat tgaagatatt agcttagtac
    29701 ctgatgtata accagcatta aatagtggta tacttaataa tttcaatgct acactcacta
    29761 caatccacag taagattcca aatggacttc catctatcgc catattagct gctatagctc
    29821 caaaaattgt ccacggaata acgacaaata aactatctcc taaacctgct actggtccca
    29881 tcaagcctgt tttaattgaa gcaacagcct cttcagtctc ttcacttgat aactcctcca
    29941 ttgctacatt aatacccaaa atataagggg caacccaagg attagtgtta aaaaattgta
    30001 aatgatttaa tacagcactt tgtaactttt ctggattatc tttgtatact acttttaaaa
    30061 aaggtagtac tgcataacag tatgccaagg cctgcatctt ctcataattt gaagaaacag
    30121 aactagtaaa aaaccagcgc cataagacac tttttttcac cttttcattt gatttattta
    30181 atgatacctt atcactcatc ttcgtccata cctcctacta cagtatattg ttgttcttga
    30241 ttttccaaat tctttttgta aattataagt gcaattgctc caccaattaa agctacacct
    30301 aataatggta ctttcatata tactgctaaa acaaaaccaa ttactaagta actaaaatac
    30361 tccttagcag gaagatatct aagtaataat cctatcccta ctgctggaag tagtcctcca
    30421 gctactgtta aaccacctgt taaccattca ggtgtatatt tcaaaatggt attaattaaa
    30481 gaaggtccaa atattacaga tagagcaact ggaatacctg ttgtagctgc tgttaatagt
    30541 actcctaaca tttgcatata ggttatattt ttataatttc cttttttagc tccatcttct
    30601 gctttatgaa ctagccatat accaatagta tttctaagaa catctaattg taccatcaat
    30661 aaagcaactg gaatacctat agagattcct acagatgctt cttgcttagt cgtaatagtt
    30721 ataaatgtgg ctactatact tgctgtttga taatcaggta ttgatgctcc accaaagtta
    30781 ctaataccta atgataatag ctgtaatgta cctccaatat ataaccctgt atttatatct
    30841 cctaagataa gaccagtaat aaaacctgct gttactggtt gatatgttcc aaacattgtt
    30901 gaattttttt catagtttat aaaaaatcca tatagtacga ttaataataa ttgccataat
    30961 tgtggctcca ttatgatttc acctctccca aaatattttt tagttcttta aaattataac
    31021 tggaatcttg ctttaggaaa ataggaattt cttccagtgg gcattcaact tcataagttt
    31081 tcccaccttc ataactaact ccactaaata tattttccca tgtatgtcct tttggtaaat
    31141 atacttttct acttctatct ttataattca ttattggagc tactaaaatt tcatctccaa
    31201 gcatatatgt attatccacc tcccaagttt tatcatcttc aggaaattca taaaataaag
    31261 ttctcataac tggtgttcca tcttcatgcg cttctttcat cagttttttt aaataagttt
    31321 ttaagctctc tctaattttt atgaattttg taagaattac ttcaacttct tcaccataag
    31381 accatatttc attaggagct ccagttacca ttgagcctcc acctttattt gataatggtt
    31441 tactatgagg caatctatct ccatgcattc tcaaaattgg tgaaaaagta gcatattgga
    31501 accatcttac cataagttct ctaaattctg ggtctttagg atttcctcca tggaaacctc
    31561 caatatcagt agtccaccat ggtatacctg ccagtcccat atttaaccct gtatttactt
    31621 gattattaaa agcttcaaaa ctagaatcaa tgtcaccaga ccatactaaa gctccatact
    31681 tttgagctcc tgcccaagct cctcttacaa gagttacaac agactctatg tcattatgta
    31741 aaccatcata aatcattttt aaataatcaa taggataaat attaccacaa cttaaaacat
    31801 ttcctttttt atatctatag ttatcaaaat catacacaga atagccaggt tcagctacat
    31861 ctaaccaata atagtcaatc ccaagttgtt tataattttt atctattcta tcccaaacat
    31921 attctcttgc attttcattt gtcatatcta caaagactgt atttccttga atttgcattg
    31981 tcattctaac accacgatta acattgacta aatagccatt ttctaaataa tcattgtagt
    32041 tttctgcatc actttgaact gtaggccata cagatactat tggttctact gaatactctt
    32101 ctttcaattt tttaactaat tctttaggat ttttccaata atctaagtca aatttatatt
    32161 caccttgctt aggccaatga aaatagtcaa ttgcaattga tgaaagctta attcctcttt
    32221 tagaatattc cttcacaaca tctaaaactt cttctgaagt tctatatctt agtttacttt
    32281 gccatagtcc taaaaggttt tcaggcatca taggaacagt tccagtaact tgagagtaat
    32341 ttttatttaa ctcttttggg ctttctccac aagtaatcca ataatctata aaatttgtag
    32401 aaaacatctt ccattctgtc atatttttag caaaagatac ttttccaatt cccggattat
    32461 tccataaaag tccataacct agactagaaa tataaaaagg aactgatatt tgagaatttc
    32521 tttgggctaa ctccaaaaca gtattcttta aatctaaaaa tttatgttga tattgtccca
    32581 ttccaaaaat cttttcagaa ggctcagatt caaatctagt agttacttca aattctccac
    32641 tatagcttgg tcgatattct cttgatttta actttagagt agagttaaag tctttggtaa
    32701 tctcaattgt ccctacatct tctccaccat catcatgttt tacagctctt aatcgaatat
    32761 attctttcaa taatatttca tttttatcat tataaaaagt aattctatct ctatggtcta
    32821 agacagcttt tatctttcca tttttaatga ttgcacttcc atcttcattt ttattaattg
    32881 taatatcatc tttatttaac ttaggttttt cgttaagagc atagttttca tcaacaaaat
    32941 tttggtctac aaacgaccta atacgtaaag agttttttcc ccatgcttct attcgcaata
    33001 gttcattgtc aaatcttttt tcaatatagt cttcagatat ttttatcacg gctacctcct
    33061 aaatcaatgg tataatattc tatctttgtg taacttaagt taaataatgt cgtttagtat
    33121 cgttatttat aaattaggtt ttatatataa ttcatttgta cattaaacta acttaccaat
    33181 aatatacttt atttgacttt gtttgtcaat cgctttccgg attttagtaa aattttctaa
    33241 tatattgctg gaaagttgtt cttaatatct tttatttata cttttaatat ctgtgatgat
    33301 acctatatac atctagtttt tttaaaacac atacaggaat aacatttgcc tattatgtag
    33361 ataatatcgg acttgaaaat atgtatagtg accttataaa tacagataat aatatattag
    33421 atattgttca aaacaatgga ttttataatt ataaataatt tatgaaatta tttaagaaaa
    33481 tatacaattg tataccaagt caaaaacgac aacaaataaa taaaactaaa aaagatgttg
    33541 tctcaaaata tagataaatt tatttttcat tgctaaaatg attaaaatat atttgattta
    33601 atatacttga tttaatataa aaaaagagtg tctcataaac taaaaagttc acttttagac
    33661 actcctttat atatattatc tacttcttaa ttcatttatt acctttctac attttctttc
    33721 agctagtgga tacatcttta taagtactaa ttactccact taaaaatggt tcctgtactg
    33781 tccatcagtc atatttttct gccaaatata atggtgtttc ataatgagac aataaatatt
    33841 tatatctcta cttatcttct ctaagctcgt caaatccaac tataggtatc tctaaattat
    33901 attctttaag tagcttttct agctcttcta taaaaatacc tttcccatcc accatttgta
    33961 tatcctttat tttatccatc aattcattgc aaccacttaa ctcaccaaac caacgtttag
    34021 aactacatgt cttattataa ccacaacttg gactgccatc tattgcaatg atacttttta
    34081 tatcataatc attttttaaa tattcatcaa actgccttac atgaggtatt aacatttgcc
    34141 tacattgttc tctaaaaaac aaattgtcaa actgttcttt tacatgtccc cacctattta
    34201 ttccatacat aatcatttct gggcatggta gttgaattat tccataccct ttatttatta
    34261 aatttatcgc tatattttgt gctcccttaa attctgacaa tccctccact ttagaattac
    34321 aatttaatat acaatgacat actactacaa tctctctatt tcttttcata ataatctcct
    34381 tagttattct taattttaat gatactacat attttgataa ttatattatt tatttgttct
    34441 atatttccaa tattctatat agaaatatac aattagtaat cttttataat ctatactatg
    34501 ataaatgtat atattttttt aggaggtatt ataattggat actaaaaaag tcacattttc
    34561 ttcaatgtgc atagttatta atattgtctt aggtatggtt atatctatga taaatatacc
    34621 attattattt ttagatacag ttggtactgt acttggggct gtcgtattag gtcctttttg
    34681 gggtgctctt attggtggat gtacaaactt agttttaggt gttatatcag gtcctacaaa
    34741 tatacctttt gcccttgtaa atattgtcct tggtcttatt gttggatata tttctaagaa
    34801 aaaaggtttt ggttataaag aagctattat tacaggagta attttatcta ttgtttgtcc
    34861 tttaattggt actcctattt cagttttatt gtttggtggt ttaagtggaa gtggtgctga
    34921 tttattggtt ggttttttag ttcagtctgg tcagaagata tttactgctg cgttctttcc
    34981 aaggattctt agtaatatag tagacaaacc tttgtcttgt attatggtgg tatattttat
    35041 aagtaaaatg cctaaaggtt ttataaaaca atatagtaaa aatacttcta gttcatatta
    35101 ataaatcatt tattatgtaa ggtttattac tctttaaaat gctatatact catttttata
    35161 taacattttt attaataatt taattagata gtatttgtat tactttaatt aaacgtaacc
    35221 tcttgatata aatttgagct ataaatctat atcaagaggt gctattttat ttttaatata
    35281 attaatattc tattacgcaa aaaatgcata tatttcatta ttttatggaa aatattactt
    35341 catttaactc tatatcaaac tcattaagta aaaatctaat tatatttaat ttagcctctg
    35401 tatttaaatt tgtttctaaa tatatatttt ctgatatttt aatacctttt cttaatttat
    35461 atgcatctct agacttaata ttatctataa aatctctttt ataagttttg tctacaatac
    35521 tgttaaacct tacagaatcc aaatcatata aagtaagagc aacttgttca taaaattctg
    35581 tccaattttt agccttatat gactctccta atatactaaa tgatactata ctttcatttg
    35641 taaaattaac atcatcatcc tctaacgtaa atatattttc actgttactt ggaggttcat
    35701 atgtaacact tggatatttc caaatttcta cagctctatc tagtaatatc ttagttctat
    35761 tttttatttc ttcttcattc catttatcta tagatgaaat atatttattt agataaagcc
    35821 tactatcttt aaatccatct tccatttctt tcttttcttc aaatgtttta ttacttagtt
    35881 ttgaattata tcctgtcaaa gttaaatttc caattgtatg taaatattta tcatgtatct
    35941 ccttatagtt atctcctagt gattccttcc atttatttgt taatgtctgt ggcattatat
    36001 gctctatatt tagcatatta ttattgatta aattttctaa atcaacaatt tctttattac
    36061 tataattctc taacctctca agtatatata acttattctt tgacttagta ttatatatat
    36121 ctttagtttc aaaagcattt gaaaattcac tgtcagtagg aaatctttga gatgatttct
    36181 tatttataaa aatatatctt aatatattag aatagttttc tttataatca ctaaattttt
    36241 taatttccct tcctaagttc ataaatactt tattcagtgc attcggaggt acatcacata
    36301 ctattcttct taatataaaa cattctacta aattaaggga ttctacaagt acttttttat
    36361 ttattatacc ttcattataa tcatcaaata attctaatat aaacggatat gatacaaata
    36421 tttcaagttt gtttatcctt ttcaaaactt tgtttacatc attatcatca gtacatgaat
    36481 aagatatctc tctataatag ttagaaaatt taagtaaatc ttttaataaa atttctatat
    36541 ctataccatt atctgataca taccttttaa aactaaaata tactttattt ttattcggta
    36601 tacttctctc ctttatagtc aaatagtctc ttataaaact acttacgttg ctacttgtat
    36661 ttttttcaat tttataccaa taattattat aaaattcctc ttgtaactta gagcttctac
    36721 ccattaacac aaagtttctt actctatcag cttcacttaa atctaagcca gttgaattta
    36781 gactttcaaa gattaattga gggtcatctt caccattttt taactctatt tctactatag
    36841 ttaatttctg tatagcatca aacaaatcat ctattgatat attatcttct tttattctat
    36901 tataaaaata taaataattt gaaactatat tagaatttct atctgtattc atttcttctt
    36961 caaatagttc atagtacgct tttctatctt ctgctattgg ttttaacttt atttttccat
    37021 ctttatctct tgagtgcttg tttataagat aacactcctt aatctgttct tctgttgtaa
    37081 taattccttt tgattcccca aactctttta ttatttgaca catggcaatc aataatattg
    37141 acacagtagt cactctttgt tgtccatcta ttattaaata ttcttttccc atactatata
    37201 tagatacaac tgaccctaag aaatgagttc taaatccaga atctattata tcctctaaat
    37261 catcaaacaa tcttttacat tgttctttct tccagttata atttctttga tatacaggta
    37321 ctacaaaact tttgtttgac ccctctaaat aactaagaaa ttcaatttct cctgctttca
    37381 tattaactcc ccctataagt taaataaatt ccattcataa ctccatgtat ctatatatta
    37441 tatcaaaatt ttgactcata aatgtatatg aaagccttat tatatacctt tttatacctg
    37501 tttttatttt attaaagtaa atacatgatt attctataaa atatacaaat acttaatttt
    37561 aaaaattatt atttaatctt cttcatgagt aattcatagt tatttatcaa ttaaaaagag
    37621 atattgcata attattcaat acctctcttt gtatatttac attatccata tctagttatc
    37681 tatttcaacc caatattaga ctacctacta ataaatttat tctctacatc attaacaata
    37741 attttgcact aaataacatc attagcctat ctctgatgca ttttctttaa ttttatttat
    37801 agttttaatc aatttatttc tctcttcaat atgattttca aataaatcaa ttatacttcc
    37861 aacagttcta aatgggtctt ccatcaacac tttgttatcc tctataaatc cattcttgac
    37921 tacataatcg actataagtt taacaaaatg tatttgctta gtattaaagc tcttattatt
    37981 taaaaactct gaaaaagctt catttgcagc ttctctatcc aatcccacta gtcttctcac
    38041 taattgactt actggagtat ctccaaactc cttcttataa tcatcacaat tacctaactg
    38101 ttttaagagt atttcctcca acgtttcaac atcttgcttt gttatctttt tattatttct
    38161 taatttaaat attgctaatt catctttatg gctatttaaa taatactcta ccttcttttt
    38221 ataattctta agattgtttg cattatatac agaatcattt ctatcttctg ctacaatcat
    38281 atcttcaaaa cttgtataat atatcttttg actagacttt tcaagatact taataagctc
    38341 tcttaatgat atacgtactt cttctacctc aaatatatct gaatcttccc aaaaattctc
    38401 tgtcattgct ttttcaatag tatatttttg ttgctgaatc tgtggtatag tcccaagttt
    38461 tgaaagtttt tctgctgtag ccattacatg ccttatacct ttacttgcat tattattttg
    38521 taagtatgca agttgtatat tatacataac tacatcaaat cttttagcca attcatcatc
    38581 actatatgaa gttatgagtg gtgatatata ttctcttatc tcattttgtg ctactgctcc
    38641 aatattgctc caaacatttt catttttata cttatgtaca taacttaaat tcatcctaac
    38701 tatataacta tcctcattta aagcttttac agaagttata agttcttcta acaattcttt
    38761 tctaagttct gcatactcac cttctatata tttaatatct tgaagttctt taaccatact
    38821 tacctttata ttaaataatt tttcagtaag tgtttctgct ttatttccct caaatccttt
    38881 aggattaaat ttaaaaaatt caaaattatt acaaaaatca aatattaaga atctctcctt
    38941 atccatatca acacctaata aatctggaca taatctagtt cctctaccta tcatttgcca
    39001 aaacttagtc ttacttctca ctttcttgaa aaaaacaaga tttagtattt caggtatgtc
    39061 aattcctgta tcaagcatgt ctactgaaac tgctatttga ggtaatttat tcttatcaga
    39121 aaaatcatct attatagaat ctacataatt tatactatag tcaactgctt ttacaaagtt
    39181 cccaccatac tttggataca gcttattaaa tctttcaact attgccctag catgctttgt
    39241 attctttgca aaaattatag tcttaccaat cttttcttct ccttctattt tcaacccctc
    39301 tgtcattagc ttatttaaaa ctaaatctat tgtgtcagaa ttaaatagcc actcatttac
    39361 tgcattattc cctatttcgt ctccaatact ctcatcatca ttaaatgtat tttcatattc
    39421 ttccttctct tcatcagaaa gctcatcata cttaattcca tcttccataa tcttagtctt
    39481 aaactcaata ctagtataat ctactaaata tccatcctcg actgccttat tatactcata
    39541 agcatatgta ggtactccat tttccatgtc aaaaactgaa taagtatttt tatctatctc
    39601 atcctttggt gtagcagtaa gacccattaa gtaagaatca aaataatcaa agattgactt
    39661 atacttttta taaatacttc tatgcgattc atctattata attaaatcaa aatgaccagg
    39721 tgtaaacaat ctctttccat ccttagactt cgtatcatca attgcattca tcattgttgg
    39781 atatgtagaa aatatcatcc tagcatcttc tgggctgtct ttgctatcca ataaattaca
    39841 taacgccaag ttaggtaata gcttagtaaa acttttttta gcttgcttta ccaatgcttt
    39901 tctatctgct agaaataata tatttttaat ccaattatgt cttgaaagta catcaactat
    39961 agatatagca attcttgttt ttcctgtacc agtagcacat accaaaagag ttttgcgttg
    40021 cttattttca agggcctcac atacagcccg aatagcttct ttttgataat atctattgga
    40081 tatttcatca tttattgtta cagaacttaa agacttctta ctctttcttc tatccatcat
    40141 aagttgtaat tctgattttt tataaaatcc ataaactttt ctatctgaat agtcatccca
    40201 aatatacata tcaaaaccat tagtcaaaaa tattactggt ctttgactat atctcttttc
    40261 caaacaatct gcatataatt tagcttgttg ttgaccaatt tttgggtctt tgctcgttct
    40321 tttcgcctca actactgcta atggcttacc attttcacca taaagaacat aatctacata
    40381 tcctacacca ctttcattag gcataccttg tacttctatt tcctccccta tatcttttcc
    40441 aaattcccaa cctgcaagct ttaattctat atttatatat ctttttcttg tctcaaattc
    40501 gcttacttcg tctatcttga aatcatagtt tacagtatta acttttctct tttcagttag
    40561 tgactttctt aattcctcat tttcttttat tacttcttca agctttttat cttttgaact
    40621 taatctctcg taaagatttt taagttcgtc tggtctcgtc cttttctctt ctccatgaag
    40681 taataaatct tcattaaagt cttctgctgt atattcatct gaataacaat agtctatcca
    40741 actaacaaat tgatgcaaat tgttaagtga aagtattgct tctcctctct ctatgttcgc
    40801 actagtatgt acagataaat tacctaactt aactatatat tttaataaag gtaacatatc
    40861 atagtctata agttctataa aactattatt atgaatcaac gtactgagat tatcttgata
    40921 aggcaatact aaatcactat cattagcata tagccatttt actgcaagct ctaaagctct
    40981 tcttgttata gttgcacaag tagatggact aactagtata cttttttctg cttctaagca
    41041 agcagtatta aaactattga aatttttctt cttttgtaaa aaatcaaaat tacccaaggc
    41101 ttttatcccc cttttgtccc ttagttaaaa taaatctctc taaaaactat ttcttacagc
    41161 tgtttcttat actaacaaat acttcttcat tttctgtaaa caattcgtac atatcttcaa
    41221 actgtggtat atcatatcca tgagaatcat catttctcaa ttttctaagt gtttctataa
    41281 ctatagaaga atttttaggt aataaattgt gatactcaca agctaaaagg tagtcataaa
    41341 agtttttaga ttttatgttt aaatcaattg atttataata caacattgct tttgttaatt
    41401 ctagaaatga taccatatag cttttaaagc cttcttttat gtatctagcc cataactcag
    41461 gcatttcttc cttatcatat aagattttga agttttgcag gtcatacaag caagaaccaa
    41521 aatccctatt tacttcttct attgagttcc aagataattt gtttctaatt accatacatg
    41581 tccctccaat ctaagatatc tattacatca ctgttttcaa aatagaattt atttatataa
    41641 tccttaaaat ctttagattc ataaataact tcaccttcta aaatagcttt tttaagtgct
    41701 attgtataat tatctgtaac aatttttagg tctacatcaa tgtctaatat atttcttaga
    41761 gcttctgtct tagttgcaag ttctgtaatt ggtacttttt tgatgggaat ccaagctatg
    41821 tctatatcac tttctgatgt atgtttatct gttccataac taccaaatat tatgattgct
    41881 agagtatttt cttttggaaa tacatttatt attgagtttt taatctcatt tggtatattt
    41941 ctcatttaat cacctcttgt ttattaggta atattgttta gttaaataat tctcctttga
    42001 aagctttttg cattaatgaa ttaaagttat cttctaactc ttttaagttg gtttctattt
    42061 ttaattttaa tttatccact tgcttaacaa actcagaaaa ctgattttga aattcaattg
    42121 gaggaaccat tattaaatat tcacctaact tactaactga taactcaatt tgattcgttg
    42181 aaccattaac acattttcta tatattgtgt cttgtatatt tgtaaattca aataacgctg
    42241 caaaataatc tgaatttaag ttatttgaaa tacttcttaa aactgtaaca tgactatctg
    42301 ctatatattc agaattatca attttgttaa attttacaca tcttcctaat gtcccagttc
    42361 cagtagaatt tattaatata tcattttgtt tcaaaaatat atcttctatc ttctctttta
    42421 agttttcatc ataatattta caattttcta ccttcaaatt tctccagtat atacattttt
    42481 gatttataac ttttatattt gattgctcta catatttagg agatacacct ctttttaaat
    42541 atatagaaat ctctttaatc ttcttcatat caaatttttt tgtattatta attggctccc
    42601 caaacatctc aataaatctt gatttaacta attcatccaa tgcttctatt tgctcttttc
    42661 ttttatttat taactcttga gctttattta aaacttctac cattttattt tgtatacaaa
    42721 ttgtgaaata tggtattttt atatcattta acttgctttt atttaaagtt gcacccataa
    42781 ctgctctatc agtattatta aaatttaaag ttctcaaagc ataatataaa tattcagatg
    42841 ttataagttc attatttttt atttgaaagt tagcaattgc ttcatttgta aacatgtctt
    42901 cttttaatat ggcaactcta cctatagaca acttaaaact cattacaacg gtatttttag
    42961 gtactaattt catattagct ttttcaacag ctaaatctgt tatctcttct tttgattttg
    43021 atatatattt ttctttcaaa tcagaaattg ataaccatct attaccactt ccccaataat
    43081 cagaagtatt tctagatggt gttttgccaa tatttatata gcacaattca tttaatttta
    43141 tatactccat accctatacc tctaacattc tttctaattc ttctaatcca acagctattt
    43201 ccttctctaa ctccctaact ctacctataa tcacactagg agcatcataa actacttcct
    43261 catacactac ctctttatac ttattaatag ataaatcata cccattctct cttatctctt
    43321 ccacatcaac aaaaaagctt tgttctgttc tttttctatc tacttcccca tcaagattat
    43381 caaatctctt aataatatcc tctatatcac tatcctttat ctcatttctc ttatcatcca
    43441 aagaataacc atcagctttc atatcataga accatacctt atcagttcca cctgtccctg
    43501 ttttagtaaa tatcattata gctgtagata ctcctgcata aggcttaaac actccacttg
    43561 gcattgatat tatagcttcc aatttatgat tatcaactat ttcttttctt atcgatttat
    43621 gtccacctgt tgaaccaaac aatactccat ctggcactat acatgcgcat cttccaccaa
    43681 tttttaaaac tcttaaaaaa agtgccaaga ataataactc tgttttcttt gtcttagtca
    43741 cttttaataa atctgctgac actgcttcat aatctaaact tcccttaaaa ggaggatttg
    43801 ctaacaccaa tgtaaactta tctttatctg tattaacctc cgataaagaa tccttatact
    43861 ctatattagg attgtcaacg ccatgcaaca tcatattcat agcacctatt ctaagcatag
    43921 ttctatccat atcaaatcca tagaacatac cattattaaa atgctccttc agcccttgca
    43981 ctaagaaaag acttgaatga ttatccctta agtattgttg tgatgatact aaaaaacctg
    44041 ctgaacctgc tgccgggtct actataatat cttctggtgt tggtttaata agtcttacta
    44101 tcatatctat tatgtgtctt ggagtcctaa attgaccatt agtacctgct gtagccactt
    44161 tggataataa atactcatac aaatctccct ttgtatctct atccttactt aattctaaat
    44221 tactaatacc atcaactatt ttagataaca taagaggcgt aggaatttta aatattgcat
    44281 ctcccatgta ctttgcataa gctgagtctt tatttccatg taattttttt atgaaaggaa
    44341 acacttcatt ttgtactatt tcatacattt tttctgaatt tccttcatta gaaaacttac
    44401 tccatcttaa atgttgatgt tcatcatcaa atatcctttc aaaagaaact cccaaaagag
    44461 ttgcttctgc ttcatttttt gtttctactt catccaatcc tttaataaat aaaagatatg
    44521 taaattgttc tataacttct aatggattag ttaatccacc agtccaaaat gtctcccaaa
    44581 tcttgtctac tttgctcctt aattctcctg taatcatata tgactttccc tccagtgttc
    44641 ataaaataat ttaaatatat attttagtcg tacgccttaa taattagtgt cttatattaa
    44701 attcaaaatt ttactacaca gttattaaat tatttttcct atttacatgt tttagttaaa
    44761 aaactaatgc aatactctta ttttatgatt attatacatc ccatacaaaa ttattttatc
    44821 atatatccag catagaagtc atctacctgg ataactctta ccatattata actttttaat
    44881 attaatttaa acttagaaag aaatttttta tttaattata tcatcttcct tatttcaaat
    44941 ttctcaatta caatctcaat caaaaaataa aataagatgc tgtctcaaaa tagatataat
    45001 cttctttttt attttcggaa tgatgaaaat atatttaatt tcatacaaaa aagagtgcct
    45061 cctgaactaa aaagttcatt ttgagacacc ccttaattta atcttactta ttctctccct
    45121 ttattattaa cacttcacta tctctattgc ttttcctaca catccacaaa cttccatcct
    45181 actcttaaca aaatctttaa ctgactctaa acctttttta ccatatttct tagggtcaat
    45241 tacctctggg tcagaattaa gcacatcctt aaccccattg ctataagcaa tccttaattc
    45301 agttgcataa ttaaccttgc aaatccctct ttttatagat tccttaataa cttcatctgg
    45361 tattccagac ccaccatgta atactaatgg aactgataca acatctctaa tttcagataa
    45421 tctatctaaa tcaagctttg gctctccctt atataatcca tgtgccgtac ctattgctac
    45481 tgccaaagat gatacccctg ttctctctac aaactctttt gcttctaatg ggtctgtata
    45541 tgctccccca tcaccaccat ctaaatcgtc ttccttacct ccaactttac ctaattcagc
    45601 ttcaacaggt atatctgaag gcatacatgc atctacaaca gatttagata ttgaaatatt
    45661 atcttcgtaa ctttcatgag aaccatcaat cataatagaa gtatatccaa ctctaagtgc
    45721 ctgcatagct agtccaaagc ttgaaccatg gtctaagtgc attgctactg gaacacttgc
    45781 cttctctgat gctaccttta cattcgctaa ataataatct agccctgcat actttattgt
    45841 tgaaggtgtt gtttgcaata ttactggtga tttaagttct tcagcagcct ctattactgc
    45901 cataaccatc tccatatttt caacattaaa tgcacctact gcataatgtc cttcttgtgc
    45961 atctaataat atttgtttag ttgtcactaa tgccataatt tctcctccta tatgtttata
    46021 ctgatttgat ttcttcgtta tttaattttg ctactatttc tctacttgtt ttgctattta
    46081 ataactcatt taaaaattct tcattcatta attttgaaga tagttgagat aatgttttta
    46141 agtgtaaatt atcaccatta tctgtagctg ctatcataaa tataatatgt gctaagcttc
    46201 catcttctga ttcaaagtca aacccttctt ttgatacacc tactgctaca cgtggtacct
    46261 ttactgcatc tgtttttgca tgtggtatcg ctatccccat accaaaacct gtagatgata
    46321 tttcttctct tttaaggatt tcttctttaa acttcttttt atctaaaaga acactagatt
    46381 tatcaagtat tccaatcatt tcatcaatta catctgtttt attttttccc ttaacatcta
    46441 aaattactgt ttcttcgcta tataaagaga tattattaga tacgttttcc gattctgcac
    46501 tttcattttt atgttcatct tttgttacat ttgtattttc tatagtattt ttctcattta
    46561 ctactttagg tgtaaccatc atataaagta cagcacctac taatgaacct acaaatatac
    46621 accctaccca taaaagtggc ttattgacta atcctagaac caaaaaccct ccatgtggag
    46681 ctggtacttc tattttcatt atataagtta aaattgatga tattgatgat gcaatcataa
    46741 gtactggcaa cacttttaat ggattttttg ctgcaaaagg tattgccccc tctgttatat
    46801 gagtactacc tagtatataa ttgattaatc ctgctgctct atcttcttct gtaaatctat
    46861 ttttaaataa agtagatgct attgctatta ccaatggtgg tgttatacaa gctgcagata
    46921 cacctgccat aaataaataa tttccctgtc ctagaagcat agttccagtt acatatgctg
    46981 ctttatttac tggacctccc atatcaaatg ctgacataca tcctattact attcccaaaa
    47041 ttatagggct tgaattttgt aaccccgata gaaagttcat catagcattg ttaagtgctg
    47101 aacatggccc accaagtaac aacattgcta cacccacaag acctacgctg acaataggaa
    47161 ttataaaaat cgatttaaga ccttctaatt gtcgtggtaa ttttcttaac aaatgtacaa
    47221 atagtttaac taagtaacct gctaaaaatc ctgatatgat tccacctaaa aaccccgaac
    47281 ctgtatcaaa tgatatcatt cctccaacaa gtcctgctaa cataccagct cttcccgata
    47341 tagattgagc tataaatgct gccaatacag gaaccatcaa gttcatagaa tatccaccaa
    47401 cagattttaa tgtagctgct atttgattat attgttctga atttgggtca aatgaataaa
    47461 tcccccataa aaatgatata gcagtaagta ctccacctgc tactactaaa ggtaacatgt
    47521 gagatacacc attcattaag tctttataca acttttgacc aaacgataaa tttccttctt
    47581 ctgcaatttt actgcttgat aagtttcctt ttctaatagc tacttttcca tcaatacatc
    47641 tttgtataag ttcattagct ttatgtatac cttctttgac aggtacttct attactggct
    47701 ttccattgaa tctgtccata tcaacatttt tatcacaagc aactattatt gcatctgcca
    47761 tttcgatatc ttttgctgtt aacttatttt ctacacctat agctccattc gtttcaactt
    47821 ttatgtcaca ccctaattcc ctggctgaat tttttaatgc ttcttctgcc ataaaagtat
    47881 gagctattcc agttggacaa cctgttaccc ctagaatcat tttctttccc atttttatac
    47941 cacctttctg actcttatat tttttatata atcttccaca ttctccaaat tacctatacc
    48001 attactttca gccacacttg ctcctgttgc tgctgacctt tttaatgctt cttcaatatg
    48061 ttctggattt tccaaccata tacttaaaaa agcagctaat gcagaatcac cagcacatgc
    48121 agagctgact actgtaactg gttgagcact agcataataa attgattttc cattataaaa
    48181 ataagaacct ttttctccta aagttagcaa tatattttga gcacctttta aatgtagtaa
    48241 tttaagtaca tccttaatat cttcttcatc tcttacaata attccaaata tatctttgat
    48301 ttcttcatca tttggtttta tcaaatatgg attatactgt agtaactcct ttaattttgg
    48361 tgaacttata tcgagtattg tctttatact tttattttta caaattttaa atatatcctc
    48421 atagtaatta tcatcaatac caggaggtag acttccactt attgataagt aacttatatc
    48481 tgctaatgac tgtatcttat ctagcatatc tagtttttgt ttttcattta catatgaccc
    48541 agagtttaca aatttaaatt catcacttcc atcatttaaa aatatattta tacgagttgt
    48601 atcttctacc caagtaggca atacgtcaaa tcctctcttt tcgctttctt caactatata
    48661 ttttcctgaa aatcctccaa aaaatcctaa tatcttagat tctataccat aatgacctag
    48721 tacaaaagtt acatttaatc cctttccatt aggagaatat acagcatttg atgtacgatt
    48781 tactaatttt ctctttatcc cattagtaga tatattcata tctattgcag gatttgtagt
    48841 taatgtatat atcattgtct tcccccttgc ttgtgctgtg tatgttttca ttataataaa
    48901 aaagtctatg aaaatctttt cacaaataga aaatcgattt tcatagactt ttcaatttaa
    48961 ttttcagaat tattatatct gaccatataa tctataatta tcctagatat aatatacaag
    49021 ggcaatctgg aagatacttc atattcagaa attgatatat gttcatgttt gtatcctaca
    49081 atatataaat ccgaatactt tataagtttt gcactttcac tacaacagca agttataacc
    49141 ttagctccaa ctaaattggc attctgtgct gattctacaa gctctatggt atttccattc
    49201 aatgaaaata taattataag ttcgtctttt tttattgatt tagattttat tttcattatg
    49261 ttggggtcat ttataaaaac aacattaaaa tcaagtaatt gcaatttaag actaaactct
    49321 tttgctacat attcagtaag ccctcttgct aaaatatata tcttactaga tttatttata
    49381 gtttttatta tattgattag tgtagttaaa gatatttgtt ctataactct ctgtgcttct
    49441 ataaaggatt tatttattat atcccccata ttttgtatat catcattttc atcttttact
    49501 gttaatctat acctaagttc attgaatcca tttaaaccac attttctaat tgctcttgaa
    49561 acggttgctg gagatgaata tgtttcatga gcaatctcta ctatagataa ttctggtaat
    49621 attgattcat tttcatttat aaatttaatt atttcaagtt ccgtcttcgt tagattttca
    49681 gaattacttt tgtttagctc aattatcata tgttatctcc ccttatataa gttttatttg
    49741 tctaaattta tcgagtaata taatatatta cctcttaact acttattttt atatacaatt
    49801 aagatttagt caatgtctat atatgtaaat ttataatata ataatttttt gcttaaagtt
    49861 tattatgtta tcttatagca ttttaaaata aaaaagaatt gtacctctac caaaatttgg
    49921 atgaagtaca atagatattc ctcatactga tatgtttatg attaaaagta taaatataaa
    49981 tttcgctatg aatcatataa ttaataaatc attttattta caattataac cccccttata
    50041 aaactatatt aataatatat acagtattta taaggggggt tgtttatatt taaaaactat
    50101 ttacttttct cttaattctt ctccattagt tcttattaca ttttgatacc aataaaaact
    50161 atctttttta taacgttctt tacttccatt tccattgtta tctatatcca catatataaa
    50221 tccatatctt ttttccattt cttgagatga acaacttact atatctattg gcgaccacat
    50281 acaataagct aatatttcgg ttccatcagc tatagcttca cctatttgtt ctatatgttt
    50341 tcctaaataa tctattctgt aactatcatg aacttttcca tcttcaaatt tatcatgcat
    50401 tccaaatcca ttttcagcta tcattattgg cttttggtat ctatcataat attgagataa
    50461 agtattgtat aatccttttg ggtcaattgc ccacccccaa ggatttgctt ttaaatatgg
    50521 attctttata ttgtcagttg gattcatggt gtttttactt gctgatactg ttgtagaata
    50581 atagtatgat aaagctagaa aatccatagt attttctttt aagattttat catcaccttc
    50641 ctccataact atatttatgt tattttcttt gaaaaatctc ttaatataga ctggatactc
    50701 tcccctaaaa cttacatcag tgaaaaaata ttgtagccta tttcttttta aagcaaatac
    50761 tacattttca gggtcacaat cttctggata agatgtacaa tctgccatca tcatacctat
    50821 catcatatct gaatttattt cttttccaag cttcttagtc aaagctgtag ctatgaattg
    50881 atgatgaata gcttgatatc tagtctcttc tacattatca acttggtcta cacaaaatgc
    50941 aactgaatta aatgattcat aatgtattaa atttatttga tttataacta tccaatattt
    51001 aactttattt ttatatctat ttagtattaa ttctgcatat ctaacaaaga aatctaccac
    51061 ttttctatta gaccatcctt tatattttaa tactaaattc aaaggagtct catagtgaag
    51121 tagtgttaca ataggttcca taccattatc tattacttca tttataagat tatcataaaa
    51181 ctttaatccc tcttcattag gctctaaatc atctccattt ggaaatattc tagcccaatt
    51241 tattgaaaat ctaaaacact ttaaccctag ctctttcaaa agctttaaat cttctttgta
    51301 tgtatgataa aagtctattc cactacgctt aggataatat ccattattat ctttcatagc
    51361 aaactttatt ctttctatag tcatatcaga ctcataaggt ttagatatat ctctatcttt
    51421 atcatacata tgtacatcag ctaaacacca acctttgcca ccttcattcc atgcaccttc
    51481 taattgatta gctgctacag ctcctcccca taaaaaacct ttaggaaact tacccaattt
    51541 aatcactcct tattttattt atttaaatca tttactatta tttaccttat attctataga
    51601 ctttactatt cctactaaag acatagttat atttaactcg ctgaatatgg tcataagagt
    51661 atcttgagca tgtgcaaata acagcgaatg tgctcttttc tctccaccag cttccccttg
    51721 tataatcttg gtttgagctc tatgtgcttc tgtcataaac ccttttgctt tagctagttt
    51781 taaatcagca tttctataat caaacttttc caaatcatta agagcatgtt taatttctgt
    51841 tctagcctca cctgcacgaa gtattatttg cattgctact tctactaatt cttcatctat
    51901 tatatgttct tcaattgtca tatttataat ttcctttcaa tttctaaatt taaacctctt
    51961 gactctctaa ttttatctct tctttctcat aagctttaaa aaatggatac catatcataa
    52021 atagcaatac tatatttatt gctaaaacaa taagacctct aacatcttga tttaccataa
    52081 aagtagatat accaaaagga acataccata aattaaacac cttacttgga attgtaacaa
    52141 atcccatact cattgataaa taagtagtta ttgatattac tactgttcct atccacatag
    52201 gtaacattaa aaatgggttc catgccactg gagctccaaa tattactggt tcatttatat
    52261 tcattataga agggacaata catgctcttc ctattgcttt tattcttcca actctggata
    52321 aacacatcat tataactaat ggtaatgtag cacccaaccc tcctaaagtt atccagcctg
    52381 tttgaataac ttcgtaaaca tatatattag ttgctactcc tcctgatgct actgctgctg
    52441 cattagctgc aattgcacca agttgaattg aataagttat aggagatagt acccacccac
    52501 ttattcctaa agaatatata aatactacta agaagtttaa gagtacaaat ccaggtaatg
    52561 tctgtgctat atctgatact ggtttaaata ctctaactat tatttcaaac atgtcaaagt
    52621 tgagtccata aattaatccc cagcctacaa acattatcac tgttataggt aacatattat
    52681 caaaccactt gcttacaaaa tccggaagtg ctgaatcttc tccgaaaaat gaaaaagaac
    52741 caaataaaga cattattaat ccagagaata atcctgcaac tagcgataca agcattcctt
    52801 ctcctccaaa cctgttgaag ttaaatatag ctccttcatt cgtaatctct ggacgaatca
    52861 tcattaacat taatccagca cctgcaagac cagctactac actcatactt tcaaatttct
    52921 tattcttcat tgtttccata ggcaataaaa atgctataaa taatcctaaa agtccaaatg
    52981 tatattgatt tattggactt aaatcaggta aaaagcttat gtaattcttc aagacagaaa
    53041 ctatagttac taatgagcct acaaatacca ttggcaaaac tttcattata gttgattgaa
    53101 ctgattgtac ccatatattc ttagtcatat tatttacaac aggagtaaat ttttcactta
    53161 aaaaatctat aaatttattc ataaacactc tccccataaa tattttattt gtttgatatt
    53221 agttctagtg cttcttccaa agcctttgca ccatctagtc ttccatatgc agttttagat
    53281 atacatgcta ctggaacgtt atattcatta gccttttgtt gaatttcttc cttcatataa
    53341 tctaaatgtg gtcctagtaa taaacaatct atttctgata aatacttatc tatctctgac
    53401 tcacttctcg cttttatatc tgcttctata ccttttttct ttgcactctt tctcatacta
    53461 gttgccataa aacctgtact tgctcctgaa ccacacgcca aaagcactct tatatttttc
    53521 ataataatac ctccaatatt tttatccatt aatttatttc ttaatgaact ttttatttta
    53581 ttatgtttat attttacttc ccaaataagt tcatttcaca taaatatatt tcctaataat
    53641 aggaaatcat ttactcttaa ttagacctat taatatagta taatgtatct tataatacgt
    53701 acattcaatt atataattat catatcatac agattttatt ttaaaactat gcgagggaat
    53761 ctaatgaaaa catctaaaaa agagctattt ttaaatttca taaaaacgca acatgattgg
    53821 atagattcat ccacattagc taattacctt aatgtatcaa cacgttcgat tagaaaatat
    53881 gtaaacgaga ttaattctaa tggtgcattt atattatctt ctaaaaaggg ctataaagta
    53941 aatctcaata ctaattgtca aactaagatt gatagttcag agaatatatc acctgataac
    54001 agattaaatt taattttaaa agaacttata gttaatagta atggaattaa tatatttgat
    54061 ttaagtgaag aattatttgt tagcccagct actattgaag gggatatagt caaagctaat
    54121 aaatttatag gaagttataa tttaaagata aagcaatcga aatttttatt gaaactagtt
    54181 ggaaatgagt ctgcaaagcg caagttaatg agctctataa tatttaaaga aacgggaagt
    54241 gattttttaa gcttatttga tgtacaaaaa atatatcaag agtataattt gactaaacta
    54301 aaagaaaata taatatatat attaaaaaaa tacaatttat ttataaatga gtatgctata
    54361 aacaacatac ttcttcattt aatgataact atagacagaa taaaaaagaa taattacata
    54421 gactctgttg aagtagtaaa ctacatagac aataacgtag atataaatat tgcaaaagac
    54481 atatcaaatt ttttagaatc tgaatacaat ataactttaa ctagtgcgga attatactat
    54541 ttagtatttc agttaacaaa caaaactaca gtcttaaact acaatcaaat ggatactaaa
    54601 tcattgtcaa attatataga tgaacacttt gttaaattaa ctaaaaaaat aattaaaaat
    54661 gtatatgact tatatcttat agatttatct gatgaggaat ttgttgtaaa atttactctt
    54721 catgtaaaaa atttaatcag tagagccaaa aataatcaag ttttaagaaa tcaaatacct
    54781 caaaagttaa aagattccta ccctttgata tatgatatat ctgtttatat ttgcaatcag
    54841 attcaaactt tagaaaatgt agatatagac gaagatgaaa tatcttatat atctcttcat
    54901 gtaggttcat tctttgatag acaaaaatta ttagaagata aagttctatg tgcattaatt
    54961 actccaaatt actatgattt acaattcaaa atagtaagag accttgagaa gagatttaat
    55021 gaatccatag aaattattca gattttttca gatactcata atcttgattt tgacaataag
    55081 gtagacatgg ttattactac tttaccaatt aacgatagat gccctatacc ttttgtttat
    55141 gtaaatcctt acctaaatag aaaggattat gataatattc aaagtaaatt tattcagatt
    55201 aaagatagaa aaaatatact tactgtacaa aatcatttag aaatgtactt tagtgaatct
    55261 ttgtttatga aaaatatata tttagatagt gctaaagatt atataagatt tatgggaaat
    55321 attctgtacg aaaataaata tgtcaaaccc aattatatag atgatgttct tataagagaa
    55381 aaaatgtctt caactgcatt taataacaat gtagcaattc ctcatagcat gaaaatggat
    55441 gctttaaaaa ccggagtatg tttaattgtt aacgataaac cagttaaatg gggtgaggaa
    55501 aaagttcaaa ttatagctat gatacctata aatgaaaaag aaaaagaaaa attcaattac
    55561 atatttgaaa gctttattga aatattatct gaatggaata atgttaaaga acttactaag
    55621 gcagataact atagtagttt tatgaataga atagcttatc ttattcaaaa tatctaaatc
    55681 cattaattta tttccaatat atgtttctta agttattaac ataaaataga taagctgtca
    55741 cactaaaaag tctaaattaa tatgtgacag ctttttacct aatatacctt gttttaatac
    55801 tacatatttt ttgactgttg tatactagtt ataagattga atatttgtgt tgttgtagct
    55861 gtcatattct cactagcagt tgctttatcc attgcttctt ctattgtcat agctctattt
    55921 actatagaaa aataagcatc tataccctct tcattacatt taactgcatc ttctgttgtg
    55981 gctccagcca aacctattac ttttacccca tatttttttg ccagttttgc aactcctatt
    56041 ggagccttgc ccatagcagt ttgattgtcc aatctacctt caccagttat gacaaaatct
    56101 gcatctttta actcttcctc taatttaatt tcttctaata ttatttttat tccagattct
    56161 aattttgaat ctaaaaatgc taaaaaagca aaacctaatc ctcctgctgc tcctgctcct
    56221 tctacattag ctacatcttt acttaagtaa tttttaacta ctttggaaaa atttctaagc
    56281 ccattatcta acttctcaat tatttctgat gttgcacctt tttgagaccc gtatatgtat
    56341 gcagctccat ttttcccaaa cagcggatta tttacatcac aggctatttt aaacttacat
    56401 tcatctaatt cctttaatct attttctatt tttatatgtc ttatttcatt taaaactttt
    56461 ccacctaatc ctactaactt gttattttta tcataaaact caaaccctaa ggcttgaagc
    56521 atacctatcc cagcatcatt agtagcactt ccacctatcc caactataaa atttctatag
    56581 cctttattta ttgcttcctt tattatttcc ccaacaccat aagttgtagt gtataaagga
    56641 tttctaagtt cagtcggaac caatgttaat cctgatgctt gagctatttc tattattgct
    56701 gtacttgtat tttttaataa tccatatgtt gcatccactt ttttagctat tggtccagta
    56761 acttctatag tctctttttt accattcatc ccttgaacta aagcatctac tgtcccttca
    56821 ccaccatcag caagtggcaa tatctttact tgcgcatctt ctttaacctt caatatcccc
    56881 ttttttattg catttcctgc ctctattgaa gataagcttc ctttcaaaga atctatagaa
    56941 attaatattt tcatacttat ctactccttt ttattactcc ttattttaaa catataattt
    57001 tataagctta atttatcttt tattttatac tctacatctc tctttgtata caacttatac
    57061 cattaatatt cttttatctt ataaaaaggg agtgtctcaa aataaatttc ttagttcatg
    57121 agacactctt ttgtataaaa tcaaatatat gtttattatt tcaacaatga taaggaggat
    57181 tatctctatt ttaagataat tcctttttag cactatctaa tcatttttag cactatttaa
    57241 ccagtatagt tttaacttgg ttcattaagc actctacatt tacagagtta ttatctattg
    57301 atatagattc taattctttt tcatttaggt taatccattt agctgtttta atatttttgt
    57361 taaatactat gttagctgat tttgtttctt ctttagaatt ataaaatcta actactaatg
    57421 cctcttcttt ttcagccttt tttacaacac ttaacacaag gtccttatta gtttccttaa
    57481 gtaaactata tgaataatct acttctattt ctgattgatt caatttcata gcattatatg
    57541 gcattttatt ataagtttgt atttttgtta aatactcttt agccatattt gcaacatttg
    57601 cttctagtgt agatttttca tgagtaacta ttgctaagtc tatttttata tctcctatca
    57661 tttgagaatc tggagtaggc aactttattc ctgatggacg acctgggcgt cttgccattt
    57721 cttctttacc taggaatcct acacttctaa ataatgttat agctattgtg tcaaattttt
    57781 cccctactat ttcaaactct cttgtactgt ttgtaagaac agctactcca tgctctttgt
    57841 cagacaatcc aacgaatgtt agcattggat atatagaatc tggtctttca ctccagtctt
    57901 cctgttgcca aacttccata gcatcatcat acacacttct ctttatatgt ccaaactgat
    57961 tatcagacac agaaaacttt gaagctatat ttgttggtat ataagttcta accctacaat
    58021 ctcttgcttg gttatttatg ttgaatacta catctattat agctttatca tttggaacat
    58081 ttataactat atcaaagtca ataaatgaat ctgctctatt attttttcta gcttgtatat
    58141 cttttggtac ttttaatcta taatttatct ttatctcatt attaaatcta tttttcttta
    58201 tagtggaatt tgctactata ttatcgttaa atattaattt ctcatttgga agtggtgaaa
    58261 aatcatactc atctccttca tctcctccat tttccatcaa taatacattt tcaaaagttt
    58321 tatttaattt tttatcaaat atctttaatg tcccgttttc attaacttga atagtataaa
    58381 agtcattatc taccatgtca catacttcat aagctttttc taacatatgt tcaacttctt
    58441 ttatcatata cgccttatac cccattgcag gtattttatc tttaaagttt attgtatata
    58501 ccataaatga atcatagttt ccataatgaa caatttgacg gtctattaat cctgcatcta
    58561 ttacttcttt atgtaccact tcaaaatcca acttattatt atctttatca aatatttcaa
    58621 atgctttcat tttagttata acttgggcgc tcactatttc attcctatcg taaggcatta
    58681 agttaaatgc tattaactta tctaaagcca tttcacaact cattgcatca actattcttc
    58741 ttttgtaaaa tgttattaat tggtctactt tttcctcagc taagaaaaat ctattcatta
    58801 tttcctggtg aactttatct gaacaacaac atccaatgct gtcatgagca tgatttttca
    58861 ttatttcttt ccaaattaac tctattaatc catgatgata ttcaaatcct agagaatatg
    58921 caatagaagc tagtggctcc aatatgttag ttatctttga ttctattcta gcatttgctg
    58981 attttatatc cattcttgtt gagaatatac ttctatgaac cctcatatat ttcccatcta
    59041 aaaattcccc ttttattgta tctatatcct catttttttc tagctcttta aatatgtttt
    59101 catatctact taagaaaaat tttctgtctg gataacattc ttttaattta tatataactt
    59161 caaatatatt cttttgaatt ggcatttggt catgtccatt tggaagtatt atatggtcag
    59221 tagttgcacc cttatcaaga accggtaaat atttatccat tcttgttctt agctcatctt
    59281 cattcattgg tagatatttt cctattgcat aacctaatgg aagtatttga accaaaacag
    59341 aagacccatc atcactcttc caattaaact ctgttttatt agttcctttt ctttcagaac
    59401 atcctctcca aaacattgaa tattttatat caaacccatt tagtatttgt ggcatttgag
    59461 cactttgacc aaatgaatct ggtaaatatc ctattttcat atattctcca aattcatcac
    59521 aatcttttat tccatatagt aaatttctaa gtatggattc tccaccaact accatttcat
    59581 cagtttgagt ataccaagga cctattatta gccttccttc ttgaactaat ttttttattc
    59641 tctccttaca ttcaggctta actgctaaat aatcttctaa tatagcagtt tgaccatcca
    59701 tcacataata tggatagtta tgattatttt ctaacatatc cattatttct tccatattat
    59761 ttacaagtaa tattctagat tcctcagttg agaagtacca ttctctatcc caatgcatat
    59821 gaggaactat gtgtacattt tttttcattg cttatcctcc aaaacttatc tgataatatt
    59881 ttctgtctaa ttaaaaatcc aataaaaata ctactgagtt tacaaaattt tttaatattt
    59941 attttatcac tataattaaa cctttgattt aaatatctaa taacgtatct tttaatgtat
    60001 ctttctttga tttttttgtt tcctagttat tattaataat attgctgata ttatagctcc
    60061 aagtactgcc gcaaagaacc atattgatgc tataataaat cctgacatac ttccctctaa
    60121 aagcgccaat gagaatatcc ccgctccagg cacttgaaga cctatattga agaatgctac
    60181 tatagaccca gttatggctg aaccagctac caatgatggt ataacacgga ttgggtcctc
    60241 tatcataaat ggtattgcac cttcagttat tccagccaat cctaatatcc aagtttgctt
    60301 tcctatttcc ttttctgatt ctgaaaataa atctctgcct aaaacagtag ctattgtaac
    60361 tccaaaagct gaaaccatct taactgatgc aaatattgca taaggtataa aatttccact
    60421 tgctattgct cctattgcaa atgtataggc tgctttattt attggtccac ctaagtcaaa
    60481 tgaaaccata gctcctatta ttgcacctag tagtattgca tttactccag tcatcccatt
    60541 taaccaattt ataagaccat tgttaagtgc tgctacaggc tttcctacta caaataacat
    60601 caataaacct actaccaaag aaccaactac tggatatacc caaaaactta cgaatccagc
    60661 taatgtgcct ttggctggta tattcttctt taaaaatttt actatataac cagcaagtaa
    60721 acctcctaac atacctccaa gaaatccact acctattaaa tcagctgcta gacctgctgc
    60781 aaatcctggt ccaagacctg gtttttcagc tagtgagtaa gacatatatg ctgataatac
    60841 tggaaccatc aatgtaccta acatacctcc agaaagcttt cttaattgcc ataaccaaga
    60901 accttcttga ttgtatagtt cttgtagacc aaatgcttga gatgctaata ctgcaaatgc
    60961 aagtatcata cctcctgcca caataagtgg tattatatga gatactcctg taagtattgc
    61021 tgattttaca tcttttttcc atgatgattt ttcacaatat gatatatcag atgatttttc
    61081 tatataatca cctttacctt gtttttgtgc tacttctaaa gcttcgtata ttatttcttt
    61141 tgctcttttt agtggagctg ctactgacgt ttttatgttt ggcaaatgtt caaatctttc
    61201 tgtattttta ggtgctacat ctgttgcata tattattgca tctgcccttt ttattatatc
    61261 tggcgtatga gggtccacca taccattagc tccttgcttt tctacataca catccacacc
    61321 tatttcttta ccagctttaa ttaatgcttc tgctgacata tatgtatgtg ctataccggc
    61381 agggcatgct gttattgcta atataatttt ttttgattca ttttgaatcc taatctcttc
    61441 ttttatttgt tctttctttt cttttatatc taagttatta aataactctt tcttagtttt
    61501 tgaatttaac aacatattca tataatcttc atttgacagt ctagtaacta actcagaaag
    61561 tatttgtatg tgtgtatttc caccttcact ttctggtact gctattaaaa atattagctc
    61621 tactttatta tttgggtcta cgctttccca attttctatt ggtgtctcta aggtcatagc
    61681 tgcaaatgca gcttctttaa ctgacttaga ttttccatga ggaattgcaa gccctctttc
    61741 aaaacctgtt ggagataaag tttctctgta cattacagct tctaaatatt cctcataaga
    61801 tgttaacttg ccttcatcat atagctttct tgctaagaac tttattgcgt cttcctttgt
    61861 ttttattttt tgttttaaaa aaacaaggtt ttcatttgtg attttcttaa attccaaaat
    61921 tacgccctcc ttaaatcatt taatgctatg aatatagcat aaaataaatt atgaaaatgt
    61981 ttttttgaaa ttatacacac aatttcttct taaaaaataa agatttgtgt atttatacac
    62041 aaatctctat cttaaccaat attgctctat tattgctctc ataatcatca taattggaac
    62101 cttatctgat atattgtacc catttacttg tttaattgtt gaatagcaat ataaacttat
    62161 atctgcaatt tgttgcaatt gattttcaca tatatgtgtt aaagctacta ccttacaacc
    62221 cttattaact gctgtctttg ctgcttctat aacttctttc gtttctccac ttaaactaag
    62281 tgcaaaaaac atatcttgtt cacctaattt tcctatatca tgaagtactt catgtctttg
    62341 actatagtaa gttatattat catgcccacc tctaatttct tttactaata tctcacataa
    62401 aggaatgttt tgacctattc cataaactat aatactccta gatttttgaa ttatatctgc
    62461 tactttattt attttttcat aatctattag ctctaatgtc ttttttatat ctgattcaaa
    62521 accatttttt ggctctttat cctcattttt taactcttct tttaaattat tttttagttg
    62581 agagaaacca tcatactcca tttttttaca tagcctaact atggtatttg ggacagtaaa
    62641 aaatttatct gctaaagatt gtattgataa ttttactatt tcttctttgt tttttctcat
    62701 atattcaatt atcatgtctt ctgtatctgt aaatttatac tcatattttt ttgctctttc
    62761 ataaaattcc atagtttctc ctccatctat tttattattc ataagtaaaa ttcaaaaata
    62821 ttagctaaaa ttttcgttaa tataattata atttaatatt tttaatttat taatgcatac
    62881 tactcttttt attatacata aaaaatgtgt acttcctata ttgatagaga tgattcaagt
    62941 ttttattttg atacatctct atcaatattt ttttagaaaa tacacatttt tttcaaatac
    63001 ttatttttaa ttaaaacttt aggtaatcta ttctttatat ataattatta gacttgaaaa
    63061 tatctttaaa atcaaccaat tcaatattat ttctttttaa ccacatttta acttccttat
    63121 cacatagcat tgcatgttca tatactctct cttgaataag agaagatgat tttataagac
    63181 tataatctat aaatcctgga tgagtaacca tcaatgttat atctttatct aaatttatat
    63241 atttttcttc aaataattta acaaaagatt tatttaactt gtaaaaatca ttctgaggat
    63301 atttacttgg catgctaata catgaatcca aataataggg acaacttggt ataccgtatt
    63361 cttttgcagc atcacacatt gctgatattg atacatcttc ttctatacca tgtatatcaa
    63421 tatactcagg caatttactc atatacaact tgtaagtttc aatttgagca caaacttctt
    63481 catatacttc ttcataatta aaaacctctt tacctgtcag tagttgcgtt cttcttattt
    63541 tgctagaaac aaattttcca ccttcatcta acaaattctt tatcttaata tgttctttgc
    63601 aacaaggttc tcctattact aaattaacat gtaaaccaaa acatacatca tcatttttta
    63661 ccatttctat agcttgtctt gaaaaaggca tattcaccat gattccagca cttgtaacaa
    63721 taccgttctt ataagaagat attatcccta gtgtaacacc ttctgaatat cctatatcat
    63781 ctgccctaac tactaatttc ttcattttat gcatctgcct gctttctcaa ctcatcaagt
    63841 tgcatttcca tctcatcctt tactttaaca acatccgttc ctatgatgat ttgaactgat
    63901 gtattactca atttaattac ccctttggct cctgtttttt taatattatc atcattcaaa
    63961 atagtggcat ccttcacttc taaacgtaat ctcgtcatac aattattaag agtcactata
    64021 ttttcataac caccacaatt tttaattatc tgtttagcca tatatgaaaa attaccatga
    64081 gaaagtttta aattcttttc tttttcacta actttatcac ctatctctat ctcaacttca
    64141 cgtcctggtg tttgcacatt atcttttatt attatatatt taaatgttac ataatataat
    64201 acaaagaata caactcctac aggtataact aaccacgctt tatctccata actaaaactt
    64261 aagacatagt caattatact tgctccatta aatgaaccca agcgagcacc caataaatat
    64321 agtacaactc cacctagacc agaataaaaa gaatgaatta tatataactt tggagatgca
    64381 aataaaaacg aaaattctat tggttcagtt actgttgcca taaaagaagt caatgctcca
    64441 gcggtcattg caccttttac ttcttgcttc tttttatctt gtgctgtttt atatatagca
    64501 taagaaatag caggtattga aaacatcata attatgtaaa atcctgtcca gaagaatcct
    64561 gcatttgggt ctcctgccat aaatctagga atttctcctc ttataacttc tcctgttgat
    64621 gttgtatatt cacccatttc aaataatatg taagcattta aaacttgatg taacccagtt
    64681 ggtattagtg cacggttaca gaataaaaac aagaatattc ctattgcacc agctcctccc
    64741 aaccaaaaac caaattgatt cattgcagct tgtggatata tccataataa actaaatata
    64801 tatgataaaa ttaaagcaac cactggtgat agtgtaatta cacatttttc tcctgcaaag
    64861 aagcttaaaa atgctggaac tttaacttta tatgttttat tgtaagtcca tgctatacaa
    64921 gctccaacaa ttatcccacc aaatacacct gcatttaatg tatcattcaa taactctaaa
    64981 tttgacttaa atatcatata tgcaactacg gctgcaacca ttacacttgt cttgtcttta
    65041 gactttgagt aggcactagc tgctcctaat aaaaataagt agtccacatt tgaaaatact
    65101 atattagata tattagatat agctggcaca ttgagcatat ttggaccaaa taataatcct
    65161 aatatacctg ctgctggcat tacagctatt ggcaccagca tagtctttcc taaaagtgac
    65221 agttgttgca tcattttgtt taagaatctc ttcatatcca atcccctttc tatttcggtt
    65281 taattttttg tgaaaacgat ttattcacat tttatatttt aaatattata tttgtttaaa
    65341 caaattgtca atatttttta ttcattttat ttttatcatt tagtttaaca tataaatcct
    65401 tttaagtgta ttgctatatc tagtctattt ttttaattca ttttttttat tttaaaaata
    65461 tgtttaacca tgtatttgaa acaacccaca aaataattct attttgtcct ttttatggtt
    65521 gatatgattc tattttcact atacatgtct aaacacattt gatataattt gttcatatca
    65581 gttcaataat attactaaag acctttaagg agggataggg gtatatggct aaaaataaat
    65641 tttcagagat atatgaagta ttgaaagagg aaatacttga tggtaaatat acaagtaata
    65701 tgatgctgcc tactgaactt caacttattg aaaggttttc atgtagcaga aacactgtac
    65761 gtagagcaat ttctcaatta aacacagaag gatatgttca aagtataaaa ggcaagggtg
    65821 ttgtagtgtt agaaaatagt tgttctaatg atttttttct aaatatgcac aatttcaaag
    65881 gtgtagaatc gattgtagaa gataaaaagg ttaatactgc tactagtgtt ctccacttca
    65941 gcaaaatctt aattgataat aagttatcta aaaagacagg ttttaaagtt ggtagtgaag
    66001 tatattacct tcacagacta agatatattg ataacatacc taaaattctc gacattaact
    66061 attttctatg tagtatagtt aaagaccttg atgtttctat agcacaaggc tctatttata
    66121 aatatataga agagtgtcta ggtacaaaaa ttgtttcatc aagaaagata tttaaaattg
    66181 aaaaagctac agaacttgag ttaaaaacgc tacctctaaa tgattataat tgtgttggtg
    66241 ttattaaaaa tagtgtatat actgatgatg gaaagctttt tgaatatact gaatcaaagc
    66301 atacacctga aacatttgta ttcatggatg ttactcaaag atattaaaaa aattatgttt
    66361 aattaaacat aattttttta cgtttagaca ctttatccca tactaacctc actattctat
    66421 atacaattat atgtttatac aaataattat ttattgtata ttttagcttt aatattagtt
    66481 taacgcttga caatttgttt aaacatatta taattaactt ataaattaca ggaggtatca
    66541 agtatgaaaa attggtggaa aaaagcaact gtctatcaaa tatatccaaa aagtttcaaa
    66601 gatagcaaca atgatggtat aggtgatata aatggtatta tagaaaaatt agattatctg
    66661 tattctttag gtgttgattt attatggttg actcctatgt acgtttctcc tcaaagagat
    66721 aatggatatg atatagaaga ttattataat atagacccaa aatatgggac aatgagtgac
    66781 tttgaaaaac tcttaaaaga agctcataaa agagacataa aaataatgat ggatatggtc
    66841 ctaaaccata catctactga acataaatgg tttaaagaat ctaaaaaaag caaagacaat
    66901 ccatatcgtg attattattt ctggaaagat gctaaacctg atggttctgt tcctaataac
    66961 tggatatcca gatttagtgg aactgcttgg aaatatgatg aaaccactaa ccaatattat
    67021 ttacatttat ttgaagaaac tcaagctgat ttaaattggg aaaatgaaaa ggttcgtgaa
    67081 gaatgttaca aaatactaga attttgggct gataaaggta ttgatggttt tcgtttagat
    67141 gtagtcaacc tactctccaa aacacctgga ttacctgatg accctataac aggaccaaaa
    67201 ggagatggaa gaactcacta cgctgatggt cctcgtatac acgaatattt acataatatg
    67261 aatcaaaaag ttttcaaacc taaaaatata gttactgtag gtgaaatgtc atctactaca
    67321 cctgaagagt gtatcaacta tactagagaa aatcgagaag aactatctat ggtttttagc
    67381 ttccatcata tgaaaacaga ttataaagga ggcgcaaaat ggactaatga aatgttctct
    67441 ctggataaat taaaaaaagc acaatctgac tttcaatata aaatgtatga aggaaaagga
    67501 tggaatgctc tattttactc taatcacgac caacctagag ctttatctag atttggagat
    67561 gatagatttt ttcgtcaaga aagtgctaag atgttagcca taacattatt tggtctacag
    67621 ggtactacat acatatatca aggtgaagaa ataggtatga caaatgccta ctttactaaa
    67681 atagatgaat atgatgatgc tgaaagtaag aatgcatact atcatatgat taaatctggt
    67741 attgatgaaa aggaggcatt gcttatactt caacaaaaat ccagagataa tgcccgtact
    67801 cctatgcaat gggataatac tatttataag gggttttcta atcataaacc gtggttaaaa
    67861 gttaataatg acaatattag tgtagaaaat gagcttaatg attctcgttc agtattttat
    67921 acttatcaaa gattaatcaa atatcgtaaa caatatgata tttttacaga aggtacttat
    67981 agattgttag atgaaaatca taaaaatcta tttgtttatg aaagagagta taaaaaccaa
    68041 aatcttcttg ttataagtaa ttttactcat gataatgtag tatataaatt acctgaaact
    68101 tatttgaata aattaaacta cacaatactt gaaaccaatt acactagaga tataattgaa
    68161 aataaaatgg aaataaaacc ttacgaaagt ataatgatat attataaata aagttattta
    68221 tgtaaaaatc ggtataacac cctggtgtta taccaatttt actaaattca aagtaatatt
    68281 tatagaatat ctatagatta caataattat ttgacttcta catcattata tttctttgaa
    68341 tatactttag tcatatagaa atatatagga actacaatta tcatcccacc taatccccaa
    68401 actatcttca taggttcatc aatacctgct tttatcagta ataatatact tataataact
    68461 gcaaatattg gtataattgc tcctccaggt attctaaaat ttacatcttt accagcatac
    68521 ttctttctca atattagtac agctaaacat gtaggaatat attgaataaa tctagataca
    68581 acacttattt gagataatgt tgtgaatgaa cccgaccatg ctattagtaa agttcctatg
    68641 acagatacta taattgctat ataaggagct ccatttttat tagtttttct tatagactta
    68701 ggcattaatc cttgttcaac caaagcagca gcagaccttg gtgtaaatat ggatgatgca
    68761 atatttatac ctccaacaga tactatagtt cctattgtaa ttatataccc acctatagga
    68821 cctaacatct taatggcagc atctgcaaca ggagtagctg accctgctaa gccattaccc
    68881 aacaccccta tacttattcc cagtataagc atatatatta ctgatactac aaacattacc
    68941 attaccaatg ctttaggtac atttttctta gggttctcca tatcttttgc tgcaacagct
    69001 aaagattcaa atcctgtaaa agcataaaat aaagttatag atactgctac aaaagctgat
    69061 gacgcacttt gacctttttg tattattaca aatggggtaa aattagaacc ctttataaag
    69121 aataatccta cagctataaa taaaactatt ggtaataatt ttcctatagt catgatatta
    69181 tttaagtgct tagattgttt catccccata aaatttataa tcccaagtcc tacaactaat
    69241 ataccaatta ttatattttt agtaagtggt tctgctgctt gtggccatac agatgatagt
    69301 gttacagcaa agaaatttgc catagtagcc caagcaatca tactaactat ccacttcata
    69361 aatccaactt caaatccaac aaaatctcca aatgcctctt ttgcataaag atagggccct
    69421 ccatcagtat caaaccaact acctgcttca gcaaaacata aagcaataga aacagctaaa
    69481 agcatacaaa aaactaatac taatatactt gctggaccta tatctgtata agcatcacca
    69541 ggtaacccga atatacctga ccctattatt gcattgattc ctaatagtac aatactccaa
    69601 aaacctaact taatgccttt ttccattgtt attcccccca aagccaactt aatatatttg
    69661 tattttttat ttagatgctt ctttaactat tgccataaat atttttttca tattatcata
    69721 atccctagtc atcatttctg gatgccattg tatccctatt acaaactcac ttccttcctt
    69781 ttctatagct tcaattaaac cgtctttaga tgtagctgaa acaatatatc ccggtgctac
    69841 tttgttgaca actaagtgat gaaaactatt aactaaaact tctttttctc ctaatatttc
    69901 atataatttt gtcccctcct ttatctgtac tgtatgtgta ggtacattag atagatgttg
    69961 ctgattgtgt ttaatataag caccttcaat taaagataaa tcttgataca aactacctcc
    70021 ttcagttaca tttataattt gttctcctct acatatacct aaaactggtt ttttcatatc
    70081 taaagcatgt tttaataatt tcatatcaaa actatctctc tttggtgata ttgcccccaa
    70141 cttattatga ggttcttctt tccatatgag tggatttaca tcttgtcccc cagatagtat
    70201 aagcgcatct atattagata cctgctcctt tataatctca tcatcatata caatcggtac
    70261 aatatatggt atagctttac acataactac tgattgtata taatcattat ttacataagc
    70321 cctttcatag ccaggaaaca ttcctccttc atcaattagt aaattacctg atattcctat
    70381 tattggatat ttctttttag ccaatttctt caccccttaa cttatttgtg aacaataaaa
    70441 aatgctactt ctcttaaaga gcaagtagca taccaagatt ttatttattt gaacattata
    70501 atttatttta gaaatacttt ttttgaatac gagtaatata aataacttta tttacttata
    70561 ttttatttta atcctgttta gtttgacttt tttaaattgt tttaagtaca actaataggt
    70621 aaaataggca tttagatttt cctttttatc aacctattaa atactgttta atatattttt
    70681 tccccttcta tttaattcac ttccctttct accagattca catattatca aatccagctc
    70741 ttttaacata ttaagtattc cccttacctc accttctgtt atattgtagc ctaattcatt
    70801 tattgaatta acaataccat ttctacctat atttttatta agattattgg cattttttaa
    70861 acactccatt actaacttta ctttctctat agatgttcta tcctttaata tacttatttc
    70921 tttactaaaa tcttcagtta tatttaataa gttatgaggt aaatcattta ttaaaataac
    70981 atcctcagaa ctcataatgt taatatacat agcagtattt tttaattctc ttatattacc
    71041 tggccattca tatgcattaa taatgtcttg aacatcttga ctaattgctc tcttcctttt
    71101 cataaaatgc tttaacataa cttctatatc ttcttttcgc tctcttaaag gtggtatatt
    71161 tatgggcaat acatttatcc tatagtataa atctgctcta aatctatctc cttttatcat
    71221 ttctaataaa tttttatttg tagccgcaat tactctaaca tctatatcta ttacattttc
    71281 tcctccaacc ctcattactt gattctcttg taatactcta agtaatttgc tttgtaagta
    71341 tataggcatg tctcctattt catccaaaaa tatagttcca ttgttagcct gttcaaataa
    71401 tcctttttta cctcctttta aagctcctgt aaaagcacct tcttcatatc caaaaagttc
    71461 actttcaagt aaattttctg gcatagctgc acagtttatc gctataaatg gctgactatg
    71521 tcttggtgat gcattatgaa tagactgagc catgagttct tttcccgttc cactttctcc
    71581 cgttataaga attgtataat cagattcagc tattttcata cctaatttct tagttctttc
    71641 catcttcgtt gaaactgttt ttatatcatc aaaagtatat ctagcaactt gtcccttttc
    71701 tctaagcttc tttgataggt tttgctccaa ttttcttatg taagttattt cttgaataca
    71761 aaaataatat ccagtaaccc ttccaaaact ctctatatta tacttattta cgtttaagta
    71821 cttattatta aattttataa cttcatcatg tgccttactc aaactaaaaa tctttttaat
    71881 attctcattc tctaattcat ctatattttt ccctataata ttactttgaa tatctagtat
    71941 atcccctaaa ctattattgc aaataattat gtttccctta tctgatacca ttgccatacc
    72001 taagtttgat aaatttatta tagaatttaa ttcatctgct tttatagtaa gttctttata
    72061 tttagtattt attcctgaat ataaactaat tatttcatct gtatacttaa ttagcctttc
    72121 agtaatttct ttatcatcca gtttgagttt tgctattatt tgaataaacg ttgaactatc
    72181 aatataccta tggcctaaat caataacttc ttctatatat tttggaacta aacttgcctc
    72241 tcctggcgtt atagctatct taatatttct atattcttca ttgggattgt atggtataaa
    72301 attcagatga tttattccta tattgtataa attagatatt gtttccaata cagtatgctt
    72361 attatcgttt actactaata catctattcc ttctggcaat gaaaataact tatatatatc
    72421 cttttgcttt atgctcctat ttatagttat tatctttgag acatcttcta catatttttt
    72481 tatttttaat actctatcat ctatcattac caatattatg tcatctttta ttaattcctt
    72541 ctctgttatt ttatttaaat aataattttt tatcattact ttatctttaa atatcatttc
    72601 tagattttta tttaagaaac attctaattt agacaaccca tcagttacta ctgctattgt
    72661 tttcatttga ttactccttt taaaatatac tctactcttt tataatatac atctgatttt
    72721 cactttaaca gcacaaaaaa ttgtacattt agtatactaa catattcctt tattagtttt
    72781 atgtccctaa atatcatagt tgcaatatct atcatatttc ttaaacttct ataaagcaaa
    72841 aattgtgcca aattaataat tttcttaatt ttattctaaa gttcctcaat agtatttaca
    72901 gacttagcac ttatagctta taagtcttta tattattaat ctatttttat aggttaattg
    72961 tacaaatcat atatcacttt tacccattaa atgcattata aaaaagatgg aataacgatt
    73021 cgtcactcca ccttttttgt aataaaaaat tgtatatata aacactattt actactcttc
    73081 taagtacaaa cattatctac atactataac ctttcaccat tagattctat tacttgttta
    73141 taccagtcaa aactcttttt cttaatccta gacaagtctc cacttccatc atcatactta
    73201 tccacgtata taaacccata tctcttggcc atctcaccag tagatgcact tactaaatct
    73261 atacatcccc acggagtata tccaaataaa tcaacaccat cttctacagc ttccttcatt
    73321 tgctctatat gtgcttttaa atactctata cgatagtcat catttatcac tccattagat
    73381 tcttttttat cataagctcc taaaccattt tcaactacca tcataggtat tctatatcta
    73441 tcataaattt catttagtgc atatctaagc ccttcagggt ctatttgcca tccccattca
    73501 ctagacttaa gatatggatt tttcactcca cctaatatgt tgccacttgt attatcttct
    73561 ttagaatcac tacttacaca tgtagacata taataactaa atgtataaaa atctacacaa
    73621 ccttctttta aaatttctaa atctcctttt tccattttta tttcaatatt attttctcca
    73681 aaatatctat tcatataagt tggatattcg ccacgcactt gtacatctcc acagaaataa
    73741 ttcattactc tactatctct ttgagtttta actgcatcat ctggattaca agtataagga
    73801 tacattgttg caaatatttg catacatcca acttgataat ttgaatctat ttcatgagct
    73861 aacttaacag ccttagcact tgctacaaat tgatgatgta acccttgaaa acgaagttga
    73921 ggtatatcta cttggtttat aaaatctgta gtcccttcat ttaatattcc ttgtgataag
    73981 tagcctccca taggcattgt agctgaattt atttcattaa atgtcagcca atattttact
    74041 tttcctttat atcttttaaa tattgtttca cagtaattca tatacaagtc tattacatct
    74101 cttgaaaccc atccattgta tttctctgtt attgcaaatg gtatttcata gtgagatatt
    74161 gttattaatg gttctatatt atattttaaa cattcatcta tcacattttc ataaaattta
    74221 agtcctgctt catttggttc tttctccatt ccagttggaa atattctagt ccatgctatt
    74281 gaaaatctaa ataccttaaa tcccatttct gcaaataaag ctatatcttc tttgtatctg
    74341 tgataaaaat ctattgcttc atgacttgga taaaatgttc ctttttctat aactctcgtt
    74401 attcttttac ttactttttg agaacctcca gtacacatat ctgaacaaga aggacctttc
    74461 ccatctaaat tccatgctcc ttcacattga tttgctgctg ttgctccacc ccataaaaaa
    74521 tcttttctaa gtgccataat tctacctcca ataatttatt tatagtaact ttattttaaa
    74581 ataaaaatac atatatataa ttcttaatta atagaactct cttttacgct aaaacagtta
    74641 tcaattcctg actattattt acatgctttt gtgtattttc taaaatatcc acataatcag
    74701 aataattagt aacaactact ggtgtttcta agcaatatcc ttcttttgaa atactttcta
    74761 tgtcaaaagt tattaatatc tgaccttttt taatcttatc cccttgatta acaaaagatt
    74821 tgaaatattt accttccaac tgaatagtat ttaaccctat atgtatcaat agttcacaac
    74881 cattatcaga tataattcct attgcatgtt ttgttggaaa taaagtcatt acagtcccat
    74941 caaatggtgc cactactttt ccttctttag gtgttattgc tacacctttt cccaatatac
    75001 ctgttgaaaa cgctgcatct tgtatctgct ccaaagcctt tacttcacct tttattgggc
    75061 tatatactac ttctcttttg tttttttcta taacgccttg cccattatca tctttagaaa
    75121 tttctactat agcatcatct ttccaaaaaa ataatgttaa aacaaatcct actacaatag
    75181 ctactattat tgctattata gttgcataaa cacctttcat atctccagtt gttggattta
    75241 tataattagg tataccaaat attccaagtc cacccattac ataacttttt acattcatta
    75301 tccccattac agctcctgcc acaccaccac ctacacagct atatatgaat ggtgtttttc
    75361 ttggtaaagt tattccatat attgcaggct ctgttacacc acatatccct gaaataactg
    75421 caggtataca cagctctttt atcttcttat cttttaattt aaagtacatt gctgctacta
    75481 ctgctgtttg tgcaaaagat ggtacaaatg ctcccactaa tattgtgtca aaccccatag
    75541 tcataaggtt tgacatagct attggtacta atccccaatg tagaccaaat attacaagca
    75601 cttgccacaa taaaccagtt aatgcaccac ataaaattgg tgaaaagtta aataaagcta
    75661 aaaatccatt tccaactata ttagaagcat aggtagctat aggccctata accataaatc
    75721 ccaatggtaa agaaactaat aaaactgcca ttggtacaaa gaaattcttt actacatctg
    75781 gtattatttt agcaaacact ttctccaact tagctgctaa taatactatt aaaattactg
    75841 gtactactgt tgaagtgtag ttcattgcta ttactggtaa tcctaagaaa gttatatata
    75901 ctggtgattc aaatattgtt ccagtaaata gattgtataa aggttgacct gtagatgcta
    75961 gagtatctaa ttgaatacct gaatagcata aaactgcacc tataagcatt ccaataaatg
    76021 gttttaattt gaatttctta gcagctgtat atccaagaat tattggcata tactggaaca
    76081 ttgcatctcc aattgcattt aacataatat atgcaccatc agtttctcca tacaaaccca
    76141 atgctgtaaa tactgcatta aatcctttta tcataccagc agcacacaga actcctaata
    76201 ttggttgaaa aactcctgat attatgtcta taaacttatt aagtaatcct tttggcgcat
    76261 ctgagtctga attatctatt gtatctcctc ctaatccagc caaatcgcac acaacttcat
    76321 aaacatctgg aacatgatta ccaataacta cttggtattg acctgcactt ttcattacag
    76381 taactatacc atccattttt tttagcacat catcatttgc tattgactca tcttttaatt
    76441 taaaacgtag cctcgttata cagtgagata aactgctaat attatccttt cctcctacat
    76501 tcgcaatgat ttcttttgat agttgttcat actttctcat tttttctcct ccattttctt
    76561 tttgaaatat gaaggtttgg ccaacttaat gtcaccaccc aatatatata aacctaatta
    76621 ttaggattta tatctgactt aatactttta cttaccactc tttcaacgtg aatagttaag
    76681 tataattttt cctcatctgt taaatcgtag ttgtattttt tatgtataaa ttcatccaac
    76741 ttttctatga atttataagc atttttatat ttaactttta ttacatttaa taaatcatct
    76801 tcattatttt catgtaaatt attactaaca attcgttctg caaaatactt taagtgagta
    76861 ataaatctat gataataaac tgaatcttca tcaaatacta ttctgtaatg gtatttaacc
    76921 atatttgaaa tttcttgtat tatttgtgta atctcatata tagtttttat actttgatcc
    76981 atttgtgcat taactatatg cagtgctata aatccagctt catcttctga tagtctaatt
    77041 tcaaattttt tttctatata atccaatgcc tctaacccta ttttaaattc actcttataa
    77101 aatcttttta tatcccaaag taatacattt tttacactta ctccttcacg agctctttct
    77161 attgcagtat acatatggtc tgttaaagaa atataaatac tatcatttaa tcttttccct
    77221 agtttttctt tagcatataa aattatttca tctgataatt ccatatatct tataggaata
    77281 tctgctatta gttctttaaa tttatcagat atatttgggt ctgaaagttt gaatatctta
    77341 tcaattttat cctttgatat atgctctcct gtacgttttt tataagctaa acctctcccc
    77401 ataactacta tttccttatg attttcatca gttgtaataa ttacattatt attgagtatt
    77461 ttttcaataa tcattgtgat gcccccttta ttagcattaa gttaaaataa atctttttta
    77521 attatttaga atatttaaaa tgtaatttat tacataaaaa aaaacctatt tattacacat
    77581 taaatagact cccatgagtc ctgagtaata aataggttta gcttgtattt aaacaatcac
    77641 tatccgtttg tattttattt taattattat ttattatatg ttagcaaatt tattttttag
    77701 tgtcaacttt ttctcaatat tttaatttta aataaattat ataaattgaa tcttcaaagt
    77761 actattttta tcaataaaca aaataaaaaa ggtattactt tctttaagta atacccattt
    77821 atttttaatt taagtggttt ttcttaatct catcaagtag tccatcttct aaataaattt
    77881 ctaatgcaca tcctaccatt gcctttactg ctttgtgaag tttatcataa gcttcctcag
    77941 aatttgcata atttagaaat tctttttcat gaacatatac cattggactt atatctaagg
    78001 ctatttctgt atacatagta gggcaaactt gacttacatt acctatatct gtagaaccta
    78061 ctggaccatc tttaccttct aatatatctg ttatacctgc ctcaatcaaa ttattctttg
    78121 ttatcttttg aagtactttg ttatttatta tgttgtcaaa agaattttca aagtatctat
    78181 aagaaagctt agctcctgtc attaactctg caccttttgc acagtttata acttttttag
    78241 ttacttcatc taaataactt ctttcagcag ctcttacgaa aaacttacaa gctgcttttt
    78301 ctggaactat atttggagcc tctcctccat ttgaaactat accatgtatt ctaacatctg
    78361 attttacatg ttgtcttagt gcatttatcc cagcaaaagt aagttgtact gcatctagtg
    78421 cattgactcc catatgaggg tgactagctg catgtgctga taccccttca aaactaaact
    78481 ctaatgaatc tatcgccaaa gccttacagt ttatattatt aaaagcttct atatgcattt
    78541 gataacttac atcaacatca tcaaaagctc ctgccttaac taaatcacat tttcctcctg
    78601 ttgtttcttc agctggtgtt cctattagta caatttttcc tttaaaattt tcttttaact
    78661 tacttaatac tatacaagct cctagtgtag atgctgcaat ccagttatgt ccacaagcat
    78721 gagctggttc tttattttct ccatatccag gaagtgcatc atactcagct aaaaatgcta
    78781 tggtaggtcc atcattatct cctaattcag ctctaaaagc agtctccata ttacaatatg
    78841 gataaactgt atcaaatcca tattccttca ttttttctac taagtatttt gatgatatgt
    78901 attcttcttc ccctaattca ggattgttaa atataaaatt acaaacttct tcaatttctt
    78961 ttccaagttc attctctatc tcataagctc tcttctttat actctctatc ataatttacc
    79021 tcctactaga atcctagata taatcctatt gtcaaaaatg caattacaag aattgttaat
    79081 actaccatta aaggaattac gaatctaacc cactttgacc aaggaatatt ggctactgct
    79141 agtgctccca ttaaaacacc tgatgtaggt gttatcaagt tagttacacc tataccaaat
    79201 tgataagctg taactatcat ttgtcttgat acatctacta agtcgccaag tggtgcaagt
    79261 acaggtattg taagtgatgc aagccctgat gctgatggta ttaaaaatgc tattaatcct
    79321 tctattaaga aagttaaatt tataaatata gtttttggta atccatttaa taaatttgct
    79381 gcagaattta aaattgtatc tattataaat ccattttctg ctattattac gattccacgt
    79441 gcaaatgcta tacacaatcc tgctgtaatt aaatctcctg ctccacttat aaatgaatta
    79501 actatctcat cttgtttaag ccctgatgct attcctgata ttatacctat agctgtaaat
    79561 atcattgcaa tttcagttat gtaccattct agtcttaaaa ctccatatat cataatcgcc
    79621 atacctattg tgaaaatggc tattaccatg gcttctcttt ttgtaaattc ctttatattt
    79681 gtatcactat taaccattaa ttcttgattt agtgatatat catgtactaa agatttttta
    79741 gggtcttttt taactttatt ggcatacatc ataacaaatc ctatagatat agccatatat
    79801 attataaaca taacaactct aaactctatt ccagaacctg gtactatttg tgataatgct
    79861 tgtgctattc ctacagagaa aggattcgta gtagctgctc caaatccagc tgttgctcct
    79921 ataaatactg tggccactgc tgtcaatgaa tcatatccca tactggtcat aagtggaata
    79981 aatatcatat aaaaaggtag tgtctcttca ctcattccaa acgtcgtccc acctagtcca
    80041 aatagaatca ttgaaatcgg tattattaaa aattgaaact tcttcatttt atttacagta
    80101 tgagctataa cagcttctat agctcctgtt ttattaacta tcccaaaagc tcctccaact
    80161 attaaaacaa atccaactac ttcagctgca tctataaacc cgtttatagg tgctgtaaat
    80221 acatcagtaa ttccttgtgg atttgatggt gctttttcat aagttccagc tacaacaact
    80281 gaccttccgt ctatatctac tctttcaaaa tttccacttg gcactatcca agtaagaatt
    80341 gccattaaca ctattagcaa aaatattatt gtaagtgtct gtggcatagt aaactttttc
    80401 ttttttaaac tgctcatttt taatctcccc actttttaaa ttaaatattt tattattgca
    80461 tccctttatc ctattttgta tagatgtaaa aaagctcaac atggatacat gttgagctaa
    80521 atgtgctaaa tacaacatac ctgcatcttc cctttatctt gtttaagata gctctccatc
    80581 aaaattcggg ataattttga tgacagtact gtacctatta aatacagtcc cagcactagc
    80641 ttcggacact agcacttcgg cacttactcc ttttatattg cctcattggt ttccaacctc
    80701 ttcaatatta atcttagtaa atgcaacctc tacctcaatc tgtttgttag atgaggtaat
    80761 tcttattaat ttttattaat tcaaagtaat tgtagcataa tatgtcgaga ttgtaaatat
    80821 tttcagtata ataattctta ctttatgatt tcattccatt tttcacaaat aattttttct
    80881 ccatataatt taaatactgt agtatatttt aaatacacac aaactttcca caaattttaa
    80941 attattataa tacctttata ataaaaaata aaagctatgt aaacttatca atttacacag
    81001 ctttatatac attaatctac tccaagtaca gctttaagct ttgatgctat tgtaataact
    81061 cgtggaccaa atataacttg aactgagttt ttctttttaa caactccact tgcacccaat
    81121 tcatttaccc agaagccatc ttcatagaat ccatcaattg aatttagctc taccctaagt
    81181 ctagttgcac agtttgtaac attttttata ttattacttc ctcctaaacc ttctattata
    81241 gaatgtacaa gctttgtatc tttatcatct acttcctctt ctactgcatt gttgtatgct
    81301 gttttaccag catctacaga ttgtctttct ctatattctt tcttactata aagcttaact
    81361 tcttctgaac ttcttcctgg tgtttttata tcaaacttct tgattgccca tgtaaatatt
    81421 atataagtaa ctataaaaaa tattggtatc aaaaataata atggtaacgc atccactttt
    81481 tgtggttgta ataaattagg aaacatattt ctaaatgatt ctcccattat agaaacatta
    81541 aacatttctg taaatacata cgtcaatcct gccagtggta catatgctac aaaatacaat
    81601 aatggtgcaa caaataggaa tgtatactct attggttccg ttacccctac caacatggct
    81661 gttactattg ctggtatcaa tatacttatt gcttttttct tattagcatt atcagccaac
    81721 ttatacatag ctaaagctgc tgctggtaat attgcaaaat gtattaataa acgacctgtt
    81781 gtgaagttac gagttatata agatagtgta tctgggtctc ccatttgagc taatcttata
    81841 ttattaacac cctcgtaaat tgctccacct acttccattg ttccacctac tcttgtatat
    81901 tctataggga atgcaattaa gtgatgtata ccaaatggta ataatgcttt atccattgct
    81961 ccaaaagtaa atgttcctaa taatccacta gaacctatcc aattaccaag tttagataat
    82021 gccaacccta ctggtggcca tataaagtac attgctgtac ccaatactat tgatgctata
    82081 aacatcataa tcattgaaaa tcttggacct gagaaaaaag ataatgcact aggaagttct
    82141 atgtcaacaa atttgttatg tactgcacta gctacaaatc cagctattat agctccaaat
    82201 atactacaat catatgaaaa gattcctaag aaagaaccat ataaagaact tcgactatat
    82261 gcttctgcct ctcctaatcc agatgccata aacgcttctg ctgtaactgt atctgaattt
    82321 ataccatttg ctgctaacaa tgtacttata actgtgtgca ttcctataaa taaaacaatt
    82381 cctcctaatg caccccatcc tttttccttt ttagataatc caaaggctat acctgtaaca
    82441 aagaatattg gtaagtatct cattaccata aatcctaaat cgtttattac tttaaagaat
    82501 acatttataa atgacccttc ttttattagt ccgctaagcc ctaatgcaga aacgttttcc
    82561 atatttgcaa aagctgaccc taatcctata aatactcctg ctacaacaag taatataaca
    82621 ggtatcatca ttgctccaga aagtacttgt aatttatcct tcatagtatt atttcctcca
    82681 tttttattga aatgccaggc tatataatgc atatatatat acttctattg ctttgtataa
    82741 tgtatctata cttatatttt catttgcctg atgtgaattt ggattatccc cctctattgt
    82801 gggtccaaat gcaactccat tttttaacac tcttgcataa gatgcagctc cttttgtaaa
    82861 aagttcggct tcttttccaa aaccttgttt atatgcattt gataatttat ttattaattc
    82921 actatctggt tttacataca gtaattttaa atccttatat atatcaacta ttaaattttc
    82981 tttttttcca atttcattaa ttctattagt tataaactcc caacttatac ccttaggata
    83041 tctaaagtct attttcatat tcaattcatt tttctctaag aagatactca ttatacatat
    83101 tgtagaaact cccatatcta catcttcttt atacatccct aatttttttc catgattatc
    83161 atctgtaaag tatgaattta aaatatccct tattataacg cctttatcat ttagtttttt
    83221 aattttctct aaatcttttg ctaaactaaa tgcacagtta tatgacctgt gtggatttct
    83281 cgataaagcc ctttctcctg tatatcttac taatacttta ccttcatcta attcttctat
    83341 ctctgtataa taagctaatg attctactaa atctttctta tcattagttt taaaaacagc
    83401 ctcaattttg tcacatacaa atccatcttc tttgtttgac tttatatcaa ctaaattgtg
    83461 atttctaaat ttatctttat ctattttctt tcttagattg aagtataaaa tacctttttc
    83521 tccatttact attgggaagt ttccatctgg agaaaatgca aattttggct gttcattgac
    83581 actaaagtaa tgctccatac attcccaagt cgtctcttca gctccaccca ctatcaatct
    83641 aatctttctc ttcggctttt cattaagttc tcttaagaaa agtaatgcat ataaaatccc
    83701 tatcaaaggt cctttgttgt cattaactcc tctaccataa agatagttat ctttttgaca
    83761 tactttaaaa ggtttactct tccaaagttc tttattatat attggcacca catctatatg
    83821 attaagcacc cctactactt cttctccttc tccatattct atatgaattg catatccatc
    83881 aaagtctttt acaacaaaat ctttgtcttt tgctatttga ataagcttat cgaaggcatt
    83941 tcttatttct atcccaaatg gttgatttat atctgttgta ctttcatctc taattgaagg
    84001 tatttcaact aaacttctaa tatcatttat gatattttct tgattggatt ttaaataatc
    84061 aataattagt tctttgttta ctatcatctt aattatttat caccccttta tattaatttt
    84121 acaaagcatt tcagttcata tctacattat aaattttgaa tattattttt tcaatatttc
    84181 aaatacttct ctaagttacc ataatgatac ggtaaacaat caacagatta tgacaaaatc
    84241 atactatacc tattactaat attttaactt atctattacg tcttttttaa tgtaagttta
    84301 ttccaaaaat acataaaaaa agatgtagcc ttagctacat cttcttatat ctattcaact
    84361 tccattatta tgctattaat atctacattt ccaggttcta tcctatcttt tactgttctc
    84421 ttaacagaat cagcatcatc cattataact acaggagtta ttatatttaa atcatttgac
    84481 gttataaaat ctaaatctac ttccattatt aaatctttag cttttacctt atctcctgcc
    84541 tttaccttaa catcaaacgg ctttccttct agtgacattg tctctaaacc tacatgtatt
    84601 aatatatttg tattatcatt actcttaatc attattgcat gctttgtatc aaatacattg
    84661 actatttcac catcaacagg agaatataac ttaccttcac ttggtattat tgctactcca
    84721 tctcccatca tcttttgggc aaaaactgca tctggaacat cttctaagtt cactaaattt
    84781 cccttaaaag ggctatatat tttctcaatc ttattttctt ttttcttaaa tatattaaac
    84841 ataacacact ctccacatta taaaatttta tgataatatt tttcttaaag cttttgctac
    84901 accatcattt acattagtat cagttacaaa atctgccata gattttactt catctgtagc
    84961 atttcccata gcaataccaa gacctgcaaa tttaatcatg gatatatcat tttcattatc
    85021 acctatacaa ataacttctt tgctatctat acctaatata tcacaaaatt ttttaactgc
    85081 aatacctttt gaagtacctt tactcataac ttcaaagttt atctttccag aacttactac
    85141 ttctaaatct tcttgttttt taaattcttc ctttgcttct ctcaaagaat ctacgttttc
    85201 tgagaaaagt atacatttta aaatttcatt atcagctttt tctaataaag tttcacaact
    85261 tgcattttct attatttcaa ttctgtcttc ttctttattt ttactattta ttaatctata
    85321 tggatgttct tcacctattt cattgttgga tataactatt ttacagcctt taaaatgttt
    85381 gtttaaatta tacttttcac ctattgtata gattttcttt agagcttctt tgctcaaaac
    85441 cttcttatac tcatatccca gtaatttaaa atatgtccca ttagttgata taatatatat
    85501 atcatcccct aaaacacttg taaaatattt catagcatta taaggtcttc ccgtaactaa
    85561 ggctaccttg ataccctttt ccatggcttc ttttagtgct ttaatatttt cttctgatac
    85621 ttcaaatcct ttacccatca aagtaccatc catatcagta caaattaatt tatatcgcat
    85681 ttgtatcaca tcctagtaag atttttctaa tatttcaaca aggtcttctt tctttccaaa
    85741 aggtaaaaat cctaaaactg gacagcttgt tatttgttct aattgttcct ttgttacatt
    85801 aaactcagac atttttgtct taagccccac tctctttaaa aaactttcta tttcatttcc
    85861 taaagtttta gctgcttcat catcttccac tttttctaat tcttggttta gtattctcgc
    85921 tactttggca aatttctcta tattatcctt atagaacttt tctgcaaatt ttgggaacac
    85981 tatagccaaa gcctcaccat gaggaatatg tgtaactcct cctattattt cacttagtgg
    86041 atgtggtgct gctgctccag catttgccag tgaacttcct cctaatgtat ctgctactgc
    86101 taactttgtt ctgtattcta tattattttt atcttccatt actttaggta gatagttaat
    86161 gactaattcc atagattttt cagacattat ctctgtaaaa tcattagcat ttggatttat
    86221 ataactttca aatgcatgag taaaagcatc aaatgctgtt gatgcagtaa ttctctctgg
    86281 cagagttaac ataagctctg ggtcaactat acattctttt gagaaacaat tttgatgaaa
    86341 tattgtattt ttctcgcttc ctctcgatat tactgctgct tgtgtaactt ggcttcctgt
    86401 tccagaagta gttgatacag ctattaatgg taattctttt tcacttacct tatcgtatat
    86461 tttaaatgga tttgtatatg tactaaacat ataatcccaa tctatttttt ctaaaccaaa
    86521 agtcaatgct aatacttttg cagtatctat acttgaacct cctccaacag ctaaaacaac
    86581 gtctacagat tcttttttag ctaattcaaa cccttcgttt attaattcta cagttgggtt
    86641 tggctctact ctctgaacct cccatgacta aagtcacagg gttcctgctt cacagaattt
    86701 tgcatactaa aatatgtctt actaattctc cacaggccat ccccgtagtt cctacggttc
    86761 ttacataagt tatttatttt gaattgttat tagtcttaat ccttctttta atatatttat
    86821 acttgcattt atatctctat cgtgagtttc attacaacta ggacaaatcc attctcttat
    86881 atttaagtcc ttcacttcct cgtttttata cccacattta ttgcatattt gtgaacttgc
    86941 aaagaattta cctaccttta caatttgtct accatgccaa ttagccttgt attctaattg
    87001 acgaataaat tctgaccatg atacatctga aattgagcga gatagtttac gatttttaat
    87061 catattcttt acttgtaaat cttctatgca aataatatca ttctctttta ttaatttagt
    87121 agataatttt tg
//
LOCUS       DAMJVK010000004        85359 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.004, whole genome shotgun sequence.
ACCESSION   DAMJVK010000004 DAMJVK010000000
VERSION     DAMJVK010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 85359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 85359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..85359
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.004"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            170..310
                     /locus_tag="PC414_000267"
     CDS             170..310
                     /locus_tag="PC414_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2462682.1"
                     /translation="MGIIESASKLAEMVHLLAVEKGITDIEAWDEAVKEYSKIYEERR
                     NE"
     gene            complement(485..805)
                     /locus_tag="PC414_000268"
     CDS             complement(485..805)
                     /locus_tag="PC414_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HDF2462683.1"
                     /translation="MKLKTISLPELNNLDPTLESTFIKMGEEQGELAECIGKFRNLSG
                     ENNDLDEVDIIKKTAKELMDVAQTCVTMMFKLEEQYGINLDEIRKEHIKKLEKRGYIK
                     NMDK"
     gene            complement(925..1662)
                     /locus_tag="PC414_000269"
     CDS             complement(925..1662)
                     /locus_tag="PC414_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HDF2462684.1"
                     /translation="MDANSLKDLIANHNNIVFFGGAGVSTESNIPDFRSSTGLFSQKL
                     NKQFTAEQLVSHTFFVRYPEEFFEFYKDKLIYPNAKPNNAHIALAKLEEMGKLKAVIT
                     QNIDGLHQMAGSKNVLELHGSVHRNYCTKCGKFFDLESMLNLGGNIPYCDNCGSIVKP
                     DVVLYEEALDSDVITKTISAISNADLLIIGGTSLAVYPAASFIDYYKGDYIALINKAN
                     TVYDKSASLVINKPIGEVLYEAVLRQI"
     gene            1877..3700
                     /gene="acd"
                     /locus_tag="PC414_000270"
     CDS             1877..3700
                     /gene="acd"
                     /locus_tag="PC414_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HDF2462685.1"
                     /translation="MKKKAALATLAMLPLGVVNAHADGDIGIVTINYLNVRNEPTAES
                     SIAFVAKKDDKVLIKDSSNGWYKIKAESGQEGWASSKYIAKSNSDSLRTSTNKEKQVI
                     SNSLNMRNGAGTSYRVITVLKKGQKVEVISESNGWSKIKYDGRLGYVSSSYLGDVSNS
                     TNKSKTKQVNTTSLNVRSGPNTSYGLLGKLPKGSKVEVISEGNGWSKIKYNGKDAYVS
                     SMYLSDVSQSNSDNSSQSNDKKNTDKVVNTASLNVRSGPGSTYSKLGKVYKGSKVTVL
                     SESSGWAKINFNNKEAFVVGNYLSTSADTSNNNSNSNSDNSSNSNGNNSSSSGQVNGM
                     SGISGAKIDYKSLSYTLESHISKQVEKAASGGNVIAPSNRKSTPSPEFSTFSAQRTSS
                     FVNASSSDIEYYLNPKNFTNTTKGMMQFLKINSYRDGISESSLNSYLNGLSSSVFKNQ
                     GAAFINAAKKYNIDVVYLVSHAMWETAYGKSTLAQGQTLTSYKGQPLSKPVKVYNFFG
                     IGAIDKSANVSGAEAAYSNGWTSVEATIDGSAKWISQNYVNSSKYNQNTIYKMKWNYD
                     YTWHQYATDVNWANGISGIMENLIGLYGGGSSLVFEVPQYK"
     gene            3915..8213
                     /locus_tag="PC414_000271"
     CDS             3915..8213
                     /locus_tag="PC414_000271"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HDF2462686.1"
                     /translation="MESIKEYLDKLEINNSGLGKQLKEVYINRVVYFKEDKIVYFYLT
                     SKDIVSHELLDKFKEELMYKLDYFKDMRMKIRFTGLERKSNKDVIKKYWNNILYILKY
                     LCPSIAGWYKQVEFLCLEEELKIKLPKGIFYERLMKKNVTYVLKTVLSEELGLDLNIT
                     IEKAVDEKVNKERLIRINDREMEEKIRALEIGKVNNCEENEEESYVIKSDVDENLIYG
                     DNANAMIENIVELNASSGTVAVVGDIFDVDTKELKNGKILMIASITDYTSSISCKLFL
                     TDTNKDGVLENVKKGAYLKIKGDIVYDTYQREISMMISGIRKETRIEREDKSEEKRVE
                     LHAHTQMSSMDAICSVKKLVERAAKWGHSAIAITDHGVVQGFPDAMNAGKSNNIKILY
                     GVEGYLVEDDSLIIEDANDKELSQTFVVFDIETTGFSNTNDKITEIGAVKIENFKIVD
                     RFSELINPEKDISYKIQELTGITNELIKDKPTIEEVLPKFMEFVGDSVLVAHNAEFDT
                     GFISQKCREQGLLYNNKKVDTLMLARVMLPNLKRHRLNVVAKELGIPLLNHHRAVDDA
                     EATALIFNKFLQMLTEKGAKTLSDVNNILGKIDYTKLSTNHITLIAKNSIGIKNLYKI
                     ISDAHVNHFFRAPRILRSVLNEYKEGLIIGSACEAGVVFQAVKKNVSDEEMKKIIDLY
                     DYIEVMPIDNNRFMIDKGEVKDEEELRELNRKLIDTAKKFDKIPVATGDVHFLDKHEA
                     VLRKVLKYSQGFKVDEEETYLHFRTTDEMLEEFSYLGEELAYEVVVENSNLIADMVED
                     IKPIPDDTYPPIIEGSDVELREMCYKKAKRIYGDPIPEIVQKRLDRELNSIISNGYAV
                     MYIIAQKLVAKSLSDGYLVGSRGSVGSSFAATMSDITEVNPLPAHYICENEDCKYSFF
                     YEIGEWGSGVDLPDKDCPKCGRKLAKNGHDIPFEVFLGFEGDKEPDIDLNFSGNYQPT
                     IHKYTEELFGEGYVYRAGTIGTVAEKTAFGYARKYVEENNISVPNAEVLRLSNGCTGV
                     KRTSGQHPGGVMVIPDYKDVYDFTPIQYPANDTSCGVITTHFDYHSISGRILKLDILG
                     HDGPTIIRMLEDMTGINITEIPLDDKETMSLFTSTEALGVTPEEINCPIGCLAVPEFG
                     TKFVRQMLLDTKPTTFAELVRISGLSHGTDVWVNNAQDLVREDIVGLKDVISTRDDIM
                     NYLIFKGLPPKMSFTIMESVRKGKGLKPEHIEEMKKNNVPEWYIGSCKKIKYMFPKAH
                     AVAYVMTSFRIAYCKVHYPEAFYATYFTTKVEDFDADLIVKGLDAIKSKINEIESLGN
                     DATTKEKGMLTVLEVALEMYARGIKLLPVDIYKSDATEFIVVGEKTLLPPMAAIQGLG
                     GNAAINIQNERKNGEFISKEDLRKRTKISKTVIETLTNHGSLENMSERNQLSLF"
     gene            8417..8878
                     /locus_tag="PC414_000272"
     CDS             8417..8878
                     /locus_tag="PC414_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HDF2462687.1"
                     /translation="MKKNLEATIEEIVTKITDEHGFEMVDVEYVKEAGEYYLRVYIDK
                     EEGISLNECELVSRELSPILDEKDPIKENYFLEVSSPGLDRALKKDRDFVRYQGRDVD
                     LKLYKPLNGCKQFEGELVGLTEDNNIKIIANGKEMEFNRKDVAIVRLAIKF"
     gene            8908..10062
                     /gene="nusA"
                     /locus_tag="PC414_000273"
     CDS             8908..10062
                     /gene="nusA"
                     /locus_tag="PC414_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HDF2462688.1"
                     /translation="MNHEFMEALDELVVDRGIDKEILIDTIEQALLTAYKKNFGSAQN
                     VRVEFDRDKGDIKVFSQRVVVDESDLYDTFLEIELADAREINPNYELGDIIEHEVTPK
                     NFGRIAAQTAKQVVVQRIREAEREIVYNEFIEKENEIVTGEVARANKNVVYVNLGRIE
                     GIMTQSEQIPFENYKAGQKIKFYILEVKKTNKGPQIVVSRSHPGLVKRLFEEEVPEIY
                     EGIVQIKSVAREAGSRTKMAVRSIDEKIDPIGACVGPKGLRVKNIVDELGDEKIDIIK
                     YSEDPAEFISAALSPSKVVRVDIDEDEKSALVIVPDYQLSLAIGKEGQNARLAAKLTN
                     WKIDIKSESQAEQEMLNLENSKEIEAEDVEAEFIDQEELDTSEDNFSEEE"
     gene            10069..10350
                     /locus_tag="PC414_000274"
     CDS             10069..10350
                     /locus_tag="PC414_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HDF2462689.1"
                     /translation="MILKKVKKVPQRKCIACQDRDSKKELIRIVKNKEGKIFLDPTGR
                     ANGRGAYVCKSSECLKKAIKSKALNKAFKIDVPDEVYDNLLMELEEYEK"
     gene            10340..10651
                     /locus_tag="PC414_000275"
     CDS             10340..10651
                     /locus_tag="PC414_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HDF2462690.1"
                     /translation="MKNNKEKVLSFLGLATRAGKIVSGDDSTLLDLKKGKVNLILIAE
                     DASNNTKKLFKDKSTFRNIPYLFFSTKEEIGFAIGKSPRAVVGIKDENFSKKIIELIE
                     I"
     gene            10673..12613
                     /gene="infB"
                     /locus_tag="PC414_000276"
     CDS             10673..12613
                     /gene="infB"
                     /locus_tag="PC414_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDF2462691.1"
                     /translation="MSKTRVYQIAEELNISNEELINKLAELDINVTDKDSVLEGEELE
                     LALEMLGEDLSQENGNVIEIDGKLTVQVLATKLDKSPSEIIMKLMKMGTMATINQEIS
                     FEIAALAAKDYGFELTVAESDDTEALEIEALMEIEEDKEEDLKPRPPVVTVMGHVDHG
                     KTSLLDAIRKTDVISGEAGGITQHIGASEVKINGHKIVFLDTPGHEAFTSMRARGAQV
                     TDIAILVVAADDGIMPQTVEAINHAKAAGVPLIVAINKIDKPGANPDKVKQELADQGL
                     LVEDWGGEVIAVPVSAKKKEGIDTLLEMVLLVAEMEELRANPNKRAVGTVIEAELDKG
                     RGPVATVLVQGGTLTVGDPIVAGVACGKVRAMINAKGKRVKTAGPSTAVEILGLSEVP
                     QGGDQFVEVPTDKIARSVAARRQQIVRDEMLKSTQRLSLDALFSQMSEGSIKDLNIVI
                     KADVQGSVQAVKQSLEKLSNEEVQVKVIHGGVGAVTESDILLAAASNAIIIGFNVRPV
                     PGAESLGEKENVDIRTYTIIYKAIEDIQAAMTGMLDPEYVDEETGKAEIREIYKISGV
                     GTVAGCYVTNGKIFRNCKVRLVRDSIIIHEGELAALKRFKDDVKEVNSGYECGMSFVN
                     YNDIKEGDIVEAYITKEVERKL"
     gene            12656..13036
                     /gene="rbfA"
                     /locus_tag="PC414_000277"
     CDS             12656..13036
                     /gene="rbfA"
                     /locus_tag="PC414_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HDF2462692.1"
                     /translation="MASYNRTRRIAEEIRKVVSTMLINGVKDPRITSMVSVTDVEVTN
                     DLRYAYVYVSILGGDEESTLTGLKSAGGYIRREVGKNIKLRYIPEIVFKLDDSIEKGM
                     YMDSLIKRVNEKNAQQNEDENYDE"
     gene            13017..14000
                     /locus_tag="PC414_000278"
     CDS             13017..14000
                     /locus_tag="PC414_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HDF2462693.1"
                     /translation="MKIMMNNMDSIINYIYEGDYFIVTSHISPDGDNIGSTLSMYYTL
                     KNLGKNVYYVLDDEAPLNLRFLVEDVKIYKSNEFNMKNYSLIALDCGDKMRICVSDEI
                     KNNASKLICIDHHASNDSYGDLNYVDIDASSTCELVYNLLVRFQQIKDINIINEDIAT
                     CLYTGLVTDTGNFSYSNVHASSFEMAKNLLVLGAQKNTIIQNIYQSNSSGYYKLLGEA
                     LKGLEIFDSKVSSIVLTQDMMNRNNISFNDVDGITTYTRDIKGIEVGILFKEKKQNEI
                     KVSFRSKNYVDVSEIAKLFGGGGHVRAAGCTIRDSIDNAKKMVLEAVLKSI"
     gene            14013..14918
                     /gene="truB"
                     /locus_tag="PC414_000279"
     CDS             14013..14918
                     /gene="truB"
                     /locus_tag="PC414_000279"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDF2462694.1"
                     /translation="MNKIISILKPTGMTSHDVVSRVRKILNIKKVGHTGTLDPDASGV
                     LPICIGKATKVCEVILNKDKSYICELTLGISTDTYDASGEILKKVDDFKFSNEDIERA
                     FDTQRGEINQLPPIYSALKVNGKRMCDLVRSGRQSEITLKTRRVNIKDIKILSIKGNK
                     VMFYVECSKGTYVRSICHDIGEYLGCGAHMSFLNRTSSGKFDLDNSITLEELELFYEN
                     KTLDKYLYDIDYVLDSFNYVVLNPNAIKYYSNGGSIDDKRFLKNNFDKDDEFVRVYST
                     DNFLGLGKLSKHNNTISVKSDKMFI"
     gene            15049..15978
                     /locus_tag="PC414_000280"
     CDS             15049..15978
                     /locus_tag="PC414_000280"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HDF2462695.1"
                     /translation="MVIIKSIKEIANIKESVITIGNFDGVHKGHQVLIGKTVEHAKKE
                     NIQSIVFTFANHPVNYFRPNSTKNIISNDDKIKLLEDLGIDIVVIIPFDEYMTKIPAK
                     DFVDEILVKKLKAKKIVIGHDFTFARAKEGNVNLLKSLEHEFGFEVEVIKPIKINDVR
                     VSSTYIRSLVSQGDMANVKEYLGRNYKLEGCVIHSKHLGRTIGFPTANIDLKNNMLVP
                     KRGIYASIVHIGDETYFGATNVGYNPTVNGKTLSIETNILEFDRDIYGENIIVEFLER
                     IRDERKFNSIDDLKNQLYNDTNYVYENYVCKNK"
     gene            16082..16339
                     /gene="rpsO"
                     /locus_tag="PC414_000281"
     CDS             16082..16339
                     /gene="rpsO"
                     /locus_tag="PC414_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HDF2462696.1"
                     /translation="MINKSEIIKEYGRAEGDTGSPEVQIALLTARINELNGHLKTHKK
                     DHHSRRGLLKMVGRRRNLLAYLKEKDLEGYRALIAKLGLRK"
     gene            16579..17424
                     /locus_tag="PC414_000282"
     CDS             16579..17424
                     /locus_tag="PC414_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDF2462697.1"
                     /translation="MKIKLVCDSLCDIPDEISEKDYIEIVPLTVIFNDREYIEGVDIK
                     KEEFYKKVKEIKQIPKTSQATYMEFKEVFDKFITEGYHIICMTGAANASGTFQSAMIA
                     KNDVNENEKIHIFDTRNLSLGSGQYVIKACELLEEGLGFEEIIDELENTRSSVRLLFA
                     PYTLDFLKQSGRVPVATALIGNMLNIKPIFFFDNGEAKLVNKVRGIKNIASKLVDIIL
                     EMNEGSLEGKIVTIGCGDNLHDCEILKDEVNKRIKARRVLFTRGGVSICSHTGPDILA
                     ISCSN"
     gene            17549..19660
                     /gene="pnp"
                     /locus_tag="PC414_000283"
     CDS             17549..19660
                     /gene="pnp"
                     /locus_tag="PC414_000283"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HDF2462698.1"
                     /translation="MFEHKIFKMDFAGRELSVEIGKICEMASGSCIVRYSDSMVMVNT
                     TKSAKPRDGIDFFPLSVDYEEKLYSVGKIPGGFLKREGKPSEKAILTSRLIDRPIRPL
                     FPKGFRNDVQVVATVLSVDQDCTPDIVAMIGSSIALSISDIPFNGPTGSVCVGLVDGA
                     FVVNPNAEQREKSSMHLVVSGTKEAIMMVEAGADEVPDEVMLDAILFAHQEIKKIVEF
                     IEGIVAEVGKEKMPVELYHAGEEITQLVREFATDKMKKAVQTFEKLERMENMDRVKEE
                     TLAHFEETLEDFEDFVGDIEEVLQDIIKEEVRKLIVHENVRPDNRKLEEIRPIWCETG
                     MIPRAHGSAIFTRGQTQVLNVATLGALGDVQKLDGLDEEENKRYMHHYNFPAYSVGEA
                     RPSRGPGRREIGHGALAERALLPVIPSQEEFPYAIRLVSEVLSSNGSTSQASVCGSTL
                     SLLDAGVPIKDMVAGIAMGLIKHDGKVAVLSDIQGMEDHLGDMDFKVAGTEYGITAIQ
                     MDIKIDGIDKEILQRALKQAKEGRIHILGEMRKTISQPKPELSPYAPKIVKMQINPDK
                     IKDVIGPGGKIITKIIDETGVKIDIEQTGEVFISGIEIDMIKKAQELINNIVVEPEVG
                     KTYKGKVSRIMNFGAFVEILPGKEGLLHISHIAHERVAKVEDVLNIGDEVEVKVTEID
                     EKGRVNLSRKVLLPKPEHKNK"
     gene            19926..20669
                     /locus_tag="PC414_000284"
     CDS             19926..20669
                     /locus_tag="PC414_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2462699.1"
                     /translation="MIMMVLNKSKLKKIIILILVVLLVLSGGVMVLKLFNNKPVFNLF
                     NGVDLPYERGTKDKSGYVALTCNIDLGWETEYVESILETLKKENVKITFNVTGKWAEK
                     NKDELLKIKKQGHEIGNHGYKHLDYSTLSYEDNYEQIETSKKIIEEIIGEKTKFFQAP
                     AGSFGPETVKAAKALGYTSIKWDADTIDWKYKDQPEVIIDRMKKKDIKDSSIILMHPT
                     NATTKCIDDIIAIVREKGLKAGKLSDVFK"
     gene            20827..22074
                     /locus_tag="PC414_000285"
     CDS             20827..22074
                     /locus_tag="PC414_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDF2462700.1"
                     /translation="MYKTKILENGLTIIGEEIPYLKSITLGIWINAGSRIEEAQVSGT
                     SHFIEHMMFKGTKNRTSKEIASSIDNLGGQINAFTSKECTCYYVKLIDEHIDTGIDVL
                     SDMILNSKFDKNDIDKERLIILEELKMYEDSPDDLSYDLLVENIYANDGLGMNIIGTK
                     ESLYNITRESMLEYLNKYYIPNNAVISIAGNFNFDDMVEKIKSKFGHWEKKNLSIDIS
                     EAKFNPCFISKNKDTEQVNLAMCLKGIPFENDEEVYSMAVVNNIFGGSISSRLFQKIR
                     EEKGLVYSIYSSQTLYRKCGELGIFASMSTENLQDVYNLIKKEIENIRENYLTEKEIS
                     ESKEQLKGNYILDLESTSSRMMSTGKSMLLSKKVKTTDEILECINSVNINSIKKVVDK
                     VFNIENIGTCIVGRDVEKILHLD"
     gene            22109..22354
                     /locus_tag="PC414_000286"
     CDS             22109..22354
                     /locus_tag="PC414_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HDF2462701.1"
                     /translation="MNLSEIAGKEIVNLVTGERLGVIGECDLILDETTGGILALLIPK
                     EKGFFARRKDKSFLEVPWRNVKKIGNDMIIIEYEGYI"
     gene            22364..23587
                     /gene="dapG"
                     /locus_tag="PC414_000287"
     CDS             22364..23587
                     /gene="dapG"
                     /locus_tag="PC414_000287"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HDF2462702.1"
                     /translation="MSILVQKFGGTSVESYEKMNEVCKIVKAYKKNDEELQLVLVVSA
                     MGRKGAPYATDTLINLCSAVNDEPSKRELDLIMSCGEIISGTILANLLNAQGIDSVFF
                     TGQQAGIITSDEYSNAKIKYINPKKVKRALDDGKVVVIAGFQGVTDDGEITTLGRGGS
                     DTSAVAIGKALECETVEIYTDVDGIMTADPRVEPNAKVLSFIDYEEVFQMADKGAKVI
                     HPRAVQLAKSGNITLAIKNTMNPTFEGTKIGSLCRHLEDNIEYEQERDFKVAVANKDS
                     VAQVKIKSAEEVFTEVLNEIEKKLITIDMINFFISEKAFVVEDADIKSLKEILDKFEL
                     DYEVNRDCAKVTLICSRIADEMSGIMSKVVRGLSKAGVSLLQTSDSNMTISCLVSEED
                     MHTAVHAIHQQFYLK"
     gene            23700..24434
                     /locus_tag="PC414_000288"
     CDS             23700..24434
                     /locus_tag="PC414_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HDF2462703.1"
                     /translation="MKDYREDNERFDKDNCSEESSCEERECIKQFGTNEMPPQPPKDI
                     QCITIIGEIEGHFIGNPQKKATKYEHIIPMLYSIEESNDVKGVLVVLNTVGGDIEAGL
                     AIAELLNSTSKKVVTLVLGGSHSIGVPLATAGDYSFIAPTATMIIHPVRTTGLVIGIN
                     ETFEYFKKMQDRIIQFIIRTSNIKKDVLEKLMHEKDELVSDVGSVLIGKEAVDYGLID
                     EVGGLKEALKKLRELIKESEEEKNND"
     gene            24557..26968
                     /locus_tag="PC414_000289"
     CDS             24557..26968
                     /locus_tag="PC414_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDF2462704.1"
                     /translation="MATKKKKKKKVNTLSFNAEYHNLITIFIGVFLLYSLNSNSMGWI
                     PVLMQNLFKGLFGGLSIAIPFIVIITGLLGFFDGNEYIYRLRKTKLYYIIILFIFVFY
                     GLLNAGTLPVDSPLKGNMFDDVMKLGVSGQGSGLIATTIAYYMSKIFGIAGGWLISIF
                     ALILSVMFIFNISIKDLVSNAKSKSKASKDSNLTFKDKIANMKKSAIDMMTDEVDDTT
                     INKKPGFFKGLMSKGLNNDDEEDEYFEEENSDGVDDKTIKIVGFNKAEDEYLEILEGT
                     QSMPELDVLKELQKATNENPVVDTKPEKKVDIAKPNLNIEKTQPMSIVAEPVNEDYSN
                     YKKPSIELLNKVNKKSDENGKKKVLKNASLLEKTLSDFGVEAKINQVTVGPTITRYEI
                     QPSPGVKVSKIVNLTDDIALSLAAKSIRIEAPIPGKSAIGIEVPNEEAQMVGVREVLE
                     SEEFNNFNSPLAMGLGKDVAGKIIIGDIGKMPHLLIAGSTGSGKSVCVNTLISSILYK
                     ANPDEVKLLLIDPKVVELANYNGIPHLLIPVVTDPKKAANALNWAVTEMNRRYKLFAD
                     AQVKDVTSYNEKAEEKLPKIVIIIDELADLMMASANDVEDYICRLAQMARAAGMHLIV
                     ATQRPSVDVITGVIKANIPSRIAFAVSSQTDSRTILDMGGAEKLLGKGDMLFYPLGAA
                     KPVRLQGAFISESESEKVIDFVKSQVKDGIKYEEDIIETISKVNTSKGSDEDEFLSEA
                     IEFVVESGQASASMLQRRFKIGFNRAARLIDSMEERGIIGASEGSKPRKVLISKQDLQ
                     NLEGE"
     gene            26971..28029
                     /gene="mnmH"
                     /locus_tag="PC414_000290"
     CDS             26971..28029
                     /gene="mnmH"
                     /locus_tag="PC414_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HDF2462705.1"
                     /translation="MSVIKIEKALKLDKVIFVDVRTEGEYEEDHILNAINMPLFKNNE
                     HNDVGTIYKMQGKHEAIQKGFDYVSYKLKDIYLQAAELALNYDNIVIYCARGGMRSGS
                     IVNLLSSLGVNVYQLEGGYKAYRNFVLEYLRHVMDTKNFVSVHGLTGTGKTDLLHLLD
                     EKGIDVLDLEGIAKNSGSTFGFITFDKKPPSQKNFETKLFESIFFSKENYIFVESESK
                     RVGSVTVPNEIFEAMTREGHHILLECDIENRVDRLCRDYIYDKDEGNILILKECINKF
                     RKRLGNKVVDDYIDLLESGEYKELVKRYLLEYYDPLYMHSVEKYKYSKIINFNDTQKA
                     LEDVIDLYESILEGESEC"
     gene            28023..29357
                     /gene="rimO"
                     /locus_tag="PC414_000291"
     CDS             28023..29357
                     /gene="rimO"
                     /locus_tag="PC414_000291"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HDF2462706.1"
                     /translation="MLKIALESLGCSKNLVDAEIMMGILNNKGYKLIGDFEEADVIIV
                     NTCGFIESAKQESIDTIINFAELKKTGNLKLLIVTGCLAQRYSEELKTEIPEIDAIVG
                     TGSYQNIDKILKELSEMHKIVSLNDIEFVFNEDLPRYISTPSYMAYLKIGEGCSNNCT
                     YCIIPKLRGKYRSRKFEDIIKEAKKLAESGVKELVVIAQDTTKYGFDLYGKERLSELL
                     EELAKIDGFKWIRVMYSYPESITEELIQVIKKYDNICSYFDMPIQHASNNILKLMNRK
                     TTKEDILNKINLIRSNIPDAILRTTIIVGFPGETEDDFKQLVDFVEEVKFDRLGAFAY
                     SREEDTPADRLPNHIDEEVKIQRRDTLMMIQQKISEELNDKKIGKTYEVLIEEQIEDN
                     VYTGRTQGDAEEIDSIVYVKSVDNLEVGEFVSVQINDAMEYDLMGDVLYELA"
     gene            29344..29886
                     /gene="pgsA"
                     /locus_tag="PC414_000292"
     CDS             29344..29886
                     /gene="pgsA"
                     /locus_tag="PC414_000292"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HDF2462707.1"
                     /translation="MNLPNKLTLFRIFLIPVFVLIMLLNIPNKFLIACIIFIIASITD
                     ALDGKIARKYNLVTDFGKFMDPLADKLLVISALTCMIEDHLVSSWMVIIIVARELTVS
                     ILRAIAAADGKVIAAGNSGKLKTITQMVSIVFLLLGAQFENVLILNIGEILILIATLL
                     TLYSGWEYLYKNKELFMSSK"
     gene            30174..31220
                     /gene="recA"
                     /locus_tag="PC414_000293"
     CDS             30174..31220
                     /gene="recA"
                     /locus_tag="PC414_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDF2462708.1"
                     /translation="MSVDQEKLKALNEALGKIEKDFGKGSVMKLGEATSMSIDVISTG
                     AIGLDIAIGIGGLPRGRIVEVYGPESSGKTTVALSCVASAQKDGGIAAFIDAEHALDP
                     VYAKALGVDVDNLIISQPDTGEQALEIAEALIRSGAIDIIVIDSVAALVPKAEIDGDM
                     GDSHVGLQARLMSQALRKLTGSIKKSNCVAIFINQLREKVGIMFGNPETTTGGRALKF
                     YSSVRLDVRKIDTIKQGDKVIGSRTRVKVVKNKVAPPFKQAEFDIMYGEGISKIGDLL
                     DIAADVDIVKKSGSWYSYNDTKLGQGRENVKKFLEDNLDLTTEIDEKVRAFYNLNEEH
                     EESGTSVSKEIVEE"
     gene            31396..32937
                     /gene="rny"
                     /locus_tag="PC414_000294"
     CDS             31396..32937
                     /gene="rny"
                     /locus_tag="PC414_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HDF2462709.1"
                     /translation="MDVIDSVVMIVIGAAVGIIAGYFVRKNISEAKIGQAENLAKEII
                     DKAHHDSETVQKEKLLEAKEEIHKWRTEAERENRERRTEVQKYEKRVVQKEEVLDRKL
                     QNLESKEVNLSEKLKVVEKKEEEVEVIKTQQLEKLESISGITSDKAKEIILTNAERDV
                     RREMSIMIKEIESQAKEEADKKSREIIGYAIQKCAADHVAETTVTVVSLPNDEMKGRI
                     IGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARIALEKLIADGRIHPAR
                     IEEMVEKARKEVDNIIKEYGEQAAFETSVHGIHPELIRLLGRLNYRTSYGQNVLKHSI
                     EVAHIAGIMAAEIGADIRLAKRAGLLHDIGKAVDHEMEGTHVEIGMDLLRRYKESKEV
                     IHAMSTHHGDYEPQTVEAVLVTAADAISAARPGARRETLEAYIKRLEKLEEIANSYEG
                     VEKSFAIQAGREIRIMVKPEAINDEEIHLLARDMTKKIEDELEYPGQIKVSIIRETRA
                     IEYAK"
     gene            33251..35587
                     /locus_tag="PC414_000295"
     CDS             33251..35587
                     /locus_tag="PC414_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF2462710.1"
                     /translation="MKKNTLLHTNIMVCIIITLGFVATSIIGYQSNTNIFKNDIEHVS
                     TLAAEGIYYQIDKLLSEPINVSLTMANDSLLKNFLDGEKEHLNDEEFIYKLQDYLNVY
                     RNKYSYDSVFLVSTKTNNYYHFNGLDRNLSANNSENQWYYTFLKNDDEYSLNVDNDEA
                     SNNSITVFVNCKIKDDNGATMGIIGVGLKVNSLQMLLKGYNDKFDVVARLIDDKGFVQ
                     LAVDKTGHENINFFENSSSDLSNSKQLILNNKKEQKSFWYSSEKSKSYIVCQYIPSLK
                     WHLVLENDFTLMIKQLHLQFFKGIAVIVLIIIFVLFTITHVLKKYKNQIIKLSVSQKL
                     EYQKLLSKSTEGLYENIFEIDITNNKAGCEDENRYFHEFGLGLNTPYDEALHTIAKNQ
                     IKEEYAQGYLDTFLPENVIKRYNSGINNISYDFLIKKDEESYHWIRINARIFFWTSDE
                     SVRMISYRKNIDEEKNRELLLLERSQRDSLTGLYNKATTEEFIRSFLKNESNQDLEHV
                     FLIFDIDNFKNVNDKYGHVFGDFVISEFALELKSQFREDDIVGRIGGDEFVVLIKDFN
                     NNNYVLIDKLERFCSKINQKKFVEQAEFSIACSIGVAMYPEHGYVYSELYEKADQALY
                     YAKSHGKNSYHIFSEDSIDSISFHIDSRDIKVLLEMATDGVVKFACMENLKLLYFNQK
                     MLEFTGYSAQTVTSIGFDIFSPVLQDDLQMVLDTLREAISKRETFEIVFRFCHTDGHI
                     FPVRMQGKFVSELYQNRYPVFYAIYTDISSINTLLENK"
     gene            35969..37750
                     /locus_tag="PC414_000296"
     CDS             35969..37750
                     /locus_tag="PC414_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HDF2462711.1"
                     /translation="MSQYININSQLDALKLKEFEKIFYENINEDVKVVPKVTPFKGIN
                     TDLLYVKDGRILFIKFMDTTEDIFFILEEELLEVMNEEYELLKLKMGQKNRNISYNYV
                     YIMPYVEVEETYEFEEFVNNNIIDKNKLQDIMNKGSLEEYLNDENDEINLNLFLLDIC
                     SEYYIINDKLHLNEKFKKISFYNDDYKYTATMMEDVQIKDVVSIKYGNTLIEGGSGIG
                     KTAIMLSRAIKLAKVYPHHKLVIFTHTKQLCNELRERIELLYKDNNNLEVHTFSSFIF
                     KLAKKFNLIVDYNMLKNDYEKTFNNLVKQAQNIIKNKNMFKAIFIDEAESFLEYEIDF
                     IREFLYKTKFIFNVCSCNSLNISNRLNIFKKLYNGIEFDDKIILSKNYRQAKEIVDFT
                     NKFSNNSNSYINELRPNTEFSTFFYTKALRGGNKSVDIIKVSDLDDQISSVIWEIEYL
                     ISKKGLDYSEVAIVYPYNKKKLKSGKTIYFQYMLKKALEEAKIPYICAEDNLTNISKK
                     VGTTIANIYAIKNLEYKAVIVCELEMLYNQTINDIEQDYQVNDFVGDLNKVYLAMSRA
                     TDYLSIVTTFNEEASDIIRLITESKDI"
     gene            complement(37832..38362)
                     /gene="lepB"
                     /locus_tag="PC414_000297"
     CDS             complement(37832..38362)
                     /gene="lepB"
                     /locus_tag="PC414_000297"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDF2462712.1"
                     /translation="MSVKKEIFDWIKSIAMAIVLAFVILQFIRPSIVSGESMYPTLDD
                     KDYLILNRISYKVGKPEKGDIVVFKTNLVDGETGKKKDLIKRVIATEGDRIKISNSKV
                     YVNGKLLNEPYIHNNYTSGDIDTVVPKGKLFAMGDNRENSNDSRFPDVGMVDEDEVLG
                     KVMVRLLPLDNIGKVD"
     gene            complement(38402..39226)
                     /locus_tag="PC414_000298"
     CDS             complement(38402..39226)
                     /locus_tag="PC414_000298"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDF2462713.1"
                     /translation="METFSLLEIFKAVILGIVQGITEWLPVSSTGHMILVDEFIKLNF
                     SNTFISTFLVVIQFGSILAVLVIFFRKLNPFDSAKNIKQKKETVRLWLKVIIAVIPSG
                     VIGILFEDDIDRLFFNSTVVAIALIVYGIIMIGLEKRNKKPKYKDFSQVTYKLALCIG
                     LFQCLALIPGTSRSGSTIIGAVLLGTSRYVAAEFSFFLAIPTMLGASALKLLKAGFGF
                     TGFEWLILGVGSVVAFVVSIVVIKFFMDYIKKHDFKVFGYYRIVLGIVVLAYFFLL"
     gene            39483..40391
                     /locus_tag="PC414_000299"
     CDS             39483..40391
                     /locus_tag="PC414_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF2462714.1"
                     /translation="MYSTKILDDYEYETFNVFSTKLNPNTKHDYLSKVILFKEFLKGK
                     ELIYATKEDCKNFVDYIQTKYAKSTCEKIYSYLHSFYNFLKKEGYIDINPFRYVEKPT
                     VTRIKTKDDVLSIQEINKLIGILPKLNIRDRVIIVCLVTTGCLLNELVSLKWKDLMVD
                     ENDNSYVRLGKGRKERVVKLHPYFFKLLEDYRNYSGLPEVIIPSDDFIFTTQKSNSIT
                     DRNVRLIVKKALDLAGLSQYSARDFRHSFAAISLRLGADESDVKNQLGWSDKYYAIRY
                     KYVLNFVDSEIVDYLIEKDNLDINKK"
     gene            40535..41980
                     /locus_tag="PC414_000300"
     CDS             40535..41980
                     /locus_tag="PC414_000300"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HDF2462715.1"
                     /translation="MIDNKYTTYSNPLTDRYCSKDMSYIFSPQFKFSTWRRLWVALAE
                     GEHELGINITEEQLDELRANIDNINFDEARKIEKEVRHDVMSHVKAYGLQCEKAKGII
                     HLGATSAYVGDNTDVIQMNEALKLLRVKLVNLINNLKEFALKYKDMPALGFTHFQAAQ
                     LTTVGKRACLWAQDLILDLEDLNYRIDNMKLRGVKGTTGTQASFLSLFEGDHEKVKEL
                     DKKICKKMGYHSSYAVSGQTYTRKLDYQVISILSGIAQSMHKMTNDIRLLQSLKELEE
                     PFEKNQIGSSAMAYKRNPMRSERIASLSKYIISESISPAMVQATQWLERTLDDSANKR
                     LAIPQAFMAADAILEIGINVTDGLVVYENMINRRVNEELPFMATETILMEAVKRGGDR
                     QELHEIIREYSMKAAYRVKHEGKDNNLIELIMNDDSFKMSKEEILSIMDPKNFIGRAP
                     EQVVEFVNEVVEPAIKDYKEDLGKVDVDLRV"
     gene            42291..42743
                     /locus_tag="PC414_000301"
     CDS             42291..42743
                     /locus_tag="PC414_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2462716.1"
                     /translation="MNYIVKDTISFLVKTFPKIYSSLYLEDLKKFAPDYNVNKTQLRA
                     LVFIKNYGVISMTDLCSKLNIEKGSLTSMVDDLTDKKYVTRKRDLVDRRKYLIDITEE
                     GDKIATDFMDKLSDGLGEKLSKLTEEDRKKYLEAINTLQYILNKEEFR"
     gene            complement(43078..44274)
                     /locus_tag="PC414_000302"
     CDS             complement(43078..44274)
                     /locus_tag="PC414_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2462717.1"
                     /translation="MNYSNRVSAMQASPIRKLVPFAQAAKDKGIKVYHLNIGQPDIKT
                     PKGFFDAVKNFDSEVLEYATSEGIPELLEALQNYYKTYDMNFEKDELLVTNGGSEALL
                     FTMMAVCDPGDNLLVPEPFYTNYNGFGQSVNVEVNAVTTKAENGFHLPSKEEILSKVD
                     DKTKAIILSNPGNPTGAIYTKEELNILAEIAKEKDLWIIADEVYREFVYDGLEYTSCG
                     NLEGVQDRVIIIDSVSKRYSACGARIGSIACKNKGLIAQILKLCQGRLCVPTLEQIGA
                     VELYKTPVSYFKEVNEEYKKRRDVLYNELMKVEGVICKKPTGAFYIVAKLPVENAEDF
                     TIWMLKEFNKDNETVMVCPAEGFYATPGLGRDEIRLAYILNEKDLHRAATLLKEGLEQ
                     YVALQK"
     gene            44659..45609
                     /gene="galT"
                     /locus_tag="PC414_000303"
     CDS             44659..45609
                     /gene="galT"
                     /locus_tag="PC414_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HDF2462718.1"
                     /translation="MKELRIDPITNDVVIFATDRLKRPLDTADIPNEDEETNEYDEEC
                     PFCKGNEAHATDALFEIDSEEGWLVKSVYNKFPIIDDMARDVYGVHEVMIESDKHNRS
                     FYNMSQKEFEDVFFMYRNRFRDLSKDDKVEYVSIFKNFLRKAGASLMHPHAQILSMSF
                     IPPEITNELLVSKEYYDSNKSSLYDDLIENEISLDRRVVYNGEAFLVLIPYATKYSGE
                     VRIIFKDKIEFGELNDNNIQELSTIFEKLFKKLYNINGYMPFNLCIHTHPTKIETKSY
                     FNVHMHIIPRKYNFGGFELGTNMYVSSMSPEDLTKKLKID"
     gene            45770..46522
                     /gene="xth"
                     /locus_tag="PC414_000304"
     CDS             45770..46522
                     /gene="xth"
                     /locus_tag="PC414_000304"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HDF2462719.1"
                     /translation="MKFISWNVNGIRACVGKGFLDFFKEVDADIFCLQETKLQEGQIE
                     LDLPGYFQYWNYAERKGYSGTAIFTKKEPLKVMYGINIEEHDKEGRVITLEFEDFYFV
                     TVYTPNSQSELKRLEYRTRWEDDFIDYLTKLDNHKPVIVCGDMNVAHKEIDLKNPKNN
                     MKNAGFTKEEREKFSKLLDSGFIDTYRYFNPDKEGVYSWWSYRFNARKNNAGWRIDYF
                     CASKKLEDRLVSADIHTEILGSDHCPVELEIK"
     gene            complement(46614..47390)
                     /locus_tag="PC414_000305"
     CDS             complement(46614..47390)
                     /locus_tag="PC414_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HDF2462720.1"
                     /translation="MEYIIFDLEFNQGFDKKLNKTVSNEKCPFEIIQIGAIKLDSKFN
                     IIDTFNSYVKPTIYKDIHPFISRMTNIKSSDFNDSPTFPEVYNNFIKFISSQDPILCV
                     WGAGDLKELYRNINYHKLPSNSLPKSYINIQQHASKYFNNPAGKSIGLQNAISILELD
                     EKMSYHNALNDAYYTAKVFIKIYNPSIVPDIYLYTSIKPKTIRYSNKKRVDYDKLFDE
                     FRKILNRELTKDEKKIINLAYNMWKTNQFTLENVKQRKNK"
     gene            47705..49603
                     /locus_tag="PC414_000306"
     CDS             47705..49603
                     /locus_tag="PC414_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HDF2462721.1"
                     /translation="MDSLLYVIEKDKHTNEELREILKSNKNIRFVSLMGVDLGGNATD
                     EKIPVELFLEDIDKFLESAIQTDGSSVELYNIATLNNAKVDLMPDKSCHWYVDYNMEY
                     IDEEVGLPVGTLKIPAFLIHDNKKVCSRGVLQKADKYFKKSMYEIFREYPHVINNIGI
                     DSVDDIEEIMLTAATELEFWVNTPEDKADLEKLYVSQSLKEQYWKRTHGIIRTCLEKS
                     LIILQKLGVNPEMAHKEVGGIQSSISIDGRTNHAMEQLEVSWKFSTPLQAADNELLVR
                     DVIEDVFTSHGLEVTFKAKPIHGVAGSGGHTHVGVSAKLKDGSIKNLFAPKDLKEDYL
                     SELGYGALMGLLYNYEVLNPIVTASNDGFNRLVPGFEAPVCIVTSLGHSYEIPSRNRS
                     VLVGLIRDMKNPKTVRFELRSPSPLSNTYLVIAGCYQTMLDGIKAAAKSGLSTKELEK
                     ELSKNVGEESFYLEKDRAYRDENDVFEHYSLEERNARFGIPPATVYENMKNLEIYASK
                     LKSLKQGDVFTDSIIESFKIGAIDKWQKKLKTRIIEAGIQKIRSIVKIHTKENMDALD
                     EVVWNSISDLKFNIMKDTLTRESLFTRVREAIENKDYQTASDLQIELKRSMEEIQQLY
                     MQYKKNIY"
     gene            complement(49731..50852)
                     /locus_tag="PC414_000307"
     CDS             complement(49731..50852)
                     /locus_tag="PC414_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosyldiacylglycerol synthase"
                     /protein_id="HDF2462722.1"
                     /translation="MKDDKTILILTAQFGAGHISAAKAVKECIIEKYSNYNVVIQNFI
                     NASIPMMNKPMVKLYENNTKYTPGLYNYYYYFKKSFDSRHDLSHKLYTPKLSEYIADI
                     NPDLIISTFPLAAACVNNFKIKNPDINIPTLTVITDVVDSMEWVFENTDLYFVPSPEI
                     KNRFFQKGINPDSIKVTGVPVDKRFQIESKELCCDKYRLLLLGGGRGLFDIDEDFMHW
                     IDEFIEEHSNSIEITIVTGKNKKLYDNLTHKKPLKNIKVLGFVNDMYNLIRECDLMLT
                     KPGGATIFEAIQSQTPVLVKMPKVGQEIENAKFIIDKGLGMIYSDDLDLKNIFYKLVS
                     NEFDSIINFMKKNLKEFKTVIHPEKIADYISELIDNHYS"
     gene            51051..51383
                     /locus_tag="PC414_000308"
     CDS             51051..51383
                     /locus_tag="PC414_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDF2462723.1"
                     /translation="MNTQFRKGVLEICVLALISKKDMYGYEIVHNISKVIDVNEGTIY
                     PILRRLTKDLYFETYILESNEGPARKYYRITSLGKENLSNLMEEWREFVKAVEILISE
                     DEGGNELE"
     gene            51370..52428
                     /locus_tag="PC414_000309"
     CDS             51370..52428
                     /locus_tag="PC414_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HDF2462724.1"
                     /translation="MNLNKTEFLEILKDYLKKDFSESEVNDILRDYEEYFVDGLIEGK
                     SDMEIIASLGSPKSIAKELILQTKEKNIEEEKIINTDVLNDKFSKFKSDMKKGYSILK
                     DEAKEKYSKSKENIDGKIMNTDILNGKFSRFKSNVKKDYSILKDEAKEKYNKSKENIN
                     DKLTPSIEEGVGGLSRQVIKGLLVVLSLFLIVPAFAFVCAIVSATIALVCSLIAFVIL
                     IPFVIKFIAMVPDVMMFCIFATIAFVGLEILAWQILLFAIKLGKQLLNNYIHWIKRKN
                     IYIKANEKMEKRKKATLNDEDLNKDINENSYNNDYYNREEESIKETDEYNDDSINFEA
                     IYKDLEDIENKKGEDKHE"
     gene            52421..53491
                     /locus_tag="PC414_000310"
     CDS             52421..53491
                     /locus_tag="PC414_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HDF2462725.1"
                     /translation="MNKKLAIFAAVLLVIGIIGTTWSGILVMPSLINFGLEKEAELNK
                     ENKLYQEKVNIDKLDIAVDNINVMIRKSSNEDVRVTTRGNNEFYKYSVTLQDKTLVVK
                     GERKSENKIKKIRSFDHLLNTSINSMFSRDYREIRIYVPNNVDINASSISSYLFISDD
                     VDSNNITYKTSSGGFSRAITENKVNRLENLNLISNSNLHLSTKSILGVKNVNIESESL
                     DISSENGDVFINNIEEYIPENVNIKEKIGRNSNYESEFYLYSDMPIAKFLDIEVPNSK
                     VRLDIPVNKYKFNCDIKSKEAIEEFNNDEEYDNDSYDYEHNEEHSNKYRNTREIKGLL
                     NKNLSNLEKEYTIKINSNSLEL"
     gene            53593..54129
                     /locus_tag="PC414_000311"
     CDS             53593..54129
                     /locus_tag="PC414_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDF2462726.1"
                     /translation="MNKIAVSFLIIATTLLSTACMDYSISAVELVDSKESAVVKKDED
                     AKEETTSKMINSKKTTKIPIEIISKDEKIVKYLQIDEESSLKDKLRLILDTLSNEYFN
                     GLDMEVEVKEKDNLVKINLIEPDKKSRVSWKDDYLNEQNIIYTINNIIKNVIQEEDNS
                     IWIEEVEIYYNGKLIELR"
     gene            54245..54952
                     /locus_tag="PC414_000312"
     CDS             54245..54952
                     /locus_tag="PC414_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HDF2462727.1"
                     /translation="MSNYILQVKGVSKKFKEQMAVDNISLAIKSNSIYGLLGPNGAGK
                     STLLKMITGILNPTSGKIMFEGHRWTRKDLSNIGSLIESPAIYENLTARENLKVHTTL
                     LGLPDSRIEEVLETVDLRDTFKKRSGQFSLGMKQRLGIAIALLNHPKLLILDEPTNGL
                     DPIGIEELRELIRSFPKQGITVILSSHILSEVEQVVDEIGIISNGVMGYQGEVSKEQN
                     LEELFMKVVAENRKRGQ"
     gene            54954..55670
                     /locus_tag="PC414_000313"
     CDS             54954..55670
                     /locus_tag="PC414_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HDF2462728.1"
                     /translation="MERFIRVEFQKMKGTFGMKLIWIVPVFCILLCGLGGGQNAAFNW
                     WYTTFLPGALTLISSLIVLKDEKSKYRGILPLPIEMKDVWTGKMIYCIICCFIASIIS
                     AIGVVFVGIIYKETISIGQNIIASILIILLFMWQIPLCMFLASKFGMFVTVLTNMFFT
                     IVGVAVFSTTEMWYMFPYSIPSRLMAVVLHILPNGLPVPEGSEFLNNAVILPGVLISI
                     VLFVLFALATRVWFNNREVK"
     gene            55672..56430
                     /locus_tag="PC414_000314"
     CDS             55672..56430
                     /locus_tag="PC414_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HDF2462729.1"
                     /translation="MRQLIRIFCSDLIKLKRNFIILMHICIALMGMGLFLWYYKVSGA
                     DNILKIVAYLQVIAIAFPLLSSVVCSMFVEQEYYAGNYKHMLTTSNPKYLTLISKYII
                     LVCLGFVATLVSVLGFKFGISKAYFTSSFYMISILILIGCNLFEYILHLFLSLRFGKG
                     TSIGVGIVEMLLSALLLTGLGSGIWQYFPCAWGVRFITIWSSFSSSKTVEYIKVESIK
                     AYQSTGLICGFVTILAFVILCIWFSKWEGKKSEE"
     gene            56565..57953
                     /locus_tag="PC414_000315"
     CDS             56565..57953
                     /locus_tag="PC414_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase"
                     /protein_id="HDF2462730.1"
                     /translation="MLGGKKSNNKKVEKLRTIFIKYLSLFFIMTISIVLLLMLSFSVL
                     LSSGVILPANYAEKQFNKYKEQIISSEKVTEDIIPSIYEYGVYTLDGNLISGTFNKKE
                     SKEVWNLMRDIEERHAYSESYIKFFKKDEVFIIKYKIVSEYSSPILRAYLPKPETLGM
                     IIFSIIFFIEIVILSKVFGKKFNIEMELLKNTTEKIEQQDLDFVVESSKIYEINNVLF
                     SMDKMKLALKDSLEKQWMLEENRKEQISALAHDIKTPLTIIHGNTDLLIEINENPELL
                     EYMEYISKGVTQIEKYINTLIDISKTENGYILNKEAISMSEFIKDVLVQIEALASTKN
                     LDVEFYKQDNLPKNITIDRELLFRAIMNIISNAIDYSPSQSKLYISVSVHNKYLKFVV
                     TDCGSGFSKADLSKATEQFYTGDLSRNSKSHYGMGLYIVNNIVQKHNGTLCIENSVKT
                     GGAMVTIEIPIV"
     gene            complement(58029..58850)
                     /locus_tag="PC414_000316"
     CDS             complement(58029..58850)
                     /locus_tag="PC414_000316"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HDF2462731.1"
                     /translation="MQKKVAAINDLSGIGKCSLSVAIPILSALKVQCCPFPTAILSSQ
                     TGYPEFTFLDFTDEMVKYSNVWKNLKVNFDSIYSGFLGSKHQIEIVANFINDYPNAFI
                     VVDPVMGDNGVMYPIFTEEMRQEIKELVKHSDLTTPNLTEACFLTGNDYTKSDYNRDE
                     LIYIAKSVSDLGPSKVVITGILEDDNILNLAYNRDNDHVFFTSVKYNNCSYSGTGDIF
                     TSILCGMLVNKHDLGVAVNTATDFIYKTINYTSQFDTDRNDGVMFENFLSDLTNI"
     gene            59086..60255
                     /locus_tag="PC414_000317"
     CDS             59086..60255
                     /locus_tag="PC414_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HDF2462732.1"
                     /translation="MGGVALKILKNKNKYIKGIMAFVFLSLATTAGVFLINDLSMKNM
                     EEKVMESANVEGDDVKDQYKLLLENLFDYRNKAILEKNENILKGLYETDKKFGLWAYE
                     NEVKKMKYLENWSSKQGVKFKDIKTRVKIKKIKEKEKDLYGIICSVSTEYKYSYENQK
                     DKENAFRIGTYHYLNVKIIDNQYVITKEWYTDPFADSLNLENIKSDDIRNYIGQQDGV
                     ELQLTDEQKKAIDYAHKYCGAAADEEHGMKFNPNYRDYNPEGGDCANFASQIMFESGR
                     FKKNSIWNYSERAGTKAWVNAQAFKNYALYSGRGSLIAKGSYEEVYKEAYNLRPGDFV
                     GYEKKGRITHVSTVTGLDSRGYPLVTCHNTDRMLVPWDLGWSDKSIRFHIIKINY"
     gene            60438..60638
                     /locus_tag="PC414_000318"
     CDS             60438..60638
                     /locus_tag="PC414_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDF2462733.1"
                     /translation="MKTGVVKWFNNEKGFGFISVEGEGDVFVHFSAITGDGYKTLEEG
                     QSVEFEVVDGAKGPQAANVVRL"
     gene            61059..61487
                     /locus_tag="PC414_000319"
     CDS             61059..61487
                     /locus_tag="PC414_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDF2462734.1"
                     /translation="MNILFFLTPKSEVAYIYEDYTIRQALEKMEYHKYSAIPIISKDG
                     KYVGTITEGDFLWTLKNDLNLDLKGLEDVPVTDINRKMDNSPVSINADIEDLVIKSLN
                     QNFIPVIDDQDTFIGIIKRRDVIGYCYEIIRGYKNLANDN"
     gene            complement(61630..62385)
                     /locus_tag="PC414_000320"
     CDS             complement(61630..62385)
                     /locus_tag="PC414_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF2462735.1"
                     /translation="MEKKVLATVGEKEITNIDIENALKSLDPYQAMQFKTEEGKKHLL
                     NDLVNQELFFLDAKEEKLDEEEIFKLEMKKIEENVLKQFAINKVLSSVNVTEDEKVKF
                     FEANKSSFSKPESATAKHILVDSDEKAKEILAQIKSEEISFEDAALKHSSCPSKDMGG
                     DLGTFGRGQMVPEFEEAVFSMAKGEVSEPVKTQFGYHIIKLEDLQESTESTFDEVKAE
                     VEKSLLYQKQNEVYGNKINALNAKYGNLVKYND"
     gene            complement(63086..63592)
                     /locus_tag="PC414_000321"
     CDS             complement(63086..63592)
                     /locus_tag="PC414_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2462736.1"
                     /translation="MNNLDKLFELASQEEIIIHYTTYIAGDLEGLYINKHGIKIISLL
                     SNLKQNSKKLTSILAEELGHHFTSLGYYVSSYNDYYTKIIIDKCENKALKWACEFLIT
                     EEDIINIINSGITCVYEMADILNVDITFFQKRLEFLSLKKQSLQLGNNKYLILTNLPY
                     FYIFDPIS"
     gene            complement(63674..63994)
                     /locus_tag="PC414_000322"
     CDS             complement(63674..63994)
                     /locus_tag="PC414_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDF2462737.1"
                     /translation="MFAKRLRELRKEFGLTQRELGEKVGVSQRVLGYYETENRFPDEH
                     ILNKLADVFNVSVDYLLGRTLVKENIDTVAAHRKNPHEELPEEAQEQLNDYIEFLLNK
                     YKKK"
     gene            64163..64360
                     /locus_tag="PC414_000323"
     CDS             64163..64360
                     /locus_tag="PC414_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2462738.1"
                     /translation="MFKNNLKYYRKCKGMTQIQLARKAGITNDYISQIERGIKNPGLL
                     MAKKISSILEQNIEEVFFIQL"
     gene            64465..64905
                     /locus_tag="PC414_000324"
     CDS             64465..64905
                     /locus_tag="PC414_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2462739.1"
                     /translation="MENKKDILFKETDKRLHNYKYLDIKIKNINLDIKRCENEYSGCG
                     AMVYTEKTSNTYNISSSVENEVLKREERLRKLKMEKEDIEIEKEKIENALTCLNDIEM
                     EFFNLFYNSKTKNNMTYISMKLHLDRTSCYNLKKKMIFKLSEIL"
     gene            65154..65597
                     /locus_tag="PC414_000325"
     CDS             65154..65597
                     /locus_tag="PC414_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2462740.1"
                     /translation="MLKYKEILETIIEILKKNFTESIFIDDESVQGSEGSCFFVSILS
                     VICTPVMLNTNNKDIVISIKYLPKPQSKSIRMYEVSDELNKLFNRNIKVTDRKLNITK
                     LEQSIKKEESIYVLNFTFTLNYLDSVYEEDVVYENMEEINLNLGE"
     gene            65602..66666
                     /locus_tag="PC414_000326"
     CDS             65602..66666
                     /locus_tag="PC414_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HDF2462741.1"
                     /translation="MAIGLPSINISFKELATTVKERSARGIIAMVLKDAKALGLNEIH
                     EKEDIPVDLSAENKEYINLALMGNVNTPNKLLVYVIEGEADIQTALDFLETKEFNYLC
                     MPKAVEADKTAIKNWIIKLRDIDKVKVKAVLGKVVGNHEGIINFTTEDVLVGEKKYSV
                     DEFTSRVAGLIAGTPLSQSVTYTKLSDVVDIPKMTKVDAESKVNKGELILIKEAGAIR
                     IARGVNSLTELTAEKGEMFQKIKIVDTLDIIHSDIRKVIIDDYIGKVTNSYDNKCLLI
                     VAIKSYLEELEKSALIESDSTVEIDFEAQKSYLKSKGVDLSYMTLQEIKEANTGSKVF
                     LKAKIKVLDAMEDIDLSIEI"
     gene            66680..67108
                     /locus_tag="PC414_000327"
     CDS             66680..67108
                     /locus_tag="PC414_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HDF2462742.1"
                     /translation="MANMEARNVMSGTWGELWLDGNKVAEVKKFQAKMEFTKEDIIIA
                     GQMGTDTKYIGYKGKGSITLYHVSSRMHKLIGEKIKRGSEPRFVAISKLNDPDSYGAE
                     RIAVKNIAFDDLTLADWEVGVKGEIEAPFTFTEYDFLDII"
     gene            67234..67680
                     /locus_tag="PC414_000328"
     CDS             67234..67680
                     /locus_tag="PC414_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDF2462743.1"
                     /translation="MSENGLSKNINIVDLLLNSDTENLERPSTIVELKRLSTIFGQEF
                     KVMCRALTISKDEEIQNTCLKIDENMKTDIDLPEMQMLTIIEGVCDLDGKLLFKNKEL
                     MDKFKAPTPKELARKLLLPGEITNLYRILQDVMGYGKNAVIEEVKN"
     gene            67707..67874
                     /locus_tag="PC414_000329"
     CDS             67707..67874
                     /locus_tag="PC414_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2462744.1"
                     /translation="MYYYWKKKGIRPSLFYAMDKGELKLIEAFFALEIEEEVEKMKHG
                     YGVCPLTGGGM"
     gene            67874..70327
                     /locus_tag="PC414_000330"
     CDS             67874..70327
                     /locus_tag="PC414_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2462745.1"
                     /translation="MGNVREEGINMYLTDNYTPKMNQIISVTDNFRRATVAVSLSTNV
                     MASSIKNSIGSASNRVNSLNSSLRKVQTTASSVSSTMTKLSSSINAVSGVIGSLNGSI
                     MILAITIAMIIDYFNKLIQKKNEFNSNIMIILIFKAKSDEVEKTKNKLLGNLKKIGGK
                     VWNIVIKAKDMTKRVISSILGKLKQVEKRPYQGSINLKDMVSSAMGRILPKLMLFKNT
                     FWSGVIAIKDMASGIISKVFPKLRLFAGKVWSGAIAVKDMASGILGSIKGKISDLTNG
                     ATIGVAVKKGVDLLGQEQNQKVVLESVMKRNTGKVNQIDVDDYYGSLVRMANDTPFDP
                     EDVVAMGTKAKMISNITGGKKEKDITQAMVDVRALNMNTSSEQDVSAAFLSAAKGNME
                     SLNTLVGENYKTFDEALEGISVKQMGLAKEMSNTIPGIISGAQTSINNGLKSIVKPFD
                     DILGQGLKKIKTFIESGLGNLAGLSEKMAGKIGNVMNGKIIIGNKYDQMQSRSVKNGK
                     EFSDSTQYRISNEAEKRKMMVENKQERFENHAATMIGNAPKAIVNAGSTLLQNIDFTA
                     LIDSLLPVVNLVNNLLDSINNKSPIAQGLISIFGTIVTTAFQLIGPVVEAVSPIITRI
                     FTFLGEYAPQINNFIETLGVIWKTVWETLGPLLETGWKIIEPILGAFFNILDKVCKIV
                     KDICKWWQTMINKIKNGSITGTVLNLVEKSKKNYKDNPYAGTKAGDSGKAYSGKKGNN
                     AFGLNYVPYNDYQTRLHEGEMVLTKQEANQYRSRKNGGNINIAKLADTIVIREEADIE
                     KITSKLVASIQLAQLGGVL"
     gene            70327..70749
                     /locus_tag="PC414_000331"
     CDS             70327..70749
                     /locus_tag="PC414_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2462746.1"
                     /translation="MEMWLRQAEDRFRFPVFPSSFSINGKAAVNSSSILKIGEIATFG
                     GVALKSISISSFFPNKDYTFCDYTGFPSPYDCVNKIEKWMKEGFILRFTITETNINME
                     VIIEGFSYEERDGTRDVYFTLDLKEYKRIKIPKVTPKQ"
     gene            71031..72560
                     /locus_tag="PC414_000332"
     CDS             71031..72560
                     /locus_tag="PC414_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDF2462747.1"
                     /translation="MIINRSKDSSSNEISFVSKDMGFLLTQSEVSYNFKDKLVEDIAK
                     QVFAENRLSVGTIAKTNVKYTKMFIGVNGYDTIMSAYTEASKKTKKKYMIEANLDKFN
                     VIEKGTVTLNVMFEEGFNIINTTFSESMENVKNKVIVVDQYGSKISEKIDNEIFKEVN
                     VIMQKVIQQQENQDVDIDSEFNGIEKSCSLKGYGDVSCITGRGVKVKDSYTKLVGLFY
                     IDTDKHTWQNGEYQIELELNFQNLMDEKSAGQDEPKEESNLGGEDYAGGKEFTAEFTA
                     YCPRKEEGGDTDCRKKKLDPSKKTCAAPMVGKYEQTYYTKEFLNKHPLLNYGDEIQVI
                     TGVSGRDGVYKVNDVGPAITIEKNGTYHIDILFGNVEEASKFGRRKGKIIIGGYSGNV
                     SDKAKIVISEAKKHLGKPYKWGGNGPSSFDCSGLMVYCFKKVNVSLPRTSNQQSKKGK
                     KVEQKNLQAGDLVFFHNPVSHVGLYIGNGEFLHAPQKGDVVKISKLSSRRDFNTARRV
                     L"
     gene            72576..72902
                     /locus_tag="PC414_000333"
     CDS             72576..72902
                     /locus_tag="PC414_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 family protein"
                     /protein_id="HDF2462748.1"
                     /translation="MANPINEFIGIIREEGKYHNQPSFFIGKIKSKLPDLKIETNNII
                     LEKEDILIDSWMIDRQLETFDTETNQEHQHEVKNPFIDNFESGDMVIMFRIGEKFAVV
                     SKLVSL"
     gene            72902..73330
                     /locus_tag="PC414_000334"
     CDS             72902..73330
                     /locus_tag="PC414_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HDF2462749.1"
                     /translation="MSTIFPFIGVPEDYILPKTEELPIFREVAWDFEKDEPILEKGDF
                     KIIEKKEALKVWIYKCIKTNRYEHEIYSLEYGTELSELIGQKYTKGLTESEASRFIKE
                     ALLINPYILEVNVKSANFNRDVLSANVKVSTIYGEVEINV"
     gene            73323..74375
                     /locus_tag="PC414_000335"
     CDS             73323..74375
                     /locus_tag="PC414_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HDF2462750.1"
                     /translation="MYSDQTYEVIKNRTLENINLDIYKGEGSFLNNMVSGNNLELSKI
                     YLELSKIHKMAFIQDTYNQFLDKRVNEFGVYRKLGTESNGEVEFIGEKGTVINNGTII
                     SYRDLLFVVIKDVTIGSEEGDNSPVQALEVGKKYNLPTNCEFKLVDNISGVTKITNTR
                     SFEGGTDIETDEELKERFYKIQRNQATSGNKAHYEEWALEVDGVYNVKVYPRWDGPGT
                     VKVLIFGKNNQAVDTETIERCQQHIDEEKPIGPTITVVTPLPIEINISAVMKLEDGYT
                     LDNVKESFLESINTYFRDIRGEIIYTKVMGILINTTGVHDLSNLLINGSTDNITINED
                     KIPSVTTVNFSEVENQ"
     gene            74372..75070
                     /locus_tag="PC414_000336"
     CDS             74372..75070
                     /locus_tag="PC414_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HDF2462751.1"
                     /translation="MKLIDKLPSFDRNYIVEEIQGAYDTELNILKEDIDDTFNQLFVD
                     TATWGLDMWEDILCIEKKELDFDTRRSNIKAKMRSRGTSTIEVIKSICEAYTKSETDI
                     KVYSDEFTFVLSFIANNCDYKTLLDCSDMIERVKPAHLLHYLEPIILDKSMVYCGGGM
                     VCSEEVKVHPYFEPIIKCSAVVNCGAGMLSREEIKVYPLSIKCIENNCKINIAIANDT
                     GVENVVVYPKSEVV"
     gene            75071..76054
                     /locus_tag="PC414_000337"
     CDS             75071..76054
                     /locus_tag="PC414_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDF2462752.1"
                     /translation="MEEKFYIILTKIGREKIANATALGELVGLTKFQVGDSNGEYYEP
                     TEEQTALKNVVWEGNINSLRIDEKNPNWIVIETILPGTVGGFMIREAAVLDNENNIIA
                     IGKYPETYKPRAEDGSIKDLVVKMILQLSNTSNVTLEVDPTLVFVTQKDIQDLDDKVD
                     KNIKEIKVKIGEELLSTEAKNLSGAINEVVEKIKNISIDDVIGGQIQTELSVLKNSYN
                     KLSEKVLDILIYLELESEIDVDEAGYWYDTLTNAKNIIAIEGLKLDLNRKCITGELGS
                     VTFKNVVLPFNANRVRYIHEMDNNFVETKSNRAYSIGQTDITLNKYSYEIR"
     gene            76071..81392
                     /locus_tag="PC414_000338"
     CDS             76071..81392
                     /locus_tag="PC414_000338"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012632.1,HMM:NF024930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2462753.1"
                     /translation="MKRTKLLQRGNFFGDKNMVVDEFDEGYDNYDFINFFTGCCNYTF
                     GLKNNNILYGCGDNSNFQLGLGEDNTTRKLFTKIPNISTNIKKVACGESHAVILTSDG
                     ELLVAGINTDGQMGLGLEKVGKTVSTFEKVPEIKGVKDIACGLQSTYLLYNDGTLYVA
                     GNNLYGQLGLGTNGASANVNTFIKVDVDNVKAVFSYNKSAFIIKNDNKCYSTGFNNQG
                     QLGLGDKNNRDLFSLVSINDVKTIACGSEHTVLMTYNNDIYGCGKEKCFGNALQSSLF
                     TKIEEVNIKTIACGNGNTMLIDNKGILKVAGNNDIYQLGIANYSENIDNSFIDLKNIV
                     AKNIFIGLSHSILIDSNNDSYCTGDNTYGQLGSFFDDMHIVEFKKMDSEKYSYSNYIN
                     LIKSEDKLTLLKEEMEIKDIELPLDIHSVRDVVFSPYCTLVILGNGDVYGLGNNRYKG
                     MGSDLPSQLNELTKLSISNVKSIVASKNISGGIFYIKNDDTCYYSGPNSNSIAGVLPS
                     NSDIFKKISIDNVKKVVINTDLSNWFSLIVTNNKQIYTSGKSSSYVNGLSNKSASQYT
                     EIGVSNVTDAYSSYNATFIVADEKKVYATGINTNYLLGVNPNDGVNINLTLINNWFYV
                     NPSGASYSTNSCTNDITKINNIIIYEYMAILCTNYGSFLTGYHGTLWTKPSDSTYRVQ
                     QQGITHAGYISNYIYNYYPTRCTQSSYSTNFAYLYYGESTSNLKNVNPDNLLISGGSS
                     YIHQYGRNYLNNQSSNNIAASNINSGPITSDKAIFLYKALLYLSSNTLYGFGNISESA
                     KELDVSDTQDGYNATNYKKVMKNIKNIFIPPYDLSRDKTRFAILTDKSLFICGYNSKG
                     THGISVNSSLNLNNKINYNKKNSSSEISSNIQEIYSHSKSTYLLTNNNMLYSVGLNDV
                     GQLGVGDEINRKVFTKINIDNIKSINVNRFTDNSKHVFAIKNDNTCYAVGLNNSGQLG
                     IGDNVNRNVFTKINVENVKYVAVYGNTSLLLTNDGLLYGAGNNGKGQLGLGDTTSRNI
                     FTRIPINGVRDVYICNDVSIIVKNDNTCYVCGFVNGYFGFTEGSISTFTKINIENVKS
                     VVTAGSEATFFITNDNVIYTTGKKERVFFSTETNDIKGIRVINNIINAKKIVVNGYTS
                     AILTNDNKLFVGGLSGYGSIANNNNTNSVEDVKDVFVTANNTLYIDNNNNLISSGRDT
                     YGISDESYRDMSVPYYKVSIKKDVDTVFSSYNTIFIKDIYGKFYSSTGDNRYNHLGIH
                     HRYDNDKNEALEGSLHSYFKTDNTSDKIVFNKKNEKLVMFNDKYIKTNNKYINYKNIF
                     KDNFKYTSIILPFEVSDIDISKTHSLAVAKDGKLYGIGSNSYKEINQTLEDIELLTLT
                     EVNISDVKKVACGDNYSYIIKTDNTLWSYGKNTEYQLGVGHNNDVRELQKVTGLPSVK
                     DISIYNSMTLVLTNEGELYAQGYNTNGLFGLGESEKDKIIRTFTKVLTNVKEIKSHND
                     DHILVIKNDNSLWITGKNKSMYKISISITDLYEFTKIPIPEHLNDILDIELSDDTIYM
                     ITKVDTSKASIEIVEKSISQVRVVVQDPNNVIEKLEMFINDELISTKTNLEINSIIFE
                     IPQNKIVLGENKILIKASSPTGDLYSSMFIFKSETGLKVKKDSILMINNKVYSIINIT
                     ENNTDLIVTLNEGLKDDMMENNPIYQLINKTKVQVKINKSDLFKDMKLVEIKKSDSSY
                     QEIYELEEANIKSAQPKIIVEKGDKWTAIKRPSMIFRYDAESNEPQA"
     gene            81409..81762
                     /locus_tag="PC414_000339"
     CDS             81409..81762
                     /locus_tag="PC414_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2462754.1"
                     /translation="MFKFDKNKIEQIKQGRKVEMQYKDISDISIGQVKQDDDITNNFI
                     ANAEIYEMLLNQSSVNEASNISSFSVRKSGGESGMVEVYVALILRGKKTIEEVPAVIR
                     EQVRIRCKELEIPVE"
     gene            82025..82285
                     /locus_tag="PC414_000340"
     CDS             82025..82285
                     /locus_tag="PC414_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2462755.1"
                     /translation="MDKLITELSSLGAIGILCALLFKNTMQEKKEDRDMYKKTVENFI
                     ELSTQQQEINKNILVQMGIMKTDVEEIKEDVTGIKGMLQNGV"
     gene            complement(82506..82961)
                     /locus_tag="PC414_000341"
     CDS             complement(82506..82961)
                     /locus_tag="PC414_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2462756.1"
                     /translation="MGLRDKFAQSFARSKTMSGPEKKANEIMGKLLLKKAILPIVLMF
                     VIIIAGAMLKINSWVTLGINLVIAVGAFFYIRNSSKKYQNFKPYVGNLISLEKKGKKE
                     YVAIIKQGKLPVKLQIAYGGEDLEHVKKNQMVQISYNPDAKIAILVNRQ"
     gene            complement(83189..83608)
                     /locus_tag="PC414_000342"
     CDS             complement(83189..83608)
                     /locus_tag="PC414_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2462757.1"
                     /translation="MDTLGERIVYLRKAKNLKQYELEEMLGCDNLSKFERNIRKPNYE
                     ILKSIAEIFNVSVDWLLNGDNLSHKSDLICDSSSNYPLNSINSNEIKLLNNFRKLNDY
                     DKAKIEGMIELKLHEYEKEKDLGKIEYNKNKDEKIDK"
     gene            83907..84116
                     /locus_tag="PC414_000343"
     CDS             83907..84116
                     /locus_tag="PC414_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2462758.1"
                     /translation="MDARKKWIPFLGVQVKQRLIELNMTQRELAKKIGVNENYLSAIL
                     NGRRTGKKYKSSIYQLLNIEYSEDD"
     gene            84148..84357
                     /locus_tag="PC414_000344"
     CDS             84148..84357
                     /locus_tag="PC414_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2462759.1"
                     /translation="MCKLDSDGVIECCRAIDDFITALSNIKSLNMERLNTLTKYSSTC
                     SILLKEGNYEGCTIVYRKMLEELKT"
     gene            complement(84574..84972)
                     /locus_tag="PC414_000345"
     CDS             complement(84574..84972)
                     /locus_tag="PC414_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2462760.1"
                     /translation="MDTLGKRIAYLRNSKKLTQRKLMDILKFENLGKYETGDRKPNCD
                     ILMSIADYFNVTTDWLLYGKEKVNVNSSVKEDKEDYLHVTNDEMTILNLYRQLNERDK
                     IKIEGILELKISEYKDLKKHSSNNNEDKMV"
BASE COUNT        34155 a         9279 c        14845 g        27080 t
ORIGIN      
        1 ttatatgaat tttaataggt aaaatatcct attgttttgt agatatatcc taattatgtc
       61 aaaaaataaa aaaattttta tttattagag tttttggaaa taataataac agatattata
      121 attaattaac agtttttgta agtatttgac aatagagagg gtgtaacaaa tgggaataat
      181 agagtcagca tcaaaattgg ctgagatggt tcatttattg gctgttgaaa aggggataac
      241 tgatatagaa gcatgggatg aagctgtgaa agaatacagc aaaatttatg aagaaagaag
      301 aaatgaatag aatgataaaa aatgaaaact tgatgataaa aaataacttt atttaaagaa
      361 attcaatttt tatagaagta ttaaaaataa tataaataaa aagatggtat ttcaaatgta
      421 agtaaaaaat cattttggga taccatcctt attatttatt ttaataatat ataagtaata
      481 aatcttactt atccatattt tttatgtaac ctcttttttc taactttttt atatgctcct
      541 tacgtatttc atcaagatta ataccatatt gttcttctag cttaaacatc atagtaacac
      601 atgtctgagc tacatccatt agttccttag cagttttctt tataatatca acttcatcta
      661 aatcattatt ttctccactt agatttctga atttacctat acattcagct aattcacctt
      721 gttcttcacc cattttgata aatgtagatt ctagtgttgg gtctaaatta ttaagctctg
      781 gtaaagatat agtttttagt ttcataaaag ctcctttcaa gttattcgta tttatactaa
      841 aacatcataa caaaaataat actaaaaaaa aactttaaat tcaacataaa aacttacata
      901 tataataaaa atcgtgaaat aaatctatat ttgtcttaat acagcctcat ataaaacttc
      961 tccaataggt ttatttatta caagagaagc tgacttatca tagactgtat ttgctttatt
     1021 aataagagca atataatcac ctttgtaata atctataaaa cttgctgctg ggtacacagc
     1081 taatgatgta cctcctataa tcaataaatc agcattactt atagcagaaa ttgttttagt
     1141 aatgacatca ctgtcaagag cttcctcata aagtactaca tctggtttta ctatagaacc
     1201 acagttgtca cagtaaggaa tattacctcc cagatttaac atagattcta agtcaaaaaa
     1261 cttaccacat ttagtacaat aatttctatg tacagaacca tgaagttcta gcacattttt
     1321 acttccagcc atttgatgaa gtccatcaat attttgagtt atgacagctt taagcttacc
     1381 catttcttcg agttttgcta aagctatatg agcattattg ggttttgcat taggatatat
     1441 taatttatcc ttataaaatt caaaaaattc ttctggatat ctaacaaaaa aagtatgtga
     1501 aactaattgt tcagcagtaa attgtttatt gagtttttgg ctaaataatc cagtagaact
     1561 tctaaagtct ggaatattag attcagttga aacacctgct ccaccaaaaa atacaatatt
     1621 attgtgatta gctattaagt cttttaagct atttgcatcc atagttacac ctccaaatta
     1681 ttatatataa attgtactac atattaggtc gtttatgttt tattaattta aataaatata
     1741 tagtttaata agttaaataa agggaatata aataaaatgt atacagagta taaatagaca
     1801 atatttacta aaataacaag ttgtcgtata attatattaa aattgtttta gtgtataaga
     1861 agggcggggg aagtacgtga agaaaaaagc agctttagca acattggcta tgttaccctt
     1921 aggtgtggta aatgcacatg ctgatggaga tataggtata gtgactataa attatttaaa
     1981 tgtaagaaat gaaccaactg ccgaaagtag catagccttt gttgctaaaa aagatgataa
     2041 agttttgatt aaagactctt ctaatggatg gtataaaata aaagctgaat ctggacaaga
     2101 aggttgggct tcatcaaaat atatagcaaa atcaaatagt gactctttaa gaacgtctac
     2161 aaataaagaa aaacaagtta tttcaaatag tttaaatatg agaaatggag caggtacaag
     2221 ttacagagta ataactgtac taaaaaaagg tcaaaaagta gaagtaatat cagagagtaa
     2281 tggatggtct aaaatcaaat atgatggaag actaggatat gtatctagct cttatttagg
     2341 agacgtttca aactcaacta ataaatcaaa aactaaacaa gtgaatacaa cttcactaaa
     2401 tgtaaggagt ggaccaaata caagttatgg tttgttaggc aagttgccaa aaggaagtaa
     2461 agtagaagta atatcagaag gtaatggatg gtccaaaata aagtataatg gaaaagatgc
     2521 ctatgtatct agtatgtact tatcggatgt aagtcaaagt aattcagaca attctagtca
     2581 aagcaatgat aaaaagaata ctgataaggt cgtaaataca gcttctttaa atgtaagaag
     2641 tggaccaggt tctacatata gtaagttggg aaaagtttat aaaggaagta aagtaactgt
     2701 actatcagaa agtagtggat gggctaagat taattttaac aataaagaag catttgtagt
     2761 aggcaattat ttatctactt cagcagatac ttcaaataat aactcaaata gtaactctga
     2821 taacagttct aatagtaatg gcaataattc ttcatcatca ggtcaagtaa atggtatgtc
     2881 aggtataagt ggggctaaaa ttgattataa atctttgagt tatactttag aatctcatat
     2941 aagtaaacaa gttgagaaag cagcatcagg aggaaatgtg atagctccaa gtaataggaa
     3001 aagcactcca agtcctgaat ttagtacttt ttcagctcaa agaacaagct catttgtaaa
     3061 tgcaagttca agtgatatag aatattattt aaatcctaaa aattttacaa atactactaa
     3121 aggtatgatg cagtttttaa agattaacag ttatagagat ggtatttcag aatcaagtct
     3181 taactcgtat cttaatggtt tatcttctag tgtatttaaa aaccagggag cagcctttat
     3241 aaatgctgcc aagaagtata atatagatgt tgtatatcta gtatctcatg ctatgtggga
     3301 aactgcttat ggtaagtcta cacttgccca aggtcaaaca ttaacatctt ataagggaca
     3361 acctcttagt aagccagtta aagtatacaa tttttttggt ataggagcaa tagataaaag
     3421 tgctaatgtt tcaggtgcag aagcagcgta ttccaatggc tggactagtg tagaagctac
     3481 aatagatggc tctgcaaagt ggatatcaca aaattacgta aatagttcta agtacaatca
     3541 gaatactatc tacaagatga agtggaatta tgattataca tggcatcagt atgcaactga
     3601 tgtgaactgg gctaatggaa tttctggaat tatggaaaat ttaattggtc tttatggagg
     3661 aggaagtagt ttggtttttg aagttcctca atacaagtaa tacataaata aaaaatatag
     3721 actatgggta gttttcattt tatttaaatt atctatagtc tttttgtata taatttataa
     3781 tctgtaaatt taatgctaac aaaactttta tgttataata ttaattctaa gtaaatataa
     3841 gtgagtaata ggtagaaata gataaaaagt ggtaataata tatataaaga taattttgag
     3901 aaaggaggat atatatggaa agtattaagg aatatttgga taagttagaa attaataata
     3961 gtggtcttgg aaagcaactt aaagaagttt atataaatag agtagtttat tttaaggaag
     4021 ataagatagt ttatttttat ctaacttcta aagatattgt ttctcatgaa cttttagata
     4081 aatttaaaga agaattgatg tataaactcg attattttaa agatatgagg atgaagataa
     4141 gatttactgg tcttgaaaga aaatccaata aagatgttat aaaaaaatat tggaataata
     4201 tattgtatat attaaagtat ctttgtccat ctattgcagg gtggtataaa caagtagaat
     4261 ttctttgttt agaagaagaa ttaaaaataa aacttccaaa aggtatattt tatgagcgat
     4321 taatgaaaaa aaatgtaact tatgtactta aaaccgtgct tagcgaagag ttgggattag
     4381 atttaaatat aacaatagaa aaagctgttg atgaaaaggt aaataaagaa agactcataa
     4441 gaatcaatga tagagaaatg gaagaaaaaa ttagggcttt agaaattgga aaagttaata
     4501 actgtgaaga aaatgaagag gaaagttatg tcattaagtc tgatgtagat gaaaatttaa
     4561 tctatggaga taatgcaaat gctatgattg aaaatatagt tgaattaaat gcatcttctg
     4621 gaacagttgc agttgttgga gatatatttg atgttgacac taaagaactt aaaaacggaa
     4681 aaatacttat gatagcttca attacagatt atactagctc aataagctgt aaactttttt
     4741 taacagatac aaataaggat ggagtacttg aaaatgttaa aaaaggagca tacttaaaaa
     4801 tcaagggtga catagtttat gatacatatc aaagagaaat tagtatgatg attagtggga
     4861 taagaaaaga aactagaata gagagagaag ataaatcaga ggaaaaaaga gtagaactcc
     4921 atgcacatac acaaatgtct tctatggatg ctatttgttc tgttaaaaag cttgtagagc
     4981 gtgcagccaa atggggtcat tctgctattg ctataacaga ccatggtgtt gtacaaggat
     5041 ttccagatgc tatgaatgca ggtaagtcaa ataatataaa aatactttat ggagtagaag
     5101 ggtacttggt tgaagatgat tctctcatta tagaggatgc aaatgataaa gaactttcgc
     5161 agacatttgt tgtatttgac atagagacaa ctggtttttc taatacaaat gataaaataa
     5221 ctgagattgg tgctgtaaaa atagaaaact ttaagattgt agatagattt agtgagttaa
     5281 taaatcctga aaaagatatt tcttataaga tacaggaatt gacaggaata actaatgaat
     5341 taattaaaga caagccaacc attgaagaag ttcttcctaa gtttatggaa tttgttggag
     5401 atagtgtatt agtagcacat aatgcagagt ttgatactgg atttatatcg caaaaatgta
     5461 gggaacaagg cttgttatat aataacaaaa aagtagatac acttatgtta gcaagagtaa
     5521 tgttgccaaa cttaaaaaga catagattaa atgtcgttgc aaaagaactt ggtattcctc
     5581 ttttaaatca ccatagagca gtggatgatg ctgaagctac agcacttata tttaataagt
     5641 ttttgcagat gcttacagaa aaaggtgcaa aaactctaag tgatgtaaac aatatacttg
     5701 gaaagattga ttacacaaag cttagcacaa atcatataac cttgatagca aaaaattcta
     5761 taggtataaa aaatttatat aaaataattt cagatgcaca tgtaaatcat ttttttagag
     5821 caccaagaat ccttagaagt gtattaaatg aatataaaga aggattaata attggttcgg
     5881 cttgtgaagc tggggtggtt tttcaagctg taaagaaaaa cgtgtctgat gaggaaatga
     5941 aaaaaataat agatttatac gattatattg aagtaatgcc tatagataat aatagattta
     6001 tgattgataa aggagaagta aaggacgaag aagagttaag ggagttgaat aggaaattaa
     6061 ttgatacagc caaaaaattt gacaaaattc cagtagctac tggggatgtt cattttttag
     6121 acaaacatga agctgtctta agaaaagttc ttaaatattc tcaaggtttt aaagtagatg
     6181 aagaagaaac ttatttacat tttagaacaa cagatgaaat gttggaagag tttagttatc
     6241 ttggagaaga attggcatat gaagttgtag ttgaaaatag taatctgatt gccgatatgg
     6301 ttgaagatat aaaacctata ccagatgata cttatcctcc aattatagag ggctcagatg
     6361 tggagttaag agaaatgtgt tacaaaaaag ccaaacgaat ctatggagac ccaataccag
     6421 aaatagttca aaaaagactg gatagagaat taaattcaat aataagcaat gggtatgcag
     6481 ttatgtatat tatagctcaa aaactggttg caaaatctct ttcagatgga tatttagttg
     6541 gttcaagagg ctcagttggc tcatcttttg cagctactat gagtgatata acagaagtta
     6601 atcctcttcc agcacattat atttgtgaaa acgaagactg taaatattca ttcttttatg
     6661 aaataggtga atggggctca ggtgttgatt taccagacaa ggattgtcca aagtgtggaa
     6721 gaaaacttgc taaaaacggt catgatattc catttgaggt tttcttaggt tttgaaggtg
     6781 ataaagaacc agatatagac ttaaatttct caggaaatta tcagccaaca attcataaat
     6841 acacagaaga actatttgga gaggggtatg tatatagggc tgggactata ggtactgtag
     6901 cagaaaaaac agcttttgga tacgcaagaa aatatgtgga agaaaataat ataagtgtac
     6961 caaatgcaga agtacttagg ctttcaaatg gatgtactgg agtaaaaaga acctcaggac
     7021 aacatcctgg tggagttatg gttataccag actataaaga tgtatacgat tttacaccta
     7081 tacagtatcc agcaaatgat acttcttgtg gtgttataac tactcacttt gattatcatt
     7141 caataagtgg tagaatactt aaacttgata tacttggaca cgacggtcct accattataa
     7201 ggatgcttga agatatgact ggaataaata taacagaaat tcctttagat gataaagaga
     7261 ctatgtctct atttacatct acagaagcat taggtgttac accagaagaa ataaactgtc
     7321 ctataggttg ccttgctgta ccagagtttg gaactaagtt tgtacgacaa atgttacttg
     7381 acacaaaacc tactacattt gcagaacttg tacgtatatc tggtctctct catgggacag
     7441 atgtttgggt aaataatgca caagacttag ttagagaaga tatagttgga ttaaaagatg
     7501 ttatatctac acgagatgac ataatgaact atttaatatt taaaggatta cctcccaaaa
     7561 tgagctttac aataatggag agtgtaagaa aaggtaaagg tttaaaacca gaacacattg
     7621 aagaaatgaa aaaaaataat gttccagaat ggtatatagg ctcatgtaaa aagattaagt
     7681 acatgttccc aaaagcacat gctgttgctt atgtaatgac atcatttaga attgcatatt
     7741 gtaaagttca ctatccagaa gcattttatg cgacttattt tacgacaaaa gttgaggatt
     7801 ttgatgcaga tttaattgtt aaaggattag atgctataaa atcaaaaata aatgaaattg
     7861 agtcactggg aaatgatgca accacaaaag aaaaaggtat gttgaccgtt ttagaagttg
     7921 ctcttgaaat gtatgctaga ggaataaaat tattaccagt tgatatatat aaatcagatg
     7981 caactgagtt tatagttgta ggagaaaaaa cattacttcc accaatggca gcaatacaag
     8041 gtcttggagg aaatgcggct ataaacatcc aaaatgaaag aaaaaatggt gaatttatat
     8101 caaaagaaga tttgagaaag agaacaaaaa tatcaaaaac ggtaatagaa actcttacta
     8161 atcatggttc acttgagaat atgagtgaga gaaatcaatt gtcattgttt taagttagcc
     8221 caaaaagttg caaataaaag aattttatga tatcatagta tagaaaatag tatactttgc
     8281 agaaactaag agtgagacag ctgttctcac tcttttattt taaatatatt attaaaaagc
     8341 tggtttattt ttattaaata tattttattt aagattatat aaactggtca tttacaattg
     8401 aataggagga actttaatga aaaaaaattt agaagctaca attgaggaga ttgtaactaa
     8461 aataacggat gaacatggat ttgaaatggt tgatgttgaa tatgtaaaag aagcaggaga
     8521 atactactta agagtataca tagataaaga agaaggaata tctttaaatg aatgtgaatt
     8581 agttagtaga gaattaagtc caatactaga tgaaaaagac cctataaaag aaaattactt
     8641 tttagaggta tcttctccag ggcttgatag agcattaaaa aaagacagag attttgttag
     8701 atatcaaggt agagatgtag atttaaaatt atataaacca ttaaatggat gtaagcaatt
     8761 tgaaggtgaa cttgtaggat taacagaaga taataatata aaaatcattg caaatggtaa
     8821 ggaaatggaa tttaatagaa aagatgttgc tattgtcaga ttagcaataa agttttagtg
     8881 gtatattaaa tagggaggat ataaaaaatg aatcatgaat ttatggaagc attagatgaa
     8941 ttagtagttg acagaggtat agataaagaa atacttatag atactataga acaagctctt
     9001 ttaactgcat acaagaaaaa ctttggttca gcacaaaatg ttagagttga atttgataga
     9061 gacaagggag atataaaagt attttctcaa agagttgttg ttgatgaatc agatttatat
     9121 gatactttct tagaaataga gttagcagat gctagagaga taaaccctaa ttatgaatta
     9181 ggtgatatta tagaacatga agttacacct aaaaactttg gaagaattgc agctcaaaca
     9241 gcaaagcaag tagttgttca aagaataagg gaagctgaga gagaaatagt gtataatgaa
     9301 tttatagaaa aagaaaatga aatagttaca ggagaggttg ctagagcaaa taaaaatgtt
     9361 gtttatgtaa atcttggaag aatagaaggt ataatgactc aaagtgaaca aataccattt
     9421 gagaattata aagcaggtca aaaaataaaa ttctatatac ttgaagttaa aaagactaac
     9481 aaaggacctc aaatagttgt atcaagaagt catccgggtc tagtaaagag attgtttgaa
     9541 gaagaagttc ctgaaatata tgaaggaata gttcaaataa aatctgtagc aagagaagca
     9601 gggtcaagaa ctaaaatggc agtaagatct atagatgaaa aaatagaccc aattggagct
     9661 tgtgttggac caaaaggact tagagtaaaa aatatagtgg atgaacttgg cgatgagaaa
     9721 atagatataa taaaatatag tgaagaccca gcagagttta tatcagcagc acttagccca
     9781 tcaaaggttg ttagagtaga tatagatgaa gatgaaaagt ctgccttagt aatcgttcca
     9841 gattatcagt tatcgcttgc tataggtaaa gaaggtcaaa atgcaagact agcagcaaaa
     9901 ttgactaact ggaaaataga tattaagagc gaatctcaag cagaacaaga aatgttaaat
     9961 cttgaaaatt ccaaagaaat agaagctgag gatgttgaag ctgaatttat cgaccaagaa
    10021 gagttagata cttcagagga taattttagt gaagaagagt aaggagggat gattttgaaa
    10081 aaagtaaaaa aagtgcctca aagaaaatgc atagcatgtc aagatagaga ctctaaaaaa
    10141 gagcttataa gaatagttaa aaataaagaa ggaaaaatat ttttagaccc aacaggaaga
    10201 gccaatggaa gaggagcata tgtatgtaaa agctctgaat gtctaaaaaa agctataaag
    10261 agtaaagcat taaataaagc ttttaaaata gatgttcctg atgaagtcta tgataatcta
    10321 cttatggagt tagaagaata tgaaaaataa taaagaaaaa gtactttcgt tcttagggct
    10381 cgcaactcgt gcagggaaaa tagtgtctgg tgatgattca actttgcttg accttaaaaa
    10441 aggaaaagta aatttgattt taatcgcaga agatgcatca aataatacta aaaaactttt
    10501 taaagataaa tcaactttta gaaatattcc atatctattt ttttcaacaa aagaggaaat
    10561 aggatttgca ataggaaaat ctccaagagc agttgtagga ataaaagatg aaaatttttc
    10621 taaaaagata attgaactaa tagaaattta aaataaaata gggggtgatc ttgtgtcaaa
    10681 aacaagagta taccaaatag cagaagaatt aaatatatca aatgaagagc ttataaataa
    10741 gttagctgaa ttagatataa atgtaactga caaagattca gtattagaag gtgaagagtt
    10801 agaattagca cttgaaatgt taggtgaaga tttatctcaa gaaaatggaa atgttataga
    10861 aatagatgga aagttgacag tacaagtatt agcaacgaaa ttagataaat ctccatcaga
    10921 aataattatg aagcttatga aaatgggaac tatggcaaca ataaatcaag agataagttt
    10981 tgagattgct gctttggctg ccaaagacta tggatttgaa ttaacagtag ctgaaagtga
    11041 tgatacagaa gctttagaaa tagaagcttt aatggaaatt gaagaagata aagaagagga
    11101 tttaaaacct agaccaccag ttgttacagt tatgggacat gttgaccatg gtaagacatc
    11161 tttactagat gcaataagaa aaactgatgt gatttcaggt gaagctggag gaataactca
    11221 gcatataggt gcttcagaag ttaaaatcaa tggacacaaa atagtatttt tagatactcc
    11281 aggacatgag gctttcacat caatgagagc aagaggagca caagttacag atatagcaat
    11341 attagtagtt gcagcagatg atggtataat gcctcaaaca gttgaagcta taaaccatgc
    11401 taaagcagca ggtgtacctt taatagttgc tattaataaa atagataaac caggtgcaaa
    11461 cccagataag gttaaacagg aattagcaga ccaaggatta ttagttgaag actggggtgg
    11521 agaagtaata gcagttccag tatcagctaa gaaaaaagaa ggaatagata cacttttaga
    11581 aatggtatta ttggttgctg aaatggaaga gttaagagct aatccaaata aaagagcagt
    11641 aggaactgtt atagaagctg agcttgataa aggaagaggg ccagttgcaa cagtacttgt
    11701 tcaaggtggt actttaacag taggtgaccc aatcgttgca ggtgttgcat gtggtaaagt
    11761 tagagcaatg ataaatgcaa aaggtaagag agtaaaaaca gcaggaccat ctacagcagt
    11821 cgagatatta ggtctatcag aagttccaca aggaggagac caatttgtcg aagttccaac
    11881 tgataagatt gctagaagtg tagcagcaag aagacaacaa atagttagag atgaaatgtt
    11941 aaaatcaact caaagattat cactagatgc tctatttagt caaatgagtg aaggttctat
    12001 aaaagacctt aatatagtta taaaagcaga tgtacaaggt tcagtacagg cagttaaaca
    12061 atctttagaa aaattatcaa atgaagaagt tcaagttaaa gtaatacatg gtggtgttgg
    12121 agctgtaaca gaatcagaca tattacttgc agcagcttct aacgcaataa taataggatt
    12181 caatgtaaga ccagttccag gtgcagaatc tctaggagaa aaagaaaatg ttgatataag
    12241 aacatatact ataatataca aggcaataga agatatacaa gcggctatga ctggtatgtt
    12301 agaccctgaa tatgttgatg aagaaacagg aaaagctgaa ataagagaaa tatataaaat
    12361 atctggtgtt ggaacagtag ctggatgtta tgtgactaat ggtaagatat ttagaaattg
    12421 taaagtaaga ttagttagag atagcataat aatacatgaa ggtgagcttg cagcacttaa
    12481 gagatttaaa gatgatgtaa aagaagttaa ttctggatac gaatgtggta tgagttttgt
    12541 aaattataac gacataaaag aaggcgatat agtagaagct tatataacta aagaagtaga
    12601 aagaaaatta taaattcgta tcaaagaaaa tcagttagta aagaaggtcg atggtatggc
    12661 atcatataat agaacaagaa ggatagcaga agaaataaga aaagtcgtaa gcactatgct
    12721 tataaatgga gtaaaagacc ctagaataac atctatggtg agtgtaactg atgtagaagt
    12781 tacaaatgac ctaagatatg cttatgtata tgtaagcatt ctaggtggag atgaagaatc
    12841 cacactaaca ggtcttaaaa gtgctggagg atatataaga agagaagtag gaaaaaatat
    12901 aaaacttaga tatattcctg aaatagtatt taaacttgat gattcaatag aaaaaggaat
    12961 gtatatggat agccttataa aaagagttaa tgaaaaaaat gctcaacaaa atgaggatga
    13021 aaattatgat gaataatatg gatagtatta taaactatat ttatgagggt gactatttta
    13081 tagttacctc tcatatcagt ccagatggag acaatattgg ttctacactt tcaatgtatt
    13141 atacattgaa gaatttagga aaaaatgtat attatgtact tgatgatgaa gctccattaa
    13201 atttaaggtt tttggttgaa gatgtaaaaa tctataagtc taatgaattt aatatgaaga
    13261 actattctct aattgcactt gattgtgggg ataagatgag aatatgtgtg agtgatgaaa
    13321 tcaaaaataa tgcatcaaaa ttaatctgta ttgaccatca tgctagtaat gattcatatg
    13381 gagatttaaa ttatgtagat attgatgcat cttctacatg tgaattagtt tataatttat
    13441 tagttagatt tcagcaaata aaagatatta atattataaa tgaagatata gcaacatgtt
    13501 tatatacagg tcttgtaact gatactggaa acttttcgta ttcaaatgta catgcaagta
    13561 gctttgaaat ggcaaaaaat ttacttgttt taggtgcaca aaaaaatact ataatacaaa
    13621 atatatatca aagcaattct tctggttatt acaaattatt aggagaagct ctaaagggtt
    13681 tggagatatt tgattcaaag gtatctagta tagttttaac tcaagacatg atgaatagaa
    13741 ataatattag ctttaatgat gttgatggaa taacaacata tacaagagac ataaaaggta
    13801 tagaagttgg aattttattt aaagagaaaa aacaaaatga aattaaagtg agttttaggt
    13861 caaaaaatta tgttgatgta agtgaaatag ccaagttatt tggtggtggt ggtcatgtaa
    13921 gggctgctgg atgtactatt agagatagta ttgataatgc aaagaaaatg gtactagagg
    13981 cagtgttaaa atcaatttaa ggtgacagta aaatgaataa gattataagt atattgaaac
    14041 ccacaggtat gacatctcat gatgttgtaa gtagagtgag aaagatttta aatataaaaa
    14101 aagttggtca tacaggtact cttgacccag atgcttcagg tgttttacca atctgtatag
    14161 ggaaagctac aaaagtatgt gaagttattt taaacaaaga taaatcgtat atatgtgagc
    14221 ttactttagg tataagtaca gatacttatg atgcttcagg tgaaatttta aaaaaagttg
    14281 atgattttaa atttagtaat gaagacatag aaagagcttt tgacacacaa agaggagaga
    14341 taaatcaact tcctccaatt tattcagctt taaaggtaaa tggaaagaga atgtgtgatt
    14401 tagtaagaag tggtagacaa tctgaaatta ctttaaaaac aagaagagta aatataaaag
    14461 acataaaaat actaagtata aaaggtaata aagtaatgtt ttatgttgag tgttctaaag
    14521 ggacttatgt gagaagtatt tgccatgata taggagaata tctaggttgt ggagctcata
    14581 tgtcgttttt gaatagaaca tcttctggta aatttgactt agataattca ataacattag
    14641 aagaattaga attattttat gaaaataaaa ctttagacaa atacttatat gatatagatt
    14701 atgtccttga ttcgtttaat tatgttgtat taaaccctaa tgctataaaa tattatagta
    14761 atggtggctc aatagatgat aaaaggtttt taaagaataa tttcgataaa gatgatgaat
    14821 ttgtaagagt atacagtaca gataatttct taggattagg aaagttatca aaacacaata
    14881 atacaataag tgttaaaagt gataagatgt tcatataacc aatgtattta aaaaaacaac
    14941 aaaagagata gtactataat aacttctata gtctatctct tttacttatg ctataatagt
    15001 ttgtgaataa gttaagagta aattatattt tagcggggaa catttattat ggtgattata
    15061 aaatctataa aagaaatagc aaacataaaa gaaagtgtca ttaccatagg aaattttgat
    15121 ggtgtacaca aaggacatca agttcttata ggaaaaacag ttgaacatgc aaaaaaagaa
    15181 aatatacaga gtattgtatt tacatttgca aatcatcctg taaattactt tagaccaaac
    15241 tcaacaaaaa atattataag caatgatgat aaaataaaat tattagaaga tttgggaata
    15301 gatatagttg taatcatacc atttgatgag tatatgacta aaatacctgc aaaggatttc
    15361 gtagatgaga tactagtaaa aaaattaaaa gctaaaaaga tagtaattgg acatgacttt
    15421 acatttgcaa gggcaaaaga aggcaatgtt aatctattaa aaagcttaga acatgaattt
    15481 ggttttgagg tagaagtaat aaaacccatc aaaatcaatg atgtaagagt gagtagtact
    15541 tatattagaa gtttagtatc ccagggtgat atggctaatg tcaaggaata tttgggtaga
    15601 aattacaagt tagaaggttg tgttatacat tctaaacacc ttggaagaac tataggattc
    15661 ccaacggcaa atattgacct taagaataat atgctcgttc caaagagagg tatttatgct
    15721 tctattgtac atattggaga tgagacttat tttggtgcta caaatgtagg ctataaccca
    15781 acagttaatg gaaaaacttt gtctattgaa acaaatattt tagagtttga tagagatatt
    15841 tatggggaaa atataattgt tgaatttttg gaaagaataa gagatgaaag aaagtttaat
    15901 tcaatagatg acttaaaaaa tcaattgtat aatgatacaa attatgtgta tgaaaactat
    15961 gtttgcaaaa acaagtaatg tatgataaaa tgattaagga aaataaccat tactcggcat
    16021 ttgaatactc caatcaatgc ttagtaatag gcgtttctaa aaaaatacgg aggtaatcta
    16081 aatgataaac aaatcagaaa taataaaaga atatggaaga gctgaagggg atactggttc
    16141 tccagaagta caaatagctt tattaacagc tagaataaac gagttaaatg gacatttaaa
    16201 aactcataag aaagaccacc attcaagaag aggactatta aaaatggttg gtagaagaag
    16261 aaacttatta gcttacttaa aggaaaaaga tcttgaagga tatagagcat taatagctaa
    16321 attaggatta agaaagtaat ttcattcttt ataaaaaagc aggtatagtg tatacctgct
    16381 tttttatagt taattcatta taaagtaaaa tagttaataa taaaagtagt ttatatgtta
    16441 taaaaaatta taaaatgaat aatattgggt aagaatatta aaatagtata caaatacata
    16501 atcaatttaa aaggaaagac aggataaata tagaaatttt ataacaggat aattagttgg
    16561 aataaggagg atattatttt gaaaataaag ttagtatgcg atagtttgtg cgatattcca
    16621 gatgaaatat cggaaaaaga ttatatagag atagttccac ttacagttat ttttaatgat
    16681 agggaatata tagaaggagt agatataaag aaagaagagt tttataaaaa agtaaaagaa
    16741 atcaagcaaa tacctaaaac ttcacaagct acatatatgg agtttaaaga agtgtttgat
    16801 aagttcataa cagaaggtta tcatataatc tgtatgacag gggctgcaaa cgcatcagga
    16861 acttttcaaa gtgctatgat agctaaaaat gatgtaaatg aaaatgaaaa aatacatata
    16921 tttgatacta gaaatttatc tctaggtagt ggacaatatg ttataaaggc atgtgaactt
    16981 cttgaagaag gactgggatt tgaagaaatt atagatgaat tagaaaatac tcgcagtagt
    17041 gtaagattgc tttttgcacc atatacactt gattttttaa agcagagtgg aagagttcca
    17101 gttgcaactg ctttgatagg aaatatgtta aatataaagc ctatattttt ctttgataat
    17161 ggggaagcta agttggtaaa taaggttaga ggaataaaga atatagcaag taaattggtc
    17221 gatataattt tagaaatgaa tgaagggtct ttagaaggaa aaatcgtcac aataggttgt
    17281 ggagataatt tacacgattg tgaaatatta aaagatgaag taaataaaag aataaaagca
    17341 agaagagtat tatttactcg aggaggagtt tctatttgct ctcatacagg accagatata
    17401 ttggctataa gttgttctaa ttagtagtaa tataaggttt ttgcatatat atgtgaagat
    17461 aattttatat tgttaattta tagtattttt gataatatag taaatagagt gatatacgtg
    17521 ttttttaata agaggaggac aagcattaat gtttgaacat aaaatattta aaatggattt
    17581 tgcaggaaga gaactttctg tagaaatagg aaagatttgt gaaatggcta gtggtagttg
    17641 tatagttcga tacagtgata gtatggtaat ggtaaataca actaaatcag ccaaacctag
    17701 agacggaata gacttttttc ctttaagtgt ggattatgaa gaaaaattat actctgtagg
    17761 taagatacct ggaggttttt taaagagaga aggcaagcct tcagagaaag ctatacttac
    17821 atcaagattg atagatagac caataagacc tttattccca aaaggattta gaaatgatgt
    17881 tcaggtagtt gcaacagttc tatcagtaga ccaagattgt acaccagata tagtagcaat
    17941 gataggttct tcaatagccc tttctatatc agacatacca tttaatggac caacaggttc
    18001 agtatgtgtt ggccttgtag atggtgcatt tgttgtaaat ccaaacgcag agcaaagaga
    18061 aaaaagttca atgcatctag ttgtttcagg tacaaaggaa gcaataatga tggttgaagc
    18121 cggagctgat gaagttccag atgaggtaat gcttgatgct attttatttg ctcaccaaga
    18181 aataaaaaaa atagttgagt ttatagaagg tatagttgca gaagttggaa aagaaaaaat
    18241 gcctgttgaa ttataccatg caggagaaga aataactcaa ttggttcgcg aatttgcaac
    18301 tgataagatg aaaaaagcag ttcagacttt tgaaaagtta gagagaatgg aaaatatgga
    18361 tagagttaaa gaagaaactt tagctcactt tgaagaaact ttagaagatt ttgaagattt
    18421 tgttggagat atagaagaag tattacaaga tataataaaa gaagaagtaa gaaaacttat
    18481 agtacatgaa aatgtaagac cagataatag aaaacttgaa gaaataagac ctatatggtg
    18541 tgaaactggc atgataccta gagctcatgg ttcagccata tttactagag gacaaactca
    18601 agtacttaat gttgctactt taggtgcttt aggagatgtt caaaaactag atgggttaga
    18661 tgaagaagaa aacaagagat atatgcatca ttataatttc cctgcatata gtgttggaga
    18721 agctagacca tcaagaggtc caggaagaag agaaatagga catggtgctt tagcagaaag
    18781 agctttatta ccagtaatac catctcaaga agaatttcct tatgctatta gattagtttc
    18841 tgaagtatta agttcaaatg gttctacatc tcaagcaagt gtatgtggtt caacattatc
    18901 tttacttgat gcaggtgtac ctataaaaga tatggttgca ggtatagcaa tgggacttat
    18961 aaagcatgat ggtaaggttg cagtgctttc agatatacaa ggtatggaag accatttagg
    19021 cgatatggac tttaaagtag ctggaacaga gtatggtata acagcaatac aaatggatat
    19081 aaaaattgat ggtatagata aagaaattct acaacgtgct ttaaaacaag ctaaagaggg
    19141 tagaatccat atactaggag agatgagaaa aactatatct cagccaaaac cagaactttc
    19201 accatatgca cctaagattg ttaaaatgca aataaatcct gacaagataa aagatgtaat
    19261 aggacctggt ggaaagataa taactaagat aatagacgaa actggagtta agatagatat
    19321 tgaacaaact ggggaagtat ttatatcagg tattgagatt gatatgatta agaaagctca
    19381 agaacttata aataatatag tagttgaacc agaagtagga aaaacttata aaggtaaggt
    19441 aagtagaata atgaactttg gagcatttgt tgagatactt ccaggaaaag aaggattact
    19501 tcatatatct catatagctc atgaaagagt tgccaaagtt gaagatgtat tgaatatagg
    19561 ggatgaagtt gaagttaaag ttacagagat tgatgaaaaa ggaagagtta atttatcaag
    19621 aaaagttctt ttaccaaaac cagaacataa aaataaataa cttattatat aattaaaagc
    19681 aaagtcttag gattttgctt ttttaaatac aaaaaagtag ataattttaa aatgatagtt
    19741 atggatggct tatagttctt tttctgtcag tctttactct tataaagaag ctatttaaaa
    19801 gctgattaat agaaagcttt attgatatat atagaatcac ttaagttata tttagtgcat
    19861 aaacagagct tatttttaat agtattaaat aagaaacaat ttgtacataa ttagggggag
    19921 ttttaatgat aatgatggta cttaataaga gtaagctcaa aaaaatcata atactaattt
    19981 tagtagtgtt attagtatta tcaggagggg taatggtatt aaagttattt aacaacaagc
    20041 cagtatttaa tttatttaat ggtgtagatt taccatatga aagaggtaca aaggataaaa
    20101 gtggatatgt tgcattaacc tgtaatatag accttggatg ggaaacagag tacgtagaat
    20161 ccattttaga aacacttaaa aaagaaaatg taaagattac atttaatgta actggaaaat
    20221 gggcagaaaa gaataaggat gaattattaa agataaaaaa acaaggacat gaaataggta
    20281 atcatggata taaacattta gattattcta cattatcata tgaagacaat tatgagcaaa
    20341 tagagacctc taaaaagata attgaagaaa ttataggtga aaaaactaaa ttttttcaag
    20401 caccagcagg ttcctttggt ccagaaacag taaaggctgc taaagcatta gggtatactt
    20461 ctatcaaatg ggatgccgat actatagatt ggaaatacaa agaccaacca gaggtaatca
    20521 ttgatagaat gaagaaaaaa gacattaaag atagtagtat aatcttaatg catccaacca
    20581 acgcaacgac aaaatgtata gatgatatta tagctattgt aagagaaaaa ggactaaaag
    20641 caggaaaact tagtgatgta tttaagtaga caaagtataa atatttggtt aagattgtat
    20701 aagttgtagt tgaattaatt aaaagactga cttataatgt aatacatata aaaaataaat
    20761 ataattaaaa catagtgttt tcaataatcc aatatgaaac aaagaataaa ttgttaggag
    20821 aatgtaatgt ataagacaaa aatactggaa aatgggctta caataattgg tgaagaaata
    20881 ccatatctaa agtcaataac attaggtatt tggataaatg caggctctag gatagaagaa
    20941 gcacaggtga gtggaacttc tcattttata gagcatatga tgtttaaagg aaccaaaaac
    21001 agaacatcaa aagagattgc tagttcaata gataatttgg gtggtcaaat aaatgcattt
    21061 acaagtaaag aatgtacatg ttattatgta aaactaatag atgagcatat agatacagga
    21121 attgatgtat tgagtgatat gatactaaat tctaaatttg ataaaaatga tatagataag
    21181 gaaagactta taatattgga agaacttaag atgtatgaag actctccaga tgacttatct
    21241 tatgatttat tagtggaaaa tatatatgct aatgatggat taggaatgaa tataattggg
    21301 acaaaagaat ctctatacaa tataactaga gaatccatgt tagaatactt aaataagtat
    21361 tatattccaa ataacgctgt tatttctata gctggaaact ttaactttga tgatatggtg
    21421 gagaaaatca aatcaaaatt tggacactgg gaaaagaaaa atttgagtat agacataagt
    21481 gaggctaagt ttaatccttg ctttatatca aaaaataaag atactgaaca ggtgaactta
    21541 gctatgtgcc taaaaggtat tccttttgaa aatgatgaag aagtctattc aatggcagtg
    21601 gtaaataata ttttcggtgg aagtataagc tcaaggcttt ttcagaaaat aagggaagaa
    21661 aaaggtttag tatattcaat atactcatca cagacattat ataggaaatg tggtgaatta
    21721 ggtatatttg caagcatgag tacagaaaat ttacaagatg tatataattt aataaagaaa
    21781 gaaattgaaa atataagaga aaactatctt acagaaaaag aaatatctga aagcaaggaa
    21841 cagttaaaag gaaattatat attggattta gaaagtacta gcagtagaat gatgtctact
    21901 ggtaaatcta tgttacttag taaaaaagta aaaactacag atgaaatact tgaatgtata
    21961 aatagtgtta atattaattc aataaagaag gttgttgata aggtattcaa tatcgaaaat
    22021 attggtacat gtatagttgg aagagatgta gaaaagattc tacacttgga ttaatattga
    22081 aaaaagtaaa tctctggagg tataaaatat gaatttatct gaaatagcag gtaaggaaat
    22141 agttaacttg gtcacaggag aaagattggg ggtcattgga gaatgtgacc ttatactaga
    22201 tgaaactaca ggcggtatat tagcgcttct aattccaaaa gaaaaaggat tttttgccag
    22261 aagaaaagat aagtcatttt tagaagtacc atggagaaat gtaaaaaaaa taggtaatga
    22321 catgataatt atagaatatg agggatatat ttaggaggaa aaaatgagta ttttagtgca
    22381 aaagtttggt gggacatcag tagaatcata tgaaaaaatg aatgaagtat gtaagatagt
    22441 aaaagcatac aaaaaaaatg atgaagaatt acaattagta ttagtagttt cagcaatggg
    22501 aagaaaagga gctccatatg ctacagatac acttataaat ttgtgtagtg cagtaaatga
    22561 tgagccaagt aaaagagaac ttgatttaat aatgtcttgt ggagaaataa tatcaggaac
    22621 aattcttgct aatttattga atgcacaggg gatagattct gttttcttta ctggtcagca
    22681 agcaggtata attacaagtg atgaatattc taatgcaaag ataaagtata taaatcctaa
    22741 aaaggtaaaa agagctttag atgatggtaa ggttgtagtt atagctgggt tccagggtgt
    22801 aacagatgat ggagaaataa ctactcttgg aagaggcgga agcgatacat cagcagttgc
    22861 tattggtaaa gctttagaat gtgaaacagt tgaaatttac actgatgtgg atggtataat
    22921 gactgcagac cctagagtag aaccaaacgc taaagtattg agttttatag actatgagga
    22981 agtattccaa atggctgaca aaggtgcaaa agtaatacat ccaagagcag tacagttggc
    23041 taaaagtgga aatataactt tagccataaa gaatacaatg aatccaactt ttgaaggtac
    23101 aaaaataggt tctttatgta gacacttaga agacaatata gaatatgaac aagaaagaga
    23161 ttttaaggtt gctgttgcaa ataaagatag cgtagcacaa gttaagatta agtcagcaga
    23221 agaagtgttt acagaagtat taaatgagat agaaaagaaa cttataacta tagatatgat
    23281 aaatttcttt atatcagaaa aagcatttgt tgtagaagat gctgatataa aatcattaaa
    23341 ggaaatacta gataaatttg aattagatta tgaagtaaat agagactgtg ctaaggtaac
    23401 attgatatgt tcaagaattg ctgatgaaat gtctggtatc atgtcaaagg tagttagagg
    23461 attatcaaaa gcaggggtat cccttttaca aacatcagac tcaaatatga ctatatcttg
    23521 tttagttagt gaagaagata tgcatacagc agtacatgct atacatcaac aattttattt
    23581 aaaataaact gtataataca attatttttt aatcaaattt aagaatttca tatatatctt
    23641 aatatatttg atttatttgg taaaactaaa ttaaatataa ttttgatagg agttttttta
    23701 tgaaagatta tagagaagat aatgaaagat ttgataaaga taattgtagt gaagaatcta
    23761 gttgcgaaga gagagaatgt ataaagcaat ttggtaccaa tgaaatgcca cctcaacctc
    23821 ctaaagatat tcaatgtata actataattg gagaaattga gggacatttt ataggaaatc
    23881 ctcaaaaaaa agctacaaag tacgagcata taattccaat gctatactca attgaggaaa
    23941 gtaatgatgt taaaggagtg ttagttgttt taaacactgt tggaggagat attgaagcag
    24001 gtttagctat tgcagaactt ttaaacagta catctaaaaa agtagttact cttgttctag
    24061 gaggaagtca tagtataggt gttcctcttg ctacagcagg agattactca tttatagcac
    24121 caacagcaac catgattata catccagtgc gaactactgg tcttgtgatt gggataaatg
    24181 agacttttga gtattttaaa aagatgcaag atagaataat tcaatttata ataagaacat
    24241 caaatatcaa aaaagatgta cttgaaaaac ttatgcatga aaaagatgaa cttgtaagtg
    24301 atgttggtag tgtattaata ggaaaagaag ctgtggatta tggtcttata gatgaagttg
    24361 gaggactaaa agaagcactt aaaaaattaa gagagcttat aaaagaatct gaggaagaaa
    24421 aaaataatga ttaaaatttg tttaaataat aaaatttatg atataatgat gtaataaagt
    24481 aggagtgtta gtgttaaggc attagcactt tttgctgtga aggggtaaaa cttgctaaaa
    24541 ataaggagtg atattaatgg ccacgaaaaa gaagaaaaaa aagaaggtaa ataccttaag
    24601 ttttaatgca gaatatcata atttaataac aatatttata ggtgtatttt tattatatag
    24661 tttaaattct aattctatgg gatggatacc tgtgttaatg caaaatcttt ttaagggact
    24721 atttggaggg ctttcaatag caataccatt tatagtaatt ataacaggac ttttaggatt
    24781 ttttgatgga aatgaatata tatataggct aagaaagact aaactatact acataattat
    24841 tttatttata tttgtatttt atggattatt aaatgcagga actcttcctg tagatagccc
    24901 actaaaaggt aacatgtttg atgatgttat gaaattgggt gtaagtggac aaggtagtgg
    24961 tttgatagca acaactattg cgtattatat gagtaaaata tttggtatag caggtggatg
    25021 gttaataagt atcttcgcac ttatactttc tgtaatgttt atatttaata tttctataaa
    25081 agatttggtg tcaaatgcaa aatcaaaatc taaagcatct aaagacagca atttaacgtt
    25141 taaagataaa attgcaaata tgaaaaaatc tgcaatagat atgatgactg atgaagtaga
    25201 tgatactact ataaataaaa aaccaggttt ctttaaaggg ttaatgtcaa aaggtcttaa
    25261 taatgatgat gaagaagatg aatattttga agaagaaaat agtgatggtg tagatgataa
    25321 aactataaaa atagttggat ttaataaggc agaagatgag tatttagaaa tattagaagg
    25381 aactcaaagt atgccagagt tagatgtttt aaaagaatta caaaaagcta ccaatgaaaa
    25441 tccagttgta gatactaaac cagaaaaaaa agttgatata gctaaaccta atttaaatat
    25501 agaaaaaact caacctatga gtattgtagc agagccagtt aatgaggatt attcaaacta
    25561 taaaaagcct agcattgagc ttttaaataa agtaaataag aagtctgatg aaaatggtaa
    25621 aaagaaagtg ttgaaaaatg cttctctttt agaaaagaca ctatcagatt ttggagtgga
    25681 agcaaaaatt aatcaagtta cagttggacc tactataaca agatatgaaa tacaaccaag
    25741 tccaggagtc aaggtaagta aaattgtaaa tttaactgat gatatagctt taagtttagc
    25801 agctaaaagt ataagaattg aagccccaat tcctggtaaa agtgcaattg gaatagaagt
    25861 tccaaatgaa gaagctcaaa tggttggtgt gagagaagtt ctagaaagtg aagaatttaa
    25921 taattttaac tctcctcttg caatgggact tggaaaagac gtagcaggaa aaattataat
    25981 tggagatata ggtaagatgc cacacttact aatagctggt tctacaggtt caggaaagag
    26041 tgtgtgtgta aatacattaa taagtagtat tttatataag gcaaatcctg atgaagtaaa
    26101 attactatta atagacccaa aagtagttga acttgcaaat tataatggaa taccacatct
    26161 attgatacca gtagttactg accctaaaaa agctgcaaat gctttaaatt gggcagttac
    26221 agagatgaat agaaggtaca aattatttgc agatgctcaa gtaaaagatg taacaagtta
    26281 taatgagaaa gcggaagaaa aacttcctaa aatagtaata ataatagatg agttagcaga
    26341 tttaatgatg gcaagtgcaa atgatgtaga agattatatt tgtagattag ctcaaatggc
    26401 aagagctgct ggaatgcact tgattgtagc aactcaaaga ccatctgttg acgttataac
    26461 aggggtaata aaggctaata taccatctag aatagcattt gcagtatcat ctcaaactga
    26521 ttcaagaaca atccttgata tgggtggtgc agagaagctt ctaggtaaag gtgatatgtt
    26581 attctatcca cttggtgcag caaaaccagt tagacttcaa ggggcattca tatcagagag
    26641 tgagtcggaa aaagtaatcg attttgtaaa aagtcaagtt aaagatggaa taaaatatga
    26701 agaagatata attgaaacta tatctaaggt taatacttca aagggtagtg atgaggatga
    26761 gtttttgagt gaagcgatag aatttgtcgt ggaaagtggt caagcatctg cctctatgct
    26821 acagagaaga tttaaaatag gattcaatag agcagctaga cttatagatt ctatggaaga
    26881 aagaggaata attggagcaa gtgagggaag taaaccgaga aaggtattaa taagcaaaca
    26941 ggatttgcag aatttagaag gtgaatagaa ttgtcagtta taaaaataga aaaagcactt
    27001 aagctagata aagtaatatt tgtagatgtg agaactgaag gagaatatga agaagaccat
    27061 atattgaatg caatcaatat gcctcttttt aaaaataatg aacataatga cgttggaaca
    27121 atttataaga tgcagggaaa acatgaagca atacaaaaag ggtttgacta tgtatcgtat
    27181 aaattaaagg atatatattt acaagcagca gaacttgcat taaattatga taatattgtc
    27241 atatattgtg caagaggcgg aatgagaagt ggcagtatag taaatttatt atcatcatta
    27301 ggtgtaaatg tatatcaact tgaaggtgga tataaggctt atagaaattt tgtattagag
    27361 tatctaaggc atgtaatgga tacaaaaaac tttgtatctg ttcatggatt gactggtaca
    27421 ggaaagactg atttgttaca tttattagat gaaaaaggaa tagatgtact ggatttagaa
    27481 ggcatagcta aaaacagtgg ttctaccttt ggttttataa cttttgataa aaagccacct
    27541 tctcaaaaga actttgaaac aaaactattt gaaagtatat ttttttcgaa agagaattat
    27601 atatttgtcg agagtgaaag caaacgagtt ggaagtgtta cagttccaaa tgaaattttc
    27661 gaggcaatga cacgagaagg acatcatata ttattagaat gtgatattga aaatagagtt
    27721 gatagacttt gtagagatta tatttatgat aaagatgaag gtaatatatt gattttaaaa
    27781 gaatgtataa acaagtttag aaaaagattg ggtaataaag tagtagatga ttatattgat
    27841 ttattagaat caggtgaata taaagaactt gtaaaaagat atcttttaga atactacgac
    27901 ccactttata tgcattcagt agaaaaatat aagtatagta agattataaa ttttaatgat
    27961 acacaaaaag ctttagaaga tgttatagac ttatatgaga gtatattgga aggagaaagt
    28021 gaatgttaaa aatagcatta gaatcattag gatgctcaaa aaatttagta gatgcagaaa
    28081 taatgatggg aattttaaat aacaaaggct ataaattaat tggagatttt gaagaagctg
    28141 atgtaattat agtaaataca tgtggattta tagagtcagc taaacaagaa tctatagata
    28201 cgataataaa ctttgcagag ttaaaaaaaa ctggcaattt aaagttgttg atagtaacag
    28261 ggtgtctagc ccaaagatat tctgaagaat taaaaactga aattcctgaa atagatgcta
    28321 tagtaggaac aggaagctat caaaatatag ataaaatact taaagaatta agtgaaatgc
    28381 ataaaattgt aagccttaat gatatagagt ttgtatttaa tgaagattta ccaagatata
    28441 tatctacacc tagctatatg gcctatttaa aaattggaga aggatgttcc aataattgta
    28501 cttattgtat aataccaaaa ctaagaggta aatatagaag tagaaaattt gaagatataa
    28561 ttaaagaagc taagaaatta gctgaatctg gagtaaaaga acttgtggtt atagcacagg
    28621 atacgacaaa gtacggtttt gatttatatg gaaaagaaag attatcagaa cttttagaag
    28681 aacttgcaaa aatagatgga tttaagtgga taagggttat gtattcatat ccagaatcta
    28741 taacagaaga attgatacaa gttataaaga aatatgataa tatatgtagt tactttgata
    28801 tgccaataca gcatgcaagt aacaatattt taaaattaat gaatagaaaa actactaaag
    28861 aagatatatt aaataaaatc aatctgataa gaagtaatat accagatgct atacttagaa
    28921 ctacaataat agtagggttt cctggtgaaa cagaagatga cttcaaacaa cttgtggact
    28981 ttgttgaaga ggttaaattt gatagacttg gtgcatttgc atattcaaga gaagaagata
    29041 caccagcaga tagattgcca aaccatatag atgaagaagt aaaaatacaa agaagagata
    29101 ctttaatgat gattcaacaa aaaatatctg aagaacttaa tgacaaaaag ataggaaaaa
    29161 cttatgaggt gttaatagaa gaacagatag aagataatgt atatacagga agaacacaag
    29221 gtgatgcaga ggaaattgac agtatagtgt atgtaaaaag tgtagacaat cttgaagtag
    29281 gagaatttgt aagtgttcaa ataaatgatg caatggaata cgatttaatg ggagatgttt
    29341 tatatgaact tgcctaacaa gttaactttg tttagaatat ttttaatacc agtatttgtt
    29401 ttaattatgt tgctcaatat accaaataaa tttttaatag cttgtattat ttttataata
    29461 gcttcaatta ctgatgcttt agatggaaaa atagctagaa aatacaatct agtcactgac
    29521 tttggaaaat ttatggaccc attagcagat aagttattgg ttatttcagc acttacatgt
    29581 atgatagaag accatcttgt aagtagttgg atggtaatta taatagtggc tagagagctt
    29641 acagttagca tattaagagc tatagctgct gctgatggaa aagtaattgc tgctggaaac
    29701 agtggaaaac ttaaaacgat aactcaaatg gtatctatag tgtttttatt gttaggtgct
    29761 cagtttgaaa atgtattgat attaaatatt ggtgaaattc tcattcttat agctacacta
    29821 cttacattat attctggatg ggaatatcta tataaaaata aagagctttt tatgtcttct
    29881 aagtaaaaaa ttatgcttta ataaataaaa actaatgatt aataaataaa aaataacaac
    29941 ttaaaatttt aaaaatgtaa aaaagggtat actattatag tatacctctt ttcattttaa
    30001 gaatattttc ttttgtgtat ataattatta ataatggttt ataaataaaa tgtaatgaat
    30061 aaaaatataa tcattagtag cgtttttaaa ttgactaggt ttgatttatg ttatataatt
    30121 aatatataac aagagaacaa atgtttgtag ataaatgaag ggatggtata gagatgagtg
    30181 tagatcaaga aaaattaaaa gcgttgaatg aagctttagg taaaattgaa aaagattttg
    30241 gtaaaggttc agtaatgaaa ttgggagaag caacatctat gtctatagat gttatatcaa
    30301 caggagcgat tggtttagac atagctattg gtataggagg tctacctaga gggagaatag
    30361 ttgaagtata tggtccagaa tcttctggta agactactgt tgcgcttagt tgtgtagcat
    30421 cagctcaaaa agatggagga atagctgcat ttatagatgc agaacatgca cttgacccag
    30481 tatatgcaaa agctttgggt gtggatgttg ataacctaat aatatctcaa ccagatacag
    30541 gtgaacaggc tttagagata gcagaggcat tgataagaag tggagcgata gatataatag
    30601 taatagactc agtagcagca ttagttccaa aggctgaaat agatggagat atgggtgatt
    30661 ctcatgtagg tttacaagct agacttatgt cacaagcact tagaaagtta actggttcaa
    30721 ttaaaaaatc aaattgtgtt gctatattta taaaccagtt aagagagaaa gtaggaataa
    30781 tgtttggaaa cccagaaact actactggag gacgtgcact aaaattctat tcatcagtta
    30841 gattggatgt tagaaaaata gatacaataa aacaaggtga taaagttata ggtagtagaa
    30901 ctagagttaa agttgttaaa aacaaagtag caccaccatt taagcaagct gaatttgata
    30961 taatgtatgg agaagggatt tcaaaaattg gagacctttt agatatagct gctgatgtag
    31021 atatagttaa aaaatcaggt tcatggtata gttacaatga tactaaactt ggacaaggaa
    31081 gagaaaatgt taaaaaattc ttggaagata atttagattt aactactgaa atagatgaga
    31141 aagttagagc attttacaat ttaaatgaag aacatgaaga atcaggtact tcagtatcaa
    31201 aagaaattgt agaagaataa gtaaaaatac aaaaaaataa ctagtaatta aagacatgcc
    31261 acgctcccta ttagcttgac acaatgttga aaaaattata aaattaagtg aggataaaat
    31321 atctaaattt aattttaatt attaggaata ttttttaatc atagtgatga cattttggga
    31381 ttattaacga aggaggtgga tgtcatagat agtgtagtaa tgattgtaat aggtgcagct
    31441 gttggtatta tagctggata ttttgttaga aaaaatatat ctgaagcaaa aattggacaa
    31501 gctgaaaact tggccaaaga aataatagat aaagcacacc acgactcaga aacagtacaa
    31561 aaagaaaaat tactagaagc taaagaagag atccataagt ggagaacaga agctgaaaga
    31621 gaaaacagag aaagaagaac agaagtacaa aagtatgaaa aaagagtagt acaaaaagaa
    31681 gaagtcttag atagaaaatt acaaaattta gaatctaaag aagtaaacct aagtgaaaaa
    31741 ttaaaggtag tggaaaagaa agaagaagaa gttgaagtaa taaaaactca acagttagaa
    31801 aaattagaaa gtatatcagg aattacttct gataaggcca aagaaatcat cttaaccaat
    31861 gcggagagag atgtaagacg tgaaatgtct ataatgataa aagagataga aagtcaagct
    31921 aaagaagaag ctgataagaa atcaagagaa attataggat atgcaattca aaaatgtgca
    31981 gcagatcacg tagccgaaac tactgtaaca gtagtaagct tgcctaatga tgaaatgaaa
    32041 ggtagaataa taggtaggga aggtagaaat ataaggactt tagaaacctt aacaggtata
    32101 gaccttataa tagatgacac tccagaagcc gtaattttat caggatttga tccaataaga
    32161 agagaaattg caagaattgc tctagaaaaa ttaatagcag atggaagaat acatccagct
    32221 agaattgaag aaatggttga aaaggcgaga aaagaagttg acaatattat aaaagaatat
    32281 ggtgaacaag ctgcatttga aactagtgta catgggattc atccggaatt aataagatta
    32341 ttaggtagac ttaactatag aactagttat ggacaaaatg tacttaaaca ttctatagaa
    32401 gttgctcata tagctggtat aatggctgct gaaataggag cagatattag attggctaag
    32461 agggcaggtt tattacatga tataggtaag gctgtagacc atgaaatgga aggtacacat
    32521 gttgaaatag gtatggattt acttagacgt tataaagagt ctaaagaagt tatacatgcc
    32581 atgagtactc atcatggaga ttacgagcct caaacagtag aggctgtatt agttacagca
    32641 gcagatgcaa tatcagctgc tagacctggt gcgagaagag aaactttaga agcatatata
    32701 aagagacttg aaaaattaga agaaatagct aattcatatg aaggtgtaga aaaatcattt
    32761 gctatacaag ctggtagaga aataagaata atggtaaaac cagaagctat aaatgatgaa
    32821 gaaatacatc tattggctag agatatgact aagaaaatag aggatgaact tgaatatcct
    32881 ggacaaataa aagtaagcat aataagagaa acaagagcta tagagtatgc gaagtaaaag
    32941 aaataatatc taaataataa gaaaatattt ttagaaattt aaatttgcca caagatatga
    33001 taatatatct tgtggctttt aaattgttta agtttaattt tatataatgc aaaaataatc
    33061 taagtgctaa aagtgctact tacaaaagat taataaaata aagctttata tatgtattat
    33121 ttatataatt atccaagttt gtttgatata atgaagttgt aaaataaata tattactact
    33181 tggaatgctt gtacttaaat aagatgaaaa tacaatgacg ctaaagtgag gattagagag
    33241 agggtacact atgaaaaaaa atacactttt acatacaaat attatggttt gcattattat
    33301 tactcttggt tttgtggcta cctctataat tggatatcaa tctaatacca atatttttaa
    33361 aaatgatatt gaacatgtat ctacattggc tgctgaaggt atttattatc agatagataa
    33421 actactttca gaacctataa atgtatctct tacaatggca aatgacagtc tattaaaaaa
    33481 ttttttagat ggtgagaaag aacatttaaa tgatgaagaa tttatttata aattacaaga
    33541 ttatctgaat gtgtatagaa ataaatattc atatgattct gtttttttag tatcaactaa
    33601 aaccaataat tattatcatt ttaatggact tgatagaaat cttagcgcta ataactcaga
    33661 gaatcaatgg tattatactt tcttaaagaa tgatgatgaa tactcattaa atgttgataa
    33721 tgatgaagca agtaacaata gtattacagt ctttgtaaat tgtaagataa aagatgataa
    33781 tggagcaact atgggaatta taggtgttgg gttaaaggtt aattcattac aaatgttgct
    33841 taaaggatat aatgacaaat ttgatgttgt tgcacgctta attgatgata aagggtttgt
    33901 acaattagca gtagataaaa ccggtcatga gaatataaat ttttttgaaa attctagtag
    33961 cgacttgtct aattcgaaac aattaatatt aaacaacaaa aaggaacaaa agagtttttg
    34021 gtattcatct gaaaaatcaa aaagttatat tgtatgtcag tacataccta gtttaaaatg
    34081 gcatcttgtt ctagaaaatg attttacttt aatgattaaa cagcttcatc tgcaattttt
    34141 taaaggcatt gcagttatag ttttaattat aatatttgta ctttttacta ttacacatgt
    34201 gcttaaaaaa tacaaaaacc aaataataaa gcttagtgtt tcacagaaat tggaatatca
    34261 aaaattgttg agcaaatcta ctgaaggtct ttatgaaaat atttttgaaa ttgatattac
    34321 taacaataaa gctggttgtg aagatgagaa taggtatttt catgaatttg gattagggct
    34381 taatacccca tatgatgaag ccttacatac aatagctaaa aaccaaatca aagaagaata
    34441 tgcacaaggt tatttagata catttttacc tgaaaatgta attaaacgtt ataatagcgg
    34501 aattaataat atcagctatg attttttaat taaaaaagat gaagaaagtt atcattggat
    34561 tagaattaat gcacgaattt tcttttggac atcagatgaa tctgttcgta tgatatctta
    34621 tcgtaaaaat atagatgaag aaaaaaatag agaattacta cttctcgaac gttcacaacg
    34681 tgattctctt acagggcttt acaataaagc tacaacagag gaatttattc gttcattttt
    34741 aaaaaatgaa agtaatcaag atttagaaca cgtttttcta attttcgata ttgataattt
    34801 taaaaatgta aatgataaat atggtcatgt atttggagac tttgttataa gtgaatttgc
    34861 cttagaatta aaatctcaat ttcgtgagga cgatattgtg ggacgtattg gtggagatga
    34921 gtttgtagta ttaataaaag actttaataa taataactat gtgcttatag acaaattaga
    34981 gcgtttttgt tctaagatta atcagaaaaa atttgttgag caagcagaat tttctattgc
    35041 atgtagtatt ggtgtagcaa tgtatcctga acatggatat gtatactctg aactatatga
    35101 gaaagcagac caagcacttt actatgcaaa aagccatggg aaaaattctt atcatatatt
    35161 ttcagaggat tctattgata gtatttcatt tcatatagac tctagagata taaaagtatt
    35221 attagaaatg gctactgatg gtgttgtcaa atttgcttgt atggaaaact taaaattact
    35281 ttatttcaac caaaaaatgc ttgaatttac tggatattca gctcaaactg ttacttctat
    35341 aggttttgat attttttcgc ctgtattaca agacgactta caaatggtac tagatacatt
    35401 aagagaagca atttctaaaa gagagacttt tgaaatagtt tttcgttttt gtcatacaga
    35461 tggtcatatt tttcctgtaa ggatgcaagg taagtttgta agtgaattat atcaaaatcg
    35521 ttatcctgtt ttttatgcga tttatacaga catatcaagt atcaatactt tactagaaaa
    35581 taaatagtaa atactacagt aaaatctaaa aataggatat aatagtatta atataagaag
    35641 attataaata aaacctgcta atgaaaatag attattttta gcaggttttt aaatttttta
    35701 taataaatct tatttttggc taacaagatt tatttttttt aagactttac ttattatttt
    35761 aagcattaat tatgaaattt atcattttat tatttacaga aagtatttta taacaataaa
    35821 tatctatact tattgcaaaa tttttttagc agttttgtat ttatttgtat ttattttgaa
    35881 tttatttact aaatttgcct ataaaaagta tatataaaaa tatagttcaa aatggtataa
    35941 ttaagtataa cagaaggagg taatgtaagt gagccaatac ataaatataa attctcagtt
    36001 agacgcccta aaattgaagg agttcgagaa aatattttac gaaaatatca atgaggacgt
    36061 taaagtagtg ccaaaagtta ctccttttaa aggaataaat accgatttgt tgtatgttaa
    36121 agatggaaga attttattta taaaatttat ggatacaaca gaagatatat tttttatttt
    36181 agaagaagag ttacttgaag ttatgaatga ggaatatgaa cttttgaaac taaaaatggg
    36241 acaaaagaat agaaatataa gttataacta cgtttacatt atgccatacg ttgaagttga
    36301 ggaaacatat gaatttgaag aatttgttaa taataatata atagataaaa ataagcttca
    36361 agatataatg aataagggtt cgctggagga atatcttaat gatgagaatg atgaaataaa
    36421 cttaaattta tttttactag acatatgttc agaatactat ataataaatg ataaacttca
    36481 tctaaatgaa aaatttaaaa aaatttcatt ctacaatgat gactataagt atacagcaac
    36541 tatgatggaa gacgttcaaa ttaaagatgt tgtttctatt aaatatggga atacacttat
    36601 tgaaggtggt tctggtatag gcaaaacagc aattatgctt tctagggcta taaaattagc
    36661 aaaagtatat ccacatcata aattagtcat atttacacat acaaaacaac tgtgcaatga
    36721 gcttcgagag aggatagagt tattatacaa agataataac aatttagaag ttcacacatt
    36781 tagttctttt atatttaaac tagctaaaaa attcaattta attgtagatt ataacatgtt
    36841 aaaaaatgat tacgaaaaga cctttaataa tcttgtaaaa caagcacaga acattataaa
    36901 aaataaaaat atgttcaagg ctatttttat tgatgaagca gaaagctttt tagagtatga
    36961 aatagatttt ataagggagt ttttatataa aacaaaattc atatttaatg tgtgttcttg
    37021 taactccttg aatatctcta atagattgaa tatatttaaa aaattatata atggtataga
    37081 atttgatgat aagattatct taagtaaaaa ttatagacaa gctaaagaga ttgtagattt
    37141 tacaaataaa ttttcaaata attctaactc atatatcaac gaattaagac caaatacaga
    37201 atttagtact ttcttttata ctaaagcact tagaggtgga aataagtctg ttgatataat
    37261 aaaagtaagt gaccttgatg accaaataag ttctgtaatc tgggaaattg agtatttaat
    37321 aagtaaaaaa ggtcttgatt attctgaagt tgctatcgtt tatccttaca ataagaagaa
    37381 attaaaaagt ggaaaaacca tttactttca atatatgtta aaaaaagcat tggaagaggc
    37441 aaaaattcct tatatatgtg cagaagataa tttgacaaat ataagtaaga aggtaggaac
    37501 tacaatagca aatatatatg caataaagaa tttagagtat aaagcggtaa tagtatgtga
    37561 attagagatg ttatacaatc aaactataaa tgatattgaa caagattatc aagtaaatga
    37621 ctttgtagga gatttaaata aggtttattt agctatgagt agagcaacag actacctaag
    37681 tatagtaaca acttttaatg aagaagcaag tgatataata agacttatta cagaatcaaa
    37741 agatatttag taaaaaaata tttgtaattt aagaaaataa aaagagactg agatttgtaa
    37801 aataaacttt accaattcag tctcttattt tttagtctac tttcccaata ttatcaagag
    37861 gtaatagtct caccataacc ttaccaagaa cttcatcttc atcaaccata cctacatcag
    37921 ggaatctact atcattacta ttttctctat tatctcccat tgcaaatagt ttaccttttg
    37981 gaacaacagt atctatatct ccagaagtat agttattgtg tatatatggt tcatttaata
    38041 attttccatt tacatacact ttagaatttg atatttttat tctgtcacct tcagtagcta
    38101 taactctttt tattaagtct tttttctttc ctgtttctcc atcaactaaa ttggttttaa
    38161 aaactacaat atcgcctttt tcaggtttac caaccttgta tgatatccta tttaaaatca
    38221 gataatcttt atcatctaaa gtaggataca ttgattctcc acttacaata gaaggtctta
    38281 taaattgtag aattacaaat gcaagtacaa tagccatagc tattgactta atccaatcaa
    38341 atatttcttt tttaacactc ataacttttc tccttatcat ttgttattaa attagaatac
    38401 attataatag aaagaaataa gcaagaacca ctatacccaa gactattcta taataaccaa
    38461 atactttgaa gtcatgtttt ttgatatagt ccataaaaaa tttaataact acaattgaaa
    38521 caacaaatgc tactacagaa ccaacaccta aaataagcca ttcaaatcca gtaaatccaa
    38581 atccagcttt tagaagtttt aatgcactag ctccaagcat tgtagggatg gctaagaaga
    38641 atgaaaactc tgctgctaca tatctagaag taccaagtaa tactgcacct attatagtag
    38701 aaccagacct agatgtacct ggtataagag ctagacactg gaataatcct atacaaagag
    38761 ctaatttata tgtaacttga gaaaaatctt tatacttagg ttttttattt ctcttttcaa
    38821 gccctatcat tattatacca tatacaataa gagcaatagc aactactgtt gaattaaaga
    38881 ataatctatc tatatcatct tcaaacagta ttccaataac tccagatggt ataactgcaa
    38941 ttataacctt aagccataaa cggactgttt cttttttctg cttaatattc tttgcgctat
    39001 caaaaggatt taattttcta aaaaatataa caagtacagc taatattgaa ccaaattgaa
    39061 ttacaactaa aaatgtactt ataaatgtat ttgaaaaatt caattttata aactcatcaa
    39121 ctaatatcat atgccctgta ctacttactg gtagccattc tgttattccc tgaactatgc
    39181 ccaaaatgac tgccttaaaa atttcaagca aactaaatgt ttccaaatca aaatacctcc
    39241 aatacaaatt tatagtagca tattttattc atagattaat aaaaaaatgc caccttttac
    39301 taacttataa taccatagta ctgtgtagtg cacaatggaa gattgatatt ttcataaaac
    39361 cttcataata taacaaaata aacttaaaag ttatctattg aataaggggt atatttgagg
    39421 gggaaatata tggtatagta aaagtatagc tattttattt tatgaaaagg aaaggagtta
    39481 ttatgtatag tacaaaaata ctagatgatt atgagtatga aacttttaat gttttttcta
    39541 caaaattaaa tccaaacacg aaacacgatt atttatcaaa ggttatactg tttaaagaat
    39601 ttttaaaagg gaaagaattg atttatgcta cgaaagaaga ttgtaaaaat tttgtagact
    39661 atatacagac taaatatgcg aaatcaactt gtgaaaagat atatagttat ttacatagtt
    39721 tttataactt cttaaaaaaa gaaggataca tagatataaa tccatttaga tatgtagaaa
    39781 aacccacagt aacaagaata aaaacaaaag atgatgttct aagtatacaa gaaataaata
    39841 aacttatagg tatcctacca aaattaaata taagagatag agtaataata gtctgtctag
    39901 ttactactgg gtgtttatta aatgaattag ttagcttaaa gtggaaagac cttatggtag
    39961 atgaaaacga taactcatat gtaaggttag gtaagggaag aaaagaaaga gtagttaaat
    40021 tacatccata cttttttaaa cttttagaag attacaggaa ttattctggg cttccagagg
    40081 taataatacc tagtgatgat tttattttta caactcaaaa gagtaattct ataacagata
    40141 gaaatgtcag acttatagtc aaaaaagcat tagatttagc tggactttct caatattcag
    40201 caagagattt tagacattca tttgcagcaa taagtttaag gcttggggca gatgaaagtg
    40261 atgtaaaaaa tcagcttgga tggagtgata aatactatgc aattagatat aagtatgtat
    40321 taaattttgt tgatagtgaa atagtggatt acttaattga aaaagataat ttggatataa
    40381 ataaaaaata gtaataaata aaaaatacta tatataaatg tgaacaaaaa ataaaaaagt
    40441 cttgttatag ttcgtaaaat tatatataat tatttatgta ctaatgtgta cacagtaatc
    40501 acacgacaaa agatttaagg aaggtgctaa tataatgata gataataaat atactactta
    40561 ctcaaaccca cttacagaca gatattgtag caaggacatg agttatatat tttctccaca
    40621 atttaaattc tctacatgga gaaggttatg ggttgcactt gcagaaggtg aacatgagtt
    40681 aggaatcaat ataactgaag aacaattaga tgagctaaga gctaatatag ataatataaa
    40741 ttttgatgaa gctagaaaaa tagaaaaaga agtaagacat gatgtaatgt ctcacgtaaa
    40801 agcttatggt ttacaatgtg aaaaagccaa aggaataata catttaggag caacatctgc
    40861 atatgttgga gataatacag atgttataca aatgaatgaa gctttaaaac ttctaagagt
    40921 aaagcttgta aatttaataa acaatttaaa agaatttgca cttaagtaca aagatatgcc
    40981 agctcttgga tttacacact tccaagctgc tcaacttaca acagtaggaa aaagagcttg
    41041 tttatgggct caagatttaa ttcttgattt ggaagattta aactatagaa tagacaacat
    41101 gaagttaaga ggagttaaag gtactactgg tactcaagca agttttttaa gcttatttga
    41161 aggagaccac gaaaaagtaa aagagttaga taagaaaatt tgtaaaaaga tgggatacca
    41221 ttcttcctat gcagtaagtg gacagacata tactagaaag ttagattatc aagtaataag
    41281 catactatca gggatagctc aaagtatgca taagatgact aatgatataa gacttttaca
    41341 aagtttaaaa gaattagaag agccatttga aaaaaatcaa ataggttcat cagcaatggc
    41401 ttataagagg aatccaatga gaagtgagag aatagcatct ttatctaagt atataatctc
    41461 tgaatctata agccctgcaa tggtacaagc cactcaatgg ttagagagaa cattagatga
    41521 ctcagccaat aagagattgg caatacctca agcatttatg gcagcagatg ctatacttga
    41581 aatagggata aatgtaactg atggtttagt agtatatgaa aatatgataa atagacgtgt
    41641 aaatgaagaa cttcctttta tggctacaga aacaatactt atggaagctg taaaaagagg
    41701 tggagataga caggaattgc atgaaataat aagagaatat tctatgaaag ctgcctatag
    41761 agtaaagcat gaaggcaagg ataataactt aattgaactt ataatgaatg atgattcatt
    41821 taaaatgagc aaggaagaaa tactatctat aatggaccca aaaaacttta taggtagagc
    41881 tccagaacaa gttgtagaat ttgtaaatga agttgtagaa cctgctataa aagattataa
    41941 ggaagactta ggaaaagtag atgtagacct aagagtatag aattgtacag ataaaatata
    42001 attaaaaacg gctatcttat ttataaataa gatagccatt ttaatgggct agcgtcttct
    42061 ctccaccgct gtgaagagtt cttctttact tactatacca tgaatttaat aaaatagcta
    42121 tataaataat atttaatagg aataatataa ataaaaataa aaaaatgata agattactaa
    42181 ttaaatttaa acattcagca aaaactattg tacttgcaat atttttaatg taaactgaaa
    42241 taaagttatt tagataattt aataaatttt aaaacaaccg aggtgattat atgaactata
    42301 tagtaaaaga taccataagt tttttagtaa aaacatttcc taagatttat tctagcctgt
    42361 acttagaaga cctaaaaaaa tttgcaccag attataatgt aaataaaaca caattaagag
    42421 cattagtttt cataaaaaat tatggagtaa taagtatgac tgatttatgc tctaagttaa
    42481 atatagaaaa aggcagttta actagtatgg ttgatgattt aactgataag aagtatgtaa
    42541 caaggaaaag agatttagtt gatagaagaa aatatctaat tgacattact gaagagggag
    42601 ataagatagc aactgatttt atggataaat taagtgatgg tttaggagaa aaactttcta
    42661 aattaactga agaagacaga aaaaaatatt tagaagcaat aaatactctt caatatatat
    42721 taaacaaaga agaatttaga tagaaaaaat ataaaaattt ttgtatttaa tggtactaag
    42781 attaattctt tgttattcat ttaactatat aattataagt ctaaatacaa tctaaaaagt
    42841 acatcttaaa ttttcttgat gtactttttt ataatttctc taactaaggc aagacaagtt
    42901 ttagatgctt taattaaagt gaataaagat tactggccaa cactatataa agaaaatgaa
    42961 aaatagtcag gtctaagtct taattaatat attaattaag aaatgtcccc ttttcaaata
    43021 atatctgaaa aggggacatt ttatatcatt aaatttatat aaattaagtt taaaaaatta
    43081 tttttgtaaa gctacatatt gctctaaacc ttcttttaat aatgtagcag ctctatgaag
    43141 gtctttttca tttaagatgt aagctaatct tatttcatca cgtcctaaac ctggagtagc
    43201 atagaatcct tcagcagggc aaaccataac tgtttcatta tctttattaa attcttttaa
    43261 catccaaatt gtgaaatctt ctgcattttc aactggtaat ttagcaacta tatagaaagc
    43321 accagttggt tttttacata taacaccttc aaccttcatt aattcattgt ataatacatc
    43381 tcttcttttt ttgtactctt cattaacttc tttgaagtaa gaaacaggag ttttgtataa
    43441 ctcaacagct cctatttgct ctaatgtagg aacacaaagt cttccttgac ataatttaag
    43501 tatttgagcg ataagacctt tatttttgca agcgatagaa ccaattctag caccacaagc
    43561 actgtatctc ttagaaacac tgtctattat tataactcta tcctgtacac cctctaagtt
    43621 accacagctt gtatactcaa gcccatcata aacaaattct ctataaactt cgtctgctat
    43681 tatccataaa tctttttctt tagctatttc agctaggata ttaagttctt cttttgtata
    43741 aattgcacca gttggatttc ctgggttaga aagaattata gccttagttt tatcatctac
    43801 tttagacaat atttcttctt tagatggaag gtggaaaccg ttttctgcct ttgttgtaac
    43861 agcattaact tctacgttta cagattgacc aaatccattg tagtttgtat agaatggttc
    43921 tggaactaat agattatctc ctgggtcaca aactgccatc atagtgaaaa gtaaagcttc
    43981 actaccaccg ttagttacta ataattcatc tttttcaaaa ttcatatcgt aagttttata
    44041 gtaattttga agtgcttcta ataattctgg aataccttct gatgtagcat attctaagac
    44101 ttcgctatca aagtttttaa cagcatcaaa aaatccttta ggagttttta tgtctggttg
    44161 tcctatgttt aagtgatata ctttgattcc tttatctttt gctgcttgtg caaatggaac
    44221 aagttttctg attggagagg cttgcattgc actaactctg tttgaataat tcattttaat
    44281 accccctatt taatttaaat acgtatattg tattatgtaa attgtatgtt tgaatacaag
    44341 acatctttac aagataataa taacatactt atgggtaaaa atcatcaaaa aaaattaata
    44401 tagctttaaa ataaactttt aacaaattat agtaaaatac ccatgtttat gccactaaat
    44461 tatgtattca taaattaatt tatagtaaga taatattctg atgtactgat agtgattttt
    44521 tagttataat ctttcagcta tagacaaaat atataataat aaatatgcaa aattataaaa
    44581 aatattataa atttatttaa cagatacgat aaatatcata taattatatt aaaggctatt
    44641 aactaagagg tgttaattat gaaagaatta agaatagatc caattaccaa tgatgttgtt
    44701 atatttgcta cagataggtt aaaaagacct ttagatacag cagatatccc aaatgaagat
    44761 gaagaaacta atgaatatga tgaagaatgc ccattttgca aaggaaatga agctcatgca
    44821 actgatgcat tatttgaaat agatagtgaa gaaggatggc ttgtaaaatc tgtctacaat
    44881 aaatttccta taattgatga tatggcaaga gatgtatatg gtgttcatga agtaatgata
    44941 gagagcgata aacataatcg tagtttttat aacatgagtc aaaaagaatt tgaagatgtt
    45001 ttttttatgt atagaaatag atttagagat ttgtcgaaag atgataaagt agagtatgtt
    45061 agtatcttca aaaacttctt gagaaaggca ggagcatctt tgatgcatcc acatgctcaa
    45121 atattgtcta tgtcttttat tccacctgaa attacgaatg aactgttggt ttcaaaggag
    45181 tattatgaca gcaataaaag ctctttgtat gatgatttaa tagaaaatga aataagttta
    45241 gatagaagag ttgtttataa tggagaagct tttttggtac ttataccata tgcaaccaag
    45301 tatagtggag aagtaagaat aatatttaaa gataaaatag agtttggtga gttaaatgat
    45361 aataatatac aggagctttc tacaatattt gaaaagttgt ttaagaagtt atacaatatc
    45421 aatggatata tgccatttaa tttatgtatt cacactcatc caacaaagat tgaaacaaaa
    45481 tcgtatttta atgttcatat gcacataatc ccaagaaaat ataattttgg aggatttgaa
    45541 cttggaacaa atatgtatgt ttcttcaatg agccctgaag atttaacaaa aaagttaaaa
    45601 attgattaaa acatatattt ttaggtatta agtaaatagt aattttagat gaaattatag
    45661 tatagcattg ttaaaataaa agtaaattaa gaatttacaa taaaattgtt gaaaagcccc
    45721 catctttagc tatggggagt gtgtcaatgt ttagatgagg agtataagta tgaaatttat
    45781 atcatggaat gttaatggaa taagagcttg tgttgggaaa ggatttttag actttttcaa
    45841 agaagtagat gcagatatat tttgtttaca agaaactaaa ttgcaagaag ggcaaataga
    45901 gttagattta ccaggatatt ttcaatattg gaattatgct gagagaaagg gttattcagg
    45961 tactgctata tttacaaaaa aagagccttt aaaagtaatg tatggaataa atatagaaga
    46021 acatgacaaa gagggtagag tgataactct agagtttgaa gatttttact ttgttacagt
    46081 atatacgcca aattctcaat ctgaattaaa gagactagaa tatagaacta gatgggaaga
    46141 cgactttata gattatttaa ccaaactaga taatcataag cctgtaattg tgtgtggaga
    46201 tatgaacgtt gcacataaag aaatagattt aaaaaatcct aaaaataata tgaaaaatgc
    46261 aggttttact aaagaagaaa gagagaagtt ttctaaatta ttagacagtg ggtttataga
    46321 cacatataga tattttaatc ctgataaaga aggtgtttat agttggtggt cgtatagatt
    46381 taatgcaaga aaaaataatg cagggtggag aatagattat ttttgtgcat ccaaaaaatt
    46441 agaagataga ttagtaagtg cggatataca cactgaaata ttaggttcag accattgtcc
    46501 agttgaatta gagataaaat aaaaattaat tatgataaaa attgggctgt agatataaat
    46561 ttaaagttaa ctagtttttt aatctacatg cctatttttt atctaaataa actctatttg
    46621 ttttttcttt gcttaacatt ttctaatgta aattgatttg ttttccacat attataagct
    46681 aagttaatga tttttttctc gtctttagtt aattcacgat ttaaaatttt cctaaattca
    46741 tcaaataact tatcataatc aactcttttt ttgttagaat atcttatagt tttaggtttt
    46801 atactcgtat aaagatatat atctgggact atacttggat tgtatatctt aataaaaact
    46861 ttagcagtat aatatgcatc atttaaagca ttgtgataag acattttttc atcaagttcc
    46921 aatatagaaa tagcattttg taggccaatg ctttttcctg ctggattatt gaaatattta
    46981 gatgcatgtt gttgtatatt aatataagat ttaggtaagg aatttgatgg tagcttgtga
    47041 tagttgatat tcctgtataa ttccttcaaa tctccagctc cccaaacaca taatataggg
    47101 tcttgtgatg atataaattt tataaaattg ttgtagacct cagggaaagt aggcgaatca
    47161 ttaaaatcag aacttttaat attagtcata cgactaataa agggatgtat gtctttataa
    47221 attgtaggtt ttacatagct attaaacgta tctattatat tgaatttaga atccaattta
    47281 attgctccta tttgtataat ttcaaaagga cacttttcat tggatacagt tttattgagt
    47341 tttttatcaa atccttggtt aaattccaaa tcgaaaatta tgtattccat aaagtttatc
    47401 tcctaaattt ttttattatt tcttttacta tagattatat tataaataaa attaaatttt
    47461 caaataaaaa tacttttatt ttagtatacc ccatatttgt aaaaatgtta aactattgta
    47521 tgtataatat tttttccaat aaaaaatatt gcgaactttt tatacttgat atataataat
    47581 ataaataaaa acacgaataa aacacgaata atttgcaaga aaaattcata aatgtaagga
    47641 aaatgaaatt tatcttgtat ttaacaaaat aaaatataca taaaaattat gggagagtgg
    47701 aaaaatggat agtctattat acgtaataga gaaagataaa cacactaatg aagaattaag
    47761 agaaatccta aaaagcaata aaaatataag atttgtttcc ttaatggggg ttgatttagg
    47821 aggaaatgca acagatgaaa agattcctgt agaattattt ttggaggata tagataaatt
    47881 tttggaatca gctatacaga ctgatggttc aagtgttgag ctttataata tagctacatt
    47941 aaataatgcg aaagtagatt taatgccaga taaaagttgt cattggtatg tagattacaa
    48001 tatggaatat atagacgaag aagtaggtct acctgttgga acattaaaga tacctgcatt
    48061 tttaatacat gataataaaa aagtatgttc tagaggagta cttcaaaaag cagataagta
    48121 ctttaaaaag tcaatgtatg aaatatttag agaatatcca catgtaataa ataacatagg
    48181 aatagattca gtagatgata tagaggaaat aatgttaaca gcagctacag aacttgagtt
    48241 ctgggttaat actcctgagg ataaagctga cttagagaaa ttatatgtat cacaaagcct
    48301 aaaagagcaa tactggaaaa gaactcatgg tattataaga acttgtttag agaaaagttt
    48361 aattatactt caaaagctag gtgtaaaccc tgaaatggct cacaaagagg tcggaggaat
    48421 acaaagttct attagtatag atggaagaac aaaccatgct atggagcaat tagaggtttc
    48481 ttggaagttt tctacaccgt tacaagcagc agacaatgaa ttattagtaa gagatgttat
    48541 agaggatgta tttacatcac atggactaga agttacattt aaagcaaaac caattcatgg
    48601 agtagcagga agtggtggac atacacatgt aggagtaagt gcgaagttaa aggatggaag
    48661 tataaaaaat ctatttgcgc caaaagattt aaaagaagat tatctaagtg aactaggata
    48721 tggggctctt atgggacttc tttacaatta tgaagtatta aacccaattg taacagcttc
    48781 taatgatgga tttaatagat tagttcctgg atttgaagct cctgtatgca tagttacatc
    48841 tttaggtcat agttatgaga ttccatcaag aaataggtca gtgcttgttg gattaataag
    48901 agatatgaaa aatccaaaga ctgtaagatt tgagttaagg tcaccaagcc ctctatctaa
    48961 cacgtattta gtaatagcag gatgttatca aacaatgttg gatggtataa aagcagcagc
    49021 taagagtggt cttagtacaa aagaacttga aaaagagtta tctaaaaatg taggtgaaga
    49081 aagtttctat ttagaaaaag atagagcata tagagatgaa aatgatgtat ttgaacatta
    49141 tagtttggaa gaaagaaatg caagatttgg aataccaccg gctacagttt atgaaaatat
    49201 gaaaaactta gaaatatatg cttcaaaatt aaaatcttta aaacaaggtg atgtatttac
    49261 tgatagtata atagaatcat ttaagattgg agctatagat aagtggcaaa agaaattaaa
    49321 aactagaata atagaggctg ggattcaaaa aataagaagt attgtcaaga tacacacaaa
    49381 agaaaatatg gatgcgcttg atgaggtagt ttggaactca atatcggatt taaagttcaa
    49441 tattatgaag gatactttaa ctagagaatc actattcact agagtgagag aagctataga
    49501 aaataaggat taccagactg cttcagattt acaaatagaa ttaaagagaa gtatggaaga
    49561 aatacaacaa ctgtatatgc agtacaagaa aaatatatac taatataaca atttgatttt
    49621 gatatgataa aggcaatcaa gtattattaa acactatgag taaattctca agtgtaaaag
    49681 catacttgat tgcctttaat tttatgtttg aaatttttta atagtacaat ttaactgtaa
    49741 tgattatcta ttaattctga tatataatca gcaattttct ctggatgaat gactgtttta
    49801 aattctttta aatttttttt cataaagttt attatagaat caaattcatt tgatactaat
    49861 ttataaaata tgtttttaag gtctaggtca tcactataaa tcatgccaag acccttatct
    49921 attataaatt tagcgttttc aatttcttgt ccaacttttg gcatttttac gagtactgga
    49981 gtttgtgatt gaatagcttc aaatatagtt gctcctccag gcttagttag cattaggtca
    50041 cattctctta ttagattata catgtcgttt acaaatccaa gtactttaat attttttaga
    50101 ggttttttat gagttagatt gtcatataat tttttgtttt taccagtaac tattgtaatt
    50161 tctatagaat tactatgttc ttcaataaat tcatctatcc aatgcataaa atcctcatct
    50221 atatcaaata gacctcttcc tccacctaga agtaatagtc tatatttatc acaacatagt
    50281 tctttacttt caatttgaaa tctcttatca acaggaactc cagtaacctt tatggagtct
    50341 ggatttattc ctttctgaaa aaatctattt tttatttcag gagaaggaac aaaatacaaa
    50401 tctgtatttt caaaaaccca ttccatacta tctacaacat ctgttataac tgtaagtgta
    50461 ggtatattta tatcaggatt ttttatctta aagttattta cacaagcagc agctagtgga
    50521 aatgtagaga taatcaaatc tggatttata tcagcaatat actcagaaag tttaggtgta
    50581 tataatttat gagataaatc atgtcttgaa tcaaacgatt tcttaaagta ataatagtaa
    50641 ttatataatc caggagtata ttttgtattg ttttcataaa gttttaccat aggcttattc
    50701 atcatgggaa tacttgcatt tataaagttt tgtataacaa cattatagtt actatatttt
    50761 tcaataatac attctttgac tgcttttgca gcacttatat gtccagcacc aaattgagca
    50821 gttaggatta atattgtttt atcatctttc attttagcca atttatcacc actttcataa
    50881 aatatatctg attagagtat tatacattta tagaaggatt taatcaacaa gaatatttaa
    50941 aaataactat tgtgcagaac atagtactgt gttattatga ggatataaga agttttaaca
    51001 tagtaccttg taaaacacaa tactgtgtat ttaaagaaag gatggagtat atgaacacac
    51061 aatttagaaa gggtgtttta gaaatttgtg tacttgctct catatctaag aaagatatgt
    51121 atggatatga gatagtacat aacatctcta aagtaataga tgtaaatgaa ggaacaatat
    51181 atcctattct aagaagactt acaaaagatt tgtatttcga aacatatata ctagaatcta
    51241 atgaaggtcc tgctagaaaa tactatagaa taacttcact aggaaaagag aatttatcca
    51301 atctgatgga agagtggaga gaatttgtaa aggctgttga gattttaata agtgaagatg
    51361 agggaggtaa tgaacttgaa taaaactgag tttcttgaaa ttttaaagga ttatctaaaa
    51421 aaagattttt ctgagagcga agttaatgat atattgagag attatgaaga gtatttcgta
    51481 gatggtctta tagagggaaa gagtgatatg gagataatag catcacttgg ctcaccaaaa
    51541 tctatagcaa aagaacttat cttacaaact aaagaaaaaa atattgaaga ggaaaaaata
    51601 ataaatacag atgttttgaa tgataagttt agtaaattta aatcagatat gaaaaaaggt
    51661 tactctatct taaaagatga agcaaaagaa aagtatagta aatcaaaaga aaatatagat
    51721 ggaaaaataa tgaatacaga catattaaat ggtaaattta gtagatttaa atcaaatgta
    51781 aaaaaggatt attctatctt gaaagatgaa gctaaggaaa agtacaataa atcaaaagaa
    51841 aatataaatg ataaacttac accaagtata gaagaaggtg taggaggatt atccagacaa
    51901 gttataaagg ggttattagt agtgttatct ttatttctaa ttgttccagc atttgcgttt
    51961 gtatgtgcta ttgtatctgc tactattgca ttagtatgct cattaattgc ttttgttata
    52021 ttaattccat ttgtgattaa atttatagca atggttccag atgttatgat gttttgtatt
    52081 ttcgcaacca tagcatttgt agggcttgag atacttgcat ggcaaatttt attatttgcc
    52141 ataaaactag gtaaacaatt actaaataat tatatacatt ggataaagag aaaaaatatt
    52201 tatataaagg caaatgaaaa gatggaaaaa agaaaaaaag ctactttaaa tgatgaagat
    52261 ttgaataaag acattaatga aaattcatat aataatgatt actacaatag agaagaagaa
    52321 agcataaaag aaactgatga atataatgat gatagtataa attttgaggc gatatataag
    52381 gaccttgaag atatagaaaa taagaaagga gaagataaac atgaataaaa aacttgctat
    52441 atttgctgca gttttattgg taataggtat aatagggaca acatggtcag ggattttagt
    52501 tatgccaagc ctaataaact ttggtttaga aaaggaagct gaattaaaca aagaaaacaa
    52561 actttatcaa gaaaaagtaa atattgacaa gttagatata gctgttgaca atataaatgt
    52621 tatgataaga aaaagttcaa atgaagatgt gcgtgtgaca acaagaggga ataatgaatt
    52681 ttacaaatac agtgtaaccc tacaagataa gactttagtt gtaaaaggcg aaagaaaatc
    52741 tgaaaataaa attaaaaaga ttagaagttt cgaccactta ttaaatacat ctataaatag
    52801 tatgttttca cgtgattata gagaaattag aatatatgta ccaaataatg tagatataaa
    52861 tgcatcaagt ataagttcgt acttatttat atctgatgat gtagattcaa ataatataac
    52921 atataaaact tcttctggag gttttagcag agctataaca gaaaataaag taaatagatt
    52981 agaaaactta aacttaatat ctaatagcaa tcttcatcta tcaacaaagt caattttagg
    53041 cgttaaaaat gtaaacatag aatcagagtc attggatata tcttcagaga atggagatgt
    53101 atttatcaat aatatagaag agtatatacc tgaaaatgta aatataaaag agaaaatagg
    53161 tagaaattca aattatgaaa gtgaatttta cttgtactct gatatgccaa ttgctaagtt
    53221 cctggacata gaagttccaa attctaaggt aagacttgat ataccagtaa ataagtataa
    53281 atttaattgt gatataaagt caaaagaagc aattgaagaa tttaataatg atgaagaata
    53341 tgacaatgat tcatatgatt atgaacataa tgaagaacat agcaacaagt atagaaatac
    53401 gagggaaata aaagggcttt tgaataaaaa tctgtcgaat ttagagaaag aatatactat
    53461 aaaaataaat tcaaatagct tagaattgta aataatttac tataataatt tataaattga
    53521 tttaaaagta taaaaatata atctatacta tggtataata agtatcgaaa gagaattagg
    53581 agagggatac ttatgaataa aatagcagta agttttttaa taattgctac tactttattg
    53641 agtacggcat gtatggatta tagcatcagt gcagttgagc tagttgattc taaagaatca
    53701 gcagttgtaa agaaggatga agatgcaaaa gaagaaacaa ctagtaaaat gattaattct
    53761 aaaaaaacta ctaaaatacc tatagagata atttcaaagg atgagaaaat agtaaagtac
    53821 ttacagattg atgaagaaag ttcacttaag gataaactgc gattaatctt agatacatta
    53881 tcaaatgagt attttaatgg tttagacatg gaagtagaag ttaaggaaaa agataatctt
    53941 gttaaaataa acttgataga accagataaa aaaagtagag tttcttggaa agatgattat
    54001 ttgaatgaac aaaatataat atatactata aataatatta ttaaaaatgt catccaagaa
    54061 gaagataata gcatttggat tgaagaagta gagatatact acaatggaaa attaatagaa
    54121 ttaagataaa aaaagatgtg tttttaacac atcttttttt gttttttaat tcttataaaa
    54181 tccttatatt tatgatttag aatgattctt gtaaataagt ttgacaaaag aaaggatgaa
    54241 aattatgagt aattatattt tgcaggtaaa aggagtttct aaaaaattta aggagcagat
    54301 ggcagttgat aacatttcac tagcaattaa atctaattct atatatggat tattaggacc
    54361 aaatggagct gggaaatcta ctttattaaa aatgataact gggattttaa atccaacttc
    54421 aggaaaaata atgtttgagg gacatagatg gactagaaag gatttatcaa atattggttc
    54481 tcttattgag tctcctgcta tatatgagaa tttaacagca cgagaaaatt taaaagtcca
    54541 tactacttta cttggtcttc ctgactctcg cattgaagaa gttttagaga ctgtagattt
    54601 aagagatacc tttaaaaagc gttcaggtca attctctcta ggcatgaagc aacgccttgg
    54661 tattgctatt gcattattaa atcacccaaa acttttaatc ttagatgagc ctacaaatgg
    54721 tcttgaccct attggtattg aagaacttag agaattgatt cgttcttttc caaaacaagg
    54781 aattacagtt attttatcaa gccatatctt gtctgaagtt gaacaagttg tagatgaaat
    54841 aggaataatt tctaatggag taatgggtta tcaaggagaa gtatcaaaag aacaaaactt
    54901 agaagaactt tttatgaaag ttgtagctga aaatagaaag agagggcagt aatatggaaa
    54961 gatttattag agtggaattt caaaagatga aaggaacttt tggaatgaag ttgatatgga
    55021 tagttccagt gttttgtata cttttgtgtg gactaggtgg agggcaaaat gctgctttca
    55081 actggtggta tacgacattt cttccaggag cattaacatt aatatcttct ttaatagtat
    55141 taaaagatga aaaaagtaaa tatcgtggta ttttaccatt gccaattgaa atgaaagacg
    55201 tttggactgg aaaaatgatt tactgtatta tttgttgttt tatagcatca attatttctg
    55261 caataggtgt agtttttgtt gggataatct ataaagaaac tatttcaata ggtcaaaata
    55321 tcattgcaag tatattaatt attttactat ttatgtggca gattcctctt tgtatgtttt
    55381 tagcttcaaa atttggtatg tttgttacag ttctaacaaa tatgttcttt actattgttg
    55441 gagttgctgt tttctcaact acagaaatgt ggtatatgtt tccatattcg attccttcaa
    55501 gattaatggc tgttgtattg catattctgc caaatggtct tcctgtacca gagggaagtg
    55561 aatttttaaa taatgctgtt attttgccag gagtattaat atctattgtt ttatttgtat
    55621 tatttgcttt agctacaagg gtttggttca ataatagaga ggtgaagtaa tatgagacag
    55681 ttaataagaa tattttgttc tgatttaata aaattaaaga gaaattttat aatactaatg
    55741 cacatttgca ttgctttaat ggggatggga ttgtttttat ggtattataa ggtttcagga
    55801 gctgataata tactaaagat agtagcatat ttacaagtta tagcaatagc ttttccttta
    55861 ttgtcaagtg ttgtgtgttc aatgtttgtt gaacaagaat attatgcagg gaattataaa
    55921 catatgttaa caacatctaa tccaaaatat cttactctta taagtaaata tataatctta
    55981 gtatgtcttg gttttgtagc aacattagtg tctgtattag gttttaaatt tggtatatct
    56041 aaggcttact ttacatcaag cttttatatg atttcaattc taattttaat tgggtgtaat
    56101 ttatttgaat acatattaca tttattctta agtttaaggt tcggaaaagg aacatcaatt
    56161 ggagtaggta tagttgaaat gttactttca gcattattat taactggatt gggttctggt
    56221 atttggcagt attttccttg tgcatggggt gttagattta taactatttg gtcgtctttt
    56281 tcaagttcta aaacggttga atatataaag gttgaatcaa taaaagcata tcaatcaaca
    56341 ggtttaattt gtggttttgt aacaatttta gcatttgtaa ttttatgtat ttggttttct
    56401 aaatgggaag gtaaaaaatc agaagaatag agtaaaaatt gactgtttat ttatattatt
    56461 atgttaaaat ataacttagc aataaaaaga tataaaataa tcaaataaaa aactggttgg
    56521 gtattaatta tgaattaatt aaactattgg aggacagggt acaaatgctt ggaggtaaaa
    56581 aatctaacaa caaaaaagtt gaaaagttaa gaactatttt tataaaatat ttatctttat
    56641 tctttattat gacaatttca attgtactcc ttttaatgtt atcattttct gtattattat
    56701 catcaggtgt aattttacca gctaattatg cagaaaaaca atttaataaa tataaagaac
    56761 agattatttc aagtgaaaaa gtaactgaag atataattcc aagtatatat gagtatgggg
    56821 tttatacttt ggatgggaat ttaatttcag ggacctttaa taaaaaagaa tcaaaagaag
    56881 tatggaattt aatgagagat attgaagaaa ggcatgcata ttcagaaagt tatataaagt
    56941 tttttaagaa agatgaagtt tttataataa aatacaaaat agtatcagag tatagttctc
    57001 caattttaag agcatattta ccaaaaccag agacattggg tatgattatt tttagtataa
    57061 tattttttat tgaaattgtt atattatcaa aagtatttgg caagaaattt aatattgaaa
    57121 tggagctttt aaaaaatact acagaaaaaa tagaacaaca ggatttagat tttgttgtag
    57181 aatctagtaa aatttatgaa attaataatg tccttttttc tatggataaa atgaagttgg
    57241 cgttaaaaga ttctttagaa aaacaatgga tgctagaaga aaatagaaag gaacagatat
    57301 ctgcactggc acatgatata aaaacaccac taacaattat acatggaaat actgacttat
    57361 taattgaaat aaatgaaaat cctgagttat tagaatacat ggaatatatc tcaaagggtg
    57421 tcactcaaat agaaaaatat attaatactt taatagatat atcaaaaaca gagaatggct
    57481 atatattaaa taaagaagca atcagtatga gtgagtttat aaaagatgtt ttagtgcaaa
    57541 tagaagcatt agctagtact aaaaacttag atgtggagtt ttataaacaa gataatttgc
    57601 ctaaaaatat aactatagat agagaactgt tattcagagc tattatgaat ataatttcaa
    57661 atgctataga ttattcacca agccagagta aactatacat aagtgtatca gtacataata
    57721 aatacttaaa atttgtagtt acagattgtg gaagtggttt ttctaaagca gatttgtcaa
    57781 aagctacaga acagttttat acaggagatt tgagtagaaa ttctaaatct cattatggaa
    57841 tgggactcta tattgtaaat aatattgtac aaaaacataa tgggacttta tgtattgaaa
    57901 attctgtgaa aactggtggt gctatggtaa caattgaaat accaatagtt tagtaaataa
    57961 tatccatttt gtaaatacct aaattttatg atttataggt atttataaaa tggatagttt
    58021 ttttgatttt atatattggt taaatctgat aaaaagtttt caaacataac tccatcattt
    58081 ctatcagtat caaattgaga tgtgtaattt atagttttat atataaaatc tgttgcagta
    58141 ttaaccgcta ctcccaaatc atgtttattt actaacatac cacaaagtat agatgtgaat
    58201 atatctccag ttccactgta agaacaatta ttatatttga ctgatgtaaa gaatacatgg
    58261 tcattatctc tattgtaagc caaatttaat atattatcat cttctaatat accagtaata
    58321 acgactttgc taggtcccaa atcagataca gattttgcaa tatatataag ctcatctcta
    58381 ttataatcgc ttttggtata atcatttcca gttaagaaac aagcttctgt taaattagga
    58441 gtagttaaat ctgaatgttt aactaattct ttaatttcct gtctcatttc ttctgtgaat
    58501 attggataca taactccatt atcacccatc acagggtcaa ctacgataaa agcattggga
    58561 taatcgttta taaaattagc cactatctca atttgatgct ttgaacccaa aaaaccacta
    58621 tatatactat caaaattgac ttttaaattt ttccaaacat ttgaatattt taccatttca
    58681 tcagtaaagt ctaagaaagt aaattcagga taaccagttt gacttgaaag tatggccgtt
    58741 ggaaatggac aacattgaac ctttaatgct gaaagtatag gtatagcaac cgataatgag
    58801 cattttccta ttccagataa gtcattaatt gctgcgactt ttttttgcat aaaaaaccct
    58861 ccattagtat gtattaatta taatgatacc aaaatattta tatacatata tattactata
    58921 aaaaaaagaa aaaagtagat tataaaataa aaaattgtaa aaaaaacagt gtattgatac
    58981 aattaatttt taatattgga cataataaaa tagagaatta ttaaatatga aaattactat
    59041 atgtgacaga atattctttg taatttgatt tgtaaggtgt actatttggg aggtgtagct
    59101 ttgaaaatac tgaaaaataa gaataagtac atcaaaggta taatggcatt tgtatttttg
    59161 tctttagcta ctacagcagg tgtattttta atcaatgatt taagtatgaa aaatatggaa
    59221 gaaaaagtca tggaatctgc aaatgttgaa ggtgatgatg taaaagacca atataaacta
    59281 ttgcttgaaa acttatttga ctacagaaat aaggcaatat tagagaaaaa tgaaaatatt
    59341 ttaaaaggtc tttatgaaac ggataaaaaa tttggtctgt gggcttatga aaatgaagta
    59401 aaaaagatga aatatttaga aaactggtct agtaaacaag gtgtaaaatt taaagacata
    59461 aagacaaggg ttaagattaa aaaaataaag gaaaaagaaa aagacctcta tggtataatc
    59521 tgttcagtat ccactgaata taagtattca tatgaaaatc aaaaggacaa agaaaatgcc
    59581 tttagaatag gtacatatca ttatctaaat gtaaaaatta tagataatca atatgtcata
    59641 actaaagagt ggtatacaga cccttttgca gactctttaa atttagaaaa tataaaatct
    59701 gatgatataa gaaattatat tggtcaacaa gatggtgtag agttacagct tacagatgaa
    59761 caaaaaaaag ctatagatta tgcacataaa tactgtggag ccgcagctga tgaagaacat
    59821 ggtatgaaat ttaatccaaa ctatagggat tataatccag aaggtggaga ttgtgcaaac
    59881 tttgcatcac agataatgtt tgaaagtgga aggttcaaaa agaactcaat ttggaattat
    59941 agcgagagag caggtacaaa agcatgggta aatgcacaag cttttaaaaa ttatgcactg
    60001 tatagtggaa gaggttcatt aattgctaaa ggttcctatg aagaagtata caaagaagct
    60061 tataatttac gtccagggga ttttgttggt tatgagaaga aaggcagaat aacacatgtt
    60121 tcgacagtga caggattaga ctcaagagga tatcctttgg taacttgcca taatactgat
    60181 agaatgttgg tgccatggga tttaggctgg agtgataagt ccataagatt ccatataata
    60241 aaaataaatt attaaaaaat tataaagaaa aataaagtgt aaaagataat aatcatttaa
    60301 aattataaag taaaaaaatt gattattaat gtataaaaat aataataatt gacaaacata
    60361 gctatatatg atagagtagt aataaggtta cagaaaagag tagctaaata tttgtgtttt
    60421 tttaggagga tttattaatg aaaactggtg tagttaaatg gtttaacaac gaaaaaggat
    60481 ttggattcat atctgtagaa ggtgaaggag atgttttcgt acatttctca gctataactg
    60541 gagatggata caaaacatta gaagaaggac aaagcgttga gttcgaagta gtagatggag
    60601 ctaaaggtcc tcaagctgct aacgtagtta gattataatt aaaaattcat atatattagt
    60661 gttctagtaa tatgagagat aagccgctaa tcatttagcg gttttttcag tttttgtaaa
    60721 taaaattaat agcaaaataa attaaaatta ttaaaataaa atcattaata atgagataaa
    60781 tatgttaaaa ttaatagtgt agagtaaaat tattatggct aagtttaagt caattaattt
    60841 ttagtaaatt ttctaaaaag agaataagtg atacttatag accatataaa aatattaata
    60901 aagcataata agattaaaat gagcaaagga aaaacgcttg ttacagcgta tagtattaag
    60961 aatacttaga aaataagaaa ggagaaagtt actgcaaaaa agtaacatta taaatattga
    61021 gtaatattta taagttttca gtaacggtgg gtagtgtaat gaacatatta ttctttctaa
    61081 caccaaaaag tgaagtagca tatatatatg aagactacac tataaggcaa gccctagaaa
    61141 aaatggaata tcacaaatac tctgcaattc caataataag taaggatggt aaatatgttg
    61201 gaactataac agaaggtgat tttttatgga cattaaaaaa tgatttaaat ctggacttaa
    61261 agggattgga agatgttcca gtgacagata taaatagaaa aatggataat tctcctgttt
    61321 ccatcaacgc agatattgaa gatttagtta taaaatcttt aaatcaaaat ttcattcctg
    61381 ttatagatga ccaagacaca tttattggga taataaaaag aagagatgta ataggttact
    61441 gttatgagat aatacgtgga tataaaaatt tagctaatga taattaaaaa tcttatttaa
    61501 acttttaagc tgagttcttt acaataaaaa aatgataata tgaaactgaa tatttaaact
    61561 ttaatataaa aagctgtctt ttctaataaa tattgtgaaa agacagcttt tttatatatg
    61621 accttatgat taatcgttgt attttacaag gtttccgtac ttagcattaa gagcatttat
    61681 cttatttcca taaacttcat tttgtttttg atataataaa cttttttcaa cttcagcctt
    61741 aacttcatca aatgtagact ctgtactttc ttgaaggtct tctaatttta ttatatggta
    61801 tccaaattga gttttaactg gttcacttac ttcaccttta gccatagaga acacagcttc
    61861 ttcaaattca ggaaccattt gtcctctacc gaatgttcct aaatcaccac ccatatcttt
    61921 agatgggcaa gaagaatgtt taagagctgc atcttcaaaa gatatttctt ctgattttat
    61981 ttgagcaagt atttctttag ctttttcatc actatctact aatatatgtt tagcagttgc
    62041 actttctggt ttagagaaag agctcttatt agcttcaaag aattttactt tttcatcttc
    62101 tgttacatta acactagata aaactttatt tatagcaaat tgctttaata cattttcttc
    62161 tatctttttc atttctaatt taaatatttc ttcttcatct aatttttctt ctttagcatc
    62221 taagaagaat aattcttgat tcactaaatc atttaataag tgtttttttc cttcttcagt
    62281 tttaaattgc atagcttgat aagggtctaa tgattttaat gcattttcaa tatcaatatt
    62341 agttatctct ttttctccaa ctgttgctaa aacttttttt tccataatag ttaactcctt
    62401 catagtgtat ttcattatta atataagact accatcttaa ataatatcag aaaataatgt
    62461 agatgtctaa tatttaaagc catattaagt caaatagaat taaatttagt agaacaaagt
    62521 tattacaatt aataaaagtg agctcataat acccactaca ccttcgtcat ctttaaattt
    62581 aagagttttt actattgaat aataaaggta tattccagta aaaataatct ttaaatacaa
    62641 gaaaaataaa ctctttgggt atattaaaaa gctaaaaagt gtaaatataa aagcaagtag
    62701 agtacttatc ctgtaaaaga aatctatttg tgtaatgtct ttatatttta tcataaacac
    62761 cgaatataaa atgatgaaaa caattgcgac gattgcatat atggtaaata acatattttc
    62821 aagagtacca tttgaaatta ctatccactt ataccacata attggccaaa ataatagtgc
    62881 taagaactta aaataattat caaacaactt ttctttatac attcatcaaa caacctttct
    62941 taacaaaagc atatatttgt ttttagaatt ttaaataata tgatatcatt attatatatt
    63001 aatattgaat ttatagaaac caaaatttgt taaaataaat atatagattt tactgttaag
    63061 ccagttaaaa ttactactat ttttattatg aaattggatc aaatatgtag aaatacggca
    63121 aattagttaa tattaaatat ttattatttc caagttgtaa agactgtttt tttaatgata
    63181 gaaattctaa tcttttttga aagaaagtaa tatccacatt aagtatgtct gccatttcat
    63241 aaacgcaagt gatgccagag ttaattatgt ttattatatc ttcttcagta attaagaact
    63301 cacaagccca ttttaaggct ttattttcgc acttatctat aataattttt gtataataat
    63361 cgttataaga ggatacatag tatccaaggc tagtgaaatg atgtccaagt tcttcagcta
    63421 agatggatgt caattttttt gagttttgtt ttaaattact gagtaatgat ataattttaa
    63481 taccatgttt gtttatatat agtccttcta aatcacctgc aatataagtg gtataatgaa
    63541 ttattatctc ttcttgagaa gctaattcaa aaagcttatc caaattattc ataaaaatcc
    63601 ccctaaaata gaatgtatgt ttgcctttga attatattaa aagagcagaa aaatagactg
    63661 ctcatcatat ggtttatttt tttttatatt tatttagtaa aaattctata taatcattaa
    63721 gttgttcttg tgcttcttca ggtaactctt catgtggatt ttttctatgt gcagctactg
    63781 tatcaatatt ttccttaact aaggttcttc caagaaggta atcaactgat acattaaata
    63841 catcagccaa tttatttaaa atgtgttcat caggaaatct gttttctgtt tcatagtacc
    63901 ctaagactct ttgggaaacg cctacttttt ctccaagttc tctttgagtc aatccaaatt
    63961 cctttctaag ttctcttaat cttttggcaa acattataac accaccttat gtatagatta
    64021 taacaaattg ttctaaaaaa taaaactaat aaaatataaa agaatatttt tttctaaaat
    64081 ctattgataa agaacaaata attctatata atctaagtga ggaagaacaa aatattctta
    64141 atagtaatgg aggtataaaa caatgtttaa aaataacttg aaatattata gaaaatgcaa
    64201 aggtatgaca caaattcaac ttgccagaaa ggctggaatt acaaatgatt atatatctca
    64261 aatagaaaga ggcataaaaa atcctggact tcttatggct aagaagattt ctagtatttt
    64321 agaacaaaat atagaagaag ttttttttat acagttatag aacaatatgt tcttgaaggt
    64381 tgtgagatta gtaaaaaact gtgcactaaa gagattattg taaatttgaa gctaataata
    64441 agtatataaa aaaggggaag tactatggaa aacaaaaaag atatattatt taaagaaaca
    64501 gataaaagat tacataatta taagtatttg gatataaaga taaagaatat taacttggac
    64561 ataaaaagat gtgagaatga atactctgga tgtggagcaa tggtatatac agaaaagact
    64621 agtaacacat ataacataag ctcttctgtg gaaaatgagg tgttaaaaag agaggaaaga
    64681 ttaagaaaat taaaaatgga aaaagaagat atagaaatag aaaaagagaa gatagaaaat
    64741 gctctaacgt gtctaaatga tatagaaatg gaatttttta atctttttta taatagtaag
    64801 acaaaaaaca atatgacata tatttctatg aaactacact tagatagaac atcttgctac
    64861 aatttaaaga aaaagatgat atttaaattg agtgagatat tataaaaaat aggacaattt
    64921 tacaacactt tatatacacc attacaacaa taggcaataa aatatgtgag ataatgttat
    64981 tgtgaaagaa atccatattg aaggaggtga tagattgaaa agaataatat tacctaaaaa
    65041 tatagaagat acttgacagg aataaatgag atatatattt aaaaatgact tatatcattt
    65101 atagtaagat tatcagatta agcaagaata tttagtgata gtgtggtgat tatttgctta
    65161 aatacaagga aatattagaa acaattattg agattctcaa aaaaaacttt actgaaagta
    65221 tttttattga tgatgaaagt gtgcaaggct ctgaagggtc ttgttttttt gtaagtatac
    65281 tatcagttat ttgtacacct gtaatgttaa atacgaataa caaagatatt gttatctcta
    65341 taaaatactt accaaaacca cagtcaaaga gtattagaat gtatgaagtt tcagatgaat
    65401 taaataagtt attcaacaga aatataaagg taacagacag aaaattaaat ataacaaagc
    65461 tagaacaaag tattaaaaaa gaagagtcaa tttatgtatt gaactttaca tttacactaa
    65521 actatctgga tagtgtatat gaagaagatg tagtatatga aaatatggaa gaaatcaatt
    65581 taaatttagg agagtgatag tatggctata ggattaccaa gtatcaacat atcatttaag
    65641 gagctagcta caactgttaa agaacgttca gctagaggaa taattgcaat ggtacttaaa
    65701 gatgctaagg cactaggtct taatgaaata catgaaaaag aggatatacc agttgattta
    65761 tctgctgaaa ataaagaata tataaattta gctttgatgg gaaatgttaa cactccaaat
    65821 aagttattag tttatgtaat agaaggagaa gcagatattc aaactgcatt agatttttta
    65881 gagactaagg aatttaatta tctatgtatg ccaaaagcag tagaagctga taagactgct
    65941 ataaaaaatt ggataattaa acttagagat atagataagg ttaaggttaa agctgtatta
    66001 ggaaaagttg taggaaatca tgaagggata attaatttta ctacagaaga tgtgttagtt
    66061 ggagaaaaga aatacagtgt tgatgagttt acaagtaggg tggctggact tatagcaggt
    66121 acacctttaa gtcaatcagt aacttatact aaacttagtg atgtagtcga tatacctaag
    66181 atgacgaaag ttgatgcaga atcaaaggtt aataaaggag agcttatact tattaaagaa
    66241 gcaggagcta taagaattgc tagaggagta aattctttaa ctgaattaac agcagaaaaa
    66301 ggagaaatgt tccagaaaat aaaaatagtt gacactttag atattataca tagtgacata
    66361 agaaaggtga taatagatga ctatatagga aaggttacta acagttatga caacaaatgt
    66421 ttattgatag tagctataaa aagttattta gaagaattag aaaaatcagc acttatagaa
    66481 tctgattcta ctgttgaaat agattttgaa gcacaaaaat cgtatttaaa atcaaaagga
    66541 gtagatttat cttatatgac attacaagaa ataaaagaag ctaacacagg ttctaaagta
    66601 tttttaaaag caaaaataaa agtacttgat gctatggaag atatagattt atcaatagaa
    66661 atataggagg attattaata tggcaaatat ggaagctaga aatgtaatga gtggtacttg
    66721 gggagaactt tggcttgatg gaaacaaagt agcagaagta aagaaatttc aagcaaagat
    66781 ggaatttaca aaagaagata ttataatagc aggtcaaatg ggtactgata caaagtatat
    66841 aggatataaa ggaaaaggct caataactct ataccacgtt agttcaagaa tgcacaagtt
    66901 aattggagaa aagataaaga gaggttctga acctaggttt gttgctatat ctaaattaaa
    66961 tgacccagat tcttatggag cagaaagaat agcagtaaag aatatagcat ttgatgattt
    67021 aactttagct gattgggagg ttggcgtaaa aggagagata gaagcacctt tcacatttac
    67081 tgagtatgat tttcttgata taatttagtt ttatatttag ttttatattt ggttttatac
    67141 tgatatttag taaatatata cttaataaat tcagatagtt aataagtaaa aaagttagtt
    67201 gattgaattt gattgataaa ggagcaaata ataatgagtg aaaatggatt atcaaaaaat
    67261 ataaacatag tagatttact tttaaattca gatacagaaa acttagaaag accgagtact
    67321 atagttgaac ttaagagatt atcaactata tttgggcagg aatttaaagt aatgtgtaga
    67381 gctttaacaa taagtaaaga tgaagaaata caaaatactt gtcttaaaat tgatgaaaat
    67441 atgaaaacgg atatagactt accggagatg cagatgctta caattataga aggtgtttgt
    67501 gatttggatg gaaagctttt atttaaaaat aaggagctaa tggataaatt taaggctcca
    67561 acaccaaaag aattagcaag aaaattatta ttaccaggtg aaattaccaa cttatataga
    67621 atacttcaag atgttatggg ttatggtaaa aatgcagtga tagaagaggt aaaaaactaa
    67681 tagggacgga taccaggact acaataatgt actattattg gaagaaaaaa ggtataagac
    67741 cgtccctttt ttatgcaatg gataaaggcg aattaaagct tattgaagct tttttcgcct
    67801 tagaaattga ggaagaagtt gaaaaaatga aacatggata tggagtgtgt cctttgacag
    67861 gaggtggtat gtaatgggaa atgtgagaga agaaggtata aatatgtacc ttacagataa
    67921 ttacacacca aaaatgaatc aaattatatc agtaactgat aattttagga gagcaactgt
    67981 ggctgtttca ctttccacta atgtaatggc tagtagcata aaaaattcta ttggaagtgc
    68041 aagtaataga gtaaacagtt taaattcctc gttaagaaaa gttcaaacta ctgctagtag
    68101 tgtaagttca actatgacaa aattaagttc tagcataaat gctgtttcag gagttattgg
    68161 aagtttaaat ggaagtatta tgatactagc aataactata gctatgatta ttgattattt
    68221 taataagttg attcaaaaga aaaatgagtt taattcaaat attatgatta tattaatatt
    68281 taaagctaaa agtgatgaag tagaaaaaac taaaaataaa ttacttggaa atttaaaaaa
    68341 gattggtggc aaggtttgga atatcgtaat aaaagcaaaa gatatgacta agagagttat
    68401 aagtagtatc ttgggaaaat taaaacaagt agagaaacgt ccttatcaag gaagtattaa
    68461 tcttaaagat atggtgagta gtgctatggg tagaattttg cctaagttaa tgttgtttaa
    68521 aaatactttt tggagtggtg taatagctat aaaagatatg gcaagtggca ttataagtaa
    68581 agtatttccc aaattgagat tatttgcagg taaggtatgg agtggtgcaa tagctgtaaa
    68641 ggatatggca agtggaatac ttggttcgat aaaagggaag atatctgatt tgacaaatgg
    68701 tgctactata ggtgtcgctg tgaaaaaggg tgttgattta cttggtcagg aacaaaatca
    68761 gaaagttgtt ctagaaagtg taatgaaaag aaatactgga aaagttaatc aaatagatgt
    68821 tgatgattat tatggcagtt tagtaagaat ggcaaatgat acgccttttg accctgaaga
    68881 tgttgttgca atgggaacta aagctaaaat gattagtaat attactggtg gcaaaaaaga
    68941 aaaagatata actcaagcta tggtagatgt tagagcttta aatatgaata caagtagtga
    69001 acaagatgta tcagcagctt tcttaagtgc agcaaaagga aacatggaat ctcttaatac
    69061 tctggtagga gaaaattata aaacttttga tgaagcattg gaaggcataa gtgtaaagca
    69121 gatggggtta gctaaagaaa tgagtaatac aataccaggt ataatatcag gagctcaaac
    69181 aagcattaac aatggcttga agagtattgt taaacctttt gatgatattt taggtcaagg
    69241 actaaagaaa ataaaaactt ttatagaaag tggattaggc aatttagctg gcttatctga
    69301 aaaaatggct ggtaaaatag gcaatgtaat gaatggtaag ataattattg gcaacaaata
    69361 tgaccagatg caatctagaa gtgtaaaaaa tggaaaagag ttttctgatt ctactcaata
    69421 tcgaatttct aatgaggctg aaaagcgtaa aatgatggtt gaaaataagc aagaacgttt
    69481 tgaaaatcat gcagcaacaa tgatagggaa tgcaccaaaa gcaattgtta acgcaggaag
    69541 tacactatta caaaatatcg attttacagc attaatagat tcattacttc cagtagtaaa
    69601 cttagtaaat aatttactag atagtataaa caataaatca ccaattgcac aaggattaat
    69661 aagtatattt ggtacaatag taactacagc attccaacta atcggacctg tagttgaagc
    69721 tgttagtcct attatcacaa gaatttttac ttttttaggt gaatatgcac ctcaaataaa
    69781 caattttata gagacactgg gtgttatttg gaaaactgta tgggagacct taggacctct
    69841 gttggaaact ggatggaaaa ttatagagcc aatattggga gcttttttta acatattaga
    69901 taaagtatgt aaaatagtta aagatatatg caagtggtgg caaactatga ttaataagat
    69961 aaaaaatgga agcatcacag gaacagtttt aaatctagtg gaaaagagta aaaaaaatta
    70021 caaagataat ccatatgctg gaacaaaggc tggtgattct ggtaaagctt attcaggtaa
    70081 gaaaggtaat aatgcatttg gattgaacta tgttccttat aatgactatc aaaccagact
    70141 ccatgaaggt gaaatggttt taactaaaca agaagcaaat caatatagaa gcagaaaaaa
    70201 tggtggaaat ataaacatag ctaagttagc tgatacaata gtgattagag aagaagctga
    70261 tatagaaaag ataacatcaa aattagttgc aagtatccaa ttggcacagt tagggggtgt
    70321 cttataatgg aaatgtggct tagacaagct gaagatagat ttagatttcc agtatttcca
    70381 tcttccttta gtattaatgg aaaagctgct gtaaactctt ctagtatact caaaataggt
    70441 gaaatagcaa cttttggtgg tgtagctctt aaaagcattt caatatcaag tttttttcca
    70501 aataaagact atactttctg tgactataca ggttttccat caccatatga ttgtgtaaat
    70561 aagatagaaa aatggatgaa ggaaggtttt atattaagat ttacaattac ggaaacaaat
    70621 ataaatatgg aagtcataat tgaagggttt agttatgaag aaagagatgg gactcgagat
    70681 gtatatttta cattagattt aaaagagtat aaaagaataa agataccaaa agtaactcca
    70741 aaacaataac tattatagat aataagttat aaataactgc ttatagaatt aaatgaaaag
    70801 gcaggtgatt ttttattatt aagatttggg tacacataaa aaacggaagt atatatgaca
    70861 taactgacat agtagacaag gtatcatggt caggtgatta taaatctcca tcaaggacac
    70921 tagagttttc aataatacaa tcatcatttg atgtaaattt ccaacaaata gatataccaa
    70981 tagctagtac agtctgtttc tatgtagatg agaaagaact ctttagagga atgataatta
    71041 ataggtctaa agattcaagc agtaatgaaa ttagttttgt atctaaagat atgggatttt
    71101 tacttacaca aagtgaagtg tcatacaatt ttaaagataa gttagttgaa gacatagcaa
    71161 agcaagtatt tgctgaaaat aggctttcag ttggaacaat agcaaagacc aatgtcaagt
    71221 atacaaagat gtttatagga gtaaatggtt atgacacaat aatgagtgca tatacagaag
    71281 caagtaaaaa gacaaagaaa aagtatatga tagaggccaa tttagataag tttaatgtta
    71341 ttgaaaaagg aactgttaca ttaaatgtta tgtttgaaga gggatttaat attataaata
    71401 ccaccttttc ggagagcatg gaaaatgtaa aaaataaagt aatagtggta gaccagtatg
    71461 gaagcaagat tagcgaaaaa atagataatg aaatttttaa ggaagtaaat gtaataatgc
    71521 aaaaagtaat tcagcaacaa gaaaatcaag atgtagatat tgatagcgag tttaatggga
    71581 tagaaaaaag ctgttctctt aaaggttatg gagatgtaag ttgtataact ggtagaggag
    71641 taaaagttaa agattcttat acaaagcttg taggactatt ttatatagat acagacaaac
    71701 atacttggca aaatggagaa tatcaaattg agcttgaact taattttcaa aatcttatgg
    71761 atgaaaagtc agcaggacag gatgaaccta aggaagaaag taacttaggg ggagaagatt
    71821 atgcaggagg aaaagagttt acagcagaat ttacagctta ctgtcctaga aaagaagaag
    71881 gtggagatac agattgtaga aagaaaaaac ttgacccatc taaaaaaact tgcgctgctc
    71941 ctatggttgg taaatatgaa caaacttatt atacaaaaga gtttttaaat aaacatcctt
    72001 tattaaacta tggagatgaa atacaggtaa ttacaggagt ttctggtcgt gatggagtct
    72061 ataaagtaaa tgacgtagga cctgcaataa ctatagaaaa gaatggaaca taccatatag
    72121 atattttatt tggaaatgtt gaagaagcta gtaaatttgg aagaagaaaa ggaaaaatta
    72181 ttattggtgg ttattctggt aatgtatctg ataaagctaa aatagtaata tcagaagcaa
    72241 aaaaacatct aggtaaacct tataaatggg gtggaaatgg accaagtagt tttgactgtt
    72301 ctggtttaat ggtctattgt tttaaaaaag ttaatgttag tttgccaaga acgtcaaatc
    72361 aacaatctaa aaaaggcaag aaagtagaac aaaaaaatct tcaagcagga gatttagtat
    72421 tttttcataa tccagtcagc catgttggat tatatatagg taatggagaa tttttacatg
    72481 ctccacaaaa aggtgatgta gttaaaataa gtaagttaag tagtagaaga gattttaata
    72541 cagctaggag agtattataa aaggatggtg atataatggc taatccaata aatgaattta
    72601 taggaataat aagagaagaa ggaaagtatc ataatcaacc ttcttttttt attggaaaaa
    72661 ttaaaagtaa attaccagat ttaaaaatag agacaaataa catcatatta gaaaaagaag
    72721 atattttgat agatagttgg atgattgata gacagctaga aacatttgac acagaaacaa
    72781 atcaagaaca ccagcatgaa gtaaaaaatc cttttataga taattttgaa tctggggata
    72841 tggtaataat gtttagaata ggcgaaaaat ttgctgttgt aagtaagttg gtgagcttat
    72901 aatgagtaca atatttcctt ttataggtgt cccagaggat tatatcttac ctaaaacaga
    72961 agaattgcca atctttcgtg aagtggcatg ggattttgaa aaagatgaac ctattttaga
    73021 aaaaggtgac tttaaaataa ttgaaaaaaa agaagcctta aaagtttgga tatacaagtg
    73081 tataaagaca aatagatatg aacatgagat atactcttta gaatatggga cagagctttc
    73141 agaactaata ggacaaaaat atacaaaagg tcttacagaa agtgaagcta gtagattcat
    73201 aaaagaggcc cttctaataa atccatatat attagaagta aacgtaaaaa gtgctaactt
    73261 taacagagac gtattgagtg caaatgtaaa agtatccact atctatgggg aggtggaaat
    73321 aaatgtatag tgaccagaca tatgaagtaa taaaaaatag aactcttgaa aatattaatc
    73381 ttgatattta taaaggagaa ggttcttttc taaacaacat ggtatctgga aataatctag
    73441 aactttcaaa gatatatcta gaactttcaa agatacataa aatggctttt atacaagaca
    73501 catataacca gtttctcgat aaaagagtca atgaatttgg tgtatataga aagttaggta
    73561 cagagtcaaa tggagaagtt gaatttattg gagagaaagg aactgtaata aataatggca
    73621 caataatatc atatagagat ttactatttg tagtaataaa agatgtaact attggtagtg
    73681 aagaaggtga caatagccca gttcaagctc tggaagttgg taagaaatat aatttaccta
    73741 caaattgtga atttaaacta gttgataata tatctggagt aacaaagatt actaacacaa
    73801 gaagttttga aggtggtaca gatatagaga cagatgaaga actaaaagaa agattttata
    73861 aaatccaaag aaatcaagct acaagtggaa ataaagctca ctatgaagaa tgggctttgg
    73921 aagtagatgg agtctataat gttaaggttt atccaagatg ggatggtccg ggaacagtta
    73981 aggtcttgat atttgggaaa aataatcaag ctgttgatac agaaacaatt gaaaggtgtc
    74041 agcaacatat agatgaagag aagcctattg gaccaactat aacagttgtg acaccattac
    74101 caatagaaat aaatataagt gcagtaatga aattagaaga tggatataca ttagacaatg
    74161 taaaagaatc tttcctagaa agtataaata catactttag agatattaga ggagagataa
    74221 tctatacaaa agtaatggga atacttataa atactactgg tgtacacgat ttaagtaacc
    74281 tacttataaa tggaagtaca gataatataa ctattaatga agataaaata cctagtgtaa
    74341 caactgttaa ttttagtgag gtggaaaatc aatgaagcta attgataaac taccatcatt
    74401 tgatagaaat tacattgtag aggagataca aggtgcatac gatacagaat taaatattct
    74461 taaagaagat attgatgata cctttaacca attatttgtt gacactgcaa catggggatt
    74521 agatatgtgg gaagacatac tctgcattga aaaaaaagaa cttgattttg acacaagacg
    74581 tagcaatata aaagctaaaa tgagaagcag aggtactagt actattgaag ttataaaaag
    74641 tatatgtgag gcatatacaa aatcagaaac agatataaaa gtttatagtg atgaatttac
    74701 attcgtattg agttttatag caaataattg tgactataaa actcttttag attgtagcga
    74761 tatgattgaa agagtaaaac ctgctcactt attacactat ttagaaccaa taatactaga
    74821 taaaagtatg gtctattgtg gtggaggtat ggtatgtagt gaagaagtaa aagttcatcc
    74881 atactttgaa ccaattataa aatgtagcgc tgttgtaaac tgtggagctg gaatgttaag
    74941 tagagaagaa ataaaggttt atcctttaag cattaaatgc attgaaaata attgtaagat
    75001 taatatagct attgcaaatg atacaggcgt agaaaatgta gtagtttatc ctaaatcgga
    75061 ggtggtataa ttggaagaaa aattttatat aatattaacc aaaattggta gagaaaaaat
    75121 agcaaatgca actgcactag gagagcttgt tggattaacc aagtttcaag ttggagatag
    75181 taatggagaa tattatgagc caacagagga acaaactgct ttaaagaatg tagtttggga
    75241 aggaaatata aattctctaa gaattgatga aaaaaatcct aattggatag ttatagagac
    75301 tattttacca ggaacagttg gtggatttat gataagagaa gctgctgttc tggataatga
    75361 gaataatata atagctatag gtaagtatcc agagacgtat aagccacgtg ctgaagatgg
    75421 cagtattaaa gatttggttg taaaaatgat tttacaattg tccaatactt caaatgttac
    75481 attagaagta gacccgacgt tggtttttgt aactcaaaag gatattcaag atttagatga
    75541 taaggttgat aaaaatataa aagaaataaa agtaaaaatt ggagaagaac tcttatctac
    75601 agaagctaaa aacttatcag gagctataaa tgaggtagta gaaaaaatta aaaatatatc
    75661 tattgatgat gtaataggag gtcaaataca aactgaacta tctgtattaa aaaatagtta
    75721 caataaatta tctgaaaaag tattagatat tttaatatac ttagaattag agtcagaaat
    75781 agatgtagat gaagctggat attggtatga taccttaact aatgctaaaa acataatagc
    75841 tatagaaggc cttaagttag atttaaatag aaagtgtata actggagaac ttggtagtgt
    75901 tacatttaag aatgtggttc taccatttaa tgcaaataga gttagatata tacatgaaat
    75961 ggataataac tttgttgaaa caaaatctaa tagggcatat tcaattggcc agacagatat
    76021 aactttaaat aaatattcgt atgaaataag ataattagga ggtttttata atgaaaagaa
    76081 ctaaactact tcaaagaggt aatttctttg gcgataaaaa tatggtagtt gatgaatttg
    76141 atgaagggta tgataattat gactttatta atttttttac tggatgttgt aactatacat
    76201 ttggtctaaa aaataataat atcttgtatg gatgtggaga taatagtaac tttcaacttg
    76261 gattgggaga agacaataca acaagaaaat tatttacgaa aataccaaat atatctacta
    76321 atattaaaaa agttgcatgt ggagaatctc atgcagttat acttacttca gatggagaat
    76381 tacttgtcgc aggtataaat acagatggtc aaatgggatt gggattagaa aaagtaggaa
    76441 aaacagtttc cacatttgag aaggttccag aaataaaagg cgtaaaggat attgcatgtg
    76501 gacttcaatc aacatatctt ttatacaatg atggaacttt atatgttgct ggaaataatt
    76561 tgtatggtca attaggtcta ggaactaatg gagcatctgc aaatgtaaat acatttataa
    76621 aagtagatgt tgataatgta aaggctgtat tctcatataa taaatcagct tttataataa
    76681 agaatgacaa taaatgttat tctactggtt ttaataatca aggtcaacta ggtttaggag
    76741 ataagaataa tagagattta tttagcttag tttctattaa tgatgttaag actatagctt
    76801 gtggttctga acacactgtg ttaatgacgt ataataatga tatatatggt tgtggaaagg
    76861 aaaaatgttt tggaaatgca cttcaatcat cactatttac taagatagaa gaagtaaata
    76921 taaaaactat tgcatgtggt aatggtaata ctatgcttat agataataaa ggtattttga
    76981 aggttgctgg aaataatgat atatatcagt taggtatagc aaattactct gagaatatag
    77041 ataattcatt tatagattta aaaaatattg tagctaagaa tattttcatt ggtttatcac
    77101 atagcatact aattgattca aataatgatt catattgtac aggagataat acttatggac
    77161 aattaggttc gttttttgat gatatgcaca ttgtagaatt taagaaaatg gatagtgaaa
    77221 aatatagtta tagtaattat ataaatttaa ttaaatctga ggataaatta actttattaa
    77281 aagaagaaat ggaaataaag gatattgaac ttccactaga tatacattct gtaagagatg
    77341 tcgtttttag tccttattgt actctggtta ttttagggaa tggagatgta tatggtctag
    77401 gaaataatag atacaaagga atgggttctg acttaccaag tcaattaaat gagttgacaa
    77461 aattaagtat ctctaatgta aagtctatag tagcatcaaa aaatatttct ggaggaatat
    77521 tctacattaa aaatgatgac acttgttatt attctgggcc aaatagtaac tcaatagcag
    77581 gtgttcttcc ttctaattca gatatattta agaaaatatc tatagataat gtaaaaaaag
    77641 ttgttataaa tactgattta tcaaactggt tttcattaat tgtaactaat aataagcaaa
    77701 tatatacttc tggaaagagt tcaagttatg ttaatggact tagtaataaa tcagcaagtc
    77761 aatatactga gattggtgtt agcaatgtaa cagatgcata tagttcatat aatgcaacat
    77821 ttattgtggc tgacgaaaaa aaggtatatg caactggtat aaatacaaac tatttattgg
    77881 gtgttaatcc taatgatgga gtcaatataa atttaacatt aataaataac tggttttatg
    77941 taaatccttc aggagcaagt tatagtacaa attcttgtac aaatgatatt actaaaatta
    78001 ataatattat tatatatgaa tatatggcta tattatgtac aaattatggt tcttttttaa
    78061 ctggatacca tggtacttta tggacaaagc caagtgattc tacttataga gttcaacaac
    78121 aaggaattac acatgctgga tatattagta attacatata taactattat cccacaaggt
    78181 gtacacaatc atcatattct acaaattttg cctatttgta ttatggagaa tcaacaagta
    78241 atttaaaaaa tgtaaatcca gataatttac ttatttctgg aggttcatct tatatacatc
    78301 aatatggaag gaattatctt aacaatcaat catctaataa tattgcagca tctaatataa
    78361 attcaggtcc tattacctct gataaagcca tatttttata taaagctcta ttgtatttat
    78421 cttctaacac gttatatggt tttgggaata tatctgaaag tgcaaaagaa ctagatgttt
    78481 cagatacaca agatggatat aatgccacta attataaaaa ggtaatgaaa aatataaaaa
    78541 atatatttat acctccttat gatttaagta gagataaaac tagatttgca atattaactg
    78601 ataagagctt atttatatgt ggatataact ctaagggtac gcatggtata tcagttaata
    78661 gtagtttaaa tttaaataat aagataaatt acaataaaaa gaatagcagt agtgaaatat
    78721 cttctaatat acaagaaata tatagccatt cgaagtctac atatttatta actaataata
    78781 atatgcttta cagtgttggt ttaaatgatg taggtcaatt aggagtggga gatgagataa
    78841 atagaaaggt atttactaaa ataaatattg ataatataaa atctataaat gtaaatagat
    78901 ttactgacaa tagtaaacat gtatttgcga taaaaaatga taatacctgt tatgctgttg
    78961 gtttaaataa ttctggtcag ttaggaatag gagataatgt aaatagaaat gtatttacta
    79021 aaataaatgt tgaaaatgta aaatatgtag ctgtatatgg aaacacatct ctattattaa
    79081 ctaatgatgg tcttttatat ggagcaggta ataatggaaa aggacagtta ggattgggtg
    79141 atactacaag taggaatata tttacacgta tacctataaa tggagttaga gatgtgtata
    79201 tatgtaatga tgtatcaata attgttaaaa atgataatac atgttatgta tgtggatttg
    79261 taaatggata ttttgggttt actgaaggaa gtataagtac atttacaaaa ataaatattg
    79321 agaatgtaaa atctgttgta acagcaggaa gtgaagctac attttttata acaaatgaca
    79381 atgttattta tactacaggg aaaaaagaga gggtattctt ttcaacagaa actaatgata
    79441 taaagggtat acgagtaatt aataatatta taaatgcaaa aaaaatagta gttaatggat
    79501 atacttcagc cattttaaca aatgacaata aactatttgt tggaggtctt agtggatatg
    79561 gaagtatagc aaataataat aatacaaata gtgtggaaga tgttaaagat gtttttgtaa
    79621 cagctaataa tacactttat atagataata ataacaattt gatatcatca ggtagagata
    79681 cttatggtat atctgatgaa tcttataggg atatgtcagt tccatattat aaagtatcta
    79741 taaagaaaga tgttgatact gtattttcta gttacaatac tatatttatt aaagatatat
    79801 atggaaaatt ttattcttca acaggagata atagatataa tcatttaggt attcaccata
    79861 gatatgataa tgataaaaat gaagctcttg aaggttccct acattcatat tttaaaacag
    79921 ataacacatc agataaaata gtttttaata agaaaaatga aaagctagta atgtttaatg
    79981 ataagtatat aaaaacaaat aataagtata taaattataa aaacatattt aaagataatt
    80041 ttaagtatac ttcaataata ttgccatttg aggtatctga tattgatata tcaaaaacac
    80101 attcattggc tgttgctaag gatggcaagt tatatggaat aggaagtaat tcatataaag
    80161 aaattaatca aacccttgaa gatatagaat tattaactct tactgaagta aatatatcag
    80221 atgtcaaaaa agttgcttgt ggagataact actcctatat tattaagaca gataatactc
    80281 tatggtcata tggaaagaat actgagtacc aattgggagt tggccacaat aatgatgtaa
    80341 gagagttaca aaaggttact ggattacctt ctgttaaaga tataagtata tataactcaa
    80401 tgacacttgt tttaactaat gagggagagt tgtacgctca agggtacaat acaaatggat
    80461 tatttggact aggagaaagt gaaaaagata agataataag aacttttact aaagtattaa
    80521 ctaatgttaa agaaattaag tcacataatg atgaccacat actagtaatt aaaaatgata
    80581 atagtctatg gataactggt aaaaataaat ctatgtataa aatatctata tcaattactg
    80641 atttatatga atttactaaa ataccaattc ctgaacatct aaatgatatt ttagatatag
    80701 agctttcaga tgatacaata tacatgataa caaaagtaga tacaagtaaa gcatctatag
    80761 aaatagttga aaaatcaata tctcaagtga gagttgtagt acaagaccct aataatgtta
    80821 tagaaaaact tgaaatgttt ataaatgatg aattaatatc tactaagact aatttggaaa
    80881 taaatagcat tatatttgag ataccacaaa ataaaatagt attaggagaa aataagatac
    80941 tgattaaagc cagtagtcct acaggcgatt tatattcaag tatgtttata tttaaatcag
    81001 aaacagggct taaagtaaaa aaggattcta ttttaatgat aaacaataaa gtatattcaa
    81061 tcataaacat tactgaaaat aacactgatt taatagtaac attaaatgag ggattaaagg
    81121 atgacatgat ggaaaacaat cctatatatc aattaataaa taaaactaaa gttcaagtaa
    81181 aaataaataa atctgactta ttcaaagaca tgaaactagt tgaaatcaaa aaatcagact
    81241 caagttacca agaaatctat gaattagaag aagccaacat aaaaagtgct cagcctaaaa
    81301 tcatagtaga aaaaggagat aaatggacag ctataaaacg tccatctatg atttttagat
    81361 atgatgctga aagcaacgag ccacaagctt aaaatggagg tgtgaaaatt gtttaaattc
    81421 gataaaaata aaatagaaca aatcaaacaa ggtagaaaag tagaaatgca gtataaagac
    81481 atttcagaca taagtatagg tcaagtaaag caagatgatg atataacaaa taattttata
    81541 gcaaatgcag aaatatatga gatgttgtta aatcaaagtt ctgtcaatga agcaagtaat
    81601 ataagctctt ttagtgtaag aaaatctgga ggtgagagtg gaatggtaga agtatatgta
    81661 gctttaattt taagaggcaa aaaaacaata gaagaagtac cagcagtaat tagagagcaa
    81721 gttagaatta gatgcaaaga attagaaata ccagttgaat agtaaattta gaataactat
    81781 gtattagtta ttttttttat gtaaagtaca aggtcttaac tttaataagt aagccttgta
    81841 cttatttttt gttatattag aaattgtata tatatttatt atttattcaa tctataaatt
    81901 gcacctacaa tttaaagtac agaagattaa attgataatc ctgaaaatat aatattgcat
    81961 gatgtaagaa tataacaaaa attaaagcta taagtataaa aaatttagac aataggaggc
    82021 tataatggat aaattaataa ccgaattgag tagtctaggg gcaataggta tactatgtgc
    82081 tctattattt aaaaatacta tgcaggagaa aaaagaagat agagacatgt ataaaaaaac
    82141 tgtagaaaat tttatagaat tatctacaca acaacaagaa ataaacaaaa atatacttgt
    82201 tcaaatgggt ataatgaaaa cagatgtaga ggaaattaag gaagatgtta ctggtataaa
    82261 aggtatgtta caaaacggtg tataacatga aagagtagca ccagattata tattgttagg
    82321 aaaagataaa gtagtattgt agatagtacc ctattttatt gagaaggatt tgatatttaa
    82381 aaatattaat taaaaaaagt aataaaaata atatataaaa ataatatata aaaattaaaa
    82441 aaggagttga gcttaaattt gattagaaaa aatcaatttt aagacaactc ctttttttta
    82501 ttaaattatt gtctattaac caaaatagct attttagcat ctggattata acttatttga
    82561 accatttgat ttttcttaac atgttcaagg tcttcaccac cataagctat ttgtaactta
    82621 actggtaact taccttgttt tataatagca acgtactctt ttttaccttt tttctctaaa
    82681 ctaatcaaat tgccaacata aggtttaaag ttctgatact ttttactaga atttcttatg
    82741 tagaagaaag caccaacagc aataactaaa tttatgccaa gtgtaaccca agaattgatt
    82801 ttaagcatag ctccagcgat tattatcacg aacattaaaa cgataggtaa tatagctttc
    82861 ttaagaagca atttacccat tatttcatta gcctttttct cagggccact catagttttt
    82921 gatctagcaa atgattgcgc gaatttgtct cttaagccca ttttatcctc ctaattttaa
    82981 taaatattta gttataataa cgagatatta cttgaaacta aaaatttact acatttatat
    83041 tatgtttgac ttttgtataa ataattacat tcaagtaaag caaaatatac taattatttt
    83101 atcataaaat tataaaaaag aaaataaatg aaataaaaat attagaacaa agaaatgatg
    83161 taaaatcgta tcaaaagcaa cataaaaatt atttatctat tttctcatct ttatttttgt
    83221 tatactcaat ttttcctaaa tccttctctt tttcatattc atgaagtttt aattcaatca
    83281 taccttctat tttggcttta tcataatcat ttaactttct aaaattgttt aaaagcttta
    83341 tttcattaga gtttatgcta ttaagtggat agtttgagga ggaatcgcaa attaaatctg
    83401 atttatgtga tagattatct ccatttaata gccagtctac tgaaacatta aatatctcag
    83461 ctatagattt taatatttca taatttggtt ttctaatgtt tctctcaaat ttacttaagt
    83521 tgtcacagcc caacatttct tctagttcat attgtttaag gttttttgct tttctcaaat
    83581 aaacaattct ttctcctaaa gtatccataa acactctcca ttcaattaat gtcaaaaaga
    83641 ctttttaaga tgtaaatagt ttcaaattta aggtcaaaat gacataaaaa gcattgactt
    83701 aaggtcaaaa tgactttata attaacttaa tgatacgaat ttacatccta attttagcac
    83761 aaagtaatca aaaaatctta tttagtatta aataaattta tatacttaat atgtgtacat
    83821 attaaaaata tatactaaat agagggggtg tgtaagctaa aataatataa aagtaaatat
    83881 aaatcactta gaaaggaagt tgataaatgg atgctcgaaa aaaatggata ccttttttgg
    83941 gagtgcaagt caagcaaaga cttattgaat taaatatgac tcaaagggaa ttagcgaaga
    84001 aaataggtgt taatgaaaac tatttgtcag ctattttaaa tggaagaaga acaggtaaaa
    84061 aatataaatc atcaatttat caattactta atatagaata ttcagaagat gattaataaa
    84121 tagtatataa agtaggtgaa tattcttgtg tgtaaattgg attcagatgg ggttatagag
    84181 tgttgtagag caattgatga ttttattaca gcactcagta atataaaaag cttaaatatg
    84241 gaaagattaa atactttaac taaatattct agtacatgtt caatccttct taaagagggg
    84301 aattatgaag gatgtacaat tgtgtataga aagatgttgg aagaattaaa aacatgagta
    84361 atgcatttct taggaatata agttatacat agagatgtat tatatttttc aaagtactta
    84421 aactaaaata tggataagat aatctaaata ttataaatgt gcttgaaatt agactatact
    84481 tgtttttaaa taatccaata tccatatttt agtaatatac tacaaaaaaa gaaggttaat
    84541 agatgatgta aaatcgtatc aaattatgta tgtttaaacc attttatctt cattattatt
    84601 agaggaatgc ttttttaagt ctttatattc agatatctta agttcaagta ttccttctat
    84661 ttttatttta tcacgttcgt ttagttgtct gtatagattt aatatcgtca tttcatcatt
    84721 agtaacatgt aagtaatctt ctttatcttc ttttacacta ctattgacat ttaccttctc
    84781 tttaccatag agaagccagt cagtcgtaac attaaaataa tcagctattg acattagtat
    84841 atcacaatta ggttttctat ctcctgtttc atacttgcct aagttttcaa attttaaaat
    84901 atccataagt ttgcgctgag taagtttttt ggagtttctc aaataagcaa ttctttttcc
    84961 taaagtatcc acaaaataca ctcctttctt tttatgagta atgtctaaat gacatttaaa
    85021 attaaaaata tataaattta taatataaaa ctactaaatt aaagtctaaa tgacattttg
    85081 cttaaattaa tatgctcata atatgatttt aacatattat agttgaaaat atatggttta
    85141 ttttgatttg tatatataac aataggttta attgttatat aaatgtaaag gggtgtatga
    85201 atagattgta taaatttatt tcgataaact aagattgctt tttgattgtc tgtaaaagag
    85261 aaaaagatta agagaaaaat agtattatat tgtaatttat attaatcaat tacaaaaatt
    85321 ttatgaattt attctttaga ataaaatatt taagaataa
//
LOCUS       DAMJVK010000005        81195 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.005, whole genome shotgun sequence.
ACCESSION   DAMJVK010000005 DAMJVK010000000
VERSION     DAMJVK010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 81195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 81195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..81195
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.005"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            31..1143
                     /locus_tag="PC414_000346"
     CDS             31..1143
                     /locus_tag="PC414_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HDF2462761.1"
                     /translation="MNSRIKSIDIIRGLSIALMIVCNNPGTWMRMYPQLRHAVWHGVT
                     LADFAFPFFVISLGVTIPISINSKLKNNKSTLSIILSIFKRSILLILFGFFLNYLGNP
                     DLDTVRILGVLQRMGLVYFVTSLVYLLLKKLNVGSTATIITFLCISTFIIVGYYILAK
                     PYGFELEGSLAQLVDLHFFKGHLYKPEFEPDGFLTSIVAISSGMLGCTMGCVLLKENI
                     GEYKKFFKILVMSIILLIGAFIFNQYFPFNKRLWSSSFVLLMAGSYGILLSIFYFICD
                     IKNKSKIFTPIIALGSSPIFTYMCLEILSHVFWNVPKLTNKVDYPTTLVEWTTYELIT
                     PWAGTTWDSLIFSLLYVLFWVIVMSIMYKKKIFIKI"
     gene            1397..3421
                     /locus_tag="PC414_000347"
     CDS             1397..3421
                     /locus_tag="PC414_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2462762.1"
                     /translation="MKKTTKLLATGMLSVAMVAPNVALAAENTTANTESNSDININLQ
                     RKSVVLGSKSNASVKFKEKLNADSITLNFMCYDMPLEATLNYNEKTDSYEGVINYNKD
                     PEYLNVWELQSIKINGKDEQKVLNKEDLESMGLNLKDYDVTQEFIISDANSTKAVNEY
                     MRKTSAPVKKLAGATRFETAVEISKQGWKDGSSKVVIVNGELAADGITATPLASTYDA
                     PILLANKDDIPESTKAELKRLNPSDVIIIGDDGSVSQKAVSQIKSAVNVNVTRIGGVD
                     RHETSLLIAKEIDKYHDVNKIYIANGYAGEYDALNISSKAGEDQQPIILANKDSVPQG
                     TYNWLSSQGLEEAYYIGGSQSLSSKIIDQISKIAKNGTSKNRVSGADRHETNANVIKT
                     FYPDKELSAMLVAKSDIIVDSITAGPLAAKLKAPILITPKTYVSAYHSTNLSEKTAET
                     VYQIGDGMKDSVINSIASSLSKHNAPTEPDNSGSAAGKTVVIDPGHGGSDSGATSGLN
                     GGAQEKKYTLNTALATTEYLRSKGINVVMTRDTDKTMALGERTALSNTIKPDLFTSIH
                     YNASNGSGNGVEIYYKVKDKNGGTTKTAASNILKRILEKFNMKNRGIKTRTLDNGKDY
                     LYVLRNNNYPAILVECAFIDNKSDMDKLNTAEKVKTMGTQIGIGIEDTVK"
     gene            3641..4327
                     /locus_tag="PC414_000348"
     CDS             3641..4327
                     /locus_tag="PC414_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDF2462763.1"
                     /translation="MSGYTNDECEIPKIKSYPGADKEIASEIDYSIVKGTVLFDLYQR
                     IMDLVLSIIGLVIGLPLIAIFGILIKIEDKGPITYKQERLGKCGRRFYIYKLRSMRTD
                     AEKFGAQWAEKDDPRITKVGKFIRKTRIDEIPQLFNILKGDMGLIGPRPERPNFTVQF
                     NEEIPGFINRLAIKPGLTGWAQVNGGYEITPEEKLKEDIYYIKNRSILLDFKILFKTV
                     KVVLTGDGAR"
     gene            4422..5477
                     /locus_tag="PC414_000349"
     CDS             4422..5477
                     /locus_tag="PC414_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp7"
                     /protein_id="HDF2462764.1"
                     /translation="MIKKISTILSLVLLISISSTIGVFADANPKRELIEGSIPEISTE
                     LNKRAFKDSKEVILVNEESIVDSISATPLAYAKNAPIVVTKSKNLGRVTRNYLKELGP
                     EKVTIVGGLKAVSKDAERNIEKMGMKVERIRGKDRYDTSLKIAREMYRTVGFDEAFLL
                     SSTTGLENAISVYSYAAKSGMPIIWAKDEGFEEQIDFLKGKNLKKIYALGDSKEFIAE
                     IDSNLKNIEGIKQINKSSTNVDLIKKFYDEKDIKKIYTARLDFGSRSDVNEYISLGVV
                     SAKENMPILICSDNLSRAQDKFLKDSNINDVVEVGYTVGDYSLFKSIFNLTFLSCIVL
                     ILLLLLITFRALRYESK"
     gene            5592..7148
                     /gene="murJ"
                     /locus_tag="PC414_000350"
     CDS             5592..7148
                     /gene="murJ"
                     /locus_tag="PC414_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HDF2462765.1"
                     /translation="MSKTAKAALWIMAATMFSKVLGFLRELVLANFYGTGMYADVFVL
                     TLNIPGLIIAVIGSAVATTYIPMYFETKKRLGDEGALKFTNNVLNICYIMAIVIAIIG
                     LLFTEQFVTVFAAGFRNDPAKFQAAILFTKIMISGVLFLSGSKIFSSFLQVNDSFVIP
                     GLIGIPYNIIIIAAIALSAGKNVWILPAGALLAMASQLLFQLPFAFKKSYKYKPYINL
                     KDESIKELVNLVLPMLVGVAVGQLNIFVDRLLATTLGDGKLSALNYANRLNEFVMALF
                     VTSIITVIYPKLAKMSGKDNKEGFISTIVKSSNCIILVVLPISIGAIILAEPLVRILF
                     QRGKFDALSTDLTSIALRLYSLGLLACGVRDVLYRAFYSLSDTKTPMINGSIALIINI
                     VLNLILIRPLGHAGIAISTSTSNIITVILLFISLKKKNGYFGGDKIIKTGLKSLVASG
                     VMAVATLLIYNNLYAFMGSGTIKEIISVGAGVLGGASVYTVLIVLFKVEEMDLAFEFL
                     RKGKQKLLRR"
     gene            7194..8900
                     /locus_tag="PC414_000351"
     CDS             7194..8900
                     /locus_tag="PC414_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HDF2462766.1"
                     /translation="MDYKNNYEMWLNSPYFDEQTKNELLSIKDDEKEIQDRFYKNLEF
                     GTGGLRGIIGAGTNRINIYTVRRATLGVLNYIMKTQGEEGKQKGIVIAHDSRYMSREF
                     CIEVAKTLSAYGVKAYIFEELKPTPELSFAVRYLKCAMGIVITASHNPKEYNGYKVYD
                     SDGGQICIDMANDIIAEVNKIDDYSTIKSIDFEEALSKNLITILDNEVDDEFIKAVKK
                     QVLRQNIIDEYGKKLKIIYTPIHGTGNKPVRKVLNECGFENVMVVKEQELPDSNFSTV
                     KYPNPEEKSVFNIAIEMAKNNGTDLIIGTDPDCDRVGIVVKDSSGEYVVLNGNQVGSL
                     LVRYILESLVEENKLPKNNPTIIKTIVTSELGAKIAKAYNVDCLNTLTGFKFIGEKIK
                     AFEESNDRSFIMGYEESYGYLIGTHARDKDGVVSSLMICEMAAYYSSKGMNLYEALID
                     TYNKFGYYKEDLKSVTLKGIDGIKKIKEMMLYFRSVKIDNVADVKVDKILDYKDGVDD
                     LPKSDVLKFLLEDGSWIAIRPSGTEPKIKFYFGANSDNQEDVEFKLNNLISYILNVVD
                     SI"
     gene            8922..9995
                     /locus_tag="PC414_000352"
     CDS             8922..9995
                     /locus_tag="PC414_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HDF2462767.1"
                     /translation="MKVYNVIMAGGGGTRFWPLSRQEVPKQLINLSGEDALINETINR
                     IDSLAKKDDLFIVTNEKQLEALKDIVKDKCLDSNILPEPCARNTAAAIGFAAFNIMKK
                     YGDGVMCVYPADHYIKDEKEFKSILEKAIYIAENNDKLVTIGITPTFPSTGYGYINFN
                     RENTIEDVAYEVVEFVEKPNYEIAKEYVNSKKYVWNSGMFVWKVSKILEDFKRYLPKV
                     YEKLEDISKYLGTKEEMEKIKEIYPTIQSISIDYGIMERSNDVIVVPGDFGWNDVGSW
                     DSLGAIYPTDDEGNIKRGENITIDTKNSIIYSDDKLISTIGISDLIVVSTNDAVMVCR
                     KDKAQDVKKIVEQLKEEDRQEYM"
     gene            10007..10729
                     /locus_tag="PC414_000353"
     CDS             10007..10729
                     /locus_tag="PC414_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDF2462768.1"
                     /translation="MIPKKIHYVWFGGPKGNIENICINSWKEKLPEYEIVEWNEKNFD
                     IEKEIKGNKFLEECYKRKLWAFISDYTRIKVLYEQGGVYMDTDMQILKDITPLLENNR
                     LICGYEDDREYINGAIIGVEKGHPFLKDLLEYYEKEVLTSSLFTIPKIMTHLMEKNYK
                     KIDPNNYEEGIRVYDKEYFYPFGFKEDFTPECITENTFGIHWWGKSWAKKRNYFLESK
                     HLTGVNKIWKCCKIFASNTLRS"
     gene            10745..11902
                     /locus_tag="PC414_000354"
     CDS             10745..11902
                     /locus_tag="PC414_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide polymerase"
                     /protein_id="HDF2462769.1"
                     /translation="MKTLNDDRNIMIRKYLYIFVLFIILLRKYMISFIDPNMDIGMIK
                     SALFYSSIGILMLLFLFDKRKSITEMVLVGVCVLLYLLNREGAILLIVLLAVSAKQID
                     DKFIVKNYLIISACFLMVAILLFNLFPSLIFNQEVHYRYIEKIDMLVTRMDFGLGNPN
                     SVFYHMVTIYAAYIFLRYKDYNKWDRIILFGSAFFVYQTTYSRTGFFTILAGLIFVEI
                     IRWIDIKKIKGLPMLLKTLPIILTLFSVIIGTVFDKSTLLNRLLASRPKFWHVYLAEK
                     GNFLKPFGNSYSPAIKATNPLDSSYVYIISVLGVVACILFMYLMYKGIESFIEKDKKA
                     YLVALFIFLLYSFAENLLLEASFSFAVVLLIKEVILNDKREIDLWKMKSRR"
     gene            11905..12744
                     /locus_tag="PC414_000355"
     CDS             11905..12744
                     /locus_tag="PC414_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2462770.1"
                     /translation="MLISLIMPTLNRYDDIYLLMDSLENQTYKNFELIVVDQNDNSKV
                     KEIVDKYIDKLDIKYIKSSKKGLSYNRNVGIDNAVGQIIGFPDDDCVYENDTLEKVIN
                     FFNKNKDYKIYSCKTMDSNKVDAFKKMYDGTCDITSSNVLDTITSITFFIDFEGKDYT
                     RFDEKLGVGGEFGAGEEIDYVLNLLSLGFKGKYFGNDIIYHPAKKHSKSKEKYQKDYN
                     YGRGFGALCKKEIVYRKNYKFAKVMVSKLVRNIGGLILSSNRDYHSATIKGRINGFRQ
                     YKL"
     gene            12759..13934
                     /locus_tag="PC414_000356"
     CDS             12759..13934
                     /locus_tag="PC414_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HDF2462771.1"
                     /translation="MDKVKFLINMLLAFFIYPFNKHKFNGRDIWLIGGHSGDIYNDNS
                     KFFYEYMLKEHNDVETYWVVNKDSKVFDKIPGKKLIRGSVENYLYYYNSKAIVFSHAP
                     SADIAPYNFAVPVLNYFHKKTIKVFLNHGTISFKKRKPMNKKFKNIIDNLYKSYNIVT
                     ASSEFERNVMVNDWGMLDDSVYIIGNARYDNLPTNEVAQTRDILYTPTWRDWIKFSSG
                     KFTDTDYFKNIMNFLNDDKLNKILDEKDINVKIYMHHLMHEFIDDIKENITGKRIVFL
                     DKGVTLANEIRKSAANITDYSSVAIDFLYMNRPILFYQFDLDEYMEKVDSYIDLKSEM
                     FGSLAYNNDEAVNKLIDIIENNFEVMDNQKNERNKFFRYNDNKNCKRIYDCVLSKIK"
     gene            13963..14712
                     /locus_tag="PC414_000357"
     CDS             13963..14712
                     /locus_tag="PC414_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2462772.1"
                     /translation="MKKNLVSIITPMYNSEKFIEATIKSVLNQTYQEWEMLIIDDCST
                     DNSPNIVKSYMQQDSRIKCIKTETNKGVSNARNLALSKATGQFIAFLDSDDQWNSSKL
                     EKQVNFMLENDYVISFTSYELMDENDKKLNKVIKVPPNVDYRRLLKGNILGCLTVVID
                     KSKLDFEIRMSGVRHEDYVLWLSILKKGHIAHGINEVLALYRKSSNSLSGNKIKAAMW
                     TWNIYRNIEKIPLYKAIYYFINYGINGIKKS"
     gene            14734..15642
                     /locus_tag="PC414_000358"
     CDS             14734..15642
                     /locus_tag="PC414_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2462773.1"
                     /translation="MYKYKFTVFTPTYNRAHLLENLYNDLKAQTYDFNDFEWLIVDDG
                     SSDSTKELVEKFISENKLNIRYIYKENGGKHTAINLGVKNADGELFFIVDSDDGLIKD
                     SLEIANDEWDSLENKEGFSGVVGLCVYPSGDLIGTEMPEDKKICHFADLYFKYGVKGD
                     KSIVFVTDELIKFPFPERKEVRFLPESVVWNEMSKYYKVICVNKPMIIRDYLDDGLTK
                     NILSKNALRGRALEFLYLINQNTYPLSRYPYMWIKNYINLARYSLLSDSHYFGELRKV
                     SDKLLYLALFPLGYYKYIGQRKLVSK"
     gene            15690..16442
                     /locus_tag="PC414_000359"
     CDS             15690..16442
                     /locus_tag="PC414_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2462774.1"
                     /translation="MNEPLVSIITPVYNSEEFLSETIKSIQNQTYKNWQLLLVDDCSK
                     DNSSEIIKSFRKEDARIKYIKLEKNSGAAVSRNVGIKNAEGRFIAFVDSDDLWDSRKL
                     EIQIEYMLKENVGFSFTSYRYMRQDGSKTNKVARAPKKIDYEGLLRNTIIGCSTVVID
                     KEIVGEFSMPLVRRGQDTATWLQLLKKEKYAYGIQEDLVNYRLVGNSISSNKIKALKR
                     TWNTYRNVENLSLPKSLYVFCFYVFNAIKKRV"
     gene            16396..17763
                     /locus_tag="PC414_000360"
     CDS             16396..17763
                     /locus_tag="PC414_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HDF2462775.1"
                     /translation="MYFVSMFLMPLKKEYKFYILGGKKMKIAIAGTGYVGLVTGVCLA
                     EVGNENVVCVDVDEQKVKSMKLGIAPIYEEGLEELMVKNYNRGRIDYTTDYKKAYEDV
                     DVILIAVGTPERSDGSANLDYIKAVAKQIAETVTKDTLVVIKSTVPIGTNEEIEDYIN
                     RNLKNDVKVELASNPEFLSQGTAVKDTLHASRIVIGVESEWAREILEEIYKPFNQPII
                     VTNRNSAEMIKYASNNFLALKISFMNDIANLCEIAGANIEDVALGMSYDDRIGSKFLK
                     AGIGYGGSCFPKDTKALHWLSEHNGYELKTVKAAIEVNESQKLKLVRKASKLVESFQD
                     MKVAVLGLTFKPDTDDIREAPSIPNIKYLLERGARVTVYDPIGVNNTKKIFGDSILYA
                     DSVDDAIEDAEICFIMTEWKDVLNYDIHKYKEKMKNPLVLDGRNCYKIKDMEELGIEY
                     YSIGR"
     gene            17769..18860
                     /locus_tag="PC414_000361"
     CDS             17769..18860
                     /locus_tag="PC414_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDF2462776.1"
                     /translation="MKTVCYLADGSNPHTLKWCSFFKNKGYDIHVISLNGGHMEGIKI
                     YNFGSNVEELKNENISKKTGYLGSIKQIKKLVNEIKPDILHAQYASSYGFIGSLLGYH
                     PYIISVWGTDIYDFPNNGFIQKQIIKHNFRKADYIFSNSRDMAKEANKYTAKHVDVTF
                     FGVDMDRFKPMEVEKEDAFVIGIIKSLEKKYGIEYLIQAFKMLKDEYKDKKIILKIGG
                     SGSQMDNLINLAKELGIENDVQFLGRISPENVSKTFNSFDVTVFPSLREGFGVAAIES
                     EACEVPVIVTNVGGHPESVWENETGLIVEPKQPEEIKNAIIKLMENDELRLNMGKKGR
                     QFVRENYEVNLNFNDIEKIYDSIFDKYKK"
     gene            18944..20857
                     /locus_tag="PC414_000362"
     CDS             18944..20857
                     /locus_tag="PC414_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDF2462777.1"
                     /translation="MRESIDLEKESFFNRLFKKYKFRHLTLLILDICSVLVAFRMSLS
                     LTGNLNAFTTNDIIISACIYIVLHIVSFRLFKCYNTLWRYAGEEEIISIFVATLAYLI
                     PIYIINKLLGFDYPIMFYVLNTIFIIMFTSGARIAYRAIRIVMNKTYSRGKVSNILII
                     GAGDAGEMVIQELKRNPELKKVAVAIIDDDKNKIGRIIHNVKIVGTTSDIKDVVEKYN
                     VDEIIFSIANIEKRRKKEIIDICKNTNCKIKTIPGIYEIIDGKVDIKQIREVEIEDLL
                     GREPIKTNLREISNYIEGKVILITGGGGSIGSELCRQIAGFNPKELIIVDNYENNAYS
                     IQQELLRKYKNKLDLKTVIASIREEKRMDEIFNKYKPEVVFHAAAHKHVPLMESSPGE
                     AIKNNIFGTLNIAGLSSKYRAKKFVLISTDKAVNPTNIMGATKRAAEMIIQTMNAESQ
                     TEFVAVRFGNVLGSNGSVIPLFKKQIEDGGPVTVTHPDIIRYFMTIPEAVGLVIQAGA
                     MAKGGEIFVLDMGEPVRILDLAKNLIKFSGFEPDVDIKIEFSGLRPGEKLYEELLMSE
                     EGLLDTEHKKIFIGRPIDVDREKITKYLKLLREITNNEEVEKIDGIMRELVPTYIKPE
                     DANIKEIATTREK"
     gene            21056..21763
                     /locus_tag="PC414_000363"
     CDS             21056..21763
                     /locus_tag="PC414_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDF2462778.1"
                     /translation="MMNIKNKKHILKKFIAMVLIAGVVTVEAGAITASAAEPTNSPMS
                     ATVDQCDFLNVRSGASANDAVVGKINTGDKVEVLELHSNGWIKIKSVDNVTGWVNGDY
                     LTIQGGNVDAKVQNVLNLAFKQQGKPYKWGATGPNSFDCSGFTSYVYKNGAGVNLPRV
                     SRSQATVGKKVSRAELKPGDLVFFGSGGSINHVGLYVGDSKFIHSPQTGDVVKVTSMA
                     PGTNYARRLITATRVLQ"
     gene            22065..24176
                     /locus_tag="PC414_000364"
     CDS             22065..24176
                     /locus_tag="PC414_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding glycosyl-hydrolase Cwp19"
                     /protein_id="HDF2462779.1"
                     /translation="MKKISILVLSLIMTLTMCSVSSFADSSNDKEMRAAWISTVYNLD
                     WPKTKNNEAKQKKEYTDLLDKLKSVGINTAVVQVRPKSDALYKSNINPWSEYLTGTQG
                     KDPGYDPLPFLIEEAHKRGMEFHAWFNPYRITMADESIDKLPANHPAKKNPSWVVKHG
                     NKYYYDPGLPEVRKYIVDSIAEVVQNYDIDGVHFDDYFYPGVSFNDTATYQKYGKGQN
                     KDDWRRENVNTLLRDVKASIKSIKPNVVFGVSPAGIWRNKSSDPTGSDTSGNESYVGT
                     YADTRAWIKQGLIDYVVPQLYWPIGLKAADYSKLVAWWANEVKGTNVDLYIGQGIYKQ
                     GQSSYGGQNIAKEIVQQVTLNRKYSEIKGSMYFSAKDIANSTSIQKDLKSLYSSSEEP
                     VTPPSNVKVEKLRGDERYDTAVAISKKGWATNSDTVVLVNGYSIVDGITSTPLATSND
                     APILLVNKDNIPTSTKNELKRLNPSKVILIGGNNSIGDKVESEIKDTLSNVSINRVGG
                     SDRYSTSLMIAKELVKTNPVEKLYITSGTGEADSLSIASKAGEEKQPIVLVSKDNVSD
                     EVYNWISDLKVKDAYFIGGNLSISDSVINKLDKVITNDVSKNRIAGENREETNGKVIQ
                     KFYPNAEYSSMFVSKSNQLVDALTSGPLAAKLKSPVVMLGNSVTSAQKTALEPKKTTL
                     VYEAGDGINQNTLNTFLNLVK"
     gene            complement(24374..25339)
                     /locus_tag="PC414_000365"
     CDS             complement(24374..25339)
                     /locus_tag="PC414_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDF2462780.1"
                     /translation="MSKLKKFVILLAFLVVIFPISVYGYFYYKLSAIHDSSISSDLLD
                     NNDHKNEDGIINILLMGTDARPNEDSSRSDAMMILTIDNKHNDIKLTSLARDSYVDIP
                     GHGKQKLTHAYAYGQADLLIQTIEENFNIDIQNYACVNFESFMYIIDAIGGVEVTVEK
                     GEIRELNKFIPETYKWNKSDDKGSIQYIRNSGKQTLNGYQALSFARIRHNDTAFARDG
                     RQRQIIQAIIKKTETLPVTKYPGLLDAVLPYVKTNMKPNAILSLGAQVLKMGDLNIKQ
                     FEFPIDDEIHSTGGIYGKAGWVLRFDPDTLDILHDFIFNDIEFKQ"
     gene            complement(25624..26580)
                     /locus_tag="PC414_000366"
     CDS             complement(25624..26580)
                     /locus_tag="PC414_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDF2462781.1"
                     /translation="MSGLKKFVIFLALLVIAFPVSIYGYVFTKLKTIQDDSINTEALS
                     SNDYKNEENITNILLLGTDARPGEEVSRSDAMMILTIDNKNKSLKLTSLNRDTYVNIP
                     GRGEQKLTHAYVYGQANLLIETIEENFQLDIQNYAVVDFFSFMDIVDALGGVTVTVDK
                     SEIRELNKFIPETYKWNKNDNKGSLKYIRNSGEQKLNGYQTLAFSRIRKNDSTDERDR
                     RQRSVIQSLINGVKDLPVTKYPNLVNTILPYVKTNMNPNEIISLGKELLSIGNLNLKS
                     MEFPLSTENGRKIGNAGYVIPFEEYELDAMHDFIFKDIMVED"
     gene            26948..27736
                     /locus_tag="PC414_000367"
     CDS             26948..27736
                     /locus_tag="PC414_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF2462782.1"
                     /translation="MIKLIATDLDGTLLDEKSEINPEFYKVFKKLRERGIMFSAASGR
                     QYQNLIKKFEDIKDDMMFISENGTLVVYKGKEILSNPLNKELVNEIIETTRSIKGKKI
                     VMSGKKYAYIESKDEAFIQEVSTYYAKFKIVEDLTKVEGDILKIAVFDFKGAEHNNNI
                     YFEKFSDRAQVCISGVEWLDLTAKGANKGSAIKKVQKMLDIKYEETMVFGDQLNDVEM
                     MKSAYHSYAMENANEHLKQIARFRAKRNTENGVVDKIKEVIKIG"
     gene            complement(27859..28329)
                     /locus_tag="PC414_000368"
     CDS             complement(27859..28329)
                     /locus_tag="PC414_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4358 domain-containing protein"
                     /protein_id="HDF2462783.1"
                     /translation="MKKILILFFSIILSLGMVSCSSSGDYKNVSTSKIKKALKSSDLL
                     IKESKIFDAKDFNYFNDVEEDIIQGFVINSSEKLSLEDIIVIKTDEVDKIYKTLENYK
                     KDMIQAPFGEGHGEKINSEIANNTIIEKAGRYVYLISAENATQIENKILNIIKK"
     gene            28468..29112
                     /gene="uppS"
                     /locus_tag="PC414_000369"
     CDS             28468..29112
                     /gene="uppS"
                     /locus_tag="PC414_000369"
                     /EC_number="2.5.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-trans,poly-cis-decaprenylcistransferase"
                     /protein_id="HDF2462784.1"
                     /translation="MRIPKHVGIIPDGNRRWASQNGMTKDKGYESGIDPGLESYRLCR
                     DLGCEEVTFYGFTTDNTRRPSVQTKAFTKACIDAANILIDEGAALLVVGNTKSPMFPE
                     ELLPYTNRIKAKEGQIKANLLVNYGWYWDLNNISGVENVNKNNIQDYIQSFDVSRLDL
                     IIRWGGRRRLSGFLPVQSIYADFYVIDNYWPDFKSDDIKDAFSWYDTQDVTLGG"
     gene            29155..30060
                     /locus_tag="PC414_000370"
     CDS             29155..30060
                     /locus_tag="PC414_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2462785.1"
                     /translation="MKKKLLDGKITLIICKSVKIYTKKGEEMSKGNNNNNSRNKSKKT
                     SHLNRKKRKLNKKKLAVLICFTVLFLFIAFKATQGVVALVKSMDKSNKTNQQQNVNSE
                     QFDLGNEEENKKKKYTVFIDPGHGGNDKGTESKTSNRYEKDLNLQIAKKLANKLSKQK
                     DIQVVVSRTDDTYISLKDRAILANNSSADVLVSIHLNAEKNGNTATGIETWYRNKATD
                     GSKELAQTVQSTIVSYVKVRDRGIVENNFEVLRESNMPAILIECGFLTTPSEEQKIIN
                     EKYQDQLAEGIVQGVLSYLDSKGNK"
     gene            30375..31922
                     /locus_tag="PC414_000371"
     CDS             30375..31922
                     /locus_tag="PC414_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2462786.1"
                     /translation="MNKNSMTRIYSLNKIIFIIIGILGIVLLTRHIEVDIKVLILILL
                     TLFSINVFISYLKIKLYEDKINLGLINNSDKFINITKDENFLKHVQNYIISNEKENCV
                     MACFDLCRLKLINDIYGYELGDEVLNNILSNLKNYFGKEAIYGKLKSDVFVLIIKLEN
                     REQKIPYLVNLIKNKIVILSQIDSFKMDINIEASIGIYKFNNNEIDIKKAIDNADMAR
                     LKSKGLKHIEYVEFDNAMEEEIQSIMKIERDLFLAIKNKEFVIHYQPKVDSSTGNIIG
                     SEALIRWLHPSLGMVGPNKFIPIAEKNGLINNIGRWLIQEVFITINKWISEGINVLPV
                     SINLSRVELYKNDLVDFFKLMFNTYNIPKELIELEITETTALRDVKFISERLYEIKSL
                     GMNISLDDFGVGNSNFINLKGIPLDIIKIDRSLILDIVTNTKTKFMVKAIVNLSHDLN
                     VTVICEGVEDMHQVKVLSELGCNFIQGYVFFKPLDEMNYKKLLTDGSYINLKDTLLDK
                     CMTVEEE"
     gene            31925..32323
                     /locus_tag="PC414_000372"
     CDS             31925..32323
                     /locus_tag="PC414_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDF2462787.1"
                     /translation="MISEDVDYSIRIIRAIFNNGEDKPIDAKMILKSEDIPILTLYRL
                     LRKLCANNILTFNKEDGKTYMLNVSLDTISLRTIVEVIDGKIYINKCTRYVSNCSNCL
                     KGCKVNRELLEIEKEIYKLLSRKSIRQILE"
     gene            32363..34096
                     /locus_tag="PC414_000373"
     CDS             32363..34096
                     /locus_tag="PC414_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HDF2462788.1"
                     /translation="MDDISQDNFLLSKEYENSLDVDTKKASGIYYTPKIIVDYIVKKT
                     LKNHDIIKNPYPRILDISCGCGNFLLEVYDILYDLFEENIYELKKKYDENYWTVDNIH
                     RHILNYCIYGADIDEKAISILKDSLTNKKVVNDLDESDIKINLFCCDSLKKKWRYKFD
                     YIVGNPPYIGHKKLEKKYKKFLLEKYSEVYKDKADLYFCFYKKIIDILKQGGIGSVIT
                     PRYFLESLSGKDLREYIKSNVNVQEIVDFLGANIFKNIGVSSCILTFDKKKTKETYID
                     VFKIKNEDICINKFETLEELLKSSKFEHFNINQRLLSDEWILVNKDDETFYNKIQEKC
                     KYSLEDIAISFQGIITGCDKAFILSKDDVKLNLVDDKFLKCWIKSKNINKYIVDKSEY
                     RLIYSNDIDNENTNKRILDEIIGLYKTKLENRRECKSGIRKWYELQWGREKLFFERKK
                     IMYPYKSNENRFAIDYDNNFSSADVYSFFIKEEYLDKFSYEYLVGILNSSVYDKYFKI
                     TAKKMSKNIYDYYPNKVMKIRIFRDNNYEEIENLSKQIISILLNKSIDKGKVEKLQIK
                     MDNLIMDSLGI"
     gene            34328..35539
                     /locus_tag="PC414_000374"
     CDS             34328..35539
                     /locus_tag="PC414_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDF2462789.1"
                     /translation="MKGLVLEGGGTKGAYQIGAYKALRELGIEFQGVTGTSIGALNGA
                     YIVQNDIEVMEDIWLNYDYTHFMNIDRETYERFKNVDFTTKNLNAVFELINKARKNEG
                     IDISPLRQLMETTLNEDIIRKSKKDFGLVTVWWDKKINPYPLYIEDIPKGRLIDYLIA
                     SSSLPIFQTNMMDDKLYLDGMFCDNMPIGLLEDKGYEDVVVIRLIDDFIGKMNLNRHQ
                     NINIKTIIPSEYLGGSLNKDRDNVEKNIRLGYLDTMKAYERCTGIKYYFDVEYKYNEE
                     YCFDKFRSMSNETIQYICNLLNIKRDASLRTVMESVIPKLGEVLGLPKDFSYKDLFYC
                     IYEKKLEENNINRIKLYDFNKVIDVVNTNIDSNRNLASNFEVRPKISIKKNKNTKLLT
                     NCIIKDIKNQS"
     gene            35732..35992
                     /locus_tag="PC414_000375"
     CDS             35732..35992
                     /locus_tag="PC414_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HDF2462790.1"
                     /translation="MEKVVSIKNASGLHARPAGMFVKKAAEFKSTVEVVAKDKTVNAK
                     SIMGIMSLGLAQGEELKLVVNGEDEEVALAAMVELVESGFGE"
     gene            36346..38058
                     /gene="ptsP"
                     /locus_tag="PC414_000376"
     CDS             36346..38058
                     /gene="ptsP"
                     /locus_tag="PC414_000376"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDF2462791.1"
                     /translation="MAYKGIGASPGVALGKALVVEHSELVIEKKSIDNVEAEIAKLED
                     AVAVSKEELVKVKEKASEELGAEEAEIFEAHLLVLEDPELIGSAIDKIKTESVNAEYA
                     LNEIKEMFVSMFESMDNEYMKERAADIKDVTNRILRHILGIKVVDLSALSEEVVLIAH
                     DLTPSDTATMNKKMVLGFLTDIGGRTSHTAIMSRTLEIAAIVGLNDITSKVKDGDFVV
                     FNGDTGEVIVNPDEETINKYTELKAKYEDERKALQLLKGKPSVTLDGKHVELAGNIGT
                     PNDIEGLIKNDAEGVGLYRTEFLYMDRDSFPTEEIQYEAYKAVLEGMDGKPIVIRTLD
                     IGGDKELSYLSMEPEMNPFLGYRAIRLCLDRKDIFKTQLRALYRASVHGRLRIMFPMI
                     SSLEELLQAKEVVKEVLAELDSEGVAYAKDVEIGMMIEVPSAAVISDVLAKHVDFFSI
                     GTNDLIQYTCAVDRMNQKISYLYNQFNPAVLRLIKTVIDNAHKEGKWAGMCGESAGDQ
                     KMIPILLGMGLDEFSMSPISILPARKLITSVKESDMKKLADDVLNMGTAEEIKSYIEK
                     TFNI"
     gene            complement(38850..39515)
                     /locus_tag="PC414_000377"
     CDS             complement(38850..39515)
                     /locus_tag="PC414_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2462792.1"
                     /translation="MKGNIVQYNFADIEEEVYSLDYAIAWNTDEENVNIIPFTNKFCK
                     ESIESFCLGKINNFVEILNEGFVENHHYVHLDKMISVPKKKVNLVYQQDTHGYLLRDD
                     NDNLIPAKITSEQSKSISSKMELFCAGEEKCLINILLKADPSYILDVDSIKDKNILNL
                     GYESIDRYKEYNFDDDKILIFFINKKRYSVIMKKTNNSDNDLVSRNNAIKELFTNKAG
                     NLN"
     gene            39859..41289
                     /locus_tag="PC414_000378"
     CDS             39859..41289
                     /locus_tag="PC414_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HDF2462793.1"
                     /translation="MDIKNKIKSIFLPEKNDYLDDEEFEYDIENNEEDLKEDNKVHLN
                     DLSRVKYDYRELKDIENQTEEMCLLAVKQDGTIIEFVKDKTYKVCMEAVKQTYKSLKY
                     ITNQNEDICIEAVKQNYRALYYINNKTENVLIEAIKNASTYDVMEVFKFVEEQTEDVC
                     LAFIERASKNDVAEILKGIKEQTPAICLEAVKKDGKSLAYVKEQSNSICLEAVKENYS
                     ALSCVKEQTEEICIEAVKQNDFALYYVNEQTEKICMEAVKRSYMALQYVNKQTEEICL
                     EAVRIDGRALQYVKEQTEEICLESVRQNGKVLQYVKKQTENICIEAVRGSFEELEIKE
                     ILSYVKIPTERIFVEAVKQNGKILKYVENQTELICLEAVRENYNALAYVKEQTEKICL
                     EAVNQSYEALKYVKEQTEEVCLKAVKQDYRMLKYVNNQTEKICLEAVKQNYRALEFVD
                     NQTEKVCLEAVKQNRKALQYVKQKQS"
     gene            41657..42235
                     /locus_tag="PC414_000379"
     CDS             41657..42235
                     /locus_tag="PC414_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HDF2462794.1"
                     /translation="MFKRYAEKMTSVLICNNMIDNNESKVYSYGFEILIAFIVNITTM
                     LFIGFLFGKFTYVLFFLMCYCPIRQFSGGYHADNYFRCLLTFIFIILSTILIIENINI
                     DLFKNIIMIIASVSWVGICVLCPIEHRSNPISDREKLVYKKTAIFISTVVLLITISSL
                     SLSIFVDYFTYSAFAMFWIFVMLVLGKLKAKV"
     gene            42265..42411
                     /locus_tag="PC414_000380"
     CDS             42265..42411
                     /locus_tag="PC414_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HDF2462795.1"
                     /translation="MKKFIVRFMKFASSLALSTAILSANSTCPWIIHQPKVPKEISNL
                     KKTN"
     gene            42707..44086
                     /gene="scfB"
                     /locus_tag="PC414_000381"
     CDS             42707..44086
                     /gene="scfB"
                     /locus_tag="PC414_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HDF2462796.1"
                     /translation="MSMIHKFSMNGYNIVLDVNGGAVHVLDDVAYDLLDFYKEKSKEE
                     ILEILKSKYQEEKINEAYEEILNLEKEGLLYTEDTYQYHPSFVHREPVVKALCLNVAH
                     DCNLKCKYCFAAQGDFGGEKELMSFEVGKAAIDYLIANSGSRKNLEIDFFGGEPLMNF
                     EVVKQLVDYGRSVEKDYNKNIRFTITTNGVLLNDEIIDYINENMHNVVLSLDGRKEVN
                     DNMRPTLNDKGSYDITLPRFKKLVEKRAKDKYYYIRGTFTRDNLDFSKDVMHFADLGF
                     KLTSVEPVVGDESNPYALREEDLPKIFEEYEKFAVEYADRQLQGDGFKFFHFMIDLNQ
                     GPCVIKRITGCGAGNEYLSVTPNGDIYPCHQFVGNEEFKMANIFDEEIVLPENLKNMF
                     REAHVYTKEECKQCWNKFYCSGGCHANAINFNNDISKPYELGCEMQRKRTECSIMIQA
                     KLMLEGATN"
     gene            44115..44612
                     /locus_tag="PC414_000382"
     CDS             44115..44612
                     /locus_tag="PC414_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HDF2462797.1"
                     /translation="MIRDYLEDKPLIDESVFVAKSADVIGNVKIGKDSSIWYNAVVRG
                     DEGPITIGENTNIQDCSIVHGDTETIIGNNVTVGHRSIVHGCKISDNVLIGMGSIILD
                     NAEIGEYTLIGAGTLITSNKKFPPGVLIMGSPGKVVRELTEEDKKYIDESYEWYLEAA
                     QNQKY"
     gene            44910..46574
                     /locus_tag="PC414_000383"
     CDS             44910..46574
                     /locus_tag="PC414_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HDF2462798.1"
                     /translation="MRISYRNLKRYREIGYVLIKYGFSFIVERLNIEGIAYKIPLFDP
                     PEEIKNMTTGERMKRVLEELGPTYIKIGQILSTRKDLLDQDIIDEISKLRDDVEKFDS
                     NIAIDIFKEEVGLSIEEIFLEFKEEAIAAASIGQVYEGVLKTGEEVIVKIQRPNIEKI
                     IKSDLEILRTIANTLKDLKKDFNLDLVQMIEEFQTQLMRELDYTFEAINATKFSRIFK
                     NSDEVYIPKVYSEYNTKKILVMEKVNGTKLSDVEKIRRLGYNTKTIVEIGVRSFFTQV
                     LSHGFFHADPHPGNIFVVAKNKIAYIDFGMIGIIDNKTLNQLNEIALAGVEKNVDKII
                     YLLIEMDALNGEADIKGLRQDLLYLIHYYYDISIEKINVTDILNELFRFFRQYKIVMP
                     AQFVTLAKTVITLEGTSRTLNTDFSFGSMGKEFMKHHYKSKFNPKNVVLSSRQNVEEI
                     LLDIKTIPKQLKAILKNIERNNIKMQIEDVKMTRLENCIIELTSQISLSLVLASIIVG
                     SSLIIASPNIENNIWIKFTAIAGFFISFIIGLCLVIRSIRSKYKKD"
     gene            46587..49037
                     /gene="recJ"
                     /locus_tag="PC414_000384"
     CDS             46587..49037
                     /gene="recJ"
                     /locus_tag="PC414_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDF2462799.1"
                     /translation="MKKWILKYKGKIEESEFSKKLNISPEICQILKNRGICTEKDSEI
                     FMNPSLDYLRDPFLMKDMQKAVDRIKLAVEKNEKIWIYGDYDVDGVSSTSILCLYFKS
                     IGYNVDYYIPNRLEEGYGINEDAIKLISSRGCDLIISVDCGITSVSEVNTANDLGIDV
                     IITDHHECQSEIPSAFAVINPKQEDCNYPFDSLCGCGVAFKMIQALTPKEEFKTSMYN
                     YLEIVTLATICDIVPLIDENRIIVKNGLKSMKEGKNIGLRELIKVCGVESDKIGSSHI
                     GFAIGPRINASGRLGYSYLGVELFTTQSQEEAVEIASILEEKNNERQMIEAKMYHEAE
                     EMLKSNSRYNDDKVLVLAKEGWQHGIIGIVASKLTEKYYKPTILLGIENGEATGSARS
                     IKGFNIFEALIKCKDLMTKFGGHEQAAGLSLDSDNVEILANEINKFADYNLTEDDMIE
                     NVNVEFELQENVINLNLVEELHKLEPFGLNNPNPRFIVRNYILKDLKVIGKNQQHLKL
                     SIEKEKSYECIGFNMSHLKSMYKVGDKVDVLFQLDENNYMGNRKVQFLLKDIRLARPK
                     SASNDKLSLKLMSKIIPKDTQSLYNISVSDFELFDGNTDINIFDYFEKDTLIISNSIN
                     GFYRAMSDISLIDLDFNINYNIIEDVSKNTDKLELIFSPNIDKIDLKRYNNIILYDYL
                     YNKGEYSYIYENKREESEIIKYYNKTDLLYLKNVVSNIVPSRDEFITIYKQALIKKEI
                     DLDMVNIRETFNVIPLKFFTILNVFRELNLLDFNLNYEKNSVLIRILPKPQKKLDLNE
                     SLILNNLKNLEKQYNSSY"
     gene            49050..49562
                     /locus_tag="PC414_000385"
     CDS             49050..49562
                     /locus_tag="PC414_000385"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDF2462800.1"
                     /translation="MDLKNFIRNIDDFPKPGIDFKDVTTLFKDGDAFKYAVDSIVEEL
                     KDKDVDLVIGPEARGFLMGTPVAYALGVGFVPIRKPGKLPGEVESYEYGLEYGTDTLE
                     IHKDAIKKGQKVAIVDDLLATGGTMEAAAKLVEKLGGEVVSMQFLIELKFLNGREKLS
                     NYDVNSLIKY"
     gene            49636..51843
                     /locus_tag="PC414_000386"
     CDS             49636..51843
                     /locus_tag="PC414_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003229747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HDF2462801.1"
                     /translation="MHDKELQEIIEKIKVYAPNANFEMVEKAYNLAKKAHEGQYRKSG
                     EPYFIHPLAVANILIEMELDIETITAGLLHDVVEDTEYTYEDIKKEFGKEVADLVDGV
                     TKLGQIKYRSKEETQSENLRKMFLAMAKDIRVILIKLADRLHNMRTLKYMPPEKAKYK
                     AKETLEIYGGIAHRLGISKIKWELEDRALRFMDPEGYYDLVSRVSMKRSQREDYIQGI
                     VELLKDKFKDLSIPCDVYGRPKHFYSIYRKMQKKHKTFEEIFDLTAVRILVDNVKDCY
                     AVLGIVHTLWRPIPGRFKDYIAMPKPNMYQSLHTTVVGPDGEPLEIQIRTHEMHNIAE
                     NGIAAHWKYKEGISSKESKVEEKLQWLRQMMEWEKDLKDPQEFMDALKEDVFNSQVYV
                     FTPKGDVIELPAESTPIDFAYRVHTNVGNKCVGAKIDGRIVPIDYKLQNGNIVEVLTS
                     SNSNGPSRDWLNIVKTPNAKSRIRQFFKKERREENVDRGNTILEREFKKHDIPQKDPL
                     VEKYMALVAKKFNQPSVEDLIATVGYGGIMSSQVVSKVKDLYLKEVKKAKKEQQIQEE
                     DLNKHNISETEYSKKRKKNASQGIIVKGLDNILVRFAKCCNPLPGDEIVGYITKGRGV
                     AIHRADCPNANNNGDFFQNRLVEVSWSSAGKGKFEAEIQIKAIDRRGIVNEITHIVAM
                     DKLSLNGINARKGKDSIVSINLLVEVNDTEELKILMKKLKAIPGVESIYRMTN"
     gene            51868..52317
                     /locus_tag="PC414_000387"
     CDS             51868..52317
                     /locus_tag="PC414_000387"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HDF2462802.1"
                     /translation="MRAVVQRVSSSKVTVDENTIGQINKGLLVLLGVTHDDKSSDVDY
                     MIDKILNLRIFEDENDKMNLSLMDIGGELLVVSQFTLYGDCRKGRRPGFSNAARPELA
                     NNLYEEFVKKAKDKGVTVGTGQFAAHMMVELTNDGPVTILLDSSKSF"
     gene            52332..52946
                     /locus_tag="PC414_000388"
     CDS             52332..52946
                     /locus_tag="PC414_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2462803.1"
                     /translation="MIIKKFVDSYFGVNTYVLGDEKTKKCAVIDPGGSLVETLSFVKE
                     NELNIEYIILTHGHGDHIGYVKDIKEKTGAKVVAHVDEKELLNDKNKNLSYTMRCGAQ
                     EFDADIYVNDKDKLELGELKMTFLHTPGHTQGCMCIRVEDEMFTGDTLFAGSIGRTDL
                     YSGDFNQMEKSLKKLCKYEDAVRVYPGHGPTSTLGVEKKTNPYM"
     gene            53037..54593
                     /locus_tag="PC414_000389"
     CDS             53037..54593
                     /locus_tag="PC414_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HDF2462804.1"
                     /translation="MLYVFLKGHDYKYEVAELIKLFTSEFKFKDSNANNNIESKHLIN
                     RLIYENGVLFSSTEYYENGNLKYESIQNIENMNLEFLDGFDSSKTIEEFLNSLDSESK
                     RNFKKLCKENIKKSMFLVLKQVFNSYVPWGILTGIRPVKIVHNLMDKKLDDNSIRTIL
                     KDNYFIIDEKIDLALEIAKRERLFMYPIDKNKISLYVSIPFCPTRCVYCSFPSNSLKQ
                     FGHLKREYVYKLIEEIKGFAKVIKDTKKEVETLYIGGGTPTTLDEEELDLLINSLFNE
                     LDLSKIKEFTVEAGRPDTITEQKLRVLKKHNVSRISINPQTMNDDTLVKIGREHRVSD
                     LVDCFNMARRMGFDNINMDIILGLIDEDLNMVKNTLEEIKKLSPESLTVHTLAIKRAS
                     NLNINMDRYKEHLTQYEEMVKMIDLSMKYAKDMGLNPYYMYRQKHMLGNLENIGYAKE
                     GYECIYNIQIMEEKQSNYALGAGAISKFVYVDEDRIERVENVKNVEQYIERVDEMIKR
                     KKEEVYKNVD"
     gene            54583..55845
                     /locus_tag="PC414_000390"
     CDS             54583..55845
                     /locus_tag="PC414_000390"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDF2462805.1"
                     /translation="MLTKAPRGTKDITPKEAYKWRYVENKFREICALYGYEEMVTPIF
                     EHTELFKRSVGDTTDIVQKEMYSFKDKGDREITLKPEGTAGVVRAFIENKLYADTQPT
                     KLFYVTPCFRYERPQAGRQRQFHQFGIEALGSDTPSMDAEIIALAVQFFNEVGLNDLV
                     VSINSVGCPVCRKEYNALLKEYLDSKADILCDTCNERREKNPMRVIDCKNPTCKENIK
                     DIPFIADHLCDDCKSHFDKLQEYLKEMNINFVIDKTIVRGLDYYRKTAFEIISNDIGA
                     QSTVCGGGRYDGLVEQLGGPKGISGIGFGLGIERLLLTLEGNGIEIENPQSTDIFIVT
                     IGEEANTRSFKLLKDLRQNHISADKDHIERSVKAQFKYSDKINSKFTIVIGDDELKND
                     TATLKNMKTSEQTTVKLSTLVEELKQKL"
     gene            55919..57706
                     /gene="aspS"
                     /locus_tag="PC414_000391"
     CDS             55919..57706
                     /gene="aspS"
                     /locus_tag="PC414_000391"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDF2462806.1"
                     /translation="METLKGLKRTHYCGELREKNINEEVVLMGWVQKKRNLGGLVFVD
                     LRDTSGLCQIVFDTDVDKEAFEKAEKLGAEFVIAVKGKVCERQSKNPNMPTGDIEIFA
                     TELRLLNKSETPPIYIKDDDDVSEALRLKYRYLDLRKPSMQRNLKLRHKVMNITRNYL
                     SNNRFCEIETPFLIAPTPEGARDYLVPSRVNPGKFYALPQSPQLYKQLLMVSGMDRYF
                     QIVKCFRDEDLRADRQPEFTQIDCEMSFVEQEDVMSMIEGLLEAIFKEVLDVELALPL
                     PKMTYAEAMSKYGSDKPDTRFGYELTDISDVVCNCGFKVFADATQPGKSVRGINVKGK
                     ADDFTRKQISSLEEHAKTYRAKGLAWMKVGQEGVTSPIAKFFNEEEMNAILTRMNAEV
                     GDLLLFVADKNSIVFDALGQVRLEVANRLNLLDKNVYNLLWVTEFPVFEEDEETGTFS
                     AMHHPFTSPMDEDLDKLEEGDKSSLRAKAYDIVLNGYEIGGGSVRISNSDVQSRMFKA
                     LGFTEERANEKFGYLLEAFKYGTPPHAGLAFGLDRLVMLLAGTDNIREVIAFPKNQNA
                     VCPMTNAPTLAEDEQLEELSIKVDIKDNE"
     gene            58165..59397
                     /locus_tag="PC414_000392"
     CDS             58165..59397
                     /locus_tag="PC414_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HDF2462807.1"
                     /translation="MQEREELVLNPVPKEERVGWLAPLFNMLGSNIAISELMVGGTLI
                     LGMTLSNMIITSIIGNLILVAIIMIQGYIGYKEGLNTYVLAKGAFGEIGGKYLISLLL
                     GITSFGWFGVQAGVAGLSVQKIFPSVNLTLVTVILGLFMVVFALYGFKAMAKFNYLVI
                     PPLMILMVWGVFKAFSTYGVEAIYNYTPQTTMSMVEGLNIVVGLVIVGAIISPDQLRY
                     TRRVKDIWIISFLGLGIISLFQQVAAGVMSMGAPTWDITEVLANLGFGWVAFVILILA
                     SWSTNVSNAYSGGLALKTIFPNVKRNVLTLVAGLIGTIIAATGIIFKFQSFLSFLGIA
                     VPAIAGIMWCEYYFIQGRTYKHREGINWIAVISWLIGFAASYYSSKINFLIPPINGIV
                     VSMVIYYILMNCFGIKDK"
     gene            59411..60217
                     /locus_tag="PC414_000393"
     CDS             59411..60217
                     /locus_tag="PC414_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HDF2462808.1"
                     /translation="MGRKVKIGIIQQHSVLGNVKKNIEKAVEMIDDLGKQGADIICLP
                     ELFATGYNLESLGGVKTLELIREHNKYIEESMSEAAKRNNVYLISPYGTLEKGSTHVY
                     NSAVIFDRKGKIMGEYCKNHLWSLEAVYFKGGEKVEVYDADFGRFGVMICYDAGFPEV
                     SRELTLKGSEIIFIPSAWRIQDEDMWDLNVSQRALENTVYTVGVNLVSNDSNLILFGK
                     SKICNPRGTVITQLDTYREKYVLSEIDLDEIKKYREDIPYLKDRKSSYNI"
     gene            complement(60329..61198)
                     /locus_tag="PC414_000394"
     CDS             complement(60329..61198)
                     /locus_tag="PC414_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2462809.1"
                     /translation="MDLLHLKYFQTVARMEHITKASHKLNIAQPALSKTISSLEKELG
                     VQLFDRKGRYIVLNEYGRLFLKRVDSILDLVESSKKELQDTSLENSGEVKILSPAAAN
                     VLPSLLSNFRKLYPNITFNVSHTLPSSYKKSDFDLYISSSFTKLNSENSITLTCEEIL
                     LGVSINHPLSLKDEVYLSEVSDENFIVITKGENYREVIDILCESANFKPKIAFESDSP
                     YTIYALIKSLQGVGFICGKSWGLSQDPEIKLLHIKDIEFKRYLNLSWFSENYESKAVL
                     LFKNFLINYFKNI"
     gene            61570..62997
                     /locus_tag="PC414_000395"
     CDS             61570..62997
                     /locus_tag="PC414_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp14"
                     /protein_id="HDF2462810.1"
                     /translation="MKLTKSISSMLCLSLVLSLSPVAYANEISRTTVENTLVGQDRYQ
                     TAVEVSKKGWSSANEAVVVNSESIVDALAVTPFAKLKNAPILLTEKNNLNSQTEKELK
                     RLGVKKVYIIGLSSSVSNDVQKQLESNNLTTDRVGGSDRYQTALSIAKKIEGLKDISE
                     IAVVNGYTGLADAVSIASVAATNGMVILPVSDASGISSFKDFIESKNINKSYIIGSTN
                     AVSDKIKQSLPNSERIGGADRNETNGKVIEKFYTSNKLNNVFVAKDGMKNQNQLIDAL
                     AVGVLASKENSPVLIVGEQLSATQKSVFSNKNATSITQVGSGGNENAFKELKDLQANS
                     NNKRVMYVTNTDKVNIRNDATIEASVIGYLNNGDEVEVLDVLKTGWVEIKYNEGIGYV
                     SGSYLTNNKPDNSNENIKIKYVKEKDGLNVRKGPSTEDEKIGHLSYGSKVETIEMFAT
                     GWVKIKYNGGYGYVSNDYLSDTPVI"
     gene            complement(63074..63343)
                     /locus_tag="PC414_000396"
     CDS             complement(63074..63343)
                     /locus_tag="PC414_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2462811.1"
                     /translation="MKFEYYYLIQDIAGILLAFIGLRMSIIGFRILSMKGLSINTLLI
                     VIKYCLFTIAGLNLLISKFGIRHWIWSVCMLIISIIINPRIKVSK"
     gene            complement(63346..64788)
                     /gene="nhaC"
                     /locus_tag="PC414_000397"
     CDS             complement(63346..64788)
                     /gene="nhaC"
                     /locus_tag="PC414_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDF2462812.1"
                     /translation="MKMNTERKIPSFRYSLLVMLAIIALTSVGIVVFNASITTMFLLS
                     WLIVVPAAMKLGYTNDEIEAFGFEVGKDAFQSNLIILSVGVLIAAWIAAGTIPTVVYS
                     GLTIITPKYFLLTTLIVCSLTSVATGTSWGTLGTSGIAMMSIGTSMGIPLGLTAGAVI
                     SGAFFGDKMSPLSDSTNLAAAVCKTDVITHMKHMFYTTGPAYVICIVLYTVIGFKYSN
                     NTIDYVQINQIKDVLNSNFHIGLVAMIPIIFLLLLLLLQKPPIISILSSAIMGLIIAV
                     FQEGEKIGDVLNYMLSGFTIDTGFVYADKLLNRGGIMSMAETVLLVFVVFVIAGILQK
                     TGFLEVLLQPLITKIGKSRTKLVGSTFIVSYFANAFSSSMMFTSVFVGTLMSPIYKEF
                     KLKPQNLSRIIEDTATLGGPLIPWNSNAVFCAQTLGVSPLEFIPYCFLSWITPIISFI
                     YGVTGFTMLTYTDDEIEELEIFDTAQRRVD"
     gene            complement(64813..65979)
                     /locus_tag="PC414_000398"
     CDS             complement(64813..65979)
                     /locus_tag="PC414_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2462813.1"
                     /translation="MNYNFNEIVDRSNNFSSKWSEMEKKYGTNDLLPMWVADMDFKAA
                     PCIIDSLKNRLEQEIYGYTTRPDSYNESIVNWLDRRHNWKIKSEWLIYSPGVIPAISL
                     LINELTKANDKIMIQEPVYSPFNSVVKNNNRELIISPLQKLENGNYIMDYEDIENKIK
                     DVKLFILCNPHNPVGRVWTKDELKKLGDICLKHNVKIISDEIHSDIILKKHKHIPMAS
                     ISKEFEKNTITCMAPTKTFNIAGLQSSYVILPDEKDYKLLDDAFTRIDIKRNNCFSLV
                     ATEASYNNGESWLESFLEYLESNIDFAIKYINENMPKLKVRKPEGTYLLWVDFSALGL
                     SDEELESILVQKGKVALNQGNSFGIGGSGYQRINLACPRSMLEEALIRIKNAIN"
     gene            complement(66204..68324)
                     /locus_tag="PC414_000399"
     CDS             complement(66204..68324)
                     /locus_tag="PC414_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2462814.1"
                     /translation="MKQMELAIVSLKKDAGEIYENQIRQFLGDNLKINLYSFEEGNLK
                     FFKEKLILLSAYLKYDEIVKLSHYDAQIIVPKLTFEKNSIDMISKLEKDKIIYVYNLS
                     KDMAIETISLIHRLGIDNINILPCYPEIEFTPTDAVILTPGEKILPKFKNCEVIDLKY
                     RIIDLSCIVEIATKTKLKHLIKDDLIKKYVEKIIPTSYSTGELLLDANKFERQFDLLL
                     SIIDDGIICTNNDGIIQFYNHMARKILSINANEMIDSFVGDCIKDINFQSILTNKTPF
                     FEKLIKINHIDINLEIKHIQLNVFDGFILKMTKFSQLEKKQAKLRAQLVNSGNISKYT
                     FDDILGSSIQTINTKKIANKMAQSNSSILIIGESGTGKELFAQSIHSASRRKDGPFVA
                     VNCSTFQENLLQSELFGYDEGAFTGAKKGGKIGLFELANNGTIFLDEIGEMDLNSQSK
                     LLRVIQEKQVRRIGSNNVIDIDVRIIAATNRNLKELVSKNMFRRDLYFRLNVLPLKIH
                     PLRERPADIFEIFGSLKYDIPCNFILSEEVKEIFKMYRWEGNVRELRNLGEYFCYLEK
                     DIIEICDLPEYILDTIDSNYSRTVCNKVSDNIKKYQFNIGKDKNIMKYDYNFKRSLDE
                     YIFILDNLKKAYDLKERIGRKSLCKIALEENRFLTEQQIRNMLLELQNFGLVDILVGR
                     GGSIITSKGVEFLKNINRSNKLNS"
     gene            68557..68958
                     /locus_tag="PC414_000400"
     CDS             68557..68958
                     /locus_tag="PC414_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2462815.1"
                     /translation="MKKCLYCNRDIKSPNNKLAIKYDDNMNIYPCRAECKEKIEEYIA
                     KKPTYKFINILEVLLGVGGIFCFLSKWTIAAQVVLGIDALVIFLFPLAGFKMNGKLSM
                     EQTKNQSRSIAISILAFIVIITVLYFKQVGK"
     gene            69001..69294
                     /locus_tag="PC414_000401"
     CDS             69001..69294
                     /locus_tag="PC414_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HDF2462816.1"
                     /translation="MEERKLAPNNEREALIKRLKRIEGQVKGIQKMVEQERYCVDILV
                     QISAIRSAINRVGTIILENHIKGCVAESIKHDDADKTEEMIKELMDTINKFTK"
     gene            69309..69749
                     /locus_tag="PC414_000402"
     CDS             69309..69749
                     /locus_tag="PC414_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HDF2462817.1"
                     /translation="MNQQGFIMEIVDDRTAKLKMQRHSACAACGKCATTNSAESKEIL
                     VEVDNTIGAKVGDHVEVSMDNMNVLKAAVMAYIIPLIALLVGTIGTYYILGAIGMTTG
                     VEAISGLVGICLTIICYLYLKKNDKKFRDSREFIPVITRILIDL"
     gene            complement(69841..70314)
                     /locus_tag="PC414_000403"
     CDS             complement(69841..70314)
                     /locus_tag="PC414_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDF2462818.1"
                     /translation="MTDMSMILHFLFSGVATAGFAVFFNAPLYLLLPAGVTGGIGWIV
                     YVYLFNFTTNAVFAGFIAAALVSACSETLARKLKQPAIVFVIPGILPLIPGIGLYNTM
                     LSLIQKNYSLAMSKGTDALFLSAAIALGVLVVTSFVRTLNLLKIRKNFIPYRKSK"
     gene            complement(70314..71102)
                     /locus_tag="PC414_000404"
     CDS             complement(70314..71102)
                     /locus_tag="PC414_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDF2462819.1"
                     /translation="MNQNEELEYKKNILKLALFVGELMIKNGAETSRVEDSVLRICRS
                     RGFYHVNIFTTPTVIIISDEKFDGFSFMKTIQSRGINLNKISLLNSFSREFVSDKEYD
                     IDNAIARLYEIQDVKPYPLWLFYSCTGIASACFACLLGGNYVLNFILTFIIAIFAAIT
                     YDKTMKISAISAFSCLVSSFLIALSGVLLVEIGILDDPKMLIVGAIMPLLPGVAFIKA
                     IRDLISGNLISGISRAFDAAMIIIAIASGVGFVLDTWYRIGGPF"
     gene            71430..72674
                     /locus_tag="PC414_000405"
     CDS             71430..72674
                     /locus_tag="PC414_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDF2462820.1"
                     /translation="MFENLKKADPEIYESMKRELKRQQRNIELIASENIVSVPVMETM
                     GSHLTNKYAEGYSGKRYYGGCEYIDEVETLAIDRIKKIFGAEHANVQPHSGANANIGV
                     YFAMLKPGDVVMGMNLSQGGHLTHGAPVNISGQYYKFYEYGIDKDSGLIDFDEVRKIA
                     HEVKPKMIVAGASAYPREIDFKKFREIADEVGALLMVDMAHIAGLVAAGLHQNPCEVA
                     DFVTTTTHKTLRGPRGGVILCKEKYAKDIDKAIFPGIQGGPLEHIIASKAVCFKEALS
                     DEFKEYQVQVAKNAKALAEELIKRDFKLISGGTDNHLILLDLTNKNITGKAAEKRLDD
                     AYITANKNTIPFDPNGALITSGIRLGTPAVTTRGMKEEDMAIIAEAIDLCLTYDEESK
                     ARTLVVGLTEKYPLYEEYNIMD"
     gene            72997..74094
                     /locus_tag="PC414_000406"
     CDS             72997..74094
                     /locus_tag="PC414_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-diphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDF2462821.1"
                     /translation="MKVLILTGKFGMGHYSASNSLSEDIKAKFDNSEIIIKDIFEYIM
                     PNYSDKMYKTFSILVNRGSSLYNLFYKCAENGKKDIKFTFSDYFLNKLDTLLHEVQPT
                     VVISTFPFCSQLVSRYKEKYNSNLPLITCITDISSHSEWISKNTDCYLVASKSTKEEL
                     VFKGIDESKIKVNGIPVKKEFKRIEHVNHSTKKNILIMGGGLGLLPKSEQFYKELNSL
                     EGVKTTVITGNNKKMYYKLYGRYENIEVVGYTNEVYKYMKDSDLIISKPGGITLFETI
                     YSELPILAFNPFLQQEIDNASFILNNEIGRILGKNKKYYVDEIKDLIYDDATLKEMSS
                     NMKELKKQFDNNTLENILFSLDEQGACRECM"
     gene            74085..74783
                     /locus_tag="PC414_000407"
     CDS             74085..74783
                     /locus_tag="PC414_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2462822.1"
                     /translation="MYVVGLIIVVALIFLVHSIIPTYYNKLLNKEVLKNMAGENEIAL
                     TFDDGPDKRYTEKLLDVLKENDIQAMFFVVAKNAEKEPEIIKRMLRENHIVGLHSLEH
                     RNAWLYSYSYVKKDFIESTNIMKNLGVDVNYYRPPWGHTNIFSNSFVKKYNLKMTLWD
                     VMAEDWEKDSTVDIIINKLMSRTKENSIICLHDAGENSGGAVGAPERTIEALKIAIPK
                     LKASGLKFVTPERM"
     gene            74786..75850
                     /locus_tag="PC414_000408"
     CDS             74786..75850
                     /locus_tag="PC414_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HDF2462823.1"
                     /translation="MISKTEKKNKCLGSVAFLVLLMGITGYFVFRGQSVESLIKSLKG
                     ASPMFILIGFAMMFIYVACEGINIYLGMKALNQKTTLLKCMGYAFIGFYFSSITPSAS
                     GGQPAQVYYMKKDDINISYSSLILLVIVVIHQVVILAYSGIMFIMEREFILNNVSGMN
                     ILLIYGVITNVALVIGVIAIIFSKKLVNNFIISITNLLGKLRIIKDVESSRKVIISQI
                     EEYVKGAQYIKQNPKLVVQILVITIVQITAMFLVPFFVYKAFHLSTYTVFEILAIQSL
                     LNIAVSSLPLPGAVGASENSFMTLFKIFFPGHLLVPAMLLSRGISFYAFVAISGLICI
                     VVHVKSSEKVKAVKKVAYVR"
     gene            76033..76737
                     /locus_tag="PC414_000409"
     CDS             76033..76737
                     /locus_tag="PC414_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2462824.1"
                     /translation="MNKEIPKKILVADDNPEIREIVEILLTGEGYEVIMATNGEEAVN
                     LVDSTIDLIILDVVMPVKTGFVACNEIREKTTAPILFLTAKTQDSDKVIGFSAGGDDY
                     LSKPFSYSELISRVKSLLRRYYIYQGKEKEREPNILKTKELCVNLDTQDVTLNGEPVF
                     LTTIEYSILILMLKNRKKVFSSENLYETIWDEQYFYTANNTIMVHIRNLRKKLEKDQK
                     NPQYIKTAWGKGYYID"
     gene            76730..78052
                     /locus_tag="PC414_000410"
     CDS             76730..78052
                     /locus_tag="PC414_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2462825.1"
                     /translation="MINKIKPSKLGVILLIYVVISAAVAFILNIPFYSAVESIVKDVS
                     EGKVYFKREVNQLMDNFQTYVNNNGIRADDGDDIAKWNSDNWFVSMKIYKGNKVFYDT
                     LYYPNEVPDKSEENEFYSSPMARNYEIQFSDIKANIVVVALFRFRFQNFIEAISITFM
                     ALTFIITFLLLFSRKIKYLRKIEMGIKIVESGSLKYRIPVKGNDELSSLASSINEMSH
                     TLENQLIKEREMKNKNKDIVTSISHDVRTPLTSVICYLDLIKDHKYNSPEQLEQYIKN
                     VRSKAYQIKDLTDDLFTHFVNSGEEVSKGYEKINANEFIFQLLLDSSYTLEEKGFHIT
                     IENELYNQYHIYVDVTQFRRIFDNLCSNIIKYARKDKPIVFLIKVEDSKLLIIQKNRV
                     SNFSKNVESFGIGIKNCEKIVAYHNGSINVNSDDKEFAIEISIPAKES"
     gene            78239..79612
                     /locus_tag="PC414_000411"
     CDS             78239..79612
                     /locus_tag="PC414_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2462826.1"
                     /translation="MVLTGAHYIYILFMVIILITMIMKKDTIVPCILGVFFMGLFFEK
                     NIFGAITAVFNSFIISLNELGPIILIIAIMVALSKALEANNAIQYMVRPFSRVIKNSN
                     TAFFVTGFVMLVLSWFFWPTPAVALVGAVFLPVAMRAGLPAIGVAVALNLFGHGLALS
                     TDFVIQGAPSITAGAAGVAVSYVINDGMILFWVMGIVTISMAFYTLKRDINKGMFREE
                     LKGFESEEVEEFNGKSKIATILVALGFLADIIAMYIFDLKGGDASALLGGTAVFLIII
                     INVINFGKDSLENVCENIIDGFVFGIKIFGAIIPIAAFFYMGEVAPLTGVFGKVLAPG
                     SQGLLSDIGIALSQTIPLNKFAVSGIETVVGAITGLDGSGFSGISLVGSLASVFGTAI
                     NASVGALAALGQISGIWVGGGCLVPWGLISAAAICGVSPIELAKRNFVPVITGLVVTT
                     IVAMFII"
     gene            complement(79984..80922)
                     /gene="pdaA"
                     /locus_tag="PC414_000412"
     CDS             complement(79984..80922)
                     /gene="pdaA"
                     /locus_tag="PC414_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HDF2462827.1"
                     /translation="MFRKVLHYLTLLSISIFFIVGCSNSQNNQNENQNKETQLQEDKE
                     KIDSGKDTSNVIVSDGTDKPSKATTNNDNNKLDVSSLDNTTLDWFYIPNNKHKTPEVN
                     TDIEFKFSDYDALYNGPTKDGQKTLYLTFDEGYENGYTTKILDTLKQNQVKAVFFVTA
                     PYIKENKDLVKRMVSEGHIVGNHSKTHPSMPTKTSNLKNFNDELYDVEKLYKDVTGKD
                     MVKFFRPPMGKYSEKSLAMTKNLGYKTVFWSFAYRDWDTDKQPSHEEATQKIMDNLHD
                     GSILLLHAVSKTSTEILNDFISNARKLGYEFELLEY"
BASE COUNT        31849 a         8878 c        13995 g        26473 t
ORIGIN      
        1 atttcaaata atgctgtaga ttctaaactt atgaatagta ggattaaatc aatagatata
       61 ataagagggc tatcaatagc cctcatgata gtctgcaata atcctgggac ttggatgaga
      121 atgtatcctc aattaagaca tgctgtatgg catggtgtaa cacttgcaga ttttgcattt
      181 cctttttttg ttatatcttt aggtgttaca attccaatat caataaatag taaactaaaa
      241 aataataaat ccacactgag tataatactt agtatattca agagaagtat cttattgata
      301 ttatttggat ttttcttaaa ttacctagga aatcctgatt tagatactgt aagaatactt
      361 ggagtacttc aacgtatggg attagtctat tttgtgacta gtttagtata tttactacta
      421 aaaaaattaa atgttggaag tactgctaca ataattactt ttctatgtat atctacattt
      481 ataatagttg ggtactacat attagcaaaa ccttatggat ttgagctaga aggttctctt
      541 gcacagttag tggatttaca tttcttcaag ggacatttgt acaagccgga atttgaacca
      601 gatggttttt taactagtat agtagcaatt tcttcaggta tgttgggatg cactatggga
      661 tgtgtacttt taaaagaaaa cataggagaa tataaaaaat tctttaaaat actggttatg
      721 agcataatcc tgctaatagg tgcttttatc tttaatcagt attttccatt taataaaaga
      781 ttatggtcta gctcatttgt acttttaatg gctggcagtt atggtatatt actgtcaata
      841 ttttatttta tatgcgacat caaaaataaa agtaaaatat ttaccccaat aatagcactt
      901 ggaagtagcc ctatttttac atatatgtgc cttgaaatat tgagtcatgt tttttggaat
      961 gttccaaaac ttacgaacaa agtcgattat ccaactacct tggtagagtg gactacatat
     1021 gaacttataa caccttgggc aggtacaact tgggattctc ttatattttc tttattgtat
     1081 gtcctattct gggttatagt aatgtctata atgtacaaga aaaaaatatt tattaaaatt
     1141 tagtgcaata tttttagatg ataaagtctt tgatttgttc aaatatacgg aaactatcaa
     1201 atataatcaa aatttctcct cgataaaatt taaaaaccat gccataaaag attacaaaaa
     1261 gtttacaatt tgtaattttt tgtgctataa tgaaaaaaga tttacataat attgaattta
     1321 tgtaatataa aacaagctta agatgtatta aaaaagattt aaagatttaa agaaccaaaa
     1381 aattaggggg gatatgatga aaaaaacaac aaaattattg gcaacaggaa tgttgtctgt
     1441 agcaatggtg gcaccaaatg tagctttagc ggctgaaaat actactgcaa atacagaatc
     1501 taattcagat attaatataa atttacaaag aaaaagtgta gtgttaggaa gtaaatctaa
     1561 tgcaagtgta aaatttaaag aaaaattaaa tgctgattca ataacattaa attttatgtg
     1621 ttatgatatg ccacttgaag ctactttaaa ttacaatgaa aagacagatt catatgaagg
     1681 tgtaataaac tataacaaag acccagaata cttaaatgta tgggaacttc aaagcataaa
     1741 aataaatggt aaagatgaac agaaagtttt aaacaaagaa gacttagagt ctatgggatt
     1801 aaatcttaag gattatgatg ttacacaaga gtttataata agtgatgcaa attcaacaaa
     1861 agcagtaaat gaatatatga gaaaaacttc tgcaccagta aaaaaacttg ctggagctac
     1921 tagatttgaa actgctgttg agataagcaa acaaggttgg aaagatgggt ctagtaaggt
     1981 tgtaatagtt aatggagaat tagcagcaga tggaattaca gctactccac ttgcatctac
     2041 atatgacgca ccaatattac ttgcaaataa agatgatata ccagaaagta caaaagcaga
     2101 gttaaaacgt ctaaatccaa gtgatgtaat aattataggt gatgatggtt cagtttcaca
     2161 aaaggcagtg tctcaaatta aatctgctgt aaatgtaaat gtgactcgta ttggtggagt
     2221 tgatagacat gaaacatctt tacttatagc aaaagaaata gataagtatc atgatgttaa
     2281 taaaatatac atagcaaatg gatatgctgg tgaatatgat gctcttaata tttcatcaaa
     2341 agctggtgaa gaccaacaac ctataatatt agctaataaa gattctgtac cacaaggtac
     2401 atataattgg ttatcctcac aaggtttaga agaagcttat tacataggtg gtagtcaaag
     2461 tttatcatca aaaattatag accaaatatc taaaatagca aaaaatggta cttcaaaaaa
     2521 tagagtttct ggagcagata gacatgaaac aaatgcaaat gtaattaaga ctttctatcc
     2581 agataaagag ttgagtgcaa tgttagttgc aaaatctgac ataatagttg attcaataac
     2641 agcaggacct ttagcagcta aattaaaagc tccaatactt ataactccaa aaacttatgt
     2701 ttctgcatac catagtacaa acttatcaga aaaaacagca gaaacagtat atcaaattgg
     2761 tgatggaatg aaagatagtg ttataaattc tatagcatct agtttatcaa aacacaatgc
     2821 accaacagaa ccagataatt caggttcagc agcaggaaaa actgtagtga tagacccagg
     2881 tcatgggggt tctgattcag gggcaacaag tggattaaat ggtggagcac aagaaaagaa
     2941 atatactctt aatacagcat tagcaactac tgaatattta cgttcaaaag gtataaatgt
     3001 tgttatgact agagatacag ataaaacaat ggctttagga gaaagaacag cattatcaaa
     3061 tactataaag ccagatttat ttacaagtat acattacaat gcgtctaatg gatctggtaa
     3121 tggtgtggaa atttattaca aagtaaaaga taaaaatggt ggaacaacta agactgcagc
     3181 atcaaatatc ttaaaaagaa tactagaaaa atttaatatg aaaaatagag gaatcaagac
     3241 aagaacactt gataatggaa aagattacct atatgtgtta agaaataata attatccagc
     3301 aatacttgtt gaatgtgcat ttattgacaa taagagcgat atggataagt taaatactgc
     3361 agaaaaagta aaaacaatgg gaactcaaat tggaatagga atagaagata cagtaaaata
     3421 aaatagaaaa tattaaagca aaacatatca tctcctgtta taatgtaaaa tataacaaga
     3481 gatgatatat ttttgtaaag aatcaaattg tttacaatga tgtttcttgt attgataatc
     3541 tgctcattat tgagtataat atagaaataa gtcaaaaata agatagaaat aagttgaaat
     3601 taagaattaa aaaaattttt attccaatat ggaggtaact atgtctggat atactaatga
     3661 tgagtgtgaa atacctaaaa ttaaaagcta tccaggggca gataaagaaa ttgcaagtga
     3721 gattgactat agcatagtaa aaggaacagt tttattcgac ttgtaccaaa gaattatgga
     3781 tttagtttta tctataatag gacttgtgat aggtttacct ttaatagcaa tatttggaat
     3841 tcttattaaa atagaggaca aaggaccaat aacttataag caagaaagac taggaaaatg
     3901 cgggagaaga ttctatatat ataaattgag atctatgaga acagacgcag aaaaatttgg
     3961 cgcacaatgg gctgaaaaag atgatcctag aataactaag gttggaaagt ttattaggaa
     4021 aactagaatt gatgagattc cacagctgtt taatatatta aagggggaca tgggcttaat
     4081 aggtcctaga ccagagagac cgaactttac tgttcaattt aatgaggaaa tacctggatt
     4141 tatcaacagg ctcgctataa aacctgggct tacaggttgg gcacaagtaa atggtggata
     4201 cgaaatcact cctgaagaaa aacttaaaga agatatttac tatataaaaa ataggagtat
     4261 attacttgac tttaagatac tctttaaaac tgttaaagta gtattgacag gagacggagc
     4321 tagataattt taaaatatta gattactaat caatgaaaaa gctctttaaa aagagctttt
     4381 ttattgtcaa aaaatacaaa aaagaaaagg aatgagaact tatgattaaa aaaatttcta
     4441 ctatattatc gttagttttg ttaatctcta tttctagtac gataggagta tttgcagatg
     4501 caaatcccaa aagagaattg atagaaggga gtataccaga gatttcgaca gaattaaata
     4561 aaagagcatt taaggattct aaagaagtaa ttcttgtaaa tgaagagtca attgtagata
     4621 gtataagtgc aacaccttta gcatatgcaa aaaatgctcc aatagttgta actaagagta
     4681 aaaatcttgg tagagtaaca agaaattatc taaaagaact aggtccagag aaggttacga
     4741 tagtaggtgg tttaaaggca gtatctaaag atgctgaacg taatatagaa aaaatgggaa
     4801 tgaaagtaga aagaataaga gggaaagaca gatatgatac atctttaaag atagctagag
     4861 aaatgtatag aacagtagga tttgatgaag catttttact tagctcaaca acaggattag
     4921 agaacgccat atctgtatat tcttatgccg ctaaaagtgg aatgccaata atatgggcta
     4981 aagatgaagg gtttgaggaa caaattgatt tcttaaaagg caaaaatctt aaaaaaatat
     5041 atgcattagg tgattcaaaa gagtttattg cagagattga ttctaactta aaaaatatag
     5101 aaggaataaa gcaaataaat aagtcaagta caaatgttga tttaataaag aagttttatg
     5161 atgaaaaaga tataaagaaa atctatactg ctagattaga ctttggtagt agatctgatg
     5221 taaacgaata tatttctttg ggagtagtat cagcaaaaga aaatatgcca atactgattt
     5281 gtagtgataa cttaagtcgt gcacaagata aattcttgaa agatagcaat ataaatgatg
     5341 tagttgaagt tgggtataca gtaggtgatt atagtctatt taagagcata tttaatctaa
     5401 cattcttatc ttgtatagta ttaatactat tattactatt gattacattt agggcactta
     5461 gatatgagtc gaaatagttt ttgatttata aggaagcttt tcataggtta aatttactta
     5521 attagaataa tgtttataag cagttttaaa agttctactt ttagagttta aattagataa
     5581 ggagaaagaa gatgtcaaaa acagctaaag cagcattgtg gattatggca gccactatgt
     5641 tttcaaaagt attaggtttc cttagagaac ttgtacttgc aaatttctat gggacaggta
     5701 tgtatgcaga cgtatttgta ttaacattaa atatacctgg tttaatcata gcagtcatag
     5761 gctctgcagt tgctacaact tatataccta tgtactttga gacaaagaaa agactcggag
     5821 atgaaggtgc cttaaaattt actaataatg ttttaaatat atgctatata atggctatag
     5881 taatagctat tattggtctt ttatttacag agcaatttgt tacagtattt gcagcaggat
     5941 ttagaaacga ccctgctaag ttccaagcag caatattatt tactaaaata atgatttcag
     6001 gagttttatt cctgagtgga agtaagatat ttagttcgtt tttacaggtg aatgacagtt
     6061 ttgtaattcc tgggcttata ggaatcccat ataatattat aataatagca gcaatagcac
     6121 taagtgcagg taaaaatgta tggatactgc cagcaggagc actattggct atggcaagtc
     6181 agctattgtt tcaactgcca tttgcgttta agaagtccta caagtataag ccatatataa
     6241 atttaaagga tgagtccata aaagagttag tcaacctagt attgcctatg ttggtaggag
     6301 tagctgttgg acagttaaat atatttgttg accgattatt ggcaaccact ttgggtgatg
     6361 gaaagttgtc tgctcttaac tatgcaaata ggctaaatga gtttgttatg gcattatttg
     6421 ttacatctat aataacagtt atatatccta aactagcaaa aatgtctggt aaagataata
     6481 aagaaggatt tataagtact attgtaaaat cttcaaattg tattatattg gtggtgcttc
     6541 ctatatctat aggagctatt atattagcag agcctcttgt tagaatatta ttccaaaggg
     6601 gtaaatttga tgcgttatca acagacctta catctatagc gcttagatta tattcactag
     6661 gtttattggc ttgtggagta agagatgtct tatatagggc attttactct ctttcagata
     6721 caaaaacgcc aatgataaat ggaagtatag ctttaataat aaatatagtt cttaatttaa
     6781 tattgataag accattgggt catgcaggta ttgctatatc aactagtaca tcaaacataa
     6841 ttacagttat attattattt atatctctta aaaagaaaaa tggatatttt ggaggggata
     6901 aaattataaa gactggtcta aaaagtttgg tagcatctgg tgtgatggca gtagctactt
     6961 tattgatata taataatcta tatgcattta tgggttcagg aacaattaaa gaaattatat
     7021 cagttggagc tggagtatta ggtggagctt ctgtatatac tgtattgata gttttattta
     7081 aggtagaaga gatggattta gcttttgaat ttttaagaaa aggaaaacaa aaactactta
     7141 gaagatagat aattaccttg aaattttaaa ttagagtcag aaaggatgaa aatatggatt
     7201 ataagaataa ttatgagatg tggttaaatt ctccttattt tgatgaacaa acgaaaaatg
     7261 agctattaag tataaaagat gatgaaaaag aaatacaaga tagattttat aaaaatttag
     7321 agtttggaac aggtggttta agaggtataa taggagcggg aactaataga atcaatattt
     7381 atactgttag acgtgcgaca ctgggcgttt taaattatat catgaaaact caaggagaag
     7441 aaggtaagca aaagggaata gttatagcac atgatagtag atacatgtct agggagtttt
     7501 gtatagaagt agcaaagact ttaagtgctt atggtgtaaa ggcttatatt tttgaagaat
     7561 taaagcctac accagaactt tcttttgctg ttagatattt aaagtgtgct atgggaatag
     7621 taataacagc aagtcataat cctaaagaat acaatggata taaagtatat gactcagatg
     7681 gtggtcaaat ctgtatagat atggcaaatg atataatagc agaagttaat aagattgatg
     7741 attacagtac tattaaaagt atagattttg aagaagcttt atctaaaaat ttaataacta
     7801 tattagataa tgaagtagat gatgagttta taaaagctgt taagaagcaa gttttaagac
     7861 aaaatataat agatgaatac gggaaaaaat taaaaataat atatacacct attcatggta
     7921 caggaaataa gccagtaaga aaagtcttaa atgaatgtgg ctttgaaaat gtaatggtag
     7981 ttaaggagca agaattgcca gattctaatt tttcaacagt aaagtatcct aatcctgaag
     8041 aaaagtcagt gtttaatata gctattgaaa tggcaaagaa caatggcact gatttgataa
     8101 taggaacaga ccctgattgt gatagagtag gtatcgttgt aaaagattca agtggtgaat
     8161 atgtagtctt aaatggaaat caagtcggtt cacttttagt tagatatata ttagagagtt
     8221 tagttgaaga aaataaactt cctaaaaaca atcctacaat aataaaaact atagttacat
     8281 cagaactagg tgcaaaaata gcaaaggctt ataatgtaga ttgtttaaat actttaacag
     8341 gatttaaatt tataggagaa aaaataaaag cgtttgaaga aagcaatgat agaagtttta
     8401 taatgggata tgaggaaagc tatggttact taattggaac tcatgctaga gataaagatg
     8461 gtgttgtatc ttcacttatg atatgtgaaa tggcagcata ctattcttca aaaggtatga
     8521 acttgtatga agctttgata gatacatata ataaatttgg atactacaaa gaggacttaa
     8581 agtcagtaac tttaaaaggc atagatggaa taaaaaaaat aaaagagatg atgctttact
     8641 ttagaagtgt taaaatagat aatgtggctg atgtaaaagt agataaaatt ttagactata
     8701 aagatggtgt tgatgattta cctaagtcag atgtacttaa atttttactt gaagatggct
     8761 cttggatagc tataagacct tcaggtacag aacctaaaat taaattttac tttggagcaa
     8821 attcagacaa tcaagaagat gtagaattta agttgaacaa tttaatatct tatatattaa
     8881 atgtagtgga ttctatataa aaagctaagg ggagataaat aatgaaagta tacaatgtca
     8941 taatggctgg aggaggagga actcgtttct ggccacttag tagacaagaa gtacctaaac
     9001 agcttataaa tttaagtgga gaagatgctt taataaacga aactataaac agaatagatt
     9061 ctctagcaaa aaaagatgat ttatttatag taaccaatga gaagcaattg gaagctttaa
     9121 aggatatagt aaaagataaa tgtttggata gtaatatatt gccagagcca tgtgccagaa
     9181 atacagcagc agctataggt tttgcagcat ttaacataat gaaaaaatat ggtgatggtg
     9241 ttatgtgtgt atatccagct gaccattata taaaagatga aaaagaattt aaatctattt
     9301 tggaaaaagc tatctatata gcagaaaata atgataaact agtaactatt gggataactc
     9361 ctacatttcc atcaacagga tatggataca taaattttaa tagagaaaat actatagaag
     9421 atgttgcata tgaggtagtg gagtttgtag aaaaaccaaa ctatgaaatt gctaaagaat
     9481 atgtaaactc taaaaagtat gtatggaata gtggaatgtt cgtatggaag gtatctaaaa
     9541 tactagaaga ttttaagaga tacttgccaa aagtatatga aaaattagaa gatataagta
     9601 aatacttagg aacaaaagaa gaaatggaaa aaataaaaga aatataccca actatacaat
     9661 caatctctat agattatggt attatggaaa gaagtaatga tgttatagta gttcctggtg
     9721 attttggatg gaatgatgtt ggttcttggg attctctagg tgcaatatat ccaactgatg
     9781 atgaaggtaa tatcaaaaga ggtgaaaata ttactataga tactaaaaat tcaattatat
     9841 attcagatga caaattaata tcaacaatag gtattagtga tttgatagta gtatctacaa
     9901 atgatgcagt tatggtttgt agaaaagata aagctcaaga tgtcaaaaaa atagtagagc
     9961 agttaaaaga agaagataga caagaatata tgtaggaggt gttcatttga ttcctaaaaa
    10021 aatacattat gtatggtttg gtggtccaaa aggaaatata gaaaatatat gtataaattc
    10081 ttggaaagaa aagcttccag aatatgagat agtagagtgg aatgaaaaaa actttgatat
    10141 agagaaagaa ataaaaggaa ataagttttt ggaagaatgt tataaaagaa aactttgggc
    10201 attcatatct gattatacaa gaataaaagt actctatgaa caaggtggag tttatatgga
    10261 tacagatatg caaatattaa aggatataac tcctctttta gaaaataata ggctaatctg
    10321 tgggtatgaa gatgatagag agtatataaa tggtgctata ataggtgtag aaaaaggaca
    10381 tcctttttta aaagacttat tggagtatta tgaaaaagaa gtacttactt cttcattgtt
    10441 tacaatacca aagataatga ctcatcttat ggaaaagaat tataagaaga ttgacccaaa
    10501 taattatgaa gaaggaatac gtgtttacga caaagaatat ttttatcctt ttggatttaa
    10561 agaagacttt acaccagaat gtataacaga aaacactttt gggatacatt ggtggggaaa
    10621 aagttgggct aaaaagagaa attatttctt agaaagcaaa cacttaacag gtgtaaataa
    10681 gatatggaag tgttgtaaaa tatttgcaag taatacttta cgaagctaaa agggagaaag
    10741 taaaatgaag actttaaatg atgatagaaa tattatgatt agaaaatatc tttatatatt
    10801 tgttttgttt atcatattac ttagaaagta tatgatatca tttattgacc caaatatgga
    10861 tatagggatg attaaatcag cgttatttta ttcttcaata ggaatactaa tgctactttt
    10921 tttatttgac aagagaaaaa gtataacaga aatggtacta gttggagttt gtgtcttact
    10981 ttatttatta aatagggaag gagctatatt attaatcgtt ttattagctg tatcagctaa
    11041 gcaaatagat gataaattta tagttaaaaa ttatttaata atatcagcat gttttttaat
    11101 ggtagctata ttattattta atctatttcc ttcattgata tttaatcagg aagtccacta
    11161 tagatatata gaaaaaatag atatgcttgt tacaagaatg gattttggac ttggtaatcc
    11221 aaatagtgta ttttatcata tggttactat atatgcagca tatatattct taagatataa
    11281 agattataat aaatgggata gaataatttt atttggttct gcattttttg tttatcaaac
    11341 cacatatagt agaactggat ttttcactat attagcagga ttaatatttg tagaaataat
    11401 aagatggata gatataaaaa aaataaaagg actaccaatg ctcctaaaga cattaccgat
    11461 aatacttact ttatttagtg taattattgg tacagtattt gataaaagca ctttattaaa
    11521 tagattatta gcaagtagac ctaagttctg gcatgtatac ttggctgaaa aggggaattt
    11581 tctaaaacct tttggaaatt catatagtcc tgctataaaa gcaactaatc cattagatag
    11641 ttcttatgta tatataatta gtgtactagg agtagttgct tgtatattat ttatgtattt
    11701 gatgtataaa ggaatagaaa gctttataga aaaagataaa aaagcatatt tagtagcttt
    11761 atttatattt ttactatact cttttgcaga aaatttatta ttagaagctt catttagttt
    11821 tgcagtagta ttactaataa aagaagtgat attaaatgat aaaagggaaa tagatttgtg
    11881 gaagatgaaa agtaggaggt agtaatgtta atatcattaa taatgccaac attaaataga
    11941 tatgatgata tatacttact tatggacagc ttagaaaatc aaacttacaa aaactttgaa
    12001 cttatagtag tagaccaaaa tgataatagc aaagttaaag aaatagttga taaatacatt
    12061 gataagttag atataaagta tataaaaagc tctaaaaaag gattgagtta taacagaaat
    12121 gtaggcatag acaatgctgt agggcaaatt ataggtttcc ctgatgatga ttgtgtctac
    12181 gaaaatgata cattagaaaa agttataaat ttctttaata aaaataaaga ttataaaata
    12241 tacagttgca aaactatgga ttcaaataaa gttgatgcat ttaagaaaat gtatgatgga
    12301 acttgtgata tcacaagttc caacgttttg gatactataa catccataac tttttttatt
    12361 gattttgaag gtaaagacta tacaagattt gatgagaaat tgggtgttgg tggagagttt
    12421 ggtgctggtg aagagataga ttatgtttta aacttattga gtttaggttt taaaggaaaa
    12481 tactttggaa atgatataat ttaccatcct gcaaaaaaac attctaaatc taaagaaaag
    12541 tatcaaaaag attataacta tggaagaggc tttggagcgc tttgcaaaaa ggaaatagtc
    12601 tatagaaaaa attataagtt tgcaaaagtt atggtatcaa aacttgtaag aaatatagga
    12661 ggtcttatat tgagttccaa tagagattat catagtgcca ctataaaagg aagaattaat
    12721 ggatttagac agtacaagtt ataatgagga tggtgtttat ggacaaggtt aaatttttaa
    12781 taaatatgct tttggctttt tttatatatc catttaataa gcataagttt aatggtagag
    12841 atatctggct tataggtgga cattcagggg atatatacaa tgataattca aagttttttt
    12901 atgagtatat gctaaaagaa cataatgatg tagaaactta ttgggtagta aataaagaca
    12961 gtaaagtatt tgataaaatt ccaggcaaaa aattaataag aggaagtgta gaaaactatc
    13021 tgtattatta caattcaaaa gctatagtat tttcacatgc accatcagca gatatagcac
    13081 catacaattt tgctgtacct gttcttaatt attttcataa aaaaacgata aaagtgtttt
    13141 taaatcatgg gactataagt tttaaaaaaa gaaaacctat gaacaaaaag ttcaaaaata
    13201 ttatagataa cctatataaa agctataata tagttacagc aagttctgaa tttgaaagaa
    13261 atgttatggt aaatgattgg ggaatgttag atgactcagt ttatataatt ggaaatgcaa
    13321 gatatgacaa tttaccaaca aatgaagttg cacaaactcg agatatatta tatactccta
    13381 catggagaga ttggataaaa tttagttcag gaaaatttac ggatacagat tattttaaaa
    13441 atataatgaa ttttttaaat gatgacaaat taaataaaat attagatgag aaagatataa
    13501 atgtaaaaat ttatatgcat catcttatgc atgagtttat agatgatata aaagaaaata
    13561 taacaggaaa acgaattgtg tttttagata aaggggtaac tctagcaaat gaaatcagaa
    13621 aatcagctgc aaatataact gattattcaa gtgtagccat agactttttg tatatgaata
    13681 gacctatttt gttttaccaa ttcgatttgg atgaatatat ggaaaaagtt gattcatata
    13741 tagatttaaa aagtgaaatg tttgggtctt tggcttataa taatgatgaa gccgtgaata
    13801 aacttattga tattattgaa aataattttg aggttatgga taaccaaaaa aatgaaagaa
    13861 ataagttttt cagatataat gataataaga actgcaagag gatatatgat tgtgtattaa
    13921 gtaaaattaa ataaatattt tataaatgat gaggaaggta atatgaagaa gaatttagta
    13981 tctataatta ctcccatgta taattctgaa aaatttattg aagcaaccat aaaatcagta
    14041 ttaaaccaaa cttatcaaga atgggaaatg ttaattattg atgattgctc aacagataat
    14101 agtcctaata tagtcaaatc ttatatgcaa caggatagta gaataaaatg tataaagact
    14161 gagactaata agggtgtctc taatgctaga aatttagcac taagtaaggc aacaggacaa
    14221 tttatagctt ttttagatag tgatgaccaa tggaatagta gtaagttaga aaaacaagta
    14281 aattttatgt tagaaaatga ctatgtaatt tcatttactt catatgaact gatggatgaa
    14341 aatgataaaa aattaaacaa agtaataaaa gttccaccta atgtagatta tagaagatta
    14401 ttaaaaggaa atatcttagg atgtcttact gttgtgattg ataagtctaa attggatttt
    14461 gaaataagaa tgagtggtgt aagacatgaa gattatgttt tatggttatc gatattgaaa
    14521 aaagggcata ttgctcatgg aataaatgaa gttctagcat tatatagaaa atctagtaat
    14581 tccctaagtg gtaataaaat aaaagctgct atgtggactt ggaatatata tagaaatata
    14641 gaaaaaatac ctttatacaa agctatatac tactttatta attatggaat aaacggaatc
    14701 aaaaaaagtt agacagagag gaagatttaa ttaatgtata aatataaatt tactgtattt
    14761 actccgacgt ataacagagc tcatttactt gaaaatttgt acaatgattt aaaagctcaa
    14821 acttatgatt tcaatgattt tgaatggttg attgttgatg atggaagtag cgacagtact
    14881 aaagagttag ttgaaaaatt tatatcagaa aataaattaa atataagata tatatacaaa
    14941 gaaaatggag gaaaacatac tgcaataaat ctaggtgtaa aaaatgcaga tggagagcta
    15001 tttttcattg tagatagtga tgatggttta attaaagatt ctcttgaaat tgctaatgat
    15061 gagtgggatt ctttagagaa taaagaagga tttagtggag ttgttggact ttgtgtatat
    15121 ccaagtggag atttgatagg gacagaaatg cctgaagata agaaaatatg tcactttgca
    15181 gacctatatt ttaagtatgg cgttaaggga gataaaagca tagtatttgt aactgatgag
    15241 cttataaaat ttccttttcc agaaagaaaa gaagtgagat ttttaccaga aagtgtagta
    15301 tggaatgaaa tgtctaaata ctataaagtt atatgtgtaa ataaacctat gataataaga
    15361 gattatttgg atgatggttt gactaagaat atattaagta aaaatgcatt aagaggtaga
    15421 gcattagagt ttttatattt aataaatcaa aatacatatc cattaagtag atatccatat
    15481 atgtggataa aaaattacat aaatttagcc agatactcat tgttgtctga tagtcattat
    15541 tttggtgaat taagaaaagt atctgataaa ttgttgtatt tagcattatt cccattagga
    15601 tattacaaat atattggaca aagaaaatta gtatctaagt aaacttgttg gtatagtact
    15661 gctagttgct atataaggag agtaaaaata tgaatgagcc tttagtatcg ataattactc
    15721 ctgtttataa ttcagaagag tttttatcag agacgataaa gtcaatccaa aatcaaactt
    15781 ataaaaattg gcagctttta ttagtggatg attgttctaa ggacaattca agtgaaatta
    15841 taaaaagttt tcgaaaagaa gatgctagaa taaaatatat aaagcttgaa aaaaattctg
    15901 gagctgcagt atctagaaat gttggtatta agaatgcgga aggtcgattt attgcatttg
    15961 tagatagtga tgatttatgg gatagtagaa aattagagat tcagatagaa tatatgttaa
    16021 aagaaaatgt aggtttttct tttacatctt atagatatat gagacaagat gggtcaaaaa
    16081 cgaataaggt tgcaagagct cctaagaaaa ttgactatga aggattactt agaaatacta
    16141 taataggatg ttctacagtg gtcattgata aggagattgt aggagagttc agcatgcctt
    16201 tggtaagaag aggacaagat actgcaacct ggttacagtt acttaaaaag gaaaaatatg
    16261 catatgggat tcaagaagat ttagtgaatt acagattagt tggaaattct atatcaagta
    16321 acaagataaa agcattaaaa cgaacttgga atacttatag aaatgttgaa aatttaagtt
    16381 taccaaaaag tctatatgta ttttgtttct atgtttttaa tgccattaaa aaaagagtat
    16441 aagttttata tacttggagg taaaaaaatg aaaatagcta tagctgggac gggatatgta
    16501 ggtttggtta caggtgtttg tctagcagag gtaggcaatg aaaatgtagt ctgtgttgat
    16561 gtagatgaac aaaaagttaa atcaatgaaa cttggaatag cacctatata tgaagaaggc
    16621 ctagaagaac ttatggttaa aaattacaac agaggaagaa tagattacac tacagactat
    16681 aaaaaagctt atgaagatgt agatgtaata ttaatagctg taggtacacc tgagagaagt
    16741 gatggttcag ccaacctaga ttatataaag gcagttgcaa agcaaatagc tgaaactgtg
    16801 acaaaagata ctctagtagt aataaaatct acagttccaa ttggtacaaa tgaagagata
    16861 gaagattata taaatagaaa cctaaaaaat gatgtaaaag tagaacttgc atctaatcca
    16921 gagtttttat cacaaggaac agcagtaaaa gatactctac atgcttcaag aatcgttata
    16981 ggtgtagaaa gtgaatgggc tagagaaata ttagaggaaa tctataagcc ttttaatcaa
    17041 cctataatag ttacaaatag aaatagtgct gagatgataa aatacgcatc aaataacttc
    17101 ttagctctta aaatatcatt tatgaatgat attgcaaatc tttgtgaaat agcaggagct
    17161 aacatagaag atgtagcttt aggaatgagt tatgatgata gaataggaag taagttttta
    17221 aaggcgggaa taggatatgg tggttcttgc tttccaaaag acacaaaagc tcttcactgg
    17281 ttatctgaac ataatggtta tgaattaaaa acagtaaaag ctgcaataga agttaatgaa
    17341 agtcaaaaac ttaaattagt tagaaaggca tctaagttag tagagagctt tcaagatatg
    17401 aaggttgctg ttttaggttt gacttttaag ccagacactg atgatataag agaagcacca
    17461 tctataccaa atataaaata tttgctcgaa agaggggcaa gagttactgt atacgaccca
    17521 attggtgtaa ataatactaa gaaaatattt ggtgactcta tactttatgc tgattctgta
    17581 gatgatgcga tagaagatgc agagatatgt tttataatga cagaatggaa agatgtatta
    17641 aattatgata tacataagta taaggaaaag atgaaaaatc ctttagtcct tgatggaaga
    17701 aattgttata aaattaaaga tatggaagag ttaggaattg agtattattc gataggaagg
    17761 taaatattat gaagactgtt tgttatttag ctgatgggtc aaatcctcat actttaaaat
    17821 ggtgtagttt ttttaaaaat aaaggatatg acattcatgt aatctcttta aatggaggtc
    17881 acatggaagg aataaaaatt tataattttg gttctaatgt agaagaacta aaaaatgaaa
    17941 atatttctaa aaaaacggga tacttaggtt caataaagca gataaagaag ctagtaaatg
    18001 aaataaagcc agatatattg catgcacagt atgcaagtag ttatggattt ataggaagtc
    18061 tacttgggta tcatccatat attatttctg tttggggtac tgatatttat gatttcccta
    18121 ataatggttt tatacaaaaa caaattataa aacataactt tagaaaagca gactatatct
    18181 tttcaaatag tagagatatg gctaaagagg ctaataaata tacagctaaa catgtagatg
    18241 taaccttttt tggagtagat atggataggt ttaagcctat ggaagtagaa aaagaagacg
    18301 cttttgtaat aggaattata aagagtttag aaaaaaaata tggaatagaa tatttaatac
    18361 aagcatttaa gatgcttaaa gatgaatata aagataagaa aataattctt aaaataggtg
    18421 gctctggaag tcagatggac aacctaataa atttagctaa agaattgggt atagaaaatg
    18481 atgttcagtt tttaggaaga atatctccag aaaatgtaag caagactttt aattcttttg
    18541 atgtaactgt atttccttct ttaagagagg gatttggagt ggcagctata gaatcagaag
    18601 catgtgaagt acctgttata gttacaaatg taggaggaca tccagaatct gtatgggaaa
    18661 atgaaactgg cttaattgta gaaccaaaac aaccagaaga aataaaaaat gctataataa
    18721 aactaatgga aaatgatgaa cttagactta atatgggtaa gaaagggcgt caatttgtta
    18781 gagaaaatta tgaggttaat ttaaacttta atgatataga aaaaatttat gattctatat
    18841 ttgataaata taaaaaataa aatgaaaaaa tacagcaaaa gatagatgtt gttggctatg
    18901 cttaggtgcg tagcttttgc tgtttaaagg agggcttcta gttatgcgag aaagtataga
    18961 tttagagaaa gaaagctttt ttaataggct ttttaaaaaa tataaattta gacatttaac
    19021 actattaatt ctagatattt gtagcgtact agtagcgttt cgaatgtctt taagcttgac
    19081 aggtaattta aatgcattta ccactaatga tataataatt tcagcgtgta tatatatagt
    19141 acttcatata gtatcattta gattgtttaa gtgctacaac acattatgga gatatgcagg
    19201 agaagaagaa attatatcta tttttgtggc aactttagct tatttaattc cgatttatat
    19261 aataaataaa ttattagggt ttgattatcc tataatgttt tatgtattaa atacaatatt
    19321 tattataatg tttacaagtg gcgcaagaat agcctataga gctataagaa tagtcatgaa
    19381 caagacatat tctagaggaa aggtttcaaa tatccttata attggtgcag gagatgcagg
    19441 agaaatggtt atacaggaac taaaaagaaa tccagaactt aaaaaagtag cagtggctat
    19501 aattgatgat gataaaaata aaattggaag aataatccac aatgtgaaaa ttgtaggaac
    19561 aacttcagat ataaaagatg tggtagaaaa gtacaatgta gatgagataa tcttttcaat
    19621 agcaaacata gagaaacgta gaaaaaaaga gattattgat atatgtaaaa acactaactg
    19681 taaaataaag acaataccag gaatttacga aattatagat ggaaaagttg atataaaaca
    19741 gattagagaa gtagagatag aagacttgtt gggtagagag cctataaaaa caaatttaag
    19801 agagataagt aattacattg agggaaaagt aatcctaata actggaggag gaggctcaat
    19861 tggttcagag ctttgcagac agattgcagg atttaatcct aaagagctta ttatagttga
    19921 taattatgaa aataatgctt actcaataca acaagaattg cttagaaaat ataaaaataa
    19981 attagattta aaaacagtaa tagcatcaat aagagaagaa aaaagaatgg atgaaatatt
    20041 taataaatat aaaccagaag tggtatttca tgcagcagca cataaacatg ttcctcttat
    20101 ggaatcgagc cctggcgaag ctataaagaa taatatattt ggaactttaa acatagctgg
    20161 cttgtcttca aaatatagag ctaagaaatt tgtccttata tctacagata aggctgtaaa
    20221 ccctactaac ataatgggag ctactaaaag agctgctgaa atgattatac agactatgaa
    20281 tgctgaaagc cagactgaat ttgttgctgt tagatttgga aatgtgcttg gtagtaatgg
    20341 tagtgtaatt ccacttttta agaagcaaat tgaagatggc ggaccagtga cagttactca
    20401 tccagatatt attagatatt ttatgacaat accagaagca gtaggcttag ttatacaagc
    20461 tggggctatg gctaaaggtg gagaaatatt tgtacttgat atgggagaac ctgtaagaat
    20521 attggattta gcaaaaaact taattaaatt cagtggattt gagccagatg ttgatataaa
    20581 aattgaattt tctggtctta gaccaggaga aaagttgtat gaagaactac ttatgtcaga
    20641 agaaggtctg ttggatacag agcataaaaa gatttttatt ggtagaccaa tagatgttga
    20701 tagagaaaaa ataacaaaat acttaaaact tttaagagaa attactaaca atgaagaggt
    20761 tgaaaaaata gatggtatta tgagggaatt agtgccaact tatattaaac cagaagatgc
    20821 taatatcaaa gaaattgcaa caactagaga aaagtagaat atatttctaa tttgtaacca
    20881 gggggagcaa aaagaaacaa atatatacta aaaagaaaca aaagattaca aaaagattac
    20941 aaaaagatta caaaaaaggt tgcaatttat tttgatgtaa gatagaatgt taattaggaa
    21001 ttggaaacga aggaacaaaa tgttaaattt tcgaaaggat taggtatttg agattatgat
    21061 gaatattaaa aataaaaaac atatattgaa aaaatttata gctatggtgt taatagcagg
    21121 agttgtcaca gtagaagcag gagcaataac ggctagtgca gcagagccaa ctaattcacc
    21181 tatgagtgca acagtagacc agtgtgattt tttaaatgta agaagtggag ctagtgcaaa
    21241 tgatgcagta gtaggaaaga taaacacagg agacaaagtt gaagttttag aattacattc
    21301 aaatggatgg attaaaataa aaagtgttga caatgttaca ggatgggtaa atggagatta
    21361 tttaactatt caaggtggaa atgtagatgc aaaggtacaa aatgttttaa acttagcatt
    21421 taagcaacaa ggtaagccat ataaatgggg agcaactgga ccaaattctt ttgattgttc
    21481 tgggttcact tcttatgtct ataaaaatgg agcaggagtt aatttaccaa gagtatcaag
    21541 atcacaagca acagtaggaa agaaagtatc aagagcagaa ttaaaaccag gagatttagt
    21601 tttctttggt tcaggtggtt caataaatca cgttggattg tatgtaggag attctaaatt
    21661 catacattca ccacaaacag gagatgtagt aaaagttact agcatggctc caggaactaa
    21721 ttatgcaaga agacttataa ctgctacacg tgttttacaa taataataga tattgattta
    21781 cataggattg aatattaaca ttaaatatat aattacattt aatgttttac atagagattt
    21841 aagagtttag taatattaaa atagtgatga acgttataaa aacagcaaag aagataaatt
    21901 tctttgctgt tttttttata ataaattatt tcatattata atcaaatatc aaatataaat
    21961 tttaactata ttattcaata tttgacagat aatatgaatt ttagatatac ttaataagaa
    22021 gtatattgta gaaaaaataa atttaaaaaa ttaaggggga tgaaatgaaa aaaataagta
    22081 tattagtatt atcattgata atgacactta ctatgtgtag tgtaagttca tttgctgatt
    22141 catcaaatga taaggaaatg agagctgctt ggatatctac agtttataat ttagactggc
    22201 ctaagactaa gaataatgaa gccaagcaaa aaaaggaata cacagattta ttggacaaat
    22261 taaaaagtgt aggaataaat acagcagtgg ttcaagtaag accaaaatca gatgctttat
    22321 ataaatcaaa tataaaccca tggtctgaat atcttaccgg tacacaaggt aaagaccctg
    22381 gatatgaccc attaccattt ttaatagaag aagcacataa aagaggaatg gaatttcatg
    22441 catggtttaa tccatataga ataactatgg cagatgagag catagataaa ttgccagcaa
    22501 accatccagc aaagaaaaat ccaagctggg tagttaaaca tggaaataag tattactacg
    22561 acccaggttt accagaagtt agaaagtaca tagtggatag tatagcagaa gtagttcaaa
    22621 attacgatat agatggagtt cattttgatg attacttcta tccaggagta agtttcaatg
    22681 atactgcaac ataccaaaaa tatggaaaag gacaaaataa ggatgattgg agaagagaaa
    22741 atgtaaatac tctacttaga gatgtaaaag cgtctataaa gagtataaag cctaatgttg
    22801 tatttggtgt aagcccagca ggtatatgga gaaataagag tagtgaccca acaggttcag
    22861 atacatcagg taatgaaagt tatgtaggta catatgcaga tacaagagca tggataaaac
    22921 aaggattgat tgactatgta gttccacaac tttactggcc aattggtctt aaagcagcag
    22981 attattctaa attagtggca tggtgggcta atgaagtaaa aggaacaaat gtagacttat
    23041 acataggaca aggtatatat aagcaaggtc aatcgagtta tggtggacaa aatatagcaa
    23101 aagaaatagt acaacaagta acattaaata gaaagtatag tgaaataaaa ggtagtatgt
    23161 atttctcagc taaagatata gcaaatagta cttctataca aaaggattta aagagtttat
    23221 attctagtag tgaagaacca gttactccac catctaatgt taaagtggag aaattaagag
    23281 gcgacgaaag atatgacaca gcagtagcaa taagtaaaaa aggttgggct acaaattcag
    23341 atacagtagt attagtaaat gggtactcta tagttgatgg tataacatca acaccacttg
    23401 ctacatcaaa tgatgctcct atactattgg taaataagga taatatacct acatccacta
    23461 agaatgagtt aaaaagactt aatccaagta aagttatttt aattggagga aataactcta
    23521 ttggagataa agttgagagt gagataaaag atacattgtc aaatgtatca ataaatagag
    23581 ttggtggttc agatagatat agtacatctt taatgatagc aaaagaatta gtaaaaacta
    23641 atccagtaga aaaactatat ataacaagtg gtacaggtga agcagattct ctttctatag
    23701 cttcaaaagc aggagaagaa aagcaaccaa tagtattagt atcaaaagat aatgtaagtg
    23761 atgaagttta caattggata tcagatttga aagtaaaaga tgcttatttt ataggtggta
    23821 atctaagtat atctgattca gtaataaata agctggataa ggtcattaca aatgatgtat
    23881 ctaaaaatag aatagctgga gaaaatagag aagaaacaaa tggaaaagta atacaaaaat
    23941 tctatccaaa tgctgaatat tcatcaatgt ttgtaagtaa aagtaatcaa ttggtagatg
    24001 cattaacatc aggtccttta gcagctaaat taaaatcacc agtagttatg cttggaaata
    24061 gtgtaacatc tgctcagaaa actgctttag agcctaagaa aactactcta gtatacgaag
    24121 ctggagatgg tataaatcaa aacacattaa acactttctt aaacttagtt aagtaataaa
    24181 aaagttaatt taaaatacag agtattttat aaatgtagat taaaatgaaa attatcatga
    24241 taaattagaa aataaaaaag aactatatgc attttgtaat caagtgtaca cagcaacttg
    24301 aaaataatta tgcgatatag ttctttttta gtaattaatc ttatactttt taataaactt
    24361 tatgttgaat tttttattgt ttaaactcta tgtcattaaa tataaaatcg tgtaatatgt
    24421 ctaaagtatc tgggtcaaat ctaagaaccc atccagcttt tccatatata ccacctgtac
    24481 tatgaatttc atcatcaata ggaaattcaa attgcttaat atttaagtcg cccattttta
    24541 aaacttgagc tcctaaagaa agtattgcat taggcttcat gttagtttta acatatggaa
    24601 gaactgcatc taataaacct ggatatttag taacaggtaa agtttccgtt ttcttgatta
    24661 tagcttgtat aatctgtctc tgtctgccat ctctagcaaa ggctgtatcg ttgtgtctga
    24721 tacgtgcaaa agatagtgcc tgatatccat ttagagtctg ctttccagag tttctaatgt
    24781 attgtatgct acctttatca tcacttttat tccatttata agtttcagga ataaatttat
    24841 ttagttctcg tatttctcct ttttcaacag taacctcaac gccaccaata gcatcaatta
    24901 tatacataaa tgattcgaaa ttaacacaag cataattttg aatatcaata ttaaaattct
    24961 cttcaattgt ttgtattaat aaatcagctt gtccataagc ataagcatga gtaagttttt
    25021 gtttaccatg accagggatg tctacatagc tatcacgagc taatgatgta agtttgatat
    25081 cattgtgttt gttatcaata gtaagtatca tcatagcatc tgaccttgaa gaatcttcat
    25141 ttggtctagc atctgttccc ataagtagta tgttaattat cccatcttca tttttatggt
    25201 cattgttatc caataaatca ctacttatgc tcgagtcatg aatagcagat aatttataat
    25261 aaaaatatcc atagacactt atagggaata ttactaccaa gaaagccagt agtataacaa
    25321 atttctttaa ttttgacaaa agtacccctt ctttcttctt tttcctgtat attttttaat
    25381 agcatacact taattatatt aaagtattga gatatattca atacttgttt cagttacaaa
    25441 aaaaagacaa tgatataatt tataagaaaa tttatcgacc tatatatata gttaaaagta
    25501 tatataatat attttttaaa agtaatttat attttaaaat aaaaacacac cccataaata
    25561 caaagtaatt tgcatttaag aggtatgtat ttatcatcaa attattggtt tgttttggaa
    25621 tttctaatct tcaaccataa tatctttaaa tatgaaatca tgcattgcat ctagttcata
    25681 ttcctcaaaa ggtattacat agcctgcatt acctattttt ctaccatttt cagtagaaag
    25741 aggaaactcc atagatttca aatttaaatt tcctatactt aaaagttctt ttcctaaaga
    25801 tattatttca ttaggattca tattagtttt tacatacgga agtattgtgt taactagatt
    25861 aggatattta gtgactggta aatccttaac accatttatt aaactctgta taacacttct
    25921 ttgtcttcta tctctttcat ctgtactatc attttttctt atacgagaaa atgcaagtgt
    25981 ctgatatcca tttaattttt gctcaccaga atttcttata tatttaagag aacctttatt
    26041 gtcattttta ttccatttat aagtttcagg aataaattta tttaattctc ttatttcact
    26101 cttatcaaca gtaacagtaa ctccacctaa agcatcaact atatccataa aagagaaaaa
    26161 gtcaactacg gcgtagtttt gtatatcaag ttggaagttt tcttctatag tttctattaa
    26221 taagttagct tgtccatata cgtaggcatg tgttaatttt tgttctccac gtcctggtat
    26281 attaacataa gtatctctat ttaatgaagt tagcttaaga cttttatttt tattatcaat
    26341 agtaagtatc atcatggcat cagaacgaga aacttcttct cctggtcttg catctgtacc
    26401 taacaataat atgtttgtga tattttcttc gtttttatag tcattactac taagcgcctc
    26461 tgtatttata ctatcatctt gtatagtttt taatttagta aaaacatatc catatatact
    26521 aacaggaaat gctataacta aaagggctag aaatataacg aactttttga gtcctgacaa
    26581 taatatcccc tactttctaa attttttaat tacacatagt taattatatt aaaaatttgt
    26641 tacataagca ataaaaaaca caataaatta gcataagttt gtcataatat aattattttt
    26701 atagcatttt atagaagaaa gtgtgtagtt attattgatt ttattggaat ataattgtat
    26761 gaagacttaa atttttataa actgatgtgt atattgaaaa aaatattttc ctgaatgtag
    26821 ttatagcatc ttatgaaagg tgcaaattgt aatttaaaat ttttagtgta taataagaat
    26881 tgaaaattca aaccgataaa aattaataaa aaataattat aaattaataa tttgggggag
    26941 gaaatgtatg ataaaattaa tagcaacaga tttagatgga actttgttag atgaaaagag
    27001 cgaaataaat ccagagtttt ataaagtatt taaaaaatta agagagagag ggataatgtt
    27061 ttctgctgct agtggcaggc agtatcaaaa tcttataaaa aaatttgaag atataaaaga
    27121 tgatatgatg tttatatcag aaaatggaac actagtagta tataaaggaa aagaaatatt
    27181 atcaaatcct ttaaataaag aattagtaaa tgaaatcata gagacaacaa gaagtataaa
    27241 agggaaaaaa attgtaatga gtgggaaaaa atacgcttat atagaaagca aagatgaagc
    27301 gtttatacaa gaggtcagta cttattatgc aaagtttaaa attgtagaag atttgacgaa
    27361 agtagaagga gacatattga agatagctgt atttgacttc aaaggtgcag aacataataa
    27421 taatatctac tttgaaaagt tttctgatag agctcaagta tgtatttctg gagtggagtg
    27481 gcttgattta actgcaaaag gtgcaaataa agggagtgcc ataaaaaaag tacaaaaaat
    27541 gttagatata aaatatgaag aaactatggt atttggtgac cagttaaatg atgtggaaat
    27601 gatgaaaagt gcctatcata gttatgccat ggaaaatgca aatgaacatt tgaaacaaat
    27661 tgctagattt agagcaaaaa gaaatactga aaatggcgtt gtagataaga taaaagaggt
    27721 tataaaaatt ggatagtcat atgtatagat gaaaaagatt attttgaaga aatcaaaaaa
    27781 atatcaatgt aattttgata aataagttta tttatgcgaa aattacattg atatttaata
    27841 aacatcaata atagatattt atttttttat gatattcagt attttgtttt ctatttgagt
    27901 tgcattttca gcagaaatta aataaacata tctacctgcc ttctcaatta tagtattatt
    27961 tgctatttca gaattgattt tttctccatg tccttctcca aaaggtgctt gtatcatatc
    28021 ttttttataa ttttctagag ttttatagat tttatctacc tcgtcagttt ttataacgat
    28081 aatatcttct agagataatt tttcagaaga attaattaca aaaccttgaa ttatgtcttc
    28141 ctctacatca ttaaaatagt taaaatcctt tgcatcaaag atttttgatt cctttataag
    28201 taaatcagaa gattttaatg ctttttttat ttttgatgta gaaacgtttt tgtaatcacc
    28261 acttgaagaa caactaacca ttcctaatga taagattatc gaaaaaaaca atattaaaat
    28321 ttttttcatg ataaacctcc aagtttttta aactctgtct taaattagtt tactacataa
    28381 ttatataaaa ataaatgtat ttaatgttac agttttgatt aataatgtaa tattttgata
    28441 ataataccta ttgagaggtg attagaaatg agaataccaa aacatgtagg aattataccg
    28501 gatggaaata gaaggtgggc aagtcaaaat ggtatgacaa aagataaagg atatgaaagt
    28561 ggaatagacc ctggactaga gtcttataga ttatgtagag atttaggctg tgaagaggtg
    28621 actttttatg gatttacaac tgataataca agacgtccat cagtgcagac aaaagctttt
    28681 acaaaagcat gtattgatgc tgcaaatata ttaatagatg aaggtgctgc tttattagtt
    28741 gtaggaaata caaaatctcc aatgtttcca gaagaattat taccatatac taatagaatt
    28801 aaagccaaag aaggtcaaat aaaagcaaat cttctggtaa attatggttg gtattgggac
    28861 ttaaataata tatcaggagt agaaaatgta aataaaaata atatacagga ttacatacag
    28921 tcatttgatg tatccagact agacttgata atcagatggg gtggaagaag gagattaagt
    28981 ggtttccttc ctgtacaatc aatttatgca gatttttatg ttatagataa ttattggcca
    29041 gattttaaga gtgacgatat aaaggacgca tttagttggt atgatacaca agatgtcact
    29101 ttaggtggat aattaaaaat attctgattt aaggttgtag agtgttttaa attattgaaa
    29161 aaaaaacttc ttgatggtaa aataacacta ataatatgta aaagtgtaaa aatttatacc
    29221 aagaagggtg aagaaatgtc aaaaggcaat aataacaata attcaagaaa caaatctaaa
    29281 aagaccagtc atctgaatag aaaaaagcga aaattaaata agaaaaagtt agcagtgttg
    29341 atttgcttta cagttttatt tttatttatt gcatttaagg ctactcaagg tgtagtagct
    29401 ttggtaaaaa gtatggataa aagtaataaa actaatcaac aacaaaatgt taactcagaa
    29461 caatttgacc ttggtaatga agaggaaaat aaaaagaaaa aatatactgt atttatagac
    29521 ccaggtcatg gaggcaatga taaaggaact gaaagcaaga ccagtaatag atatgaaaaa
    29581 gacctaaatc tacaaattgc aaaaaaatta gcaaataaat tatctaaaca aaaggatatt
    29641 caagtagtag tatccagaac tgatgataca tatatatcat taaaggatag agctatctta
    29701 gcaaataata gttctgctga tgtgcttgtt tctattcatt taaatgcaga gaaaaatggt
    29761 aatacagcaa caggtataga aacttggtat aggaataaag ctacagatgg ttcaaaagaa
    29821 cttgcacaga cagtacaaag tactatagta tcctatgtta aagttagaga taggggaata
    29881 gttgaaaata actttgaagt acttagagaa tcaaatatgc ctgctatatt aatagagtgt
    29941 ggttttttaa caacaccttc agaggaacaa aaaataataa atgaaaaata tcaagaccaa
    30001 cttgcggaag gaattgttca aggtgtactt tcatacctag attctaaagg gaataagtaa
    30061 gttaacttta ctttaatgtg aggataaatt taaaagttta actgtgtaaa ttgtattaac
    30121 tttatttcgt aatgttgcaa acattgtttg aatttagaaa ctatgaaatt agaaattatt
    30181 ttatctatat ctaatttcat agtttttttt gcaaaaaatt tatagttata aaattttaaa
    30241 aaataaattt agtataatgt aatgtagtta tttatatgtg attttaactt gcattgattt
    30301 gctatcaatt gtgctttata attagtatat ataaagttta actgtaagat aatttaaaaa
    30361 ataggggaat ttatatgaat aaaaatagca tgacaagaat ttacagttta aataaaataa
    30421 tttttattat aattggaatt ttaggaatag ttttactcac aagacatatt gaggtagata
    30481 ttaaagtttt aattttaatt ttattaacct tgtttagtat aaatgtattt atttcatatc
    30541 ttaaaattaa gttgtatgag gataaaatta atttaggatt gataaataat tcagataagt
    30601 ttataaatat tactaaagat gagaacttcc taaaacatgt tcaaaattat attatcagta
    30661 atgaaaaaga aaactgtgta atggcttgtt ttgatttatg tagactcaaa cttataaatg
    30721 atatatatgg ttatgagcta ggggatgaag tgttaaataa tattttaagt aacttaaaaa
    30781 attattttgg aaaagaagct atttatggaa agttaaaaag tgatgttttt gtattaataa
    30841 taaagcttga aaatagggaa cagaagattc cttatttagt aaatcttatt aagaataaaa
    30901 ttgtgatact aagtcagatt gacagtttta aaatggatat aaatattgag gcttctattg
    30961 gaatttataa atttaacaat aatgaaattg atataaaaaa agctatagat aatgctgata
    31021 tggcaagact taaatcaaaa ggactaaagc acatagagta tgtagaattt gataatgcta
    31081 tggaagaaga aatacaatca attatgaaaa tagagagaga tttattcttg gctattaaaa
    31141 ataaagagtt tgtaattcac tatcaaccta aagttgattc aagtacaggt aacattatag
    31201 gttcagaggc attgataaga tggttgcacc catcattagg catggttgga ccaaataagt
    31261 ttatacctat agctgaaaaa aatggtctga taaacaatat tggtagatgg ctaattcaag
    31321 aagtttttat taccattaac aagtggattt cagaaggtat aaatgtctta ccagtctcaa
    31381 taaatctttc tagagtagaa ctatataaaa atgatttagt tgattttttt aaactgatgt
    31441 ttaatacata taatattcca aaagaactta tagaattaga aattacagaa acgacagcac
    31501 taagagatgt aaaatttata agtgaaaggc tttatgagat taaatcttta ggtatgaata
    31561 tatcattaga tgattttgga gtaggtaatt caaattttat caaccttaag gggataccac
    31621 tagatattat aaaaattgat aggtcactta tattggatat agttacaaat acaaaaacaa
    31681 agtttatggt gaaagcaatt gttaaccttt cacatgattt aaacgtaact gttatatgtg
    31741 aaggggtaga ggatatgcat caggtcaaag tcttaagtga actgggatgt aatttcatac
    31801 aaggttatgt attttttaaa cctttggatg agatgaatta taaaaaactt cttactgacg
    31861 gaagttatat aaatctgaaa gatactcttt tagataaatg catgacagtt gaggaggagt
    31921 aaggatgatt tcagaagatg tagattattc aataagaatc ataagagcta tatttaataa
    31981 tggagaagat aaaccaatag atgctaaaat gatattaaaa agtgaagata taccaatact
    32041 aacactatac agactgctta gaaagctttg tgcaaacaat attcttactt ttaacaaaga
    32101 agatggcaag acatacatgt taaatgtgtc attggataca ataagtctaa gaactattgt
    32161 agaagtaatt gatggaaaaa tatatataaa taaatgtacg agatatgtat ctaattgcag
    32221 taattgctta aaaggttgta aagtaaatcg tgaattgcta gaaatagaaa aagaaattta
    32281 taaactttta tcgagaaaat ctataagaca aatactagaa taaaaaattt tcagctatga
    32341 taataattgg aggttttcgg ttatggatga tatatctcaa gataattttc tactgtctaa
    32401 agagtatgag aatagcttag atgtagatac taaaaaagca agtggaatat attatactcc
    32461 taaaataatt gtagattata ttgtaaaaaa aactttaaaa aatcacgata taattaaaaa
    32521 tccatatcca cgaatacttg atatttcctg tgggtgtgga aattttcttt tagaagtata
    32581 tgatatttta tatgacttat ttgaggaaaa tatatatgaa ttgaaaaaga agtatgatga
    32641 aaattattgg actgttgata acatacaccg tcatatatta aactattgta tatatggtgc
    32701 tgatattgat gaaaaagcta taagtatatt aaaagatagc ttaacaaata aaaaggtagt
    32761 aaatgattta gatgaatctg atataaagat taatctattt tgttgtgata gtcttaaaaa
    32821 gaaatggcga tataagtttg attacatagt gggtaatcca ccctatattg gtcataaaaa
    32881 acttgaaaag aaatataaaa aatttctcct agaaaaatat agtgaagttt ataaggataa
    32941 agctgactta tatttttgtt tttataaaaa gattatagat attttaaagc aaggagggat
    33001 tggcagtgtt ataactccaa gatatttttt agaaagtcta tcaggtaaag atttaagaga
    33061 atatattaaa tcaaatgtca atgttcaaga aatagtggat tttttaggtg caaatatatt
    33121 taaaaatata ggggtatcta gttgtattct aacttttgat aagaaaaaaa caaaagagac
    33181 atatatagat gtatttaaaa tcaaaaatga ggatatatgt ataaataaat ttgaaacatt
    33241 ggaagaactt ctaaagagta gcaaatttga acattttaat ataaatcaga gattgctatc
    33301 tgacgaatgg atacttgtaa ataaagatga tgaaactttt tataataaga tacaagaaaa
    33361 atgtaaatat agcttggaag atatagcaat aagttttcaa ggaataatta cagggtgtga
    33421 taaagcgttt attttaagta aagatgatgt aaagttgaac cttgtagatg ataaattttt
    33481 aaaatgttgg ataaaaagta agaatataaa taaatatata gtagataaaa gtgaatatag
    33541 gctaatatac tcaaatgata tagataatga gaatacaaat aaaaggatac tagatgaaat
    33601 tatagggcta tataaaacaa aattagaaaa cagaagagaa tgtaaaagtg gaataaggaa
    33661 atggtatgaa cttcaatggg gtagagaaaa gcttttcttt gaaagaaaaa agataatgta
    33721 tccttataaa tcaaatgaaa atagatttgc tatagattat gataataatt tttcaagtgc
    33781 agatgtatat tcttttttta taaaagaaga gtatttagat aaattttcat atgaatatct
    33841 agttggaatt ttaaattcca gtgtttatga taaatacttt aaaataactg ctaaaaaaat
    33901 gagtaaaaat atatatgatt actatcctaa taaggttatg aagataagga tttttagaga
    33961 taataattat gaagagatag agaatttatc aaagcaaata atatcaattt tattaaataa
    34021 gtctatagat aaaggaaaag tagaaaaatt gcagataaaa atggataact tgataatgga
    34081 ttctttaggt atataagcag ttgtaagtat taaaaaatta taatctttta attgtttatt
    34141 taaatttaga aataaaaagt tagccatata aaaaatatta aaaatatata cttttcaata
    34201 tttttttgat aaaataagaa tgaatttgtg caattttatt tttctccaat aactaaaaat
    34261 agttttttga attaatattt gcttaagaat aaaaatcaga aacgtttttg atatagtagg
    34321 tgataatatg aaaggattag ttttagaagg tggaggaaca aaaggagcct accaaatagg
    34381 tgcatataaa gctttaagag agcttggtat agagtttcaa ggagtaactg gcacatcaat
    34441 tggtgcttta aatggagcat atatagttca aaatgatatt gaagtaatgg aagatatttg
    34501 gttaaattat gactatactc attttatgaa tattgataga gaaacctatg agaggtttaa
    34561 aaatgtagat ttcactacta aaaatctcaa tgcagtgttt gagcttataa ataaagctag
    34621 aaaaaatgaa ggtatagata taagcccatt aagacaacta atggagacaa cattaaatga
    34681 agacataata aggaaatcaa aaaaagactt tggacttgtt acggtctggt gggataaaaa
    34741 aattaatcca tatccattgt atatcgaaga tatacctaaa ggaagattga ttgattattt
    34801 aattgctagt tccagtttgc ctatttttca aacgaacatg atggatgata agttgtatct
    34861 tgatggaatg ttttgtgata acatgccaat aggactcttg gaagacaaag gttatgaaga
    34921 tgttgttgta attaggttga ttgatgattt tattgggaaa atgaatctaa atcgacatca
    34981 aaatataaat ataaaaacta taataccatc agaatatcta ggaggttctc ttaataaaga
    35041 tagagataat gtagaaaaaa acatccgact aggttactta gatactatga aagcatatga
    35101 aagatgcact ggaattaagt attattttga tgtagagtat aaatataatg aagaatattg
    35161 ttttgataaa tttagaagta tgagcaatga aacaatacaa tatatctgta atttattgaa
    35221 tataaaaaga gatgcttcac ttagaaccgt tatggaaagt gtaataccaa agcttggtga
    35281 ggtgcttgga ttgccaaagg atttttcata taaagatttg ttttactgta tatatgaaaa
    35341 gaaacttgaa gaaaataata taaacaggat aaaattatat gattttaaca aggttataga
    35401 tgttgtaaat acaaatattg attctaatag aaatttagct tctaattttg aagttagacc
    35461 taaaattagt attaaaaaga acaaaaacac aaaattattg acaaattgta ttataaaaga
    35521 cataaaaaat caaagttaaa taaaaaacaa aaaaaaattt gtaattatac tcgaaaaaag
    35581 tgtatattag tagtataata tttttgtagc actagtggga acaaaaaatg accataggac
    35641 aaaaaagtac cacttagtac cattaaagta aaaaacagga aaacaatttt aaaaaatata
    35701 aaatgtaaag tttataggag gatttaaaaa aatggaaaaa gtagttagta tcaaaaatgc
    35761 aagtggatta catgctagac cagcaggaat gtttgttaag aaggcagctg aatttaaatc
    35821 aacagtagag gttgtagcaa aagacaaaac tgtaaatgca aaatcaataa tgggaataat
    35881 gagtttaggt ttagctcaag gagaagaatt aaagttagtt gtaaatggag aagatgaaga
    35941 agtagcttta gctgcaatgg ttgaattagt tgaaagtgga tttggagaat agatataata
    36001 attttttaaa aatttgatat ttagaatcta atattactta aagtaaagta acagagaagt
    36061 attaaacata tacagaccaa catttgtaga tatttaattt ctttgttact ttcatgttta
    36121 tttaatcaga agattaagac tattgttaat tgggtataaa aatataaata ttttagaaaa
    36181 tgaaataaga tatttttaat cataaattca gagtaaaaat acagtaacaa atagtttata
    36241 aaattttata aaaaattata agtaaataaa actataaaaa tgtaaattat ttatataaat
    36301 ttataaagtt tatgtgctag atgatatttt ttaggaggta gggttatggc ttacaaagga
    36361 ataggtgctt ctcctggtgt tgctttaggg aaagcgttag tagttgaaca tagtgaatta
    36421 gtaattgaaa aaaaatctat agataatgtt gaagctgaaa ttgctaaatt agaagatgca
    36481 gttgcagttt ctaaagaaga attagtaaaa gttaaggaaa aggcttctga agaattagga
    36541 gcagaggaag cagaaatatt tgaagcacat ttattagttt tagaagaccc agagttaatt
    36601 ggttctgcaa tagataaaat taaaactgaa agtgtaaatg cagagtatgc tttaaatgaa
    36661 ataaaagaga tgtttgttag catgtttgag tctatggaca atgagtacat gaaagaaaga
    36721 gcggcagata taaaagatgt tacaaataga atacttagac atatattagg aattaaagtt
    36781 gttgatttat ctgctttaag cgaagaagtt gtattaatag cacatgattt aactccatca
    36841 gacacagcaa ctatgaataa aaaaatggtt ttaggatttt taactgatat aggtggaaga
    36901 acttctcata ctgctatcat gtctagaact ttagaaatag ctgcaatcgt tggattaaat
    36961 gatataactt caaaagttaa ggatggagat tttgtggttt tcaatggaga cacaggtgaa
    37021 gtaatcgtga atcctgatga agagactata aataaatata ctgaattaaa agctaagtat
    37081 gaagatgaga gaaaagcatt acagctatta aaaggaaaac catctgtaac tttagacgga
    37141 aaacatgtgg aattagctgg taacatagga acacctaacg atatagaagg tctaataaag
    37201 aatgatgctg aaggtgttgg tctttataga acagaatttt tatacatgga tagagattct
    37261 ttcccaacag aagaaataca atatgaagca tataaagctg tattagaagg aatggatgga
    37321 aagcctatag ttataagaac attggatata ggtggagata aagaattaag ttatttaagt
    37381 atggaacctg aaatgaatcc attcttaggt tacagagcaa taagactttg cctagataga
    37441 aaagatatat ttaagactca attgagagct ttatatagag ctagtgttca tggaagatta
    37501 agaatcatgt tcccaatgat ttcatcttta gaagagttgt tacaagctaa agaagttgta
    37561 aaagaagttt tagcagaact tgattctgaa ggtgtggctt atgctaaaga tgtagaaatt
    37621 ggtatgatga tagaagttcc atctgctgct gtgatatctg atgtattggc taaacacgta
    37681 gatttcttct ctataggaac taatgattta attcaataca cttgtgcagt tgatagaatg
    37741 aatcaaaaga taagctatct ttacaatcaa tttaatccag ctgttttaag acttataaag
    37801 actgttatag acaatgcaca taaagaaggc aaatgggcag gtatgtgtgg agaatctgct
    37861 ggagaccaaa aaatgatacc aatactttta ggtatgggat tagacgagtt ctctatgtct
    37921 ccaatatcaa tacttcctgc tagaaagtta ataacttcag ttaaagagtc agatatgaaa
    37981 aaattagctg atgatgtact taatatgggt actgctgaag aaataaaaag ctatatagaa
    38041 aagactttta atatataatt aatgttatat agttactaaa attcattttt ctaaataaca
    38101 aaattatatt ttacgaatta aaaaggagat gtttcaaaat aaaaatttga agcatctctt
    38161 ttttataaaa gattactaag aattacttaa tctttgtaat agtcattttg tgaaaaagca
    38221 aaactgcatt gtggctatag tatatatgga tgttattatc aataaaagta ctattaaaat
    38281 gaagattcat tctcaaatga tatcaaaagt agttgtgatg gagtaagata atattttgtg
    38341 atttaatagt aaaaattaaa aaaatatggt attgaaaaat aattaaaagt atttaataat
    38401 agaattaggg aagaataagt ttataagtaa aaataattga ttactagtta ataaaataaa
    38461 tgtgatatcc aaataaatcc taaatctaat ttgagtttta atatagatag tctaaagttt
    38521 gattattaaa gatttaattg taagttgtta cagttgatga tattgattag agattaaagt
    38581 ttagagtaga aagttacctt aaatttaaaa atgcaagata acccaatcta aaaattataa
    38641 tcactcaata ggtttaagga tttatttgtt tattataaat aggagggtgt ctcaaaatga
    38701 actttttagt ttatgaggca ctctctttta tatagaatta aatatatttt attatttcaa
    38761 caatgaaaaa gaagcttatc tctattttga gacagcctct ttgtatacta ttattaattt
    38821 aatcaataac ataatttaaa actatattat tagttaagat ttcctgcttt attagtaaat
    38881 aattctttaa tagcattgtt tctggaaact aaatcgttat cagagttgtt tgtttttttc
    38941 ataatcactg aataacgttt tttatttata aaaaatatta aaatcttatc atcatcaaaa
    39001 ttgtattctt tatatctgtc aatagactca taacctagat ttagaatgtt tttgtctttt
    39061 atactatcta catctaaaat atatgatggg tcagccttta gtaatatatt aattaaacat
    39121 ttttcttcac cagcacagaa taactccatc ttacttgata tagatttaga ttgctctgaa
    39181 gttatttttg caggaataag gttgtcattg tcatctctta gaagataacc atgtgtgtct
    39241 tgttgatata ctaaattaac ctttttctta ggaacactaa tcattttatc taaatgtaca
    39301 taatgatgat tttcaacaaa accttcattt aatatttcaa caaagttatt aattttacct
    39361 aggcaaaaag attctataga ttctttacag aatttatttg taaatggtat tatgtttacg
    39421 ttttcctcgt ctgtattcca tgctatagca taatccaacg aatatacttc ttcctctata
    39481 tctgcaaaat tatattgtac tatgtttcct ttcaaaatta ctcgacctcc atcttataat
    39541 atatttaata tactttataa aacttatatt atatttaaat aatatcataa attgaataaa
    39601 tatgatatag taaataagaa aaaatttgaa aaaaatcact ataaatgagt aataaatata
    39661 aatagtgtat actaatggga tggaaaatgt ccactattaa ctttattacc ccacatatat
    39721 aagataaaat tattaatata aaatataatc tatcgacaat gaatagactt ttaaataaac
    39781 ttaatataaa attaattaaa taagtgctta aaaacgattt actttaatga tagaatcata
    39841 ttaaaggagg ataattttat ggatataaag aataaaatta aatctatatt tttaccagaa
    39901 aaaaatgact atttagatga tgaagaattt gagtatgata tagaaaataa tgaagaagat
    39961 ttaaaggaag ataacaaagt acatttaaat gatttaagca gagtaaaata tgattacaga
    40021 gagttaaaag acatagaaaa ccaaacagaa gagatgtgtc tgctagcagt aaagcaggat
    40081 ggtactataa ttgagtttgt aaaggacaag acttataaag tatgtatgga agcagtaaaa
    40141 cagacatata aatctcttaa gtatataaca aatcaaaatg aggatatttg tatagaagca
    40201 gtaaaacaga actacagagc tttgtactac ataaataata agacagaaaa tgtactaatt
    40261 gaggctataa aaaatgcatc aacttatgat gtcatggaag tatttaaatt tgtggaagag
    40321 caaacagaag atgtatgtct agcatttata gaaagggcat ctaaaaatga tgttgcagag
    40381 atattaaaag gaataaaaga gcaaactcca gccatatgtt tagaagctgt aaaaaaagat
    40441 ggaaaatctt tagcttatgt aaaggaacaa agtaatagta tatgtttaga agctgtaaaa
    40501 gaaaactaca gtgcactatc ttgtgtaaaa gagcaaactg aagaaatatg tatagaagca
    40561 gtaaaacaga atgactttgc attgtattat gtaaatgaac agacagaaaa aatatgcatg
    40621 gaagcagtaa aaagaagtta tatggctcta cagtatgtaa ataagcaaac agaagagatt
    40681 tgtttggaag cagtaagaat tgatggaaga gcattacagt acgtaaaaga gcaaacagaa
    40741 gaaatttgct tggagtcagt tagacaaaat ggaaaagtac tgcaatatgt aaaaaaacag
    40801 actgaaaata tatgtataga agcagtaaga ggttcatttg aagaacttga aataaaagaa
    40861 atattaagtt atgtaaagat accaacagaa agaatatttg tagaagcagt aaagcaaaat
    40921 ggaaaaattc ttaaatatgt agaaaatcag acagaattaa tatgtttaga ggcagtaaga
    40981 gaaaactata atgctttagc ttatgtaaag gaacaaacag aaaagatttg tttagaagca
    41041 gttaatcaaa gttatgaagc gcttaaatat gtgaaagaac aaacagaaga agtttgtcta
    41101 aaagcagtaa aacaagatta tagaatgttg aaatatgtaa ataatcaaac agaaaaaata
    41161 tgtttagaag cagtaaagca aaattataga gctttagaat ttgttgataa tcaaacagaa
    41221 aaagtttgtc ttgaagcagt aaaacagaat agaaaagcac tacagtatgt aaaacaaaaa
    41281 caaagttaaa aggctaaaat atatggggaa tatatgcaaa taaataaaat ttgatatttt
    41341 gtaataaata aaatgggtat ctcaaaagga actttttagt ttgtgaggta ctctttttat
    41401 atgaaatgaa atatattttt agccttttaa caaaggggaa aaggtttgtt tctattttga
    41461 cataggcttt ttatgcatat aaagcttgat aaaattgttt tattataatt gttttgttat
    41521 ataaataata agaagaaaat tatataggtc tttttttgac cagttttaca tttttaacaa
    41581 ctagttttgt aaagtatatt gaattttctc aaaaaagaat gtactctata tatgtaaaat
    41641 ttttttggag gtaaaaatgt ttaaaagata tgctgaaaaa atgacatctg ttttaatttg
    41701 taataatatg atagataata atgaatctaa agtctattct tatggatttg aaatattaat
    41761 agctttcata gttaatataa ccaccatgct gtttataggt tttttatttg gtaaatttac
    41821 atatgtatta ttttttttaa tgtgttattg tccaataaga caattttcag gagggtatca
    41881 tgcagacaat tattttagat gcttattaac ttttattttt ataattttat ccacaatctt
    41941 aattatagaa aatataaata tagacttatt taaaaatatt attatgatta ttgcaagtgt
    42001 tagttgggtt ggaatttgtg ttctgtgccc aatagaacat aggagtaacc ctataagtga
    42061 tagggaaaag ttggtttata agaaaactgc tatatttata tctacagtag tgttattgat
    42121 aacaatttca agtctttctt tgagtatatt tgtagattat ttcacatata gcgcatttgc
    42181 catgttttgg atttttgtga tgttagtatt aggtaagtta aaagctaaag tataagtaaa
    42241 atatgtatga gggagagtta agttatgaaa aagtttattg taagatttat gaagtttgct
    42301 agctcattgg cacttagcac tgctattttg tcagctaata gtacctgtcc ttggattatt
    42361 catcagccta aagtacccaa agaaatcagc aatctaaaga agacaaatta aatacaagca
    42421 cttatttata caaaattatc aggaaaaaag aaattattat attttaataa gattaaaaca
    42481 atataaaatc agatacaaaa tttattttat aggagcctaa tacctatttg gtaatatatt
    42541 agtgcgccaa tttaggggga tatagtgtat ttaatgaaat gacttacgga gagtaagtca
    42601 tttttgtatt gtatgtattt tatgtaaact tatgatataa tgaagggtaa agtttaatta
    42661 aaataataaa aaactttagt atagatagta atttaggagg tagggtatga gtatgataca
    42721 taaattttct atgaatggat ataatatcgt tttagatgtg aatggtggag ctgttcatgt
    42781 gttagatgat gttgcatacg atttgttaga tttttataaa gaaaaaagta aagaagaaat
    42841 attagagata ttaaaatcaa aatatcaaga agaaaaaata aatgaagcat atgaagaaat
    42901 attaaatttg gaaaaagagg gtcttttata tacagaagat acatatcaat atcatccaag
    42961 ctttgtacat agagagcctg tagtaaaagc tttatgttta aatgtagctc atgattgtaa
    43021 cttaaaatgt aaatactgtt ttgcagctca aggtgatttt ggaggagaaa aagaacttat
    43081 gagttttgaa gttggtaagg cagcaattga ctatttgatt gctaattcag gaagtagaaa
    43141 gaacctagag atagatttct ttggtggaga gcctttgatg aactttgaag ttgtaaaaca
    43201 actggttgac tatggaagaa gtgtagaaaa agattataat aaaaatataa gatttacaat
    43261 aactacaaat ggtgtactgc taaatgatga gattatagat tatataaatg aaaatatgca
    43321 taatgtagtt ttaagtttag atggaagaaa agaagttaat gataatatga gaccaacttt
    43381 aaatgataaa ggaagttatg atattacatt accacgattt aagaaacttg tggaaaaaag
    43441 agcaaaagat aagtattatt atataagagg tacttttaca agagataatt tagatttttc
    43501 aaaagatgtt atgcattttg cagatttagg atttaaatta acctctgtag agccagtcgt
    43561 tggagatgag agtaatccat atgcacttag agaagaagat ttacctaaga tatttgaaga
    43621 gtacgaaaaa tttgctgtag agtatgcaga tagacaatta caaggagatg gatttaaatt
    43681 tttccatttt atgatagacc taaatcaagg tccatgtgta ataaagagaa taactggatg
    43741 tggagctgga aatgagtatc tttctgtaac tccaaatgga gatatctatc catgtcatca
    43801 atttgttgga aatgaagaat ttaaaatggc aaatatattt gatgaagaaa tagtacttcc
    43861 agaaaattta aaaaatatgt ttagagaagc tcatgtatat actaaagaag agtgtaaaca
    43921 atgttggaat aagttttact gttcaggtgg atgtcatgca aatgcgataa actttaataa
    43981 tgacatatca aaaccatatg aattaggatg tgaaatgcaa agaaaacgta cagagtgctc
    44041 tataatgata caagctaagt taatgttaga aggagcaacc aactaaattt atattttaaa
    44101 ggagttgttt atatatgatt agagattatt tagaagataa acctttaatt gacgaaagtg
    44161 tatttgtggc aaaatcagca gatgtaattg gaaatgttaa aataggcaaa gattcaagta
    44221 tatggtacaa tgcagtagtt aggggtgatg aaggaccaat aactattgga gaaaacacta
    44281 atatacaaga ttgttctata gtgcatggag atacagaaac tataattggt aacaatgtaa
    44341 cagttgggca taggtctata gttcatggtt gtaaaattag tgataatgtg ttaattggta
    44401 tgggttcaat aatattggat aatgctgaaa taggagaata tactctgata ggagcaggta
    44461 cactaattac atctaataag aagtttccgc caggtgtttt gataatgggt tctcctggta
    44521 aggttgttag agagcttaca gaagaagata aaaaatatat tgatgaatct tatgaatggt
    44581 atttagaggc agcacaaaat caaaaatatt agtttaaaga gtggttgttt caaaacatat
    44641 ttaaatctgt tttgaggcaa ccactaattt attttattag attttgttga tattttagtt
    44701 ttcaaaaagt atttgattaa accagatata aaatgtcaaa aataaaaatt aactgagtta
    44761 aataatttta ataaaattgg tagtatatgt gataaaatta aatattaaag aggatatagg
    44821 atattagaat atcaattggt gaaataatgt catatacgat aaattaaggt gtaagttttt
    44881 gggtatacct atcttaggag tgaaaagatt tgagaataag ttatagaaat ttaaagcgat
    44941 atagagaaat tggatatgtt ttaataaagt atggttttag ttttattgta gaaagactaa
    45001 atatagaagg aattgcctat aaaataccac tatttgaccc accagaagaa ataaaaaata
    45061 tgacaactgg tgagagaatg aaaagagttt tagaagaact aggacctaca tatataaaaa
    45121 taggacagat attgagtaca agaaaagact tgttagacca ggatataatt gatgaaatat
    45181 caaaactcag agatgatgta gagaaatttg attcaaatat agctattgat atatttaaag
    45241 aagaagtggg tttaagtata gaagaaatat tcttagaatt taaagaagaa gcaatagcag
    45301 cagcttcaat tggtcaagtt tatgaaggtg tacttaaaac gggagaagaa gtaatcgtaa
    45361 aaattcaaag accaaatata gagaaaataa taaaatctga tttagagatt cttagaacta
    45421 ttgcaaatac tttaaaagat ttaaaaaagg attttaattt agatttagta cagatgattg
    45481 aagaatttca aactcaatta atgagggaac ttgattatac atttgaagct ataaatgcaa
    45541 ctaaattctc taggatattt aaaaatagtg atgaagtata tatacctaaa gtatattcag
    45601 aatataatac taagaaaatt ttagtaatgg agaaagttaa tggtacaaag ttaagtgatg
    45661 tagaaaaaat aaggagacta ggttataaca caaaaactat tgttgaaata ggcgttaggt
    45721 cattttttac acaggtatta tctcatggat tttttcatgc agacccacat ccaggtaata
    45781 tattcgtagt tgctaaaaat aagatagcat atatagattt tggaatgatt ggaattattg
    45841 ataataaaac tttaaaccaa ttaaatgaaa ttgcattagc aggtgtagaa aagaatgttg
    45901 acaagataat atatctattg atagaaatgg atgcattaaa tggagaggct gatattaagg
    45961 gacttagaca ggatttactt tatctaatac attattacta tgatatttct atagaaaaaa
    46021 taaatgtaac agatatatta aatgaactat ttaggttttt tagacagtat aaaatagtca
    46081 tgcctgcaca atttgtaact cttgcaaaga cagttataac attagaagga acaagtagaa
    46141 ctttaaacac agatttttct tttggttcta tgggaaaaga gtttatgaaa catcattata
    46201 agagtaaatt taaccctaaa aatgttgttt taagttcaag acaaaatgta gaagaaatat
    46261 tactggacat aaaaactatt ccaaagcaat taaaagcaat tttaaaaaat atagaaagaa
    46321 ataatataaa aatgcaaatt gaagatgtta aaatgacgag gttagaaaat tgtataatag
    46381 agctaacatc tcaaatatct ttgagtttag tgttggcatc tattatagta ggttcttctc
    46441 ttataattgc atctccaaat atagaaaata atatatggat aaaatttact gcaatagcgg
    46501 gtttctttat atcctttata ataggccttt gcttagttat aagaagtatt aggtcaaaat
    46561 acaaaaaaga ttagggggaa ttaaatttga aaaaatggat attgaagtat aaaggtaaga
    46621 tagaggaaag tgaatttagc aaaaaactta atatttcccc ggaaatctgc caaattttaa
    46681 aaaatagagg gatatgtact gaaaaagatt cagagatatt tatgaatcca tctttagatt
    46741 atttaagaga ccctttttta atgaaagata tgcaaaaagc tgttgataga ataaagttag
    46801 cagttgagaa gaacgaaaaa atttggattt acggagatta tgatgttgat ggtgtatcat
    46861 ctacatctat cttgtgctta tattttaaga gtataggtta caatgttgat tactatattc
    46921 ctaatagatt agaagaaggg tatggtataa atgaagatgc tattaagctt ataagctcta
    46981 ggggatgtga cttgattata agtgttgact gtggaattac atcagtgtct gaggtcaata
    47041 cagcgaatga tttgggcata gatgttataa ttacagacca tcatgaatgt caaagtgaaa
    47101 ttccatcagc ctttgcagta ataaatccaa aacaagaaga ttgtaattat ccatttgatt
    47161 cactatgtgg atgtggtgta gcatttaaaa tgattcaagc cttgactcca aaagaagaat
    47221 ttaaaactag tatgtataat tatttggaaa tagtaacact agcaactatt tgtgatattg
    47281 ttccacttat agatgaaaat agaataattg taaaaaatgg tttaaaatca atgaaagaag
    47341 gcaaaaatat tggattaagg gagcttataa aagtttgtgg tgtagaaagt gataagatag
    47401 gttcttctca tataggtttt gcaataggtc ctaggataaa tgcttcaggt cgattgggat
    47461 attcttactt aggagtagag ctatttacta ctcaatcaca agaagaggca gtagaaatag
    47521 ctagtatatt ggaagaaaaa aataatgaaa gacagatgat agaagctaag atgtatcatg
    47581 aagctgagga aatgctaaag tctaatagca gatacaatga tgacaaggtg ttggttttag
    47641 ctaaagaggg ttggcaacat ggaataatag gaattgtagc atcaaagtta actgaaaaat
    47701 actacaaacc aacaattctt ctaggtatag aaaatgggga ggctactggt tcagcgaggt
    47761 caataaaagg atttaatata tttgaagctc tcataaagtg caaagacttg atgactaagt
    47821 ttggtggtca tgaacaagca gcagggttat ccttggattc agacaatgta gaaattttag
    47881 caaatgaaat aaataaattt gcagattata atttaactga agatgatatg atagagaatg
    47941 taaatgttga gtttgaatta caagaaaatg taataaattt aaatcttgta gaagaattac
    48001 ataagttaga accctttgga ttaaataatc ctaatcctag atttatagtt agaaattata
    48061 ttttaaaaga tttgaaagtt ataggtaaga atcaacaaca tctgaaatta agcatagaaa
    48121 aagagaaaag ctatgaatgt ataggattta atatgagtca tttaaaatca atgtataaag
    48181 taggagacaa ggtagatgta ctattccaat tagatgaaaa taactatatg ggaaatagga
    48241 aagtacaatt tttacttaaa gatatacgat tggctcgacc taaaagtgca agtaatgaca
    48301 aactttcatt gaaattaatg tcaaaaataa taccaaaaga tactcaaagc ctgtataata
    48361 tatctgtgtc tgattttgaa ttatttgatg gaaatacaga tataaatata tttgattatt
    48421 ttgaaaaaga tacactaata atatctaatt ctataaatgg attttataga gcaatgtcag
    48481 atatatcact tatagatttg gattttaaca taaattacaa tattattgaa gatgttagca
    48541 aaaatactga taaattagag cttatatttt ctcctaatat tgataaaata gacttaaaaa
    48601 gatataataa tattattctt tatgattatt tatacaataa aggcgaatat tcgtatatat
    48661 atgagaataa aagagaagaa agtgaaatta taaaatatta taataaaaca gacctattat
    48721 atttaaagaa tgtagttagt aatatagttc catcaagaga tgaatttata acaatttata
    48781 aacaagcact cataaaaaaa gaaattgatt tggatatggt aaatataagg gaaactttta
    48841 atgtaattcc attaaagttt tttacgatat taaatgtatt tagagaatta aatttacttg
    48901 actttaattt aaattatgaa aaaaattctg tactcataag aatattgcca aaaccacaga
    48961 aaaaattaga cttaaatgag agtttaatac tcaataatct aaaaaattta gaaaaacaat
    49021 acaatagcag ttattaggag gcgtttccca tggatttaaa aaactttata aggaatattg
    49081 atgatttccc aaagccaggc atagatttta aagatgtgac aactctattt aaagatggag
    49141 atgcatttaa atatgcagtg gattctatag ttgaagaatt gaaagataaa gatgtagatt
    49201 tagtaatagg accagaagca agaggatttt tgatgggtac acctgtagca tatgctttag
    49261 gagtaggatt tgttccaatt agaaaacctg gaaagttacc aggtgaagtt gagagttatg
    49321 agtatggatt agagtatggt acagatactt tagagataca taaggatgca attaaaaagg
    49381 gtcaaaaagt agcaatagta gatgatttat tggctactgg tggaactatg gaagccgctg
    49441 ccaagcttgt agaaaaatta ggtggagaag tagtttctat gcaatttttg attgagctaa
    49501 aattcttaaa tgggagagaa aagttgtcta actatgatgt taattctctt ataaaatact
    49561 agtactttta taatagttgg tataacacca actattttca tatatattaa aattttatac
    49621 aaaataggtg gttatatgca tgataaagaa ttacaagaaa taattgaaaa aataaaagtt
    49681 tatgcaccta atgcaaattt tgaaatggtt gaaaaagcct ataatcttgc aaaaaaagcg
    49741 catgaaggac aatatagaaa atctggggaa ccatacttta ttcatccatt agcagttgca
    49801 aatattttaa tagagatgga attagatatt gaaactataa cagcaggttt acttcatgat
    49861 gttgtagaag atacagaata tacctatgaa gacataaaaa aagaatttgg caaagaagta
    49921 gcggatttgg ttgatggagt aactaaactt ggtcaaataa aatatcgttc gaaagaagaa
    49981 actcaatctg aaaatttaag aaaaatgttt ttggcaatgg cgaaggatat aagggttata
    50041 cttataaaac ttgctgatag attgcacaac atgagaacat taaaatacat gcctccagaa
    50101 aaagcaaagt ataaagcaaa agagacactt gaaatttatg gtggtatagc tcatagatta
    50161 ggaatttcca agataaagtg ggaactagaa gacagagcat taagatttat ggacccagaa
    50221 gggtattatg atttagttag tagagtatca atgaagagaa gtcaaagaga ggattatatt
    50281 caaggaattg ttgaacttct taaagataag tttaaagatt taagtatacc atgtgatgta
    50341 tatggtagac caaagcattt ttacagtata tatagaaaga tgcaaaaaaa acataagact
    50401 tttgaagaga tttttgattt aacagcagta aggatattgg ttgataatgt aaaggattgc
    50461 tatgcagtac ttggtatagt tcatacatta tggagaccta ttccaggaag atttaaagat
    50521 tatatagcaa tgccaaaacc caatatgtat caatcattac acaccactgt tgtaggtcct
    50581 gatggagaac ctttagagat acaaataaga actcatgaga tgcataatat agctgaaaat
    50641 ggtatagcag cccattggaa atataaagaa ggtatctcta gcaaagaatc aaaggttgaa
    50701 gaaaaacttc aatggttgag acagatgatg gagtgggaaa aagatttaaa agacccacaa
    50761 gagtttatgg atgcacttaa agaagatgta tttaatagtc aagtttatgt atttacgcca
    50821 aaaggagacg taattgaatt accagcagaa tcaacaccta tagattttgc ctatagggtt
    50881 catacaaatg taggcaataa atgtgtagga gctaaaattg atggaagaat agttcctatt
    50941 gattataagc ttcaaaatgg taatattgtc gaggtactta catcatctaa ttctaatgga
    51001 ccaagtaggg attggcttaa tatagtgaag accccaaatg caaaaagtag aattagacaa
    51061 tttttcaaga aggaaagaag agaagaaaat gttgaccgtg gaaatacaat attagaaaga
    51121 gaatttaaga agcatgatat accacaaaaa gaccctcttg ttgaaaaata tatggcatta
    51181 gttgctaaga aatttaatca gccaagtgtt gaggacttaa ttgccactgt aggttatggt
    51241 ggtataatgt catctcaagt ggtatctaag gtaaaagacc tatatttaaa agaggttaaa
    51301 aaagctaaaa aggaacaaca gatacaagaa gaagatttaa ataaacacaa tataagtgaa
    51361 acagaataca gtaaaaagag aaagaaaaac gcatcacaag gaattatagt aaaagggctt
    51421 gacaatatct tagtaagatt tgctaagtgt tgtaatcctc ttccaggaga tgaaattgta
    51481 ggatatatta ctaagggtag gggagtagcc atacacagag ctgattgccc aaatgcaaac
    51541 aataatggtg atttcttcca aaatagattg gtggaggttt catggagttc agcaggaaaa
    51601 ggtaagtttg aagcggaaat tcaaataaaa gctattgata gaagaggaat tgttaatgaa
    51661 ataactcata ttgttgcaat ggataaactt agtttaaacg gaataaatgc aagaaaaggt
    51721 aaggatagta ttgtaagtat aaacttactt gtagaggtta atgacacaga ggaattaaaa
    51781 attctaatga aaaaattaaa agcaattcct ggtgtagaaa gtatttatag aatgacaaat
    51841 tagagttaaa ataataggga ggaaactatg agagcagtag tgcaaagagt atcttcatct
    51901 aaagttacag tagatgaaaa cactataggt caaataaata aggggttact agtattgtta
    51961 ggagtgactc atgatgataa atcaagtgat gtagattata tgattgataa aatattgaat
    52021 ctgagaattt ttgaagatga aaatgacaag atgaatttat ctctaatgga tataggtgga
    52081 gagcttttag tagtatctca atttacttta tatggagatt gcagaaaagg aagaagacct
    52141 ggatttagta atgcagctag gccagaactt gcaaataacc tttatgaaga atttgtaaaa
    52201 aaagcaaaag ataaaggtgt gacagttggt acaggtcaat ttgcagctca tatgatggta
    52261 gagttaacta atgatggtcc agtaacaata ctattagact caagtaaatc tttttaaggg
    52321 ggaaaacttt aatgattata aaaaaatttg tagatagtta ttttggagta aatacttatg
    52381 tattaggtga tgaaaagact aaaaaatgtg cagttataga cccaggtgga agtttagtag
    52441 agacattaag ttttgtaaaa gaaaatgaat taaatattga atatattatc cttactcatg
    52501 ggcatggaga ccatatagga tatgttaagg atattaaaga aaaaactggt gctaaagtag
    52561 ttgcacatgt tgatgaaaaa gaattattaa atgacaaaaa taaaaatcta agttatacaa
    52621 tgagatgtgg agcgcaagag tttgatgcag atatctatgt aaatgataaa gacaagcttg
    52681 aacttggtga attaaaaatg actttcttgc atactccagg acatactcaa ggatgtatgt
    52741 gtataagagt tgaagatgaa atgttcacag gagatacttt gtttgctgga agtattggta
    52801 gaactgattt atatagtgga gactttaacc aaatggaaaa atcacttaag aaattatgta
    52861 aatatgaaga tgctgttaga gtatatcctg gacatggacc aaccagtact ctaggggttg
    52921 aaaagaagac aaatccttac atgtaatttt atattagtat ttaaaaaagt ataaaatatt
    52981 taataactat attagtgagg gggttcttct actgttaaat agaaggagtt aagttgatgt
    53041 tgtatgtttt tttaaaagga catgattata agtatgaagt agcagaactt ataaaattgt
    53101 ttactagtga atttaagttt aaagactcta atgctaacaa taatatagag tcaaagcatt
    53161 taataaatag attgatttat gagaatggtg tattattctc aagtactgaa tactatgaaa
    53221 atggaaactt aaagtacgag tcaatacaga atatagaaaa tatgaatctt gaatttttag
    53281 atggatttga tagcagcaaa acgattgaag aatttcttaa tagcctagat agtgaaagta
    53341 agaggaactt taaaaagctg tgtaaagaaa atatcaaaaa aagcatgttt ttagtgctta
    53401 aacaagtatt taattcttat gtaccatggg gaattttgac tggtattaga ccagtcaaaa
    53461 ttgtccacaa tttaatggat aaaaagctag atgataatag cataagaacc attctaaaag
    53521 ataattattt tataatagat gagaaaatag atttagctct tgaaatagct aaaagagagc
    53581 gattatttat gtatcctata gataaaaata aaatttctct ttatgtaagt attccatttt
    53641 gtccaactag atgtgtatat tgttcatttc cttcaaattc attaaaacaa tttggacatt
    53701 taaaaagaga atatgtttat aaattgattg aagaaattaa ggggtttgcc aaagttatta
    53761 aggatactaa gaaagaagtt gaaactctat atattggtgg tggaactcca actacacttg
    53821 atgaggaaga attggatttg cttataaatt cattgttcaa tgaattagat ttaagtaaaa
    53881 ttaaagaatt tactgtagaa gcaggtagac ctgacactat tacagaacaa aaactaagag
    53941 tgttaaaaaa acacaatgtt agtagaatta gtataaatcc tcaaactatg aatgatgata
    54001 ccttggtaaa gattggaaga gagcatagag tatctgattt agtagattgc tttaatatgg
    54061 ctcgtagaat gggatttgat aatataaata tggatataat tttaggtctt atagatgaag
    54121 acttaaatat ggtaaagaac acattggagg agataaaaaa actttctcct gaaagcctta
    54181 cagttcatac attagccata aagagagcat ctaacttaaa tataaatatg gataggtata
    54241 aagaacatct aacacaatat gaagaaatgg taaaaatgat agatttatca atgaaatatg
    54301 caaaagatat gggattaaat ccatattata tgtacagaca aaaacatatg ttgggaaatt
    54361 tagaaaatat aggttatgct aaagaaggat atgagtgcat ttataatata cagattatgg
    54421 aagaaaaaca aagtaactat gcattaggtg caggtgctat atcgaaattt gtctatgtag
    54481 atgaagatag aattgaaaga gtagaaaatg taaaaaatgt tgaacaatac attgaaagag
    54541 tagatgaaat gataaaaagg aagaaagaag aggtatataa aaatgttgac taaagctcct
    54601 agaggaacta aagacataac tccaaaagag gcttacaaat ggcgttatgt agaaaataaa
    54661 tttagagaaa tatgtgcttt atatggatat gaagaaatgg taacacctat atttgaacat
    54721 acagaacttt tcaagagaag tgttggagat actacagata tagttcaaaa agaaatgtat
    54781 tcttttaaag ataaaggtga tagagaaatt accctaaagc cagaaggtac agcaggtgta
    54841 gtaagagcat ttatagaaaa taaattatat gcagatactc aaccaacaaa attattttat
    54901 gtgacacctt gttttagata tgaaagacca caagctggta gacaaagaca atttcaccaa
    54961 tttggaatag aagcacttgg aagtgatact ccttcaatgg atgctgaaat aatcgcacta
    55021 gctgttcaat tttttaatga agttggatta aatgatttgg tagtgagtat aaattcagtt
    55081 ggctgtccag tttgtagaaa agagtacaat gctttattaa aagagtatct tgactcaaaa
    55141 gctgatatat tgtgtgatac ttgcaatgag agaagagaaa aaaatccaat gagggttata
    55201 gactgtaaaa atcctacatg taaagaaaat atcaaagata taccatttat tgcagaccat
    55261 ttgtgtgatg attgtaaatc tcactttgat aaacttcaag aatacttaaa ggaaatgaat
    55321 ataaactttg taattgataa aacaatagtt agaggcttag attactatag aaaaactgct
    55381 tttgaaatta tatctaatga tataggagct caaagtactg tatgtggtgg aggaagatat
    55441 gatggtcttg ttgaacaatt aggaggacca aaaggtataa gtggaattgg gtttggacta
    55501 ggaattgaaa gacttttact tacacttgaa ggtaatggaa ttgaaataga aaaccctcaa
    55561 tctacggata tatttatagt aactataggt gaagaagcaa atacaagaag ctttaaattg
    55621 ttaaaagact tgagacaaaa tcatataagt gcagataaag accatataga gagaagtgtt
    55681 aaagcacaat ttaaatattc agataaaata aattcaaaat ttacaatagt aattggagat
    55741 gatgaactta aaaatgacac tgcgacatta aaaaatatga aaacatcaga gcaaactact
    55801 gtaaaattaa gtacattagt tgaagaatta aagcaaaagc tgtaaaataa gaaagtatga
    55861 tgttttgtgt attagataaa tatatagcat taaaattaaa taaaatggag gtagtatctt
    55921 ggaaacttta aagggtttaa agagaactca ctactgtgga gagttgagag aaaaaaacat
    55981 aaatgaagaa gtcgtactta tgggatgggt acagaaaaaa agaaatttag gtggtcttgt
    56041 atttgttgat ttaagagata caagtggatt atgtcaaata gtttttgata cagatgtaga
    56101 taaagaagct tttgaaaaag ctgaaaaatt aggggctgaa tttgtaatag ctgtaaaagg
    56161 aaaagtatgc gaaagacaat ctaaaaaccc taatatgcca actggtgata tagaaatatt
    56221 tgcaactgaa cttagacttt taaacaagtc agaaactccg cctatttata taaaagatga
    56281 tgatgatgtt tctgaagcac taagattaaa atatagatac ttagatttaa gaaaaccatc
    56341 tatgcaaaga aatttaaaat taagacataa agtaatgaat attacaagaa attacttatc
    56401 aaacaataga ttttgcgaaa tagaaacacc atttttaata gctccaacac cagaaggagc
    56461 aagagattat ctagtaccaa gtagagttaa tccaggaaaa ttttacgcat taccacaatc
    56521 accacaatta tataaacaat tattgatggt aagtggtatg gatagatatt tccaaatagt
    56581 taaatgcttt agagatgaag acttaagagc agatagacaa ccagagttta cacaaataga
    56641 ctgcgaaatg tcttttgttg agcaagaaga tgttatgagt atgattgaag gtttgcttga
    56701 agcgatattt aaagaagttt tagatgtaga attggcactt ccacttccta agatgactta
    56761 tgcagaagct atgtctaaat atggttcaga taaaccagat actagatttg ggtatgagtt
    56821 aacagatata tctgatgttg tttgtaactg tggatttaaa gtgtttgctg atgctacaca
    56881 accaggaaaa agtgttagag gtataaacgt aaaaggcaaa gctgacgact ttactagaaa
    56941 acaaatatca tcactagaag aacatgctaa gacatataga gcaaaaggtc ttgcttggat
    57001 gaaagtagga caagaaggag taacttctcc aatagcaaaa ttcttcaatg aagaagaaat
    57061 gaacgctata cttacaagaa tgaatgctga agtaggagat ttacttttat ttgtagctga
    57121 taaaaactca atagtatttg atgctttagg acaagttaga cttgaagtag caaataggct
    57181 taatctattg gataaaaatg tatataactt actatgggta actgagttcc cagtttttga
    57241 agaagatgaa gaaacaggca cattctcagc aatgcatcac ccatttacat cacctatgga
    57301 tgaagattta gacaagttag aagaaggtga taagtcatct ttaagagcga aagcttatga
    57361 tattgtattg aatggttatg aaataggtgg aggaagtgtt agaatatcaa attctgatgt
    57421 tcaatcaaga atgtttaaag cacttggttt tacagaggaa agagctaatg aaaagtttgg
    57481 ttatttatta gaagcattta aatatggtac tcctcctcat gctggtctag cttttggtct
    57541 tgatagactt gttatgttac ttgcaggaac tgataatata agagaagtta ttgcattccc
    57601 taaaaatcaa aatgctgttt gccctatgac aaatgctcct acattggcgg aagatgagca
    57661 attagaagaa ttaagtataa aagttgatat aaaagataat gaataatatg tagttgcaag
    57721 aaactaaaat gtaaaaagtc tggatataaa atatatttat attcagactt tttttattag
    57781 aattatttta tattttttct ctatatattt tcataaatta tacttatatt cttaaccaat
    57841 ataattaaaa tactatgttt aaaaatttta cataaacttg gttgttttag aaactacttc
    57901 tgtaacgagt acacgatttt aattaaagaa tatataacaa atttgaaata agtctaatat
    57961 atgacttaat agattaaatt aaaataataa atttggacta tttatctatt tataacttaa
    58021 attatatcaa attgataaca aactagatat tatacttttt tgtagaatag aattacaatt
    58081 atataaagaa aaattctatt aatagtgaaa aaaaagtaga aaaaagtaga aattattctt
    58141 ttataaagaa agggagggat aatattgcaa gagagagaag aattagtttt aaatccagtt
    58201 ccaaaagagg aaagagttgg atggttagct ccattgttta atatgcttgg ttctaacata
    58261 gctatatctg aactaatggt tgggggaact ttaattttag gaatgactct ttctaatatg
    58321 attattacat caattatagg gaacttaata ttagttgcaa ttattatgat acaaggttat
    58381 ataggttaca aagaagggtt aaatacgtat gtattagcta agggggcatt tggagagata
    58441 ggaggaaagt atcttatatc tctattactt ggtataacta gttttggatg gtttggtgta
    58501 caagcagggg ttgcaggact atcagtacaa aagatattcc caagtgtaaa cctaacatta
    58561 gttacagtta tacttggatt atttatggtt gtttttgcac tgtatggttt taaagctatg
    58621 gctaaattta attacttagt tataccacca cttatgatat taatggtgtg gggagttttt
    58681 aaagcttttt ctacttatgg agttgaagct atttataatt atactcctca aacaactatg
    58741 agtatggttg aaggtcttaa tattgtagta ggtcttgtta tagttggtgc aattatatca
    58801 cctgaccagt taagatatac gagacgagta aaagacattt ggattatcag ttttttaggt
    58861 ctagggatta tttcattatt ccaacaagtt gcagctggtg taatgtctat gggagcacct
    58921 acttgggata taacagaagt attggctaat ttaggttttg gatgggtagc gtttgttatt
    58981 ttaatacttg catcttggtc tacaaacgta tcaaatgctt attctggtgg attagcatta
    59041 aaaactattt ttccaaatgt aaaaagaaat gttttgactt tagttgcagg tctaataggt
    59101 acaataatag cagctactgg tataatattt aaatttcagt catttttatc tttcttggga
    59161 atcgcagttc ctgcaatagc aggaattatg tggtgtgagt attattttat acaaggaaga
    59221 acttataagc atagagaagg aataaattgg attgcagtaa taagttggtt aataggtttt
    59281 gcagcgtcgt attatagttc aaaaataaat ttcttaatac ctccgataaa tggaattgta
    59341 gtgagtatgg ttatttatta tatattaatg aattgttttg gaatcaaaga taaataaagg
    59401 gggcaattac atgggtcgta aggtgaaaat aggtataatt caacaacaca gtgtacttgg
    59461 aaatgttaaa aaaaatatag aaaaagcagt agagatgata gatgacttag gaaaacaagg
    59521 ggcagatata atttgtttac cagaactatt tgctacagga tataatttag agtctttagg
    59581 tggagttaaa acattagaat taataagaga acataataag tacatagagg aatcaatgtc
    59641 agaagctgct aaaagaaata atgtatacct gatatctcca tatggaacct tagaaaaagg
    59701 gtctacacat gtttataatt cagcagttat atttgataga aaaggtaaga ttatgggaga
    59761 atactgtaaa aatcatctgt ggtcattaga agcagtatac tttaaaggag gggaaaaagt
    59821 agaggtatat gatgctgatt ttggtagatt tggtgtaatg atatgttatg atgcaggttt
    59881 tccagaagta tccagagaat tgacattaaa aggttctgaa ataatattta taccatctgc
    59941 atggagaata caagatgaag atatgtggga tttaaatgta tctcaaagag ctttagagaa
    60001 cactgtatat acagtaggtg taaatttagt aagtaatgac tctaatttaa tactatttgg
    60061 aaagagtaaa atatgcaatc ctagaggtac tgtaataact caattagata catacaggga
    60121 aaaatatgta ttatcagaga ttgatttaga tgaaataaaa aagtataggg aagatatccc
    60181 ctatttgaaa gacagaaaaa gtagctataa catataattc tatactataa tacaagttca
    60241 atataaaatg ataaaaagag gatgtatcaa tattaagatt aaatttatta tactttgata
    60301 tatcctcttt aatattatca actttgattt aaatattttt aaaatagttg ataagaaagt
    60361 ttttgaacaa caacacagct ttagattcat agttttcgct aaaccatgaa agatttaaat
    60421 atcttttaaa ttcaatatct ttgatatgta acaacttaat ctcagggtct tgagataatc
    60481 cccatgattt tccacatata aatccaactc cttgaagtga ttttataaga gcataaattg
    60541 tatatggtga atcagactca aatgctattt tgggtttgaa attagctgat tcgcacagga
    60601 tatcaattac ctctctataa ttctcaccct tcgttataac tataaagttt tcatctgaaa
    60661 cttcgcttag atatacttca tcctttaatg ataaaggatg gtttattgaa actcctaata
    60721 gtatttcttc gcatgttaaa gttatgctat tttctgagtt tagctttgta aatgaagaag
    60781 atatgtacaa atcaaaatca ctttttttat aagatgatgg caaagtatgt gacacattaa
    60841 aagtaatatt gggatatagt tttctgaaat tactcaaaag tgatggtaac acattagccg
    60901 ctgcaggaga taatatcttt acttctcctg aattttccaa agaagtgtct tgtaactcct
    60961 tttttgaact ttctactaaa tctaaaatgc tatctactct ttttagaaac aatcttccat
    61021 actcatttaa aacaatatat cttccttttc tgtcaaataa ctgaactcca agttcttttt
    61081 ctaaggaaga tatagttttg cttaaagctg gctgtgctat atttaacttg tgtgatgctt
    61141 ttgttatgtg ctccattcta gcaacagttt gaaaatactt aagatgcaat aaatccatag
    61201 tataaaatac acctctttta atatgacttg agttatatca attttataat caactccaca
    61261 ttatacatta taatataaag tttatataat aaactacaat atattaaaaa gttatttaaa
    61321 ttgttattta tattttgtac ataatctaat taaaaaaagt aactttaaat ggttaactaa
    61381 ggaaatattg ttacaaactt tagttatata aaaaattcgt ttgatgagca aagtattaat
    61441 gtcaagtaag aataaaagct tattagataa gaaaaataat attttataat aatgatgtta
    61501 cttaaattat tatgttatac tattttaaat aggaaacatt tagatactat aattttaggg
    61561 ggagtcaaga tgaaattaac taaatcaata tcatcaatgc tgtgcttatc tctagtttta
    61621 agcttatcac cagtagcata tgcaaatgaa ataagtagaa caacagttga gaatacttta
    61681 gtaggtcagg atagatatca aactgctgtt gaagtaagta aaaaaggatg gagtagtgca
    61741 aatgaggctg tagttgtaaa tagtgaatct attgtagatg cattagctgt aacaccattt
    61801 gctaaattaa aaaatgctcc tatattattg acagaaaaaa ataatctaaa ttcacaaact
    61861 gagaaagaat taaaaagatt aggagttaaa aaagtttata taattggtct atctagttct
    61921 gtatcaaatg atgtgcaaaa acagttagaa tctaataatt taactactga tagagttggt
    61981 ggtagtgata gataccaaac agctttgagt atagctaaaa aaatagaagg attaaaagat
    62041 atatcagaaa tagctgtagt aaatgggtac acaggtcttg cagatgcagt tagtatagct
    62101 tctgtagctg ctactaatgg aatggttata cttccagtat ctgatgcatc tggtatatct
    62161 agctttaaag attttataga atctaagaat ataaataaat catacataat aggctctact
    62221 aatgctgtat cagataaaat aaaacaaagt ttacctaatt cagaaagaat aggtggagca
    62281 gatagaaatg aaactaatgg taaagtaata gaaaagtttt atacatctaa taaattaaac
    62341 aatgtatttg tggctaagga tggaatgaaa aatcaaaatc aacttataga cgctttagca
    62401 gtaggagtac tagcatcaaa agaaaattct cctgtactaa tcgtaggaga acaattaagt
    62461 gcaacacaaa agagtgtatt ttcgaataaa aatgctacct ctataactca agttggtagt
    62521 ggaggaaatg aaaatgcatt taaagaatta aaagatttac aggcaaatag taacaataag
    62581 agagtaatgt atgtaactaa tacagataag gtaaatataa gaaatgatgc tacaatagaa
    62641 gcttctgtta taggatatct aaataatggt gatgaagtag aagttttaga tgtgttaaaa
    62701 actggatggg tagaaataaa atacaatgaa ggtattggat atgttagtgg gtcttatttg
    62761 acaaataata agcctgataa tagtaatgaa aatataaaaa taaagtatgt aaaagaaaaa
    62821 gatggtctaa atgttaggaa aggaccaagt acagaagatg aaaaaatagg tcacctttca
    62881 tatggaagca aagtagaaac aatagaaatg tttgctactg gatgggtaaa aataaagtat
    62941 aatggtggat atggttatgt aagtaatgat tacttatctg atacaccagt tatttaatta
    63001 taagtaacaa taaaaaaact gctttaattt gtaaagcagt ttttttataa aaatttaagt
    63061 ctaataacta tttctatttt gataccttaa tcctgggatt tattattatg gatattatca
    63121 acatacaaac actccaaatc cagtgtctaa ttccaaactt agatattaat aaattcagtc
    63181 ctgctatggt aaataagcag tatttaatta caattaaaag tgtatttata gaaagcccct
    63241 tcatagaaag tatcctaaat ccaataatag acattctaag tcctataaat gccaataata
    63301 tacctgcaat atcctgaatt aagtaatagt attcaaattt catattcaat caactcttct
    63361 ctgagcagta tcaaatattt ctaactcttc aatttcatca tctgtgtatg taagcatagt
    63421 aaaaccagtt acaccataaa taaatgaaat tataggtgta atccaactta agaaacaata
    63481 tggaataaat tctagtgggc ttacaccaag agtctgagca cagaaaacag catttgaatt
    63541 ccatggtatt aatggaccac caagtgtagc tgtatcttct ataattctag atagattttg
    63601 tggttttaat ttaaattctt tgtatattgg agacattaga gttccgacaa atacagaagt
    63661 gaacatcatt gatgaactaa atgcatttgc aaagtaactt actataaaag tagacccaac
    63721 caatttagtt cttgattttc ctattttagt tataagaggt tgaagcagta cttctaagaa
    63781 tccagttttt tggagaatac cagcaataac gaaaactaca aatactagta aaacggtttc
    63841 agccatactc attattccac cacgatttaa tagtttgtcg gcatagacaa atccagtatc
    63901 tatagtaaat cctgatagca tgtaatttaa aacatcacct attttttctc cttcttgaaa
    63961 tactgctata ataagtccca tgatagctga acttagtata gatataatgg gtggcttttg
    64021 tagcaataaa agtaataata aaaatattat tggaatcatt gctactaaac caatatgaaa
    64081 gtttgaattt aatacatcct tgatttgatt tatttgaaca tagtctatag tattatttga
    64141 atacttaaat ccaattactg tatacagaac tatacatatt acgtatgcag gtcctgtggt
    64201 ataaaacata tgcttcatat gtgtaattac atctgtctta caaactgctg cagctaagtt
    64261 tgtagaatct gatagaggtg acatcttatc tccaaagaaa gcaccagaaa tgactgcacc
    64321 agctgttaaa ccaagaggaa tacccatact agtaccaata ctcatcattg ctataccaga
    64381 agttccaaga gtcccccaag atgtaccagt agcaacagaa gttaatgaac atacgattaa
    64441 tgttgtaagc agaaaatatt taggggttat tattgttagt ccactatata ctactgttgg
    64501 tattgtacca gcagctatcc aagctgctat tagtacacca acagaaagta taattaagtt
    64561 tgattgaaag gcatctttac caacttcaaa tccaaaagct tctatttcat cattagtata
    64621 accaagtttc atagctgcag gaactactat aagccatgaa agtaaaaaca ttgtagttat
    64681 agaagcatta aatacaacta taccaactga tgttagagct attattgcaa gcattactaa
    64741 caaggaatat ctaaatgaag gtatttttct ttctgtattc attttcatag tgcaaattct
    64801 ccttaaatat ttttagttaa ttgcattttt tattcttata agagcttctt caagcatact
    64861 tcttggacaa gctaagttta ttctttgata cccacttcct ccaatgccaa agctgtttcc
    64921 ttgatttaga gcaactttac ctttttgtac aagtatactt tctagttcct catcacttaa
    64981 tccaagtgca ctaaagtcta cccataataa ataagtacct tctggttttc taactttaag
    65041 ttttggcata ttttcattta tatattttat agcaaaatct atattagatt ctaaatattc
    65101 taaaaatgat tctagccaag attcaccatt attatatgaa gcttcagttg caactaagct
    65161 aaagcaatta tttctcttta tatcaattct ggtaaaggca tcatctaaca atttatagtc
    65221 tttttcatca ggtagtatta catatgatga ttgaagacca gctatattaa aagttttagt
    65281 tggagccata caagtaattg tatttttttc aaattcttta gaaatagatg ccataggaat
    65341 atgcttatgt ttttttaata taatgtcact atgtatttca tcagatatga tttttacatt
    65401 atgcttaaga caaatatctc ctagtttttt taattcatct tttgtccata ctctaccaac
    65461 tggattatga ggattacaaa gtatgaataa ttttacatct ttaatcttgt tttcaatgtc
    65521 ttcataatcc attatgtagt ttccattttc tagtttttgt aatgggctta taattagttc
    65581 tctattgtta ttttttacaa cactattaaa agggctgtaa actggttctt gtatcattat
    65641 tttatcattt gcttttgtga gttcatttat taaaagactt atagctggga taacacctgg
    65701 actataaatt aaccactcac tttttatttt ccagttatgt ctcctatcta gccagtttac
    65761 tatagattca ttgtaagaat ctggtcttgt tgtgtagcca taaatctctt gttctagtct
    65821 attttttaag gaatcaatta tacaaggtgc agccttaaaa tccatgtcag ccacccacat
    65881 aggaagtaaa tcattagttc catacttttt ttccatttct gaccatttag atgaaaaatt
    65941 attgcttcta tcaacaatct cattaaaatt ataattcatt gtaaatacct ccagtaattt
    66001 taattttatg ttaataatat taagcaaaca acgtgccaat attgataata aaaaatattt
    66061 attcacaagt taatatttaa tattaagtaa tttcagagca tagataagat aaaaatatta
    66121 attatactta aaactttgtg gaaaaggtta tttctaataa atttttaaat atatgaaaat
    66181 gtaattaaat aggttaaata ggtttaactg tttaacttgt tagacctatt tatatttttt
    66241 agaaattcta ctccttttga tgttataatt gaacctccac gaccaacaag tatgtctaca
    66301 agcccaaagt tttgtagttc taaaagcata tttctaatct gttgttctgt caaaaatcta
    66361 ttttcctcta aagctatttt acataagctt tttctaccta ttctttcttt taaatcataa
    66421 gcctttttta aattatccaa tataaatatg tattcatcta gtgacctttt aaaattatag
    66481 tcgtatttca tgatgttttt gtctttgcca atgttgaatt gatacttttt tatattatca
    66541 cttactttat tacaaacggt tctactataa tttgaatcta tagtatctaa tatatattca
    66601 ggtaaatcac atatttctat aatatctttt tccaagtaac agaagtattc tcctaagttt
    66661 cttaattctc ttacatttcc ttcccatcta tacattttaa agatttcttt tacttcctca
    66721 cttagaataa aattacatgg aatatcatat tttaatgaac caaaaatttc aaatatatca
    66781 gcaggtcttt ccctaagtgg atgtatttta aggggaagaa cattcaacct aaaatataaa
    66841 tctcttctaa acatattttt agatacaagt tcttttaaat ttctattagt agctgcaatt
    66901 attcttacat caatgtcaat gacattatta gaaccaatac gtcgtacttg tttttcttgt
    66961 atgactctaa gtaatttaga ttgagaattt aaatccattt ctccaatttc gtctaaaaat
    67021 atagttccat tgttggctaa ttcaaataaa ccaattttcc cacctttttt agcaccagta
    67081 aaagcccctt catcatagcc aaataattca ctttgcagta gattttcttg aaaagtacta
    67141 caatttactg cgacaaaagg accatctttt ctcctagaag cactatgaat ggattgagca
    67201 aataattctt taccagtacc actttcacca attataagga tagatgaatt tgattgagcc
    67261 attttattag caattttttt tgtattaata gtttgtatac tagaacctaa tatgtcatca
    67321 aatgtgtatt tactgatatt accagagttt actagttgtg ctcttaattt agcttgcttt
    67381 ttttctaatt gagaaaactt tgtcatcttt agtataaatc catcaaatac atttaattgt
    67441 atatgtttaa tctctaaatt tatatcaata tgatttattt taatcaattt ctcaaaaaat
    67501 ggagttttat tggtcaatat gctttgaaaa ttgatgtctt ttatgcaatc accaacaaat
    67561 gagtcaatca tttcatttgc atttatagac aaaatttttc tagccatatg attatagaat
    67621 tgaattattc catcgttatt tgtgcatata attccatcat caattataga tagcaacaaa
    67681 tcaaattgtc tttcaaactt atttgcatct aacaataact cacctgtact ataacttgta
    67741 ggaattattt tttctacgta tttttttatt aggtcatctt ttattagatg ttttaatttg
    67801 gtttttgtag caatttcaac aatacaactt aaatctatta ttctgtattt taaatctata
    67861 acttcacaat ttttaaactt aggcaagatt ttttcgccag gagttaggat tacagcatct
    67921 gtgggagtaa actcaatctc tggatagcag ggcaggatat ttatattgtc tattcctaat
    67981 ctatgtatta aagaaattgt ttctattgcc atatctttac ttaagttata tacataaata
    68041 attttatctt tttctaattt ggatatcata tcaatactgt tcttttcaaa tgtcaactta
    68101 ggaacaatga tttgagcatc atagtgagat aattttacta tttcatcata ttttaaataa
    68161 gcagatagga gtataagttt ttctttaaaa aactttaaat ttccctcttc aaatgagtat
    68221 aaatttatct ttagattatc acctaagaat tgccttattt gattttcata aatttctcca
    68281 gcgtcttttt ttaaacttac aatagctaat tccatttgtt tcattttaac ctctcataca
    68341 aatttgactt attttagatt acataataat taaattagat taaataatat tatttaatta
    68401 ttattataaa tttattatag actacacatt gaagtaaaag aagtagctaa aattattcct
    68461 tttatttttg ttttttgtat ctatttggta taatagtatt agagcgtata ataatttagg
    68521 caaaattttt taaggaagac tagaggagaa tataaaatga aaaaatgtct ttactgtaat
    68581 agggatataa agtcaccaaa taataaatta gctataaaat atgatgacaa tatgaatata
    68641 tatccatgtc gtgcagaatg taaggaaaaa attgaagaat atatagcaaa aaaacctaca
    68701 tataagttta taaatatatt agaggttctt ttaggtgtgg gcggaatatt ctgctttttg
    68761 agtaaatgga ctattgcagc ccaagttgta cttggaattg atgcactggt tatattttta
    68821 ttcccattgg caggatttaa aatgaatgga aagttaagta tggaacaaac taagaatcaa
    68881 agtaggtcta ttgcaatatc aattttggca tttatagtaa taataactgt attatatttt
    68941 aaacaagttg gtaagtagag cataattgta taagacatca ttagagtatg aggtgagttt
    69001 atggaagaaa gaaaattagc accaaacaat gagagggaag ctctcataaa aagattaaaa
    69061 agaatagaag gtcaagttaa gggtatacaa aagatggtgg aacaagaacg ttattgtgtt
    69121 gatatattgg ttcaaatatc tgctataagg tctgctataa atagagtagg cacaattatc
    69181 ttagagaatc atataaaagg atgtgtagca gaaagtatta aacatgatga tgctgataaa
    69241 actgaagaga tgataaaaga gcttatggat actataaata aatttacaaa ataaggaggc
    69301 ggttagttat gaatcaacaa ggatttatta tggaaattgt tgatgataga actgcaaagt
    69361 taaaaatgca aagacattca gcttgtgctg cttgtggaaa atgtgctact acaaattcag
    69421 cagagtcaaa agaaatatta gtagaagtag acaacacaat tggtgcaaaa gttggtgacc
    69481 atgtagaagt gagtatggat aatatgaatg tattaaaagc agctgtaatg gcttatataa
    69541 tacctcttat agcacttctt gtaggaacaa tagggactta ttatatattg ggggcaattg
    69601 gaatgacaac tggagttgaa gccataagtg gattagttgg aatttgtctt acaataatct
    69661 gttatttata cttaaagaag aatgacaaaa aatttagaga cagtagagaa tttataccag
    69721 taataacaag aatattaatt gacctttaag ataattataa ttaaaaaata gcaatgtact
    69781 ttttaaataa atactcaata cgtatttgta gagtttaagt acattgctat ttttatattt
    69841 ttattttgat tttctgtaag gaataaagtt ttttctgatt tttaaaagat tcaatgttct
    69901 tacaaaagaa gttacaacta atacaccaag tgctattgca gcacttagaa atagcgcatc
    69961 tgtacctttt gacatagcca aagaataatt tttctgaatc aaagacaaca ttgtattata
    70021 tagcccaatt cctggtatta gaggaagaat acctggaatt acgaacacaa tagctggttg
    70081 ctttagtttt cttgctaaag tctcactgca cgctgagacc aaagcagcag cgataaaacc
    70141 tgcaaaaact gcatttgtag taaaattaaa taaataaaca tatacaatcc aaccaattcc
    70201 gccagttact ccagctggaa gaagtagata tagtggagca ttaaagaaaa cagcaaatcc
    70261 agcagttgca acacctgaaa ataaaaaatg caaaatcata gacatatcag tcattagaaa
    70321 ggacctccta ttctatacca tgtatcaagg acaaaaccaa ctccagaagc aattgcaata
    70381 ataatcatag cagcatcaaa agcacgagaa attcctgata ttaaattccc tgatattaaa
    70441 tctctaatag cttttataaa agcaactcct gggagtagtg gcattatagc gccgactata
    70501 agcattttag ggtcatctaa aatgcctatt tcaactagta gaactcctga aagtgcaatt
    70561 aaaaaagaag atactaaaca ggaaaatgca gatattgcac ttatcttcat cgttttatca
    70621 taagtaattg cagcaaagat tgctattata aacgtaagta taaaatttaa tacataattc
    70681 cctccaagca aacaagcaaa acaagcagat gcaattcctg tacaagagta aaataaccat
    70741 aaaggataag gttttacatc ttggatttca taaagacgtg caatggcatt atcaatatca
    70801 tactctttgt cactaacaaa ttctcgagaa aaactgttta ataaagaaat cttatttaag
    70861 tttatacctc ttgattgaat cgttttcata aacgaaaagc catcaaattt ctcatctgaa
    70921 ataattatta ctgtaggagt tgtaaaaata tttacatgat aaaaccctct tgacctgcat
    70981 attctaagga cactgtcttc aaccctagat gtttcagctc cattttttat catcagttcc
    71041 cctacaaata gagctagttt tagaatattt ttcttatatt caagttcctc attttgattc
    71101 ataaatacac ctccaaatta aaaaacgtaa tattgaaatt atatcaaatt tttaagtaat
    71161 attccttaat atctatacaa atatattatc ttttttttgt ttcaaaatag tcacatttaa
    71221 attatatcca attaaaagaa ttagacaata ttatttatta tattttaact taaaaataaa
    71281 gaaagtaact atgttaaata tggaaaaatg gcaaaacact tgactattaa ttggtaaagg
    71341 actataataa taaattatca tattgataaa aacgaaaatt atgaaatgtc tgtaaaaata
    71401 aaatttaaat aataactagg aggagtaata tgtttgagaa cttgaaaaaa gcggaccctg
    71461 agatttatga aagtatgaaa agagagttaa aaagacaaca aagaaatatt gaattaatag
    71521 catcagaaaa catagtatca gttccagtaa tggaaactat gggaagtcat cttacaaata
    71581 agtatgcaga aggttactca ggcaaaagat attatggtgg atgtgaatac atagatgaag
    71641 ttgaaactct tgcaatagat agaatcaaaa aaatatttgg agcagaacat gctaatgttc
    71701 aacctcactc tggagcaaat gcaaatattg gtgtatattt tgctatgtta aaaccaggtg
    71761 atgttgttat gggaatgaat ttatctcaag gtggacattt aactcatggt gctccagtta
    71821 atatttctgg tcaatattat aaattttatg aatatggtat agataaagac agtggactta
    71881 tagattttga tgaagttaga aagatagcac atgaagtaaa acctaaaatg atagttgcag
    71941 gagcaagtgc atatcctaga gaaatagatt ttaaaaagtt tagagagata gctgatgagg
    72001 ttggagcatt gcttatggta gatatggctc atatagcagg tcttgtggct gctggtcttc
    72061 accaaaatcc atgtgaagtg gctgattttg ttacaacaac aactcacaaa actttaagag
    72121 gacctcgagg gggtgttatt ctttgtaaag aaaaatatgc taaagacata gacaaagcaa
    72181 tattccctgg tattcaaggt ggacctcttg aacatataat tgcttctaag gcagtttgct
    72241 ttaaagaagc tttaagtgat gagtttaaag aatatcaggt acaagttgct aaaaatgcaa
    72301 aagccttagc tgaagaactt attaaaagag attttaaatt gatttctgga ggtacagata
    72361 atcatttaat actattagat ttgactaata aaaatataac tggtaaagca gcagaaaaga
    72421 gattggatga tgcttatata acagctaata aaaatacaat tccatttgac ccaaatggtg
    72481 ctcttattac aagtggaata agacttggta ctccagctgt aactactaga ggtatgaaag
    72541 aagaagatat ggcaataatc gctgaggcta tagacctatg tttaacttat gatgaagaat
    72601 ctaaggctag aacattggtt gttggtttaa ctgaaaaata tcctttatat gaagaatata
    72661 atattatgga ttaattataa agaacaacta aataaaatac atttaaataa ttacaatgat
    72721 tgtgtatgta tttgaatata gttagatata aaaaaggtgt ctcaaaatga actttttagt
    72781 ttatgaggca cttttttgta tggaatcgaa catatttgtg ttatttaaat aataaaaaat
    72841 atggttatct ctatttttag atggtctctt tgtatttttt aattaaaact ttaagatttc
    72901 ttaagaatta tactaggttt ttctaaagat ttaaagtcta tacttataag tatagtaatc
    72961 atatgaaaag aaaagcttta acgaggaggg aatcttatga aggtattaat tcttacagga
    73021 aaatttggta tgggtcatta ctcagcatca aattctttaa gtgaagatat aaaagcaaaa
    73081 tttgataatt cagaaataat aattaaagat atatttgagt acattatgcc taactattca
    73141 gataaaatgt acaagacttt ttctattctt gtaaatcggg gaagtagtct ttataattta
    73201 ttttataaat gtgctgaaaa tggaaagaaa gatataaaat ttactttttc agattatttt
    73261 ttaaataaat tagatacatt acttcatgaa gtacaaccaa cagtagttat ttcaactttt
    73321 ccattttgtt ctcaattagt atcaagatat aaggaaaaat ataactcaaa tcttccttta
    73381 ataacatgta taactgatat aagtagtcac tcagaatgga ttagtaaaaa tacagactgt
    73441 tatttagtag ctagtaaatc tactaaggaa gaacttgttt ttaaaggaat agatgaatct
    73501 aagattaaag ttaatggtat tccagtaaaa aaagaattta aaagaataga acatgtaaat
    73561 cattcaacta aaaaaaatat tcttataatg ggtggaggat taggattact gcctaaatca
    73621 gaacaatttt acaaagaatt aaatagttta gaaggagtaa aaactactgt aataactggt
    73681 aataataaaa aaatgtacta taaactatat ggtagatatg aaaatataga ggtagtaggt
    73741 tacaccaatg aagtatataa atatatgaaa gattctgacc ttattatttc taaaccaggt
    73801 ggaataacac tatttgaaac aatttattca gaacttccaa tattggcatt taatccattt
    73861 ttacaacaag agatagataa tgctagtttt atattaaata atgaaattgg gagaatactt
    73921 ggtaaaaata aaaaatacta tgtagatgag ataaaagatt taatttatga tgatgctact
    73981 cttaaagaaa tgagcagtaa catgaaagaa ttaaaaaaac aatttgataa taatacctta
    74041 gagaatattt tattttcact tgatgaacaa ggagcgtgta gagaatgtat gtagtaggtt
    74101 taattatcgt tgttgcattg atttttttag tccattcaat tatacctaca tactataata
    74161 aactattaaa taaagaggta ttaaaaaata tggctggaga aaatgaaatt gcacttacat
    74221 ttgatgatgg tccagataag agatatacag aaaaattact agatgtgtta aaagaaaatg
    74281 atatacaagc tatgtttttt gtagtagcaa agaatgctga aaaagagcct gaaattataa
    74341 aaagaatgtt gagagaaaat catatagttg gtttacattc attggaacac agaaatgcat
    74401 ggctttatag ttatagttat gttaaaaaag attttattga aagtacaaat ataatgaaaa
    74461 atttaggtgt agatgtaaac tactatcgtc caccatgggg tcatactaat atattttcaa
    74521 attcatttgt taaaaaatat aatctaaaaa tgactttgtg ggatgtaatg gcagaagatt
    74581 gggagaaaga tagtacagta gacattatta taaacaagtt aatgagtaga acaaaagaaa
    74641 attctataat ttgtttacat gatgcaggtg aaaattcagg tggtgcagta ggtgcacctg
    74701 agcgtactat tgaagcttta aagatagcta ttccaaaact taaagccagt ggtttaaagt
    74761 tcgtaacacc agaaaggatg tagttatgat aagtaaaact gaaaagaaaa ataaatgcct
    74821 aggtagtgta gcttttttag ttttactaat gggcataaca ggatattttg tttttagagg
    74881 gcagtcagtt gaatcattga taaaatcatt aaaaggtgca agccctatgt ttatactaat
    74941 tggatttgca atgatgttta tatatgttgc ctgtgaaggt ataaatatat acttaggtat
    75001 gaaagcttta aaccaaaaga ctactctttt aaaatgtatg ggatatgctt ttattggatt
    75061 ttattttagt tctattacac cttcagcatc aggtggtcaa ccagcacaag tttattatat
    75121 gaaaaaagac gatataaata tatcatattc ttcacttata ctattagtca ttgttgttat
    75181 tcaccaagta gttatactgg catatagtgg aataatgttc attatggaac gtgagtttat
    75241 attaaataat gtaagtggta tgaacatttt actaatatat ggtgtaataa caaatgttgc
    75301 tcttgtcatt ggagtaattg caattatatt ttcaaaaaaa cttgtaaaca attttataat
    75361 atcaataaca aatttattag gtaagttaag aataataaaa gatgttgaaa gttcaagaaa
    75421 agttataata tctcaaattg aagagtatgt taaaggtgct caatatataa agcagaatcc
    75481 aaaacttgta gtccaaattt tagtcataac tattgtacaa ataacagcta tgtttttagt
    75541 tcctttcttt gtatacaaag cttttcatct atcaacatat actgtatttg agatattagc
    75601 gatacaatcc ttgcttaata ttgctgtttc ttcattacca ttgccagggg ctgttggagc
    75661 ttctgaaaat agttttatga cattatttaa gatatttttc cctggtcact tattagttcc
    75721 ggcaatgtta cttagtaggg gaataagttt ttatgcattt gtagctataa gtggattgat
    75781 atgtatagta gtacatgtaa aatcttctga aaaagtaaaa gcagtgaaaa aagttgctta
    75841 tgtaagataa tattatatag attattttta ttaatatgtc aatttgtgtg tatattttaa
    75901 ttaagcttta aacatattta caggagtaga aactattaaa acgtgtgaca ttgctaagct
    75961 tttgctaata aagtataata ttttatatag tttgaaatat ttaagaaata tagcaaaaaa
    76021 aggagcttgg tgatgaataa agagattcca aaaaaaattt tagtagctga tgataatcct
    76081 gagattaggg aaatcgtaga aatactttta actggtgaag gttatgaagt tattatggca
    76141 acgaatggag aagaagctgt aaacttagta gatagtacga ttgatttgat tattttggat
    76201 gttgtaatgc ctgtaaaaac aggatttgtt gcatgtaatg aaattagaga aaaaacgaca
    76261 gcaccaatat tatttttaac tgcaaaaact caagactctg ataaagttat tggattttca
    76321 gcaggaggag atgactattt atcaaagcct ttttcatatt ctgaactaat ttcaagagtt
    76381 aagtctctac ttagacgtta ttatatatac cagggaaagg aaaaggaaag agagccaaat
    76441 atattaaaaa caaaagagtt atgtgtcaat ttagatactc aagatgttac actaaatgga
    76501 gaacctgtgt ttttgacaac tatagaatat agtatattaa ttcttatgtt gaaaaataga
    76561 aaaaaagttt tttcgtctga aaatttatat gaaactattt gggatgagca gtatttttat
    76621 acagcaaata atacaataat ggttcatatt agaaatttaa gaaaaaagtt agaaaaagac
    76681 caaaaaaatc cacaatatat aaaaaccgca tggggaaagg ggtattatat tgattaacaa
    76741 aattaagcca tctaagttgg gagttatact attgatatat gtagtaatct cagctgcagt
    76801 ggctttcatt ttaaatatac cattttactc tgctgttgaa tctatagtaa aagatgtatc
    76861 agaagggaaa gtatatttta aaagagaagt aaatcaactt atggataatt ttcaaactta
    76921 tgtgaataac aatggtataa gagcagatga tggagatgat atagcaaaat ggaatagtga
    76981 taattggttt gtttctatga agatatataa aggaaataag gttttttatg atacattata
    77041 ctatcctaat gaagtacctg ataagagtga ggaaaatgag ttttatagca gtcccatggc
    77101 tagaaactat gagatacaat tttctgatat taaagctaat atagttgtag tagctttatt
    77161 tagatttaga tttcaaaatt ttatagaagc aatttctatt acatttatgg cacttacatt
    77221 tattataaca tttttactgt tgtttagtag aaagataaag tacctaagga aaatagaaat
    77281 gggtataaag atagtagaga gtggcagttt aaagtataga ataccagtaa aaggtaatga
    77341 tgaattatca tcacttgcat ctagtattaa tgagatgagt catacattgg agaatcaact
    77401 tataaaagaa agggagatga agaataaaaa taaagatata gtaacttcaa tatcacatga
    77461 tgttagaact ccattaacgt cagtaatttg ttatttagat ttgattaaag accataaata
    77521 caatagtcct gagcaattgg aacaatacat aaaaaatgta aggtctaaag catatcaaat
    77581 aaaagattta acagatgacc tttttacgca ttttgttaat tctggagagg aagtttcaaa
    77641 agggtatgaa aaaataaatg ctaatgaatt tatttttcaa ttacttttag atagttcata
    77701 tacattggag gagaaaggat ttcatataac aatagaaaat gaactttata accaatatca
    77761 tatttatgta gatgttacac aatttagaag gatatttgat aatttatgtt ctaatataat
    77821 aaaatatgct aggaaagata agcctatagt atttttaatt aaagtagagg attctaaatt
    77881 actaattata caaaaaaata gagtaagtaa tttttctaag aatgtagaaa gttttggaat
    77941 tggtataaag aattgtgaga aaatagttgc atatcataat ggaagcataa atgtaaattc
    78001 agatgataaa gaatttgcta ttgaaatatc tattccagca aaagaatcat aaaaatttaa
    78061 attatatatt gtgtaattgc tattatatat tgtgtaattg cttatgtaaa attctatgaa
    78121 gcaggaagca tgtgacttta gttatgggat gttcaatatt gtgtacctct aggcaagtaa
    78181 caagtttact tacaagacca tataatatta ttatcttaaa aaaaggggac ataataatat
    78241 ggttttgaca ggagcacatt atatttacat tctatttatg gtaattatat taattactat
    78301 gattatgaaa aaggatacaa tagtaccatg tatattaggt gtttttttta tggggttgtt
    78361 ctttgaaaaa aacatttttg gtgctataac agcagtgttt aactctttta ttatatcttt
    78421 aaatgagttg ggtccaatta tcttgattat agctattatg gttgcattat ctaaagccct
    78481 agaagccaat aatgctatac agtatatggt gagaccattt tcgagagtta taaaaaattc
    78541 taatacagca ttttttgtaa ctggatttgt tatgttagtt ctttcttggt ttttctggcc
    78601 aactccagct gttgccttag ttggagctgt atttttacct gttgctatga gagctgggct
    78661 accagcaatt ggtgttgcag tagcattaaa tttatttggt catggtcttg ccttgtctac
    78721 agactttgtt attcaaggag ctccaagtat aacagctggt gcagcaggtg ttgcagtatc
    78781 atatgttata aatgatggta tgattttgtt ttgggtgatg ggaatcgtaa ccatatctat
    78841 ggcattttat acattaaaaa gagatattaa caaaggaatg tttagagaag aactaaaagg
    78901 ttttgaaagt gaagaagtag aagagttcaa tggaaaatct aaaatagcaa ctattttagt
    78961 agctctggga tttcttgctg atataattgc tatgtatata tttgacctaa aaggaggaga
    79021 tgcatcagct cttttgggtg gaacagcagt atttttaata ataattataa atgttataaa
    79081 ttttggcaaa gatagtcttg aaaatgtatg tgagaatata attgatggtt ttgtgtttgg
    79141 gataaaaata tttggagcaa ttataccaat agcagcattt ttctatatgg gtgaagttgc
    79201 accattgaca ggtgtatttg gaaaagtatt agcaccaggt tcacaaggtt tattatcaga
    79261 tataggcata gctctttctc aaacaatacc acttaataaa tttgccgtat caggtataga
    79321 aactgtagtg ggagcaataa ctgggcttga tggttctgga ttttctggga tttcattagt
    79381 tggttcactt gcctctgtat ttggaactgc tataaatgca agtgttggtg cactagctgc
    79441 attaggacaa atcagtggta tatgggtagg tggaggctgt ctagttcctt ggggtcttat
    79501 ttcagcagcg gcaatttgtg gagttagtcc aatagaattg gcaaagcgaa actttgtccc
    79561 agttataacc ggattggttg taacaaccat agtagcaatg tttattattt aataaatcga
    79621 ataaatcttt aattgtataa attaaaaata gatgtgtagc tataatttat tataattgtt
    79681 ttactaattc taagccatac atctattttt attgatattt ttattagtat tctttaataa
    79741 aataatattt ttcattatac tatacataac atctaaatat aacatctaaa atactgttat
    79801 ctatatttaa tatcttgcca aattttttga tgttttattg ccctgtccca ccaacatata
    79861 taagctatca caagttttta cctatactat caccaaacaa aaattctaat tttaatttat
    79921 tattttataa cctatattat attaactata ttagtttgta gactttagat gtaattataa
    79981 atattaatac tccaagagtt caaattcata tcctagtttt cttgcatttg atataaaatc
    80041 atttaaaatt tctgtactag ttttggaaac tgcatgtagt aacaagatag aaccatcatg
    80101 tagattatcc attatttttt gcgttgcttc ttcatgtgat ggttgtttat cagtatccca
    80161 gtctctgtat gcaaaactcc aaaatacagt cttgtatcct aaatttttag tcattgccaa
    80221 gcttttttct gagtattttc ccataggagg tctaaaaaat ttaaccatat ctttcccagt
    80281 tacatcttta tataactttt caacatcata taattcgtca ttaaagtttt ttagattgct
    80341 tgtttttgtt ggcatagatg gatgggtttt actatgattt ccaactatat gtccctcaga
    80401 aaccattctt tttactaaat ctttattttc ttttatatat ggagctgtaa caaaaaaaac
    80461 tgctttaacc tgattttgtt ttagagtgtc gagtattttt gtagtgtatc cattttcata
    80521 accttcatca aatgtaagat atagtgtttt ttgaccatct tttgtaggtc cattgtatag
    80581 ggcatcatag tcagaaaatt taaattcaat gtccgtattt acttctggtg ttttatgctt
    80641 attgtttggt atataaaacc aatcaagagt agtgttatct aaagaagata catctaattt
    80701 attattatca ttattagtag tggcttttga tggtttatct gttccgtctg aaactattac
    80761 attggaagta tctttaccac tatctatttt ttccttatct tcttgtaact gtgtttcctt
    80821 attttgattt tcgttttgat tgttttgtga atttgaacat ccaacaataa aaaaaataga
    80881 aatagaaagt agtgttaaat aatgcaatac ttttctaaac attttgaagc tcctttctaa
    80941 attaataata attgcatata ataataatta tatgattaat tacaattgca tatagtatta
    81001 ttataattgg aaattactat taataatagt tatatcttta aaaataaggt cacaatagta
    81061 tgacaaaggg tgaataatca ataaatttaa aaaaaaatga aaaaaatagt tgaccatatt
    81121 atgagaagat gttattataa taaatgtgct caacaagaag taacaaaata acaagcacaa
    81181 agaagttaat tgaat
//
LOCUS       DAMJVK010000006        71853 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.006, whole genome shotgun sequence.
ACCESSION   DAMJVK010000006 DAMJVK010000000
VERSION     DAMJVK010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 71853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71853
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.006"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(<1..1006)
                     /locus_tag="PC414_000413"
     CDS             complement(<1..1006)
                     /locus_tag="PC414_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2462828.1"
                     /translation="MAVVEKAYKFRMYPNKKQQELINKTFGCCRFVYNKYLAKRIEVY
                     KNNKETFTYKQCSSDLTNLKKELKWLKEPDKFSLQNALKDLDNAYKKFFKEKAGFPKF
                     KSKKINRFSYKTNFTNGNIMYCGQYIKLPKLGMVKVRDKQVPQGRILNATISKEPSGR
                     YYVSLCCTNVDIEVFENTNNQIGLDLGIKEFCISSCGDFIENPKYLKKSLNKLAKLQR
                     ELSRKTIGSLNRNKARLKVARLQEHIANQRNDFLQKLSTKLIKENDIICIEDLQVKNM
                     IRNRKLSRLISDVSWSEFVRQLKYKANWHGRQIVKVGKFFASSQICNKCGYKNEEMKD
                     L"
     gene            complement(1008..1415)
                     /gene="tnpA"
                     /locus_tag="PC414_000414"
     CDS             complement(1008..1415)
                     /gene="tnpA"
                     /locus_tag="PC414_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HDF2462829.1"
                     /translation="MEVTHGRGYVYLVQYHIVWCVKYRHPVLTNEIEEDLINILNKIA
                     EDNKFQILECNSDKDHIHLLINCSPQHYIPDIIKALKGVSARLLMKKHGEQLKKKLWG
                     GHLWNPSYFVATVSENTEEQIRKYINNQKKQVK"
     gene            complement(1984..2193)
                     /locus_tag="PC414_000415"
                     /pseudo
     CDS             complement(1984..2193)
                     /locus_tag="PC414_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861712.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4004 family protein"
     gene            complement(2295..2843)
                     /locus_tag="PC414_000416"
                     /pseudo
     CDS             complement(2295..2843)
                     /locus_tag="PC414_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426706.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3202..5112)
                     /gene="phnW"
                     /locus_tag="PC414_000417"
     CDS             complement(3202..5112)
                     /gene="phnW"
                     /locus_tag="PC414_000417"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HDF2462830.1"
                     /translation="MKKIYGEKIKAVVFDWAGTTVDYGCFAPLNVFIEIFKRRGIDVT
                     MEEARKPMGKLKIDHIREMCEMDRIKNIWSDKFGKVPTEDDVNELYAEFEPMLFETLE
                     EYTTPIPHVVETIEKLRKNGLKIGSTTGYTREMMNIVEPNAAKKGYSPDFLVTPSEVS
                     QGRPYPWMCYKNAEALGVSPMSSMVKVGDTISDVKEGVNAGMWSVAVIKGSSELGLTQ
                     EEVENMDKEELKAKMSIVSKKFKEAGAHFVIETMAELEDILIKIENETIKSDFVPEND
                     YILLTPGPLSTTKSVRASMLKDWCTWDVEYNNLVQDVRRRLVSLATQNTDKYTSVLMQ
                     GSGTFSVEAIIGSTISKDGKLLVIANGAYGKRMKDICNYLNIQFVDCTFKDIEAVDLN
                     VVENLLKENKDITHISMVHCETTTGRLNPIQEVGKLAKEYNKIYIVDAMSSFGGIEID
                     VEDFNIDFLVSSSNKCIQGVPGFGFIIANKEKLSKCKGIAKSLSLDVYAQWETMEKNN
                     GKWRFTSPTHVVRAFYQALLELEEEGSVEKRYARYKENQFTIASRLKSLGFDTLVNDN
                     AQSPVITTFLYPKNAKFEFMEFYTYLKDNGFVIYPGKLTDIDTFRIGSIGEVYPTDME
                     RLADVIEKFINR"
     gene            complement(5391..6176)
                     /locus_tag="PC414_000418"
     CDS             complement(5391..6176)
                     /locus_tag="PC414_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDF2462831.1"
                     /translation="MSLNSIDRKKEILKLLDSKGKVNTKELVKRLEVSSETIRRYLEE
                     LENENKLKKVYGGAIKINYGVIEPEHIKRNNTNLDKKQSIAKYAAKFVNDNESIIIDE
                     GTTNLQIVDYIISVNNLKIITNSYPVLSKLISYENQGLFDGEVIFIGGKVNLKHQRTA
                     CPMSIDTMKNLYVDKAFISSEGILDSFGISSIDQNKALLSKEYIKNSKESFVLCDSSK
                     IGIASMYKIADLEELDFVICDVEPPNEWKDKLNEINITWIEAK"
     gene            complement(6393..6623)
                     /gene="dltC"
                     /locus_tag="PC414_000419"
     CDS             complement(6393..6623)
                     /gene="dltC"
                     /locus_tag="PC414_000419"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HDF2462832.1"
                     /translation="MQETVIEIFEDVLGTDEIREDLDLNLFETELLDSLAIIEVLLEI
                     ENRLGIELQPTDLERKDMSTVNNLVKFLETRK"
     gene            complement(6642..7790)
                     /gene="dltB"
                     /locus_tag="PC414_000420"
     CDS             complement(6642..7790)
                     /gene="dltB"
                     /locus_tag="PC414_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HDF2462833.1"
                     /translation="MIFSQYGDYFYLYILLLTSIPAVILGLMGKNIKYYGMLASLFMI
                     FLIVGIDVQLKYLVIFIILEVIIVKGYEYVRRKTKNKYIYWGFLFASMLPIIINKISP
                     VTSFGIIGFIGISYLNFRTIQMVIEIYDGAIKEVKISKMLYFMLFFPTLSSGPIDRSR
                     RFEQDLEKQISRKEYIEEYLLPGIKNIVMGVGYKFVIAFLINTYWVSRIPKDMSFINI
                     LSYMYAYSLYLFFDFAGYSLFAIGTGYIFGIQVPINFDKPFISKDMKEFWTRWHISLS
                     RWFGDYIFSRFVMSSMRKKRFKKRTTAAHVAQMITMITMGFWHGLTWYYVAYGVYQGL
                     ALVLTDIYQRKSKFYKKHKKDKWFERVQIFITFHIVCFGLLIFSGYLA"
     gene            complement(7790..9304)
                     /locus_tag="PC414_000421"
     CDS             complement(7790..9304)
                     /locus_tag="PC414_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase"
                     /protein_id="HDF2462834.1"
                     /translation="MKIIEGIKKYSNTDRTALMCNGDKLSYKDLNEYSDAISVFLKDV
                     YKEEDTPIVIYGNKENMIMACMIGALKSGRAYVPLDISFPIDRVFEVTKEIKPKVLFN
                     FSDEKDFGDINVIDMDKLNYIINEYQGKSLDKENWVKDDENAYILFTSGSTGKPKGVQ
                     ISSNNLDSFSDWISPYLNIDGSEKVIMNQPAYSFDLSVTTIYPGLIHGATLFSISKDV
                     LADYKELFRQFSISDIAVWVSTPSFAGVCITEKEFNSKMLPNLESMIFIGEALSKNLT
                     KELMSRFPNTRIINGYGPTEATVGVSVNDMTQKAIDDEKSLPVGYPMSNCKIKILDED
                     GNELKENEKGEIIIIGPSVSKGYFNNKEKTDEVFFYDEIDGVKWRAYKTGDMGYLLDG
                     NIYYCGRKDFQIKLNGFRIEIEDIENNLRKVHNVKNAVVLPVYKDEKIAYLKGIVELN
                     EKNDLSNIKNGMIIKKELGKYIPSYMIPRNISIISEFPTNINGKIDRKKLMEEI"
     gene            complement(9425..10615)
                     /gene="dltD"
                     /locus_tag="PC414_000422"
     CDS             complement(9425..10615)
                     /gene="dltD"
                     /locus_tag="PC414_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HDF2462835.1"
                     /translation="MRKLIYFITPFIIGVVFLFGLDKFLDSKTDELLREKNLLPIMDD
                     TLSDIKDKGVTANNHFLREKDIMILGSSELSNSTKQHPKYYFNTNRSKNKVFAIGRAY
                     TQTLQDAAILGSMNPNIDNKKVVLLISMQWFMEKDGVTSHHYQSRFSPIQFYRFLDNP
                     KISKQNKIEYAKKSSKLLWGSDEYKAEALYAKLYEPKTFPEKAEKVLLEPYFQGRKYC
                     IALKEKGILYKRLIKLDKKRATKRKRPINWSYERKKAIEDAKKRVGKNPLNIDKYYYK
                     EHFKDGIEQYKGRDKDVNLLTSKEFENYKLMLNVCTDLGIKPVVVLIPSMDKFYNLTG
                     ISEKERNQYYDKAQKIAESKGFEVLNLKDKGSDKYYLRDVMHLGTKGWVDVCERLFKI
                     FKEQ"
     gene            complement(10903..11922)
                     /locus_tag="PC414_000423"
     CDS             complement(10903..11922)
                     /locus_tag="PC414_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDF2462836.1"
                     /translation="MMGKIRFGIVGTSNIANIFLRAASRVKDFELVAVYSRNLEKARE
                     FGTLYGASIFFDDLEQMAKSKDIDAVYIASPNALHSKQTITCLKHKKHVLCEKSLASN
                     LKEVKSMIKTAKDNNVLLMEAMRITCVPNFKAVKENLYKIGKIRRFFGSYCQYSSRYD
                     KYKNGIIENAFKKELSNGALMDIGVYCIHPMVNLFGAPKTVKAVSHILQTGVDGEGSA
                     VFQYDDMDAIIQYSKVADSYIPSEIQGEEGSIIIEKLNLFEKAIIKYRDGREEDISIP
                     KEKAEEKPREIEGMYYELVEFISLINNNKIESNINSHENSIIVMEIMDEIRRQSSIVY
                     PADSI"
     gene            complement(12098..12637)
                     /locus_tag="PC414_000424"
     CDS             complement(12098..12637)
                     /locus_tag="PC414_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HDF2462837.1"
                     /translation="MDNVGSLLYFLFNFFLYGFIGWIIENLYSYYKQGHFQEDGFLNG
                     PFKPMYAIAMSIIIEVSRFIDPNNIFALLLLCLVVPTVVEYITGILMKVYFNKVYWDY
                     SDYKYNSRGIVCLKFSVYWTILTFIGVRYFQVYIVDSLYSLIIPYWPIVVTILLVTLF
                     VDEILTIKFLRGNKDIVER"
     gene            complement(12738..13418)
                     /locus_tag="PC414_000425"
     CDS             complement(12738..13418)
                     /locus_tag="PC414_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDF2462838.1"
                     /translation="MQKVEGIIFDMDGVLFDSERISLEFWMETFEKYGYTMTKEIYTS
                     VMGRNRKGIIEGLTDIYDSSVPIIDLYDEKTKNMIEFMERKGAPIKLGVNELISFLKE
                     NGYKMAVATSTKRERAVKRLAKANLKDYFDAIVCGDDVVNSKPNPEIFLKAAKKINVN
                     PQNCIVIEDSPMGVEAAYNGGIRCINVPDLKEPDEQIKSQSHKILENLLEVREYLKSL
                     NSKECHNL"
     gene            complement(13447..14736)
                     /locus_tag="PC414_000426"
     CDS             complement(13447..14736)
                     /locus_tag="PC414_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDF2462839.1"
                     /translation="MRELLKVTLIVMLVLINAFFVACEFAMVKLRSSRIDTMITEGNN
                     NAKRCKIIKDNLNSYLSACQLGITLCSLALGWMGESTVKELILPIVSVFNLSESIIYT
                     ISISISFLIITMMEVVIGELVPKALALYNTEKIMLSTSFLLVGFYKLTYPIIYFFNLS
                     TDLFLKPFGYSQADEVDEPHTGDEIRLLVEESYKSGLIDESEQRLVDNIFEFEEKKIR
                     EIMVPRTDMVCIYESDSEEKILAILKEEGVTRYPVCRKNKDDILGFVHIRDLYNQKIN
                     ENKIELEEILRDIIYISENLTIDKALERIRKEKLQLAIVVDEYGGTSGVVTIEDILEE
                     IVGEIQDEFDKEETHIKKIGAGSYLVDGTESINYINKYFGLDIEHDGFDSIGGWISYM
                     LGSNIKVNQSVTFENYNFTILELDKLRVVKLIIKSVI"
     gene            complement(15185..16786)
                     /locus_tag="PC414_000427"
     CDS             complement(15185..16786)
                     /locus_tag="PC414_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDF2462840.1"
                     /translation="MIYDLILKNGFIIDGTGNPGFYGDIAIKDNLIAKIDSKINSNTN
                     KEIDCYGKIITPGFIDPHVHEEIVAILDGKFEKFLKQGVTTTINGNCGHSITPYSSEN
                     VYEYMYKNGLLLEEEKKYLIDKNKCWNNFTEYCDLISKSGISVNMGFLLGHGTIRWSV
                     MGGSKDRPPTEKEKNEITDIINDGMKSGAFGISTGLAYIPSKYADIDELVDIARQIKE
                     HDGIYTSHIRDYIGRYNAVKEAIEVGQKSGARVQVSHLSPVEIEAFDEILKARYNGVE
                     IMVDTVPRSSGHCMKKKRVIQFIMAISSSLFELGIDGVMDALRNEEGRTLILKEAFIL
                     GDRGSIILLNTKDINMEKKSIREIATQKGIDEDKLLLDLLLDGDEELIFCLGGMYRAD
                     FPDKLHDDKIIDNPFVMVGSDCLFSVGGDMSWFELQRRGAFPIFFNMYRKSGVRLEEI
                     VRRVTSLPARQFKIKNRGILKEGLIADIAVIDMDNYSYPRSEDIDFSKPQLLANGVEY
                     VIVNGKIALENGEITENKCGEVLKR"
     gene            complement(16897..17574)
                     /locus_tag="PC414_000428"
     CDS             complement(16897..17574)
                     /locus_tag="PC414_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2462841.1"
                     /translation="MSKVKSIYNEEYLPFMIRYGRLTLSLGIIAALVPGIILSFGFGI
                     MPPISALLASTMAIVSMSAPNYIIEPVSYSPILGIPGTYMSFLSGNISNMRLPCSIAA
                     QKAAEVESGTEEGSIISTIGIAVSILVNISILTIGVILGGSVLSKIPAEVVEKLNLIL
                     PALFGSVFGQVFLQDKKLGLVAIVISVLTIILSKQGIIPQSLVVLICVFGTILIARAM
                     YKDKLSD"
     gene            complement(17587..18282)
                     /locus_tag="PC414_000429"
     CDS             complement(17587..18282)
                     /locus_tag="PC414_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HDF2462842.1"
                     /translation="MDYLKLANHPLLWIASTVAVSIVVVQSLIFAIKSCKVGKTMGIT
                     DKQIKSAVKSSAISAIGPSMTIFAGMVSLIVTMGGPIAWMRLSFIGSVIFESMSAGFG
                     TDALGITLGSPEMTKLAFTNAVWTMILGSLGWIIFTLLFGHKLDKITNVISSGKKSFI
                     PIISLGAMLGSFAYLNADRVLRFDNGTIACISGMAIMVILCMLEKKKNIKWLREWGLT
                     ISMFSGMIIASVI"
     gene            complement(18334..19533)
                     /locus_tag="PC414_000430"
     CDS             complement(18334..19533)
                     /locus_tag="PC414_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDF2462843.1"
                     /translation="MKSAYKGYEAYSYLEKGKDISSIEMCKGDKRVKEYLIELDDIQE
                     AKVKSIVDEKIFISLHEHPVLFPEHLERDIFEYNREGKQRCAYEALSRGYYDAIFDNL
                     MDGVCTITSNSGWKWDDILIDLGMRLCDLAHQDFVIKCEKVEDIYRAKSEGKVALIPT
                     LEGAAPIENELDRIDILYGFGIRLMGITYSESNALGSGLKEEKDGGLTNFGKKAVERM
                     NKIGMAIDCSHVGVQTTLDVIEYSKDPIILSHVGARSLWNTKRLTPDEVLIACAKKGG
                     VIGVEAAPHTTITEKNKEHSIESFMEHFEYIKNLVGIDHVAFGPDTVYGDHVKLHSVL
                     AAKFDIKKITGTKEHPKVEYVKGLENPTETSKNIVRYLVKHGYSDEEIEKVLGGNILR
                     VLKQVWK"
     gene            complement(19794..21839)
                     /locus_tag="PC414_000431"
     CDS             complement(19794..21839)
                     /locus_tag="PC414_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2462844.1"
                     /translation="MKKHNILFVSTDDKINIDISKQLENIFGEFCNIDNLVYVNRINI
                     ELSSYELVVCSDNDIKEYIHNNIDKNIPIVIVHRTINIENINQIISIENDSDVMVIDA
                     YKESADETAKIIRKLGLIHINLIPYYPGCDKSKCEIGIITGSRNSIPQNIKQIIDIGD
                     KVIDINTVIEIFTKLNISIDKLHIIKEKYNEDTVSGYRYYTTMNKTMKSFLEIIDEGI
                     ASVDKLGKFIYCNKVFSNLVGIDQNEIISNNFMDLFSDKVVKKIFFQEDEVNDEVVNL
                     NNKKLIINKVNVYENNERIKSIISIKNISAIQVLEDKIQNKLQSKGFVSKYTFESVVG
                     ESKIIKEKINIARKIATTDFSVLILGENGTGKEIFAQAIHNESLRKNKPFVAVNLSSL
                     SDTLIESELFGYDEGSFTGAIKGGKMGIFERAHTGTIFLDEIGDISLDVQQRLLRVLQ
                     EKEVMRMGGSKIIPIDVRIIAATNKDLKKKIKEGSFREDLYYRINVLHIEIPRLRERK
                     EDIPLISKYFLDEINSNKCFTEESMQALTLYEWPGNVRELKNLIYYIDTIVEEDRVDY
                     EHLPEQFRFEKNNTLVNENFDSIILDFKQSNFFEESICILTSIETWNNKNILLGRNKL
                     QEILKEKDIVLSVDQIRKRIDKLKSHGLLLSGVKKQGSFITDEGKNFISYVKIKGVI"
     gene            complement(22250..23059)
                     /locus_tag="PC414_000432"
     CDS             complement(22250..23059)
                     /locus_tag="PC414_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2462845.1"
                     /translation="MYYVYVNDINIAVYDLNPSAKKTVLFIHGWPLGHKIFEYQTNIL
                     PKLGYRTVSIDLRGFGKSDATSGGYTYSQLADDIYKVVHAIGLKDFTLVGFSMGGAIV
                     LRYMSLFNGYGVSKLVLAAAAAPSFVQRPPEFPYGMTREDVNKLIAQACTDRPEMVTD
                     FGEKVFASNPPESFRRWFNDIGFSASGIGTIGTAVSLRDEELFNDLKCVRVPTGIFHG
                     KLDEICPYEFAVFMNEKIEDSILYTFEYSGHAIFYDELKLFNQEFLQFLEE"
     gene            complement(23143..23676)
                     /locus_tag="PC414_000433"
     CDS             complement(23143..23676)
                     /locus_tag="PC414_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HDF2462846.1"
                     /translation="MSGAHYDMHKRNGYSSDEPYPEIKVLGPNKYYAELLMDDYAGVS
                     SEFTSVNQYLYHNFDLDETHRELSEMWINISITEMLHMEILAKTIRLLGGNPVYRGST
                     SSCGAYWNGGFVCYGNSICNRLKLDLHLEHVAINNYYKDISLIEDPYIKAILNRIILD
                     EKLHVSLFEKAIEKYCK"
     gene            complement(23826..25175)
                     /locus_tag="PC414_000434"
     CDS             complement(23826..25175)
                     /locus_tag="PC414_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HDF2462847.1"
                     /translation="MELKNFKDILFKKALSEGFEECEIYYYTGENLSINIYEGEVEKY
                     NLDKSFGLSFRGKVNGKIGYSYTEILDDKAVDMLIKNVKDGVNTIENEDVQFIYEGDK
                     HYNDVKTYSKELENLEADKLIDLALEMERETKAYSDKVVNLSKCTISYSVSSNGISNT
                     KGLNLSNKTNMLIGFVVPIIEDNGQKYDGIGYNIANSIEEIKPCEIAKLGVDNALSKV
                     GGKSITSGKYKTILLNEAMVSLLSTFSGIFNADSAQKGLSLLKNREGDMIASPIVTIV
                     DDPLLENGMASTPFDDEGVATFKKEVVLNGKLITLLHNLKTANKAGVKTTGNGIKSSY
                     SSPISIYPTNFYIEKGDKSLDEIIKDIDEGLMVTSFAGLHSGANSVTGDFSLAAKGFY
                     IKEGKKVFPVEQITVAGNYFDLLKDIEAIGEDLEFPMSSIGSPSVVIKELSVAGKDE"
     gene            complement(25187..26569)
                     /locus_tag="PC414_000435"
     CDS             complement(25187..26569)
                     /locus_tag="PC414_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HDF2462848.1"
                     /translation="MLSKNIASKVLEKCLITGGDFAEIFEEDTINNSISLIDNKVENA
                     IGGRNYGIGIRIFKGLKSIYAYTSDNSLNSLLDVAYKAAVALGKLEDGKSVILNDSIK
                     INNIHNIKIYPNSIGNKDKVSVMKVAYKSAKEFSNDISQVSVSYLDKDQKILIANTEG
                     IYVEDRRIRTRLGISSIASKGNENQTGFEGPGGCKGFEIFEEIDPEYYAKESARVAHT
                     MLHAKNCPAGNMMVAIDNGFGGVIFHEACGHSLEATSVAKGNSVFADKLGQQIASTKI
                     TAIDDGTIPNYWGSMNIDDEGNPTQKNILIESGILKSYMIDKLNGRRMGMNPTGSSRR
                     QNYKFAPTSRMTNTYIAAGEDRPEDIIKSIPDGLYAKKMGGGSVNPVTGEFNFAVSEG
                     YLIKNGEIQEPVRGASLIGKGSDILMNIDMVGNNVKQAQGMCGSSSGSIPTNVGQPMI
                     RVKEITVGGR"
     gene            26813..28027
                     /locus_tag="PC414_000436"
     CDS             26813..28027
                     /locus_tag="PC414_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDF2462849.1"
                     /translation="MNQEYVKGNPIFTNINKVPRQFPYLTDDIDTDVIIVGGGVTGCI
                     CAYYLAKNNIKSVILEKGRIAHGSTSVTTSLLQYELDDNLIDLTEVMTLKDALKAYNL
                     CTSALEELDTFIELYGNKCDYAKRDTLLYTANKLEVKAIKEEYNLRKENGFDVEYIDE
                     STNPFSFDLKSGLIAKNGGRELDPYKYSHHLIDVSLKNGLQVYENTEVKKVDLSNDKV
                     TAEVSYGYKVHGKKLIVATGYNTSLFTKRNFATKSNTFNIATKPLKNIASWKNNILIR
                     DNCDPYNYLRTTKDNRLIIGGEDVSFDDIENETLANEKYDILEQRLKSMFKDIKDIEV
                     EYKYCGCFASTLDNLGFIGPDNKNNNLWYCLGYGANGILFAILGGIMLSELYLGKQNK
                     NMKLFKVDRFDK"
     gene            28204..30117
                     /locus_tag="PC414_000437"
     CDS             28204..30117
                     /locus_tag="PC414_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2462850.1"
                     /translation="MKYGGIIMKEILVIAPTKGTYEKSIHIVKKNKYTNIDVVFGNLK
                     EGIPLAEKSINHGTRIIISRGGTYNMLKATYNIPIVEIKVDAYDIIKSYKEVKNSNEP
                     FGIIGFNNVIYGFDIIEEILNKKITMIEIEKEEEIYDAIEKYRKKGINTYIGDTTVAH
                     IVKRLNCKGILIESREENILRAIQQAEQILEATKDEQKRRLQIEAMTDFVHDGIITVD
                     KNFKITLFNKSAEKIFGIKKENALYHNVVDVIPNTVLPQVVETMEPQIGEIQNIGSTK
                     IITNRVPIIVDGQIQGAVSTFQDSKEISSFEHTIRRSLLSKGLSAKYTFDDIIHASEK
                     TKDCIELAKKYACYDTPMIITGESGVGKELFCQSIHNYSKRKNAPFVAVNCAAIPPSL
                     IESEFFGYEEGSFTGARKKGKAGVFELAHEGTIFLDEISEIPLELQGRLLRVLQEKQV
                     MRIGGDKVIPIDVKIICASNKDLKNMMRNGLFRQDLYFRINILTLYLPSLKERKEDII
                     KLSEFFIHKYSSKYNKTPLIITPNIKKALLEREYEGNIRELEGMMERAVIVESFDSIL
                     LEKLSNEYTNNIVDKDNSDFECSNLDLKTLEKKYIYKIYNENNRNTTKTCEILKITRS
                     TLWRKLKEIEDDV"
     gene            30388..31362
                     /locus_tag="PC414_000438"
     CDS             30388..31362
                     /locus_tag="PC414_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HDF2462851.1"
                     /translation="MHILKSVKKIPGGLMVCPLLLGALFNTVCPQALEIGGFTTSLFK
                     TGAMSILALFCLCNGAQINIRQAGKPLTKGIVLTATKFIIGAVLGIIVSKFIGPKGII
                     GITPLAIVATMTNSNGGLFAALAGEYGDSTDVGAISILSLNDGPFFTMLAFGVTGLAN
                     VPIVALFAALIPILLGFILGNLDEDIREFLAPGTTLLIPFFAFPLGAALNFNQIITAG
                     LPGVVLGLGVTIITGMGGYFVMKLIRAEHPAVGAATGTTAGNAAGTPEALAAIDPTLA
                     TIAAVSTVQVAAAIIVTAICCPMLVSYLDKREKKKKSIFKTNKNIEVN"
     gene            31383..31658
                     /locus_tag="PC414_000439"
     CDS             31383..31658
                     /locus_tag="PC414_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HDF2462852.1"
                     /translation="MNAIIHANEKDNLVTCVRPLIKGEKVSIDGKSYIVNDNIPVFHK
                     MATEDVKKGDVVYKYGEIIGIATVDIKTGDYVHVHNIESTRGRGDKK"
     gene            31671..32834
                     /locus_tag="PC414_000440"
     CDS             31671..32834
                     /locus_tag="PC414_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HDF2462853.1"
                     /translation="MKLMGYLRENGQFGIRNHVLIIPTSVCSSETATRIASLVPGAIA
                     IPHQHGCCQIGSDIELTAKTLIGFGKNPNVAAVLVVGLGCDGIQAKELASEIATTGKK
                     VNYVVIQECGGTLKTVSKGAEIVSKMAREVSKEVRVEFGMSEITLALECGGSDPTSGI
                     ASNPSIGVASNLLVDEGGSSILSETTEVIGAEHLLATRFEDEEMKDKFLKFVSDVEKR
                     AIAMGEDLRSGQPTPGNKAGGLSTIEEKSLGCMYKAGNKPFKGALEYADIVPPDKKGL
                     YFMDTPGQDIDSITGMVAGGAQIVIFSTGRGTPTGSPISPVIKITGNSDTYNKMPDNI
                     DINAGRIITDGAKIADIGQEIFNEIIEVCNGKHTKAESLGHREFGIYRISSTF"
     gene            33171..35450
                     /locus_tag="PC414_000441"
     CDS             33171..35450
                     /locus_tag="PC414_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2462854.1"
                     /translation="MNFENTKNKKIIATLLLTLLLIVSLTCVTFIMNLNKVTHNETER
                     YLVEVSQSISTTINVKCKSIFRTLYSMGDTYFEINDSEKEVNRYLSKKLKLFEFDWLG
                     IIDSNGNFVSALKDTGNVKGQDFYRKALQGKETITPVKSQSGTYGILYSVPLYNGDKI
                     VGAVAAWNTIDTMRNLLSVESFHGEGFSQIIDQDGNFIVSSANKNAPKNVVNFFDMIK
                     HQGKQVDKNKLEEMKINLKKNKTGSMYYTLNDGISKAMHYLPLQEENLYLLSIVPTKT
                     ASAQFDLLLKKAIFINILIVILFLLLIILIMFINQKNRAQLIKIAYVDSVTKGYSKAR
                     FDIEAKKIIQSSSSESYSLVSINIQKFKLINDSFGSEAGDKTLKYIHDVISNYLEPGE
                     LLCRVSNDQFNVLIKTIPQKEILKYIENITNDINSFNKNEECKYFIALSIGVYKIDDP
                     NLPLVSIQDRANVARKNNKGNIGNHPLYTCVFYSDLERLKMIKEKEMENRMEEALNNN
                     EFIVYLQPKISLKDNTIVGAEALVRWQDPNKGLIPPDEFIPFFEKNGFITKLDHYVFE
                     KVCSMIRKWIDNGETPIPVSVNFSRANLNNIDFLEKYRMLRNKYNIPAKLLEIELTET
                     LVFENLQKLINIIDQIHEEGFQCSLDDFGSGYSSLNMLKEIKVDTLKLDRAFFSSPNA
                     DNIAENHVIESIVNLAKKLNMNSISEGVETILQMDYLKKINCDMIQGYVFSKPLPQEI
                     FEKMTFGKKLEETSMDSSV"
     gene            complement(35545..36885)
                     /locus_tag="PC414_000442"
     CDS             complement(35545..36885)
                     /locus_tag="PC414_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2462855.1"
                     /translation="MENQEALRHEKIWILLFRYSIPAIIAMMVTSLYNVVDRAFIGSM
                     EGIGSIAIAGLGVTMPVFTLIIAFGMLVSVGASTRLSIKLGERNREEAEKILGNALTL
                     SIIISLIITILGLVFLEDILFILGASKDSIFYAKDYMSVILVGSIFNLVAFSLNNAIR
                     AEGNPKLAARTMIVGCVLNLILDPIFIFVFNLGIKGAAIATVLCQIVVFFWVIHYFIR
                     GKSNLKLKKCNLKLNKDIVKKVFAIGMTPFAMEVAISITHMFTNNSLKVYGGDLAIGA
                     MTALTSILLMFMMPVFGLNQGMQTIISYNYGAKQYERAKKTLILSIIVSVVILSFGFL
                     VVQVFPEVFVGIFNKDSDLMDIAVRGISLNLITLPIMGISIVGPVYFQCISKVKHSMF
                     LTLLRQFILFIPLIIVLPIQFNLDGVWLAQPVADFIAMIIVLLFLKREFKTSRV"
     gene            complement(36897..37679)
                     /locus_tag="PC414_000443"
     CDS             complement(36897..37679)
                     /locus_tag="PC414_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2462856.1"
                     /translation="MNCINTKNLNVSYGNTDIIKNLNLNIPKGKITTIIGANGCGKST
                     ILKTIGRIISPNSGKIYINGEDIYKKKSKEIAKDMAVLPQSPQAPSGLTVSELISYGR
                     FPHKSGFGNSSKEDREIVNWSLEVTGIAEFKDRNMEDLSGGQRQRAWIAMALAQETDI
                     LLLDEPTTYLDLAHQLEILKLLDELNKKQGRTIVMVIHELNNAARFADHMIGIKKGKI
                     VCEGDAYDVMTKDNLKELFNIDAEIATDPRTNKPICITYDMV"
     gene            complement(37694..38716)
                     /locus_tag="PC414_000444"
     CDS             complement(37694..38716)
                     /locus_tag="PC414_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDF2462857.1"
                     /translation="MKNIIKINSKTLLVISILMVLIFITFLLSLNMGSFSIEPMDVIK
                     TFLGQGQRNHEIAIFKLRLPRIVIALLVGSALSTAGVILQGVTKNDLADSGILGINSG
                     AALFVVVYIYLMNGNVYEGMSNLTVFTMPIVALIGALFGAFLIYSLAWKGGINSSRLL
                     LIGIGVNIAFTSILTIFQLKFTTQEFNRVMAWTSGSIWGTSWKYVIAVLPFIVIFMGI
                     TIYKSKYIDVLNLGDEVSISLGVDVEKQRRRLIIYAVILSGVSTAVAGSISFLGLIAP
                     HIGRKIIGPKHKRLIPVSALIGTLLLLIADTISRNLLAPIEIPVGIVVSIIGVPYFIY
                     LMLVND"
     gene            complement(38722..39735)
                     /locus_tag="PC414_000445"
     CDS             complement(38722..39735)
                     /locus_tag="PC414_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDF2462858.1"
                     /translation="MTKLSKKNITYLLILVSIVVLVFGIVLSITIGAKDMNLSTVIDS
                     LIKMEDGINMRIVKDVRLPRAIAAALVGGFLAVSGAIMQGITRNPIAEPSVIGITQGA
                     TFAISISLVLQKKLPQLIHGSFSVMMFAFIGASISGLFIYFISSKSRGRVNNVKLALA
                     GVALGTLLISLASAISMYFNLSQQLSFWISGGLVGVKWEGIKLLFVAGGIGFILAIIM
                     APRITILSLGEEVAIGLGQKTNFVRFICIVLVILMTGASVSVSGNIIFIGLIVPQIAK
                     GIVGSDYKYIIPSSLVLGAVLLVYTDILSRMINPPYETPVGSITALIGVPIFIYLVRK
                     GEK"
     gene            complement(39863..40801)
                     /locus_tag="PC414_000446"
     CDS             complement(39863..40801)
                     /locus_tag="PC414_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2462859.1"
                     /translation="MKKIKSLAIFISIITTLVLVTACSDKNTEDKDKSETRVVQSVKG
                     EVKIPSNPKKIVDISGSSEELLLAGYKPVATANVDSYETDKLPSYIREELKGVKIVGH
                     SMMDTMDMEAILEVNPDLIIMSQRQEKIYDQLKEIAPVVMMKDYANDWRSKLTDVSKL
                     FDKEEEAKSWLQKYDEKATKLGKEVIEKNGEKTYLPVLASSGQFMVFSDGGIGTLIND
                     DMKLAKPKNMPKQDGITLPMVSMEGLTDIDADHIVVIATEADKKDLENSAIWAQIRAV
                     KDGNVTILDAAPFFSQSYNPIGKELLLESVKNELTK"
     gene            41071..41535
                     /locus_tag="PC414_000447"
     CDS             41071..41535
                     /locus_tag="PC414_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2462860.1"
                     /translation="MVHLVYCDNAGKKGEKVLDKILAGTKTMVIRGAAGRKIPHSRVF
                     EDELLYFMEKGTAKISAKATVKKVENYVKLTEDEISKILNENQPKLNLSEKQKVRWHK
                     KCLCLVEFENVEEITPLDFDHQGNMDDWLIVEKIEDVVVGTSIPYNYEKSKF"
     gene            complement(41647..41964)
                     /locus_tag="PC414_000448"
     CDS             complement(41647..41964)
                     /locus_tag="PC414_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF2462861.1"
                     /translation="MDEKMIEISFGIIGFAGDAKGLAFEAIKEAKSGNIDKARECIEK
                     SKESIHKAHKFQTELIQNEANGNKTEISVILIHAQDHLMTAMNFQQLAEEFIDVYERL
                     ESK"
     gene            complement(41977..42708)
                     /gene="chbG"
                     /locus_tag="PC414_000449"
     CDS             complement(41977..42708)
                     /gene="chbG"
                     /locus_tag="PC414_000449"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HDF2462862.1"
                     /translation="MTKIIINADDFGYCEAVNYGIISAHNNGIVRSTSMMANMPGVEH
                     GVGLLKENKTLNCGVHMTLSCGRPLLSNLKTIVDKDGFFIRRITDEIIEKMDCDEIYR
                     ELCAQIDRVKGLGIDISHLDSHHHIHTLLSLKPVIEKIVTKYNLPIRGGFEYNLEYSK
                     VVPLIDSFYKENVSEEYFIKNIDEIMKYDVVDIMSHPAFLDDYILNSTSYAIDRTKEH
                     KILTSKKVKEFLEKNGLVISSYRDI"
     gene            complement(42734..44044)
                     /locus_tag="PC414_000450"
     CDS             complement(42734..44044)
                     /locus_tag="PC414_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDF2462863.1"
                     /translation="MKKGLKIVTIGGGSSYTPELVEGFINRYEELPVKELWLVDIEAG
                     KEKLEIVGNLAKRMVKKAGVDMKINLTLDRREALRDADFVTTQLRVGLLDARIKDETI
                     PLSHGVMGQETNGAGGLFKALRTIPVIFDIIKDVEELCPNAWMVNFTNPTGIITEAVF
                     KYTNFRRYIGLCNVPIHLKNDVAKLFNVESDRISMDFAGLNHMVYGLNVSLDGEDVTK
                     EAIDKFVKADISMQNIKAIDFNAEFVKSLGAIPCPYHRYYYKTKEMLEDELREFKEGK
                     ARGQVVKELEEQLFELYKDEKLDVKPPQLEKRGGAYYSDAACNLISSIYNDKKDIQVV
                     NTLNKGSIRDFKDEQAVEVSSVITKNGPKPISIGYLPDSVHGLVSQIKSFEVLAAKAA
                     VYGDYESALLALCINPLIPSDDLAKTILDEMLEAHKDYLPRFNR"
     gene            complement(44141..45454)
                     /locus_tag="PC414_000451"
     CDS             complement(44141..45454)
                     /locus_tag="PC414_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2462864.1"
                     /translation="MEKFMSFMDKYIVPVAAKIGAQRHLVAVRDAFIVMIPITMVGAL
                     GTLINNLPLEAYKNLMASIFGENWTTFGGDLWWGAIGTMAVFLVIGVAYFLAKSYESD
                     GLQSGLIALSIFFIMAPQIGKIVPEGGTTVVEGWGMIQQTYLGTAALFSSILIGLLST
                     EIFVRLSKVKKLTIKMPDGVPPAVSRSFAKLIPGMLTIMIFTVIGIFIKMLSNGSFLT
                     DILNTYLGAPLSNVADSLGSTMLIAFIIHILWTVGLHGANIALPFTETILMKLGGENA
                     ALAQAGATEGYHVLAGAFFDAFVYLGGSGMVLGLIVALLIAGRRRKEMIVLGGPPAIF
                     NIGEPLIFGLPIVLNPIFMIPFVLAPVICSAVSYLAIDFGLVAPVILPKIPWVTPPIL
                     GGAMATGDWTGGALALFNLILSILIYIPFVIASEKMEANKLKINN"
     gene            complement(45476..45787)
                     /locus_tag="PC414_000452"
     CDS             complement(45476..45787)
                     /locus_tag="PC414_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2462865.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMEDAAKENGIEAKIWAISEVNLKN
                     EIENCDVLLLGPQVRYVLGKATEMAKPYNIPVEVINMMDYGRCNGKAVLDRAVELNSS
                     K"
     gene            complement(45987..46691)
                     /locus_tag="PC414_000453"
     CDS             complement(45987..46691)
                     /locus_tag="PC414_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2462866.1"
                     /translation="MKEPIYKVIENHVRELINSDSLKEGDLIPSEKQLSEEFNVTRMT
                     VRSALNNLVKEGYITRQRGVGSIVIANNIYDNISSVSGFTKEMESKGYKVSNILVSLE
                     IVQADENLSSKLNISLEENVWEIKRVRLANDVRVSYMITYMPVKLFPNLNKTHCENSL
                     YNFVEEVCGYKIAMSEREVQAVISNEECMDNLKLEEPEPLLYISQICKLQNSEIFEYS
                     HTYHYGYTLTLNAVVE"
     gene            complement(46782..48143)
                     /locus_tag="PC414_000454"
     CDS             complement(46782..48143)
                     /locus_tag="PC414_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2462867.1"
                     /translation="MKDRLNLTEGRITEKLLKLSLPIMGTSFIQMGYNMIDMIWVGKA
                     GSKAVAAVGTAGFFPWLAMAFIMISKVGGEIKVAQSIGANNISTTKSYMKSAIEINII
                     LAIIYTISLIVLNKQLIGFFRLGDLEVITMSRQYLIIVALGMVFYFINPVFTAIFNGL
                     GNSKTPFRINTVGLITNIILDPILIFGWGFAPKLGVAGAAIATVFAQIVVTTCFIYII
                     VKSKEEYFKLRLFKEIETKYYKVLYKLGLPIAIQSGMFTVFSMLLGVIVASWGPVAVA
                     VQKVGSQIEAISWMTAEGLSVALGSFVGQNYGAKKYSRINKGCKIAITVSSVLGIITT
                     LVLVFAGESIFSIFIDESEAIEKGAVYLKILGYSQFFMCLEIITTGAFKGLGRTYIPS
                     IIITILTGARVPLAYFISRPEMLGLNGVWWSITLSSILKGILMISLFISLLKLGKLYK
                     ISE"
     gene            48642..49796
                     /locus_tag="PC414_000455"
     CDS             48642..49796
                     /locus_tag="PC414_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2462868.1"
                     /translation="MKINKKFNRVDMQVSILTAIIVIISSSCVYFFNYTLTYKSMIYS
                     LSERSKHIYEYVERNIDKNTFTEINSKEDQSKKSYKSSKILLESVKNTTGVMYLYTAK
                     KTKDGSLVYVVDGLNSSRSDFRNAGDLIEKEIWGDLNKALDNHILLPKKIKSTSWGYI
                     FISYFPIHNDVGKVVGVIGIEFEAKHQYNTFKFISIVTPIIALLTCLISALIAVILFK
                     RISNPTYTDFANTDYLTGLKNRNAFEIDMNNLNNSNLKNLISIISIDLDGLKKINDKF
                     GHQTGDLYIKQASKIIQYVINKKYIVYRIGGDEYIIILKNLSHKEINNIINNINLKIV
                     EENSTNELKLSMSIGYAIFDEKVDVSLFDTYRRADSQMYDNKRKINKQYQ"
     gene            complement(49878..51596)
                     /locus_tag="PC414_000456"
     CDS             complement(49878..51596)
                     /locus_tag="PC414_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HDF2462869.1"
                     /translation="MNNNAISSKSISLKSKMNLDIKKCMLTVLVMSLVIFLLAPIIYL
                     FVQAFLDKDGVFVGLQNFQEYLASESLLNSLKNTVFVSVISTVISLTLALVYAYALTR
                     TKIKFKGFFKSLAMFPMFAPTMMHAIGLVYLFGNQGLVTNLLGVHVPIYGAVGIILAQ
                     IIYVFPQSFQILYISLSSTDYRLYEASNTMGISKIREFISITIPSIKYSLISSAFVSF
                     TLCFTDFGAPKVIGGEFNVLSIDVYKQIIGQQNMPMGATVSILLLIPAIISFIVTKKI
                     EKNQNSYITSKSIPYKVKTNKLRDSILGGVVGIIAFGIIAVMLTVILSSLISVWPYDL
                     RLTLEHFSMKNSIDGIKPFINSIKMSLLTAILGTSFVFLFAYLNEKTEKAPYLKSFGY
                     FLSTLPMALPGLVLGIAYVLFFNKLEFNFMDTIYIKNLFNGLYGTLLIMVICNIVHFF
                     SVAFITATTAIKKVDKELDMVAKSMGINSASILKNVTIPLCLPAVLENFMYFFLNSMV
                     TISALVFIYTASSKVAAISIVQLDDKGSTAQAASLAVLILVTNILVKVIYEFIKNRLE
                     KRTNFN"
     gene            complement(51601..52590)
                     /locus_tag="PC414_000457"
     CDS             complement(51601..52590)
                     /locus_tag="PC414_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2462870.1"
                     /translation="MSYLKINNVFKSYDQKRVLNNISLDIEEGEFLCLLGPSGCGKTT
                     LLRIIAGLEDVNSGTIILQDKDITNLEPSKRGFGIVFQSYALFPNMTAYNNIAFPLKE
                     RKVSKGKIDNKVKEVLETVGLTNEAHKYPKALSGGQQQRIAIARALALEPKFLLLDEP
                     MSALDAKVRHKLRMDIKRLQKELNITTIMVTHDQEEAITMADKIAILNGGDIMQIGTP
                     EEIYQNPQNLFTAQFIGDTNCFDNGDSILTVRPEYVQIEKSTKENYQGIISNIEFRGN
                     LLRVEIKDKLNENFIISDASIKEWVNLNLVEGDLVKISIDEKYYLKYPKVKGA"
     gene            complement(52686..53735)
                     /locus_tag="PC414_000458"
     CDS             complement(52686..53735)
                     /locus_tag="PC414_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2462871.1"
                     /translation="MKEFIFTCLCIVVITFMTSCISTTPQTTQGKGNNKLTIYAALEE
                     EQIAYYMEGFREEYPNIDVEIVMDSHGVIASKLLAEKDNPQADIIWGLSAMNMIELKK
                     DGALEAYKPKNYDKVDKKFKDTEDDVSWVGMSVPETAIVVNYKELKKLGIDIPKSYED
                     LIKPEYKGLITMPNPASSGTGYLTVSGLIQMMGEKDAYNYMDKLHKNIATYTHSGSKP
                     AKLAGSGEYPIGITLGYRATQQLSSGEPIEVVFPKEGSGWDLEANALVKKDNIKKEAK
                     LFLDWAISDSAMDRYAHEVAVTSIKTDNPVPKGYSKQPFEQLIKEVDLNSAAKNRSTV
                     LKTWESKYGSKSESK"
     gene            complement(54012..54680)
                     /locus_tag="PC414_000459"
     CDS             complement(54012..54680)
                     /locus_tag="PC414_000459"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HDF2462872.1"
                     /translation="MARLNINPTRMEMTRLKKLLKTATRGHKLLKDKLDELMKQFLEI
                     VRENKRLREEAENALDTAYKNFIIARAVMSQEYLGSALMMPKQSVSVDVSTRNIMSVD
                     VPVFDFKTENNQSDIYPYGLAFTSGELDSAMEAFSDAMQPLLRLAESEKSAQLLAQEI
                     EKTRRRVNALENVMIPNYIETIKYIAMKLEENERASTTRLMKVKDMVLKKALEEKKKN
                     DLVV"
     gene            complement(54686..56059)
                     /locus_tag="PC414_000460"
     CDS             complement(54686..56059)
                     /locus_tag="PC414_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HDF2462873.1"
                     /translation="MIKEYKSIQEVAGPLMLINGVEGVKFDELGEIELSNGEIRRCKV
                     LEVNGDTALVQLFESSTGINLAESKVRFLGKSLDFGVSPDILGRVFSGMGRPIDGGPE
                     IIPDKRLDINGAPINPAARDYPAEFIQTGVSAIDGLNTLVRGQKLPIFSGSGLPHANL
                     AVQIARQAKVRGTDSKFAVVFAAVGITFEDAEFFISDFKATGAIDRSVVFVNLANDPA
                     VERVSTPRMALTAAEYLAFEQDMHVLVIITDITNYAEALREVSAAKKEVPGRRGYPGY
                     LYTDLATMYERAGKMKGHEGSITMIPILTMPEDDKTHPIPDLTGYITEGQIILSRELY
                     KKDIQPPIDVLPSLSRLKDKGIGKGKTREDHADTMNQLFAAYSRGKDAKELMAILGES
                     ALSEIDLMYAKFADEFEKEYVSQGFRTDRTIEQTLEIGWKLLSMLPKSELKRIRDEYF
                     EKYMPKE"
     gene            complement(56056..57834)
                     /locus_tag="PC414_000461"
     CDS             complement(56056..57834)
                     /locus_tag="PC414_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HDF2462874.1"
                     /translation="MKTGTIVKVSGPLVVAEGMRDANMFDVVRVSDKHLIGEIIEMHG
                     DKASIQVYEETSGLGPGEEVVSLGMPMSVELGPGLISTIYDGIQRPLEKMYDISGTNI
                     TKGVEVSSLDRTTKWEFKPKKSVGDKVVAGDIIGGVQETAVVECKIMVPYGVKGTIKE
                     IYSGEFTVEDTVCVITDEKGNDIPVTMMQKWPVRKERPYREKKTPDSLLVTGQRVIDT
                     FFPITKGGVAAIPGPFGSGKTVTQHQLAKWADADIVVYIGCGERGNEMTDVLLEFPEL
                     KDPRTGESLMQRTVLIANTSDMPVAAREASIYTGITIAEYFRDMGYSVALMADSTSRW
                     AEALREMSGRLEEMPGEEGYPAYLGSRLAQFYERAGKVNCLGMDEREGTLTAIGAVSP
                     PGGDTSEPVSQATLRIVKVFWGLDASLAYKRHFPAINWLTSYSLYQEKVDVWADKNVN
                     DNFSAQRAQAMKLLQVESELQEIVQLVGVDSLSDGDRLILEVTRSIREDFLHQNSFHE
                     VDTYTSLHKQYRMMGLILKFYKSAQDAIKQNVTINDIIKLSVLEKIGRAKYVEEGDID
                     AAYDSIEDEIDNELADLIKKRGAEEL"
     gene            complement(57951..58274)
                     /locus_tag="PC414_000462"
     CDS             complement(57951..58274)
                     /locus_tag="PC414_000462"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HDF2462875.1"
                     /translation="MLMYKVGVVGDKDSIMGFLALGIDIFPAYDSDEIKKSIHKLVED
                     EYAIIYITEQASLLAKESIAKYKDYQLPAIIVIPGIGGSMGLGMNEVRESSKRAIGAD
                     ILFKE"
     gene            complement(58261..59238)
                     /locus_tag="PC414_000463"
     CDS             complement(58261..59238)
                     /locus_tag="PC414_000463"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit C"
                     /protein_id="HDF2462876.1"
                     /translation="MAEEKTYPYAVARIRVLEKQLLNRQMFIQMAEAKSPEDSLRIIA
                     EAGYADTSNNNIHNFENILTQELSKTYEILKSLAPEEKFVDVFLYKNDYHNLKVLIKE
                     EISGVDGEKYLIDGGTIPLIKLRESLANRSFSDLPKIMSSAILEAFDVYNKTQNGQMV
                     DIVLDKATFTSMKETAKESQNKFVIDYVMKVCDLTNLKSFIRVKNMKKDFDMFMNVFV
                     SGGSLDKEKFLEAFSSDTPASCFKSTSYSDVCKNGMDSGFTVFEKLCDDYLMEYVRGA
                     KFKPLTLEPLIAYLYAKESEIKTIRIILTSKLNNIDADTIKERLRDAYV"
     gene            complement(59253..59816)
                     /locus_tag="PC414_000464"
     CDS             complement(59253..59816)
                     /locus_tag="PC414_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HDF2462877.1"
                     /translation="MGNEQKMIDRIIADAKQEAQEILDKAKSEADLKVNSANEKAEKE
                     MASYTKLAEAEAEKAASKEISGAYMEAKKQILSKKQEILEEVILEAKNKLLNLKDNEY
                     EEIILNMIEKSNCTDDSEIVLSKKDKKTLKDVLSKKGIKVSDETRDITGGFIVKKGDI
                     EYNYSFEAIIAVEHEYIEQIAAEILFN"
     gene            complement(59868..60359)
                     /locus_tag="PC414_000465"
     CDS             complement(59868..60359)
                     /locus_tag="PC414_000465"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HDF2462878.1"
                     /translation="MEFLNALGNGQFLAISGAAIAALFAGMGSAKGTSIAGQAAAGVV
                     TEDPSKFGQLLVLQLLPGTQGLYGFIIAFLILSKAGIIGGDAIPTSAQGLQLLMAGLP
                     IGLVGLVSGIAQGKAAAAGVGIVAKRPEELGKAITFAAIVETYALLGLLVSFLAYNGI
                     AIG"
     gene            complement(60362..62287)
                     /locus_tag="PC414_000466"
     CDS             complement(60362..62287)
                     /locus_tag="PC414_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HDF2462879.1"
                     /translation="MAIVNMNKISIVGLEAQKSQILKLLMNRGFVQIDDSAFLTEEEE
                     LKNYLVKDSEESEVIMLEQKMSHVNQAINIVSSLVKQKKSLLAPKREFNKIDSEKEGQ
                     VYAEALELNNLHKEIGTIRSNINLLNNNKSMLEPWVNFDIPLENMETKYTKTILGTVP
                     ISANIDALRVKLEDEIPESVIGIVNSDKLMNYVYLITLKETFDDVLEVLKEFNFSVVS
                     LPNEEGTPLQAIKKYNEMIIRQENVSKDKTEEIKRHADSIHMLENLYDFYTIERDQKK
                     IIERLVKTKTTFCLNGWLPSKRADELIKEITEKFNCCVQTEEGSKEEGFPILLENNKL
                     VTPFESVTNMYSYPSTKDIDPNTILTFFFVVFFGMMLSDAAYGLIIAVVCGFVVYKLK
                     IQKGEGNLIKLIGICGVSTTVWGLIFGGILGDLIPIKALINPLEDVMTLMGMSLLFGI
                     IHIYVGLGIKAYTLIRSGKVIDAIFDIGFWYLCITGVCLLIIPFVAGDIGVFSEVGKY
                     LAIIGAIGLVLTQGRSFKGIPVKLFKGFSSLYGITSYFADILSYTRIMALCLTTGVIA
                     QVINLLGAIAGPILAVVIGVVGHTINLLINALGAYVHTSRLQYVEFFNKFYEGGGVPF
                     VPFKYKTKYTSINKKEM"
     gene            complement(62289..62600)
                     /locus_tag="PC414_000467"
     CDS             complement(62289..62600)
                     /locus_tag="PC414_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2462880.1"
                     /translation="MAFEIIKSIVEAEQTADSIKVKAVTDAESIRADAVNKCESIFAD
                     VKKQAKLMEETLIEKAVTDSRAEVDKILANAKSECLKIEKTAEERKSKAIEAVIGKVV
                     R"
     gene            complement(63230..63505)
                     /locus_tag="PC414_000468"
     CDS             complement(63230..63505)
                     /locus_tag="PC414_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif11-like leader peptide family natural product
                     precursor"
                     /protein_id="HDF2462881.1"
                     /translation="MNEKLTKILEEAKNDDEFRKELIKTREQRDVMDSFCKVVTSRGY
                     DMTVGELFSMGEEYTSNLLKSVNGGAAYPIEDWSDWYEDFFIMLSNI"
     gene            complement(63620..65029)
                     /locus_tag="PC414_000469"
     CDS             complement(63620..65029)
                     /locus_tag="PC414_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HDF2462882.1"
                     /translation="MIDGKEVKQESGQKNLKFKIAIVVGVLFVAYIGMAIFFRNHFYF
                     GTTINGVKASGKTVEQVNDLLTSSTNSYTLNLEERNNKKEQIKAKDIDLKFTPDDKVQ
                     KIKDSQSAIGWIGGIFKSKEYNNMITLSYNKDLLEKRFNALSCFDSKNVVEPKSAYPK
                     YDSTKNAYVIVPEVLGNKVKKDDFYKLVQNSINSGNTSISLDESKVYEAPNNTKDSEK
                     LKQAIKTLDKYVGVVVTYDFSDRKETLDGSEINKWLKVDSEKNYDITFDNDAMVEYTR
                     GLSKKYSTFGDSRPFKTATGKDITISGGIYGWLIDKKKEAEALVDVVKAGKNVTREPI
                     YIQKAVSRNKDDIGNTYVEISLSGQHMWFFKNGEMLVSTDVVTGDLARGFATPSGIYP
                     LNYKARNVSLTGQGYSSPVSYWLPFNGNIGIHDATWRNSFGGSIYKSSGSHGCVNTPF
                     SKAKKIYENIEPGTPIILY"
     gene            complement(65400..66062)
                     /locus_tag="PC414_000470"
     CDS             complement(65400..66062)
                     /locus_tag="PC414_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HDF2462883.1"
                     /translation="MIDISIDDKLKERWPNAKLGCIQAKVAVNKSSEKLINEINEFCD
                     TINQSLKVEDITKLDKIKDARNAYKELGKNPSRYRTSSEALVRRIIQGKGLYTINNIV
                     EINNLISIKSLYPVGTYNVSKLHSPVCFTVGDEGEQYKGIGKELINIENLPILSDSVG
                     KFGSPTSDSERAMITEDANEILICIFSFSQESDIEEYFGYAKELLKEYADGKDIETKI
                     IK"
     gene            complement(66316..68535)
                     /locus_tag="PC414_000471"
     CDS             complement(66316..68535)
                     /locus_tag="PC414_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2462884.1"
                     /translation="MTGRNKSFTLITILFLTSLFLTMGYIYIEDTKHLLMSEAKIHIK
                     EVAVQGSQLAQRQIEKDLDVLNIFSNYYASNPDIPNEEKMKNLLDEMENQKFYTMAIV
                     DINGNAENTKGDKFSVKDREFFKNSIKGKKYVSSPYVDEVNKSIKKIAISVPLLNNDK
                     VVGVLYCTYNINTLMKLINISFYENNSISYVVKNNGTIILHPQGDSLSKNIYKLLKQD
                     NDIQEVNRLKKELQENKTGATVLNMLEERRYLGYATMDNGNSENNYIKDWNLIFSIPE
                     TVVFSNSKQIINRAVYAVLSIVLIFIAIIFYIIYIKKSNEKEILSLAYEDKVTYIGNQ
                     NKFYRECSKYLLDKPSLNYIIVYFDINNFKMINDTFGYEFGDNLLITIAKALKEELTE
                     GEVYARLSSDYFAIFCDYKNGRNKIIRKLDNIRSNIESNLSIVFEISLCVGIYFVEEG
                     EVDIQKAVNKANMARSVAKGKNINYAIYNEDVRNKLSEESMILDDIKIALVKNQFEVY
                     YQPKFSLVTGEMIGSEALIRWNHPEHGFISPAVFIPIAEKSKLILKIGRFVFERVCND
                     LYEWKKQGKKIVPVSVNLSRVELYQPDIVKFINKTIKMYNLSSDFIEIEITETVAINE
                     LNILKNVLNELRTYGFSISMDDFGTGYSSISCLRDMPIDILKLDKSFLGGIEHDERSR
                     NIAKSIVSLAKSLDLVVIIEGVESKEQAELMKQFGCDLVQGFYFARPMPAKNFLDLL"
     gene            complement(68811..71465)
                     /gene="adhE"
                     /locus_tag="PC414_000472"
                     /gene_synonym="adhC"
     CDS             complement(68811..71465)
                     /gene="adhE"
                     /locus_tag="PC414_000472"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDF2462885.1"
                     /translation="MAHKEQVIIENKKENEVMSVGLDTVNNVESLVKKLAKIREAQKI
                     FATYSQEQVDKIFLAASLAANKQRVPLAIMAQKETGMGIAEDKVIKNHYASEYIYNAY
                     KETKTCGVIEKDEAFGMTKIAEPIGVIAAVVPTTNPTSTAIFKALLALKTRNGIIFSP
                     HPRAKNSTIEAAKVVLEAAVLAGAPEGIIGWIDEPSLELTTTVMKEVDLTLATGGPGM
                     VKSAYSSGKPAIGVGAGNTPAIIDDSADIKTAVNSILVSKTFDNGMICASEQSVIVLE
                     NIYNEVKKEFKERGAYLLDKDETEKIRNIILVNGGLNSKIVGQTACTIAKLAGFEVPV
                     DTKVLIGEVESVEIEEAFAHEKLSPVLAMYKASDFDDAVRKAEKLVEDGGFGHTSSLY
                     IDDVNQREKLDKFTSAMKTCRILINTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVS
                     ENVGVKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVALKELKDVMNKKRAFIVTD
                     TFLYNNGYTKAVTDLLDEMNIKYTTFFEVEPDPTLECAKIGAKAMREFNPDVIISIGG
                     GSAMDAGKIMWTLYEHPDVDFQDLAMRFMDIRKRVYTFPKMGEKANFVAIPTSAGTGS
                     EVTPFAVITDQDTGVKYPLADYELMPNMAIVDSDMMMNMPKSLTSASGIDALTHALEA
                     YVSMLATDYTNGLALQAIKSIFEYLPRAYDNGAKDPEAREKMANASTMAGMAFANAFL
                     GVCHSMAHKLGAFHHVPHGVANALLITEVMKFNSSDAPKKMGAFSQYKYPEALKRYAE
                     IASFLGLKGNSDEEKFQSLLVAIEDLKLKVGIPKSIKEFGVEESKFMDSIDEMVIQAF
                     DDQCTGANPRYPLMNEIKDMYLNSYYGR"
BASE COUNT        23688 a        12595 c         8284 g        27286 t
ORIGIN      
        1 ttaagtcctt catttcctcg tttttatacc cacatttatt gcatatttgt gaacttgcaa
       61 agaatttacc tacctttaca atttgtctac catgccaatt agccttgtat tttaattgac
      121 gaacaaattc tgaccatgac acatcagaaa ttaagcgaga tagtttacga tttctaatca
      181 tattctttac ttgtaaatct tctatgcaaa taatatcatt ctcttttatt aatttagtag
      241 atagtttttg tagaaaatca tttctttgat tagctatatg ttcttgaagt cttgcaactt
      301 ttaatcttgc tttatttcta tttaaactac cgattgtctt tctcgataat tctctttgta
      361 atttagcaag tttgtttaat gacttcttaa gatacttagg attttctata aagtctccac
      421 aacttgagat acaaaattct ttaattccta aatctaaacc tatctgatta ttagtatttt
      481 caaatacttc aatatctaca ttagtacagc ataatgatac ataatatcta ccacttggtt
      541 ctttagatat agtagcatta agtattctac cttgaggtac ttgtttatcc cttaccttaa
      601 ccataccaag tttaggtagt tttatatatt gaccacaata cataatgttt ccatttgtaa
      661 agttagtttt atatgaaaat ctattaattt tctttgattt aaatttagga aatcctgctt
      721 tttctttaaa gaatttttta tatgcattat ctaaatcttt taaggcgttt tgtagtgaaa
      781 atttgtcagg ttccttaagc cattttaatt ctttttttaa attggttaaa tcagaactac
      841 actgtttata agtaaaagtt tctttattgt ttttataaac ttctattctt ttagcaagat
      901 acttattata tacaaatcta caacatccaa aagttttgtt aattaactct tgttgttttt
      961 tatttggata cattctaaac ttataagctt tttccactac tgccacctca ctttacttgt
     1021 tttttctgat tattaatata ttttcttatc tgttcttctg tattttctga tacagtagca
     1081 acgaaataac taggattcca aagatgtcct ccccataatt tctttttcaa ctgttctcca
     1141 tgttttttca ttaacagcct tgctgataca ccttttaatg cttttattat atctggtata
     1201 tagtgttgtg gtgaacaatt tattagtaaa tgtatgtgat ctttatcact attacattct
     1261 aatatttgaa atttattatc ttcagctatt ttgtttaata tattaattaa atcttcttca
     1321 atttcatttg ttaatactgg atgtctatat tttacacacc atactatatg gtattgtact
     1381 aaatacacat accctctacc atgagtcact tccatttttc atcacccaac ataatattaa
     1441 catagtaatt acttaaaaca agtattctta aggtttttat atttattttt aaataacata
     1501 cgtaagaaac gcaaatctta acttttatca tttttgaggt tgtcgttcac ataagttcgc
     1561 tactacctat gcagttctct tatgaacttc ttacactttc atgcaagcac agactatatc
     1621 ttatccttcg gcattatccg ttaaggtcta cccacttcca ctaccgatag cttgtagtgt
     1681 acttccctca agaggaatag tcgttgaagt ttttcctatt cggagcttac ctgctgattg
     1741 cccattttaa agtacttagg atttaacctt ataccatcta accaattttt tctactttcg
     1801 taactttcac acttaggttt atttcatcct tatgttttag tttggttagc tttaggggtt
     1861 tccagcaatt caagtagtat tggataggtt ctatcctatc tctacatata agtttcccta
     1921 tatgctgact attttagcta ctaactcatg actaaagtca caagtgtgca tagctatttt
     1981 ttaatcaaga gaagtctctt ctttcataga caatattaac tctattcttt ctagtatttt
     2041 atctcttgga aagaaagtct cttgacctgt aaaagctgat ttttttataa accattcctc
     2101 tggtattatg ttttttcttt tccatctata taattgtcca taagatatat tagttttgct
     2161 caataaatct ttcttagata ttaattcttc catagctctt ctagcccacc tttatactgt
     2221 tttttacagt agaattatct gaaactgata aattatcact gtattcaata tttccaacat
     2281 cataattatc tactagagat atgctttttt tctagcatac gaaatttttt gcaataatct
     2341 ccatgcccct tttttatatt tatactactt gctccaactt cattaaaatt agaagttcca
     2401 tttaaactaa tattaacttc ttcacaattc aaaaatccat tacactccaa gattccactt
     2461 aaattgatat tttcacaatc tatattctca tttgctttta aatatccttc tccatgaata
     2521 atatccgatt ttatacttcc ttctacatta ataattccac tagtatcaat ttcaacagaa
     2581 ttaatatttc ctgtacatct aagaagtccc gatatctcaa cttttgatgc cagtatgttt
     2641 ccttctactt tacttaaacc agatgtaaaa aattcattac attctgttgt cccaaaaact
     2701 tttgctgaac cagaagaatc aaatttacta cattttacac ttccttctat tttgcctgtt
     2761 cctgatatat taacattatc aaaaattcca cctttacatt ttccaacacc atcaaatctt
     2821 agtgaactta attcttcatt cataatgata tcctttcttt accgtatttt atctttaaac
     2881 ctaatataac aaaacattgt tacgttgtca agtatgataa taatacttaa taaagcaact
     2941 atcatttatt ttctataaat aggcataaaa ataataggga aatgctttat ttaaactata
     3001 aatctaattt cttagattgt ggtttaaata aaatatatcc ctattactca atgtttatat
     3061 attcattgta taaacttcta aaatataagt ctttaaattc tactgtcaat actttttata
     3121 cactgtttgt gctatcatct cacacaccta aaggagtagg ctttgcgcac tattctgtaa
     3181 cctacaagac actatcttct tctatctatt gataaatttt tctattacat cagctagtct
     3241 ctccatatca gttggataga cttcacctat gctacctatt ctaaatgtat ctatatcagt
     3301 caatttacct ggatagataa caaatccatt atcttttaaa taagtataaa attccataaa
     3361 ttcaaacttt gcatttttag gatataagaa agttgttatt acaggtgatt gagcattatc
     3421 attcaccaaa gtatcaaagc ctaaagattt taatcttgaa gctattgtaa actgattttc
     3481 tttatatcta gcatatcttt tttcaacaga accttcttct tccaattcta ataaagcttg
     3541 ataaaaagct cttacaacat gtgtaggtga agtaaatctc catttaccat tatttttctc
     3601 catagtctcc cattgcgcat acacatcaag agataatgat ttagctatac ctttacattt
     3661 agataatttc tctttattag ctattataaa accaaatcca ggtactcctt gtatacattt
     3721 gttagaacta ctaactaaaa aatctatatt aaaatcttca acatcaatct caattccccc
     3781 aaaactactc atagcatcaa ctatataaat tttgttatat tcttttgcaa gtttccctac
     3841 ctcttgtata ggatttaatc tacctgtagt agtttcacag tgaaccattg atatatgtgt
     3901 tatatccttg ttttctttta ataaattttc tactacattt aaatccactg cttctatatc
     3961 tttaaatgtg cagtcaacaa attgtatgtt aagataatta catatatctt tcattctctt
     4021 tccatatgct ccatttgcaa ttacaagaag tttaccatct tttgaaatag tacttcctat
     4081 tatagcttct actgaaaaag tcccacttcc ttgcattaat acacttgtgt atttatctgt
     4141 attttgggtt gctagagata ctaatcttct tctaacatct tgtactaggt tattatattc
     4201 aacatcccaa gtacaccaat cttttaacat agatgctctt actgatttag tagtagatag
     4261 tggtcctggt gtaagtaata tgtaatcatt ttctggtaca aaatctgatt taatagtttc
     4321 attctctatt tttattagta tatcttctaa ctcagccata gtttctatta caaaatgtgc
     4381 acctgcctct ttaaatttct ttgaaactat actcatctta gcttttaatt cttccttatc
     4441 catgttctct acttcttctt gtgtaagccc taactcactt gaacctttta ttacagcaac
     4501 actccacatt cctgcattta cgccttcttt tacatcactt attgtatctc ctacttttac
     4561 catagaactc ataggagaca cccctaaagc ttcagcattt ttatagcaca tccaaggata
     4621 cggtcttcct tgagatactt ctgatggtgt tacaagaaaa tcgggtgagt atcctttctt
     4681 tgctgcattt ggttcaacta tgttcatcat ctctcttgta taacccgttg tagaacctat
     4741 ttttaatcca ttttttctca acttttcaat agtttcaaca acatgaggaa ttggagtcgt
     4801 atactcttct aatgtctcaa ataacattgg ctcaaattca gcataaagtt catttacatc
     4861 atcttcagtt ggcactttac caaacttatc tgaccaaata ttctttattc tatccatttc
     4921 acacatttct cttatatggt ctatttttaa ttttcccata ggttttcttg cttcttccat
     4981 tgttacatct atacctcttc ttttaaatat ctctataaat acattaagag gtgcaaaaca
     5041 tccataatct actgttgttc cagcccaatc gaatactact gcttttattt tttctccata
     5101 tatctttttc atatttatag cctccataga ttctttttag aatttttgtg tttttaattg
     5161 tttttacttt taaagtataa ctttcatatg ttaattatac atcatattta ggttaaatat
     5221 atattaaatt tctgttttta gttgtattta gttgttttta ttttgtatca tattcccata
     5281 aaatcctaag ttacaatcta aaatttactt tctaatctta atctatgaac tgactatatt
     5341 ttatgaattt agttgtattg agcatagttg tattatataa aactaagggt ttattttgct
     5401 tctatccatg ttatatttat ctcatttaac ttatccttcc attcattcgg aggttctaca
     5461 tcacaaataa caaaatctaa ttcctctaaa tctgctatct tatacatact agcaattcct
     5521 attttagaac tatcacataa tacaaaactt tcctttgaat tttttatata ttcttttgac
     5581 aacaatgcct tattttggtc tatactacta attccaaaac tatcaagtat accttcacta
     5641 gatataaaag ctttatctac gtataagttt ttcatagtat ctattgacat tggacatgct
     5701 gtcctttggt gttttaaatt tacttttcca ccaataaata taacttctcc atcaaacaaa
     5761 ccttgattct cataagaaat caattttgat agaactggat aagagtttgt aataattttt
     5821 aaattgttta cagatattat ataatctaca atctgcaaat tggtagtacc ttcatctatg
     5881 ataattgact cattgtcatt tacaaactta gctgcatatt tagcaatact ttgcttttta
     5941 tctaggttag tattgtttct ctttatatgt tctggttcaa tcactccata atttattttt
     6001 atagcaccac catatacctt ttttaattta ttttcatttt ctaattcttc caaatatctt
     6061 cttattgttt ctgatgatac ttctaatctt tttactaatt ctttagtgtt taccttccct
     6121 tttgaatcta ataatttaag tatttctttt tttctatcta tcgaatttaa agacattcat
     6181 tttcctccta aatataatat atttttctcc tataatatta caatgatacc taagatttat
     6241 gaaatcatat ttatgtatta aattttttac ttaattaaag ttattttcaa aagactttgt
     6301 attatcatta aaaaagtcca tcaaatatta gtatcagatg gactttgtaa aattttattt
     6361 tataaattaa tatttaaagt ctaactacta atttattttc ttgtctccaa gaattttact
     6421 aaattattta cagtagacat atcttttctc tctaaatcag ttggttgaag ctctatccct
     6481 aatctatttt caatttcaag taaaacttca attatagcta atgagtctaa aagttccgtc
     6541 tcaaataaat taaggtctaa atcttctctt atctcatcag tccctaatac atcttcaaat
     6601 atttctataa ctgtttcttg cataaatttt cctccttaaa attaagctaa ataacctgag
     6661 aatatcagca atccaaaaca cacaatatgg aatgtaatga aaatctgcac tctctcgaac
     6721 catttatctt ttttatgttt tttataaaat ttagattttc gctgatatat atcagttaac
     6781 acaagtgcca atccttggta tacaccataa gcaacataat accaagttag accatgccaa
     6841 aatcccattg ttatcatggt tatcatctga gccacatgag cagcagtagt ccttttctta
     6901 aatcttttct ttctcattga tgacataaca aatctagaaa aaatataatc tccaaaccat
     6961 cttgaaagac ttatatgcca tcttgtccag aactctttca tatctttact gataaatggc
     7021 ttgtcaaagt ttataggtac ttgtattcca aatatgtatc ctgtacctat agcaaataaa
     7081 ctatatcctg caaaatcaaa gaacaggtat aaactatatg cgtacatata acttaaaata
     7141 tttataaaac tcatatcttt aggtattctt gatacccaat atgtatttat caaaaatgct
     7201 ataacaaact tataacctac tcccatgaca atatttttta ttcctggcag taagtattct
     7261 tctatatact cttttctaga gatttgtttt tccaagtcct gttcaaatct tcttgaccta
     7321 tcaataggtc cagaacttaa agttggaaaa aataacataa aataaagcat ctttgaaatt
     7381 ttaacttctt ttatagctcc atcataaatt tcaataacca tttgtatagt tctgaaattt
     7441 aagtatgata ttccaataaa tccaattatt ccaaaagaag taactggaga tattttattt
     7501 attattattg ggagcattga tgcaaacaag aatccccaat atatgtattt atttttagtt
     7561 tttcttctta catattcata tcctttaact attattactt caagaataat aaatattacc
     7621 aagtacttta gttggacatc aattcctact attaagaata tcataaacaa acttgccaac
     7681 ataccataat actttatatt cttgcccatt agacctaaaa ttactgctgg tattgatgtt
     7741 aataataata tataaagata aaaataatct ccatattgtg aaaaaatcat taaatttcct
     7801 ccataagttt ctttctgtct attttaccat tgatatttgt tggaaactca gagattattg
     7861 aaatatttct tggaatcata tatgaaggta tatactttcc aagttccttt tttattatca
     7921 tcccattttt aatattactt aaatcattct tttcatttaa ctcaacaata ccttttaagt
     7981 aagcaatttt ttcgtcctta tatactggta aaactactgc attttttaca ttatgtactt
     8041 ttctcaaatt attttctata tcttctattt caattctaaa tccatttaat ttaatctgaa
     8101 aatcttttct tccacaataa tatatatttc catctaaaag atatcccata tctccagttt
     8161 tataagctct ccactttaca ccatctattt catcatagaa aaatacttca tcagtctttt
     8221 ctttattatt gaagtaacct ttagaaactg atggtccaat tattataatt tcaccctttt
     8281 cattttcttt aagctcattt ccatcttcat caagtatttt tattttacaa tttgacatag
     8341 ggtatccaac tggaagactt ttttcatcat ctatagcctt ttgagtcata tcatttacac
     8401 ttacaccaac agtagcctcg gttggtccat atccattgat aattcttgtg tttggaaatc
     8461 tactcataag ttctttagta agattttttg aaagtgcttc tcctatgaat atcattgact
     8521 caaggttagg aagcatttta ctattaaact ctttttcagt tatacatact cctgcaaaag
     8581 aaggagttga cacccataca gctatatctg agatactaaa ttgtctaaat agttctttat
     8641 agtctgctaa tacatctttt gatattgaaa ataatgtagc tccatggata agaccagggt
     8701 atattgttgt aactgaaagg tcaaaagaat atgctggttg gttcattatt actttttcac
     8761 ttccatctat gtttagatat gggcttatcc aatctgaaaa gctatctaaa ttgtttgaac
     8821 ttatttgaac cccttttggc tttcctgtac ttcctgaagt aaatagtata tatgcatttt
     8881 catcatcttt tacccagttt tctttatcta atgattttcc ctgatattca tttattatat
     8941 aatttaattt atccatatca attacattga tatctccaaa atctttttca tcactaaagt
     9001 taaataatac ctttggtttt atctctttag tgacttcaaa aactctatca attggaaaac
     9061 taatatcaag aggcacatac gctctacctg attttaaagc tcctatcata catgccatta
     9121 tcatgttttc tttatttcca taaattacta taggagtatc ttcttctttg tatacatcct
     9181 ttagaaatac tgatatagca tctgaatatt catttaaatc tttataagaa agtttatctc
     9241 cattacacat tagtgctgtt ctatctgtat ttgaatattt ttttattcct tcaataattt
     9301 tcatataaga atgctccttt aaaatccgtt ataaataaat ggcaattctt taaatggagt
     9361 aaatgttaga actcctatcg ccaatattat aaccatagtt aaacaaatac tagttaacaa
     9421 atctttattg ttctttaaat attttaaata atctctcaca gacatctacc catcctttcg
     9481 taccaagatg cattacatcc cttaaataat acttatctga ccctttatct ttaaggttta
     9541 gaacttcaaa gcctttgctt tctgctattt tttgtgcttt atcgtagtac tgatttcttt
     9601 ctttttcaga aataccagtc aaattataga atttatccat acttgggatt aatacaacaa
     9661 ctggcttaat acctaagtca gtacatacat ttagcatcag tttataattt tcaaactcct
     9721 tagatgttaa taaatttaca tctttgtctc ttcctttgta ttgctctata ccatctttaa
     9781 aatgctcttt ataatagtac ttatcaatat ttagtggatt tttaccaact ctttttttag
     9841 catcttcaat ggctttcttt ctctcatatg accaatttat tggtcttttt ctttttgttg
     9901 ctctcttttt atctagctta attaatcgtt tataaagaat acccttttct ttaagagcta
     9961 tacaatactt tcttccttga aaataaggct ctaaaagcac cttttcagct ttttcaggaa
    10021 atgtcttagg ctcatatagc ttagcatata atgcttctgc tttatactcg tcacttcccc
    10081 ataataactt acttgatttt tttgcatatt caatcttatt ttgtttagat atttttggat
    10141 tatctaaaaa tcgataaaat tgtattggtg aaaatctaga ctgataatgg tgacttgtga
    10201 ctccgtcttt ttccataaac cattgcatag aaattaacaa gacaactttc ttattatcaa
    10261 tattaggatt catacttcct aatattgcag catcttgtaa tgtctgagta taggctctcc
    10321 caattgcaaa caccttattt ttactcctat ttgtattaaa atagtacttt ggatgttgtt
    10381 ttgttgaatt actaagttct gatgacccca aaatcataat atccttttct cttaaaaaat
    10441 gattatttgc tgtaacccct ttatctttta tatcacttaa agtatcatcc ataattggaa
    10501 gtagattttt ctccctcaaa agttcatcag ttttagaatc aagaaatttg tctaacccaa
    10561 ataaaaatac tacgcctatt ataaaaggtg taataaagta tatcaatttt ctcatatttt
    10621 ctctcctcta aaaatattca aatttatgtt attattaatc atattagttt tgatatgtat
    10681 attcaactac aaaattgtaa taaaatcgtc aaaacttttt ttgatactgt tacaaaattg
    10741 taaaaaaaaa gaaaaaaacg ccaaaaataa atttcttttt agcacttttt catacttata
    10801 atttgacatt ttaacacttg gttaagtata caccttacct taacttaaaa ttaaatttat
    10861 ttaagaatat atattataaa ttcttatatt ttctaatata aactatatac tatcagctgg
    10921 ataaacaata ctactttgtc ttcttatctc atccataatt tccattacta ttattgaatt
    10981 ttcatgagaa tttatatttg attctatttt attgttattt ataagactta taaactctac
    11041 aagttcataa tacattcctt ctatttctct aggcttttcc tctgcttttt cttttggaat
    11101 agatatatct tcttctcttc catctctgta tttaataata gctttttcaa atagattaag
    11161 cttttcaatt attatacttc cctcttctcc ttgtatttca gatggtatat aagaatcagc
    11221 tactttagaa tactgaatta tcgcatccat atcatcatat tgaaatacag ctgaaccttc
    11281 tccatccact ccagtttgaa gtatatgaga aactgccttt acagtttttg gagcaccaaa
    11341 taaattcacc ataggatgta tacaatacac tcctatatcc attaaagctc catttgacaa
    11401 ctctttttta aaggcatttt ctataattcc atttttatat ttatcatatc ttgaagaata
    11461 ctgacaatag cttccaaaaa atcttcttat tttccctatt ttatataagt tttctttaac
    11521 tgctttaaag tttggaacac atgtaattct catagcttcc ataagcaata cattattatc
    11581 tttggctgtt tttatcattg atttgacttc ttttaaatta gatgctaacg acttttcaca
    11641 aagcacatgt tttttgtgtt ttaaacaagt aatggtttgc tttgaatgta atgcatttgg
    11701 agaagctatg tatacagcat ctatatcctt cgatttagcc atttgttcta aatcatcaaa
    11761 aaaaatactt gctccatata gagttccaaa ttctctagct ttttctaaat ttctagaata
    11821 tacagcaacc aattcaaagt ctttaaccct agatgcagct cttagaaata tatttgctat
    11881 attgcttgtc ccaacaatac caaatcttat tttacccatc ataaatcact ccccattttc
    11941 tacactttat tttaatttta ataaaaaact ttacaactta tagatatatt ataaaataat
    12001 tttatcaaat aaaaaagaaa gctaatttta aatgatttca ccattttaac atagctttct
    12061 tttaactaca atctaaattc aacttatatt tttaaagtta tctttctact atatctttat
    12121 ttcccctcaa aaattttata gtaagaattt catccacaaa caatgttact aaaagtatag
    12181 ttacaacaat tggccaataa ggtattataa gactatataa actatcaact atatacactt
    12241 gaaaatatct aactccaata aatgtcaata tagtccaata tacagaaaac tttagacata
    12301 ctattcctct actattgtac ttataatctg aataatccca atagacctta ttaaaatata
    12361 ctttcataag tattcctgta atatattcta ctactgtcgg cactactaga cataataaaa
    12421 gcaatgcaaa tatattattg gggtcaataa atcttgagac ctctattata atcgacattg
    12481 caattgcata cattggttta aatggaccat ttaaaaatcc atcttcttga aaatgccctt
    12541 gtttataata actatataag ttttcaatta tccaacctat aaaaccatac aagaaaaaat
    12601 taaataaaaa atataataga gaacctacat tatccatgaa tattcctcct agctgtttat
    12661 acttattttt tctcatattc atggatttat tctaaatcaa agaaattata tttaatttat
    12721 ttaattaata ctttatatta tagattgtga cattctttag agttcaaact ttttaggtat
    12781 tctctaactt ccagtaaatt ttcaagtatc ttatgtgatt gtgatttaat ttgctcatct
    12841 ggctctttta aatctggtac atttatacat cttataccac cattgtatgc agcttcaact
    12901 cccataggcg aatcctctat gacaatacaa ttttggggat taacatttat ctttttagct
    12961 gcttttaaga atatctcagg gtttggttta gagtttacaa catcatctcc acaaactata
    13021 gcatcaaagt aatcttttaa atttgcctta gctaatctct ttactgctct ttctctcttt
    13081 gttgatgttg caacagccat tttgtatcca ttttccttta aaaaagagat tagttcattg
    13141 actcctaatt tgataggagc tcccttcctc tccataaatt caatcatatt tttagttttt
    13201 tcgtcatata aatctattat tggtacagaa ctatcgtata tatctgtaag accttctatt
    13261 attcctttac gatttcttcc cataactgat gtataaattt ccttagtcat agtatatcca
    13321 tacttttcaa acgtctccat ccaaaattca agagaaattc tctctgagtc aaacaaaact
    13381 ccatccatgt caaaaataat tccttctact ttttgcatac aattcctcca atattttttg
    13441 atataattat attacacttt ttatgattaa ttttacaacc ctcaatttat ccaattctaa
    13501 tattgtaaaa ttgtaatttt caaaagttac actttggttt actttaatat ttgaaccaag
    13561 catataggat atccacccac ctatactgtc aaatccatca tgctctatat caagtccaaa
    13621 atacttatta atatagttga tagattcagt tccatcaact aagtaagaac ctgctcctat
    13681 tttcttaata tgagtctctt ctttgtcaaa ttcatcttga atttctccaa ctatctcttc
    13741 tagtatatct tctattgtta ccacaccaga tgtaccacca tattcatcca caactatagc
    13801 aagttgaagt ttttcctttc ttattctctc aagagcttta tctatagtta aattttcaga
    13861 tatatatata atatctctca atatttcttc tagttcaatt ttattttcat ttatcttttg
    13921 attgtataaa tctcttatat gtacaaaacc taatatatca tctttatttt ttctgcatac
    13981 tggatatctt gtaacacctt cctcttttaa aatagctaat atcttttctt cagaatcact
    14041 ttcatatata cataccatat cagttctagg aaccattatc tctctaattt tcttttcttc
    14101 aaattcaaaa atattatcaa ctaatctttg ctctgattca tctataagtc cacttttata
    14161 actttcttct actaatagtc ttatttcgtc acctgtatgg ggttcatcaa cctcatcagc
    14221 ttgtgaatat ccaaatggtt taagaaataa atcagtactt aagttgaaaa aatatattat
    14281 tggatatgtt aacttataaa atcctactaa taaaaaagat gtactcaaca ttattttttc
    14341 tgtattataa agtgctaaag ctttaggaac caactctcct attacaacct ccatcattgt
    14401 tataattaaa aaagaaatgg aaattgatat agtatatata atactttcac taagattaaa
    14461 tacacttact attggtaaaa ttaactcttt aactgtagat tctcccatcc aacctaatgc
    14521 aagtgaacaa agtgttatac caagttgaca tgcagaaaga taggaattta agttgtcttt
    14581 tattatttta catctttttg cattattatt accttcagtt atcatggtat caatcctaga
    14641 actacgaagt tttaccatag caaattcaca agctacaaaa aaagcattta tcaaaacaag
    14701 cattactata agtgttactt ttaataactc ccgcataaag tattggttct tataatgtaa
    14761 gaaatctata agaactcaaa gttcccccct tatattaata aatttaattt ataatatata
    14821 ttctatcata tttattaata tatcctactt tattacaaat aatttttata aactaaataa
    14881 ccccttttaa tatttatgac tggctatttg ataaatatta aaggggttat ttagttatca
    14941 ttttttattt ctaaaaatct tttctcaaat gatttagtta tctatttatc caatttttta
    15001 cataatttaa tgattaaaaa tattaatttt ttcactataa agtataaata ttatctgatt
    15061 attaaaataa atattaatac tataaataaa aacgaagcat gatttttatt taaataaatc
    15121 tcaaacttcg tctttactta cttagatatt tatttacttt ctaaatccta ttatttttaa
    15181 aaccttatct ttttaatact tcaccacatt tattctcagt aatctctcca ttttctagcg
    15241 caatttttcc atttacaatt acatattcaa caccattggc tagtaattgt ggctttgaaa
    15301 aatcaatatc ttctgaccta ggataactat agttatccat gtcaataact gctatatcag
    15361 ctatcaaccc ttctttaaga atacctctat tttttatttt aaattgccta gctggtaatg
    15421 aagtaactct tcttacaatc tcttccaatc taacaccaga ctttctgtac atattgaaga
    15481 aaattggaaa agctcctctt ctttgtagtt caaaccatga catatcccca cccacactaa
    15541 acaaacaatc tgaaccaacc ataacaaatg gattatcaat tattttatca tcatgtagtt
    15601 tatctgggaa atcagctcta tacattcctc ctaaacaaaa gataagttct tcgtctccat
    15661 ctaaaagtaa atcgagtaat aacttgtcct catctattcc tttttgtgta gctatttcac
    15721 gtattgattt cttttccata ttaatatctt tagtgtttag aagtattata ctccccctat
    15781 ctccaagtat aaatgcttct tttaatataa gtgttcttcc ctcttcattt ctaagagcat
    15841 ccattacccc atctatacct aattcaaata gactagatga tattgccata ataaattgaa
    15901 ttactctttt tttcttcata caatgcccag aacttcttgg tactgtgtca accattattt
    15961 caacaccatt atacctagcc tttaatatct catcaaatgc ttctatttca actggactta
    16021 agtgagatac ttgaacccta gcaccacttt tctgcccaac ctctatggct tcttttactg
    16081 cattatatct tcctatgtaa tctctaatat gagatgtata tataccatca tgctctttaa
    16141 tctgccttgc aatatcaaca agttcatcaa tatcagcata cttacttggt atatatgcca
    16201 atccagtaga tattccaaaa gcaccacttt tcattccatc atttattata tctgtaattt
    16261 catttttttc cttttctgta ggaggtctat ccttagaacc tcccataaca ctccatctta
    16321 tagtcccatg ccccaacaag aatcccatat tgacagaaat accactttta gatataaggt
    16381 cacaatattc tgtaaaatta ttccaacatt tatttttatc tataagatac tttttttctt
    16441 cttcaagtaa tagaccattt ttatacatat attcatatac attttcagaa gaatatggtg
    16501 ttatagaatg tccacaatta ccatttatag ttgttgtaac tccttgtttt agaaactttt
    16561 caaatttacc atctagtatt gctactattt cttcatgaac atgagggtca ataaatccag
    16621 gtgttataat ttttccataa caatcaatct ctttatttgt attgctattt atcttagaat
    16681 ctatttttgc tattaagttg tctttaattg ctatatctcc ataaaaacca ggattacctg
    16741 taccatctat tataaatcca ttttttaaga ttaaatcata tatcaaaata ttccctcctt
    16801 aaaaaccaat ttttaaataa tcttaatgta atttttatat ttgaaaatca aattattaaa
    16861 tttcatattt gaaaattcac tattatttca ttgtaattag tcactaagct tatctttata
    16921 catagctctt gcaattaata tagtaccaaa tacacatata agaactacta gagactgtgg
    16981 tattatacct tgctttgata atataatagt caatacagat attacaattg ctactagacc
    17041 taatttttta tcttgtaaaa atacttgtcc aaaaactgac ccaaatagag ctggtaaaat
    17101 taagtttaat ttttctacta cttcagctgg aattttagat agtacgcttc ctcctaaaat
    17161 tacccctata gttagtatac ttatattcac taaaatagat acagctattc ctattgtaga
    17221 tattatcgag ccttcttctg tacctgattc aacttctgct gccttttgag ctgctattga
    17281 acatggtagc ctcatattag atatattacc agaaagaaaa ctcatataag ttcctgggat
    17341 acctaagatt ggtgaataag aaactggttc tattatgtaa tttggagcac tcatactgac
    17401 aattgccatt gtacttgcta ataatgctga tattggaggc ataataccaa atccaaagct
    17461 caaaattatt ccaggaacta atgctgctat aatacccaaa gatagtgtta gtcttccata
    17521 ccttatcata aaaggtaaat attcttcatt gtatatagat tttactttac tcataaaact
    17581 ccccccttat attactgaag ctattatcat tccgctaaac attgatattg taagccccca
    17641 ctctcttaac cattttatat ttttcttctt ttcaagcata cacaatatta ccattatagc
    17701 cattccactt atacatgcaa ttgttccatt atcaaaacgc aacaccctat cagcatttag
    17761 atatgcaaat gaacccaaca ttgctcctag agatattatt ggtataaatg atttcttgcc
    17821 actagatata acattggtta tcttatctag tttatgtcca aatagtaatg tgaatattat
    17881 ccatcctaaa gaacctaata tcatagtcca aactgcattt gtaaatgcaa gttttgtcat
    17941 ttcaggagaa cctaaagtta tacctaatgc atctgttcca aatccggctg acattgattc
    18001 aaaaattaca gaacctataa atgataatct catccatgct ataggcccac ccatagttac
    18061 tataagcgat accattccag caaatatagt cattgaaggc ccaatagctg aaatagcact
    18121 actcttaaca gctgacttta tttgtttatc tgttattccc attgttttac caactttgca
    18181 agattttata gcaaatataa gggattgtac cactactatt gatactgcta ctgtactcgc
    18241 tatccacaaa agtggatgat tagctaattt taaataatcc atttataaaa cctccctata
    18301 aatcctcaaa ttttattaat atttctaata tatctatttc caaacctgtt ttagtactct
    18361 aagtatattt ccacctagta ccttctctat ttcctcatca ctatatccat gctttactaa
    18421 atatctaaca atgttttttg aagtttctgt tgggttctca agacctttta catattctac
    18481 tttgggatgt tcttttgttc ctgttatttt ttttatatca aatttagcag ctagtactga
    18541 gtgtaatttt acatggtcgc catatactgt atctggtcca aaagctacat ggtctattcc
    18601 tactaaattt ttaatatact caaaatgttc cataaatgac tctattgaat gttctttatt
    18661 cttctctgtt attgtagtat gaggtgctgc ttctacacct ataacccctc cttttttagc
    18721 acatgctatt aatacttcat ctggagttag tctctttgtg ttccataaac ttcttgcccc
    18781 aacatggctt aatattattg ggtccttgct atattctatt acatcaagtg tagtttgaac
    18841 tccaacgtga gagcaatcta ttgccattcc aattttattc attctttcaa cagctttttt
    18901 tccgaaattt gtaagacctc catctttctc ttcttttagt cctgaaccta atgcattgga
    18961 ttcgctgtat gttataccca ttaaccttat accaaatcca tacagtatat ctattctatc
    19021 aagttcattt tctattggtg ctgcaccttc taatgttggt atcaatgcta ctttaccttc
    19081 acttttagct ctgtatatat cttctacttt ttcacacttt ataacaaaat cttgatgagc
    19141 taaatcacat agtctcatgc ctaaatctat taatatatca tcccatttcc accctgaatt
    19201 tgatgttatc gtacatacac catccattaa attatcaaat atagcatcat aatatcctct
    19261 tgataatgct tcataagcac atctttgttt accttcccta ttatattcaa atatatctct
    19321 ttctaggtgt tctggaaata atactggatg ttcatgtaat gatataaata ttttttcatc
    19381 cactatactt ttaaccttag cttcttgaat atcatctaat tcaataaggt attctttaac
    19441 tcttttatct cctttacaca tctcaataga tgaaatatct tttccttttt ctaaataact
    19501 ataggcttca tatcctttgt aagctgactt cataacaaca tcctccaagt tctacaattt
    19561 tttatatttt tcctatgtag caattgatgt gccaaaatat aaattttaat tgtataataa
    19621 atcttgcaaa aaataaaaac agtagtaagg ttgatattta ctactgtttt attaaaaata
    19681 atcttcttag attatttatt caatattttt gaattatctt aatcttaaag ataattttat
    19741 ttatttctat ccattatagg gattttttta gggtgttttg cctttttagt cccctatatc
    19801 actcccttaa tttttacata tgatataaag ttctttcctt catcagttat aaaactacct
    19861 tgttttttta ctccagataa aagtaaacca tggctcttta acttatctat tctttttcta
    19921 atttggtcta cacttaaaac tatatctttt tcttttaaaa tctcttgcag tttatttctt
    19981 cctaataata tatttttatt attccaagtt tctatactgg tcaatataca tatactttcc
    20041 tcaaagaaat tagattgttt gaaatctaag ataattgaat cgaaattttc attaactaaa
    20101 gtattgtttt tttcaaatct aaattgttct ggcaaatgct cgtaatctac tctatcttcc
    20161 tcaactatag tatcaatata ataaattaaa tttttaagct ctcttacatt tcctggccat
    20221 tcatacaatg ttaaagcttg cattgattcc tctgtaaaac atttgttgct atttatttca
    20281 tctaaaaaat acttacttat aagaggtata tcttccttac gttctctaag tctaggtatc
    20341 tctatatgta atacattaat cctataatac aaatcttctc taaaagaacc ttctttaatt
    20401 tttttcttta aatctttatt tgttgcagct ataattctaa catctattgg tataatttta
    20461 cttccgccca ttctcataac ttctttttcc tgtaatactc ttagcaatct ctgctgtaca
    20521 tctaaagata tatcacctat ctcatctaaa aaaattgttc ctgtatgtgc tctttcaaat
    20581 atacccattt ttccaccttt tattgctcct gtaaaacttc cttcatcata gccaaaaagt
    20641 tcactttcaa taagtgtatc tgacaaagaa gatagattaa ctgcaacaaa tggcttgttt
    20701 tttcttagag attcattatg tattgcctgg gcaaatattt cttttcctgt tccattttct
    20761 ccaagtatca acacagaaaa gtctgtagtt gctatttttc ttgcaatatt aattttttct
    20821 tttattattt tactctcacc tacaacactc tcaaatgtat actttgatac aaaacctttt
    20881 gactgaagtt tattttgaat cttgtcctca agcacttgaa ttgcacttat atttttaatg
    20941 cttataatac tctttattcg ttcattattt tcataaacat ttactttatt aattatcagt
    21001 tttttgttat ttaaatttac tacttcatca ttaacttcat cttcttgaaa aaatattttt
    21061 ttgacaacct tatcactaaa taagtccata aagttattag atataatttc attttggtct
    21121 attccaacta gattagaaaa tactttgttg caatatataa attttcctaa tttatcaaca
    21181 gaagcaatac cttcatctat aatctccaaa aaactcttca tggttttatt catagttgta
    21241 taatacctat atccacttac tgtatcttca ttgtattttt ccttaataat atgtagttta
    21301 tctatagata tatttagttt tgtaaagatt tctataactg tatttatatc aattacctta
    21361 tcacctatgt caattatttg ctttatgttt tgtggtatgc tgtttctgct tcccgtaata
    21421 attccaatct cacatttaga cttatcacaa cctggataat atggtattag attaatatga
    21481 attaatccta gttttctaat tatcttggca gtttcatctg ctgactcttt ataagcatct
    21541 ataaccataa catcagaatc attttctata cttattatct gatttatatt ttcaatattt
    21601 atagtacgat gtactattac aataggtata tttttatcaa tattattatg tatatattcc
    21661 tttatatcat tgtctgaaca tactacaagc tcatagcttg acagttcaat atttatccta
    21721 tttacatata ccaagttatc tatattacaa aattctccaa atatattttc caattgtttt
    21781 gatatatcta tatttatttt gtcatcagtt gatacaaata aaatattatg ttttttcata
    21841 gtcccacctc ctgtttaacg agtatagaaa gtaataattt ggtctattgt agcacttttt
    21901 aaaaaagttt taaatcttaa aaatcaaatt ttgtaccatt tactattatt ttccataata
    21961 aaataatgta agctatcttt tttcacatat aatttaattt ctaaaacggt tagtttgaca
    22021 ttattggaat ataaataaaa atttaatata aattcgttaa tatattaata catttttaag
    22081 aatgacaagg aataaattat aacaaatttt aggaatatat aataataggg tctcagaact
    22141 tagttacaag ttctaaaacc ctatttgttt agtgtaaaaa acatcataaa atttagctta
    22201 aatcttttca cataatatta ctctttttct aagtgataat aaaatatatc tattcctcta
    22261 aaaactgtaa aaattcctga ttaaatagtt taagctcatc atagaatata gcatgaccac
    22321 tatactcaaa agtatataaa attgaatctt ctattttttc attcataaaa actgcaaatt
    22381 catacggaca tatttcatct aatttcccat gaaatatacc agtaggcact cttacacatt
    22441 ttaaatcatt aaataattct tcatctctta aagatacagc agttccaata gtgccaatac
    22501 ctgatgcact aaaaccaata tcattaaacc atcttctaaa gctttctggt ggatttgatg
    22561 caaatacttt ttcgccaaaa tcggttacca tttctggtct atcagtacat gcttgtgcta
    22621 tcaatttatt tacatcttca cgtgtcatac catatggaaa ttctggaggt ctttgtacaa
    22681 atgatggtgc agcagctgca gctaatacca attttgacac tccatatcca ttaaataaac
    22741 tcatatacct aagtacgatt gctcctccca ttgaaaaacc aacaagggta aagtctttta
    22801 atccaatagc atgtaccacc ttataaatat cgtctgctaa ttggctataa gtatatcctc
    22861 ctgatgttgc atcagattta ccaaaacctc ttaaatcaat tgaaactgtt ctatatccga
    22921 gttttggcaa tatatttgtt tgatattcaa atatcttatg tcctaagggc caaccatgta
    22981 taaaaagtac agttttttta gcactcggat tcaaatcata tactgctatg tttatatcat
    23041 tcacatatac ataatacata tcaacagcaa ctcccttcaa gaaaatgtat gatggttatt
    23101 ttatatatat gaataaaatg atgaataact aaattttcaa atttatttac aatacttctc
    23161 tattgccttt tcaaataaac ttacatgaag tttttcatct aggattattc tatttaatat
    23221 agcttttata taagggtctt caataagact tatatcttta taataattat taatagccac
    23281 atgctccaaa tgcaaatcta gttttaatct gttacaaata gaatttccat aacatacaaa
    23341 accaccattc caatatgcac cacaagaact tgtagaacct ctatatacag gatttccacc
    23401 caataatctt atagtctttg ctaatatctc catatgtaac atttctgtta tagatatatt
    23461 tatccacatt tcacttaact ctctatgcgt ttcatctaaa tcaaaattat ggtatagata
    23521 ttgatttaca ctcgtaaatt cacttgatac tccagcatag tcatccatca ataattctgc
    23581 ataatactta tttggaccta aaacttttat ctctggatat ggctcatcac ttgaatatcc
    23641 atttcgcttg tgcatgtcat aatgtgcacc actcataata tctcctcctt taaatttaat
    23701 gtatgatatt aattgtatgc atcacaactt tttcatataa cttagataat aaaatctact
    23761 taaataataa aaagccatat taattaaaat taacatgacc ttttatccac aaaattactt
    23821 tttaattatt catcttttcc agctactgat agctctttaa taacaactga aggtgaacct
    23881 atactactca ttggaaattc taaatcttct cctatagctt caatatcttt caacaggtca
    23941 aaatagtttc ctgctactgt aatttgctcc actggaaata ctttttttcc ttctttgata
    24001 taaaagccct tggcagctaa agaaaagtct cctgttactg aatttgcacc tgaatgaagt
    24061 cctgcaaagc tagtaaccat caatccttca tctatatcct ttataatttc atctaaagat
    24121 ttatctcctt tctctatata aaaattagtt ggatatatgc ttataggtga tgaatatgaa
    24181 gattttattc catttcctgt agtctttaca cctgccttat tagccgtttt taagttatga
    24241 agtaaagtta taagctttcc atttaaaaca acttcttttt taaatgtagc tactccttca
    24301 tcatcaaaag gagtagatgc cataccattt tctaaaagtg ggtcatcaac tattgtaact
    24361 ataggagatg ctatcatgtc tccttctcta tttttcaata aagataatcc tttttgagct
    24421 gaatctgcat taaatatccc agaaaatgtg ctaagcaaag ataccatagc ttcattaagc
    24481 aatatagttt tatatttacc tgatgtaata ctcttaccac ctactttaga taaagcatta
    24541 tcaacaccaa gttttgctat ctcacaaggt tttatttctt caatagaatt tgctatatta
    24601 tatcctattc catcatattt ttgtccattg tcttctatga taggaactac aaaccctata
    24661 agcatattag ttttattgct taaattaaga cctttagtat tagatatacc attacttgat
    24721 acagaataag atatagtaca tttagataaa tttaccacct tatctgaata agctttagtc
    24781 tctctttcca tttctaaagc taaatcaatc aacttatctg cctctaaatt ttctagctct
    24841 ttagaataag tctttacatc attatagtgc ttatctcctt catatataaa ttgtacatct
    24901 tcattttcaa tagtgtttac tccatctttt acatttttta tcaacatatc aacagcttta
    24961 tcatctaaaa tttctgtata agaataacct atctttccat ttaccttacc tctaaaagat
    25021 aaaccaaaag atttatcaag attgtatttc tcaacttctc cttcatatat atttatactt
    25081 aaattttctc ctgtataata atatatttca cactcttcaa aaccttcact tagagccttt
    25141 ttaaataata tatctttaaa atttttaagt tccacaatta tccctcctat cttccaccta
    25201 cagtaatttc ttttaccctt atcataggtt ggcctacatt agttggtata cttcctgatg
    25261 atgagccaca catcccctga gcttgtttta cgttattccc aaccatatct atattcataa
    25321 gtatatcgct accttttcca attaaactag cacctctaac tggctcttgt atttctccat
    25381 tttttattaa gtaaccttct gaaactgcaa aattaaactc tcctgttaca ggatttacag
    25441 aaccaccacc cattttttta gcataaagtc catctggtat tgactttata atatcttctg
    25501 gtctatcttc tccagcagca atataagtgt tagtcatcct tgaagttggt gcaaatttgt
    25561 agttttgtct acgagaactt cctgtaggat tcattcccat tcgtctacca ttaagtttat
    25621 ctatcatata agacttcaaa attccacttt caatcagtat attcttttga gttggatttc
    25681 cctcatcatc tatattcata gaaccccagt agtttggtat tgtaccatca tctatagctg
    25741 taatctttgt ggaagcaatc tgttgaccta atttatctgc aaatacagag ttcccctttg
    25801 caactgatgt agcttccaaa gaatgtccac aagcttcatg aaatattact ccaccaaatc
    25861 cattatctat tgctaccatc atattacctg caggacaatt tttagcatgc aacatagtat
    25921 gcgctactct agctgattct tttgcataat attctgggtc tatttcttca aatatttcaa
    25981 accctttaca acctccagga ccttcaaatc cagtttgatt ttcattaccc ttagatgcaa
    26041 ttgaacttat ccctagtcta gttcttattc ttctatcctc aacatatata ccttcagtgt
    26101 ttgcaattaa tattttttgg tctttatcca aataactgac acttacttga gatatatcat
    26161 tactaaattc ttttgcactc ttatatgcaa ccttcataac acttacttta tctttatttc
    26221 ctattgaatt tggataaatt tttatattat gtatgttatt tattttaata ctgtcattta
    26281 atattacact ttttccatct tccaatttac ctaaagcaac agcagctttg tatgctacat
    26341 ccaacagact atttaatgaa ttgtcacttg tataagcata tatacttttc aatcctttaa
    26401 atattcttat tcctatacca taatttcttc cacctattgc attttctact ttattatcta
    26461 ttaaactaat agaattattt atagtgtctt cttcaaaaat ctcagcaaaa tctcctccag
    26521 taattagaca tttttctaat accttagaag ctatattttt tgaaagcata aatttacctc
    26581 ctactttaaa tttttaatta acttttattc aatcttttta cttgttaaca aacattatat
    26641 actatattac ttaaaataag gtaattatga atatattata catatgtttt atactaaaat
    26701 gttatcacat tttatctata catacccata taactagata attaaaatgt ataatattat
    26761 ttgctataga aaataataca ctcatacata ataaatttta ggagtgaagt atatgaatca
    26821 agaatatgta aaaggaaatc ctatttttac taatattaat aaagttccaa gacaatttcc
    26881 ataccttact gatgatattg atactgatgt tataattgtt ggtggtggcg taactggatg
    26941 tatttgtgct tactatctag ctaaaaacaa tataaagtct gtcattcttg aaaaaggaag
    27001 gattgcacat ggaagcacta gcgtgacaac ttctctatta cagtatgaat tagatgacaa
    27061 tttgatagat ttaacagagg ttatgactct aaaagatgct ctaaaagcat acaacctttg
    27121 tacttcagca cttgaagaat tagatacatt tatagagcta tatggaaata agtgtgatta
    27181 tgccaaaaga gatactctac tttatactgc caacaaactt gaagttaagg ctatcaaaga
    27241 agaatataac ttaagaaaag aaaatggatt tgatgttgaa tatattgatg aatccacaaa
    27301 cccttttagc tttgatttaa aatcaggatt gatagcaaag aatggaggac gagaattaga
    27361 cccatataaa tatagtcacc atttgattga tgtaagttta aaaaatggtc ttcaagtata
    27421 tgaaaataca gaagtaaaaa aagtagattt atcaaatgat aaggttactg ctgaagtatc
    27481 gtatggatac aaagttcatg gtaaaaaatt aattgtagct acagggtaca atacaagttt
    27541 atttacaaaa agaaattttg caactaagtc aaatactttt aatatagcaa cgaaaccact
    27601 taaaaacata gcttcttgga aaaacaacat tttaattaga gataactgtg acccatataa
    27661 ttatctaaga actactaaag acaatcgatt aattataggt ggtgaagatg taagttttga
    27721 tgatatagaa aatgaaactt tagccaatga aaagtatgat attttagaac aaagattgaa
    27781 aagtatgttt aaggatataa aagacattga agttgaatat aaatattgtg gttgttttgc
    27841 ttcaacttta gataatttag gttttattgg tccagataat aaaaataaca atctatggta
    27901 ttgcttaggt tatggagcaa atggtatact ctttgcaata ttaggaggta taatgttaag
    27961 tgaattatat cttggcaaac aaaataaaaa tatgaaatta tttaaagtag atagatttga
    28021 taaatagata ttatatatta aaagactgta aaaagatgaa actttataat tctaaaagtt
    28081 aactttgata cagtctttta taatgttcct tttaaaaata ctttatttat aattaaatat
    28141 aaagtaaaat gttaaaatat aactaataaa atttagtcat aagataatat ttaagtttat
    28201 ttgatgaaat atggaggaat tattatgaag gaaatactcg ttattgctcc cactaaagga
    28261 acctatgaaa aatcaattca catagttaaa aaaaacaaat atacaaatat tgatgttgta
    28321 tttggcaatc taaaagaagg tatacctctt gctgaaaaaa gtattaatca tggtactaga
    28381 attatcataa gccgaggtgg aacttacaat atgttaaaag ctacttacaa tatacctata
    28441 gttgaaatta aagtagatgc ttatgatatt ataaaaagct acaaggaggt aaaaaattcc
    28501 aacgaaccat ttggaataat tggatttaat aatgtcatct acggtttcga tattatagaa
    28561 gaaatattga ataagaaaat aactatgatt gaaattgaaa aagaagaaga aatttatgat
    28621 gctatagaaa agtacagaaa gaaaggtata aacacatata taggagatac cactgttgca
    28681 catattgtta agagacttaa ttgtaaggga atattaatcg aatctagaga agaaaacata
    28741 cttagagcca ttcaacaagc agaacaaata ctcgaagcaa ccaaggatga acaaaaaaga
    28801 agattacaga tagaagctat gacagatttt gtacatgatg gaataataac agttgataaa
    28861 aattttaaaa taacattatt taataagagt gctgaaaaaa tatttggtat aaaaaaagaa
    28921 aatgcacttt atcataatgt tgtagatgtt atcccaaaca cagttcttcc acaagtagtt
    28981 gagactatgg aacctcaaat tggtgaaata caaaatatag ggagtacaaa aattattaca
    29041 aatagagttc caataattgt tgatggtcag attcaagggg ctgtttcaac atttcaagat
    29101 agcaaggaga taagttcttt tgaacataca ataagaagga gcttattaag caaaggtcta
    29161 tctgctaaat acacttttga tgatataatt catgctagtg aaaagacaaa agattgtata
    29221 gaacttgcaa aaaaatatgc ttgttatgat actcctatga ttattactgg agaatctgga
    29281 gttggtaaag aactattttg tcaaagtata cataactata gtaagcgtaa aaatgcacct
    29341 tttgttgctg taaattgtgc tgccatacct ccatctctaa tagagagtga gttttttggt
    29401 tatgaggaag gttcatttac aggtgctaga aaaaaaggta aagcaggtgt attcgaacta
    29461 gcacatgaag gcacaatctt cctagatgaa ataagtgaaa tccctcttga acttcaaggt
    29521 cgattattga gggtattgca ggaaaaacaa gttatgagaa ttggtggtga taaagttatt
    29581 cctatagatg taaaaattat ctgtgcttca aataaagact taaaaaatat gatgcgcaat
    29641 ggtttattta gacaagacct ttattttaga ataaatatac taacattata tcttccttca
    29701 ttaaaagaaa gaaaagaaga tattataaaa ttatcagaat tttttataca taaatattca
    29761 agcaaataca ataaaacgcc tttaattatc actccaaata ttaaaaaggc acttcttgaa
    29821 cgagaatatg aaggtaatat aagggaattg gaaggtatga tggaaagggc tgttattgtg
    29881 gaatcatttg actcgatttt attagaaaag cttagcaatg aatatacaaa taatatagtt
    29941 gataaagata atagtgattt tgaatgcagt aatttagatt taaaaacttt agaaaaaaaa
    30001 tatatctata aaatttataa cgaaaataat agaaatacta caaaaacctg tgaaattcta
    30061 aagataactc gctccactct atggcgtaag ttaaaagaaa tagaagatga tgtttaaaaa
    30121 taatacatat ctatatatat caaaacgttt tatgtttaat tttaatacat aagacgttta
    30181 tttgttttca ataaaaaata caattatatg ctaaaaagaa tctttgtaag tgaaattctc
    30241 gtcaatcata cttttaatta taagtaatct acagcatttc aatcgattat taaaaataat
    30301 catgataact aaagatacaa actctatata ttggcatagt aattgctttt ataataccta
    30361 tataaaaatg aaagggagtg atactagatg cacatcttaa aatcagtgaa aaagatacca
    30421 ggaggactta tggtctgtcc cttgttattg ggagcactat ttaatacagt atgtccacaa
    30481 gctcttgaaa ttggaggatt cacaacatcc ttatttaaaa ctggtgcaat gagtatactt
    30541 gctctatttt gtctatgcaa tggagctcaa atcaatatca gacaagctgg taaaccattg
    30601 actaaaggta tagtacttac agcaacaaaa tttataattg gtgcagtatt aggtattatt
    30661 gttagtaaat ttattggacc taaaggtata attggtatta cacctcttgc catagttgct
    30721 accatgacta attcaaatgg aggtctattt gctgctcttg caggtgaata tggagactcc
    30781 acagatgttg gagcaatatc tatcttatct ctaaatgatg gtcctttttt tacaatgctt
    30841 gcttttggag taactggact tgcaaatgta cctatagtag cactatttgc agcacttata
    30901 ccaatactac ttggatttat acttggtaac cttgatgaag atatacgaga atttcttgca
    30961 cctggtacaa cattattaat tcctttcttc gcttttcctt taggagcagc attaaacttt
    31021 aatcaaatta taactgctgg actacctgga gttgtacttg gtttaggagt tactataata
    31081 acaggtatgg gtgggtactt tgtaatgaaa cttatacgtg cagaacatcc agcagtaggt
    31141 gcagctacag gtacaacagc cggaaatgca gctggtactc ctgaagcttt agcagctata
    31201 gaccctactc tagctacaat agcagctgtt tcaacagtac aagttgctgc tgctattatt
    31261 gttacagcaa tatgttgtcc aatgttagtt tcctatctag ataaaagaga aaaaaagaaa
    31321 aaatctattt ttaaaacaaa taaaaatatt gaagtaaatt aatacttaag gaggtaaaat
    31381 taatgaatgc aattattcat gcaaatgaaa aagataatct agttacatgt gtcagacctt
    31441 taattaaagg tgaaaaagtt tcaattgatg gaaagtctta catagtaaat gataatatac
    31501 ctgtctttca taagatggct acagaagatg taaaaaaagg tgatgttgta tacaaatacg
    31561 gtgaaattat aggaattgca acagttgaca taaaaactgg tgactatgtt catgtacata
    31621 acattgaaag tacacgtggt cgtggagata aaaaatagga ggaagttaaa atgaaattaa
    31681 tgggttattt aagagaaaat ggtcagtttg gtattcgtaa tcatgtttta attattccaa
    31741 catctgtatg ttcaagtgag acagctacaa gaatagcatc acttgtgcct ggagcaatag
    31801 caatccctca tcaacatgga tgttgtcaaa ttggttctga tatagagctt acagcaaaga
    31861 cattaatagg atttggaaaa aatcctaatg ttgccgcagt tttagtagtt ggtcttggat
    31921 gtgatggtat tcaagcaaaa gaattagcat ctgaaatagc tacaacagga aaaaaggtaa
    31981 attacgttgt tatacaagaa tgtggtggaa cattaaaaac agtttctaag ggagctgaaa
    32041 tagtaagtaa aatggctcgt gaagtatcaa aagaagtaag agttgaattt ggtatgagtg
    32101 aaattacatt agcacttgaa tgtggtggtt ctgacccaac aagtggaata gcttcaaacc
    32161 cttcaatagg cgttgcatca aaccttcttg tagatgaagg tggaagtagc atactaagtg
    32221 aaactacaga agtaattggt gcagaacatt tgttggctac ccgttttgaa gatgaagaaa
    32281 tgaaagataa gtttcttaaa tttgtaagtg atgtagaaaa aagagcaatt gctatgggag
    32341 aagatttacg tagtggtcaa cctactccag gcaataaagc tggtggactt tcaacaattg
    32401 aagaaaagtc acttgggtgt atgtataagg caggcaataa gccattcaaa ggtgctcttg
    32461 aatatgctga tattgtacct cctgataaaa aaggattata ttttatggat actccaggtc
    32521 aagatataga ttctatcact ggtatggtag caggtggtgc acaaattgta atcttttcaa
    32581 ctggtagagg tactccaaca ggaagcccta tttcaccagt tattaaaatc actgggaata
    32641 gtgatacata caacaaaatg ccagacaata tcgatataaa tgcaggtaga attataacag
    32701 atggtgctaa aatagcagat ataggtcaag aaatatttaa tgaaataata gaagtctgta
    32761 atggtaaaca taccaaagct gaaagtcttg gtcatagaga atttggcatt tacagaattt
    32821 catctacatt ttagattgta acaaaatatc ttttataaat ataatactta tgtagataca
    32881 taagctaaaa gttctttcta atactataaa aattttctat actgaaaagg aactgtatca
    32941 aaattatttt gaaaacagtt ccttttttaa cctcttttac tcaaaatatt ataaaataag
    33001 atttaaacaa taaacgtatc ttgattaatc tctaagaaga cataaataaa gctaaatgta
    33061 aagtgggata aataaaattt aatatctttg tatgaaaaga tatttggtat aatatgtctg
    33121 agagaattct tatttgtaaa caaataaagg atggaggatg aaacaaaaga atgaactttg
    33181 aaaacacaaa aaataaaaaa ataatagcta ctttactatt gacactcctt ttaatcgtgt
    33241 cattaacatg tgtcactttt attatgaatc taaataaagt aacacataat gaaacagaac
    33301 gatatcttgt agaagtttca caaagtattt ccactacaat taatgtgaaa tgtaaaagta
    33361 tatttaggac actatattct atgggagata cttattttga gattaatgat agtgaaaaag
    33421 aagtcaatag atacttatca aaaaaattaa aattatttga atttgattgg ttaggtatta
    33481 tagattctaa tggaaatttt gtaagtgccc ttaaagatac aggtaatgtt aaaggacaag
    33541 acttttatag aaaagccctt caaggcaaag aaacaattac tcctgttaaa tcacaatctg
    33601 gtacatatgg aattttatat tctgtaccac tatacaatgg tgataaaatt gttggagctg
    33661 ttgctgcttg gaatactatt gatactatga gaaatttatt aagtgtagaa agctttcatg
    33721 gtgaaggatt ttcccaaatt atcgaccaag atggaaattt tattgtaagt tcagcaaaca
    33781 aaaatgctcc aaaaaatgtg gtaaattttt ttgacatgat taaacaccaa ggaaaacagg
    33841 ttgataaaaa taaacttgaa gaaatgaaaa taaacttaaa aaagaacaaa actggttcta
    33901 tgtattatac attaaatgat ggtatttcta aggcaatgca ttatcttcca ttacaagaag
    33961 aaaacttata cctcctttca attgttccaa caaaaacagc aagtgcacag tttgatttac
    34021 ttctaaaaaa agcaatcttc atcaatattt taattgttat actattttta ttgcttatta
    34081 tattgattat gtttataaat caaaagaaca gggcacaact tataaaaatc gcctatgtag
    34141 actctgtaac taaaggttac agtaaagcac gttttgatat tgaagctaaa aaaattatac
    34201 agtcatcttc atcagaaagc tattcattgg tatctataaa tattcagaaa ttcaaactta
    34261 ttaatgattc ttttggtagt gaagcaggag ataaaacatt aaagtacata catgatgtca
    34321 ttagtaatta tctagaacca ggagaattgc tttgtcgagt ttctaatgac cagtttaatg
    34381 tacttattaa aactattcct caaaaagaaa ttcttaagta catagaaaat atcaccaatg
    34441 atattaatag tttcaataaa aatgaagaat gtaaatattt tatagctcta tctataggtg
    34501 tatataaaat tgatgaccca aatctacctt tagtttcaat tcaagaccgt gcaaatgttg
    34561 cacgaaaaaa taataaagga aacattggaa atcatccact ttatacatgt gttttttata
    34621 gtgatttaga gcgtttaaaa atgattaaag aaaaagaaat ggaaaataga atggaggaag
    34681 cattaaataa taacgaattt atagtatatc ttcagcctaa aataagcctc aaagataata
    34741 ctattgtagg tgcagaagca ttagtaagat ggcaagaccc aaataaaggt cttataccac
    34801 cagacgagtt tattcctttc tttgaaaaaa atggttttat tacaaaactc gaccactatg
    34861 tttttgaaaa agtatgcagt atgataagaa aatggataga taatggagaa actccaatac
    34921 ctgtttcagt taatttttca cgtgctaatt taaataatat agatttttta gaaaaataca
    34981 gaatgcttcg taataaatac aacattcctg ctaagctctt agaaattgaa ttaactgaaa
    35041 cacttgtatt tgaaaatctc caaaaattaa ttaatataat agaccaaatc catgaagaag
    35101 gttttcaatg ttcactagat gactttggaa gtggatactc ttcactcaat atgttaaaag
    35161 aaataaaagt tgacacatta aaacttgacc gtgctttttt tagctcacct aatgcagata
    35221 atattgctga aaatcatgta atagaatcta tagttaattt ggcaaaaaaa ttaaatatga
    35281 atagtatatc agaaggtgtt gaaacaatct tgcaaatgga ttatcttaaa aaaattaatt
    35341 gtgatatgat acaaggatat gttttttcaa aacctctacc tcaagaaata tttgagaaaa
    35401 tgacttttgg aaaaaaacta gaagaaacca gtatggatag ttccgtttaa ataaaataaa
    35461 aaacttgtct ctataaaata attttaatca ttttataaag acaagtcttt ttattttcca
    35521 atttaatttt attttctaaa tttcttacac tcggcttgtt ttaaattccc tttttaagaa
    35581 taataataca attatcatag caataaaatc tgcaactggc tgtgccaacc aaacaccatc
    35641 taaattaaat tgaattggaa gtacaatgat taatggtata aataaaataa attgtcttaa
    35701 aagagttaaa aacattgaat gtttaacttt acttatacac tgaaaataca caggtcctac
    35761 aatagaaatc cccattatag gcaatgtaat tagatttagg cttatacctc ttactgctat
    35821 atccatcaaa tctgaatctt tattaaatat acctacaaat acttctggga aaacttgtac
    35881 tactaaaaat ccaaatgaca atatgacaac tgacactatg atagataata ttaaagtttt
    35941 ctttgcacgc tcatattgtt ttgcaccata attgtatgat attatagttt gcataccttg
    36001 attcaatcca aacacaggca tcataaacat caataaaata gatgtaagtg ctgtcatagc
    36061 tccaatagct aaatctccac cataaacctt tagagaatta tttgtaaaca tgtgagttat
    36121 acttatagct acttccatag caaaaggtgt cattcctatt gcaaatacct tctttactat
    36181 atctttattc aattttaagt tacatttttt tagttttaaa ttggacttcc ctcttataaa
    36241 ataatgtatt acccaaaaga atactactat ttgacagagt actgttgcta tagctgctcc
    36301 ttttatacct aaattaaata caaatataaa tatagggtcc aatatcaggt ttaatacaca
    36361 tcctacaatc attgtccttg ctgctaattt tggattacct tctgccctaa ttgcattatt
    36421 aagagaaaat gcaactaaat taaatatact tccaaccaat atgacactca tatagtcttt
    36481 tgcataaaat attgaatctt tacttgcacc caaaataaaa agtatatctt ctaagaaaac
    36541 taatcctaat atagttataa ttaaagagat aattatagat agtgtcaaag cattacctaa
    36601 tatcttctct gcttcttctc tatttctttc ccctaacttt atagacaatc ttgtgctagc
    36661 acctactgat accaacattc caaaagctat tattagagta aatactggca tagttacacc
    36721 aagacctgca atagctatag agccaatacc ttccatagaa ccaataaaag ctctatctac
    36781 tacattataa agtgaagtta ccatcattgc tattattgca ggtatcgaat acctaaataa
    36841 taaaatccat attttttcat gtcttaatgc ttcttgattc tccatgttgt caccatttac
    36901 accatgtcat aagttataca aattggttta tttgttcttg ggtctgttgc tatttctgca
    36961 tcaatattaa ataattcttt tagattatcc ttcgtcataa catcatatgc atcaccttca
    37021 caaactattt tacctttctt aatacctatc atgtggtcag caaatcttgc tgcattatta
    37081 agttcatgta ttaccatgac aatagttctt ccttgttttt tatttagttc atctaataat
    37141 tttagtatct ccaattgatg tgccaaatct aaatatgttg taggctcatc cagtaataat
    37201 atatcagttt cttgtgccaa agccatagct atccatgccc tttgtctttg tcctcctgat
    37261 aaatcttcca tatttctatc cttaaattct gcaattccag taacttctaa actccaattt
    37321 acaatctctc tgtcttcttt actagaatta ccaaatccac ttttatgagg aaatcttcca
    37381 taagaaatta attctgaaac ggtaagacca ctaggagctt gagggctttg tggaagtaca
    37441 gccatatctt ttgctatttc tttagacttc tttttgtaga tgtcctctcc atttatatat
    37501 attttcccac tattagggct aataattcta cctatagttt taagtattgt cgattttcca
    37561 caaccattag cccctatgat ggtagttatt tttccttttg gtatatttaa atttaaattt
    37621 tttataatat ctgtgtttcc atatgataca tttagattct tagtatttat acagttcata
    37681 acaacaccct ttcctaatca tttaccaaca ttaaataaat aaagtacggc actcctatta
    37741 tagaaactac tatacctact ggtatttcta taggtgctag aagatttctt gatatagtat
    37801 ctgctattaa aagtaataat gttcctatca atgctgatac tggtattaat cttttatgct
    37861 ttggtcctat tatttttcta cctatatgtg gagctattaa acctaaaaat gaaatactac
    37921 ctgctactgc tgtcgataca ccagataaaa ttactgcata tattataagt cttcttcttt
    37981 gtttttctac atctacacca agacttatag atacttcatc acccaaattt aaaacatcta
    38041 tatatttaga tttataaata gttataccca taaaaatcac tataaatgga agcacagcta
    38101 ttacatattt ccaactggtt ccccaaatgc ttcctgatgt ccatgccata accctattaa
    38161 attcctgtgt tgtaaatttc aattggaata ttgtcaaaat tgaagtaaaa gctatgttta
    38221 cacctatacc aataagtagt aatcttgatg aatttatacc acctttccat gcaagagaat
    38281 atataagaaa tgctccaaat aaagctccta taagtgcaac aataggcatt gtaaatacag
    38341 tcaagttact cataccttca tatacattac cattcataag gtatatataa actactacaa
    38401 acaatgcagc acctgaattt attcccaaaa ttccagaatc agctaaatca ttctttgtaa
    38461 ctccttgaag tattacacct gctgttgata atgcagaacc aaccaaaagt gctattacta
    38521 ttcttggtaa tcttaattta aatattgcaa tttcatgatt acgctgaccc tgccctaaaa
    38581 aagtctttat aacatccatt ggttctatag aaaatgagcc catatttaaa ctcaataaaa
    38641 aagtgataaa aataagcacc attaaaattg atataacaag taatgtttta gaatttattt
    38701 ttataatatt tttcatagta cctatttctc cccctttcta acaaggtata taaatattgg
    38761 cactcctata agtgctgtta tagaacctac tggtgtctca tatggaggat ttatcatcct
    38821 acttaatata tctgtatata caagcaatac agctcctaat actaaagagc tcggtattat
    38881 atacttataa tctgaaccta ctattccttt agctatttga ggaactataa gacctataaa
    38941 aataatatta ccagacactg acacagatgc tccagtcatt aatattacaa gtactataca
    39001 tataaatcta acaaagttag ttttttgtcc taatcctatt gccacttctt ctccaagact
    39061 taatatagtt attcttggag ccataataat agctaaaata aatccaatac caccagctac
    39121 aaatagcaat tttatacctt cccacttcac tcctacaagc cctcctgata tccagaaact
    39181 taattgttga gataggttaa aatacataga tattgctgat gccaaagaaa ttagcaatgt
    39241 accaagagcg actcctgcaa gagctaattt tacattatta actcttcctc ttgatttaga
    39301 acttataaaa tatataaata atccacttat acttgctcca ataaatgcaa acatcattac
    39361 actaaaactt ccatgtataa gttgaggaag ttttttttga agtaccagtg atatcgaaat
    39421 tgcgaatgta gctccttgtg taattcctat aacagatggt tcagctatag gatttctcgt
    39481 tattccttgc attattgccc cagaaactgc taaaaatcct ccaacaagtg cagctgctat
    39541 tgcccttgga agtctaacat cctttactat tctcatattt attccatctt ccatttttat
    39601 caaactatct ataacagttg atagattcat atcttttgcc ccaatagtta tggataatac
    39661 aattccaaat acaagtacaa ctatacttac taatatcaac aaataagtta tatttttttt
    39721 actcagctta gtcaattatc ctacctcctt ttccctttta atcgtcagta taatatcatc
    39781 tattaagtat tttaatttta agatttatta aaaataggtg tgaaaaatga atatcattat
    39841 ctcacaccta ctaagttaag aattactttg ttaattcatt tttaactgat tctaataata
    39901 actcttttcc aattggattg tatgattgac tgaaaaatgg agcagcatca agtattgtta
    39961 cattaccatc ttttactgct cttatttgag cccagatagc actattttct aaatcttttt
    40021 tatctgcttc tgtagcaatt acaactatat ggtctgcatc tatatctgtt aaaccttcca
    40081 tacttaccat tggtagtgtt attccatcct gttttggcat attcttaggt ttagctaatt
    40141 tcatatcatc attgattaga gtacctattc caccatcact aaataccata aattgacctg
    40201 aacttgccaa cactggtaaa taagtttttt ctccattctt ttctataact tccttaccta
    40261 actttgtagc tttttcatca tacttctgta gccaactttt agcttcttct tctttgtcaa
    40321 ataactttga aacatcagtt aacttgcttc tccagtcatt tgcataatct ttcatcataa
    40381 ctacaggagc tatttctttt aattggtcat atattttttc ttgtctttga ctcattatta
    40441 ttaaatcagg atttacttct aatatagctt ccatatccat agtatccatc attgagtgcc
    40501 caactatctt tacaccttta agctcttctc ttatgtaact tggtaattta tcagtttcat
    40561 atgagtctac atttgctgtt gcaacaggct tataacctgc taataatagt tcttcactac
    40621 ttcctgatat atcaactatt tttttaggat ttgatggtat cttaacctct cctttaacag
    40681 attgtacaac tcttgtctct gatttatctt tatcctctgt attcttatct gaacatgctg
    40741 taacaagtac taacgtagtt ataatagata taaatattgc aagtgatttt atttttttca
    40801 tgattacttc tcctacttgt ttattatact tacatgatat tgattatcat aagcacatat
    40861 ttattatagc atttttctat ttttagtcaa tattattttg taaaaggata aaaattccta
    40921 attaatctta attatatata gcttagataa ggttttctta tattaaacag tattttttta
    40981 aagaattgga cacaaataca atgacttttc agttaaacaa tgataaaatg caatttatag
    41041 ataatgaatt tagtaaggag gattcaaaac atggtacatt tagtatattg tgataatgca
    41101 ggaaaaaaag gagaaaaggt cttagataaa atcttggctg gaacaaaaac aatggttatt
    41161 agaggtgctg ctggtagaaa aataccacat agtagagtat ttgaagatga gttactttat
    41221 tttatggaaa aaggaactgc aaaaatatct gcaaaagcca ctgttaaaaa ggtagaaaac
    41281 tatgttaaat taacagaaga tgaaatttct aaaattttaa atgaaaatca accaaaatta
    41341 aatttatctg aaaagcaaaa agttcgttgg cataaaaaat gtttatgctt agttgaattt
    41401 gaaaatgttg aagaaataac acctcttgat tttgaccatc aaggaaatat ggatgattgg
    41461 cttattgttg aaaaaattga agatgtagtt gttggtacta gtatacctta caattatgag
    41521 aagtctaaat tttaaagtat caataacata atactatatt gctaaactct acttaaataa
    41581 aaaaagagtg catttaataa ttataaaaat tatttttata cactcttttt taataataat
    41641 tttaaattac ttactttcta atctttcata tacatctata aattcttctg ctaattgttg
    41701 aaaattcata gctgtcatta aatggtcttg tgcatgaata agtataacac ttatttcagt
    41761 tttattacca ttagcttcat tttgtatcaa ttctgtttgg aatttatgag ctttatgaat
    41821 tgattctttc gacttttcaa tacattctct agctttatct atattcccgc tctttgcttc
    41881 ttttattgct tcaaatgcta gccctttcgc atcacctgca aatccaatta ttccaaaaga
    41941 tatctctatc atcttctcat ccatttattc gacctcctaa atatctctat agcttgatat
    42001 gactaatcca ttcttttcta aaaattcttt gactttttta gatgtaagta tcttgtgctc
    42061 tttcgttcta tcaatagcat aagatgtaga atttaaaata taatcatcta aaaaagctgg
    42121 gtgagacata atatccacta catcatactt cattatttcg tctatatttt ttataaaata
    42181 ttcttcactt acattttcct tataaaaact atctattaaa ggaactactt tagaatactc
    42241 taaattatat tcaaatccac ctcttattgg aagattatac ttggttacaa tcttctctat
    42301 tactggcttt aaacttaata aagtatggat atgatggtga ctatctaaat gagatatatc
    42361 tattccaagc ccttttaccc tatctatttg agcacaaagt tctctataaa tctcatcaca
    42421 atccattttt tcaataattt cgtctgtaat cctcctaata aaaaaaccgt ctttatcaac
    42481 aatagttttt aagttagata ataatggtct accacaactt aaagtcatat gaactccaca
    42541 atttaaagtt ttattttctt taagtaagcc tactccatgt tctaccccag gcatatttgc
    42601 catcatagaa gtagacctaa ctataccatt attatgagct gaaattattc catagttcac
    42661 agcttcacaa tatccaaaat catctgcatt tattataatt ttagtcatac cataacctac
    42721 tttctataga caactatcta ttaaatctag gaagataatc tttatgagct tccaacattt
    42781 cgtctaatat agtttttgct aagtcatcag atggaatcaa tgggtttata caaagtgcca
    42841 aaagagcaga ttcataatcg ccatatacag ctgctttagc tgccaatact tcaaatgatt
    42901 tgatttgact tactaatcca tgcacagaat ctggtaaata tcctatactt attggttttg
    42961 gaccattttt tgttattaca ctacttactt ctaccgcttg ttcatcttta aaatctctta
    43021 tagaaccctt atttaatgta ttaactactt ggatatcttt tttgtcgttg tatatagaac
    43081 ttattaaatt acaagcagca tcactatagt aagcccctcc tcttttttca agttgaggtg
    43141 gttttacatc taatttttca tccttatata attcaaataa ttgttcttcc aattctttaa
    43201 ctacttgacc tctagctttt ccctctttaa actctctcaa ctcatcctca agcatttctt
    43261 tagttttata gtagtatcta tgatatggac aaggtattgc acccaaagat tttacaaatt
    43321 cagcattaaa atcaattgcc tttatatttt gcatacttat atctgctttg acaaatttat
    43381 caattgcttc ttttgtaaca tcttctccat ctaaagatac atttagacca taaaccatat
    43441 gattaagacc tgcaaaatcc atacttattc tgtcactttc tacattaaat agtttggcaa
    43501 catcattttt taaatgaatt ggcacgttgc atagacctat atatcttcta aaattagtat
    43561 atttgaatac agcctctgtt attattcctg ttggatttgt aaaattcacc atccatgcat
    43621 ttggacataa ttcttccaca tcttttatta tatcaaatat aactggtatt gtgcgaaggg
    43681 ctttaaataa acctccagca ccattagttt cctgccccat aactccatga gataaaggaa
    43741 tagtttcatc ttttattctc gcatctaaaa gacctactct aagttgtgtt gtcacaaagt
    43801 ctgcatctct tagagcttct cttctatcta aagtaagatt tattttcata tctactccag
    43861 ctttttttac catcctctta gctagatttc caactatctc taatttttct tttccagctt
    43921 ctatatctac aagccataat tcctttacag gtaactcttc atatctattt ataaaaccct
    43981 ctacaagctc aggagtataa cttgaacctc ctccaattgt aactattttt agtccctttt
    44041 tcatctcatt ataccatcct tacctataat ttaatatact ctacaaaacc ccatacattt
    44101 atttaaaact ctatacattt atttaccgaa ataaaactac ctagttgtta attttcaatt
    44161 tattagcttc cattttttca gacgctatga caaatggaat atatattaat attgataata
    44221 ttaaattaaa tagtgctaaa gctccaccag tccagtcacc tgtagccatt gctccaccta
    44281 atataggagg tgttacccaa ggtattttag gtaaaattac tggtgctact aatccaaaat
    44341 ctattgctaa gtatgataca gcactacata ttactggtgc aagtacaaat ggaatcataa
    44401 atataggatt taaaactatt ggaagtccaa atatcaatgg ttctcctatg ttaaatattg
    44461 caggtggacc acctaatact atcatctctt tacgtcttct tccagctatt aataaagcta
    44521 ctattaatcc caataccatt ccagaaccac ctaaatatac aaatgcatca aagaatgcac
    44581 ctgcaagtac atggtatcct tccgttgctc ctgcttgagc caaggcagca ttttctccac
    44641 ctaatttcat aagtatagtt tctgtaaaag gaagtgctat atttgcacca tgaagtccaa
    44701 cagtccaaag tatatgaatt ataaatgcaa taagcattgt agaacctaga ctatcagcta
    44761 catttgatag tggtgctcct aaataagtat ttaaaatatc tgttaagaaa cttccattag
    44821 aaagcatttt tataaatatt cctattacag taaatatcat tatagttaac attcctggta
    44881 ttaactttgc aaatgatctt gaaactgctg gtggaactcc atctggcatt tttatagtta
    44941 atttctttac cttagataat cttacaaata tctcagttga taataatcct attaatatag
    45001 aagagaataa agctgctgta cctaagtagg tttgttgtat cattccccat ccttcaacta
    45061 ctgtagttcc tccctcagga actattttac caatttgtgg agccattata aaaaagatag
    45121 ataaagctat aagcccagat tgtaacccat cactctcata agattttgct agaaaataag
    45181 caacacctat tactaagaat actgccattg tacctattgc tccccaccaa aggtcgcctc
    45241 caaatgtggt ccagttttct ccaaatatac ttgccatcaa atttttgtat gcttccaatg
    45301 gtaagttatt tatcaatgtt cccaatgcac ctaccatagt tataggtatc attactataa
    45361 atgcatctct tactgctact aagtgccttt gagctcctat tttagcagct acaggcacta
    45421 tatatttatc cataaatgac atgaattttt ccattttaca tcctccagta caactttatt
    45481 ttgaactatt taactctaca gctctatcta acacagcttt tccattacat cttccataat
    45541 ccatcatatt tataacttct actggaatat tatatggttt tgccatttca gttgcttttc
    45601 ctaatacata tctaacttga ggccctaaaa gtaagacatc acaattttct atttcatttt
    45661 ttaagttcac ttctgatata gcccatattt ttgcttctat accgttttcc tttgctgcat
    45721 cctccatttt tgtaactaat aaactagttg acattcctgc tgaacaagct aacattattt
    45781 ttatcatttt ttcccctcca aattagattc attaaatatt ttttaagtca taagtttttt
    45841 aaactataca tttaaaatat gtttttctat taattttttc ttaatgtata tatcttattt
    45901 ttaaatgtat atacatttaa ttcttgatat tattatatac caaatgtata tacatttcaa
    45961 gaaaactttt tcattttatt taaattttat tcgacaacag cattcaaagt taatgtatat
    46021 ccatagtgat atgtgtgtga gtactcaaaa atctcactat tttgtaactt acaaatttgg
    46081 cttatatata aaagtggctc tggttcttct aattttagat tatccataca ctcttcattc
    46141 gatattacag cttgtacttc tctctctgac atggctatct tgtatccaca cacttcttca
    46201 acaaaattat aaagagaatt ttcacaatga gtcttattta aatttggaaa taacttaact
    46261 ggcatataag taatcatata tgaaactctt acatcattag ctaatcttac tctttttatt
    46321 tcccaaacat tttcttctaa acttatattc aacttacttg ataaattttc atctgcctga
    46381 actatttcaa gagataccaa tatattagat actttatagc ctttactttc catctcttta
    46441 gtaaatccac ttacagacga tatattatca tatatattat ttgcaataac tatactacct
    46501 acacctcttt gtctagttat atagccttct ttaactagat tattaagtgc agaccttaca
    46561 gtcattctag ttacattaaa ttcttcactc agctgttttt cagaagggat taaatcccct
    46621 tctttcaaac tgtctgaatt tattaactct ctaacatgat tttctataac tttataaatt
    46681 ggctctttca tttaattcac tccttattaa atcttttata taataagata attttatata
    46741 caaacaaaga aaatgtctat acatttaaga taatttaaat tttattcaga aatcttgtac
    46801 aactttccga gttttaataa agatataaat agacttatca ttaatatacc ttttaatata
    46861 cttgataagg ttatactcca ccatacacca tttagaccca acatttctgg tctagatata
    46921 aagtaagcca atggtactct tgcaccagtc agtatagtaa ttataataga aggtatatag
    46981 gttctcccta atcctttgaa agctcctgtt gttattattt ctaaacacat aaaaaattga
    47041 gaatagccta atattttaag ataaactgct cctttttcta ttgcctcact ttcatctata
    47101 aaaatagaaa atatactctc tcctgcaaac actaatacta aagtagttat tataccaagt
    47161 acagaagata cagttatagc tattttgcaa cctttattta ttctagaata cttcttggca
    47221 ccataatttt gtccaacaaa actgcctaat gcaactgata gcccttcagc cgtcatccag
    47281 gaaattgctt ctatttgtga gcctactttt tgtactgcaa cagccacagg cccccatgat
    47341 gctactatta ctcctaatag cattgagaat actgtaaaca taccactttg tattgctata
    47401 ggtaatccca atttatacag aactttataa tatttagtct ctatttcttt aaatagtctt
    47461 aatttaaaat attcttcttt acttttaact attatatata taaaacatgt agtaactact
    47521 atctgagcaa atacagttgc aatagcagct cctgcaacac caagttttgg agcaaatccc
    47581 catccaaaaa ttaatatagg gtcaagtata atattagtta ttaatcctac tgtattaata
    47641 cgaaaaggag ttttactatt tccaagacca ttgaaaattg cagtaaatac tggattaata
    47701 aaataaaata ccatacctag agctactatt atcaaatact gtctagacat ggttataact
    47761 tctaagtctc ctaatctaaa aaaacctatc aattgtttat taagtactat taaagaaatt
    47821 gtatatatta ttgctagtat aatatttatt tctattgctg acttcatata tgatttagta
    47881 gtgcttatat tatttgcacc aatactttgt gctactttta tctctccacc tacttttgaa
    47941 atcataataa atgccattgc aagccatgga aaaaaaccag ctgttccaac tgcagctact
    48001 gctttacttc cggctttacc aacccatatc atatctatca tgttataccc catctgtata
    48061 aatgaagtcc ccattattgg aagtgatagc ttaagtaatt tttctgtaat tcttccttct
    48121 gttaaattaa gtctgtcttt cattttccac ctcttttata ttaaatttat ttgcttatat
    48181 agctagatta atcattttat ttgattgagt tcactatcct tcaatttata ttatcatatc
    48241 tcaggattgc atctcaatca tacttatgtt taagatacag tattttacct atctatgata
    48301 aaattaatac ttatgaatat aaatctagcc atttcatcaa tacatattct aacctttaac
    48361 ttaacttcaa ttaagtatac atatcgtaac caattagaat attttatacg gtatcttcca
    48421 ttaataaaca accaatatca atgatttata tataatttta tttatacata aaatagtata
    48481 ctataataat aaaaaaacta tatttgaatg ctttaagcat gtttactaat tgtaatttcc
    48541 acacaaattt gataatatat gaatatatta tggtgaaatt tatagatata tattaattat
    48601 ggggagattg tgggaggttc ttttcacaag acatttaagc tatgaaaata aataaaaaat
    48661 tcaatagagt tgatatgcag gtttcaatac ttactgcaat tattgtaata atatcttcct
    48721 catgtgtata tttttttaat tatacactaa cttataagag catgatatac tctttaagtg
    48781 agaggtcaaa acatatctat gaatatgtag aaagaaatat tgataaaaat acctttactg
    48841 aaattaattc taaagaagac caatcaaaaa agtcttataa aagttctaaa atccttttag
    48901 aatctgtaaa aaatactact ggagtcatgt atctatatac tgctaaaaaa acaaaagatg
    48961 gatcactagt atatgttgtt gatggtttaa attcatctag gagtgatttt agaaatgctg
    49021 gtgatttgat agaaaaagag atttggggag accttaacaa agcattagat aatcatatat
    49081 tgttacctaa aaagattaaa tcaacatctt ggggatacat atttatatcg tattttccta
    49141 ttcacaatga tgttggtaag gttgttggag taataggaat tgaatttgaa gcaaaacatc
    49201 aatacaatac atttaaattt ataagtatag ttacaccaat aatagcatta ttaacttgtc
    49261 taatatcagc attgattgca gtgattctat ttaaaagaat atctaatcct acatatactg
    49321 attttgctaa tacagattat ttaacaggcc taaaaaatag aaatgctttt gaaatagata
    49381 tgaataactt aaacaattca aatctaaaga atctaatatc tatcatatca atagatttag
    49441 acggtttaaa aaagattaat gataaatttg gccatcagac aggagactta tatattaagc
    49501 aagcaagtaa aattatacaa tatgtaataa ataaaaaata tatagtttat cgtattggag
    49561 gtgatgaata cataattatt ctaaaaaatt tatctcacaa agaaataaat aatattataa
    49621 ataatattaa tttaaaaata gttgaagaaa actcaactaa tgaattaaaa ttatcaatgt
    49681 ctattggata tgctattttt gatgaaaaag tagatgtttc tttatttgat acatatcgtc
    49741 gtgcagattc tcaaatgtat gataacaaaa ggaaaattaa taaacaatat caataataaa
    49801 tttaaaaagt ctattcttta aataataaat cacaagaata gactttttta aatttgtgtt
    49861 tttataagat aaattattta attaaagttt gttctttttt ctaaacgatt ctttataaat
    49921 tcatatatta ctttcactaa tatatttgtg actaatatta acacagctag tgatgctgcc
    49981 tgtgcagtac tccccttatc atctagttgt acaattgata ttgcagctac ttttgaactt
    50041 gcagtatata taaaaactaa tgctgatatt gtcaccatgg agtttaggaa aaagtacata
    50101 aagttctcta aaactgctgg caaacaaagt ggtatagtta cgttcttaag aattgatgcg
    50161 ctgtttattc ccattgattt agctaccata tccaattctt tatctacttt cttaatagca
    50221 gtagtagctg ttataaatgc tactgaaaag aaatgaacta tattacatat aaccattata
    50281 agtaatgttc catataatcc attaaacaaa ttcttaatgt atattgtatc cataaaatta
    50341 aactctaatt tattaaaaaa taatacataa gctataccta aaactaatcc tggcaatgcc
    50401 ataggcaatg tacttaaaaa atacccaaat gattttaaat atggagcttt ttctgttttt
    50461 tcattcaaat atgcaaataa gaaaacaaag ctcgtcccta gaatagctgt caatagtgac
    50521 atttttattg aatttataaa tggtttaatc ccatcaatac tgtttttcat agaaaaatgt
    50581 tccagtgtca gtcttaaatc atatggccaa actgatatta atgaagataa gattacagtt
    50641 aacattacag ctattatacc aaatgcaata attcctacaa cccctcctaa aatactatct
    50701 cttaacttat tagttttaac cttatatgga attgactttg aagttatata tgaattttga
    50761 tttttttcaa tttttttagt tactataaac gatattattg caggtattaa aagtaatatt
    50821 gatacagttg ctcccattgg catattctgt tgccctataa tttgcttata tacatctatt
    50881 gataatacat taaactctcc tcctatcacc tttggagctc caaaatctgt aaaacataat
    50941 gtaaacgata cgaatgcaga acttattaaa gagtatttta tacttggtat tgttatgctt
    51001 ataaattctc ttatcttgct tatccccata gtattactag cttcatatag tctataatcg
    51061 gttgaactta gagatatata aagtatttga aatgattgag gaaatacata tataatttgt
    51121 gccaatatta ttcccactgc tccatatatt ggaacatgca ctccaagtaa attagttacc
    51181 aaaccttgat ttccaaataa atatactaga cctattgcat gcatcattgt tggagcaaac
    51241 ataggaaaca tagctaatga tttaaaaaaa cctttaaatt taatctttgt tctagttaat
    51301 gcatatgcat atactaatgc aagtgttagt gatattacag ttgatataac tgatacaaat
    51361 actgtatttt ttagagaatt taatagagat tcagaagcca agtattcttg aaagttttgt
    51421 aatcctacaa agactccatc tttatctaaa aaagcctgta caaatagata tattataggt
    51481 gctaataaaa atattactaa agacataact aatactgtaa gcatacattt ttttatatct
    51541 aaattcattt ttgatttaag acttatactt ttagaactta tagcattatt attcatacac
    51601 ttatgctcct ttaacttttg gatactttaa atagtatttt tcgtctatac ttatttttac
    51661 aaggtcacct tctactaaat ttaaattaac ccactctttt atagaagcat cacttattat
    51721 gaaattttca tttaatttat cttttatttc aactctcaat aaattccctc taaactctat
    51781 atttgatatt attccttggt agttttcttt tgtcgatttc tctatttgaa catattctgg
    51841 tcttacagtt aatatagaat ctccattatc aaaacagttt gtatctccta taaattgagc
    51901 tgtaaatagg ttttgaggat tttggtatat ttcttctgga gtccctattt gcattatatc
    51961 tcctccattt agaatagcta ttttatctgc catagttata gcttcttctt ggtcatgtgt
    52021 taccattatt gtcgttatat taagttcttt ttgtaatctt ttaatgtcca ttctaagctt
    52081 gtgtctaacc tttgcatcta atgcggacat tggttcatct aaaagtaaaa actttggttc
    52141 aagagctaat gctcttgcta ttgcaatcct ctgttgttgc ccacctgata atgctttagg
    52201 atatttatga gcttcattag tcaatccaac tgtttcaagt acttccttaa ctttattatc
    52261 tatttttcct tttgaaactt ttctctcttt tagtggaaaa gctatattgt tataagcagt
    52321 catattcgga aataatgcat atgattgaaa tactatacca aaacctctct ttgatggctc
    52381 taaatttgta atatctttat cctggagaat tatagttccg ctatttacat cttcaagacc
    52441 tgctattatt ctaagtaatg tagttttacc acatccacta ggtccgagta aacatagaaa
    52501 ttctccttct tcaatatcca aactaatatt atttaaaacc cttttttggt cataactttt
    52561 aaacacatta tttattttta agtaactcat atcttatctc tccttatata gttatttttt
    52621 tattttaaat tttatatcct actttttctt ctaaaattct ttcatctaaa ttattaattt
    52681 aaactttact tactttcaga ttttgaaccg tattttgatt cccaagtttt aagtactgta
    52741 cttctgtttt tggcagcaga gtttaaatca acttctttta taagctgttc aaatggttgt
    52801 ttagaataac cttttggaac tggattatct gtctttatgg aagtaactgc tacttcatgt
    52861 gcatatctat ccatagctga atcacttatt gcccaatcta aaaatagctt tgcttctttt
    52921 tttatattat cttttttaac taaggcattt gcttctaagt cccagcctga accttccttt
    52981 ggaaatacaa cctctatagg ttctccacta gataattgtt gtgtagctct atatccaagt
    53041 gtaataccta ttggatactc tccagagcca gctaacttcg caggttttga acccgagtgt
    53101 gtatatgtgg ctatattttt atgtaattta tccatgtaat tgtaagcatc tttttcaccc
    53161 atcatttgaa taagccctga tactgtaaga taccccgttc cagatgatgc tggatttggc
    53221 attgttataa gtcctttata ttctggtttt attaaatctt cataactttt tggaatatct
    53281 atacctagtt tcttaagttc cttataatta actactattg ctgtctctgg gacactcatt
    53341 cctacccaag atacatcatc ttcagtatct ttaaattttt tatcaacttt atcataattt
    53401 ttaggcttat atgcttcaag tgcaccatct ttttttagct ctatcatgtt cattgctgat
    53461 aatccccata taatatctgc ttgtggatta tctttttctg ctaatagttt tgaagctatt
    53521 acaccatgag aatccataac tatctctaca tctatattgg gatactcttc tctaaaacct
    53581 tccatataat aagctatctg ctcttcctct agtgctgcat atattgtaag tttattattt
    53641 ccttttcctt gagtagtttg tggtgttgtt gatatgcaac ttgtcataaa tgtaatcact
    53701 acaatgcaca gacaagtaaa tataaattct ttcatatctt ccccctttgt gttttttcct
    53761 ttttttattt gttttttatt gtttttgctt atgtttatat aatacaccta atttgttaat
    53821 ataaagttaa gtgtaaatta acagttcgtt aatttttaga aatttttgtt aaatatatta
    53881 tttttagtta aatacagtct gtttttaaac aatatattaa tttttatagt atctacattt
    53941 taatttaaac ataaaaaata agtctaacct ctataaagta gaatgttaga cttattttta
    54001 taagtattta attatacaac caaatcattt tttttctttt cttcaagtgc ttttttaagc
    54061 accatatcct taaccttcat aagtctagta gtacttgctc tctcattttc ttcaagcttc
    54121 atagctatat acttaatagt ctcaatgtaa tttggtatca ttacattttc aagagcatta
    54181 acacgtctac gtgtcttttc tatctcttgt gcaagaagct gtgcactttt ttcactttct
    54241 gcaagtctta aaagtggttg catagcatct gagaaagctt ccatagcaga atctaattca
    54301 cctgatgtaa aagcaagacc ataaggataa atatcacttt gattgttttc agttttaaag
    54361 tcaaacacag gaacatcaac actcattata tttctcgtag aaacatctac tgaaactgat
    54421 tgctttggca tcataagtgc tgaacccaga tactcctgac tcataactgc tcttgcgatt
    54481 ataaagtttt tataagcagt atcaagagca ttttctgctt cttcacgcaa tcgcttattt
    54541 tctcttacaa tctcaagaaa ttgtttcata agttcatcaa gtttatcttt aagaagctta
    54601 tgacctcttg tagcagtttt aaggagtttc ttaagtctag tcatttccat acgtgttgga
    54661 tttatattta atctagccac agatatcact ccttaggcat atatttttca aaatattcat
    54721 cacggatacg tttaagttca ctttttggta acatactaag aagcttccat ccgatttcta
    54781 atgtctgctc aatagttcta tcagttctaa atccttgtga aacatattct ttctcaaatt
    54841 catctgcaaa cttagcatac attaaatcaa tctcagaaag tgcagattca ccaagtatag
    54901 ccataagttc ttttgcatcc ttacctcttg aataagctgc aaaaagctgg ttcattgtat
    54961 ctgcatggtc ttcacgagtc ttacctttac caataccttt atcttttaga cgagaaagtg
    55021 atggtaaaac atctatagga ggctgtatat cttttttata aagttcacgg ctaagtataa
    55081 tttgcccttc tgtaatgtat cctgtaaggt caggaattgg atgtgtctta tcatcctcag
    55141 gcattgtaag tattggaatc atagttatag acccttcatg ccctttcatt tttcctgctc
    55201 tttcatacat agtagcaagg tcagtataaa gatatcctgg atatccacga cgtcctggaa
    55261 cttctttttt agctgctgaa acttcacgca acgcctctgc atagtttgta atatccgtta
    55321 ttataacaag tacatgcata tcttgctcga aagcaaggta ttctgctgct gtaagtgcca
    55381 ttctaggtgt agatacacgc tctacagctg ggtcatttgc aaggtttaca aacactacag
    55441 aacggtcgat tgcaccagtt gctttaaaat cgcttataaa aaattcagca tcttcaaaag
    55501 ttataccaac tgcagcaaat acaacggcga acttagaatc agttccacgt acttttgcct
    55561 gtcttgcaat ctgaactgca agattggcat gtggcaaacc agaccctgag aatataggaa
    55621 gtttttgccc tctaaccaat gtattaagtc catctatagc agagactcca gtttgtataa
    55681 actcggccgg ataatcacgt gctgctggat ttattggagc tccatttata tcaagtcttt
    55741 tatctggtat aatttctgga cctccatcaa taggtctacc cattccacta aaaacacgac
    55801 ctaatatatc tggtgaaact ccaaagtcaa gactctttcc taaaaaacga actttacttt
    55861 cagcaaggtt tatacctgtt gagctttcga aaagctgaac aagtgcagta tctccattta
    55921 cctctagaac tttgcaacgg cgaatttctc catttgaaag ttcaatttct ccaagttcat
    55981 caaattttac accttctacg ccgtttatta acataagagg accggctacc tcttgtattg
    56041 acttatattc ctttatcata gttcttcagc ccccctcttt ttaataaggt ctgcaagctc
    56101 gttatctatt tcatcttcaa ttgaatcgta agctgcatct atatcgcctt cttctacata
    56161 cttagcacga ccaatctttt caagaactga aagcttgata atatcgttta tagtaacatt
    56221 ttgcttgata gcatcttgtg ctgacttata gaattttaat ataagtccca tcattctata
    56281 ttgtttatga agtgatgtat aagtatcaac ttcatggaaa gagttttgat gaaggaagtc
    56341 ttctcttata gaacgagtaa cctctagtat caatctatct ccatctgaaa gtgaatcaac
    56401 cccaaccaat tgaactattt cttgaagttc agattcaact tgtaaaagtt tcatagcttg
    56461 agctctttga gctgagaaat tatcatttac atttttatct gcccatacat caactttttc
    56521 ctgatagagt gaataactag tcaaccagtt aattgctggg aaatgacgtt tatatgctaa
    56581 agatgcatca agaccccaga atacttttac tatacgaaga gttgcttggc tgacaggttc
    56641 tgatgtatca ccaccaggag gagaaactgc accaattgct gtaagtgttc cttctctttc
    56701 atccatacct agacaattta cctttcctgc tctctcatag aattgagcaa gacgtgaacc
    56761 taagtatgca ggataacctt cttcaccagg catctcctct aaacgaccac tcatctctct
    56821 aagagcctca gcccatcttg atgtagagtc tgccataagt gcaacactat atcccatatc
    56881 tctaaaatat tcagctattg taataccagt gtatatagaa gcttcacgtg cagcaaccgg
    56941 catatctgat gtatttgcta taagcacagt tctttgcata agtgactcgc ctgttcttgg
    57001 gtcttttaat tcaggaaatt caagaagaac atctgtcatt tcatttccac gctcgccaca
    57061 gcctatatat acaactatat ctgcatctgc ccatttagca agctgatgct gtgtaacagt
    57121 ttttccactt ccaaaaggtc ccggtatagc tgctacccca ccttttgtga ttgggaaaaa
    57181 tgtatctata actctttgac ctgtaacaag aagtgaatct ggtgttttct tttctctata
    57241 tggtctttct tttctaactg gccatttttg catcatagtt acaggtatat cattaccttt
    57301 ttcatctgta ataacacaaa ctgtatcttc aacagtaaat tcaccagaat atatttcttt
    57361 tatagtaccc ttaactccat aaggaaccat aattttacat tctactactg ctgtttcttg
    57421 aacgccacca attatatctc ctgctacaac cttatcacca acagattttt taggtttaaa
    57481 ttcccactta gttgttctat caagtgaaga tacttcaaca cctttagtta tattagtacc
    57541 tgaaatatca tacatttttt caagtggacg ttgaatacca tcataaatag ttgaaataag
    57601 acctggtcca agttcaacac tcataggcat tccaagagaa actacttctt cccctggtcc
    57661 tagaccggaa gtttcctcat aaacctgtat agaagcttta tctccatgca tttctataat
    57721 ctcgcctata agatgtttat ctgaaactct tacgacgtca aacatattcg catctctcat
    57781 gccctccgct acaacaagcg gtcctgacac ctttacaatt gtgcctgttt tcatattctc
    57841 tcacctgcaa tcatatactt tatcaaaatt taaatattcc ctcctatatt ttattagcac
    57901 atatatttag caccaataaa atttgagatt gtcttttatt caactttcat ctattcttta
    57961 aataatatat ctgcacctat agcacgttta ctagattctc ttacttcatt catacctaat
    58021 cccatacttc ctccaatacc tggaataact ataatggcag gaagttggta atctttgtat
    58081 tttgctattg attcttttgc aagcaatgat gcctgttctg taatataaat tatcgcatat
    58141 tcatcttcaa ccaacttatg tatacttttt ttaatttcgt cactatcata ggctgggaag
    58201 atatctattc caagagctaa aaatcccatg atgctatctt tatctccaac tactcctacc
    58261 ttatacataa gcatccctta gcctttcttt tatagtatct gcgtctatat tattaagctt
    58321 actagtcaaa ataattctaa ttgtttttat ctctgattct ttagcatata aatatgcaat
    58381 tagtggttct aatgtaagag gcttaaattt tgctcctcta acatattcca taagatagtc
    58441 atcacaaagt ttttcaaaga cagtaaaacc actatccatg ccatttttac agacatcaga
    58501 gtaagaagta gatttaaaac atgatgctgg agtatctgag gaaaaagctt ctaagaattt
    58561 ttccttatca agtgagccac ctgaaacaaa cacattcata aacatatcaa aatctttctt
    58621 catatttttg actcttataa aactctttag atttgttaaa tcacaaactt tcataacata
    58681 atctattaca aatttgtttt ggctttcttt agcagtttct ttcatactgg taaaggttgc
    58741 tttatctaga acaatatcaa ccatctggcc attttgtgtc ttattataaa catcaaatgc
    58801 ctctaatata gctgatgaca ttatcttagg taaatcacta aaactacgat ttgctaaaga
    58861 ttctctaagc ttaataagag gaattgtacc tccatcaatt aaatatttct caccatctac
    58921 accagaaatt tcttctttaa tcagcacttt taaattgtga tagtcatttt tataaagaaa
    58981 tacatcaaca aatttttctt caggagctag acttttaaga atttcatatg tctttgataa
    59041 ttcttgagtc aaaatattct caaaattatg tatattatta tttgatgtat cagcataacc
    59101 tgcctcagct ataattctta aagagtcttc agggcttttg gcttctgcca tttgaataaa
    59161 catttgtctg ttaagaagct gtttttcaag cactcttatt cgtgcaacgg catagggata
    59221 agttttttct tctgccattt tttaacccct ccttaattaa acaaaatttc tgctgcaatt
    59281 tgttcaatat attcatgctc tactgcaatt atggcttcaa atgaataatt gtactcaatg
    59341 tcaccttttt taactatgaa acctccagta atatctctag tttcatcaga cactttaatg
    59401 cctttttttg atagcacatc ttttaatgtt tttttatctt ttttagataa tactatctct
    59461 gaatcatctg tacaatttga tttttcaatc atgtttagta taatctcttc atattcatta
    59521 tctttaagat ttaaaagttt gtttttagct tctaaaatta cctcttctaa aatctcttgt
    59581 ttttttgata atatttgctt tttagcctcc atataagctc ctgatatttc cttagaagcc
    59641 gctttttctg cttctgcctc tgcaagcttt gtataggaag ccatttcctt ttcagctttt
    59701 tcattagctg aatttacttt taaatctgct tcacttttag ctttatccaa tatttcttgt
    59761 gcttcttgtt tggcatcagc tataattctg tcaatcattt tttgttcatt acccatagga
    59821 gtgcttcctc cttttcattt tttttactta tgaattataa tttataatta accaattgca
    59881 attccattgt aagctaagaa agaaacaagt agtcctaaaa gagcatatgt ttcaactatt
    59941 gctgcaaatg ttatagcttt accaagttct tcaggtcttt ttgcaactat tccaactcct
    60001 gctgctgctg ctttaccttg tgctatacct gatactaaac caactaaacc aattggaagt
    60061 cctgccataa gaagttgtaa accttgagct gatgttggta ttgcatctcc accaataatt
    60121 cctgcttttg ataaaataag gaaagctatg ataaatccat acagaccttg tgtaccagga
    60181 agaagttgta aaactagaag ttgaccgaat ttactagggt cttctgttac aacacctgct
    60241 gcggcttgac ctgctattga agtaccttta gcagaaccca tacctgcaaa taatgctgct
    60301 atagcagctc ctgatattgc caaaaattga ccatttccta acgcgtttaa aaattccata
    60361 tttacatctc ctttttatta atgcttgtat attttgtttt atacttaaac ggaacaaaag
    60421 gaactcctcc gccttcataa aacttattga aaaattctac atactgtaac ctacttgtat
    60481 gaacatatgc acctagtgca tttataagaa ggtttattgt gtgtcctaca acaccaatta
    60541 caactgcaag tatcggacca gctatagctc ctaaaaggtt gataacttgt gctataactc
    60601 ctgttgttaa acaaagcgcc ataattcttg tataagataa tatatcagca aaataacttg
    60661 ttataccata gagacttgaa aatcctttaa aaagcttaac aggaatacct ttaaagcttc
    60721 ttccttgagt aagtacaaga cctatagcac ctataattgc taaatatttt cctacttctg
    60781 aaaatacacc tatatcccct gcaacaaaag gtattataag tagacataca cctgttatac
    60841 acaaatacca aaaacctatg tcaaagattg catctattac ctttccactt cttattaatg
    60901 tatatgcttt tattcccaaa cctacatata tatggataat accgaacagt aacgacattc
    60961 ccataagtgt cataacatcc tctaaaggat taattaatgc ttttatagga attaaatccc
    61021 ccaaaatacc cccgaatata agaccccata cagtagttga aactccacag ataccaataa
    61081 gtttaattaa atttccttca cctttttgta tctttaattt atatacaaca aatccacaga
    61141 ctactgcaat tattaaccca tatgcggcgt cacttagcat cattccaaag aatacaacaa
    61201 agaagaaagt taaaattgta tttgggtcaa tatcttttgt acttggatag ctgtacatgt
    61261 tagttacact ttcaaaaggt gttactaatt tattgttttc aagcagaatt gggaagcctt
    61321 cttctttact tccttcttca gtctgaacac aacaattaaa cttttctgta atttctttaa
    61381 ttaattcatc tgcccttttg cttggtaacc aaccattaag acagaatgtt gtttttgttt
    61441 tgacaagtct ctctataatc tttttttggt ctctttctat agtgtaaaaa tcatataggt
    61501 tttcaagcat gtgtatacta tctgcatgtc ttttaatttc ctctgttttg tccttagaaa
    61561 cgttttcctg tctaattatc atttcattgt atttcttgat agcttgtaac ggtgtacctt
    61621 cttcatttgg taatgaaacc actgaaaaat taaattcttt taaaacttct aaaacatcat
    61681 caaaagtttc ttttaaagta attaaataaa catagttcat taacttgtca gaattgacaa
    61741 tcccaataac actttctggt atttcatctt ctaattttac tcttaaagca tcaatgtttg
    61801 cgcttatggg cacagttcca agtatagttt ttgtatactt agtttccata ttttcaagag
    61861 gaatatcaaa gttcacccat ggttctaaca tacttttatt gttgttaagt aagtttatgt
    61921 tagaccttat tgtacctatc tccttgtgca aattgtttaa ctccagagct tcagcataaa
    61981 cttgcccttc tttttcacta tcaattttgt taaactctct ttttggtgca agtagggatt
    62041 ttttttgttt cactagtgaa gaaacaatat taattgcttg gtttacatga gacattttct
    62101 gctctagcat tataacttca gattcttcac tatcttttac aagataattt tttaattcct
    62161 cctcctcagt aagaaaggca ctatcatcaa tctgaacaaa cccacggttc ataagtagct
    62221 ttaaaatctg gcttttttga gcttctaagc caacaataga tattttgttc atattaacta
    62281 ttgccatatt atctcactac ctttccaata accgcttcaa tagccttact ttttctttct
    62341 tcagcagttt tttcaatttt cagacattca cttttagcat tagctaagat tttatcaacc
    62401 tctgctctac tgtcggtgac tgccttctca ataagagttt cttccataag ctttgcttgt
    62461 ttttttacat cagcaaatat gctttcacat ttgtttacag catcagctct gatagactct
    62521 gcatcagtaa cagcttttac tttgatactg tctgctgtct gctctgcctc aacaatgctt
    62581 tttattattt caaaagccat ccaaatcacc tctcttacct ttttactatg tatataacca
    62641 attagatttt tttattctaa ttagattact acctcattgt taaagtataa acagcatttt
    62701 tatataataa ttaatatttt tcaacgttta tttcttaata aaacatattt tagaatagta
    62761 cttgcctaga tttacatcct tttttgactt acttttagac tatgacatga gtatggtttt
    62821 taaataccat tattttcaat aattataatt taaagcgata tagtttgtca atagcgttat
    62881 tttgttttaa aattaacgtt atgtcgacat tttaaatttt tattttattt tttttataat
    62941 tttaatttaa tttattacat ataaaagttt tttatattct ttttcaacct ctaaccattg
    63001 aagtttattg ttttttttga atttttagaa atatctcgca ctattttatc taacttttat
    63061 aaatcatcaa gctatatcga tttttgtcac cttattttta atctaatttt tttatatcta
    63121 gttagaaata aatcaatatt tttaaagatt gataaaactt acacctagtt ctaatagttt
    63181 tattttaagg caatgtatta atcttaagta gataatatca ttagttactc tatatattcg
    63241 acaacattat aaaaaaatcc tcgtaccaat cactccaatc ttcaatagga tatgctgctc
    63301 ctccatttac acttttcagt agattggatg tatattcttc tcccatagaa aaaagttccc
    63361 ctactgtcat atcataacct ctagatgtaa caaccttaca aaatgaatcc ataacatcac
    63421 gttgttctct tgtttttata agttcttttc taaattcatc atcattctta gcttcttcta
    63481 aaattttagt taatttttca ttcattatgt atttctcctt ttaagtagtc tttctgtaaa
    63541 cttcaataag ctttatataa aaaggaagcc atcctaaata aactaagatg acttcgctta
    63601 actgttagca acacatttac tagtataaaa taattggtgt acctggttct atattttcat
    63661 atattttctt tgccttagaa aaaggtgtat ttacacatcc atgtgaacct gaagacttat
    63721 atatacttcc accaaaagaa tttctccaag tagcatcatg aatgcctatg ttcccattaa
    63781 acggaagcca ataacttaca ggtgaactgt aaccttgacc agttaatgaa acatttctag
    63841 ccttataatt aagaggatat atacctgatg gtgtagcaaa tcctctagct aaatcacctg
    63901 ttacaacgtc tgttgataca agcatttctc catttttaaa gaaccacata tgttggccag
    63961 aaagactaat ctctacatat gtatttccaa tatcatcttt attacgagat acagcttttt
    64021 gtatgtaaat aggttctctt gtaacatttt taccagcttt tacaacatca actaatgctt
    64081 cagcttcttt ctttttatct atcaaccaac cataaattcc acctgaaatt gtaatatctt
    64141 tacctgtggc agttttaaat ggtcttgagt caccaaaagt actgtatttt ttacttaaac
    64201 ctcttgtata ttctaccata gcatcattat caaaagttat gtcataattc ttttctgagt
    64261 ctacttttaa ccatttatta atttcagaac catctaaagt ttccttacgg tcactaaaat
    64321 catatgtaac gactacacca acatacttat ctaatgtctt aatagcttgt ttcaactttt
    64381 ctgaatcctt tgtgttgttt ggtgcttcat aaactttact ttcatccaga gaaatagaag
    64441 tattcccaga attaattgag ttttgtacta acttataaaa atcatctttt ttcactttgt
    64501 ttccaagtac ctctggtact ataacataag cgtttttagt gctatcatac ttaggataag
    64561 cactcttagg ttctacaaca tttttactat caaaacaaga taaagcatta aacctttttt
    64621 ctagtaaatc tttattatat gaaagagtta tcatattatt atactcttta gacttaaata
    64681 ttccaccaat ccatccaata gcgctctgac tatcttttat cttttgaact ttatcatctg
    64741 gtgtaaattt taaatcaata tcttttgctt ttatttgttc tttcttattg tttctttcct
    64801 ctaagtttaa agtataggaa ttagtactag atgttaacaa atcatttact tgctcaactg
    64861 tttttcctga agcctttacg ccattaatag tcgttccaaa gtaaaaatga tttctaaaaa
    64921 agattgccat tcctatataa gctacaaaca aaacacctac tactatagct atcttaaatt
    64981 ttagattttt ttgtccactt tcttgtttta cctcttttcc atcaatcata attaaatcct
    65041 tccttacatt gtaattacct atgtacctta ttggttttta aacaaaacca tgtatgcctt
    65101 attttatata cccaaattac tgtatcatta tttatatttt taaatatata gttatttcta
    65161 taaaaccatt ttatctttta gataaaatga ttattataaa tccccccttt caaaatccct
    65221 taaacacaat ctctgctata ttaatatatt accatacttt taataatcgt gggactatct
    65281 atctattttt gtaacactaa actactattt taaaaaagaa actaattttg tgttagtttc
    65341 tttatcttaa cttattttta tattgaattt tagcttaaca taacgttgtt atatagattc
    65401 tatttaatta ttttagtttc tatatctttt ccatctgcat attctttaag taattcctta
    65461 gcatatccaa aatattcttc aatatcagat tcttgactaa aagaaaatat acaaattaat
    65521 atttcatttg catcttctgt tatcattgct ctttcagaat cactagttgg actcccaaat
    65581 ttacccactg aatcagataa tataggaaga ttttctatat ttattaattc tttcccaata
    65641 cccttatact gttctccttc atctcctaca gtaaaacata ctggtgaatg taatttgctt
    65701 acattatatg tacctactgg atataatgat tttattgata ttaaattgtt tatttctact
    65761 atattattta ttgtataaag acccttccct tgaataattc ttctaaccaa tgcttctgaa
    65821 gatgttctat atctacttgg attttttcca agctctttat aagcatttct tgcatcttta
    65881 attttatcta gtttcgtaat gtcttcaact tttagacttt gatttatagt atcacaaaac
    65941 tcatttattt cattaattaa tttttctgag cttttattta ctgctacttt tgcttgaatg
    66001 caacccagct tagcattagg ccatctttcc ttaagtttgt catcaatact tatatctatc
    66061 atacttaaac aactcctata cactaaattt ttattaaagt ttttatcatc tatacattat
    66121 agtactattc ttttgttaat aacatgttta ttagcaaaat aaatttattt ttaatataat
    66181 ttttataaaa tacaattatt ttttgcttta tattagacaa tacattgata aaataaaaaa
    66241 acactaatta tttagcaatg atttattaaa atcaagcatt cataatagtg ctttatataa
    66301 aaatcaaatt ttgttttaaa gtaaatctaa aaaattctta gctggcattg gtcgtgcaaa
    66361 ataaaatcct tgaactaaat cacatccaaa ttgtttcatt aactcagctt gttctttact
    66421 ctcaacacct tctataatta caactaaatc tagtgatttt gctagagaca ctattgattt
    66481 tgctatattg cgacttctct catcatgttc aatgccacca agaaaagact tatctagctt
    66541 caatatatca attggcatat ctcttaaaca actaatagaa gaatatcctg taccaaagtc
    66601 atccatagaa atagaaaatc catacgtcct caactcattt aaaacattct ttaaaatatt
    66661 taactcatta atagccacag tttctgttat ctctatttca atgaaatctg aacttaaatt
    66721 atacattttt attgttttat tgataaattt tacaatatct ggttgataca actcgaccct
    66781 agataaattt actgaaactg gaactatctt tttaccttgc tttttccatt catataaatc
    66841 attgcaaacc ctttcaaata caaatctccc aatttttaaa atcaacttac ttttctctgc
    66901 tattggaata aatactgcgg gacttataaa accatgttct ggatgattcc atcttattag
    66961 tgcttcactc cctatcattt ctccagttac aagtgaaaac ttaggttgat aatacacttc
    67021 aaattgattt tttaccaatg ctatttttat atcatctaaa atcattgatt cctcactaag
    67081 cttattccta acatcttcat tataaatagc ataatttata tttttacctt tagcaacaga
    67141 cctagccata tttgctttat ttacagcctt ttgaatatct acttcacctt cttcaacaaa
    67201 ataaataccc acacacagag aaatttcaaa gactatactc aaattacttt caatattact
    67261 tcgtatatta tcaagttttc ttataatttt atttcttcca tttttataat cacagaaaat
    67321 tgcaaaataa tcacttgaaa gtcgtgcata tacctcacct tctgttaatt cttcttttaa
    67381 agcttttgct attgtaatta ataaattgtc accaaattca tatccaaaag tatcattaat
    67441 catcttaaag ttattaatat caaaatatac tataatataa tttaaagatg gtttatctaa
    67501 aagatactta ctacattctc tataaaattt attttgattt cctatatatg taactttatc
    67561 ttcataagcc aaacttaata tttctttttc attagatttt tttatataaa ttatataaaa
    67621 aataattgca ataaatatta atacaataga taacactgca tatacagctc tgttgattat
    67681 ttgttttgaa ttactaaata caacagtttc tggaattgaa aaaattaaat tccagtcttt
    67741 tatataatta ttttcactat tcccattatc catagtggca tatcctaaat atcttctttc
    67801 ttccaacata tttaatacag ttgcacctgt tttattttct tgcaattctt tttttaatct
    67861 atttacttct tgaatatcat tatcctgttt taacaattta taaatatttt ttgataggct
    67921 atccccttgt ggatgaagga taattgtacc attatttttt acaacataag atatactatt
    67981 attttcataa aaagatatat taataagttt catcagagta ttgatattgt aagtacaata
    68041 taaaactcca actactttat cattatttaa taatggtact gaaatagcaa tcttctttat
    68101 agacttattt acttcatcaa catatggact tgaaacatac ttcttacctt taattgagtt
    68161 tttgaaaaat tctctatctt tcactgaaaa cttatcacct ttggtatttt cagcattacc
    68221 atttatatca actattgcca tagtgtaaaa tttttgattt tccatttcat ctaaaagatt
    68281 tttcattttt tcctcattag gtatatctgg atttgaagca taatagttag aaaaaatatt
    68341 taaaacatct aaatcttttt caatttgtct ttgtgctagt tgagagcctt gtactgcaac
    68401 ttcttttata tgtatttttg cctctgacat aagaagatgt tttgtatctt ctatatagat
    68461 ataccccatt gtcaaaaata atgatgttaa aaaaagaatt gtaattaatg taaaactttt
    68521 atttctccct gtcattcgac tcctccccta attagtcaat acttcatata gataatatat
    68581 cctttttata ccaattcatc atttatattg tatataacta ttcttaaatg ttacacataa
    68641 aatcaacata ataaccataa aaacattctt ttattattat aataacacta tttaattgaa
    68701 ttttaaatta aagaataaaa actatccatt ttctcaaaca aaaaaactct tatacaataa
    68761 aactttgttt tatgtataag agtttttatt ttatctattt tatagcaaac ttatcttcca
    68821 taatatgaat ttaaatacat atcttttatt tcattcataa gtggatatct tggatttgct
    68881 cctgtacatt ggtcatcaaa tgcttgaatt accatttcat ctattgaatc cataaactta
    68941 gattcttcaa cgccaaactc ttttatagat ttaggaatac caactttaag ttttaaatct
    69001 tcaatagcta ccaataaact ttggaatttc tcttcatcag aatttccttt taatcctaaa
    69061 aatgaagcta tttcagcata tctctttaat gcttctggat atttatattg agagaatgct
    69121 cccattttct ttggtgcatc tgaagaatta aatttcataa cttctgttat caaaagagca
    69181 tttgcaacac catgtggtac atggtggaaa gcacctaatt tatgagccat agagtgacaa
    69241 acacccaaaa atgcatttgc aaaagccata cctgccattg tagaagcatt tgccattttt
    69301 tctctagctt ctgggtcttt tgcaccatta tcatatgcac gtggaagata ttcaaatata
    69361 ctcttaatag cttgtaaagc taatccattt gtataatcgg ttgcaagcat agatacatat
    69421 gcttctaatg catgagttaa agcatctata ccagaagcag atgttaagct ttttggcata
    69481 ttcatcatca tatcagaatc aactatagcc atatttggca tcagttcata atctgctaat
    69541 ggatatttta ctcctgtatc ttggtcagtt ataacagcaa atggtgttac ttctgaacca
    69601 gtacctgctg atgttggtat agctacaaaa ttagcttttt cacccatttt agggaatgta
    69661 tatacccttt ttcttatatc cataaatctc atagctaggt cttggaaatc tacatctgga
    69721 tgctcataca atgtccacat tatctttcct gcatccattg cacttcctcc acctatagat
    69781 attattacat ctggattaaa ttctctcata gctttagcgc ctatcttagc acattccaaa
    69841 gttgggtctg gttctacttc aaagaatgta gtgtacttaa tattcatttc atctaataaa
    69901 tcagtaactg ctttagtata tccattattg taaaggaatg tatctgttac aataaaagct
    69961 ctcttcttat tcattacatc tttaagttct tttaatgcta ctcctaaaca accttttttg
    70021 aaataaactt tttctggtgc tctaaaccaa agcatatttt ctctcctctc agctactgtt
    70081 ttaatattaa gtaaatgctt aactcctaca ttttcagata ctgaatttcc accccaagac
    70141 ccacatccta aagtaagaga aggagctagt ttaaaattat ataagtcccc tataccacct
    70201 tgagaagaag gagtattaat taaaattcta caagttttca tggctgaagt aaacttatca
    70261 agtttttctc tttgatttac atcatcaata tataatgaag atgtatgccc aaatccacca
    70321 tcttctacta atttttctgc ttttctaact gcatcatcaa aatcacttgc cttgtacatt
    70381 gcaagtactg gtgaaagttt ctcatgagca aaagcttctt ctatttcaac tgattctact
    70441 tcacctatta aaaccttagt atctactggg acttcaaagc ctgcaagttt tgctatagta
    70501 caagctgttt gcccaactat tttagaattt aatcctccat ttactaaaat tatatttctt
    70561 attttttctg tttcatcttt atctaataaa taagcaccac gctctttaaa ttcttttttg
    70621 acttcattgt atatattttc taatactata accgattgtt cagaagcaca tatcatacca
    70681 ttgtcaaaag tcttagatac aagaattgag tttacagcag tttttatatc tgcactatca
    70741 tcaattattg ctggagtatt acctgcacca actccaatag ctggttttcc agatgaatat
    70801 gctgatttaa ccattccagg acctcctgta gcaagggtca aatccacttc tttcataaca
    70861 gttgtagtta attctaaaga tggctcatct atccatccta ttataccttc aggagctcca
    70921 gctaaaacag cagcctcaag aacaactttt gcagcctcta tagtagaatt ttttgctctt
    70981 ggatgtggag agaaaattat tccatttcta gtttttaaag ctaaaagtgc tttaaagata
    71041 gcagtagaag ttggattagt tgtaggtaca actgctgcaa taacacctat tggttcagca
    71101 attttagtca taccaaatgc ttcgtctttc tctataacac cacaagtttt agtttcttta
    71161 tatgcattat atatatactc tgaggcataa tgattcttta taaccttatc ttcagcaatt
    71221 cccataccag tttctttttg tgccattata gctagaggaa ctctttgctt attagctgcc
    71281 aaagatgctg ctaaaaaaat tttatctacc tgctcttgac tatatgtagc aaatattttt
    71341 tgagcttctc tgatttttgc tagtttttta actaagcttt caacgttatt tacagtatct
    71401 aaaccaactg acataacttc attttctttt ttattttcta ttattacttg ttctttatga
    71461 gccattttaa caacctcctg agatagtttt ttatttatta tgttaattta ttaacattat
    71521 agcatttact tttcctattt tcaagttatt tttttaacat tttatttttt atctaatata
    71581 acatcatttc tactaataat atctagcttt acaaatactt aatttaacta gatatcactt
    71641 tattttttac gcaaatcact aatgaaatca tctaaaaact taaggataac gttatttttt
    71701 taacgtttat atttttgtat tttttgcaaa taaaacttta taaaaagaat ttttaatatg
    71761 atactctatc aagtttaaaa ataaaaatcc ttgaaaatga aaattaaaac ttaattaaca
    71821 agttaaagta aatatagtta aatgtacaaa cat
//
LOCUS       DAMJVK010000007        64057 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.007, whole genome shotgun sequence.
ACCESSION   DAMJVK010000007 DAMJVK010000000
VERSION     DAMJVK010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 64057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64057
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.007"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            86..1282
                     /locus_tag="PC414_000473"
     CDS             86..1282
                     /locus_tag="PC414_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDF2462886.1"
                     /translation="MKILVLNCGSSSLKYQLIDMNNEEVLCIGLVERIGIEGSILKHE
                     KAGRDDKYVVEQPMKDHKDAIALVLEAVAHPEFGAVKEMKEIDAVGHRVVHAGEKFAT
                     SVVITPEVEEALKECIDLAPLHNPANIMGIDACKAILPDVPMVGVFDTAFHQTMPKSS
                     YLYGLPHELYTKYGVRRYGFHGTSHNYVSQRAAEILGKDIKDLKIVTCHLGNGASIAA
                     VDGGKCVDTSMGFTPLEGLIMGTRCGDIDPAILPFLMRKEGLDADGLDKLMNKESGVY
                     GMTGISSDFRDIEDAAKNGDERAQATLEAYVKKVQKYIGAYAAEMNGLDVVVFTAGVG
                     ENGKAIRADIASNMEFLGMKLDKEANDVRGKETVISTADSKVKMLLIPTNEELMIARD
                     TLRLVK"
     gene            1375..1899
                     /locus_tag="PC414_000474"
     CDS             1375..1899
                     /locus_tag="PC414_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HDF2462887.1"
                     /translation="MKVSIEKINRKETDKIDLNFCEKIDTISYCDEIYKLVSPVNLKG
                     KVSKTNKGLYLDIDVNFTIVDNCSRCLKEVEIPLEYSIQGFLVKEEDYDEDEFEEFDP
                     FIFDGEEIDLIDIIEQTLDFNVPHKVLCSENCKGLCQVCGANLNEEECSCSEITNDEE
                     YIDPRFAKLKDLFN"
     gene            1924..2100
                     /gene="rpmF"
                     /locus_tag="PC414_000475"
     CDS             1924..2100
                     /gene="rpmF"
                     /locus_tag="PC414_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDF2462888.1"
                     /translation="MAVPKRKTSKSNTKMRRAANSKMEATGFVSCPQCHEPKLPHRVC
                     PDCGYYKGKEVVSK"
     gene            2307..2864
                     /gene="fapR"
                     /locus_tag="PC414_000476"
     CDS             2307..2864
                     /gene="fapR"
                     /locus_tag="PC414_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HDF2462889.1"
                     /translation="MKKKSKAQRQKELIDMLKTDPFYTDEELSSLFDVSIQTIRLDRM
                     SLNIPELRERVKSIAETQSSKVKTLGVKEITGEIIDLSVGRLGISMLEVTQDMIYSKT
                     NTLKDTYIFSLADSLAMAIIDAPKVIMRVANVKSFKLIEQQDRLIAKAEVYRNIDKKH
                     YVKVVINNKAQEQIFRGKFIFEELD"
     gene            2875..3897
                     /gene="plsX"
                     /locus_tag="PC414_000477"
     CDS             2875..3897
                     /gene="plsX"
                     /locus_tag="PC414_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HDF2462890.1"
                     /translation="MKIVIDGMGGDNAPKSNVEGAVNAIKEYQVDLIITGDKDLLEKE
                     FSNYEFDRNKLEIVHTTEIIENEDKPVKAIRSKKDSSMVVALNLVKEGKADAIISAGN
                     TGALLAGGLFVVGRIKGIDRPCLCSAIPNVKRGMTLIADCGANADCKPKNLVEFAAMS
                     NIYSRKVLGLENPKVALANVGLEEGKGNDLVKRSYEEIKKLDLNFIGNVEAREVINAY
                     TDIIICDGFTGNILLKSAEGVALSVMSLIKETFMASTKSKIGALLIKDDLRKLKSFID
                     YSEYGGAPLLGLNGGVIKAHGSSDAKAIKNAINQGIKFSKGKVVEDINQFISKYNEEN
                     KNNEDE"
     gene            3923..4909
                     /locus_tag="PC414_000478"
     CDS             3923..4909
                     /locus_tag="PC414_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HDF2462891.1"
                     /translation="MNTKAGILGVGSYLPEQSYDNFHFEKIMDTSDEWISTRTGIKER
                     RFAKESEATSDLASKAALKAIECAKLNVEDIELIILATITPDMSLPSTACIVQDAIGA
                     VNATAFDISAACSGFVYGVTIAKQFVETGCYKNVLVIGAETCSKFLNYDDRTTAVLFG
                     DGAGAAVIGPVNEGGILSTHMGSDGKGKDCLKVPAGGSRLKASKETVEANLHTIEMAG
                     SDVFKFAVRKMAETSLRALEKANLNTTDIDYLVPHQANIRIIQASSKRLELDMKKVYV
                     NIDKYGNMSAASIPVALDEAYREGKIKKGDNVVLVGFGGGLTWGASVVKWTL"
     gene            5018..5947
                     /gene="fabK"
                     /locus_tag="PC414_000479"
     CDS             5018..5947
                     /gene="fabK"
                     /locus_tag="PC414_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HDF2462892.1"
                     /translation="MNKICKILNIKYPVIQGGMAWVATASLASAVSNAGGLGIIAAGN
                     APKEAIKKEIVECKKLTDKPFGVNVMLMSPFVDDIIDLIIEEKVQVITTGAGNPAKYM
                     DRLKEAGTKVIPVVPTIALAQRMEKLGATAVIAEGTEGGGHIGELTTMVLVPQVADAV
                     NIPVIAAGGIVDGRGIAASFALGASAVQVGTRFICSEECSVHSNYKNLVLKAKDRDAI
                     VTGRSTGHPVRTLKNKLSKEFLKMEQNGATPEELDKKGTGALRFATVDGDIEKGSFMA
                     GQSAAMVKEITPCKEIIEAMVNQAREIMPAIEL"
     gene            5963..6913
                     /gene="fabD"
                     /locus_tag="PC414_000480"
     CDS             5963..6913
                     /gene="fabD"
                     /locus_tag="PC414_000480"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDF2462893.1"
                     /translation="MGKVALVFPGQGAQYVGMAKDLYENNAVAKSVIDEASDALNMDL
                     KNLMFNGNEEELSKTENTQPAMVTHSVAVLKAVQAQIDLKYNACLGLSLGEYSALVAA
                     DAIDFKDAVCLVKKRGKFMQETVPAGVGAMAAVLGLDRNVLENVVKDIQGGIVEVANY
                     NSPGQIVISGENEKIEEAMVKCKEAGAKRAVKLNVSGPFHSSMLKEAGVKLASELEKV
                     NITKPKVDVVANVNADYYKNEGKDLLIKQVSSSVLWEDSIERLLNDGYDTFIEMGPGK
                     TLKAFIKKIASNKKANVNIYNIDGIDSLNEFVQNYRNGEI"
     gene            6913..7662
                     /gene="fabG"
                     /locus_tag="PC414_000481"
     CDS             6913..7662
                     /gene="fabG"
                     /locus_tag="PC414_000481"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HDF2462894.1"
                     /translation="MINLTGQVAVVTGGSRGIGKEIAKKLASFGADVVINYTSKEDEA
                     LKTKNEIESMGVKCTSIKCDVSKFDEVNQMIDSVVSEFGKIDILVNNAGITKDGLLMR
                     MKEEDFDRVIDINLKGVFNCTKAVTKPMMKKKYGRIINMTSVVGIMGNAGQTNYCASK
                     AGVIGFTKASARELASRNININAVAPGFIETDMTKVLSDDVKESTLANIPKKSYGKPE
                     DVANAVAFLVSDMSSYITGQVINVDGGMVMQ"
     gene            7716..7940
                     /gene="acpP"
                     /locus_tag="PC414_000482"
     CDS             7716..7940
                     /gene="acpP"
                     /locus_tag="PC414_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDF2462895.1"
                     /translation="MFNKVVEIIIEQLGVEDKEITMETSLMKDLEADSLDAVEIIMAL
                     EDEFGIEIPDTEAENFKSIGDIVKYIEANK"
     gene            7969..9207
                     /gene="fabF"
                     /locus_tag="PC414_000483"
     CDS             7969..9207
                     /gene="fabF"
                     /locus_tag="PC414_000483"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HDF2462896.1"
                     /translation="MNKRVVITGLGCVTPLGTGKEEFWSNIKSGVSGIDKITNFDAST
                     YQTQIAGEVKNFHPEEYISKKELKRLDKFAQFAIVSAKLAVEDANLDLDKVDRERFGV
                     IIGSGIGGVEAIETQHKILLEKGNKRVSSLFVPMMIGNMAAGQVSIFLGAKGPNTNVC
                     TACASGTHSIGDAFKVIQRGDADLMVAGGSEAAVTGLAFAGFCNMKAMSTRNDDPKTA
                     SRPFDKDRDGFVMGEGAGIVILEDLEHALARGAKIYAEVVGYGLTADAYHMTTPAENG
                     EGAARSMNMALKDGNVPLEEVDYINAHGTSTYYNDLYETMAIKTVFGEKAYDLCVSST
                     KSMTGHLLGASGAIEAVVCAMSIEDSFVPPTINIQEVGEDLDLDYVPNQGKEKNIRYA
                     LSNSLGFGGHNATIVLKKYV"
     gene            9433..11151
                     /locus_tag="PC414_000484"
     CDS             9433..11151
                     /locus_tag="PC414_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2462897.1"
                     /translation="MNFRLPKKRLPKKIIFICIILISIILFIKYDQNGLKEREIINKG
                     KYMSESLVADKEYDEAKKVVKTSFESIPEKKYNKVYNEVWDMLKTISYVPDGTKIVID
                     SLEKMRESDVKLSDECRFNIEKRLASVYIMDNNYSKAVDLTVKSIKLAEKLGNNYEKA
                     RLDVDLASIFLKIGGYETGEKLIKDSFKIDIDEGEKNGMVRLYAIINLAEIYVEEGEY
                     DKAIEISSRIKDYKKFVNTDNYNDFDIMASIMQSNAYVGKNNIQKAKTFLEKADYLIK
                     SDRTVYILDKDMYYYMAMGNLEYKSENYNSAIDNYKKSLEISTKRKLTQEKIKNLNKI
                     LNIYEKQGNIESLNEYQKMLINEYKENKSIRDSETSFYIIDKVSNESKLFEKTKKEIK
                     YYKILFTVILIAILGSTFLYNRLKYFKAQNLHDGLTNVYNRKSFDIMYEKYREKDDNF
                     ALVMIDIDNFKLINDNYGHKFGDTVIKGITNTICVMLEQDDKVFRYGGEEFSVLIRNK
                     SGDEVADIIDSIRVAIANKKWNDDVTVTISAGVAHVSDSKDVLEEADKNLYKAKQLGR
                     NKVVYK"
     gene            11182..13182
                     /locus_tag="PC414_000485"
     CDS             11182..13182
                     /locus_tag="PC414_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2462898.1"
                     /translation="MKQSIAIVTDKITKLSSFLEENIRLVLGNYIDINHYYLEVLKEN
                     DKIQGDFVLVMNDDRLNSMRKHLVQDSKIIVIRRTLKENEIYGLFSIPNGTEVLVVND
                     TKETTLETISLFYKIGVKNLKFTPYIEGKIYKNIDIAITPGVKGKVPSYINKVIDLGN
                     RYIDISTFIEIINGLRIDVKEIRKNLMAYSESLISLDNGVKDNYRQLFLKIEEQDVIL
                     NLSKDGIIFTSADGIINTFNKEALKILGINENPQGKNIEDIISEDLNILLEDKEIIDE
                     VVFTNKKYINVNKKSIFSMGNKAGIYYNLQEITYIKKLEQNLTNKLREQGQIARYTFK
                     DIKTKNKNMVKCVELAKKISKSDLSVLIIGESGTGKELLSQSIHNASNRSNQPFIAVN
                     CAAVPDSLLESQLFGYEKGSFTGALREGKKGLFELANNGTIFLDEIGDMPPLLQTKLL
                     RVLQEKQVMPIGSHNVINIDVRVIAATNKNLLKMIREGKFREDLYYRLNVLPIDVPSL
                     RNRKEDIITLMNFFLKNNIRLSPEVITILEKYNWPGNIRELQNVASYVSLMCDSMVFR
                     DDLPPYIRVECANNYEDGFGYTRKEFEEILRILYERRESSSGIGRGFIIEKLIQEGIE
                     ISESKVRKVLSYLSECGYISSKSGRGGSQITSEGIKLYNNIN"
     gene            13533..13778
                     /locus_tag="PC414_000486"
     CDS             13533..13778
                     /locus_tag="PC414_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HDF2462899.1"
                     /translation="MKYKTTGTCATEIEFEVKENKVTNVNFIGGCDGNLKGLKVLVEG
                     MNIEDVIQKLKGIECKTKPTSCPDQLSLALENYMNIK"
     gene            13813..14538
                     /locus_tag="PC414_000487"
     CDS             13813..14538
                     /locus_tag="PC414_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HDF2462900.1"
                     /translation="MKPIIGILGNLIIMENGMFPGLERSYVNNDYINAVLKGGGSPVI
                     IPVNTDKEVIKKQIEMVDGVLISGGWDINPQLYGEETREETTFIYPEVDEFDLIAISI
                     ALELKKPILGVCRGLQILNVSLGGTLYQDNNLIEGSYIKHTQSSKRHVATHKVDVKEG
                     SILEGILGKQLLTNSYHHQSVNQLGKGLKAIAYSKDGIIEAIEKEDENFVVGVQWHPE
                     MMVDYCDKMEKLFKYFINICSNL"
     gene            14560..15873
                     /locus_tag="PC414_000488"
     CDS             14560..15873
                     /locus_tag="PC414_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF2462901.1"
                     /translation="MNEKNKMGLISIILLGINAVVGAGVFLLPGDAMKSFGVASIFVY
                     IFDMLLVLSMAFCFAEVAGKFNKNGAAYVYTKEAFGDFCGFEVGLMKWVIGCISWGAL
                     IVGFPTSLSAVWAPAGEPHIQKIIIVAMIVGLTIINLLGVSLSKIVQNVITVGKLIPL
                     ILFIGIGIFFIKGVNFTSSTMVPPGAGATEFGAAALLMFYSFTGFESIAVAAEDMENP
                     QKNIPIAIISVIVIASIIYILNQVVCVGILGDSLSSTSTPVADAARICFGNMGAGLVT
                     FGTLVSVGGICMCGAFVNPRSCVALADDKMLPRIFARKDKKGTPYVAIIATMLITIPI
                     ALSGSFAELAAISAVARFIQYIPTSLSVLVFRKKRPELVGTFKTPFGAVIPLIAVCVG
                     TWLLFQASMHQLIMGLGALAIGVPLYFIMKSYNRKIYGENLKKIV"
     gene            16079..16618
                     /locus_tag="PC414_000489"
     CDS             16079..16618
                     /locus_tag="PC414_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2462902.1"
                     /translation="MPTITLKNGVDVLIREGVREDAQSIIDFYNEVGGETHFLSFGKD
                     EYKISLEEQENAIESAKASDNSVKLIAFIDGEIVGIATIDSNQKAKGKHVGVLGIVVK
                     EKYWGIGLGKRLMLDLIEWCKSNGITKKITLVTNEENYNAIGLYKKVGFEVESILKKE
                     CYYNGVYTDLIGMSLLLGI"
     gene            16707..18692
                     /locus_tag="PC414_000490"
     CDS             16707..18692
                     /locus_tag="PC414_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HDF2462903.1"
                     /translation="MKNLCKISDDYLNSKLKYLKLLSKQYPSISKASTEIINLEAILN
                     LPKGTEHFITDVHGEYEPFVHVLKNGSGVIKRKIEELFSNTIRDSEKKMLATLVYYPE
                     QKLDLIIKQEENIDDFYRINIYRLIELCKYASSKYTRSKVRKLLPENFKYIIEELLHE
                     HVKSEHKEEYYKSIVETIVDIGIAKEFIIAISTVIQKLVVDRLHVIGDIYDRGPRPDI
                     IVDKLIEHHCVDIQWGNHDILWMGAASGEKTCIANALRISARYANLDIVEDIYGINLL
                     PLATFAIEMYKDDPCKEFIPKVNDQSVTTTEKSLMAKMHKAISIIQFKLEGEVIRRRP
                     EFEMEHRLLLNMINYDEGTINLKGKTYKLKDTYLPTIDKKDPYKLTMEERNVIDKLVS
                     SFRGSEKLQKHVSFLFSKGSIYLKANSNLLIHGCVPLNEDGSFMSMNIMGKEYKGKAL
                     MDKMESLAREGFFFKDKAEEKLYGMDIMWYLWTGKCSSLFGKDDMTTFERYFIAEKET
                     HKENKNPYFKLRENEMACKRLFEEFDLELDESHIINGHVPVESKNGESPIKANGKILV
                     IDGGFSRAYQKTTGIAGYTLIYNSRTLQLVSHEPFNSAEEAIANESDILSTTVVVEHK
                     AKRKMVRDTDEGIKIQEEIEDLKLLLMAYKKGLIKEM"
     gene            18916..19884
                     /gene="spoIIIAA"
                     /locus_tag="PC414_000491"
     CDS             18916..19884
                     /gene="spoIIIAA"
                     /locus_tag="PC414_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HDF2462904.1"
                     /translation="MNKLSDEIINSLSTTIREKVEKVSNNNLNIEEIRLRSQKPLILN
                     ANSKDYFYNQKTMTLDLNQQNSYVVTREDVEQTFQIICKYSIHSFMDDIKKGFITLRG
                     GHRVGLVGKAIVEDGQVKNIKHISSLNIRVSREIIGCSDKILSHIIKGKNQINNTLII
                     SPPQCGKTTLIRDIVRNLSNGNEDYGFKGLKVALVDERNEIAGAYLGVPQMDVGIRTD
                     IIETCPKDLGITMLLRSMSPNVIVTDEIGSEKEIKALYTALNGGIGLITTVHGDSIED
                     IQNRKELNRLLDKELFKKVIILSAKRGAGTIEKIYDLEEKRWYFAN"
     gene            19874..20395
                     /locus_tag="PC414_000492"
     CDS             19874..20395
                     /locus_tag="PC414_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AB"
                     /protein_id="HDF2462905.1"
                     /translation="MQIKIIIIAFLIGSSYLIGEQIYKTYTRRHKQLNDLIRVLEILR
                     MDLSFGLYTLEEIFNRIGGNKEFCFWKFFYQISEGLHNEQSKTLEIIISENIDVLSKE
                     TYLGNKEIEELKNLILTLGKSDIESQQRMIDLSIENLKKQTYETKEDINKKGVLYKKL
                     VTFIGIGICIILI"
     gene            20412..20609
                     /gene="spoIIIAC"
                     /locus_tag="PC414_000493"
     CDS             20412..20609
                     /gene="spoIIIAC"
                     /locus_tag="PC414_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HDF2462906.1"
                     /translation="MEISLILKVAGVGILISVLNMILEKTDRKDWAGLTTLAGVIIVL
                     GMVITEISDLFNTVRTMFQLY"
     gene            20625..21011
                     /gene="spoIIIAD"
                     /locus_tag="PC414_000494"
     CDS             20625..21011
                     /gene="spoIIIAD"
                     /locus_tag="PC414_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AD"
                     /protein_id="HDF2462907.1"
                     /translation="MEIMQLIGIAIISTTLCLVIKKDRPEIANFIAIITGVIILLSVM
                     FKLNFIVDSIQDLANKANIPTMYISLIIKLIGIAYLMEFAIQLCKDCGEGNIASKLEF
                     GGKIIVMSMSFPILLSIVEMVVNIIP"
     gene            21096..22301
                     /gene="spoIIIAE"
                     /locus_tag="PC414_000495"
     CDS             21096..22301
                     /gene="spoIIIAE"
                     /locus_tag="PC414_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AE"
                     /protein_id="HDF2462908.1"
                     /translation="MKKRFLPMIIGFLFTFFFVNIFAIMIFANEVPSSEDKEYGETKN
                     SIDKYIDGQLDKLDINEIQDYINKEIVINDVNLKSFVKDLISGEKNILDLFNKDGLKI
                     LMFDEFKASLKVVAVILVLALLSSILKSLENSFSSGAVSQIATYIIFITMVSLTLIGF
                     KDVLQICYDAIDHTVGLMQVIMPILITFLLLIGFPITSTTLNPIFIGGVTFINVFFKN
                     FLFVSITVAFGILIINNLSKNIRLKRFFSFVKQINYVSIGAMFTVYLGLVSIQGLYVT
                     SFDKFSVKTAKFAIGNFIPVVGGFVSDSVDILLSSSQLIKNIFGGIGLILLVGICLLP
                     VIKILSVIVVYKLAAIIVEPVGEDGISNFLNEVANLMIIMLASVIAITVMFFVTVAIL
                     TSISVVSQG"
     gene            22361..22978
                     /locus_tag="PC414_000496"
     CDS             22361..22978
                     /locus_tag="PC414_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HDF2462909.1"
                     /translation="MLEGIKAWIVSVLIGAFIVNIVDMILPSSKIKPYVNLVLNFMFV
                     FIVITPVVGFFSKDMSLEDRILKSMGNYNKQYVDSTNALAKETGNNSLSKGYEDGLKE
                     VLKLKLDEYGYDLEDIELNGANINNIKIKEKNNSAKSNNSSNEENKNNSTKREEENIN
                     SNDKENSKQVFKKGTEYGLNLNEEKLKNDLIKVLDVSIEDIQIDK"
     gene            22990..23544
                     /locus_tag="PC414_000497"
     CDS             22990..23544
                     /locus_tag="PC414_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AG"
                     /protein_id="HDF2462910.1"
                     /translation="MLKNLNDKDKRKVYSLITIAGICVVSLVLLSCFPSSKSDKQVGK
                     TETNKNAEKQVTKEQEKDDLESKLTAILSKIDGAGDVDVMVTFESSEEIQPAFNSNNT
                     TETTEEKDAQGGERTVTTSSENKTMITSNSSDPVVIKTTEPKIKGVIVVASGASDPSV
                     KETLYSAVQTSLQVAGHQVEIYSK"
     gene            23571..24260
                     /locus_tag="PC414_000498"
     CDS             23571..24260
                     /locus_tag="PC414_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HDF2462911.1"
                     /translation="MKFNYKGRGFVIITLTAMLVVVGTVNYQLSKKSLLETSKEFKAY
                     EEAQLQKNTDESDSSKTEDSSNVDKQDGKESADIDIVDSKASKVKEKATETSKEIKAQ
                     LSSEKNMKKASYILDMKMNREKQRNELVQDLNEMINNPSTTEESRKEASNMKLNIVKI
                     QEKELQIENLLSTKGYEEALVYISDNKVNVVVNEAKLEKKDAAKIFDLVAEQANVKYE
                     NIKLTNNNSNK"
     gene            24370..24732
                     /locus_tag="PC414_000499"
     CDS             24370..24732
                     /locus_tag="PC414_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDF2462912.1"
                     /translation="MEDNKFGQVKISNDVIATIAGLAALEVEGIETTATLTDKLLKNN
                     GVKIQIEEEDVNLDVMVTIKYGMSIPDTAFKVQENVKNTVETMTGLKVSQVNIHIQGI
                     SFKKDKVDKEEAKATKKN"
     gene            24817..25323
                     /gene="nusB"
                     /locus_tag="PC414_000500"
     CDS             24817..25323
                     /gene="nusB"
                     /locus_tag="PC414_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HDF2462913.1"
                     /translation="MKKDRAQKSTTREYIMKLIYQININKEDFETLEDKVDNFLKDNS
                     EHIINRYKELALQYSKNTNLKLEDTEIEDVIDKKYINTVCKALKENHDKIDELINKHA
                     KNWTVDRMPKVDVSILRLSVCEILYLDTPNKVSINEAVELAKIYCDDKSPKFINGILG
                     SVVDEIGK"
     gene            25307..26377
                     /locus_tag="PC414_000501"
     CDS             25307..26377
                     /locus_tag="PC414_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-sialoglycoprotein endopeptidase"
                     /protein_id="HDF2462914.1"
                     /translation="MKLGNNIIIGIDTSCYTTSIAAISLDKKVIFNEKIMLEVRDNSK
                     GLRQSEAVFQHINNLGILSDRIKSFKDKFNVEGVCSSKKPRPVENSYMPVFNVGHNFG
                     KLLSSIYGCRFYETTHQENHIEASLLNSKLKNNNKFISVHMSGGTTEILLTSKQDSHH
                     NVCDTNLGKIAKISIKKDDKSKLYNNFGYNIDIIGGSKDISFGQLIDRVGIKLGYKFP
                     SGKYLDENALNCNLKIESGLKTSVRDGYMNLSGLENQVNKIINDNGDNTNQKEYISKL
                     VLDSVVRNMFKSLVYLCETYNVNEVVFAGGVSASKYILRELSMKLRKKHIEAYFTEPQ
                     YSTDNAVGCAIIGLNNFLGERV"
     gene            26374..27579
                     /locus_tag="PC414_000502"
     CDS             26374..27579
                     /locus_tag="PC414_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDF2462915.1"
                     /translation="MKLRALDISEANSYIKRILINDPILSNLKVKGEISNFKVHSSGN
                     VYLSLKDETSKLNCVIFKSNFNRNLKLDNGVKIIANGYISVYERDGAYQLYINEIEIE
                     GIGNLHIEFNRLKEKLNKEGLFDPKYKIPIPKMPNSIGVITSPTGAVIRDIINVIKRR
                     YPKVNIKLYPVTVQGDKSAEEICEAIRFFNHMKNVDTLIVGRGGGSIEELWSFNDEMV
                     AREVFNSQIPIISAVGHETDFTICDFVSDMRAPTPSAAAEIATPSLDDINYKLGNIKS
                     RMSKSLTNQIELDQYRLETVFNKINNYLDSYTIKDKVIQLDKIYDKIIFGIENNLKLE
                     DEKLVKIGALLHNLSPLATMDRGYSITQKNGKVINSIKGLKIKDSIDIVLKDGNLECM
                     IDKIENKEG"
     gene            27581..27796
                     /gene="xseB"
                     /locus_tag="PC414_000503"
     CDS             27581..27796
                     /gene="xseB"
                     /locus_tag="PC414_000503"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDF2462916.1"
                     /translation="MNLTYEEAYKRLESILSELESKNASLDESLSLYEEGISLYKHCN
                     KLLDDAKLKISKFNQLGIEEDFKIEEE"
     gene            27799..28686
                     /locus_tag="PC414_000504"
     CDS             27799..28686
                     /locus_tag="PC414_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDF2462917.1"
                     /translation="MEFKQCLKEKASFVEKVLKEYMPKEEGYQKTVIEAMNYSLSAGG
                     KRLRPILTLEACKIVGGNEDEAIPFAIAIEMIHTYSLIHDDLPALDNDDLRRGRPTNH
                     KVYGEAMGILAGDALLNYAFEVMLAGSINKENPEKYLKAINEIAKGAGIYGMIGGQVV
                     DVESENKQIGKEKLDYIHMNKTAAMMVGCMRAGATIGGANSEQMEEITKYAKNIGLSF
                     QIVDDILDIVGDEAKLGKKVGSDIENHKSTYPSLLGLDKSKEIAHNLIDEAKKSIEKL
                     SDDVDFLKGLAEYIIDREY"
     gene            28716..29153
                     /locus_tag="PC414_000505"
     CDS             28716..29153
                     /locus_tag="PC414_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HDF2462918.1"
                     /translation="MDFFSEIFNNGALGISLIACFLAQFIKIFTGKEKRIELSRILIS
                     GGMPSSHSSFVTSLATVVGIEKGFNSTDFAIITVLALIIMYDAAGVRRAVGKQATILN
                     QMVADIQHGKHIEQKKLKELIGHTPLEVWFGALLGIVTALILM"
     gene            29187..31052
                     /locus_tag="PC414_000506"
     CDS             29187..31052
                     /locus_tag="PC414_000506"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HDF2462919.1"
                     /translation="MYKYLDKVNSPKDIKNMSIEEMDLLAKDIRKFLVKSVSKTGGHL
                     ASNLGVVELTLALHKVFDSPKDKIVWDVGHQSYVHKIVTGRKDCFVSLRQFNGLSGFP
                     KENESPHDIFDTGHSSTSISIATGIACARDIKKENYSVISVIGDGSITGGMALEALNQ
                     LGYIDTNMIVILNDNEMSIDKNVGGMSKYLSSIIRNSTVEKMTDEVDKILNVTQTGEI
                     LSKTAHRFKDKLMYSFSPQDCSFFDSLGIRYYGPIDGHNTKELIDILRKAKHKKGPVL
                     LHVITKKGKGYRFAEEQPDKYHGVSKFDIKTGVTSAKVKSMSISVGEKLVDMANSNEN
                     IVAITAAMPSGTGLNLFESAYPKRYYDVGIAEQHATGFAAGLAKNGMKPYFAVYSSFL
                     QRAYDQVIHDVCITKKPVTFLIDRAGLVGNDGETHHGMFDLSYLNSIPNIVVMAPKDT
                     REMELMMDLSLKLDCPLAIRYPRGSSYYLDKGEYGEIVLGKYEVLDDGQDTVILCIGS
                     MVKHALEAKEILSREGINPTIVNARFLKPIDEGMLKALLKNHKNVVTIEDNIVTGGFG
                     SRINKFIIDNEYNVNILNIAIPEEFVKHGNIDELYDFVGLSPKSIADKIRKLVIE"
     gene            31185..31997
                     /locus_tag="PC414_000507"
     CDS             31185..31997
                     /locus_tag="PC414_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family rRNA
                     (cytidine-2'-O)-methyltransferase"
                     /protein_id="HDF2462920.1"
                     /translation="MKKRIDLLLVEQGYFESRERAKKAIMAGLVFVDNQRCDKAGTEV
                     KEDCSIEVKGNPIPYVSRGGLKLEKAMKNFDLTIDGKVCMDIGASTGGFTDCMLKNGA
                     IKVFSIDVGYGQLAWKLRQDDRVVCMERTNIRNVTIEDTKQFADFASIDVSFISLKLV
                     LPKAKELVRHDGEVVALIKPQFEAGREKVGKKGVVREKSTHIEVIKMISDFSVENGFE
                     ILGLDFSPIKGPEGNIEYLIHLRNGNEGYEFDGETYNNKIVEVVEASHNLDK"
     gene            32017..33696
                     /gene="recN"
                     /locus_tag="PC414_000508"
     CDS             32017..33696
                     /gene="recN"
                     /locus_tag="PC414_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HDF2462921.1"
                     /translation="MILELYMKNCALVEELRLNIDKNLNILTGETGSGKSIIIDALGL
                     CLGDKYDRSFLRKGTDKGLVEAVFFSDNRYLKKILEENDISMEDDNLLVITRLIYSDG
                     KSTARVNGRTVKVSLLKEIASTLIDIHGQHQNQALFNKDTHLKFLDLFGENELEEFKI
                     AYKNVYHKYSEVKKALNCLTENKDEMQIQREIDLLRFQINEIEAANLNKNEYEDLLKQ
                     REVYRNSEKIYNNLNSSYSKLHNGEYNVIDLIGLASKELNDISKYDSVLSEYSDTVER
                     IMYELQDISGEIRNYKDNIDFEPYELEQIELRIDEINNLRRKYGDSIDDIFEYYGKIK
                     DRLDEILNRDERVEQLRSKLMNIEEDLKIKASKLTKARIEVATTLEKILLDELKSLNM
                     KNVMFKVNFEKGPFTLDGVDDIEFMISFNLGEDIKPIYKVASGGEMSRFMLAFKTILA
                     DIDDIDTLVFDEIDTGISGIAAQIVGEKLSDIAKKKQIICITHLPQIAANADTHYCIE
                     KDTSNNRTFTNVSKLNESQRKNEIARLIAGNNITEKTIEHASEIIELAKKC"
     gene            33910..34776
                     /locus_tag="PC414_000509"
     CDS             33910..34776
                     /locus_tag="PC414_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2462922.1"
                     /translation="MNKKDTMHLIFTIVIFILLWYIVVAVSEINKNIESCSNKLDYLS
                     QEIMSTKSDISNTINEEMSKSYITKSIDLKVKKIEKKECVIDVDVQLSKLGKNGKVFF
                     MYKEDSDKWNETEMTKHGELSYACEIKINTGSEYDYKVVTSGAISESSDVQKLTKSDY
                     MPEQPIFNSGIRDNREYFIEIVENSNLFNHESEKSKNKVYDNIRLEKVDIIVNEGKKD
                     TIYKAKLAEGLDDGKTNNSNRNQVNYEVSFPKRDIDDIIYIKAKLTYNNGVEYTKDIT
                     EDITMGLQDLEK"
     gene            complement(34869..35360)
                     /locus_tag="PC414_000510"
     CDS             complement(34869..35360)
                     /locus_tag="PC414_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2462923.1"
                     /translation="MADKMAQNITLQFVEEKDLENLENVKLLFTEYSNSLNIDLCFQD
                     FNNELKTLPGKYKKPSGSLILAFVDENLAGCVALKKLEGKICELKRLYVRNQFRGLKI
                     GKILLEEIIEEAKKFGYTHMRLDTLPSMKSAQGLYEKFGFYDIEPYTYNPIEGARYME
                     LKL"
     gene            complement(35421..35861)
                     /locus_tag="PC414_000511"
     CDS             complement(35421..35861)
                     /locus_tag="PC414_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2462924.1"
                     /translation="MKLNVRIADINNWHDLVSLSVDKSQLDYIESNALSIAESKFITA
                     WVPVGIYDENSLIGFAMYGRLEDDRVWLDRFMIDSKYQGKGYGKASLDFLVNHLKNEY
                     NCDELYLSIFEDNKMAIKLYKDFGFEFNGELDYGGEKVMVLKSN"
     gene            36022..37080
                     /locus_tag="PC414_000512"
     CDS             36022..37080
                     /locus_tag="PC414_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HDF2462925.1"
                     /translation="MNKINKNFKNIWIILGAVSIFTMVIIFNKNCNVNKETQIEAFTI
                     KNHDKKFVYPMGNIIGVKANTDGVLVLGYEEEDVDYIGGIQIGDNIVKIDNKRIKNSQ
                     DVSEILNKIKKSKVEVTFERKNKYKTEVIETKKENGKYKLGLWVRDKISGIGTMTFYD
                     PSMEKFKAIGHAIKDSDTNELLKIKQGYIYKPEQLKIVKASNEKVGKIKGDFNDSNLM
                     GNFSNNSELGISGNITENHNKEFNVANKEKQLIEVGRPQDVKIGDAVILFEDKNKNIT
                     SYDIKIESIVYDKGNYRDMVIKVVDDKLLEYTGGIVQGMSGAPIIQNNKIIGAITHVF
                     RDNPKKGYGIFIDEMIKL"
     gene            37222..38046
                     /gene="spo0A"
                     /locus_tag="PC414_000513"
     CDS             37222..38046
                     /gene="spo0A"
                     /locus_tag="PC414_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HDF2462926.1"
                     /translation="MGGFLVEKIKIVLADDNKDFCQVLKEYLSNEDDIDILGIAKDGI
                     EALDLVKKTQPDLLILDVIMPHLDGLGVIEKLNTMDIPKMPKIIVLSAVGQDKITQSA
                     INLGADYYIVKPFDFVVFINRIRELVSNRVTQVEPKPRPVQETQMTRSDFVKNVGNIE
                     TEITNIIHEIGVPAHIKGYLYLREAIKMVIDNVELLGAVTKELYPSIAKKFNTTPSRV
                     ERAIRHAIEVAWSRGKVDTINQLFGYTVHNTKGKPTNSEFIAMIADKLRLEHSMVK"
     gene            38459..39580
                     /locus_tag="PC414_000514"
     CDS             38459..39580
                     /locus_tag="PC414_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2462927.1"
                     /translation="MRVEAPIKVDRKTKKLAKRVESGEIAVINHIDIDEVAANSLVEA
                     KIKLVINAAPSISGRYPNKGPGILTENNILIIDNVGEELFEELKEGETIEVVDGKIYR
                     KGKFLGAGEVLDKYEVSHQIKAAYENLAVELDRFIDNTIDYAKKEKGFILGEVEIPKV
                     KTNYANKHVLIVVRGQDYKEDLSTMLSYIEEVKPVLVGVDGGADALIEFGYTPDVIVG
                     DMDSVSDEALKKASEIVVHAYTDGRAPGLKRVEELGLDAVVFPAPGTSEDIAMLIAYE
                     YKAELIVAVGTHSNMIDFLEKGRKGMASTFLVRLKIGSKLIDAKGVNLLYRSKLKIKY
                     IWALIATALFPVLVVASLSPGVQQFIQLMQLKFRVLLQM"
     gene            39598..40473
                     /locus_tag="PC414_000515"
     CDS             39598..40473
                     /locus_tag="PC414_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HDF2462928.1"
                     /translation="MHINMKYYIVTIGAIFIALGIGMLVGFNLNNNQQLSEQQANIIN
                     DLDDKFNILKEKNDKLDSDLASVNRDYEEAVNFINKNVDKILAGSLNGKSIGIISANE
                     NDDYTKNIEDIINKSNGSIAFNIILKENITNPEKLKEMSTKLGTDVKNANDAVNYIID
                     TLKKEDASDILTYLQELDVIKFNFIGDTYLKYDSVVIAGGNDAKDSTKQFEKIEKFVV
                     SKLKSENKYLVEVQNTGVKTSYVELYSKNKVATIDNIDEGIGSISLAILLQQGNIVGN
                     FGRLDTATSLLPSIK"
     gene            40497..41189
                     /locus_tag="PC414_000516"
     CDS             40497..41189
                     /locus_tag="PC414_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2462929.1"
                     /translation="MDSYISIIIPAYNEESRIKYTLESILDIKQINEIIVVDDGSIDN
                     TAKVVSELENEKIKFFKLDKNRGKGYALNYGIKLAMKNADIIGFLDGDLGSSAKEVEK
                     LIVPILNGEADVTIAKFPPAKRKGGLGFVKGLAKNSVFEMTGVELDATLSGQRIFKRE
                     VLEKFDEIPFGYGVEVGMTIDILKYGFKIKEVLVNMTHNETGRNLKGFIHRGKQYYHI
                     KKVLGQKKKEWR"
     gene            41197..42021
                     /locus_tag="PC414_000517"
     CDS             41197..42021
                     /locus_tag="PC414_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDF2462930.1"
                     /translation="MKDYILYTILLLTGLFGTYAVIPLFKNLLINGNVLRPNYKKDMI
                     PVSMGIVFLPMIVINGIILAFFTTEFKNLAYIFMFMFGMISMFFAGILDDVIGNRDVS
                     GLKGHFKSLFKGSLTTGGFKALFGGFVGLIVSVAISKDIIDIIVNTLIIALSTNLMNL
                     LDLRPGRAIKAYLFIMVIVFLTLTGFVQVLPLLIVPNVLAYFNYDLKARAMMGDTGSN
                     VLGISIGMLISFGYPFNIRLGWLIFVVIMHIFTEKYSLTKIIEKNKLLNFIDKLGR"
     gene            42026..43093
                     /locus_tag="PC414_000518"
     CDS             42026..43093
                     /locus_tag="PC414_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HDF2462931.1"
                     /translation="MISKRVGIVESIVSQTETLDDIRVNINGEIQKAYNYPKISGTIN
                     IGDEVVLNTTAVELSLGTGGYHFVITNLNNIESTLTEGGHIMKLRYTPLQIKVDSVEE
                     QESIYHDKFANFKGLEGLSVVVGTLHSMLTPFAASFKRNNPNKKLVYIMTDGASLPIY
                     LSKNVDTLKEKKLIDSTITIGNAFGGDYECINIYTALITAKEILKADAVFVSMGPGIA
                     GTGTKYGFTGIEQGSILDAVKKLEGRAIAIPRISFADKRERHQGISHHSMTVFKEIVN
                     VNVDIPITIYDDEKLNFIKEQINLNGLDKKHNIIYIDNNKTKEDLDYFNLKVKSMGRN
                     YEQDEAFFKAASTAAYYLMEV"
     gene            43095..43631
                     /locus_tag="PC414_000519"
     CDS             43095..43631
                     /locus_tag="PC414_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDF2462932.1"
                     /translation="MVLEEKTISSDRVYTGKVITLKVDTVEIPGQGYQKRELVEVGGA
                     VGIVAITDDNKVVLVKQFRKPIEKPIFEIPAGKLEKNESPKECAERELKEETGYSAKN
                     IKLIHKFFTSAGFSNEIMFVYLATGLTPGENNLDADEFLDVYEIELEEAYNMVLKNDV
                     EDAKTSIGLLLVKDMFKN"
     gene            43699..44304
                     /locus_tag="PC414_000520"
     CDS             43699..44304
                     /locus_tag="PC414_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HDF2462933.1"
                     /translation="MRRTYRKNDFRELNRQIIIIGLLFMMSIVVGSYINKILPGSSNN
                     ILNNINPAVEYYNLNISIKDTVIQNLKSDAIFMGSIALLSLFVVTIPAVLVAFVLKGM
                     SIGYTINSCILALKFKSIKMILIILFKNLIIIPCAIILALISLSYFKEMVYEFKKKSR
                     KNMQFLIKRYILNIIIIIALSLGLQLILNTASIGIIKFLAK"
     gene            44316..45230
                     /locus_tag="PC414_000521"
     CDS             44316..45230
                     /locus_tag="PC414_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF2462934.1"
                     /translation="MDIIEGYIDYIKDKKKLSENTVASYFMDIKKYMEYINQKSIKLV
                     DIVENDIISYLIKLEKDNVSIATIARMISSIKSFHDYLFLNHICTNNPAKDIKKPKIK
                     KENINILTEEEIERLLNFPKLTTPKLIRDKAIFEVLYGTGIKVSELVEMNIEDIDLDI
                     DYIYCNSCCKNQRVIPLCDITKLYLEKYLKEARPKMAAEGEKSLFVSSLGQRFTRQGL
                     WKVIKKYSNLANIDKNINPTMLRHSFAIHLLNEGANIAVVSKILGNVNLSSLQVYLNH
                     IDKNVRREIKEKHPRNDVDVELQKAEAK"
     gene            45272..46444
                     /locus_tag="PC414_000522"
     CDS             45272..46444
                     /locus_tag="PC414_000522"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDF2462935.1"
                     /translation="MSRVIWIVIDSVGIGALPDAEKFGDSKDVSTLGNIFKEYPDIQI
                     PNMRNLGIGNIEGIDFFESIKEPIGCFGKCKEMSQGKDTTTGHWEMTGIIVDKPFKTF
                     EHGFSKEIIEEFEKKTGRKVVGNKPASGTVIIDEYGEHQIKTGDVIVYTSADSVFQIA
                     ANEEVIPLEELYNMCKIAREIMMGDNAVARVIARPFIGKKKGEFVRTSNRRDYSLDPF
                     EPTVLDNIKESGLDVLAVGKIEDIFNGKGITDAIHTKSNMDGVDETLNYMKQDNKGLI
                     YSNLVDFDSKYGHRRDPEGYKKALEEFDSRLPEIMANMREDDILIINADHGNDPTYKG
                     TDHTREYIPVMIYGNKIKKGFNLGVKDTFADIGATVADILNVKLPKHGSSFKEDLF"
     gene            46491..47303
                     /locus_tag="PC414_000523"
     CDS             46491..47303
                     /locus_tag="PC414_000523"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDF2462936.1"
                     /translation="MFEKIAQSSKFINSKSNIKPKIGLILGSGLGDLANDIEDPVTIK
                     YSEIPNFPVSTVAGHAGQLVIGKLEGKEVIAMQGRFHYYEGYSQKEATFPVRVMKELG
                     VEILIVTNAAGGVNKEFKAGDLMLIRDHINFSGSNPLVGKNDDRFGARFPDMSDAYSS
                     KYVDVVKNCAKECNIDVKEGVYMFFSGPNYETPAEVRMAQILGADAVGMSTVPEVIVA
                     SHSNIGVIGISCITNMAAGILNQPLSHEEVIETTQKVKSEFMNLVKTTVKNL"
     gene            47507..48832
                     /locus_tag="PC414_000524"
     CDS             47507..48832
                     /locus_tag="PC414_000524"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HDF2462937.1"
                     /translation="MRMYDIIKKKRDNLELSKKEINYFIENYSKGDIPDYQASALLMS
                     IYLNKMNKQETVHLTEAMMNSGDMINLSEIDGIKVDKHSTGGVGDKTTIALIPLVASC
                     GAPVAKMSGRGLGHTGGTIDKLESIPGFSTEMEISKFIDSVNKSKIAVCGQSAKVAIA
                     DKKIYALRDVTATVDNISLISSSVMSKKLASGADAIVLDVKTGDGAFMKTLEESFELA
                     KSMVDIGTGMNRDTIGIVTDMDEPLGFAVGNSLEVIEAIETLKGNGPKDLVELCEVLG
                     AYMLVLSKVAHDFEDGVEKIRESIKSGTAIQKLKEFIENQGGNKDVVDDYSLFKKSKY
                     KYPVKSTKSGYISKIKAEDVGVSAMILGAGRETKDDILDLSAGIILEKKVGDYVNEGE
                     VLAYMYYNDEQKLSLAKGRFIDSYSIVDSKIEKNKLIYGIVTKEEIKKF"
     gene            49045..50124
                     /gene="mltG"
                     /locus_tag="PC414_000525"
     CDS             49045..50124
                     /gene="mltG"
                     /locus_tag="PC414_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HDF2462938.1"
                     /translation="MNFKENRLKIAVLIIVILIILAGIFVFIQIGPYDKNNKKDVIID
                     VPSGASVGKISDILYENKLIKNELLFKLLVKVSNKAPSIKSGTYLLNQSYSNNDIISL
                     LVSGKIYQDGIKVTIPEGATSKEIIAMLVSKNLGDKATFENLIKKPQEFYDKFSYLKE
                     DGITSLEGFLYPETYYFNSKKQSEEDILSEMLKVFDSKYTDKFKKKQKELNMTLQEVM
                     EMASIIEKEAVLDKDRPIIASVFYNRLKVGMPLQSDATIQYIFEERKKIVTYDDLKID
                     SPYNSYKNKGLPPTPISNPGIKSIEAALYPEKTDYLYFVAKIDGGNNYSTNYQDHLKY
                     VKEYKEARDKQSKDTKATNKENTKK"
     gene            50296..50970
                     /locus_tag="PC414_000526"
     CDS             50296..50970
                     /locus_tag="PC414_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HDF2462939.1"
                     /translation="MSNIVNDLVEDYIRVTLKEKEGFLKDLEIYAEENSVPIIHKEVS
                     DLLKVLLKVQKPKRILEVGCAIGYSSIFFATIIGKDADIITVERNEKMIEKAKENIKL
                     AGFDNNITILEGDAEEKLSQVQGEFDLIFIDAAKGQYKLFFDLVIDKLKDGGLLVSDN
                     ILYKGMVAHDDFVVRRKKTIVKRMRKYLDYICNCDYLSTSLVPIGDGVALSYKESKRG
                     DVDGIK"
     gene            50957..52204
                     /locus_tag="PC414_000527"
     CDS             50957..52204
                     /locus_tag="PC414_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDF2462940.1"
                     /translation="MELNKVELLAPAGDLERLKIAITYGADAVYIGGEIFGMRSAAKN
                     FSKEDMAEGVAFAHERGKKVFVTVNIIPHNEDFLQLEDYLLELEEIGIDAVIIADPGV
                     LSVIKKVIPNMEIHLSTQANTTNYLSANFWYEHGIKRVVVARELSFDEIYEIRAKTPL
                     DMDIEAFMHGAMCISYSGRCLISNYMTGRDANKGSCAQSCRWQYHLVEEKRPGEYFPI
                     YEDERGTFFFNSKDLCMIEYIPELIKSGITSLKIEGRMKTAYYVATVVRAYRMAIDEF
                     YRDPENWKFNPMWMEELKKGSHRHFTSGFYLNKPTTEDQNYQSASYVRNYDFIGIVRE
                     TEDEDGLIVVEQRNKMCVGDEIEVMGPYKETMFTKIEEMYNEEGEAIESAPHPRQIVK
                     LKLSVKVGKDYMLRKVIEEKVEE"
     gene            52276..53946
                     /locus_tag="PC414_000528"
     CDS             52276..53946
                     /locus_tag="PC414_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HDF2462941.1"
                     /translation="MSKKKTPFLKKVGKRSWCIFTIILIIYSVLIYRLVDIQVLKGDK
                     YKQSVESQSVEKVELNSGRGIIYDRNNKKLTDTSKSQVLIVEKEKLNNNYKILELIKK
                     ATKMNDLDIYKAVQEQLTRPIIQIQTKNIDIDKSMKKELEKNGIMVEEKTMRYAKDGL
                     LSHTIGYIKEDDKSGQSGIEKSMDSVLRNSNEKYISAFKAGDAGNEKSLNILKGSVKT
                     VDNKDKDRHLKTTIDYNIQKKLEQILNKEENPTAAIISEASTGEILAMCSRPNFDQND
                     ISKSLKGKNGEFENRVIKATYPPGSVFKMVVLFSALENGVIDENYTYNCTGKTKVGNT
                     NEILRCNKRDGHGFQNLRQAFSNSCNPAFLDIAMKLGKEKILKSAEKLHLFEKVDIGL
                     DEEKIREAPKNISIRNLAIGQENIEFTPLQINQMTQIIANNGTFKPLYLYKSLVDNNM
                     NTIKTYKSSKKEELISPYVCTQVKEYMKSVSRTGTAKDLKDIEGGCGVKTGTAQSSLN
                     KKAIDHGWITGFYPEERPKYVITVLVEGTQKGNKSATPIFKEICESIK"
     gene            complement(54043..54327)
                     /locus_tag="PC414_000529"
     CDS             complement(54043..54327)
                     /locus_tag="PC414_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2462942.1"
                     /translation="MCNEISLLDILGTEANYVLDEVELKVQVGKLYEQLNKILTPRER
                     EIVQLRYGLTPYGYKTQREIAQKLDISRSYVSRIEKKALKKLEKELVQES"
     gene            complement(54350..55867)
                     /locus_tag="PC414_000530"
     CDS             complement(54350..55867)
                     /locus_tag="PC414_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF2462943.1"
                     /translation="MSVAIYLRKSRADEEAEKQGEFETLSRHKSTLLKLAKEQNLDVI
                     EIKEELVSGESIIHRPKMLELLKEVEENKYDAVLVMDLDRLGRGDMKDQGIILETFKE
                     SKTKIITPRKTYDLTDEFDEEYSEFEAFMARKELKLISRRMQRGRIKSVEEGNFIGTS
                     APFGYDAVTTGRKERILVPNKDADVVRTIFDLYINEDMGCSKISKYLNNLGIKTATGA
                     NWYNSAITNIIKNKAYCGYIQWQKKDYKKSKNPNKIKTVKLRPKDEWIEAKGKHEPLI
                     SEITWKKAQNILKKNGHVSYGNQIKNPLAGIVICKNCGRPLVYRPYADHDYIICYHPG
                     CNKSSRFEFIEAAILKSLEDTVKKYQLKASDIDLDKNNKDSNIEFQKRVLKGLETELK
                     ELGKQKNKLYDLLERGIYDEDTFIERSNNISSRTEEIKDSIKTVKNRLSTVKKDNSKI
                     IEDIKTVLSLYHDSDSLGKNKLLKSVIDKAVYYKSKEQKLDSFELMVHLKLHEDQ"
     gene            complement(55992..56813)
                     /locus_tag="PC414_000531"
     CDS             complement(55992..56813)
                     /locus_tag="PC414_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDF2462944.1"
                     /translation="MIISKKITAILVSFLIMISMVGCAPKESATEVAKKYLDSIEIKD
                     YEVAYNLLSKDSKESVSLEKFEEYQDTLFKLQTLKDYKLGEENKVEKYKYSNKEYTNL
                     VKIKETDTIKYITENKEDTANFDRYFIIEDNEYKLLWHKDFKRALSSNYVKIARSETE
                     KDEDKDYNKAAIDIKKAIEIDDKNPLAYYTQSYIYIELQRYDEALKSIDKYLEYLDKN
                     NEAYKIDASDAYNLRGVIFMSKSEFNKARESYNKALELNPDNEYAKTNLNDIKGL"
     gene            57004..58431
                     /locus_tag="PC414_000532"
     CDS             57004..58431
                     /locus_tag="PC414_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp26"
                     /protein_id="HDF2462945.1"
                     /translation="MKIKKLLTIGLSLSIFIASCPISVNALDKIESIKGADKYETAGI
                     IADKQNYTTAILINADSTMADGLSASGLAGAINAPILLTKKNNIPNATLKRLEKAKKV
                     YIIGGENSIDKYTETVLKGKGIEIKRLQGSDRIKTSYNVAKEINSINKVNKVILTNAF
                     KGEPDAMSAAPVAVRDKAAIVLTDGKSVPFNTTGVESYVIGGTSSMNDKIVKDTNSTR
                     LGGTDRYDTNKKIINKFYSGVKEFYIASGTDLVYALVGSTVAKNNAIVLVDNDSNKSV
                     LKSTTKLTAIGNLSDSILQQCLNVTKNIGDSNTGREYTVEDAANAVKKLLGVKKFYYE
                     PNYSEDEDLIFLLDKNFDFSGKEYYVFAYIKQGAEGDVRYCVAKNDMSKVYTYDVNGN
                     MKVCNPDDYKNKGSITEEQAKQIALKECAKRHKVSINNLVVDMIDFRDDIYSGNVYAV
                     KINSKNSNGTFGWFFIDIETGKVYN"
     gene            58581..58733
                     /locus_tag="PC414_000533"
     CDS             58581..58733
                     /locus_tag="PC414_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2462946.1"
                     /translation="MDNFLFNVLASLTASVVVYLISKLFKKAKSHSRTKSDLRVEFKF
                     IFKSKK"
     repeat_region   59387..59613
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=59387..59415
                     /rpt_unit_seq="gttttatattaactaagtggtatgtaaag"
     gene            complement(59900..60124)
                     /locus_tag="PC414_000534"
     CDS             complement(59900..60124)
                     /locus_tag="PC414_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2462947.1"
                     /translation="MDVNQRIKYIRENMNSSQKELLEKTNINTSVMNRIESGERPTRD
                     DGLIIFAKIFGLYTDYILGLSDTEKLNIGN"
     gene            60835..60990
                     /locus_tag="PC414_000535"
     CDS             60835..60990
                     /locus_tag="PC414_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HDF2462948.1"
                     /translation="MALDVKIEVKIIKDVVILNITHWKKETSLLTVKIARSQGDLSYV
                     KYFANES"
     gene            61236..61487
                     /locus_tag="PC414_000536"
     CDS             61236..61487
                     /locus_tag="PC414_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2462949.1"
                     /translation="MSSYFITRGDVMKILQQFKPYKYDKEKNIEYDEKIIANQNTEVY
                     IVYPRISDEENRRRWEEFNEVAKEVAINIAMKKVETVES"
     gene            complement(62697..63080)
                     /locus_tag="PC414_000537"
     CDS             complement(62697..63080)
                     /locus_tag="PC414_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2462950.1"
                     /translation="MRKKFFIGILIVLIISVIAILNIKNDKNVFIGESENWKAKFVTT
                     EGHSKLEIKYKNSKDVVEQKFNYSYQFIDGEDAVVVVKNEILEAGQEKAIKIQRPGLY
                     SNRESSYKLIISWNGNEETINLKKK"
     gene            63207..63551
                     /locus_tag="PC414_000538"
     CDS             63207..63551
                     /locus_tag="PC414_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2462951.1"
                     /translation="MNYRLKKSVTTILSLLIMTILLPTNASASKVEDSSMKVMYSEKQ
                     VTDMDKLYDMAKNGITDVKSSNEKGIIKNARTGESIYVDSISTTQLLEVKQSKNLTSK
                     IYSKTVFLILIQ"
     gene            63734..63949
                     /locus_tag="PC414_000539"
     CDS             63734..63949
                     /locus_tag="PC414_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2462952.1"
                     /translation="MHINQTGFISNELGIKQQQKKEPTSNSFSYNAPSSWKPVGRDSV
                     QYNCGFELIATLVRGGSSWAFNFIVQP"
BASE COUNT        25670 a         6643 c        11488 g        20256 t
ORIGIN      
        1 aaaagataga gttttattta gacatttatt acaaatcaaa atttattaca tattatttat
       61 aaaaaaatct aaggaggaaa taataatgaa aatattagta ttaaactgtg ggagttcttc
      121 attaaaatat caattaatag atatgaataa tgaagaagtt ttatgtatag gattggttga
      181 aagaatagga atagaaggtt ctatattaaa acatgaaaaa gcaggtagag atgataaata
      241 cgttgttgaa caaccaatga aggaccacaa agatgcaata gcattagttt tagaagctgt
      301 tgctcatcca gaatttggtg cagttaaaga aatgaaagaa atagatgcag taggacatag
      361 ggttgtacat gctggagaaa aatttgcaac ttcagtagta ataactccag aagtagaaga
      421 agctttaaaa gaatgtatag atttagctcc acttcataat ccagcaaaca taatgggaat
      481 agatgcttgt aaagctatac ttccagatgt accaatggta ggtgtatttg atactgcttt
      541 ccatcaaaca atgcctaaat cttcatactt atatggttta cctcatgagc tatatactaa
      601 atatggggta agaagatatg gattccacgg aacttctcat aattatgtat ctcaaagagc
      661 agcagagata ttaggaaaag atataaaaga tttaaaaata gtaacttgtc acttaggaaa
      721 tggtgcttct atagctgctg ttgatggtgg aaaatgtgta gatacatcta tgggattcac
      781 tccattagaa ggattaataa tgggaactag atgtggagat atagacccag ctatattacc
      841 attcttaatg agaaaagaag gtttagatgc tgatggattg gataaattaa tgaataaaga
      901 atctggagta tatggaatga ctggaatttc tagtgacttc agagatatag aagatgcagc
      961 taaaaatgga gacgaaagag ctcaagctac attagaagcg tatgttaaaa aagtacaaaa
     1021 atatataggc gcttatgctg ctgaaatgaa tggattggat gttgtagtat ttactgcagg
     1081 cgttggtgaa aatggaaagg ctataagagc agatattgct tctaacatgg agtttttagg
     1141 aatgaaatta gataaagaag ctaatgatgt tagaggaaaa gaaacagtaa tatctactgc
     1201 tgattctaaa gtaaaaatgc ttttaatacc aactaatgaa gaattaatga tagctagaga
     1261 tacattaaga ttggtaaaat aattaaatta aacctatgcc tatcaagtaa aaatacttga
     1321 caaacaaatg ctagatttgt ataataaatt tggtgatatg ttgaggtgaa cttaatgaaa
     1381 gttagcatag aaaaaataaa tagaaaagaa actgacaaaa tagacttgaa tttttgtgaa
     1441 aaaattgata ctattagtta ttgtgatgag atttacaaat tagtatcacc tgtaaattta
     1501 aaagggaaag tgtcaaagac taataaaggt ttgtatctag atatagatgt caattttaca
     1561 attgttgaca attgttcaag atgtcttaaa gaagtagaaa taccactaga gtattctata
     1621 caaggttttt tagtaaaaga agaagattat gatgaagatg agtttgagga atttgatcct
     1681 tttatattcg atggtgaaga aatcgacctt attgatataa tagaacagac attagatttt
     1741 aatgtacctc ataaagttct ttgtagtgaa aactgtaaag gtctttgtca agtatgcggg
     1801 gcaaacttaa atgaagaaga gtgttcttgc agtgaaatta caaacgatga ggaatacata
     1861 gatcctcgtt ttgctaaatt aaaagattta tttaattaaa ttttgactaa ggaggtggcg
     1921 taaatggcag taccaaagcg taaaacgtct aaatcaaaca caaaaatgag aagagcagct
     1981 aactcaaaga tggaagcaac aggatttgta agttgcccac aatgtcatga gccaaaatta
     2041 ccacatagag tgtgtccaga ctgtggatat tataaaggta aagaagttgt gtctaagtaa
     2101 tagcttctaa aaagcatgta gagtatactc tacatgtttt ttttgtacat ataaaaaaat
     2161 aagataattt actagagaaa ataagtggat tttatgcata ttaaaaaata gaaaaataat
     2221 aatataagta ttgaaaattt ttgtgtgtta ctatatacta ctattagtag ctggtactaa
     2281 ttacagctat aaaatttgga gggaacatga aaaagaaaag taaagcccaa agacaaaaag
     2341 agcttataga tatgctaaag acagatcctt tttatactga tgaagaatta tcaagtctgt
     2401 ttgatgtaag tatacagacc ataagattag atagaatgag tctaaacata ccagagctaa
     2461 gagaaagagt caaatcaata gcagaaactc agagttcaaa agtaaagact ttaggagtaa
     2521 aggaaattac aggagaaatc attgatttat ctgtagggcg attaggtata tctatgttag
     2581 aagttacaca ggacatgatt tactctaaga ctaatacttt aaaagatacg tatatttttt
     2641 ctctagcaga ttcattggca atggctataa tagatgcacc taaggttatt atgagagttg
     2701 caaatgtaaa aagttttaag ttgatagaac aacaagatag gcttatagca aaagcagaag
     2761 tgtatagaaa tatagataaa aagcattatg taaaagttgt tatcaacaat aaagcccaag
     2821 agcaaatatt tagaggaaag tttatctttg aagaattaga ttaggaggga aattatgaaa
     2881 atagtaattg atggaatggg tggagataat gcacctaaat ctaatgtaga aggcgcagta
     2941 aatgctataa aagaatatca ggttgattta ataatcacag gagataaaga tttacttgaa
     3001 aaggaatttt caaattatga atttgataga aataaattag agatagttca tactactgag
     3061 attatagaaa acgaagataa accagtaaag gctataagaa gtaaaaaaga ttcatctatg
     3121 gtagtagcat taaatcttgt aaaagaagga aaggcagatg ctataatatc agctggaaat
     3181 acaggagcct tacttgctgg tggattattt gttgttggta gaataaaagg aatcgataga
     3241 ccttgcttat gttcagcaat accaaatgta aagcgaggaa tgacacttat agcagactgt
     3301 ggcgcaaatg cagattgtaa acctaagaat ctagttgaat ttgcagctat gagtaatatt
     3361 tattcaagaa aagttttagg tcttgaaaat cctaaggtag cattagcaaa tgttggacta
     3421 gaagaaggca aaggcaatga tttagtcaaa aggtcatatg aagaaattaa aaaattagat
     3481 ttaaatttta tagggaatgt agaagctaga gaagttataa atgcatacac agatataatc
     3541 atatgtgatg gatttacagg taatatatta cttaaatctg ctgaaggtgt agcactttca
     3601 gttatgagtc ttataaagga aacttttatg gccagtacaa aaagtaaaat aggagcatta
     3661 cttataaagg atgatttaag aaaattaaaa agttttatag attattctga gtatggggga
     3721 gctccacttt taggtctaaa tggaggagtt ataaaagctc atggtagttc tgatgctaaa
     3781 gctataaaaa atgctataaa tcaaggaata aaattttcaa agggaaaagt cgttgaagat
     3841 attaaccaat ttataagcaa atacaatgaa gaaaataaaa ataacgaaga tgaataaact
     3901 cgttttggag gtttaatata taatgaatac aaaggctgga atattaggag taggaagtta
     3961 tctacctgag caatcatatg ataattttca cttcgaaaaa ataatggata catcagatga
     4021 gtggataagt actagaactg gtattaagga aagaagattt gccaaagagt ctgaagcaac
     4081 ttctgactta gcatctaaag ctgccttaaa agctatagaa tgtgcaaagt taaacgtaga
     4141 ggatatagag ctgattatat tagctacaat tactcctgat atgtctttgc cttcaactgc
     4201 atgtatagta caagatgcaa taggagcagt aaatgctact gcatttgata tatcagcagc
     4261 ttgttctgga tttgtatatg gagtaactat tgcaaagcaa tttgtggaaa caggttgcta
     4321 taaaaatgtg cttgtaatag gagcagaaac ttgttctaag tttttaaatt atgatgatag
     4381 aacaacagca gtattgtttg gagatggagc tggagcagct gttattggcc ctgtaaatga
     4441 gggtggaata ctgtctacac atatgggctc tgatggtaaa ggaaaagatt gtttaaaagt
     4501 acctgctgga ggttcaagac ttaaggcaag taaagagact gtagaagcta atctacatac
     4561 aatagaaatg gcaggtagtg atgtgtttaa atttgcagtt agaaagatgg cagaaacttc
     4621 tttaagagct cttgaaaaag caaatctaaa tacaactgat atagactact tagtgccaca
     4681 tcaagctaat ataagaatca tacaagcttc ttctaagaga ttagagcttg atatgaaaaa
     4741 agtttatgta aatatagaca aatatggaaa tatgtctgct gcatctatac cagtagcatt
     4801 agatgaagct tatagagaag gtaaaattaa aaagggtgat aatgtagttt tagttggctt
     4861 tggtggaggt ctaacttggg gggcttcagt tgtcaagtgg actttatagt ataaaatatc
     4921 taaaatgatt taattaacaa aattaaactt atacacaaaa taaagttgat taataataaa
     4981 aagtttactt ttatgatttc aagtgaagga gaattgtatg aataaaattt gcaaaatatt
     5041 aaatataaaa tacccagtta tccaaggggg aatggcatgg gtagctactg catcattagc
     5101 aagtgctgta tctaatgcag gaggacttgg cataatagca gcaggaaatg caccaaaaga
     5161 agctataaag aaagaaattg ttgagtgtaa aaaattaaca gataagcctt ttggagtaaa
     5221 tgtaatgctt atgtcgccat ttgttgatga tataattgat ttgattatag aagaaaaagt
     5281 tcaagttatt actactggtg ctggaaatcc tgcaaagtat atggatagat taaaggaagc
     5341 tggaacaaag gttattcctg tagtacctac aatagctttg gcacaaagaa tggaaaagct
     5401 aggagctaca gcagtaatag cagaaggtac tgaaggtgga ggacatatag gagaacttac
     5461 tactatggtc ttagttccac aagttgctga tgctgtaaac atacctgtaa tagctgctgg
     5521 aggaattgta gatggtagag gaattgcagc atcatttgca ttaggtgcca gtgcagttca
     5581 agtaggaact agatttattt gcagtgaaga gtgttctgtc cattcaaact ataaaaactt
     5641 agtactaaaa gcaaaagata gagatgcaat tgtaacagga agaagtactg gtcatccagt
     5701 aagaacatta aaaaataaac tatcaaaaga atttttaaag atggaacaaa atggagctac
     5761 tcctgaagaa ctggataaaa aaggtacagg agctttaaga tttgcaacag tagatggaga
     5821 catagaaaaa ggttcattta tggcaggtca aagtgctgct atggtaaaag aaataacacc
     5881 ttgtaaggaa attatagagg ctatggtaaa tcaagcaaga gagattatgc cagcaataga
     5941 actgtaaatc aggaggagca agatgggaaa agttgcacta gtattccctg gtcaaggagc
     6001 tcaatatgta ggtatggcca aggatttata tgaaaataat gctgttgcta aaagtgtaat
     6061 agatgaggct agtgatgcct taaatatgga tttaaaaaac cttatgttta atggaaatga
     6121 agaagaactt tctaaaactg aaaacactca acctgctatg gttacacata gtgtagcagt
     6181 attaaaagcg gtacaagctc aaatagattt aaaatataat gcatgcttag gattatcctt
     6241 aggagagtat agtgcgttgg ttgcagcaga tgcaattgac tttaaagatg ctgtatgttt
     6301 agttaaaaaa agaggtaagt ttatgcagga aacagttcca gcaggtgttg gagctatggc
     6361 agcagtttta ggtttagata gaaatgtact tgaaaatgtg gttaaagata tacaaggagg
     6421 tattgtagaa gtagcaaatt acaattctcc tggacaaata gttatatctg gtgaaaatga
     6481 aaagatagaa gaagcaatgg taaaatgcaa agaagcagga gcaaaaaggg cagttaaatt
     6541 aaatgtaagt ggaccttttc actcatcaat gttaaaagaa gctggtgtaa aattggccag
     6601 tgaattagaa aaagttaaca taactaaacc taaagttgat gtggtggcaa atgtaaatgc
     6661 agattactat aaaaacgaag gtaaagacct gcttataaaa caagtaagtt catctgttct
     6721 ttgggaagat tctatagaaa gacttttaaa tgatggatat gatactttta tagaaatggg
     6781 acctggaaaa actttaaaag catttattaa gaaaatagct tccaataaaa aagcaaatgt
     6841 caatatttat aatatagatg gtatagattc tcttaatgaa tttgtacaaa attatagaaa
     6901 tggagagata taatgataaa tcttacagga caagtagcag ttgttactgg tggttctagg
     6961 ggtataggaa aagaaatagc aaaaaaacta gcatcttttg gagctgatgt agtaatcaat
     7021 tatacttcta aagaagatga agcactaaaa actaaaaatg aaatagaaag tatgggggta
     7081 aagtgtacct ctataaaatg tgatgtgtct aaatttgatg aagtgaatca aatgatagat
     7141 tctgttgtaa gcgaatttgg aaaaattgat atattggtta ataatgcagg cataactaaa
     7201 gatggtctgc ttatgagaat gaaagaagaa gattttgata gagttataga tataaactta
     7261 aaaggtgtct ttaattgtac aaaagcagtt actaaaccta tgatgaaaaa gaagtatgga
     7321 agaataataa atatgacttc agtagttgga attatgggta atgcagggca aactaattat
     7381 tgtgcatcaa aagcaggtgt aattggattt acaaaagctt ctgcaagaga gttagcatca
     7441 agaaacataa atataaatgc agtagcacct ggatttatag aaacagatat gactaaagta
     7501 ctaagtgatg atgtaaaaga atcaacacta gcaaacatac caaagaaatc ttatggtaaa
     7561 ccagaagatg tagccaatgc cgtagcattt ttagttagtg acatgtcaag ttatataaca
     7621 ggacaagtaa taaatgtaga tggtggaatg gtaatgcaat aataatttaa aaagtagaga
     7681 aatctattaa aataaaaaat tttaggaggt acacaatgtt taataaagtt gtagaaataa
     7741 taatagagca attaggagta gaagataaag aaataactat ggaaacatca ttaatgaagg
     7801 atttagaagc agattcatta gatgcagtag aaataataat ggcacttgaa gatgaatttg
     7861 gtatagaaat accagataca gaagcagaaa acttcaaatc tataggagat atagttaagt
     7921 atatagaagc taataaatag tcgagcttta aaaaagtgga ggtatccgat gaataaaaga
     7981 gtagtaataa caggacttgg atgtgttacg ccgttgggaa caggaaaaga agagttctgg
     8041 agtaatataa aatctggagt atctggtata gataaaataa ctaattttga tgctagcaca
     8101 tatcaaactc agatagcagg agaagttaag aattttcatc cagaagaata tataagtaaa
     8161 aaggaattga agagattaga taaatttgct caatttgcaa tagtatctgc taaattagca
     8221 gttgaagatg caaatcttga tttagataaa gttgatagag aacgttttgg tgttataata
     8281 ggttctggaa taggtggagt tgaagctata gaaactcaac ataaaattct tttggaaaaa
     8341 ggaaataaaa gagtaagttc actttttgta cctatgatga taggaaatat ggcagctggt
     8401 caagtttcaa tatttttagg agcaaaagga cctaacacta atgtatgtac agcttgtgca
     8461 tcaggaactc attcaatagg tgatgctttt aaggtaattc aaagaggtga tgcagacctt
     8521 atggtagcag ggggttctga agctgctgta acaggtcttg cttttgctgg attctgtaac
     8581 atgaaggcta tgtcaactag aaatgatgac cctaaaactg catcaagacc atttgataaa
     8641 gacagagatg gttttgtaat gggagaagga gcaggtattg ttatcttaga agacttagaa
     8701 catgctttag caagaggtgc taaaatatat gcagaagtag ttggatatgg tttaactgct
     8761 gatgcatacc atatgacaac tccagctgaa aatggagagg gagcagctag gtctatgaat
     8821 atggctttga aagatggaaa tgttccatta gaagaagtag attatataaa tgctcatgga
     8881 acttcaactt attacaatga tttatatgaa actatggcta taaaaactgt atttggagaa
     8941 aaggcatatg acttatgtgt gtcatcaaca aaatctatga ctggtcactt attaggagca
     9001 tcaggtgcta tagaggctgt tgtttgtgct atgagtatag aagatagttt tgtgcctcca
     9061 actataaaca tacaagaagt tggtgaagat ttggatttag attatgttcc aaatcaaggt
     9121 aaagaaaaaa atattagata tgcactatca aattctttag gatttggtgg gcataatgct
     9181 acaatagtac taaaaaaata tgtataaata aatattaaat ataaaactaa aaaacaagca
     9241 cttgttttaa ctgtataaat ttatattagc ttaaatttaa agtctcctaa attattttag
     9301 gagatttttt tattaagatt taagtctagg atatgttgta aatcattttg atatattgta
     9361 ttattaatta ttaagaggtt atttatatat gcaagttttc attctacaat atacaaaagg
     9421 aggactaaaa ttttgaattt tagactacca aagaaaaggt tacccaaaaa gataattttt
     9481 atatgtataa tacttattag tattattttg tttataaagt atgaccaaaa tggattgaaa
     9541 gaaagagaaa ttataaataa gggaaaatat atgtctgaaa gccttgtggc agataaggag
     9601 tatgatgaag caaaaaaagt ggttaagact tcttttgaat ctattccaga aaaaaaatac
     9661 aataaagtat ataatgaagt ttgggatatg ttaaagacta tttcatatgt tccagatgga
     9721 acgaagatag tcattgattc tttagaaaag atgagagaat cagatgtgaa attatctgat
     9781 gaatgtagat ttaatataga aaaaagatta gcatcagtgt atataatgga taataattat
     9841 tctaaggcag tagatttaac tgtaaagtct ataaagttgg ctgagaaatt ggggaataat
     9901 tatgaaaaag caagactaga tgttgatttg gcgagcatat ttttaaagat aggtggctat
     9961 gaaacaggag agaaattaat aaaggattct tttaaaatag atattgatga aggtgaaaaa
    10021 aatggaatgg ttagattata tgctattata aatctagcag aaatatatgt agaagagggc
    10081 gaatatgata aagctattga aataagtagc aggataaaag attataaaaa gtttgtaaat
    10141 acggataatt acaatgattt tgatataatg gcctctataa tgcagtcaaa tgcgtatgtg
    10201 ggcaaaaata atatacaaaa ggcaaagact tttttagaaa aagctgatta tcttataaaa
    10261 tcagatagaa cagtttatat attagataaa gatatgtact attatatggc aatgggaaac
    10321 ttagaatata aaagcgaaaa ttataattct gcaatagata attataaaaa aagtttagag
    10381 atatctacta aaagaaagtt aacccaggag aaaataaaaa atttaaataa gatattaaat
    10441 atatatgaaa aacaaggaaa tatagaatct ttaaatgagt atcaaaagat gttaataaat
    10501 gaatataaag agaacaaaag tataagagat tcagagacta gtttctatat aattgataaa
    10561 gtatcaaacg aaagtaagct atttgaaaaa actaaaaaag aaataaaata ttataaaata
    10621 ttatttacag taattttaat tgctatatta gggtctactt ttttatacaa ccgattgaag
    10681 tattttaaag cacaaaattt acatgatggt cttacaaatg tttataatag aaaaagtttt
    10741 gatattatgt atgaaaagta cagagagaaa gatgataatt ttgcacttgt gatgatagat
    10801 attgataatt tcaagctcat aaatgataat tatggacata aatttggtga cactgttata
    10861 aagggaataa caaataccat ttgtgttatg ttagaacaag atgataaagt ctttaggtac
    10921 ggaggagaag aattttctgt attaataaga aataaatctg gtgatgaagt agcagatata
    10981 attgacagta taagggttgc aattgcaaat aaaaagtgga atgatgatgt gactgtgact
    11041 ataagtgcag gtgtagctca tgtttcagat agcaaagatg tactagaaga agctgataaa
    11101 aatttatata aagcaaaaca acttggtaga aataaagtag tatataagta attatatact
    11161 atataaataa gaggtataaa tatgaaacaa tcaatagcta tagtaactga taaaataact
    11221 aaactatcat ctttcttaga agaaaatata cgtttggttt taggtaatta tattgatata
    11281 aaccactatt atctggaagt tttaaaagag aatgataaaa tacaaggtga ttttgtacta
    11341 gtaatgaatg atgatagatt aaacagtatg agaaagcatt tagtacaaga tagtaagatt
    11401 atagtgataa ggagaacact taaagaaaat gaaatatatg gattattttc aataccaaat
    11461 ggaactgaag tattggttgt aaatgataca aaagaaacta ccttagaaac gataagctta
    11521 ttttacaaaa taggagtaaa aaatttaaag tttactccat atattgaagg aaaaatttat
    11581 aaaaatatag acatagctat aacacctgga gtaaagggaa aagtaccaag ttatataaat
    11641 aaagtaatcg accttggaaa tagatatatt gatatatcta cctttataga aataataaat
    11701 ggtcttagaa tagatgttaa agaaatacgt aaaaatttaa tggcatattc tgagagttta
    11761 ataagtttag ataatggagt taaagataat tataggcaac tgtttttaaa aatagaagaa
    11821 caagatgtta tattgaatct atcaaaagat ggaataatat ttacttcagc agatggtata
    11881 ataaacacat ttaataaaga agctttaaaa attttaggta taaatgaaaa tccacaaggc
    11941 aaaaacatag aagatatcat ttctgaagat ttaaatattt tacttgaaga caaagaaata
    12001 attgatgaag tggtatttac aaataaaaaa tatataaatg taaataaaaa aagtattttt
    12061 tctatgggga ataaagctgg gatatattat aacttacaag aaataacgta tataaaaaaa
    12121 ttagaacaaa atcttactaa taaattaaga gaacaaggtc agatagcaag atatacattt
    12181 aaagatataa aaactaaaaa taaaaatatg gtaaagtgtg tagaattggc taaaaaaata
    12241 tctaaatcag acctttctgt attaataata ggagaaagtg gtactggtaa agaattatta
    12301 tcacaatcaa ttcataatgc ttccaataga agtaatcaac cgtttatagc agttaattgt
    12361 gcagcagttc cagatagtct attagaaagt caactgtttg gatatgaaaa aggctctttt
    12421 acaggagctt taagagaagg aaaaaagggt ctatttgaac ttgctaataa tggaactata
    12481 tttctggatg aaattggaga tatgcctcca ttgctacaga caaagttact aagggtatta
    12541 caagaaaagc aggttatgcc tataggttcc cataatgtta taaacataga tgttagggtc
    12601 atagcagcaa caaacaaaaa cttactaaag atgattagag aaggaaaatt tagagaggac
    12661 ctgtattata gactaaatgt attgccaata gatgtaccat cattgaggaa tagaaaagaa
    12721 gatataataa cacttatgaa tttctttttg aaaaacaata taagactttc gccagaagtt
    12781 ataactattt tagaaaaata taattggcca ggaaatataa gagagttaca aaatgttgct
    12841 tcttatgtaa gtctaatgtg tgatagtatg gtttttagag atgacttacc accatatata
    12901 agggtagaat gtgctaacaa ttatgaagat ggatttggtt atacaagaaa agaatttgag
    12961 gaaatactga ggattctata tgaaagaaga gagtcatcct ctggaattgg aagagggttt
    13021 ataatcgaaa agcttataca ggaaggtatt gagatttctg aaagcaaagt tagaaaggtg
    13081 ctatcttatt taagtgaatg tggatacata tcatctaaat ctggtagagg aggaagtcaa
    13141 ataacctcag aggggataaa actttataat aatataaatt aagctaagta tttgatattg
    13201 aaatcacata ttttgaattt agatatttaa ataataataa acttttatat ttatagagat
    13261 taatttatat tatgtcagaa agaatattgt aatttataat taaatattaa taggttttta
    13321 aaataggatt agataggtaa taggttaagt tagaataacc cattacctat ttttatttgt
    13381 aagaaaagtg tacatagaat tagaagtaaa aaatacaact aataaaacta ttgaaaatac
    13441 tagataaata ataaataaaa ataattatag attttggcat acatattgct aattatagag
    13501 ataagacgac aaaataattg ggaggtttaa tgatgaaata caaaacaaca gggacttgtg
    13561 ctacagaaat tgaatttgaa gtaaaagaaa ataaagttac aaatgtaaac tttataggtg
    13621 gatgtgatgg aaatttaaaa gggctaaaag tattagttga aggcatgaat atagaagatg
    13681 taatacaaaa gttaaaagga atagagtgta aaacaaagcc aacatcttgc ccagaccaac
    13741 tgtctctagc attggaaaat tatatgaata taaagtaata cataaaacaa tcgattagtt
    13801 aggagtttaa ttatgaaacc aataataggg attttaggaa atttaataat aatggaaaat
    13861 ggtatgtttc caggtcttga aaggtcatat gtaaataatg attatataaa tgcagtgcta
    13921 aaaggtggcg gtagtccagt aataattcct gtaaatacag ataaagaagt tataaaaaaa
    13981 cagattgaaa tggttgatgg agtcttaata tctgggggat gggatatcaa tcctcaactg
    14041 tatggagaag aaacaagaga agaaacgaca tttatatatc cagaagtgga tgaatttgat
    14101 ttaattgcaa taagtatagc attagaactt aaaaagccta tattaggtgt atgtagggga
    14161 ctacaaattt taaatgtaag tttaggagga actttatatc aagataataa tcttatagaa
    14221 ggaagttata taaaacatac acaatcttct aaaagacatg tagctactca taaagttgat
    14281 gttaaagaag gaagtatttt agagggaatt ttaggaaaac aattacttac aaatagttat
    14341 catcatcaaa gtgtcaatca gcttggaaaa ggattaaaag ccatagcata ctcaaaagat
    14401 ggtataatag aagctattga gaaagaggat gaaaattttg tagtaggagt acagtggcat
    14461 ccagaaatga tggtagatta ctgtgataaa atggaaaaac tatttaaata ttttataaat
    14521 atttgttcga atttataata taccttgggg ggattgaaca tgaacgaaaa gaacaaaatg
    14581 ggattaataa gtattatact tttaggtatt aatgcagtag taggagctgg agtttttcta
    14641 ttaccaggag atgcaatgaa atcatttggt gtagcaagta tatttgtata tatatttgat
    14701 atgttgttag ttttatctat ggcattctgt tttgctgagg ttgcaggtaa attcaataaa
    14761 aatggtgctg cctatgtata tacaaaagaa gcttttggag atttttgtgg ttttgaagta
    14821 ggtcttatga aatgggtaat aggatgtatt tcttggggag ctttaatagt aggttttcct
    14881 acatcattat cagcagtatg ggctccagca ggtgaaccac atatacaaaa aattataatt
    14941 gtagctatga tagttgggct tacaattata aacttattag gtgtatctct atcaaaaatt
    15001 gtacaaaatg ttataactgt aggtaaacta attccattaa tactatttat aggaataggt
    15061 atatttttta taaaaggagt taattttact tcttcaacaa tggtacctcc aggagctgga
    15121 gcaacagagt ttggagcagc agcactttta atgttttatt catttacagg atttgaatct
    15181 atagcagttg cagcagaaga tatggaaaat ccacaaaaaa atataccaat agcaattata
    15241 agtgtaatag taattgcatc aataatatat atattaaatc aagttgtatg tgttggtata
    15301 ctaggagatt cgttatctag tacatctaca ccagtggcag atgctgcaag aatatgcttt
    15361 ggaaatatgg gagctggatt ggttaccttt ggaacattgg tttctgttgg tggaatttgt
    15421 atgtgtggtg cttttgtaaa tccaagaagt tgtgtagctt tagctgatga taaaatgtta
    15481 ccgagaatat ttgcaagaaa agataaaaaa ggtacaccat atgtagcgat aatagctact
    15541 atgttaatca ctataccaat agctctatct ggaagttttg ctgaattagc agcaataagt
    15601 gcagttgcta gattcataca atacatacca acatctttat ccgtattagt gtttagaaag
    15661 aaaagaccag aattagtagg tacttttaaa actccttttg gagcagtgat tcctctaatt
    15721 gctgtatgtg ttggaacatg gttgttattc caagcatcta tgcatcaatt aattatggga
    15781 ttaggagctt tggcaattgg agtaccatta tactttataa tgaagtctta taatagaaaa
    15841 atctatggtg aaaatttaaa gaaaatagtt taattatgtt ataacaattt attgagttaa
    15901 aattagacat tggcagaaaa ttaaatacaa ataagtatct acaaggctat attaaaatat
    15961 tattaatttt ttattttaat atagccttat attgttatca ataaatgaaa aatattgtaa
    16021 caacagaaga acaatagtat tatgtttata gacaaaaaac ttattaggag gtaaatttat
    16081 gccaacaata acattaaaaa atggtgtaga cgtacttatt agagaaggtg taagagaaga
    16141 tgctcaaagc attatagatt tttataatga agtgggtgga gaaactcatt ttctgtcatt
    16201 tggaaaggat gaatataaaa tatctttgga ggaacaagaa aatgctatag aatcagcaaa
    16261 agcatctgat aattctgtta aactgattgc atttatagat ggtgaaatag ttgggattgc
    16321 aacgattgat tctaatcaaa aagcaaaagg aaagcatgtt ggagtattag gaattgttgt
    16381 aaaagaaaaa tattggggaa ttggattggg taaaaggctt atgttagacc ttatagaatg
    16441 gtgtaaatca aatggaataa ccaagaagat aactcttgtc actaatgaag aaaattataa
    16501 tgctatagga ttatacaaaa aagttggatt tgaagtagaa agcatactaa agaaagaatg
    16561 ttattataat ggtgtttata ctgatttaat aggaatgtca cttttattgg gaatataatt
    16621 agtgtacatt ttatctcgaa aaaagtatat taagtatggt ataattaaaa aagaatataa
    16681 cttaatataa ttttggagga cgaattatga aaaatctgtg taaaatatca gatgattatt
    16741 taaacagcaa gcttaagtat ttaaagttat tatcaaaaca atatccaagt atatcaaaag
    16801 caagtacaga aataataaat ttagaggcta tattaaatct tccaaaggga acagagcatt
    16861 ttataacaga tgtacatggt gaatatgaac catttgtaca tgtattaaaa aatggttctg
    16921 gtgtaataaa gagaaaaata gaagaattgt tttcaaatac aataagagac agcgaaaaaa
    16981 aaatgttagc aactcttgta tattatcctg aacaaaagtt agacctaata attaagcaag
    17041 aggaaaatat agatgatttt tatagaataa atatttatag acttatagaa ctttgtaaat
    17101 atgcttctag caaatatact aggtctaaag ttagaaaact gttaccagaa aattttaaat
    17161 acataataga agaacttttg catgagcatg ttaaaagtga acataaagaa gagtattata
    17221 aaagtatagt tgaaacaata gttgacatag gaatagctaa ggagtttata attgctattt
    17281 ctacagttat acaaaaattg gttgtagata gacttcatgt aataggagat atttatgata
    17341 gaggtccaag accagatata attgtcgata agcttattga acatcattgt gtagatattc
    17401 aatggggaaa tcatgatata ttgtggatgg gtgcagcctc aggtgaaaaa acttgtatag
    17461 caaatgcact gaggatatca gctagatatg cgaatttaga cattgtagaa gacatatatg
    17521 gaataaatct attgccttta gccacttttg ctatagaaat gtataaagat gacccttgta
    17581 aagaatttat tccaaaggtt aatgaccaaa gtgtaacaac aacagaaaag tctttaatgg
    17641 caaaaatgca caaagcaata agtataattc aatttaagct tgaaggtgag gttatcagac
    17701 gaagacctga atttgaaatg gaacatagac tcttgcttaa tatgataaac tatgatgaag
    17761 gtacgattaa tttaaaaggt aaaacatata aattaaaaga tacttattta cctacaatag
    17821 acaaaaaaga cccatataaa ttaactatgg aagaaagaaa tgttattgat aaattagtat
    17881 cttcatttag aggtagtgaa aaactacaaa aacatgtttc atttttgttt tctaaaggaa
    17941 gtatatattt aaaggctaac tcaaatttac tgattcatgg atgtgtaccg ttaaatgaag
    18001 atggaagctt tatgtctatg aatataatgg gaaaagagta taaaggtaag gctcttatgg
    18061 ataagatgga gtctctagca agagaaggat ttttctttaa agataaggca gaagaaaaac
    18121 tgtatggcat ggatattatg tggtatttat ggactgggaa atgttcatca ttatttggta
    18181 aagatgatat gactacattt gaaagatact ttatagctga aaaagagact cataaagaaa
    18241 ataagaaccc atattttaaa cttagagaaa atgagatggc ttgtaaaaga ttatttgaag
    18301 aatttgattt agaacttgat gaatctcata taataaatgg tcatgttcct gtggagagta
    18361 aaaatggaga gagcccaata aaagctaatg gtaaaattct cgttatagat ggtggttttt
    18421 ctagggctta ccaaaaaact actggtatag ctggatacac tttgatatac aattctagaa
    18481 ctttgcaatt agtatcgcat gaaccattta attctgctga agaagccatt gcaaatgaaa
    18541 gtgatatctt atcgacaact gttgtagtag agcataaggc aaagagaaaa atggtgagag
    18601 atactgatga aggaataaag atacaagagg aaatagaaga tttaaaacta cttttaatgg
    18661 cttataagaa agggctaata aaagaaatgt agatgttata gaggcacttc atgattgaag
    18721 tgtctttttg ttaatactta ttaaatatat tataaagatt aataatagta gtttatatta
    18781 tacttactaa gtgaatccaa gataaatggg ttcacttttt ttatttataa acattattaa
    18841 aaaaatatag atgttttggt tatattcctt gtcctttata aatagaatta tataaacaaa
    18901 ggaggaggac aagagatgaa taaactttct gatgaaataa taaactctct ttctacaact
    18961 ataagagaaa aggtagaaaa agtctcaaac aataatctga acatagaaga aattaggtta
    19021 cgctcacaaa aaccattgat actaaatgca aactctaaag attattttta caatcaaaaa
    19081 actatgacac ttgatttaaa tcaacaaaac tcatatgtag taacaagaga agatgtagag
    19141 caaacttttc aaataatatg taaatattca atacattcat ttatggatga cataaaaaaa
    19201 ggattcataa ctttgagagg aggtcataga gtagggttag ttggaaaagc aatagtagaa
    19261 gatggacaag tcaaaaatat aaagcatata tcatctttaa atataagggt atcaagggaa
    19321 attattggat gctcggataa gattttaagt catatcataa agggaaaaaa tcagattaat
    19381 aatactttga taatatcacc acctcaatgt ggaaaaacaa ctttaataag agatatagta
    19441 agaaatttaa gtaatggtaa tgaagattat ggatttaaag gtttaaaagt ggctttagta
    19501 gatgagcgta atgagatagc aggagcttat ctaggtgttc cacaaatgga tgtgggaata
    19561 aggactgata taatagaaac ttgcccaaaa gacttgggta ttacaatgct tttaaggtct
    19621 atgtcaccaa atgttattgt gacagatgaa attggaagtg aaaaagaaat aaaggcatta
    19681 tacacagcat taaatggagg tataggatta ataactactg tgcatggaga ttcaatagag
    19741 gatatacaaa atagaaaaga attaaataga cttctggata aagagttatt taaaaaggtt
    19801 ataattcttt ctgcaaaaag aggagcagga acaattgaaa aaatttatga tttagaagaa
    19861 aagagatggt attttgcaaa ttaaaataat tattatagct ttcttgatag gaagtagtta
    19921 tttgataggt gaacaaatat acaaaactta tactcgtaga cataaacaat taaacgattt
    19981 aataagagtt ttagaaattt tgaggatgga tttatctttt ggactttata cattagaaga
    20041 aatctttaat aggataggtg gaaataaaga gttttgtttt tggaaatttt tctatcaaat
    20101 atcagaagga cttcacaatg aacaaagtaa aactttggaa ataataatat cagaaaatat
    20161 tgatgtctta agtaaagaaa cttatctagg caataaagaa attgaggaac tgaaaaatct
    20221 aatacttact ttagggaaaa gcgatataga gtcacaacaa agaatgatag atttatctat
    20281 agaaaatttg aaaaaacaaa cctatgagac aaaggaagat ataaacaaaa aaggcgtatt
    20341 gtataaaaaa ttagtgacat tcataggtat aggtatatgt attatattga tttaatatgg
    20401 ggaggtttat tatggaaata tcattgatat taaaggttgc aggtgtcggt attctaatat
    20461 cagtattgaa tatgatattg gaaaaaacag atagaaaaga ttgggcaggt cttactacat
    20521 tagcaggtgt aataatcgta ttgggtatgg taataacaga aataagtgac ttgtttaata
    20581 ctgtaagaac aatgtttcaa ctttactaaa gaggagggag taatttggaa ataatgcaat
    20641 taataggaat tgcaatcatt tcaactacac tctgtttagt aattaaaaaa gatagaccag
    20701 agatagcaaa tttcatagct ataataactg gagtaataat actattgtca gttatgttta
    20761 agctaaattt tatagtagat agtatacaag atttggctaa caaggctaac atcccaacta
    20821 tgtatatatc attaataata aaattaattg ggatagcata cttaatggaa tttgcgatac
    20881 aactttgtaa agattgtggg gaaggaaaca ttgcttcaaa actagaattt ggaggtaaaa
    20941 taatagttat gtcaatgtct ttccctatac ttttgtccat tgtagaaatg gtggtgaata
    21001 taattccata gatgtgtatc ttggaatgaa tataagtata ttagagagct ttcgttgtat
    21061 tgaggtttat agagtgaaga gaggcatggt tgcttatgaa aaaaagattt ttaccaatga
    21121 ttataggttt tttatttact ttcttttttg taaatatctt tgctattatg atatttgcta
    21181 atgaagtacc aagtagtgaa gataaagaat atggtgaaac aaaaaatagc atagataagt
    21241 atatagatgg tcaattagac aagctagata taaatgagat tcaagactat ataaataaag
    21301 aaatagttat taatgatgtt aatttaaaat catttgtaaa agatttaatt agtggagaaa
    21361 aaaatatctt agatttattc aataaagatg gattgaagat attgatgttt gatgaattta
    21421 aagcaagttt aaaggtagta gcagtaattt tggtactggc tctgttatca tctattttaa
    21481 agagtctgga aaactctttt tcatcaggtg cagtaagtca aatagcaaca tatataatat
    21541 ttatcacaat ggtatcactt acacttattg gttttaaaga tgtattacag atttgctatg
    21601 atgcaataga ccatactgta ggtcttatgc aagttataat gccaatatta ataacatttt
    21661 tacttttaat tggattccca ataacgtcga ctactttgaa tcctatcttc ataggaggag
    21721 taacatttat aaatgtattt tttaaaaatt ttctatttgt atctattact gttgcatttg
    21781 gaattttaat aataaacaat ttatcaaaaa atataaggct aaagagattt ttctcctttg
    21841 taaaacaaat aaattatgtt tctattggag ctatgtttac tgtttactta ggtcttgtat
    21901 ccatacaagg attgtatgta accagttttg ataaattcag tgtaaagact gctaaatttg
    21961 ctataggtaa ttttattcct gttgtaggtg gatttgtatc tgactctgtg gatattttat
    22021 tatcttcatc tcagcttata aaaaatatat ttggaggaat aggacttatc ttacttgttg
    22081 gaatttgctt acttccagta ataaaaattt tatcagtaat agtagtgtac aaattagcag
    22141 caataattgt agagcctgtt ggagaggatg gaatatctaa ttttttaaat gaagtggcaa
    22201 atttaatgat aataatgcta gcatcagtaa ttgctataac agtaatgttc tttgtaactg
    22261 tagctatctt gacctcgata agtgtcgtaa gtcaagggta atttatatat cagtgaaaaa
    22321 taattacatt taatctaatg tttttagaga ggaggatgct atgttagagg gcataaaggc
    22381 atggatagtt agtgttttga taggagcatt tatagtaaat atagtagata tgattttacc
    22441 aagttctaag ataaaacctt atgtcaacct agttttaaac tttatgtttg tatttattgt
    22501 aataactcca gtagtaggat ttttttcaaa agatatgagt ttagaagata gaatattaaa
    22561 atctatggga aactacaaca agcagtatgt agatagtaca aatgctttgg caaaagagac
    22621 tggaaataat agtctatcca agggatatga ggatgggtta aaagaagtac ttaaattaaa
    22681 acttgatgaa tatggttatg atttagaaga tatagagctt aatggggcaa atataaataa
    22741 tataaaaata aaagaaaaga ataatagtgc aaaaagtaat aacagttcca atgaagaaaa
    22801 taaaaataat agtactaaaa gagaggaaga aaacataaac tctaatgaca aagaaaattc
    22861 taaacaagtc ttcaaaaaag gtacagagta tgggttgaac ctaaatgaag aaaaattaaa
    22921 aaacgactta ataaaggtgc tggatgtttc tatagaagat atacaaattg ataaatagga
    22981 gttgggagaa tgttgaaaaa cttaaatgac aaagataaga gaaaggtata ttcactaata
    23041 actatagcag gaatatgtgt ggtttcatta gtattattat cttgttttcc tagtagcaaa
    23101 agcgataaac aagttggaaa aacagagact aataaaaatg ctgaaaagca agtcactaaa
    23161 gaacaagaaa aagatgattt agagtcaaaa ttaacagcaa tactatctaa aattgatggt
    23221 gcaggagacg ttgatgtaat ggttacattt gaatcaagtg aagaaataca acctgctttt
    23281 aattcaaata atacaacaga aacaacagag gaaaaagatg cacaaggtgg agaaagaaca
    23341 gttacgactt ctagtgaaaa taaaacaatg ataacttcaa attcaagtga ccctgtagtt
    23401 ataaaaacta cagagccaaa gataaaggga gtgatagtgg tagcaagtgg agcaagtgac
    23461 ccaagtgtta aagagacact ttatagtgca gtacaaacat cattacaagt agctggtcat
    23521 caagtagaaa tatattctaa ataaatacat ataaccgggg ggataaatga atgaagttta
    23581 attataaggg aagaggattt gtaataataa ctttaactgc aatgttagtg gtagtaggaa
    23641 cagtgaatta tcaactaagt aagaaatctt tattagaaac atcaaaagag tttaaggcat
    23701 atgaagaagc acagttgcaa aaaaatactg atgagagtga tagtagtaag acagaggatt
    23761 ctagtaatgt agataaacaa gatggtaagg aaagtgctga tattgatata gttgatagca
    23821 aagctagtaa agtaaaagaa aaggcaactg agacaagtaa ggaaataaaa gctcaattat
    23881 catcagagaa aaatatgaaa aaggcatctt atattttaga tatgaaaatg aatagagaaa
    23941 aacaaagaaa tgagttagtt caagatttaa atgaaatgat aaataatcca tctacaacag
    24001 aagaatctag aaaagaagct tctaatatga aattaaatat agttaaaatt caagaaaaag
    24061 aacttcaaat agaaaacctt ttgagtacaa aagggtatga agaagcgtta gtttatataa
    24121 gcgataacaa agttaatgta gttgtaaatg aagctaaatt agaaaagaaa gatgcagcaa
    24181 aaatatttga tttagttgca gaacaagcca atgtgaagta tgaaaatatt aaacttacaa
    24241 ataataatag taataagtaa agtataaacc tcagaattaa ctgaggttat tttcttgcaa
    24301 agtagacttg tatttggtat tattatactt ataaaaatag ttccttaaaa tcaggaggta
    24361 agttttaata tggaagataa taaatttgga caagtaaaaa tatctaatga tgtaatagct
    24421 acaatagctg gtttagcagc attagaagtt gaaggcatag aaactacagc aacattaaca
    24481 gataagttgt taaaaaataa tggagtaaaa atacaaatcg aagaagagga tgttaattta
    24541 gatgtaatgg ttacgataaa gtatggaatg tcaataccag atacagcttt taaagttcaa
    24601 gaaaatgtaa aaaatactgt tgagacaatg acaggactga aggtttctca agtaaatata
    24661 cacattcaag gaatcagctt taagaaggat aaagttgata aagaagaagc aaaagcaact
    24721 aagaaaaatt aatattagcc ctctgaaaag tcagagggtt ttttgctggt aattgatttt
    24781 atatggataa ttaaacttaa aggagaattt tgattgatga aaaaagatag ggcacaaaag
    24841 agtacaacaa gagaatatat aatgaaactt atataccaaa taaatattaa taaagaagat
    24901 tttgaaactt tagaagataa agtagataat tttttaaaag ataattcaga acatattata
    24961 aatagatata aagaacttgc acttcaatac tcaaaaaata caaatttaaa gttagaagat
    25021 actgaaattg aagatgttat agacaaaaaa tatataaata cagtatgcaa agcattgaaa
    25081 gaaaatcatg acaaaataga tgagttaata aataagcatg ctaaaaactg gacagtagat
    25141 agaatgccaa aggtagatgt ttctatactt agattgtctg tgtgtgaaat actttattta
    25201 gacactccta ataaagtatc aataaatgaa gcagtagaac ttgcaaaaat atattgtgat
    25261 gataagtctc ctaaatttat aaatggtata ttgggaagtg ttgttgatga aattgggaaa
    25321 taatataata attggaattg atactagctg ctatactact tctatagcag ctatctcttt
    25381 agataagaaa gttatattca atgaaaaaat aatgttagaa gtaagggata attcaaaagg
    25441 tttaaggcaa agtgaagctg tatttcaaca tatcaataat ttagggattc ttagtgatag
    25501 gataaaatct tttaaagata agtttaatgt agaaggagtt tgctcatcaa aaaaaccgag
    25561 acctgttgaa aactcatata tgcctgtatt caatgtaggt cataattttg gaaaattatt
    25621 gtctagtata tatggttgta ggttttatga gactactcat caagagaatc atattgaagc
    25681 aagtttatta aatagtaaat taaaaaataa taataaattt atttctgttc atatgtctgg
    25741 tggtactaca gagatacttt taacaagcaa acaagatagt catcacaatg tatgtgatac
    25801 taatttaggt aaaattgcca aaataagtat taaaaaagat gataaaagta agttatacaa
    25861 taattttgga tataatatag atataattgg aggaagtaaa gatattagct ttggtcaact
    25921 tatagataga gtaggaatta agcttggata taaatttcct tcaggaaagt atttggatga
    25981 aaatgcttta aattgtaatc ttaaaataga aagtggtctg aaaacttctg taagagatgg
    26041 gtatatgaat ttatcaggat tagaaaatca ggtaaataag attataaatg ataatggaga
    26101 taatactaat caaaaagaat atatatctaa attggtatta gattcagtag ttagaaatat
    26161 gtttaaatct ttagtgtatt tatgtgaaac ttataatgta aatgaagtag tatttgctgg
    26221 tggagtttct gcaagtaagt atattcttag agaattaagc atgaagttaa gaaaaaagca
    26281 tatagaagct tattttacag aaccacaata ttcaactgat aatgctgtcg ggtgtgctat
    26341 aataggattg aataactttt taggagaaag agtatgaaac taagagcttt agatataagt
    26401 gaagcaaact catatattaa aagaatttta ataaacgacc caatactttc taatcttaaa
    26461 gttaaaggag aaatttctaa ttttaaggtt catagtagtg ggaatgtata tttatcactt
    26521 aaagatgaaa catcaaagtt aaactgtgta atttttaaaa gtaattttaa tcgcaattta
    26581 aaattagata atggtgtaaa aattatagct aatggatata tatcagtcta tgaaagagat
    26641 ggtgcgtatc aattatacat taatgaaatt gaaatagagg gaataggaaa tttacacata
    26701 gagtttaata gattaaagga aaaacttaat aaagaaggat tatttgaccc taaatacaag
    26761 atacctatac ctaaaatgcc aaactcaatt ggcgttataa catctccaac aggtgctgtt
    26821 ataagagata taataaatgt aataaaaaga agatatccga aagttaatat aaagctttat
    26881 ccagtaacgg ttcaaggaga taaatcagct gaagaaatct gtgaagcaat tagatttttc
    26941 aatcatatga agaatgttga tacacttata gttggtagag gtgggggttc gatagaagaa
    27001 ctttggtctt ttaatgatga aatggtagca agagaggttt ttaattctca aattccgata
    27061 atatcagcag ttgggcatga aacggatttt actatttgtg actttgtgtc tgatatgaga
    27121 gccccaacgc catcagcagc agctgagatt gctactccat cattggatga tataaactat
    27181 aaacttggaa acataaaaag taggatgagt aagtctctta caaatcaaat agagctagac
    27241 caatataggt tagaaacagt atttaataag ataaataact atttagattc gtacacaata
    27301 aaagataaag ttatacaatt agacaaaata tatgataaaa taatttttgg aatagaaaat
    27361 aatttaaaat tagaagatga aaaattagta aaaattggag cattactcca taatttaagt
    27421 ccattagcta ctatggatag aggttatagt ataactcaaa aaaatggaaa agtaataaat
    27481 agtataaaag gattaaaaat aaaggatagt atagatatag tattaaaaga tggaaattta
    27541 gaatgtatga tagataaaat tgaaaataaa gagggatagt atgaatttaa cctacgaaga
    27601 agcttataaa agattagaaa gtattttaag tgaattagaa tctaaaaatg ctagcttgga
    27661 tgaatcttta agcctatatg aagaaggtat aagcctttat aaacattgta ataagctttt
    27721 agatgatgct aaattgaaaa taagtaaatt taatcaattg ggaatagaag aagactttaa
    27781 aatagaggag gaataaaatt ggaatttaaa caatgtctaa aagaaaaagc aagttttgta
    27841 gaaaaagtac ttaaagaata tatgcctaaa gaagaagggt atcaaaaaac tgttattgaa
    27901 gctatgaact atagcttaag tgctggaggt aaaagattaa gaccaatact tacattggag
    27961 gcttgtaaaa tagttggagg taatgaagat gaagctattc cttttgctat tgcaattgaa
    28021 atgatacata catattcact aatacatgat gatttgccag ctcttgacaa tgatgattta
    28081 agaaggggaa gaccaacaaa tcataaagta tatggagaag ctatggggat tttagcagga
    28141 gatgctttgt taaactatgc ttttgaagta atgcttgcag gctcaatcaa taaagaaaat
    28201 ccagaaaagt atctaaaagc tataaatgaa attgccaaag gtgctggtat atatggaatg
    28261 ataggtggtc aagtagtaga tgtagaaagc gaaaataaac aaattggaaa agaaaaatta
    28321 gattatatac atatgaataa aactgctgca atgatggtag gctgtatgag agcaggagct
    28381 actataggtg gagcaaattc tgaacaaatg gaagaaataa ctaaatatgc aaaaaatatc
    28441 ggattatcat tccagatagt agatgatata ctagacatag taggagatga agctaagtta
    28501 ggtaaaaagg ttggaagtga tatagaaaat cataagtcaa catacccatc tcttttaggt
    28561 ttagataaat ctaaagaaat tgcacataat ttgattgatg aagcaaagaa aagtatagaa
    28621 aaattatctg atgatgtaga ttttttaaag ggattggctg aatatataat agatagagaa
    28681 tactaaaata tacagctaaa atcgaggagg gaaaaatgga ttttttttcg gagattttta
    28741 acaatggggc tttgggaata agtctgattg cttgtttctt agcacaattt ataaaaatat
    28801 ttactggtaa ggaaaaaaga attgagttat caagaatact tatttcagga ggtatgccta
    28861 gttcccatag ttcttttgtt acaagtttag ctacagtggt aggtattgaa aaagggttta
    28921 attctactga ttttgctata attaccgtgc ttgccttaat tataatgtat gatgcagcag
    28981 gtgttagacg ggctgttggt aaacaagcta ctatattaaa tcaaatggtg gcagatatac
    29041 agcatggaaa gcatatagag cagaagaaat taaaagaatt aataggtcat acaccactag
    29101 aagtttggtt tggagcttta cttggaatag taacagcttt aatattgatg taaattgaaa
    29161 atttacagaa taaaggtgag gataatatgt ataaatattt agataaagta aattctccaa
    29221 aggatataaa aaatatgagt atagaagaaa tggatttact tgcaaaggat ataagaaagt
    29281 tcttagtaaa atctgtttca aagacaggag gacacttagc ttctaatcta ggagtggttg
    29341 agttaacatt agcacttcat aaagtatttg atagccctaa agataagatt gtatgggatg
    29401 tagggcatca atcttatgtt cataaaatag ttactggaag aaaagattgt tttgtatctt
    29461 tgagacagtt taatggacta agtggttttc caaaggagaa tgaaagtcct catgatattt
    29521 ttgatacagg acatagcagt acatctattt ctatagctac aggtattgcc tgtgctaggg
    29581 atataaaaaa agaaaactat agtgttatat ctgtgatagg agatggttct ataacaggag
    29641 gtatggcact tgaagcttta aatcaattag gatatataga taccaatatg atagtaatcc
    29701 ttaatgataa tgagatgtcc atagataaaa atgttggagg aatgtctaaa tatctgtcaa
    29761 gtattataag aaactcaaca gttgagaaaa tgacagatga agttgataaa attttaaatg
    29821 ttacacaaac aggtgaaatt ttatctaaga ctgcacatag atttaaagat aaactaatgt
    29881 atagcttttc tcctcaggac tgttcatttt ttgattcatt aggtataaga tattatggac
    29941 ctatagatgg ccataatact aaagaactaa tagatatatt gagaaaagct aaacacaaaa
    30001 aagggcctgt tcttttacat gtaattacga aaaaaggaaa agggtacaga tttgcagaag
    30061 aacaacctga taaatatcat ggagtatcaa agtttgatat aaagacaggt gttacatctg
    30121 caaaagttaa gtctatgtca attagtgttg gagaaaaact tgttgatatg gctaatagta
    30181 atgaaaatat agtagcaata acagctgcaa tgccatctgg tacagggtta aacctgtttg
    30241 aaagtgcata tccaaaaaga tattatgatg ttggtatagc tgaacaacat gcaacaggat
    30301 ttgcagcagg tcttgcaaaa aatggtatga aaccttactt tgctgtgtac tcatcatttt
    30361 tgcaaagggc atatgaccaa gttattcatg atgtatgcat aacaaaaaag ccagttactt
    30421 ttcttataga tagagctgga ttagttggaa atgatggaga aactcatcat ggtatgtttg
    30481 atttaagtta cttaaattct attccaaata ttgtagtaat ggctccaaaa gacacaagag
    30541 agatggagct tatgatggat ttgtcattaa aattagattg tccactagca attagatacc
    30601 caagaggaag tagttattac ctagataagg gagagtatgg agagattgta ctgggtaaat
    30661 atgaggtatt agatgacggt caagatacag tcatactttg tattggaagt atggtaaaac
    30721 atgctttaga ggctaaagaa atattatcaa gagaaggaat aaatccgaca atagttaatg
    30781 ctagattctt aaagccaatt gatgaaggta tgttaaaagc attattaaaa aatcataaaa
    30841 atgtagttac tatagaggat aatattgtaa ctggaggatt tggaagtaga ataaataagt
    30901 ttattataga taatgaatat aacgtaaata tacttaatat agcaatacca gaagaatttg
    30961 taaaacatgg caatatagat gaattatatg attttgtagg attatcgcca aaaagtattg
    31021 ctgataaaat aaggaaatta gttattgagt aaaaatctaa tgtagatatt gtaaattaaa
    31081 atttatataa aatatagaat ttatcaatta gaataaatat caagtgtgtt acttcaagct
    31141 actttagtta tcattaaagt agcttttaac agaaaggtaa ttgtatgaaa aaaagaatag
    31201 atttattatt agtagaacaa gggtactttg aaagtagaga aagagccaaa aaagcaataa
    31261 tggctggatt ggtttttgtt gataatcaaa ggtgtgataa ggctggtaca gaagtaaaag
    31321 aagattgtag tatagaagta aaaggaaatc caataccata tgtcagtaga ggtggtttaa
    31381 agcttgaaaa agcaatgaaa aactttgatt tgactattga tggaaaggta tgtatggata
    31441 taggggcatc cacaggtggt tttacagatt gtatgctcaa aaatggagca ataaaagtat
    31501 tttctataga tgttggttat ggtcaacttg catggaaatt gagacaagat gatagggttg
    31561 tgtgtatgga aagaaccaat ataagaaatg tgacaataga agatacaaag cagtttgctg
    31621 attttgcgtc aattgatgtg tcttttatat cgttaaagtt agtacttcca aaggcaaagg
    31681 agttagtacg tcatgatggg gaagttgtcg ctttaattaa acctcaattt gaagcaggaa
    31741 gagaaaaagt aggtaaaaaa ggagtagtta gagaaaaatc tacacatata gaagttataa
    31801 aaatgatttc tgatttctca gtcgaaaatg gatttgagat tttagggtta gatttttctc
    31861 ctattaaagg tcctgaaggt aatatagaat atctaattca tttgagaaat ggaaatgaag
    31921 gatatgaatt tgatggtgag acatacaata acaaaatagt agaagttgtt gaagcttctc
    31981 ataatttaga caaataggtt aaaagggggc ttagatttga tcctcgaatt atatatgaaa
    32041 aattgtgcct tagttgaaga actaaggttg aatattgata aaaacttaaa tatacttaca
    32101 ggtgaaactg gttcaggaaa atctataata atagatgcac ttggactttg tttaggtgat
    32161 aaatatgata ggtctttttt aagaaaagga acagataagg gacttgttga ggctgtattt
    32221 ttttcagaca atagatatct taaaaaaata ttagaagaaa atgatataag tatggaagat
    32281 gataatttat tggtaataac taggttgata tactcagatg gtaagagtac tgctagagta
    32341 aatggaagga ctgttaaagt gtctcttctt aaagaaattg catctacact tatagatata
    32401 catggtcaac atcaaaatca agctttgttt aataaagata ctcatttaaa atttctagac
    32461 ctttttggag aaaatgaatt ggaagagttt aaaattgctt ataaaaatgt gtaccataag
    32521 tatagtgaag taaaaaaagc tttaaattgc ttgacagaaa ataaagatga gatgcaaatt
    32581 caacgagaaa tagatttatt aagatttcaa ataaatgaaa ttgaagctgc aaatctaaat
    32641 aagaatgaat atgaagattt attaaaacaa agagaagttt atagaaatag tgaaaagata
    32701 tataataatc taaattcgag ttatagcaag ttacataatg gtgagtataa tgttattgac
    32761 ctaataggtc ttgcatctaa ggaacttaat gacatatcta aatatgacag tgttttaagt
    32821 gaatatagtg atactgtaga aagaatcatg tatgagttac aagacatatc tggtgagata
    32881 agaaattaca aagataatat agactttgaa ccttatgagc tagagcagat tgagcttaga
    32941 atcgacgaga ttaataactt aagaagaaaa tatggagaca gtatagatga tatatttgag
    33001 tattatggaa aaattaaaga tagattagat gaaatattaa acagagatga aagagttgaa
    33061 cagttaagaa gcaaactgat gaatatagaa gaagatttaa aaataaaagc cagtaaattg
    33121 actaaagcaa gaatagaagt agcaactact ttagaaaaaa tacttcttga tgaattaaaa
    33181 agcttaaata tgaaaaatgt aatgttcaag gttaattttg aaaaaggtcc atttacttta
    33241 gatggagtag atgatattga atttatgata tcttttaact taggagaaga tattaagcct
    33301 atatataaag ttgcatcagg tggtgagatg tccagattta tgttagcgtt taaaaccata
    33361 ttggctgata tagatgatat tgatacatta gtgtttgacg agatagatac tggaataagt
    33421 gggatagcag cacagatagt tggtgagaaa ttgagtgata tagcaaagaa aaaacagata
    33481 atatgtataa ctcatttacc tcaaattgct gcaaatgcag acactcatta ttgtatagag
    33541 aaagatactt caaataatag gacgtttacc aatgttagta agttaaatga atctcaacga
    33601 aaaaatgaaa ttgccagact tatagcaggt aacaatataa cagaaaaaac tattgaacat
    33661 gcaagtgaaa ttattgagtt ggctaaaaaa tgttagtgaa aaacaagcat tttaataggt
    33721 gtaaagaatt tttatagaaa tgctttacat ctatttttta tgatgaaatt attttaaaat
    33781 atctagtgcc tatattgaag aaaagtagat ttaaaaatag aagaaaattt gtataaattt
    33841 gtaactttga aatttctaaa tgcttaagta taatattatt tattaaggta gtagtgaggg
    33901 ggacataaaa tgaataaaaa agatactatg cacttaattt ttacaattgt aatttttatt
    33961 ttattatggt atattgtggt agcagtaagt gagattaata aaaatattga aagttgtagt
    34021 aataaattag attatttgtc acaagagatt atgagtacta aatcggatat tagtaatact
    34081 ataaatgaag aaatgtcaaa aagttatata acaaagagta ttgacttaaa agtaaaaaaa
    34141 atcgagaaaa aagagtgtgt tattgatgta gatgttcaat taagtaaact tggaaaaaat
    34201 ggaaaagtat tttttatgta taaagaagat agtgataaat ggaatgaaac tgagatgaca
    34261 aaacatggag aattatcata tgcatgtgaa attaagataa atacaggaag tgaatatgac
    34321 tataaagtag ttacaagtgg agctatatct gaaagttctg atgtgcaaaa attgactaaa
    34381 tctgattata tgcctgaaca acccatattt aattctggga taagagataa tagagaatac
    34441 tttattgaaa tagtagaaaa tagtaatctt ttcaatcatg agagtgaaaa gtcaaaaaat
    34501 aaagtttatg ataatattag attagaaaaa gtagatatta ttgtaaatga aggaaaaaaa
    34561 gacactatat acaaagccaa attagctgaa ggcttagatg atggcaaaac taataattca
    34621 aatagaaatc aagtgaatta tgaagtaagt tttcctaaaa gggatattga tgacataatt
    34681 tatattaaag ctaaacttac atacaataat ggagtagaat atacaaagga tataactgaa
    34741 gatataacta tgggattaca agatttagaa aaataaatac atatttaaat aaatattcaa
    34801 atagatttgt tcaataaaat agagtataaa taaaactgta gtagttttac ttatactcta
    34861 ttaaaaaatt ataattttaa ttccatatat ctagcccctt caattggatt ataagtataa
    34921 ggttctatat cataaaaacc aaacttttca tataatcctt gtgcactttt catgcttgga
    34981 agagtatcta atctcatgtg tgtatatcca aattttttag cttcttcaat gatttcttcc
    35041 aatagtattt ttcctatttt taatcctcta aattgatttc ttacatataa tctttttaat
    35101 tcacatattt taccttcaag tttttttaaa gcaacacaac ctgccaaatt ttcatcaaca
    35161 aatgccaata tcaatgagcc tgaaggtttt ttatattttc ctggaagtgt ttttaattca
    35221 ttattaaagt cttgaaaaca taagtctata tttagagaat ttgaatattc agtaaataga
    35281 agttttacgt tttcaaggtt ttctaaatct ttttcttcaa caaattgtaa agttatattt
    35341 tgagccattt tatccgccat tatataaaca tgtttatata atgacttcac ctccttgata
    35401 tatgtatatt tttcttaata tcaattagat tttaatacca ttactttttc accaccatag
    35461 tcaagctctc cattaaactc aaatccaaaa tctttataaa gcttaatagc cattttattg
    35521 tcttcgaata tactaagata tagttcatca cagttatatt catttttaag atgatttaca
    35581 agaaaatcta atgatgcttt tccatatcct ttaccttgat attttgaatc aatcataaat
    35641 ctatcaagcc aaactctatc atcttctaaa cgaccataca tagcaaatcc tattaaagaa
    35701 ttttcatcat atattccaac aggtacccaa gctgttataa atttagattc agcaatagaa
    35761 agtgcattgc tttctatata atctaactga cttttatcta cagacaaact aactaaatca
    35821 tgccaattat ttatgtctgc aattcttaca tttaatttca agatattacc gcccttcaaa
    35881 ctttttgtta caatctaata ctatcatatt atgtattaaa aatcacttat taagatttta
    35941 aataaacttt ttaaatgaat aaattctcag gaaaaaggaa ttataaatac ataataatac
    36001 agcataggag tagatggata tatgaacaaa ataaataaaa atttcaaaaa tatttggata
    36061 atattaggag ctgtatcgat ttttaccatg gttataattt ttaataaaaa ttgtaatgtt
    36121 aataaagaga ctcaaataga agcttttaca attaagaatc atgataaaaa atttgtatat
    36181 cctatgggaa atataattgg agtaaaagca aatacagatg gtgtattggt cttaggatat
    36241 gaggaagagg atgtagacta tattggaggc atacaaatcg gtgataatat agtaaaaata
    36301 gataataaaa ggataaaaaa tagtcaagat gtatcagaga ttttgaataa gattaaaaaa
    36361 agtaaagttg aagtgacttt tgagagaaaa aataagtata aaacagaagt aatagagact
    36421 aaaaaagaaa atggcaagta taaacttgga ttatgggtaa gagataaaat atctggaatt
    36481 ggaactatga ctttttacga ccctagtatg gaaaagttca aagcaatcgg tcatgccata
    36541 aaagattcgg atactaatga actattaaag ataaagcaag gatatatata taagccagaa
    36601 caattaaaaa tagtaaaagc tagtaatgaa aaagtaggta aaataaaagg agattttaat
    36661 gacagcaatt taatgggtaa tttctcaaat aattcagagt taggtataag tggtaatatt
    36721 acagaaaacc ataataaaga gtttaatgta gcaaataaag aaaagcaatt aatagaagtt
    36781 ggaagaccgc aagatgtaaa aataggagat gcagtaattc tttttgagga taaaaacaaa
    36841 aatataacaa gctatgatat aaaaatagaa agtatagtat atgataaagg aaattataga
    36901 gatatggtaa taaaagtagt agatgacaag ttattggaat acacaggagg tatcgtacag
    36961 gggatgagtg gagctccaat aatacaaaat aataaaatta ttggtgcaat aactcatgtt
    37021 tttagagata atccgaaaaa aggatatggt atttttatag atgaaatgat aaaattgtag
    37081 gtgaggcatt aaaaaatttt attattttat caattatcta ggaggaatat aattttggag
    37141 tgtcgaatat gctttagagt agataattag gaagcaattg tgtaaaaagt ttagttttct
    37201 gtaataagaa gatgtttttt aatgggggga tttttagtgg aaaaaatcaa aatagtttta
    37261 gcagatgaca ataaggattt ttgtcaggta ttaaaagagt atttgtctaa tgaagatgat
    37321 atcgatatat taggcatagc taaggatgga attgaagcat tagacttagt aaaaaagaca
    37381 caaccggact tattaatact agatgtaata atgccacatc tagatggatt aggtgtaatt
    37441 gaaaaattga atactatgga tatacctaaa atgccaaaaa taatagtact atcagcagta
    37501 ggtcaagata agataactca aagcgcaata aatctaggag cagattacta catagtaaag
    37561 ccatttgatt ttgttgtgtt tataaacaga attagagagc tagtttctaa tagagttaca
    37621 caagttgagc caaaacctag accagttcaa gagactcaaa tgactagaag tgattttgtt
    37681 aagaatgtag gaaatataga gacagaaatc acaaatataa tacatgaaat aggagtacca
    37741 gctcatataa aaggatattt atatttaaga gaagcaataa aaatggttat tgataatgtt
    37801 gagcttttag gtgcagtaac aaaagagtta tatccaagta tagctaaaaa attcaataca
    37861 acaccaagta gagttgaaag agcaataaga catgcaatag aggttgcatg gagtagagga
    37921 aaagttgaca caataaatca attatttgga tatacggtac acaatactaa aggaaaacca
    37981 actaattcag aatttatagc aatgattgct gataaattaa gattagaaca tagtatggtt
    38041 aaataaacaa gacataaaaa gtaaggcttt ttaattaagg cattggctat aaatgcgtat
    38101 tacaagcagc gaaacggtat aaccactagg gttataccgt ttcgctattt taataaatat
    38161 aaaaattttc tttattattt gcttactata tcaatataat aattttatta tactatggat
    38221 atagtatgtg tctttacaag ttgtaaactg acagtggttt attttttaat ataaatattg
    38281 actttgatgc aggtaaactt tgtattttta agcgtattat ggaatatgtt aaataaaaaa
    38341 atgatgaaat atagtattgt aaatgccaaa gatgcaaaac aaacttaaaa catttatttt
    38401 attgttaagt aatgctataa tataatgtga ttttaataat gatagtggag gtttaaatat
    38461 gagagtcgag gcccctataa aagtagatcg aaaaaccaaa aaacttgcta aaagagttga
    38521 aagtggggaa atagcagtta taaatcatat agacatagat gaagttgctg caaactcttt
    38581 agtagaagct aaaataaaac ttgtcataaa tgcggctcct tctataagtg gtaggtatcc
    38641 caataaaggt ccagggatat taactgaaaa taatatacta ataattgata atgttggaga
    38701 agagttattt gaagaattaa aagaaggaga aactatagaa gttgtagatg gaaagatata
    38761 tagaaaagga aaatttttag gtgcaggaga agttttagac aagtatgagg tttcacacca
    38821 aataaaggct gcctatgaga atttagctgt agaattggat agatttatag ataatactat
    38881 agattatgct aaaaaagaaa aaggatttat acttggagaa gtagaaattc caaaagtgaa
    38941 aactaattat gctaataaac atgtattgat agttgtaaga ggtcaggact acaaagaaga
    39001 tttaagtact atgctttctt atatagaaga ggtaaagcca gtattggttg gtgttgatgg
    39061 aggagcagac gctttaattg agtttggata tactccagat gtgattgtag gtgatatgga
    39121 tagtgtaagt gatgaagctt taaaaaaggc tagtgaaata gtagtacatg cttatacaga
    39181 tggaagagca ccaggtctta agagggttga ggaattaggc ttagatgcag ttgtatttcc
    39241 tgcgccagga actagtgaag atatagctat gcttatagcc tatgagtata aggctgagtt
    39301 aatagtagca gttggtacac attcaaatat gattgatttt cttgaaaaag gaagaaaagg
    39361 tatggcaagt acttttttag ttagactaaa aataggttca aagcttatag atgctaaagg
    39421 cgttaatttg ttatacagaa gtaaattaaa aataaagtac atatgggcat taatagcaac
    39481 agcattattt cctgtgttgg tagttgcatc attgtcacca ggagttcaac agttcataca
    39541 actaatgcaa ttaaagttta gagtattatt acagatgtaa attttaggag gataaatatg
    39601 catattaata tgaaatatta tatagtcact ataggggcta tatttatagc tttgggtata
    39661 ggcatgttag taggatttaa tttaaataat aatcaacaat taagtgaaca acaagcaaat
    39721 ataataaatg atttggatga taagtttaat atcttaaaag aaaaaaatga taaacttgac
    39781 agtgacttag caagtgtaaa tagagattat gaagaggcag ttaattttat aaataaaaat
    39841 gtagataaga ttttagctgg ttcgctgaat ggtaagagta taggaataat atctgctaat
    39901 gaaaatgacg attatacaaa aaatatagaa gacataataa ataaatctaa tggaagcatt
    39961 gcatttaata taatattaaa agaaaacata acaaatccag aaaagttaaa ggaaatgtca
    40021 actaaactag gtacagatgt aaaaaatgca aatgatgcag ttaattatat aattgacact
    40081 cttaaaaagg aagatgcaag tgacatttta acatatcttc aagaactaga tgtaataaag
    40141 tttaatttta ttggagatac atatctaaaa tatgattccg ttgtaatagc tggtggaaat
    40201 gatgcaaaag acagtacaaa acagtttgaa aaaatagaaa aatttgtagt ttcaaaactt
    40261 aaatcagaga ataaatattt agtagaagtt caaaatacag gagttaaaac ttcttatgta
    40321 gaactatatt ctaaaaacaa agttgcaact atagacaata ttgatgaagg tataggaagt
    40381 atatctctag cgattcttct acaacaaggt aatatagttg gtaattttgg tagattggat
    40441 actgcaactt cattgttgcc atctattaaa tagaaataat tttaaggaga gaaaaaatgg
    40501 attcttatat aagtataata ataccagctt ataatgaaga aagtagaata aaatatacat
    40561 tagaaagcat attggacatt aagcaaataa atgaaataat agttgtggat gatggttcaa
    40621 ttgacaatac tgctaaggtt gtatctgagc tagaaaatga aaagattaaa ttttttaagt
    40681 tagataaaaa tagaggtaaa ggatatgctt taaattatgg tatcaagtta gcaatgaaaa
    40741 atgcagatat tataggtttt ctggatggag atttaggaag ttctgctaaa gaagttgaaa
    40801 aattaatagt acctatatta aatggtgagg cggatgttac aatagccaag tttcctcctg
    40861 caaagagaaa aggaggtttg ggatttgtaa aaggtttagc taaaaattct gtgtttgaga
    40921 tgactggtgt tgagttagat gcaactttat caggtcaaag aatatttaaa agagaagttt
    40981 tagaaaagtt tgatgaaata ccttttggat atggagtaga agttggaatg accattgata
    41041 tattaaagta tggatttaaa attaaagaag tattagtaaa tatgactcat aatgaaacag
    41101 gaagaaattt aaaaggtttt attcatagag ggaaacagta ttatcacata aaaaaggttt
    41161 taggacaaaa aaagaaagaa tggaggtaat atcttagtga aagattatat actttatacc
    41221 attttattat taactggatt atttggaacc tatgctgtaa ttccactatt taaaaattta
    41281 ttgataaatg gaaatgtact tagaccaaac tataaaaaag atatgatacc agtaagtatg
    41341 ggaattgtat ttttgcctat gattgtaatc aatggaataa tacttgcttt ctttactaca
    41401 gaatttaaga atttagcgta tatatttatg tttatgtttg gtatgatatc catgtttttt
    41461 gcagggatac tagatgatgt cattggaaat agagatgtaa gtggtttaaa ggggcatttt
    41521 aaaagtttat ttaaaggaag tcttacaaca ggaggtttta aagctttatt tggtggattt
    41581 gtagggctta tagtttctgt tgctatatca aaagacataa ttgatataat agtaaataca
    41641 ctaataatag ctctatcaac taatttaatg aatcttttgg atttaagacc aggaagggca
    41701 ataaaagcat atttattcat aatggtaatt gtatttttaa cattgacagg atttgttcaa
    41761 gttcttccat tacttatagt accaaatgta cttgcatact ttaattatga tttaaaagca
    41821 agggctatga tgggagatac tggttcaaac gtccttggaa tatctattgg aatgctcatt
    41881 tcttttgggt atccatttaa cattagatta ggatggttaa tatttgtagt tattatgcat
    41941 atattcactg agaaatactc attaactaag ataatagaaa aaaataaact attaaatttt
    42001 atagataaat tgggaaggta gtattatgat aagcaaaaga gtggggattg ttgaatcaat
    42061 agtaagtcaa actgaaactc tagatgatat aagagtaaat ataaatggag aaatacaaaa
    42121 ggcatataat tatccaaaaa tatctggaac tataaatata ggtgacgaag tggttttaaa
    42181 tacaacagca gtagaactga gcttaggtac aggtggatac cattttgtaa taactaattt
    42241 aaataatata gaatctactt taactgaagg tggacatata atgaaactta gatatacgcc
    42301 tcttcaaata aaagtggatt ctgttgagga acaagaaagt atttatcatg ataaatttgc
    42361 gaattttaaa ggattagaag gtctttctgt tgtagtaggg actcttcata gtatgttaac
    42421 tccatttgca gcttcattta aaagaaataa tccaaataaa aaattagtgt atataatgac
    42481 tgatggagca tctcttccaa tttatttaag taaaaatgtg gatactttaa aagaaaaaaa
    42541 attaatagat agtacaataa ctataggaaa tgcgtttggt ggagattatg aatgtataaa
    42601 tatatatact gcattaatta cagcaaaaga aatactaaaa gcggatgcag tatttgtcag
    42661 tatgggacct ggaattgctg ggacaggaac aaaatatgga tttacaggga ttgaacaagg
    42721 ctctattctt gatgctgtaa aaaaacttga aggaagagca attgcaattc caagaattag
    42781 ttttgctgac aaacgagaaa gacatcaagg tatttcacat catagtatga ctgtatttaa
    42841 agaaattgta aatgtaaatg tagacatacc aataactata tatgatgatg aaaaattgaa
    42901 ctttataaaa gagcaaatca atttaaatgg tttagacaaa aagcataata taatttatat
    42961 agataataat aaaacgaaag aggatttaga ctattttaat ctaaaagtaa agagtatggg
    43021 aagaaattat gaacaagatg aggcattttt caaagctgca agcacagcag catattactt
    43081 aatggaggtt taagatggta ttagaagaaa aaactattag tagtgatagg gtttatacag
    43141 gtaaagtaat tactttaaag gttgatactg ttgaaattcc agggcaagga tatcaaaaaa
    43201 gggagttagt agaagttggt ggagcagtag gaatagttgc aatcacagat gataataaag
    43261 ttgtgttagt taagcaattt agaaaaccta tagaaaagcc aatctttgaa attccagctg
    43321 gtaaactaga aaaaaatgaa agtccaaaag aatgtgctga aagagaatta aaagaagaaa
    43381 ctgggtatag tgcaaaaaat attaagctaa tacataaatt tttcacttca gcaggttttt
    43441 ctaatgagat aatgtttgta tatttagcaa caggtcttac tccaggagag aataatttag
    43501 atgcagatga gtttttagat gtttatgaaa ttgagcttga agaagcatat aacatggtat
    43561 taaaaaatga cgttgaagat gcaaaaacat ctattggatt attattagta aaagatatgt
    43621 ttaaaaatta atatttataa aatgaataac cctttgtcct aaagcatatt attttataaa
    43681 ggacaagggg gaattaaatt gagaagaaca tatagaaaaa atgattttag agaattaaat
    43741 agacaaataa ttattatagg attgttgttt atgatgtcta ttgttgttgg gtcttacata
    43801 aataaaatat tgccaggttc tagtaacaat atattaaaca atataaatcc tgctgtagag
    43861 tattataatc taaatataag cataaaagat acagtgatac aaaatttaaa atcagatgca
    43921 atctttatgg gctcaatagc tctattaagt ctatttgtag taacaattcc agcagtgttg
    43981 gtagcttttg tattaaaagg gatgtcaatt ggttatacaa taaatagttg tatattggca
    44041 ttaaaattta aaagtataaa aatgatttta ataatattat ttaaaaattt gattattatt
    44101 ccatgtgcaa tcatattagc attaattagt ttaagttatt ttaaagaaat ggtttatgaa
    44161 tttaaaaaga aaagtagaaa aaatatgcaa tttttaatta agaggtatat actaaatatt
    44221 ataattataa tagcactatc attggggttg caattaatat taaatactgc aagtattggt
    44281 attattaagt tcctagctaa ataaaggagt ttataatgga tattatagag ggatatatag
    44341 attacataaa agataaaaaa aaattatcag agaatacagt ggcatcatac tttatggata
    44401 taaaaaaata tatggaatat ataaatcaaa aaagcattaa actagtagat atagtagaaa
    44461 atgatattat aagttactta ataaagttgg aaaaagataa tgtatcaata gctacaatag
    44521 cgaggatgat atcctctata aaatcttttc atgattattt atttttaaat catatatgta
    44581 caaataatcc tgcaaaggat ataaaaaaac ctaaaataaa aaaagaaaat ataaatatac
    44641 ttacagaaga agaaattgaa agactattaa attttccaaa gttaacaaca cctaagttga
    44701 ttagggacaa ggctattttt gaagtattat atggtactgg tataaaagta tctgaattag
    44761 tagagatgaa tatagaggat attgacctag atattgatta tatatattgt aattcatgtt
    44821 gtaaaaatca aagagtgata ccactttgtg atattacaaa actttactta gaaaagtatc
    44881 taaaagaagc aagacctaaa atggctgcag aaggtgaaaa atcattattc gtaagttcat
    44941 taggtcaaag gtttacacga caaggtcttt ggaaagtcat aaaaaaatat tctaatctag
    45001 ccaatataga taaaaatata aatcctacaa tgcttagaca ttcatttgct attcatttgt
    45061 taaatgaagg tgctaacatt gctgttgtaa gtaaaatttt aggaaatgtt aatttatcta
    45121 gtttgcaagt atatttaaat catatagata aaaacgtgag aagagaaata aaggagaaac
    45181 atcctagaaa tgatgttgat gtagaattac aaaaagcaga agctaaatag actatatagc
    45241 aaattttata atttagaagg agcatattta tatgagcaga gtaatttgga ttgtaattga
    45301 tagtgttggg ataggagcat tacctgatgc tgaaaaattt ggtgatagta aggatgtaag
    45361 tacccttgga aatatattta aggaatatcc agatattcag attcctaata tgagaaattt
    45421 aggtatagga aacatagaag gtatagattt ttttgaaagt ataaaagaac caataggttg
    45481 ttttggaaag tgtaaggaaa tgtctcaggg aaaagatact actacaggtc attgggaaat
    45541 gactggtata atagttgaca aaccatttaa gacatttgaa catggattct ctaaggagat
    45601 aatagaagag tttgagaaaa aaactggaag aaaagtagta ggaaataaac cagcttcagg
    45661 aactgtaata atagatgagt atggagagca tcaaataaaa actggagatg taatagtgta
    45721 tacatcagct gatagtgtat ttcagattgc tgctaatgaa gaagtaattc ccctagaaga
    45781 actctataat atgtgcaaaa tcgctaggga aataatgatg ggggataatg cagtagctag
    45841 agtaattgct agaccattca taggtaagaa aaaaggtgag tttgtgagga cttcaaatag
    45901 aagagattat tcactagacc catttgaacc aacagttctt gataatataa aagaatcagg
    45961 acttgatgtt ttagcagttg gaaaaataga agatattttt aatggaaaag gaattacaga
    46021 tgctatccac actaaaagca atatggatgg agtggatgaa actttaaatt atatgaaaca
    46081 agataataaa ggtctaatat attcaaatct cgttgatttt gattctaaat atggtcatag
    46141 gagagaccca gaaggatata agaaagctct tgaagagttt gatagtagac ttcctgaaat
    46201 aatggctaat atgagagaag atgatatttt aataatcaat gcagaccatg gaaatgaccc
    46261 aacatataaa ggtacagacc atacaagaga atatatacct gttatgattt atggcaataa
    46321 gattaaaaaa ggttttaatt taggagtaaa agatactttt gctgatattg gagcaactgt
    46381 tgcagatata ctaaatgtaa agttgcctaa acatgggtca agctttaaag aagatttatt
    46441 ttaaactata aaatgataaa aacttagctt aataggatgg agagattgga atgtttgaaa
    46501 agatagcaca aagtagtaaa tttataaatt ctaaaagtaa tataaaacct aagattggtt
    46561 tgatattagg ttctggacta ggtgatttag caaatgatat agaagaccct gtcacaatta
    46621 aatatagtga aattccaaac ttccctgtat caacagtagc aggacatgct ggacaacttg
    46681 taattggaaa acttgaagga aaagaagtta ttgctatgca aggtagattt cattactatg
    46741 aaggatattc tcagaaagaa gctacttttc cagtaagggt tatgaaagaa ttaggagtcg
    46801 agattcttat agtaactaac gctgctggag gcgtaaataa agaatttaaa gcaggagatt
    46861 tgatgcttat aagagaccat ataaacttta gtggaagtaa tccattagta ggaaaaaatg
    46921 atgacagatt tggtgctaga tttccagata tgtcagatgc atattcttct aaatatgtag
    46981 atgtagttaa aaactgcgct aaagaatgca atatagatgt caaagaaggc gtttatatgt
    47041 tttttagtgg tccaaactat gaaacaccag cagaagtgag aatggcacaa attttgggag
    47101 ctgatgctgt aggtatgtct actgttccag aagtcatagt agcttctcac tctaatatag
    47161 gagttattgg tatatcttgt ataacaaata tggctgctgg catcttaaat caaccactaa
    47221 gtcatgagga agtaatagaa actactcaaa aagttaaatc agaatttatg aatttagtta
    47281 aaacaacggt taaaaattta taagttaaag cttattatag attttttgag gaagtttata
    47341 ataaaatagt taatgtttaa tttgaaaaga attaataaaa agctgtcttg atatagaaag
    47401 ttatttctaa taaaggacag tttttttata atactacaac atttgaaagt gttttaaagt
    47461 ttaatattta tttaaattat tataggttag aatggagatt tcaattatga gaatgtatga
    47521 tattataaaa aaaaagagag ataacctaga gcttagtaaa aaagagataa actactttat
    47581 agaaaactat tcaaagggag atatacctga ctatcaagct tctgcactat taatgtcgat
    47641 ttatttaaat aagatgaata aacaggaaac tgtacatctt acagaagcaa tgatgaactc
    47701 tggagacatg ataaacctat ctgagataga tggaataaag gtagataagc atagtactgg
    47761 tggtgttgga gataagacta ccatagctct tatacctttg gttgcttcat gtggagcacc
    47821 agttgcaaag atgtcaggta gaggtcttgg tcatactggt ggtactattg ataaattgga
    47881 gtctatacca ggtttttcga cagaaatgga gataagtaaa tttattgatt ctgtaaataa
    47941 gtctaaaata gcagtttgtg gtcaaagtgc aaaggtagca attgcagata agaaaatata
    48001 tgctcttaga gatgttacag caactgttga taatatatca ttgatatcat caagtgtaat
    48061 gtctaaaaag ttagcttcag gagctgatgc aattgtgcta gatgtaaaaa ctggtgatgg
    48121 agcatttatg aagacattag aagagtcttt tgagttagct aaatctatgg ttgatatagg
    48181 tactggtatg aatagagata caatagggat tgtaacagat atggatgaac ctttaggttt
    48241 tgcagtggga aactctttag aagttataga ggcaattgag acattaaaag gaaatggacc
    48301 taaagacctt gttgagctat gcgaagtatt aggagcatat atgttggttt tatcaaaggt
    48361 agcacatgat tttgaagatg gtgttgaaaa aataagagaa tcaataaaat ctggtactgc
    48421 tatacaaaaa ctcaaagagt ttatagagaa tcaaggtggt aataaagatg tagtagatga
    48481 ttattcttta ttcaaaaaat caaagtataa atatccagtt aaatcaacta aatcaggtta
    48541 tataagcaaa attaaagctg aagatgttgg tgtatcggct atgattcttg gagcaggtag
    48601 agaaactaaa gatgatattt tagatttatc agcagggata attttagaaa agaaagtagg
    48661 agattatgta aatgaaggtg aagttttagc ttacatgtat tataatgatg aacaaaagct
    48721 ttcattggca aagggaagat ttattgattc ctactctatt gtagattcaa aaatagaaaa
    48781 aaataaatta atatatggta ttgtaactaa agaagagatt aaaaagtttt agaaaatttt
    48841 agaaaagtga ttataaaagg cctaacatgt caaaaataat gttaggtttt ttattttttg
    48901 ataaactata aattatacca aaaatatata ttttaaaaca ttttttacaa tataaataag
    48961 tttattctac aagatgattg aaaattaaag tatctagttt tataatagaa aatggataaa
    49021 tatacataag gagtagatta tattatgaac tttaaagaaa atagattgaa aatagcagta
    49081 ttaataattg ttattttgat tatcttagca ggtatatttg tgtttataca aataggtcct
    49141 tatgataaaa ataacaaaaa agatgtaatt atcgacgttc caagtggagc atctgttgga
    49201 aaaatatctg acatattata tgaaaataaa ttaataaaga atgaactatt atttaaactt
    49261 ttggtaaagg ttagcaataa agctccgtct ataaaatcag gaacatactt acttaatcaa
    49321 tcgtattcaa acaatgatat aataagtctt ttagtttcag gtaaaatata tcaggatggt
    49381 ataaaagtga ctattccaga gggagctaca tctaaagaaa ttatagcaat gttagtaagt
    49441 aaaaatttag gagataaagc tacttttgaa aatttaataa aaaaacctca agaattttat
    49501 gataaatttt catatttgaa ggaagatgga ataacctctt tagaagggtt cttgtatcca
    49561 gaaacttatt attttaattc aaagaaacaa tcagaagaag atattttaag cgaaatgtta
    49621 aaagtatttg acagtaaata cacggacaag tttaaaaaga aacaaaaaga attaaatatg
    49681 actcttcaag aagtaatgga aatggcatca ataattgaga aagaagctgt tcttgacaaa
    49741 gacagaccta taatagcaag tgtgttttat aatagactta aggtaggtat gcctcttcaa
    49801 tcagatgcta caatacaata tatatttgaa gagagaaaga aaattgtaac atatgatgat
    49861 ttaaaaattg attcaccata taatagttat aaaaataagg gattacctcc tacaccaata
    49921 tcaaatcctg gtataaaatc tattgaagca gcattatatc cagagaaaac agactattta
    49981 tattttgtag ctaagattga tggtggaaat aattatagta caaactatca agaccattta
    50041 aaatacgtta aagaatataa agaagcaagg gataaacaat caaaagatac aaaagcaaca
    50101 aataaagaaa atacaaaaaa ataaaccatg attataaaaa gtctaacagg gtaagaataa
    50161 tgttgggctt ttttattgaa aattgttatt cttagattta taataaagaa agtgtaaata
    50221 tatataacaa tatgaattta aaaaataata aattaatata aaacttggat atttattatt
    50281 ttagggagtg tttttatgag taatatagtt aatgatttgg ttgaagatta tataagagtg
    50341 acattaaaag aaaaagaagg atttttaaaa gatttagaaa tttatgcaga ggaaaatagt
    50401 gttcctataa tacataaaga agtttcagat ttattaaaag tcttgttaaa ggttcaaaaa
    50461 ccaaagagaa ttttagaagt aggatgtgca attggttatt cttctatttt ttttgcaact
    50521 attataggaa aagatgcaga tataataact gttgagagaa atgaaaaaat gatagaaaaa
    50581 gcaaaagaaa acataaaatt agctggattt gacaataaca taactatact agaaggagat
    50641 gcagaagaaa aacttagtca agtccaaggt gaatttgacc ttatatttat agatgcagcc
    50701 aaaggtcaat ataagctatt ttttgattta gttatagata aattaaaaga tgggggactt
    50761 ttagtatctg acaatatttt gtataaaggt atggttgctc atgatgattt tgttgtaaga
    50821 agaaaaaaga ctatagttaa aagaatgaga aagtatttag attatatatg taattgtgac
    50881 tatttaagca catcattggt accaataggt gatggtgtag cactaagtta taaagaaagt
    50941 aaaagaggtg atgtagatgg aattaaataa agtagaactt ttagctccag caggtgattt
    51001 ggagagattg aaaatagcta taacatatgg agcagatgcc gtttatatag gtggagaaat
    51061 ttttggaatg aggtctgcag cgaagaattt tagtaaggaa gatatggcag agggagtagc
    51121 ttttgcacat gaaagaggca agaaagtatt cgtaacagta aatataatac ctcataatga
    51181 agattttcta caattagaag actatttgtt ggaactagaa gagataggaa tagatgcagt
    51241 tataatagct gacccaggag tgcttagtgt cattaaaaaa gtaattccga atatggaaat
    51301 acatttaagt acacaagcaa atacaaccaa ttatctaagt gcaaacttct ggtacgagca
    51361 tggaataaaa agagttgtag tggcaagaga attatctttt gatgaaatat atgagataag
    51421 agcaaaaaca cctttagata tggatataga agcgtttatg catggtgcta tgtgtatttc
    51481 ttattctggc agatgtttaa taagcaacta tatgactgga agagatgcaa acaaaggttc
    51541 ttgtgctcaa tcttgtagat ggcaatatca tttggtggaa gaaaaaagac ctggtgagta
    51601 ttttccaata tacgaagatg aaaggggtac attcttcttt aactcaaaag atttatgtat
    51661 gatagagtat ataccagagt taatcaaatc tggtataaca agtttaaaaa tagaaggtag
    51721 aatgaagact gcatactatg tagctactgt tgtaagagca tacagaatgg caatagatga
    51781 attttacaga gacccagaaa actggaagtt taatccaatg tggatggaag aactaaaaaa
    51841 aggtagtcat agacatttta catcaggttt ttacttgaat aaaccaacaa cagaagacca
    51901 aaattatcaa tcagcatcat atgttagaaa ctatgatttt ataggtatag ttagagaaac
    51961 agaagatgaa gatggtctta ttgttgttga gcaaagaaat aaaatgtgtg ttggtgatga
    52021 aatagaagtt atgggaccat ataaagaaac tatgtttact aaaattgaag aaatgtataa
    52081 tgaagaagga gaagctatag aatcagctcc tcatcctaga caaatagtga agttaaagct
    52141 atcagtaaaa gttggtaaag attatatgct tagaaaagtt atagaagaaa aggttgaaga
    52201 ataataaaaa attgataaaa atagtattag ccccttagaa attgtcaata atgaaattat
    52261 ctaaggggtg atttcatgtc aaaaaagaag actccatttt tgaaaaaagt gggaaagaga
    52321 agctggtgta tatttactat aattttaata atttatagtg ttttaattta taggctagtt
    52381 gatatacaag tattaaaagg tgataaatac aaacaaagtg ttgaatcaca aagcgttgag
    52441 aaagtcgagt taaatagtgg aagaggaata atctatgata gaaacaacaa gaagcttaca
    52501 gatacaagta aatctcaggt attaattgtt gaaaaagaaa aattaaataa taactataaa
    52561 attttggagc ttattaagaa agctactaag atgaatgatt tagatatata taaagctgta
    52621 caagagcaat tgacacgccc tataatacag attcagacta aaaatatcga tatcgataag
    52681 agtatgaaaa aagaacttga gaaaaatgga ataatggtag aagaaaaaac tatgaggtac
    52741 gcaaaagatg gtttattatc acatacaata ggatatataa aagaagatga taaatcaggt
    52801 caatcaggaa ttgaaaagag tatggatagt gtacttagaa attcaaatga aaagtatata
    52861 agtgctttta aagctggaga tgcaggaaat gaaaaatctc taaacatact aaagggaagt
    52921 gtaaaaactg ttgataataa ggataaagat agacatctaa aaactactat tgattataac
    52981 attcaaaaaa aattagaaca aatactaaat aaagaagaaa atcctacagc agcaataatc
    53041 tcggaagcat ctacaggtga aattcttgca atgtgctcta gacctaattt tgaccaaaat
    53101 gacatatcaa aaagtttaaa aggaaaaaat ggagaatttg aaaatagagt tataaaagcg
    53161 acatatccac cagggtctgt atttaagatg gtagttttat tttctgcact tgaaaatggt
    53221 gttatagatg agaactatac ttacaactgt acaggtaaga ctaaagttgg gaatactaat
    53281 gaaatactgc gatgtaataa aagagatggt catggtttcc aaaacttaag acaagctttt
    53341 tctaattcat gcaatcctgc atttttagat atagctatga aacttggaaa agaaaagata
    53401 ctcaaatctg ctgaaaaatt acatttattt gaaaaggttg atattggtct tgatgaagaa
    53461 aaaattagag aagctccaaa aaacatatca ataagaaatt tagctatagg tcaagaaaat
    53521 atagaattta caccacttca aataaatcaa atgactcaaa taatagctaa taatggaaca
    53581 ttcaaaccac tatacttata taaaagtctt gtggacaata atatgaatac aataaaaact
    53641 tataagtctt caaaaaaaga agaacttatt tctccttatg tgtgtacaca agttaaagaa
    53701 tatatgaagt ctgtatctag gactggtaca gctaaagacc taaaagatat tgaaggtggt
    53761 tgtggagtta aaactggtac agctcaaagt agtttaaata aaaaagctat agaccacgga
    53821 tggataacag gattttatcc tgaagaaaga cctaagtatg taataactgt gttagtagaa
    53881 ggaactcaaa aaggaaataa atctgcaaca cctatattta aggaaatatg tgaaagtata
    53941 aaataaatta tgtactataa aatttaagga tatatcgtaa ctttgatttt agatagaaaa
    54001 ttttgtctaa tgatgtacga tatatccttt taaaatatag tattaacttt cttgaacaag
    54061 ctctttttct agttttttaa gagctttttt ttcgattcta gatacatatg accttgatat
    54121 atcgagtttt tgtgcaattt ctctttgtgt cttatagcca taaggagtta acccatatct
    54181 taactggaca atttctcttt ctctaggagt aagaattttg tttagttgtt cgtaaagttt
    54241 tccaacttga acttttagtt caacttcatc aagaacatag ttagcctctg taccaagtat
    54301 atctaaaaga cttatttcat tacacactac tctttatatg ttattggtat tactggtctt
    54361 catgtaattt caaatggacc attaattcaa aagaatctag tttttgttct ttagacttat
    54421 aataaactgc tttatcaatt acagatttta ggagtttatt ttttccaagg gaatctgaat
    54481 catggtaaag tgataacaca gttttaatat cttctattat tttagaattg tcttttttaa
    54541 cagtactcaa tctatttttt actgtcttaa ttgaatcttt aatttcttca gttcttgaac
    54601 ttatattgtt ggacctttca ataaatgtat cttcatcata tattcctcgt tctagtaaat
    54661 catataactt atttttttgc ttgcctaatt cttttaattc agtttctaag ccctttaaga
    54721 ctcgtttttg aaattctata ttactatctt tattattttt atctaagtct atatcagatg
    54781 cttttaattg atattttttt acagtgtctt ccaaagattt taaaatggca gcttcaataa
    54841 attcaaatcg agaactttta ttgcaaccag gatgataaca aattatatag tcgtggtctg
    54901 catatggtct atatactaat ggtctaccac aatttttgca aatcactatt cctgcaagtg
    54961 gattttttat ttgattacca tagctgacat gaccattttt tttgagtata ttttgagctt
    55021 ttttccatgt tatttcactt attaaaggtt catgctttcc ttttgcctca atccattcat
    55081 ctttaggtct tagtttaact gtttttattt tatttggatt tttagatttt ttgtagtcct
    55141 ttttttgcca ctgaatgtag ccacaatatg ccttattttt tattatattt gtaattgcag
    55201 aattgtacca attagcacct gtagcagttt taatgcctaa gttatttagg tattttgata
    55261 ttttactaca acccatatct tcattaatat ataagtcaaa gatagtccta actacatcag
    55321 catctttatt tggtacaagt attctttctt ttctaccagt tgttacagca tcatatccaa
    55381 atggagcaga ggttcctatg aaattccctt cttcaactga ttttattcta cctctttgca
    55441 tccttcttga tataagtttt aattcttttc ttgccataaa tgcttcaaat tctgaatact
    55501 cctcatcaaa ttcatctgtt aggtcgtatg tttttctagg tgtgatgatt ttggtcttac
    55561 tttctttgaa tgtttctaat atgatacctt ggtctttcat gtctccacga ccaagtctgt
    55621 ctaagtccat tactaagact gcatcatatt tattttcttc aacttctttt agaagttcaa
    55681 gcattttggg tctatgtatt atactttcac cagaaactaa ttcttctttt atctcaatta
    55741 catctaagtt ttgttctttt gctagtttta gtagggttga tttatgtcta cttaaagttt
    55801 caaattcgcc ttgtttttct gcctcttcat cagcacggga ttttcttaag tatatggcaa
    55861 cactcataat aagtaatcac actccttatg cactattata tagtaaatca atagagttta
    55921 aaatatagaa aataagcatg tcaaaagtta atttaacttt tgacatactt attttctata
    55981 atattaatgc cttataatcc tttaatatca tttagattag tttttgcata ttcattatcc
    56041 gggttaagtt caagcgcctt attgtaagat tctctagctt tattaaattc tgatttactc
    56101 ataaaaataa cacctcttaa attgtaagca tcactagcgt ctattttata agcttcatta
    56161 tttttatcta gatattctaa gtatttatca attgacttta aagcctcatc atatcgttgt
    56221 aactctatat aaatatatga ttgtgtataa tatgcaagtg gatttttatc atcaatttct
    56281 atagcttttt tgatatcaat agcagcttta ttataatcct tatcttcatc tttttctgtt
    56341 tctgacctag cgattttgac ataattagag ctaagagctc ttttaaagtc tttatgccat
    56401 aataatttat attcattatc ttcgattata aaatatctat caaaatttgc agtatcttct
    56461 ttattttcag tgatatactt tatagtatca gtttctttta ttttaacaag atttgtgtac
    56521 tctttattag aatatttgta tttctctact ttattttctt caccaagttt ataatctttt
    56581 agggtttgaa gtttaaataa agtatcttga tattcttcaa atttttctag actgacactt
    56641 tccttactgt ctttacttaa taaattatat gctacttcgt agtctttgat ttctatacta
    56701 tcaaggtact ttttagctac ttctgtagca ctttcttttg gagcacatcc aaccattgat
    56761 atcataatta aaaatgatac taatattgct gttatttttt tactgattat cataatataa
    56821 tcccccttat attgtctctc attaatttta ttttatcacc atattctacc aaagtcatta
    56881 atttaagagt gatatttttc attaatatta tattaagtaa taataacctt ttaaatttat
    56941 ggtaaaattt tcctgaaaag tattatataa tttaggtgaa aatatttaag agaaggagat
    57001 attatgaaaa ttaaaaaatt attaacaata gggttatctt tatctatatt tattgctagt
    57061 tgccctatat ctgtaaatgc tcttgataaa attgaatcaa ttaaaggtgc agataaatac
    57121 gaaactgctg gtataattgc agacaaacaa aactatacta cagctatatt aattaatgca
    57181 gacagtacaa tggctgatgg attaagtgca agtggacttg caggagctat aaacgctcca
    57241 attcttttga ctaagaaaaa taacatacca aatgctacac ttaaaagatt agaaaaggca
    57301 aaaaaagtat atataattgg aggagaaaac tctattgata aatatactga aactgttcta
    57361 aaaggcaaag gaatagagat taaaagactt caaggtagtg atagaattaa gacaagctat
    57421 aatgtcgcaa aagaaataaa ctcaattaac aaagtaaata aagtcatatt gactaatgca
    57481 tttaaaggag agcctgatgc aatgagtgct gcacctgtag cagttagaga taaagcagct
    57541 atagtattaa ctgatggaaa aagtgttcca tttaatacaa ctggtgtaga aagttatgta
    57601 ataggtggta cttcatcaat gaatgacaaa atagttaagg atactaattc aactagatta
    57661 ggtgggacag atagatatga tactaacaaa aagataataa ataaatttta tagtggagta
    57721 aaagaatttt atatagcaag tggtactgat ttagtgtatg ctcttgttgg ttctactgtt
    57781 gctaaaaata atgctattgt gttagttgat aatgatagta ataaaagtgt attgaaaagt
    57841 acaactaaat taactgctat aggaaatttg agtgatagta tattacagca gtgtttaaat
    57901 gtaacaaaaa atataggcga ttctaatact ggaagagaat atacagtaga agatgctgct
    57961 aatgcagtga aaaaactatt aggtgtgaag aaattttatt acgaacctaa ttattcagaa
    58021 gatgaagatt taatatttct actagataaa aattttgatt tttcaggaaa agagtactat
    58081 gtatttgctt acattaaaca gggtgcagag ggtgatgtta gatactgtgt agctaaaaat
    58141 gatatgagca aagtttatac atatgatgtt aatggtaata tgaaagtatg taatccagat
    58201 gattataaaa ataaaggaag tataacagag gaacaagcaa aacagatagc attaaaagaa
    58261 tgtgctaaga gacataaagt cagtatcaat aacttagtag ttgatatgat agattttaga
    58321 gatgatatat atagtggtaa tgtctatgct gttaaaatca attcaaaaaa tagtaatggt
    58381 acttttggtt ggttttttat agatatagaa actgggaaag tttataatta aaattagaac
    58441 ttttaaacaa gtgtttttaa ttatataatt taccataata tgttataata tttttagcaa
    58501 gaagatttaa cttacaatct atagagtgga gttcatgtac aaaagattat cctcccaacg
    58561 attcgaaggg aggtgagttc atggataact ttttgtttaa tgttttagct agtttaacag
    58621 ctagtgtggt agtttactta attagtaaac tattcaaaaa agcaaaaagc cactctcgca
    58681 caaagagtga cttacgagtt gaatttaaat ttatatttaa atccaaaaaa taaactctgt
    58741 ttatgatgaa ctccactcta acgcaaaata gattgtagtt cttcttgctt ttattatacc
    58801 acaaaacatt ataaataaaa ctgttgttta taaaaaaatg caaagtaaaa ttttttataa
    58861 acaatgataa aaattttatt atagagctat taacttatta aatatctgta aatgagcaga
    58921 tatttcataa gttaatattt attgtttaca taaaatattt ggtaaaaata agtaagtttt
    58981 atatgttata atagttgtag caagaataat aatctaaagt ggcaagcatg tcagctggtg
    59041 ttatatcagt ttatatttat gataaaataa aaatctccca aacgccaata agaatgattt
    59101 aaaaaaataa tattttcact taaaaataat cactctttat aggagtaaat tattttcttg
    59161 attttattat actacaaatt ggtacagata tttaaaaata atatattcat aatattataa
    59221 atagttttgc agtgagcgat atttttgata aaatagggct taacagttga aacataaggc
    59281 attgagggta tatgataaat attatcattt gcactactcg tggttcactg caaatttgag
    59341 agaattgtat agatgtaagt cttggaaatg ctcaatttat tttggggttt tatattaact
    59401 aagtggtatg taaagtcgta aattacgacc gtacctttcg taaaataaca atcgttttat
    59461 attaactaag tggtatgtaa agctttttga gtatgagtac aagcatagga aaattaatag
    59521 ttttatatta actaagtggt atgtaaagaa caaaaaagat acatctgtaa ctaattttta
    59581 tactgtttta tattaattaa gtggtatgta aagacagata taatatcaaa ataaggttag
    59641 gacttttaaa agcatagcca tatcagtaaa aatttaatta tcatttaaaa aataaatttt
    59701 tatttaaaga atacacataa atatttccag actcaactct cttaaactat tttcatatct
    59761 tttagtagat acctttgttg ttttttaatc tttagcaact tgttcttgag tgaacctctt
    59821 attttttcta agttatttca agcttttcaa aaaattctct atttatattc atcacacata
    59881 taatacaatt aattcagctt taattgccaa tatttagttt ttctgtatct gataaaccaa
    59941 gaatataatc agtatataaa ccaaaaattt tagcaaatat tattaaccca tcgtctcttg
    60001 ttggtctttc accagactct attctattca taacacttgt atttatattt gttttttcaa
    60061 gcaattcttt ttgagaacta ttcatatttt ccctaatata tttaatcctt tgatttacat
    60121 ccatacataa ttatctcgaa aagtttctgt tttggaaata ttgttattga aatatagcaa
    60181 ttaatgtgaa tatatgaaaa agatacacta tgattttata aaaaagaaga catacataaa
    60241 atttatactt acaaagtgtt gcaaatgagt taggttttaa aaatgcatca atttatttta
    60301 agtacaggaa aggggaatat tcaattagag ttgatatgtt acctataaac tatctaagat
    60361 acttgattat gatatagaaa atttttttac aaattaaatt tctaagatag aaatttaatt
    60421 aagaggatga tgaggatgaa taatttacaa gtaatataaa gaaataataa gagaatttta
    60481 aatgcatagc aactagcaga tatatatgaa aatgatgtaa acaatatata aactaacttc
    60541 aatagaaata aagagaatgt aaattatgtt ttattacaat tcgagtattt gaaagaattt
    60601 aagaactaat ctattaatag tcagttgatt tcaaaacatt ctagtcaatt atacctttgg
    60661 atcgaaagat gtgcaaatag acactgcaaa atattagata ttgataaaac ttgggaacaa
    60721 tttgacaatt tagagaaaat atatttcaag attaagcgag agaaactaag ctgtatagaa
    60781 gatgtattaa tagaaaattt aagatttaag gatacaaatt aataaagcaa taatatggca
    60841 ttagatgtaa aaatagaagt taaaataata aaagatgttg ttatattaaa tataacacat
    60901 tggaaaaaag aaacaagtct attaacagtt aaaatagcta gaagtcaagg tgatttaagt
    60961 tatgtaaaat attttgctaa tgagagctaa gagcttctta acaagagttt tggtgtgtct
    61021 cttgaaacaa ggttaacaat taataaaaga cgaatggcag aagaaggcgt atgtaagttt
    61081 aaaagagata agttaaacaa actagatact atgtaagagg ataagaaact tatagaagaa
    61141 tatgttactt ttgtaaagga tttagcttta aaatatagaa tataaagtga ttttaagtaa
    61201 aaattgtaac aattaaataa gtataagaca agtttatgag ttcatatttt ataacaaggg
    61261 gtgatgttat gaaaatacta caacaattta aaccatacaa atatgataaa gaaaaaaata
    61321 tagaatatga tgaaaaaatt atagctaatc aaaacacaga agtttatata gtatatccac
    61381 gtatatctga tgaagaaaat aggagaagat gggaagagtt caatgaagta gctaaagaag
    61441 tagctattaa tatagctatg aagaaagttg agacagtaga aagttaagag aatagagaag
    61501 ttgctaatac ttagcacttt ctcatagaag aaaattggac aactattttt aaataattag
    61561 gaataaaaaa gttaaatcat atttgaaaaa aggcgataaa attgttgagt attgttatta
    61621 gtaataagaa tatattaact atgtattaaa agaaattata agtttttagt aaatatagta
    61681 tttaaggata ttaaaactgg taaaaaatat catttagact attaaataaa aaagtgctgg
    61741 tcaaagtagc aacacaataa ataaaaaaac ttatagaaat tataagataa aaagatgaga
    61801 tttgaaatgg tcgaagttaa tataaaaatt aaaggtgaag taagtgaaat aattaactca
    61861 attagaggta tatttaatag ctttgatgaa gaattaatat tagatataaa atcagagtaa
    61921 gaatataaag gtagatacgt agaaagttat aagtaattca agacttaaat taatttctag
    61981 tttttttaat gcaaaaattt aataaatttc aaaatataat aattctgaca tctaaaaaga
    62041 gctattaaaa atgaataata ttgactatta agatatgaat agtgtacata tattaaatta
    62101 agatatcaag taggttaatt ttatataatg atggggctca aattgaagag gcgatataaa
    62161 aatgtattaa tatatataaa tataagaact ataatacata ttaactgtaa aattaagttt
    62221 atagagttat atttaacatt taatgaactg aaaaataaat tagcaaataa tgaaaaaaca
    62281 acagtaataa atgagaataa caaagtattt tcaggattag tggatttttc taattgagct
    62341 ttaatcctag tttgttcaat ttcgatattt ctaatattat caatttattt gtaataatta
    62401 tcaatttttg cgaaatattt ttgataattt atagaacttt atatattttc cttgaagtat
    62461 ttttcgccct tattgtttgg tttccaattt taaaactcat aaactcccaa taaattcatg
    62521 tataatatac cttaatttat aatatattta agagtttatt tcagtaattt ttcgaacgat
    62581 tttgatatat ttcatttata gttttcaaaa acttatacaa aaaagagtgt ttcatctttc
    62641 taaacactct ttttataaga atttgaataa taatttttta aaaattgata atatggttat
    62701 ttctttttca agtttatagt ttcctcattt ccattccaag atataattag cttgtaacta
    62761 gattctctat ttgaataaag acctggtctt tgtattttta ttgctttttc ttgacctgct
    62821 tctaaaattt cattttttac aactacaact gcatcttcac catctataaa ttgataggaa
    62881 taattaaatt tttgttcaac tacatcttta ctatttttat attttatttc taattttgag
    62941 tgtccttcag tagttacaaa tttagctttc caattttctg attctccaat aaagacattt
    63001 ttatcatttt ttatattaag tatagctatc acagagatta ttaaaactat aagaatacct
    63061 ataaaaaatt ttttcctcat atcaatccct cctttgaata attataacat gagaatagtt
    63121 attcaatact aattttaaaa atattgtgga tttatattct attacaatag aatgtctgtt
    63181 ataacaaata aaaagtaagg attaatatga attataggtt aaaaaaatct gtaacaacta
    63241 ttttatcttt actcataatg acaatactat taccaacaaa tgcaagtgct agtaaggtag
    63301 aagattcatc tatgaaagta atgtattcag agaaacaagt aacagacatg gacaaattat
    63361 acgatatggc taaaaatgga attactgatg ttaaaagttc caatgaaaaa ggtataatca
    63421 aaaatgcaag aacaggagag tccatatacg ttgattctat atcaacaact caattattag
    63481 aagttaaaca atctaaaaat ttaacatcaa agatatattc taagacagtt tttttgatac
    63541 taatacaata aagcctctta gaaaagcaag tatatcagaa gatggatggg ataaaactgg
    63601 tggtgtaaaa gcatctggta cagttacata tacaatatca aaaggagatg gagcatttca
    63661 atatatttcc gttagtaaag tttctggaaa ctggtctatt gcagacggcc agattcgtgt
    63721 aacaaataga agggtgcata taaatcaaac tggatttatt tctaatgagc taggaattaa
    63781 gcagcaacaa aagaaagagc caacatcaaa ttctttctca tataatgcac cttctagttg
    63841 gaaaccagta ggtagagatt cagttcaata caattgtggg tttgaattaa ttgcaacatt
    63901 agtaagagga ggaagttctt gggcattcaa tttcattgtt caaccttaaa atattttaag
    63961 tgaattttat taaaatcaaa gatatacata tactttaaaa ttagagaatg taaattaatt
    64021 tgtgttttta aagataattc ctatgttata gtaacaa
//
LOCUS       DAMJVK010000008        61922 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.008, whole genome shotgun sequence.
ACCESSION   DAMJVK010000008 DAMJVK010000000
VERSION     DAMJVK010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 61922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61922
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.008"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            239..355
                     /locus_tag="PC414_000540"
     CDS             239..355
                     /locus_tag="PC414_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein"
                     /protein_id="HDF2462953.1"
                     /translation="MDDDINMVAIRPEDIRVEFNRDFKEIIDLKLRPYRYKN"
     gene            519..1586
                     /locus_tag="PC414_000541"
     CDS             519..1586
                     /locus_tag="PC414_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2462954.1"
                     /translation="MKFKKLVSLGILSTLLLSVITGCSNVSSGSNLKSNNKTKGELTV
                     YTAIEEDSIDTYLATFKEKYPDIKLNVLRLSVGGITARLLAEKDNPKADVLWGVAATS
                     LIILDDKEMLEPYAPKGFDKVNSAFKDDKKVPSWVGIDAWMTGITVNTKELKAKNISA
                     PSSYEDLIKPEYKDLISMPSPASSGTGYLTVSELIQIMGEEKAWQYMDKLHKNIGVYT
                     QSGSAPAILAASGEYPIGISFGNRGIKLKGEGYPVEVVFPKEGSGWEIESNALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNFAVTTIDTGNPVPEGFPKNPLEQMIDNDLKSAAR
                     NREAILNKWIEKYDGKTEKES"
     gene            1778..2308
                     /gene="rnmV"
                     /locus_tag="PC414_000542"
     CDS             1778..2308
                     /gene="rnmV"
                     /locus_tag="PC414_000542"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HDF2462955.1"
                     /translation="MIKEIIVVEGRDDVTAVKRAIDAELITTGGFGFPKGVMERIKAA
                     NERRGVIIFTDPDFAGEKIRKKIASEVPGCKHAFLPREEAKKDGDIGIENATPQSIIA
                     ALNKVRTENTEKRDEFKQVDLIRNGLIGNEEASLRRDALGKILGIGYGNAKQFLNRLN
                     NYGVEREEFIDALKQI"
     gene            2479..3348
                     /gene="rsmA"
                     /locus_tag="PC414_000543"
     CDS             2479..3348
                     /gene="rsmA"
                     /locus_tag="PC414_000543"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HDF2462956.1"
                     /translation="MDRLSSHNATKEVVQKYNFKFSKSLGQNFLIDSNIIDKILSGAR
                     ITRGDNIIEVGPGIGTLTREMGKIAEKVVAIEIDRNLIPILKDTLSDLDNTEVVNQDI
                     LKVDIQELVKDKLNGGPVKLVANLPYYITTPIVMKFLEEDIPVTDIVVMVQKEVADRM
                     NAIPGTKDYGALSIAVQYYCDTEIVAKAPRHMFIPQPNVDSTVIGLHVRDKRKYDVHN
                     EDIFFKTVKASFGQRRKTLLNSLGGLGFLNKDEIREVLKEANIDEKRRGETLSIEEFS
                     VLSNIINTKVSSK"
     gene            3414..4676
                     /locus_tag="PC414_000544"
     CDS             3414..4676
                     /locus_tag="PC414_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2462957.1"
                     /translation="MGSKRKFKRDYIILEAKDINFRYKERVLPKAFAKVEVNDEKAII
                     ALYVENLKYVKDGYKAVAIKDDHGLIDLGNIILNEQGRGEFVLDLEDYDDEIKGIALL
                     YERNIPLIGFKGNKLENYEDILFQEVEEEEYEEYEELEEEDEEIEENEESDRDEDSEE
                     EYIIEEYDEDEYEDYWEDDEDIEEGELKARTEVEDSDDEEIEEFNNYKEIENYNRVKV
                     QEEYIDIEYDEEEEEEEVFEVNREEDRFVSSKPTKSKKVQKDQNNIEKIKRANGVSQG
                     ESGNKQTSTLLMPRQIKKGLKLFKEVKPFVTDYIDKTRWWKIEINPTTLCGYTMPYLG
                     YLNVLNYTMYSDAVLHSYKYRHYLFGVQYDEYNKRKHYIYAIPGNRNEQPDNGSTGFL
                     RYQACDNRTNSLGYWLCFIDSRQRNIVR"
     gene            4827..5942
                     /locus_tag="PC414_000545"
     CDS             4827..5942
                     /locus_tag="PC414_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDF2462958.1"
                     /translation="MINEQRILNEFLELVQIDSLSLKEGNVAKVLVKKLEEIGCSVVI
                     DNAGEKANGETGNIIATLKGNKEGKKILFSSHMDTVTPGIGVKPIVDEANGIIKSDGT
                     TILGSDDKAGIAAILEGLRYINENNIEHTDIQVVFSICEECGLVGAKNLDYGKIDSEY
                     AFILDSGGSPGEIIVKAPAQDVINVKILGKTAHAGLEPEAGISAIMVAARAIENMNLL
                     RIDEETTANIGIINGGTATNIVTGEVNIVAEARSLKENKLDVQTKHMVETFEKAAKDF
                     GAQIEIDVNRAYTPIDVSEDSEIIKLAKKAFSNLGIEGHTESTGGGSDTNILSKNGIE
                     AITLGIGMKNAHTLSEHIAIKDLYDSAKMVVEIIKEA"
     gene            6128..7024
                     /locus_tag="PC414_000546"
     CDS             6128..7024
                     /locus_tag="PC414_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDF2462959.1"
                     /translation="METRYEEANKITIQSILWNIVLTIIKVIAGVIGNSSAMIADGLH
                     SASDIISSIGVLIGNYVSSRPGDREHNYGHEKAETLVSFVLSILLIFVSITIGIEAIK
                     SLFNLDALSVPSILPLVVSVISILIKEYQYRITIKVAKKINSPALKADAWHHRSDALS
                     SVAAFIGIGGSILGFKPLDPIASVVVAIFVAKVGISILISSVNELMDVSVDEEEIKEL
                     KFIVADTEGVKNLGDIKTRKHGAMAYVDLTICVDENLTVKQGHDIATKLEKHIIKHME
                     FVKGITVHVEPCTNCQGNKCNN"
     gene            7094..7891
                     /locus_tag="PC414_000547"
     CDS             7094..7891
                     /locus_tag="PC414_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2462960.1"
                     /translation="MSKKRKLKKKAAILIGVVVFFIIYFISFRIGINMEKDKSEVPKK
                     VEKEEEDNRSLRTQIKEKKKVYISDKNIENIKIEEDMWDEIKLFFSDFKKVRNTTSSY
                     EAVYEGYSDDGVKFSTDLNFFRVYTVNKEEYYKVPVSSKDAFKKLLDESIYTSFDLLS
                     HYKDWKEVTITHGKEIKKVHRWKFDDLAHKMISKRVVGKVQPEKSKERSDYNFTINIK
                     GDNFDVKVDTMGKDYVKVTSKKNVSYYEVHTSLFDYLKNEIFEIGKK"
     gene            complement(7946..9298)
                     /locus_tag="PC414_000548"
     CDS             complement(7946..9298)
                     /locus_tag="PC414_000548"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HDF2462961.1"
                     /translation="MNIHFIGIGGISMSALAEICINKGYQVSGSDSNESYLLDKLRDQ
                     GATIYIGQKKDNISDDVNMVVYTAAVHPDNEELVAAKEKNKLVMNRATFLGQIMREYK
                     NSIAVSGTHGKTSTTSMLSTIFEYADLDPTILVGGNLSMIGGNVKIGNSNHFITEACE
                     YVDSFLNFNPKISIVLNVEEDHLDYFSGIDEIKASFNKFGKLLPPEGYFIINGDDENI
                     DDILYDVKATIIKYGRDSDNDAVIKDIHFDNSGHGIFRIEYEGRDLGEFELSVYGLHN
                     IYNASSAIMAALVSGIDLETIRKNIKIYKGVGRRFETKGYYKNALVVDDYAHHPTELK
                     ATLAAAKKLKKSTLWCIFQPHTYTRTKSLLGEFSEAFYAADKVIITDIYAAREKDPGD
                     IHSKDLVEKLYQNNVDAIYIKEFEDIVKYLRENVKDNDLVITAGAGPIYKVADLLVEK
                     "
     gene            9517..10395
                     /gene="purR"
                     /locus_tag="PC414_000549"
     CDS             9517..10395
                     /gene="purR"
                     /locus_tag="PC414_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HDF2462962.1"
                     /translation="MKFKRTERIGAIVKILSDNPNKIYTLSYFTNQFNAAKSTISEDL
                     LVVKNVFEKLHLGKVITISGAAGGVKYIPKTSIAENAEFLMELCERICDKSRILSGGF
                     LYLIDLIYDPTIAAKIGKIFASNIEYVDADYVVTMETKGIPMALMTAKAMNLPLVIIR
                     KDIKVSEGPTLSMTYVSGNSSKVESMSLPRKALKPDSKVIIIDDFMRGGGTIKGMVDL
                     MNEFGAEVIGTGVFISTTNPSEKMVKDYISLIQLDVNGDKIVVEPNLKTFKDEYRNED
                     LDEEEDSEFEFEIDED"
     gene            10551..10829
                     /gene="spoVG"
                     /locus_tag="PC414_000550"
     CDS             10551..10829
                     /gene="spoVG"
                     /locus_tag="PC414_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HDF2462963.1"
                     /translation="MKITDVRVRKLTEEGKMKCIVSITFDNLFVVHDIKVIEGHNGLF
                     IAMPSRKVGEGNFRDIAHPINAEMRQVLEDAVLQAYHEALVQWEVAAE"
     gene            11042..12421
                     /gene="glmU"
                     /locus_tag="PC414_000551"
     CDS             11042..12421
                     /gene="glmU"
                     /locus_tag="PC414_000551"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HDF2462964.1"
                     /translation="MNFKAIILAAGKGTRMKSKYPKVIHKVCGKEMVNHIIDVSKKSG
                     VKDTVVILGHEADVVKEKLAEEIIIAMQTEQLGTGHAVKMAKEYINDEDTIVVLCGDT
                     PLIKEETLKRLFEYHIENKYHATVLTTRVGNPTGYGRIIRDKKGDLLKIVEQKDANSE
                     EKMISEINSGIYCFNGKSLREALDLLNNNNSQGEYYLTDTAKIMRDKGLKVGAFAGST
                     IEELMGVNSRVELSKAEEIMRRRINESHMVNGVTIIDTNSTYIESDVMIGNDTIIYPG
                     VMLQGKTRIGSDCIIGMNSSITNSEIGDGTEIKNSTIIDSKVGENSTVGPYAYLRPKS
                     DLGNNVKIGDFVEVKNAIIEDGSKASHLSYIGDAHVGKNVNIGCGVVFVNYDGKNKFK
                     SIVKDNAFIGSNSNLVAPVVVEEKGYIATGSTITHDVPDGALAIARERQVIKEGWVEK
                     KNQKDDQSK"
     gene            12516..13466
                     /locus_tag="PC414_000552"
     CDS             12516..13466
                     /locus_tag="PC414_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HDF2462965.1"
                     /translation="MNTSGSEIKIIAGNSSKELAQKIADYIGVSVLDCEVGTFSDGEI
                     CVNMNETVRGCDVFVVQSTNSPVNDNLMELLILIDALKRASAGRITAVIPYYGYARQD
                     RKAKARDPITAKLVANLITAAGADRVLTMDLHAAQIQGYFDIPLDHLLGGTILANYFN
                     EKKIEDLVVVSPDLGSVTRSRKFANTLNGEVPIAIIDKRRPKANVCEVMNLIGDVKGK
                     NVILLDDMIDTAGTIVNAANALKEFGAKDVYACCTHGVLSGPAIERIANSEISELIVL
                     DTIQLPEEKRIDKIKIKTVAPLFGDAIRMIFSNESVSKLF"
     regulatory      14304..14389
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            14551..15072
                     /locus_tag="PC414_000553"
     CDS             14551..15072
                     /locus_tag="PC414_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDF2462966.1"
                     /translation="MKLKKNKKGFTLVELLVVIAIIGILAVVAVPALFSNINKAKVAS
                     VESDYSSVKSAALSYYSDTNKIPVTPDGQTGLSVLETYMESLPDKADIGGKYKLIKVG
                     NKLVLQIGTNTEGVTLTEAQSAKLLSDIGENKIYTSATTNSLGNPLTSNTKIDNNVLY
                     IVLIDNTVMDTTK"
     gene            15223..16899
                     /locus_tag="PC414_000554"
     CDS             15223..16899
                     /locus_tag="PC414_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HDF2462967.1"
                     /translation="MAKKVRIGDKLVEKGYITEEQLKWALSEQKNSGKRLGEFLVQEG
                     LIDSNLLISVLKELLDIESIFLEGTEIDTLATKMVPENICKRYTVFPFKIDGNKICLA
                     MSDPQDREAVQDVRRMSGKDVEIFISSTEDINKAIGHAYAHSEINKAMTEYNKNRTGG
                     VRETVILEEDVNAAPIVRLVNNILENAVRMEASDIHIEQSENYMRVRFRIDGMLREYM
                     RMNSAPYKAVISRIKIMSDINISEKRIPQDGRIYLKVDNKPIDFRVSTMPTNRDEKIA
                     MRVLDKSNFMVSKEVLGIDEHGSKIYDELINTPYGLILVVGPTGSGKTTTLYSMLNQL
                     NTENRNLLTIEDPIEYELPGVNQSQINEKAGLTFASGLRAFMRQDPDIIMVGEIRDTE
                     TAEIAIRASLTGHLVLSTLHANTAVGAISRLLDMDVESFLITSSVLGVISQRLTRKIC
                     EHCKVSYEADIGEKKALGIDVNESVTIYRGKGCERCNNTGYKGRLGIFEMLEITPEIK
                     ELIDSSANQREILKMARKQGMVSLKEDIVKKVLNGKTTVEEMIRIILMTD"
     gene            16911..18119
                     /locus_tag="PC414_000555"
     CDS             16911..18119
                     /locus_tag="PC414_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HDF2462968.1"
                     /translation="MKSFKYTVIKEDGKKQTDVIEANDFNEARNLLRKRNLRVIEIKE
                     SKNKNIRLSSRKRKKDLGADQISHFCRQFAIIVSSGINSISGLETLARRSTNITLREE
                     INRIVAEIKVGSTIADSMLSSKSKFPKLLGAMVATGEATGKLDEVLKSMASFYASEHR
                     VKQKLRNAATYPLIVLISSFIMIFIFTTFMVPKMINSITTVGATLPLITRIVMGFGMF
                     MKKFWIIVLLVIILFIYQFKKYLKTSVGRAHKDRVINKIPVLGKGINCMVATRFSRAL
                     YLFVSTGYPLVQGLDYIIDSVNNTMAEKILASAKDGITRGEGLAENLERYTYFDSVLV
                     QMIAIGEQTGELENISRQMAEFYEYESEIYLNRMASMIEPVLIIAVGIIVAILVVSIF
                     MPMLSIYDAM"
     gene            18134..19846
                     /locus_tag="PC414_000556"
     CDS             18134..19846
                     /locus_tag="PC414_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HDF2462969.1"
                     /translation="MNKVYVSYYGDSISIVEGRYKHDKDKFYVKNFNVFSIEDIAQDF
                     SKEKYALLRYALENVKLKSRHVVFCLNTRDVILKPQKLPNMDKKDLDGFMKLEMDEMM
                     ALESDEYVFSYEVSSETPDNSDGDESSLNLIIGAIQKEEVNAIVEIMHEFDLVLDRID
                     TLSTAYLRMLKNLDYNDIMVTNISDNSTIINIYKKDTLFISDNIPIKITAKDMDMQLL
                     NVVEESKGLMNYYSSRNFGKVTDTILILGKRYSNKLIYETFSSAFSSYVSQGLTEIMD
                     ISDMITGDIDEDDINSIVELLGSMIEPKGKRDFENINFLPMDILRKQIKAKQMKNILK
                     IAPIVILILAIPYLCLIFSNNLANKELENVNGEIRNIESAYYQIGNIEKMIQSKSEEI
                     KVYDMLIGKKVKWENLLDDIDKNTPSKVELTSLSTTYQNLGTNNKADNSKSNKTTTDK
                     SSNNETKSSTKSDSNGDKTDNDLNEDKTTNVSEALTGNSETNSNNTDKDDKTPLYDKI
                     PNVINIEGNAKDSSYVGQFLYKLKGLYYFKDVKLHSIKSNAEDSNYTFSMTLYLKEGV
                     LTNE"
     gene            19839..20747
                     /gene="pilO"
                     /locus_tag="PC414_000557"
     CDS             19839..20747
                     /gene="pilO"
                     /locus_tag="PC414_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HDF2462970.1"
                     /translation="MNKDIFKKDIKDIFKMEVSLKGILIKVIILLAILSYGYFGLYPQ
                     YQKYIATKNSVKQATEKLNIYKEKTALMPKYEKQLEEVESELKEKSKNLLYDMQDGLF
                     LIGLDKKMKAYNIDLTSYDVGNVKEYEHFYGISTTISVRGDYRNVKKLMAYLEEQKNV
                     TQILDYTMTSYIEPEKEEEETTNNKDLIKNVNINKDTKIYWVEGENKWHLYKDCPLIK
                     EGEILKNDKLLDRSTNYLCNTCVLRAATEKEAKKEVVAPKAEGVVDATFKFIMYTKEN
                     TTMILDTDNPSSWRPGKYNPFKSSAK"
     gene            20774..21340
                     /locus_tag="PC414_000558"
     CDS             20774..21340
                     /locus_tag="PC414_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDF2462971.1"
                     /translation="MSLYKNNEKGLTLLEVIIAVFILTIVLSISYKVFNGITSAVKKQ
                     QIITDAQVNINLINKYLNRDLENCKELTKTGSGNNYEYNIEMPDNVVKYEVSIETKKN
                     TEVYSVTRIQNNTIDTENEVREEIIYNQPLVQNNKEMKETPFKIEKQTGKSIYTVSIY
                     YNESVQESHKNSNLNNKTYTFDVMSRIG"
     gene            21377..21904
                     /locus_tag="PC414_000559"
     CDS             21377..21904
                     /locus_tag="PC414_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDF2462972.1"
                     /translation="MSKKNSERGFSLIEVLVAMAIMGIVLFAFFNIINTNNKANTKND
                     TDITSLNYVQSEIENLREKIKSGEFDFDSLDKLEDGTVVYEKLIDKSKKVVYDKVLSE
                     GDVSLYDTPYEKITTIKDEDGNLIDKGNITNKIKTIVEDKSGQIYKIAVTGKSMNDYS
                     SKKEVKIVTEIFKDK"
     gene            21925..23463
                     /locus_tag="PC414_000560"
     CDS             21925..23463
                     /locus_tag="PC414_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2462973.1"
                     /translation="MRKWNKFKSERGAALVLVLVVVALLSIVGLIFSNQIANRIKSTK
                     TTNEGIQAKYLAETCVENSIDKAYEKLYDELEKMDNEFKSENQEKSISRSKLRDISDE
                     DFNNQDEENIEAERLGYMNNINFYLNKASSDLEKASMELKKLYDLYMLDYRDIEYVDA
                     NIISHRDSILEICKNYTSGDISKINEYILKEDIDSTTLIEAKLVNNDILLKMFLEENK
                     IENEHLNSAFSHTYKALDNISLAMQNMIEYRHTFHIDEPKVEVSNGIPDSQQYYELIQ
                     NPIINSMEYIWNSKWDTLEKLLEILPNQTQGFNSLRVHLRNNVRKFEKLSDNISSGKK
                     NTAKNFLKYKELLYEISDQCNQLKSMSYEKIPVKYDNMALITTFDYIQNELLAEIKCR
                     LKELKPQEIDKTEGITIKIPFYKADYDMTKEGWPKLKENGSGAELSLMVTGDKDGIKE
                     VEVTDGKKNIIGLGVEENSNSKYKVDAIVNFNLNIDTNVVGNYDIKDKILINHDISSY
                     KKVN"
     gene            23563..24645
                     /locus_tag="PC414_000561"
     CDS             23563..24645
                     /locus_tag="PC414_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HDF2462974.1"
                     /translation="MYIYDLLEQGIRLNASDIHITVGTNPVARVKGGFVKLSEQILTS
                     EVTMQMAKDIAGESMFKVIEEHGEADFSASLKTGERFRVNAYRQKGNYAIAIRTITAE
                     IPTFEKLGLPESIKSFTEKHKGLVLVTGPTGSGKSTTLASMINIINEKQQKHIITLED
                     PIEYVHHHKQSLVNQREVGTDTESFHSALRAILRQDPDVILIGEMRDPETVSIALTAA
                     ETGHLVFSTLHTVGAAKTIDRIVDMFQPSQQQQIKTQLSTVCEGVVSQQLLPTADGKG
                     RIAAIELMFATPAIKNLIREGKTYQIPNMIQTGVKVGMKTMDQDLMELYKNGKITKDM
                     ALSRCTDQEFMTRMIGGVNYNGYYNR"
     gene            24626..25408
                     /locus_tag="PC414_000562"
     CDS             24626..25408
                     /locus_tag="PC414_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDF2462975.1"
                     /translation="MDIIIVNLYVFMVGIVFGSFFNVCIFRIPEGISISRPPSHCMSC
                     NTRLKPIDLIPVLSYLMSGRKCRYCYEKISSRYAIVELITGLLFLTVFMLYGISTSTI
                     YYLVLVSLLIIITFIDIDHFIIPDKILVFGAIFSMIFNLLFKEIPVKDSILGVFICGG
                     SVWIIVLLIEFVIKKECMGGGDIKLFAMLGFYMGVKNGLLTALLSVYVGAVYGICVII
                     ISRIKGKEYNSVIPYGPFISIGALITILCGKQLLELYFSMVI"
     gene            25510..26160
                     /locus_tag="PC414_000563"
     CDS             25510..26160
                     /locus_tag="PC414_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDF2462976.1"
                     /translation="MEYIIIISLFFAHLINKSLCVFCDREKKNLYNTFFIYIITVVLS
                     VLIYLKFGISVECVQYMVLIPFLIIISIVDYYTIYVYDITIVSGIIVQGIIFLVFFNI
                     VQFDYINHIFGLLIGFIIPYIIAKLTKGLGYGDVGLYSLCCFALGNNYGFYVIVLSFI
                     LAFLFFIILYITKIFKDVDIRKQIPFTPFISLSTILIIFTEYNIFNFYYNLLYKFI"
     gene            26174..26734
                     /locus_tag="PC414_000564"
     CDS             26174..26734
                     /locus_tag="PC414_000564"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDF2462977.1"
                     /translation="MYVVVGLGNPGKKYEKTRHNVGFDVIDILAKEYNISVTKIKHKA
                     LIGEGRVGTEKVLLVKPQTYMNLSGETLIDIYKYYKVDLSNIVVVYDDIDLEVGKIRI
                     RKKGSGGTHNGMKSITKCLGSNDFPRVRVGVSKPEAGQDLADFVLSRFRKEESDNINE
                     ALEKAADAIDSIIRENIDMSMNKYNG"
     gene            26751..30137
                     /gene="mfd"
                     /locus_tag="PC414_000565"
     CDS             26751..30137
                     /gene="mfd"
                     /locus_tag="PC414_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HDF2462978.1"
                     /translation="MNDVFLYPLQNSKEYKDIINCIKNTKGSLLVNGLLPVQKPHISY
                     SIFNDLSRQMIFITSSDLEAKKVYEDLSFYMEDKVEYLGFQDIYFYHLDAKDRNEEAK
                     KLKVLLKLANKEKIILVTSIEAVLRKYIPKQVLLDSVSHYKVGDSLDLEKLTEKLVSL
                     GYERVSKIEGFGQFSIRGGIIDVFSLEYTNPIRMELFDDEIDSIRTFDVYSQKSIDKL
                     QQFSITPSREFIYPEKTTDALVKLKKETTKNTDEGTFQNIDYISSKTYFEGVENYIDY
                     IYPEENKSIFTYLADDAIVFINDITRLKERCENYINEFRENYKLNLERGLAIKNQGKL
                     LYHYTDLEYLVKDKSVVLNSLLPKSINNFSIKSIINFESREVPTFNGKVDLLVEELNR
                     LKYNGYKIILATNTLERANKLGKDLLDKGLETTISKDRDIEIKSSQVIIVPAHINSGF
                     QYKSIKFVVITDNEMIGVYKRASKTSNKKVKKGKKIESFLDLSVGDYVVHENSGVGRY
                     TGIEQITVNAIKKDYMKIVYQGGDNLYVPIDQMDKVQKYIGAEVEKVKLNKLGTNEWT
                     KAKAKVKKEIEDMTKDLIELYAKREKIQGYKFSKDTPWQAEFESLFPYQETEDQLKAI
                     EETKKDMESSKVMDRLVCGDVGYGKTEVAIRSIFKACMDQKQVAVLVPTTILAQQHYN
                     TFKERFENYPLRVEVLSRFKTPKQQKQIIEDAKKGLVDILIGTHRIISKDINLPNLGL
                     VVIDEEQRFGVKHKEALKKIKSTVDVLTLSATPIPRTLHMSLSGIRDMSVIEEPPQER
                     HPVITYVTESKESVIQDEIERELSRGGQVFFVYNRVEHIEEMASMIQKLVPDARVAVA
                     HGRMTSKSLENIILGFLNKDYDVLVCTTIIETGMDISNANTMIIYDADKMGLAQLYQL
                     RGRVGRSSRQGYAYLLYEKDKTLSEIAEKRLKAIREFTEFGSGFKIAMRDLEIRGAGN
                     ILGSQQHGHMAVIGYDLYVKMLNDAIKKVKGEPIVEEIDVEIDLSVNAYIPDNYIKDE
                     LIKIEMYKKIASIENKEDMLDIQEELEDRFSDIPKPVQTLLTIAYIKSLCKILKIEKI
                     RQLKDEILLVPITKYRTKQKIGYNIVTELEELLEKMCKVK"
     gene            30168..31163
                     /locus_tag="PC414_000566"
     CDS             30168..31163
                     /locus_tag="PC414_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF2462979.1"
                     /translation="MKKVITLVIAMILVVSVTACSSSKGETVATVEGTKISSDEFKKT
                     IALYKDSMEQTYGKDIWDKEVEKGVKYKDKFKDLILDQLITTEVIYSQAKKDNLLPKK
                     EDVEKSFKELKDAMGKDEKYKEQLKKLGIDDEFLRDQQEKDLAMQNYQSNFAKKTKIS
                     DEEMKKYYDTHKDEFKKDEVEASHILLKTVDDNNKPLSDKEKAEAKKKAEEALKEVKS
                     GEDFAKVAKKYSQDTSASDGGKLGFFSRGQMVAEFEDAAFSMKKGEVSDLVETQYGYH
                     IIKVTDRINEQTSFEDAKETIKDQLLKNKYQEQIEKLTKEAKVEKDEKVINKITI"
     gene            31287..31826
                     /gene="spoVT"
                     /locus_tag="PC414_000567"
     CDS             31287..31826
                     /gene="spoVT"
                     /locus_tag="PC414_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein T"
                     /protein_id="HDF2462980.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRKTLRIREGDPLEIFTAKDGEV
                     ILKKYSPIGELNEFSQEYAETLAEVLGYGVLITDLDSIIAVSKLPKKDYKEKSISDEL
                     EAIISERTVKYSKDKNILPLFKEDQKEYTSQVIMPIISASGDCIGSIVVVSKDKDTIA
                     ESEEKSLKIAANFLGKQVQ"
     gene            31946..33559
                     /locus_tag="PC414_000568"
     CDS             31946..33559
                     /locus_tag="PC414_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDF2462981.1"
                     /translation="MNNNKNKDSFLKGALILGLAGVIVKIMGGFFRIPLGNMIGSEGM
                     GYYQAAYPVYTLFLTLATAGFPTAVAKLVSEKVAIGNFKGANKIFKVSHTVLFITGII
                     SFCILFFGADYIVTNVMKNPGALYSMKAIAPALLFVPAMSAYRGYFQGRQDMTKIAVS
                     QVAEQFFRVVLGLTLAYLLMKSLGQTFGAAGAISGATIGSIASMLYLVFAYMLGRKER
                     RAEIDASQRFKDERVSYIFKKLLTVAIPITIGASVMPLVNMIDNVIVIRRLMEAGFTY
                     KVANSMFGQLTGMAMATINLPAVITTAMSMSLVPAISKAYALGNKSKARKDTKSAVKV
                     TLLIVLPCAFGMASLAIPIMGLLFPHEPSSVGTILFTLTPCVLFLGLIQTLTGIIQGM
                     GKPIVPVIALCVGMLCKIVISYTLTGIPDINVLGSAFGTVTAYFVAAMINLLYVKKHM
                     NVNFSKKEFIIKPFITVMTMFIMVKLSYGALVGFLGNSISTIIAICVGGIVYVVVILG
                     IGGIKKEEILTMPKGDKLYKLLKKVKLIR"
     gene            33666..35114
                     /gene="mazG"
                     /locus_tag="PC414_000569"
     CDS             33666..35114
                     /gene="mazG"
                     /locus_tag="PC414_000569"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HDF2462982.1"
                     /translation="MGKISIVGLGPGDYSLISQGVLESLINSPKVFFRTEKHPIIDKL
                     KETIQYTSLDYFYEKEENFDNVYLKISEFIIEESAKGDLVYAVPGHPRVAEKTVSIIE
                     RLANEKHIAVEILPSMSFVDAMFNYLAIDPSEGFKLVDAFEIENSYIDLDTSMIITQV
                     YDKFIASNVKLKLMEHYDYNQEVCIVNGAGVKDLESKKFIKLCELDRNENFFDYLTSL
                     YIPKSSKKMYNTVHDLEIIVNTLRSPSGCEWDKKQTHQSLKNSVIEEAYELCNAIDNN
                     DIDEMVEELGDVLLQVIFHCQIGNEEGYFDLKEVVNGICKKLIHRHPHIFSGAKLDMS
                     KFEKTWEELKKEEKGESTITDGLRRIPKHLPALMKASKVQHKAALVGFDWDNVEDVFK
                     KIEEEYQELLDEHRVGNIKYIKEELGDLLFSIVNLARFLDIDSGEALNCTTEKFINRF
                     DFIEKSAMNLNKKLEDMTLEEMDEFWNQAKKQ"
     gene            35203..35481
                     /locus_tag="PC414_000570"
     CDS             35203..35481
                     /locus_tag="PC414_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003153447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HDF2462983.1"
                     /translation="MNKAELVSKMAEKSGLTKKEAEAALNAFMSSVQDALVNNEKVQL
                     VGFGTFETRERAARQGRNPRDPEQVIDIPASKAPVFKAGKGLKDIING"
     gene            35553..35795
                     /locus_tag="PC414_000571"
     CDS             35553..35795
                     /locus_tag="PC414_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HDF2462984.1"
                     /translation="MRLDKFLKVSRIIKRRTVAKDACDKGIVTINGKVAKSSSEVNIG
                     DTLEIQFGEKKMKFKINEVKEHVLKNDAKEMYEILE"
     gene            35857..36111
                     /locus_tag="PC414_000572"
     CDS             35857..36111
                     /locus_tag="PC414_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HDF2462985.1"
                     /translation="MEQNITLKDRSKLVISGVEHIYSFNDKRVELKTSVGEMVIEGEN
                     LDMSKLSIDENIISIDGTINSMVYAKPKKPQESFLKKVFR"
     gene            36112..36645
                     /locus_tag="PC414_000573"
     CDS             36112..36645
                     /locus_tag="PC414_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2462986.1"
                     /translation="MIPFTQDVSIFYATIYGGILIGVLFDFYRGLRGNFKFINYFAII
                     FDVLFWFLATVIIFVTINLTEFFDLRYYHFVALFIGFILYYNTISKIVLSIINKIIRF
                     VRNSFKKVTHYIVSFLNNLYYVIIYSLHLLFDIIFYIPNIFIATRKSIKRKSNKKLKN
                     KKKSKPKKKKNKTKKRV"
     gene            36651..36935
                     /locus_tag="PC414_000574"
     CDS             36651..36935
                     /locus_tag="PC414_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HDF2462987.1"
                     /translation="MNLRKKFSGQIIVISLFLGISIFSMMTGFVFEYTKAKEYKKEIA
                     SLNKQLKKTEIQINSLKKDEKSYEGDLEDIARKRLNMVKPNETVYVDINR"
     gene            37048..37968
                     /locus_tag="PC414_000575"
     CDS             37048..37968
                     /locus_tag="PC414_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HDF2462988.1"
                     /translation="MKVGAIDIGTNSMRLLIADYRDGNFINRKKFVNTTRIGYGVDSK
                     GYIKEDAIKRNIKALKEFSDKCNDEKCEKIYCMGTSALRDSKNGDYFVKLAKEETGID
                     VDIVTGIEESNLGFKGVLEGIQGDDTIIVIDIGGGSTEFIVGDSEGIIFNKSENVGAL
                     RMTERFLKHDIIEDEEFEAMSSFIYGEIDKTLNHIKDKGLKTLIGIGGTITSLSAMNQ
                     ELEIYSMEKIHNSKIHKKEIEEILQKLIKMTLSDKKNLKGLQEKRADIITAGVKILNI
                     IMEKLEIEEITVSEYDNLEGLICQKSKKMY"
     gene            38058..40430
                     /gene="spoIIE"
                     /locus_tag="PC414_000576"
     CDS             38058..40430
                     /gene="spoIIE"
                     /locus_tag="PC414_000576"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein E"
                     /protein_id="HDF2462989.1"
                     /translation="MQRSVAVVKNKTVKPNVIKLISGYIDFTTAAFAVMGFLLSRSII
                     VDSVAPLGIAFFICASKIDKYKIPVFISTLLGILLSSNDFGNVAKYAVCLTIFMAMNK
                     KLKLSDSIAKVAFIGAIILLPISMGQAFFSNRYVYGLLIAGVEAIIMFISIYVFSYGI
                     GLMANVNNRMSVRTEEAISISLLLTFSIMGIGDISLFGISIRTILSTVLILLTSIVGG
                     ATMGASSGVIIGVASILNNMTSAVYMGIYSFSGLISGAFNKINKYFCILGYILSWTII
                     YLYTSGITSNMMQLRDILLGCLIVLVLPERLFNKIEKLIKSNVASNEIVYDYIMRSKN
                     LTNSRLNSIYKTYDDLADTFDKIREKDKVLDQRDIANVIDMIHNDECKSCSMRRMCWE
                     SRFNHTYTMIYNILEKIEEKGELSLNDIPKNFRKECMKPESIVKISNHYYKMFVLDYD
                     WSVKFSESRKLIANQIRSISKSIKSLSQDLEGDIMLDIEKEKNIYEQLERYDITVDKV
                     SYLTKSNSEFEISIEKKTCHDGCMCEDKIVNIISDLVGENMSVRKIGCHCLGGKCKAT
                     FVKSQKYKAVTEVSAMSRDGHILCGDNYTYMEINDGKYMMAISDGMGKGKKAYEESSA
                     TIDILEKMIDAKIKDEIVIDTINNMLLLKSSEEMFSTLDLGILDLKRGCLETIKMGAC
                     STYIKREDGEVDLISSSSLPVGILSDVKIDRKNVKVKEGDYVIMVSDGIVDAGRNNNL
                     GDNWLIYFLKNIETTNPKEISNLILDRALELQALQIEDDMTVLVTKICTK"
     gene            40781..42673
                     /gene="pepF"
                     /locus_tag="PC414_000577"
     CDS             40781..42673
                     /gene="pepF"
                     /locus_tag="PC414_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HDF2462990.1"
                     /translation="MNRKLCKAIIPLFISIFCMLIISIKADQGYQKNNDRAITVMNNN
                     DTEDDAEKKQEIDYEKTCWNLQSLFKSDDQWKKELKSFDKDTKELKNYIGKVTKSPTH
                     LSFALNIKEKLDIRLNKLSAYVKLKQDTNKNSYKYLDMNDSMSKSYGNYMGICSDLEL
                     EILKLSNKDYKKIISNKNINRKYGAYLRDIRRNKVHYLDDKSEDILSKVSSISSLPSQ
                     VYELFRNMDRKTNLTPAQYASELESSDRENRKKAYQDELITYNDNINTIAGLITGQVK
                     KNIFYSQERGYESSLEMYLESDNVDEKVYNNLIRTVNSNMDSLHKYIDLRKKVLKLDK
                     VHSYDMFVPMVKAVDNNINYEKAQSIVYSALSPLGKEYNDVVYKAFNEKWIDVYSHEN
                     KVSGGYCLSVYENHPYILLNYNNSLGSVSTLSHELGHAVYEYLSSKNQNYFNSNPSIF
                     THEVASTTNEALLYEMLIKEAKGNDEKAYYITQYLDLIKDTLYTQTMYAEFEKTIHEL
                     VEKGKSVNALVLNDVWGQLLKKYYGQSYELDQLSKVGWARIPHFYNSFYVYKYATGCS
                     AGVSFAQDILKNGSDNYMSFLKKGSSDYPINLLKSSGINLTNTKPIENTIKKFDSLVA
                     ELEKLL"
     gene            42787..43752
                     /gene="galU"
                     /locus_tag="PC414_000578"
     CDS             42787..43752
                     /gene="galU"
                     /locus_tag="PC414_000578"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDF2462991.1"
                     /translation="MQVKVKKAVIPAAGLGTRFLPATKAQPKEMLPIVDKPTLQYIIE
                     EAVASGIEEILIITGRNKKSIEDHFDKSVELELDLEKKGKKELLEVVQNISNMINIHY
                     IRQKEPKGLGDAIYCARHFIGDEPFAVMLGDDIVDNDVPCLKQLTDAYEEYRTTILGV
                     QKVNQEDTNKYGIIEAKNIEGRVYKVKDMVEKPESGKAPSNIAILGRYIITPEIFDIL
                     KDLPPGKGGEVQLTDALKILSKKEAMYAYNFEGKRYDVGDKLGFLEATVDFALKKEDL
                     KEDFIKYLKHVCSEFDKNNNVLYNNTDEKIDLIEVNEARELEIQK"
     gene            44252..45856
                     /gene="rho"
                     /locus_tag="PC414_000579"
     CDS             44252..45856
                     /gene="rho"
                     /locus_tag="PC414_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HDF2462992.1"
                     /translation="MRRIFLILDDKVLEGLGSKTLVELREIAKELKIKSITTYKKNEL
                     IEIINSKSKNEEKVNEETKEKNEDIKEDIKNRKENIERESIEKEVEYKSPTKSYNKKE
                     IDTQNISQNQEHRNQRNDYNKTTSVNDSNKSSNISNRMVRNNNKNYYMPKQVDESKIV
                     DEFNTSKEDEVVGVLEILPDGFGFLRGSNYLSTEGDVYVSPSQIRRFNMKTGDKIKGI
                     TRHPKSGEKFRALLYVQKINDENPDTAIQRNAFETLTPIFPEERLTLETNRNEIATRI
                     IDLISPIGKGQRGLIVAPPKAGKTVLLKSVANSIAKNHPNVELIVLLIDERPEEVTDM
                     KESIEGDVIYSTFDQVSSHHVKVAEMVLNRAQRLVEHGKDVVILLDSITRLARAYNLT
                     ISPTGRTLSGGIDPGALHGPKKFFGAARNIRQGGSLTILGTALVETGSRMDDVIFEEF
                     KGTGNMELHLDRKLAEKRIFPAIDIYKSGTRRDDLLLDDEEKTALWRLRREMSNNSVM
                     EITDKVIELIKRTKDNKEFVKSIKNL"
     gene            46009..46209
                     /gene="rpmE"
                     /locus_tag="PC414_000580"
     CDS             46009..46209
                     /gene="rpmE"
                     /locus_tag="PC414_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018591942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HDF2462993.1"
                     /translation="MQKEIQPKYNPVEVRCACGNTFVAGSTKDEIKVEICSECHPFYT
                     GKQKNIEKGGRIDKFKKRFKMD"
     gene            46354..47247
                     /locus_tag="PC414_000581"
     CDS             46354..47247
                     /locus_tag="PC414_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HDF2462994.1"
                     /translation="MAKQAVGGQAVIEGVMMQSKDKRAVAVRKSDGEIALKEDRIKSW
                     VRDKNIDKIPFVRGSFVMIDTMIQGIKSLNFSSEFFMEEAEEDKFDLFIKKIFKDKAN
                     DIIIIFSLVIAMLLSAGLFIFIPTLVGGAFSKVMPNDFMLNLIEGIIRIAILFAYIVL
                     ISRSKDIERVFQYHGAEHKSIYCYENDLELTVENARKFKRLHPRCGTNFLFIVMAVSI
                     ILFAFFGWPNPILRIFMRIICVPIVAGLSYEVIRVLGKYDNGFTKIIAYPGMMLQYFT
                     TKEPDDEQLEVALEALKAVVD"
     gene            47265..48113
                     /gene="prmC"
                     /locus_tag="PC414_000582"
     CDS             47265..48113
                     /gene="prmC"
                     /locus_tag="PC414_000582"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDF2462995.1"
                     /translation="MTIKDIIIKYSDKLKDISDTPRLDTELLLQKTLGVDRLYIHLNL
                     NKELTEEQKTKFMGFAEERLNGRPIAYIVENREFMGLDFFVKEGVLIPRPDTETLVEE
                     IIEICREKKDVSILDIGTGSGAITISLAKYIENSKIMSFDISETALEIAKKNAIINEV
                     GEKIKYINSDLFTAISDSNIKFDIIVSNPPYIKKQDIETLHKQVKDYEPYNALEGGED
                     GLDFYRRITEQGKKYLNKCGILAYEVGHNQAEDVINIMKSNGYKKIYTKKDIQGIDRV
                     VIGYNI"
     gene            48157..49221
                     /gene="prfA"
                     /locus_tag="PC414_000583"
     CDS             48157..49221
                     /gene="prfA"
                     /locus_tag="PC414_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HDF2462996.1"
                     /translation="MLKKLEVLEDTYKDLSEKIGDPDVINDQKVWQKYIKEHADLEPI
                     VMKYREYKSVLDSIKESKEILQEESDEELRELAKMELAEMEEKVAPLEEEIKILLLPK
                     DPNDDKNVIVEIRGGAGGDEAALFAGDLFRMYSRYAERRRWKIELLSASDTGVGGYKE
                     VSFMIKGKGAYSRLKYESGVHRVQRIPSTESGGRIHTSTSTVAVLPEVEDVEVEINPN
                     DLRIDVFRSSGNGGQSVNTTDSAVRVTHIPTGEVVSCQDGKSQLKNKEQALKILKARL
                     YDKALAEQHKDIAAERKSQVGTGDRSERIRTYNFPQGRISDHRINLTLYKLDAFLDGD
                     IDEMIDALITVDQTEKMTAI"
     gene            49350..50030
                     /locus_tag="PC414_000584"
     CDS             49350..50030
                     /locus_tag="PC414_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HDF2462997.1"
                     /translation="MILKVTIIGLLAGVIGTGLGGVISAIFKREVDKYLSFFMGLSGG
                     IMLAVVVFDLMKESMDKMGIINTVIFTFVGALITMYIKTKLDVSGNMASGYLIFISIL
                     LHNLPEGLAIGSSFMSTESLGITLAIVIGLHNIPEGLAMALGLVCNKMKLSKVILFTV
                     IAGLPMGLGSFLGVYFGGVFTSLIGVFLATAGGTMMYVVLEEIFPHSKSVYCIIGFLL
                     GTMIVNYI"
     gene            50059..51099
                     /locus_tag="PC414_000585"
     CDS             50059..51099
                     /locus_tag="PC414_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HDF2462998.1"
                     /translation="MKTIISNIDINNIDYEEIKIQAKLLREGKTVIFPTETVYGLGAN
                     ALDENAVKKIYEAKGRPSDNPLIVHIYEKEEVYDLAKDISDKAKLVIEKLWPGPITII
                     LNKKDIIPYKTSGGLDTVAIRMPSNVIARAIIKEAGIPIAAPSANISGRPSPTKAKHV
                     YEEMNGRVDGIVLGGDSNFGLESTVLDLTEETPMILRPGSITKEVLEELLGEVKLDPS
                     LSKKEDNQKAKAPGMKYKHYSPNADVYIISGKRENVATKINDMIKSNRDKGLKTGVMC
                     ISRNREFYDGEVIDLGNSLEEVASNLFDALIEMDKKGVDVIYSEEFPKTGVGQAIMNR
                     LLKSAGYKVIKA"
     gene            51116..51565
                     /locus_tag="PC414_000586"
     CDS             51116..51565
                     /locus_tag="PC414_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HDF2462999.1"
                     /translation="MNILIVCTGNTCRSPMAEAILRKAIKESGRSIEEYSISSAGIST
                     ANGMGASENSIEVLKEIGIDLSNHRSKVITKKLIDESDIILTMTKSHKEILVQAVPKC
                     KEKVYTFKGFANKNEEDISDPFGGNLDIYRSTMREIMYSVNEIVKKI"
     gene            51582..52034
                     /gene="rpiB"
                     /locus_tag="PC414_000587"
     CDS             51582..52034
                     /gene="rpiB"
                     /locus_tag="PC414_000587"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HDF2463000.1"
                     /translation="MKIGLGCDHGGYNLKKEIISYLESKGIECVDYGTNNATDSVDYP
                     VYGEIVANSVINKEVDYGILCCGTGIGISLAANKVPGIRCAVVSDVFSAKMSKAHNDA
                     NMLSLGERVLGKGLALEIVEAWINTDFEGDRHARRVNMIKSIEEKHNK"
     gene            52070..52699
                     /gene="upp"
                     /locus_tag="PC414_000588"
     CDS             52070..52699
                     /gene="upp"
                     /locus_tag="PC414_000588"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HDF2463001.1"
                     /translation="MSKVVETNHPLIQHKLTLMRDKNTGSKDFRELLTEIAMLMGYEI
                     TKDIPLKDVEIETPIQKTSSKVVAGKKLAIIPILRAGLGMVDGLVSLMPAAKVGHVGL
                     YRDPETLKPVEYYCKLPQDIGERDIIVVDPMLATGGSAVAAIDLLKSKGAKSIKLANL
                     VAAPEGIAEVQKYHDDVDIYVASVDERLNEHGYIIPGLGDAGDRLFGTK"
     gene            52994..53809
                     /locus_tag="PC414_000589"
     CDS             52994..53809
                     /locus_tag="PC414_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HDF2463002.1"
                     /translation="MKNCIVVRGAGDLATAVIHKLHNSGFKVVALEIEEPLAIRRQIS
                     FCEAVYEKKVTVEGVECIFCNTEKEIEEVLSQHKVALIIDPKGEKLSKLNPIVLVDGI
                     LAKKNLGTTKDMAPLTIALGPGFCAGEDVDVVIETMRGHNLGRIITNGRAEKNTGVPG
                     AIKGFSKERVMHSQYDGIMENVCKIGDIVEKGDLLCYINNYEKKHEVRATISGVLRGL
                     LKDGAKVNKNLKILDIDPRKEEVKNAFTISDKARCIAGGVLEAILNNGIYPQN"
     gene            54035..54472
                     /locus_tag="PC414_000590"
     CDS             54035..54472
                     /locus_tag="PC414_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase"
                     /protein_id="HDF2463003.1"
                     /translation="MRPSWDEYFMEIAEVVKKRSTCIRRQVGAVIVRDKQILTTGYNG
                     SPRNLEHCENIGCKRQELNIPSGERHELCRALHAEQNAIIQAAHNGISVDGATLYVTT
                     RPCVLCAKMCINAGILKIVYEGDYPDDMSTELLKEAGIELIKF"
     gene            54657..54884
                     /locus_tag="PC414_000591"
     CDS             54657..54884
                     /locus_tag="PC414_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HDF2463004.1"
                     /translation="MSKRNTHTEIAKLFSLISQIGLMIVISILGCTFLGKFLDSKLNT
                     TPVLTIIFLLLGVGGAFSGVYKTLIVYTRRK"
     gene            54889..55275
                     /locus_tag="PC414_000592"
     CDS             54889..55275
                     /locus_tag="PC414_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HDF2463005.1"
                     /translation="MDIKLLNQVRSVTKGIVIFDVVLIVLMLITSTLSKPVLLGLIFG
                     SIIAMLNFRLLAISLEKSVTFPAGKAQAYASAQYAVRLFIMAVVLIASATVPHLNIIG
                     TALGLLSTKFVILSKNFINKLKRKEA"
     gene            55278..55982
                     /gene="atpB"
                     /locus_tag="PC414_000593"
     CDS             55278..55982
                     /gene="atpB"
                     /locus_tag="PC414_000593"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HDF2463006.1"
                     /translation="MNQAHYYLIGNFKISETILVSWGIMAFLAIASYFLTRNLKKVPT
                     SKVQIFLEFAVGGLAKMVTDVMGKNTVKRMPYMIPYIGSLFLFFACSNLSPLLGLRSP
                     TTDLDTTAAWAMITFFMIYFAGVKFKGLSYFKELAQPLAIMLPINIIGELARPISLSF
                     RPFGNILGGTIIMGLFYQLLGFISSLIPGLSIPIGEILIPVPLHLYFDLFAGLLQAYI
                     FIMLTMVFISSAGELD"
     gene            56051..56311
                     /gene="atpE"
                     /locus_tag="PC414_000594"
     CDS             56051..56311
                     /gene="atpE"
                     /locus_tag="PC414_000594"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HDF2463007.1"
                     /translation="METAIVAAASAIGAGIAVATGIGAGIGQGIAAAKAAEAVGNQPE
                     AKGDITSTLLLGVAIAESSAIYGLVISIILLFVNPFFKYLGM"
     gene            56434..56931
                     /gene="atpF"
                     /locus_tag="PC414_000595"
     CDS             56434..56931
                     /gene="atpF"
                     /locus_tag="PC414_000595"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HDF2463008.1"
                     /translation="MEKALVGITWEFVFQIVNTFIIFLLLRKLLFKPVLNIIESREND
                     IKSDLAEGEKAKNEGLALKKEYESKINFAKDEGQEIIKQATIRAEQKSDDIVNTAKKD
                     ALDIKEKANKDIEQERQKVINEIKNDISNIALLAASKVIEKDLDKSKHEELIENFIKE
                     VGEAK"
     gene            56928..57473
                     /locus_tag="PC414_000596"
     CDS             56928..57473
                     /locus_tag="PC414_000596"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HDF2463009.1"
                     /translation="MINVIANRYAEALFQLGEEENSTDVLFKELEKVVDMMTKVSKDF
                     YKVLKSPLVSKSEKKNLVEIIFSKEVSSNIKNFLKVLVDKDRISYLEDIELAYKELLN
                     KKNNVIDGVAISAIPMSETDIKELEVKLSNKYNKNVTIENVVDKTILGGVLVRIGNEQ
                     IDGTVKTRLDKMKEKLSEVIS"
     gene            57490..58992
                     /locus_tag="PC414_000597"
     CDS             57490..58992
                     /locus_tag="PC414_000597"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003405060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDF2463010.1"
                     /translation="MNLKPEEISSIIKQQIKNYENKVELTDTGSVLTVGDGIASVYGL
                     EKAMSGELLEFPGEIYGMALNLEEEVVGAVILGDDSEIKEGDIVKRTGRIVEVPVGEA
                     LIGRVVNSLGQPIDGKGPIAYTKTRPVESEAPGIIDRRSVYEPLQTGIKSIDSMIPIG
                     RGQRELIIGDRQTGKTSIVIDTILNQKGKDVICIYVAIGQKRSTIAQLVSSLEKGGAL
                     DYTIVVSATASESAPLQYIAPYAGAAMGEEFMYNGKHVLIVYDDLSKQAVAYREMSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDELGGGSMTALPIIETQAGDVSAYIPTNV
                     ISITDGQIYLQPELFYSGVRPAVDPGISVSRVGGSAQIKAMKKVAGTLKLAYSQYREL
                     AAFSQFGSDLDEDTKKRLAQGERIVEILKQGEHQPIKVENQVMIIYAVINNHLEDIPI
                     DNIARFESELYAFVDNNYPEISRKILGGEDFTHDLTDAINEFKEKFVVEV"
     gene            59020..59898
                     /gene="atpG"
                     /locus_tag="PC414_000598"
     CDS             59020..59898
                     /gene="atpG"
                     /locus_tag="PC414_000598"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HDF2463011.1"
                     /translation="MAGAGIKAIKTRMGTVESTKQITKAMELVASSKLRKAKEKAEDS
                     REYFNTLYNTVKDIAQTTRGIRNEFLKEREVKNKCYVVVAGDRGLAGGYNSNVLKESV
                     SHMKGKKETVITVGKKAHEFFSKRNYNIAKDINSVEACGYEDATEISQTIIDLYKKGE
                     VDEVYIAYTKFKSALSQEVEILKLLPLVFEKDTDEQGMAVKEEKQNRSKVQYIPSAEA
                     VLSYIVPQFISGTVYGGILESFASEQGARRTAMESATDNANEMLSNLELSYNRARQSA
                     VTQEITEIVGGVEALK"
     gene            59911..61305
                     /gene="atpD"
                     /locus_tag="PC414_000599"
     CDS             59911..61305
                     /gene="atpD"
                     /locus_tag="PC414_000599"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HDF2463012.1"
                     /translation="MANVGKVVQIVGAVLDVKFDSEQSLPNLLNALVIKLGDKEIVAE
                     VAQHIGDDTVRCIAMSATDGLVRGMEVVDTGGPISVPVGDETLGRIFNVLGKPVDGKP
                     APKSAPKLPIHRPAPAYDELETTAEILETGIKVVDLLAPYLKGGKIGLFGGAGVGKTV
                     LIQELINNIAKQHGGISVFSGVGERTREGNDLYGEMSESGVINKTALVFGQMNEPPGA
                     RMRVALTGLTMAEHFRDEQGQDVLLFVDNIFRFTQAGSEVSALLGRMPSAVGYQPTLA
                     TEMGALQERITSTKKGSITSVQAVYVPADDLTDPAPATTFSHLDAKTVLSRQISSLGI
                     YPAVDPLESTSRILDPSIVGKEHYEVARGVQSILQRYKELQDIIAILGMDELSDEDKL
                     IVARARKIQRFLSQSFTVAEQFTGNPGQYVPVKETVRGFKEILEGKHDDLPESAFLFV
                     GTIEDAVRKAKGSM"
     gene            61308..61568
                     /gene="atpC"
                     /locus_tag="PC414_000600"
     CDS             61308..61568
                     /gene="atpC"
                     /locus_tag="PC414_000600"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HDF2463013.1"
                     /translation="MASEFSLKVVTPSNTFYDGDVEMIVVRTTKGDKGVLKGHIPFVS
                     GIPVGTLKIKKEGSFKEAKIAEGFINVTQDKTVILTESAEWI"
BASE COUNT        25045 a         6577 c        11485 g        18815 t
ORIGIN      
        1 taaataagat attaaataat tataaactaa agtaaataaa atattgagcg attattaaaa
       61 agatatatat ataatttaat ttgagcagtt tgttatatat tataaaaaaa gagaaaaata
      121 aaaggtaaat tgtttctgga taacttaaaa ttggaagtaa gaatatttaa ctagagaaga
      181 attatttaag tttaggttag ctctaggagt agctgatttt ataggaacaa tagattttat
      241 ggatgatgat ataaacatgg tagcaattag accagaagat ataagggtag aatttaatag
      301 agatttcaaa gaaattatag acctcaaact cagaccgtat cgatataaaa actaaagtta
      361 gatgatatag tgttgtagat aggaccatca gctgttatgg cagtattagt gcttttaaca
      421 aatataatag taaaactttt gtatgaattg ataataaaaa aattaaaaac caagacataa
      481 aaatggcaac aaaaagaagt tgaataaggg aggtaaaaat gaaatttaag aagttagtct
      541 ctttgggaat attatcaaca cttttattga gtgtaataac tggatgtagt aatgtttcat
      601 caggttcaaa cttaaagtct aataataaga caaaaggaga gttaacagtt tatacagcaa
      661 ttgaggaaga tagtatagat acatacttag ctacatttaa agagaaatat ccagatatta
      721 aattgaatgt acttagatta tcagtaggag gtataacagc aagattatta gcagaaaaag
      781 ataatcctaa agcagatgtg ttatggggtg ttgcagctac aagtttgata attttagatg
      841 acaaagaaat gctagagcca tacgctccga aaggctttga taaggtaaat tctgcattta
      901 aagatgataa gaaagttcct tcttgggttg gaatagatgc ttggatgact ggtataacag
      961 taaatactaa agaattaaaa gctaaaaata tatcagctcc tagttcttat gaagatttaa
     1021 taaagccaga atataaagat cttatatcta tgcctagtcc agcttcttca ggaacaggat
     1081 atttaacagt atcagaactc atacaaatta tgggtgaaga aaaagcatgg cagtatatgg
     1141 ataaattgca taaaaatatt ggtgtataca ctcagtctgg ttcagctcca gctatattag
     1201 cagcttcagg agaatatccc attggtatat cttttggaaa tagaggtata aagttaaaag
     1261 gtgaaggtta tccagtagaa gtagtatttc caaaagaagg ttctggatgg gagatagaat
     1321 caaatgcact tgtgaaaaaa gataatataa aagaagcatc aaaagtattt ttagattggg
     1381 ctataagtaa agatgcaatg aatgagtatt ctaagaattt tgcagttact acaattgaca
     1441 cgggaaatcc ggtgccagaa ggatttccaa agaatccatt agaacagatg atagataatg
     1501 atttaaaatc agcagcaaga aatagagagg ctatactaaa taaatggata gaaaaatatg
     1561 atggaaagac agaaaaagag agctaagcct tgcaataaaa tttagtacaa aaagcgccta
     1621 gaatataagt tttatatcta ggctcttttg atatatttat ttaaaatgca tatttctata
     1681 taaaaataaa tagtttagta ccttgattaa attaaataaa aaccttaaaa taaagagatg
     1741 taaagaaaaa ctaaaaacga ataaaaggtg aatttatatg ataaaagaaa taatagtagt
     1801 agagggtaga gatgacgtta cagcagttaa gagagcaata gatgcagaac ttataactac
     1861 aggtggattt ggatttccaa aaggtgtaat ggagcgtata aaagctgcta atgaaagacg
     1921 tggtgttata atatttacag acccagattt tgcaggtgaa aaaataagaa aaaaaatagc
     1981 atcagaagtt ccaggatgta aacatgcatt tctaccgaga gaggaagcta aaaaggatgg
     2041 agatataggt attgagaatg ctactcctca aagtataata gcagctctga ataaagttag
     2101 aactgaaaat acagaaaaaa gagatgaatt taagcaagtt gacttaatta gaaatggtct
     2161 tataggtaat gaagaagcat cacttagaag agatgcactt ggaaaaatac ttggaattgg
     2221 atatggaaat gcaaaacaat ttttaaatag attgaataat tatggagttg aaagagaaga
     2281 atttatagat gcattaaaac aaatctaaac gtaatgttta atgtatgaaa aatatgtata
     2341 taaaataaat tgtggaatta cggttaattt aagatgaatt atttataata tacaccttat
     2401 tttaaatcaa tgtaaataca tataaataag tataattaat aaaattaaat atcaaaataa
     2461 gttaaaaagg agtaaataat ggatagacta tcttcacata atgcaacaaa ggaagtagta
     2521 caaaaatata attttaaatt ttcaaagtct ttaggtcaaa atttcttgat agatagtaat
     2581 ataattgata aaatcttatc tggagctaga atcacaagag gagataatat catagaagta
     2641 ggacctggta taggaactct tactcgtgag atgggtaaaa tagcagaaaa agtagtggct
     2701 atagaaatcg atagaaattt aattcctata ttgaaagata ccttgtcaga tttagataat
     2761 acagaggttg taaatcaaga tatactaaaa gtagacatac aggaattagt aaaagataaa
     2821 ttaaatggtg ggccagttaa gcttgtagca aatcttccat attatataac tactccaata
     2881 gttatgaaat ttttagagga agacatacct gtaacagaca tagtagtcat ggttcaaaaa
     2941 gaagttgcag atagaatgaa tgctattccg ggtactaaag attatggggc attatctata
     3001 gctgttcaat actattgtga tacagagatt gtggcaaaag ctccaagaca tatgtttata
     3061 ccacaaccaa atgtagattc cacagttata ggtctacatg taagagataa aagaaaatat
     3121 gatgttcata atgaagacat attttttaaa acagtaaaag catcctttgg acaaagaaga
     3181 aaaactcttc ttaattcgtt gggaggtctt ggttttctta ataaagatga aataagagaa
     3241 gtattaaaag aagctaatat agacgaaaaa agaaggggtg aaactcttag tatagaagag
     3301 ttttcagttc tttcaaatat aataaataca aaagtatctt ctaaatagaa ggtacttttt
     3361 ttaattttgt taacatatat tgtttagaga aactgtgtta aggggggatt gcagtgggtt
     3421 ctaaaagaaa gtttaaaaga gactatataa tactagaagc taaagacata aactttagat
     3481 acaaagaacg agtactgcca aaagcttttg caaaagttga agtaaatgat gaaaaagcaa
     3541 ttattgcatt atatgtagaa aatctaaaat atgttaaaga tgggtataaa gcagtagcaa
     3601 taaaagatga ccatggatta atagacttag gaaatataat tttaaatgaa caaggaagag
     3661 gtgaatttgt tttagattta gaagattatg atgatgagat aaaaggtatt gcattattgt
     3721 atgaaagaaa tataccttta attggattta aaggaaataa gcttgaaaat tatgaggaca
     3781 tattatttca agaggtagag gaagaagaat atgaagaata tgaagagttg gaagaagaag
     3841 acgaagagat agaggagaat gaggaaagtg atagagacga agatagtgaa gaagaatata
     3901 ttatagaaga atatgatgaa gatgagtatg aagactattg ggaagatgat gaagatattg
     3961 aagaaggaga gttaaaagca agaacggaag tagaagattc tgatgatgag gaaatagaag
     4021 agttcaataa ttataaggaa atagaaaatt ataatagggt taaagtacag gaagaatata
     4081 tagacataga atatgatgaa gaagaagagg aagaagaagt ttttgaagta aatagagaag
     4141 aagataggtt tgtttcctca aaacctacta aatcaaagaa agttcaaaaa gatcagaata
     4201 atattgaaaa aataaaaaga gctaatggag taagtcaagg agagagtgga aataaacaaa
     4261 ccagtacatt acttatgcca agacaaataa aaaaaggttt gaaattattt aaagaagtaa
     4321 aaccttttgt aacagattat atagataaga caagatggtg gaaaatagag ataaatccta
     4381 caacactgtg tggatataca atgccttatt taggatattt aaacgtattg aattacacta
     4441 tgtatagtga tgcagtttta cattcatata aatacagaca ctatttattt ggagttcagt
     4501 atgatgaata taataagaga aaacattata tttatgctat cccaggaaat agaaatgaac
     4561 agccagacaa tggaagcaca ggatttttaa gatatcaagc ttgtgataat agaacaaata
     4621 gtttaggata ttggctatgt tttatagatt ctagacaaag aaatatagtt aggtaaaaat
     4681 aataaaaatg aaaaaattta gactcaattc ttattttact gaaaaaatta atgaaatgta
     4741 gtacaaaact ttaaaaacta ttgataatat ttaaatatta cactacaata tatcttgaaa
     4801 tacctattat attatggagg aaaattatga taaacgagca aagaatttta aatgagtttc
     4861 ttgaattagt acagatagat agtttatctt taaaagaggg gaatgttgct aaagtattag
     4921 ttaaaaaatt agaagaaata ggttgttcag tagtaataga caatgctggt gaaaaagcaa
     4981 atggagaaac tggtaatata atagctacat taaaaggaaa taaagaagga aagaaaatat
     5041 tatttagttc acatatggat acagtaactc caggtatagg ggtaaaacct atagtggatg
     5101 aagcaaatgg aataataaaa agtgatggta ctacaatatt aggttctgat gataaagcag
     5161 gtatagcagc tatattagaa ggcttaagat atataaatga aaataacata gaacatactg
     5221 atatacaagt tgtattctct atatgtgagg aatgtggatt agttggagct aaaaatttag
     5281 actatggaaa aatagattct gaatatgcat ttatattaga tagtggtggt tctccaggtg
     5341 aaataatagt aaaagctcct gcacaagatg taataaatgt taagatatta ggaaagactg
     5401 ctcatgcagg tcttgaacct gaagctggaa taagtgctat aatggtagct gccagagcaa
     5461 tagaaaatat gaatttactt agaatagacg aagaaacaac tgctaacata ggaattataa
     5521 acggtggaac agctacaaat atagtaacag gagaagttaa tatagttgca gaagccagaa
     5581 gtctaaaaga aaataaatta gatgttcaaa ctaaacacat ggtagagact tttgaaaaag
     5641 ctgcgaaaga ttttggagct caaattgaga tagatgttaa tagagcatac acaccaatag
     5701 atgttagtga ggattctgaa ataataaaac ttgcaaagaa ggctttttca aacttaggta
     5761 tagaaggtca tactgaatct acaggaggag gtagtgatac aaatatatta agtaagaatg
     5821 gtatcgaagc tataacttta ggaattggta tgaaaaatgc tcatacacta agtgaacata
     5881 tagctataaa agacttatat gattctgcta aaatggtagt ggagataata aaggaagcat
     5941 aaatttaaat aatagtgtat aatatatata tcgcctagct taagctaggt gatttttcat
     6001 tttatagaag attgataatc tctattgaaa gcaaagagta tacatttgca tataagagag
     6061 tgtttaattt atgttcaaat gtaggttctt aataatattt agattgtttc ataagggagg
     6121 aataaatatg gaaacaaggt atgaagaagc aaataaaata acaatacaat caatactttg
     6181 gaatatagta ttaacaataa ttaaggttat agctggtgtt attggtaatt ctagcgctat
     6241 gattgcggat ggattacatt cagcatcaga tattataagt tctataggag tactaatagg
     6301 caattatgta tcttctagac ctggagatag agagcataat tatggtcatg agaaagcaga
     6361 gacattagta tcatttgtgt tatcaatatt gcttattttc gtttctataa caataggtat
     6421 agaggcaatt aaatctttat ttaacttaga tgcattaagt gttccatcaa tattaccatt
     6481 agtagtatca gttatttcaa tcctgataaa agagtatcaa tatagaataa caataaaagt
     6541 tgctaaaaag ataaattcac cagccctaaa agctgatgct tggcatcaca ggtcagatgc
     6601 tttatcttca gtagctgctt ttataggtat aggtggttct attttagggt ttaaaccatt
     6661 agacccaatt gcgtcagttg tagtggctat atttgtagct aaagtaggaa ttagtatact
     6721 tataagttct gttaatgaat tgatggatgt ttcagttgat gaagaagaga taaaagaatt
     6781 aaaatttatt gtagcagata cagaaggtgt taaaaatctt ggtgatataa agactagaaa
     6841 acatggagct atggcatatg tagatttgac tatatgtgtg gatgaaaatt tgacagttaa
     6901 gcaaggtcat gatattgcta ccaagcttga aaaacatata ataaaacata tggaatttgt
     6961 aaaaggtata acagttcacg ttgaaccatg tactaattgc caaggaaata agtgtaataa
     7021 ttaattaaaa cttaaaaaaa agtgtaaaaa gttatataat tgttttatga ttataaactc
     7081 taggggtggt taaatgagca agaaaagaaa actaaagaaa aaagctgcaa tattaatagg
     7141 agtagttgtt ttctttataa tatactttat atcctttaga ataggaataa atatggaaaa
     7201 ggataaaagt gaagtgccaa agaaggtaga aaaagaagaa gaagataata ggtctttaag
     7261 gactcaaata aaagagaaga agaaggttta tatatcagat aaaaatatag aaaacataaa
     7321 aattgaagaa gatatgtggg atgaaataaa gttatttttc tcagatttta agaaagtaag
     7381 aaatactact tcttcttacg aagcagttta tgaaggttat tctgatgatg gagttaaatt
     7441 ttctactgac cttaactttt ttagagtata tactgtaaat aaagaagaat attataaagt
     7501 accagttagt tctaaggatg cttttaagaa acttttagat gagagtatat atacttcgtt
     7561 tgacctttta agtcattata aagattggaa agaggttact ataactcatg gaaaagaaat
     7621 taaaaaagtt catagatgga aatttgatga tttagcacat aagatgattt caaagagggt
     7681 tgtaggaaaa gttcaacctg agaaaagtaa ggaaagaagt gactataatt ttactataaa
     7741 tataaaaggt gataattttg atgtaaaagt tgatacaatg ggaaaagatt atgtaaaagt
     7801 tacttctaag aaaaatgtat cttactatga ggttcataca agtttatttg attaccttaa
     7861 aaatgagata tttgaaattg gaaagaaata agactttaag aaggctatca aattagatat
     7921 ttgatagcct aaattatttt atatattatt tttcaaccaa caagtctgca accttataaa
     7981 ttggaccagc accagcagtt attactaaat cattatcctt aacattttca cgtaagtatt
     8041 ttactatatc ttcaaattct tttatataga tggcatctac attgttttga tataatttct
     8101 caaccaagtc cttagaatgt atatctcctg ggtctttttc cctagcagca tatatatctg
     8161 ttatgataac tttatcagca gcatagaatg cctctgaaaa ttcacctaaa agagattttg
     8221 ttctagtata agtatgtggc tggaagatac accataaagt tgattttttt aattttttgg
     8281 cagcagctaa agttgctttt aactcagttg gatgatgtgc ataatcatct actactaaag
     8341 cattcttata gtacccttta gtttcaaatc ttcttccgac acctttataa atttttatat
     8401 tttttcttat agtctctaag tcaataccag aaacaagagc tgccattatg gcagaagaag
     8461 cattgtatat gttatgaaga ccatatacag aaagttcaaa ttctcctaaa tctcttcctt
     8521 catattctat tctaaatatt ccatgaccac tattgtcaaa gtgaatatct tttattacag
     8581 catcattgtc agaatctctg ccgtatttta taatagtagc tttaacatca tacaatatgt
     8641 catcaatatt ttcatcatcg ccatttatta tgaaatagcc ttctgggggt aataattttc
     8701 caaatttgtt aaaagaagcc ttgatttcat ctattccaga gaaataatca agatggtctt
     8761 cttcaacatt taaaactata gatatctttg ggttaaaatt aaggaaacta tctacatatt
     8821 cacatgcttc agttataaag tggttggaat tacctatctt aacattacca cctatcatac
     8881 ttaagttacc accaactagt atagttgggt ctaagtctgc atattcaaag attgtagata
     8941 acatagatgt tgtagaagtt tttccatgtg ttccagaaac tgctatagaa tttttatatt
     9001 ccctcatgat ttggcctaaa aaagttgctc tgttcattac caatttattt ttttctttag
     9061 cagccacaag ttcttcattg tctggatgaa ctgctgctgt ataaacaacc atatttacat
     9121 catctgatat attatctttt ttctgaccaa tataaatagt tgccccttgg tccctaagtt
     9181 tgtctaatag gtatgattcg tttgaatccg aacctgaaac ttgataacct ttattgatac
     9241 atatttcagc taatgcactc atgctgatac cgccaattcc tataaagtgt atattcataa
     9301 taaaaccacc tttcttttta attaagtcct acaatatgaa ctatttctgt ggtataataa
     9361 tataatgttc aatattttat aattaatatg catataatat tatatgtatt ttatttttga
     9421 atataataaa acataagtaa tattatacca caataagaat tgttatgcat tagttagttt
     9481 aacgttatat aaaggtactt gtaggaggaa ataataatga aatttaaaag aacagagaga
     9541 attggagcaa tagttaaaat actgtctgat aatccaaata aaatatatac tttaagttat
     9601 tttacaaatc aatttaatgc agcaaaatca acaataagtg aagatttatt agttgtaaaa
     9661 aatgtatttg agaagctaca tttaggtaaa gttataacta tttcaggagc agcaggagga
     9721 gtaaaatata tacctaaaac ttctatagca gaaaatgcag agttcttaat ggaattatgc
     9781 gagagaatat gtgacaagtc tagaatacta tctggtggtt ttttatactt gattgaccta
     9841 atttacgacc ctacaatagc tgctaagata ggaaagatat tcgcatcaaa tattgagtat
     9901 gtagatgcag attatgttgt gacaatggaa acaaaaggta tacctatggc acttatgact
     9961 gcaaaagcta tgaatttacc tctagttata ataagaaaag atataaaggt atcagaaggt
    10021 cctacactta gcatgacata tgtgagtgga aacagttcta aagttgaaag tatgagtttg
    10081 ccaagaaaag ctcttaaacc agatagtaaa gttataatta tagatgattt catgaggggt
    10141 ggaggtacta taaaaggaat ggtagacctt atgaatgaat ttggtgcaga ggttataggt
    10201 actggagtat tcatatctac aacgaatcct tcagaaaaaa tggtaaaaga ttatatttca
    10261 ttgattcaat tggatgttaa tggagataaa atagtagtag aaccaaactt aaagactttt
    10321 aaagatgaat atagaaatga agatttagac gaagaagaag attcagaatt tgaatttgag
    10381 atagatgaag attaataaaa aaaatattta gaaaaatatg taaaaataaa aagaggatat
    10441 ccctagttgt tcatagaatt atttaaaaaa cagtttatat taatttattc cagcgataat
    10501 aattttaata taaattaatt tctgtcaaaa tcaaaggggg atattttggt atgaagataa
    10561 ctgacgtaag agtaagaaaa ttaacagaag aaggtaagat gaaatgtata gtttcaataa
    10621 ctttcgataa tttgtttgta gtgcatgata taaaagttat agaaggccat aatgggctat
    10681 ttatagcaat gcctagcaga aaagtaggcg aaggaaattt cagagatata gctcatccaa
    10741 taaatgcaga aatgagacaa gttttagaag atgcagtttt acaagcgtat catgaagcat
    10801 tagttcaatg ggaagtagct gctgaataat aataaatata taaaatggag agagcccgaa
    10861 catttaggct ctttttaaat gtagaatttc tattaaaata tgtacttttt agtaaaaaag
    10921 tgctatatta ttagtgataa tgtatatgtt gtatacaaat tgttagataa atgtattata
    10981 atgtatattg attaagagaa taatacgaat aaaattaaat tagattgaat ggagtgtatc
    11041 aatgaacttt aaagctataa tccttgctgc tggcaaagga acaagaatga agtctaaata
    11101 cccaaaagtt atacataaag tatgtggaaa agaaatggtt aaccatatta tagatgtttc
    11161 aaaaaaatca ggggtaaaag atactgtcgt gattttagga catgaagctg atgtagttaa
    11221 agaaaagctt gcagaagaaa ttataatagc catgcaaaca gaacaacttg ggacaggaca
    11281 tgctgtaaag atggctaaag aatatattaa tgatgaagat acaatagttg tactttgtgg
    11341 agatactcca cttataaaag aagagacact aaagagacta tttgaatatc atatagaaaa
    11401 taaatatcat gctacagttc ttacaacaag agttggaaat ccaacaggtt atggtagaat
    11461 aataagagat aagaagggtg accttcttaa aatagtggaa caaaaagatg caaattcaga
    11521 agaaaagatg ataagtgaga taaactctgg aatttattgt tttaatggaa agagtcttag
    11581 agaagcacta gatttgctaa ataataacaa ttcacaaggt gaatattatt taactgatac
    11641 tgctaaaata atgagagata aaggattaaa agttggtgca tttgcaggtt caactattga
    11701 agaacttatg ggcgttaatt caagagtaga attatcaaag gcagaagaaa ttatgagacg
    11761 aagaataaat gaatctcata tggtaaatgg agtaactata atagacacta actctaccta
    11821 tatagaatct gatgttatga taggcaatga tactataata tatccaggtg ttatgttaca
    11881 aggaaaaaca aggataggtt cagattgtat aattggaatg aattcatcta taacaaattc
    11941 agaaataggt gatggtacag aaataaaaaa ttcaactata attgatagta aggttggaga
    12001 aaattctact gttgggccat atgcatactt aagaccaaag agtgacttag gaaataatgt
    12061 aaaaataggc gattttgtag aagtaaaaaa tgctattata gaagatggct caaaagcatc
    12121 tcacctttct tatataggag atgcacatgt cggtaaaaat gtaaatattg gatgtggagt
    12181 agtatttgta aactatgatg gtaaaaataa atttaaatca atagtaaaag acaatgcgtt
    12241 tataggttca aattctaatt tagttgcacc agtagttgta gaagaaaaag gatatatagc
    12301 aacaggttca acaataacac atgatgtccc agatggtgct ttggcaatag caagagaaag
    12361 acaagttatc aaagaaggat gggtagaaaa aaagaatcaa aaagacgacc aaagtaagta
    12421 attatgtagt ttattatttt ggctaaaaaa tttagctcga aattattata aattatattc
    12481 attagtattt taaaattttc aggagggtta cataaatgaa tactagcgga agcgagatta
    12541 aaattattgc aggtaattca tcgaaagagt tagcacaaaa aatagctgac tatataggtg
    12601 tatcagtact agattgtgaa gtaggtacat ttagtgatgg tgaaatatgt gtaaacatga
    12661 atgagacagt aagaggttgt gatgtgtttg tagtgcaatc tactaacagc ccagtaaatg
    12721 ataacttaat ggagttatta atcttaattg atgcgttaaa gagagcatca gcaggtagaa
    12781 taacagcagt tattccttac tatggatatg caagacaaga cagaaaggct aaggcaagag
    12841 atccaatcac tgctaaatta gttgcaaatt taataactgc tgctggagca gatagagtat
    12901 taacaatgga tttacatgca gctcaaatac aaggatattt tgatatacca ttagaccact
    12961 tactaggtgg aacaatatta gcaaattatt ttaatgaaaa gaaaatagaa gatttagtag
    13021 tagtatctcc tgacttagga agtgttacta gatcaagaaa atttgctaat actttaaatg
    13081 gagaagttcc aatagctata atagataaaa gaagaccgaa agctaatgta tgtgaagtta
    13141 tgaacttaat aggtgatgtt aaaggtaaaa atgttatact attagacgat atgatagata
    13201 cagcaggaac aatagttaat gctgcaaatg cacttaaaga gtttggagca aaagatgttt
    13261 atgcttgttg tactcatggt gtgttatcag gtccagctat tgaaagaatt gctaattcag
    13321 aaataagtga gttaatagtt cttgatacta tacaacttcc tgaagagaaa agaatagata
    13381 agataaagat aaaaacagta gctcctttat ttggagatgc tataagaatg atattctcta
    13441 atgaatcagt aagtaaatta ttctaattta ttgtatttat gagagttgtt taaaaggata
    13501 taaacctttt gagcaactct tttttgattt tatacttatg gaatgttatt tttttcttgt
    13561 tagaaaggta tatattattg taaaaagaga ttatagttta aaattatgta tatttaagta
    13621 aaataaacct aataaagggt atattattca aaatattatg aatataaaaa atattacaaa
    13681 aaagtattga aaaaattcag attatgtagt attatgtaaa tataaataaa gaaagaggtg
    13741 tttccaatgg gaaggttaga agatgaaccc ctaataatta aatgattcag tcacaatcta
    13801 aagtgaatat aaaaatctat tgaaagttta tttataagaa ttgaaaatag tatctattct
    13861 atctttagtg tttgtgagag ggttctggat tatataagat tactgtaaag agtaattttg
    13921 cacaataagc atatctgtaa atgaagagtt tcgtttatat aatgattgtg gctgaatcat
    13981 aactataata gatagaatgt ttataataga tgattttata aaatactata ttgtaatagc
    14041 ttttagcttt caaataaata ttagcaaagg atggaaatga caatgataaa actaaatgat
    14101 aatttataaa tatctcctta cgatatataa taattgtagg gagatgtttc tttttttgta
    14161 tagcataatt tttctagaaa ttctgtttca atatgtaaaa agttggtata tataaatagt
    14221 ataaacagtg ttgctaaaat atggtagata ttatataata taagtggata tgtatataca
    14281 ttatgagtta tataatttga atttaataaa atagaaacgt tgatttatgt tctgtaatgt
    14341 gggcaccttg gagcatattg agttagtggt gcaaccggct atgaaattgt atttatttat
    14401 aaatactatt attttcatag ctttttttat ttaaaaaatg gttatatgtt agaaataaat
    14461 ttggttagta cgtttaaata attttactta gatttttaac cagatatatt gtaagtaagt
    14521 attaattaaa aaatttaagg gggaataaaa atgaagttaa aaaagaataa aaaaggtttc
    14581 actttagtgg aattattggt agtaattgca attataggta tattagcagt agtggcagtt
    14641 ccagctttat ttagtaatat aaacaaggct aaggtagcaa gtgttgagtc tgattatagt
    14701 tcagttaaga gtgcagcatt atcttattat tcagatacta ataagatacc agttacacca
    14761 gatggtcaaa ctggtttaag tgttttagag acttatatgg agtctctgcc tgataaagct
    14821 gatataggtg gaaaatataa attgattaaa gttggtaata aattggtatt acagataggt
    14881 acaaatactg aaggagttac attaacagag gcacaatcag caaagttatt gagtgatata
    14941 ggtgaaaata aaatatatac aagcgctaca acaaatagtt tgggaaatcc attaacaagt
    15001 aatacaaaaa tagataataa cgttctatat atagtactta tagataatac tgtgatggac
    15061 acaacaaaat agttaaaaca atatttttat aaaaatattg taaatttaat gaaaaatata
    15121 atataaaaga caggaagaaa atcttcctgt ttttttatac tattttcttt aatgttcaat
    15181 tatgaatgat gatataattg agatagtaaa ggagtgaaac ctgtggctaa aaaagttaga
    15241 ataggagaca aactggtcga aaaaggatat ataacagaag aacagcttaa atgggcacta
    15301 tcagaacaaa aaaacagtgg aaaaagacta ggagaatttc tagtacaaga agggcttata
    15361 gatagtaact tactaataag tgtattaaaa gaactcttgg atatagaaag tatattttta
    15421 gaagggacag aaatagacac tttagctaca aaaatggttc cagaaaatat atgcaaaaga
    15481 tatactgtat ttccatttaa aattgatgga aataaaatat gtctagccat gtcagaccca
    15541 caagatagag aagcagttca agatgtaagg cgtatgtctg gtaaagacgt agagatattt
    15601 atatcaagta cagaagatat aaacaaagca ataggtcatg cttatgcaca ttcagaaata
    15661 aataaggcaa tgactgagta caacaaaaat agaactggtg gagtaagaga aacagtaata
    15721 cttgaagaag atgtaaatgc tgctccaata gtacgattag taaataacat actagaaaat
    15781 gctgttagaa tggaagcaag tgatatacat atagaacaaa gtgaaaatta catgcgtgtt
    15841 aggtttagga tagatggaat gttaagagaa tatatgagga tgaactcagc accatataag
    15901 gcagtaatca gccgtatcaa gataatgtca gatataaata tatctgaaaa gagaatacca
    15961 caggatggta gaatctattt aaaagtagat aataaaccca tagattttag ggtttctaca
    16021 atgccaacta acagggatga aaagatagct atgagagttc ttgataagag taattttatg
    16081 gttagtaaag aagtactagg aatagacgaa catggctcta agatatatga tgaattgata
    16141 aacactcctt atggtcttat attggttgtt ggccctactg gaagtggtaa aaccactaca
    16201 ttgtattcaa tgttaaatca actaaataca gaaaatagaa atctattaac aatagaagac
    16261 cctatagagt atgaattacc tggtgtaaat caatctcaaa taaatgaaaa ggcagggctt
    16321 actttcgcca gcggacttag agcttttatg cgtcaagacc cagatataat aatggttgga
    16381 gagataagag atactgagac agcagaaata gcaattagag cttctcttac aggacattta
    16441 gttttaagta cattacatgc aaatacagca gttggagcaa tttcacgtct tttagatatg
    16501 gatgtggaaa gttttttaat aacttcatct gttttaggtg taatatctca aagacttaca
    16561 cgaaaaatat gtgagcattg taaggtttca tatgaagcag atattggaga aaaaaaggct
    16621 cttggtattg atgtaaatga atcagtaact atttatagag gaaaaggttg tgaaaggtgt
    16681 aataatactg gatataaagg acgtctaggt atatttgaaa tgcttgaaat tactccagaa
    16741 ataaaagagt taatagattc atctgcaaat caaagagaaa ttttaaagat ggctagaaaa
    16801 caaggtatgg ttagcttaaa agaggatata gttaaaaaag ttttaaatgg aaagacaaca
    16861 gtagaagaaa tgattagaat aattttgatg actgattaaa ggatgatatt atgaaaagtt
    16921 ttaaatacac agtaataaaa gaggacggaa aaaagcaaac tgatgtgatt gaagccaacg
    16981 attttaatga agctagaaat cttcttagaa agagaaatct tagagttatt gaaatcaaag
    17041 aaagcaaaaa taaaaatata agattatcta gtagaaagag aaaaaaagac ttaggagctg
    17101 accagataag ccacttttgt agacagtttg ctattatagt atcttctggt ataaatagta
    17161 tttctggatt agaaacatta gctagaaggt caactaacat tacattaaga gaagaaatta
    17221 atcgtatagt ggcagaaata aaagtaggtt ctactattgc agattcaatg ctttcatcaa
    17281 aatcaaagtt tccaaagtta cttggtgcta tggtagctac aggtgaagct acaggtaaat
    17341 tagatgaagt attaaaaagt atggcttcat tttatgcaag tgaacacagg gttaaacaaa
    17401 agcttagaaa tgctgcaaca tatccattaa tagttttgat ttcttcattt ataatgattt
    17461 ttatatttac aacatttatg gtgcctaaga tgattaattc aattacaaca gtaggagcaa
    17521 ctcttccact tataacaaga attgttatgg ggtttggtat gtttatgaag aagttttgga
    17581 taatagtact tttagttatt atattattta tctatcagtt taaaaaatat ctaaaaacgt
    17641 cagttggaag ggctcataaa gatagagtta taaataagat acctgtttta ggaaaaggaa
    17701 taaattgtat ggtagctaca cgattttcaa gagcattgta tctatttgta tcaacaggat
    17761 acccattagt tcaagggctt gattatatta tagatagtgt aaataacacc atggcagaga
    17821 agattttagc atcagctaaa gatggaataa caagaggaga aggcttagca gaaaatttag
    17881 agagatatac gtattttgac tctgtgttgg tacagatgat agctataggg gagcagacag
    17941 gggaattaga gaatataagt agacagatgg cagaatttta tgagtatgaa tccgagattt
    18001 atttaaatag gatggcctca atgatagagc ctgttttaat aattgcagtt ggtattatag
    18061 ttgcgatact ggttgtatca atattcatgc caatgcttag tatatatgat gccatgtagg
    18121 agggaaagga caaatgaaca aggtttatgt atcatattat ggcgattcta tttccatagt
    18181 tgagggcaga tataaacacg ataaggataa gttttatgta aaaaatttta atgtgttttc
    18241 aatagaggat attgcacaag atttttctaa ggaaaagtat gctctattga gatacgcatt
    18301 agaaaatgtt aagttaaaat caagacatgt agttttttgt ttaaatacaa gagatgtaat
    18361 tttaaaaccc caaaaacttc ctaatatgga caaaaaggat ttagacggat ttatgaagct
    18421 agagatggac gaaatgatgg ctttggagtc agatgaatat gtattttcgt atgaagtatc
    18481 tagtgaaaca cctgataatt cagatggcga tgaatcaagt ttaaatctta ttattggagc
    18541 tatacaaaag gaagaggtaa atgcaattgt agagataatg catgaatttg atttagtatt
    18601 agatagaata gatacattat caacagctta cttaagaatg cttaaaaatt tagactacaa
    18661 tgatataatg gtaaccaata taagtgacaa tagcacaatt ataaatatat ataagaaaga
    18721 tactttattt attagtgata atatacctat aaagataact gctaaagata tggacatgca
    18781 attattaaat gtagtagaag aatcaaaagg tcttatgaat tactattcgt caagaaactt
    18841 tggtaaggtt acggatacta tattaatatt aggaaaaaga tattccaata aattaattta
    18901 tgaaactttt agctctgcat tttcaagcta tgtttctcaa ggccttactg agataatgga
    18961 tatatctgat atgataactg gagatataga tgaagatgat ataaattcaa ttgtagagct
    19021 tttaggaagt atgatagaac ctaaagggaa aagagatttt gaaaatataa actttttacc
    19081 aatggatata cttagaaaac agataaaagc taaacaaatg aaaaacatac tgaaaattgc
    19141 tccaatagtt attttgattc ttgctatacc ttatttatgt ttaatttttt caaataattt
    19201 agcaaataag gaacttgaaa atgttaatgg tgaaataaga aatattgaat ctgcatatta
    19261 tcagattgga aatatagaaa aaatgataca gagtaaaagc gaagaaataa aggtatatga
    19321 tatgttaatt gggaaaaagg taaagtggga aaatttattg gatgatatag ataaaaatac
    19381 tccaagtaaa gttgaactaa caagtttatc aacaacttat caaaatcttg gaactaataa
    19441 taaagctgat aattcaaaaa gtaataagac tacaacagat aaatcaagta acaatgaaac
    19501 aaaatcaagt acgaaaagtg atagtaatgg agataaaaca gataatgatt taaatgaaga
    19561 taagactaca aatgtgtcag aagcattaac tggaaatagt gagactaata gtaataacac
    19621 agataaagat gataaaactc cattgtatga taagatacca aatgttataa atatagaagg
    19681 aaatgctaaa gattcctctt atgtaggtca atttttatat aaattaaagg gattatacta
    19741 tttcaaggat gttaaattac atagcataaa aagtaatgca gaagattcta actatacatt
    19801 tagtatgaca ttatacctga aagaaggtgt attaactaat gaataaagat atatttaaaa
    19861 aagatataaa agatatattt aaaatggaag ttagtctaaa agggattctt ataaaggtta
    19921 taattttatt agctatttta tcatatggtt attttggact ttaccctcaa tatcagaaat
    19981 atattgctac aaaaaattct gtaaaacagg ctactgaaaa attaaatata tataaagaga
    20041 aaactgcact tatgccaaaa tatgaaaaac aacttgaaga agttgaaagt gaattaaaag
    20101 aaaaaagcaa aaatctttta tatgatatgc aagatggtct ttttttaata ggtttagata
    20161 aaaaaatgaa ggcttataat attgacttaa ccagttatga cgttggaaat gttaaagaat
    20221 acgaacattt ttatggtata tcaacaacaa taagtgttag aggtgattat agaaatgtca
    20281 aaaaattaat ggcttattta gaagaacaaa aaaatgttac tcaaatacta gattatacaa
    20341 tgacttcata tatagaacca gaaaaagaag aggaagaaac aacaaacaat aaggatttaa
    20401 ttaaaaatgt gaacataaat aaagatacaa aaatatactg ggtggaaggc gaaaataagt
    20461 ggcatttata taaggattgt ccattgataa aagagggaga gattctaaaa aatgacaaac
    20521 ttttagatag gtctactaat tatctatgta atacttgtgt acttagagct gcaacagaaa
    20581 aagaagcaaa aaaagaagtc gtagctccaa aggcagaagg tgtagtagat gctacattta
    20641 aatttataat gtacactaaa gagaatacta caatgatact tgatacagat aatccatctt
    20701 catggagacc aggaaaatat aatccattta agtctagtgc gaaataaagc taagtaagct
    20761 ttaaagatag gtgatgagtt tgtacaaaaa taatgaaaaa ggtcttactt tattagaagt
    20821 aataatagct gtttttatac tgacaatagt tttaagtatt tcttataaag tgttcaatgg
    20881 tataacatca gcagtaaaaa agcaacagat tattacagat gctcaagtaa atattaatct
    20941 aattaataag tatctaaata gagatttgga aaactgtaaa gaactaacta aaactggttc
    21001 aggtaataat tatgaataca atatagagat gccagataat gttgtaaaat atgaagtttc
    21061 tatagaaact aaaaaaaata cagaggtata ttctgtaacg agaatccaaa acaatacaat
    21121 tgatacagaa aatgaagtta gagaagagat aatctataat caaccactag ttcaaaacaa
    21181 taaagaaatg aaggaaacac catttaaaat agaaaagcaa actggtaaat ctatttatac
    21241 tgtaagtata tactataatg aatcagtgca agaaagtcat aaaaatagca atttaaataa
    21301 caaaacttat acatttgatg ttatgtcaag aataggataa taagaattta ttaaaaaata
    21361 agtaggttga taaaatatga gtaaaaagaa tagtgaaaga ggattttcct taattgaagt
    21421 gttagtagct atggctatta tgggaattgt actatttgca ttttttaata ttatcaatac
    21481 taataacaaa gcaaacacta aaaatgacac agatataact tccttaaact atgtccaaag
    21541 tgaaatagaa aatctaagag aaaagataaa aagtggagaa tttgattttg atagtttaga
    21601 taaactagaa gatggaactg tcgtatatga aaaattaata gataaatcaa aaaaagttgt
    21661 atatgataaa gttcttagtg aaggtgatgt gagcttatat gatactccat atgaaaagat
    21721 tacaacaata aaagatgaag atggtaattt aatcgacaaa ggaaatatta caaataagat
    21781 aaagactatt gtagaagata aaagtggtca gatatataaa atagctgtaa ctgggaaaag
    21841 catgaatgac tattcaagta aaaaagaggt taaaattgta actgaaatat ttaaagataa
    21901 ataattttac ttgggggtga gattttgagg aagtggaata aatttaaaag tgaaagaggg
    21961 gcagctttag ttttagtttt ggttgtagta gcattgcttt ctattgttgg attgatattt
    22021 tcaaatcaga tagctaatag aataaaatct acaaagacta ctaatgaagg tatacaagca
    22081 aagtatttag cagagacatg tgtggaaaat agtattgata aagcatatga aaaattgtat
    22141 gatgagcttg agaaaatgga taatgagttt aaaagtgaaa atcaagaaaa atccatatct
    22201 aggtcaaaat taagagatat atcagatgaa gattttaata atcaagatga ggaaaatatt
    22261 gaagcagaaa gacttggata tatgaataat ataaatttct atttaaataa agcttcttca
    22321 gacttggaaa aagctagtat ggaattgaaa aagctatatg atttatatat gttggactat
    22381 agagacattg aatatgtaga tgcaaatatt atttcacata gagattctat cttagaaatt
    22441 tgcaagaatt atacttctgg agatattagt aaaataaatg aatatattct gaaagaagat
    22501 atagactcaa ctacattgat tgaggcaaag ttagtgaata atgatattct tttaaaaatg
    22561 tttttagaag aaaataaaat tgaaaatgaa catttaaatt cagccttttc acatacttat
    22621 aaggcattag ataacatttc tttggcgatg caaaatatga ttgagtatag acatacgttt
    22681 catatagatg agccaaaagt agaagtatct aatgggattc cagattctca acaatattat
    22741 gaactaatac aaaatcctat aataaatagt atggagtata tctggaatag taaatgggat
    22801 acattagaaa aactattgga aattttacca aatcaaactc aaggatttaa ttctttaaga
    22861 gttcatttga gaaataatgt aagaaaattt gagaagttaa gcgataatat atctagtggt
    22921 aaaaaaaata cagcaaaaaa tttcttaaag tataaagagt tattgtatga aatatcagac
    22981 caatgtaatc aactaaaaag tatgtcttat gagaaaatac ctgttaaata tgataacatg
    23041 gctttgataa caacttttga ctatatacaa aatgagcttt tagcagagat aaaatgcaga
    23101 ttaaaagaat taaaaccaca agaaattgat aaaacagaag gtattactat aaaaatacct
    23161 ttttataagg ctgactacga catgacaaaa gaaggatggc ctaaattaaa agaaaatggt
    23221 tcaggggcgg agttaagctt aatggtaact ggtgataaag atggaataaa agaagtagaa
    23281 gttacagatg gtaaaaaaaa tattattggt ttaggtgttg aagaaaattc aaattcaaag
    23341 tataaggttg acgccattgt aaactttaat ctaaatatag atacaaatgt agttggaaat
    23401 tatgatataa aagataaaat tttgattaat catgatataa gttcatacaa aaaagtaaac
    23461 taaatataag aagcaactta gtataaataa ataataaaat aattttatta tttactacta
    23521 aaaaaataag taaactaata gaagatatgg agagtaaata ttatgtatat ttatgattta
    23581 ttagagcaag gtataagact aaacgcatca gatatacaca taacagttgg aacaaatcct
    23641 gtagctagag taaaaggtgg atttgtaaaa ctaagtgaac aaatattgac atcagaagta
    23701 acaatgcaaa tggcaaaaga catagcaggg gaatctatgt ttaaagtaat agaagaacat
    23761 ggagaagctg atttttcagc ttctttaaaa acaggagaaa gatttagggt aaatgcatat
    23821 aggcaaaaag gaaattacgc aatagcaata aggactataa cagcagaaat tccaacattt
    23881 gaaaaattag gccttccaga gagtataaaa tcttttactg aaaagcataa aggattagtt
    23941 ttagttacag gtccaacagg aagtggtaaa agtacaacac ttgcaagtat gataaatata
    24001 ataaatgaaa aacaacaaaa acatataatt acacttgaag acccgataga atatgtacat
    24061 catcataaac aaagtttagt aaatcaaaga gaagtaggaa ctgatacaga aagttttcat
    24121 tcagccctta gagctatttt gcgtcaggac ccagatgtaa tactaatagg agagatgaga
    24181 gaccctgaaa ctgtttcaat agcattaaca gcagcagaaa caggtcattt agtattttca
    24241 acactacata cagtgggggc agctaagaca attgatagaa tagtagacat gtttcaacca
    24301 tcccaacagc aacaaataaa aacacagtta tctacagttt gtgaaggtgt ggtatcacag
    24361 cagttacttc ctacagcaga tgggaaaggc agaatagcag caattgaatt gatgtttgct
    24421 actccagcaa taaaaaacct gataagggaa ggcaaaacat atcaaattcc aaacatgata
    24481 caaactggag ttaaagtagg tatgaaaact atggaccaag atttaatgga actttataaa
    24541 aatggaaaga ttacaaaaga tatggcactt agtaggtgta ctgaccaaga gtttatgaca
    24601 agaatgatag gtggagtaaa ctacaatgga tattataatc gttaatctat atgtatttat
    24661 ggtgggtata gtgtttggaa gtttctttaa cgtctgtatt tttaggattc cagaaggtat
    24721 atctatttct agacctcctt ctcattgtat gagttgtaat actagactaa agccaataga
    24781 cttaatacca gtgttaagct atttaatgag tggcagaaag tgtagatatt gttatgagaa
    24841 aatttctagt agatatgcta tagtggaatt aattacagga cttttatttt taacagtatt
    24901 tatgttatat ggaatatcta catcaactat ttattatttg gttcttgtgt ctttactgat
    24961 tataattaca tttatagata ttgaccactt tataatacca gataaaatat tggtttttgg
    25021 agctatattt tctatgattt ttaacctttt atttaaagaa attccagtta aggattcaat
    25081 tctaggtgtt tttatttgtg gaggaagtgt ctggattata gttcttttga ttgaatttgt
    25141 aataaaaaaa gagtgtatgg gtggaggaga tatcaaacta tttgcaatgc taggttttta
    25201 catgggtgtt aaaaatggtc ttttgacagc cttactaagc gtttatgtgg gagcagtcta
    25261 tggaatctgt gtaataatta taagtagaat aaaaggaaag gaatataatt ctgttatacc
    25321 atatggacct tttatatcta ttggagcatt aataacaatc ttatgtggaa agcaattact
    25381 tgaactttat ttttctatgg ttatatagat gatagtcaag ttaaataaag ttttaattta
    25441 aaaaaatgaa ttaaagtatt taataaggac aatattatta ttaaccctat tactaaagtg
    25501 ctacaaacta tggagtatat aataataatt tccttatttt ttgcacattt gataaataaa
    25561 tctctatgtg ttttttgtga tagagaaaag aaaaatttat acaatacgtt ttttatttac
    25621 ataataactg tagttttatc tgtattaata tatctaaaat ttgggattag tgtagaatgt
    25681 gtacaatata tggttttaat tccattttta ataattatat ccattgtaga ttactacaca
    25741 atatatgtat atgatataac aatagttagt ggtataatag ttcaaggtat aatttttttg
    25801 gtctttttca atatagttca atttgattat ataaatcata tatttggact attgataggc
    25861 tttattatac cttatattat tgctaaacta acaaaaggat taggctatgg agatgtaggt
    25921 ctctatagct tatgttgctt tgcattggga aataattatg ggttttatgt gatagtttta
    25981 tcatttattt tagcgttttt gttttttatt attttataca taactaaaat atttaaagat
    26041 gtagatataa gaaagcaaat tccttttaca ccttttattt ccttgtcaac aattttaata
    26101 atatttacag agtataatat atttaatttt tattataatt tgttatacaa atttatataa
    26161 caggaggtaa gaaatgtacg tagtagtagg gttaggtaat ccaggaaaga agtatgagaa
    26221 gactagacat aacgttggat ttgatgttat agatattctt gctaaagaat acaatattag
    26281 tgttactaaa ataaagcata aagctttgat tggtgaaggg agagtaggta ctgaaaaagt
    26341 attattagtt aaaccacaaa cttatatgaa tttaagtgga gaaacattaa tagatatata
    26401 taaatattat aaggttgatt taagtaatat agtagtagta tatgatgata tagatttaga
    26461 agttggaaag atacgtataa gaaaaaaagg aagtggagga actcataatg gcatgaaatc
    26521 tataactaag tgcctaggtt caaatgactt tccaagagtg agagtaggtg tatctaaacc
    26581 agaagctggt caagacttag cagactttgt actttctaga tttagaaaag aagaatctga
    26641 taacataaat gaagcattag aaaaagcggc agatgctatt gactcaataa taagagaaaa
    26701 tatagatatg tctatgaaca aatataatgg atagtgagag ggagatttaa atgaatgatg
    26761 tttttttata tcctttgcaa aattcaaaag agtataaaga tataataaac tgtataaaaa
    26821 atacaaaagg ctcattattg gttaatggac ttttaccagt acaaaaacct catatatcgt
    26881 attctatatt taatgattta tcacgacaaa tgatttttat aacaagtagt gatttagaag
    26941 caaagaaagt atacgaagat ttaagttttt atatggaaga taaagttgaa tatttaggtt
    27001 ttcaagatat ttatttttat cacttggatg caaaggacag aaacgaagaa gcgaagaaat
    27061 taaaagtatt gttaaaacta gcaaataaag aaaaaatcat tttagtaact tctattgagg
    27121 cagttttaag aaaatacata ccaaaacaag ttttgctcga tagtgtatct cattataagg
    27181 tgggagatag cttagactta gaaaagttaa ctgagaaatt agtttcattg ggatatgaaa
    27241 gagtttcaaa aatagagggg tttggtcaat ttagtataag aggtggaatt attgatgtat
    27301 tttcacttga atatacaaat ccaattcgta tggaattatt tgatgatgag atagattcca
    27361 tacgaacttt tgacgtatat tcacaaaaat caattgataa attgcaacaa ttctctataa
    27421 ctccatctag ggaatttatt tatcccgaaa aaactacaga tgcattagtt aaattaaaaa
    27481 aagaaacaac taaaaatact gatgaaggta catttcaaaa tatagattat atatcaagta
    27541 agacttattt tgaaggtgta gagaactata tagattatat ttatccagaa gaaaacaaaa
    27601 gtatatttac ttatttagca gatgatgcaa tagtatttat taatgatata acgagactta
    27661 aagaaagatg cgaaaattat ataaatgagt ttagagaaaa ttataaatta aatcttgaaa
    27721 gaggtctggc tattaaaaac caaggtaaat tgttatatca ctatacagat ttagagtact
    27781 tagtaaaaga caagtctgtg gtattaaact cgctattgcc aaagtctata aataactttt
    27841 ctataaaatc tataataaat tttgaaagta gagaagttcc tacatttaat ggaaaggtag
    27901 atttgcttgt agaagaatta aatcggctga aatataatgg atacaaaata attttagcaa
    27961 caaatacctt agaaagagca aataaattag gaaaagattt attggacaaa ggcttagaaa
    28021 cgaccatttc taaagataga gatattgaaa ttaaatcttc acaagttata attgtacctg
    28081 cacacataaa cagtggtttt cagtataaat ctataaagtt tgttgttata acagataacg
    28141 aaatgatagg tgtttataaa agagcgtcta agactagcaa taaaaaagtt aaaaagggaa
    28201 agaaaataga atccttttta gatttaagtg ttggagatta tgtagtccat gaaaatagtg
    28261 gtgtaggtag atacacagga attgaacaaa ttacagtcaa tgctattaaa aaagattata
    28321 tgaaaatagt atatcaaggt ggagataatc tatatgtgcc aatagaccag atggacaaag
    28381 tacaaaaata tattggtgca gaagtagaaa aagttaagtt aaataagctt ggaacaaatg
    28441 agtggacgaa agcaaaagca aaagttaaaa aagaaattga agatatgaca aaagacttga
    28501 ttgagcttta tgcaaaacgt gaaaaaatac aagggtataa gttttcaaaa gatacacctt
    28561 ggcaggcaga gtttgaatcg ttgtttccat atcaagaaac agaagaccaa ttaaaggcta
    28621 tagaagaaac taaaaaagat atggaatcaa gtaaggtaat ggataggcta gtatgtggag
    28681 atgttggtta tggaaaaaca gaggtagcta ttagaagtat ttttaaagct tgtatggacc
    28741 aaaaacaagt tgctgtattg gttccaacaa caatattggc ccaacaacat tataatacat
    28801 tcaaagagag gtttgaaaat tatcctttga gagtagaagt tttaagtagg tttaaaacac
    28861 caaaacaaca aaaacagatt attgaagatg ctaaaaaagg attagtagat attttaattg
    28921 gaacacatag aataatatcc aaagatataa atttaccaaa cttaggtctt gtagtcattg
    28981 atgaagaaca aagatttgga gtaaaacata aagaagcttt aaagaaaatt aaatctacag
    29041 tagatgtact tacactttca gcaactccta taccaagaac attacatatg tctttaagtg
    29101 gaataagaga catgagtgtt atagaagaac ctccacaaga aagacatcct gtaataacat
    29161 atgttacaga gagtaaggaa agcgtaattc aagatgagat agagagagaa ttaagtagag
    29221 gtggacaggt attttttgta tacaatagag ttgaacacat tgaagaaatg gcaagtatga
    29281 ttcaaaaact cgtaccagat gctagagtgg ctgttgcaca tggaagaatg acttctaaat
    29341 cattagaaaa tataatccta ggatttttaa ataaagatta tgatgtacta gtctgtacta
    29401 caataattga aactggtatg gacatatcaa atgcaaatac aatgattata tatgatgcag
    29461 ataaaatggg attggctcaa ctttaccaat tgagaggtag ggttggaaga agttcaagac
    29521 aaggatatgc atatcttttg tatgaaaaag ataaaacttt aagtgagata gcggagaaga
    29581 gattaaaagc tatcagagaa tttacagagt ttggttctgg ttttaaaata gcaatgagag
    29641 accttgaaat aagaggagca ggaaatattt taggttctca acaacatggt catatggcag
    29701 ttataggtta tgatttgtac gttaaaatgc ttaatgatgc aattaaaaaa gtcaaaggtg
    29761 agcctattgt ggaggaaatt gatgttgaaa tagatttaag tgtaaatgca tacattccag
    29821 ataattatat caaagatgag ttgattaaaa ttgaaatgta taagaaaata gcttctattg
    29881 aaaacaaaga agacatgttg gatatacaag aagaacttga ggatagattc tctgatatac
    29941 ctaaaccagt ccagacactt cttacaatag cttatataaa atcgttatgt aagatattaa
    30001 aaatagaaaa gataagacaa ttaaaagatg aaatattatt agttccaata acaaagtata
    30061 gaactaaaca gaaaataggt tataatatag ttacagaatt agaagaatta ttagaaaaga
    30121 tgtgcaaggt aaaataactt gtaaatttaa gaagggatgt tttaaatttg aaaaaagtaa
    30181 taacattggt aatagctatg attttggtag tatctgtgac tgcttgcagt tctagtaaag
    30241 gagaaactgt ggcaacagtt gaaggtacaa agatatcatc agatgaattt aaaaaaacta
    30301 tagctcttta taaagattct atggaacaaa cttatggaaa agatatatgg gataaggaag
    30361 ttgaaaaggg tgtaaaatac aaggacaagt ttaaagattt aattcttgac caacttataa
    30421 ctacagaagt aatatattct caagctaaaa aagataactt acttcctaaa aaagaggatg
    30481 ttgaaaaaag ttttaaagaa ttaaaagacg ctatgggtaa agatgaaaaa tataaagaac
    30541 aacttaagaa attaggtata gatgatgagt ttttaagaga ccaacaagag aaagatttag
    30601 caatgcaaaa ctatcaaagt aactttgcta aaaagactaa aatatcagat gaagaaatga
    30661 aaaaatacta tgatacacat aaagatgaat ttaaaaaaga tgaggtagaa gcttctcata
    30721 ttttattgaa aacagtagat gataataaca agccactttc agataaggaa aaagctgaag
    30781 ctaagaaaaa ggctgaagag gctcttaaag aagttaaatc aggtgaggat tttgcaaaag
    30841 tagcaaaaaa atattcacaa gatacttcag caagtgatgg tggtaaatta ggattctttt
    30901 caaggggtca aatggttgct gaatttgaag atgctgcttt ttctatgaaa aagggtgaag
    30961 tatctgattt agttgaaact caatatggat accacataat taaagtgaca gacagaataa
    31021 atgagcaaac atcatttgaa gatgctaaag aaactataaa agaccaactg ttgaaaaata
    31081 aatatcaaga acaaatagaa aaattaacta aagaagctaa ggtagaaaaa gatgaaaaag
    31141 taataaataa aatcacaatc taagacttaa aaaaagagta atatgcttaa aaaagctata
    31201 tctggataag atatagcttt ttttgtatat ttttcttaac aatggtaaaa ataaacttaa
    31261 agtaatttat ggagggatta aaaaatatga aagcaacagg tatagttaga agaatagatg
    31321 atcttggaag ggtagttatc ccaaaagaga taagaaagac attaagaatt agagaaggag
    31381 accctttaga gatattcaca gcaaaagatg gagaagtcat acttaaaaaa tactctccaa
    31441 taggtgaatt aaatgaattt tcgcaagaat atgctgaaac attagctgaa gtactaggat
    31501 atggagtgtt gataacagac ttagattcta taatagctgt gtctaagctt cctaaaaaag
    31561 attataaaga aaaaagtata agtgatgagt tagaagcaat aataagtgaa agaacagtaa
    31621 aatattcaaa agataaaaac attttaccat tattcaagga agaccaaaaa gaatatacta
    31681 gtcaagttat catgcctata attagtgcat ctggtgactg tataggttca attgtagttg
    31741 tttcaaagga taaagataca attgcagaaa gtgaagaaaa atctttaaaa attgctgcta
    31801 actttttagg aaaacaagtt caataatttt atcataaaga ccttttacat gaaggtcttt
    31861 tgaagttttt gtgtaaaata ttgaatatag cattaaaaat atggtatatt aaagtttgtt
    31921 atataaacag tatggaggtt atgttatgaa caataacaaa aataaagact cctttctaaa
    31981 aggagccctt atacttgggc tagcaggtgt aatcgtaaaa ataatgggtg gattttttag
    32041 gattcccctt ggaaacatga ttggttcaga aggaatggga tactatcagg ctgcttatcc
    32101 tgtgtatact ctatttttaa cattagctac tgctggattt ccaacagctg tggcaaaatt
    32161 ggtttcggaa aaagttgcaa ttggaaattt taaaggtgca aataagatat ttaaggtatc
    32221 acatacagtt ttattcatta ccggtataat atcattttgt atattatttt ttggagccga
    32281 ctacatagta acaaacgtaa tgaaaaatcc aggtgcgtta tactctatga aagccattgc
    32341 tccagcatta ctatttgttc ctgcaatgtc tgcatataga ggatattttc aagggagaca
    32401 agatatgact aagattgctg tgtctcaagt tgcagagcaa ttttttagag ttgtattagg
    32461 tcttactttg gcttacttgc ttatgaagag tctaggtcag acttttggtg cagcaggtgc
    32521 tatatcaggg gctacaatag gttcaatagc atctatgctt tatttggtgt ttgcttatat
    32581 gctagggaga aaagagcgta gagcagagat agatgcaagc caaagattta aagatgaaag
    32641 agtgtcttat atttttaaaa agcttttaac tgttgccatt cctataacaa taggagcatc
    32701 agtaatgcca cttgtaaata tgatagataa tgttattgta ataagaagac ttatggaagc
    32761 aggctttaca tacaaagtgg caaattccat gtttggtcaa ttaacaggta tggcaatggc
    32821 cacaataaat ttaccagcag tcataacaac tgcaatgagt atgagcttag ttccagcaat
    32881 ttctaaagca tatgcattag gaaataagtc aaaagctaga aaagatacta agagtgcagt
    32941 taaggtaact ttacttatag tattgccttg tgcgtttggt atggcttctt tagcaatacc
    33001 aataatgggt cttttatttc cacatgaacc atcttctgta ggaactattt tgtttactct
    33061 tactccatgt gtattatttt taggattaat acagacattg actggtataa ttcagggaat
    33121 gggaaaacca atagtacctg taattgcttt atgtgttggt atgctatgta aaattgtaat
    33181 aagttatact ttaacaggta taccagatat taatgtactg ggttcagcat ttggtacagt
    33241 tacagcatac tttgtggcag ccatgattaa cttactatat gttaaaaaac acatgaatgt
    33301 aaacttttct aaaaaagaat ttataataaa gccatttata acagttatga caatgtttat
    33361 aatggttaag ttgagttatg gtgctttagt tggattctta ggaaacagca tatctacaat
    33421 aattgcaata tgtgttggtg gaattgtata tgtagtagta atacttggaa taggtggaat
    33481 taaaaaagaa gagattctaa ctatgccaaa aggtgataaa ttatataaat tgttaaaaaa
    33541 agttaaactt ataagataat ataggaatat atatgcaaat aaaggaaata ctatacaaat
    33601 agtaaatctc cttatttgta taataatata aaaataaagt gaaatttgct aataggagtg
    33661 atgttatggg taagataagt attgtaggtc ttggaccagg agactattct cttataagcc
    33721 aaggtgtgtt agaatcttta ataaatagcc ctaaagtttt ttttagaaca gagaaacatc
    33781 caataattga taaacttaaa gaaactatac aatacacttc tttagactac ttttatgaaa
    33841 aagaggaaaa ttttgataat gtttatttga aaatatctga atttatcata gaagaatcag
    33901 caaagggtga cctagtgtat gctgttcctg gtcatccaag agttgcagag aagactgtaa
    33961 gtataataga gagattagcc aatgaaaagc acatagcagt agaaatattg ccttctatga
    34021 gctttgtaga cgctatgttt aactatttgg ctatagaccc atctgaggga tttaaactgg
    34081 tagatgcttt tgaaatagaa aattcatata tagacttgga tacaagtatg ataataactc
    34141 aggtatatga taaatttata gcatctaatg taaagttaaa acttatggaa cattatgact
    34201 ataatcaaga agtatgtata gtaaatggag ctggtgtcaa agatttagaa agtaaaaagt
    34261 ttataaagct ttgtgaatta gacagaaatg aaaacttttt tgactattta actagcttat
    34321 atatacccaa aagttctaaa aagatgtata atacagtaca tgacttagaa ataatagtta
    34381 atacacttag aagtccatct ggatgtgaat gggataaaaa acaaacacat caatcattaa
    34441 aaaattctgt tatagaggaa gcatatgaac tttgtaatgc aattgataat aatgatatag
    34501 atgaaatggt agaagagtta ggtgatgttc ttttacaggt tatattccat tgtcagatag
    34561 gaaatgagga gggctacttt gatttaaaag aagtagtaaa tggcatatgt aaaaaattaa
    34621 ttcatagaca tcctcatata tttagtggtg caaaattaga tatgtctaag tttgagaaaa
    34681 cttgggaaga attaaagaaa gaagagaaag gcgaaagtac tataacagat ggccttagaa
    34741 ggataccaaa acatttacca gctcttatga aggctagtaa agttcaacat aaagcagcac
    34801 ttgtcggctt tgattgggac aatgtagaag atgtatttaa aaaaattgaa gaagaatatc
    34861 aggaattact tgacgaacac agggtcggga atataaaata cataaaggaa gagctaggcg
    34921 atttattatt ttcgatagta aatctagcta gatttttaga catagactca ggagaagcct
    34981 tgaactgtac tacagaaaaa ttcataaata gatttgactt tatagaaaag agtgcaatga
    35041 acttgaataa aaaattagaa gacatgacac ttgaagagat ggatgagttt tggaatcagg
    35101 ctaaaaaaca ataaaattgt ggaaggaaat ttgtcaaaaa tctagaactt ttatcacaaa
    35161 gatagaagtc tatattataa acatttttag gaggtaaata tcgtgaataa agctgaatta
    35221 gtatcaaaga tggcagaaaa aagtggatta acaaagaagg aagcagaagc tgcgttaaac
    35281 gcatttatga gttctgttca agatgcacta gtaaataatg aaaaagttca attagttgga
    35341 tttggaacat ttgagacaag agaaagagct gctagacaag gaagaaatcc aagagatcca
    35401 gagcaagtta tagatatacc agcttctaaa gcaccagttt tcaaagctgg aaaaggatta
    35461 aaggatataa taaatggata attagaaatc ttgtgaaaaa gtgtggataa atttgccaca
    35521 ctttttctat agaaccaagg aaggtgataa ttatgagatt agataaattt ttaaaagtat
    35581 ctaggattat aaaaagaaga actgttgcta aagatgcatg tgataaaggg attgtcacta
    35641 taaatggaaa agttgctaaa tcttcttcag aggtgaatat tggagataca ttagaaatac
    35701 aatttggaga gaagaagatg aagtttaaga taaatgaagt aaaagagcat gtattaaaaa
    35761 atgatgcaaa agaaatgtac gaaattttag aataaaatta atagtcctct agtatattaa
    35821 tagtagtatt attgaaatat ttactggggg gatattatgg aacagaacat aactttgaaa
    35881 gatagatcaa agctggttat ttcgggagtc gagcacatat actcttttaa tgacaaaaga
    35941 gtagagctca aaacttctgt aggcgagatg gtaattgaag gcgaaaacct agatatgagc
    36001 aagctaagta tagacgaaaa tataattagc atagatggaa ctataaattc tatggtatat
    36061 gcaaagccta aaaagccaca agagagtttc ttgaaaaagg tatttagata gatgatacct
    36121 tttactcaag atgtaagcat tttttatgct actatttatg gtggaatact cataggggtg
    36181 ttgtttgatt tttatagagg gctaagaggg aattttaaat tcatcaatta ttttgctata
    36241 atatttgatg tattgttttg gtttctagct acggttataa tattcgtaac aataaacctt
    36301 acagaatttt ttgatttaag atattatcat tttgtggctt tgtttattgg ttttatttta
    36361 tactataaca ccataagtaa aattgtttta tcaattatca ataaaataat tcgttttgtg
    36421 agaaattctt ttaaaaaagt tacacattac atagtcagtt ttttaaataa tttgtattac
    36481 gttataatct attcactaca cctactattt gatattatat tttatatacc aaacatattt
    36541 atagcaacaa gaaaaagtat aaaaagaaaa tcaaataaaa aattaaagaa caagaaaaaa
    36601 tcaaaaccta agaagaaaaa aaataaaact aaaaaaaggg tgtaggaaaa atgaacttaa
    36661 gaaaaaaatt ttcaggacag attattgtta tcagtttatt tttaggaata tcaattttta
    36721 gtatgatgac aggatttgta tttgagtata caaaagctaa ggaatataaa aaagaaatag
    36781 caagtctaaa taaacaatta aaaaagactg aaatacaaat aaacagctta aagaaagatg
    36841 aaaaatcata tgaaggagac ttagaggata tagcaagaaa aagattgaat atggtaaaac
    36901 caaatgaaac agtttatgta gacataaatc gttgattaaa acttgctaat aagtttacag
    36961 atgtattgat tttaaaattt aagatttaat aaaaaagtca ttcagttaag gatggctttt
    37021 tgtttttatt gaggagggaa tttcataatg aaagtaggag ctatagatat tgggacaaac
    37081 tcaatgagat tattgatagc agattataga gatgggaatt ttataaacag aaaaaaattt
    37141 gttaatacaa ctagaattgg ttatggtgtg gatagtaaag gatatataaa agaagatgct
    37201 atcaaaagaa atataaaagc attaaaagaa ttttctgata agtgtaatga tgaaaagtgt
    37261 gaaaaaattt attgtatggg gacatctgct cttagagaca gtaaaaatgg agattacttt
    37321 gttaaattag ctaaagaaga aactggaata gatgttgata tagttacggg aatagaagaa
    37381 tcaaatttgg gctttaaggg tgttttagaa ggaatacaag gtgatgatac tattattgtt
    37441 atagatattg gaggaggttc aacagaattt attgtaggtg attcagaagg aattatattt
    37501 aataaaagtg agaatgtagg tgctcttagg atgacagaga ggtttttgaa gcacgatatt
    37561 attgaagatg aagagtttga agctatgagt tcatttatat atggtgagat tgataaaacg
    37621 ttaaatcata taaaagacaa aggattaaaa actcttatag gtataggagg tacgattaca
    37681 tcattatcag caatgaatca agaattagag atttactcta tggaaaaaat acataatagt
    37741 aaaatacaca aaaaagaaat agaagaaatt ctacaaaaat taataaagat gacattaagt
    37801 gataaaaaga acttaaaagg gctacaagaa aaacgtgcgg atattataac tgctggtgta
    37861 aaaatattga atataataat ggaaaaatta gaaatagaag aaataaccgt aagtgaatac
    37921 gataatttgg aaggcttaat atgtcaaaag tcaaaaaaaa tgtattaata ttttttgatt
    37981 tttaaaaccg attatgacaa aaaaaattac tcctctttgt tacaattctt aatataaaca
    38041 aaagaggagg tttttttatg caaagaagtg tagcagttgt gaagaataag actgtaaaac
    38101 caaatgtcat aaaattaata agcggatata ttgattttac tacggcggca ttcgctgtga
    38161 tgggattttt acttagtagg tctattatag tagactctgt agctccttta ggaattgcct
    38221 tttttatatg tgcatcaaag attgataagt acaagatacc tgtattcata tcaacattat
    38281 taggaatatt gttatcatct aatgattttg gaaatgtagc aaaatatgct gtgtgcctta
    38341 caatatttat ggctatgaat aaaaaattaa aattatcaga ttcaattgct aaggttgcat
    38401 ttataggtgc aataatacta cttccaatct ccatgggaca agctttcttt tcaaataggt
    38461 atgtatatgg tcttcttata gcaggagtag aagcaataat aatgtttata tctatatatg
    38521 tgttttctta tgggataggt ttaatggcca atgtcaacaa taggatgtct gtacggacag
    38581 aagaagccat atcaataagt ttactgctaa cctttagcat aatgggaata ggagatatat
    38641 ctctgtttgg aatatcaata aggaccattt tatcgaccgt attgatattg ctgacctcaa
    38701 tagtaggtgg agctactatg ggagcatcta gtggtgtaat tattggtgta gcatccattt
    38761 taaataatat gacatcagct gtttatatgg ggatatattc tttttcagga ttaataagtg
    38821 gagcatttaa taaaataaat aaatatttct gtatactagg atatattcta agttggacaa
    38881 taatatacct atatacttct ggtattactt ctaatatgat gcaattaaga gatatactat
    38941 taggttgttt gatagtacta gtattgccag agcgattatt taataagatt gaaaaattaa
    39001 taaaaagtaa tgtggcttca aatgaaatcg tatacgatta tattatgaga agcaaaaacc
    39061 taactaatag cagattgaac agtatttata aaacttatga tgaccttgca gatacatttg
    39121 ataagataag agaaaaagac aaagtgctag accaaagaga catagctaat gtaatagata
    39181 tgattcataa tgatgaatgt aaaagttgta gtatgagaag aatgtgctgg gagtcaagat
    39241 ttaatcatac atacactatg atatataaca ttcttgaaaa aattgaggag aaaggggagt
    39301 taagtctaaa cgatatacct aaaaatttta gaaaggaatg tatgaaacca gagtcaatag
    39361 tgaaaatatc aaatcactat tacaaaatgt ttgtattaga ttatgattgg agtgtgaagt
    39421 tttcagaaag tagaaagtta atagcaaatc agataagaag tatttcaaaa tccattaaat
    39481 ccttatctca agatttagaa ggcgacataa tgctagatat agaaaaggaa aaaaatatat
    39541 atgaacagtt agaaagatat gatataacag ttgataaagt aagttactta acaaaaagta
    39601 acagtgagtt tgaaatatca atagaaaaga aaacttgcca tgatggatgt atgtgtgaag
    39661 ataaaattgt aaatataatt tcagacctag taggggaaaa tatgtctgtt agaaaaatag
    39721 gatgtcactg tttaggaggt aaatgcaagg caacattcgt taaatctcaa aaatataagg
    39781 ctgttacaga agtttcagca atgtcaaggg atgggcatat cctttgtgga gacaattaca
    39841 catacatgga gataaatgat ggtaaataca tgatggctat aagtgatggt atgggaaaag
    39901 gtaaaaaagc atatgaagaa tcttctgcca ctattgatat actggaaaaa atgatagatg
    39961 caaaaattaa agatgagata gtcattgata ctataaataa tatgttgttg ttaaagtctt
    40021 cagaagagat gttttcaact ctggatttgg gaattttaga tttaaaacga ggttgtttag
    40081 aaactataaa gatgggagcg tgttctactt atataaaaag agaggatgga gaagttgact
    40141 taatatcgtc atcatcatta cctgtgggta tattatcaga tgtaaaaatt gatagaaaaa
    40201 atgtaaaagt taaggaagga gactatgtta taatggtttc tgatggaatt gtagatgcag
    40261 gaagaaataa caatttagga gacaattggt taatatactt cttaaagaat atagagacta
    40321 caaatccaaa ggaaatttca aatttaatat tggatagagc attagagtta caagcgttac
    40381 aaattgaaga tgacatgact gttttagtta cgaaaatatg tacaaaataa ggtttacaag
    40441 attatgtgta taagatgtgg ataacttgtg tataatatgt taatagactt ttgaaaaaag
    40501 tatagttatt aacaaaaata acatataaat caacaaaatt tgttgataag tatggcagaa
    40561 atgttaataa aatgtaaaaa aagaaaaaac aaaagctatt atcaaaagag attcatgttg
    40621 aaataactaa atttctaagt aaagaaaacc tgtgaatatg ttgattatta tgtggataac
    40681 tattaattcg atatttattt atatagtatg aataattgaa taatatatct aaaaaaagcc
    40741 atcctattat taagtaaact tattgaaagg atggcttttt atgaatagaa aattgtgcaa
    40801 agcaataata cctttattta tttctatttt ttgcatgtta ataataagta taaaagctga
    40861 ccaaggatat caaaagaata atgatagagc aattactgtc atgaataata atgatactga
    40921 agatgatgct gaaaaaaaac aagaaataga ctatgaaaaa acatgttgga atttgcaatc
    40981 tttgtttaaa agtgatgacc aatggaaaaa agaacttaag agttttgata aagatactaa
    41041 agagttgaaa aattacatag gtaaagtaac aaaatcgcca acacatttgt cttttgcctt
    41101 aaacattaaa gaaaaacttg atataagact aaataaactt tctgcctatg taaaattaaa
    41161 acaagatact aataaaaatt cgtataagta cttagatatg aatgatagta tgagtaaatc
    41221 ctatgggaat tatatgggga tttgttcaga tttagagtta gaaatactaa aattatcaaa
    41281 caaagattac aagaaaatta tttcaaataa aaatataaat aggaaatatg gtgcatattt
    41341 aagagatata agaaggaata aagtacatta tttggatgat aaatcagaag atattctaag
    41401 caaagtatct tcaataagta gtcttccaag ccaagtatat gaattattta gaaatatgga
    41461 tagaaaaact aatttgacac cagctcaata tgcgagtgaa ttagagtcat cagatagaga
    41521 aaatagaaaa aaagcttatc aagatgaact tataacttac aatgataata taaatacaat
    41581 tgcaggttta attactggtc aagttaaaaa gaatattttt tattctcagg aaagaggtta
    41641 tgagtcttct ttagaaatgt acctggaatc tgataatgta gatgaaaaag tatacaataa
    41701 tttgataagg actgtaaata gtaatatgga cagtctacac aaatatatag atttaagaaa
    41761 aaaagtatta aaattagata aggttcattc atatgatatg tttgtgccta tggttaaggc
    41821 tgttgataat aatattaatt atgaaaaggc acaaagtata gtatattcag cgttgtcacc
    41881 tttaggaaaa gaatacaatg atgtagtata taaagctttc aatgaaaaat ggatagatgt
    41941 ttattcacat gaaaataaag taagtggtgg atactgctta tctgtttatg aaaatcatcc
    42001 atatatactt ttaaattata ataattcatt aggttcagtt tcgactttat cacatgagct
    42061 tggacatgct gtatatgaat atttaagttc taagaatcag aattatttta attcaaatcc
    42121 gtctatattt actcacgaag ttgcatctac aacgaatgaa gcattacttt acgagatgct
    42181 tataaaagaa gctaaaggca atgatgaaaa agcttattat ataacacagt atttagactt
    42241 gataaaagat actctttata cacaaactat gtatgctgaa tttgaaaaaa ctatacacga
    42301 gttagtagaa aaaggaaaaa gtgtaaacgc attagtatta aatgatgttt ggggacaact
    42361 tcttaaaaaa tattatggac aaagctatga attagaccaa ttatctaagg tcggatgggc
    42421 tagaatccca catttttata atagtttcta tgtctataaa tatgctacag gttgtagtgc
    42481 aggagtaagt tttgcccaag acatcctaaa gaatggttca gataactata tgagctttct
    42541 taaaaaggga agttcagatt atccaataaa cttgttaaaa tcaagtggca taaatttaac
    42601 taatacaaaa cccatagaaa atacaatcaa aaaatttgat agtttagtag cagaacttga
    42661 aaagctatta taaacattat tgtaattcac atggtaaata tatcgaatta atgataaaat
    42721 atactaaaag tttaaagtcc tatcaagata ggacttttta tttattaatt agtgggagat
    42781 gattctatgc aagttaaagt taaaaaggct gtaataccag cagcaggttt aggaactaga
    42841 tttttgccag ctacaaaggc acaaccaaaa gagatgttac caatagttga taagccaact
    42901 ctacaataca taatagaaga agctgtggcc tcaggaatag aggaaatact aataataaca
    42961 ggtagaaata aaaaatctat tgaagaccac tttgataaat ctgtagaatt ggaacttgat
    43021 ttagagaaaa aaggtaagaa agaattgttg gaggtagtac aaaacatatc taacatgatt
    43081 aacatacatt atataagaca aaaagagcca aaagggttgg gagatgcaat ttattgtgcg
    43141 aggcatttta taggtgatga gccgtttgca gtaatgttag gggatgatat tgtagataat
    43201 gatgtacctt gtctaaaaca actaacagat gcatatgagg aatatagaac tacaatattg
    43261 ggtgttcaaa aggtaaatca agaagatact aataaatatg ggataataga agcaaaaaat
    43321 atagaaggta gagtttacaa ggtaaaagac atggtagaaa agccagagtc aggaaaagct
    43381 ccttcaaata ttgctatttt aggtagatat ataataactc ctgaaatctt tgatattctt
    43441 aaggatttac caccaggtaa aggtggagag gtacaactta cagatgcttt aaaaatatta
    43501 tctaagaaag aagctatgta tgcatacaat tttgaaggca agagatatga tgttggggac
    43561 aagctaggat ttttagaagc tactgttgac tttgctctaa agaaagaaga ccttaaggaa
    43621 gattttataa aatatttgaa acatgtatgt agtgaatttg ataaaaataa caatgtgtta
    43681 tacaataata cagatgaaaa aatagattta attgaagtca atgaagctag agaacttgaa
    43741 attcaaaaat aattaatgaa aggtgatgta tggtggcaaa atcaagtcag aaaagaatta
    43801 atagaataat agctgttggg attgtgggaa tcctcgtaat aacgagtata acagctgttt
    43861 tgagtatgat tgcaactatg taataagata aatgacagca gttgatatac ataaatataa
    43921 aaaccatata taacttgatg tagtcaaaaa cttacaatat tttagttata tatggttttt
    43981 agaatttata aatgtaaaaa aaaattttta taatagttgc atattttaaa ggaaaatatt
    44041 attataagat ataaatatag tgataaattt ataaaaacgt gctaagattg gacttgaaat
    44101 acagtaatat agtaggtaat aatgttttaa attttattta ttgaccataa tatataaata
    44161 atgatattat tagacttaac tgtttaaata aaacttttct ttttcatgcc ttcaatttcc
    44221 aaaataagta tgtatataaa tgaatatata gatgaggagg atttttttga ttttagatga
    44281 taaagtatta gaaggattag gtagtaagac cttagttgag ctacgagaga tagctaaaga
    44341 attaaaaata aaatcaatta ctacatataa gaaaaatgag ttaattgaga ttataaattc
    44401 caaaagtaaa aatgaggaaa aagtaaacga agagactaaa gaaaaaaatg aagatattaa
    44461 agaagacata aagaatagaa aagaaaatat agagcgtgaa agtatagaaa aagaagttga
    44521 atataagagc cctaccaaat catacaacaa gaaagaaata gatacccaaa atatttccca
    44581 aaaccaagaa catagaaatc aaagaaatga ctataataag acaacgagtg taaatgatag
    44641 taataagtca tcaaatatat ctaatagaat ggtcagaaat aataataaaa actattatat
    44701 gcctaaacaa gttgatgaat ctaagatagt agatgaattt aacacatcaa aagaggatga
    44761 agtagtaggt gtacttgaaa ttctgccaga tggatttggt tttttaagag gttcaaatta
    44821 tttgtctact gaaggagatg tttacgtttc accatctcaa ataagaagat ttaatatgaa
    44881 aacaggagac aagattaagg gaataactag acatccaaaa agtggagaga aatttagagc
    44941 acttttatat gttcaaaaaa taaatgatga aaaccctgac acagctatac aaagaaatgc
    45001 gtttgagaca ttaacaccaa tatttccaga agaacgactt acactggaaa ctaatagaaa
    45061 tgaaatagct acaagaatta tagatttaat ttcacctata ggaaaaggac aaagaggatt
    45121 gatagtagca cctccaaaag ctgggaaaac agttctttta aaaagcgttg caaatagcat
    45181 agctaaaaat catccaaatg ttgaacttat agtactcctt atagatgaaa gaccagagga
    45241 agttactgat atgaaagaat ctattgaagg agacgttata tattccactt ttgaccaagt
    45301 atcaagtcat catgtcaagg ttgcagagat ggtgttaaat agagctcaaa gattagttga
    45361 acatggtaaa gatgtagtaa ttttactaga tagtataacc agattggcaa gggcatataa
    45421 ccttacaata tctcctacag gtagaacttt atctggaggt attgaccctg gtgcacttca
    45481 tggacctaaa aagttctttg gtgcagcaag gaatattaga cagggtggtt ctttgacaat
    45541 attaggaaca gcattggttg aaactggttc taggatggat gatgtaatat ttgaggaatt
    45601 taaaggtaca ggaaatatgg agctacattt agatagaaaa ttagcagaaa aaagaatatt
    45661 ccctgcaata gatatttaca agtctggtac aagaagagat gatttattgc ttgatgatga
    45721 agaaaagact gctctttgga gattaagaag agaaatgagt aataattcag taatggaaat
    45781 tacagataaa gtcattgaac ttataaagag aactaaggac aataaagagt ttgtaaaatc
    45841 aataaagaat ttgtaaaatc aataatacaa atgcaaatat ggattatata aactaataga
    45901 aaaatcactt gaatggcata ataagttgtg ctataataat gtagttgata atgtaaaaag
    45961 aaagttaata taattaattt ttttaaataa agagaagagg tgactataat gcaaaaggaa
    46021 atacaaccaa aatacaatcc agttgaagtg cgttgtgctt gtgggaacac atttgttgct
    46081 ggttcaacta aggacgaaat aaaagttgaa atatgttcag agtgccatcc attctataca
    46141 ggaaaacaaa agaacataga aaaaggtgga agaatcgaca aattcaagaa aagattcaaa
    46201 atggactaat ttttataaaa gtggagttgg aggaagccaa ctcctatttt attacaaaaa
    46261 aacattcata ttctacttta aattgtcttt ttgtattata ataataaata tgtaaaattg
    46321 agtattctaa atacaataaa atgaggtgag tggatggcaa aacaagctgt aggtgggcaa
    46381 gctgttattg aaggtgtaat gatgcagtca aaagacaaaa gagcggtagc tgttagaaaa
    46441 agtgacggag aaatagcact aaaagaagat agaataaaaa gttgggtaag agataagaat
    46501 atagataaga taccttttgt tagaggttct tttgttatga tagatacaat gattcaaggc
    46561 ataaaaagtt taaacttttc ttcagagttt tttatggaag aagctgaaga ggataaattt
    46621 gatttattta taaagaaaat ttttaaagat aaggcaaatg atataattat aatattttca
    46681 ttggtcatag caatgttact ttctgctgga ttattcattt ttatacctac attggtagga
    46741 ggagcttttt ctaaagtaat gcctaatgat tttatgctta atttaataga aggaatcata
    46801 aggatagcta tattatttgc atatatagta ttgatttcac gaagtaagga tatagaaaga
    46861 gtctttcaat atcatggagc agaacataaa tctatatatt gctatgaaaa tgatttagaa
    46921 cttacagtag agaatgctag aaaatttaaa agattacatc ctagatgtgg tacaaatttc
    46981 ctatttatag ttatggctgt atcaataata ttgtttgcat tttttggttg gccaaatcca
    47041 attcttagaa tatttatgag aattatatgt gtgcctatag tagcaggttt atcttatgag
    47101 gtaattagag ttcttgggaa atatgataat ggatttacta aaattattgc atatccagga
    47161 atgatgttgc aatattttac aacaaaagag cctgatgatg agcaattaga ggttgctttg
    47221 gaagcattga aagcagtagt agattagaga aaaggatatt tgttatgacg ataaaagata
    47281 taattattaa atattcagat aaactaaaag atataagtga tactccaaga ttagatacag
    47341 agttattgtt acaaaaaaca ttgggtgttg atagattata tattcactta aatctgaata
    47401 aagagctaac tgaagaacaa aaaacaaaat ttatgggttt tgcagaagaa agattaaatg
    47461 gaagaccaat agcatatata gttgaaaata gggagtttat gggattagat ttttttgtta
    47521 aggaaggcgt attgattcca agaccagata cagaaacttt agtagaagaa ataattgaaa
    47581 tatgtagaga aaaaaaagat gtaagtatat tagatatagg aactggttct ggagctatta
    47641 caataagttt agcaaagtac attgaaaatt ctaaaatcat gtcttttgat atatcagaga
    47701 cagcattaga aatagctaaa aaaaatgcta ttataaatga agttggtgaa aaaataaagt
    47761 atattaattc tgatttattt acagcaataa gtgatagtaa cataaaattc gatatcatag
    47821 tttcaaatcc accatatatt aaaaaacaag atatagaaac tctgcacaaa caagttaaag
    47881 attatgaacc atataatgct cttgaaggtg gagaagatgg attagacttt tatagaagaa
    47941 taacagaaca aggtaagaaa tatttaaata agtgtggaat attagcatat gaagttggtc
    48001 ataatcaagc tgaagatgta ataaatatta tgaaaagtaa tggatataaa aaaatataca
    48061 caaagaagga tattcaaggc attgatagag ttgttatagg ttacaatata tgatataatc
    48121 aacaattgat atgttacatt taatcgaggt gaaagtatgt taaaaaaatt agaggtattg
    48181 gaagatacat ataaagactt aagtgaaaaa ataggtgatc cggatgtaat aaatgatcaa
    48241 aaagtttggc agaaatatat aaaagaacat gctgacttgg aacctattgt tatgaaatac
    48301 agagagtata aaagcgtttt agatagtata aaagaatcaa aagaaatttt acaagaagag
    48361 tcagatgaag aattaagaga attagctaaa atggagttag ctgagatgga agaaaaagta
    48421 gcacctctgg aggaagaaat aaaaatacta ttattgccta aagaccctaa tgatgataag
    48481 aacgttatag ttgaaattag aggtggagct ggaggagatg aagcagctct atttgctggt
    48541 gatttattta gaatgtactc aaggtatgct gaaagaagaa gatggaaaat agaattattg
    48601 agtgcaagtg atactggagt tggtggatat aaagaagtat cttttatgat aaaaggtaaa
    48661 ggtgcttatt caagattaaa gtatgaatct ggagttcata gagttcaaag aataccatca
    48721 actgaatctg gtggaagaat acacacatca acttctactg ttgcagtatt accagaagtt
    48781 gaagatgtag aggttgaaat aaatccaaat gatttaagaa tagatgtttt ccgttcttca
    48841 ggtaatggag gacaaagtgt caatactact gactctgctg ttagggtaac acatatacct
    48901 acaggtgaag tagtatcttg tcaagatgga aaatcacaat taaaaaataa agagcaagct
    48961 ttaaaaatat taaaagctag actttacgat aaagcattgg cagaacaaca caaagatata
    49021 gcagcagaaa gaaaaagcca agtcggaaca ggagacaggt cagaaagaat aagaacatat
    49081 aatttccctc aaggtagaat aagtgaccat agaataaatt taactttata taagttagat
    49141 gcattcttag atggagatat agatgaaatg atagatgctt taattactgt tgaccaaaca
    49201 gaaaaaatga ctgcaatata agtaatgttt taaagaagct gtttcttcct agaaattatt
    49261 ttctattttg aaacagctat tttgcatttt aggtcatgtt atcatattct attgtatata
    49321 attgttatat acaagtatag gagggttata tgatactaaa agtaacaata attgggcttt
    49381 tggcaggtgt tatagggaca ggattaggag gagtaatatc agccattttt aaaagagagg
    49441 tcgataaata tctaagtttt tttatgggat tatctggtgg aataatgttg gctgtagtgg
    49501 tgtttgattt aatgaaagaa tccatggaca aaatggggat aatcaataca gttatattta
    49561 cgtttgttgg agctctcata actatgtaca taaaaacaaa attagatgtg tctggaaata
    49621 tggcatctgg atatcttata ttcataagta tattactaca taatttacca gaaggactag
    49681 ccattggttc ttcttttatg tcaactgaaa gtttaggtat tacacttgct atagttatag
    49741 gtctacataa tataccagaa gggttagcta tggcattagg cttagtttgc aataaaatga
    49801 aactaagtaa agttattttg tttactgtaa tagctggatt acctatgggt ctaggaagtt
    49861 ttttaggtgt ttattttgga ggagtattta cttctcttat aggagtattt cttgctacag
    49921 caggaggtac tatgatgtat gttgtattgg aagaaatttt tccacattca aaaagtgtgt
    49981 attgtataat tggtttcttg ttaggtacaa tgattgtaaa ttatatataa aattaaaatg
    50041 aaagcaggca tacaaaagat gaaaactatt ataagtaata ttgatataaa taatattgat
    50101 tatgaggaaa taaaaataca agcaaaactt cttagagaag gtaaaacggt tatttttcca
    50161 actgagacag tatatgggtt gggtgcaaat gcattagatg aaaatgcagt aaagaagatt
    50221 tatgaagcta aaggaagacc tagtgataat ccattgatag tccatatata tgaaaaagaa
    50281 gaagtatatg acttagccaa agatataagt gataaagcta aacttgttat agaaaagctt
    50341 tggccaggac ctataacaat tatattaaat aaaaaagata taattccata caaaactagt
    50401 ggagggcttg atacagttgc aataaggatg ccttctaatg ttattgccag ggcaataata
    50461 aaagaagctg gtatacctat tgcagctcct tctgcaaata tctcaggtcg cccatctcca
    50521 acaaaagcaa aacatgtata tgaagagatg aatggaagag tggatggaat agttttagga
    50581 ggagacagta attttggatt ggagtcaact gtattggatt taacagaaga gactcctatg
    50641 atactgagac caggaagtat aactaaagaa gttttagagg aacttcttgg agaagtaaag
    50701 cttgacccat cattaagtaa aaaagaagat aatcaaaaag ctaaagctcc tggaatgaaa
    50761 tataaacatt attctccaaa tgcagatgta tatataatat ctggaaaaag agaaaatgta
    50821 gcaacaaaga ttaatgatat gataaaatct aatagagaca aaggtctaaa gacaggtgta
    50881 atgtgtatta gtagaaaccg agagttttat gatggagaag taatcgattt aggaaactct
    50941 cttgaagaag ttgcaagtaa tctgtttgat gctcttatag aaatggacaa aaagggtgtt
    51001 gatgtcatat attcagaaga gttccccaag actggagttg gacaagcaat tatgaataga
    51061 ctactgaagt cagcaggata caaagtgata aaggcttaag agggagttgt aaaatatgaa
    51121 tatacttatt gtgtgtacag gtaatacttg tagaagccct atggctgagg ctatactaag
    51181 gaaagcaatt aaagaatctg gaagaagtat tgaagaatat tctatatcat cagcaggtat
    51241 atctacagca aatggaatgg gtgcttctga aaattctata gaagtactaa aagaaatcgg
    51301 tatagactta tcaaatcata gaagtaaagt aattactaaa aaattaattg atgaatcaga
    51361 tataatacta acaatgacta aatctcataa agaaatttta gtacaagcag ttccaaaatg
    51421 caaagaaaaa gtgtatactt ttaaagggtt tgcaaataaa aatgaggaag atatatctga
    51481 cccatttggt ggaaatttgg atatttatag aagcactatg agagaaataa tgtattctgt
    51541 caacgaaata gtaaaaaaaa tatgaaacgg aggataaaca aatgaagata ggattaggct
    51601 gtgaccatgg aggatataat ttaaaaaaag aaataataag ctatttagaa agtaaaggta
    51661 tagagtgtgt agattatgga actaataatg ctacagattc tgttgattat cctgtttatg
    51721 gagaaatagt tgcaaattca gttataaata aagaagtaga ttatggaata ttatgttgtg
    51781 gtacagggat aggaatatct ttagctgcta ataaggttcc tgggataaga tgtgcagttg
    51841 tatcagatgt attttctgca aaaatgtcaa aagcacataa tgatgcaaat atgttatcat
    51901 taggcgaaag agttttagga aaaggtctag cacttgagat agttgaagca tggattaata
    51961 cggattttga aggtgataga catgctagaa gagtaaacat gattaaatca atagaagaaa
    52021 aacataataa ataataaaaa ttacagtttt atcaggagga attatcaaaa tgagcaaagt
    52081 ggtagagaca aaccatccat taatacaaca taaattaact ttaatgagag ataaaaatac
    52141 tggttctaaa gactttagag agcttttaac tgaaattgca atgttaatgg ggtatgaaat
    52201 aacaaaagat ataccattaa aagatgtaga aatagaaact cctattcaaa aaacatcatc
    52261 taaagtagtt gcaggtaaaa aattagcaat aattccaatt ttaagagcag gtcttggaat
    52321 ggtagatgga ttagtgagct taatgccagc agcaaaagtt ggacatgtag gactatatag
    52381 agacccagaa acattaaaac cagttgaata ttattgtaag cttcctcaag acataggaga
    52441 aagagatata atagtagttg accctatgct tgcaacaggg ggttctgctg tagcagctat
    52501 agatttactt aagtctaaag gagctaaaag tataaaatta gctaatttag tagctgcacc
    52561 agaaggtata gcagaagtgc aaaaatatca tgatgatgtt gatatatatg ttgcatcagt
    52621 tgacgaaaga ttaaatgaac atggatatat aattcctggt cttggtgatg ctggagatag
    52681 attgtttgga actaaataaa aataaataac tataaatatt tattagggac tttcttatat
    52741 agtgtatata attttatgta agaaggttct tttttatata ttttttctca aaataggaat
    52801 tattgaattg ataaatttct cgaattgaga ataaaaagtt tttaccaatt aaaaaagatt
    52861 gcttatttta caaggtttta tagaatagta gtctatttta atgtggcatg ttagttgctt
    52921 acgattatag gtattaagtg tattagttaa attgagaatt gaatattaat aatattattg
    52981 ggagtggtaa tacatgaaga attgtatagt agtaagaggg gctggagatt tagcaacagc
    53041 agttattcat aaattacaca acagtggttt taaagtagtt gcgttagaaa tagaagagcc
    53101 attagctata agaaggcaaa tttctttttg tgaggctgtt tatgaaaaaa aagtaactgt
    53161 tgaaggtgta gaatgtatat tttgcaatac tgaaaaagaa atagaggaag ttttatctca
    53221 acataaagta gccttgatta tagacccaaa aggagaaaaa ttatctaaat taaatccaat
    53281 cgttttggtt gatggaatac tcgctaagaa aaacttaggt acaactaaag atatggcacc
    53341 acttacaata gctttaggtc ctggattttg tgcaggagaa gatgtagatg ttgttataga
    53401 gacaatgaga ggtcataatc taggaagaat aataactaat ggtagagcag aaaaaaatac
    53461 aggagtacca ggagcaataa aaggtttttc taaagagaga gttatgcatt cacagtatga
    53521 tggcataatg gaaaatgtat gtaagatagg tgatatagtt gaaaagggag acctactttg
    53581 ctatattaat aattatgaaa aaaaacatga agttagagca acaatatctg gagtccttag
    53641 aggtttatta aaggatggag caaaggtaaa caaaaattta aaaatactag acatagaccc
    53701 tagaaaagaa gaagttaaga atgcttttac aatatcagat aaagctagat gtatagctgg
    53761 aggggtttta gaagccatac taaacaatgg gatttatccc caaaattaat ttatattaga
    53821 taaagctatt aatattttat taagatgata taataaattt aatagattaa agagagcata
    53881 tgacgggtat ttaaaattta aatggtaaaa atgctctttt tttattggac attcaattaa
    53941 catgtgtata taatataaaa ataagtattt atttaggttg aaatctaata aatatataat
    54001 accagtacat aagcaaagag gagaaaagta aattatgaga ccatcatggg atgaatactt
    54061 catggaaatt gcagaagtag tgaaaaagcg ttcaacttgt attagaagac aagtaggagc
    54121 tgtaattgta agagacaaac aaatattaac aactggatat aatggttctc cgagaaatct
    54181 tgaacactgt gaaaatattg gatgtaagag gcaggaatta aatattccct caggagaaag
    54241 gcatgaatta tgtagagcat tacatgctga acaaaatgcg ataatacaag ctgctcataa
    54301 tggtattagt gtagatgggg caactttgta tgttactaca agaccttgtg ttttgtgtgc
    54361 aaaaatgtgc ataaatgcag gtatattgaa aattgtttat gaaggtgatt atccagatga
    54421 tatgtctaca gaattattaa aagaagcagg aatagaactc ataaaatttt agtggataaa
    54481 atttatatga caataaatgt caaattatga cactgaaata caatggtatc aaatgattaa
    54541 aagaaagtat ttgaaaaatc atcagattag tagtaagata tattttataa ttaaataaat
    54601 ttatgagata gattaattac atgttattta ttttataata atgagggata ctatttatga
    54661 gtaaaagaaa tacacataca gaaattgcaa agttgtttag tttaataagt caaataggat
    54721 tgatgattgt aatatcaata ttaggatgta ctttcttagg aaaattttta gatagtaaat
    54781 tgaatactac tccagttctg acaataatat ttttattgtt gggtgttgga ggtgctttta
    54841 gtggtgttta taaaactcta atagtatata cacgaaggaa gtgatatatt ggatataaaa
    54901 ttacttaacc aggtaaggtc agtaacaaaa ggtatagtaa tatttgatgt agttttaatt
    54961 gtgctaatgt tgataacatc aacattaagt aaaccagtat tattaggact tatatttggc
    55021 tctataatag ctatgttaaa ttttagatta ttggcgatat cactggaaaa atcagtgaca
    55081 tttcctgctg ggaaggcaca agcttatgca tcagcacaat atgctgttag attatttata
    55141 atggcagttg ttctaattgc atcagctaca gtaccacatt taaatataat cggaacagca
    55201 ttgggattat tgagcacaaa gtttgtcata ttatcaaaaa actttataaa taaattaaag
    55261 aggaaggagg cataagtatg aatcaagcgc attattatct cataggaaac tttaagatta
    55321 gtgaaacaat actagtaagt tggggaataa tggccttttt agcgatagca tcttattttt
    55381 tgactagaaa cttaaaaaaa gttccaacta gtaaagttca aatattctta gaatttgcag
    55441 taggtggtct tgctaaaatg gttacagatg ttatgggtaa aaatacagta aaaagaatgc
    55501 catatatgat accatacata ggaagcttgt ttttattttt cgcatgttca aatttaagcc
    55561 cattacttgg attaaggtca ccaacaaccg acttagacac aactgctgca tgggcaatga
    55621 ttacattttt tatgatttat tttgcaggag ttaaatttaa ggggttatca tactttaaag
    55681 aattggctca accacttgcc ataatgttac caatcaatat tataggtgag ttagctagac
    55741 caatatcatt aagtttccgt ccatttggga atattttagg tggaacaata ataatgggtc
    55801 tgttttatca acttttaggt tttatatcta gtttaatacc aggattatca ataccaattg
    55861 gagaaatatt aataccagtc ccattacact tgtactttga cttatttgca ggattgttac
    55921 aagcatatat atttataatg ttaacaatgg tatttatatc aagtgcagga gagttagatt
    55981 aagaaatcaa cttatattaa gataaaaata aaataattta aaaaattaaa gttttaagga
    56041 ggaaataatt atggaaacag caatagtagc ggcagcatca gcaataggag cagggatagc
    56101 agttgcaaca ggtataggtg caggtatagg tcaaggtata gcagcagcta aagctgcaga
    56161 agcagtagga aaccaaccag aagctaaagg tgatataaca tcaactttat tactaggagt
    56221 tgctatagca gagtcttcag caatatatgg tttggttata tcaataatac ttctattcgt
    56281 aaatccattc tttaaatact taggaatgta gatttagtcc ttaaatattt tgagataggt
    56341 ttataggtaa atcatctcaa acttataatc atgagatgaa ctttatcatc tcgtgattat
    56401 aagtagttat gatttatgga aaggaggata attatggaaa aagcactagt aggtataacg
    56461 tgggaatttg tgttccaaat tgttaatact tttataatat ttttacttct gagaaagctt
    56521 ttattcaagc cagtattaaa tataattgaa tcaagagaaa atgatattaa aagtgatttg
    56581 gcagaaggcg aaaaagcaaa aaatgaaggt ttagctctga aaaaagaata tgagtcaaaa
    56641 atcaattttg ccaaggatga aggtcaagaa atcataaaac aagcaactat aagagcagag
    56701 cagaaatcag atgatatagt aaatactgca aagaaagatg ctcttgatat aaaagaaaaa
    56761 gcaaacaaag atatagaaca agaaagacaa aaggttataa atgaaatcaa aaatgatata
    56821 tctaacatag cactacttgc agcatctaaa gtcattgaaa aagatttaga taaatctaaa
    56881 catgaagaat tgatagaaaa ctttataaaa gaggtagggg aagctaaatg ataaatgtaa
    56941 tagcaaatag atatgcagaa gccctatttc aattaggaga agaagaaaat tcaactgatg
    57001 tcttattcaa agaactagaa aaagtagtag atatgatgac taaagttagt aaagatttct
    57061 acaaagtttt aaaatcacca ttagtttcaa aatcagaaaa gaaaaacttg gtagaaatta
    57121 tttttagtaa agaagtaagt tctaacataa aaaacttttt aaaagtttta gtagataaag
    57181 atagaatatc atatttagaa gatatcgaat tagcttataa agaattatta aataagaaaa
    57241 acaatgtaat tgatggagta gcaataagtg ctattccaat gagtgaaaca gatattaaag
    57301 aacttgaagt caaactttca aataaataca acaaaaatgt tactatagaa aatgttgttg
    57361 acaagactat actaggggga gttctagtta gaattggaaa tgaacaaata gatggcactg
    57421 tgaaaactcg tcttgataag atgaaagaaa agctatctga agtaatatct taagaatggc
    57481 ggtgaaccca tgaacttaaa acctgaagaa ataagttcta taattaaaca gcaaataaaa
    57541 aattatgaga ataaagttga gttgacagat acaggtagtg ttttaacagt tggggatggt
    57601 atagcgagtg tatatggatt agaaaaagct atgtctggtg agttgctaga gttcccaggt
    57661 gaaatatatg gtatggcact taacttggaa gaagaagtag ttggagctgt tatccttgga
    57721 gatgacagtg aaataaaaga aggcgatata gttaagagaa ctggaagaat agttgaagtt
    57781 ccagttggag aagctttaat aggtagagtt gtaaactcac taggtcagcc aatagatgga
    57841 aaaggaccta tagcgtacac aaaaactaga ccagttgaat ctgaggcacc tggtataata
    57901 gatagaaggt ctgtttatga accattacaa acaggaatca aatcaataga ttcaatgata
    57961 ccaattggta gaggtcaaag agagttaata ataggtgaca gacaaactgg taaaacatct
    58021 atagttattg acactatact aaatcaaaaa ggaaaagatg ttatctgtat atatgtagca
    58081 attggacaaa aacgttctac tatagctcaa ttagttagct ctttagaaaa aggtggagct
    58141 ttggattata caatagtagt ttcagcaaca gcatctgaat cagcaccact tcaatatata
    58201 gcaccatatg ctggagcagc tatgggtgaa gaatttatgt acaatgggaa acatgtactt
    58261 atagtgtatg atgatttaag taaacaagct gttgcatata gagaaatgtc attgttactt
    58321 agaagaccac caggacgtga ggcatatcct ggagatgtat tctatttaca ttcaagatta
    58381 cttgaaagag cagctaagtt atctgatgaa ttgggtggag gttcaatgac tgccttgcct
    58441 ataatagaaa ctcaagctgg tgacgtttct gcatatatac caacaaatgt tatatctata
    58501 acagatggtc aaatatactt acaaccagag ttattctatt caggtgtaag accagcagtt
    58561 gaccctggta tatcagtatc aagggttggt ggttctgcgc aaattaaagc aatgaaaaaa
    58621 gttgcaggaa cattaaaact tgcatattca caatatagag aacttgcagc attctcacag
    58681 tttggttctg acttagatga agatactaaa aagagacttg ctcaaggtga aagaatcgtt
    58741 gagatattaa aacaaggtga gcatcaacca ataaaagttg aaaatcaagt tatgataata
    58801 tatgctgtta taaataatca tttagaggat attccgatag ataatatagc tagatttgaa
    58861 tcagaattgt atgcatttgt agataataat tatccggaaa tatcaagaaa aatattaggt
    58921 ggagaagact tcactcatga tttaacagat gcaataaatg aatttaaaga aaagtttgtt
    58981 gttgaagtat aatccttttt aaaaaaggag gtaaattaat tggcaggagc tggaatcaaa
    59041 gcaatcaaaa cacgtatggg aactgttgaa agtacaaagc aaataactaa agcaatggaa
    59101 ttagttgcat cttctaagct tagaaaagca aaagaaaagg ctgaagattc aagagaatat
    59161 tttaatactt tatacaatac agtaaaagat attgcccaaa caacacgtgg aattagaaat
    59221 gaatttttaa aagaaagaga agtaaaaaat aaatgctatg tagtagtagc tggagacaga
    59281 ggacttgcag gtggatacaa ttctaatgta ttaaaggaat ctgtctctca tatgaaaggt
    59341 aaaaaggaaa cagtgattac tgttggaaag aaagcacatg aatttttttc taaaagaaac
    59401 tacaatatag caaaagatat aaatagtgtt gaagcttgtg gatatgaaga tgcgacagaa
    59461 atttcacaaa ctattattga cctgtataaa aaaggtgaag tagatgaagt ttatattgct
    59521 tatacaaaat ttaaatctgc tttatcacaa gaagttgaaa ttttgaaatt gcttccatta
    59581 gtctttgaaa aagatacaga cgaacaagga atggcagtaa aagaagaaaa acaaaataga
    59641 tcaaaagtac aatatatacc atcagcagaa gcagttttaa gttatattgt acctcagttt
    59701 atatcaggta cagtatatgg tggaattttg gaatcttttg catcagagca aggtgctaga
    59761 agaactgcaa tggaatcagc cacagacaat gcgaatgaaa tgctatctaa tttagagtta
    59821 agttataaca gagcaagaca gtcagctgta actcaagaaa taacagagat agttggtgga
    59881 gttgaagctc taaaataggg aggttaggaa atggcaaatg taggtaaagt agtacagatt
    59941 gtaggagctg tactagatgt taagtttgat agtgaacaaa gcctacctaa cttattaaat
    60001 gcgttagtaa taaagttagg agataaagaa atagttgcag aagttgctca acatataggt
    60061 gatgatactg taagatgtat agctatgagt gcaacagacg gacttgtaag aggtatggaa
    60121 gttgtagata caggagggcc aataagtgtt cctgttggag atgaaacttt aggtagaata
    60181 ttcaatgttc ttggaaaacc agttgatggg aaaccagctc caaaatctgc acctaaatta
    60241 cctatacata gaccagcacc agcatatgat gaactagaaa ctacagcaga gatactagaa
    60301 actggtatta aggtagttga cttactagct ccatatctaa aaggtggaaa aataggtcta
    60361 tttggaggag caggagtagg aaagacagtt ctaatacaag agcttataaa caatattgct
    60421 aagcaacatg gtggtatttc tgtgttttca ggagtaggag aaagaacaag agaaggtaac
    60481 gacctttatg gcgaaatgag tgagtctgga gttataaata aaacagctct agtatttggt
    60541 caaatgaatg aaccacctgg agcgagaatg agagttgctt taactggact tacaatggca
    60601 gaacatttta gagatgagca aggacaagac gttttacttt tcgttgataa tatattccgt
    60661 ttcacacaag ctggttcaga agtttcagca cttctaggac gtatgccatc agctgttggt
    60721 tatcagccaa cattagctac tgaaatgggt gcacttcaag agagaataac atcaactaag
    60781 aaaggttcaa taacatctgt tcaagcagta tatgtaccag cagatgactt aactgaccca
    60841 gcaccagcta caacattctc tcacttagat gcaaaaacag ttttatcaag acaaatatca
    60901 tcactgggta tatatccagc agttgaccct cttgaatcta catcaaggat tcttgaccct
    60961 agtatagtag gaaaagaaca ttatgaagta gctagaggtg tacaatcaat acttcaaaga
    61021 tataaagaac ttcaagatat aattgctata cttggtatgg atgaattatc tgatgaagat
    61081 aagttaatag ttgcacgtgc tagaaagata caaagattct tatctcaatc attcacagtt
    61141 gctgagcaat ttacaggaaa tccaggtcaa tatgttcctg ttaaagagac tgtaagagga
    61201 tttaaagaaa ttcttgaagg gaaacatgat gacttaccag agtcagcatt cttatttgta
    61261 ggaacaatag aagacgctgt tagaaaagcg aagggaagta tgtagtcatg gccagtgaat
    61321 tttcattgaa agttgtaact ccaagtaata ctttttatga tggagatgtt gaaatgatag
    61381 tagttagaac tacaaaagga gacaaagggg tgttgaaagg tcatatacca tttgtttcag
    61441 gtatacctgt aggaacttta aagataaaaa aagagggaag ttttaaagag gcgaaaatag
    61501 cagaaggatt cattaatgta actcaagata aaactgtcat attgacagaa tctgctgagt
    61561 ggatttaata aattaattta gagtataaaa agtcattttg accaaaaaaa cttttgggta
    61621 aaatggcttt tttgcgtttt ggtgttaata aaataacgat aaaattgtaa gtttttagtg
    61681 ataataaatt cacaaaatgt ttaaatttac tgccaaatta tgttgtaatt aataagataa
    61741 agaagattta gatagctttt atgtaagtga aaaagtcttc atataagaaa tacatataaa
    61801 atttttataa aatttaaaaa atttgcttag ttgtgtagta taataaataa tataatagat
    61861 ttgtatataa tattcattac aaataatttt aaaagttaca gttttattat aatgattatt
    61921 aa
//
LOCUS       DAMJVK010000009        61723 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.009, whole genome shotgun sequence.
ACCESSION   DAMJVK010000009 DAMJVK010000000
VERSION     DAMJVK010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 61723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61723
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.009"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(442..2226)
                     /locus_tag="PC414_000601"
                     /pseudo
     CDS             complement(442..2226)
                     /locus_tag="PC414_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860844.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
     gene            complement(3010..3198)
                     /locus_tag="PC414_000602"
     CDS             complement(3010..3198)
                     /locus_tag="PC414_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463014.1"
                     /translation="MNNKKKAFLISGAINVISLIIVFSLFHYNIIENSTQNMIITVIL
                     IFIFEMIKIKIIEKYYKL"
     gene            complement(3224..4363)
                     /locus_tag="PC414_000603"
     CDS             complement(3224..4363)
                     /locus_tag="PC414_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDF2463015.1"
                     /translation="MVIKLTIEEYKNKYFTGNKIVLSINSFDNKELYYHETENILNLI
                     NEIDKLTSSKIFKTIEDKNDLALESYYSSVIEGAFSTRKIAKSIIRGKMKPSNKSEYM
                     IYNNHRALEYGLDNLDELYSHKFIYDLHHILGENCLDSEEYEYRTEKVYVCDSKGEII
                     HTGLEPLKIYDFISKLIDFMENSKVSNLIKSAIIHFYFVYVHPFSDGNGRTSRALSYL
                     YLIDKGYDTFKDFSISYMISKNRTKYYKAILDVENKGNNLTVFIEFMLKSIIQSINEM
                     RNMHDRKSLESILKEELFENDITLSATEEHILKYICNKDNYSMTLENYIKKNKSRYLK
                     AGIKEIELVDQLMEVFNNLEEIEILSKEKNIYKVNDKYLKMLDID"
     gene            complement(4900..5739)
                     /locus_tag="PC414_000604"
     CDS             complement(4900..5739)
                     /locus_tag="PC414_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDF2463016.1"
                     /translation="MSDIKNEVSKKTLSKKDVIKSWLRWFFFAQSNYNYERLQSTAFS
                     HSMLPVLKKLYPDKEELKQEVETHLAFFNTEPICGCVIHGITIAMEEEKANGADEISG
                     DGMNAIKTGLMGPLAGIGDTLTQGVITPIVLAVCIGLTEGGASIAGPILFVIAQYIIM
                     TSISFGMWTNGYKYGKKAVESILQGGIVNKVIEGASILGTLVMGGLVGRFINLSTPIS
                     YTSGDFKFSLQTDLLDKIFPGLIPLVLTLLVLFALKKGLSPIKIMFILIVVGAITGIL
                     GLF"
     gene            complement(5763..6521)
                     /locus_tag="PC414_000605"
     CDS             complement(5763..6521)
                     /locus_tag="PC414_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2463017.1"
                     /translation="MHISLIQAVLIAIFYYLSWSPWLTYVGFFTWNRPLLAGFVTGII
                     LGDPVQGAIIGAGINMIYLGFISAGGAQMGDPAFAGYVGTALAIASKLDVSTAMAIAV
                     PLGTVATVLWIGKMTVNSFFAHWADREVQKGNVDRVAFINIVPPQILLFAMSFIPALL
                     VVYFGPNAIDGMLEVMNDNVLHVFNVIGAMLPALGIAMNLKLIGNKFTMPFFILGILM
                     AVYFKVDIIVISVIGVILALTITSIKYGKDSATS"
     gene            complement(6568..7041)
                     /locus_tag="PC414_000606"
     CDS             complement(6568..7041)
                     /locus_tag="PC414_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2463018.1"
                     /translation="MSNIALTRIDDRLIHGQVITAWCKITSAKRIVIVDDLVVKDPFI
                     VQVLQMAAPSTVKVEVHDVESGAEVLKSHNGDENLIVLVKYPKTVLGLVNSGVDLKEL
                     NVGGMGAGLGRKSFYKNISVSDEEKEIFRELISKDVKCFIQIVPDAKKIDVGTLL"
     gene            complement(7070..7486)
                     /locus_tag="PC414_000607"
     CDS             complement(7070..7486)
                     /locus_tag="PC414_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2463019.1"
                     /translation="MIHMAGIIIATHGNMAKAMLESAELIVGKQENVETLGLNHGDSI
                     DEFSRKLEDGIEKYKDEGVLVLLDFYGGTPFNTSAIVINKFAKVCELECLTGVNLPML
                     LELFLNRDTMKLKDLKNLCEEVGISGIKDVKTVLKL"
     gene            complement(7533..8585)
                     /locus_tag="PC414_000608"
     CDS             complement(7533..8585)
                     /locus_tag="PC414_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDF2463020.1"
                     /translation="MKSVRFYGIRDTRVEDVDVPKILEKDDVIIKVKVAGICGSDISK
                     YSKTGPHMVGEILGHEFSGEVAQVGKEVRSFKIGDRVAVCPAMPCFECDECKKGLYSR
                     CNNVAIIGNKELGGCFAEYTKVKERNLIKIPDEISYETAAALEPVCIAGHGLFRSEAK
                     VGDTVVVLGTGPIGLFSIQWAKIFGSTKIIAVDVFDEKLDLAKELGADICINAKEKNI
                     VEEIKRLTDGDGADIVIESAGTPLTCGQVLLLAKKGGTVLYAGVPYGDVALTREQFEK
                     IVRSELTVKGTWFGNSFPFPGKEWSAGLYHMQKGDMNVEKLVTHRINLEEVPAYFEKV
                     YKRDIFFGKIMINIDN"
     gene            8981..9661
                     /locus_tag="PC414_000609"
     CDS             8981..9661
                     /locus_tag="PC414_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDF2463021.1"
                     /translation="MKKVYQGKTKDVFELDNGNYLLKFKDDVTGKDGVFDPGENSVGL
                     SIDGIGRANLETSVKFFEILNNAGIKTHYVSANLEEATMEVLPAKVFGNGLEVICRYR
                     AVGSFLRRYGSCVEEGAKLDCYVEATLKDDDRCDPLITSEGLEALNIMTQAQFDSMKS
                     MTQKISNIVRDTIAEKGLELYDIKFEFGFYNDEVILIDEIASGNMRVYKDGVIVDPMD
                     LTALLLDK"
     gene            complement(9794..10114)
                     /gene="sugE"
                     /locus_tag="PC414_000610"
     CDS             complement(9794..10114)
                     /gene="sugE"
                     /locus_tag="PC414_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HDF2463022.1"
                     /translation="MKWLFLFVAGLFEVFWAFQLKNSHGFSKLTPSILTVVGMIVSFY
                     FLSLALKHLPLGTSYAIWTGIGTVGTAIVGMAILNEPISISRIACIGFIVVGIVGLKL
                     LSTN"
     gene            10496..11323
                     /locus_tag="PC414_000611"
     CDS             10496..11323
                     /locus_tag="PC414_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HDF2463023.1"
                     /translation="MNFYKIAVCQMITTENKIENINHAVDMVTEAAINGAKIVVLPEM
                     FNCPYENKYFPKFAEEYPGETTTILSKLAEKHGIYLVSGSIPELEDGKIYNTCYVFDK
                     NGALIGKHRKMHLFDIEVTGKVSFKESDTLTAGNDVTVIDTEYGKVGIAICYDIRFPE
                     LSRLMALKGAEIVILPAAFNMTTGPAHWELSIRMRALDNQIFYVGAAPARNMNASYIA
                     FGNSRISDPWGRIIAQADEKECIIYADIDRDLIPDIRQQLPLLKHRRTDLYELNTLK"
     gene            12145..12819
                     /locus_tag="PC414_000612"
     CDS             12145..12819
                     /locus_tag="PC414_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2463024.1"
                     /translation="MNNKILVVDDEEGIITLLKDYFEMNGYEVYTAQSGKDALKKISK
                     NPDIILLDINMPEIDGIEVCNRIRDYVSCPILFLTARIENTDKINGFRAGADDYIVKP
                     FDLDELGARVSAHLRREQRKSNQTKIKFYNDLTIDYEKRLICIYNNQISLSKKEFDIV
                     ELLSMNPGQVFDKEKIYDRVWGYNSNGNSDVIMEHIRKIRIKLSKHTLENYIETVWGV
                     GYKWIK"
     gene            12807..13967
                     /locus_tag="PC414_000613"
     CDS             12807..13967
                     /locus_tag="PC414_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2463025.1"
                     /translation="MDKINNMSLKKAFFVLTLSSLIIASVLTTFAYILLNNLYNSIQD
                     KYLKTPINTGIITIVQNNVNQFSDYDKTLLNIINILQLVLPLIFFIGLLLLADIIFYK
                     VKLKTPIEILNKGAMEISNNNLDFCLEYNNNDELGNLCNAFEKMRSELNKNNIKMWTM
                     INDRKQLNAAFSHDLRNPLTVLKGYSNYLTKYIPTGKLSDEKILSTTQLMSEHINRIE
                     YYVENMSNAQRLEDLVVSKSMSNINKFIENLDENISIIAKQEGKSFTLKSQINNINLF
                     FDENIILRVVENIISNAFRYARNNVSILIYLEQELLTFVIEDDGIGFSKESLKLALRP
                     FYRDKTLNDSNAHFGMGLYISKILCEKHGGSISIENNSTNSAKITAKFSTRN"
     gene            14183..14710
                     /locus_tag="PC414_000614"
     CDS             14183..14710
                     /locus_tag="PC414_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014118918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HDF2463026.1"
                     /translation="MKSFANNITFSRIVFALLMLSSTFSSLPFWICYFWCGISDILDG
                     LIARKLRQQSGIGAKLDSIADFIFAVTILVTVGKNIALPMYLWWCIVAIALLRLISYS
                     IGFFKYRTFSSLHTLMNKITGISIFAFPLLYICFGLDVAGIMICIVAFLSSFEEIAIM
                     IKSKELNRDIKSIFM"
     gene            14996..15664
                     /locus_tag="PC414_000615"
     CDS             14996..15664
                     /locus_tag="PC414_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2463027.1"
                     /translation="MIIKAKQLSKIYGSNNNKVIALNNVNLEINSGEFVSVIGPSGSG
                     KSTLLHILSGLDNPTSGQVLLDDKDIYKHTEKELSALRRKSFGFVFQQFNLLPVLTAS
                     ENISMPVLLDKKQPDKDYLNEISSLLGIADRLNHLPHELSGGQQQRVAIARALIAKPD
                     IIFADEPTGNLDSKSGSEVMNLLIKTSKQFGKTLVVITHDDRIAKLADRKISIIDGVL
                     MEVK"
     gene            15667..18135
                     /locus_tag="PC414_000616"
     CDS             15667..18135
                     /locus_tag="PC414_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2463028.1"
                     /translation="MKNYLSLSIKELKSQKFMTTFIIIAIVLSTIMTTVVGQSIGILQ
                     NLRIEQARSFNGDRHVSFHQLTKNQVDDIKKDDRVYQAGTSITIGSSKIKDSGISVLV
                     KEYDKTGLSNYPKLMKLKSGHLPKDKNEIALDENTLKLMGIKPRLGVTIPMNLDISLL
                     NDTIPPYNYTANFKLSGILEDDYTGYVSGIVNGIVGKGTSENLLPKRYILSSLDFKIK
                     QQEKFQEIVNQLAKKINLSHNSIQYNWIYLNALKIQFEKDENSSNSDGISMIILVSLF
                     VALLVLLASGLVIYNILKISVTKKIKEYGCLRAIGAEPNQIYKIVILQILILCTVAIP
                     IGAVIGIISSKGITGMVTNILNPDILLANDNKEITELIHKNTTAYIFPLVLSTNVSLI
                     FSFISALPSAIYASHVSPKIAMVGSTTKIKRKIKREKTIKNFERHLAWLNLKRNKGRT
                     IITILSLFMSITVFVALSEFSNVLDVSRSVSNLKEGDFSLTNEISGFDKSTLDKIAKM
                     KNVNRTSFIKYSEYKQGEIDTDINFENSGEMLKIIGIDEQTLKDLMPSITDSILEDFK
                     NGSICFIKNPIAISTPGVKTRYTNLKPKDSITINKKQLDIYSTVDKMFFLQGNGWING
                     VDVIVYDSVYNTLTNKNKFNQINIYAKDKSKLEQIRLSIEQICENNPGSHWISYIESD
                     KQLKESFKQIEFLAWAVILFVGLIGTLNIINTTHTNINTRTNEIGVKRAIGMSNRSLY
                     KMFLWEGVYYGIFAAIFGSIAGYASAIIINMATIEKLDFTNIPITSILQATIISVLAC
                     IIATLIPLRKVKKMNIIDCIDINL"
     gene            complement(18375..19799)
                     /locus_tag="PC414_000617"
     CDS             complement(18375..19799)
                     /locus_tag="PC414_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2463029.1"
                     /translation="MKNQSLITQFRHTFIFIIIASIVATVITYVFALYLYIHSLNKDI
                     YPPNYYERQVPRIEKYINEKNIALLSQSNEEGLKRTIRGDDMLYQVVDNNGNILYGTN
                     PKKLFKTKEELFNNFINKTVRKGGYIHTVPIKGDNGKIEGAVILFYQVKITFANIRGR
                     FVFAVIIMALFSPFLYIVGFTRWLSKRFVKNINQPLHLLIDASKKIKEKDLDFEIDYY
                     SDNELGKLCSAFSEMKDELKGSLSAQWKMEQERVEMVEALAHDLKSPLSIILGYTDAL
                     IGNNTDDNEKLHRYLTVIRENTEKSAALVQKMQYTSDLEKSNIQLNLVPINLPEFLRQ
                     KVQDYELQAHQKEVELILKMQGNIQSPIQIDVDRLTRIFDNIISNSLQYTPSGGNISI
                     TVKDEKNCISYEICDSGRGFSSKDLKKALDKFYRGDEARQTKGGHSGLGLYIVKQLVE
                     QLGGSVKIENSKSGGACVKFWHSI"
     gene            complement(19818..20510)
                     /locus_tag="PC414_000618"
     CDS             complement(19818..20510)
                     /locus_tag="PC414_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2463030.1"
                     /translation="MSKILIIDDEMDLVMLLEDELKAKGHEVLIAYDGQAGIELSKQE
                     PDLIILDIMMPKMNGFEVCQAIRDDVLCPIIFLSAKQSETDKIKGLTFGGDDYVTKPF
                     GLRELMARIEANLRREKRSQYINEENKRSTLYFGKLCLYMKERAIKIDGKNIDLTKIE
                     YDIVELLALHAGQVFSREQIYEKVWGCDSEGDSSTVVERVKKIRAKFSAVTPEKEYIS
                     TVWGIGYKWNKM"
     gene            complement(20562..21341)
                     /locus_tag="PC414_000619"
     CDS             complement(20562..21341)
                     /locus_tag="PC414_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HDF2463031.1"
                     /translation="MIKLLSSEWLRAKRTAVQWLTLCMPIIFSLCVVFYLTTKSGSTQ
                     EFAFEGFFTVWTVFIIPIGVGILAGFIVQEEELAGNFNGFLCVGISRVRLYLGKFLFL
                     LFCLTICTFIATLILCIGMSIAVPSGATIWLFLSAAGLVVIGTLPLLAIHLWVSFAWG
                     MGASIGISIGGILMAAILGLTSVGEKVWAFVPWTWPVKLGMLPGIYFIKEAGTISTEA
                     FYSKVMQTASIELIVVAIGLIIFLIGGVIWFKMWEGRKSYE"
     gene            complement(21338..22108)
                     /locus_tag="PC414_000620"
     CDS             complement(21338..22108)
                     /locus_tag="PC414_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HDF2463032.1"
                     /translation="MNALQSELLKYKRTFMGKLIVFFPVSFAAYAFIMQSTLMQNPLS
                     QTTSWAWQNLLALIFNWWSFLFLPIGFALFATLVAFQEKKAGNYRALRTHNVSPMTLW
                     INKVIAMAVYSFISTLVLIVVTIITGLILKAGPVPFGQIIGASIVCWVVSLAILPLQL
                     WLATWKGMFLSMGVGALGMIFGVLAATKPFWIAVPWSWAVRMVCPIINIHPNGTILEA
                     GDPLLNTSVIPIGIVVSLVVFIVLTALTAAWFNRRDDK"
     gene            complement(22105..22809)
                     /locus_tag="PC414_000621"
     CDS             complement(22105..22809)
                     /locus_tag="PC414_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HDF2463033.1"
                     /translation="MKDLILETKNLSKRYGEQMAVNNVSLQIERNSIYGLLGPNGAGK
                     STTLKMLVGLLRPTNGRIIFDRKPWKRESLAKIGSLIESPALYGNLTAEENLLVHTKI
                     LGIPKDKIYEVLEIVNLKNTGKKRASQFSMGMKQRLGIAIALLSDPELLILDEPTNGL
                     DPFGIQELRELIASFPKKGITVILSSHILSEVAQVVDNIGIINGGRLLFQGMPDPNEN
                     LEEFFTDVILKGGQNR"
     gene            complement(23064..23576)
                     /locus_tag="PC414_000622"
     CDS             complement(23064..23576)
                     /locus_tag="PC414_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463034.1"
                     /translation="MKKISQEKISTHIQEIPQEKIEQWKSLYEKYKSILKVNSKSVLE
                     IIEFLKRKYVIVEETSEEFKHVVINNIKLNKPFSQKISSDKELKPIVFTIPNEGNAKK
                     LYDTQEEIYSDCKIIVGMEFETGCVFVEGSDELNDEITSFKGLDKYDINNYYLVANYI
                     RCLKKYKLID"
     gene            23902..24159
                     /locus_tag="PC414_000623"
     CDS             23902..24159
                     /locus_tag="PC414_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463035.1"
                     /translation="MILNRAELFRGYSLNRFNEICNILSVNKIKYKYKVKNNAYSKKP
                     FFNEITLGNLGQKEDFSYEYLIYVHKDDYEQAESLINSKLR"
     gene            24302..24928
                     /locus_tag="PC414_000624"
     CDS             24302..24928
                     /locus_tag="PC414_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HDF2463036.1"
                     /translation="MKINIKSKLSEFIKQNNLFDDSRIISYLPNITIETDKIKTIMIN
                     EVVPTNTNDDFYSVDKDADYLKTTIPLFDSAGIKVSNINDILDMGIYITNAVKLPKSE
                     YTITRDTIKLHMPILEEEIKLFKNLEVVMLMGDVAKKSFNMITKKHIKKNVIPSISTY
                     KLRNNELYYDNLRVFPSYIMTGGNILIEKSKFEMASEDIKKMFEIING"
     gene            complement(25175..25954)
                     /locus_tag="PC414_000625"
     CDS             complement(25175..25954)
                     /locus_tag="PC414_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901940.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HDF2463037.1"
                     /translation="MNSKIYYFSGTGNSLVVSNILKETLNGSKEVIPLAIHRKENNID
                     INEDILVIVFPVYFADVPDIVKSFVEKLNFNINTHIYAIATCNGVAGHSLFTIDKLLK
                     KRGKKLSAGFLINMPGNALITPPDVEEERLKNQTNRVAEIAKCINNLEIGNIEGKNNL
                     KSHIDSVVLRLVGKNMQIATTKFFAENKCNGCGTCKRVCPVENISIVDKKPKWGNECE
                     RCLACFHWCPKEAINVKKSFLTNRKKYHHPDITVKDIFLDK"
     gene            complement(26022..26483)
                     /locus_tag="PC414_000626"
     CDS             complement(26022..26483)
                     /locus_tag="PC414_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2463038.1"
                     /translation="MGGARDYLNGGKMKDFEKLYEATTKLVSTSLKVKDDLKKMFKQN
                     GYDITTDHYALLRFLWEQDGISQIDLCEKSCKDKSNTTRILDVMKNKGLIVRKVDVKD
                     RRKFQIFLTDLGRELEEPLNEIASIYATETFKSISDEELPLFTDILDRIGK"
     gene            complement(26459..27136)
                     /locus_tag="PC414_000627"
     CDS             complement(26459..27136)
                     /locus_tag="PC414_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2463039.1"
                     /translation="MIITVINGSPRKNGATSKVLTYLYKDIERLIPDVKINYFDLSEV
                     NPSYCIGCLNCYKMGKCINQNDKVEYIHDIITKSDGVIFGSPTYGSSVTGLFKVFTDR
                     AHMMLERLLYRKPCIAVTTYENARGSKAISFIKSMVLDSGGYVCGSLSIKTGFNQNPI
                     TEKVESKIQKVSKKFIYCIEEKKNPPVLSQIYNFIAINAVLKPMAFKDIEQYKGIIDR
                     WEEQGII"
     gene            27369..27755
                     /locus_tag="PC414_000628"
     CDS             27369..27755
                     /locus_tag="PC414_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2463040.1"
                     /translation="MKKLPHISEAEYQVMKIIWKYAPISTTEVIEKLVETSTWSPKTI
                     QTMLLRLVKKGALTYEKNSRVFVYTPLVKEEEYVATESSSFLNRFYNGTLNSMVLNFL
                     ENDKLSEDDIEELREILNKRTTKGDK"
     gene            27758..29557
                     /locus_tag="PC414_000629"
     CDS             27758..29557
                     /locus_tag="PC414_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaR1 family beta-lactam sensor/signal
                     transducer"
                     /protein_id="HDF2463041.1"
                     /translation="MYAPFFIRFLLSTLTLSILIAVILLAKKVFKKHISAKYQYNIGF
                     ILLIMLIIPFVPLKYINLGNVFNYLYTFNQLGSSTNRTSMIKNSPNSIINNSNALNDF
                     SVSLNQVNFDSLNILLLISWIIGILIMIAITIHCNIKIKNMKKSIQILRDEEIVFIFE
                     KCKKDIGINKDFILCKSPIINTPITFGYFKPHIILPDKSISKLSLKDIKYILLHELQH
                     FKNKDILINYIMCFLQILYWFNPLVWYAFKEMRIDREIACDISVLKKLDKDSHIEYGQ
                     TLINFVDNISRPSNLTLTSDIGGSKKQIKKRLLKIMDFHNESNTLKFKSILIFTLIAL
                     IVFGNIPKLSIMADINEKYRPTNEQTVYEDLSQYFKGFDGSFVMYDLKSKQYHIYNKE
                     KSKTRISPNSTYKIYSALLGLENGIITRNNSHIPWDGKHHSNSSWNEDQDLFSAMENS
                     VNWYFQTLDKKTGLKNIQTYLRQIGYGNYDVSGGISNFWLESSLKISPIEQVELLESF
                     YTNKFKFEDKNIETVKDSLLISKKDGVSLFGKTGTANINGKDVNGWFIGYVEKNENTY
                     FFATNIQSKDYSNGSSASKITLSILNDKKIYNP"
     gene            complement(29912..31786)
                     /locus_tag="PC414_000630"
     CDS             complement(29912..31786)
                     /locus_tag="PC414_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2463042.1"
                     /translation="MNKYNKIANELIKIIGEDNIISITHCATRLRVMVKDREIINDKK
                     VEKVDEVKGVFFTSGQYQIILGTGIVNKVYAEVEKMGLKTLSKKEQDELVKNNETGFK
                     KVMRTLADIFVPIIPVIAATGLFLGLKGCLFNDNVLGLFGMSSANIPLYIQTLVSVLT
                     ETAFAFLPAIIVWSAFKVFGGTPVIGLVIGLMLVSPILPNAYSVADPSNEIEAIMAFG
                     FIPIVGCQGSVLTAIVTAFIGANLEKWFRKHMPNVLDLIFTPFFVMLITMLVILLGVG
                     PIMHTIELKMVDIISLLIDLPLGIGGFIIGFTYPLAVITGLHHTYVMIETSLLANTGF
                     NALITLCAMYGFANIGTCLAFMKKSKNNQVKQTAVGAMLSQLFGISEPVLFGIQLRYN
                     LKPLIIMCASSGLGAAILSILHIQSNSYGLAVLPSYLMYIYDGYNLITYLLASIFVVA
                     FCFIVTCLFGVPKEAINEDEDEELVFNENNENFVSPAKGKIVALENVPDETFSKKMLG
                     DGFAIDIIDGKIVSPISGKLETVVSSGHAFGIKGTNGEVLIHVGIDTVALNGDGFDIA
                     VKQGDMVKQGDVLVNVDLKRIHELGKSTLTMVLFPDGKKVNILDINKDVKIGQRICVE
                     "
     gene            complement(31791..33455)
                     /locus_tag="PC414_000631"
     CDS             complement(31791..33455)
                     /locus_tag="PC414_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDF2463043.1"
                     /translation="MEKKWWQKEIVYQIYPRSFQDSNNDGIGDLQGIISRLDYLKDLG
                     ITSLWLCPIFKSPMDDNGYDISDYLDIASEFGSMEDLKELIKKSKDRGIKIILDLVLN
                     HTSDEHPWFQEALKNPESPYHDYYIFKKGNDLPNNWRSVFGGSVWEKVENRDEYYFHS
                     FGKKQPDLNWENPILRSELYQMINTWSQMGVAGFRVDAITFIKKNLSFQSLESDGVDG
                     LVKCTKTVRNQPGIEKMLYELKENTFKKYDCMTVAEAAGISYDQLGDFIGEDGFFSMV
                     FDFKHADLDVASGSEWFKRISWTIPDLRKCILDSQMYVQEHGWAANFIENHDQPRATT
                     KYLLQYERNDDAVKMLGAMYFFLRGTPFIYQGQELGMTNFKRDTINDFNDLSSIDQYY
                     RSIDEGYSEEEALEFVNLRSRDNARTPFPWADKENAGFSDTTPWLKVIENYKDINAQS
                     QINNSESILEFYKKMIKLRQDSEYSDCLIYGSIEPIDIESDDIIAYRRQLDLVVIDCY
                     YNFSDKPVEIRVEEKYDIVFQNLDDINLLNKTVRLKPFHAVLLKVR"
     gene            complement(33469..34290)
                     /locus_tag="PC414_000632"
     CDS             complement(33469..34290)
                     /locus_tag="PC414_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF2463044.1"
                     /translation="MKLYKLLILDIDGTLRDEVYGIPESAKHAIRLCQKNHCSVVICT
                     GRSMGTIQDDVLSLGVDGYIAGGGNYIQYHGELLYNQSFNQRLIKEVVCLLKNREVAF
                     SIESQEKVFMNQKAKEIFETMNQLKGTNSCINKQHIQEKITYENNIEEYKSQDIHKIC
                     LWSNEKVFDEVKDILQDKMELAQRDISSQYYEIIQKDFHKGKAIKRLQERLGVTQKET
                     ICFGDGQNDIVMFQASDVTIAMKNSHQQLKDIATSICEDIFDNGIYKELKRRNII"
     gene            complement(34389..35153)
                     /locus_tag="PC414_000633"
     CDS             complement(34389..35153)
                     /locus_tag="PC414_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2463045.1"
                     /translation="MKIEGLFNKYYNSFTANDKYICECILNHKEDCIKLSIDEFADKY
                     HISTSALSRFAQKLQLPGYSELRVIIRLNETEVEYKNQSKHQLMDCYNKVINDIEKKD
                     CSIMFERMKKANRIIVFGEGYSQERVAKEMKRIFLPTGKIIYDVYGYDMIQALNHFIK
                     TDDMIFFISLNGDASTIIDFAKEMRMKGIYMVSITKMISNPLARLCDENLYIQSISIS
                     VDKQLDYDVTTPYFILIELLYIKYKMYLESEHIPRK"
     gene            complement(35150..35899)
                     /locus_tag="PC414_000634"
     CDS             complement(35150..35899)
                     /locus_tag="PC414_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2463046.1"
                     /translation="MRLESLVNQNYDNLNENDKIIIKQILICQREYRNFSCEKIANEC
                     NVSRATLLRLCRKIGLDSFSDLKYLLKNNQLEFVNNDNNFHDVCENYRLLMNELQKIS
                     FQDICKIIYDAETIYIYGTGNEQKSLAQEIKRIFLSVGKCVIDLFDIGEIEFMRESFQ
                     KSDLFIIISLSGETKAGIEIAKFINNHIHTISLTRLDNNTIATLCEYNLYVETQKLDT
                     LQAISYEVVGAFYALLDLLHMNYIEYRRRKE"
     gene            complement(36403..37008)
                     /locus_tag="PC414_000635"
     CDS             complement(36403..37008)
                     /locus_tag="PC414_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2463047.1"
                     /translation="MKIKENVLMLDSTRGSNCFIVFDKEIILIDTGLPFMGKNIIKEL
                     KTLGIKLTDIKHILLTHHDVDHISNIKLLKEKTGAKVWAHVEDIPFIIGEKDRPGFKK
                     FIGKAISRRLIKDVEPYGENMQIGNIKIIHTPGHTPGHVCMIFEDVLFAGDLVKNKNG
                     NIVPYPNPWNWDYKKMMKSIRELDNLKYEWICMSHGTPCIK"
     gene            37187..37825
                     /locus_tag="PC414_000636"
     CDS             37187..37825
                     /locus_tag="PC414_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2463048.1"
                     /translation="MSELSKRDKEKIQRENEIIDKAEKLFCLNGFDNTTMNELAKEVE
                     YTKRTIYKYFSCKEDLFFAVVLRGYKRLWDNVKIESAKGKTGFEKIKLSYFAFHKFYS
                     VEPSLLCLMGMIGIVNSEKSDTDMLFKEKFFSFNKFMFDEIQGMFEIGKNDRSIRHDI
                     EIPTLMYSSIFTLTGFFNLLSVTGKSYLNNFNIDEEKFIETTLALLIDSIKA"
     gene            37957..38619
                     /locus_tag="PC414_000637"
     CDS             37957..38619
                     /locus_tag="PC414_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2463049.1"
                     /translation="MAKILLLEDNDTITFGIKTSLEKKNHTVCCYNTIAEAKKNFDKT
                     IDLILLDLNLPDGIGYEFCQYVKSIEDTPVIFLTVRDDEKDIIRGLDMGADDYVVKPF
                     LLSVLQSRINAVLRRTHSNTNNTITCENISLNKVEVKAYLDNVEVPLTAGEYKLLLSL
                     MENKNRAMTRTKLLEMLWDVDGNFVNDNTLTVTIKRLREKLKNPPCIKTLRGIGYRME
                     DN"
     gene            38619..39800
                     /locus_tag="PC414_000638"
     CDS             38619..39800
                     /locus_tag="PC414_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2463050.1"
                     /translation="MKTPRNINSTVVFIIFCLIVTFLLSVILTTITSFNEYNVLSKAL
                     GASYEYNPKSTESILKSLKNSSDKDFKTGNEILSKHGYSKSTFWSKNFFYFLVISFGI
                     ISTMATVVYIIKHRTNKQKLSRINNLTQYLEEVNLGKESVLLRCEDEFSHLEDEIYKT
                     VGELRCSKEFALKERQTLSDNLADIAHQLKTPITSMSLMAQLLSENCPDEEIDYINRL
                     TNQISRLERLVSSLLTLSKLDASTLIFESKLIDVHSLLTYAIEPIEGSLREKHQTFTI
                     EASPSVEFEIDINWTLEALLNILKNCSEHIEDGGYIVAYYSQNPLYVEIIIEDNGKGF
                     AQEELPYIFNRFYRGKNASKDSIGIGLALSKSIIERQNGTLHAENCHNGGARFIIKFY
                     T"
     gene            complement(39981..42641)
                     /locus_tag="PC414_000639"
     CDS             complement(39981..42641)
                     /locus_tag="PC414_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2463051.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIKLSIEKEGNWHGELFDYTYGSDLPLIENFSSVDDVMVKGQWYVGKINDEKRDYI
                     IYRNATSEYWNSMPEKDTIIEGRRPNNANEIALSKQYFENNPSVKIGDKITLPIGDRI
                     LNGKPLEPIAPKSKGESFKKISEATLTIVGKVDVSTSSVIPAYTALGYLDKTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKRS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYVNSVNYHTDVPEVVFTYGIPAFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTCFLHIIANQEGAKAVYGSDNYKDQRDIAVYLENSEPVE
                     SAFIEKLDNTEGVKSSLYYQKMSASTWLTEKDASKELAKNGGFKNIASSKKYSPIERD
                     GKFRIISNIIGLDDKSFTEYCRQISVDPKLFYDTKNPTSIVYNKEEDINRSTRRNKVY
                     IDYLNLSIGDNIKLNEKVYDEDKGDYTYNMKVGAITSTLPKIVDTSSKYTLMQIVPMS
                     MVQEISKNYDEVSRLRSNRLIGVFKVNNREDIPRVREKLESIYNKDYGSGDYSIEDVL
                     ESEKQKASGIKTMNLIVGFITGLLAMIGLSNVLATVSGNIRSRRQEFAMLRSVGLSPE
                     GIKKMLILEGLFLGITPLLLSIPVQIGIVYAFLRINEIYFIEYLPFAPISTIIGFTIL
                     ILFIVIASYMTGYKQLKNENIVESIKNETI"
     gene            complement(42638..43321)
                     /locus_tag="PC414_000640"
     CDS             complement(42638..43321)
                     /locus_tag="PC414_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2463052.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHLLGGVDRPTSGKIYVEDTEISSLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
     gene            complement(43665..44618)
                     /gene="blaCDD"
                     /locus_tag="PC414_000641"
     CDS             complement(43665..44618)
                     /gene="blaCDD"
                     /locus_tag="PC414_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HDF2463053.1"
                     /translation="MKRKKNFIWIAILLVGVVMVMYYVGKKHNDINQKTDKNYIKSEL
                     NKSELNKSKIENNNKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETR
                     RSPCSTFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYY
                     KKLIDKVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQ
                     TMAKIFEGDTNFKKEHINILRDIMKIDVNDKNINVYGKTGTGFDEKNKRVDAWFVGML
                     EREGDTYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
     gene            complement(45159..47273)
                     /locus_tag="PC414_000642"
     CDS             complement(45159..47273)
                     /locus_tag="PC414_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HDF2463054.1"
                     /translation="MSENKQVVNCDIIASNSKHNMCEYDAELLTFFPDEIAHLIEINN
                     KLDNAFKKAENLVDKLDKDYMDAKMYMVKNRGEIDPHEMFQNEQGLKQIDNYGAFMVK
                     VRDKINKIKDSPYFARIDFRLKDMDDESKYYIGRFAFDYEDELIILDWRSPIASMFYD
                     YEIGKAGYDAPIGWVDGEITRKRQFKIKNGKLEYALESSINIQDDILQKELSHTSDEK
                     MKSIISTIQKEQNQIIRNDKADTLIIQGVAGSGKTSIALHRIAFLLYRFKDKISANNV
                     IILSPNKVFGDYISNVLPELGEEPLCELSFENIAEVQLDRVINFESEKDPLEINDAKW
                     SERVRFKSTLDFVKLIDDYIKQMPNKIFIPKDYTFGSFTAKSDWIQSRFEAYNRYPVK
                     KRLEKVAEDIHYKFESDNIMEEDLPKVKSILKSLNGMLTIKNTLTLYKDFFKQMNISN
                     MFVMATKKTLEWSDVYPFIYIHAAYEGIQEDKIIRHVVIDEMQDYTPIQYAVINLLFK
                     CKKTILGDFGQLVNPNHTHTLDDMKQLYNDGELVMLNKSYRSTFEIINFAKKIQDISS
                     LEPIERHGEEPALLKCNNEQDEINKIKIEIEEFKKSDNATLGIILKTDNDAKAVYDAL
                     REKYSVHLISSESSSFTKGVSITSIKMSKGLEFDEVIVPSVNNKTYYSDYDRSLLYIA
                     CTRAMHKLKLTYTGELTQLIDM"
     gene            complement(47531..48391)
                     /locus_tag="PC414_000643"
     CDS             complement(47531..48391)
                     /locus_tag="PC414_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2463055.1"
                     /translation="MSYKVINVITAIDYIEEHLSEKLDLDIVANAVHYSKYHLHRTFT
                     TSVGLTMHDYIKRRKLTEAAKLLVFSKKPIIEIALIAGYESQQAFTSIFKAMYKKSPN
                     KYRKEQEFYPLQLRFVLKKDNLFSKNVLELEKEIKLASMSDIPLWMNLVRLVIDGFPN
                     LQEEEYIYQLEQYILEERALILKLNNVAIANMVFNRETRSIDFFGIHPQYRNSDIAQV
                     FLKKVIEDFLIDTDISITTFREGDKADTGHRDMIKKLGFAEAELLVEFGYPTQKFILP
                     CVEERDNRQN"
     gene            complement(48986..49348)
                     /locus_tag="PC414_000644"
     CDS             complement(48986..49348)
                     /locus_tag="PC414_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF2463056.1"
                     /translation="MKIYDILTRFYIQDIEKAIPFYENLLKEKCSLRFSYKEVGLELA
                     QIGNVLLLSGSDDALKPFIETKSTFMVDSVDEWRSYLLDNGAVVVRDKKKVPTGYNMT
                     LRHPDGTIIEYVQHTKQD"
     gene            complement(49582..50046)
                     /locus_tag="PC414_000645"
     CDS             complement(49582..50046)
                     /locus_tag="PC414_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HDF2463057.1"
                     /translation="MTRISNITIAEQSEYCFLAIRKTIDFMVEFSEFSKQSFEKISKY
                     LEERGILSSGAPIVCFHNMDLAKLDVEVGFPVATYIDGKNEILQRIVPAQKIITAIDL
                     GPYELQDPTLEDLFSWANSNGYKLHGDIYYQYLNDINRPASEYLTKMMLPII"
     gene            complement(50475..51896)
                     /gene="nhaC"
                     /locus_tag="PC414_000646"
     CDS             complement(50475..51896)
                     /gene="nhaC"
                     /locus_tag="PC414_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDF2463058.1"
                     /translation="MKERECRKASLFDAMIPITCLILFLSLGVLVYGSSPHVPLIGAA
                     AVAGLVAVYRLGFKWKELELSMFNSIKMAMQAILIIIIIGVLIGTWIVSGVVPCMIYW
                     GLKILSPNIFLVASTLVCAIVSLATGSSWSTMGTVGVALLGIGQSLGMPIGLIVGSII
                     SGAYFGDKLSPLSETTNLAPAMAGTDLFTHIKYMLYSTIPSLLICLVIYGVIGMKYSG
                     QALDIKQIELIRSTLDSTFNTLSPVLLLAPAIVIGLVVFKVPAIPGLIVGAVLGVLFA
                     VVFQGESMNTILDAAQNGYVSSTQIQEVDALLSKGGIMNMMSTVSLTICALSLGGILE
                     KTGMLEVVASSLLRLAKGVFGTVLCTMVTCTITNIIAGEQYLSIVIPGRMYNKEYKKR
                     GIHPKMLSRALEDSGTLTSPLVPWNTCGAYITATLGVSALTYGPYALLNIINPIVSLV
                     LIACKFKIARIEDEPETCLNFDV"
     gene            complement(51926..52990)
                     /locus_tag="PC414_000647"
     CDS             complement(51926..52990)
                     /locus_tag="PC414_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/ornithine racemase family PLP-dependent
                     enzyme"
                     /protein_id="HDF2463059.1"
                     /translation="MYPRLEIDIEKLKHNAKLISQMCHERNIKISFVTKSFCAQKEIV
                     EEICSEGIDHIADSRIQNLKNLQDINLPKILIRIPMLSELEEVINYCDISFNSELGTI
                     KKLNELCESKNIIHKIVLMFDLGDLREGYFYEEDFFSNVREIVRLKNIEIIGIATNLT
                     CYGAIIPSRENIGRLVSIAKRMEEKFGINLEIVSGGNSSSIHLLINNNMPEGITNLRI
                     GESILLGRETAYGENINGTYQDAFKLICQVVECKEKPSVPIGEIGVDAFRNKPVYEDK
                     GILKRAIIAIGKQDINIDSLIPIDTDIKILGASSDHMILDVSNTKYDYKLGDNLEFLL
                     TYGGIMSSSTSKYVSKKIIV"
     gene            complement(52975..54384)
                     /locus_tag="PC414_000648"
     CDS             complement(52975..54384)
                     /locus_tag="PC414_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HDF2463060.1"
                     /translation="MENSKVSLKIDVLVAEIGSTTTVVNAFHDINTNNPIFLGQGQAP
                     TTVFEGGDVRNGLSGAIKDLANKLSVDDIEYNDMFATSSAAGGLKMTVHGLVYDMTVK
                     AAKEAALGAGAVIRQITSGRIKRTDLNKIKEINPNIILIAGGVDYGERDTAIYNAEMI
                     ASMNLGIPVIYAGNVENQEEIRLIFEDTNYKLYITENVYPKIDLLNIEPTRRIIQSVF
                     EEHITTAPGMKYIKEMVNENITPTPGAVMEASKLLYKNIGDLLTLDVGGATTDVHSVT
                     DGSDYINKILVNPEPTAKRSVEGDLGVYVNMKNIVEVIGKENLQSELSIDIDAVIENY
                     PPIPKSKEEILFVERLTKEAVVKAMLRHSGKIRNIYGTTGKVKIAEGKDLTEVRYIVG
                     TGGALTRLPSRIEILDNMLKYNKNNELLFPKEKTKILIDNDYIMASMGVLSKKYEEAS
                     LKLLLKSLNFEEERLCILG"
     gene            complement(54384..56588)
                     /locus_tag="PC414_000649"
     CDS             complement(54384..56588)
                     /locus_tag="PC414_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892628.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HDF2463061.1"
                     /translation="MLKENKKLDIDYILKDLDKYKPKRRGWVWREHLENLEMGPFKYK
                     DCTKPLKKSVGLPSSKYFNNIDPQPSPVITTEIASGRFEDDIRRMRMAAHHGADHLMV
                     IRTAGQSHFDGLIEGTPQGIGGVPITRKQVRAQRKALDFIEEEVGRPINYHSYVSGVA
                     GPEVAVMFAEEGVNGAHQDPQYNVLYRNINMVRSFVDACEAKKIMAFANIAQIDGAHN
                     ANATAREAWKVMPELMVQHALNSIFSEKIGIDKSNICLSTVPPTAPPAPCLKIDLPYA
                     VALRELFSDYKMRAQMNTKYMESSTREATVTHVLNLLTSVLTRADIQSTITPDEGRNV
                     PWHIYNIEACDTAKQALVGMDGLMDMIELKDVGELRDKARELKERAVLYMEEISEVGG
                     YFESVEQGFFVDSGNYPERNGDGISRKIKGGVGEGTVYEREEDYLAPVTAHFGYNNVA
                     QYDENAIDNPSMLIDGCTFENPDKIIYIDELDDFDNVENRLKESYEYRNGTKIKPEME
                     WCADGIVMITMMLPTDKRTAEFAALEFVKKMNLQEIEVISREVMHESEGTRIEVKGRV
                     PFDIDLNNLVIPEEPKVLTDEEIRADIEEKPMKIVSATVGEDEHSVGLREIIDIKHGG
                     IEKYGIECHYLGTSVPVEKLVDAAIELNADAILASTIISHDDIHYKNMKKLHDYCVEK
                     GIRDKVMIACGGTQVTPEIAVEQGIDAGFGRNSKGIHVATFLVEKRREMSNK"
     gene            complement(56582..56956)
                     /locus_tag="PC414_000650"
     CDS             complement(56582..56956)
                     /locus_tag="PC414_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine aminomutase subunit alpha"
                     /protein_id="HDF2463062.1"
                     /translation="MKKREDDFEVRRKHLQELSDDELKERFWSLATQIVEPMIELGKK
                     NTTPSIERSVLLRMGFSSLEVKPILEGVMERGLIGKGAGHVVYKLAKSKNITVREAGL
                     LLVKGEYWDEVTCLFREGVESC"
     gene            complement(57034..58464)
                     /locus_tag="PC414_000651"
     CDS             complement(57034..58464)
                     /locus_tag="PC414_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent lyase/thiolase"
                     /protein_id="HDF2463063.1"
                     /translation="MNNSTSMKDMSYQAVMGRNNEIMKNAIGLDYSYFEQEGIGFDYE
                     KMMSETGYTLQDIEAIQSQYAVGNTPLIELKNLTKLARKCAKEGKGARIFVKDEAMNA
                     SGSFKARRAATAVYHAKQMGYKGVIAATSGNYGAAVASQAAMQGLKCIIVQECYDSNG
                     VGQPEIIEKARKCEAYGAEVVQLTVGPELFYTFLVLLEETGYFNASLYSPFGIAGVET
                     LGYELAIQFREKYKKDPDIVVCTNAGGGNLTGTARGLIKAGSINTKVVGASVDLKGLH
                     MASDNQFNKKSFTTGHTGFGIPYCTWPDRSDVPRSAARPLRYMDRYVLVKQGEVFYTT
                     ELLAQLEGIERGPAGNTSLAAAFSLAQELDEDKVIVVQETEYTGAGKHICPQLTFARE
                     NGIDIKFGNPREEIAGVNLILPERPELLKCVDIDMNKIRKSFIKNCISNNHIDNIDKL
                     SNRDIEFLMEEVKSSRDFVVDVLNNL"
     gene            complement(58457..58765)
                     /locus_tag="PC414_000652"
     CDS             complement(58457..58765)
                     /locus_tag="PC414_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-ketopentanoate thiolase"
                     /protein_id="HDF2463064.1"
                     /translation="MDAKINDWVIIHNIVLTPEERAPQVPEDTKKVSLEMWVKGFIQS
                     DASIGDLVEVKTITGRLVKGNLLKINPYYTHDYGKCIPELLQIGIQAKEILFGGVYNE
                     "
     gene            complement(58909..59976)
                     /locus_tag="PC414_000653"
     CDS             complement(58909..59976)
                     /locus_tag="PC414_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-binding protein"
                     /protein_id="HDF2463065.1"
                     /translation="MRKVRVGIWGFGAMGIGMANMILKKEGIEIVSVCSRSTSGKSMY
                     NVLGIERGERPEVIINKNYEEVFREKSVDVVLLATDSFTKKAFDKIIFLLNRKINVIS
                     TAEQMAYPQADDADLAKKMDEVAKENGVSILGTGINPGFVLDLLVLALSGTCEEVTSI
                     KAKRVNDLSPFGKSVMEEQGVGVTREEFIKGVEDKTIAGHVGFVESINMIADGLGWKL
                     DKIEQTKEPIMTIVDRKSKYGEALAGNVAGCRQCGYGYVNGEVLIEMEHPQQIIPEAE
                     GIKTGDYVSIKGIPNIDLQINPEIPGGVGTYAMIVNSIPLIINARPGLKTMLDIPVPR
                     AIMGDIRNQIEVELEEESKAN"
     gene            complement(60232..61605)
                     /locus_tag="PC414_000654"
     CDS             complement(60232..61605)
                     /locus_tag="PC414_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2463066.1"
                     /translation="MNEILENIDEGVHFVNKDGKTIIYNNSVQKMEKMNRHDILTNSF
                     FDIVNKMKINRSTLLEVLEKREVIKDNIQKYLDKNGREITAINTTIPIDIKGEFVGAL
                     EISKNMTTIENLLNRNYKKNLVNKVSIRQKKGYEFDDILGESNLIKEAIAKSKKACKS
                     DASVFIYGETGTGKELFSQSIHYGSNRKNYPFIAQNCAALPESLFEGILFGTSKGGFT
                     GAIDRPGLFEQANKGTLLLDEINSMPIMLQAKLLRVLQEGYVRRVGGTKDIPIDVRII
                     ATTNESPKVILNEHKMRKDLYYRLNIIHIKIPPLRDRKEDIPLLCKKFISKYNKKLNK
                     KVVGISDEAIKILKNYDWQGNIRELENIIYCTMSMMEDEVQIETSMINLNDYYNLKEN
                     KESNNYSLNDLEDKTLSKIISEIEEKYIYESLEKASYNITKAASILGMNRQNLQYKIK
                     KYNIKIL"
BASE COUNT        20754 a        10451 c         7269 g        23249 t
ORIGIN      
        1 acttgctact gttggcatag ttagtgttgt tgcatcttgt gttggctgag ttacacttgt
       61 tattttatcc gcctcagctt gtacttctgc ttgtatttcc ccatctgttg atttggctgg
      121 tactactaca tctatttctc ctgtatctgc tgttttacca cttgattctt gtgttactgt
      181 aaataccaat gttactgttt cctctgtatt tggaggtatt attgttccat cttcctttat
      241 aacactctta ttgcttgatg tttttattgc tatagtatat ccacttgata ctgatggcat
      301 agttaattta gttgcatctt ttgctggtgc aggaacgctt gttatttttg ccacttcatt
      361 gtctaatgct gcttgtaatt cttcatctgt tgatttagct ggtactgtta cagataattc
      421 ttttgtatct gctgttttac cacttgatgt atgtgttacc gtaaatacta attttactgt
      481 tgttgctgta tttggaggta ttattgttcc atcttttttt ataacactct cattacttga
      541 tgtttttatt gctatagtat atccacttga tactgatggc atagttaatc tagttgcatc
      601 ttttgctggt gtaggcacgc ttgttatttt tgctgcttca ttatttactg atgtttgatt
      661 agaagaactt cctcctgaag atgagcctcc tccatgtgaa gaacctccac ttgatgaact
      721 tcctcctgaa gagttagaac ttggactatt tccacttatt gttggagaat tatctccctt
      781 aacactactt attgtacctt tattatctat agtagtccct ttagaattat ttgtaactgt
      841 agttatctta ccaccattag tcacttttac ttgacttgct ccagatgcta ctgttaaact
      901 accaatctca cctttagaat tattttctat cttggctcca tttttatcat taacctttaa
      961 gttagttatt tttccataat taacaaaagt tccatctttt acatcatcta taactacatc
     1021 atccactttt gcatagttat ttacatcacc attaggcatg ttgatagtca cagtctttgt
     1081 atatgtacca tttaagttta ctgttactgc tttatcagtt tgtattgtaa atgcttcttt
     1141 aacctcacta gttggcttaa agtttataac atcattagca gtagctttat ctatagctga
     1201 ttttaattca gaaattgttt tagcttcaac tgtttttact tcttgtaatg acttcatttc
     1261 attaaatgct gtctcattac cattcccacc aactttagtt atcttatcaa aactcttaga
     1321 gttaactaga gatttttgtg aagctgataa gttctttcca actaaaacta ctggtgattg
     1381 atttttagca cctaatacac ctactgctag tgcatctata agttggtctt gtttattcat
     1441 accatcttta gttacataag cattttttaa atctgtcttt ttataaaatt cttttattac
     1501 ttttgcattt gtatcatttc tatctgttcc acctagtctt gtactatttg gtagtttatt
     1561 tttaacagct tcagaaattg aactctctcc acctactaca taagattgag ttattccagc
     1621 atcctttata aatttgtctg caactgctgt tccattttta gaatcagcaa gtattactgg
     1681 catattgttt tgtgcagctg gtgctcctac acttacagca tctgcaagtc ctttttcacc
     1741 attaactaca gctattttct taacatcact tatagcatct aatttctttg ctaattctaa
     1801 ggatgtttga tatctctcag ctccagagat tctctcaaaa gatattttta aatcttttat
     1861 ttctttttct atatttgtac ttaatacaga agttccacct attaagtata cttttttagc
     1921 ttttaatctt tgtatttctg ctttcgtctt atcatttaat ttatctttac ttgttaaaag
     1981 tattggagca ttttttgctt ttgcaaatgg tgttgcagat agagcatctg ctatagatga
     2041 gtcgtttacg attactacct catcagcatt tttccatcca tcttgactta ttttaacagc
     2101 agtttcatat ctttcttgcc ctgttaaatt tgttgccaca gtttgtgcac tagctatgcc
     2161 tgctgttgaa cttccagcta tcattgtaac tgtaagcatt gcagcaatct tctttggtat
     2221 tttcatttta ttttcttccc ccttgtccat ttgatattac ttaatttttt aactttgttc
     2281 ccgttctaat tctaaaggat acttaatatc taattttaaa gaggtttcta actataatga
     2341 tttcataatt aatttgtaat tattttgtta cattgcagtc attatgtaag gaataaagaa
     2401 attttcttat tgattgaaaa atttgtaaat ttatatttta aggtaagttt gatttttatg
     2461 ttaatgaatt gttataaaaa atagggtttt gtataaaaaa agtagccttt agaattaaaa
     2521 gtgctactct ttttacaaat aaattttatt atttatactt gtaattatat attaaataac
     2581 caattttatc catatgaaaa acaaataatt gcgatatttt tttaattatt ctttatattt
     2641 acattttaat tcaaattact tttgtaaaga ttatatctgt tgcatatact actttaagtg
     2701 ctaatatctc tccacctcaa ttagaatcct cgttatctca atagacgttt tctactgtca
     2761 aagttaccaa catttgaaac ttattaaatt tttatataaa aaagacctag aatataaatt
     2821 ccaagtcatt ttttattaaa tttatttttt acaatataat attgtcatga ttaaagaaca
     2881 caaatcatac acaaattggt cataaatctt acataaatca tctacaaaaa atacattatt
     2941 tatattaaat ctatataact aaattaagag catattgtac aatatgctct tagaaaagat
     3001 atttcttgtt tatagtttat agtatttctc tatgattttt atttttatca tttcaaaaat
     3061 aaaaattaag attacagtta ttatcatatt ttgtgtacta ttttctataa tattataatg
     3121 aaacaatgaa aaaacaataa ttaatgaaat tacgtttata gccccagaaa tcaaaaatgc
     3181 ctttttctta ttattcatat attccctctc cagattatct aaattagtct atatcaagca
     3241 tttttaagta cttatcatta actttatata tatttttttc tttagacaaa atctctatct
     3301 cttcaagatt attaaacact tccatcaact ggtcaactaa ttcaatttcc tttattccag
     3361 cctttaagta tctactttta ttctttttta tataattttc taatgtcata gaatagttat
     3421 ctttattgca aatatatttt aatatatgtt cttctgttgc tgatagagtt atgtcatttt
     3481 caaacaattc ttcttttaat atactttcta aagattttct atcatgcata ttacgcatct
     3541 cattaataga ttgtattata gacttgagca taaattctat aaatacagtt aaattatttc
     3601 ctttgttttc tacatcaagt atagccttat aatacttagt tctgttttta gatatcatat
     3661 atgatattga aaaatccttg aaagtatcat atcctttatc aatcaaatat agatatgata
     3721 aggctcttga tgttcttcca ttaccatcac taaaaggatg aacatataca aagtaaaaat
     3781 gaattatagc acttttaatt aaattactta ctttgctatt ttccataaaa tctattaatt
     3841 tactaataaa gtcatatatc ttcaaaggtt caagccctgt atgtataatc tcaccttttg
     3901 aatcacatac atatactttt tcagttctat actcatattc ttctgaatcc aagcagtttt
     3961 cgcccaatat atgatgcaaa tcatatataa atttgtgact atacaattca tctaagttat
     4021 ctaatccata ttctaaggct ctatgattat tgtaaatcat atattcactt ttattacttg
     4081 gtttcatctt tcctctaatt atagattttg caatctttct agttgaaaaa gcaccttcta
     4141 ttacactaga ataataagac tctagtgcca aatcattttt atcttctatt gttttaaata
     4201 tcttacttga tgttaattta tctatttcat ttattaaatt taatatattt tcagtttcat
     4261 gatagtatag ttctttatta tcaaaactat ttatagaaag tacgatttta tttccagtaa
     4321 agtatttgtt tttgtattct tctattgtca gtttaatcac caccttagat tatctttatc
     4381 tattaatatt aacaaaaagt attaattttt acactatttt tgattatttg ttttataatt
     4441 atcaaactca atttagcgtt aaagcataat aaattttaat aaataatgca aaaatttaag
     4501 aataagcatt taaaatatat gttacaggaa gtttacaact attatttata atacattagt
     4561 tatatattat aatatctcga caatgtctta acaatatctc gattatatat tttaatatgt
     4621 aactaatatg ttataatatt ttaattaaat taaataaatt tatatttttt tgaaattact
     4681 taatatttta tataaaaatg aaaaagcacc tcattctgat taaaaatctt tctgagatac
     4741 tttttaaaat attaaatact acatatagct tacataagtt taaaatttaa tctattataa
     4801 aattacccta attgccaaac tttttaatat aaattttcca acatcctaca aatataatca
     4861 atagatatag aatcctaact tacaccaata tatatttatt taaaatagtc ctaaaatacc
     4921 tgtaatagct cccactacta tcagtataaa cattattttt attggagaaa gacctttttt
     4981 taatgcaaag agtactaata aagtaagtac caaaggaatt aatcctggaa atatcttatc
     5041 aagtaaatcc gtttgaagac taaatttaaa atcaccacta gtataactta ttggtgtaga
     5101 taaattgata aacctaccta caagcccccc catgactaag gtacctagta tagaagcccc
     5161 ttctataacc ttatttacta ttccaccttg aagtatactt tcaacagctt ttttcccata
     5221 cttatatcca tttgtccaca ttccaaaaga aattgaagtc attattatat attgagctat
     5281 tacaaataat ataggtcctg caattgatgc tcctccctca gttaaaccaa tacaaacagc
     5341 taacactata ggagttataa ctccttgagt taaagtatct ccaattcccg ctagtggacc
     5401 catcaagcct gttttgatag cattcatacc atctccactt atttcatctg ctccattagc
     5461 tttttcttct tccatagcta ttgttattcc atgtataaca catccacata taggttctgt
     5521 gttaaaaaat gctagatgag tctccacttc ttgttttaac tcttccttat ctggatataa
     5581 ttttttaagt actggcaaca ttgaatgtga aaaagctgtt gattgcaatc tttcataatt
     5641 gtaattagat tgtgcaaaga aaaaccatct taaccatgat tttattacat ctttcttgct
     5701 taacgttttc ttagatactt cattttttat atctgacata atattttccc cctcacatgt
     5761 atttaagatg tagcactatc ttttccgtac ttaattgaag taattgtaag agcaagtatt
     5821 actccaataa cagatattac tattatatct actttaaaat aaacagccat tagtattcct
     5881 aatatgaaaa agggcatagt aaacttattt ccaatcaatt ttaaattcat agcaatccca
     5941 agtgctggca acatagcccc aattacatta aatacatgta acacattatc attcattact
     6001 tccaacatac catctatcgc atttggacca aaataaacaa ctaataatgc tggtataaaa
     6061 ctcattgcaa ataacagtat ctgtggtggt actatattta taaatgctac tctatcaaca
     6121 ttcccttttt gaacctctct atcagcccaa tgtgcaaaaa aagaatttac tgtcatcttt
     6181 ccaatccaaa gtacagttgc aacagttccc aatggtacag ctattgccat tgcagtactt
     6241 acatctagct tagatgctat agctaaagca gtacctacat atcctgcaaa agctgggtcc
     6301 cccatctgag ctccccctgc tgatataaaa cctagatata tcatatttat acctgctcct
     6361 ataattgctc cttgaacagg gtctcctaat ataattcccg ttacaaatcc agccaataga
     6421 ggtctattcc atgtaaaaaa tccaacataa gtcaaccacg gactccaaga aagataataa
     6481 aaaattgcta ttaatactgc ttgaattagt gaaatgtgca tattcttcct ccctattttc
     6541 ttttaaagat ttacaagata gtcttcttta caataatgtt cctacatcaa ttttctttgc
     6601 atctggtact atttgtataa agcatttaac atcttttgat attagctctc taaatatttc
     6661 tttttcttca tctgacacag atatattttt ataaaaactt tttctaccaa gccctgctcc
     6721 catacctcca acattcaatt cctttaaatc taccccacta tttactaaac ctaagacagt
     6781 tttagggtat ttgaccaata ctattaaatt ttcatctcca ttatgtgatt ttaagacttc
     6841 agcacctgat tctacatcat gtacttcaac cttaacagtt gatggagctg ccatttgtaa
     6901 aacttgaact ataaatgggt cctttactac taaatcatcc acgattacta tcctttttgc
     6961 tgatgtaatc ttacaccaag cagtaataac ttgcccatgt attaatctat catcaattct
     7021 tgttaatgct atattactca taattattcc tccagatttt taatttattt tacaacttta
     7081 aaacagtttt aacatctttg attcctgata tcccaacttc ctcacataaa ttcttcaagt
     7141 cttttaactt catagtatct ctgtttaaaa ataactctaa aagcataggt aaatttacac
     7201 ctgtcaaaca ttctaactca cataccttag caaatttatt tattacaata gcagatgtat
     7261 taaatggagt tccgccataa aaatcaagca atactaaaac cccttcatcc ttatatttct
     7321 caattccatc ttctaacttt ctactaaact catctatgct gtcaccatgg tttaatccta
     7381 aagtttctac attttcctgt ttgcctacta ttaattcagc actttctaac attgctttag
     7441 ccatatttcc atgtgtggct ataattattc ctgccatatg aatcatacct ttcttaacct
     7501 tttttatatg gattattatt tagtactcta atctaattat ctatattaat cattatctta
     7561 ccaaagaaaa tatccctttt gtatactttt tcaaaataag ctggtacttc ttctaaattt
     7621 attctatgtg ttactaattt ttcaacattc atatcaccct tttgcatatg atacaatcca
     7681 gcactccatt cttttcctgg gaatggaaag gagtttccaa accaagttcc ctttacagtt
     7741 aattcacttc ttactatctt ttcaaattgc tctctagtca aagctacatc cccatatggt
     7801 acacctgcat acaatactgt acctcccttt ttagctagca acagtacttg accacatgtc
     7861 agtggagtac cagccgattc aataacaata tctgcaccat ctccatcagt taaccttttt
     7921 atttcttcta caatattctt ttcttttgca tttatacaaa tatctgcccc taactctttt
     7981 gccaagtcta acttctcatc aaatacatcc acagctatta tttttgtaga accaaatatt
     8041 tttgcccatt gtattgaaaa taatcctatt ggacctgtac caagtactac aacagtatct
     8101 ccaactttag cttctgacct aaaaagacca tgtcctgcta tacatactgg ttctagcgca
     8161 gcggcagtct cataacttat ctcatctggt atttttatta aatttctctc tttcaccttt
     8221 gtatactctg caaaacaacc tccaagttct ttatttccta ttattgctac attattacat
     8281 cttgaataca agcctttttt acattcatca cattcaaaac atggcatagc tggacaaaca
     8341 gcaactctat ctcctatttt aaaactcctt acttctttac caacctgtgc cacttctcca
     8401 gaaaattcat gccctaaaat ttctccaacc atatgtggac ctgtctttga atattttgat
     8461 atatcagaac cacatattcc agctactttt actttaatta ttacatcatc tttttctaat
     8521 atttttggta catccacatc ctccactctt gtgtctctta taccatagaa tctaactgac
     8581 ttcactatta accaccccta ctttttattt gagaatatat ccctattgtt tatatgttaa
     8641 atatctaaag caactttcat gccaatatat gtaagcattg tattcatgca ttttataaaa
     8701 ttatcattaa atgtgtgtca aagtgtgtca aaagtttctt atattaaaac ttatgtgtgt
     8761 caatataaac gacactaaca ttttatatta gttctcttat tataagtttt ataaaatgta
     8821 ctttttttgt tatttttata aaattgttcg gaaatttccc ttctatttgt gatataatat
     8881 taaatataag gtatcaatat aaattttcat gcgaatatca gaactttttt gacactaatg
     8941 ccttattcaa tataataatt taactttttg gaggttgttt atgaaaaaag tgtatcaagg
     9001 aaagactaaa gatgtttttg aattagataa tggaaattac ttacttaaat ttaaagacga
     9061 tgtaactggt aaagatggag tatttgaccc aggcgaaaat agtgtaggtc taagtataga
     9121 tggaattggt agagctaatt tagagacatc tgttaagttc tttgaaattc taaacaatgc
     9181 tggaattaaa actcattatg taagtgctaa tttagaagaa gcaactatgg aagttctacc
     9241 agcaaaagtt tttggtaatg gattagaagt tatttgtcgt tatagagctg taggtagttt
     9301 cttacgtcgt tatggttcat gtgttgaaga aggtgctaaa ctagattgtt acgtagaagc
     9361 aactttaaaa gatgatgacc gttgtgaccc tcttataaca tctgaaggtt tagaagcttt
     9421 aaacattatg actcaagctc aatttgattc aatgaaaagc atgactcaaa aaatttctaa
     9481 catagttcgt gatactatag ctgaaaaagg tcttgagctt tatgatatta aatttgaatt
     9541 tggattctac aatgatgaag taatcttaat agatgaaata gcatctggaa atatgcgtgt
     9601 ttataaagat ggtgtaatag ttgaccctat ggatttaact gctcttttat tagataaata
     9661 gtatatacat ataatttaca aatataataa acttataaat aagataagct atgaagtaaa
     9721 gactttttaa atgttgatta tttttaaaca ttttctcgcc tttaatttca tagcttattt
     9781 aaattaatat actctaatta gtagataata gctttaaccc tacaattccc accacaataa
     9841 aacctataca tgcaattctt gatatactaa taggctcatt taaaatagcc atgcctacaa
     9901 tagctgtacc gacggtacca attccagtcc atattgcata tgaagttcca agtggaagat
     9961 gttttagtgc taatgacaaa aaataaaaac ttacaatcat tccaacaaca gtaagtatac
    10021 ttggagttaa tttagaaaat ccatgtgaat tttttagttg aaaagcccaa aacacttcaa
    10081 acaaacctgc aacaaacaaa aataaccatt tcataaattc atccctcttt tcaaatatgt
    10141 ctcttatttt tccaataaaa taacctgaga aatattataa aatatctccc aggtttttgt
    10201 ccttccgtgt tcacaacaaa aactatgttg tgggttttat ctcggaccag accaatcatt
    10261 aataattgcg gaaccctata aaactctatt aattattcta ttttatctat aataatacta
    10321 tcaacatata taaaaaaagt caatatatgg gttccatata taataaaaat aacttttata
    10381 taccttttaa tattcttatt cacttctctc catacccatt tgggtcaatc taagaatatc
    10441 taatttttat ttaatatgtt ataataacat aaaaatattg aaaggagtaa tttctatgaa
    10501 tttctataaa attgcagtat gtcagatgat tactacagaa aataaaattg aaaatatcaa
    10561 tcatgctgta gatatggtaa cagaagctgc catcaatgga gctaaaattg tagttttgcc
    10621 agaaatgttt aactgcccat atgaaaataa atattttcct aaatttgctg aagaatatcc
    10681 aggtgaaaca acaacgatat taagtaaatt agctgagaaa catggaattt atctagtttc
    10741 tggtagtatt cctgagttag aagatggaaa aatctataat acttgctatg tatttgataa
    10801 aaatggagct ctaattggaa aacatcgtaa aatgcactta tttgacattg aagttacagg
    10861 aaaagttagt tttaaagaat cagatactct tacagctgga aatgatgtca ctgtaataga
    10921 taccgaatat ggaaaagttg gtatagctat ttgctatgat attcgtttcc ctgagttatc
    10981 acgtttaatg gctcttaaag gtgctgaaat cgttatctta cctgctgcat ttaatatgac
    11041 tactggtccc gcccattggg agttatctat aagaatgcgt gctttagata atcagatatt
    11101 ctatgttggt gctgcacctg ctagaaatat gaatgcttct tatatagctt ttggaaattc
    11161 aagaatttca gacccttggg gaagaataat tgctcaagct gatgaaaaag aatgtattat
    11221 ttatgctgac attgatagag atttaattcc tgacataaga caacagttac cacttcttaa
    11281 gcacagaaga actgatttat atgaacttaa tactttgaaa taaaacagaa cataacccca
    11341 taaatcataa attaagtgtt aaaaaaattt atggggttct cattaatttg atttaaaatt
    11401 aatttcataa aaataatgct aatattaatt taattaaata atactaataa ttactaatga
    11461 ttatgctaat tatcagcatt tactaattta atcactatta gttatttctt ttattaaata
    11521 tctttacttt tttacttatt atgtcttcta gctttacatt tggattatgg tatttctttc
    11581 tatttttagt cacatctttc tattgaatta cttgcttaaa acaccaatgc acacatgcca
    11641 gataatgttc acaattattt agaaatcttg ctctaccttc ttcaatgttg atattatctc
    11701 tcctagttga tattatcttt cctaaaattt cttttaatta aaaaacaaca ttagttgata
    11761 tatcaagtat attttaagca actgaaaaaa acaataataa ttgatatatc aactaattaa
    11821 tgtagattaa ttcattttta taatttatat actttttaat taatctacct tatttaatta
    11881 aaatccttcc tttctattta tccattttca acttaaatta taagatttca tcagctatat
    11941 ttttgcatac caataattat taaatatcaa atatatattc tattaaaatt tctaataatt
    12001 tcatccttga tttatttaat atataaaaat atactaactt taatgattaa gttttgaaat
    12061 ataactaaga atttgataat ttattgagat ttttatacta aaatatacat actatacaaa
    12121 cttaatttag aagggagcat tcttatgaac aataaaatac tcgtcgtaga tgatgaggaa
    12181 ggtataataa cactcttaaa agattacttt gaaatgaatg gttatgaggt ttacactgct
    12241 caaagtggaa aagacgcatt aaaaaaaata tctaaaaatc cagatatcat tctacttgat
    12301 ataaacatgc ctgaaataga tggaattgaa gtttgtaata gaattagaga ttatgtatca
    12361 tgccctattc tatttcttac agctagaatt gaaaatactg acaagataaa tggttttcgt
    12421 gctggtgctg atgattatat tgtaaaacct tttgatttag acgaacttgg agctagagta
    12481 tctgcccatt taagacgtga acaaaggaag tcaaatcaaa ctaaaattaa attttataat
    12541 gatttaacaa tagattacga aaaaaggtta atttgtatat ataataatca gatttcactt
    12601 tcaaaaaagg aatttgatat agtagagctt ctctctatga atcctggaca agtatttgat
    12661 aaagaaaaaa tatatgatag agtctgggga tacaatagca atggaaatag tgatgttata
    12721 atggaacaca ttagaaaaat acgaatcaaa ctatcaaaac atactcttga aaattatata
    12781 gaaacagtct ggggagttgg ttataaatgg ataaaataaa taatatgtca ttaaaaaaag
    12841 ctttctttgt tcttacttta tcttcactaa ttatagcatc agtgcttact actttcgcat
    12901 acattttact taataatcta tataattcta tacaagataa gtatttaaaa acacctataa
    12961 atacaggtat tataaccata gttcaaaaca atgtgaacca atttagtgat tatgataaaa
    13021 cattacttaa tataatcaac atacttcagc ttgtacttcc acttatcttt tttataggac
    13081 ttttattatt agctgatata attttttaca aggtaaaatt aaaaacaccc atagaaatac
    13141 tcaataaagg tgcaatggaa atatccaaca acaatttaga cttttgctta gaatataata
    13201 ataatgatga attaggtaat ctatgtaatg cttttgaaaa aatgaggtct gaactaaata
    13261 aaaacaacat aaagatgtgg actatgatta atgatagaaa acaacttaat gctgcttttt
    13321 ctcatgattt aagaaatccc ctaacagttc taaaaggtta ctcaaactat ttaacaaaat
    13381 acataccaac aggtaagctt agtgatgaga agattttatc aaccacacaa cttatgtctg
    13441 agcatattaa cagaattgaa tactatgtag aaaatatgag taatgctcaa cgattagaag
    13501 atttagttgt ttcaaaatct atgtcgaata taaacaagtt tatagaaaac ttagatgaaa
    13561 atatatctat cattgctaaa caagaaggaa aatcttttac acttaaaagt caaataaata
    13621 atataaatct attttttgat gaaaatatca ttcttagagt cgtcgaaaat ataatatcga
    13681 atgcttttag atatgctaga aataatgtat ccatacttat atatcttgaa caagaactcc
    13741 ttacctttgt aattgaagat gatggtatag gatttagtaa ggaatctcta aaattagctt
    13801 taagaccttt ctatagagat aagactttaa atgacagcaa tgcacacttt ggtatgggtc
    13861 tttatatctc aaaaatatta tgcgaaaaac atggtggctc tatttctatt gaaaataatt
    13921 ctactaacag tgctaaaatt actgctaaat tctcaacaag aaattaaaat gattttgtaa
    13981 gctttcactg ttcttatata aaatttgtta gtttagaatt tataaaatgt atttcaattt
    14041 acaaccaata tatatactat tttgtagaag ttgtaagagt tgcttcaatt ataatatttg
    14101 gcaaaaggca tttgaaataa taatagaaat atatatttaa atatgtagaa tagtatgaaa
    14161 ttagaatttt ggaggtgatt atattaaaag ttttgctaat aatattacat tctctcgtat
    14221 tgtgtttgca ttgctaatgc ttagttctac attctcttca ctgcctttct ggatatgtta
    14281 tttttggtgt ggaattagtg atatacttga tggtcttatt gcccgaaagt tgagacaaca
    14341 aagtggaata ggtgcaaagt tagatagtat tgctgatttc atttttgcgg ttacaatttt
    14401 ggttactgtc ggaaaaaata ttgctcttcc catgtatcta tggtggtgta ttgttgcaat
    14461 agcattgtta cggttgatta gttacagtat tggttttttt aagtatcgca cattttcatc
    14521 gcttcatact ttgatgaata aaatcactgg aatttctatc tttgcttttc cattactcta
    14581 tatttgcttt ggtcttgatg ttgcaggaat tatgatatgt atcgtggctt ttctttcttc
    14641 ttttgaagaa atagcaatta tgataaaatc aaaagaatta aatcgtgata taaaaagtat
    14701 atttatgtga caacttagaa tttgtgacaa aaataattta tataataatg aggaagtaaa
    14761 acataaaaaa ttatccacaa gtttatgtgt aaaatagttt taaagcaata gagttgtatt
    14821 gtaaagcctt tagtgcataa attacatttt aaattaaaaa tgatactgaa actgcttatc
    14881 agcactgcga attatccgca aatagtgaag actttttaga tttaagctaa aagttgggaa
    14941 aaaattgagt tttaaaactt acactcttct tatatagaaa aggagagtgt tttttatgat
    15001 tataaaagca aaacaattat caaaaatata tggttctaat aacaataaag ttatagcact
    15061 taataatgta aatttagaaa taaactcagg tgaatttgtt tctgtaattg gaccttctgg
    15121 aagtggaaaa agcacacttt tgcatatttt aagtggatta gataatccta cctctggaca
    15181 agtgttactt gatgataaag atatatataa acacactgaa aaagaactat ctgcacttag
    15241 aagaaaatcc tttggattcg tctttcaaca atttaatctt ttacctgttc ttacagcatc
    15301 tgaaaatatt tcaatgcctg tacttctaga taaaaaacaa cctgataaag actatttaaa
    15361 tgaaatttca tcattacttg gaattgcaga tagactcaat catcttcccc atgaactttc
    15421 aggcggtcag caacaaagag ttgcaattgc tagagcactt attgcaaaac cagatataat
    15481 atttgctgat gagcctacag gaaacttgga cagtaaaagt ggcagtgaag ttatgaatct
    15541 tcttataaaa acttcaaaac agtttggaaa gactttagtg gtaattactc atgatgatag
    15601 aattgcaaaa cttgctgata gaaaaatttc aattattgat ggtgttctta tggaggtgaa
    15661 atagaaatga agaattacct ttcactttca ataaaagaat taaaaagcca gaaatttatg
    15721 actacattca tcattatagc aatagtacta tcaactatta tgacaactgt ggttggacag
    15781 tcaattggta tcctacaaaa tttaagaatt gaacaagccc gttcttttaa tggtgataga
    15841 catgtatcct ttcaccaact cactaaaaat caagttgatg acataaagaa agatgataga
    15901 gtttatcaag ctggtacttc aataactatt ggcagtagta aaataaagga ttctggtatc
    15961 tctgtacttg taaaagagta tgataaaaca ggactttcta actacccaaa attaatgaaa
    16021 ttaaaatctg gacatttgcc taaagataaa aacgaaatag ccttagatga aaacacacta
    16081 aagcttatgg gtataaaacc aagactagga gtgactatac ctatgaattt agatatttca
    16141 cttttaaatg ataccatacc accatataat tacacagcta actttaaatt gagtggcatt
    16201 ttagaggatg attatacagg atatgtaagt ggtatagtaa atggtattgt aggtaaaggt
    16261 acatcagaaa atctattacc taagagatat attttatcct ctttggattt taaaataaaa
    16321 cagcaagaaa agtttcaaga aatagtaaat caattagcta aaaaaataaa tctttctcac
    16381 aattcaattc aatataattg gatttactta aatgctctta agatacagtt tgaaaaagat
    16441 gaaaatagtt ctaatagtga tggtatatct atgattatat tggtatcttt atttgttgcc
    16501 ttattggttt tactggcttc tggacttgta atttataata ttttaaaaat ctctgttaca
    16561 aagaaaatta aagagtatgg atgtttaaga gcaataggag ctgaaccaaa ccagatatat
    16621 aagatagtta ttttacaaat cttaatttta tgtactgttg ccatacctat tggagctgta
    16681 attggaatta tatcttctaa aggcataact ggaatggtca ctaatatctt aaatccagat
    16741 atacttctag caaatgataa taaagaaatt actgagctta tacataaaaa tacaacagca
    16801 tatatattcc cattagtatt aagcactaat gtatctttga ttttttcatt tatttcagca
    16861 ttaccatcag caatatatgc ttcacatgtc tctcccaaaa ttgctatggt tggtagtact
    16921 accaaaatca aaagaaaaat taaaagagaa aaaacaataa aaaactttga aagacatcta
    16981 gcatggctaa acttaaaaag aaataaagga agaactataa taactatact atcattattt
    17041 atgagtataa cagtattcgt tgcactgagt gagttctcta atgtattaga tgtatctagg
    17101 tcagtaagta atttaaaaga aggagatttt tccttaacaa atgaaatatc tggttttgat
    17161 aaatcaacac tagataaaat agctaaaatg aaaaacgtca atcgcacttc ctttataaag
    17221 tactctgaat acaaacaggg tgaaatagat acagatataa attttgaaaa tagtggtgag
    17281 atgttaaaaa tcattggaat tgatgagcaa actttaaaag acttaatgcc ttctattaca
    17341 gattctatac tagaagactt taaaaatggt tctatctgtt ttataaaaaa tccgattgct
    17401 atttctacac caggtgtaaa aactagatat acaaatttaa aacctaaaga tagtatcaca
    17461 atcaacaaaa aacaactaga tatatatagt acagtagata aaatgttctt tttacaaggc
    17521 aatggctgga taaatggtgt tgatgttatt gtatatgatt ctgtttataa cactttgaca
    17581 aataaaaaca agttcaacca gataaatatt tatgctaaag ataaatcaaa attagaacaa
    17641 ataagattgt ctattgaaca gatttgtgaa aataatcctg gctctcattg gatatcatat
    17701 attgaatcag ataaacaact taaagagagt tttaaacaaa tagaatttct agcatgggca
    17761 gttatattat ttgtgggatt gattggtact ttaaacatca ttaacacaac tcatacaaat
    17821 ataaatacta ggactaatga gattggagtt aaaagagcta ttggtatgag taataggagc
    17881 ttatataaaa tgtttttatg ggaaggtgtt tattatggaa tttttgctgc tatatttggt
    17941 tcaattgctg ggtatgccag tgctattatc ataaacatgg caactataga aaagcttgat
    18001 ttcactaata taccaattac ttcaatttta caagctacta ttatttctgt acttgcatgt
    18061 attatagcta ctcttattcc gttaagaaaa gttaaaaaga tgaatataat tgactgtata
    18121 gatatcaatt tataactatt ttacagataa taatataaat taaattatca atgactgtct
    18181 ctattttctt tcaaatctaa ctaataaatc tttttaacta taatataagc tttaaaaaag
    18241 gcctgtctca aaatagagat aagccttttt tcattgtcga aataatgaaa atatatttaa
    18301 cttcacaaaa aaagagtgcc tcacgaatta aaaatttcgc tttgagacac tctcctttaa
    18361 tttaaaatat atagttaaat gctatgccaa aatttcacac atgctccacc tgattttgaa
    18421 ttttctattt taacagaacc tccaagctgt tccactaatt gtttgacaat gtaaagccca
    18481 agaccagaat gtccaccttt tgtctgccta gcttcatctc ctcgataaaa tttatcaaga
    18541 gcctttttta aatctttaga gctaaaccct ctaccagaat cacaaatctc ataagaaata
    18601 caattttttt catcctttac agtaatacta atattaccac ctgatggagt gtattgcaga
    18661 ctgttggata tgatattatc aaaaattcgt gtcaacctat caacgtcgat ttgaattggg
    18721 gattgtatat tcccctgcat tttcaatata agttcaactt ccttttgatg agcctgtaat
    18781 tcatagtctt gcaccttttg cctcaaaaat tcaggtaaat ttataggaac aagatttaat
    18841 tgtatatttg acttttctaa gtcagacgta tattgcatct tttggacaag agcagcactt
    18901 ttttctgtat tttctcttat cactgtaaga tacctatgaa gtttttcatt gtcatctgta
    18961 ttattaccaa taagtgcatc tgtatatccc aaaataatag aaagaggaga ttttaaatca
    19021 tgtgctagag cttccaccat ttcaacacgt tcctgctcca tcttccactg tgcagataaa
    19081 gagcctttta attcgtcttt catttcagaa aaggcactac aaagtttacc gagttcatta
    19141 tctgaataat agtcgatttc aaaatcaagg tccttttctt ttatcttctt ggacgcatca
    19201 atcaatagat gtaatggttg attgatattc tttacaaagc gcttagaaag ccatctagta
    19261 aaacccacga tatatagaaa tggagaaaac aaagccatta taataactgc aaatacaaat
    19321 ctccctctaa tatttgcaaa ggtgattttc acttggtaga atagaataac agcaccctca
    19381 atctttccat tatccccttt gattggaaca gtatggatat aaccaccttt acgaacagtt
    19441 ttattgataa aattattaaa taattcttct tttgttttaa atagtttttt ggggttagta
    19501 ccatataaaa tattgccatt attatccaca acttgatata gcatatcatc cccacgaatc
    19561 gttcttttta aaccttcttc attcgattgt gataacaaag ctatgttttt ttcattgatg
    19621 tatttttcaa ttctaggaac ttgtcgctca taatagtttg gtggatagat atccttattt
    19681 aaggaatgaa tatacaaata gagagcaaat acataagtaa tcactgtggc aactatactg
    19741 gctattataa taaaaataaa agtatgtcta aattgggtaa taagggattg atttttcatt
    19801 taataacctc tctctcttta cattttattc cacttatacc caatacccca aactgtggaa
    19861 atatattctt tttctggtgt cacagcagaa aacttagctc gtattttttt tactcgttca
    19921 acaacagttg aggaatcacc ttcagaatca cacccccata ctttttcata gatttgttct
    19981 cttgagaaaa cttgtcctgc atggagtgct agtaattcaa caatatcata ctctattttt
    20041 gttaaatcaa tattcttacc atcaatttta attgcacgtt ccttcatata taagcataac
    20101 ttcccaaaat acaaagtaga tctcttgttc tcttcattga tatactgtga acgcttttcc
    20161 ctacgcagat tagcttcaat ccttgccata agttcacgca gtccaaatgg ctttgttaca
    20221 taatcatccc ctccaaaggt caaccctttg attttgtcgg tttctgattg ctttgcactt
    20281 aaaaatataa ttgggcataa aacatcatct ctaattgcct gacatacctc aaatccattc
    20341 attttaggca tcataatatc cagtataata aggtcaggct cctgttttga taactctata
    20401 cctgcctgac catcataagc tatcaacact tcatgtcctt ttgccttcaa ttcatcctca
    20461 agtagcatta ctaggtccat ttcatcatca ataatcaata ttttactcaa tataacacca
    20521 cctttctctt atgggaaggt cagtattaaa acaatattcg tctactcata acttttcctt
    20581 ccctcccaca ttttaaacca aataacgcct cctatcaaga atataatcaa acctattgct
    20641 actactataa gctctatact agctgtttgc atcactttag agtaaaatgc ctcagttgaa
    20701 atagttccag cttctttaat aaaataaatt cctggaagca tacctagttt tactggccat
    20761 gtccatggaa caaatgccca tactttttca ccaacgctag ttaatccaag tattgctgcc
    20821 atcaaaattc caccaatact aataccaata gatgcaccca taccccatgc aaaactcacc
    20881 caaagatgaa tagctaatag tggaagtgtc ccaatcacca caagtcctgc tgctgacagg
    20941 aataaccata ttgttgctcc agatggcact gcaatactca ttcctatgca aagtatcaac
    21001 gtagcaataa atgtgcatat agttaggcaa aacagaagaa aaagaaattt tcccagatat
    21061 aatctgacac gagaaatacc tacacataaa aaaccattaa aatttccagc aagctcttcc
    21121 tcctgcacta taaatcctgc taaaatacct actccaatag gaatgatgaa tacagtccat
    21181 actgtaaaaa atccttcaaa agcaaattct tgtgtacttc cagatttagt tgtgagataa
    21241 aaaaccacac ataatgaaaa aataataggc atacaaagag taagccactg gacagctgtc
    21301 ctctttgctc gaagccattc tgatgataat agttttatca tttgtcatcc ctcctattga
    21361 accaagctgc agtaagtgct gttaatacta taaacacaac caaagaaaca acaatcccaa
    21421 taggaatcac tgaagtattc aaaagtgggt ctccagcttc taatatagta ccgttaggat
    21481 gtatgttgat gattgggcat accattctaa ctgcccaact ccaaggtact gcaatccaaa
    21541 atggttttgt cgcagcaaga acaccaaata tcatacctaa agctccaact cccatactta
    21601 ggaacatacc tttccatgta gctagccata gctgaagtgg aagtatagca agagaaacaa
    21661 cccagcatac aatacttgcc cctataatct gtccaaaagg aactggacca gctttcaata
    21721 ttaaaccagt tataattgtt acgactatta aaactagcgt tgaaataaaa ctgtagactg
    21781 ccatagctat aactttatta atccaaagtg tcataggaga cacattgtgt gtacgtaaag
    21841 cacgataatt tccagctttt ttttcctgga aagctaccaa agtagcgaac aatgcaaatc
    21901 caataggcag aaataaaaat gaccaccagt taaaaattaa tgctaaaaga ttttgccacg
    21961 cccaagaagt tgtttgagat aatggatttt gcatgagtgt tgattgcatt ataaatgcat
    22021 atgctgcaaa agacactggg aaaaaaacaa taagtttacc cataaatgtt cttttatact
    22081 ttaaaagttc tgattgtaaa gcattcatct gttttgtcct cctttaagaa taacatctgt
    22141 aaaaaattct tctagatttt cattagggtc aggcattcct tgaaataata gtcgtcctcc
    22201 atttataatt cctatattat ctacaacttg agcaacctct gatagaatat ggctagaaag
    22261 aattacagtt ataccttttt tgggaaaaga agcaatcaat tcacgcaatt cttgaatgcc
    22321 aaaggggtca agaccattgg ttggttcatc tagtattaac aactctgggt cactcaaaag
    22381 tgcaatagct attcctaacc tttgtttcat acccatagaa aattgtgaag ctctcttttt
    22441 acctgtattt ttcaaattta ctatttccaa cacttcataa attttatctt taggtattcc
    22501 caatattttt gtatgtacta acaaattttc ctctgctgta aggtttccat acaatgcagg
    22561 ggattctatt aaagaaccta tttttgcaag agattctcgt ttccaaggct ttctatcaaa
    22621 aataatacga ccatttgttg ggcgaagaag accaacaagc attttaagtg ttgtggactt
    22681 tcctgctcca tttggtccta aaagtccata tatactattt cgttcaattt gtagagaaac
    22741 attgttaact gccatttgtt ctccatatct ttttgataag ttttttgttt caagaattaa
    22801 atctttcata ttaatttctc ccttcttgta ataccttaaa tctgttacta agagaatact
    22861 aacatgaaaa aataacaatt ctataacacc tactaatttt aataaaattt aaaataaaaa
    22921 atagagttac caattttatt ttggcaactc tatttaagtt tatgttttac agttatatta
    22981 gatttaaact tagtttcatt atcacactaa cttcaaattt actctataat ctttaaatca
    23041 aacactaatt tattaatcaa acactaatct attaatttat atttctttag acatctaata
    23101 tagtttgcta ctaaataata attatttata tcatatttat ctaatccttt aaaagaagtt
    23161 atttcatcat taagctcatc actaccttct acaaaaacac aacctgtttc aaattccata
    23221 cctacaataa tcttacagtc actataaatt tcctcttgag tatcgtacaa tttttttgca
    23281 tttccttcat ttggaattgt aaatacaata ggttttaatt ctttatcact ggaaatcttt
    23341 tgtgaaaaag gcttgtttaa cttaatatta ttgataacta catgtttaaa ttcctctgaa
    23401 gtttcttcta ctattacata ttttcgcttt aaaaactcta ttatttctaa aacagactta
    23461 gaatttactt ttaaaatact tttatatttt tcatataaag atttccactg ttcaattttt
    23521 tcctgtggta tttcttggat atgtgtactt attttttctt gagatatttt tttcaattaa
    23581 actctccttt caatacttaa atatccttat gaatctcaca tgactaaagt tacaaggttt
    23641 ctacttaata gaattttgca tacttaaata tgtcttatta attctctaca ggatatctcc
    23701 gtagttccta cgattcttac ataagttact tattttaaat tgttattagt tatattctaa
    23761 aaattattta ttttatcact aatataatac tcttaaaaac tttacaaatc aatttaaatt
    23821 tgctaccttt tatttataac aacttatagt acatttctcc tatgcttttc aatatattac
    23881 ttttaaaagg aggttttcat tatgatttta aatagagctg agcttttccg aggatattct
    23941 ttaaatagat ttaacgagat atgcaatata ttatcagtaa ataaaattaa atataagtat
    24001 aaagtgaaaa ataatgctta ctctaaaaaa ccttttttta atgaaataac acttggcaat
    24061 ttaggccaaa aggaagattt ctcttatgag tacttaattt atgttcataa agacgattat
    24121 gaacaggcag agtctctaat aaattctaag ttgcgataga ttatctataa aacatgacat
    24181 tctgttgtca tgttttatat gttattatac tttcaaatat gctactatat tttcaaaatt
    24241 gtgtgcttta aaactagctt tttttagtaa tttaaagctt aaaactaagg agactcgtta
    24301 tatgaaaata aatataaaat caaagttaag tgaatttatt aaacagaaca atctttttga
    24361 tgacagcaga ataatatcct atcttcctaa tataacaata gaaacagaca aaataaaaac
    24421 tataatgatt aatgaggttg tcccaactaa caccaatgac gatttttata gtgtagataa
    24481 agatgctgat tatttaaaaa ctactattcc tttatttgac agtgcaggaa taaaagtatc
    24541 taacataaat gatatcctag atatgggtat ctatataact aatgcagtaa aacttcctaa
    24601 atcagaatat acaataactc gtgatacaat taaattacat atgcctattt tagaagaaga
    24661 aattaaatta tttaaaaacc tagaggtagt aatgttaatg ggagatgttg caaaaaaatc
    24721 ttttaatatg attactaaaa agcatataaa aaagaatgtc atcccttcca tttcaacata
    24781 taaacttcgt aataatgaac tttattatga taatttaaga gtatttccat catatattat
    24841 gactggtgga aacatactga ttgaaaaatc aaaatttgaa atggcatcag aagatataaa
    24901 aaaaatgttc gagattataa acggttgata ttacaaaata ataaaaacta atataaaata
    24961 aaaagtgcct caaaaacttc tatttatgag gcacttttat tttatattag tgtaactata
    25021 tactattaac ataatttatt atttatattg ttctttttaa tctctattcc atttagtcta
    25081 ggtaacatta ctctttacaa gattacttta tatttctttt atatctctaa cgtttttatt
    25141 taataaaaca attattaata caagtcaaat atatctattt atctaaaaat atatctttta
    25201 cagtaatatc tggatggtga tacttcttcc tattcgttaa aaaagatttt tttacattaa
    25261 tagcctcttt tggacaccag tgaaaacaag ctaggcatct ttcacactca ttaccccact
    25321 ttggcttttt atcaactata cttatattct ctacaggaca aaccctttta catgtcccac
    25381 atccattaca tttattttct gcaaaaaatt tagtagttgc aatttgcata ttcttaccta
    25441 ctaaacgaag tacaacacta tctatatgtg actttaaatt gttttttcct tctatatttc
    25501 caatctccaa gttattgata cattttgcaa tctcagctac cctattagtc tgatttttca
    25561 atctttcttc ttcaacatct ggaggtgtta tcaaagcatt tccaggcata ttgattaaaa
    25621 aaccagcaga taattttttc cccctttttt taagtaactt atctatagta aataaactat
    25681 gtccagctac cccattacaa gtggcaatgg catatatatg agtattaata ttaaaattta
    25741 atttctctac aaaagacttt actatatcag gaacatcagc aaaataaaca ggaaaaacaa
    25801 ttaccaatat atcttcattt atgtctatat tattttcttt tctatgtata gctaagggta
    25861 taacttcttt acttccattt aacgtttctt ttaaaatatt tgaaactact agcgaatttc
    25921 ctgttccaga aaaataataa atcttactat tcattcttat ccctctttct atatttctac
    25981 atcatcaata gttaaaaagt ctcttaaaat aggatttata tttactttcc aattctatcc
    26041 aaaatatcag taaacaaagg caattcttca tcagagatag atttaaatgt ctctgttgca
    26101 tatatacttg caatttcatt taatggttct tctaattctc tacctaaatc tgttaggaat
    26161 atttgaaact tacgtctatc tttaacatct actttacgga caattaatcc cttatttttc
    26221 ataacatcta aaattcttgt tgtattagat ttatccttac atgatttttc acataaatct
    26281 atctgagaaa tcccatcttg ctcccataga aaccttaata acgcataatg gtcagttgtt
    26341 atatcatatc cattttgttt aaacatcttt tttaaatcat cttttacttt taacgaagta
    26401 cttactaact tagttgtagc ttcatatagt ttctcaaaat ctttcatttt acctccattt
    26461 aaataatccc ttgctcctcc catctatcaa ttattccctt atattgctca atatctttaa
    26521 aagccattgg ctttaaaaca gcatttatag caataaaatt atagatttga gaaagcacag
    26581 gtggattttt cttttcctct atgcaataaa taaatttttt agataccttt tgtatctttg
    26641 attctacttt ttcagttata ggattttgat taaatccagt ttttatagat aaactaccac
    26701 aaacataacc tcccgaatct aagaccatgg attttataaa agaaattgct tttgaaccac
    26761 gtgcattttc atatgtagta actgctatac atggcttacg atacaataat ctttcaagca
    26821 tcatatgtgc tctatctgta aacaccttga aaagtccagt tacactactt ccataagttg
    26881 ggctaccaaa tataacacca tcactcttag taattatatc atgaatatac tctactttat
    26941 cattttgatt gatacacttg cccattttat aacaatttaa acaaccaata caatatgatg
    27001 gatttacctc acttaaatca aaataattaa tttttacatc tggtattaat ctttctatat
    27061 ctttatacaa gtaagttaaa acttttgatg tagctccatt ttttctaggg cttccattta
    27121 taactgtaat tatcatttct atatctcctt tttagttgtt atatcaacaa taagtatatc
    27181 aactataatt ttagctgtca atataaacca tttatttaaa gctatactta cacagcttaa
    27241 ataaaagttt tatttaacta catgtgctta tttaatcaac tataaatgaa ttatttttaa
    27301 taattgacaa actaaattaa tgataatata ctttatgtat tacaaatgta agtagaagga
    27361 ggtatagtat gaaaaagtta ccacatattt cagaagcaga atatcaagtt atgaaaatta
    27421 tttggaaata tgctcctatc agcactactg aagtaattga aaagttggta gaaacaagta
    27481 catggagtcc taaaacaatt caaacaatgt tattgagatt agttaaaaaa ggagctttaa
    27541 catatgaaaa aaatagcaga gtattcgttt atactccact tgttaaagaa gaagaatacg
    27601 tagctacaga aagtagttct tttttaaatc gtttttataa tggtactctt aattctatgg
    27661 tactaaactt tttagaaaat gataaactat ctgaagatga tattgaggaa ctgagagaaa
    27721 tcttaaataa aaggacgact aagggggata aataaatatg tatgccccat tctttatacg
    27781 atttttactc agtacattaa ccttatcaat attaattgct gtaattttac ttgccaaaaa
    27841 agtatttaaa aaacatattt ctgcaaagta ccaatacaat attggattta tattgcttat
    27901 aatgttaata attccatttg tccctcttaa atatattaat cttggaaatg tatttaacta
    27961 tttatatact tttaatcaac ttggaagcag tactaatagg acttctatga taaaaaatag
    28021 tccaaattct ataattaata actcaaatgc attaaacgat ttttcagtat ccctaaacca
    28081 agttaatttt gatagtttaa atattttatt actaatatca tggataattg gaattttaat
    28141 catgatagct attactattc attgcaacat taaaataaaa aatatgaaaa aatcaataca
    28201 aattttaaga gatgaggaga tagtatttat atttgaaaag tgtaaaaaag atattggtat
    28261 aaataaggat tttatactat gcaaatcacc tattataaat acacctatta catttggata
    28321 ctttaaacca cacattatat taccagataa gtctatatct aaattatctt taaaagatat
    28381 aaaatatatc ttacttcatg aattacaaca ctttaaaaat aaagatatac tgataaatta
    28441 tataatgtgc ttccttcaaa tattatattg gtttaatcct cttgtatggt atgcatttaa
    28501 agaaatgcgt atagaccgtg aaattgcatg tgatatttca gttttaaaaa agcttgataa
    28561 agatagtcat atagaatatg gacaaactct aataaacttt gtggataata tttcaagacc
    28621 atcaaatctt acacttacat cagatattgg tgggtcaaaa aaacaaataa aaaagcgact
    28681 attgaagata atggattttc ataatgaatc aaatacgtta aaattcaaaa gcattttaat
    28741 ttttacattg atagctttaa ttgtatttgg aaatattccc aaactatcta ttatggcaga
    28801 tatcaatgaa aaatatagac ctacaaatga acaaactgtg tacgaggact taagtcagta
    28861 ttttaaaggt tttgatggaa gttttgttat gtatgattta aaatccaagc aatatcatat
    28921 ttacaataaa gaaaaaagta aaacaagaat ttctccaaat tctacttata agatttatag
    28981 tgccttattg ggattggaaa atgggataat aacaagaaat aattcacata tcccttggga
    29041 tggaaagcat cattctaata gttcttggaa tgaagaccaa gacttatttt ctgctatgga
    29101 aaactccgta aactggtact ttcagactct agataaaaaa acagggctaa aaaacataca
    29161 aacttatctt aggcaaattg gatatggcaa ttatgatgtt tcaggtggta tctcaaactt
    29221 ttggctagaa tcttcattaa aaatatcacc tattgaacag gtagaattac ttgaaagttt
    29281 ttatacaaat aagtttaaat ttgaagataa aaatatagaa acagtaaaag attcattgtt
    29341 aatatcaaaa aaggatgggg tatcactttt tggtaaaaca ggcactgcca atataaatgg
    29401 aaaagatgta aatggttggt ttataggtta tgtggaaaaa aatgaaaata cttatttctt
    29461 tgctacaaat attcaaagca aagattattc taatggtagt tcagcatcta agataacatt
    29521 atccatatta aatgacaaaa aaatctataa tccttaatat atttactgta cattctaaaa
    29581 aattcacata attttcatat taattatcaa ttaaattcat atacaaggct tattatataa
    29641 acatagaaga tagtaaaact agcttaaaaa cataatataa taccctatac aaatagtggc
    29701 aactctccct cacatcctca attgttgcca ctatttaatt ttttgtatga agttaaatat
    29761 attttcatca tttcaacaat ttgggtgcaa tacaatctct attttgaaac aacctcttta
    29821 attgttttag agtttatgaa ttatttctca tactttaaaa agattgaatt actcttgata
    29881 atcttctttt gtagtcatta catatcacct actactcaac acatattctt tgacctattt
    29941 ttacatcctt gtttatatcc aaaatattta ccttcttacc atctggaaac aataccattg
    30001 ttaaagtaga ctttcctaat tcatgtattc tctttaaatc aacattaaca agaacatctc
    30061 cctgtttaac catatctcct tgtttaacag caatatcaaa gccatctcca tttaatgcaa
    30121 ctgtatcaat tccaacatga attaatactt ctccattagt tcctttaatc ccaaatgcat
    30181 gtccacttga aaccactgtt tctaatttac ctgaaatagg agaaacaatt tttccatcta
    30241 taatatcaat agcaaaacca tcacctaaca tttttttaga aaatgtttca tctggtacat
    30301 tttctaatgc aacaattttc cccttagctg gagacacaaa gttttcatta ttttcattaa
    30361 acactaattc ttcgtcttca tcttcattta tagcttcttt aggcactcca aataaacatg
    30421 ttacaataaa gcagaatgca accacaaata ttgatgctaa taagtatgtg attaaattat
    30481 atccatcata gatatacatt aaataagaag gtaaaactgc taatccatat gaattagatt
    30541 gaatatgtaa aattgataat attgctgctc ctaaacctga agatgcacac ataataatca
    30601 aaggctttaa attatatcga agctgaatcc caaataaaac aggctcactt ataccaaaaa
    30661 gttgagatag cattgctcca actgcagttt gtttaacttg attattttta gattttttca
    30721 taaatgctag acatgttcca atattcgcaa atccatacat tgcacaaaga gtaataaggg
    30781 cattaaatcc tgtatttgct aatagtgatg tttcaatcat tacataagta tgatgtaagc
    30841 cagtaataac agctaatgga tatgtaaatc caataataaa tcctccaatt cctaaaggca
    30901 aatcaatcaa taagctgatg atatcaacca tttttaattc tattgtatgc attatagggc
    30961 ctacacctaa caaaataact aacattgtta ttagcataac aaaaaatggt gtgaaaataa
    31021 ggtctaatac attaggcata tgtttacgga accatttttc aagattagct ccaataaatg
    31081 ctgtaacaat agctgttaaa acacttcctt gacaacctac aataggaata aatccaaatg
    31141 ccataattgc ttcaatttca tttgatgggt cagcaacaga atatgcatta ggaagtattg
    31201 gggaaactaa cataagtcca ataacaagtc caataacagg tgttcctcca aatactttaa
    31261 atgctgacca tacaataatt gctggtaaaa aagcaaatgc tgtttctgta agaaccgaaa
    31321 caagtgtctg aatatacaat ggaatatttg cagaagacat tccaaataat ccaagtacat
    31381 tatcattaaa caaacaaccc tttaatccca agaataatcc tgttgctgca ataactggaa
    31441 taataggaac aaatatatct gctagagttc tcataacttt cttaaatcca gtttcattat
    31501 ttttaactaa ttcatcttgt tctttttttg ataaagtttt taatcccatt ttttcaactt
    31561 ctgcataaac tttattaact attcctgttc ctaaaataat ttgatactgt ccagaagtaa
    31621 agaaaacccc tttaacctca tcaacttttt caactttctt atcattaata atttctctat
    31681 ctttaaccat cactcttaat cttgtggcac aatgtgtaat agaaataata ttatcttcac
    31741 caataatttt aataagctca tttgctattt tattatattt attcattttt ctacctcact
    31801 ttcaataata ctgcatgaaa tggtttcaaa cgcactgttt tatttaatag atttatatca
    31861 tctaaatttt ggaatacaat atcatatttt tcttctactc ttatttctac aggtttatca
    31921 gaaaaattat aataacaatc aataactacc aaatctaatt gccttctata cgcaataata
    31981 tcatcacttt ctatatcaat aggttctatt gaaccataaa tcaaacaatc agaatactca
    32041 ctatcttgtc tcaactttat catcttttta taaaattcta aaatactctc tgaattgtta
    32101 atctgactct gtgcattaat atctttataa ttttcaataa cctttaacca tggtgttgta
    32161 tctgaaaaac ctgcattttc tttatctgcc catggaaatg gcgttcttgc attatctcgg
    32221 cttcttaaat tcacaaattc taacgcttct tcttctgagt aaccttcatc tattgaacga
    32281 tagtattggt caatgctaga taaatcatta aaatcattta tggtatctct tttaaaattt
    32341 gtcattccta attcctgtcc ctgataaata aatggggttc ctcttaaaaa gaaatacatt
    32401 gctcccaaca tttttactgc atcatcattc ctttcgtatt gcaaaagata ctttgttgta
    32461 gctcttggtt ggtcatgatt ttcaataaaa ttagctgccc atccatgttc ctgaacatac
    32521 atttgactat ctaaaatgca ttttcttaaa tctggaattg tccatgaaat tcttttaaac
    32581 cactcactac cagaagcaac atctaagtca gcatgtttaa agtcaaagac cattgaaaag
    32641 aaaccatctt ctccaataaa atcacctaac tggtcatagc taatacctgc tgcttcagca
    32701 acagtcatac aatcatattt tttaaacgta ttttctttta attcataaag cattttttct
    32761 attccaggct gatttctaac tgtttttgta catttaacaa gaccatcaac accatcactt
    32821 tctaaacttt gaaaacttaa attctttttg atgaatgtaa tagcatcaac tctaaatcct
    32881 gccactccca tttgagacca ggtattaatc atttggtaaa gttcacttct taatattgga
    32941 ttttcccaat taaggtcagg ttgttttttt ccaaaagaat gaaaataata ttcgtctcta
    33001 ttctctacct tttcccaaac acttccacca aaaacagacc tccagttatt tggcaaatcg
    33061 tttccttttt taaaaatata ataatcatga taaggacttt ctggattttt taatgcttct
    33121 tgaaaccaag gatgttcatc acttgtatga ttaagaacta aatctaatat aattttgatt
    33181 cctctatctt tacttttttt aattaactct tttaaatctt ccatacttcc aaattcactt
    33241 gcaatatcca aataatcact aatatcatac ccattatcat ccataggtga tttaaaaatt
    33301 ggacataacc acaatgatgt aatacctaaa tcttttaaat aatctaaacg agaaataatt
    33361 ccctgtaaat ctccaattcc atcattatta ctatcttgaa agcttcttgg ataaatttga
    33421 taaacgattt ctttttgcca ccatttcttt tccataaatt actccttttt atatgatatt
    33481 tcttcttttt aattctttat aaattccatt atcgaatatg tcctcacata tagatgtcgc
    33541 tatatcttta agttgttgat gactattttt cattgcaatt gtaacgtctg atgcttgaaa
    33601 catgacaatg tcattttgac catcaccaaa acatatggtt tctttttgag ttactcctaa
    33661 tctttcttgt aatctcttaa ttgctttgcc cttatgaaaa tctttttgaa taatttcata
    33721 atactgacta gaaatatctc tctgtgctaa ttccatttta tcttgtagta tatctttcac
    33781 ttcgtcaaat accttttcat ttgaccataa acaaatttta tgtatatctt gtgacttata
    33841 ctcttcaatg ttattttcat atgttatttt ttcttggata tgttgtttat ttatacaaga
    33901 attagttcct ttcaactgat tcattgtttc aaatatttct ttggcttttt gattcatgaa
    33961 aactttttcc tgactctcaa tcgaaaaagc aacttctcta ttttttaata aacaaacaac
    34021 ttcctttatc aatctttgat taaaagattg attataaagt aattcaccat gatattgaat
    34081 gtaattacct cctcctgcaa tatatccatc aactcctaat gataaaacat catcttgtat
    34141 tgtacccata cttcttccag tacaaataac aacactgcaa tgattctttt gacataaacg
    34201 tatagcatgc tttgcagatt ctgggattcc atatacctca tctctaagcg taccatctat
    34261 atcaagtatt aataatttgt acaatttcat cttcctttct tactatcaaa atcataatag
    34321 cattcaaaaa aaaagatatc aatattttga tatcttttaa aaaatatgtt catttttagc
    34381 acaaattttc attttcgggg aatatgttca ctttctaaat acatcttgta tttaatataa
    34441 agcagttcaa ttaaaataaa ataaggtgtt gttacatcgt agtccagttg tttatctaca
    34501 gaaatagata tagattgaat atacaggttt tcatcacata atcttgctaa aggattactt
    34561 atcatttttg taatagaaac catataaatt cctttcattc tcatttcttt agcaaaatca
    34621 ataattgtag atgcatctcc attcaatgaa ataaagaaaa tcatatcatc tgtttttata
    34681 aaatgattaa gtgcctgaat catatcataa ccataaacat catatataat tttccctgtt
    34741 ggtaagaaaa tccttttcat ctctttagca actctttcct gagaataacc ttctccaaag
    34801 acgattatac gatttgcctt tttcattctt tcaaacataa tcgaacaatc cttcttctct
    34861 atatcattta tcaccttatt atagcaatcc attaattggt gtttactttg atttttatat
    34921 tctacttctg tttcatttaa gcgtataata actcttaatt cactatatcc aggtaattgt
    34981 agtttttgag caaaacgaga aagtgcactt gtagatatat ggtatttgtc tgcaaattca
    35041 tctatagaaa gtttaataca atcttcttta tgattaagaa tacactcaca gatatactta
    35101 tcatttgctg taaatgaatt ataatattta ttgaaaagtc cttcaatttt cattcttttc
    35161 tccttctata ctctatgtaa ttcatatgaa gcaaatctaa tagagcataa aatgctccta
    35221 cgacttcata agatatagct tgtaatgtat caagtttttg agtttcaaca tacagattat
    35281 actcacaaag tgttgcaatt gtattattat caagcctagt taaagaaatc gtatgtatat
    35341 gattgttgat aaattttgca atttcaattc ctgcttttgt ttctccagac aaagaaatga
    35401 taataaataa atcacttttt tgaaatgatt ctctcataaa ttcaatttca cctatatcaa
    35461 ataagtcaat aacacattta cctactgaaa gaaatattct ctttatttct tgagctaaac
    35521 ttttctgttc attacctgtc ccataaatat aaattgtttc tgcatcatat attattttac
    35581 aaatatcttg aaatgatatt ttttgtaatt cattcattaa tagtcgataa ttctcgcaaa
    35641 catcatgaaa attattatca ttattaacaa attctagttg attattttta agtaaatact
    35701 ttaaatcaga gaatgaatct aatccaattt ttctacaaag ccttaataaa gtagctctag
    35761 aaacattaca ttcatttgca attttttcac acgaaaaatt acggtattct ctttgacaaa
    35821 tcaatatttg tttaataata attttatcat tttcatttaa attatcataa ttttggttaa
    35881 ctaacgattc taatctcatt ttttccactc cctaaatatt attttaatat acattagttg
    35941 ctttttctag ggaagagttt taaataataa ggataaaata atcatttgat tttattttta
    36001 ttaaaataaa aataacctgc taaaagcagg caccaaaaag tacttatcct ttcgaacatg
    36061 tactttagat attgctttct tcggaagctt catctcttca tactctaaaa tagagttttt
    36121 tctaactcaa aagtattttc catggtagaa attactttaa ttcatctgcc atttattttt
    36181 tatcttattt aataattact actacttgaa tatttacttc tttaagtaaa tttcatagtt
    36241 aacaaagcta aaattattat tatagatgtt aaaatacaaa attgtaagta ttgttataaa
    36301 tatgcttaca gctacaaata tacatgatat attaaaaaga agctgtaaat tattatcata
    36361 catacaagca actacgcctc tattaatcca tgttaatttt tattatttaa tacaaggagt
    36421 tccatgagac atacatatcc attcatattt taaattatcc aattctctta tagatttcat
    36481 catcttttta taatcccaat tccatggatt aggataagga actatatttc cattcttatt
    36541 tttgactaaa tcacctgcaa ataatacatc ttcaaaaatc atgcagacat gccctggagt
    36601 atgaccagga gtatggataa tttttatatt cccaatctgc atattctctc cataaggttc
    36661 aacatcctta atgagccttc tggaaattgc ctttccaata aacttcttaa acccaggtct
    36721 atccttctct cctataataa aaggtatatc ctctacatga gcccatactt ttgctcctgt
    36781 tttttctttc aataatttaa tattagatat atggtcaaca tcatgatgag ttaataaaat
    36841 atgcttaata tctgtaagtt taatgccaag tgtttttaat tctttaatta tatttttccc
    36901 cataaaagga agacctgtgt ctataagaat aatttcttta tcaaacacaa taaaacaatt
    36961 tgagccacga gtactatcta acattaatac attttctttt atcttcataa ataaattccc
    37021 cttatttggt attttaccta tataattaat taataaagtt ataaatagtt ataatacttg
    37081 atattaattt tattttttat taaagtatac tataggtata caaatattat atttacataa
    37141 atataccatc agtatacttt tttatcaata gaaaggaata atttatatgt cagaattatc
    37201 aaaaagagat aaagaaaaaa tacaaagaga aaatgagatt attgataagg cagaaaaatt
    37261 attttgtctt aatggatttg ataatacaac tatgaatgaa ttagcaaaag aagttgaata
    37321 tactaaacgc accatataca agtacttttc atgtaaagaa gatttattct ttgctgttgt
    37381 cttaagagga tataaaagac tttgggataa tgttaaaata gaaagtgcaa aaggcaaaac
    37441 agggtttgaa aaaataaaac tttcatactt tgcttttcat aaattttact ctgtagaacc
    37501 ttctctatta tgccttatgg gaatgattgg tattgtaaac tctgaaaaat cggatactga
    37561 tatgctgttt aaagaaaagt ttttttcttt taataaattt atgtttgatg aaatacaagg
    37621 aatgtttgaa attggaaaaa atgatagaag tattaggcat gatattgaaa tacctactct
    37681 tatgtattca tcaatattta cacttacagg attttttaat ttactatctg taacaggaaa
    37741 atcatacctg aacaacttta atatagatga ggaaaaattt attgaaacaa ctcttgctct
    37801 tttaatagat tcaattaaag cataaaccta agtttataaa ttaaagttta tatacttaaa
    37861 tgataatttt ttctatctct aatatgaaaa atattaaaca taaaactaat aacttagtta
    37921 atggaataac taaattactg aaaggattaa aagtaaatgg ctaaaatact tcttttagaa
    37981 gataatgata caattacatt tggaataaaa acttcccttg aaaagaaaaa tcacactgta
    38041 tgttgttata atacgattgc tgaagctaaa aaaaattttg ataaaactat tgatttaatt
    38101 ctgcttgatt taaatctacc tgatggtatt ggatacgagt tttgccaata tgttaaaagt
    38161 atagaagata ctccagtgat atttttgaca gtccgtgatg atgaaaaaga cattatacgt
    38221 ggattagata tgggtgcaga tgactatgtg gttaaaccgt ttttgctctc agtacttcaa
    38281 tccagaatta atgcagttct tagaagaact cattcaaata ctaacaatac tattacatgt
    38341 gaaaatataa gcttgaataa ggttgaagtc aaagcatatt tagataatgt tgaagtacct
    38401 ttgacagcag gagaatataa acttcttcta agtcttatgg aaaataaaaa tagagctatg
    38461 actcgtacaa agttactaga aatgctctgg gatgtagatg gtaatttcgt aaatgataac
    38521 actcttactg ttacaataaa acgtcttaga gaaaaattaa aaaatccacc ttgtataaaa
    38581 acattaagag gcataggata tagaatggag gacaactagt gaaaactcca agaaatatca
    38641 acagtacagt tgtttttata atattctgct taattgtaac ttttttattg agtgtaattc
    38701 taacaactat aacttctttt aatgaataca atgtactgtc taaggctctt ggtgcatcat
    38761 atgagtacaa tcctaagtct acagaaagta tactaaaatc actgaaaaat tctagtgata
    38821 aggattttaa aacaggaaat gaaattttat caaaacatgg atattctaaa agtacatttt
    38881 ggagtaaaaa ctttttttat tttctagtga tatcgtttgg tataatttca acaatggcta
    38941 cagttgtata tataatcaaa catagaacaa ataagcaaaa actgagtaga ataaacaatc
    39001 ttactcaata cttagaagaa gtgaatttag gaaaagagtc tgtactttta cgatgtgaag
    39061 atgaattttc tcaccttgaa gatgagatat ataaaacagt tggggaactt cgttgttcaa
    39121 aagaatttgc tttaaaagaa agacaaactc tttcagataa ccttgcagat attgctcatc
    39181 aacttaagac tccaattacc tctatgtcac ttatggcaca attactttca gaaaattgtc
    39241 cagatgaaga aatagattat attaacagac ttacaaatca aatctcaaga cttgagagac
    39301 tagtttcatc tttacttaca ttatcaaaat tggatgcaag tactttaata tttgaaagca
    39361 aacttataga tgtacattct cttcttactt atgcaattga gcctatagaa ggttctttaa
    39421 gagaaaaaca tcagacattt actatcgagg cttctccttc ggtagaattt gaaatcgata
    39481 ttaattggac tttagaagct cttttaaata ttcttaaaaa ttgtagtgag catatagaag
    39541 atggtggata tatagttgct tattatagtc aaaatcctct atatgttgag attatcatag
    39601 aagataatgg taaggggttt gctcaggaag aacttccata tatttttaat agattttatc
    39661 gtggtaaaaa tgctagtaag gacagtattg gtatagggct tgctctatca aagtctatta
    39721 tagaacgtca aaatggtact ctacatgcag aaaattgcca taatggtggt gcaagattca
    39781 taattaaatt ttacacataa aatgctggat tataggtaaa atacctataa tccagctata
    39841 tctatattct atgttatcta ttgttttagt aatatacttc tatttacatt taaggtttta
    39901 ttaaacaaac aacttaacta tccctatact tgtaagtagt cttaaatcga aacctttaaa
    39961 taagttcaaa tagattttgt ttatattgtt tcatttttta tagattctac tatattttca
    40021 ttcttcaact gcttgtatcc tgtcatatat gatgctatta caataaaaag tatcaatata
    40081 gtaaaaccta ttattgtaga tataggtgca aatggtaaat actctataaa atatatttca
    40141 tttatacgta gaaaagcata cactattcct atttgcacag gaatactaag tagtaatggt
    40201 gtaattccta agaataaacc ttccaaaata agcatctttt ttatgccttc tggagataat
    40261 ccaacagaac gcaacatagc aaactcttgt cgtctacttc taatatttcc tgacactgta
    40321 gcaagtacat ttgataatcc aatcatagcc aataaacctg taataaagcc tactattaaa
    40381 ttcatggtct ttattccact agccttttgt ttttcacttt ctaaaacatc ctcaatacta
    40441 taatctcctg aaccatagtc tttattataa atggactcta atttttctct tactctagga
    40501 atgtcctcac gattatttac tttaaatact cctatcagcc tatttgaacg taacctactt
    40561 acttcatcat aatttttaga tatttcctgc accatagaca ttggaactat ttgcattaat
    40621 gtatactttg aactagtatc tactatttta ggaagtgtgc ttgtaatagc tcccactttc
    40681 atgttgtaag tataatcccc tttatcttcg tcatatactt tttcatttaa tttaatgtta
    40741 tctcctattg aaagatttaa ataatctata tatactttat ttctacgtgt actacgatta
    40801 atatcttcct ccttattata tacaattgag gttggatttt ttgtatcata aaataacttt
    40861 gggtctacag aaatttgcct acaatattct gtaaaactct tatcatcaag cccaattata
    40921 tttgaaatta tacgaaactt gccatcacgt tctataggag agtacttttt agatgaagct
    40981 atatttttaa atcctccatt tttcgcaagt tcttttgaag catctttttc agtcaaccaa
    41041 gtggaagcag acattttttg ataatatagt gaacttttta caccttctgt attatctaat
    41101 ttttcaatga aagcagactc tacaggttca ctattttcca aataaactgc tatatctctt
    41161 tggtccttat aattatcact tccataaaca gcttttgctc cttcttgatt tgcaattata
    41221 tgcaaaaaac atgtaagtaa taaaaaagat aatgttaaag atattgttgc agtacgataa
    41281 gattttttac gagcacgtag tgcgtttgat gcaagttctc cttctattcc aaatatcttt
    41341 cctaatgaat atttatgcga ctttttgatt ttaacactat ctccttgtcg tattgcttct
    41401 ataggagata tctttgatac ttttcgtgct ggtataagtg cagaaatcca gactgttact
    41461 attgtaagta aaactgctgg cagaaaagca ggaataccat aagtaaatac cacttctgga
    41521 acatcagtat gataattaac tgaattaaca taaactataa gcctattaca caataaccat
    41581 cctaaaaata aacctattgg taatggtata attgtcagta aaaagccttc aaacactaca
    41641 gaccttttaa tttgttttgg tgatgcacca acactagata atatccctaa ttgagtaagt
    41701 ctagcatttg cagacagtga aaacgcgttg taaatcacca ttacaaacac ggctactgta
    41761 aaaattgcta ttgtaagata cataatagga gttgaaaatt ttgatatact actcatagat
    41821 gctttctgtt ctggtgaaaa gactaacatc tttaaaagat aattactatt gtatttaaga
    41881 ttgtattttc catattcatt agtcttccat cccaatgatt tagcaagttt tggaagttcc
    41941 ttatacgtgt ctcttatatt tttaaatcgc aagtacacag ttatactatc attaggtttt
    42001 atacttgttt tatcaagata tccaagagct gtatatgcag gtattactga tgaagtagaa
    42061 acatctacct ttcctactat tgtcaatgta gcctctgaaa tttttttaaa actttcccct
    42121 ttcgattttg gggctatggg ttcaagaggc tttccattta ggattctatc tcctattggg
    42181 agagtaattt tatcaccaat ttttacactt ggattattct caaaatattg ttttgataat
    42241 gcaatttcat tagcattatt tggtcgacga ccctctatta tagtatcctt ttctggcata
    42301 gaattccaat actctgatgt agcatttcta taaattatat aatctctttt ctcatcatta
    42361 attttgccta cataccattg tccttttacc attacatcat caactgaaga aaaattttca
    42421 attaatggta agtcacttcc ataagtatag tcaaacaatt caccatgcca gttaccttct
    42481 ttctcaattg aaagttttat aaaatctgtc cacatggtat acaacagtcc acacaaagaa
    42541 gacatcatgg ttgtagtaag taatatagct aacattatag ctatactact acgtttattg
    42601 cgtcgtacaa agtctaaggt gtattcttga agaatattca tttcttcacc accttttcag
    42661 agacaatcac tccatcttcc atagttacaa tacgatttgc ctctagtgct attttctcat
    42721 catgagtaat aagaagtatt gtctgattaa atcgtttatt tgataagttt aacaagtcta
    42781 caatttcttc tgaatttttt ctatcaagat taccagttgg ctcatctgct aataaaatag
    42841 ctggtctata gattaaactt ctagctatag ataccctttg ttgttgtcca cctgaaagtt
    42901 gacttggaag atggtttaat ctatcagata atcccaatat agatacaatc tcagagaatc
    42961 tatcctcatc cacctttctt ttatcaagta acatgggtaa taatatattt tttctaacat
    43021 caagggtagg tataagatta taaaattgat aaataagacc tacttttcta cgtctaaaaa
    43081 ctgcaagagc ttcttccttt aaacttgata tttctgtatc ttcaacataa atctttccag
    43141 atgtaggtct atctactccc ccaagcagat gtaacaatgt agatttgcca gaaccagatg
    43201 ctcctacaat ggatacaaaa tcaccttttt gaactgataa atttacatta ttaagggctg
    43261 tcactctggt ttgatttgaa ccatatattt ttgtgagatt ttcacatttt aatatttcca
    43321 tatacaatca tctcctttat ggttaaattt tagcaaccta aaatgacatt atagtgacat
    43381 gaatacaaat ataaattact attaaaaata ttgtttagtt tttgcacatt atttaaaatt
    43441 ataatataaa acaaaaacta tgcaatatat tttatttata cttttaatat attacatagt
    43501 ctttaaaagg tattaacttt aaatctatag gtattcaatt taataatcaa tagcaagtat
    43561 tttatgtact aaaggagttt tgctctatat agactcaatt tagtaaaatt atttaataaa
    43621 tattacagac tataggtaaa taatatttac aaataaaatt aagtttattc ccttacacta
    43681 taatatttct taattatatt aattgcaatt tcctttacct ttggccctgt aatttcttta
    43741 ttggaatcat ctgatttaat agcaaaataa taggtgtcac cttcacgctc tagcatacca
    43801 acaaaccatg catccacacg tttatttttt tcatcaaatc ctgttccagt ttttccatat
    43861 acatttatgt ttttatcatt tacatctatt ttcataatat ctcttaaaat attaatatgc
    43921 tcttttttaa agtttgtatc tccctcaaat atttttgcca ttgtttgcac ttgttctttt
    43981 ggagatattt gtaatgaaga ttctaaccaa aatccattta aatgtccctt tccatttttt
    44041 aaatctcctt cccattcgct aatatcgcaa ttcccatact ttaaatcatc taaaatgttt
    44101 tggacagatt tagcatcaac tttatctatc aattttttgt agtaccaaac acaagactcc
    44161 ttaaatgcct cttctaaact aagattttta ttccaatttt tatttggata ctctgtacca
    44221 tcatatccca taactgattc ttttgagttt attacccctt tttctaaacc aattaaagtt
    44281 gaaactattt taaaagtaga acatggtgac cttcttgttt caattaattc tttgttatat
    44341 atattatatt ctttattttt agtattataa aaaatagctc ctccactaat accttcaaaa
    44401 caatcactat agtcaaccat attaattttt tcttttttgt ttttattatt gttttcaatt
    44461 ttggatttat ttaattcaga cttatttaat tcagatttta tataattctt atcagttttt
    44521 tgatttatat cgttatgttt tttccctaca taatacatta ccataactac accaaccaat
    44581 aagatagcta tccaaataaa attctttttt cttttcataa tttatatcat ctcccatatt
    44641 taaatttata acctactatt aaaatagtag acattatttg taatatactc caatctaggt
    44701 tctacttata agaaacaaga ctaaatgaat agctgtatct cttatatttt cttcacctag
    44761 tacaattatg ttgattaaac ctattgcgaa gataattaag taactattaa taacatcctg
    44821 tcctcctttt aatttgtgat ataataattg attccacatt taaaacttac tattgtaata
    44881 tttaaatatt acaatagtaa gttttaaatg tcaagttagt ttgggaaagt tattttaatt
    44941 gtataattgt tattacattt ataaaatatg gcatgtttaa aattattttg ctattgggag
    45001 gattaagata agcagtatta taaaagctat aaattataaa atttaatata taatttatag
    45061 cttttacttt tacaaatatt gtttttattg atttattaat gaattttatt gacaggttat
    45121 atttatataa gtaaacactt acataataag tatataatct acatatctat aagttgagtc
    45181 aattcaccag tatatgtcaa cttaagttta tgcatagctc gtgtacaagc aatatataac
    45241 aaactgcggt cataatcact ataatatgtt ttattattta ctgaaggtac aataacttca
    45301 tcaaactcta aaccttttga cattttaata gaagttatgg atactccttt tgtaaagcta
    45361 gaactttcag aagatattaa gtgaacacta tatttttctc ttaacgcatc atatacagct
    45421 ttagcgtcat tatctgtctt taatataata ccaagagtcg cattatcact ctttttaaat
    45481 tcttcaattt ctatttttat cttatttatt tcatcttgct cgttattaca ttttaataaa
    45541 gctggttctt caccatgtcg ttcaataggc tctaatgaag atatgtcttg aatctttttg
    45601 gcgaaattaa taatctcaaa agtagaacgg taactcttat tcaacataac tagttcccca
    45661 tcattatata gctgtttcat atcatctagt gtatgagtgt gattaggatt aaccaattgt
    45721 ccaaaatctc caagaatagt ctttttacac ttaaaaagta agtttattac agcatattga
    45781 ataggtgtgt aatcctgcat ttcatcaata acaacatgtc ttataatctt atcttcttgt
    45841 ataccttcat atgcagcatg aatatatata aatgggtata catctgacca ttcaagagtt
    45901 ttttttgttg ccatcacaaa catgtttgat atattcattt gtttaaagaa atccttatat
    45961 agtgtaagag tattttttat tgtcaacatt ccattcaaac ttttaagtat actttttacc
    46021 tttggtaaat cttcttccat aatattatct gattcaaatt tataatgaat gtcttcagct
    46081 accttctcta atctcttctt aactggatat ctgttatatg cctcaaatct actctgtatc
    46141 cagtcacttt tagctgtaaa acttccaaaa gtgtagtctt ttggaataaa aatcttattt
    46201 ggcatttgtt ttatataatc atcaataagt tttacaaaat caagtgtaga tttaaaacgc
    46261 actctttcag accatttagc atcatttatt tctaatgggt ctttctcact ttcaaaatta
    46321 ataactctat ctaattgtac ctctgctata ttttcaaaac ttaattcaca taatggctct
    46381 tctccaagct caggaaggac attggaaata tagtctccaa agactttatt aggtgatagg
    46441 ataattacat tatttgctga tattttatct ttaaatcgat aaagaagaaa agcaattctg
    46501 tgtaatgcta tagatgtttt cccagaacca gctacccctt gaataatcag tgtatcagct
    46561 ttatcatttc taataatttg attttgttcc ttttgtattg ttgaaatgat tgacttcatt
    46621 ttttcatctg aagtgtgact aagttctttt tgaagaatat catcttgtat attaatggaa
    46681 ctttctagtg catattctaa ttttccattt ttaattttaa attgtcgttt tcttgttatt
    46741 tctccatcca cccatcctat tggagcatca tatccagctt ttccgatttc ataatcataa
    46801 aacatactgg caattggaga acgccagtca agtattataa gctcatcttc ataatcaaat
    46861 gcaaatctac caatataata cttagattca tcatccatat ctttcagcct aaaatctatt
    46921 ctagcaaagt atggagaatc tttgatttta ttaatcttat ctcgaacttt taccataaaa
    46981 gccccatagt tatctatttg ttttagtcct tgttcatttt gaaacatttc atgtgggtct
    47041 atttcaccac gattttttac catatacatt ttagcgtcca tataatcctt atcaagcttg
    47101 tcaacaagat tttcagcttt cttaaaagca ttgtctagct tgttatttat ctctattaga
    47161 tgtgcaatct catctggaaa aaaagttaat aactctgcat catattcaca catattatgt
    47221 ttagaattag atgcaataat atcgcaattt actacttgtt tattttcaga catttaaatc
    47281 ctcctttgtt aattaagtac ttacactgaa tcattatact taattgtgtt ttggagtttt
    47341 taatcattct tgctatttaa cttaaatgtt agctttttag ctttaaagtt ccttttttaa
    47401 aaaactaaat ctttctatat ttattaccat aagtacactt acaagcaaaa tacattaaaa
    47461 gcagaaatgc tcttatatat aaaataattc aaacaaaaaa tatgtggtaa acttttcatt
    47521 taatctacaa ttaattttgt ctattatctc tttcttctac acaaggaaga ataaattttt
    47581 gagtaggata tccaaattcc acaagcaatt ctgcttctgc aaagcccaac ttctttatca
    47641 tatctctatg tcctgtatca gctttgtcac cctctcgaaa ggtagtaatt gaaatatctg
    47701 tatctatcaa gaaatcttca ataactttct tcaaaaacac ttgtgcaatg tctgaattac
    47761 gatactgtgg atgtattcca aaaaaatcaa tacttctagt ttctctatta aacaccatat
    47821 ttgcaatagc gacattatta agttttaata ttaaagctct ttcttctaaa atatactgtt
    47881 ctaattgata tatatattct tcctcttgca aatttggaaa tccatctatg actaatctca
    47941 ctaaattcat ccataatggt atgtcagaca tacttgcaag tttaatttct ttttccaact
    48001 ctagtacatt ctttgaaaat aggttatctt ttttcaaaac aaatcttagc tgcaaaggat
    48061 aaaattcttg ttctttccta tatttatttg gagatttttt atacatagct ttaaatatag
    48121 aagtgaatgc ctgttgactt tcatatccag ctatcaatgc aatttcaata attggtttct
    48181 ttgaaaatac caataatttt gctgcttctg tcaactttct ccgctttatg taatcatgca
    48241 ttgttagacc aacagaagtt gtgaaagttc tatgcaggtg atattttgag taatgaacag
    48301 cattagcaac tatatctaaa tccagctttt ctgataaatg ctcctctata tagtcaatag
    48361 cagttatgac attaattact ttgtaagaca ttgcagcttc cctcctttta tccatgcaac
    48421 attatatcaa ttattactta gttatattta atgaatcttg ctattttata aacaaatttg
    48481 ttgtttaagt tttcaattca taaatttaat atattacttt ttactttctg attaaagact
    48541 ctaatccttt aattttcaat aacttttcat ttagtaaatc taaaaagtta tttattattt
    48601 tcttcttatt agattccttt tatattcctt aattaaatga taaatattta tattatacga
    48661 ctaaaaacag ctttttacaa ttatttaata tgattacaaa cttaccctat cttaaagttt
    48721 aatggggtcc tgttatgaat ctttttattt taatttaagc tcttcatcag tttattttat
    48781 atattgatgt atttgatttc atacaaaaaa gagtgcttca tgaactaaaa agttcatttt
    48841 taaacactct cttttatgta aataaatttc tattctattt tgaaatatta taatttgact
    48901 tataatctgt taataccaaa aataataaat tgtacgtcta agaatagatt ctaattagtt
    48961 gctcagtagt aaaatctgtt tttaactaat cctgctttgt atgttgaaca tattctataa
    49021 ttgtaccatc tggatgtctc aatgtcatat tatatcctgt aggaactttt ttcttatctc
    49081 ttactacaac tgctccatta tccaatagat aacttctcca ttcatcaaca gaatcaacca
    49141 taaaagtaga ttttgtttct ataaatggtt taagagcatc atcagaacca gataatagta
    49201 aaacatttcc tatttgcgct aattccaacc caacttcttt ataggaaaaa cgcaaagaac
    49261 atttttcttt taaaagattt tcataaaaag gtatggcttt ttctatatct tgaatataaa
    49321 aacgtgttaa tatatcatat attttcatat tttcccccta tatatctata taatattacc
    49381 tctaaattat accactctac aaattatata acaataaatt aagcatagcc ttttgagcat
    49441 cttaagtaga ccttaacaat atattggggt tacattagaa tacataaatt aaatcacctt
    49501 gatattattt aggataaaat ttatgtagta gcagtaaatg ttcttctaat tatcaactaa
    49561 caaacgataa tctttaactt tctatataat gggtaacatc attttcgtaa gatattcact
    49621 agctggacga ttgatatcat ttaaatactg gtaatatata tcgccatgaa gtttatatcc
    49681 attgctattt gcccatgaaa ataagtcttc taaagtaggg tcttgtaatt cataaggtcc
    49741 taaatcaatt gcagttatta ttttctgtgc aggaacaatc ctttgtagaa tctcattttt
    49801 cccatctata taagtggcta caggaaaccc tacttcaaca tccaattttg ctaaatccat
    49861 gttatgaaaa catacaatcg gtgctcctga agataaaatt cctctctcct ccagatattt
    49921 agaaattttt tcaaaacttt gttttgaaaa ctctgaaaac tctaccataa agtcaattgt
    49981 ttttcgtatt gccaaaaaac agtactcaga ttgttcagct attgttatat tggatattct
    50041 agtcatttta ttcctccatt actttatttt catttctaat ctttttatac caaattatac
    50101 gaaaatttac tgtgtaacta attatttcat atgaatataa aacataacct ccttccattt
    50161 cttatatcga aagtatagca aaaataatct catcattctt gtcacacagt gctcttataa
    50221 tcagaacata cttaaaagac ttgcctaatt tttaggcaag tctaaaagaa atctttttct
    50281 ataaaaaatt tatacaaaca attaaaagat caaaatatag actaatataa aactataaat
    50341 ttactaatct gaacctccca tggctaaagt cacaagatta ctgatttata gaatttcgca
    50401 cactaaaata tgtcttacta attccccaca ggctaccccc ctagttccta cgttcttaca
    50461 taagttatct attatcaaac atcaaaattt aaacaagttt caggttcatc ttctattctt
    50521 gctattttaa acttacatgc aattaatact aaagatacta ttggatttat aatatttaat
    50581 aaagcgtatg gaccatatgt tagtgcagat acacctaatg ttgctgttat atacgccccg
    50641 catgtattcc atggtactaa tggagaggtt aaagttccac tatcttctaa agctctcgac
    50701 aacatcttag gatgtatacc tctcttttta tattccttat tatacattct tcctggtata
    50761 actatagata gatattgttc acctgctatt atattggtaa ttgtacaagt taccatagta
    50821 cataatactg ttccaaatac accctttgct aatcttagta aagaagatgc tactacttca
    50881 agcatacctg ttttttctaa aatacctcca agagataaag cgcatattgt taaggataca
    50941 gtggacatca tattcataat cccaccttta gataataaag catctacctc ctgtatttgt
    51001 gttgacgaaa catatccatt ttgtgctgca tctaatatcg tattcataga ttctccttga
    51061 aaaactactg caaacaatac tcccaataca gcacctacta taagccctgg tatagcagga
    51121 actttaaata caaccaatcc aattactatt gctggagcta ataataatac tggtgataaa
    51181 gtattaaatg tagaatctaa tgtagaccta attaattcta tttgttttat atctagtgct
    51241 tgacctgaat acttcattcc aattacacca taaattacta aacaaattaa taatgatggt
    51301 atagtcgaat atagcatata ttttatatgt gtaaacaaat ctgttcctgc cattgctgga
    51361 gctaagttag tcgtttcaga taaaggtgat agtttatctc caaagtaagc tccagatatt
    51421 atagaaccta cgattaatcc aattggcata cctaaacttt gaccaatgcc taaaagagct
    51481 actcctactg tacccattgt gctccaagat gaacctgtcg caagagatac tattgcacag
    51541 actaatgttg atgctactaa aaaaatattt ggtgataaaa tttttagtcc ccagtaaatc
    51601 atacatggaa ctactccact tactatccaa gttcctataa gtacgcctat tataattatg
    51661 attaagattg cttgcattgc catctttata ctattaaaca tacttaattc taactctttc
    51721 catttaaatc caagtctata tacagccaca agtcctgcta ctgctgctgc tcctattagt
    51781 ggtacatgag gtgatgaacc atacactaat actcctagac ttaaaaataa tatcaaacaa
    51841 gtaattggta tcattgcatc aaacagactt gcttttctac attctcgctc tttcataaaa
    51901 tagtttcctc ctaattattt aaacactaca ctataatttt ttttgaaaca tatttgctcg
    51961 tactagatga catgataccc ccataagtta ataaaaattc taaattgtca cctaatttat
    52021 aatcatattt tgtgttactt acatctagta tcatgtggtc tgaacttgcc cctaatattt
    52081 ttatatcagt gtctattgga attaaagagt caatatttat atcttgttta cctatagcta
    52141 tgattgccct ttttaagatt cccttatctt cataaacagg tttatttcta aaggcatcaa
    52201 ctcctatttc tccaatagga actgatggtt tttctttaca ttcaactact tggcatatta
    52261 atttaaatgc atcttgatat gttccattta tattttcacc gtatgccgtt tctctaccaa
    52321 gtagaatgga ctcgcctatt ctcaaattag taataccttc tggcatatta ttatttatca
    52381 acagatgaat agaacttgaa ttacctccag atactatttc tagatttatt ccaaattttt
    52441 cttccattct ttttgctata cttaccagtc tgcctatatt ttccctagat ggtataatag
    52501 ctccataaca ggttaagttt gtagctatac caattatttc aatatttttt aatcttacta
    52561 tctctcttac attactaaaa aagtcttctt cataaaagta tccttctcgt aaatcgccta
    52621 agtcaaacat aagaactatc ttatgaataa tatttttact ttcacataat tcatttaatt
    52681 tctttattgt tcctaattct gagttaaaac taatatcgca ataattgata acttcttcta
    52741 attcacttaa cattgggatt ctaatcaata tcttaggtag atttatatct tgtaaatttt
    52801 ttaagttttg tattcttgaa tctgctatat ggtctattcc ttcactacat atttcttcta
    52861 ctatttcttt ttgtgcacaa aatgatttag taacaaaaga tatcttaata tttctttcat
    52921 gacacatctg cgaaattaat tttgcattat gctttaactt ttctatatct atttctaacc
    52981 taggatacat aatctttcct cctcaaaatt caaacttttt aacaggagct taagagatgc
    53041 ttcttcatat tttttagata aaacacccat agatgccata atatagtcat tatctattaa
    53101 tatctttgtt ttttcctttg gaaatagtaa ttcattattt ttattgtatt tgagcatatt
    53161 atctaatatt tcaattctac tagggagcct agtaagtgct ccccctgtac caactatata
    53221 tcttacttct gttaaatctt tcccttctgc aattttgact tttccagttg ttccatagat
    53281 atttcttatt tttcctgaat gtcttagcat agctttaaca acagcttctt ttgtcaacct
    53341 ctcaacaaat aatatttctt cttttgattt tggtattgga ggataattct ctattactgc
    53401 atcaatatct atagatagtt cactttgtaa gttttcttta cctataactt ctactatatt
    53461 cttcatattt acatatacac ctaaatcccc ctcaacactt ctctttgcag taggttcagg
    53521 attaaccaaa attttattaa tatagtcact tccatctgta acagaatgta catcagtagt
    53581 tgctccacct acatccagag ttaataaatc tccaatattt ttatataaaa gtttagaagc
    53641 ttccattaca gctcctgggg ttggagtaat attttcatta accatttctt taatgtattt
    53701 cattcctggt gctgtagtta tgtgttcttc aaatacactt tgaatgattc ttcttgtagg
    53761 ttctatattc aataaatcta ttttagggta tacattttca gttatataaa gcttataatt
    53821 agtatcttca aatattaacc ttatttcctc ttgattttca acattaccag catatataac
    53881 tggtatgcct agattcatag aagctatcat ttctgcatta taaatagctg tatctctttc
    53941 tccataatcg actccaccag ctattaaaat tatgtttggg tttatttctt ttattttatt
    54001 taaatcagtt cttttaattc tacctgatgt tatttgacga ataacagctc cagcccctaa
    54061 agctgcttct ttagctgctt ttactgtcat atcatagact aatccatgaa cagtcatttt
    54121 taatccacca gctgcactag aagttgcaaa catatcatta tactctatat catctacaga
    54181 taatttatta gctaaatctt taatagcacc tgataagcca tttcttacat cgccaccttc
    54241 aaacactgtt gttggagctt gaccttgacc caaaaatata ggattgttag tattaatatc
    54301 gtggaaagca tttaccactg tagtagtact tccaatttca gctaccaaca catctatttt
    54361 taaagaaacc ttgctatttt ccattattta ttactcattt ctcttctttt ttcaactaag
    54421 aaagtggcta catgtatgcc tttactgttt cttccaaatc cagcatctat gccttgttca
    54481 acagctattt caggtgttac ttgtgttcct ccacacgcaa tcattacttt atctcttata
    54541 cctttttcta cacaataatc gtgtaatttt ttcatgtttt tatagtgaat atcatcatga
    54601 cttattattg tagatgcaag tattgcatct gcattcagtt caatcgctgc atctaccaat
    54661 ttttctacag gtacagaagt ccctagatag tggcattcta tgccatattt ttcaatacct
    54721 ccatgtttta tatctattat ttctcttaaa ccaactgaat gttcatcttc tccaaccgta
    54781 gctgaaacta ttttcatagg tttttcttct atatctgctc ttatttcttc atcagttagt
    54841 accttaggtt cttctggtat tactaaattg tttaaatcta tatcaaacgg aactcttcct
    54901 ttcacttcta ttctagtccc ttcagattca tgcataactt ctctacttat gacctcaatt
    54961 tcttgtaagt tcattttttt gacaaattcc aaagctgcaa actctgctgt tcttttatca
    55021 gtaggaagca tcattgttat cattacaatt ccatctgcac accattccat ttcaggctta
    55081 attttagttc catttctgta ctcatatgat tcttttagtc tattttctac attatcaaaa
    55141 tcgtcaagct catcaatata tattattttg tctggatttt caaatgtaca tccatctatt
    55201 aacattgatg gattatctat agcattttca tcatattgag ctacattatt gtatccaaag
    55261 tgagcagtta caggtgctaa ataatcttct tctctttcat aaacagtacc ttcaccaact
    55321 ccacctttta tttttctaga tattccatca ccatttcttt ctggataatt tccagaatca
    55381 acaaaaaatc cttgttccac agattcaaaa tatccaccaa cttcagatat ttcttccata
    55441 tataatacag ctctttcttt aagttctctt gctttgtctc ttaattcacc cacatcttta
    55501 agttctatca tatccataag cccatccata ccaactaaag cttgctttgc tgtatcacat
    55561 gcttctatat tgtatatatg ccaaggtaca tttctacctt catcaggtgt tatagttgat
    55621 tgtatgtcag ctctagttaa tactgatgtt aataagttta atacatgagt aactgttgct
    55681 tctctagttg atgattccat atatttagta ttcatttgag ctctcatctt ataatcacta
    55741 aataactctc taagtgcaac agcgtaaggt aagtctattt ttaaacatgg tgctggaggt
    55801 gctgttggtg gaactgttga taagcatata ttacttttat ctatacctat tttttctgaa
    55861 aatatagagt ttaaagcgtg ttgaaccata agttcaggca ttaccttcca tgcttctcta
    55921 gcagtagcat tagcattatg tgctccatca atttgagcta tattggcaaa agccattatt
    55981 ttctttgcct cacaagcatc tacaaaagac cttaccatgt ttatattcct atacagaaca
    56041 ttatattgtg ggtcttgatg tgctccattg actccttcct cagcaaacat tactgctact
    56101 tcaggaccag caacaccact tacatatgaa tggtaattaa taggtctacc aacttcttct
    56161 tctataaaat caagagcctt cctttgagct cttacttgtt ttcttgttat tggaactcca
    56221 cctatacctt gtggagttcc ttctataagc ccatcaaaat gtgattgtcc agcagttctg
    56281 ataaccatta gatggtcagc tccatgatga gctgccattc tcattcttct tatatcatcc
    56341 tcaaatctac cagaagctat ttcagtagta ataactggac ttggttgagg gtctatatta
    56401 ttgaaatact tagatgatgg tagccctaca ctcttcttta aaggttttgt acagtcttta
    56461 tatttaaaag gtcccatttc aagattttcc aagtgttctc tccaaaccca acctcttcgt
    56521 tttggtttat atttatccaa atcttttaat atatagtcta tatccaattt tttattttcc
    56581 tttaacatga ttctacccct tctctaaata aacatgtaac ttcatcccaa tattcaccct
    56641 taactaataa caaacctgct tctctaaccg ttatattttt agatttagcc aatttataaa
    56701 ctacatgccc tgctccttta cctataagac ctctttccat aaccccttct aaaataggtt
    56761 ttacttcaag agaagaaaat cccattctca acaagacaga cctttctatt gatggtgttg
    56821 tattcttttt accaagttct atcattggtt ctactatctg ggtagctaaa ctccaaaacc
    56881 tttcttttaa ctcatcatca ctcagttctt gaagatgttt tcttctaact tcaaaatcat
    56941 cctctctttt tttcatgaac attcctccat tcgtattgat ttaatcgaat tttctgaatt
    57001 tttattttat attaaattaa aaaacaataa ttactataaa ttatttaata catcaacaac
    57061 aaaatctctt gaacttttta cttcttccat taaaaattct atatctcgat tacttagctt
    57121 atcaatatta tcaatgtgat tattagatat acaatttttt ataaatgact ttcttatttt
    57181 attcatatca atatcaacac atttcaataa ttctggtctt tctggtaata ttaagtttac
    57241 tcctgcaatt tcttctcttg gatttccaaa ttttatatct attccatttt ctcttgcaaa
    57301 agtaagctgt ggacatatat gtttacctgc gcctgtatat tctgtttctt gtaccactat
    57361 aactttatcc tcatctaact cttgagctaa agagaatgct gctgctaaag atgtatttcc
    57421 tgctggacct ctttctatac cttctagctg tgccaataat tcagttgtat aaaaaacttc
    57481 tccttgtttt actaatacat atctatccat atatctaagt ggtctagctg cagacctagg
    57541 tacatctgac ctatcaggcc atgtacagta tggtatccca aaacctgtat gtcctgttgt
    57601 aaatgatttt ttattaaatt gattatcact agccatgtgt aatcctttta aatctacact
    57661 tgctcctact actttagtat taatagaacc agcttttatc aatcctcttg cagttccagt
    57721 aagattacca ccacctgcat ttgtacaaac aactatatca gggtcttttt tatacttttc
    57781 tctaaattgg atagctaact cataacctaa agtttctaca cctgcaattc caaatggtga
    57841 atataatgat gcgttaaaat agcctgtttc ttctaataaa actaagaacg tataaaataa
    57901 ctctggtcca acagttaact gaacaacttc tgctccatat gcctcacatt ttcgtgcttt
    57961 ttctataatt tcaggttgac ctacaccatt agaatcataa cattcttgaa ctattataca
    58021 ttttaatccc tgcattgccg cttgtgatgc tacagctgca ccatagttac cactagtagc
    58081 tgctattaca cctttatatc ccatttgttt ggcatgatat actgcagtag ctgctcttct
    58141 tgctttaaaa ctaccagatg cattcattgc ttcatctttt acaaatattc tagctccttt
    58201 accttcttta gcacattttc tagctaattt agtcaaattt ttaagctcta ttaaaggagt
    58261 atttccaaca gcatactgtg attgaattgc ttcaatatct tgtaatgtat atcctgtttc
    58321 actcatcatt ttctcataat caaaccctat tccctcttgt tcaaaataag aataatccaa
    58381 tccgattgca tttttcatta tttcgttatt tcttcccata acagcttgat aactcatatc
    58441 tttcattgat gttgaattat tcattatata cacctccaaa taatatttct ttagcttgga
    58501 ttcctatttg gagtaactct ggtatacatt taccataatc atgagtataa tatggattta
    58561 ttttaagtaa attacctttt acaagtctgc ctgtaattgt tttaacttct actaagtcgc
    58621 ctatagaagc atcactttgt ataaaacctt taacccacat ttctaaggat actttcttag
    58681 tatcctctgg aacttgtgga gctctttctt ctggagtcaa tactatattg tgtataataa
    58741 cccaatcatt aattttagca tccatttttt catcccctta ttaattgact attgccttac
    58801 taattaattt actgttattt taaattaaac attattttac caattagcta actgttatct
    58861 tactaattag ctagttgcta tcttactaat tagttagctg ttatcttact aattagcctt
    58921 tgattcttct tccaattcaa cttctatctg atttcttata tctcccatta ttgctctagg
    58981 tactggtata tccaacatag ttttaagacc tggtctagca tttattatta atggaataga
    59041 gtttactatc attgcataag tacctactcc tccaggtatt tcaggattta tttgtaaatc
    59101 aatgtttggt atacctttta ttgatacata gtctcctgtt ttaatacctt ctgcttctgg
    59161 tattatctgt tgtggatgct ccatttcaat taaaacttct ccatttacat atccatatcc
    59221 acattgtcta caaccagcaa catttccagc caatgcctct ccgtacttag attttctatc
    59281 tactatagtc attattggct cttttgtttg ttctatctta tctaatttcc aacctaatcc
    59341 atcagctatc atatttattg attctacaaa acctacatga cctgctatag tcttatcttc
    59401 tacacctttt ataaattctt cccttgttac tccaacacct tgttcctcca taacagactt
    59461 tccaaatgga gataaatcat ttactctctt agcttttata ctagtaactt cttcacaagt
    59521 gcctgaaagc gccaatacta gtaaatctaa tacaaatcct ggatttattc ctgttccaag
    59581 tatacttact ccattttcct ttgcaacttc atccattttt ttagctaaat ctgcatcatc
    59641 agcttgtgga tatgccattt gttctgctgt tgatataaca tttatttttc tatttaataa
    59701 gaaaattatt ttatcaaaag cctttttagt aaatgaatca gttgctaaca aaacaacatc
    59761 tacacttttt tctctaaaaa cttcttcata atttttattg ataataactt ctggtctttc
    59821 tcctctttca attcctaaaa cattatacat actttttcct gaagtacttc tactacaaac
    59881 agatactatt tctatacctt ctttctttag aatcatatta gccataccta ttcccatagc
    59941 tccaaatccc catattccta ctcttacttt tctcatatat acaccctcct aaaaatttat
    60001 atattctcaa taattagtta aagcataaat cgtgccaact ttaaaattaa agtaaatatt
    60061 tattgaaacg ttgaaactat aattatttta ttatgtattt tcataaataa aaaataatat
    60121 ttcttatgag aaataaaaaa aagagcaaaa tattttgctc ttttataata attttgctcc
    60181 tctattttgc ttttttattt tactttgatt ttgttttttt atcttgctta tttataatat
    60241 tttgatatta tattttttta ttttatactg taaattttgc ctattcatac ctaaaattga
    60301 tgcagcttta gttatattgt aagatgcctt ttctaatgat tcatatatat acttttcttc
    60361 tatctcactg ataatcttac taagtgtttt gtcctccaaa tcgtttaaag aataattgtt
    60421 actctcttta ttctctttaa gattgtaata gtcgtttaga ttaatcatac ttgtttctat
    60481 ttgtacctca tcttccatca tactcatagt acaataaatt atgttttcta gctctcttat
    60541 gttaccttgc caatcgtaat tttttaaaat tttaatagct tcatctgaaa ttcctactac
    60601 ttttttattt aactttttat tatactttga aataaatttt ttgcacaata gaggtatgtc
    60661 ctcttttcta tctctaagag gaggaatttt tatatgaatt atgttaagcc tataatataa
    60721 atcttttcgc attttatgct cgtttaaaat tacctttgga ctttcatttg tcgttgcaat
    60781 aattcttaca tctataggta tatccttagt tcctccaacc cttcttacat aaccttcttg
    60841 taaaaccctt aatagtttag cctgtaacat aataggcatg gaatttatct catccaataa
    60901 caatgtccct ttgttagctt gttcaaataa gcctggccta tctattgctc ctgtaaatcc
    60961 tcccttgcta gttccaaaaa gaataccttc gaatagtgat tccggcaaag ctgcacaatt
    61021 ttgtgctata aatggatagt ttttcctatt acttccatag tgaatggact ggctaaataa
    61081 ctctttaccc gttccagttt caccgtatat aaatactgaa gcatcagact tgcaagcttt
    61141 cttagattta gctattgctt ctttaatcaa attactttcc cctaaaatat catcaaattc
    61201 ataacccttt ttttgtctaa tactaacttt attgactaaa ttttttttat aatttctgtt
    61261 tagtaaattt tctatcgttg tcatattctt tgatatctca agtgccccta caaactcgcc
    61321 tttaatatca attggtattg tagtattaat tgctgttatc tctcttccat ttttatccag
    61381 atatttttga atattgtctt tgataacttc ccttttttcc aaaacttcta atagtgtact
    61441 cctgtttatt ttcattttgt tgactatatc aaaaaatgaa ttggttaaaa tatcgtgtct
    61501 gttcattttt tccatttttt gtacagaatt attgtagatt atagttttcc cgtctttatt
    61561 aacaaaatgt accccttcat caatattttc caatatttca ttcattattt taaaaaatat
    61621 ctcttggttc aactatatca ccccagtacc taaatatata aaaacccaaa aaattaataa
    61681 aatgttgcta ttgtatttat cttatcatat atttatgata ttt
//
LOCUS       DAMJVK010000010        58910 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.010, whole genome shotgun sequence.
ACCESSION   DAMJVK010000010 DAMJVK010000000
VERSION     DAMJVK010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 58910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58910
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.010"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            252..1328
                     /locus_tag="PC414_000655"
     CDS             252..1328
                     /locus_tag="PC414_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF2463067.1"
                     /translation="MKAQRLNAVLEQMKKDDISQMLVSDPTSIFYLTGVLIHPGERLL
                     ALYLNLNGNNKLFINELFPVSEDLGVEMVWFNDTQNPVEIITEHIDKNATMGVDKNWP
                     ARFLLNLIELGGGSKFVNSSYIIDTLRMCKDEEEKELMRIASKLNDKAMEQLKATVSG
                     ELTEKQLVGKLSKIYEDLGTDGFSFDPIIGFGPNGANPHGEPGNALVKPGDAIILDIG
                     CIKDNYCADMTRTVFYKEIPEKGREIFEIVLEANKRAEAIVKPGVRFCDIDAAARDYI
                     TEKGYGQYFTHRTGHSIGLEVHDKGDVSSINTDTVQPGMIFSIEPGIYLPGEFGVRIE
                     DLVLVTEDGCEILNKHDKEICVVG"
     gene            complement(1431..2273)
                     /locus_tag="PC414_000656"
     CDS             complement(1431..2273)
                     /locus_tag="PC414_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDF2463068.1"
                     /translation="MDKIKLIVDSACDLPDDIIEKYNIEVVGLNVSFGEESYISGKEI
                     DNETFYRKMSESKVLPKTSCPSPDKFLEAYHCQEESVLVINISSGLSGTYNSALLAKD
                     MFEKEGNNKKVEVIDSLSGSIGIGQLVLKAAILIKEGKEIEEIVSIINKYKDNPPFFG
                     TLETLENAIKGGRINPIAGKIINTLNFKAIIQVADGVVTPIDKARGEGNSLKKLITLV
                     ESKIKDKEEKVLFIGHANCPEKAQKVREVMEKDVKYKDVVICEIGSVMGTYTSKGAIL
                     ITAI"
     gene            2457..3029
                     /locus_tag="PC414_000657"
     CDS             2457..3029
                     /locus_tag="PC414_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2463069.1"
                     /translation="MKTNNMKQAILSSATNLIAKNGVQNTSLADIAKDVNISKGTLYY
                     HYASKDDIIYDIADTHLEVITSAILNCVKNVKSKNSQIEMVNLILEKISTIESRGRVH
                     MYLICEAITSNNDLKERIRLKYIEWRTTLKSEIVESLEKYNSSENEQDAESFSFLLMS
                     IVDGLVVQSLLKTEKIPYENIASFLVNHWI"
     gene            complement(3192..3464)
                     /locus_tag="PC414_000658"
     CDS             complement(3192..3464)
                     /locus_tag="PC414_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463070.1"
                     /translation="MLRERFVVRNHHNPHTSGCCGTGGEGCCGGDNHEHSHQHSHSHG
                     HEHEHVNGVGHDHNHDDGFSGTHEHSHEHTHTHGHEHSDGHTHEHK"
     gene            complement(3591..4463)
                     /locus_tag="PC414_000659"
     CDS             complement(3591..4463)
                     /locus_tag="PC414_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF2463071.1"
                     /translation="MYGESKKLSIDNNIFLQYCLIILGMILSTLGINLYLAPAKLLSG
                     GVAGICVILYKLFGINQGISSFLMNIPIFIIARKYFDNKFLLISFVNMLLFSLALGLT
                     QDIAKYFPIDDTMLQCIYGGALTGIGMGLTFKARATAGGLDIIAAIMKRKYDIPMKNT
                     FLFINFFVVCAGAFLFGAKLVMYTLITMYIISFTMDIGKDCFDRKKSILLISNKYDEI
                     SKVIMNKMGRGVTFLEAEGAYTQNKKKMIYCIISANEIGKFKDLIYDMDSEAFISVNN
                     VEEVKGGGFKDKFL"
     gene            complement(4759..7866)
                     /locus_tag="PC414_000660"
     CDS             complement(4759..7866)
                     /locus_tag="PC414_000660"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDF2463072.1"
                     /translation="MAKFKPLVDSSVKQAEAQVFDYWKDINILEKTLEKGKDDPSFVF
                     YEGPPTANGNPGVHHVLSRTLKDSVCRYKTMSGYQVKRKAGWDTHGLPVEIQVEKELG
                     LTSKQQIEEYGIAEFNQKCRESVFSFEKQWRIMTERMAYEVDLDNPYITLDNNYIESV
                     WWILNKFNKEGYIYEGHKILPYCPRCGTGLASHEVAQGYKEVKTNTVIAKFKKKDADE
                     YFLAWTTTPWTLPSNVSLTVNADVDYLKVKKGDEVYYVSKPLADKVLGEDYEVLEEMK
                     GKDLEGLEYEQLMPFVEVDKKAFFITVGDYVTTEDGTGIVHTAPAFGEDDYNTGKRYD
                     LPVIQPVDETGKFTSTPWEGRFVMEDGLDVEIIKWLASENKLYSKEKVVHNYPHCWRC
                     QTPLVYYAKPSWYIEMTKLKDKLIENNNGVKWFPSFVGEKRFGNWLENLNDWAISRTR
                     YWGTPLPIWRCECGHTDSVGSRAELAEKAIEDVNPETVELHRPYVDDIHLKCEKCGKP
                     MTRVTEVIDCWFDSGAMPFAQHHYPFENKENFDQLFPADYISEGIDQTRGWFYSLLAV
                     STFVLGKAPYKSVLVPDLVLDKDGKKMSKSRGNTVNPMELFDQYGADALRWYLLYVSP
                     PWTPTKFDMDGLKEIQSKFIGTMKNVYNFFTLYANTDDINPTEFFVEYKDRPELDRWI
                     LSKFNNLMKDVEENLAIFELNKTLRMIQDFINDDLSNWYIRRSRRRFWATELTEDKKA
                     VYNTTYELLHGLCRAIAPFAPYMSEEMYRNLTGEVSVHLAEYPKCNEELVDTKLEEKM
                     DLAKNLVTLGRASREVERIKVRQPLQKVLVDGKFEDTISDVVDLIKEELNVKEVIFAK
                     DLDEYMNFSLKPNFKEAGPLLGSKMNLFVGALSKLNAHETANKLEKGETLTVDLDGEA
                     FEFNKDLVLIGITAKEGFNVSVENNLFVILDTKLTEELINEGYAREFISKVQQLRKSN
                     NFEVLDNIVIEYCGDDEIAKAVDHFNEYIKSETLALEINRVDDKSLEEQNLNDHMTGI
                     KVIKK"
     misc_binding    complement(7959..8191)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8366..8893)
                     /locus_tag="PC414_000661"
     CDS             complement(8366..8893)
                     /locus_tag="PC414_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HDF2463073.1"
                     /translation="MLTPIEIENKEFKKGLRGYRDEEVDEFLDIIKEDYESLCRENTA
                     LKEKLGLYQDQVNKYENIEETLKATLITAQSAAEDTCSAANKKAKIIVEEADLKARQI
                     IEQANNRVIEIRKEYDALVKEFKIFRNKFKSLLEDEIRSVDEIFYDVDEKCVNAFEGT
                     TVYNYSNDEAATTLE"
     gene            complement(8928..9713)
                     /locus_tag="PC414_000662"
     CDS             complement(8928..9713)
                     /locus_tag="PC414_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HDF2463074.1"
                     /translation="MDKLKLTNHIKDIDLKNKMFKVIDKANSCIKNYDVKSTEFLNPY
                     EVKNAVAILNSTNEIKYSVDGGYEQAERSTVFIYPFYMEYEDIEDTLRFLQIEGNFKF
                     KNISHKDYLGSILGLGIKREKIGDIIIHDSFCQVVVSSDICDFIIVNLEKVSRNNVIV
                     KEISRENIVNSSSKYKEVSFTVSSDRLDCVISGIYNISRQDSAKYINGEKVHVNYEKI
                     TSTSKIVKNDDLISIRGKGRAKVTQIGDITKKGKIKVQARLIV"
     gene            complement(9723..9980)
                     /locus_tag="PC414_000663"
     CDS             complement(9723..9980)
                     /locus_tag="PC414_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HDF2463075.1"
                     /translation="MGTIRIALYYLLDIIAWMIVIKSLMTWFPNGRQSKIFDILENLT
                     EPIEGPIRSIMYKYTNGPIDFSPLIAIVLLMFLRQLVLVIF"
     gene            complement(9999..10457)
                     /locus_tag="PC414_000664"
     CDS             complement(9999..10457)
                     /locus_tag="PC414_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HDF2463076.1"
                     /translation="MSNGIISKFKNWIVDEEEDYIEDEYESGMDDIVQEEEMNSGFST
                     AKANKIVNLHTTSQMKVVIVEPKVYDEAATIADHLKQRRAVIVNLEGLTNSEVRKSIF
                     NFMNGAVYVLDGSIQKVSKSIFILAPNNVDIDANMKKELESKAFFPWQNK"
     gene            complement(10497..11201)
                     /locus_tag="PC414_000665"
     CDS             complement(10497..11201)
                     /locus_tag="PC414_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HDF2463077.1"
                     /translation="MGSIKDNLSEIRNKIDKAAKKVDRDSKEITLLAVTKTVDVDLVN
                     EAMREGITSVGENKPQELARKYDIIGDKLNWHLIGTLQTNKVKYIIDKVCMIHSLDRI
                     ALCEEIQKRAEQINREIDCLVQVNISKEESKHGLDREEVVDFVKEVSEKYKNIKIKGL
                     MTMAPFIEDEDEIRGVFKGLKDLSKQIKELNIPNVGMDVLSMGMSHDYEIAIEEGATI
                     VRVGTSIFGERNYNKK"
     gene            complement(11211..11846)
                     /locus_tag="PC414_000666"
     CDS             complement(11211..11846)
                     /locus_tag="PC414_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463078.1"
                     /translation="MKKVKYTRLVILGILIYLVFNILVMIISKNVDTLVLKNEKVDAK
                     IHVKGTIEKDANDQSNYLSTYISKEDSNNFEKNQNVIIEYHEQKLDAKVYKIYENNNK
                     IMVKLKISNQIVANQDTSVEEFDIIYNQMECLKIPKTSIKTKDNKRGVYVIDEQSQSV
                     KFVILEGITYENESSVFVDYYKNDINGVTSVNLYDKIILRPNSINTNIRIK"
     gene            12227..13267
                     /locus_tag="PC414_000667"
     CDS             12227..13267
                     /locus_tag="PC414_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2463079.1"
                     /translation="MSEILLGTGLLLLVLCCFTIFSYKAPYGMKAMGALANAACATFL
                     VEAFNLSLLGDVLGMEFFRSVGAANGSLGGVAAAILVPLALGVSPVYAVLIGLSCLDF
                     SILSGFVAGYFVSFLIKFIEKKVPPGVDLVVVILLCAPLSRLIASNVDPLVNSTLLQI
                     GQVLIEATNISPIIMGIILGGLITVVGTSPLSSMALTSIIGLTGLPMAIGALGAFGSA
                     FMNSTLFAKLKLGSKKDIISVAIEPLTQSDIISANPIPIYSTNFFGGAFSGIIVALMG
                     LVNMTPGTATPVAGFAIMFAYNPVQQVLITAGLCALFNVIAGFLGSKIFSTFKVLTAG
                     EIRGTDVEEEIA"
     gene            complement(13730..15085)
                     /locus_tag="PC414_000668"
     CDS             complement(13730..15085)
                     /locus_tag="PC414_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009170073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HDF2463080.1"
                     /translation="MNVGNILETIKMIDEEQLDIRTITMGISLLDCIDPDGDKAREKI
                     YNKIMSSAKDLVKVGKDIEREFGIPIVNKRVSVTPISIIAGATDENDYVKFAQTLDKA
                     AEDLGIDFIGGFSALVQKGYTKGDKILIKSIPKALASTNKVCASVNVGSTRCGINMDA
                     VKEMGEIIKETAELTKDAKGFGCAKLVVFCNAVEDNPFMAGAFHGVGEADRIINVGVS
                     GPGVVKRALEKVKGEPFDVVSETVKKTAFKITRVGQLVAKEASSRLDVPFGIIDLSLA
                     PTPAIGDSVANILEEMGLEVVGTHGTTAALALLNDAVKKGGVMACSHVGGLSGAFIPV
                     SEDAGMIDAVIKGALSIDKLEAMTAICSVGLDMIAVPGNTTAGTLGAMIADEAAIGMI
                     NNKTTAVRIIPAPGCDVGDMVEFGGLLGRAPVMPINTNSSELFTQRGGRIPAPIHSFK
                     N"
     gene            complement(15111..15380)
                     /locus_tag="PC414_000669"
     CDS             complement(15111..15380)
                     /locus_tag="PC414_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HDF2463081.1"
                     /translation="MKAILTVIGKDKPGIVAGISDELYKQNINIVDISQKILQDYFTM
                     IMIVDLEKSLNSFEKTVEDLVERGKKLSVDVKIQREEIFNSMHNL"
     gene            complement(15670..17625)
                     /locus_tag="PC414_000670"
     CDS             complement(15670..17625)
                     /locus_tag="PC414_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463082.1"
                     /translation="MKKYEIFIFMSIIVLCFISFPHKVFGQEENCLEDFERWVQENKN
                     NKEEIVYTLSCDMVIDEEFRFYIPYDSNFTIDTNKYKILIKNHGRFIIDDNELNIIGE
                     GGKEGVIHIENGGSISIGINNIIATDGTALYVEEGGDLHIKSSYESVEKIKANGKDAI
                     GIYTENDIRILNKDIEVNGEHAIGVYSKGDVEIEETSIKAYSSNNESGLLKDNNKLAQ
                     SIISESKKVYIIDDYNELIPSIAEDSGYDIVKCCHRGIGVFSDEIKVSKDDKIENIKF
                     PKYMVLETSSGNRLDIDVEWDFTDYYEKLEKGENFNITGKFKTEMLNKEKIIINDDVV
                     PILNVSVIEKKPIDNLELEFQNTKNGYVAVLFYDMPYSASKVFVEYSSDGINWISEEQ
                     EDIRDQAILFFEDFKLRCFRVKVVGGLKEGYSNIVFKPGFIMGGGDNQETPDDEREND
                     DIDGDRGGGGRDDPDRDEDDNNQDGNNGNENNDSNNNQNNNNENNNNENDNSDEKQPN
                     TPKDDVESSTDSNENKNPHDIPKDGNKENNSDKEKGFYNKNSITENDDNYDLSSKLVF
                     GTNEHSFPYRESISNKNQDTNKNKSNISDYDGIINNNYKLESKEYNLKKDIIFLFCIL
                     TPIMIGSILIVNPDTRKSIIKFIKLKK"
     gene            complement(17808..19283)
                     /gene="spoIVA"
                     /locus_tag="PC414_000671"
     CDS             complement(17808..19283)
                     /gene="spoIVA"
                     /locus_tag="PC414_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HDF2463083.1"
                     /translation="MNNNIYEDISKRTQGDIYIGVVGPVRTGKSTFIRKFMEKLVIPN
                     IDNEFKKDRTRDEIPQSGSGKTIMTVEPKFVPADGVEIKIKDTVSLKVRMVDCVGYIV
                     EGALGHEEGGKQRLVSTPWSQEAMTFEKAAEIGTKKVIKDHSTIGIVVLTDGSVTGID
                     RKSYVEPEERVIQELKNLKKPFAVVLNTLSPKSEETSMLRSELEEKYEVPVLPMNVVE
                     MEEEDIEEVMEAVLYDFPLTEIRINLPKWVEGLERNHWIKSSIITTLKQSIIDIGKIR
                     DIEGIIQGFSELEFLEDTGVDNVELGEGVINIDLQTKQDLFYNVLEEKSGFKIEGDYQ
                     LLSLITRLSKVKNEYDKIESALIDAKIKGYGVVAPSLEELSLEEPEIMKQGKQYGIKL
                     KANAPSLHIIKADISTEVSPIVGNQNQGEEMIKYLMEVFEEQPADLWESNMFGKSLHD
                     LVKEQLQSKLYTMPEEIRVKMQKTLQKIVNEGSSNIITILL"
     gene            complement(19455..20750)
                     /locus_tag="PC414_000672"
     CDS             complement(19455..20750)
                     /locus_tag="PC414_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2463084.1"
                     /translation="MSVEVNKPSIWKNFKVLIILFVAGAFIYALPYLKNYYYDTFAQA
                     FNLTNTQMGSLGSIYGLLAMVSYLFGGFLADRISARKLLSGSLIITGISGLVLTLYPP
                     YIVVFLIHALWGITTILTFWPALVKAVRMLASENEQGKAFGFMESGRGITNAIHLSLV
                     LILFGYISSKVSDESGLTAIIVLYSAINVIVGVLVFFKLKDNEKEVSAKFDKEAFFAV
                     IKMPHTWIITIIMFCSYSANMSFYYFTPYSTEAFGATVVFASAISILAQYCRPVASAA
                     SGLLGDKVGSSKIISYGFILMIAGLLGVIFTPTKSSMIYILLIGCVAIYVSMYAIQGL
                     HYSLLEEGNYSLSISGTAIGIVATLGYLPEVLCPLAAGKILDAFPGVTGYRYYFIVLT
                     IVAIIGLVFTFIWMNMTKERCKELIEMNNNSNENTTKVS"
     gene            complement(20934..22343)
                     /locus_tag="PC414_000673"
     CDS             complement(20934..22343)
                     /locus_tag="PC414_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF2463085.1"
                     /translation="MKKKIGFWDLVFMNISALFGIRWIAKSTASSFGLGLGSIPVWLL
                     FAFIFFVPASLICAELAATYPKDGGLGEWVKQAYGEKWGFMVSWLNWTSKIFWYSSFL
                     TFLAINIAYMLGNPDLSNNKMFILALSLIIFWLLSLVSTRGMAFGKFFTNTGALGSTI
                     PAILLIVMAFMSVVILKKAPSASIYTIQNIIPKIDANSLVSISAIIFALSGAETTANF
                     ITEMDNAKKNFPKAILTVAVLIGGIYILGSVAITMILPPDKIAASTGILDALAKVAQD
                     LGIGSWFIRIVAFGITLSVLGALILYIAGPVKMLFGNVREGVFPKQLTVTNKYNIPSN
                     AVIVQAIIVSLLLVGTNLLPSVDNIYNVLVTMTALTALFPYVLLFTSYIRLRKTRPNE
                     ERPYSIAKSDSVCINIARMVLVVTVVGILFSTAPVMETLKDNIIYEIEMIGGGLFVII
                     TGLMLWKNYEKKISNNSGC"
     gene            complement(22395..23534)
                     /locus_tag="PC414_000674"
     CDS             complement(22395..23534)
                     /locus_tag="PC414_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF2463086.1"
                     /translation="MSNNMRLERLRKYMNEKSIEIALIFEPDNQFYISGFKAITYSRP
                     IVTVVTQDKIELIVPGLEELHAKEVAKVDNVYVYYEIPEMKEHGISHKHYLDIILNKY
                     PKGTTVGIEKDIVSASFSEYITDKSFAIKDVGSKIFEMRYVKDAKEIEFLKIAGYLSD
                     IGIKGSLENVRVGMSELEFDVAGDNALLKYVSENYPDTYIGFANWTCSGIDRTAQPHL
                     DSNTRILQRGDIVIHSRQVWYENYRAENERTFIIGKPTERQKEVFKIAVEAQQAGLDT
                     IKAGIPARMVDEAARAVVAKYGLELYSNHRIGHGLGLSEHEEPYLRFDNELILEEGMV
                     FSMEPGIYIPGVGGFRHSDTAIVGKNGATIITNYPRSVEELILDI"
     gene            23853..24503
                     /locus_tag="PC414_000675"
     CDS             23853..24503
                     /locus_tag="PC414_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2463087.1"
                     /translation="MTNKELQKKRILMYFIEAAQNIMENEGIENITLRKVADMAGYNS
                     STLYKYFKNLDHLISFASIKYFKDYNLNISRCIENVNDEYKKYIIMWKLFCKHSFDNA
                     QAFYQVFFNLSSDELSYITKQYYDMFPEDLGIHDSDISLMITGESIKERNKILLNNLV
                     DTGYIPAKDLDIINDIIISFYQNLLFLKKNTGKNSDDDELTMKMISSIEYIIKHAK"
     gene            complement(24660..25679)
                     /locus_tag="PC414_000676"
     CDS             complement(24660..25679)
                     /locus_tag="PC414_000676"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDF2463088.1"
                     /translation="MEKVCVLGTGSWGSALALGLAKKGNDVSMWTRKEEQAKKINRTK
                     ENTDYLPGVLFPNNITISTDIEKTIKDCKVIVLAVPSQAVRSTCQKIKPFIKEGQVIV
                     NVAKGLEKGTGLRLSQVCEEELPQNPYVILSGPSHAEEVARDIPTTVVVASKDLKIAQ
                     MIQDLFMSPKLRVYTNPDIVGVELGGALKNIIAFGAGICDGLGYGDNAKAALMTRGIS
                     EMSRLGIAMGANMSTFAGLSGIGDLIVTCTSMHSRNRRAGILIGKGMSLEDTLKEVKM
                     VVEGITATEVAHDVAEKLDIDMPITNAIYSVIKKGSNPKEVGIELMMRSKKHEMEEVV
                     LGDDI"
     gene            complement(25710..26324)
                     /gene="plsY"
                     /locus_tag="PC414_000677"
     CDS             complement(25710..26324)
                     /gene="plsY"
                     /locus_tag="PC414_000677"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HDF2463089.1"
                     /translation="MEIFSYIIIAVVAYLLGNISTSYIVAKRIAGVDIRTQGSGNAGS
                     TNVLRTLGKRAGAMTFLGDVLKGVMAVLISEFAARLVGIDTLLAGYLAVICVVAGHNW
                     PAVLGFRGGKGVATSLGAMLAVNPVITLMCLAVFILVVAITKYVSLGSVVGIGCSPIF
                     MIMVKNKAGLIVALFLTASVIYNHRANIKRLLNGTERKIGQKKE"
     gene            complement(26359..27678)
                     /gene="der"
                     /locus_tag="PC414_000678"
     CDS             complement(26359..27678)
                     /gene="der"
                     /locus_tag="PC414_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HDF2463090.1"
                     /translation="MSRPVVAVVGRPNVGKSTIFNKFAGKRISIVENTPGVTRDRIFA
                     EVEWLDKYFTLVDTGGIEPDSEDIILSQMRNQAMLAMDMSHVILFIVDGKAGITAADK
                     EIAQLLRKTKKPVILVVNKIDSQSQFDNIYDFYELGFGTPFAVSGANSMGFGDLLDEI
                     VENFPAGLDTEYEEDIIRVAITGKPNAGKSSILNKILGEERVIVSPIAGTTRDAIDTY
                     FEKNGQKFLLIDTAGLRRKSKIYETIEKYSVIRAMSAVDRADVVLIVIDALEGVTEQD
                     TKVAGIAHDEGKGCIFVINKWDLIEKDNKTMSNYTKDIKEKFPFMMYAPIVFVSAKTN
                     QRMNKILDTVEYVSNEHSKRISTSALNEVIGEAVMLNQPPSDKGRRLKIYYGTQTDIR
                     PPKITLFINDKDLTHFSYQRYLENKIRENFGFEGTSIKFEYRQKNKK"
     gene            complement(27712..29040)
                     /locus_tag="PC414_000679"
     CDS             complement(27712..29040)
                     /locus_tag="PC414_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HDF2463091.1"
                     /translation="MEVNVKKVNNIISKVYKDSIAEEMGIEVGDLLISVNEQPIHDII
                     EYRFLLSDEYLDVEIQKKDGEVYIYEIEKDYDEDLGVEFTNPIIDQAKSCRNKCMFCF
                     IDQLPEGMRETLYFKDDDSRLSFLQGNFVTLTNMSEEDINNIIKYRISPINISVHTTN
                     PELRQKMISNKFAGKLYGIMKRLADAHIEMNCQIVLCPGINDGKELDRTIKELAQLYP
                     YVNSVAIVPVGITKHRENLVELNIFNDKSASKTIEQIHQIQQKYLEKLGTRFAFLSDE
                     FYILSNSELPGYEEYEGFLQFEDGVGMIRKLKTEIEEYLNILPEKILKREKKVSIATG
                     HSAYEFIQSMADAMMDKFKNLQVNVYEIKNKFFGETITVSGLLTAKDLKEQLEDKELG
                     EALYITRSMLKADEEIFLDNIELNQLEELMRIKIIPCLNEGKDFVDKILK"
     gene            complement(29278..29478)
                     /locus_tag="PC414_000680"
     CDS             complement(29278..29478)
                     /locus_tag="PC414_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463092.1"
                     /translation="MGMDIGLNEYTLAIVTLDKNMKNGGGCPIFYANDEKDLQNKALL
                     MSKCVGGMVHDMTNGTLIIVKH"
     gene            complement(29480..30412)
                     /locus_tag="PC414_000681"
     CDS             complement(29480..30412)
                     /locus_tag="PC414_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HDF2463093.1"
                     /translation="MDRVFFVAVIIGITSRIIMLNLDQKQYPTQPNVLLSQLILAFVA
                     SSLGALLVPALINRSYTSITFLSLAAEQFRQVRANRRNTLQNLEEVQLVKRGAAFIEE
                     IARTYEVRNYMCIVTSFLTIGLYYVILAETKISEGMSLVISSICGLVLAFILKKLLTR
                     KSIGDIADVMPAEISFVDESILKVGDLKGITNIGLKSDREVYINRGVGIQIIPKNKDY
                     ANAGILFDSGQRQAIVYNIYSRLGLYGDYGEPSFVPLPRRNKEDESIMIAYLPVDKNV
                     EKVMEAVKSCPILSSSKGKNLSLKNYKIGKKGSA"
     gene            complement(30457..31389)
                     /locus_tag="PC414_000682"
     CDS             complement(30457..31389)
                     /locus_tag="PC414_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HDF2463094.1"
                     /translation="MEESLLNANLFRHSFIVALVVGILCRGLVLRVTDKQYPSRPQDY
                     LEQIIISGLSASLGAIALPALIDKEFAALTFFAVAIQQFQGLAEQERITLKNIDNEEL
                     VPKGDAYVEEIASTYESRSYISLFSALVSSIVYIVFARKYGLSFFYCTILAIVSGAIV
                     GLIFRRFLRRNSIADIADIVPAKISFEGPILMVNGVIITNIGLESTREKYRNECLAIE
                     VIPRDLGAFGIVNDIGQRQAIIHNLFIHMGIDRDVDEVDIVAISKTNLEKSTVVIPYM
                     PILKDIDVMIDVVKSTPIIETSKGKQSDFSRKTL"
     gene            complement(31444..32733)
                     /locus_tag="PC414_000683"
     CDS             complement(31444..32733)
                     /locus_tag="PC414_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2463095.1"
                     /translation="MEDLNSIYFFIVMLSIAIVILAVRYTITLRKYLKEFIYVSKKVS
                     NNEYHARLNISAKGELGELARNFNHMIKNMDKTLEEVQYKHLQLRSILKSISHGILAI
                     DVNGNILLINDEAKEMIKSEPNQLVEGHNINVVIKEEEILKQILQFIGSKDNETANIT
                     TKDDIVYKIKVDPVYLQNTNNVIIGSIVNIEDITKKVKLENMRSDFVANVSHELKTPL
                     TSISGFVETLKSNENIDVNTRNRFLEIIESESDRLKRLIDDILLLSFVENKDTLLMEN
                     VVVYDVFKEVYEITQHSASEKNIEVLYKCDDKSISILSNKDYIKQVFLNLVDNAIKYT
                     QDNGIVQVVVLKDEEKIVIKVIDNGVGIPKEDVERIFERFYRVDKARSRDVGGTGLGL
                     AITKHIVKSLNGCIKVKSELGIGSEFIVTILQKNFLK"
     gene            complement(32762..33454)
                     /locus_tag="PC414_000684"
     CDS             complement(32762..33454)
                     /locus_tag="PC414_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2463096.1"
                     /translation="MNTKVLVIDDEMHIVELLKFNLEVSNYEVSYSYDGFDGFIKAKE
                     IKPDLILLDWMLPNISGIEVLRKIRSDKDLKNIPVIMLTAKNMENDKVEGLEIGADDY
                     ITKPFSIKELLARISSVLRRYNLTSLGEENNILTTGNLKLDLSKHEVTKGSEKIELTL
                     KEFELLKLLIQNKGKVLSRNYLLDKIWGYEYYGETRTVDVHIRYLRKKIEDEDKSEKY
                     IETIRGVGYKID"
     gene            complement(33469..34230)
                     /gene="pgeF"
                     /locus_tag="PC414_000685"
     CDS             complement(33469..34230)
                     /gene="pgeF"
                     /locus_tag="PC414_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HDF2463097.1"
                     /translation="MMKDYINIKNVQDIKEYVNLSGNCEELDEKLNSVNLVITCKNID
                     AKNREDMLKVCKECDLTFENLTSNSQIHSDIVNIVNKDTIGKRRDGDALITNLEKVPL
                     LLFTADCVPISIIDAKNKAIGLAHAGWRGTFSNIGHKTIKLMSECYKTVPEDLVCIIG
                     PSIGPCCYEVSEDLVEKFNTILTNNDEKFYIIKEGSYYLDLWKINEYMLRCSGVKKEN
                     IINLNLCTSCRADKFHSYRKHNKASERIGTVLQIK"
     gene            complement(34227..34688)
                     /gene="nrdR"
                     /locus_tag="PC414_000686"
     CDS             complement(34227..34688)
                     /gene="nrdR"
                     /locus_tag="PC414_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HDF2463098.1"
                     /translation="MQCPYCNYKESKVIDSRHTDLKSIRRRRECESCKKRFTTYEKIE
                     TTPLMVIKKDNSREYFDREKIKYGLLKACEKRPVSIDEIESIVVHIENEINKCFIEEI
                     ETKKIGEMVMDKLKELDEVAYVRFASVYRQFKDINTFVNELKSILIEKGDK"
     gene            complement(34710..34973)
                     /locus_tag="PC414_000687"
     CDS             complement(34710..34973)
                     /locus_tag="PC414_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HDF2463099.1"
                     /translation="MIRVSDIMEKEIINVKNGKRMGFIIDIDMDIHEGKVVSFTIFGD
                     GSRSFFSRGGDEEVIFWNDILKIGCDTIIVNIGSELSLDEFEI"
     gene            complement(35077..35850)
                     /gene="sigG"
                     /locus_tag="PC414_000688"
     CDS             complement(35077..35850)
                     /gene="sigG"
                     /locus_tag="PC414_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HDF2463100.1"
                     /translation="MQVNKVEICGVNTSELPVLKNKQMKELLLQIKNGDEEARQQFVR
                     GNLRLVLSVIKKFNNRGENIDDLFQIGCIGLIKAIDNFDLSQNVRFSTYAVPMIIGEI
                     RRYLRDNNPIRVSRSLKDIAYKALQVRERLIRTNSKEPTVSEIAKELELEVESVVMAL
                     DAIQDPISLFDPVYQDNGDAIFVMDQVQDKKDTDENWLQEISLKEAIKKLNSREKLVL
                     DLRFYKGRTQIEVADEIGISQAQVSRIEKNALKNMRKYV"
     gene            complement(35942..36664)
                     /gene="sigE"
                     /locus_tag="PC414_000689"
     CDS             complement(35942..36664)
                     /gene="sigE"
                     /locus_tag="PC414_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HDF2463101.1"
                     /translation="MLRLKERIISFITMLGIKLKIIKPKGIYYMGGANILPPPLKPEE
                     EMELLQKLETDESVKSILIERNLRLVVYISRKFENTGIDVEDLISIGTIGLIKAVNTF
                     KLNKNIKLATYASRCIENEILMYLRKNNKKKTEVSFDEPLNIDLDGNELLLSDVLGTE
                     NDEIYKIIEEEIDRDLLVMALDRLSDREKQIMELRFGLIDKGIEKTQKEVAGMLGISQ
                     SYISRLEKKIISRLQKEMKKFV"
     gene            complement(36689..37561)
                     /locus_tag="PC414_000690"
     CDS             complement(36689..37561)
                     /locus_tag="PC414_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HDF2463102.1"
                     /translation="MFKGFKGGYMVYIEYYVIENLVINYIIISCTSILTKRYNPKKNK
                     LLGASLGGAYSVAYLYPSMNILFTVPFKIIIMSIITLISFRSKSKKDYVHVALVFYLV
                     NIFISGSTYFIIYFTGISHLKISFLIACTYVSCELLKYIYKDLKMLKYIKELTKTINI
                     NLLEKSFCCKALVDSGNLLKDPISQSDVIIVKSSVLEGFIPEKLLNINYEDIDMKKAQ
                     EIIGSLDDKTSGRVRIIPYKHAGSNKTSIIIGLKADYIEVDEQKIGNIILGISNFNDR
                     EYGAIMNPNILPGF"
     gene            complement(37851..39125)
                     /locus_tag="PC414_000691"
     CDS             complement(37851..39125)
                     /locus_tag="PC414_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDF2463103.1"
                     /translation="MIKLKKVLSLVMVGILSVSILAGCSKKEDSKGKEGKEKLVVWSA
                     PLTDHDADAWKPIFEKFEKENNCEIEFQIVPWDNYAEKYATAISAGEGPDIGYMYAEM
                     FPQFIEMGAVEDLTPYLEKSGTSDNYLYLDDAKMMGGIYGLPIEAANPGVLYYNKDIL
                     EKLGEKPPKTWDDFKRICEKATKDTDGDGKIDQWGLAQGWGSKVFGSLNWNWYPYLWQ
                     AGGDIYNDDLKSVKFNNAEGLKAANFINDMKKYIPEDALSKDSNEMIESVFGPGKAAF
                     TIMLSSAATSTFDKSFPDLNWGYVTSLEDKTVGSFASVDDLTLMSACKNKELGFKLLE
                     YMLSKESMEAFHKEIPRAPVAKGEKYQGDPRFEEMVTRDKHMYRPLTVGPHGVEIYEY
                     LWKQLQMMIAGDITPEQALNESAKYANGLLAQ"
     gene            complement(39409..40566)
                     /gene="ftsZ"
                     /locus_tag="PC414_000692"
     CDS             complement(39409..40566)
                     /gene="ftsZ"
                     /locus_tag="PC414_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDF2463104.1"
                     /translation="MLNFDVELEECAQIKVIGVGGGGNNAVNRMVEAQLKGVEFISVN
                     TDKQALYTSKAEYKVQIGEKLTRGLGAGANPEVGKRAAEESKDEIVKLLQGADMVFVT
                     AGMGGGTGTGAAPVVAGLAKEMGILTVGVVTKPFAFEGKIRMKNAEGGIAELKSKVDT
                     LITIPNDRLLQIVQKNTSMLDAFAVADDVLKQGIQSISDLIAVEGLINLDFADVTTIM
                     KDKGLAHMGIGSASGETRAIDAARQAIQSPLLETSIQGAKGVLLNVTGGPNLGLFEVN
                     EASTLVMESCDPEANVIFGASIKEDLGDEIMITVIATGFEGLQNGALDLDTKPKSSIR
                     SSLNTTVKQAVKEIEEEVIAEEKIEPPKKASIIEEDDDESMEIPTFLRRRR"
     gene            complement(40756..41109)
                     /locus_tag="PC414_000693"
     CDS             complement(40756..41109)
                     /locus_tag="PC414_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HDF2463105.1"
                     /translation="MLWVLGGLAFGVVVGYYIPFTYSMDYSIYMSVAILAIMDSIFGA
                     IKSNFEDNYDNVIFITGFIGNAILAILLTYIGEKLGVSLYYVAVLVFGMRLFNNLSII
                     RRYIISNIMNKKRKN"
     gene            complement(41208..41882)
                     /locus_tag="PC414_000694"
     CDS             complement(41208..41882)
                     /locus_tag="PC414_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HDF2463106.1"
                     /translation="MKKKIVILGAFILGVFISTYIKSLDPNRVYITLGQTESIESEVE
                     NTKKEIKNLKETLKNNKKTLNEYKEAKKNGNESIQTLMQKELEEVKELSGYSTVTGPG
                     ITITMRDSERELKDGQNPNDLIIHDIDILRVLNDLKKAGARAISINGERVLATSKIKC
                     SGATITVNDTTYGQPFIIKAIGDIDLLSAAINSPESYAKSLKDIYGINIKVQENNGNK
                     VDLFAK"
     gene            complement(41887..42585)
                     /locus_tag="PC414_000695"
     CDS             complement(41887..42585)
                     /locus_tag="PC414_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HDF2463107.1"
                     /translation="MKKRFINKSIIIGSIVLGVLVSLQLKIINIENRGMTTFKRGEQL
                     TEELKSLKKEKENLQSEVNSVKKDIETYRGKNNEGEQDILKSEIEKYETLAGYTDVEG
                     TGIKIEINSTKDLGNSDKNDSILYNYDLLLSMINKLNSAQASAISINDQRIVSNTYMY
                     LKEDKLYMNDTVIKEPIIIKAIGDSDTLASALNIKYGIVWEIETYYNAKVKVTKEDNI
                     EINGHSDINKAEGK"
     gene            complement(42608..43348)
                     /locus_tag="PC414_000696"
     CDS             complement(42608..43348)
                     /locus_tag="PC414_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HDF2463108.1"
                     /translation="MKKRRKLNTNNVMIVLVFILMLSFCICIIVKSDLFDVKKIDVIG
                     NKRVTKSNIMKELNVNLNENIFAYNFKDMKNKLIKNPYIENVEIKRKLPNKIIISLKE
                     KEIFAVLKDEDNYCYIDKKGNLLEELRGSNESKKDLIVDVDYSIDDNKSIKFKNYKTK
                     ENVFKTLNYLKEEGIYRKINYVNLKKESNIEMLTRSNIKILLSNDDNLDYNISRVSKI
                     LIDLQNKNTNGGTINLNYGKLAVYSPEG"
     gene            complement(43618..44844)
                     /gene="murG"
                     /locus_tag="PC414_000697"
     CDS             complement(43618..44844)
                     /gene="murG"
                     /locus_tag="PC414_000697"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDF2463109.1"
                     /translation="MKVLLSGGGTGGHVYPAIAIANKIRDEHPDAEIIFVGTEKGIES
                     EIVPKYGFELKTVTVQGFKRKIDFDNVKRVFKLFKGLEQSRKIVKKFKPDIVIGTGGY
                     VSGPVLFNASMGKIPAIIHEQNSFPGVTNKILSKTVTKVLTSFEDSHKRFPEAAEDKL
                     VFTGNPVRKEILLSRKNIARKNLSISDEKRMVLCYGGSGGSRKINDAMRLVIKNMVNE
                     DIAFIFATGKSYYDEFMGSISDINLKPYQKVVPYLEDMANALAASDLVIGSAGAISLA
                     EITALGKPSIIIPKAYTAENHQEYNAKSIEKQGAGIAILEKNLTPESLNTAVFKLLGD
                     RELLVDMANASKTIGKPEAIDLIYDEIMKVYNSTQKSTSKKTKKEKVIKEVKEIKKET
                     TPSIEGQAKVIGIKKR"
     gene            complement(44862..45992)
                     /gene="spoVE"
                     /locus_tag="PC414_000698"
     CDS             complement(44862..45992)
                     /gene="spoVE"
                     /locus_tag="PC414_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein E"
                     /protein_id="HDF2463110.1"
                     /translation="MPKENLKKQIDIRVKTEFDGVVFYTTMLLVFVGIVMVFSASFIQ
                     SSFKHNDAYYFLKKNVIYAVLGFIVMIITSRIDYSFWKKNATAIGAIAVVLLLLVLTP
                     LGIEANGAKRWLGIGALTFQPAEIAKFATIILTAKLIEKNYDKIKSLTKGVVPLLVVP
                     GIFFALIILQPNLSTAGTVILVTFVMIFVAGMDMKIVFAMIGSGAALFAALVIAEPYR
                     LSRVTSFLDPFQDPLGKGYQVIQGLYALGSGGLFGLGLGKSKQKYFYIPEPQNDFIFA
                     IIGEELGLIGCIIVIMLFVVLVYRCVRIALKTSNVFACMVVIGIGAQIGIQAALNIAV
                     ATSSMPATGVALPFISYGGTSLTIFMGAVGIVLNISKHVKIN"
     gene            complement(46044..47399)
                     /locus_tag="PC414_000699"
     CDS             complement(46044..47399)
                     /locus_tag="PC414_000699"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDF2463111.1"
                     /translation="MDLKGKKVLLVGLAKTGISTIKHLDKLGASIIVNDIKDENKLRN
                     ILDELKSINDIKYILGHHPEDVDDIDMVVVSPGVPLDLPFILKLKNSGKYIIGEVELA
                     FKLSDNPIFIGITGTNGKTTTTSLVGEIFSRAKRDTYVVGNIGNPVIDTIETSSEESV
                     LVTELSSFQLESIDEFRPKVSAILNITEDHLNRHHTMEKYIEAKANIFMNQTVEDFCV
                     LNYDDEIVKSLADKCNAKVIYFSRTKKVNGGVYLENNDIIIDIDDKIKFLNKDDVSLP
                     GGHNLENCMAAIAIAYVCKIDLEVIKDVLMTFKGVEHRQEFVRNLDNVIYVNDSKGTN
                     PDSTIKAIQSYDRPIILIAGGMDKGSNFDELLETAKSYVKSLVLLGETASNIENCAKN
                     KGFNDIHIVKDMEEAVKTSYEISKSGDIVLLSPACASWDMYESFEVRGKDFKDNVNNL
                     K"
     gene            complement(47418..48386)
                     /locus_tag="PC414_000700"
     CDS             complement(47418..48386)
                     /locus_tag="PC414_000700"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HDF2463112.1"
                     /translation="MMLGITELTYTALIAFLIVIIIGPIFIPMLRKFKFGQTVRDDGP
                     QTHLAKNGTPTMGGIIMIVAILITGLTRVKVSHDMAVGLICIAGFGFIGFLDDFIKIK
                     LKRSLGLKAYQKIILQVALSFYVAFYQYTSSSSASQLMIPFTDFVINVGILYIPIMMF
                     IIVAIVNAVNLTDGLDGLASGVTLIVSVFFMLFASSIAGNTEVAVLAAATVGACLGFL
                     GFNSYPARVFMGDTGSMALGGAVVAFSVLTNSVLIIPIIGGIYFAEALSVLIQVGYFK
                     ATRKRFFKMAPIHHHFEQCGWPETRVVFIFWIITVVLAWISIIAVF"
     gene            complement(48427..49800)
                     /locus_tag="PC414_000701"
     CDS             complement(48427..49800)
                     /locus_tag="PC414_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDF2463113.1"
                     /translation="MECLTIKELVLATNGKLIYGDYNDCVSDIVIDSREANAQNAFVA
                     IVGENLDGHTFMKSAYDSGCKTFIKNESNGIKLESSDINLIEVKDTSLALGDISKYYK
                     EKFDIPFIGVTGSVGKTTTRDMIYSAISAKLNILKNEKNLNNHFGVPLTLFNLNKEHE
                     CAVIEMGMSGFNEIKYLVDIVNPKIAVISNIGLSHVEKLGSQEGILKAKMEITSNFDE
                     TNTLIVNGDDKFLSTLKEKEHVYKLKTFGFNKNNDVYCESYTMEEDSLTFICVIDGKK
                     EEIFIPTIGEHNIYNAMSAILVGLCLNISLDYIKKGLKNFKGTKMRLDIIKNEKLTII
                     NDSYNASPDSMDAALKILGRYKGRRVAILGDILEMGEISEYGHRLVGKSSMNNTDIII
                     TIGENSVFIGEEAKQLGFNSANIYHFENREDVFNKLNELVKTGDTILVKGSRGMRLEK
                     IVEYLNK"
     gene            complement(49967..51946)
                     /locus_tag="PC414_000702"
     CDS             complement(49967..51946)
                     /locus_tag="PC414_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein D"
                     /protein_id="HDF2463114.1"
                     /translation="MRKVKRISKKRLVLVLILACALFFCLVIRTGYLQLMKGNWLSTK
                     ALEQQTRDIPIEPKRGTIYDRNMKELAVSVTKYTVWCKPVEVEDKKEAAEKVAEILDE
                     DYKDIYALISKKNMALVKVKRWIDDDKASQIRDAKLSGIWVAEDNQRYYPYGNFAPYV
                     LGHTSSDATGISGVEMQYDKKLKGKPGKLIVSTDASGREIPQGMEKYYEPVQGNGLVL
                     SIDEVIQHYTEKAVQKAYELNNAKKVTAIAMNPKTGDILALASKPDYDPNDSRTPIYP
                     YYQEELEKYNDKDKIKGYYQMWRNPAVSDTYEPGSTFKLITSSSALEEGVIKDGEKFT
                     CTGSVTVGGRKIKCWRHYRPHGTQEFKQAVQNSCNPVFVELGSRLGVGKMYDYIESFG
                     LMDKTGIDLPGEAKGILYNEKNVGPVELATISFGQSISVTPIQLITAISSIANGGDLM
                     QPRVVKSYTDNKGNITETVKPKKVRSVISKETSKKMLEIAESVVTEGGGKIAYIPGYR
                     LGGKTGTAQKVIDGKYAPGKYICSFVGIAPCDDPQIVVLAIVDEPTGVSAFGSTTAGP
                     IVKEIMNDSLKYLGVKPVYKEEEKAEYEKKQVKVPDVRNLKIGDAVKALEDAKLKPDL
                     DADIELPEDTKVKDIFPKPGVKVNEDSSITLYFEN"
     gene            complement(51958..52305)
                     /locus_tag="PC414_000703"
     CDS             complement(51958..52305)
                     /locus_tag="PC414_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463115.1"
                     /translation="MKKNYKIHNLNEYKNKKKNNYKKRNKKKIGAKCILLIATFSVII
                     LNLCGYSKISEMKYDIYYLQKDLRKKEIVLEELKAKETEKTSIKEIELKAKELLNMDY
                     PKDGQIKYIDVDN"
     gene            complement(52786..53721)
                     /gene="rsmH"
                     /locus_tag="PC414_000704"
     CDS             complement(52786..53721)
                     /gene="rsmH"
                     /locus_tag="PC414_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HDF2463116.1"
                     /translation="MEFHHVSVLLNECIENLNIKPDGVYVDCTMGGAGHSKEIVKKLS
                     DKGLFIGFDQDKNAISTAKERLSEYESRVKFVHSNFENIKEELEKIGVYKIDGVLADL
                     GVSSHQLDEADRGFSYMQDAPLDMRMDVRCEFSAYDVVNTYTEDELTKIIKDYGEDNW
                     AKRIAKFIVEERANKPIETTGELVDVIKKAIPKKARIDGPHPAKRTFQAIRIEVNNEL
                     GVITKMINDASSIMNEGGRICIITFHSLEDRIVKNAFKHLASDCICPQHLPICQCDKE
                     SEVKIITRKPILPSEEEIEVNPRSRSAKLRVAEKI"
     gene            complement(53751..54497)
                     /locus_tag="PC414_000705"
     CDS             complement(53751..54497)
                     /locus_tag="PC414_000705"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDF2463117.1"
                     /translation="MDRVAFTLFGIDIMWYGILMACGMILGTLIAIKEAKRVGIKEDD
                     VLNIAIIAIPVGLICARIYYVVFNWSYYAQNMSQIFNFRGGGLAIHGGLIGGILAGYI
                     YTKIKNINFLKMADTVILGMPLAQAIGRWGNFINGEAHGGATNLPWGIMVDGVKVHPT
                     FLYESIWDFGIFIVLLLFRKNKKYEGQVIVTYITLYSIGRFFIEGLRTDSLMLGPLRM
                     AQVISLIGVIGGIIAHVYLSKKNKNNISEE"
     gene            complement(54891..56177)
                     /locus_tag="PC414_000706"
     CDS             complement(54891..56177)
                     /locus_tag="PC414_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDF2463118.1"
                     /translation="MEKIVNDILKEEVYYEKLQNGLDVYFMPKRGFMKKYAILATNYG
                     SNDLEFVPIGEDKKIRVNEGIAHFLEHKMFEQPDGGDAFDKFSKLGVNANAFTNFTMT
                     AYLFSATENFYESLEHLIDYVQTPYFTDENVEKEKGIIAQEIKMYNDDPDWNVYFNCL
                     KAMYVNYPARIDIAGTVDSIYKITKEELYKCYNTFYNPGNMALFVVGDLDVEKVIDVT
                     KKSNNYKVDRLSKSIERFYPEEPESVKEKEVIEKFPISMPMFNIGFKDSNVGLKGKEL
                     LRKEIVTDILVGMLFKKGSKLYEDLYMQGLINENFGAGFSSQVDYAFSIIAGDSKEPK
                     KVKEIILDYIEKSKKEGLSKEEFERTKKKKIGSFIKCFDSINFIGNSFISYVFKDINL
                     LDYLDIIKDITFEEVEERLKEHFKEEYCVISIVEPK"
     gene            complement(56192..57457)
                     /locus_tag="PC414_000707"
     CDS             complement(56192..57457)
                     /locus_tag="PC414_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDF2463119.1"
                     /translation="MVNTKKIINLGNNINLTLIKTEKFKSNLISLYVQRLLDEKETTK
                     NALIPSIIASGSAKYPSARAISNKLDDLYGSSMGADAVKRGERQVLSFKVINISEKYL
                     DESIFEEVVEFFNEVINNTLVVDGGFKEEYLNIEKENLREKIQSIINDKKEYAQDKCI
                     EAMCKGERYSVSEFGYEDEIDSITSRELYEHYKNILKTSPIDIVVEGNFDEDKVVDII
                     SKNLKFEREEIINIPRADFIKNVDEVKVIDEQMEITQGKLVMGYRANVDYADIDKYYA
                     LVVGSNVLGGGPHSKLFVNVREKESLCYYIFSSIEKYKSIMFISSGIETKDYDKAVEL
                     IKKQVESVKAGDISDEELENSKLALVNSMKSITDNIGGMSDFVFSQSMAKTNSEVQDI
                     ISSIEKVTKKDIVEAIKNIELDTVYFLRN"
     gene            57773..58870
                     /gene="ychF"
                     /locus_tag="PC414_000708"
     CDS             57773..58870
                     /gene="ychF"
                     /locus_tag="PC414_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HDF2463120.1"
                     /translation="MKLGIVGLPNVGKSTLFNAITQAGAESANYPFCTIDPNVGVVSV
                     PDERLNKLQELYNSEKIVPTAIEFCDIAGLVRGASKGEGLGNKFLSHIREVDAIVHVV
                     RCFEDENVVHVDGSVDPLRDIETINLELIFSDIEILERRINKTQKAAKADKTLGSELD
                     LLKSIMSTLEESKCVRTMEFTEDEQTFVNSLDLLTSKPVIYASNVSEDDLADNGENNK
                     YVQQVKAFAETEDAEVVVVCAQIEAEISELDSAEEKKEFLETLGLEQSGLDKLIKSSY
                     ALLGLISFLTAGPKEVRAWTIKVGSKAPQAGGKIHSDIERGFIRAETIAFNDLVEHGT
                     MAAAKEKGLVKLEGKEYIVKDGDVILFRFNV"
BASE COUNT        18470 a        10669 c         6343 g        23428 t
ORIGIN      
        1 ttatttatta tttttcatta gtatataaaa agataaaata cataacttta tagacttaat
       61 ttattacttt tatagaattt tcacaataaa aagtcatttt acttccgtat ttaatgcaaa
      121 agtagaaatt aaacttaata taaaaaatat ataacaaatt ttttttatat ttctagaaga
      181 aaagtatact cttttttgtt taataatgct aaaattgatt tgtattgaat tttgattagg
      241 aggaaattat tatgaaagct caaagattaa atgctgtact tgaacaaatg aaaaaagatg
      301 atatttctca aatgttagta agtgacccta cttctatatt ttatcttaca ggagtactaa
      361 tccatccagg cgaaagacta ttagcattat acttaaattt aaatggcaat aataaattat
      421 ttataaatga attattccca gtttcagaag atttaggtgt tgaaatggtt tggtttaatg
      481 atactcaaaa tccagtagaa ataataactg aacatataga caaaaatgct actatgggtg
      541 ttgataaaaa ctggccagct agattcttat taaacttaat agaattaggt ggaggaagta
      601 agtttgtaaa ttcatcttat ataatagata ctttaagaat gtgtaaagat gaagaagaaa
      661 aagagttaat gagaatagct tcaaaattaa atgacaaggc tatggaacaa cttaaagcta
      721 ctgtttctgg tgaacttaca gaaaaacaac ttgttggtaa attatctaaa atatatgaag
      781 acctaggtac tgatggattt tcatttgacc ctattatagg ttttggacct aatggagcta
      841 atccacatgg tgaacctgga aatgcacttg tgaaaccagg agatgcaata atcttagata
      901 taggttgtat aaaagataac tattgtgctg atatgacaag aactgtattc tataaagaaa
      961 tacctgaaaa aggtagagaa atatttgaaa tagttcttga agctaataag agagctgaag
     1021 ctattgtaaa accaggtgtt agattctgtg atatagatgc tgctgctcgt gactacataa
     1081 ctgaaaaggg atatggacag tacttcactc atagaactgg tcactctata ggacttgaag
     1141 ttcatgataa aggagatgta tcttctataa atacagatac tgtacaacca ggaatgatat
     1201 tctctataga gccaggtata tatttaccag gtgagttcgg agttagaata gaagatttag
     1261 tacttgttac tgaagatggt tgtgagatat taaacaaaca tgacaaagaa atatgtgtag
     1321 ttggttaatt ctaaaatcaa tttgataaaa ttaaaaagcc atataaaaat gttttaaaca
     1381 tttttatatg gctttaattt ctcaatctat gatttatttc aatccattat ttatattgca
     1441 gttattaaga ttgcaccctt tgaagtatat gtccccatca ctgaaccaat ttcacagatt
     1501 acaacatctt tgtattttac atccttttcc ataacttccc ttaccttttg agccttttct
     1561 gggcaattag catgtcctat aaataaaacc ttttcttctt tatctttaat ttttgactct
     1621 actaaagtaa tcagcttttt caaactgttt ccttcacctc ttgccttatc tataggagtt
     1681 acaacgccat ctgcaacttg tattatcgcc ttaaaattta aggtatttat aatttttcct
     1741 gctattggat ttattctacc acccttaata gcattttcta aagtttcaag tgttccaaaa
     1801 aatggaggat tatctttata tttatttatt attgaaacaa tctcttcaat ttcttttcct
     1861 tcttttatta atattgctgc ttttaacact aattgtccta ttccaattga cccactaagt
     1921 gaatctataa cctcaacttt cttgttattt ccttcctttt caaacatatc ttttgctaaa
     1981 agtgcactat tataagtccc agataatcct gaacttatat taatcactaa tacactttct
     2041 tcctgacaat ggtatgcttc taaaaattta tcaggagaag gacatgatgt ttttggtagt
     2101 actttactct cactcatttt tctatagaaa gtttcattat ctatttcctt tccagatata
     2161 taactttctt ctccaaaaga tacatttaaa ccaacaactt ctatgttgta tttttctatt
     2221 atatcatcag gtaagtcaca tgcactgtcc actatcaatt ttattttatc catattaacc
     2281 tcactataag atattatttt atttattcag tcggtcgtac gaccgattta attattattt
     2341 tatatattga atgaaaaaaa gtcaatagat atagattctc gctttattga caataaatat
     2401 ccataagctt ataatgttat taatttgtta taaatataca tggaaaggta ggttttatga
     2461 aaacaaataa tatgaaacag gctattttat catctgcaac caatttaata gccaaaaatg
     2521 gagttcaaaa tactagcctt gctgatattg caaaagatgt aaatataagt aaaggtactt
     2581 tgtattatca ctacgcatct aaagatgata ttatatatga tattgcggat actcatttag
     2641 aagttataac tagtgctata ctaaactgtg taaaaaatgt caaatcaaaa aattctcaaa
     2701 tagaaatggt taacttaatc ttagagaaaa tatctactat agaaagtcgt ggtagggtac
     2761 atatgtatct tatttgtgag gcaattactt caaataatga tttaaaggaa agaataagat
     2821 taaaatatat tgaatggaga actactctta aatctgaaat tgttgaatca ctagaaaaat
     2881 ataatagtag cgaaaatgaa caggatgctg aatcattttc ttttttactc atgtcaatag
     2941 ttgatggttt agttgtccaa agtttattaa aaacagaaaa aataccttat gaaaatatag
     3001 cttctttttt agttaatcat tggatttaaa cttaatactt agataattaa taaaagtgaa
     3061 ctatttaagt tatctacttt aacagttcac tttcttattt attctcaata atttttactt
     3121 aaaacattca atttttattt aaaccttcat ggaatcacaa aataaaaatc tcttataaat
     3181 ttaaaatgtt tttatttatg ttcatgtgta tgcccatctg aatgttcatg accatgtgta
     3241 tgtgtatgct catggctatg ctcatgagta cctgaaaaac catcatcatg attatggtcg
     3301 tgacctacac catttacatg ctcatgttca tgcccatgac tgtgagaatg ttgatgtgaa
     3361 tgttcatggt tatctcctcc acagcatcct tctcctccag tcccacaaca cccacttgta
     3421 tgtggattat gatgatttct tactacaaat ctctctctta acataattct tatacctcct
     3481 agtatattat tgagtacttc atgtacccca ttaactacag tatacatcta actaggtata
     3541 atctcaatac atatggcatt ttttaacata tataattaac atatataatc ttaaagaaat
     3601 ttatccttaa aacctccacc ttttacttct tcaacgttat tgacagaaat aaaagcttca
     3661 ctatccatat cataaattaa atctttaaat ttacctattt catttgcaga tattatgcaa
     3721 taaatcatct ttttcttatt ttgtgtatat gcaccttctg cctccaaaaa agtgactcct
     3781 ctacccattt tattcattat cactttggat atttcatcat atttatttga tattaaaagt
     3841 atagattttt ttctatcaaa gcaatctttt cctatatcca tagtaaaaga aattatatac
     3901 attgtaatta aagtgtacat caccaattta gctccaaata aaaaagctcc tgcacatact
     3961 acaaaaaaat taataaataa aaatgtattt ttcataggta tatcatactt tcttttcatt
     4021 atggctgcta ttatatcaag tcctccagca gtagctcttg ctttaaaagt taaccccata
     4081 cctataccag ttaatgcacc accataaata cactgtagca tagtatcatc tattgggaaa
     4141 tatttagcaa tatcttgtgt tagccccagt gctagagaaa atagtaacat attgacaaaa
     4201 cttattagca aaaatttatt atcaaaatac tttctagcta taataaatat tggtatgttc
     4261 atcaagaaac ttgatattcc ctgattaata ccaaataatt tgtataatat aacacaaatt
     4321 ccagctactc ctccacttaa taacttggct ggtgctagat ataaatttat tcctaaggtt
     4381 gatagaatca tacccaatat aataagacaa tattgtaaaa atatattatt atcaatactt
     4441 agctttttac tttcaccata catcaaaacg cacccctttg cataataaat aaaccaccct
     4501 gcataacaca cgataaattt acaaataaat ttatcagaag tagtttccta aaaatattat
     4561 tcatcctcac ttaaacgttt attcattaat attctatcac atgacataaa taatttatat
     4621 gatttatata gatttattta ttgtaaattt tggaatatta aaacatttta ttgaatagat
     4681 aattatatta cataaaaaat ccctatagaa atctataaac taatttctat agggattttg
     4741 gttaactaac tattattttt atttttttat aacttttata cctgtcatat ggtcatttaa
     4801 gttttgttcc tctaaagatt tatcatctac tctgtttatt tctaaagcta aagtttcaga
     4861 cttgatatat tcattaaaat ggtcaacagc cttagcaatt tcatcatcac cacagtattc
     4921 tattactata ttatctaata cttcaaagtt attagatttt cttaattgct gtactttaga
     4981 tataaattct ctagcataac cttcattaat taactcttca gttagcttag tatctagtat
     5041 aacaaaaaga ttgttttcaa cagatacatt aaatccttcc ttagcagtta tacctattaa
     5101 tactaaatcc ttattaaatt caaaagcttc accatctaaa tcaactgtta aagtctcacc
     5161 tttttcaagt ttattagcag tttcatgagc atttaattta cttaatgctc caacgaataa
     5221 attcatctta gaacctaata atggccctgc ttccttaaag tttggtttta aactaaagtt
     5281 catatactca tctaaatcct tagcaaatat tacctcttta acattaagct cttcttttat
     5341 taaatcaact acatcactta ttgtatcttc aaacttacca tctactaaca ctttttgtaa
     5401 tggttgacga acttttattc tttctacttc tcttgaagct ctacctaaag taactaagtt
     5461 tttagctaaa tccatttttt cttctaattt agtatctact aactcttcat tacatttagg
     5521 atattcagct aaatgaacag atacttctcc tgttaagttt ctatacattt cttctgacat
     5581 atatggtgca aatggagcta tagctctaca aagaccatgt aacaattcat aagttgtatt
     5641 gtacactgct tttttatctt ctgttaattc agttgcccag aatcttcttc ttgaacgtct
     5701 tatataccag ttagataaat catcatttat aaaatcttgt atcattctta aagttttatt
     5761 taactcaaat atagctaaat tttcttctac atctttcatt aaattattga acttagataa
     5821 tatccatcta tcaagttctg gtctatcttt atattctaca aaaaattcag ttggatttat
     5881 atcatctgtg tttgcatata aagtaaagaa gttatataca ttcttcatag tacctataaa
     5941 tttactttgg atttctttta atccatccat atcaaattta gttggagtcc atggtggaga
     6001 aacatatagt aaataccatc ttaatgcatc tgctccatat tggtcaaata attccattgg
     6061 atttactgta tttcctcttg atttactcat tttcttacca tctttatcaa gtacaagatc
     6121 tggaactaaa acactcttat atggtgcttt tcctaataca aatgttgata ctgcaagtaa
     6181 tgagtagaac caacctctag tctggtctat accttctgat atatagtcag ctgggaacaa
     6241 ttggtcaaag ttttccttat tttcaaatgg atagtggtgt tgagcaaatg gcatagcccc
     6301 actatcaaac caacagtcta taacctctgt tactcttgtc attggttttc cacatttttc
     6361 acattttaag tgtatatcat ctacatatgg tctatgaagt tcaactgttt ctggatttac
     6421 atcttctata gctttttcag ctaattcagc tcttgaacca actgaatctg tatgaccaca
     6481 ttcacatctc catataggaa gtggagttcc ccaatatcta gttcttgata ttgcccagtc
     6541 atttagattt tctaaccagt ttccaaatct cttctctcct acaaaacttg ggaaccattt
     6601 tacaccatta ttattttcta ttaatttatc tttaagttta gtcatctcta tataccagct
     6661 tggttttgca tagtatacta atggagtttg acatctccag cagtgtggat aattatgaac
     6721 tactttttct ttgctatata atttattttc agaagctagc cattttatta tttcaacatc
     6781 tagcccatct tccattacaa atctaccttc ccaaggagta gatgtaaatt taccagtttc
     6841 atctactggt tgaataactg gtaaatcata tcttttacct gtgttgtagt catcttcacc
     6901 aaatgctggt gctgtatgaa ctataccagt tccatcttca gtagttacat aatctcctac
     6961 tgtaataaaa aatgcttttt tgtcaacttc tacaaaaggc attaattgtt catattctaa
     7021 accttctagg tctttacctt tcatttcttc taaaacttca taatcttcac ctagtacttt
     7081 atcagctaaa ggtttagata cataatatac ctcatcacct tttttaactt ttaaataatc
     7141 tacatcagca tttactgtta atgaaacgtt tgatggtaaa gtccatggag ttgttgtcca
     7201 tgctaagaag tattcatcag catctttttt cttaaattta gctataactg tattagtttt
     7261 tacttcttta tatccttgtg caacctcatg actagcaaga ccagttccac atcttgggca
     7321 ataaggaagt attttatgtc cttcatatat atatccttct ttgttgaatt tgtttagtat
     7381 ccaccataca gactctatat agttattatc taaagttata tatggattat ctaaatcaac
     7441 ttcataagcc attctctcag tcattattct ccattgtttt tcaaatgaga aaactgactc
     7501 tctacatttt tggttaaatt cagctattcc atattcttct atttgttgct tgctagtaag
     7561 acctaactct ttctctactt gtatttcaac tggtaatcca tgtgtatccc aaccagcttt
     7621 tctctttact tgataaccag acattgtttt atatctacaa actgaatctt taagcgttct
     7681 agataaaaca tggtgtactc caggatttcc attagctgtt ggaggtcctt catagaatac
     7741 gaaacttggg tcatctttcc ctttttctaa agttttttca agtatattta tgtctttcca
     7801 ataatcaaaa acttgtgctt ctgcttgttt tacagaactg tctaccaatg gcttaaattt
     7861 cgccatgtaa atcacccttt cctactaaat attataattc caaaattttt tattctttca
     7921 cataaattta aatactaaaa aactcgcccc ctttaaacca aagggacgag ttataaatcg
     7981 cgttaccacc ctaattctat atgtaaacta aatactagct acatataaca ctctaatagt
     8041 ataacggtac taaccggcac agcttagtag aatttaatcg ttcagcctgc agctcaagag
     8101 tgatttttat ctttataaat tcattggttc tcagcatacc caattctctg taaaattatc
     8161 taaagataaa tgtctctatc aacgctttta aaaatttatc tgtgtttata tgaaattcta
     8221 taacaaataa tttgaatagt caatatattt ttgaaaaata taataatttc tatttttctt
     8281 acatttttat cctattttta atctttgtta tagatttctc aaactcacac aattattcta
     8341 cataaaaaat aaacaaatta caagcttatt ctaaagttgt agcagcttca tcattactat
     8401 aattatacac tgttgttcct tcaaatgcat ttacacattt ttcatctaca tcataaaaaa
     8461 tctcatcaac acttcttatt tcatcttcta acagtgattt aaatttattt ctaaatatct
     8521 taaattcttt gactaaagca tcatactctt ttcttatctc tataacacgg ttattagctt
     8581 gttctataat ttgtctagct tttaaatcag cttcttcaac aattatctta gcctttttat
     8641 tagccgcgct acaagtgtct tctgctgcac tttgagcagt tattaaagta gcttttagtg
     8701 tttcttcaat attttcatac ttgtttactt ggtcttgata taaccctaat ttttctttaa
     8761 gagctgtatt ttccctacat agagattcat aatcttcctt gatgatatct aaaaattcat
     8821 caacctcttc atctctatac cctcttaaac cttttttaaa ctctttattt tctatctcaa
     8881 ttggagttag cattacattt cctcccagct ataatttaaa ttagtcctta tacaattaat
     8941 cttgcttgaa cttttatttt tcctttttta gtaatatctc ctatttgtgt taccttagct
     9001 cttcctttgc ctctaataga aatcaaatca tcgtttttta ctattttaga agttgaagtt
     9061 atcttttcat aattgacatg gactttttct ccatttatgt attttgcact atcttgtcta
     9121 gatatattgt aaataccact tatcacacaa tctaacctat ctgaagatac tgtaaatgat
     9181 acttctttat attttgaaga actattaaca atattttctc tggaaatttc ctttacaatt
     9241 acattatttc ttgaaacctt ttctaagttt acaattataa aatcgcaaat atcactactt
     9301 acaactactt gacaaaagct atcatgtata attatatctc caattttttc tctttttata
     9361 cctagaccca gtattgaccc taagtaatct ttatgagata tatttttaaa tttgaaattc
     9421 ccttctattt gtaaaaatct aagagtatct tctatatctt catattccat gtaaaatgga
     9481 taaataaata cagtgcttct ttcagcttgt tcataacctc catccacact gtattttatt
     9541 tcatttgtag aatttaatat tgctacagca tttttcactt catatggatt aagaaactcc
     9601 gtagacttaa catcatagtt ttttatacaa ctattagcct tgtctatcac tttaaacatt
     9661 ttatttttca agtctatatc ctttatgtga tttgttagtt ttaatttatc catcctatca
     9721 ccttagaata taaccaatac taattgtctt aggaacatca ataatactat tgcaattaaa
     9781 ggagaaaaat ctataggtcc atttgtatat ttatacatta tagaccttat tggcccttct
     9841 attggctctg taagattttc caaaatatca aaaattttac tttgtcttcc atttggaaac
     9901 caagtcatca agctttttat aactatcatc catgctatta tatctaaaag ataataaagt
     9961 gcaattctta ttgttcccat atattaaccg cctttttact atttattttg ccaagggaag
    10021 aatgctttac tttctaattc ttttttcata tttgcatcta tatccacatt attaggagct
    10081 agtataaata ttgatttaga aaccttttgt atacttccat ctaagacata aactgcccca
    10141 ttcataaagt taaatataga tttacgaact tcagaattag tcaatccttc caaattcact
    10201 ataactgccc ttctttgttt tagatggtct gctatagttg cagcttcatc ataaacctta
    10261 ggttcaacta ttacaacttt catttggcta gttgtatgta aatttacaat tttatttgct
    10321 tttgcagtac taaagccact gttcatttct tcttcttgaa ctatatcgtc catgccactt
    10381 tcatattcat cttcaatgta atcttcttct tcatcaacta tccaattctt aaatttagat
    10441 attattccat ttgacattat taacttcctc cttaaaattt attcaaattt gagtaattat
    10501 tttttattgt agtttctttc tccaaagatt gatgttccaa ctctaacaat ggtagcccct
    10561 tcctctatag caatttcata atcgtgactc attcccattg acaaaacatc catccctaca
    10621 ttagggatgt ttagttcttt tatttgtttt gataaatctt taagaccttt aaatacacct
    10681 cttatctcat cttcatcttc tataaaaggt gccattgtca ttagaccctt tatttttata
    10741 tttttatatt tttctgacac ttctttgaca aagtctacaa cttcttctct atcaagtcca
    10801 tgcttagact cttctttaga tatattcact tgtactaagc aatctatttc tctgtttatt
    10861 tgttctgcac gtttttgaat ttcttcgcat aatgcaattc tatctaaaga gtgtatcata
    10921 caaactttat cgattatgta cttaacttta ttggtttgca aagttcctat caggtgccag
    10981 tttaatttat cacctataat atcatacttt ctagctaatt cttggggttt attttcacca
    11041 acagaagtga ttccttccct cattgcctca ttaactaagt ctacatcaac agtctttgtg
    11101 actgccaaaa gggtaatctc tttgctgtct ctatctactt tttttgcagc tttatcaatt
    11161 ttgtttctta tctcagacaa attatcttta atagaaccca ttattatcac ctactttatc
    11221 cttatatttg tgtttatact gttaggtctt aaaattattt tgtcatatag attaacactt
    11281 gtaactccat ttatgtcatt tttataataa tccacaaata cagaagattc attctcataa
    11341 gttattcctt ctaatatgac aaattttaca ctttgtgatt gttcatcaat tacatataca
    11401 ccacgtttat tgtcttttgt ttttatagaa gttttaggta tttttaaaca ttccatctga
    11461 ttatatatta tatcaaattc ttcgacactt gtatcctgat ttgcaacaat ttggttactt
    11521 atttttaatt ttaccattat tttattatta ttttcatata ttttgtaaac ttttgcatct
    11581 agtttttgct catgatattc aattattaca ttttgatttt tttcaaagtt attagaatct
    11641 tctttactga tgtatgtaga tagataatta ctttggtcat tggcatcttt ttctatagtt
    11701 ccttttacat gaatctttgc atcaactttt tcatttttta acaccaacgt atctacattt
    11761 ttgctaatta tcattactaa tatattaaat actaaatata taagaatacc aagtatcact
    11821 aatctggtat attttacctt tttcaaagac tctcccccta taaaattact ttcattataa
    11881 gtacttcttc taaaaaatgt ttattccttt tttataagat aatattttac gttctaataa
    11941 ttattttttc catattaaat ttatttttta tcaatcttcc tatacaagac tgcattattt
    12001 atatataatt gtattataaa gtataagttt aatttacatt ttatacgtta agcttacttt
    12061 atatattttt atagttattt ttatttttag atgcaatata aaaccaatta agatttaaaa
    12121 taacttttgt tatgttgaca tttatttagg taatcattta tgaattagga taacgttatc
    12181 acaacaaaag tttatgtcct aaaaaaaata caaggaggat tttcttatgt cagaaatttt
    12241 attaggaaca ggtttacttt tattggttct atgttgtttc acaattttta gttacaaggc
    12301 cccatatggt atgaaggcta tgggagctct agcaaacgct gcatgtgcta ctttcttagt
    12361 agaggcattc aacctttctt tacttggtga tgttttaggt atggaatttt ttagaagtgt
    12421 tggagctgct aatggtagtc ttggtggagt tgctgctgct atacttgtgc ctttagcctt
    12481 aggtgtatct ccagtttatg cagttttaat aggattatct tgtttagatt tttctatatt
    12541 atctggattc gttgctggat actttgtttc tttcttaatt aagtttatag agaaaaaagt
    12601 gccacctgga gtggatttag tggttgtaat tcttctatgt gcaccactat ctcgtcttat
    12661 agcatcaaat gtagacccat tagttaatag tacactacta caaattgggc aagttttgat
    12721 agaagctacc aacattagcc ctataataat gggaattata ctaggaggtc taataacagt
    12781 agttggaacg tctccactta gttcaatggc tcttacatct ataattggtc ttactggtct
    12841 tcctatggca ataggagcat taggtgcttt tggttcagct tttatgaact caacactatt
    12901 tgcaaaattg aaattaggtt ctaaaaaaga tattatatct gttgctattg aaccattaac
    12961 ccaatcagac ataatttcag ctaatccaat acctatatat tctactaatt tctttggagg
    13021 agccttttct ggtatcattg tagccttgat gggtcttgta aacatgacac caggtacagc
    13081 tacacctgtg gctggttttg ctataatgtt tgcttataat ccagttcaac aagttcttat
    13141 aacagctggt ttatgtgctt tattcaatgt aattgcagga tttttaggtt caaaaatatt
    13201 cagtaccttt aaagttctta ctgctggtga aatacgtggt actgatgtag aagaagaaat
    13261 tgcttaaaaa acaataagat ttataaattt atatctcaag tagtagagtt ttatattgta
    13321 ggcaatttta ttaattgcaa acgttttatt ttttatttat gtttatgatt attatgtgtg
    13381 ttgaatattt agataaattt ttatttatta tttttattta gatttttgtt tagtttttta
    13441 tttggttgat atattaggaa taaaaattag tatagtttat tactagttag atggcataac
    13501 tttttagata cataaaaaat tttatattaa ttagatatta ttaaaataaa agcgatacat
    13561 gaatttttaa atgtccatgt atcgctttat ttttgccata atcttaataa ctgattttta
    13621 aagtttatta tcttaacaaa gttcattatc ttatataata tttaaatgca taggcttctt
    13681 taatttaacc atctaaatat ttaattaaat attattatat gtaatataac taatttttaa
    13741 atgaatgtat aggagctgga atccttcctc ctctttgagt aaataattca gatgaatttg
    13801 tatttatagg cattactggt gctcgtccta aaagtcctcc aaattctacc atatcaccta
    13861 catcacatcc tggggctggt ataattctaa cggctgtagt cttattatta atcataccta
    13921 ttgctgcctc atcagctatc atggctccaa gcgtacctgc tgtagtattt cctggcacag
    13981 ctatcatatc aagacctaca gaacaaatag cagtcattgc ttcaagctta tctatactta
    14041 aagcaccttt aataacagca tcaatcatac ctgcatcttc agatactggt ataaacgctc
    14101 cactaagtcc tccaacatgt gaacatgcca ttactccacc ttttttaaca gcatcattta
    14161 aaagtgctaa tgctgctgta gtcccatgag taccaacaac ttcaagaccc atttcctcta
    14221 atatatttgc tactgaatca ccaatagcag gtgtaggagc tagtgataag tctattatcc
    14281 caaatggaac atccaatcta ctagaagctt cctttgccac aagttgtcca actcttgtaa
    14341 ttttaaatgc agtcttttta actgtttctg aaacaacatc aaaaggctct ccctttactt
    14401 tctctaaagc tctcttaaca acaccagggc cacttacacc aacatttata attctatcag
    14461 cctcaccaac accatggaaa gctcctgcca taaatggatt atcttctact gcattacaaa
    14521 atactactaa ttttgcacaa ccaaaacctt ttgcatcttt tgttaattct gcagtttctt
    14581 ttatgatttc tcccatttct ttcactgcat ccatatttat accacatctt gtagaaccta
    14641 catttactga agcacatact ttattagtac ttgctagagc ttttggaatt gacttaatta
    14701 gtatcttatc accttttgta tatccctttt gaactaatgc tgagaatcca ccaataaaat
    14761 ctatacctaa atcctctgca gccttatcca atgtctgtgc aaactttaca tagtcatttt
    14821 catctgttgc acctgcaata atggaaattg gagttactga taccctttta tttacaattg
    14881 ggataccaaa ttcacgctct atatcttttc caaccttcac caaatcttta gcagaactca
    14941 taatcttatt gtatattttt tctctagctt tatctccatc agggtctata caatctaata
    15001 aagaaatccc catagttata gttcttatat ccaactgttc ttcatctatc atctttattg
    15061 tttcaagtat atttcctaca ttcactatta tcacctcccc ttactgtcta ctacagatta
    15121 tgcattgaat taaatatttc ttctcgctga atttttacat ccactgataa tttcttacct
    15181 ctttcaacca agtcctcaac cgttttctca aaagaattta atgatttctc taaatcaact
    15241 atcataatca tagtaaaata atcctgtaat attttttgtg atatatctac tatgttgata
    15301 ttttgcttgt ataattcatc actaattcct gccactatac ctggtttatc ctttccaatt
    15361 actgttaaaa ttgctttcat aataatatcc tcccttattt ttttataaat cttatatttt
    15421 tacaaattga actatgtttt aacatatatt tcatttattt gttagtaaac cactttttat
    15481 gtatatattt aatatatttt aatacctata cattcatttg tcaatttttt ttcaatattc
    15541 tattattttt atactattta gaaaattcat atattatata tgttttctca acaataaata
    15601 cagttttcat aataatgaaa ggtattactt agcaatcgca tgattttcta aataatacct
    15661 ttctaaaaat tattttttta attttatgaa tttaattata ctttttctag tatcaggatt
    15721 tacaattaat atagaaccaa tcataattgg tgttagtata caaaataaaa aaataatatc
    15781 tttttttaga ttatattctt tagattctaa tttataatta ttatttatta ttccatcata
    15841 atcacttata tttgacttat ttttatttgt atcttgattt ttgttagaaa tactttccct
    15901 ataaggaaaa gaatgttcat tggtaccaaa tactaatttt gagcttaaat cataattatc
    15961 atcattttct gtaattgaat ttttattgta aaatcctttt tctttatcag aattattttc
    16021 tttattaccg tctttaggta tatcatgagg atttttattt tcattgctat cagtacttga
    16081 ttctacatca tctttaggag tatttggttg tttctcatca gaattatcat tttcattatt
    16141 attattttca ttattattgt tttgattgtt attactatca ttattttcat ttccattatt
    16201 cccatcttga ttgttatcat cctcatctct atccgggtcg tctctacctc ctcctccacg
    16261 gtcaccatca atatcatcat tttcccgttc atcatctggt gtctcttggt tgtctccgcc
    16321 tcccattata aatcctggtt taaaaactat atttgaatac ccttctttta atcctcctac
    16381 cacttttact ctaaaacaac gcaacttaaa atcttcaaaa aaaagtatag cttgatctct
    16441 aatatcctcc tgttcttcag atatccaatt gattccatcg cttgaatatt caacaaaaac
    16501 cttacttgca ctatacggca tatcataaaa taaaacagct acatatccat tttttgtatt
    16561 ttgaaattcc agttccaaat tgtctattgg ttttttctct ataacagata cattcaaaat
    16621 aggaactaca tcatcattta ttataatttt ttctttattt aacatttctg ttttaaactt
    16681 tcctgttata ttaaaatttt ctcccttttc caacttttca taataatcag taaaatccca
    16741 ttctacatct atatcaagtc gattaccact agatgtttct aataccatat atttgggaaa
    16801 ttttatattt tctattttat catctttact tacttttatt tcatcactaa atacaccaat
    16861 tcccctatga caacatttca caatatcata gccagaatct tcagctatag agggtataag
    16921 ctcattataa tcatcaatta tataaacttt cttactttct gatataattg attgtgccaa
    16981 cttattatta tcttttagta acccactttc attgttgcta ctatatgctt ttattgaagt
    17041 ttcttctatc tcaacatcac ctttagaata aacacctata gcatgttctc cattcacttc
    17101 aatatcctta tttagtattc taatatcatt ttcagtataa attcctattg catctttccc
    17161 atttgccttt attttttcaa cagattcgta ggatgattta atatgtaagt ctcctccttc
    17221 ttcaacgtat aaggctgtcc catcagtagc aattatgttg tttataccaa tacttatact
    17281 tccaccattt tctatatgta ttactccttc ttttcctcct tcacctatta tattcaattc
    17341 attatcatca ataataaatc tcccatgatt ttttatcaat attttatatt tgttggtatc
    17401 tattgtaaaa ttactgtcat aaggaatata aaatctaaac tcttcatcta ttaccatatc
    17461 acaagaaaga gtataaacta tttcttcttt attattttta ttttcttgaa cccacctttc
    17521 aaaatcttca agacaatttt cctcttgtcc aaaaacttta tgtggaaaac ttataaaaca
    17581 caaaactatt atactcataa aaataaatat ctcatacttt ttcatcttac actcctagaa
    17641 attttttagc tttgaaacaa tctgtcgaaa catacacttc acccactata atttttccac
    17701 aattatcaat tattgtcaat ttataaaagt atctttatta attgttataa ataaaaagct
    17761 acttactaaa gtaaacagac gtttcacttt aataagtagc tatattttta taacaaaata
    17821 gttataatat tagaacttcc ttcatttaca attttttgta aagtcttttg cattttaact
    17881 cttatctctt ctggcatagt atataattta ctttgtaatt gctcttttac taagtcatgt
    17941 agtgatttac caaacatatt tgattcccat aaatctgctg gttgctcttc aaatacttcc
    18001 ataagatatt ttatcatctc ttcaccttga ttttgatttc caactattgg agaaacctct
    18061 gtagatatat ctgcttttat aatatgaagt gatggtgcat tggcttttaa cttgatacca
    18121 tattgttttc cttgtttcat tatttctggt tcttccagac taagttcttc taaggaagga
    18181 gctactactc catatccttt tatcttggca tctattaaag cactttctat tttatcatac
    18241 tcatttttaa ctttagatag tctagttatc agactaagca gttgataatc tccttcaatt
    18301 ttaaatccac ttttttcttc aaggacatta tagaataaat cttgctttgt ctgtaaatca
    18361 atatttataa caccttctcc tagctctaca ttatcaacac ctgtatcttc caaaaactct
    18421 aactcagaaa atccttgtat aataccttct atatccctta ttttacctat atctataata
    18481 ctttgtttta atgttgttat tatgctactt tttatccaat gatttctttc aagaccttca
    18541 acccattttg gtaagttgat tcttatttct gtaagtggaa aatcatataa tacagcttcc
    18601 ataacctcct ctatatcttc ttcttccatt tcaaccacat tcattggaag aactggaact
    18661 tcatactttt cttccaattc acttctaagc atactcgttt cctcactttt tggacttaac
    18721 gtatttaaaa ccactgcaaa tggtttttta agatttttca attcttgtat aactctctct
    18781 tctggttcta catagctttt tctgtcaatt ccagtaactg aaccatctgt caagacaact
    18841 atccctattg tagaatggtc ttttataact tttttagttc ctatctctgc tgccttttca
    18901 aatgtcatag cttcttgtga ccaaggagtg gatactaatc tttgttttcc accttcctca
    18961 tgccctaaag ctccttcaac tatgtatcca acacaatcta ccattcttac ttttaaagat
    19021 actgtgtctt ttatttttat ttctacacca tctgctggta caaattttgg ttctactgtc
    19081 attatcgttt tacctgaacc actttggggt atctcatctc ttgttctatc ctttttaaat
    19141 tcattgtcta tattaggtat aaccaacttt tccataaatt ttcttataaa agttgatttt
    19201 cctgttctta caggtccaac tactccaata tatatatccc cctgagtcct tttggatata
    19261 tcttcgtata tgttattatt catattattc ctcctataca tctaatctta ttaaattata
    19321 tttagagtta agggttaata ttcttaaaat agaaaaataa agaccagcaa tattaattta
    19381 atttatatat agaaatttgt aaatttcaat gtaaattaaa ttaatattgc tggtccaaat
    19441 agtagtgtat tatattaact gactttagta gtattttcat ttgaattatt attcatctca
    19501 ataagttctt tacatctttc ttttgtcatg ttcatccata taaatgtaaa caccaatcca
    19561 attatggcta ctatagtaag aactatgaag tagtacctat atccagttac acccggaaat
    19621 gcatccaata tcttccctgc tgctaaagga caaagtactt ctggaagata tcctaacgta
    19681 gcaactattc ctatagcagt tcctgatatt gataatgaat aattaccttc ttcaagtaac
    19741 gaatagtgta aaccttgaat tgcatacata gatacatata ttgcaacaca tccaatcaat
    19801 agtatataaa tcatagatga ttttgttggt gtaaatatta cacctaaaag acctgcaatc
    19861 ataagtataa atccataaga tataatcttt gatgaaccaa ctttatctcc aagtaatcca
    19921 gaagcagctg atgctactgg tctacaatac tgagctaata ttgagattgc agatgcaaac
    19981 accacagtag ctccaaatgc ttcagttgaa taaggcgtaa aataataaaa tgacatatta
    20041 gctgaatatg aacaaaacat tattattgtt attatccatg tatgaggcat ttttattact
    20101 gcaaaaaagg cttctttatc aaattttgct gatacctctt tttcattatc ctttaatttg
    20161 aaaaatacta atactccaac tattacattt attgctgagt ataatactat aatagctgta
    20221 agaccagatt catcacttac ttttgatgaa atatacccaa atagtatcaa tactagagat
    20281 agatgaattg cattggtaat tccacgacca ctttccataa atccaaaagc ttttccttgt
    20341 tcattttcag atgctaacat tctaacagct tttacaagag ctggccaaaa agtaagtatt
    20401 gttgttattc cccataaggc atggattaaa aacacaacaa tatatggagg ataaagtgtt
    20461 aatacaagac cagatattcc tgtaataatt aaagaacctg aaagcaattt tctggctgaa
    20521 atacggtctg ctaaaaaacc accaaaaagg tatgatacca ttgcaagtag accataaatg
    20581 cttcctaagc ttcccatttg tgtatttgta agattaaatg cttgtgcaaa agtatcataa
    20641 taataattct ttaaatatgg taaagcatat ataaatgctc ctgctacaaa gagtattatc
    20701 aagactttga aatttttcca aatgcttggt ttatttactt caactgacat acttcctcct
    20761 tatgtaaata cctgtaaatt ttcataataa ttaattattg cttattttat tgtttattca
    20821 atatttagga atatttatta atatattagg ttaatctatc aataatttat ggtgagttat
    20881 gagctaaaag taaactcttt tagctcacca taataattat atttttattt atactaacat
    20941 ccagagttat tagatatctt tttttcatag ttcttccata gcattaaacc tgttattata
    21001 acaaaaagac ctcctcctat catttcaatc tcatagatta tattatcttt taaagtctcc
    21061 ataactggtg ccgttgagaa aagtatgcca actacagtta ctacaagtac cattctggca
    21121 atattgatac aaactgaatc actttttgca attgaatatg gtctttcttc atttggtctt
    21181 gtctttctaa gtctaatata agatgtaaaa agcaagacat atggaaataa tgctgttaat
    21241 gctgtcatag taacaagaac attgtatata ttatctactg atggtaataa gtttgttcct
    21301 acaagaagta gactcactat tatagcctga actattactg catttgatgg tatattgtat
    21361 ttatttgtaa cagtcaattg cttaggaaat acaccttctc ttacatttcc aaaaagcatc
    21421 tttactggac ctgctatata aagtattaat gctcctagta ctgataaagt tattccaaat
    21481 gctactattc ttataaacca acttcctata cctaaatctt gtgcaacttt agctaatgca
    21541 tctagtatcc ctgtagatgc tgcaatttta tctggaggaa gtatcattgt tattgctact
    21601 gaacctaaga tatagattcc tcctattaaa acagccacag ttaatatagc tttaggaaag
    21661 tttttcttag cattatccat ttcggttatg aaattagcag tcgtttcagc tcctgataat
    21721 gcaaatatta tagcagatat agaaactaat gaattggcat ctatttttgg tattatattt
    21781 tggattgtat atatcgatgc tgatggagct tttttgagaa taactacaga cataaatgcc
    21841 atcactataa gtaatatagc aggaattgta gaacctaatg ctcctgtatt cgtaaaaaat
    21901 tttccaaatg ccatcccacg agtacttact aaggacaaca gccaaaatat tatgagtgaa
    21961 agtgctaaaa taaacatctt attgtttgat aaatctggat ttccaagcat atatgcaata
    22021 tttatcgcta aaaaagtcaa gaaagaagaa taccaaaata tctttgatgt ccaatttagc
    22081 catgacacca taaatcccca cttctctcca taagcttgtt taacccattc tccaagtcca
    22141 ccatcttttg gatatgttgc agccaactct gcacatataa gtgatgcagg aacaaagaat
    22201 ataaaagcaa acaataacca aactggtata gaaccaagac ccaatccaaa actagaggca
    22261 gtagatttag ctatccatct aatcccaaat agtgctgata tattcataaa tactaaatcc
    22321 caaaatccaa ttttcttttt cactgtaatt cctccaattt caaatttatt cagactcata
    22381 cacaatgtat tgacttaaat atcaagtata agttcttcaa cacttcttgg ataattagtt
    22441 ataattgtag caccattctt acctactatt gctgtatctg agtgtctaaa tcctccaact
    22501 ccaggtatat atatccctgg ctccatagaa aataccattc cttcttctaa tattaattca
    22561 ttgtcaaatc ttaaatatgg ctcttcatgt tctgataaac ctaaaccatg accaattcta
    22621 tgatttgaat ataactctag accatacttt gcaacaacag ctcttgctgc ttcatccacc
    22681 attcttgctg gtataccagc ttttattgta tctaatccag cttgttgagc ctctactgca
    22741 atcttaaaca cttctttttg tctttctgtt ggtttgccta taataaaagt tctttcattt
    22801 tctgctctat agttttcata ccaaacctgt ctactatgta ttactatatc tcctctttga
    22861 agtattctag tattggaatc taaatgaggt tgtgctgttc tatcaatccc tgaacaagtc
    22921 cagtttgcaa atcctatata tgtatcagga taattttcag aaacatattt tagtaaagca
    22981 ttatcccctg caacgtcaaa ctccaattca ctcatcccaa ctctaacatt ttctaaagac
    23041 ccttttattc ctatatcaga taggtaacct gctatcttca aaaattctat ttcttttgca
    23101 tcttttacat atctcatttc aaatatttta cttccaacat ctttgatagc aaatgactta
    23161 tcagttatat actctgaaaa gcttgcagat actatatctt tttctattcc aacagttgtt
    23221 cctttagggt atttatttaa aattatatct aagtaatgtt tatgtgatat tccatgttct
    23281 ttcatttctg gtatttcata atacacatat acattatcaa cttttgcaac ttctttagca
    23341 tgtaattctt ctaatccagg tactattaat tctattttat cttgagttac tactgttaca
    23401 attggtctag aataagttat tgctttaaat ccactaatat aaaattgatt gtctggttca
    23461 aatattaaag caatttcaat gcttttttca ttcatgtact ttctcaatct ttctagtctc
    23521 atattatttg acataatgca cctctttctt aatattcaga taatttattc aatacaacaa
    23581 ggatgaatat attattttaa aattcttaat atttttagta cttgtcgtat ttgtatgtaa
    23641 attaatttta tttgtgatta ttttatcata atcatgatta ttttgtcaat catgatttta
    23701 ttttaaaaaa ttttttatat tctagtgatt ttaaattatc taaaatataa cgagtataca
    23761 ttgtattttg taatattatt cttgatttat ggtaagttta atgataaaat cacgattata
    23821 taaactatat tgattatttg taggtgaata aaatgacaaa taaagaatta caaaagaaaa
    23881 ggatattgat gtatttcata gaagctgctc aaaatattat ggaaaacgaa ggtatagaaa
    23941 atattacact tcgtaaggtt gcagatatgg ctggctataa tagttcaact ctatataaat
    24001 actttaaaaa tttagaccat ttaatatcat ttgcatctat aaaatatttt aaagattata
    24061 acttgaatat ttcaagatgt atagaaaatg tcaatgatga atataagaaa tatataatta
    24121 tgtggaaatt attttgtaag cattcttttg ataatgctca agctttttac caggtatttt
    24181 ttaacttgtc tagtgatgaa cttagctata taacaaaaca atattatgat atgtttccag
    24241 aagatttagg tattcatgat agtgacatat ctttaatgat tacaggagaa agtataaagg
    24301 aaagaaataa gatactatta aacaatcttg tagatactgg atatatacca gctaaagatt
    24361 tagacataat caatgatata attatttctt tttatcaaaa tctattattc ttaaaaaaga
    24421 acacaggaaa aaattctgat gatgatgaat taactatgaa aatgatttct tctattgaat
    24481 acataataaa acatgctaaa taaaattaaa atatggctgt ctcaaaatag agataagcct
    24541 tttttattgt tgaaataata aaaatatatt taatttcata taaaaaagag tgcctcgtaa
    24601 actaaaaagt tcattttgag acacccctaa aataaattta ttatttatta tacaatagtt
    24661 tatatatcat cacctaaaac tacttcttcc atttcatgct ttttacttct catcattagt
    24721 tctattccaa cttctttagg atttgaacct ttctttatta cagaatatat agcattagtt
    24781 ataggcatat ctatatctaa tttttcagca acatcatgtg ctacttcagt agcagttatg
    24841 ccttctacaa ccatctttac ttcttttaaa gtatcttcta aactcatccc tttacctatg
    24901 agtataccag ctcttctatt tctactatgc atacttgtac aagtaactat taaatctcca
    24961 atacctgaaa gacctgcaaa ggtagacata ttagctccca tagcaattcc aagtctgctc
    25021 atttcactaa tacctcttgt cataagggca gcttttgcat tatcaccata ccctaatccg
    25081 tcacatatcc cagctccaaa tgctataata ttttttaatg ctcctccaag ttctactcca
    25141 actatgtctg gattcgtata aactctaagt ttagggctca taaataaatc ttgtatcatc
    25201 tgtgctattt ttaaatcttt agaagcaact actacggtag taggaatatc ccttgctact
    25261 tcttcagcat gagatggccc agatagtatt acatatggat tttgtggtaa ttcttcttca
    25321 cagacttgag ataatctaag cccagtccct ttttcaagac ctttagctac attaacaata
    25381 acttgtcctt cttttataaa tggttttatt ttttggcaag tacttcttac agcctgtgat
    25441 ggaactgcta aaactattac cttacaatct tttatagttt tttctatatc agtacttata
    25501 gttatattat ttggaaataa tactcctggt aaatagtctg tattttcttt agttctgttt
    25561 atttttttag cttgttcctc ttttcgagtc cacatgctca catcattacc tttttttgca
    25621 agccctaaag ctaatgcact tccccagctt ccagtaccta atacacatac tttctccatg
    25681 tttaacacca cctgagtata atatatttac tattctttct tttgaccaat ttttctctct
    25741 gtcccattta ataatcgctt tatatttgct ctgtgattat atattactga tgcagttaag
    25801 aacagagcta ctattaaacc agctttattt tttaccataa tcataaatat tggtgaacat
    25861 cctattccta caacagaacc caatgaaaca tactttgtta tagctaccac caatataaat
    25921 actgctagac acattagtgt tattactgga tttacagcta gcattgcccc tagtgaagtt
    25981 gcaacacctt tgcctcctct aaatcctaaa accgctggcc aattatgacc tgctacaaca
    26041 catataactg ccagatatcc agctaaaaga gtatctattc caaccaatct tgctgcaaat
    26101 tctgatatta aaacagccat tacaccttta agaacatcac ctaaaaatgt cattgctcct
    26161 gctctttttc caagtgtcct aagtacattt gtcgaacctg cattaccaga accttgagtc
    26221 cttatatcaa ccccagcaat tcgctttgca actatgtatg aggtagatat atttcctaaa
    26281 agatatgcaa ctacagcaat aattatataa ctaaatatct ccactgaatt tccccccttt
    26341 agttatattt taagatattt attttttatt tttttgtcta tactcaaact ttatggaagt
    26401 tccttcaaat ccaaaatttt ctcttatctt attctcaagg tatctttgat atgagaagtg
    26461 agttaaatcc ttatcattta taaacaatgt aattttagga ggtctaatgt cagtttgagt
    26521 cccatagtaa atttttaatc ttctaccttt gtcagatggt ggttgattta acattacagc
    26581 ttctccaatt acttcattca gtgctgaagt tgatattctc ttagaatgtt catttgacac
    26641 atattcaact gtatctagta tcttgttcat tctttgatta gtttttgcag aaacaaatac
    26701 gattggagca tacatcataa atggaaattt ttcttttata tcttttgtgt aattgctcat
    26761 agtcttatta tctttctcta ttaaatccca tttattgatt acaaatatgc atccttttcc
    26821 ttcatcatgt gctatacctg caacctttgt atcttgttct gttacacctt ctaaggcatc
    26881 tattactatc aatactacat ctgctctatc tacagcactc atagccctta ttacactgta
    26941 tttttctatt gtctcatata ttttactttt tcttctaagc cctgctgtat ctattagaag
    27001 aaatttctgt ccattttttt caaaataagt atcaattgcg tctctagtag ttcctgcaat
    27061 tggacttaca attactcttt cttctccaag tatcttattg agtatagaac tttttcctgc
    27121 atttggttta cctgttatag ctactcttat tatatcttct tcatactctg tatctaatcc
    27181 tgctggaaaa ttttcaacta tctcatctaa taaatctcca aatcccatac tattggctcc
    27241 tgaaactgca aaaggcgttc caaatcccaa ctcataaaaa tcgtatatat tatcaaattg
    27301 actttgacta tcaatcttat taaccactaa gattactggt ttttttgtct ttctaagcaa
    27361 ttgtgcaatt tctttatcag cagctgttat tccagcttta ccatcaacta taaaaagtat
    27421 tacatgtgac atgtccattg caagcatggc ttgatttctc atttgagata atattatatc
    27481 ttcgctatca ggttcaattc ctcctgtatc cactaatgta aaatatttat ctaaccattc
    27541 tacttcagca aatattctat ctcttgtaac tccaggtgta ttttcaacta ttgatatcct
    27601 ttttcctgca aatttattaa atattgtaga cttaccaacg tttggtctcc caactacagc
    27661 tacaactggc ctacttatac tcattttatt tctccttcca tcttttgttt tctattttaa
    27721 aattttatcc acaaaatctt ttccttcatt caaacaaggg ataattttta ttctcataag
    27781 ttcttctaat tgatttaatt caatattatc taagaaaatt tcttcatctg cttttagcat
    27841 acttcttgtt atataaagag cttctcctaa ttctttatct tcaagttgtt cttttaaatc
    27901 cttagctgtt aaaagacctg atacagttat tgtctctcca aaaaacttgt ttttgatttc
    27961 atatacattt acttgaagat ttttaaactt gtccatcata gcatctgcca tactctgtat
    28021 aaattcatat gcagaatgac cagtagctat tgatactttc ttctctcttt ttagaatctt
    28081 ctcaggcaat atatttagat actcttcaat ctctgttttt aacttacgta tcatacctac
    28141 accatcttca aactgtaaaa aaccttcata ttcttcatat ccaggcaatt cactattaga
    28201 tagtatataa aattcatctg ataaaaatgc aaatcgagta cctaattttt caagatattt
    28261 ctgttgtatt tgatgtattt gctctatagt tttacttgcg ctcttatcat taaatatatt
    28321 taattctact agattttctc tatgctttgt aatcccaact ggaactatcg caacactgtt
    28381 tacatacgga taaagttgag ctagttcttt tattgttcta tccaattcct ttccatcatt
    28441 tatacctgga cataaaacaa tttgacagtt catttctata tgagcatctg ccaatctctt
    28501 cattatacca tataattttc ctgcaaattt attgcttatc attttttgtc ttaactctgg
    28561 attagttgta tgcacagata tatttatagg acttattcta tattttataa tattatttat
    28621 atcttcttca ctcatatttg taagtgtgac aaaatttcct tgtaaaaaag ataatctcga
    28681 atcatcatct ttaaaataaa gagtttctct cataccttct ggcaattggt ctataaaaca
    28741 aaacatacac ttatttctac agcttttagc ttggtcaatt atcggatttg taaattcaac
    28801 acccaaatct tcatcatagt ctttttctat ctcgtatatg tagacttcgc catctttttt
    28861 ttgtatttct acatctaaat attcatcact tagtaagaat ctatactcta ttatatcatg
    28921 tattggctgt tcattcacag atataagtaa atctcctacc tctataccca tttcttctgc
    28981 aatactatct ttataaacct tacttataat attatttact ttttttacat ttacttccaa
    29041 tattttcacc actcttttgt attcatcaat gcttattata atgtatttta atatgcaatt
    29101 tatttattta ttatatttta tgatagcatt tttaagcatt tcatgtcata ataacacatt
    29161 ttaattctaa tagtcaatat aagtaaatag ctataaaata gattagaaca aataatattc
    29221 aaatttaagt ttcttaaaaa tttaaatatt atttgttctt aaaaaaattt tataaattta
    29281 atgttttact atgattaacg tcccatttgt catatcatga accattccac ctacacactt
    29341 tgacatcaac aatgccttat tttgtaagtc tttctcatca tttgcataaa atattggaca
    29401 acctccacca tttttcatat tcttatcaag tgtaactata gctagggtat attcatttaa
    29461 acctatatcc attcccatct catgcacttc ctttctttcc tattttataa ttttttagtg
    29521 ataagttttt tcctttagag cttgaaagta ttgggcatga ctttacagct tccattactt
    29581 tttccacatt cttatctact ggtaaataag caatcattat actttcatcc tctttatttc
    29641 ttctaggtaa tggtacgaat gaaggttctc cataatctcc atataaacct aaccttgaat
    29701 atatattgta tactattgct tgtctttgac cggagtcaaa taatattcct gcattagcat
    29761 aatccttatt tttaggaatt atttgtattc ctacacctct attaatatat acttctctat
    29821 cgctttttag acctatattt gttatacctt ttaaatctcc cacctttagt atagattcgt
    29881 ccacaaaact aatttcagca ggcattacat cagctatgtc tccaatactt tttctagtaa
    29941 gaagtttttt taatataaag gctaacacta atccacaaat tgaacttatt accaaactca
    30001 tcccttcact tattttcgtt tctgcaagta ttacataata caatcctatt gttaaaaaag
    30061 atgtaactat acacatataa tttcttactt cataggttct agctatctct tctatgaatg
    30121 cagctcctct ctttacaagt tgaacttcct ctaaattttg caatgtattt cttctatttg
    30181 ctctcacttg tctaaattgt tctgctgcta gagataaaaa agttatagat gtataagaac
    30241 gatttataag agctggaacc aataatgctc ctaatgaaga agcgacgaat gctaatataa
    30301 gttgagatag taaaacattt ggttgcgttg gatactgttt ttggtctaaa ttcaacatta
    30361 taattctaga tgttatacca ataataacag caacaaaaaa tactctatcc attaaatcac
    30421 tcctaaaata tttaatttga aattttacat gtaaatctac aaagtctttc tagaaaaatc
    30481 actctgcttt ccttttgatg tttctatgat aggtgtactt ttcactacat ctatcatgac
    30541 atctatatct ttgagtattg gcatatatgg tataaccaca gtactttttt ctaaattggt
    30601 cttagatata gcaacaatat caacctcatc tacatctcta tctatcccca tatgaataaa
    30661 tagattatgt attatagctt gtctttgccc tatatcattt actattccaa atgcacctaa
    30721 atctcttggt ataacttcta tggccagaca ttcatttcta tacttttctc ttgtactttc
    30781 tagacctatg ttagttatta ttacaccatt taccattaaa ataggtcctt caaaacttat
    30841 ttttgcagga actatatctg ctatatctgc tatactgttc cttcttaaaa atcttctaaa
    30901 aatcaaacca acaatagctc ctgacacaat agctaggatt gtgcaataaa aaaagctaag
    30961 gccatacttt cttgcaaaaa cgatataaac tatcgaagat accagtgcag aaaaaagact
    31021 tatataactt ctagattcat acgtagatgc tatttcttca acgtaagcgt ctccttttgg
    31081 tactaattct tcattatcga tattctttag tgttattctt tcttgttctg caagaccttg
    31141 aaactgttgt attgctactg caaaaaatgt caaggctgca aactccttat ctataagtgc
    31201 aggtaatgct atagcaccta acgaagctga aagcccagat ataattatct gttctaaata
    31261 atcttgtggt cttgatgggt attgtttgtc tgtaactctt aaaactaatc ctctacataa
    31321 tattcctaca actaatgcaa ctataaaaga gtgtctaaat aaatttgcat tcaataaact
    31381 ttcttccatt ttctcagctc cttctcttat atagtttgtg caaaaaacaa aaaaaattcc
    31441 cttctattta aggaaatttt tttgtaatat tgtaactata aattcgctac caatgccaag
    31501 ttcacttttt acttttatac atccatttag tgatttgact atatgctttg ttatagccaa
    31561 tcctaaaccc gttcctccta catccctact tcttgcttta tctaccctat aaaatcgttc
    31621 aaatatgcgt tctacatctt cttttggtat ccctacacca ttgtctataa ctttaattac
    31681 gattttttct tcatctttta acactacaac ttgtacaatc ccattatctt gggtatactt
    31741 tatagcatta tcaactaggt ttaagaacac ttgctttata tagtctttat tggatagtat
    31801 acttattgat ttatcatcac atttatataa aacttctata tttttttcac tagctgaatg
    31861 ttgagttatt tcatatacct ctttaaatac atcatatact acaacatttt ccataagaag
    31921 agtatcttta ttttctacaa atgataacag caatatatca tcaatcaacc tttttaatct
    31981 atctgattcg ctttctatta tttctaagaa tctatttcta gtattcacat ctatattttc
    32041 atttgacttt agtgtttcaa caaacccact tatagatgta agtggtgttt taagttcatg
    32101 acttacattt gcaacaaaat cacttctcat attttcaagt ttaacttttt tagtaatatc
    32161 ttctatattt actatcgaac ctattattac gttgttagta ttctgaagat aaactgggtc
    32221 tacttttatc ttatatacga tatcatcttt agtagtaata ttagcagttt cattatcttt
    32281 agaaccaata aattgaagta tttgttttaa tatttcttct tcttttatta caacattaat
    32341 attatgacct tcaactaatt gatttggttc actctttatc atttcttttg cttcatcatt
    32401 tatcagaagt atatttccat ttacatctat agccaaaatt ccatgagata tactctttaa
    32461 tatagacctt aactgtaaat gtttatactg tacttcttct aaggttttat ccatattctt
    32521 tatcatatga ttaaaatttc tagctaattc gccaagttct cctttggctg atatgttaag
    32581 cctagcatga tattcattat tacttacttt ttttgagacg tatataaact cttttaaata
    32641 tttccttaat gttattgtgt atcttacagc taatataaca atggctatgg acaacataac
    32701 aataaaaaaa tatatactat ttaaatcttc cacaagataa cactcctaaa atataacctt
    32761 tttaatctat tttgtatcct acacctctta tagtctctat atatttttcc gatttatctt
    32821 catcttctat tttttttctt aaatatctta tatgaacatc tactgttcta gtttctccat
    32881 agtattcata cccccatatt ttatccaaaa gataatttct tgaaagtact tttcctttat
    32941 tttgaattaa taattttaac aattcaaatt ccttaagagt taactctatc ttttcagagc
    33001 ctttggtaac ttcatgtttt gataaatcta gttttaaatt acctgtagta agtatattgt
    33061 tttcttcacc taagctagtc aaattatatc tccttaaaac agaactaatc ctagctaaca
    33121 actcttttat actaaatggc ttagttatat agtcatcagc acctatttca agcccttcaa
    33181 ccttgtcatt ttccatattt ttagcagtaa gcattataac aggaatattt tttaagtcct
    33241 tatcacttct tatttttctc aaaacttcta ttccacttat atttggtaac atccaatcta
    33301 aaagtattaa atctggttta atttccttag cctttataaa tccgtcaaat ccatcatatg
    33361 aataactaac ttcgtaattt gacacctcca aattgaattt taatagctca actatatgca
    33421 tttcatcgtc tataacaagc actttagtat tcattaaaaa gctcctctct attttatctg
    33481 tagtaccgtt cctattcgtt ctgatgcctt attatgtttt ctataagagt ggaatttatc
    33541 tgctctacaa ctagtgcata aatttaaatt tattatattt tcttttttaa ctccactaca
    33601 tctaagcata tactcattaa ttttccataa atctaaatag taacttccct cttttattat
    33661 atagaacttt tcatcattat ttgtaagaat tgtgttaaat ttttctacta aatcttcaga
    33721 cacttcataa caacatggtc caattgatgg cccaattata catactaaat cttcaggaac
    33781 tgttttatag cattcagaca ttaattttat tgttttatga cctatattac taaaagtccc
    33841 tctccaacct gcatgagcca gtcctatggc tttattttta gcatctataa ttgatatagg
    33901 tacacagtct gctgtaaaca gtaaaagtgg tactttttcc aagtttgtta taagtgcatc
    33961 tccatctctt cttttcccta tagtatcttt attgactata ttaactatat ctgaatgtat
    34021 ttgagaatta ctagttagat tttcaaatgt caaatcacat tctttacata cttttaacat
    34081 atcttctcta ttttttgcat ctatattctt acatgttata actagattta cagaatttaa
    34141 tttttcatca agttcttcac aattaccact taaattaaca tactctttaa tatcttgtac
    34201 attttttata tttatataat ctttcatcat ttgtctcctt tttctatcag tatactttta
    34261 agttcattta caaaagtatt tatatcttta aattgtctat acacagaagc aaatcttaca
    34321 taggcaacct catctaactc tttcaattta tccattacca tctcaccgat ttttttggtt
    34381 tcaatttctt ctataaaaca tttatttatt tcattttcaa tatgtacaac aatactttca
    34441 atctcgtcaa tagaaacagg tcttttttca catgctttta gcaatccata ttttatcttt
    34501 tctctatcaa aatattctct actattatcc tttttaatga ccattaaagg agttgtttct
    34561 attttctcat acgtagtaaa cctcttttta catgattcac actcacgtct tcttctgata
    34621 gattttaaat ctgtatgtct agaatctatt acctttgatt ctttataatt acaataagga
    34681 cattgcatct atattcctcc tttgtttaac tatatctcaa actcatctaa gcttaattct
    34741 gaacctatat ttactattat tgtatcacaa cctattttaa gtatatcatt ccaaaatata
    34801 acttcttcat cccctcctct tgaaaaaaaa cttctacttc catctccaaa aatggtaaat
    34861 gagactacct taccttcgtg aatatccatg tctatgtcta ttataaaccc catccttttt
    34921 ccatttttta catttataat ttctttttcc attatatcag aaactctaat catattgtac
    34981 tcaccctttc tttaaaaata aatatgtatc gaagtaaaaa aaaatgacct atataggtca
    35041 tttaaattaa aataatgttt acagtccatg gattacttat acatattttc tcatattttt
    35101 taaagcattt ttttcaattc ttgatacttg tgcttgagat ataccgattt catcagcaac
    35161 ttctatctga gttcttcctt tgtaaaacct taaatctaat accaactttt ctctactatt
    35221 taatttctta atagcttctt taagtgatat ttcttgcaac caattttcat cagtatcttt
    35281 tttatcttga acctgatcca taacgaatat tgcatctcca ttgtcttgat atactgggtc
    35341 aaacagagat attgggtctt gtatggcatc aagtgccatt acaactgatt ctacttctaa
    35401 ttctaattcc tttgctattt ctgatactgt aggttcttta gaatttgttc taattaatct
    35461 ttctctaact tgtagtgcct tatatgctat atcttttaat gacctactta ctctaattgg
    35521 gttattgtct cttagatacc ttctaatttc tcctataatc ataggaacag cataagttga
    35581 aaaccttaca ttttggctta aatcaaagtt atctatagct tttataagac caatacaacc
    35641 tatttgaaat aaatcgtcta tattttctcc tctgttgtta aactttttta ttacactcaa
    35701 gactaatctt aaattccctc ttacaaactg ttgtctggct tcttcatcac cattttttat
    35761 ttgtaataaa agttccttca tttgcttatt tttaagaact ggaagttctg atgtattaac
    35821 accacatatt tcaaccttat taacttgcat atattttcag tcctctctca aaagtattca
    35881 tctattgaaa ttatttacat ttccaatttt tttatacttt ttaagaagaa ttaatttagc
    35941 attatacaaa ttttttcatt tctttttgca accttgagat tattttcttt tcaagtctag
    36001 atatgtagga ctgtgatatt ccaagcattc cagcaacttc tttttgtgtt ttttcaatac
    36061 ctttatcaat aagaccaaat cttaattcca taatttgctt ttctctgtca cttaatctgt
    36121 caagtgccat aaccaacaaa tctctatcta tttcttcttc aattattttg tatatttcat
    36181 cattttctgt acccaataca tcagataata aaagttcgtt tccatctaaa tctatattta
    36241 aaggttcatc aaatgatact tctgttttct ttttattatt ttttcttaag tacattaata
    36301 tttcattttc tatacatctc gaagcataag ttgccaattt tatattttta ttcagtttaa
    36361 aagtattaac tgcttttatc aaacctatag ttcctattga aatcaaatct tctacatcta
    36421 tacctgtatt ttcaaatttt ctcgatatgt agactactaa tctaagattt ctttctatta
    36481 aaattgactt tacactttca tctgtttcta gcttttgtaa aagctccatt tcttcttctg
    36541 gtttcaatgg aggaggaagt atatttgccc ctcccatata gtatattccc tttggcttta
    36601 ttatttttaa tttaatacct aacatagtaa taaagcttat tattctttct ttcaatcgta
    36661 acattccttg tcccccttaa attttcacct aaaatccagg caatatattt ggattcatta
    36721 ttgctccata ttctctatca ttaaaatttg aaataccaag tattatattt cctatctttt
    36781 gctcatctac ttctatatag tcagctttta agcctattat tatgcttgtc ttattacttc
    36841 cagcatgttt ataaggtatt attctaactc tacctgaagt tttatcatct aaacttccta
    36901 taatttcttg tgctttcttc atatctatat cttcataatt aatatttaat agtttttctg
    36961 gaataaatcc ttctaacaca ctagacttaa ctattattac atcactttga cttattgggt
    37021 cttttaacaa attaccacta tctactaaag ccttacagca aaaacttttt tctagtaagt
    37081 ttatatttat agtctttgta agctccttaa tgtacttaag cattttcaaa tctttataaa
    37141 tgtattttaa taattcacaa ctcacataag tacatgcaat taagaaagat atttttaagt
    37201 gacttattcc tgtaaaataa attataaaat aagtgcttcc agaaataaaa atgtttacta
    37261 gataaaaaac taacgctaca tgaacataat cttttttgct cttacttcta aatgaaatta
    37321 aagttattat actcatgatt attattttaa atggtacagt aaatagtata ttcattgatg
    37381 gatacaaata agcaacagaa taggcacctc ctaaactagc ccccaataac ttattttttt
    37441 taggattata tcttttagta agaatagaag tacaacttat aataatataa tttattacta
    37501 ggttttctat aacatagtac tcaatataca ccatgtatcc ccccttaaaa cctttaaaca
    37561 tattataaga tagtatgtac aaaattttat gtcacttttg atggtcgaat ccaaaacaaa
    37621 ttctatttaa aaagaacaat gatatatgtc ttatacttta tgtcgtcaaa agatattttt
    37681 ttaataccaa acaaatagac ttttttgtag aatccaatat atctaggtag aagttattca
    37741 ctctactttt ggtatattta caaaaaaaga ctacataatt taataatata tcaagtaaat
    37801 cttttactta tataaaatca aattatatag tctgtaaatt caaatataaa ttattgtgct
    37861 agtaatccat ttgcatattt tgcagattca tttagtgctt gttctggagt tatgtctcca
    37921 gctatcatca tttgaagttg tttccataaa tattcatata tctctactcc atgaggtcca
    37981 actgtcaatg gtctatacat atgtttatct cttgtcacca tttcttcaaa tcttgggtct
    38041 ccttggtatt tttcaccttt agcaactgga gctctaggta tttctttgtg gaaagcttcc
    38101 attgattctt tgcttagcat atactcaaga agtttaaatc caagttcttt atttttacat
    38161 gctgacatta atgttaaatc atctactgaa gcaaaacttc caactgtctt atcttctaat
    38221 gatgttacat atccccagtt taaatccggg aatgatttat caaaagtact tgtagcagca
    38281 gaggatagca ttatagtaaa tgcagctttt cctggtccaa atacactttc aatcatttca
    38341 ttactatctt ttgataaagc atcttctgga atatactttt tcatatcatt tataaagttt
    38401 gcagctttca agccttctgc attattaaat ttaactgatt ttaaatcatc attatatata
    38461 tctccaccag cttgccataa atacggatac cagttccagt ttaagcttcc aaatacttta
    38521 gaaccccaac cttgtgcaag accccactgg tcgatttttc catctccatc agtatctttt
    38581 gttgcttttt cacagattct tttaaaatca tcccatgttt ttggaggttt ttctcctaat
    38641 ttttctagta tatctttatt gtagtacaat acgcctggat tagcagcttc tataggtaaa
    38701 ccataaatac cacccatcat ttttgcatca tctaagtata gataattatc tgaagttcct
    38761 gatttttcta aataaggtgt taaatcttct accgcaccca tttctataaa ttgaggaaac
    38821 atctctgcat acatgtatcc tatatctgga ccttcacctg cacttattgc tgttgcatat
    38881 ttttctgcgt agttatccca tggtactatc tgaaattcaa tttcacagtt attttctttt
    38941 tcaaattttt caaatatcgg cttccatgca tctgcatcat ggtctgttaa tggtgcagac
    39001 catacaacta atttttcttt accctctttg cctttagaat cttctttttt tgaacatcct
    39061 gcaagaattg atactgatag aatccctacc ataactaaag aaagaacctt ttttagttta
    39121 atcatgctta atcccctttc agactttaat ttgtcttttt cctcaaatgt ttacatttaa
    39181 ttcagattta atattaacaa ataagattac tcttgtctaa tcataaaaaa ctatactaac
    39241 tcctataact atattttttt tctataccta ttagatttaa ttcatactaa aaacttactt
    39301 tttaaatttt atcaatattt aaaaattttt ttaagtctag ccacaaacta actatctaaa
    39361 ttgagcaaaa taaaaagaag tctattgact tctttataag cttacatatt atcttcttct
    39421 tcttaggaat gtaggtatct ccatgctctc atcatcatct tcttcaatta tacttgcctt
    39481 tttaggaggc tctatttttt cctcagcaat tacttcttct tcaatttctt ttacagcttg
    39541 ttttactgtt gtatttaatg aagaacgtat gcttgattta ggtttagtat ctaaatctaa
    39601 ggcaccattt tgtaatcctt caaatcctgt cgcgattact gtaatcatta tttcatcacc
    39661 taaatcttct tttatagaag ctccaaatat aacatttgct tctgggtcac aggattccat
    39721 aactaaagtt gatgcctcat taacttcaaa taatcctaag ttaggaccac ctgtaacatt
    39781 aagaagtact cctttagctc cttgtattga tgtttctaga agtggagatt gaatagcttg
    39841 tcttgcagca tcaattgctc tagtttcacc actagcacta cctataccca tatgagcaag
    39901 tcccttgtct ttcattatag tagtaacatc tgcaaagtcc aagtttatta acccttcaac
    39961 tgctataagg tctgaaattg attgtatacc ttgttttaat acatcatctg caactgcaaa
    40021 agcatctaac attgatgtat ttttttgtac tatctgtaaa agtctatcat ttggtatcgt
    40081 tataagagta tctacttttg atttaagttc tgctattcca ccttcagcgt tcttcattct
    40141 tatcttacct tcaaatgcaa aaggtttggt aactacacct actgtaagta tccccatttc
    40201 ttttgccaag cctgcaacca ctggagcagc tcctgtacca gtaccaccgc ccattccggc
    40261 tgttacaaag accatatcag ctccttgaag taactttact atttcatctt tactctcctc
    40321 agcagctctt ttccctactt ctggatttgc tcctgctcca agtcctctag ttaatttttc
    40381 tcctatttga actttatatt cagcttttga tgtatataaa gcttgtttat ctgtatttac
    40441 tgatataaat tcaacgcctt ttagttgagc ttctaccatt ctattgacag cattgtttcc
    40501 accgccacct acacctatta ctttaatttg tgcacattct tctaattcta cgtcaaagtt
    40561 tagcatcata aacccccttc tctcatgatt caaagttatc gtatgtatta attatatcat
    40621 tttcttcttt tattttgtta acctttaagt aacatattcc acaaaaacgt aaattttcct
    40681 ttttaaattt cttacttata ttgtcaaata ttactcttta agtataaaat aatttacgta
    40741 tatattaaat gctctttaat ttttacgttt tttgttcata atattactaa taatatatcg
    40801 tctaattata gataagttat taaacaatct cataccaaaa actaatacag caacatagta
    40861 aagagaaact cctaactttt ctcctatata agtcaataat atagccaata tagcattacc
    40921 tatgaaacca gtaataaaaa ttacattatc atagttatct tcaaaattgg attttatcgc
    40981 tccaaaaata gaatccatta ttgcaagaat agctactgac atatatatgg aataatccat
    41041 agaatatgta aatggaatat agtaacctac tactacacca aatgctaatc ctcctaaaac
    41101 ccataacata aatcttcacc tctttatttt actttgaaac acaactacat tacaatcctt
    41161 tttattaatt ttttttactt tttctttatt tttttgtatt tttcgtttta ttttgcaaat
    41221 aaatccactt tatttccatt attttcctga acttttatat taattccata tatatcctta
    41281 agagatttag catatgattc tggagagttt atagctgcac tcaataaatc tatatctcct
    41341 atagctttta ttataaatgg ttgtccatat gtagtatcat ttactgtaat agtagctcct
    41401 gaacatttta tcttacttgt tgctagtact ctttctccat ttatagaaat tgctcttgct
    41461 ccagcctttt ttaaatcatt taaaaccctc aaaatatcta tatcatgtat aatcaaatcg
    41521 tttggattct gtccgtcttt taactctctc tcactatccc tcatagtaat agttatgcct
    41581 ggccctgtaa cagttgaata accacttaat tctttaactt cttcaagctc tttttgcata
    41641 agtgtttgga tagattcatt tccattcttt tttgcttctt tatattcatt caaagttttt
    41701 ttgttgtttt taagagtttc ttttaaattt tttatttctt tttttgtatt ttctacctca
    41761 ctttcaatac tttcagtttg acctaatgtt atgtatactc tatttgggtc tagtgatttt
    41821 atataagttg atatgaaaac gcctaatatg aatgctccta atataactat tttctttttc
    41881 atactattat ttcccctctg ctttgtttat atcactatga ccatttattt ctatattgtc
    41941 ttctttggtc acttttactt tggcattata atatgtttct atttcccaga caataccata
    42001 ctttatatta agtgccgaag ccaatgtatc agaatcacca attgctttta ttataattgg
    42061 ttcttttatg acagtgtcat tcatataaag cttgtcttct tttaaataca tatatgtatt
    42121 agaaactatt ctctggtcgt ttatagatat ggcacttgct tgtgctgaat ttaatttatt
    42181 tatcattgac aataataaat cataattata tagtatacta tcatttttat cagagtttcc
    42241 taagtctttt gtagaattta tctcaatttt tatgcctgtt ccttcaacat ctgtatatcc
    42301 agctaatgtt tcatattttt ctatttctga cttaagtatg tcttgttctc cttcattgtt
    42361 tttgcctcta taagtctcta tatctttctt cacactatta acttctgatt gtagattctc
    42421 tttttctttt tttaaagatt taagttcttc tgtaagttgc tctcctcttt taaaagttgt
    42481 catccctcta ttttctatat taataatttt aagttgtaaa ctaacaagaa cacccaatac
    42541 aatacttcct atgattatac ttttatttat aaatcttttt ttcaatatat cacttcctta
    42601 caattgtcta tccttctgga ctatacactg ccaactttcc atagttaaga tttatagtcc
    42661 ctccattagt atttttattt tgcaggtcaa tcagaatttt acttacccta gatatattat
    42721 aatctaagtt atcatcatta gaaagtaaga tttttatatt acttcttgta agcatttcta
    42781 tattgctttc tttttttaag tttacataat ttatttttct ataaattcct tcttctttta
    42841 aataatttaa agttttaaat acattctcct tagttttata atttttaaat tttatagatt
    42901 tattatcatc aatgctataa tctacatcta ctatcaagtc ttttttactt tcattagaac
    42961 ctcttagttc ttccaataaa tttccttttt tatctatata acaataatta tcttcatctt
    43021 ttaatactgc aaatatttct ttttctttta aacttataat tatcttattg ggaagttttc
    43081 tttttatttc cacattttct atatatggat tttttataag tttatttttc atatctttaa
    43141 aattgtatgc aaaaatattt tcatttagat ttacatttag ttctttcatt atatttgatt
    43201 tggtcaccct cttatttcca attacatcta ttttctttac atcaaataaa tcacttttta
    43261 caattataca tatacaaaaa gataacataa gtataaaaac taaaactatc atgacattat
    43321 tcgtatttaa ctttcttctt tttttcaaat ataatcaacc cctttggtta ataaatacac
    43381 ctttagagct tgtttttcct acaaaaaaat tataatattt aaagtcaaat ttttgcattt
    43441 tattacataa catccaaata aggtattaaa ttttataaat aaatcaatat ttcatcaaaa
    43501 taaacatagc atactacata ctatttatat atttaaatct gtatacatac aaataaaccc
    43561 ctaatttata ataatttaga aaatctaata tattataaat taggggtaaa tatttactta
    43621 tcttttcttt attcctatta ccttagcttg accttctata cttggagtcg tttctttttt
    43681 tatttctttt acttccttaa taactttttc tttttttgtt tttttagaag ttgacttttg
    43741 tgttgaattg tatactttca ttatctcatc atatataagg tctatggctt caggcttacc
    43801 tatagtttta cttgcatttg ccatatcaac caaaagttct ctatctccta aaagtttaaa
    43861 tactgctgta tttaggctct ctggagttag atttttttct aaaatagcaa ttcctgcccc
    43921 ttgtttttct atactcttgg cattatactc ttgatgattt tcagcagtat aagctttagg
    43981 tattattata gatggctttc caagagccgt tatctcagct aatgaaattg ctcctgcgct
    44041 acctataacc aaatcacttg ctgcaagtgc atttgccata tcttctaagt aaggcacaac
    44101 tttttggtat ggtttaagat ttatatcgct aatacttccc ataaattcat cataatagct
    44161 tttgcctgta gcaaatataa aagctatatc ttcatttacc atattcttta ttacaagtct
    44221 cattgcatca tttatttttc ttgaacctcc actaccacca tagcaaagta ccattctttt
    44281 ttcgtcactt attgatagat tctttcttgc aatatttttt cttgataata aaatttcttt
    44341 tctaactgga tttcctgtaa aaactaattt atcttctgct gcctctggaa atcttttatg
    44401 cgaatcttca aaacttgtaa gtacctttgt aactgtttta gataaaatct tattagttac
    44461 accaggaaaa gaattttgct catgtattat ggctggtatt ttccccatag atgcattaaa
    44521 taaaacaggt ccactaacat atccaccagt tcctataact atatctggtt taaatttctt
    44581 aacaatcttt cttgattgct ccaaaccttt aaaaagctta aaaactcttt ttacattatc
    44641 gaaatctatt tttcgcttaa aaccttgaac tgttacagtc tttaattcaa atccatactt
    44701 aggaactatt tctgactcta ttcccttttc cgtcccaaca aaaattatct cagcgtctgg
    44761 atgctcatct cttattttgt ttgcaatagc tatagctgga tacacgtgcc ctccagttcc
    44821 accgcctgat aataaaactt tcatcaaatc atcatctcct gttaattaat tttgacatgc
    44881 tttgagatgt ttaacactat tcctacagct cccataaaga tggtgagtga agttcctcca
    44941 taacttataa atggtagtgc tacacctgta gctggcattg atgatgtcgc aactgctata
    45001 tttaaagctg cttgtattcc aatttgtgca cctataccta taacaaccat acaagcaaat
    45061 acattagaag tcttcaaagc tattcttaca catctgtaga ctaaaactac aaatagcata
    45121 ataactatta tacaaccaat caatcctaac tcttctccta ttatagcaaa tataaagtca
    45181 ttttgtggct ctggtatata aaaatacttt tgtttactct ttcctaagcc tagaccaaac
    45241 aatccacctg aacctagtgc atatagacct tgtattacct gatacccctt accaagaggg
    45301 tcttgaaaag ggtctaaaaa tgatgtaaca cgactaagtc tatagggttc agcaattact
    45361 aatgcagcaa ataaagcagc cccactccct atcatagcaa atacaatttt catatccatt
    45421 ccagcaacaa atatcattac aaaagtaact agaattacag tacctgctgt tgacaaattt
    45481 ggctgaagta ttatcaacgc aaaaaatatc cctggaacta cgagaagtgg tacaacacct
    45541 tttgtcaaag attttatttt atcataattt ttttcaatta attttgcagt taatattatt
    45601 gtagcaaact ttgcgatttc tgcaggttga aatgttaaag ctcctattcc caaccatctt
    45661 tttgctccgt ttgcttctat ccctaaaggg gtcaaaacta acaataataa tactactgct
    45721 atagctccaa ttgctgtagc atttttcttc caaaaactat aatctattct tgatgtaata
    45781 atcataacta taaatccaag tacagcatat attacattct tttttagaaa atagtacgca
    45841 tcattgtgtt taaatgaaga ttgtataaaa cttgcactaa acaccataac tattcctaca
    45901 aatactaaaa gcatagtagt gtaaaacact actccatcaa attcagtctt cacccttatg
    45961 tcaatttgtt ttttcaaatt ttctttaggc atccaactaa tctacctcca ttcttccatt
    46021 taagtaagtt tagaggtaag agattatttt aaattgttta cattatcttt gaagtctttt
    46081 cctcttactt caaaactttc atacatatcc caacttgcac aagctggtga aagaagcact
    46141 atatctccac tttttgaaat ttcataagaa gtcttaactg cttcttccat atcttttact
    46201 atatgtatgt cattaaatcc tttattttta gcacaatttt ctatatttga tgcagtttct
    46261 cctaataaga ccaatgattt aacatatgac tttgcagttt caagaagttc atcaaaatta
    46321 cttcccttat ccatcccccc tgctataagt attataggtc tatcataaga ctgtatagct
    46381 tttatagtgg aatctggatt agttcccttt gaatcattta catatattac attgtctaaa
    46441 tttcttacaa actcttgtct gtgttccact cctttaaatg tcataagtac atcctttata
    46501 acttctaaat caattttgca gacatatgct attgctatag ctgccataca attttctaag
    46561 ttatgacctc caggtaaact tacatcatct ttatttagaa atttgatttt atcatctata
    46621 tctattatta tgtcattatt ttctaaatat actcctccat ttactttttt agttcttgaa
    46681 aagtaaatta ctttggcatt gcatttatca gctaaagatt ttactatctc atcatcatag
    46741 tttaatacac agaaatcctc aacagtttgg ttcataaata tatttgcctt tgcttctatg
    46801 tatttttcca tagtatggtg tctatttaaa tggtcctctg taatatttaa tattgcgctt
    46861 actttaggtc taaattcatc tatactctca agctgaaaac tgcttagttc agttactaac
    46921 acagactctt cacttgaagt ttctatagta tctattacag gatttcctat attaccaact
    46981 acatatgtat ctcgttttgc tcttgaaaaa atttctccta ccaaactagt tgtagttgtc
    47041 tttccatttg tacctgtaat ccctataaaa attggattat ccgatagttt aaaagctaat
    47101 tctacctcac caattatata cttgcctgaa ttttttaatt tcaatatgaa tggtaggtct
    47161 agtggtacac ctggagacac tacaaccata tctatatcat ctacatcttc tgggtgatgt
    47221 cctaatatgt attttatgtc atttatagat ttcaattcat caagtatatt tctcaattta
    47281 ttttcatctt ttatatcatt tacgattata cttgcaccca atttatctaa atgtttaatt
    47341 gtagatatac cagtttttgc aagtcctact aataaaactt tttttccttt taagtccatt
    47401 tttcattccc cctagcccta aaatactgct attatactta tccaagctaa aacaactgtt
    47461 ataatccaaa aaataaatac aactcttgtt tctggccaac cacactgttc aaagtggtgg
    47521 tgtattggag ccatcttaaa gaatctcttt cttgttgcct taaaatatcc aacttgaatc
    47581 aaaactgata gagcctccgc aaaataaatt cctccaatta taggtattat aagcactgag
    47641 ttagttagaa ctgaaaatgc tacaactgca cctcctaatg ccattgaacc tgtgtcgccc
    47701 ataaacactc tagccggata tgaattaaat cctaagaaac ctaaacatgc tccaacagtt
    47761 gctgctgcta aaactgcgac ttctgtattt ccagcaatag aacttgcaaa tagcataaag
    47821 aatacagaaa ctattaatgt tactcctgat gctaatccat ctaatccatc agtcaagttt
    47881 acagcattta ctattgctac gattataaac atcattattg gtatatataa tattccaaca
    47941 tttatcacaa aatctgtaaa tggtatcata agttgtgaag cacttgatga tgatgtatac
    48001 tgataaaaag caacataaaa agataatgca acttgaagga ttattttttg atatgctttt
    48061 agtccaagtg atctttttag ctttattttt ataaaatcat ctaaaaagcc aataaaacca
    48121 aaacctgcaa tacatataag acctactgcc atatcatgac taacttttac tcttgtaagt
    48181 ccagttatta gaattgcaac aatcattata attccaccca tagtaggtgt tccgttttta
    48241 gctagatgtg tttggggtcc atcatctcta actgtctgac caaatttaaa ttttctaagc
    48301 attggtataa atattggtcc tattataatt actattaaaa acgcaatcaa tgcggtgtac
    48361 gtaagctctg ttattcctaa catcataaaa ctcctctccc ttgtcaatta actcattagt
    48421 ttaagcttat ttatttagat attcgactat tttttctaat ctcatgcctc ttgagccttt
    48481 tactaaaata gtgtctcctg tttttactaa ttcatttaac ttgttaaaca catcttctct
    48541 attctcaaag tgatatatat tggctgaatt gaatcctaat tgttttgctt cttctccaat
    48601 aaacactgaa ttttcaccta tagttattat aatatcagta ttattcatag atgattttcc
    48661 aactaatcta tgaccatact ctgaaatttc acccatttct agtatatctc caagtatcgc
    48721 tacccttcta cctttatatc ttccaagtat ctttaaagca gcatccatag aatctggact
    48781 agcattatat gaatcattaa ttattgttaa tttttcattt tttattatgt ctagtctcat
    48841 ttttgtccct ttaaaatttt ttaaaccttt ttttatataa tctaaagaaa tatttagaca
    48901 caaaccaact aatatagcag acattgcatt atatatatta tgttctccta ttgttggtat
    48961 aaatatttcc tcttttttcc catctattac acatataaat gttaaactat cctcttccat
    49021 agtataactt tcacaataaa catcattgtt tttattaaag ccaaatgttt ttaacttata
    49081 tacatgttct ttttctttta atgtagataa aaacttatca tctccattta caattaaagt
    49141 atttgtttca tcaaagtttg aagtaatctc catctttgct ttcaaaatac cctcttgtga
    49201 acctagtttt tctacatgtg ataatcctat attcgatatg actgcaattt taggatttac
    49261 tatatccact aaatatttta tttcattgaa tccagacata cccatctcta ttacagcaca
    49321 ctcatgttct ttatttagat taaataaagt caatggaact ccaaaatgat tatttaagtt
    49381 tttttcattt tttagtatat ttaactttgc agatatggca gagtaaatca tatctctagt
    49441 agtagttttt cccacactac ctgtaacacc tataaatgga atatcaaatt tttctttata
    49501 gtattttgaa atatctccta gagccaaaga tgtatctttt acctctatta aatttatatc
    49561 cgaactttct aattttattc cattactttc attttttata aaagttttac atccactatc
    49621 atatgcagac ttcataaatg tatgcccatc taaattttct ccaactatgg ctacaaatgc
    49681 attttgggca tttgcctccc tgctatctat aactatatca cttacacagt cattataatc
    49741 tccatatatt aacttcccat tagttgcaag tactaactct tttattgtta agcactccat
    49801 tattaaactc ctcctttaaa accttagcta tattctctta gcaacaaaag gcaatgtata
    49861 ccgttatata tacattgcct acgataactc ttaactttat aattatatac tatatttctg
    49921 tttttttgaa gatgtttatg ggtatatgtt aattatctgt tactttttag ttttcaaaat
    49981 atagggttat acttgagtct tcattaactt ttactccagg ttttgggaat atatccttta
    50041 cctttgtatc ttctggaagt tctatatctg catctaagtc tggttttaac ttcgcatcct
    50101 ccaatgcctt tacagcatct cctattttta gatttcttac atctggcact ttaacttgct
    50161 tcttttcata ctcagctttt tcttcttctt tatatactgg tttaactccc aaatatttta
    50221 aagagtcatt cattatttct ttcactattg gtcctgctgt agtactacca aatgcactta
    50281 ctcctgttgg ttcgtcaaca atagcaagaa ctactatttg cgggtcatca caaggtgcta
    50341 ttccaacaaa tgaacatata tactttcctg gagcatactt accatctata actttttgag
    50401 ctgtacctgt tttaccacca agtctataac ctggtatata cgctatcttt cctccacctt
    50461 cagttacaac tgattctgca atttctaaca tttttttaga tgtttcttta gatataacac
    50521 ttcttacttt ttttggtttt actgtttcag ttatatttcc tttattgtca gtataagatt
    50581 ttaccactct tggttgcatt aaatctccac cattagctat tgatgatata gctgttataa
    50641 gttgtattgg agttactgat atagattgac caaatgatat tgtagccaat tcaactggtc
    50701 ctacattttt ttcgttatac agtattccct tagcttctcc tggtaaatct atacctgtct
    50761 tatccataag accaaatgat tctatataat catacatttt tccaacacct aatctgcttc
    50821 ctaactcaac aaatactggg ttgcaagagt tttgtactgc ttgtttaaat tcttgtgttc
    50881 catgaggtct gtaatgtctc caacatttaa tctttcttcc tccaacagtt acactacctg
    50941 tacatgtgaa tttttctcca tctttaatta ctccttcttc aagagcactg gaagatgtaa
    51001 taagcttaaa tgtagaacct ggttcatatg tatcacttac tgctggattt ctccacattt
    51061 gataatagcc ttttatttta tctttatcat tatatttttc aagttcttct tgataatatg
    51121 gatatattgg cgtccttgag tcattagggt cataatctgg ttttgatgct aaagcaagta
    51181 tatcacctgt tttagggttc atagctattg cagtaacctt ttttgcatta tttaactcat
    51241 aagctttctg tactgctttt tcagtatagt gttgaattac ttcatctata gacaacacaa
    51301 gaccattacc ttgtacaggc tcataatatt tttccattcc ttgtggtatt tctcttcctg
    51361 aagcatcagt acttactata agttttccag gctttccttt taacttttta tcatattgca
    51421 tttctacacc agatatacct gttgcatctg aagaagtatg tcctaacaca tatggtgcaa
    51481 aattcccata tgggtaatat ctttgattat cttctgccac ccatattcca cttaatttag
    51541 catctcttat ttgacttgcc ttatcatcat ctatccatct ttttactttt acaagagcca
    51601 tatttttttt gcttattaaa gcgtaaatat ctttataatc ttcatccaaa atctcagcta
    51661 ccttttcggc tgcttctttt ttatcttcta cttccacagg tttacaccaa acagtatact
    51721 tagttacact tactgctaac tctttcatat ttctatcata tatagttcct cttttgggtt
    51781 ctattggtat atctcttgtt tgttgttcta gtgcttttgt actcaaccaa tttcccttca
    51841 taagttgtag atacccagtc ctaataacaa ggcaaaaaaa caatgcacat gctagaataa
    51901 gaacgagtac cagccttttc ttacttatcc tctttacttt tctcaaatct attcccccta
    51961 gttatccaca tctatatact ttatttgtcc atcttttgga tagtccatat tgagcaattc
    52021 cttagccttt agctcaattt cttttattga tgttttttca gtttcttttg cctttagttc
    52081 ctctaatact atctcttttt ttcttaaatc tttttgtaaa taatagatat cgtacttcat
    52141 ttccgagatt tttgaataac cacatagatt caatattatt actgaaaaag ttgcaatcag
    52201 taaaatgcat ttagctccta tttttttctt atttctcttt ttataattat tctttttttt
    52261 atttttatat tcatttagat tatgtatttt ataatttttt ttcaaattct caccccgata
    52321 gtatatagtt taaattttcc tctatactat atttgttatt aatcatattt cttcctctaa
    52381 aatttcatcg ctaaattaat acactattac acaatctaat tgtatttttt tctatacaca
    52441 acaaaaacct ataaattcac ttatactttt atatgaatct ataggttaga tattaatttc
    52501 tatttatttt tatcacttct agtattttta gataatttct tccataaata taatatgtaa
    52561 ctaactatct actctctgat tcctaagttc tccattatat tacttaatac catctatatc
    52621 tatattttta tatctaaatt ttcattatat atctcaataa caaagaacat ttcttatagt
    52681 gttaattgtt ttttaattgc tttaccagtg caacaaaata tataatactt tttaattaat
    52741 tataataatc ttatatataa gattattctt tagccttata actacttata ttttttctgc
    52801 tactcttagc ttagcacttc ttgaacgtgg atttacttct atttcttcct cactaggtaa
    52861 tataggtttt ctagttatta ttttcacctc agattcttta tcacattggc atataggtaa
    52921 atgctgtgga catatacaat ctgatgctaa atgcttaaat gcatttttca ctattctatc
    52981 ttctaatgaa tggaatgtta ttatacatat tcttccacct tcattcatta tagaagaagc
    53041 atcatttatc atctttgtaa ttactccaag ttcattattt acttctattc gtattgcctg
    53101 aaaagttctt ttagctgggt gaggtccatc tattctggct ttcttaggga tagctttttt
    53161 aattacatct actaattctc ctgtagtttc tattggtttg tttgctcttt cttcaactat
    53221 aaatttagct atacgcttag cccagttatc ttctccataa tcttttatta tcttagttaa
    53281 ttcatcttct gtataagtat ttacaacatc atatgcagaa aactcacacc taacatccat
    53341 tctcatatca agtggtgcat cttgcatata agaaaatcct ctatcagctt catcaagttg
    53401 atgagatgaa actcctaaat cagctaatac accatcaatt ttgtaaactc ctattttttc
    53461 taattcctct tttatatttt caaaattgct gtgaacaaac ttcactctac tttcatattc
    53521 actcaatctc tcttttgctg tgcttatagc atttttatct tggtcaaacc ctataaataa
    53581 tcccttatct gacagttttt ttactatttc tttagaatgt cctgctcctc ccatagtaca
    53641 gtcaacatac acgccatctg gctttatatt taaattctct atacactcat ttaaaagaac
    53701 tgatacatga tgaaattcca tagttacctc ccttaaattc actttttaaa ctattcttca
    53761 ctaatattat ttttattttt ctttgataaa taaacatgtg caattatgcc accaataact
    53821 ccaattagac ttataacttg tgccattcta agtggtccta acattaaact atctgttctt
    53881 aatccctcta taaaaaatct tcctattgaa tataaagtta tataagtaac tataacttga
    53941 ccttcatatt ttttattttt tctaaatagt aataaaacta taaatattcc aaaatcccat
    54001 atagactcat ataaaaaagt tggatgtacc tttactccat caaccattat tccccaaggt
    54061 aaattggtag cacctccatg tgcttctcca ttaataaaat ttccccatct tccaatagct
    54121 tgtgctaaag gcattccaag tataactgta tcagccattt ttaagaagtt aatattttta
    54181 atcttagtgt atatataacc agctaatatt ccaccaatta accctccatg tatagcaagt
    54241 ccaccacctc taaaattaaa aatttgagac atattttgag catagtaact ccaattaaat
    54301 acaacataat agattcttgc acagattagt ccaactggaa ttgctattat agctatattt
    54361 aatacatcat cttctttaat tcccactctt tttgcttctt ttattgctat taaagttcct
    54421 aaaatcattc cacatgccat aagtattcca taccacatga tgtctatgcc aaacagtgta
    54481 aatgctactc tatccataac gtcacctctt tgtattaatt gaaaatattt ccaaagcaat
    54541 atgctataat tacaattaat tgtaattata ctaaattatg ataacacatt aatgttaatt
    54601 ctacaaaaaa tattaaaatt tattctacaa ctccctacac tatattatat ctaattacta
    54661 taaaattttt taaactaaat ctctcatgac taaagttaca agtgtacata gatatttttt
    54721 aatcaaaaag attgatgcat atatacatca acctcttcta attcttatct atagctctta
    54781 aatttttacc tcaatttata taaatatatt ttataaattt gttttaccaa aataaacatt
    54841 taaaaacata aactaatact taattagtta ttcttattat gattaagttt ctatttaggc
    54901 tctacaatag aaataacaca atactcttct ttaaaatgct cttttaatct ttcttcaact
    54961 tcctcaaaag taatatcttt aataatatct aaataatcca atagatttat atccttaaaa
    55021 acataagata taaaactatt acctataaag tttatagaat caaagcactt aataaatgac
    55081 cctatttttt tctttttagt tctctcaaac tcttcttttg ataatccttc tttttttgat
    55141 ttttctatat aatcaagaat tatttctttt acttttttag gctctttaga atctccagct
    55201 attatgctaa aagcataatc cacttgagac gaaaatccag ctccaaaatt ttcatttatt
    55261 aaaccttgca tatacaaatc ttcataaagc ttacttcctt ttttaaataa catccctaca
    55321 agtatatcag ttacaatctc tttacgtaaa agttctttcc cttttaaacc aacattgcta
    55381 tctttaaaac ctatgttgaa cataggcata gatataggga atttttctat aacctctttt
    55441 tcttttacac tttctggttc ttctggataa aatctttcta tactcttaga gagtctgtct
    55501 actttgtaat tgtttgactt tttagttaca tctataactt tttctacatc taagtcacca
    55561 acaacaaata aagccatatt tcctggatta tagaaagtat tataacattt atatagctct
    55621 tccttagtta tcttatatat actatcaaca gtacctgcta tatcaattct agcaggataa
    55681 tttacataca ttgccttcaa gcaattaaag tatacattcc aatcagggtc atcattgtac
    55741 atttttatct cttgtgctat tatacctttt tccttttcaa cattttcatc tgtaaaataa
    55801 ggtgtttgaa catagtcaat tagatgttct aaactctcat aaaaattttc agtagcagaa
    55861 aataagtagg ctgtcattgt aaaattagta aatgcatttg catttactcc taattttgaa
    55921 aatttatcaa atgcatctcc tccatcaggt tgctcaaaca tcttatgctc tagaaaatgt
    55981 gcaatacctt cattcactct aattttctta tcttctccaa ttggtacaaa ttctaagtca
    56041 tttgaaccat aattagtagc caaaatagca tactttttca taaaacccct tttaggcata
    56101 aaatatacat ctaatccatt ttgcaatttc tcataataaa cttcttcttt caatatatca
    56161 ttaactattt tttccataga catctcctcc attaatttct caagaagtat acagtatcaa
    56221 gctctatatt ttttatagct tcaactatat ctttttttgt aaccttttct atactagata
    56281 ttatgtcttg tacttcagaa tttgtctttg ccatagactg agagaaaaca aaatcagaca
    56341 taccacctat attatcagtt atagatttca tagaattaac taaagcaagt ttactgtttt
    56401 ctaattcttc atcagatata tcacctgctt ttacactttc tacttgtttt tttatcaatt
    56461 ctacagcttt atcataatct ttagtttcta taccagatga tataaacatg atacttttat
    56521 acttctctat tgaagagaat atatagtaac ataaactttc tttttctctt acattaacaa
    56581 ataatttaga gtgtggacca ccacctaaga cattgctccc aactactaaa gcataatatt
    56641 tatctatgtc tgcataatcc acatttgctc tataacccat aactaactta ccttgagtta
    56701 tttccatttg ttcatctata acttttacct catccacatt ttttataaaa tctgctcttg
    56761 gtatatttat aatttcttct ctttcaaatt ttagattttt acttattata tctacaactt
    56821 tatcttcatc aaaattacct tctacaacta tatctatagg tgatgtttta agtatgtttt
    56881 tataatgttc atataactct cttgaagtta tactatctat ttcatcttca taaccaaatt
    56941 cacttacact atatctctct cctttacaca tagcctcaat acatttatct tgcgcatatt
    57001 cctttttatc atttattatt gattgtatct tttctcttaa attctctttt tcaatattta
    57061 aatattcttc tttaaaccca ccatctacaa caagcgtatt atttattact tcattaaaaa
    57121 attctactac ttcttcaaat atactctcat ctaaatactt ttcacttatg ttaattactt
    57181 taaaactcaa tacttgacgt tctccacgtt ttacagcatc agctcccata gatgaaccat
    57241 ataaatcatc taatttattg gatattgctc tagcactagg atatttagcg cttccacttg
    57301 ctattattga tggtattaga gcattttttg tagtttcttt ttcatccaat agtctctgaa
    57361 catataaact tataagattc gatttaaact tttctgtttt tatcaaagta agattaatat
    57421 tattacctaa atttattatt tttttagtat taaccatatg tacctcctat ttttatttaa
    57481 acatcaattt taaaattgaa atttatttat tttttaatta catatactca catataaaaa
    57541 ccttctattt aattatatcc tttttattat tttctaaaag caatattttt atttaaatta
    57601 aatacaaata ttatttaaat caatatgctt taaagtctag tttacaagct tgatatatat
    57661 gatgtataat caatatgtat gtctataatt aaaacaattt tgcaatccta tagattgatt
    57721 gtttaaagtt atataaagaa tataaaaaag aataagagag aggacgatta atatgaaatt
    57781 aggtatagtt ggtttaccaa atgtaggtaa aagcacacta tttaacgcaa taactcaagc
    57841 aggtgcagaa tctgcaaatt accctttttg tacaatagac ccaaatgtgg gagttgtatc
    57901 tgttccagat gaaagattaa acaaactaca agaattatat aattctgaaa aaatagttcc
    57961 tactgctata gaattttgtg atatagcagg tttagttaga ggagcttcta aaggagaagg
    58021 tttaggaaat aaattcttat cacatataag agaagttgat gcaatagttc atgttgttag
    58081 atgttttgag gatgaaaatg ttgtacatgt agatggttct gtagacccac ttcgtgatat
    58141 agaaactata aacttagaat taattttctc agatatagag attcttgaaa gaagaattaa
    58201 taaaactcaa aaagctgcta aggctgataa aactttaggt tctgaacttg atttattaaa
    58261 atcaataatg tctactttag aagaaagtaa atgtgtaaga actatggaat ttacagaaga
    58321 tgaacaaaca ttcgtaaatt cattggattt acttacatct aagcctgtaa tatatgcttc
    58381 aaatgtatca gaagatgatt tagctgataa tggagaaaat aataaatatg ttcaacaagt
    58441 taaagcattt gcagaaacag aagatgccga agttgttgta gtatgtgcac aaatagaagc
    58501 agaaatttct gaacttgatt ctgctgaaga aaagaaagag tttttagaaa cattaggtct
    58561 tgaacaatca gggcttgata agcttataaa atcttcttat gctttacttg gtttaatttc
    58621 attcttaact gctggtccaa aagaagttag agcatggact ataaaagttg gaagtaaagc
    58681 tcctcaggca ggaggaaaaa tacattctga tatagaaaga ggatttatca gagcagaaac
    58741 aatagccttt aatgatttgg ttgagcatgg tactatggct gctgctaaag aaaaagggtt
    58801 ggttaaatta gaaggtaaag aatacatagt taaagatgga gatgtaatat tatttagatt
    58861 taatgtttaa aatgattaat cgtatacact ttgactatta tatataataa
//
LOCUS       DAMJVK010000011        58601 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.011, whole genome shotgun sequence.
ACCESSION   DAMJVK010000011 DAMJVK010000000
VERSION     DAMJVK010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 58601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58601
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.011"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            143..493
                     /locus_tag="PC414_000709"
     CDS             143..493
                     /locus_tag="PC414_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDF2463121.1"
                     /translation="MSAKVTNQYGNIEIDNQVIAQVVYRAAMESYGLVGLAYKSKGIV
                     ELLKGENATKGVRVEEVEEDAIAIELYVIIQYGTNISTVANNIIDRVKYVVEKMTGVR
                     VTRIDINVQGIRVK"
     gene            512..2155
                     /locus_tag="PC414_000710"
     CDS             512..2155
                     /locus_tag="PC414_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HDF2463122.1"
                     /translation="MIQYIDGKRLREMFISGANNLQNNKELVDKLNVFPVPDGDTGTN
                     MSLTISYALKELAKVENDNISDIGKALSKGSLMGARGNSGVILSQIIRGIAKSIEGKS
                     KLSTEDLAKAFKNGSDTAYKAVIKPIEGTILTVVRESGEFAIKTAKKEKDVVKFLSML
                     VKESNSSLERTPDLLKNLKEAGVVDSGGKGLVLIYEGMLASIKGDNIEIKNVDLDTNI
                     STSMDFAKSTTSTDDIKYCYCTEFILESSKVEDTKIRDIMMAYGDSLAVVGDDGVIKV
                     HVHTNDPGNVLQEALKYGQLLTIKIENMKLQHENTLLDVEEKKENDSEPLEEEKEFGF
                     IATSMGEGLANIFKDFGVDHIIEGGQTMNPSTEDFMNAIKHINAKNIFIFPNNSNIIM
                     AANQAKELSDKNIIVIPTKNTPQGFAALVTFNGELSEDENKEAMMNALNSVKSGQVTF
                     AVRDTVMNEIDVKEGNIIGIAEGNLLSAGDYVDEVTSNLIEKLVDEDTAIITLFFGED
                     VTESQANELRTSLEEKFEDVDVELYYGGQPLYYYLISVE"
     gene            2236..4293
                     /gene="recG"
                     /locus_tag="PC414_000711"
     CDS             2236..4293
                     /gene="recG"
                     /locus_tag="PC414_000711"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HDF2463123.1"
                     /translation="MDLYKDVQYVKGIGPKKADKLNKLGIFTLKDLLYYFPRQFEDRN
                     NLKKIAQLEDGEKVTIKAVISSINTFSPKEGMTLTKIDVKDETGSAKLVFFNKSYIKN
                     TFRPGDSILVFGKVKKKFNNLELTSCELEYLTNSPKNTCRFMPVYQLTYGVTNKEIMS
                     IIRTVLEDKELIIQEYMPQRIIEKYRLCSIDFAVRNIHSPSSKESLKIALYRIVFEEL
                     LILQLGLFVFKSGRNKEDGIKFETSKDLKKIISALPFKLTKAQNRALDEIIQDMNLEK
                     IMNRLVQGDVGSGKTVVALLALANCVLNGYQGALMAPTEILAGQHYISLTESLKDFGI
                     NVGLLIGSLTKKQKDTVLEQIKNNEIDILIGTHALIEDKVEFNNIGLVITDEQHRFGV
                     MQRSKLSLKGANPDILVMTATPIPRTLALILYGDLDISIIDELPPGRQPIETIAIEKS
                     KRDRAYNNLVRREVESGRQVYIVCPLVEESEAIEAKSAVELVEELRAEYFHDLRLGLL
                     HGKMKSSEKDEVMRLFKDKEIDILVSTTVIEVGVNVPNATLMIIENAERFGLAQLHQL
                     RGRVGRGSHKSYCVLIYDSKTDVCRQRMAIMEETNDGFKISEKDLEIRGPGEFFGTRQ
                     HGLPELKVANLFKHIKILKLAQQEARYILGEDNNLQLKENMALKKEIIDKFKDTLKEI
                     SLN"
     gene            4352..4906
                     /gene="rsmD"
                     /locus_tag="PC414_000712"
     CDS             4352..4906
                     /gene="rsmD"
                     /locus_tag="PC414_000712"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HDF2463124.1"
                     /translation="MRVISGKARGLKLNTPKNEDVRPTTDRVKESLFNMINSYIMESE
                     VLDLFAGTGSLGIECLSRGAKSCTFVDISKESIDIVKSNIKKARVESESIILNLDFKT
                     AIDKLKLQNSKFDIIFMDPPYYKNMFIEAIEKIDNSNLLNEDGIIVVEHDTNDLFPDK
                     ICKLEKTRDKKYGNTTLTFYKMEA"
     gene            4908..5405
                     /gene="coaD"
                     /locus_tag="PC414_000713"
     CDS             4908..5405
                     /gene="coaD"
                     /locus_tag="PC414_000713"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HDF2463125.1"
                     /translation="MENKPRKAIFAGSFDPITNGHLDIICRASKLFDELQIGVLNNPN
                     KKGLFSFDERVKLIEKSTSHLNNIKVVTFDGLLINYCQENGIGALVRGVRSGADVDYE
                     LQMAHMNRELNPDIETIILPSCTKYSFISSSLIKEVLLFDADIKNLVPKIVLEELKKK
                     TSGGN"
     gene            5409..5933
                     /locus_tag="PC414_000714"
     CDS             5409..5933
                     /locus_tag="PC414_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463126.1"
                     /translation="MKIDLEMIELFEQLEEIFRSASTIPFSHKCTVDKDEVLAIINDI
                     RTLIPEEVTQAVWINKERNKIISQAKQDATNIVEQAKKEAERIQQEYQDNIEELKKNS
                     EDVVKAYVESSEPVVQADQRAKDIVDRAERIANEIRIGSIEYAEDVLSSVEYNLREIL
                     EEVKRDKAELRPKK"
     gene            6277..8196
                     /locus_tag="PC414_000715"
     CDS             6277..8196
                     /locus_tag="PC414_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2463127.1"
                     /translation="MKERAKKILDFIIKNNNVTSQELQEKFNVSKRTIYYDILAINEQ
                     LGKSGNIKNVKHKFIFEGNLCDARKIISTEEDKFLDSDYRKTFILNKILLGEKISIEK
                     LTNEMLLSKNTVVQTITDVKKYLQTMGLRLEYKGKYKIIGDEYVIRELFLIIVQENIL
                     EINSISEEVSSFDTKGHIKLTDYSLLNLTKFVEFLNKRIRDGKTLYSYKYLNEAKKIS
                     YFSNCKELLCEEANENEQAYICTYISSLPSLNSEVKEDVVEEYVDKLIDKFEVNTAIK
                     LESKHEFKKNILRHLHSSYNRIRFKFPIRNPMLDETKYKHESLYKIIKSIIENEEEFP
                     VFEGIREEEIGFIAAYFGGYLRGSRDNGLRRNKVLLVCPNGLMVSKSLEIQLYKYIPT
                     IEIVGIVSIKQLKEVNVYYDYIITTIDIQNVNNVIVVNPLLTSSDVQLLMNKLISVKE
                     NEKYFNLELIIQAIRKNGVINNEEALKADLLNIIHKIDEGEMYQPMLKELINAERVNI
                     IKSVRDWKEAIKIASKPLLEDNSIEELYIENMIKSVEKYGPYIVLADRFALPHASSKE
                     GVNKLAMSLLIVEDEVDLLGKPVNIFMVLAAVDNTTHIRALASLSEMMYEEENVKLII
                     NGDKSSILELINKQN"
     gene            8209..8496
                     /locus_tag="PC414_000716"
     CDS             8209..8496
                     /locus_tag="PC414_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2463128.1"
                     /translation="MKILAVCGFGVGSSMVLKMTIQKALKQLGIECEVENTDINSARG
                     ADCDCIFTSQELLNELKGTCNVDVYPVKKYMDLAEVTEVVQTFLSDKNDKK"
     gene            8629..9888
                     /locus_tag="PC414_000717"
     CDS             8629..9888
                     /locus_tag="PC414_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDF2463129.1"
                     /translation="MLDFISNNILRNPSMLIGLIAAFGLVLQKKKASDVIKGSLLAAF
                     GIIIMETGTGMLVSSIAPINGAFQSISGGAVTKGLSDVTFTASYGGIIGLAMFAGLAI
                     HLMIARFTPIKTIFLTGHMLYWFPFIFVASGVEAGLNGGVLIAFAAVLSALYWSIMPW
                     LLRKYVFDVIGDDSFTLGHPSGCLALISGFVAKLVGNKEKSTEDINIPQSLSFFREIS
                     ISGGMAVFLMFIVVGIVIPGAFKDSNEFIGTYAIKQGLSFGAGLIIMLHGVRMLINQI
                     MPAFQGISEKVVPNALPAFDCPILFNYKPNAVLMGFLVAMLTSTAMILICNYFNVFNL
                     LLIPLVITSFFECGAAAVIGESQGGIRGAIIGTAIAAIFMVVLVGISAVIYSGTIQNW
                     ILIFGGNDLSLFGIISRFIGSLFGGIL"
     gene            9891..10631
                     /locus_tag="PC414_000718"
     CDS             9891..10631
                     /locus_tag="PC414_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDF2463130.1"
                     /translation="MKFKYIDEIRRLIDIIENEEKVNMEKAVQIFVEAIESKNAIFSF
                     GASHAGILTEELFYRAGGCVLINPVFAKSLMLDTAPITHTSKMERLEGYGTALADKTP
                     IKKGDVVIVHSVSGRNPVSIEFAMKAKENKAIVICITNLSYSKSVSSRHSSGKKLYEV
                     CDLVIDNHGQKGDACVEIDGLNQKVSPTSTVIATTVMNSIIAQATQELVNKGLKNPPI
                     FYSANIDGGDELNKKIFDEYKSVIHYEY"
     gene            10954..12264
                     /locus_tag="PC414_000719"
     CDS             10954..12264
                     /locus_tag="PC414_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDF2463131.1"
                     /translation="MKKENKMEGIKVGMLKKLCSLAMVAIMTTTLITGCSSGNGKDSK
                     KDGEKEKLVLWMPPVENNMKEVWDPILDKFEEKNNCEVDLQIIPWDNYSEKFATAISA
                     GEGPDVGYMYAEMYPQFIESGAVEDLTNYATKEDKEQSIYIKTSEMMGGMYGMPFQAA
                     NPGVLYYNKDILEKLGEKPPKTWEDFKRICKKATQDTDGDGKIDQWGLAQGWGAKTFG
                     NLNWNWYPYLWQAGGDIFNDDLKSVKFNDKSGLEAAKFLKELQAYVPEDSLSKDSNEM
                     IESVFGPGKAAFTIMLSSAATSVFDESFPDLNWGFVTGLEDKKAATFGAVDHLSLISS
                     AKNKELAYKLIQHMLSVESMTEFHKAIPRAPITKGEPYQGDERFKEMVENDKNVYRPL
                     VVGPHGVEIYEYLWKELQTMISGDKTPKQALDDAAKYSNDLLAQ"
     gene            12362..13279
                     /locus_tag="PC414_000720"
     CDS             12362..13279
                     /locus_tag="PC414_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDF2463132.1"
                     /translation="MENLVREFEENSKRKKTKLNIKPYMYILPLGIILVAFYVIPIIM
                     SIYFSFTKYNIISPATFIGLENYKKLFTDETLKVSIINTIKFTVVVVPCQTILSLILA
                     VWITGKGNSKIASFAKGAIFIPVLSSMVLIGMVWRALLNGEGSIIYQVLGAFGIESSK
                     LLGDSKTALPTLMFISMWKNIGYFMVIYISAIMNLPKHCYEVAKVDGATKLQEFIKIT
                     VPLLKPTTIMVVFLGSIWSLQVFDLIYTVTGGGPGISTMSIVMHAFNLNFKNFNSGYA
                     MTVANVLFLLIAVVSILQNKLVKRDNSDF"
     gene            13295..14104
                     /locus_tag="PC414_000721"
     CDS             13295..14104
                     /locus_tag="PC414_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDF2463133.1"
                     /translation="MKTARRLLSDGLLLFIACASLVPFIYMLIISLKITYNSYSLDIS
                     FSTVTLQNYIDIFTKKGFAQYFFNTAIVSFSGVLLNLVFSTLAGYSFAKMDFKGSDKL
                     FLFMIMTLIIPSQVTMIPLYIIMKHLGWINSYLALIMPIPTAFGVFIMRQAILGVPKE
                     LLESAKIDGCSDFRILIQIVLPLIKPALITLAIFTFMGAWNEFMWPLIATTKDAMRTL
                     TVGLSTLKTFQITNYGQMMAGATITFLPPFIFYLILQSKFVEGVSLSGIKG"
     gene            14162..15910
                     /locus_tag="PC414_000722"
     CDS             14162..15910
                     /locus_tag="PC414_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HDF2463134.1"
                     /translation="MEISYVLKDCTFKHNKYSVVEAETWKDKDLDIVVAKGEKFAFQI
                     MLKATEEFNCTIDKSSAISWKGLGNRVRLALNMPNSLENNFSINILGYVQDDTKAFVN
                     DAILRDKDVLVEQYLPQTFWIEGQVPEDFEENNLDIGIDIFKSFGYEDEEKVCTIDVP
                     VKVRNVVLKPLDESKFFLDLWQHPSCLARMYKVELWSDIHFEIVDNYLKELASLGEKV
                     ATVIVSDYPWAGQSCYKVYKNPSNLYEYNMVSVSKGLDGKIKCNFESMDRYIGIADKY
                     KMAKEIDLFGLLGNWCAGEFGNPVEGYKDPIRVRYFDEKDKVFKFINNTNDLKEYIGL
                     VLNHLIECGLWDRVRIIADEPNNPEVVKECIEFINSTVGTHQVKYKSATHDQNFLDRA
                     KDEIDDMSINLKLTIQNYKDIESLKKKINDKGGILTWFVCCFPEKPNSFLSSPFVENR
                     IIGWYTYYFGLDGFLRWDYNLWTEDPWKDSSYKFPIWKAGDMFFVYPGKDLKPVRSVR
                     MENLRFGIQDFELFTMLEKEKVREYIEVELMQELLNKKENAEIKGFGDIELGYSLDNW
                     RYDKVKKYVLELLDRV"
     gene            16172..17194
                     /locus_tag="PC414_000723"
     CDS             16172..17194
                     /locus_tag="PC414_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HDF2463135.1"
                     /translation="MFGGINIKRIGIIIMFFLSISFIGFYLVIRDNSDTVKMQRGINI
                     GNALESPKDFPWDVKMSNKFFDDIKDAGFDTVRIPVRFSDYTSDSDNFKIDEEFFKKI
                     DKYVDYALDKDLIVVLDLHHFEEIMKEPRVHKEKFLKIWQQIANRYQKYDKKLVFELL
                     NEPKENLSSQLLNEYIEEAIKIIRKTNPKRTIIVGPYNFYQIDYLNELNIPKDSNIVV
                     SFHYYEPNDFAFQGNIYHKGFEHLSNITWEGTNEQMDYLKKRFDTVENWANKNKVKIF
                     LGEFGITKEAPEVSRRAWIKAVREEAEKRNFSWAYWELASGFGIYNQIEGTWDRDILS
                     ALIEKR"
     gene            17199..19505
                     /locus_tag="PC414_000724"
     CDS             17199..19505
                     /locus_tag="PC414_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDF2463136.1"
                     /translation="MKKNSKKILITLTIITNVIYILWRIFYTVPKEEGKFALICAIIL
                     LFVEIMGMMEMFVHYYGMSNIEYPEKPIISEELYPHVDVFIATYNESVDLVRKTVNGC
                     IHMQYPDKKKVHIYICDDGNREEMRILAEKMGVNYITRTEREGAKAGNLNNAMQHTTS
                     PLIATFDADMIPMHDFLIATVPYFLKNEQAKKDGEKEEYEKVGFVQTPQSFYNPDLFQ
                     FNLHSEGRIPNEQDYFYRDIQLARNRTNSVIYGGSNTVISREALEEVDGFYTYSITED
                     FATGILIQSKGYRCYAIPEVHASGLSPTDLKSLIKQRERWARGCIQTGRRLNILFRRG
                     LGFWQKISYISSITYWYASIKRFVYIMAPILFSVFNVIVVKCTLLQVLVFWLPMYILS
                     SLSLKIFSQNIRNTRWTNIYETIMFQSLMPAVILETFAISKNKFSVTNKSKLEENRMY
                     KFLQGIPYFIYMVLSIIGILKMFVAIFKMSSMTYSVVLFWLIGNLFNLVMATLFISGR
                     QQLRKSERYIAEIDFKLKQNSYVLSSKTIDISENGFAFLLENPEYISPEEEFEVEFRE
                     KSGNEMYIANMKAKIVNVVEVNSKWKYAAYITHIEDSEIDNWMCIVHDRIPTLPMTIS
                     NQLGFFDDLQINVKKRIEKTRTLSRRSPRINMNFQMDIKNTGKLRIVNFNYQYVLLNF
                     ENKSIYPKEIALEINENIVLECDLCEGKIDERGILYKVNNIDSIMQNFFLRDEMMDWI
                     LQNKTILVSKPSEKKEKSIDEFEPMEYI"
     gene            19477..20592
                     /locus_tag="PC414_000725"
     CDS             19477..20592
                     /locus_tag="PC414_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463137.1"
                     /translation="MSLNPWNIFRRDEMKTLKVFFCSTKYLILVFIFFINTTFISSAT
                     SDKILDLSFKKIETDLNSKITYEDTGVKIETDSSKSDKERYLYIYQNIKENWSMYNNF
                     YIEIQNKNKSSQRINLSIQSKNMLEFRLKEGSEVFLEGKNIIYSDKIKEGCIEVPGEF
                     EGKIYVNFNSLINEESNVVLDSNMLSNIVSWGITFIPSDEEHNIVIIKKISLLSEEKL
                     RFLNNIKIIGDEEVQIPVLGQSISQYEVLGLKSDSKIKYSLMGKQDNVSISQKGKLTL
                     SNKAKPGQIILQVNVDDKFKIGKKITLTESWSVNKKDKDGVPYTLVYPEQSPTVQDMK
                     KINFMNNIITFVRILFVSLVIICFGIYLYWKKCSKTK"
     gene            20610..22028
                     /locus_tag="PC414_000726"
     CDS             20610..22028
                     /locus_tag="PC414_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HDF2463138.1"
                     /translation="MLFSSLIFLFYFLPITLVLYYVFRFNRTIQNMILLAVSLFFYAW
                     GEPKFVVIMIVSIIMNYIFGLLVDRYRESKIKVKVFLVLMCAYNIGVLFIFKYLAFAL
                     RNISELISTELTIPSIVLPIGISFFTFQGMSYVIDVYRRHGEVQKNPFYVGLYIAFFP
                     QLIAGPIVRYESVAEQILNRKETWNKFSIGTCRFITGLGKKVLISNNMAIVADYIYTM
                     NSQGEIAASLAWLGSIAYTLQIFFDFSAYSDMAIGLGLMFGFKFEENFNYPYISKSIS
                     EFWRRWHISLGMWFKSYIYFPLGGSRVANKDIMIRNMFIVWLFTGIWHGAEWTFVIWG
                     ILNFVFLIIERFIMFEKIENHNFIKHIYALLVVNFGWVLFRSPNLKEAYNYFKAMFGG
                     NGAIWSDYTYMFLKEYWVFFIFAFIFSVPIAKKINKFVVEQAKYSMIFNVFYPISAIL
                     LFFISVTYLVTGSYNPFIYFNF"
     gene            22043..23251
                     /locus_tag="PC414_000727"
     CDS             22043..23251
                     /locus_tag="PC414_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463139.1"
                     /translation="MNRFFKVRLITAIMFLIILFAFSIFNVCYSGKDTIQEIKNSIAE
                     KSSLKDTISSIDNTVNDKVIYKNTFIETYGYLQKLMGKNEFSKFSVVKDTSGKMHYTY
                     FAKAPNPVGVIADRVRKFSDEMEKQNTKLVYLMTPDKYIKGVTKFPKGIPYSYSNETA
                     DNFLNQLKKYNVDYIDFRENILKSGIPKDDLFFKTDHHWKVETSFWAFGELVEQLNKK
                     YNMNLDENHYYRDKDNYNSIVYPKSFLGSMGRKTGILYGGIDDFTLIYPKFKTNYTYY
                     TDSKSQKFELKGRFEESLILSYPFNADLDLMDGQSDKYFTYLLGNRPLVKIKNIENPD
                     GLKVLFVKDSLIVPTAAFFSSVCSSIDMIDPRYYDGDILEYAKSHDYDFVFISVYPQN
                     LTKEFFPFCE"
     gene            complement(23325..24713)
                     /locus_tag="PC414_000728"
     CDS             complement(23325..24713)
                     /locus_tag="PC414_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HDF2463140.1"
                     /translation="MESSFLSQFLMITDFKTIFFIILLLGTFFIVRQFEKKKIKFSLR
                     TIYATVLGLILGIIIQAFAGFPNDTTQVIWLQEVSKWYSLFGSGFMDLLKMLVVPLVF
                     LSIIKVIINMKDNNLGTLTFKSLGMLLATTAIAAIVGIIVANIMKLGVGANMPTLVEN
                     QELKEINSLVDTLRGLLPSNPVMAMANGNIVATIIFATFIATSIKRLSKKHFETIKPF
                     IGFIEASYKIIVSVSMTVIKFMPYAVVALLANTITSQGISSIVSVLQFILALYISVAI
                     LFIIHLIFITLNGLNPITYIKNSTEALLLAFTSRSSLGTLPVTIESLVQNHGVDEGVS
                     SFTASLGANMGMNGCAGIYPALMAVTLANMAGVNMDMSFYVMLLIVITISSFGIAGLP
                     GTATMAVSVVISGVGLGSYFPLVGGILAIDPILDMGRTMINVNGAMVSSITVGKSLGK
                     IDKEVFSKKNIS"
     gene            25054..26388
                     /locus_tag="PC414_000729"
     CDS             25054..26388
                     /locus_tag="PC414_000729"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HDF2463141.1"
                     /translation="MRVIIIGGVAAGMSAAAKLKRIKPEYEVVVYEKTEIVSFGACGL
                     PYFVGGFFNDADELLARTPEKLREAGIDLNIFREVVEVDSESKKIKVKNIKTDEIYED
                     YYDKLMIATGARSIMPPIKNIKLKNVSTLKSLYDGEYLKKLLSNEDNKRVTIIGAGFI
                     GLEAVEACKKLGKDVDVIQLEDRILPQVFDKEMTDVLEEEINRHNVNLHLDEMVVELC
                     GEDKVEKVITNKGEIDTDVVIIATGVRPNTEFLSNTNIKMLKNGAIVVDEYGRTSVED
                     IYSAGDCATIKNIVSNENVYVPLATGANKLGRIVGENLAGREISYQGSLSSSCIKIMD
                     MEAASTGITERQAKDLGINVKSKFISDYNQTHYYPGRNKIYVKLIYDADTKVILGGQV
                     AGFKDAVQRANVLAAAIFGKMTTNQLGMLDLCYAPPFARTWDVLNVAGNVSK"
     gene            complement(26530..27447)
                     /locus_tag="PC414_000730"
     CDS             complement(26530..27447)
                     /locus_tag="PC414_000730"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HDF2463142.1"
                     /translation="MIDLTLLGCGGNVPMPNRFLSSVFINYKGRKILIDCGEGTQVSM
                     KLKKCGFKDIDLICITHLHGDHIFGLLGLLSTIGNSGRTSDLTIVGPVGIVDCIRSMR
                     NLVEYVPYTLKIIENPQGNFSLDNKVLRNLEISTISLEHSIECLGYSFNFRRNPKFDI
                     DKATKNEVPKILWNKLQEGQNIVLDSKQYTPDMVLGELRKGVKISLTTDTRPIESIPD
                     FIKDSDLFICEAMYGDDLDISKAVRNKHMTFREAANLAKLGNVKQLLLTHFSPSLDIP
                     SMYLENATNVFENTILGEDRLSLRLNFDE"
     gene            27655..28539
                     /locus_tag="PC414_000731"
     CDS             27655..28539
                     /locus_tag="PC414_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HDF2463143.1"
                     /translation="MKVLKKITILSLITLTLVGCSNGEEKKVVEGSKKTQNSKKDEQP
                     IIVHKDANILKDRYEPPKGFKRVDVEKGSFESFLRNTKLKNYGEKVKYYDGRTKESKG
                     IYDSVFDVDIGNRDLHQCADAIMLMRAEYLYQNKLYDEISFDFVDGFKAKYSKWAQGY
                     RISVKDSGSSWYKATEYSTSYESFRKFMDIVFAYSGTLSLEKELEPVKVEDMKIGDVF
                     IVGGSPGHASIIMDMAENEKTGEKVFMIAQSYMPAQQTQLLINRKNPNLSPWYSLDFE
                     GDLTIPQWTFKRDQLKRF"
     gene            28752..30593
                     /locus_tag="PC414_000732"
     CDS             28752..30593
                     /locus_tag="PC414_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HDF2463144.1"
                     /translation="MKIRRYLILVIIILITTSQSFISYGLERFEEITIFHTNDIHGRY
                     AEGDDHIQIGNLATLKKETPNSILVDAGDCLHGLPIVNMDRGKSAIELIKCAGYDYIT
                     PGNHDFNYGKDRLFELSKSANLGENTLKFLSSNIFENGKKVFDSNDIKEIDGIKIGFF
                     GLSTQETKSKTGFKNVEGLEFKNPIESATEQVNELKGKGANVIVAICHIGTNSLSKPN
                     SIDIANEVQGIDLIIDGHSHTKFENGKQVGDTLIVSAGQYLENIGEIKLTLDTNDVED
                     IKIENKSARLIGKEEALKYKLDSVVSKKVDKIKEQQEKILDKVIGVTTNTLDGSYENV
                     RTKETNLGNLISDILLDKTKADISLFNGGNIRDTIEKGNITRRDIVDVFPFSNTIVTK
                     ELTGAQIKDVLEHGVKLYPEKSSAFLQVGGISYYFNPKQKEGEKITNIQKEGKPLDLN
                     KKYVVATNDYIASGGDEFPCFSKEPVLKEFGNLESAVIEYIEYKKEISKNVDGRIGIK
                     SKDELISKNHESDLILSENEKNINDNEENLSNNIKSSDSDKNKSLDKTSSRESSNLGI
                     SKREAKEKSPKTGDLGFSNSIIIFIVSSTLICLLNFNQKELKDKKSK"
     gene            30898..31605
                     /locus_tag="PC414_000733"
     CDS             30898..31605
                     /locus_tag="PC414_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2463145.1"
                     /translation="MNNILLLEDDKSLNRGISFKLKKEGYNVFSAFGIEEAKNIFAKE
                     EICLIISDIGLPDGSGFDFCEEIRKKSDVYIIMLTALDEEVDIVTGYDLGADDYITKP
                     FSLMVLISKVNALMKRVNTVKNYTLLVCDDLFFYYIENKLIIRTDDKEEEIILSKTET
                     KLLKYLMENSMQTLTKEQLLESLWDSSGNFVDDNTIAVNIRRLRQKVEKNPSEPKYIK
                     TVRGVGYMWGERSIKKC"
     gene            31599..32909
                     /locus_tag="PC414_000734"
     CDS             31599..32909
                     /locus_tag="PC414_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2463146.1"
                     /translation="MLKNMNLKDGLSVKDDYIMRRVIVYSVLSVLIVTLVFILFEYSR
                     LHKLYEHQIKNTQNVAGILIEKYPNDEIDIVNSIYNSDYSKVSTGEKAFKKFGYGLEN
                     KMSNDKNFSLYLKSFFKESFFIFLMMFIVVIGIIYYFIRYINKRLSKIYFIVDKMSQE
                     NYLKDTTESEETGYLVEYNEYFKEGIFSKINNCLYELNRSLKIKFIKLEKEKESVKSL
                     VTDISHQLKTPLASLKLYNTLLIEEELEEDEANEFLLTNKASINKLENLINSLVNISR
                     LEISMINIKKEDNDIKNTILNAVKSVTPKAKSKSITLNLNEFNSRIIPHDKKWTEESI
                     FNVLDNAVKYTQVNGEINISVEETANFFKIIIEDNGIGIEKSEFNNIFKRFYRGSADE
                     IESVEGSGVGLYLSRKILEEQGGNIIVSSKVGIGSKFSLFLTTM"
     gene            33026..33709
                     /locus_tag="PC414_000735"
     CDS             33026..33709
                     /locus_tag="PC414_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2463147.1"
                     /translation="MKILYTENLSKHYGKGESLVRALDNVDLEINEGEFVAIIGKSGS
                     GKSTLLHMIGGLDIPTSGKVYIDNKNIFTLKEEELAVFRRRKIGFIFQSYNLIPSLNV
                     WENVVLPIGLDGREVDEPFIKELLKSLGLENKHDVLPNTLSGGQQQRVAIARALATRP
                     AIILADEPTGNLDSKTSDEVMSILKSMSKKYSQTLVMITHDDSIAQMADRVIFIEDGR
                     VSKVGDKND"
     gene            33702..36089
                     /locus_tag="PC414_000736"
     CDS             33702..36089
                     /locus_tag="PC414_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2463148.1"
                     /translation="MINTTKIAKSNLKQNKSKSILIIITIILSTLMLSSIGIYIVDAG
                     AYQKENTIKYSGNYQGILANVDEKQADILSNHADVELTGKMNGVGVEKLEDDSNISLA
                     YMNEDALKLNSFEFIKGKLPSKENEIVLDSGALKALGYKNKDLGEKIKISYNDYKSDK
                     KIEKEFIISGILKTSEISEAGKYYYAIISESYMRNTRDMSQEDFNIYVKLNDKSNLSI
                     EQAKSKLNKIANDVGLDTINVAVNENYINALKPDMETIMGGVFVGLVIVLSSILVIYN
                     IFYISIVTKVQEFGKLRAIGATKKQVKNIVFKEGFILAGIAIPIGIILGYVLANIIIK
                     SFMDIDVKSSRLPVILSVVVISFISVVLSLLKPMKVASKVSIVDAVRYTGNKISNKSK
                     RKGYKNINLKRLSHANLERNKKRTYMTLASLILSGTIFITVSTALESFDAEKMAREHF
                     PYDIEVRLSGYEMNSDKNPKDNLNILQMDNPLSKDFFNQIKNMEGIKRIESARSVKIG
                     MEDYDVEFKYDLLQSINENDVKSLNKNIIDGKINLERLQTGDEIVITHVDTAKEMGVK
                     AGDKIRLTLYDGDKKIKKEFKVQAIAMGVPSFGIGKDFIDRTLKYDSTSSLGIYTEEG
                     KYQEIKDSIKKIAKNNGFLETDFIDSRIESNKATISFIKIMGYTLTGIIGVIGFMNLV
                     NTMITSIVTRKKELGMLQAIGLTNKQLVKMLNSEAMYYTSGMMIGSILFGGILGYIAV
                     IFLKKTGLSYATYSLPIVPILLMIVCILIAQFITTYLIGRSFNKESLIDRVRYSE"
     gene            36369..37511
                     /locus_tag="PC414_000737"
     CDS             36369..37511
                     /locus_tag="PC414_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HDF2463149.1"
                     /translation="MNKKINYAPGPTETRENVRLARAEKTTNPDIDIDFVEFYKKTCE
                     KFGNIIGTKNDVYILSGEGILGLEAACASLTEKGDRVLVIDNGIYGEGFKDFVTMYGG
                     EYVLFSSEYTKSIDIDELKAFLDEDSNFKYATVVHCDTPTGVLNDVSKICPLLKEYGI
                     LTVVDSVAGMVGERLSVDESKIDIILGGSQKAISAPAGLTIVGISQDAKNCIKNRKTD
                     VIGFYCNLNIWEGYYEKKYFPYTMPISDIMGLDKALDNILEEGIENVLNRHEKIAYSV
                     RKSIEEYGLELFLEEGYSNTVTAIKIPESIGALELTDYMLKNYNTLVATSLNQYMDTI
                     LRIGHMGENANLNKIEHVLNVLDKSLSALGFKGNGNLLNLFNKYYF"
     gene            37530..38054
                     /locus_tag="PC414_000738"
     CDS             37530..38054
                     /locus_tag="PC414_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDF2463150.1"
                     /translation="MEVTAKQKFTTKDMVETSLLIALVFIATKFINIRLPISINGGLV
                     HLGTAMLFISAIVFGSKKGALSGAIGMSLFDLISGWTLWAPFTFIVRGVMGYLLGKIA
                     WANGNNGNNFLINVIGICVSSIWMLFGYYVTEVILYGNFIVPLTSIPGNLMQVLIGLI
                     IALPISKVLKKCIK"
     gene            38154..38972
                     /locus_tag="PC414_000739"
     CDS             38154..38972
                     /locus_tag="PC414_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2463151.1"
                     /translation="MLSVTRYSIKNVRLKPWVKFIWYFETKSNILLNNKLLPTDSIDI
                     ILNLSDVMEYKIENQDYIASNIHFNGIRDKHGFIIQHGNIRVIGISFYPFGLYPFLKI
                     PISEFNRQIVDLEAVSKLFAKKLEESLNPTKAIEKVVLCLEEVLLSILEEDLISNKYV
                     KLLNSFIYVNRYSNIKAFCDDTNINIKTLERICLKYTGYTPKILKRIYRFKMASNQLI
                     YNYKDDELFDFIYENEYYDQAHFIKEFKKFSGTSPIKFSGENKTIKENIKYSYL"
     gene            39050..39361
                     /locus_tag="PC414_000740"
     CDS             39050..39361
                     /locus_tag="PC414_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HDF2463152.1"
                     /translation="MKIITEIVEFNVEENLSKEEFIEIVNDLELKFHSIQSGFIDTEL
                     LYDEKSQKWIMIQHWESIEDLKSVSSKMFKDSSTEKFRKALKNQTVKMNIIPQIKSWK
                     L"
     gene            complement(39538..40221)
                     /locus_tag="PC414_000741"
     CDS             complement(39538..40221)
                     /locus_tag="PC414_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463153.1"
                     /translation="MQKEVAMNKLIKDCVNFFNDCGFSYSICGGYALELFANMHIRPH
                     SDIDIWISDTDKEKSIQYMLNQGWDIYEPLGNYLLRPIIDLNKQHIEQICVFAIKPDC
                     SFIELKKVEDDNYKMNIQCDEQLQFDFIELIYNPQKDGKFLFSQNQRIIRDMDKARLI
                     TSDNIPYMAPEVVLFCKSIYIDRLGYQKDFDVTVPLLSQEQRDWLVNALITAYPDGHA
                     WIDQLANQY"
     gene            complement(40314..40955)
                     /locus_tag="PC414_000742"
     CDS             complement(40314..40955)
                     /locus_tag="PC414_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2463154.1"
                     /translation="MGRKKEPDNTIQKILDISHKLFLEKGYEQTTIQDIVDNLDGLSR
                     GAIYHHFKSKDEIIKAITSRLYNDGFYGYEVKNDEGKTALEKLQYRLLHTYEQHNKQD
                     IFLMSSSLLRNPKYLATQFDEIINDIVPWIKALIEEGNQDNSMEVKNPEIAAQMFGFL
                     LNTWCIPTVYPGNPQQMIERFTLFGEQAAFMGISFMNDKIMCEYQKFVNKFSK"
     gene            complement(41129..43132)
                     /locus_tag="PC414_000743"
     CDS             complement(41129..43132)
                     /locus_tag="PC414_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HDF2463155.1"
                     /translation="MKSQKRFKILDIDVYLQPFESDIKKRMKHYEDMKSKTLKNYKDF
                     SSFANAHLYYGFHQTNDGFFYREWAPNADSLSLIGDFNNWNRKSHPLRKIGSGNWEIF
                     IPGQNSLPHKSEVKVQVTANGKTFDRIPLYIKRVIQKDFGGFNGQIWQPKTPFIWTDN
                     DFDLKNITSPLIYECHIGMSTESESIGTYNEFTEKILPKIKKAGYNTIQLMAIMEHPY
                     YASFGYQVSNFYAISSRFGTPEDLKNLINTAHSMGIAVLLDLVHSHAVKNTLEGINEF
                     DGSEHQFFHSGSKGNHPAWGTKLFNYGKPEVIHFLLSNIKFWLNEYHFDGFRFDGVTS
                     MLYHNHGLGVSFDSYEKYFSMNTDIEAITYLQFANELMKEIKPNSISIAEDMSGMPGM
                     CIPIKDGGIGFDYRLAMGVPDFWIKTISNLSDEDWDLGKMWYELTTRRPGEKNIGYCE
                     SHDQALVGDKTIIFWLADKEMYWNMEINSNNHVINRAISLIKLIKLITFSLAGEGYLN
                     FMGNEFGHPEWIDFPREGNNWSYKYARRQWSLSEDNNLKYKQLLNFDKSMLELTKYSD
                     IFIQNSHELIHMDNDKKILVYSKGKYLFIFNFHPNLIQSVDISKFQNTNYKTILNTDM
                     VEFGGNTKKENLRDYDVYFSSTPPSSKMPIASRTAIVLLDNGI"
     gene            complement(43285..43923)
                     /gene="recX"
                     /locus_tag="PC414_000744"
     CDS             complement(43285..43923)
                     /gene="recX"
                     /locus_tag="PC414_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HDF2463156.1"
                     /translation="MGIITKIEQQKRNDDRVNIYVDDKFFIAIFKELVYTFNLKKGNE
                     INEEELKPILDDEMYIKAKNKALNILSHADQSEKKLKEKLSSEFDENIIERVIDFLKS
                     YNLVNDSVLAQKIVNTNVNLNKCGKNRIKQNLYNKGINRSTINEAVSELDKDTEFENA
                     MYLAKKRYERVKKEDKKKIYQKISQHLSYKGFDYDIIKRVLNKLLNFDEYDI"
     gene            44199..44888
                     /locus_tag="PC414_000745"
     CDS             44199..44888
                     /locus_tag="PC414_000745"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HDF2463157.1"
                     /translation="MRSENLVKMAELKNTEKLEKFNRHKGKIKIGILGGTFDPIHYAH
                     LATAEFIRDKYDIDKIIFIPSGNPPHKLCITTDKYDRYNMTLLATESNEDFLVSKVEI
                     ERKKRTYTIDTLKYLKKKYKNADIYFITGADAICSVEEWKDVKKNFELATFIAATRPG
                     ISLLRSQETIEKLTKKYNADIITVYVPSLDISSTYIREQLNEGKSIRYLVPENVENYL
                     YENKLYQYGDD"
     gene            44889..45461
                     /gene="yqeK"
                     /locus_tag="PC414_000746"
     CDS             44889..45461
                     /gene="yqeK"
                     /locus_tag="PC414_000746"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HDF2463158.1"
                     /translation="MNLENINQRLNQMLPVGRLSHSKNVAKCAEKLCEIYGCDKEKAY
                     LAGMIHDCAKYLSDKEIEDYVNKYEIYLDPIEDGNRSLSHSVIGAYICEYEFEVEDED
                     IINAIKYHTTGREDMSLLEKIIYIADLIEEGRKFPVVDTLRELAYGGKLDEALLTSFN
                     NTLMFVINKKEEIHPRTVMARNYLIKEKLL"
     gene            45578..45928
                     /gene="rsfS"
                     /locus_tag="PC414_000747"
     CDS             45578..45928
                     /gene="rsfS"
                     /locus_tag="PC414_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDF2463159.1"
                     /translation="MTVEQMTKIAYDAIEDKLGQDTVIINIGKVSSLCDYFIITTASS
                     QRQVKAIADNVEDELAKLGLEPRGKEGQGTQTWVLLDYGDIMVHVFNEENRGFYNLEK
                     LWKDAPYIDIDTLA"
     gene            46282..48702
                     /locus_tag="PC414_000748"
     CDS             46282..48702
                     /locus_tag="PC414_000748"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HDF2463160.1"
                     /translation="MSVYNFKEVESKWQKIWKDNNQYKMDTAQTEKPNYYTLEMFPYP
                     SGKIHMGHVRNYSIGDVVARFKKMEGYNVLHPMGWDSFGLPAENAAIKHGIHPHKWTM
                     ENIEEMKEQLNLLGISYDWDKEVATSTPEYYRFTQEIFLKFLEHGLAYKKKSYVNWCP
                     SCETVLANEQVVQGACERCKATVLKKDLEQWYFKTTEFAEELLNDLDILDGWPEKVKT
                     MQKNWIGKSTGADLVFDIDGTDKSMTVFTTRPDTTYGVTYMVLAPEHELVKELVAGTE
                     YEADVEAFVQKMHTMTEIERTAADVEKEGMFIGRYVINPLNGKKVPLWIANYVLVEYG
                     TGAIMAVPAHDERDRDFAEKYNLDIIDVITEDNKMINSEEFNGLDASEGFEGIIDKLE
                     KEGRGKRTINYRLRDWLVSRQRYWGCPIPVVYCDECGIVPVKKEDLPVLLPTDVEFTG
                     KGESPLTTSKQFMSTTCPHCGKPARREVDTMDTFVDSSWYFLRYVDSNNENEPFSKEL
                     VNRWHPVDQYIGGVEHAIMHLLYARWFVKAFKSMGMVDFNEPFKNLLTQGMVLMDGSK
                     MSKSKGNTVSPMDIIDEYGADTARLFVLFAAPPERDLDWSEQGVDGCFRFLNRVYRLV
                     DELADVVKKDVEFGELNSQDKDMRYTIHSTLKKVTADLSEKFGFNTAISALMELINDM
                     YKYKELDNINEAVIKEGVQTIVTIIAPFAPHLGEELWTMIGKEGSVFDIDWPKYDEKA
                     LVKDEIEVVVQVNGKVRGKLTVNSNISKDEMEKVALEDEKIKGLVEGKTIVKVVAVPK
                     KLVNIVVK"
     gene            49555..50223
                     /locus_tag="PC414_000749"
     CDS             49555..50223
                     /locus_tag="PC414_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDF2463161.1"
                     /translation="METVTNNQSGVEIQLSLFEAIKDLLLINPIFLLGIILIGIFLVV
                     IFKKNSDIPKVKTSILSLAMYYYLCLTLSHIVGIPTFSEYIRLSQLGETFFNPNINLI
                     PFSDGFSLSFILNILLFVPLGFLCPLISKTFERLRNTFLIGFGLSLFIETIQLFTLHR
                     ATDIDDLLTNVIGTIIGYFCFKLIAKLRIVKLHSDDDSMEQDYTVCIPIAIIIVALIL
                     GFFS"
     gene            50263..50343
                     /locus_tag="PC414_000750"
                     /pseudo
     CDS             50263..50343
                     /locus_tag="PC414_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005541856.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(50706..51823)
                     /locus_tag="PC414_000751"
                     /pseudo
     CDS             complement(50706..51823)
                     /locus_tag="PC414_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861579.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            52039..53409
                     /locus_tag="PC414_000752"
     CDS             52039..53409
                     /locus_tag="PC414_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp29"
                     /protein_id="HDF2463162.1"
                     /translation="MKLKKLLSLGLVSMFVAGFPISANALDKIDKIQGADKYETAGII
                     ADKQSYTTAILINADSTMADGLSASGLAGATNAPILLTKKNNIPNATLKRVEKAKKVY
                     IIGGESSIDKATETFLKDKGIETRRLQGNDRIKTSYNVAKEINSINKVNKVILTNAFK
                     GEPDAMSAAPVAVRDKAAIVLTDGKSVPFNTTGIESYAIGGTSSMSDSLVNDTNSTRL
                     GGVDRYDTNKKIVNKFYNGAKEFYIASGTDLVYALVASPMAKNNPIVLVDIGSNKDIL
                     KNAAKVTGIGNLSDKIMLECENTINNISLPSSNKNIICGLKIDFDSNHIIWQDKGSYL
                     ECYDDNLEYYTEKYKDPTVPINIENTSTQNLDVTKLKVYGEANSTLYMGQYAKIKMDF
                     NSFGVLKPGEKCTIYLDIISLDPSRSMTLTFSYVGDKENYKYEQGLASRILFYNPDGE
                     DFFEYK"
     gene            53703..53861
                     /locus_tag="PC414_000753"
     CDS             53703..53861
                     /locus_tag="PC414_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463163.1"
                     /translation="MDNFLQGILASLSASLIVYIASKLFRKRKKPLKAATKSGWEFDL
                     KIRFHKTK"
     repeat_region   54147..54705
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=54147..54176
                     /rpt_unit_seq="gttttatattaactatatggaatgtaaatt"
     regulatory      complement(54846..54943)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            55170..55553
                     /locus_tag="PC414_000754"
                     /pseudo
     CDS             55170..55553
                     /locus_tag="PC414_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     gene            complement(55731..56726)
                     /locus_tag="PC414_000755"
     CDS             complement(55731..56726)
                     /locus_tag="PC414_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDF2463164.1"
                     /translation="MSIKKKVAIVFTGGTISMTVDDKVGAAIPTLSGEQILSMVTNID
                     KVADVEVFNFDEIPGPHITPYRMMELKNYVNDLLARKDITGVVITHGTDSLEETAYFL
                     DLTIDNIKPVIVTGAMRSSSELGYDGSSNLSASVCTAVSKDAMGKGVLVVLNNEVLLA
                     SEATKTNTLSLNTFKSLTSGPLGIIDCNELVLTRDIVNRTIIDTDKVESKVALFKAYV
                     GENADFIRFAVDSGYKGIVIEAMGRGNIPPQMLSGVEYAREKGLPVVIVSRCHSGRVF
                     DSYGYFGSGRDLKNLGCIFGGDLPGQKARIKLILALGKTDNLDEIKDFFEKGIYC"
     gene            complement(56847..58475)
                     /gene="aspD"
                     /locus_tag="PC414_000756"
     CDS             complement(56847..58475)
                     /gene="aspD"
                     /locus_tag="PC414_000756"
                     /EC_number="4.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 4-decarboxylase"
                     /protein_id="HDF2463165.1"
                     /translation="MKNHTYKEIKNIYGKISPFEFKDKLIDIAKYTAKENNRELLDAG
                     RGNPNWTCSTAREAFFTFGHFAITETRSNWDLGHLAGMPQKKGIKERFFKFINENIDM
                     PGAYLARDIINFGINELGFDGDEFVHELADGIIGDNYPLPDRMLPHMEKIVHDYLVQE
                     MKYDISGKYGDVEIFAVEGATAAMCYIFDSLMANELLKKGDTIALMTPIFTPYLEIPN
                     LPRYDFKVVNINANEVDEKGAHTWQYTKKELEKLRDKSIKALFVVNPNNPASIAMDET
                     SCNNLIDVIENYNKDLMIISDDVYGTFVEDFSSLMSKLPYNTVGVYSYSKYFGVTGWR
                     LGTFALHKKNVFDKKINDLTGELKKSVDKRYSDMSLNPSSLSFMERVVADSRLVALNH
                     TAGLSTPQQVQMAFFSAFALIDKVDAYKKLNMSICHRRQKLLFEALELPINENKNNAA
                     YYTQFDIEEWAKLNYGEDLFKFISKTASPVDVLYKLANDYSVVLLSGNGFYGPEWSIR
                     ISLANLYDEAYTKIGKAIREILNGYIVEWQKIKK"
BASE COUNT        22580 a         6653 c         9936 g        19432 t
ORIGIN      
        1 tttatccaaa aacttttcta ggtttaaatt ataccatatt tatagtatga tatgattata
       61 attttatata ataaataagt aaaaatagta aaaaataaat taagaaatgt ttttaataaa
      121 aataagctta agggggtcaa ccatgagtgc aaaggtaacg aatcaatatg gaaatataga
      181 aatagataat caagtaatag ctcaagtcgt atatagagct gcaatggaaa gttatggctt
      241 agtaggttta gcatataagt ctaaaggaat agtagagcta ttaaaaggcg agaacgcaac
      301 taaaggtgta agagttgagg aagtagaaga agatgccata gcaatcgaac tttatgttat
      361 aatacaatat ggtacaaata tatctactgt tgccaataat ataatagata gagtaaaata
      421 tgtagttgaa aaaatgactg gcgtaagagt tactagaatt gatattaatg tacaaggaat
      481 aagagttaag taatttcata ggaggaaaca aatgattcaa tatatagatg gtaaaaggtt
      541 aagggagatg tttatttcag gtgcaaacaa tctccaaaac aataaagagt tagttgacaa
      601 gttaaatgta ttccctgtac cagatggaga tacaggaact aatatgtcat taacaatatc
      661 ttatgcctta aaagaattag caaaagttga aaatgataat atatcagata tcggaaaagc
      721 attatctaaa ggttctttaa tgggagctag aggaaactca ggagttatac tttcacaaat
      781 aatcagaggt atagctaaat ctatagaagg aaaaagtaaa ttatcaacag aagatttagc
      841 aaaagctttt aaaaatggtt cagacacagc atataaagct gtaataaaac caattgaagg
      901 aactatactt actgtagtta gagaaagtgg agagtttgca ataaaaactg cgaaaaaaga
      961 gaaagatgta gtaaagtttt taagtatgct tgtgaaggaa tcaaattctt cattagaaag
     1021 aactccagac cttctaaaga atctaaaaga agctggtgtg gttgactctg gtggaaaggg
     1081 tcttgtactt atatatgaag gaatgttagc ttctataaaa ggcgataaca tagaaataaa
     1141 aaatgttgac ctagatacta atatttcaac aagtatggat tttgcaaaga gtactactag
     1201 cacagatgat ataaaatatt gctattgtac agaatttatt ttagaaagtt ctaaggtaga
     1261 agatactaaa ataagagaca taatgatggc ttatggtgat agcctagcag tagttggaga
     1321 tgatggagtc ataaaggtac atgtacacac taatgaccca ggaaatgtac ttcaagaagc
     1381 tctaaagtac ggtcaattat taactataaa aatagaaaat atgaagttac aacatgaaaa
     1441 tacattactg gatgtagaag aaaagaaaga aaatgatagt gaacctttag aagaagaaaa
     1501 agagtttggg tttatagcta cttctatggg tgaaggtttg gcaaatatat ttaaagattt
     1561 cggagttgac catataatag aaggtggtca aactatgaat ccaagtactg aagattttat
     1621 gaatgctata aagcatataa atgctaaaaa tatatttatt ttcccaaata acagcaatat
     1681 aataatggct gctaatcagg caaaagaatt gagtgataaa aatataatag ttatacctac
     1741 aaaaaatacg cctcaaggat ttgctgcatt agtaactttt aatggagaat taagcgaaga
     1801 tgaaaataaa gaagctatga tgaatgcttt aaactcagtt aaatcaggtc aagtaacttt
     1861 tgctgttagg gatacagtga tgaatgaaat tgatgttaaa gaaggtaata taattggaat
     1921 tgcagaagga aatttattat cagcaggaga ttatgtggat gaagtaacat caaaccttat
     1981 tgaaaagttg gttgatgaag atactgctat aataacatta ttctttggtg aagatgttac
     2041 agaatctcaa gcaaatgaac tacgtacttc tttagaagaa aagtttgaag atgtagatgt
     2101 agagttatat tatggaggac aacctcttta ttattactta atttcagttg aataatgatt
     2161 ataagaacct gtatgaccaa gtagctttta taaaattggt tgtacaggat ttttgtgtag
     2221 taggtgatat atttgttgga tttatataaa gatgttcaat atgttaaggg aataggacct
     2281 aaaaaagcag ataaattaaa taaactgggt atatttacac taaaagacct attgtattat
     2341 tttccaagac aatttgaaga tagaaataat ctaaagaaaa tagcacaatt agaagatggt
     2401 gagaaagtta cgataaaagc agtaatatca agtataaata catttagccc aaaagaaggt
     2461 atgactttaa caaagattga tgtaaaagat gagacaggct cggctaaatt agtgtttttt
     2521 aataagagtt atataaaaaa tacttttcgt ccaggagatt caatattagt ttttgggaag
     2581 gttaagaaaa aatttaataa cttagaactt acatcatgtg aattagaata tcttactaat
     2641 tcacctaaga atacatgtag atttatgcct gtgtaccagc ttacatacgg tgttacaaat
     2701 aaagaaatta tgagtataat tagaacagta cttgaagata aagaattaat tatacaagaa
     2761 tatatgccac aaagaattat agaaaaatat aggctatgta gtatagattt tgcagttaga
     2821 aacatacatt caccaagtag taaggaatca ttaaaaatag ctttatatag aattgtattt
     2881 gaagaattac ttatactaca actagggttg tttgtattta aaagtggcag aaataaagaa
     2941 gatggaataa agtttgaaac aagtaaagac ttaaaaaaaa taataagtgc tttaccattt
     3001 aaactgacta aagctcaaaa cagggcatta gatgaaatta tacaagatat gaatttagag
     3061 aaaataatga atagattggt acaaggtgat gtagggagtg gtaaaacagt agtagctctc
     3121 ttagcacttg caaattgtgt tttaaatggt tatcagggcg ctttgatggc acctacagag
     3181 atattagcag ggcaacatta tatttcactt actgagtctc taaaagattt tggaataaat
     3241 gtaggtctat taataggtag tcttacaaaa aaacaaaagg atacagtatt ggaacaaata
     3301 aagaataatg agattgatat attaataggt acacatgcac taattgaaga taaggttgaa
     3361 tttaataata ttggtttagt tataacagat gaacaacata gatttggtgt tatgcaaagg
     3421 agcaaacttt cattgaaagg agcaaatcct gatatattag taatgacagc aacaccaata
     3481 ccaagaactt tagcacttat actctatgga gatttagata tttctattat agatgaactt
     3541 cctccaggaa gacagccaat agaaacaatt gctattgaaa aaagtaagcg agatagagca
     3601 tataataatc ttgtaagaag agaggttgaa tcaggaagac aagtgtacat tgtatgtccc
     3661 ttggtagaag aaagtgaagc catagaagcc aaatcagcag ttgaacttgt tgaggagtta
     3721 agagcggaat attttcacga cttaaggctt gggttgcttc atggtaaaat gaagtcaagt
     3781 gaaaaagatg aagtaatgag actttttaaa gataaagaaa tagatatttt agtttcaaca
     3841 acagtaatag aagttggtgt caatgtgcca aatgcaacat taatgataat agaaaatgca
     3901 gaaagatttg gacttgcaca attacatcag cttagagggc gagttggaag aggtagtcat
     3961 aaatcgtatt gtgtactaat ttatgattct aaaacagatg tatgtagaca aagaatggct
     4021 ataatggaag agaccaatga tggttttaag atttcagaga aggatttaga aataagagga
     4081 cctggggaat tttttgggac aaggcagcat ggtcttccag aacttaaggt agcaaactta
     4141 tttaagcata taaaaatact gaagttagca cagcaagaag ctcgatatat attgggtgaa
     4201 gataataatc tacaattaaa agaaaacatg gcactcaaaa aagaaattat agataaattt
     4261 aaagatacat tgaaagaaat ttcattaaat taattttaaa ttgtgttaaa atattactaa
     4321 tgtaataatg tgctgaaggg agattgtcat attgagagta atttcaggaa aagcgagagg
     4381 attaaaacta aatactccaa aaaatgaaga tgtaaggcca actacagata gagtaaaaga
     4441 gtctctattt aatatgataa attcatatat aatggaaagt gaagttctgg atttatttgc
     4501 tgggactggt tctttaggaa tagagtgctt atctaggggt gccaagtcat gtacttttgt
     4561 agatattagt aaggaaagca tagatattgt taagtccaat ataaaaaagg caagagttga
     4621 aagtgaaagt attattttaa accttgattt taagactgca atagataaac ttaaattaca
     4681 aaatagtaaa tttgacataa tttttatgga ccctccgtat tataaaaata tgtttataga
     4741 agctattgaa aaaattgata attctaatct attgaacgaa gatggaataa tagttgtaga
     4801 acatgatacg aatgatttat ttccagataa gatatgtaaa ttggagaaaa ctagagataa
     4861 gaaatatgga aatactacat taacttttta taagatggag gcataatatg gaaaataaac
     4921 ctagaaaagc tatatttgca ggaagttttg acccaattac taatggacat ttagatatta
     4981 tttgcagggc ttcgaagtta tttgacgaat tacaaatagg agtattaaat aatcctaata
     5041 aaaagggtct atttagcttt gatgaaagag taaaacttat agaaaaaagt acaagccatt
     5101 taaataatat aaaagttgtg acttttgatg gtttattaat aaattactgt caggaaaatg
     5161 ggattggagc attagttaga ggtgtgagaa gtggtgctga tgttgattat gaacttcaga
     5221 tggcacatat gaatagagag cttaatccag atatagagac tataatttta ccaagttgta
     5281 caaaatattc atttataagt tcatctttaa taaaagaggt tttgctcttt gatgctgata
     5341 taaaaaattt ggttccaaag attgttttgg aagaattaaa gaaaaaaact agtgggggta
     5401 attgatatat gaagatagat ttagaaatga ttgaactatt tgagcagtta gaggagatat
     5461 ttagaagtgc ttctacaatc cctttctcac ataaatgcac agtggataaa gatgaggttt
     5521 tagcaataat taatgatata agaactttaa taccagaaga agtaacacag gcagtatgga
     5581 ttaataaaga aagaaataaa ataataagtc aagctaagca agatgctaca aatatagttg
     5641 aacaagcgaa aaaagaagct gagagaatac aacaagaata tcaagataat atagaggaat
     5701 taaagaaaaa ttctgaagat gtagtaaagg catatgtaga atccagtgaa cctgttgttc
     5761 aagcagacca aagagcaaaa gatatagttg atagagctga gagaatagcc aatgagataa
     5821 gaattgggtc aattgaatat gcagaggatg tgctttctag tgttgagtat aatttaagag
     5881 aaatattaga agaagtaaaa agagataaag ctgaacttag acctaaaaaa taaattttaa
     5941 ataaataaaa atggagctat cacacaaaaa attccaattt atgtgtgata gctctctttt
     6001 aatatagcaa ttttttgtta tataattgtg ctttttagat tacttatatc ttattttgat
     6061 attataatgt agtttaatct tttatttgtt gaaatcaact tatttatttg tataaataat
     6121 ataggttatt ttcaaatttt tttaataatt ttaccactgt cttcttttag attagagtta
     6181 gactgtgcaa aattataaaa taatactaaa tcatgtgcaa ttatttggta tggcactaca
     6241 tgtagaaaat atagataata aaattaagga ggtagtatga aggaaagggc taaaaaaata
     6301 ttagatttta ttattaaaaa taacaatgta acatcacaag aactacaaga aaagtttaat
     6361 gtttcaaaga gaactattta ttacgatatt ctagctataa atgaacaact aggaaaaagt
     6421 ggtaatataa aaaatgtaaa acacaaattt atatttgaag gtaatttatg tgatgcaagg
     6481 aaaataattt caactgaaga agataaattt ttagatagtg attacagaaa aacttttata
     6541 ttaaacaaga tattacttgg cgaaaaaata tcaatagaaa aactaacaaa tgaaatgtta
     6601 ctttcaaaaa acacagtagt acaaacaata actgatgtaa aaaaatacct tcaaacaatg
     6661 gggttaagat tagagtataa gggaaagtac aaaattatag gtgatgaata cgttattaga
     6721 gagctgtttc tgataattgt tcaagaaaat atattggaaa ttaattctat aagtgaggaa
     6781 gtaagtagtt tcgatacaaa aggacatata aaacttactg attattcttt attaaatcta
     6841 actaagtttg tggaatttct aaataaaagg ataagagatg ggaaaacatt atactcatat
     6901 aaatatctaa atgaagctaa aaaaattagt tactttagta attgtaaaga acttttatgt
     6961 gaagaagcta atgaaaatga acaagcctat atatgcactt acataagttc acttcccagc
     7021 ttaaatagtg aagttaagga agatgttgtt gaagaatatg tagataaact tatagataag
     7081 tttgaagtaa atacagcaat aaaattagaa agtaagcatg agtttaagaa aaatatttta
     7141 aggcacctcc attcttctta caacagaata cgatttaagt ttcctattag aaatccaatg
     7201 ttagatgaga ctaagtataa acatgagtca ctttataaaa ttataaaatc aattatagaa
     7261 aatgaagaag agtttcctgt atttgagggg ataagagaag aagaaatagg atttatagca
     7321 gcgtattttg gaggatattt aagagggtct agggataatg gtctaagaag aaataaagtc
     7381 ttactagttt gtcctaatgg tcttatggta tcaaagagtt tggaaataca actatataaa
     7441 tacataccaa ccatagaaat agtaggtata gtatccatta agcaactaaa agaagtaaat
     7501 gtatactacg attacataat tacaactata gatatacaaa atgtaaacaa cgtaattgtt
     7561 gtaaatcctc ttttaacatc atcagatgtt caattactta tgaacaaact aataagtgta
     7621 aaagaaaacg agaagtattt caatcttgaa ttaataatac aagctataag aaaaaatgga
     7681 gttatcaata atgaagaagc actaaaagct gatttattaa acataataca caagatagat
     7741 gaaggagaga tgtatcaacc aatgttaaag gaattaatta atgcagaaag ggtcaatata
     7801 ataaaaagtg ttagagattg gaaagaagca ataaaaatag cctcaaagcc attgttagaa
     7861 gataactcta tagaagagtt atatatagaa aatatgataa agtcagttga gaaatatgga
     7921 ccatatattg ttttagctga tagatttgct ctaccacatg caagttctaa agaaggagta
     7981 aacaagcttg ctatgtcttt actaatagta gaagatgaag ttgatttatt aggaaaacca
     8041 gtaaatatat ttatggtatt agctgctgta gataacacta ctcacattag agcattagca
     8101 tcactatcag agatgatgta cgaggaagaa aatgtaaaac taataattaa tggtgataaa
     8161 agttcaatac tagaacttat aaataagcaa aattaggagg aatgaaaaat gaagatatta
     8221 gcagtatgtg gttttggtgt aggttcatca atggtattaa aaatgacaat acaaaaggct
     8281 ctaaagcagt taggtataga atgtgaagta gaaaatacag atataaattc agctagaggt
     8341 gctgattgtg actgtatatt cacaagtcaa gaattgttaa atgaattaaa aggaacttgt
     8401 aatgtagatg tatatccagt taaaaagtac atggatttag cagaagtcac agaagttgtt
     8461 caaacttttt taagtgataa aaatgataag aaatagtata agttttaaaa agtaaattta
     8521 agttaatcag aataaaataa aaacttacta aattcaaaaa tagtaagaaa gagaggaggg
     8581 atttagtggt agaaaactta aacagttgac ctatcactaa aaaatataat gcttgatttt
     8641 atttcaaata atattttacg taatccatct atgttaattg gattaatagc agcatttggt
     8701 cttgtactac agaagaagaa agcatctgat gttataaagg ggtcactact tgcagctttc
     8761 ggaattataa ttatggagac tggaacaggt atgttagtta gttcaatagc accaattaat
     8821 ggagctttcc aaagtataag tggtggtgca gtaacaaaag gtttaagtga tgttactttt
     8881 actgcaagtt atggtggaat tataggttta gcaatgtttg cgggtttagc tattcaccta
     8941 atgatagcaa gatttactcc tattaagaca atctttttaa caggtcacat gctttattgg
     9001 ttcccattca tatttgtagc ttcaggagtt gaagctggat taaatggtgg agtattaata
     9061 gcttttgcag cagtattatc agcactctat tggtcaataa tgccttggtt attaagaaaa
     9121 tatgtgtttg atgttatagg agatgattct tttacattag gtcatccatc tggttgttta
     9181 gcacttatat caggatttgt tgctaaatta gtaggaaata aagaaaaatc tacagaagat
     9241 ataaacatac ctcaaagttt atcatttttt agagaaatat ctataagtgg tggaatggca
     9301 gtgtttttaa tgtttatagt tgttgggata gtaataccag gtgcgtttaa agatagtaat
     9361 gagtttattg gaacgtatgc aataaagcaa ggtctttctt ttggagcagg tcttataata
     9421 atgcttcatg gggtaagaat gctaataaat caaataatgc cagcatttca aggtatatct
     9481 gaaaaagttg tgccaaatgc actacctgct tttgactgtc caatattatt taattataaa
     9541 ccaaatgctg tattaatggg attcttagtt gctatgctaa catctacagc tatgatttta
     9601 atatgtaact actttaatgt atttaatttg ctacttatac cacttgttat aacttcattc
     9661 tttgagtgtg gagcagcagc agtaatagga gaatcacaag gtggtatacg tggagctata
     9721 ataggtacag ctatagctgc aatatttatg gtagttttag taggaatatc tgctgtaatt
     9781 tactcaggaa ctattcaaaa ttggatacta atatttggag gtaatgacct ttcattattt
     9841 ggtataatca gtaggtttat aggttcacta tttggaggaa tattatagat atgaaattta
     9901 aatacataga tgagataaga agattaattg atataataga aaacgaagaa aaagtcaata
     9961 tggaaaaagc agtacaaatc tttgttgaag caatagaaag taaaaatgct atattttcat
    10021 ttggagcttc acatgcagga atattaacag aagaattatt ctatagagca ggtggctgtg
    10081 ttcttataaa tccagtattt gcaaaatctc taatgttaga tacagcgcct ataactcaca
    10141 caagtaagat ggaaagatta gaaggatatg gtacagctct agctgataaa actccaatta
    10201 aaaaaggaga tgtagttata gttcattcag tatctggtag aaatccagta agtatagagt
    10261 ttgctatgaa agctaaggaa aataaggcaa tagtaatatg tataactaat ttaagttact
    10321 caaaaagtgt atcttcaaga cattcttcag gaaaaaaact ttatgaagta tgcgatttag
    10381 tgatagataa tcatggtcaa aagggagatg cctgtgtaga gatagatgga ttaaaccaaa
    10441 aagttagtcc aacttctact gtaatagcaa ctactgtgat gaactctatt atagctcaag
    10501 ctactcagga attagttaat aaagggctaa agaatcctcc gattttctat agtgcaaata
    10561 ttgatggtgg agatgagtta aataagaaga tttttgatga atataaaagt gtaattcatt
    10621 atgaatatta aggttaaaaa aatatgttaa tgaactttga agtttatcaa caaagaatat
    10681 taataatata taataaatgc tatcatttaa aagctgtagg taaaaatcta cagcttttta
    10741 ttattgtaga ttaaaaagat gcgtaattaa attttaaatt ggagaaaata aattggaagg
    10801 tatcaattat aaatattaga aacgttgtca tagaaaaaaa ctatattaaa aaaaagtaca
    10861 tagaaaaaac tgattagaca ataaatacat gctttgataa tattttaaat ggagtgaaaa
    10921 tgtgtagaat tataatgaaa aaagaaaata aaaatgaaaa aagaaaataa aatggaggga
    10981 ataaaagtgg gtatgttaaa aaaattatgt tcactagcta tggtagcaat tatgactaca
    11041 acacttataa cagggtgttc aagtggaaat ggaaaagatt caaaaaagga tggcgaaaaa
    11101 gaaaaactag ttctttggat gccaccagta gaaaataata tgaaggaagt ttgggaccct
    11161 attttagata aatttgagga aaaaaataac tgtgaagtag atttacagat aattccttgg
    11221 gataattatt ctgaaaaatt tgcaacagct ataagtgctg gcgaaggtcc agatgtagga
    11281 tatatgtatg cagaaatgta tccacagttt atagaaagtg gagcagtaga agatttgact
    11341 aactatgcaa ctaaagagga taaagagcaa tctatttata taaaaacatc tgaaatgatg
    11401 ggtggaatgt atggtatgcc attccaagca gcaaatccag gggtattata ctataacaag
    11461 gatattttag agaaactagg agaaaaacct cctaaaacat gggaagattt taaaagaatt
    11521 tgtaaaaaag caacgcaaga tactgatggt gatggaaaga tagaccaatg gggattagct
    11581 cagggatggg gagctaaaac ttttggtaac ttaaactgga actggtatcc ttatttatgg
    11641 caagctggtg gagatatatt taatgatgat ttaaaatcgg ttaaatttaa tgataagagt
    11701 ggtttagaag cagctaagtt cttaaaagaa ttacaagcat atgtgcctga agattcttta
    11761 tctaaagata gtaatgaaat gatagaaagt gtatttggac cagggaaagc agcatttaca
    11821 ataatgttat cttctgctgc aacaagtgta tttgatgaat cattcccgga tttaaattgg
    11881 ggatttgtaa ctggactaga agataagaaa gcagcaactt ttggagcagt agaccattta
    11941 tctttaatct catctgcgaa aaataaagaa cttgcataca agttaataca acatatgtta
    12001 agtgttgaat caatgacaga atttcataaa gcaataccaa gagcaccaat aacaaaaggt
    12061 gaaccatatc aaggtgatga aagattcaaa gaaatggtgg aaaatgacaa aaatgtatat
    12121 agaccattag tagtaggacc tcatggagtt gaaatatatg aatatttatg gaaagaactt
    12181 caaactatga tttcaggaga taaaactcca aaacaagcgt tagatgatgc tgcaaaatat
    12241 tcaaatgatt tattagcaca ataaaagttt aaagctgtaa tatgttgtaa agtactttac
    12301 agattaaaat tagcattgta tttataaata caatgctaat atttatattg gagaggataa
    12361 aatggaaaac ttagttagag agtttgaaga aaattcaaaa agaaaaaaaa caaaattaaa
    12421 tattaaacca tatatgtata tattgccatt gggaataatt ttagtggcgt tttatgtgat
    12481 accaataata atgtcaatat actttagttt tacaaagtat aatattatta gtccagctac
    12541 ttttataggt ttagaaaatt ataaaaaatt atttacagat gaaacattaa aagtatctat
    12601 tataaatact attaaattta ctgttgttgt agtaccttgc caaactatat tatcattgat
    12661 tttggctgtt tggattacag gaaaaggaaa tagtaaaata gcttcatttg ctaaaggtgc
    12721 tatatttata ccagtattat catctatggt tttgataggt atggtatgga gggctttatt
    12781 gaatggagaa ggttctatta tatatcaggt attaggtgcc tttggaatag aatcatctaa
    12841 acttttagga gatagtaaga cagcattacc tacacttatg tttatatcta tgtggaaaaa
    12901 tatagggtat ttcatggtaa tttatatttc agctataatg aatttaccaa aacactgcta
    12961 tgaagtagcg aaggttgatg gggctacaaa acttcaagaa tttataaaaa taactgtacc
    13021 tttattgaaa ccaactacaa taatggttgt atttttaggg tcgatatggt cattacaagt
    13081 atttgactta atttatactg tgacaggtgg aggtccaggt atatctacta tgagtatagt
    13141 tatgcatgca tttaacttaa actttaaaaa ctttaattct ggatatgcaa tgactgtagc
    13201 aaacgtatta tttttgttaa tagcagtagt ttcgatttta caaaataagc ttgtaaaacg
    13261 agacaattca gatttttaga aaggtaggta taatatgaaa acagcaagaa gattattaag
    13321 tgatggttta cttttattta tagcgtgtgc aagtttggta ccttttatat acatgttgat
    13381 aatatcattg aagataacat ataactcata tagtttggat atatcttttt caacagtaac
    13441 ccttcaaaac tatatagata tttttactaa aaaaggattt gcacaatatt tttttaatac
    13501 agcaatagta tctttttcag gtgtattatt aaatttagta tttagtacat tagcaggata
    13561 ttcttttgcg aagatggatt ttaaaggtag tgataagtta tttttattta tgataatgac
    13621 tttaattata ccttcccaag ttacaatgat accactatat ataataatga aacacttagg
    13681 atggataaat agttatcttg cacttattat gccaatacca actgcatttg gcgtatttat
    13741 aatgagacaa gctattttag gagtaccaaa agagttatta gaatcagcaa agatagatgg
    13801 ttgttcggat tttagaattt taatacaaat agtgttgcct ttaattaaac cagcattaat
    13861 tacactagca atatttactt ttatgggagc atggaacgag tttatgtggc ctcttatagc
    13921 tacaactaaa gatgcaatga gaacattaac agttggatta tctactctta agacattcca
    13981 gataactaat tatggacaga tgatggcagg ggctacaata acatttttac ctccatttat
    14041 tttctatttg attttacaga gtaaatttgt tgaaggtgtg tctttgtctg gtataaaagg
    14101 ttaaatagtc taatttaata atatataaag aaacttatat aaatatggag gaatttaaac
    14161 tatggaaatt tcatatgtac tgaaagattg cacattcaaa cacaataaat actcagttgt
    14221 agaagctgaa acttggaaag ataaggattt ggatattgta gtagctaaag gtgaaaaatt
    14281 tgcatttcaa attatgttaa aagcaacaga agaatttaat tgtacaattg ataaaagtag
    14341 tgctatatca tggaaaggcc tgggaaatag agtaaggtta gcattaaata tgcctaatag
    14401 tttagaaaat aattttagta taaatatatt aggatatgtt caagatgata caaaggcatt
    14461 tgtgaatgat gctattttaa gggataaaga tgtgttagta gagcaatatt tgcctcaaac
    14521 attttggatt gaaggtcaag taccagaaga ttttgaagaa aataaccttg atataggtat
    14581 tgatatattt aaaagctttg gatatgaaga tgaagaaaaa gtttgtacaa ttgatgttcc
    14641 tgtaaaagta agaaatgttg tgctaaagcc tttagatgaa agcaagttct ttttagattt
    14701 atggcaacat ccaagctgtt tagctagaat gtataaagtt gagttatggt ctgatataca
    14761 ttttgaaata gtcgataact atttaaaaga attagcatct ttaggagaaa aagttgcaac
    14821 agttatagtt tcagattatc catgggcagg gcaaagttgt tataaagttt ataaaaatcc
    14881 atcaaactta tatgaatata acatggtaag tgtatcaaaa ggattggatg gaaagattaa
    14941 atgtaacttt gagagtatgg ataggtatat aggcatagct gataagtata aaatggctaa
    15001 ggagatagat ttatttggtc tattaggtaa ttggtgtgct ggtgagtttg gaaatcctgt
    15061 agaaggttat aaggacccta taagagtaag atattttgat gaaaaggata aagtatttaa
    15121 atttataaac aatacaaatg atttaaaaga atatatagga ttagtattaa atcatttaat
    15181 agagtgtgga ttatgggaca gagtaaggat tatagctgat gaaccaaata atccagaagt
    15241 agtcaaagag tgtatagaat ttataaattc tactgtaggt actcatcaag ttaaatataa
    15301 atcagcaact cacgaccaaa actttttaga cagagctaaa gatgaaattg atgatatgtc
    15361 aataaattta aaacttacta tacaaaatta taaagatata gaaagtctta agaaaaaaat
    15421 caatgacaag ggtgggatat tgacttggtt tgtatgctgt tttccagaaa aaccaaatag
    15481 ttttttaagt tctccttttg tagaaaatag aataatagga tggtatactt attactttgg
    15541 tcttgatgga tttttaagat gggactataa tttatggaca gaagaccctt ggaaagattc
    15601 aagttacaaa ttccctattt ggaaagctgg agatatgttc tttgtttatc ctggaaaaga
    15661 tttaaagcca gttagaagtg tcagaatgga gaatttaaga tttggaatac aggattttga
    15721 gttatttaca atgttagaaa aagaaaaggt aagagagtat atagaagttg agcttatgca
    15781 agaattatta aataaaaaag aaaatgctga aattaaaggt tttggtgaca tagaactagg
    15841 atattcactt gataattgga gatatgataa agttaaaaaa tatgtattag agttgttaga
    15901 cagagtataa aaaatataag agtgtctcaa aatgaacttt ttagttcatg aggcactctt
    15961 tttgtatgaa atcaatatag tttcattgtt tcaacaatga aaaataggat tatttctatt
    16021 ctaagacagc cctttagttt tttagttgta ttcaatgttt taaaactaac tataaacgct
    16081 ataaagttta taaatgacaa taattatata taacatgatg tactttaatt tgctataata
    16141 gggattgaat aatattatat ggaagataac attgtttggg gggattaata taaaaagaat
    16201 aggtattatt ataatgtttt ttttaagtat atcttttatt ggattttatc ttgtaataag
    16261 agataacagt gatacagtta agatgcaacg agggataaat ataggtaatg cactagaatc
    16321 acctaaggat tttccgtggg atgtaaaaat gtcaaataag ttttttgatg atataaaaga
    16381 tgcaggtttt gacacagtac gtataccagt aaggttttct gattatacgt cagattctga
    16441 taattttaaa attgatgaag aattttttaa aaagattgat aaatatgtag attatgcatt
    16501 ggataaagac ttaatagttg tgttggattt acaccatttt gaagaaatta tgaaagaacc
    16561 aagagttcat aaagaaaagt ttttaaaaat atggcaacag atagctaata ggtatcaaaa
    16621 atatgataaa aaattggtat ttgaattatt aaatgaacct aaagaaaatt tatcttcaca
    16681 attactaaac gaatacattg aagaagcaat aaagataatt agaaaaacta accccaaaag
    16741 aactattata gtaggaccat ataatttcta tcaaattgat tatttgaatg aattaaatat
    16801 tcctaaagat tctaatatag tagtttcttt tcactattac gagccaaatg attttgcatt
    16861 tcaaggaaat atctatcata agggatttga gcacttaagc aatattactt gggaaggaac
    16921 aaatgaacaa atggattatt taaaaaaaag gtttgatact gtggaaaatt gggcaaataa
    16981 aaataaggta aaaatcttct tgggtgaatt tggaataaca aaagaggcac cagaagtttc
    17041 aagaagagca tggataaaag ctgtaagaga agaagctgaa aaacgaaact tttcatgggc
    17101 ttattgggag cttgcatctg gatttggtat atacaatcaa attgaaggga cttgggatag
    17161 agatatatta agtgcattaa tagaaaagag gtgattttat gaagaaaaat tcaaaaaaga
    17221 tacttataac tttaactatt attactaatg tcatatatat attgtggaga attttttaca
    17281 cagtacctaa agaagaaggg aagtttgcct taatatgtgc cataatactt ctttttgtag
    17341 agataatggg tatgatggaa atgtttgtgc attattatgg tatgtcaaat attgagtatc
    17401 ctgaaaaacc aatcataagt gaagaacttt atccacatgt agatgtattt attgcaacat
    17461 ataatgaatc tgttgatttg gttagaaaaa ctgtaaatgg atgtattcat atgcagtatc
    17521 cagataagaa aaaggtacat atatacatat gtgatgatgg aaatcgtgaa gaaatgcgta
    17581 ttctagcaga aaaaatgggt gtaaattaca taactagaac tgaaagagaa ggagcaaaag
    17641 ctggaaattt aaataatgca atgcaacata caacttctcc tttgattgct acatttgatg
    17701 cagatatgat accaatgcat gactttttaa ttgctacagt accatacttt ttaaaaaatg
    17761 agcaagctaa aaaagatgga gaaaaagagg agtatgaaaa ggttggtttt gtgcaaactc
    17821 cacagagttt ttataatcca gatttgttcc aatttaatct gcactctgaa ggaaggattc
    17881 caaatgagca agattatttt tacagagata ttcaattggc tagaaatagg acaaattctg
    17941 taatttatgg tggtagtaat acagttattt caagggaagc acttgaagaa gttgatggat
    18001 tttatactta ttctattaca gaagattttg caacaggtat cttaattcaa agtaaggggt
    18061 atagatgtta tgctattcca gaagtacatg catcaggttt atctcctaca gatttaaaaa
    18121 gtcttattaa gcaacgtgaa agatgggcac gtggatgtat acaaactggt agacgtctaa
    18181 atattttgtt taggagaggt ttaggttttt ggcagaaaat aagttatatt tcttcaatta
    18241 cttattggta tgcaagtata aaacgctttg tatatataat ggcacctatt ttattttctg
    18301 tgtttaatgt gattgtagta aaatgtacgt tacttcaagt attggtattt tggttgccaa
    18361 tgtatatttt aagtagttta tctcttaaaa tattttctca aaatataaga aatactagat
    18421 ggaccaatat atatgaaact ataatgtttc agtctttaat gccagcagta atacttgaaa
    18481 cgtttgcaat atctaaaaat aaattttcag taacaaataa gagtaaatta gaagaaaata
    18541 gaatgtataa gtttttacag ggaatacctt attttattta catggtctta tctatcatag
    18601 gaattttaaa aatgtttgta gcaatattta aaatgagttc aatgacatat tcagttgtat
    18661 tattttggtt gattggaaac ctttttaacc ttgttatggc tacattattt atatcaggga
    18721 gacagcagtt aagaaagtct gagcgttata ttgctgaaat tgattttaag ttaaagcaaa
    18781 attcttatgt tctttcttca aaaacaattg atatatcaga aaatgggttt gcatttttgc
    18841 tagaaaatcc agaatatatt tcaccagaag aagagtttga ggtagagttt agagaaaaat
    18901 ctggaaatga aatgtatata gccaatatga aagcaaagat tgttaatgtt gtggaagtaa
    18961 attcaaagtg gaaatatgca gcttatatta cacatattga agatagtgaa atagataatt
    19021 ggatgtgtat tgttcacgat agaataccta ctttaccaat gacaatatct aatcaactag
    19081 gtttctttga tgatttgcaa attaatgtga aaaaacgtat tgaaaaaact agaacattat
    19141 cacgaagaag tccaagaatc aatatgaatt ttcagatgga tataaaaaat acaggtaaat
    19201 taagaatagt gaattttaat tatcaatatg tattattaaa ttttgagaat aaaagtattt
    19261 atcctaaaga aatagcattg gaaattaatg aaaatattgt gttagaatgt gatttgtgtg
    19321 agggtaaaat tgatgaaaga ggtatcttat ataaagtaaa taacattgac agcataatgc
    19381 aaaatttctt tttaagagat gaaatgatgg attggatatt acaaaataag acaattcttg
    19441 tttcaaagcc tagtgaaaag aaggaaaaaa gtatagatga gtttgaaccc atggaatata
    19501 tttaggagag atgaaatgaa gactttaaaa gtgttttttt gttctacaaa atatcttatt
    19561 ttggtgttta ttttttttat aaatactact tttataagtt cagcaacaag tgataaaatt
    19621 ttagatttgt cctttaaaaa gatagagact gacttaaatt ctaagattac atatgaagat
    19681 acaggtgtaa aaatagaaac ggatagttct aaaagcgata aggaacgtta tttatatatt
    19741 taccaaaata taaaagaaaa ttggagcatg tataataatt tttatattga aattcaaaat
    19801 aaaaataaaa gttctcagag aataaattta agcatacaaa gtaaaaatat gcttgaattt
    19861 agattaaaag aaggtagtga agtattttta gaaggtaaaa atataatata ttcagataaa
    19921 ataaaagaag gatgcataga agtaccaggt gaatttgaag gcaaaattta tgtgaacttt
    19981 aatagcctta taaatgagga aagtaatgtt gtgctggact caaatatgct ttcaaacatt
    20041 gttagttggg gaattacgtt tattccatct gatgaagaac ataatattgt tattataaaa
    20101 aagatatctt tattgtctga agagaaatta agatttttaa ataatattaa aataattggc
    20161 gatgaagaag tacaaatacc agtattgggt caaagtataa gtcagtatga agtattaggt
    20221 cttaaaagtg attctaaaat taagtactct cttatgggaa aacaagataa tgtaagcata
    20281 tctcaaaaag gtaaattaac attaagtaat aaagccaaac ctggacaaat tattttacaa
    20341 gttaatgttg atgataagtt taaaattgga aagaaaataa cattaactga gtcttggagt
    20401 gttaataaaa aagataagga tggtgttcca tatacattag tatatcctga gcaatcacca
    20461 acagtacaag atatgaaaaa aataaacttt atgaataaca tcataacgtt tgttcgaatt
    20521 ttatttgtat ctctagtaat aatatgcttt ggaatatatt tatattggaa aaaatgttca
    20581 aaaactaagt aaagagagga ggttagattg tgttattttc aagtttaatt tttttatttt
    20641 attttttacc tataactttg gttttatatt atgtatttag gtttaaccga acgatacaga
    20701 acatgatatt gcttgctgtt agtttatttt tttatgcttg gggagagcca aaatttgttg
    20761 ttatcatgat agtttccata ataatgaact atatatttgg tttactggta gatagatata
    20821 gggaaagtaa aataaaagta aaagtttttt tagttttgat gtgtgcatac aatataggtg
    20881 ttttatttat atttaaatat ctagcttttg cgctaagaaa tattagtgaa cttattagta
    20941 ctgaattgac tatacctagt attgttttac ccataggaat ttcatttttt acatttcagg
    21001 gaatgtctta tgttatagat gtatatcgta gacatggaga agtccaaaag aacccttttt
    21061 atgtgggatt gtatatagca tttttcccac aattaatagc aggtcctata gttagatatg
    21121 agagtgtggc agaacaaatt ctcaatagaa aagaaacttg gaataaattt tctattggga
    21181 cttgtaggtt tataacaggt ctaggtaaaa aagtattaat ttcaaataac atggctatag
    21241 tagcagacta tatttacact atgaatagtc aaggagaaat agctgcatca cttgcatggc
    21301 ttggttcaat agcctatact ctgcaaatat tctttgattt ttctgcttat tcagatatgg
    21361 ctataggctt aggtcttatg tttggtttta aatttgaaga gaactttaat tatccatata
    21421 tatcaaaatc aattagtgag ttttggagaa gatggcacat ttctttggga atgtggttta
    21481 agagctatat atactttccg cttggaggtt ctagagtagc aaataaagat attatgatta
    21541 gaaatatgtt tatagtgtgg ttatttactg gaatatggca tggagcagaa tggacatttg
    21601 taatttgggg gattttaaac tttgttttcc taattataga gcgatttata atgtttgaaa
    21661 agattgaaaa tcacaatttt ataaaacata tttatgcttt attagtagtt aattttgggt
    21721 gggtattatt taggtcacct aatttaaaag aagcatataa ttattttaaa gctatgtttg
    21781 gaggcaatgg agctatatgg agtgattata catatatgtt tttaaaagaa tactgggttt
    21841 tctttatatt tgcatttatt tttagtgtac ctattgcaaa gaaaatcaat aagtttgttg
    21901 ttgaacaagc taaatatagt atgatattta acgtatttta tccaatctca gctattttat
    21961 tgttttttat ttctgtgaca tatttagtaa ctggaagcta taatccattt atttatttta
    22021 atttttagtt gggaggaaaa gtatgaatag attttttaaa gtaagattaa taactgcaat
    22081 tatgttctta attattttat tcgctttctc tatctttaat gtttgttatt ctggaaaaga
    22141 tacgatacag gaaataaaaa attcaatagc tgaaaaaagt agtttaaaag atacaatatc
    22201 atctattgat aatacagtga atgacaaagt aatatacaaa aatacgttca ttgaaactta
    22261 tggctattta caaaaattaa tgggtaaaaa tgagttttca aaatttagtg ttgtaaaaga
    22321 tacttctgga aagatgcatt atacatattt tgctaaagct ccaaatccag ttggtgtgat
    22381 agcagatagg gtaagaaaat tttctgatga aatggaaaag caaaacacta aattggttta
    22441 cctaatgact ccagataagt atataaaagg agtgacaaaa tttccaaaag gaatacctta
    22501 ttcttatagt aatgagacag cagataattt tttaaatcaa ctaaaaaagt ataatgtgga
    22561 ctatattgat tttagagaaa atattttaaa gagtggtatt ccaaaagatg atttattttt
    22621 taaaacagac catcactgga aagtagaaac ttctttttgg gcatttggag aattagttga
    22681 gcaacttaat aaaaaataca atatgaattt agatgaaaat cattattata gagataagga
    22741 caattataat tcaattgtat atcctaagtc atttcttggt tctatgggaa gaaagactgg
    22801 tatcttatat ggtggtatag atgattttac attaatttat cctaaattta agactaatta
    22861 tacttattat acagattcta aatctcaaaa atttgaatta aaaggaaggt ttgaagagag
    22921 tttaatatta tcatatcctt ttaatgcaga ccttgattta atggatggtc aatcagataa
    22981 gtattttaca tatttattgg ggaacagacc attagttaaa ataaaaaata tagaaaatcc
    23041 agatggatta aaagtgctgt ttgttaaaga ttctctaatt gtaccaacag cagcattttt
    23101 ttcctctgtt tgttcatcta tagatatgat agaccctaga tattatgatg gagatatact
    23161 agaatatgca aaatcccatg actatgactt tgtttttata tctgtatatc cccaaaatct
    23221 tacaaaagaa ttttttccgt tttgtgagta aatttctaaa aaagttttct gataaaataa
    23281 atggctaact ggtaaaaagt tagccattta ttttgagtgt tattctaaga aatatttttt
    23341 ttactaaata cttctttatc tatttttcca agtgattttc ctacagtaat tgatgatacc
    23401 atagcaccat taacatttat catagtacgt cccatatcta gtatagggtc tattgcaagt
    23461 ataccaccaa ctaatggaaa ataagaacca agaccaactc cagagataac aactgaaacg
    23521 gccattgtag cagttcctgg aagaccagct attccaaacg aactaattgt tataacaatt
    23581 aacaacataa cataaaagct catatccatg tttactccag ccatattggc aagtgttaca
    23641 gccattaaag caggatatat acctgcacaa ccattcatac ccatatttgc acctaaactt
    23701 gctgtaaagc ttgatacacc ctcatcaact ccgtgatttt gtactagact ttctatcgtt
    23761 acaggcaatg ttcctaaact agaacgtgaa gtaaatgcca gaagtaaagc ttcagttgaa
    23821 ttttttatat aagtaatggg atttagacca tttaaagtta taaaaattaa atgtataata
    23881 aataatattg caactgatat ataaagagct aatataaact gtaaaacaga tactattgaa
    23941 ctaatacctt gtgaagttat tgtatttgca agcagagcaa ccacagcata aggcataaat
    24001 ttaataacag tcatagacac acttacaatt attttgtaag atgcttctat aaaacctata
    24061 aatggtttta ttgtttcaaa atgtttttta cttaatctct ttattgaggt agctataaat
    24121 gtagcaaata ttattgtagc tacaatattt ccatttgcca tagccattac aggattagat
    24181 ggtagtaatc ctcttaaagt atccacaaga gagtttattt ctttaagttc ttgattttca
    24241 actaaagtag gcatattagc tcctacacct aacttcatta tatttgcaac gataattcca
    24301 acaattgcag ctatagcagt agtagcaaga agcataccta aagatttaaa tgtcagagtt
    24361 ccaagattgt tatctttcat atttataatt acttttataa tggataagaa aactaaaggt
    24421 actactaaca tttttaataa gtccataaat ccagaaccaa ataggctata ccacttactt
    24481 acttcttgta gccaaatgac ttgagttgta tcattaggaa aaccagcaaa agcttgtatt
    24541 atgattccca atatgagtcc tagtactgta gcatatattg ttctcaatga aaattttatt
    24601 tttttctttt caaattgtct aacaataaag aacgtaccta gaagtaatat tatgaaaaaa
    24661 attgttttaa aatctgtaat cataagaaat tgtgataaaa atgaactttc caatattttt
    24721 ctccttttat ttaaaaataa atttttctaa gtttaaatga tatcaatata ttaaaaaaat
    24781 gtcaatatta tatcacaatt tacttaaata gagtaaaaca agttgatttt gaaaaatgtg
    24841 attaagtaca gaaaaatagg gtataataaa tgtttaaaga atttgaattg tggtaagaaa
    24901 ataaaaaata gcttttataa gatttgattt caaattaaaa tgtgttaaat aaataactaa
    24961 aaattattat aaaaaatgtt ttcatagaat atatatcaaa tgaaaagctt aaaataagtg
    25021 aagaaattaa ctagacatca aaggagagat aaaatgagag taataataat tggtggtgtt
    25081 gcagctggta tgagtgctgc agcaaaatta aaaagaataa aacctgagta tgaagttgtt
    25141 gtttatgaaa aaactgaaat agtatctttt ggtgcttgtg gattaccata ctttgttggt
    25201 ggatttttta atgatgctga tgaattatta gcaagaacac cagagaaact tagagaagct
    25261 ggaattgatt taaatatatt tagagaagtc gttgaggttg atagtgaatc taagaaaata
    25321 aaagtaaaaa acataaaaac agatgaaata tatgaagatt actatgataa attgatgata
    25381 gcaactggag caagaagtat tatgcctcct ataaaaaaca taaaattaaa gaatgtttct
    25441 acattaaaaa gtttatatga tggagaatat ttaaaaaaac ttttaagtaa tgaagataat
    25501 aaaagagtga caataatagg agctggtttt atagggttag aagcagttga ggcatgtaag
    25561 aaattaggta aagatgtaga tgtaattcag ttagaagata gaatattgcc acaagtattt
    25621 gataaggaaa tgacggatgt attagaagaa gaaataaata gacataatgt taatttgcac
    25681 ttagatgaaa tggttgtaga actttgtgga gaagataaag tagaaaaagt tataacaaac
    25741 aaaggggaaa tagatacaga tgtagtaata attgctactg gcgtgagacc taataccgag
    25801 tttttgtcaa atactaatat aaaaatgcta aaaaatggag ctatagtagt tgatgagtat
    25861 ggaagaactt ctgtagaaga tatatactct gctggagatt gtgcaactat aaagaatata
    25921 gtaagtaatg aaaatgtata tgttccttta gctactggag ctaataaatt gggtaggata
    25981 gtaggagaaa acctagcagg aagagaaata tcttatcaag gttcactatc ttcaagctgt
    26041 ataaaaatta tggatatgga ggcagcatca actggaatta cagaaaggca agctaaagat
    26101 ttaggcataa atgtcaaatc taaatttatt tctgattata atcaaactca ttattatcca
    26161 ggaagaaata aaatatatgt aaagcttatt tatgatgcag atacaaaagt tattttaggc
    26221 ggacaagtgg ctgggtttaa ggatgctgtc caaagagcaa atgtattggc agctgctatc
    26281 tttggaaaaa tgaccacaaa tcaacttgga atgttagatt tatgttatgc tccaccattt
    26341 gcaagaacat gggatgtttt aaatgtagct ggaaatgtat ctaaataata tttagattat
    26401 agctaatata gttgacaagc aattaaggga atgaatttac ctaaactatt tttatcatgg
    26461 taaaattcat tcctaaattt ttattccaaa tttttattcc aaatttttag aaataaggat
    26521 tccattaaat tattcatcaa aatttaaacg caagcttaat ctatcttctc caagaatagt
    26581 attttcaaaa acattagtag cattttctaa atacatacta ggtatgtcta aagaaggact
    26641 aaaatgagtc agtaaaagtt gtttgacatt tccaagtttt gcaagattag cagcttctct
    26701 aaaagtcata tgtttgtttc taacagcttt tgatatatct aaatcatcac catacatggc
    26761 ttcacatata aataaatcac tatcttttat gaaatctggt attgattcta taggtcttgt
    26821 atcggttgta agacttatct taactccctt tctcaattct cctaagacca tatctggagt
    26881 gtattgtttt gaatctaaaa ctatattttg accttcttgg agtttgttcc ataatatttt
    26941 aggaacttcg tttttagttg ccttatctat atcaaattta gggtttcttc taaaattaaa
    27001 actgtatcct aaacactcta tagagtgttc taaactgatt gttgaaattt ctaaatttct
    27061 taaaacttta ttatctaaag aaaaattgcc ttgagggttt tctataattt ttaaagtgta
    27121 tggtacatat tctactaaat ttctcataga acgtatacaa tcgactattc caactggacc
    27181 aactatagtt aaatcacttg tacgaccact attcccaatt gttgataaaa gtcctaaaag
    27241 tccaaatata tggtcaccat gtaaatgggt tatacaaatc aagtctatat ctttaaagcc
    27301 acattttttt agcttcatag atacctgagt accttcacca cagtcaatta gtatttttct
    27361 acctttatag tttataaata cagaggatag aaatctgttt ggcataggta catttccacc
    27421 acatccaagt aaagttaaat ctatcatttt ttcccctttc taaaattaac acaaatctgt
    27481 tgtttgtttt tattatgata tcctattata attattttaa cattaaatat aacaataaat
    27541 gtgtatttga gtcaagaaat attgtcaaat aaagttttta tatagcaatg tataaaaatt
    27601 tggaaaacga aaagtgaaaa ttatatttag gagtatttaa aggaggaaaa tttaatgaaa
    27661 gtattaaaga aaattactat tttgtcatta attacattaa ccttagttgg ttgtagtaac
    27721 ggagaagaga aaaaagtggt tgaaggttct aagaaaactc aaaattctaa aaaagatgaa
    27781 cagcctataa tagtacataa agatgcaaat atattgaaag ataggtatga gccaccaaaa
    27841 ggatttaaaa gagttgatgt agaaaaaggt agttttgaat catttcttag gaatactaag
    27901 ctaaaaaatt atggagagaa agtaaagtat tatgatggaa gaactaagga atcaaaaggt
    27961 atttatgata gtgtatttga tgttgatatt ggaaatagag atttacatca atgtgcagat
    28021 gcaattatgc ttatgagagc agaatacttg tatcagaata aactatatga tgaaataagt
    28081 tttgattttg ttgatggttt taaggcgaag tattcaaaat gggcacaagg atatagaatt
    28141 tcagtaaaag atagtggttc aagctggtat aaagcaactg aatattcaac atcttatgag
    28201 agtttcagaa agtttatgga tatagtgttt gcatattcag gaacgttatc tcttgaaaaa
    28261 gaattggagc ctgtaaaagt tgaggatatg aaaataggag atgtgtttat agttggtgga
    28321 agtccaggac atgcaagtat aattatggat atggcagaaa atgaaaagac tggtgaaaag
    28381 gtatttatga tagcccaaag ctatatgcct gcacaacaaa cgcaacttct tattaatcgt
    28441 aaaaatccaa atttgagtcc ttggtatagt ttagattttg aaggtgattt gacaatacct
    28501 caatggactt ttaaaagaga ccagttgaaa agattttaaa ataataattt tatataataa
    28561 aagaaaaata tagatgtgaa aaaatgtaat tagattaaaa ctaattggat atggacaaaa
    28621 ttaaattgat agataaatat taaaaattac actaattaat cttactatag tttaatattt
    28681 atctaaaaag ttttatgtaa aatataaatt ttatattaaa gctaaaacta atttgaggag
    28741 gctaatttat attgaagatt agaaggtatt taattttggt aattataatt ctaattacaa
    28801 caagtcagag ttttatttct tatgggctag agcgatttga agaaataacc atttttcaca
    28861 ctaatgatat acatggaaga tatgctgaag gtgatgacca tatacaaata ggaaatctag
    28921 ccacattgaa aaaagaaact ccaaactcaa ttttagtaga tgcaggagat tgtttgcatg
    28981 gacttccaat agtaaatatg gatagaggga aaagtgcaat tgaattaatt aaatgtgctg
    29041 gatatgacta tattactcct ggaaatcatg actttaatta tggaaaagat agactgtttg
    29101 agttatctaa aagtgctaat ttaggagaga atactttaaa atttttatca tcaaatattt
    29161 ttgaaaatgg gaaaaaagtt tttgatagta atgatataaa agaaatagat ggaataaaaa
    29221 taggattttt tggactctca acccaggaaa caaagagcaa aactggattt aaaaatgtgg
    29281 aaggattaga gtttaaaaat cctatagaaa gtgcaacaga acaagtaaat gaattaaaag
    29341 gaaagggtgc caatgtaata gttgcaatat gtcatattgg tacaaatagt ttaagtaagc
    29401 caaatagtat agatatagca aatgaggttc aaggaattga tttgattatt gatggacaca
    29461 gccacactaa gtttgaaaat ggtaaacaag tgggtgatac attgattgta agtgcaggac
    29521 aatatttaga aaatattggc gagataaaac taactttgga tactaatgat gtagaagata
    29581 taaaaataga aaataaatca gctagattaa ttggaaaaga agaagcatta aagtataagc
    29641 ttgactccgt tgtaagtaaa aaggttgata aaataaaaga acaacaagaa aaaatattag
    29701 ataaagttat aggggtaact acaaatactc tagatggttc atatgaaaat gtaaggacaa
    29761 aagaaactaa tttgggtaat ttaataagtg acattttact agataaaact aaggctgata
    29821 tttctttatt taatggaggg aatattcgag atacgataga aaaaggcaat ataacaagaa
    29881 gagatattgt agatgtattt ccttttagca atactatagt taccaaagaa cttacaggag
    29941 cacagattaa agatgtattg gagcatggag taaaacttta tccagaaaaa agttcagcct
    30001 ttttgcaagt gggaggaatt tcatattact ttaatcctaa acaaaaagaa ggcgaaaaaa
    30061 taacaaatat tcaaaaagaa ggaaagccat tagatttaaa taaaaagtat gtagttgcaa
    30121 cgaatgatta tatagcatca ggtggagatg aatttccttg tttttcaaaa gagccagtac
    30181 taaaggaatt tggaaattta gagagtgcag tcattgaata tatagagtac aaaaaagaaa
    30241 tatcaaaaaa tgtagatgga agaattggta ttaaatctaa agatgagtta ataagcaaga
    30301 atcatgagag tgatttgata ttaagtgaaa atgaaaaaaa tatcaatgat aatgaagaga
    30361 atctatctaa taatataaaa agctctgata gtgataaaaa taaaagctta gataaaacaa
    30421 gttctagaga aagttctaat ctaggaatat ctaaaagaga agctaaagaa aaatctccta
    30481 aaacaggaga tttaggtttt tcaaatagta taattatatt tatagtttca agtacattga
    30541 tatgtttatt gaattttaat caaaaagagt taaaggataa aaagtcaaaa taaaaattaa
    30601 tgagcaaaaa ttagtagata gtaatttaat tttagataat agtaaacagc aaaggaaatt
    30661 agttatataa atactaattt cctttgtttt atgttttaac atgattactt ttaaataaaa
    30721 ttgtatttta tgaaaaataa ttatactaaa tggcatagta ttatggggat ttttagtttt
    30781 tattcaagta aatgaaatca tattaacaac ttctgatata tttacaaatt gataaaaaaa
    30841 tgattttatt agtatgatat aatttagcta gtatgtatga taatggagga tatagggatg
    30901 aataatatat tacttttgga agatgacaaa tctttaaata gaggaattag ttttaaatta
    30961 aaaaaagaag gatacaatgt ttttagtgca tttggtatag aagaagcaaa aaatattttc
    31021 gcaaaagaag agatatgctt aattatttcc gatataggtc tacctgatgg tagtggtttt
    31081 gacttttgtg aggagattag aaagaaaagt gatgtttaca taataatgtt gacagctctt
    31141 gatgaagagg tggatattgt cacaggatat gatttaggtg cagatgatta tatcacaaag
    31201 ccttttagct tgatggtatt aatatcaaaa gtgaatgcgc ttatgaaaag agtaaataca
    31261 gtaaaaaatt atactctatt agtttgtgat gatttattct tttattatat agaaaataaa
    31321 ttaattataa gaacagatga taaggaagaa gagataattt taagtaagac agagacaaaa
    31381 ctattaaaat atttgatgga aaattctatg cagacattga ctaaagagca actgttagaa
    31441 tctttatggg atagtagtgg taattttgta gatgataata cgattgcagt aaatataaga
    31501 agacttagac aaaaggttga aaaaaatcca tctgaaccta aatatataaa aacagtaaga
    31561 ggggttggat atatgtgggg agaaaggagt ataaaaaaat gctaaaaaat atgaacctca
    31621 aagatggttt aagtgtgaaa gatgattata taatgagaag agttattgta tattctgttt
    31681 tatctgtact aatagtaacc cttgtattta tactttttga atatagcaga ttacataaat
    31741 tgtatgaaca tcaaataaaa aatactcaaa atgttgctgg aatattgatt gaaaaatatc
    31801 ctaatgatga aatagatata gtaaattcta tttataattc tgattatagt aaggtgtcta
    31861 ctggagaaaa ggcatttaag aaatttggct atggattgga aaataaaatg tcaaatgata
    31921 aaaattttag cttatactta aaatcttttt ttaaggaatc attttttatt tttttaatga
    31981 tgtttatagt agttataggt ataatatatt attttataag atacataaat aaaagattga
    32041 gtaaaattta ttttatagta gataagatgt cacaagaaaa ctacttgaag gacactactg
    32101 agtcagaaga aactggttat ctagtagaat ataatgaata ctttaaagaa ggcatattta
    32161 gtaagataaa taactgtcta tatgagctta atagaagttt aaagataaag tttattaaat
    32221 tagaaaaaga aaaagaaagt gtaaaatcat tagtaacaga tatttcccat caattaaaaa
    32281 ctcctttagc atccttaaaa ttatacaata cattacttat agaagaagaa ttagaagaag
    32341 atgaagccaa cgagttctta ttaaccaata aggcttctat aaataaatta gaaaatttaa
    32401 taaattcttt agtgaacata tcaaggttag aaattagcat gataaacata aaaaaagaag
    32461 ataatgatat aaaaaatact attctaaatg cagtaaaaag tgttacccct aaggctaaga
    32521 gcaaaagtat aacattaaat ttaaatgaat ttaacagtag aattatacca catgataaaa
    32581 agtggactga ggagagtatt tttaatgtat tagacaatgc tgtaaagtat acacaggtaa
    32641 atggagaaat aaatatatct gtagaagaaa ctgctaattt ctttaaaatt ataatagagg
    32701 ataatggcat agggattgaa aaaagtgagt ttaataatat atttaaacgt ttttatagag
    32761 gttctgctga tgaaattgaa tctgtagaag gtagtggggt aggattgtat ttaagtagaa
    32821 agattttaga agaacaaggt ggaaatatta tagtttcttc aaaagtagga atagggagca
    32881 agttttcttt gtttcttaca actatgtaat attaaatgaa aggaaaatgt aagcctctac
    32941 atttattata taagtataaa cagctccaga aataaaaaat taataaaata tggagtaata
    33001 taacttagaa tgagaggata attttatgaa aatactatat actgaaaatt tatcaaagca
    33061 ttatggaaaa ggagaaagct tagtaagagc tttggacaat gttgacttag aaataaatga
    33121 aggtgaattt gttgcaataa taggaaaatc aggttctgga aaaagtacac ttttgcatat
    33181 gattggaggt cttgatatac ctacatctgg gaaggtgtat atagataata aaaatatatt
    33241 tacattaaaa gaagaagaac ttgctgtatt tagaagaaga aaaatagggt ttatatttca
    33301 aagctataat ttaattccat ctcttaatgt atgggaaaat gtagtgttgc caatcggttt
    33361 agatggtaga gaggtagatg aaccttttat aaaagagctt ttaaaatcat taggacttga
    33421 aaataaacac gacgttttac caaatacatt atcaggtggt caacaacaaa gggtggcaat
    33481 agcaagagca ttagctacaa gaccagctat aatacttgct gatgaaccaa ctggaaacct
    33541 agattctaaa acatctgatg aggttatgag tatattaaaa tctatgtcta agaagtacag
    33601 tcaaactcta gttatgataa ctcatgatga cagtatagcc caaatggctg atagagttat
    33661 attcatagaa gatggcaggg tgtcaaaggt tggtgataaa aatgattaat actactaaaa
    33721 tagcaaaaag taatttaaag caaaataaat caaaaagtat tctaataata attactataa
    33781 tactttcaac cttaatgctt tcttctattg gaatttatat tgttgatgca ggagcatatc
    33841 aaaaagaaaa tactataaag tattcaggta attatcaagg tatattagca aatgtagacg
    33901 aaaaacaagc tgatatattg agtaatcatg cagatgttga attaactggt aaaatgaatg
    33961 gagtaggtgt tgaaaaactg gaagatgatt caaatatttc tctagcatac atgaatgaag
    34021 atgctttgaa attaaatagt tttgaattta tcaaaggaaa attgccatca aaagagaatg
    34081 aaattgtgtt agatagtgga gctttaaagg cacttggtta taaaaataaa gatttaggtg
    34141 aaaagataaa aatatcttat aatgactata aaagtgataa aaaaattgaa aaagagttca
    34201 ttataagtgg aatcttaaaa actagcgaaa tatctgaagc aggtaaatat tattatgcta
    34261 ttatatcaga atcatatatg agaaatacta gagatatgtc acaagaagat tttaatatat
    34321 atgtgaaatt aaatgataaa agtaatttat cgatagaaca agcaaaatca aaattaaata
    34381 agattgctaa tgatgtagga ttggacacta ttaatgtagc tgtaaatgaa aactatataa
    34441 atgctttaaa accagatatg gaaacaatta tgggtggagt atttgtaggg ttagtaattg
    34501 tattgtctag tattttagta atatacaata tattttatat ttcaatagtt acaaaggttc
    34561 aagaatttgg aaagcttaga gcaattggtg caactaaaaa acaagtaaaa aatatagtat
    34621 ttaaggaagg gttcatatta gccggaatag caataccaat aggaataatt ttaggctatg
    34681 tacttgcaaa tattataata aaatcattta tggatataga tgtaaaatca tcacgattac
    34741 cagttatatt atcggttgtg gtaataagct ttatatctgt tgttttatca cttttaaaac
    34801 caatgaaagt agcatcaaaa gtatccattg ttgacgcagt aagatataca ggaaataaaa
    34861 taagtaataa gagtaagaga aaaggatata aaaatataaa tctaaagaga ttatcacatg
    34921 ccaatttaga aagaaataaa aaacgtactt acatgacatt ggcatcactt atacttagtg
    34981 gaactatatt tataacagta tcaactgcct tagaaagttt tgatgctgag aaaatggcaa
    35041 gagaacactt tccttatgat atagaagtta gactatctgg ttatgaaatg aacagtgata
    35101 aaaatcctaa agataatttg aatattttac aaatggataa tcctctaagt aaagattttt
    35161 ttaatcaaat aaagaacatg gaaggaatta aaagaataga atctgcaaga agtgtaaaaa
    35221 ttggtatgga ggactatgat gttgaattta agtatgacct tttacaaagt ataaatgaaa
    35281 atgatgtaaa atcattaaac aagaatataa tagatggaaa aataaatctt gaaagacttc
    35341 aaactggaga tgaaatagta attactcatg ttgatacagc aaaagaaatg ggagtaaaag
    35401 caggagataa aataagatta actttatatg atggagataa aaagataaaa aaagaattta
    35461 aagttcaagc aatagctatg ggggttccaa gttttggtat agggaaggat tttattgata
    35521 gaactttgaa atacgattca actagtagct tgggaatata tacagaagaa ggaaagtatc
    35581 aagaaatcaa agatagtatc aagaagattg ctaaaaataa tggattttta gagactgatt
    35641 ttatagatag tagaattgag tcaaataagg ctactatttc atttataaag ataatgggat
    35701 acacgctaac aggcataata ggtgtgattg gatttatgaa tcttgtcaac acaatgataa
    35761 ctagcatagt tactagaaaa aaagaacttg gtatgcttca agctattggt cttactaata
    35821 aacaattagt gaaaatgctt aattcagagg caatgtatta cacttctgga atgatgatag
    35881 gaagtattct atttggaggt atattagggt atattgctgt tatatttcta aaaaagactg
    35941 gattatctta tgcaacatat tctttgccaa tagtaccaat cttgttgatg attgtgtgta
    36001 ttcttatagc tcaatttata acaacttatt tgataggacg aagttttaat aaagaatctc
    36061 ttattgatag agtaagatac agtgaataaa atacaaatat ttatggttag tttaactagc
    36121 aaaaaacata taaaaaaata tagttaaaga gattcataat taaataggtt aaagcatagt
    36181 acagagcaaa caatttaagc agatgtttgc tctgtatttt attatgtaat caataatata
    36241 tctaatatac tgatattatt catgttagaa aatgtaccgg tacaattcta aaaaagtata
    36301 aaattgtacc atataaaatg aaaattttgt ataataatcg tatagaaaat aaataggagc
    36361 tgatatatat gaataagaag attaattatg caccaggacc aactgaaact agggaaaatg
    36421 taagattagc aagagcagaa aaaacaacaa atccagatat agatattgat tttgttgaat
    36481 tttataaaaa gacatgcgag aagtttggta atataattgg aacaaaaaac gatgtttaca
    36541 tattgagtgg tgaaggaata ttaggtcttg aagcagcatg tgcatcactt acagaaaaag
    36601 gagatagagt acttgtaatt gacaatggta tatatggaga aggttttaaa gattttgtaa
    36661 caatgtatgg tggggagtat gtgctttttt caagtgaata tactaaaagt atagatattg
    36721 atgaattgaa agcattttta gatgaggata gtaattttaa atatgcaaca gtagtgcatt
    36781 gtgatactcc aactggcgta ttaaatgatg taagcaaaat atgtccatta cttaaagaat
    36841 atggaatatt aactgtagtt gattctgtag caggtatggt aggagaaaga ttaagtgtag
    36901 atgaatctaa aatagatata atattaggtg gttcacaaaa agctatttct gcaccagcag
    36961 gacttactat agtcggaata agtcaagatg caaaaaattg tataaaaaat agaaaaactg
    37021 atgtaatagg tttttattgt aatttaaata tatgggaagg ctattatgaa aaaaaatact
    37081 tcccttatac aatgccaata agtgacataa tggggcttga taaagcactt gataatatat
    37141 tagaagaagg aatagaaaat gtattaaata gacatgaaaa gatagcttat agtgttagaa
    37201 aatctattga ggaatatggt ctagaactat ttttagaaga aggatattct aatacagtca
    37261 cagctataaa aattcctgaa agtataggag ctttagaact tactgattat atgcttaaaa
    37321 attataatac acttgttgca acatcactaa atcaatatat ggatactatt ttaagaatag
    37381 gtcatatggg agagaatgca aatctaaata aaatagaaca tgtactaaat gttttggata
    37441 aaagtttaag tgcattagga ttcaaaggaa atggaaattt gttaaattta tttaataagt
    37501 attattttta atagataggg ggatatagta tggaagttac agctaagcaa aaatttacaa
    37561 cgaaagacat ggtggaaacc tcactattaa tagcattagt atttattgca actaaattta
    37621 taaacataag attaccaata tctataaatg gtgggcttgt gcatttagga actgcaatgt
    37681 tgttcatatc agcaatagta tttggaagta aaaaaggtgc tttatcagga gctattggta
    37741 tgtcactttt tgatttgata tctgggtgga ctttatgggc accgtttacc tttatagtta
    37801 gaggagttat gggttattta cttggaaaga ttgcatgggc aaatggtaat aatgggaata
    37861 attttttaat caatgtaata ggaatatgtg tttcatcaat ttggatgcta tttggatatt
    37921 atgtaacaga agttatactt tatggtaatt ttatagtacc tcttacttca ataccaggta
    37981 atttaatgca agtattgata ggtcttataa tagcgcttcc tatttctaag gtattgaaaa
    38041 aatgtataaa ataaatataa agtgaactaa aatttatgat agcataaatt atactgtata
    38101 atattaggat ggagtacata attaaaaaat tttagtatag aggttagttc acaatgttat
    38161 cagttacaag atattcaatc aaaaatgttc gtttaaagcc gtgggtaaag tttatttggt
    38221 attttgagac aaaatcaaat atactgttaa ataataagtt acttccgaca gacagtatag
    38281 atattattct caatctttct gatgttatgg aatataaaat tgaaaatcaa gactatattg
    38341 ctagtaatat tcattttaat ggaataaggg ataaacatgg atttattatt caacatggaa
    38401 atatacgtgt tatagggatt tctttttatc catttggtct ctatccattt ttaaagatac
    38461 caatttcaga gtttaacaga caaattgttg acctagaagc agtatcaaaa ctatttgcaa
    38521 aaaaattaga agagtcatta aatccaacaa aagcaataga aaaagttgtt ttatgtttag
    38581 aagaagtatt attatccata ttagaagaag atttaatttc aaataaatat gtaaagcttc
    38641 taaattcatt tatatatgtt aatagatata gcaatataaa agcattttgt gatgatacaa
    38701 atataaatat taaaacattg gaaagaatct gtttaaaata tacagggtat acacctaaga
    38761 ttttaaagag aatttacaga tttaaaatgg caagcaatca acttatttat aattataaag
    38821 atgatgaact ttttgatttt atttatgaaa atgagtacta tgaccaagca cactttataa
    38881 aagaatttaa aaaattttca gggacgtcac caattaaatt tagtggtgag aataagacga
    38941 ttaaagaaaa tataaagtac agctatttat aattatactg tcgatttttt acaatacctc
    39001 caatttattt tttgatatga tataggaaaa taaattggag gttttacata tgaaaattat
    39061 tacagaaata gttgagttta atgtggaaga aaatttatca aaagaagagt ttatagaaat
    39121 tgtaaatgac ttagaattaa aatttcattc tattcaatct ggttttattg atactgagct
    39181 tttatatgat gaaaagtctc aaaagtggat tatgatacaa cattgggaat ctatagaaga
    39241 cctcaagagt gtttctagta aaatgtttaa agattcttca actgagaaat ttagaaaagc
    39301 tttaaagaat caaactgtta agatgaatat aataccacaa attaaaagtt ggaaattata
    39361 gaactgaatg taaaagatag ttaaatagta tcaaactaaa ttagccaaat aacattttgt
    39421 atttaaattg ttatttggct tttagtgttt attttaatat gagtgttata tactagaaaa
    39481 accaacaaac tgttagagtg tggattatca taatgtcata ttcgggagct aaaaggctta
    39541 atactgattt gccaattggt caatccatgc atgtccatct ggatatgctg ttattaaagc
    39601 atttacaagc caatcccttt gctcttgtga taatagtggg actgttacat cgaagtcttt
    39661 ttgataacca agcctatcta tgtatattga tttgcaaaac aaaaccactt cgggagccat
    39721 atatgggata ttatctgatg ttatcagtct ggccttatcc atatctctta taattctttg
    39781 attttgggaa aaaagaaatt ttccatcttt ctgtggatta tatatcaatt caataaaatc
    39841 gaactgtaac tgttcgtcac actgtatatt cattttatag ttatcatcct cgactttttt
    39901 taattcaata aatgagcaat caggcttaat cgcaaaaaca caaatttgct ctatatgttg
    39961 cttgttaagg tcgataatag gtcgtaataa ataattaccc aagggctcat atatatccca
    40021 tccttgatta agcatatact gaatgctctt ttctttatct gtgtcagaaa tccatatgtc
    40081 aatatcactg tgtgggcgaa tatgcatatt cgcaaataat tctagggcat atccaccaca
    40141 tattgaatat gaaaagccac aatcattaaa aaagtttaca caatctttaa ttagtttgtt
    40201 catagcaacc tccttttgca tgtactgaaa taaattaaat tttttaaaat accaactaaa
    40261 tgtttgtttt gtgtaaatat aaaactgccg atattaaggc agttttatat ttactattta
    40321 gaaaatttat taacaaattt ttgatattca cacattatct tatcattcat aaaagaaata
    40381 cccataaaag cagcttgctc accaaacaaa gtaaaacgtt ctatcatctg ttgtggatta
    40441 cctggataaa ctgttggaat acaccatgtg tttagaagaa aaccaaacat ttgagcagca
    40501 atctctggat ttttaacttc catagaatta tcttgatttc cttcttcaat caatgctttt
    40561 atccaaggca caatatcatt tataatctca tcaaactgcg tagctaaata cttaggatta
    40621 cgcaatagtg atgatgacat taaaaaaatg tcttgtttat tgtgctgttc ataagtatga
    40681 agcaacctat attgtaattt ttctaatgcg gtctttcctt catcattttt tacctcatac
    40741 ccataaaatc catcattata caaccgactt gtgattgcct taattatttc gtcttttgat
    40801 ttaaagtgat gataaatagc acctcttgaa aggccatcta aattatctac aatgtcttga
    40861 atcgttgtct gctcataccc tttttctaag aataacttat gtgaaatatc aagtattttt
    40921 tgtatagtat tatcaggttc tttttttctt cccaaaaaac tcactcctcg aaacaatcat
    40981 ttggtatgtt tatcttagca aatacataat gatttgtcaa tgtgtctttt tagcatgttg
    41041 aacaggtata ttgccatgct ttcattattt tatttttttg ccttatactg gactatattt
    41101 gcagtttcct aatatatttg gctcatattt aaataccatt atctaaaagc actattgctg
    41161 tacgagaggc aataggcatt ttagaagaag gaggagttga gctaaagtat acatcataat
    41221 ctcttaagtt ttcttttttt gtgtttcctc caaactctac catatcagta tttaatatcg
    41281 ttttgtagtt tgtattttga aactttgaaa tatcaactga ttgtattaga ttagggtgga
    41341 aattaaatat aaataaatat ttgcccttac tgtacaccaa aatcttttta tcattatcca
    41401 tatgtataag ttcatgacta ttttggataa aaatatcact gtattttgta agttcgagca
    41461 tagatttatc aaaatttaat aattgtttat actttaagtt gttatcttct gaaaggctcc
    41521 attgtcttct ggcatactta tagctccaat tgttgccttc tcgtggaaaa tcaatccact
    41581 caggatgtcc aaattcattt cccataaaat ttagatatcc ttcacctgct agagaaaaag
    41641 ttattaattt aattaattta ataagagata ttgctctatt gataacatgg ttatttgagt
    41701 ttatttccat attccagtac atttccttgt cagctagcca aaaaataatt gttttatcgc
    41761 caactaatgc ttggtcatgg gattcacaat atcctatatt tttttctcca ggtcgtctag
    41821 tagtaagttc ataccacatt ttgcccaaat cccagtcttc atcagataag ttagagatag
    41881 tttttatcca aaaatcaggt actcccattg cgagtctata gtcaaatcca attccgccgt
    41941 cttttatagg gatacacata ccaggcattc cactcatatc ttctgcaata cttatcgaat
    42001 taggtttaat ttctttcatt aattcatttg caaattggag atatgttata gcttcaatat
    42061 ctgtattcat actaaaatat ttttcataac tatcaaatga aactccaaga ccatggttat
    42121 gatataacat tgaagttaca ccatcaaacc taaagccatc aaaatggtac tcatttaacc
    42181 aaaattttat atttgaaagc aaaaaatgta ttacttcagg tttaccatag ttaaaaagtt
    42241 ttgtacccca agcaggatga tttccctttg aacctgagtg gaaaaattga tgttcacttc
    42301 catcaaactc atttattcct tctaaagtat tctttacagc atgagaatga actaaatcta
    42361 ataaaacagc aatccccatt gaatgagcag tgtttatgag gttttttaag tcttcaggag
    42421 taccaaacct agatgagatt gcataaaagt ttgaaacttg gtatccaaaa gatgcataat
    42481 atggatgttc cataatagcc ataagctgaa tggtattgta tccagctttt tttatcttag
    42541 gaagtatttt ttcagtaaat tcattataag ttccaataga ttcgctttca gtagacatac
    42601 ctatatgaca ttcatagatt agtggagaag ttatgttttt taagtcaaaa tcattgtcag
    42661 tccagataaa tggagttttt ggttgccata tttgtccatt aaatccacca aaatcttttt
    42721 gaattactct ttttatatat aatggtatac ggtcaaatgt ttttccattt gcagtaactt
    42781 gtacctttac ctctgatttg tgaggtaagc tattttgacc aggaataaat atttcccaat
    42841 taccagaccc aatttttctt agaggatgag attttctatt ccaattatta aagtcaccaa
    42901 ttagtgacaa actatctgca tttggagccc attccctata gaagaatcca tcatttgtct
    42961 gatgaaatcc atagtaaaga tgagcatttg caaaagaact aaaatcttta tagtttttca
    43021 atgtttttga tttcatatcc tcataatgtt tcattcgctt ttttatatca gattcaaatg
    43081 gttgtaggta tacatcaata tctaaaattt taaatctttt ttgacttttc attgtatttc
    43141 tcctccataa taaatattac atctaaaaaa taatatttat ctcatactat cttctctgta
    43201 taattgatat atattatata atcctaataa aaagcttaag ccaccaatga ttaatttcat
    43261 aatttacctc cctagtattt atacttatat atcgtattca tcaaaattta aaagtttgtt
    43321 taaaactctt ttaataatat cataatcaaa gcctttatag gaaagatgtt gagaaatttt
    43381 ttgatatatt ttctttttat cttctttctt tactctttca tacctttttt ttgctagata
    43441 catagcattt tcaaattcag tgtccttgtc taattcagat actgcttcat tgattgtact
    43501 tctattgata cctttattat ataaattctg cttaattctg tttttgccac atttatttag
    43561 gttaacattt gtgttaacaa ttttttgagc taatacactg tcatttacga gattataact
    43621 tttaagaaaa tctatgaccc tctcaattat attttcatca aattctgatg atagtttttc
    43681 ttttaatttt ttttcagatt ggtcagcatg agataaaata tttaatgctt tatttttagc
    43741 ttttatatac atttcatcat ctaaaatagg ttttaattct tcttcattta tttcattccc
    43801 tttttttaaa ttaaatgtat aaaccagttc tttaaatata gcaataaaaa atttatcatc
    43861 tacataaata ttaactctat catcatttct tttttgttgt tctatttttg taattatacc
    43921 cataagtcct ccttttttat atgtaattat attaacaaaa gcaactaata aaatttaaca
    43981 aaaaacttat atttgtatgt tatattttat caaaattaag acaaaatagt aaaaagttat
    44041 ttttattaaa aagtaaagca gaattatgtt aaataattat tttttaaagt gttttttagc
    44101 atataaatat aattatatac ccaaaaaaac aaattataag ctttaagaaa taaaaaataa
    44161 tggtacaata gtattataac attaaaagga gacaaaatat gagaagtgaa aatctagtta
    44221 agatggcaga gttgaaaaat actgaaaaac tagagaaatt taatcgccat aaaggcaaaa
    44281 tcaaaatagg tatattggga ggtacttttg accctataca ctatgctcat ttagcaacag
    44341 cagaatttat tagagacaag tatgacattg acaaaataat ctttatacca tcaggaaatc
    44401 caccacataa attatgtatc actacagata agtatgatag atataatatg acacttctag
    44461 caactgaaag caacgaagat tttttagttt caaaagtaga aatcgaaaga aagaaaagga
    44521 cttatacaat agatacatta aaatacttaa agaaaaaata caaaaatgca gacatttatt
    44581 ttataacagg tgcagatgca atttgtagtg ttgaagagtg gaaagatgta aagaaaaact
    44641 ttgagttagc tacttttata gccgcaacta gaccaggaat tagtttgtta aggtcacaag
    44701 agactataga aaaactaact aagaagtata atgctgacat tataacagtt tatgttccat
    44761 cactggatat atcgtccaca tatataaggg aacagctaaa tgaaggcaag tcaatacgtt
    44821 acttagtacc cgaaaatgta gaaaattatt tatatgaaaa taaactatat caatatggag
    44881 atgattaaat gaacttagaa aatattaacc aaagactaaa tcaaatgcta ccagtgggga
    44941 ggcttagcca ttcaaaaaat gttgcaaaat gtgctgaaaa attatgtgag atttatggat
    45001 gtgacaaaga aaaagcttac ttagcaggaa tgattcatga ttgtgcaaag tatttaagtg
    45061 acaaggaaat agaggattat gtaaataagt atgaaatata tttagaccca atagaggatg
    45121 gaaatcgttc cttgtctcat agtgtaattg gggcgtacat atgcgagtat gaatttgaag
    45181 tggaagatga agatattata aatgcaatca aatatcatac aacagggaga gaggacatgt
    45241 ctcttttaga aaaaataatt tacatagctg atttaattga agaaggaaga aaattcccag
    45301 ttgtagatac attaagagaa ttagcatatg gagggaagct tgatgaagca cttttaactt
    45361 cttttaataa tactttaatg tttgtcatca ataaaaaaga agagatacat ccaagaacag
    45421 ttatggcaag aaactatcta attaaagaaa agttattata aaaaagctat ataaaggcaa
    45481 acatattaat aaatttaaat ttgactatgt tacaaaatcg tattaatatg ttacaataac
    45541 taagtgtatt ttaattagga aagtgaggaa tttttatatg acagtggaac aaatgacaaa
    45601 aatagcatat gatgccatag aagataaact gggtcaagat acagttataa taaatatagg
    45661 aaaagtttct agtttgtgtg attattttat tattactaca gcatcttctc agagacaggt
    45721 aaaagctatt gctgacaatg tggaagatga attagcaaaa ctaggattag agccaagagg
    45781 taaagaagga caaggaactc aaacttgggt actccttgat tatggagaca taatggttca
    45841 tgtatttaat gaggaaaata gagggtttta taatttagaa aaattatgga aagatgcacc
    45901 ttatatagat attgacacat tagcataaac atgataatat atgttattat aattagaaaa
    45961 tttcaaaaat tatattaaat tgaatagact agagcagtaa aagattatat tttttcagag
    46021 agttagtgga aggtgagaac taatattata taactttgaa cttactagat taaaatctat
    46081 tctccatggc tggattaaaa gctatctaag agagtctata tatgtagtag gctaattagg
    46141 gtggtaccgc gaagatttat cctcgtccct aaacgtaagt ttagtgacga ggatttttta
    46201 ttttcaaaga attatttgga aattataagt aaataatact aaataaggta gttatacaca
    46261 attaaaaata ggaggcaaaa tatgagcgta tataatttta aagaagtaga atcgaaatgg
    46321 caaaaaatct ggaaagataa taatcagtat aaaatggata ctgcacaaac tgaaaaacct
    46381 aattattaca cattagaaat gttcccttat ccatctggaa aaatacacat gggacatgtt
    46441 agaaattatt ctataggtga tgttgtcgca agattcaaaa aaatggaagg atataatgta
    46501 cttcatccaa tgggatggga ttcttttgga ttaccagctg aaaatgcagc tataaaacat
    46561 ggaatacatc cacataaatg gacaatggaa aatatagaag aaatgaaaga gcaattaaat
    46621 ttattaggta ttagttatga ttgggataaa gaagttgcaa cttcaacgcc agaatactat
    46681 agatttacac aagaaatatt tttgaagttt ttagagcatg ggcttgcata taagaaaaaa
    46741 tcatatgtta actggtgtcc ttcttgtgag actgttcttg caaatgagca agttgttcaa
    46801 ggagcatgtg agagatgtaa agcaacagta ttaaagaaag atttagaaca atggtatttt
    46861 aagactactg aatttgcaga agaattactt aatgacttag atatattaga tggatggcca
    46921 gaaaaagtta aaacaatgca aaaaaattgg ataggtaaaa gtacaggagc agatttagtt
    46981 tttgatatag atggaactga taaatctatg acagtattta caactagacc agacacaact
    47041 tatggagtta cttatatggt acttgctcca gagcatgaat tagttaaaga attggttgca
    47101 gggactgaat atgaagcaga tgttgaagct tttgttcaaa aaatgcatac tatgacagaa
    47161 atagaaagaa cagcagctga tgtagaaaaa gaaggaatgt ttataggtag atatgttata
    47221 aatcctttaa atggcaagaa agttcctcta tggatagcta actatgtatt agttgagtat
    47281 ggaacaggtg ctataatggc agttccagca catgatgaaa gagatagaga ttttgctgaa
    47341 aaatataatt tagacataat tgatgtaata acagaagata acaaaatgat aaattcagaa
    47401 gaatttaatg gattagatgc ttcagaagga tttgaaggaa taatcgataa gttagaaaaa
    47461 gaaggtagag gaaagagaac aattaattat agacttagag actggttagt ttcaagacaa
    47521 agatactggg gttgtcctat acctgtggtt tattgtgatg aatgtggaat agttccagtt
    47581 aaaaaggaag atttaccagt tcttttacca acagatgttg aatttactgg aaaaggggaa
    47641 tctccactta cgacttcaaa gcaatttatg tcaacaactt gtcctcactg tggaaaacca
    47701 gctagaaggg aagttgatac tatggataca ttcgttgatt cttcttggta tttcttaaga
    47761 tatgtggata gcaataatga aaatgaacca tttagtaagg aattagttaa tagatggcat
    47821 ccagtagacc aatatatagg tggagtagaa catgctataa tgcatttact ttatgcaaga
    47881 tggtttgtaa aggcatttaa gagtatggga atggtagact tcaatgagcc atttaaaaac
    47941 ttacttactc aaggaatggt acttatggat ggttctaaaa tgagtaagtc taaagggaat
    48001 acagtatctc ctatggatat aatagatgag tatggagcag atactgcaag acttttcgta
    48061 ttatttgcag caccaccaga aagagatttg gactggtcag agcaaggtgt tgatggatgc
    48121 tttagattct taaatagggt ttatagattg gttgacgagt tagcagatgt ggttaaaaag
    48181 gatgtagagt ttggagaatt aaattcccaa gataaggata tgagatatac tatacactct
    48241 acacttaaaa aagttactgc tgatttaagt gaaaaattcg gatttaatac tgctatatca
    48301 gctcttatgg aattaataaa tgatatgtat aaatataaag agttagataa tataaacgaa
    48361 gcagttataa aagaaggtgt acaaactata gtaactataa ttgcaccatt tgcccctcat
    48421 ttaggtgaag aactatggac tatgataggt aaagaaggaa gtgtatttga catagactgg
    48481 cctaaatatg acgaaaaagc tttagtaaaa gatgaaatag aagttgttgt acaagttaat
    48541 ggaaaagtta gaggaaaatt aactgttaac tcaaatatat ctaaagatga gatggaaaag
    48601 gtagctttag aagatgaaaa aataaaagga ttagttgaag gtaaaactat agttaaagtt
    48661 gtagctgtac ctaaaaaact agtaaatata gttgttaaat aactcaaaaa tatgaacaaa
    48721 aatataaatt tataaaataa acgaggctgt ctcaaaatag agataagcct tttttcattg
    48781 ttgaaattat aaaattgttg aaattataaa aatagatttg attttataca aaaaaatgat
    48841 taaagaacta aaaagttcat tcttatatac cctagttgtt gttttaaata ttattataat
    48901 taatgttaaa tcgagttaat gtattaagtg gtatgtaaat cattcagttt ccaaagtgtt
    48961 gttcatcaag gaactgctct aagcttaaac gaaaaaaata taattattgt attttgaaat
    49021 ttttttctat ataatgaaaa caggcaaaaa aggtgcgtat ctatgcttta tatgaaacac
    49081 ccagacaacg aaattcagat tcatataata gctaccatgt ttctaaaaag gaacttgtaa
    49141 aaaagatagc gtttcaactt cccagttgat taaaattatc agcaggaaaa caagtatggt
    49201 cagcagtggt attctaatgg gtgttgtcga tatatccaag ttgggattgt ctgaggatgt
    49261 tatcaaataa cttgtgatag gtgcagtaga tgtggagatt cctgatacgg atttattctc
    49321 aggggatggt tgcttcggga gatatggagc gtaaccagtt tattgatatg gtaaccattt
    49381 tttgtctaat ttgatgaaag aatcccaaga ataccataca gaagtaagac aggaattaac
    49441 tggtggtata aactgataaa ggcaaattta aaaattttga taggatgatt ttaagattta
    49501 tcttctattt tgtgttttag aaataattga aatgttatga ggaggtatgt acaaatggag
    49561 actgtaacaa ataatcaatc aggtgtagaa attcaactca gtctgtttga agctatcaaa
    49621 gatttattgt taattaatcc tattttcttg ttgggaatta tcttgattgg tattttctta
    49681 gttgtaatat tcaagaaaaa tagtgatatt ccaaaggtaa aaacatctat tttatcactg
    49741 gcaatgtatt actacttatg tttaacacta tcgcatattg ttggtatccc tacattcagt
    49801 gaatatataa gactgtcaca gttaggggaa acgttcttta atccaaacat aaatcttatt
    49861 ccttttagtg atggatttag tttgagtttt attttgaata ttttactttt tgtcccatta
    49921 ggttttttat gtccgcttat cagcaaaacc tttgaacgtt tgagaaatac attcttgatt
    49981 ggttttggat tatctctttt tatcgaaact attcagcttt ttacgttaca cagagcaacc
    50041 gatatagacg acttactcac gaatgtaatt ggaacaataa taggctattt ctgcttcaaa
    50101 ctgattgcta aattgcgaat tgtaaaacta cattcagatg atgactctat ggaacaggat
    50161 tacacagtat gtattccgat tgccattata atagttgccc ttatattagg attttttagt
    50221 taagttccaa atagctaatt tgggaattat ggaggtcatt aagtgaataa aattactttt
    50281 atatgtttag gtgtaaaaag tatggagaag tcaattaaat tttatagaga tggattagga
    50341 ttttagatta caaactttta gtttaccaag gagatagcaa agttgtgtgc caaagggtaa
    50401 ccatttggaa atccagacaa cataacagat agtacgttac cttttacagg agcatactat
    50461 ctgttatatt gtcaacatcc tattttatgg tttgtgtgta gtttcctgta aactgaacta
    50521 aataattaaa tagaagctat tagaattaat aataacagat agatattaat aagcataaac
    50581 aaatagtaaa atgtgtagat atagacgttt ttaagtgaaa ctattataaa ataattgact
    50641 gataatcaac tgacaaaacc tattttttta ggtttaactg acatttaact gacagtcagt
    50701 ttttactatt taaatagaag ttcgcttaga ttgtcggaag cttctttgtc catttcttta
    50761 agtacatgag aatatctatt catagttatt tttatatctg tatgacccaa tctttcagaa
    50821 acaactttca tattagttcc ccctaaaagc agcagagtgg cacttgtata tcttaaatca
    50881 tgtattctaa tcttttttaa attatttttt ttgtaaaatt gtgaaacgtt tttcttagtg
    50941 aagaaggtag aaacggattt aaattggtgt ttagacaaac taaattattt ctgttttcca
    51001 ggatacctta caattttaat ttgctttgtc ttagtttttt aattttcaat ttttcaatta
    51061 attcttttgg agctgataaa gctctttttg attttggtgt ttttggctct ttaaaagata
    51121 ttttgccatt agaatagaca agaatttgat taacatttat cagattgtta tctaagtcaa
    51181 catctgacca cctaagccca gtagcttctc ccattcttaa acctagaagt aacattaaaa
    51241 gaataggtat ttctatatta ttactaagta acttttctat tagcaataat acttcttttc
    51301 tattgtatat ttcatttgta gaatattttt ctttagaagg aagttttata aaatcacatg
    51361 aattttcttg tatttctctt aatctatatg cttcccttat tacagaactt agaaagctat
    51421 accttacttt agcactagta aaggtagaat tattaaagtt ataatttaca aaagcttgta
    51481 ctgaactagg atttacttct gtgagtttta gattcttaaa aagaggttct atgtaatttt
    51541 taacccaaga ttttgtattt gtaacagtat atggagacca attattttta ttcatgtcca
    51601 tgtatttata acatctttct acaaatgtca tatcttttgg agcaacaaac ttattattgt
    51661 ttatagtaga ttttatttca attaaatgtt tttcagcatc ctttttcttt tcataacttc
    51721 cataactttt ctgttttatc ttgccagttg tttcctctat gtactccaca tatacataga
    51781 atttttcccc tctttttctt ataaaagctg atttgatatt catatatgta cgctcccttt
    51841 ataagttagt tttataaaag aacagttgta ctactctaat tatgtcatta tctttttaat
    51901 tctttacagt tacattataa cacgtatttg tatacactta aattaacagg aagataaata
    51961 tcgttaaagg aggaaaagaa aatttaatgt agaattatta agatatatag tatttcatat
    52021 ataaaagggg gagatattgt gaaattaaag aaattattat cactagggtt agtgtcaatg
    52081 tttgttgctg gttttcctat atcagctaat gcacttgata aaattgataa aattcaagga
    52141 gcagataaat acgaaactgc tggtatcata gctgacaaac aaagttatac tactgcaata
    52201 cttataaatg cagatagtac aatggctgat gggctaagtg cgagtggact tgcaggagca
    52261 acaaatgcac caatcctttt gactaaaaag aataatatac ctaatgcaac tcttaaaaga
    52321 gtagaaaaag ctaaaaaggt atacataata ggaggagaaa gttccattga taaagcaaca
    52381 gaaacttttc taaaagataa aggaatagag actagaaggc ttcaaggtaa tgataggatt
    52441 aagacaagtt ataatgtagc aaaagaaata aattccatta ataaagtaaa taaagtaata
    52501 ttaactaacg ctttcaaagg tgagccagat gctatgagtg ctgcacctgt agcagttaga
    52561 gataaagcag ctatagtatt aactgatgga aaaagtgttc catttaatac aactggtata
    52621 gaaagttatg caataggtgg cacttcatca atgagtgata gtttagtaaa tgatactaat
    52681 tcaactcgat tgggtggagt agatagatat gatactaata aaaaaatagt aaataagttc
    52741 tataatggcg caaaagaatt ttatatagct agtggtactg atttagttta tgctttagtt
    52801 gcttcaccaa tggctaaaaa taatcctatt gtactagttg atattggaag taataaagat
    52861 atacttaaaa atgctgcaaa agtaaccggg ataggaaatc taagtgataa aattatgcta
    52921 gagtgtgaaa ataccatcaa taatattagt cttccatctt caaataaaaa tataatatgt
    52981 ggattaaaga ttgattttga ttcaaatcac ataatttggc aagataaagg aagttatctt
    53041 gaatgttatg atgataacct tgaatattat actgaaaaat ataaagaccc aacagttcct
    53101 ataaatatag aaaatacaag tacacaaaat ttagatgtaa caaaattaaa agtttatgga
    53161 gaagctaatt caacattata tatggggcaa tacgctaaaa taaaaatgga ttttaatagt
    53221 ttcggtgtat taaagccagg agaaaaatgt actatttatt tagatataat ttcattagat
    53281 ccatcaagaa gcatgacatt aacattttct tatgtaggtg ataaagaaaa ttataaatat
    53341 gaacaaggat tggcaagtag aatattattt tataacccgg atggagaaga tttttttgaa
    53401 tataaataac tagtgggtat gtaaatgtct cgatattgct catatgttga gttattatat
    53461 ttagtttcat atgaactata tggattttaa aattaaaaat acttaaaaac atttatttta
    53521 atagtaggtg tttttttgat taaaagaata ttattataat gtaaaaagta ataaataggt
    53581 aaaatatgta agaattatat gttataatat ttttagcaag aagatgtaat ctacaattta
    53641 tagagtggag ttcatgtatc aaaaaattat cctcccgacg ttaagaaggg aggtgaatat
    53701 acatggataa ttttttacaa ggcatactag caagtctatc tgctagctta atagtttata
    53761 tagctagcaa actatttaga aagcgtaaaa aaccactcaa agcggcaact aagagtggtt
    53821 gggaatttga tttaaaaatc agattccata aaactaagta atttctaaat tatgaactcc
    53881 actctacagc aaaatagatt gtagttcttc ttgcttttat tatatcacaa attggtacag
    53941 atattcaaaa ataatatttt tatgatataa taaagtcata gaaattttgc agtgttcgat
    54001 tttttgaaga aattaaggct taacagttga aatataaggc attgaggact tgtgataagt
    54061 gttatcaatt gcactactca tggttcactg caaatttgag agatttgtat atgtgtaggc
    54121 attggaaata ctcaatttat tttggggttt tatattaact atatggaatg taaatttcat
    54181 attccatgca ttctgcttac acttaataga aaagttttat attaactata tggaatgtaa
    54241 attatttcta gcgacttggt cggttgctcc atttagcttg ttttatatta actatatgga
    54301 atgtaaattt tttcaaatct gatatatcga ccttaaattt cgtgggtttt atattaacta
    54361 tatggaatgt aaatgatata aactttggga gttcagaaga aggattaaaa agttttatat
    54421 taactatatg gaatgtaaat tagagatgaa ctcagataga aaaacatgta gatgtgtgtt
    54481 ttatattaac tatatggaat gtaaattctt caacagttgt gttaccatct cctacacgca
    54541 caggttttat attaactata tggaatgtaa attgctacgt aaccagtttc accgctttca
    54601 gtatataacg ttttatatta actatatgga atgtaaattg ttggtatagt ttcagaaaag
    54661 aaattcgaaa cacttgtttt atattaacta tatggaatgt aaattgtatc caaaaaatta
    54721 caataatcat acgaactgct aatataagca gtcaatatat gtgtacattt atctatttta
    54781 tctctaaata tataaattat tatcccccta aaaaactttc ataaaaacaa aaagaacaat
    54841 atctctttgg caactggctg acataactca taagatattt atataagtct tagtctctat
    54901 agctttgcgt cactagattt ctctagtttt gccgatttag ttttattcta taactaaata
    54961 atacaataga attagttatt attcaataag attgtttgaa aattgaataa atttataggt
    55021 atgaacgtaa tttcacactt aaaaaacaga ctaaatacgt agaatatata gaaaaattta
    55081 cctgtagttt ttgtgataaa aagctcctat tattaattta agctagataa gtttgaggaa
    55141 gtgacataaa ttgaaagaag gaatatatta tgttgaataa aaaattacca gatgcagaat
    55201 taaaaattat gaaatacata tagaatactg gttataaaac tgtaatatca aaagatgttg
    55261 cagatgaaat agaaaaaaca tatgcttgga agcatacaac tacaattaca cttttagcta
    55321 gattaactaa aaaaggtttt ttcatatcgc agagaattgg gaaacatgta cactatagag
    55381 tattaataaa agagaaagaa tatctcaaaa tagagggaaa aagaattttt ggaggtctac
    55441 ataacaatcc attatcagaa ttaatttcaa aattgcatga tggagaagaa ataactgaag
    55501 aaaaaataat agaaatagga aattggataa aaagctggaa agatgaagat taataaataa
    55561 aaggtagcta ctaatataag tgctaccttt tttcttgtgg ataatattgg ggataagttg
    55621 tggaaaactt gtggataaaa tattttgctg acatttaact gacataagta ttagattttg
    55681 atttaaaaat gttgaaatat caataaataa ttaaatagaa gttattagaa ttaacaatag
    55741 attccttttt caaagaaatc tttgatttca tcaaggttat cagtttttcc aagtgctaag
    55801 ataagtttta ttctagcttt ttgacctggt aagtccccac caaatataca accaagattt
    55861 tttaaatctc taccagaacc aaagtaacca taactatcga atactctacc tgaatgacat
    55921 ctagaaacta taactacagg aagtcctttt tctctagcat attcaactcc agaaagcatt
    55981 tgaggaggaa tattacctct acccattgcc tcaataacta tacctttata accactatca
    56041 acagcaaatc ttataaagtc agcattttct cctacatatg ctttaaataa ggcaacttta
    56101 gattctacct tatcagtatc tataattgtt ctatttacaa tatctcttgt aaggacaagt
    56161 tcgttgcagt caattattcc taatgggcca ctagttagag atttaaatgt atttagagaa
    56221 agagtatttg ttttagtagc ttcagaagct aataatactt cattgtttaa aactacaagg
    56281 actccttttc ccatagcatc tttggatact gcagtacaaa ctgatgcaga taaattactt
    56341 gaaccatcat atccaagttc agaactactt ctcatagcac ctgtgactat tacaggtttg
    56401 atgttgtcaa ttgtaaggtc tagaaaataa gctgtttcct ctaaactatc tgtaccatgt
    56461 gttattacaa cacctgtaat atcttttctt gctaataaat catttacata atttttcaat
    56521 tccatcattc tataaggtgt tatgtgaggt cctggaattt catcaaaatt aaaaacttca
    56581 acatctgcaa ctttatcaat gttggtaacc attgatagta tttgttcacc tgataaagtt
    56641 ggtatagctg ctccgacttt atcatctact gtcatagata tagttccacc agtaaaaact
    56701 atggcaactt ttttcttaat actcatacta ttctctgcct ttcataaata aaatttataa
    56761 ttccgatagg attatatcaa aaaaagtagt taaggtctac ataaactctt ttattcatat
    56821 aatttagata tttcagtgta atataattat ttcttaattt tttgccattc aacgatataa
    56881 ccatttaata tctctcttat ggcttttcct atcttagtat aagcttcatc atatagattt
    56941 gctagagata ttctaattga ccattcagga ccatagaatc catttccact aagtaaaact
    57001 actgaatagt cattagctaa tttataaaga acatctactg gagatgcagt tttacttata
    57061 aatttaaata agtcttctcc gtagtttaat ttagcccatt cttcaatatc aaattgagtg
    57121 taatatgcag cattgttttt gttttcatta attggaagct ctaaagcttc aaataatagt
    57181 ttttgacgtc tatggcagat actcatattg agttttttgt aggcatctac tttgtcaatc
    57241 aatgcaaaag cagagaaaaa cgccatttgt acttgctgtg gagtagaaag accagcagta
    57301 tggttgagag caacaagtct gctatcagca acaactcttt ccataaatga aaggctagaa
    57361 gggttaagac tcatatcact gtaacgctta tctactgatt tttttaattc tccagttaaa
    57421 tcatttattt tcttatcaaa aacatttttc ttatgaaggg caaatgtacc aagtctccat
    57481 cctgtaactc caaaatattt tgaataagaa tatactccaa cagtgttgta aggtagtttt
    57541 gacattaagg atgaaaaatc ttctacaaaa gttccatata catcatctga tattatcatt
    57601 aaatctttat tataattttc aattacatct attagattat tacaagatgt ttcatccata
    57661 gctatagaag caggattatt aggattaaca acgaataaag ctttaataga tttgtcacga
    57721 agtttctcta attctttttt tgtatattgc catgtatgag cacctttttc atcaacttca
    57781 ttagcattta tatttaccac tttaaagtcg taacgaggaa ggttaggtat ttcaagataa
    57841 ggtgtaaata ttggagtcat taaggctatg gtatctcctt tttttagaag ttcatttgcc
    57901 attaatgaat caaatatata gcacatagca gctgtagcac cttcaacagc aaaaatttct
    57961 acatcaccat atttacctga tatatcatat ttcatttctt gaactaggta atcgtgtact
    58021 attttttcca tatgaggtaa cattctatct ggaagtggat agttatcacc aataattcca
    58081 tcagctagtt catgaacaaa ttcatctcca tcaaatccaa gttcatttat accaaagttt
    58141 ataatatctc tagctaaata tgctccaggc atatctatat tttcatttat aaatttaaaa
    58201 aatctttctt taataccctt tttttgaggc attccagcca aatgacctaa atcccaatta
    58261 gaacgagttt cagtaatagc gaaatgacca aaagtaaaaa aggcttctct agcagttgaa
    58321 caagtccagt ttggatttcc acgacctgca tcaagtaatt ctctgttatt ttcttttgca
    58381 gtgtattttg caatatctat taatttgtcc ttaaattcaa aagggcttat ttttccatat
    58441 atgtttttga tttctttata agtatgattt ttcatgttaa attcctcctt atgtaataag
    58501 tttcacattt ttataataaa ataaacaagt tgcaaaaatg attatacttt aattataata
    58561 ggtttataga aaaagtaaag tatgaaatta aaataataac t
//
LOCUS       DAMJVK010000012        55087 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.012, whole genome shotgun sequence.
ACCESSION   DAMJVK010000012 DAMJVK010000000
VERSION     DAMJVK010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 55087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55087
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.012"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            359..1339
                     /locus_tag="PC414_000757"
     CDS             359..1339
                     /locus_tag="PC414_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF2463166.1"
                     /translation="MNLKLIWINILNPVFIKNEIKRKKIQIIIFFFINFGLTYSLGLL
                     LYYNKFIDPENFASFMMILPLSSVAIAKFYTEGLNNDMYEFYSLIILFFLIYSLLIVL
                     KLLNLINNNEFIIINLILTFISSLSIIIYSSNKKNLYPVKNIKLGSLLIIYFMISVIV
                     PTSFILILQGAQPNYSGIIKFIFTPITSLTTIYIFFSEEYAWRGFLQNIFFDKFGKKL
                     GVIILGMCWSLWHLPLIFTLYTPEAPILGIILRSIHIVGISIFLGYLYIKTKNIWFCA
                     IIHVLNNSAFLITNSKESTITYHDILIVSMVVLIFYVPFLFTKEYKNNLE"
     gene            complement(1438..2400)
                     /locus_tag="PC414_000758"
     CDS             complement(1438..2400)
                     /locus_tag="PC414_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF2463167.1"
                     /translation="MNKSWSNLIIKNKDKKVRSGWIIIAVMATFYILQYYLSMILVEI
                     IRKILVATGDINLKTNYFSEYANWLNDVGLQIVFQILTDSLMIIIPIIVWRFIMKNPI
                     SEMGLHSYRIKKKEGYVGMLLGIFNCTVIFIITITIGGGHVTSWVPKISPLTVSWILV
                     FILVAFAEEILNRGFFMAVLRRCKNIYFIMIVPSVIFGLIHIWNPDVTFLSVINIIII
                     GILFSYMFIKSSNIWMCIGYHFAWNVFQGIIYGMPVSGLQVPGIITTQITHGNLLNGG
                     MFGIEGGILTTFVNLLSFIFVWYYYRDSKYDFVSDKVNVTNIKN"
     gene            complement(2716..3666)
                     /gene="hpdA"
                     /locus_tag="PC414_000759"
     CDS             complement(2716..3666)
                     /gene="hpdA"
                     /locus_tag="PC414_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase activase"
                     /protein_id="HDF2463168.1"
                     /translation="MSSQKQLEGMIFDVQSFSVHDGPGCRTTVFLNGCPLSCKWCANP
                     ESWTVRPHMMFSELSCQYENGCTVCHGKCKNGALSFNLDNKPVIDWNICKDCESFECV
                     NSCYYNAFKLCAKPYTVDELVQVIKRDSNNWRSNGGVTFSGGEPLLQHEFLHEVLLKC
                     HEVNIHTAIETSACVSNEVFNKIFNDIDFAFIDIKHMDREKHKEQTGVYNDLILENIS
                     NLANSDWNGRLVLRVPVISGFNDSVENISDIISFMHKNNLVEINLLPFHRLGESKWIQ
                     LGKEYEYSDKGDVDEGHLEELQDIFLDNGIACYVGHETAF"
     gene            complement(3659..3916)
                     /gene="hpdC"
                     /locus_tag="PC414_000760"
     CDS             complement(3659..3916)
                     /gene="hpdC"
                     /locus_tag="PC414_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase small
                     subunit"
                     /protein_id="HDF2463169.1"
                     /translation="MRKHSDCMNFCAVDATKGICRLSKQMINLDDAACPEIKVMPKCK
                     NCKNFVEANDEGIGKCVGLEKEDWVYSTLNAITCEGHVFNE"
     gene            complement(3920..6628)
                     /gene="hpdB"
                     /locus_tag="PC414_000761"
     CDS             complement(3920..6628)
                     /gene="hpdB"
                     /locus_tag="PC414_000761"
                     /EC_number="4.1.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase large
                     subunit"
                     /protein_id="HDF2463170.1"
                     /translation="MSQSKEDKIRSILEAKNIKSNFQNKENLSEFNEKKASKRAEDLL
                     DVYYNTLSTADMEFPYWYNREYRKSDGDIPVVRRAKALKAAFSHMTPNIIPGEKIVMQ
                     KTRHYRGSFPMPWVSESFFVAQGEQMREEAKKLASNTADELTKFGSGGGNVTESFGNV
                     VSIAGKFGMRKEEVPVLVKMAKEWVGKSVEDLGFHYEKMMPDYDLKENLMSTLICMFD
                     SGYTLPQGREVINYFYPLNYGLDGIIEMAKECKKAVAGNASGDGLIGMDRLYFYEAVI
                     QVIEGLQTWILNYAKHAKYLESIETDLEAKKEYSDLVEILEHIAHKQPRTFREALQLT
                     YTIHIASVNEDAISGMSIGRFGQILYPWYEQDIEKGLITKEEVIELLELYRIKITCID
                     CFASAGVNGGVLSGNTFNTLSIGGLKEDGSTGANELEELLLEASMRCRTPQPSLTMLY
                     DEKLPEDFLMKAAECTKLGSGYPAWVNNSNGTTFMMKQFADEGMTVEEARAFALGGCL
                     ETSPGCWKQLTLNGKTYSIAGGAGQSAGSGVHFIANPKILELVLMNGKDYRMNIQVFE
                     PHNKPLDTYEEVIEVFKDYYKQAINVLERANNIELDIWRKFDTSIINSLLKPDCLDKG
                     QHIGNMGYRYNATLNVETCGTVTMVNSFAALKKLVYDDKAFTIEEMKDAILNNFGFKD
                     ALEVGNYSMADQVKVDKTGKYDAIYKACLDAPKYGNNDLYADNILKNYEVWLSKVCEE
                     AQSLYAKKMYPCQISVSTHGPQGAATLATPDGRLSGTTYSDGSVSAYAGTDKNGVYAL
                     FESATIWDQAVVQNSQMNLKLHPTTIKGQQGTKKLLDLTRSYLRKGGFHIQYNVVDSE
                     TLKDAQKNPDNYRQLMVRVAGFTQYWCELGKPIQDEVIARTEYEGV"
     gene            complement(6925..7941)
                     /gene="tsaD"
                     /locus_tag="PC414_000762"
     CDS             complement(6925..7941)
                     /gene="tsaD"
                     /locus_tag="PC414_000762"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDF2463171.1"
                     /translation="MSDIITLAIESSCDETAASVLKNGREVLSNIISTQIETHKKFGG
                     VVPEVASRKHVENIDIVVQEALDKANIGFNDIDHIAVTYGPGLVGALLVGLSYAKALA
                     YTLNIPLVGVNHIEGHLSANYIEHKDLKPPFITLIVSGGHTHLVEVKDYGKYEILGKT
                     RDDASGEAFDKISRAMNLGYPGGPIIDNLAKNGNKHAIEFPRAYLEEDSYDFSFSGLK
                     SSVLNYLNGKRMKNEEIVVEDVAASFQEAVVEVLSTKALKAVKDKGYNIITLSGGVAS
                     NSGLRAKITELAKDNGITVKYPPLILCTDNAAMIGCAGYYNFINGKTHDMSLNAVPNL
                     KINQ"
     gene            complement(7947..8423)
                     /gene="rimI"
                     /locus_tag="PC414_000763"
     CDS             complement(7947..8423)
                     /gene="rimI"
                     /locus_tag="PC414_000763"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDF2463172.1"
                     /translation="MEDNTKLIVDDIKIEEMTTEDIDEVFEVEKNCFEDYWSKESFRK
                     ELSNEVARYIVAKLNGKIVGYVGIWLILDEGHINNVAVHSDYRGKKIGDKLIKGIVDL
                     CKDNNIASMTLEVRASNKIAQNLYRKYGFKMGGIRKEYYSDNKEDAIIMWNQLKEV"
     gene            complement(8413..9129)
                     /gene="tsaB"
                     /locus_tag="PC414_000764"
     CDS             complement(8413..9129)
                     /gene="tsaB"
                     /locus_tag="PC414_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HDF2463173.1"
                     /translation="MKILGMDTSSMAASVAVVEDDNLICEFTVNNKKTHSQKLMPMIE
                     NMLSMSDLSIKDMDLLAVCIGPGSFTGLRIGMATVKAMAHVNNIPIIAVNSLESLANN
                     INFCDRKICCILDAQKNQVYSAKYKFENGEMIELDGVDVVEFDALVDEIVSTNEEFII
                     VGEAVYKYKDKLENIKNIKIPSPANNVSNAGSLCSLALNKYNKNIDVHTCYTINPMYI
                     RKSQAEVQYDEKMKRLNNGR"
     gene            complement(9129..9581)
                     /gene="tsaE"
                     /locus_tag="PC414_000765"
     CDS             complement(9129..9581)
                     /gene="tsaE"
                     /locus_tag="PC414_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDF2463174.1"
                     /translation="MAKIYLENENKTREIGYKLGKLLKEGSVICLVGDLGAGKTTMTQ
                     SLADSLGIEDYITSPTFTIINEYEGKIPLYHFDVYRIGSSDEMYDIGYDEYVNSNGIC
                     IIEWANLIEDILPKEYLNIELRYKDEGREMILTPKGEFYKEIVEELIK"
     gene            complement(9720..10760)
                     /locus_tag="PC414_000766"
     CDS             complement(9720..10760)
                     /locus_tag="PC414_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HDF2463175.1"
                     /translation="MHLSKKIKKLFLDDKGYYFNSNWKDIHIGIYDTKLGDSLKYITN
                     EYIENMERVKYDYRELKNIENQTDGMCILAIRQNYKALEYVKNQTEELCLEAIKYNYK
                     ALEYVKEQTEYLCLEAIKKDCNALKYVRNKTEGLIIKAISHSSNIDVVSILKALETQT
                     RRICLEAIKKDGRCLAYVREQSEELCIEAIKQNYKALKYVKNQTEKMCIESVRQNGMA
                     LQYVNKQTDKICIEAVKQDGRSIQFVNNKTEEICINAIRYLNKKYNIKDVLSYIDKYT
                     EDICIEIVRQNGKMLMYIKNQTEKMCIEAVKENYKSLKYVKEQSERICKEALKQNHKA
                     KEYVKIAIDDCI"
     gene            complement(11089..11694)
                     /locus_tag="PC414_000767"
     CDS             complement(11089..11694)
                     /locus_tag="PC414_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDF2463176.1"
                     /translation="MQNTAKRGNMISTRTLVVMSILSAISYLLMFIQAPIPGIFPDFL
                     KIDLSDIPAIFGGMSLGPLVGFGIVVVKNVLQAITATTTGGIGEFANILIGGSYVMIL
                     CYSYKRSGDLKGVLIGFVLGTISMTIIGSLVNYFVMLPLYGQLMGLDAIIGLGSAINP
                     QVHDLFTFVIWMIAPFNVLKAVIISLVTLPLYKKMGNIIKK"
     regulatory      complement(11819..11936)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(11996..13162)
                     /locus_tag="PC414_000768"
     CDS             complement(11996..13162)
                     /locus_tag="PC414_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2463177.1"
                     /translation="MIFIKNGKINTITNGIIHGDILIDEGKIIEIGEDLIAPLDVEVI
                     DASNKLVFPGFIDAHTHLGLWEDGIGFEGADGNEETDPITPQLNPIDGINPMDRTFKE
                     AFEGGITSVCTTPGSANVMGGQCIAIKTCGKRIDKMVIKNPVASKIAFGENPKSCYGQ
                     DDKSPQTRMAIAALLRENLKKAEEYLEDIDMYESHDDEDCEKPEYDIKMESLIPVLRR
                     EIPFKAHAHRADDMFTAIRIAKEFNLKLTLDHCTEGHLIVDELVEEGFPVIVGPSLSE
                     RSKFELRNLTFNTAGILSNAGLDVCIMTDHPVIPVQYLPICAGIAVKHGMKEEKAIES
                     ITINPAKALGIEDRVGSIEVGKDADLVIWDNSPLEIQSNVLYTIINGKVVYEKK"
     gene            complement(13310..15451)
                     /locus_tag="PC414_000769"
     CDS             complement(13310..15451)
                     /locus_tag="PC414_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDF2463178.1"
                     /translation="MDINQILKKEFNLRDEQINNTLKLIDEGNTIPFIARYRKEMTGE
                     MSDVTLREFYEKLMYLRNLQSRKDDVVRLIDEQGKLTDEITQNIEKAKTLQEVEDIYA
                     PYKQKKRTRATIAKEKGLENLALSILENNLDNIEIEAKNYLDEEKEVLSIEDALKGAR
                     DIIAELVSDDAKIRKYIRELALREGMIVSKSATDEKSVYDMYYDYSEAVKSMAPHRVL
                     AINRGEKESFLKVKLEINNDKVLNYIINEYVNDKNFKNKEEIVSSIEDSYKRLIFPSI
                     EREIRNHLTEIAQERAISVFGKNVKSLLLQPPVKDKVVMGFDPAFRTGCKIAVVDKNG
                     KLLDYTTVYPTDPQNDVEGAKKVLKGLIEKYDIDIISIGNGTASRESETFVSEMIKEI
                     DSEVQYVIVSEAGASVYSASELANEEHPDINVSIRGAISIARRLQDPLAELVKIDPKS
                     IGVGQYQHDLNKKRLEEVLDGVVEDSVNSVGVDLNTASYSLLEHVAGISKAIAKNIIA
                     YREENGDFTSRAQLKKVKRLGPQAFTQCAGFMRILEGKNPLDNTGVHPESYDICKKMI
                     EIIGYSLDDVKNKNIGEIDEKIKEIGLRELSEKLEVGQVTLKDIIAEIKKPGRDPREE
                     GIKPILRTDVLKIEDIQEGMTLKGTIRNVVDFGAFVDIGIKNDGLVHKSEMSNSFVKD
                     PMSIVTVGDIVDVKVIGIDLNKKRVALSMKK"
     gene            complement(15595..16702)
                     /gene="prfB"
                     /locus_tag="PC414_000770"
     CDS             complement(join(15595..16626,16628..16702))
                     /gene="prfB"
                     /locus_tag="PC414_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_153309863.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDF2463179.1"
                     /translation="MLKLQEYRHSMEQMASNLEELRVSLDIASLMKNIQINEEKMNQQ
                     DFWNDNEVAQRVLQENKSLKETLEEYESLKSLLEDIEVLIEIGLEEDDDSVERDIEKS
                     IESMEEKLSEMKIKTLLNGEYDKNNAILSINAGTGGLDAQDWAQMLLRMYIRWSESKG
                     YKVKLLDIISDPEAGIKTATILVEGTNAYGYLKSEKGVHRLVRISPFDPSGKRHTSFA
                     SIDVTPELDENIEVEINPSDLKIDTYRASGAGGQHVNTTDSAVRITHIPTGVVVQCQN
                     ERSQHLNKDRAMRLLMAKLIELKELEQKEKIEDIQGKYSQITWGSQIRSYVFQPYKLV
                     KDHRTNAEFGNVDSVMNGNIDLFINEYLKMNKIV"
     gene            complement(16717..19392)
                     /gene="secA"
                     /locus_tag="PC414_000771"
     CDS             complement(16717..19392)
                     /gene="secA"
                     /locus_tag="PC414_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDF2463180.1"
                     /translation="MSFMDNLFNMADKKELKKFNKTVDIIDSLEPKFESMADSELKNM
                     TNIFKERLANGESIDDILPEAFAVVREVSKRVLGLRHYRVQMIGGIVLHQGRIAEMKT
                     GEGKTLVATAPVYLNALTGKGVHVVTVNDYLAKRDRDQMAKIYEFLGMSVGVIIHGQN
                     PKVRKEQYDCDITYGTNNEYGFDYLKDNMVIHKEQRVQRGLNYAIVDEVDSILIDEAR
                     TPLIISGPGDKSTHLYSDANTFVLTLKPDDYELEEKDKAVSLTASGIQKAEVYFNVDN
                     ITDISHTELYHHINQALRAHVIMKKDVDYVAKDGEIVIVDEFTGRLMFGRRYSEGLHQ
                     AIEAKEGLKIQRESKTLATVTFQNYFRMYKKLSGMTGTAKTEEEEFKAIYKMDVFQVP
                     TNKLMIREDLPDCVYKSEIGKFNAVAQEIIERHKVNQPILVGTVSIEKSELLSQILKK
                     KGIKHEVLNAKHHDKEAEIIAQAGRLGAVTIATNMAGRGTDIVLGGNPDFLTKREMRR
                     NGFKEEIVNRVDTPIEGIPVKGNEILFEAREEYEKLFEKFKQQTQEEQKQVVEAGGLA
                     IIGTERHESRRIDNQLRGRAGRQGDPGSSRFYIGLDDDLMRLFGSDRISGIVDKIGLE
                     EDMPIEHRILSKSIEGAQKKVEGKNFGIRKHVLQYDDVMNKQREIIYAERKRVLEGED
                     LQEQIQSMTHSIIEEAVTLYTQDKGFDEEGFKEHMYNLFLPKGSIEIPEIEKLNPVEI
                     TEKVYEIAMKIYTSKEEQVGYERMREVERVILLQAVDNHWIDHIDAMDQLRQGIGLRA
                     VGQQDPVIAYKMEGFDMFDEMNKHIKEDTVRYLFNITIETPVERKAVVDVENLSSPSD
                     GTLPTSKTVKKDEKVGRNDLCPCGSGKKYKNCCGR"
     gene            complement(19524..20075)
                     /gene="raiA"
                     /locus_tag="PC414_000772"
     CDS             complement(19524..20075)
                     /gene="raiA"
                     /locus_tag="PC414_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDF2463181.1"
                     /translation="MNIIISGKQIELTDAIKNKIETKLSKLDNYIHPDTDVKVTVSAR
                     KARQKIEVTIATVNGPIIRAEDMQENLYTAIDVVYDKLSKQLKKYKKRLQDKHKDNKS
                     IRFQDAEINLADEQENYGDENQELVIQRRKKFSVKPMSEEEAVLQMELIGHDFYMFKN
                     IDSDEIAVVYKRNNGGYGIIEHE"
     gene            complement(20628..21302)
                     /locus_tag="PC414_000773"
     CDS             complement(20628..21302)
                     /locus_tag="PC414_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HDF2463182.1"
                     /translation="MLEIIGMSVEDAKIIEDCGADRIELVSALTEGGLTPSFGLIESV
                     VNSVKIPVNVMIRHHAKSFVYSKEDISIMQKDISVVKEIGANGVVFGVLDKNNSIDEK
                     NLNVLLKCCDNLDVTFHRAIDESNTIDSVKILKDYDKITNILTSGGKGSIVDNIQMIK
                     NMMLSSNHIKILLGGGLNFNNIEKIKELTKASNFHFGTAIRINNSPFEDIDRQKLKQL
                     VNIISR"
     gene            complement(21305..22357)
                     /locus_tag="PC414_000774"
     CDS             complement(21305..22357)
                     /locus_tag="PC414_000774"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HDF2463183.1"
                     /translation="MNIISNKIDELKEDLLSDIIDIVKIPSVKGESENGFPFGEKVGE
                     ALNKALEISEKLGFKVRNLDNYIGYAEHGDSDDYVCVIGHVDVVHEGDGWKHQPYKGE
                     ETNGRIYGRGVLDNKGPIMSALYGLYAIKELNLKLDKRVRIIFGTNEESGFEDIPYYL
                     EKEKAPIMGFTPDCKYPVVYGEKGMAKIRIKSKINYEEDVYLGFIENMSENVLVTYKE
                     LNIENSDTILDIKVKYDFSYKLKDVLDEIKASFPNSIDIEVISNFNPVYFDKESNLVK
                     KLQLAYERVTSLDGTPVTTNGGTYAKVMPNIVPFGPSFPGQKGIAHNPDEYMDIEDII
                     LNAKIFANAIYELAKE"
     gene            complement(22406..23365)
                     /locus_tag="PC414_000775"
     CDS             complement(22406..23365)
                     /locus_tag="PC414_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HDF2463184.1"
                     /translation="MWGMIATWRMALEGITEASEHLEKGMEAGDAVELAVRRVEDYPF
                     YKSVGYGGLPNENCEVELDAAFMDGNTLDIGAVAGIRDYSNPVSIARKLSYEKVNNFL
                     VGIGAEDYAHKNGFERKNMLTDRAKLHYKKRKKETLDKGLSPYAGHDTVGMISLDKTG
                     KMCAATSTSGLFMKKRGRIGDSALSGSGFYVDSSIGGATATGLGEDLMKGCISYEIVR
                     LMKEGKHPQEACDIAVNELDKQLIKRRGKAGDLSLVAMNNKGEWGVATNIADFTFSVV
                     TKEESPTVYISNRVDGKTIHKVISNEWMEAYEARIKAPIEVSE"
     gene            complement(23458..23979)
                     /locus_tag="PC414_000776"
     CDS             complement(23458..23979)
                     /locus_tag="PC414_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HDF2463185.1"
                     /translation="MKKIVMILDQIQAGAGGKEKSNIPPAGKSSPLGPGVMMEQFLNE
                     SKVIATLFCGDEFFVNNQEEVTSKMIAMVKKLNPDVVICGPSFNYENFSKMSAILSKN
                     INDKTDIPAFAAMSEENIDVINEYKNDICIVKTPKKGGIGLNDSLNNICKLAKAIANK
                     EDITLMKEEFCYQ"
     gene            complement(23990..25324)
                     /gene="celB"
                     /locus_tag="PC414_000777"
     CDS             complement(23990..25324)
                     /gene="celB"
                     /locus_tag="PC414_000777"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HDF2463186.1"
                     /translation="MNRLERILMPLAEKIGKNKLLIAIRDGFLVSSPLLIIGSLFLLV
                     ANFPIKGWNEFVGQIFGPDWAVKLQQPTVATFEVMTLLAVLGIGYSYAKQIDVDPIAS
                     AAVAIVAFFIITPFVIPYTPEGTESVYMVTGIPLGWMGSKGMFVGMITAITSVKLFAA
                     VVKKGWTLKMPDGVPPTVSKSFAALIPSAIVMVVFFLVKIAFEATPYGSIHEFIFKFL
                     QMPLLKLGNSLGAMAIAYIFLHLFWFFGINGSSVVGAVYNPILKILSAENLAAFQAGS
                     KIPNIITGQFQDMFATFGGAGSTLSLVIAMLLICKSKRIKSMGKLSILPGIFGINEPL
                     IFGLPIMLNPILLIPFAIVPTINIIIAYFCMSAGLVPLTNGVQLPWTTPIIFSGFLTT
                     GWQGAVLQLVLLILGIFMYIPFIKMLDKQYLREENETVEEEDDDISFDDLVL"
     gene            complement(25363..25689)
                     /locus_tag="PC414_000778"
     CDS             complement(25363..25689)
                     /locus_tag="PC414_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2463187.1"
                     /translation="MKRKVYLFCSFGMSTSLLADKMQKVADEHNLPIEVEAFPIAEID
                     KIVEEKNPDCILLGPQVKYMLKELKPKFEAQGKLIDIINEVDYGTMNGEKVLKLAIKL
                     IKSKQA"
     gene            complement(25713..26021)
                     /locus_tag="PC414_000779"
     CDS             complement(25713..26021)
                     /locus_tag="PC414_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF2463188.1"
                     /translation="MEEIVLKIIIHAGNAKSMLYEALDYAKENDFKKADELIENANEE
                     ILKAHKVQTELIQKEAGGDKSDISILLIHSQDHLMTCMSERNLIKEMIMLRKEIQKLK
                     "
     gene            complement(26162..28075)
                     /locus_tag="PC414_000780"
     CDS             complement(26162..28075)
                     /locus_tag="PC414_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2463189.1"
                     /translation="MICLNKRQENLLTTLLLKEEFINVNKIANDFECSERTIRNDCKY
                     IDEWLSTFCNAYIKRKPNIGIKFSGDGTDKILVNQKLRGEVKRVNSELHKQVEILNLL
                     LCNNKKVTLNDLSNKLYINKNIVREEISKLSVALNNYNLNISTKKGSGIYIEGDEKDI
                     RIMLVSFLFKYIDKYRLNIDEIEYFHIVDIIIVKNIMLTIEEDMDIRLTDISFKQLML
                     FLLINIIRIRLGNSLKVSNTKKELKLKMLILDIEDELKSNLSIVLNYEEKLFIESLIL
                     GMNKQINSNNIKKSLYLNEELASYTKKIINLVSEESGINFNNDILLYEQLICHLNVTM
                     HQMKSNVYLENPLLDNIKTKFCFLFTVISSSIEIELKGKQITEDEIGYLTLHFQTSLE
                     RKYSKRESNKKASIVCPFSFGVSMLLKVKIEKRFDNIKIIETLREEDLKRDNFNKTID
                     FIIAFQEYENIQKPIFITTPLFTDEDENKLKEFTNKIKEKDTSYKLMNRLMMSDYLIR
                     ELEHDDIYEAITYLTNLLIEKHYAEKEYLQSIITREKSYPTNVGKGILFPHGDMKYIK
                     QSVLCFARLKTPIKIRNGKDIKIILLLAYKKDDDRKVFRELFKEISNLTEDENMLDIL
                     SKCDIEKVKDILI"
     gene            complement(28407..29189)
                     /locus_tag="PC414_000781"
     CDS             complement(28407..29189)
                     /locus_tag="PC414_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HDF2463190.1"
                     /translation="MKICNDNFIKNFKNMINKCLDFIYPENISCIICDKSIKKTNTYS
                     ICKSCFKEMNFIQDGCMKCGKPIIRHSIEKEFIEECSYCFNKDFYFDKSISCIEYNDV
                     TKKMVLGLKYNQKTFMAKYIAQIMKEKLYLENIKFDYILFVPLHKKRLNKRGFNQAQK
                     IAFNLSKIVNIPLLDCISRKKYTRMLYKLNKKERKEELKNVFVVKENVKLINNKNILL
                     IDDIFTTGLTTNEISKLLKLSGANKVFVLTLLTKANDNYIME"
     gene            complement(29192..31411)
                     /locus_tag="PC414_000782"
     CDS             complement(29192..31411)
                     /locus_tag="PC414_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HDF2463191.1"
                     /translation="MEKLEGMISEIVFKNEDNGYTIAHLVNENDEIVVVGCMPTLAIG
                     ESIEVEGKWVNHKIYGTQFEVNSFMPVTPSSLEGIYVYLSSGMIHGIGEKMAKRIIDK
                     FGVDTLEVIQNSPEKLQEVEGIGSKKVKQIVKSYEEDRELRNIIIQLSPFGITPNYCL
                     KIYKKYKSSAIEVINKNPYQLAEDIRGIGFKVADSIASKIGIDKNSKDRICQGILYTL
                     NKSLSNGHTYLPEHVLIQDSEKLLELNGEIIKECVMMLVYNQKIHIEKVNNENLIYLM
                     PYYLAENGVCSQIVKLSQYEFEDLKIDIDNEINVLEEDKKIKLAEKQILAVKESVNSG
                     VLIITGGPGTGKTTTINAIIDIFENNGKSVTLAAPTGRAAKRMSETSNKEAKTIHRLL
                     EMGFSTDDDLTFFKDEEDPINSDVIIVDEVSMVDIILMYNLLRAIKLGTRVILVGDSD
                     QLPSVGAGNVLKDMINSNIINVVKLNEIFRQAQESMIIVNAHKINNGEPLYLNTKGKD
                     FFFIRKSTNEEILNEIIGLVNERLPKFYKVDKLKDIQVLSSMRKGELGVTNLNIELQK
                     YLNKKEKFKVEENFSKRLFRVGDKVMQVKNNYTKKWETEDQKESGEGIYNGDIGYVYH
                     IDKDKKTIYILFDQTKIVSYLYDELDEIDHSFCTTIHKSQGSEFPVVVLPIAWAPPML
                     LSRNLLYTAVTRAKKLVVLVGDVKYLEYMIKNNRVNQRYSNLGYKLNKFKQEGLLIE"
     gene            complement(31675..32772)
                     /locus_tag="PC414_000783"
     CDS             complement(31675..32772)
                     /locus_tag="PC414_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463192.1"
                     /translation="MSGKVNIKTVISFAGAYVATVIGSGFATGQEILQFFTFYGYAGI
                     IGGIISMVLFSWFGAEVIDKGRELKLKEPIKIYQVYCGKYLGTFFEWFGPLFLFGTLV
                     IMIAGAGATLSEYYGLNPYVGRIGMAIVSLITVSLGLTRLSKILGNIGPIIIIFTLLV
                     GAISLFSNIDALSNAGNMVDSLNIKTATSNGYFSGVLYTCYNVIIVITFLTGMGASAV
                     NKKDAVWGGIVGGVALMAAAIMMNLALLSDIGNIYTKEIPALYLADKISPIIGILFSV
                     VLLLGIYTTAVPLLWSVTNRFVEDDHPKFKIITIVVSILACIGGLLPFDKLVGTLYPY
                     TGYMGILILLCMLYRRITKTEGYKENKSEIS"
     gene            complement(33087..34280)
                     /locus_tag="PC414_000784"
     CDS             complement(33087..34280)
                     /locus_tag="PC414_000784"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDF2463193.1"
                     /translation="MARHLFTSESVTEGHPDKICDQISDSILDALLEKDPQSRVACET
                     TVTTGLVLVAGEISTSAYVDIPKLVRETVREIGYTRAKYGFDCDTCAVITSIDEQSGD
                     IAMGVDEGLESKTGEEIEEEIEKVGAGDQGIMFGFACNETPELMPLPISLAHKLSRRL
                     TEVRKTGLVDYLRPDGKTQVTVEYEGSKAVRVHTVLISAQHCETVSNDKIREDLINHV
                     IKEVIPAELLDEETKIYINPTGRFVIGGPQGDTGLTGRKIIIDTYGGYSRHGGGAFSG
                     KDPTKVDRSAAYAARYVAKNIVAAGLADKCEIELAYAIGIARPLSIFIDTFGTGKVSE
                     EKLVELVNKHFDLRPGAIIRDLDLRKPLYKKVAAYGHFGRTDIDLPWERTDKVEQLRK
                     DALGE"
     regulatory      complement(34417..34520)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            34709..35218
                     /gene="yyaC"
                     /locus_tag="PC414_000785"
     CDS             34709..35218
                     /gene="yyaC"
                     /locus_tag="PC414_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HDF2463194.1"
                     /translation="MYLAKVNYSDDDVIQMLSSVLKTIINENTIVVCIGTDRAIGDTL
                     GPLVGTILKNSNFKYPVYGTLDNPIHALNIYESLDTIKNTHIQGNFLAIDACLGSQSN
                     IGNIQIREGPILPGKGVGKKLPQIGNYSIVGIVDKIDENNKLSFNNIRLSFILDLAET
                     IALALLVST"
     gene            complement(35329..35757)
                     /gene="fabZ"
                     /locus_tag="PC414_000786"
     CDS             complement(35329..35757)
                     /gene="fabZ"
                     /locus_tag="PC414_000786"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDF2463195.1"
                     /translation="MLNIDEIKKLIPHRYPFLLVDKITELEVGKRAVGIKNITVNEPF
                     FQGHFPEYPLMPGVLIVEALAQVCGVAMMSVEENKGKLGVFAGIDKVRIKREVRPGDT
                     LTMEVEMTTLRKNIAKADAKAYVGEELVCKGELMFALVEK"
     gene            complement(35773..36792)
                     /locus_tag="PC414_000787"
     CDS             complement(35773..36792)
                     /locus_tag="PC414_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HDF2463196.1"
                     /translation="MAGADIGIDLGTANVLVYVSGKGIVLEEPSVVAIDKRTDSVLAV
                     GEEAKKMIGRTPGNIVAIRPLRDGVISDYDVTEKMLKSFIDKIVDKKGFGRFFMPRIM
                     VCVPTGVTEVEKRAVEDATRQAGAREVYIIEEPIAAAIGAGVDISQPNGNMIIDIGGG
                     TVDIAVISLGGAVVSESIKVGGDRFDDAIVKYMKKQHNLLIGERTAEKIKFEIGSAFK
                     REEEKYMKITGRNLITGLPSSITINSTEMLEALRECVEQIVVATHAVLEKTPPELAAD
                     IGDVGIIMTGGGSLLYGLDKIIEQRTGITVTIADEPLSCVAKGTGIALGSIDLLETGG
                     SFKRK"
     gene            complement(36989..37249)
                     /gene="spoIIID"
                     /locus_tag="PC414_000788"
     CDS             complement(36989..37249)
                     /gene="spoIIID"
                     /locus_tag="PC414_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HDF2463197.1"
                     /translation="MRSHIEERAIVVAKYILEKNTTVRQTAKTFGVSKSTIHKDVTER
                     LKEINPSLAKEVKNVLDKNKSERHIRGGLATKLKYEKEHKKM"
     gene            complement(37347..38015)
                     /locus_tag="PC414_000789"
     CDS             complement(37347..38015)
                     /locus_tag="PC414_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HDF2463198.1"
                     /translation="MKKKLLEKDGFYLSLFVCVCLLAVGGVWFTNNNVDKLASNKGIM
                     ENANKDSEEEIHLIEKDKKDAIPTATDSKQNLEKAKSKEENKSSTTKLNYIGDKVIRG
                     YSEKEPSYSKTLDVWETHKGVDISCTKGKEVKSLLNGIVVDVFDDEEYGQSVKIKSDN
                     NIVVVYSNLDKNVSVKKEQKVTEGQSLGTVGSTSQIESEEGIHVHLEAYSGEKSIDPM
                     SLIK"
     gene            complement(38081..39145)
                     /gene="spoIID"
                     /locus_tag="PC414_000790"
     CDS             complement(38081..39145)
                     /gene="spoIID"
                     /locus_tag="PC414_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HDF2463199.1"
                     /translation="MKNPLVVLLGFVTCSVLVPSLITLVSYKNVELTEKPESPVSINK
                     TIKKSDIEDKGNKEEKNTVNYETVNKKAPIINVYNHITGKTEKMDMENYLCGVLAGEM
                     SSEFDIEALKAQSVAARTYVVYKQEHGKSSKHKNAVVCTDYKHCQEYKSYDTLKKLNG
                     EEWIKNKYSKIQEAVRGTKGQIITYNDKAILPLYFSTSSGKTENSEEVFSAKYPYLKS
                     VESPYDKYSPKFASTLKISNTDFVKSLRRAYSTIVIDVNNLSKQVSITKRSDAGTVEK
                     IKLGNKELTGKDIRTVFKLNSANFDIKFGEGYIDFVVKGYGHGVGMSQWGAEGMAEEG
                     YKYYDILSHYYTDTKIKDIY"
     gene            complement(39232..40485)
                     /gene="murA"
                     /locus_tag="PC414_000791"
     CDS             complement(39232..40485)
                     /gene="murA"
                     /locus_tag="PC414_000791"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDF2463200.1"
                     /translation="MAKIIVKKSNPLKGSVKIDGAKNAVLPIIAATLLANGKSTLNGV
                     PNLRDVHVISDLLRHVGAEVEYKENTLTVDASNIKTCEAPYELVRKMRASFLVMGPLL
                     ARFNSTKISMPGGCAIGTRPIDLHLKGFKALGAKIEMDHGFVEAATEKLVGNKLYLDF
                     PSVGATENIMMAASLAEGTTIIENAAEEPEIVDLANFLNEMGADVKGAGTNTIKIKGV
                     KELKGAEHNVIPDRIEAATYMVAAAMTKGDITVENVLMEHLKPVVAKLREAGCEITEM
                     DNSVRVVGPKVLKPIDIKTLPHPGFPTDVQAQFMAMLTVANGTGVVIETVFENRFMHV
                     AEFNRMGANIKIDGRSAVVNGVDELHGAAVNATDLRAGAALILCGLIAEGETQIGEIY
                     HIQRGYVDIDKKITALGGQIEIVED"
     gene            complement(40506..41243)
                     /locus_tag="PC414_000792"
     CDS             complement(40506..41243)
                     /locus_tag="PC414_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwmB family TATA-box binding protein"
                     /protein_id="HDF2463201.1"
                     /translation="MKKVKLAISFFILMIIGMTISYADTSINTRYNRLSKALEISEAN
                     FKFYNVKINSVINHNLSNKEFHNIFIEIINNLKLDKKGINWNQKGSGDKIQLCATIKG
                     SDKEISIKGINKNNKESYIVVDILDNKVYKHKEVIYSNVENVLNKYSSQVDVSACIVG
                     EYTKRLQNNKYDDILQKILYNMSAKQIDEVKDVNFLSITAYSKVLNDYELEYLGEKIN
                     LNIGIKYNEDDGKTLIYIATPIIKLDY"
     gene            complement(41699..42994)
                     /locus_tag="PC414_000793"
     CDS             complement(41699..42994)
                     /locus_tag="PC414_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase"
                     /protein_id="HDF2463202.1"
                     /translation="MTNDFKKEKKEDYFVNLKAISTEVLQEKVQDNAWVIVDTRLNDA
                     YNGWKLDGVKRGGHIKGAVDFSANWLSVYSDRKDEVLEQALKTKRIDLDKNIVLYDAN
                     GKDALVVADYLSKKGYKYLYKYDIKQWADDENLPMERYKNYQMIVPAFIIKDILDGKI
                     PETFEDSKNIKMIEASWREESYTKGHIPTSVHVNTDIIEPPPTWMLDNDDNLTKFALD
                     YGLTKDDTVIVSSSTPMASYRLAVILRYIGVKDVRVLNGGTNSWLSAGYELEFISNPK
                     HSCTNFGADIPVNSQLIVTTSELRQKLKEKNKFILVDNRTWDEHIGKVSGYTYYDKKG
                     RIPGALYGHSGSDSVSLEEYRNIDNTMRNKYEILEMWDKENIDVNKQLIFMCGSGWRA
                     AEVLTYANVIGVENTSLYSDGWMGWSLDNSNLIEVGEHK"
     gene            complement(43445..45277)
                     /gene="glmS"
                     /locus_tag="PC414_000794"
     CDS             complement(43445..45277)
                     /gene="glmS"
                     /locus_tag="PC414_000794"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003401499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HDF2463203.1"
                     /translation="MCGIVGYLGSRKAAEVIVEGLSKLEYRGYDSAGVAVNSSNEKEL
                     NIRKFKGRLSVLAEDLEKNPIDGNLGIGHTRWATHGEPSDVNSHPHFNQAKTIAVVHN
                     GIIENYMEIKEELISEGVKFESQTDTEVIAHLVDKYYEGNLLDAVYKTISKLRGAYAL
                     GVICKEHGNELVAVRKDSPLVVGVGEGENFIASDIPALLKYTRDVYFLENGEVVHLKD
                     ENVTVYDSNRNLVEKEVFHVTWDVEAASKGGYDYFMSKEIHEQPTGVRETLERRLDDN
                     GNIILDSINISKEDLEKINKVYIVACGTAYNAGLLGKYAIEKFVNIPVITDIASEFRY
                     SDPFVDENSLVILVSQSGETADTLAVLRDSKAKGARILSITNVVGSSIARESDDVFYT
                     WAGPEVAVASTKAYTTQITSLYMIALDFAIKKGTITREFYDSMISKMKEIPSKIQEIL
                     DNEEYIKEVAKTVVSSEHAFYLGRGIDYSLAMEGSLKLKEISYIHAEAFAAGELKHGT
                     IALIEKGTPVIAIATQEKLFEKMVSNMEEVRARGAYVVAIAQSHNKDVEKAADKIIYI
                     PNSDDILSPILAVVPMQLLAYHVSVLRGCDVDKPRNLAKSVTVE"
     gene            complement(45544..46890)
                     /locus_tag="PC414_000795"
     CDS             complement(45544..46890)
                     /locus_tag="PC414_000795"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HDF2463204.1"
                     /translation="MRKYFGTDGVRGVANTELTCDLAYKLGRAGGFVLAQGDHRVKVV
                     VGKDTRISGDMLEASLIAGLMSVGCDVITVGIIPTPAVAYLTRKYGADCGVVISASHN
                     PVEYNGIKFFNKNGYKLDDEIELKIEEYIDDIDKIDCLPIGENVGRKLHEHCAQRDYV
                     DYLKSIISTDFKGLKVVLDCANGASYKVAPIVFDELGASVISINSSPDGNNINYKCGS
                     THPEQLQRAVLEHNADLGLAYDGDADRLIAVNEKGQIVDGDHIMILSALNLKKNNKLA
                     QDTLVVTVMSNIGLTIAAKENGINLSTTAVGDRYVLEDMVKNGYNLGGEQSGHMIFLD
                     YNTTGDGVLSSLILANIILQEKKPLSEIASIMSQYPQVLVNATIKNENKNKYMEYPEI
                     KTEIERIESILDGNGRVLIRPSGTEPLVRVMLEGKEEGQIKELATNLANLIQEKLS"
     gene            complement(47060..47617)
                     /locus_tag="PC414_000796"
     CDS             complement(47060..47617)
                     /locus_tag="PC414_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HDF2463205.1"
                     /translation="MSTARVICAGFGGQGVMSMGQLLTYAGMLEGKEVSWLPSYGPEM
                     RGGTANCAVTVSNEPVGSPLITDDATAAILLNIPAFEKFKDDVIPGGKIIVNSSLIKE
                     KVDRTDVDVYYIPANELAAELGNDKVANMIMLGAYLKVSDTVDIESVLEAFKKVFGPR
                     KEKFVPLNREALQKGMDATCSKATN"
     gene            complement(47618..48370)
                     /locus_tag="PC414_000797"
     CDS             complement(47618..48370)
                     /locus_tag="PC414_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HDF2463206.1"
                     /translation="MAVVFKKTEGLQDTQTHYCPGCTHGIIHRLVGEVLEELGVLGDA
                     VGVVPVGCSVLGYKYFNCDTQEAAHGRAPAAATGIKRVHPENTVFTYQGDGDLASIGT
                     AEIVHAAARGEKITTIFVNNTTYGMTGGQMAPTTLVGQRATTAQSGRNAETQGYPIRV
                     SEMLATLTGAVFVERVAVDTPAHVRQAKKAIKKAFQVQQAGLGFGIVEVLSTCPTNWG
                     LAPNDALQWLRDNMIPYYPLGNFKNVEVEEVK"
     gene            complement(48370..49449)
                     /locus_tag="PC414_000798"
     CDS             complement(48370..49449)
                     /locus_tag="PC414_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HDF2463207.1"
                     /translation="MAKILMKGNEAFGKAAIEAGCKYFFGYPITPQSELPEYLSRELP
                     KIGGAFVQAESEVSAINMVYGGAGAGARVMTSSSSPGVALKQEGITYAVGAEVPCVVL
                     NVMRGGPGLGSIQPSQADYFMSTRGGGNGDYRTPVFAPATVQEAVDMIMEAFDVADYY
                     RSPVMVVADGMIGQMMEPVEFRAPEKKRELPPKDWATVGTKGKRKPNVINSLYLEPEV
                     LEDHCWHLQEKFDAMEKNEVQYEMYKTEDAEFVFAAYGTTSRVVKSAIDILREEGIKA
                     GLIRPKVLWPFPFEAFNQIPNARNILTVEMSMGQMVEDVKMAVEGKLPVYFHGRPGGM
                     TPTPAEIVEKAKKIIAGELVAGGAR"
     gene            complement(49478..49693)
                     /locus_tag="PC414_000799"
     CDS             complement(49478..49693)
                     /locus_tag="PC414_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2463208.1"
                     /translation="MAKGKVSFNQERCKGCGLCVEACPVKIIQLDSNVINKKGYNPAT
                     VFEMEKCIGCASCATMCPDVVITVERD"
     gene            complement(49736..50413)
                     /locus_tag="PC414_000800"
     CDS             complement(49736..50413)
                     /locus_tag="PC414_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2463209.1"
                     /translation="MITNDKRIRIITGHYGSGKSEFAMNYVVKLRDMVSGKVAIADLD
                     VVNVYFRTREKKELMKSLGIQPIDSSINAPTLDLPAVSAEVMSPMVDHSYNSVIDLGG
                     DNVGARVIGRFSHLLKEGDYDMLFVINANREKTQTSEEVIQYIKEIEKSSKLKVTGLI
                     NNTHLIRFTTIDDVLRGQKVAKEVSEKCNIPIRYVACLENLVEQLPKDLEGEIFPIKL
                     YMREDWM"
     gene            complement(50403..51482)
                     /gene="buk"
                     /locus_tag="PC414_000801"
     CDS             complement(50403..51482)
                     /gene="buk"
                     /locus_tag="PC414_000801"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HDF2463210.1"
                     /translation="MSKIFKILTINPGSTSTKIAVFDNEDLVFEKTLRHSSEEIGKYE
                     KVSDQFEFRKQVIEEALKEGGVKTSELDAVVGRGGLLKPIKGGTYSVSAAMIEDLKVG
                     VLGEHASNLGGIIAKQIGEEVNVPSYIVDPVVVDELEDVARISGMPEISRASVVHALN
                     QKAIARRYAREINKKYEDINLIVAHMGGGVSVGAHKNGKIVDVANALDGEGPFSPERS
                     GGLPVGALVKMCFSGKYTQDEIKKKIKGNGGLVAYLNTNDAREVEERIEAGDEKAKLA
                     YEAMAYQISKEIGASAAVLKGDVKAILLTGGIAYSKMFTEMIADRVKFIADVKVYPGE
                     DEMIALAQGGLRVLTGEEEAQVYDN"
     gene            complement(51504..52406)
                     /gene="ptb"
                     /locus_tag="PC414_000802"
     CDS             complement(51504..52406)
                     /gene="ptb"
                     /locus_tag="PC414_000802"
                     /EC_number="2.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate butyryltransferase"
                     /protein_id="HDF2463211.1"
                     /translation="MRSFEEVIKFAKERGPKTISVACCQDKEVLMAVEMARKEKIANA
                     ILVGDIEKTKEIAKSIDMDIENYELIDIKDLAEASLKSVELVSQGKADMVMKGLVDTS
                     IILKAVLNKEVGLRTGNVLSHVAVFDVEGYDRLFFVTDAAMNLAPDTNTKKQIIENAC
                     TVAHSLDISEPKVAAICAKEKVNPKMKDTVEAKELEEMYERGEIKGCMVGGPFAIDNA
                     VSLEAAKHKGINHPVAGRADILLAPDIEGGNILYKALVFFSKSKNAGVIVGAKAPIIL
                     TSRADSEETKLNSIALGVLMAAKA"
     gene            complement(52710..53891)
                     /locus_tag="PC414_000803"
     CDS             complement(52710..53891)
                     /locus_tag="PC414_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463212.1"
                     /translation="MINRLKNNTKIKIISFLSAIALWMYVMAVVDPEETKGFEDIPVS
                     ISNMSDLKDKNLVIYPDEKLTADVYIKGSLSKVQSVKKEDIHVYGTIEDPIQGQKEVY
                     LKANIPQGVTLEFKHDVLVVNLEKNIKEKRDIKVEVSGNSKRNINKIELSKEFVDVSG
                     PRTLVKEVKSVRANLDVGNETDDFSKKLQLIPLNSDGTKVEGVTLSSSFVTAKIELLK
                     EKIVPIRVSLNDNKDNDVLKNYKLSKEQVTIKGKKEDIDKISYIKTKPIDLADLANGE
                     EKEVSLEIPEGITLEDSSITIKLNDTKQLSAEFLYNAGDIELRNIPTNMDNPNVISDV
                     DIKVVVESMEDLSKLNKKDISLYIDLSQGPEEDSKYKIKYETTYQFKKINIEPNIVEV
                     E"
     gene            complement(53888..54727)
                     /locus_tag="PC414_000804"
     CDS             complement(53888..54727)
                     /locus_tag="PC414_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HDF2463213.1"
                     /translation="MLDINSFLNGFFNIILRMSIYDLIDISIVAYIFYKIFMFIKDTR
                     AEQVFKGIIFLLLATQLSNTFKLHTVYWISLKALDYGVIAALIIFQPEFRAGLEHIGR
                     AKFNLFGKNVNTSEETLNRNIEEIVEALYSLSRQKIGALIIMERETRISDIINTGTII
                     DAEISRQLLINIFIPNTPLHDGAVVIRDSKVKAAACFLPLTESKDLSKDLGTRHRAGI
                     GVSEVSDCITLIVSEETGGVSIAKAGKLYRDISRERMMNILRSNLKTNTETRSFFKGG
                     IFK"
BASE COUNT        16668 a        10369 c         5832 g        22218 t
ORIGIN      
        1 gaataataaa tgtaatatta aaactcaata ataaatttat attcataagt ttaatttctt
       61 cattaatttt atcaactatg cctctactgc ctattaattt aatgcaataa ataataaatc
      121 ttttacaaaa agataataaa aatatagaaa aatatttttt attgtccttt tggttatggt
      181 aaaccacatt ctgcctgcaa tataactaaa tatatgggta gatagtaaaa ttaattttat
      241 tatttatata taattttata agttactttt caataatatt gactgtcatt aatttataat
      301 attcaataaa atattttaat ttagttaaga atctatattg taaaggagta ttaaattaat
      361 gaacttaaaa ttaatatgga taaatattct aaacccagta tttattaaaa atgaaataaa
      421 aagaaaaaaa atacaaatta ttatcttttt ctttataaac tttggtttaa cttattcatt
      481 aggtttactg ttatattata ataaatttat tgatccagaa aactttgcat cattcatgat
      541 gatattacct ctatcttcag ttgccattgc aaagttttat actgaaggat taaataatga
      601 tatgtatgaa ttttattcac taatcatatt attttttctt atttattcat tattaattgt
      661 tcttaagtta ttaaatctaa taaataataa tgaatttata attataaacc taatattaac
      721 ttttataagt agcttatcaa ttataatata ttcatcaaat aaaaaaaatt tatatcctgt
      781 aaaaaatatt aaactaggtt ctttattaat tatttacttt atgatttctg taattgttcc
      841 aacatcattt atactaatac tacaaggggc tcaacctaat tatagtggta tcataaaatt
      901 tatatttact ccaataacat cattaactac tatttatatt ttttttagtg aagaatatgc
      961 ctggagaggt tttttacaga atattttttt tgataaattt ggtaaaaagt taggagttat
     1021 aatattaggt atgtgttgga gcttgtggca cttaccacta atatttacat tatatacacc
     1081 tgaagcgcca atattaggaa taatattacg ttcaatacat atagttggaa tttcaatatt
     1141 tttaggatat ctttatataa aaactaagaa tatctggttt tgtgctataa ttcatgtttt
     1201 aaataatagt gcttttctta tcacaaattc taaagaatca actatcactt accatgatat
     1261 actaatagta tctatggttg ttttaatttt ttatgttcca tttctattta caaaagaata
     1321 taaaaataac ttggagtaaa tagaatatcc aagttatttt tatatttttg caaatttcca
     1381 acaatcctac agaatacttt aagcttagaa gatattatta aaatttatta taatcaacta
     1441 attcttgata ttggtcacat ttactttatc actaacaaaa tcatacttag aatctctata
     1501 gtaataccat acaaatataa agctaagtag attaacaaaa gtagtcaaaa ttccaccttc
     1561 aataccaaac attcctccat ttaatagatt accatgagtg atttgtgtag ttataatccc
     1621 tggaacttga agtccactta caggcatacc atatataatc ccctgaaata cattccatgc
     1681 aaaatgataa cctatacaca tccatatatt cgacgatttt ataaacatat atgaaaaaag
     1741 aatcccaata attataatat taattacaga caaaaaagta acatcaggat tccatatatg
     1801 aatcaaccca aaaataacag atggaacaat cattataaaa taaatatttt tacatcttcg
     1861 taaaactgcc ataaaaaaac cacgatttaa aatttcttct gcaaaagcaa ctaaaataaa
     1921 gaccagtatc caagaaactg ttaaaggtga aatttttggt acccatgaag taacatgacc
     1981 accaccaatt gttattgtta taataaagat tactgtacaa ttaaatattc ctaataacat
     2041 tcctacatat ccttcttttt tcttaattct ataagaatgt aatcccattt cactaatagg
     2101 atttttcata ataaatctcc atacaattat aggaataata atcataagtg aatctgttag
     2161 aatttgaaat acaatctgta acccaacatc atttaaccaa ttagcatatt ctgaaaaata
     2221 gtttgttttt aaattaatgt cacctgtagc aaccaggatt tttcttatta tttccacaag
     2281 tatcatgctt aaataatact gaagtatgta aaaggtagcc ataacagcta taataatcca
     2341 ccctgaacgt acttttttgt ctttattctt aataatcaag ttgctccatg atttgttcat
     2401 atagatgtct tcctcctaac tattattttt agttgtttct aaaatatatt ctattaaatt
     2461 ttttcatagt agtattttac cacaaaactt ctgcttcaat gaagtaagcg taatatattc
     2521 tctaactgta atttaaacta taaaaaatat ataaagtata tttaaagcaa tatttatgga
     2581 cataaagcaa tataaacaat atataaaaca tataaaactt tacttaatta tttctacata
     2641 aaaaccacat aaaaaaggat gtaactctag cttttagtgt aacatccttt ttttaattaa
     2701 aacttgtatt taattttaga aagctgtctc atgaccaaca taacaagcta taccattatc
     2761 caaaaatata tcttgtaatt cttcaagatg accttcatca acatcaccct tatcagaata
     2821 ctcatattcc ttgcctaatt gaatccattt tgattctcct aatctgtgaa atggtaatag
     2881 attaatttct accaaattat ttttgtgcat aaaagatata atatcactta tattttcaac
     2941 actatcatta aacccagaga taactggtac tctaagtact aatcttccat tccaatcaga
     3001 atttgcaagg tttgatatat tttctaatat taaatcatta tacacacctg tttgttcttt
     3061 gtgtttttct ctatccatat gctttatatc aataaatgca aaatcaatat cgttaaatat
     3121 tttattaaaa acttcatttg atacacatgc actcgtttca atggcagtgt gtatatttac
     3181 ctcatgacac tttaataata cttcatgtaa aaattcatgc tgaagtagtg gctcacctcc
     3241 actaaatgtt actcctccat tacttctcca attgttagaa tcacgtttaa ttacctgcac
     3301 aagttcatct acagtgtaag gttttgcaca taatttaaac gcattataat aacatgagtt
     3361 gacgcattca aaactttcac agtccttaca gatgttccaa tctataactg gcttattatc
     3421 aagattaaag ctcaatgcac catttttaca cttaccatga catacagtac atccattttc
     3481 atattgacaa gataattcac taaacatcat atgtggtctt acagtccaac tttctggatt
     3541 tgcacaccat ttacaactca gtggacatcc atttaaaaat acagttgttc tacaacctgg
     3601 accatcatga actgaaaagc tttgtacatc aaaaatcatg ccttctaatt gcttttgact
     3661 actcattaaa cacatgtcct tcacaagtaa ttgcattcaa tgttgaatat acccagtctt
     3721 ctttctctag gccaacacat ttgcctatac cttcatcatt agcttcaaca aaatttttac
     3781 aatttttaca ttttggcatt acttttatct ctggacatgc tgcatcatct aaattaatca
     3841 tttgttttga taatctacaa attcctttgg ttgcatctac tgcacaaaaa ttcatacaat
     3901 cactatgctt tctcatattt tacacccctt catactctgt tctagcaatt acttcatctt
     3961 gtattggttt acctaattca caccaatatt gagtaaaccc agcaacacgt accattaatt
     4021 gacgataatt gtcaggattt ttttgtgcat cttttaatgt ttctgaatct acaacattat
     4081 attgaatatg gaacccacct tttcttaaat aacttcttgt taaatctaat aattttttag
     4141 ttccttgttg tccttttata gtagttggat gaagttttaa attcatctga gaattttgaa
     4201 ctactgcttg gtcccaaata gttgcagatt caaataatgc atatactcca tttttatctg
     4261 ttcctgcata tgcagagact gaaccatctg aatatgttgt tccacttagt ctaccatctg
     4321 gagtagccaa ggtagcagct ccctgtggcc catgtgtaga aacagatatt tggcaagggt
     4381 acatcttctt agcatataaa gattgtgctt cttcacatac ttttgataac catacctcat
     4441 agtttttaag aatattatct gcatacaaat cattatttcc atatttaggt gcatcaagac
     4501 aggccttata aatagcatca tatttaccag tcttatctac ttttacttgg tctgccatag
     4561 aataatttcc tacttctaat gcatctttaa atccaaaatt atttaatatg gcatccttca
     4621 tttcctctat tgtaaatgct ttgtcatcat atacaagttt cttaagtgct gcaaatgaat
     4681 tgaccatagt aacagtacca caagtttcta cgtttaatgt tgcattataa cgatatccca
     4741 tatttcctat atgttgacct ttgtctaaac agtctggctt tagtagtgaa tttattatag
     4801 aagtatcaaa ttttctccat atatctaact caatgttatt tgctctttct aaaacattta
     4861 ttgcttgttt ataataatct ttaaatactt ctatgacttc ttcataagta tctaatggct
     4921 tattatgagg ctcaaatact tgtatattca tacgatagtc tttaccattc ataagaacaa
     4981 gttctaaaat ttttggatta gctataaagt gaactccaga gccagcagat tgacctgctc
     5041 cacctgcaat agagtatgtt tttccgttta aagttaactg tttccaacac cctggagacg
     5101 tttccaaaca tccacctaat gcaaatgctc ttgcttcttc aacagtcata ccttcatcag
     5161 caaattgttt catcataaat gttgttccat ttgagttgtt tacccatgct ggataaccag
     5221 aaccaagctt tgtacattca gcagctttca ttaagaaatc ttctggaagc ttttcatcat
     5281 atagcatagt taaactaggt tgaggagttc tacatctcat acttgcttct aataataact
     5341 cttctagttc attagctcct gtagaaccat cttcttttaa tccacctata gataaagtat
     5401 taaatgtatt gcctgatagt actcctccat ttacgcccgc tgaagcaaaa cagtcaatac
     5461 aagtgatttt tattctataa agctctaata attcgataac ctcttctttt gtaattagtc
     5521 ctttttctat atcttgttca taccaaggat ataaaatttg accaaaacgt cctatagaca
     5581 taccagatat agcatcctca tttactgatg ctatatgaat tgtataagtc aactgaagag
     5641 cttccctaaa tgtacgaggt tgtttatgtg caatatgttc taatatctcc actaaatctg
     5701 agtattcttt tttagcttct aaatctgtct ctatactttc taaatatttt gcatgttttg
     5761 cataatttaa aatccaagtt tgtaatcctt caataacttg aataacagcc tcataaaaat
     5821 atagacggtc cataccaata agcccatctc cagatgcatt tcctgcaact gcctttttac
     5881 attcttttgc catttctata attccatcta atccataatt taaaggataa aaataattta
     5941 taacttctct accttgagga agtgtatacc ctgaatcaaa catgcatata agtgtactca
     6001 ttaggttttc ttttaaatca taatctggca tcattttttc atagtgaaaa cctaaatctt
     6061 caacagactt tccaacccat tcctttgcca tttttacaag tacaggtact tcttcttttc
     6121 tcattccgaa tttaccagct attgaaacga catttccaaa actttcagta acatttcctc
     6181 caccagaccc aaattttgta agttcatcag ctgtgttact ggctaatttc tttgcctctt
     6241 ctctcatttg ttctccttga gcaacaaaga agctttcact tacccatggc attggaaaag
     6301 aacctctata atggcgtgtc ttttgcatta ctattttctc accaggtatt atatttggtg
     6361 tcatatgtga aaaagcagct ttaagagctt tagctcttct aactactggt atatcaccat
     6421 ctgattttct atattctcta ttataccaat atggaaattc catatcagca gtagataatg
     6481 tattataata tacatctaat aagtcttctg ctcttttact tgcttttttt tcattaaatt
     6541 cacttaaatt ttctttattt tgaaaatttg atttaatatt ttttgcttcc aaaatacttc
     6601 ttattttgtc ttctttactt tgactcattt cttcccctcc ttaatctttc tataattagt
     6661 ataaaacttt ttaagaatga aaaaaaaggg tatattaccc actttttaaa ataatctttc
     6721 tatagaaaaa aataaacttg aagcaaatct aagccaaaga caatctgtat atcctaaaat
     6781 ttgttgcaat tcattggaat ataaagatta taaatagttt tacttcaagt tttaatatat
     6841 actatattta tttttattat atttaatttc acttctactt acttttttga aacaatactt
     6901 ttatatttac aaattcatct aactctactg atttatcttt aaatttggaa ctgcatttaa
     6961 actcatatca tgcgttttcc catttataaa attataatat cctgcacacc ctatcatagc
     7021 tgcattatct gtgcataaaa ttagaggagg atacttaaca gtaataccat tgtctttagc
     7081 aagctctgtt attttagctc taagccccga attagaagca accccaccag aaagagttat
     7141 gatattgtat cctttgtctt ttactgcttt tagtgcttta gtagataaaa cctctactac
     7201 agcctcttgg aaagatgctg ctacatcttc aactactatt tcttcatttt tcattctttt
     7261 tccatttaga taatttaata cagaagattt taatccacta aaactaaaat catagctatc
     7321 ttcttctaaa taggctcttg gaaattctat agcatgttta tttccattct tggccagatt
     7381 atcaataata ggacctcctg gatagcctaa attcatagct cttgaaatct tatcaaatgc
     7441 ttctccagat gcatcatctc ttgtttttcc taaaatttca tacttgccat aatcttttac
     7501 ttcaaccaag tgagtatgac ctccagatac tattaatgtt ataaaaggag gttttaaatc
     7561 cttatgctct atatagtttg cacttagatg cccttctata tgatttactc ctacaagtgg
     7621 aatattcaaa gtatatgcaa gtgctttagc ataagataga ccaaccaata aagcacctac
     7681 tagcccaggt ccatatgtaa ctgcgatatg gtctatatca ttaaaaccta tatttgcttt
     7741 gtctaaagct tcttgaacaa ctatatcaat attttccaca tgttttcttg aggccacttc
     7801 tggaactaca ccaccaaatt ttttatgtgt ctcgatttga gtagagatta tgtttgaaag
     7861 tacttctcta ccatttttta aaactgatgc agctgtttcg tcacaactac tctctatagc
     7921 taatgttatt atatcactca taaactctac acctctttta attggttcca cattataatt
     7981 gcatcttctt tattatcact gtagtattct tttcttattc cacccatttt aaatccatat
     8041 tttctatata aattttgtgc tattttattt gatgctctaa cttctaatgt catagatgca
     8101 atattattat ctttacataa atcaactatg ccttttatca acttgtcccc tatttttttc
     8161 cctctatagt cactatgaac agccacatta tttatatgcc cttcatcaag tataagccaa
     8221 attcccacat atccgacaat ttttccattt aacttagcta ctatatacct tgctacttca
     8281 ttagacaact cttttctaaa agactctttt gaccaataat cttcaaaaca atttttttct
     8341 acttcaaaaa cttcatctat atcttctgta gtcatttctt ctatttttat atcatctact
     8401 attagttttg tattatcttc cattatttag cctcttcatt ttttcatcat attgtacttc
     8461 agcttgagat tttctgatat acataggatt tatcgtataa caagtatgta catctatatt
     8521 tttattatat ttatttaatg ctaaagagca taagcttcct gcgttagata cattattagc
     8581 tggtgaaggt atttttatat tttttatatt ctctaacttg tctttatatt tgtatacagc
     8641 ttctccaact attataaatt cttcattagt tgatacaatt tcatctacta aagcatcaaa
     8701 ctctacaaca tcaacaccat ctaattctat catctctcca ttttcaaact tgtatttagc
     8761 tgaataaact tgatttttct gtgcatctaa tatacaacat atctttctat cacagaaatt
     8821 tatgttattt gctagagatt ccaaagaatt tactgctatt attggtatat tatttacatg
     8881 ggccatagct tttactgtag ccatacctat tctaagtcct gtaaacgaac ctggacctat
     8941 acatacagct aataaatcca tatcttttat acttaaatca ctcatactca acatattttc
     9001 tatcattggc ataagctttt gtgaatgagt ttttttgttg tttactgtaa actcacatat
     9061 taagttatca tcttcaacaa ctgctacact tgctgccata gaagacgtat ccatacctag
     9121 tattttcatt atttaatcaa ctcctcaact atctctttat aaaactctcc ttttggagtt
     9181 aatatcattt ctcttccttc atctttatat ctcaattcta tattcaaata ttcttttgga
     9241 agtatatctt ctattagatt agcccattct attatacaga ttccatttga atttacatat
     9301 tcatcataac caatatcata catttcatca ctactaccta ttctataaac atcaaaatga
     9361 tacaaaggta tttttccttc atattcattt attatagtaa aagtaggact tgttatataa
     9421 tcttctatcc ctaatgaatc tgccaaactt tgtgtcattg tagttttacc agctcctaaa
     9481 tctcctacta aacagataac actgccttct tttaaaagtt tacctaattt atatcctatt
     9541 tctctagttt tattttcatt ttctaaatat atttttgcca tcaattcatc tccattttga
     9601 tataatcgtc atgtgaattt atatcactgc ttaattaatt atagtataag cctagattat
     9661 tataaagtac ttgtctttat ctttttttat ctttcactat tgatggctgt atctacacct
     9721 tatatacaat catcaatagc aatttttaca tactcttttg ctttatggtt ttgcttcaat
     9781 gcttctttac atattctctc agattgctct tttacatact taagagactt atagttctct
     9841 tttactgctt caatacacat tttttctgtt tgatttttta tatacataag catttttcca
     9901 ttttgtctta caatctctat acatatatct tctgtatact tatctatgta acttagtaca
     9961 tctttaatat tatatttttt atttaaatat cttatagcat ttatacatat ttcttctgtc
    10021 ttattgttga caaattgtat acttcttcca tcttgtttga cagcttctat acatatttta
    10081 tcagtttgtt tatttacata ttgtaatgcc ataccatttt gtcttacgga ttctatacac
    10141 attttttccg tttgattttt tacatatttt aatgctttat aattttgctt aatggcctct
    10201 atacataatt cttcactctg ttctctaacg tatgcaagac acctgccatc ttttttaatt
    10261 gcttctaaac atatccttct tgtttgagtc tctaaagcct ttagtatact tacaacatct
    10321 atattggaag aatgacttat tgcttttata ataagccctt ctgttttatt tcgtacatac
    10381 ttaagtgcat tacaatcttt ttttatagct tcaagacaca agtattctgt ttgttctttt
    10441 acatattcaa gtgctttata attatacttt attgcttcta aacataattc ttcggtttga
    10501 ttttttacat actctaatgc cttgtaattc tgccttattg ctaaaataca cataccatca
    10561 gtttgatttt ctatattttt taattcacga taatcgtatt ttaccctttc catattttca
    10621 atatattcat tagtgatata ctttaaagaa tctcctaatt ttgtatcata aataccaata
    10681 tgaatatctt tccaattact attaaaataa taccctttat catctaaaaa taattttttt
    10741 atttttttag ataaatgcat gaatatacac ccctatcatt attaaattag ttattattaa
    10801 aagtttgatt attaatcttt atagtttttt aataataact ttattttttg tttattataa
    10861 attcgctctg tatattgtag ataccttttt catattttaa tagttaatcg ttttaattct
    10921 tcctttattt taaaatttaa atttgtctaa atatttctta ttttatatac attttttgat
    10981 aaattaataa agccataata tatttaatat atcaatcaca atataggact gaagttataa
    11041 ataaattatg gctattcaat acatttaatt ttatattttc ttgtctaatt atttctttat
    11101 tatatttccc atttttttat aaagtggcaa tgttactaaa gatattataa cagccttcag
    11161 cacattaaat ggagctatca tccaaattac aaaagtgaat aaatcatgaa cttgaggatt
    11221 tatagctgaa cctaaaccta ttatagcatc tagtcccata agttgaccat aaagtggcaa
    11281 cattacaaaa taatttacta atgaaccaat tatagtcata gatattgttc ctaatacaaa
    11341 accaatcagt acacctttta aatctccact tctcttatat gaataacaaa gtatcattac
    11401 ataagaacca ccaattaata tatttgcaaa ttctcctatt cctccagttg ttgttgctgt
    11461 tattgcttgt aacacatttt ttactacaac tattccaaat ccaactaatg gtcctagaga
    11521 cattcctccg aatattgctg gaatatctga taagtcaatt tttaagaaat ctggaaatat
    11581 acctggtatt ggtgcttgaa taaacattaa taaataagat attgcagata aaattgacat
    11641 tactactaaa gttcttgttg aaatcatatt gcctcttttt gctgtgtttt gcataattat
    11701 tttcccccta atcaatatgt ttatattttt taggaaaaac aaaaaactcg aagaccctat
    11761 cttcgagttt ttatggatat agtttaaaca aactaaaata aacttattta tcttccataa
    11821 atatcttctc tcatccagac tttactgtcg gctttggaat ctcaccaaat caaccaccta
    11881 agtagttcgc gggctatacc gccggtaggg aatttcaccc tgccccgaag atcttctttg
    11941 attttttaat tttccttaca ctcttatatt agttaatttt ttaataattg tcaagttatt
    12001 ttttctcata cactactttg ccatttatta tagtgtaaag tacattactt tgaatctcta
    12061 gtggagaatt atcccaaatt actaggtctg catctttacc aacttctata gagcctactc
    12121 tatcttctat tcctaaagcc ttagctggat ttatcgtaat tgattctatt gctttttctt
    12181 ctttcatacc atgttttact gcaatacctg cacatattgg aagatattga actggtatta
    12241 ctggatggtc tgtcattata catacatcaa gacctgcatt tgaaagtatt cctgctgtat
    12301 taaatgtcaa atttctaagc tcaaatttag acctttctga caaagatggc cctactatta
    12361 caggaaaccc ttcttccact aattcatcta caataagatg accttctgta caatggtcta
    12421 atgtaagctt taaattaaat tcttttgcaa ttcttattgc tgtaaacata tcatctgctc
    12481 tatgagcatg tgccttaaaa ggaatttctc ttcttaaaac tggtattaaa ctttccattt
    12541 ttatatcata ttcaggtttt tcacagtctt catcatcatg actctcatac atatcaatat
    12601 cctctaaata ttcttctgct ttttttaggt tttctctaag taaagcagct atagccattc
    12661 tagtttgagg agatttatca tcctgaccat aacagctctt tggattttct ccaaaagcaa
    12721 ttttagatgc aactggattt tttattacca ttttatcaat tctttttcca catgtcttta
    12781 tagctataca ttgaccaccc ataacatttg cacttccagg ggttgtacaa acagaagtta
    12841 ttccaccttc aaaagcttct ttaaaagttc tatccattgg attaattcca tctataggat
    12901 ttaattgtgg tgttattggg tcagtctctt catttccatc tgctccttca aaccctatac
    12961 catcttccca taaacctaag tgagtatgag catctataaa tccaggaaac acaagtttat
    13021 tactagcatc tattacctct acatctaaag gagctatcaa atcttctcca atttctatta
    13081 tttttccttc atctataagt atatctccat gaattatccc atttgttatg gtatttattt
    13141 taccattttt tataaaaatc attcattttc ctcctgattt tcaagactat cgttcttatt
    13201 ttaatatatt ttcttactat atacttttac aaatttttaa catttataca ctcgagtaat
    13261 cttatttaca aatatagaat ctaagtacta taaccataca taaacttatc tattttttca
    13321 ttgataaggc aactcttttt ttgtttaagt caattccaat gacttttaca tctactatat
    13381 cacctactgt aacaatactc atagggtctt tcacaaaact attactcatt tctgatttat
    13441 gaactaatcc gtcatttttt attccaatat ctacaaatgc accaaagtct acaacatttc
    13501 ttattgtacc ttttaaagtc ataccctctt gtatatcttc aatttttaag acatcagttc
    13561 taagtatagg ttttatacct tcttctcttg ggtctcttcc tggttttttg atttcagcta
    13621 tgatatcttt taacgtaact tgtcctactt ccagtttttc acttaattcc ctcaagccaa
    13681 tttcttttat tttttcatct atttcaccta tattcttatt ctttacatca tctaaagaat
    13741 atccaattat ctcaatcatt tttttacata tatcataact ttcaggatgc acacctgtat
    13801 tatctagtgg atttttaccc tctaagattc tcataaatcc agcacattga gtaaatgctt
    13861 gaggtcctag tctttttact tttttcaatt gtgctcttga cgtaaaatct ccattttctt
    13921 ctctatatgc tattatattt tttgctatag ctttacttat tcctgcaaca tgttctagta
    13981 gggagtatga tgcagtattt aaatctactc caacactatt tacagaatct tcaacaactc
    14041 catctagtac ttcttctaat ctctttttgt ttaaatcatg ctgatattga ccaacaccaa
    14101 tacttttagg gtctattttt acaagctctg ctaatgggtc ttgtaatctt ctagctatag
    14161 atatagctcc tcttattgat acatttatat ctggatgttc ctcatttgca agttcagatg
    14221 ctgaataaac agatgctcct gcttcactaa ctatcacata ctgcacttcg ctatctattt
    14281 cttttatcat ttctgataca aaagtttctg attctctaga tgcagtacca tttccaattg
    14341 aaattatatc tatatcgtat ttttcaatca atccttttaa aactttcttt gctccctcta
    14401 catcattttg agggtctgtt ggatatactg ttgtataatc tagtagttta ccatttttat
    14461 ctactacagc tattttacat ccagttctaa atgcagggtc aaatcccata actactttgt
    14521 ctttaactgg tggttgaagc aataaacttt taacattctt tccaaataca cttatagctc
    14581 tttcttgagc tatttctgtt aaatgatttc ttatttctct ttctattgat gggaaaatta
    14641 atcttttata agaatcttct attgaactta cgatttcttc tttatttttg aaatttttat
    14701 catttacata ttcgtttatt atgtaattta aaactttatc attatttatt tccaacttaa
    14761 cctttaggaa gctttctttt tctcctctgt ttattgccaa aactctatgt ggagccatac
    14821 tttttactgc ttcgctataa tcataataca tatcataaac cgatttctca tcagtagcac
    14881 ttttgctaac tatcatacct tctcttaaag ctaattctct tatatacttt ctaatcttag
    14941 catcatctga gactagttct gcaattatat ctctagcacc ttttagagca tcttcaatag
    15001 aaagaacttc tttctcttca tcaagataat tctttgcctc tatttctata ttatctaaat
    15061 tattctctag tattgacaat gctaaatttt ctaatccttt ttcttttgct attgttgctc
    15121 tagttctttt cttttgttta tatggagcat atatatcttc tacttcttgt aatgtttttg
    15181 ctttttctat attttgtgtt atttcatctg ttaatttccc ttgttcatct attagtctaa
    15241 ctacatcatc ttttctactt tgaagatttc tcaaatacat taatttttca taaaattctc
    15301 ttagtgtaac atcactcatt tcgccagtca tttcttttct atatctagct ataaatggta
    15361 ttgtatttcc ttcatcaata agttttaatg tattatttat ttgttcatct ctaagattaa
    15421 actctttttt taagatttga tttatatcca atttattcac tcctaatgtt agtttttcat
    15481 aagtataaca aaacagaacg aaggctgagg gttatcgtcc tcagcctaag tgctgtaagt
    15541 gcatattaag cactaacctt cttatacata tatcttaatt acagtgcata tttattaaac
    15601 tattttattc atttttaagt attcattaat aaacaagtct atatttccat tcattacact
    15661 atctacattt ccaaactcag catttgttct atggtcttta accaatttat atggttgaaa
    15721 aacatatgac ctaatttgac taccccaagt tatttgtgag tatttgcctt gtatatcttc
    15781 tattttttcc ttttgctcta actcttttaa ttctatcaat ttcgccatta aaagtctcat
    15841 agctctgtcc ttatttagat gctgagacct ttcattttgg cactgaacta ctacaccagt
    15901 agggatatgt gttattctaa ctgctgaatc agtcgtattt acatgttgcc ctccagctcc
    15961 agaagctcta tatgtgtcta tctttaaatc tgatggatta atttctactt ctatattttc
    16021 atctaactct ggtgttacat ctatagatgc aaatgaagta tgtctttttc ctgatgggtc
    16081 aaatggagat attctcacta atctatgaac tcctttttca ctctttaaat acccataagc
    16141 atttgttcct tctacaagaa ttgtagctgt ctttatccct gcttcagggt ctgatattat
    16201 atctaaaagc ttaactttat aacccttact ttctgaccat cttatataca ttctcaaaag
    16261 catttgagcc caatcttgag catctaagcc accagtacca gcatttatag ataatatagc
    16321 attattttta tcatactctc catttagaag agtctttatt ttcatttcag ataatttttc
    16381 ttccattgat tctatggact tttctatatc tctttctaca gaatcatcat cttcttctaa
    16441 acctatttca attaatactt ctatatcttc taataagctt tttaagcttt catattcttc
    16501 taatgtctct tttaaagatt tattttcttg aagtactctt tgagcgactt cattatcatt
    16561 ccaaaaatct tgttgattca tcttttcttc attaatttgt atatttttca tcaatgaagc
    16621 tatgtcaaag agaaaccctc aattcttcta aatttgaagc catctgctcc atactatgtc
    16681 tatactcttg taactttaac atttaatcct ccgagattat cttccacaac agtttttata
    16741 ttttttacca cttccacaag ggcataagtc atttcttcca accttttcat cttttttaac
    16801 agttttactt gttggaagtg ttccatctga tggagaagat agattttcaa catctacaac
    16861 tgcttttctt tccactggag tttctattgt tatattaaat aaatatctta cagtatcttc
    16921 tttgatatgt ttattcattt catcaaacat atcaaaacct tccattttat aagcaataac
    16981 tgggtcttgc tgacctacag cacgaagacc aataccttgg cgtaattggt ccatagcatc
    17041 tatatggtct atccaatggt tatcaacagc ttgaagtaag attactctct caacttctct
    17101 cattctctca tagccaactt gttcttcttt tgaagtatat atcttcatag ctatttcata
    17161 aactttttct gttatttcaa ctggattaag tttttctatt tctggtattt ctatgctacc
    17221 ttttggcaag aacagattat acatatgttc tttgaatcct tcttcatcaa atcctttatc
    17281 ttgtgtataa agagtaactg cttcttctat tatagaatga gtcattgatt gtatttgttc
    17341 ttgtaaatct tcaccttcta atacacgttt tctctctgca tatattattt ctctttgttt
    17401 attcataaca tcatcatatt gaagtacatg ctttcttatt ccaaagtttt taccttcgac
    17461 tttcttttgt gctccttcaa tagacttaga taatattcta tgttctatcg gcatgtcttc
    17521 ttcaagacct attttatcta caataccaga tattctatca ctaccaaaca atctcataag
    17581 gtcatcatca agacctatat agaatcttga actccctggg tcaccttgac gtccagcacg
    17641 acctctcaac tggttatcta ttcttcgaga ttcgtgtctt tctgtaccta ttatagcaag
    17701 tccacctgct tctacaactt gtttttgttc ttcttgtgtt tgttgcttaa acttttcaaa
    17761 tagtttttca tactcttctc tagcttcaaa tagaatttca ttacctttta caggtatacc
    17821 ttctattgga gtatctactc tatttactat ttcttcttta aatccatttc ttctcatttc
    17881 tctcttagtt aaaaagtctg gatttccacc tagaactata tctgtaccac gaccagccat
    17941 attagtagct attgtaacag ctccaagtct accagcttgt gctattattt ctgcctcttt
    18001 atcatgatgc ttagcattta atacctcgtg ctttatgccc tttttcttta gtatttgtga
    18061 taataattct gatttttcta ttgatactgt acctacaagt attggttgat tgactttgtg
    18121 cctttcaatt atttcttgtg ctacagcatt aaatttacct atttcacttt tgtatacaca
    18181 gtctggtaag tcttccctta tcataagctt attagtaggt acttggaaaa catccatttt
    18241 gtagattgct ttaaactctt cttcttctgt ttttgctgta ccagtcatcc cagaaagttt
    18301 tttgtacatt ctaaagtaat tttggaatgt tacagtagct agtgttttag attctctttg
    18361 tattttaaga ccttctttag cctctattgc ttgatgtaaa ccttctgaat atcttctacc
    18421 aaacataagt ctacctgtga actcatctac tattactatt tctccatctt tagcaacata
    18481 atcaacatct ttcttcatta ttacatgtgc tcttaaagct tgatttatat gatggtataa
    18541 ttctgtatga gatatatctg ttatattatc aacattaaaa tacacttctg ctttttgtat
    18601 acccgatgct gttagagata cagccttatc tttttcttct aattcataat catctggttt
    18661 taaggttaaa acaaatgtgt ttgcatctga atataagtga gttgatttgt ctccaggtcc
    18721 agatatgata agtggtgttc tagcctcatc tattagtata gagtcaacct catccactat
    18781 agcataattt aatcctcttt gaactctttg ctctttgtgt attaccatat tgtcctttag
    18841 atagtcaaat ccatattcat tatttgtacc ataagttata tcacaatcat attgttcttt
    18901 tctaactttt ggattttgac cgtgtataat aacgcctaca ctcattccta aaaactcata
    18961 gatttttgcc atttggtctc tatcacgttt tgcaaggtaa tcatttactg ttacaacatg
    19021 tacacctttt ccagtaagcg cattcaaata aacaggagca gttgcaacca aagttttacc
    19081 ttcacctgtt ttcatttctg ctattcttcc ttgatgtagt actatacctc caatcatttg
    19141 aactctgtag tgtctaagtc ctaacactct cttagaaact tctctaacta ctgcaaaagc
    19201 ttctggaagt atatcatcta tactttcacc atttgctaat ctttctttaa atatatttgt
    19261 catattttta agttccgagt ctgccataga ttcaaattta ggttctaatg aatctattat
    19321 atcaacagtt ttattgaact tttttaattc ttttttatct gccatgttga ataaattatc
    19381 cataaagctc atattttaca tctctcctcg ttctttattg tactattatt cattttacca
    19441 taaaaactat acttttaata ttaatttaaa aagctatcga taatttattt atcgatagct
    19501 aatatgatta aatctaaatt gctttattca tgttctatta taccatatcc accattatta
    19561 cgcttataaa ctactgctat ttcatctgaa tctatgttct tgaacatata aaaatcatgt
    19621 cctattaatt ccatctgtaa tacagcttct tcttcactca ttggcttaac actaaatttc
    19681 ttacgtcttt gaattactaa ttcttgattt tcatccccat aattctcttg ttcatcagct
    19741 agatttatct ctgcatcttg gaatcttata ctcttgttgt ctttatgttt atcttgtaat
    19801 ctctttttat atttttttaa ttgtttactt aatttatcat atacaacatc tatagctgtg
    19861 tataaatttt cctgcatatc ttctgctcta attataggac catttacagt agctattgtt
    19921 acttctattt tttgtcttgc ttttctcgca cttactgtaa cttttacatc agtgtctgga
    19981 tgaatgtaat tatctaactt acttagttta gtttcgattt tattttttat tgcatcagtt
    20041 aattcgattt gttttccaga tataattatg ttcataaata catctccctt caattatgcc
    20101 agtataagaa aatttatact ttaataatat attctacacg aactctcata atcctctttt
    20161 tattatttac ttttatgatt ttttctattc aattgataga attatttatc atttttagtt
    20221 acattgatac ttatgtggat taatattatt acaaacacct gtggataatg tggataaaac
    20281 tgttaataac tcttgttttt gtttatttaa agctattttt actcctattt ctatccacaa
    20341 aaatctccat taattgttta ttaatatgtg aattcatgtc ataagatggt aattttagga
    20401 aattatgtgt attatatgcc aatatacctt tataattttt taataaatat aaaccctcta
    20461 aaacctccat aatctatctt ttctttcatt atttatattt aaaactaatt aaaagttata
    20521 aagcatactc aacaataatt tcatatttaa tacctattaa cattcatata cccaaattat
    20581 aattaactta ctctctttat ttaaactaag tattttatat tcatatacta tcttgaaatt
    20641 atatttacga gttgtttaag cttttgtcta tctatatctt caaaaggact gttatttatt
    20701 ctaatcgctg taccaaaatg aaaattactt gccttagtta attcctttat cttttctata
    20761 ttattaaaat taagacctcc acctaaaaga atttttatgt gattagaact aagcatcata
    20821 ttctttatca tttgtatgtt atcaactata cttcctttcc ctcctgatgt taatatattc
    20881 gtaattttat cataatcctt caaaatttta acactatcta ttgtatttga ttcatctatt
    20941 gctctatgaa atgtaacatc aagattatca caacatttca ataaaacatt tagatttttc
    21001 tcatctatac tattgttttt atccaataca ccaaatacaa ctccattagc accaatctct
    21061 ttaaccacac ttatatcttt ttgcattata cttatatctt ctttactgta tacaaaactt
    21121 ttcgcatgat gtcttatcat tacgttcaca ggtatcttta cactgttaac aacactttct
    21181 ataagaccaa aacttggtgt caatccacct tcagttaaag cactaactaa ctctattcta
    21241 tctgcaccac aatcttctat aattttagcg tcttctactg acattcctat tatttctaac
    21301 ataactactc ctttgctaat tcatatattg catttgcaaa tatctttgca tttaatatta
    21361 tatcttctat atccatgtat tcatcaggat tatgagctat tcctttttgt cctggaaatg
    21421 aaggtccaaa cggaactata tttggcatta cttttgcata cgtacctcca ttagttgtta
    21481 cgggagttcc atctaatgat gtaacccttt cataagctag ctgaagcttt ttaacaagat
    21541 tactctcttt atcaaaatat acaggattaa aattagaaat tacctctata tcgatactat
    21601 ttggaaaact agcttttatt tcatccaata catctttcaa tttataacta aaatcgtatt
    21661 ttacctttat atctaatata gtatcactat tttctatatt cagctcttta tatgtaacaa
    21721 gcacattttc agacatattt tctataaaac ctaggtatac atcttcttca taattgattt
    21781 tactttttat tctaatcttt gccatgcctt tttcaccata tacaacagga tatttacaat
    21841 ctggtgtaaa tcccataata ggtgcttttt ctttttctaa ataatatggt atatcttcaa
    21901 atccactttc ttcatttgta ccaaatatta ttctaactct cttatctaat tttaagttta
    21961 attctttaat agcatataat ccatataagg cagacattat aggtccttta ttgtctagta
    22021 ctcctcgtcc ataaattctt ccattagttt cttctccctt ataaggctga tgcttccaac
    22081 catctccttc atgtacaaca tctacatgtc caattacaca aacataatca tcactatctc
    22141 catgctctgc ataaccaata taattatcca agtttctaac cttaaatccc aatttttctg
    22201 atatttctaa tgctttatta agtgcctccc ctactttttc gccaaaaggg aagccatttt
    22261 ctgactcccc tttgacacta ggtattttta caatatctat aatatcactt aataaatctt
    22321 cttttaactc atctatttta ttacttatga tattcataaa aatctcctta ttataaaact
    22381 ctattttaat tatgtaacca ctactttact cacttacttc tattggtgct tttattctag
    22441 cctcatatgc ttccatccac tcatttgata taaccttatg tatagtcttt ccatctactc
    22501 tatttgaaat atatacagta ggagattctt ctttagttac tactgaaaaa gtaaaatcag
    22561 ctatatttgt agctactccc cattcacctt tattattcat ggcaactaat gataaatcac
    22621 cagcttttcc tcttcttttg ataagctgtt tatcaagttc atttactgca atatcacatg
    22681 cttcttgtgg atgcttccct tctttcataa gtctaactat ctcataagat atacatccct
    22741 tcattaaatc ttctcccaaa ccagtagctg tagctcctcc tatactacta tcaacataga
    22801 atcctgaacc agatagtgca gaatctccta ttctacctct tttcttcata aacaatccac
    22861 ttgttgatgt agctgcacac atttttccag ttttgtctaa agatatcata ccaacagtat
    22921 catgaccagc atatgggctt aatcctttgt ctaaagtctc tttttttctc tttttataat
    22981 gtaacttcgc tctatcagtc aacatatttt ttctttcaaa tccattttta tgagcataat
    23041 cttcagcacc tattccaact aaaaaattat ttaccttctc ataacttaac ttcctagcta
    23101 tgctgacagg attagaataa tctcttatac cagcaactgc gccaatatct aatgtgttac
    23161 catccataaa tgcagcgtca agttctacct cacaattttc atttggaagt ccaccatatc
    23221 caaccgattt atagaatgga tagtcttcaa ctcttcttac agcaagctct acagcatcac
    23281 ctgcttccat gcctttttct aaatgttctg atgcctcagt tattccttct agggccattc
    23341 tccatgtcgc tatcattccc cacatgttaa aattcccctc cttaaattat atagatttta
    23401 ctaatgtatg cttattataa atagtataat atttaaactt gctaataatt aatactctta
    23461 ttgataacaa aactcttctt tcatcaaagt tatatcttct ttatttgcta ttgcttttgc
    23521 caatttacat atgttattta atgaatcatt aagaccaatt ccaccttttt ttggagtctt
    23581 aactatacat atgtcatttt tatattcgtt tatcacatct atattttctt ctgacatagc
    23641 tgcaaaagct ggtatatctg ttttatcatt tatattttta gacagtattg ctgacatctt
    23701 agaaaagttt tcgtaattaa agcttggtcc acagattact acatcagggt ttaatttttt
    23761 taccatagct atcatcttac tcgttacttc ttcttgatta tttacaaaaa attcatcccc
    23821 acaaaataag gtagctatta ccttagattc atttaaaaat tgctccatca ttacacctgg
    23881 tcctaatggg cttgattttc cagcaggagg tatattactt ttctcttttc ctccagctcc
    23941 agcttgtatt tggtccaata tcataactat tttcttcata atcttcctcc tataatacta
    24001 aatcatcaaa agatatatca tcatcttcct cttcaacagt ttcattttct tctctcaagt
    24061 attgcttatc tagcattttt ataaatggta tatacataaa tattcctagt ataagaagta
    24121 ctaattgtaa gactgcacct tgccatcctg ttgttaaaaa accagagaat ataattggag
    24181 ttgtccacgg taactgaact ccatttgtaa gaggcactaa tcctgctgac atacagaagt
    24241 aagctattat tatatttatt gttggaacta ttgcaaatgg tatcaatagt attgggttaa
    24301 gcattattgg tagaccaaat atcaatggct catttatacc aaatattcct ggtaaaattg
    24361 atagttttcc cattgacttt atacgtttac ttttacatat taataacata gctatgacta
    24421 atgatagtgt actacctgct ccaccaaatg tcgcaaacat atcttggaat tgacctgtta
    24481 ttatgtttgg tatttttgaa ccagcttgaa aagcagctaa gttttcagct gatagaattt
    24541 ttaatattgg attatacaca gccccaacaa cactacttcc atttattcca aagaaccaga
    24601 ataaatgtaa gaatatataa gcgattgcca tagcacctaa actatttcct aattttaata
    24661 atggcatttg taaaaactta aatataaatt catgtatact tccatatggt gtagcttcaa
    24721 aagctatctt aactaaaaag aaaactacca taactattgc acttggtatt aatgctgcaa
    24781 atgattttga tacagttgga ggaactccat ctggcatttt caatgtccat ccttttttta
    24841 caactgcagc aaataatttt acagaggtta ttgctgttat catacctaca aacattccct
    24901 tagaacccat ccaacctagt ggtatacctg ttaccatgta aactgattct gttccttctg
    24961 gtgtatatgg tattacaaat ggtgttatta taaaaaaggc aactattgca actgctgctg
    25021 atgctattgg gtctacatct atttgctttg cataagaata tccaatccct aaaactgcaa
    25081 gtagagtcat tacttcaaat gttgctacag ttggttgttg caattttaca gcccaatctg
    25141 gaccaaaaat ttgacctaca aattcattcc atccttttat aggaaaattt gctactaata
    25201 aaaataatga acctattatt aataatggtg atgatactaa aaaaccatct ctaattgcaa
    25261 ttaataattt atttttacct attttttcag ccaatggcat aagtattctt tccaatctat
    25321 tcatctatcc accctcattt cctattattt tttactataa ttctacgctt gtttagattt
    25381 tattagcttt atagcaagtt ttaatacttt ttccccattc atagttccat aatcaacttc
    25441 attgattata tctataagtt ttccttgagc ttcaaacttt ggttttaact cctttagcat
    25501 gtatttaact tgtggtccta ataatataca atctggattt ttttcttcaa ctattttgtc
    25561 aatttctgct atcggaaatg cctctacttc tattggtaaa ttgtgttcat ctgctacctt
    25621 ctgcatttta tctgcaagta aacttgttga cataccaaag ctacaaaata aatatacttt
    25681 tcttttcatt ttttaaatcc cccttaattt ttttatttta atttttgaat ttcttttctt
    25741 aacataatca tttcttttat aaggtttctc tcactcatac aagtcattaa atggtcttga
    25801 gagtgaatca ataaaataga tatatctgat ttatctcccc cagcttcctt ttgtataagt
    25861 tcagtttgta ctttatgtgc ttttaaaatt tcttcatttg cattttcaat taattcatct
    25921 gcttttttaa aatcgttttc ctttgcatag tcaagtgctt cataaagcat agattttgca
    25981 tttccagcat gaattataat ctttaaaact atttcttcca ttttttcctc ctaagttgct
    26041 tctctacttt tagattattt ataccttaca aacatataat atcttataaa aacaaaaaaa
    26101 catgcacaac tttttccatc acatggaaaa ttttgtgctt atccatagtt cactatgaat
    26161 tttatataag aatatctttt accttttcaa tgtcacattt acttagaata tctagcatat
    26221 tttcatcctc tgttaaatta gatatttctt taaataattc tctaaatact tttctatcat
    26281 catctttttt atacgctaac aaaagtataa tctttatatc ctttccattt ctaattttta
    26341 ttggtgtttt taaccttgca aaacataaaa cagattgttt tatatatttc atgtctccat
    26401 gagggaataa tattcctttt ccaacattgg ttggatatga cttttctctt gttataatag
    26461 attgtaaata ctctttttct gcataatgtt tttcaattaa taaattagtt aaatatgtta
    26521 tagcttcata aatatcatca tgttctaact ctcttattaa ataatcagac atcattaatc
    26581 tgttcataag tttatatgaa gtgtcttttt cttttatttt gtttgtgaac tctttaagtt
    26641 tattctcatc ctcatcagta aataatggtg ttgttataaa aattggtttt tggatatttt
    26701 catactcttg aaatgcaatt ataaaatcta tagtcttatt aaagttatct ctttttaaat
    26761 cttcctctct taatgtttca ataattttta tgttatcaaa tctcttttct atctttactt
    26821 ttaatagcat acttactcca aaactaaatg gacatacaat acttgccttt ttatttgatt
    26881 cccttttgct gtattttctt tctagagagg tttgaaagtg aagtgtaaga taaccaattt
    26941 catcttccgt tatttgtttt ccttttaatt caatttctat tgaacttgat attactgtaa
    27001 ataaaaaaca aaatttagtt tttatgttat ctaaaagtgg attttctaag taaacattgc
    27061 ttttcatttg atgcattgta acattcaaat gacatattaa ttgttcataa agtaatatat
    27121 cattattaaa atttattcct gattcttctg atactaaatt tataattttt tttgtataac
    27181 ttgctaattc ttcatttaga tataaagatt tcttaatatt attactattt atttgtttgt
    27241 tcatacccaa tattaaagat tctataaaca atttttcttc ataatttaat actatggata
    27301 aattagattt caattcatct tctatatcca atataagcat ttttagctta agctctttct
    27361 tagtatttga tacttttaaa gaatttccca gccttattcg tattatattt ataagcaaaa
    27421 atagcatcaa ttgcttgaat gatatatctg taagtcttat atccatatcc tcttctatag
    27481 taagcattat gttttttaca attattatat ctactatatg gaagtactct atttcatcta
    27541 tgtttaatct gtatttatct atatatttaa ataagaatga tactaacatt attcttatgt
    27601 ctttctcatc cccttcaata tatataccac tgcctttttt agtcgagata tttaaattat
    27661 aattatttaa tgctactgat aatttactta tttcttctct gacaatattt ttatttatgt
    27721 acaacttatt agataaatca tttaaagtaa cttttttatt attacaaagt agtaaattta
    27781 gtatctcaac ttgcttatga agctcactat ttactctttt aacttcacct cgcaacttct
    27841 gatttaccag tattttgtct gttccatctc cactaaattt aatcccaata ttaggttttc
    27901 ttttgatata agcattacaa aaagtagata accattcatc aatatatttg caatcatttc
    27961 ttattgtcct ttctgaacac tcaaaatcat tggcaatctt attaacattt ataaactctt
    28021 cttttaatag caatgttgtt aataaatttt cttgtctttt attcaaacat atcacctctt
    28081 tatgactcaa cctaaattta ttatatataa aaaagagcta tacataaagg tatgctagct
    28141 ctcttttaat atgatattta gctgacctgg tttttgaccc cacactcgcc tactggaggg
    28201 ttcgcttggg ttcgccgcac tactactctc tctcgatttt attatcggta ggtcttactt
    28261 ctaagccgca ccatgatcat taacccaatt tttagtgtta acttacgtgg atccctttgc
    28321 ctgcgactgg cttggacttt caaccacaga cctaaatatc acttattcat tttaatattt
    28381 taaactaaaa ttgtcaatat ataagattat tccataatat aattatcatt tgcttttgta
    28441 agtagagtca atacaaaaac cttattagca cctgataact ttaaaagttt agaaatttcg
    28501 tttgttgtta gaccagtagt aaatatatca tctattaaaa gtatattttt attatttatt
    28561 aatttaacat tttctttaac aacaaataca ttttttaatt cttcttttcg ttctttctta
    28621 ttcaatttat atagcattct tgtatatttt tttctactta tgcaatctaa taatggaata
    28681 tttactattt tacttaaatt aaatgctatt ttttgtgcct gattaaaacc tcttttattt
    28741 agcctttttt tatgaagtgg aacaaataat atataatcaa attttatgtt ttctaaatat
    28801 aatttttctt tcattatttg agctatatac ttagccataa aggttttttg gttatacttc
    28861 agccctaaca ccatcttttt tgtcacatca ttgtattcaa tacaagatat tgatttatca
    28921 aaataaaagt ctttattaaa acaataacta cactcttcaa taaattcttt ttcaattgaa
    28981 tgacgtatta ttggttttcc acacttcatg caaccatctt gtataaaatt catttcttta
    29041 aaacaacttt tacatattga ataagtattt gttttcttta tggatttatc acaaattatg
    29101 caacttatat tctcaggata tataaaatcc aaacatttat taatcatatt tttaaaattc
    29161 ttaataaaat tatcattgca tattttcata tttattctat caacaaccct tcttgtttaa
    29221 atttatttag cttatatcct aaatttgaat acctttgatt aactctatta ttttttatca
    29281 tatattcaag atatttcaca tctccaacta atacaactaa tttttttgct cttgttactg
    29341 ctgtatataa taaattacgg ctaagtaaca taggaggagc ccacgctatt ggcaatacta
    29401 caactggaaa ttcacttccc tggcttttat gtattgttgt acaaaaacta tgatcaattt
    29461 catctaattc atcatatagg tatgatacaa ttttagtctg gtcaaataat atataaatag
    29521 ttttcttatc tttatctatg tgataaacat atcctatatc tccattatag ataccttctc
    29581 cactttcttt ttggtcttct gtttcccatt tttttgtata attatttttt acttgcatta
    29641 ccttatcgcc aactctaaat aatcttttag aaaaattttc ttcaacttta aatttttctt
    29701 ttttgttaag atacttctgt aactctatat ttagattagt aactcccaat tcaccttttc
    29761 tcattgaaga taaaacttgt atatctttta atttatcaac tttataaaat ttaggtaatc
    29821 tttcattaac caatcctatt atttcattta aaatttcttc attagttgat tttcttataa
    29881 aaaagaaatc tttacctttt gtatttagat aaagtggttc tccattgttt atcttatggg
    29941 catttacaat tatcatactt tcttgcgctt gtctaaatat ttcatttaat ttaactacat
    30001 ttatgatatt agaattaatc atatccttaa gtacatttcc agcacctaca gaaggtaatt
    30061 ggtcactatc tccaactaaa attactcttg ttcctaactt tatagctcta agtaaattgt
    30121 acattaagat aatgtctacc attgatacct catcaacaat tattacatct gaatttattg
    30181 ggtcttcttc atccttaaaa aaagttaaat catcatctgt tgaaaatccc atttctagca
    30241 acctatgtat agtttttgct tctttattag aagtttcact cattctcttt gctgctcttc
    30301 ccgttggagc tgccagagtc acacttttac cattattttc aaatatatct atgatagcat
    30361 taatggtagt tgttttacca gttcctggtc caccagttat aattaatact ccactattaa
    30421 cagattcttt gactgctaaa atttgttttt ctgctaactt aattttctta tcttcttcta
    30481 aaacattaat ctcgttatct atatctattt ttaagtcttc aaattcatac tgtgataatt
    30541 ttactatttg actacatact ccattttcag ctaaatagta tggcataagg tatataagat
    30601 tttcattgtt tactttctct atatgtattt tttgattata aactagcatc attacacact
    30661 cttttattat ttctccattt agctccaata atttttccga atcttgtatt agaacatgct
    30721 ctggtaaata tgtatgccca ttacttaaag atttatttaa agtgtataag attccctgac
    30781 aaattctatc tttagagttt ttatctatac caattttact tgctatacta tcagctactt
    30841 taaatccaat tcctcttata tcttcagcaa gttggtatgg gtttttattt ataacttcta
    30901 ttgcactgct tttatatttt ttatatattt ttaggcagta atttggtgtt attccaaaag
    30961 gagatagctg tattattata tttctcaatt ctctgtcttc ttcataactt tttactattt
    31021 gttttacttt tttactccct ataccttcta cttcttgtag tttttctggt gaattttgta
    31081 tcacctctaa cgtatctaca ccaaacttat caattattct ttttgccatt ttttcaccaa
    31141 taccatgtat cattccagaa gataagtaaa cataaattcc ttctaaggat gatggtgtta
    31201 ctggcataaa actattcact tcaaattgag tcccataaat cttatgattt acccactttc
    31261 cttcaacttc tatgctttct ccaattgcta aagtaggcat acatcctaca accacaattt
    31321 catcattttc atttaccagg tgagctattg tataaccatt atcttcgttt ttaaagacta
    31381 tttcacttat cattccttct aatttttcca tttttatctc ctaaaaatac taataatcgc
    31441 tccatttaaa tgttagtata attttatata ttaaaactat gataatcgta gaaaagtttt
    31501 atatttttat acttaacgta atttccaact attatacatt ttatcagaaa caaaaaaaat
    31561 ctgcatccat aaaatatggt accatgaaat acaggtacca tatttaaata gctattttat
    31621 tttaattact ctatatttag agtataaatt aaatttcata agtatattac agtactaact
    31681 tatttcagat ttattctcct tgtacccctc agttttagta attcttctat aaagcataca
    31741 tagtaaaatt aaaattccca tgtaacctgt atatggatat agtgtaccta ctaacttatc
    31801 aaatggcagt aaacctccta tacaagctaa tatacttacc actattgtta taattttaaa
    31861 cttagggtgg tcatcttcta caaatcggtt tgttactgac cataacaatg gtacagctgt
    31921 agtgtatatt cctagtaaaa gtactactga gaataaaata cctattattg gagatatctt
    31981 atcagctaag taaagtgctg gtatctcttt tgtgtatata tttcctatgt ctgaaagtaa
    32041 tgccaaattc atcattatag ctgctgccat aagtgctact ccacctacta taccacccca
    32101 tacagcatct ttcttattta ctgcagaagc tcccattcct gtcaagaatg ttattactat
    32161 tattacattg taacaagtat aaagtactcc cgagaaatat ccatttgatg tagcagtttt
    32221 aatatttaaa ctatcaacca tatttccagc atttgataaa gcatcaatat tgctaaataa
    32281 actaattgct cctactaaaa gtgtaaatat tattattata ggtcctatat tacctagtat
    32341 tttagaaagc cttgtcaaac ctaatgaaac agttattagg gaaacgattg ccataccaat
    32401 cctacctaca tatggattta atccatagta ctctgataat gtagctcctg ctcctgcaat
    32461 cattattact aatgttccaa ataaaaacaa tggaccaaac cattcaaaaa atgttccaag
    32521 atattttcca caatatactt gataaatttt tattggctcc ttaagtttca attctcttcc
    32581 cttatctata acttctgctc caaaccatga aaatagtacc atagagatta ttccacctat
    32641 aatacccgca tatccataaa acgtaaaaaa ttgcagtatt tcttgaccag ttgcaaatcc
    32701 agaacctatt acagtagcaa cataagctcc agcaaaactt ataaccgttt taatatttac
    32761 tttgcctgac atacactact ccccccaatt aaaattattt ttaaagtgta agtttattaa
    32821 gattatatta gactcattat tgtgatattt caatatattt ttaattattt aacattagtt
    32881 aacacattct taaccatcct ttaacatata tcccataata tgtataaatt tagtagagta
    32941 ttatataaaa tgtattgacc agattaaata ctgtttattc attatatgat gataaattaa
    33001 catttattaa aaatatttaa ctcataataa aaaagctgtt agaaagtaat cgtaaaatta
    33061 cattctaaca gctttttagt taaaaattat tcacctaaag catcttttct taactgctca
    33121 accttgtcag ttctttccca aggtaaatct atatcagttc taccaaagtg accataagca
    33181 gcaacctttt tataaagagg ctttcttaaa tctaagtctc ttattatcgc tcccggtctt
    33241 aaatcaaagt gtttatttac taattcaaca agtttttctt ctgaaacctt gcctgtccca
    33301 aaagtatcta taaatataga taatggtcta gctataccta tagcatatgc aagttctatc
    33361 tcacatttgt ctgcaagacc agccgcaaca atattttttg caacatatct agctgcgtaa
    33421 gcagcagatc tatcaacttt tgtagggtct tttcctgaga aagctcctcc accatgtcta
    33481 gaatatccac cataagtatc tattattatt tttcttcctg taagacctgt atctccttgt
    33541 ggtcctccta taacgaatct tcctgttgga tttatataaa ttttagtctc ttcatcaagt
    33601 aattctgctg gtataacttc cttaataaca tgattaatta aatcttctct tattttatca
    33661 tttgatactg tttcacaatg ttgagctgat ataagaactg tatgtactct tacagcttta
    33721 cttccttcat attcaacagt aacttgagtt tttccatctg gtcttaaata atcaactaaa
    33781 cctgtttttc taacttctgt aagtcttctt gataatttat gtgccaaaga tatcggaagt
    33841 ggcattaatt ctggagtctc attacatgca aatccaaaca ttataccttg gtctccagct
    33901 ccaacttttt ctatttcttc ttctatctct tctccagtct tactttctag accctcatca
    33961 actcccatag ctatatctcc agattgttca tcaatagaag ttataactgc gcaagtatca
    34021 caatcaaatc catattttgc tcttgtatat ccaatttctc ttactgtttc tcttactaat
    34081 ttaggaatgt ctacataagc agatgtactt atttctcctg ctactaaaac taaaccagta
    34141 gtaactgtag tttcacaagc tactctagat tgtgggtctt tttctaataa tgcatctaat
    34201 attgaatctg atatttggtc acatatctta tcaggatgtc cttctgtaac tgattctgac
    34261 gtaaataaat gtcttgccat ttttattccc ccttatttat tcttaatttt tattataggt
    34321 tttaccttgt ttttaatttt agaaatttac tcaaaataaa aaaacctctc ctttataaga
    34381 agaggttaat aatcgtaatt acatactaat taactgacct catctttcaa gacttttgtc
    34441 ttgtaggaat tagcaccttt tcccatttgg gtggttgctg ggcttcacag ggcctatccc
    34501 tcagcctctc ttgataaggt aatcaagatt taagttttgt tttactttac tagcttacta
    34561 tttttttaaa attttgtcaa tatttttagt catatttttt ctattccttt ttttgtaaga
    34621 caaataaaat tttctatcta tctgaaattt aaagttaggt ctttttaatg ctttttattc
    34681 atatattttt ttgaaggggg tgttcctatt gtatttagct aaagtaaact acagcgatga
    34741 tgatgttata cagatgctaa gttcagttct aaaaactata attaatgaaa atacgattgt
    34801 tgtttgcata ggaacagaca gggctatagg tgatacttta ggccctttag ttggaacaat
    34861 acttaagaac agtaacttta aataccccgt ttacggtaca ctagataacc ctattcacgc
    34921 cttaaatata tatgagtctt tagatactat aaaaaataca catatacaag gtaacttctt
    34981 ggctatagat gcttgcttgg ggtcgcagag taacataggt aatatacaga ttagagaagg
    35041 acctatcctg cccggaaaag gtgttggtaa aaaactacct caaataggaa attattctat
    35101 agtaggaatt gtagataaaa ttgatgaaaa taataaactt tcatttaata atatacgcct
    35161 tagctttata ttagatttag ctgaaacaat agccttggct ttattagtat ccacctaatc
    35221 taaatataaa ctaaatgata aaattcttta tataaaaagg tagatatttt agagtatctg
    35281 attaaatact ccaaattatc taccttttta cttttatatt tcaagttttt atttttctac
    35341 taaagcaaac attaattcac ctttacagac aagttcttca cctacatatg ccttagcatc
    35401 tgccttagct atattttttc taagagttgt catttcaact tccatagtta atgtatctcc
    35461 tggtcttact tctctcttga ttctaacttt atctatacct gcaaatacac ctagttttcc
    35521 tttgttttcc tctacagaca tcattgccac tccacatact tgtgctagag cttctactat
    35581 tagtactcct ggcatcaaag gatattctgg aaagtgacct tggaaaaatg gttcattaac
    35641 tgttatattc tttataccaa cagctctttt cccaacttct agctctgtaa tcttgtctac
    35701 taataaaaat ggatatctat gtggtattaa tttttttatt tcatctatat ttaacattct
    35761 aaactctcct aattattttc ttttgaaaga cccaccagtt tcaagtaagt ctattgaacc
    35821 taaagctatt cctgtacctt ttgcaacaca agataatggt tcatctgcta ttgtaacagt
    35881 tattcctgtc ctctgttcta ttattttatc taatccatat aataatgaac ctcctccagt
    35941 cattattata ccgacatctc ctatatctgc tgctaactca ggaggcgttt tttccaaaac
    36001 agcatgagta gcaactacta tttgctcaac acattctcta agtgcttcca acatttctgt
    36061 tgaatttata gttatagaac taggaagtcc tgtaattaaa tttcttcctg ttatcttcat
    36121 atatttttct tcttctctct taaatgcaga acctatttca aacttgattt tctctgctgt
    36181 tctttctcct ataagaagat tgtgttgttt tttcatatat ttaactatag catcatcaaa
    36241 tctatctcca ccaaccttta tagattcact tacaacagct cctcctagag atattacagc
    36301 tatatctaca gtacctccac caatatctat aatcatatta ccattaggct gtgatatgtc
    36361 tacaccagct cctatggcag ctgctattgg ttcttctatg atatatactt ctctagctcc
    36421 agcttgtctc gtagcatctt ctactgctct tttttccact tctgtgacac ctgttggtac
    36481 gcataccata attcttggca taaaaaatct tccaaatccc tttttatcaa ctatcttatc
    36541 tataaaagat tttaacatct tctcagttac atcataatct gaaattacac catctcttaa
    36601 aggtcttata gcgactatat tacctggtgt tctccctatc atctttttag cttcttctcc
    36661 cacagctaaa acagaatctg ttcttttgtc tatcgccaca acagatggtt cttctaaaac
    36721 gattccctta ccactaacat aaacaagcac atttgcagta cctaaatcta tgcctatatc
    36781 agctccagcc attttttcaa ctcctttatt tataatatta gattatatta taagcctatt
    36841 ttaatactcc tataataaag catatacaaa aatctcgctt taatcaagag aaaataaatc
    36901 ttttgagagt attattattt tgctatttat aattacttaa tatatttctt tttataattt
    36961 aattcaataa aaaaagtagg tttttaacct acattttttt atgttccttc tcatatttta
    37021 atttcgttgc taatccccct ctaatatgtc tctctgattt atttttgtcc agcacatttt
    37081 taacttcttt ggcaagggat ggatttattt ccttcaatct ttctgtaaca tccttatgaa
    37141 ttgtactttt acttactcca aatgtcttgg cggtttgtct tactgtagtg tttttttcaa
    37201 gtatatattt tgctaccact atggcccttt cctctatatg agatctcaaa gattaccccc
    37261 ctcacataat agctttaatg atatatatgt aaaaacacca aaaaatataa caagcttaac
    37321 caaatatgat taaacttgtt atactattac ttaattagac tcattgggtc tatagatttt
    37381 tctccactat acgcctctaa atgcacatgt ataccttctt cactttctat ttgagaagta
    37441 ctacctacag tacctagaga ttgtccttct gttaccttct gttctttctt aacactaaca
    37501 tttttatcta gatttgaata tactactaca atgttattgt cactttttat tttgactgat
    37561 tgtccatatt cttcatcatc aaatacatct actactattc cgtttaatag agattttact
    37621 tctttgcctt tagtacaact aatatctaca cctttatgag tttcccaaac atcaagtgtt
    37681 ttagaataac taggctcttt ttctgaatat cctcttatta ctttatctcc aatgtaattt
    37741 aattttgttg tactcgattt attttcctct tttgacttag ctttttctaa gttttgtttt
    37801 gaatccgttg cagtagggat agcatctttc ttgtcttttt caattaaatg tatttcctct
    37861 tcactatctt tattagcatt ttccataatt cctttattag aagccaattt atctacatta
    37921 ttatttgtaa accaaactcc acctactgct aataaacaaa cacatacaaa caaagataaa
    37981 taaaaaccat ctttttctaa cagctttttc ttcattttgc acctccaaat atatttctac
    38041 atttggatta ttgacgtgtt tttggcattt tatacaatta ttagtatata tcttttattt
    38101 ttgtatctgt gtagtaatgt gataatatat cataatattt ataaccctct tctgccattc
    38161 cttctgctcc ccactggctc ataccaacac catgaccata tccttttacc acgaaatcta
    38221 tgtatccttc tccaaatttt atgtcaaagt ttgcagagtt caacttaaaa acagttctaa
    38281 tgtcttttcc agtcaattct ttatttccta attttatttt ttctacagtt ccggcatcac
    38341 ttcttttagt tattgaaact tgtttactta aattattcac atctatcaca atagtgctat
    38401 atgcccttct aagactttta acaaaatctg tgttacttat tttaagtgtt gacgcaaatt
    38461 taggggaata cttatcataa ggactttcca cagactttaa ataaggatat tttgcagaaa
    38521 atacttcttc actattttct gtttttcctg aagatgtaga aaaataaaga ggaagtatcg
    38581 ccttatcatt ataagttatt atttgacctt tagttcctct aactgcttct tggattttgg
    38641 aatatttatt cttaatccat tcttcaccat ttaatttttt aagagtatca taactcttat
    38701 attcttgaca atgtttataa tcagtacata ctactgcatt tttatgttta ctagatttac
    38761 catgttcctg tttatatact acatatgttc tagctgctac agattgagct tttaaagctt
    38821 ctatatcaaa ctctgaagac atctcaccag ctagtactcc acataaatag ttctccatgt
    38881 ccatcttttc ggttttccct gttatatgat tatatacatt gataattggt gcttttttat
    38941 ttacagtttc atagttcact gtattttttt cttctttatt acctttgtct tcaatatcac
    39001 tttttttaat agttttgttt atagatactg ggctctctgg tttttcagtt aattctacat
    39061 ttttataaga aactaaagtt ataagtgatg gaactaacac agagcaagtc acaaatccta
    39121 acaaaacaac caatgggttc ttcatatact ctcctcctta gcttttaaaa ccttgtattt
    39181 aagtatatga acaaccctat taatttataa ctttcttcta taaaccaatt attaatcttc
    39241 tactatttcg atttgaccac ctagtgctgt tatcttctta tctatatcaa cataacctct
    39301 ttggatatgg tatatttctc ctatttgagt ttctccttct gctataagac cgcataaaat
    39361 aagggctgcc cctgctctta aatcagtagc gtttactgct gcaccgtgta gctcatctac
    39421 accattaaca accgcacttc ttccatctat ttttatatta gcacccattc tattaaattc
    39481 agcaacatgc ataaatctgt tttcaaaaac agtttctata actacaccag ttccattggc
    39541 tacagtaagc atagccataa attgtgcttg aacatctgta ggaaatcctg gatgtggtaa
    39601 agtttttata tctattggtt ttaatacttt tggacctaca actcttacag aattgtccat
    39661 ttctgttatt tcacaaccag cttctcttaa tttagctaca actggtttta aatgctccat
    39721 taaaacattt tcaacagtta tatcaccttt tgtcatagct gctgcaacca tataagttgc
    39781 tgcttctatt ctgtctggaa ttacattatg ttctgctcct tttaactctt taacaccttt
    39841 tatttttata gtattagtac ctgcaccttt aacatctgct cccatttcgt tcaagaagtt
    39901 tgctaaatct actatttctg gctcttctgc tgcattctct attattgtag tcccttctgc
    39961 taaagaagct gccatcatta tgttttcagt tgctccaaca gatgggaaat ctaaatataa
    40021 tttatttcct actaactttt ctgttgctgc ttcaacaaaa ccatggtcca tttctatctt
    40081 tgcacctaaa gctttaaatc cttttaaatg taaatctata ggtcttgttc ctatagcaca
    40141 tcctcctggc atagaaattt tcgtactatt aaatctagca agcaatggtc ccataaccaa
    40201 aaaagaagct ctcatctttc ttacaagctc atatggagct tcacaagttt ttatattgct
    40261 tgcatcaaca gtaagtgtat tttctttata ttcaacttct gctcctacat gtcttaataa
    40321 atctgatata acatgaacat ctcttaaatt tggtactcca tttaatgttg attttccgtt
    40381 tgctaataaa gtcgctgcta ttattggtaa aactgcattt tttgcaccat ctattttaac
    40441 actaccttta agtggattac tcttttttac tattatctta gccatatttt ttgatctcct
    40501 cataattaat aatctaattt tataattggt gtggcaatat atatgagtgt ttttccgtca
    40561 tcctcattat atttaattcc tatatttaaa ttaatttttt cccctaaata ttctaattca
    40621 taatcattta atactttact atatgctgtt atagataaaa agtttacgtc tttaacttca
    40681 tctatttgct tagcactcat attatataat attttttgta aaatatcatc atatttgttg
    40741 ttttgtaacc tttttgtgta ttctccaaca atacaagcag acacatctac ttgagatgaa
    40801 tatttattta acacattttc tacgttacta taaataacct ctttatgttt atatactttg
    40861 ttatccaata tgtcaactac tatgtaactt tctttgttat ttttatttat tccttttata
    40921 gaaatttctt tatcgctacc ttttatagta gcacataatt gtattttatc gccagaccct
    40981 ttttgattcc aatttattcc ttttttatcc aactttagat tgttaattat ttctataaaa
    41041 atattatgaa attctttatt tgataaatta tgatttataa cagaattaat tttaacatta
    41101 taaaatttaa aatttgcttc tgatatttca agagctttac ttaatctatt gtatctagta
    41161 tttatacttg tatctgcata agatattgtc attcctataa tcattaatat aaaaaagctt
    41221 atagctaatt ttactttttt catagtaaaa aatccccctt aaatatgttt ttctgtataa
    41281 tagaattatt aacaaattta agagagatat actttctcaa tatgtatttt tatacatttt
    41341 tgtatttttc atctaataat ctctttcaaa ctcttcaatc tcaattttct ctatccttct
    41401 cctggacttg ccaatttctt atatgtatca aatacatctt gcattaattc ttatatattc
    41461 tcttctgtaa tataaattac ttagtctata cttaaatcta taatctataa tctataatct
    41521 ataatctata atctattcat attttaccaa caaaataaac agataatact tttttatcaa
    41581 tttccatagc tccaaatgaa catacctaag tacacttccc aatacacaat ttacacactc
    41641 ttcctaataa atcaaaaaca cctttgtttt aaacaactct attgcacttt caaaaacttt
    41701 atttgtgttc tcccacttca attaaatttg aattatcaag actccagccc atccatccat
    41761 cactataaag ggaggtattt tccactccta tgacatttgc atatgttaat acttcagcag
    41821 cacgccatcc acttccacac ataaatataa gctgtttatt cacatctata ttttccttgt
    41881 cccacatttc taaaatctca tatttatttc tcattgtatt atcaatatta cgatattctt
    41941 ctagtgaaac actatcacta cctgaatgtc catataaagc tcctggtata cgcccttttt
    42001 tatcataata tgtatatcca gatacttttc ctatatgttc atcccaagta cggttatcta
    42061 ctagtataaa cttatttttt tcttttagtt tttgtcttaa ttcagatgta gtaacaatta
    42121 attgtgagtt aactggtata tctgctccaa aattagtaca tgaatgtttt ggattactaa
    42181 taaattctaa ttcatatcct gctgataacc atgagtttgt tccaccattc aaaactctta
    42241 catcttttac tcctatatat ctcaatataa cagctagtct ataagatgcc ataggtgtac
    42301 tacttgaaac tattacagta tcatcctttg ttaatccata gtctaaagca aacttagtta
    42361 agttatcatc attatcaagc atccatgttg gaggaggttc tataatatca gtatttacat
    42421 gaacacttgt aggtatatgc ccttttgtat acgattcttc tctccaactc gcttcaatca
    42481 tttttatatt ctttgaatct tcaaatgtct caggaatttt tccatctaat atatctttta
    42541 ttataaaagc aggaactatc atttgataat ttttatatct ttccattgga agattttcgt
    42601 cgtctgccca ttgtttaata tcatatttat atagatattt atatcctttt ttagataaat
    42661 aatctgctac aaccaaagca tcttttccat ttgcatcata caatactata tttttatcta
    42721 aatcaattct tttagttttc aatgcttgtt ctaacacttc atcttttcta tctgagtata
    42781 cacttaacca attagcagaa aaatctactg ctccttttat atgtccacct ctttttactc
    42841 catctaactt ccagccatta taagcatcat ttaagcgagt atcaactata acccaagcgt
    42901 tatcttgcac tttttcttgt aaaacttcag tagatattgc cttaagatta acaaaataat
    42961 cttctttctt ttctttttta aaatcatttg tcataattta tactcccaat ttaaaaaaat
    43021 ttttatttga aattatttta ctaaaataaa gtaattttct tatactctat tagcttcatt
    43081 atattctttt ataaatagtt ccttatattc ttattttaaa ctactcactt tatcttatca
    43141 caaggttact aataccttgt attttaattt ttctcataag ctattatttt tttatctgat
    43201 attgactata tgtttattaa gtactcacta tattttatat gaactatatt tatcgttccc
    43261 tatacctggt atggtctata tatttatcat tttacagatg atttaatatc aatcttttta
    43321 taaaatttat tatagcaaaa acaaaacaaa tttaatattt atctaaaaaa ctcatcctat
    43381 aaaatacaaa taacaggatg agttcttttc aataaatatt atctatttaa tttttaatct
    43441 gtttttattc aactgttact gatttagcta gatttcttgg cttatctaca tcgcatcctc
    43501 ttagtacaga gacatgataa gcaagtaatt gcattggtac aactgctaat attggtgata
    43561 aaatatcatc tgaatttggt atatagatta ttttatcagc agctttctca acatccttat
    43621 tatggctttg agctattgct actacataag ctcctcttgc tcttacttct tccatattag
    43681 aaaccatttt ttcaaataac ttttcttgag tagctatggc aattacagga gttccttttt
    43741 ctattaaagc tattgttcca tgctttagct ctcctgctgc aaaagcttct gcatgtatat
    43801 atgaaatctc ttttaatttc aatgaacctt ccattgctaa gctatagtct atacctcttc
    43861 ctaaatagaa tgcatgttca gaactaacaa ctgttttagc aacttctttt atatattctt
    43921 cattgtctag tatttcttgt atttttgatg gtatttcttt catcttagaa atcatactat
    43981 cataaaattc tctagttatt gttccttttt tgatagcaaa gtctaaagct atcatgtata
    44041 aagaagttat ttgagtagta tatgccttag ttgatgcaac tgctacttca ggtcctgccc
    44101 aagtatagaa tacatcatct gattctcttg ctatagatga acctacaaca ttagttattg
    44161 aaagtattct tgctccttta gcctttgaat ctcttaatac tgctagagta tctgctgtct
    44221 caccagattg actaactaat attactaatg aattttcatc tacaaatggg tcgctatatc
    44281 taaattctga tgctatatca gttattactg gtatatttac aaatttttct atggcatact
    44341 taccaagtaa tccagcatta tatgctgttc cacaagccac tatataaact ttatttattt
    44401 tttctaagtc ttctttagaa atatttatac tgtctagaat tatatttcca ttatcatcaa
    44461 gtcttctttc taaagtttct cttactcctg ttggttgctc atgaatttct tttgacataa
    44521 agtagtcata tccaccttta gacgcagctt ctacatccca agttacatga aatacttctt
    44581 tttcaacaag atttctatta gagtcataaa ctgttacatt ttcgtctttt agatgaacta
    44641 cttctccatt ttctaagaaa taaacatctc tagtatattt taataatgca ggtatatctg
    44701 atgctataaa gttttcacct tcaccaacac caactacaag aggactatct tttcttactg
    44761 caactaattc atttccatgt tctttacata taacacctaa tgcataagct cctcttaatt
    44821 ttgatatagt tttgtataca gcatctaata aattaccctc atagtattta tctactaaat
    44881 gtgctattac ctctgtatct gtttgagatt caaacttaac accttcagaa attaattctt
    44941 cttttatttc catatagttt tctattatcc cattatgaac aactgctatt gttttggctt
    45001 gattaaaatg aggatgtgag tttacatcag atggttctcc atgagttgcc catctcgtat
    45061 gccctatacc taaatttcca tcaataggat tcttttctaa gtcttctgca agtacagaaa
    45121 gtctaccttt aaactttcta atattaagtt ctttttcatt agaactatta actgctactc
    45181 ctgctgagtc ataacctctg tactctagct tagaaagccc ttctactata acttctgctg
    45241 cctttctact tcctaaatat ccaactattc cacacattat ttgtgtcctc ctaaaaatat
    45301 tatttatata ttttaagagt taggtcatat aagtttactt tggcttccct ttgtccatag
    45361 gattgctcct attatttaaa gaagtctttc gatggtaaac acaccgcaga gcatccgccg
    45421 acaattcgat taactctgac ctcgtcaact tagttcctat attactcaat aattatgtct
    45481 ataactaagt tctggcgctt agaaaggtgt cctcagtatt tatatctgag aatacccttt
    45541 aaattacgat aatttttctt gtataagatt agctaagttt gttgctaatt cttttatttg
    45601 accttcctct ttaccctcta acataactct aactaatggc tcagttcctg atggtcttat
    45661 caaaactcta ccatttccgt ctaaaataga ttctattctt tctatctcag tttttatttc
    45721 tggatattcc atatatttgt tcttattttc attttttatt gttgcattta ctaatacttg
    45781 tgggtattgg ctcattattg atgcaatttc tgataaaggt ttcttttctt gcaagattat
    45841 atttgcaagt attagagaac taagcactcc atctccagta gtgttgtaat ctaaaaatat
    45901 catatgtcca gattgctctc caccaagatt atatccattt ttaaccatat cttctaaaac
    45961 atatctgtct ccaactgctg tagtagataa atttattcca ttttccttag cagctattgt
    46021 caaccctata ttactcataa ctgtaactac caaagtatct tgtgccaatt tattattttt
    46081 ctttaaattt aaggcactca atatcattat atggtctcca tcaacaattt gacccttttc
    46141 atttactgca attaatctat cagcatctcc atcatatgca agacctaagt cagcattatg
    46201 ttccaaaaca gccctttgaa gttgttctgg atgtgtagag ccacatttat agtttatatt
    46261 atttccatct ggtgaactat ttattgaaat tacacttgct cctagttcat caaacactat
    46321 tggtgccact ttatatgacg caccattagc acagtccaaa actactttta atcccttaaa
    46381 gtcagtgctt attattgatt ttaggtagtc tacatagtct ctttgtgcac aatgttcatg
    46441 taattttctt cctacatttt caccaattgg aagacaatca attttgtcta tatcgtctat
    46501 atattcttct attttcaatt caatttcatc atctaattta tatccattct tattaaagaa
    46561 tttgattcca ttatattcaa caggattgtg agatgcagat ataaccacac cacaatcagc
    46621 accatatttt ctagttaaat atgccactgc aggtgttggt attattccaa cagtaataac
    46681 atcacatcca acagacataa gtcctgctat taaagatgct tccaacatat cacctgatat
    46741 tctagtatcc ttacctacta ctacttttac tctgtggtct ccttgagcta agacaaatcc
    46801 accagctcta ccaagtttat atgctaaatc acatgttagt tctgtatttg ctactcctct
    46861 aactccatct gttccaaaat actttctcac taaagattct cctttcataa ttgaaaagct
    46921 tgtaatttgt attatatttt tatagttcat aaaatatcat tataggctta atttcagtca
    46981 taaaaatatt ttaatactta tcagcaaaaa agacaataga aataattcta ttgtcttttt
    47041 tttgacatct ttttctttat tagttagttg ctttagaaca tgtagcatcc ataccttttt
    47101 gtaaagcttc tctgtttaat ggaacgaatt tttcttttct aggtccgaaa acttttttga
    47161 atgcttctag tacagattct atatctacag tatcagatac ttttaagtaa gcgcctaaca
    47221 ttatcatgtt agcaacttta tcatttccta attcagctgc taattcattt gctggtatat
    47281 aataaacatc tacatctgtt ctatcaactt tttcttttat taaagaacta tttactatta
    47341 tttttccacc aggaataaca tcatctttga atttttcaaa tgctggtata tttaataata
    47401 tagctgcagt tgcatcatct gttataagtg gagaaccaac tggctcatta gatacagtta
    47461 ctgcacagtt tgctgtacct ccacgcattt ctggtccata agatggtaac caagaaactt
    47521 cttttccttc tagcattcct gcataagtaa gtaattgtcc catagacata acaccttggc
    47581 ctccgaagcc tgcacatatt actctagctg tagacattta cttcacctcc tcaacttcaa
    47641 catttttgaa atttcctaat ggatagtatg gtatcatgtt gtctcttaac cattgtaaag
    47701 catcgtttgg agctagtccc cagtttgtag gacaagtaga taatacttct actattccaa
    47761 atcctaatcc tgcttgttga acttggaatg ctttctttat agctttttta gcttgtctaa
    47821 catgagctgg agtatcaact gctactcttt ctacaaatac agctcctgtt aatgtagcta
    47881 acatttcact tactcttatt ggataacctt gagtttcagc atttcttcct gattgagcag
    47941 tagtagctct ttgtccaact aatgtagttg gagccatttg tccaccagtc ataccataag
    48001 ttgtattgtt tacaaatata gtagttattt tttctcctct agcagctgca tgaactattt
    48061 cagcagtacc aattgaagct aagtctccat caccttgata agtgaatact gtattttcag
    48121 gatgaactct ttttattcct gtagcagcag ctggcgctct accatgagca gcttcttgag
    48181 tgtcacagtt aaaatattta tatcctaaaa ctgaacatcc aactggaaca acacctacag
    48241 catcacctaa tactcctagt tcttctaata cttcaccaac taatctatgg attataccat
    48301 gtgtacatcc tggacagtag tgagtttgag tatcttgtaa tccttcagtt ttcttaaata
    48361 caacagccat tatctagcac ctcctgcaac caattctccg gcaataattt ttttagcttt
    48421 ttcaactatt tctgctggtg ttggagtcat tcctcctggt cttccatgga agtaaactgg
    48481 taatttacct tctactgcca ttttaacatc ttccaccatt tgtcccatac tcatttcaac
    48541 agtcaatatg tttctagcat taggaatttg gttgaatgct tcgaatggga atggccataa
    48601 tactttaggt cttataagac ctgcttttat tccttcttct cttaatatat caattgcgct
    48661 ttttactact cttgaagtag ttccataagc tgcaaataca aattcagcat cttctgtttt
    48721 gtacatttcg tattgtactt cattcttttc cattgcatca aatttttctt gtaaatgcca
    48781 acaatggtct tctagtactt ctggctctaa gtatagagag tttattacat taggttttct
    48841 ttttccttta gttccaacag ttgcccaatc tttaggaggc aattctcttt tcttctcagg
    48901 agctctaaat tcaactggct ccatcatttg acctatcata ccatctgcaa ctaccataac
    48961 tggtgatctg taataatctg caacgtcaaa agcttccatt atcatatcaa cagcttcttg
    49021 aactgtagct ggtgcgaata ctggagttct gtagtctcca tttcctccac ctcttgtaga
    49081 cataaagtag tcagcttgtg aaggttgtat acttccaagt cctggaccac ctctcattac
    49141 attaagtaca acacaaggaa cttctgctcc tactgcatat gttatacctt cttgctttaa
    49201 agcaactcct ggtgaagatg aagaagtcat aactcttgct cctgcacctg ctccaccata
    49261 aaccatgttt atcgcagaaa cttctgattc agcttgaacg aacgctccac ctattttagg
    49321 taactctctt gataaatatt ctggtaattc actttgtgga gttattgggt aaccgaagaa
    49381 atatttgcaa ccagcttcta tagctgcttt tccaaatgct tcgttacctt tcataagtat
    49441 cttagccatt aaaattcccc cttaaatatt aattaaatta atctctttct actgttataa
    49501 ctacatctgg acacatagtt gcacaacttg cacatcctat gcatttttcc atttcaaaaa
    49561 cagttgctgg gttataccct tttttgttta ttacatttga gtctaattgt atgattttta
    49621 ctggacatgc ttcaacacac aatccacaac ctttacaacg ttcttgatta aaagatactt
    49681 ttcctttagc catttaccta agcctcctta aactttctat atatttgagc tcaaactaca
    49741 tccagtcttc tctcatatat aattttatag gaaaaatttc tccttctaaa tcttttggta
    49801 actgttcaac aagattttct aaacatgcaa catatcttat cggtatatta catttttcag
    49861 aaacttcttt tgcaactttc tgacctctta aaacatcatc aatagtagta aacctaataa
    49921 gatgagtatt attaattaga cctgttacct ttaatttaga agatttttct atttctttta
    49981 tatattggat aacttcttca gaagtttgag ttttctccct atttgcattt attacaaaaa
    50041 gcatatcata atcgccttct tttaacaaat gactaaatct tcctataacc ctagcaccta
    50101 cattatctcc acctaaatct ataacagaat tgtatgaatg gtctaccata ggagacatta
    50161 cctctgctga tactgctggt aaatctaatg ttggagcatt aattgagcta tctattggtt
    50221 gtatacctaa ggatttcatt aactctttct tttctctagt tctaaagtat acatttacca
    50281 catctaaatc ggctatagca actttgccag aaaccatatc tcttaactta acaacatagt
    50341 tcatcgcaaa ttcactttta ccacttccat agtgaccagt tatgattctt attcttttat
    50401 cattagttat cataaacttg agcctcttct tcaccagtta aaactctaag tccaccttga
    50461 gctaatgcaa tcatttcatc ttcacctgga taaactttta catctgctat aaatttaact
    50521 ctatctgcaa tcatttctgt aaacattttt gaatatgcga ttccaccagt taataatatt
    50581 gcttttacat ctcccttaag aactgcagca ctagctccta tttctttaga gatttgatat
    50641 gccatagctt catatgctaa tttagctttt tcatcaccag cttcaattct ttcttcaact
    50701 tctctagcat cattagtgtt taagtatgca actaatccgc cattaccttt tattttcttt
    50761 ttaatttcat cttgagtata ttttccacta aagcacattt ttactaatgc acctactggt
    50821 agtccaccac ttctttctgg agagaaaggt ccttctccat ctaatgcgtt tgcaacatct
    50881 actattttac catttttatg agctccaaca gaaactcctc cacccatatg tgcaactata
    50941 agatttatat cttcatattt cttgtttatt tctctagcat atcttcttgc tattgccttt
    51001 tgatttaaag catgtactac acttgctcta cttatttcag gcataccaga aattctagca
    51061 acatcttcta attcatctac aacaacaggg tctactatgt atgaaggaac atttacttct
    51121 tcacctattt gttttgctat tattccacct aggtttgaag cgtgttctcc taaaactccc
    51181 acttttaaat cttcaatcat agcagcactt actgaataag taccaccttt tataggttta
    51241 agaagtcctc ctctacctac tacagcatct aattcagatg tttttactcc accttctttt
    51301 agagcttctt ctattacttg tttacgaaat tcaaattggt cagacacctt ctcatatttt
    51361 cctatttctt ctgaagaatg tcttaaagtt ttttcaaata ctaaatcctc attatcaaat
    51421 acagctattt tagttgatgt cgaaccagga tttattgtta agattttaaa tattttgctc
    51481 atttgcctat ttccccctta aaattatgcc tttgctgcca ttaaaacacc taaagctatt
    51541 gagtttagtt tagtttcttc actgtctgct ctagaagtta atattattgg tgctttagcc
    51601 ccaactataa ctcctgcatt ttttgatttt gagaagaata ccaaagcttt atataatatg
    51661 ttaccacctt caatatctgg ggctaataat atatcagctc ttcctgctac aggatgattt
    51721 atacctttat gtttagctgc ttctaaagat actgcattat caattgcaaa aggtccacca
    51781 accatacaac ctttgatttc tcctctttca tacatttctt ctagttcttt agcttcaact
    51841 gtatctttca tttttggatt tactttttct tttgcgcata ttgcagcaac ttttggttca
    51901 cttatatcta atgaatgtgc tactgtgcaa gcattttcta tgatttgctt tttagtattt
    51961 gtatcaggag ctaagttcat agctgcgtca gttacgaaaa ataatctatc atatccctct
    52021 acatcaaata ctgctacgtg acttaataca tttccagttc taagacctac ttctttattt
    52081 aaaactgctt ttagtattat tgatgtgtct actaagcctt tcattaccat gtcggctttt
    52141 ccttgtgaaa ctaattcaac agattttaga gatgcttctg ctaaatcttt tatatctatc
    52201 agttcataat tttcgatatc catgtctatg ctttttgcaa tttctttagt cttttctata
    52261 tctcctacta aaatggcatt tgctattttt tcttttctag ccatttcaac tgccattaaa
    52321 acttctttat cttggcaaca tgctactgat atagttttag gtcctctttc ttttgcaaac
    52381 ttaattactt cttcaaaact tctcatgcat ttccacctca tcttgaattt tttgaaatag
    52441 aactttttat atctcaatta aattataact cgttcagcaa cgttttccta gagataattg
    52501 aaatatttaa ttcaaaatat ttatttaaat ctatttatca gtcttttttc atatatctag
    52561 cgctttttag atttaacaaa tatctcattg tccttatttt ataatataac taaaataaac
    52621 aagatgcaac aaaaacataa aattaacaaa cttaatttta taggtttttt tgtcgcatct
    52681 tgtaacaact aaatattcga aatatttgtt cattccactt caacaatatt aggttctata
    52741 tttatttttt taaattgata tgttgtttca tattttattt tatatttact atcttcctca
    52801 ggaccttgtg ataaatcaat atataatgaa atatcttttt tatttaactt actcaaatcc
    52861 tccattgact ctacaacaac ttttatatct acatcagaaa ttacatttgg attatccata
    52921 tttgttggga tatttcttaa ttcaatatct cccgcattat ataaaaattc tgcactcaat
    52981 tgttttgtat catttaattt aattgttata gagctatcct caagagttat accctctgga
    53041 atttctaagc taacttcttt ttcttcacca ttagccaaat cagctaagtc aataggtttt
    53101 gtcttaatat aacttatttt gtctatatct tctttctttc cttttattgt aacttgttcc
    53161 ttacttaatt tatagttttt cagtacatca ttatctttat tatcatttag acttactctt
    53221 attgggacaa ttttttcctt caatagttct atctttgcag tcacaaagga tgaacttaat
    53281 gttactcctt caaccttagt tccatcacta tttaatggta ttaattgcaa ttttttagaa
    53341 aaatcatctg tctcattacc aacatctaga ttagctctta cacttttgac ttctttaact
    53401 agagttcttg gacctgatac atcgacaaat tccttactaa gttcaatttt atttatattt
    53461 ctttttgaat ttccacttac ttctaccttt atatctcttt tttcttttat gtttttttca
    53521 agatttacta ctaaaacatc atgcttaaat tctagagtaa caccttgagg aatatttgct
    53581 tttaaatata cttccttctg tccttgtatt gggtcttcaa tggttccata tacatgtata
    53641 tcttcttttt taacactttg tacctttgat aaactacctt ttatatatac atctgctgtt
    53701 aatttttcgt ctggatatat aacaagattt ttatccttta aatcactcat attacttata
    53761 gatacaggta tatcttcaaa ccctttagtt tcttcagggt caactacagc cataacatac
    53821 atccataatg caattgcact taaaaatgat attattttta ttttggtatt attttttagt
    53881 ctatttatca tttaaatata ccacctttga aaaaacttct tgtttctgta tttgttttta
    53941 aattactacg taatatattc atcattcttt ctctagaaat atctctatat aacttacctg
    54001 ctttagcaat tgatacgcca cctgtttcct ctgatacaat caaagttata cagtcagaca
    54061 cctcacttac ccctattcct gctctatgtc tagtacccaa atctttactt aaatccttac
    54121 tttctgtcaa tggcaaaaaa catgcagctg cttttacctt agaatctcta attacaacag
    54181 ctccatcgtg taatggtgta tttggtataa atatatttat taatagttgc ctagaaattt
    54241 cagcatcaat aatagttcct gtatttatta tatcacttat tctagtttct ctctccatta
    54301 tgataagagc tcctattttt tgtctagaaa gtgaatataa agcttctaca atttcttcta
    54361 tatttctatt gagagtttct tcagaagtat ttacattctt tccaaataaa ttaaactttg
    54421 ctcttcctat atgttcaagt cctgctctaa attctggttg aaaaattata agagcagcta
    54481 taactccata atctaaagct ttaagagata tccaataaac ggtatgcaat ttaaaggtgt
    54541 tactaagttg agttgctaat aaaagaaata ttataccttt aaacacttgt tctgctcttg
    54601 tatcttttat aaacataaat attttgtaaa aaatatacgc tactatagat atgtcaatta
    54661 aatcatatat actcattctt aatattatat taaaaaatcc atttaaaaaa gaatttatat
    54721 ctaacacagt tttatcctcc tgtttgataa cttcttaatt atttacacta agtattatac
    54781 tatataaatc taactctata caaactattt aacttttatg taaataattc tagttttata
    54841 tttggagatt gttaaataat ctctaaacac attaaaaata tcaacttaat aaatacatat
    54901 ttcttattaa agttagtttt tactcaaata actcaaacca tcaaggcgcg aatacaaaat
    54961 agtactcgcg ccttaattca gttttatgtt ctaaaatatc ttaatatatc tttgtttaat
    55021 atatattaat tattataaat tctataatcc acttattaac tataaacaaa tacaaaatct
    55081 aaatatt
//
LOCUS       DAMJVK010000013        51338 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.013, whole genome shotgun sequence.
ACCESSION   DAMJVK010000013 DAMJVK010000000
VERSION     DAMJVK010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 51338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51338
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.013"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            99..1094
                     /gene="ilvC"
                     /locus_tag="PC414_000805"
     CDS             99..1094
                     /gene="ilvC"
                     /locus_tag="PC414_000805"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HDF2463214.1"
                     /translation="MKMARMYYEKDVDLEVLKNKKVAVLGYGSQGHAHAQNLRDNGVH
                     VVIGLYDGSKSAQKAKEDGFEVKSVAEATKESDLTMMLMPDEKQKKVYEESVKDNLKE
                     GQTLAFAHGFNIHYNQVQPPEFVDVVMVAPKGPGHLVRNVFTKGSGVPALFAVYQDYT
                     KKATETVLAYAKGIGATRAGVLETTFKEETETDLFGEQSVLCGGISELIKLGYKTLVD
                     AGYQKEVAYFECLHEMKLIVDLIYEGGFERMRYSISDTAEYGDYVSGKRVITDVAKQG
                     MQNVLEDIQNGKFAKAWIKENEEGRENFLKTREEEYNTEIAEVGRNLRSMMSFLK"
     gene            1147..2835
                     /gene="ilvB"
                     /locus_tag="PC414_000806"
     CDS             1147..2835
                     /gene="ilvB"
                     /locus_tag="PC414_000806"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HDF2463215.1"
                     /translation="MNGAKVILECLKKEGIDTIFGYPGGAVIPLYDALYDYSDDFKHI
                     RTSHEQGLVHAADGYARSTNTVGVCFTTSGPGATNAITGIATAFMDSSPMVVISGQVP
                     TSLLGKDSFQEIDITGATLSMTKHNYLVRNTKELVPTIKEAFRVANSGRKGPVLVDVP
                     KDLFLAEMDFSGEDYDLCQIDDYMDYKSDFDLDDETNIKLLNEAIDIIKESKKPIIYA
                     GGGVKSSDSEEILEKFATKIDTPVLNTLMGLGNIDRKNELSLGMVGMHGSRESNLALS
                     NSDLVIAIGARFSDRVISKSSEFAKNAKIIHIDIDPSEISKNIESNVSLVGDVKLVLS
                     LLIERVESKNNSNWKEEIKRFRKSEGVQTYEFHPQNILKKINEKYETLKKPTVVVTDV
                     GQHQMWAAKYWNFKGNKSFITSAGLGTMGFGLGAAIGTKVGNVDKNVVLVTGDGSFRM
                     NCNELATVANYNVPMLILLLNNRTLGMVRQWQKLFSNQRYSQTDINENVDYVKLVNAY
                     NIDGYKVSSMEELGKALDMIDFNKPVFLQCDIDKDYDVYPIVAPNDALENLICN"
     gene            3046..3723
                     /locus_tag="PC414_000807"
     CDS             3046..3723
                     /locus_tag="PC414_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HDF2463216.1"
                     /translation="MFKHDKALLKEVKVERPNPQYAVLMQEQLGGANGELKAAMQYLS
                     QSFRIKDPQIKDLFLDIAAEELSHMEMVAQTINLLNGHDVDYNAVNTGEIETHVLTGL
                     SPVLINSSGAPWTANYVTVTGDLVADLLSNIASEQRAKVVYEYLYRQIDDKYVKETID
                     FLLNREEAHNALFRDALNKVKDTGSNRDFGVTEDSKLYFDLSTPGPNHDTKIDINPPS
                     FEKPLKK"
     gene            3892..5928
                     /locus_tag="PC414_000808"
     CDS             3892..5928
                     /locus_tag="PC414_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HDF2463217.1"
                     /translation="MDSNRKPNNLINEKSPYLLQHAYNPINWYSWNDEAFKKAKEEDK
                     PIFLSVGYSTCHWCHVMEKESFEDEEVAEIMNRNFVAIKVDKEERPDVDSVYMTVCQA
                     MTGSGGWPMTIIMTPDKKPFFAGTYFPKYSRYNRPGVIDLLENVSEKWNTSRDILIKS
                     GDEIIEALKDDFGVKNTEGDLSKEMLSSSVRVFKAIYDEKYGGFGNAPKFPSPQNLMF
                     LMKYYSIEKDKDVLKMVEKTLDGMYRGGLFDHIGFGFSRYSTDKKWLAPHFEKMLYDN
                     AMLTIAFLDAYKITKKELYKEIAIKTIDYVVREMKDKEGGFYSAQDADSEGEEGKFYT
                     FNPLEIIEVLGEEDGIFFNNYFDITSSGNFEGKSIPNLIKNKEYERHNEKIADLSKKV
                     FEYRKERTSLHKDDKILTSWNALMIVALTKAYSTLKNDIYLEYSNKCLNFINNNLVNE
                     SGRLLARYRDGSSDYLAYLDDYAFLIWAYIELYESTFNMKYLEKALNLNESCINLFWD
                     YEKSGFYIYGKDSENLIARPKDLYDGAIPSGNSVQLYNLIRLAKITGDNRLEEMSYKQ
                     LKLYVDNVKSSPAGYSFYMLSLMFELYSTKEIICIFKEDSDLIAFKELISENFIPNAT
                     FLAKKYNEENTIIGFLNNYRLKDDKISYYVCQSNSCSQPINDLQKLKDMILGVE"
     gene            complement(6119..6685)
                     /locus_tag="PC414_000809"
     CDS             complement(6119..6685)
                     /locus_tag="PC414_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2463218.1"
                     /translation="MDYSNELKELFLMNQTYATLFTLTNKIQIEGDKYFGILTSRQYM
                     TILSILHLPEEETTLNNIARKMGTSKQNINRLVANLEKNGYVDVIPSPHDKRAINVKV
                     TDLGKKVMVTCSRTGINFMADVFHEFTKDELETLWSLLKKMYRFNGEEQDGFEEDANF
                     MEYEEIDKIKSEALEEFAKRRKRVNKND"
     gene            6973..7908
                     /locus_tag="PC414_000810"
     CDS             6973..7908
                     /locus_tag="PC414_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2463219.1"
                     /translation="MKCTNFTFKGEEGLDIYTYKWEDENIKKPKAVIQIAHGMAETAQ
                     RYETFAKVLTKNGYIVYINDHRGHGKTAKIIENVGHLAEKEGFRCLVEDMYTLTNIIK
                     KENEDLPIYLFGHSMGSFASQRYIMDYSNNLSGLILCGSNGKQGIILNLAHLIINREI
                     KKYGRRSKSNKINNLIFGGEIIRRNEKTKFDWLSRDKEQVEKYINDPFCGVVCSCGFF
                     YDLVQGLKEIEDKENLKKVPLDIPIYIISGDKDPIGKNGKGVLRLRDRYIKLGVKDVT
                     CKLYKDGRHELLNEINREEVFEDIICWLNNKIEIL"
     gene            8373..9587
                     /locus_tag="PC414_000811"
     CDS             8373..9587
                     /locus_tag="PC414_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2463220.1"
                     /translation="MQIKIGKVIQCLRKERNLTQEQLAKFIGVSTPAVSKWESGNSYP
                     DIELLPLLADFFNVSIDKLLNYKIDLSEEEVMKIYKELESGFARIEIDLSTEEPKEEF
                     RQDLESVKKLSNMYIEKYPKSYLLKLRICSLYQMYSYKFGKSELNDRVKETTNILEDI
                     VRNTDDIQIKETALIILSNAYCMLEDYEKAELYLNMIHKSIGDTSVNLAMIYLKQNRL
                     EEAEILLQNKLFSNVFNISMDCKGIINVYKNQYKELKKKLENRNFNKNAIEREMEYIK
                     NKLLGYANLSLEIKKMLSEDKGAFFSMYMDYMELSLIFLFFNMKEEAKKALYSLKEIL
                     EKYPIHESLDVSQMRFFDKVESKNLYTFNIYTNLLIVLNDDSYNELREEPIFKDVIEK
                     ILDMEKMLKNKE"
     gene            complement(10007..10528)
                     /locus_tag="PC414_000812"
     CDS             complement(10007..10528)
                     /locus_tag="PC414_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2463221.1"
                     /translation="MKFERLRTSENKLYYKAMELYKISFPFHKQRKSSLQAEILKNKE
                     YQFNLIYDKNQFVGIILCWETDNFIYVEHFCIFPEIRNNRYGQRALELLNKKGKTIIL
                     EIDLPIDEISIYRKAFYERVKYKTNNYEHIHPPYHKKFNGHNLIVMSYPEKLSKMEYD
                     KFNHYLRNNVMSF"
     gene            10884..11681
                     /locus_tag="PC414_000813"
     CDS             10884..11681
                     /locus_tag="PC414_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HDF2463222.1"
                     /translation="MEKREKRIIISSLLSVSILMGLASIYSILNKEDISLTVKGQEQK
                     VSSFKKTVEELLDEQGVKYNSEDKINPSLDTELKDDMKIKVVKVTKSKKEEIEKIPFD
                     TKHVNDSNLLKGKSKVYQEGQEGEKKLVYNLTYHDGKLVKKVLSKEVISKEPTTKIIK
                     YGTKEKVLIASRGANIRGGKHMKVVATAYAGDTITSTGTTPRWGVIAVDPRVIPYGTK
                     VYIPKLGMTFVAEDCGGAIKGNRIDIFMNSEGKASNWGRKSIDIYLH"
     gene            11791..12381
                     /locus_tag="PC414_000814"
     CDS             11791..12381
                     /locus_tag="PC414_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDF2463223.1"
                     /translation="MKIVCLGDSLTYGFGVSRSNSWTNIVNKETRLEIVNKGINGDTT
                     SGMLARFNEDVVKNSPDIVFIMGGTNDFIAGAGNEIINSNIMAMVHQSYSKNIIPIIG
                     IPLKPDIPNVREDWSCFTDFNIVSQNLESYSYWIKKFCMTFNTNFVDFYSEYNKNMEL
                     EGYKKLYFDGLHPTKEGHRIMADIFINSINKYIEEL"
     gene            12447..14144
                     /locus_tag="PC414_000815"
     CDS             12447..14144
                     /locus_tag="PC414_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HDF2463224.1"
                     /translation="MDYTKTYEEWIKGSYFDEDTKLELENIKNNEKEIEDRFYKDLEF
                     GTAGLRGIIEAGTNRINKYTVRRATFGLANYILENTTKEETSRGVVIAHDNRHKSRQF
                     CIESANTLAACGIKAYIFDSLRTTPELSFAVRSLNAIAGIVITASHNPPEYNGYKVYW
                     EDGAQVMPEIANAITEKVNSIHDYSTIPTLTEANKNLVVLLDESQDTKFIEAVKSQII
                     RKDLVKNVGKSFKIVYTPLCGTGNVPIRRALKEVGFENIIVVPEEENPDPNFAGLDYP
                     NPEEKKALNRGILLAKEKGADLVIATDPDCDRVGVAVKTTTGEYALLTGNQIGGMLTH
                     YIIEGLKENNKLKENPTIIKTIVTSEFGADIAKANNVDVLNVLTGFKFIGEKIKLFEQ
                     NKNRSYVFGYEESYGYLVGTHARDKDGVVSSLLISEMAAFYYSKGMSLYDGLIELYKK
                     YGFFKEQTISLTLKGIEGVEKIKEIISYFRENQIDYINSIKVVDKKDYKNGIDNLPKS
                     DVLKYFLEDESWVAIRPSGTEPKLKFYIAVKGASDIEADKKIQGLKKYIDDMVEKLK"
     gene            14358..15197
                     /locus_tag="PC414_000816"
     CDS             14358..15197
                     /locus_tag="PC414_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HDF2463225.1"
                     /translation="MFGIDKKLIEYIIAKSNYSELSSKSAEVSFFLMLSIFPFLIFTI
                     SSIAYIPILHLNKYIALFRNMMPEGAFAVLSSIIVSAIDNRNLKFLAVSFVLTMWTFS
                     RAVKALIKGMNRAYKVKETRSFFKILSISFLFTIMLLVLIFLSMIFLVYGEKIGYFIF
                     NLVGLDEIFIKIWDILRYTVGIITIIVIFTLLYKYTPNKKLTIKESAPGAIFATFAWF
                     LVSFLYSYYTNYYANYEVIYGSIAEIIVLMTWMYFSSWSIVIGYEVNSRLYFRKIRHE
                     MLK"
     gene            15714..17570
                     /locus_tag="PC414_000817"
     CDS             15714..17570
                     /locus_tag="PC414_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HDF2463226.1"
                     /translation="MDTHNKYVNFIKNIPVPFLYCRIVKRQEDIEYRVEYISKGMGKV
                     LQLEEGICDKNILDVLPVFKSKKYFKELFSNEVDCIKRYIPTLKNWINIKKQIIGDSY
                     IILYFGKIVFDYRQIIDSFDKKEKVAYIKDEEGTYIDCSENLIPILNNNIKTTKDIFG
                     KNDIEVWGENTGKLFRDDYREGVSSKKRFLQNLFEYEETFFMVEKYFLYDEDELLGTI
                     GIVDNIIYSGYSNRNYNSKDLMKMIEHSIPENMFYKDVYGNYIGFNSGFLNLACMNKE
                     ELLGKNSYKISEEEALIDKIFESDKGVVENKKVVTFELNISMNDENKCIEITKRPFFD
                     SYGSVIGIIGTARDISRRKRLEEEMDKTRMEFFANLSHELRTPINLISSSLQVIEKKE
                     ADLIESNDTLKRNLGIIKQNGNRILRLVNNVIDFTKMQSGYLDFKPEESDIIAFIEEI
                     CMSVADFASQNNIQLTFDTEIEEFSMLFDSEKLERIILNLLSNGIKYNKKDGKINIFL
                     YVKDNVFNMKISDSGIGIPKEKIDKIFNRFEQIDNELSYRVKGSGIGLSLVKSLVELH
                     EGSISLKSQLGIGSEFIVSLPVRSKNNIEKYNHKREISNELSKKLEIEFSDL"
     misc_binding    17653..17893
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    17959..18194
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            18279..19496
                     /locus_tag="PC414_000818"
     CDS             18279..19496
                     /locus_tag="PC414_000818"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HDF2463227.1"
                     /translation="MENKVKIGVLGYGVVGSGLIDIIDNNKEKRNIEIVGILVNNLEK
                     HKDKKYSNIITNNIDDIFNKDIDILVEVMGGLEPSLSYIKKALNNKIHVVTANKDLLA
                     ECGDELAKLASENKVSIKFEASVAGGIPVLKPIIESLEGNNIDSINAILNGTTNFILS
                     KMYDENLSYDMALRQAQELGFAEANPESDVLGYDAARKLSILSTLAYDNRVYWKDVYL
                     EGITDIDEKDIEYAKKLNCKIKLIGQSKYENDKVSAFVRPVLVEKDNILARIDNEFNA
                     VIVNGDSVGEVSFVGKGAGSLATGSAVYSDVIDIIDNRVSSIDSFTKDKIQVNKIVRE
                     KCGALLRFKKCNKDEILNIVENCLVKFDILNDDDELAIMVYADSEYEINNSLCLIKDK
                     GYCEKMNKMLKIS"
     gene            19613..20095
                     /gene="cdeM"
                     /locus_tag="PC414_000819"
     CDS             19613..20095
                     /gene="cdeM"
                     /locus_tag="PC414_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeM"
                     /protein_id="HDF2463228.1"
                     /translation="MENKKCYSEDWYERGESTAKWFQNDREEYEREAYDEDRERRGSN
                     CGCSDSGENRPRNCERFRREAEIREREAREAFCESSEKKKEALAYECEARKLWEEAEK
                     YWDEYSKYNYKGIEYLAEAARLFDEGMECEARRNGNNGGNNNNCCHKCHKCNCNCCRK
                     "
     gene            20359..21624
                     /gene="hisD"
                     /locus_tag="PC414_000820"
     CDS             20359..21624
                     /gene="hisD"
                     /locus_tag="PC414_000820"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HDF2463229.1"
                     /translation="MKSIKTSSMKEKVDEKILREQVSSIIEDIRNNKDVALKKYNEKF
                     DRNTRDEFRITKEEIKEAYKHVDDEFINNLKIAAKNIREFAKAQKLSFENPFEKEIYP
                     GVILGQTNIPIESCLAYVPGGQYPLFSTALMLIIPAKVAGVKRIVACSPTMKNTEKIN
                     PKTLVAMDIAGADEIYATGGVQAIAAFTYGTEKINPVDIIVGPGNKFVTEAKRQCYGQ
                     VGIDFVAGPSEVLIIADETSNPVYIAADLLAQCEHDLNARGILLTNSLEVAQKVEKNI
                     ESMLKDLPTKDIAYSSWENNGEIILVDDMEEAIKISNFYAPEHLEVAVNEYNDICDRL
                     TNYGSLFIGNLSAEVFGDYVSGTNHTLPTLKASRYTGGVWVGTFIKTCTKQIFNEKAI
                     QSLAPVAEKLAKEEGLYAHAKAAEVRFKK"
     gene            complement(21827..22579)
                     /locus_tag="PC414_000821"
     CDS             complement(21827..22579)
                     /locus_tag="PC414_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2463230.1"
                     /translation="MSYFQYQGNSCFYKEYGQGKPIIFLHGNTGSSNMFKALVPLYAE
                     NFRCILIDFLGNGQSDRVSQFSPDIWHDEALQTIALIEHLNCGKVGLIGTSGGAWAAV
                     NTGLERPDLVEAIIADSFDGRTLNDNFIDNLITGREKSKQDIQARKFYEWCHGKDWEN
                     VVNLDTKALLQCANEKRPLFHKELCKLEMPILFTGSKEDEMCRHNLEEEYKQMATFIS
                     KASIYIFSQGGHPAILTNADEFAQLAKVFFKY"
     gene            complement(22697..22966)
                     /locus_tag="PC414_000822"
     CDS             complement(22697..22966)
                     /locus_tag="PC414_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463231.1"
                     /translation="MSYQNSIDLLPKELIEQVQEYIDGKVIYIPKKQEHKKHWGENTN
                     TKQVLASRNSQICINFQNGMSIKQLSEKYFLTEKSIQRILKQQNL"
     gene            23441..24535
                     /locus_tag="PC414_000823"
     CDS             23441..24535
                     /locus_tag="PC414_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDF2463232.1"
                     /translation="MKNIVGKPQTIKKVNEDLIKNIIKNEGPITKPEISSITELSLAT
                     VNKIVEQLALKNEVKVSGLSESTGGRRAQLFEINANLEHIIALYYYRNCCIGVVANLL
                     GEIIYQEEFNIRMDTYENVNQDTFFVIDTLIKKEKGNNIRAIGIGVPGVVKKGVVKNI
                     PTIKSWEGINLENLIKSKYNTKVFIENDTNITTIGIYQKQYKDKYKNMALMYLEDGIG
                     SGIVINGELYLGSTNFAGELSYLNIEFGKYNSNNLNLEDYIMSLRKRYLESKDNKFKK
                     EILSIISNFIRTLVCVLNPQAMIIQCSILTKSDMEFIEKDVSNNVGEENFPKLIKVDS
                     LREGSINGVISMCLKETDYQYSLSNKKGGY"
     gene            24537..25487
                     /locus_tag="PC414_000824"
     CDS             24537..25487
                     /locus_tag="PC414_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HDF2463233.1"
                     /translation="MKYLVSIDGGGTKTKFCVSDLDGNILKEHTTGSTNYKSVGIKKT
                     YENINNGFKKILKDLYIDYDDIEYTVFGISGCDSPNDYKIIMDEILKIGINKEKIYLA
                     NDAVLAFYAQADSPGLVIVAGTGSIILGIKEDGEIYRVGGWGYNFSDLGSGYDIGRKL
                     LKKVLLYCDECHEYSDLFSCVLDFFGANSFEQLAYMITDINDNVEIANLASLVIDCAE
                     QGDKLAIEILRESSTELSKLAQVILRKISNPMKMDKSEINIVLSGGTLNKTVYAQMLM
                     NNLKTENTDKNLKFISQENQPVYGGIRLAMALANRRVKHG"
     gene            25480..26664
                     /locus_tag="PC414_000825"
     CDS             25480..26664
                     /locus_tag="PC414_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463234.1"
                     /translation="MDRKKVIVQIFTGGFKAKEIKFNDIKCKLLELLDCIDIEKVIMG
                     WSVDEELYVETKKLLNKYNVELYLWLPCFSEIGLLEKSNLLIDYMGKEVKGYALQEDE
                     NFEFYCPNNKENIKNIKKIYSTYFSDIKFDGIFLDKIRYGSFSNKLNGVFNCFCKDCL
                     NIYKKNNIDKESLMREMNNVSKGVIDNEDTPFGITEYINGKYKFKNKIWEDFFETKNQ
                     NIYKALLEISSYFKSKGLKIGIDAFSPFISYFTGQDLEKLQEVADFIKPMMYRTTKAP
                     AGLPFEFERFIDESIHKNKEIGKRNFLDILGIKEYEGDVYPIEFVLEEIDFMTRNLNK
                     DIYVGVEINKKEIISPVTPKYISENISNINKTTVKGYVLSWDLLSAPKENIDEIIKWF
                     RN"
     gene            26677..28173
                     /locus_tag="PC414_000826"
     CDS             26677..28173
                     /locus_tag="PC414_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDF2463235.1"
                     /translation="MSKILEIQQIYKSFGKVDVLKGINFELEKGEVRALLGANGAGKS
                     TLIKILGGVHSQTKGKVILTGNEIMFKNPHHSKENGISIIYQELSLIPTLTVLENIFL
                     GREILKGSVFLNKKDMLEEYMKICEDFDFNIDPHIVVSNLSVANQQMVEIMKAVSCNS
                     EIIIMDEPTTSLTNNEKDGLFKIISRLKSKGKTIVYISHILEEVFKVCDSASIMKNGI
                     MIGTYDVEDLTKLKITQLMTGNDNHKLIEKKDYTFAKYEEPPVLEVKNISWSNKIKNV
                     SFKVYRGEVVGLAGLVGSRRTEIINLIFGIDNKDSGDIFINGKKVDITSPKVAIKNKI
                     GLIPEDRKNLGLILEQAIYTNSSSVQIDKFKKFLFIDRNKEIEFSKKGVKELGIKVSD
                     VKQEVKELSGGNQQKVVVSKWLEKDLDLIIYDEPTKGIDIAAKEDIFNTIKEFSSKGI
                     GVIFISSDLEEVIRVSDRILVTREGSIVDDLKNENIQVQDIMNSIFNV"
     gene            28196..29146
                     /locus_tag="PC414_000827"
     CDS             28196..29146
                     /locus_tag="PC414_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2463236.1"
                     /translation="MKKAFEKLNLRDYGVLLGFLALCIAISIATPSFLGKQNILNLLR
                     QSSIIGIISAGMTFVIISGNFDISVGAVAALAGAITMKFATGGTNLFASMFLGVAICA
                     VIGLINGIVVAKINVPSLIATMAMVTIVRGLLLMLTGGYPITENIPILDYIGNGYLFN
                     IPIPVVIFFIVVLASFIVLNKTKFGRYVYSVGGNQEASKLNGINVDSHKVKVFIINAV
                     LAGIAGIVLTGRLGTATAIAGEGYDMDAIASVVIGGTSVAGGSGSVLKTVIGVLLMSV
                     INNSFNLLGIDVYFQYIFKGLIILAAVGFDSYSKKKLASR"
     gene            29167..30129
                     /locus_tag="PC414_000828"
     CDS             29167..30129
                     /locus_tag="PC414_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDF2463237.1"
                     /translation="MKKLMKHLALLLSLVMIFGLVGCSNGGDKEKNGEKKIAVLLPGS
                     TGYFVATKQGIDAKSKELGVSVEYADAQWDASKQLSQAEDFMAKGVDMIALCGVDSAV
                     SERIVKAANDSDVPIVAFTNSIGSNPTGEFKGLVTYIGQNEEETGALTGKIAKNLLGE
                     TGGKAVLIEGVPGTTPQVLRKKGLEKELKDSNIEIVYNQTSNWEKEQALKVTEDLIQK
                     KTDFNVIICQDDNSATGAGQALKDAGLKDKVKVIGLGGSKDGLKAITDGLIDGTTYMS
                     AVEEGGLVIEKASKFLKGEKIEPVTQIKQVEVNKDNISEFKGEW"
     gene            30501..31133
                     /locus_tag="PC414_000829"
     CDS             30501..31133
                     /locus_tag="PC414_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF2463238.1"
                     /translation="MARRLFLERIGLIFLGSAILAFGVYNFYYLNNITEGGVLGILLL
                     LKNLFNIQPAIANVVIDGLLLLVGYKFFGKKFLIYSIVASITFSVLYDLFEAIGPLVP
                     QSQNMLLSTILAGVTVGTGVGIVVKAGCASGGDDALALVISKTTSLNIGQVYLATDVI
                     VLLLSLFYLSAFDIFYSLIAVTISGKVIDFIYYHGKSLDMDISNDIVPEC"
     gene            31544..31636
                     /gene="kdpF"
                     /locus_tag="PC414_000830"
     CDS             31544..31636
                     /gene="kdpF"
                     /locus_tag="PC414_000830"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HDF2463239.1"
                     /translation="MEGLPMWFLAVVIIMLIIYLVYALINPEKF"
     gene            31677..33359
                     /gene="kdpA"
                     /locus_tag="PC414_000831"
     CDS             31677..33359
                     /gene="kdpA"
                     /locus_tag="PC414_000831"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HDF2463240.1"
                     /translation="MFMVQIVIVLAIFMILVIPMGKYLYHIATNQKTFGDRLFDKVDN
                     FIYKVCSIDKKKEMNWKQYALALLFTNAVMVFIGYIILRTQSMHIFNPSGIKSMEQGL
                     SFNTIISFMTNTNLQHYSGESGLSYFSQMTVIIYMMFTSAATGYAAAMAFVRGLVGKK
                     KTLGNFYVDLIRITTRVLLPGALIIGLILVTQGVPQTFAGTETVTTIEGKLQDIARGP
                     VAALESIKHLGTNGGGFFGSNSSHPFENPTIISNIVEILSMMILPGACVVAFGHMIKN
                     KKQGWVVFGAMSIIFLIGLVVCFKAESAGNPILSQLGLNQSMGSMEGKEVRFGIAQSS
                     LFTTVTTSFTTGTVNNMHDTLTPLGGLVPLLNMMLNVVFGGKGVGLMNMLMYAIIAVF
                     LCGLMVGRTPEFLTKKIEGKEMKLIALLIILHPLLILMFSGLSVAIPAGLEGISNPGF
                     HGLSQVLYEFASSAANNGSGFEGLGDNTMFWNITTGVVMFFGRYVSIIVLLAISSLLA
                     SKKAVNESIGTLRTDNFTFTIVLVLVVLIVGALTFFPALALGPISEHLVLWH"
     gene            33384..35441
                     /gene="kdpB"
                     /locus_tag="PC414_000832"
     CDS             33384..35441
                     /gene="kdpB"
                     /locus_tag="PC414_000832"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HDF2463241.1"
                     /translation="METKKTKFITKDIFKTSIIEAFKKLNPKYMMKNPVMFVVEIGFI
                     VTLILTIVPSLFGDEGNNLRAYNGIVTIILFITVLFANFAEAVAEGRGKAQADTLKKT
                     RKDTVARVIDTLGNEKMISASELKLGDIVLVNAGEVIPNDGEVIEGIASVDESAITGE
                     SAPVMRESGGDFASVTGGTTVVSDWLKIRITTKPGESFLDKMIALVEGASRQKTPNEI
                     ALNTLLVGLTLIFMVVLISLYPMANYAGVKIPISTMIALLVCLIPTTIGGLLSAIGIA
                     GMDRVTRFNVIAMSGKAVEACGDVDTMILDKTGTITYGNRLASEFIAVGNADSKDLIN
                     YSVMCSLKDDTPEGKSIVDLGMKLGSTGKTKEAEEAEFVEFSAQTRMSGINLADGRAI
                     RKGAYDSIIKRVKESGGTVPEDLEENVNRVAKLGGTPLVVCVDNEIYGIIYLKDTVKS
                     GLVERFARLREIGIKTIMCTGDNPLTAATIAREAGVDGFIAECKPEDKIDAIKKEQLE
                     GKIVAMTGDGTNDAPALAQADVGLAMNSGTTSAKEAANMVDLDSDPTKILEVVEIGKQ
                     LLITRGALTTFSIANDVAKYFAIIPAIFTLAIPQMEILNVMKLSTSFSAILSALIFNA
                     IIIPCLIPLAMKGVKYRPMKSQAMLMRNMLIFGLGGVIVPFLGIKLIDLLITPLVMVL
                     NLG"
     gene            35460..36086
                     /locus_tag="PC414_000833"
     CDS             35460..36086
                     /locus_tag="PC414_000833"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HDF2463242.1"
                     /translation="MKTLKPALLVSIVLLVVCGLVYPLVLTGVSQVAFKDKANGSMIE
                     VNGVKVGSELIGQSFTDARFFKGRVSSVNYNTYTKEDLVPDKDGNTSYGGVSSGSFNY
                     GATNPELHDRVQKDIEKFLKDNPTVKREDIPTDLLTASGSGLDPNISPASAKIQIPAI
                     AKASGISESKLQKIVDDNTSKKLFGVLGEDRVNVLKVNVEVAKILGLI"
     gene            36323..37231
                     /gene="cysK"
                     /locus_tag="PC414_000834"
     CDS             36323..37231
                     /gene="cysK"
                     /locus_tag="PC414_000834"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HDF2463243.1"
                     /translation="MLYNNALELIGNTPVVRLNNLGCTNGCSNIYVKLEKVNPAGSIK
                     DRAVYEMLEGLEQRGELKKGDVLVEATSGNTGIALSMIGKLKGYEVVIVMPETMSVER
                     RDLMKAYGANLVLTDGKAGMAGSIEKANELLKENPNYKSLKQFENKDNPNAHYKTTAV
                     EIMNDVKDLDIFVCGVGTGGTLIGTARYLKEQNPNIRVIALEPKKSPAISENKTGPHK
                     IQGIGANFVPENYDSSVVDEVILVDDEDAFDTVKLLAAKEGILVGISSGANIFGAIEM
                     AKKYPDKKIVTVAPDGVDKYMSMGIF"
     gene            37258..37848
                     /gene="cysE"
                     /locus_tag="PC414_000835"
     CDS             37258..37848
                     /gene="cysE"
                     /locus_tag="PC414_000835"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HDF2463244.1"
                     /translation="MFKKINKDIEYIMKNDPAARSKIEVFLLYPSVHAMIMHRMAHAL
                     YKKKKLFTARLISQISRFMTGIEIHPGAKMGEGILIDHGMGVVIGETAEVGNRVTIYQ
                     GATLGATGKDTGKRHPTVGDDVLIGAGTKILGPLNIGSNSKIGANSVVVKDVPNGATV
                     VGIPAKIVKIRNLEPVKKNKKEVSYEYDELDNVYYI"
     gene            38008..38196
                     /locus_tag="PC414_000836"
     CDS             38008..38196
                     /locus_tag="PC414_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HDF2463245.1"
                     /translation="MKANVNQDTCIGCGLCPSICPEVFDMKDDGKSHVIVDEVPSDAE
                     ESAAEARESCPVDAIDVH"
     gene            complement(38250..39119)
                     /locus_tag="PC414_000837"
     CDS             complement(38250..39119)
                     /locus_tag="PC414_000837"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HDF2463246.1"
                     /translation="MKLNRNDSCWCGSTKKYKKCHMDMDNKIDSFAKLGHIVPTHDII
                     KNESQIEEIKKSAVINTKILDYISDKIEIGMSTEDINKLVHDFTLQNGAIPAPLDYNG
                     FPKSVCTSVNNEVCHGIPSKDIILKDGDIINVDVSTIFNGYYSDASRMFMIGNVNENA
                     KKLVQVTKECVQKGLEVAKPWGFLGDIGDAINSYAKKNGYSVVREIGGHGVGLSFHED
                     PFVSYVSKKGTEMLLVPGMIFTIEPMVNEGTHEIFVDEENNWTIYTKDGKLSAQWECM
                     VLVTETGVEILTE"
     gene            complement(39316..40560)
                     /locus_tag="PC414_000838"
     CDS             complement(39316..40560)
                     /locus_tag="PC414_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463247.1"
                     /translation="MENNLNTAKRNNTELLQNDSLKVNLQIGVDFTLLSKSLKAFYKK
                     EKDFYCIALAPCNVSSEKANRKISIYDMIKEINILIESITDKNELLSEELFTNNLSEF
                     YDESLDEIYIDLKQAYLYIKKNTDGLNIEDSIEYVFDVDISNDIKAKENPLINFNSLS
                     LVVWSGETKHILQQMNILDLEDIHSYSIENNTNITIYENNTLQYKFDEESENIKDSSI
                     DIIEQMLILPDDLNYDKDEVENMKNRLAKINIKYLQTLKEKDIKIKLINSNLTDEPEF
                     SDLKYQLPPCWVRSGKTWKDVPGIYRNNSIVAKIGYSNPSYANVHSSKNLELHETAHA
                     IDKNVLNKKSNSEEFMEVFAQERYKLYDPKQVAHAYISKFIEEFFAESFVHYYLDEDS
                     KNTLKENCPLTYDFLEKLELNY"
     gene            40851..41162
                     /locus_tag="PC414_000839"
     CDS             40851..41162
                     /locus_tag="PC414_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDF2463248.1"
                     /translation="MYFTYIIRCKDDSLYTGYTSNIVRRMNEHKLGINSKYTRAKGFE
                     KLEVYFVTNTKSNAMKLEYYIKKLTRNKKLSIIKNPSILINLIDNKEDYIIGKEIEQL
                     T"
     regulatory      41255..41347
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            41447..42241
                     /gene="thiD"
                     /locus_tag="PC414_000840"
     CDS             41447..42241
                     /gene="thiD"
                     /locus_tag="PC414_000840"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDF2463249.1"
                     /translation="MSNYKIPTLTIAGSDSSGGAGIQADLKTFSAIGTYGMSVITAIT
                     AQNTKGVFAVEDLNKKIIKKQIEAVFEDIPPRAVKIGMVSSPEIILEIVENLKKYNPK
                     YLVVDPVMISKSGYYLLKPEAKENLIKYLIPLAYIITPNIPEAEEITGIKIHNVDDMK
                     RVGEEILQLGPKFVLMKGGHLDGEAVDILVGKNIFKVYKSERIDKKNTHGTGCTLSSA
                     ITSYLALGYEITEAVNLSKIYITEAIKRSFDIGHGVGPVHHFYKFE"
     gene            42265..43053
                     /gene="thiM"
                     /locus_tag="PC414_000841"
     CDS             42265..43053
                     /gene="thiM"
                     /locus_tag="PC414_000841"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HDF2463250.1"
                     /translation="MYNLIKDVKKLNPLVIHYTNNVTINDCANVTLAVGASPLMSFSY
                     EEVEEMVSVANSVVINIGTMNSNMLDLFLLAGKAANKYNKPVVLDPVGVFASKARAEL
                     TSRLLNEVKFSVVKGNVSEIKFIGGFNVRGKGVDSFDEEEDSTEIIRKIAEKLECVVV
                     ATGKIDIITNGKGTYKINNGTDKLKGITGTGCMTASLIASFMAVTENILEAATMGVLT
                     MSLSGELANLNNPPIGTFKENLMNAIYQMDIDALSKNSNIEFLN"
     gene            43076..43708
                     /gene="thiE"
                     /locus_tag="PC414_000842"
     CDS             43076..43708
                     /gene="thiE"
                     /locus_tag="PC414_000842"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HDF2463251.1"
                     /translation="MIDKESLKKCLKLYLVTDSEMLKGRDFYKCLEDAISSGITTVQL
                     REKNASGREFLRKAMKLREITKRYGVKFIINDRVDIALICDADGVHVGQSDIDVREVR
                     KLIGNNKILGVSARTLEEAICAKNDGADYLGVGSIFTTSTKLDAKSASFETVKEIKEK
                     VDMPFVLIGGINLDNIDKLKCLESDGYAIISAILKAEDISKEVEKWTLKI"
     gene            43847..44149
                     /locus_tag="PC414_000843"
     CDS             43847..44149
                     /locus_tag="PC414_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2463252.1"
                     /translation="MIRILLVCVGGMSSTLLVNKMEKDAKKRNIDCKIWAVGEGDIKS
                     ELDNFDILLLGPQLRFMLDDVKSIVGDRAPVSIIDMVNYGTCNGHAVLNSVLEILK"
     gene            complement(44215..44970)
                     /locus_tag="PC414_000844"
     CDS             complement(44215..44970)
                     /locus_tag="PC414_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2463253.1"
                     /translation="MGMLNILWRSMKWRFKNPISFVVTILQPFLWLVLYSSIANQTMN
                     NININNYTAFILPGIIVLVVFSSCSSGGIINFIMKNSGSFYRVLIAPISRYSIVLGQL
                     LEAILVSFIEVTILCIVSIFFSVRIESGIGGILLMIVLIFMTAFFLSSLAYSISLLLP
                     NEIVYETIMTAIVLPIFFLSSALFPIESLSGGLKVAVMLNPFTHVINALRSLIFGETI
                     LIGDIMPVILLFLILCCSSFSLAMWRLKKEMVS"
     gene            complement(44958..45920)
                     /locus_tag="PC414_000845"
     CDS             complement(44958..45920)
                     /locus_tag="PC414_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2463254.1"
                     /translation="MLAIEIDNLVKEYKNGVKALNGLSFNVNAGEIFSLLGPNGAGKS
                     SLINILTTFYKPTSGNVTMFGKDLVDNPSWIRTQIACVAQQISIDEHLSLMENMVFQS
                     KMYKVEPQIAKQRIDSLIDKFDLSSYLKYPTSSYSGGVKRRLDIAMNMVSSPKILFLD
                     EPTVGMDVDSRKSMWDMLLKIRDEYGTTIFLTTHYLEEAEQLSDNICIMKNGKDLAQG
                     TPSSLRSYIRQNILRITFHNTEDIKKYKDSIKSTGLVKFMSVRENSIFISVNDSRTAF
                     TLINKWLLEHDIEFDAIEIVEPSLEDVFLALTSSKKSLKEEWEC"
     gene            complement(45924..46382)
                     /locus_tag="PC414_000846"
     CDS             complement(45924..46382)
                     /locus_tag="PC414_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HDF2463255.1"
                     /translation="MNCKIEIKNIESVTVATMRYNGPMTEATKYFPNVFKAIKGKSNG
                     APFFCYYDVDQKTGVAEMELCVPTAETPNRMGITTKEFPKTKALFFTHTGSYSNLPKT
                     YEMIFKYIHENNIKIQTPWREVFIKGPGMLIKGNPDNYITEIIFPLKEEE"
     gene            46738..47094
                     /locus_tag="PC414_000847"
     CDS             46738..47094
                     /locus_tag="PC414_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2463256.1"
                     /translation="MNFELDNTTPIYLQIVKYIKRQIVTGELKPGETIPSRREMALNL
                     KVNLNTVQRAYKEMGDMNIINTFKNYQSSVTVDENILKNLKLELINESLSVFIEDMKA
                     INVSKEEVLKIIEDKY"
     gene            47166..47882
                     /locus_tag="PC414_000848"
     CDS             47166..47882
                     /locus_tag="PC414_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2463257.1"
                     /translation="MIEIKNVSKTYKRMQGLKIRKIEALKNVSFNIEKGKITALLGIN
                     GVGKSTMLKAIAGLINIDSGEIRIDGEKINEKVYNKLAFVPDVQSHFSNTTIKETFEF
                     MEIFYSKWNKEKSKEMMDIFKLDEDEIIDNLSKGNIARVKLILGFCQDPEYILLDEPF
                     TGIDLFKREEFIGVIAQYMEENQAIIITTHEIVEIESLVDEVVILDEGQIITSFNAEE
                     LREREGKSILDKMREVYKNE"
     gene            47875..48699
                     /locus_tag="PC414_000849"
     CDS             47875..48699
                     /locus_tag="PC414_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2463258.1"
                     /translation="MNKLLYLINVDLRRSNKFYIAYISFFSLITLSLNLFEINKFKNS
                     KFIMNLAIEDFGGIFYGLGILSNLGFIQSILFFGILGLFIYYIFMWKREFFSNNQSIY
                     TLMMLPQNKFKICISKSIALITMIYGFLISQTATLFICKYIFNFIFRNMPIINMNFAK
                     DLYYLNLKSIPIDFISFMAIYVFLLLIAISIVNCCILFIFSYINKFYVILITLLIIAG
                     LYFSTIYINIITLFRKYYNLIGFQFNNLVAGLLSFLIIMFILNGISYLLIKKKVSL"
     gene            48711..49910
                     /locus_tag="PC414_000850"
     CDS             48711..49910
                     /locus_tag="PC414_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463259.1"
                     /translation="MKLKSKISIFIIFLTLAIYFSIGLLNNEDNLKIKTIKGDSNELD
                     HININFGYALNGYINSYYTINGSNKTKKTELIKLQKDSKNTNNLIDVSSGSDLIAHVG
                     FEMNEESKDINFVIDEKKEDLEKGVFPSEGSDKEIEYEKLDMNNFTRTRYKVDKSTDD
                     YYAKILASKRYKKYLYVSVLYATSDSSENNKEYSSTIDIIKINPENKKIKTIKSINLS
                     NYFSEKYQNIENIGVSYASKYEDKMYILVNVLNTNIGDNSNSKFHLLEYNLEENKESI
                     MELKTDSKMALAKAKLEANKLYMVMSNDYDNSSITELNLLTYNILDKSLDEDKYVLDN
                     REKRGIRSIEIDSNKIVAYTSRLNSILSWDDKFMLYIINKNSKEIVYEGELQFNSSNS
                     MLEFEIK"
     gene            49919..50731
                     /locus_tag="PC414_000851"
     CDS             49919..50731
                     /locus_tag="PC414_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463260.1"
                     /translation="MKDLIRLFEFELKRNIKNYIFIIICCCSFVILNIVKNLNDYNYI
                     IENAVKSEQLTKIGNTIETVNVTGFSSFRNIMGSTESWFMFGIIACICYAFFIWYRDF
                     NGRSKSIYTLIMLPKNRINIYISKLLNILFLVYSYTVVLTISLFIASKLLPRHMLGNV
                     TNYGFVQETIYELKMLLPYSFEILFVEYIFLLIGFVSVVFTSILINKSIYKVSTLISF
                     LFLVIEILVFIISIEFIIPYEYSDVFTVLYSSINIFVCSLISNKLLKQKIDF"
     gene            50742..>51338
                     /locus_tag="PC414_000852"
     CDS             50742..>51338
                     /locus_tag="PC414_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463261.1"
                     /translation="MKAKNIGKYTICFIIVCFLMITMTLILSYFNKDKLSVKTLKGNV
                     KDIGRVSVVYTPNISKFSYEEVIISKNGVRNNTNPKSRIRNDYLNIDKKYSDFVNDKG
                     KEFIYKDDLNIGEAYTSVKSDLVQGMNALNDCIKVSNKNLKSGVEENFDINLNKSDFK
                     ENSWKTLFNKIYKGNLYMVISETNISGSKVNNICILKID"
BASE COUNT        20050 a         5379 c         8904 g        17005 t
ORIGIN      
        1 gaatttaaat ctgttgaata atattaaaac aatgataatg agtatgaatt tctaataaaa
       61 ataaatatca taaaaataag aaaaagtatt gggaggaaat gaaaatggca agaatgtatt
      121 atgaaaaaga tgtggattta gaagttttaa aaaataaaaa ggttgcagtt ttaggttatg
      181 gaagtcaagg acatgcacat gcacaaaatc ttagagataa cggtgtacat gtagtgatag
      241 gtctttatga cggaagtaaa tcagcacaaa aagcaaaaga agatggattt gaagttaaga
      301 gtgtagcaga agctacaaaa gaaagtgatt tgacaatgat gttaatgcct gatgaaaaac
      361 aaaagaaagt ttatgaagag agtgtaaagg ataacttaaa agaaggccag acattagctt
      421 ttgctcatgg attcaatata cattataatc aagtacaacc accagaattt gttgatgttg
      481 taatggtagc tcctaaagga cctggtcact tagttagaaa tgtatttaca aagggaagtg
      541 gagttcctgc actatttgca gtatatcaag actacactaa aaaagctaca gagacagttt
      601 tagcatatgc aaaagggata ggagcaacaa gagcaggtgt tttagaaact acttttaaag
      661 aagaaacaga aacagattta tttggagaac aatcagtact ttgtggtgga ataagtgaac
      721 ttataaaatt gggatataaa acgcttgtag atgcaggtta tcaaaaagaa gtagcatatt
      781 ttgaatgttt acatgaaatg aaactaatag tcgaccttat atatgaaggt ggatttgaaa
      841 gaatgagata tagtataagt gacacagcag aatatggaga ttatgtatct ggtaaaaggg
      901 taataactga tgttgcaaaa caaggtatgc aaaatgtttt agaggatata caaaatggaa
      961 aatttgcaaa agcgtggatt aaagaaaatg aagaaggacg tgaaaacttc ctaaaaacta
     1021 gagaagaaga atataataca gaaattgccg aagttggtag aaatctaaga agtatgatgt
     1081 cattcttaaa ataaatatat attaaatgga ttagtttgag ggaaagagtg gtgtatagta
     1141 gtgcgtatga atggagcaaa ggtaatttta gaatgtttaa agaaagaggg aattgacaca
     1201 atatttggat atcctggtgg agccgtgata cctctttatg atgctctata cgattattca
     1261 gatgatttta aacatataag gacatcccat gaacaaggtt tagttcatgc tgcagatggg
     1321 tacgcaagaa gtactaatac agtaggagtt tgttttacaa catctggtcc aggagcaaca
     1381 aatgccataa ctggaatagc aacagctttt atggattctt cacctatggt tgtaatttca
     1441 gggcaggtgc caactagttt attaggaaaa gattcttttc aagaaattga cataactgga
     1501 gcaacattat ctatgacaaa acataattat ttagttagaa acactaaaga attagttcct
     1561 acaataaaag aagcttttag agttgcaaat tcaggtagaa aaggacctgt acttgtagat
     1621 gttccaaaag atttattttt agcagaaatg gattttagtg gtgaggacta tgatttatgt
     1681 caaatagatg attatatgga ttataagagt gattttgatc tagatgatga aactaatatt
     1741 aagcttttaa atgaagcaat agatatcata aaagaatcta aaaagcctat aatatatgca
     1801 ggtggaggtg ttaaatcttc agacagtgag gaaattcttg aaaaatttgc aactaagata
     1861 gatacacccg tactaaatac acttatgggg cttggaaata tagatagaaa gaatgaatta
     1921 tctcttggaa tggttggaat gcatggaagt agagaaagta atttagcact ttctaattca
     1981 gatttagtaa tagcgatagg tgcgagattt agtgatagag tgataagtaa aagttctgaa
     2041 tttgctaaaa atgcaaaaat aatacacata gacatagacc catctgaaat aagtaaaaat
     2101 atagaatcta atgtatcttt ggttggagat gtaaagctag tccttagttt attaattgaa
     2161 agggttgaaa gtaagaataa tagcaattgg aaagaagaga taaagagatt tagaaagagt
     2221 gagggtgtac aaacatatga atttcatcca caaaatatac ttaaaaaaat aaatgaaaaa
     2281 tatgaaacat taaagaaacc cactgtggta gtaactgatg tcggtcaaca tcaaatgtgg
     2341 gctgctaagt actggaattt taaaggtaat aaaagtttta taacatcagc agggttagga
     2401 actatgggat ttggtttagg tgcagctatt ggaactaaag ttggtaatgt agataaaaat
     2461 gtggttttag tcactggtga tggaagtttt agaatgaact gtaatgaact agctacagtt
     2521 gcaaattata atgtacctat gcttatttta ctactcaata atagaacatt aggaatggta
     2581 aggcaatggc aaaagttatt ttccaatcaa agatactctc aaactgacat taatgaaaat
     2641 gtggattatg ttaaactcgt aaatgcatat aacattgatg gatataaggt atccagtatg
     2701 gaagaattag gaaaggcgct agatatgata gattttaata aacctgtatt tttgcaatgt
     2761 gatatagata aggattatga tgtttatcct attgtagcac ctaatgatgc attggaaaat
     2821 ttgatatgta actaaaactt atactgaata ttgataaaat tttttattaa atttttatta
     2881 gtattcagtt tttgttgttt agagatagat tggcattttt tatttttgca aataaatcaa
     2941 tgactatata attaagaact tattaaattt taattataca atttagaata gtattacaac
     3001 ttaagtaaat aataattttg aataataatt tacggaggta ttcatatgtt taaacacgat
     3061 aaagcattat taaaagaagt aaaagtagaa agaccaaatc ctcaatatgc tgttttaatg
     3121 caagaacagt tgggtggtgc aaatggagag ttaaaagcag ctatgcaata cttgtcccaa
     3181 agttttagaa taaaagaccc tcaaataaaa gatttatttc ttgatatagc agcagaagag
     3241 cttagccata tggaaatggt agcacaaact ataaatttat taaatggaca tgatgttgat
     3301 tataatgcag ttaatacagg cgaaatagaa acacatgtat taactggtct atcaccagtc
     3361 ttaataaact catcaggagc tccttggact gctaattacg ttacagttac aggggattta
     3421 gtagctgact tactttcaaa tatagcttct gaacaaagag ctaaggtagt ttatgagtac
     3481 ttgtatagac aaatagatga taaatatgta aaagaaacaa tagatttctt acttaataga
     3541 gaagaagcac ataatgctct atttagagat gctttaaata aagttaaaga tacaggttca
     3601 aatagagatt ttggagttac agaagattct aaattatatt tcgatttgtc tactccaggg
     3661 ccaaatcacg atactaagat agatattaat cctccttctt ttgaaaaacc tttaaaaaaa
     3721 taatttgata ttttttattt tgttttagta gttataaaaa ttatttacta gaagaattaa
     3781 ttatatgaaa aattaaatat gtatagttaa aaatgggtat acacttatta taagtgtata
     3841 tctatttgtt tattgcaaaa catataataa aataacaaat gaggtggtat tttggacagt
     3901 aatagaaaac ctaataatct aataaatgaa aaatctccat atcttttaca gcatgcttat
     3961 aatcctataa actggtatag ttggaatgat gaggctttta aaaaagcaaa agaagaggat
     4021 aaaccaatat ttttaagtgt aggatattca acatgtcatt ggtgccatgt tatggaaaaa
     4081 gaatcttttg aagatgaaga agttgctgaa ataatgaata gaaactttgt ggcaataaaa
     4141 gtagataaag aggaaagacc tgatgtagat agtgtgtata tgacagtttg ccaagctatg
     4201 acaggtagtg gtggatggcc aatgaccata ataatgacac cagataaaaa accttttttt
     4261 gcgggaacat atttcccaaa atattcaagg tataatagac ctggagttat agatttatta
     4321 gagaatgtat ctgaaaagtg gaatacaagc agagatatat taataaaatc tggagatgaa
     4381 ataatagaag ctcttaagga tgattttggt gtaaaaaata ctgagggtga tttatcaaaa
     4441 gagatgttga gttcatcagt tagagtattt aaggctattt atgatgaaaa gtatggtggt
     4501 tttggtaatg ctcctaaatt tccaagtcct cagaatctta tgtttttaat gaaatattat
     4561 agtatagaaa aagacaaaga tgtgttaaaa atggtggaaa aaactttaga tggaatgtat
     4621 agaggaggct tgtttgatca tattggattt gggttttcaa gatactctac tgataaaaag
     4681 tggttggctc ctcattttga aaaaatgcta tatgataatg ctatgcttac tatagctttt
     4741 ttagatgcgt ataaaataac aaagaaagag ttgtataaag aaattgctat aaaaactata
     4801 gattatgtag ttagagagat gaaggataaa gaaggtgggt tttattcagc acaggatgct
     4861 gatagtgaag gtgaagaagg taaattttat acatttaatc ctcttgagat aattgaagtt
     4921 ttgggtgaag aagatggaat tttctttaat aattattttg atataactag tagtggaaat
     4981 tttgaaggca aaagtattcc taatcttata aaaaataaag aatatgagag acataatgaa
     5041 aagatagctg accttagtaa aaaggtattt gaatatagaa aagaaagaac atctttgcat
     5101 aaagatgaca agatacttac atcatggaat gcattgatga tagttgcttt aacaaaggca
     5161 tatagtactt taaaaaatga catatattta gagtattcaa ataagtgtct taactttata
     5221 aataataatc ttgtaaatga gtcaggtaga ttattagcta gatatagaga tggaagttcc
     5281 gactatttag catatttaga tgattatgct tttttaatct gggcgtatat agagctttat
     5341 gagtcaacat ttaatatgaa atatttagaa aaggctttaa atttgaatga aagctgtatc
     5401 aacctttttt gggattatga aaaaagtgga ttttatatat atggtaaaga tagtgaaaat
     5461 ttaatagcaa ggccaaaaga tttgtatgat ggagctattc catcaggaaa ttctgttcaa
     5521 ttatacaatc ttataagact tgcgaaaatt actggagata atagacttga agaaatgtca
     5581 tataaacaat taaaactgta tgtggataat gtaaagagtt ctccagctgg ctacagcttt
     5641 tatatgcttt cattgatgtt tgaactttat tctactaagg aaattatatg tatttttaag
     5701 gaagattctg atttaattgc ctttaaagaa ttgataagtg aaaattttat accaaatgca
     5761 acttttttag caaagaaata caatgaagaa aacactatta taggtttttt aaataactat
     5821 agattgaagg atgataagat tagttattac gtgtgtcaaa gtaatagttg tagtcaacca
     5881 attaatgatt tacaaaaatt aaaagatatg attttaggag tagaataatc ataatagtga
     5941 aaaaggagat gctttaaaat aaaagtctaa tcatctcctt tttttgtaca aaaaacagaa
     6001 aaagagttcc tgagaacacc taatctatgt aatgattgtt ttgttaaaaa ataaaaatga
     6061 cattatagtt atataaaata ttgtactata aaaaagtagt attatagatg cgatagtatt
     6121 aatcgttttt atttactctc tttcttcttt ttgcaaattc ttcaagtgct tcagatttaa
     6181 ttttatctat ttcttcatat tccataaaat tagcatcttc ttcaaagcca tcttgttctt
     6241 caccattaaa acgatacatt ttttttagta aactccataa ggtctctaat tcatctttgg
     6301 taaattcgtg aaatacatct gccataaagt ttattccagt cctactacat gttaccataa
     6361 ccttttttcc taagtctgta actttaacat taattgctct cttatcatga ggacttggaa
     6421 ttacatcaac atatccattt ttttcaagat tagcaactag tctatttata ttttgtttac
     6481 ttgtacccat ttttcttgca atattattta atgttgtttc ttcctctggt aaatgaagaa
     6541 ttgataaaat agtcatatac tgtcttgaag taagtatacc aaaatattta tctccttcta
     6601 tttgtatttt gtttgtaagg gtaaaaagag ttgcatatgt ttgattcatt aaaaagagtt
     6661 cttttagttc atttgaataa tccatgaaat atatctcctt tatttttaga tgttgttttg
     6721 tcgaaaacag aatatcatga attaattcta atgtcaatat ggtgtcaaaa tatattaaag
     6781 tattactttt ttatgtaact attaattaat taaattattg tataatttta tgtaaaaact
     6841 tctatgtacc aatgataact tctatgagga attaataatt cttaaatttt ttagaaagat
     6901 tttgacttga aaatacttgt aatataatat ataaattaat atactgaata aaatcaatct
     6961 ggagggataa ttatgaagtg tacaaacttt actttcaaag gagaggaagg tttagatata
     7021 tatacatata aatgggaaga tgaaaatata aaaaaaccaa aggcagttat ccaaattgca
     7081 catggaatgg ctgaaactgc acaaaggtat gagacttttg ccaaggtact tactaaaaat
     7141 ggttatatag tatatatcaa tgaccataga ggtcatggga aaactgcaaa gataatagaa
     7201 aatgttggac atttagctga aaaagaagga tttagatgtc ttgtagagga catgtatact
     7261 ttaacaaata ttataaagaa agaaaatgaa gatctgccaa tttatctatt tggacacagc
     7321 atgggttcat ttgcaagtca aagatatata atggattaca gtaataattt atctggtctt
     7381 atactatgtg gttcaaatgg aaagcaagga atcattctaa atcttgctca tctgataatc
     7441 aatcgtgaga ttaaaaagta tggaagacgt tctaaaagta ataaaataaa taatttgata
     7501 tttggcggcg aaataataag aagaaatgaa aagactaagt ttgactggtt aagtagagat
     7561 aaagaacaag ttgaaaaata tataaatgac ccattttgtg gagtagtgtg tagttgtggc
     7621 tttttttatg atttagttca agggttaaaa gaaattgagg ataaagaaaa cttaaaaaaa
     7681 gtgccacttg atatacctat ttacataata tctggagata aagaccccat aggaaaaaat
     7741 ggtaaaggag tcttaagact aagggataga tatataaaat taggagtaaa agatgtaact
     7801 tgcaaacttt acaaagatgg aaggcatgaa ttgttgaatg aaataaatag agaagaagtt
     7861 tttgaggata taatttgttg gttaaataat aaaatagaaa tattgtaata aaaaaatata
     7921 atgatatatt tactttaaat ttatttatat ataatatttg aaaagtggat tatgtatatg
     7981 caaagtagaa aagagagagc ccagaagtga ttttttgcta taatgccatt tattgaatag
     8041 gtgggtgtta ttataaataa attattaaca ctcagtatta ttatcattgt atatggaatt
     8101 tattttattt aaaattcctt gaaagtttaa atatagtaag ttgtagtaaa gagaaaatat
     8161 aaatatttaa atgctattta atgtgaatct attaattagg ttatacttaa acttaattaa
     8221 tagattttat atttcaagac aatgagatag ggttttagat taaaatataa ccaatggtta
     8281 aattacagac attttgttaa ctttccctcg atttactagt aaatattttt tttatatact
     8341 taaattacaa cagatgtact agggaggtat atatgcaaat taaaataggt aaagtcatac
     8401 agtgtcttag aaaagaacga aatttaactc aagaacaatt agcaaagttt ataggtgtgt
     8461 caacaccagc agtatctaag tgggagagtg gaaattctta cccagatata gaattattgc
     8521 ctttattagc agactttttt aatgtgtcta ttgataagct tttaaattac aagattgatt
     8581 taagtgaaga agaagtaatg aaaatttata aagaattaga atcaggcttt gctagaattg
     8641 aaattgattt atcaacagaa gaaccaaaag aagaatttag acaagattta gaatctgtaa
     8701 aaaagctttc caatatgtat atagagaagt atcctaaaag ctatttattg aagctaagga
     8761 tatgttcgct gtatcagatg tattcatata aatttggtaa gagtgaactt aatgacagag
     8821 taaaagaaac aacgaatatt cttgaagata tagtgagaaa tacagatgat atccaaataa
     8881 aagaaacagc tcttattata ttatcaaacg catattgtat gttggaggat tatgaaaagg
     8941 cagagttgta tttaaatatg atacataagt caatagggga tacaagtgtt aatttagcta
     9001 tgatatattt aaagcaaaat agacttgaag aagcagaaat attgcttcaa aataaattgt
     9061 ttagtaatgt atttaatata agcatggatt gtaagggaat aattaatgta tacaaaaatc
     9121 aatacaagga attaaagaaa aaattggaga atagaaattt taataaaaat gcaattgaga
     9181 gagaaatgga gtatataaaa aataagttgc tggggtatgc aaatctttct ttagaaataa
     9241 aaaaaatgct cagtgaagat aaaggtgcat tttttagtat gtatatggat tatatggaat
     9301 tatctctaat ttttttattt tttaacatga aagaagaagc aaaaaaagct ttatatagtc
     9361 taaaagaaat attagaaaaa tatcctatac atgaaagttt ggatgtaagt caaatgagat
     9421 tttttgataa ggtagagtct aaaaatttat atacttttaa tatatataca aacttgttaa
     9481 ttgtacttaa tgatgacagt tataatgagc ttagagaaga accaattttt aaggatgtaa
     9541 ttgaaaagat tttagatatg gaaaaaatgt tgaaaaataa agaataagta aaaatttaat
     9601 ataggactta aagattaatc aaattaaaag aaagtgtttc aaaataaaaa tttgaatcac
     9661 cttttttgtc ggtcttaaac agttatttag aaaaatagag gtttcaaaat agaaatctga
     9721 attatctgta ttattacaca aaaatagaaa cctaaattta taaaaataga ataaaatttg
     9781 ttatataaaa tgtaattttg tctataaaat ctcacctgtt ttcttaactt taagcgtgaa
     9841 aaagaggatg tatcatgaat tttttagttc atgatgcatc ctgctttgta tgaaatccaa
     9901 tatatcttta ttatttaaac aatggaaaag agtcttattt atattttgag acatctcttt
     9961 tatttaatta tgaagtttta tacagttcaa taaataggaa ttatatttag aaactcatga
    10021 cattatttct tagatagtga ttaaatttat catattccat tttagataac ttttctgggt
    10081 aagacattac aatcaggtta tgcccattaa attttttatg gtatggtgga tgaatgtgtt
    10141 cataattatt tgtcttatat tttactcttt catagaacgc cttgcgataa attgaaatct
    10201 catcaattgg aaggtcaatt tctaaaataa tagtttttcc tttcttattt aaaagctcta
    10261 acgctctttg cccatatcta ttattgcgaa tttctggaaa aatgcagaaa tgttctacat
    10321 agataaagtt atctgtttcc caacataaaa ttatgccgac aaactggttt ttatcataaa
    10381 ttaaattgaa ttgatattct ttatttttca aaatttctgc ttgcaaagaa gattttcttt
    10441 gtttatgaaa tgggaaacta attttatata attccatagc cttgtaatat aatttatttt
    10501 cactggttct taaccgttca aatttcactt tactcctcct aatcacgaaa attccaattt
    10561 atttatgagt tcattttacc atagtcagta tttgcataca atgtgtttta agttgagtgt
    10621 catttctttc ttttatttat gcttatatct catagtctaa gagataattg cttaagacat
    10681 aactaaaaac tattatatta agctttgata atctaaaaac agtatattta tggacttttc
    10741 taggtctaaa agtaatctta ataattttag aagttacaaa aaagttacat aaatttggaa
    10801 agctataata tacatatata atataaggtt aaaactgtaa tgaataatat taatttaaaa
    10861 tttgaacatg gaaggagatt attatggaga aaagagaaaa gaggataata atatcatctt
    10921 tattaagtgt atcaatttta atgggtttgg caagtatata ttctatatta aataaagaag
    10981 acataagttt aaccgttaaa ggtcaagagc aaaaagtatc ttcctttaag aaaacagttg
    11041 aggaactttt ggatgaacag ggtgtaaagt ataattctga agataagatt aatccaagtt
    11101 tagatacaga gttaaaagat gatatgaaaa taaaagttgt taaggtaact aaaagtaaaa
    11161 aagaagagat tgaaaaaatt ccatttgata caaagcatgt aaatgatagt aatttgttaa
    11221 aaggaaaatc taaagtttat caagaaggtc aagaaggaga aaaaaaacta gtctataatt
    11281 taacttacca tgatggcaag ttagtcaaaa aagtcttatc aaaagaagta atatctaagg
    11341 agccaactac aaaaattata aaatatggaa ctaaagaaaa agtactaata gcatcaagag
    11401 gagcaaatat aagaggaggc aaacatatga aagtggttgc cactgcatat gcaggagata
    11461 caataacatc tactggtaca actccaagat ggggtgttat tgcagttgac ccacgtgtaa
    11521 taccatatgg aacaaaagta tatataccta aactgggtat gacttttgta gcagaagatt
    11581 gtggaggtgc aattaaaggt aatagaatag acatttttat gaacagtgaa ggaaaagctt
    11641 caaattgggg aaggaaaagt atagatatat atctacatta aattagtgaa tagaataact
    11701 ttacttgaag gacaaaaaag tataaaatat gaattaacta aactgttaga ttattcttaa
    11761 aacataaaaa tatatctaag gggggataag atgaaaatag tctgcttagg agacagttta
    11821 acgtatggat ttggagtatc acgaagtaat tcatggacaa acattgtaaa taaagagact
    11881 cgattagaaa tagtaaataa gggtataaat ggtgatacaa caagtggtat gttagctagg
    11941 tttaatgaag atgttgtaaa aaactctcct gatatagttt ttataatggg tggcaccaat
    12001 gattttattg ctggtgcggg aaatgaaatt attaattcta atataatggc tatggtgcat
    12061 cagtcatatt caaaaaatat tatacctata ataggtatac cattaaaacc agatattcca
    12121 aatgttaggg aagattggag ttgttttact gattttaata tagtatctca aaacttagaa
    12181 tcatatagtt attggataaa aaaattttgt atgacattta atactaattt cgtagatttt
    12241 tattcagaat ataataaaaa tatggaacta gaaggatata aaaaattata ttttgatggg
    12301 ttacatccaa ccaaagaggg tcatagaata atggcagaca tttttattaa ttcaattaat
    12361 aagtatatag aagagttata gattacatta aaatgcttgt ttatgcgtca gttgcaataa
    12421 atacatacag tagagagggg tttattatgg attatactaa aacttatgaa gagtggataa
    12481 agggttcata ttttgatgaa gatacaaagc tagagttgga gaatataaaa aataatgaaa
    12541 aagaaataga ggatagattc tataaggatt tagaatttgg gactgcgggt cttagaggaa
    12601 taattgaagc aggaacgaat agaataaata agtacacagt tagaagagct acttttggat
    12661 tggcaaacta tatattagaa aacacaacaa aagaagaaac aagtagagga gtagtaatag
    12721 ctcatgataa tagacacaag tctagacaat tctgtataga atctgcaaat actttggcag
    12781 catgtggtat aaaagcatat atatttgata gtttgagaac tactccagaa ttatcttttg
    12841 ctgttagaag cttaaatgca atcgctggta tagtcataac agcaagccat aatcctccag
    12901 aatataatgg atataaggta tactgggaag atggagcaca agttatgcca gagattgcaa
    12961 atgcaataac agagaaagta aatagtatac atgattatag tacaatacca accttaacgg
    13021 aagctaataa aaatttggta gttttgttgg acgaatcaca agatactaaa tttatagaag
    13081 cagtaaaaag ccaaattata agaaaagatt tagttaaaaa tgtaggtaaa agctttaaga
    13141 tagtttatac acctctttgt ggaacaggga atgttccaat aagaagagca ttgaaagaag
    13201 ttggttttga aaatataatt gtagttccag aagaagaaaa tccagaccca aattttgcag
    13261 ggcttgatta tccaaatcca gaagagaaaa aagctttaaa tagaggtata ttactcgcaa
    13321 aagaaaaagg agcagacctt gtaatagcaa ctgacccaga ttgtgataga gtaggtgtag
    13381 cagtaaaaac tactacaggt gagtatgctt tacttacagg aaatcaaatt ggtggtatgc
    13441 tgacacatta tataatagaa ggattaaaag aaaataataa attaaaagaa aatcctacaa
    13501 taataaaaac catagttaca tcagaatttg gagcagatat agctaaagca aataatgttg
    13561 atgtattaaa tgttcttaca ggttttaaat ttattggtga aaaaataaaa ttatttgaac
    13621 aaaataaaaa tagaagttat gtatttggat atgaagaaag ttatggttac ttagttggga
    13681 ctcatgctag agataaagat ggtgtagttt cttcattgtt aatatctgaa atggcagcat
    13741 tttactattc aaaaggtatg agtttatatg atggcttaat agaactttat aaaaagtatg
    13801 gatttttcaa agaacagact atatctttga ctttaaaagg tatagaaggt gtagaaaaga
    13861 taaaagaaat aatatcttat tttagagaaa atcaaattga ttatataaat agtattaaag
    13921 tagttgataa aaaagattat aaaaatggta tagataatct tcctaaatct gatgttttaa
    13981 aatacttttt ggaagatgaa tcttgggtag ctataagacc atctggaaca gaaccaaaac
    14041 ttaaatttta catagctgta aaaggagcta gtgatattga agcagataaa aagattcaag
    14101 gtttgaaaaa gtatatagat gacatggtcg aaaaattaaa ataaaatatt attattttat
    14161 aagataattt tgtaataata tttatttagc tgaaaattat aatttacttt cattgagtag
    14221 aatatggaaa tgttagtaga attgttattt tcagctttta tttgtgttat ttttacttaa
    14281 ataattatat tatagaatga atctgagtat aattaatatt gtataatgaa attaatttga
    14341 tagaaaaggg gatatttatg tttggtatag ataagaaatt gattgaatat ataattgcaa
    14401 aatccaatta cagtgaatta agttcaaagt cagcagaagt atctttcttc ttaatgttgt
    14461 ccatatttcc ttttttaata tttacaataa gttctatagc ttatataccg attcttcatt
    14521 taaataagta tatagctttg tttagaaata tgatgccaga aggtgctttt gctgtgttat
    14581 cttcaatcat agtttctgca atagacaata gaaacctgaa atttttagca gtcagttttg
    14641 tacttactat gtggacattt tcaagagcag taaaagctct tattaaagga atgaatagag
    14701 cctataaagt taaagaaact aggtcttttt ttaaaatttt atctatatca tttttattta
    14761 caataatgct tttggtcctt atttttttat ctatgatatt tttagtttat ggggaaaaaa
    14821 taggttattt tatttttaat cttgtaggat tggatgagat atttataaaa atatgggata
    14881 ttctaagata cactgtaggt ataataacaa taatagtgat atttacactt ttatataaat
    14941 acactccaaa taaaaagtta actattaaag aatctgcgcc aggtgctatt tttgctactt
    15001 ttgcttggtt tttagtatca tttttatatt cttattatac aaattactat gctaactatg
    15061 aagtaatcta tggaagtata gctgaaataa ttgtacttat gacctggatg tattttagta
    15121 gttggtctat cgttattggc tatgaggtaa attcaagatt gtattttaga aagataagac
    15181 atgaaatgtt aaagtaaagt atttaaaata aaagagagta tttagtatta taaatgtaat
    15241 tgaattaata aagaaatatt gacatttaag ttaaaaagtc tagaattttt ctgggctttt
    15301 tttattttta gtataacttg acaaatttta tttaatggga tagttttaat ttataatcat
    15361 ctttaaaaag atgactattg ataagtaaga gataaaattg gtaagtatca aaaaatatac
    15421 taaataaaaa ttatagtcct aatgagttaa tttctgtatt ttcaaataat gcagtattta
    15481 gtcatgtgtt tttatatgcg cctgtatttc taattaccaa tatattagaa taagctactg
    15541 taattagatt tagaaaaaag cgacataaat aattataaac aaataataaa ttacataaaa
    15601 ttaaatttat tcacaagtat atcttataaa atatgttata attaatagaa aattgtcata
    15661 taattaatta agtagtatta ttataaataa cattgaaagg tagtggttag aatatggata
    15721 cccataataa atatgtaaat tttataaaaa atatacctgt acctttttta tattgtagaa
    15781 ttgtaaagag acaagaagat atagaatatc gagtagaata tataagtaag ggtatgggca
    15841 aagtattaca attagaagaa ggtatttgtg ataaaaatat actagatgta ttgcctgtat
    15901 ttaagtctaa aaaatatttc aaagagttgt tctcaaatga agtggattgt ataaaaaggt
    15961 acattccgac acttaaaaat tggataaata taaaaaaaca gataataggg gattcataca
    16021 taattttgta ttttggtaaa attgtatttg attatagaca gataatcgat tcctttgata
    16081 aaaaggaaaa agtagcttat attaaagacg aagaaggcac atatatagat tgcagtgaaa
    16141 atttaatacc aatattaaat aacaacatta aaacaacgaa agatatcttt ggtaaaaatg
    16201 atatagaagt atggggagag aatacaggaa aattatttag agatgattat agagaaggag
    16261 tatctagtaa aaaaagattt ttacaaaatc tttttgaata tgaagaaaca ttttttatgg
    16321 ttgaaaaata ttttttgtat gatgaagacg aacttttagg tactataggg atagtagaca
    16381 atataatata tagtggttac agtaatagaa attataactc aaaagattta atgaagatga
    16441 tagaacactc aattccagag aatatgtttt ataaagatgt gtatggaaat tatattggat
    16501 ttaattcagg ttttttgaat ttagcttgta tgaataaaga agaattatta gggaagaata
    16561 gttataaaat atcagaagaa gaagctttaa tagataagat atttgaaagt gataagggtg
    16621 tagttgaaaa taaaaaagta gttacatttg agttaaatat aagtatgaat gatgaaaaca
    16681 agtgtattga gattacaaaa aggccgtttt ttgatagcta tggaagtgtt attggaataa
    16741 ttggaacagc aagggatata agtagaagaa aaagattaga agaggaaatg gataaaacta
    16801 ggatggaatt ttttgcaaat ttatcccatg aacttagaac gcctataaac ttaatatcat
    16861 cttcattaca agtgatagaa aaaaaagaag cagatttaat agaatcaaat gatactttaa
    16921 aaagaaattt aggtataata aaacaaaatg gaaatagaat attaagatta gtaaataatg
    16981 ttatagattt tacaaaaatg caatcaggat atttagattt taaaccagaa gaatctgata
    17041 taattgcatt tatagaggag atatgtatgt ctgtagctga ttttgcaagc caaaataata
    17101 tacaacttac atttgataca gaaatagaag aattttcaat gttatttgat tcagaaaaat
    17161 tggaaagaat aatattaaat ttactttcta atggaattaa gtacaataag aaagatggaa
    17221 aaataaatat atttttatat gtgaaagata atgtatttaa tatgaaaata tctgatagtg
    17281 gaataggaat accaaaagaa aaaatagata aaatatttaa taggtttgaa cagattgaca
    17341 atgaactttc ttacagagtt aaaggaagtg gtattggact ttcattagtg aaatctttgg
    17401 ttgagttgca tgagggaagt atatctttaa aaagtcaact tggtattgga agtgaattta
    17461 tagtttcatt gcctgttaga agtaaaaata atattgaaaa atataatcat aaaagagaaa
    17521 tcagcaatga attaagtaaa aaattagaaa tagagttctc ggatttataa gtttaaatat
    17581 aaaaaataaa attttaaaaa agtattgaca attgatttag aagggtttaa aataaattac
    17641 aacataataa ataaaaaggc gatgaatagg aagagtaaat ctaattcgat taccagagag
    17701 aagagtccgt tggctggaag actttttaaa ttagagggat tgaaaactac ctaggagcta
    17761 ctttctgaag tcatctgcat tagtagattt tagtaagaat agtcgttagc tgcgttaaag
    17821 tgtaaagtga gttttcatga catatgaaaa ctaaattagg tggaaccgcg ggaataatac
    17881 tctcgtcctt atgttataag gatgagagtt ttttgttatt taaaaatata aacagtaatc
    17941 ataataatta tagaaaatat aaaagcaatg aataggaaga gtaaatttaa ttcgactatc
    18001 agagaggaaa gttcacaggc tgaaagactt tctaagtttg agaaattgaa aactacctag
    18061 gagctgtggt ctgaagttac tttaaaaagt tttagtaaga ctaaccgtta actgcgttaa
    18121 agtgtaaagt ggattttatc aataaatgat aaaatcaaat tgggtggaac cgcgagtata
    18181 gactcgtccc tttatatagg gatgagtcta tttttattgc aataaaagat tgaaaataaa
    18241 aaaaaatcgg aaaattataa atttaggagg aaaataaaat ggagaataaa gtgaaaattg
    18301 gagtactagg atatggagtt gtaggaagtg gacttataga tataatagat aataataaag
    18361 aaaaaagaaa tatagaaata gttggaattt tagttaataa tttggaaaaa cataaggata
    18421 aaaaatattc taatataata actaataata tagatgatat atttaataaa gatatagata
    18481 tacttgttga agtcatgggt ggtttagaac catcattatc ttatataaaa aaagcattaa
    18541 acaataaaat tcatgttgtt acagcaaata aagatttgtt agctgaatgt ggagatgagc
    18601 ttgcaaaatt agcaagtgaa aataaagtaa gtattaaatt tgaagcttca gttgcaggag
    18661 gtataccagt attaaaacca ataatagaat cactagaagg aaataatata gatagtataa
    18721 atgcaatttt aaatggaact actaatttta tattatctaa aatgtatgat gagaatctat
    18781 cttatgatat ggcattaaga caagcacaag aattaggttt tgctgaagca aatccagagt
    18841 cagatgtatt agggtatgat gctgctagaa aactatcaat attatcaact ctagcttatg
    18901 acaatagagt atattggaaa gatgtgtatt tagaaggaat tacagatata gatgaaaaag
    18961 atattgaata tgcaaaaaaa ttaaattgta aaataaaatt gattggtcaa agtaagtatg
    19021 aaaatgataa agtaagtgct tttgttagac ctgttttagt agaaaaagat aatatccttg
    19081 ctagaataga caatgagttc aatgctgtca tagtgaatgg agattcagta ggagaagttt
    19141 cttttgtagg taaaggagct ggaagtttag caacaggaag tgcggtttat tcagatgtta
    19201 tagatataat agataataga gtttcaagta tagattcttt tactaaagat aaaatacaag
    19261 ttaataaaat agttagagaa aagtgtggag cacttcttag atttaaaaaa tgtaataaag
    19321 atgaaattct aaatatagtt gagaattgtt tagtaaaatt cgatatttta aatgatgatg
    19381 atgaacttgc tattatggtt tatgctgatt ctgaatatga aataaacaac agtttatgtc
    19441 taataaaaga taaaggatac tgcgaaaaaa tgaataaaat gttgaaaata agttaaaaaa
    19501 gaaaaaatat aagtttatat gtaacatatt ttgacgattt taatactata tagtatgaga
    19561 tgaaacaaaa catctcataa aaaaattaaa attaaaggga gagatatgtt atatggaaaa
    19621 taaaaaatgt tattcagaag attggtatga aagaggagaa tctacagcta aatggttcca
    19681 aaatgataga gaagaatatg aaagagaagc atatgatgaa gatagagaaa gaagaggttc
    19741 aaactgtgga tgttcagatt caggagaaaa tagacctaga aactgtgaaa gatttagaag
    19801 agaagctgag ataagagaaa gagaagcaag agaagcattc tgtgaatctt cagagaaaaa
    19861 gaaagaggca ttagcatatg aatgtgaagc tagaaaatta tgggaagaag cagaaaaata
    19921 ctgggatgaa tattcaaaat acaactataa aggaatcgaa tatttagcag aagctgctag
    19981 attatttgat gaaggtatgg aatgtgaagc tagaagaaat ggaaataatg gaggaaacaa
    20041 taataattgt tgccataaat gccataaatg taattgtaac tgctgtagaa aataaaaaaa
    20101 ttaattgaca aatcaatagt taaaatatat catattatgt ataataaaga gaaaattcag
    20161 aaaaaaataa tgattagaag aaatataagt atatttatta gacaaatagt gctcagttag
    20221 tgaggtgagt acaatatgat taaactccca tcctctagct tttgatagag ggtgggagtt
    20281 tttatttatt tatatatctt aaatgaggat aatattgtaa aataaatgag tatgagattg
    20341 taaaattagg aggtaaaaat gaaaagtatt aaaacatcaa gtatgaaaga aaaggtagat
    20401 gaaaagatat taagagagca agtaagttct ataatagaag atataagaaa taataaggat
    20461 gttgcactta aaaaatataa tgagaagttt gatagaaata caagagatga atttagaatt
    20521 actaaggaag aaataaagga agcttataag catgtagatg acgaatttat aaataactta
    20581 aagattgctg ccaaaaatat aagagaattt gctaaagctc aaaaactaag ttttgaaaac
    20641 ccttttgaaa aagaaattta tccaggtgta atcttaggtc aaactaatat tccaatagag
    20701 tcatgtcttg catatgttcc aggaggtcag tatccattgt tttcaacagc tcttatgctt
    20761 ataatacctg caaaagtagc aggagtaaaa agaatagtgg catgttcacc aactatgaaa
    20821 aatactgaaa agataaatcc taagacattg gtagctatgg atatagcagg tgctgatgaa
    20881 atatatgcaa ctggaggagt tcaagctata gcagcattta catatggaac agaaaagatt
    20941 aatccagttg acataattgt aggaccagga aataagtttg taactgaagc taaaagacaa
    21001 tgttatggac aagtagggat tgattttgtc gctgggccaa gtgaagttct tataatagcg
    21061 gatgaaactt caaatccagt ttatattgca gctgatttat tagcacaatg tgagcatgat
    21121 ttaaatgcaa gaggaatatt attgacaaat agcttggaag tagcacaaaa agttgaaaaa
    21181 aatatagaaa gtatgctaaa agatttgcca acaaaggaca tagcatatag ttcatgggaa
    21241 aataatggag agataatatt ggttgatgat atggaagaag ccattaaaat aagtaatttt
    21301 tatgcaccag aacatttaga agttgcagta aatgagtata atgatatttg tgatagatta
    21361 actaactatg gttctttatt tattggaaat ctttctgctg aggtgtttgg tgactatgtt
    21421 tcaggtacca atcatacatt accaacactt aaagcatcaa gatatactgg tggtgtttgg
    21481 gttggaactt ttatcaaaac ttgtacaaag cagatattta atgaaaaagc tatacaatca
    21541 ttagcacctg tagctgaaaa gctggcaaaa gaagagggat tatatgcaca tgcaaaggct
    21601 gctgaggtta ggtttaaaaa gtagtaaaaa attttatttt ggcagtcgag acttagtatt
    21661 agactgagat aaggaaaacg ctgagaacct gaataaatac tatattttgt gggtctcggc
    21721 gcttctttta tatctgtata taatagaata atactatctg tgtgtttaaa tactaactta
    21781 atatttcttt tctaaataat gtttatgaaa tagttaatat ttttagttaa tacttgaaaa
    21841 aaacttttgc taactgtgca aattcatctg catttgtcaa tatagcaggg tgtcctccct
    21901 gactaaaaat gtaaatactt gcttttgaaa taaaagttgc catttgcttg tactcttctt
    21961 caagattatg acgacacatt tcgtcttcct tacttcctgt aaataaaatt ggcatttcta
    22021 atttacacaa ttctttatga aacaaagggc gtttctcgtt tgcacattgc aatagagctt
    22081 ttgtatcaag attaacaaca ttctcccaat cttttccatg acaccattca tagaattttc
    22141 gtgcttgaat atcttgcttt gatttttccc ttccagtgat tagattatct atgaagttat
    22201 cattaagagt acgtccatca aagctatctg ctataattgc ttcaactaaa tcgggacgtt
    22261 ctaatccagt attaacagct gcccatgctc ctccacttgt tccaattaag ccaactttac
    22321 cacaattcaa atgttcaatc agtgctattg tctgtaaggc ttcatcatgc catatgtcag
    22381 gagaaaattg agaaactcta tccgactgtc cattacctaa aaaatcaatt aagatacatc
    22441 taaaattttc tgcatataat ggcacaagtg ctttaaacat attagaagaa ccagtattac
    22501 catgcaggaa aattatcggt ttaccttgtc catattcttt ataaaaacaa gaattacctt
    22561 gatactgaaa atatgacata tattttcctc ctttttattt gttaagttaa ttataacaat
    22621 ataggtatga atgaaaaacc tcatgttctt tcttagaata aaaatgtgcc acgcataaaa
    22681 cgtgacacat ttctaattat aaattttgct gtttaagtat tctttgtatg cttttctctg
    22741 tcaaaaagta tttctctgat aactgcttga tactcattcc attttggaaa tttatacaga
    22801 tttgagaatt acgagaagct aagacttgtt tggtgtttgt gttttcaccc caatgctttt
    22861 tgtgctcttg ctttttggga atataaatca ctttaccatc tatatattct tgcacttgct
    22921 caatcagttc tttaggcagt aagtcaatcg aattttgata gctcattttg cacctcctaa
    22981 ataattattc tttaggatta gcaaaggcta ctgccactca caattaaaaa taaaattttt
    23041 tattatgtac aatagccttt gctatgcaca agttccaatt cccataaact aaatactcct
    23101 ttctggtttg aaaaattcat ctgaacattg atttcgcttt taagatagag ttcaatgaca
    23161 aatttacatt tttattatat atattcagat agataattgc aactgtcttt gtatgaggat
    23221 attttattat agtcaatact ataatatgta tttatatcat caaactaata tgaacagttg
    23281 aaaggaatat gaacaataaa acttagtcag gaagaataat tatgaagcag acaatagaga
    23341 aatttgattt taaagtttta ttgaaaattt attgacaaat ttaacttgta aatattatac
    23401 taaacttaat aaataaagtt tataaagtaa ggtgttgtct atgaaaaata tagttggaaa
    23461 accgcaaacg ataaaaaaag taaatgaaga tttaataaaa aacattataa aaaatgaagg
    23521 tcctataaca aaaccagaaa tttcaagcat tactgaattg agtttagcta cagttaataa
    23581 gattgtagag caattagctt tgaaaaatga agttaaagta agtggactaa gtgaatctac
    23641 tggaggaaga agagctcaat tatttgaaat aaatgcaaac ttagaacaca taatagcttt
    23701 atattattat agaaattgtt gtataggggt agttgccaat ttattaggtg aaataattta
    23761 tcaagaagaa tttaatataa gaatggatac gtatgaaaac gttaaccaag atactttttt
    23821 tgttatagat actctaataa aaaaggaaaa aggaaacaat ataagggcta ttggaattgg
    23881 tgtcccagga gttgtaaaaa aaggtgtagt taaaaatata ccaactataa aaagttggga
    23941 aggtataaat ttagaaaatt taataaagag taaatacaat actaaagtgt ttatagaaaa
    24001 tgatacaaat attactacta ttgggattta tcaaaaacag tataaagaca aatataaaaa
    24061 tatggctctt atgtatttag aagatggtat tggttcaggt atagttataa atggtgaatt
    24121 gtatttaggt tctacaaatt ttgctggaga attaagttat ctaaatatag aatttggtaa
    24181 gtataactct aacaacttaa atcttgaaga ttatattatg agtttaagaa aaagatattt
    24241 agaaagtaaa gataataaat ttaagaaaga aattttatct ataatatcaa actttataag
    24301 gactcttgta tgtgtcctaa atccacaagc tatgattata cagtgtagta tattaaccaa
    24361 gagtgacatg gagtttatag aaaaagatgt aagcaataat gttggagaag aaaattttcc
    24421 taaattaata aaagtagact cattgagaga aggaagtata aatggagtaa ttagtatgtg
    24481 tttaaaagaa actgactatc aatattcctt atctaataaa aaaggaggat attaatgtga
    24541 aatatttggt ttcaatagat ggagggggaa caaaaactaa attttgtgta agtgacttag
    24601 atggaaacat acttaaagaa cataccactg gttcaactaa ttataagtca gttggaatta
    24661 aaaaaacata tgaaaatatt aataatggat ttaaaaaaat attaaaagat ttatatatcg
    24721 attatgacga tattgagtac actgtatttg gtatatctgg ttgtgattct cctaatgatt
    24781 ataagattat aatggatgaa atattaaaaa ttgggattaa caaagaaaaa atatatctgg
    24841 caaatgatgc agttttagct ttttatgcac aagctgattc ccctggctta gtaattgtgg
    24901 ctgggacagg ttcaattata ctgggaatta aagaagatgg agaaatatat agagttggtg
    24961 gatgggggta taattttagt gatttgggtt ctggatatga tatagggaga aaattattaa
    25021 agaaagtatt gctttattgt gatgaatgtc atgagtatag tgatttattt agttgtgtct
    25081 tggacttctt tggagcaaat agttttgaac aacttgcata tatgattact gatataaatg
    25141 ataatgttga aatagcaaac ttagcttcat tagtgattga ttgtgccgaa caaggtgata
    25201 aattggctat tgaaatatta agagaatcat caactgaact atctaaatta gctcaagtaa
    25261 tattgagaaa aatatctaat ccaatgaaaa tggataaaag cgaaataaat atagttttgt
    25321 ctggtggtac attaaataaa actgtatatg ctcaaatgtt aatgaataat ttaaaaacag
    25381 aaaatacaga taaaaattta aaatttatat ctcaagaaaa tcagccagta tatgggggga
    25441 taagacttgc tatggcatta gctaatagga gggtcaagca tggatagaaa aaaggtaata
    25501 gtacaaattt ttactggagg ttttaaggct aaagaaataa agtttaatga tataaaatgt
    25561 aaactactag aattattgga ctgtattgat atagaaaagg ttatcatggg atggagtgta
    25621 gatgaagaat tatatgtaga aactaaaaaa ctattaaata aatataatgt tgaactatat
    25681 ctttggttgc catgtttttc tgaaatagga ttacttgaaa aaagtaattt actaattgat
    25741 tatatgggga aggaagtcaa gggctatgct ttacaagagg atgaaaattt tgagttttat
    25801 tgcccaaata ataaggaaaa tataaaaaat ataaagaaaa tatatagtac ttattttagt
    25861 gatattaaat ttgatggaat ttttttagat aaaataaggt atggttcttt ttcgaataaa
    25921 ctaaatggtg tttttaattg tttttgtaaa gattgtttaa atatatataa aaaaaataat
    25981 attgataaag agtcgttaat gagagaaatg aataatgtgt ctaagggagt tatagataat
    26041 gaagatactc catttggtat aacagaatac attaatggaa aatacaaatt taaaaataaa
    26101 atatgggaag atttttttga aacaaaaaat caaaatatat acaaggcatt attagagata
    26161 agtagttatt ttaaaagtaa gggattgaaa ataggtatag atgctttttc tccttttatt
    26221 tcttacttta caggtcaaga tttagaaaaa ttacaagaag tagcagattt tataaaacca
    26281 atgatgtata gaactacaaa agctccagct ggtttaccat ttgaatttga aaggtttatt
    26341 gatgaatcca tacataagaa taaagagatt ggaaaaagaa atttcttaga tattttagga
    26401 attaaggagt atgaaggaga tgtatatcct atagaatttg tactagagga aatcgatttt
    26461 atgacaagaa atcttaataa agatatatat gtaggagttg aaataaataa aaaggaaatt
    26521 atatctcctg ttacacctaa gtacataagt gaaaatattt caaatataaa taagacaaca
    26581 gtaaaaggat atgttttatc ttgggatttg ttgtcagcac caaaagaaaa tattgatgaa
    26641 attataaaat ggtttcggaa ttagtgaggt gataaagtga gtaagatatt ggaaatacaa
    26701 caaatatata aaagttttgg aaaggtagat gttttaaaag ggataaattt tgagttagaa
    26761 aagggagaag taagagctct tttgggagct aatggagcag gaaaatctac acttattaaa
    26821 attttaggag gagttcattc tcaaactaaa ggtaaagtaa ttttgactgg taatgaaata
    26881 atgtttaaaa atccacatca ttctaaagaa aatggtatta gtataattta tcaagaatta
    26941 agcttgatac caacgcttac agtacttgaa aatatttttc ttggtagaga aattttaaaa
    27001 ggaagtgtgt ttttaaacaa gaaagacatg cttgaggaat atatgaaaat ttgtgaggat
    27061 tttgatttta atatagaccc acatatagta gtttctaatt taagtgtagc aaaccaacaa
    27121 atggttgaga taatgaaagc tgtttcatgt aattctgaaa ttatcataat ggatgaacct
    27181 acaacatcac ttactaataa tgaaaaagat gggctattta aaataataag tagactaaag
    27241 tctaaaggta aaacaatagt ctatatttct cacatattag aggaagtttt taaagtatgt
    27301 gatagtgcaa gtataatgaa aaatggaatt atgattggaa cttatgatgt agaggattta
    27361 actaaattaa agataactca acttatgaca ggaaatgata atcacaaact tattgagaag
    27421 aaggattata catttgcaaa atatgaagag ccaccagtac tagaagtaaa gaatataagc
    27481 tggtctaaca aaataaaaaa tgtttcattt aaagtttata gaggcgaagt tgtaggtctt
    27541 gcaggtcttg taggttcaag aagaactgaa attataaatt tgatatttgg tatagataat
    27601 aaagatagtg gagatatttt tatcaatggt aaaaaagtag acataacttc tcctaaggtt
    27661 gctataaaaa acaaaatagg attgattcct gaagatagaa aaaatctagg attaatttta
    27721 gaacaagcta tatacacaaa ctcatcatca gtacaaattg ataaattcaa gaaatttttg
    27781 tttatagata gaaataaaga aattgagttt tctaaaaaag gtgttaagga attaggaata
    27841 aaagttagtg atgtaaagca agaagtaaaa gagcttagtg gtggaaatca acaaaaagta
    27901 gtagtttcta agtggttaga gaaagattta gatttaataa tatatgatga gccaacaaag
    27961 ggaatagata ttgctgcaaa agaagatatt ttcaatacaa tcaaagaatt ttcaagtaaa
    28021 ggaataggag taatatttat atcttctgat ttagaagaag taataagagt aagtgataga
    28081 attttagtaa ctagagaagg ttctattgta gatgatttaa aaaatgagaa tatacaggtt
    28141 caagatataa tgaacagtat atttaatgta taaaaaatga aaatggagga ctgagatgaa
    28201 gaaagctttt gagaagttaa atttaagaga ttatggagta ttattaggtt ttttagcttt
    28261 atgtatagct ataagtattg caactccatc atttttaggt aaacaaaata tacttaatct
    28321 tttacgacaa tcttctataa ttggaataat ttcagcaggt atgacttttg ttataatatc
    28381 tggaaacttt gatatatcag ttggagcagt agcagctctt gcaggtgcta taacaatgaa
    28441 atttgcgact ggtggaacta atttgtttgc atccatgttc ttaggtgtag cgatttgtgc
    28501 agtaatagga ttaatcaatg gtatagtagt tgcaaagata aatgtaccat cattaatagc
    28561 cactatggct atggtcacta ttgtaagagg attgctgttg atgcttacag gaggatatcc
    28621 aattactgaa aatataccaa tacttgatta cattggaaat ggatatttgt ttaatatacc
    28681 aataccagta gtaattttct ttatagtggt gttagcttcg tttattgtac ttaataaaac
    28741 taagtttggt agatatgttt attctgtagg agggaatcaa gaagcaagta aacttaatgg
    28801 tataaatgta gattctcaca aagtgaaagt gtttattatt aatgctgtac ttgcaggaat
    28861 agctggaatc gtactaacag gaagacttgg gactgcaact gcgattgcag gtgaaggata
    28921 tgatatggat gcaattgcat ctgtagtaat aggaggtaca tcagtggcag gaggttctgg
    28981 ttctgtactt aaaactgtta taggagtatt acttatgagt gttataaaca acagttttaa
    29041 tcttcttgga atagatgtat atttccagta catatttaaa ggattaataa ttttagcagc
    29101 agttggattt gattcttata gcaagaaaaa acttgcttca agataaaaaa taagggggat
    29161 ttttttatga aaaaattaat gaaacattta gcattgttgc tatcacttgt tatgatattt
    29221 ggtttagttg gttgttcaaa tggtggtgac aaagaaaaaa atggagaaaa gaaaatagca
    29281 gtattactac caggttcaac aggttacttt gtagcaacta aacaaggtat agatgcaaaa
    29341 tctaaagaat taggtgtaag cgttgaatat gcagatgctc aatgggatgc tagtaaacaa
    29401 ttatcacaag ctgaagattt tatggctaaa ggggtagaca tgattgcatt atgtggagtt
    29461 gattctgctg taagtgagag aatagttaaa gctgctaatg attcagacgt tcctatagta
    29521 gcatttacaa attctatagg aagtaaccca actggagaat ttaaaggttt agttacatat
    29581 ataggacaaa atgaggaaga aacaggagca ttaactggaa aaattgctaa aaacttatta
    29641 ggagaaactg gtgggaaagc tgtattaata gaaggagtac caggaactac tccacaagta
    29701 ttaagaaaaa aaggtcttga aaaagaatta aaagatagta atatagaaat agtttataat
    29761 caaacttcaa attgggagaa agagcaagct ttaaaagtaa cagaagattt aattcaaaag
    29821 aaaactgatt ttaatgttat aatatgtcaa gatgataatt ctgctacagg tgcaggtcaa
    29881 gctttaaaag atgctggtct taaggataaa gttaaagtca taggtttggg tggaagtaaa
    29941 gatggattaa aagctataac tgatgggtta atagatggta caacttatat gtctgctgta
    30001 gaagaaggtg gattagtcat agaaaaggca tctaaattcc ttaaaggtga aaaaatagaa
    30061 cctgttacac aaattaaaca agttgaagtt aacaaagata atatttcaga gttcaagggt
    30121 gaatggtaaa ttgtcataga taataaataa gatttttaga gttactttag aaactatata
    30181 aagtaactct ttttttaatt taatttagtg ataaattatt gaatagtatt tctaaacaaa
    30241 taaaaaatta atataaacaa agacttaatt atactaaatt ataagtaaaa ctaatataaa
    30301 cttatggaaa aaaggaaaac gtctaaagta ttggaattac tatagtgtag gtgtagtatt
    30361 aaaaaaacat aaaaaaacat aaaaaaattg ttgaaaaaaa tatagaaatt ttgtataatt
    30421 aaagatttga aaagtgcggt atacaaagcc aattcttttg tatataatat atataaattc
    30481 agaaaaagga agggatatgt atggccaggc ggttattttt agaaaggatt ggacttatat
    30541 ttttaggttc agcaatatta gcatttggag tttataattt ttattattta aacaacataa
    30601 cagaaggtgg agtgttaggt attttactac tgttaaaaaa tttattcaat atacaaccag
    30661 caatagcaaa tgttgtaata gatggtttac tacttttagt aggatataaa tttttcggta
    30721 aaaaattcct tatatattca atagtagctt ctataacttt ttcagtgtta tatgacttat
    30781 ttgaagctat aggacctcta gtaccacaat cacaaaatat gcttttatca actattttgg
    30841 caggtgtaac tgttggaact ggtgtaggta tagtagtaaa agcaggttgt gcatcaggag
    30901 gagacgatgc tttagcatta gtaatttcta agactacatc tttgaacata gggcaagttt
    30961 atttagcaac agatgttatt gtattgttgt tatcattatt ttatctatca gcgtttgata
    31021 tcttttattc tttgattgct gttacaatta gtggcaaggt aattgatttt atttattatc
    31081 atggtaaaag tttggatatg gatataagta atgatatagt tccagaatgt taatataaat
    31141 taaataactt tagattttat taggggtggc agatttgcca ccttttttgt gtctaaatct
    31201 ttatagaaag tataatccca tctaggtttt tgcaaatctc aaatatagaa ttatgttgga
    31261 atgactggga atacgattgc tagatgtgca ttttaaaaac ccaattttat atttatgaaa
    31321 tactaatcat taatggatgt ttcttaatcc tatctttata cctaaaatga taagataaat
    31381 gaagtagcaa tacaaaacta aaatttgtaa aagagattaa attaaatgga ggagctaaaa
    31441 tgttggatat agttatgata gcattgttat tcataggatt tgtttccatg aagttacttg
    31501 ctgattggtg tgggaaacaa actgaaaaaa aataatgtaa tgtatggagg gattacctat
    31561 gtggtttctg gcagttgtaa ttataatgtt aataatatat ttggtatatg cgcttattaa
    31621 tcctgaaaaa ttttaaacag tataaggcta ataagtaagt tgaattgggg ggaacaattt
    31681 ttatggtgca gatagtaata gtgttagcga tttttatgat tttagtaata cctatgggca
    31741 agtatttata tcatatagct actaatcaaa aaacatttgg agatagacta tttgataaag
    31801 tggacaattt tatatataag gtatgtagta tagataagaa aaaagaaatg aactggaaac
    31861 agtatgcatt agctttatta tttacaaatg cagttatggt atttatagga tatatcatac
    31921 ttagaactca aagtatgcat atttttaatc caagtggaat taaaagtatg gaacaaggac
    31981 tatcatttaa tacaataata agttttatga caaatacaaa tcttcaacat tattcaggtg
    32041 aatcaggact ttcatacttt agtcagatga cagtgataat atatatgatg tttacatcag
    32101 ctgcaacagg atatgcggct gctatggcat ttgtaagagg tctagtcggt aaaaagaaaa
    32161 cattgggaaa tttttatgta gatttaatta ggataacaac aagagtactt ttaccaggag
    32221 ctttaattat tgggcttata ttagttacac aaggagtacc acaaactttt gctgggacag
    32281 aaacagtgac tactatagaa ggaaagcttc aagatatagc tagaggtcct gtggcagctc
    32341 ttgaatcaat aaaacattta ggaacaaatg gtggtggatt ctttggttca aattcatcac
    32401 acccatttga aaatccaaca ataatatcaa atatagtaga aatattatct atgatgatat
    32461 taccaggagc ttgtgttgtt gcatttggtc acatgataaa gaataaaaaa caaggttggg
    32521 ttgtgtttgg cgcaatgtca atcatatttt taataggtct agtagtgtgc tttaaagcag
    32581 aaagtgcagg aaatcctatt ttatcacaat tagggcttaa tcaaagtatg ggaagtatgg
    32641 aaggtaagga agttagattt ggtatagcac aatcatcatt attcacaaca gtgactactt
    32701 cctttacgac aggtacagtt aataatatgc atgatacact gactccttta ggaggattag
    32761 taccactttt aaatatgatg ctaaatgtag tatttggtgg taaaggcgta ggtctgatga
    32821 atatgttgat gtacgctata atagcagtat tcttatgtgg attaatggta ggaagaacac
    32881 cagagttttt aactaagaaa atcgaaggaa aagaaatgaa gcttattgct ttacttatta
    32941 tattacatcc acttttaata ctaatgttct ctggtctttc agtagcaata ccagcagggc
    33001 ttgaaggaat ttcaaatcct ggattccatg gattatcaca ggtattgtat gagtttgcat
    33061 cttcagcagc caataatggt tcaggatttg aaggtctagg agataacact atgttttgga
    33121 atataacaac aggtgtagtt atgttctttg gaagatatgt gtctattata gtactactag
    33181 caatatcaag tttgttagca tctaaaaaag ctgtaaatga aagtatagga acacttagaa
    33241 cggataattt tacatttaca atagttttag tacttgttgt tttaattgtt ggtgctttaa
    33301 catttttccc agccttagcg cttggaccta tttcggagca tctagtgttg tggcactaaa
    33361 aaattgggag ggagtaaagt tttatggaaa ctaaaaaaac aaagtttatt acaaaagata
    33421 ttttcaaaac ttccattata gaagctttta aaaaattaaa tcctaaatac atgatgaaaa
    33481 atccagttat gtttgttgta gaaattggat ttatagtaac attgatactt acaatagtac
    33541 ctagtttgtt tggtgatgaa ggcaataact taagagctta taatgggatt gtaacaataa
    33601 tcttatttat aacagtcctt tttgcaaatt ttgctgaagc tgtagctgaa ggaagaggta
    33661 aagctcaagc agatacactt aagaaaacta gaaaagatac agtagcaagg gttatagata
    33721 cgttaggaaa tgaaaaaatg ataagtgcaa gtgagctaaa acttggtgat attgttttag
    33781 taaatgctgg agaagttatt ccaaatgatg gagaagttat agagggtata gcttctgtgg
    33841 atgagtcagc aataactgga gaatctgcac ctgtcatgag agaatcaggt ggagatttcg
    33901 catctgttac aggtggtact acagttgtaa gtgactggtt aaagataaga ataacaacaa
    33961 aaccaggaga atcgttctta gataaaatga tagctcttgt agaaggtgct tcaagacaaa
    34021 aaacgccaaa tgagattgct ttaaatactt tattagtagg gttaacactt atatttatgg
    34081 ttgttttaat atctctttat ccaatggcaa actatgcagg tgtaaagatt ccaatttcta
    34141 ctatgatagc tcttttggta tgtttaatac caactactat aggtggattg ttgtcagcta
    34201 ttggtattgc aggtatggat agagtaacta gatttaatgt aattgcaatg tcaggaaaag
    34261 cagtagaagc ttgtggagac gttgatacta tgattttgga taagacaggt acgattactt
    34321 atgggaatag acttgcatca gaatttatag cagttggaaa tgcagattca aaagatttaa
    34381 taaattattc tgttatgtgt tcattaaaag atgatacacc agaaggaaaa tctatagttg
    34441 atttaggtat gaaattaggt tcaacaggta agacaaaaga ggcagaggaa gctgagtttg
    34501 tagaattttc tgcacaaact agaatgagtg gtattaactt agctgatggt agagctataa
    34561 gaaaaggtgc ttatgatagc ataataaaaa gagtaaaaga atctggtggt acagtgccag
    34621 aagatttaga agaaaatgta aatcgagtag ctaaactagg aggaacacct ttagtagtgt
    34681 gtgtagataa tgagatatat ggaattatct acttaaaaga tacagtaaaa tcagggcttg
    34741 tagaaagatt tgctagactt cgagagatag gaataaagac aataatgtgt acaggggata
    34801 atcccttgac agcagctaca attgcaagag aagctggagt agatggattt attgcagaat
    34861 gtaaaccaga agataaaata gatgcaataa aaaaagaaca attagagggt aaaatagttg
    34921 caatgacagg tgatggaaca aatgatgctc ctgcacttgc tcaggcagat gtaggtcttg
    34981 caatgaatag tggtacaaca tctgcaaaag aagcagcaaa tatggtagat ttagattcag
    35041 acccaactaa gatattggaa gttgttgaaa taggaaaaca gcttttaata actcgtgggg
    35101 ctttgacaac atttagtata gcaaatgatg ttgcaaaata ttttgcgatt ataccagcta
    35161 tatttactct tgcaatacct caaatggaga tattaaatgt aatgaaactt tctacgtcat
    35221 ttagtgcgat attatcagct ttaatattta atgctataat tatcccatgt ttaatacctc
    35281 ttgcaatgaa gggagtaaaa tatagaccta tgaaatcaca agctatgtta atgagaaata
    35341 tgcttatatt tggtcttggt ggagttatag taccattttt aggaataaaa ttaattgatt
    35401 tattgattac acctttagta atggttctta atttaggtta ataaatggag gatataaaaa
    35461 tgaaaacatt aaagccagca ttacttgtaa gtattgtatt gttagttgtt tgtggacttg
    35521 tatatccact tgttttgaca ggtgtaagtc aagttgcatt taaagataaa gccaatggaa
    35581 gtatgataga agtaaatggt gtaaaagtag gttcagaact tattggtcaa agctttacag
    35641 atgcgagatt ctttaaggga agagtatcat cagtaaacta taatacttat acaaaagaag
    35701 atttagtacc agataaagat ggaaatacat cttatggagg tgtatcatca gggtctttta
    35761 attatggagc tacaaatcca gaacttcatg atagagtaca aaaagatata gaaaagtttt
    35821 taaaggataa tccaactgtc aaaagagagg atataccaac agatttactt acagcatcag
    35881 gttcaggact tgaccctaat ataagtccag cctctgcaaa aattcagata ccagctatag
    35941 caaaagcgtc tggaatatca gaaagtaaat tacaaaaaat tgtagatgat aatacttcca
    36001 aaaaactttt tggagtttta ggtgaagata gagttaatgt attaaaagtt aatgtagagg
    36061 tcgcaaaaat attaggtctt atatagtatt ttgtggaatt ttagcaacaa aatattacgc
    36121 aagatttaat ttaaaggtta aattttaaag agtgaaaatc gataatgatt ttcacttttt
    36181 tatttgaata ttactaatat ttggaataaa gtgattgtaa gtataaaatc caaaaaaagg
    36241 ttgcattatc aaaatgataa tactataata tatttatcgt tatcaatttg ataatagatt
    36301 tggacatata ggaggaaata gtatgttata taataacgca ttagagttga taggaaacac
    36361 acctgtagta aggctgaata atttaggctg tactaatggg tgttctaaca tatatgtaaa
    36421 attagaaaaa gtaaatccag caggaagtat aaaagataga gctgtttatg aaatgttaga
    36481 gggcttagag caaagaggag aattaaaaaa gggagatgta ctagttgaag caactagtgg
    36541 aaatactggt attgcattat ctatgatagg aaaattaaaa ggatatgaag tagtaatcgt
    36601 aatgcccgaa acaatgagcg ttgagagaag agatttaatg aaggcttatg gagcaaattt
    36661 ggtgcttaca gatggtaaag ctggaatggc aggttccata gaaaaagcaa atgaactttt
    36721 aaaagaaaat ccaaactata aaagcctaaa acaatttgaa aataaagata atccaaatgc
    36781 acattataaa acaacagcag ttgaaattat gaatgatgtc aaagatttag atatattcgt
    36841 atgtggagtt ggaacaggtg gtactttaat aggaactgca agatatttaa aagaacaaaa
    36901 tccaaatata agagtaattg cacttgaacc aaaaaaatct ccagctatat cagaaaacaa
    36961 gactggacct cataaaatac aaggaatagg tgctaacttt gttcctgaaa attatgattc
    37021 tagtgtagtt gatgaagtaa tattagtaga tgatgaagat gcatttgata cagttaaatt
    37081 acttgcagca aaagagggaa ttttagtagg gatatcttct ggagcaaata tatttggtgc
    37141 tatagagatg gcaaaaaagt atccagataa aaagatagtt acagtagctc cagatggagt
    37201 tgataagtat atgtctatgg gaatatttta ataattttat ttgagggagt gaatactttg
    37261 tttaagaaaa taaataaaga tattgaatat ataatgaaga atgatccagc agctaggtct
    37321 aagatagaag tttttttact ttatccatca gttcatgcaa tgataatgca taggatggct
    37381 catgctcttt ataagaagaa gaagcttttt acagcaagat taatatccca gatatccaga
    37441 tttatgactg gcatagaaat acatcctggt gctaaaatgg gggaaggtat attgatagac
    37501 catggaatgg gtgttgtaat tggagagaca gctgaagtag ggaatagggt gacaatatat
    37561 caaggagcta ctttaggagc tacaggaaaa gatactggaa agagacatcc aactgttggt
    37621 gatgatgtat taattggtgc tggaacaaaa atactaggac ctttaaatat aggttcaaat
    37681 tcaaaaattg gtgcaaattc tgttgtcgtt aaggatgtac ctaatggtgc aacagttgtt
    37741 ggtataccag caaaaatagt aaaaataaga aacctagaac ctgtaaaaaa gaataaaaaa
    37801 gaagtgtcct atgagtatga tgaattagat aatgtatatt atatatagga tttatataac
    37861 tataaaataa actttataaa aattatttta aaaggtattt ttttcaaatt tgaggacata
    37921 ataaaaaaga actgaatgtt acagttttgg ttcatcaatc tttgataaac aagattttaa
    37981 atttgtagcg tctaggagga ttttattatg aaagcaaatg ttaatcaaga tacttgtata
    38041 ggttgtggat tatgtccatc aatatgtcca gaagtatttg atatgaagga tgatgggaaa
    38101 tcacatgtta tagttgatga agtaccaagt gatgctgaag aatcagcagc agaagctaga
    38161 gaaagctgtc cagttgatgc aatagatgtg cattaataaa ataaatgcct ataaaaattg
    38221 aattttcaat ttttataggc atttgtcttt tattcagtta gtatttcaac acctgtttct
    38281 gtaactaaaa ccatacattc ccattgagca gataatttac catcttttgt ataaatagtc
    38341 caattatttt cttcatcaac aaaaatttcg tgagttcctt catttaccat aggttctatt
    38401 gtgaatatca tcccaggaac caatagcatc tcagtccctt ttttagatac ataacttaca
    38461 aaagggtctt catgaaagct taatccaact ccatgaccac ctatttctct aacaacagag
    38521 tatccatttt tctttgcata agagttgata gcatcaccta tatctcctaa aaatccccat
    38581 ggtttggcaa cttcaagacc tttttggaca cattcctttg taacttgaac taattttttt
    38641 gcattttcat taacattacc tatcataaac attcttgaag catctgaata gtatccatta
    38701 aaaatagtag aaacatctac atttataata tctccatctt ttaagataat gtctttactt
    38761 ggaataccat gacaaacttc attatttact gatgtacata cacttttagg aaatccatta
    38821 tagtctagtg gagcagggat agctccattt tgtagtgtaa agtcatgaac caacttattt
    38881 atatcttctg tagacattcc aatttcgatt ttatctgaaa tataatccaa aatttttgtg
    38941 tttattacag cacttttttt tatttcctca atttgacttt cattttttat aatatcatga
    39001 gttggtacaa tatgacctaa cttagcaaat gaatctattt tattatccat atccatatga
    39061 cattttttat attttttagt acttccacac caacagctat catttctatt taacttcata
    39121 acttttcatc cttcttttat tatttattta aattatgggt tacataaact attttaaaac
    39181 ataaccatta taaatattat aacataaata ctaagaaatc ctttatagat agtgatttat
    39241 tcaaagtata aattattata tggttattgt tgatgaaaag aggatagtta aatgtattat
    39301 tggatattag cgcacttaat agtttagttc aagtttttca agaaaatcat atgttagagg
    39361 acaattttcc tttaaagtat tttttgaatc ttcatcaaga taataatgta caaatgattc
    39421 tgcaaaaaat tcttctataa acttgcttat atatgcatgt gcaacttgct tagggtcata
    39481 aagcttatat ctttcctgag caaatacttc cataaattct tcagaatttg attttttatt
    39541 taaaacattt ttatctatag catgtgcagt ttcatgtaat tctaaatttt ttgatgaatg
    39601 tacatttgca tagctagggt tactataacc tattttagcc actatagaat tatttctata
    39661 tataccaggt acatctttcc aagtcttgcc tgatcttacc caacatggag gtaattgata
    39721 ttttaaatca ctaaattctg gttcatcagt tagatttgaa tttattaatt ttatttttat
    39781 atccttttct ttaagtgtct gtaaatattt aatatttatt tttgcaagtc tgtttttcat
    39841 attttcaact tcatctttat cataattcaa gtcatcagga agtataagca tttgttcaat
    39901 tatatctata ctactgtctt ttatattttc agattcttca tcaaacttat attgaagagt
    39961 attattttca tagatagtta tatttgtatt attttctata gaataagagt gaatatcctc
    40021 taaatctaaa atattcattt gttgtaaaat atgttttgtt tcgccactcc atactactaa
    40081 agaaagtgaa ttaaaattaa ttagtggatt ttcttttgct tttatatcat tgcttatatc
    40141 tacatcaaat acatattcaa ttgagtcttc tatgttaaga ccatctgtat tctttttaat
    40201 atataaatac gcttgtttta agtctatata aatctcatct aaagattcgt cataaaattc
    40261 tgataaatta ttagtaaaga gttcttcact taaaagttca tttttatcag ttatactttc
    40321 aattaaaata ttaatttctt ttatcatatc gtaaatactt atttttctat ttgctttttc
    40381 actagataca ttgcatggcg caagagcaat gcaataaaaa tctttttctt ttttataaaa
    40441 ggctttaagt gattttgata aaagtgtaaa gtctacacct atctgtaagt tgaccttaag
    40501 tgagtcattt tgtaataatt cagtattatt tcttttagct gtatttaaat tgttttccat
    40561 aatcattgcc ccctttttat tagggtatca taattttttt atatgtgtca atttgttttt
    40621 ttgaaaatta taattttttt gaaaatttta ttttttataa tttgtgaatt ttttattata
    40681 gaaataaata tgttattatt atgtcaaaat ttagcattag attcatgtag agtatgtttt
    40741 atacatatat tataattaat tctttgaaga ttgcatattc aaaatacaca ccattttttt
    40801 taattctata atataatatt tatatattta atgttaggat gtgatttaag gtgtatttta
    40861 catatattat aagatgtaaa gatgattctc tatacactgg atataccagt aatatagtta
    40921 gaagaatgaa tgaacataaa ttaggaatta attcaaaata cacaagagca aagggatttg
    40981 aaaaactaga agtttatttt gtgaccaaca caaaaagcaa tgctatgaag ttagaatact
    41041 atataaaaaa attaactaga aataaaaaat tgtctataat aaaaaaccca agtatactta
    41101 taaatttaat tgataataaa gaagattata ttattggtaa ggaaatagaa caattaacat
    41161 aaatatttat agtaacatta taatgggtgc atatggtgta aaaacttgac aataaatact
    41221 attgtaatat aatgaagtta taaatatagt aaattaaaat agctagggga gccaagagct
    41281 gagaggtata ccgaccctta cacctgatct ggataatacc agcgtaggaa agcttagtaa
    41341 aaattcaagt atgtattatt tgtgatatat gctatttcta agctatacct attgtaggta
    41401 tagctttttt aattaagtta tttaaattaa actttaggag gcaacaatga gtaattataa
    41461 aataccaaca cttacaatag ccggctctga ttcatctgga ggagctggga ttcaggctga
    41521 tttgaaaacg tttagtgcaa ttggtactta tggtatgagt gtaataacag ctataacagc
    41581 acagaatact aaaggagttt ttgctgtaga ggatttaaat aagaaaataa taaaaaaaca
    41641 aatagaggca gtctttgaag atatacctcc aagagctgta aaaataggaa tggtttcaag
    41701 tcctgagatt atacttgaaa tagttgagaa tctaaagaaa tacaatccaa aatatttggt
    41761 tgtggaccca gtaatgatat ctaaaagtgg atattatcta ttaaagccag aggcaaaaga
    41821 aaacttaatt aagtatttga taccacttgc atacataatt acaccaaata taccagaggc
    41881 agaagaaata acaggaataa aaatacataa tgtagatgat atgaagagag taggagaaga
    41941 aatactacaa cttggaccta aatttgtact tatgaaagga ggtcatttag atggagaagc
    42001 agtggatatt ttagttggta aaaatatatt taaagtttat aaaagtgaaa gaattgataa
    42061 aaaaaataca catggaacag ggtgcacatt atcttctgca ataacatctt atctagcatt
    42121 aggatatgaa ataacagaag cagtaaatct aagtaaaatc tatataacag aagcaataaa
    42181 aagaagtttt gatataggtc atggagtagg accagtacat catttttata aatttgagta
    42241 ataaaaaaat attggaggta gaatatgtat aatttaatta aagatgtaaa aaaattaaat
    42301 ccattggtaa ttcattatac taataatgta actataaatg attgtgctaa tgtaacttta
    42361 gctgtaggag caagtcctct tatgagtttt tcatatgagg aagtagaaga aatggtaagt
    42421 gtagcaaact ctgtagtaat aaatatagga actatgaact caaatatgtt ggatttattc
    42481 ctattagctg gaaaagcagc aaataagtac aataaacctg ttgttttgga cccagttgga
    42541 gtatttgcaa gcaaagcgag agcagaactt acaagtagac ttttaaatga agtcaaattt
    42601 agtgttgtta aagggaatgt atcagaaata aagtttatag gtggctttaa tgtaaggggt
    42661 aaaggtgtag attcatttga tgaagaagaa gattctactg agataataag aaagattgct
    42721 gaaaagcttg aatgtgtagt tgtagcaact ggaaaaatag atataataac taatggtaaa
    42781 ggtacatata aaataaacaa tggaactgat aaattaaaag gaattacagg aactggatgt
    42841 atgactgcta gcttgattgc aagttttatg gctgttactg agaatatatt agaagcagca
    42901 acaatgggtg ttcttacaat gtctttaagt ggggaattag ctaatttaaa taatccacca
    42961 ataggtacat ttaaagaaaa tcttatgaat gctatttatc aaatggatat agatgcttta
    43021 agtaaaaatt ctaatattga atttttaaat taataaagag taagggagac ttaaaatgat
    43081 tgataaagaa tctttaaaaa aatgtttaaa gttgtattta gtaactgact ctgaaatgct
    43141 taaaggtaga gacttttata aatgtcttga agatgcaata tcttctggaa taacaactgt
    43201 acaacttaga gaaaaaaatg cttctggaag agagttttta agaaaagcta tgaaacttag
    43261 agaaataact aaaagatatg gtgttaagtt tataataaat gatagagtgg atatagcact
    43321 tatttgtgat gcagatggag tacatgtagg acagagtgac attgatgtta gagaggttag
    43381 aaagcttata ggaaacaata aaattctagg agtatcagca agaactttag aagaagctat
    43441 ttgtgcaaaa aatgatggcg cagactattt aggtgtaggg tctatattca caacatctac
    43501 taaattagat gcaaaatcag cttcgtttga aactgttaaa gaaattaaag aaaaagtaga
    43561 catgccattt gtactaattg gaggtataaa tttagacaat atagataaac ttaaatgttt
    43621 agaaagtgac gggtatgcta taatatcagc tatattaaaa gctgaagata tctctaaaga
    43681 agtggaaaag tggacattaa aaatataaaa ttggctctgg tagtgaaaaa aatggaaaaa
    43741 tttgatataa tctaagagta aaatttaagc aattataaat gataaacttt tctacatata
    43801 ctttttaatc aagatatatg tagatttggt taaaaaggag ctataaatga ttaggatatt
    43861 gttagtgtgt gttggaggca tgtcttctac cctattggta aataaaatgg agaaggatgc
    43921 aaaaaaaaga aatatagact gtaaaatatg ggcagttgga gaaggagata taaaatcgga
    43981 gcttgataac tttgatattt tacttcttgg accacaactt agatttatgc ttgatgacgt
    44041 aaagtctata gttggagata gagctccagt atctataata gatatggtaa actatggcac
    44101 ttgtaatggt catgctgtgt taaattcggt tttagaaata ttaaaataaa taataaaagt
    44161 ggattctata taatagagtc catttttatt atttgttatt aataggctta aaacttagct
    44221 taccatttct ttttttaatc tccacatagc aagtgaaaaa cttgaacagc acaatattaa
    44281 aaatagtagg ataacaggca ttatatctcc aataagaata gtctcaccaa atattaagct
    44341 acgtaaagcg ttaataacat gggtaaatgg attgagcatc actgctactt ttaatcctcc
    44401 agataaactt tctataggga aaagagctga acttaaaaag aaaataggaa gtacaattgc
    44461 agtcataatt gtttcataaa ctatttcatt tggcaataat aaacttatag aataagctag
    44521 acttgataaa aagaaggctg tcataaatat cagtactatc ataagtagga ttccaccaat
    44581 gccagattct atccttactg agaagaatat acttactata cacaaaatag ttacttctat
    44641 gaaagaaact aagatagctt ctaataattg accaaggacg attgaatacc tgctgatagg
    44701 tgcaattaag actctataga aacttccact gttcttcatt ataaagttta taataccacc
    44761 actactacaa gaagaaaaca caaccagtac aatgatacct ggtagtataa aagcagtata
    44821 attattgata tttatattat tcatagtttg gtttgcaata gaactataaa gcacaagcca
    44881 aagaaaaggt tgtaatatag taactacaaa tgagattgga tttttaaaac gccacttcat
    44941 actacgccat aagatattta acattcccat tcctccttta agcttttttt agaacttgta
    45001 agtgctaaaa atacatcttc gagacttggc tctacaattt ctattgcatc aaattctata
    45061 tcatgttcta aaagccattt atttattaaa gtaaaagcag ttctactatc gtttacactt
    45121 ataaaaattg aattttctcg tacgctcata aactttacta aaccagtact tttaattgaa
    45181 tctttatatt tttttatatc ttcagtatta tgaaaagtta tacgtagtat attttgtcta
    45241 atgtaactac gtaaacttga tggtgttcct tgtgctaaat cctttccatt cttcataata
    45301 caaatattat cactcaattg ttcagcttct tcaagataat gagtagttag aaatatagtt
    45361 gtgccatact catctctaat ttttaataac atatcccaca tagattttct tgaatcgaca
    45421 tccataccaa cagttggttc atctaaaaat aggattttag gagatgatac catattcata
    45481 gctatgtcca atctacgttt tacaccccct gaatatgatg aagtaggata ttttaagtaa
    45541 ctagataagt caaacttatc aattaaagag tctattcttt gttttgcaat ctgtggttct
    45601 actttataca ttttactttg aaataccata ttttccataa gggataaatg ttcatcaata
    45661 gagatttgct gtgctacaca agcaatttga gtacgtatcc aagaaggatt atctactaaa
    45721 tcttttccaa acattgttac atttccagat gtaggtttgt aaaaggtagt caaaatattt
    45781 ataagagaag atttaccagc accattagga ccaagtaaag aaaaaatttc tccagcattt
    45841 acattaaagc ttagaccatt taaagctttc acaccatttt tatattcttt tactaaattg
    45901 tctatttcaa tcgctaacat atattattcc tcctctttaa gtggaaatat tatctctgta
    45961 atatagttat ctgggtttcc ttttatcaac atacctggtc ctttaataaa tacttctctc
    46021 caaggagttt gtatttttat attattttca tgaatatatt taaatatcat ttcatacgtt
    46081 tttggcaaat tactataaga gcctgtgtga gtaaaaaata aagcttttgt tttaggaaat
    46141 tcttttgtag ttatacccat acgatttgga gtttcagcag ttggaacaca taattccatt
    46201 tctgcaacac cagttttttg gtcaacatca tagtagcaaa agaatggtgc accgttagac
    46261 tttcctttta tagctttaaa tacatttgga aaatactttg ttgcctcagt cataggtcca
    46321 ttataacgca tggtagccac tgtgactgat tcgatatttt tgatttcaat tttgcaattc
    46381 atttgtttac ctcctaatgt gtgttcatat tcttactaga tttttaagca ttaatacata
    46441 taagattttg attagttgaa aacatataat cttatctcgt ttgcttgttt tcaagtctaa
    46501 cataacataa aatatatatc ctctcatttg atgctccatt ttgtaaagtt attatttaag
    46561 ctttacttta gattttttag caaattaaaa taaagcaatt tataaaataa gtatttacaa
    46621 attaagtgta ttaatcgtat aatacactta ataggatact agataaagtc tgaaataaag
    46681 gcgtattttt ttaaagtaag tgtactatcg tattaataca ctagaatagg aggaatcatg
    46741 aattttgaat tagataatac aactcccatt tatttgcaaa tagtaaagta tataaagaga
    46801 caaatagtaa caggagagtt gaaaccagga gagacaatac catcaagaag agaaatggca
    46861 cttaatctaa aagtaaactt aaatacagtt caaagagcat ataaggagat gggtgatatg
    46921 aatataataa atacttttaa aaattatcaa agcagtgtaa cagttgatga aaatatatta
    46981 aaaaatttaa aattagagct tataaatgaa tcactatctg tttttataga agatatgaaa
    47041 gcaataaatg ttagcaaaga agaagtttta aaaataatag aagataagta ttagtttttt
    47101 aacaaataga aaacttagtt tagaaagttt atttataagc atattggatg tggaggatag
    47161 taaatatgat agaaataaag aatgtcagca aaacatataa aaggatgcaa gggcttaaaa
    47221 taagaaagat agaagcttta aaaaatgtaa gttttaatat agaaaaggga aaaataactg
    47281 cattacttgg tataaatggt gttggtaaat caactatgtt aaaagcaata gcaggactta
    47341 taaacattga tagtggagaa attcgtatag atggagaaaa aataaatgaa aaggtataca
    47401 ataaattggc ttttgttcca gatgtacaat ctcactttag caatacaaca ataaaagaaa
    47461 catttgaatt tatggaaatt ttttattcaa agtggaataa agaaaaatca aaagaaatga
    47521 tggacatatt taaattggat gaagatgaaa taattgataa cttgtcaaaa ggaaacattg
    47581 caagagtaaa attaatactt ggattttgcc aagacccaga gtacatactt cttgatgaac
    47641 catttacagg aattgatttg tttaagaggg aagagtttat aggagtaata gcgcaatata
    47701 tggaggaaaa tcaagccatt attataacta ctcatgagat agtagagata gaaagtttag
    47761 ttgatgaggt tgtaattctt gatgaagggc agattataac ttcatttaat gcagaagaat
    47821 taagagaaag agaaggtaaa tctatattag ataagatgag ggaggtatat aagaatgaat
    47881 aaacttctat atctaataaa tgtagatttg agaagaagca ataagtttta tatagcttat
    47941 atatcatttt tttcattaat tacacttagt ttaaatttat tcgaaattaa taaatttaaa
    48001 aattctaaat ttattatgaa cttagcaata gaagattttg gaggtatatt ttatggacta
    48061 gggatactta gtaatcttgg ttttatccaa agtatattgt tctttgggat attgggactt
    48121 tttatatact atatttttat gtggaaaaga gagttctttt caaataatca aagtatttat
    48181 acacttatga tgttacctca aaataagttt aagatttgta tatctaaatc aatagccttg
    48241 ataacaatga tatatggttt tttgataagt cagacagcaa cattatttat atgtaaatat
    48301 atatttaatt tcatttttag aaatatgcct ataatcaata tgaactttgc caaagattta
    48361 tattatttaa acttgaagag tataccaatt gattttatct cttttatggc tatttatgta
    48421 tttttactac ttatagctat ttctattgta aattgctgta tattatttat cttttcctat
    48481 ataaataaat tttatgtaat tttaatcact ttgctgatta tagcaggctt atatttttca
    48541 acaatatata taaatataat cactttattt agaaaatatt ataatcttat aggatttcaa
    48601 tttaacaatt tagtagcagg gttattaagt tttttaatta taatgtttat tctaaatgga
    48661 atatcttatt tgctaattaa aaagaaagta tctctttaag gaggggtggt atgaaattaa
    48721 aatctaaaat atctatattt attatatttt tgactttagc aatatatttt agtataggtt
    48781 tattaaataa tgaagataat ttaaagataa aaactataaa aggtgactct aatgaattag
    48841 accatataaa cataaatttt ggatatgctt tgaatggata tataaattca tattatacaa
    48901 taaatggaag caacaaaacc aaaaagactg aattaataaa actacaaaag gatagtaaga
    48961 acacaaataa tttaattgat gtatctagtg gttcagattt aattgctcat gttggatttg
    49021 aaatgaatga ggagtcaaaa gatataaact ttgtgataga tgagaaaaaa gaagatttag
    49081 aaaaaggtgt atttccatca gaaggtagtg ataaagaaat tgaatatgaa aaattagata
    49141 tgaataattt tacaaggaca agatataaag tagataaaag tacagatgat tattatgcaa
    49201 agatactagc atcaaaaaga tataaaaaat acttatatgt atctgtactt tatgctacaa
    49261 gtgattcatc agaaaataac aaagaatatt caagtactat agatataata aaaattaatc
    49321 cagaaaataa aaaaatcaag actataaaaa gtataaattt aagtaattat ttttccgaaa
    49381 aataccaaaa catagagaac ataggtgtaa gttatgctag caaatatgag gataagatgt
    49441 atattttagt aaatgtacta aatacaaaca taggtgataa tagtaattct aaatttcatt
    49501 tacttgagta taatcttgaa gaaaataagg aaagcataat ggaattaaaa actgatagca
    49561 aaatggcttt agcaaaagca aagttagaag ctaataaatt atatatggta atgtcgaatg
    49621 attatgataa tagttcaatt acagagttaa atttacttac ctataatata ttagataaaa
    49681 gcttagatga agataaatat gttttagata atagagaaaa aagaggaatt agaagtattg
    49741 aaattgattc aaataaaatc gttgcatata catcaaggct aaactctata ttatcttggg
    49801 atgataaatt tatgctatat ataataaata aaaattctaa ggaaattgta tatgaaggag
    49861 aattacaatt taacagttca aatagtatgt tagaatttga aataaagtag gtgagttgat
    49921 gaaagattta ataagattat tcgaatttga attaaaaaga aatataaaga attatatatt
    49981 tataataatt tgttgttgtt cttttgtaat tttaaacata gtaaaaaatt taaatgacta
    50041 taattacata atagaaaatg cagttaaaag tgaacagtta acaaaaatcg gaaatacgat
    50101 agaaacggta aatgttacag gtttctcaag ctttagaaat ataatgggtt ctacagaatc
    50161 gtggtttatg tttggtataa ttgcatgtat ttgctatgct ttttttattt ggtatagaga
    50221 ctttaatggt agaagtaaat ctatatatac acttataatg ctacctaaaa atagaatcaa
    50281 tatttatata tctaaattat taaatatatt atttctagtg tatagctata cagtagtact
    50341 tacaatatct ttgtttattg caagtaagtt gttgcctaga catatgcttg gtaatgtcac
    50401 aaactatggt tttgtacaag aaacaatata tgagttaaaa atgttacttc catatagctt
    50461 tgaaatacta tttgtagagt atatattttt attgatagga tttgtatctg tagtatttac
    50521 atcaattctt ataaataaat ctatttataa agtaagcact ttaataagtt ttctattttt
    50581 agttatagaa attttagtgt ttataatttc tatagaattt attatacctt acgaatacag
    50641 tgatgtattt actgttctat attcaagtat aaacatattt gtatgtagtc ttatatctaa
    50701 taaacttttg aagcaaaaga ttgactttta ggagggatat tatgaaagca aaaaatatag
    50761 gaaaatatac aatatgtttt ataatagtat gttttttaat gattactatg accttaatat
    50821 tgagttattt taataaggat aaactatctg taaaaacttt aaaaggcaat gtaaaagata
    50881 tagggagagt aagtgtagta tatacaccta atataagcaa attttcttat gaggaggtaa
    50941 ttataagtaa aaatggagta agaaataata caaacccaaa atcaagaatt agaaatgatt
    51001 atttaaatat tgacaaaaaa tacagtgact ttgttaatga taaaggaaaa gaatttatat
    51061 ataaagatga tttgaatata ggagaagcgt atacctctgt taagtctgat ttagtacaag
    51121 gaatgaatgc tttaaatgat tgtataaagg tttcaaataa aaatttaaaa agtggggtag
    51181 aagaaaattt tgatattaat ttaaataagt ctgattttaa agaaaatagt tggaaaacct
    51241 tatttaacaa aatatataaa ggtaatttat atatggtaat tagtgaaact aatatttcag
    51301 gttctaaagt caataatatt tgtatattaa agatagat
//
LOCUS       DAMJVK010000014        51247 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.014, whole genome shotgun sequence.
ACCESSION   DAMJVK010000014 DAMJVK010000000
VERSION     DAMJVK010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 51247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51247
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.014"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            116..745
                     /locus_tag="PC414_000853"
     CDS             116..745
                     /locus_tag="PC414_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HDF2463262.1"
                     /translation="MDEKITKNNYNVPFDYVRKKLKELDPHTISNLSNIPFNNDLNRF
                     TLNFFDRQYIIDYPSGEVWNSNGSLCNNFELKILIVRYLINAKGIPRTNKYVTFKEIP
                     GGNVYYPNFLNRTLYRLSEVFTSQIDKYKLVMEKIGAEKVNMGDLAYRFTYMGNTSMV
                     FILWFGDDEFPPNSNILFDSNIVYYFNAEDLAVVPDTAINAILNKINQL"
     gene            813..1646
                     /locus_tag="PC414_000854"
     CDS             813..1646
                     /locus_tag="PC414_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HDF2463263.1"
                     /translation="MNVENQSLSDIEIILSHRYDNGADYWTTPDKRLIKGSPFSTLDS
                     VLYLLELGVNPDDTLLKECADLIFSTWREDGRFKLYPNGGIYPCHTANAANVLCHMGY
                     ASDIRMQKTFQHFLDTQYTDGGWRCNKFSFGRGPETEYSNPLPTLNILNAFRFSNYLN
                     KESKLDKAVDFLLDHWTIKKPIGPCHYGIGTLFMQVEYPFRNYNLFLYVYVLSFYNCA
                     KKDNRFLEALKILESKMIGNKIVVERVVPKLSKLSFCKKGESSEIATTHYYEILKNLE
                     K"
     gene            complement(1742..2530)
                     /locus_tag="PC414_000855"
     CDS             complement(1742..2530)
                     /locus_tag="PC414_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463264.1"
                     /translation="MCILELIKFELKKIELKHVLIFVIISILCIFITGILDYNELIFY
                     NDVPIMDTYSIAGWLGIIKISDVFIPIMSAITIIYVFSKDYSGNINEILTLYNKKKYN
                     QFIVFRWCVILAIYTVLLLISITIVYTFTSVKGFNIDKIDFVYSVKPIDVFLKTFPTL
                     LWYTTFPLMILTLTKNRFTTIAILITYTFADIFFILHMYPFVSMINVNGFYIQKMFYM
                     PNNIDVRFNELKNYFFINRGSLVVISIIIVSYIARKSTVLKKSK"
     gene            complement(2531..3403)
                     /locus_tag="PC414_000856"
     CDS             complement(2531..3403)
                     /locus_tag="PC414_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2463265.1"
                     /translation="MINKLEIRNLNFNYKNKKALDNINLTLIDGVVALLGPNGAGKST
                     LMRLLVTLYETSIGEIELNNIKYSKNNEKIKANVGYVPQDFDMYNNINGQEYLEFVAK
                     MRGVSKNDLKKHIQKVVSKVNLDKFINKKIGTYSGGVKRRLGIAQALIGDSKLIVMDE
                     PTVGLDPEQRNEFRRLLPEISKNSIVLISTHIVEDIQFNCDKLIILNQGKILYDGTIN
                     KFIDMVEVYSVVVSNDEFEYLERNIDIIDYRRVKDGVQVKYIGKNTEKLKDSLRIDTT
                     LQDAYINFLHKELK"
     gene            complement(3418..5127)
                     /locus_tag="PC414_000857"
     CDS             complement(3418..5127)
                     /locus_tag="PC414_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463266.1"
                     /translation="MSSIKAISDFITKIIRKNILFAITIIIINYHLIQRWTHISNRGI
                     SFNDYLSLSVYTCMTISILTMLNVIFYMRKSYDTVKILCRNNFDYFLGISVAVTRNNF
                     IFCCIPIIYAIAYSSLIRTLSIVTIKSTIFILIFEWFLPLTILSIVISFISVIINNLI
                     LKITLSSVFLYLTSTNMLDRVIDIKEPFFKALFKSLNIFEDQSYAYYSDFVGKVNNSA
                     YVIDKIIPTLFAITLIIVAYLILNDIRKKQIKSLIVIVVYSILFIGLNYISIDMSTYR
                     DEERSYGEYLKKSNISDIKIKSHDMKIDFKSKSNIKDRIVLLNTSNEELTNINMILDE
                     IFDIKYIKINSENIKFNKKKDLIEVNLKESLEPNDEVTLDIEYEGYINVLTSWNQNKY
                     IATKDDIMLPPGSLAWYPKVNQSSCMAYKINLSSKNNVYSNLPLLNKTSNFLKKDYIF
                     EGKSNDIAIYAGEFKEEVKDDIKIIYPSDSNVNMDIEEKIKFLIETRNEILGDSKASV
                     DAKKYVDEVDNVMESIKRKKINMILVADLPSNEFLYEDSKGEKISITIYHWLSGNILI
                     IDK"
     gene            complement(5274..5855)
                     /locus_tag="PC414_000858"
     CDS             complement(5274..5855)
                     /locus_tag="PC414_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HDF2463267.1"
                     /translation="MFKRLSYKFANILVNNEIVESEDFEIYRYGFETLIYFIVNILVA
                     LFIGIIFDRFIHTVIFLSCYCTLRQFTGGYHARNYKECTLTFAVIYLITIFSANNIDI
                     NKYKYLLVLLMIISILTIYKLAPLEHRNKPLSESEKKHYRKTVQKILFVIICLIILCK
                     ILNIFQQYVIYAIISIYWIAILIYIGMKVNSDQ"
     gene            complement(5914..6054)
                     /locus_tag="PC414_000859"
     CDS             complement(5914..6054)
                     /locus_tag="PC414_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HDF2463268.1"
                     /translation="MKKIALNLLKNISALSFGIAVLSANSASSWVAHQAKEPQALQKL
                     KK"
     gene            complement(6066..7382)
                     /locus_tag="PC414_000860"
     CDS             complement(6066..7382)
                     /locus_tag="PC414_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF2463269.1"
                     /translation="MLTNLSVGFWNIINLVSGTIDWIIFFLVIHLVGKRKISNIRYIF
                     TTIIIIIFMAYINVVKVFPNIKILLCMVIGISFYITSYEDKIYKSIIVSLLFWLGLMV
                     AEGSAVGLVVFINKLNNINITQHENLFRIQAIIMSKVFLFIGFILFKYFKLSLDFKPK
                     DMILIGIPILSNIVSLLLIFEYNLRVNTITTENLFIFIFAILLILSSSIILLIVIGKI
                     VQDDKLNLEYELINERIKTNYRNYENINEVHDRLKYVYHDLKNHMICIKNYDTKEEII
                     SYINNLEFQISEFEHFKNTGNKTLDLILAEKISICKKYDIQIEDNINISKLNFIKNND
                     ICSIFANSLDNAIEACMDINNEIEKRIEVKATYINKFAIIKFINTKVNDIKLIDKRIQ
                     TSKDDDKIHGIGLASIKYIVNKYGGETIVNYSDNEFILKIMIPIKS"
     gene            complement(7376..8086)
                     /locus_tag="PC414_000861"
     CDS             complement(7376..8086)
                     /locus_tag="PC414_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2463270.1"
                     /translation="MINIGICDDELHYRIKIKDILSEILSSYPINYNIYEFSAGEELL
                     NNYPKDLDILIMDIQMKTINGMDTARKIREFDHKLEIIFVTSFVEFMQEGYEVKAYRY
                     ILKPINKEKISKSVLPCINEMMKKRNNYLTINVKNYVDRIKIDSITYIETDRPNILIY
                     THDDMYITKMSISKIEKILNEYGFFRCHNSYIVNLKLVESMSGSTVIVDGKSIPISKY
                     RVKGLKLAITNILGDIVC"
     gene            complement(8525..9100)
                     /locus_tag="PC414_000862"
     CDS             complement(8525..9100)
                     /locus_tag="PC414_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1062 domain-containing protein"
                     /protein_id="HDF2463271.1"
                     /translation="MKKITWEVEYISQLPVIRYCKKCGKKTEYICSGLFRVNAQHKYL
                     DIWLIYKCSNCDSTWNSTIYSRVNPKSLNPEILEQFHTNDYNLVKQYAMNMELLHRNG
                     AEVGIPEYKIVGQEININDLTQVHIISKYPCQLKVSTLLREKLGLSRKVFDEMLDCGI
                     IRSNSGVDLKKGKLNYEIMLDIGKEKQKSSN"
     gene            complement(9393..9671)
                     /locus_tag="PC414_000863"
     CDS             complement(9393..9671)
                     /locus_tag="PC414_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463272.1"
                     /translation="MSYKKAIHILPEELLELIQEYVDGECIYIPRKSNNKKEWGSNTP
                     TREELSIRNMQIYEDYQVGYDLQYLSEKYYLSLKSIQRIVLQEKRKDI"
     gene            complement(9980..10741)
                     /locus_tag="PC414_000864"
     CDS             complement(9980..10741)
                     /locus_tag="PC414_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HDF2463273.1"
                     /translation="MKNKIEISMNNFVKNYHKSNNITTKWQELLFAYASAEDDMFQKL
                     KSVVGTSHAMPKDFLENGKTIITYFIPFDESVNNSNIGGFECSEIWARAYIETNMLIS
                     NLNDFLCEELEKMGANSYAIPGSYNFDSEKLVSDWSQRHVAFIAGLGTFGLNNMLITE
                     KGCCGRIGSIITSLELEPTKRPENEYCLYKHNNSCKKCVNKCVKEALKEDGFNRNKCY
                     EMCMINEKKYTYIGGCDICGKCIVGVPCSVINPSR"
     gene            complement(10910..12985)
                     /gene="bclA3"
                     /locus_tag="PC414_000865"
     CDS             complement(10910..12985)
                     /gene="bclA3"
                     /locus_tag="PC414_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA3"
                     /protein_id="HDF2463274.1"
                     /translation="MLLIMSRNKYFGPFDDNDYNNGYDKYDDCNNGRDDYNSCDCHHC
                     CPPSCVGPTGPMGPRGRTGPTGPTGPTGPGVGATGPTGPTGPTGPTGNTGNTGATGLR
                     GPTGATGATGPTGATGAIGFGVTGPTGPTGATGATGADGVTGPTGPTGATGADGITGP
                     TGATGATGFGVTGPTGPTGATGVGVTGATGLIGPTGATGTPGATGPTGAVGATGIGIT
                     GPTGPTGATGADGATGVTGPTGATGATGADGVTGPTGATGATGFGVTGPTGPTGATGI
                     GITGATGLIGPTGATGATGATGPTGVTGATGAAGLIGPTGATGATGADGATGATGATG
                     ATGPTGADGLVGPTGATGATGADGLVGPTGPTGATGVGITGATGATGATGPTGADGLV
                     GPTGATGATGADGVAGPTGATGATGNTGADGATGPTGATGPTGADGLVGPTGATGATG
                     LAGATGATGPIGATGPTGADGATGATGATGPTGADGLVGPTGATGATGATGATGPTGA
                     TGATGATGPTGASAIIPFASGIPLSLTTIAGGLVGTPGFVGFGSSAPGLSIVGGVIDL
                     TNAAGTLTNFAFSMPRAGTITSISAYFSTTAALSLVGSTVTITATLYQSTAPNNSFTA
                     VPGATVTLAPPLTGILSVGSISSGIVTGLNIAATAETRFLLVFTATASGLSLVNTVAG
                     YASAGIAIN"
     gene            complement(13052..14128)
                     /locus_tag="PC414_000866"
     CDS             complement(13052..14128)
                     /locus_tag="PC414_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2463275.1"
                     /translation="MITISLCMIVKNEEEVIGRCLECVKDIVDEIIIVDTGSTDSTKE
                     IVSKYTDMVYDFEWIDDFSAARNFSFSKASKDYTMWLDADDIILEADREKLLKIKESL
                     DTSIDIVMAKYNVSFDENGNPTLSYYRERLFKRSSNHKWVDPIHEVVPLFGEVFYSDI
                     AISHKKLHRQDPLRNLRIFEKMISEGKTLEPRHQFYYSRELYYNARYKEAIEGFTKFL
                     DSSRGWIEDCISACRDLATCYYLINDEKSALYSLFRSFEFDEPRAEICCDIGKHMFDR
                     QKYKEAIFWYKVALTRDKNDTDGGFKSDDCYGYIPSIQLSVCYDRLGESDKAIYYHEK
                     TKEIKPNDSAVLHNENYFSKFKNS"
     gene            complement(14440..15024)
                     /gene="clpP"
                     /locus_tag="PC414_000867"
     CDS             complement(14440..15024)
                     /gene="clpP"
                     /locus_tag="PC414_000867"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDF2463276.1"
                     /translation="MSLVPHVIEQTGQGERSYDIYSKLLKDRIIFIGEEINDAIASLV
                     VAQLLFLESEDPDSDIIIYINSPGGSATSGFAIYDTMNYVRCDVSTICIGMAASMSAF
                     LLAGGTHGKRCALPNSEIMIHQPMGGAKGQATDVKIAVDNILKIKERLDKILSENTGK
                     SIEDIRRDTDRDNFMTALEAKEYGLIDYIMNRNE"
     gene            complement(15021..15479)
                     /locus_tag="PC414_000868"
     CDS             complement(15021..15479)
                     /locus_tag="PC414_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2463277.1"
                     /translation="MEIIDYIEENLTENLNLDKIAYIANYSKYHLHRMFTNVVGCTIH
                     QYIQRRRLTEAARQLIFTDKYIIDIAITVGYSTHQSFTLAFRNLYKESPQVYRNKDEF
                     YPIQQRFVLNLNHQIQSEKCISYANMNYKQRRDEINSYLNFNINCEVKVA"
     gene            complement(15702..17117)
                     /locus_tag="PC414_000869"
     CDS             complement(15702..17117)
                     /locus_tag="PC414_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF2463278.1"
                     /translation="MWITINRNSNISLSRQIYCEIKDMILDGSLKFEQKLPSSRTLSK
                     ELSVSRNTVLDAYNQLIAEGYLETRNGYGTIVAKGISGHKVDFKLNDTFRDEKDLHTN
                     KQKNYIDFRSGVPELKLFPRKELSKLYKQILYDLSDSDFRYGSTAGVWVLRVEISKYL
                     FRTRGIRCNPKNIMIVSGSTQGLSLISQLLYKSNKKVAVEDPIHKGLLKVISSIGYSV
                     VGIKVDDKGMNTNLIKSIKGISFVYTTPSHQYPLGGILPIQRRLELIKCATENDYYII
                     EDDYDSEFRYEGQPISSLYELSPNKVIYIGSFSKILSPAIRVGFMLLPDNILESYKTL
                     KMYSDVHTETILQHVLAEFIGNGGLEKYIFRMKKLYNRKRKYLIKVLNTEFKNEFKIK
                     GEAAGLHIVAHFYNVKFTESLIHKIFEAGIKVYPIQQYAIENILEYDYEIILGYAHLS
                     FDEIEKGVKILSKIIHMNQGT"
     gene            17232..17900
                     /locus_tag="PC414_000870"
     CDS             17232..17900
                     /locus_tag="PC414_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463279.1"
                     /translation="MLEISHKVKEIYPNIKFGVMVINITHSKPNKENFLVLKNSTIKN
                     IIEQHPEYNRKEKIKTEPVSSYIKYYKKFKKTYPVLLQLESILLKSKGIPDVGVTIES
                     MFLAELKNLLLTAGHDLDKMELPLKIELANGSEHFYGIGGKEHILTKDDLFLSDNIGI
                     LSSILNGPDNRTAITKDTKNIMYFVYGPDKISEKQIQDHLNDIKHYISSAFPDLKVNS
                     IDIF"
     gene            complement(18076..18594)
                     /locus_tag="PC414_000871"
     CDS             complement(18076..18594)
                     /locus_tag="PC414_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2463280.1"
                     /translation="MNFVELAKKRYSCRNYQDRKVEKEKLEKVLDVARIAPTGGNRQP
                     QRLIVIQEKEGINKLSKAANIYDAPLAILVCGDKDKVWTRPFDGKQLTDIDTSIVTDH
                     MMLQATELGLASVWVCYFNPDIIREEFSLPDNLEPINILLMGYESKIPESPERHEKTR
                     VPLSEIVSYETL"
     gene            18746..19069
                     /locus_tag="PC414_000872"
     CDS             18746..19069
                     /locus_tag="PC414_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2463281.1"
                     /translation="MKKCLDNYSCPIEATLALIGGKYKTLILWHLKDTILRFNELKKL
                     IPKATPKMLTQQLRELESDGLIIRVVYPVVPPKVEYSLSDFGKSIIPILDSMCDWGSD
                     YLENL"
     gene            complement(19074..19658)
                     /locus_tag="PC414_000873"
     CDS             complement(19074..19658)
                     /locus_tag="PC414_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HDF2463282.1"
                     /translation="MKVLVFLAKGFETMEFSVFVDVMGWARNDYGHDIDVVTCGFKKQ
                     VMSTFNIQVLVDKTIEEVCVDDYDALAIPGGFEEFGFYDEAYDSSFLNLIREFNSKEK
                     IIASICVAALPVGKSGVLKNRKATTYHLKNGKRQRQLSEFDVNVVNEPIVVDKNIITS
                     YCPETAPHVAFKLLEMLTSKEQMDEVKLAMGFKL"
     gene            complement(19707..20162)
                     /locus_tag="PC414_000874"
     CDS             complement(19707..20162)
                     /locus_tag="PC414_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HDF2463283.1"
                     /translation="MDEIDLKIINSLKKNSRASTSEISRQINLSVPAVSERIRKIEQS
                     NLIQKYTIQINREKTKYKLLAFIFINTDLSKSMKEFKKTILEYDTVLECHHISGEYDY
                     LIKVLAEDTSNLEEFISVTLKEIGGIQKANTTIVLSTIKEEFNTIKDIF"
     gene            21044..22582
                     /locus_tag="PC414_000875"
     CDS             21044..22582
                     /locus_tag="PC414_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDF2463284.1"
                     /translation="MSLLKVTNLSQCFMDKSLYEKANFDLFKGEHIGVVGQNGTGKST
                     LIKILLGEVVPDSGEIKWQPNINIGHLDQYAEINRDTTISLYLHTAFEELYKIEKEMN
                     LLYQKSAISENEQYLIKASDYQEQLIASNFYSIDNEINKIANGLGLDSIGMNRVVGEL
                     SGGQRAKVILAKLLLSNHDVLLLDEPTNFLDKEHVEWLSNYLNTFNGAFIVVSHDFDF
                     LEKISTGILDIEFGMIKKYHGKYSEFLKQKSHLREDYIRRYQAQQKKIEKEETFIRKN
                     KAGVNSKIARGRQKQLDKIERIAPPSFTGKPNIQFSEIEISAQNALTITNLEVGYYYS
                     LLPKLNFSVDGGQKIVITGFNGIGKSTLLKTLVKDIPRISGDFQFSEQVKIGYYEQDL
                     KWENPDKTPLQIVADKYPKLNTKEIRRHLARCGVKEEHVSRSVSTLSGGEQSKVKLCC
                     MMLSPCNFLILDEPTNHFDAETKDALQNALKQFRGSIILVSHEEKFYKGWIDKVFNIE
                     KQLV"
     gene            complement(22854..23567)
                     /locus_tag="PC414_000876"
     CDS             complement(22854..23567)
                     /locus_tag="PC414_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2463285.1"
                     /translation="MDTCFNKKILLVDDEKDIVDLIEEVLINDGFKNIIKAYNGLDAI
                     SLCKIACPDVVILDIMLPDIDGIEVCKKIREFSYCSILFLSSKNDDIDKILGLSSGGD
                     DYITKPFSPREIAFRVKAQLRRQQYQSIVPSDSEVIKIGDITIDIEGNRVYKDRNEIE
                     LTGREYHLLSYMAQNVNKIIGKERLYEQVWGVYSSICDNTIMVHIRHIREKIEDNPSN
                     PKILITVKGLGYKLVNRID"
     gene            complement(24015..25394)
                     /locus_tag="PC414_000877"
     CDS             complement(24015..25394)
                     /locus_tag="PC414_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2463286.1"
                     /translation="MSDVYYSENHEQDNLYEQDNLLAQRFNLFTLLKFAFPTIFMMIF
                     MGLYTIVDTIFVSRFINTNALSAINIVCPIINLIVGIGTMLATGGSAIVARKMGNGDI
                     EGANKAFSLIVLSGVVIGVIITGIGIVFMDYIIIWLGASEVLIKYCRSYLFIILVFAS
                     ANILQVLFQCLFVTAGKPQFGLWLVLGAGCANIILDYVFIVLLQMGISGAALATGVGY
                     LIPTVSGMVFFSKNKGLLKFCIPRLDKRILFEACFNGSSEMVGQLSTAVTTFLFNAMM
                     MKLIGEDGVAAITIIIYSQFLLTSLYIGFSMGVAPIISYNYGSENNTQLNHVFKICIL
                     FISVASLLVFIFSISCSPIIVEIFSQRGSRVYEIARSGFYIFSFSFIFCGYNIFTSSM
                     FTALSNGKVSAILSFLRTFGFITIGLLILPNILNINGIWLAVPSAEILTLFLTIFCIC
                     KYKNRYKYM"
     gene            complement(25679..26473)
                     /locus_tag="PC414_000878"
     CDS             complement(25679..26473)
                     /locus_tag="PC414_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HDF2463287.1"
                     /translation="MMKKNLKIIFMLLKMKLSKMMVFRFDFFGAFFVDSSLFILQLLM
                     FNSIYSHVDSIGGWQQGEMLIFIGTFSLINAINMTIFFFGIYDIPRKIQEGELDYYIT
                     KPLDPLFRLTFENINIGSSPLILASILIVLYGISELNSEITLVMIIIYIFMVLLMTLL
                     FYDVCIILRTVSFFVISSSAIMRLEDNLLPMNMKIPGVIYEGVFKVLFYLILPYGIMS
                     TIPTQILAGTITINGLIYSILIVFLFTLFMWRFWKLGMRHYKSASS"
     gene            complement(26470..27333)
                     /locus_tag="PC414_000879"
     CDS             complement(26470..27333)
                     /locus_tag="PC414_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HDF2463288.1"
                     /translation="MSIMSNYNEASSNDSMFYKFKQSIRRYYEVAKINFKVQLIWRFD
                     VVVNMVFTIVKILFAYILWKAIFGENKIVSGFTFNSMLSYYIISSFLAGIDMSKSTSE
                     EICNRVKLGTFSKYMIIPINVQNYFIAKNFGMTCFYFVFNMISAFVWIFIFKIDFAFT
                     SNPVQILISILMIFLGFIFMMKLHFFIGILSFKFLEISFFKMIIENIVEFITGTMIPL
                     VLLPETIVTVMKVFPFYYTTYLPSMLLIGRNSNEAIQGLIILALWVLFFSVVNKVMYK
                     RMRTLFDGVGI"
     gene            complement(27311..28306)
                     /locus_tag="PC414_000880"
     CDS             complement(27311..28306)
                     /locus_tag="PC414_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2463289.1"
                     /translation="MSIIELNELTKAYRRFEKKAGFMGSVKSLFNRKYTEKTAILSFD
                     LKIEEGEFVGLIGQNGAGKTTLIKMLTGIIAPSSGEISVMGYYPNKLENNFKKQYAVV
                     MGQKSQLFFELTPADTFLLFKELYDISNEEYKKNLDYFIELFEVSDYLNVQVRTLSLG
                     ERMKMELIVALLHNPKILFLDEPTIGLDAIAQKQIRYFLKEVNETKGTTIILTSHYME
                     DIKSLCSRSIVVENSRKIYDGSTEKLFNHFQKYKKITVSFSNETNIVFPKDCKVVEQT
                     NHKISVIVPKERGNETIKWIFDKYDVKDVMIEEEDIGSIVERIYKKGSDEYYEQL"
     gene            29009..29965
                     /locus_tag="PC414_000881"
     CDS             29009..29965
                     /locus_tag="PC414_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF2463290.1"
                     /translation="MENNTIQKQLRDLSKKIWHIYILNPEEENLKFIISLLDDNLALI
                     GTGKHEFYETLEEFIIGLEKDQTESEQIDFEIIDEWYSCTKVTDEAFIVYGTLWVIEK
                     DYLEKDIYVEMDTRFTMVYRVTDDMIKLIHVHHSIPNFDQMKDEYYPKTITEKAQEAL
                     AMANEFKLKSEKDLMTGLYNRHFFEVHIKEMLKTVSHGYFYIFDLDNFKQVNDIFGHV
                     RGDEILIHFSNILTKVFVNDAIIGRLGGDEFAVFEYSNQSKENVENKAKKVLELCLES
                     NSCSIGISKLENNSDTFQSLYKQSDYALYYSKRNNKGNFSWF"
     gene            complement(30358..31248)
                     /locus_tag="PC414_000882"
     CDS             complement(30358..31248)
                     /locus_tag="PC414_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463291.1"
                     /translation="MKKNILTTMITMIIILMMSMTVMAEPYQEDETSSLKKQSANERN
                     QTNIGTMDGRVQTNAISKGANYDPKYPLKGMLTQLGLNTTGKEGWDLLYGGNPNGVEL
                     RADGKYYYINKGMEGLGFYAAALSGGSYQRIGFGKLDPIEVIADGNWISYKQAQDTLT
                     VIRNFLNSFDWRNASEMERANRAAKLVTEAKYADSKYCNIVYGNLVDKRGVCGSFASS
                     FHLLTRLMGMDSLSILNPSLNHAWNYIQIDGKWYRFDGSEISAFGGSLDFDYRKLKDA
                     TREMTTYYDAKALSILGFNQ"
     gene            complement(31426..32124)
                     /locus_tag="PC414_000883"
     CDS             complement(31426..32124)
                     /locus_tag="PC414_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463292.1"
                     /translation="MKISSQYRSQYSFRYESNINNTRINESMVKKNETVGKDTYLSNI
                     MKQKQELNDRIRDLKYRQEVYTKKINDAIKNLCKSEIRETTNNFSNIEIGIKNSIIEE
                     KNKSTMLDENSTYLNTNDEKESLITKESNEKIEEEILNDEKLEELEQKKDYKEDSNKK
                     EKVSEDLSLVGKTREELENMLKNFINLTQEEIMKLESRIEKLDKNAEEYKQNSKTNIF
                     DKTDEQKKHINVLI"
     gene            complement(32660..33727)
                     /gene="rsgA"
                     /locus_tag="PC414_000884"
     CDS             complement(32660..33727)
                     /gene="rsgA"
                     /locus_tag="PC414_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HDF2463293.1"
                     /translation="MNGMKQINLQDYGLNRNFITQSMEYHDLYLARIISQEKNFYQAV
                     CVNGVLFAEVSGKLRFEATIQSDFPTVGDFVMLDRCNNKNGNAIIHHVLHRKSLFVRK
                     AAGKVNEEQVVAANIDIVFLCMSLNKDFNIHRLERYLSLTWESGAIPVVVLTKSDLCD
                     NVHRKLQVVNSIALGVDVVVTSSIEPDGYKQISKYIKPGCTVALLGSSGVGKSSLINC
                     LMGGEFLNTKEIRIDDDKGRHATTRRELFLISGGGIVIDTPGMRELGMWDVSEGLDKS
                     FADIEAYFGKCHFRNCTHTSEPGCAIWEAIAHQELSPKRWESYRRLKTEAVFADNKSE
                     YLAKKEKKFKNISKINKKIRR"
     gene            complement(33724..34125)
                     /locus_tag="PC414_000885"
     CDS             complement(33724..34125)
                     /locus_tag="PC414_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HDF2463294.1"
                     /translation="MNYKNRKRAYYQENSCMDNFICKNCGCLVVPEGAGTQHRNHCPH
                     CLHSLHVDIIPGDRAADCDANMEPIGVWVRKNGEWAIIHRCTRCGHLSSNRIAADDNS
                     VKLMSIALKPLAQPPFPLERIIEKLEKEETE"
     regulatory      34922..35018
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            35072..35248
                     /locus_tag="PC414_000886"
     CDS             35072..35248
                     /locus_tag="PC414_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463295.1"
                     /translation="MFRDKMDRCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     gene            35658..36296
                     /locus_tag="PC414_000887"
     CDS             35658..36296
                     /locus_tag="PC414_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463296.1"
                     /translation="MSNQRKDFDITNDMFQESIPITTKIILEDMPSNDELNHVFSKGC
                     ERKMKKRKIVLITLLLIGVLLLGSILYNLFLGKTANISMLKESWNFDIPIPNKEIEVF
                     DTQDSINGDGQSYFIQGFSEKNFKKVFNLKGGIVVSKDNINEIEKYIDKFKRDSVNIN
                     KSNKNKIEEDFKKYKLEVKKDDKYIYKRNYENYVVLIIKKDEQKLYSLIWNQ"
     gene            complement(36769..38127)
                     /gene="rlmD"
                     /locus_tag="PC414_000888"
     CDS             complement(36769..38127)
                     /gene="rlmD"
                     /locus_tag="PC414_000888"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDF2463297.1"
                     /translation="MLSKNEMYIVDIVDIGQGGVGVGKYNGITVFVEGGLIHDKVKVR
                     INKVKKNYAVGDIVEIIEKSPFRVKRVCSDNLRDCGGCQIQDLDYNKQLEMKTNEVKQ
                     VLSRIGKLEDVEVHDTIGMDNPYRYRNKAQFPIQKNNGIPIIGFYKKKTHDVISTEKC
                     VIQHEINDKIVKIIKTYIRAYKVSIYDEKTHTGLIRHLVTKIGFTTKEVMVVLVANGN
                     KLPYLKELASVLKENIPGFKTLVVNVNQKDTNAILGRENIVIYGDGKINDYIGDLVFE
                     ISPLSFFQVNPVQTKVLYDKALEYANLNDTDTVFDIYCGIGTISLFLAQKAKKVYGIE
                     IIDDAIKDAKINAKLNNIHNAEFYVGKAEEIVPKMYREGKTANVVVVDPPRKGCDEKV
                     LETIVSMNPERIVYVSCNPSTLARDLDYLNVHGFKCVEAQPVDMFPHSTHVETVAKII
                     KQ"
     gene            complement(38302..40062)
                     /gene="pyk"
                     /locus_tag="PC414_000889"
     CDS             complement(38302..40062)
                     /gene="pyk"
                     /locus_tag="PC414_000889"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDF2463298.1"
                     /translation="MLNNVKKTKIVCTLGPASQSEEVLTQLMQNGLNVCRFNFSHGSH
                     EEHKERIDMAKKVREKLNKPVAILLDTKGPEIRTGNFEDPEVFLEEGQKFTITMKDIM
                     GTKEMCTVSYKGLAEDVKSGDSILIDDGLVGLRVKEINGEDIVCVVENSGIVKNHKGV
                     NVPGVKINLPAITPKDISDIEFGISQGIDYIAASFVRKASDVLAIREVLENNNATDIQ
                     IISKIENQEGVENLDEILKVSDGIMVARGDLGVEIPTEEMPIVQKMMIKKCNELAKPV
                     VTATQMLDSMIRNPRPTRAEVTDVANAIYDGTDAIMLSGETAAGKYPVEAVKMMATIA
                     KRTEETLDYDRLLKENGTNNVTVTDAISHATCTTAVDLNASAIITSTSSGYTARMVSK
                     FRPKSPIIATTNNEKTMNKLALTWGVYPVKSSVAGNTDEVIEKAIEAARQADYIDNGE
                     LVVITAGVPVGVSGTTNLIKVHVISEEIVQGIGVGTQTVEGKVRIIKNGNACVDFNEG
                     DILVSTATDAEMNNYIEKAGAVVTENGGMTSHTAIVGINLDIPVIVSAADITNLVKDG
                     EVVTVDASRGVVYRGSTRVL"
     gene            complement(40114..41073)
                     /gene="pfkA"
                     /locus_tag="PC414_000890"
     CDS             complement(40114..41073)
                     /gene="pfkA"
                     /locus_tag="PC414_000890"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDF2463299.1"
                     /translation="MKTIGLLTSGGDAPGMNAAIRAVVRSAIYYGCKVYGINRGYKGL
                     LEEDLTEMNLSSVGDIIHRGGTILKSSRCEEFKTEEGRLKAVKILKKYKIDCLVVIGG
                     DGSFAGAQKLSDLGFPAIGIPGTIDNDLAYTDYTIGFDTAMNTIIDAIGKIRDTSSSH
                     ERVNIVEVMGRHCGDLALYAGLAGGAETIIVPEVEITVDEVALRLKTTQKRGKRHSII
                     VLAEGVGSASDLEKELKKESGADLRVTVLGHVQRGGSPTVSDRILASRLGVRAVELLL
                     DGKSARVVGIKENKIIDLEISEALAQKKVFDKEAYEMAKILSI"
     gene            complement(41282..44851)
                     /locus_tag="PC414_000891"
     CDS             complement(41282..44851)
                     /locus_tag="PC414_000891"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDF2463300.1"
                     /translation="MNKDFAHLHVHTEYSLLDGFSRVKKLIKRAKELNMSSIAITDHG
                     CMFGVIDFYKTAIKEGIKPIIGCEVYTAARGLRDKDPNYDKYQGHLVLLAKNMEGYKN
                     LIKIVSTSYVEGFYYKPRVDMEELKKHNKGIIALSACLAGDVARALMNRNYEKAKQFA
                     LDYRDIFGEENFFLEIQDHNLPEQKEVNSGLVKLSKETGIPLVATNDVHYVNKEDSKI
                     HDVLMCIQMGKTLNDPNRMRFGSDEFYLKSREEMEELFPYALEAIDNTVKIADMCNVE
                     FDFNTIHLPKYDVPEGYTPETYLRELCFNGLKERYDNPSNEILERLNYELDVIEKMGY
                     VEYFLIVWDFINFSKENNIIVGPGRGSAAGSIVAYTLKITDIDPIKYSLLFERFLNPE
                     RISMPDIDIDFCYERREEVIDYVKRKYGDDHVAQIITFGTMGAKAAIRDVGRVLDIGY
                     NKVDKVAKEIPFALGMTIDKALDTNPNLKELYDQDPETKEIINISKQIEGMLRHASTH
                     AAGVVISKNPVDEYVPLYKHQDAITTQFTMTTLEELGLLKMDFLGLRTLTVIRDTLDL
                     IEKNRVIKNYTEKIDFSKMDYDDPKVYEMLSSGNTLGVFQLESAGMRSFMKQLKPDNF
                     EDIVAGISLFRPGPMDSIPAYIKNKSNPKDVTYLHEKLKPIMEVTYGCLVYQEQVMQV
                     VRDLGGYSYGRSDLVRRAMSKKKMDVMEEERQYFIHGKFDEDGNIEIAGCIRNGVEEE
                     IANKIFDDMIDFARYAFNKSHAAAYGVLAYETAYLKTYYPVEFMAALITSVMGNTDKV
                     VEYIRECKALEIDVLPPDINKSFSKFSVEGDNIRFGLAAVKNVGVNIINNIIAERESN
                     GLFVDLVDLVKRLDQKDTNKRVIESLIKCGAFDNISENRASLMAGYESLLESVSMDRK
                     KNVQGQISLFDAFGDSEEDSDFQQIYSLPKRQEYEERERLNLEKEVLGMYVSGHPLSQ
                     FEKELQEKTSMDNGKLNSLKEEEESYLQMNETDVIMGGMIVTKTIKTTKRNEIMAFIE
                     LEDLYGAIEIIVFPQLLQKYNVILNEDNIIYVKGTLSIKEGENAKLIAREIKDINDDT
                     DFADRNYNSGRTRNVENIKPESGKKLYLKIDSMSDTDTTNNIVEIAKQYPGNDSIYLY
                     PMDENINGKRKAYKMTGALTCICDEFINNLEELLPKDNIKVKA"
     gene            complement(45008..45955)
                     /gene="whiA"
                     /locus_tag="PC414_000892"
     CDS             complement(45008..45955)
                     /gene="whiA"
                     /locus_tag="PC414_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HDF2463301.1"
                     /translation="MSFSTETKNELARVISGNECCNIAELSALVKSGGSIQIVGYKKL
                     NLKITTELNSIARKVFKLLKKNFNINTTISVNKNQMLKRNNSYVLMVTSEMGSEMLLK
                     KLGILEDKEGFFTINKISENLIKHEGCKRAFIRGAFLGGGSISDPEKNYHMEFVTNNE
                     EFADSLKELINSLGFNSKIVARKNNHVVYIKESEQISDLLSIIGGHHALLSLQNTKIV
                     KEMRNNVNRIVNCETANLSKTVNAAVRQVENIKLIQETIGISSLPENLQEIAKIRIEY
                     EDMTLKELGEMLEPPIGKSGVNHRLRKIEEIATDLKKKS"
     gene            complement(45972..46397)
                     /locus_tag="PC414_000893"
     CDS             complement(45972..46397)
                     /locus_tag="PC414_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDF2463302.1"
                     /translation="MREEVSAGGVVLFGNTILLLRKFNGDWVLPKGKVEEGENNQEAA
                     LREVSEETGVKADILKYLGEIHYTFKENWDENRAVHKTVFWYLMQAKNMDTIPQKEEG
                     FIDAKFIHLDRVVDLARYDDEKEIIKVALQEIKKRLKKN"
     gene            complement(46466..47572)
                     /locus_tag="PC414_000894"
     CDS             complement(46466..47572)
                     /locus_tag="PC414_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HDF2463303.1"
                     /translation="MAKLLIIVGILGWIALIVSLFIYKKAKNEKVKLFQRSIVENREP
                     NVVVIGGGTGQSVFLRGLKHTTQNITAIVTVADDGGGSGVLREDLGMLPPGDIRNCLL
                     ALANIEPTMNEVMQYRFTEGLLKGQSFGNLFLAAMNGLYGNFEKAVYKLSEIFAITGR
                     VLPVTLEDVNLVAKLENGNIINGESSIPEESKIQKSSIDKIFLNPKDVKPLKDVIASI
                     YDADIIIMGPGSLYTSIIPNLLVEGIVDAIKSSVAPKVYIANIMTQPGETEGYNVLEH
                     VNAIVKHTDENLIDYVIANNEILPEEMLDLYKQDGAEQVLLDKKQKDKLKEMGIKTVE
                     KNLIEIKNNYIRHDAKYISNIVIELALNHNYNKS"
     gene            complement(47584..48441)
                     /gene="rapZ"
                     /locus_tag="PC414_000895"
     CDS             complement(47584..48441)
                     /gene="rapZ"
                     /locus_tag="PC414_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HDF2463304.1"
                     /translation="MKFVIVTGLSGSGKSETMRALEDMGFYCVDNLPPALITKFAELC
                     YQPNSSIDKVALGIDIRGRKFFEALHESLNYLEKENYEYEMVYLDCNDDVLLKRYKMT
                     RRNHPLAKDMQIPEGIKMERKIMEPLKELSTCIIDTTNMKPKDLKEEIKKIYSSGEDN
                     PNLTISVVSFGFKHGILADADLVFDVRFLPNPYYVEELRAKTGDDKEVRDYVMNSKIS
                     EEFYVKLLDMIHFLVPQYIEEGKQHLVIGVGCTGGRHRSVTITNLIAEDLSNKGYRVV
                     KKHRDSMLR"
     gene            complement(48472..49185)
                     /locus_tag="PC414_000896"
     CDS             complement(48472..49185)
                     /locus_tag="PC414_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HDF2463305.1"
                     /translation="MKILADYHTHTLYSHGKGTIEDNVKVAISKGIKTIGISDHSYKH
                     IAYGVKKKDILKMREEVDYLNDKYTDINILLGMECNILDDKGNIDMDDKFIRDFDYIM
                     AGYHFGSTPTSLRSLLNHCNNYIFKTEKAKEYNTKAVINAISNNDIFIITHPGDKGDV
                     YIEEVAKTAKKTNTRLEINSSHSFLNVNQLNKIKGLENKFIIGSDAHCPERVGDFELA
                     MKTISDSNLDISLVENIII"
     gene            complement(49189..50103)
                     /gene="murB"
                     /locus_tag="PC414_000897"
     CDS             complement(49189..50103)
                     /gene="murB"
                     /locus_tag="PC414_000897"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HDF2463306.1"
                     /translation="MNNQDIYINLLNILSKEDIKVDEPMKKHISFRVGGPADILVRPR
                     TEEQLKNVLKLVKKESIPYLIIGNGSNILIKDGGIRGVVIELADNFNSYEINDTRMTA
                     QSGALLSVLGKALQKQELKGFEFASGIPGTLGGALAMNAGAYGGEMKDIVKSVRLMDM
                     EGNIFELSNEQMEFGYRKSIISKNGYIALSAELELQEGNYDEIKSLMDDLATRRITKQ
                     PLNFASAGSTFKRPTGYFAGKLIEETGLRGLTLRGAQVSEKHCGFVVNQGEASAKDIL
                     DLIYVIKSAVYAKFGVMLEEEVKILGED"
     gene            complement(50376..51146)
                     /locus_tag="PC414_000898"
     CDS             complement(50376..51146)
                     /locus_tag="PC414_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDF2463307.1"
                     /translation="MSKGFLTPGETAQATISAGIKKANISTQNAILLGLFGGAFIGFG
                     ALGSMIVSQTFGKIDPGVASFLSAMVFPVGLMLVVVAGAELFTGNTLMAIALMDKKIT
                     LGKMLRNWGLVYFANLVGSILLVALVFYSSTLTGDAATKAIAVAEAKVNSTIASMFLK
                     AILCNILVVLAVWMATASQDIMSKLASCWTVIMLFVLCGFQHSVANMFFIPMGMALGA
                     NITMVQFITNLVFVTLGNIVGGSIVVGGIYYLCYVKNS"
BASE COUNT        17087 a         8726 c         5787 g        19647 t
ORIGIN      
        1 tataaaaatt tatttaacaa tttattattg taatattttg aatatataat aaaattaata
       61 ataagaaaac tttttataaa tatactttta attgtttaaa ttggaggaaa aaattatgga
      121 tgaaaagata acaaaaaata attataatgt tccatttgac tatgttcgaa aaaaactcaa
      181 agaattagac cctcatacaa ttagtaattt aagtaatata cctttcaata atgatttaaa
      241 tagattcaca ctcaattttt ttgatagaca atatattata gactatccaa gtggagaagt
      301 ttggaatagt aatggtagcc tttgcaataa ctttgagctt aaaatcttga tagttagata
      361 tttaatcaat gctaaaggta ttcctagaac taataaatat gtaactttta aagaaatacc
      421 aggaggcaat gtttattatc caaatttttt aaacaggact ctatataggc tttcagaagt
      481 ttttacatct caaatagaca aatataagtt agttatggag aaaattggag ctgaaaaagt
      541 aaacatggga gatttagctt ataggtttac atatatgggt aatacatcta tggtatttat
      601 actttggttt ggagatgatg aatttcctcc aaattcaaat atattatttg attctaatat
      661 agtttattac ttcaatgcag aagatttagc agttgttcct gatacagcta taaatgcaat
      721 tttaaataaa ataaatcaat tataaatata aataatatta taataaagtt tttgatagat
      781 ttaatagaat ataatatgaa atggagtaaa aaatgaatgt agaaaatcaa tctttatcag
      841 atattgaaat aatattatca cacaggtatg ataatggtgc agactattgg actacacctg
      901 ataagcgttt aataaaaggt tctcctttct ctactcttga tagtgtactt tatctattag
      961 aattaggtgt gaaccctgat gatactttac ttaaagaatg tgctgacttg atatttagca
     1021 catggcgaga agatggacgt ttcaaactat atccaaatgg aggtatttac ccttgtcata
     1081 ctgcaaatgc tgctaacgta ctctgtcata tgggatatgc atctgacatt agaatgcaaa
     1141 aaacgtttca acatttttta gatacacagt atactgatgg tgggtggcgt tgcaataaat
     1201 ttagttttgg tcgaggtcca gaaactgaat actcaaatcc actaccaact cttaatatat
     1261 taaatgcatt ccgttttagt aattatctaa ataaagaatc aaaacttgac aaagctgtag
     1321 attttctact tgaccattgg actattaaaa aaccaatcgg cccttgtcac tatggaattg
     1381 gaaccttatt tatgcaagtt gaatatcctt tccgtaatta taacctattc ctttatgtct
     1441 acgttttatc attttataat tgtgcaaaaa aggataatag atttttagaa gcattgaaga
     1501 tacttgaatc taaaatgata ggtaataaaa ttgtcgttga acgtgtagtt ccaaaactat
     1561 ctaaattatc tttttgtaaa aaaggcgagt caagtgaaat agccacaaca cactattacg
     1621 aaatattaaa aaacctagaa aaataatagt tatatagata aaacatatta aaaaatagtg
     1681 gctttaaatt ttttaaagtc actattctat ataaatataa cattataaaa aatatattaa
     1741 actatttact cttttttaaa accgttgatt ttcttgctat ataagaaact attattatag
     1801 aaataactac tagagaacct ctatttataa agaaataatt ctttaattca ttaaatctaa
     1861 catctatatt gttaggcata taaaacatct tttgaatata aaatccattt acattaatca
     1921 tactaacaaa tgggtacata tgtaaaataa aaaatatatc agcaaatgta tatgtaatta
     1981 atattgctat tgttgtaaat ctattttttg ttaatgttaa tatcattagt ggaaatgttg
     2041 tataccataa taatgtagga aatgtcttta aaaacacatc tattggcttt acactataaa
     2101 caaaatctat tttgtctatg ttaaaacctt tcactgacgt aaatgtatat actattgtaa
     2161 ttgaaattaa taataataca gtatatatag ctaatataac acaccatcta aatactataa
     2221 attgattata ttttttttta ttatataaag taagtatctc atttatatta cccgaataat
     2281 cttttgaaaa cacataaatt attgtgattg ctgacattat tggtataaaa acatctgata
     2341 tctttataat tcctaaccat cctgctatag aatatgtatc cattataggt acatcattat
     2401 aaaatattaa ctcattataa tctaatatgc ctgtaataaa tatgcataag atagaaataa
     2461 ttacaaatat taacacatgt ttaagctcta tttttttaag ctcaaatttt attaactcca
     2521 atatacacat ttattttaac tccttatgta aaaagtttat ataagcatct tgtaaagttg
     2581 tatctatcct taagctatct ttaagtttct ctgtattttt acctatatac tttacttgca
     2641 caccatcttt aactctccta taatcaataa tatcaatatt acgttctaaa tattcaaatt
     2701 catcattaga aactactacc gaatacacct caaccatatc aataaactta ttaattgtac
     2761 catcatataa tattttgcct tgatttaata taattaattt gtcacagtta aattgtatgt
     2821 cttctactat gtgagttgaa attaatacta tagaattttt acttatttct ggtaacaatc
     2881 ttctaaattc attcctttgc tctggatcaa gaccaactgt aggttcgtcc ataactatta
     2941 acttagaatc acctattaaa gcctgtgcta tacctagtcg tctctttaca ccacctgagt
     3001 atgtacctat ttttttattt ataaacttgt ccagattaac ttttgatact actttttgta
     3061 tatgtttctt taaatcattc ttagatacac ctctcatttt agctacaaat tctaagtatt
     3121 cttgcccatt tatattatta tacatatcaa aatcttgagg tacatatcct acatttgcct
     3181 tgattttctc attgtttttg ctatacttta tattatttaa ttctatctct ccaattgaag
     3241 tttcataaag agtcactaat aacctcatta atgttgattt tccagcacca ttaggaccta
     3301 ataatgcaac tactccatct attagggtta aatttatatt atccaaagct tttttatttt
     3361 tgtaattaaa atttagattt cttatttcta acttgtttat catattaacc tcctttatta
     3421 tttatctatt ataaggatat ttcctgaaag ccaatgataa atagttatgc taattttctc
     3481 tccctttgag tcttcatata aaaattcatt gctaggtaag tcagcaacta atatcatatt
     3541 aatttttttt ctttttattg attccataac attatctact tcatctacat attttttagc
     3601 atctacagat gctttgctat cacctaatat ttcatttctt gtttcaatta aaaatttaat
     3661 tttttcctct atatccatat ttacattact atctgatgga tagattattt ttatatcatc
     3721 ttttacctct tctttaaact cacctgcata tattgcaata tcatttgact ttccttcaaa
     3781 tatataatct ttttttaaaa aattactagt cttattcagt aaaggtaaat ttgaatagac
     3841 attattttta gaacttaaat ttattttata agccatacaa cttgattgat ttacttttgg
     3901 gtaccacgct aaagaacctg gtggaagcat aatatcatct ttagtagcta tatatttatt
     3961 ttgattccaa gatgtcaaca cattaatata gccttcatac tctatatcta atgtcacctc
     4021 gtcattaggc tctaaacttt cctttaaatt aacttcaatt aaatcctttt ttttgttaaa
     4081 ttttatattt tccgaattta tttttatata ttttatatca aatatttcat ctaaaatcat
     4141 attaatattt gttaactctt catttgatgt gtttaataaa acaattctat cttttatatt
     4201 tgacttgctt ttaaagtcaa tcttcatatc atgcgatttt attttaatat cactaatatt
     4261 agatttcttt aaatactctc cataactacg ctcctcatct ctatatgttg acatatctat
     4321 tgatatataa tttaatccaa taaataaaat actgtacact actataacta ttaatgattt
     4381 aatctgtttc tttctaatat catttaatat cagatatgct actattatta aagtaattgc
     4441 aaatagtgta ggtataatct tatctataac atatgctgaa ttattaactt tccctacaaa
     4501 atcagaatag taagcatatg attgatcttc aaatatgtta agtgatttaa ataatgcttt
     4561 aaaaaatggt tctttaatat caataactct atccaacata ttagttgatg ttaaatataa
     4621 aaatactgat gataaggtaa tctttagtat taaattattt ataataactg aaataaatga
     4681 tatcactata gataaaatag taagtggtaa aaaccattca aatatcaata taaatatagt
     4741 tgatttaatc gttacaattg acaatgttct tattaaactt gagtatgcta tagcatatat
     4801 aattggtatg caacaaaata taaagttgtt tctagttaca gcaacagaaa tacctaaaaa
     4861 ataatcaaaa ttatttctac ataatatttt tacagtatca taactttttc tcatataaaa
     4921 aattacattt aacatggtta aaatactaat tgtcatacaa gtatatacac tcaaggataa
     4981 gtaatcatta aatgaaattc ctctattgga tatatgtgtc catcgttgta tgagatggta
     5041 atttataata ataattgtta ttgcaaataa aatatttttt ctaattattt tggttataaa
     5101 gtctgatata gccttaatac tactcatatt tatgcccctc ctagccagtc ttttgataca
     5161 acctacaata gattcaacta tatttaataa gttcaaatac ttaccttgac attatttttt
     5221 taaagttaat attaattagt gaagttaatt aatttagaaa atgttttata atcttactga
     5281 tcactattaa ccttcattcc aatatatatt aatatagcaa tccaatatat agaaattatt
     5341 gcataaatca catattgctg aaaaatatta agtattttac ataaaataat taaacatatt
     5401 attacgaata gtattttttg cacagtcttt ctataatgct ttttctcact ttcactaaga
     5461 ggtttgtttc tatgttctaa aggagctaac ttgtaaattg ttagtatact aataatcatt
     5521 aataatacta ataaatactt atatttattt atatctatat tattagctga aaaaatagtt
     5581 atcaaataaa taactgcaaa tgttaatgta cactctttat aattcctagc atgataacct
     5641 cctgtgaact gtcttaaagt gcaataacaa cttaaaaata taactgtatg tataaatcta
     5701 tcaaaaataa ttcctataaa taatgctacc aaaatattaa ctatgaaata aatcaaagtt
     5761 tcaaatccat atctatatat ttcaaaatct tcactttcta caatctcatt attaactaat
     5821 atattggcaa atttatatga taatctttta aacatatttc ctcctcaaca tgtaaaacct
     5881 ctcctttaat aagaagaggt ccttttatta atgttatttt tttagttttt gtaaagcttg
     5941 tggctctttt gcctgatgtg ctacccaaga acttgcactg tttgctgata atacagctat
     6001 cccaaaactt aatgctgaga tgtttttaag taagtttaac gctatttttt tcattttgta
     6061 ctctcctaac tttttattgg tatcattatt tttaatataa actcattatc tgagtaatta
     6121 actattgttt caccaccata tttatttact atatatttaa tactagcaag tcctattcca
     6181 tgtatcttat catcatcctt actagtttga atacgtttat caataagttt aatatcattt
     6241 accttagtat ttataaattt tattattgca aatttattta tatatgtagc tttaacttct
     6301 attctttttt ctatctcatt atttatatcc atacatgctt caattgcatt atctagtgaa
     6361 tttgcaaata ttgaacatat atcattattt ttaataaagt ttaatttaga tatatttatg
     6421 ttatcttcaa tttgaatatc atacttttta caaatactta ttttttctgc taaaattaaa
     6481 tctaatgttt tatttccagt atttttaaaa tgttcaaatt cacttatctg aaactctaag
     6541 ttatttatat atgatataat ttcttcttta gtgtcataat tttttataca aatcatatgg
     6601 tttttcaagt catgataaac atattttaat ctatcatgaa cttcgtttat attttcataa
     6661 ttcctgtagt ttgtttttat tctttcattt ataagttcat actctagatt taatttatcg
     6721 tcttgtacaa ttttccctat tactattaaa agtattatac ttgatgataa aatcaataaa
     6781 attgcaaata taaatataaa taagttttct gtagtaattg tattgactct taaattatat
     6841 tcaaatataa gtaataagct aactatatta gataatatag gtattcctat tagtatcata
     6901 tctttaggtt taaaatctaa agataattta aaatacttaa acaatataaa acctataaat
     6961 aaaaatacct ttgacataat aatagcttgt attctaaata agttttcatg ttgtgttatg
     7021 ttaatattat ttaatttatt tataaatact actagaccta ctgcagaacc ttcagcaacc
     7081 attaatccta gccaaaacaa taaactaact attatacttt tatagatttt atcttcatat
     7141 gatgttatat aaaatgatat tcctattacc atacatagaa gaatcttaat atttggaaac
     7201 actttaacta catttatgta tgccataaat ataattataa ttattgttgt aaatatatat
     7261 cttatattag atattttcct ctttccaact aagtggatta ctaaaaaaaa tataatccaa
     7321 tctatagtac ctgaaactaa attaataata ttccaaaatc ctacacttaa atttgttagc
     7381 atactatgtc ccctaatata ttagttatcg caagcttcaa gcctttaacc ctatatttac
     7441 ttattggaat actttttcca tctactataa cagtacttcc actcattgat tctactaatt
     7501 ttaagttaac tatatagcta ttatgacatc taaaaaatcc atattcattt aatatctttt
     7561 ctattttaga tatactcatt ttagtaatat acatatcatc atgtgtataa atcaatatat
     7621 tcggtctatc tgtttctata taagtaatag aatctatttt aattcggtct acataatttt
     7681 ttacatttat agttaagtaa ttatttctct ttttcatcat ctcatttata caaggtaata
     7741 cacttttaga aattttttct ttatttattg gctttagtat atatctgtat gcttttacct
     7801 cataaccttc ttgcataaac tctacaaatg aagtaacaaa tattatttct aacttatgat
     7861 caaactctct tatcttccta gcagtatcca taccatttat tgttttcatt tgtatgtcca
     7921 taattaatat gtctaaatct ttaggatagt tatttagtaa ctcttctcct gctgaaaatt
     7981 catatatgtt ataatttata ggatatgaac ttaatatttc acttaatata tcttttattt
     8041 ttattcgata atgtagttca tcatcacata ttcctatatt aatcacaatt cttaaacatc
     8101 ctttcttata tatttttaat aaaaatatat aaatttctat tcattttaca tattttaaca
     8161 tacttctgta gtaaatagct atttagattt taattttagc tttttggaca ttaagtatta
     8221 ctttttggat ttgcaatcta ttttgatttt cattataata caatttttta atatatatta
     8281 aatatattta gggattttaa taaaatattc ctcgaaaagt ttttaatatc tagtttttgt
     8341 taaataaaaa gtatgcacct tcctttttat agtaatattt taattatcgc tataattttg
     8401 aagaatacac actatatttt aaatattaat ataaaacata ttaaaaaata gtggctttaa
     8461 attttttaaa gccactattt ttttaaatga tgttcctgaa aatatattaa gttagttatt
     8521 ctaatcaatt tgaagacttt tgcttctcct tgcctatatc taacataatt tcataattta
     8581 atttaccttt ctttaaatca actcctgagt tacttctaat aatcccacaa tccaacattt
     8641 cgtcaaatac cttcctagat aaacctagtt tctcacgtaa taatgttgat acctttaact
     8701 gacatggata tttactaata atatgaactt gtgtaaggtc attaatatta atttcttgtc
     8761 ctactatttt atactctgga ataccaactt ctgctccatt tctatgtaaa agttccatat
     8821 tcatagcata ttgcttaaca agattataat cgtttgtatg gaattgttct agtatttctg
     8881 gatttaagct ttttggatta acacgagaat atatagttga gttccatgtt gaatcacaat
     8941 ttgaacactt gtatattaac catatatcca aatatttatg ttgagcatta accctaaata
     9001 aacctgaaca aatatattca gtcttttttc cacatttttt acaataccta attacaggta
     9061 actgggagat atattccacc tcccaagtaa tttttttcat aaatgcaatt tcctttctat
     9121 aagattacta aattaataga aagttatgaa aaggtggaat ataatcaagt atttttttca
     9181 aaacaagacc tcctaatttt aataaatttt aatttcaaac atataaaaag ctactacttc
     9241 ttattagata ctttacaacc agctttgtta gctactgtat aagttattga aattacttaa
     9301 attatatcat ccacttaaaa gaaactatac ctcaaacact tttatcaaaa gaaaatgagc
     9361 cagtataatt ataaaactat actggctcat ccttagatat cttttctttt ttcttgtaat
     9421 acaattcttt ggatactttt caaagataaa taatactttt ccgataaata ttgcaaatca
     9481 tatccaactt gatagtcttc gtaaatctgc atatttctaa ttgacaactc ctcacgagta
     9541 ggtgtattac ttccccattc ttttttatta ttagattttc taggaatata gatacattca
     9601 ccatctacat attcctgtat taattcaagt aattcttctg gtaatatatg tattgctttt
     9661 ttatagctca ttttgctctc ctattaaata gtaatttttc attaggaata gcaaagggct
     9721 atttatatat taaatttcat agttgcaata gcccttgcta tgcaaaaata aagattaaat
     9781 caaacataaa atctcaaccc tccttatata tcacagcgat acatataagt ctatcaacag
     9841 tataactggt tgtcaatcaa cacaaattat ttatccattt tttaaacaca tttatttagt
     9901 ttttatatta tatttatttt gataactaaa attttttcat tataaactat ctttaaaatt
     9961 attagtcaac ttaaatccat tatcttgatg ggtttattac tgaacaagga actccaacta
    10021 tacactttcc acaaatatca catccaccta tataagtgta ttttttttca tttatcatac
    10081 acatttcata acacttattc ctattaaatc catcttcttt taaagcttct ttcacgcatt
    10141 tgttgacaca ttttttgcaa ctattattat gtttatacaa gcagtattca ttttctggtc
    10201 ttttagttgg ctctaattct aaacttgtga ttatgcttcc tattcttcca caacaccctt
    10261 tttctgtaat aagcatatta tttaaaccaa aagttccaag acctgctatg aatgctacat
    10321 gtctttgtga ccagtcactt accaattttt cactgtcaaa attatacgac ccaggaatag
    10381 catatgaatt agcccccatc ttctctagtt cttcacataa gaaatcattt aaatttgaaa
    10441 tcaacatatt agtttctatg tacgctcttg cccatatttc agaacattca aaccctccaa
    10501 tattactatt atttacactt tcatcaaaag gtataaaata tgtaattata gtttttccat
    10561 tttccagaaa atcttttggc atagcatgag atgttcctac aacacttttt agcttctgaa
    10621 acatatcatc ttcagcagat gcataagcaa acaaaagttc ttgccacttt gtagttatat
    10681 tatttgattt atgataattt tttacaaagt tgttcattga tatttcaatc ttatttttca
    10741 tagtatcacc cctagataat tctatatttc caatttaatg ttattatttt atattatata
    10801 atagtatttt ataatatgcg cactaattaa tattaaaact atgttcatca atcattctta
    10861 aaaaagctcc taatatcaat acgatattag aagccttttc ttatctaatc taatttattg
    10921 caattcctgc acttgcatat cctgctacag tattaactaa tgaaagacct gaagctgttg
    10981 cagtaaatac tagtaagaat cgagtttctg ctgttgctgc tatatttaat cctgttacaa
    11041 ttccactaga aattgaacca actgataata ttcctgtaag tggtggagct agtgtaactg
    11101 tcgctcctgg tacagctgta aatgagttat ttggtgcagt agattggtaa agtgttgctg
    11161 taattgtaac tgttgagcca acaagtgaaa gtgctgctgt tgtactgaag tatgctgaaa
    11221 tagatgttat tgttccagct cttggcattg aaaatgcaaa gttagtcaat gttcctgctg
    11281 cgtttgtaag gtctattact ccaccaacta tacttaatcc tggagctgaa ctaccaaagc
    11341 caacaaaacc tggtgtacct actaatcctc cagctatagt tgtaagtgat agtggtatac
    11401 ctgatgcaaa aggtattatt gcactagcac ctgttggacc tgttgctcct gttgctcctg
    11461 ttgctcctgt tggacctgtt gctccggtcg ctccggttgc tccggttgct cctgttggac
    11521 ctactaatcc atctgcccct gttggacctg ttgctcctgt tgcccctgtt gctccatctg
    11581 ctcctgttgg acctgttgct cctattgggc ctgttgctcc tgttgctcct gctaatcctg
    11641 ttgctcctgt tgctcctgtt ggacctacta atccgtctgc tcctgttgga cctgttgccc
    11701 ctgttggacc tgttgctcca tctgctcctg tatttcctgt tgctcctgtt gctcctgttg
    11761 gacctgctac tccatctgct cctgttgctc ccgtcgctcc tgttggacct actaatccat
    11821 ctgcccctgt tggacctgtc gctcctgttg ctcctgttgc tcctgttatt cctactcctg
    11881 ttgcccctgt tggacctgtt gggcctacta atccatctgc tcctgttgct cctgttgctc
    11941 ctgttggacc tactaatcca tctgctcctg ttggacctgt tgctcctgtt gcccctgtcg
    12001 ctccggttgc tccatctgct ccggttgctc ctgttgcccc ggttggtcct attagtcctg
    12061 ctgctcctgt tgcccctgtt actcctgttg ggcctgttgc tcctgttgct cccgttgctc
    12121 cggttggacc tattaaccct gttgctcctg ttattcctat tcctgttgct cctgttggac
    12181 ctgttgggcc tgtcactcca aatcctgttg ctcctgttgc ccctgttgga cctgttactc
    12241 catctgctcc tgttgcccct gttgcccctg ttggacctgt tactcctgtt gctccgtctg
    12301 ctcctgttgc tcctgttgga cctgttggac ctgttattcc tattcctgtt gctcctactg
    12361 ctcctgttgg gcctgtcgct ccaggtgttc ctgttgctcc tgttggacct attaatcctg
    12421 ttgctcctgt tactcctact cctgttgctc ctgttgggcc tgttggacct gttactccaa
    12481 atcctgttgc ccctgttgct cctgttggac ctgttattcc atctgctcct gttgctcccg
    12541 ttggacctgt tggacctgtt actccatctg ctcctgttgc tcctgtcgct cctgttgggc
    12601 ctgttggacc tgttactcca aatcctatag ctcctgtcgc tcctgttggg cctgttgccc
    12661 ctgttgctcc tgttggacct cttaatcctg ttgctcctgt attccctgta tttcctgttg
    12721 gaccagtcgg accggttggt cctgttgggc ctgttgcccc tacacctgga cctgttggac
    12781 ccgttggacc tgttgggccg gttctacctc ttggacccat tgggcctgtt ggacctacac
    12841 atgatggtgg acagcaatga tggcaatcac agctattata atcatcacga ccattgttac
    12901 aatcatcata tttatcatag ccattgttgt aatcattatc atcaaatggt ccaaaatatt
    12961 tatttctact cattattaaa agcacctcct gatatatttg agcttttaag ctctatatat
    13021 aatatgtcaa atttagtatg attgtgaccc atcaactatt tttaaattta ctaaaataat
    13081 tttcattgtg caaaacagca ctatcattag gtttgatttc ttttgttttt tcatgataat
    13141 aaatagcttt atctgattct cctaacctgt cataacagac acttaattgt atagatggta
    13201 tatatccata acaatcatct gacttaaagc ctccatcagt atcattctta tctcttgtta
    13261 atgctacctt ataccaaaaa atggcttctt tatatttttg tctatcaaac atatgctttc
    13321 ctatgtcaca acaaatttct gctcttggct catcaaattc gaaacttcta aacaatgaat
    13381 ataaggcact tttttcatca ttaattaagt aataacatgt ggctaaatct ctgcaagcac
    13441 tgatacaatc ctcaatccat cctcttgatg agtctaaaaa cttagtaaaa ccttctattg
    13501 cttctttata cctagcatta tagtacaact ctctagaata atagaattgg tgtcttggct
    13561 ccaatgtttt tccttctgaa atcatttttt caaaaattct aagatttctt agagggtcct
    13621 gtctatgaag ctttttatga gatattgcaa tgtcagaata aaatacctct ccaaaaagag
    13681 gtactacttc atgaataggg tcaacccatt tatgattaga acttctttta aacaatcgtt
    13741 ctctataata tgagagagtt gggttaccat tttcatcaaa ggatacattg tattttgcca
    13801 taacaatatc aatggaagta tccaagcttt ctttaatctt taataatttt tctctatcag
    13861 cttctaaaat tatgtcatct gcatcaagcc acatagtata gtctttagaa gctttagaaa
    13921 atgaaaaatt tcttgcagca gagaaatcat ctatccattc gaaatcgtac accatgtctg
    13981 tatattttga gactatttct tttgtggaat ctgtagaacc ggtatctaca attataattt
    14041 cgtctactat atcctttaca cattctaagc atctgcctat aacttcttcc tcattcttaa
    14101 caatcatgca caaacttatt gtaatcataa tgtacaactc ctttgtcttc tatatattgc
    14161 agacataagt aatatatggt attttctctt gttttgttat taaatttata aaaacttttg
    14221 acgacagaaa gttactaaag aaccttatct tttatgtatt ttttatagta gaatttatta
    14281 aacttgaaat ttggtaataa ttgtcgaaat gataaaacca tatttaattt catacaaaaa
    14341 agagtgtccc ataaactaaa aagttcattt tgagacactc ttatttaata ttatatatat
    14401 atttgtaaaa cagacatttt aatatgatac tttgatactt tattcattcc tattcattat
    14461 ataatcaatc agcccgtact ctttagcctc tagtgcagtc ataaaattat ctctatctgt
    14521 atctctccta atatcttcta ttgatttacc tgtattttca gaaagaattt tatctaatcg
    14581 ttcttttatt ttcaatatat tatctacagc tatttttaca tctgttgctt gacctttagc
    14641 tcctcccatt ggctgatgaa tcataatctc tgaattgggt aaagcacatc tttttccatg
    14701 tgttcctcct gcaagtaaaa aagcactcat gcttgcagcc ataccaatac aaatagtcga
    14761 tacatcacat ctgacataat tcatagtatc atatatagca aatcctgatg tagcagagcc
    14821 accagggcta tttatatata taattatatc tgaatctggg tcttctgatt ctaaaaacag
    14881 taattgagct actactaaac ttgcaatagc atcattaatt tcttctccta taaatataat
    14941 tctatctttt aataattttg aatatatatc ataagacctt tccccttgac ctgtttgttc
    15001 aattacatgt ggcactaaac tcatgctact ttcacctcac aattaatatt aaagttcaga
    15061 taagaattga tttcatctct tctttgttta taattcatat ttgcataaga aatacatttt
    15121 tcactttgaa tttgatgatt tagatttaaa acaaatctct gttgaatagg ataaaattca
    15181 tctttatttc tatatacttg tggagattct ttatataaat ttctaaatgc tagtgtaaaa
    15241 gattgatgtg tagaatatcc tactgtaata gctatgtcta taatatattt gtctgtaaat
    15301 ataagttgtc tagctgcttc agtaagtctc ctcctctgta tgtattgatg gatagtacat
    15361 cctacaacat ttgtaaacat tctgtgtaaa tggtattttg aataattagc aatataagct
    15421 attttatcta aatttagatt ttctgtaaga ttttcttcta tataatcaat tatctccata
    15481 ataacatctg tatttgtttt caactctatc ctctcctttt aagtattata acctatgcaa
    15541 aaagtttttt cttaatataa attgctatct taatttagtg tatagcaata aagtatcgat
    15601 aaaaaaatat caggtatttt tatacaaaaa tattaatata tatttctttt aaagtttaaa
    15661 cttaattaca tctattatat gtagattgct acatgtttta tctatgtacc ttgattcata
    15721 tgtattattt tgctgagtat ttttacacct ttttcaattt catcaaaact aagatgagca
    15781 taacctagta taatttcata gtcatattct aatatattct caattgcata ttgttgtata
    15841 ggataaactt ttatacctgc ttcaaaaatt ttatgaatta aactttccgt aaatttaaca
    15901 ttatagaaat gagcaactat atgaagccct gctgcttcac cttttatttt aaattcattt
    15961 ttaaattccg tgtttaaaac ttttatgagg tacttacgtt ttctattgta caacttcttc
    16021 attctaaata tgtacttctc taaacctcca tttccaataa attcagctaa tacatgttgc
    16081 aatattgttt ctgtatgaac atcagagtac atttttaagg ttttatagct ttctaaaata
    16141 ttgtctggta ataacataaa tccaacacga atagctggag ataatatttt actaaaagaa
    16201 cctatgtaaa ttactttatt tggactaagt tcataaagag aactaattgg ttgaccctca
    16261 tatctaaatt cactatcata atcatcttca attatatagt aatcattctc tgttgcacat
    16321 ttaataagct ctagtcttct ttgaataggt aaaatccctc ctaatgggta ttgatgagaa
    16381 ggtgtagtat aaacaaaaga aatccccttt attgatttta ttaagttggt attcattccc
    16441 ttatcatcta ctttaattcc aacaactgaa tatccaatag atgaaataac ctttaataag
    16501 cctttatgaa tagggtcttc tacagcaacc tttttattac ttttgtataa aagctgtgaa
    16561 attaaagata aaccttgtgt tgaaccagaa acaatcataa tgtttttggg attacatcta
    16621 attcctctag tacgaaataa atacttagat atttccactc ttaaaaccca tacaccagct
    16681 gtacttccat atctgaaatc cgaatctgat aaatcataca gtatttgttt atatagcttt
    16741 gaaagttctt tgcgtggaaa tagttttaat tctggaactc ctgaacgaaa atctatataa
    16801 tttttttgtt tattagtatg taaatctttt tcatctctaa aagtatcatt taacttaaaa
    16861 tcaactttat gccctgatat tcctttagct acaattgttc catatccatt ccttgtttcc
    16921 aaatatcctt ctgcaattaa ttgattatat gcatctaaaa ctgtattacg agatacagat
    16981 agttcttttg ataaagtcct agatgatggc agtttttgct caaactttaa gcttccatcc
    17041 agaatcatat cttttatctc acaataaatt tgtcttgata atgatatatt actatttcta
    17101 ttaatcgtaa tccacattct aatacctcca aaagtggttc tataaaatta ctataaaaat
    17161 ggaactacaa aatccacttt taatttgcta agattatatc ataaaatata aaataaaaag
    17221 gagtctatat tatgttagaa atatcacata aagttaaaga aatttatccc aatattaaat
    17281 ttggagttat ggttataaat attacacatt caaaacctaa taaagaaaat tttttagttc
    17341 ttaaaaactc tacaattaaa aatattatag aacaacaccc agaatacaat cgtaaagaaa
    17401 aaattaaaac agagcctgtt tcaagttata taaagtatta taaaaaattc aagaaaactt
    17461 accctgtatt gcttcaatta gaatctattc tattaaaatc aaaaggtatc ccagatgtag
    17521 gtgtaacaat tgagtcaatg tttttagcag aattgaaaaa tcttcttcta acagcaggtc
    17581 atgaccttga taaaatggaa ttacctttga aaatagaatt agcaaatgga agtgaacatt
    17641 tttatggtat aggtggtaaa gaacatattt taacaaaaga tgatttattt ttatcagata
    17701 atattggtat attatctagt attcttaatg gccctgataa tcgaacagct ataacaaaag
    17761 atactaaaaa tattatgtat tttgtatatg gacctgataa aatatcagaa aagcaaattc
    17821 aagaccattt aaatgacata aaacactata tttcaagtgc ttttccagat ttaaaagtta
    17881 attcaattga tatattttag ttaatatatt aaaatcctca taaatatttt gaataaataa
    17941 attagtgaag tatacattct agttaaacta gatatacttc actaaaatta attatatgta
    18001 gatattattt attttgaatt ttgtttataa taaacacttt attacattta tcctatgaat
    18061 aacttaattt tataattaaa gagtttcata agaaacaatt tcacttaaag gaacacgagt
    18121 tttttcatgt ctctctgggc tctctggtat cttagattca tatcccatca ataatatatt
    18181 tattggttct agattatctg gcaaactaaa ctcttctcta attatatctg gattaaaata
    18241 gcacacccaa acacttgcca atccaagttc agttgcctgt aacatcatat ggtctgtaac
    18301 tatacttgtg tcaatatctg taagttgttt cccatcaaaa ggtcttgtcc aaactttgtc
    18361 tttatctcca catacaagaa ttgctaatgg agcatcatat atattggcag ccttagaaag
    18421 tttatttata ccttcttttt cctgaataac aatcaatctt tgaggttgac gattaccacc
    18481 agttggagct attcttgcaa catctaaaac cttctcaagt ttttcttttt caacctttct
    18541 atcttgataa ttacggcaag aatatctttt ttttgcaagt tcaacgaaat tcatttattt
    18601 acctcctatt gttttattag gctaagttga tgatttattt aacctatgct ataattttat
    18661 cactatggta ctatattaca ataatgcact tttttgtaat atagtaccta aaaagatact
    18721 ttaaaataaa taaaggagcc caaaaatgaa aaaatgttta gataattaca gttgcccaat
    18781 agaagcaaca cttgcattga ttggtggtaa atataagaca cttatacttt ggcatcttaa
    18841 agacactata ttgaggttca atgaattaaa aaaattaatt cctaaagcaa cgcctaagat
    18901 gttaactcaa caattacgtg aattagaatc agatggttta attataagag tggtttatcc
    18961 tgttgttcct ccaaaagtag aatactcgct atctgatttt ggaaagagta ttattccaat
    19021 tttagattcc atgtgtgact ggggttctga ttacttagaa aatctataaa ccattacagt
    19081 ttaaatccca ttgctaattt aacttcatcc atttgttctt tagatgtcaa catttctaaa
    19141 agcttaaaag ctacatgtgg agctgtttct ggacaatatg aggttataat atttttatct
    19201 acaacaattg gttcattaac tacatttaca tcaaactcac ttaactgtct ttgtctttta
    19261 ccatttttca aatgatatgt agtagcttta cgatttttta aaacaccact ttttccaact
    19321 ggcaatgctg ccacacaaat agaagcgatt attttttctt ttgaattaaa ctcccttatt
    19381 agatttaaaa aagatgaatc atatgcttca tcataaaatc caaattcttc aaatccccca
    19441 ggaatagcta gagcatcata atcatcaaca catacttctt ctatcgtctt atcaactaat
    19501 acttgtatat taaatgtgct cataacttgt tttttaaatc cacaagtcac tacatcaata
    19561 tcatgcccat aatcgttacg agcccatccc ataacatcta caaatacact aaattccata
    19621 gtttcaaaac cttttgctaa aaaaactaaa actttcatat tctattttcc tcctaaaata
    19681 taattaacac tttactagta aattatttaa aaaatatctt ttattgtatt aaattcttct
    19741 ttaattgtag ataaaacaat ggttgtatta gctttttgaa tccccccaat ttccttcaaa
    19801 gtcactgata taaactcttc aagattactt gtatcttctg ctaatacttt tattaagtaa
    19861 tcatactctc ctgaaatatg atgacattct aatacagtat catattccaa tatagttttc
    19921 ttaaattctt tcatactttt agataaatca gtattgataa atataaaagc caaaagctta
    19981 tattttgttt tttcccgatt aatttgaata gtgtactttt gaattaaatt tgactgctca
    20041 attttcctta tacgctctga gactgctggt actgataaat taatttgtct gcttatttca
    20101 gaggttgaag ccctactatt cttttttaga gaattaatta tttttaaatc tatttcatcc
    20161 agagctactc acctccttaa ttatttaata caattttagc taataattta cattaagtaa
    20221 ataaatttaa tcatttcaat aaaatttaat acaaatatgt tatttttcct taaaatttta
    20281 gttttcttta atcaaaacac atataatact taaaattatt taagttgaat taatgttttt
    20341 attttgtata atatattaga accttaatat atcattgaaa attcaacaat aatagacata
    20401 tacttctaca tgttgtaata tttatgaaaa tagtatccct atatcaaaat tataaaactc
    20461 ataatcttaa ttttcttata gactataaaa ttttattgat agcgagtctt caaataactt
    20521 tgtaatgata gatttactaa attaatataa taatagtaaa tataactttc ttattgacta
    20581 taactaagta ttatgataac ataacttaag ctataattaa taaaacatat tttaaataaa
    20641 taagataatc cacaaagtac aaaagtacaa tttaaataca gataaactag aattaatact
    20701 aagattagga tgtgaataat tatgatttat atagaattag ttaaacttgt tagaaaactt
    20761 aactctacac aaatgtatac gatgaagcgt tgtgtagagg ttaatctgaa agcttaaagc
    20821 tttctatctt catcggcaaa tataggttta gtatagtttt ttggctatgc ttatttaaaa
    20881 ttacctattt gccgatagat aatatagatt ttctggtcat agacaacaat gcttgtctat
    20941 gaccttttgt ttttccaaaa gcagaggtcg gcattttaca atgccattcc tctgcttttt
    21001 ttatttccat aaatttttta ataaaagaaa aggagtttga attatgagtt tattaaaagt
    21061 aactaattta tcacaatgtt ttatggataa atcactgtat gagaaagcta actttgattt
    21121 attcaaaggt gaacatatag gtgtagttgg ccaaaatggt acaggtaaaa gtacattaat
    21181 taaaatttta ttgggagaag ttgttccaga ttcaggagaa atcaagtggc aacctaatat
    21241 caatataggc catttagacc aatatgcaga aatcaataga gatacaacaa tatcactcta
    21301 tttacacact gcatttgaag aactttataa aatagaaaag gaaatgaatt tactctatca
    21361 aaaaagtgct atatctgaaa atgagcaata tctaatcaaa gcatcagatt atcaggaaca
    21421 actaatagca agtaatttct attctataga taatgaaata aataaaatag caaatggtct
    21481 tggattagat tcaataggaa tgaatcgtgt tgttggagag cttagtggtg gtcaaagggc
    21541 aaaggtaatt ttagcaaaat tactactttc taaccatgat gttctattat tagatgaacc
    21601 aacaaacttt ttagataaag aacatgttga atggctctca aattatttaa atacatttaa
    21661 tggggcattt attgtagttt cccacgattt tgacttttta gaaaaaattt ctacaggtat
    21721 tcttgatata gaatttggca tgattaagaa atatcatgga aaatattcag agttccttaa
    21781 acaaaaatca catttaagag aagattatat tagaaggtat caagcacaac aaaagaaaat
    21841 agaaaaagaa gaaacattta tacgtaaaaa taaagctgga gtaaattcaa aaattgcaag
    21901 aggaagacaa aaacaattgg ataaaataga aagaatagct cctcctagtt ttacaggaaa
    21961 accaaatatt caattttcag aaattgaaat atcggctcaa aatgctctta ctataacaaa
    22021 tcttgaagtt ggatactact attctttact tccaaaatta aatttctctg ttgatggagg
    22081 tcaaaaaata gttataactg gttttaatgg aatcggtaaa tctaccttgt taaagacatt
    22141 agtcaaagat attcctcgta tatctggaga tttccagttt tcagaacaag ttaaaattgg
    22201 ttattatgag caagatttaa aatgggaaaa tcctgataaa accccacttc aaatagtagc
    22261 agataaatat ccaaaattaa atacaaaaga gattagacgt catctagcca gatgtggtgt
    22321 gaaggaagag catgtttcaa ggagtgtatc tactttaagt ggtggtgaac aatctaaggt
    22381 taaattatgt tgtatgatgt tatctccttg taattttctt atcctggatg aaccaaccaa
    22441 tcactttgat gcagagacaa aggatgcttt acaaaatgct ttgaaacagt ttagaggaag
    22501 tataatccta gtttcccatg aagaaaaatt ttataaaggt tggattgata aagtatttaa
    22561 tatagaaaaa caattagtat aagtttatac acaatataca atattattta attagtattt
    22621 caatgcaaat tttaatgttc tagttaatgt ttcactgcaa ataaactaaa aaaattttat
    22681 tagaagataa agtacattac caggatataa acttaatgag gctatctcaa aatagagata
    22741 gacctcttct tcattgttga gataacgaaa atatatttga ttttacccat aaaagaatgc
    22801 ctcattaact aaaaattcaa cttgagatat cttcattaat attaaaatct attctaatcg
    22861 attctattca caagtttata tcctagtcct ttaactgtta taagtatttt aggatttgag
    22921 gggttatctt caattttttc tcttatatgt cgtatatgca ccataattgt attgtcacaa
    22981 atactactat atactcccca tacttgttca tagagtcttt cttttcctat aattttattt
    23041 acattttgag ccatgtagct taaaagatga tactcccttc cagttaactc aatctcattt
    23101 ctatctttat atacacggtt tccttctata tcaatagtaa tatctcctat tttaataact
    23161 tcactatctg atggtacaat gctttgatac tgttgtctac gtaattgcgc tttaactcga
    23221 aaagcaattt ctcttggact gaatggcttt gttatatagt catctccccc actactaaga
    23281 cctagtattt tgtcaatatc atcatttttt gatgataaaa aaagaataga acagtatgaa
    23341 aactctcgaa tttttttaca cacttctata ccatctatat ctggtaacat tatatcaaga
    23401 atgacaacat ctggacatgc tatcttacac agcgaaatag catctagacc attataagct
    23461 ttaataatgt ttttaaaacc atcatttatg agtacttctt caattaaatc tactatatcc
    23521 ttttcatcat caacaagtaa tattttttta ttaaaacatg tatccatatt gaaatctctc
    23581 cataaaaatt tattatattc taatcttgca gtatcactat cttgtttttt atattcacta
    23641 ctcttgcttt tacattacta atagtaacat ctctaataga caaattcaat cactatgaaa
    23701 attaagataa gtttctttaa aataaagtgt atgatacatt aaaacctata gaaaattaat
    23761 ttcatccaaa gtttataata ttagactaat acacttacag atacagatta atacaatagt
    23821 aaaataaaag gagttgtctt aaaattgact ttttctaatc aaactttaag ttcaactccc
    23881 ttttaaactt ttatatgtta tttttattaa ttctttatat caattataac tttaattttc
    23941 aatagtatat atctcccact aattgaatta cttattaaaa cataaaatat ctacctaaac
    24001 tatttattta atatctacat atatttatat ctattcttat acttacatat acaaaaaatg
    24061 gttaaaaata aagttaaaat ctcagcactt ggtactgcta accatatccc atttatattt
    24121 aatatatttg gcaatatcaa taaacctata gttataaatc caaatgtacg tagaaaagat
    24181 aatattgcag acacctttcc atttgataat gctgtaaaca tagacgaagt aaaaatatta
    24241 tacccacaaa aaataaaact aaaagaaaat atataaaatc cagaccttgc aatttcataa
    24301 actcggcttc ctctttgaga aaaaatctct acaatgattg gtgaacatga tatagaaaag
    24361 ataaatacca ataaagatgc tactgaaata aataatatac aaatcttaaa aacatgattt
    24421 aactgcgtat tattttcact tccatagtta taacttatta ttggtgcaac acccattgag
    24481 aaacctatat aaagcgaagt taaaagaaat tgagaataaa taattattgt aatagctgca
    24541 accccatctt caccaattaa tttcatcatc atagcattaa ataaaaaagt agtaactgct
    24601 gttgatagtt gacccaccat ttcagatgaa ccattaaaac aagcttcaaa taatattctt
    24661 ttatctagtc taggtataca aaatttaagt aaccctttat tttttgaaaa gaaaaccata
    24721 cctgaaacgg taggaattag gtatcctact ccagttgcaa gtgctgctcc actaataccc
    24781 atttgtaaca gaacaatgaa cacatagtca agaattatat tagcacaccc agcacctaac
    24841 actagccata atccaaattg aggctttcca gctgttacaa ataaacattg aaataatact
    24901 tgtagtatat ttgcagaagc aaaaaccaag ataataaaaa gatatgaacg acaatattta
    24961 attaaaactt cacttgctcc taaccatata atgatataat ccataaatac aatacctata
    25021 ccagtaatta ttaccccaat tacaactcct gataatacga ttaaagaaaa agctttattt
    25081 gctccctcaa tatcaccatt tcccatctta cgtgctacaa tagcacttcc tccagtggca
    25141 agcatagttc caattccaac tataagatta ataattggac atacaatatt aattgctgat
    25201 agagcatttg tattaataaa tcttgataca aatatagtat ctacgattgt atacaacccc
    25261 ataaaaatca tcataaaaat agtaggaaat gcaaacttca atagagtaaa taaattaaat
    25321 ctttgtgcca gtaaattatc ttgctcataa agattatctt gttcatgatt ttctgaataa
    25381 tatacatcac tcattttgtt tatttttctg tacattaatt atttttaaaa tatattccaa
    25441 acaattagac atttacagac ctcctttctg cataagaatt atatctaatt atttggatat
    25501 agttttaatt aatcttgctt ttttacacaa ttatatttct actaaagaaa cttttaaatc
    25561 cttactttaa taattttata gctttcagtg cctttagcgc aaatacttgt tcacatagtt
    25621 tagtgtcata tgaaacgtat ttatatatag ttatacattg tctattttta ctaatttatt
    25681 aactacttgc acttttataa tgtctcatac ctaacttcca aaatctccac ataaacaatg
    25741 taaacaaaaa tacaattaaa attgagtata ttaatccatt aattgttatt gtcccagcca
    25801 aaatttgagt cggaattgtc gacataattc catatggtaa gattaaatag aataatactt
    25861 taaaaacacc ttcatatatt acaccaggaa ttttcatatt cattggcaat aaattatctt
    25921 ctaatctcat tatcgcagaa gatgatataa caaaaaatga aactgttcta agtatgatac
    25981 atacatcata aaaaagtaat gtcatcaata acaccatgaa aatatatatt attatcataa
    26041 ctaaagttat ctctgagttt aactcagata ttccatataa aacaataagt atacttgcta
    26101 gaattaatgg tgatgaacca atattaatat tttcaaatgt taacctaaat agtgggtcta
    26161 atggttttgt aatatagtag tctaattctc cttcttggat ttttcgtgga atatcataaa
    26221 taccaaaaaa gaaaatcgtc atatttatcg catttattaa tgagaaagtt cctataaata
    26281 ttaacatctc tccttgttgc catcccccta ttgagtctac atgagaatat atactattaa
    26341 acattagaag ttgtaatata aataatgaac tatctacaaa aaatgctcca aaaaaatcga
    26401 atctaaatac catcatcttt gataatttca tttttaataa cataaatatt attttcaaat
    26461 tttttttcat catataccca ccccatcaaa taatgttctc attcttttat acataacttt
    26521 atttactaca gaaaaaaata aaacccataa tgctagaata attaatcctt gtattgcctc
    26581 attgctgttt ctacctatta gtaacataga tggcaaatat gttgtataat aaaatggaaa
    26641 taccttcata actgttacta tagtttctgg taagagtaca agtggtatca ttgttcctgt
    26701 aataaattca acaatatttt caattatcat tttaaaaaaa ctaatctcaa gaaatttaaa
    26761 tgacaaaatc ccaataaaga agtggagttt catcataaaa ataaacccta gaaaaatcat
    26821 caaaattgaa attaatattt gaactggatt tgatgtaaag gcaaaatcta ttttaaatat
    26881 aaaaatccat acaaatgcag aaatcatatt aaatacaaaa taaaaacatg tcattccaaa
    26941 atttttagca ataaaataat tttgaacatt aattggaata atcatgtact ttgaaaaggt
    27001 gcccaatttt acacgattac atatctcttc actggtactc tttgacatat caatacctgc
    27061 taaaaatgag cttattatat agtaagacaa catagagtta aatgtaaaac cagagactat
    27121 tttattttca ccaaaaattg ctttccataa aatataagca aataaaattt ttacaatcgt
    27181 aaacaccata ttaaccacca catcaaatct ccagataagt tgcactttaa agttaatttt
    27241 ggctacttca taataccttc taatactctg tttaaattta taaaacattg aatcatttga
    27301 acttgcttca ttatagttgc tcataatact catcacttcc tttcttatat attctctcta
    27361 caatactacc tatatcttct tcttcaatca ttacatcttt aacatcatat ttatcaaaaa
    27421 tccatttaat cgtttcattt cctctttctt ttggcacaat tactgatatc ttatgatttg
    27481 tttgttcaac aactttacaa tcttttggaa atacaatgtt agtttcattg gaaaaactaa
    27541 ctgttatttt tttatatttt tgaaaatgat taaatagttt ctctgtagaa ccatcatata
    27601 ttttacggct attttctaca acaatacttc tactacagag actcttaata tcttccatgt
    27661 aatgagaagt aagtataata gtagttcctt ttgtttcatt tacttctttt aggaaatacc
    27721 taatttgttt ttgggctata gcatctaagc ctatagttgg ttcatccaaa aataatattt
    27781 ttggattatg tagaagggct actattagtt ccattttcat tctttctcct aaagaaagcg
    27841 tcctaacttg aacatttaaa tagtcagata cctcaaataa ttcgataaag taatccaagt
    27901 tttttttata ttcttcatta gatatatcat ataattcttt aaataaaaga aacgtatctg
    27961 ctggtgtcaa ttcaaagaat agttgacttt tttggcccat aacaacagca tattgttttt
    28021 taaaattatt ttctaatttg ttagggtaat aacccattac agatatttct ccagaagacg
    28081 gtgcaataat accagttaac atcttaataa gagtagtttt accagctcca ttttgaccta
    28141 ttaatccaac aaattctcct tcttctatct ttaaatcaaa tgacaatata gctgtttttt
    28201 ctgtatattt cctattaaat aaagacttta cactccccat aaatcctgcc tttttctcaa
    28261 atcttctata agctttagtc agttcattaa gttcaattat actcatttta actacctcca
    28321 tatttcataa tttatataaa aaaagtccac aggtatatat ctgccttttt ctgtgcttat
    28381 atttaacagc acaaaaacag aaaactcctg tggactagaa aaattaatgc tctctatttc
    28441 aaaatagtta tcaaaataat actatacaaa attaataaaa gtgtagcaga ttaaagccta
    28501 ctacaccata atttaacaaa gtaatatata ttttaaatac tatttgttgt catatgtcag
    28561 ttatctataa aggcagaata agacacaaaa attaaatgta ttaaaaatac acttgaaatt
    28621 tctgattata cagaatatat gtgtctccaa ctatttaatt gttggttacc attatataac
    28681 tccgacatca aggcactcct ctcataatat tattattgat atcatatcat aaaggttatt
    28741 tatgttcaag catggattaa tatactaata catttagctt atttcttcaa ggttgaattt
    28801 aaatttcatt tataatattc gtttataaat taaaaatata atacgtatat taaaactggt
    28861 cattttactg taatattttt attgtaaaag atatactttt acctatacag gatgtaattt
    28921 ctttagttct atattataat aatattgtaa taagagttca acttataata cattaaaata
    28981 taaactgtaa atcttaaggg ggtaatctat ggaaaataat acaatacaaa aacaattaag
    29041 agatttatca aaaaaaattt ggcatattta tattttaaat ccagaagaag aaaaccttaa
    29101 atttattatt tccttattag atgacaacct tgcattaatt ggaacaggaa aacatgaatt
    29161 ttacgaaaca ttagaagaat ttattatagg tcttgaaaaa gaccaaacag aatcagagca
    29221 aatagacttt gagattatag atgagtggta ttcatgtacc aaagtcacag acgaagcttt
    29281 tatagtatat ggtactcttt gggtaataga aaaagattac ctagaaaaag atatctatgt
    29341 agaaatggat actcgattta ctatggtata cagagttaca gatgatatga ttaaacttat
    29401 ccatgttcac cattctattc caaactttga ccaaatgaaa gatgagtatt accctaaaac
    29461 aataactgaa aaagcacaag aagcactagc aatggcaaat gaatttaaac taaaatcaga
    29521 aaaagacctt atgactggac tatacaatag acattttttt gaagtacaca taaaagaaat
    29581 gcttaaaact gtttcacatg gatactttta catctttgat ttagataatt ttaaacaggt
    29641 aaatgatatc tttggtcatg ttcgaggaga tgaaatacta atacattttt caaatatttt
    29701 gactaaagta ttcgtaaatg atgctattat tggaagactt ggtggagatg aatttgctgt
    29761 ttttgaatat agtaatcaat caaaagagaa tgttgaaaat aaggcaaaga aggttttaga
    29821 attatgccta gagagtaatt cttgttctat aggtatatct aaattagaaa ataactctga
    29881 tactttccag tctttataca aacaatcgga ttatgctttg tattattcaa aacgaaataa
    29941 taaaggaaac tttagttggt tttagtacaa aagatattaa aataggcaaa tttattagag
    30001 tatcttgtcc tatatactaa taagtaaagt aaacaacaag ctatctttgc atatatttat
    30061 ataaaccaac tattcttaat aaaactacaa tagtcaacat gaccactatt atagaatata
    30121 ataaactaaa aacatgatta atattatatt ttaataattt tatttttaaa agtaagtcca
    30181 aaatgttata tgttttgatt aaaactgatg ttgatataaa aaattttact taaaaaaaca
    30241 cttagaaagg ctttataaag agcctttttt gcttttattg attaaatttt catataaaat
    30301 tccttattaa atttcaaatc aataaaaaaa ggctctataa gaagcctccc cctattttta
    30361 ttgattaaat cctaatatag acaatgcctt agcatcatag taagtagtca tctctcttgt
    30421 tgcatccttt aatttacggt aatcaaagtc taatgatcca ccaaaagctg atatttcaga
    30481 cccatcaaac ctataccatt tcccatcaat ttgtatatag ttccaagcat gatttaatga
    30541 tggatttaat atagatagtg aatccattcc cattaacctt gttaataaat gaaagctact
    30601 tgcaaaactt ccacatacac cacgtttatc aaccagatta ccatagacta tgttacaata
    30661 tttagagtct gcatattttg cttcggttac caattttgca gctctattag ctctttccat
    30721 ctcagatgca tttctccagt caaagctatt taaaaaattc ctgattactg taagtgtatc
    30781 ttgagcttgt ttgtaagaaa tccaatttcc atcagctatt acttctatag ggtcaagttt
    30841 tccaaagcct attctctgat atgagccacc tgacaatgct gccgcataaa atcctagccc
    30901 ttccataccc ttatttatat aataatattt accatctgct cgaagctcaa ctccattggg
    30961 atttcctcca tagagtaaat cccatccctc tttacctgtt gtattaagac caagttgtgt
    31021 taacattcct ttaagaggat atttagggtc ataattagca cctttactta ttgcatttgt
    31081 ttgcactctg ccatccattg tgcctatatt agtctgatta cgttcatttg cactctgttt
    31141 tttcaagcta gatgtttcat cttcttgata tggctctgcc attactgtca tactcatcat
    31201 caatataata atcatggtaa tcatagtagt taatatattc ttcttcatac tactccccct
    31261 tatttaattc tttttaaaac ataaaactat taactcataa atatatggtt attagtaaat
    31321 tgaaattaag tctggttcat aaattattgg tttaatagaa gtgtattgaa attgtacctt
    31381 tatattttca ttatataata aacttatttt tttaaaacat attgtttaaa tcagtacatt
    31441 tatatgtttt ttttgttcat ctgttttatc aaatatatta gtctttgaat tttgtttgta
    31501 ttcttcagca tttttatcta acttttctat tctcgactca agtttcatta tttcttcttg
    31561 tgttaaattt ataaaatttt taagcatatt ttcaagctct tcacgagttt tacctactaa
    31621 agataagtcc tcagatactt tctctttttt attagaatcc tctttataat cctttttttg
    31681 ttctaactct tctaattttt catcatttaa tatttcttct tcaatttttt cattagactc
    31741 tttagtgatt aaagattctt tttcatcatt tgtatttaga taagttgaat tttcatctaa
    31801 cattgtactt ttatttttct cttctataat gctattttta atacctattt ctatattaga
    31861 aaaattatta gttgtttctc ttatttctga tttacataag ttcttaattg cgtcatttat
    31921 ttttttagta taaacctctt gtctatattt taaatctcta attctatcat taagttcttg
    31981 cttttgtttc ataatattag ataaataagt gtcttttcct acagtttcat ttttcttaac
    32041 catactttca tttattcttg tattatttat attactttca tatctaaatg aatattgact
    32101 tctatattga ctagatattt tcatttattt cacctcctta aaataaattt atgtttataa
    32161 aaaccaactt aacttaatta tattttatca tcaaaagtat acatttacaa atattagtta
    32221 atagttttat aggttcgagc attattttca acaaaaaatt tttattataa attaaaacaa
    32281 taatttttat aaatatatta tttgtatttt ataattcata tatttttact ttataaatta
    32341 gcaatattgc agttttgctc ttaagaaaca tctctatcat acataagcat atactctatt
    32401 atgtattagt ataaatatgc cttttcttat cttacataag acctctttat aaaggagcct
    32461 atcgtaacaa atatgtatac attatcacat gtatatatca tcatataaaa aatcaaactt
    32521 tattaaaaaa attaataaat tgctttaaat aataccacaa accaaatttg ccatcaaaat
    32581 cacattaaag taacaaattg atggcaaatt taatatatta atagtatata gactcataga
    32641 atgaacaaaa aaactacact tatcttctaa tcttcttatt tatttttgag atatttttaa
    32701 atttcttttc tttttttgct aagtattcgc ttttattgtc tgcaaatact gcttcagttt
    32761 taagcctacg ataggattcc catcttttag gcgaaagttc ttgatgagca atagcttccc
    32821 agatagcaca gcctggttct gaggtgtgag tacaattacg aaaatgacat tttccgaaat
    32881 aagcttctat atctgcaaaa cttttatcta aaccttctga aacatcccac attcccaact
    32941 ctctcatacc tggtgtgtca ataacaatac caccacctga aattaaaaat agctctcgtc
    33001 tagtcgtggc atgtcttcct ttatcatcat ctatgcgaat ttcctttgta tttaaaaatt
    33061 ctccacccat cagacaatta attaatgaag attttccaac tcctgaagaa ccaagtaagg
    33121 caacagtaca gcctggttta atatacttag aaatctgttt ataaccatct ggctctatag
    33181 atgaggtaac aactacatct acacccaagg ctatagaatt aactacttgt aatttacgat
    33241 gcacattgtc acataagtct gatttagtaa gtactactac tgggatagct ccactttccc
    33301 atgttaaaga cagatacctc tccaatctat gaatgttaaa atccttattc agtgacatgc
    33361 acaaaaaaac aatgtcaata ttagctgcaa ccacctgctc ctcatttacc tttccagcag
    33421 ccttacgcac aaacaagctt tttctatgaa gcacatgatg aataatagca ttaccatttt
    33481 tattattgca acgatccaac ataacgaaat caccaactgt gggaaaatca gattgtatag
    33541 tagcctcaaa acgcagcttt cctgaaacct ctgcaaacag tacaccattt acacatacag
    33601 cttgataaaa gttcttttcc tgcgagataa tccgagcaag gtataaatca tgatattcca
    33661 tactttgagt aataaaattt ctatttagac catagtcttg taaattgatt tgtttcatac
    33721 cattcattct gtttcctcct tttccaactt ttcaatgatt ctttctagtg ggaaaggggg
    33781 ctgtgccagt ggttttaatg caattgacat taatttcact gaattatcat cagctgcaat
    33841 acgattagaa ctaagatgtc cacagcgtgt acaacgatga ataattgccc actcaccatt
    33901 ttttctcacc cacactccaa taggttccat attagcatca caatctgcag cacggtcacc
    33961 aggaatgata tctacatgaa gactatgtaa acaatgtgga caatgatttc tatgttgtgt
    34021 accagctcct tcaggaacta caaggcaacc acaatttttg caaataaagt tatccataca
    34081 ggaattttcc tgataataag ctcgttttct atttttataa ttcattttta tttcctccgt
    34141 atcgtgtatt ttctacaaaa aaagtccaca ggtatttata tctgcctttt tatgtggtat
    34201 acatatttac ctacacaaaa acagaaaacc cctgtggact agaaaattta gtatcttcta
    34261 tttcaacata attatattca aaataatact gtgtaaaatt aataaaagtg tagcagatta
    34321 aagcccacta caccacaatt taacaaagta atatgtaata ttattatttg ttgtctaatg
    34381 ttagttatct ataaaggcag agtaagacac aacaatcaaa tgtatcaaaa atacacttaa
    34441 aaattctgat taaacagaac ccatatgcgt ctccaactat ttatttgttg gttaccatta
    34501 tatataactc tgacatcagg caagacactc cttcactaat aattattatt gaaattttat
    34561 cataaaatat aagtatgttc aagtataatt taatatattc atatatttat gtttgtctca
    34621 ttttcttatt ctataaaatc tagtggtatt ttgaaattta atgttcacct agtattaata
    34681 ataaaaatta actgctatta tttgttttct cattttatgc ctgttgctca atttatctca
    34741 cttaaattaa taacaactgt cttttcatac aaaacctaca atcaagtttt tccatatatt
    34801 ctacatgcat agtctatttt ttttgatata aaataacgtt catatttgta aatttattta
    34861 attttcaaat aagtatattg aataataagc aattctattg tattattgta attgtagaat
    34921 aaaactaaat cggcaaaact agagaaattt agtgacgcaa agctataggg actaacgctt
    34981 ataaaataaa ctatgagtta tgtcagccag ttgccaaaaa gagatattct ttttgttttt
    35041 atgaaagttt tttaggggga taataatata tatgtttaga gataaaatgg atagatgtac
    35101 acatatgttg actgcttata ttggtagttc atatgattat tgtgatttta tagatacaca
    35161 actagatgat tttatactag agtacggaga aaatgtggta gaatcttgtt tgcatcaagt
    35221 gatggtatta gtaagtaagt ataattaaat agatttggtt ttctagagaa ccctttttag
    35281 ttttatctac tttaaatagc cttatgttag aaagctaatt aagatagatt tctagtattc
    35341 ataattttat tttttattta aaatacatat aataaagatg taaatttgta tcaaataata
    35401 aatatgagaa cttaaattct cttatctatg tgaaaataaa catctcttaa aatgaaattc
    35461 tgtaaatata aatgattttt tagtttgaag gattagatta acaaaattat aaatttgcaa
    35521 ataagctata ttaaaaaata taataatttt ttatataact tattctttta taacttttaa
    35581 ttttaaaaga ggagaatgta tataagagta ttcaatgtac tagaaaaaat caaaagaaat
    35641 attagaagaa atggatattg agtaatcaaa gaaaagattt tgatattaca aatgatatgt
    35701 ttcaagaaag cattcctatc acaacaaaaa ttattcttga agacatgcct tcaaatgatg
    35761 aattaaatca tgttttttca aaaggttgtg aacgaaaaat gaaaaagaga aaaattgtat
    35821 taatcacact actattaata ggtgttttgt tattaggaag tatcctatat aatctttttt
    35881 taggaaaaac agctaatatt tcaatgctaa aggaatcttg gaattttgat ataccaatac
    35941 ctaacaaaga aattgaagta tttgatactc aagatagtat taatggagat ggacaaagtt
    36001 attttattca aggattttct gaaaaaaatt tcaaaaaagt ttttaattta aaaggtggta
    36061 tagttgtaag caaagataat attaatgaaa tagaaaaata tattgataaa tttaaaagag
    36121 attctgttaa cattaataaa agtaataaaa ataaaattga agaagatttt aaaaaatata
    36181 agttagaagt taaaaaagat gataagtata tatataagag aaattatgag aactacgtag
    36241 ttttaattat aaaaaaagat gaacaaaaat tatacagttt aatatggaat cagtaggtac
    36301 atatgtatta ttaagaatta taaataataa aaataaattt tagtcaatat acaaaaacct
    36361 tttaaattgt ctggaaatca cctcttcatt tgttataaaa acatacaaca aatgaagagg
    36421 tgattttatg gttttaaaaa aaataattat aagtggatat gtgctttcac atgtttatca
    36481 acatttgtaa tgcctatatt tgctaatgaa ttataaaaat attaaataat attcaactat
    36541 agattatgta aattactcta tgaaaaaaat gagttgtata aaatctggct actgtagatt
    36601 gactatttct tttaaaatta caattataat aagttttata gaattaaaaa taaaacttta
    36661 gagatttttg ataacattgt agactaaatt tgccaccgaa atcgctttac agtggcaaat
    36721 cggtggcaaa tacaatatat tattagtata taatctatat aaacattact attgtttgat
    36781 tatcttagct acagtttcca catgtgtaga atgagggaac atatctacag gttgtgcctc
    36841 aacacattta aatccgtgaa catttagata atctaaatcc cttgctaaag tagaaggatt
    36901 acaagaaaca tagacaattc tttccggatt cattgaaaca atagtctcta ataccttttc
    36961 gtcacatccc tttctaggtg ggtctacaac gactacattg gcagtcttac cctctctata
    37021 catcttaggt acaatttcct ctgcctttcc aacataaaat tcagcattat gtatattgtt
    37081 taatttagca tttatcttag catcttttat ggcatcatct ataatttcta ttccataaac
    37141 ctttttagcc ttttgtgcca aaaataaaga tattgtacct atcccacagt aaatatcaaa
    37201 aacagtatca gtatcattta aatttgcata ttcaagagcc ttatcataca atactttagt
    37261 ttggacaggg tttacttgga aaaatgataa tggtgaaatc tcaaacacta agtctcctat
    37321 ataatcgttt atttttccat ctccatatat aactatattt tctcttccta gaatagcatt
    37381 cgtatctttt tgatttacat ttacaaccaa agtcttaaat ccaggtatat tttccttaag
    37441 cactgaagct aattctttaa gatatggaag tttgtttcca tttgctacta atacaaccat
    37501 tacttcttta gttgtaaaac ctatttttgt tactaaatgc ctaatcaacc ccgtgtgagt
    37561 cttttcatca tatatgctta ccttataggc ccttatatat gtttttatta ttttaacaat
    37621 tttatcattt atctcatgtt gtattacgca tttttctgtc gatattacat catgagtctt
    37681 ctttttatag aagccaataa ttggaatacc attattcttt tgtattggaa attgtgcttt
    37741 atttctatat ctatatggat tatccattcc tatagtgtca tgaacttcta catcctctaa
    37801 cttacctatt ctcgaaagta cttgtttaac ttcatttgtt ttcatttcaa gttgtttatt
    37861 ataatctaag tcttgtatct gacaccctcc acaatcccta agattgtcac tacacactct
    37921 tttcaccctg aatggagact tttctattat ctcaacaata tctcccacag cataattttt
    37981 ctttacttta ttaatcctaa ctttaacttt atcatgtatt aatccgccct ctacaaaaac
    38041 tgtaatacca ttgtactttc ctactcctac tccgccttga ccaatgtcta ctatatctac
    38101 aatatacatc tcgtttttcg aaagcataaa tcaccctcca atcaatctat tatatttaaa
    38161 tctttacaaa cttttttctt taaatttata ttaaaatatt gctattaatt aaactatatt
    38221 ttttactaag ctacaatatt ttattttacc aaaaaaaaag gagcttataa agccccttag
    38281 taaataaatt tattattttt cttataaaac tctagttgaa cctctatata ccacgcctct
    38341 tgatgcatcc acagttacaa cttctccatc ttttactaga tttgttatat cagctgcaga
    38401 cacaataact ggtatatcta aatttatacc tacaatagct gtatgtgatg tcattccacc
    38461 attctcagtt actacagctc ctgctttttc tatataattg ttcatttcag catcagtagc
    38521 agtagaaaca agtatgtcgc cttcattaaa gtctacacaa gcatttccat tctttattat
    38581 acgaacttta ccttctactg tttgagtacc tacacctata ccttgcacga tttcttcgct
    38641 tattacatga acctttatta aattagtagt tccacttact cctactggta ctccagcagt
    38701 tataactact aattccccat tatctatata atcagcttgt ctagctgcct caattgcctt
    38761 ttctataact tcatctgtat ttcctgcaac agaagactta actggatata ctccccatgt
    38821 aagagctaat ttattcatag tcttttcatt atttgtagta gctattattg gagattttgg
    38881 tctgaattta gataccattc tagcagtata tcctgaagaa gtagatgtta ttatagctga
    38941 tgcatttaaa tctactgctg ttgtacaagt tgcatggctt attgcatctg ttacagtaac
    39001 attgttagta ccattttcct ttagtaatct atcataatct aaagtctctt ctgttctttt
    39061 tgctattgta gccatcattt ttactgcctc tacaggatat tttccagcag cagtttctcc
    39121 agataacatt atcgcatctg ttccatcata tatagcattg gcaacgtctg taacttctgc
    39181 tcttgttggt cttggatttc ttatcataga atcaagcatt tgagtggcag taacaactgg
    39241 ctttgctaat tcattacatt tttttatcat cattttttga actattggca tctcttcagt
    39301 tggtatctca acacctaagt cacctctagc caccattata ccatcagaaa cttttaatat
    39361 ttcatctagg ttctctacac cttcttggtt ttctatttta gatattattt gtatatcagt
    39421 tgcattattg ttctctaata cttctcttat tgctagtaca tcagatgcct ttcttacaaa
    39481 agaagcagct atataatcta ttccttgact tattccaaac tctatatcac ttatatcttt
    39541 aggagttatt gctggtaaat ttatcttaac accaggtaca tttactcctt tatgattttt
    39601 aactattcct gaattttcaa caacacaaac tatatcttct ccatttattt cttttactct
    39661 taagccaact aaaccatcat ctattagtat agaatctcct gatttaacgt cttcagcaag
    39721 acctttatag cttactgtac acatttcttt agtccccata atatctttca tagttatagt
    39781 gaacttttgc ccttcttcta aaaatacctc tgggtcttca aagttacctg ttctaatttc
    39841 tggaccttta gtatctaata atatagcaac tggcttattt aatttttctc taactttctt
    39901 tgccatatca attctttctt tatgttcttc atgcgaaccg tgagaaaagt taaatctaca
    39961 cacatttaga ccattttgca taagttgagt taaaacttcc tcactttgtg atgctggacc
    40021 tagtgtacaa acaatttttg tcttctttac attatttaac atatttgctc ctcctgagat
    40081 attattccac agatttattt ttaatactaa aacttatata gataatattt ttgccatctc
    40141 ataagcttct ttatcaaata ctttcttttg agctaaagct tcacttattt ctaaatcaat
    40201 tattttgttt tcttttattc ctacaactct agctgatttt ccatctaata ataattctac
    40261 tgctcttact ccaagtctac ttgctagtat tctatcagat acagttgggc ttcctcctct
    40321 ttggacatgc cccaatacag tgactcttaa atctgctcct gactcttttt taagttcctt
    40381 ttctaaatca ctagcacttc cgactccttc tgctaaaacg attatactat gtctcttacc
    40441 tcttttttga gtagtcttta atcttagagc aacttcatct actgtaattt ctacttctgg
    40501 tactattata gtttccgctc ctcctgcaag acctgcatat aaggctaaat ctccacagtg
    40561 tcttcccata acttctacta tgttaactct ttcatgagat gatgatgtat ctcttatctt
    40621 acctatagca tctataatag tattcattgc agtatcaaat cctatagtat aatctgtata
    40681 tgctaaatcg ttatctatag ttcctggtat gcctatagct gggaatccta agtcactaag
    40741 tttttgggct cctgcaaatg aaccatctcc accaattaca actaagcaat caattttata
    40801 ctttttaaga atttttactg cttttaatct accttcttct gttttaaatt cttcacatct
    40861 ggaagatttt aatatagttc ctcccctgtg tataatatct ccaacagaag ataagttcat
    40921 ttcagttaaa tcttcttcta gtaatccctt atatcctcta ttgattccat atactttgca
    40981 tccataatat attgctgatc ttacaaccgc tctaatcgca gcattcattc ctggagcatc
    41041 tcctccactt gttagtaatc ctatagtttt cattttttac ctccttggat tttatgtcaa
    41101 ttttttatta taatcattgt tattgtattc taatggactt tttttgtccc tatatcactt
    41161 tttaatccca tcaagactaa aaaaattaaa tgtccttaaa tgtccatttt gtgatattat
    41221 atcacaaaac ggacaatatt tttcatatta ttataatata tttagatttt tatataaaaa
    41281 atcaagcttt tacttttatg ttatctttag gaagtaactc ttctaagtta tttataaatt
    41341 catcacatat acaagtcaat gccccggtca ttttatatgc tttacgttta ccatttatat
    41401 tttcatccat agggtataag tatatactgt cattacctgg atattgtttg gctatttcta
    41461 caatattatt agttgtatca gtatcagaca tactatctat tttcaaatac aattttttac
    41521 cactttctgg cttaatattt tctacatttc tagttcttcc tgaattgtag tttctatctg
    41581 caaagtcagt atcatcattt atatctttta tctctctagc tataagtttt gcattttcgc
    41641 cttcttttat acttaatgtt ccttttacat atattatatt atcttcattc aaaatgacat
    41701 tatacttttg taataattga ggaaaaacta ttatttctat agcaccatac aaatcttcta
    41761 attcaataaa tgccataatt tcatttcgtt ttgttgtttt aatagtttta gttacaatca
    41821 ttccacccat tataacatct gtttcattca tttgtaggta tgactcttct tcctctttta
    41881 atgaatttaa ttttccatta tccatagatg ttttttcttg tagttctttt tcaaattgtg
    41941 ataaaggatg tccacttaca tacattccca agacttcttt ttcaaggtta agtctctctc
    42001 tttcttcgta ctcttgcctt ttaggcaaac tgtatatttg ttgaaaatca ctatcttcct
    42061 cactatctcc aaaagcatca aataaagaaa tttgaccttg aacatttttc tttctatcca
    42121 tagatacact ttctaataaa ctttcataac cagccatcag actagctcta ttttcactta
    42181 tattatcaaa agctccacat tttattagac tttctattac tctcttattt gtatcttttt
    42241 ggtctaatct tttgactaaa tctactaaat ctacaaataa tccattagat tctctttcag
    42301 cgattatatt gtttattata tttacgccaa cattttttac ggctgctaga ccaaatctta
    42361 tattgtcgcc ttcaactgaa aactttgaaa aacttttgtt tatatctgga ggtaatacat
    42421 ctatttctag tgctttacat tctcttatat actcaactac cttatcagta tttcccatta
    42481 cactggttat caatgctgcc ataaattcaa ctggataata tgtttttaga taagctgtct
    42541 catatgctaa aaccccatat gcagctgcat gtgatttatt aaatgcatat ctagcgaagt
    42601 ctatcatatc atcaaatatt ttattagcta tttcctcttc tacaccattt ctgatacacc
    42661 ctgcaatttc tatattacca tcttcatcaa atttaccatg aataaaatat tgtctttctt
    42721 cttccatcac gtccatcttt ttcttactca tggctcttct aactaggtca cttcgtccgt
    42781 aactataacc acctaaatct ctaacgacct gcataacctg ctcttgatat accaaacaac
    42841 catatgtaac ttccattata ggctttaatt tttcatgtag atatgttaca tcttttgggt
    42901 tgcttttatt ttttatatat gcaggtatag aatccattgg acctggtcta aatagggata
    42961 ttcccgctac aatatcttca aaattgtctg gttttagctg tttcataaaa cttctcatgc
    43021 cagcactctc caattgaaac acccctagtg tatttcctga tgaaagcatt tcatatactt
    43081 tcgggtcgtc ataatccatc tttgaaaagt ctattttttc tgtgtaattt tttataactc
    43141 tatttttttc tattaaatca agagtatctc ttataactgt aagagttctt agcccaagaa
    43201 aatccatctt taaaagccca agttcttcaa gtgttgtcat agtaaactgt gttgtgattg
    43261 catcttgatg tttgtacaaa ggtacatatt catctacggg attttttgat ataacaactc
    43321 ctgctgcatg agtagaagca tgtcttagca ttccctctat ctgttttgat atatttatta
    43381 tttcttttgt ttccgggtct tggtcataca attctttaag attaggattt gtatctaggg
    43441 ccttatctat tgtcattcct aaagcaaatg gtatttcctt ggctaccttg tcaactttat
    43501 tgtagcctat atccaatacc cttccaacat ctcttattgc agcttttgca cccattgttc
    43561 caaaagttat tatctgggct acatggtcat caccatattt tctctttaca taatctataa
    43621 cttcttctct tctctcataa caaaaatcta tatctatatc tggcatggat atacgttctg
    43681 gatttaaaaa acgctcaaat aatagagagt actttattgg gtctatatca gttattttaa
    43741 gtgtatatgc tactatcgaa ccagccgcac tacctctacc tggtcctact ataatattat
    43801 tttctttaga aaaatttata aaatcccaaa caattaaaaa atactctaca taacccattt
    43861 tttcaattac atctaattca taatttaatc tctctagtat ctcattacta ggattatcat
    43921 atctctcttt taatccatta aaacacaact cccttaaata agtttctggt gtatatcctt
    43981 ctggaacatc atacttagga agatgtatag tattaaaatc aaactccaca ttacacatat
    44041 cagctatttt cacagtattg tcaatagcct ctaatgcata tggaaataat tcttccattt
    44101 cttctcttga ttttaaataa aactcatcac taccaaatct cattctattt gggtcattta
    44161 atgtttttcc catttgaata cacataagta catcatgtat tttcgaatct tctttattta
    44221 catagtgaac atcattagta gcaactagtg gtataccagt ctcttttgat aattttacta
    44281 aaccagagtt tacttctttt tgctcaggta aattgtgatc ttgaatctcc aaaaagaaat
    44341 tttcttctcc aaatatgtct ctgtagtcaa gagcaaattg ttttgctttt tcataatttc
    44401 tattcattaa agcacgtgct acatctcctg caaggcatgc agaaagtgct ataatccctt
    44461 tgttatgttt tttaagttct tccatgtcaa ctctaggctt atagtaaaaa ccttctacgt
    44521 atgaagttga aactattttt attaaatttt tatatccttc catattctta gctaacagaa
    44581 ctaaatgtcc ttgatatttg tcatagtttg ggtctttatc tctcaatcca cgtgcagctg
    44641 tatagacttc acaccctata attggcttta ttccttcttt tatagctgtt ttgtagaaat
    44701 ctataacacc aaacatacaa ccatggtcag ttatagctat tgagctcatg tttagttcct
    44761 tagccctttt tataagtttt ttcaccctcg aaaacccatc aagtaaacta tactcagtat
    44821 gtacatgaag atgagcaaaa tctttgttca ttaaatcacc tcctgcttta taattttaat
    44881 tttaccacaa atatatgtcc taaaacaaaa acagagactt ttatctcaaa ataggaatta
    44941 acttcaacgt ataccattat atgttataaa ttaatgctta ttctgagatt gtcccctcaa
    45001 tttattgcta tgattttttt ttcaaatctg ttgcaatttc ttctattttt ctaagtctat
    45061 gattgactcc agattttcct attggtggtt ctaacatctc tccaagttct ttgagagtca
    45121 tatcctcata ctcgattcta atcttagcta tctcttgtaa attttctggc aaactgctaa
    45181 ttcctatagt ctcttgtatg agcttaatat tttctacttg tcttactgct gcatttactg
    45241 ttttagaaag gttagctgtc tcacaattta ctatcctgtt tacattattt ctcatttctt
    45301 ttactatttt agtattttga agacttaaaa gagcgtggtg tcctcctata atactcaaca
    45361 aatctgatat ttgttcactc tctttgatat aaacaacgtg attatttttt ctagccacaa
    45421 ttttactatt aaaacctaaa gagtttataa gttcttttag agaatctgca aactcctcat
    45481 tattagttac aaattccata tgatagttct tttctgggtc gcttatagaa cctccaccaa
    45541 gaaaagctcc tcttatgaaa gctctcttac atccttcatg cttaattaag ttctctgaaa
    45601 ttttgtttat agtaaaaaat ccttctttat cttcaagtat acctaacttt tttaataaca
    45661 tttctgaacc catttcgctt gttaccatta atacataact gttatttctc tttagcattt
    45721 gattcttatt tactgagatt gtagtattga tattaaaatt cttttttaat aatttaaata
    45781 ctttacgggc tattgaattt aactctgttg ttatctttag atttagcttc ttatatccaa
    45841 caatttgtat acttccccct gatttaacta gagctgacaa ttcagctata ttacaacatt
    45901 cattcccaga tataactctt gctagttcat ttttggtttc agttgaaaaa gacatatcta
    45961 tcacctatta actaattttt ctttaacctt ttttttattt cttgtaaggc aacctttatt
    46021 atctcctttt catcatcata cctagctaag tctactactc tatctaggtg gataaattta
    46081 gcatcaataa agccttcctc tttttgtgga atagtatcca tattctttgc ttgcatcaaa
    46141 taccaaaaaa ctgttttatg aactgctcta ttttcatccc agttttcttt gaatgtatag
    46201 tgtatttcac ctaaatattt caatatatct gcttttactc cagtttcttc acttacttca
    46261 cgtaatgctg cctcttggtt attttcccct tcttctactt ttcccttggg taacacccaa
    46321 tctccattga acttccttag taaaagaatc gtattgccaa acaggactac accaccagca
    46381 ctaacctctt ctctcatcga tgttcactcc ttaaaactaa cataatgata aggatttcta
    46441 cacaattttt taaaatcctt ttttactaac ttttgttgta attatgattt agggctaact
    46501 caataactat gttagatata tactttgcat catgtcttat ataattattt tttatttcaa
    46561 ttaaattctt ctctacagtt tttataccca tctcttttaa tttatctttt tgctttttat
    46621 ctaacaagac ttgctctgct ccatcttgtt tatataagtc aagcatttct tctggcaata
    46681 tctcattatt tgcgattaca taatctatta aattctcatc agtgtgtttt actatagcat
    46741 taacatgctc taatacattg tacccttctg tctcacctgg ttgagtcatt atatttgcta
    46801 tatatacttt tggagctaca gacgatttaa tagcgtctac aatcccctct acaagtaaat
    46861 ttggtattat acttgtatat aaacttccag gacccataat tataatgtca gcatcataaa
    46921 tagatgcaat aacatccttt aatggtttta catcttttgg atttaaaaaa attttatcaa
    46981 tagagctttt ttgaattttt gactcttcag gtatactaga ttctccattt attatattgc
    47041 cattttctaa tttggcaact aaatttacat cttccaaagt tactggtaaa actcttcctg
    47101 ttatagcaaa aatttcactt aatttatata cagctttttc aaagtttcca tacaacccat
    47161 tcatagcagc caaaaacaaa tttccaaaac tctgaccttt taaaagccct tctgtaaacc
    47221 tatattgcat aacttcattc attgttggct ctatatttgc aagtgctaat aaacagtttc
    47281 ttatgtctcc tggtgggagc attcctaagt cttctctcaa tacacctgaa cctccaccat
    47341 catcagcaac agttacaatt gcagttatat tttgcgtagt gtgtttaaga cctcttaaaa
    47401 ataccgattg accagtacct ccacctatca caaccacatt tggttctcta ttttcaacaa
    47461 tagacctttg aaatagtttt actttttcat tttttgcttt tttatatata aataacgata
    47521 caattaatgc aatccaacct aaaattccta caataataag taatttagcc atgtttgcct
    47581 ccattatcgc aacatagagt ctctatgttt ctttacaact ctatatccct tattagataa
    47641 atcctccgct ataagatttg ttatagtaac tgacctatgt cttccacctg tgcatccaac
    47701 accaattact agatgttgtt ttccttcctc tatatattga ggtactaaaa aatgaatcat
    47761 atctaaaagt ttcacataaa attcttcact gattttagaa ttcataacat aatctctaac
    47821 ttctttatca tcaccagttt tagctcttaa ctcttcaaca taatagggat ttggaagaaa
    47881 tcttacatca aatactaaat ctgcatcagc aagtatacca tgtttaaatc caaaagatac
    47941 tacagaaata gttaggtttg gattatcttc accagaagaa tatattttct ttatttcttc
    48001 cttaaggtct ttaggtttca tgttagttgt atcaattata catgtagata actcctttaa
    48061 aggttccatt atctttcttt ccatttttat accttctgga atctgcatat cttttgccaa
    48121 tggatgattt cttcttgtca ttttatatct ctttagcaat acatcatcat tacaatccaa
    48181 atataccatt tcatattcat aattttcttt ttctaaataa tttaagcttt catgaagcgc
    48241 ttcaaaaaac tttctccctc ttatatctat ccctagagca actttatcaa tacttgaatt
    48301 tggttgataa cataattctg caaatttagt tatcaaagca ggaggtagat tatctacaca
    48361 ataaaatccc atatcctcta aagctctcat tgtctcactt tttccagatc ctgaaagacc
    48421 tgtaactata acaaatttca ttgctaactc ctccctttta tcttatgttt attatattat
    48481 aatgttttct actaacgata tatccagatt agagtcagat atagtcttca tagctagttc
    48541 aaaatcacca actctttctg gacaatgtgc atctgagcct attataaatt tattttctag
    48601 cccttttatt ttatttaatt gatttacatt caaaaaagaa tggctactat ttatctcaag
    48661 tctagtattt gtcttttttg ctgtctttgc tacttcttct atatacacat cgcctttatc
    48721 tcctggatga gtaattataa aaatatcatt attacttatc gcatttatta cagcttttgt
    48781 attatattct tttgcttttt cagttttaaa aatatagtta ttacaatgat ttaataagct
    48841 tcttaatgat gttggagtag aaccaaaatg atatccagcc ataatataat caaaatctct
    48901 aataaattta tcatccatat ctatatttcc tttatcatct aatatgttac actccatacc
    48961 taaaagtata tttatatcag tgtacttatc atttaaataa tctacttctt ctctcatttt
    49021 gagaatatct tttttcttta caccatatgc aatatgttta taactatggt cagatattcc
    49081 tattgttttt attcctttag aaatggctac ctttacatta tcctcaatag tccctttgcc
    49141 atgactatat aatgtatgtg tatgataatc agccagtatt ttcatttgtt aatcctctcc
    49201 aagtattttg acttcttctt ccaacataac cccaaacttg gcatatacag cactttttat
    49261 aacatatatt aaatccaaaa tatctttagc acttgcttct ccttgattga ctacaaatcc
    49321 acaatgcttc tcagatactt gtgctcctct taaagtaaga cctctcaatc cagtttcttc
    49381 tattaattta cctgcaaaat atcctgttgg tctcttaaat gtacttcctg cactagcaaa
    49441 atttaatggc tgtttagtta ttcttctcgt cgctaaatca tccattaaac tttttatttc
    49501 atcataattt ccttcttgta attctaattc tgctgataaa gctatatatc cgtttttcga
    49561 tattatactt tttctatacc caaattccat ctgttcattt gataattcaa atatattgcc
    49621 ttccatatcc atcagtctta ctgacttcac tatatccttc atttcaccac catatgctcc
    49681 agcattcatg gctaatgctc ctcccaatgt gccaggtatt cctgatgcaa actcaaaccc
    49741 ttttaattct tgcttttgga gagctttccc taaaactgat aataaagctc ctgactgagc
    49801 agtcatccta gtatcattta tctcataaga attgaaatta tcagctaatt ctattaccac
    49861 tcctcttatt ccaccatctt taattaaaat gtttgaacca tttccaatta ttaaatacgg
    49921 aatagactct ttttttacca acttaagtac attttttaat tgttcttcag ttctaggtct
    49981 aactaatata tctgctggtc ctcctaccct aaatgaaata tgttttttca taggttcatc
    50041 gacttttata tcctctttac ttaatatatt taataaattt atatatatat cttgattatt
    50101 cattgtattt cctcccaaat tatactgaac tttagctgtc attattattt aaattacatt
    50161 aatatatagt attaactata ttcaataata tatgattttg cttatcatat ggattttata
    50221 ttttaagctt cttaattaca aactatattc aataaatata taataaagta aaaaaaaaaa
    50281 aataacaaca tataaaagaa tataaataat ttaaaaaaag catcaatatc actgatatca
    50341 atgctttaaa aggcaattta attcgtatta tttatctaac tatttttgac atagcaaaga
    50401 taataaatcc ctccaactac tatggaacct ccaacgatat ttccaagtgt tacaaatacc
    50461 aaattagtta taaattgaac catagttata tttgctccta atgccattcc cattggtata
    50521 aaaaacatgt ttgcaacact atgttggaat ccacataata caaatagcat tattactgtc
    50581 caacatgaag ccaattttga cattatatct tgagatgctg tagccatcca tacagctaaa
    50641 acgactagaa tattacaaag tattgctttt aaaaacattg atgctatagt tgaattaacc
    50701 ttagcttctg ccacagctat agcttttgta gctgcatctc ctgtcaatgt agaagaataa
    50761 aacacaagtg caactagcaa aattgaacct actaaatttg caaaataaac cagtccccag
    50821 tttcttaaca tttttcctaa tgttattttt ttgtccataa gtgcaatagc cattaaagtg
    50881 tttccagtga ataattctgc tcctgccact actacaagca taagacctac tggaaagacc
    50941 atagcactta aaaagctagc tacccctggg tctatcttgc caaaagtttg actaactatc
    51001 atactcccca atgccccaaa acctataaat gctcctccaa ataaacctaa tagaattgca
    51061 ttttgtgtcg aaatatttgc ttttttaatg cctgcactta tagttgcttg tgcagtttcc
    51121 ccaggtgtca agaatccttt actcataaat ccttccccct taaaaattat actttccctt
    51181 gtctttttat ggcaataatt atataataac aatatgatat ataataaatt ttattaacat
    51241 aaaaaat
//
LOCUS       DAMJVK010000015        50560 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.015, whole genome shotgun sequence.
ACCESSION   DAMJVK010000015 DAMJVK010000000
VERSION     DAMJVK010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 50560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50560
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.015"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(111..2081)
                     /gene="ftsH"
                     /locus_tag="PC414_000899"
     CDS             complement(111..2081)
                     /gene="ftsH"
                     /locus_tag="PC414_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDF2463308.1"
                     /translation="MNKLLKGAGFYLLVFIIIVGIVQFSGKPTEKIKDLKFSEVYREL
                     TDENISRLYFVNQTSVEGTIKDTNTKFKSYVPTEVMGNKLADEVLDQAKAGKLTFGGE
                     AKPSTPWFVEMLPTLLLIFFMVIIWFVFMNQSQGGGGKVMSFGKSKAKVHKDDEKTRV
                     TFKDVAGLDEEKEDLQEVVDFLKNPKKYIELGARIPKGMLMVGPPGTGKTYLSRAVAG
                     EAGVPFFSISGSDFVEMFVGVGASRVRDLFEQAKKSAPAIIFIDEIDAVGRKRGAGLG
                     GGHDEREQTLNQLLVEMDGFGVNQGIIIMAATNRPDILDPALLRPGRFDRQVVVGTPD
                     VKGREAIFKVHSRNKPLSDDVKMDVLARRTPGFTPADIENLMNEAAILTARKREKKIK
                     METIEEAITKVIAGVAKKSKVISEKERRLTAYHEGGHAVCAHVLEEVSPVHQVTIVPR
                     GRAGGFTMQLPVEDKFYATKNEMKENIIVLLGGRVAEELVLKDVSTGASNDLERVTAT
                     ARSMVTKYGMSSKLGPMSFDSDDEVFLGNSFSSKRNYSEEVAFEIDQETKRIVDGAYD
                     KTRSILQENMDRLEYVAQALLIYETLDAEQFVKAFNKELPLNEIENAVTEENSSKEVE
                     EQLTIKLEKDEEERNNVIDINKNLEDKSDKDK"
     gene            complement(2115..3503)
                     /gene="tilS"
                     /locus_tag="PC414_000900"
     CDS             complement(2115..3503)
                     /gene="tilS"
                     /locus_tag="PC414_000900"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HDF2463309.1"
                     /translation="MIFDKVLSTINKHNLIQKGDKIVLGLSGGPDSVCLLHVLNRLKK
                     DFNIEIYAAHLNHQIRGIEAQKDALYVSKLCEDMGIIFFVKSINVPKYCENEGLSLEE
                     GARKLRYEMFYEIKDKIKANKIAIGHNLNDQAETVMMRIMRGTGLKGLKGIDYIRDNC
                     IIRPILDVERNEIEEYCEAYNLNPRIDKTNLENIYTRNKIRLDLLPYMKDNFNSNVIE
                     SIVRMSNSLKSDNDYIEKEAEAKFREVSNIKEKGFVEINLDDFVCLHDAIKVRVLRNS
                     IKHILGDTNFVDQRHIEDIMSLEDNSKVNKMLTLPRNIFVYRKKDSIILTNEEIVNEE
                     IEFYYNVPSNGFIKIKELKQIIETQVMSIDRYKSMKLDNSSKGFDFNKVKGGIVIRSR
                     RQGDKIKLAMGSKKVKDLFIDLKIPREERCKIPIITDGEGIICVGDYKISENYKIDES
                     TKEVLKINFNKL"
     gene            complement(3686..4114)
                     /locus_tag="PC414_000901"
     CDS             complement(3686..4114)
                     /locus_tag="PC414_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HDF2463310.1"
                     /translation="MEEKSLSVKISINTRQYDEKGNMDTIEMTAFGKIFYKNDGIYVI
                     YKEKEEDIEITNTIKILKNQVSIKKFGAINSTMLFKCGESSTTKYVTPQGTLLIDIDT
                     RELDINIQEGEHIKLKIDYNIKVQDLFLGRNKIDIYIGTK"
     gene            complement(4131..4937)
                     /locus_tag="PC414_000902"
     CDS             complement(4131..4937)
                     /locus_tag="PC414_000902"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDF2463311.1"
                     /translation="MNNKPIGVFDSGLGGLTVLKEIMKILPNEDIIYFGDTARIPYGS
                     RSKETIIKYTFQAINFLKTKGVKAIVIACNTATARSLKEAQEKYDIPIIGVIEAGART
                     AVSSTKNKIVGIIGTEGTISSKAYNLEISKIDESIEIVNKACPLFVPIVEEGWANTEV
                     AKLTAKIYLQELKEKNIDSLVLGCTHYPILKRTIGEEVGEHIKLVNPAKETAKDLKKI
                     LEVQNIINNTEIHGTYQYYVSDIPEKFSDIAREFLKKKIDKIQKVEIQKY"
     gene            complement(5139..5624)
                     /locus_tag="PC414_000903"
     CDS             complement(5139..5624)
                     /locus_tag="PC414_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HDF2463312.1"
                     /translation="MATLLALTFIFMPIKEVFAYEPTENIKKVNKQEKNIAKKEVINL
                     SDNDLMLLSKLVAGEARGESYEGQVAVAAVVINRVLDPRFPDTIEEVIYQKNAFSVVL
                     DGSIKMAPTDSARKAAQEALYGTDPTNKAVYFWNPEIATCKWINRLNPYMRIGNHVFA
                     K"
     gene            complement(5675..6334)
                     /gene="spoIIR"
                     /locus_tag="PC414_000904"
     CDS             complement(5675..6334)
                     /gene="spoIIR"
                     /locus_tag="PC414_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HDF2463313.1"
                     /translation="MRKIKIRLGLLILSLISVISIMTIVINGEVKKVDNISKDYKDKL
                     IRFHVIANSNTDEDQELKLKVRDEVIKYLQPKLQNSKSIKESEAIIKKEYSSLEEISK
                     NIILKNGYNYSVKVGIQYSNFPTKQYSNIVLPAGEYKALKIIIGKGEGKNWWCVMFPP
                     LCFVDESNGVIDKSTDDKLKEVLTDKEYKLIKQDTPKKTSRVKIKFKVLEVVKDLEKK
                     F"
     gene            complement(6479..7159)
                     /locus_tag="PC414_000905"
     CDS             complement(6479..7159)
                     /locus_tag="PC414_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2463314.1"
                     /translation="MENLTKLNLDNYKPLRDVVFENLREAILEGKLKPGQRLMEVQLA
                     EQLGVSRTPVREAIRKLELEGLVVMLPRKGAYVANMSLKDIMDVLEVRASLEGLAAYL
                     AAERISDEDIKKLKDISEEFKKSTLESDVDALLKLDVEFHECIFKATNNKKLIQLINS
                     LWEQVYRFRVTYMSAYDTSHNIVEEHKYLLDAIIKGDSELSKKYATEHIEKAEQFMID
                     KAMISKDL"
     gene            complement(7143..8033)
                     /locus_tag="PC414_000906"
     CDS             complement(7143..8033)
                     /locus_tag="PC414_000906"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HDF2463315.1"
                     /translation="MDFIRLKSRAKINLSIDVLGKRQDGYHFVEMIMQTIDLYDIVKI
                     KELDEDEIKVKSTSLDIPLDEDNIVYKAAKILKNKFYIKKGVEIFIEKNIPVAAGMAG
                     GSSNAAAVLVGLNHLWELRLSEDELKEIGLNLGADVPFCISGRPALAQGIGEKLTNIK
                     GLPCDTNILICKPDLFVSTKEVYQGLDLNNIKKRPNNKYLIECLKSEDIKAVSESMVN
                     ILENVTIGKHKEISDIKQVMMKNNALGSMMSGSGPTVFGLFKNKEDALIGKKELLKKY
                     KQVYVVNSSQKGVEICGEFN"
     gene            complement(8151..9701)
                     /locus_tag="PC414_000907"
     CDS             complement(8151..9701)
                     /locus_tag="PC414_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HDF2463316.1"
                     /translation="MELIKDVIKVDNRIDFGKFQTFIEAEAVVPDKKLDVYEIVKTEG
                     YISLKKIEMADGKILCRGSFNYNVIYIADDKNTISNVDGKVDINEVIEKDNVIQDMEY
                     MLFSEVEHMDCTIMNERKIKVGALMNIRGSLFEKQRLDIVKDVAQVECIQKHRKEICF
                     QDIVGIEKAESSIRDTITINTEEIQSIISLNPCVKVKESRVTDNKVIIGGVLEINPLA
                     CTYEGELVELDRVGIEFTQFVEVPGVSDGMTEEVLLSMSDFNHIFKQNSESNTGLLEI
                     DCMACCKVKVTDEVTREVLQDAYSPQKIIKFDHKPIQLNKTLRRSEETFMVREGIRND
                     NDDIQIKDIVSVCPTMSIENSYIEGNKSIIQGIIKVEILFVPVEGLKLVYKISEEIPF
                     EHDIEMDNLTDTASVFNTACIDKVEVDLNRDQIDLIIKIKRFTEALDKKAENFIIKGE
                     DQGVYDLSKAPSIIVYICKEGDTFWNIAKKYNTTENEIAELNDIKLDEPIKPGKCLIL
                     EKKVVLVD"
     gene            complement(9795..10061)
                     /locus_tag="PC414_000908"
     CDS             complement(9795..10061)
                     /locus_tag="PC414_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HDF2463317.1"
                     /translation="MATVQTLDKIRVSLERHIGKKILLKANKGRKQIITKEGILEKVY
                     PSVFVIKLDDESNGYPRVSYSYSDLLTSNVKLQVFRDQDKLQIS"
     gene            complement(10209..11069)
                     /gene="yabG"
                     /locus_tag="PC414_000909"
     CDS             complement(10209..11069)
                     /gene="yabG"
                     /locus_tag="PC414_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation peptidase YabG"
                     /protein_id="HDF2463318.1"
                     /translation="MKVGDIVARKSHNKDIVFKIVSFGVDENNEKIAILKGIAFRVIA
                     DAYIDDLELVKAPDIKDILIDKNVENLLYKSVRKAKERQKRMTRAVPKLQLNPNTYGM
                     PGKVLQIDGDKEYLKICLDVYAQLGIPAVGVAVSEQNQYKEVRALLEKYNPDILVITG
                     HDAMTIKRGDIEDMSNYRNSSNFVKTVKEARKWQPNLDNLVIFAGACQSNYEKIISAG
                     ANYASSPGRIMIHALDPVFIVEKIACSRIDVVVPIDEVIEQTITGVKGIGGSETRGKF
                     RWAMPKTILH"
     gene            complement(11153..12091)
                     /locus_tag="PC414_000910"
     CDS             complement(11153..12091)
                     /locus_tag="PC414_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HDF2463319.1"
                     /translation="MKFLDLHCDTIAKLMENVETSELKSNKYSVDIDRLKKGDSLAQT
                     FALFVDTEEVKHPFDYCMSMANKFHEEMKKNSDEIALATNYEEIMKNQSEGKLTALLS
                     IEEGAVLEGKLENLKKFYDLGVRMMTISWNHVNELSFPHNKVEYREKGLTDFGREVVH
                     KMNELGMLVDVSHISDGGFYEIAKISSKPIIATHSNSRAMMNHSRNLTDDMIKVLANK
                     GGVTGINFFHLFLSDKSESKLEDMVRHIKHIVNVGGIDVVSLGSDFDGIDSKVEIEDI
                     SQMGKLYEPLKKEGFSEDDIEKIYYKNALRVIKEVL"
     gene            complement(12201..12362)
                     /locus_tag="PC414_000911"
     CDS             complement(12201..12362)
                     /locus_tag="PC414_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463320.1"
                     /translation="MFILRFADDDDNLSVKDFTSLTDLKKYISENNIDKTWHQIEEVK
                     KVIPNLKEE"
     gene            complement(12372..12869)
                     /locus_tag="PC414_000912"
     CDS             complement(12372..12869)
                     /locus_tag="PC414_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HDF2463321.1"
                     /translation="MSLYTEWRNLSDNHESQEAEVKFWEEYLKVEAGIYNEILNNKMD
                     VVEGKVSELAEKFGTTNEFFMGFIDGISESLKEDIVLEEVEADKEVSIKIDWEKLYNN
                     MVTVEAHWLYNLQGWEDILPADQRKEIIKAYKKSKTIVKEAKIGRNDSCPCGSGKKYK
                     KCCGK"
     gene            complement(12984..13694)
                     /locus_tag="PC414_000913"
     CDS             complement(12984..13694)
                     /locus_tag="PC414_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0489 family protein"
                     /protein_id="HDF2463322.1"
                     /translation="MENYEYSGFYIEKPVGNNVFSYDKRENKSIYVPKLINGTLNDVR
                     LGNEVVFNEVDENKEIKAKGLENMVEYVLGNKKIYVFDNHNHAFYFWAKSLLKGEFTK
                     GCKLVHVDQHKDTREPENYDVDVNNLKDVFRYTNEVLNVGSFIKPALKYNIFSELIII
                     DSLYGFDLEVESEFVLDIDLDIFSSDMDYIPFELKFYKIKNLIKKAKVITIATSPYFI
                     NQEYAIKVLKELFNYDII"
     gene            complement(13870..14400)
                     /locus_tag="PC414_000914"
     CDS             complement(13870..14400)
                     /locus_tag="PC414_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463323.1"
                     /translation="MDIRALPIRITKEEAIEIAKSGKNSLFSKLILKNKYAHEVRLHY
                     IEFKIITLKIHKTKSIFKNRRYSNDINELKILVNGSTGSGSIVQDFPTYEVVKNVDCN
                     IVQYSDKDERRVKAKANKMAMRLTHRFMGGIPEIEIVKIESIFRPYWVVFFGKVELNR
                     RVSYLPIEADGFVIGM"
     gene            complement(14417..14647)
                     /locus_tag="PC414_000915"
     CDS             complement(14417..14647)
                     /locus_tag="PC414_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463324.1"
                     /translation="MSKLVSKVKKDLEIKWLGIDGFLVLLYFLITGFIFYGILTRNDI
                     IVGKIIPYGVMAVVIVIFFDYIYRVLKMTRKK"
     gene            complement(14662..16056)
                     /locus_tag="PC414_000916"
     CDS             complement(14662..16056)
                     /locus_tag="PC414_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HDF2463325.1"
                     /translation="MLTFWENGIIVIFAFILLGIGYYFSRNVKDMESYYLGNRSLPWS
                     LIVGTLVASWYGGVGTVGSVEYAAIYGISAWAVWSIAAHIGRMPLALWVGPRIHIRTN
                     ITVPDLLESFYGKHVAIIGAILMFIYCAQLGEITAMGTIGNVAWKIDKELIGLIMVGV
                     VVVLTVLGGLMGVAITDMVLFFCMVFGLTMVMPGQFEQIGGFQGLTEALKDAPQLMHP
                     TKGMSAMKALMLIVYSFGAYSDPTFYQRFSAADSPKVGRRALLTCFSLWICFDMVLTM
                     TGLIVRATYPEMAPAAGYITLVLDTLPQGIRVIFIIGILGSIISTLDSYYLAAGATLA
                     NDIYGRITKKELSQKQLVKFTRIGVCLAAVMGLSVAFRFENVYDACIFVGSIWMGSAF
                     VPIVGGLLGNGRRTSMGGILGMIVGGVTFGYFKIFPVQSFELEPLVIAIPFSFIAWMI
                     GNRIGENLNTQNNL"
     gene            complement(16182..17435)
                     /locus_tag="PC414_000917"
     CDS             complement(16182..17435)
                     /locus_tag="PC414_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2463326.1"
                     /translation="MESKNNVKRFLIIFILAFGTTAMYSLPYMKSSFYDPMQQALALS
                     HTQIGNLLSLYGLVGMVSYFIGGWFADRFSVRKLITFSLIASGILGFYFSTFPSYSMI
                     LLIFVLWGFTTILTFFSASVKVVRMQGSESEQGRIFGFYEGLSGVSGTLISFIGLYFF
                     GKFAEITVGFKYVVWLYSAASIICGILLFFLVEEKKDSGASDEGLSIKSLLKVVTIPK
                     AWLIGLIIFSTYLVFSSLTYLSPYLSEVYVMPMTLVSALSIIRTYVIKMGASPVAGVI
                     TDKVGSSIRVMFVGFILMTVSTAAYLVIPKSTGFIWIAVINMIILSVILFGFRGIYFA
                     SVSESNISLETTGAVVGFASFIGFSPDAFYYTIAGNWLDKYGQTGYTYIFILSVVCAV
                     IGIFATYALNKINKRENKGLNNKIA"
     gene            complement(17486..18640)
                     /locus_tag="PC414_000918"
     CDS             complement(17486..18640)
                     /locus_tag="PC414_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF2463327.1"
                     /translation="MSNNINQDLETKIIHWGHSADPTTGALATPICQTATFAAKTVEH
                     FEELCMTWGYVYTRECNPTLTELEAKLAMLENAESAISSTSGMGAITSTILALVKSGD
                     HIVSSDGIFSHTKLFMSELLSKFGVEVTFVDAVNPQNVKEAMRPNTKIVYIESPLNPS
                     LDLVDIKTIAEIAHENKSLAIVDSTFGTPIVQRPIDLGADLVIHSLTKFINGHGDTLG
                     GAVAGSKELIDLVRWPSLCCFTGASLPPMSAWMILRGMKTLDMRMKKHCENGLAVAEF
                     LEEEENVELVKYPALKSHPQYELCKTQMNGLGGGVVSFKLKDGISGLTRDQASRRLMN
                     SLELATIATSLGEEHTLVQMNGENLIRIAVGLESSNDIINDFKQAIEKLK"
     gene            complement(19200..19769)
                     /locus_tag="PC414_000919"
     CDS             complement(19200..19769)
                     /locus_tag="PC414_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF2463328.1"
                     /translation="MIIIYGYCRVSTKNQTDNNSLYQQQQEILNKYNDAKIIKETYTG
                     TTTNRPKFEKIISSLKKGDLLVATKLDRLARSTSEGIQLIEGLFAKEVSVHILNIGLL
                     ENTSMGKFFLTTLLAVAELERNMIIERTQLGKEFAKKKKDFKDGRPKKYSNQQIQYAL
                     GLLNSKPYREVVKMTGISKSTLIRAKKSI"
     gene            19972..20199
                     /locus_tag="PC414_000920"
     CDS             19972..20199
                     /locus_tag="PC414_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDF2463329.1"
                     /translation="MNSLLKEAIDELKNIHCGEVFLLKELFKGYEWNRLEMNIRLKLG
                     KLFLYEVVTNPNLNVEVINKNSAYQQMYKKK"
     gene            complement(20418..21161)
                     /locus_tag="PC414_000921"
     CDS             complement(20418..21161)
                     /locus_tag="PC414_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HDF2463330.1"
                     /translation="MENYQSTNTCTYNILYVQFNEDFERSKYFSYDVKYPVIYNIRNQ
                     STYVNPVILNNINNAVRMTVNNFKNGLQEEEREYNNAASQNSLPKQSYWVSTSYAVTF
                     NKNYVLSMILNLMGFANNYGPKYNTLNNYNIDLTTGKELLLNDIFRDGVDYINIITDY
                     IKSQINENREMYYSNVELVIPEDQAFYLTDNGIIIYFGVDEIAPESFGIPKFELKFSD
                     FGNFINLRFYCISPDIYVQSRGRAKHFMG"
     gene            21329..22201
                     /locus_tag="PC414_000922"
     CDS             21329..22201
                     /locus_tag="PC414_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF2463331.1"
                     /translation="MLLLADKWKDYELIDMGNGEKLERWGSYVLRRPDPQVVWPMESE
                     WALWKNPHGHYHRSNKGGGQWEHKKRYPEQWTINYKNLKFHIKPTGFKHTGLFPEQAA
                     NWDWMIEKIKKANRPIKVLNLFAYTGGATVACASAGAEVCHVDAAKGMVNWAKENIEL
                     SGLKNQTVRFIVDDVVKFVEREIRRGKKYDAIIMDPPSYGRGPKGEVWQIEEKLYSFV
                     DLCMGVLSDNPLFFLINSYTTGFSPIVLENVLSTTIGKKTNTGDIYGGELGIPASRDG
                     KILPCGIFGRWEKK"
     gene            22201..22866
                     /locus_tag="PC414_000923"
     CDS             22201..22866
                     /locus_tag="PC414_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDF2463332.1"
                     /translation="MIKVIYEDNHLLVVEKPVNILSQGDNTNDKDMVNLLKDYIKKKY
                     NKPGNVYIGLIHRLDRPVGGVMVFAKTSKAASRLSEQVRSKTFKKTYLAVVHGKIKSN
                     SDILKDFLYKNKKTNMVTVVKKSHKDAKEAELNYNVLDFKENLSLVQVNLKTGRSHQI
                     RVQFASRKHPLYGDQRYGKEINKIGQQISLWSNKIEICHPTTKEKMEFTCHPPDKYPW
                     NIF"
     gene            22887..23744
                     /locus_tag="PC414_000924"
     CDS             22887..23744
                     /locus_tag="PC414_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2463333.1"
                     /translation="MSKKFSIGEMSKLHKISIQTLRYYDQIDLFKPVHVDQESNYRYY
                     SIDQFSQLDIIKYLKYLGMPLKEIKSKLDENNEEILKLLTNKINIVDEKIKELELIKK
                     VLSTKKETMEKGMKVDEVGKIVRKHIPRRSILSIEYEEGLSSEETMELSRRQIANILE
                     DNISVFYGGVSGLVSIDKFLKEDKVIYDNSFVTVERDLFDIKSQKHITEISSGDFICM
                     TYKGPYKENYTAFKKLVDYVEYKRIPIENYIYEIPIIDPLSTNNEEELLTEIQIQVKK
                     REIDLRCLV"
     gene            complement(23900..26446)
                     /locus_tag="PC414_000925"
     CDS             complement(23900..26446)
                     /locus_tag="PC414_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2463334.1"
                     /translation="MNIFNKVALQGLKKNRTRTLVTIIGVVLSAAMITAVTTFGVSLM
                     NYMINGAIIKYGDWHVQFLDVDSSFVQEQRVNDEVANTATFENIGYATLEGNRNPDKP
                     YLFIAGFSKEAFDTLPITLISGRLPENSREILVSSKVATDGGVKFAVGDTISLDVGKR
                     MSENEKLGQSDPYTAGSEVFVPQDKKTYTVVGICRKPVFEESSAPGYTLITKEDKQVK
                     TDSLSLFVTLKNPHQIRSYTSSTKDHASIFNDNVLRFIGVSDNNLFNMFLYSVGGIVL
                     AIIMLGSIFLIYNSFNMSLNERTHQFGILSSVGATAKQLRNSVLFEGICIGAIGIPIG
                     VAIGIVSIGLVISVISKNFANILYVNVPLTLTLSIPAIIVAAVVSMVTILISAYIPAR
                     KAANTPVMECIRQTNEVKIESKSVKTSKLTQRVYGLEGVLAMKNFKRNKKRYRSIVLS
                     LVLSIVLFISTSALVADLKQMSNQRSEVTDQDIIFSTQGMKDNEMLKLYDKLKTANGV
                     YEGLYQKLMTYSCVAKVSELTDDYLNYEGSHSMSEMLELPVDMQFLDDRTYLNIIKSL
                     GLPAEEYTGQNAKLIAVSMVTKDNNIIDIFNSSSMNVSLTPETNGKLEIPERQNVNIS
                     FVKVVPPDSPPGIRSSDVKKASFRVLAPYSAKDKFEIPGVSTGIESLSFQSKNPTQSV
                     AEMEQIIESEGVTNEYNLYNMYGMMDENRNYIFIANVFAYTFIIMISLISIANVFNTI
                     STNIKLRRRELAMLRSVGMSEHDFQKMMNFECAFYGMRALFFGLPIAVISSWLIHKVM
                     VTDDIDFVIPWFSIGISIFSVLFIVFITMLYTINKIKKENIIDALRDDMA"
     gene            complement(26446..27132)
                     /locus_tag="PC414_000926"
     CDS             complement(26446..27132)
                     /locus_tag="PC414_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2463335.1"
                     /translation="MAFLKIEDLCKVYGKNENKVTALDHVSLTIEKGEFTAIIGSSGS
                     GKSTLLHSIAGVDVPTSGKIYLEGQDVYGQSNEKLAIFRRRQVGLIYQFHNLIPTLNV
                     VENITLPILMDKRKVNQERLNDLLELLGLKERKTHLPNQLSGGQQQRVAIGRALMNAP
                     AVMLADEPTGSLDSKNGQEIIQLLKESHSKYHQTLIIVTHDENIALQADRIICISDGK
                     VVRDERKVNR"
     gene            complement(27215..28204)
                     /locus_tag="PC414_000927"
     CDS             complement(27215..28204)
                     /locus_tag="PC414_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2463336.1"
                     /translation="MFRNREFRKFAILFLLITILTVALGFAISIMTGILSIVSATTFG
                     IAFFVFTKNRYKSIAQISEQINLVLHNANHLYIAESDEGELSILQSEITKMTLRIREQ
                     NYALKKEKEHLADSLADIAHQLRTPLTSVTLILSLLENTSDEDERKELIRETEELLIR
                     MDWLITSLLKLSRLDAGIVVFQKEQIDVNNLISSALHQLLIPMELHNITLHIDIPKGV
                     RILGDLNWLSEAIQNIFKNCMESVGDNGKIDIICEDNFLFTQLTIHDNGAGFKKEDLP
                     CLFNRFYRGKNSSTAGYGIGLALCKTIIMRQGGTITAQNHPQGGAIFVIRFPK"
     gene            complement(28191..28877)
                     /locus_tag="PC414_000928"
     CDS             complement(28191..28877)
                     /locus_tag="PC414_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2463337.1"
                     /translation="MKRIFLVEDDKAIAKNLILLLNSEGFIVTHVSTRSEALVEIAKN
                     KFDLALIDISLPDGNGFTICTEIKEKQDIPVIFLTASGDESSVVTGLNMGADDYITKP
                     FRPRELIARIRTALRKSGRFGSTFEICGLYVDTATGVVKKNGNEVFLSALEYRLLLVF
                     ISNPKSIITRVRLLDELWDAAGEFVNDNTLTVYIKRLREKIEDDPSSPQIILTVRGTG
                     YRLGGSYVSK"
     gene            complement(29377..32583)
                     /gene="carB"
                     /locus_tag="PC414_000929"
     CDS             complement(29377..32583)
                     /gene="carB"
                     /locus_tag="PC414_000929"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDF2463338.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQALKEEGIEV
                     VLVNSNPATIMTDKEIADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     HDAGILDKYNVKVIGTSIESIKKGEDRDLFREVMKEINQPVIVSDIVTNLEAGLEFAN
                     KIGYPVVVRPAYTLGGTGGGIADTEEELREILSHGLQLSPVGQVLLEKSIKGWKEIEY
                     EVMRDGNGNCITVCNMENVDPVGVHTGDSIVVAPSQTLSDEEYQLLRKASIDIINAIE
                     VQGGCNVQIALNPHSLEYAIIEINPRVSRSSALASKATGYPIAKVAAKIALGYTLDEI
                     ENAVTKKTYACFEPTLDYVVVKIPKWPFDKFRHANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHAPSEGRTLEELKARVVVPDDERLFDLAEMIRRGYKVEMISE
                     ITGVDKWFINKFKWIVEQEEKLKVLKIEDLDKEYLHELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIQPVYKMVDTCGGEFEALSPYYYSTYEQYDEVVVSDKRKVVVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMGIETIIVNNNPETVSTDFDTSDKLYFEPLTEEEVLNI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFRDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWTTNEGVEIAKELGYPVLVRPSYVLGGQGMEITYNEEKLIQYLDDAFDRD
                     HKNPVLIDKYLTGREIEVDAICDKEDILIPGIMEHLERAGVHSGDSTTMYPSQNISDE
                     IKEKIVEYTKKIALDLNVLGMVNIQFIEFQNELYIIEVNPRASRTVPYISKVSGVPIV
                     DLATKCMLGAKLKDLGYGTGVYKEPKLVSVKVPVFSMSKLSKVEVSLGPEMKSTGEVL
                     GVGENLEEALYKGFLAAGRHMSDERGVVLATVNNHDKDEFIEIAKDMKELGYTFVATE
                     GTAKSLRENGIEADIVNRIEEPRPNILDAIRNKQVDIVINTPTKGNDSTRDGFKMRRT
                     AIEFSTEIMTSLDTLKALVEVKKKHLNKDKLKVYNIAE"
     gene            complement(32776..33825)
                     /gene="carA"
                     /locus_tag="PC414_000930"
     CDS             complement(32776..33825)
                     /gene="carA"
                     /locus_tag="PC414_000930"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002845470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDF2463339.1"
                     /translation="MKARLILEDGTVFIGKAFGYLEESVGEVVFNTSMIGYGEVLTDP
                     SYYGQIVTMTYPLVGNYGINLSSAESEKVQVKGFIVREKSDSPSNFRCEIDIDQYLKQ
                     NKVIGLEGIDTRALTKILRNNGTMKGIITLEDSKLEDVKHKLDKFSNTEAVRTVTRKE
                     VEHIKGNGPKVAVMDFGVKRNILRSFIARGCDITIFPATTSPEDVLSINPDLIFLSNG
                     PGDPEDLEDVIENIKALIGKKPIVGICLGHQLLALALGGKTAKLKFGHRGGNHPVKDL
                     EEGKVFITSQNHGYYVSEVPEQMKVTHINLNDNTVEGMRHEKLDVYSVQYHPEACPGP
                     KDNDYIFDKFLELVK"
     gene            complement(34082..37288)
                     /gene="carB"
                     /locus_tag="PC414_000931"
     CDS             complement(34082..37288)
                     /gene="carB"
                     /locus_tag="PC414_000931"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDF2463340.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQSLKEEGIEV
                     VLINSNPATIMTDKEVADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     YEKGILDKYGVKIIGTSVESIKKGEDRDIFREVMKEINQPVIVSDIVTDLQAGLDYAL
                     TIGYPVVVRPAYTLGGTGGGIADNEEELREILSHGLQLSPVGQVLIEKSIKGWKEIEY
                     EVIRDSKGNCIVVCNMENIDPVGVHTGDSIVVAPTQTLSNKECAMLKKASLDILNAVE
                     VQGGCNVQFALNPHSFEYAVIEINPRVSRSSALASKATGYPIAKVSSKIALGYTLDEI
                     ENAVTKKTYACFEPTIDYVVAKIPKWPFDKFKKANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHTPSEERSIEELKKRVVVPDDERLFDLAEMIRRGYKVEMIEQ
                     ITGIDKWFINKFKWIVEQEEKLKVMKIEDLTKDYLLELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIKPAYKMVDTCAGEIDAISPYYYSTYEQYDEVVVSDKKKVIVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMDIETIIINNNPETVSTDFDTSDKLYFEPLTEEEVLSI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFDDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWSLNEGIEVANELGYPVLVRPSYVLGGQGMEITYNEEKLSQYLQDAFDRD
                     HKNPVLIDKYLTGREIEVDAICDKEDILIPGIMEHLERAGVHSGDSTTMYPSQNISDE
                     IKEKIVEYTKKIALDLNVLGMVNIQFIEFQNELYIIEVNPRASRTVPYISKVSGVPIV
                     DLATKCMLGAKLKDLGYGTGVYKEPKLVSVKVPVFSMSKLSKVEVSLGPEMKSTGEVL
                     GVGENLEEALYKGFLAAGRHMSDERGVVLATVNNHDKDEFIEIAKDMKELGYTFVATE
                     GTANSLRENGIEADIVNRVEESRPNILDAIRNKQVDIVINTPTKGNDSTRDGFKIRRT
                     AIEFSTEIMTSLDTLKALVEVKKKHLNKDELKVYNIAE"
     gene            complement(37341..38384)
                     /gene="carA"
                     /locus_tag="PC414_000932"
     CDS             complement(37341..38384)
                     /gene="carA"
                     /locus_tag="PC414_000932"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDF2463341.1"
                     /translation="MKGKLILEDGTVFEGKAFGYLKEAIGEVVFNTSMAGYGELLTDA
                     TYYGQIVTMTYPLIGNYGINLEDVESNKVQVKGLIVREKSDNPNNFRCEMDIDTYLKQ
                     NKVIGLEGIDTRALTKIIRNNGTKRGIITLENVSLQDVQSKLEEHCNKDSVKNVTRKE
                     IENIKGTGAKVVVIDFGVKNSVLESLKACNCDITIVPAMTTVEEILNINPDLILLSNG
                     PGNPEDINDIVENIKKLIGKKPLTGIGLGHQILALALGGKTSKLVFGHRGGNHPVKCL
                     ETGKVFITSQNHGYNVSEMPENTEVTHINLNDNTIEGMRHKELPIYSVQYIPEYTTKE
                     DLDCIFNKFLALI"
     gene            complement(38410..39126)
                     /gene="pyrF"
                     /locus_tag="PC414_000933"
     CDS             complement(38410..39126)
                     /gene="pyrF"
                     /locus_tag="PC414_000933"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HDF2463342.1"
                     /translation="MINGKEKLIVAIDTDEFDKAKELIDRLEDSVDIFKVGLEQYVAT
                     KGKTIDYLKEKGKKIFLDLKFHDIPNTMKSAVKAAVRDNVWLMTIHVSDMEGMRQCAL
                     IAREEAERLNIEKPLVVGVTVLTSLSNQDLQDIGCNMTTEELAIKRAKLAKESGIDGV
                     VCSAQEVDKIVEACGTDFITVCPGIRPKSAEVGDQKRVVTPSDAIKKGAHYLVVGRPI
                     TKAENPRESAINIVREIENA"
     gene            complement(39502..40137)
                     /locus_tag="PC414_000934"
     CDS             complement(39502..40137)
                     /locus_tag="PC414_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HDF2463343.1"
                     /translation="MYKCGKCNLRGCSNGNMDKLMKICPTREIELQQKAKQLYEEEEN
                     RKIAYNAGLVEAEGYCQYTRLKEIIEFAKKCGYKKIGIAFCIGLKREMQTVQKIFEHH
                     GFEVFSVICKNGAMPKSTIGLEASQTISKCSDEVMCNPIGQALFLNEAKVDLNVILGL
                     CVGHDTLAMKYMEAPITTLVVKDRVTGHNPVAAIYNAESYFEKKLFDKDEE"
     gene            complement(40161..41075)
                     /locus_tag="PC414_000935"
     CDS             complement(40161..41075)
                     /locus_tag="PC414_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463344.1"
                     /translation="MELYEAIFYRKSVRNFSSKKIKKPLMEEIKRSCKNLEYLNEDLN
                     IKAHVVDRGHIIHFLMGKECKVKAPHYIVVTSDKGEDYLQNIGFATEGVVLRLTTLGI
                     ATCWLEGNLKREDILEFVDIGNKNFEEDEEDDLDYLAGIKTEEDDENKIENPYIIIAF
                     GYPAKNEELFRTRYARPDRKPVKDMINKINRNRKWKDILELTRRAPSVKNSQPWYFHK
                     DERGLHLFEKRPKKHCEDMNKVSLGVALRHFDIACIKNKIDVSYEKLPIRNKIGKSYF
                     ITVVEHVKPEEETQEENVTLEKEESQDE"
     gene            41676..42905
                     /locus_tag="PC414_000936"
     CDS             41676..42905
                     /locus_tag="PC414_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDF2463345.1"
                     /translation="MNLNRNLEKYAELAIKVGVNIQPGQILLIKSPIECADFARNALK
                     EAYKCGAKNVYIEWSDEESTLIKYLYAPDEAFHEFPKWTAEQYVDIAKEGGAFLSVYA
                     QNPDLLKDVDPERVANFQKASGKALKEWRSYTLTDKCKWSIVSVPTKDWAKKVFPNLS
                     EDKAIEKLWDAIFKCSRVDGQDPIKAWEEHNENLKSKMDFLNKNNFKTLKYKSSKTDL
                     TLDLPKGHVWLSGASKDPNGISFNPNIPTEEIFGMPHKFKVNGTVYSTKPLVYGGNII
                     DNFFLTFKDGKIIDFSAEKGLDTLEKLIETDEGSHYLGEVALVPYNSPISNTDIIFYN
                     TLYDENASCHFAIGSAYKTCLEGGNNLKDEELDEHGVNDSLTHVDFMIGSADMDIIGE
                     TYDGKQIQIFKNGNWAF"
     gene            complement(43110..45491)
                     /locus_tag="PC414_000937"
     CDS             complement(43110..45491)
                     /locus_tag="PC414_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HDF2463346.1"
                     /translation="MEIRQYLKNNILIFDGAMGTMLQQKGLKLGENPEVFGLQNPDKL
                     IEIHTAYLEAGSNVILTNTFGCNELKLDSKYTVEEVIDNAVLVARKAIENVDNTKPRY
                     VALDIGPIGEMLEPMGTLSFDKAYEIFKRQVLQGVKSGVDVIVIETMMDLYEAKVAVL
                     AAKENSDLPIFCTMTFDEGGRSFTGCMPECMVATIEGLGVDAIGVNCSLGPKQLLPIV
                     EKIASRATVPVMVQANAGLPNIVDGEAIYDVDAKEFFEGVKKFVEVGATIIGGCCGTN
                     PSFIKEISENINSVTKGCIEKIDKCVVCSPSKFVEVQSPTVVGERLNPTGRKSLQEAL
                     KNENVDYAINLGLEQVNAGAQILGVNVGLPEIDEKKLMPKLIREIQAVVDTPLQVDSS
                     NVEALEQGLRYYNGRTIVNSVNGKEESLESILPIVKKYGSCVVGLTLDEKGIPKKAEE
                     RFEIAKRIVNRAVSYGIKPKDIFIDCLSLTVSAQQEEAIETIKAITMVKTLGVKTILG
                     VSNISFGLPNRKALNASFLTLALGAGLDLAIINPNEYSMMEAINSFKILNNTDKGCIN
                     YINQYSNINNSKKSDSSTKIDKDLPLDILVERGLKDEAKNVTLNLLKEKDENYILDEI
                     LIPALDKVGKRYDSGDIFLPQLIQSAETVKVSLNTIKETLLSKSSNNVSKGKIIVATV
                     KGDIHDIGKNIVKIMLENYGYEVIDLGKDVPIEEVVEVAKKRNIKLVGLSALMTTTVQ
                     SMKDTIQALRDNEINAKVFVGGAVLTEEYAEEMGADYYSKDAKSAVEIAKLNF"
     gene            complement(45463..46188)
                     /locus_tag="PC414_000938"
     CDS             complement(45463..46188)
                     /locus_tag="PC414_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vitamin B12 dependent methionine synthase,
                     activation domain protein"
                     /protein_id="HDF2463347.1"
                     /translation="MENNLIGREDISINQSEVLRYLQYRGQEIDKDLKYTIDECVAIT
                     KNKINPRYLSRIYPIKINKDNVVELEGTNLTLESKDVYELLKECNECVIMAATIGIDI
                     EKEIRRHSYSNLTKGLIIDSCGTTAIEEVCDIVQNNLEKVFLKQDKFITMRYSPGYGD
                     LPIEKNIDILNVLNAQKQIGLTITSNGIMIPRKSVVAIIGISNTRINKSKKEKSCENC
                     KNYRNCTYRKGANSCGNKTISEK"
     gene            complement(46178..46633)
                     /locus_tag="PC414_000939"
     CDS             complement(46178..46633)
                     /locus_tag="PC414_000939"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HDF2463348.1"
                     /translation="MEKVESFKLDHTKVKAPFVRKCSVLDGVKGDKVTKFDLRFLQPN
                     VESFGTAAMHGLEHLLATYLRDTLDGVIDLSPMGCRTGFYLILWGDVDAKTVKIGLEE
                     ALKKVLESDKMPAATAIECGNYRDLSLFGAKEYAKDVLDKGFSLNIYGE"
     gene            complement(46915..48096)
                     /locus_tag="PC414_000940"
     CDS             complement(46915..48096)
                     /locus_tag="PC414_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF2463349.1"
                     /translation="MKNKSLLNHMFRPILDLIISCALTSMLILTSMYIIKSLYVNSDM
                     SVVNQLVYQARDFRSLIGYDLFYPFLFTITSLAIYSMISYKRNKQIVDFIRKTNRKNQ
                     KLEIIKSAVNLMDEGNLEKSIDIEYDLDILKDKEKDDIDELAHKINNIVHQLRNITIE
                     ERQAQQTKTDLITNVSHDLRTPLTSIMGYLGVIEEGKYKDEVQMMYYVDIAYEKSKNL
                     NVLINDLFELTKMQNNTIKLNKIEINLVELLSQVVSQFEIYFKQEFMVSRINFNEEKL
                     IVKADPNKLVRAFENLITNAVKYGKDGHYVDIVTEKKEDMAVVKVINYGEPIPVLDLP
                     NIFDRFYRVEKSRNRNVGGSGLGLAITKNIVNLHDGEITVSSDKYQTVFEVQLPIIQS
                     A"
     gene            complement(48086..48778)
                     /locus_tag="PC414_000941"
     CDS             complement(48086..48778)
                     /locus_tag="PC414_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2463350.1"
                     /translation="MNNTVLVVDDEKEIRDLIEIYLLNSGYNVIKAEDGKEALDILKK
                     ENIHLMILDIMMPKMDGIEVCRKVRESLNIPILMLSAKSEDMDKIQGIMTGADDYLTK
                     PFNPLELTVRVKSLIRRAYYFSGANNEDKDVIKVGVVIIDKEKHSVTVAGNEIILTSR
                     EFDILYLLANNKGRVYSTEEIFEKVWKEKYYQSNNTVMVHMSRIRDKIEKYTDGEKII
                     HTVWGVGYKIEK"
     gene            complement(48912..49628)
                     /locus_tag="PC414_000942"
     CDS             complement(48912..49628)
                     /locus_tag="PC414_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDF2463351.1"
                     /translation="MKKFKLIGAIILVVFISVFISKDRLLKKDKDPIVAAQHDSEQDV
                     EANSQNKNHSNSISNILLVNKTNGISKNYTPENITKVNIPFVEEATEEEKQMAGEPAK
                     AVEDLVKQANSEGIQFLGSSAYRSYDTQLDTYTRRVKSQGREKADAYVAKPGYSEHQT
                     GLCIDLTNPERWFVGSTKEAKWLAENAHKFGFIIRYPEGKEDITGTAYEPWHIRYVGK
                     DAAEEIYSKGLTLEEYLQNK"
     gene            49753..50487
                     /locus_tag="PC414_000943"
     CDS             49753..50487
                     /locus_tag="PC414_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HDF2463352.1"
                     /translation="MSIQKLLSKARKAIQDFDMIQENDKIAVGLSGGKDSLTLLHILK
                     SYQRFSPQNFELIAITLNTGGVDNSPLDKLCKEINVPFYEFQTDIKEIVFDIRQEKNP
                     CALCANLRRGALNDNAKKLGCNKVALGHHKDDAIETFLMSMFYEGRVNCFSPKTYLDR
                     QDLTVIRPMVYIEEYMTKKISKDSNYPIITNPCPANGHTRREYIKNLIANLNKEMPDF
                     KRNVFGALNNSEKLFIWDKEKIKNFK"
BASE COUNT        15944 a         9264 c         5679 g        19673 t
ORIGIN      
        1 ttaaattaat acatgcaaat ataatttgct tattaattta atattatact ttcagtttta
       61 gtatttaatc cacatcagat aataatctta aaatgaccaa ggtcttaaaa ctatttatct
      121 ttatcagatt tatcttctaa atttttattt atatcaataa cattgtttct ttcttcttca
      181 tctttttcta attttatagt taattgttct tcaacttctt tagaagaatt ttcctctgta
      241 actgcatttt caatttcatt cagaggtaac tctttattaa atgcttttac aaattgttct
      301 gcatctaaag tttcatatat aagtaaagct tgtgctacat actctaatct atccatattt
      361 tcttgtaata tacttcttgt cttatcatat gcaccatcaa ctatacgctt tgtttcttgg
      421 tctatctcaa aagcaacttc ttcagaataa tttctcttac ttgaaaaact attacctagg
      481 aatacttcat catcactgtc aaatgacatt ggaccaagtt tagaactcat tccatacttt
      541 gttaccatac ttcttgcagt agctgtaact ctttctaagt catttgaagc acctgtagat
      601 acatccttaa gtactagctc ttctgctact ctaccaccaa gtaaaactat aatattttct
      661 ttcatttcat tttttgtagc atagaactta tcttcaacag gaagttgcat tgtaaaccct
      721 ccagctcttc ctcttggaac tattgtaact tgatgaacag gacttacttc ttcaagtaca
      781 tgggcacaaa ctgcatgccc tccttcatgg tatgctgtaa gtcttctttc tttctcactt
      841 attactttcg attttttagc cacacctgct ataacctttg ttatagcttc ttctatagtt
      901 tccatcttaa ttttcttttc tctttttcta gctgttaata ttgcagcctc attcattaag
      961 ttctctatat cagcaggagt aaatcctggt gttcttctag ccaagacatc catttttaca
     1021 tcatcactta atggtttatt tcttgagtga actttaaata tagcctctct accttttaca
     1081 tctggtgtac caacaacaac ttgtctgtca aatcttcctg gtctaagtaa agctgggtca
     1141 agtatatctg gtctatttgt agctgccatg attattatac cttgatttac cccaaatcca
     1201 tccatttcaa ctagaagttg attaagagtt tgctctcttt catcatgacc tccaccaaga
     1261 cctgcacctc tttttctacc aactgcatca atttcatcta tgaatatgat agctggagca
     1321 cttttcttag cctgttcaaa taaatctctt actcttgaag caccaacccc aacaaacatc
     1381 tcaacgaaat cagaaccact tatactgaaa aagggaactc ctgcttctcc tgcaactgct
     1441 cttgatagat atgttttacc agttccagga ggtcctacca taagcattcc ttttggtatt
     1501 cttgcaccca attcaatata tttcttagga ttctttaaga aatctactac ctcctgcaaa
     1561 tcttcttttt cctcatctag tccagctaca tctttaaatg taactctagt tttttcatca
     1621 tctttatgaa ccttagcttt tgatttgcca aaactcataa cctttccgcc tccaccttga
     1681 gactgattca taaacacgaa ccaaataatc accatgaaga atattaacag taatgtaggc
     1741 aacatttcga caaaccaagg tgtagatggc ttagcttcac caccaaaagt tagtttccca
     1801 gccttagctt ggtctaacac ctcatctgca agtttatttc ccatcacttc tgttggaaca
     1861 tatgacttaa actttgtatt tgtatctttt atagtacctt ctacagaagt ttgatttaca
     1921 aagtataatc ttgatatatt ctcatctgtt aattctctat atacttcaga gaattttaaa
     1981 tcttttattt tttctgtagg cttacctgag aattgaacta tacctactat aattataaaa
     2041 acaagtagat aaaaacctgc cccttttagc aacttattca atatagaagc cctcctttca
     2101 tacattgctt tgccctataa tttgttaaaa ttaattttta atacttcttt tgtgctttca
     2161 tcaattttat aattttcact gattttataa tctccaacac atattattcc ttcaccatct
     2221 gtgattatag gaattttaca tctttcttct cttgggattt ttaaatctat gaataaatct
     2281 ttcacttttt tacttcccat agcaagcttt atcttatcac cttgccttct gcttcttatt
     2341 actatacccc cttttacctt attaaaatca aaccctttag atgaattgtc taatttcata
     2401 ctcttatacc tatctatgct cattacttgg gtctcaataa tttgttttaa ttcttttatt
     2461 ttaataaacc cattactagg tacattgtaa taaaattcaa tttcctcatt aacaatttcc
     2521 tcattcgtta atattatact gtcttttttt ctataaacaa atatatttct tggaagagtt
     2581 aacattttat ttacttttga attatcttct aaagacatta tatcttctat atgtctttgg
     2641 tcaacaaaat tagtatctcc tagtatatgt tttatggaat ttctaagaac tctaactttg
     2701 atagcatcat gtaagcaaac aaaatcgtct aaatttatct ctacaaagcc tttttctttt
     2761 atatttgaaa cttctctaaa tttagcttct gcctctttct caatataatc gttatcactt
     2821 tttaaactat tgctcatcct cactatagat tctattacat ttgaattaaa attatccttc
     2881 atatatggta aaaggtctaa ccttatctta tttctagtgt atatattttc taaatttgtt
     2941 ttatctatcc tcggatttaa attataagct tcacaatact cctctatctc atttctttcc
     3001 acgtctaata tgggtctaat tatgcaatta tctctaatat aatcaattcc ctttaagcct
     3061 tttaaacctg ttcctctcat gatacgcatc ataaccgttt cggcttggtc attgagatta
     3121 tgacctattg ctattttatt ggctttaatc ttatccttaa tttcataaaa catttcatat
     3181 cttagcttcc ttgctccttc ttctaatgat aatccctcat tttcacaata ttttggtaca
     3241 ttaatagact ttacaaagaa tataattccc atatcctcac aaagtttaga tacatataat
     3301 gcatcttttt gagcttctat ccctcttatt tgatggttta aatgtgctgc atatatttct
     3361 atattaaaat ctttttttaa tctatttagt acatgcaata aacaaactga atcaggtcct
     3421 ccagaaagac ctaaaactat tttatcgcct ttttgtatta aattatgttt atttatagta
     3481 cttagtacct tatcaaatat catatttatc cctcttacac ttagttttat tcacttttaa
     3541 gtatactttc tataaaaaaa cgctaaaccc ttgtatcaat tttaagaaat taaagtttaa
     3601 tataaatata ataataccaa ataaattaaa taaaaggtag ttttttcact acctttataa
     3661 aatttctgga aaatagccat agttattatt tagtacctat gtatatatct attttattcc
     3721 tacctaaaaa taaatcttgt acctttatat tatagtctat tttcaatttt atgtgttccc
     3781 cttcttgtat atttatatct aattctcttg tatcaatatc aattaataaa gtaccttgtg
     3841 gggtcacata ctttgtagta ctactttctc cacatttaaa cagcattgtt gagttaatag
     3901 caccaaactt tttgatacta acttgatttt taagtatctt tatagtatta gttatttcta
     3961 tatcttcttc tttttccttg taaataacat atattccatc atttttataa aaaattttac
     4021 caaaggcagt catctctatg gtgtccatat ttcctttttc atcatattgt ctagtattta
     4081 tacttatttt tacactcaac gatttttcct ccatagattc ttcatataaa ctagtatttt
     4141 tgtatttcta ctttttgaat cttatcaatt tttttcttta agaactctct tgctatatct
     4201 gaaaattttt caggtatatc tgatacataa tattgataag ttccatgaat ttctgtattg
     4261 tttataatat tttgcacctc taaaatcttt tttaaatcct ttgcagtctc tttagctgga
     4321 ttaacaagtt ttatatgctc tccaacttct tcacctattg ttctctttaa aataggataa
     4381 tgagtacatc ctaacactaa agaatctata tttttttctt tcaattcttg taaatatatc
     4441 tttgctgtca gtttagccac ttctgtattt gcccatcctt cttcaacaat aggcacaaat
     4501 aaaggacatg ctttattaac aatttctata ctctcatcta ttttagatat ttccaagttg
     4561 tatgcttttg aacttatagt tccttcagtt cctattattc ctacaatttt gttttttgta
     4621 gaacttacag ctgttctagc tcctgcttct ataactccaa ttatcggtat atcatatttt
     4681 tcttgagctt cttttaagct tcttgcagta gcagtgttac atgctataac tatagctttt
     4741 acaccttttg tttttaaaaa gtttatagct tgaaaagtat atttaattat agtttctttg
     4801 gatcttgaac catatggtat tctggcagta tctccaaagt atattatatc ctcatttggt
     4861 aatatcttca ttatctcttt tagaactgtt aatccaccta atcccgaatc aaaaactcct
     4921 attggtttat tgttcatctt tatacaacac ctactttaca tttttgtgtt taacaacaac
     4981 ttatttatcg ctaaaattta tatttatatt cataattaat atattattac ctaaatgatt
     5041 attatagttt attttacaca aaaaataaag gagctatctt aaattagaaa ataatttcta
     5101 caaaaagaca actcctttca ctacttaaaa atttaaattt attttgcaaa cacatgattt
     5161 cctattctca tataaggatt caatctattt atccatttgc aagtggctat ttctggattc
     5221 caaaaatata ctgctttatt agttgggtca gttccataaa gagcttcctg agctgcttta
     5281 cgtgctgagt ctgttggtgc catctttatt gaaccatcaa gcacaactga gaaagcattt
     5341 ttttgatata tgacttcttc tattgtgtct ggaaatctag ggtccaatac tctatttatt
     5401 actacagctg ctacagcaac ctgtccctca tagctttctc ctctagcttc tcctgcaacc
     5461 aacttagata ataacatcaa gtcattatca gataaattta ttacttcttt ctttgctata
     5521 tttttttctt gtttgtttac tttcttaata ttttctgttg gttcatatgc aaatacctct
     5581 ttaataggca taaatataaa agtcaaagct aatagtgtag ccatcagttt actccacctg
     5641 tccatgatta aaaccactcc ttacattctt atttttaaaa ttttttctct aaatccttga
     5701 ctacttcaag tactttaaac ttaattttta ctctacttgt tttttttggt gtatcttgtt
     5761 ttattaactt atattcttta tcagttaaaa cttccttcaa cttatcatct gtagatttat
     5821 ctatcactcc attagattca tcaacaaaac atagtggagg gaacataaca caccaccagt
     5881 tttttccttc acctttccca ataataatct ttaatgcttt atattcacca gctggaagaa
     5941 ctatattaga atattgttta gtagggaaat tgctatattg tatacctacc tttacactgt
     6001 aattatatcc atttttaaga ataatatttt tacttatttc ttccagacta ctatattcct
     6061 tttttataat agcttcactt tcttttatac tttttgaatt ttgaagcttt ggctgtaaat
     6121 attttattac ttcatctcta acttttagtt taagttcttg gtcttcatct gtattgctgt
     6181 ttgctataac atgaaatctt attaatttat ctttataatc ttttgatata ttgtctactt
     6241 tttttacctc accatttatt actattgtca ttattgatat gactgatata agacttaata
     6301 ttaatagtcc caatctaatc ttaatttttc tcattgaaaa ttccccctca agataaattt
     6361 atatcatcaa ttatgtccaa ttaaatttaa tttattcata atatttttta atactaataa
     6421 aaaaagctat cttaaaatga ttacaatcat tttaaaatag ctttttaaac cttatatttt
     6481 ataagtcttt actaatcata gccttatcta tcataaactg ctctgctttt tctatatgct
     6541 ctgtagcata cttcttggat agctcactat cacctttaat tatagcatca agtaaatatt
     6601 tatgctcttc aacaatatta tgtgatgtat cataagcaga catataggta actctaaatc
     6661 tatatacttg ttcccacaat gagtttatta attggattaa ttttttatta ttagttgctt
     6721 taaatataca ttcatgaaat tctacatcaa gttttaataa cgcatctaca tcggattcca
     6781 aagtgctttt tttaaattct tctgatatat ctttaagctt tttaatatct tcatcactaa
     6841 ttctttcagc ggcaagatat gctgctaagc cttctaggct tgctcttact tctaatacat
     6901 ccataatatc ctttaatgac atatttgcca catacgcacc ttttctaggc aacataacga
     6961 ctaaaccttc taattcaagc tttcttattg cctctctcac tggagtccta cttactccta
     7021 attgttctgc tagttgcact tccattagcc tttgaccagg ctttaatttt ccttctaaga
     7081 ttgcttctct taaattctca aagactacat ctcttaatgg cttgtaatta tccaagttca
     7141 gtttagttaa attctccaca aatttcaacc cctttttgac tgctattaac aacatacacc
     7201 tgtttatatt tttttaataa ttcttttttt ccaattagag catcttcttt atttttaaat
     7261 agtccaaaaa ctgtaggacc acttccactc atcatagaac ctaaagcatt atttttcatc
     7321 attacttgtt ttatatcact tatttctttg tgtttaccta tagtaacatt ttcaagaata
     7381 tttaccatgc tttctgatac agcttttata tcttcagatt ttaaacactc tatcaaatat
     7441 ttattatttg gtcttttttt aatattattc aaatctaatc cttgataaac ttctttagta
     7501 gaaacgaata agtctggttt gcatatcaat atatttgtat cacatggtaa acctttgata
     7561 tttgttagct tttctccaat accttgagct aaggcaggtc ttcctgatat acaaaaagga
     7621 acatctgcgc ccaaattaag tcctatttct tttaattcat cttcagataa tctaagttcc
     7681 cataggtgat ttaaacctac taatactgcg gctgcattag aacttcctcc agccatccct
     7741 gctgctacag gaatattttt ttctatgaat atttctacac ctttttttat ataaaattta
     7801 tttttaagta ttttggcagc tttataaaca atattatctt catctaaagg aatatctaaa
     7861 ctagtgcttt ttacctttat ttcatcttcg tctaattctt ttatctttac tatatcgtat
     7921 aaatctatcg tttgcattat catttcaaca aaatgataac cgtcttgcct ttttcccaag
     7981 acatcaatgg ataaatttat ttttgctcta ctctttagtc ttataaaatc cattctatcc
     8041 tccttgttta aagaataatg catcactaac aataatatta tactacaaaa aataaaataa
     8101 tagggtatta taatacccta ttatacttaa ttactttaaa ttttttaata ttaatctact
     8161 aatacgactt ttttttctaa aataagacac ttcccaggct taattggttc atcaagtttt
     8221 atatcattaa gttctgcaat ttcattttct gttgtattgt atttttttgc tatattccag
     8281 aaagtatctc cttctttgca tatatatacg attatgctag gagctttaga taaatcatat
     8341 acaccttggt cttctccctt gataataaaa ttttctgctt tcttatctaa tgcttcagta
     8401 aatctcttaa ttttgataat caagtctatc tggtccctat tcaaatcaac ttcgacttta
     8461 tcaatacatg ctgtattaaa cacagatgct gtatcagtta gattatccat ttctatatca
     8521 tgttcaaatg gaatttcctc actaatttta taaaccaatt ttaaaccttc aactgggaca
     8581 aataaaattt caacttttat gattccttgt ataatgcttt tatttccttc aatataacta
     8641 ttttctatag acatggttgg acaaacgctt acaatatctt ttatttgtat atcatcatta
     8701 tcatttctta tgccttctct aaccataaat gtttcttcac tacgtcttaa agtcttattt
     8761 aattgaatag gtttgtggtc aaattttatt atcttttgag gcgaatatgc atcttgtaat
     8821 acttctcttg taacttcatc tgtaactttt accttacaac aagccataca atctatttct
     8881 aaaagacctg tgttgctttc actattttgc ttgaaaatat gattaaaatc tgacatagac
     8941 aacaatactt cttcagtcat accatcacta actccaggga cttctacaaa ttgagtaaac
     9001 tcaataccta ctctatccaa ctctacaagt tcaccctcat atgtacaagc taacggattt
     9061 atttctaata ctccaccaat aataacttta ttgtcagtca ctctactttc ttttacttta
     9121 acacatggat ttaaacttat tatagattga atttcctctg tgtttattgt tatagtatct
     9181 cttatagaac tttctgcttt ttctattcca actatatctt gaaaacaaat ttcctttctg
     9241 tgcttttgta tgcattccac ttgcgccaca tcttttacaa tgtctaacct ttgcttttca
     9301 aacaaacttc cccttatatt cataagagct cctactttaa tctttctctc attcattata
     9361 gtacaatcca tatgctctac ttctgaaaat aacatgtatt ccatatcttg aataacattg
     9421 tctttttcta ttacttcgtt tatatctact tttccgtcta cattagaaat agtattttta
     9481 tcatctgcaa tatagattac attataatta aaactacctc tgcaaagtat cttcccatct
     9541 gccatttcaa tttttttaag tgatatgtat ccttctgttt ttacaatttc atatacatct
     9601 aattttttgt ctggcacaac tgcctccgct tcaatgaatg tttgaaattt tccaaagtct
     9661 attctattgt caactttaat tacatcttta attaattcca tagtctacct ccttaagtat
     9721 tataaaaata tcacactata tttatattta aggagcaaaa aaaaaattac acctattttt
     9781 aaataggtat aattttaact tatttgtaat ttatcttgat ctctaaaaac ttgcaacttt
     9841 acattagatg ttaataaatc tgagtaacta taagatactc taggatatcc attactttca
     9901 tcatctaact ttataacaaa cacgcttgga taaacttttt ctagtatacc ctcttttgta
     9961 ataatctgct ttcttccttt attagctttc aacaatattt tctttcctat atgtctctct
    10021 aagcttactc ttatcttatc tagagtttga acagtagcca caatatcacc ttctttaacg
    10081 tttatatgtt tacataatat cacatataaa catttttgtc aaatgtttaa actaattatt
    10141 ttatatattt tttgcaaaaa tgtcaatact atttttgaag atatacagta taattatgtt
    10201 aatttttgct aatgtaatat tgtttttggc attgcccatc taaattttcc tcttgtttca
    10261 gaaccaccaa ttcccttaac acctgtaata gtctgctcta taacctcatc aataggaact
    10321 actacatcaa ttcttgaaca agcaattttc tccactataa aaacagggtc taatgcatgt
    10381 atcattattc ttccaggcga acttgcatag tttgctcctg cacttattat tttttcataa
    10441 tttgattggc atgctcctgc gaatatgaca agattatcca aatttggttg ccatttacgt
    10501 gcttctttta ctgttttaac aaaatttgaa gagtttctat agttactcat atcttctata
    10561 tctcctcttt ttattgtcat tgcatcatga cctgttatta ctagtatatc cggattatat
    10621 ttttctagta gtgctcttac ttctttatac tgattttgct ctgaaacagc tacacctact
    10681 gcaggtattc caagttgagc atatacatcc aagcaaattt ttaaatattc tttatctcca
    10741 tctatctgta gtacttttcc aggcattcca tatgtgttag gatttaattg aagttttgga
    10801 actgctctag tcattctttt ttgtctttct ttagcttttc ttactgactt atataataaa
    10861 ttttctacat ttttatctat taatatatct tttatatcag gtgcttttac taattctagg
    10921 tcatcaatat aagcatctgc tattactcta aatgcaattc cctttagtat agctattttc
    10981 tcattatttt catctacacc aaaagagact attttaaata ctatatcttt gttatgtgac
    11041 tttctagcta caatgtctcc taccttcata gttaaacctc caataaattt ttatcttagt
    11101 ttatactatg ttcgatataa aactttgttt cctaatgtca aaaaaacatt tcttataata
    11161 cttcttttat cactcttaaa gcatttttat agtatatctt ttctatatca tcttcactaa
    11221 acccttcttt ttttagaggt tcatacagtt tacccatctg agatatatct tcaatctcaa
    11281 cttttgaatc tattccatca aaatctgacc ctagagatac aacatctatt cctccaacat
    11341 ttacaatatg ttttatgtgt ctaaccatat cttcaagttt actttcactt ttatcactta
    11401 aaaataagtg gaagaagttt atacctgtaa ctccaccttt atttgctaat actttaatca
    11461 tatcatcagt taaatttcta gaatgattca tcattgctct tgaattagaa tgtgtagcaa
    11521 ttattggttt agaagaaatc tttgcaattt cataaaatcc accatctgaa atatgagaaa
    11581 catcaaccaa cattcccaat tcattcatct tatgtaccac ttctcttcca aaatcagtaa
    11641 gccctttttc tctatattct actttattat gaggaaagct caattcattc acatgattcc
    11701 aacttattgt catcattcta acccctaagt cgtaaaattt ctttaaattt tctaatttac
    11761 cttcaagtac tgcaccttcc tcaatagata aaagagctgt aagtttacct tctgattggt
    11821 ttttcataat ttcttcatag ttagtagcta aagctatttc atcactattt tttttcattt
    11881 cctcatgaaa tttatttgcc atactcatgc aataatcaaa aggatgtttt acttcttcag
    11941 tatcaacaaa aagagcaaat gtttgagcta atgaatcccc ctttttcaat ctgtctatat
    12001 caacagaata tttattagac ttaagttcac ttgtttctac attttccatt aattttgcta
    12061 tagtatcaca atgtaaatca agaaatttca tatttatacc ccctgtatat tttatattta
    12121 tatctaaaaa tagaaatagt tgtccttata gacaactatt gacaactatt ttttaatatt
    12181 ctttttaata ttacttaatc ttattcttct tttaagttag gtattacttt tttaacttct
    12241 tcaatttgat gccatgtttt atctatatta ttttcactaa tatatttttt taaatcagtt
    12301 aaagatgtaa aatctttaac tgataaattg tcatcatcat ctgcaaatct taaaataaac
    12361 atgagactcc cttactttcc acaacatttc ttatattttt ttccgcttcc acatgggcat
    12421 gaatcatttc ttcctatttt tgcttcttta actattgttt tagacttctt ataagccttt
    12481 attatttctt ttctttggtc tgctggtagt atatcttccc aaccttgtaa attatatagc
    12541 caatgagcct caacagttac catattgtta tataattttt cccaatctat ttttatagaa
    12601 acttctttat cagcttctac ttcttctaaa actatatctt cttttaagct ttcacttatt
    12661 ccatctatga atcccatgaa aaattcatta gtagttccaa atttttcagc caattcactt
    12721 actttacctt caacaacatc cattttatta tttaatattt cattgtatat tccagcttca
    12781 actttcaaat attcttccca gaattttact tctgcttctt gactttcatg attatcactt
    12841 aaatttctcc attcagtata taaactcata ctttttcctc ctaagtatac ctatattttt
    12901 atagttttta tagtataaaa tcttttagtt agattggaag tttcttcttt ttgattatac
    12961 catattctat ttaaagttaa ctcttatatt atatcataat taaacaattc ttttaatact
    13021 tttatagcat actcttggtt tataaaataa gggcttgtcg caattgttat tactttggct
    13081 tttttaatta agttttttat cttgtaaaat ttgagttcaa atggtatata gtccatatca
    13141 cttgaaaata tatccaaatc tatatcaaga acaaactctg attctacttc taaatcaaat
    13201 ccatatagac tatctattat tattaattct gaaaatatgt tatattttaa agcaggtttt
    13261 ataaaacttc ccacatttaa aacttcattt gtatatctaa atacatcttt tagattattt
    13321 acatctacat catagttctc aggttctcta gtatccttat gttggtctac atgcactaac
    13381 ttgcatcctt ttgtaaactc accctttaac aaagattttg cccaaaaata aaatgcatgg
    13441 ttatgattgt caaacacata tatcttttta tttcccagga catattcgac catattctcc
    13501 aatccttttg cttttatttc cttattttca tctacttcat taaatactac ctcattgcct
    13561 aaccttacat catttaatgt accatttatt agttttggaa cgtatatgct cttattttct
    13621 cttttatcat atgaaaatac attatttcct actggttttt ctatataaaa tccactatat
    13681 tcataatttt ccataaaact ttatttcctc cattgttata taaatatata agttataatt
    13741 ttaaaaatta aaatgcactt attgtaaatc atatcatcta ttatagaaaa catcttatat
    13801 aactaaatat aatatcttct ctcaatatgt attatacaat aagtgcaaaa tatatttata
    13861 taaaatatac tacattccta taacaaaacc atcagcttca attggaaggt aactaactct
    13921 tctattcaac tccacctttc caaaaaatac tacccaatat ggtctaaaaa tactttcaat
    13981 tttaactatt tcaatctctg ggattcctcc cataaaccta tgagttaatc tcatagccat
    14041 tttatttgcc tttgctttta ctcttcgttc gtccttatca ctatactgca caatattaca
    14101 atccacattt tttacaactt catatgtagg aaaatcttgt actatactac cagaacctgt
    14161 agaaccattc actaaaattt ttaattcatt tatatcatta gaatatctcc tattcttgaa
    14221 aatggatttc gttttgtgaa ttttaagtgt gataatttta aactctatat aatgaagtct
    14281 aacttcatga gcatatttgt tctttaatat tagcttacta aataaagaat tttttccact
    14341 tttagctatc tctatagctt cttcttttgt aattcttata ggtaacgctc ttatatccat
    14401 aaaatcattc tctttcttat ttttttcttg tcatttttaa aactctatat atataatcaa
    14461 aaaatattac aattacaact gccataactc catatggaat tatttttcca actataatat
    14521 catttctagt caatatacca taaaatataa atcctgttat taaaaagtac aataatacta
    14581 gaaaaccgtc tattccaagc cattttattt ctaaatcttt ttttacctta gacactaatt
    14641 tagacatagc agtttctcct tttatagatt attttgtgta tttaaatttt caccgattct
    14701 attaccaatc atccatgcaa taaatgaaaa aggtatagct atcacaagag gctctaactc
    14761 aaagctctga actggaaata ttttaaaata accaaaagtc actccaccaa caatcattcc
    14821 taaaatacca cccatagatg ttcttcttcc atttccaaga agcccaccta ctataggtac
    14881 aaatgccgaa cccatccaaa tacttcctac aaatatacat gcatcatata cattttcaaa
    14941 tctaaatgct acgctaagcc ccataactgc tgctaaacat acacctattc tagtaaactt
    15001 tactaattgt ttctgagata actctttttt tgtaattctt ccatatatat catttgctaa
    15061 tgtagcccca gctgctagat aatagctatc caatgttgat attatagaac ctaaaattcc
    15121 tattataaat attactctta ttccttgtgg taatgtatct aaaactaaag ttatatatcc
    15181 agcagctgga gccatttctg gataagtagc tcttactata agtcctgtca tagttaacac
    15241 catgtcaaaa catatccata gtgaaaaaca ggttaataag gctcttcttc caaccttagg
    15301 agaatctgct gctgaaaatc tttgataaaa agttgggtct gaataagctc caaaagaata
    15361 tacaattaac atcaatgctt tcatagctga catacctttt gtagggtgca taagctgtgg
    15421 tgcatctttg agtgcttctg taagaccttg aaaccctcct atttgttcaa attgacctgg
    15481 cataaccata gttaaaccaa ataccataca aaaaaacaat accatatctg taatggcaac
    15541 tcccataagc ccaccaagta ctgtaagtac aacgactact cctaccatta ttaatcctat
    15601 taattcttta tctattttcc atgctacgtt acctatagtt cccatagcag ttatttctcc
    15661 aagttgtgca caatatataa acatcaaaat tgccccaatt atagcaacat gcttgccata
    15721 aaaactttcc agtaaatcag gaactgttat attggttctt atatgaatcc ttggaccaac
    15781 ccacaatgct aaaggcattc tcccaatatg tgcagctatt gaccaaactg cccatgcaga
    15841 tattccatat atagccgcat actctacact acctactgta ccaactcctc cataccatga
    15901 tgcaaccaaa gttcctacta ttaaggacca tggtaaactt cgatttccta ggtaataact
    15961 ttccatatct tttacatttc tagaaaaata ataacctatt cctaataata taaatgcaaa
    16021 tataactata atcccatttt cccaaaatgt taacatatct aatctccaca ttcttatatt
    16081 ttaattttta actttaatat ctaaatatga acaactctac ccatttccat aaaaataggt
    16141 agagttatat aataattata agtatttaag taatcttgtt attaagcaat tttattgttt
    16201 aaacctttat tttctctttt atttatttta tttaaagcat aagtagcaaa tatccctata
    16261 actgcacata ctacagataa aataaatata tatgtatatc ctgtttgtcc atatttatct
    16321 aaccaattac ctgcaatagt gtaataaaac gcatctggag aaaatcctat aaatgaagca
    16381 aatccaacta ctgctcctgt agtttctaaa gatatattag actctgaaac agatgcaaag
    16441 tatatacctc taaatccaaa taaaattaca cttagtatta tcatgtttat tacagctatc
    16501 catataaatc ctgtactctt aggaataacc aagtaagcag ctgtacttac tgtcattaat
    16561 ataaatccta caaacattac tcttatagat gaaccaactt tatctgttat aacaccagca
    16621 actggagatg ctcccatttt tataacataa gttcttatta ttgataaagc agaaactaga
    16681 gtcattggca ttacataaac ttctgataag tatggactta aataagttaa acttgagaat
    16741 actaaataag ttgaaaatat tataagacca attaaccaag cctttggtat tgtaactact
    16801 tttaacaatg acttaatact taatccttca tctgatgctc cactatcttt tttctcttcc
    16861 actaagaaaa atagaagtat accacatatt atagatgctg cagaatataa ccatactaca
    16921 tatttgaatc ctactgttat ttcagcaaat ttaccaaaga aataaagacc tataaaagaa
    16981 attaatgtac ctgatacacc tgataaccct tcataaaaac caaatattct tccttgctct
    17041 gactcacttc cttgcattct aacaacctta acagaggctg agaagaaggt taatattgtc
    17101 gtaaatcccc ataatacaaa tattaaaagt atcatactat atgatgggaa tgtagagaaa
    17161 taaaatccaa gtatccctga agcaattaaa gaaaaagtta ttaattttct aactgagaat
    17221 ctatctgcaa accatcctcc tataaaataa gataccattc ctactaatcc atataaactc
    17281 aataagttcc ctatttgtgt atgacttaat gctaaagctt gttgcattgg gtcataaaat
    17341 gatgatttca tatatggtaa actatacata gctgttgttc caaatgccaa tatgaatatt
    17401 atcaaaaatc ttttaacatt atttttgctt tccattgatt ctctctcctt aaaattttat
    17461 tgtttataat ctcattcata taattttatt ttaatttttc tattgcttgt ttaaaatcat
    17521 ttattatatc atttgaagat tctaatccta cagctattct tattagattt tccccattca
    17581 tttgaactaa tgtatgctct tcccctaagc ttgttgctat tgttgcaagc tctaaagagt
    17641 tcattaatct tctactagct tggtctcttg tcaatccact gataccatcc ttaagtttaa
    17701 atgaaactac gcctccacct aaaccattca tttgagtttt acaaagctca tattgaggat
    17761 gtgattttaa tgctggatac tttaccaatt ccacattctc ttcttcctct aaaaactctg
    17821 ctactgctaa tccattttca caatgctttt tcatcctcat atctaacgtt ttcatacccc
    17881 ttaaaatcat ccatgcactc attggtggta aagatgctcc tgtaaaacaa cataaacttg
    17941 gccatctaac taaatctatt aattcttttg aacctgctac tgctccccct aaagtatctc
    18001 catgtccatt tataaactta gtaagactat gtattactaa atctgcacct aaatctattg
    18061 gtctttgtac aattggtgtc ccaaaagtac tatcaactat agctaagctt ttattttcat
    18121 gagcaatttc tgctattgtt tttatatcta ctaagtctaa tgatggattt aaaggacttt
    18181 ctatataaac tattttagta tttggtctca ttgcttcttt tacattttgt ggatttactg
    18241 catctacaaa tgtaacttca actccaaatt tagataacaa ctcagacatg aataatttag
    18301 tgtgtgaaaa tattccatct gaactaacta tatggtcacc actttttacc aatgctaata
    18361 tagtagatgt tattgccccc atgcctgatg tactagatat ggcactttct gcattttcaa
    18421 gcatagccaa cttagcttct aactctgtaa gagtaggatt acactctctt gtatacacat
    18481 atccccatgt catacacaac tcttcaaaat gctctactgt ctttgcagca aatgttgctg
    18541 tttgacatat tggtgtagcc aatgcacctg ttgttgggtc tgcactatgt ccccaatgta
    18601 ttatttttgt ctctaaatct tgattaatat tgttactcat tttgatttcc tcccattttg
    18661 acttatttat acaacgtttt ccctgccaaa tttcatcaat ttagcttagt cttaatacat
    18721 tttttcaagt atactttttt atttgttata atttctaaaa atatgacact tattttgttt
    18781 ttttacagtt ctaattataa actctatagt aactatagag tcaatatgtt tttgatattt
    18841 tttttacaat gtttaaaata gtcaatacaa aaataggtat atcttagaat aactaaagtc
    18901 atcctaagat atacctattt ttatatcaca atataaataa ttacatataa gcattagttt
    18961 ttttatcata aatagatttt tcaagattta tcattttttc ataaatatat tatagttaaa
    19021 tattttaata ttacctaaca tctacaataa aatatactct taaaataaaa ttatataatt
    19081 aaaaatactc tataaaagct aaaaagtttt tctattcgct acatctaaaa ataattagat
    19141 agaggtataa atgtatttag tttatttata cctctagtct tttataaatt atatctctac
    19201 taaattgatt ttttagcacg aataagtgtg ctcttggaga taccagtcat cttcacaacc
    19261 tctctatatg gcttagaatt taataacccc aatgcatatt gtatctgttg attgctgtat
    19321 ttctttggcc taccatcttt aaagtccttt ttctttttag caaattcttt tcctaattga
    19381 gtacgttcta ttatcatatt tctctcaagt tctgcaactg caagtaatgt agtcaaaaag
    19441 aatttaccca ttgaagtatt ttctaaaagg cctatattta aaatatgaac acttacttct
    19501 ttagcaaata aaccttctat tagttgtatt ccttctgatg tacttctagc tagtctatcc
    19561 agcttagttg caacaagtaa gtctcccttt tttagagatg atataatttt ttcaaattta
    19621 ggcctgtttg tagtagtgcc tgtataagtt tcctttatta ttttagcatc attatattta
    19681 ttcaaaattt cttgctgttg ctgatacaaa ctgttgttgt ctgtttgatt ttttgtactt
    19741 acacggcaat atccatatat aataatcatc acttcctaac ataaagattt tgtacagaag
    19801 ttacccagaa gcttgttttt acttatcttt atccatttta ctaaaacgat ttttgggtgt
    19861 caatactgtt aacttttgac accataataa ttcctttata atttgtttga ctatgtcaaa
    19921 aattaccaat ataatgaaat taatgagttt atacaaattg gaggaaataa tatgaatagt
    19981 ctgttaaaag aagcaataga tgaattaaaa aacatccatt gcggggaagt ttttttatta
    20041 aaagaattgt tcaaaggcta tgaatggaac aggttggaga tgaatatcag attgaaatta
    20101 ggaaaacttt ttttgtatga agtggtaact aatcccaatt taaatgtaga ggtcatcaat
    20161 aaaaattctg cttatcagca aatgtataaa aaaaaatagt gacttaaatg tatataatag
    20221 tataatcttc ttattgattc aaaagttaaa acttttaata caaaacaatc attctatttt
    20281 aacatttatc aatatgtcta aatttatata attaacacat acgaatagta ttgtttaaat
    20341 cacattattt aagatagata aatcttaacc ctactccagc ataaatacta aaatagggct
    20401 tagaattatc tatattttta tcccataaaa tgcttagctc ttcctcttga ttgaacatat
    20461 atatctgggg aaatacaata gaagcgtaaa tttataaaat ttccaaagtc agaaaacttc
    20521 agttcaaact taggtatacc aaaactttca ggtgctattt cgtcaacgcc aaaatagatg
    20581 ataattccat tatctgtaag ataaaatgct tggtcttcag gtataactaa ctcaacatta
    20641 gaataataca tttctctatt ttcatttatt tgagatttaa tgtaatcagt aattatatta
    20701 atataatcaa ctccatctct aaaaatgtca ttaagtaaaa gttcttttcc agttgtcaaa
    20761 tctatattgt aattattcaa tgtattgtat ttaggcccat agttattagc aaaccccata
    20821 aggtttaata tcatactcag tacataattt ttattaaaag taactgcgta actggtagat
    20881 acccaatagc tttgttttgg aagtgaattt tgagaagctg cgttattata ttctcgttct
    20941 tcttcctgta agccattttt aaaattattt acagtcattc ttacagcatt gtttatgtta
    21001 tttaaaataa ctgggtttac atacgtggat tgatttctaa tattgtatat aacaggatac
    21061 tttacatcat atgaaaaata cttacttctt tcaaaatctt cattaaattg tacatataat
    21121 atattatatg tacaggtatt tgtagattga taattttcca ttaaaatcct ccccctttta
    21181 taaaaaaatc agatacaata taatatgctt aaaacagtaa taatgttaat ggtaaatgta
    21241 aagaatagaa agtatctaaa acagcaaaaa atgactaatt ttgtggtata atacatagga
    21301 ttgagagtta ttttatagga ggaaaaatat gttattatta gcagataaat ggaaagatta
    21361 tgaacttata gatatgggaa atggagaaaa attagaacgt tggggaagtt atgtacttag
    21421 aagaccagac ccacaggttg tatggccaat ggaatctgaa tgggctttat ggaaaaaccc
    21481 tcatggacat tatcacagaa gcaataaagg tggaggtcaa tgggaacaca aaaaaagata
    21541 tccagaacaa tggactataa attataaaaa cttaaagttt cacattaaac caactggctt
    21601 taaacatact ggattattcc cagagcaagc agctaactgg gattggatga tagagaaaat
    21661 aaagaaagct aacagaccta taaaagtgtt gaatcttttt gcatatacag gtggagctac
    21721 tgtagcatgt gcatctgctg gtgcagaagt atgtcatgtt gatgctgcaa aaggtatggt
    21781 taattgggca aaagaaaata tagaattatc aggtcttaaa aatcaaacag taagatttat
    21841 agtggatgat gttgtaaagt ttgttgaaag agaaataaga agaggtaaaa aatatgatgc
    21901 aataataatg gaccctcctt cttatggaag aggtcctaaa ggagaagtat ggcaaataga
    21961 agaaaagtta tacagttttg tagatttatg tatgggagtt ttatcagata accctttatt
    22021 tttcttaatt aattcttata caacaggatt ttctccaata gttcttgaaa atgtattgtc
    22081 tacaacaata ggtaaaaaga caaatacagg agatatatac ggtggggaat taggaatacc
    22141 agcttccaga gatggtaaga tacttccttg tggtatattt ggaagatggg agaaaaaata
    22201 atgataaaag tcatatatga agataaccat ttattagttg tagaaaaacc agttaacata
    22261 ttgtctcaag gagataatac taatgataaa gatatggtaa atcttttaaa agactatatt
    22321 aaaaaaaagt ataacaaacc tggaaatgta tatattggtt taatacatag attggataga
    22381 ccagtaggtg gagttatggt gtttgcaaaa acatcaaaag cagcatctag attgtctgag
    22441 caggtaagaa gtaagacttt caaaaaaact tatctagctg ttgttcatgg caagataaaa
    22501 tctaactcag atatactaaa agatttttta tataaaaata aaaaaactaa tatggtaact
    22561 gtagtgaaaa aaagccataa agatgccaaa gaagctgaac ttaactataa tgtattagat
    22621 tttaaagaaa atctaagttt agttcaagtt aatttaaaga ctggtcgttc tcatcaaata
    22681 agagttcaat ttgcaagcag aaaacatcca ttatacggag accaaagata tggcaaagaa
    22741 ataaataaga ttggacaaca aatatctctt tggtccaata agatagaaat ttgccatcct
    22801 actacaaaag aaaaaatgga atttacatgt catccacctg acaaatatcc atggaatata
    22861 ttttaaatct caaatgaggt gattatatga gtaaaaaatt ttcaatagga gaaatgtcta
    22921 aacttcataa aataagcata cagacactaa ggtactatga ccaaatagat ttatttaaac
    22981 ctgttcatgt tgaccaagaa agcaattaca gatattactc aattgaccaa ttttcacaat
    23041 tagatattat aaaatattta aaatatttag gtatgccttt aaaagaaatt aagtcaaagc
    23101 ttgatgaaaa taatgaagaa attctaaaat tactaacaaa taaaattaat atagtggatg
    23161 aaaaaattaa agaattagag cttataaaga aggtactaag cacaaagaaa gaaactatgg
    23221 aaaaaggtat gaaagtagat gaagttggta aaatagtgag aaaacatatt ccacgaagaa
    23281 gtatcttatc tatagagtat gaagaaggat taagtagtga agaaactatg gaactttcca
    23341 gaagacaaat agcaaatata ttagaagata atatatctgt gttctatggt ggagtaagtg
    23401 gacttgtatc tatagataaa tttttaaaag aagataaagt tatttatgac aatagttttg
    23461 ttactgttga aagagactta tttgatataa aatcacaaaa acatataaca gaaatttcat
    23521 ctggtgattt tatttgtatg acatataaag ggccttacaa agaaaattat accgctttta
    23581 agaaactggt tgattatgtt gaatataaaa gaatacctat tgaaaattat atatatgaaa
    23641 tacctataat agaccctctg tctacaaaca acgaagagga acttctaaca gaaattcaaa
    23701 tacaagttaa aaagagagaa attgacttaa gatgtctagt gtagtaatct agtttactta
    23761 cacatcttaa tatagttgtc tattatcaac atatgatttt tctagatagt gattcctaac
    23821 agtgagattt tcaaaaaata ggaggaaata tggctaatgc taattccctc ctatttttaa
    23881 ttaagccata tcattttaat taagccatgt catcacgaag tgcatcaata atattttctt
    23941 tttttatctt attaatagta tacaacattg taataaatac tataaataaa acactaaata
    24001 tactaattcc aatactaaac catggaatca caaaatcaat atcatctgta accattactt
    24061 tatgaattag ccatgaagaa attactgcta taggaagtcc aaagaacaat gctctcatgc
    24121 cataaaaggc acactcaaaa ttcatcatct tttggaaatc atgctcagac attcctacag
    24181 agcgaagcat ggcaagttct ctacgacgta acttaatatt agttgaaatt gtattaaaca
    24241 cattggcaat cgaaatcaga gaaatcataa ttatgaaagt ataagcaaaa acattggcaa
    24301 taaaaatgta attacggttc tcatccatca ttccatacat attgtacaga ttgtactcat
    24361 tcgtaactcc ctcactctca ataatttgct ccatttctgc aactgactga gtaggatttt
    24421 tcgattgaaa gcttaaactt tcaattcctg tagaaacacc aggaatctca aacttatctt
    24481 tggctgaata aggagccaat actctgaagc ttgctttctt cacatctgaa cttctgattc
    24541 ctggaggaga atcaggtggc actactttga caaatgaaat attcacattt tgtctctcag
    24601 gtatctccaa cttgccatta gtttcaggag tgaggctaac attcatagaa gaactgttga
    24661 aaatatcaat aatattatta tcctttgtta ccatagaaac agcaatcagt tttgcatttt
    24721 gtccagtata ttcttctgct ggtaatccaa gacttttaat aatattcaga taagttctat
    24781 catcaagaaa ttgcatatct actggtaatt caagcatttc actcatagaa tgtgaaccct
    24841 catagttcaa ataatcgtct gtaagctcac ttactttggc aacacatgaa taggtcataa
    24901 gtttctgata caaaccttcg taaacaccat ttgcagtttt tagcttatcg taaagcttca
    24961 acatttcgtt gtctttcata ccttgtgtag aaaaaataat atcctggtca gtaacctctg
    25021 acctctgatt tgacatctgt tttaggtctg ctactaaagc acttgtagat ataaatagaa
    25081 ctatgcttaa aacaagcgac aatacaatac ttcgataacg tttcttgttt cttttaaagt
    25141 ttttcatagc aagaactccc tcaagaccat aaactcgctg tgttagtttt gatgtcttca
    25201 cagatttaga ttcaattttt acctcatttg tctgacgaat acactccatc actggtgtgt
    25261 tggcagcctt tcttgctggt atgtaagcag aaattaagat tgtaaccata cttaccactg
    25321 ctgcaacgat gattgcaggg atagacaatg ttaatgtcaa cggaacgttt acatagagaa
    25381 tatttgcaaa gtttttagat atgacagaaa tcacaagtcc aatactgact ataccgattg
    25441 caacaccaat tggtatacca atcgcaccaa tgcaaattcc ttcaaatagc accgaatttc
    25501 gtaactgttt tgctgtagct ccaacagatg aaagtattcc aaattgatgt gttcgctcgt
    25561 tcaatgacat gttaaatgaa ttatatatta gaaaaattga gcctagcatg attatggcaa
    25621 gcacaatgcc accaaccgag tacaaaaaca tattgaacaa attgttatca gagacaccta
    25681 tgaatcgcaa cacattgtca ttgaaaatgg aagcatggtc tttagtgcta cttgtgtaag
    25741 agcgaatctg atgtggattt ttcaatgtga caaaaaggct aagactgtca gtcttgactt
    25801 gtttatcttc tttggttatc aaggtatatc ctggtgcaga agattcttca aatacgggct
    25861 ttctacagat acccacaact gtataagttt tcttatcctg tggcacaaaa acttcactcc
    25921 cagcagtgta tgggtcactc tgaccaagct tttcattttc actcattcgt ttccctacat
    25981 ccaaagaaat agtatcccct actgcaaatt tgacaccacc atctgtcgca actttacttg
    26041 aaacaagaat ctctctacta ttttcaggta atcttccaga aattaaagtt ataggcaaag
    26101 tatcaaaagc ttctttgcta aatccagcaa taaaaagata tggtttatct ggatttctgt
    26161 taccttcaag tgttgcatag ccaatatttt caaatgttgc agtatttgca acttcatcat
    26221 tgactctttg ttcctgtaca aaagaagaat ccacatccaa aaactggaca tgccaatcac
    26281 catattttat aatagctcca tttatcatat agttcatcaa agaaactcca aaggtagtaa
    26341 ctgctgtaat catagcagct gacagaacaa ctcctataat tgttacaaga gttcgagttc
    26401 gattcttttt caatccttgc agagcaactt tgttaaaaat gttcattaac gattcacctt
    26461 cctctcatca cgcactactt ttccatctga aatacagata attctatctg cctgtaaagc
    26521 aatgttttca tcatgtgtga caataatgag agtttggtga tatttactat ggctctcttt
    26581 cagtaactgg ataatctctt gtccattttt actgtctaag ctacctgtag gctcatcagc
    26641 aagcataact gctggtgcat tcatcaatgc acgaccaatg gcaacacgtt gctgttggcc
    26701 accagaaagt tgatttggta aatgtgtttt tcgttctttt aaaccaagta attctaacaa
    26761 gtcatttaac cgttcttgat tgaccttacg cttatccata agtataggca atgtgatatt
    26821 ttccactaca tttaatgtgg gtatcagatt gtgaaactga taaatcaatc caacttgtcg
    26881 cctacgaaaa atagcaagtt tttcattgct ttggccatat acatcttgcc cctccagata
    26941 tatctttcca cttgttggta cgtctactcc tgcaatggaa tgtagtagtg tagatttacc
    27001 agaaccagaa gaaccaatga ttgcagtaaa ctcccctttt tcaatagtaa gtgagacatg
    27061 gtcaagtgca gtaaccttat tttcattctt accataaacc ttacataaat cttcaatctt
    27121 taaaaacgcc attatataag tctcctttca tattttataa atatatcata gaactttcaa
    27181 cttacattta agtgacattt aaatgagaga ttcgtcactt tggaaaacga ataacaaata
    27241 ttgcaccacc ttgtgggtga ttttgtgcag taattgttcc tccttgtctc ataataatcg
    27301 tcttacaaag tgccaatcct attccataac cggctgtgct tgaatttttc ccacgataaa
    27361 atctgttaaa tagacatggt aaatcttctt ttttaaagcc tgctccgttg tcatgaatag
    27421 taagttgagt aaacaaaaag ttgtcctcac aaatgatatc aatttttcca ttatcaccca
    27481 cactctccat gcagttttta aatatatttt gaattgcttc tgaaagccaa tttaaatcac
    27541 ccaaaatcct tactcctttt ggtatatcaa tatgtaaagt gatattgtgc agttccatgg
    27601 gaatcaaaag ttgatgaagt gcagaactaa tcaaattatt cacatcaatt tgttcttttt
    27661 gaaagactac aatacctgca tctaaacgtg ataattttaa aagggaagta atcagccagt
    27721 ccattcgtat aagtaattcc tctgtttctc gtatcaactc ttttctctca tcttcatcag
    27781 aagtattctc taacagtgac aaaataagag ttactgatgt aagaggagtg cgaagctggt
    27841 gggctatgtc agctagtgag tcagcaaggt gttctttttc ttttttcagt gcgtaatttt
    27901 gctctctaat gcgtaacgtc attttagtta tttcactttg tagaatagaa agctcaccct
    27961 catctgattc agcaatatac agatggttag cattatgaag cacaagatta atttgttctg
    28021 aaatctgtgc aatacttttg tatcgatttt tagtaaacac aaaaaatgct attccaaagg
    28081 tagtggcaga aacaatagaa agtattccag tcataatact gattgcaaat cccagggcta
    28141 cagtgagaat agtaattaat aagaataaaa tagcaaattt tcgaaactct ctatttcgaa
    28201 acataacttc cccccaatct ataccctgtc ccacgaacag tcaaaattat ttgtggactt
    28261 gaagggtcat cttctatctt ctctcgcaag cgtttgatgt acacagtcaa tgtgttgtca
    28321 ttaacaaact cacctgccgc atcccacaat tcatcaagta atcttaccct cgtaataata
    28381 cttttaggat tgctaataaa caccaataac aaacggtatt ctaaagctga aagaaaaacc
    28441 tcattgccat tctttttcac aacaccagtt gctgtatcta cataaagccc acaaatttca
    28501 aaagttgacc caaaacgccc actttttcgt agtgctgttc taattcgcgc aatcagttca
    28561 cgtggacgaa aaggtttagt aatataatca tctgcaccca tattcagtcc agtaacaaca
    28621 cttgattcat caccggaagc tgtcaaaaag ataacaggaa tgtcttgctt ttctttgatt
    28681 tctgtgcaaa tcgtaaaccc atttccatca ggtaaagaaa tatcaataag tgccaaatca
    28741 aatttattct tagcaatctc tacaagtgct tcacttcttg tggatacatg ggttactata
    28801 aatccttctg aattaagcaa aagaataaga tttttggcaa ttgctttatc atcctcaact
    28861 aaaaatatcc gtttcattta ctttcatcct cctttatttt attagtttta tatattccag
    28921 cagagcaata gtacactact attttactta tcattacaca attttcagta gtttcttttg
    28981 ttcttatata atccatgcat aatacctcca ttgatttatt atatttctta taacataaat
    29041 acacaagata agacaaaaaa cataatgtaa gtgcaagttt tttaactcac taacttataa
    29101 acatacactc ctactaacac tactttcttg atgacacatc ttacaagctt atattcatac
    29161 atcaatgaaa aattaaattt atccatttat taaaataaaa ggcaatagct tctatagcta
    29221 atgcccttta tcttaaaatg atttaaatac tatacaattt ttataaatgg ctataaataa
    29281 actcgtatag aatataaaat ttatctacag tcttttgtta tacaaaattt ataacctctt
    29341 atagaacaaa tttaccactt tgcaaactta tcacttttac tcagctatat tataaacttt
    29401 taatttatct ttgtttaaat gtttcttctt aacttctact aaagctttta gcgtatccaa
    29461 agatgtcatt atttcagtag aaaattcaat ggctgttctt ctcatcttaa atccatctct
    29521 tgtagagtca tttcctttag ttggtgtatt tattactata tcaacttgtt tatttcttat
    29581 agcatctaat atgtttggtc taggttcttc aattctattt actatatcag cctctattcc
    29641 attttctctt aaactcttcg ctgttccttc tgtagcaaca aaagtatatc ctaattcttt
    29701 catatctttt gctatctcta taaactcatc tttatcatga ttatttacag tagcaagtac
    29761 tactcctctt tcatcagaca tatgtctacc tgctgccaag aaccctttat ataaggcttc
    29821 ttctaaattt tctcctacac ctaggacttc tcctgtagac ttcatttcag gtcctagact
    29881 tacttcaact ttagataatt tagacattga gaatactggt acttttactg atactaattt
    29941 tggctctttg taaactcctg taccatatcc taaatccttt aatttagctc ctaacataca
    30001 cttagtagcc aagtcaacta ttggtactcc acttacttta cttatatatg gaactgttct
    30061 actagctcta gggttaacct caattatata taattcattt tgaaattcta taaactgtat
    30121 attaaccata ccaagcacat taaggtctaa agctattttt ttagtgtatt ctactatctt
    30181 ttcttttatt tcatcagata tattttgact tggatacatt gttgtactat ctccagagtg
    30241 aactccagct ctttctaaat gctccattat accaggtatc aatatatctt ctttatcaca
    30301 tatagcatct acttctattt ctctaccagt tagatattta tctataagta ctggattttt
    30361 atggtctcta tcaaatgcat catccaaata ctgaattagt ttttcttcgt tataagttat
    30421 ttccatacct tgaccaccaa gtacataaga tggtctaact aatactggat aacctaactc
    30481 cttagcaatt tcaacacctt cattagttgt ccatacacct tttccttttg gtctatttat
    30541 atctagtctc tctagtaaat catcaaattt ttctctatct tctgctgcat ctatatctct
    30601 aaagtctgtt cccagtatag gtatattttt ttcatgtaaa aacttagcca attttatagc
    30661 tgtttgtcca ccaaactgta gtattacacc ttctggtttt tctttttcta ttatatttaa
    30721 aacttcttct tctgttaatg gttcaaaata caacttatca gatgtatcaa aatccgtact
    30781 tacagtttca gggttattgt ttacaattat agtttctatt cccatttttc ttaaagattt
    30841 tacacagtga actgaacaat agtcaaattc tattccttga cctattctta ttggaccaga
    30901 acccaatact acaacttttc tcttatcact aactactacc tcatcatatt gttcataagt
    30961 tgaatagtaa taaggtgata gagcttcaaa ttctcctcca catgtatcaa ccattttata
    31021 aactggttgt atattatata aacttctcaa ttcatataat ttttctgggc ttatcttcat
    31081 taagtcagat atacctttat cagaaaatcc ctttttcttt aattcatgta aatattcttt
    31141 atctaagtct tctattttta gaacttttaa cttttcttct tgttctacta tccatttaaa
    31201 tttatttata aaccacttat caacaccagt tatttctgat atcatttcta ctttatagcc
    31261 tcttcttatc atttcagcta agtcaaatag tctctcatca tcaggtacta ctactctagc
    31321 ctttaattct tctaatgttc taccttctga tggagcatga accaatgaat attttcctgt
    31381 ttcaagtgat cttatacctt taagaatagc agcctcaaag ttacttccta tactcatgat
    31441 ttcaccagtt gccatcatct tagttcctaa ttttctattg gcatgtctaa atttatcaaa
    31501 tggccacttt ggtattttta ctaccacata atctaatgtt ggctcaaaac aagcataagt
    31561 ttttttagtt actgcatttt ctatctcatc caaagtataa cctaaagcta tcttagctgc
    31621 tacttttgct attgggtatc ctgtagcttt tgatgctagg gctgatgacc tactaactct
    31681 tgggtttatt tctattatcg catattctaa actatgtggg tttaaagcta tttgaacgtt
    31741 acatccacct tgaacctcta ttgcatttat tatgtctatt gatgcctttc taagtagttg
    31801 atactcttca tcacttagag tttgacttgg agcaacaact atactgtctc ccgtatgaac
    31861 tccaactggg tctacatttt ccatattaca tacagttata cagtttccat ttccatctct
    31921 cattacttca tattctattt ctttccaacc ttttatactc ttttcaagaa gaacttgtcc
    31981 aacaggactt agctgtagtc catgtgaaag tatttctctt aattcttcct cagtatcagc
    32041 tatacctcca ccagttccac ctagcgtata tgctggtctt acaactactg gatatcctat
    32101 cttattggca aactcaagac ctgcttctaa atttgtaact atatcactaa ctattactgg
    32161 ttgatttatt tctttcatta cttctctaaa taaatctcta tcttcgccct tttttataga
    32221 ttctatagat gttcctataa cttttacatt atatttatct aatataccag catcatgtaa
    32281 ttctacagct aagtttaatc ctgtctgacc acccatacct gcaagtaaac tgtctggtct
    32341 ttctttttct attatttttt ctataaattc tattgttaat ggttctatat atattttatc
    32401 tgcaatttct ttatcagtca ttatagtagc tgggttacta tttactaata caacttcaat
    32461 tccctcttct tttaatgctt ggcaggcctg tgttcctgaa tagtcaaact ctgcagcttg
    32521 acctattatt attggccctg accctaatac taaagttttc tttatactat ctaatttagg
    32581 cataactctt tctccttatc taaacaattt tcacagttct tatcttcatc atctatacaa
    32641 tcaactttaa ttactttatt ttatcttaag ttcaaattta ctcatcaata catctattta
    32701 atacatctat gttaattaaa ccaaactttt tgatttaagt tattcttgtt ttttagttag
    32761 ctattctaat gttgtttatt ttactaattc taaaaattta tcaaaaatat agtcattatc
    32821 ttttggtcct gggcaagctt caggatggta ttgaacactg tatacatcta gcttctcatg
    32881 tcgcattcct tcaacagtat tatcatttaa atttatatgt gtcactttca tttgttcagg
    32941 aacttctgaa acataataac catgattttg agaagttata aatacttttc cttcttctaa
    33001 atctttaact ggatgatttc cacctctatg cccaaattta agtttagcag tttttcctcc
    33061 aagagctaaa gctaaaagtt gatgtcctaa gcatattcca actataggtt ttttaccaat
    33121 aagagctttt atattttcta taacatcttc taagtcttct gggtctccag gtccatttga
    33181 caagaatatt aagtcagggt ttatactcaa tacatcttct ggagatgttg tagctggaaa
    33241 tattgttata tcacatccac gagctataaa tgatcttaat atatttcttt ttactccaaa
    33301 atccataaca gccacttttg gaccatttcc ttttatatgc tctacttctt ttcttgtaac
    33361 agttctaact gcttctgtat ttgaaaactt atccaattta tgtttaacat cttctaattt
    33421 actgtcttct aatgtaatta tacctttcat tgttccatta tttctaagta tcttagtcaa
    33481 agctcttgta tctattcctt caagtccgat aactttattt tgctttaaat attggtctat
    33541 atcaatttca catctaaaat tacttggaga atcacttttc tcccttacta tgaatccctt
    33601 tacttgaact ttttcagact ctgctgatga taaatttata ccataatttc ctacaagagg
    33661 ataagtcata gtaactattt gaccatagta agaagggtct gttaaaactt caccatatcc
    33721 tatcatcgaa gtattaaata ctacttctcc tacactttct tctaaatatc caaatgcctt
    33781 tcctataaaa actgttccat cttctaatat taaccttgct ttcatactac agtacctcct
    33841 ataattctct ttagacaaac taaagaacat taactaaata taagctttta aatatctata
    33901 tctatttttc acttttacat tctagtttta tacttattta catttatttt ttatcttttt
    33961 ctattatact acaaatttct taaaaaaaag aagttatctc aaaagtaaaa actcctagaa
    34021 aaaataacct ctttttttta tttaataatt aaattcttaa actatacaat caacttaatt
    34081 tctattcagc tatattgtaa acttttaact catctttatt taaatgtttc ttcttaactt
    34141 ctactaaagc ttttagtgta tccaaagatg tcattatttc agtagaaaat tcaatagctg
    34201 ttcttcttat cttaaatcca tctcttgtag agtcatttcc tttagttggt gtatttatta
    34261 ctatatcaac ttgtttattt cttatagcat ctaatatgtt tggtctagat tcttctactc
    34321 tatttactat atcagcctct attccatttt ctcttaagct attagctgtt ccttctgtag
    34381 caacaaaagt atatcctaat tctttcatat cttttgctat ctctataaac tcatctttat
    34441 catgattatt tacagtagca agtactactc ctctttcatc agacatatgt ctacctgctg
    34501 ccaagaaccc tttatataag gcttcttcta aattttctcc tacacctaga acttctcctg
    34561 tagacttcat ttcaggtcct agacttactt caactttaga taatttagac attgagaata
    34621 ctggtacttt tactgatact aattttggct ctttgtaaac tcctgtacca tatcctaaat
    34681 cctttaattt agcccctaac atacacttag tagccaagtc aactattggt actccactta
    34741 ctttacttat atacggaact gttctactag ctctagggtt aacctcaatt atatataatt
    34801 cattttgaaa ttctataaac tgtatattaa ccataccaag cacattaagg tctaaagcta
    34861 tttttttagt gtattctact atcttttctt ttatttcatc agatatattt tgacttggat
    34921 acattgttgt actatctcca gagtgaactc cagctctttc taaatgttcc attataccag
    34981 gtatcaatat atcttcctta tcacatatag catctacttc tatttctcta ccagttagat
    35041 atttatctat aagtactgga tttttatggt ctctatcaaa tgcatcttgt aggtattgag
    35101 ataatttttc ttcgttataa gttatttcca taccttgacc accaagtaca taagatggtc
    35161 taactaatac tggataacct aactcattag caacttcaat accttcgtta agtgaccata
    35221 caccctttcc ttttggtcta tttatatcta gtctctctag taaatcatca aatttttctc
    35281 tatcttctgc tgcatctata tcatcaaaat cagttcccaa tataggtata tttttttcat
    35341 gtaaaaattt agccaatttt atagctgttt gtccaccaaa ctgtagtatt acaccttctg
    35401 gcttttcttt ttctattata cttaaaactt cttcttctgt taatggttca aaatacagct
    35461 tatctgatgt atcaaagtct gtacttacag tttcagggtt attgtttatg attatagttt
    35521 ctatgtccat ttttcttaaa gattttacac agtgaactga acaatagtca aattctatac
    35581 cttgacctat tcttattgga ccagaaccta atactatcac ctttttctta tcactaacaa
    35641 ctacctcatc atattgctca taagttgaat aatagtaagg agatatagca tctatctcac
    35701 ctgcacaagt atcaaccatt ttatatgctg gttttatatt gtatagactt ctcaattcat
    35761 ataatttttc tggacttatc ttcattaggt cagatatacc tttatcagag aatccctttt
    35821 tctttaattc aagaagatag tctttagtta aatcttcaat tttcataact tttaattttt
    35881 cttcttgctc tactatccat ttaaatttat ttataaacca tttatctata cctgtaattt
    35941 gttctatcat ttctacttta tagcctcttc ttatcatttc agctaaatca aatagtctct
    36001 catcatcagg tactacaact cttttcttta attcctcaat gcttctctcc tcagatggtg
    36061 tgtgaactaa tgaatatttt cctgtttcaa gtgatcttat ccctttaaga atagcagcct
    36121 caaagttact tcctatactc ataatttcac cagttgccat catcttagtt cctaattttc
    36181 tatttgcttt tttaaattta tcaaaaggcc attttggtat ttttgccacc acataatcta
    36241 tagttggttc aaaacaagca taagtttttt tagttactgc attttctatc tcatctaaag
    36301 tataacctaa agctattttt gacgaaactt tagctattgg atatcctgta gcttttgatg
    36361 ctaaagctga tgacctacta actcttggat ttatctctat aactgcatat tcaaaactat
    36421 gtggattaag tgcaaattga acgttacaac caccttgaac ttctactgca tttaatatat
    36481 ctaaagatgc ttttttaagc attgcacatt ctttgttact taaagtttga gttggagcaa
    36541 caactatact gtctcctgta tgaactccga ctgggtctat attttccatg ttacatacaa
    36601 ctatacaatt tccctttgaa tctcttatta cctcatattc tatttctttc caacctttta
    36661 tactcttttc aattaatact tgtccaacag gacttaattg tagaccatgt gaaagtattt
    36721 ctcttaattc ttcctcatta tcagctatac ccccaccagt tccacctagt gtgtatgctg
    36781 gtcttacaac tactggataa ccaattgtaa gtgcataatc aagacctgct tgtaaatctg
    36841 taactatatc actaactatt acaggctgat ttatttcttt cattacttct ctaaatatat
    36901 ctctatcttc accttttttg atagattcta ctgaagtacc tattatcttt actccatatt
    36961 tatctagtat gcctttttca taaagttcaa ctgctaggtt tagccctgtt tgtccaccca
    37021 tacctgcaag taaactgtct ggtctttctt tttctattat tttttctata aattctattg
    37081 ttaatggttc tatatatatt ttatctgcca cttctttatc agtcattata gtggctggat
    37141 tactatttat taatacaact tcaattcctt cctctttcaa agactggcaa gcttgtgttc
    37201 ctgaatagtc aaactctgca gcttgaccta ttattattgg tcctgaccct aatactaaag
    37261 ttttctttat actatctaat ttaggcataa atttttctcc ttatctaaat ggtttttact
    37321 atctgtaaat aatactaaat ctatattaat gctaagaatt tattaaatat acaatctaaa
    37381 tcttcttttg ttgtgtattc tggaatgtat tgaacactat atattggtaa ttccttatgt
    37441 ctcattcctt ctatagtatt gtcatttaaa tttatgtgag ttacttctgt attttctggc
    37501 atttctgaaa cattatatcc atgattttga gaagttataa atacctttcc agtttctaaa
    37561 catttaactg ggtgattacc tcctctatgt ccaaatacaa gctttgaagt ctttcctcct
    37621 aatgccaatg ctaaaatttg atgtcctaaa cctattcctg tcaatggctt tttgcctatt
    37681 aattttttta tattttcaac tatatcattt atatcctctg gatttcctgg gccattagat
    37741 aataagatta agtcaggatt tatatttaaa atttcttcta ctgtagtcat tgctggtact
    37801 atagttatat cacagttaca agccttaaga gactctagta cactattttt aacaccaaag
    37861 tctataacta caactttagc tccagtacct tttatatttt caatttcttt tcttgtaaca
    37921 ttttttactg aatctttgtt acaatgctct tctaatttag actgaacatc ttgtaatgat
    37981 acattttcta aagttataat tcctctctta gttccattgt ttctaataat tttagttaat
    38041 gctcttgtat ctattccttc aagtcctatt accttatttt gctttagata tgtgtctata
    38101 tccatttcac atctaaaatt atttgggtta tcactttttt ctcttacaat aagtcctttt
    38161 acttgcacct tatttgattc aacatcttct aaatttattc catagtttcc tataagtgga
    38221 taagtcatag taactatttg tccataataa gttgcatctg tcaatagttc tccataccca
    38281 gccattgaag tattaaatac aacttctcca atagcttcct ttagatatcc aaatgctttt
    38341 ccttcaaata ctgttccatc ttctaaaatt aatttcccct tcatgtaagc tacctcccaa
    38401 atattttatc taagcatttt ctatttctct tacaatatta atagctgatt ctcttggatt
    38461 ttctgctttt gttataggtc ttccaacaac taaataatga gcaccttttt ttattgcatc
    38521 tgatggagtt acaactcttt tttggtctcc aacctctgct gatttaggtc ttattcctgg
    38581 gcaaactgtt ataaaatcag ttccacatgc ttctactatt ttatctactt cttgcgctga
    38641 acaaacaacc ccatctattc ctgattcttt tgccagttta gctctcttta tagctaactc
    38701 ttctgttgtc atattacatc ctatgtcctg taaatcttgg ttgcttaatg aagttaaaac
    38761 tgttactcca actactaatg gcttttctat atttaatctt tctgcttctt ccctagcaat
    38821 tagtgcacat tgtctcattc cttccatatc tgatacatgt attgtcatta accaaacatt
    38881 atctctcact gcagctttca ctgcactttt catagtattt ggtatatcat ggaatttaag
    38941 gtctaaaaat atttttttcc ctttttcttt aagataatct attgttttac cttttgtggc
    39001 aacgtattgc tctagcccta ccttaaatat gtctacacta tcttctaatc tgtctattag
    39061 ttctttagct ttatcaaatt cgtcagtatc aattgcaact atcaattttt ctttaccatt
    39121 tatcattttt acatctcctt tttaataaag cctaaataat cataaaaaaa tcctcacacc
    39181 aaaagaggca taaggatttt atctatacat aactctattc attagctatg agatatcttt
    39241 cccagtctct ctggactagt ttaaaagact ttattatatt taaattttta ttttatactc
    39301 atcaaaaacc tctagaagat ttactagagg ttatatacta acattatcta taacccttta
    39361 gtctctctgg actactttaa ggaatttagc tgtaatattt ttctttattt atataacttt
    39421 atgtatctat aggataattt aaagttttat cttttttata attaaaccac taattgcttt
    39481 ttatgtcaat aagtctttga attattcttc atctttatca aacaattttt tctcaaaata
    39541 tgattctgca ttatatattg cagcaactgg attatgccca gttactctat ctttgacaac
    39601 taaagttgtt ataggagctt ccatatattt catggctaaa gtatcgtgac caacacataa
    39661 tccaagaatt acattaaggt caaccttagc ttcatttaaa aataaagctt gtcctattgg
    39721 attgcacatt acctcatctg aacactttga tattgtttga gatgcttcta gtccaattgt
    39781 actcttaggc attgccccat ttttacatat aacagaaaat acttcaaacc catgatgttc
    39841 aaatattttt tgcactgttt gcatttctct ttttagtcca atacaaaatg ctatgccaat
    39901 ctttttataa ccacactttt tagcaaactc tataatctct tttagtctag tatattgaca
    39961 atacccctct gcttcaacta atccagcatt ataagctatt tttctattct cttcttcttc
    40021 ataaagttgc tttgcttttt gttgtagttc aatttctcta gttggacata tcttcattaa
    40081 tttatccata tttccattag aacagcctct aagattacat tttccacact tatacatatc
    40141 caatatcccc ttccaaaatt ttattcatct tgcgattctt ctttttctag agttacattt
    40201 tcttcttggg tttcttcttc tggtttcaca tgctcgacta cagttataaa atatgatttt
    40261 cctattttat tccttatagg aagtttttca tatgaaacat ctattttatt ttttatacaa
    40321 gctatatcaa aatgtcttaa ggctacacct aagctaacct tattcatatc ttcacagtgt
    40381 ttcttaggtc tcttttcaaa taaatgaaga cctcgttcat ccttatggaa ataccacggt
    40441 tgagaatttt ttacagatgg agctcttctt gtcaattcta agatatcttt ccactttcta
    40501 ttcctattta tcttatttat catatctttt actggctttc tatcaggtct agcatatctc
    40561 gtcctaaaca actcttcatt ttttgctgga tatccaaatg ctattattat ataaggattt
    40621 tctattttgt tttcatcatc ttcttctgtt tttattccag ctagataatc taaatcatct
    40681 tcctcatctt cttcaaaatt cttattccca atatctacaa attccaagat atcttctctc
    40741 tttaagtttc cttccaacca gcaagtagct attcccaatg ttgtaaggcg caaaactaca
    40801 ccttctgtag caaatcctat attctgtaaa tagtcctcac ctttgtctga tgtaactact
    40861 atataatgag gtgcctttac tttacattcc ttccccatca aaaaatgtat tatatgacct
    40921 ctatcaacaa catgtgcttt aatatttaaa tcttcattta agtattctaa atttttacaa
    40981 cttcttttta tttcttccat caatggcttt tttatctttt tactggaaaa atttcttaca
    41041 gattttctat aaaaaatggc ttcataaagc tccatttatg ccctcctaac tgaaatcttc
    41101 tgacgtataa actctataaa tttatataaa tactttatat tttttatagt tttatttcct
    41161 ttttcctcct gccctatttt gaaattcata agaatatcta ctctagttta atatagcatt
    41221 caaaaggctt aaattctacc ttatcaatgt ctatacatta gcataatttt tttatttgat
    41281 tttctatgct aatttaaata acaacactag ctattacctt tttaattaag ctttgccaga
    41341 aaaatttttt ttcatttata aatatctatc tcattattat attataagtt tttttcggta
    41401 ttcttgtata gcttttcact aaaaaagtat ttttatttct actttaatat tattatcaac
    41461 aaaaaacata ctacttaaat aagtttgtta tcctcataat ttgttttaat taaaatattt
    41521 tttttatttc tcaatctata aagtataaat attttgaata tgacccaaaa tctaagtaaa
    41581 gttataatat tttataagtt gttcaatatt aaaaacataa gtaatgttat aatactttat
    41641 aagttattca atattaaaaa tataggagga taaatatgaa tttaaataga aatttagaaa
    41701 aatacgctga attagcaata aaagttggag ttaacataca gccaggtcaa attctactta
    41761 taaagtcacc aatagaatgt gctgattttg caagaaatgc acttaaagaa gcatataaat
    41821 gtggtgctaa aaatgtttat atagagtggt ctgatgaaga aagtactctt attaaatact
    41881 tatatgctcc agatgaagca tttcatgagt tccccaaatg gacagcagaa caatatgtag
    41941 atatagcaaa agaaggtggg gcatttttat cagtctatgc ccaaaatcca gatttattaa
    42001 aagatgttga ccctgaaaga gttgcaaatt ttcagaaggc atctggaaaa gctttaaaag
    42061 aatggcgttc ttacactcta actgataaat gtaaatggag tatagtctct gtgccaacaa
    42121 aagattgggc aaaaaaagtg tttccaaatt tatcagaaga taaagcaata gaaaaactgt
    42181 gggatgctat atttaaatgt tcaagagtag atggtcagga ccctataaaa gcatgggaag
    42241 aacataatga aaatcttaaa tctaaaatgg actttctaaa taaaaataac tttaaaactt
    42301 taaaatataa atcctcaaaa actgatttga ctctagactt gccaaagggt catgtttggc
    42361 taagtggagc atccaaagac cctaatggaa taagttttaa tccgaatatt ccaactgaag
    42421 agatttttgg aatgccacat aagtttaaag taaatggaac tgtttatagt acaaaacctc
    42481 ttgtttatgg tggaaacata attgacaatt tcttcttaac atttaaagat ggtaaaataa
    42541 tagatttctc tgctgaaaag ggactagata cactagaaaa acttattgaa acggatgaag
    42601 gctctcatta tttaggagaa gttgctcttg ttccatacaa ctcaccaata tccaatacgg
    42661 atataatttt ttataatact ctatatgatg aaaatgcttc ttgccacttt gcaattggtt
    42721 ctgcttataa gacttgtctg gaaggtggaa acaatttaaa agatgaagaa ttagatgaac
    42781 atggtgtaaa tgacagttta acacatgtag attttatgat tggttctgct gatatggata
    42841 ttattggtga gacttatgat ggaaagcaaa tacagatttt taaaaatggt aattgggctt
    42901 tttaacatat gattttaact aaaaagggct gatggaagtt ataccagact tcccagccct
    42961 ttttaatgtt gatgataaat attttttata ttaattaatc tcattattat agattaattc
    43021 ctattgtttt aaacataagt atctaaaact tatatttata ttatatataa aatatcctat
    43081 ttagtaaaac ctttatttta atttatatgc taaaaattta actttgctat ttcaacagca
    43141 gactttgcat cctttgaata ataatcagca cccatttctt cagcgtattc ttctgtcaaa
    43201 actgcacctc ctacaaatac ctttgcattt atctcattat ctctcagtgc ctgaattgta
    43261 tctttcatac tttgaacagt cgtagtcata agtgcactta atcctactaa ttttatattt
    43321 ctctttttag caacttccac tacttcttca atagggacat ctttacctaa atctataact
    43381 tcatatccat aattttctag cattattttt actatatttt tcccaatatc atggatatca
    43441 ccttttactg tagcaactat tatttttcct ttggatacat tattgctact tttacttaat
    43501 aaagtttcct ttattgtatt taatgaaact tttaccgttt ctgctgattg aatcaattgt
    43561 ggtaaaaata tatctccact atcatatctt tttcctactt tatcaagagc aggaattaaa
    43621 atctcatcta atatataatt ttcatccttc tcttttaaaa gattcagtgt tacatttttt
    43681 gcttcatctt ttaatcctct ttcaactaaa atatccaagg gcaaatcttt atcaatcttt
    43741 gtagatgaat ccgatttttt tgaattattt atattgctat attgatttat atagtttata
    43801 catcctttat ctgtgttatt taatatttta aaagaattga tagcttccat catactatat
    43861 tcatttggat ttataattgc taaatcaagc cctgccccta aagctaatgt tagaaaagaa
    43921 gcattaagtg ctttcctatt tggaagacca aatgaaatat ttgaaacacc taatatagtt
    43981 tttactccta gtgtctttac cattgttata gcttttatag tttctattgc ttcttcttgt
    44041 tgtgctgaaa cagttaatga taaacagtct ataaatatat cttttggttt tatcccataa
    44101 gaaactgctc tattaactat cctttttgct atttcaaatc tttcttcagc ctttttaggt
    44161 atcccctttt catcaagtgt aagtccaacc acacatgaac catacttttt aactattggt
    44221 aatatacttt ctaaagactc ctctttgcca tttacagagt ttactattgt tctaccatta
    44281 taatatctaa gtccttgctc taaggcctct acattggaag aatcaacttg taaaggtgta
    44341 tctactactg cttgaatctc tcttattaat tttggcatta gttttttttc atcaatctct
    44401 ggaagaccaa catttactcc aagtatttgt gctccagcat ttacttgttc taaacctaaa
    44461 tttatagcat aatcaacatt ttcatttttt aatgcttctt gtagagattt tctacctgtg
    44521 ggattcaatc tttctccaac aactgtaggc gattgtactt ccacaaattt tgatggtgaa
    44581 cagactacac atttatcaat tttctcgata catccctttg taacagaatt tatattctca
    44641 gatatttctt ttataaaact tggattcgta ccacaacatc cacctattat agtagcacca
    44701 acttcaacga actttttaac cccttcaaaa aattcttttg catcaacatc atatatagcc
    44761 tctccatcaa ctatatttgg aagacctgca tttgcttgaa ccataacagg gactgtagct
    44821 cttgatgcta ttttttcaac tattggtaaa agttgtttag gtcctaatga acaattaact
    44881 cctattgcat ctacacctag accttctata gtagccacca tacattctgg catacatcca
    44941 gtaaaacttc ttccaccttc atcaaatgtc atagtacaga aaataggtaa atcagagttt
    45001 tcttttgctg ctaatacagc tactttagct tcatataaat ccatcatagt ctctattact
    45061 attacatcaa ctccagattt aacgccttgt agtacttgtc ttttaaatat ttcatatgct
    45121 ttatcaaaac ttaaagtacc cataggctct agcatttctc ctataggacc aatatctaaa
    45181 gctacatatc ttggtttagt attatctaca ttttctatag cttttctagc caccaaaaca
    45241 gcattgtcaa tcacttcttc tacagtatac ttactatcta attttagttc attacatcca
    45301 aaagtatttg taagaattac attcgaacct gcctctaagt atgctgtatg tatttctata
    45361 agtttatctg gattttgaag accaaatact tctggatttt cacctaactt taaccctttt
    45421 tgttgaagca ttgttcccat tgcaccatca aatattaata tattattttt cagatattgt
    45481 cttatttcca caagagttcg ctccttttct atatgtacag tttctatagt ttttacaatt
    45541 ttcacacgac ttttctttct tagatttatt tattcttgta ttagaaatac ctataatagc
    45601 cactactgac tttctcggta tcataattcc attagaagta attgttagac ctatttgttt
    45661 ttgagcattc aaaacgttta atatatctat atttttttct attggtaaat ctccatatcc
    45721 tgggctatat cgcatagtta taaatttatc ttgctttaaa aaaacctttt ctaaattatt
    45781 ttgaacaatg tcacacactt cttctattgc tgttgttcca catgaatcaa ttattagtcc
    45841 ttttgttaaa tttgaataag agtgtcgtct tatttctttt tctatatcta tccctatagt
    45901 agctgccata attacacact cattacattc ttttaaaagt tcatatacat ctttactctc
    45961 taatgttaaa tttgttccct ctaattctac aacattatct ttatttattt taataggata
    46021 aatcctagat aaatatctag gatttatttt atttttagtt atagctacac attcatcaat
    46081 agtatattta aggtctttat ctatctcttg acctctatat tgtaaatatc ttaaaacttc
    46141 actttgattt atgcttatat cttctcttcc aattaaatta ttctccatat atatttaaag
    46201 aaaatccttt atctaaaaca tcttttgcat attcctttgc cccaaataaa gataagtctc
    46261 tgtagtttcc acattctata gctgtagctg ctggcatttt atctgattca agaacttttt
    46321 ttaatgcttc ttctaaacca atcttaactg ttttggcatc tacatctccc catagtatta
    46381 aataaaatcc tgttctacat cccattggag ataagtctat tacaccatct aatgtatctc
    46441 ttaaataagt agctaaaaga tgctctaaac cgtgcatagc agcagttcca aaactttcta
    46501 catttggctg taaaaatctc aagtcaaatt tagttacttt atctcccttt actccatcaa
    46561 gtacactaca ctttcttaca aaaggagctt ttacttttgt atggtctagc ttaaaacttt
    46621 ctactttttc cattatttaa tcctccatta tactttaaaa tttgttacat taattctaaa
    46681 aaaaactctt cctatctatt attgataaga agagtatatt atttcaagtc atctcatcgt
    46741 tgttagcagg accacctgat aaaaggttgg tataggatca ctgagctaac tctctcccct
    46801 aactcttgat aagtatctta ttaaactgac ttaaattata tattactttt ttatttttgt
    46861 aaacacttta ttgttataat atttatatca cataatcgta cttttatttt ataactaagc
    46921 tgattgtatt attggcagtt gtacttcaaa cacagtttga tatttatcgc ttgaaacagt
    46981 aatttctcca tcatgtaaat ttacaatatt ttttgtaatc gcaagaccca atccagaacc
    47041 tccaacattt ctatttctcg atttttcaac tctataaaat ctatcaaata tatttggtaa
    47101 atctaacact ggaattggtt ccccataatt tatcactttt acaaccgcca tatcttcctt
    47161 tttctctgtg actatatcta cataatgccc atcttttcca tatttcacag cattagttat
    47221 aagattttca aatgctctta ccaatttatt tggatcagct tttacaatta atttttcttc
    47281 attaaaatta attctactaa ccataaattc ttgtttaaaa taaatttcaa attgagatac
    47341 tacttgactt aataactcta ctaaatttat ttctattttg tttaatttta ttgtattatt
    47401 ttgcatttta gtcaattcaa acaaatcatt tataagaaca tttaaatttt tcgatttttc
    47461 atatgcaata tcaacataat acatcatctg aacttcatct ttatacttac cttcttctat
    47521 cactcctaaa taacccatta tggatgtcaa aggtgttctt aagtcatgtg acacatttgt
    47581 tattaaatct gtttttgtct gctgagcttg tctctcttca atagttatat ttctaagttg
    47641 atggactata ttattgattt tatgtgctag ttcatcaata tcatcctttt ctttatcctt
    47701 taatatatct aaatcatact caatatctat acttttttct aagttacctt catccattaa
    47761 atttacagca cttttaatga tttctaattt ttgatttttt ctattagtct ttcttataaa
    47821 atctacaatt tgcttatttc tcttgtacga tatcatggaa taaattgcta atgatgtgat
    47881 tgtaaataaa aatggataaa ataaatcata ccctataaga cttctgaaat ctcttgcttg
    47941 atatacaagt tgattaacta cagacatatc actattgaca tataatgatt ttattatata
    48001 catacttgtt aaaataagca ttgaagttaa agcacagctt attataagat ctaatatggg
    48061 tcgaaacata tgatttaata aacttttatt tttcaatttt atatcctact ccccatacag
    48121 tatgaattat tttttctcca tctgtatatt tttcaatttt atcccttatt ctgctcatat
    48181 gaaccattac agtattatta gattggtaat atttctcttt ccataccttt tcaaatattt
    48241 cttctgtact atatactctg cctttattat ttgctagtaa atataatata tcaaattctc
    48301 ttgatgtcaa aattatttca tttccagcca ctgttacact atgtttttcc ttatctataa
    48361 ttactactcc aacctttatg acatctttat cttcattatt agcaccacta aaataatatg
    48421 ctcttctaat cagcgacttc actctcacag taagttcaag tgggttaaat ggttttgtca
    48481 aataatcatc tgcaccagtc attatccctt gtattttatc catgtcttct gattttgcac
    48541 ttaacataag tattggtata ttcaaacttt ctctaacttt tctacaaact tctatcccat
    48601 ccattttagg catcattata tccaatatca ttaaatgaat attttccttt tttaaaatat
    48661 ctagagcctc ttttccatct tcagctttaa ttacattata tccactattt aaaagatata
    48721 tctctataag gtctcttatt tccttttcat catccacaac aagaactgta ttattcattt
    48781 ttacacatcc taacttaatc tttgtatata gctttattca tataaaatta tactatattt
    48841 tagttagtgt aatctataaa tttattaatt tctaaatttc caatataata caaattattt
    48901 acacatttat attatttatt ttgtaaatat tcttctaaag ttaatccttt tgaataaatt
    48961 tcctcggcag catccttacc aacatatcta atatgccatg gttcatatgc tgtacctgtt
    49021 atatcttctt taccttcagg atatcttatt ataaatccaa acttgtgagc attttctgcc
    49081 aaccatttag cttcttttgt acttccaaca aaccatcttt ctggatttgt taaatcaata
    49141 caaagtccag tttggtgctc actgtaacca ggctttgcta catacgcatc tgccttttct
    49201 cgcccctgag attttaccct tcttgtataa gtatccagtt gagtatcata agatctgtat
    49261 gctgaagacc ctaaaaactg tattccttct gaattagctt gcttaactaa atcttcaact
    49321 gcctttgcag gttctcctgc catttgtttt tcttcctctg ttgcttcttc aacaaaaggt
    49381 atgtttacct tagtaatatt ttcaggagta tagtttttgc taattccatt tgtcttgttt
    49441 accaataata tatttgatat actatttgag tgatttttat tttgagaatt tgcctcaaca
    49501 tcttgttctg aatcatgttg agcagctact attggatctt tatctttctt aagtaatcta
    49561 tctttagata taaatacact tataaatact actaaaataa tggctcctat taatttaaat
    49621 tttttcataa ttaaatcttc tcctttttgt tatgtttgct ataatgttaa ttttagagta
    49681 taagacttaa gtattttata agtaaatctt aaataaatct taaatttaat atttttaaag
    49741 gatggtgata aaatgtctat acaaaaactt ctaagtaaag ctcgtaaggc tatacaagat
    49801 tttgatatga tacaagaaaa tgataaaatt gcggttggac tttctggtgg aaaagatagt
    49861 ttaactctac ttcatatact taaaagttat caaagatttt ctccacaaaa ctttgaattg
    49921 atagccataa cattaaatac tggaggtgtt gataattctc cactagataa attgtgtaaa
    49981 gaaataaatg ttccttttta tgaatttcaa actgacataa aagaaattgt atttgatata
    50041 agacaagaaa agaatccttg tgcattgtgt gctaatttaa gaagaggtgc tttgaacgat
    50101 aatgctaaaa aacttggatg taataaagta gctcttggtc atcataaaga tgatgcaatt
    50161 gagacatttt taatgtctat gttttatgag ggtagagtaa attgtttttc cccaaagaca
    50221 tacctagaca gacaggattt aacagttata agacctatgg tttacataga agagtatatg
    50281 actaagaaaa tttcaaaaga ttctaactat cctattatca caaatccctg ccctgcaaat
    50341 ggtcatacta ggagagagta cataaaaaat cttattgcta atttaaataa agagatgcct
    50401 gattttaaaa gaaatgtatt tggtgcttta aataattctg agaaattgtt tatatgggat
    50461 aaagagaaga taaaaaattt taaataataa ttaaaatttt taaataaaaa agcaagattg
    50521 tattaattgt aaataatctt tatataatct acacaatctt
//
LOCUS       DAMJVK010000016        50543 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.016, whole genome shotgun sequence.
ACCESSION   DAMJVK010000016 DAMJVK010000000
VERSION     DAMJVK010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 50543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50543
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.016"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            125..967
                     /locus_tag="PC414_000944"
     CDS             125..967
                     /locus_tag="PC414_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HDF2463353.1"
                     /translation="MKEKAGIRNAIIAAFIITILAELIGPISFKVMGINVTLLSILWA
                     IFIGMAVSPHLLGRVIPALKKFIGDNEINVSPYLLSLTLYPLGIMFGINAGPKVGILL
                     QAGPALLLQEFGHMGTMLIALPVGVALGLGRSALGGTFSLCRDTALGIIGDKYGLNSP
                     EGIGTLGTYISGSIFGTLLFSFLAPVGVLLGFHPYALAMASGMGSGSMMGAATASLMN
                     TVPQMSEQILAYSATSGLITAVTGIYIELFLSLPVANYYYSKVAPVIERFRGRKTRRT
                     SNKL"
     gene            982..1446
                     /locus_tag="PC414_000945"
     CDS             982..1446
                     /locus_tag="PC414_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463354.1"
                     /translation="MEKTFKTIMEQIKIVLFIYIIICFGQMIALKTQAQDLLIGSVSG
                     FLLVIIAIIIKNFVKKPNLPGFAWATLVAFVLSLPISPVGDIIATNVGKINFMSTVTP
                     LLAFAGISVGNQLDILKTLSWKLVVISFIVMTSTYFGSAFISQTVLRLNGML"
     gene            1433..2605
                     /locus_tag="PC414_000946"
     CDS             1433..2605
                     /locus_tag="PC414_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2463355.1"
                     /translation="MECFKEVVNMNELKAKIKEEIKLLSEEKKTSFEKVSDYLFSNPE
                     LAFEEYKSQKALCDLLEENEFNVTKGVGGLETSFEAVYSNGTNGKTVAFLAEYDALPG
                     MGHACGHNIIGTSSVGAGIILKEIMKKHNIEGTVKVFGTPAEERVGGKITMIKEGVFN
                     NVDAALILHPSDASMPDDISFAQVNLKFDFTGKASHAAAFPWEGKSALSGVIALFNSV
                     NSMRLHLKDYARVHGIITDGGSIHNIIPEKSTAIFNVRALSIEYLNEICEMLKNCAKG
                     AAISTGTNVEIVQLDEIYKEIKNDSELVNIVRQNFEVLGEDYVERDLSQGIGSTDTGN
                     LTHEIPAIQAYIKLKENTATHTDEFAVAAGGEEGRVALIKAIKVLAMCGVDILYSK"
     gene            2649..3584
                     /locus_tag="PC414_000947"
     CDS             2649..3584
                     /locus_tag="PC414_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1177 domain-containing protein"
                     /protein_id="HDF2463356.1"
                     /translation="MILKQVIEVYDILDKANANGEEVKTYLQGYGEVDVTVKELSSSK
                     GSTDLVKLTIPGKNGKLKGGDAPTLGILGRLGGIGARPEVIGFVSDGDGALVAIAVAA
                     KLLDMQRKGDILDGDVVISTHICPDAPTKEHYPTPFMDSPIDMMTMNENEVDSSCDAI
                     LSIDTTKGNRVINTRGFAISPTVKEGYILKTSDNLLDIMQTVTGKSPFVFPLSIQDIT
                     PYGNNLYHLNSILQPAVATDAPIVGVAITTEMPVAGCATGATNFSDLDSAGRFAIEVA
                     KLFGRNKCDFYDKEEWEMLIKRYGKLNRFQTFGIQ"
     gene            complement(3858..6551)
                     /locus_tag="PC414_000948"
     CDS             complement(3858..6551)
                     /locus_tag="PC414_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HDF2463357.1"
                     /translation="MNNDNDKNGNKIRRKKVSSSGNTNKPINRTSANKTRSTKNKKKS
                     KKSDKFKKLRVFGIVFLVLLVVGTAGTAGLVFASLRDVTPVTEAVLDKQTNQTTTIKY
                     ANGKTLSTAPSVNKKTPVPLDKISPYLQHAVIAIEDERFYEHKGVDIKGLFRSVLKTL
                     TGTKQGGSTIPMQVSKMLLTTEQQTIPRKIKDIYYAHEMSKTVSKDKILETYLNNFFV
                     GRGLAGAEAGARGYFDKSAADLTLAESALLAGSTKNPSRFSAYKTSKLEGNETKEDLE
                     NKLLFFVNTTDDDLDDPTQVDFDMIEKIKSWELISNDTYRQLKAGTLVVRKAVSNPEA
                     KKRQEIVLKKMLELKYIKQSEYDEAIKAKIEIKLPQSSDKVSSSVEDLIESEVINALM
                     EQGHTNDEAQNLFYNGGLIVNTTIDPKMQDALEEEFDRNSNFPGHMVGPDGVSQPQAA
                     MVILDYKNGEIRALAGGRNISGRKTLNRATNPHQPGSSIKPLAIYTPAIDTLKITQAT
                     ALSDSRGGYKFEENNKWNPRTTTAGHGSMSLRKALAKSSNTIAVKTAEMLGDSYDECV
                     DIMMDYLKNFGITTLKNNHSGSSEASDRKFPSLTLGGMANGITPLQMAAAYGTLANHG
                     IYVEPSIFTTITTFDGQLLVKNAPEEHKVVDPEVAYVVTDMLESVITEGTGGVATLPK
                     GMPVAGKTGTTNSAYDAWFVGYTPYYVGATYIGDDAGRKDDSGNTIKRREVPHGSTST
                     AKLWEKIMEKIHANLTVTEFEVPKNVYFTKINLEDGGKQSSGSKAAFIEGTAPTKVSS
                     QPSSEDTKKPDNNQPEENNNNNNNTGDNGGGSTPPDNGGNNGGGSTTPPDNGGNNGGG
                     STTPPDNGGNNGGGSTTPPDGGGNNGGGSTTPPDGGGTPAT"
     gene            6765..7463
                     /gene="yunB"
                     /locus_tag="PC414_000949"
     CDS             6765..7463
                     /gene="yunB"
                     /locus_tag="PC414_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YunB"
                     /protein_id="HDF2463358.1"
                     /translation="MNRIVDRKTKNEFRKIIAIFLVILFLSVFIGSFIYIDKTLRPTI
                     TVLAETKALELANRSINKAVAEMVEGKINYEDLMDIQLDNNGKITMIQANTIMMNEIA
                     SAIALEIQDELKKDKTASSYIPIGTALGSPILAKYGPKLEVSIEPIGTVSVNFKTEFE
                     SSGINQTRHRIYLEAQTQVKVVIPLITSTKQIKAQIPICETIIVGDVPESYVNIPEKN
                     LGNVLPNTGKNTNK"
     gene            complement(7675..8682)
                     /locus_tag="PC414_000950"
     CDS             complement(7675..8682)
                     /locus_tag="PC414_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HDF2463359.1"
                     /translation="MQLKNFHFKNNTLSKKTNLAKSKFTIVLTFLFLLYFFSNNLIYA
                     QNLQKNEPEYLIPIGNVLQIDAELKNIIVRNPGEASPFRLGDAIVKVNNAEVDGYSDF
                     ANTLNALPEEKQVSILLNRGGQLITMKTTKHILEKISFNNLLSGFATLTYINPDTSEF
                     GAVGHPISVGNARKIPIKTGSISTTKHLNIEKSYKGSVGCINAKRENIIGSFTENTDF
                     GIRGQINNFDISKLKKYKVASLDEVKLGKAQIILQDNFNKCKKYNIEIIDIENQKHPE
                     SKSFKIKITDKKLLAQTGGIVQGMSGTPIVQDNKIIGAVSHAIENDPAVGYGVFIKWM
                     L"
     gene            8910..9794
                     /locus_tag="PC414_000951"
     CDS             8910..9794
                     /locus_tag="PC414_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2463360.1"
                     /translation="MKKYRLLVTCIIAMGIIIIGTSNPVSNFYKTTNLISSGNITNEK
                     ADKDENSNKNNKDKNEDKSNSKSNKDKSSEDKSSDNKNEIQKNKKLLICIDPGHQGKG
                     DSNLEPVAPGSSSKKARVSSGTEGIATKKPEYVLNLEASLVLKSILESKGYNVIMTRE
                     THDVNISNSERAILANDKKADMVVRIHADSLNNSSKTGASILIPEKDGKYTAPIYEES
                     NKCAEFIKQNMEQSGIQINGIVQRGDLTGFNWSKVPAVLVEMGFMSNYNEDQMMSNPD
                     YQRKMMQCIADGLDAYFK"
     gene            9996..11546
                     /locus_tag="PC414_000952"
     CDS             9996..11546
                     /locus_tag="PC414_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDF2463361.1"
                     /translation="MLVVENVSHGFGARTILENVSFRLRKGEHIALVGANGEGKSSFL
                     NIITKKLMPDAGNIKWSSRATVGYLDQHTVLSKGKTIREVLREAFKHMFDLEQEMIAM
                     YDKMGEASDDEMSKLLEETAEIQTILENSGFYMIDAKIQEVANGLGLGEIGLDKDVTD
                     LSGGQRTKVLLTKLLLENPTILILDEPTNYLDEEHITWLTKYLQEYENSFVLVSHDIE
                     FINNTCNVIYHMENGELNRYKGNYDEFVRLNDIKKRQEEQAYDKQVEERKRLEDFVAR
                     NKARVATRGMANSRQKQLDKMEILERPKEKIKPTFAFKDARAASKIIFETENLVLGYD
                     EALTKPLNFHLERGKKIALKGMNGIGKSTLLKTLLGIIKPFEGNVKLGDYLEVGYFEQ
                     ESSRENSNTPMDEVWSEFPGLTNFEVRQALAKCGLTNEHITSQMRVLSGGEAAKVRLC
                     KVMLKNINFLVLDEPTNHLDVEAKDELKKAIKEFKGTVLLVCHEPEFYSEIVDDVWNI
                     EDFTTKIV"
     gene            11575..12222
                     /locus_tag="PC414_000953"
     CDS             11575..12222
                     /locus_tag="PC414_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HDF2463362.1"
                     /translation="MKKFLNVLFSICISIVIIVGVINFTVGFKQLYYFDIDYLNISEL
                     SGLSKDDIKLNYDYLIDYNLNKNVSEFKLPTLKSSPQGKIHFEEVRNIFQNINKLAKL
                     LLVVSLVGIILSVKNKNIKILKTTSITLIIMPLLLTVPILLNFEKSFIIFHKLLFRND
                     YWIFNPDLDPVINILPEEFFFHSGMMILILILLVSIVLFVMYKLCKSLSIKIFQC"
     gene            complement(12364..12624)
                     /locus_tag="PC414_000954"
     CDS             complement(12364..12624)
                     /locus_tag="PC414_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich small domain-containing protein"
                     /protein_id="HDF2463363.1"
                     /translation="MGENYKFFNHKDCEFFPCHKTNKPEEFNCLFCYCPLYALGENCG
                     GNFRYTDKGIKDCSSCILPHKKDNYNYIMSKFQDLVKITSKK"
     gene            12841..14124
                     /gene="hflX"
                     /locus_tag="PC414_000955"
     CDS             12841..14124
                     /gene="hflX"
                     /locus_tag="PC414_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDF2463364.1"
                     /translation="MKEIQEKALLVGLNLTTIVKKNDDIDTNESMEELKELTKAAGAE
                     VVGSLIQNKHSVDAAYYIGKGKVEEIRAYSDSLDATLVIFNDELSGAQIRNIENVVGR
                     KVIDRTTLILDIFAQRALSKEGKLQVELAQLKYRLPRLYGMGGEMSRTGAGIGTRGPG
                     EQKLEIDKRHILNKAADIRRELKEVKKNRETQRVKRLKSNIPIVALVGYTNAGKSTLL
                     NELIKTHKDYEQEKEVFVKDMLFATLDVTLRKALLPNKKEFLVVDTVGFVSKLPHDLV
                     EAFKATLEEVQYANLILHVIDATNTSYELQKSTTEGVLKELGVNDKKHILVYNKVDKL
                     ELDIYPKSQEDIVYISAKQGINMDKLLNMIEIALMENTYSVSLMLPYERGDIFSRIKD
                     KYNVENFEYGESGITLDADLDEEDFNIYREYILEK"
     gene            14132..14809
                     /locus_tag="PC414_000956"
     CDS             14132..14809
                     /locus_tag="PC414_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HDF2463365.1"
                     /translation="MDINWENIDNLEDYFITYLLYKESKTVSQISKIRNISSTEASEQ
                     LIQAKLKIKEMQKDDFEASKDILDKFLELDKIKRLDFMDSLDDEKMVYFKRKVFKRIL
                     VEKNAEDLIVLIWATGELKDDRFLKLLHQLTNHRHSDIRRITYSAIRKIESPSSREVL
                     QKGLYDKNAQTRQYCAKALSKLGDENSLKILQQLKDKNKNFEKEYVLRAYDEAIKSLE
                     NAKNLKK"
     gene            14895..15338
                     /locus_tag="PC414_000957"
     CDS             14895..15338
                     /locus_tag="PC414_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDF2463366.1"
                     /translation="MIVRRCAGGVVFYANKVLIVKNDRGEWTLPKGKILGGGLPYESA
                     VQRVKVETGIDAKMIDVAGDTMYEFFSRSRQQEVCNAIMWYVMEACNTDCVLAPEFQE
                     GGFYKVKDALEMLSHHKEQALVEVSYKKFKELKKLVSEGDDVPAN"
     gene            15643..16287
                     /locus_tag="PC414_000958"
     CDS             15643..16287
                     /locus_tag="PC414_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HDF2463367.1"
                     /translation="MSNYRTLHEFGTDEITIEKSVFIGYAKPIKSEEEALEFINEIKK
                     KHKDATHNVWAYTVGKNMNIQRYSDDGEPQGTAGIPTLEVIKKEDLRDVAVVVTRYFG
                     GTKLGAGGLVRAYTKGAKLGLEAGKIIYKVMYQEVKVKIDYTQLGKVQNELMNLGYFI
                     KDTVYEDNVEIVVYSRLEDVEKLSEKMIDITSGTGKIVLGEEFYLSEQNGEILI"
     gene            complement(16373..16750)
                     /locus_tag="PC414_000959"
     CDS             complement(16373..16750)
                     /locus_tag="PC414_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDF2463368.1"
                     /translation="MLFALIGFFAGIIGGMGMGGGTILIPALVLLAGIDTKIAQSVNL
                     LSSIPMTLIALAIHIKNKNVIFKLVIPIAVFGVLGAVFGSLLANYLSSDILKKIFGIF
                     LLLVGLFEVKKGFCAKSDKKPLN"
     gene            complement(16750..17136)
                     /locus_tag="PC414_000960"
     CDS             complement(16750..17136)
                     /locus_tag="PC414_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDF2463369.1"
                     /translation="MKKTDDLKKKYFDFNLKNTIIGVFTGFINGVFGSGGGTLLVPIL
                     NDIVKVEEHKSHATALSIIIFLTTASSVLYVSKGTYDVNLTIKVAVGSILGGIIGAKL
                     LNKVTGKFLRISFGIIMIIAALRMVF"
     gene            17315..18070
                     /gene="nadE"
                     /locus_tag="PC414_000961"
     CDS             17315..18070
                     /gene="nadE"
                     /locus_tag="PC414_000961"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HDF2463370.1"
                     /translation="MSNIKIQIDKTVEWLINKVNEANAKGLIVGVSGGIDSAVVANLI
                     KKAFPENSMGVIMSIKSNPQDREDALKVIEGCDIEYLDLDLIEPQSAILDMVVGNLKD
                     KHLYREEYLKMTDANLRARVRMSTIYTIANNLGYLVVGTDNAAEIHTGYFTKFGDGGV
                     DILPIANLTKGEVYEWAKELGVHEDLINKAPSAGLWEGQTDEDEMGTTYNMIDAVLEG
                     RLDEVPKRDQEIIERLHRLSEHKRKTPAQPPKF"
     gene            18153..18872
                     /locus_tag="PC414_000962"
     CDS             18153..18872
                     /locus_tag="PC414_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDF2463371.1"
                     /translation="MGRIGNIINRKGKQDAQRAKIFTKHARAIAVAAKEGGADPEYNA
                     ALKTAIEKAKADNMPNDNIDRAIAKGAGAGAGEDYETIVYEGYGPGGVAVIVETLTDN
                     KNRTAGNVRYYFDKNGGNLGTSGCVSFMFDKKGQILVGLGDGVSEEELMDVALEAGAE
                     DFITEEDGYEIITTPEDFSSVRDELKAKGYEFISADVKMIPQTTTVLTEESHLKMMNK
                     LVDMLEEDDDVQDIYHNWEVE"
     gene            complement(19039..20043)
                     /locus_tag="PC414_000963"
     CDS             complement(19039..20043)
                     /locus_tag="PC414_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDF2463372.1"
                     /translation="MATIKDIAKDCGLSSATVSYVLSGKGNERRIPLHTQELILNSAD
                     KLGYKRSTPIKHNNIKIAFYWQDEGFELASTNILQAINHIISLEHLNINIIIRPYKVG
                     HLCDDEYFFANNYYDGIVLTGINADDIEYLVKNKPKIPVVVVNREIPGFSSVSIDNEE
                     TGRIAALHAIQNGKNDIAVVFNPTSFTCMTLRGKSFLKTCKEHGIHLSDSIYYCSNKI
                     DSGYDIAQKMLAKNSLKKVIFCNHDVVALGMMNAFHESGIVIGEDIQIFAASNGPSRL
                     MARSIPPLTVIDLKMEETAERSLRMIIDTVTGRINGIQQQVIYPSIIYRQSSPAQNLL
                     "
     gene            20309..21205
                     /locus_tag="PC414_000964"
     CDS             20309..21205
                     /locus_tag="PC414_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HDF2463373.1"
                     /translation="MSKVNIFEVGPRDGFQNLKEFLETDKKLKIIDGLIASGIKQLEI
                     SSFVSPKAIPQMKDAKEVATYCVEKYPDVKLYALVPNLYGAKVAWECGIRDISYVISL
                     SETHNKKNINRTHEESIDELKKIMNTYPDMNICIGMATVFGCPFEGLPKIEDAIRFAK
                     RLWENGIREINLADTIGIADPKQVRETISALVEALPECRWQVHIHDTRNMGMVNTLVA
                     IESGITTVQSTLGGLGGCPFAPGASGNTATEDLVYMLERMGYDTGVDFVKLLELSKYQ
                     KSFVNGVYSGHHVNIKSDSCNC"
     gene            21326..22588
                     /locus_tag="PC414_000965"
     CDS             21326..22588
                     /locus_tag="PC414_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463374.1"
                     /translation="MDIIALLLFLGVIIIAFVRKNNAGILALAIGVIAVRIFGMDDKE
                     LYGAISNSMFTTLVGITLLFAIVNSTGALKLVAEKIVAASGKRVWLIPIAVFIAGFVV
                     AGVGPGAIPALAIIPAMAVPVAITVGYNPVMLALIGECGLMAGRMTPITPEGQIITTV
                     AEGVGIENVMPTILACQTLSTIVFSIVLFIIFKGYKLKKPINVIDSKQIEKFNIKQII
                     SLISIVVMIAFIIIFDMNVGLAAFAVSAVLIFFNIADEGECIKSIPWSTIVMVLGVGA
                     MMTIVDAAGGIDLMSNALSSLMNSKTATPIMSISAGLMSMVSSALAVVYPTMMPMCVD
                     IAKAVGGVDPLALIAAVGVGGSLAGVSPLSTGGALILAAMGSSKKDFNKEEQNKVFVQ
                     LFIMSAVGLLVIAVVSILSFNAITHLMN"
     gene            22664..23857
                     /locus_tag="PC414_000966"
     CDS             22664..23857
                     /locus_tag="PC414_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HDF2463375.1"
                     /translation="MKKGALSNIKVLDLTRVLAGPYCTMMLADFGADVIKIEMPRKGD
                     DTRGFGPFVNGESLYYANINRNKKGITLNLKSEEGKNIFLEMVKHADVVVENYRPGVM
                     EKLGLGYDELAKVNNQIIYGAVSGFGCYGPYSERPGYDIIAQAMGGLMSITGQEGGEP
                     TRVGNAMGDVLGGMNLTIGILTALNARKIIGKGQRVDVSLVDSVVSSLETGIQRYFDS
                     GQPELMGNRYASAYPYDSFKASDGSFVIGCGNDKLFEKLCKKVLFREDLLIDERFKTN
                     LNRCEHYKALKPEIEKWSVKHTIKEAVDLISEAGVPAAPIYNLDCVTTDEHIAKAREM
                     IVDISHPIIGNMKVNGNPVKLMTTKAKIDMPAPSLGESNEEIYNGWLGFDSDKIKDLK
                     ENGVI"
     gene            24486..25280
                     /locus_tag="PC414_000967"
     CDS             24486..25280
                     /locus_tag="PC414_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HDF2463376.1"
                     /translation="MESVMENLNNLKVEVKDKVCVITINRPKALNALNSDTLRELSQV
                     IDVVSENEAILGVIITGEGKVFVAGADIRQMQNYKSEEGRKYAGYAQGIFDKIEALEK
                     TVIAAVNGYALGGGCELAMSCDIRIASEKAIFGQPEVNLGVIPCFGGTQRLSRLVGTG
                     IAKELIFTGRQVNAEEAKSIGLINKVVPSDLLLEESMKMMNQIVEKAPIAIRYAKVVI
                     NKGIDMDLKNALELEKDVAGLTFATRDKQEGMNAFIEKRKPVFENK"
     gene            25375..26715
                     /locus_tag="PC414_000968"
     CDS             25375..26715
                     /locus_tag="PC414_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDF2463377.1"
                     /translation="MNTGVIISLVGIIIALSLLVVMVMKGINIFIIAIVCSSLVAITG
                     SLNVYDALKVDYMTGFVGFLQANFFIFLTGTLMGKIMEITGGAKSIAKMIVRWIGKDK
                     ALLSIPIACGILAYGGVSVFVVSFAVFPIALEIFKEADLPRRFIPAALTFGCSTFAMV
                     APGAPQIQNIVPASTLGTDIMAGAVNGFISCAVMFIVGSTILYKMVSKEKANGGHFIA
                     HESDIFDEAEATNSKRENGPNGVIALIPLIVSILIINVKVNGKAIVPIEVGVFIGALL
                     VYLLLNKYQDNTKIVGHVGDACKTTVVAICNTCAVVAFGSVVKSAVGFDFIVNAMTNI
                     PGPPIAGAAVGTTVIAGICGSASGGLGIAAPLLGPVFLAKGVSAAALTRTMAISSAAL
                     DSLPHNGYIVTVTNGLCKETHKDAYMPIFWVTVLTPFIGTIVAVLLFTMFPGLP"
     gene            26852..28084
                     /locus_tag="PC414_000969"
     CDS             26852..28084
                     /locus_tag="PC414_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HDF2463378.1"
                     /translation="MLNKPLEGVKVIDLTYFVAGPGTSKILADWGADVIKVEPSFGDP
                     GRKTGATMTMPIDDYNNPFYSTYNSNKRGLSINLKSETGIEIMDKLLSEANVFVSSYR
                     TGALKRLGLDYESLSKKHPHLIWGQINGFGDFGPAKDNAGFDTVAFWARSGAMLDIAE
                     KDTSPINPPIAFGDATTSCSLSGGICAALYQQAKTGKGQKVMASLFGQAIWNASSLLA
                     STQFSDEYPKTRKNAISPVINSYRCKDGKWIFLSILEHERYFEALCRLFGREDLIKEE
                     KFASSVESKKNATELINILDGEFAKFTQNEMVEKLTAADIAHEKIQHVNDVVTDEQAI
                     ANNYIYEHTSKDGNKTMLASTPVKFGNIEVNMTCDAPLIGEHSDEILRELGYSDSDIE
                     TLIESGIVTMNKEACEIC"
     gene            28124..29266
                     /locus_tag="PC414_000970"
     CDS             28124..29266
                     /locus_tag="PC414_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HDF2463379.1"
                     /translation="MDFRLTEAQLMLQRVAKEFAENEIAPIAAETDKTGIFPRELFSK
                     MAKIGFNGIGTPVEYSGSGGADIEKVIVVTEIAKKCAASAAILSIHTIYAQAILKFGT
                     EEQKKKYLPMMAEGGCVGAFALTEPNAGSDAARAATTAIIDEETDEYVLNGTKCFISG
                     GGQAESLIIFALTDPSKGIKGMSAIIVDKGTPGFSIGKIEEKMGIHGSETAELIFDNC
                     RVPKSNLLGKEGKGFNIAMTCLDGARIGVGAQAVGIAEGALEESIKYSKERVQFGKPI
                     SALQGIQWYIADMATMVESAKLLVYYAADLKARGEKHTKEAAMAKYNASRTAREVTNL
                     ALQIHGGYGYMKDYPLERMYRDAKITEIYEGTSEIHKVVISRAVLG"
     gene            29277..30071
                     /locus_tag="PC414_000971"
     CDS             29277..30071
                     /locus_tag="PC414_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HDF2463380.1"
                     /translation="MNIVVCLKQVPDTNEVKINKETGTLIRDGVPSIINPDDRNALEE
                     ALKMKDELGAVIKVISMGPPQAKSALKEALAMGADEAYLISDRAFGGSDTWATSTIIA
                     AAIEKVGKYDVIFCGRQAIDGDTAQVGPEVAEFLGIPQVTYAKEVKVQDDKLLVTRYT
                     ETGDYLIEAKMPVLLTAIKELNNPRYPSVKGILEAYNNGDAKITVLTLADLDVDTTQI
                     GLKGSPTNVYKSFVPVKDKHNEIIEGINKKEKAEKLIEILFDLKLV"
     gene            30082..31104
                     /locus_tag="PC414_000972"
     CDS             30082..31104
                     /locus_tag="PC414_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDF2463381.1"
                     /translation="MMRAKVNQGINLNDYNGVWVIGEQREGKINPVTIELIGEGRKLA
                     DQLGKELAVVIAGYEVEKEVKELLHYSVDKVYYINDPLLKDFTTDGYAISIANLIERK
                     KPEVVLVGATSIGRDIAPRIAGKVGTGLTADCTKLEIDSTDNKLLQTRPAFGGNLMAT
                     IVCPKNRPQMSTVRPGVMAKAVRNESETGILEVVTPELTEKMIRTRLVEILPQEKKSV
                     NLTDARIIVSGGRGLKRAEGFELIKELADKLGAEIGASRAAVDSGWIEHSHQVGQTGT
                     TVRPELYIACGISGAIQHLAGMSDSKYIVAINKDAKAPIFSICDYGIVGDLYEIIPEM
                     IESLNR"
     gene            31203..32579
                     /locus_tag="PC414_000973"
     CDS             31203..32579
                     /locus_tag="PC414_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HDF2463382.1"
                     /translation="MKEQWYKDIFARVLSMTDDGFIVVNTSGVIIDINDKYCDFLGKE
                     RKDIIGQNIQSIIPNTKMLDVMKNKYCEEGAIHHYSGGNTKEKSVIVSRSYVENDNGE
                     VVAGVAQVKFRLQSFDVAKKLMSEYMELQYYKEQFKDNCGFDKLIGENRDFIELKKTG
                     VKASKTNFPVLLTGETGTGKEVFARAIHNNSSRSDKPMVSINCAAIPEELLESELFGY
                     DEGAFTGAKKGGKKGKFLVANNGTIFLDEIGDMPLTMQAKLLRVLQESEIEPVGGLKT
                     IKIDVRVISATRKNLSKMVEEGLFREDLYYRLNVINIHMMELKDRQDDILLLANYILN
                     KLNVEYKELKVLSDKVKNCFINYTWPGNIRELQNVIKSAYAVSDDMVIMMCDLPSKMD
                     NISRVAQCNVDSNCSIHEMVENYEKSLIIDVLRKYNWKCSKAAEVMGIHKSLLYKKIK
                     KYEIELNN"
     gene            complement(32911..33255)
                     /locus_tag="PC414_000974"
     CDS             complement(32911..33255)
                     /locus_tag="PC414_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463383.1"
                     /translation="MDQIKRLHELQQKSYLTKEEFEELKNNTYIERFELRDECDNSYI
                     YTFYTNDKCNDIKFIKSQFIVTLIKIGFLCECGGVFRQKKIVDEYDYGYNAIYKCDSC
                     GKELDKEVENYD"
     gene            33743..34753
                     /locus_tag="PC414_000975"
     CDS             33743..34753
                     /locus_tag="PC414_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HDF2463384.1"
                     /translation="MHEDKNDIFESIDTEDFDNKTEEECLELVRKNGLNLIYIKNQTE
                     EICLEAVRQNCNAIKCVQNQTEKICIEAVKQDWRMLEFVEEPTEEICMLAINQDGTAL
                     KYIENQTEELCLRAVEKNGAALEYVKEQTEEICIEAVRNSEFGLARVKNQTEKICMEA
                     VKHCSYNLKWVENQTEEICMEAVRQNGLDLKFVKNQTETICLRAVRQNGMALEFVKEQ
                     TVGICLKAVRQYGMALKFVKEQTEEICTEAIKQDKRALSFVKGDKEKYKALYDNNEPF
                     AKRYVRNVIEKENRALIKKGEENAKIKIAGKLYDRGMSFEDISDIVEIEISKLKVSLG
                     VF"
     gene            34889..35377
                     /locus_tag="PC414_000976"
     CDS             34889..35377
                     /locus_tag="PC414_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2463385.1"
                     /translation="MKITVLYQTRSGNTERVAKLIEEGAKKVDGVDVKLMRLDNIDLD
                     YLNESKAVIFGTPTYLANTTWEVKKWFDEDSKKVNLAGKLGAVFATCDYICGGPDVAI
                     LTIVGHLMVKGMLVYSGGGSLGKPFIHLGHVHSIEGPELQDEKAIIFGERIATKAKEL
                     FA"
     gene            35553..37481
                     /locus_tag="PC414_000977"
     CDS             35553..37481
                     /locus_tag="PC414_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2463386.1"
                     /translation="MVWHIEYEIFSAIIVIFLMVYFFRSKFIPTLQNKIYCALLIFSF
                     LFIISNILGSFCLNNIDKIPIFITFLLNQIYLLLLPIPAALVSFYVMAIIYQDIRYMK
                     KRLLFLSIPLIICIILSITNPFTHILFGLSKETGYIRGCGYVATFASFFFYAIYSAFL
                     SFKYRKAMHESKIWAIRGFLIVSIVAIILQAIFLQYLLTGTACACSLLLTYLSMQNRG
                     LIIDDLTGVLNRQSFIQELDLNINTEQSGFIITIALDDFKFMNETFGTKNGDIALKEV
                     GKYLIQIHDVNHVYRIGGDIFSIVVNSQLGIEPDDIIRKIEERFKKPWLVEEISFNLA
                     TSIAVVYYPENAETTEDVIVAIDFSIHEAKRSESRRVVYADASISEKIKKKHVIKNCL
                     KEALKNDGFEVYYQPIFSNSRGRFTSAEALLRLEHKELGFIPPDEFIPIAEQTGLINS
                     IGLVVFEKVCRFIASDEFEDLKLDNIAVNLSVVQCMQKNLADDLLYLMNKYNVSPTKF
                     KLEITETVAMGSFNIIKETMERLIDLGVKFALDDFGIAYSGVTNMLSLPFSVIKLDKS
                     LIWSMNEDSRHKLTVETIIALINKLNMKAVAEGVETIKCAEDLIAMECEYLQGYYFSK
                     PVPEDVFKNLLKENTFTS"
     gene            37658..38068
                     /locus_tag="PC414_000978"
     CDS             37658..38068
                     /locus_tag="PC414_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDF2463387.1"
                     /translation="MNKKKILVPIDGTERSMHSLEFIKGIFKKDEVEVEIMNVKELVF
                     IDGISLAEEIKNSENLGRRILDKAAEIMGEYDVKVHFTFGYPGDEIIRKAKEDNVDFI
                     VMTKSTKKGLTRMIGSVTASVVKQAKCIVMIVPE"
     gene            38090..38392
                     /locus_tag="PC414_000979"
     CDS             38090..38392
                     /locus_tag="PC414_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIBC"
                     /protein_id="HDF2463388.1"
                     /translation="MNKKIVAVCESERSLNSLKTAANDLGFDLVYEIQENDNIKNELS
                     IYDIEDASIVLFVTSDSIESIEKIDRFIDREFYEVDPKYIIEDAKSIVSEILIDLN"
     gene            complement(38609..39301)
                     /locus_tag="PC414_000980"
     CDS             complement(38609..39301)
                     /locus_tag="PC414_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDF2463389.1"
                     /translation="MKSLNATEKIIDYIKLNILNGTFKINSKLPSERKIADLFNISRI
                     PVRNAINILCKEGILRAVPYSSPIVEGFKKVDLFDDGEIYKNHNIQEFYVESLRARQL
                     IESEATKLAILNATSKELQKIKYTYLKSIEELDKVSHGLIEECYDADLQFHKEIILAS
                     HNPIFIKYYELIPKTVSSNQYFGFKYRNSLKDMISHHNKIMIAFDSKDSYLGYTSMYN
                     HLEDVIQLFQHD"
     gene            complement(39327..40514)
                     /locus_tag="PC414_000981"
     CDS             complement(39327..40514)
                     /locus_tag="PC414_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2463390.1"
                     /translation="MKKISNQKLLLLSIVTFFWFSQYVYIPFQNPYLSACNVSTNMIG
                     IIIGAYGISQCLLRLPVGVFADSINKHKIFILSGIFFAGLASLIRVFSPGGNGFLIAN
                     ILSGISSSTWISYMVHYTNFFSTEHQQKATSTIILVNNLGMLIGFVMSTLFYEKLGMT
                     ALCIMSVLSSVIGTILALNIKNHNSKKIPEKISIKKYLSICKDKRLVFFSFMALIQQG
                     IQMSTTMSFTTQILKNLGSSSTLIGLSSIFYMVCAVASSGFASTKFCTKYGPKFWIPL
                     VFILVSAYCFLVPTIKNINIIFVLQVLPGISTGILLSYLTSESMKNVPKHQKSTAMGF
                     FQAIYAIGMSVFPMIVGKISEYTSIQYGYFILAIIAILASILSYTYYKKVDLTLNYDI
                     IQS"
     gene            40850..42727
                     /locus_tag="PC414_000982"
     CDS             40850..42727
                     /locus_tag="PC414_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2463391.1"
                     /translation="MDYKNIAQEILLNVGGKENVNEVTHCMTRLRFKVKSASKVNKDK
                     LSKTEGVITVVESMGQIQVVIGNKVKKVYDEVIKIVPQSNNIGKKNESQEKQGIINSI
                     LSAVAGIFTPTIPAIAGVGMIKGILSVLAMYYMNKNGVDIKETQSYIILNAMADSIFY
                     FMPIILGYTAAKVFNANKIISMVLGATLCYPTFTALMAGEESVRFLGLAVTKATYTSS
                     VIPIIIAIWALSYVEKVLEKYIPEVIKIIMVPTLSLVVMLPATLFLFGPIGIYIGNVI
                     NFSYKYIYELSPALCGAFVGGLWCVLVIFGAHRALLPIGISDVAQTGRQNLLAFAGAA
                     NFSQAGAALGVFFKTKNQGLKTISMSATITALFGITEPAIYGANLRLKKPMVCAVICG
                     AFGGAIMGMGGAYGNAFANQGVLTIPVYAEAGALGFLSYLGGCAIAFFGSAISTYLVG
                     FEDLEESSNKENSSIKVETKDGVIDITSPVEGECIELSEVKDDVFASKAMGEGIAVLP
                     TKGVITAPTDCEVASLFPTLHAIGLKLDNGAEMLIHVGINTVELNGKYFTKHVNQGDL
                     VKKGDKLISFDIDKIKKAGYDVTTPVIVNNTFDFGQVVSCKSSYVSTNDNIISLVLAG
                     N"
     gene            43084..44490
                     /locus_tag="PC414_000983"
     CDS             43084..44490
                     /locus_tag="PC414_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDF2463392.1"
                     /translation="MNTGFPKDFLWGASSSAFQVEGAWDKDNKGKTVADYNSFKKSHL
                     QADTKVASDFYHNYEEDIELMKELGMKTYRFSISWARIIPDGEGEINQKGLEFYNKII
                     DKLIECDIEPFVTLYHFDLPFKLVEKYNGWESRETVYAFERFAKICFKHFGDRVKYWQ
                     PHNEQNLIVRVEERINIYDETDSWKIDKMRAQMDYNLCLAHALAVNACHEMIKESKIG
                     AAVSSSVTYPLTSKPEDVYAARMNDNFKVYYMLDMHHYGEYPGYYMKYLEKRNIVPHM
                     EDGDKEILKKAKMDFIAVNYYRTNCAEALPEDSQHPFGLREGTVDFSMYGLFKMSMNP
                     NLEASEYGAAIDPSGLRVALNEYWQRYHLPVIITENGLGAKDILEDGKIHDDYRIDYL
                     RSHINACKLAIEDGVEMIGYCPWSFTDLLSSSQGFNKRYGLVYINRTDHEVLDLKRIK
                     KDSFYWYKEVIENNGIVK"
     gene            44518..45366
                     /locus_tag="PC414_000984"
     CDS             44518..45366
                     /locus_tag="PC414_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2463393.1"
                     /translation="MYITKVLNNSLLLAKDDNDEEIILMGKGIGHNYKAGSELNKKDI
                     EKIFVLHDENIKKSIIQLATEIDAEYFGIAQMIIAYGIEKYNLKLMNHIYLALTDHIA
                     FAVRRFKEGIMIENHYLFEIKEFNPKEYDIGKYAITVFKEVLGLDLPEEEIGNIAGHF
                     INAQQDNPYSDRNKRSAKIVNAILQIVHYHFSIVYNKESFYYRRFVMHLKAFSQRFLA
                     NEPSREKIDFIYEQVQKNCKEEYECVKKVGAYMYKEYSRELPRQEELYLMIHIHKILG
                     ELCENS"
     gene            46027..46719
                     /locus_tag="PC414_000985"
     CDS             46027..46719
                     /locus_tag="PC414_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003020373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2463394.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEQIEANYCHSGRDGLVELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKSDNYSKVHGLRAGADDYLTKP
                     FEITEFTARVLSLIRRYTRFNPKNEEPQQLSFKGLLIDLDQHTVTTNGEPMLLPPKEF
                     GVLLYCARHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            46716..47651
                     /locus_tag="PC414_000986"
     CDS             46716..47651
                     /locus_tag="PC414_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2463395.1"
                     /translation="MKMDEQTIILFLLLLSIGLLFLSIFAFSKLGHIYKRLKDIEEIL
                     ADVQIGDENRKILIKPCDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYLDAVHSGIVIEQEREEYLEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLNFEIDIPENRLMVNLDPDGYSRVVNNLVQN
                     VLAHSKARQIKITMSEDSRMVLLRVEDDGVGIARENLPHIFERLYKCDKGRSEKGSGL
                     GLSIVSQMVERMGGQVSVESEIGKYTVFTVSLPLI"
     gene            47799..48722
                     /locus_tag="PC414_000987"
     CDS             47799..48722
                     /locus_tag="PC414_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2463396.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGAGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALDVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRTFIRDLCTERGKTILISSHILSEIALLADDIGIIDHGILLEEESL
                     AQLEEKNSKYIHFAVSDAAQAARLLETQLNLKNFKVADNYNIMLYDTDVSVAEINRAF
                     ILNNIEVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            48706..49476
                     /locus_tag="PC414_000988"
     CDS             48706..49476
                     /locus_tag="PC414_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2463397.1"
                     /translation="MKELLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVQKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVAVKLGMSIAFGIMFFVTVLPVVVLIVYF
                     NRSYIFSIILSFVYAIFNFSISLNIINFEPNNPILSVLPAPVIMRWWMAFWGDPTGEY
                     TALRQPYLLSTPACVGILCLITIIAVLLICMIYKKQEN"
     gene            49486..50268
                     /locus_tag="PC414_000989"
     CDS             49486..50268
                     /locus_tag="PC414_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2463398.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQVVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFGITLIGGYLINREYTDQTLKNILTVPISLRKLLVGKL
                     IAVGGITVLLSLFSFLCTFILGLLFCNINISVTLVVKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNAFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLVGYNNGAGTVYQPLI
                     GAMTLIVILILTTILLVFTPNYDKVMTISKKKGIKHKKYSHK"
BASE COUNT        18647 a         6139 c         9235 g        16522 t
ORIGIN      
        1 taactaaaag tatataaaaa tgatgaataa atatatatta tacattatca atgataggtt
       61 atataataaa atcactaaca attatattat aagaaaatta taaaaattac aaggaggtat
      121 agaaatgaaa gaaaaagcag ggattagaaa tgccataata gcagccttta taatcacaat
      181 attggcagaa ttaattggac ctatttcatt taaagttatg ggaattaatg taactttact
      241 tagtatacta tgggctatat tcataggaat ggctgtttct cctcatttat tagggagagt
      301 tataccagcc ttaaaaaagt ttatagggga taatgaaata aatgtatctc catatttatt
      361 atctttgaca ctatatccat tagggataat gtttggaatt aatgcagggc ctaaagttgg
      421 tatattactt caagcaggac cagcactttt attacaagaa tttggtcata tgggtacaat
      481 gttgatagca ttacctgttg gagtagcatt aggtcttgga agaagtgcat taggtggtac
      541 tttttcatta tgtcgtgaca cagcactagg aattattgga gataagtatg gattaaactc
      601 accagaaggt ataggaacac taggaactta cataagtggt agtatatttg gaacattatt
      661 attttcattt ttagcaccag tgggtgtact tttaggattt catccatatg ctttagcaat
      721 ggcatcagga atgggtagtg gttcaatgat gggagctgca acagcatcat taatgaatac
      781 agttccacaa atgtcagagc agattttagc atattcagct actagtggat tgattacagc
      841 tgttactgga atatatatag aattattctt atctttacca gtagccaact actattatag
      901 caaagtagct ccagttattg aaagatttag aggaagaaaa acaagaagaa caagtaataa
      961 attgtaaagg aggatatata tatggaaaaa acttttaaga ctattatgga gcagataaaa
     1021 atagtattgt ttatatatat tattatttgc tttggacaaa tgatagcact taagactcag
     1081 gctcaagatt tgttgattgg aagtgtatct ggatttttgt tggtgataat tgctattata
     1141 ataaagaatt ttgttaaaaa acctaattta ccaggttttg catgggctac attagttgca
     1201 tttgttttat cgcttccaat atctccagtg ggggatatca tagcaacaaa tgtagggaaa
     1261 atcaatttta tgtctacagt aacaccatta ttagcttttg caggaatctc tgtaggtaat
     1321 caattagata tattaaagac tttgagttgg aagttagtag ttatatcatt tatagttatg
     1381 acatctactt actttggttc tgcttttata tctcaaacag ttttaagatt aaatggaatg
     1441 ctttaaggag gtagtaaata tgaatgaatt aaaagcaaaa attaaagagg aaataaagtt
     1501 attatctgaa gagaaaaaga catcatttga aaaagtttct gactatttat tttcaaatcc
     1561 agaattagca tttgaggagt ataagtcaca aaaagcatta tgtgatttgt tagaagaaaa
     1621 tgaatttaat gtaactaaag gtgtaggagg attagaaaca tcttttgaag ctgtgtattc
     1681 aaatggaact aatgggaaaa cagttgcttt tttagctgaa tatgatgcac ttccaggaat
     1741 gggacatgca tgtgggcaca atatcatagg gacaagttct gttggtgcag gtatcatatt
     1801 aaaagaaatc atgaaaaaac ataatataga agggactgta aaggtttttg gaacacctgc
     1861 agaagaaaga gtaggtggaa aaataactat gattaaagaa ggtgtattta ataatgtaga
     1921 tgctgcatta attttacatc caagtgatgc ttcaatgcct gatgatatat catttgctca
     1981 agttaattta aaatttgatt ttactggtaa ggcatcacat gctgctgctt ttccttggga
     2041 agggaaaagt gccttaagtg gagtaatagc actatttaac agtgtaaata gcatgagatt
     2101 acatcttaaa gattatgcaa gagtacatgg aattataact gatggaggta gcatacataa
     2161 tataattcct gaaaaatcta cagctatatt taatgtaaga gccttaagta tagaatattt
     2221 aaatgagatt tgtgaaatgc ttaaaaattg tgctaaagga gctgcaattt ctactggaac
     2281 taatgtagaa atagttcaat tagatgaaat atacaaagag attaagaatg attctgaatt
     2341 agtgaatata gttagacaaa attttgaagt tttgggtgaa gactatgttg aaagagattt
     2401 aagtcaaggt ataggttcaa ctgatacagg aaatttaact catgagatac ctgcaataca
     2461 agcatatata aaattaaaag aaaatactgc aactcataca gatgaatttg ctgttgcagc
     2521 tggtggtgaa gaaggcagag tagcacttat aaaagcaatt aaggtgttag caatgtgtgg
     2581 agtagatatt ttgtatagta aataatatat aattttaaaa tatattaaga aaggaaatcg
     2641 gattagacat gatattaaaa caagtaatag aggtttatga cattttagat aaagcaaatg
     2701 ctaatgggga agaagtaaaa acttacttac aaggttatgg tgaagtagat gtaactgtaa
     2761 aagaattgtc ttcaagtaaa ggttcaacag atttagtaaa gcttacaatt cctggaaaaa
     2821 atggaaaatt aaaaggtgga gatgctccta ctcttggtat tttaggtaga ctaggaggga
     2881 ttggagcaag accagaagtt ataggttttg tctcagatgg tgatggagca ttggtagcaa
     2941 ttgcagtggc tgctaaactt ttggatatgc aacgaaaagg ggatatatta gatggtgatg
     3001 ttgtaatttc aactcatatt tgcccagatg ctccaactaa agaacactat cctactcctt
     3061 ttatggattc tccaattgat atgatgacta tgaatgaaaa tgaagttgat agtagttgtg
     3121 atgcaatact ttctatagat actactaaag gaaatagagt aatcaatact agaggatttg
     3181 caatatcgcc aactgttaaa gaaggctaca tattaaaaac tagtgataac cttttagata
     3241 ttatgcagac tgtaacaggg aaatctccat ttgtattccc tttgtctatt caagatataa
     3301 caccatatgg aaataatctg tatcacttaa atagtatttt acaaccagca gtagctacag
     3361 atgcaccaat agtaggagta gcaattacaa cagagatgcc tgttgctgga tgtgctacag
     3421 gtgcaactaa ttttagtgat ttagattcag ctgggagatt tgcaattgaa gttgccaagc
     3481 tatttggtag aaataagtgt gatttctatg ataaagaaga gtgggaaatg cttattaaga
     3541 gatatggcaa attaaataga tttcaaacat ttggtataca gtaattagaa aaatattaag
     3601 tttatataat ttgtgatata actatttaag aatatataaa caattaggaa taaaagtaag
     3661 aataaagcac ttagttaaaa actttaaaaa taaaatattt atttgtttat ataaaaagga
     3721 ggattctggt tattgggatt ctctttttta tgaatacaaa taatatattg taattataaa
     3781 aaaaggagcg tttcaaagtg atttttttaa tcaccttgag acaactcctt atatttttat
     3841 ttaaattcta ttttttatta agttgctgga gttccaccac catcaggagg agtagtagag
     3901 ccaccaccat tatttccacc accatcagga ggagtagtag agccaccacc attgtttccg
     3961 ccattatcag gaggagtagt agagccacca ccattatttc cgccattatc aggaggagta
     4021 gtagagccac caccattgtt tccgccatta tcaggaggtg tagaaccgcc accattatca
     4081 cctgtgttat tattgttatt attattttct tctggttggt tattgtctgg tttttttgta
     4141 tcttcagatg atggttgaga acttacttta gtaggagcag taccttcaat aaatgctgct
     4201 ttagagccag aagattgctt tcctccatcc tctaaattaa tttttgtaaa atatacattt
     4261 ttaggaactt caaattcagt aaccgttaaa ttagcatgta ttttttccat aattttttcc
     4321 catagttttg cagtactagt acttccatgt ggtacttctc tacgttttat agtgttacca
     4381 gaatcatctt ttctaccagc atcatctcca atataagttg caccaacata ataaggagtg
     4441 taaccgacaa accaagcatc atatgcacta tttgtagtac ctgttttacc tgctacaggc
     4501 attccctttg gaagtgtagc aacaccacca gtaccttcag taattacgct ttctaacata
     4561 tctgtaacaa cataagctac ttcagggtct acaactttat gttcttcagg agcattttta
     4621 actaataatt gaccatcaaa ggtagttata gtagtaaaaa tagaaggttc tacataaata
     4681 ccgtgatttg ccaatgtacc ataagcagca gccatttgaa gtggagtaat accattcgcc
     4741 ataccaccta gtgtcaatga tgggaatttt ctatcacttg cttcagatga acctgagtga
     4801 ttattcttta aagttgttat accaaagttc tttaagtaat ccatcattat atctacacat
     4861 tcatcataag agtctccaag catttcagca gttttgacag ctatcgtgtt tgaagattta
     4921 gcaagtgctt tacgtaaact catagaacca tgaccagcag tagtagttct tggattccat
     4981 ttattatttt cttcaaactt atatcctcct cgagaatcac ttagagcagt tgcttgtgtt
     5041 atttttagag tatcaatagc tggcgtatat attgctaaag gttttataga agaacctggc
     5101 tgatgagggt tagtagcacg atttaaagtt tttctaccac ttatatttct tcctccagct
     5161 agtgctctta tttcaccatt tttataatct aagattacca ttgcagcttg cggttggcta
     5221 actccatcag gaccgaccat atgtcctggg aaattgctat ttctatcaaa ttcttcttct
     5281 aaagcatctt gcattttagg gtctatagtt gtgtttacaa ttagaccacc attgtagaat
     5341 aagttttgag cttcatcatt tgtatgtcct tgttccatta aagcatttat aacttcactt
     5401 tcgataaggt cttctacaga tgaagaaact ttatctgatg attgaggcaa ttttatttct
     5461 attttagctt tgattgcttc atcatattca ctttgtttta tatatttcaa ttctaacatt
     5521 ttctttaaaa caatttcttg tcttttttta gcttcaggat tacttacagc ctttctaaca
     5581 actaatgtac ctgcttttaa ttgtctataa gtatcattag atataagctc ccaagatttt
     5641 attttttcaa tcatatcaaa gtccacttga gtagggtcat ctaaatcatc atcagttgtg
     5701 ttaacaaaaa ataataattt attctctaaa tcctccttag tttcatttcc ctctaactta
     5761 gaagttttat aggctgaaaa tcttgatgga tttttagttg aaccagcaag aagtgctgac
     5821 tctgctagag taaggtcagc tgcagactta tcaaaataac cacgtgcacc agcctcagca
     5881 cctgctagac ctctaccaac gaagaagtta tttaaataag tttctagtat tttatcttta
     5941 cttactgttt tactcatctc atgtgcatag taaatatcct ttattttacg aggtatagtt
     6001 tgctgttcag ttgttaacag cattttggat acctgcatag gaatagtact accaccttgc
     6061 ttagtacctg taagggtctt taatactgac ctaaataaac ctttaatatc gacaccctta
     6121 tgctcataaa aacgctcatc ttctattgca attacagcat gttgaagata aggggaaatc
     6181 ttatctaatg gaacgggtgt ctttttattt acacttggag ctgtagataa tgtttttcca
     6241 ttagcgtatt taatagtagt tgtttggttc gtctgtttat ctaaaacagc ttctgtaact
     6301 ggagttacat ctcttagtga cgcaaatact agaccagcag tacctgctgt accaacaact
     6361 agtaaaacta aaaatactat cccaaatact cttagttttt taaatttatc gcttttttta
     6421 gattttttct tgttttttgt tgaacgagtc ttgttagctg aagttctatt aattggttta
     6481 tttgtattac cagatgagct aacttttttt ctcctgattt tatttccatt tttatcatta
     6541 tcgttattca taagatttta acctccttac ttaatgcaat attataacac aaataggagt
     6601 agttttgtaa ttaatagaaa aaatgaattt gctataatat tctgtacatt gtatatgtat
     6661 aaacgtaata tattaaaaag tctatatttt tattttattt atttttgaca aatattataa
     6721 aaattatgcc aaagctacat ataaataaat aggaggggat aattttgaat agaatagtag
     6781 atagaaaaac aaaaaatgaa tttagaaaaa tcatagctat ttttttagta attcttttct
     6841 tgtctgtatt tataggcagt tttatatata tagataaaac tttaagaccg acaataacag
     6901 ttttagcaga gactaaagct cttgaactag caaataggtc gataaataaa gctgttgctg
     6961 agatggtaga aggaaaaata aattatgaag atttaatgga tatacaactt gataataatg
     7021 gtaaaataac tatgatacaa gcaaacacta taatgatgaa tgaaattgca tctgcaatag
     7081 ctttagaaat acaagatgaa ttaaaaaagg ataagacagc aagttcttac atacctatag
     7141 gaacagcatt aggaagtcct atattagcta aatacggtcc taaacttgaa gtatctattg
     7201 aacctatagg aacagtatca gtaaacttta aaacggaatt tgagtcttca ggtataaatc
     7261 aaactagaca tagaatatat ttagaagctc aaacacaagt taaagtagta ataccactta
     7321 taacttcaac aaaacaaatt aaagctcaaa tacctatttg tgaaactata atagtaggtg
     7381 atgttccaga aagttatgta aatattccag aaaaaaatct aggaaatgtt ttacctaaca
     7441 ctggaaagaa taccaacaag taatgtaaag ttgtatattt ttaaataaat tcaaaataat
     7501 agtttataaa taaaattttt ggaaataata ttaatataat tttaataaaa atttatattt
     7561 tatactatgt aaaatatcta atatatttgg tctgtaggtt aaataaaagt acctttagac
     7621 caaatatatt attaataact taataacatc ttaaaactta tttatttata ttatttacaa
     7681 catccattta ataaatacac cataacctac agctgggtcg ttttcaattg catgtgacac
     7741 tgctccgatt attttattat cttgaactat tggagtacca ctcatacctt gtacaatacc
     7801 accagtttga gctaaaagct ttttatctgt aattttaatt ttaaaacttt tactttctgg
     7861 atgtttttgg ttttctatat ctataatttc aatattatat tttttacact tattaaagtt
     7921 atcttgtaaa attatttgag cttttcctaa tttaacttca tctaaagatg ctactttata
     7981 tttttttaac ttggaaatat caaagttatt aatttgacct cttattccaa aatcagtatt
     8041 ttctgtaaag ctaccaataa tattttctcg ttttgcattt atacatccaa cactaccttt
     8101 ataagatttt tctatattaa ggtgcttagt tgtagaaatt gaacctgttt taataggtat
     8161 ttttcttgca ttaccaacac ttattggatg acctacagct ccaaactctg atgtatctgg
     8221 attgatatac gttaaagtag caaatccaga aagtaaattg ttaaaactaa ttttttctaa
     8281 tatgtgtttg gtagttttca ttgttataag ttgtccacct ctatttaata aaatagatac
     8341 ttgtttttct tctggtaaag catttaaggt attggcaaag tcactatatc catcaacttc
     8401 agcattattt acctttacta tagcatcacc caatctaaat ggagatgctt ctccaggatt
     8461 tctaactatg atatttttta attctgcgtc aatttgaagt acgtttccaa ttggtattaa
     8521 atattcaggt tcattttttt gtaaattttg tgcataaatt aaattatttg agaaaaaata
     8581 taataaaaat aaaaatgtta aaacaattgt aaattttgac ttagctaagt tggttttttt
     8641 acttaaagta ttatttttaa aatgaaaatt tttaagttgc ataaaacacc tctttcttat
     8701 taaattattt taataataga attgaagttt tcttgctgct tctatattat gttgtgcaag
     8761 aatcatgttt tgatgcttgg aaaactagct caataacacc ttagttttca taaaaataaa
     8821 taaaataaat agtgtatatg ataaaataaa tacatattca tataaataaa aataaacaat
     8881 aactgtctag cataagaaag gagcaaaaaa tgaaaaaata tagattgtta gttacttgta
     8941 taattgccat gggaatcata attataggaa catcaaatcc agttagcaat ttttacaaga
     9001 ctacaaattt gattagttca ggaaatatca caaatgaaaa agcagataaa gatgaaaatt
     9061 caaacaaaaa taataaagat aaaaatgaag acaaatctaa ttctaaaagt aataaagata
     9121 agtcatcaga ggacaaatcg tcagataata aaaatgaaat tcaaaaaaat aaaaaacttt
     9181 taatttgtat agacccaggg catcaaggaa aaggagatag caatttagaa ccagtagctc
     9241 caggttcttc ctctaaaaaa gcgagagtat cttcaggaac agagggtatt gctacaaaaa
     9301 agccagaata tgttttaaac ttagaagcat ctcttgtttt aaagagtata ttagaatcta
     9361 aaggttataa tgtaataatg actagagaga cacatgatgt aaatataagt aattcagaaa
     9421 gagcaatact tgctaatgac aaaaaagcag atatggttgt aagaatacat gcagatagtt
     9481 taaataattc ttcaaaaact ggagcatcta tattgattcc tgaaaaagat ggaaaatata
     9541 cagctcctat atatgaagaa agtaataaat gtgctgaatt tatcaaacag aatatggaac
     9601 aatcaggtat acaaatcaat ggaatcgttc aaagaggtga tttgactgga ttcaactggt
     9661 caaaagtacc agctgtatta gtagaaatgg gatttatgag taactataat gaagaccaaa
     9721 tgatgtcaaa tccagactac caaagaaaaa tgatgcagtg tatagctgat ggattggatg
     9781 cgtattttaa ataagatatg aatagagaaa aagttaggtt ggtattattt gcctaatttt
     9841 tatctctatt ttttattgta cataacacaa agaagaagtt tattttaggt taaattgtcg
     9901 taaatataac taaaaatatt gggaatactt gatttaagtt taaaaagaat atattatagt
     9961 atagaatgtg taaaattttt ttgagaggtg ttgaaatgct agtagtagaa aatgtgagtc
    10021 atggatttgg tgcgagaaca atattagaaa atgtatcctt tagacttaga aaaggtgaac
    10081 atattgcact agttggtgct aatggagaag gaaaatcgag ttttttaaat ataataacaa
    10141 agaagcttat gccagatgct ggaaatataa aatggtcttc aagagctaca gtaggttact
    10201 tagaccaaca tacggtatta agcaagggaa aaactataag agaagtgctt agagaggctt
    10261 ttaagcatat gtttgactta gagcaagaaa tgattgctat gtatgacaaa atgggagaag
    10321 ctagtgatga tgaaatgagt aagcttcttg aagaaacagc agagatacaa actatacttg
    10381 aaaatagtgg tttttatatg atagatgcaa agatacaaga agttgcaaat ggtcttggtc
    10441 ttggagaaat tggtcttgat aaagatgtaa ctgacttaag tggagggcaa agaacaaaag
    10501 tacttcttac taaacttcta cttgaaaatc caactattct tatattggat gagcctacaa
    10561 actatttaga cgaagagcat ataacatggc ttactaagta ccttcaagag tatgagaata
    10621 gttttgtctt agtttctcat gatattgaat ttataaataa tacatgtaat gtaatatatc
    10681 atatggaaaa tggagagtta aatagatata aaggaaatta tgatgaattt gtaagactta
    10741 atgatataaa gaaacgtcaa gaagaacaag cttatgataa acaagttgaa gaaagaaaaa
    10801 gactagagga ttttgtagct agaaataaag ctagagtagc aactagagga atggctaaca
    10861 gtaggcaaaa gcaacttgat aagatggaaa tattagaaag accaaaagaa aaaataaaac
    10921 ctacttttgc ttttaaagat gctagagcag ctagtaaaat aatttttgaa acagaaaatt
    10981 tagttttagg gtatgatgaa gcattgacaa aaccacttaa tttccattta gaaagaggaa
    11041 aaaagattgc cttaaaagga atgaatggta ttggaaaatc taccctatta aaaactttac
    11101 ttggaataat aaaaccattt gaaggaaatg ttaaattagg tgactatttg gaagttggat
    11161 attttgaaca agagagttca agagaaaata gtaatacacc tatggatgaa gtttggagtg
    11221 aatttccagg gcttacaaac tttgaagtta gacaggctct tgcaaaatgt ggacttacaa
    11281 atgaacatat aacgagtcaa atgagagtat taagtggtgg agaagctgct aaggttagac
    11341 tttgcaaagt aatgttaaaa aatataaatt tcttagtatt agatgagcct acaaatcatt
    11401 tagatgttga ggcaaaagat gaattgaaga aggctatcaa agagtttaag ggaacagttc
    11461 ttttagtatg tcatgagcca gaattttatt cagaaattgt tgatgatgta tggaatatag
    11521 aagattttac aactaaaatc gtgtagtgat ttaacttatt gctgggggga tttcatgaaa
    11581 aagtttctaa atgtactttt ttcaatttgt attagtattg ttataatagt aggtgttatt
    11641 aattttactg ttggatttaa acaactttat tattttgaca ttgattactt aaatatatca
    11701 gagttatctg gtttatctaa agatgatata aaattgaact acgattatct aattgattat
    11761 aacttaaata agaatgtgtc tgagttcaaa ttaccaacat taaaatcttc tccacaaggg
    11821 aagatacatt ttgaagaagt caggaatata tttcaaaata taaataaatt agctaaatta
    11881 ttattggtag tttctttagt aggaattata ttgagtgtaa aaaataaaaa tataaaaatc
    11941 ttaaaaacta catcaataac acttataatt atgccattat tattaacagt tccaatttta
    12001 ttaaattttg aaaagtcatt tattattttt cataaattat tatttagaaa tgattattgg
    12061 atatttaatc ccgatttaga cccagttata aatatattgc cagaggaatt tttttttcac
    12121 tctggaatga tgattttaat attaattttg ttagtaagta tagtactgtt tgtaatgtat
    12181 aaattatgta aatctttatc aataaaaata tttcaatgtt aaatagaggg atatttttat
    12241 ttccaaaata agataataaa aaaggattag attaaaagtt atacaataaa aatagctgtg
    12301 caataatatt tagactatga aagtagttga aaaatattta tgcacagcta tttataatag
    12361 aatttacttt ttactagtga ttttaactaa atcttgaaat ttactcataa tgtaattgta
    12421 attatctttt ttatgaggta atatacaact actgcaatct ttgatacctt tatctgtata
    12481 cctaaagttt ccaccacagt tttctcctaa agcatacaaa ggacaatagc aaaatagaca
    12541 gttaaactct tctggtttat tagttttatg acaagggaaa aattcgcaat ctttgtgatt
    12601 aaaaaactta taattttcgc ccatattttc ctcctaatta aataaaattt aaatttttta
    12661 tactatataa gattttggtg ataatataat tatactaaat aattttatga atagtcgata
    12721 atatagtatt attaatcata gtattaatgg tataattagt caacagaaaa ataatgtaaa
    12781 aataatgtaa gtgtattttg taattagaat atgttttata acaaaaaatg aggtgattaa
    12841 gtgaaagaaa ttcaagaaaa agcacttctt gtaggtctga atttgactac aatagtgaaa
    12901 aaaaatgatg atatagacac taatgaatct atggaagaat taaaagaact aacaaaagct
    12961 gctggggcag aagttgtagg tagcttaatt caaaataaac actctgtaga tgcagcttat
    13021 tatataggaa aaggtaaagt agaagaaata agagcctata gtgactcttt agatgctact
    13081 ttagtaattt ttaatgatga attatctggt gctcaaatca gaaatataga gaatgttgtt
    13141 ggaagaaaag taatagatag aactacttta attttagata tatttgctca aagggcactt
    13201 agcaaagaag gtaaattaca agttgaatta gcacaattaa aatatagatt gccacgactt
    13261 tatggaatgg gtggtgagat gagtagaaca ggtgcaggga ttggtactag aggtcctgga
    13321 gaacaaaaac tagaaataga taagagacat atacttaata aagcagcaga tataagacgt
    13381 gaacttaaag aagtaaaaaa gaatagagag acacagagag ttaagcgttt aaagtcaaat
    13441 ataccgattg tagcacttgt tgggtataca aatgcaggaa aatctaccct gttgaacgaa
    13501 ttaataaaga cacacaagga ttatgaacaa gaaaaagaag tttttgtaaa agatatgctt
    13561 tttgcaactt tagatgtaac tttgagaaag gctcttttgc cgaataaaaa agaattttta
    13621 gtagtagata ctgttggatt tgtaagtaaa cttccccatg atttagtaga agcgtttaag
    13681 gcaacattgg aagaagtaca gtatgcaaat ttaatcttac atgtaattga tgcaacaaat
    13741 actagttatg aattgcaaaa aagtacgact gaaggtgtat taaaagaact tggtgtaaat
    13801 gataaaaaac atatacttgt atataataaa gtggacaagc tagaattgga tatataccct
    13861 aaaagtcaag aagatattgt gtatatttct gcaaaacaag gcataaatat ggataagtta
    13921 ttaaatatga tagaaattgc tttaatggaa aatacttatt cagtaagttt gatgttacct
    13981 tatgagagag gtgatatatt tagtagaata aaagataaat ataatgtgga gaattttgag
    14041 tatggggaaa gtggtataac gctagatgca gatttagatg aagaagattt taatatctat
    14101 agagagtaca tactagaaaa ataggttgaa tatggatata aactgggaaa atatagataa
    14161 tcttgaagat tattttataa cctatcttct ttataaagaa tctaaaacag tatctcaaat
    14221 tagtaaaatt agaaatattt caagtactga agcaagtgag caacttatac aagcaaagtt
    14281 aaaaataaag gaaatgcaaa aagatgattt tgaggcatct aaggatattt tggataaatt
    14341 tctagaacta gataaaatta agagacttga ttttatggat agcttagatg acgaaaaaat
    14401 ggtatacttt aagagaaagg ttttcaaaag aatccttgta gaaaaaaatg ctgaagattt
    14461 aattgtttta atttgggcga caggtgagct aaaagatgat aggtttttga aattgcttca
    14521 tcagctcaca aaccacagac actcagatat tagaagaata acttattcag caatcagaaa
    14581 gattgaatca ccaagtagta gagaagtact gcaaaaaggt ctctacgaca aaaatgcaca
    14641 gactaggcag tactgtgcaa aagctctttc aaaattggga gatgaaaaca gtttaaagat
    14701 tttacagcag ttgaaagaca agaataagaa ttttgaaaaa gaatatgtgc ttagagctta
    14761 tgacgaagcc attaagtcat tggaaaatgc aaaaaatttg aaaaagtaat tttttattaa
    14821 agcaatttgt ttacttaatg atgtatttct aaagtttaac aaggtaataa ataaattaat
    14881 gggaggggtt tcaaatgata gtaagacgtt gtgcaggtgg agtagtgttt tatgctaata
    14941 aagtacttat tgtaaaaaat gataggggtg aatggacact tccaaaagga aagatactag
    15001 gagggggact tccatatgag agtgctgttc aaagagttaa agtagaaact ggcatagatg
    15061 ctaaaatgat agatgtggct ggcgatacta tgtatgaatt tttctcaaga agtagacaac
    15121 aagaagtttg taatgcaata atgtggtatg ttatggaagc ttgtaacact gattgtgtac
    15181 ttgctcctga atttcaagaa ggtggatttt ataaagtaaa ggatgcttta gaaatgttat
    15241 cacatcataa agagcaagct ttagtagaag tatcttataa aaagtttaag gaattaaaga
    15301 aattagtttc agagggcgat gatgttccag caaattaatt gtgaatttag aggattctga
    15361 ggaagggata gccccttcct tttttaattg aagcagtttt aagcgtaaat tatatagtct
    15421 ctttaaacta tgatttatca atttagagat ttagtagttt atatttaaaa tcagtaagat
    15481 atatttaaaa taaaaattaa aaagtataca attttattat ataattgtct tatgatgttg
    15541 attaaagaag tattaaaagt ccatttattt ttaaatagaa tgattttata aatatatatt
    15601 tgatgtgtat attagaaaat gattaaaata ggaggaaata caatgagcaa ttacaggaca
    15661 ttacatgaat ttggaacgga tgaaataacg attgaaaagt ctgtttttat tggatatgct
    15721 aagcctataa agagtgaaga agaagcatta gagtttataa atgaaataaa gaaaaagcat
    15781 aaagatgcaa ctcataatgt gtgggcttat actgtaggta aaaatatgaa tatacaaaga
    15841 tatagtgatg atggagaacc acaaggaaca gctgggatac ctacattaga ggttataaaa
    15901 aaagaagatt tgagagatgt agcggttgtt gttactaggt attttggagg aacaaaatta
    15961 ggtgcaggtg gtctagttag agcttataca aaaggtgcta aactgggact tgaagcaggt
    16021 aagataattt ataaggtaat gtatcaagaa gttaaggtta agatagatta tactcaactt
    16081 ggaaaagtgc aaaatgagtt aatgaatttg gggtatttta taaaagatac tgtatatgag
    16141 gataatgttg aaatagtagt atattcaaga ttagaagatg tagagaaatt atcagaaaaa
    16201 atgattgata taacaagtgg aacaggaaaa atagttttag gagaggaatt ttatttgtct
    16261 gaacaaaatg gagaaatttt aatataatct aaaaggttat tagaatagtt aatacctcaa
    16321 atagccaatt agtattttta agtgaaaagc cattaaattg atttaatcta aatcaattta
    16381 atggcttttt atcggatttt gcacagaatc ctttttttac ttcaaataaa ccgactaaaa
    16441 gtaaaaatat cccaaatatt ttttttaata tatctgaaga cagataatta gctaatagtg
    16501 aaccaaatac agctcctaaa accccaaaca cagctatagg tataaccaat ttaaaaatta
    16561 catttttatt ttttatatgt atagcaagtg ctataagtgt catagggata gatgaaagta
    16621 aatttacgct ttgtgctatt ttagtgtcta tgccagcaag taaaacgagt gcaggtataa
    16681 ggattgttcc tccacccatt cccattcctc caataattcc tgcaaaaaat ccaattagag
    16741 caaataacat taaaacacca tccttaatgc tgcaattatc ataattattc caaaagaaat
    16801 tctaagaaac tttccagtaa ccttatttag taatttagct cctataatac caccaagaat
    16861 acttccaact gcaactttaa ttgttaagtt aacatcataa gtacctttag atacatataa
    16921 aacagaactt gctgtcgtta agaatattat tatagaaaga gcagttgcat gagatttatg
    16981 ttcttctacc ttaacgatgt catttaatat tggaactaaa agagtacctc ctccagaacc
    17041 aaaaactcca tttataaatc ctgtaaaaac tccaattatc gtatttttta aattaaaatc
    17101 aaagtatttt ttctttaaat catcagtttt tttcataaaa atcaccttct gaaatagttt
    17161 taccaaaata ggcaaattat atttatataa aataagatta aatataatct ttcatgtttg
    17221 tttaattttg cataaaaaaa aacatattaa aacaacatat taatatatgg tataatatat
    17281 taaaacaaag tagcaaattt gagaggagat aacaatgtca aatataaaaa tacaaataga
    17341 caaaactgta gaatggctta taaataaggt taatgaggca aatgcaaaag gattaattgt
    17401 tggtgtatct ggaggaattg attcggcagt agttgctaat ttaattaaaa aggcatttcc
    17461 agaaaactct atgggagtta taatgagcat aaaaagtaat cctcaagata gagaagatgc
    17521 acttaaggtt atagagggat gtgatataga ataccttgat ttagacctta tagaaccaca
    17581 aagtgctata ttagacatgg tagtaggaaa tttaaaagat aaacatttat atagagaaga
    17641 atatttaaag atgacagatg caaacttaag agcaagagta agaatgagta caatttatac
    17701 aattgctaac aatctaggat atctggttgt tggtacagac aatgctgcag aaatacacac
    17761 aggatatttt actaaatttg gtgatggtgg agttgatatt ttacctattg caaatttaac
    17821 taaaggagag gtgtatgaat gggcaaaaga gcttggtgtt catgaagatt taataaacaa
    17881 agctccttca gcaggtcttt gggaaggtca aacagatgaa gatgaaatgg gaactactta
    17941 caatatgata gatgctgtat tagaaggtag attggatgaa gttcctaaaa gagaccaaga
    18001 gataattgaa agacttcata gattaagtga acacaagaga aaaacgcctg cacaacctcc
    18061 taagttctaa tttactattt ataagatttg tgataaaatt tacaagtgat gaaaattaga
    18121 ataattaatt gtacgtatta aggagtggaa atatgggacg tataggtaat ataattaata
    18181 gaaaaggtaa acaagatgct caaagagcaa agatatttac taaacatgct agagcaatag
    18241 cagttgcagc taaggaaggt ggagcagacc cagagtacaa tgcagcgtta aaaacagcta
    18301 tagaaaaagc taaggcagat aatatgccaa atgataatat agatagagct atcgctaaag
    18361 gtgctggagc aggtgctgga gaagactatg aaactatagt ttatgaagga tatggacctg
    18421 gtggagttgc tgttatagta gaaactttaa ctgacaacaa aaacagaact gctggtaacg
    18481 taagatacta ttttgacaaa aacggaggaa acttaggaac tagtggatgt gtgtctttca
    18541 tgtttgataa aaaaggacaa atattagttg gtttaggaga tggagtttca gaagaagaat
    18601 tgatggatgt tgcattagaa gcaggagctg aagatttcat aacagaagaa gatggttatg
    18661 aaataataac tacaccagaa gatttttcta gtgttcgtga tgagttaaaa gctaaaggat
    18721 atgagtttat atctgctgat gtgaagatga tacctcaaac tacaactgta ttgactgaag
    18781 aatctcattt aaaaatgatg aataaattag ttgatatgct tgaagaagat gatgatgttc
    18841 aagatatata tcataactgg gaagtagaat aaatagtata agcatattga aatttataag
    18901 tattgaatca attttaacat gtgataaatt ttatagaata agaaacacta aaatagacat
    18961 acgcacctct aacctatatt tgcataataa tatgtaagca taggtaaggg gtgtttttat
    19021 atttataggt taagaaattt aaagtaaatt ttgagctgga ctcgattgtc tataaataat
    19081 tgatgggtat ataacttgtt gctgaatacc atttatacgc ccagttactg tatcaataat
    19141 catcctaaga ctacgttctg ctgtctcttc catttttaaa tctataactg tcaaaggagg
    19201 aatacttctt gccattagtc gtgaaggtcc attacttgct gcaaatattt gaatatcctc
    19261 tccaattaca attcccgatt catgaaatgc attcatcata ccaagagcta ccacatcatg
    19321 attacaaaaa attacttttt ttagtgaatt ttttgcaagc attttttgag caatatcata
    19381 tccactatct attttattag aacaataata tatagaatct gatagatgta ttccatgttc
    19441 tttacatgtt tttaaaaaag atttacccct taaagtcata catgtaaagg aagttgggtt
    19501 gaatacaaca gcaatatcat ttttaccatt ttgaatagca tgtaaagctg ctatacgacc
    19561 agtttcctca ttatcaatag atacacttga aaaaccaggt atttcacgat ttacaacaac
    19621 tacaggaatt ttaggtttat tttttactag atattctata tcatcagcat tgatacctgt
    19681 taaaacaatt ccatcataat aattatttgc aaaaaagtat tcatcatcac atagatgacc
    19741 aactttgtaa ggtcgtatga tgatatttat gtttaaatgt tctaaagaaa ttatgtggtt
    19801 tattgcttga agtatattgg ttgatgccaa ctcaaatccc tcatcctgcc aataaaaagc
    19861 tattttaata ttattatgtt taataggagt agagcgttta tatccaagct tatcagctga
    19921 atttaatata agctcttgtg tatgtagcgg aatcctgcgt tcatttcctt taccacttaa
    19981 gacgtacgag acagttgctg aagaaagacc acagtctttt gcaatatctt tgattgttgc
    20041 catgataaaa tcctccttaa atagttgctt aaatttgctt ataccaataa tcttaacata
    20101 tattcaacta aagaacaata ttctacaagg tgcatatttt acaggattag aataaataaa
    20161 ttaaaaaaaa cttcttactt atgttgacat aatttagttt ataggattat aatatgctta
    20221 aataagctta tgattaactt gaattaagca aagttaagca ggaacatgca atgctagctt
    20281 gcaatgtgac tgcaaagggg gaaaaggtat gagtaaggta aatatatttg aagttggtcc
    20341 tagagatgga tttcaaaacc tgaaagagtt tttagaaact gataaaaagc ttaagattat
    20401 tgatggtctt attgcttctg ggattaaaca acttgaaatt agttcatttg taagcccaaa
    20461 agcaatacct cagatgaaag atgcaaaaga agtagcaact tattgtgtag aaaaatatcc
    20521 tgatgtgaaa ctttatgcat tagttccaaa tttatatggt gctaaagttg catgggagtg
    20581 tggtataaga gatatatcct atgttatatc tttgagtgaa actcataata aaaaaaatat
    20641 aaatcgtacc catgaagagt ctattgatga gcttaaaaaa ataatgaata cttatccaga
    20701 catgaatata tgtattggaa tggctactgt atttggatgt ccttttgaag gtttaccaaa
    20761 aattgaagat gccataagat ttgctaaacg cctttgggaa aacggtatca gggagataaa
    20821 tctagcagat acaataggga ttgctgaccc taagcaagta agagaaacta ttagtgcatt
    20881 agttgaagct ttaccagaat gcagatggca agtacatatt catgatacta gaaatatggg
    20941 aatggttaat actcttgtgg ctattgaatc tggaattact actgttcagt ctacacttgg
    21001 aggattagga ggatgcccat ttgctcctgg agcatctggc aatactgcaa ctgaagattt
    21061 agtatatatg cttgaacgta tggggtatga tacaggagtt gattttgtta aacttcttga
    21121 attatcaaaa tatcagaaga gttttgtaaa tggagtatat agtggtcatc atgtaaatat
    21181 aaagtctgat agttgtaatt gttagttcta ttgattaatc gattaaaaaa atatgctgat
    21241 tggtaaaata gatttattga taaaaaatat aaattataaa atacattatc taataaaaca
    21301 aataaaaaaa ttgaggggga ttagtatgga tattatagct ttacttttat ttttaggagt
    21361 tattattatt gcatttgtac gtaagaataa tgcaggtatt ttggctctag ctattggtgt
    21421 catagctgtt agaatttttg gaatggatga taaagaactt tatggagcta taagcaactc
    21481 aatgtttact acacttgttg gtataacact actctttgca atagtgaact caacaggagc
    21541 attaaagctt gttgcagaaa aaattgtagc tgcttctggt aaacgtgttt ggcttatacc
    21601 tatagcagtt tttatagcag gatttgttgt tgctggtgtt ggacctgggg caattccagc
    21661 acttgcaatt atcccagcaa tggctgttcc tgtagccata acagttggtt ataatcctgt
    21721 aatgcttgca cttataggtg aatgtggatt aatggcaggt cgtatgactc ctattactcc
    21781 agaaggtcaa attataacaa ccgttgcaga aggggttgga attgaaaatg ttatgcctac
    21841 tatcttagcc tgtcaaactc tttctacaat tgtattttca atagtattgt ttataatttt
    21901 caaaggatat aaacttaaaa aacctataaa tgttatagat agtaagcaaa tagaaaaatt
    21961 caatataaag cagataattt ctctaataag catagtagtt atgattgctt ttatcataat
    22021 ttttgatatg aatgttgggc ttgcagcttt tgcagtttca gctgtactta tttttttcaa
    22081 tattgctgat gaaggagaat gtatcaaatc aattccatgg agcacgatag taatggttct
    22141 tggagtaggt gcaatgatga caatcgtaga tgcagcagga ggtattgact tgatgagtaa
    22201 tgcattatca tcactgatga atagtaaaac agctactcca attatgagta tttcagcagg
    22261 acttatgtct atggttagtt ctgctttagc agtagtttat ccaactatga tgcctatgtg
    22321 tgttgacata gctaaggcag ttggaggagt agacccacta gcacttattg ctgcagttgg
    22381 tgttggaggt tctcttgctg gtgtaagccc actttctact ggtggggcat tgattttagc
    22441 agctatgggt tcaagtaaga aagactttaa taaggaagaa caaaataagg tttttgtaca
    22501 gcttttcatt atgagtgcag ttggcttatt ggttattgca gtagtatcaa tattatcatt
    22561 taatgcaata acacatttaa tgaactaatt agaataactg aaaataaatc attaaattta
    22621 acttttaata ttaatcaata taatataatt tggaggggaa gttatgaaaa aaggagcatt
    22681 aagcaatata aaggtacttg accttacaag agttcttgca ggtccatatt gtacaatgat
    22741 gttggcggat tttggagctg atgttataaa aatagagatg ccaagaaaag gtgatgatac
    22801 aagaggtttt ggaccttttg taaatggaga gagtttatac tatgcaaata taaatagaaa
    22861 taaaaaaggt atcactctaa accttaaatc agaagaagga aaaaatatat ttttagaaat
    22921 ggtaaaacat gctgatgttg tagtagaaaa ttacagacca ggtgtaatgg aaaagttagg
    22981 tctgggttat gatgagttgg ctaaagttaa caatcaaata atttatggag ctgtatcagg
    23041 atttggatgt tatggaccat attcagaacg tccaggttat gatattattg cacaggcaat
    23101 gggtggtctt atgagtataa cagggcaaga aggaggagaa ccaacgagag ttggtaatgc
    23161 tatgggtgat gtactaggtg gaatgaattt aactattggt atactcacag cactcaacgc
    23221 tagaaaaata ataggaaaag gacaaagagt agatgtttcc ctcgttgatt ctgttgtatc
    23281 gtctctcgaa actggtatac agagatactt tgattcagga caacctgaac ttatgggtaa
    23341 tagatatgct tcagcctatc cttatgactc ttttaaggca agtgatggaa gttttgtaat
    23401 aggctgtggt aatgacaaac tttttgaaaa gttatgtaaa aaagttcttt ttagagaaga
    23461 ccttttaata gatgagagat ttaaaacaaa tcttaatcgt tgtgaacatt acaaggctct
    23521 taaaccagaa atagaaaaat ggtctgttaa gcatacaata aaagaagcag ttgacttaat
    23581 atcagaggct ggagttcctg ctgctcctat ctataatctt gattgtgtta caacagatga
    23641 acatatagca aaagcaagag aaatgattgt agatattagt catcctatta taggaaatat
    23701 gaaagtcaat ggaaatccag ttaagttgat gacaacaaaa gctaaaattg atatgccagc
    23761 accatctcta ggggaaagta acgaggagat ttataatggt tggcttggtt ttgatagtga
    23821 taaaataaaa gatttaaaag aaaatggagt tatttgatta aaaaatagct aaacacactt
    23881 gtgactttag tcatgggagg ttcagttaga ataatatcaa aaaatattaa atcaatactg
    23941 taaataaaat atagaattgt cagaagcatt taagatggga aaagctactt agacattgag
    24001 tctttgtggc ttttcctatt ttcattaaga attttatatt atcagaatag atatgtacta
    24061 aactaaagta ttaacaattt aaattaatag aaaataaact acctaattat gttgaataaa
    24121 gactacttta gtgaaaataa acttatagag atgctattta aattatttta atggaaatat
    24181 caaaaatgag actgcattct caaaaatgag actgcgtttt ttaagtattt tctcgaaaat
    24241 gagacaaaaa tattgtttta tgggttttat tgaagaaaaa ttcttctgtg aattacaatt
    24301 taatcaattt taaagttgaa aattattaaa aatttagtga aatttatgta tatttcgttt
    24361 attttattaa aaataacaat tttttactaa aaataaagaa cttcaatggt gattggcata
    24421 gtacttgcta ttttatatta acaagaaatg tagaaaaaat aacaatgtat aatgggggga
    24481 atatgatgga atctgtaatg gaaaatttga acaatcttaa ggtggaggtt aaggacaaag
    24541 tctgtgttat tactataaat agacctaaag ctttaaatgc tcttaatagt gacactctta
    24601 gggaactatc acaagttatt gatgtagtat cagaaaatga ggcaatatta ggagttatta
    24661 ttactggaga aggtaaagtt tttgtagccg gtgctgatat acgccagatg caaaactata
    24721 aaagtgagga aggcagaaaa tatgcaggtt atgctcaggg aatttttgat aaaattgaag
    24781 ctcttgagaa aacagtaata gcagcagtta atggatatgc attaggtggt ggatgtgaac
    24841 ttgctatgag ttgtgatatt agaattgcat cagaaaaggc tatatttgga caacctgaag
    24901 ttaatttagg agttattcca tgttttggtg gaactcaaag attatcaaga cttgttggaa
    24961 ctggaatagc taaagagtta atttttacgg ggagacaagt aaatgcagaa gaagctaaat
    25021 ctattggact tattaataaa gtggttccaa gtgatttact tttagaagag tctatgaaga
    25081 tgatgaacca aatagttgaa aaagcgccaa tagcaataag atatgcaaaa gttgttatta
    25141 acaaagggat agatatggat ttaaagaatg ctcttgaact agagaaagat gttgcaggtt
    25201 taacttttgc tacaagagat aagcaagaag gaatgaatgc atttatagaa aagagaaagc
    25261 cagtttttga aaataaatag ctattataac taaaattgga acaatgtaga atatattcaa
    25321 aatctaaatt tcgtgaaaaa atacatatag tttaatttta aaaggagtta atttatgaat
    25381 acgggagtaa ttataagttt agtagggatt atcatagctc tatctcttct agttgtaatg
    25441 gttatgaagg gaataaatat ttttattata gcaattgtat gttcatcatt agttgctata
    25501 acaggaagcc taaatgtgta tgatgcactt aaagtggatt atatgacagg gtttgttgga
    25561 tttcttcagg caaatttctt tattttccta acagggacat taatgggaaa aataatggag
    25621 attactggtg gagcaaaatc tattgctaag atgattgtaa gatggattgg gaaggataaa
    25681 gcactacttt ctattccaat agcttgtgga atattagcct atggtggagt aagtgttttc
    25741 gttgtaagtt ttgcagtgtt tccgatagct ttagaaattt ttaaagaagc tgatttgcca
    25801 cgaagattta taccagcagc tttgactttt ggatgctcaa cttttgctat ggttgcacca
    25861 ggggcaccac aaattcagaa tattgttcca gcatcaacat tagggacaga tattatggct
    25921 ggagcagtaa atggttttat atcatgtgca gtaatgttta tagttggaag tacaattttg
    25981 tacaaaatgg tttcaaaaga aaaagccaat ggtggtcatt ttatagcaca tgaatcagat
    26041 atatttgatg aagcagaagc aactaattct aagagagaaa atggtcccaa tggtgtaatt
    26101 gccttaattc cattgatagt gtcgatttta ataattaatg taaaagttaa tggaaaagcg
    26161 atagtaccaa ttgaggtggg agtatttatt ggagctttat tagtatattt attgttaaat
    26221 aaatatcaag ataatactaa aatagttgga catgttggtg atgcatgtaa aactacagta
    26281 gtagcaatat gtaatacttg tgctgtagtt gcatttggtt cagtagtaaa atcagcagtt
    26341 ggttttgact tcatagttaa tgctatgact aatattcctg gtcctcctat tgcaggggct
    26401 gcagttggaa ctacagttat tgcaggtatt tgtgggtcag cttcaggtgg actaggaatt
    26461 gcagctccac ttcttggacc agtattttta gccaagggtg tttccgctgc agcattaact
    26521 aggactatgg caatttcatc agcagcatta gattcacttc cacacaatgg atatattgtt
    26581 actgtaacaa atggtctttg taaagaaaca cataaggatg cctatatgcc aatattctgg
    26641 gtaactgttt taaccccatt tattggaacc atagtagcag tcctactgtt tacaatgttc
    26701 ccgggattgc cataggagaa aagataaaag gtataaatta tattttgata ttataaaatc
    26761 aattttcaaa atatatacaa taaatttcat gttttaatac tgtaaatatg tatttaaaat
    26821 aacaaattga ccatataagg aggaattgaa agtgttaaat aaaccattag agggagttaa
    26881 ggttattgac ctaacgtatt ttgtagcagg tccagggact tctaaaatat tagctgactg
    26941 gggtgctgac gttattaagg tagagccaag ctttggagac ccaggaagaa agacaggggc
    27001 aacaatgaca atgccaatag atgattataa taatcctttt tacagcacat ataattcaaa
    27061 taaaagaggt ttgtctataa atcttaagag tgaaactgga attgaaatta tggataagct
    27121 tttaagcgaa gcaaacgtat ttgtttcaag ttatagaaca ggagcattaa aaagactagg
    27181 tcttgattat gaatcattaa gtaaaaagca tccacacctt atctggggtc agataaatgg
    27241 ttttggcgat tttggacctg ctaaggataa tgcaggattt gatacagttg ccttttgggc
    27301 aagaagtggg gctatgcttg atatagcaga aaaagataca agtccaataa atccacctat
    27361 agcctttgga gatgcaacaa cttcttgtag tttatctgga ggaatatgtg cagctttgta
    27421 ccagcaagct aagactggaa aaggacaaaa agtaatggca tctttatttg ggcaagcaat
    27481 atggaatgca tcatctttat tagcatctac acagtttagt gatgagtatc caaaaactag
    27541 aaaaaatgct atttcaccag taataaattc ttatcgttgt aaggatggaa agtggatatt
    27601 cttaagtatt ttagagcatg aaagatattt tgaggcactt tgtagattat ttggaagaga
    27661 agacttaata aaagaagaga aatttgcatc ttcagtagaa agtaagaaaa atgctacaga
    27721 acttattaat attttagatg gtgaatttgc aaagtttact caaaatgaga tggttgaaaa
    27781 acttactgct gctgatatag ctcatgaaaa aattcaacat gttaatgatg tagtgactga
    27841 tgaacaggct attgctaata actatatata tgaacataca agtaaagatg gaaataagac
    27901 aatgcttgca tctactccag ttaaatttgg aaatattgaa gttaacatga cttgtgatgc
    27961 tccacttata ggagaacatt cagatgagat attgagagaa cttggataca gtgattctga
    28021 tatagagact ctaattgaat ctggaattgt aacaatgaat aaagaagcat gtgaaatatg
    28081 ttaataaaca tgtgaagtat gttaataaat aggagggaat tttatggatt ttagattaac
    28141 agaagcacaa ttaatgcttc aaagagtagc aaaagaattt gcagagaatg aaatagcacc
    28201 aatagctgct gaaacagata aaacaggtat ttttccaagg gagcttttta gcaaaatggc
    28261 taaaatagga tttaatggaa ttggaacacc tgtagagtat agtggttctg gtggggcaga
    28321 tatagaaaag gtaattgttg ttactgagat tgccaaaaaa tgtgcagcat ctgcagcaat
    28381 attatcaata cacacaatat atgctcaagc tattttgaaa tttggtactg aggaacaaaa
    28441 gaaaaaatac cttccaatga tggctgaagg tgggtgtgta ggtgcttttg ccttaaccga
    28501 acctaatgct ggttcagatg ctgcgagagc tgcaacaact gcaattattg atgaagaaac
    28561 tgatgaatat gttttaaatg gaactaagtg ttttatttct ggtggaggtc aagcagaatc
    28621 attaataata tttgcattga cagacccatc aaagggaata aagggaatgt ctgctatcat
    28681 tgtcgacaaa ggtactccag gtttttcaat aggaaaaatt gaagaaaaaa tgggtattca
    28741 tggttcagaa actgcagagt taatatttga taactgtaga gttccaaagt ctaacttatt
    28801 aggaaaagaa ggtaaaggat ttaatatagc tatgacttgt ttagatggag caagaattgg
    28861 agttggagct caagctgtag gaattgctga aggggcttta gaagaaagta tcaaatattc
    28921 taaagaaaga gtccagtttg gcaagccaat ttcagctttg caaggtattc aatggtatat
    28981 agcagatatg gcgacaatgg tggaatcagc aaaattatta gtatattatg ctgcagattt
    29041 gaaagctaga ggagaaaaac acactaaaga agctgctatg gcaaaataca atgcgtcacg
    29101 tactgcaaga gaggttacaa atttagccct tcaaatccat ggtggatatg gatatatgaa
    29161 ggattatcca ttagagagaa tgtacagaga tgccaaaata acagaaattt acgaaggtac
    29221 ttcagaaata cacaaagttg ttatttcaag agcagtacta ggataggagg aaaagaatga
    29281 atatagttgt ttgcttaaag caagttccag atactaatga agttaaaata aataaagaga
    29341 caggtactct aataagagat ggtgtaccaa gtataatcaa tccagatgat agaaatgctt
    29401 tagaagaagc tctaaaaatg aaagatgaac taggagcagt gattaaggtt ataagcatgg
    29461 gacctcctca agctaaaagt gctttaaaag aagctttggc aatgggagct gatgaagctt
    29521 atttaatctc agatagagct tttggtggtt cagatacttg ggctacatct acaattattg
    29581 ctgctgctat agagaaggtt ggaaagtatg atgtaatatt ctgcggaaga caagctatag
    29641 atggagatac agctcaggtt ggaccagagg ttgcagaatt tttagggata ccacaagtta
    29701 cttatgcgaa agaagtaaag gttcaagatg acaaattact agtaactcgt tatacagaga
    29761 caggggatta tttaatagaa gcaaagatgc cagttttatt aactgctata aaagagttaa
    29821 acaatccaag atatccaagt gtcaagggaa tcttagaagc ttataataat ggagatgcaa
    29881 agattactgt attaacactt gcagatttag atgttgatac aactcaaatt ggattaaagg
    29941 gttcacctac aaacgtttat aagtcttttg taccagttaa agacaagcat aatgaaataa
    30001 tagaaggtat aaataaaaaa gaaaaagctg agaaactaat agaaatacta ttcgatttga
    30061 aactagtata ggagtgttaa gatgatgaga gcaaaagtta atcaaggtat aaatttaaat
    30121 gattataatg gtgtttgggt aataggagag caaagggaag gtaaaattaa tcctgttacg
    30181 atagaactta ttggtgaagg aagaaagctt gctgaccaat taggaaaaga gcttgcagtt
    30241 gtgatagctg gatatgaagt agagaaagaa gtgaaggaat tacttcacta tagtgttgat
    30301 aaagtatatt acatcaatga ccctcttctt aaagatttca ctactgatgg atatgcaatt
    30361 tcaattgcaa atttaatcga aagaaaaaag ccagaggttg tattggttgg tgctacttct
    30421 ataggaagag atatagcgcc tagaattgca ggtaaggttg gaacaggtct aactgctgac
    30481 tgtacaaaac ttgaaataga ttcaacagat aataagttat tacaaacaag accagcattt
    30541 ggtggaaatt taatggccac tattgtttgt ccaaaaaata gaccacagat gtcaacagtt
    30601 cgtccaggag ttatggcaaa agctgtaaga aacgaatcag agactggaat tttagaagtt
    30661 gttactccag aacttactga aaaaatgatt agaactagat tagtagagat acttccacaa
    30721 gaaaagaaat ctgtaaatct aactgatgct agaataatag tatcaggggg aagaggatta
    30781 aaaagagcag aaggatttga gcttataaaa gaattggctg ataaattggg ggcagagatt
    30841 ggtgcttcaa gagcagcagt agattcaggg tggatagaac attctcatca agtgggacaa
    30901 acaggaacta ctgttagacc agaactatac attgcatgtg gaatatctgg agcaatacaa
    30961 catttagcag gtatgagtga ttctaaatat atagttgcta taaataaaga tgcaaaagcc
    31021 cctattttta gtatatgtga ctatggaata gtaggagatt tatatgaaat tatacctgaa
    31081 atgatagaat cattaaatag gtaactttta gaaaacattg aatatgtaga atgataagtc
    31141 ttataatatt gtattgtaag acttattatt tttgctgaag gttatagtgg ggagatgaaa
    31201 caatgaaaga gcagtggtat aaggatatat ttgcaagagt attatctatg actgatgatg
    31261 gttttattgt tgtaaataca agtggagtaa taatagatat taatgataaa tattgtgact
    31321 ttttaggaaa agaaagaaaa gatataatag gacaaaatat acaaagtatt ataccaaata
    31381 caaaaatgct agatgttatg aaaaataagt attgtgaaga aggagctata catcattatt
    31441 caggtggaaa tacaaaagaa aaaagtgtaa tagtaagtag gtcttatgtg gaaaatgaca
    31501 atggagaagt agttgcagga gtagctcaag ttaaatttag attgcaatca tttgatgtag
    31561 ctaaaaagtt aatgtcagaa tatatggagc tccaatatta taaagagcag tttaaggata
    31621 actgtggttt tgacaagctc ataggtgaaa atagagactt catagaactt aagaaaacag
    31681 gtgtgaaagc ttctaaaaca aactttccag tacttttaac gggtgaaaca ggcacaggta
    31741 aagaagtttt tgctagagca attcacaata atagcagtag gtcagataaa cctatggtta
    31801 gtataaattg cgctgcaata ccagaagaat tattagagtc agagctattt ggctatgacg
    31861 aaggagcttt tacaggagca aaaaagggtg gtaaaaaagg taaattttta gttgcgaata
    31921 atggtacaat ctttttagac gagataggtg acatgccttt gaccatgcaa gcaaaacttt
    31981 taagagttct tcaggaaagt gagatagaac cagtgggagg tcttaaaact ataaagatag
    32041 atgtaagagt aatctctgcc acaagaaaga atttaagtaa aatggttgaa gagggattat
    32101 ttagagaaga cctatactat agattaaatg taataaacat tcatatgatg gaattgaagg
    32161 acaggcaaga tgatatttta ttgctagcaa attatatatt aaacaaatta aatgttgaat
    32221 acaaggaatt gaaggtttta agtgataagg ttaaaaattg ttttattaac tatacttggc
    32281 cgggaaatat aagagaacta caaaatgtaa taaaaagtgc ctatgctgta agtgatgata
    32341 tggtcattat gatgtgtgac cttccatcta agatggataa tatcagcaga gtagctcaat
    32401 gtaatgtgga tagcaattgt tcaattcatg aaatggttga gaattatgaa aagagcttaa
    32461 ttattgatgt gttaaggaag tataattgga aatgttctaa ggcagcagag gttatgggaa
    32521 tacacaagag tcttttatat aagaaaataa aaaagtatga aattgaatta aataattaag
    32581 ataacttgaa ttaaggcaat gctaaaaaag cgcccttatt caaagcgaac tgtataaact
    32641 gttttatgca gttaaaacgt gtaaaagtag atgtgaagta ctaatgtcaa tcattagtac
    32701 ttttttatta tatttataat tactatctat atttcagtat cttttgatat ctaagattat
    32761 tatgtttaat ttagtatata acaaaaccct cttagttatg gacagttttt tatattttaa
    32821 ctgtctacta agaggggata gtttagttta aatttagagt aaattttatc tagcatttat
    32881 aaaaaatatt ttgaatagct tttttgttat ttagtcataa ttttctactt ctttatcaag
    32941 ttctttgcca caagaatcac acttatatat agcattgtat ccgtaatcat attcatcaac
    33001 tattttcttt tgtctaaata caccaccaca ctcacatagg aaaccaattt ttataagtgt
    33061 aacgataaat tgtgatttta taaattttat atcattgcat ttatcgttag tgtaaaatgt
    33121 ataaatataa gagttatcgc actcatctct taattcaaat ctttcaatat atgtgttatt
    33181 ttttaattcc tcaaattctt ctttagttaa atatgatttt tgttgcaatt catgtaaacg
    33241 cttaatttgg tccattattt atatcctccc aataatttta gtattttaag cttagataag
    33301 gaatctaagt tttattaaat ttatcccaag gaattattta aatcatattt agttttatta
    33361 attacaacat atataaatta cacgaaaata aaactaattg taaaaataat atcctgtgaa
    33421 aacctaaact actaacccct tactaacccg aaaactaaaa aaagaaaata tatccaaata
    33481 taagtgatat ataaataaag atgtttcata taaatttaac tttaaatatt ctgacaataa
    33541 tatatataaa tatattatag ttaaaatata ccacatattt tgggtattgt atactggaat
    33601 tagagtaatt aatttatttt ttaacataca aatgatttta tgaaaaaaaa caagactcaa
    33661 aaatttttat attctttgat agaataaaat tgagagtaaa tttattgaat tttataaaat
    33721 ttataagggg tgagtctaag atatgcatga agataaaaat gatatatttg aatcaataga
    33781 tactgaggat tttgataata aaacagaaga agaatgttta gagctagtaa gaaaaaatgg
    33841 attgaattta atatacataa aaaatcaaac agaagagata tgtctagagg ctgttagaca
    33901 aaattgtaat gcaataaaat gtgttcagaa tcagacagaa aaaatatgta tagaagcagt
    33961 taaacaagat tggagaatgt tagaatttgt ggaagagcca acagaagaaa tttgcatgtt
    34021 ggctattaac caagatggaa cagcattaaa atatatagaa aatcagacag aggagttatg
    34081 cttaagagcc gttgaaaaaa atggtgcagc tttggagtat gtaaaagaac aaacagaaga
    34141 aatatgtata gaagcagtta gaaacagcga atttggattg gcaagagtga aaaatcaaac
    34201 agaaaaaata tgtatggaag cagttaagca ttgtagctat aatctaaaat gggtagaaaa
    34261 tcaaacagaa gaaatatgta tggaagcagt gagacaaaat gggcttgatt tgaaatttgt
    34321 aaaaaatcaa acagaaacaa tctgtttaag agcagtaaga caaaatggaa tggcattaga
    34381 atttgtaaag gaacagactg ttggtatatg cttaaaggca gtaagacaat atggaatggc
    34441 attaaaattt gtaaaggaac aaacagaaga aatttgtaca gaagctataa aacaagataa
    34501 aagagcactt agttttgtaa aaggagataa ggaaaaatat aaggctctat atgataataa
    34561 tgaaccattt gcaaagagat atgttagaaa tgtaatagaa aaagaaaaca gagcattgat
    34621 taaaaaagga gaagaaaatg caaaaatcaa aattgcagga aaactttatg atagaggcat
    34681 gtcttttgaa gatatatcag atatagttga gattgaaata agcaagttaa aagtgagttt
    34741 aggtgttttt taaagtaata aatgtaggtg ttttttatgg gaaatagtta ataaaaaaat
    34801 aatcgactag ctctttgtag gacagcaaaa ttagtgatat aatttgatgt ataataaatt
    34861 atttttatta gataaggggg aattaaagtt gaagattaca gtattatacc aaacaagaag
    34921 tggaaacaca gaaagggtcg ctaagctgat agaagagggc gctaaaaagg tggatggagt
    34981 agatgtaaag cttatgagac ttgataatat tgatttagat tatttaaatg aaagtaaagc
    35041 agtaatattt ggaacaccta cttatttagc aaatacaact tgggaagtaa aaaaatggtt
    35101 tgatgaagat tcaaaaaaag ttaatttagc tggaaaatta ggagctgttt ttgctacatg
    35161 tgattatata tgtggaggtc cagatgttgc gatacttact atagtaggac atttgatggt
    35221 taaagggatg cttgtgtatt ctggaggtgg ttctttagga aagccattta tacatttagg
    35281 acatgtacat tcaattgaag gtccagagtt acaagatgag aaggctataa tatttggtga
    35341 aagaattgct actaaggcaa aagaactatt tgcttaattt ctatatgcat agtaatgatt
    35401 agagtaaatt tagggtttta atggagattt gaagtaaaag ttataaaata aaaaattttc
    35461 cataaaatta ctcaagatga attataatat aattataaat aaattatatt attaaaatat
    35521 ttaaaatcaa acgtcaatag cgagggaagt ttatggtatg gcatatagaa tatgagattt
    35581 tttctgcaat tatagttatt tttttaatgg tatacttttt tagaagtaaa tttataccta
    35641 ctttacaaaa caaaatatat tgtgcacttt taatattctc atttttattt ataatttcaa
    35701 atattttagg ttcattttgt ctaaataata tagacaaaat tccaattttt attacttttt
    35761 tattgaatca gatatactta ttgttattgc caataccagc agcacttgta tcattttatg
    35821 ttatggctat tatttatcaa gatataaggt atatgaaaaa aagattatta tttttatcta
    35881 taccacttat aatttgtata atcttatcta ttacaaatcc ttttacgcat attctatttg
    35941 gattgtcaaa agaaacaggt tatatacgag gttgtggata tgttgcaact tttgcatctt
    36001 ttttctttta tgcaatatat tcagcatttt tatcttttaa atatagaaaa gcaatgcatg
    36061 aatcaaaaat atgggctatt agaggttttc ttatagtttc aattgtggca attatcttac
    36121 aggcaatttt tttacagtat cttcttacag gaactgcatg tgcatgtagt ttacttttga
    36181 catatctttc tatgcagaat cgaggtctta ttattgatga ccttactggt gtactcaata
    36241 gacaatcttt tattcaagag ctagatttga atatcaatac tgaacaatca ggatttatta
    36301 ttacaattgc gcttgatgat tttaagttta tgaatgaaac ttttggaaca aaaaatggag
    36361 atattgcact aaaagaagtt ggaaaatatt tgattcagat acatgatgta aaccatgtat
    36421 atagaatagg gggagatata ttttctattg ttgtcaatag tcaattaggt atagaaccag
    36481 atgatattat cagaaagata gaagaaagat ttaaaaagcc ttggctagta gaagagataa
    36541 gttttaattt agcaactagt attgctgttg tttattaccc agaaaatgca gaaacgacag
    36601 aagatgtaat tgttgctata gatttctcta tacatgaagc aaaacgctct gagtcaaggc
    36661 gagttgttta tgcagatgcg tctataagtg aaaagataaa gaagaaacat gttattaaaa
    36721 attgtttaaa agaagcatta aaaaatgatg ggtttgaagt gtactaccaa cctatatttt
    36781 ctaatagcag gggcaggttt acttcagcag aggcattact taggctagaa cacaaagagt
    36841 taggttttat acctcctgat gaatttattc caattgcaga acaaacagga ttgattaact
    36901 caattggatt agtagtcttt gagaaggtat gtagatttat agctagtgat gagtttgaag
    36961 atttaaaatt ggataatata gctgtgaatc tttctgtagt acaatgtatg caaaaaaact
    37021 tagctgatga tttactttat cttatgaata aatataatgt atcaccaact aaatttaaac
    37081 ttgaaattac tgagactgtg gctatgggct cttttaatat aattaaagaa acaatggaaa
    37141 gattaattga tttaggtgtt aagtttgcac ttgatgattt tggaattgct tattctggag
    37201 ttactaatat gctttcttta ccattttcag taattaagtt agataagagt ttgatttggt
    37261 ctatgaatga agatagtagg cataaactta cagttgagac gattatagca cttataaata
    37321 aattgaatat gaaggctgta gcagaaggtg ttgaaaccat taagtgtgct gaagatttaa
    37381 tagctatgga gtgtgaatat ttgcaagggt attatttttc taaaccagtt ccagaagatg
    37441 tttttaaaaa tttgttgaaa gaaaatactt ttacaagtta acacccttta tttttttaat
    37501 tacaactttg ttcattgaca ctgccagcga tatattataa aattaacaca ataataaata
    37561 ttaaatttat tatacatatt attaatatta attaatggag taaagattta ttatatattt
    37621 tatttaatta tcctaaagtg agaaaggaag atgtgacatg aataaaaaga aaatattagt
    37681 gccaattgat gggacagaaa gaagtatgca ttctttggaa tttataaaag gaatatttaa
    37741 aaaagatgaa gttgaagtag aaataatgaa tgtaaaagaa ctagtattta ttgatggcat
    37801 atccctggct gaagagataa aaaattctga aaatttagga agaagaatct tggataaagc
    37861 agcagagata atgggagagt atgatgttaa agtacatttt acttttggat atccaggaga
    37921 tgagataatt agaaaagcaa aagaagataa tgtagatttt atagttatga caaaatctac
    37981 taaaaaaggt cttacaagaa tgataggttc agtaacagca agtgtagtta aacaagcaaa
    38041 atgtatagtg atgattgtac cggagtagaa ttagaaaggt atttgaaata tgaataaaaa
    38101 gatagtagca gtttgtgaaa gtgaaagaag tttaaattct cttaagacag ctgcaaatga
    38161 tttaggattt gatttagttt atgaaattca agaaaatgat aatataaaaa atgaactttc
    38221 tatttatgat atagaagatg catccatagt gttatttgtt acaagtgatt ctatagaaag
    38281 catagagaag attgatagat ttatagatag agaattttat gaagttgatc caaagtatat
    38341 aatagaggat gcaaaatcta tagtaagtga aattttaata gacttaaatt aatcgtgggt
    38401 gtcgcactaa gaatagagcg acgccttttt atatatttta cacaagaaat aaagaggttg
    38461 tatcaaaata tagataagag tctttttcat tgttgaaatg atgaaaatat atttgatttc
    38521 atacaaaaaa gagtacctca taactaaaaa gttcactttg agacactcca tttttatatg
    38581 ttagttgcat ttaaggtaaa atagaagttt aatcatgttg aaataactga attacatctt
    38641 ctaaatgatt gtacattgat gtataaccta agtaagagtc tttactatca aatgcaatca
    38701 ttatcttgtt atggtggcta atcatatcct tgagagaatt tctatattta aaaccaaagt
    38761 attggttaga agaaactgtt ttaggtatta actcataata ttttataaaa attggattat
    38821 gagaagctaa aattatctct ttgtgaaatt gcaagtcagc atcataacat tcttctatca
    38881 aaccatgaga aaccttatcc aattcctcta ttgactttag gtaagtatat tttatttttt
    38941 gaagttcttt agatgtagca tttaaaattg ctagtttagt agcttcgctt tcgataagtt
    39001 gtcttgctct taaagattct acataaaatt cttgtatgtt atgattttta tagatttcac
    39061 catcatcaaa taggtcaact tttttaaatc cttcaactat aggacttgaa taaggcacag
    39121 ctctaagtat tccttcctta cagagtatat ttatggcatt tcttacaggt attctactta
    39181 tgttaaatag gtcagcaatc tttctctcac ttggtaattt actattaatt ttaaaagttc
    39241 catttagaat gtttaattta atataatcaa taattttttc agtggcattt aagcttttca
    39301 taaaaactcc taaacatata atttttttat gattgtatta tatcatagtt aagagttaaa
    39361 tctacttttt tataatatgt ataagacagg atagaggcca aaatagctat aatagccaag
    39421 ataaagtatc catattgtat tgaagtatat tccgatattt taccaactat cattggaaat
    39481 acagacattc ctatagcata aattgcttga aaaaaaccca ttgcagtaga tttctgatgt
    39541 tttggtacat ttttcataga ctcgctagta aggtaagaaa ggagtatacc tgtagatata
    39601 cctggtagta cttgtagtac aaatattata ttaatatttt ttattgttgg aactaaaaag
    39661 caataggctg aaactaaaat aaaaactaaa ggaatccaaa attttggtcc atacttagta
    39721 caaaatttag tagaagcaaa gccagaagaa gcaacagcac acaccatgta aaatatagaa
    39781 gatagaccta tgagggtaga actagaacct aagtttttaa gaatttgagt agtaaatgac
    39841 attgttgtag acatttgaat accctgttga attaatgcca taaaggaaaa aaatacaagt
    39901 cttttatcct tgcagataga taaatatttt ttaattgata ttttttcagg tattttttta
    39961 gaattatgat tttttatatt taaggctaga atcgttccaa taactgaaga caatacactc
    40021 atgatacata aggctgtcat tcctaatttt tcataaaata aagtactcat aacaaatcca
    40081 atcaacattc ctagattgtt taccaatatt atcgtacttg tagctttttg ttgatgttct
    40141 gtagaaaaaa agttggtata gtgaaccatg taagaaatcc aagttgatga tgatattcca
    40201 gataaaatgt tagctataag aaaaccattg cctccaggag aaaagactct tataagagat
    40261 gctaaaccag caaagaaaat tccagacagt ataaatattt tatgtttatt tatggaatca
    40321 gcaaatactc caacaggaag ccttaataaa cattgtgata ttccatatgc acctattatt
    40381 ataccaatca tatttgtaga tacattacat gcacttaaat atggattttg gaaaggtata
    40441 tatacatatt gtgaaaacca gaaaaatgta acaatagaaa gtaaaagtaa tttttgatta
    40501 gatatttttt tcatattatc ctccatgtgg tatttttata atgcaaataa tataccattt
    40561 agatttttta atcaagtact ttctctatta aaaaaaattt aaatatataa ttattattta
    40621 caaaaaaatt atactttata gttgaattaa gaaaacgctt atgatactat aaagttaaca
    40681 aatttgaata tataacagta gacgtgttac tggtaatgca ggcaagatcg aatcaatttt
    40741 tatagattat ctatagagta ctatgggtat aatctattta tttggttcga tttttttgtt
    40801 taattggcca taaaacaaaa agcctatagt tgaaaatgga ggatttttta tggattataa
    40861 aaatatagct caagaaattc tactaaatgt aggaggaaaa gagaatgtaa atgaagtgac
    40921 tcattgtatg acaagactta gatttaaagt caaaagcgct tctaaagtga ataaggataa
    40981 attatctaaa actgaaggag taataactgt tgttgagagt atgggtcaaa tacaagttgt
    41041 aattggaaat aaagtaaaaa aagtatatga tgaggtcata aaaattgtac ctcaatcaaa
    41101 taacataggt aaaaagaatg aaagtcaaga aaagcaaggt ataattaatt ctatattatc
    41161 agcagtagct ggtatattta cacctacaat acctgctata gcaggggttg gtatgattaa
    41221 aggtatttta tcagttcttg ctatgtatta tatgaataaa aatggagtag atataaaaga
    41281 aacgcaaagc tatattatct taaatgctat ggctgattct atattctatt ttatgcctat
    41341 tatactagga tatactgcgg ctaaagtatt taatgcaaat aaaataatat ctatggtatt
    41401 aggagctaca ctttgttatc caacttttac tgctttgatg gcaggtgaag aatctgttag
    41461 atttttagga ttggcagtaa caaaagctac gtatacatca tctgttattc caataattat
    41521 tgctatatgg gctctttcat acgttgaaaa agtattagaa aagtatatac ctgaggtaat
    41581 taaaataata atggttccaa ctttatcatt ggtagtaatg ttacctgcaa cattattttt
    41641 atttggacca atcggaattt atataggaaa tgtcataaac ttttcttata agtatattta
    41701 tgaattaagc ccagcattgt gtggagcatt tgtaggtggg ttatggtgtg tgttagttat
    41761 atttggtgct catagagcat tattaccaat aggtataagt gatgttgctc aaacaggaag
    41821 acaaaattta ttggcgtttg caggagctgc aaacttttca caagcagggg ctgctctagg
    41881 agtattcttt aaaactaaaa atcaaggact aaaaacaatt tctatgtctg caactataac
    41941 agctttattt ggtataactg aacctgcaat atacggtgcc aatcttagat taaaaaaacc
    42001 tatggtatgt gccgtaatat gtggtgcatt tggaggtgcg ataatgggaa tgggtggagc
    42061 ttatggtaat gcatttgcaa accaaggtgt tttaactata cctgtatatg ctgaagcagg
    42121 tgcattaggt ttcttaagtt atctaggtgg ttgtgctata gcattttttg gttcagctat
    42181 ttcaacttat ctagttggat ttgaagattt ggaagagtct tctaataagg aaaattcatc
    42241 tataaaagta gaaacgaagg atggtgttat tgatataact tcacctgtag agggcgaatg
    42301 tatagaattg tcagaagtaa aggatgatgt atttgcttca aaagctatgg gagaaggtat
    42361 agctgtgtta cctacaaaag gagtaataac agcccctact gattgtgaag tagcttcttt
    42421 atttccaaca ttacatgcaa taggattaaa attagataat ggagcagaaa tgttaataca
    42481 tgttggaatc aatacagtag agttaaatgg taaatacttt acaaaacatg ttaatcaagg
    42541 agatttagtg aaaaaaggtg ataaacttat atcgtttgat atagataaaa taaaaaaggc
    42601 tggttatgat gtaactacgc cagttatagt aaacaataca tttgattttg ggcaagtagt
    42661 ttcttgtaaa tctagttatg tttcaaccaa tgataatata atttcattag tattagctgg
    42721 taattaagtt aatttgacta gagataagta aattgattta aaaggaaata agtaaatcaa
    42781 caattaaata catctaaaag taagtttatt gcaatgaatt gttaaagaaa ataattaata
    42841 tggagtaaat ctattgcaat gaagtaacaa agaaaataat taatatagag taagtttatt
    42901 gtaatgaatt gttaaagaaa agagttaata tagagtaagt ttattgtaat gaattgttaa
    42961 agaaaataat taatataggg taaatctatc tcaatgaaat aacaaagaaa agagttaata
    43021 tagagtgaac ttattgcaat gaatcaacaa agaaaataat caatataaaa aggagaaata
    43081 tttatgaata caggatttcc aaaagacttt ttatggggag catcatcttc tgcatttcaa
    43141 gtggaaggag cttgggataa agacaataaa ggaaaaaccg tagccgacta taattctttt
    43201 aaaaagtctc atttacaggc agatactaaa gtcgcatcag acttttacca taattatgaa
    43261 gaagatattg aattaatgaa agaattaggg atgaaaacat atcgtttttc aatttcatgg
    43321 gcaagaataa taccagatgg tgaaggagag ataaaccaga aaggtttaga attttataat
    43381 aagataatag acaaactgat agagtgtgat attgagccat tcgtaacttt ataccatttt
    43441 gaccttccat ttaaattagt agagaaatat aatggttggg aaagtagaga aactgtctat
    43501 gcttttgaaa gatttgctaa aatatgcttt aagcattttg gagatagggt aaagtattgg
    43561 caacctcata atgaacagaa tttaattgtt agagtggagg agagaattaa tatatatgat
    43621 gaaacagatt catggaagat agataaaatg cgtgctcaga tggattataa tctttgctta
    43681 gcacatgcat tagcagtcaa tgcatgtcat gagatgataa aagaaagtaa aattggagca
    43741 gcagtatcat catctgtaac atatcctctt acttctaagc cagaagatgt atatgcagct
    43801 agaatgaatg ataattttaa agtgtactat atgcttgata tgcatcatta tggagaatat
    43861 ccagggtatt atatgaagta tcttgaaaaa agaaatatag ttcctcacat ggaagatggg
    43921 gataaagaga tacttaaaaa agctaaaatg gattttatag ctgtaaatta ttataggaca
    43981 aattgtgcag aagctttacc tgaagattca caacatccat ttggattaag agaaggaact
    44041 gttgatttta gtatgtatgg actatttaaa atgtcaatga atcctaactt agaggcttct
    44101 gagtatggag cagcaataga cccatcaggt cttagagttg ccttaaatga atattggcaa
    44161 agatatcact tacctgttat aataacagaa aatggtctag gagctaaaga tatattagaa
    44221 gatggaaaaa ttcatgatga ctatcgtata gactatttaa gaagtcatat aaatgcatgt
    44281 aaactagcta ttgaagatgg tgtagagatg attggatatt gtccatggtc atttacagac
    44341 cttttaagtt cttctcaagg ttttaataag cgttatggac ttgtttatat aaatagaact
    44401 gaccatgaag ttttagattt aaaaagaatc aagaaagata gtttttactg gtataaagaa
    44461 gttatagaga acaatggaat agtaaaataa acataaatat atattgaggg ttttagtatg
    44521 tacattacaa aggtattaaa taattcgttg ctacttgcaa aggatgataa tgatgaagaa
    44581 ataatattga tggggaaagg cattggccat aattataagg ccgggtctga gttaaataaa
    44641 aaagatatag aaaagatttt tgttcttcat gatgaaaata taaaaaagag tattatacaa
    44701 ttggcaacag aaatagatgc agaatatttt ggtatagctc aaatgataat tgcatatgga
    44761 atagaaaaat ataacttaaa acttatgaac catatatatt tggccttaac tgaccacatt
    44821 gcctttgcag tgagacgttt taaagaaggt ataatgattg aaaatcacta tttgttcgaa
    44881 atcaaggaat ttaatccaaa agagtatgat ataggaaaat atgctattac tgtttttaag
    44941 gaggttcttg gtttagacct accagaagaa gagattggta atatagcagg ccattttata
    45001 aatgcacaac aagataatcc ttatagtgat agaaataaga gaagtgctaa aattgtaaat
    45061 gctattttgc aaatagttca ttatcacttt tctattgtat ataataaaga gtcattttat
    45121 tataggagat ttgtaatgca tctaaaggcc ttttcacaaa gatttctggc taatgaacca
    45181 tcaagagaaa aaatagattt tatatatgag caagttcaaa aaaattgtaa ggaagagtat
    45241 gaatgtgtga aaaaggtagg agcttatatg tataaggaat actccagaga attaccaaga
    45301 caagaggagt tatatttgat gattcatatt cataagattt taggagaatt atgcgaaaat
    45361 agttagaata tcttaatatg ctagtaatat tataaaaaat taaaaattaa ctgcacttta
    45421 aatatgaata attgcattac ttaagggggt tgtcttaaaa ttgatttttt taattaaatt
    45481 tgaagctcaa ctcctttttt aaacctttat atcattacaa ctttaatgtt tatacgactg
    45541 tactttagat gtatatctac tttttacatt tctcaacatg caaacctacc attttttgca
    45601 gtatgttaat tgaaataaac cattgagtta accctaaatt tattctggac ttataggaat
    45661 agttccaaag atactttaaa atattttctg gaataattgc ttatttcaca tatgttttat
    45721 tgtgatgttt tgatttaata aattcttcaa ctctgtatat agtaaatgta ttatcatgtt
    45781 tgttttattc aagcacactt ataatacgct tgattttttc acaatgatga aatatactaa
    45841 tgggtgttat aggtataaga atcgcattac acttgaagaa gtaagtgata aatgaggtat
    45901 aaatcctcat atgataaact actatttcat tgaggaacat attctaagtg atgtcaagat
    45961 actacaatat gactaaattc ctagtgataa aaaagtctac gaatgagtgt agaaagggga
    46021 actaagatga ataaagtttt aattatagat gatgataaag atctttgtat tttgttaaaa
    46081 aagagtattt caattgaaca gattgaagca aactattgcc attctgggag agatggactg
    46141 gttgaacttt caaaacaaga ttatcagtta gtagttctag atgtgatgat gccaggtatg
    46201 gatggttttg aaactatgga acaaatacga aaatatagca gtattcccat cttaatgctg
    46261 acatcaaaaa gcgataatta cagcaaagtg catggattgc gagctggtgc agatgactat
    46321 ctaaccaaac catttgaaat tacagaattt actgctcgtg tactgtcttt aatccgacgt
    46381 tacacccgtt ttaatccaaa aaatgaggag ccacagcaac tttctttcaa aggtttgttg
    46441 attgaccttg accagcatac agttactacc aatggcgaac ctatgttgct tccacccaag
    46501 gaatttggtg tacttcttta ctgtgcaagg catcagggta agattttgac aaaacagcag
    46561 atttatgaag ccgtgtgggg tgagccatat gtatatgatg acagcaatat catggctatt
    46621 atcagtcgcc taagaaaaaa aattgaacta gacccatcat ctcccatcta tttgcaaaca
    46681 ataaaaggaa ttgggtatca attcaacacg gaggtatgaa aatggatgaa caaacgataa
    46741 ttcttttctt actcttgctg tcgattggtc tactttttct ttccatattt gctttttcca
    46801 agcttgggca tatttacaag cgcctaaaag atatagagga gatactggct gatgtacaaa
    46861 ttggagacga aaatcgtaaa atactaatta agccatgtga tgttatggct ccattggttt
    46921 atcagcttaa tgagattgtc tacgattatg aaaataagtt actttctctg aaaaagtcgg
    46981 ataaagctag caaacaactt atgacaagtt tatctcatga tgttcgtaca ccattaacaa
    47041 ctctgattgg ttatctggat gctgtacaca gtggtatagt tatcgaacaa gagcgggagg
    47101 aataccttga aatcgctagg cgtagagcct acgacctcaa ggattacatt gatgttcttt
    47161 ttgactggtt tcggttgaac tctgatgaat ttacgctgtc aatagaatct gtggaaattg
    47221 cagagttgtc acgcaatatt ttgaaagact ggattcctat attccatgaa aagaaactga
    47281 attttgaaat tgatattcca gaaaatcgac taatggtaaa cctcgaccca gatggatatt
    47341 cccgtgtggt caataatctg gttcaaaatg ttcttgctca cagtaaggct cggcaaatca
    47401 agattaccat gagtgaggat tccagaatgg tattactccg tgttgaggat gatggtgttg
    47461 ggattgccag agaaaattta ccacatattt ttgagcgact gtataagtgt gataaaggac
    47521 gttcagaaaa aggaagtggt cttggtctat ctattgtcag tcaaatggtg gagcgaatgg
    47581 gtggtcaggt ttccgtagaa agtgagattg gaaagtatac cgtatttaca gtgagcttac
    47641 cattaatata aaattgtttt tcccccatgt gacatgtata cacatgggga ttttttgtaa
    47701 gttataaagt tgcaaggtta atacaaggtt gttgcaaggt taatgtaaga ttgaggtggt
    47761 atgataactt taataaacaa aggaggcttt tattatttat gaataattta attattgaaa
    47821 caaaaaatct cactaagcaa tatggtgctc aaaagagcgt ttctaatttg aatattcacg
    47881 ttcagaaagg tcgcatttac ggattgctag gaagaaacgg tgcgggtaag acgacaacta
    47941 tgaaaatgtt attgggtcta acaaaaccaa ctactggaga aattaaaata tttggtcagg
    48001 acattcacaa taacgagaag aagcttcttc ctcgaattgg atgtttgatt gaatcaccag
    48061 ggttttatcc taatctgaca ggaacagaaa atctcaaaat ctttgcaagg cttagaggaa
    48121 taccaaaaaa aaatgcagta gaatcagcac tggatgtagt cggtttgcca aacaatgaca
    48181 aaaagttatt ttctcaatac tctcttggta tgaaacagag gttggcgatt gctcttgcca
    48241 ttatgcatga cccacaactg ttgattttgg acgagcctat caatggactt gatcctattg
    48301 gtattgcaga agtaagaacg tttattcgtg atctctgcac agaacgaggt aaaactattt
    48361 tgatttccag tcacattctt tctgaaattg ccttgttggc agatgacatt ggcattattg
    48421 accatggtat tttgttggag gaagaaagtc ttgcccaatt agaagaaaaa aacagtaaat
    48481 acatacattt tgctgtatct gatgcagcac aggcagcaag gcttctggaa acccaactta
    48541 acctgaaaaa tttcaaagta gcagataatt acaatattat gctatatgat acagatgttt
    48601 ctgttgcaga aataaatcgt gctttcattc taaacaacat tgaggtatca gaatctcatt
    48661 tatgcgaaga tactcttgaa gattacttca agaaagtaac agggggtgaa ggaattgctt
    48721 gatataattt catgtgaatt ttcaaaactc aagcggttaa aatttatact gatttctatt
    48781 ttaggtgctt gcttatttcc aattcccgca acaattttga ttgcaaagga caacttgccc
    48841 tttgagcaat tgttcaaatt agttgtcaat tttggatatt ttttgctatt accaattgta
    48901 ttaagtattg ttgctagcca actatttttt atagaaagag ataatgatgt attaaaaaat
    48961 cttgcaacag tgccagtgca aaaaggaaaa ctggctctgg caaaattggc tgtgttgctg
    49021 tttatatcat tgttctactc tgttgcaggg ttaggagcta ctatcattgg aggtttcatt
    49081 gttggaatag tagagggggt tgctgtaaaa ttaggaatga gcattgcttt tggaattatg
    49141 ttttttgtta ctgtacttcc tgttgtggta ctcattgtat atttcaatcg cagctatatt
    49201 ttttctatta tactttcttt tgtatatgct attttcaatt ttagtatatc cttaaatatt
    49261 atcaattttg aaccaaataa cccaatatta agtgtattac ctgctcctgt tataatgcgt
    49321 tggtggatgg cattttgggg agatcccact ggcgaataca cggcacttag acagccatat
    49381 ttgctctcaa ctcctgcttg tgtaggtatt ttatgtttga taaccattat agcagtcctg
    49441 ctgatttgta tgatatacaa aaagcaggag aattaggagg tatctatgct tgatataatt
    49501 actacagaat ttcaaaaaat aaaaagatat aatattctat ggattggtgt tgttgctgtt
    49561 ctgttttcag cactattggc agtttttcag gttgtaagtt cacacggcag tgaccctcta
    49621 acgtatgaga gctttgcaaa cggagttatc tggaataatt tcagtcttag ttttccattt
    49681 gggattacac ttattggagg atatcttatt aaccgtgaat atacagacca aaccttaaaa
    49741 aacatcctaa ctgttcctat ttcattacgc aaattgctag tgggaaaatt aattgcggtt
    49801 ggaggaatta ctgtattgtt aagtcttttc agcttcttat gcacatttat tttggggttg
    49861 ttgttttgca atatcaatat atctgttaca ctcgttgtaa aatccttagt tcagattttg
    49921 tctgttaatc tgtattgtta tgttgctgtt ttgccgatta ttgcttattt tggtcgtaaa
    49981 caaaatgcat ttctgacggg tgtagggatt gcatttgtat acggtttttg tggtgtgttt
    50041 gttgctggaa gaaatctgac agacttttat ccaatcacag caggtcttgg tctagttgga
    50101 tacaacaatg gtgctggtac tgtttatcag ccacttatcg gtgctatgac tttgattgta
    50161 attctaatct tgacaactat tctacttgta tttacaccta attatgataa agttatgacc
    50221 atatctaaga aaaaagggat aaagcataaa aagtattctc ataaataaca taaatattgt
    50281 ttctattatt taagttgcta gacagtgaaa agaaaaaatc tgtcgtacag tgtctatatg
    50341 ctcatgtatt caagaagcta cagttttgat ttttcataac tgtagcttct ttttgtattt
    50401 attaaatcac gactatcata atacttgcaa taggtatagc gacaagagag agtactgtca
    50461 tatgtgtttt atatattaca ttaagtatat tctaaaatat atgttttaaa taatatattt
    50521 gaaaatagga atatttaaat aaa
//
LOCUS       DAMJVK010000017        50427 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.017, whole genome shotgun sequence.
ACCESSION   DAMJVK010000017 DAMJVK010000000
VERSION     DAMJVK010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 50427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50427
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.017"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            132..971
                     /locus_tag="PC414_000990"
     CDS             132..971
                     /locus_tag="PC414_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010293327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2463399.1"
                     /translation="MKDYYKIGEISKIYGIGRDSLMYYEEIGILRPVRDINGYRMYNI
                     SDIWKLNLIKEFRSLNFPMKKIKEYLDDRSIESTKNILNEELDLIDKKIAEFISHKEN
                     IIKRLSSIESVIQNTKIDEIEVVYIEKRKALELNADIKRDEDFDFLIQKLQKEYEDRF
                     NILGNNNIGSAFSTEAINKGIFNEFKSVFCFLESNEKVYNIVFDEGYYVTLNYTGSNS
                     NNKMYMEKVFKFIEENNYKIIADPIEIYKIDIHETGIVEEFVTEIQVPITKYLYFNGL
                     DGK"
     gene            1086..1325
                     /locus_tag="PC414_000991"
     CDS             1086..1325
                     /locus_tag="PC414_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463400.1"
                     /translation="MKCSNCGSVNIEKGILTTGGIYAETIGLKYRGDGKLDKVINKTF
                     PVQSTIYSDLCLDCGEIVRTYIKDNTDRNWCKDKK"
     gene            complement(1496..1672)
                     /locus_tag="PC414_000992"
     CDS             complement(1496..1672)
                     /locus_tag="PC414_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463401.1"
                     /translation="MFRDEMDKCTHMLTAYISSLYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(1726..1822)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(2177..2851)
                     /locus_tag="PC414_000993"
     CDS             complement(2177..2851)
                     /locus_tag="PC414_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDF2463402.1"
                     /translation="MNHHILIQFEYIARIITAGFCGALIGYERKNRLKEAGIRTHFIV
                     ALASALIIIVSKYGFNDIVGTPGIGLDPARVAAQVVSGIGFLGAGLIFIRNQSVSGLT
                     TAAGIWATAGTGLAIGSGLYLVGIVSSIIIVIVQITLHRDRKWMKLPTAEQVSLKISN
                     STDSIEYIEGKFKKSNIEIINMNIKRLDGDWLEVVIYTKLPKGYKKTNLLKLFSDNPL
                     IETLEI"
     gene            complement(3071..3625)
                     /locus_tag="PC414_000994"
     CDS             complement(3071..3625)
                     /locus_tag="PC414_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDF2463403.1"
                     /translation="MSIALILIDIQNDYFKNGKCELFQSEETAENAKKILLFFRKNNL
                     PVIHIQHISTDKTASFFLPNTHGSEINKIVFPLDNEEIIIKHTPDSFFETDLQSILEK
                     KNITNLVICGMMSHMCIDTSVRTAKKLRYDITLISDACTTKNLSWNNIEINANTVHQV
                     FMASLQNSFADVKNTDKFLSNYYK"
     gene            complement(3703..4068)
                     /locus_tag="PC414_000995"
     CDS             complement(3703..4068)
                     /locus_tag="PC414_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2463404.1"
                     /translation="MKIRAEYTCPLEIVHDIIKGKWKTILIFELRQSNKTFSELQHNI
                     YGINQKMLIQQLKELRDFGIIDKISSAGYPLHVEYFLTNRGKKMLKAVEIMQEIGIEY
                     MLEHGQQEILDNKGICYNK"
     gene            4533..5318
                     /locus_tag="PC414_000996"
     CDS             4533..5318
                     /locus_tag="PC414_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2463405.1"
                     /translation="MIVKIDNTLEKEFWQYVSHEESLNLFIIGYVENYGFSSQYQDIW
                     SQVEGGNITSIILKNKSTLIIYSFKNNFNIGEMKNHIKDLDVESISGKKCVIDRLISK
                     YKDFYEKLDNKFCVLKEIKEIDFSNMKEYKIENAQEKDIDEIGKLLNRSDYKVSKNYI
                     EERKVHLKEGNVRAYFIRNDDTMISTVSTGMETSFLAMVVSVSTDKRYRGKGLASYMV
                     YNLSKELLLEGKVPCLFYNNDVAGKIYHNIGYKEINEWTILFK"
     gene            complement(5415..6239)
                     /gene="pdaA"
                     /locus_tag="PC414_000997"
     CDS             complement(5415..6239)
                     /gene="pdaA"
                     /locus_tag="PC414_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HDF2463406.1"
                     /translation="MKKDSLKKYIMIGAFALTLFGIASINFKSLDKTKTQISSPTLDT
                     HEYDWYFNPREDGKQPSPIKEADFFKKYGAYYVGNPNEKVIYLSFDAGYESGNTPKLL
                     DTLKKHNAKAQFFVVESYIKSNPELIKRMEKEGHLVCNHSKSHPSMAGITDFEKFKEE
                     ITSVEKAYKDVTGKEMPKYFRPPMGKFSEQSLKYTQDLGYKSIFWSFAYVDWYEKKQP
                     THEFAKNKIYSRTHPGAIVLLHPNSSTNTEILDEVLTHWEKEGYKLKTLDYLNNKK"
     gene            6460..9579
                     /locus_tag="PC414_000998"
     CDS             6460..9579
                     /locus_tag="PC414_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDF2463407.1"
                     /translation="MDNIISVLNDVVFLDIEVSGLDCLNSEILEVGAVKVKDWKIYTY
                     ESLIKNKFEVPIEVFSVCKNLDKNDLEIANKIELVEDRLVNFVEDSFIICHDLSLKKK
                     FFEYHMPKLKNKFIDLIELAVILEPYHKDYSLEYLKNTLTNCNSKVENRALSDAIDII
                     NIVNCLLVKFNNYEKTTLEPLSFKINSYLKKFNLPTWEWSKFLEEANYDLSNNINIKK
                     EYNIFDSKEEKKKERETLKILNEEEKNYEELLKYKTIWENKEGFTYEYRPGQYELTKT
                     IRELFRNSEDEEKIACIEAPTGIGKSVGYLLPAILEARINKKRLLISTDTKELQIQLI
                     NKDIPNVLDSLGLNGKVSYGYIKGKNNYICIDRLEAYIDDYESQNPTKGEILSLIFLK
                     RLVEGGKYGDIEEINYLVFDNFKEISTHLRNVSCDPNMCRPKKCKKDCLYKNRIEELK
                     EEHITVVNHSLLAKWPYKDEKPLENIIVDEAHNLTEKGYDFFSSIINSKSLRYLLQEI
                     YPYEFIQNSSFIYKKYSRNMRKIKAFDKFYNVLKIGREDKQKIARSINFIIEEIDSIL
                     KFGNCNEYNNVSNYNLRWELNLQIDEIVGKLKKDGIDTEISYRAYSEKIKLSCEKIIK
                     NLVSIIIIIYRNIDDDSIDKEADIYKFGKAKTRDLEDIKIIFEIFLEYDEKDDYARIV
                     EIDKNYNDFEFRVVPLKIADLFEENILSQLEKGIFLSATLSLSESMSYFKNTLGIDRV
                     KNVEKIIEPIFDYKNRVSVVGFSDICEYRNSEFPNEMSKIISNISKITEGHTLALFNS
                     KDRQEKTYEILKKYLHSFNLEIYADKKGIRHLNDLNRKCVVLGSKGCFEGVDIPGDGL
                     VCVTLDKLPNLNPKDPLYFTIMKKYGIDYYTINYPQMTIKVKQAMGRILRSKYDYGCF
                     VIFDVGTNISVLKRLEKDLHDCKISKVNSNEFYTYIRRHLNKSRSLILKSVIFDTIKA
                     LNVDAKMDNNDVDKDIIKKDINENIRQRAVKGEVYHIDIIKKDMKVKYFDRNYLINLD
                     IFMREEDKN"
     gene            9597..10196
                     /locus_tag="PC414_000999"
     CDS             9597..10196
                     /locus_tag="PC414_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HDF2463408.1"
                     /translation="MAKQKFYAVKKGKNIGVYNTWDECKKQVNGFSGAEYKSFSTFQE
                     AKEYIDGSEKLSFQEDKEFIEAYVDGSYEHSVKMYGSGVVILKNNEVIKTYSEKGKEK
                     TLVSMRNVAGEIEASKIAMQYCIDNNVQNLILYFDYEGIEKWCTGVWKTNKEGTIAYK
                     NFYDSIKNKLNVKFTKVKAHSGNKYNEEADKLAKKAIGV"
     gene            complement(10423..12552)
                     /locus_tag="PC414_001000"
     CDS             complement(10423..12552)
                     /locus_tag="PC414_001000"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HDF2463409.1"
                     /translation="MPNLPSLGSKAPDFKANTTNGPIRLSDYKGNWIVLFSHPGDFTP
                     VCTTEFLCFAKYYDEFKKRNTELIGLSVDSNSSHLAWMYNISLLTGVEIPFPIIEDRD
                     MRIAKLYGMISKPMSDTSTVRSVFIIDNNQILRTILYYPLTTGRNIPEILRIVDALQT
                     SDRDNIVTPANWFPGMPVILPYPKNYKELKNRVNSCNKKYSCMDWYLCFVPDNYNDEE
                     VSKKIDNTCSWKKEHTKNIENECNCEHEHHDYLNKALDCKQEHKTDIKDDCNHEKKHT
                     KNTNKVHNSKQDKFKDKSCDEMNFNYDKDESCDKINSSYNKEDSSYEDFYKHNYKNYD
                     YTSEKNTKKIAMKTLKDSKKLVRPQITDPYNPIVENANCPDINPIVAEYVLGNPTNVD
                     AQLLDAVIFAFAEIDQSGNLFIPYPRFLNQLLALKGEKPSLKVIVAIGGWGAEGFSDA
                     ALTPTSRYNFARQVNQMINEYALDGIDIDWEYPGSSASGITSRPQDRENFTLLLTAIR
                     DVIGDDKWLSVAGTGDRGYINSSAEIDKIAPIIDYFNLMSYDFTAGETGPNGRKHQAN
                     LFDSDLSLPGYSVDAMVRNLENAGMPSEKILLGIPFYGRLGATITRTYDELRRDYINK
                     NGYEYRFDNTAQVPYLVKDGDFAMSYDDALSIFLKTQYVLRNCLGGVFSWTSTYDQAN
                     ILARTMSIGINDPEVLKEELEGIYGQF"
     gene            12818..13066
                     /locus_tag="PC414_001001"
     CDS             12818..13066
                     /locus_tag="PC414_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463410.1"
                     /translation="MMICFAMTFALVLINSVMGVHIGLDYYFLMGSVLTVCLTIGIVL
                     CNARKIFDSKAIHKTRTKTVSNNKKQKSHSRDIKRKIS"
     gene            13232..14623
                     /locus_tag="PC414_001002"
     CDS             13232..14623
                     /locus_tag="PC414_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HDF2463411.1"
                     /translation="MNFRDLFEKAVDFIDEKRKSIVAISLSTLGVIALIIVFFLSSNE
                     FSVGNEANELLKIIEKRQYSIAVDYYTSIEKKFSDSKMERFNKSVSKKINKLLLNSGD
                     KYLDGDISQESFIGLINTVKELNKISIDVDDLLAQADRVREMYKEENTTYDIAINYIS
                     TVSILNGMGSNLDVYKQNIETIKESRDVYDSAVKDQKAYKYYEAIEKYNKVLKEDEKY
                     YSMAQNGKEQCIEEMYDYYISRAEEANTSGDYERALQYIEYLKDDYSDDEKVQSLEKK
                     YKKNLSLYTLTSDDIINVIAKKSGKDKANLSINSLPQMIKNNKYYYAEVYEYDKLINE
                     VLVSAKTKDLYSYKDGKKDYKVDYGDGYFRILEDGSYQFGITKDKAKFVLTNTLDEKE
                     NKYKKIEILDIEKADRYVKSKKSLEELFGKYKNIYYYAVVNKGLFRGKEVYAINIYNE
                     KIYAISENGLNEY"
     gene            14738..16207
                     /locus_tag="PC414_001003"
     CDS             14738..16207
                     /locus_tag="PC414_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HDF2463412.1"
                     /translation="MSNVNKKLVIAIGAVALVVIIFIGIMCMQFKGEKIAKNTYVNGV
                     NIGKLTKSQAKQELAKKYKLENVDFNYNDKSWKVKSKDLNLSTDLDKTVENAYNLNRK
                     SAFFGNLSKTISANFGKKSNLVVAINYDKNKLKAEMEKIAKEIDVDVKDATLDISGEK
                     VKVIPDSDGLKMDISKSMENFDNQTKKGNYKNELVVKATPAKVKKEQLANIDTNLGTY
                     STTFKTSQINRSINIKLATDNISNVLLMPGETFSFNKHTGKRSKENGYKSAPVIMEGE
                     MEEDYGGGVCQVSSTLYNSVLYAGLEIVNVKNHTIPSSYVPKGRDATVADSGIDFLFK
                     NNLKHPVYIKNYVSGNQIVCNIYGSAEDKQNITISTKLDGVSQTTMKRVNDPTMPKGK
                     EKVDKSGRNAYSVSTYRTFNDANGKKIKTEKIANSYYPKKEGIILVGTMEPKPEEKPN
                     TDENKNNQNTNNQNPNNQQKPETPPTDNKPNETQPQPQA"
     gene            16272..16865
                     /locus_tag="PC414_001004"
     CDS             16272..16865
                     /locus_tag="PC414_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HDF2463413.1"
                     /translation="MNLNNITNYVQSICENISNVLDVDVTLVTKDLTRIAGTGIFRGK
                     IGETISEKSVYSLVLKDGKSYVINKEIEENCNKCANRENCKELADICTPVKMGEHILG
                     ILGIAAFNEEQKEKILSKDKELTAFVRSMSDLISFKLDELYKQEVKTIDDLEREAIEN
                     AIKKYGSNTEGMKKVAEALNIGIATLYRKVKKFNIKS"
     gene            17047..18393
                     /locus_tag="PC414_001005"
     CDS             17047..18393
                     /locus_tag="PC414_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDF2463414.1"
                     /translation="MPRKILDISLIEDNKSDLTFFNIEEEDGDETLKALKQSYVTTNY
                     KEETLLNLQGALNSKSDTTSKELKLESYKRYNNALDLAYKNYITSAINMVNKALEINP
                     KDIDILNLRGLLKLLKCDFAKSFESFYTALCYENNHISRKYVNLISSEDFKTFLGRYN
                     HSIRFINEELNYESIHILENIIEEEPELIEPYVILSLLYDKLGNVKKREGYLDKLKEL
                     DKDNPVFEKNEEEKEDTSKKEEIKKVKRKKSILPYIIVGCVLIGMGIYLIQSKKRIEN
                     LNNQISSKEEKLSETDKKLGETSKKLEKTNKELNEVKNKEPEREITVADEEDLYYQAL
                     NLKKNKEYEKAIDNFKSVVSSGKTKKYISESIYQLAITNKLLGNKDEAIKYYKKYINT
                     YTKNDQYYDDSYYELGMLYYDNGDLKNAQQTFYSLRSEVPDSMYNNSKIKEILSEK"
     gene            18575..18946
                     /locus_tag="PC414_001006"
     CDS             18575..18946
                     /locus_tag="PC414_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HDF2463415.1"
                     /translation="MKVFNQLVIILSIWVIGEYISSFIQGIILIPGSIVGMLLLFLLL
                     QFKVLDLSSIENVSGFFLDNMAIFFIPAGVSLIKSLDLIRENVLVLLLVICLSTLIVM
                     YTTGIIVEKMIKKKEEGQKNV"
     gene            18939..19628
                     /locus_tag="PC414_001007"
     CDS             18939..19628
                     /locus_tag="PC414_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HDF2463416.1"
                     /translation="MYNVLQTPVFGIIVTIVFFNLGRYIQRKTSNPICNPLLIAIVGI
                     ILFLSISKIPYENYKIGADSLNFFLGPVTVVLAVPLYKQFELFKKHMFEIIVGIGCGI
                     VISFVSVLIIGKIANSDVSIINSLIPKSITTPMGISLANSLNGVESITVVAIIFTGIF
                     GGMVASTVFKLGNINHPVAKGIALGTSAHALGTTKAFELGEIEGAMSGVSIGVSGTIT
                     VILIPIIMNFI"
     gene            20148..21014
                     /locus_tag="PC414_001008"
     CDS             20148..21014
                     /locus_tag="PC414_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF2463417.1"
                     /translation="MSEVKNTLQKHPVLRYSTMLFGMLMTSIGINGFLRPAHLLSGGA
                     TGIATSINYLTNINVGLLTFLINIPIFILGFIYLEKEFCISSLVNMIVFSLLLGATQE
                     ISNIIPIHDILLQSVYGGILSGLGVGVVFRTRSSQGGTDIIAAILKIKKNIEMKDTAL
                     AINGLIVLTGSFLFGLDLALYTLIGLFLNAYSMSFIKDAMNYQKSVMVMSNEVDLIAE
                     DIMKSLVRGVTFLDAEGAYTHQKKKIIYTIVSSNEIPKIKDIALKYDKKAFISVNDVT
                     EVKGRGFKAKDL"
     gene            21256..22065
                     /locus_tag="PC414_001009"
     CDS             21256..22065
                     /locus_tag="PC414_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463418.1"
                     /translation="MNLSKEYVNLYDNYLKYINEVNKEIRSIKNMKKRVLKRGDFAPE
                     NEVLELEGKTIRDIDDVDTKKPKNKIYKFSNGDVYVGKFLEGKMEGKGSYTFFVDEGT
                     VMEYIGEFKEDKKNGKGNFIFSNGNEYIGYFEEDMMSGIGNMLYSSKDEYIGTWKNGK
                     KDGKGIYKWSDGCIYAGEFKGGKMEGYGICYNSKGEVLYEGEWKNNLIHGKGTYIWEK
                     GKKYIGEFMHGKKHGQGTFYLNNELVYEGTWKFDKPSIFDRTLDEIFSLRL"
     gene            22209..23273
                     /locus_tag="PC414_001010"
     CDS             22209..23273
                     /locus_tag="PC414_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDF2463419.1"
                     /translation="MKRLIALVLVVLIVLFYSPELLKEGYDLKEKFFQSEIWNEFLDK
                     ININDNSKKDKKEKSQNLQSNNTEGENLGESDIKNFEKISLGDNLSYVLSSIGKPGRI
                     DISEYGFDWYVYNQYGKEFAMVGLENDEVVALYSNSINSCENQDIKLNQDRQTVRTKI
                     TPLKYKRKGNTRYIINSENQYDIISKEGKYITIFYDIHEENRVCSYLIIDKSTEDEFK
                     NLYPDDSEELKKCFELEVIDLVNSVRNQRGLNSLRYSEQATLSSRKHSEDMRDNNFFD
                     HVNKKNETPFDRMKREGIVYTSAGENIAAGQINAIYAHEAWMNSEGHRKNILGNYNNI
                     GVGVIFGGSYKTYYTQNFYK"
     gene            23301..24020
                     /locus_tag="PC414_001011"
     CDS             23301..24020
                     /locus_tag="PC414_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2463420.1"
                     /translation="MTSWKKKTVYKCLIAVVLFCGIVLISNFSKVSALMMDTNGNVLI
                     KHGSREKKLIAITFDDGPHPKETSQVLDVLKKYNVKATFFIAGKHAKWYKEPLVRASK
                     EGHEIGNHTFNHPDISNLSSSQIEEEIVKCEDILKEVTGKKPTLFRPPFGSYREKELI
                     EIAKKHDYKVVLWTGVDVKDWKNPGANSIADKIINKVQNGDIILLHDYATNDTVEALD
                     MFIPKMIEKGFKFVTVSELIK"
     gene            24044..24628
                     /locus_tag="PC414_001012"
     CDS             24044..24628
                     /locus_tag="PC414_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HDF2463421.1"
                     /translation="MILMIDNYDSFVYNLVQYIEELGETVVVKRNNEIKISDIEELNP
                     EVIVLSPGPCSPKEAGICIDIVEHFKGKKPILGICLGHQTIGHVFGGDIIKAQQPVHG
                     KVYSINHTNKGVFRGLKNPLNVTRYHSLIVDSNTVPKELEITAITDKGEIMGIRHKKY
                     LIEGVQFHPEAILSEYGHEMLKNFITEARERVHM"
     gene            24630..25979
                     /gene="pabB"
                     /locus_tag="PC414_001013"
     CDS             24630..25979
                     /gene="pabB"
                     /locus_tag="PC414_001013"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HDF2463422.1"
                     /translation="MIREINTKLNSFEIFTIFRNEHDSFILDSAMDKEKLGRYSFISS
                     QPFKVLKYKDTDENPLEVLKEELHKYRVVNDTNLPFVGGAVGYLSYDLGNYIENLPRT
                     AVDDIEMPDMYFGFYNHVIVIDHLVQKTYIATPNIDIELEEKIIDDIEQRILKEEKKG
                     IDSICYEEKEVTPIRLKSNFTKEEFKNAVQSVREYIRQGDIYQANLTQRFSGETELTS
                     FELYRDLRRFSPAPFGAFLNFEDAHILSNSPERFIRCVNKRIETRPIKGTRPRGKDKE
                     EDLRLQQELRNSEKDRAELLMIVDLERNDIGRISKTGSVKVPELFVIEPYANVNHLVS
                     TVVGELKDDKDATDVIKATFPGGSITGAPKIRAMEIIDELEPTQRNVYTGSIGYIGFN
                     GDMDFNIAIRTIIKNDKKVYFQVGGGMTWDSDPDEEYQETLDKAKSIMKALRGYYEE"
     gene            25969..26712
                     /locus_tag="PC414_001014"
     CDS             25969..26712
                     /locus_tag="PC414_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HDF2463423.1"
                     /translation="MKNNVGFDSSLSKFGIGLFETIRVKNGIAVDLDIHIERMLSSIN
                     CLDLDIKYKKDFLINEIVTYIKKENVINKALRITVFDEGYNISIRDIPYNQETYEKGF
                     KLAISPIVRGNSLIHRHKTTNYFENIYTKNIANKTGYNDGIFLNSEGVILECSMSNIF
                     FVKGERVYTPSDRLPILNGIIKKRIIEICDELHIELIENEINISEISSFDFVFVTNSL
                     MGAIKVTEIDKIKFNKENVVFDKIVKLLE"
     gene            26746..27447
                     /locus_tag="PC414_001015"
     CDS             26746..27447
                     /locus_tag="PC414_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463424.1"
                     /translation="MIGCIYEVYNEEITKIIENQNTLAVYLVGSSKDVDFTLYDVEIN
                     DIDVFVFVKEGEKQERILFKKKGIEFDVNYFSKDGVKKLLSNREYFFVKEMKDAKVLF
                     DRENISHIIKDISRNIYLEGPSKMSLEEKSFIKQDIGAKISNLKNKEKFDVFEYHFLT
                     NLYLKDIIIGYFNINDKWVPKDKKLLKVLKKENNELFELVSKVSENYDYQRLLDVYNY
                     IFKEIETKEVIKLIF"
     gene            27473..28045
                     /gene="folE"
                     /locus_tag="PC414_001016"
     CDS             27473..28045
                     /gene="folE"
                     /locus_tag="PC414_001016"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HDF2463425.1"
                     /translation="MNKVDKEKIQHAVREILEAIGEDPDREGLIETPNRVARMYEEIF
                     SGLSEEPRDHLKVLFADEKHEELVLVKDIPFYSCCEHHLVPFFGKAHIAYLPKGGRLT
                     GLSKLARVIDTLAKRPQLQERITKNAADIIMEELQPYGVLVVVEAEHMCMTMRGVKKP
                     GSKTVTSAVRGIFEKDIASRAEAMSLITMK"
     gene            28109..28900
                     /gene="folP"
                     /locus_tag="PC414_001017"
     CDS             28109..28900
                     /gene="folP"
                     /locus_tag="PC414_001017"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HDF2463426.1"
                     /translation="MFDYGKRTYIMGILNVTPDSFSDGGDFNNLDIAIQHAKDMVDQG
                     ADIIDLGGESTRPGHSYVDSDEELRRVIPVIKKLKQELDIPISIDTYKADVAEEALKL
                     GVTMVNDVWGLRKDKNMASVIGKYDAEVCIMHNQDGTNYDKDIMESIKDFFKVSIEMA
                     MSCGVKKEKIVLDPGVGFGKDFEQNIEVLRRLNELKDLGYPILLGTSRKSVIGKVLPV
                     EPKKRLEGTIATTVLGIRDGVDIVRVHDVYENLMAARMTDAIYRK"
     gene            28911..29276
                     /gene="folB"
                     /locus_tag="PC414_001018"
     CDS             28911..29276
                     /gene="folB"
                     /locus_tag="PC414_001018"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HDF2463427.1"
                     /translation="MDKILLSNLGFYGYHGVLKEENFLGQKFFVDMELYIDSREAGLS
                     DDINKSVSYAEVYNVVKDITENKQFNLLEALAENIAEEVLNKFILINGVMVRVRKPEA
                     PVNGIYDYFGVEIRRTRDE"
     gene            29269..29775
                     /gene="folK"
                     /locus_tag="PC414_001019"
     CDS             29269..29775
                     /gene="folK"
                     /locus_tag="PC414_001019"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HDF2463428.1"
                     /translation="MNKAYLGIGTNMGDRFDNLSRACELLKNSDSIYKVKESSLYETK
                     PWGYTEQADFLNMCVEIETEFEPYELLEYCQEIERELHRERIVHWGPRTIDVDVLFFN
                     DVVSTDERLTIPHPRIQDRAFVLIPLMDLNEELIINEKTIKEHLNLLSAEEREEVKEL
                     VGYERKPI"
     gene            29756..30541
                     /gene="aroF"
                     /locus_tag="PC414_001020"
     CDS             29756..30541
                     /gene="aroF"
                     /locus_tag="PC414_001020"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HDF2463429.1"
                     /translation="MKENLFNNNSKIEINTNNKKITVKKDKLIIAGPCAIESYEQLLE
                     TAKFVKSQGANILRGGAYKPRTSPNSFQGLKKEGLEILKAVKDEVGMAVITELMDVRD
                     MDELYSISDIIQIGSRNMQNFTLLSEVGKQNKPVMLKRGIASTITEWIGASEYIAIEG
                     NSNIIMCERGIRTYNDYTRNTLDLAAVPIIQKETGLPVVVDPSHATGVRYLVKPMSLA
                     SFACGADGIMVEVHPDPENALSDGAQSLCFNEFEDLMKSINNY"
     gene            30796..32586
                     /locus_tag="PC414_001021"
     CDS             30796..32586
                     /locus_tag="PC414_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDF2463430.1"
                     /translation="MNDIKDIIEEIKARCDIASIISEYMNIKQSGANYKGLCPFHGEK
                     TPSFYINTSKQIYKCFGCGEGGDVINFVMKMENLDFMDAVKILANKCGIEINTNMNEE
                     TRIKIEKSKKFQDIHTEAARFYLSNLLGSKNLGYEYLRIRGLDDKIIKKFGLGFSLDS
                     WNSLMNTLISKGYKKQDLLECGLIAKNRDGTNCYDKFRNRVMFPIFDYRGNIIGFGGR
                     VLDDSLPKYLNSPDTLIFNKKQNLYGLNFARKNLESKTIVLVEGYMDLISLYQYGIKN
                     VVATLGTALTEQQGILIKRYADTAIISYDSDEAGIKATLRAIDILTKLGINVKVLDLK
                     DAKDPDEFVRKYGLSDYKKAMDVSTHYIKYKIDHLKKEFNIQKDEERVKFAKEASKII
                     KQLTSPVEIDFYTKYLSNQIDINVESIKREVYGKNYNKPYNNKNQKKIEEKVIEKVEV
                     RQDGKQLVEETLIKIMLEDKKIREIALLKVEESDFLLKESKEILNYMIKNQELDKITI
                     DKLKSLNISEEYLKELNSISLNSINLENTKEIEGIITNIRKNSLEEQINSLLREQQEL
                     ENNNDMKEVDGRVMEIALKIVEINKILKSL"
     gene            32604..33770
                     /gene="rpoD"
                     /locus_tag="PC414_001022"
     CDS             32604..33770
                     /gene="rpoD"
                     /locus_tag="PC414_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HDF2463431.1"
                     /translation="MENKSNKKELKKVTAKTLIEKGKKQGSLTLAEIMEAFSETELDK
                     DQVENLYETLGNLGIEITETKNYKADIDFSVADDDLSIGHLDEDAEAISHDDSSAIEI
                     ETVDLSLPKGISIDDPVRMYLKEIGKIPLLKPHEEVEFARRMHEGDEIAKQRLVEANL
                     RLVVSIAKRYVGRGMLFLDLIQEGNLGLIKAVEKFDYTKGYKFSTYATWWIRQAITRA
                     IADQARTIRIPVHMVETINKLIRVSRQLLQELGRDPKPEEIAKEMEMTEDKVREIMKI
                     AQDPVSLETPIGEEEDSHLGDFIPDDDAPAPAEAAAYSLLKEQIEDVLGSLNDREQKV
                     LKLRFGLEDGRARTLEEVGKEFDVTRERIRQIEAKALRKLRHPSRSKKLRDYLD"
     gene            33935..34624
                     /locus_tag="PC414_001023"
     CDS             33935..34624
                     /locus_tag="PC414_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HDF2463432.1"
                     /translation="MKLTDRLLKIASLVSDGKKIADIGTDHGYIPVYLLKEGKVPFAV
                     LADVNKGPLDNAHKEVIQNNLLDKVDLRLGSGIEILEIGEVEEVIIAGMGGILISELL
                     EAKKEVAHNVEKLILQPMQAQEELRYYLLNNGYEILEEVLVREDFRIYEIIVAKYTGK
                     NTIIEDEIYYEVGIKLLENKDSLFNDFIEKKIKTYSSIVNKLEGKNGEAIDKKRKESE
                     VAIKKLENLIK"
     gene            34638..35735
                     /locus_tag="PC414_001024"
     CDS             34638..35735
                     /locus_tag="PC414_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HDF2463433.1"
                     /translation="MLLKSLTRKIEKKYPLNLAEDWDNVGLIVGDFDMDVKKVLVSLE
                     ANEDVIDEAISKNIDLIVTHHPFIFGKINKINSGDLKGRLIQKLIKNDISLYSMHTNF
                     DIAFDGLNDYFMEIMEFGNSKVLDITKSENLYKLAVYVPHNYSDELRKVLSNSGAGHI
                     GNYSDCTFSTEGEGQFKPLEGSNPFLGSVNDIETVNEVKIETVVPQKLLGGVISSMLD
                     AHPYEEVAYDLYKLENKGEIFGLGRISKLDKSMTLESLSRKIKEKLNMKHIRVVGNLS
                     TDITKVAVVTGAGSEFVKKAKRQGAEVLITGDVKYHEAQDALDIGMCIVDCGHFDTED
                     IFKNVMKRFLDEFSEIEVIKSNVYLNPFSTI"
     gene            35798..36370
                     /locus_tag="PC414_001025"
     CDS             35798..36370
                     /locus_tag="PC414_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDF2463434.1"
                     /translation="MKVAVIFHSVCGSTYLLAREYKEALEEMNIEVDIFRVSDEVAKT
                     LPQYYLINSKEYKDEFESINVIKSGKEILDYDAIFMGSPTYYGNVSGPMKMFMDSFSD
                     IWVGAPLSGKIFGCFATAGSQHGGGELALQAMNIFAQHMGMTLLSVPCSVRGGYPAYG
                     ILHIAGDNSDIRPNDDAKIGIRDYLKRLNI"
     gene            complement(36430..36897)
                     /locus_tag="PC414_001026"
     CDS             complement(36430..36897)
                     /locus_tag="PC414_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HDF2463435.1"
                     /translation="MFKEMRLKKREMTKEDTVEVLKNGEFGTFSTISENGYPYGVAVN
                     YVYFNDSIYFHCARNGHKLDNISKNNKVSFLVVANESVIPDKFSTTYSSAIVFGKACT
                     VENEEKKNALVEIIKKYSKGFFEEGMKYIEKDMNLTTVVKIEIDHISGKASRL"
     gene            complement(37283..37759)
                     /locus_tag="PC414_001027"
     CDS             complement(37283..37759)
                     /locus_tag="PC414_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDF2463436.1"
                     /translation="MLSKAEIKYFNECASEILSSEKVQLMRTFPHHGNVSCLEHSLSV
                     AYYSYLLCKKLHLSVDIQSVIRGALLHDFFLYDWHYKGDRKGLHGFTHPREALKNATL
                     FFQINEKETDIILKHMWPLTVKPPRYKEAFIVCLLDKFCCLVETLKIHSLLSPYHV"
     gene            complement(37879..38970)
                     /locus_tag="PC414_001028"
     CDS             complement(37879..38970)
                     /locus_tag="PC414_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HDF2463437.1"
                     /translation="MSKIECLFTIFPVLILMYATFYFIPYFVGKKHIYGVSIDQEHKN
                     YNYFIKLDKEFKKLLSLGFIIDFILVFILVFTFNKLELSYFIGIIGFLLYESILYIHT
                     HKKAKKLKSEIYAKLGHIDIDSKLIVDMDFINKKNKIIKKFKIIYLIPILFTLGMSIF
                     IAFNYNQLPDSIATHWNINGSPDVFIDKSFLNVFKLIGTDFCLMVLLYITSIGSIKSR
                     IKIDTNRIEESRVENIKYLNKIGYLFLILMIIMTTQFFTTLLSIKTKLLTAMNITTLL
                     VIIYLIATYINSPNLKFNSSYSPEEDEKYWIAGIMYNNPNDPSLMVNKRFGIGWTINF
                     GNPLGKILYIAIALLLIFSLFSLIKSLLL"
     gene            complement(38963..39352)
                     /locus_tag="PC414_001029"
     CDS             complement(38963..39352)
                     /locus_tag="PC414_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2463438.1"
                     /translation="MVLNLDFNSDKSIYVQIKEEITKAIASGELKINESLPSVRSMAE
                     NIGVNLHTVNKSYNELKEEGFLNIDRRKGAIVAQLPMKIDNTTRQKNKLDLELLVAKS
                     YLSGISKEEFLSLSSSLFDKYEVKYYE"
     gene            39544..39990
                     /locus_tag="PC414_001030"
     CDS             39544..39990
                     /locus_tag="PC414_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463439.1"
                     /translation="MLTYLMKRQMHKKDDGMSKPMMITGAVITGVGAYAVYKMVKSHK
                     SNSQMVNTNYYGNSDYDDYKYDEFDYDCDCRDTKQDYEYEELKKKVKEFNSRRINCEN
                     CPHVSQEEMMQYVNSVKDVKEELDLHEDAKKDNIYKEDEYKKYEEE"
     gene            40254..40979
                     /locus_tag="PC414_001031"
     CDS             40254..40979
                     /locus_tag="PC414_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2463440.1"
                     /translation="MKRKEIVMLKLLIVEDDSTISFGIKYALEQEGFSIDISKDLSSG
                     KEMISSNEYSMILLDVTLPDGTGYELCQYIRGFSQVPIIFLTACDEEVNIVMGLDIGG
                     DDYITKPFRIRELISRIKAVLRRKGNTSEENKKILKFGDLSIYTLEARVYKNDKEIFL
                     TSVEYKLLLILIQNKNTVLSRTQILEKLWDVTYDFVNDNTLTVYIKRLREKIGDDLCE
                     PIYIETVRGIGYKWIGSENSVSI"
     gene            40966..42210
                     /locus_tag="PC414_001032"
     CDS             40966..42210
                     /locus_tag="PC414_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2463441.1"
                     /translation="MFLYNPEVKKFLSKYVTLMFIVIIISIGFSIINVSLTKEMVVRN
                     NQAIIGTLSSKYPNLESQIVDIITQGKSMENIDYGQKILIKYNYDKSIRINSEPIISK
                     LVLETIKINIILVCIIFILIFVLIVRYFTSIYNDLSDMTKYVYYSSEGKPFDMKNKNQ
                     EGQIGLLKTELLKMTTILNEKVELLKREKIFLNNTISDISHQLKTPMTSLIMLNDLLY
                     NDVPYEVKIDFLNKIKNQLNRMDWLIKSMLKLSKVEAKVINFKKDKVKFSELIHRAML
                     SMKIPMEIKNQKLTIEGNDNVSYIGDIDWSVEALVNIIKNCVEHTPEFGNIIITYKEN
                     PLFSELIIKDEGEGIDKKDIPHVFKRFYRGRSSSKEDSVGIGLAMSKSIIESQNGDIY
                     VNSEKGKGTEFHIIFHKMYDSD"
     gene            42290..42967
                     /locus_tag="PC414_001033"
     CDS             42290..42967
                     /locus_tag="PC414_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2463442.1"
                     /translation="MEILRVENLTKSYGKNETKVDAIKNVSLSVEKGTFIAITGPSGS
                     GKSTLLHLLGGVDRPTSGKVYINDVDIYNLKTKDLAIFRRRNIGLIYQFYNLIPVLNV
                     KENILLPAELDNRDIDREYFDDLMKTLGLIDREKHLPNQVSGGQQQRTSIGRALINRP
                     SIILADEPTGNLDSKNSKEILELLKLSVKKYNQTLIMITHDKNMALQADRIISIEDGI
                     IKSDEVM"
     gene            42968..45529
                     /locus_tag="PC414_001034"
     CDS             42968..45529
                     /locus_tag="PC414_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2463443.1"
                     /translation="MNLYTSLTIRYLKQNKRRTIVTIIGIILSTALICGIGNIFESFM
                     DYQMRETIKNDGSFHVTFYDVNRKNVEYVTKSAEIEKHAFTKQLGYSKLENSENAILS
                     IKQYDKNAINGYKVSIKEGRFPAKEGEIVLSESIINLIDDKLKIGDKITLKVGDMFDS
                     SKKKIDSMTFHEGDYIANEKDRTFKVVGIIEKPGFERYNGIATAIAYFNPMNNYNDTI
                     NVSVAVKNPKDVYKISNKISKNIYSNKDKEAVSDMIKYNEHLLRLQGASKYSNINSSI
                     KSIIIAVTILVIVCTIATVYNSFSISINERKKQFGILNSIGATSSQIKRLVFIEGIII
                     SLIGIPIGLISGTVAIDLLFKIINKYFTESVVTKMSLQIVYNPIIIIVSIIIVLLTIF
                     ISILLPAISASNISPLNVIKNTGEYKVGKIKSSRLIKMIFKTEGVLAYKNIRRNRKKF
                     IITLFSLMVSIIIFISFSGFTLLLLRGEEIRNSQRKYDLYLTAKNTARSVEDTISELE
                     DIDGIKNFELATGQYVSIRVSENKINKSKEDLIRKYYQKYKIGDSYEYDFSNNELIFP
                     GDFAVKNKINNLVQGSFNKERAIEENGVILVRKSAIEENGKKGVVELTNYKVGDTVNC
                     EYLDENALSKKVKVKILAITDEERLGLGYQNMGLQFITYDEVAKNLNLKLSKSLIFID
                     SGGSIKTKKEIEALANKNNFNFYDESASNESEKQDLKVIKIFVYGFIVVISLVSVTNI
                     LNTVSTSINLRKRELAVIQSIGVTPKGFRKMIYLESFIYGILSLLFGIPISIGIILIM
                     NKLISGVIEFSPIIPWTAIVICIVSVFIITFIAGYIPMSKLNKENIIDNIRRESI"
     gene            45563..46366
                     /locus_tag="PC414_001035"
     CDS             45563..46366
                     /locus_tag="PC414_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463444.1"
                     /translation="MEKLRVIEMIKNNPNIIATIDNPTDEMKLLAIKENGLVLEYINN
                     PTREMQELAIDNNIRAIKFIDNPTEDMMIKAVNEGWSILDYIKNPTDKVIEMAINQAG
                     WAIKYAKNPSEELQLLAVRKNYDSIKFIKQPYESVQEEAVRISYDALRYIDSPSYNAE
                     LIAIKNNEAAISFITDLDKNKMLEFLKVNILVIKYILKKVSKDELESALKEVLSKEDV
                     EEKYIRDFLNCSVIDRNSESIPIDKIMFIYKYGSKTAKKIAVDEKLKIM"
     gene            46378..47508
                     /locus_tag="PC414_001036"
     CDS             46378..47508
                     /locus_tag="PC414_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2463445.1"
                     /translation="MNFIDTLKSVDLILSTGEVPLIVGESGIGKTALANKLAKENDWS
                     LIVIDGNLLKEGEIGGLPTIESYVGVNSNGYKTEKKTTVYAVHNKLREIDEEISKSKT
                     VLLFIDEINRCEHTVQQELMNLILNREINGYKLHDDVKILAAMNPSSKYGSDFDYQVV
                     DMDAAQENRFVWLNMESDHTQWIKWAIDEGIERKVIEFISTFPEYLHKINEDDVRATP
                     RSYERVSKIYKVYKEKNNSIPRAVFLNVVKGNVGKVIAEEFISFIESNSEPLISYEDV
                     FLGESIDESIVERVKNESHTRLYLSAMNILKDLELNIKNDKYESNHYINRFIEFLKMY
                     PVDLMIGIMKDIRNSYIEVYKKAIENEEFVKSYFESYSLIRG"
     gene            47512..48921
                     /locus_tag="PC414_001037"
     CDS             47512..48921
                     /locus_tag="PC414_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDF2463446.1"
                     /translation="MENYFDKQAKELYNKASEIINTHSMLKANRANEKFDIDIPQDFK
                     YEFFSLVDKVNLSLMEEEDNFYGYFLFQMSREIRFDISSPTGVNFKGAKYIIYFNPII
                     FLTLDIKQMETTIKHEIHHILSMHLMRAKELKGKYSTLAINMAMDIVVNKFLNNLPPY
                     ATTLEWVNLKYSLKLEPYEPFEYYVEKIQTELDLMEVDEDGEEDDSDKYGDIEIDYSP
                     ERTHDIWDESNEIDENTLREFTEKFINSSQKGKVPAYLESMISSLKNSKGELPWNLYL
                     NRIMGTVENNKKKTITRRNRRQPNRLDLRGELRSHKSEIAVALDVSGSISDEEFKQAI
                     KEVLSIVKNYNHEITIIECDNEIRRVYKVRTEKDIKDRIRIGGGTRFSPVFEYANNKK
                     INLLVYFTDGKGENKLQVIPRGYKILWVISGRGDKISLKEPYGAVKKLTKVKTNDEIL
                     EINDVRNDGYSMNSQAPIL"
     gene            49132..49641
                     /locus_tag="PC414_001038"
     CDS             49132..49641
                     /locus_tag="PC414_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HDF2463447.1"
                     /translation="MVMFLFKIDDYIMYGMTGVCKVMDITNERFTNGIKKEYYVLSPI
                     YSNNTVIKIPVDNEKVPMRKLLSKINVLSLINDIPNMDTSWIDNEKLRSEQFKKILRG
                     GKCEELIKLVRSIDNNREYVKSIGKKTHQADDNIMKEAERLLNEEFATILDISPNEVS
                     SYISSHIPQ"
BASE COUNT        20517 a         5059 c         8509 g        16342 t
ORIGIN      
        1 taattattag gatgtttatt ttcctatcct tgttttaaag atttttttag tatattctaa
       61 tactatagta ttatagagta ataatgttga tttaaaataa aaataagatt tcggaggagt
      121 ttattatata aatgaaagat tactataaaa taggagagat atctaaaata tatggaatag
      181 gtagagattc attaatgtat tatgaagaaa taggcattct aagaccagtt agagatataa
      241 atggatatag aatgtataat atatcagata tatggaaatt aaatttaata aaagaattta
      301 gaagcttgaa ctttccaatg aagaaaataa aagaatacct agatgacaga agtatagagt
      361 ctacgaaaaa tattttaaat gaagaactcg atttaattga taaaaaaata gctgaattta
      421 taagccataa ggaaaatatt ataaaaagat tgagttcaat tgaaagtgta attcaaaata
      481 caaaaataga tgagatagag gttgtatata tagaaaaaag aaaagcttta gaattaaatg
      541 ctgatataaa gagggatgaa gacttcgatt ttttaattca aaaacttcag aaagaatatg
      601 aagatagatt taatatactt ggaaacaaca atattggttc tgccttttct actgaagcaa
      661 ttaataaagg catatttaat gagtttaaat ctgtattttg ttttctggaa agtaatgaga
      721 aagtatataa tatagttttt gatgaaggat attatgtaac tttaaattat actgggagta
      781 attctaataa taagatgtat atggagaagg tgtttaaatt tatagaagaa aataattata
      841 agataattgc tgaccctata gaaatctata agatagatat acatgagact ggaattgtag
      901 aggagtttgt aacagaaatt caagttccta taactaaata tctatatttc aatggattag
      961 atggtaaata gaatgaaaaa atgttgattt ggcaccgatt tgccactgtg ttataaaatc
     1021 ggtagcaaat tatattttaa attataagat agcatgaaaa aataattgtt tagggggtta
     1081 taaatgtgaa atgttctaat tgtggaagtg taaatattga aaagggaatt ttaacaactg
     1141 gtggcatata tgctgaaact attggattaa aatatagagg agatggaaaa ttagataaag
     1201 ttataaataa aacttttcca gtacaatcta ctatatattc agatttatgt ttagattgtg
     1261 gcgaaatagt tagaacctat ataaaggata atactgatag gaattggtgt aaagataaga
     1321 aatagaataa tttttgaagt tgaattttat taaagataca agtgaaaaag tataaatggg
     1381 aagaaattgt tatatggaag aaaactttgc atatatggaa gattagtttt ttaatataat
     1441 aacattttta aatgtagata aaactaaaaa gggttctcta gaaaacaatt tctatttaat
     1501 tatacttact taccaatacc atcacttgat gtaaacaaga ttctactaca ttttctccgt
     1561 actctagtat aaaatcatct agctgtgtat ctataaaatc acaataatca tataaactac
     1621 taatataagc agttaacatg tgtgtacatt tatccatttc atctctaaac atatatatta
     1681 ctatccccct aaaaaacttt cataaaacaa aaagaacaat atctttttgg caactggctg
     1741 acataactca tagtttattt tataagcgtt agtccctata gctttgcgtc actaaatttc
     1801 tctagttttg ccgatttagt tttattctac aactaaaata atacactagg ataagttatt
     1861 gttcaacata cttgtcagaa aattgaaata tttaacaata tcttaacaat atgaaatcta
     1921 tttttcactc aagaaacaaa ctatatgtat agaatgttta ataaaaatta tatttagttt
     1981 tgtattgaag tttttgtatt aaaaagttta gttcacaagg gttacttggc tagataatag
     2041 ataaacaaaa atatcttgaa aaaatatata tgaatggcaa aaaacaaggg atgaatttta
     2101 tatagactca tcccttaatt atttatatat tgaccttgat taaatatatt attattttta
     2161 agcttcaatt tttatattat atttctaaag tttctataag aggattatcg ctaaataatt
     2221 ttaataaatt tgttttttta tatcctttag gtaacttagt atatataact acttctaacc
     2281 aatccccatc aaggcgttta atattcatgt ttataatttc aatattagat tttttaaatt
     2341 taccttcaat atattctatg gaatcagtgc tgttagaaat tttaagagaa acttgctctg
     2401 ctgtagggag tttcatccat ttcctatctc tatgaagtgt tatctgtaca ataacaataa
     2461 tgatacttga aacaatacca actaagtata atccagagcc aatggcaaga cctgtaccgg
     2521 ctgttgccca tataccagca gctgtagtaa gaccacttac tgattgattt ctaataaata
     2581 ttaatccagc acctaaaaaa cctataccac tgacaacttg tgctgcaacg cgggctgggt
     2641 ctagaccaat acctggagta cccactatat cattaaatcc atacttggaa acaatgatta
     2701 taagtgctga agctaatgcc actataaaat gagtgcgtat tccagcttct tttagtctat
     2761 tctttctctc atatccaatt aaagccccac agaaaccagc agtaatgatt cttgctatat
     2821 attcaaattg aatcaatata tgatggttca tttatctagc acccccttct atataatatt
     2881 atatatacta taatactaca ttttttatgt gaatatagta aagtttaaaa tttatataat
     2941 agttatatat gaatataaaa tgtataaacg tatattatgt atataaaaat gatagaaagc
     3001 atctaatttt aaatgattaa agagcaagct taaataaatc aaacttgctc ttttaacata
     3061 gtgtatgttt ttatttgtaa tagttagata aaaatttatc tgtgtttttt acatctgcaa
     3121 aactgttctg tagtgatgcc atgaatactt gatgtacagt atttgcatta atttctatat
     3181 tattccaaga taaattcttc gttgtacatg catcgcttat tagagttatg tcgtatcgaa
     3241 gtttctttgc tgttcttaca cttgtatcaa tacacatatg gctcatcatt ccacagatta
     3301 ctagattagt aatatttttt ttctcaagga tactttgcaa atctgtttca aagaaactat
     3361 ctggtgtgtg tttaattata atttcttcat tatctagtgg aaaaacaatt ttatttattt
     3421 ctgaaccatg agtattagga agaaaaaaag aagctgtttt atctgttgat atatgctgta
     3481 tatgaataac tggtaaattg ttttttctaa aaaacagtag tattttttta gcattttcag
     3541 cagtttcttc tgattgaaat aattcacatt ttccattttt aaaataatca ttttgaatat
     3601 caatcaatat taaagctata gacatgatta tacctcctat ttatttttat tataatacta
     3661 ttgtaaaaaa tgtaaattac ttactttttg gtatgtatag gattacttat tgtagcaaat
     3721 tcctttatta tctagaattt cttgttgacc atgttcaagc atatattcga ttcctatttc
     3781 ttgcattatt tccactgctt ttaacatttt tttaccacga ttagtaagaa aatattcaac
     3841 atgtaatgga tatccagcag aacttatttt atcaataatc ccaaaatcac gtaactcttt
     3901 taattgttga atcaacattt tttgatttat tccataaata ttatgttgca attcagaaaa
     3961 tgttttatta gattgtctta attcaaatat aaggatagtt ttccattttc ctttgattat
     4021 gtcatgtaca atttcaagag gacatgtata ttcagctctt attttcatag catatcaccc
     4081 ccagtaaaat gataataaat tatatcactt gtcttgtgat atgacaaata tatatgaact
     4141 gtatataaat tttatgtttt attaagttct aaatttcttc tattgtagtt catatttagt
     4201 acataaataa ttagtaagat ataagtaaat aaatatataa catgactata gaaatttaac
     4261 tttaaaataa cttatgataa tttagaggag gaatatatat gaatatatca agaaaagcta
     4321 tgaaaataat agaattagca caaaaaattg caaataagag aggtatttca gtagaagaag
     4381 cttggagtga agcagtaaca gaatataaaa ataaatacga acatattgca tagatattta
     4441 aaaatttaga atatttggta gaatataatg attttaaatg atactataaa ttataaggta
     4501 tatcattatt aacatagaag gaggatatct gcttgattgt aaaaatagat aatactttag
     4561 aaaaagaatt ttggcaatat gtatcacatg aagaaagttt aaatttattc ataattggat
     4621 acgttgagaa ttatggattt agctctcaat atcaagatat atggtctcag gtagaaggtg
     4681 gaaatataac ctctataata ttaaaaaata agtcaacact tataatttat agttttaaaa
     4741 ataattttaa tataggagaa atgaagaatc atataaaaga tttagatgta gaaagcataa
     4801 gtggaaaaaa atgtgtaata gatagactaa taagtaaata caaagatttt tatgaaaaac
     4861 tagataataa attttgtgta ttaaaagaaa taaaagaaat agatttttcc aacatgaagg
     4921 agtataaaat agaaaatgct caggaaaaag atattgatga aataggaaaa cttttaaata
     4981 ggtcagacta taaagtaagt aaaaattaca tagaagaaag aaaagttcat ttaaaagagg
     5041 gaaatgtaag agcatatttt attagaaatg atgatactat gattagcact gtgtcaactg
     5101 gtatggaaac tagttttttg gcaatggtgg tctctgtaag tacagataaa agatataggg
     5161 gaaaaggact tgcaagttat atggtttaca atttgagtaa agaattgttg ttagaaggaa
     5221 aagttccatg tcttttttat aataatgatg tagcaggtaa aatatatcat aatataggat
     5281 acaaagagat aaatgaatgg acaattcttt ttaaatagct ataaaaaata aaaaactcta
     5341 tcgagaatta aagtatttat aaataactat atttttacta taattcatga tagagttttt
     5401 ttaaatttat gatactattt tttattattt aaataatcaa gtgttttaag tttataacct
     5461 tctttttccc aatgtgtaag aacctcatct aatatctcag tatttgttga tgaatttgga
     5521 tgcagtaaaa ctattgcacc aggatgagtt cttgaataaa ttttgttttt ggcaaattca
     5581 tgagtaggtt gtttcttttc ataccaatct acataagcaa aactccaaaa tattgattta
     5641 tatcctaaat cttgtgtata ttttaaagat tgttcgctaa atttacccat tggaggtcta
     5701 aaatacttag gcatttcttt tccagtaaca tctttgtatg ctttttctac acttgtaatt
     5761 tcttctttga atttttcaaa atctgtaata cctgccattg atggatggct ttttgaatga
     5821 ttacaaacta aatgtccttc tttttccata cgctttatta actctggatt gctttttata
     5881 taactttcaa ctacgaaaaa ttgagcttta gcattgtgtt ttttaagcgt atctaataat
     5941 tttggagtgt ttccactttc atatccagca tcaaatgata gatatataac tttttcattg
     6001 ggatttccta cataataagc tccgtatttt ttaaaaaaat cagcttcttt tataggtgat
     6061 ggctgtttac catcttccct agggttaaaa taccaatcat attcatgtgt gtcgagtgtt
     6121 ggactagaaa tttgagtttt tgttttatct aatgatttaa aatttatact agcaattcca
     6181 aaaagtgtta gagcaaatgc tcctatcata atgtattttt ttaggctatc ttttttcaca
     6241 atttcacctc ttatttaaaa tttaaagtat atttcttata atatagtttg aatatttata
     6301 gttaaaaata ttcacatata caaataataa atgaaagaag tttactgtaa gatataaagt
     6361 tgatattaat tatcttaaat aaagtgtata taaatgataa aatataaaga gaaatgattg
     6421 taaataagtt aggctggttt aacttgaagg gatataaaaa tggataatat aataagtgta
     6481 ttgaatgatg ttgttttttt agatatagaa gttagtggat tagattgttt aaattcagaa
     6541 attttagaag ttggagctgt caaggttaaa gactggaaaa tatataccta tgaatcatta
     6601 ataaaaaaca aatttgaggt acctatagaa gtattctcag tatgtaaaaa tttagataaa
     6661 aatgatttag aaatagctaa taaaatagaa ctggtggaag atagacttgt aaattttgta
     6721 gaagatagtt tcattatatg tcatgattta agtctgaaaa aaaagttttt tgagtatcat
     6781 atgccaaagt taaaaaataa atttattgat ttaattgaat tagcagttat tttagaacca
     6841 tatcataaag attatagctt agaatactta aaaaatactt taacaaattg taattctaaa
     6901 gtggaaaata gagctttatc agatgctatt gatataataa atattgtaaa ttgcctttta
     6961 gtaaaattta ataactatga aaaaactact ttggagccat tgagttttaa aataaattca
     7021 tatcttaaaa aatttaatct tccaacatgg gaatggagca aatttttaga agaggcaaat
     7081 tatgatttat caaataatat aaatataaaa aaagaatata atatatttga ctcaaaagaa
     7141 gaaaagaaga aggaaagaga aaccttaaaa attttaaatg aagaagaaaa aaattatgaa
     7201 gaattattaa aatacaaaac tatttgggag aataaagaag gatttacata tgaatataga
     7261 cctggtcaat atgagcttac taaaacaata agagagttgt ttagaaatag cgaagatgaa
     7321 gaaaaaattg catgtataga ggcaccaact ggtataggta aaagtgtagg atatttattg
     7381 cctgcaatat tagaggctag aattaataaa aaaagattat taatttctac tgacacaaag
     7441 gaattgcaga tacaattaat aaataaagat attccaaatg tgttagattc tttgggtcta
     7501 aatggaaaag taagttacgg atatataaag ggaaaaaata actatatttg tatagataga
     7561 ctggaagcat atatagatga ttatgagtct caaaacccta ctaaaggaga aattttatct
     7621 ttaatatttt tgaagagatt agttgaaggt ggcaagtatg gagatataga agaaataaat
     7681 tatttagtgt ttgacaactt caaggaaata agtactcact taagaaatgt aagttgtgac
     7741 ccaaatatgt gcagacctaa aaaatgcaaa aaagattgtt tatataaaaa cagaatagaa
     7801 gaattaaaag aagaacatat aacagtagta aatcattcac tattagctaa atggccgtat
     7861 aaggatgaaa aacctttaga aaatataata gtagatgaag cacataattt gactgaaaaa
     7921 ggatatgatt tcttttcaag tattataaat tcaaagtctt taaggtactt attacaagaa
     7981 atttatcctt acgagtttat tcaaaatagt agctttatat ataaaaaata tagtagaaat
     8041 atgagaaaaa taaaggcttt tgataaattt tataatgtat taaaaattgg aagagaagat
     8101 aaacaaaaaa ttgctagaag tataaatttc attattgaag agatagattc aatattaaag
     8161 tttggtaatt gtaatgaata taataatgta agtaattata atttaagatg ggaactgaat
     8221 ttgcaaatag atgaaattgt gggaaaacta aaaaaagatg gtatagatac tgaaataagt
     8281 tatagagcct atagtgaaaa gataaagttg agttgtgaga aaataataaa aaatttagtt
     8341 tcaataataa taataattta taggaatatt gatgatgata gtatagataa agaagcagat
     8401 atatataaat ttggaaaggc aaagacaaga gatttagagg atataaagat tatatttgaa
     8461 atctttttag agtatgatga aaaggatgat tatgcaagga tagttgaaat tgataagaat
     8521 tataatgact ttgaatttcg agtagtacca ttaaaaatag cagatttatt tgaagaaaat
     8581 attttatctc aattagaaaa aggaatattt ttatcagcaa ctttgagttt atctgaaagt
     8641 atgtcttatt ttaaaaatac attaggaata gacagagtta agaatgtgga aaagattata
     8701 gaaccaattt ttgattataa aaatagagtc tctgtagttg gattttctga tatttgtgag
     8761 tacagaaatt ctgaatttcc aaatgagatg agtaaaatta tatctaatat atcaaagatt
     8821 acagaaggtc atacattagc tctttttaat agtaaagata gacaagaaaa aacttatgaa
     8881 atattgaaaa aatacttaca tagttttaac ttagaaattt atgctgataa aaagggcatt
     8941 agacacctta acgatttaaa tagaaaatgt gtagtacttg gctcaaaagg atgttttgaa
     9001 ggcgtagaca ttccagggga tggtttggtt tgtgtaactt tagacaagct tccgaatcta
     9061 aatcctaaag accctttgta ttttactata atgaagaagt atggtataga ctactatact
     9121 ataaattatc ctcaaatgac cataaaggta aagcaagcta tggggagaat tttaagaagc
     9181 aaatatgact atggatgttt tgtgattttt gatgtaggaa ctaatataag tgtgttaaag
     9241 agattagaga aagatttaca cgattgtaaa atttcaaagg ttaatagcaa tgagttttat
     9301 acttatataa ggcgacattt aaataaatct agaagtttaa tactaaaatc tgttatattt
     9361 gatactataa aagcgttgaa tgttgatgct aaaatggata acaatgatgt tgataaggat
     9421 attataaaaa aagatataaa tgaaaatata agacaaagag ctgtaaaagg agaagtgtac
     9481 catattgata ttataaaaaa agatatgaaa gtaaaatatt ttgatagaaa ctatcttata
     9541 aatttagata tatttatgag agaagaagat aaaaattaag agtgggagtg atatatttgg
     9601 caaaacaaaa gttttatgct gtgaaaaaag gtaaaaatat aggtgtatat aatacatggg
     9661 atgagtgcaa aaaacaagta aatggattct caggagctga atataagagt ttttctactt
     9721 ttcaagaagc taaagaatat attgatgggt cagaaaaatt gagttttcaa gaagataagg
     9781 agtttataga agcatatgta gatggaagtt atgaacattc agtaaaaatg tatggttcgg
     9841 gtgttgtgat tttaaaaaat aatgaagtta taaaaactta tagtgaaaaa ggaaaagaga
     9901 aaactttagt aagtatgaga aatgtagctg gagagataga ggcttctaaa attgcaatgc
     9961 agtactgtat tgacaataat gttcaaaact tgattttata ctttgactat gaagggattg
    10021 aaaagtggtg tactggagtt tggaaaacaa ataaagaagg aactatagca tataaaaatt
    10081 tctatgacag tattaagaat aagcttaatg taaaatttac aaaggttaaa gctcattctg
    10141 gaaataagta taatgaggaa gctgataaat tagctaaaaa agctattggt gtataataaa
    10201 aaaatattta tacataatta catggtataa gagtatatct aaattcctac tttataatta
    10261 aacaacttgt aattaaataa attttgatta aaaaagcaga ttgtattatg aaaaaataag
    10321 tatctaattt ataaacaatt aataatagta ttaattagat acaacttatt taatataata
    10381 aaatctgctt tttatataac tataaaattt tactacattc tattagaatt gcccataaat
    10441 accttcaagt tcttctttta atacttcagg gtcatttata ccaatagaca tggttctagc
    10501 cagtatattt gcttggtcat aagttgatgt ccatgagaat acaccaccta gacaatttct
    10561 aagaacatat tgagttttta agaatattga taaagcatca tcatatgaca ttgcaaaatc
    10621 tccatcctta actaaatacg gaacttgagc agtattatca aatctatatt catatccatt
    10681 tttatttata taatcccttc taagctcatc ataagttctt gttatagtag cacctaatct
    10741 tccataaaat ggtataccaa gaaggatttt ttcagaaggc attccagcat tctcaagatt
    10801 tcttaccatt gcatcaacac tatatcctgg caaagataag tctgaatcaa aaagatttgc
    10861 ttgatgtttt ctaccatttg ggcctgtttc acctgctgta aaatcataac tcataagatt
    10921 aaaataatct attataggag ctattttatc tatttcagca cttgaattta tatatcctct
    10981 atctcctgtt ccagctacac taagccattt atcatctcct ataacatctc ttatggcagt
    11041 tagtaagagt gtaaagtttt ctctatcttg aggtcttgat gttattccag atgcactact
    11101 tccaggatat tcccagtcta tatctattcc atctaaagca tattcattta tcatctgatt
    11161 gacctgtctt gcaaaattat atctagatgt aggtgttaat gctgcatcag agaatccttc
    11221 agctccccaa cctccaatag ctacaattac ttttaagcta ggtttttcac ctttaagagc
    11281 aagtaattga tttaaaaatc taggataagg aataaacaaa tttccagact ggtctatctc
    11341 agcaaaagca aatataactg catctaatag ttgagcatct acattagttg gatttccaag
    11401 aacatattct gctacaattg gatttatatc tggacagttt gcattttcaa ctattggatt
    11461 gtatgggtct gttatttgtg gtctaactaa ttttttagaa tcttttaaag ttttcatagc
    11521 tattttttta gtattttttt cgctagtata atcgtagttt ttataattat gtttatagaa
    11581 atcttcataa ctactatctt ctttgttata gctagaattt attttgtcac aagattcatc
    11641 tttatcatag ttaaaattca tttcatcaca agacttatct ttaaacttat cttgtttgga
    11701 gttgtgaact ttgttagtat ttttagtatg ttttttctca tgattgcaat catctttaat
    11761 atcagtcttg tgttcttgtt tacagtcaag agctttgttc aggtagtcat gatgttcatg
    11821 ttcacagtta cattcatttt caatgttttt agtatgttct tttttccagc tacaagtatt
    11881 atcaattttc ttgctcactt cttcatcatt ataattatct ggtacaaaac ataagtacca
    11941 gtccatacat gaatatttct tattacaact gttaactcta ttttttaatt ccttatagtt
    12001 tttaggataa ggtaaaatca ctggcattcc aggaaaccag tttgcaggag taactatatt
    12061 atctctatca ctagtctgaa gtgcatctac tattctaagt atttctggaa tatttcttcc
    12121 tgtagttagt ggataataaa gaatcgttct tagaatttga ttattatcta taataaatac
    12181 agagcgaaca gttgatgtat cactcattgg ttttgatatc atgccgtata acttggcaat
    12241 tctcatatct ctatcttcta taataggaaa tggaatttct acacctgtaa gtaaagaaat
    12301 attatacatc caagctaaat gtgaactgtt actatcaaca cttagaccaa ttagttctgt
    12361 atttcttttt ttaaattcgt cataatattt agcaaaacat aaaaattctg tagtacaaac
    12421 tggtgtaaaa tcaccaggat gtgaaaataa aacaatccaa ttacccttat agtcagagag
    12481 tctaatagga ccatttgttg tattggcttt aaaatcagga gcctttgacc ctaaacttgg
    12541 caaatttggc atgtaaatca cccctcaata aattatcatt tattttattc tattaattaa
    12601 tacatattag tgttactatt ttattaaaag ttaagtaatc cttatgtcaa aaaaagatat
    12661 ttattaaaaa gatttaatat tttcctaata taaatgacaa tagtaattgc tattttttta
    12721 ctaatttggt atagtaatgg ataaggggta gtgtcaattt tagtgattat acgaggtgag
    12781 tggattgaaa aaaattctaa gaattttagt tatctccatg atgatatgtt ttgcaatgac
    12841 ttttgcatta gtgttaataa attcagttat gggagtacat ataggattgg attattactt
    12901 tttgatggga agtgtgctta cagtttgttt aacaattgga attgttttat gcaatgctag
    12961 aaaaatattt gatagcaaag ctatacacaa gacaagaacg aaaactgtca gtaataataa
    13021 aaaacaaaag agtcattcta gggatataaa aagaaagata tcataattta tgtaaaggaa
    13081 atatttatgt ataaaataat gattgatagg taaaataaaa acacgattta acttgtgttt
    13141 ttattttttt tcaagaatta tgatataata accaataaga aatttagttt accataaaat
    13201 ataaagatta ttaaatggag agggaactat aatgaatttt agagatttat ttgagaaggc
    13261 tgtagatttt attgatgaaa aaaggaaatc tatagttgcg atttcattat caacattagg
    13321 tgtaattgct ttgataatag tatttttctt aagtagtaac gagtttagtg taggaaatga
    13381 agctaatgag ttgttaaaga ttatagagaa aagacagtat tctatagctg ttgattacta
    13441 cacaagtata gaaaaaaagt tttctgattc aaaaatggaa aggtttaata agtctgtttc
    13501 taaaaaaata aataaattgc tattaaatag tggtgataaa tatttagatg gagacataag
    13561 tcaagaaagt tttattggat taataaatac cgtgaaagaa ttaaacaaaa taagtataga
    13621 tgtggatgac ttattagctc aagctgatag agttagagaa atgtataaag aagaaaatac
    13681 tacttatgat attgccatta actacataag tactgtttca atattaaatg gtatgggtag
    13741 taacctagat gtatacaaac agaatataga aacaatcaaa gagtctagag atgtttatga
    13801 ctcagcagta aaagaccaaa aagcatataa gtattatgaa gctattgaaa agtataataa
    13861 agtattaaaa gaagatgaaa agtattacag tatggctcaa aatgggaaag aacaatgtat
    13921 tgaagaaatg tatgattact atataagtag agctgaagag gcaaatactt ctggagatta
    13981 tgaaagagca ttacaatata ttgagtattt aaaagacgat tattcagatg atgaaaaggt
    14041 acagagttta gaaaaaaaat ataaaaagaa tttatcattg tatactttaa catctgatga
    14101 cattataaat gtaatagcta aaaaaagtgg caaagacaag gcaaatctat ctataaattc
    14161 acttccacaa atgataaaaa ataataaata ctattatgct gaagtatatg agtatgataa
    14221 attaataaat gaagttttgg taagtgcaaa aactaaagat ttatattcat ataaagatgg
    14281 taaaaaagat tacaaagtag attatggtga tggatacttt agaatattag aagatggaag
    14341 ttatcaattt ggtattacaa aagataaagc taagtttgtg ttaactaata ctcttgatga
    14401 aaaagaaaat aagtataaaa agattgagat attagatata gagaaagcag atagatatgt
    14461 aaaaagtaaa aagagtttag aagaattgtt tggaaaatat aaaaatattt attattatgc
    14521 tgtagtaaat aaaggcttat ttagaggcaa agaagtttat gctattaata tttacaatga
    14581 aaaaatatat gccatatcag aaaatggact aaatgaatat taattaataa tattaaatta
    14641 atatataatt aaaaagtttt tggggtttta aggcgcatta tgtagtaata taataatagg
    14701 gatatataaa taattttatt gtaaggagaa gtatgggatg agtaatgtga acaaaaagct
    14761 agttatagct attggagctg tagcattagt agtaataatt tttataggca taatgtgcat
    14821 gcaatttaaa ggggagaaaa tagcaaaaaa cacatatgtt aatggagtaa atattggaaa
    14881 attaactaaa tcacaagcaa aacaggaatt agctaaaaaa tacaaattag agaatgttga
    14941 ttttaattac aatgataaaa gctggaaagt aaagagcaaa gatttaaatt tatctactga
    15001 cctggataaa acagtagaaa acgcatataa tttaaataga aagagtgctt tctttggcaa
    15061 tctatcaaaa actatttcag caaattttgg caaaaaaagc aacttagtag ttgcgataaa
    15121 ttatgataaa aataaattaa aagcagaaat ggaaaagata gctaaagaaa tagatgtaga
    15181 tgtaaaagat gctacacttg atataagtgg tgaaaaagtc aaggttatac cagattctga
    15241 tggtcttaaa atggatattt ctaagagtat ggagaatttt gataatcaaa ctaaaaaagg
    15301 taactataaa aatgagttag tagtaaaagc tactccagca aaagtaaaaa aagagcaact
    15361 tgctaatata gatacaaatt taggtacgta ttcaacaaca tttaaaactt ctcaaataaa
    15421 tagaagtata aacataaaat tagcaacaga taatataagt aatgtattac ttatgccagg
    15481 agaaactttc tcatttaata aacatacagg gaaaagaagt aaggaaaatg gatataaaag
    15541 tgcacctgta ataatggaag gtgagatgga agaagattat ggtggaggag tttgtcaagt
    15601 ttcatctacg ttatacaatt ctgtgctata cgcaggttta gaaattgtta atgtaaaaaa
    15661 tcatactata ccatcaagtt atgttccaaa aggaagagat gcaactgttg cagatagtgg
    15721 aatagatttc ttatttaaaa ataatctaaa gcatccagtt tacataaaaa attacgtttc
    15781 aggaaatcaa attgtttgca atatatatgg aagtgcagaa gataaacaaa atataacaat
    15841 atcaacaaaa ttagatggcg tttcacaaac tacaatgaaa agagttaatg accctacaat
    15901 gcctaaaggt aaagaaaaag tagacaaaag tggaagaaat gcatattctg tatcaacata
    15961 tagaacattt aatgatgcaa atggtaaaaa aattaaaact gaaaaaatag ctaattcata
    16021 ttatcctaag aaggaaggta ttatattagt tgggactatg gaaccaaaac cagaggaaaa
    16081 accaaataca gacgaaaata agaataatca aaatacaaac aatcaaaacc caaataatca
    16141 acaaaaacca gaaacaccac caactgataa taagcctaat gagacacaac cacaaccgca
    16201 agcttaaagt tctcaagaat aggatttatg tagagtaata aaaaatataa atctaaatag
    16261 gagggattac tttgaattta aataatataa cgaattacgt tcaaagtatt tgcgagaata
    16321 tttctaatgt cttggatgta gatgttactt tagttacaaa agatttaact aggatagcag
    16381 gtacaggaat attcagaggt aaaataggag agacaatatc agagaagtca gtgtactctc
    16441 ttgttttaaa agatgggaaa tcctatgtta taaataaaga aatagaagag aactgtaata
    16501 aatgtgcaaa tagagaaaat tgtaaagagt tagctgatat atgtactccc gttaaaatgg
    16561 gagagcatat acttggaatc ttgggtatag ctgcgtttaa tgaagaacaa aaagaaaaga
    16621 tactttcaaa agataaggag ttaactgctt ttgtaaggag tatgtctgat ttaatatctt
    16681 ttaaactaga tgaactttat aaacaagaag ttaagactat agatgacctt gagagggaag
    16741 ctatagaaaa tgccataaag aaatatggaa gtaatacaga aggtatgaaa aaagtagctg
    16801 aggcattaaa tataggtata gctacactct atagaaaagt aaaaaaattc aacattaaaa
    16861 gttaatagta ttgaaaaaga ggtaaccttt ttatgaaaag aagaggatta ccttttttat
    16921 tatgagaatg gtggaaaaat atgtatagtt ttgacttgac atattaaata tgttatctta
    16981 aatatatatt ttagagtgga ggtgagtatt attttagtta aagatataaa acaagacata
    17041 gaaagcatgc ctagaaaaat tttagatata tctttgatag aagataataa aagtgatttg
    17101 acttttttta atatagaaga agaagacggt gatgaaacat taaaagcatt aaaacagagt
    17161 tatgtgacta caaattataa agaagaaact ctattaaact tgcaaggagc tttaaattca
    17221 aagtcagata caacgtctaa ggagttaaaa ttagaatcat ataaaagata taacaatgca
    17281 ttggatttag catataaaaa ttatataact agtgccatta atatggttaa taaagctctg
    17341 gagataaatc ctaaagacat tgatatacta aatcttagag gtcttttaaa attattaaaa
    17401 tgtgattttg ctaaatcatt tgagagtttt tatactgctt tatgttatga aaataatcat
    17461 atatctagaa aatatgtaaa tctaatttca tctgaagatt ttaaaacttt tttaggaaga
    17521 tacaatcatt ctataagatt tataaatgaa gaattaaatt atgagtcaat acatatttta
    17581 gagaacataa ttgaagaaga acctgaacta atagagccat atgtaatttt atctttgtta
    17641 tatgataaac taggtaatgt aaaaaagaga gaaggatatt tagacaaact taaagagcta
    17701 gataaagata atcctgtatt tgaaaaaaat gaagaagaaa aagaagatac aagtaaaaag
    17761 gaagaaataa aaaaagtaaa aagaaagaaa agtattttgc catatattat agttggctgt
    17821 gttttaatag gtatggggat atatttgatt caaagcaaaa agagaataga aaacttgaat
    17881 aaccaaataa gtagtaaaga ggaaaagtta agtgaaactg ataaaaagtt aggagaaacg
    17941 agtaaaaaat tagaaaaaac gaataaggaa ttaaatgagg ttaaaaataa agaaccagaa
    18001 agagagataa ctgtagctga tgaggaagat ttatattatc aagctctaaa tttaaagaaa
    18061 aacaaagaat atgaaaaagc catagataat tttaaaagtg tagtttcttc tggtaaaact
    18121 aaaaaatata tttctgagtc aatatatcaa ttagcaataa caaataaatt attaggaaat
    18181 aaggatgaag ctataaaata ttacaaaaaa tatataaata cttatactaa aaatgaccag
    18241 tattatgatg attcatatta cgagttgggt atgctttatt atgataatgg cgatttaaaa
    18301 aatgcacaac agacttttta tagtttgaga agtgaagttc cagacagtat gtataacaat
    18361 tcaaaaataa aagaaatttt aagtgaaaaa taggtttata tagttaagtt ttttgaaaca
    18421 accaagtatg tattttatta aattaaaata aaaataaatt taaaaagaga ttgtgtcaaa
    18481 ttataacttt ctattttaaa gaaagatatt tgcttgttga taaaactaca aatatatgat
    18541 ttgaagcaat ctctttaatt tttggaggtg gaaaatgaaa gttttcaatc aattggtaat
    18601 aattttatct atctgggtaa taggagagta tataagttcc tttatacaag gaattatact
    18661 tattcctggg agtatagttg gtatgctatt attatttcta ctactacaat ttaaagtttt
    18721 agacttgagt agtatagaaa atgtaagtgg atttttcctc gataatatgg ctatattttt
    18781 tattccagca ggtgtatcac ttataaagtc attagaccta ataagggaaa atgtattagt
    18841 gcttttatta gtcatatgtt taagtacact aatagtaatg tatactactg gaattattgt
    18901 ggagaaaatg ataaaaaaga aggaagaagg acaaaaaaat gtataatgta ttacagacac
    18961 cagtatttgg tataatagta acaatagttt ttttcaattt aggaagatac attcaacgaa
    19021 aaacaagtaa tcctatatgt aaccctttat taattgcaat cgtaggaata atattgtttt
    19081 taagcatatc taaaatccct tatgagaatt acaagatagg agctgatagt ctaaatttct
    19141 ttttaggtcc tgtcacagta gtacttgcag ttccacttta taaacaattt gaattattta
    19201 aaaagcatat gtttgagatt attgtaggaa taggttgtgg tatagtgatt tcttttgtat
    19261 ctgtattgat aattggcaaa atagctaatt cagatgtaag tataataaat tcactaattc
    19321 caaaatctat aacaacacca atgggaatat ctttagcaaa ttctttaaat ggtgtagaat
    19381 caataacagt tgtagccata atttttacag gtatttttgg tggtatggtt gcttctacag
    19441 tattcaaact ggggaatata aatcatccag ttgctaaagg aattgctctt ggaacatctg
    19501 cacatgcttt aggaactaca aaggcttttg aacttggaga aatagaagga gctatgagtg
    19561 gtgtttctat tggagtctct ggtacaatta cagtaatact aattccaatt ataatgaatt
    19621 ttatataaaa aataaaatta agacgagtaa cttgaatact taaaacaaaa taaaataaaa
    19681 aaaatttaaa ataaaaactg aacgaaaaaa atgcaaataa tgaaataaaa aaatcatgta
    19741 ctgaaattat aaaaagaaaa atttcattta ggaattaatt ataaagagac aattactaaa
    19801 cttgaatttg ctaaaataaa ataaattact gcgaaaatag atatttagaa aaaaatacta
    19861 taaatttact atattgcaag ttaaataata agcagtatca tattatgcta gaaaaagttt
    19921 catttaaggc ataaatgaga tgatttgaac gtttttaaag attagaatat aaaagattgc
    19981 actagacata tatgaaaaag ataatataat agatgatgta atagatgaca gttcaagaaa
    20041 ttataaaaaa aatttatttg aaatattatc tattacatca ggaataatat atgtaagagt
    20101 aagcttttaa aattctaaag atttagaaag attgaaggga gaaagaaatg agtgaagtta
    20161 agaataccct acaaaaacat ccagttttaa gatattcaac aatgctattt gggatgttga
    20221 tgacatctat aggaataaat gggttcttaa gaccagcaca tctacttagt ggaggtgcta
    20281 caggtatagc aacatctata aactatttga caaatataaa tgtaggttta ttaacatttt
    20341 taatcaatat tccaatattt atcttagggt tcatatacct tgaaaaagag ttttgtattt
    20401 caagtttggt aaatatgatt gtattttcat tattattagg agcaactcag gaaataagta
    20461 atattatacc tatacatgat atattgcttc aaagtgtata tggtggtata ttatctggtt
    20521 taggagtcgg agttgtattt agaactaggt cttctcaagg tggtacagat ataatagctg
    20581 caatactaaa aattaagaag aatatagaaa tgaaagacac tgctttagca ataaatggct
    20641 tgatagtact tacaggaagt tttttatttg ggttggattt agcattatac actcttatag
    20701 gtctatttct aaatgcgtat tcaatgagtt ttattaaaga tgcaatgaat taccaaaaat
    20761 ctgttatggt aatgtcaaat gaagtcgatt taatagcaga agatataatg aagagtttag
    20821 ttagaggtgt aactttttta gatgcagaag gtgcatacac tcatcaaaaa aagaagatta
    20881 tatatacaat agtttcatca aatgagatac caaagataaa agatatagct ttgaagtatg
    20941 ataaaaaggc ctttatatct gtaaatgatg ttacagaagt aaaaggaaga gggtttaaag
    21001 caaaagattt atagaaaatt ttacaagctg ggagtttata atgtaaattt gtatattata
    21061 tctctcagct tatttaaatt tagtatacga aaataaaggt tttattaatg atttgtgtat
    21121 cactttaaaa gatatcattt taaaattaaa attgaaaaaa ttacttaata tgtagaaaac
    21181 atgaccacta ttaggatata ataaatttaa tagatttagt aaaatatcta ggaataacgt
    21241 tggaggaatg gcattatgaa tttatctaaa gaatatgtta atttgtatga taattatttg
    21301 aaatatataa atgaagtaaa caaagagatt agaagtataa agaacatgaa gaaacgtgtt
    21361 ttaaaaaggg gcgattttgc tccagaaaat gaagtattag aattggaagg aaaaacaata
    21421 cgcgatatag atgatgtcga tacaaaaaaa ccaaaaaata aaatctataa attttcaaat
    21481 ggggatgtat atgtagggaa attcttagag ggaaagatgg aggggaaagg ctcatatact
    21541 ttttttgtag atgaaggaac agttatggag tacataggag aatttaaaga agataaaaaa
    21601 aatggaaaag gaaactttat attttcaaat ggaaatgaat atataggtta ttttgaggaa
    21661 gatatgatga gcggtattgg aaatatgtta tatagtagta aagatgaata tattggaact
    21721 tggaaaaacg gaaaaaaaga tggaaaaggt atttataagt ggagtgatgg atgtatatat
    21781 gcaggtgaat ttaaaggtgg aaagatggaa gggtacggta tttgctataa tagtaaaggg
    21841 gaagtacttt atgaaggaga atggaagaat aatttgattc atggtaaggg aacttatata
    21901 tgggaaaaag gtaaaaagta tataggtgaa tttatgcatg gtaaaaaaca tgggcaagga
    21961 actttttatc taaataatga attggtttat gaagggactt ggaaatttga taagccatct
    22021 atatttgata gaacattaga tgaaatattt tccttgagac tgtagcttta tctaattagt
    22081 agataaagct tttttctaat acttactgta aataggtatg aatgatattg attagtatac
    22141 actatcaata ttaacttaat aaatgcaata ttaaaaaatt ttgattgatt ttaagaagga
    22201 gagatatatt gaagagatta attgctttag tgttagtagt acttattgtt ttattttata
    22261 gtcctgaatt attaaaagaa ggatatgatt taaaagaaaa gttttttcaa agtgaaatat
    22321 ggaatgaatt tttagacaaa ataaatataa atgataattc taagaaagat aaaaaagaaa
    22381 aaagtcaaaa tttacaatca aataatactg aaggggagaa cttaggagag tcagatataa
    22441 agaattttga aaaaataagc ttaggagata atttaagcta tgttttatca agtatcggca
    22501 aacctggtag aattgatatt agtgaatatg gttttgattg gtatgtatat aatcaatatg
    22561 gaaaagaatt tgcaatggtt ggacttgaaa atgatgaagt agtagcactt tatagtaatt
    22621 ctattaattc atgtgaaaat caagatataa aactaaatca agatagacaa acagtgagaa
    22681 ctaagattac accattaaaa tataagagaa aaggaaatac cagatatata ataaactcag
    22741 aaaatcaata tgatataata agtaaggaag gtaagtatat tacaattttc tacgatattc
    22801 atgaagaaaa tagagtttgt tcatatttaa taattgataa aagcactgaa gatgagttta
    22861 aaaatttata tccagatgat agcgaagaac ttaaaaaatg ttttgaactt gaagttatag
    22921 accttgtaaa tagtgttaga aatcaaagag ggttaaactc attgagatac agtgaacagg
    22981 caactttgag ttcaagaaaa catagtgaag atatgagaga taataatttt tttgaccatg
    23041 taaataagaa gaatgaaact ccatttgaca gaatgaaaag agaaggtata gtatatactt
    23101 cagctggaga aaacatagca gcaggacaga ttaatgcaat atatgcgcat gaagcttgga
    23161 tgaactctga aggacataga aaaaatattt tggggaacta taataatatt ggagttggag
    23221 taatatttgg aggaagctat aaaacatatt acactcaaaa tttttataaa taactaaaat
    23281 atttgaaagg aagttaaaaa atgacatcat ggaaaaaaaa gacagtatat aagtgcttaa
    23341 tagcagtagt cttgttttgt ggaatagtgt taatatctaa ttttagtaaa gtttctgctt
    23401 tgatgatgga tactaatgga aatgttttga ttaaacatgg ttcaagggaa aaaaagctaa
    23461 tagcaattac ctttgatgat ggtcctcatc ctaaagaaac tagtcaagtt ttagatgtat
    23521 taaagaaata caatgtaaaa gctacttttt ttatagctgg aaaacatgca aaatggtata
    23581 aagaaccatt agtaagagca agtaaagaag gtcacgagat aggaaatcat actttcaatc
    23641 atcctgatat aagtaattta agttctagtc agatagaaga ggagattgtt aagtgtgaag
    23701 acattttgaa agaagtaact ggaaaaaaac cgacattatt tagaccacca tttggaagtt
    23761 atagagaaaa agaattaata gaaattgcaa aaaagcatga ctataaagtt gttttatgga
    23821 caggtgtaga tgtgaaagat tggaaaaatc caggagctaa tagcattgca gataaaataa
    23881 ttaataaagt acaaaatgga gatataatac ttttgcatga ttatgcaact aatgatacag
    23941 ttgaagcttt agatatgttt attccaaaaa tgattgaaaa aggttttaaa tttgtaacag
    24001 tttctgagtt aataaaataa tttttagggg ggagatttac agtatgattc ttatgataga
    24061 caattatgat tcttttgtat acaacctagt ccaatatata gaagaattag gggaaacagt
    24121 tgtagtaaaa agaaataatg aaatcaaaat aagtgacatt gaagaactaa atccagaggt
    24181 aattgtgtta tctccaggac cgtgttctcc aaaagaagct ggtatttgca tagatattgt
    24241 tgagcatttt aaggggaaaa aacctatact aggaatttgt ttaggtcacc aaactattgg
    24301 acatgttttt ggaggagata ttataaaagc acaacagcct gtgcatggaa aagtatatag
    24361 tatcaaccat actaataaag gagtttttag gggacttaaa aatcctttaa atgtcactag
    24421 gtatcattca ctaatagttg actccaatac agttccaaaa gaattagaaa ttacagcaat
    24481 tactgataaa ggggaaataa tggggattag gcataagaaa tatttaatag aaggagtaca
    24541 gtttcatcca gaagctattt tgtcagagta tggtcatgaa atgcttaaaa attttataac
    24601 agaagctaga gaaagggtac atatgtaata tgataagaga gataaatact aaattaaatt
    24661 cttttgagat atttactatt tttagaaatg aacatgacag ttttatatta gacagtgcta
    24721 tggacaaaga aaaattggga agatattctt ttataagtag ccaaccattt aaggtattaa
    24781 agtataaaga tactgatgaa aatcctttag aagttttaaa agaagaacta cataagtata
    24841 gggttgtaaa tgatactaat cttccatttg taggaggtgc tgtgggatat ttatcttatg
    24901 atttaggtaa ttacatagaa aatttaccta gaactgctgt agatgatatt gagatgcctg
    24961 atatgtattt tggattttat aatcatgtaa tagttataga ccatcttgtg caaaaaactt
    25021 atatagctac tccaaacata gatatcgaat tagaagaaaa gataatagat gatatagaac
    25081 aaagaatatt aaaagaagag aaaaaaggta tagatagcat atgttatgaa gaaaaagaag
    25141 taacacctat aagacttaaa tctaatttta caaaggaaga atttaaaaat gcagttcaaa
    25201 gtgttagaga gtacataaga caaggagata tatatcaagc taatttaaca caaaggttca
    25261 gtggagaaac agaacttaca agctttgaat tgtataggga cttaagaaga tttagcccag
    25321 caccatttgg ggcattctta aactttgaag atgcacatat attatcaaat tctccagaaa
    25381 gatttataag atgtgttaat aagagaatag aaactagacc aataaaaggt actcgtccaa
    25441 gagggaaaga taaagaagaa gatttaagac ttcaacaaga acttagaaat agtgaaaaag
    25501 atagagctga actacttatg atagtagatt tagaaagaaa tgatattggg agaatatcta
    25561 aaactggaag tgttaaagtt ccagagctat ttgtaattga accatatgca aatgtaaatc
    25621 acttagtttc cacagttgta ggtgagttaa aagatgataa agatgctact gatgtaataa
    25681 aagcaacttt tccagggggg tctataacag gagcacctaa gattagagca atggaaatta
    25741 tagacgaatt agagccaacg caaagaaatg tatatactgg ttcaataggt tatataggtt
    25801 ttaatggtga tatggatttt aatatagcaa ttagaacaat aatcaaaaat gacaaaaaag
    25861 tctattttca agttggagga ggaatgactt gggattctga cccagatgaa gaatatcaag
    25921 aaacacttga taaagctaaa tccataatga aggctttgag gggatattat gaagaataat
    25981 gtaggttttg atagtagttt aagtaaattt ggaattggtt tgtttgaaac tattagagtt
    26041 aaaaatggaa tagcagtaga tttagatatc catatagaaa gaatgctaag ttcaataaat
    26101 tgtttggatt tagatataaa atataaaaaa gatttcttga taaatgaaat tgttacatat
    26161 ataaaaaaag aaaatgtcat taataaagca cttagaatta ctgtttttga tgaaggttat
    26221 aatatatcta ttagggatat tccatataat caagagacgt atgagaaggg gtttaaatta
    26281 gctatatctc caatagttag ggggaatagt ttaattcata gacataagac tactaattat
    26341 tttgaaaaca tatataccaa aaatatcgca aataagactg gttataatga tggtatattt
    26401 ttaaattcag aaggtgtaat attagaatgt tctatgagca atattttctt tgtaaaagga
    26461 gaaagagtgt acactcctag tgatagatta ccgatattaa atggaataat aaaaaaaagg
    26521 attatagaga tttgtgacga attacatata gagttgatag aaaatgaaat taatatatca
    26581 gaaattagta gttttgattt tgtttttgtc acaaatagtc ttatgggcgc tataaaggtt
    26641 acagaaattg acaaaataaa atttaataaa gaaaatgttg tttttgataa aatagtgaag
    26701 ttattagaat aaattaaaat attaacaatt tgttggagac aaaagatgat aggttgtatt
    26761 tatgaagtat acaatgaaga gataactaaa ataatagaaa accaaaacac tttggctgta
    26821 tatttagtgg gttctagtaa agacgtagat tttactcttt atgatgtaga gataaacgat
    26881 attgatgtgt tcgtttttgt aaaagaaggg gaaaaacaag agagaatact gtttaaaaaa
    26941 aagggaatag aatttgatgt aaattacttt tcaaaagatg gtgtaaaaaa attattatca
    27001 aatagggaat atttctttgt gaaagaaatg aaagatgcta aagtactgtt tgatagggag
    27061 aatatatcac atattataaa agatataagt agaaatattt acttagaagg acccagtaaa
    27121 atgtcattag aggaaaagtc ttttataaag caagatattg gagcaaaaat atcgaattta
    27181 aaaaataaag aaaaatttga tgtttttgaa tatcattttt tgacgaattt atatttaaaa
    27241 gatataataa ttgggtactt taatataaat gataaatggg taccaaaaga caaaaaattg
    27301 ctaaaagttt taaaaaaaga aaataatgag ctttttgaac tagtgtcaaa agtaagtgaa
    27361 aattatgact atcaaagact tttagatgtt tataattata tttttaaaga aattgagaca
    27421 aaagaggtta taaaactaat cttctagtca caaatcaatg gggggagttt aaatgaataa
    27481 agttgataaa gaaaagatac aacatgcagt aagagaaata ctagaggcaa ttggggaaga
    27541 cccagataga gaaggtctaa tcgaaactcc aaatagagtt gctaggatgt atgaagagat
    27601 attctctggt cttagtgaag aaccaagaga ccatttaaaa gttctttttg cagatgaaaa
    27661 acatgaagag ttagtattgg taaaagatat tccattttat tcatgttgtg agcatcatct
    27721 agttcctttc tttggaaaag ctcatattgc ttatttacca aaaggaggaa gacttacagg
    27781 gctttctaaa cttgctagag taatagatac attggcaaaa agaccacaat tacaagaaag
    27841 aatcactaaa aatgctgcag atataataat ggaagaatta caaccttatg gtgtcttagt
    27901 agtagtagaa gctgaacata tgtgcatgac aatgagaggc gtaaaaaaac caggttcaaa
    27961 aactgttaca tcagcagtga gaggaatatt tgaaaaggac attgcgtcta gagccgaggc
    28021 tatgagtttg attactatga agtagcaatt tattatagat ataatcattt attgagataa
    28081 aatttaattt aagggagtgt cattaattgt gtttgattat ggtaaaagaa cttatataat
    28141 gggaatatta aatgtaactc cagatagttt ttctgatgga ggagatttta ataacttgga
    28201 catagcaata caacatgcaa aagatatggt tgaccaaggt gcagatataa tagacctagg
    28261 aggagagtct acacgcccag gtcatagcta tgtagactca gacgaagagc taagaagagt
    28321 aatccctgta ataaaaaaac ttaaacagga attggatata ccaatatcaa tagacactta
    28381 taaggcagat gttgcagaag aagcattaaa attaggtgtc actatggtaa atgatgtttg
    28441 gggtcttaga aaagataaaa atatggcttc tgtaatagga aaatatgatg cagaagtgtg
    28501 tataatgcat aatcaagatg ggacaaacta tgacaaagat ataatggagt caataaaaga
    28561 cttttttaag gtaagtatag aaatggcaat gagttgtggt gtaaaaaaag aaaaaatagt
    28621 acttgaccca ggtgtaggat ttggcaagga ttttgaacaa aatattgaag tattgagaag
    28681 attaaatgaa ttaaaagatt tagggtatcc tattttgctt gggacatcca gaaaatcagt
    28741 aataggaaaa gtacttccag ttgagcctaa aaaaagatta gaaggtacaa tagctactac
    28801 tgttcttggt ataagagatg gcgtagatat agttagagta catgatgttt atgaaaattt
    28861 aatggcagct agaatgactg atgcaatata tagaaagtag gtataatata atggataaaa
    28921 tattacttag taatctagga ttctatggat atcatggtgt tcttaaagaa gaaaattttc
    28981 taggtcagaa attctttgtc gacatggaac tttatattga ttcaagagaa gcaggtctta
    29041 gtgacgatat aaataaatct gtaagctatg ccgaggttta caatgttgtt aaagatatta
    29101 ctgaaaataa acaatttaat cttttggagg ctttagcgga aaatattgct gaagaagtct
    29161 tgaataaatt tatacttata aatggagtta tggtcagagt tagaaagcct gaggctcctg
    29221 taaatggtat ttatgactat tttggtgttg agataaggag aacaagagat gaataaggct
    29281 tatttgggaa taggtactaa catgggtgat agatttgata atttatcaag agcatgtgaa
    29341 cttttgaaaa atagtgattc tatatataaa gtaaaagaat caagcttata cgaaacaaaa
    29401 ccatggggat atacagaaca agcagatttt ctaaatatgt gtgtagaaat agaaacagag
    29461 tttgaacctt atgagttgtt agaatattgt caagaaatag agagagaact acacagagag
    29521 agaatagttc attggggacc aagaactata gatgtagacg tactattttt taatgatgta
    29581 gtttccactg atgaaagact aacaatacct catccaagga ttcaagatag agctttcgtt
    29641 ttgataccac ttatggattt aaatgaagaa cttataataa atgaaaaaac aataaaagaa
    29701 catttaaacc tcctatctgc tgaagaaaga gaagaagtaa aggagcttgt aggttatgaa
    29761 agaaaaccta tttaataata attcaaagat agagataaat acaaataaca aaaagataac
    29821 tgtaaaaaaa gataaactta taatagctgg accatgcgca atagagagtt atgagcaact
    29881 tttggagact gctaaatttg taaaaagtca aggagcaaac attttaagag gtggagcata
    29941 caagccaaga acatcgccaa attcatttca aggattaaaa aaagaaggtc ttgaaatatt
    30001 aaaagcagtt aaagacgaag ttggtatggc tgttattaca gaacttatgg atgtaagaga
    30061 tatggacgaa ctgtatagta taagtgatat aattcaaatt gggtcaagaa atatgcaaaa
    30121 ctttactctt ttaagtgaag tgggaaaaca aaataaacct gttatgttaa aaagaggaat
    30181 agcatccaca attactgaat ggataggtgc ttctgaatat atagcaatag aaggaaatag
    30241 taatattata atgtgtgaga gaggtataag aacgtataat gattatacta gaaatacatt
    30301 agatttagct gcggtaccca ttattcaaaa ggaaacaggt cttcctgttg tggtagaccc
    30361 aagccatgcc actggagtta gatatttggt aaaacctatg tcactggcat cttttgcatg
    30421 tggagcagat ggaataatgg tagaagtaca tcctgaccca gaaaatgctt tgtcagatgg
    30481 tgctcaatct ctttgcttta atgaatttga agatttaatg aaatcaatta ataattatta
    30541 aaagaggcaa gagcctcttt tttattcgta atcgttgcta aggtaaattt ataagttata
    30601 atttgatagt tagattattt aatataaata aagatttaat attgatagaa ttaatataaa
    30661 aaataaaaat tttttgatta ataagaagga tatcaataaa aaaagtcgaa ttgtgtatat
    30721 tgagtgattg acaaatttag aatatagtgt agaatagaaa tgttaacaca aaaaattaca
    30781 ccagaaggtg ataatatgaa tgatataaaa gacattattg aagaaataaa agctagatgc
    30841 gatatagcaa gtataatatc tgaatatatg aatataaaac aatctggagc taattacaaa
    30901 ggattatgtc catttcatgg cgaaaagaca ccttcttttt atataaatac atcaaaacag
    30961 atttataagt gttttggttg tggtgaaggt ggagacgtaa taaattttgt catgaaaatg
    31021 gaaaatttgg attttatgga tgctgtaaaa attttagcaa ataaatgcgg tatagaaatt
    31081 aacactaaca tgaatgaaga aactaggatt aaaatagaaa aatctaagaa atttcaagat
    31141 attcatacgg aagcagctag attttatctt tcaaacttat tgggaagcaa aaatcttggg
    31201 tatgaatatt taagaataag aggactagat gataaaatta ttaagaaatt tggtttagga
    31261 ttttctttgg attcatggaa ttcacttatg aatactctaa tcagtaaagg atataaaaaa
    31321 caagacttat tagagtgcgg tctaattgca aaaaatagag atggaactaa ttgctatgat
    31381 aaatttagaa atagagttat gtttcctata ttcgattata gaggaaatat aataggattt
    31441 gggggaagag tattagacga ttctttgcca aaatacttaa attctccaga cacattaatt
    31501 tttaataaaa aacagaactt atacggatta aattttgcta gaaaaaattt agaaagtaaa
    31561 actatagtct tagttgaagg atatatggat ttaatttcac tttatcagta tggtataaaa
    31621 aatgtagtag caacccttgg aacagccttg actgaacaac aaggcatttt aataaagaga
    31681 tatgcggaca ctgctattat atcttatgat tctgatgaag caggaataaa agcgacttta
    31741 agagctatag atatacttac taaattaggt atcaatgtaa aagtgttaga cttaaaagat
    31801 gctaaagacc cagatgaatt tgtaagaaaa tacggactta gtgattataa aaaagctatg
    31861 gatgtttcta ctcactacat aaaatataaa atagatcatc ttaaaaaaga atttaacatt
    31921 caaaaagatg aagaacgagt gaagttcgca aaagaagctt ctaaaattat aaaacaattg
    31981 acaagtcctg ttgaaattga tttttataca aagtatttaa gcaatcaaat agatattaat
    32041 gtcgagtcta taaagagaga agtttatggt aaaaattaca ataagccata taataacaaa
    32101 aatcaaaaga aaatagaaga aaaagtaatt gaaaaagtcg aagtcagaca agacggaaaa
    32161 caattagttg aagaaactct aataaaaata atgttagaag acaaaaaaat tagagaaata
    32221 gcgttactta aagtagaaga aagtgatttt ttattgaaag aaagtaaaga aattttaaat
    32281 tacatgatta aaaatcaaga attggacaaa ataactattg acaaattgaa aagtttaaac
    32341 atatccgaag aatacttaaa agaattaaat tcaatttctt tgaatagtat aaacttagag
    32401 aacacaaaag aaatagaagg aataataaca aatatcagaa aaaactcatt agaagagcaa
    32461 attaatagct tgttgagaga acaacaagaa ttagaaaata ataatgatat gaaagaggta
    32521 gatggtagag ttatggaaat tgctttaaaa atagttgaga taaataaaat tttaaagagc
    32581 ttgtagagaa aggaggtttg agtgtggaaa ataaatcgaa taaaaaagag ttaaaaaagg
    32641 ttactgctaa gacattaata gaaaaaggaa aaaagcaagg ttctctgaca cttgcagaga
    32701 taatggaagc tttttcggag actgaacttg ataaggatca agtagaaaat ctttatgaga
    32761 ctttaggaaa tttgggaata gaaataacag aaacaaaaaa ctataaagct gatatagatt
    32821 tttcggttgc tgatgacgat ttaagtatag gccacttaga tgaagatgca gaggcaattt
    32881 cacatgatga ctcttctgca atagaaatag aaactgtgga tttatcttta ccaaaaggga
    32941 taagtataga tgaccctgta agaatgtact taaaagaaat aggaaaaatt cctctactta
    33001 aaccacatga agaagtagaa tttgctagaa ggatgcacga aggtgatgag atagcaaaac
    33061 aaaggttagt tgaagcaaac ttaagactag ttgtaagtat agcaaaaaga tatgtaggaa
    33121 gaggtatgct tttcttggat ttaatacaag agggaaattt aggtcttata aaagcagtcg
    33181 aaaaatttga ctatacaaaa ggatataagt ttagtactta tgcaacatgg tggataagac
    33241 aagctataac acgtgctatt gcagaccaag ctagaactat aagaatacca gttcatatgg
    33301 tagagactat aaataagcta ataagagtat caagacaact acttcaagaa cttggaagag
    33361 atccaaaacc agaagaaatt gcaaaagaaa tggaaatgac agaagataaa gtaagagaaa
    33421 taatgaaaat agctcaagac cctgtgtctt tagaaacacc aataggagaa gaagaagata
    33481 gccatttagg tgactttatt ccagatgatg atgctccagc cccagcagag gcagcagcat
    33541 attctttatt aaaagaacaa atagaagatg tacttggttc attaaatgat agagaacaaa
    33601 aagtattaaa gcttagattt ggtcttgagg atggtagagc cagaactctt gaggaagttg
    33661 gaaaagagtt tgatgtaact agagagagaa taagacagat agaagcaaaa gcactaagaa
    33721 aactaagaca tccaagtaga tcaaaaaaac ttagagatta tttagactaa aaagatacat
    33781 cattgatgtt tttgtgataa atgccatgag ataagtgata tctcatggct attttgtagt
    33841 aaagatttaa ttttattttt gtagttattt cagtctaata tctataactt ggattaaatc
    33901 ctataaataa agggaattga aaggagaatt atatttgaaa ttaacagata gattattgaa
    33961 aatagcatca ttagtgtcag atggtaaaaa aatagctgat ataggaacgg accacggata
    34021 cataccagta tatttgttaa aagagggaaa agtaccattt gcagtgctag cggatgttaa
    34081 taaaggacct cttgacaatg cacataaaga agtcatacag aacaatcttt tagataaagt
    34141 tgatttacga ttgggttctg gtatagagat acttgaaata ggggaagtag aagaagtgat
    34201 aattgctggt atgggtggaa tcttgataag tgagcttctc gaagctaaaa aagaagtagc
    34261 acataatgta gaaaaattaa tattacagcc aatgcaagca caagaagaac ttagatatta
    34321 ccttttaaac aacggatatg aaattttaga agaagtttta gtgagagaag attttagaat
    34381 atatgagata attgttgcca aatatacagg taaaaatact ataatagaag atgagatata
    34441 ttatgaggtt ggaataaagc ttttagaaaa caaagattct ctttttaatg attttataga
    34501 aaaaaagata aagacatata gttctatagt taataaatta gaagggaaaa atggggaagc
    34561 tatagataag aagagaaagg aaagcgaagt tgcaataaaa aaacttgaaa atttaataaa
    34621 ataaatagga ggtatatatg ctattaaaga gtctaactag aaaaattgaa aaaaagtatc
    34681 ctctaaactt agcagaggat tgggataatg taggtcttat agttggagat tttgatatgg
    34741 atgtaaaaaa ggtattggta tctttagaag ctaatgaaga tgttattgat gaagctatat
    34801 ccaaaaatat agatttaatt gtgacacatc atccttttat atttgggaaa attaataaaa
    34861 ttaatagtgg agatttaaaa ggcagactta tccaaaaact tattaaaaat gatatatcac
    34921 tttattctat gcatacgaat tttgatatag catttgatgg tctaaatgac tattttatgg
    34981 aaattatgga atttggtaat tcaaaggttt tagatataac taaaagcgaa aatttgtata
    35041 aattagcagt atacgttcca cataactatt cagatgaact tagaaaggtt ttaagtaatt
    35101 caggagcagg tcatataggg aattatagtg attgtacgtt ttcaacagaa ggtgaaggac
    35161 aatttaagcc attagaaggc tctaatccgt ttcttggaag tgtaaatgat atagaaactg
    35221 taaatgaagt taaaattgaa actgtagtac cacagaagct tttaggtggg gtaataagtt
    35281 ctatgttaga tgcacatcct tatgaagaag ttgcatatga tttgtacaag ttagaaaata
    35341 aaggtgagat atttggacta ggtagaataa gtaaacttga taaaagtatg acattagaaa
    35401 gtctttcaag gaaaataaaa gagaaattaa atatgaaaca cataagagta gttggaaacc
    35461 ttagtacaga cataacgaaa gtagccgttg taacaggagc tggttcagaa tttgtaaaaa
    35521 aagctaagag acaaggtgca gaagtactaa ttacgggtga tgtaaagtat catgaggcac
    35581 aagatgcact tgatattgga atgtgtatag tggattgtgg tcattttgat acagaagata
    35641 tattcaaaaa tgttatgaag agatttttag atgaatttag tgaaattgaa gtaataaaga
    35701 gtaatgtata tttgaatcca tttagtacta tttagcagtg tttgataata aaaatactat
    35761 ataaaaaata aatttgagtt taattagggg gataatgatg aaagtagcag taatttttca
    35821 tagtgtttgt gggagtacat atttattagc tagagaatat aaagaagcgc ttgaagaaat
    35881 gaatatcgag gtagatatat ttagagtaag tgacgaagtt gcaaaaacac ttcctcaata
    35941 ttatttgata aattccaaag aatacaaaga cgaatttgaa agtataaatg taattaaatc
    36001 tggaaaagaa atattagatt atgatgctat atttatgggt tcaccaacat attatggaaa
    36061 tgtatctgga ccaatgaaga tgtttatgga tagtttttct gatatttggg taggtgctcc
    36121 tttgagtggt aaaatatttg gttgttttgc tacagcaggt tcacaacatg gaggaggaga
    36181 attagctttg caagctatga atatatttgc ccaacatatg ggaatgactc ttttgtcagt
    36241 tccatgttca gtaagaggag gttatccagc gtatggaata cttcatatag caggagataa
    36301 ttctgatata aggccaaatg atgatgccaa gataggtata agagattacc taaaaagact
    36361 taacatataa gaatatcata taaaaatacc taaaagcgac tgtatatact tttaggtatt
    36421 tttacttaat tataaacgtg aagctttacc agaaatatgg tcaatttcta ttttaacaac
    36481 agttgttaag ttcatatcct tctctatgta cttcatgcct tcttcaaaga atcctttaga
    36541 atattttttt attatttcta caagagcatt tttcttttct tcattttcta cagtgcaagc
    36601 ttttccaaaa actatagcac tagaataagt agtactaaat ttatctggaa taacactttc
    36661 attagcaact actaaaaaag aaactttgtt gtttttcgat atattatcta acttatgccc
    36721 atttcttgca caatgaaagt aaatagaatc attaaagtat acataattta cagcaactcc
    36781 ataaggataa ccattttcag atatagtgga aaaagtacca aattcaccat tttttaatac
    36841 ttcaacagta tcttcttttg tcatttctct ttttttcaat ctcatttctt taaacataat
    36901 atcatccttt ctaattttga tgcttaaatt aaattttaga acatttataa agattatgct
    36961 atgtttttta caaattttaa gaaaaaaata tagtttaaga taaaaggtag tgaaagagaa
    37021 gctatttgga ggtttattta tgatattatt gttagagtag tacaaataat actaataaat
    37081 ttaatagctg gtttcttgtt atttaaggta gtaagcatgt ctataagaag gggaagttat
    37141 cgagggtaga agttgttgtt tataacgata ttataaagaa aatattatta tataaaataa
    37201 taaatagtaa agaggtatca agttgaatat aatacttaat acctcttcaa aattaagtta
    37261 taaaagtgtt gtgggaattg tcctatacat ggtaaggaga taataaagaa tgtatcttta
    37321 aagtctcaac taaacagcaa aatttatcca acaaacatac aataaatgct tctttgtatc
    37381 taggtggctt aacagtaagt ggccacatgt gttttaaaat tatgtccgtt tctttttcat
    37441 ttatttgaaa aaataaagtt gcatttttta atgcttctct agggtgagta aatccatgta
    37501 atccttttct atcacctttg taatgccaat cataaagaaa aaagtcgtgt agtaaagctc
    37561 ctctaataac actttgaata tcaacactta agtgcaattt tttacataat agataactat
    37621 aatatgctac tgacaaacta tgttcaagac atgaaacatt gccatgatga ggaaatgttc
    37681 tcataagttg aactttttca gaagaaagta tctcagaagc acattcatta aaatatttga
    37741 tttcagcttt tgacaacatt atgaaaatct ccattcttag taatatataa atactatact
    37801 tcaacgataa tatgaaatat ttcttatcct acaaaatata atacccaata atacaagtaa
    37861 gatacctttt atggtatttt aaagtaagag agattttatt aggctaaaca atgaaaaaat
    37921 taaaagtaaa gctatagcaa tatatagtat ttttccaaga ggattgccaa aatttatagt
    37981 ccatccaata ccaaaccttt tattaaccat taatgatggg tcatttggat tattatacat
    38041 gatgcctgct atccaatact tctcatcttc ttctggagaa taagatgagt tgaattttaa
    38101 atttggggaa ttaatatatg ttgctattaa ataaattata actaaaagcg ttgtaatatt
    38161 catagcagtt aatagttttg tttttattga taaaagagta gtaaaaaact gtgttgtcat
    38221 tataatcatc aatattaaaa acaaataacc aattttattt aaatatttaa tgttttcaac
    38281 tctactttct tcaattctat ttgtatctat cttaatcctt gactttatgg atccaatgga
    38341 agttatatat aataaaacca ttaaacaaaa gtcagtaccg attaatttaa atacattaag
    38401 aaatgattta tctataaaaa catcaggaga cccatttatg ttccaatgag tggcaattga
    38461 gtctggcaat tgattataat taaatgctat aaaaatactc attcccaaag taaatagtat
    38521 cggtattaaa tagattattt taaatttttt tattatttta tttttcttat ttataaaatc
    38581 catatctact attaattttg aatctatatc tatatgacct aatttagcat aaatttctga
    38641 tttaagcttc ttagcttttt tatgtgtatg aatatatagt atactttcat ataataaaaa
    38701 acctataata ccaataaaat aggaaagttc caatttatta aatgtaaaga ccagaataaa
    38761 aactaatata aagtctataa taaaacctaa agataatagt tttttaaatt ctttatctaa
    38821 ctttataaag taattataat ttttatgctc ttggtctata gaaactccat agatgtgttt
    38881 tttacctaca aaatagggta taaaataaaa tgtagcatac atcaaaatta gaactggaaa
    38941 tattgtaaat agacattcaa tcttactcat aatatttcac ctcatacttg tcaaataaac
    39001 ttgaacttag tgacaaaaat tcttctttac taatacctga taaataagat tttgccacca
    39061 ataattccaa gtcaagttta tttttttgac gagttgtatt atctattttc attggtagtt
    39121 gagctacaat agcacctttt cttctatcaa tatttaagaa tccctcttct tttaattcat
    39181 tgtaggattt gttcactgta tgtaaattta caccaatatt ttcagccata cttcttacag
    39241 atggaagtga ttcatttatt ttcaattctc ctgaagctat agccttagtt atttcttctt
    39301 taatttgtac ataaatagat ttatcactat taaaatctaa atttaaaacc ataaaaaacc
    39361 tccattatgc tttataaatg aaaacgattg ttatatacat agtataacat aaaaaataat
    39421 aacaaacaca aaatataagt ttatattttg taaaaattgt aatttaaaaa ataacataat
    39481 ttttattaaa ataatacaga tactatttat agatagaaat aaaaattaga aaggagaaaa
    39541 aatatgttaa catatttaat gaaaagacaa atgcataaaa aagatgatgg aatgagtaaa
    39601 ccaatgatga ttacaggagc tgtaattaca ggtgtaggag catatgctgt atataaaatg
    39661 gtaaaaagcc ataaatctaa ttctcaaatg gtcaatacaa attattatgg aaatagcgat
    39721 tatgatgatt ataaatatga cgaatttgat tatgactgtg attgtagaga cacaaaacaa
    39781 gattatgaat atgaagaatt aaaaaaaaaa gtaaaagaat ttaattcaag aagaataaat
    39841 tgtgaaaatt gtccacatgt ttctcaagaa gaaatgatgc aatatgtaaa tagtgttaaa
    39901 gatgtaaagg aagagcttga tttacatgaa gatgctaaaa aagataatat ttacaaagaa
    39961 gatgaataca aaaaatatga agaagaataa gtaatatttt gtataactga acaaatagta
    40021 atgtcaaatt atttaatatc tagttttaat atgtaagttg taacttaact tgaaagaatc
    40081 agtatcattc tttagatagt taagtaacaa cttacatttt atttttataa attttaatag
    40141 ttctttgtga ctattttgta attttagaaa tattttagta tccagggtaa ttgtgtataa
    40201 taaaacttgt atagatagat agtatgaaat taagcagata gtttaaaatt aaaatgaaaa
    40261 gaaaggaaat agttatgtta aagttgttga tagttgaaga tgatagtact atatcttttg
    40321 gaataaagta tgctttagaa caagaaggat ttagtataga tatatcaaag gatttatcta
    40381 gtggaaaaga aatgatttct tctaatgaat atagcatgat actgttggat gttacattgc
    40441 cagatggaac tggttatgag ctttgtcaat atataagagg attctctcag gttcctataa
    40501 tattcttgac tgcttgtgat gaagaagtaa atattgtaat gggtctcgat ataggtggag
    40561 atgattatat aacaaaacct tttagaatta gagaattaat atccagaata aaagctgttt
    40621 taagaagaaa aggtaatact tcagaagaaa ataagaaaat acttaaattt ggagatttaa
    40681 gtatatatac attagaagct agagtatata aaaatgataa agaaatattt ctgacatcag
    40741 ttgaatataa acttctttta attcttatac agaataaaaa tactgtttta agtagaactc
    40801 aaatattgga aaaactttgg gatgtaactt atgattttgt aaacgacaat actctaactg
    40861 tttatataaa aaggcttaga gaaaaaatag gtgatgattt atgtgaacca atctacatag
    40921 aaactgtaag ggggattggt tataaatgga taggaagtga aaatagtgtt tctatataat
    40981 ccagaagtga agaagttttt aagtaagtat gttactttga tgtttattgt tatcataata
    41041 tctattggat ttagcataat caacgtgtcc ttaactaaag agatggttgt aagaaataat
    41101 caggctataa taggaacttt atcaagcaag tatccaaact tagaaagtca gatagtagac
    41161 ataataactc aagggaagtc tatggagaac atagattatg gtcagaaaat attaattaag
    41221 tataattatg ataaatctat taggataaac tctgagccta ttatttctaa attagtttta
    41281 gagactatca aaataaatat aatattagta tgtataatat ttattttgat atttgtgttg
    41341 attgttagat attttacgtc aatttacaat gatttatcag atatgactaa gtatgtttac
    41401 tatagttcag agggtaagcc atttgatatg aaaaacaaga atcaagaagg gcagataggt
    41461 cttctaaaaa ctgaactctt aaaaatgacg acaattctta atgaaaaggt tgaactttta
    41521 aaaagagaaa agatattttt aaacaataca atttccgata tatcacatca attaaaaact
    41581 cctatgactt ctttaataat gttgaatgat ttattatata atgatgtacc atacgaagta
    41641 aaaatcgatt ttttaaataa gataaaaaat caattaaata gaatggattg gttgattaaa
    41701 agcatgctta aattatcaaa ggtagaagca aaagtgataa attttaaaaa agataaggta
    41761 aaatttagtg aactcataca tagagcaatg ctatctatga aaatacctat ggagattaaa
    41821 aatcaaaaac tgactataga gggaaatgat aatgtatctt atattggtga tattgattgg
    41881 tccgtggaag cattagtgaa tataatcaaa aattgtgtag aacatactcc tgaatttgga
    41941 aatataatta taacctataa agaaaatcca ttattttctg aactaataat aaaagatgag
    42001 ggtgaaggta tagataaaaa agatatacca catgtattta aacgattcta tagaggaaga
    42061 agtagttcaa aagaagatag tgtaggaata ggtcttgcaa tgtcaaaatc tataatagaa
    42121 agccaaaatg gcgatatata tgtaaacagc gaaaaaggta agggtacaga atttcatata
    42181 atatttcata aaatgtatga tagtgactaa tctgtaatat ttaattcact ttatagtaat
    42241 agccaaaacc taatatatga agtatagaat ataaatattg gaggtagtta tggaaatttt
    42301 aagagtagaa aatctaacta aaagttatgg taaaaatgaa acaaaagttg atgctataaa
    42361 aaatgtaagt ttatcagttg aaaaaggaac gtttatagca ataacaggtc caagtggaag
    42421 tggtaaaagt accttattac atttattagg aggagttgac agaccaacta gtggtaaggt
    42481 ttatataaat gatgttgata tatataattt aaaaactaag gatttggcga tatttagaag
    42541 aagaaatata ggtcttatat atcaatttta caacttaatt ccagtcttaa atgtgaagga
    42601 aaacatattg cttccagcag agcttgacaa tagagatatt gatagagaat attttgatga
    42661 tttaatgaaa acattaggtc taattgatag agaaaagcac cttccaaacc aagtatcagg
    42721 aggtcaacaa cagagaacct ctataggacg tgcattaatt aatagacctt ctataatatt
    42781 agcagatgag ccaacaggta atttagatag taaaaattca aaggaaatat tagaattgtt
    42841 aaaattgtca gtaaaaaaat acaaccagac actaataatg ataactcatg acaaaaatat
    42901 ggcacttcaa gctgatagaa ttataagcat agaagatgga atcataaaaa gtgatgaggt
    42961 gatgtagatg aatttatata catcactaac tattagatac ttaaaacaaa ataaaagaag
    43021 aaccattgta accataatcg gtataatact ttcaacagct ttaatttgtg gtataggaaa
    43081 tatatttgaa agttttatgg attatcaaat gagagaaact ataaagaatg atggaagttt
    43141 ccatgttaca ttttatgatg taaatagaaa aaatgtggaa tatgtaacga aatcagctga
    43201 aatagaaaaa catgcattta ccaaacaact tggatattct aaattagaaa atagtgaaaa
    43261 tgctatatta agtataaagc aatatgataa aaatgcaata aatggatata aagtttctat
    43321 aaaagaaggt agatttccag ctaaggaagg agaaattgtt ttaagtgaaa gtattattaa
    43381 tcttatagat gataagttaa aaataggaga taagataact ttaaaagtag gggatatgtt
    43441 tgactcaagc aagaaaaaga ttgatagtat gacatttcat gaaggtgatt atatagcaaa
    43501 tgaaaaagat agaactttta aagttgttgg tataattgaa aaacctgggt ttgaacgtta
    43561 taatggaatt gcaacagcta tagcctactt taatcctatg aataattaca atgatacaat
    43621 taatgtatca gtagcagtaa aaaatcctaa agatgtgtat aagataagta ataaaataag
    43681 taaaaatata tattcaaata aagataaaga agcagtttct gatatgataa aatacaatga
    43741 acatttattg aggttacaag gtgctagtaa gtattcaaat ataaatagct ctataaagtc
    43801 tataattata gcagtaacta ttttagtaat cgtatgtaca attgctacag tgtacaattc
    43861 ctttagtatt tctataaatg aacgaaaaaa acagtttggg atattaaatt ctataggtgc
    43921 tacatctagt caaattaaaa gattagtttt tatagaaggt attataatta gtttaattgg
    43981 tataccaata ggactaatat ctggaacagt agctatagac ttattgttta aaattataaa
    44041 taagtatttt actgaatcgg ttgttactaa gatgagtttg cagatagtgt ataatccaat
    44101 aataattatt gttagtataa taatagtttt gcttacaatc tttatatcaa ttctactacc
    44161 tgcaatatcg gcttctaata tttctcccct taatgtgatt aaaaatactg gagagtataa
    44221 agtgggcaaa atcaaaagct caagacttat taaaatgatt tttaaaactg aaggagtact
    44281 tgcatataaa aatattagga gaaatagaaa aaaatttata ataactcttt tttctttaat
    44341 ggtaagtata ataatattta tatcttttag tggctttaca ttacttttac taagaggtga
    44401 agaaattaga aattctcaaa gaaaatatga tttgtattta actgcaaaga acacagctag
    44461 gtctgtagag gatactataa gtgaactaga agatattgat gggataaaaa actttgaatt
    44521 ggcaacagga caatatgtgt ctataagagt aagtgaaaat aaaataaata aatccaagga
    44581 agatttaatc aggaaatatt atcaaaaata taagattgga gatagttatg aatatgattt
    44641 cagtaataat gaattaattt ttccaggaga ttttgcagta aaaaacaaaa taaataactt
    44701 agtacagggc tcttttaata aagagagagc aattgaagaa aatggtgtta tattagttag
    44761 aaaaagtgca attgaagaaa atgggaagaa aggtgtagta gaacttacca actataaagt
    44821 aggagataca gtaaattgtg aatatcttga tgaaaatgcc ttaagtaaaa aagtaaaggt
    44881 caagattctt gctataactg acgaagaacg tctaggtctt ggttatcaaa atatggggtt
    44941 acaatttata acttatgatg aagttgctaa aaatttaaat ttaaaattaa gtaaaagcct
    45001 tatatttatt gattcaggag gaagcattaa gactaagaag gaaatagaag ctttggcaaa
    45061 taaaaataat tttaactttt atgatgaaag tgcaagcaat gaaagtgaaa aacaagattt
    45121 aaaggttatt aaaatatttg tatatggttt tatagtagtg atatctttag tgagtgttac
    45181 aaatattcta aatacagtaa gtacaagtat aaatctaaga aaaagagaac ttgcagtaat
    45241 acaatctata ggggttactc ctaaaggatt taggaaaatg atatatttag aaagttttat
    45301 atatggtata ctgtcattgt tgtttggaat accaattagt attggtataa ttctaattat
    45361 gaataaactt atttcaggtg taattgaatt ttctccgatt ataccgtgga cagccatagt
    45421 tatctgtata gtaagtgttt ttataataac ttttatagca gggtatatac caatgagtaa
    45481 attaaataaa gaaaatataa tagataatat aagaagagaa agtatataga tataaaaata
    45541 attatagaag gaaaggcaca ttatggaaaa attgagagtt attgaaatga taaaaaataa
    45601 tcccaatatt atagctacaa tagataatcc aactgatgaa atgaaattgt tagctattaa
    45661 agaaaatggg cttgtactag aatatataaa taatccaacc agagagatgc aagaactagc
    45721 aatagataat aatattagag caattaaatt tatagataat ccaacagaag atatgatgat
    45781 aaaagcagta aatgagggat ggagtatact ggattatatc aaaaacccaa ctgataaagt
    45841 tattgaaatg gctataaatc aagcaggatg ggcaattaaa tatgctaaaa atccaagtga
    45901 agaattacaa ctgttagcag taagaaaaaa ttatgattcg ataaagttta taaaacaacc
    45961 ttatgaaagt gtacaagagg aggcagtgag aataagttat gatgccttga gatatataga
    46021 ttcacctagt tataatgcag aacttatagc tataaaaaat aatgaagcag ctattagctt
    46081 tataactgat ttagataaaa ataaaatgtt ggaattttta aaagtgaata ttttagtgat
    46141 taagtacatt ctgaaaaaag tatctaaaga tgaattagag agtgcattaa aagaagtatt
    46201 atccaaagaa gatgttgagg aaaagtatat tagagatttt ttgaattgta gtgttataga
    46261 tagaaatagc gaaagtatac caatagataa aattatgttt atttataaat atggaagtaa
    46321 gacagcaaaa aaaatagctg tagatgagaa attaaagatt atgtaggaga gagaaaaatg
    46381 aattttatag acacattaaa aagcgttgac ttaatattat ccactggaga agtaccttta
    46441 atagtaggtg aaagtggaat aggaaaaaca gctttagcaa ataagcttgc caaagaaaat
    46501 gattggagtt taattgttat tgatggaaac ttacttaaag agggtgaaat aggtggtctt
    46561 ccaactatag aatcttatgt aggagttaat tctaatggat ataagactga aaagaaaaca
    46621 acagtgtatg ctgttcataa caaattaaga gaaattgatg aagagatatc taaaagtaag
    46681 acagttcttt tatttataga tgagataaat cgttgtgagc atacagtaca gcaagaactt
    46741 atgaatttaa ttttaaatag agaaattaat ggatataaac tgcatgatga tgtaaagatt
    46801 ttagcagcaa tgaatccatc aagtaaatat ggttcagatt ttgattatca agtggtagat
    46861 atggatgcag cccaagaaaa taggtttgtt tggttaaata tggagagtga ccatacacaa
    46921 tggataaaat gggcaataga tgagggtatt gaaagaaaag ttatcgagtt tatttccact
    46981 tttccagaat atctgcataa aataaatgaa gatgatgtaa gagccactcc tagaagttat
    47041 gagagagtat ctaaaattta taaagtttat aaagaaaaaa ataattctat tcctagagca
    47101 gtatttttaa atgttgtaaa gggtaatgta ggtaaagtta ttgcagaaga atttataagt
    47161 tttattgaat caaactctga accattaata tcctatgaag atgttttttt aggagaatct
    47221 attgatgagt ctattgtaga aagagtaaaa aatgagagtc atacaagact ttatctatca
    47281 gctatgaaca ttctaaaaga cttagaatta aatattaaga atgataaata tgaatcaaac
    47341 cactatatta atagatttat tgaattttta aaaatgtatc ctgtagattt aatgataggt
    47401 attatgaagg atattagaaa cagttatatt gaagtatata aaaaagctat agaaaatgaa
    47461 gagtttgtaa aatcatattt tgaatcttat agtttaataa ggggataatg tatggaaaat
    47521 tattttgata aacaagcaaa agagctttat aataaagcaa gtgaaattat aaatactcat
    47581 tctatgctaa aggcaaatcg tgctaatgag aaatttgata tagatatacc acaagatttt
    47641 aagtatgagt tttttagtct tgtagataaa gtgaatttaa gtcttatgga agaagaagat
    47701 aatttttatg gatatttttt gtttcagatg tcaagagaaa taagatttga tataagtagt
    47761 cctacaggtg tgaattttaa gggagctaaa tatattatat attttaatcc aataatcttt
    47821 ttaacgcttg acataaagca aatggagact acaattaaac atgaaattca tcatatatta
    47881 tctatgcatt tgatgagagc aaaggagtta aaaggaaagt atagtacatt agcaattaat
    47941 atggcaatgg atatagtagt aaataaattt ctaaacaatt taccaccata tgcaactacc
    48001 ttagagtggg taaatttaaa atattcctta aaacttgaac catatgaacc ttttgaatat
    48061 tatgtagaaa agattcagac tgaattagat ttgatggaag tggatgaaga tggtgaggaa
    48121 gatgatagtg ataaatatgg agatatagaa attgattata gccccgaaag aactcatgat
    48181 atatgggatg aatccaatga gatagatgaa aataccctta gagaatttac tgaaaaattt
    48241 attaacagtt ctcaaaaagg taaagttcct gcttatttag aaagtatgat atcatcactt
    48301 aaaaatagta agggtgaatt gccatggaat ttatatctta atagaataat gggaacagtt
    48361 gaaaataata aaaagaagac tataacaaga agaaatagaa gacaaccaaa tcgtttagat
    48421 ttgaggggag aacttagaag tcataagtca gaaatagcag ttgcacttga tgtaagtgga
    48481 agtattagtg atgaagagtt taaacaagct attaaagaag ttcttagtat agtgaaaaac
    48541 tataatcatg aaattacaat tatagaatgt gataatgaaa ttagacgtgt atataaagtg
    48601 agaactgaaa aagatataaa agatagaatt aggataggtg gaggtactag attttctcca
    48661 gtctttgagt atgctaataa taaaaagatt aatttgttag tctactttac tgatggtaaa
    48721 ggtgaaaata aactacaagt aatacctaga gggtacaaaa ttttatgggt tatttctgga
    48781 agaggagata aaatttcatt aaaagaacct tatggagcag ttaaaaaact tactaaggtt
    48841 aaaacaaatg atgaaatatt agaaataaat gatgtaagaa atgatgggta ctcaatgaat
    48901 agccaagcac caattttata ggtattcaca ggagataagc tgtattaatt tattttgata
    48961 tgcttgttat aaaaatctaa aaaatacatt aatcaatggt taatgtattt tttaatttta
    49021 ataatataag ttttacttac atttaagtct caaaatgtgt taaaatataa tatatagcag
    49081 atagttttgg caaggtggat ttagttgtca aaactatttt ttttattaag gatggtgatg
    49141 tttttgttta agattgatga ttatataatg tatggtatga caggagtctg caaagtcatg
    49201 gatataacaa atgaacgatt tacaaatgga ataaaaaaag agtattatgt attgagtcct
    49261 atttattcta ataatacagt aataaaaatt cctgtagata atgaaaaagt accaatgaga
    49321 aagttacttt ctaaaattaa tgtgctttca ttaataaatg atattcctaa tatggataca
    49381 tcatggatag ataatgaaaa attgagaagt gagcaattta aaaaaatact cagaggtggt
    49441 aaatgtgaag agttaataaa attagtaaga agtatagata ataacagaga gtatgtaaaa
    49501 tctattggta aaaaaacaca tcaagcagat gataatataa tgaaagaagc tgaaaggtta
    49561 ttaaatgaag aatttgcaac tattttagat atttctccta atgaagttag ttcatatatt
    49621 tcaagtcata tacctcaata aagtgtttta gtatccttta tttaatctat tttaagtatt
    49681 atttatgtat gctggttttg gtaaaataaa atatataatt aaattatata aaaaataata
    49741 atgattgtat tgaagaatta cagaatagtg cggagagcct actcctttag gtgtgagatg
    49801 gatatcactt aaagatttaa attgttattg tagatgtttt taatataaaa caagattaat
    49861 aagtgtattg taagctttaa gtgaaattga tactataatg agttacttag attttaaaat
    49921 atataatatt ataaaaaatc cttttaggtt ttgaatgaat aagatggata ctatgttgaa
    49981 tagaatccat cttatttaag ttttatttat gttgtttatt tgtaagcatt atttttgtac
    50041 atgaaaacac agtgtaaaaa tatgcttaga attatgaagt tttattaaaa agatattcga
    50101 caaaataaga ctaaaaatat tcttaaactt ataactaaga taaatattgt atatttatag
    50161 tgtagcctga aaaattgtgg atgtacgaaa tttatttagt attttattga aacaaagtag
    50221 aagaagtact atagtttatc tttgaataga ttgttatttt ttagtgaaaa agttattagg
    50281 ttaatacata aatataatag cttataaata gtttaagcat gttaaaaaag tagttattag
    50341 agatatatag ttacattgct tataagatac ttaaaatgat agcattcact tatattcaga
    50401 agattaagat aaaaaatcat ttttata
//
LOCUS       DAMJVK010000018        48861 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.018, whole genome shotgun sequence.
ACCESSION   DAMJVK010000018 DAMJVK010000000
VERSION     DAMJVK010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 48861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48861
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.018"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            210..1427
                     /locus_tag="PC414_001039"
     CDS             210..1427
                     /locus_tag="PC414_001039"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HDF2463448.1"
                     /translation="MMNVTLTDVKEARETIKNIVKRTDLLESVKLSEKTGANVFYKCE
                     NLQKTGSFKLRGACNKIASLTDEEKANGVIASSAGNHAQGVALGAKMTGIKSTIVMPA
                     TAPLAKVSATKGYGAEVVLNGAVYDDAYAKAVEIQKETGATFLHPFNDKYVIAGQGTI
                     SLEIFEQLDNKVDTILCPVGGGGIISGVAVAAKALNPNVKIVGVQTANIPSMKESIKN
                     GKVTTAFNDTTIADGIAVKTPGDLTFEIINELVDEIVVVEETEIAESILFMMESQKIV
                     SEGAGAVCTAAILSGKYVPAKDENVVCIISGGNIDINTLYRIIGVALAKEGRRYSFST
                     IMEDKPGNFAELTRIISENGGNILSANQGKLSAGEALGKQSAEFILETIDYDHIARIK
                     KAIEEKGFKIIEL"
     gene            1522..1902
                     /locus_tag="PC414_001040"
     CDS             1522..1902
                     /locus_tag="PC414_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDF2463449.1"
                     /translation="MKHEVIHTNDAPAALGPYSQAIKAGNLLFVSGQVPLVPETMEVV
                     EGDVQAQTAQSLKNLKAILAESGADFSNVVKTTVFIKDMNEFGAINEVYAEYFGENKP
                     ARACVEVARLPKDVKVEIEVIAVL"
     gene            2186..2668
                     /locus_tag="PC414_001041"
     CDS             2186..2668
                     /locus_tag="PC414_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2463450.1"
                     /translation="MLNFFKKNKSYKLHAVVSGNSINIEKVNDSVFSKKLMGDGVAII
                     PNSNVVVAPCNGKVTVLTESKHAFGMVSDEGVEILVHIGIDTVSLQGEGFKNEVSQGD
                     TVKKGSPIISFEREKINSQGIDCTTIIIVLNHSEFSEINCMVENEVVAGQDTVIEIMK
                     "
     gene            2689..3504
                     /locus_tag="PC414_001042"
     CDS             2689..3504
                     /locus_tag="PC414_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2463451.1"
                     /translation="MRVVKIFNNNALSTVTNDRKEAILLGLGIGFNKRPGDKVNEDKI
                     EKIYYVQDHMQTKFLELLKNVSPEVMDASQQIISLIPGNEGKFNNKGILSLVEHISFA
                     IDRMKNNVFLPNLMLLDIKMMYSKEFELGVKALEIIYRVCHIHLPEDEAGYIALHFVN
                     LQSNDNLAYDTLKFVKGSIDLIKECYGLELDESSLSTLRFRTHLKFLAQRIFKNEICQ
                     DDKMIEMYDYLINNHPKNKEYLEKLNVYIEKEFRYKLDKPEKIYLLLHLTKIL"
     gene            3555..5105
                     /locus_tag="PC414_001043"
     CDS             3555..5105
                     /locus_tag="PC414_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2463452.1"
                     /translation="MKEKLLGGLETFAKAMVQPLMYLSSAGILMVVGVLLTNDMLLGV
                     LPFFKWEPIQIFGSLIYNCIMAIINNLAVIFAIGIPAALAKKEKHKAALIGFMSYIIY
                     LTASNTMLTSLGQLAKESKMLGLIGTGQATILGIQVVDTSVFGGIILGCIAGYVFNRT
                     SNKTFKGALQIYSGANFSFICMFFVSILIGVVSNFIWPLAQQGIASLANVIKDTGTFG
                     LFLYGFLERILVPTGLHHLIYTPFQFSDLGGVLKVGADTVSGAYAIVMTEMNLPVERF
                     SDSIYYMATGFTKMFGYIGIGAAFIYTAYKQNKKKVKATIIPLIITAVLASVTEPLDF
                     MFVFCAPFLFFVHAVIAGLFIALLKIFDVTALCGGNLLVSIIMNTTVGIEKTHWPVMM
                     ILGLAQIIVYFVVFSFLIKKFNYKTPGREDESTATGEEQSKQLNLDAGIENIIDGLGG
                     KENINTVENCITRLRVNVKDESKINEDIINLTPNSGIVRKGKDIQIIFGLHVHEVRRA
                     VEDFLEAY"
     gene            5202..6554
                     /locus_tag="PC414_001044"
     CDS             5202..6554
                     /locus_tag="PC414_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HDF2463453.1"
                     /translation="MIITIVGGGSTFTPGIVKSIALREKELELEEIRLFDIDKERQDK
                     VAVVVNWILKEDVKTDVKLTVTNCEKEAYTDATFIFAQMRVGKYEMREQDEKIPLKYG
                     CVGQETCGCGGMAYGLRTIQPMIKVIDDVEKYAKKDYWILNYSNPAAIVSEACRKLRP
                     NARIINICDMPIAIIDMIAGSLNINDVHNIRYDYFGLNHFGWFTSIDYKHRDLMEEIK
                     EYIKENKILLPKSYIENLNKSRSTGNRHAVSSWIHVWESVYTMLESFPKYLPNTYLTY
                     YLLSKETVEHSNINRTRANEVMESREKDLFEGVRHYLETGEISEKAFYAGSHGDWISD
                     LAVSIKNDTRSRFLVITENRGAIPNMPYDAMVEIPAYIGKDGPEVIARGPIPLFQQGL
                     MMQQLNSEKLIVEAYIENSYEKALQAFTLNKTIPSMNVAKEILDEMIEVNKEYWPELR
                     "
     gene            6685..7443
                     /locus_tag="PC414_001045"
     CDS             6685..7443
                     /locus_tag="PC414_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2463454.1"
                     /translation="MNIINKIFWEEDRIGMITNNLEADTHSHCMLQLFLGIEDSIEIT
                     VNEKLVKCNCIIVDKNISHSFSARKKVYYSAIIEPTSIYAEQLTSKMNDFGYWICDND
                     GLEKLRQQGTFLIDNSSKEQYLRFMEMLNNYLNIPITLKHYDDRITELLNLLRTCNCD
                     NHTISSFADKVSLSASRLSHLFKEQIGIPLKSYILFHQMECAFRELLSGKNVTEASMT
                     AGFDTPSHFAGTVKRMMGMPVSLSLKDSEFLKVY"
     gene            7490..8209
                     /locus_tag="PC414_001046"
     CDS             7490..8209
                     /locus_tag="PC414_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HDF2463455.1"
                     /translation="MNKEYLEETKMLNYNEPQLKLLVSSKNWLELDDFHKIKSIYEFV
                     QNDILFGYNASDMLSATQVLNDGMGQCNTKATLLMALLRAVNIPCRLHAFDVTKDFQR
                     GATSKLISLLAPKYILHTWVEVFYQDRWIALEGVITDKKYLEAIQKKFFNHGGTFKKY
                     AIATNDLKNTSIDWDGKDTFIQKEAIVYDYGIFPSPDVFFSTHSQHMSKLKNFIYVHL
                     IRKIMTKNVCKARNNYIDKNE"
     gene            complement(8333..9733)
                     /locus_tag="PC414_001047"
     CDS             complement(8333..9733)
                     /locus_tag="PC414_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDF2463456.1"
                     /translation="METQSNNKKGNLIIAIVMTGAFISSLSQTLLSTALPNIMSDFKI
                     TADIGQWLTTIYLLIAGIIVPTTAYLINRFSTRKIFITSMSIFSIGCIIALFSNNFST
                     MLIARVLQAMGSGSLMPLLQVIILYLCPEEKRGAAMSLVGITVGFAPAIGPTLSGWLV
                     DSFGWHSLFLFLSPIAILDVILSFILLRNVGETQKLKLDIPSIVLSSLGFGGLLIGFT
                     NQGNYGWTNIATYLPILIGIMSLILFTLRQLKSKEPFLELRVFKNKPFLISTILIMIV
                     YASMMSATLMIPLYVQSVRGFSALSSGSLMLPGAILMVVLNPIAGRHLDKYGPHALSI
                     LGTGCLFLGTLSFAFLGRDTSLIHVSLMYCIRMIGISMVLMPLTTWGIKTLDRELISH
                     ATAINNTLRQISGAIGSAILITIMTSATKKAHMSSNMLSNIHGIDVAFSIAATLAFTG
                     LIVSICFIKRHQIIRS"
     gene            complement(9737..10645)
                     /locus_tag="PC414_001048"
     CDS             complement(9737..10645)
                     /locus_tag="PC414_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2463457.1"
                     /translation="MKGDNKVTEKQRLIITIAQKIFDQKGFQNTSISDVVKECKMSKA
                     TFYKHFETKESFICEIINYYDEKFLEIIHSINENNDIPSSEKLNRKIIAVWKNIFSRT
                     TINTYIRENFSEEQRKATSKLQTKSRANLLNEYKLSLFDNYGDKIEKIIFDLVFLLDA
                     LIHQFIYIIHVQKREINVYFIAKFTIQILDLVVENMDNLNPLIEKSMFLHKQEDEVYF
                     SLHNKSLFFKTIQDIEELIKTDTSLLENPKLLEAIKKLYVEGKNQQYDSLIMDAMIAY
                     LEKEDTLRPKVLLLNSIKNQLKKETL"
     gene            10906..11553
                     /locus_tag="PC414_001049"
     CDS             10906..11553
                     /locus_tag="PC414_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDF2463458.1"
                     /translation="MKKKICVAIIFALFIVFVEMFIDSSSEGSKIMTAELSKYRKGVN
                     VLSINNIIVANKKYSLPKDYSPQESSEARDAFYKMNKDAQKSGLNLKAFSTYRSYEYQ
                     DRLFKSYVKEHGEKEANRFSAKPGESEHQTGLAFDIGGDDQSCWANKKFNNTKEAKWL
                     YENAYKYGFILRYPEGKENITGYMYESWHYRYVGTEHSKNFAMNNLTLEEYLHIN"
     gene            11878..14517
                     /locus_tag="PC414_001050"
     CDS             11878..14517
                     /locus_tag="PC414_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDF2463459.1"
                     /translation="MSYKSTYQEVIKNLDSSLSGLSKERAEELLEKNGANELKEADKV
                     PTYKLFLESFKDPLVIILLIAALVQIFLGETVESIIIFAVIIINSVLGVVQTKKAESS
                     LESLKNLSAPNAKVIRDNKKMTIPAKNLVVGDIVFLEAGDYIPADGRLIEAQSLKVVE
                     GMLTGESEPVLKHIEKISEDVSLGDQKNMVFSGAVVVYGRGSFVVTGTGMNTEMGKIA
                     GLLESAENKLTPLQQKIEDFSKKLGVGIVILALLIFIIQVARNYFMAGNGEMTQIILD
                     SFMFSVAVAVAAIPEALSSIVTIVLAVGTNSMAKKQAIIRKLPAVETLGSTSVICTDK
                     TGTLTQNKMTVVDYYMYGTNNDELDKEKVNYSYHAKLLTMVSTLCNDSHITSDGKEVG
                     DPTEVALINYSSKNDLDYDKLRSKYIRINEIPFDSDRKLMSTVNSIYGDYIMFTKGAP
                     DIVFSRCKYALKNGSKVDITDEIIEEYKHKNEEFSNRALRVLAFAIKDVPEEDFIPSI
                     DDEHDMTLVGIMAMIDPPREEVMDAVKEAKSAGIKTVMITGDHKTTAAAIAKEIGIME
                     EGDLALTGKELDALSEEQLYEKLENISVYARVSPENKIRIVKAWQHKNNITAMTGDGV
                     NDAPALKQADIGIGMGSGTDVAKDASAMVLVDDNFASIVNAVEVGRTVYSNIIKSITY
                     LFAGNLGAIVSIVFAVFAGWSNPFTALQLLFINLVNDSLPAIALGFEKSEKNIMNKPP
                     RHPDESILAGGTLKAVAIRGCIIGVVTIIAQYIGLQVSPELGVAMAFSTLILARIMQT
                     LPARSNNQTVIKLGFFSNKYVIGAVLVCLGLYSLTLIPVLRGIFSIPTTFGLYQLGIC
                     LGLALISSILMELNKLINRSEVK"
     gene            complement(14575..15678)
                     /locus_tag="PC414_001051"
     CDS             complement(14575..15678)
                     /locus_tag="PC414_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase family
                     protein"
                     /protein_id="HDF2463460.1"
                     /translation="MKLKINKAIEMQLKKSYAIESGHSEDVFEIDCGEGINTVSYSNK
                     AVEAFNALKFDMIRGYPHSIALKDNIVDYWKDFIALDTDRICLADGSIHVIYLLNRLF
                     IEKGDKVLGYSPQFSEYETDIKMHGATYDYVLLKKEDNFKFNEKEFIEKINPEYKVIY
                     IDNPNNPTGQIIPLSSIENIVREAAKYDIAVMVDEAYGEYMPKENSAVKLLNNYDNVI
                     ALKTFSKGFGLAGLRAGYAVLPEQLVSPIKKISTPYEVSEISRSIAANLLDDVQFIEE
                     LKEKTKDIKNQLLIPWKNLNIAETSDTVSIMTVEHKNKDIDLQQEFAKLKIRVISGSD
                     FTGLDKNFIRFRMPEEKELPEVIKAFQIIDNIE"
     gene            16038..16859
                     /locus_tag="PC414_001052"
     CDS             16038..16859
                     /locus_tag="PC414_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF2463461.1"
                     /translation="MGYKLIVTDMDGTLLGNNHKVTDENKTALQKVIKSGINVTLATG
                     RAFDSAKCNVDFLKEDMPIIACNGSLIREQNGNIIYSNKIDTRTCLNILDVLDKYDIY
                     YQCNSIDSMLSKKIEGREDRLSVFLGSETEVIVKDDLREEIFKKDILKFVIIEEKNPS
                     ILDEIRKELRKVQGIKITSSWPNNIEVMNEGVDKGNAVKILAEKMNIDREDIIAFGDN
                     YNDIEMIKFAGLGVAMGNAEELIKQEADYVTDTNQDSGVAKAIYKFLELKESLSS"
     misc_binding    16938..17149
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            17236..18552
                     /gene="argH"
                     /locus_tag="PC414_001053"
     CDS             17236..18552
                     /gene="argH"
                     /locus_tag="PC414_001053"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HDF2463462.1"
                     /translation="MKLWGGRFRKAENQLMEEFNKSFGYDCVLYKKDIEGSVAHVHMQ
                     VKCGLLTEEEGKSITEGLKGILEDVENGKLVLDGEYEDIHSFTEINLIQRIGDVGKKL
                     HTARSRNDQVAVDMRLYAKEKANDLVDLISEFKATIKDVADKNPVMMPGYTHLQRAQV
                     VTFKHHLMAYYSMFDRDEKRIKNAIEILDESPLGCGALAGTTHDIDRSITCEQLGFKK
                     VVDNFMDGVSDRDYLLELMSDFSIIMMHLSRLSEELILWSSQEFGFVEIDDLYTTGSS
                     IMPQKKNPDGAELIRGKTGRVYGNLFGLFTVMKGIPLAYNKDMQEDKEGFFDSVHTLE
                     MCIQIMDRMIATLKVNEDKMKQAVKNGFLNATEVADYLVKNNVAFRDAHGIVGSIVIY
                     CEDNKKAIEDLTLEELQKFSDAFKEDIYDFIDYESILNKGIKKNLK"
     gene            18801..19139
                     /locus_tag="PC414_001054"
     CDS             18801..19139
                     /locus_tag="PC414_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HDF2463463.1"
                     /translation="MNLLVIGGHERMEKDYMVMAKKKGYKTKVYTTMSSKLNNSIGKP
                     DAVVILTSTVSHKMSRTVESQAKKQDILIVRHKNSSKVAFNECLDMVDECLGNCSKCK
                     LKDKQKQKNI"
     gene            complement(19266..20555)
                     /locus_tag="PC414_001055"
     CDS             complement(19266..20555)
                     /locus_tag="PC414_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDF2463464.1"
                     /translation="MKRNLSLLLICLLIFTSFLGSSNISFADNEPAIVAKHAVLMDYE
                     SGKILYNKDGNSKLYPASTTKVWTACLVLKEVKDLNQVIEIKDLPQIDGSSMYLKNGE
                     SFTVKQLLDALLVHSANDAAFVLARYVGGGNVQKFIDLMNSEAKKIGATNTHFNNPHG
                     LPDPNHYTTAHDMALIAREAMNNDTFRQIVKTKSLKFEATKAYPYERYFVNTNKFLTS
                     HDKITYKGQPINIKYDIVDGIKTGYTDAAGKCLLSSAVKDGRRVIVAVFNSTNADLYL
                     DSRILIDYGFDNFKCATIVDKEKYTDTKKVLFTKQHELIYEPKNSYRIFLEKDESQGN
                     YDTKTELNKIDLPIKKGAKVGTLNVYNNGKLENSIDLIAKNNLDSSLPFLTENNVLMT
                     FVKIIAGILVLLVLFIIASNIKKKKRKKARSKKNMKK"
     gene            20635..21339
                     /locus_tag="PC414_001056"
     CDS             20635..21339
                     /locus_tag="PC414_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HDF2463465.1"
                     /translation="MKKKVKFISFFVLFILIISSIIVYKSLIFKDDIYVVSKKNLPNE
                     KTHVVENEIKGEDNNHSKVTKDIDKNFQNNKITIYISGAVNRPGIVTIESDKRLYDAV
                     ELLGGTTKEADLNGVNLSVRLEDEQHYIIPKIGEATSVTSNDDSNKLNQKNESRGEPK
                     NKSNKNISNESKVNINVATIEELDSLPGVGEATANKILQHREENGQFSSIEEIKNVNG
                     IGDKKYENIKDLICVD"
     gene            21431..22477
                     /gene="selD"
                     /locus_tag="PC414_001057"
     CDS             21431..22477
                     /gene="selD"
                     /locus_tag="PC414_001057"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:21479..21481,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HDF2463466.1"
                     /translation="MKKEQKKLTEMTTAGGUAAKIGPEVLASVLSQLPKNDNIENLLV
                     GLDTADDAAVYKLNDDMALIQTLDFFTPMVDDPYVFGQIAASNSLSDVYAMGGKPLVA
                     MNIVCFPSCHDMDVLAEILKGGFDKVKESGALLVGGHTVDDKEPKYGLSVSGIVSPNK
                     VLSNATAKPGDKLIITKPIGVGVLNTAMKEGMVEQHIADKVIEIMIHLNKYAAMSFEK
                     FDVNSVTDITGFGLLGHTLEMAKASEVSIEIESKHVPIIEGAIEMAQMGIIPAGMYKN
                     MHYVSKDVEVVGNIEVAVQDILYDPQTSGGLLISVKEELAEELVKDMKLNGAIEAKII
                     GSVVPKGEKYITVL"
     gene            22495..23907
                     /locus_tag="PC414_001058"
     CDS             22495..23907
                     /locus_tag="PC414_001058"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HDF2463467.1"
                     /translation="MIVLSKRELFAMLPSVDEVLSDNRIVEIINEYPRSLVLESVREV
                     IDLKRQFILRLKEDASNSVTIEFGEIIESAIERVKLNYSLSLKKVINATGTVIHTNLG
                     RSLLSEDIKDELWCAASRYSNLEYDLDNGERGSRYSHLTSTIKRLTGAEDVLVVNNNA
                     AAVLLVLSTMAKGGEAIVSRGELVEVGGSFRIPSIMALSGAELVEVGSTNKTHLKDYK
                     EAITEDTNVLMKVHTSNYRIMGFTESVSIEELVNLGKKYKLPVIEDLGSGVFIDLSKY
                     GLSYEPTVLDSIRQGADVVTFSGDKMLGGPQAGIIVGKKEYIEKMKKNQLTRALRVDK
                     LTICALEATLRMYLDETKAIENIPTLKMLTYKIEELEVKANKLFEKITALNLNANINI
                     EDGFSQVGGGSMPLETISTKVISITPEHMNVSSLEKKLRLSEAHIIARVYDNKYVLDV
                     RTIFDDEFDVIVEELRKAFN"
     gene            24021..25904
                     /gene="selB"
                     /locus_tag="PC414_001059"
     CDS             24021..25904
                     /gene="selB"
                     /locus_tag="PC414_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HDF2463468.1"
                     /translation="MKNVVIGTAGHIDHGKTTLIKALTGRETDTLDEEKKRGISINLG
                     FTYFDLPNNRRAGIVDVPGHEKFIKNMLAGASGIDIVLLVVAADEGVMPQTVEHLDIL
                     SFLNIKNGIVVLTKSDTVDDEFRELVKEDICEKIKGTFLEDAEIIEVDSISKRGIDTL
                     IEKIDKMSDDIEDKNENSPARLNIDRVFSIKGFGTVITGTLIEGKISIEDDLVIYPQV
                     LKTKIRSIQVHGENKDTAYAGQRTAINISNVKVDDIKRGDVLAAPNSLEESMMLDVKL
                     SLVNHLDKGLKHWDRLRLYHGTREILCRAVPLDKELIESGESGYVQLRLEESIVSKKG
                     DTFVVRRYSPMETIGGGVIIDPSPKKHKKFDEKVIEALKIKEKGELKDIIEEYLKRNL
                     KNYPNIKEIMSYSGAHEEDVKRALETLISEDKVFIIGNMYMHINQYNKLKENTIKLLS
                     EYHKKYRLRKGILKEEVRSKIESNFKTREMDILLEKLSTENAIKIENNIVSLLDFEVI
                     LNDKQKEIAKKIEKRLKSCGVSSILTIDEVSEGNHNYAEVLESMIGNKVEKLDDLYIM
                     DKDIYENAKNILINYIKENKEITLGEYRDLIDSSRKNCMIILENFDRNKITKRVENKR
                     ILF"
     gene            26009..28747
                     /locus_tag="PC414_001060"
     CDS             26009..28747
                     /locus_tag="PC414_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation-associated two-component system
                     sensor histidine kinase"
                     /protein_id="HDF2463469.1"
                     /translation="MKNIIPDVKPDIKEREDMSLLFIIMGSVLLLFIIKDIEKYETLL
                     HAIYTIFLVATGVIIFNTTKFKVDSFSTFLGLIFIATGVLECIYLFNSLGIKTKSIME
                     INITISAITDLFPILGVYLSFKFVKDNKQIYSSVILFIATATLTITSLFIISGICDYL
                     GNGKSQYALGVIISIFIIIISIISGIELNSSSNKGKWEYDEKKIFNRIIVIIILSRVP
                     NLLHIVIDNRNVERILSQIIINVALYYLYNYIVSKNIKKTALELHDTNEELTKKTESL
                     KEKNKKLIYETEKIEELKGILMKRESRLQSTLDVAVNSIVVFSKDGEITYANKSFRNT
                     FGDYKEQDKLTDKIKNFNDLIESIHYVFVHEKNAEKLISTSDNKVYQAIFAPLIIASQ
                     TEGVLCVLEDKTKKKEYERKLIEANKRSKDFLESIGDGIVVLEGNNKIYVNNACREIF
                     KNNLESIDFSLVCKSEESIEKRYVIDGEVKYVEMSFSQYTNAGTNKTIIVIRDTTSRK
                     ISQIKLEESQSSYSRFIDILPDGICLLKEDLSINYANKSFLDMLAFTNIDDIKDSNIK
                     LLMNANTEEKMKFTDNMAKVLNENRSILLLEHELINSYDDIVEVELSALPFAIYNTRY
                     IMLIIKDLVHKKYSEQAEKELLERFKTDKIKTEFFANMSHELKTPLNVISSSNQLVDS
                     FYRNEKIKDYNNNIKSHVDLVRQSSYRLQRLINNIIDLTKMESGFYTLKLAKYNIVSV
                     IEDLFMNIEEYALRKDIKILFDTDLEEINVYIDKVEIERIMLNLLSNCIKFTDNGGWI
                     YVSIHYKIDKVIISVKDTGVGIPQDKLELIFEEFSQVDKTLSRNTEGSGIGLAIVKNL
                     VSLHGGDIEVVSEVNKGTEFLISLPMKGFSSEHYTEDKRIYNIQEKIKIEFSDIYY"
     gene            complement(28866..29810)
                     /gene="manA"
                     /locus_tag="PC414_001061"
     CDS             complement(28866..29810)
                     /gene="manA"
                     /locus_tag="PC414_001061"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HDF2463470.1"
                     /translation="MEPLFLKPIFMDRIWGGTALKDKFNYEIDSPTTGECWAISSHKN
                     GDCLIENGKYKGKKLSELWNKNRELFGNTPGDKFPLLTKILDANDNLSVQVHPNDEYA
                     KKNENGELGKTECWYVIDCSDDAEIIIGHNAKSHKEFVNMVNNNEWDKLLRKVNIKKG
                     DFFYVPSGTIHAICKGTLILETQQNSDTTYRVYDYDRTDNSGNKRELHVQKSIDVTNV
                     PHINFDTDYKIVSTSDFKCTTFVSNEFFSVYKLDVFGKCNFTHNTPFSLYSVLDGNGK
                     LIHNSVEYNLKKGMHFILPNDFGDFSFEGNLEIICSHI"
     gene            29983..31344
                     /locus_tag="PC414_001062"
     CDS             29983..31344
                     /locus_tag="PC414_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2463471.1"
                     /translation="MELFKAESKKYKKEFLKLLEQWVSIPSFYDRKTVSKDMPFGKGV
                     YDALNWFENLGRENNFKVKNIDNHAVQIEYGNGKEYVDIFGHCDVVNPGEGWDSEPFK
                     LNIIGDKLVARGVSDNKGPMIVNFLALKMIKDLDINLKRKVRLIAGGNEESGFKCIKH
                     YYSKEPYGVCGFTPDAKFPVLNGEKGGAIIKLISNIDDKSLYISGGIEFNTIPDKVYI
                     KNVEKLGKDNICFDINNISINYDNGNYIVYGKGGHSSKPEKSINPILATIKLLSENID
                     EKWTKDLYKLINQDNINGNLFGLNIEGKCGILSMVPTIINIVDGKLEVVLSVRYPEIL
                     TIEDIIKKFNLYMEQNNINKFELIGENLKQANYIDRNSKLVRSLHDIYIKYSGDLKND
                     VRVTSAGSYASEMNNSVIFGCEFPDGSFGNVHSANEFASLDRFITAIGIYAEAIITLC
                     NKI"
     gene            31432..33321
                     /locus_tag="PC414_001063"
     CDS             31432..33321
                     /locus_tag="PC414_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2463472.1"
                     /translation="MITKKHAIIVKSLSNKDGYMTSNELAIKLDVSTKTIKRYIADLN
                     SVLSKYDLVIASSRGIGYKLSGSKNNIARAVKEANKYIDGFLDDSEESRMSNIICMLI
                     NRNYMSIEAMAEELNLSIAAINKLSSKLKRKLEKYDLVIKSKPYYGSYIHGEEINIRQ
                     LITDYAIKLDEKSKVKVFLDDISENDIHCIESILEKHLREKDTIISDKDFNLLLSKII
                     VSVFRSKRGHSNNINLMDTSYRFHNYYFIENLMKEISDKIGFKLIEDEVIYISNYSGV
                     IAYKGTQGRKNTSEIEERISSVISSALQDIFLISGSDYTKDDEFMVAISDHIKRFLNR
                     ARANVKSNNPLLHQIKEKFPIAFNLAVFLSNKLETEFNLKLDEDELGYIAIHFAASNE
                     RMKKNTSKKICIVCHYGIGTGQLISEKLKQNISDLSVVGVYPVRYLDMAISQDVDLIV
                     STVELKGYEKPVLYIENIFDDSLIENVNKAFYEKEERRKIISNMFDEKAFFSIKANTK
                     EEVIISLSNKLKERDFIEESSIKSIIDRENISSTEIGNLVAIPHTIVKGDKKSIIGVG
                     ILENPIIWDKQEVQLVFMVFFNTKEKHNFSIFKYLYNFIKDEGGVRGIIKICDFNKLM
                     ALIGN"
     gene            33336..33791
                     /locus_tag="PC414_001064"
     CDS             33336..33791
                     /locus_tag="PC414_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2463473.1"
                     /translation="MGTNIFNKEYVFLNVDAKSKVEVLKFISKKAKDLNLAEDESLVY
                     EGLMAREEQFTTNLGESIAIPHTKNDAIENPAVVVLKFNEDVVWNEGEDKVKLAISLL
                     MPGKSKENIHLKLLSSLSRKLINKEFKDSLLKSDNVEEISNLINEALGL"
     gene            33850..34167
                     /locus_tag="PC414_001065"
     CDS             33850..34167
                     /locus_tag="PC414_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HDF2463474.1"
                     /translation="MNKKLVALCACPMGLAHTFMAAEAIEQAAKALGYEAKVETQGAD
                     GVQNELTRDDILGATMIIHAVAITPEGMERFDGCEVYEVELQEAIKNAEGVIKEIEED
                     LGI"
     gene            34192..35454
                     /locus_tag="PC414_001066"
     CDS             34192..35454
                     /locus_tag="PC414_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDF2463475.1"
                     /translation="MAIKKKVINSSGASTTGGNNVGKSTPSSPTQSKGNGKWKEISKH
                     IMTGISYMIPVLVMGGLIGALSQLIPYAILGLDPSVGIVDAMNSGEFTGFKLSLLNIA
                     QLMSNFGFTLFGFAIPLFAAFCANSIGGKTALIAGFIGGYIANKPVGVPQFVDGQWTE
                     VVPVASGFLGAILIAFIIGYFVKWLNKSIKVSHNWLAFKTTFLIPLIASLACMVLMIF
                     IITPFGGLINESMKNFLTAAGAAGEYVYATALAAATAFDLGGPINKAAGFVALGLTTE
                     NVLPITARTIAIVVPPIGLGLTTLLDKRLVGRRVYDRQFYQAGKTSIFLSFMGISEGA
                     IPFALERPGFVIPLNIVGSVIGAITGIILGAIQWFPESAIWAWPLVDNLFGYIIGIAV
                     GAIFIAVGNIFYRNKLIKDGKLVVDYID"
     gene            35559..36623
                     /locus_tag="PC414_001067"
     CDS             35559..36623
                     /locus_tag="PC414_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF2463476.1"
                     /translation="MDKIEKVREYFREYDIDGFLINSPTNKFYVGNLFSSSGYVFITK
                     ESQYIIVDFRYFEEIKRKSSLFNVVLMDKTRTHFDIINDICREQNIKEIGFEGNEVSF
                     DLYRSMSNKLSATLKSVDLSTLRETKNEDEIKYIKKACEIVDATFYHIVDFIKVGMTE
                     KQVENEIVRIIKDLGGQKESFDTIVASGLRGALPHGKASEKVIEYGDFVTFDFGAKYN
                     NYCSDITRTICMGTINKELEEIYNIVRKANEECIRVLRPGMTTGEIDKVARDIIGSYG
                     YAGNFGHNLGHGVGIMVHEYPALAPESNEVLKEGMIVTIEPGIYVPSLGGVRIEDDVL
                     ITQDGCMRLTTSTKDLIVVS"
     gene            36677..37855
                     /locus_tag="PC414_001068"
     CDS             36677..37855
                     /locus_tag="PC414_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HDF2463477.1"
                     /translation="MYNFDDKPDRVSEKCRKWDLNIIRDKFGDIREDFIPMWIADMDF
                     KIPTEIENKFIEAVRRGVFGYTYCYDEFYDSVIGWQKNMHEVNVEKEWITLTYGTVST
                     LHYVVQAFCKKGDSIILNTPVYDPFESSAKKQGVNVICNTLDVVNDRYYINFDKLEVQ
                     IKENKPKLMMFCTPHNPSGRIWTIEEMTRVATICKENNVILVADEVHAEHIHYGTFNS
                     ILKIGKELLENVILLTSPNKGFNLGGLKTSYSIVINKDIRDKFRNKLKQNSITSPNVF
                     GIIGLITAYNECHEWLSGVNEYIKSNYELLETWVNKYNKIKLMKMESSYLAWMDISGL
                     GISASEFTDKLAIDTGVLLEDGSHFVKDGEKYVRINLGTQKENVIEALNRMDLFLKSL
                     "
     gene            complement(38046..39104)
                     /locus_tag="PC414_001069"
     CDS             complement(38046..39104)
                     /locus_tag="PC414_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HDF2463478.1"
                     /translation="MNKKKITFIFVILILALILFSFFRSSSQNTKYSKTSYYLGTVNE
                     VTVFNVKESKSDKILNECDSILRYIDNKMSTHIPGSDVSKINDNAGKKFVRVSDDTFF
                     VVKEAIQYSKLSDGYFDITIGPLSNLWAIGTDKAKVPSDSEIQKLLPLIDYKNIILDE
                     KNKSIKLTKENMKIDLGAIAKGYAADKIVAYLKSEDVKKGLVNLGGNIFTLENGKNDK
                     PFKIGIQDPTSKNGESIGNIETTNKSVVTSGIYERFIEKNGKIYHHMLNPFTGYPFEN
                     NLSSVTIISDKSINCDALSTSTFGLGLEKGMNLINKIDNVDAIFITKDKKVYLTKGIK
                     DNFKLTDKSFSIEKLNKQ"
     gene            39171..39542
                     /locus_tag="PC414_001070"
     CDS             39171..39542
                     /locus_tag="PC414_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HDF2463479.1"
                     /translation="MKKKDILLILGILVVITACYGIINVINSKNAGNIEIYVDNKLYK
                     TVSINAKEEFKIENRGGYNIVKIHDKGVEIVDASCPDKVCVHTGFINKPSQSIVCIPN
                     RVSIKINTNEKNDNQEDIISK"
     gene            39579..40253
                     /locus_tag="PC414_001071"
     CDS             39579..40253
                     /locus_tag="PC414_001071"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDF2463480.1"
                     /translation="MVNLGNDWDELLKEEFEKDYYLNLRKFLIEEYKTRQIFPNMHNI
                     YEALKHTSYKDTKVLILGQDPYHGDNQAHGLAFSVQPQVKTPPSLLNMYKELKDDLGC
                     FIPNNGYLMPWADQGVLLLNTALTVRAHEANSHKNKGWEIFTDRVISILSEREDPVIF
                     VLWGSNARKKVELIDTSKHYILEAPHPSPLSASKGFFGCKHFSKINEILKKLGKEPIN
                     WQIENI"
     gene            40502..41341
                     /locus_tag="PC414_001072"
     CDS             40502..41341
                     /locus_tag="PC414_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HDF2463481.1"
                     /translation="MNFKKLSNAKVSVMAVVLSVGILSGYSALNKQVTLVVRGEERTI
                     STFSSNVGDLLESQNISYDANDIVTLDENTKLSNGDKIEVIDVKEKTVVETKDIPFEV
                     TVVEDGSLLKGDSKVKEEGKSGKSELIYKETYHNGKKVEKEFVKKVVKAEPVNKVVSK
                     GTKVEMQVASSRGESSRRMASSSSSSSSSNNGKNMRVVATAYTGHSITATGTKPKWGT
                     IAVDPKVIPYGTKVYIPQFDMVFTAEDCGGAIKGNKIDIYMNDSKTVYNWGRKTIDIR
                     ILK"
     gene            41585..41681
                     /locus_tag="PC414_001073"
     tRNA            41585..41681
                     /locus_tag="PC414_001073"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:41619..41621,aa:Sec,seq:tca)
     gene            41831..42538
                     /locus_tag="PC414_001074"
     CDS             41831..42538
                     /locus_tag="PC414_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HDF2463482.1"
                     /translation="MENNFTTRTSFLVGDDGIEKLNNSNIIVFGVGGVGSFTVEALAR
                     AGVGNITIVDFDDVDITNINRQIPALHSTVGRYKVDVMEERILDINPNINIKKIRSLY
                     NKDTSDEILTERYDYVVDAIDMVSSKIHLIETCEKKGLKIISSMGMGNKLDPTKIVVT
                     DIHKTSTCPLAKVMRKELRDRGIKKLKVVYSTEQPIELKKKVMNGRKVTPGSVSFVPS
                     VGGLIIASVIVNELLGQ"
     gene            complement(42680..43516)
                     /locus_tag="PC414_001075"
     CDS             complement(42680..43516)
                     /locus_tag="PC414_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2463483.1"
                     /translation="MRNKIFLSILIIISLLIGCDKKSLLSIHMIDVGQGDSILVQTPT
                     NKNILIDGGDEDSENIIISYLRQKRIKTIDIIIATHPDSDHIGSLDNIIKKFNVNSIY
                     MPEQSTDSEAYQNLINSCTDKNLSIQHLYKNDVLNIDNNINIYVLSPSYIQEESNLNS
                     IVFKLTFNDNSFLFMGDAEEENEKEILHSFKLNNINFIKIGHHGSNSSSSLEFIKKIS
                     PDIAAISCGYKNQYGHPHREVINNLKQNHVSIYRTDRIGDIVFYSDGEIIFTKYNYEI
                     DT"
     gene            43704..44609
                     /locus_tag="PC414_001076"
     CDS             43704..44609
                     /locus_tag="PC414_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2156 domain-containing protein"
                     /protein_id="HDF2463484.1"
                     /translation="MFFKDIELNSKKELDPYFDLVDYEACEYCFSTLYMWQHVYKTGY
                     YIGEDFAVLVGEYEGDSFSILPLAKKDKLPEVVDFVLEYFSKNNKKIYLRGITTEVVE
                     FLKEKYPGRFEYIEERDLFDYIYDAESLRTLAGKKNQKKRNHINYFLKEYAGRYEAKL
                     LDKENFDECLVLMKEWESNKEENNEFDESMDDELIGIKKIFNHYDILKDKVKVFGVYV
                     DGKLEAFSIGELLNPNMALIHIEKANPDIRGLYPFINQQFLVSEFKDVEFVNREEDLG
                     IEGLRKAKLSYHPCRFVEKYSVREA"
     gene            44618..45802
                     /locus_tag="PC414_001077"
     CDS             44618..45802
                     /locus_tag="PC414_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2463485.1"
                     /translation="MEIRYAKEEEIESIKEIWSYCFNDTESFMKYYFNDKYKSENTVV
                     ALDEGKIISSLQLNQYKLLLNSKVYNTSYVVGVSTLPEGRGAGYMNKVMKFTLNELYK
                     KGQLVSILMPIDYRLYRRFGYEHCYDQIEYTINTDDLKNFKSSGKMIKSNLSQIDDLI
                     QIDRTFLNEVNGNVLKDEHYYENLFKEIQSEDGFLYIHEGNEKDGYIVYFLQEDKMFV
                     RELFYKNIDALKSMLKFIYNHNTQCKIVTISTPTIDKIRFILDNPKDSDIKIKPFMMG
                     RVINVKKFIEDIDIENDINSSFNLLIEDKFIDENNGLFKISIQNKKVSVEQLDKKGAE
                     KPQEDFDIKLDINTLTQLSFSYIDVNEAIFLNDIKDVSEETLETLNCIFSKKNNYINE
                     YI"
     gene            45917..47218
                     /locus_tag="PC414_001078"
     CDS             45917..47218
                     /locus_tag="PC414_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HDF2463486.1"
                     /translation="MGVIIIQIRRPLLIILMFVVAISFIITNNTKNDEELNDKIITIQ
                     GVVKGKIEKKRYNQYKVGVFLINDYTKNKNLKIGQKVNITGKFKSLDKMKYDDFDYGR
                     YIKSTGYKGIVYINNYKIIDKNKLYSLIGEIKFYISKTYRYLYKKNSDFINSILLAEV
                     ENLTEEQKEIFTRTGTSHVISISGLHTGILCVIISFLLRGINKLYKLLILSIFITLYC
                     IMVGASPSIIRSIAFVMIFYLSIFIDRKKDGISALSLIGIILIMNNPYVIYNVSFQLS
                     FLATLSILIFYNKINSIIKLSMVSLTISSNILTLPIIYYTFKGIPLLSIIGNLIIVPF
                     VGVIMYLSIASLIVFKVSVVIAKIISFFNSTFIESIFFLLEKISNLSFAYINIENPKF
                     YIVVIYYIGVFFYIFYIEGKEIKEQENELQGYYKECKREKL"
     gene            47172..48215
                     /gene="holA"
                     /locus_tag="PC414_001079"
     CDS             47172..48215
                     /gene="holA"
                     /locus_tag="PC414_001079"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDF2463487.1"
                     /translation="MNYKDIIRNVKEKNFEKMYLFYGREYYLIENAIKAFKDSLNEGM
                     LDFNLDIIDGKEIVLNQLISSIETLPFMDDRKIVIIKDFELLKGKKKNFTDSDEKYLI
                     EHLDNIPDTTTIVFVVYGDVDKRKSLVKKIGNNGIVFDCDKLSDMDLFKWIKKSFALN
                     DVIIDNSQIMYFIEQEGYRDKSSEKTLSDLENEINKISSFVGKGNNVTNDVIDKLSQK
                     KVENDIFKLIDYIGEQNASNAMKILNDMIQEGESVLGIFSMIARQFKIIMQVRQLQLD
                     GYSTKLIADKLKMHQFVVGKALKQTKNFSDDIIVEILNYILESDYKIKTGLIRDTLAV
                     EMLVSRYCKREAI"
     gene            complement(48402..48668)
                     /gene="rpsT"
                     /locus_tag="PC414_001080"
     CDS             complement(48402..48668)
                     /gene="rpsT"
                     /locus_tag="PC414_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HDF2463488.1"
                     /translation="MANIKSAKKRISVIEKKTALNRVRKSQIKTAIRRFEDAVAAGNR
                     EDAVAKFQYAQKRIYQVASKGTIHKNAAARKVAKLAQKLNGMNA"
BASE COUNT        19166 a         5269 c         8360 g        16066 t
ORIGIN      
        1 aatgtaaaaa atatttttaa attatcattt aatgtaattt aaatgaattg aagatatatg
       61 tttattgtat gcaattgttg tatttttagg ttttttgtga ttaattaaaa aattataaaa
      121 atcgtttaca tgttgatttt taaaaaaaaa agatatagaa ttatatagaa agtaataaac
      181 aaaaattgga tttacgggag tggaatgaaa tgatgaatgt taccttaaca gatgtgaaag
      241 aggctagaga gaccattaag aacatagtaa aaagaactga cttacttgaa agtgttaaac
      301 ttagcgaaaa aacaggagca aatgtgttct ataaatgtga gaatcttcaa aaaacaggtt
      361 cttttaaatt aagaggagct tgtaacaaaa tagcgagttt aacagatgaa gaaaaggcaa
      421 atggagttat agcttcaagt gcagggaatc atgctcaagg agttgcattg ggagcaaaaa
      481 tgactggtat aaaatctaca atagtaatgc cagcaactgc gcctttagca aaagttagtg
      541 caactaaagg ttatggagca gaagtagttt taaatggtgc ggtttatgat gatgcatatg
      601 caaaagctgt tgaaatacaa aaagaaacag gagcaacttt cttacatcca ttcaatgata
      661 agtatgttat agcaggacaa ggaactatat ctcttgaaat tttcgaacaa ttagacaata
      721 aagttgatac tatactatgc ccagttggtg gaggaggaat catctctggt gttgctgttg
      781 cagcaaaagc attaaatcct aatgtaaaaa tagtcggtgt tcaaactgca aatatacctt
      841 caatgaaaga gtcaataaaa aatggaaaag taacaacagc atttaatgat actacaatag
      901 ctgatggtat agctgttaaa actccaggag atttaacttt tgaaataata aatgaattgg
      961 ttgatgaaat agtagtggtt gaagagacag aaattgcaga atcaatatta tttatgatgg
     1021 aaagtcaaaa aatagtttca gaaggtgctg gtgctgtatg tacagctgct atattaagtg
     1081 gtaagtatgt accagcaaaa gatgaaaatg tagtttgtat aatatctggt ggtaatatag
     1141 acataaatac gttatataga ataataggtg ttgctttagc taaagaagga agaagatatt
     1201 catttagtac aattatggaa gataagccag gaaactttgc tgagttaact agaataataa
     1261 gtgagaatgg tggaaacatt ttaagtgcta atcaaggtaa attatcagca ggggaagctc
     1321 taggaaaaca aagtgcggaa tttatattag agacaataga ttatgaccat atagctagaa
     1381 taaagaaagc tatagaagaa aaaggtttta aaattataga attataataa aaagtgtata
     1441 taatatacaa gaaaaatagt ataatagtgt atgaagatac attttatgtg tattagatta
     1501 acgaattagg aggattttaa aatgaaacat gaagtaatac atactaatga tgcaccagca
     1561 gctttaggac catattctca agctataaaa gctggaaact tgttatttgt atctggacaa
     1621 gttcctcttg taccagagac tatggaagta gttgaaggag atgtacaagc tcaaactgct
     1681 caatctttaa aaaatcttaa agctatatta gctgaaagtg gagctgattt ttcaaatgta
     1741 gttaagacta cagtatttat aaaagatatg aatgaatttg gggctataaa cgaagtttat
     1801 gctgaatatt ttggagaaaa taaaccagca agagcttgtg tagaagttgc tagattacca
     1861 aaagacgtta aagttgaaat agaagtaata gctgtattat aaaaatacaa tatttttaat
     1921 ttgaaaaggc tgaaaaacta tattctagtg attcagcctt tttataaaaa aataaatcac
     1981 aataaagaac atgataaata ttgtcgaatt aaaaaataaa attaaaataa atattgcaat
     2041 atttttgaga aggttttata atgagtatat aatttgaaac gtgtaactat taaattaggc
     2101 atgagttctt ggatattttt atcctgagga ttcatgcctt ttttgtattg aaattcaaaa
     2161 atctatttaa taaggaggaa gtactgtgtt aaattttttc aagaaaaata aaagctataa
     2221 gttacatgca gtagtgagtg gtaacagcat taatattgag aaagtaaacg attctgtgtt
     2281 ttcaaaaaag ttaatgggag atggagttgc aattattcct aatagtaatg ttgttgtagc
     2341 cccttgtaat ggtaaagtaa ctgtgcttac agaatcaaag catgcctttg gtatggtctc
     2401 agatgagggt gttgaaatat tagtccacat aggaattgat acagttagtt tacagggtga
     2461 ggggtttaaa aatgaagtgt ctcaaggaga tactgttaaa aaaggtagtc caataataag
     2521 ttttgaaaga gaaaaaataa attctcaggg aattgattgt acaacaataa taattgtatt
     2581 aaatcatagc gaattttcag aaataaattg catggtagaa aatgaagttg ttgcagggca
     2641 agatactgtt attgaaatta tgaaataata ggaagggatt aattcaatat gagagtagta
     2701 aaaatcttta ataataatgc attatctaca gtaactaatg ataggaaaga agctatttta
     2761 ctaggtcttg gtataggttt taataaacgt ccaggagata aagttaatga agataaaata
     2821 gaaaagattt attatgttca agaccacatg caaacaaaat ttttagaatt attaaaaaat
     2881 gttagtccag aagtgatgga tgcttcacaa caaattataa gtcttatacc aggaaatgag
     2941 ggaaaattta ataataaagg gatattatct ttagtggaac atattagttt tgcaatagat
     3001 agaatgaaaa ataatgtttt tttacctaat ttaatgcttt tggatataaa gatgatgtat
     3061 tcaaaagagt ttgaattagg agtaaaagct ttagaaatta tatatagagt atgtcatatc
     3121 catttgcctg aagatgaagc aggttacata gctttacatt ttgtcaattt acaatcaaat
     3181 gataatttgg catatgatac tttaaaattt gtaaaaggta gtattgattt gattaaagaa
     3241 tgttatggat tagaattaga cgaatcgagt ttaagtacat tacgttttag aactcatctt
     3301 aaatttcttg ctcaaagaat ttttaagaat gaaatttgtc aggatgataa aatgattgaa
     3361 atgtatgatt atttaattaa caatcatcct aaaaataaag aatatttaga aaagttaaat
     3421 gtatatattg aaaaggagtt taggtataaa ttagataaac ctgaaaaaat ttatctacta
     3481 ctacacctaa caaaaatctt ataagaatga gatgaattag taatagaaat atataatttt
     3541 agggggggat aagattgaaa gaaaaactac ttggaggact ggaaacattt gcaaaggcaa
     3601 tggttcagcc attaatgtat ttatcgagtg caggaatatt aatggtggta ggtgtactac
     3661 ttacaaatga tatgttatta ggagtacttc cattttttaa atgggagcca attcaaatat
     3721 ttggaagcct aatttataac tgtataatgg ctattattaa taatcttgct gttatatttg
     3781 ctataggtat tcctgctgca ctagcaaaaa aagagaagca caaagcagcg ttaataggat
     3841 ttatgagtta tataatatac cttacagctt ccaacactat gttaacatca ctaggtcaac
     3901 tagcaaaaga aagcaagatg cttggactga tagggactgg acaagctact attttaggta
     3961 ttcaagttgt agatacaagt gtatttggtg gaattatttt agggtgtata gctggatatg
     4021 tatttaatcg tacaagcaac aaaacattta aaggagcttt acagatatac tcgggagcaa
     4081 attttagttt tatatgcatg ttctttgtat ccattttaat tggagttgtg agtaacttta
     4141 tatggccatt agctcaacaa ggaatagctt ctcttgcaaa tgtcatcaaa gatacaggta
     4201 catttggttt attcttatat ggttttttag agagaatatt agtaccaact ggacttcatc
     4261 atttaatcta tacaccattt cagttttctg atttaggagg agtgcttaaa gttggtgcag
     4321 atacagtttc aggagcctat gctatagtta tgacagaaat gaatttacca gttgagcgtt
     4381 tctcagatag tatatactac atggcaactg gatttacaaa gatgtttggt tatataggta
     4441 taggagctgc ctttatttat actgcatata agcaaaataa gaagaaagtt aaggcaacga
     4501 ttattccact aataattacg gcagtattag cctctgtaac agaaccatta gactttatgt
     4561 ttgtattctg tgcaccattt ttattctttg tacatgcagt tatagctggt ctttttatag
     4621 ctttattaaa aatatttgat gttactgcat tatgtggagg gaacttatta gtatcaatta
     4681 taatgaatac tacagtaggc atagaaaaaa cacactggcc agtaatgatg atattaggac
     4741 ttgcacaaat tattgtatat tttgtagtat ttagtttttt aattaagaaa tttaattata
     4801 aaactccagg tcgtgaagat gagagtacag ctacaggcga agaacaatca aaacaactta
     4861 atttagatgc agggatagaa aatataattg atgggttagg cggaaaagaa aatattaata
     4921 cagtagaaaa ttgcatcact agattacgtg ttaatgtaaa agatgaaagt aagattaatg
     4981 aagatataat taatttaacg ccaaatagcg ggattgtaag gaaaggaaaa gatatacaaa
     5041 taatttttgg acttcatgtg catgaggtaa gacgtgcagt agaagatttt ttagaagctt
     5101 attaaagata tatatttata tagcaagggt aaataacagc tttaagtgct gtttactatt
     5161 tattaaagat acatataatt tatagaggag gaataattat tatgattatt actattgttg
     5221 gtggaggaag cacatttact ccaggtattg ttaagtcaat tgcattaaga gaaaaagagt
     5281 tagagcttga ggaaattcgc ttatttgaca ttgataaaga acgccaagat aaagtagctg
     5341 tagttgtcaa ctggatacta aaagaagatg ttaaaacaga tgttaaacta actgtaacta
     5401 attgtgaaaa agaagcgtat acagatgcta catttatatt tgcacaaatg cgtgttggta
     5461 aatatgaaat gcgtgagcag gatgaaaaaa ttccattgaa atatggttgt gtaggtcaag
     5521 aaacttgtgg ttgtggaggg atggcatacg gactgcgtac aatacaacca atgataaagg
     5581 ttattgatga tgtagagaaa tatgctaaaa aagattattg gatattaaat tactctaatc
     5641 ctgctgcaat cgtttctgaa gcttgtcgta agttaagacc aaatgcaagg attataaata
     5701 tctgtgatat gccaatagct attatagata tgatagcggg gtcattaaat atcaatgacg
     5761 ttcataatat acgctatgat tattttggtc taaatcattt tggctggttt acttcaatag
     5821 attataaaca tagagactta atggaagaga ttaaggaata cataaaagaa aataaaatat
     5881 tattaccaaa atcttatata gaaaacttaa ataaaagtcg tagtacagga aatcgccatg
     5941 ctgtaagtag ctggatacat gtttgggaat ctgtttatac aatgttagaa agcttcccaa
     6001 aatatctgcc aaacacttat ttaacatact atcttttaag taaagaaaca gtggaacatt
     6061 caaacataaa tcgtactcgt gcaaatgagg ttatggaaag ccgtgaaaaa gacttatttg
     6121 aaggtgtgag acattactta gaaactggag aaatttctga aaaagcattt tatgcaggtt
     6181 cacatggaga ttggattagt gatttggcag tttcaattaa aaatgataca cgtagtcgtt
     6241 ttttagttat aactgaaaat cgtggagcta taccaaacat gccatatgat gcaatggtag
     6301 aaattccagc atatattggt aaagatggtc cagaagttat tgctcgaggt ccaatacctc
     6361 tatttcaaca aggattaatg atgcaacaac ttaacagtga aaaattgata gtagaagctt
     6421 atatagaaaa tagttatgaa aaagctttac aagcattcac attaaataaa actattccaa
     6481 gtatgaatgt tgctaaagaa attttggatg aaatgataga agttaataag gaatactggc
     6541 cagaattaag atagtaggat aaaggaaagg gtttttattt aataattaga tatgttttca
     6601 gagtctttta tcaaaatgta ttcacaaaca tatggcaata tattaaaata aagagtgata
     6661 tttttagaag aatggggtga tgcaatgaac attataaata aaattttttg ggaagaagat
     6721 aggattggca tgattacaaa taatttagag gcagatacac atagccactg tatgttacaa
     6781 ctgttcttag ggatagaaga cagcattgaa ataacagtca atgaaaaatt agttaaatgt
     6841 aattgtatca tagtagataa aaatatctct cattcatttt cagcaagaaa aaaagtttat
     6901 tactctgcta ttattgaacc tacatctata tatgcagaac agcttacctc aaaaatgaat
     6961 gattttggct actggatatg tgacaatgat ggattagaga aattacgtca acaaggaact
     7021 tttttgatag ataactctag taaagaacaa tatttaagat ttatggaaat gcttaataac
     7081 tatttgaata tacctataac tctcaaacat tatgatgaca gaattactga attgctcaat
     7141 ttattacgta cttgtaattg tgataatcat actatttcaa gttttgcaga taaagtgtcc
     7201 ctatcagcaa gtagattatc acatctgttt aaagaacaaa tagggatacc tttaaaaagc
     7261 tatatcttat ttcatcaaat ggagtgtgct tttagagaat tactttctgg gaaaaatgtt
     7321 accgaagctt ctatgactgc tggattcgat actccatcac actttgcagg gacagttaag
     7381 cgaatgatgg gaatgcctgt ttctttatct ttgaaagata gcgaattttt gaaagtatat
     7441 taaatattct cgttatataa tgaagttgta ctaagaaagg ggctttgtta tgaataaaga
     7501 atatttagag gaaacaaaaa tgttaaatta taatgaacca caacttaagc ttttggtatc
     7561 atcaaagaat tggttggaat tagatgattt tcataaaata aaatctattt atgagtttgt
     7621 acagaatgat attttatttg gatataatgc ttctgatatg ttaagcgcaa cacaggtatt
     7681 aaatgatggt atgggacaat gcaatacaaa agctactctt ttaatggcat tattacgtgc
     7741 agtgaatatc ccttgtaggc ttcatgcttt tgatgtaaca aaagattttc aacgaggagc
     7801 tacatccaaa ttgatttcac tattagcacc aaaatatatt cttcatactt gggtagaggt
     7861 attttatcaa gataggtgga ttgctcttga gggtgtcata actgataaaa aatatttaga
     7921 agcaattcag aagaaatttt ttaatcatgg tggaacattt aaaaagtatg caatagctac
     7981 aaatgatttg aaaaatacat ctattgattg ggatggtaaa gatactttta tccaaaaaga
     8041 agctatcgtg tatgattatg gtatttttcc atcaccagac gtattttttt ctactcattc
     8101 acaacatatg tcaaaattaa aaaatttcat atatgtacat cttatcagaa aaataatgac
     8161 aaaaaatgta tgtaaggcta gaaacaatta tattgataaa aacgaatgag tgtttaagag
     8221 gtaactatat aggaaatata tagaataatt aaaactgtac tggttaaaat gaataaaaag
     8281 gagttatcac agagcaaact taaattgttc ataggataac tccttttctg tattaacttc
     8341 taataatttg atgtcttttt ataaaacata tagatacaat taaaccagta aatgcaagag
     8401 tagctgctat tgaaaatgca acatctattc catgaatgtt agacagcatg tttgaactca
     8461 tatgagcttt cttagtagca cttgtcatta tagtaataag tatagcagaa cctatagctc
     8521 cagatatctg acggagtgtg ttattaattg ctgtggcatg agatataagt tctctatcta
     8581 atgttttaat tccccatgta gttaatggca ttaaaaccat tgaaatacca atcatacgaa
     8641 tacaatacat caatgacaca tgaattaaag atgtatctct gcctaaaaaa gcgaaggata
     8701 aagttcctaa aaacaaacat cctgttccta atattgatag agcatgaggt ccatatttat
     8761 ctaaatgacg tcctgcaatt ggattgagta caaccatcaa tatagcacca ggtaacatta
     8821 atgaacctga agataatgca gaaaatcctc ttacagattg aacgtacaga ggaatcatta
     8881 atgtagcaga catcattgag gcatatacaa tcataattaa tattgtagaa ataagaaatg
     8941 gtttattttt aaaaaccctc aattctaaaa aaggttcttt agattttaat tgacgcaatg
     9001 taaataaaat taatgacata attcctatta atattggtaa atatgtggca atattagtcc
     9061 acccataatt accttgatta gtaaatccaa ttaataaacc accaaaacct agagatgata
     9121 gtactattga aggtatatct aattttagtt tttgagtttc cccaacattt cttaatagaa
     9181 taaatgatag tataacatct aatatagcaa taggggaaag aaacagaaat aaactatgcc
     9241 aaccaaatga atctactaac catcctgata aagttggacc tattgctgga gcaaatccta
     9301 ctgtaatacc aactaaactc atagctgcac cacgtttttc ctcagggcat aaatagagta
     9361 taataacttg taaaagtggc attagagaac cagaacccat tgcctgcaac acacgagcaa
     9421 tcaacatagt tgaaaaatta ttagaaaaaa gtgcaataat acatcctata gaaaagatag
     9481 acattgatgt aataaatatc tttctagtac taaatcgatt tattaaataa gctgttgtgg
     9541 gtacaataat acctgcaata agtagataaa ttgttgttaa ccactgacca atatcagcag
     9601 taattttaaa atctgacatt atattaggta atgctgttga caatagtgtt tgactgagag
     9661 aacttataaa agcaccagtc attacaattg ctattattaa atttcctttt ttattgttac
     9721 tttgggtttc cataatttat aacgtctcct tttttaattg atttttaatg ctgttcaata
     9781 aaagtacttt tggtcttaaa gtgtcttctt tttctaaata tgcaatcatt gcatccataa
     9841 ttaaagaatc atattgttga ttttttcctt cgacgtataa tttttttatt gcttctagta
     9901 atttaggatt ttcaaggaga gaagtatcag ttttgattag ttcttctata tcttgtatcg
     9961 ttttaaaaaa tagcgattta ttatgaaggg aaaagtaaac ttcatcttct tgtttatgta
    10021 aaaacattga tttttcaatt aaaggattta gattatccat attttctaca actaaatcta
    10081 gaatttgtat agtaaatttg gcaataaagt atacatttat ttctcttttt tgtacatgaa
    10141 taatgtatat aaactgatga attagagcat ccaataagaa aactaaatca aatataattt
    10201 tttctatttt atcaccatag ttatcaaata agcttagttt gtattcattc aataagttag
    10261 ctcttgattt tgtttgcaat ttacttgtag ccttacgttg ctcttcagaa aagttttctc
    10321 ttatataggt atttatggta gttctagaaa aaatattttt ccatacagca attatctttc
    10381 tgtttaactt ttctgatgat ggaatgtcat tattttcatt tatggaatgt ataatttcta
    10441 aaaacttttc atcataataa ttgattattt cgcagataaa actttcctta gtttcaaaat
    10501 gcttgtaaaa agtagcttta gacattttac actcttttac aacatccgat attgaagtat
    10561 tttgaaaacc tttttggtca aaaatttttt gtgcaatagt tataatgagc ctttgttttt
    10621 ctgtcacttt attatctcct ttcacgcaaa aacgtttagt actatatagt acctacaagt
    10681 tttgataaat atattataca tcatttggaa aaaaaatcaa tatatagact gtaatatgaa
    10741 atttttgaaa aatttgttgt tatttttagt cgtataaaaa agaaacttaa gagtttctta
    10801 agaattgttt aaagaagttg caataaatta gtggtatgat tatagtatga aagagaatgt
    10861 cttgaaattt ttctgtatca aattccaatt atggaggtgt agtatatgaa aaagaaaatt
    10921 tgtgtagcaa taatttttgc tctatttata gtttttgtag aaatgtttat tgatagctct
    10981 agcgaaggaa gtaaaattat gacagctgaa ttaagtaaat acaggaaagg cgtaaatgta
    11041 ttatcaataa acaatattat agtagcgaat aaaaaatata gtttgcccaa agattattct
    11101 cctcaggaaa gcagtgaagc gagagatgct ttttataaaa tgaataaaga tgctcaaaag
    11161 tcaggattaa atttaaaagc ttttagtaca tatagaagtt atgaatatca agatagatta
    11221 tttaagtcat atgtaaaaga acatggagaa aaagaagcaa atagattttc agctaaacct
    11281 ggagagagtg agcatcaaac tggacttgct tttgatattg gaggagatga ccagtcttgt
    11341 tgggcaaata aaaagttcaa caatacaaaa gaagctaagt ggttgtatga aaatgcatat
    11401 aaatatggtt ttatacttag atatccagaa ggtaaagaga atataacagg atatatgtat
    11461 gaatcttggc actatagata tgttggaact gaacatagca aaaactttgc aatgaataat
    11521 ttgactttag aagaatattt acatataaat taagagatgg tttgttaaaa ttaggttaaa
    11581 ataaaaaata acctagacta ttagctgtgt aaagtgatat aatgtaaata caatttaaga
    11641 taactactcg aaggggagta gcttacacga aagtggtaat taatcgtcaa gacagtagaa
    11701 atactcggtt aattagcaag ggattccttg atagcaagac cttcaaaata agcattaggt
    11761 atgtttgttt tggggtcttt ttttattcaa ggaattttta tttaaaaaaa tttaaatttc
    11821 aagtatacat aagagccccc aaacataaaa taaatttatt gaagaggagg gcaacaaatg
    11881 tcttacaaaa gtacttatca agaagtaatt aaaaatttag actcaagttt atctggtttg
    11941 agtaaagaaa gagcagaaga gttgttagaa aaaaatggag ctaatgaatt aaaagaagca
    12001 gataaggttc ctacttataa gcttttccta gaaagtttta aagacccatt agtaataatt
    12061 ctattgatag ctgctttagt acaaatattt ttaggtgaaa cagtggagtc tataataatt
    12121 tttgcagtta ttattataaa ctctgttctg ggtgttgtac aaacaaaaaa agcagaaagt
    12181 tcattagaaa gtttgaaaaa tttatctgca ccaaacgcaa aggtaataag agacaataaa
    12241 aaaatgacaa ttccagcaaa aaatctagta gttggagata ttgtattctt agaagctggc
    12301 gattacatac cagcagatgg aaggcttata gaagcacaat ctctcaaggt tgtagaaggg
    12361 atgttaactg gagaatcaga accagtttta aagcatatag aaaaaataag tgaagatgta
    12421 agtttaggtg accagaaaaa tatggtattt agtggtgctg tagttgttta tggtagaggt
    12481 tcttttgtag tgactggaac aggtatgaat actgagatgg gtaaaatagc tgggctttta
    12541 gaaagtgctg aaaataaact tacaccattg caacaaaaaa tagaggattt tagtaaaaaa
    12601 cttggtgtag gtattgtaat acttgctctt ttaatattta taattcaagt agcaagaaat
    12661 tactttatgg ctggaaatgg agaaatgaca caaattattt tagattcatt catgttctct
    12721 gtagcagtag cagtagcagc tataccagaa gctctatcat caatagtcac tatagtattg
    12781 gctgttggaa caaatagtat ggcaaaaaaa caagccataa taagaaaatt accagcagta
    12841 gaaactttag gttcaacaag tgtaatatgt actgataaaa caggtactct tacacaaaat
    12901 aaaatgacag tagttgatta ttatatgtat ggaactaata atgatgagtt agataaagaa
    12961 aaagtaaact attcttatca tgctaaatta ttgactatgg tttctacatt gtgtaatgac
    13021 tctcatataa ctagtgatgg taaagaagtt ggtgacccaa cagaagtggc tttaataaat
    13081 tattcaagta aaaatgattt agattatgat aaattaagaa gtaagtatat cagaataaat
    13141 gaaataccat ttgactcaga cagaaagctt atgtctactg taaatagtat atatggagat
    13201 tatataatgt ttacaaaagg tgctccagat attgtgttta gtagatgtaa atatgctttg
    13261 aaaaatggtt caaaagtaga tataacagat gagataatag aagaatataa acataaaaat
    13321 gaagagttct caaatagagc acttagagta ttagcttttg caataaaaga tgtcccagaa
    13381 gaagatttta taccttcaat tgatgacgaa catgacatga cattggttgg aataatggca
    13441 atgatagacc caccaagaga agaagtaatg gatgcagtaa aagaggcaaa gagtgctggt
    13501 ataaaaactg ttatgataac tggtgaccat aaaacaacag cagcagcaat tgcaaaagag
    13561 attggtataa tggaagaagg agacttggct cttactggaa aagagcttga tgctttgagt
    13621 gaagaacaac tatatgaaaa actagaaaat ataagtgtat atgccagagt ctcgccggaa
    13681 aataaaattc gtatagtaaa agcatggcaa cataagaata atataacagc aatgactggt
    13741 gatggtgtta atgatgcacc tgcattaaaa caagcagata taggaatcgg tatgggaagt
    13801 ggtactgatg tagcaaaaga tgcttctgct atggtattgg ttgatgataa ctttgcaagt
    13861 atagtaaatg ctgttgaagt tggtagaaca gtatacagta atataataaa atcaattact
    13921 tatctatttg caggaaactt aggagctata gtatccatag tatttgcagt gtttgcaggt
    13981 tggtcaaatc catttacagc tttacaactt ttgtttataa acttagtaaa tgactcttta
    14041 ccagcaattg cattagggtt tgaaaagtca gaaaaaaata taatgaataa acctcctaga
    14101 cacccggatg aaagtatact agcaggagga acattgaagg cagttgctat aagaggttgt
    14161 ataataggtg tagttacaat aattgcacaa tatataggat tacaagtatc tccagaactt
    14221 ggagtagcaa tggcattttc aacattgata ttagctagaa taatgcaaac attacctgca
    14281 cgttctaata atcaaacagt aataaaatta ggttttttca gcaataaata cgttatagga
    14341 gcagttttag tctgtcttgg gctttactct ttaacactaa taccagtgct aagaggaata
    14401 ttctctatac caacaacgtt tggattgtac cagttgggga tttgcctagg gttagcatta
    14461 atttcatcaa tattaatgga acttaacaaa ttaataaata gaagtgaagt aaaataatca
    14521 aaaaattaaa gggcatattg attttaacaa tatgcctttt ataaatagtc taatttattc
    14581 tatattatcg attatttgga atgcttttat aacttcaggt aattcttttt cttcaggcat
    14641 tctaaatctt ataaaatttt tatcaagtcc agtaaaatca cttccagata taactcttat
    14701 ttttaattta gcaaactctt gttgtaaatc tatgtccttg tttttatgtt caactgtcat
    14761 aattgataca gtatcactag tttcagctat attaaggttt ttccatggaa ttaataattg
    14821 attttttata tcttttgttt tttcttttaa ttcttctata aattgaacat catctaataa
    14881 atttgctgca atactacgag atatttcaga cacttcgtaa ggagtagaaa tctttttaat
    14941 agggctaaca agttgttctg gaagaactgc atatcctgct cttagtcctg ctaaaccaaa
    15001 tccttttgaa aatgttttta aagcaattac attatcataa ttatttagaa gcttgacagc
    15061 tgaattttct tttggcatgt attcaccata agcttcatct accataactg ctatatcata
    15121 tttagctgct tcccttacta tattttcaat tgatgataaa ggtataattt gtcctgtagg
    15181 attgtttgga ttgtctatgt aaattacttt atattctgga tttatttttt caataaattc
    15241 tttttcatta aacttgaaat tatcctcttt ctttaaaaga acataatcat aagttgcacc
    15301 atgcattttt atatctgtct catattctga gaactgaggt gaatatccta aaactttatc
    15361 tcccttttca ataaatagtc tatttaaaag gtaaataaca tgtatagaac catctgccaa
    15421 acaaattctg tcagtatcta atgctataaa atctttccaa taatcgacta tattatcttt
    15481 taaagcaatt gaatgaggat atcctcttat catatcaaat tttaaagcat taaaagcttc
    15541 tacagctttg tttgagtaag acacggtatt tataccttca ccacagtcta tctcgaatac
    15601 atcttctgaa tgtccagatt ctattgcata actttttttc aactgcattt caatagcttt
    15661 gttaattttt agtttcatac tttatcactc cttatttagt caataataaa tgttataaaa
    15721 gttgctagat ttaaaatatt atgaaaatat ataatagtaa aaatgaatat aaatattata
    15781 atagctcact ttatttaata tgattatata tcatatattg taaaatttct agcattataa
    15841 gactataaat tgaaaatatt cagaaaaata ctacaagaaa cgatttattt tgctcttaaa
    15901 ataagttaaa tgaatgttat taattttgta aattgaaata gtttaagtat aaagttatga
    15961 tataatctaa taagggtaat tctttagatt atattaagga aatataggta tacaataaaa
    16021 agttatggag ttggattatg ggatataaat taattgtaac agatatggat ggaactttat
    16081 taggcaataa tcataaagtt acagatgaaa ataaaactgc actacaaaaa gttataaaaa
    16141 gcggtattaa tgtaacttta gcaacaggaa gagccttcga ttcagctaaa tgcaatgtag
    16201 attttcttaa agaggacatg ccaataatag cttgtaatgg ttcattaata agagagcaaa
    16261 atggaaatat aatatattca aataagattg atacaagaac atgtttaaac attttagatg
    16321 tattagataa atacgatatt tactaccaat gtaatagtat agattctatg ctttcgaaaa
    16381 aaatagaagg acgtgaagat agattaagtg tatttttagg ctctgaaaca gaagtaattg
    16441 taaaagatga tttgagagaa gaaatcttta aaaaagatat actaaaattt gtaatcatag
    16501 aagaaaaaaa tccatctatt ttggatgaaa taagaaaaga attgagaaaa gttcaaggaa
    16561 taaagataac ctcttcatgg ccaaacaata ttgaagtcat gaatgaaggt gttgataaag
    16621 gtaatgcagt taaaatttta gctgaaaaaa tgaatataga cagagaagat ataattgcat
    16681 ttggagacaa ttacaatgac atagaaatga taaagtttgc tggtcttggg gtagctatgg
    16741 gaaatgcaga agaattgatt aaacaagaag ctgattatgt tacagataca aatcaagaca
    16801 gtggagttgc aaaggcaatt tataaattcc tagagttaaa agaatcactt agctcttaaa
    16861 acagtatata ataggaaaaa ataggttgac tattttgtag atagtaatta taatacaaat
    16921 agataaaata taaaaattta gattcagtga aaaagagagt agatatagag aaaatctcag
    16981 cgaattgggg gatggtgtga gcctaatgat aacactttat attgaaaaga actttggaga
    17041 atactacttg aaattcagta tggtagtagg taatctcgcc ttaagagaat agagtgggta
    17101 aacattgttt accaactaga gtggtaacac ggaatatttc gtctcttggt ctttttgacc
    17161 aagagatttt ttagtttcat tgagttacaa gacttataat taaaatacaa aaaattaaaa
    17221 tatgaggtga aagttatgaa attatggggt ggacgcttta gaaaagcgga aaatcaactt
    17281 atggaagaat ttaataaatc ttttggctat gattgtgtac tgtacaaaaa agatatcgaa
    17341 ggaagtgtag cacatgttca tatgcaagta aaatgtggac ttttaacaga agaagaagga
    17401 aagtctataa cagaaggttt aaaagggata cttgaagatg tagaaaatgg aaagttagta
    17461 ttggatggag agtatgaaga tatacatagt tttactgaga taaatttaat tcaaagaatt
    17521 ggagatgttg gcaagaagct tcatacagca cgtagtagaa atgaccaagt tgcagttgat
    17581 atgagattgt atgctaaaga aaaagcaaat gatttggtgg atttaatatc tgaatttaaa
    17641 gctactataa aagatgttgc tgacaaaaac ccagtgatga tgccaggcta tactcatctt
    17701 caaagggcac aagtagtgac atttaagcat cacttaatgg catattatag tatgtttgat
    17761 agagatgaga aaagaataaa aaatgctata gaaatattag atgaatcacc tttaggctgt
    17821 ggagctttag caggaactac gcatgatata gatagaagta taacttgtga acaattagga
    17881 tttaaaaaag tagtagataa ttttatggat ggagtaagtg atagagatta tttactagag
    17941 ctaatgagtg atttttcaat tataatgatg cacttaagta gattaagtga agagttgata
    18001 ctttggagtt ctcaagaatt tggatttgtt gagatagatg atttatatac aactggaagt
    18061 agtataatgc ctcagaagaa aaatccagat ggtgcagagc ttataagagg taagactggt
    18121 agagtatatg gaaacttatt tggtcttttt acagtaatga aaggaatacc attagcatat
    18181 aataaagaca tgcaagaaga taaagaaggt tttttcgata gtgtacatac tcttgaaatg
    18241 tgtatacaaa taatggatag aatgatagcc acactaaagg taaatgagga taaaatgaaa
    18301 caagcagtaa aaaatggttt tttaaatgca acagaagttg cggattattt ggttaaaaat
    18361 aatgttgctt ttagagatgc acatggtata gtaggaagta tcgttatata ttgtgaagat
    18421 aataaaaaag caatagaaga tttaacatta gaagaattac aaaagttttc agatgctttt
    18481 aaggaagata tttatgattt tatcgactat gagagtatat taaataaggg aataaaaaag
    18541 aatttaaaat aatgtattta tttttaaaag ctgtaaattt gaatgttatc atttaaattt
    18601 acagcttttt attgtgtaag attttattat ataaaagtaa gaaacttaaa aggagtagta
    18661 tttaaaataa atacaaataa aaaattatta ttaaaatgat aattaatatc aataagtatt
    18721 gacaaaatta tttctaaata ctattatata taatataaat aataatgaaa atcaatatca
    18781 aataaatggg aggaaatcta atgaacttat tagtgattgg tggtcatgaa agaatggaaa
    18841 aagattatat ggttatggcc aagaaaaaag gttataagac aaaggtatat actaccatgt
    18901 catctaaatt aaataattct ataggaaaac cagatgcagt tgtcatactt acatcaactg
    18961 tttcacataa gatgtctaga acggttgagt ctcaagctaa aaaacaagat atattaatcg
    19021 taaggcataa aaatagtagt aaggtagcat ttaatgaatg tctagatatg gttgatgaat
    19081 gtctgggaaa ttgttcaaaa tgtaagctta aagacaaaca aaaacaaaaa aatatatagg
    19141 tataaaaaat acataaacat aaaattaaaa gtactgagat aatagttata attattatct
    19201 cagtactttt agatttataa ggataagtat agtataaatt ttttattata atttccaact
    19261 acttgttact ttttcatatt ttttttactt ctagcttttt ttcttttttt cttctttata
    19321 tttgaagcta ttataaataa aactaaaagc actagaatac ctgcaataat cttaacaaaa
    19381 gtcattaaaa cattattttc agtcaaaaat ggaagactgc tatcaagatt attttttgca
    19441 attaagtcta tactgttttc tagtttacca ttattatata catttaaagt accaacctta
    19501 gctccttttt ttataggcaa atcaatttta tttaactctg ttttggtatc ataattgcct
    19561 tgagattcat ctttttctaa gaatatccta taactatttt ttggttcata aatcaattca
    19621 tgttgttttg taaacaatac ttttttagta tctgtgtatt tttctttatc aacaatagta
    19681 gcacacttaa agttatcaaa tccataatca atcagaatac gtgagtctaa atataaatca
    19741 gcattagttg aattaaatac agcaacgata actcttctgc catcttttac agcactcgat
    19801 aaaagacatt taccagcagc atcagtatat ccagttttta taccatcaac tatatcatat
    19861 tttatattta taggttgacc tttgtatgta atcttatcat gtgatgtcaa aaacttatta
    19921 gtgtttacaa agtatctctc ataaggatat gcttttgtag cttcaaattt aagtgactta
    19981 gtttttacaa tttgtctaaa agtatcatta ttcatagctt ctctagctat gagagccata
    20041 tcatgagctg ttgtgtagtg atttgggtct ggtaatccat gaggattatt aaaatgagta
    20101 ttagtagctc caattttttt tgcctcagag ttcatgaggt caataaattt ttgtacattg
    20161 ccaccaccaa catatctagc taatacaaaa gcagcatcat tagcagaatg aaccaaaaga
    20221 gcatctagta actgctttac agtaaaagat tccccatttt ttagatacat actagagcca
    20281 tctatttgag gtaaatcttt aatttctatt acttgattta aatctttaac ctcctttaga
    20341 actaaacaag ctgtccatac tttagttgtt gaagctggat aaagcttaga atttccatcc
    20401 ttattatata gtattttgcc agactcatag tccattaaaa ctgcatgttt agcaactata
    20461 gctggttcat tatctgcaaa agagatatta gagcttccta aaaatgaagt aaatatcaat
    20521 aaacagatta ataaaagtga taaatttctt ttcattgtga gcctccaaat tttaaattca
    20581 tcttttagta tagcatatat ttgtcaataa ttaaaagaaa ggagtgatta atatatgaaa
    20641 aaaaaggtaa aatttatttc gttttttgtt ttatttattt taataatatc atcaataatc
    20701 gtttataaat cgcttatttt caaagatgat atttatgttg taagtaaaaa aaatttacct
    20761 aatgaaaaaa cacatgttgt agagaatgaa attaaaggtg aagataataa tcacagtaaa
    20821 gtaactaaag acatagataa aaattttcaa aataataaaa taactatata cataagtgga
    20881 gcagtaaata gacctggaat tgtaactata gagtcagaca agagacttta tgatgctgta
    20941 gaattactag gaggaacgac aaaagaagca gatttaaatg gagtcaatct atcagtaaga
    21001 ttagaagatg aacaacatta cataatacct aaaataggtg aagcaacatc tgttacaagt
    21061 aatgatgatt ccaataaact aaatcagaag aatgagtcaa gaggtgagcc taaaaataaa
    21121 tccaataaaa atatatctaa cgaatcaaaa gtaaatataa atgtagcgac gatagaagag
    21181 ttggattctc tacctggggt tggagaagct acagccaata aaattttaca acacagagaa
    21241 gaaaatggac agtttagttc aattgaggaa ataaaaaatg taaatggtat tggagataaa
    21301 aagtatgaaa atataaaaga tttaatttgt gtagactaaa atataataca aaacttacat
    21361 atagatatgt tattataaat atgtatgtat atgtgtgtac atgtggaaat ttagtaaagg
    21421 aggcactttt atgaaaaaag aacaaaaaaa acttacagag atgacaacag caggtggttg
    21481 agccgctaaa atagggccag aggttctggc atcagtttta tctcaattgc ctaaaaatga
    21541 taatatagaa aatttattag taggactaga tacagcagat gatgctgcag tttataaatt
    21601 aaatgatgat atggcactta tccaaacttt agacttcttt acaccaatgg tagatgaccc
    21661 atacgtattt ggtcaaattg ctgcatcaaa ttcactatca gatgtatatg ctatgggagg
    21721 caaacctttg gttgctatga atattgtttg ttttccatca tgccatgata tggatgtact
    21781 tgcagaaata ttaaaaggtg ggtttgataa agtgaaagaa agtggagcac ttttggttgg
    21841 agggcatact gtagatgaca aagagcctaa atatggtctt tcagtttctg ggattgtaag
    21901 tccaaataaa gttttatcaa atgctactgc aaaacctggt gataagttaa ttataacaaa
    21961 accaattgga gtgggtgtat taaatacagc catgaaagaa ggtatggtag aacaacatat
    22021 agcagataaa gtaatagaaa taatgataca tttaaataaa tatgcagcaa tgagttttga
    22081 gaaatttgat gtaaattcgg ttacagatat aactggtttt ggtctacttg gtcatacttt
    22141 agaaatggcc aaagcttctg aagtaagtat tgaaattgaa tcaaaacatg taccaattat
    22201 tgaaggtgca atagaaatgg cacaaatggg aattatacct gctggtatgt ataaaaatat
    22261 gcattatgta tctaaagatg tagaagttgt aggaaatata gaagttgcag ttcaagatat
    22321 tttatacgac ccacaaacat caggagggtt gttaatttca gtaaaagaag aacttgcaga
    22381 ggagcttgta aaagatatga aattaaatgg agcaatagaa gctaaaatta taggttctgt
    22441 agttccaaaa ggggaaaaat atattacagt tttataaaat attatagaga aagagtgatt
    22501 gttttgagca aaagagaatt gtttgcaatg ttgccttcag ttgatgaagt attaagtgat
    22561 aatagaattg tagaaataat aaatgaatat cctagaagtt tagttttaga atctgtgaga
    22621 gaagtaatag atttaaaaag acaatttata ttaagattaa aagaagatgc atcaaactct
    22681 gtaacaatag agtttggtga aataatagaa tctgcaatag agagagttaa gttaaattac
    22741 tcattatctt taaaaaaagt tataaatgca actggtacag ttattcatac aaatttagga
    22801 aggtctttac ttagtgaaga tataaaagat gaattatggt gtgctgcttc aaggtattca
    22861 aacttagaat acgatttgga taatggagaa agaggttcaa gatactctca tttaactagt
    22921 acaataaaaa gacttactgg agcagaagat gtactagttg taaataataa tgcagcggca
    22981 gtacttcttg tacttagcac aatggctaaa ggaggagaag caattgtatc tcgtggagaa
    23041 ttagttgaag taggtggttc atttaggatt ccaagtataa tggctcttag tggtgcagaa
    23101 ttagttgaag ttggttcaac taataaaact catttgaaag attacaagga ggccattaca
    23161 gaagatacaa atgtattgat gaaggttcat acaagtaatt atagaataat gggattcaca
    23221 gagagtgttt ctatagaaga attggttaat ttaggtaaaa aatataaact acctgtaata
    23281 gaagacttag gaagtggagt ttttatagat ttatcaaaat atggtttatc ttatgaacca
    23341 actgtattgg attctattcg acaaggtgca gatgtagtaa cttttagtgg agataagatg
    23401 cttggaggtc cgcaagcagg tattatagtt gggaaaaaag agtatataga aaaaatgaag
    23461 aaaaatcaac ttactagagc acttagagtt gataagctta caatatgtgc tcttgaagca
    23521 acattgagaa tgtatttaga tgagacaaaa gctatagaaa atatacctac tttaaaaatg
    23581 ttgacatata aaattgaaga attagaagta aaagcaaata aactatttga aaaaataact
    23641 gctttaaact taaatgcaaa cataaatata gaagatggat tttcacaggt gggaggaggg
    23701 tctatgcctc ttgaaactat aagtacaaag gtaatatcaa taactcctga acatatgaat
    23761 gtttcatctt tagaaaaaaa actaagactt agtgaagcac atataatagc cagagtgtat
    23821 gataataaat atgtactaga tgtaagaact atatttgatg atgaatttga tgtaatagtt
    23881 gaggaattga gaaaagcatt taattaaata aatttgagtt aataaatatt aaatttagaa
    23941 gtataagtat gaagttttga ttaaaaaata gctacacaca cttgtgactt tagtcatagc
    24001 aggtttagag ggggcaaaaa atgaaaaatg tagtaattgg aacagcaggt catattgacc
    24061 acggaaaaac tactcttata aaagcattga ctggaagaga gactgatacg ttagatgaag
    24121 aaaaaaagag aggtatttca ataaacttag gatttactta ttttgattta ccaaacaata
    24181 gaagagctgg aattgttgat gtcccaggac acgaaaaatt tataaaaaac atgttagctg
    24241 gagcatcagg aattgatata gttttacttg ttgtagcagc agatgaaggg gttatgccac
    24301 agacagttga gcatttagat attctaagct ttttaaatat caaaaatgga atagtggttc
    24361 taactaagtc tgatactgta gatgatgaat ttagagagct agtaaaagaa gatatttgtg
    24421 aaaaaataaa aggaactttt cttgaagatg ctgaaataat tgaagttgat tctatatcta
    24481 aaagaggtat agacactctt attgaaaaaa tagataaaat gagtgatgat atagaagata
    24541 aaaatgaaaa ctctccagca agacttaata tagacagagt attttcaata aaagggtttg
    24601 gtactgtaat aacaggcact ttaatagaag gaaagataag tatagaagat gatttggtta
    24661 tatatccaca ggtattaaaa acaaaaataa gaagtataca agttcatgga gaaaataaag
    24721 atacagctta tgcaggtcaa agaacagcaa tcaatatatc aaatgtaaaa gtggatgata
    24781 taaaaagagg agatgtttta gctgcaccaa actcattgga ggaatcaatg atgttagatg
    24841 taaaattatc attggtaaat catttagata aaggattaaa gcactgggat agattaagat
    24901 tgtatcatgg aactagagag atactttgta gagcagttcc actagataaa gaattaattg
    24961 aaagtggaga aagtggctat gttcagttga gacttgaaga aagcatagta tctaagaaag
    25021 gtgatacgtt tgtagtgaga cgttattctc caatggaaac tataggtgga ggtgtaataa
    25081 ttgacccatc tcctaaaaaa cataagaaat ttgatgaaaa agttatagaa gcattaaaaa
    25141 taaaagaaaa aggcgaacta aaagatataa tagaagagta cttaaaaagg aatttaaaaa
    25201 attatccaaa tataaaagaa ataatgagct atagtggtgc tcatgaagaa gatgtaaaaa
    25261 gagcgcttga aacacttata tcagaagata aagtatttat tataggaaat atgtatatgc
    25321 atataaatca atacaataaa ttaaaagaaa atacaataaa gcttttaagt gagtaccata
    25381 agaagtatag gcttagaaaa gggatactaa aagaagaagt aaggtctaag atagagagta
    25441 attttaagac tagggagatg gatattttat tagaaaaatt gtctacagaa aatgctataa
    25501 aaatagaaaa taatatagta tctctattag attttgaggt tattttaaat gataagcaaa
    25561 aagaaatagc aaaaaaaata gaaaaaagat taaaatcatg tggagtttct tctatactga
    25621 caatagatga agtttctgaa ggaaatcata actatgctga agtattggag tcaatgatag
    25681 gtaataaagt agagaaatta gatgatttgt atataatgga caaagatatt tatgaaaatg
    25741 caaagaacat tttgataaat tatataaaag aaaataaaga aataacatta ggagaataca
    25801 gagatttgat tgattctagc agaaagaatt gcatgattat attggaaaac ttcgatagaa
    25861 acaaaataac taaaagagta gaaaacaaaa gaattttgtt ttagcagaaa atgtctgttt
    25921 ttgctttaag gtataaagaa ggaattataa tgtcgagtat cgtataatta attagtataa
    25981 aatattaatt aagaggaagt gtaaagtatt gaagaatata atacctgatg taaaaccaga
    26041 tataaaagaa agagaagaca tgagcctttt atttataata atgggctcag tacttttatt
    26101 atttattata aaagatatag agaaatatga aactttatta catgccatat atacgatatt
    26161 tttagtagct acaggagtaa ttatatttaa tactacaaaa tttaaagtag attcattttc
    26221 tacattttta ggattaattt ttatagctac tggagtattg gagtgtatat atttatttaa
    26281 ttccttgggg attaaaacta agtctataat ggagattaac atcacaatat cagcaataac
    26341 agatttattt cctatactgg gtgtatactt aagttttaaa tttgttaagg ataacaaaca
    26401 aatttattca agtgtaattt tatttatagc aactgctaca ttaacaataa catctttatt
    26461 cataatatca ggaatttgtg actatctggg aaatggtaaa tcacaatatg ctttaggagt
    26521 tataatatct atattcataa taattatttc tatcattagt ggaattgaat taaattcaag
    26581 ttcaaataaa ggtaaatggg aatatgatga aaaaaagatt tttaacagaa taatagtaat
    26641 aataatctta tcaagagttc caaatctatt gcatatagtt attgataata gaaatgtaga
    26701 gaggatatta tcacaaataa taataaatgt agccttatat tatttatata attacatagt
    26761 atctaagaat attaagaaaa cagcactgga attgcatgat acgaatgaag aattaacaaa
    26821 aaaaacggaa agtttaaaag aaaagaataa aaaattaatc tatgagacag aaaagataga
    26881 ggaacttaag ggaatactta tgaaaagaga atctaggcta caatctacat tagatgtagc
    26941 agtaaatagc atagtagtat ttagcaaaga tggagaaatt acatatgcaa ataagagctt
    27001 tagaaatact tttggagact ataaagaaca agataaattg actgataaaa taaaaaactt
    27061 caatgatttg atagaaagca ttcattatgt gtttgttcat gagaagaatg cagagaaact
    27121 tattagtaca agcgataata aagtttatca agctatattt gcacctttaa ttattgcttc
    27181 acaaacagaa ggagtattgt gtgtactaga agataagact aagaaaaagg agtacgaacg
    27241 caaacttatt gaagcaaata agagaagtaa agatttttta gaaagtattg gagatggcat
    27301 agtagtgctt gaaggtaata acaaaatata tgttaataat gcatgtaggg aaatttttaa
    27361 aaataattta gagagtattg atttttcatt agtatgtaaa agtgaagaaa gtatagaaaa
    27421 aagatatgta atagatggtg aagtaaagta tgtagagatg agtttttctc agtatactaa
    27481 tgctggtact aataaaacta ttattgtaat acgtgataca acctctcgaa aaatatctca
    27541 aataaaactt gaggaaagtc aatcatcata ttcaagattt atagatatat tacctgatgg
    27601 catatgctta ttaaaagaag atttaagtat aaactatgca aataagtcgt ttttagatat
    27661 gctagctttt acaaacatag atgatataaa agattctaat attaaactat taatgaatgc
    27721 gaatactgaa gaaaaaatga aatttacgga taatatggct aaagttttaa atgaaaatag
    27781 aagtatactt ttactggaac atgaattgat aaatagttat gatgatatag tagaagtaga
    27841 attaagtgca ttaccttttg ctatatacaa tactagatac ataatgctta ttataaaaga
    27901 tttagtacat aagaaatatt ccgagcaagc agaaaaagaa ctgcttgaaa gatttaaaac
    27961 agataaaatc aaaacggagt tttttgcaaa tatgtctcat gaactaaaaa ctccactaaa
    28021 tgttatatct agttcaaatc aactggtaga ttctttctat agaaatgaaa agataaaaga
    28081 ttacaataac aatattaaat ctcatgtgga tttagttaga caaagttctt ataggcttca
    28141 aagattaata aataacatta tagatttaac aaagatggag tctgggttct atacactaaa
    28201 acttgcgaaa tacaatattg tatctgtaat agaagactta tttatgaata tagaagagta
    28261 tgcacttaga aaagacataa aaattctttt tgatacagac cttgaagaaa ttaatgttta
    28321 tatagacaag gttgaaattg agagaattat gttgaatcta ttgtcaaact gtattaagtt
    28381 tactgataat ggaggctgga tatatgtaag tattcattat aaaatagata aagtaatcat
    28441 aagtgtaaaa gacactggag ttggaatacc acaagataaa ttagagttga tttttgaaga
    28501 attttcacaa gttgataaga ctttatccag aaatactgaa ggaagtggaa tagggcttgc
    28561 tatagttaag aatctagtaa gtcttcatgg tggagatata gaagtagtaa gtgaagtgaa
    28621 taaaggcaca gagttcttaa tttcacttcc aatgaaggga tttagtagtg aacattacac
    28681 agaagataag agaatatata atattcaaga aaaaataaaa atagaatttt ctgatatata
    28741 ttattaaaaa tgattatgag caaaaataaa ataaaaatac tatttgttta aatatttaag
    28801 ttgtaagaaa taggctatca taaggtaaat tattatgata gcctattttt agctaggttt
    28861 tatatctata tgtgagaaca tataatctct aaattacctt caaaactaaa atctccaaag
    28921 tcgtttggca gtataaaatg catacccttt tttaaattat attctacaga attgtgtata
    28981 agttttccat ttccatccaa cacactgtat aaagaaaaag gtgtattgtg tgtaaaatta
    29041 cattttccaa aaacatctaa tttataaaca ctaaaaaatt cgtttgacac aaatgttgtg
    29101 catttaaagt ctgatgttga tacgatttta tagtcagtat cgaaatttat atgaggaaca
    29161 ttcgtaacat ctattgattt ttgaacatga agttcacgct tattacctga attgtcagtt
    29221 ctatcataat catagactct ataagtagta tctgagtttt gttgagtttc taaaatcaat
    29281 gttcctttgc atatagcatg tatagtgcca cttggaacat agaaaaagtc tccctttttt
    29341 atattaacct ttctaagtag tttgtcccat tcattattat ttaccatatt tacaaattct
    29401 ttatgagatt ttgcattatg acctattata atttctgcat catcagaaca atctattaca
    29461 taccaacact ctgtcttacc taactctcca ttttcatttt ttttagcata ttcatcattt
    29521 ggatgaactt gaactgataa attatcattt gcatccaata tttttgtaag taaaggaaac
    29581 ttatcaccag gtgtgtttcc aaataattct ctatttttat tccacaattc agatagtttt
    29641 ttacctttat attttccatt ttctattaag caatctccat ttttatgaga gcttatagcc
    29701 caacattcac cagttgttgg cgaatctatt tcataattaa atttatcttt aagtgcagta
    29761 ccaccccata ttctatccat aaaaataggt tttaaaaata atggttccat aatcctgttc
    29821 ctcctatgat attaaattta tcctacaaat aattatatat aaatagatta taaaatcaat
    29881 agtaaaaaag ttttgtccct tgaaatgaca taaagtataa ggagttatta aataagataa
    29941 actatgatat ataatataaa gttctaaatt ggagtgataa ttatggagtt gtttaaggca
    30001 gaaagcaaaa aatataaaaa agaattttta aaattattgg agcaatgggt tagtatacct
    30061 tcattttatg atagaaaaac tgtatctaaa gacatgcctt ttggaaaagg agtatatgat
    30121 gcattaaact ggtttgaaaa tctaggtaga gaaaacaatt tcaaagtaaa aaatatagat
    30181 aatcatgctg ttcaaattga atatggaaat ggtaaagagt atgtagatat atttggacat
    30241 tgtgatgttg taaatccagg tgaaggatgg gatagtgaac catttaaact aaatattata
    30301 ggtgataaat tagttgctag aggtgtatct gataataaag ggcctatgat agtgaatttt
    30361 ttagcactaa aaatgataaa ggatttagat attaatttaa aaagaaaggt tagattaata
    30421 gctgggggaa atgaagagag tggatttaaa tgtataaagc attattatag caaagagcca
    30481 tatggtgtat gtggatttac tccagatgct aaatttcctg tattaaatgg agaaaaaggt
    30541 ggagcaataa taaagttaat ttcaaatata gacgataaat ctttatatat aagtggtgga
    30601 atagagttta atacaatacc agataaagtt tatataaaga atgtagaaaa attgggtaaa
    30661 gataatatat gttttgatat taacaatata tcgataaatt atgataatgg caattatatt
    30721 gtgtatggaa aaggtggtca ttcatctaaa cccgaaaaat caataaatcc tatattagct
    30781 acaattaaac ttttgtcaga aaatattgat gaaaaatgga caaaagattt atataagttg
    30841 ataaatcaag ataatataaa tggaaactta tttggtttaa atattgaggg gaaatgtgga
    30901 attttatcaa tggtgccaac aataataaat atagtagatg gaaaattaga agtagtttta
    30961 agcgttagat atccagaaat attaaccatt gaagatataa taaaaaaatt taatttgtat
    31021 atggagcaaa ataatattaa taaatttgag cttattgggg aaaatttaaa gcaagcaaat
    31081 tatatagata gaaattcaaa acttgtaaga agtttgcatg atatatatat aaagtattct
    31141 ggagatttaa aaaatgatgt tagagtaact agtgcaggaa gttatgcttc tgaaatgaat
    31201 aattcagtaa tatttggttg tgaatttcct gatggtagct ttggcaatgt ccatagtgcg
    31261 aatgaatttg ctagcttaga tagatttata actgcaatag gtatatatgc agaggcaata
    31321 attacattat gtaataaaat ataaatacaa aatgtccctt atatagacat attatatata
    31381 caattaaaat tttacttgat atataattga aatcaaagag aaggtgatac aatgataact
    31441 aagaaacatg caataatagt aaagagttta agcaataaag atggatatat gacatcaaac
    31501 gaattagcta tcaaattgga tgtgtctact aaaactatta aaagatatat agcagattta
    31561 aatagtgttt taagcaaata tgatttggta atagcttctt ctagaggtat aggatacaag
    31621 ttaagcggtt ctaaaaacaa tatagccaga gctgttaaag aagcaaataa atatattgat
    31681 ggttttttag atgactctga agagtcaaga atgtcaaata taatatgtat gcttattaat
    31741 agaaattata tgagtataga agcaatggca gaagaattaa atctaagtat agccgctata
    31801 aataaattgt ctagtaagct taaaagaaaa cttgaaaagt atgacttagt tataaaatct
    31861 aaaccttact atggaagcta tatacatggt gaagaaatta atataaggca attaattact
    31921 gactatgcaa taaaattaga tgaaaaaagt aaagtgaagg tgtttttaga tgacattagt
    31981 gaaaacgata tacactgtat agaaagtatt ttagaaaagc acttaagaga aaaagataca
    32041 ataatttcag ataaagattt taatcttttg ctttcaaaaa taatcgtttc tgtatttagg
    32101 agtaagagag gtcatagtaa caatataaac ttaatggata caagttatag atttcataac
    32161 tactacttta tagaaaatct aatgaaagaa atatctgata aaattggatt taaacttatt
    32221 gaagatgaag taatttatat ttcaaattat tctggtgtaa tagcctataa aggtacacaa
    32281 ggaagaaaaa acacaagtga aattgaggaa cgaataagta gtgttataag tagtgcactt
    32341 caagatatat tccttatttc aggtagtgat tatacaaaag atgatgagtt tatggttgca
    32401 atctctgacc atataaagag atttttaaat agagctagag ctaatgtaaa atcaaataat
    32461 cctttacttc atcaaataaa agagaagttt cctatagctt ttaatttagc cgtgttttta
    32521 tcaaataaac tagaaactga gtttaattta aaacttgatg aagatgagct tggatatata
    32581 gctatccact ttgctgcttc aaatgaaaga atgaaaaaaa atactagtaa gaaaatttgt
    32641 atagtttgtc attatgggat aggaacagga caacttattt ctgaaaagct aaaacagaat
    32701 ataagtgatt taagtgttgt aggtgtttat ccagttagat atctcgatat ggctataagt
    32761 caagatgttg atttgatagt atctaccgtt gaattaaaag gatatgaaaa acctgtctta
    32821 tatatagaaa atatatttga tgatagctta attgaaaacg taaacaaagc cttttatgaa
    32881 aaggaagaaa gaagaaagat aataagcaac atgtttgatg aaaaagcttt tttcagtata
    32941 aaggctaata ctaaagaaga ggtcataatt tcactttcaa ataaactaaa agaaagagat
    33001 tttatagagg aaagttctat aaaaagtata atagatagag aaaatatttc atcaactgaa
    33061 ataggaaatc tagtagcaat accccacaca atagtgaagg gagataaaaa atctataata
    33121 ggagttggaa tacttgaaaa tccaataatt tgggataagc aagaggttca gctagtattt
    33181 atggtatttt ttaatactaa agaaaaacat aatttctcga tatttaaata cctttataat
    33241 tttataaaag atgaaggcgg agtaagagga attattaaaa tatgtgactt taataaatta
    33301 atggcactga taggtaatta aggaggaaat taataatggg aacaaacata tttaacaaag
    33361 aatatgtatt tttaaatgtt gatgctaagt caaaggttga agttttaaaa tttatatcaa
    33421 aaaaagctaa agacttaaac ttggcagaag atgagagctt agtatatgaa ggtttaatgg
    33481 ctagagaaga acaatttact acaaatttag gagaatctat agcaatacct cacactaaaa
    33541 atgatgcaat tgaaaatcca gcagtagtag tattgaagtt taatgaagat gttgtttgga
    33601 atgaaggtga ggataaggtt aaattagcaa taagcttact tatgccagga aaaagtaaag
    33661 agaatattca tcttaaatta ttatcatctc tttcaagaaa attaataaat aaagaattta
    33721 aagattctct tttaaaaagt gataatgttg aagaaatatc taacttaata aatgaagctt
    33781 taggtttata attaaacact aaggttataa aataaaaaaa taattaacta aaaataggag
    33841 gaatttgaaa tgaataaaaa attagtagca ttatgtgctt gtccaatggg actagctcac
    33901 actttcatgg cagcagaagc aatagagcaa gcagcaaagg cattaggtta tgaagcaaaa
    33961 gttgaaactc aaggtgctga tggtgtacaa aatgagttaa caagagatga tatattagga
    34021 gcaactatga taatacatgc agtagcaata acaccagaag gtatggaaag atttgatggt
    34081 tgtgaagttt atgaagttga attacaagaa gctataaaaa atgcagaagg tgttataaaa
    34141 gaaatagaag aagatttagg aatataataa atttattgtg gaggaaatag aatggcaatt
    34201 aagaaaaaag ttataaacag ttcaggtgct tctacaacag gaggaaataa tgtaggaaaa
    34261 tctactccat ctagtccaac tcaatcaaag ggaaatggaa aatggaaaga aataagtaaa
    34321 catataatga caggtatatc atatatgata ccagttttag taatgggtgg acttataggg
    34381 gccttatctc aattaatacc atatgctata ttaggacttg acccatctgt aggtatagtt
    34441 gatgctatga actcaggaga atttacaggg ttcaagctta gtcttttaaa tatagcacaa
    34501 ttaatgtcaa atttcggatt tactttgttt ggatttgcga taccactgtt tgcagcattt
    34561 tgtgcaaact ctataggtgg aaaaacagct ttaattgcag gatttatagg tggatatata
    34621 gctaataaac ctgtaggtgt accacaattc gttgatggac aatggactga agttgttcca
    34681 gttgcatcag gatttttagg tgcaatacta atagcattta taataggata ttttgtaaaa
    34741 tggttaaata aatctataaa agtttctcat aactggttag cttttaaaac aacattttta
    34801 ataccattaa tagcgtcatt agcttgtatg gtattgatga tatttataat aactccattt
    34861 ggtggattaa ttaatgaaag tatgaaaaac ttcttaacag cagcaggagc agctggagaa
    34921 tatgtatatg caacagcttt ggcagcagct acagcattcg acttaggagg accaataaat
    34981 aaagcagcag gattcgttgc acttggttta actacagaga atgtacttcc aataacagca
    35041 agaacaatag ctatagttgt tcctccaatt ggattagggt taacaacatt attagataaa
    35101 agattagtag gaagaagagt atatgataga caattctatc aagcaggaaa aacttctata
    35161 ttcttatcat ttatgggtat atctgaagga gcaataccat ttgcacttga gagaccaggt
    35221 tttgttatac ctttaaacat agttggaagt gtaataggag ctattacagg tataattcta
    35281 ggagcaattc aatggttccc agagtcagct atatgggcat ggcctctagt agataactta
    35341 tttggatata taataggtat agcagttggt gctatattca tagcagtagg aaatatattc
    35401 tatagaaata aattaataaa agatggtaaa cttgttgtag attatatcga ctagtattta
    35461 atttttacta tctataaata actttggctg gtaatatggt tttaatcaat attaccagcc
    35521 ttacctatat gattaattaa aggcggtgga gataagatat ggataagata gaaaaagtaa
    35581 gagagtattt tagagaatat gatatagatg gatttcttat aaatagtcct acaaacaagt
    35641 tttatgttgg aaatttattt agctcttcag gttatgtttt tataacgaaa gaatcacaat
    35701 atataatagt agattttaga tattttgaag aaattaaaag aaaatctagt ttgtttaatg
    35761 tagtattaat ggacaaaact agaactcatt ttgatataat taatgatatt tgtagagagc
    35821 agaatataaa ggaaattgga tttgagggaa atgaagtatc ttttgattta tatagaagta
    35881 tgagtaataa gttaagtgca accttaaaat cagttgattt atctacatta agggaaacaa
    35941 aaaatgaaga cgaaataaaa tatataaaaa aagcttgtga aatagttgat gctacatttt
    36001 atcatatagt agactttata aaggttggta tgactgaaaa gcaagttgaa aatgaaattg
    36061 ttagaattat aaaagattta ggtggtcaaa aagaatcttt cgatacaata gttgcatctg
    36121 ggcttagagg agcattacct catggaaaag caagtgaaaa agttatagag tatggagatt
    36181 ttgttacctt tgactttggt gctaagtata ataattattg ttcagatatt accagaacca
    36241 tttgtatggg tactataaat aaagagttag aagaaatata taatatagta agaaaagcta
    36301 atgaagagtg tataagggtt ttaagaccgg gaatgactac tggagagata gataaagttg
    36361 caagagacat aattggttca tatggatatg ctggtaattt tggtcataat ctgggtcatg
    36421 gtgtaggaat tatggttcat gaatatcctg cattagctcc tgaatcaaat gaagtattaa
    36481 aagaaggaat gattgtaact atagagcctg gtatatatgt tccaagtctt ggaggcgtaa
    36541 gaatagaaga tgatgtactt ataacacaag atggatgtat gagattgact acatctacta
    36601 aggatttaat agttgtaagc taaaaataaa ttagataaat attttataac aataagaaaa
    36661 gaagaggtaa atttaaatgt ataattttga tgataaacca gatagagtaa gtgaaaagtg
    36721 tagaaaatgg gatttaaata ttataagaga taaatttgga gatattagag aagattttat
    36781 accaatgtgg attgctgata tggattttaa aataccaaca gaaatagaaa acaaatttat
    36841 tgaagctgta agaagaggtg tatttggata tacatactgt tatgatgaat tttatgattc
    36901 agttattggt tggcaaaaaa atatgcatga ggtaaatgta gaaaaagaat ggattacatt
    36961 aacttatgga actgtatcaa cacttcatta tgtagtacaa gctttttgta agaaaggtga
    37021 tagtataata ttaaatacac cagtttatga cccctttgaa tcatcagcaa aaaaacaagg
    37081 tgtaaatgta atttgtaata cattagatgt tgtaaatgat agatattata taaattttga
    37141 taaattagaa gttcaaatta aagaaaataa gcctaaatta atgatgtttt gtacaccaca
    37201 taatccatct ggaaggatat ggacaattga agaaatgaca agggtagcaa ctatatgtaa
    37261 agaaaataat gttattttgg ttgcagatga agtacatgca gagcatattc attatggtac
    37321 atttaattca atacttaaga ttggaaaaga gcttttagaa aatgttattc tattgacatc
    37381 accaaataaa ggatttaatc ttggtggatt aaagacttct tattcaatag ttataaataa
    37441 ggatataagg gacaaattta gaaataaatt aaagcaaaat tctataactt ctccaaatgt
    37501 gtttggaata ataggattga taaccgctta taatgaatgt catgagtggt taagtggtgt
    37561 aaatgaatat ataaaatcaa attatgaact tttagaaact tgggtaaata aatataataa
    37621 gataaaattg atgaaaatgg aatcatctta cttagcttgg atggatataa gtggattagg
    37681 tattagtgca agtgagttta cagataaatt ggctatagat acaggagtat tattagaaga
    37741 tgggagtcat tttgtaaaag atggtgagaa gtatgttaga ataaatttag gaactcaaaa
    37801 ggaaaatgta attgaagctt taaatagaat ggatttattt ttaaagtctt tataaaaatt
    37861 tggttgaatt agtattttat ataaatttaa actattaata tggtatatac ataagtagaa
    37921 tttgttttag attacttaag tatataccat attttatata aaaattaaat ttgcattagt
    37981 catgtgataa ctatttaaat tcatatgtat tttgtatgta taatagatat aattattatt
    38041 taaacttatt gtttatttaa tttttcaata gaaaaagatt tatctgtaag tttaaaatta
    38101 tcttttatac ccttagttaa gtatactttc ttatccttag ttatgaatat agcatctaca
    38161 ttatcaattt tattaattaa attcatacct ttttcaagac caagaccaaa tgtagaagtg
    38221 gataaggcat cacaattaat agatttatca gagattattg taacactact taaattgttt
    38281 tcaaaaggat accctgtaaa tggatttaac atatgatgat atatttttcc gtttttttcg
    38341 ataaatcttt cataaattcc agaagtaaca acagacttat ttgtagtttc tatgtttcct
    38401 atggattcac catttttact agttgggtct tgaataccta ttttaaaagg tttatcattt
    38461 ttaccatttt ctaaagtaaa tatatttcct cctaagttta ctaaaccttt ttttacgtcc
    38521 tctgatttta aataggctac aattttatca gcagcatatc ctttagctat ggcacctaaa
    38581 tcaattttca tattttcttt ggttaatttt atagatttat ttttttcatc caatatgatg
    38641 ttcttataat caataagagg cagaagtttt tgtatctcag aatcacttgg aactttagct
    38701 ttatctgttc caatagccca taaatttgat aatggaccaa ttgttatgtc aaaatatcca
    38761 tctgataatt tggaatattg tatagcttct tttacgacga aaaatgtatc atcagaaact
    38821 ctgacaaatt ttttaccagc attatcattt attttagaaa catcacttcc tggtatatga
    38881 gtgctcattt tgttgtcaat atatcttaaa attgaatcac attcatttaa gattttatca
    38941 cttttggatt cttttacatt aaatacagta acttcgttta ctgtaccaag gtagtagcta
    39001 gttttactat atttagtatt ctggcttgaa ctcctaaaaa atgaaaataa aattaatgct
    39061 aaaatcaata tcacaaaaat aaatgtgatt tttttcttat tcatggtata ttcctcctat
    39121 gaaattgtgt tattatttta ttataacata atttcatagg aggacaaaaa atgaagaaaa
    39181 aagacattct tcttatttta ggcattttgg ttgttattac agcatgttat gggataataa
    39241 atgttataaa ttctaaaaat gctggaaata ttgagattta tgtagataat aaattgtata
    39301 aaactgtttc aataaatgcc aaagaagaat ttaaaataga gaatagaggg gggtataaca
    39361 tagtaaagat tcatgataaa ggggttgaga tagtagatgc ttcatgtcct gataaggttt
    39421 gcgtacatac tggatttata aacaagccaa gtcaaagcat agtttgtata cctaatagag
    39481 tgagcataaa aataaataca aatgaaaaga atgataatca agaagatata atttctaaat
    39541 aaaataataa aaataattga aaggaattgg tgataattat ggttaactta ggaaacgatt
    39601 gggatgaatt attgaaagaa gaatttgaaa aagattacta cttaaactta agaaaatttc
    39661 ttatagaaga atataaaaca cgccaaattt tcccaaatat gcacaatata tatgaagctt
    39721 taaaacacac ttcatacaaa gacacaaaag tcttaatatt aggacaagac ccttatcatg
    39781 gagataatca agctcatggt ttagcttttt cagttcaacc acaagtgaaa actccaccat
    39841 ctttattaaa tatgtataaa gaattaaaag atgatttagg ttgttttata cctaataatg
    39901 gatatttaat gccttgggca gaccaaggag tgttactttt aaatactgct cttactgtta
    39961 gagcgcatga agctaattct cataagaaca aaggatggga aattttcact gatagagtaa
    40021 tatctatttt aagtgagcgt gaagacccag ttatttttgt actatgggga agtaatgcac
    40081 gtaaaaaagt agagttgatt gatacaagta aacattatat tttggaagca cctcatccaa
    40141 gcccattgtc agcaagtaaa ggcttttttg gatgtaaaca cttctctaag ataaatgaaa
    40201 ttttgaaaaa gctaggaaaa gaacctatta attggcagat agaaaatata taaaagtaaa
    40261 aggtaacaaa atgataacaa aacaacaata aacaatgaca ttgttgtaac taatagaaat
    40321 atgcttaaat agttgaaaat actatgtatg catatttcta tttttttgtc aagttgacaa
    40381 ctcttgaaaa aatagttaca atttagttac acaaaaaaca caaataaaaa aagaaactta
    40441 caaagcagaa ttttatgaag caaaatatat agtgtaaaaa atcttaggga ggaaatttta
    40501 tatgaatttt aaaaaattga gtaatgctaa agtctcggta atggcagttg ttttatcagt
    40561 tggaatatta tcgggatact ctgcgcttaa taaacaagta acattagtag taaggggaga
    40621 agaaagaact atatcaacat ttagttctaa tgtaggagac cttttagaat ctcaaaacat
    40681 tagctatgat gcaaatgata tagtaacttt agatgaaaat acaaagttat ctaatggaga
    40741 taaaatagaa gtaattgatg taaaggaaaa aacagttgta gaaactaaag acattccatt
    40801 tgaagttaca gttgttgaag atggctcttt attaaagggt gattctaagg ttaaagaaga
    40861 aggaaagtct ggtaaaagtg aattaatata taaagaaact tatcacaatg gaaagaaagt
    40921 agaaaaggaa tttgtgaaaa aggttgttaa agctgagcca gtaaataagg ttgtaagcaa
    40981 agggacaaag gtagaaatgc aagtagcctc atcaagggga gaaagttcaa gaagaatggc
    41041 ttcatcatca tctagtagca gtagtagtaa caatggtaaa aatatgagag tggtagcaac
    41101 tgcatatact ggtcacagta taactgcaac aggaacaaaa cctaagtggg gaacaatagc
    41161 tgtagaccct aaggttatac catatggaac taaagtttat atacctcaat ttgacatggt
    41221 gtttactgca gaagactgtg gtggagcaat aaaaggaaat aaaattgata tctacatgaa
    41281 tgattcaaaa acagtttata actggggaag aaaaactata gatatacgta tattaaaata
    41341 gttaaataat ttatattttt actggaaatt atattttgat tatatagaga agaaactctt
    41401 tatagattct aaaaatataa tttccagttt tttattttgg atatgtaata aaacaggtaa
    41461 attttaacca tgtatagata aaagctattt aaattataat ttactatcat ataaatctat
    41521 aaaaattgtt gcaaagagtc aaaatatatg atattataga tttcgttgag aaggatttca
    41581 ttatgggagt ggatgggtgc cggtgtgccc tctagtcttc aaaactagta tgaggggtta
    41641 agagcctctt aggtgggttc gattcccacg cattcccgcc aatatgtaaa aggtatcttt
    41701 taattaaaag ataccttttt aaatttgttt taagataatt tagttttaat gatatataat
    41761 attttgtatt atataatgta aattttataa tactattatt gttcagataa aagtctagga
    41821 gagtgaaaag atggaaaata attttacaac gagaactagt tttctagttg gagatgatgg
    41881 gatagaaaaa cttaataatt caaatataat agtatttgga gttggaggag ttggtagttt
    41941 tactgttgaa gctttagcaa gagctggagt aggcaatatt actattgtag attttgatga
    42001 tgtagatata accaatataa atagacaaat accagcccta cattcaactg taggaagata
    42061 caaagttgat gtcatggaag aaagaatatt agatataaat cctaatatca atataaaaaa
    42121 aataagaagt ctttataata aagatactag tgacgaaata ctaactgaaa gatatgatta
    42181 tgttgtggat gcgatagaca tggtaagttc caaaatacat ctaattgaaa cttgtgaaaa
    42241 aaaaggattg aaaataataa gttctatggg tatgggaaat aaattagacc caactaaaat
    42301 agtcgttaca gatatacaca aaacaagtac ttgtcctcta gctaaagtta tgaggaaaga
    42361 acttagggat agaggtatca aaaagttaaa agtagtatat tctactgaac aaccaataga
    42421 acttaagaag aaagtaatga atggtagaaa agtaacacct ggtagtgtat catttgtccc
    42481 atctgtagga ggtttaataa tcgcatctgt aatagtaaat gaactattag gacaataagt
    42541 tttaaatatt ttaatttata aattttatat acaaatttga aggctagaag gatgaatata
    42601 aagcaatttt atattcatac ttttagccta gttattttat attaccttat tttatgtagt
    42661 tgtagcttta ttttttagat tatgtatcta tttcataatt atattttgta aatataattt
    42721 ctccatcact ataaaaaaca atatctccta ttctgtctgt tctataaatt gatacatggt
    42781 tttgttttag attattaata acctccctat gtggatgacc atattgattt ttatatccac
    42841 atgatattgc agctatatct ggactaattt tttttataaa ttctaaagat gaagaagaat
    42901 tacttccatg atgacctatt tttataaaat taatattatt taatttaaaa gaatgtaaaa
    42961 tttctttttc attttcttct tcagcatcac ccataaataa aaaagaatta tcattaaaag
    43021 ttaatttgaa aactatagaa tttaaattag attcttcttg tatatatgat ggacttaata
    43081 catatatatt tatattgtta tctatgttta aaacatcatt tttatagaga tgctgaatag
    43141 ataagttttt atccgtacaa gaatttatta gattttgata agcctcacta tcagttgatt
    43201 gttcaggcat atatatggag tttacattaa atttttttat aatattatca agacttccta
    43261 tgtggtctga atctggatga gttgctatta taatatcaat agtttttatt cttttttgtc
    43321 taagataact aataattata ttttcgctat cttcatcacc tccatcaatt aatatattct
    43381 tatttgtggg tgtttgaact aaaatactat caccttgtcc aacatcaatc atatgtatag
    43441 atagtagaga ttttttgtca cagccgatta aaagtgatat tataataagt attgataaaa
    43501 aaattttatt tctcaataaa aaatcactcc attcattaca tgtttatata aatttatgat
    43561 gggtaatata agatatatat gacttattat attttatatt ttaaacaata taaatgaaat
    43621 taataccata ataaaattgg agttttttat atatacaaaa taaaattttt aaaattaagc
    43681 aataaaaaag gtaggaggat tttatgtttt tcaaagatat tgaactaaat tcaaaaaaag
    43741 agttagaccc atattttgat ttagtagatt atgaagcttg tgagtactgt tttagtactt
    43801 tatatatgtg gcaacatgtt tataagacag gatattatat aggtgaagat tttgccgttt
    43861 tagttgggga atacgaagga gatagcttct caatattacc acttgctaaa aaagataaac
    43921 ttccagaagt tgtagatttt gtccttgaat atttttctaa aaataataaa aaaatatact
    43981 taagaggtat tactactgaa gtagtagagt ttttaaaaga aaaatatcca ggtagatttg
    44041 agtatataga agaaagagat ttatttgact atatttatga tgcagaaagt ttaagaactc
    44101 tagctggtaa aaagaatcaa aagaagagaa accatataaa ctatttctta aaagagtatg
    44161 caggtagata tgaagctaaa ttattagata aagaaaactt tgatgagtgt ttagtactaa
    44221 tgaaggaatg ggaaagtaat aaggaagaaa ataatgaatt tgatgaaagt atggatgatg
    44281 aattaatagg tatcaaaaag atatttaacc attatgacat attaaaagat aaggtaaaag
    44341 tgtttggtgt ttatgtagat ggaaaattag aagcttttag tataggagag cttttaaatc
    44401 caaatatggc attgattcat atagaaaaag ctaatcctga tataagaggt ctgtatccat
    44461 ttataaatca acaatttttg gtaagtgaat ttaaggacgt tgagtttgta aatagagaag
    44521 aagatttagg tattgaagga ttaagaaaag ctaaactttc ttatcatcct tgcagatttg
    44581 ttgaaaagta cagtgttagg gaggcttaat agactatatg gagattaggt atgctaaaga
    44641 agaagagata gaaagtataa aagagatttg gagttattgt tttaacgata cggagagttt
    44701 tatgaagtac tattttaatg ataaatataa atctgaaaat actgtagtag ctttagatga
    44761 aggaaaaatt atttcttctc ttcagctaaa tcagtataag ttattactaa atagcaaggt
    44821 atataataca tcatatgtag taggagtttc tacacttcca gagggaagag gagctggata
    44881 tatgaacaaa gtaatgaaat ttactttaaa cgaattgtat aaaaaagggc aattagtttc
    44941 tatattgatg cctattgact atagacttta tagaagattt ggatatgagc attgttacga
    45001 ccagattgaa tatactatta acacagatga tttaaaaaat tttaagtcaa gtggaaaaat
    45061 gataaagtct aatttatcac aaattgatga tttgatacaa atagatagaa cttttttaaa
    45121 tgaggttaat ggtaatgtgt taaaagatga acattattat gaaaacttat ttaaagagat
    45181 tcaaagtgaa gatggatttt tatacattca tgaaggaaat gaaaaagatg gatatatagt
    45241 ttattttctt caggaagata aaatgtttgt cagagagtta ttttataaaa atatagatgc
    45301 ccttaaatca atgcttaaat ttatatataa tcacaatact caatgtaaga tagttactat
    45361 atctactcct acaatagata agataagatt tatattagac aaccctaaag atagtgatat
    45421 aaaaataaaa ccttttatga tgggaagagt tataaatgta aagaagttta ttgaagatat
    45481 agatattgag aatgatatta atagctcatt taatttatta atagaagaca agtttataga
    45541 tgaaaataat ggtctattta aaatttctat acagaataag aaggtatctg tagagcagtt
    45601 agataaaaaa ggtgcagaaa agcctcaaga agattttgat attaaattag atataaatac
    45661 cttaacacag ttatcatttt cttatataga tgtaaatgaa gctattttct taaatgatat
    45721 aaaagatgtt tcagaagaaa cactagaaac acttaattgt attttttcta aaaaaaataa
    45781 ctatataaat gaatatatat aggtagagta cgttaaaaga ttttaaaaat cttttgacgt
    45841 atttttttgt ttagataaca atttatcttg tttaaaaatt ttctatttga aaataatcta
    45901 aataatatac ttttatatgg gagtgattat aattcaaata agaagaccat tattaattat
    45961 attaatgttt gtggtagcta tatcatttat cattacaaat aacacaaaaa acgacgaaga
    46021 acttaatgat aagataataa ctatacaagg agttgtcaaa ggaaaaattg aaaagaaaag
    46081 atacaatcag tacaaggtag gggtttttct aataaatgat tatacaaaaa ataaaaattt
    46141 gaagattggt cagaaagtta atattacagg taaattcaaa agtttagata aaatgaagta
    46201 tgatgatttt gattatggaa ggtatataaa aagtactgga tataaaggta tagtgtatat
    46261 aaataactat aaaattattg ataaaaataa actttactct ttaatagggg aaataaagtt
    46321 ttatattagt aaaacttata ggtacttata taagaaaaac tcagatttta taaattctat
    46381 actattagca gaagttgaaa atttgactga ggaacaaaaa gaaattttta ctagaactgg
    46441 aactagtcat gtaatatcta tatcaggtct tcatacagga atattatgtg taattatatc
    46501 ctttctatta agaggtataa ataaattgta taaattatta atactgtcga tttttataac
    46561 tttatattgt ataatggtag gtgcgtctcc atccataatt aggtctatag catttgttat
    46621 gattttttat ttatcaatat ttattgatag aaagaaagat ggaatatcgg cattgtctct
    46681 aattgggata attttaatta tgaataaccc ctatgttata tataatgtaa gttttcaatt
    46741 gtctttttta gctacacttt ctatattaat attctataat aaaataaata gtattataaa
    46801 attaagtatg gtatcattga caatatcatc aaatatatta actttaccta taatttatta
    46861 tacatttaaa ggtataccat tgctttcaat tataggaaat ttaattatag taccatttgt
    46921 gggagtaata atgtatttaa gtattgcaag tttaatcgta tttaaagtaa gtgtagtaat
    46981 agctaaaatt atatcttttt ttaatagtac atttatagaa agcatatttt ttttactaga
    47041 aaaaatcagc aatttaagtt ttgcatacat taatatagaa aatcctaaat tttatattgt
    47101 tgttatttat tacataggag tgttttttta catattttac atagaaggaa aagagataaa
    47161 ggagcaagag aatgaattac aaggatatta taaggaatgt aaaagagaaa aactttgaaa
    47221 aaatgtatct tttttatgga agagagtatt atttaattga gaatgcaata aaagcattta
    47281 aagatagtct aaacgaggga atgcttgatt ttaacttaga tataatagat gggaaggaaa
    47341 tagtcttaaa tcagcttata agctctatag aaacacttcc atttatggat gatagaaaga
    47401 tagtaataat aaaagacttt gaactattaa aaggaaagaa aaagaatttt acagatagtg
    47461 atgagaaata cttaatagag catttagaca atattccaga caccacaact atagtttttg
    47521 ttgtatatgg agatgtagac aaaagaaaat cattagttaa gaaaatagga aacaatggaa
    47581 ttgtttttga ttgtgataag ctttctgata tggatttgtt taaatggata aaaaaatctt
    47641 ttgctttaaa tgatgttata atagataatt ctcaaatcat gtattttatt gaacaagaag
    47701 gatacagaga taagagtagt gaaaagacat tatctgattt agaaaatgaa ataaataaaa
    47761 taagttcatt tgttggtaaa gggaataatg taaccaatga tgtaattgat aaattatctc
    47821 aaaaaaaagt tgagaatgat atattcaaat taatcgatta tattggagaa caaaatgctt
    47881 ctaatgcaat gaaaatatta aatgatatga tacaagaagg cgaatctgta ttaggtatat
    47941 tttctatgat agcaagacaa tttaaaataa ttatgcaagt aagacagtta caacttgatg
    48001 gatattcaac aaaacttata gctgataaat taaaaatgca tcaatttgtt gtaggaaaag
    48061 cattaaagca aactaaaaat ttttctgatg atataattgt ggaaatatta aactatatat
    48121 tagaaagtga ttataaaata aagacaggtc ttataagaga tactttagca gttgaaatgt
    48181 tggttagtag atactgtaaa agagaagcaa tttaaaaaat ctatatatta ataaaaaata
    48241 aatagcaaga tatgttatag ttcagagtag atgtaagcta aattaagttt gatatacaat
    48301 caactataat atgtctaact atttattttt tattgtataa atttacaaaa taaaaaaccc
    48361 ctacataggg gttattatat ataagaaata taaactggta attaagcgtt cataccgttt
    48421 aatttttgag ctaatttagc tacttttcta gctgcagcat tcttatgtat agttccttta
    48481 gaagctactt ggtatattct tttttgagcg tattggaact tagcaacagc atcctctctg
    48541 tttccagctg caacagcatc ttcgaatctt cttattgcag tttttatttg agattttcta
    48601 actctattta aagcagtttt cttttcgata acacttattc ttttcttagc tgattttata
    48661 tttgccaccg tgttcacctc cctgtatttt tttatcatat tcatcgtcaa catattagat
    48721 tttaacagaa ataaatctaa aaatcaagga ttattattta tatattttaa taatgttttt
    48781 tattttgatt ttttagactg taaattgtgt ataaataatt tgcaatatct tgtttttaaa
    48841 ttggtatatt ttgtgtttta a
//
LOCUS       DAMJVK010000019        47990 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.019, whole genome shotgun sequence.
ACCESSION   DAMJVK010000019 DAMJVK010000000
VERSION     DAMJVK010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 47990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47990
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.019"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            126..812
                     /locus_tag="PC414_001081"
     CDS             126..812
                     /locus_tag="PC414_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2463489.1"
                     /translation="MSSILLVEDDLSLIYGLEYSIQKNGFSVDVARTVEEALQIYKEK
                     NYDLLLLDVSLPDGDGFDICKRVREASNVPIIFLTASDEEVNVVMGLDMGGDDYITKP
                     FKLNELISRIKALLRRYNFTSENVTELKSNNITVKLLENRVFKNEIELELTTAEYKLL
                     CLLMKNKNIVLTRKNILDKLWDGNGSFVDDNTLSVYVRRLRNKIEDNPENPKNLLTVR
                     RMGYKWNVIK"
     gene            823..2064
                     /locus_tag="PC414_001082"
     CDS             823..2064
                     /locus_tag="PC414_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2463490.1"
                     /translation="MKVFSNKDIKIFFLVIISVLLMFIVVGQIVVINITNDYKSALLE
                     HDYNIAGYLNSMGVDKSKIPAVFTTNEDKYTLKGKEILNTARYDFGTDDSFMPSVKLF
                     QGKYIKTMFLYLFVFFTMIIFILYVYFFRQQNKIEEASYKIDSFMNGNINVRLNTYEE
                     GSLSKLFGSIDAMSTSLNTHILKEKQNKEFLKNTISDISHQLKTPLTALMMYNQILQE
                     ESHNSEIVENFVQKSQNELERIESLIQNLLKITKIDSKTIILNKASVNVKKLITKILY
                     SFETRAEKEGKSISIRGLENTNLFCDYEWISEALSNLIKNALDNTKENDKIMIEWVET
                     PITTIISVSDTGNGIHIEDIHHIFKRFYRSKFSKSNQGLGLGLPLVKSIIEQHNGTIT
                     VESNFLQGSTFTLSFLKLTNM"
     gene            2135..2818
                     /locus_tag="PC414_001083"
     CDS             2135..2818
                     /locus_tag="PC414_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2463491.1"
                     /translation="MDILVVNNLCKTYGNGYTKVEALKDVSFTVSKGEFVTVVGESGS
                     GKSTLLNLLGGLDTPTSGKIFVDGKEICSMKEEKQTIFRRRNIGFIFQSYNLIPELTV
                     EQNIIFPLLLDYKKPDTIYLDEVLTILGLKDRKSHLPNQLSGGQQQRVAIGRALITKP
                     SIILADEPTGNLDSKNSSDVISLLKVTAEKYNQTIIMITHNTNIASTADRILQVSDGM
                     LKDLGVNRE"
     gene            2815..5148
                     /locus_tag="PC414_001084"
     CDS             2815..5148
                     /locus_tag="PC414_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2463492.1"
                     /translation="MRSYLELVPQYAKVHKKKNRTTILCIVIAVCLVTAIFGLADMAI
                     QCQKVQFIKDDGNWHIVLKNLNEEAISEISSRVDIAHYGRGQVIDNGEFKSKEILIQG
                     LDEDLAKQLGLEIIKGRYPVVENETLIDVDAVKSFNLKVGDFIKIKLADGRNKNYKIV
                     GEYSSLSRLRASDKHGLMLSILGTQAIKKSETYDYYYITFKDKVNIRKATSDIKNTFN
                     LSDKQIGKNEKLLSIIGQGDSDYSKPLYLTAGGLFILVLIASIIMIYNSFNMGVIERI
                     KFFGLLRCLGASKLQVKKFVVLESLILSVKAIPLGLLLGCIVTIISSIFLKYVNTELF
                     GSMPILKVSFIGIVFGIIVGFLTVILSAIVPAKKASGVSPLSAIRGNLMFKEILTSGK
                     ASKIANAGSSKIDVSMGINHAFSRKKSFILMTSSFAISIVLFLGFSVFIDFMYQALKP
                     IKPEAAHISIVDADFKSVLTKDDINEISNIKGIKKVYSRMMTDVEATYNNKEATSQLI
                     SYEKNQFDWAKKYLVKGEINEDKLESENIGLVEEGHGFNIGDKIYIKNGNSKKEIEVA
                     GILSSISFDVKDKYIGNIITSENTFTSITDIKDYIIIDTQVDEDAPEDLIQNIRNVLD
                     DNLEVRDKRQGNLEAKNSFYTMAVFVYGFVFIIAIISMFNIINSMNISVTSRINYYGI
                     MRAIGMSNKQLRKMVIVESSTYAVSGCLLGSVLGLILHRYIFVSLVTLKFHIDWQIPF
                     DLLFIIVVTMIIITLLAVRKPIKKICELDIIEVVNAQ"
     gene            complement(5256..5818)
                     /locus_tag="PC414_001085"
                     /pseudo
     CDS             complement(5256..5818)
                     /locus_tag="PC414_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5932..7281)
                     /locus_tag="PC414_001086"
     CDS             complement(5932..7281)
                     /locus_tag="PC414_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF2463493.1"
                     /translation="MEKAKKLGLFSMILLGINSIIGSGIFLLPGKVYNLAGQNSMFIY
                     IFATLLVLSILLCFAEVGSMFDKNGGAYLYSKKAFGDFIGFEVGTMSWVIRIISWSTL
                     AVGFATALGSFWPESATEYKGYIAAILVTLLSINSLFGIKSTKIMNNVITIAKLVPLI
                     VFIIVGIFFIKFVNIVPSGNVVNSSMGPAIILVFYAFTGFESFIVASGEMENPKKNLP
                     VALITTIFICAIIYILIQIVCMGILGDRLFENSIPIADASSVFLGNYGKFFISVATLI
                     SIFGINIGSSIVTPKCGSSLAEEGSLPAFIGKTNKYGAPYVAIIISLICCIPLVLTGS
                     FEQLAVMSVIARFAQYIPTCLSVIVLRKRTDVKASFKIPFGPVIPMVAILGSLWLLQQ
                     AWAEDISKPITQNRVLIGLGAMLLIAPLYIFMKKNKEIEHKNKSEDSKLYISREKIQ"
     gene            complement(7318..8538)
                     /locus_tag="PC414_001087"
     CDS             complement(7318..8538)
                     /locus_tag="PC414_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2463494.1"
                     /translation="MQQALKLKYQTKELGKDFVIESCNSIKPWLINIRRELHKIPELA
                     LEENLTKQKVISYLKEIGIDYMEFTKHNGIMAYILKESADKTICIRADMDALPIEEEN
                     NIPYKSIHSGKMHACGHDAHTTMLLGACKVLHSIKDKLNVNVKFLFQPAEEGFGGAKF
                     LVEDGCLENPKVDYIFGLHVMPHIETGFIEIKYDTLNASVDTIKISIKGKRAHGAYPE
                     NGIDAIVTASQIVTSLQTIISRNLEPNNAAVLTIGKIYGGDAHNVICEDVKLEGTLRT
                     LNSKTRNFMIDKIAKIVGDTASAFGCVGTLHVSDENYPAVINEKELVDTVISNTKELL
                     GEEKFILRPNPSLGGEDFSFYTEHCKGAFFHLGCKNEEKGLISPLHTSSFNIDEDCLP
                     IGVMMHVMNTLYFN"
     gene            complement(8907..10919)
                     /locus_tag="PC414_001088"
     CDS             complement(8907..10919)
                     /locus_tag="PC414_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2463495.1"
                     /translation="MKKSVALVNDSRKDLIDFLENNLKLVFGDSININRYFINEINDN
                     DIINDDVILVMSVERLDKIINNILDKKKVIVVRRTFREDKIYNLLSLPQGTNVLIVND
                     SDETTLETISLFYKIGVTNIRPIPYMNDNNYKNIKIAITPGVPEKVPSFISDIFDLGH
                     RYIDISTFIEIINLLQIDSKEIQSNLVKYSEEIISLDTGIKDKYKELFLKIEELDTIL
                     NLSKDGILFTSKDGEINTYNSKVKDILDINEDIYGKYIEDIFVDSLKVLLSEKEILDK
                     VVVFNKKYINVNKKNIYNRDEKMGTYYSLQEITYIKKLEQNLTKKLREKGQIAKYTFK
                     DIKTNSPKMFECIDLAKKVSKSDLSILIRGESGTGKELIAQSIHNNSNRKNQPFIAVN
                     CAAVPENLLESQLFGYDKGTFTGGLKDGKQGLFELANNGTIFLDEIGDMPLELQTKLL
                     RVLQEKQIMPVGSHNVINIDVRIISATNKNLEQMIDNSQFREDLYYRLNTIPINIPPL
                     RERKEDILIIMEDLINKKLVITPEAKKLIQNYMWKGNIRELQNVTSYLNIMCEDIVLE
                     KDLPPNLRSSDNKNTSLKLKYSKNDILNILEILILNKESDVGIGRGLILKALLDKNLQ
                     ITEGKIKKIFEYLKKEELIICSSGRYGSKITQKGEDFYNKLKYKGL"
     gene            11390..12040
                     /locus_tag="PC414_001089"
     CDS             11390..12040
                     /locus_tag="PC414_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HDF2463496.1"
                     /translation="MKAEKTSIYPIIKNNLNKISIVEYKKGQRFVASDKDLQEVFFII
                     EGVALVECTTRGGNKFLVDIVPENEFIGKISYIYEHNLKCDIFAKTNIKLFRFEKNVF
                     EEFYLKPDFIALFHRKCTRRIYELYKTRMVRELFSCTEVIAYCISNKQENNVCNIKAI
                     RNFSEIYSISRKSRYHSLLKLTEKEIVKKMGNTYEILNYDELHKLAFEVKEFLEDE"
     gene            complement(12180..13556)
                     /locus_tag="PC414_001090"
     CDS             complement(12180..13556)
                     /locus_tag="PC414_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2463497.1"
                     /translation="MQELFSLKDENKRFYKILLSLCIPIIIQNLISTSVNVIDTIMIS
                     SLGETSVASVGVANQFFFLFNMSLSGITGGAGVFISQFYGKKDVSNIRKVTGLTCVLA
                     IVLSFVFVIPALLTPKPIIHIFSYDSEVVKLCIDYFSIAVFSYPLIAVSTVFSTGSRG
                     VRNPKLGMICSAFALVTNVILNYGFIFGNFGLPALGVKGAALATVIARICELILMITY
                     VYLYKKDYILKFGLKNLKAIDKIFIKSFSSKSFPIFVNDSVWAIGTVLYSVAYARAGT
                     SAIAASQIATSTGNFFIMTAVCIASGASIMLGNELGADHIKRAIEYAKKFSILVFSAG
                     LILGIILILNIPLLLKMFSVSDSLASDITKIFFIMGILMALKSFNTLVIIGILRSGGD
                     TKYALFLELGCMWLASIPLTFIAAFKGAPIFVLVLLTYSEEVVKFIFGVPRALSKKWA
                     INIVKEID"
     gene            complement(13572..15485)
                     /locus_tag="PC414_001091"
     CDS             complement(13572..15485)
                     /locus_tag="PC414_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDF2463498.1"
                     /translation="MIVLSCNNLNKSFGIDSILENISFTVNEGDKIGIIGVNGTGKTT
                     LFKIISGIYGYDSGDIYTSKDCEIGYLEQNTNFYSDNTILEEVLEVFKNLIEMESYLR
                     ELEVKISEESTKTNSPIIEKIMDEYSHKLELFSDLNGYGYKSEAKGVLKGLGFSDNDM
                     DKPISILSGGEKTRVLLGKLLLKKPTLLLLDEPTNHLDSEAIEWLEVFLKQYKGTVML
                     ISHDRYFLDQSVNRIFEVHNKKLKVYNGNYSKFVELSKIEKELELKKFEDQQKEIKKQ
                     EESIERLKAYGREKHLKRARSKEKALDKVDVLDKPEAYRKKARIQFTPSVQSGNDVLQ
                     IRDVSMGYGERILFKDLDLDIYRGEKVALIGANGVGKSTLFKIITNELQPLSGNIKFG
                     TNVHVSYFHQEQKTLNLDNTIIDEIWENNTHLTQTTLRNMLGAFLFVDEEVFKKISTL
                     SGGERARVAILKLILSNANLLLLDEPTNHLDIDSKEVLEEALTNYDGTIFTISHDRYF
                     LNTVVDKILVLDENGITEYLGNYDYYIDKKRQIQEMSIIEEKEEKTRTQIKDEKRKER
                     EQREIEKKNRIKRQNIEKEIEKLEIEIEKLDILLCQEEVYSNPDKAKEVSQEKINLEN
                     NLASLYDEWEEFM"
     gene            15621..16253
                     /locus_tag="PC414_001092"
     CDS             15621..16253
                     /locus_tag="PC414_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HDF2463499.1"
                     /translation="MLGNKNISMAVIRRLPKYHRYLGDLLDRDIQRISSKELSDIIGF
                     TASQIRQDLNNFGGFGQQGYGYNVEALHTEIGKILGLDRPYNAVLVGAGNLGQAIANY
                     AGFRKAGFEIKALFDANPRMIGLKIREFEVLDSDTLEDFIKNNNIDIAVLCIPKNGAQ
                     EVINRVVKAGIKGVWNFAPLDLEVPKGVIVENVNLTESLFTLSYLMKEGK"
     gene            16392..16931
                     /locus_tag="PC414_001093"
     CDS             16392..16931
                     /locus_tag="PC414_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HDF2463500.1"
                     /translation="MVFVEELWKKAIEFHGHECPGLAIGFKAALAAREYLGCKQSGDE
                     EIVCISENDACGIDGIQVVLSCTMGKGNLLLRMTGKSVYHFFNRENGKSIRIYVKSKP
                     FQMDREGYKKYLLESDFEEIFEITDTKLRLPEKARIFKSLKCEVCGEFASEQYIRIEE
                     GKKVCLDCFNKYERFYEEY"
     gene            complement(17107..17277)
                     /locus_tag="PC414_001094"
     CDS             complement(17107..17277)
                     /locus_tag="PC414_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2463501.1"
                     /translation="MAYKITDACISCGACEAECPVSCISAGDDAYVIDAGSCIDCGSC
                     AGACPVDAPQPE"
     gene            17562..18629
                     /locus_tag="PC414_001095"
     CDS             17562..18629
                     /locus_tag="PC414_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HDF2463502.1"
                     /translation="MKNKLVYICIMSLLLFVVGCSNNPNKESTIKKDSKNNQSQVKEE
                     KREPVKKDITISFAGDVTMGNYKGSSYYGSFDHEFKRQGEDYNYFLKNVKPVFEKDDL
                     TVVNLEGPLTTASNAKEKKFAFKGKPGYVNILKSGSVEAVSIANNHSEDYFEEGMKDT
                     QFILDENKINYFGLGKDAVVDVKGIKVGLLGYNGLSTEYNEKNLKQMEDDIKSLKKKS
                     DVVVVYFHWGIELDYYPDKKQKDFAHYAIDKGADLVMGSHPHVIQGIEKYKNKYIAYS
                     LGNFCFGGNKNPSDTDSYIYQQTFTFSDNKLTSIKEPNIIPTSITSSNSRNNYQPKIL
                     DGSEKDRVLNKLEKISKDLNK"
     gene            18799..20700
                     /gene="cooS"
                     /locus_tag="PC414_001096"
     CDS             18799..20700
                     /gene="cooS"
                     /locus_tag="PC414_001096"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HDF2463503.1"
                     /translation="MACNTCKMCESADKKLESFVASKDVETAFHRTEDQKVKCGFGLQ
                     GVCCRLCSNGPCRVTPKSPRGICGADADTIVARNFLRAVASGAACYLHVVENTAKNLK
                     NVGITKGVVKGEKTLNQLAEMFGIESDEKYDKCIKVADKVINDLYKSRDDKMELVEKI
                     AYAPRVKKWKELGIMPGGAKSEVFDAIVKSSTNLNSDPVDMLVNCLNLGISTGLYGLT
                     LTNLLNDVMLGEPVIRMAPVGFNVIDPDYINIMITGHQHSTFANFQDRLKDEDVIKLA
                     QSVGAKGFKLVGCTCVGQDLQLRGEHYQEVFAGHAGNNFTSEAVLSTGAIDIVLSEFN
                     CTIPGLEPIADKYKVKMVCLDDVAKKANAEYIGLDRSKLDELSNTLIEKALESYKERR
                     GSIEIDIPKDHGFEQSLTGVSEKNLKAFLGDSWKPLINLIAEGKIKGVAAVVGCSNMT
                     AGGHDVNTVELTKELIKKDIIVLSAGCSTGGLENVGLMSPGAEELAGENLKEVCKTLG
                     IPPVLNFGPCLAIGRLEIVATELAAELGIDLPQLPLVLSAPQWLEEQALADGAFGLAL
                     GLPLHLALPPFITGGKLVTEVLTEKLKDLTGGHVIVNPDPKSSANQLEEIIIDRRKNL
                     GLKDVECNA"
     gene            20693..21166
                     /locus_tag="PC414_001097"
     CDS             20693..21166
                     /locus_tag="PC414_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF2463504.1"
                     /translation="MHRIFINKDLCTGCKSCVLACMLKHNKDYDMYTLDLENIDNDSR
                     GHIELDSKHNNPVPILCRHCDEPECVLACMSGAMHKDSESGIVSYDEEKCGSCYMCVM
                     SCPYGLLKPDDRSKQNILKCDLCKDEEYPRCVANCPSGAIELQKEENDELCSVRG"
     gene            21141..22367
                     /locus_tag="PC414_001098"
     CDS             21141..22367
                     /locus_tag="PC414_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDF2463505.1"
                     /translation="MNYVVLGASAAGINAVKTLRELDKDSNIVVISKDENVYSRCMLH
                     HVISEHRILKQINFVDEDFMEQNNVKWIAGKTVKGIDINKKVVQTEDITVNYDKLLIA
                     TGASSAIPPIKNLRDGNFVYSVRNIDDIYKIKEKAQNSKNVVIIGAGLVGIDALVGLF
                     KYEQLNISVAFMEKYILDRQLDEYTASVYENKFKEKGVKFYPSASIQEIVLDNSKNVT
                     GVAFSNGEVLDADMVIVATGVKPNADFLDGTGIEYDRGIIIDDMCQTTQKDIYAAGDV
                     VGKNAIWPLAVKQGIVAAYNMVGKDKKIEDEFAFKNSMNFMDIPTISIGMNTPVDDSY
                     KVLTRHGINDYKKFVFKDNVIYGAVIQGDISYVGVLTYLIKNKVEIYDLENRIFDICY
                     ADFFNIKENGEFCYSV"
     gene            complement(22506..23693)
                     /locus_tag="PC414_001099"
     CDS             complement(22506..23693)
                     /locus_tag="PC414_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF2463506.1"
                     /translation="MNITKNFLKDYLEKFGETPFLVRLKDEEEFLIGEGSPQFKVIVN
                     NDISKSELLKSTSLALGEAYINRDIEVEGDLFLTLNLLLKQIDKFNIDNSAVKHLLHS
                     SNSMRHQKKEIHAHYDIGNDFYSLWLDKTLSYSCAYFRNDNDTLYDAQVNKVHHILTK
                     LNLEEGMSLLDIGCGWGFLLIEAAKKYKIHGVGITLSEEQFKEFNARIKEENLQDYLE
                     VRLMDYRELKKSDLMFDRIVSVGMLEHVGRENYELFIKNVNAVLKDGGLFLLHYISGL
                     QESYGDPWIKKYIFPGGVIPSLREMIHICSDYKYHTIDVESLRLHYVKTLLYWQKNFS
                     ENLPKIKEMFDDKFIRMWDLYLCSCAAAFNTGIIDIHQILFTKGIKNDLPLTREYMYN
                     CSK"
     gene            23966..24790
                     /locus_tag="PC414_001100"
     CDS             23966..24790
                     /locus_tag="PC414_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2463507.1"
                     /translation="MEVYALVGSSGTGKSFKALEFAYENDIEYIIDDGILIYKNKVLA
                     GISAKQANTTIEAVKRAIFYNLEHRQEVREAIKKENIRKILIIGTSKKMVNQIVERLS
                     IGRVCKFINIQDISTKYEIEIAKQARKEGNHVIPVPAVEIKSMASGLSINSLKRLFRK
                     GSNRNMVVLEKTIIRPTFSYIGKFYISADVIKQIIEYEVYNFGSIDRINKINIENCNN
                     FMNIFVSININDLETIKKIEDIQQTIKKSIEKMTLVNVQKIDINIHKIKKINFLQC"
     gene            24919..26184
                     /gene="gluD"
                     /locus_tag="PC414_001101"
     CDS             24919..26184
                     /gene="gluD"
                     /locus_tag="PC414_001101"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-specific glutamate dehydrogenase"
                     /protein_id="HDF2463508.1"
                     /translation="MSGKDVNVFEMAQSQVKNACDKLGMEPAVYELLKEPMRVIEVSI
                     PVKMDDGSIKTFKGFRSQHNDAVGPTKGGIRFHQNVSRDEVKALSIWMTFKCSVTGIP
                     YGGGKGGIIVDPSTLSQGELERLSRGYIDGIYKLIGEKVDVPAPDVNTNGQIMSWMVD
                     EYNKLTGQSSIGVITGKPVEFGGSLGRTAATGFGVAVTAREAAAKLGIDMKKAKIAVQ
                     GIGNVGSYTVLNCEKLGGTVVAMAEWCKSEGSYAIYNENGLDGQAMLDYMKEHGNLLN
                     FPGAKRISLEEFWASDVDIVIPAALENSITKEVAESIKAKLVCEAANGPTTPEADEVF
                     AERGIVLTPDILTNAGGVTVSYFEWVQNLYGYYWSEEEVEQKEEIAMVKAFESIWKIK
                     EEYNVTMREAAYMHSIKKVAEAMKLRGWY"
     gene            26397..26987
                     /locus_tag="PC414_001102"
     CDS             26397..26987
                     /locus_tag="PC414_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HDF2463509.1"
                     /translation="MHFKSKDLNLKYLVLFLKIVYWFNPFIYIMEKYMDLDCELYCDE
                     RVLKNCTVQQKQEYALTIVNSMKRSTNFSNKFVAGLNKQSDIRKRVINMFSEKHKNGI
                     LRALIISLFSSITFFKFDSTSIIEENDFLERSRYTFEGRQEDMFVTIDFIYADVPIKL
                     RKEHEENCRVNGIVPKDSDSIEIIPRFYKDCMDLKK"
     gene            27318..28451
                     /locus_tag="PC414_001103"
     CDS             27318..28451
                     /locus_tag="PC414_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M56"
                     /protein_id="HDF2463510.1"
                     /translation="MSNFILNGIFRTVIVSSLSILMILIFKKNVFKRFSKKFNYYIWM
                     IVVIKLFLPFTYYTFTINILRSKKHININNINLEGFNNVSTISNNIILYTWIITVVVY
                     LIYTIFKYIKLKNLINDLSYDVDDGEIVNLYKNILEEFNITKDIKLKYSYEVETPAFF
                     NSCVLLPPHEYKLKELDWIFRHELMHFKSRDLYLKYVILFLKTVYWFNPFIYIMDKHI
                     DLDCELYCDERVLKNRNSEEKKDYALTILNAMRKGSNTSNKFIAGLHKQSDIKKRVMN
                     MFNEKYKNGILMALTLCLLSSITFLKVNSTSIVNLNILPKGMEEKDVTNSAIVTMIEF
                     TYAEAPKSMREEHKINCEFLGLIPKDSDKIGVGIEYYDSLLND"
     gene            29367..30389
                     /locus_tag="PC414_001104"
     CDS             29367..30389
                     /locus_tag="PC414_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDF2463511.1"
                     /translation="MKKKILIPVFASVMALSVSSIVNADEVNDSSQNKDDKTNTELNL
                     GEYKEVKYKVAKIKDGVAIKIREEGQVQNIAYSGDEFTVLGTQGEWVKVKVEDGEGWL
                     ATRYVDISEGVGYTNADKVNLRKDKSESSEVIEELEKGSSLLVLEDNGDWLKVKDGET
                     EGYVKSSYISDKAPVIEEPQVNPSIDQNVDSTNNNQQNNANTNQQNNNSNVPTANSNA
                     VQAVLNLAYSKQGCPYVWGAEGPNTFDCSGFTQYVYRNAVGKNIPRTSKAQSKYGQTV
                     SKANLQPGDLVFFTTNGSGSVSHVGIYVGGGNMIHSPSTGKTVSVTSINSSYYTARFV
                     TAKRIL"
     gene            30685..31605
                     /gene="pyrB"
                     /locus_tag="PC414_001105"
     CDS             30685..31605
                     /gene="pyrB"
                     /locus_tag="PC414_001105"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HDF2463512.1"
                     /translation="MLKSRNLIQPEDFSIEEIDEILELAQKIIDNPSKYSRICEGKLL
                     ATLFYEPSTRTRLSFESAMNRLGGRVVGFSEPNSSSASKGETLGDTMRIVSGYVDIIA
                     MRHPQSGAASEAARYTEVPFINAGDGKNQHPTQTLTDLLTIKSLKGTLESHTIGLCGD
                     LKYGRTVHSLVKAMARYKNTKFVFIAPEELKMPDYIKEAIKGHAYYETNNLDDVIGSL
                     DVLYMTRVQQERFEDKSEYERLKNYYILNKAKLEKASKDMLVMHPLPRVNEIDIDVDS
                     DDRAVYFKQAKYGMYVRMALIIKLLGINED"
     gene            31644..32336
                     /locus_tag="PC414_001106"
     CDS             31644..32336
                     /locus_tag="PC414_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HDF2463513.1"
                     /translation="MYKILENIYIGEDMYRMKVKGNFEGKMGQFYMLRAWDTYPVLSR
                     PISIHDIDEEGITFLYKVVGEGTQILSNLKVNDNIKLEGPYGNGYAKVDGKVALVGGG
                     IGVAPLYLVAKNIKNCDAYLGFREDVILEDEYKQVCNKVYTTVGNTFVTDIIDVEKYD
                     YILTCGPTPMMEKLVKMVEGTKTRIMVSLENHMACGVGACLVCTCKTNGGNKKTCKDG
                     PVFWGEDVIFNG"
     gene            32329..33231
                     /locus_tag="PC414_001107"
     CDS             32329..33231
                     /locus_tag="PC414_001107"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HDF2463514.1"
                     /translation="MGNLSVKFGSVEFKNPVIMASGTFGFGKEYNEIYDIQKLGGISS
                     KGLTLNKKPGNNGMRVHETSSGMMNSVGLENPGVQGFIDYELPFFSKLDLVRIANVGG
                     GTLEDYLLGVQMLNDKPIDIIELNISCPNVKAGGMAFGIKNEVAREVVREVRNITKLP
                     LVIKLSPNAEDIVGMAKVCEEEGADGVSLVNTFKAMAIDIKNRRPVFENVYAGLSGPA
                     IKPIALRMVHEVCKNVNIPVMGMGGITKATDAIEFIMAGATCIQVGTANFMNPRIGIE
                     IIDGINEFMDREGIKSLDEIRGII"
     gene            33316..33900
                     /locus_tag="PC414_001108"
     CDS             33316..33900
                     /locus_tag="PC414_001108"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HDF2463515.1"
                     /translation="MSKVDVVDILKKSDALLEGHFLLSSGKHSNRYVQCAKVLRFPEY
                     AAEVLSTVVEQIKDLDIDLVVGPAMGGVIVSYELGRQLGKEAVFTERKDNTMELRRGF
                     EVKKGAKIIIAEDVVTTGKSTMETKRVLEALGGEVVGVACIADRTNHDIGMPIYSAIK
                     LDIQVYESDECPLCKEGKLPVVKPGSREFKELGM"
     gene            34114..35040
                     /locus_tag="PC414_001109"
     CDS             34114..35040
                     /locus_tag="PC414_001109"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDF2463516.1"
                     /translation="MKNQLNENHKKVVFFGVGAVGATFAEQFFNSKYDFKILCDNERK
                     KRYLEEGFIINGKRYDFDYVTKDEYKQEADFIIIGLKYNNLKENIKELDGLVGKNTVI
                     MSLLNGVDSEEIIGERFGIEKMVYSYVTNIDAKKINNNIIHTTNGIIVFGNKDNSEDR
                     KTNIITEVFDDVNIEYTLSKDIQRDMWWKYMVNIGVNQTSAILGAPYGVFQSSEHLRE
                     LAKSAMREVVAIAQAKDISLTEDDVEHSLHRILEHSKEGRTSMLQDVEAHRLTEVDMF
                     SKNICKLGKKYNIPTPINQTFFYMIKVIESRF"
     gene            complement(35092..35982)
                     /locus_tag="PC414_001110"
     CDS             complement(35092..35982)
                     /locus_tag="PC414_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2463517.1"
                     /translation="MFEELKTFVAVVEYKNFTKAGEYLNLSQPSVSKHIKNLENYFKV
                     VLINRSIKQKTIFITESGQILYKRAKEILNLLNITYHDVSQVSDAITGHLKIGASLTI
                     GEYILPNFLALFSKKYPDIDVEVFIKNTSIVSSHVKDYILDIGLIEGTCSSPSFIQEY
                     FFEDKMVLALPYKSHLLKDFSFDKLQNQKWIVREDGSGTRDYLDMFLSVKEIIPKSMM
                     VFGSNYAVKESVRNNLGITIVSNLVTSLPVLNNELSVIELGSSYNRHFSYIFPKDITL
                     SKAATIFIEELKIFSNLNSI"
     gene            36170..37201
                     /locus_tag="PC414_001111"
     CDS             36170..37201
                     /locus_tag="PC414_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HDF2463518.1"
                     /translation="MLKKKSSLTLKTIREILPGLFVSVLVGYISIFISILIPKVGAAS
                     ISIFLGMFVGNLFLNQKVFQKGYKFSETDLLSYSIVLLGATLSVSTLIDLKVSGILFI
                     ILQMTITIIAALYIGKKLGFEENFRFLMASGNAVCGSSAIGATVPVVNATDKEKGIAV
                     TIVNVTGIFLMFLLPIISQFLYSHELVKTSAMIGGTLQSIGQVVASGAIVGENVKDLA
                     TIFKIVRVIFLVVVVLVFGHMKNQSNREILEEEKDEIKKKKVTIPWYVIGFFITCALF
                     SMNIIPKEVSVLCKEISNKLEIIALAAIGLKVNVKDLVKQGKEVSLYGLFVGIVQVVS
                     AVVLIKIFI"
     gene            37495..38370
                     /locus_tag="PC414_001112"
     CDS             37495..38370
                     /locus_tag="PC414_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HDF2463519.1"
                     /translation="MNVYEKGNGVILEGVTDFDPVHIFECGQCFRWHKQEDGSYTGVA
                     KGRILNVKKENDKIYLNNTNLKEFNSIWYNYFDLGTDYTEIKNKLKNMDEYLNKATEF
                     GWGIRILRQDGWEMLISFIISSNNRIPMIQRAIENLSRKFGKYIGEYEGNEYYAFPTP
                     EELNKASQEEIRACQTGFRDKYIKSTTQAVIENNDEVSEYTNLSTEDCRKELLKFNGV
                     GPKVCDCIALFGMQKYDSFPVDVWVKRVMQEFYIDEDMSLPKMRTYGIDKFKEMSGFA
                     QQYLFYYARELGIGK"
     gene            38388..39878
                     /gene="cls"
                     /locus_tag="PC414_001113"
     CDS             38388..39878
                     /gene="cls"
                     /locus_tag="PC414_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDF2463520.1"
                     /translation="MGVIGTIFLFYLIISYLAGAIISVIILLENRDPAKTMSWLLMFI
                     IFPGVGLMIYAISGRNIRKRKLFKTQKLANNIKEKKLFDTLEKITEIVELEKESIKQN
                     KLLRDEEDGSYRKRVINMLLKTGMFPFTKNNKVDVFVDGNEKFKRLIEDIREAKDHIH
                     LEYFIIKDSEIGRVLKEELIKKAKEGIKIRILYDDVGCWRFWFNRKFFREMREVGIEI
                     AAFLPTKFPIIGGKLNYRNHRKIVVIDGIIGYTGGINIGDEYLGKNDKFGYWRDTHIR
                     IKGISVYMLQMTFLIDWYYTTKEVLVTKNYFPSVGNVGESMIQVVASGPDSDWEDIHY
                     AYFSAICQARKNVYIETPYFIPDESLLKAIKSAALSGVDVRIIFPKIADHKIVNIASY
                     SYFEEILRAGGKVYLYNKGFIHSKVVIIDDKIASAGTANMDLRSFMLNFEVNAFIYDE
                     EVIRVMTDDFFEDLSHCEELNLEVFKNRNIIQKIKESVARLFSPIL"
     gene            40071..40355
                     /locus_tag="PC414_001114"
     CDS             40071..40355
                     /locus_tag="PC414_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDF2463521.1"
                     /translation="MKIRPLADRVVIKKVEAEEKTASGIVLPGAAKEQPQIAEVVEVG
                     PGGIVEGKEIKMELTVGDKVIFQKYSGTEVKIEGQEYTILRQSDVLAVIE"
     gene            40388..42016
                     /gene="groL"
                     /locus_tag="PC414_001115"
     CDS             40388..42016
                     /gene="groL"
                     /locus_tag="PC414_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729227.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDF2463522.1"
                     /translation="MAKEIKFSEETRRALEAGVNKLADTVKVTLGPKGRNVILDKKFG
                     SPLITNDGVTIAKEIELEDRFENMGAQLVKEVATKTNDVAGDGTTTATVLAQAIIREG
                     LKNVTAGANPILLRKGIQKAVTVAVEELKNQSRIVETQEAISQVASISAGDEEVGKLI
                     AEAMEIVGKDGVITVEESQTMNTELDAVEGMQFDRGFVSAYMVTDVDKMEAVLNDPYI
                     LITDKKISNIQELLPVLEQIVQQGKKLLIIAEDVEGEALSTLVVNKLRGTFDVVAVKA
                     PGFGDRRKEMLQDIAILTGAQVISEELGYDLKEADLSMLGRASSVKVTKESTTIVDGS
                     GDKKAIEDRVTQIKHQVEQTTSDFDREKLMERLAKLAGGVAVVKVGAATEVELKERKL
                     RIEDALNATRAAVEEGIVAGGGTAFVSVIPAIGTLIESLEGEVKLGAQIVKKALEEPL
                     RQIAINAGLEGAVIVQNVVNSEAETGFDALNEKYVNMIEAGIVDPTKVSRSALQNAAS
                     IASTFLTTESAVADLPEKEDAGMPGMGGGMPGMM"
     gene            42278..43018
                     /gene="larB"
                     /locus_tag="PC414_001116"
     CDS             42278..43018
                     /gene="larB"
                     /locus_tag="PC414_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HDF2463523.1"
                     /translation="MDLRKLLEEVKNENIDIDFALEKLKDLPYEDLGYANIDHHRELR
                     NGYPEVIYCEGKSDEHILGIIDKMNQKSSNILGTRCRKETFLKIKEIYNHAEYEEASK
                     ILKIQNHDIENIGKGKIVIATGGTSDIPVADEAYHTAKFLGNDVDRIYDVGVAGIHRL
                     LNKRHKIDSARVIVAVAGMEGALASVIGGLVDVPVIAVPTSVGYGANFGGLAALLAML
                     NSCASGISVVNIDNGFGAGYLASMINKL"
     gene            43102..43836
                     /gene="larC"
                     /locus_tag="PC414_001117"
     CDS             43102..43836
                     /gene="larC"
                     /locus_tag="PC414_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HDF2463524.1"
                     /translation="MSEKILYFDIISGISGDMTLASLLNLGVPKEIFLEEIHKLRMSD
                     EFNINISSKTENGIVGTKVEVITKEKHTHRNLVDIFEVIDKSTLNENVKSKAKKIFMV
                     IGEAEAKVHGTTIDKIHFHEVGAIDSIVDIVGASILVDLLCVDKVCASTVSVGSGFVK
                     CEHGIIPIPAPATIEILKGVPIKLNNVNGECTTPTGAAIIKVLCDEFVDELEFSPKQI
                     GYGIGHKKFEVPNILRTVLGEKKKKN"
     gene            43866..44285
                     /locus_tag="PC414_001118"
     CDS             43866..44285
                     /locus_tag="PC414_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF111 family protein"
                     /protein_id="HDF2463525.1"
                     /translation="MSSEIYSYLYEKILDEKALDIYTESIYMKKNRPATKVSILCSES
                     DLEKFIKILLLETSTFGVRYNAYNREILERKFVKLDTKYGVVTVKLGYYKGKLVKATP
                     EYNECKTIAKNEDIPIKKVFADINFRIKEEFSENLLT"
     regulatory      44345..44446
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            44667..46202
                     /gene="guaA"
                     /locus_tag="PC414_001119"
     CDS             44667..46202
                     /gene="guaA"
                     /locus_tag="PC414_001119"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDF2463526.1"
                     /translation="MKHELVLVIDFGGQYNQLIARRVRENNVYCEILPCTASIERIKE
                     KNPKGIIFTGGPNSAYLEDSPTISKEIFELGVPILGICYGIQIMSHVLGGVVRKGNKQ
                     EKEYGKTAITYGKSSLFEGITTNSVWMSHTDLIEKVPEGFTIVANTNDCPVAAMENVE
                     RNLYGVQFHPEVEHCLEGDKILTNFLYNICKVKGDWTTDSFIEDKIKELKEKIGDKKA
                     LCALSGGVDSSVAAVLIHKAIGDNLTCIFVDHGLLRKNEGNDVERIFREKFDINLIRV
                     NAEDRFLSKLKGVSEPEAKRKIIGEEFIRVFEEESNKLGKMDFLVQGTIYPDVIESGH
                     GNAATIKSHHNVGGIPEDVDFQEIVEPLRELFKDEVRKIGLELGIEEGLIFRHPFPGP
                     GLGIRVIGDVTKEKCDILREADAVYMDELRKAGLYREIWQAFATLPDVKTVGVMGDER
                     TYAYLVGLRAVTSSDGMTSDWYKMPYDVLERISNRIINEVDGVNRVVYDITSKPPGTI
                     EWE"
     gene            complement(46279..47325)
                     /locus_tag="PC414_001120"
     CDS             complement(46279..47325)
                     /locus_tag="PC414_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDF2463527.1"
                     /translation="MPAYKDEQRKSWYASFYFTDFDGDRKKKIKRGFKTKKEALEFER
                     EFLNKSKMSTDMSFESLIEEYMHDMSSRLKLSTLETKKYLINFRILPFFKSLKINEIT
                     ATHIRRWQNELLKSDYSQTYIKTINNQLVAVLNYAVKYYNLPSNPAHLAGSIGKKNAD
                     EMNFWTLEEFKKFIEFEKKSEPRLAFEILFWTGLRLGELLALTPKDIFENKISVEKSY
                     IRLNGEDIVSSPKTPKSKRVVPIPHFLYNNIKDYLSKLYDLKDNERIFKFAKSYLSKE
                     LDRCCKLSNVKRIRVHDLRHSHASLLVNMDVNILTIAERLGHEKVETTWNTYSHLYPN
                     KQLEVAQKLDNLNI"
     gene            complement(47402..47617)
                     /locus_tag="PC414_001121"
     CDS             complement(47402..47617)
                     /locus_tag="PC414_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2463528.1"
                     /translation="MLKKLRKKKRMTQLELAEKMRRNRSYISKLENQEYKDIGISTIL
                     DLSIALEEDFLELCKYYKLQEIKRRGK"
BASE COUNT        18364 a         5509 c         7905 g        16212 t
ORIGIN      
        1 aaaaataatt ttaaaatatt taaatcattg atttcaaggt attgtatttt taaaagagta
       61 aaaatataat aagttaggta tataatatat ataaagtaaa aaataaaaaa atggaagtga
      121 ttgagatgag tagtatattg ttggttgaag atgatttaag tttgatatat ggattagaat
      181 attctataca aaagaatgga tttagtgttg atgtagcaag aacagtagaa gaagcattgc
      241 aaatttataa agaaaaaaat tatgatttac tgcttttgga tgtatcctta cctgatggtg
      301 atggttttga tatttgtaaa agggtaagag aagcttctaa tgtaccaatc atatttttaa
      361 ctgcttctga tgaagaagta aatgtagtga tgggacttga tatgggtgga gatgattaca
      421 taacaaaacc ttttaaatta aatgagttaa tttcaagaat aaaagcttta cttagacgtt
      481 ataattttac aagtgaaaat gtaactgaac taaaatcaaa taatattact gttaaattac
      541 ttgaaaatcg tgtttttaaa aatgaaattg agcttgaact tactacagca gaatataaat
      601 tattatgctt acttatgaaa aataaaaata ttgtacttac aagaaaaaat atattggata
      661 agttatggga tggaaatggt agttttgtag atgataatac tctgtcagta tatgtaagaa
      721 ggttaagaaa taagattgaa gataatcctg aaaatccaaa aaatctatta acagttagaa
      781 gaatgggtta caaatggaat gttataaagt gagggtgtta atatgaaggt attttctaat
      841 aaagacataa aaatattttt tttggtgatt atatccgttt tattgatgtt tatagtagta
      901 ggtcaaatag tagttataaa tataactaat gattataaaa gtgcactgtt agaacatgat
      961 tataatatag ctggatactt aaatagtatg ggtgttgata aatcaaaaat accagcagta
     1021 tttactacta atgaagataa atatacttta aaaggaaaag aaatcttaaa tacagctaga
     1081 tatgactttg gtacagatga tagttttatg ccaagtgtga aattgtttca aggtaaatat
     1141 ataaaaacta tgtttttgta tttatttgtg ttctttacta tgattatttt tatattgtat
     1201 gtgtattttt ttaggcaaca gaacaagata gaagaagcta gttataaaat tgattcattt
     1261 atgaatggaa atataaatgt acggttaaat acttatgaag aaggtagttt atcaaagtta
     1321 tttggttcaa tagatgctat gtcaacatct ctaaacacac atatattgaa agaaaagcag
     1381 aataaagaat ttcttaaaaa tacaatttca gatatatcac atcaactaaa aactccactt
     1441 acagcattga tgatgtataa tcaaatattg caggaagaga gtcataatag tgaaattgtt
     1501 gagaattttg tgcagaagag tcaaaatgaa ttagagcgta tagaatcatt gatacaaaac
     1561 ttgttaaaga ttacaaagat agattcaaag acaataattc ttaacaaagc tagtgttaac
     1621 gtaaaaaagt tgattactaa aattttatat agttttgaaa ctagagcaga aaaagaagga
     1681 aagagtatct ctataagagg tctagaaaat acaaacttgt tttgcgatta tgaatggata
     1741 tcagaagctt taagcaacct tataaaaaat gcattagata atacaaaaga gaacgataaa
     1801 ataatgatag aatgggttga aacacctata acaactatta tttcagtgag tgataccgga
     1861 aatggaattc atattgaaga tatacatcat atatttaaaa gattttatag gagtaagttt
     1921 tcaaaaagta atcaaggcct tggattaggg ttgccactag ttaaatctat catagaacag
     1981 cacaatggaa ccataactgt agaaagtaat tttttacaag gaagtacatt tactctaagt
     2041 tttttaaagc ttactaatat gtaagatagg atacatctta cagtaaggtg tatgaattat
     2101 tcttatatta atcatcagaa aggagataga gcaaatggat attcttgtgg ttaataattt
     2161 atgtaaaaca tatggaaatg gatatacaaa agtagaggca ttaaaagatg tatcatttac
     2221 agtttcaaag ggagagtttg ttactgtagt tggagaatct ggctcaggta agagtacatt
     2281 attaaatctt ttaggaggtc ttgatactcc aacctcagga aaaatatttg ttgatggtaa
     2341 agaaatatgt tcaatgaaag aagaaaaaca gactattttt cgacgtcgta atataggttt
     2401 tatctttcaa tcatataatc ttatacccga attaactgtg gaacaaaata taatatttcc
     2461 tttactacta gattacaaaa aaccagacac tatttatcta gatgaagtat tgacaatttt
     2521 aggtcttaaa gatagaaaaa gtcatcttcc aaatcaatta tctggtggtc agcaacaaag
     2581 ggttgcaatt ggaagagcat tgattacaaa gccatcaatt atattggcag atgagccaac
     2641 aggaaatctt gacagtaaaa atagcagtga tgtaatttca cttttaaaag tgacagcaga
     2701 aaaatataac caaactatta ttatgataac tcataataca aatatagctt ctacagcaga
     2761 taggatacta caggtttctg atggaatgct taaagaccta ggggtgaata gagaatgaga
     2821 agctatttag aactagtacc acaatatgca aaggtacata agaaaaaaaa tagaactaca
     2881 attttatgta ttgtcattgc agtatgttta gttactgcta tttttggact agcagacatg
     2941 gctatacaat gtcaaaaagt acaatttata aaagatgatg gaaattggca catagtactt
     3001 aaaaatctta atgaagaagc tatatcagaa ataagtagta gagtagatat agctcactat
     3061 ggtagaggac aagtcattga taatggagag tttaaatcaa aggagatttt gatacaaggt
     3121 ttagatgaag atttagcaaa acaattaggt ctagaaataa taaaaggtcg ttatcctgtt
     3181 gtggagaatg agactttaat agatgtagat gcagttaaaa gctttaatct aaaagtagga
     3241 gattttatca aaataaaatt agcagatggt agaaataaaa attataagat agtaggtgaa
     3301 tattcatcat tatcaagatt aagagcctca gataagcatg gattaatgct ttcaatatta
     3361 ggtacacaag ctatcaaaaa atcagaaaca tatgattatt attatattac atttaaagac
     3421 aaggttaata ttagaaaagc tacttctgat attaagaata ccttcaattt aagtgataaa
     3481 caaatcggta aaaatgaaaa attactttct attataggtc aaggtgactc tgattattct
     3541 aaaccacttt acttaacagc aggaggactt tttatacttg tgttaatagc aagtattatc
     3601 atgatatata atagctttaa tatgggtgta atagagagaa taaagttttt tggactatta
     3661 cgttgcttgg gagcatcaaa actccaagtc aaaaaatttg ttgttttaga aagtttaatt
     3721 ctaagtgtaa aggctattcc actaggattg ttattaggat gtattgtaac cattatatcc
     3781 tctatatttc tcaaatatgt gaatacagag ttatttggtt cgatgccaat tttaaaggtt
     3841 agctttattg gtattgtgtt tggaataata gtaggatttt taacagtaat attatcagct
     3901 attgttccag caaaaaaagc ttctggggtg tctccactaa gtgctatacg tggtaatcta
     3961 atgttcaaag aaattttaac tagtggaaaa gcaagtaaaa ttgctaacgc aggaagttct
     4021 aaaattgatg tttctatggg aataaatcat gccttttcaa gaaaaaaatc ttttatactt
     4081 atgacttctt catttgctat tagtattgta ttgtttttag gttttagtgt atttatagac
     4141 tttatgtatc aggcacttaa acctataaaa ccagaagctg ctcatatatc tattgttgac
     4201 gctgacttta agtctgttct tactaaagac gatattaatg aaataagtaa tatcaaagga
     4261 ataaaaaaag tatacagtag aatgatgacg gatgtagagg ctacatacaa taataaggag
     4321 gcaacatctc agctaatttc ttatgaaaaa aatcaatttg actgggccaa aaagtatcta
     4381 gtaaaagggg aaatcaatga agataaatta gagtcagaaa atatcggatt ggtagaagaa
     4441 ggtcatgggt ttaatattgg agataagata tatattaaaa atggaaattc taagaaagag
     4501 atagaggtag caggtatact ttcttcaatt tcatttgatg ttaaagacaa atatattgga
     4561 aatattataa catctgaaaa tacttttact agtataacag atataaaaga ctatataata
     4621 attgatactc aagttgatga agatgctcct gaagatttaa tccaaaatat aagaaatgtt
     4681 ctagatgata atttagaggt tcgagataag cgacaaggta acttagaggc taaaaattca
     4741 ttttacacta tggcagtttt tgtatatggg tttgtattta taattgcgat tataagtatg
     4801 tttaatatca taaacagtat gaatataagc gttacaagta gaataaatta ctatggtatt
     4861 atgcgtgcaa taggcatgtc caataagcaa ttaagaaaaa tggtaatagt agaatcaagt
     4921 acatatgctg taagtggctg tttattgggg agtgtacttg gattaatatt acatagatat
     4981 atatttgtat ccttagtaac attaaaattc catatagact ggcaaatacc ttttgattta
     5041 ctgtttatca ttgtagtaac tatgataata ataactttgc ttgcagtaag aaaaccaatt
     5101 aaaaagattt gtgaattaga cataatagag gtagtaaatg cacaataaat ataagtagta
     5161 atttaaatag aggtagtaat ttaaataaaa aatcgaagta aatatgaaat agaaaatatc
     5221 tttcataatt tacttcgatt tttgtgttat ataatttact ttagataagt tccatcttca
     5281 tcttgataaa ttaagtcttt cttcataagt actccaagag cacgtttaaa attttcttac
     5341 ttgaattaaa taagatagaa atatctttag gatcagattt atcattgaat ctcatatatc
     5401 catctgaacc ttctaaatag cttaaaatct tattttctaa ggtatcaagt tcatcttttc
     5461 tgtttcctct aggagaaagt cctaatttac catcatcgta tatttttata acatgtaaat
     5521 tttcaagaac atccccagct tttaattctg taaagtattc attatgaagg attacaccag
     5581 catatttatt gtcaacacat atcatagcag agttgttggt ttggaatcca tatacaactc
     5641 catttacaac atctcctaca ttgtaagtat ggtctgtttc taaatatgaa tctatgtctg
     5701 tagtagcagc tattcttccg cttttatcca agtatatata gaataaatat ttttcatctc
     5761 taaacaatgg ataagtctta gctttaaaag gaactagtat gtcttttgta agtcccatat
     5821 ctataaaagt accaatatcc gtgttatcaa ctacttttag atacgtaaca tctccatttc
     5881 taattatttc tatagtatta gaaaaataat ttttaaatag tttgtaaaat attattgaat
     5941 tttttctctc gaaatatata atttagaatc ttctgattta tttttatgct cgatttcctt
     6001 attttttttc ataaatatat atagaggagc tattaacaac attgccccta atcctattaa
     6061 aactctattt tgagttatag gttttgaaat atcttctgcc caagcttgtt gtaacaacca
     6121 taagctacct aaaatagcta ccattggtat aacaggacca aatggaatct taaaagaggc
     6181 cttaacatct gttctttttc ttaaaactat tactgataaa caagttggta tgtactgagc
     6241 aaatcttgca attacactca ttactgcaag ttgttcaaaa cttccagtta gcacaagtgg
     6301 tatacaacaa attaaagata ttattattgc tacatatgga gcaccatatt tatttgtttt
     6361 tcctataaat gcaggaagag aaccttcttc tgctagagaa gaaccacatt taggagttac
     6421 tattgatgaa cctatattta taccaaatat agatattaat gttgctacag atatgaaaaa
     6481 tttaccataa ttgcctaaaa atacacttga agcatctgca attggaatac tgttttcaaa
     6541 tagtctatct ccaagtatac ccatacagac aatttgaatt aatatataaa ttattgcaca
     6601 tataaatatt gtggttatta atgcgactgg aagatttttt ttaggatttt ccatttctcc
     6661 acttgccaca ataaatgatt caaagccggt aaatgcgtaa aatactaata taatggctgg
     6721 acccatacta gagttaacta catttcctga aggaacaata tttacaaatt ttataaagaa
     6781 tattccaact attataaaaa ctattaatgg tactaattta gctatagtta ttacattatt
     6841 cattatcttt gtgcttttaa ttccaaataa gctatttatt gaaagtagtg ttacgagtat
     6901 tgcagctata taacctttgt attcagtggc agattctggc caaaatgaac ctaaagctgt
     6961 tgcaaatcct actgctagag tagaccatga tataattcta ataacccaag acattgtacc
     7021 aacttcaaat cctataaaat ctccaaaagc ttttttggag tatagatatg ctcctccatt
     7081 cttatcaaac atgcttccaa cttctgcaaa acataaaagt attgatagaa ctaagagtgt
     7141 agcaaaaatg tatataaaca tactgttttg acctgctaga ttataaactt tacctggtag
     7201 taagaatata cctgaaccta ttatgctatt aatccctaat aatatcatac taaatagacc
     7261 taatttctta gctttttcca tattaattta tctcctattc ttttattaat tactgtacta
     7321 attaaaatac aaagtattca ttacatgcat cataacacct attggtaaac aatcttcatc
     7381 tatattaaag ctagaggtat gaagaggtga aattaagccc ttttcctcat ttttacatcc
     7441 taaatggaag aatgctcctt tacaatgttc tgtgtaaaaa gaaaagtctt caccacctaa
     7501 agacgggttt ggtctcaata taaatttttc ctctccaagt aattctttag tatttgatat
     7561 aactgtatct actaattctt tttcatttat aacagcagga tagttttcat cacttacatg
     7621 taaagtccca acacaaccaa atgcagatgc agtgtctcca acaattttag ctattttatc
     7681 aatcataaag tttcttgttt tgctatttaa ggttcttaaa gtgccttcta gttttacatc
     7741 ttcacatatt acattgtgtg catcaccacc atatatttta cctatagtca aaacagcagc
     7801 attatttggt tctaagtttc tacttattat agtttgtaat gatgtaacaa tttgtgaagc
     7861 tgtaactatt gcatcaatac cattttcagg atatgcacca tgagctcttt tacctttgat
     7921 acttattttt atagtgtcta cactagcatt taatgtatca tattttattt cgataaatcc
     7981 tgtttcgata tgtggcataa catgaagtcc gaatatataa tcgaccttag gattttctaa
     8041 acatccatct tcaactaaga attttgctcc tccaaaacct tcttctgctg gttgaaataa
     8101 gaattttaca tttacattaa gtttatcttt tattgaatgt aaaactttac atgctccaag
     8161 tagcatggtt gtatgtgcat catggccaca agcatgcatt tttccagaat gtattgattt
     8221 ataaggtatg ttgttttctt cttcaatagg aagagcatcc atatctgctc ttatacaaat
     8281 cgttttatct gcactttctt ttaaaatata tgccataatt ccattgtgtt ttgtaaattc
     8341 catataatca attccgattt cttttaaata agaaattacc ttctgtttag taagattttc
     8401 ttcaagagct aattctggaa ttttatgtaa ttctcttctt atgtttataa gccatggttt
     8461 tatagaatta caactttcta ttacaaaatc ttttcctaat tctttggttt gatattttaa
     8521 tttcaaagcc tgttgcattt ttattcctcc aattgctttt tattgtttta ttaatataat
     8581 aagcaattat tatgccataa ttttcgatgg agaactttgt tttttataag aatattgaat
     8641 aaatataatc atttatctat atgtatagta taaaaaatga ttgcatagtt tcaattttca
     8701 gtaaaaaata ttattttgaa gaaaatatat tttatatgct agtttatata aaaaaaatag
     8761 gaaataaaaa aattatccta ttcctattaa ttcctattat ttatgtacaa aataaattat
     8821 aatttcaggg tacttgaaaa ataaaaaaac ctattgaagg agtactttaa tcctgaatag
     8881 atttctttgt ttgataaatt ataattttat agacctttat attttaattt attgtaaaaa
     8941 tcttctcctt tttgagttat tttacttcca tatcttcctg aactacaaat tataagttct
     9001 tcttttttta gatattcaaa tattttttta atttttcctt ctgtaatttg aaggttctta
     9061 tcgagtagtg cttttaaaat taatcctcta ccaataccaa catctgattc cttatttaaa
     9121 attagtatct ctaatatatt caatatatca ttcttactat attttagttt taatgatgta
     9181 tttttgttat ctgaagacct taagtttgga ggtaaatctt tctctaatac aatatcttca
     9241 cacataatgt ttaaatagga tgtaacattt tggagttctc ttatattacc tttccacata
     9301 tagttttgta taagtttttt tgcttctggt gtgattacta atttcttatt aattaaatct
     9361 tccataataa ttaatatgtc ttcttttctt tctcttagag gaggtatatt tattggaatt
     9421 gtatttaatc gataatataa atcctctcta aattggctat tatcaatcat ttgttctaaa
     9481 ttcttatttg tagcagatat gattctaaca tctatattaa ttacattgtg agacccaaca
     9541 ggcattatct gcttttcttg aagaactcta agtagttttg tttgtaattc taaaggcata
     9601 tcgcctattt catctaagaa tattgttcca ttattagcaa gttcaaataa tccttgtttt
     9661 ccatctttta gacctccagt aaaagttccc ttgtcatatc cgaatagctg gctttctaat
     9721 aaattttcag gaacggctgc acaatttact gctataaagg gttgattttt tctatttgag
     9781 ttgttatgta tagactgagc tatgagttct ttaccagttc cactttcacc tctgataagt
     9841 atgcttaagt cagatttaga aaccttctta gctaaatcta tacattcaaa cattttagga
     9901 ctatttgttt ttatatcctt aaatgtatat tttgctattt gtcctttttc tcttaatttt
     9961 ttagtgagat tttgttctaa tttttttatg tatgttattt cttgtaaact ataatatgtg
    10021 cccatttttt catctctatt gtatatgttt tttttattta catttatata tttcttattg
    10081 aatacgacaa ctttatctag tatctctttc tcactcaata aaacttttaa actatctaca
    10141 aatatatcct caatgtattt tccatatata tcctcattta tgtctaaaat atcttttact
    10201 ttactattat atgtatttat ttcaccatct tttgaagtaa ataatatacc atcttttgat
    10261 aaattcaata ttgtatctaa ttcttctatt tttaaaaata attccttata tttatctttt
    10321 ataccagtat ctaaacttat tatttcttct gagtatttaa ctagattaga ttgtatttcc
    10381 tttgaatcaa tttgtaataa atttattatt tctataaatg tagaaatatc tatatatctg
    10441 tgacctaaat caaatatatc tgagataaag ctaggtactt tttctggaac tcctggagtt
    10501 atagctattt ttatattttt atagttatta tcattcatat atggaattgg tctaatatta
    10561 gtcactccaa ttttataaaa taaacttata gtttctagag tagtttcatc agaatcattt
    10621 acgattagaa catttgtacc ctgtggtaaa gatagtagat tataaatttt atcttcccta
    10681 aaagttcttc ttaccacaat cacttttttc ttatctagta tattatttat aattttgtct
    10741 aatctttcta ctgacattac taatatcaca tcatcattta taatatcatt atcatttatt
    10801 tcatttatga agtatctatt aatatttata gaatctccaa aaactaattt caaattattt
    10861 tctaaaaagt ctattaaatc ttttctacta tcatttacca gagctactga ttttttcatt
    10921 tatttctcct tatataataa atagtttcca acaaaatagt tgtataaagc aatataattt
    10981 tgataattta aatttatcaa ataaaaaaga aagtgtctat aatgacttta taaaagttgc
    11041 attaagacac tttctttctt ataattaaaa atcttgtcac caaatccccc taattgttgc
    11101 ttaaaactta aactgtatgt atatttttca tttctattcg attaaattat aatatacacc
    11161 caaaaaaatt tattttaata aattctattg ataatataat tttgtaaata tagaattata
    11221 ttaaaataaa ttatatatta aaatatatat gtataagttt attctgcgtc agtagaatga
    11281 atttgtcaag tttaatttag ttgtaatttt atcttttatt aaaaataata taaattaaaa
    11341 catataaata aaatatttta tacaatatat tgtaaaggag gtaactttga tgaaagcaga
    11401 aaaaacaagt atatatccaa ttattaaaaa taatttaaac aaaatatcaa ttgttgaata
    11461 taaaaagggt caaagatttg tagcatcaga taaagactta caagaagtgt tctttataat
    11521 agagggagtt gcattagtgg agtgtactac tagaggagga aataaatttc ttgttgacat
    11581 tgtacctgaa aatgaattta taggcaaaat tagttacata tatgaacata atttgaaatg
    11641 tgatatattt gcaaagacca atataaaatt atttcgattt gaaaaaaatg tatttgaaga
    11701 gttttatctc aaaccagatt ttattgcact atttcacaga aaatgtacaa gaagaatata
    11761 tgaattatat aaaaccagaa tggtaagaga actattttct tgcacagaag ttattgccta
    11821 ttgtatatca aataaacaag aaaataatgt ctgcaatata aaagctattc gtaatttcag
    11881 tgaaatctat tcaattagta gaaaaagtag ataccattct ctgctaaagt taactgaaaa
    11941 ggagatagta aaaaaaatgg gtaatacata tgaaatttta aactatgatg aattacataa
    12001 attggcattt gaagtaaaag aatttttgga agatgagtaa aaattgttac ttaatcaaaa
    12061 taaagtataa taatttctgt aaaaaataga aatagtctca ctgaaaatca aaaatacttt
    12121 agttattttc aatgagactg tttagaaata ttgtatactt ataatgtata gtataattat
    12181 taatcaattt cctttactat attaatagcc cattttttag ataatgctct aggaacacca
    12241 aatataaact taaccacttc ttcactataa gtgagcaaaa cgagtacaaa tataggtgca
    12301 cctttaaatg cagctataaa agttagagga atagaagcta accacataca tcctaattct
    12361 aaaaatagag catatttagt atctcctcca cttcttaaaa taccaattat aaccaatgta
    12421 ttaaaagatt taagagccat tagtattccc attataaaga atatcttagt tatatcagaa
    12481 gctaagctat cagaaacact aaacattttt aataataatg gtatatttaa aattaaaata
    12541 attccaagta ttaaaccagc tgaaaataca agtatagaaa actttttagc atattcaatg
    12601 gctcttttaa tatggtcagc tccaagttca tttccaagca taatagatgc accagatgca
    12661 atacatacag ctgtcattat gaagaaatta cctgtactgg ttgctatctg gctagctgct
    12721 atagcagaag taccagctct tgcatatgca acagaataca ggacagttcc aattgcccat
    12781 acagagtcat ttacaaatat aggaaagctt tttgatgaaa aagattttat aaaaatttta
    12841 tcaatagctt ttagattttt tagaccaaac tttaatatat agtctttttt ataaaggtaa
    12901 acataagtaa tcattaaaat taattcacat attctagcaa taacagtagc tagagcagca
    12961 ccttttacac ccaatgcagg aagaccgaag ttaccaaata taaatccata atttaaaata
    13021 acatttgtta caagtgcaaa tgcactacaa atcataccta acttaggatt tctaacacct
    13081 cttgaaccag tgctaaatac agtacttaca gcaattaatg gataactaaa tactgcaata
    13141 ctaaagtaat ctatacaaag ttttaccacc tcagaatcat acgaaaatat gtgtataatt
    13201 ggctttggtg ttagtaaagc aggaataaca aatacaaaac ttaaaacaat tgccaataca
    13261 caagtaagac ctgtgacttt tcttatatta ctcacatcct tctttccata aaactgtgaa
    13321 ataaaaactc cagcacctcc tgtgatacca gataaggaca tattaaaaag aaagaaaaat
    13381 tggtttgcaa ctccaactga agctacagat gtttccccca aactacttat cataattgta
    13441 tctattacat ttacagaagt agaaattaag ttctgtatga taataggtat acataaagat
    13501 agaagtattt tataaaatct tttattttcg tcttttaaag aaaatagttc ttgcaaataa
    13561 tcctctcctt tttacataaa ttcttcccac tcatcatata gagaggcaag gttattttct
    13621 aaattaattt tttcttggct aacttcttta gctttatctg gattagagta tacttcttct
    13681 tgacataaaa gtatatctaa tttttcaatt tcaatctcta acttttcaat ttctttttct
    13741 atattttgtc tttttattct atttttcttt tctatttctc tttgttctct ctcttttctt
    13801 ttttcatctt ttatttgagt tctagttttt tcttcttttt cttctataat actcatctct
    13861 tggatttgcc tttttttatc tatataataa tcataatttc ccagatattc agtaattcca
    13921 ttttcatcta aaactaaaat tttatcaaca acagtattta gaaagtatct atcatgtgat
    13981 attgtaaata tagtcccatc ataattagtt aatgcctctt caaggacttc cttagaatca
    14041 atatctaggt gattagtagg ctcatctaat aacaataagt ttgcatttga tagtataagt
    14101 ttaagtatgg caactctagc tctttctcca ccactcaatg tagaaatctt cttaaaaact
    14161 tcttcatcta caaataaaaa tgcccctaac atatttctca aagtagtttg agtaagatga
    14221 gtattatttt cccaaatctc atcaattatt gtgttatcta aattaagagt tttttgttcc
    14281 tgatggaagt aggaaacatg tacattagtt ccaaatttaa tattgccact aagaggttgt
    14341 agttcattag ttattatttt aaataatgta gatttgccaa caccatttgc acctataagt
    14401 gcaacttttt cccctctata tatatctaaa tccaagtctt taaaaagtat tctttctcca
    14461 taacccatag atacatctct aatttgaaga acatcattac cactctggac agaaggcgta
    14521 aattgtatac gagctttttt cctataagct tctggtttat ctaaaacgtc taccttgtct
    14581 aatgcttttt ctttacttct tgcacgcttt aggtgttttt ctctaccata agcttttagt
    14641 ctttcaattg attcttcttg cttttttatt tctttttgtt ggtcttcaaa tttcttaagc
    14701 tctagttctt tttctatttt agataactca acaaatttag agtagttacc attatatact
    14761 ttaagttttt tgttgtgaac ttcaaaaatt ctatttacgg attggtctaa gaaatatcta
    14821 tcatgagata ttaacatgac agtcccttta tattgtttta aaaatacttc taaccattct
    14881 attgcttcag agtctaagtg gttagtaggt tcatcaagta acaatagagt tggtttctta
    14941 agaagtaatt ttcctaaaag aactctagtt ttttcaccac cagaaagtat gcttattggc
    15001 ttatccatat cattgtcact aaatcctaat ccctttagaa ctcctttggc ttcagattta
    15061 tatccatatc catttaaatc tgaaaataat tctagtttat gtgaatattc atccatgatt
    15121 ttttctataa ttggagagtt tgtttttgta ctttcttcag atattttaac ctccaattct
    15181 cttaaatagg attccatttc tatcaaattt ttaaaaactt ctagtacttc ttccaaaata
    15241 gtattatccg aataaaaatt ggtattttgt tctaaatatc cgatttcaca atctttagaa
    15301 gtgtaaatat ctccactatc atatccataa attcctgaaa ttattttaaa taaagtagtt
    15361 tttccagtac cattaacacc gattatacca attttatcgc cttcatttac tgtaaaacta
    15421 atattttcta atatagaatc tataccaaaa cttttattta agttattaca tgacaaaaca
    15481 atcataaatt tctacctcct tgaactatct aaaattataa tgttttgata taatataatc
    15541 aaggttatat gtattagttt tgaaaaagaa atattttatt gtgaatcaaa caaaaaagag
    15601 gacactttta ggaggtgaag atgttgggaa ataaaaatat atcaatggca gttataagaa
    15661 ggctcccaaa atatcataga tatcttggag acttattaga tagggatata caaagaatat
    15721 cttctaaaga attgagtgat ataatagggt ttaccgcttc tcaaataaga caagatttaa
    15781 acaactttgg tggatttgga caacaaggat atggttataa tgtagaagct cttcatactg
    15841 agataggtaa aattcttggg ttggatcgac catacaacgc agttcttgta ggagcaggta
    15901 acttaggaca agctatagcc aattatgcag gatttagaaa agctggattc gagataaaag
    15961 ctttatttga tgcaaatcct agaatgatag gtttaaagat aagagagttt gaagtattag
    16021 attcagatac tttagaagac tttataaaaa acaataatat agatattgct gtattatgta
    16081 tacctaaaaa tggagcacaa gaagttatta atagagttgt aaaagctgga atcaaaggtg
    16141 tatggaattt tgcaccttta gatttagaag ttccgaaagg tgttatagtt gaaaatgtaa
    16201 acttaacaga aagtttattt accttatcgt atttaatgaa agaaggaaag tagacaactt
    16261 aaacatatta ataatgagca gttgtgaaga gtattgagaa agtcagaaga tttaatatta
    16321 aatcctctgg ctttttgaaa tattaggata aaaatttaaa taatgtatgg aaataaacaa
    16381 tattaaggag agtggttttt gtggaggaat tatggaaaaa ggcaattgaa tttcatggtc
    16441 atgaatgtcc agggttagct ataggattta aagcagcact tgcagcaaga gaatatttag
    16501 gatgtaaaca gtcaggagat gaggaaattg tatgtatttc tgaaaatgat gcttgtggaa
    16561 tagatggtat acaagtcgtg ctaagttgca caatgggtaa aggaaattta ctacttagga
    16621 tgactgggaa atcagtatat catttcttta atagggaaaa tggaaaatct ataagaatat
    16681 atgtaaagtc aaagccattt caaatggata gagaaggata caaaaagtac ttattagaaa
    16741 gtgattttga agaaatattt gagattacag atactaagtt aagacttcct gaaaaagcaa
    16801 gaatatttaa atctcttaaa tgtgaagtgt gtggtgaatt tgcatctgag caatatataa
    16861 ggattgaaga aggaaagaaa gtatgtcttg attgctttaa taaatatgaa agattttatg
    16921 aggagtatta aaattataaa ttagattaaa gttaaaagta ttagatatgt gctaacagta
    16981 ttttaagatg aattaggtag caacataata aagtatataa ataatattaa ttgaatcata
    17041 cataattaaa aagaggctga ttagtattta taaaataaaa tcagtctctt tatgaataac
    17101 gaattattat tctggttgag gtgcatctac aggacaagct cctgcacaag aaccacagtc
    17161 tatacaagaa ccagcatcta taacatatgc gtcgtctcca gctgatatac agctaacagg
    17221 gcattcagcc tcacaagcac cacagcttat acaagcgtca gtaattttat atgccatttt
    17281 gaaatcctcc cttttatatg tattgtaatt caataacatt ataacaaata ttattgaata
    17341 taacaattaa attaattcta caacaaaaac tttattatga tattcttgtt ttaaagtttt
    17401 acaataaagt ttaatctgct tatattttta tttattttaa agaagtttat tttaaaaata
    17461 aaattaaatt taaaaagtta tgtattgatt atatttaaaa atgttatact ctaaaaaagt
    17521 atattttatt taaaaattta ttaatacaaa ggagatttac tatgaaaaat aaattagttt
    17581 acatatgtat tatgagtcta ttgctatttg tagtaggatg ttctaataat ccaaataagg
    17641 aaagtaccat taaaaaagac tctaagaata accaaagcca agttaaagaa gaaaaaagag
    17701 aacctgtgaa aaaagatata actataagtt tcgctggtga tgtaactatg gggaactata
    17761 aaggttcatc atattatggc agttttgacc atgaatttaa aagacaagga gaagattaca
    17821 attattttct taaaaatgta aaacctgtat ttgaaaaaga tgacttaaca gtggttaatt
    17881 tagaaggtcc acttactaca gcgtcaaatg caaaagaaaa aaaatttgca tttaaaggaa
    17941 agcctggata tgtaaatatt ttaaaatctg gaagtgtaga ggcagtaagt attgcaaata
    18001 atcattcaga agattatttt gaagaaggaa tgaaagatac acagtttata ttggacgaga
    18061 ataaaataaa ttattttgga ttagggaaag atgctgttgt agatgtcaaa ggaattaaag
    18121 tgggtctatt agggtataat ggattatcaa ctgaatataa tgaaaaaaat ttaaaacaaa
    18181 tggaagatga tattaagtcc ttaaaaaaga aatcggatgt tgttgtagtg tactttcatt
    18241 gggggataga attagattat tatccagata aaaaacaaaa agattttgct cattatgcta
    18301 tagacaaggg agcagattta gttatggggt ctcacccaca tgtgattcaa ggaatagaaa
    18361 agtataaaaa taagtatata gcttatagtc tagggaattt ttgctttgga ggaaataaga
    18421 atccttctga tacagatagt tatatatatc aacaaacctt tactttttca gataataaat
    18481 taacttcaat taaagagcca aatataatac caacttcaat aacatctagt aactccagaa
    18541 ataattatca accaaaaata ttggatggaa gtgagaagga tagagtttta aataaacttg
    18601 aaaaaataag caaagattta aataaataaa atgtaaaaaa aaataaatat atatatttat
    18661 tttaataatt tatattttgt ttagggtatg gtaaatcgtg gtattaaata agtagatttt
    18721 aatagttaaa ttatagtcaa ttataataaa gctatttaat ctactttttt aatataaaaa
    18781 tataaggggg aatttgtaat ggcttgtaac acttgtaaaa tgtgcgaaag tgcagacaag
    18841 aaattagaaa gttttgtagc ttcaaaggat gttgaaacag catttcatag aacagaagat
    18901 cagaaagtta agtgtggttt tggacttcag ggggtttgtt gtagactctg ttcaaatgga
    18961 ccatgtagag taactcctaa atcaccaaga ggtatatgtg gagcagatgc agacactata
    19021 gtagcaagaa actttttaag agcagtagca tcaggagcag cttgttattt acatgtggta
    19081 gaaaatacag ctaagaattt aaaaaatgtt ggaataacta aaggtgttgt taaaggagaa
    19141 aaaacattaa atcaattagc agagatgttt ggaatagaaa gtgatgaaaa gtatgataaa
    19201 tgtataaagg ttgcagacaa agttatcaac gatttgtata aatcaagaga tgataaaatg
    19261 gaattagtag aaaaaatagc ttatgcacct agagtgaaaa agtggaaaga gttaggtata
    19321 atgcctggtg gtgcaaagtc agaagttttt gatgcaatag taaaatcatc aacaaattta
    19381 aatagtgacc cagtagacat gctagttaat tgtttgaact taggtatatc aacaggatta
    19441 tatggtctta cactaactaa tcttttaaat gatgttatgt tgggagaacc agttataaga
    19501 atggctcctg taggatttaa tgtaatagac ccagactaca taaatataat gataacaggc
    19561 catcaacatt caacatttgc aaatttccaa gatagattaa aagatgaaga tgttataaaa
    19621 ttagctcaat ctgttggtgc aaaaggcttt aagctagtag gatgtacatg tgttggtcaa
    19681 gatttacaat taagaggaga acattatcaa gaagtatttg ctggacatgc aggtaacaat
    19741 tttacaagtg aggctgtttt atcaactgga gcaatagata tagttctttc agaatttaac
    19801 tgtactatac cagggcttga accaatagca gataaatata aagtaaaaat ggtttgttta
    19861 gatgatgttg ctaaaaaagc gaatgcagaa tacataggtt tagacagaag taaattagat
    19921 gaacttagta acactttaat agaaaaagca ttggagtctt ataaggagag aagaggaagt
    19981 attgaaatag atataccaaa agaccatgga tttgaacaat ctttaactgg tgtaagtgaa
    20041 aagaacctga aagcgttttt aggagattca tggaaacctc ttattaattt aattgcagaa
    20101 ggaaaaatta aaggtgttgc agctgttgta ggatgttcaa atatgacagc aggaggacat
    20161 gatgtaaata ctgttgaact tactaaagaa ttgattaaaa aagatataat agtactatct
    20221 gcaggatgtt ctactggagg attagaaaat gttggtctta tgtcacctgg agcagaagaa
    20281 ttagcaggag aaaacttaaa agaagtatgt aagactttag gaataccacc agtattaaac
    20341 tttggaccat gtctagctat aggaagacta gaaatagtag caacagaatt agcagctgaa
    20401 cttggaattg acctaccaca attaccatta gtgctttcag ctccacaatg gttggaagaa
    20461 caagcattag ctgatggagc gtttggtctt gcccttggct tacctcttca tttagcttta
    20521 cctccattta taacaggagg aaaattggtg acagaagtat taacagaaaa attaaaagac
    20581 ttaacaggag gacatgttat agtgaatcca gaccctaaat cttcagctaa tcaattagaa
    20641 gaaataataa tagatagaag aaaaaattta gggttaaagg atgtggaatg caatgcatag
    20701 aatttttata aataaagatt tatgtacagg atgtaagagc tgtgtactag catgtatgct
    20761 aaaacacaat aaagattatg acatgtacac tcttgattta gaaaatatag ataatgatag
    20821 tagagggcat atagaattag atagtaaaca taataatcca gttccaattc tttgtagaca
    20881 ctgtgatgaa cctgaatgtg tattagcatg tatgagtggt gcaatgcata aagatagtga
    20941 aagtggaatc gtttcatatg atgaagagaa atgtggttca tgttatatgt gtgtaatgtc
    21001 ttgtccatat ggattattaa agccagatga tagaagcaag cagaacatac ttaaatgtga
    21061 tttatgtaaa gatgaggagt atcctagatg tgtagctaat tgcccaagtg gagcaattga
    21121 attacagaag gaggaaaatg atgaattatg tagtgttagg ggctagtgca gctggtataa
    21181 atgcagtaaa aacattaaga gaattagata aagattcaaa tatagtagta atttctaaag
    21241 atgaaaacgt atattcaaga tgtatgttgc atcacgttat atctgaacac agaattttaa
    21301 aacaaataaa ttttgttgat gaagatttta tggagcaaaa taatgtaaaa tggattgcag
    21361 gtaagactgt taaaggtata gacataaata aaaaggtagt tcagacagaa gacattactg
    21421 taaattatga taaactttta atagctacag gtgcatcttc tgctattcct cctataaaaa
    21481 atttaagaga tggaaatttt gtatattctg tgagaaacat agatgatatt tataaaataa
    21541 aagaaaaagc acaaaattct aaaaatgtag ttataatagg tgctggttta gttggtatag
    21601 atgcattagt gggattattt aaatatgagc agttaaatat ttctgtagct tttatggaaa
    21661 agtacatttt agatagacaa ttggatgaat atacagcatc agtgtatgaa aataagttca
    21721 aagaaaaagg tgtaaaattt tacccaagtg catcaataca agaaattgta ttggataaca
    21781 gtaaaaatgt tacaggtgtt gcattttcaa atggtgaagt tttagatgca gacatggtaa
    21841 ttgttgctac aggggttaaa ccaaatgctg actttttaga tggaacagga atagaatatg
    21901 atagaggtat aataatagat gatatgtgcc aaacaacaca aaaagacata tatgcagcag
    21961 gagatgttgt aggtaaaaat gctatttggc ctctggcagt aaagcaagga attgtagctg
    22021 cctataatat ggttggaaaa gataagaaga tagaagatga atttgctttt aaaaacagta
    22081 tgaatttcat ggatatacca actatttcta taggaatgaa tacacctgta gatgatagct
    22141 ataaagtgct aactagacat ggaattaatg attataaaaa gtttgttttt aaagataatg
    22201 taatctatgg agcagttata caaggagata tttcatatgt aggtgtatta acttatttaa
    22261 taaaaaataa agttgaaata tatgatttag aaaataggat atttgatata tgttatgcag
    22321 acttttttaa tataaaagaa aatggagaat tttgttatag tgtatagtat atacgtggag
    22381 taaggtttat aaatacagta aatcaataaa tagagttatt ctaaaataag aaaaatgaca
    22441 aatcttattt agaaatagct ctattttttg taattaaatt taaattatat tcaatttgta
    22501 aaatattatt ttgaacaatt atacatatac tctctagtta gaggtaaatc attttttata
    22561 cctttagtga aaagtatttg atgtatatct ataataccag tgttgaaagc agcagcacaa
    22621 gaacaaaggt ataaatccca cattcttata aacttatcat caaacatttc tttaatcttt
    22681 ggaagattct cagagaaatt tttttgccag tatagtaagg tttttacata atgtaatctt
    22741 agactttcta cgtctatagt atgatatttg taatcactac aaatatgtat catttctctt
    22801 aggctaggaa taactcctcc tgggaaaata tattttttta tccaagggtc tccataagat
    22861 tcctgcaatc cacttatata gtgaagtaaa aacaatccac catcttttaa aacagcattt
    22921 acgtttttaa tgaaaagttc atagttttct ctaccaacat gttcaagcat cccaacgctt
    22981 acaattctat caaacataag gtctgatttt tttaattctc tatagtccat aagtcttact
    23041 tcaagatagt cttgaaggtt ctcttcttta attcttgcgt taaattcctt aaattgctct
    23101 tcgctaagtg taattccaac gccgtgaatt ttatattttt ttgcagcttc tattagtaaa
    23161 aatccccaac cacaaccaat atcgagaagg ctcatacctt cctctaaatt tagtttagtt
    23221 aatatgtggt gaactttatt gacttgagca tcataaagag tatcattatc atttctaaag
    23281 tatgcacaag aataactaag tgtcttatct aaccataagc tataaaaatc gttgccaatg
    23341 tcatagtggg catgtatttc tttcttttga tgtctcattg aatttgatga gtgcaataga
    23401 tgttttacag ctgaattatc aatattgaat ttgtctattt gttttaaaag tagatttaaa
    23461 gttaagaata aatctccctc aacttcaatg tctctattta tgtaggcttc tccaagtgct
    23521 aaagatgtac tttttaatag ttcagacttg ctaatatcat tatttactat aactttgaac
    23581 tgaggggagc cctctccaat tagaaattct tcctcatctt taagcctaac tagaaatggt
    23641 gtttcaccaa atttctctaa atagtctttt aaaaaatttt ttgtaatatt catattacca
    23701 gctccttcta atgtaaatcc agtaagtttc cctgcttaaa atatattata gtcctttttt
    23761 taaaaaagta aaattttttc aataaatttt ttatttaaag gaaatttatt ttttataaag
    23821 aaatttcatg ttgtagtata ttttttgctt ataaattttg gtactggata taatattaaa
    23881 ctgttaatga tataataaat ttaatctata aagtaaataa aattttgtaa taaatagtta
    23941 aagctatttt ataaggagat atatgatgga agtatatgct ttggttgggt caagtggcac
    24001 agggaagagc tttaaggctt tagagttcgc atatgaaaat gatatagaat atataattga
    24061 tgatgggatt ttaatataca aaaacaaggt tttagctggg atatcggcaa aacaagcaaa
    24121 tactactata gaagcagtta aaagggctat tttttataat ctggagcata gacaagaagt
    24181 tagagaagca attaaaaaag aaaatataag aaaaattctt attattggga catcaaaaaa
    24241 aatggttaat caaattgtag agagattgtc aattggaagg gtttgcaagt ttataaatat
    24301 acaagatata agtactaaat atgaaataga aatagcaaaa caagctagaa aagaaggaaa
    24361 tcatgtaatt cctgttccag cagttgagat aaaaagtatg gcaagtggtc ttagtataaa
    24421 ttctttgaag agacttttta gaaaaggaag taataggaat atggtagttt tagaaaaaac
    24481 aattataaga ccaactttta gttatatagg aaagttttat attagtgctg atgtaattaa
    24541 acagattatt gaatatgaag tatacaactt tggaagcatt gatagaataa ataaaatcaa
    24601 tatagagaat tgcaataatt ttatgaatat ttttgtaagt ataaatatca atgatttaga
    24661 aactataaaa aaaattgaag acatacaaca gacaataaaa aagtctattg agaaaatgac
    24721 tttagttaat gtacaaaaaa tagatattaa cattcataaa attaaaaaaa taaacttcct
    24781 ccaatgttaa aaaataaact gagaaaatga tatactaatt tgcaataaag tgtgctataa
    24841 ttaaactgta aatttaggaa atggtttttt ctaaatggag tatacataat tataacgtat
    24901 ttataagggg gctttcaaat gtcaggaaaa gatgtaaatg tcttcgagat ggcgcaatct
    24961 caagtaaaaa atgcatgtga taaattaggt atggaaccag cagtttatga attattaaaa
    25021 gaacctatga gagttataga ggtttcaatt ccagtaaaaa tggatgatgg ttctataaaa
    25081 acttttaaag gatttagatc acaacataat gatgcagtag ggccaacaaa aggtggaata
    25141 agattccatc aaaatgtttc aagagacgaa gtaaaagctt tatctatatg gatgactttc
    25201 aagtgttctg taacaggtat accatatggt ggaggtaaag gtggaataat agtagatcca
    25261 tcaactttat ctcaaggtga attagaaaga ttaagtagag gatacataga tggaatatat
    25321 aaattaatag gtgagaaagt tgacgttcct gcaccagatg taaatactaa tggtcaaata
    25381 atgtcttgga tggttgatga gtacaataaa ttaactggac aaagttctat aggtgttata
    25441 actggtaagc cagttgaatt tggtggttct ttaggaagaa cagctgctac tggatttggt
    25501 gttgctgtta ctgcaagaga agctgctgct aaattaggaa tagatatgaa aaaagctaaa
    25561 atagctgttc aaggtatagg aaacgttggt tcttatacag ttcttaactg tgaaaaactt
    25621 ggtggtactg ttgtagctat ggctgaatgg tgtaaatcag aaggttctta tgctatatac
    25681 aatgaaaatg gtttagatgg tcaagctatg ttagattata tgaaagaaca tggtaactta
    25741 ttaaacttcc caggagctaa gagaatatct ttagaagagt tctgggcttc agatgttgat
    25801 atagtaatac cagctgcatt agaaaactct ataactaaag aagttgctga atctataaaa
    25861 gctaaattag tttgtgaggc tgctaatgga ccaactactc cagaggctga tgaagtattt
    25921 gctgaaagag gaatagttct tactccagat atattaacta atgctggtgg agttacagtt
    25981 tcttacttcg agtgggtaca aaacttatat ggatactact ggtcagaaga agaagtagag
    26041 caaaaagaag aaatagctat ggttaaagca tttgaatcta tttggaaaat caaagaagag
    26101 tacaatgtta caatgagaga agctgcttac atgcattcaa ttaagaaagt tgctgaagct
    26161 atgaaattaa gaggatggta ctaagattta ctcttaataa ataataggat ataattattt
    26221 aagatataaa ggggctgtac atagtacagc ccttaaatta tgtggcttac aatgaagtaa
    26281 gctatttttg ttttaactta taaaaaactt gataaattat gtaatttaga atgtattttt
    26341 taccaccaca tgattacaaa ttagaagagt tagattagat ttttagacat gaacttatgc
    26401 attttaaaag caaagacctt aatttaaagt atctagtatt atttttaaaa atagtatatt
    26461 ggtttaatcc atttatatat ataatggaaa aatatatgga tttggattgt gaactttatt
    26521 gtgatgagag agttttaaaa aattgtactg tacaacaaaa gcaagaatat gctctaacta
    26581 ttgtaaattc aatgaaaaga agtacaaatt tttcaaataa atttgtagct ggattaaata
    26641 aacaatctga tattagaaaa agagtaataa acatgttcag tgaaaaacat aaaaatggta
    26701 tcttaagggc tttgattata agcttgtttt cttctattac attttttaaa ttcgattcaa
    26761 ctagtattat tgaagagaat gatttcttag agaggtcaag atatacgttt gaaggaaggc
    26821 aagaagacat gtttgtaaca atagatttta tatatgcaga tgtacctata aaacttagaa
    26881 aagagcatga agaaaactgt agagtcaatg gaatagtacc aaaagattca gattcaatag
    26941 aaattattcc tagattttat aaagattgta tggatttaaa aaaataagtt agacttaata
    27001 ttgatgttga tattatgaat tttttatatt taagcaattg aaaattttat tatagattca
    27061 attttatatg tgtttaagta tgaaatttat agatagttga aataattaaa tatgattgat
    27121 gcataggaat cattactaag ctataattaa ttataaggga gtttaaataa attatgagtt
    27181 acacaattat aaaatgataa aaaatagtta tctgttacaa aaaatgtatc tttttaatta
    27241 ataaagataa tatgaaaact taaaaggcaa ataaacttaa acataaaata ctcttaaata
    27301 agataggaga aaaatgtatg tctaacttta ttctaaatgg gatttttaga acagttatag
    27361 taagtagtct aagcattctt atgatattga ttttcaagaa aaatgtattt aaaagattta
    27421 gcaaaaaatt taattattat atatggatga tagttgttat taaattattt ttaccattta
    27481 catattatac ttttacaata aatatcttga gaagtaaaaa acacattaat attaataaca
    27541 taaatttaga gggatttaat aatgtttcaa ctataagtaa taatattata ttgtacactt
    27601 ggattataac tgtagtagtt tatttaattt acactatatt taaatatata aaacttaaaa
    27661 atttaataaa tgacttatct tatgatgttg atgatggaga gattgtaaat ctttataaaa
    27721 atatattaga agaatttaat ataacaaaag atataaaact caaatactct tatgaagttg
    27781 aaacaccagc attttttaat tcatgtgtgc ttttgccacc acatgagtat aaattgaaag
    27841 agttagactg gatttttaga catgagctta tgcattttaa gagtagggac ctttatttaa
    27901 aatatgtaat cctgttttta aaaacagtat attggtttaa tccatttata tatataatgg
    27961 ataaacatat agatttagat tgtgaacttt attgtgatga aagagtttta aaaaatcgaa
    28021 attcagagga aaaaaaggat tatgctttaa caattctaaa tgcaatgaga aaaggttcaa
    28081 atacttcaaa taaatttata gctggactcc ataagcaatc tgatattaaa aagagagtta
    28141 tgaatatgtt taatgaaaag tacaagaatg gtatattaat ggcattaact ttatgtttat
    28201 tatcttctat tacttttttg aaggttaatt caacgagtat tgttaatctt aatatattgc
    28261 caaaaggaat ggaagaaaaa gatgttacta atagtgcaat tgttacaatg atagaattta
    28321 catatgcaga agctcctaaa agtatgagag aagaacataa aataaattgt gaattcttag
    28381 gtttaatacc taaagactct gataaaatag gtgttggtat agaatattat gacagtctat
    28441 tgaatgatta aataaatatt attttaacag atattaatta taaagttttg aatagtaatt
    28501 tgttgagaaa taaatatatc cttataaaaa tagatactta tataccaaga ataaaaaata
    28561 gtcaagatag tacacaagga tatacttata aagaactcaa ataagtgaaa taaattatta
    28621 attataaggt ttataatttt aaaaaattag ataaattcag gtgtaaaata ctataaaaca
    28681 caattcggaa aagtgtatgt tgaacttcat ttaaaatgga atttcaaagt tgctatagta
    28741 agtagtttaa gcattcttct tatattaatc tttgagaata atgtatttaa aaaagttagt
    28801 aaaaaattta actattgtat attaatgata gttgctattg atatattatc taaaaaaatt
    28861 aagtatgaaa agattactgc taggacaatc ttttcatact taatttttaa tatttatatt
    28921 atccaaaatt ttcatctttt taacttagaa tttgagaatc actattagga aataggcttt
    28981 gatttatgta gggttaaaaa tttcacagaa tatctaactt tatactaaat ccatcaaaaa
    29041 atatacgcca tataaaagcg cataaaaaaa cacataaaac aatatttatg cgcactttaa
    29101 cctcgaatgg tacaaaattc tttactttta cagacccata ttatataatt aagtaaataa
    29161 tgtatattta ggtgacatat tggataagct aatttattta ttttattaat ataatgatac
    29221 tataaaagtt atataaagtg gtttacaaaa aagttacaga tagagctttt attaatgtat
    29281 tgtgtattag tattttatcc ttaggtacac ttcacctaca aaatagaatc ataagataat
    29341 tatgggaaaa ttttaggagg tttatcgtga agaaaaagat attaatacca gtatttgcat
    29401 cagttatggc attatcagta agttcaattg taaatgcaga tgaagtaaat gattcatctc
    29461 aaaataagga tgataaaaca aatactgaat taaatttagg tgaatataaa gaagtaaaat
    29521 ataaagttgc aaaaataaaa gatggagtag ctataaagat aagagaagaa ggtcaagttc
    29581 aaaacatagc ttattctgga gatgaattta cggttttagg aactcaagga gaatgggtta
    29641 aggttaaagt tgaagatggc gaaggttggt tagctactag atatgtagat atatctgaag
    29701 gtgtaggata tactaacgct gataaagtaa acttaagaaa agataagagt gaaagttcag
    29761 aagtaattga agagctggaa aaaggaagtt ctttattggt tttggaagat aatggagatt
    29821 ggttaaaagt taaagatggg gaaacagaag gatatgtaaa atcatcttat atatctgata
    29881 aagctcctgt aatagaagag ccacaagtaa acccaagtat tgaccaaaat gtagattcta
    29941 caaataataa tcaacaaaac aatgcaaata ctaatcaaca aaataataat tcaaatgtgc
    30001 caacagctaa ctctaatgca gttcaagctg tacttaactt ggcatattct aagcaaggtt
    30061 gtccatatgt ttggggagca gaaggaccaa acacttttga ctgttctgga tttacacaat
    30121 atgtgtatag aaatgcagta ggaaagaata taccaagaac atctaaagca caatctaaat
    30181 atggacaaac tgtaagtaaa gcaaatttac aaccaggaga cttagtattc ttcacaacta
    30241 atggttctgg ttctgttagt catgttggaa tatatgtagg tgggggtaat atgatacatt
    30301 caccatcaac aggaaaaaca gttagtgtaa ctagtataaa ttcatcatac tatacagcaa
    30361 gatttgttac agcaaaaaga atattataat cggtttgtaa tatgacaaat tgataaatac
    30421 ataaatatat ataaacgctc tatattatat ttaatttaat catggaaaca aaaaatgatt
    30481 atattttaaa tgtactatag agcgtttttg tatcacttta aatctaatca agattcaagt
    30541 taatatgact gttaaatagg agccttttca tatgatgatt atttgcgcaa tcccttgaca
    30601 ggatagggca aaggtggtaa gctactaaca gtacaaaaat acttttaatt ttagttcaga
    30661 gaggctaaaa aaggaggacg aaagatgtta aaatcaagaa atttaatcca gccagaagac
    30721 ttttcaatag aagaaataga tgaaatatta gaattagctc aaaaaataat tgacaatccg
    30781 tcaaaatatt ctcgtatatg tgaaggaaaa ctattagcta cattatttta tgaaccatca
    30841 acaagaacaa gattaagctt tgaatctgct atgaacagat taggaggacg tgtagtaggc
    30901 ttttcagagc caaattcatc ctctgcatca aaaggtgaaa ctctaggaga tacaatgagg
    30961 atagtatctg gttatgttga tataatagct atgagacatc ctcaatcagg tgcagcttca
    31021 gaagcagcta gatatacgga agtgccattt ataaatgcag gagacggcaa aaaccagcat
    31081 ccaactcaaa cacttacaga cttacttact ataaaatcat taaaaggaac tttagaaagc
    31141 catacaatag gattatgtgg tgatttaaaa tatggaagaa ctgtgcattc tttggttaag
    31201 gctatggcaa gatataaaaa tacaaagttt gtatttatag cgcctgaaga gttaaaaatg
    31261 ccagattata taaaagaagc tataaaggga catgcatatt atgaaactaa taatttggat
    31321 gatgttatag gaagcttgga tgttttatat atgactagag ttcaacaaga aagatttgaa
    31381 gataaatcag agtatgaaag acttaaaaac tattatattt taaataaagc taaattagaa
    31441 aaggcatcta aagatatgtt agtaatgcat ccattaccaa gagtaaatga aatagacata
    31501 gatgtagata gtgatgatag agctgtatat tttaaacaag ctaaatatgg aatgtatgtt
    31561 agaatggctt taataataaa acttttaggt ataaatgaag actaatagtt atcatataat
    31621 agtaaaacaa aggagatttt gggatgtata aaattctgga aaatatatat ataggcgaag
    31681 atatgtatag aatgaaagta aaaggtaatt ttgaaggtaa aatgggacaa ttctatatgt
    31741 taagagcatg ggatacttat ccagtacttt caagacctat aagtatacat gatatagatg
    31801 aagaagggat aacttttctt tataaagtag taggagaagg aactcaaatt ttgagtaacc
    31861 taaaggtaaa tgataatata aaattagaag gcccttatgg taatggatat gctaaggttg
    31921 atggaaaggt agctctagta ggaggaggaa tcggtgtagc tccactatat ttggttgcta
    31981 aaaatataaa aaattgtgat gcatatcttg ggtttagaga agatgttata ttagaagatg
    32041 aatataaaca agtatgtaat aaagtctata caacagtagg aaatactttt gtaacagata
    32101 taattgatgt tgagaaatat gattatatat taacatgtgg tccaactcca atgatggaaa
    32161 aattagttaa gatggtagaa ggtactaaaa cgagaattat ggtatctctt gaaaaccata
    32221 tggcttgtgg agtaggtgcc tgtttagtat gtacgtgtaa aactaatgga ggaaataaaa
    32281 agacttgtaa agatggtcca gtgttctggg gagaggacgt gatttttaat gggtaattta
    32341 agtgtgaaat ttggaagtgt agaatttaaa aatccagtaa taatggcgtc tggaactttt
    32401 ggatttggaa aagaatataa tgagatttat gatatacaaa aacttggtgg tataagtagc
    32461 aagggtctta ctttaaataa aaaacctggt aacaatggta tgagagttca tgagacatca
    32521 tccggtatga tgaatagtgt tgggcttgaa aaccctggag ttcaaggatt tatagactat
    32581 gaattacctt tttttagtaa actagactta gtcagaatag ctaatgttgg aggtgggaca
    32641 ttagaagact acttattagg ggttcaaatg ttaaatgaca agcctataga cattattgaa
    32701 ttaaatatat cttgcccaaa tgtcaaagct ggtggaatgg cttttggtat aaaaaatgaa
    32761 gtggcaagag aagttgttcg tgaggtaaga aacataacga aactgccttt agtaataaaa
    32821 ttatcaccca atgcagaaga tattgtagga atggcaaaag tttgtgaaga agaaggtgct
    32881 gatggagttt cattagttaa tacttttaaa gcaatggcaa ttgatataaa aaatagaaga
    32941 ccagtatttg aaaatgtata tgctgggtta tctggaccag ctataaaacc tatagctttg
    33001 agaatggtac atgaagtgtg taaaaatgta aatatacctg taatgggaat gggcggaata
    33061 actaaagcca cagatgctat agagtttata atggcaggag ctacttgtat ccaagttgga
    33121 actgcaaatt ttatgaatcc tagaataggt attgaaataa tagatggaat taatgaattt
    33181 atggatagag aaggaataaa aagtttagac gaaataagag gaattatata aggtagaata
    33241 aatagtattt taaagataaa aaatatagat taaataaaca ataattttat aacattaaca
    33301 tggaggaaaa ataatatgag taaagtagat gtagtggata tattaaagaa aagtgatgca
    33361 ttgttggagg gacatttttt attatcttca ggaaaacaca gtaatagata cgtacaatgt
    33421 gcaaaagtat taagatttcc agaatatgct gctgaggtat taagtacagt tgttgaacaa
    33481 ataaaagact tagatataga cttagtagta ggaccagcta tgggtggagt aatagtttct
    33541 tatgagttag gaagacaatt aggaaaagaa gctgtattta ctgagagaaa agacaataca
    33601 atggagttaa gaagaggatt tgaagttaaa aaaggagcaa agataataat tgctgaagac
    33661 gttgtaacta ctggtaaatc aactatggag acaaaaagag tattagaagc cttaggtgga
    33721 gaagttgtag gtgttgcatg tatagcagat agaactaatc atgatatagg tatgcctata
    33781 tatagtgcta taaaacttga tattcaagtt tatgaatctg atgagtgtcc tttatgtaag
    33841 gaaggaaaat taccagttgt taagcctgga agtagagagt tcaaggaatt agggatgtaa
    33901 taaaaactta attatttata gtgttactta aaaaatgtaa attttttaga gtaaaaattt
    33961 gtaatataac atataactta aaaataaaaa gcagttagcc ttataaatga aggttagctg
    34021 tttttttgtt aaaaatatat ttacaaaata agagatagta gcagtacaat ataagtataa
    34081 ggtaaaagta ttatatatta gggaggggta agtatgaaaa atcaattaaa tgaaaatcat
    34141 aaaaaggttg tattttttgg agtaggtgct gtaggagcta cttttgcaga acaatttttt
    34201 aactctaaat atgattttaa aattctttgt gataatgaaa gaaagaaaag atatttagaa
    34261 gaaggattta taataaatgg aaaaaggtat gattttgatt atgtaactaa agatgagtat
    34321 aaacaagagg ctgattttat aattataggt ctaaaatata ataatttaaa agaaaatata
    34381 aaagaattag atggattagt tggaaaaaat acagttataa tgtctctgct aaatggagtt
    34441 gatagcgaag agataatagg agaaagattt ggaattgaaa aaatggtata ttcatatgtt
    34501 accaatatag atgcaaagaa aatcaataat aatattatac acactactaa tgggataatt
    34561 gtatttggta acaaagataa tagtgaagat agaaaaacta atataataac cgaagtcttt
    34621 gatgatgtaa atatagaata tactttatca aaagatattc aacgagatat gtggtggaag
    34681 tacatggtta atattggtgt aaatcaaact tcagctatac ttggtgcacc ttatggagtt
    34741 tttcagagtt ctgagcattt aagagaatta gcaaaatctg caatgaggga agttgttgct
    34801 atagcacaag caaaagacat atctcttaca gaagatgatg tggaacattc attacataga
    34861 atactagaac attcaaaaga aggaagaaca tcaatgcttc aagatgtgga agctcataga
    34921 cttacagaag tagatatgtt ttctaagaat atctgcaaac ttggaaaaaa atataacata
    34981 cctactccta taaatcagac tttcttttat atgataaaag taattgaaag tagattttaa
    35041 agggtctttg taagaaatat tgataattat tgattttaaa ttttattaat gttatatact
    35101 atttaagtta ctaaatattt ttaactcttc aatgaaaata gttgctgctt ttgataaagt
    35161 tatatctttt ggaaatatat atgagaaatg tctgttgtag ctgcttccaa gctctatgac
    35221 agacagctca ttgtttagaa caggtaagct tgtcactaga ttagagacta ttgtaattcc
    35281 taaattattt ctaacagatt ctttgacagc ataattactt ccaaaaacca tcatactttt
    35341 tggaataatt tctttaacac ttaaaaacat atctaaataa tctctggttc cagaaccatc
    35401 ctccctgact atccattttt ggttttgaag tttatcaaaa ctaaaatctt ttaataaatg
    35461 actcttgtat ggaagtgcta aaaccatctt atcttcaaaa aaatattctt gaataaatga
    35521 aggggaagag caagtgcctt caataagacc aatgtctaaa atataatcct taacatgaga
    35581 agatacgatt gatgtatttt ttataaaaac ttctacatct atgtcaggat actttttaga
    35641 aaataatgct agaaaatttg gtaaaatata ctctccaata gttaaacttg caccaatctt
    35701 taagtgacca gttatagcat ctgaaacttg gcttacatca tgataagtaa tatttaaaag
    35761 atttaatatt tctttagctc ttttgtacaa gatttgtcca ctttctgtta taaaaatggt
    35821 tttttgttta atagacctgt ttataagcac aactttaaaa taattttcta gattttttat
    35881 gtgtttactt acacttggtt gtgatagatt taaatattcc ccagctttag taaagttttt
    35941 gtattcaaca acagctacaa atgtttttaa ttcttcaaac aattttttgt ctcctttacc
    36001 taatgaaatt ttacattttt tatatacttt ctacagataa ttatactata tataataacc
    36061 attaaaaaca ttaatcatta cgatattata tatttatttt acttatggtt attacaatta
    36121 tatactattg ctatgaagaa aactatatta attataaggt aggtatatta tgttaaagaa
    36181 aaaaagcagt ttgacattaa aaactataag ggaaatatta ccaggactgt ttgtatcagt
    36241 attagtaggt tatattagta tatttatttc tattttaatt cctaaggtag gggcagcatc
    36301 aatttctata tttttaggta tgtttgtagg aaacctattt ttaaatcaaa aggtatttca
    36361 aaaaggttat aaattttcag agacagattt gttatcatat tcaatagtac tattaggagc
    36421 aacactcagt gtatctacct taatagactt aaaagtttca ggaattttat ttataatatt
    36481 gcaaatgaca ataacaataa tagctgcact ttatataggt aaaaaactag gatttgaaga
    36541 aaactttaga tttctaatgg caagtggaaa tgcagtatgt ggttcttctg caataggagc
    36601 aacagttcca gtggttaatg cgacagataa agaaaaggga attgcagtaa ctattgtaaa
    36661 tgttactggt atatttctta tgtttttact tccaataata tctcaatttt tatatagcca
    36721 tgaacttgta aaaacttcag caatgatagg tggaactttg caatcaattg gtcaggttgt
    36781 agcaagtgga gcaattgttg gagaaaatgt aaaagatttg gctactattt ttaagatagt
    36841 aagagtaata tttctagttg ttgttgtatt agtatttgga catatgaaaa atcaatctaa
    36901 tagggaaatt cttgaagaag aaaaggatga gataaaaaag aaaaaagtga ctataccatg
    36961 gtatgtaatt ggattcttta taacttgtgc acttttttca atgaacatta taccaaaaga
    37021 agtatctgtg ttgtgcaaag aaataagtaa caaactagaa ataatagctt tagcagcaat
    37081 tggattaaag gttaatgtaa aagatttagt gaaacaaggg aaagaagttt ctttatatgg
    37141 tttatttgta ggtatagtac aagttgtatc agctgtagtt ttaataaaaa tatttatata
    37201 gcataaaaat agtatttgta atattaattt gattaactaa aataatatga tactaataat
    37261 ttataatata aataaaaaat gaatttcgat aagtttaagt gggtataaac ttatagaaat
    37321 tcatttttta tttaaggtaa aaatttaaag taaaatatta ttttacataa taataacctt
    37381 ataaattttg aaattactta tctttgtgtt aaaataagta aaatgcacat attagagcta
    37441 agtttttaag taaataaaca aaatgaaaat ataaatatta aggagatttg gtatatgaat
    37501 gtttatgaaa aaggtaatgg agttatacta gaaggagtaa cagattttga cccagtacat
    37561 atatttgagt gtggtcaatg ttttagatgg cacaaacaag aagatggctc atacactgga
    37621 gttgcaaagg gaagaatatt aaatgtcaaa aaagagaatg ataaaatata tttaaataat
    37681 actaatttaa aggagtttaa cagcatttgg tataattact ttgatttagg aactgattat
    37741 acagaaataa agaataagct aaaaaatatg gatgaatatt taaataaggc tactgaattt
    37801 ggatggggaa taagaatatt aagacaagat ggatgggaaa tgcttatatc atttataatt
    37861 tcctctaata ataggattcc tatgatacaa agagcgatag aaaatttatc aagaaagttt
    37921 ggtaagtata taggtgaata tgaaggtaat gaatactatg cattcccaac accagaagaa
    37981 ctaaataagg cctcacaaga ggaaataaga gcatgtcaaa caggttttag agataagtac
    38041 ataaaaagta ctacacaggc agttatagag aataatgatg aggtatctga atatacaaat
    38101 ttaagtacag aggattgtag aaaagagctt ttaaaattta atggagtagg accaaaagta
    38161 tgtgattgta ttgcattatt tggtatgcaa aaatatgatt cttttccagt tgatgtttgg
    38221 gttaagagag ttatgcaaga attttatata gatgaagaca tgagtttacc taaaatgaga
    38281 acttatggaa tcgataaatt taaagaaatg tctggatttg ctcaacagta cttattttac
    38341 tatgcaagag aactcggtat aggaaaataa attaaggggg agaaattatg ggagtaatag
    38401 gaacaatatt tctgttctat ttaattatat catatctagc tggagccata atttctgtga
    38461 ttatattgct tgaaaataga gaccctgcaa aaacaatgtc ttggctattg atgtttatta
    38521 tatttccagg tgtaggttta atgatatatg ctatttcagg tagaaatatt agaaaaagaa
    38581 aattatttaa gactcaaaaa ctagctaata atataaaaga aaagaaacta tttgatacat
    38641 tagaaaagat aacagaaata gttgaattag aaaaagaatc tataaaacaa aataagcttt
    38701 taagagatga agaggatggt agttatagaa aaagggtaat aaatatgttg ttaaagactg
    38761 gtatgtttcc ctttactaaa aataataaag tggatgtatt tgtagatgga aatgaaaagt
    38821 ttaaaagact tatagaagat ataagagaag ctaaagacca tattcattta gagtacttta
    38881 tcatcaaaga tagtgaaata ggtagggtgt taaaagaaga acttataaaa aaggctaaag
    38941 aaggcattaa gataagaatt ttatatgatg atgtaggatg ctggagattt tggtttaata
    39001 gaaaattttt tcgagaaatg agggaagtag gtatagaaat tgcagcattt ttacctacaa
    39061 aatttcctat aataggaggg aaattaaact atagaaatca cagaaaaatt gttgttattg
    39121 atggaataat tggatataca ggtggaataa atataggaga tgaatattta ggcaaaaatg
    39181 ataagtttgg atattggaga gatactcata ttagaattaa gggtatttct gtatacatgc
    39241 ttcaaatgac atttctgatt gattggtatt atacgacaaa agaagtatta gtgactaaaa
    39301 attatttccc aagtgtaggg aatgttggtg aaagtatgat acaagttgtt gcgagtggtc
    39361 cagacagtga ctgggaagat attcattatg cttatttttc tgcaatatgt caggctagaa
    39421 aaaatgttta tatagaaact ccatatttta ttccagatga aagtctacta aaagctataa
    39481 agagtgctgc tcttagtgga gttgatgtga gaataatatt tcctaaaatt gcagaccaca
    39541 agatagtaaa tatagcatct tattcatatt ttgaagaaat attaagagca ggtggtaaag
    39601 tttatttata taataaagga tttatacatt ctaaagtcgt aataatagat gataaaattg
    39661 cttctgcagg tacagctaat atggatttaa gaagttttat gcttaacttt gaggtaaatg
    39721 catttatcta tgatgaagaa gttattaggg taatgacaga tgatttcttt gaggatttaa
    39781 gtcactgtga agaacttaat ttggaggtat ttaagaatag aaatatcata caaaaaatca
    39841 aagaatctgt agcaagatta ttttcaccaa tattataaaa aattattaaa aaaggtattg
    39901 aaaattatgt gtgattggta tattataata attgttagca ctctattaga tagagtgata
    39961 ataaataaaa agtatatata taaatctaca tatataagta gtattaattt aaaaataact
    40021 aaaaacatag gcgaactagt ctatacttaa tattttagga ggcgtgaata atgaaaataa
    40081 gaccattagc tgacagagta gtaattaaaa aagtagaagc agaagaaaaa actgcaagtg
    40141 gaatagtttt accaggagca gctaaagagc aacctcaaat agctgaagtt gtagaagttg
    40201 gaccaggtgg aatagttgaa gggaaagaaa taaaaatgga attgacagta ggagataagg
    40261 ttatattcca aaaatattct ggaacagaag ttaagataga aggacaagaa tacacaatac
    40321 taagacagag tgatgtatta gctgttattg aataaatata gaataaattt attaggaggg
    40381 gtttaaaatg gctaaagaaa ttaaattttc agaggaaaca agaagagctt tagaagctgg
    40441 tgtaaataaa ttagcagata cagtaaaagt aacattagga cctaaaggaa gaaatgttat
    40501 attagataaa aaatttggtt ctccacttat aactaatgat ggagtaacta tagcaaaaga
    40561 gatagaacta gaagatagat ttgaaaatat gggtgctcaa ttagttaaag aagtagcaac
    40621 taaaactaat gatgtagctg gagatggtac tacaactgct acagttttag cacaagctat
    40681 aataagagaa ggtttaaaga atgtaacagc aggggctaac ccaatacttt taagaaaagg
    40741 aatacaaaaa gcagtaacag tagcagtaga agaattaaaa aatcaatcaa gaatagtaga
    40801 aacacaagag gctatatctc aagttgcttc tatatctgct ggtgatgaag aagttggaaa
    40861 attaatagca gaagctatgg aaatagtagg taaagatgga gttataactg ttgaagagtc
    40921 tcaaactatg aatactgaat tagatgctgt tgaaggtatg cagtttgata gaggatttgt
    40981 ttctgcatat atggttacag atgtagataa aatggaagca gttttaaacg acccatatat
    41041 attaattact gataaaaaaa tatctaacat acaagaatta ttaccagttc ttgaacaaat
    41101 agttcaacaa ggtaaaaagt tattgataat agctgaagat gtagaaggtg aagcattatc
    41161 tacattagta gtaaataaat taagaggaac atttgatgta gtagcagtta aagctccagg
    41221 ctttggagat agaagaaaag aaatgcttca agatatagca atacttacag gtgctcaagt
    41281 aatatcagaa gaattaggtt atgatttaaa agaagctgat ttatctatgc taggtagagc
    41341 ttcatctgtt aaggtaacta aagaaagtac tacaatagta gatggttctg gagataaaaa
    41401 agctatagaa gatagagtaa ctcaaattaa acatcaagta gagcaaacta cttcagattt
    41461 tgatagagag aaattaatgg aaagattagc taaacttgct ggaggagtag ctgttgtaaa
    41521 agttggagct gctacagaag tagagttaaa agaaagaaag ttaagaatag aagatgctct
    41581 taatgcaact agagcagctg tagaagaagg aatagtagct ggtggaggaa ctgcttttgt
    41641 tagtgttata ccagcaatag gaacactaat tgagagttta gaaggagaag ttaaactagg
    41701 tgctcaaata gttaaaaaag cgttagaaga gccattaaga caaatagcta taaatgctgg
    41761 tcttgaaggt gctgtaattg tgcaaaatgt tgttaattct gaagcagaaa ctggatttga
    41821 cgcattgaat gaaaaatatg taaatatgat agaagctggt atagttgacc caactaaagt
    41881 tagtagaagt gctttacaaa atgctgcatc aatagcaagt actttcttaa ctactgaatc
    41941 tgctgttgct gaccttcctg aaaaagaaga tgcaggaatg ccaggaatgg gtggcggaat
    42001 gcctggtatg atgtaattta aaaaatttaa aatataaatt ttaatataaa gaactatttc
    42061 gacttagatt ttaaactcta agttgaaata gttcttttgt atatatctaa tttttgtata
    42121 tctataaatt caatacacat atttagataa tcagtatcta gtttgtaata atattttaga
    42181 gtagtattgt tattttttag gttatgattt tagatatata ttaattaagg tataattgtt
    42241 gatagagaat aaatcaaatt taaaaggatg cgataatatg gacttaagaa aattgcttga
    42301 agaagttaaa aatgaaaata ttgatataga ttttgcgtta gaaaagttaa aagatttacc
    42361 ttatgaagac cttggatatg caaatataga ccatcataga gaattaagaa atgggtatcc
    42421 ggaagttatt tattgtgaag gtaagagtga tgagcatatt ttagggatta ttgataaaat
    42481 gaatcaaaaa agttctaaca tattggggac aaggtgcaga aaagaaactt ttctgaaaat
    42541 taaagaaatc tataatcatg ctgagtacga agaggcgtct aaaattctta agattcaaaa
    42601 tcatgatata gagaatatag gaaaaggtaa aattgtgatt gctacagggg gaacttctga
    42661 tataccagta gcagacgaag cttatcatac agctaagttt ttaggtaatg atgttgatag
    42721 aatatatgat gttggagtgg caggtataca taggctttta aataaaagac ataaaataga
    42781 tagtgctaga gtaatagtag ccgttgcagg tatggaaggt gctcttgcaa gtgtaattgg
    42841 tggactggtt gatgttccag taatagctgt tccaacttct gttggatatg gagcaaattt
    42901 tggaggatta gcagcattgc tagctatgtt aaatagctgt gcatctggaa tatctgttgt
    42961 aaatattgat aatggatttg gagcaggata tttagcatca atgataaata aactgtaaat
    43021 acattgatat aaattaaaca aaactatata ataaaattta atttctatta tatttttact
    43081 attaaaattg aggagacata tatgagtgaa aaaatacttt attttgatat aataagcggt
    43141 atatcagggg atatgaccct tgcaagttta ttaaatttag gtgtgcctaa ggaaattttt
    43201 ttggaggaaa ttcataaact aagaatgagc gatgaattta atataaacat cagctcgaaa
    43261 actgaaaatg gaatagtagg tacaaaagta gaagtaatta caaaagaaaa acatacacat
    43321 agaaatttag tggatatatt tgaggttata gataaaagca ctctaaacga aaatgtaaaa
    43381 tctaaagcaa aaaaaatatt tatggtaatt ggagaagcag aagcaaaagt tcatggaact
    43441 acaatagaca agatacattt tcatgaggta ggggctatag attcaattgt agatatagta
    43501 ggagcttcta tattagttga cttactttgt gtagataaag tttgtgctag tacagtgtct
    43561 gttggttcag gatttgtaaa atgtgaacat ggaattatac ctatacctgc acctgcaact
    43621 atagaaattt taaaaggtgt gccaattaaa ttaaataatg taaatggaga atgtacaact
    43681 ccaacaggag ctgcaatcat aaaagttttg tgtgatgagt ttgtagatga gcttgaattt
    43741 tctccaaagc aaattggata tggtataggt cataagaagt ttgaagtacc aaatatactc
    43801 agaactgtat taggtgaaaa aaaaaagaag aattaattta tgaaattagt gcaaacattg
    43861 atgatatgtc ttcagaaatc tattcttatt tgtatgaaaa aatattagat gagaaagcat
    43921 tggatattta tacagagagc atttatatga agaagaatag accagcaaca aaagtatcaa
    43981 ttttatgtag tgaatcagat ttagaaaaat ttattaaaat attactttta gaaactagta
    44041 cttttggagt gagatataat gcatataata gagaaattct agagagaaaa tttgtaaaat
    44101 tagatacaaa atatggagtg gttacagtaa aactagggta ttataaagga aaactagtca
    44161 aggctactcc agaatataat gaatgtaaaa ctatagcaaa aaatgaagat attcctataa
    44221 aaaaagtgtt tgctgatata aattttagaa taaaagaaga atttagtgaa aacttattga
    44281 cataagtaat aaattttgat aaattaatat tcagccttat taaataaaag taaacagaaa
    44341 aaaatatata aaatataaac tcactcgtat atgctcagaa atatggtctg agagtctcta
    44401 ccaagatacc gtaaatatct tgactatgag tgaaattatt ataccaaaag acctagttaa
    44461 gtaaatgtgt aggctgagtt aaactcccta tgtgttggct ataagataaa ggtattttgg
    44521 aagcctgtat agtaatttca cttttgctag aaattatgga agttactata caggtttttt
    44581 gtgtaaaaag atttaatata atttagactg tattgtgtta aaataaatat ataaaaacta
    44641 tattatacaa ggagagatgt gaatttatga aacatgaatt agtacttgta atagattttg
    44701 gaggacaata taatcagttg atagctagaa gagttagaga aaacaacgta tattgtgaaa
    44761 tacttccatg tactgcaagc atagaaagaa taaaagaaaa aaatcctaaa gggataatat
    44821 ttacaggcgg tccaaatagt gcgtatttag aagattctcc aacaatatca aaagaaatat
    44881 ttgaattagg agtaccaata cttggaatat gctatggtat tcaaataatg tctcatgtat
    44941 tgggtggagt tgtaagaaaa ggtaataaac aagaaaaaga atatggaaaa acagctataa
    45001 cttatggaaa atcatcatta tttgaaggaa taactacaaa tagtgtatgg atgagtcata
    45061 cagatttaat agaaaaagtt ccagaaggat ttactatagt tgcaaatact aatgattgtc
    45121 cagtagcagc tatggaaaat gtagaacgaa acctatatgg agttcaattc catccagagg
    45181 tggaacactg tttggaaggg gataagatac ttacaaactt cttatataat atttgtaaag
    45241 taaaaggtga ctggactaca gattcattca tagaagataa aataaaagaa ttaaaagaaa
    45301 aaataggcga taaaaaagct ttatgtgctt taagtggagg agttgattct tctgttgctg
    45361 cagttcttat tcataaagca attggggaca acttaacttg tatatttgtt gaccatggtt
    45421 tacttagaaa aaatgaagga aatgatgtag aaagaatatt tagagagaaa tttgatataa
    45481 atttaataag agttaatgca gaagatagat tcttatctaa attaaaagga gtttctgaac
    45541 cagaagctaa gagaaaaata ataggtgaag aatttataag agtatttgaa gaagaatcaa
    45601 ataaattagg aaaaatggat ttcttagtac aaggaacaat ctatccagat gttatagaaa
    45661 gtggacatgg aaatgctgct actataaagt ctcatcataa tgttggtgga atcccagaag
    45721 atgtagactt ccaagaaatt gtagaaccat taagagagtt atttaaggat gaagttagaa
    45781 aaataggatt agagcttggt atagaagaag gattaatatt tagacatcct ttcccaggac
    45841 caggtctagg aataagagtt ataggtgatg taactaaaga aaaatgtgat atattaagag
    45901 aagctgatgc tgtttatatg gacgagctta gaaaagcagg tttatacaga gaaatatggc
    45961 aagcttttgc aacattacca gatgttaaga ctgttggagt tatgggtgat gaaagaacat
    46021 atgcttattt agttggttta agagcagtta cttcttctga tggaatgact tctgactggt
    46081 ataagatgcc ttatgatgta ttagaaagaa tttctaatag aataataaat gaagttgatg
    46141 gagtaaatag agtagtttat gatataactt ctaaacctcc tggaactatt gaatgggaat
    46201 agtaaataaa aaagtcgtaa gccttgtatt ttcaaggttt gcgactttta aaaaaatctt
    46261 tgtgatacta aaatgatact atatatttaa attatctagt ttttgggcaa cttcaagttg
    46321 cttatttggg tataaatgtg aatatgtatt ccaagttgtt tctacttttt catgccccaa
    46381 tctttctgct atagttaata tatttacatc catatttacc aataatgacg catgagaatg
    46441 tctcaaatca tgcactctaa ttctttttac attagataac ttacagcatc tatctagctc
    46501 tttagaaaga taacttttag caaatttaaa tattctttca ttatctttta agtcatatag
    46561 cttagataga taatctttta tattattata taagaaatgt gggataggga caacacgttt
    46621 acttttagga gttttaggag aagaaacaat atcctctcca tttagtctta tgtagctttt
    46681 ttcgacgctt attttatttt cgaaaatatc ttttggagta agagctaaca actcccctag
    46741 tctaagacct gtccaaaata aaatttcaaa tgctagtcta ggttctgatt tcttttcaaa
    46801 ttcaataaat tttttaaact cttctaatgt ccaaaagttc atttcatctg cgtttttttt
    46861 accgatagaa cctgccaaat gagcaggatt agaaggtaag ttataatatt taactgcata
    46921 atttaataca gcaacaagct ggttgtttat cgttttaata tatgtttggc tataatcact
    46981 ttttaataat tcgttttgcc atcttcttat atgtgttgca gtaatttcat ttatttttag
    47041 acttttaaaa aaaggtaaaa ttctaaagtt tattaaatac tttttagttt ctaatgtaga
    47101 caattttaat ctagaagaca tatcgtgcat gtattcttct atgagacttt caaaactcat
    47161 atcagtactc attttagatt tattcaaaaa ttctctttca aactctagag cttctttttt
    47221 agttttaaaa cctctcttaa ttttcttttt cctatcccca tcgaagtctg taaaataaaa
    47281 gctagcatac caactttttc tttgttcgtc tttgtaagca ggcattttag acacccccaa
    47341 ttatgtcatt ttaatattac aataattata acatgttaga tatgctatct aaataattgt
    47401 tttattttcc tcttcttttt atttcttgaa gcttgtaata tttacacagt tctaagaagt
    47461 cttcttctag tgctatagat aagtctaata tcgtagatat acctatatct ttgtactctt
    47521 gattttctag ttttgatata taacttctgt tacgtctcat tttttctgct aattctagtt
    47581 gtgtcattct tttctttttt cttaattttt tcaacattta ttaaaatcca ccttttaatt
    47641 atttttatga catttttata attaatagtt tgtgtaattt cttgtaaaaa atgttcctca
    47701 ttagaacatt ttttggtgga aaaatgtgtt aaaatgtagt taagaaatta aaagcaaaaa
    47761 gcactataac aatagtgcta aaatggtaga gtttaaagaa aaaataaatt ttatttaaaa
    47821 gcttataaat ataaagtaaa taatttgtaa attaaatcta agaataatgt aaaaaatgca
    47881 ataaaaaaat aaaaattaaa attatgaaaa ataaagctag tcgactaact aaaaatagaa
    47941 atatacagat aatctaaaat actagaaata aaaaaataga agtgaatttt
//
LOCUS       DAMJVK010000020        47106 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.020, whole genome shotgun sequence.
ACCESSION   DAMJVK010000020 DAMJVK010000000
VERSION     DAMJVK010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 47106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47106
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.020"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(197..445)
                     /locus_tag="PC414_001122"
     CDS             complement(197..445)
                     /locus_tag="PC414_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF2463529.1"
                     /translation="MQKTKAWGIIEPEKPESQQQMAHMDFAVKDLEEAVQYAIHCGAT
                     IAEEQFTDDWRVMIDPAGHPFCLCQMKSIMESSHFSLL"
     gene            complement(476..937)
                     /locus_tag="PC414_001123"
     CDS             complement(476..937)
                     /locus_tag="PC414_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HDF2463530.1"
                     /translation="MKFGGVLLIVKDINKSKKFFEDVMEQKITLDLGEHVSFENGFGL
                     QSNYKAIIGYDLPIYQQSNNFQLYFEVNDIKQWESKINRIGNIEFLHYIKEYPWGQRT
                     FRFYDFDKNIIEISESMESVIKRLLKQGLALEEISKRTMYPVEFITQFQQV"
     gene            complement(975..1808)
                     /locus_tag="PC414_001124"
     CDS             complement(975..1808)
                     /locus_tag="PC414_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2463531.1"
                     /translation="MLKKESIHFINKNRSLIVCEDNFVYLLPCIQLRNWISNFTISFP
                     NQTIISDNYTIIPHGSVTLVFFYDATGLHSLLFGPTTKPKTVGNIANRCDVILIIEFQ
                     PAGFFPLIGIQQSELIDKVVPFSIINTSLDLEIKKIFNESLSIDKLILKLEELLISNI
                     KIEYSYEFILAIQLIIQNSGNISSQEISKKVFYSSRHLNRLFNQYLGLSMKSFSRLVR
                     INKSIKLLNNNKTSWMSICNELGFYDIPHFIKDFKIVCGITPQKYRANMSDFYSEIAK
                     F"
     gene            complement(2082..2768)
                     /locus_tag="PC414_001125"
     CDS             complement(2082..2768)
                     /locus_tag="PC414_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2463532.1"
                     /translation="MKITHNSLAVEAKNIIKEYKIGNTTTRVLKEVSLQVMKGEFVSI
                     MGQSGSGKSTLLYILGGLDTPTSGKVYMNGADISHFNDEKMSIIRRRNIGFVFQFYNL
                     IPNLNVEENIMLPLLLDGKNLKDYKNQLDEILDIVGLTDRRKHTPRELSGGQQQRVAI
                     ARALIGKPEILFADEPTGNLDSKTGIEIIDLLNKINRDNGQTIIMVTHSPEAAKSSSR
                     TITVSDGLIV"
     gene            complement(2783..5260)
                     /locus_tag="PC414_001126"
     CDS             complement(2783..5260)
                     /locus_tag="PC414_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2463533.1"
                     /translation="MKIILKYIFTNIKERKIRTAVMLLSIVLSTVLLFVSFSIGLSYE
                     SAQRKMAKGMYGTATISVQSKNPDILTNLEDIPDLNTIKSKVGVLESSAIYNKGGYYE
                     EFSLISADLSQLNKINKPRLENGDSITDFSGDKIILPNRFTSKYKIKKGDSITLQIYG
                     KSYTFQVSDIASYDTVFLRNTRGVNALLPKETLSKIINKGSGYTRVLIESEEEITENL
                     VNKLSEELSTEKYMVSNTINETKIISDARQKTMPFFLISFFALTLSIFIIYSSYKVIT
                     LERLPFIGTFRSIGANEKTVTRILMLESILYGSIGGLIAIPIGVVVLNLMLHGLGNSL
                     EQGISIPVVISPIGVIISVIVAIIVSSFSAYIPVKKASHLPIKNIVLGTVEEKNVSNR
                     SILFIGSIMFILSILLPRISPENTLYLAGGFSLLGLIVATIVLIPLITDIMSIVFEFV
                     YKNILGNEGKLAARNMKNNKNIIQNITLLFISISAVIAISVVGNFVKTYITDVFRDAE
                     LQGFADGNMNEEFIEDVRHMEGIKKILPLYVMNNEISGNGVTLSRLEGTDNIKLYNSM
                     FGINYTNFEIKKQVIEAFNDKRSVILNEDTLKKVGLSIGDTITLSNDKYDFSYKIVGS
                     FKSRANDVEAVIPSHYAVNDFDKTNYGFLVYTAVNPDAVMIQIRYLFGDTYNWSRTVE
                     EFNNDSLNTISSFLSPMNKMTYFIFLLATVGIINNLLINYIQKRRSIAMYKSIGLSNK
                     QNIKVTLIEGVTSGLLGAVIGIVISILEIQTIFIVAGPKISMKPDLDFKTFIIVGLLG
                     IIVTLIGSIVPIIKGKKMKLIEEIKFE"
     gene            complement(5479..6882)
                     /locus_tag="PC414_001127"
     CDS             complement(5479..6882)
                     /locus_tag="PC414_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2463534.1"
                     /translation="MHKPSYKTVFHSYIIFFMIFICLILTVIGIFLSLITIQKPNGEF
                     IRSDWPKKFTENFNEQIVFIDNKPQVKQSGLEKLRENQLWLQILDSSGNRVYGFLEPK
                     EHKDYYSNSELLALANQNNSSNGTVYFNTIKNNKNDYIYIIYFPLNISKVNMFFDGGK
                     FIGGKSIVFSIIGIIFLIILVSGIAYGYWVTKCISNITSAVSDISKRSYLPVNIKGVF
                     ADIYNSLNILNEEIHKSDEIQNKTDIMREEWISNITHDLKTPLSPIKGYAELLTDSST
                     NYSPEKVYKYASIMLKNIEYTNTLINDLKLTYQLKNGIIPLNKNNYDLVRFLKELSID
                     ILNYPEYESNIVEFKSNIDNINFDFDCTLLKRAFSNLIVNAFTYGDKNTKVCVHVTLN
                     ERIEIIISDNGKGMSREEASNLFNRYYRGSNTKQKIEGTGLGLAITKQIIEVHGGNIS
                     VESIPGLGSSFYITFPI"
     gene            7381..8241
                     /locus_tag="PC414_001128"
     CDS             7381..8241
                     /locus_tag="PC414_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF2463535.1"
                     /translation="MKKQKSPILLEMLGLFFGCISMSIGINMFLKPHTIAPGGLSGLS
                     LVLNKVTGLPVSAIMLIIGVPLVILAFRIMGTKNSLKTLFGTVVFSAIVQLTDPLSKL
                     RFTNDLLLSSIAGGILVGIGLGIMFKSDASTGGTDLIALILSKKFPGIKATKFMSCLD
                     GMVVISSGIVNRSLETALYSGIALCVLIKIADMIMEGFDHSKAFFIISDEPESLRQAI
                     TEELDRGLTILDGKGGYTRENKEVLLVVVSKKQELYLKRLVKRTDPTAFVIVTDVHEV
                     LGEGFKNLEN"
     gene            complement(8480..9037)
                     /locus_tag="PC414_001129"
     CDS             complement(8480..9037)
                     /locus_tag="PC414_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2463536.1"
                     /translation="MSDSEYPQMKTLETRSCILRPASLDDVEDFFDCYKNKIVVKHLP
                     FSEHKSLEDTRKFIKSFFLNPYKKGKIGHFAIVYKRDNKVIGNMGFNNINPKALEAEI
                     GICINPSYWGHDFATEITKRVIQYGFRELNLNKIIAITYEENQNSTKSLDLLGFKCTG
                     KYIKKIHTGNTVKNVPCYQYELKKQ"
     gene            complement(9227..10114)
                     /locus_tag="PC414_001130"
     CDS             complement(9227..10114)
                     /locus_tag="PC414_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2463537.1"
                     /translation="MNIKLELYKVFNAVVNNKSFSLAAKELFMSQPAVSQSIKQLEEQ
                     LDTLLFYRNNKGVKLTPEGKILSEHVTTALKLISSGEDRINKFKKLEYGSLKIGVGDT
                     AARFFLLKYLEIFHKKYPHIHVSTINRTSRELISLLKDGNIDIAIINMPIEDDTLNIV
                     ECIEIHDIFVCANDYIEYKGRKISLEELNTLPLIMLENKANSRLYVNEYFLSKGIKLN
                     PDIELGSHELLLEFAYINLGVSCVIEEFSIDYLENEKLFKLDIKEPIPKRNIGYCHLK
                     DISLSLATKEFLSMISNNI"
     gene            10233..11432
                     /locus_tag="PC414_001131"
     CDS             10233..11432
                     /locus_tag="PC414_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003493023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HDF2463538.1"
                     /translation="MDRLVGTVSRGVRAPIIRQGDDLVKIVVDSVLNASKSENFEVRD
                     KDVIAVTEAVVARAQGNYAHVDNIAKDVKDKFGDDTVGVIFPILSRNRFAICLKGIAK
                     GCRKVVLMLSYPSDEVGNHLISIDELDDKGINPWSDVLTEEKYRELFGYNKHTFTGVD
                     YVDYYKNLIKDCGAEVEIVFANNPKTILNYTTSILTCDIHTRQRTKRILRQNGAKKVY
                     SLDDIMTASVDGSGYNDQYGLLGSNKATEETVKLFPINCDEVVNKIQGNIKEITGKDV
                     EVMVYGDGAFKDPVGKIWELADPVVSPAYTKGLEGTPNEVKLKYLADNDFADLSGDEL
                     KEAISKYIVEKDNKSDDLTGNMVSQGTTPRRLTDLIGSLADLTSGSGDKGTPIIYIQG
                     YFDNYTK"
     gene            complement(11626..14181)
                     /locus_tag="PC414_001132"
     CDS             complement(11626..14181)
                     /locus_tag="PC414_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2463539.1"
                     /translation="MMSLVKFSIQYIKHYKKQSISIVLSIVLSVALLTGIGSLVHSAD
                     KSRIEKIREDSGDYHYFYKVDKEQLNKIKKNKKSKEYTVDRLGVTKPKNQIDEPFIIE
                     FLNADSSYLDMTGRKLLKGTLPSKDGEIVLDTYSLNNLNVNKEIGTEINLDGKIYKLC
                     GILSSLSNPETALEGFVNDYFQPKEKDSYIVYVKYDENKNIKKQNYKFMKKFNISKEK
                     TYINWPLSVEFGVKPPKNIEKYNIFEFLKNLELNTNAIILLVGVFGAAAVYSIFHVSI
                     LQRISQYGVLEVLGANNKQLLFLLFLELFLLFIVGFPIGCFLGIEVASTIYEQFPHIF
                     LSSDIVPGAFFISEKSIYLGFLFILLLLILIAVRVVHQLNKYSSIESMKNFKITNKKK
                     RILESVKHSNMTKVLSHKYMTQKKGMFIGILFSLSLGGIIFLCSSYSIQLTKNNNELT
                     MKADDGLNSDYQITMQTTDFDVGIPKDKISRLNNVEGISKVYPVRYLFGAMQIKEEQL
                     FWKNFFKPLEKDYRIKTFFDGICTKQNEGGYLLKTNIYGYNNGMLEGLNSYIIDGEIN
                     THDMVKNNKVIVRLPMDGTGMYDAVDINPGDTIKVKVPKTMKPTDEIVKFKEENKNDY
                     TTKEFVVAATVKRVMANNIYFIGDYGMDIVMTNGQMKNNFDIENYNSVSIKKTEDSNS
                     EELSENIKSVVSDVKRCIVTDYTIAIEKNNTYLNQKLLFIYGIVFVLLAISLFHIINT
                     VSYLIFSRRHEFGILRAMGITDNKFLLMMIREGFLYGFYASIIMVIGSVIGQFMIYFM
                     VKRVYLYINPILKINTPLYIGMIILNITISIVAVIIPVRQILKSDIISEINKN"
     gene            complement(14178..14864)
                     /locus_tag="PC414_001133"
     CDS             complement(14178..14864)
                     /locus_tag="PC414_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2463540.1"
                     /translation="MSILVTNNLVKHYGKGETKVKALNGVSIEIERDTFTAIVGTSGS
                     GKSTLLNIIGGLDNPTSGDVIIKGKNISKIGKKDLTVFRRRNIGFIFQNYSLMPVLNT
                     YDNIALPVTLDKGNHIDHEYIEMLMKTLGIWDKRLKFPSELSGGQQQRVAIARALANK
                     PALILADEPTGNLDSKTTMEVVCLLKESSAKFHQTILMVTHNENIAQVCDSIIHIEDG
                     VVVNTGGEVL"
     gene            complement(14941..15849)
                     /locus_tag="PC414_001134"
     CDS             complement(14941..15849)
                     /locus_tag="PC414_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2463541.1"
                     /translation="MFILAFIICTVVIFIVSTKINRKRYDELNLMLNQILEGKEVTYP
                     DTKDTRASKISHQVKKIKDMIEIEVEQSKSEKEAIKGLISNMSHQLKTPLSNITIYCE
                     LLENINISTLQKKEFLQKMKNETFKIDWLLQSLFKMTKLEDGVIEFEVEELLIKDTLI
                     QSISTIFNKAEAKNIRINLEPFSDIKLVHNKKWTIEAIVNVLENAIKYSPSDSTITIS
                     VIKMELYTKITIKDEGIGIDSRELNDIFKRFYRSKNVENQNGTGIGLYLTRLILEKEN
                     GNIIVESKLGSGCCFSIFLQNCKSLN"
     gene            complement(15851..16522)
                     /locus_tag="PC414_001135"
     CDS             complement(15851..16522)
                     /locus_tag="PC414_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2463542.1"
                     /translation="MNLLIIEDDINLNEGLFYAFENDGFNVFKAYTKQEGLNIFNSKN
                     IDFIILDCNLPDGDGFDVCQIIREKSDIPIIMLTARDSEIDEVKGLEIGLDDYITKPF
                     SLSVLKARVKVAIRKKSNNKVIYSNGIKLDQKLLKVYKNKECLELSSVEYKLLSYLIE
                     NKGQILLKEQILHHIWDSEENYVDDNIVSVNIRRLRVKVEDDPSNPKYIKTAYGMGYL
                     WNEVE"
     gene            16784..17848
                     /locus_tag="PC414_001136"
     CDS             16784..17848
                     /locus_tag="PC414_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HDF2463543.1"
                     /translation="MKRVDERNTMFARANYKKDSTAYNDYYKKNPDKKEIDDSIRNRP
                     NLCSEGTMTYNELNSPMASSAFDFLSDIKSLCEGKVSDTKVDVDAKSMTKKIKGLAKQ
                     YGASVVGITKLKDYHIYTNRGRHEENYGEEINLTHKYAIVFGCEMDKEMINRAPMICE
                     VIETSKCYVDASIVGMILSYYIRNLGYDARNHMDANYLVMPVFIARDAGLGDIGRNAI
                     LTNKDYGSRLRLGVVTTDIPLLEDEYVDFGLEDFCKVCKKCSFNCPSHSLSNDIKIGD
                     DGKYNWVIEHETCYIKWRYLGTDCGMCISACPFSQNLETIKNTTSFKGNNELIQKALD
                     EYTSKFGKRIFIPGNPSWLK"
     gene            18006..18692
                     /locus_tag="PC414_001137"
     CDS             18006..18692
                     /locus_tag="PC414_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2463544.1"
                     /translation="MEKILVVEDDSILNKTLSYNLIEDGYIITSKFTAKSALKSIFEC
                     EFDLIILDINLPDKSGFELCNEIKENYNIPIIFLTANDMECDMIKGYELGALDYITKP
                     FNINIFKQKIKAFLNHLTIKTKQDYYRDGYLEINFSELSANINGNQIIFTPLEYRTLK
                     LLTENPKSILTRKVLLEKLWDIDANFVDEHTLTSVISRIRSKIEKDNLKYIKTVYGMG
                     YMWLGERNEF"
     gene            18682..19713
                     /locus_tag="PC414_001138"
     CDS             18682..19713
                     /locus_tag="PC414_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2463545.1"
                     /translation="MNFKKFSFSSIYLIIAVGIIFTAILFSISIYLITDDLKLIALLL
                     IYTIVLCGFIALIIFLLHKKIVIFCSRICHTLDSMMNSEDNIEIELEEETILSRINHR
                     LIRMYEVIQENRNSIAIEKADLQELVSDISHQIKTPIANLKMINTTLLNQTFKTEIQK
                     DFLMDMENQLDKLDFLMQSMVKTSRLETGIITLSKKKNSIYETIATALSGILFDAEKK
                     NIEVTVDCDPNLYIIHDKKWTSEAIFNILDNAVKYTSSNGKIRVATECWVMYTKIDII
                     DNGKGISESRQAEIFKRFYREEDVHDIEGIGIGLYLSRKIISLQGGYIKVTSDIGKGS
                     TFSIFLPNK"
     gene            19795..20460
                     /locus_tag="PC414_001139"
     CDS             19795..20460
                     /locus_tag="PC414_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2463546.1"
                     /translation="MSILQVINLKKYYGQSSNITKALDGVTLSIEKGEFISIVGTSGS
                     GKSTLLNMMGGLDNPTSGKVIIKGKELSTMSDEQLTIFRRRNIGFIFQNYNLVPLLNV
                     YENIILPIGLDGETIDKDFINEIINMLSLEEKLYDIPSNLSGGQQQRVAIARALVTKP
                     AIILADEPTGNLDSRTSSDVLSLLKVTSKKFNQTIVMITHNNEIAQLTDRIIRIEDGK
                     IVN"
     gene            20473..22890
                     /locus_tag="PC414_001140"
     CDS             20473..22890
                     /locus_tag="PC414_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2463547.1"
                     /translation="MFRNNNRTFIRKIALNDLKINKLKTYLSGIIIMISTCLLLTVTL
                     VSYNASVDLVNASPYHAIYKSVDEKAKNILYKDKNFSGVGTYKLIGSNKKTDCIMSIV
                     YADDTAIKLMNFQPLKGKLPTNKNEIAISEKYLQEFGLDKDIGDSIKLNYYDDITNKE
                     VQCDFIIVGFLENYYQDNAKQYYTVVSNDYFKNIASTPLQNKSNTFDESRPDTVDVLV
                     KLSEENTEKDSASIKTQLNKIGLSLGIKKYNIYLNDNYIESNLIDGEQIITVLVVGIV
                     VLFSSVFVIYSIFYISVVNSVQMYAKLKSLGMTSFQLKKIISLQGNILSIIFIPLGVI
                     ASCIIAYIIQPLAWQMIADLFIILILSLVMFLTVRISLFKPARIISKISAIEAMQYTE
                     TKFRKKAKNFSYINIKNLALKNIESNRKKNLIALISLSISGVLFISIANLANSIDFKK
                     QLSQQFVYNEDYIISIKSDNLYEHITEIQQNNPLTDSLKNEIKSIHGVKKLIESKSIK
                     SNIIEPYIKDRDEKQFITLIKGITPELSKSLEEYIVSGKIDYTHLDSDSLIINKYRTK
                     YYGLNLKVGDTVTFNIFSGNDIIKKKMKIIGIIDNSNTGGMFFASDKTLESLTPYNTN
                     LDFSILIDKKHSKEVEEKLLSIIQKNSNLMLYSYEDDYRMITRAFQYIIIASYIFVGL
                     ISCFGILNMTNTLINSVLIRKKEFALLQAVGMTRKQLRNMLYREGLNISIKAICTSSI
                     LGYFGSNLLCTFIKDVIRLDFINFKFSIFTILIFSFVLIGIQVLVTELLVRNIEKKSL
                     TERLRSQ"
     gene            complement(23071..23862)
                     /locus_tag="PC414_001141"
     CDS             complement(23071..23862)
                     /locus_tag="PC414_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF2463548.1"
                     /translation="METKQYLIDFYNTYDEDSRLALKHGMVEFLTTMHYIDKYIKSGD
                     CVLEIGAATGRYSHTLARQGYDVDAVELVEHNIEVFHKNTQSNENISITQGNAMDLSV
                     FPDNKYDITLLLGPLYHLYNKEDKQQALHEAIRVTKPGGVVFAAYVISDGCLIDEGFH
                     RGNINVSEYIEKGLIDPQTFAAKSEPKDLFELVRKENIDDLMSAFNVTRLHYVASDGL
                     ALYMREAVDSMDDDAFALYLKYHLATCEREDLVGVTSHAIDIFRK"
     gene            complement(23895..24482)
                     /locus_tag="PC414_001142"
     CDS             complement(23895..24482)
                     /locus_tag="PC414_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2463549.1"
                     /translation="MPPKVKITKEMILNTVLDITREVGFEAVNARSIASKLQCSTRPI
                     FTCYENMNELKNEFLAFAYEYYNQYVVNYRNSKSVSPYLLLPLSYIEFAQEETYLFKL
                     LFINDLDLMMKEANDFYKEIDNEKKARLFSESIGIDLECAKVIFLDLFLYTHGIAVLT
                     ATKKLTLDRYSAEKMLKNILTAFIRQEKSDWNLSI"
     gene            complement(24659..26050)
                     /locus_tag="PC414_001143"
     CDS             complement(24659..26050)
                     /locus_tag="PC414_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HDF2463550.1"
                     /translation="MERLARREQPHIKFGMFKIRIPFIHYRFEKPEAIQGIISSMTSL
                     GTIGLSTQILGLPYEVAWSMAIINSILYCLHVFMGDPVVPGWITASITLTTAYLLKFS
                     MGIERIQALTALQIDLGIIFILMGITGVAGKLVSKIPNSIKGGILMGVGISTIISEFN
                     PKGRFDSYPISITVGILVACFVMFSERFDTLKVKNKFLFHLGEYGVVSAILVSLIVGI
                     FSKEIEIPRFSFDNLVYIVDFKNLINTVSPFGIGFPSVMLFIQGIPMAFMIYIIAFGD
                     FITGENLVLSESENRKDEYIDFNSNRSNVISGIRNIFMAIFSPYIPMCGPLAATLTGS
                     VAQRYKVGKEAMQSVFSGMGTLVWVSAIFICFYPIAQIATPLIPLALSVTLLVQGYLC
                     TKLSMELCNTGVEKGLIGLMGGVIAAKGGTWGLAVGFILYFILIDSKKRKEKDINSIE
                     DCVYEILDKKKSI"
     gene            complement(26234..27538)
                     /locus_tag="PC414_001144"
     CDS             complement(26234..27538)
                     /locus_tag="PC414_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase/BAAT N-terminal
                     domain-containing protein"
                     /protein_id="HDF2463551.1"
                     /translation="MKVNVYNVESFVDERLKIMVSDLIPNTKLKISVKMEFPWCKGEE
                     FSSYGVFCSNEKGEVDLDLVEPIEGTYKADNCMGLIYSLEKSKTEGKNFAENISIDKP
                     IIMNMIFETSIERKEVELKRIFKSEDITTKYISDEFIGKLFYKQNSNNKTILMLGGSD
                     GNLDALDLLAAPLASRGFNVLTVAYFALEGLPDKLEEVPLEYFEKVFEWISENEITST
                     KEIFVHGTSKGGELAFLLASRYEQIKKVVVSQPHIYCFQALNGLMSGNDTSSWSYKGE
                     PFPYIKVDNDIFFEEQKKNISKGIPFGFNSTYKKSLKRAENRKEARIKIENSKADILM
                     IAGKEDNIWNSYEACLKALEIFEENNYPYNVELLTYNNMGHPLPIPYIMPLKETLCMS
                     MSGGIFSSGGTVEGNSKGQFESWNRTIEFYKKPLSSDLNNYY"
     gene            complement(27897..28772)
                     /locus_tag="PC414_001145"
     CDS             complement(27897..28772)
                     /locus_tag="PC414_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HDF2463552.1"
                     /translation="MITFDVNGAIIRFDDTKNNYNKIRKIFKLYALEISKNFERDCLN
                     SFQNLKQISDRGLSLGEKYIEESFKKGIETIVSFGIITIDINTFKSVYCEKYLDFKRL
                     FNNLNKTFIIPNKNKKNGHASFLEIESTIKKMSNYLYNDCFKIHYAVVDALIENRITA
                     VHSYIDEESIKKSNALFNNYKDGFIGKADECKVVKQIITLNPYREDVYEFLVKEDGDF
                     SKEIERLTEFLGYDIREYKASLMDIYINELMESDENDVENAKEKIEKYARYIGCSDEN
                     LYVARVNAIYTFANA"
     gene            complement(28976..29662)
                     /locus_tag="PC414_001146"
     CDS             complement(28976..29662)
                     /locus_tag="PC414_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HDF2463553.1"
                     /translation="MFGVIANSLAIAGGCIVGLIIKGGLPQRVSDTIMNGIALCVLYI
                     GISGALEGKNTLVTIISVAVGALIGELIDIDKWVNKLGAFLQSKFSKGNKKDSIAEGF
                     ISSSLLFCIGAMAVVGSLESGLTGSHDTLFVKSVIDGIASIIFTASLGIGVMFSAISV
                     FLYEGIICVGASFLNGFLSDPVVTEMTAAGSLLIIGLGLNVLKLTNIKVANLLPAIFI
                     PILFGVFGII"
     gene            complement(29711..30244)
                     /locus_tag="PC414_001147"
     CDS             complement(29711..30244)
                     /locus_tag="PC414_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HDF2463554.1"
                     /translation="MSDLNNKKEQDENESSMNETKIYDILDKLNVEYDVVEHEAVYTA
                     EQLVILDGITKGCQCKNLFLRNAKGNKYYLIVVKGEKQVDLNSLKDKIGSSRLSFASP
                     ERLYNVLKLLPGSVNPFSLINDTERKVELYIDKDVIKEDYLNFHPNINTKTVNISRKG
                     FEEFLEYLKYPIQSIDV"
     gene            complement(30288..30920)
                     /locus_tag="PC414_001148"
     CDS             complement(30288..30920)
                     /locus_tag="PC414_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HDF2463555.1"
                     /translation="MKVFDLTHVTHNDMPVYAEPNRPDIKKVAIIEENGYQETLISVF
                     SHNGTHMDSPRHMYTKGETLDKLDIENFVGKAYVLELEKGNENIELEYLKKYEDEIKN
                     SDFIIFKSGWSKFWDKKQYYVGYPTLTKEAANYIANTNIKGIGIDMLSVDRYDTSVFE
                     VHHILFEKGKIIIENLTNLENVPEKFLFIAAPFKYNDADGAPVRAIAIVE"
     gene            complement(31010..32089)
                     /locus_tag="PC414_001149"
     CDS             complement(31010..32089)
                     /locus_tag="PC414_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HDF2463556.1"
                     /translation="MKIAIFLGAGASAAENLPIQNELFSEYFKKLNPTNYNTKMNREL
                     YIFFKQMFNIDIVKDNIDNANFPTFEEVLGLLDLAEQRKEAFRNFGLENLNNRSDSIR
                     FLRQYLILLMAEAIHNTPRTDNKYHKLLVENLLENDLLLDTTFISANYDIHIDNTVTG
                     LRDKNDLPIMLDYGVEFTNFKEELNWRKPKEPLIKLYKIHGSLNWLYCPICNSVTLTP
                     HEGGVMKLIENSSETKCLECGELTEPIIVPPTYFKNMSNIFLSDVWNETEKTLRDTDL
                     LIFCGYSFPEADMHIKYMLKRVQTNRKKPPLKIMVFNNHSQKQRITLKKEEGRYKRFL
                     GEDVIFTDNSFQDFSVNPLRFIKNI"
     gene            complement(32222..33568)
                     /gene="pepV"
                     /locus_tag="PC414_001150"
     CDS             complement(32222..33568)
                     /gene="pepV"
                     /locus_tag="PC414_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HDF2463557.1"
                     /translation="MKEQIKEKVNSLQDEMISSIQESVKIPSVISEATENCPFGENVD
                     KALRGILDLCKSLGFKTVYKDGYYGYAEIGQGEKMIGILGHVDVVPEGDLESWNYPPF
                     EAVLEDGKLYGRGTQDDKGPTISAIYAVKALMDLNVDFNKRIRFIFGADEENLWRCIN
                     KYKENNEEIPNYGFTPDSRFPITNAEKGLLQVHLTCDSKSDIELSVGKALNAVPGKAI
                     YIGKYSDKLKKELNKLNFEYTVEGNKICIIGKSVHSAASDTGINAVARLCIALNNIGI
                     DSNIIKFLAEVIGEDANGNNIIPNCKDDVSGKLTVNIGRVTIDNEKEFAGIDVRIPVT
                     YKKDDFVKELKKMTDKYNLNYEEYDFLDSIYVPEDTLLVKTLRKVYEEETGLDGTPLS
                     SGGATYARALDNCVAFGAIFPGKPETEHQANEYLIVEDIIKATQIYALSIYELLKI"
     gene            complement(33637..34917)
                     /locus_tag="PC414_001151"
     CDS             complement(33637..34917)
                     /locus_tag="PC414_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HDF2463558.1"
                     /translation="MLNETKELLKDYYGIDDDTFKLSQEIMEEIKDKFEEIKEIREYN
                     QYKVLKAMQESKLSDMHFNWTTGYGYNDIGREKIEEIYSKVFNTEDALVRPIIVNGTH
                     ALTLCIQGIVRPGDEILSVTGRPYDTLEGVIGIREEKGSLKEYGVTYDDVDFLEDGNL
                     DLEGIKNKINDRTKLVMIQRSKGYSWRKSLSISDIKEAIEVIKSVKPEAIVMVDNCYG
                     EFLDTKEPTDVGADVMAGSLIKNPGGGLALTGGYIAGRKDLIELISYRMTSPGIGKEC
                     GLTFGTTRNVLQGFFLAPYIVSQAVMGAIFCSRAFEKLGYDVLPKYDDLRSDIIQCIR
                     LNNADEVISFCEGIQEAAPVDSYVKPVPWDMPGYESEVIMAAGAFIQGSSIELSADAP
                     IRPPYNVYFQGGLTFDHSKMGTLKAIEFIKKLKK"
     gene            complement(35044..35304)
                     /gene="hfq"
                     /locus_tag="PC414_001152"
     CDS             complement(35044..35304)
                     /gene="hfq"
                     /locus_tag="PC414_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HDF2463559.1"
                     /translation="MKNTVLNLQDLFLNNARKERIPVTIYLVNGVQVKGLVKGFDSYI
                     ILIEGDNRQQNMIYKHAVSTIQPGKYINLTNQNQNNNNNNNR"
     gene            complement(35418..36353)
                     /gene="miaA"
                     /locus_tag="PC414_001153"
     CDS             complement(35418..36353)
                     /gene="miaA"
                     /locus_tag="PC414_001153"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDF2463560.1"
                     /translation="MKKIPLIILTGPTAVGKTDLSIKLAKDMNAEIISADSMQIYEYM
                     DIGSAKVTEEEMQGVKHYLIDEVKPDYPFSVSEFQQRAKKYIHEINKKEKCVLVTGGT
                     GLYLNSLIYNMDFAQSDANNELREELQKQLAEKGIDYMHNKLKELDEESANRIHKNNT
                     KRVIRALEVCLSGKKMNDFSSDLKFNEEYQPIIIVLNRDREHLYQRINMRVDIMLKNG
                     LVEEVKKLLSMGFKKDMISMQGIGYKEILKYLDGEYTYEEAIEIIKRDSRRYAKRQIT
                     WFKRYKTAKWFDLDQYENIDELKNEIILYIKDSIK"
     gene            complement(36391..38358)
                     /gene="mutL"
                     /locus_tag="PC414_001154"
     CDS             complement(36391..38358)
                     /gene="mutL"
                     /locus_tag="PC414_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDF2463561.1"
                     /translation="MKNIINILDDLTINKIAAGEVVERPSSVVKELIENSIDAGANKI
                     SIDIIDGGKSLIKITDNGIGIPSSEVEKSFLRHATSKIKKIDDLYDLYSLGFRGEALA
                     SISAVSKLEMTTKTKDEIIGTKIYVEGGKIISKEPIGSTNGTTIIIKDIFFNTPARQK
                     FLKSTHAETINISDLINKLAIGNPNIQFKYTNNNKQMLNTPGDGKLVNTIRSIYGKEI
                     TENIIDVEFKCNHFKMNGYIGNNNIYRSNKNLQHIYINKRFVKSKIIIDAITESYKSI
                     IPIGKHAVCFLNIEVDPSCIDVNIHPNKLEIKFEKEQEVYIELRDFLKVKLIHSNLIG
                     KYATYSDKKTQPRIAINSREKSTDYKLRNNDLLESTPKNSNITKGKDEVIEVVTLSSE
                     KPINEFQSVSEVLNASVEDDVKNINYLSEDSANDNIQEEFQVDGIKNEGNYYLGDSIK
                     DSEEEYLCSSKRKFSLYGYSVIGVVFNTYIILSKDDSMYLLDQHAAHERILYERYMEK
                     FYRQDINMQILLDPVVIEVSNVDMLQIENNLELFMKFGFELEIFGNNHIMVRCVPTIF
                     GVPETEKFILQIIDNIEEITSNYDLKGERFASMACRSAIKANDKIYDIEIKSLLEQLE
                     KCENPFTCPHGRPIMVEISKTEIEKMFKRIM"
     gene            complement(38369..41212)
                     /gene="mutS"
                     /locus_tag="PC414_001155"
     CDS             complement(38369..41212)
                     /gene="mutS"
                     /locus_tag="PC414_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDF2463562.1"
                     /translation="MQIDMNKLTPMMKQYLEVKNRYKDCILFFRLGDFYEMFFEDALV
                     ASKALEIALTGKACGLEERAPMCGVPFHSANSYISKLVENGYKVAIGEQMEDPSTAKG
                     IVRREVIRVITPGTVLDGNLLENKKNNYLLSLYKDGTNIGLTYVDISTGETNATCLNE
                     DKVIEEIAKIHPTEIIINDLDFIENLRDIATVSNIYINESFSDNYLDINILKEYFPDV
                     YLQKLKFDDKGLIKSSLSILLNYIYNTQKQITSNINNINIYNSSEYMVLDMFTRTNLE
                     LTQTIRGNKKKGSLLHVLDKTSTAMGGRLLRKYVEEPLINKSKIENRLDVIEEIKDDF
                     ILREDLNDILKNIYDIERICGKIAFERVTPKELIHLKNSIEKLPNLKDTINLSNAKIL
                     KEYVSEMDKLDDIYNLIDEAILEEPTITIKDGNIIKSDFSDELKELREISKNGAFLVK
                     EIENREREKTGVKSLKIGFNKVFGYYIEITKANFKQAKLDETYIRKQTLSNAERYITP
                     ELKEIEEKILHAEEKIKSLEYEIFVEIRDTIYKNIDRIQKVAKTIANIDVFVSLATVA
                     HINNYVKPAINENNKLDIRNGRHPVVENIVGEENFVPNDTYLNRGENIINIITGPNMS
                     GKSTYMRQTAIIALMAHIGSFVPAESADIPILDRIFTRVGASDDLSQGQSTFMVEMNE
                     VSLILKNATERSLVILDEIGRGTSTYDGISLAWSIVEYIQKNIRCKTLFATHYHELTD
                     LEEEFKEVKNYSIAVKEDGEGIIFLRKIIPQGADKSYGIYVAKLAKLPDEVIERAKYI
                     LKDLEKNHVYNSVAINGDKENNNIINSNLDEELVKVNQSNFKNKYESLKIEHELIVKD
                     YKHIKKDYDKLNSKFKALNDEVALIKQNDDKEKINQVDSVKEVALTQISFDSVNRDIL
                     SEEILNLDILNMTPLDAINSLYNLQRKAKEIKS"
     gene            complement(41372..42106)
                     /locus_tag="PC414_001156"
     CDS             complement(41372..42106)
                     /locus_tag="PC414_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2463563.1"
                     /translation="MLTQMNFYHNKKVKVSIENVSKTYKDIEVLKDISIDVYEGEFVS
                     ILGPSGCGKSTIFNIITKLTDYDSGKVNINGKYSYMYQKDLLLPYKTIIDNVSLPLIL
                     KKEKKSKARSMVKPYFEVFGLSGYEDKYPSELSGGMRQRANFLRTFINSNDIMLLDEP
                     FGALDSLTKTSMQKWLLDVKKKVNSTILLITHDIDEAIMLSNRIYVISKKPSIVKKEF
                     VIDSRKLNEDNLENIIKLKKEIISLL"
     gene            complement(42075..43088)
                     /locus_tag="PC414_001157"
     CDS             complement(42075..43088)
                     /locus_tag="PC414_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2463564.1"
                     /translation="MNFKKLGILGLVSVFTLATITGCSSNNNKDKNNDKDTSSKKVTM
                     VLDWTPNTNHTGLFVALDKGYYKEEGLDVEIVQPPESGAETLVATGKADFGISYQEQV
                     TYAKTSEDPLPIKAVATVIQHNTSGFASPKEKNITTAKDFEGKTYGGWGSPSEEAVFK
                     AVMKKNGADFNKLKIVNTGQDDFFAAMKTVDFAWIFEGWDAVKADLIGYDLNFIPVKD
                     LDERLDYYTPLIISNETVLKDNPELAKKFLKATTKGYEYAIKNPEESAKILVKHAPEV
                     DEKLALKSQEYLASKYKDDAPRWGEMKDSVWNNYTSFLKEYKLIDKDMKASDAYTNEF
                     LPQ"
     gene            complement(43110..43880)
                     /locus_tag="PC414_001158"
     CDS             complement(43110..43880)
                     /locus_tag="PC414_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2463565.1"
                     /translation="MKNLEIIDKVKDSIYPIATFLFILILWQSMVGVLEVPQYILPTP
                     VDIINVFFKDYQNLSMHAVVTIGEAILGFIVAIIISLVIGILMDFVSIIKKCLYPIML
                     VTQMIPTIIIAPLFMIWFGFGTMPKVLMVMLTCFFPILISFVDGIENIDKDYLNLFKT
                     MDSNKVNTFIHLKFPMAMDKFFSGLKISATYAVMAATVAEWLGGTKGLGVYMLRSKSA
                     YALDKVFASTILVVIFSLMFVGIVQAVKKVVIRHRLID"
     gene            complement(43855..44145)
                     /locus_tag="PC414_001159"
     CDS             complement(43855..44145)
                     /locus_tag="PC414_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HDF2463566.1"
                     /translation="MVIADIAVMPLRPYKGEEEMYKVVDACIAEIEKSGLKCEIGAMS
                     TTVEGDFDEVFELLKKVHKIPFNLGCERVITVARVDEKAGGLTIDEKLRNHR"
     regulatory      complement(44276..44375)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     regulatory      44995..45084
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            45137..45313
                     /locus_tag="PC414_001160"
     CDS             45137..45313
                     /locus_tag="PC414_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463567.1"
                     /translation="MFMDKMDRCTHILTAYICSSRDYCNFIDTQLNDFILEYGENVVE
                     SCLHQVMVLVSRYN"
     gene            complement(45428..45784)
                     /locus_tag="PC414_001161"
     CDS             complement(45428..45784)
                     /locus_tag="PC414_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2463568.1"
                     /translation="MDIDNNFEEFPNIEERQKDFNCSIGFTMTVIGSKWRAIILWHIL
                     KQEPIRYSQLKKEVVHISHKILSQELKYLERDGLIKRISYPTIPPKVEYLSTKRGKSL
                     ESILLELCNWGKQYMR"
     gene            46251..46999
                     /locus_tag="PC414_001162"
                     /pseudo
     CDS             46251..46999
                     /locus_tag="PC414_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816229.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
BASE COUNT        16131 a         7314 c         5413 g        18248 t
ORIGIN      
        1 ccaattcata tatgcaaaat ttatatactt taaaatatct aagaaattat tcaataaaaa
       61 aatactattt tttaaaaatt atcattattg ataaagctct actatttata ttttttagtg
      121 tattcatgta taataagtaa aagcacaacg gtatatactg ttgtgctttt ttgtaattaa
      181 taaacaatat tttggtctat aacaatgaaa aatggctact ttccataatt gatttcatct
      241 gacataagca aaaagggtgt ccagctgggt caatcataac tctccagtca tcagtaaatt
      301 gctcctctgc gattgtagct ccacaatgaa ttgcatattg aactgcttct tctaaatcct
      361 taacagcgaa atccatatga gccatttgct gttgagattc aggtttttct ggttctatta
      421 tgccccaagc ctttgttttt tgcaatataa agaaaaaatt atatatttta atattttata
      481 cttgttgaaa ttgtgtaata aattcaactg gatacatagt gcgttttgaa atttcttcta
      541 aagctaaacc ttgttttaaa agcctcttta tgacgctttc catactttct gaaatttcta
      601 taatattttt atcaaaatca taaaacctaa aagttctttg accccatggg tattctttta
      661 tataatgcaa aaattcaata tttcctattc tatttatctt actttcccat tgtttgatgt
      721 cattaacctc aaaatacaac tgaaagttat ttgattgttg atatattggt aaatcatatc
      781 caattatagc tttataatta ctttgtaatc caaatccatt ttcaaatgat acatgttctc
      841 ctaaatctaa tgtgattttt tgctccataa catcttcaaa aaactttttt gatttattta
      901 tgtctttgac aattagcaat actcctccaa atttcatctt atcactatcc tttctttgtt
      961 ataagtatta taacttaaaa ttttgcaatt tcactgtaaa aatcagacat attagctcgg
     1021 tatttttgag gagttattcc acagacaatt ttaaaatctt taataaaatg tggtatatca
     1081 tagaatccta attcattaca tatagacatc catgaagttt tattattgtt tagaagtttt
     1141 atagatttat taatacgtac caaacgagaa aatgacttca tactcaaccc tagatactga
     1201 ttaaagagtc tgtttaaatg cctagaacta taaaatactt ttttagaaat ttcttgtgaa
     1261 gatatatttc cagagttttg aataatcaac tgaattgcca aaataaactc atatgagtat
     1321 tcaattttaa tattagaaat caataattct tccaacttta aaatcaattt atctatagat
     1381 aaagattcat taaatatttt tttaatttct aaatctaatg aagtattaat tatagaaaat
     1441 ggaacaactt tatcaataag ttcactttgc tgtataccta taagcgggaa aaaaccagca
     1501 ggttgaaatt ctataattag tataacatca catctattag caatatttcc tactgttttt
     1561 ggtttagtag ttggaccaaa taatagacta tgtaaccctg tggcatcata aaaaaacacc
     1621 aaagttacac ttccatgagg aataatcgtg taattatcag aaattattgt ttgatttgga
     1681 aacgatatag taaaattcga tatccaattt cttaattgta tacaaggcaa gagataaaca
     1741 aaattgtctt cacaaactat aagtgaccta tttttattta taaaatgaat actttctttt
     1801 tttaacatat attatatcct gtcctatcgt gaatatttat taaagtaaga agaatccata
     1861 ttatctctat atatcattaa ctgcttttaa taatagagat agtatgaata ctcattagat
     1921 atttaataaa caaataaaat taaaataatc aataaaaaat atagtacttt tatctgttat
     1981 tttaatatta tagcacattt atagtattta acttatatcc actttacata tgtctaattt
     2041 atataattaa aaatgcttat ctcaaataca ataattagtc cttatacaat taaaccatct
     2101 gaaactgtaa tagtacgact actacttttg gctgcttctg gtgagtgagt taccataatt
     2161 atagtttgtc cattgtctct gtttatttta ttcagtaaat ctattatttc tatccctgtt
     2221 ttactatcta aatttccagt aggctcatca gcaaatagaa tctctggttt tccaattaaa
     2281 gctcgagcta tagcaacacg ttgttgctga ccaccagata actctcgtgg tgtatgttta
     2341 cgtctatcag ttaaaccaac tatatctaag atttcatcta attgattttt ataatctttt
     2401 aaatttttac catctaataa caaaggaagc attatatttt cctccacatt taaatttggt
     2461 attaagttat aaaattgaaa tacgaatcct atatttctac gacgtattat gctcattttc
     2521 tcatcattaa aatgtgatat atcagctcca ttcatataca cttttccgct cgttggagta
     2581 tctaaaccac ctaaaatata taaaagagta cttttaccag aaccagattg acccatgata
     2641 gaaacaaatt cacctttcat tacttgtaaa gatacctctt ttaacactcg agttgttgta
     2701 tttccaattt tatattcttt gataatattt tttgcttcta cagctaaact attatgtgta
     2761 attttcatat taacctccat aattattcaa acttaatttc ttctatcagt ttcattttct
     2821 tcccttttat aataggcact atcgaaccta ttaaagttac tataattccc aataacccaa
     2881 caataataaa tgttttaaaa tctaaatctg gtttcattga aatttttggt cctgcaacta
     2941 taaagatagt ttgaatctca agtatagata tcacaatacc aataacagct ccaagtaaac
     3001 ctgaagtaac cccttcaatc aatgttactt ttatattttg tttattactt agtcctatag
     3061 atttatacat agctatagaa cgtctttttt gaatataatt aatcagtaga ttattaataa
     3121 tacctactgt agcaagaaga aaaatgaaat aggtcatctt attcatagga gacaaaaaac
     3181 tacttatagt atttaaagaa tcattattaa attcttcaac tgtacgactc caattatatg
     3241 tatctccaaa taaatatctt atctgaatca taacagcatc tggattgact gctgtatata
     3301 ctaaaaaacc ataattagtt ttatcaaaat cattaactgc ataatgtgat ggtataacag
     3361 cttctacatc atttgcacgg gatttaaaac tacctactat tttataagaa aaatcatact
     3421 tatcattaga tagagttatt gtatctccta tagataagcc tacttttttt agagtatctt
     3481 cattcaaaat aacagaacgt ttatcattaa aagcttctat tacttgtttt tttatctcaa
     3541 aatttgtata attgattcca aacatagagt tatatagttt tatattatct gtaccctcta
     3601 gtcttgataa tgttacacca ttaccagaaa tctcattatt cataacataa agaggtaata
     3661 tttttttaat tccttccata tgtctaacat cttcaataaa ctcttcattc atatttccat
     3721 ctgcaaaacc ttgtaattct gcgtcacgaa acacatctgt tatataagtt ttgacaaagt
     3781 taccaacaac acttattgca ataactgcag aaatacttat aaatagtaaa gtgatattct
     3841 gtattatatt tttgttattt ttcatatttc tagcagctaa tttaccctca ttccctaaaa
     3901 tatttttata aacaaactca aaaactatag acatgatatc agtaataaga ggaatcaaca
     3961 caatagtagc tacaattaat cctagtaatg aaaatccacc agctagatac aatgtatttt
     4021 caggggatat tcttggaagt aaaatagata aaataaacat tatacttcct ataaaaagta
     4081 tagaacgatt tgaaacattc ttttcttcta ctgttcctag tacaatattt tttatcggca
     4141 aatggctagc ttttttaaca ggtatatatg cactaaatga agaaacgatt attgctacta
     4201 taacagatat aatcactcca atgggtgata ttactactgg aattgatatt ccttgttcga
     4261 gagaatttcc taacccatga agcattaaat ttaaaacaac tacaccaata ggaatagcaa
     4321 ttaatcctcc tatacttccg tataatatac tttcaagcat caatatacga gttacagttt
     4381 tttcattggc accaatactt ctaaatgttc caataaaagg aagacgttct aatgtaataa
     4441 ccttataact gctgtatatt ataaatatac taagggttaa tgcaaaaaaa cttattaaaa
     4501 agaaaggcat tgtcttttgt ctagcatctg aaattatctt agtttcattt atagtatttg
     4561 ataccatata tttttcagta gaaagttctt cggataattt atttactaga ttttctgtaa
     4621 tttcttcttc tgactctata agaactcttg tatatccact gcctttattt ataatttttg
     4681 atagagtctc tttgggtagc agtgcattta cacctcttgt gttcctcaaa aatactgtat
     4741 cataagatgc aatatcacta acttgaaaag tataagactt tccataaatc tgcaaagtaa
     4801 tagaatcacc tttttttatt ttatattttg atgtaaagcg atttggaaga ataattttat
     4861 caccagaaaa atcagtaata ctatcgccat tttctaatcg tggcttgttg attttattta
     4921 gttgacttaa gtctgctgat ataagactaa attcttcata gtatccccct ttattataaa
     4981 tagcagagct ttccaataca ccaacctttg attttatagt atttaaatca ggtatgtctt
     5041 ctaaattggt taatatatct ggatttttac tttggacaga tatagtcgct gtaccataca
     5101 taccttttgc catttttctt tgtgcacttt cataagataa tccaattgag aatgaaacaa
     5161 aaagtaaaac tgttgataaa actatggaca ataacataac tgctgttcta atttttcgtt
     5221 ctttaatatt tgtgaaaata tactttaata taattttcaa acttacacct tcttccctta
     5281 tctaagatat ttcaatctaa tatttttata ctttaatcta aattaaatct ctaatagact
     5341 cctccatact tttatcattc tatttataat atttttaatc aaatcacccc ttaaataaaa
     5401 aagattgata aactactata taaatataat aatctatcaa ccttaatata ttttttattt
     5461 aaccttaatt aaccttaatt aaataggaaa ggtgatataa aatgagctac ctaatccagg
     5521 tatactttca acagatatat tccctccatg aacttcaata atttgcttcg ttattgctag
     5581 tcctaaacca gttccttcta ttttttgttt tgtattgctt cctctataat aacgattaaa
     5641 caaatttgat gcttcttctc ttgacatccc ttttccatta tcagatataa tgatttcaat
     5701 tctttcattt aatgttacat gtacacaaac tttagtattt ttatctccat atgtaaaagc
     5761 attgacaata agattactaa aagctcgttt taatagtgta caatcaaaat caaagttaat
     5821 attatctata ttactcttaa attcaactat atttgattca tattctggat aatttaaaat
     5881 atcaatggat aattctttca agaatcttac taaatcataa ttatttttat ttaaagggat
     5941 tattccattt tttaattgat aggttaattt taaatcgttt ataagtgtat ttgtatattc
     6001 tatgttcttt aacatgattg aagcatattt atatactttt tcaggtgaat aattagtact
     6061 actatctgta agaagctctg catacccttt tattggtgaa agtggagttt ttaaatcatg
     6121 agttatattt gaaatccact cttctctcat aatatcagtt ttattttgta tctcatcact
     6181 tttatgaatt tcttcattta aaatatttaa actattatat atatcagcaa aaacaccttt
     6241 aatattaact ggaagatagc ttcgttttga aatatcacta acagcagatg taatatttga
     6301 tatgcacttt gttacccagt aaccataagc aattccagaa accaaaatta ttaaaaatat
     6361 aatccctatg attgaaaata caattgactt accacctata aatttacctc catcaaaaaa
     6421 catattcact ttagatatat ttaatggaaa atatataata taaatatagt catttttatt
     6481 atttttaatt gtattaaagt atacagttcc attacttgaa ttattttggt tagctaatgc
     6541 taataattca gaattagaat aataatcttt atgctctttt ggttctaaaa acccataaac
     6601 tctatttcca ctagaatcta aaatttgcaa ccacaattga ttttctcgaa gtttttctaa
     6661 tccagactgt tttacttgtg gcttattatc tataaatacg atttgttcat taaagttttc
     6721 tgtaaatttt ttcggccagt cactacgaat aaattcccca tttggtttct gaatagtaat
     6781 aagtgataag aaaataccta ttactgtcag gattaagcaa ataaaaatca tgaaaaaaat
     6841 aatgtatgaa tgaaatactg ttttataact aggtttatgc atttaatcaa ctcttttctt
     6901 aaacttttat tttagtttga ttgttgctta tataaaatag acaataatta ttgatatgta
     6961 aaaatttatt taaaaataca actatctaca taaattatat ttatataatt acatgttatc
     7021 aagctgtctg ttgtatttaa taaatcattt tgataacata taaaagtaag ataaattttt
     7081 ccattaaata ccttataatt atacacaaaa tctctatata cgttaaacaa tatagagaaa
     7141 attttcatag aaccatcttt ataggggata ctttataaaa gaataaaaaa attataatat
     7201 ataaaaaact tcataggagt gtaacaacat cagtaaagat atgaaaacac tatataaaaa
     7261 ttcaaaaata ttaagaataa tgaaatactt gaaattgctt atatcaagaa attttatata
     7321 atataaactg gtcaaatatg ttataatatt aaattgtgac aagttcatgg aggtgtttat
     7381 atgaaaaaac aaaagtcgcc aattttactt gaaatgcttg gactattttt tggttgtata
     7441 tcaatgagta ttggtataaa tatgttttta aaaccacata caattgctcc tggaggactt
     7501 agtggtctat cattagtact aaacaaggtt acaggacttc cagtatcagc tatcatgtta
     7561 attataggtg tcccattagt aatacttgct tttagaatta tgggtacaaa aaattcatta
     7621 aaaactttat ttggaacagt tgtattttca gctatagttc aattaacaga cccattatca
     7681 aaactaagat ttactaatga cttattattg tcttctattg caggaggaat cctagttggt
     7741 ataggtttag gaataatgtt taaatcagat gcttctactg gaggaacaga cttaattgca
     7801 ctcatactta gtaagaaatt cccaggaata aaagctacaa aattcatgtc atgcctagat
     7861 ggaatggttg ttatatcttc tggtatagta aatcgtagtt tagaaactgc tctttactct
     7921 ggtatagcac tttgtgtatt aataaaaata gcagatatga taatggaagg atttgaccat
     7981 tcaaaagcat tttttattat ctctgatgag cctgaaagtt taagacaagc tataactgaa
     8041 gaacttgata gaggtcttac tattcttgat ggtaaaggtg gatacactag agaaaataaa
     8101 gaagttctat tagtcgtagt ttctaaaaaa caagaacttt atctaaagag attagttaaa
     8161 agaactgacc ctactgcatt tgttattgtt acagatgtac atgaggttct tggagaagga
     8221 tttaaaaatt tagagaacta ataaaaatta atattttaag tatatatact attttaatta
     8281 aaatacgatt tattcaaaaa gagtgtctca taaacttttt agttcatgag acactctttt
     8341 ttgtataaaa tcaaatgcat tttcagtatt ttaacaataa aaacaggatt atctatattt
     8401 tgaggcaacc actttattga aattttattt agaaaacaat cattactaaa taaccagcat
     8461 agatattaaa tgattatatc tattgttttt tcaattcata ttgataacaa ggaacatttt
     8521 ttacagtatt tccagtatga atttttttga tatactttcc tgtacattta aatcctaata
     8581 aatctaatga ttttgtagaa ttttggtttt cttcataggt tattgcaatt attttattta
     8641 agtttagttc cctaaatcca tactgtatta ctctttttgt tatctcagta gcaaaatcat
     8701 gtccccaata gcttggattt atacatatac ctatttctgc ttctaatgct ttaggattta
     8761 tattattaaa ccccatattc ccaataactt tattgtctct tttatatact atagcaaagt
     8821 ggccaatttt ccctttttta tatggattta gaaaaaatga ttttataaac tttcttgtat
     8881 cttctaaaga tttatgctca ctaaatggta aatgttttac tactatttta tttttataac
     8941 agtcaaaaaa atcttcaaca tcatctaatg atgcaggtct tagtatacaa cttctagttt
     9001 ctaaggtttt catttgagga tattcagaat cagacatttc taatccccct ttatacatta
     9061 atctaaatat actgttcata tttaaaatac gatattaatt atataaatgt tctcaaatta
     9121 accttatttt tatttttaat aaaaactaat tactatatga atctttcaaa tttacgaatt
     9181 tatctctatt tttatattaa attacttgtg aaatatgtat tctaaattat atattattag
     9241 atatcatgct taaaaattct ttggttgcta aggataaaga tatatctttt aaatggcaat
     9301 aacctatatt tctcttaggt attggctctt ttatatctaa tttaaacaat ttctcatttt
     9361 ccaaataatc tatgctaaat tcttctataa cacaagacac tccaagattt atatacgcaa
     9421 attctaaaag taattcatga gaacccaatt ctatatctgg gtttagtttt ataccttttg
     9481 ataaaaaata ttcatttaca taaagtcttg aatttgcttt attttctagc attattaaag
     9541 gcaatgtgtt taattcttcc aatgatattt tcctaccttt gtattctatg taatcatttg
     9601 cacatacaaa tatatcatgt atttcaatac actcaacaat atttagtgta tcatcttcta
     9661 ttggcatgtt tataattgct atatctatat tcccatcttt gagtaaagat ataagttctc
     9721 tactcgttct atttattgta gatacatgta tatgtggata cttcttatga aaaatttcta
     9781 aatattttaa caagaaaaaa cgtgcagctg tatctcctac tcctattttc agagaaccgt
     9841 attctaattt tttaaattta tttattctat cttctcctga tgatattaac tttaatgcag
     9901 tagtgacatg ttcacttaga attttcccct ctggagttaa ttttactcct ttattgtttc
     9961 tataaaataa taaagtatct aattgttctt ctaattgctt tattgactgg cttacagctg
    10021 gctgtgacat aaacaattcc ttagcagcaa gagaaaaact cttattattt acaactgcat
    10081 taaaaacctt ataaagttca agttttatat tcatatataa ttactcctta tgtcaaatat
    10141 tagtattatt tattttactt ataattatat ttgaattata atatacatgg tactaatatg
    10201 acaatattaa ttctttagga ggattttaga taatggatag attagttgga actgtttcaa
    10261 gaggggttag agcacctatt ataagacaag gagatgactt agttaaaata gttgttgatt
    10321 ctgttttaaa tgcgtcaaaa agtgaaaact ttgaagttag ggacaaagat gtaattgccg
    10381 ttacagaagc tgttgtagct agagcacagg gaaattatgc tcacgttgac aatattgcaa
    10441 aagatgtaaa agataaattt ggagatgata ctgttggagt tatattccca attttaagta
    10501 gaaatcgttt tgctatatgt ttaaaaggaa ttgcaaaagg atgtagaaaa gtcgtattaa
    10561 tgttaagcta cccttctgat gaagttggaa atcacttaat aagtattgat gaacttgatg
    10621 ataagggaat aaatccttgg tcagatgttt taacagaaga aaaatacaga gaactatttg
    10681 gatataacaa gcatacattt actggtgtag attatgttga ttattataag aatttaatca
    10741 aggattgtgg tgcagaggta gaaatagtat ttgcaaacaa tccaaaaact attcttaact
    10801 atacaacaag tattttaact tgtgatatac atacaagaca aagaacaaag agaattttaa
    10861 gacaaaatgg agctaaaaaa gtatattcac ttgatgatat aatgacagca agtgtagatg
    10921 gtagtggtta caatgaccag tatggacttt taggttcaaa caaagctaca gaagaaacag
    10981 taaaattatt ccctataaat tgtgatgaag ttgtaaacaa aatccaaggt aatataaaag
    11041 aaataacagg gaaagatgta gaagtaatgg tttatggaga tggagctttt aaagaccctg
    11101 taggtaaaat atgggaatta gctgacccag tagtatctcc tgcttataca aaagggctag
    11161 agggaactcc aaacgaagta aaattaaaat acttagcaga taatgatttt gctgacttat
    11221 ctggtgatga gttaaaagaa gcaatttcaa aatacatagt tgaaaaagat aataaatctg
    11281 atgatttaac aggaaacatg gtttctcaag gtactactcc aagaagatta acagacctta
    11341 taggttcttt agcagattta acatctggaa gtggagataa gggtactcct attatttaca
    11401 tacaaggata tttcgataac tatacaaaat agtatgtttt aataagattt aatttttttg
    11461 gattatatat attaacatga aaatttatta aaacctctta atagatatga cttttcaaga
    11521 gttatatcta ttaagaggtt tatttaatgt tcacagataa gatatattta aataatttaa
    11581 acaagattgt cagaaagtta tataatctta aaaatttata taactctaat tcttatttat
    11641 ttctgaaata atatcactct tcaaaatttg tcttactgga ataattacag caactatact
    11701 aatagtgata tttaaaataa tcatacctat gtatagaggt gtatttattt taagaattgg
    11761 atttatatat aaatatacac gttttaccat aaaataaatc ataaattgtc caataacact
    11821 tccaattacc attataatac ttgcatagaa tccatataaa aaaccttctc ttatcatcat
    11881 aagtaaaaac ttattatccg taatacccat agctcttaaa attccaaact catgtcttct
    11941 tgaaaaaatt aagtagctta ctgtattgat tatatggaac aaacttattg caagaagtac
    12001 aaatacaata ccatatataa ataagagttt ttgatttaag taggtattat ttttttcaat
    12061 agcaatggta taatctgtta cgatacatct ttttacatca cttacaacac tttttatgtt
    12121 ctcagacaat tcctcactgt tactatcttc tgtctttttt atacttactg aattataatt
    12181 ttcgatatca aagttatttt tcatttgccc attcgtcata actatatcca taccataatc
    12241 accaatgaaa tatatgttat ttgccatcac acgttttaca gtagcagcaa ctacaaattc
    12301 ttttgttgta taatcattct tattttcttc tttaaattta acaatttcat ctgttggttt
    12361 cattgtcttt ggcactttaa cttttattgt atctcctgga tttatatcaa ctgcatcata
    12421 catcccagtt ccatccatag gtagtctaac tataacctta ttatttttaa ccatatcatg
    12481 ggtattaatc tccccatcta taatataaga atttaatcct tctaacatac cattgttata
    12541 accataaata tttgttttta aaagatagcc accttcattt tgctttgtac aaataccatc
    12601 aaaaaaagtc tttatcctgt aatctttttc aagaggttta aagaaatttt tccaaaatag
    12661 ttgttcttct ttaatttgca tagctccaaa taaatatctt actggataga cttttgatat
    12721 tccctcaaca ttgtttaatc ttgaaatttt atcttttggt atacctacat caaaatctgt
    12781 tgtttgcata gttatttggt agtcagaatt taaaccatca tcagctttca ttgtaagttc
    12841 attattattt ttagtaagct gtatagagta actagaacat aaaaatataa ttccaccaag
    12901 tgataatgaa aacaaaattc caataaacat tccctttttt tgagtcatgt atttatgaga
    12961 taatactttt gtcatgttag agtgttttac actttctaat attctctttt tcttattagt
    13021 aatcttaaag tttttcatag attctatact agagtattta ttcaactgat gtacaacacg
    13081 tacagcaatt aaaattaaca acaataagat aaataaaaag cctaaataaa tagatttttc
    13141 acttataaaa aatgctcctg gaacaatatc actactcaaa aatatatgtg gaaattgttc
    13201 atatattgta gacgccactt caatacctaa gaagcaacct ataggaaatc caacaataaa
    13261 aagtaaaaat aactctaaaa atagtagaaa taaaagttgt ttattattag cgcctaatac
    13321 ttctaaaacc ccatattgtg atattctctg aagtattgaa acatggaaaa tactatatac
    13381 tgctgctgca ccaaacactc caacaagtaa tataattgca tttgtattta gttctaaatt
    13441 ttttaagaac tcaaatatat tatatttttc tatatttttt ggtggtttta ctccaaattc
    13501 aactgaaaga ggccagttta tataagtttt ttcttttgat atgttgaatt ttttcataaa
    13561 tttataattt tgctttttaa tatttttatt ttcatcatat tttacataaa ctatataaga
    13621 atccttttct ttaggctgaa aataatcgtt tacaaatccc tccagagcag tttctgggtt
    13681 tgaaagactt gataaaatac cacataactt atagatttta ccatctaaat tgatttctgt
    13741 tccaatttct ttgtttacat ttaaattgtt tagagaatat gtgtcaagta ctatctctcc
    13801 atctttacta ggaagagttc cttttaataa ttttctacct gtcatgtcta aatatgaaga
    13861 atcagcattt aaaaattcta taataaaagg ttcatctatc tgatttttag gttttgtaac
    13921 gccaagtcta tctacagtgt attcctttga ttttttattt tttttaattt tattaagttg
    13981 ttccttatct actttataga aataatgata atctccacta tcttccctta tcttttcaat
    14041 tctacttttg tctgcactat gaactaaaga accaattccc gtcaataatg ctactgatag
    14101 tactatactt aaaactatag atatagattg ttttttataa tgctttatat attgaattga
    14161 aaattttact agagacatca tagtacttca ccaccagtat tcaccacaac tccatcttca
    14221 atatggataa ttgagtcgca tacttgtgct atattttcat tgtgtgttac cataagtatc
    14281 gtttgatgga attttgcact actttctttt aataaacata ccacttccat tgtagtttta
    14341 ctatcgagat ttcctgttgg ttcatctgct aatataagcg caggtttatt tgccaaagct
    14401 ctagcaattg ctaccctctg ttgttgacca ccagatagtt cgcttggaaa ttttaaacgc
    14461 ttatcccaaa ttcccaatgt cttcatcagc atttcaatat attcatggtc tatatggttt
    14521 cctttatcta atgtaacagg aagagctata ttatcataag tgtttaatac gggcattaaa
    14581 ctgtagtttt gaaatataaa tcctatattt cttcttctaa acactgtcaa atcttttttt
    14641 cctattttag atatgttctt tcctttaatt attacatcac ctgaggttgg attatctaat
    14701 ccacctataa tatttaacaa tgtgcttttt ccagaaccag aagttccaac tatagcagta
    14761 aatgtatctc gttcaatttc tatacttact ccatttaagg cttttacttt agtctcacct
    14821 tttccatagt gttttactaa attattcgtc actaatatac tcatttaatt tctcctttca
    14881 atacagatat atgttctata aaatattgta agtgacgata attacactca tcctacaaaa
    14941 ttagtttaaa ctcttacaat tctgtaagaa gatactaaaa caacatccac ttcctagttt
    15001 agattctact ataatgtttc cattttcttt ttctaaaatt aatcttgtaa gatatagacc
    15061 aatccctgtg ccattttggt tttcaacatt tttacttctg taaaatcttt taaaaatatc
    15121 atttagttct ctactatcaa tacctattcc ctcatcttta atagtaatct tcgtatatag
    15181 ttccattttt attacagaaa tagtaatcgt gctatctgat ggtgagtatt ttattgcatt
    15241 ttctaaaaca tttacaattg cttctatggt ccacttttta ttatgaacta attttatgtc
    15301 tgaaaatggt tcgagattta tccttatatt tttagcttct gctttattaa agatagtact
    15361 gatactttga ataagagtat ctttaattag tagttcttct acttcaaatt ctataactcc
    15421 atcctctaat ttagtcattt taaataagga ttgtaacagc cagtcaatct taaaagtttc
    15481 atttttcatc ttttgtaaaa actccttctt ctgtaatgtg gaaatattta tattctccag
    15541 aagttcacaa taaatggtta tatttgaaag tggagttttt aattgatgtg acatatttga
    15601 tattaaacct ttaatcgctt ctttttctga ctttgactgt tctacctcta tttctatcat
    15661 atccttaatt ttttttactt ggtgagaaat ttttgacgct cttgtatcct ttgtatctgg
    15721 ataagtaact tctttacctt ccaatatttg atttaacatc aaatttaatt catcatatct
    15781 ttttcgattt attttagtag atactataaa tataacaact gtacatatta taaaggctaa
    15841 tataaacata ttattctacc tcattccata aataacccat tccataagct gtttttatat
    15901 attttggatt agatgggtca tcctctactt ttactctaag tcttcttata tttacagata
    15961 caatattatc atcaacataa ttttcttcgc tgtcccaaat atggtgtaaa atttgttcct
    16021 ttaataatat ttgaccttta ttttcaatta gataacttag aagcttatat tctacagagc
    16081 taagttccaa acattcttta tttttatata cttttagtaa cttttggtcc aattttattc
    16141 cattggaata tattacttta ttattcgatt tttttcttat tgctactttg actcttgcct
    16201 ttaatacaga taaagaaaat ggttttgtaa tgtaatcatc aagccctatt tctaatcctt
    16261 ttacttcatc aatttctgag tctcttgcag taagcattat aattggtata tctgactttt
    16321 cccttattat ttgacataca tcaaatccat ctccatctgg aagattgcag tctaagatta
    16381 taaaatctat atttttacta ttaaatatat ttaatccttc ttgttttgta taagccttaa
    16441 atacattaaa tccatcattc tcaaatgcat aaaaaagacc ttcatttaag tttatatcat
    16501 cttcaataat taataagttc aattttactc tcctttagat tttttagata aataattatt
    16561 ttcttgcatt atcatactaa aaatatttta attttattta tttcactaaa aagattgtat
    16621 ttaaatttgt tttcaaaata aaaacattat aatcaagcat atagttatat atttcccaat
    16681 ttaattacgc ttaaattgaa aatataactt tattcttata taattaaatt atacaacaat
    16741 aaagttatat tttttaaata tatttttact agggggaata gaattgaaaa gggttgatga
    16801 gagaaatact atgtttgcaa gagcaaatta caaaaaagat agtactgctt ataatgacta
    16861 ttataagaaa aatcctgaca aaaaagaaat tgatgatagt ataagaaata gaccaaattt
    16921 atgtagtgaa ggaacaatga cttacaatga gttaaactct ccaatggcaa gtagtgcatt
    16981 cgatttttta tctgatatca aatctttatg cgaaggaaaa gtttctgata ctaaagtaga
    17041 tgtagatgca aagtcaatga caaaaaaaat taagggtctt gcaaagcagt atggagcttc
    17101 tgtagttggt ataactaaat taaaagacta tcatatttat acaaataggg gtagacatga
    17161 agaaaattac ggtgaagaaa ttaatctaac tcataagtat gcaattgtat ttggatgtga
    17221 gatggataaa gaaatgataa atagagctcc aatgatatgt gaagttatag aaacatcaaa
    17281 gtgctatgtg gatgcatcta tcgtcggcat gattttaagt tactatataa gaaatttagg
    17341 atatgatgct agaaatcata tggatgctaa ttatttagta atgcctgttt ttatagctcg
    17401 tgatgctggt ttaggagata taggaagaaa tgctatactt acaaataaag attatggctc
    17461 tagacttaga ttaggtgttg ttactacaga tattcctctt ttagaagatg aatatgttga
    17521 ttttggacta gaagattttt gtaaggtctg taaaaaatgc tcttttaact gcccttctca
    17581 ttcgctttct aatgatatta aaattggtga tgatggaaaa tataattggg taattgaaca
    17641 tgagacatgc tatattaaat ggagatattt aggtacagat tgtggtatgt gtatatctgc
    17701 ttgcccattt agccaaaatc tcgaaactat aaagaatact acttcattta agggtaataa
    17761 tgagcttatt caaaaagctt tagatgagta tacttcaaaa tttggtaaaa gaatatttat
    17821 acctggaaat ccatcttggt tgaaataatt ttatttaata aagaatctgt actagaattt
    17881 atcataggac aggttcttta ttaaatatat aaatttttaa atactttttc tcgtttataa
    17941 caattcagca atgtttttag tctataattg acattataaa atattaaact aaaaggagta
    18001 actgtatgga aaaaatatta gtagtagaag atgattctat attaaataaa actctatcat
    18061 ataacctaat tgaagatgga tatataatta cttcaaaatt tacagctaaa tctgcactaa
    18121 agtctatttt tgaatgtgaa tttgatttaa taattttgga tattaattta ccagataaaa
    18181 gtgggtttga attatgtaat gaaataaagg aaaattacaa tataccaata atttttttaa
    18241 ctgctaatga tatggaatgt gatatgatta aaggctatga acttggagct ttggattata
    18301 taacaaaacc atttaatatc aatatattta aacaaaaaat taaagctttt ttaaatcatc
    18361 ttacaataaa aacaaaacaa gattactata gggatggata tcttgaaatt aacttttctg
    18421 agttatctgc caatataaat ggaaatcaaa ttatatttac accattagaa tatcgtactt
    18481 taaaactgct tactgaaaat cctaaaagta ttttaacaag gaaagtactt ttagaaaaac
    18541 tctgggatat agatgctaac tttgtagatg aacatacttt aacatctgtt ataagtagga
    18601 ttagaagcaa aattgaaaaa gacaatctta agtatatcaa aacggtatat ggtatgggat
    18661 atatgtggtt aggtgaaaga aatgaatttt aaaaaatttt cttttagttc tatttattta
    18721 ataattgctg ttggtataat ttttacagct attttattta gtatctcaat ttatctaata
    18781 actgatgatt taaaactaat tgctctatta ttaatatata caatagtact ttgtggattt
    18841 atagcattaa taatattttt acttcataaa aaaatagtta tattttgttc tagaatatgt
    18901 cacactttag atagtatgat gaatagtgaa gataatattg aaattgaatt agaagaagaa
    18961 actattttat cacgtatcaa tcacagatta attagaatgt atgaagttat tcaagaaaat
    19021 cgcaatagta ttgctataga gaaagctgat ttacaagaac tagtttctga tatatctcat
    19081 caaattaaaa ctcctattgc aaatttaaaa atgattaata ctactctact aaatcaaaca
    19141 tttaaaacag aaattcaaaa ggatttttta atggatatgg aaaaccaatt agataaatta
    19201 gattttttaa tgcaatctat ggtaaaaacc tccagactag agacaggtat aatcacacta
    19261 tctaaaaaga aaaattcaat ttatgaaaca attgctacag cacttagtgg aattttgttt
    19321 gatgctgaaa aaaagaatat tgaagttaca gttgattgtg acccaaattt atatataatt
    19381 catgacaaga aatggacttc tgaagctatt tttaatatat tagataatgc tgtaaaatac
    19441 acaagttcta atggtaagat acgtgtagct acagaatgtt gggttatgta tacaaaaata
    19501 gatattattg ataatggaaa aggaatatca gaaagtcgcc aagcagaaat ctttaaaaga
    19561 ttttatcgtg aagaagatgt ccatgacata gaaggtattg gaataggtct ctatctttca
    19621 agaaaaatta tatctcttca aggtggctat attaaagtta catcagatat tggaaaaggg
    19681 tcgacttttt ctattttctt acctaataaa taaattttta aatatttcat ttgtaatgat
    19741 tctgcaacat tttggtttta tactgtattt acaagatgtt aaggaggtat tattatgagt
    19801 attttacaag taattaattt aaagaaatat tatggacaaa gttcaaacat tacaaaagct
    19861 cttgatggtg taactctatc tattgaaaaa ggcgaattta tttctattgt aggtacatct
    19921 ggtagtggca aatccacatt attaaatatg atgggaggat tagataatcc tacatctgga
    19981 aaggttatta taaagggtaa ggaattatct actatgtctg atgaacagct tacaatcttt
    20041 agacgcagaa atataggttt tatatttcaa aattataatc ttgttccttt acttaatgta
    20101 tatgaaaata ttatcttacc aataggattg gatggagaga ccatagataa ggattttata
    20161 aatgaaatta taaatatgct ttctcttgaa gaaaaattat atgatatacc aagtaatctt
    20221 tctggtggtc aacagcaacg tgttgctata gctagagcac ttgtaacaaa accagctatt
    20281 atactagctg atgaaccaac aggtaacctt gatagtagaa ctagttcaga tgtactgagt
    20341 ttacttaaag tgacaagtaa aaaattcaac caaacaattg taatgattac acacaataat
    20401 gaaattgcac aactaactga tagaattatt cgtattgaag atggaaaaat tgttaactag
    20461 gaggtgtagt atatgtttag aaataataat cgcacattta taagaaaaat agctttaaat
    20521 gatttaaaaa ttaataaact taaaacatat ctttcaggta tcattataat gatttctaca
    20581 tgtttattat taacagtgac cttagtttca tataatgcct ctgtagattt agttaatgca
    20641 tctccatatc atgctatata taaatctgta gatgaaaaag caaaaaatat actatataaa
    20701 gataaaaact ttagtggcgt aggaacttac aaattaattg gaagtaataa aaagacagat
    20761 tgtattatgt cgattgttta tgcagatgat acagcaatta aacttatgaa ttttcaacca
    20821 ttgaaaggca aactacctac aaataaaaat gaaattgcta tttcagaaaa atatttacag
    20881 gaatttggtt tagataaaga tattggagat agcattaaat taaattatta tgatgatatt
    20941 acgaataaag aagttcaatg tgattttatt attgttggat ttctagaaaa ttattatcaa
    21001 gataatgcca agcaatacta tacagtcgtt tcaaatgatt attttaagaa tatagcttct
    21061 acacctttac aaaataaaag taatactttt gacgagtcaa gacctgatac tgtagatgta
    21121 ttagttaaac tatcagaaga aaatacagag aaagattcag cttcaattaa gacacagtta
    21181 aataaaattg gtttatcttt aggtattaag aaatataata tatacttaaa tgataactat
    21241 attgaatcaa atttaattga tggtgagcag attattacag ttttagtagt tggaatagtt
    21301 gttctttttt ctagcgtttt tgttatatat agtatttttt atatttcagt tgttaattca
    21361 gttcagatgt atgctaagtt gaaatcgtta ggtatgactt cttttcagtt aaaaaaaatt
    21421 atttctctac aaggaaatat tttgtcaatt atttttattc cattaggggt tatagcttct
    21481 tgtataattg cttacataat tcaaccatta gcatggcaaa tgatagctga tttatttata
    21541 attttaattt tatcactggt aatgttttta actgttagaa tttctttatt taaaccagct
    21601 agaattattt ctaaaatatc tgctattgaa gccatgcaat atacagaaac taaatttaga
    21661 aaaaaagcaa aaaattttag ttatataaat ataaaaaatc tagcattaaa gaatatcgag
    21721 tcaaatagaa agaaaaattt gattgcatta atatcgctaa gcattagtgg agttttattt
    21781 atttcaatcg ctaatcttgc taattcaata gatttcaaaa aacaactatc tcaacaattt
    21841 gtctataacg aagattatat aattagcatt aagagtgata acttatatga gcatattaca
    21901 gaaatccaac aaaataatcc actaacagat tctcttaaga atgaaattaa atctattcat
    21961 ggagtaaaaa aattaattga atccaaatca attaaaagta atattataga accttacatc
    22021 aaagatagag acgaaaaaca atttattact ctaattaaag gtattacgcc tgaactttca
    22081 aaatcacttg aagaatatat tgtcagtgga aaaatagact atacacattt agattcagat
    22141 agtttaatta taaataaata tagaacaaaa tattatggat taaatttaaa agttggagat
    22201 acagttacat ttaatatctt tagtggaaat gatattatta aaaagaaaat gaaaattata
    22261 ggaatcattg acaattccaa tacaggaggt atgttttttg cttcagacaa aactttagaa
    22321 tctctaacac catataatac aaatttagat ttttctattt taatagataa aaagcattct
    22381 aaagaagttg aagaaaagtt gttaagtata attcagaaaa attcaaattt aatgctttat
    22441 tcatatgaag atgattatag aatgattact agagcatttc aatatataat tatagcttca
    22501 tacatttttg ttggtttaat ttcttgcttt ggtatactaa atatgaccaa taccctaata
    22561 aacagcgttt taattagaaa aaaagagttt gcattacttc aagccgttgg aatgacaaga
    22621 aagcaactgc gaaatatgct ttatagagaa ggtctaaaca taagtatcaa agctatatgt
    22681 acttcaagta tactaggtta ttttggttca aatctacttt gcacttttat aaaagatgta
    22741 attagattag actttattaa ttttaaattt tctatattta ctattttaat tttttcattc
    22801 gtattaattg gtatacaagt tttagtgact gaattacttg taagaaatat agaaaaaaaa
    22861 tcacttacag aacgcttacg ttctcagtaa gaatgtatat aaaaaatagc cattttttat
    22921 acaagtttta acataatttt ttaaaagcta aaaggataga aaattttgac tttacataat
    22981 tttctatccc tatacttgtt tttattttat acagatacac ttacttatat ctccgagcaa
    23041 catcaccaga gacataaaca agcatattcc ttattttcta aatatgtcaa ttgcatgact
    23101 tgtaacccct actaaatctt cacgttcaca agtagcaagg tgatatttta aatataatgc
    23161 aaatgcatca tcatccatag agtctactgc ttcacgcata tacaaagcca aaccatctga
    23221 tgcgacataa tgcagtctag ttacattaaa tgcagacatc aaatcatcaa tgttttcttt
    23281 acgtacaagc tcaaacaaat cctttggttc agacttagca gcaaatgtct gtggatcaat
    23341 caaacctttt tcaatatatt cagatacatt aatatttcct ctatggaacc cctcgtcaat
    23401 aagacagcca tcagatataa catatgcagc aaaaaccaca ccaccaggtt ttgttacacg
    23461 aattgcctca tgtaaagctt gttgtttatc ttctttgtta taaagatgat atagtggtcc
    23521 taacagtaac gtaatatcat atttattatc tgggaaaaca gataaatcca tagcattacc
    23581 ctgagtaatg cttatatttt catttgattg agtattttta tggaaaacct ctatattatg
    23641 ttcgactaat tccactgcgt caacatcata tccctgacgt gccagtgtgt gagaataacg
    23701 accagttgca gcgccaattt caagcacaca atcacctgat tttatgtact tatcaatgta
    23761 atgcatagta gtgagaaatt ccaccattcc atgttttaat gcaagtcgac tatcttcatc
    23821 ataagtatta taaaagtcaa ttaagtattg ttttgtttcc ataatttcac ctcttctata
    23881 ttagttaata aacatcaaat agacaaattc caatctgatt tttcttgtct tataaaagct
    23941 gttaatatat ttttcaacat tttttcagca ctatatctat ctaatgttaa tttttttgtt
    24001 gctgttaaga cagctatgcc atgagtataa agaaataaat ctaaaaatat tacttttgca
    24061 cattccaaat ctattccaat gctttccgaa aaaagtcttg cctttttttc attgtcaatt
    24121 tctttataaa aatcattcgc ctctttcatc atcaaatcta aatcatttat aaataacaac
    24181 ttaaataaat acgtttcttc ttgagcaaat tcaatgtatg aaagcggaag cagtaaataa
    24241 gggcttacac ttttagaatt acgataatta acaacatact gattataata ttcatatgca
    24301 aaagcaagaa attcattttt taattcattc atgttttcat aacaagtaaa aattggtcga
    24361 gtggaacact gtaatttact tgcaatactc ctcgcattta cagcttcaaa tcccacttct
    24421 cttgtaatat ccaagactgt attcaaaatc atttcttttg ttatctttac tttaggtggc
    24481 atatatgttc acctctccat taaattttta taaaacacat gtgttatata actatagtaa
    24541 catatcaaga aattatgtca agaaaaagct ttgcattttt ttgcaattcc taaaacttat
    24601 aaataaaaca tgagttgcaa ttaatctatt aatagataac agcaactcag attttccttt
    24661 aaatactctt ctttttgtct aatatttcat atacacaatc ttctattgaa ttaatatctt
    24721 tttcttttct ttttttagaa tcaattaaaa tgaaatacag tataaaacct acagcaagtc
    24781 cccatgttcc gcctttagca gcaattacac ctcccataag tccaatcaat cccttctcaa
    24841 cacctgtatt acatagctcc attgatagct ttgtacataa atatccttga actaaaagtg
    24901 ttactgacaa tgctaatggt ataagaggtg tagcaatctg cgcaattgga taaaaacaga
    24961 taaaaattgc tgaaacccat actaatgtac ccatgccact aaaaacagac tgcatggctt
    25021 cctttccaac cttgtacctt tgagcaacac ttcctgtaag tgtcgcagct aatggaccgc
    25081 acattggtat atatggagaa aagattgcca taaaaatatt tcttatccca cttataacat
    25141 tagacctatt tgaattgaaa tctatatatt catcttttct attttcactt tcagaaagta
    25201 ctaagttttc acctgttata aaatcaccaa aggcaattat gtatatcata aaagccattg
    25261 gtatcccttg tataaataac attacacttg gaaaacctat tccaaaagga gatactgtat
    25321 ttatcagatt tttaaaatct actatgtaaa ctaaattgtc aaaactaaat ctaggaattt
    25381 ctatttcttt actaaatata cctactatta aactcactaa aatagctgat actacaccat
    25441 attctcctaa atgaaataaa aatttattct taacttttag agtatcaaat ctttcagaaa
    25501 acattacaaa acaagcaacc aaaattccta ctgttataga tataggataa gaatcaaatc
    25561 tccctttagg attaaactct gatattatag tcgatatacc gacacccatt agtattccac
    25621 ctttaataga atttggtatc ttactaacta actttccagc tacaccagtt atacccatta
    25681 aaataaatat tattcctaaa tctatttgta aagctgttaa tgcttgtatt ctttcaatgc
    25741 ccatgctgaa ctttaacaaa tatgcagttg taagagttat agaagctgta atccagccag
    25801 gtacaactgg gtctcccata aaaacatgca aacagtataa tattgagtta atgatagcca
    25861 tactccatgc cacttcatag ggtaatccca aaatttgtgt actaaggcca attgtgccta
    25921 aacttgtcat acttgatatt ataccttgaa tagcttctgg tttctcaaat ctgtaatgta
    25981 taaatggaat tcttatctta aacattccaa attttatatg aggttgttcc cttctagcga
    26041 gtctttccat aacttcactc cacctttcaa taactcttat aagtttacaa ctttataatt
    26101 gtttataaat tatcaatgtc attaaaatta gtatataaca tgcaaattat ataaacaata
    26161 aattaaatct ataaaattga tagattttgt gcatatatag aaagaccgct tcatttttag
    26221 ttacttaaat cacttaatag tagttattta aatcacttga taaaggtttt ttataaaatt
    26281 caatagttct attccatgat tcaaactgtc cttttgaatt tccttctaca gttccacctg
    26341 aagaaaaaat ccctccactc atactcatac ataaagtctc ctttagaggc ataatatatg
    26401 gaattggtaa cggatgaccc atattattgt atgttaataa ttctacgtta tatggataat
    26461 tattttcttc aaagatttct aatgccttta aacaagcttc ataactattc catatgttat
    26521 cttcttttcc agcaatcata agtatatctg ctttggaatt ttctattttt attctagcct
    26581 cttttctatt ctctgctctt ttaagacttt tcttataagt actattaaat ccaaatggaa
    26641 taccttttga tatatttttt ttctgttctt caaagaatat atcattgtct actttgatat
    26701 atggaaatgg ctctccttta tatgaccatg atgaagtatc atttccactc atcaatccat
    26761 taagagcttg gaaacaataa atgtgaggtt gtgatactac aaccttttta atttgttcat
    26821 atcttgaagc aagtaaaaag gccaattctc caccctttga tgtcccatgt acaaatattt
    26881 ctttagtact ggtaatttca ttttcactaa tccactcaaa tactttttca aaatattcta
    26941 atggaacctc ttctaattta tctggtaacc cttctaaagc aaagtatgca actgttagta
    27001 cattgaatcc tctagatgca agtggagctg ctaaaaggtc tagagcgtct aaatttccat
    27061 cagaaccacc tagcattaaa attgttttat tattagaatt ttgtttataa aataattttc
    27121 ctataaactc atcagatata tattttgtgg taatatcttc tgatttaaat attctcttta
    27181 attcaacttc ttttctttca atagaagttt caaatatcat attcataatt attggtttat
    27241 caatagatat attttctgca aaatttttac cttcagtttt agatttttcc agcgaataaa
    27301 ttaaccccat acaattatca gccttataag taccttctat aggttcaact aaatctaaat
    27361 ctacttctcc tttttcattt gaacaaaaca ctccataaga tgaaaattct tctcccttgc
    27421 accatggaaa ttccattttc acacttattt taagtttagt atttggaatt aagtccgaaa
    27481 ccataatctt aagtctttca tccacaaatg attctacatt gtatacattt actttcataa
    27541 tctatttctc cttataataa ttatttatgt tttatataaa acagctttat ctaatacaac
    27601 ataaataaat ttttaaatta agacaccata ttgactattt cattttatca acatattcta
    27661 tttttgtcaa catattgtct tttttagcaa tttaaaatta tgtagtctat atgaaatcca
    27721 gttgattttt ggctaattaa agataggaat gctattaggt ttagaatata tatattcaaa
    27781 ataaaaaacc actcatttta agaatggttt ttaatataaa ctatttgtta ttaataagga
    27841 tattttttta tttaaattta tataatcgaa catctaatat ttaatgttta ataaaattat
    27901 gcatttgcaa aagtatatat ggcattaact cttgctacat ataaattctc atcactacat
    27961 ccaatatatc tagcatattt ttcaatcttt tcttttgcat tttcaacatc attttcatcg
    28021 ctctccatta actcatttat ataaatatcc attaatgacg ctttatattc tcttatatca
    28081 tagcctaaaa attcagtcaa tctctcaatt tccttactaa aatctccatc ttcttttacc
    28141 aaaaattcgt atacatcttc cctatatgga tttaaagtta ttatttgttt tactactttg
    28201 cattcgtcag ccttcccaat aaaaccatct ttataattat taaataaggc attagatttt
    28261 tttatagatt cttcatcaat ataagaatgt accgcagtta tacgattttc tataagggca
    28321 tctacaacag cataatgaat tttaaaacaa tcattgtata aataattgct cattttcttt
    28381 attgtcgatt ctatttctaa gaagctcgcg tggccatttt ttttattttt atttggtata
    28441 ataaaagttt tattcaagtt attaaaaagt ctcttaaaat ctaaatattt ttcacaataa
    28501 acacttttaa aggtattgat atctatagtt ataattccaa aacttactat agtctcaatt
    28561 cctttcttaa aagactcttc tatatatttt tctcctaaac ttaaacctct atcagatatt
    28621 tgctttagat tttgaaaact atttaaacaa tctctctcaa agtttttaga tatttccaaa
    28681 gcatataatt taaagatttt ccttatctta ttataattat tttttgtatc atcaaatctg
    28741 ataatagctc catttacatc aaaagtaatc attgagtttt aacctcccca ttagtttcat
    28801 caataaaata aatttaatat aaactttata atcataatat taaacttatt tatactataa
    28861 ttattaattt gatgataaaa gctattttcc ttctaaataa aaaatttgtc taaagtttct
    28921 aaaaaattta gacaaatctt aggttattac tctttaaatt atttttaaat tatttttaaa
    28981 ttattccaaa tacgccaaat aatattggta taaatatagc tggtaacaaa ttggctactt
    29041 ttatattcgt taatttaagt acatttagac caagtcctat aattaataaa cttccagctg
    29101 ctgtcatttc tgtgactaca ggatcactta aaaatccatt taaaaatgat gctcccacac
    29161 atataatacc ttcatacaaa aatacagata ttgcagaaaa cattacacct atacctaaag
    29221 aagcagtaaa tataattgaa gctattccat ctataacaga ttttacaaat aaagtatcat
    29281 gacttcctgt aagaccactt tctaatgaac caactactgc catggctcct atacagaata
    29341 ataaactaga tgatataaaa ccctctgcta tagaatcttt tttatttcct ttagaaaatt
    29401 tactttgtaa aaaagcaccc aacttattta cccatttgtc aatatctatt aattctccta
    29461 tcagagcacc tacagctacg gatattattg tcactaatgt gttttttcct tctagtgctc
    29521 ctgatatacc aatgtacaat acacataagg caattccatt cattatagta tcgcttactc
    29581 tttgaggtaa tcctcctttt attataagac ctactataca tccacctgca attgctagac
    29641 tatttgctat tactccaaac attttttctc ccctcgatta gctaattgaa atttatccta
    29701 ttattaaaac ctaaacatct atactttgta ttggatattt taaatattcc aaaaattctt
    29761 caaaaccttt tctagatata tttacagttt tagtatttat gttaggatga aaatttaagt
    29821 aatcttcttt aattacatct ttatctatgt aaagctcaac ttttctttct gtatcattta
    29881 ttaatgaaaa aggatttaca cttccaggaa gaagtttaag tacattgtat agtctttcag
    29941 gtgaagcaaa cgataatcta gaacttccta tcttatcttt aagtgagttt aaatcaactt
    30001 gtttttctcc tttaactaca ataagataat acttatttcc tttggcattt cttaaaaata
    30061 aatttttaca ttgacacccc tttgttattc catctaaaat gactaattgc tctgctgtat
    30121 atacagcttc atgctctact acatcatatt caacatttaa tttatccaaa atatcatata
    30181 ttttagtttc attcatgcta gattcatttt catcttgttc ttttttatta tttaaatcac
    30241 tcatactttt ctccttaaac acttcatcat aatatcattg tgaggtatta ttctactata
    30301 gctattgctc ttactggagc tccatcagca tcattatact taaatggtgc tgctataaat
    30361 agaaattttt ctggaacatt ttctaaattt gtaagatttt ctatgattat ttttcctttt
    30421 tcaaataaaa tatgatgcac ttcaaatact gatgtgtcat atctatccac tgatagcata
    30481 tctattccta tgccttttat attagtatta gctatatagt ttgcagcttc ttttgttaat
    30541 gtaggataac ctacatagta ctgcttttta tcccaaaatt ttgaccaacc tgatttaaat
    30601 attataaaat cactattttt tatttcatct tcatattttt ttagatattc taattctata
    30661 ttctcatttc ccttttcaag ttccaataca taagctttcc caacaaaatt ttctatatcc
    30721 aacttatcta gggtctcacc tttagtatac atatgtcttg gtgaatccat atgagttcca
    30781 ttgtgagaaa atacactaat taatgtttct tgataaccat tttcttcaat aatagctact
    30841 ttcttgatat ctggtctatt aggctctgca tatacaggca tgtcattatg tgtcacatga
    30901 gttaaatcaa aaaccttcat ctttatatac cccctatgtt tttattttct attatatacc
    30961 taaattcatt ttatttctat cagattttat tccattatat accatactac tatatatttt
    31021 ttataaacct caagggattt accgaaaaat cttggaaaga attatcagta aatataacat
    31081 cttcacccaa aaaacgttta taccttcctt cttctttctt taaagtaatt ctctgttttt
    31141 gtgaatgatt attaaaaacc attatcttaa gaggaggttt ttttctatta gtctgcactc
    31201 tttttaacat gtattttatg tgcatatcag cttcaggaaa tgaatatcca caaaatatta
    31261 aaaggtcagt atctcttaaa gttttttctg tttcattcca aacatcactt aaaaatatgt
    31321 ttgacatatt cttaaaatat gttggtggca ctataatagg ttctgttaac tcaccacact
    31381 ctaaacattt tgtctctgaa ctattttcaa taagcttcat gacaccacct tcatgtggtg
    31441 taagagttac actattacaa atggggcaat aaagccaatt caatgaccca tgtatcttat
    31501 ataatttgat taatggttct tttggttttc tccaatttaa ctcctcttta aaatttgtaa
    31561 actccacacc ataatcaagc ataattggca aatcattctt atctcttaat ccagtaactg
    31621 tattgtctat atgtatatca taatttgcac taataaaagt agtatcaaga agcaaatcat
    31681 tttccaaaag attttcaact aaaagtttat gatatttatt atctgttcta ggtgtattat
    31741 gaattgcttc agccattagc agtattaaat attgtcttaa aaaacgtata ctatcacttc
    31801 tgttatttag attttctagt ccaaaatttc taaatgcttc ttttctttgt tctgctaagt
    31861 ctaaaagacc taaaacttct tcaaaggtag gaaaatttgc attatctata ttatctttta
    31921 ctatatcaat attgaacatc tgtttaaaga aaatatacaa ttctctattc attttggtat
    31981 tataatttgt gggatttagt tttttaaaat attcgctaaa aagttcattt tgaataggta
    32041 gattctctgc tgctgaagct cctgctccta aaaatattgc tattttcata tagttcctcc
    32101 taatataaat attactccta tttttatatc cattaaatat ccatatcatt tattttatga
    32161 tacaacaata aagctgtctt ttactagaaa aaatcctatt acaagacagc cttatattat
    32221 attaaatctt taataattcg taaatagaaa gagcatatat ttgagtagct tttataatat
    32281 cctctactat taagtattca ttagcttggt gttctgtttc tggttttcct ggaaaaatag
    32341 ctccaaatgc aacacaatta tctaaagctc ttgcgtatgt tgctcctcca gatgaaagtg
    32401 gtgttccatc aagaccagtt tcttcttcat atacttttct aagtgtctta acaagaagag
    32461 tatcttctgg aacatagatt gaatccaaga aatcatattc ttcataattt aaattgtatt
    32521 tatctgtcat tttctttaac tcttttacaa aatcatcctt tttgtatgtt actggaatcc
    32581 ttacatctat tcctgcaaat tctttttcgt tatcaatagt tactctacct atatttactg
    32641 ttagtttacc tgatacatca tccttgcagt taggaattat attgtttcca ttagcatctt
    32701 caccaataac ttctgctaaa aacttaatta tattagaatc tatacctata ttattaagag
    32761 ctatacaaag tctagccact gcatttatac cagtatcact tgctgctgaa tggacactct
    32821 ttccaattat acagatttta ttaccttcaa cagtatactc aaaatttaat ttattaagtt
    32881 ctttttttaa tttatcagag tattttccaa tatagattgc tttacctgga actgcattta
    32941 aagcctttcc aactgacaac tctatatcac ttttgctatc acaagtcaaa tgaacttgta
    33001 aaagaccttt ctctgcattt gttattggga atctagaatc tggagtaaat ccatagtttg
    33061 gtatttcttc attattttct ttatatttat ttatacatct ccaaagattt tcttcatcag
    33121 ccccaaaaat gaatcttatc ctcttattaa agtctacatt taaatccata agagctttca
    33181 ctgcataaat tgctgatatt gtaggtcctt tatcatcttg tgttcctctt ccatacaatt
    33241 taccatcttc taaaacagct tcaaatggag ggtaattcca gctttctaaa tcaccttctg
    33301 gaaccacatc aacgtgacca agtataccta tcattttttc accttgtcct atttctgcat
    33361 atccataata tccatctttg tatactgtct taaatcctaa agatttacat aaatctaata
    33421 tacctcttaa agctttatct acattttcac caaatgggca attttcagtt gcttcagata
    33481 taacactagg tatttttaca ctttcttgta ttgaagaaat catttcatct tgtagagaat
    33541 ttactttttc tttaatttgc tctttcatat tatcaccctt caattttcat cataatatac
    33601 taataaatat tttgaccttt taaaaaaaaa atatgcttat tttttcagtt tttttataaa
    33661 ttcaatcgct tttaaagtcc ccatttttga atggtcaaat gtaagtccac cttggaaata
    33721 aacattataa ggaggtctta ttggagcatc tgcacttaat tctatagaag aaccttgtat
    33781 aaatgctcct gctgccatta tgacttcact ttcgtatcca ggcatgtccc aaggaacagg
    33841 ttttacataa gaatcgactg gtgcagcttc ttgtatacct tcacaaaaac ttattacttc
    33901 atcagcatta ttaagtctaa tacattgaat aatatcactt cttaagtcat catattttgg
    33961 taatacatca tatcctaatt tttcaaaagc tcttgagcaa aagatagctc ccattacagc
    34021 ctgagatact atataaggcg ccaagaaaaa accctgaagt acatttctag ttgttccaaa
    34081 agtaagacca cattcttttc ctattccagg agaagtcatt ctataagata taagctcaat
    34141 caaatctttt ctaccagcta tatatccacc tgtcaatgca agcccaccac caggattttt
    34201 tattaaagag ccagccataa catctgctcc aacatcagta ggctctttgg tatctaaaaa
    34261 ctcaccataa caattatcaa ccattactat agcttctggt ttaacagact ttattacttc
    34321 tatagcttct tttatatcac taattgaaag tgattttctc caagaatatc cttttgacct
    34381 ttgtatcata acgagtttag ttctatcatt tattttgttt ttaattcctt ctaaatctaa
    34441 gtttccatcc tctaaaaaat ctacatcatc atatgtaaca ccatactctt tcaatgaacc
    34501 tttttcttct cttatgccta taactccctc taaagtatca tatggtctac cagtaacaga
    34561 taaaatttca tctcctggtc taactatacc ttgtatacat aaagtaagtg catgtgtacc
    34621 atttacaatt attggtctta caagagcatc ttctgtatta aatactttag aataaatttc
    34681 ctctattttt tcacgaccaa tatcattata tccatatcct gtagtccagt taaagtgcat
    34741 atcacttagt ttagattctt gcatagcttt taaaacttta tattgattat attctcttat
    34801 ttcttttatt tcttcaaact tatcttttat ttcttccatt atttcttgcg atagtttaaa
    34861 agtatcatca tctattccat agtagtcttt taataattct tttgtctcat taagcatttt
    34921 atcacctgtc tctttattat tttatttaaa catgtaaagg agactatttt actaaaacaa
    34981 ataaattata gtttataagt tagtataagt ctcctctcaa tcatcttaaa ttaaattaat
    35041 tatctatctg ttgttattat tattgttgtt ttggttttga tttgttagat taatatactt
    35101 tccaggttgt atagttgata cagcatgttt ataaatcatg ttttgttgtc tattatcccc
    35161 ttctattaat attatataac tatcaaaccc ctttacaagc cctttaactt gcactccatt
    35221 aactaaatat atagtaacag gtatcctttc tttccttgca ttatttaaaa acaaatcttg
    35281 taaatttaaa actgtatttt tcattagatt tgtaccctcc caagattaaa ttgaacttct
    35341 aaaatattcc taccttatat tctatataag ttatgacaaa ttttcaatat taagttgtat
    35401 acttaaaaat ttatatatta ttttatagaa tctttaatat ataagattat ttcatttttt
    35461 aattcgtcta tattttcata ttggtctaaa tcaaaccatt ttgctgtttt atatctttta
    35521 aaccatgtta tctgacgttt tgcatacctt ctagaatctc ttttaattat ttcaatagct
    35581 tcttcgtatg tatattctcc atccagatac tttaatatct ctttataacc tataccttgc
    35641 attgatatca tatctttttt aaatcccata cttaaaagtt tttttacctc ttcaaccaat
    35701 ccatttttaa gcataatatc aactctcata tttattcttt gatacaaatg ttctctatct
    35761 ctatttagaa ctattattat tggttgatat tcttcattaa attttaaatc acttgaaaaa
    35821 tcattcattt tcttaccact tagacaaact tcaagagctc ttatgaccct tttagtattg
    35881 tttttatgga ttctattagc agattcttca tcaagttctt ttaacttatt atgcatgtaa
    35941 tctattccct tttcagcaag ttgtttttga agttcttctc ttaattcatt attggcatca
    36001 gattgtgcaa aatccatatt atatattaaa gagtttaaat aaagtcctgt gcctccagtt
    36061 actaaaacac atttttcctt tttatttatt tcatgtatat atttttttgc tctttgttga
    36121 aactctgaaa ctgaaaatgg atagtcaggc ttaacttcat ctataaggta atgcttcact
    36181 ccttgcatct cttcctctgt aacttttgca cttccaatat ccatatattc atatatttgc
    36241 atagaatcag ctgaaattat ctctgcattc atatcttttg ctaattttat tgacaaatct
    36301 gtttttccaa cagcagttgg acctgtaagt attataagtg gtattttttt cataacattc
    36361 ctttccatat attttaaata tccaaatatt ttacataatt cttttaaaca ttttttctat
    36421 ttctgtctta gatatttcga ccattatagg tcttccatga ggacatgtaa atggattctc
    36481 acacttttct agctgttcca ataaactttt tatttcaata tcgtatattt tatcatttgc
    36541 ttttatagca gacctacatg ccatagatgc aaatctctct ccttttaaat cataattact
    36601 tgtaatttct tctatattat caattatttg aagtataaat ttctcggttt caggtactcc
    36661 aaatatagta ggaacacatc ttaccattat atgattattg ccaaaaattt ctagctcaaa
    36721 accaaatttc ataaataact ccaagttatt ttcaatttga agcatatcta catttgatac
    36781 ttcaataacc actgggtcta aaagtatttg catatttata tcttgtctat agaacttctc
    36841 catatatctt tcatatagta ttctttcatg tgcagcatgt tggtccaaca aatacataga
    36901 atcgtctttg ctcaaaatta tataagtatt aaatacaact ccaattacag aatatccgta
    36961 taaagaaaac ttgcgttttg aactacacaa gtattcttct tcactgtcct taatactatc
    37021 tcctaagtaa taatttcctt catttttaat tccatcaact tgaaactctt cttgtatatt
    37081 atcatttgca ctatcttcac ttagataatt aatgtttttt acatcatctt ctacacttgc
    37141 atttaggact tcacttactg attgaaattc atttattggt ttttcacttg ataaagtaac
    37201 aacttcaatg acttcatcct tacctttagt aatattacta tttttaggag tagattctaa
    37261 taaatcatta tttcttaatt tataatctgt acttttttct ctactgttaa tagcgattct
    37321 aggttgtgtt tttttatcac tataagtagc atattttcca ataagatttg agtgtattaa
    37381 tttaaccttt aaaaaatctc taagttctat atacacttct tgttcttttt caaatttaat
    37441 ttcaagttta ttggggtgta tatttacatc tatacatgat gggtctactt ctatatttaa
    37501 gaagcacact gcatgtttac ctattggaat tatagactta taactttctg ttattgcatc
    37561 tatgattatt ttacttttta caaatctttt atttatatag atatgttgta agtttttatt
    37621 tgaacggtat atattgttat tacctatata tccattcatt ttaaagtgat tgcacttaaa
    37681 ctctacatct attatatttt ccgttatttc ttttccatat atacttctaa tagtgtttac
    37741 aagctttcca tcccctggag tatttagcat ctgtttattg ttatttgtat atttaaattg
    37801 aatgtttgga ttacctattg caagtttatt tatcaaatca cttatattta tagtttctgc
    37861 atgagtagat tttaaaaatt tctgtcttgc tggtgtatta aagaaaatat cttttataat
    37921 gatggtagtt ccattagtag aacctattgg ttctttcgat attatttttc caccttcaac
    37981 atatatttta gttcctatta tttcatcttt agttttagtt gtcatttcta attttgatac
    38041 agctgaaata gatgccaatg cttctcctct aaaaccaaga gagtataaat catacaaatc
    38101 atcaattttt ttaattttac tagtagcatg tctcaaaaat gatttttcaa cttcacttga
    38161 aggtattcct attccattat ctgtaatctt aattagactt tttcctccat caattatatc
    38221 tatggaaatt ttatttgcac ctgcatcaat ggagttttca ataagttctt ttactacaga
    38281 agaaggtctt tctacaactt cacctgctgc tatcttattt atagtcaaat catctaggat
    38341 gttaattata tttttcattt ttacacctct aagattttat ttcttttgct tttctttgaa
    38401 gattataaag agagtttata gcatctaatg gagtcatatt taaaatatcc agatttaaaa
    38461 tttcttctga tagtatatct ctatttacac tatcaaaaga gatttgagtt aaggctactt
    38521 cttttacaga atccacttga ttaattttct ctttatcatc attttgcttt attaaagcaa
    38581 cttcatcatt taatgcttta aatttagaat ttaatttatc ataatccttt tttatatgtt
    38641 tgtagtcttt tactataagt tcatgttcta tttttagaga ttcatattta ttcttaaaat
    38701 tactttgatt taccttaact aattcttcgt ccaaattact attaattata ttattgtttt
    38761 ctttatctcc atttattgca acactgttgt aaacatgatt tttttccaag tctttaagta
    38821 tatattttgc tctttctatg acttcgtctg gtaatttagc taatttagca acatatattc
    38881 cataactctt gtctgctccc tgaggaatta tttttcttaa aaatattatt ccttccccat
    38941 cttctttgac tgctattgaa taatttttaa cctctttaaa ctcttcttct aaatcagtaa
    39001 gttcatgata atgtgttgca aataatgtct tacatctaat atttttttga atatattcca
    39061 ctattgacca tgctaaactt ataccatcat aggtactagt tccacgacct atttcatcta
    39121 atataactaa acttctctct gttgcatttt ttaatataag ggatacttcg ttcatctcta
    39181 ccataaaagt agattgacct tgagataaat cgtcactagc tccaactctt gtaaatattc
    39241 tatctaatat tggaatatca gcagactctg ctggtacaaa tgagcctata tgtgccataa
    39301 gagctattat agcagtttgt ctcatatatg ttgatttacc tgacatatta ggaccagtta
    39361 ttatgttaat aatgttttct ccacgattta aataagtatc atttggaacg aaattttcct
    39421 ctcctactat attttcgaca acaggatgtc taccatttct tatatcaagt ttattatttt
    39481 catttattgc tggttttaca tagttattta tatgtgcaac tgttgcaagg gacacaaaaa
    39541 catctatatt agctatagtc ttagccactt tttgtattct atctatgttt ttatatattg
    39601 tatctcttat ctctacaaat atctcgtatt ctaaagattt aatcttttcc tctgcatgta
    39661 gtatcttttc ttctatttcc tttaattctg gagttatata tctttctgca ttactaagtg
    39721 tttgctttct tatatatgtc tcatctagct ttgcctgttt aaagttagct tttgttattt
    39781 ctatataata tccaaaaact ttattaaagc ctatttttaa tgatttaact cctgtttttt
    39841 ctctttccct gttttctatt tcctttacaa gaaaggctcc attcttagaa atctctctta
    39901 gttccttaag ttcatcacta aaatctgatt taattatgtt tccatctttt atggttatcg
    39961 taggttcttc tagaatagct tcatctatta aattgtatat atcatctaat ttatccattt
    40021 ctgaaacata ctcttttagg atttttgcat ttgataaatt tatagtatct tttaaattag
    40081 gtaacttttc tatcgaattt tttaagtgaa ttaactcttt tggtgttact ctctcaaatg
    40141 ctatctttcc acatattctt tctatatcgt atatattttt aagtatatca tttaggtctt
    40201 ctcttaatat aaagtcatct tttatttctt ctattacatc taatctattt tcaattttag
    40261 atttatttat caaaggttct tctacatatt ttcttaaaag tctacctccc attgctgtag
    40321 aagttttatc aagtacgtgt aataatgaac cttttttctt attccctctt atagtttgag
    40381 taagttctaa attagttcta gtaaacatat ctagaaccat atattctgat gaattgtata
    40441 tatttatatt attaatgtta gatgttattt gtttttgagt attgtatata taattcagaa
    40501 gaattgataa gcttgacttt attagacctt tatcatcaaa ctttagtttt tgtagataaa
    40561 catctggaaa atattctttt aatatattta tatccaaata gttatcacta aaactttcat
    40621 ttatgtatat attacttaca gtagctatat ctctaagatt ttctataaag tctaaatcat
    40681 ttataattat ttcagttgga tggatttttg caatttcttc aattacctta tcttcattta
    40741 aacatgttgc attagtttct cctgtactta tatcaacata agttagacct atattagtac
    40801 catccttata caaagataat aaataattat tctttttatt ttcaagtaaa tttccatcta
    40861 aaactgtacc aggagtgatg actcttataa cctctcttct aacaattcct tttgctgtag
    40921 atgggtcttc catttgttca cctattgcta ctttatatcc attttcaact aattttgata
    40981 tatatgaatt ggctgagtgg aaaggaacac cacacatagg agcacgttct tctaaaccac
    41041 aagcttttcc tgtcaatgct atttcaagag cttttgaagc tactaatgca tcttcaaaaa
    41101 acatttcata aaaatctcca agtctaaaaa acagtataca gtctttatat ctatttttta
    41161 cttcaagata ctgtttcatc ataggagtta atttattcat atctatttgc attatcaaac
    41221 ctccttcttt tctaattaaa tattatatca tagtagatga ttattattaa cacaatatat
    41281 aaaattttga catataacta aaaacaatcc aaaatgtata ctcacatttt ggattgctaa
    41341 gtttaatgct tattaaataa aaatattttt tttacaataa agatattatt tcttttttaa
    41401 gtttaatgat attttcaagg ttatcttcat ttaactttct actatctata acaaattctt
    41461 tctttactat tgatggtttt tttgaaataa cataaattct gtttgatagc attatagctt
    41521 cgtcaatatc atgagttata agaagtattg ttgaatttac ttttttctta acatctaaaa
    41581 gccatttttg catggatgtt ttagttaatg aatcaagtgc cccaaaaggc tcatctaaaa
    41641 gcattatgtc attagaattt ataaaagttc tcaagaagtt agctctctgt ctcattcctc
    41701 cagaaagttc tgaaggatat ttatcttcat atccgcttaa tccaaacacc tcaaaataag
    41761 gttttaccat tgacctagct ttactctttt tttctttttt taaaattagt ggtagtgaca
    41821 cattatctat tatagtttta tatggcaaga gtaaatcttt ttgatacata taactatact
    41881 taccatttat attcactttg ccactatcat aatctgtaag tttagttata atattaaaaa
    41941 tagtactttt tccacatcca cttggcccta atatagaaac aaactcacct tcataaacat
    42001 caatacttat atcttttaaa acttcaatat ctttataagt ttttgataca ttttctatag
    42061 atacttttac ttttttattg tggtaaaaat tcatttgtgt aagcatcact tgccttcata
    42121 tccttatcta ttaatttata ttcttttaag aatgatgtat agttgttcca tacactatct
    42181 ttcatttctc cccatcgtgg tgcatcatct ttgtacttgc ttgctaaata ttcttgactc
    42241 tttaaagcta gtttttcatc tacttcaggt gcatgtttta ctaaaatttt tgctgattct
    42301 tctggatttt ttattgcata ttcataacct tttgttgtag cttttaagaa ttttttagca
    42361 agctctggat tatctttaag cacagtttca ttagaaatta taagtggtgt ataataatcc
    42421 aatctctcat ctaaatcttt tactggtata aagtttaagt catatccaat taaatcagct
    42481 ttaactgcat cccaaccttc aaatatccat gcaaaatcta cagttttcat agctgcaaag
    42541 aaatcatctt gaccagtatt aactatcttt aacttattaa aatcagctcc atttttcttc
    42601 ataactgctt taaaaacagc ttcttctgat ggagagcccc aaccaccata agtttttcct
    42661 tcaaaatctt tagctgttgt tatattcttt tcttttggtg atgcaaaccc agaagtatta
    42721 tgttgtataa cagttgctac agcttttatt ggaagtggat cttcactagt ctttgcataa
    42781 gtaacttgtt cttgatagct tataccaaag tcagcttttc ctgttgctac taaagtttct
    42841 gctccacttt caggtggttg tactatttca acatccaaac cttcttcttt atagtaaccc
    42901 ttatcaagtg ctacaaataa tccagtatga tttgtatttg gtgtccaatc aagcaccata
    42961 gttacttttt tagaagaagt atctttatca ttgtttttgt ctttattgtt atttgaagaa
    43021 catccagtta ttgttgccaa tgtaaaaaca cttactaaac ctaatattcc taacttttta
    43081 aaattcataa ttgcctccct caaatacctt taatctatta atctatgtct tataacaact
    43141 ttttttacag cctgtacaat tcctacaaac attaaactaa atataactac taatatagta
    43201 gatgcaaata ctttatcaag tgcatatgca ctttttgacc taagcatata aacaccaagt
    43261 cctttcgttc cacctagcca ttctgcaaca gttgctgcca ttacagcata tgttgcagat
    43321 attttaagtc cagaaaaaaa tttatccatt gccataggaa atttaaggtg aatgaatgta
    43381 ttaactttat ttgagtccat agtcttaaat aaatttaaat aatctttatc aatattctca
    43441 atgccatcta caaaacttat taatatagga aaaaaacaag taagcattac catcaatact
    43501 tttggcattg ttccaaatcc aaaccatatc ataaagagtg gtgctataat tattgtaggt
    43561 atcatctgtg taaccaacat aataggataa agacattttt ttattattga tacaaaatcc
    43621 atcaatattc caattaccaa tgatattata attgcgacta taaaaccaag tattgcttct
    43681 cctatagtaa ccacagcatg catggataaa ttttgataat ctttaaaaaa cacattaatt
    43741 atatctactg gtgtgggtag tatatactga ggaacttcta aaactcctac catagattgc
    43801 caaagaataa gtataaacaa aaatgttgct attggatata tactatcttt gactttatct
    43861 atgatttcta agtttttcat ctatagtcag acctccagct ttttcatcta ctctagctac
    43921 tgttatgact ctttcacatc caagattaaa agggatttta tgtacctttt ttaataattc
    43981 aaaaacttca tcaaaatcac cttcaacagt agtgctcatt gctcctattt cacatttaag
    44041 accacttttt tcaatttcag caatacaagc atctactact ttatacatct cttcttcacc
    44101 tttgtaaggt cttagtggca ttactgctat gtctgcgatt accatttaaa tcactccttt
    44161 aaaaataatt aatacctact aggtatattt tgttttttat taaaaaattt tattactaaa
    44221 aagatattat atttaaataa aagaaaagtc ttatccaaaa taggataaga cttattttta
    44281 tcttaactgg tctccctaca ctggcattat ccaaacaggt tcaaagggtc gagataaaat
    44341 ctcctctcag cacattagct ccccgaaata tattatatct tttctacttg attatactac
    44401 acttatcata ttttaacaaa ttttttattt atattagata ttttatccgt tcaaaaatat
    44461 ctaatataaa ttgtacatta ttacaccaaa taaattatct taaaatggta ttttatcaac
    44521 atcttccaaa actagattaa ctgaattttc tagtttttat tttgctattt caagcatatt
    44581 tggatgtata tcaattccat acaaatattg tgctaaattt ctgttttcac tcatctaatt
    44641 tgcaatcgtt acagattaca gattagaaac agtttttaga ctctacttat ctttatctac
    44701 aaaaaatctt tcaataaatt cattaataat attcagtaat ctaaaaataa acctctatct
    44761 ggatttttaa gatacaaacc ttttaggaag tatcttttgt gataatataa aaattactcg
    44821 tcattatcat caaaatcctc agcaaccaac attttatata agaatcaaac tctataatat
    44881 aattatttta tccaatttat ttttgagaaa taaaatattt ttttaatttt taaatttttt
    44941 aatatgcacc ttgaaaaaag aaacgttttc ctatattatg gtaatagtag aataaaacta
    45001 aataggcaaa actagagaaa tttagtgacg caaagctata gggactaaga cttatgaaat
    45061 gagttatgtc agccagttgc caaagagata ttttcttttt gtttttatga atgtttttta
    45121 gggggatata tgatatatgt ttatggataa aatggataga tgtacacata tactaactgc
    45181 atatatatgc agttcacgcg attactgcaa ttttatagat acacaattaa atgattttat
    45241 actagaatat ggagagaatg tggtagaatc ttgtttgcat caagtgatgg tattggtaag
    45301 cagatataat taaagaaatt ttattttcta gaaaaccctt tttagtttta tctacgtttt
    45361 ataattcatt aaagaactaa ttaaaataag cttcattata tatttttcat gaagtaaaca
    45421 gttaagttta tctcatatac tgtttacccc aattacataa ttctaataat atagattcca
    45481 atgatttacc acgtttagta gacaaatatt caacttttgg aggaatagtc ggataagaaa
    45541 tcctcttaat aagtccatct ctttcaagat actttaattc ttgtgacagt atcttatgac
    45601 ttatatgaac aacttctttt tttaattgac tgtaacgtat tggctcttgt tttaaaatat
    45661 gccataaaat aattgccctc catttacttc ctatcacagt catggtaaaa ccaattgaac
    45721 aattaaaatc tttttgtctt tcttcaatat ttggaaattc ttcaaaatta ttatctatat
    45781 ccatctcaat cctctctttc aaaatagtaa gtatctaact aaaaagtgca ttcttgttta
    45841 gcgtactaat tcgtaatata atcttgatta aaaaatagct atgcacactc gtgactttag
    45901 tcatgggagg ttcagtgtag catagagcct tacataatac tactttttta gatgtatatg
    45961 tttaaatttt aatatagaca tatgttattt tattaatttt atttaaacag ataataataa
    46021 ataataattt atctcaagtt ttctatgtag ataaactaat ctaatgttta gaaaggatta
    46081 tataatatga ataaaaaatt tcgtctttat caagttgact cattacaaaa gaaaaattta
    46141 caggtaatcc agctggtgta attacaaatg ctgatggctt atctgaatca caaatgcaaa
    46201 aaatagcaag agaattaaat aattcagaaa ctgcttttat tttcaataac ttggctaaaa
    46261 atcatcaaga aaattatgat gtacatatca gattttttac accaactaat gaggttccta
    46321 tatgtggtca tgctactatt gctgcccatt atgttagagc aatagaaaac caattcaata
    46381 acaaaacagt atttcaaaaa actggtgctg gaatattacc agttgatatt ataaaaaaag
    46441 ataatgatta tgaaataatt atgacacaag gaaaaattga agtatctcca ccattacaac
    46501 aaacctatca aaaaagatta cttaatgcat ttggtattta aaggaagata ttcgtacaga
    46561 ttgtcctatt gccatatcat ctacaggaca ttcaaaagtt atgattggaa taaaggaatt
    46621 atcaacatta aatagtttat ctccaaattt atatgaatta tcgaaaataa gtcaagaaat
    46681 aaaatgtaat ggatattata tttttacatt acataataaa gaaactctac aagttcatgg
    46741 aagaatgttt gctcctgcaa ttggtatttc agaagaccct gttacaggaa atgctaatgg
    46801 acccttaggt gcgtaccttg tacattataa agtaatacct acacccaaca cagagtttat
    46861 atttgaagca gttcaaggag aagcaataaa acgcaaaggc aagatgttag taaaagtaat
    46921 tattttagat aataagccag ttgaagtaaa aataattgga aaagctgtaa ttgcattctc
    46981 aacaaacata gaaatctaat tatagattcc ttctaagtaa atttttgaac tttttaatac
    47041 taatttaata acaaactaat ttatatacaa tcaaattaat ttttttatta tttgttaata
    47101 tatatt
//
LOCUS       DAMJVK010000021        46109 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.021, whole genome shotgun sequence.
ACCESSION   DAMJVK010000021 DAMJVK010000000
VERSION     DAMJVK010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 46109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46109
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.021"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            161..544
                     /locus_tag="PC414_001163"
     CDS             161..544
                     /locus_tag="PC414_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDF2463569.1"
                     /translation="MNLSKKIEDKRKAKQRAEKVKKAKIATAGVVLGAVTGAVSGVLL
                     APKSGKETREDIKDASQQIAEKINMKTVDVKGKVSEKLEDKKGNFIESKKKIKKYIDN
                     KKSVENSSEEDKIIDIVEPVSVTEE"
     gene            546..896
                     /locus_tag="PC414_001164"
     CDS             546..896
                     /locus_tag="PC414_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463570.1"
                     /translation="MDNTITINISIIYVLLAILACIALVYLIIVLVKLHKILNNVGTM
                     LNENTQNINTTLNNLPNVVETFGEVGENMKDVTEVVTEVTADFLVTKDSVKSNMDVIV
                     DILTIIKNIFLDKK"
     gene            complement(997..1455)
                     /locus_tag="PC414_001165"
     CDS             complement(997..1455)
                     /locus_tag="PC414_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463571.1"
                     /translation="MIRTKVVELIATVCRENKPHKWVDENYTPYDKSGKVELMSIEDL
                     NELISSSGRADFLYSSRLQKLLEEVYINQSRASYISGCGLFWSSYWDILEEKFEEWLY
                     NSYIFFDEDDEYLEGMEDFELECKDVLMDVIETTSIDIYVQMIKRNITNY"
     gene            complement(1500..2288)
                     /locus_tag="PC414_001166"
     CDS             complement(1500..2288)
                     /locus_tag="PC414_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2463572.1"
                     /translation="MKVVIIPGHTLIGKGTGAVGYINESKETRILNDLIVKWLKIGGA
                     TVYTGRVDESSNHLADQCAIANKQETDLAVQIHFNSNATTSTPVGTETIYKTNNGKTY
                     AERVNTRLATVFKDRGAKSDVRGLYWLNHTIAPAILIEVCFVDSKADTDYYVNNKDKV
                     AKLIAEGILNKSISNSQEGGENKVYENVIVYTGDADKVAAQILHWQLKDSLIIEASSY
                     KQGLGKKVYVVGGEANKLVKGDVVINGADRYETVKLALQEIDKL"
     gene            complement(2427..2864)
                     /locus_tag="PC414_001167"
     CDS             complement(2427..2864)
                     /locus_tag="PC414_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HDF2463573.1"
                     /translation="MNKKLENRCSWQEYFMRLCETVAERGTCDRAYVGAIIVNSENRI
                     VSTGYNGSISGDKHCSEVGHEMRDGHCIRTIHAEQNALYYCAKEGISVKNCSIYVTHF
                     PCLNCTKAIIQAGIKHIYYRTGYRIDEYAIKLLQSSNVLYTKL"
     gene            3686..4195
                     /locus_tag="PC414_001168"
     CDS             3686..4195
                     /locus_tag="PC414_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2463574.1"
                     /translation="MLSENIKTIRKSKGLSQQELAIKLNVVRQTISKWEQGLSVPDSD
                     MLISISEVLETPVSTLLGETIVESEVDNLKVISEKLEIINLQLAQRKNMRRKMFHWLF
                     ILLCAVILIVFVVLGGLNSPYLDWNYTDPETAVFGVAFHAFEWLFVRLVPIVLIGAII
                     GIFLTRKKI"
     gene            4601..5137
                     /locus_tag="PC414_001169"
     CDS             4601..5137
                     /locus_tag="PC414_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDF2463575.1"
                     /translation="MQGGVNIRQVKSFVFPVISLIFLDQISKVLIGLFLMDFEIDIIG
                     KFLRFNPVQNTNLSYGGNFIGILSNLWVLVLFNILVILVIISGYAFYKSKNEQTSYSV
                     KVIMSCGLAGTICSLIDKLFWGGSLDFLQIPSFFIFDLKDCYLTVAEIIFVVIGILHN
                     REISMKEYIYFCYRQFKK"
     gene            complement(5564..6364)
                     /locus_tag="PC414_001170"
     CDS             complement(5564..6364)
                     /locus_tag="PC414_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2463576.1"
                     /translation="MRLNKRSEGYINYVKGVKREKRNVLFYQLLILIGFIVIWELLAD
                     LNVINTFLFSKPSDIYNLFIQYAFSGQLFKHIGISVYETVLGLVIGTVLGILVAIALW
                     WSEKLSKILDPFLVVLNALPKTALAPIIIVWVGAGIEGIVVTAVTISVVVTILSAYNY
                     FMNIDEEKIKMLKSFGATKSQILFKLILPANTGNLINLTKINIGMAWVGVIVGEFLVS
                     RYGIGYLIVYGSQVFKLDLVMMGVFVLAICAWAMYAVVNIIEKIYNSR"
     gene            complement(6559..8157)
                     /locus_tag="PC414_001171"
     CDS             complement(6559..8157)
                     /locus_tag="PC414_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF2463577.1"
                     /translation="MKAAIYSRKSRFTGKGDSIENQIQLCIDYAKSIGVKDFLIYEDE
                     GFSGSNIDRPQFKKMMQDAKDKKFSYLVCYRLDRISRNVSDFSTLIEKLNELSISFIS
                     IKEQFDTSTPMGRAMMFISSVFAQLERETIAERIKDNMYELARSGRWLGGNTPHGFNS
                     EKITFLDENLKERSMYKLQVNDEQMEIVKLIYNKYLELRSLSKLYKYVYNNGIRGPRG
                     GKFDPSSLSRILRNPAYVKANDEILDYLKNSGMDVVGTPDRKCGILTYAKNTSNSIAA
                     IAKHHGVIEPEIWLEVQNQLDKNRDKTPRISTGKTALLSGLIKCGKCGSNMRITYKGK
                     KTDKDLKYYYICGTKKTLGSDSCNCKNLNGPLVESLVIDKIKNCKEESIISAFNSNNI
                     KTDNVFNIFKSETNLLKNQILDKERLIKNLVIELAKNTGSTASDYIVSQIESLNNEIS
                     ILKDKINTLESSNINISSDSINIDIIVSNLKRFNDEVDNASLDDKRLLLSTIINSIVW
                     DSSLGNLNIVYMGAEVDNSVSLSN"
     gene            8485..8661
                     /locus_tag="PC414_001172"
     CDS             8485..8661
                     /locus_tag="PC414_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463578.1"
                     /translation="MEINVIEIFPKDKAKLNKIEMDKASWFVNIISKKYPKEALNEAF
                     STLEKELNISKANT"
     gene            8835..9965
                     /locus_tag="PC414_001173"
     CDS             8835..9965
                     /locus_tag="PC414_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HDF2463579.1"
                     /translation="MNNFEIKTRIKFGEGSLKALQELKNKNVLIITDPFMVKSKTIDK
                     ITSNLINSTYQVFEEIVPDPPIEVVVAGIEAFKSINPDVIIALGGGSAIDAAKSIMDF
                     SKKILKINEVEFIAIPTTSGTGSEVTSFAVITDSQKKCKYPLVSDDLLPDMAILDPEL
                     VKTVPNFITADTGMDVLTHAIEAYVSTNSNDFSDALAEKAIKLVFKYLIKAYKDGNDI
                     EAREKMHNASCLAGMAFNQASLGINHSIAHVLGGKFHIPHGRTNSILLPHVIEYNSSI
                     VGYTNTTYSATAKKYAQIAELVGLGSANIRISVRNLVNEIKKLQKEMNMPTKLTQCKL
                     SLEDITNEEREIAKLAIKDTCTLTNPKVPTEDDIINILKNIK"
     gene            10206..10556
                     /locus_tag="PC414_001174"
     CDS             10206..10556
                     /locus_tag="PC414_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HDF2463580.1"
                     /translation="MTEESKQRVIQEYVPGKQVTLAHIIANPNEDIYKKLGLVLDKKD
                     AIGILTITPSEASIIAADVATKASNVSLGFIDRFSGSVVISGDVSSVESALNDVLEVL
                     GNMLNFSSTKITRT"
     gene            10564..11013
                     /locus_tag="PC414_001175"
     CDS             10564..11013
                     /locus_tag="PC414_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HDF2463581.1"
                     /translation="MKNIIFMGKTGSGKTTLCQKLDQLELKYKKTQSVELYNNAIDTP
                     GEYMENRNLYNALITTSADAKIIAIVYDATQEENYIAPGFASMFCKDVIGIITKINKA
                     TENEVNIAYERLSLAGASQIFKIDTVDNIGLEKLFKYLEKYKNIDEV"
     gene            11115..11690
                     /locus_tag="PC414_001176"
     CDS             11115..11690
                     /locus_tag="PC414_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDF2463582.1"
                     /translation="MGKKILLVDDEPLLRMDTKDILESHGYEIIGEASDGFEAVEMCK
                     KHKPDLVIMDIEMPILDGIKAGKIICKENIAGGVLLLTSFEDKEYVEKAKSIGAYGYL
                     VKTASEKSLIPTIEMCLNKVNEFEKMKKDLEKVNSKLNERKLVERAKGILIREFKISE
                     DEAYTRIRKLSMDKRTTMMEIAKMIIIGYDD"
     gene            11683..13092
                     /locus_tag="PC414_001177"
     CDS             11683..13092
                     /locus_tag="PC414_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal domain-containing
                     protein"
                     /protein_id="HDF2463583.1"
                     /translation="MTNTIKKISTAQTYLNNNEIEKIIEVSKSLDLMASFYEADVFID
                     VLSKFDDEAIVVAHGRPKEKSMYCENVIGKKALIENEPGVIKCLKTGERVRDIKALTQ
                     EYKLVKQNIQPITLDDKVLAVLIVEKDISSEVKNEFNASEDEENKNLHQFIKLIGNND
                     FLVDNLNSAILIFDKNGKLKIKNRIATEIYKNLGFGYDIQDMHYNDLDIETNTFENIL
                     MDENYDETNEVSIGNMFFKIKTIYIKDDEFKVCKIVQDISDLKRKEEELVLKTVAIRE
                     AHHRVKNNLHTVISIIKKQSRLSQNDEVKQCLDNIINRVFAILSTHYLLSKEVDNNIS
                     IREGINLLVSNIQGGYLFSKDIDIRITGEDFKISGEKATAILLVINEILQNCYDHAFS
                     DREHGTIQILINKDNDYRNIAIIDDGIGFDSNNINKNSLGTYIIDSYITQMLKGNIER
                     KSSENGTEVLITIPTQINI"
     gene            13276..14709
                     /gene="eutA"
                     /locus_tag="PC414_001178"
     CDS             13276..14709
                     /gene="eutA"
                     /locus_tag="PC414_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HDF2463584.1"
                     /translation="MREELLSVGIDIGTSTTQLIFSKLIVENTESNFSVPRISIVDKE
                     IIYKSKVYFTPLISNNEINGQQIKDIVENEYKKANISKEDIHTGAVIITGETARKENA
                     NDVLHTLSGFAGDFVVATAGPDLESIISGKGAGAHIYSKEHSTTIVNIDIGGGTSNIA
                     SFKRGEVTDTGCLDIGGRLVKIDKISKKITYISPKIDKIIKDNNLNLQVGTMATVENT
                     KPIVDIMVDLLMESIGLKKQTPLFDFIITNKSISIDENIKNVSFSGGVADYIYYDGEI
                     KDYFEYGDLGILLGQAIKNCEYLKKVNVVKSMETIRATVVGAGSHTTEVSGSTITYTS
                     EKFPLKNLPVLKLSIDEETGTSKQISEAIKNKLNWFKLENDLQKVAIAMIGKKNPTFL
                     DIQEYAKGFVDGMQQIIENDLPFIVIVENDMAKVLGQAIYALLNYEKEIVCIDSVKVE
                     NGDYIDIGKPIVEGKVLPVVIKTLVFN"
     gene            14727..16091
                     /locus_tag="PC414_001179"
     CDS             14727..16091
                     /locus_tag="PC414_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HDF2463585.1"
                     /translation="MILNTKLFGRTYQFKGLYDVMAKANEEKSGDKLAGLAAESAEER
                     IAAKVVLSHIKLEDIRNNPAVAYEEDEVTRIIQDAVNEKVYEEIKNWTVSELREWLLD
                     SNTTSLDIRRISRGLTSEMIAAVAKLMSNMDLVYAAKKIKVTAHCNTTIGEQGTLSAR
                     LQPNHPTDDPDGILASLLEGLTFGIGDAVLGLNPVDDSVESVTKVLKRFEEVKQKYQI
                     PTQTCVLAHVTTQMEAIRNGAPADLIFQSIAGSEKGNEAFGFNGDTIEEARQLALKHG
                     TATGPNVMYFETGQGSELSSEAHHGIDQVTMEARCYGFAKKYEPFLVNTVVGFIGPEY
                     LYDAREVVRAGLEDHFMGKLHGLPMGVDVCYTNHMKADQNDMENLAILLTTAGCTYFM
                     GIPHGDDVMLNYQTTGYHETASLREMFGLTAIKEFDAWMEKMGFSKDGKLTELAGDAS
                     VLLG"
     gene            16104..16985
                     /gene="eutC"
                     /locus_tag="PC414_001180"
     CDS             16104..16985
                     /gene="eutC"
                     /locus_tag="PC414_001180"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014952556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HDF2463586.1"
                     /translation="MNEKDLKALVEQLVGQMVGELDTNVVSETVKKATEVVVDNNACI
                     DDITEVDIRKQLLVKNPKDAEAYLDMKAKTPARLGIGRAGTRYKTETVLRFRADHAAA
                     QDAVFSYVDEEFIKENNMFAVETLCKDKDEYLTRPDLGRKFSPETINNIKSKFGTNQK
                     VLILVGDGLSSAAIEANLKDCVPAIKQGLKMYGIDSSEILFVKHCRVGAMDHLGEELG
                     CEVICMLVGERPGLVTAESMSAYIAYKPYIGMAEAKRTVISNIHKGGTTAVEAGAHIA
                     ELIKTMLDKKASGIDLK"
     gene            17005..17658
                     /gene="eutL"
                     /locus_tag="PC414_001181"
     CDS             17005..17658
                     /gene="eutL"
                     /locus_tag="PC414_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HDF2463587.1"
                     /translation="MKNDVIRPNILGIKIISNISPEMAQKLELKSHHKSLGLITADCD
                     DVTYTALDEATKASEVDVVYAKSMYAGAANASTKLAGEVLGIIAGPSPAEVRSGLNAV
                     VDFLEYGATFISANDDDSIAYYAHCVSRTGTYLSEVAGIREGEALAYLVAPPLEAMYA
                     LDAAMKAADVKMCELFAPPTETNFGGALLTGSQSACKAACDAFAEAVKSVADNPTGF"
     gene            17669..18370
                     /locus_tag="PC414_001182"
     CDS             17669..18370
                     /locus_tag="PC414_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HDF2463588.1"
                     /translation="MDAIGLIEVIGYVAAIEASDACLKSANVNIVRVDKVGAGIVTLT
                     ICGDVGAVKAALEAGEIAASRVGTLRTSHIIPRIHNEVTNALFKTKEPKVCEVSEDKE
                     KNFEITLEIADNVSKNLSEKENVILDVNENSGDTENLYSDIDKTNKKIETVDDSLTEA
                     NEEKIENTNDKEVLEQVKIENSDFDRDDLSKNSVKKLKAMAKKLDSSITYKDLNVLKK
                     EELINLVKKLSRRDR"
     gene            18373..19842
                     /locus_tag="PC414_001183"
     CDS             18373..19842
                     /locus_tag="PC414_001183"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HDF2463589.1"
                     /translation="MTLLDKDLVSIQEVRTLVRRSKVAQEKLSKMSQEQIDKIVKAVV
                     DAAFNNATKLAKMANEETGFGKWEDKVIKNTFASMGVYETLKNTKTIGIIGEDKEANT
                     IDIATPVGVVAGLIPSTNPTSTVIYKTIISLKAGNTIVFSPHPNAKNCILETVNIISD
                     AAIKAGAPEDSILCITNPSIVGTNELMKHKDTNLILATGGEAMVRAAYSSGTPAIGVG
                     PGNGPAFIDKSANVKLAIKRIIDSKTFDNGVICASEQSIVIEKSMEETVLKELKNQGA
                     YVLNEEESERLAKFILRANGTMNPQIVGKSVEVIANLADLTNVPKNTKVLVARENKVG
                     HNVAYSREKLTPILAFYVEENVEAIMNRCREILLNEGAGHTFSMHVNDEELVKRFALN
                     MPVSRILVNTPSALGGIGASTNLVPALTLGCGAIGGSSTSNNIGPLDLINVKRVAYGI
                     KEIEELGRDKSQVEKSMFDSMDKEELINLLVQKLVKELA"
     gene            19938..20225
                     /gene="eutM"
                     /locus_tag="PC414_001184"
     CDS             19938..20225
                     /gene="eutM"
                     /locus_tag="PC414_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HDF2463590.1"
                     /translation="MASANALGMIETKGLVGAIEAADAMVKAANVQLVGKEQVGGGLV
                     TVMVRGDVGAVKAATDAGAAAAERVGELISVHVIPRPHFEVDAILPKVSAE"
     gene            20352..21113
                     /locus_tag="PC414_001185"
     CDS             20352..21113
                     /locus_tag="PC414_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin adenosyltransferase"
                     /protein_id="HDF2463591.1"
                     /translation="MGVLTENEVKRKIGKNKNNITEFYIEKGQIITPSAKSYLAERSI
                     ELKYVDKLSDVNNEPKEVVKEVVKEKKYRYTTVFGAKLDEKPEHMTQLNGNLLVFKDH
                     KRIILRGKIDSLESEILKVQILCQKLDMPKLVKELQEVLDFVRNIMRCEVLDEKLGDF
                     KLLGLTPQELREQSHYPKKYFGIGHEFPSYEMGEVVVAINSIRSNTREVELAAYEAFK
                     GEYGNVHREDLIKALNRLSSVFWIMIYKVRTNKYK"
     gene            21126..21755
                     /locus_tag="PC414_001186"
     CDS             21126..21755
                     /locus_tag="PC414_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HDF2463592.1"
                     /translation="MENLLNEIVEEVITRVKKEAFIEVEASGRHVHLSREDVDKLFGE
                     GYTLTKLKDLSQPGQYACKERVTITGPKGSIKNVVVLGPCRNETQVEISLTDGSTLGL
                     KAPIKQSGDLEGTLSIKISTQYGEVELDKGLMVAKRHIHMTPKDAEKFNVCDKEIVEA
                     KVMGQRPLTFDDVVIRVSDSFKTYMHIDYDEANACGYSKGTVAKIIKKA"
     gene            21797..22522
                     /locus_tag="PC414_001187"
     CDS             21797..22522
                     /locus_tag="PC414_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02536 family ethanolamine utilization
                     protein"
                     /protein_id="HDF2463593.1"
                     /translation="MNYDNLVDLITEEIYKKINSNEIKISNKPKAVIVFEQDNNKFNL
                     LKDEFEIVEFDKSIRECEIVIVSRLCMRGLSNIALGNSTSDEERFILKMIMKGKEVYV
                     LDEGIEYKKYKDTAPKALYKKFMSFEDEICKFGVEIIKDLNVITKNKLNIKKELESTR
                     GSKKEDTKVLDKDVLNLTSVESVKIDNEFSLDLRNKKLISEADLRKPTINGVKNILVN
                     KKSIITPLAVDFMRIHHLKLKKV"
     gene            22535..22807
                     /locus_tag="PC414_001188"
     CDS             22535..22807
                     /locus_tag="PC414_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HDF2463594.1"
                     /translation="MDIGKVVGNVWATRKDEKLCGQKLLVVKILKTKDTYKEGFMVAA
                     DNAGAGNGDLVLISKGSSARESIEKSHVPVDATIVGVVDSFEVFDE"
     gene            22800..23351
                     /locus_tag="PC414_001189"
     CDS             22800..23351
                     /locus_tag="PC414_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HDF2463595.1"
                     /translation="MNKSIGAIEFKSIAKGIEVSNEMIKKSSVDVLYLKSICPGKFLI
                     IVGGETSYINECVDYGIKLGEGYIVDNFVINAISQEILDGFKNKYQKLDSIVSIGVVE
                     SSKVCAGIKMLDKTLKSGDLVLVKLQLSFAIGGKLVYIVAGDLSSLEYALKESENVVR
                     EKEVIYKTVIPSVDSQIIKSLIK"
     gene            23363..24454
                     /gene="eutH"
                     /locus_tag="PC414_001190"
     CDS             23363..24454
                     /gene="eutH"
                     /locus_tag="PC414_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HDF2463596.1"
                     /translation="MSINEIIIYVMVVFMVLGAIDKCIGNKFGLGEQFEEGIMAMGSL
                     AVAMVGVICLAPVLADVLRPIVVPVFNILGADPAMFAGSLLANDMGGAPLALELAKDP
                     NAGLFGGLIVGAMMGPTIVFIIPVALGIIEKEDQKFLATGILAGIITIPIGAFVGGLV
                     AGFEVMMVLRNLVPIIIFAVVIALGLLKFENAMIKGFTYFGKGVVIVITLGLAAAIVE
                     ALTGIVVIPGMKPISEGIEIVGDIAIVLAGAFPLVFVITKVFNKPLMALGKVLGMNDV
                     SAAGLVASLANCIPMFGMMKDMDNRGKIINVAFSVSAAFVFGDHLGFTAGFNSNMITP
                     MIVAKLVGGITAVLLAMVIANKTLKKENV"
     gene            24456..24929
                     /locus_tag="PC414_001191"
     CDS             24456..24929
                     /locus_tag="PC414_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF861 domain-containing protein"
                     /protein_id="HDF2463597.1"
                     /translation="MDISNIDKNLIETLVRQIIEEKISGTKDTVDFVRNKDISGITSI
                     KLPTVKVSESDRLDTGNPSDVVYTKDLFTLEESPRLGCGMMEMKETTFDWTLNYDEID
                     YVIDGTLDIIIDGRKVSASSGELIFIPKGSKIQFSVPDYARFIYVTYPADWASQN"
     gene            complement(25049..25150)
                     /locus_tag="PC414_001192"
                     /pseudo
     CDS             complement(25049..25150)
                     /locus_tag="PC414_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433047.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            25445..25966
                     /locus_tag="PC414_001193"
     CDS             25445..25966
                     /locus_tag="PC414_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2463598.1"
                     /translation="MEEIVKKEQASMNDIFLYLKAKSNSLYKFVILYNEFINKPKDYG
                     TGELINMVEVHMLTAIEENPGISPTQLAKIWNRTKGAISQTISKLEKKGYITKEKKEG
                     NAKTILLYPTTSGVELSKAHKLYDTIDVTRTFEYLIKECTLEEIDSFYKVIDLFINLL
                     EESPQENNDSEKV"
     gene            26345..27520
                     /locus_tag="PC414_001194"
     CDS             26345..27520
                     /locus_tag="PC414_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2463599.1"
                     /translation="MNLKQLSEKYEEYVIQMRREFHKYPELSFKEIRTTQRIREELQK
                     IGVPFVSIEPNIVIATIQGKNEGKTIAIRGDIDALPMQEDSDVEYKSEYDGIMHSCGH
                     DAHGAMLLGLAHMLNDIRKDLKGRVLLVFQAAEEVGGGHKEIIQYFKENGGPDRLIAT
                     HVWSDIEAGKISVEPGPRMAGGSGWRIDITGRGGHGSRPDQSIDPIKPACDIVLKVSS
                     IPVNHISVLEQCVVHACAIHGGTTIGNVIPNNAEVIGGYRYFTEESGKKVFDIIKQMS
                     NGVGQVYGVDVKVTHTGGIGPVINDEEAVKMAKEIVSNIDGLELDSYEPICASDCYGE
                     ILKEYSGFYCYLGVGNKEKGIIYAQHHPKYNIDEDTLKLGCEFMAKYAVEFLNKNNN"
     gene            27555..29093
                     /locus_tag="PC414_001195"
     CDS             27555..29093
                     /locus_tag="PC414_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HDF2463600.1"
                     /translation="MQGALKENKKKSGGGFLGWIEKVGNKMPHPLALFTYITLIVVAL
                     SAIASFLGFSATSPATGKLIKVMNLISAKGLVLFMQEFVKNFQNFPVLGVVLVMAVAT
                     GVCEKTGFFSTAIKMSLSKVKGNAVVFIIAFIGVFANQAGDAAFVLVPTLAGAIFYGL
                     NRNPLAGIFCGFASVGGGFATAVIPGGWDVTLTPITVSAAQTIQPAFDMTLLASYYAL
                     FVSAFIVATVSTIVTVKFIEPKLGKYTGKPEGIDDNQGFEVTKEQAKAAKLAGFTVLA
                     YVALLIALCIPANSFLRSPEGSLIFGAPLMSCLQFFIVILFFLPGIVYGMRVGKIKTV
                     KDLNDILCQSMAAMAPFIVLAIVIGQFLTLFSVSNLAQVLAIKGGALLNSLPLPPQAI
                     ILLFLVLVAFINIFVISGSTKWMIFGPVFVPMLMQLNIHPAFTQFVYRLGDSVTNHLS
                     PLNAFFIILLATVQKYDKNAGMGTIFSAMIPYTIGYFVVLAVLVVVWMTIGIPVGIGG
                     QVWL"
     gene            29125..30327
                     /locus_tag="PC414_001196"
     CDS             29125..30327
                     /locus_tag="PC414_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2463601.1"
                     /translation="METTEIRYNFDEVIDRSGTNCLKYDLKSQFMPESPEDALPLWVA
                     DMDFACAPEIINAMHERIDRKIFGYSSQQTDDYYEAVCGWFKRRFDWKIEKENIFFSP
                     GVVPALGFFTQILTQKGDGIIIEKPVYYPFEDKITNNERKVVNSPLKFEDGHYSMDYD
                     GFEELAKDENNKVFILCSPHNPVGRVWKKEELRKIVDICKKYDLWIISDEIHCDIIRK
                     GEKHIPLEVACPDYKDRIITCTAPSKSFNLAGMQLSNIVINNKELQKKWMLETDSKLA
                     IFLPNPFAIVATKVAYNDCEDWMNQVNEYIDGNIKYVEDFVNEYMPKVKIIKSQGTYL
                     VWLDFRGYGLDAKQLEYVMLNKAKVLLDEGYIFGEEGAGFERINVASPRTIIEECMNR
                     IKCAFEGL"
     gene            complement(30494..31093)
                     /locus_tag="PC414_001197"
     CDS             complement(30494..31093)
                     /locus_tag="PC414_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2463602.1"
                     /translation="MNDNIVEVKNINMNFYTKEGELEVLKDVNFNLKEGEILTLLGPS
                     GSGKSTILNILTNLLKPTSGDIKITGNIGYMFQKDNLLEWRNIMDNITIGLEIQGKKD
                     KKSLDRVESLLKTYGLWDFRSMYPKELSGGMRQRVALIRTLSVNPDILLLDEPFSALD
                     YQTRLLVCDDVYSIIKNENKSTILVTHDIGEARCIKVQL"
     gene            31263..32621
                     /locus_tag="PC414_001198"
     CDS             31263..32621
                     /locus_tag="PC414_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463603.1"
                     /translation="MSNIITNLIESIFTNPITILFLLAITVYSVFKIIENNRVYSFVS
                     SKLDEINRDYKTSEFYKKVRDDYYTYSKENPYADVNITSFIEEVVSDLKHNNLPLLEK
                     IRSIKNSSSISILLGVLGTFVGLSTMLLCVDTKDIINSLPSTISSMQTAFTTSIFGVV
                     FSLIIGYFTKIKDCEHVLIQIMLKSENLLTSEITHFKSERMDLKVEEVKNTIKQISKS
                     IEAIEKFDKISKDLNDFNDEFISGIEALKSLLEGSQSSIKTFDQSVRKLDKQFNILNI
                     KFVKLFDKYDNQDNINKEILFDIKESSKNIYNATESQFKIRDYIKNINAGFALYERSA
                     QDLLTKLMTHENKISQNQKILLDEKFTLDDSIKNLSSIIENFSNDLQVKLDMMFENSL
                     DIQDKLDVMFNNSFMNDEVPLDSEELFKDDVNNVFNPFSEEIYEIEDKEIKVIGEDEL
                     NE"
     gene            32614..33246
                     /locus_tag="PC414_001199"
     CDS             32614..33246
                     /locus_tag="PC414_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDF2463604.1"
                     /translation="MNNKYRRTVNREFEKSSFWPTFTDLLSTVLMVVILILFSSESIS
                     GSVEQDLAKNVNASVEETFKKSGIPVKVDKSNGQVTFGEKTMFDVDSDVLKPEAKEML
                     KMFVPKYIETIYKDYGDYISKIVIEGHTDDVGSYIYNLDLSQRRAYSVAKFIVGDEIG
                     DYKYKDKVTKHIIAIGRSKAELIKNGDNSVNRDASRRVELKYEININQNK"
     gene            33575..34732
                     /locus_tag="PC414_001200"
     CDS             33575..34732
                     /locus_tag="PC414_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2463605.1"
                     /translation="MIEISIKNKKNSKIKTLILLLIVSISIYLTGCTTSDNGNNQSNN
                     NDSLSSSISSDKNVKIHFIDTGNSDAILIQDGKTFTLIDGGDNDDEGLMVDYLNNQGV
                     KDIKYLIATHSHADHLGGLDSVVKNFNIENVFVSNGSAETKSYRDFINALANKGLSPS
                     VPLENNKFYLEDSYFEVLNTNGGDTTNEQSLVLVYTNGNDKVLFTGDAEEGTEKEILP
                     KLEKVDLLKVAHHGSRSSSSQEFLDKVNPEYAVLLVGKGNSYGHPHQETMNKLKKMGV
                     KVHRSDECSDIIFESTGDGVFTSCKDGSYNKGVREDGNSGKSNSNTSKNDSFKNKQNT
                     SNSEEIVYFTPKGKSYHSTKNCSGLSRSKKILSGTIAESKKNDPCDICYGK"
     gene            complement(34879..35514)
                     /gene="lexA"
                     /locus_tag="PC414_001201"
     CDS             complement(34879..35514)
                     /gene="lexA"
                     /locus_tag="PC414_001201"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HDF2463606.1"
                     /translation="MYLDLTEKQVLILEFIKSQIILKGYPPAVREICTAVGLRSTSTV
                     HSHLNKLEKLGYIRKDPTKPRAIEVLERSKVNDVSGANQEIIELPLVGQITAGEPILA
                     QQNIEEYIPFPASLVKGSNNFVLRVKGESMINAGILDEDYVVVDKKNTALNSQIVVAL
                     INGESATVKRFFKEGNLIRLQPENDFMEPIMLNDSEVEIVGIVTGVFRVIK"
     gene            complement(35618..36745)
                     /locus_tag="PC414_001202"
     CDS             complement(35618..36745)
                     /locus_tag="PC414_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF2463607.1"
                     /translation="MNNDKKIIKVHNKSDKPDNIVIKENELIEKCLLLENKLPVFMKD
                     YFIYLKGSVAVSTRLAYLEDINFFCSYLIETKELTNADCVKDITEDDFNTIKSRDINL
                     FLGDYCSRYYKNTEKNTLIFENNNRALARKKSSISTLFKFLYRNSQIDNNITDGFNPI
                     KLPKPQPDAIKRLEIDEVAKMLESVETGEGLTEKEKVYWRKTKLRDKAILALFVTYGL
                     RLNELRELNISSFNFSRGEFKIYRKRGKEVLMPINHTCEHVIKDYLQNERTRDELLND
                     EEKDALFLSLQNKRITAKAIRTLVKKYTSIPLDTTRDNGYSPHKLRATAATSLIQTGF
                     SIYDVQNLLDHDNVTTTQLYAAHKKNVKRDIVKNFEWIEDD"
     gene            complement(37056..37901)
                     /locus_tag="PC414_001203"
     CDS             complement(37056..37901)
                     /locus_tag="PC414_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463608.1"
                     /translation="MIYIIEILKISVINIFCFIGIFIVFGLIFSITENLNNKFIFSSF
                     GKTGIIVTGAIGTFVHELSHLIMCLIFMHKINSVKFFRPIESKNDGILGYVSHSYKKD
                     NLYQSIGNFFIGIAPIIGGTIMIILIFKILLPDSYMKVTQNIDLKLYVSMINNLNING
                     FIHIILGDISNFIRIMLLTPSVYSLKYFIFMFLMYSISTHMSLSSADLKNSLNGLSFI
                     VIVIFVISSITYLFGFDNLNISSLIVKYNIFISFFMAIGFIFSIVTLIISYILSLVSP
                     FKNFS"
     gene            complement(37931..38302)
                     /locus_tag="PC414_001204"
     CDS             complement(37931..38302)
                     /locus_tag="PC414_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HDF2463609.1"
                     /translation="MKLKLLVEEYAVCRLNNDSKIPTWIDTEKFYSITRTDDELSVVC
                     LNNNIPSDVKSEKEWRILKILGPLDFSLVGILSKLSSLLADNKISIFAISTYDTDYIL
                     VKEKDIENACKILSCNGYEVE"
     gene            complement(38455..38715)
                     /locus_tag="PC414_001205"
     CDS             complement(38455..38715)
                     /locus_tag="PC414_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HDF2463610.1"
                     /translation="MEVLKVSSKSNPNSVAGALAGVLRERGVAEIQAIGAGALNQAIK
                     SIAIARGFVAPSGMDLVCIPAFTDIEIEGDKKTAIKLIIEPR"
     gene            complement(38904..39848)
                     /locus_tag="PC414_001206"
     CDS             complement(38904..39848)
                     /locus_tag="PC414_001206"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HDF2463611.1"
                     /translation="MIENNSEKIKVLENDIKQLITISKANNIDLSDKINSLNEKLEKL
                     KEDAFSHLSAYEKVTLSRDIKRPTTLEYIEHICSNFLELHGDRLFKDDPSMVGGIGQI
                     GKFNVTIVGHQKGRDTKENIKRNFGMPHPEGYRKALRLMKQAEKFNRPIITFIDTSGA
                     FCGLEAEERGQGEAIARNLLEMSKLSVPVITFVIGEGGSGGALGIGVGNDVCMLEHSV
                     YSVISPEGLSSILFKDSSKAKEACDVMKLTSNDLYDLKIIDKIIKEPLGGAQKDVEAV
                     SKEIKAYILERLNHYKDMDKEEIIAQRYKKFRNIGKCL"
     gene            complement(39869..40723)
                     /locus_tag="PC414_001207"
     CDS             complement(39869..40723)
                     /locus_tag="PC414_001207"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HDF2463612.1"
                     /translation="MIKKFLSSKESKYVTISLDDNFKKNSVDDKFWTYCKGCDSHVFR
                     KDIEENSFVCPKCSRHYGLRARKRINLLIDKGTFMEFNSDVEFQNPLNFPKYKEKVDS
                     YKEKTKESEAVVTGYGRINGIKTVICVMNPDFMMGSMGSIVGEKITYSIEYAAENNLP
                     IIICSASGGARMQEGMVSLMQMAKTSQALSKLEEKSLPYISVLTDPTTGGVTASFAML
                     GDIIISEPNTLIGFAGPRVIEQTINQKLPEGFQTSEFLLEKGFIDMIVDRRKMKEVLY
                     QILAMHKK"
     gene            complement(40738..42099)
                     /gene="accC"
                     /locus_tag="PC414_001208"
     CDS             complement(40738..42099)
                     /gene="accC"
                     /locus_tag="PC414_001208"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDF2463613.1"
                     /translation="MIKKILVANRGDIAVRIIRTCKELGIKTVAIYSEIDKDCFHRYI
                     ADESICIGPNNISKSYNNIENIIYLALKLKCDAIHPGFGFLSENPEFAKQCEDNNIIF
                     IGPTREQMILMGDKSRARETMMELNIPVVPGSESVLKTKEEALEVAREIGYPVMIKAS
                     SGGGGKGMRIVRKEEELFSNFDMASSEALAAFSNSDLYMEKFIENPRHIEVQVFGDKH
                     SNAIHLGDRDCSMQRRNQKVIEESLSPYLSDEERQKLHKIAVDIVKGVGYIGAGTIEF
                     IVDKDKNFYFIEMNTRIQVEHPVTEMVTNLDLIKLQISIANGDKIPFKQEDITFRGHA
                     IECRINAEDSSKNFAPSPGKIESLNLPGGFGVRFDTFVYAGYTIPPLYDSMIGKLICW
                     AETREECISRIYRALDEIIVEGINTNVEFQKALVTSEEFREDTHHTKFIEDVFMKKEF
                     ATL"
     gene            complement(42115..42567)
                     /gene="accB"
                     /locus_tag="PC414_001209"
     CDS             complement(42115..42567)
                     /gene="accB"
                     /locus_tag="PC414_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HDF2463614.1"
                     /translation="MNINEIKELLKAIDSTNLEYVKLESSDLRLEVSKKAQSTSSPVL
                     SVQQESVVDLSLEKPVVNDTPVTSNENLSVVVAPLMGTFYDSPSPDADSFVKVGDVVE
                     EGDTLCILEAMKLMNEITSEIKGEIIEVLVSNEELVEYNQPLFKIKPL"
     gene            complement(42837..43283)
                     /locus_tag="PC414_001210"
     CDS             complement(42837..43283)
                     /locus_tag="PC414_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463615.1"
                     /translation="MESKFFNWYTQSLGGTFGIISCICAYLNGYMFVYTNSASKYFES
                     IGFLGIISSYTLLPLCMLTLIFGVIYSYTPETSDKSIFNIPFFTLNKRLILITVVFGF
                     IGARVYFIIPAILITFNLYSNVLIAKLKYILCKNKENDTNLKESEV"
     gene            43558..43836
                     /locus_tag="PC414_001211"
     CDS             43558..43836
                     /locus_tag="PC414_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463616.1"
                     /translation="MVDTLEFGLKILFFILSIIWMGKIMILRTDKQIVINPLLIGISA
                     ILVMLHTSQSNIEFFGLDVQYIRIVLYIIYSLIILIGIWATNRRNGIF"
     gene            complement(43965..44711)
                     /locus_tag="PC414_001212"
     CDS             complement(43965..44711)
                     /locus_tag="PC414_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463617.1"
                     /translation="MWEGTMNLANLIPFIISAAALSPINSNDKVLSENQLKDIFPDAN
                     LRAVVKRYINPDEMTISNIKALDGEFYATGESISNLKGISYLENVDNFIFWNNNIKEV
                     PKEALSLKDMDSINLANNYLIDDDVVNSLSHNGVDVNCDLNFIDTKDNQYKLDSKYHN
                     VDIAKGESIDLRKIITKKIDSYYKYWEVTDNLPKDLNFIVSVSDKSVLSCEDMIIKGN
                     KTGKAVVKVILSDKNDLNTSQEVLITVNVK"
     gene            complement(44840..45484)
                     /gene="trmB"
                     /locus_tag="PC414_001213"
     CDS             complement(44840..45484)
                     /gene="trmB"
                     /locus_tag="PC414_001213"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HDF2463618.1"
                     /translation="MRRRRKKGADEKLLSYTKYVLRDDIDKLKGKWNLKFRNDNPIHV
                     EFGTGKGKFITTLAKQNPDINYIAMELKEEVLLKAVEKADASNLNNILFLWGDVSNIL
                     DYFEAKELSRIYINFCDPWPKNRWSKRRLTHSGFLEMYNRVLEDDGEIHFKTDNEKLF
                     EFSLNEIAANNWLLKNISLDLGNSEYENNVTTEYEDKFMSQGMRIFRCEAKKRN"
     gene            complement(45622..45942)
                     /locus_tag="PC414_001214"
     CDS             complement(45622..45942)
                     /locus_tag="PC414_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein HesB"
                     /protein_id="HDF2463619.1"
                     /translation="MKITLPETAIDTLKSILKDNQDKPNNIRVYFAGVGCGGPSFGLA
                     LDEKKEDDLTYEVGELQFVMSSDEYSQYGDIIIEDTGFGFRVIPENMKDQGGGGCSGC
                     SGCH"
BASE COUNT        17407 a         6141 c         7048 g        15513 t
ORIGIN      
        1 aaaatatata gaaaatcctt ataaaaattt aaattgtaat gaataggaag taattaaaaa
       61 gaatttttat tgatttttaa aaatttagtt agaaactata tcattatggt aaaaaatgta
      121 ttataatgtt tagaaatatt ataataagga gcgattaaac atgaatctat cgaagaaaat
      181 agaagataaa agaaaagcta aacagagagc tgaaaaagtt aagaaagcta aaattgctac
      241 agctggtgtg gtattaggag ctgttactgg tgcagtgagt ggagtacttt tggctcctaa
      301 atctggaaaa gaaacaagag aggatataaa agatgcttca caacaaattg ctgaaaagat
      361 aaatatgaaa actgtagatg taaaaggcaa agtatctgag aagctagagg ataaaaaagg
      421 aaactttata gaatcaaaga aaaaaataaa aaaatatata gataacaaaa agagtgttga
      481 aaattctagt gaggaagata aaataataga tatagttgaa ccagttagtg ttactgagga
      541 ataatatgga caatacaata acgattaaca taagtataat atatgtatta ttagctatac
      601 ttgcttgtat tgctttagtt tatttaatta ttgtacttgt taaactacat aagattttaa
      661 ataatgttgg aactatgtta aatgaaaata ctcaaaatat aaatacaact ttaaataatc
      721 ttcctaatgt tgtagaaact tttggagaag taggagaaaa tatgaaagat gtaacagaag
      781 tagttactga agttacagca gattttcttg ttactaaaga cagtgtcaaa tcaaatatgg
      841 atgttatagt tgatatattg actataatta aaaacatttt tctagataaa aaataataag
      901 tttaattaaa ataagcctat aagatataat ttaaacgacc ttaaaatagc atttataagg
      961 tcgtttttta ttagcttgat ttgacactat gtgtttttaa taattggtaa tattcctctt
     1021 tatcatttga acatatatat ctatgcttgt tgtttcaatt acatccataa gaacatcttt
     1081 acattcaagc tcaaaatctt ccattccttc taaatattca tcatcttcat caaaaaagat
     1141 ataagaatta tatagccatt cttcaaattt ttcttcaagt atatcccaat acgaactcca
     1201 aaataatcca caacctgata tataagatgc tctactttga tttatataaa cctcctctag
     1261 aagcttttgt aatctactgc tataaagaaa atctgctctt ccactgcttg atattaattc
     1321 atttaaatct tctattgaca ttaattcaac tttaccactt ttatcataag gagtataatt
     1381 ttcatcaacc catttatgtg gtttgttttc tctgcatact gttgctatca actctacaac
     1441 ttttgttctt atcatttttt tatcctaact tatttatttt ttataaaata atcatgattt
     1501 tataatttat ctatttcttg taatgctaat ttaacagttt catatctatc agcaccattt
     1561 attactacat caccttttac aagtttatta gcttctccac ctacaacata aaccttttta
     1621 cctaaacctt gcttataact agacgcttca attattaaag aatctttcaa ttgccaatgt
     1681 aaaatctgag cagccacttt atcagcatca cctgtataaa cgattacatt ttcatacact
     1741 ttattttccc ctccctcttg agaatttgat atagatttgt ttagtatacc ttctgctatc
     1801 aatttagcaa ctttatcttt attattaaca taataatctg tatctgcttt actatctaca
     1861 aaacatactt ctattaatat tgctggtgca atagtatgat taagccaata aagacctcta
     1921 acatcagact ttgcgcccct atctttgaat actgtagcta acctagtatt cactctttct
     1981 gcataggttt tgccattatt agtcttatat attgtttctg tccctactgg agttgaagtt
     2041 gtggcatttg aattaaaatg aatctgaaca gccaaatcag tttcctgttt atttgctata
     2101 gcacattggt ctgctaaatg attacttgat tcatcaactc ttcctgtata aacagtagct
     2161 ccacctattt taagccattt aacaattaag tcattcagta ttcttgtttc tttgctttcg
     2221 tttatatacc caacagctcc tgtacctttt ccaattaaag tgtgccctgg tattattact
     2281 actttcaaac taacatctcc tttacaatta aatttaactt ctatttaata tatgcataac
     2341 ctagaatttt gtttcttttt atatcttata cttttattat aacccatttt gtaaaaatat
     2401 agaatattaa gtaaataatg ttaaaattat aattttgtat ataagacatt acttgattgc
     2461 aataatttta tagcatattc atctattcta tatccagttc tataatatat atgtttaatt
     2521 ccagcttgaa ttatagcctt agtacaattt aagcaaggaa agtgagttac ataaatagag
     2581 caatttttaa ctgaaatacc ttcttttgca caataataaa gggcattttg ttcagcgtga
     2641 atggttctaa tacaatgtcc atctctcatt tcatgaccaa cttcactaca atgcttatct
     2701 ccagaaatac taccattata tccagtagac actattctgt tttctgaatt gacaattata
     2761 gctcctacat aagctctatc acaagttcct ctttcagcta ctgtttcgca cagtctcata
     2821 aaatattctt gccatgagca tctattttca agttttttat tcaaattata tacctccaaa
     2881 acttatatct aatatataag atttatacct taaaaataat attaaaaaca aatttatacg
     2941 ataaataaaa tttattaata ctgtttttag tctaaattat ttcattttaa attaaagtta
     3001 caaggaatct aaaattatat atatctttct tcgtaatatt aatgttacaa tataaaaaac
     3061 tgtctcaata aaatagagac agttttacag tcaaagttta taatacaagt tcatattttt
     3121 ctttaaattc ttttacagct tcgggccatg ccttttgttc tgtaatacct ttttctttag
     3181 ctattttaat tgcaatctct cttatcattg cagcgccttc taatattccc atttttaatc
     3241 tcccttcaaa tatctttttg tatagaatat aaatagtgct aagatttatt acatgctgca
     3301 tatacaatat ctatacaaca cataacttgt aaattccttt tattttcata cgtctttaac
     3361 cgacatataa ttatattcaa taagttttaa aattatgaac tcaattataa ttatttttat
     3421 ttttttagaa tctaaacact aaaaattgta ggtaaatttg tcttatgcct ttataaatat
     3481 ttctacagtt ttaattactg gacatatcta tttttattat aaaaagcgat taaaaatcga
     3541 gaatatccgc ataaaaactg tatataagca aaacggttaa aggaaaaaga agaaatttac
     3601 ttgaatgtta aaatgcgttg cttgaagcaa tgggcagttt tgcctataat atcggtaaca
     3661 actgaaagaa aggtggttag ccctaatgtt aagcgaaaac attaaaacaa tcaggaagtc
     3721 aaaaggactt tcacaacaag aacttgctat aaaactgaat gtagtgcgac aaacaatatc
     3781 taaatgggaa caaggactgt cagttcccga ttctgatatg ttgatttcca tatcagaggt
     3841 gcttgaaacg cccgtaagta ctttacttgg agaaactatt gttgagtcgg aagttgataa
     3901 cttaaaagtg atttctgaaa aattagagat tataaacttg caacttgcac aaagaaaaaa
     3961 catgagaaga aaaatgtttc attggttatt tattttatta tgtgcagtca tattaatagt
     4021 ttttgtggtc ttagggggac taaatagtcc ttacttggat tggaattata ccgaccctga
     4081 aaccgctgtt tttggagtag cttttcatgc ttttgagtgg ctatttgtca gattagtacc
     4141 gattgtcctt ataggagcaa ttattggaat ctttttgaca cgtaagaaaa tatgaagtaa
     4201 ctctgctttt tataatacag caagcgttcc ttaaaagcgt cattctacat ggcaatcgag
     4261 gacgactatg taaggaagaa cctttttgat ttccaactaa gcgaagttat tgaggacgac
     4321 agtaagacca aaaccgcatt aagtaaagta caggaagcaa gactgctgga cttatactct
     4381 catgcgtcag atgcaggaaa tgcgaggtct ggtagcatga tttacaacga tttgtacaac
     4441 gactgctagt agaaataata tggagataag tattatctac aaagtcagaa aagaaatgac
     4501 ttgataattt actcatgtta taattattga tgtgaaatta ataaatcgaa attccttttt
     4561 tggatttaat aatactgagt aaggaaaaag tgaattactt ttgcaaggag gtgttaatat
     4621 caggcaagta aagtcttttg tttttccagt tatatcctta atattcttag accaaattag
     4681 caaagttctt ataggattat tcttaatgga ctttgaaatt gatataattg ggaaattttt
     4741 aagattcaat cctgttcaaa atacaaatct ctcttatggg ggaaacttta ttggtattct
     4801 atctaattta tgggtattgg tattgtttaa cattttagtt atattagtta ttatatctgg
     4861 atatgctttt tataaatcaa aaaatgaaca aacaagctat tcagtaaaag taattatgtc
     4921 ttgtggactt gctggtacaa tatgtagctt gatagataaa ttattttggg gaggaagttt
     4981 agattttttg cagataccaa gcttttttat tttcgactta aaagactgct accttactgt
     5041 tgcagaaata atatttgttg tcataggaat tttgcataat agagaaatat caatgaaaga
     5101 atacatatat ttttgctatc gtcagtttaa aaaataaatt tagaagataa atttaagttt
     5161 caaggtaaac ttatttaatc acagcaaata gttggcttac ttaagctttt gggctaaact
     5221 gtgagcacta attaagtctc tgatatctaa caaacggagc acaaaaataa aaattaaata
     5281 taaagcacag ttaattttga ttaaaaagtt gactgtcttt tcttttgtag aaatttaagc
     5341 ataggtaata aagaaatgac tgcatttaag cattatgtat ataattggag gcaataaaaa
     5401 gccagcaaag cccataaaat agagctgtgc tagcaacatg atgaattata agaagataat
     5461 caaaaactta cgctttattt taatttaaaa aaatgattac tgtaaagaaa tcatttttta
     5521 ttataatcac tgatacatca aatattctaa ataaaatttt tatttatctg gaattgtata
     5581 ttttttctat aatattaact acagcgtaca ttgcccaagc acagatagcc aatacaaata
     5641 ctcccatcat tactaaatct aatttaaata cttgagaacc atatactatc aagtagccta
     5701 ttccatatct tgaaactaaa aattctccaa ctattacacc tacccatgcc ataccaatat
     5761 taattttagt taaatttatt aaatttcctg tgtttgcagg taatatgagt ttaaataata
     5821 tttgagattt agtagctcca aaacttttta acatcttaat tttttcttca tctatattca
     5881 taaaataatt ataagctgaa agtatagtaa caacaacaga aatcgttaca gctgttacga
     5941 cgattccttc tattccagca cctacccaaa ctataattat aggagctaaa gcagtcttag
     6001 gaagagcatt taaaactacc aaaaatgggt ctaatatttt tgataatttt tctgaccacc
     6061 aaagagcaat tgctaccaaa atacctaata cagtacctat aacaagacca agaacagttt
     6121 catatacaga tattccaata tgtttaaata attgaccact aaatgcatat tgaataaata
     6181 aattgtaaat atcacttggt ttgctaaata gaaatgtatt tataacattt aagtcagcta
     6241 aaagttccca gattacaata aaaccaatta aaattaatag ctggtagaat aatacatttc
     6301 ttttttctct ttttacacct ttaacatagt ttatatatcc ttcagatctt ttattaagcc
     6361 tcatgtgtat ctagctcctt ccataatatg tcgaaatact tttgaaaatc aggaacttta
     6421 cgtgttttga gaggcgtttt tgttccatcc atgttaagtt caatatcgta aatatttttt
     6481 atggtagctg gtcttttaga tagtattgca accttatctg atatgcttat tgcttctgct
     6541 atagctaaaa aagtgcaact aattgcttaa agaaacagaa ttatcaactt cagcacccat
     6601 ataaacaata ttaagatttc ctaaactgct atcccaaacg atactattaa taatagtaga
     6661 taataacaat ctcttatcat ctaaactggc attatcaact tcatcattaa atctcttcaa
     6721 attactaaca ataatatcaa tattaatact gtcactacta atattgatgt tactagattc
     6781 caaagtatta atcttatcct ttaaaatact aatctcatta tttaggctct caatctggga
     6841 aactatataa tcagaagcag tactaccagt attttttgca agctctataa ctaaattttt
     6901 aataagtctt tccttatcta aaatttgatt tttaagtaaa ttagtctcag atttaaaaat
     6961 attaaaaaca ttatcagtct taatgttatt actattgaat gcacttataa tagactcttc
     7021 tttacaattc ttaattttat ctataactaa actttcaact aatggaccat taagattttt
     7081 acagttgcaa ctatcagacc caagtgtttt tttagtacca caaatataat aatactttaa
     7141 atccttgtca gtttttttgc ctttataggt aattctcata ttagaaccac atttaccaca
     7201 ttttataaga ccagataaaa gagcagtttt tccagttgat atccttggag ttttatcacg
     7261 atttttatct aattgatttt gtacttcaag ccatatttca ggttctatga ctccatggtg
     7321 ttttgcaatt gctgctattg agttagatgt atttttagca taggtaagga ttccacattt
     7381 tctatctgga gtacctacaa catccatacc actattttta aggtaatcta aaatttcatc
     7441 attagctttt acatatgctg gatttctaag tattctagat aaagaactag ggtcaaattt
     7501 gccaccacga ggacccctta ttccattatt atagacatat ttatataact tagataagga
     7561 cctaagttct aaatatttat tgtatattag ctttacaatc tccatttgtt catcatttac
     7621 ttgaagctta tacatacttc tttcttttag attttcatct aagaatgtga ttttttcact
     7681 gttgaatcca tgaggtgtat tgcctccaag ccatctacca ctacgggcta attcatacat
     7741 attgtctttt atacgttctg ctattgtttc tctttctaat tgagcaaata cacttgatat
     7801 aaacatcata gctcttccca ttggagttga agtatcaaat tgttctttga tagatataaa
     7861 tgatatgctt agttcattta atttttctat taaagtagag aagtcagata catttctcga
     7921 tatcctatct aatctataac aaactaggta actaaatttc ttatctttgg catcctgcat
     7981 cattttctta aattgtggtc tatctatatt gctaccacta aatccctcat cttcatatat
     8041 caaaaaatct tttacaccaa tagactttgc ataatcaata cataattgta tttgattttc
     8101 tatagaatca ccttttccag taaatcttga ttttcttgaa taaattgcag ctttcaaaat
     8161 taaaaccccc tataagttag gatatatatt tgaaactatt tatctacaac cttttaagta
     8221 aacttattag aatgaattta ttaattaggt aaatttatat aattctactt tttataatat
     8281 atgactattt attttatttc tttactattc aagttctaaa tcaataataa attttgatat
     8341 ttaattgtat caggacaaat ctctaatttc atagattatt aatataaaat cataggttag
     8401 tgtgaaaaag ttgaaattta aatattttaa aagataattt ttttgaacta ataggtataa
     8461 caaaaattta tagggggatt gcatatggaa atcaatgtta tagaaatttt ccctaaagat
     8521 aaagctaaac ttaataaaat agaaatggat aaagctagtt ggtttgtaaa tataataagt
     8581 aaaaaatatc ctaaagaagc tttaaatgaa gcatttagta ctttagaaaa agaattaaat
     8641 ataagtaaag ctaatacata agctttgatt attgatttta tatactattg gttttatacg
     8701 ctatgacttg acattatatt aatcaaatag tatattttta aatatagcac aatgaagtgt
     8761 taaaagagca atggagctcc atagataaag tctatggagc ttttttgcgt atttaactta
     8821 aggaggatag gactttgaat aactttgaga ttaaaactag aataaaattt ggagaaggct
     8881 cactaaaagc tttgcaagaa ttaaaaaaca aaaatgtact aataataaca gaccctttta
     8941 tggtgaaatc aaaaacaata gataaaataa catctaattt aataaactca acatatcaag
     9001 tttttgaaga aatagtacca gacccaccaa tagaagtagt agtagcagga atagaagcat
     9061 ttaaatctat aaatccagac gtaataatag cattaggtgg tggttctgca atagatgcag
     9121 caaaatctat aatggacttt tctaaaaaaa tattaaaaat taacgaagta gagtttatag
     9181 caataccaac aacaagtgga acaggttcag aagtaacatc atttgctgta ataacagata
     9241 gtcaaaaaaa atgtaagtat ccattagtga gtgatgattt acttccagat atggcaatac
     9301 tagacccaga acttgtaaaa actgtaccaa attttataac agcagataca gggatggatg
     9361 tattgacaca tgctatagaa gcttacgtat ctacaaattc taatgacttt tcagatgcac
     9421 tagctgaaaa agctataaag ttagtattta aatacttaat aaaagcatat aaagatggaa
     9481 atgacataga agctagagaa aaaatgcaca atgcatcttg tttagcaggt atggcattta
     9541 accaagcatc acttggcata aatcattcta ttgcacatgt attaggtgga aaattccata
     9601 taccacatgg aagaacaaac tctatattac ttcctcatgt aatagaatat aattcatcaa
     9661 tagtaggata tactaacaca acttactctg ctacagctaa aaaatatgca caaatagcag
     9721 aattagtagg acttgggagt gctaatataa gaatatctgt tagaaattta gtaaatgaaa
     9781 taaagaaact acaaaaagaa atgaatatgc caactaaatt gacacaatgc aaattaagtt
     9841 tagaagatat aactaatgaa gaaagagaaa tagcaaaact tgctataaaa gatacttgta
     9901 cattaactaa tcctaaggta ccaactgaag atgatataat aaacatatta aagaacatta
     9961 aataactaca ttttattaca tgtaaatata atattataca aatagggaaa atgtttttaa
    10021 aaatataaat tatatattga caaatataaa acataaaact ataatgaaag tatcacaaag
    10081 gcgtgatact ttagtagaaa gcaatggagc ttcttaggaa tattaatatt attcttatgg
    10141 agctcctttt attttaatca aatagctttt taataaacta tattcaaaga aagcagggaa
    10201 taaatttgac tgaagaatct aagcaaagag ttattcaaga atatgttcct ggaaaacagg
    10261 ttacattagc acatatcata gctaacccta atgaagatat atataagaag ttaggattag
    10321 tacttgataa aaaagatgca ataggaatac ttacaataac accaagtgaa gcgtcaataa
    10381 tagcagcaga tgttgctaca aaggcttcaa atgtatcttt aggttttata gatagattta
    10441 gtggttcagt agtaatatca ggtgatgtaa gttctgtaga atcagcgtta aatgatgttt
    10501 tagaagtctt aggaaatatg ttaaacttct catctacaaa aatcacaagg acataataat
    10561 acaatgaaga atataatatt tatgggaaag acaggtagtg gtaaaactac cttatgtcaa
    10621 aaactagacc agttagaatt aaagtataaa aaaactcaat cagtggaact ttataataat
    10681 gcaatagaca ctcctggtga gtatatggaa aatagaaacc tatataatgc cttaataaca
    10741 acatcagcag atgctaaaat tatagcaata gtgtatgatg ccacacaaga agaaaattat
    10801 atagcaccag gttttgccag tatgttttgc aaagacgtaa taggaattat aactaaaata
    10861 aataaagcta ctgaaaacga agtcaatata gcttatgaaa gactatcact agcaggagca
    10921 agtcaaatct ttaaaataga cacagttgat aatattggac ttgaaaaatt atttaaatat
    10981 ctagaaaaat ataaaaatat agatgaagtt taaggggaaa tattttttta ttcaaaaata
    11041 ttattaaagt atgataaaat aaaaaaatgt ataaactatt ttaagaagat ttaatcttta
    11101 agcgaggtag gaacatgggg aaaaaaatac tattggttga tgatgaacca ctgttaagga
    11161 tggatacaaa agatatatta gaaagtcatg gctatgaaat aataggcgaa gcaagtgatg
    11221 gatttgaagc tgttgaaatg tgtaaaaaac acaaacctga tttagttata atggatatag
    11281 agatgccaat acttgatggt ataaaggcag gcaaaataat atgtaaagaa aatatagctg
    11341 gtggagtact tttattaact tcatttgaag ataaagaata cgtagaaaag gctaaaagta
    11401 taggtgctta tggatattta gtaaaaacag caagtgaaaa atcacttatc ccaaccatag
    11461 aaatgtgttt aaataaggta aatgaatttg aaaaaatgaa aaaagattta gaaaaagtaa
    11521 attctaaatt gaatgaaaga aaattagtag aaagagcaaa aggtatactt ataagagagt
    11581 ttaagatttc tgaggatgaa gcatacacta gaataagaaa attaagtatg gataaaagaa
    11641 ctacaatgat ggaaattgcc aaaatgatta taataggata cgatgactaa tacgataaaa
    11701 aaaatatcaa ctgcacaaac atacttaaat aataacgaaa ttgaaaaaat tatagaagta
    11761 tcaaaatcct tagatttaat ggcttctttt tatgaagctg atgtttttat agatgtacta
    11821 agtaaatttg atgatgaagc aattgttgtg gcacatggta gacctaaaga aaagtcaatg
    11881 tactgtgaaa atgttatagg taaaaaggct ttaattgaaa atgaaccagg agttataaag
    11941 tgcttaaaaa caggagaaag agtaagagat attaaagctt taacacagga atataaactg
    12001 gtcaaacaaa atattcagcc aataacacta gatgacaaag ttttagcagt actaatagtt
    12061 gaaaaagata taagtagtga agttaagaat gagtttaatg ctagtgaaga cgaagaaaat
    12121 aaaaatttac atcagtttat aaaacttatt ggaaataatg actttttagt tgataattta
    12181 aattcagcca tattaatatt tgataaaaat ggaaaattaa aaattaaaaa tcgtattgca
    12241 actgagatat acaaaaatct tgggtttgga tatgatatac aagatatgca ttacaatgat
    12301 ttagatatag aaaccaacac ctttgaaaat atattaatgg atgaaaatta tgatgagaca
    12361 aatgaagttt caataggtaa tatgtttttt aaaataaaga ccatatacat caaagatgat
    12421 gaatttaagg tttgcaaaat agtacaagat ataagtgatt taaagagaaa agaagaagaa
    12481 ttagttttga aaacagtggc tataagagaa gctcatcata gagtaaaaaa taatcttcat
    12541 acagtaatat ctataataaa aaaacaaagt agattatctc aaaatgatga agtaaagcag
    12601 tgtttagata acataataaa tagggtattt gcaatacttt cgactcatta tttactatct
    12661 aaagaagttg ataataatat atctatacga gaaggtatca atttattagt gtcaaatatt
    12721 caaggtggct atttatttag taaggatata gatatacgta taactggtga agattttaaa
    12781 attagtggag aaaaagcaac agcaatactt ttggttataa atgaaatact acaaaattgt
    12841 tatgaccatg cattttcgga tagagaacat ggtactatac aaatattaat aaataaagat
    12901 aacgactata gaaatattgc tattatagat gatggaattg gatttgatag taataatata
    12961 aataaaaata gcttagggac atatataata gatagttata taacacagat gttaaaagga
    13021 aatatagaga gaaaaagtag tgagaatggt acagaagtat taattactat acctacacaa
    13081 attaatattt aaataataac tcatctacaa agtagtagtt ttaattgagc aaagaggctt
    13141 aaggttatta atagattaag ttaattgaat gtatttataa aaattataag tatatattca
    13201 atttaacagg tatttattat aactttaagc ctctttgctt gtttaagaca tacttaaatt
    13261 ttaggaggtg agaaaatgag agaagagtta ttaagtgttg gtattgacat tggaacaagt
    13321 actactcagt taattttttc taaattaata gtagaaaata ctgaatcaaa tttttcggta
    13381 cctagaataa gtatagtaga caaagaaatt atttataaaa gcaaagttta tttcacccct
    13441 cttatttcaa ataatgaaat aaatggtcaa caaataaaag atatagttga aaatgaatat
    13501 aaaaaagcaa atatatctaa agaagatata catacaggag cagtaataat aactggtgaa
    13561 acagctagaa aagaaaatgc aaatgatgta cttcatactt tgagtggatt tgctggggac
    13621 ttcgttgttg caacagcagg acctgattta gaaagtataa tatcaggaaa aggagctgga
    13681 gctcatattt attcaaaaga acattcaact acaattgtaa atatagacat aggtggggga
    13741 actagtaaca tagcctcatt taaaagggga gaagtaaccg atactgggtg tttggatata
    13801 ggtggaaggc ttgtaaagat agataaaata agtaaaaaaa taacttatat atcacctaaa
    13861 atagataaaa tcataaaaga taataattta aatcttcaag taggcacaat ggctactgta
    13921 gaaaatacaa agccaatagt ggatataatg gttgacttat taatggaaag cataggatta
    13981 aagaaacaga cacctttgtt tgattttata ataaccaata aatcaataag tatagatgaa
    14041 aatattaaaa atgtttcatt ctctggtgga gtagctgatt atatttacta tgatggagaa
    14101 ataaaagatt attttgaata tggtgacttg ggaattttac ttgggcaagc aataaaaaat
    14161 tgtgaatacc ttaaaaaagt aaatgtagtt aaatctatgg aaacaataag agccacagtg
    14221 gttggagcag gttctcatac tactgaagta agtggaagta caataactta cactagcgaa
    14281 aagtttccac ttaagaactt gccagtttta aaattatcaa ttgatgaaga aacaggcact
    14341 tcaaaacaaa tcagtgaagc tataaaaaat aaacttaatt ggtttaaact agaaaatgat
    14401 ttacagaaag ttgctatagc catgatagga aagaaaaatc caaccttttt agatatacaa
    14461 gagtatgcta aagggtttgt agatggaatg caacaaatca tagaaaatga tttacctttt
    14521 atagtaattg ttgaaaatga tatggcaaaa gtgttaggtc aagcaatata tgcattatta
    14581 aattatgaaa aagaaattgt ttgtatagac agtgtcaagg ttgaaaatgg agactatatc
    14641 gacattggaa aacctatagt tgaaggtaaa gttttacctg tggtaataaa aacattagtt
    14701 tttaattaat agaaggaggt aagtaaatga ttttaaatac aaaattattt ggacgtactt
    14761 atcagtttaa aggcttatat gatgtaatgg ctaaagctaa tgaagaaaaa tcaggagata
    14821 aattagcagg tcttgcagca gaatcagctg aagaaagaat agcagcaaaa gttgttttat
    14881 cacacataaa attggaggat ataagaaata atcctgcagt ggcatatgaa gaagatgaag
    14941 taacaagaat aatacaagat gctgtaaatg aaaaagttta tgaagaaata aaaaattgga
    15001 cagtctctga acttagagaa tggctattgg attcaaatac tacaagtctt gatataagaa
    15061 gaatatcaag aggattaact tctgaaatga tagcagcagt tgcaaaatta atgtcaaata
    15121 tggacttagt ttatgcagct aaaaagataa aggttactgc tcactgtaat acaactatag
    15181 gagaacaagg aacattatca gcaagacttc aacctaatca tccaacagac gacccagatg
    15241 gaatacttgc atcacttctt gaaggtttaa cttttggtat aggagatgca gtacttggat
    15301 taaatccagt tgatgactct gttgagagtg ttactaaagt attaaaaaga tttgaagaag
    15361 taaaacaaaa atatcaaata ccaactcaaa catgtgtatt ggcacacgtt actacacaaa
    15421 tggaagcaat aagaaatggt gctcctgcag atttaatatt ccaatctata gcaggttctg
    15481 aaaaaggaaa tgaagcattt ggatttaatg gcgacactat agaagaagct agacagttag
    15541 ccttaaaaca cggtacagct acaggaccta acgttatgta ttttgaaact ggacaaggtt
    15601 cagaattatc ttcagaagca catcatggaa tagaccaagt tactatggaa gctagatgct
    15661 atggatttgc taaaaagtat gaaccattct tagtaaatac agttgttggg tttataggac
    15721 ctgagtactt atatgatgct agagaagttg taagagcagg acttgaagac cactttatgg
    15781 gtaaactaca tggcttacca atgggtgttg atgtttgtta cactaaccat atgaaggctg
    15841 accaaaatga tatggaaaac ttagccatac tattaacaac agcaggatgt acgtatttta
    15901 tgggaatacc tcatggagat gatgttatgc ttaactatca aacaacagga taccatgaaa
    15961 cagcatcact tagagaaatg tttggactaa cagcaataaa agaatttgat gcttggatgg
    16021 aaaagatggg attctctaaa gatggtaagt taacagagtt ggcgggagac gcatctgtat
    16081 tattaggata ggaggtattt aatatgaatg agaaggattt aaaagcattg gtagaacaat
    16141 tagttggaca aatggttgga gaattagata ctaatgttgt gtctgagact gttaaaaagg
    16201 caactgaagt tgtagtagac aacaatgctt gtatagatga tataacagaa gttgatataa
    16261 gaaagcaatt actagtaaaa aatcctaagg atgcagaagc ttatttagat atgaaagcaa
    16321 aaacacctgc aagattaggt ataggaagag ctggtactag atataaaaca gaaacagttt
    16381 taagatttag agcagaccat gctgctgcac aagatgctgt attctcttat gtagatgaag
    16441 aatttataaa agaaaataat atgtttgcag tggaaacttt atgtaaagat aaagatgaat
    16501 acttaacaag accagatttg ggaagaaaat tttctccaga aactataaat aatataaaat
    16561 ctaaatttgg tacaaatcaa aaggttttaa tattagttgg agatgggctt agctcagctg
    16621 caatagaggc aaatttaaaa gactgtgtgc cagctataaa acaggggcta aaaatgtatg
    16681 gtatagattc aagtgaaatt ttatttgtta aacattgtag agttggtgct atggaccact
    16741 taggtgaaga attaggttgt gaagttatat gtatgttggt tggagaaaga ccaggattag
    16801 ttactgctga atcaatgtca gcatatatag cttataagcc atatatagga atggcagaag
    16861 caaaaagaac agttatatca aatattcaca aaggtggaac aactgcagtt gaagcaggtg
    16921 ctcatatagc tgaacttata aaaactatgt tagataaaaa agcttcagga atagacctta
    16981 aataaagtcg agggagtgta gaatatgaaa aatgatgtaa ttcgtcctaa tatactagga
    17041 attaaaataa tttcaaatat aagtccagaa atggcacaaa aactagaatt aaaatcacat
    17101 cacaaaagct taggtttaat aactgcagac tgtgatgatg ttacgtatac agctcttgac
    17161 gaagcaacta aagcttcaga agttgatgtt gtatatgcaa aatcaatgta tgcaggtgct
    17221 gcaaatgctt caacaaaact tgctggtgaa gtattaggaa taatagcagg tccaagccca
    17281 gcagaagtaa gaagtggtct aaatgcagta gttgattttc tagagtatgg agcaactttt
    17341 ataagtgcaa atgatgatga ttcaatagca tactatgctc actgcgtatc aagaacaggt
    17401 acataccttt cagaggtagc aggaataaga gaaggtgaag cattagctta tttagtagca
    17461 ccaccacttg aagcaatgta tgcactagat gcagcaatga aagctgcaga tgttaagatg
    17521 tgtgaattat ttgcacctcc aactgaaaca aactttggtg gagcattact tacaggttct
    17581 caatcggctt gtaaagcagc atgtgatgca tttgcagaag cagtaaaatc agttgcagac
    17641 aatccaacag gattttaagg atagttagat ggatgctata ggcttaatag aagtaatagg
    17701 atatgttgct gcaatagaag cttctgatgc ttgtttaaaa tctgcaaatg ttaatattgt
    17761 aagagttgat aaggttggag caggtattgt tactttaaca atatgtggag atgtcggtgc
    17821 tgtaaaagct gctttagaag caggtgaaat agcagcttcc agagttggta cattaagaac
    17881 atcacatatt ataccgagaa tccataatga agtaacaaat gctttattca aaactaaaga
    17941 acctaaagtc tgtgaagttt cagaagataa agaaaaaaac tttgagatta cattggaaat
    18001 agctgataat gtatcgaaaa atttatcaga aaaagaaaat gtaattttag atgtcaatga
    18061 aaacagtgga gatactgaaa atttatatag tgatatagat aaaacaaaca aaaaaattga
    18121 aacagttgat gatagtttaa cagaagctaa tgaggaaaaa atagaaaaca ctaatgataa
    18181 agaagtattg gaacaagtta aaatagaaaa ttccgatttt gatagagatg acctatcaaa
    18241 gaacagtgta aaaaaactta aagctatggc taagaaatta gattcttcaa taacttataa
    18301 agacttaaat gttcttaaaa aagaagaatt aataaactta gttaaaaagc ttagtagaag
    18361 ggatagatag ttatgacact tttagataaa gatttagtat ctatacaaga agtaagaact
    18421 cttgtaagaa gatctaaagt ggctcaagag aaactttcta aaatgagtca agagcaaata
    18481 gataaaatag taaaagctgt tgtagatgca gcatttaata atgctactaa attagctaaa
    18541 atggcaaatg aagaaactgg atttggaaaa tgggaagata aagtaattaa aaatactttt
    18601 gcatctatgg gagtttatga aactttaaag aacacaaaaa ctattggaat aattggtgaa
    18661 gataaagaag ccaatacgat agatatagct acaccagttg gagttgttgc aggtcttata
    18721 ccttcaacaa acccaacatc aacagtaata tacaaaacta taatatctct aaaagcagga
    18781 aatactatag ttttctctcc acatccaaat gcaaaaaact gcatacttga aacagttaat
    18841 ataataagtg atgctgctat aaaggctgga gctccagagg attcaatatt atgtataact
    18901 aatccaagta tagttggaac caatgaattg atgaaacata aagatacaaa cttaatacta
    18961 gcaactggag gagaagcaat ggttagagct gcttactctt caggaacacc agctattgga
    19021 gttggaccag gaaatggacc agcatttata gataaatcag caaatgttaa attagcaatc
    19081 aaaagaataa tagattctaa aacttttgat aatggtgtta tatgtgcatc agaacaatct
    19141 atagtaatag aaaaatctat ggaagaaact gttttaaaag agcttaaaaa tcaaggtgca
    19201 tatgttttaa atgaggagga aagtgaaaga ttagcaaaat tcatattaag agctaatggt
    19261 actatgaatc cacaaatagt tggtaaaagt gttgaggtaa tagcaaattt agcagactta
    19321 acaaatgtac ctaaaaatac aaaagttcta gttgcaagag aaaataaagt tggtcacaat
    19381 gtagcctatt caagagaaaa acttacacca atactagcgt tctatgttga agaaaatgta
    19441 gaagctatta tgaacagatg cagagaaata ttgttaaatg aaggtgcagg tcatacattc
    19501 tcaatgcatg taaatgatga agaattggta aaaagatttg cccttaatat gccagtatca
    19561 agaatacttg tgaatactcc ttcagcactt ggaggaatag gtgcatcaac aaaccttgta
    19621 ccagcactaa cacttggttg tggtgcaata ggtggaagtt caacttcaaa caatatagga
    19681 ccacttgatt taataaatgt taaaagagta gcttatggaa tcaaagaaat agaagaactt
    19741 ggaagagata aatctcaagt tgaaaaatca atgtttgact caatggataa agaagaatta
    19801 ataaatcttc ttgttcaaaa actagtcaaa gaactagcat aactaaaata aacaatgaaa
    19861 cttatggcta tcgctataaa tttctaaaaa tataaactat aaaaaactat aaaattaaat
    19921 ttttggagga taagattatg gcaagtgcaa acgcattagg aatgatagaa actaaaggat
    19981 tagtaggagc aatagaagca gcagatgcaa tggttaaagc tgcaaatgta caattagtag
    20041 gaaaagagca agtaggtgga ggattagtta ctgtaatggt aagaggagat gttggagctg
    20101 ttaaagctgc tacagatgca ggagctgccg ctgccgagag agttggagaa ctaatatcag
    20161 ttcatgttat accaagacca cactttgaag tagatgctat attaccaaag gtatcagctg
    20221 agtaatataa gaataattat aaaatttaaa ttttgattga aaaagaggct atctcaaaaa
    20281 tgagtcttac taataaactc aatgtatttt gaggtagtct tttttataca tctaatagga
    20341 gatgtgataa aatgggtgtt ttaacagaaa acgaagtaaa aagaaaaatt ggaaaaaaca
    20401 aaaataatat aactgaattt tatatagaaa aaggtcaaat aataactcct tctgcaaaat
    20461 cttatttagc tgagagaagt attgagctta aatatgttga taaactttca gatgtaaaca
    20521 atgaacctaa agaagttgta aaagaagtag ttaaagagaa aaaatataga tatactactg
    20581 tatttggcgc taaattggat gaaaaaccag aacatatgac tcaattaaat ggaaatcttt
    20641 tagtttttaa agaccataaa agaataatat tgagaggaaa aatagattct cttgaatcag
    20701 aaatactaaa ggtacagata ctttgtcaga aactagatat gccaaagctt gttaaggaat
    20761 tgcaagaagt attagacttt gtaagaaata taatgagatg tgaagtttta gatgaaaagc
    20821 taggggactt taaactttta ggattaactc ctcaagaact tagagaacaa tctcactatc
    20881 ctaaaaaata ctttggtata ggacatgagt tcccaagtta tgaaatggga gaagtagtag
    20941 tagctataaa tagtataaga agtaatacac gagaggttga acttgcagcc tatgaagcat
    21001 ttaagggtga gtatggaaat gttcacagag aagatttaat aaaggcactt aatagattat
    21061 cgtcagtatt ttggataatg atatataagg ttagaactaa taagtataag tagttggagg
    21121 acataatgga gaatctatta aatgaaatag tggaagaagt aataactaga gttaaaaaag
    21181 aagcatttat agaagttgaa gcatcaggaa gacacgttca cttaagtaga gaagatgtcg
    21241 ataaattatt tggagaagga tatactctta caaaactaaa agacctatct caaccaggtc
    21301 aatatgcatg taaggaaaga gttacaataa ctggtcctaa aggaagtata aaaaatgtag
    21361 ttgtacttgg accatgtaga aatgaaactc aggtagaaat atctttaaca gatggttcaa
    21421 ctcttggact aaaagctcct ataaaacaaa gtggagactt agaaggtact ttaagtatta
    21481 aaatatcaac acaatatggt gaagttgaac ttgataaggg gttaatggtt gctaaaagac
    21541 atatacacat gactcctaaa gatgctgaaa aattcaatgt atgtgataaa gagatagttg
    21601 aagctaaggt gatgggtcaa agacctttaa catttgatga tgtagtaata agagtgagtg
    21661 acagttttaa aacttatatg catatagact atgatgaagc caatgcatgt ggttattcaa
    21721 aaggaactgt tgctaaaata ataaagaaag cgtaaattta acctactata taaattacct
    21781 ctagggagga tttaatatga attatgataa ccttgtagat ttaataacgg aagaaatcta
    21841 taaaaaaata aatagtaatg aaataaaaat atctaataaa cctaaagctg tgattgtatt
    21901 tgaacaagat aacaataaat ttaacttatt aaaagatgaa tttgaaatag tagaatttga
    21961 taaaagtata agggaatgtg aaatagtgat agtttcaaga ctttgtatga gggggctatc
    22021 gaatatagca cttggaaatt caacaagtga tgaagaaaga tttatactaa aaatgattat
    22081 gaagggcaaa gaagtatatg tcctagatga aggaattgaa tacaaaaaat ataaggatac
    22141 tgcaccaaaa gcattatata agaaatttat gtcatttgaa gatgaaatat gtaaatttgg
    22201 tgtagaaatt ataaaagact taaatgttat aactaaaaat aagttaaata taaagaaaga
    22261 actagaaagt actagaggtt ctaaaaaaga agatacaaaa gttttagata aagatgtttt
    22321 aaatttaacc agtgtagaaa gtgtaaaaat agataatgaa tttagtttag atttaaggaa
    22381 taaaaaactt atatcggaag ctgacttaag aaaaccaact ataaatggag ttaaaaacat
    22441 attagttaat aaaaaaagta taataacacc attagctgta gattttatga gaatacacca
    22501 tctcaaacta aagaaagtgt aaggaggtaa ttttatggat ataggaaaag ttgtaggtaa
    22561 tgtttgggct acaagaaaag atgaaaaatt atgtggccaa aaacttttag ttgtaaaaat
    22621 tttaaaaact aaagacacat acaaggaagg cttcatggtt gcagccgata atgcaggagc
    22681 aggaaatgga gacttagttc ttatatccaa aggttcctca gctagagagt cgattgaaaa
    22741 atctcatgtt ccggtagatg caactatagt tggagtagtc gattcgtttg aggtgtttga
    22801 tgaataaaag tataggagct atagagttta aaagcattgc taaaggtata gaagtatcca
    22861 atgaaatgat aaagaaatct tcagttgatg tattgtactt aaaaagtatt tgcccaggta
    22921 aatttctaat aatagtaggt ggagaaactt cttatataaa tgaatgtgta gattatggaa
    22981 taaaacttgg agaaggatat attgtagata acttcgtaat aaatgcaatt tcacaagaaa
    23041 tattagatgg atttaaaaat aagtatcaaa agctagatag tatagtctct ataggcgttg
    23101 ttgaaagtag taaagtatgc gctggaataa aaatgcttga taagacttta aaatctggcg
    23161 acttagtatt agtaaaactt caattatctt ttgctatagg tggcaaatta gtatatatag
    23221 tagcaggaga tttaagtagt ttagaatatg cattaaaaga aagtgaaaat gtagtaagag
    23281 aaaaagaagt aatatataag actgtaatac catcagtaga cagtcaaata ataaaaagtc
    23341 taataaaata atggggggaa ttatgagtat aaacgaaatt ataatctatg taatggtagt
    23401 cttcatggta cttggagcta tagataaatg tataggtaac aaatttggtc ttggagaaca
    23461 atttgaagaa ggtataatgg caatggggtc acttgcagtt gcaatggttg gtgttatatg
    23521 tttagcacca gttcttgctg atgtattaag accaattgta gtaccagtat ttaatatact
    23581 tggagctgac ccagctatgt ttgctggaag tttattagct aatgatatgg gtggtgcacc
    23641 acttgcactt gaacttgcga aagaccctaa tgctgggtta tttggtggat taatagtagg
    23701 ggctatgatg ggacctacaa tagtatttat aataccagtt gcgcttggaa taattgaaaa
    23761 agaagaccaa aaattcttag caacaggaat acttgcaggt ataataacaa tacctattgg
    23821 agcattcgtt ggtggattag ttgcaggatt cgaggtaatg atggttctta gaaacttagt
    23881 gccaataata atatttgcag tagttatagc acttggatta ttaaaattcg aaaatgctat
    23941 gataaaagga ttcacatact ttggaaaagg tgtagtaata gttataactt taggacttgc
    24001 tgctgccata gttgaagctt taacaggaat agttgtaata cctggaatga aaccaatcag
    24061 tgaaggtata gaaatagttg gagatatagc aatagtttta gcaggagcat tcccattagt
    24121 gtttgttata acaaaggtat ttaataaacc attaatggct ttaggaaaag ttcttggaat
    24181 gaatgatgta tcagcagcag gccttgttgc aagtttagct aactgtatac caatgtttgg
    24241 tatgatgaaa gatatggaca atagaggaaa aataataaat gtagcattct ctgtatcagc
    24301 agcattcgta tttggagacc acttaggatt tacggcagga tttaattcta atatgataac
    24361 accaatgata gttgctaaat tagttggtgg tataacagca gtattgttag ctatggttat
    24421 cgcaaataag acactaaaga aggagaatgt ataagatgga tatatcaaat atagataaaa
    24481 atttaattga aacattagta agacaaataa tagaagaaaa aataagtgga actaaagaca
    24541 cagtagattt tgttagaaat aaagacatta gtggaataac ttctataaag ttaccaactg
    24601 taaaagttag tgaatctgat agattagata cagggaatcc aagtgatgta gtttacacaa
    24661 aagatttatt tacattagag gaaagcccaa gattaggttg tggtatgatg gaaatgaaag
    24721 aaacaacttt tgattggact ctaaactatg atgaaataga ttatgtaata gatggaactt
    24781 tagatataat aatagatgga agaaaggttt ctgcttcttc aggtgaatta atatttatac
    24841 caaaaggaag taagatacaa ttttctgtac ctgattatgc tagatttata tatgtaacat
    24901 atccggcaga ttgggcatct caaaattaat aaaaatcaag ttaagttaat aataatcaaa
    24961 taaataattt aaaatacaaa ctcttaaata agcacacata tttaaaaaag ctatgaatta
    25021 aaattcatag ctttttttat aaagaatgtt atattttaga cgtatttttt ctatatttac
    25081 tgggagacat attatatttt ttattaaata atctaataaa atgctctaca tttggataac
    25141 caatactatt ttactgataa tcttttcctt atcactaatc ttacatttta aaatattatt
    25201 tttactcgta atattatcat aagaatttgt ttcttattta aaactaatta gaattataaa
    25261 gtttattgcc atgttattaa taaaataaat ccagttaaat cgagtgctag ttaaagtaat
    25321 aagaaacttt tacaatggtt tcctttatcg attgaaataa aagttaatat ggtataatat
    25381 ctaaagagag aatcttatca attagaatat gcatagaaac aagtggctta aaaggagacg
    25441 ttatatggaa gagatagtaa agaaggaaca agcaagtatg aatgatattt ttttgtatct
    25501 taaagctaaa tcaaactcat tatataaatt tgtaatatta tataatgagt ttatcaataa
    25561 acctaaagat tatggaactg gggaacttat aaacatggtt gaagtacata tgctaacagc
    25621 gattgaagaa aatccaggaa taagtccaac tcagttagct aaaatatgga acagaactaa
    25681 aggtgcaata tctcaaacta tatctaaatt ggagaaaaag ggttatataa ctaaagaaaa
    25741 gaaagaagga aatgcaaaaa ctatattact atatccaacc acatcaggag tagagttaag
    25801 taaggcacat aaactatatg acaccataga tgttacaaga acatttgaat acttaatcaa
    25861 ggagtgtaca ttagaggaga tagatagttt ttataaagta attgatttgt ttattaattt
    25921 attagaagaa tctccgcagg aaaataatga ttctgaaaaa gtgtagctga gattgaagtt
    25981 gaataaagag aaatagatat tatagaagta gtacttaagt acacattata taaaaatata
    26041 ataggtatag gattaaaaag tttagtggtt taaagtttaa ccactaaact tttttaatgt
    26101 ctataatcta ctattattta atttactaaa aataaaaaaa ctcattatat taagataaaa
    26161 atagaaaaat aactagaaaa atactataat atatcagctt aatacagaaa aaagttaaaa
    26221 aatttgagaa aaataaaaat gaaaacgttt tatattgaca tagagaaaaa gtagtgatac
    26281 catttaaata tagtttagta gctaaactat atttaaatta taataactat tttaggggga
    26341 tgttatgaat ttaaaacaat taagtgaaaa atacgaagaa tatgtaattc aaatgagaag
    26401 agaatttcat aagtatccag aattatcttt taaagaaata agaacaaccc aaagaataag
    26461 ggaagaatta caaaaaatag gggtcccttt tgtatctatt gaaccaaata tcgtaatagc
    26521 tactatacaa ggcaaaaatg aaggtaagac aattgcaatt agaggagata tagatgcgct
    26581 tccaatgcaa gaagattcag atgttgaata caaatcagaa tatgatggaa taatgcattc
    26641 ttgtggacat gatgctcatg gagctatgtt actaggactt gcacatatgc taaatgatat
    26701 aagaaaagat ttaaaaggta gagtattatt agtttttcaa gcagctgaag aagttggtgg
    26761 tggacacaag gagataatac agtactttaa ggaaaatgga ggaccagata gattaatagc
    26821 tactcatgtt tggtccgata ttgaagctgg taaaatttct gttgaaccag gacctcgtat
    26881 ggctggtggg tctggttgga gaatagacat aacaggtaga ggaggtcatg gttctagacc
    26941 agatcaaagc atagatccta taaagccagc ttgtgatata gttttaaaag tatcttctat
    27001 accagttaat catataagtg tactagaaca atgtgttgta catgcatgtg caatacatgg
    27061 aggaactaca ataggaaatg ttattccaaa caatgcagag gtaataggtg gatatagata
    27121 ttttactgaa gaatcaggca aaaaagtgtt tgacattata aagcaaatga gtaatggtgt
    27181 tggacaagtt tatggagtgg atgtgaaggt tactcatact ggaggcatag gacctgttat
    27241 caatgatgaa gaagcagtta agatggctaa agaaatagtt tctaatatag atggattgga
    27301 gttagattct tatgagccaa tatgtgcatc agattgttat ggtgaaatat taaaagagta
    27361 ttctggcttt tattgttatc taggggtagg taacaaagaa aaaggaataa tatatgcaca
    27421 acaccatcct aagtacaata tagatgaaga tacattaaag ttaggttgcg aatttatggc
    27481 taaatatgca gttgaatttt taaataaaaa taataactaa aataaatcga attttataaa
    27541 tgggggggat gaaaatgcaa ggtgcattaa aggaaaataa gaaaaaaagt ggtggaggat
    27601 ttttaggatg gattgaaaaa gttgggaata aaatgccaca tcctctagca ttatttacat
    27661 atattacatt gatagtagta gcattatctg caatagcaag ctttttaggt ttttcagcaa
    27721 caagtcctgc tacaggaaag ttaataaaag ttatgaactt aataagtgca aaaggtttag
    27781 tactatttat gcaagagttt gttaagaact tccaaaactt ccctgtgctt ggagtagttt
    27841 tagttatggc agttgccaca ggagtttgtg aaaagacagg atttttttca actgccataa
    27901 aaatgagttt atcaaaagtt aaggggaatg cggttgtatt tataatagct tttataggag
    27961 tatttgctaa tcaagctggt gatgctgctt ttgtattagt accaactctt gcaggagcaa
    28021 tattttatgg attaaataga aatccattag ctggtatatt ctgtggattt gcatcagttg
    28081 gtggtggatt tgcaacagct gtaattcctg ggggatggga cgttacgcta acgccaataa
    28141 ctgtatcagc tgctcaaact attcaacctg catttgatat gacattatta gctagttatt
    28201 atgcactatt tgtatctgcg tttatagtag ctacagtatc aacaatagta acggttaaat
    28261 ttatagaacc taaattaggt aagtatactg gtaaaccaga aggtatagat gataatcaag
    28321 gttttgaggt cacaaaagaa caagcaaaag cagcaaagtt agcaggtttt acagttttag
    28381 cttatgtagc gttattaatt gctttgtgta taccagctaa tagtttttta agaagtccag
    28441 aaggttcttt aatatttgga gcaccactta tgtcttgctt acagtttttt atagtaatac
    28501 tattcttctt gccagggatt gtgtatggaa tgagggttgg taaaatcaag actgtaaaag
    28561 acttaaatga tatattatgt caatcaatgg cagcaatggc accgtttatc gtacttgcaa
    28621 tagttatagg tcaattctta acgctattct cagtgtctaa tttagcacag gtactagcta
    28681 taaaaggtgg agcattactc aactcattgc cactaccacc acaagctata atactgctat
    28741 tcttagtact agtagcgttt ataaatatat ttgttataag cggaagtaca aaatggatga
    28801 tatttggacc agtatttgtt ccaatgttaa tgcaattaaa catacatcca gcatttactc
    28861 agtttgtata tagattagga gattctgtaa ccaatcattt atcaccatta aatgcattct
    28921 ttataatact tttagcaaca gtccaaaaat acgataaaaa tgcaggtatg ggaactatat
    28981 tctcagctat gataccttac actataggtt actttgtagt acttgcagtt ttagtagtag
    29041 tttggatgac aataggtata ccagtaggaa taggtggaca agtttggtta taaaacatat
    29101 ttaataattt tgggaggaaa gaaaatggaa acaactgaaa taagatacaa ttttgatgaa
    29161 gtaatagata gaagtggaac aaactgtctt aaatatgatc ttaagagtca atttatgcca
    29221 gaatctccag aagatgcact accactatgg gtagcagata tggattttgc atgtgcccct
    29281 gagataatta atgcaatgca tgaaagaata gacagaaaaa tatttggata tagctctcaa
    29341 caaactgatg attattatga agctgtatgt ggatggttta agagaagatt tgattggaaa
    29401 atagaaaaag agaatatatt ctttagccca ggggttgtgc ctgcgttagg atttttcaca
    29461 caaatattaa cacaaaaggg tgatggtatc ataatagaaa agcctgtata ctatcctttt
    29521 gaagataaaa taactaataa tgaaagaaaa gtagttaata gtcctttaaa gtttgaagat
    29581 ggtcattatt ctatggatta tgatggattt gaagaattag ctaaagatga aaataataag
    29641 gtgtttatat tatgtagtcc tcataatcca gttggtagag tatggaaaaa agaagagctt
    29701 agaaaaatag tagacatatg taagaaatat gatttgtgga taatatctga tgaaatacat
    29761 tgtgatatta ttcgtaaagg tgaaaagcat ataccattag aagtagcatg tcctgattat
    29821 aaagatagaa taataacttg tacagctcca agtaagagtt ttaacttagc tggtatgcaa
    29881 ttatcaaata tagtaatcaa taataaggaa ttacaaaaaa aatggatgct tgaaacagat
    29941 tctaaattag ctatattcct acctaatcca ttcgcaatag ttgctactaa agttgcttat
    30001 aatgattgtg aagattggat gaatcaagta aatgagtata tagatggtaa tattaaatat
    30061 gtagaagatt ttgttaatga gtatatgcct aaagtaaaga taataaaatc tcaaggaact
    30121 tacttagttt ggttagactt tagagggtat ggattagatg caaaacagct tgaatatgta
    30181 atgcttaaca aggcgaaggt attgctagat gaaggataca tattcggaga agaaggagca
    30241 ggatttgaaa gaataaatgt tgcttcacct agaacaatta tagaagagtg tatgaataga
    30301 ataaaatgtg catttgaagg attataatag taatatctaa ataatatatt tgtgtatttt
    30361 aaaaaaagac tatgctaaaa aaatatttca aagcatagtc ttttaaagtt ttatgatgag
    30421 tttaaaaaat ttcaaaacgt tactattcac ttatatgttt atattagaaa taattccaca
    30481 atatactcat tttctaaagt tgcactttta tacaccttgc ttcaccaata tcatgtgtaa
    30541 ccaaaatagt agacttattc tcattcttaa taatactata aacatcatca caaactaata
    30601 atctagtctg ataatcaaga gctgaaaaag gttcatctaa aagtagaata tctggattta
    30661 cagaaagtgt tctaataagc gcaactcttt gtctcatacc tccagataat tcttttggat
    30721 acatacttct aaaatcccat aatccataag tctttaaaag gctttcaact ctatctaaag
    30781 attttttatc ttttttgcct tgaatttcca atccaatagt tatattatcc attatatttc
    30841 tccattcaag caaattatct ttttgaaaca tatatccaat atttccagta atctttatat
    30901 caccactagt cggttttaaa agattagtaa gaatatttaa tattgtagat ttaccacttc
    30961 cagaaggacc aagcaaagtt aatatttctc cttctttcaa gttaaaattg acatccttta
    31021 atacttcaag ctcgccttcc ttagtgtaga agttcatatt tatattttta acttctacta
    31081 tgttgtcatt cattatgcca ccccctataa cttttagctc gtatatatta tgttattaac
    31141 aatatgacct atttgtacca tttttcataa atataatata aatattttgt ttatttacgt
    31201 attttcttag taacttgtac gtataaatta tagataaata aattataagg taggggataa
    31261 atttgagtaa cataataacg aatttaatag agtcaatatt tactaatcca ataactatat
    31321 tgtttttact tgcaataact gtgtattctg tttttaaaat aatagaaaat aacagagtat
    31381 attcatttgt aagttcaaaa ttagacgaaa tcaatagaga ctataagaca agtgagtttt
    31441 ataaaaaggt aagggatgac tactatacat atagtaaaga aaatccttat gcagacgtaa
    31501 atataacttc atttatagag gaagttgtat ctgatttaaa gcataataat ttgccattgt
    31561 tagaaaaaat tagaagtata aaaaattcat cttcaataag tatattactt ggggttttag
    31621 gtacgtttgt tggtctttct acaatgcttt tatgtgttga tactaaagac attataaatt
    31681 cacttccttc aactattagt agtatgcaaa ctgcatttac aactagtata tttggagttg
    31741 tattttctct aattataggt tactttacta agataaaaga ttgtgaacat gttttgatac
    31801 aaataatgtt aaaatctgaa aatttactaa catctgaaat aactcatttt aagtcagaaa
    31861 gaatggattt aaaagtagaa gaggtaaaaa atacaataaa acaaataagt aagtctatag
    31921 aagctattga aaaatttgat aaaatatcta aagatttaaa tgactttaat gatgaattta
    31981 tcagtggtat agaagcttta aaaagccttt tagaaggttc tcaaagttct attaagacat
    32041 ttgaccagag cgtaagaaaa ttagataaac agtttaatat tttaaatata aaatttgtaa
    32101 agctttttga taaatatgat aatcaagata atataaataa agagatttta tttgatataa
    32161 aagaatcttc aaaaaatata tacaatgcca ctgaaagcca attcaaaatt agagattata
    32221 taaaaaatat aaatgcagga tttgctctat atgaaagaag tgctcaagat ttattaacta
    32281 aattaatgac ccatgaaaat aaaatatctc aaaatcaaaa aatcctttta gatgaaaagt
    32341 ttaccttaga tgactcaata aaaaatctct caagtattat tgaaaacttt tctaatgacc
    32401 tacaagttaa actggatatg atgtttgaaa attctttaga tattcaagat aagttagatg
    32461 ttatgtttaa taactctttt atgaatgatg aagttcctct tgatagtgaa gaattattta
    32521 aggatgatgt taataatgta tttaatcctt ttagtgaaga aatttatgag atagaggata
    32581 aagaaataaa ggtcataggt gaggatgagt taaatgaata ataaatatag aagaactgtt
    32641 aacagagaat ttgaaaagag tagcttttgg ccaacattta cagatttatt atctactgta
    32701 ttgatggttg tgattcttat cttatttagc tcagaaagta tttcaggttc tgttgaacaa
    32761 gatttagcta agaatgtcaa tgcttctgtt gaagaaactt ttaaaaaaag tggaatacct
    32821 gtgaaggtag ataaatctaa tgggcaagta acttttggtg aaaaaaccat gtttgatgta
    32881 gatagtgatg ttttaaaacc agaggctaaa gaaatgctaa aaatgtttgt tccaaagtac
    32941 atagagacta tttataaaga ttatggtgac tatatatcca aaatagttat agaaggtcat
    33001 acagatgatg tgggaagtta tatatacaac ttagatttat cgcaaagaag agcatatagc
    33061 gttgctaaat ttatagtggg agatgaaata ggtgactata aatacaaaga taaagtgact
    33121 aagcatatta ttgcaatagg aaggtcaaaa gctgaactca tcaaaaatgg tgacaatagc
    33181 gtgaatagag atgcatctag aagagtagaa ctaaaatacg aaataaatat taatcaaaat
    33241 aaataattaa atttttataa aaaaaaagaa ggcagttggt gtgagcttta agccttcttt
    33301 aaggaaaaag tttatttatt tttgttgata cttaacatat tatagttttt agaagcagtt
    33361 tgcaatatat taaacgtaaa tggtcgttta aaatgcataa ctggatacta ctgatgtaaa
    33421 atatttcatt ttatataata taaaaattat acagtgtcaa ttttctatat tattgtacta
    33481 taatctatta tttaaaatat tatgtaaaaa aatatcataa aaacgcatat catgttataa
    33541 ttactagagt attatgaata tggaggcgta gtttgtgata gaaataagta taaaaaataa
    33601 aaaaaattct aaaattaaaa ctttgatact tttattgata gtatctatat cgatttattt
    33661 aactggttgt actacatcag ataatggaaa taatcaatca aataataatg attctttgtc
    33721 tagtagtata tcaagtgata agaatgtaaa aattcatttt attgacactg gaaatagtga
    33781 tgcaatattg attcaagatg gtaaaacttt tacattgata gatgggggag ataatgatga
    33841 tgagggatta atggtagatt acctaaacaa tcaaggtgta aaagatatta aatatcttat
    33901 agctacccat tctcatgcag accatttagg tggacttgat tctgttgtca aaaactttaa
    33961 tatagaaaat gtttttgtat cgaatggttc agcagaaaca aagagttata gggactttat
    34021 aaatgcttta gcaaataagg gtttatcccc aagtgtaccg cttgaaaata ataagttcta
    34081 tttagaagat tcttattttg aagttctaaa tacaaatggg ggagatacta ctaatgaaca
    34141 atctctagtc cttgtatata ctaatgggaa tgataaagtt ttatttacag gtgatgctga
    34201 agaaggtacc gaaaaagaaa tactacctaa gcttgagaaa gtagaccttt taaaagtagc
    34261 tcatcatggt tctcgtagta gttcatctca agagttttta gataaagtaa atcctgaata
    34321 tgcggttctt ttagtaggca agggtaattc atatggtcat ccacatcagg agacaatgaa
    34381 taaattaaaa aaaatgggag taaaagtgca tagaagtgat gagtgttcag atataatatt
    34441 tgaatcaaca ggtgatggag tatttacaag ttgtaaagat ggaagttata ataaaggcgt
    34501 tagagaagat ggaaactctg gaaaatcaaa cagtaataca agtaaaaatg attcttttaa
    34561 aaataaacaa aatacatcca attcagaaga aatagtgtat tttactccaa aaggtaagag
    34621 ctatcactct acaaaaaatt gtagtgggct ttcaagaagt aaaaaaatat tatcaggcac
    34681 aattgcagaa agtaagaaaa atgacccatg tgatatatgt tatggaaaat aacaaaaaaa
    34741 caaccaattt atctataaag cttctgaact ttaaatcatt taaaaaataa aataaatgca
    34801 taaaaaaata agctataatc aatagataac acattttatc tatacgaata gcttattttt
    34861 ttaagtattt tttaatagct atttaataac tctaaataca ccagttacaa tacctacaat
    34921 ttcaacttct gagtcattga gcataattgg ttccataaaa tcattttctg gctgaagtct
    34981 tattaaattt ccttctttaa aaaacctctt aaccgtagca gattcaccat ttataagtgc
    35041 aactactatc tgagaattta aggctgtatt ttttttgtct actacaacgt aatcttcatc
    35101 taaaattcct gcattaatca tactttcgcc ttttactctt aatacaaaat tgttactgcc
    35161 ctttactaaa ctggcaggga atggaatgta ttcctctatg ttttgttgag ctaaaatggg
    35221 ttctccagct gttatttgac ctactaaagg aagctctatt atttcttgat tagctccaga
    35281 aacatcattt actttactac gttctaaaac ttcaatagct cttggtttag tagggtcctt
    35341 tcttatgtat cccagttttt caagtttatt tagatgagag tgcacagttg aagtagatct
    35401 taaccctaca gcagtacata tttctcttac agcaggtgga taacctttta atatgatttg
    35461 agactttata aattccaata tcaagacttg cttttcagtt agatctaaat acataacatc
    35521 actcatttcc aaacatattt tgtttttatt tccatacact ttatattttt aattataaca
    35581 taatccattt atatctgcaa acatttgttc ctgattttta atcgtcctca atccattcaa
    35641 aattcttgac tatatcacgt tttacatttt tcttatgagc tgcataaagc tgagtagttg
    35701 tgacattatc atggtcaagt aagttttgaa catcatatat agaaaatcct gtttggatta
    35761 gagaagttgc agccgttgct cttaatttat ggggactata tccattgtct ctagtagtat
    35821 caagtggaat agatgtatat ttttttacca gtgttcttat agcctttgct gtaattctct
    35881 tattttgaag agacaaaaat aaagcatctt tttcttcatc atttaatagt tcatctcttg
    35941 ttctttcgtt ttgcaaataa tcttttatta catgttcaca agtatgattt ataggcatta
    36001 aaacttcttt cccacgtttt ctatatattt taaattcacc tcttgaaaaa ttaaaagagg
    36061 agatattgag ttctctaagc tcatttaatc taagcccgta tgtaacaaat aatgctagta
    36121 tagctttgtc acgtaattta gtttttctcc aatatacctt ttctttctct gtaagccctt
    36181 cacctgtttc tactgattca agcatcttag caacttcatc tatctcaagg cgcttaatag
    36241 catctggctg aggtttagga agttttattg gattgaatcc atcagttata ttattatcta
    36301 tctgagagtt tctgtataag aatttaaata gagtagagat ggatgatttt tttcttgcta
    36361 aagctctatt gttattttca aaaatcaatg tgtttttttc tgtatttttg taatatctac
    36421 tgcaataatc acctaagaat aaatttatat ctcttgattt tattgtatta aaatcatctt
    36481 ctgtaatgtc ttttacacaa tctgcatttg ttaattcttt ggtttcaatc aaatatgaac
    36541 aaaaaaaatt aatatcttct aaataagcaa gtcgtgtaga aactgcaaca gaacctttta
    36601 aatatataaa ataatccttc ataaatacag gaagtttatt ttccaaaagt agacattttt
    36661 ctatgagttc attttctttg ataacaatat tatcaggttt atctgattta ttatgtactt
    36721 ttataatttt cttatcatta ttcaaaatta aacctcctaa aactaaaata taaatctttt
    36781 atattatttt ttgatattta tactaagtga gtataaatat ttaatttaat cattttttac
    36841 atttttaaga gattagcagt gctttagatg aaattatcac taactattcc ctaaatccac
    36901 atttaaagac tagttgaatt taagtactct tttttatttc tcaatatatt tatataaata
    36961 tattgagaaa ttttttatgc tctactagaa tcttttttat ataatatttt tatatgtttc
    37021 taacgaaacc aaatattagt aaaatatgta tttatttaac taaagttttt aaatgggctg
    37081 actaagctta aaatatagga tattatgaga gttactattg aaaaaataaa ccctattgcc
    37141 ataaagaaac ttataaatat gttatactta actattaaac tggatatatt caagttatca
    37201 aatccaaata agtatgtaat tgaacttatt acaaaaataa ctataactat aaagctcagg
    37261 ccatttaagc tattttttaa atcagcacta ctaagtgaca tatgtgtaga tatagaatac
    37321 attaaaaaca taaatataaa atacttcaat gaatatacac taggtgttaa caacattatt
    37381 cttataaaat tggaaatatc acctaaaata atgtgtataa aaccatttat atttaaatta
    37441 ttaatcatgc ttacatataa ctttaaatct atattttgag tcactttcat ataactatct
    37501 ggcagtaata tcttaaaaat taaaataatc attattgttc cacctataat aggtgcaatt
    37561 cctataaaga aatttcctat actttgataa agattatctt ttttatagct atgacttaca
    37621 tatcctagta taccatcatt tttactttct attggtctga aaaacttaac tgaatttatt
    37681 ttatgcatga aaattaaaca cattatcaaa tgactcaatt catgaacaaa tgttcctata
    37741 gctccagtta caataattcc agtttttcca aaagatgaaa atataaattt attatttaga
    37801 ttttctgtaa tgctaaaaat caatccaaat acaataaata ttcctatgaa acaaaaaata
    37861 tttattacac ttatttttaa aatttcgatt atataaatca taagttaaat cctcatgtaa
    37921 aagtatttaa ctactctact tcatatccat tacagcttaa aattttacaa gcattttcta
    37981 tatccttttc ttttactaag atatagtctg tatcataagt agaaattgca aatatgctta
    38041 ttttgttgtc tgctaacaaa ctacttaact tagataaaat tcctacaagt gaaaagtcta
    38101 aaggacctag tatcttaagt attctccact ccttttctga cttaacatca gaaggaatat
    38161 tattatttaa acatacaaca cttaattcat catcagttct agttatagag taaaacttct
    38221 ctgtatctat ccatgttggt attttactgt cattatttaa tctacatact gcatactctt
    38281 ctaccaataa ttttaatttc ataatgtata tttcctccaa tcttatttgt ttacttaaga
    38341 ttatacatta aaagttacat aaaataaaac atataaagta aaaaaggttc actatagtgt
    38401 cctatttcta tagccaacct ttaaaactta attaaattct aataaagaga tatattatct
    38461 aggttcaatt ataagtttaa tggctgtttt cttatcacct tcgatttcta tatcagtaaa
    38521 tgcgggaatg caaactaaat ccattccact aggtgcaaca aaacctcttg ctattgcaat
    38581 tgattttatc gcttgattta gagctccagc tcctatagct tgtatttctg caacgcctcg
    38641 ttctcttagt actccagcca aggctccagc tacagaatta ggattagatt ttgatgaaac
    38701 ttttagtacc tccatttcaa catctccttg ttaatttgtt aaagtatcca ttacctaaag
    38761 tactaaatca taattgccat ttactaatta caatcatcat ttaaagtata cttttataaa
    38821 aatatacttc tataatcgtt ttaagaaaac ctttattttt tttaaccatt ttattatgaa
    38881 aaaactacaa atttctttat attctataag cattttccaa tatttctaaa ttttttatat
    38941 ctttgagcta taatttcctc tttatccata tctttgtaat gatttaatct ttctaaaatg
    39001 taagctttta tttcttttga aacagcttct acatcttttt gtgctcctcc aagaggttct
    39061 tttataatct tatctattat ttttaaatca tataaatcat tacttgtaag cttcataaca
    39121 tcacaagctt ctttagcttt agatgaatct ttaaatagaa tactagaaag cccttctgga
    39181 gaaatcacag aataaactga atgttctaac atacatacat catttcctac tccgatacct
    39241 aaagctcctc cactaccacc ttcaccaatt acaaaagtga ttactggaac agaaagttta
    39301 ctcatttcaa gtaagtttct tgcaatagct tctccttgtc ctctttcttc tgcttctaga
    39361 ccacaaaaag ctcctgatgt atcaataaac gttattatag gtctattaaa tttttcagct
    39421 tgtttcataa gtcttaatgc ttttctgtat ccttctggat gaggcattcc aaaatttctt
    39481 tttatatttt cttttgtatc tcttcctttt tggtgtccaa ctatagtaac attaaacttt
    39541 cctatctgac caattcctcc aaccatagaa gggtcatctt taaaaagcct atctccatga
    39601 agttctaaaa aattagaaca aatatgttct atgtattcca atgtcgtagg tctctttata
    39661 tctctactta atgttacctt ttcatatgct gatagatgtg aaaaagcatc ttccttaagc
    39721 ttttccaatt tctcattaag tgaatttatt ttatcactta aatctatatt atttgcctta
    39781 gaaatagtta ttaattgttt aatatcattt tctaagactt ttattttttc actattattt
    39841 tcaatcaaaa cagttcctcc tttatgccct atttcttgtg catagcaaga atttgatata
    39901 atacttcctt catttttctt ctatctacta tcatgtcaat aaaacctttt tcaagtaaaa
    39961 actcagaagt ttgaaatcct tcaggaagtt tttgatttat tgtttgctct attactcttg
    40021 gacctgcaaa acctatcaac gtatttggct cagaaattat aatatcacca agcatagcaa
    40081 aactagcagt tactcctcca gtagtagggt cagttagaac tgatatatat ggcaatgatt
    40141 tttcttcaag cttagaaagt gcttgagaag tctttgccat ctgcataaga gataccatac
    40201 cttcttgcat tctagctcct cctgaagcag aacaaataat tataggtaaa ttattttcag
    40261 cagcatactc tattgaataa gttatttttt ccccaacaat agaacccata cttcccatca
    40321 taaaatcagg attcattaca caaataactg ttttaattcc atttattctt ccataacctg
    40381 tgacaacagc ttctgactct tttgtttttt ccttataaga gtctactttt tctttgtatt
    40441 taggaaaatt tagtggattt tgaaattcta catcagaatt aaactccata aaagttcctt
    40501 tatcaatcaa taaatttatt ctttttcttg ctcttaagcc ataatgtctt gaacattttg
    40561 gacatacaaa tgaattttct tctatgtcct ttctaaatac atgactatca caacctttgc
    40621 aataggtcca aaatttatca tctacactat tctttttaaa gttgtcatct aaagaaatcg
    40681 taacatactt agattctttt gatgataaaa atttttttat cataatctat ttactcctta
    40741 taatgtagca aattctttct tcataaagac atcttctata aatttggtat gatgagtatc
    40801 ttctctaaac tcctcacttg ttacaagtgc cttttgaaat tctacatttg tattaatacc
    40861 ttctacaata atttcgtcta gagctctata tattctactt atacattctt ctctagtttc
    40921 agcccaacat attaacttac ctatcataga atcatataaa ggtggtatag tatatcctgc
    40981 atatacaaaa gtatcaaatc taacaccaaa acctccaggt aaatttaatg attctatctt
    41041 acctggtgat ggagcaaaat tctttgatga atcttctgca tttattctac attcaatagc
    41101 atgacctcta aaagtaatat cttcttgttt aaaaggaatt ttatccccat tagctataga
    41161 tatttgaagt tttataaggt caagatttgt taccatctct gtaactggat gttcaacttg
    41221 tattcttgta ttcatctcta taaaataaaa gtttttatct ttgtcaacta taaactcaat
    41281 agtacctgca ccgatataac ctactccttt tactatatct actgcaattt tatgaagttt
    41341 ttgtctttct tcatctgaaa ggtatggact taaagattct tctattactt tttgatttcg
    41401 cctttgcata gaacaatctc tatcacctaa atgtattgca tttgaatgtt tatctccaaa
    41461 tacttgtact tctatatgtc taggattttc tataaacttt tccatatata aatcactatt
    41521 agaaaatgct gctagagctt ctgaacttgc catatcaaaa tttgaaaaaa gttcttcttc
    41581 ttttctaacg attctcatac cttttccacc gcctccactt gaggctttta tcataacagg
    41641 atatccaatt tctcttgcaa cttctaaagc ttcttcctta gttttcaaaa cagattctga
    41701 accaggaaca acagggatat tcaattccat catagtttct cttgctctag atttatctcc
    41761 catcaaaatc atttgttctc ttgttggacc tataaaaata atattattat cttcacattg
    41821 ctttgcaaat tcaggattct ctgataaaaa tccaaagcct ggatgaatag catcacactt
    41881 taatttaaga gcaagataga ttatattttc tatattatta taactcttac ttatattatt
    41941 tggtcctata caaatagatt catcagctat atatctatga aaacaatctt tatctatttc
    42001 agaatagata gcaacagttt ttatacctag ttctttacat gttctgatga ttctaactgc
    42061 tatatcgcct ctatttgcaa ctagtatttt ttttatcaac ttaaatacct cctactacaa
    42121 tggttttatc ttaaataaag gttgattata ttctactagt tcttcattac taactaatac
    42181 ttcaattatt tcacctttaa tttcacttgt tatctcattc ataagcttca ttgcttctaa
    42241 tatacataaa gtgtcgcctt cttcaactac atcgccaact ttaacaaagc tatctgcatc
    42301 tggacttgga gaatcataga atgttcccat caaaggagct actactactg atagattctc
    42361 attagatgtg acaggtgtat cgtttacaac tggcttttct aaagaaagat ccactactga
    42421 ttcttgttgt acacttaaaa caggtgaact tgtagattga gcttttttag atacttctaa
    42481 tcttaaatcg ctactttcaa gttttacata ttctaagtta gttgagtcta tggcctttaa
    42541 taattcctta atttcattga tgttcatttt gttaccccct tgtattatta cctggtgcta
    42601 atattatatc acaaaaaaca aatttataaa agaaaaagtg ctgactagag aatcaacaca
    42661 tcaatatatc ttttaatttt aaattaacaa ccagtaactt atatttgttt taattttcca
    42721 ttattaatat aaattataag tataaaatga ataaaattca atattttttg caaaatacaa
    42781 gagaatacaa attaatacct cgggaatttt taagacaaat accaatatta tgcaatttag
    42841 acttcgcttt ctttcaaatt agtatcattt tctttatttt tacataggat gtactttaat
    42901 ttagctatca aaacattgct atataggtta aaagttatta gaattgcagg aattataaag
    42961 taaactctag cacctataaa accaaaaact actgtaataa gtattaatct tttatttaaa
    43021 gtaaaaaaag gtatattaaa aatacttttg tcggatgttt ctggagtata agaatatata
    43081 actccaaaaa tcaaagtaag catacaaagt ggaagtagtg tataacttga aattattccc
    43141 aaaaatccta tactttcaaa atatttgcta gcactatttg tataaacaaa catgtatcca
    43201 tttaaatatg cgcatataca tgatataatg ccaaaagttc cccctaagga ttgagtgtac
    43261 caattaaaaa atttagattc catatacctc acctcctaaa actgtatata taatgatatg
    43321 catttaaata gtaaaatata tctttatatg tttctgaata aaattttgtt aatttcatta
    43381 atttatgttt aagaaatttg aagtatagtg tagtatatat taatgtagat gaagttttat
    43441 agtacttatt tcatagtgct taatcaagtt atcttattat ggattcaaaa gttgtttgta
    43501 gggttttata ttttgttgag ctgaaaaatt tattataaat gaagggatgt tttcgctttg
    43561 gtagatacat tagaatttgg tcttaaaatt ttatttttta tattatcaat aatttggatg
    43621 ggtaaaataa tgattttaag aactgataag caaatagtaa tcaatccttt gcttattggt
    43681 atatctgcaa tccttgtgat gttgcatacc tcacagagta acattgaatt ttttggatta
    43741 gatgtccaat atataagaat agttttgtat ataatatatt cactgataat tttaatagga
    43801 atatgggcta ctaacagaag aaatggaatt ttttagataa tctatttcta taaaatttaa
    43861 aggttgctag taacaaatca ctaatattag cgttctgtta ctaacaacct ttattttgat
    43921 tatacactta aattgaaaat agaatgattg aataagttga cagattattt tacattgaca
    43981 gtaattagaa cttcttgaga tgtatttaaa tcatttttgt cgcttagtat aacttttacc
    44041 actgctttac ctgttttatt acctttaatt atcatatctt cacatgaaag tacactctta
    44101 tcacttacag atacaataaa atttaaatct tttggaaggt tatcagtaac ttcccagtat
    44161 ttataataac tatcaatttt cttcgtaatt atttttctca aatcaatact ttcacctttt
    44221 gctatatcaa cattatgata tttagagtcc agtttatact gattatcttt tgtatcaata
    44281 aaatttaaat cgcaatttac atccacacca ttatgagaaa gagaatttac tacatcgtca
    44341 tcaatcaaat aattattggc aagatttatt gaatccatat cttttagact taaagcttcc
    44401 ttaggaacct cttttatatt attattccaa aaaataaaat tgtctacatt ttcaagatat
    44461 gatatacctt ttaaatttga aatactctca ccagttgcat aaaattctcc atctaaagcc
    44521 tttatattgc ttatagtcat ctcatctgga tttatatatc tttttacaac tgctcttaaa
    44581 tttgcatcag gaaaaatatc tttaagttga ttttcgctca aaaccttatc atttgaattt
    44641 attggactta aagctgctgc agaaataata aatggtatta aattagctaa attcatagta
    44701 ccctcccaca taaatttttt attataataa tttaatttct atgagagggt tctgtttcct
    44761 tgttatatat acctactttc gtacacttta ttaatataaa ttaaaataaa tttctactat
    44821 taaacttata aacatattac tagttacgtt ttttagcttc acatctaaat attcgcatac
    44881 cttgtgacat aaatttgtct tcatactctg ttgtaacatt attttcatac tcactatttc
    44941 ctaaatcaag agaaatattc tttaatagcc aattatttgc agcaatttca tttaaactaa
    45001 actcgaataa tttttcatta tctgttttaa agtgaatttc gccatcatct tccaaaaccc
    45061 tattgtacat ttctaaaaat ccagaatgag tgagtcttct tttactccat ctatttttag
    45121 gccatgggtc acagaaattt atataaattc tcgataattc tttagcttca aagtaatcca
    45181 aaatattgct tacatctccc cataaaaata gtatattatt caaattagaa gcatctgctt
    45241 tttcaacagc ttttaaaaga acttcttcct taagttccat ggcaatataa tttatgtcag
    45301 ggttctgctt agctaaagtt gttataaact taccttttcc agtcccaaat tcaacatgta
    45361 ttggattatc atttctaaac tttaaattcc acttgccttt taatttgtct atatcgtctc
    45421 ttaaaacata ttttgtataa cttaaaagct tttcatctgc accttttttt cgtcttcttc
    45481 tcacaataat tctccttaca tataaaaaat tagtaaaata catttttatt atacattatc
    45541 tcaccaaaaa ataaaaagaa ctctcttaaa atagaaattt atttctataa agagagttct
    45601 aaacttacta aaaacttaat cttagtggca ccctgaacaa ccagagcatc ctcctccacc
    45661 ttgatctttc atattttcag gaataactct aaatccaaat cctgtatctt ctattattat
    45721 atctccatac tgactatatt catcagagct cattacaaat tgtaattctc caacttcata
    45781 agttaagtca tcttcttttt tctcatctaa agctaatcca aaagaaggac ctccacaacc
    45841 aacacctgca aagtatactc ttatattatt aggtttatct tgattgtctt ttaagatact
    45901 ttttaaagta tcaatagctg tttctggtaa agtaattttc atcaaatcat tccctttcaa
    45961 atattttatt aaaacaatat ctattatata cccatacaag gtacataatc aattagtttt
    46021 tgcatatttt actaaaactt ctacctaata ttacctaagg ctttaaaata ttttatataa
    46081 tattttatta ccttaaataa ataccctat
//
LOCUS       DAMJVK010000022        45491 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.022, whole genome shotgun sequence.
ACCESSION   DAMJVK010000022 DAMJVK010000000
VERSION     DAMJVK010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 45491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45491
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.022"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            93..752
                     /gene="cprR"
                     /locus_tag="PC414_001215"
     CDS             93..752
                     /gene="cprR"
                     /locus_tag="PC414_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CprR"
                     /protein_id="HDF2463620.1"
                     /translation="MSKILVVDDELDMLKLIENVLKRDGHEVKVISDVEKAMNMDFNY
                     FNLIILDVMMPKMDGFEVCKTIRNKVDCPILFLTAKNMESDIMYGLGIGADDYITKPF
                     GVGELRARITAHLRRESREKKNLLTISNVQFNFLGKEVSIDGKKINFTKSEYLICEFL
                     AKNKGQVFSKERIYESVYGFDGESDISTITEHIKNIRSKLKQYDIYTIETVWGIGYKW
                     V"
     gene            963..2021
                     /locus_tag="PC414_001216"
     CDS             963..2021
                     /locus_tag="PC414_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HDF2463621.1"
                     /translation="MDRLKNIRKSLLQYTFLFLLIGITIYLVFKTLDIKMLSNVMVMV
                     DKKFLFIGALAIMCHIMLEGVVMKIIIESTHKVNIRFIGFKLAIMGFYYNLITPFASG
                     SQPVQIYVLKKCKMPLSKASAVVTNKSIIFQIVVTFYCTILVLMNFAMLEKQMKMIMP
                     LVFLGIAINSFVLIMIILVILNPEKIKYFMRITIKHLSKIKFLKFLDNKIESIESFID
                     DYSKAINFFAKNKKVLISTIIVTFIQLSAYFSVSFWIYKAFNLQGYSYIYILTLQAFL
                     YMAISPIPTPGNVGANELAFFTIFKSVFPQPLMGYAVFLYGGFMYYLILIGSGIFTVI
                     THYRMKNRIGRNIVLSEN"
     gene            2272..4416
                     /locus_tag="PC414_001217"
     CDS             2272..4416
                     /locus_tag="PC414_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:2686..2688,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase H subunit alpha,
                     selenocysteine-containing"
                     /protein_id="HDF2463622.1"
                     /translation="MEKKILTVCPYCGAGCQLYLVVKDNKILRAEPANGRTNQESLCL
                     KGRYGWDFLNDPKILTPRLKKPMIRKQGVLEEVEWDEAINYTASRLNEIKEKYGPDSI
                     MGTGSARGPGNEANYIMQKFMRATIGTNNVDHCARVUHAPSVAGLAYSLGSGAMSNSI
                     PEIENADVLFIFGYNGADSHPIVANRIVKAKKNGAKLIVTDPRVTESARIADIHLPIK
                     GGTNMVLVNAFGNVLIEEGLYNKEFVQNHTQGFDEYKEIVKPYTAKYAEKITGIPEEL
                     IRKAMREYAKGKKAMILYGMGVCQFGQAVDVVKGLASIALLTGNFGRESVGIGPVRGQ
                     NNVQGACDMGALPNVYPGYQNVTDDKIREKFEKAWGVKLSPNNGYSLTQVPNLVLKEK
                     KLKAYYIFGEDPVQSDPDASEVREALDELEFVIVQDIFMNKTALHADVILPATSWGEH
                     EGVYTCADRGFQLMRKAIEPQGDVKPDWQIISEISTAMGYPMNYKNTKEIWDELRQLC
                     PSFLGATYEKIETQGCVQWPCKSESMEDKGTMYLYEGQKFSTPNGKGNLFAAEWRPPM
                     EVEDDEYPFSLCTVREVGHYSVRTMTGNCRTLSSLEDEPGRVQINSNDAEKLGIEDDE
                     LVRISSRRGSVITRATVTDRVKEGATYMTYQWWVGACNELTIANLDPISKTPEYKYCA
                     VKLEKLEDQELAEKCVREEYQSLKDKMTATNI"
     gene            4465..5298
                     /gene="fdhD"
                     /locus_tag="PC414_001218"
     CDS             4465..5298
                     /gene="fdhD"
                     /locus_tag="PC414_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HDF2463623.1"
                     /translation="MNNIYSKTKLQENSKDKVFENSYSLVDYKYLNSFNVEVSKINEH
                     EVSIDNEEIIAEYPLSFILNDKYVNTFLCTPYNLEELIVGFLKTKGYIENKKSLLKFE
                     INESERFAKVTIQEKQKNQDEQIIFLNSLDYITCAPVKSNIKINYDTIYSIMDKNLNS
                     SELFKNTGGVHSVSVFNFDKKIVTCEDVARHNAMDKAIGFCVLNDITLKDKIVAVSGR
                     ISLEMILKAAMTQIPIVVSKSAPTNLSIELSKKLNITLIGFVRGRRMNIYANPQRVIK
                     K"
     gene            5309..5482
                     /locus_tag="PC414_001219"
     CDS             5309..5482
                     /locus_tag="PC414_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463624.1"
                     /translation="MCTKKEEQRYSKCEICGVLIDDTDKVYNNTYNLCKKCKRNISTE
                     KIVKYINIYDESK"
     gene            5540..6091
                     /locus_tag="PC414_001220"
     CDS             5540..6091
                     /locus_tag="PC414_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF2463625.1"
                     /translation="MKNKIGSFVIADPNKCTGCRACEVACFTMHNQHNNVGYTVGTVD
                     IPVIPRLYLVKGDNFCMPIQCRHCEDAPCLNSCPQKAIVKENNIMSVNEEKCIGCKTC
                     LLACPFGAIDLLPQYEDGKEVEQVILDENKKIAYKCDLCKDNEKIACISACPQEALKL
                     VTPIDDKKAKNRQAALSLLLTNK"
     gene            6116..7492
                     /locus_tag="PC414_001221"
     CDS             6116..7492
                     /locus_tag="PC414_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HDF2463626.1"
                     /translation="MIINIDKDLCTGCRECSKVCPVNAIEGEEGKPQEINLDRCVMCG
                     QCVQTCKSYASVIDEGFEFLQEKKQEREIPESINEPIFAAYNVCDIDKVKKALSDPNV
                     FTMVQCAPAVRVALGEDFGYYLGYLGAGKMAAALRALNFDRVYDTNFAADLTIMEEGS
                     ELIKRVTEGGTLPMFTSCCPAWVKFMEKNYPKLTNHLSSCKSPQQMGGAIFKTYGAEI
                     NNVDASKIFSVSIMPCTCKKYECDREEMDSSGYRDVDVVLTTRELAYLIKDMGIDFKN
                     LREEKFDSPLGSYSGAGTIFSVTGGVMEAAIRTGYELITGESIPDVEVKQVRGENGFR
                     KSTIKIGDLDLRVGVVSGLKNVIPVLEDLEKGKLDVDFIEVMTCPVGCVSGGGQPKIL
                     LEEYKELAYENRTKATYVHDEHLAIRKSHENPDIKKLYDEYLVEPLGEKSHHLLHTTY
                     CIGKEVAK"
     gene            7492..8007
                     /locus_tag="PC414_001222"
     CDS             7492..8007
                     /locus_tag="PC414_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF2463627.1"
                     /translation="MNYFVIADPDKCIGCRTCMIGCVVAHSDEDIFYQNPDEINFNPK
                     LSVIKTKEVSAPIQCRHCEDAPCAKACPNGGIVRVGNTIKINEENCIGCKTCMLACPI
                     GAIDIVTLKDVDEGKLCFRERMTANKCDFCIESPEGPACVNVCPTKAFTIVKDEDIDS
                     KVKNKRKLAIL"
     gene            complement(8085..9368)
                     /locus_tag="PC414_001223"
     CDS             complement(8085..9368)
                     /locus_tag="PC414_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugated bile salt MFS transporter"
                     /protein_id="HDF2463628.1"
                     /translation="MNTLLNNKFSIKNFSNAWVIVLGCMLIQAVPFSIVASIQPQFIS
                     YVVEGEGFSLASFSLIFTIGTIASAIASPFIGVLFSKVNIKILYLLGCIISSIAFATF
                     SICTELWQFYTVSCIVQIGATIISAIGIPVLISAWFDEESKGKALGFAFAGGSIGSIF
                     LQPLAVRLLATQGYSRSYLIFGIIALIVGIPVILFILRMPKDINEKVKSKGKKEKASD
                     TISPSVWGYTLKEAKKIKYFWIMTIGFIFVGIYVSSLSVQYPTYLRNYLNPILIGTVG
                     STFALFALIGNLFGGLLFDKFGQTKPLIISFVLVLIATLSLLLVDKSSTFAFVFAILK
                     GLSVFAYMIGPAYLTGSFFGQKEFGSIYGIVQLIFAVGFALGSTLFGLLVDNFGYRFS
                     WSIIILSVIIAYTLLIITSIGMKKLNKERLDKLMQ"
     gene            9556..9978
                     /locus_tag="PC414_001224"
     CDS             9556..9978
                     /locus_tag="PC414_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3783 domain-containing protein"
                     /protein_id="HDF2463629.1"
                     /translation="MSFEKINNNNLDKIHNRKCIMLVNFNKKELSLIKNVSNFAGIRD
                     HIILSDKNGENIIRDILDGNISNECENGIKSKSVIFNNTPSTQVNAFLEGLKKVRINR
                     PFTAMVTETSIDWTLNNLIENLVQERNAMSLGKTIEHK"
     gene            10080..11018
                     /locus_tag="PC414_001225"
     CDS             10080..11018
                     /locus_tag="PC414_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDF2463630.1"
                     /translation="MKVLFTINYGKEKFDKIRELGYDVIHYSEDSIENNEDVNTADVL
                     VTYNPFERLDISKMKNLQYIQTTSVGIDQLPKEEVLKRNIKIANNRGGYSVPIGESIV
                     LYILEIYKNSAKFFKQQQNKEWKMDFSVSELYGQRVGFLGTGTISSEAAKRLKAFGVE
                     IWGVNTDGSSKKYFDKCFATEDMNIVFEECDIVVVAMPSTKSTDGIVNKDMFKLMKDG
                     SVFINVGRGNTVNQKDLEECVEKFRGVALDVFESEPLNKDNKLWECENVIVTPHNSWV
                     SDKNKERTFNMVYNNLKHYIMENKPVDNVVDISRGY"
     gene            11204..13228
                     /gene="ligA"
                     /locus_tag="PC414_001226"
     CDS             11204..13228
                     /gene="ligA"
                     /locus_tag="PC414_001226"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDF2463631.1"
                     /translation="MSVKDEINKLIETINYHNERYYNQDSPEISDYEYDKLMKELISL
                     EEEHPELKRIDSPTNRVGGKPLDKFNQIVHKTPMLSLSNAFSEQDLRDFDKRVQEYVG
                     ENVEYVVEFKIDGLSVGLTYNNGEFEKGATRGDGVVGEDISQNLMTVKSIPLKINDKK
                     ELIVRGEVYISKDNFRKVNEQQEEQEQPLFANPRNLAAGSLRQLDPKLTAKRPLDIFV
                     FNMENIEEYDLESHSESLEYLEKLGFSVSQSYKVCKSIDEVIEHIEYWTDNRGNLDYE
                     IDGMVIKVNNIKQRDEMGYTAKSPRWAIAYKFPAEKKKTKIVDIIVEVGRTGTITPSA
                     ILEPVRLAGTTVSRATLHNEDYIREKDIKINDTVLVQKAGDIIPQVLEVIKEERTGDE
                     IDFKMPEKCPVCSEPTIRLEGEAAVKCINMSCPAQIRRGIIHFVSRDAMNIDGLGESI
                     ITLLLNEKIIQDVSDLYYIKKEDVVDLERMGEKSADNLINSIEKSKENDLWRLINGLG
                     IKFIGVKAAKILAYNFKDLDEVISATSEQLEELEEFGSIMANSVVEFFKEDKNMNVIN
                     KLKNVGVNTKSLDNTGEKVEKIFEGMKIVLTGTLPTLKRNDAKDMIEKRGGKATSSVS
                     KSTTFVLAGEEAGSKLTKANDLGIKVIDEERFLELLKLSSKDEVKAVLES"
     gene            13388..14737
                     /gene="rph"
                     /locus_tag="PC414_001227"
     CDS             13388..14737
                     /gene="rph"
                     /locus_tag="PC414_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HDF2463632.1"
                     /translation="MTRSYDRSNEQIRPVKITRDYTKYAEGSVLIEMGETKVICTASV
                     EEKVPPFLRNSGTGWINAEYSMLPRATQQRKIRDASRGKIDGRTQEIQRLIGRTIRSV
                     VDLNKLGERTIWVDCDVIQADGGTRTASITGAFVAVAEAIYKLYKAKTIKEMPITNFV
                     SAISVGIVNGIHMLDLCYEEDSKAQVDMNIIMTDKCEFVEVQGTGEEKPFSRADLNLL
                     LELGEKGNKELIRLQREALGEISDEILGMDYGNEVVIATNNAHKLEEIGEILKDFEYK
                     VYSLKDVDLAGIEIVEDGKTFEHNALIKARTIAKKTKLIAISDDSGLEVDALGKKPGV
                     YSARYAGEHATDEENRKKLLKAMQNVPMSKRNARFVSAIAVVFPDGKEFVVRGICEGM
                     IGFEEKGKNGFGYDSLFIVKGYDKTFGEIPSVIKNSISHRANALKLMKQEFIKRVVK"
     gene            14737..15207
                     /locus_tag="PC414_001228"
     CDS             14737..15207
                     /locus_tag="PC414_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF2463633.1"
                     /translation="MKIGIVSDTHMMIKNMEKTIPYLKECDLIIHAGDNFTDSRYIHS
                     MTNVGIIAVKGNCDFDAVEEEVVFEVANKTIFLCHGDKYGVKYGTNMLEKKATEVDAD
                     IVIFGHTHTPFREIKDGVLYINPGSTSLPRGVSYKSFVIMDIEEDDIKIEEIRI"
     gene            15401..16687
                     /locus_tag="PC414_001229"
     CDS             15401..16687
                     /locus_tag="PC414_001229"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HDF2463634.1"
                     /translation="MKAELIKKEGNKVTLKITVDNNKFEAAVNKAYNKSRNKYNIPGF
                     RKGKAPRIVIETQYGKGIFYNDAIEILFPEVYPEAIKELDIDPIDNPDLDIEEISKDN
                     GLVMVLNVEVKPEFELGNYKGIEIAKVENTVSDENVDAKLQEMVEKNARLVSVEDKAL
                     EDGDTAIIDFEGFENGVAFDGGKGENYNLVIGSNTFIPGFEEQLVGKKAGEEVEVNVT
                     FPEEYHSQDLAGKPVVFNVKINDVKVKELSALDDEFAKDTSEFDSLDELKADVRAKLE
                     EEAKNRADAETRNSVVEKVAENTEIEIPEVMIQHQIDNMLNELNYQLQYQGFGLQQLL
                     EMTGRTMEELREERKEDAKKLVKSSLVLEAITKAEGIEATEEEFKAELEKMASAYNME
                     VEKIEASLRDADKEDIKGQIKIRKTIDLLVDNATIA"
     gene            16837..17421
                     /gene="clpP"
                     /locus_tag="PC414_001230"
     CDS             16837..17421
                     /gene="clpP"
                     /locus_tag="PC414_001230"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDF2463635.1"
                     /translation="MALVPVVVEQTGRGERSYDIFSRLLKDRIIFLGDQVNDATAGLI
                     VAQLLFLEAEDPDKDIHLYINSPGGSITSGMAIYDTMQYIKPDVSTICIGMAASMGAF
                     LLAAGAKGKRLALPNSEIMIHQPLGGAQGQATDIEIHAKRILKIKETLNEILSERTGQ
                     PLEKIKMDTERDNFMSALEAKEYGLIDEVFTKRP"
     gene            17440..18690
                     /gene="clpX"
                     /locus_tag="PC414_001231"
     CDS             17440..18690
                     /gene="clpX"
                     /locus_tag="PC414_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HDF2463636.1"
                     /translation="MSKYEEKRQLKCSFCGKNQDQVRRLIAGPNVYICDECVELCDEI
                     IQEEIEDTIDEDTTSLPKPKEMMEILNDYVIGQEKAKKALSVAVYNHYKRIYSKKSSS
                     KDIEIQKSNILLLGPTGSGKTLLAQTLARTLNVPFAMADATSLTEAGYVGEDVENILL
                     KLIQAADFDIEKAERGIIYIDEIDKIARKSENPSITRDVSGEGVQQALLKILEGTVAN
                     VPPQGGRKHPHQEFLKIDTTNVLFILGGAFDGLEKIIQKRGGDKTLGFGAKIESKKEL
                     DLGKLYEKVLPEDLLKYGIIPEFIGRIPVLATLELLDEDALMQILQEPKNALVKQYKK
                     LLELDDVELEFEEGALRAIAKKAIERNTGARGLRSIVESVMMETMFEVPSRDNIKKVI
                     VTEKSVNEDSVNPIIVLKDQEESA"
     gene            complement(18761..19288)
                     /locus_tag="PC414_001232"
     CDS             complement(18761..19288)
                     /locus_tag="PC414_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HDF2463637.1"
                     /translation="MSEIILLFLIFLKIGAFSFGGGYAMLPFIQQEFISKYHFITNQE
                     FLDLLAISQSTPGPVAINSATFVGFKTAGFFGSLAATIGVTIFALIGLTIISKLFSKF
                     KNNKKVENLLTVLRPITLGFILSAAFSSAKEVNWDLYGIIAFIASFYLLYKGKIGSIA
                     IVFVFGILGILLTFL"
     gene            complement(19276..19788)
                     /locus_tag="PC414_001233"
     CDS             complement(19276..19788)
                     /locus_tag="PC414_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HDF2463638.1"
                     /translation="MLKLFLTFLKIGAFTFGGGYAMVPLIQDELVNKQKLLEQEEFID
                     YLSIAQSYPGVLAVNISVLLGYKLYGIPGVLICTLGSALPSFCIVLVLSYLYFKNSSS
                     KILDGFFKGVAPVVIALILYSFTSMFKKLPKSKTNILLLIIAVLSVGVFNISPIYLII
                     GGGVYSLCQK"
     gene            19968..22331
                     /gene="lon"
                     /locus_tag="PC414_001234"
     CDS             19968..22331
                     /gene="lon"
                     /locus_tag="PC414_001234"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HDF2463639.1"
                     /translation="MEQNYTKIDHELPLIPLRGLAIFPYMILNFDIGREISLKALDQA
                     MMDEELIFLTSQKEAEVDEPGEEDFYHVGTICKVKQMIKLPGDTVRVLVEGVSRGRVK
                     KIEQEDGYFRAVIEEIVFDSDNLDSETEVEIEAFVRNVFDAFEEYINIGNRVSPEILI
                     SLADIEDVDRFIDTIAANIYLKSSQKQEILEEFDIRKRLELIYSILLEEIDILKIEKK
                     ITLRVKKQMNKVQKEYYLREQLKAIQKELGEEEDINSEADEYREKLKKIKAPKTTKEK
                     IEKEIDKFSKISSMSPDVSVSRNYLDTIFSLPWNKETKDKLDITKAKDILDEDHYGLE
                     KVKERILEYLAIRTLAKSLKGPIICLVGPPGTGKTSIVKSIARALNRKFVRISLGGVR
                     DEAEIRGHRRTYVGSIPGRIINGVKEAQTKNPVFLFDEIDKMAADYKGDPASAMLEVL
                     DPEQNKDFVDHYLEIPFDLSKILFVTTANSLSNIPRPLLDRMEVIEVSGYIEEEKLNI
                     AKKYLLPKQIKEHALKENFIKIDDETLRSIINHYTREAGVRTLERTIGKICRKVAKKY
                     VEDPTLEEVVINKSDLETYLGKDMFKYQLAEVNPQIGLVNGLAWTEVGGVTLEVEVNV
                     LKGKGEIVLTGKLGDVMKESAKTGISYIRSIVDKFDIDPEFYKTNDIHIHIPEGAVPK
                     DGPSAGITMALAVISALTKRPVPGNIAMTGEITLRGRVLAVGGVKEKLLAAHRAGITK
                     VLIPKECEADLDEIPENVKEKMEFVLVEHMDEVLEQALLKSGENNEN"
     gene            22321..22932
                     /locus_tag="PC414_001235"
     CDS             22321..22932
                     /locus_tag="PC414_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HDF2463640.1"
                     /translation="MKIRSSEITMSAVNKSQYPAEGIPEIALAGRSNVGKSSIINTLL
                     NRRNFARTSQTPGKTRTINFYLINNEFYFVDLPGYGYAKIAKSEKEKWGGIMERYLES
                     RQELCSIFLLVDIRHEPTADDKLMYEWIKHFGYNCVVIATKADKISRGQYQKHISIIR
                     KKLQMESSEKVIPVSSLKKTGVEELWEEIVNQYNQHGYEITVD"
     gene            complement(23197..24567)
                     /locus_tag="PC414_001236"
     CDS             complement(23197..24567)
                     /locus_tag="PC414_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2463641.1"
                     /translation="MKQKWIVLIIICIGVFMSTLDGSILNIANPTIAADFKINMSQIQ
                     WVVTAYMLVVTATMLFFGKLGDKVGSNRLYTLGFFIFTIGSFLCSMSNNLSTLISSRI
                     FQAVGASILMATGLGIVSNAFPANEKGKAIGITGAVVGIGNMSGSVIGGIILEHFGWP
                     SIFIINIPIGIIAVFLGIKFLPKPVLDEQNKSFDIPGLLLFASCTTLILLAMNEKGNT
                     RLYLGITALIIFLLLALREVKFEQSFIDLPLFKNRNFTVGNIIGVACYFPQMAVSFLL
                     PFYLEQLKNLSPMMAGYVMTVHPLIMVLIAPIAGSLSDKHGAKNILTASFSFMTISLV
                     GMALLKADSPLYLLIVCLVIFGLGLGAFSSPNNSSILADVPPQKQGYGGSFLATIRNL
                     SFALGTAFFSSFFAQSLTYNQKFKSHTSAYVIASNQSYWIAASVCFIGLILTVFFMRK
                     TDKSIS"
     regulatory      complement(24742..24857)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            25145..26224
                     /locus_tag="PC414_001237"
     CDS             25145..26224
                     /locus_tag="PC414_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2463642.1"
                     /translation="MEGKVKKAFPGGNTSQGSYSFFDYIIPENVKRVFCLKGGPGVGK
                     SSLMKKIYKEFLHRGYDIDLYHCPSDPSSLDGLVIKKLGVVLMDATAPHTMDPKLPGA
                     LDEIINLGDYWNTEELEKNKQEISVYDKDISNSFKRAFKFLKSAEPIFRDIEEKYSDC
                     MDYGKVNLVTEEFIKRLFDGVKSTGNLKREKHLFGSAITPVGCLDYTENILKDVKKVY
                     YLDGEIGTGKTTLLNKVYKKAIEKGLNVEVYHYPLIPEKIETVLLTDLDIGITISTTF
                     KEEDTIDLNQFLNREKLLKYEEDIKFDEKVLDDLICWAVLNLKRAKSKHDIIESYYSP
                     NMRFDEVGKLRDKLIKRILKYEENL"
     gene            26615..27295
                     /locus_tag="PC414_001238"
     CDS             26615..27295
                     /locus_tag="PC414_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HDF2463643.1"
                     /translation="MKKQYVFIILASTILGILVGTIFRDYSNEETVYTSKDKSIRKEI
                     KMTRKSIKELTKEKDKLDKEINSMKQNQKNSEEISEIETLRDSLSYSDVKGSGIILSI
                     DAIDEKTGNIANSIDYNKILVKVINELKVNGGKYIEINGQRINQYSEVVLAGSHININ
                     GVPVAQPYEIKCIGDKEKLSNYVDKGSKYLDSIQTNYPMKISIKTEENISMKKMNIPN
                     KLKYIKGD"
     gene            27288..28667
                     /locus_tag="PC414_001239"
     CDS             27288..28667
                     /locus_tag="PC414_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HDF2463644.1"
                     /translation="MIKIILEESSLPMLIPEKLAKKYNVFPVEFQDDNLVVEIEDEDI
                     YALQDLRLATGKEIILKKEKKELISEKIEKYYNSFELDEDYAKKLFENLLEKAIKENA
                     SDIHIEPFKEHLIVRMRVDGELKEVSKFLVDVYPSLSTVIKLKASMDITEKRLPQDGR
                     VDIKLGGNLIDIRVSSIPTVYGEKIVLRILNRDTFLKDKLELGFSEEAIKSINRIINK
                     RAGILLVTGPTGSGKTTTVYSLLNDLKGINKNIMTIENPVEYKMDGISQIQVNSKVGL
                     SFDVGLRAILRQDPDIIMVGEIRDAETAKIAVRAAITGHLVISTLHTNDAVSSIARLL
                     EMQIPPYLLNASLIGVISQKLVRKVCNHCSHEIMIKDNFSGDVNTKVAVGCEKCNDKG
                     YFGRTAIYEILEINDDIKTCIRNMEDSSTIKDVAQNNGMITFEDSCKRLINEKITTLE
                     ECLVVNEMI"
     gene            28838..29923
                     /locus_tag="PC414_001240"
     CDS             28838..29923
                     /locus_tag="PC414_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HDF2463645.1"
                     /translation="MRLSKNIFYNLEKFYLVLNRISDKEIRVICKEIGILLESGCEIT
                     KIFEIIESQSSKKVKNLLSIVSNHIQKGNSIAESFQITGIFSKFFISMIKAGETSGNL
                     DIIMSDLSNYYDKEYKLKMKIITISIYPIILIILSILSMLFIFVFVIPNFQVVFTNNG
                     IEPPLITRVLMGISTVVTNNLAYIIFSFILFSIGSIYFFITNDNIKKLINSLKFKIPF
                     IKKINQLVATTRFSRALSVLISSGIQIVEAIDMSASVIDNEFIYERLKISNNYIKKGN
                     SIGKSLNLANIFPKLFISMANTGEESGKLDKSLNVINKFYENELNNKIEQIMKFIEPM
                     LIVFMGLIIGTFILAMVVPMFDAITNF"
     gene            29977..30336
                     /locus_tag="PC414_001241"
     CDS             29977..30336
                     /locus_tag="PC414_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HDF2463646.1"
                     /translation="MINLINKKRKGFTLVEMIVVVTILGVISSIALVKYSKVQESAKL
                     NADYTNAANIVTAASMAINDDEKTIDSLSVETLKEKGYLNTVPVPQSTSGKFELVIND
                     SGTDISVNINSKQFYPK"
     gene            30389..31261
                     /locus_tag="PC414_001242"
     CDS             30389..31261
                     /locus_tag="PC414_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HDF2463647.1"
                     /translation="MAIIKKILNLEINDDYIRLVFLRKSHKKVFIDKSIIKEINFDIK
                     NDSNSIEKIKYIEDVVRFVKEEIGKSKILYKNLYFNIQIQDIVIRNVEVLNTKKKRDI
                     LSMIEFEITQYIPININNYSIKYKVINSKNDKLNIQVILFPKNIIQLIKNISENLNMN
                     PKTININFDILQKLINLNLIENFSEEGVFIECKKSQLIINITKDKKIRETYLLSKEGK
                     SYEFIKRLLRGFKYVYYYGIENCFIEEYFKYTDKSIEVNPLRLKDKVKVENNFDADLE
                     DNRINYINSIGMII"
     gene            31262..31705
                     /locus_tag="PC414_001243"
     CDS             31262..31705
                     /locus_tag="PC414_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463648.1"
                     /translation="MKVDKTINFLNGFKKTKPCNKIYKIIIVLILTCILFKSFMYVKS
                     INELEDYISQNKLVDKSKNTLPTTDKTREKFSKIKLNEIKKIYETIGINSITKISIDK
                     NKLEMEGVCSDLDVLNNLRKIKYIKYYYIDSIVRDGEYYRFRIKS"
     gene            31720..32304
                     /locus_tag="PC414_001244"
     CDS             31720..32304
                     /locus_tag="PC414_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463649.1"
                     /translation="MRLNNREKNLLKILLILVIILASDIAVSYLEENLTSLKNEKQNI
                     INKINNQDNYQIKDNKHIDDKYIESVELNKNEELKNNFNNSFLDKKTNKDIVLLVENE
                     LRDIVNVEYINQESSLDNHNKKQVNLEIKVNGELANVLKLEGVMKKLRLNNKLEKIEI
                     NKLKVNNFDVQNIPNEYKQEKDNIVECKMTVRVI"
     gene            32557..33594
                     /locus_tag="PC414_001245"
     CDS             32557..33594
                     /locus_tag="PC414_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HDF2463650.1"
                     /translation="MLGRNELCPCGSGKKYKKCCLNKDIVSERACRKTELSQKQYTDL
                     YSKLYDYSRQDKFKEECEKAKEMFYIMQNDSVNEKFERFFNTYLIQDHIMENKKVMTV
                     GFLEENGANLSQSEVSILKSMFESYVSIYEVKEVSAGKIVLKDCLNGNELCTEDVKLL
                     RSFKIGSCMIARIVDIEGTNILIDITISISNEVKDIILKDIMNLFNQYQDVYKDMKSF
                     LIYHTHILYKYIQQLLDPSIAEYLKRERDKNDTKVNSENELAEEECKVLDTLKENVEK
                     ENIDACIEFWNEYKKNHENIKGAENGWAAAVEYYVKKEAGQPITQVQISKKYDISSST
                     LGKRYKDLKIS"
     gene            33675..34040
                     /locus_tag="PC414_001246"
     CDS             33675..34040
                     /locus_tag="PC414_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463651.1"
                     /translation="MSLSLGNAIAVSTNFIKSNPAVPVYNGEEIYAVIYHIEIGYNNG
                     VSPDTKQYIIIDYKEFDLSMDKKNELINIAKEYCKSKGIVENENLEINLLDNEEAENF
                     LEKVFNNMKVIRGLITKKG"
     gene            34045..34362
                     /locus_tag="PC414_001247"
     CDS             34045..34362
                     /locus_tag="PC414_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463652.1"
                     /translation="MDYIGIENITPYENTYEFSVYEYDDEITLGSEKLYVCELRVVLI
                     KVNSLYVERLHKSVEAMVLVKNLKKDLDKTLVVNKIKNFVLDEIWVENLVKENIEVIF
                     VES"
     gene            34410..36014
                     /locus_tag="PC414_001248"
     CDS             34410..36014
                     /locus_tag="PC414_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463653.1"
                     /translation="MLRVSNVKLGIDKDISLLKDVILKKLKIKEKNLVKYSIYKESID
                     ARKKGKMEFVYSVDVEVKDENKILNSKIKDVTKIKEIRYVNVPVGDKKLKNNPLVIGS
                     GPAGLFAGLLLAQMGYEPIILERGLDVDSRTKDISDFWTTRKLKNNSNVQFGEGGAGT
                     FSDGKLTTRIKDIRCRKVLEELVQFGAPDEILYSHKPHVGTDILKNVVKNIRNEIISL
                     GGQVRFNSKVTDIVVSNDSIQSVIINDTEKIDTDTIILAVGHSARDTYEMLYSRGIKI
                     VQKPFAIGARVEHPQDLINKAQYKDFYNHPRLGAADYRLIEHVSNGRTAYTFCMCPGG
                     SVIASASNDFEIVTNGMSEHARDKINANSAFLVNVTPEDFKSDNPLAGVHFQQKYEKL
                     AFELGGKNYNAPVQLVGDFINNKTSLNLGSVEPSYRPGYTLTNLSECLPSFVTETMRE
                     AFISLDKKLNGFAMYDAVLTGVETRSSSPIRIVRDENTLESVSIKNLYPCGEGAGYAG
                     GIVTAAVDGIKCAEKIIQKYTNNTKI"
     gene            36138..37766
                     /locus_tag="PC414_001249"
     CDS             36138..37766
                     /locus_tag="PC414_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HDF2463654.1"
                     /translation="MNIIISLMGGLGLFLYGMNLMGEGLQKSAGTKLKKIIKLLTSNL
                     FMGVLVGTGVTAVIQSSSATTVMVVGFVNAGIMTLKQAIGVIMGANIGTTVTAQLVSF
                     DLTGMAPVALGVGIILYLFGNKPRIKNIAEILIGFGILFTGMDFMKMAVEPLRDYQGF
                     TDLLVTFGRYPLLGLLLGFGITAIIQSSSASMGMLVALAAEGLVPLSAALPILYGQNI
                     GTCVTSLLSSIGANKNARRAAMMHLIFNVLGTVIFLIFLNKPVVSMVTSWDPSNVARQ
                     IANTHTLFNIISVLILLPFTNLIIKLAIKLVPDRAGDIDEDETKTIKYIDDRMIETPS
                     IALANTIKEALRMGEKAKESLNASMEALVEHSTEKIDKTYRRERLINDLQKAILNYLL
                     KLSKAPLDDDSREVVDTLFNTVNDIERIGDHAKNIAELSQVAIDSNISFSEEGQSELD
                     VMYNRVVSAYTYALESMRTDNVDLACKVIKIEEQVDIMEKSCRANHMYRLNNNLCSIE
                     NGVIYLDVISNLERISDHAVNIAQQVIAKRLGND"
     gene            38031..39380
                     /locus_tag="PC414_001250"
     CDS             38031..39380
                     /locus_tag="PC414_001250"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDF2463655.1"
                     /translation="MGKINFDYSKATEFFCQNEIDVMQPYVDVAHDMLHNKTGLGNTF
                     LGWIDLPKNYDKEEFDRIKKSADKIKSDSDVLLVIGIGGSYLGSRAAIDMVSHSFRNG
                     LKKEQRKAPEVYFVGHNISSTYIMDLLDIIEGKDISVNVISKSGTTTEPALAFRIFKD
                     YLEKKYGKEEARKRIYATTDASKGALRQLATEEGYETFVIPDDVGGRFSVLTAVGLLP
                     IAAAGLDIDAMMKGANDAREAFQNPDLKSNDSYRYAVARTILHRKGKDVELLVNYEPQ
                     LHYVSEWWKQLYGESEGKENKGLFPASVDFSTDLHSMGQYIQDGKRLLFETVLNVENC
                     KRNITISSEEVDLDGLNYLAGKTVDFVNHKAFEGTLLAHTDGKVPNLVINIPQLDEYN
                     FGYLVYFFEKACGISGYLLGVNPFDQPGVEAYKKNMFALLGKPGYEKEKEELEKRLK"
     gene            39536..40615
                     /locus_tag="PC414_001251"
     CDS             39536..40615
                     /locus_tag="PC414_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDF2463656.1"
                     /translation="MSRRKKGISLVILVAIISSILSSFLTIILVKDNLVSKSTGSSTP
                     IVVNDDGKSQNIYQAVAEKATPSVVGITTTSVDTSNMFAIPTETQGVGTGIIVDSNGY
                     ILTNSHVISDGQATSVNVLFNDGSTTSGKVVWFDQQLDLAIVKVDKTGLTPAEFADSD
                     KVKVGDISIAIGNPLGLDFQKTVTQGIISGLDRTIQTEKTNMTGLLQTDASINAGNSG
                     GPLLNQKGQVIGINTAKASQAEGLGFAIPINTAKSIVEEVIKNGKYEKVTLGIKGTDV
                     SNYEAATGTKLSTDKGVYVAEVISGSSAEKAGVKVGDIITKVGDTDITGMNDLNKKLY
                     TFSKGASTKITVNRGGKAVTINVNF"
     gene            40960..41868
                     /locus_tag="PC414_001252"
     CDS             40960..41868
                     /locus_tag="PC414_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HDF2463657.1"
                     /translation="MNPLVINLQKCSIHDGPGIRSTVFFKGCPLECVWCHNPESQTYT
                     KQVLYNEERCSKCEACINICPHKAIYKGETKICLDQDKCEFCETCLDYCVNNAREIVG
                     QEYSVRDLVKEIEKDRIFYEESGGGVTLSGGEVMAQDMDFICGVINMCKSKGIHVAID
                     TCGYAKSENYERVAKCADLFLYDIKLIDEDKHIKFTGKSNDLILKNVKILSELGANIN
                     IRIPLIVGVNVDDENLEVKKMIEFLKPLNIQAVSLLPYHNIGKHKYDKIYKKYEGEEL
                     QRPSEEKLEEIKRLFEASNFNTKIGG"
     gene            41873..44242
                     /locus_tag="PC414_001253"
     CDS             41873..44242
                     /locus_tag="PC414_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HDF2463658.1"
                     /translation="MARGTFERTKKLREESINAEPHISIERAVLMTEAYKKYEGSVEI
                     PVLRALSFKHYIENRTLSINDGELIVGEKGDSPNGAPTYPEICCHTMEDLEVMHNRDI
                     INFSVSEEARKIHKEEIIPFWKKRQTRDKIINAMTPEWLAAYEAGMFTEFMEQRAPGH
                     TVCGDTIYKKGFLDLKKDIEARLKELDFLNDLDAYNKKADLEAMAIACDAMVILGKRY
                     AEKARQMAEEETDEAKKKDLLLIAETCDVVPAHKPETYHQAIQMYWFVHIGVTTELNI
                     WDAFTPGRLDQHLNPFYERDVENGILDRDRAQELLECLWVKFNNQPAPPKVGITLKES
                     STYTDFANINTGGINPDGQDGVNEVSYIILDVMDEMKLIQPSSNVQISKKTPQKFLKR
                     ACEISRKGWGQPAFYNTEAIVQELMEAGKTIEDARLGGTSGCVETGCFGKEAYVLTGY
                     MNIPKILELTLNNGYDPISKKQIGIETGDPRNFQSYEELFEAFKKQLHYMIDIKIEGN
                     AVIENICAKHMPCPLMSTIVDDCIEKGKDYQRGGARYNTRYIQGVGIGTITDSLTAIK
                     YNVFDKKKFDMDTLLKALDANFEGYEAILNLVSNKTPKYGNDDDYADEIMQEIFNAYY
                     NEVTGRPTVCGGEYRVDMLPTTCHIYFGEIMGASPNGRLCAKPVSEGISPEKGGDTNG
                     PTAVIKSCAKMDHIKTGGTLLNQRFAPSVVQGEKGLDNMANLVRAYFNMDGHHIQFNV
                     FDKNVLLEAQKNPQDYKDLIVRVAGYSDHFNNLSRTLQDEIIGRTEQTF"
     gene            44567..45370
                     /gene="proC"
                     /locus_tag="PC414_001254"
     CDS             44567..45370
                     /gene="proC"
                     /locus_tag="PC414_001254"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDF2463659.1"
                     /translation="MKTLGFIGSGNMGSAMIGGIVQSGLVKAEDITVSDLSQVALDKV
                     KEKFGVNTTTDSKVAVTNSDVVIVALKPNICEKALTPLKELIDANKIIVSIAAGKTIE
                     SLESFIGSDKKIVRVMPNTPALVGEGMSALCKNSNVTDEELNMIKALFESFGEAEIVS
                     EYLMDTVTGISGSSPAYVFMFIEAMADAAVLAGMPRQQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPAGTTIEAVKVLEEEGFRAAVIKSIVACIDKSKDMSK"
BASE COUNT        18674 a         4819 c         8239 g        13759 t
ORIGIN      
        1 aatatgagag tattatggtt aatgtgaatt ttaaagatat tattaaatat ataaattaga
       61 atactattaa acggataaat ggaggagtta ttatgtctaa gatattagta gtagatgatg
      121 agttagatat gttaaagttg attgaaaatg tgttgaaaag agatggacat gaagtaaagg
      181 taattagtga tgtagaaaaa gctatgaaca tggattttaa ttattttaat ctaataatat
      241 tagatgttat gatgcctaag atggatggtt ttgaagtatg taaaactata agaaataagg
      301 ttgattgtcc aatactgttt ttgacagcaa aaaatatgga aagtgatatt atgtacggtc
      361 ttggtatagg tgcagatgac tatataacca aaccttttgg tgtaggagaa ttaagggcta
      421 ggataactgc tcatttaagg cgagaaagta gagaaaaaaa gaatttatta actatctcaa
      481 atgttcaatt taacttttta ggcaaagaag tttcaattga tggtaaaaaa attaatttta
      541 ctaaaagtga atatctaata tgtgaatttc tagcaaaaaa taagggacaa gtattttcaa
      601 aagaaagaat atatgagtcg gtatatggtt ttgatggaga aagtgatata agtactatca
      661 cagagcatat aaaaaatata cgaagcaaat taaaacaata tgatatttat acaatagaaa
      721 ctgtatgggg gatagggtat aaatgggtgt aataagtata agtgttgttt agtgaaaata
      781 ttatatatat ctacaaaatg tttgtaaaat attaatctat atattaaaat ttaatttata
      841 gattaatatt ttttgtaaat gacaaaattg taaggtattt tgttataaaa tattaagatt
      901 catagtgtat actaataata tagtatttag tataaatctt gagcaaagga tggttatttc
      961 taatggatag actaaagaat attagaaaga gtttactaca gtatacattt ttatttttgt
     1021 taataggtat aacaatttac ctagtgttta aaacattgga tattaaaatg ctatcaaatg
     1081 ttatggttat ggtagataag aagtttttat tcataggtgc tttggcaata atgtgtcaca
     1141 taatgcttga aggagtagta atgaagataa ttatagaaag cactcacaaa gttaatatta
     1201 gatttattgg ttttaagcta gccattatgg gattttatta taatttaata actccttttg
     1261 cttcaggaag ccaaccagta cagatatatg tacttaaaaa atgtaaaatg ccacttagta
     1321 aggcaagtgc agttgttacc aataagtcta ttatcttcca aatagtagtt actttttatt
     1381 gtacaatact tgttttaatg aattttgcta tgttagaaaa acaaatgaaa atgattatgc
     1441 ctctagtgtt tctagggata gccattaatt catttgtctt aattatgata atattagtta
     1501 ttttaaatcc tgaaaaaata aaatatttta tgagaattac tataaagcat ttatcaaaaa
     1561 ttaagttttt aaaattttta gataataaga ttgaatccat agaaagtttt atagatgatt
     1621 atagtaaagc aataaatttt tttgcaaaga ataaaaaggt attaatttca actataatag
     1681 taacttttat tcaattaagt gcttacttta gtgtttcatt ttggatatat aaagcattta
     1741 atttacaagg ttatagctat atttatatac tgacattaca agcattttta tatatggcaa
     1801 tatctccaat accaacacct gggaatgtag gagctaatga attagccttt ttcactatat
     1861 tcaaatcagt attccctcaa cctttgatgg gatatgcagt atttttatat ggtggattta
     1921 tgtactattt gattctgatt ggaagtggaa tatttactgt aattactcac tatagaatga
     1981 aaaacagaat tggcagaaat attgttttaa gtgaaaacta aataagttta aagaatatat
     2041 attgagtata ttaaaaaata tgtcataaga actagaatat tttaattaaa tggttgaaat
     2101 ataaagatta ttatgacata ttttttttga aatttaagag gaattgtgag aaaattgttg
     2161 aatttaatag atagtattgc aaaatattta tgattttgca tattataaaa acactcatta
     2221 tctttaagat aaattttact tatcagggga taaatctaag gggggaattg tatggagaaa
     2281 aaaattttaa cagtttgtcc atactgtgga gctggatgcc aattatatct agtagtaaaa
     2341 gataataaga tattaagagc tgaaccggct aatggtagaa caaatcaaga aagtttgtgt
     2401 ttaaaaggtc gttatggatg ggattttcta aatgacccta aaattctaac acctaggcta
     2461 aaaaagccaa tgataagaaa acaaggtgta ctagaagagg ttgaatggga tgaggctata
     2521 aactatacag cttcaagact aaatgaaatt aaagaaaaat atggacctga ttctattatg
     2581 ggaacaggtt ctgcaagagg acctggtaat gaagcaaact atataatgca aaaatttatg
     2641 agagctacaa taggaactaa taatgttgac cattgtgctc gtgtctgaca tgcaccatct
     2701 gtagccggtc tggcttactc attaggtagt ggtgcaatgt ctaattctat accagagata
     2761 gaaaatgcag atgttttatt tatatttgga tataatggag cagattcaca tccaatagtg
     2821 gcaaatagaa tagtaaaagc taaaaaaaat ggtgctaaat taattgttac tgaccctaga
     2881 gtaactgaat ctgcaagaat tgctgatata catctaccta taaaaggtgg aacaaacatg
     2941 gttttagtaa atgcttttgg aaatgtatta atagaagaag gtctttacaa taaagaattt
     3001 gttcaaaatc atacacaagg atttgatgag tataaagaaa ttgttaaacc atatacagct
     3061 aaatatgctg aaaagattac aggaatccca gaagagctta taagaaaagc tatgagagaa
     3121 tatgcaaaag gtaaaaaagc tatgatactt tatggaatgg gtgtctgtca atttggtcaa
     3181 gctgttgatg tagttaaggg acttgcatca attgctctac ttacaggtaa ttttggtaga
     3241 gaaagcgttg gaataggacc tgtacgtgga caaaataatg ttcaaggagc ttgtgatatg
     3301 ggagctcttc ctaatgtata tcctggctat caaaatgtaa ctgatgataa gataagagaa
     3361 aagtttgaaa aagcttgggg ggtaaaatta tctcctaata atggttatag tttaactcaa
     3421 gttccaaatt tagtactaaa agagaaaaag cttaaagctt attatatatt tggagaagac
     3481 ccagtacaaa gtgacccaga tgcatcagaa gttagagaag cattagatga attggaattt
     3541 gtcattgttc aagatatatt tatgaataag acagcacttc atgctgatgt tatacttcca
     3601 gctacttctt ggggagaaca tgagggagta tatacatgtg cagatagagg attccaattg
     3661 atgagaaaag ctatagagcc acaaggagat gtaaaaccag actggcaaat tataagcgaa
     3721 atttctacag caatgggata tcctatgaat tataaaaata ctaaggaaat atgggacgaa
     3781 cttagacaat tatgtccaag tttcttaggt gctacttatg aaaagataga aactcaagga
     3841 tgcgttcaat ggccttgtaa gagtgaaagt atggaagata aaggaactat gtatctgtat
     3901 gaaggtcaaa agttttctac tcctaatggt aaaggtaatt tgttcgctgc tgaatggaga
     3961 ccccctatgg aagttgaaga tgatgaatac ccatttagtt tatgtactgt aagagaagtt
     4021 ggacactatt cagtaagaac aatgactggt aattgtagaa cacttagttc attagaagat
     4081 gaaccaggac gtgttcaaat aaattctaat gatgctgaaa aattaggaat tgaagatgat
     4141 gaattagtta gaattagttc aagaagagga agtgtaataa caagagcaac agttactgat
     4201 agagttaagg aaggtgcaac atatatgact tatcagtggt gggttggagc ttgcaatgaa
     4261 cttacaatag caaatttaga ccctatatct aaaacaccag agtataaata ttgtgctgtt
     4321 aaacttgaaa aacttgaaga ccaagaatta gcagaaaaat gtgtaagaga agagtatcaa
     4381 agtttgaaag acaaaatgac agctacaaat atctaatgat ttattagatt taagatttat
     4441 aaaaggcaga aaggctaata ttttatgaac aatatatact caaaaactaa attacaagaa
     4501 aatagtaagg ataaagtttt tgaaaatagt tatagtctag tggactacaa atatttaaat
     4561 tcttttaatg tagaagtaag caaaataaat gaacatgaag tatcaataga taatgaagaa
     4621 ataattgcag aatatccact tagttttatt ttaaatgata aatatgtaaa tacattttta
     4681 tgtaccccat ataatttaga ggaacttata gtaggattct tgaaaacaaa aggatacata
     4741 gaaaacaaaa aaagcttatt aaaatttgag ataaatgaga gtgaacgttt tgcaaaagta
     4801 actattcaag agaaacaaaa aaatcaagat gagcaaatta tatttttaaa ttcactagac
     4861 tatataacat gtgctcctgt aaagagcaat ataaaaataa attatgatac gatttacagt
     4921 ataatggata aaaatttaaa ttcatcagaa ttatttaaaa atactggtgg ggtacatagc
     4981 gtatctgtat ttaattttga taaaaaaata gtcacttgtg aagatgtagc aaggcataat
     5041 gcaatggata aggctattgg attttgtgtt ttaaatgaca taacattaaa agataaaatt
     5101 gtggctgtaa gtggaagaat atcattggaa atgatattaa aagcagctat gactcaaatt
     5161 cctattgtag tatcaaaatc agcaccgact aatttatcaa tagagctttc taaaaaatta
     5221 aatataacgc ttattggatt tgtaagagga agacggatga atatttatgc aaatccacag
     5281 agagttatca aaaagtgaga atgaatatat gtgtactaaa aaagaagagc aaagatattc
     5341 aaagtgtgaa atctgtggag tattaattga tgatactgat aaggtgtata ataacacata
     5401 caatttgtgc aaaaaatgta aacgaaatat aagtactgag aaaatagtga aatatataaa
     5461 tatatatgat gaaagtaagt agacatatga tgaaaataac tagagggttg gaaatataca
     5521 aattgaaagg tgggataata tgaaaaataa aataggctct tttgtaatag cagacccaaa
     5581 caaatgtaca ggttgtagag cttgtgaagt agcttgtttt acaatgcata atcagcataa
     5641 taatgtgggt tatacagttg gaacagttga tatacctgtt atacctagac tttacttagt
     5701 aaaaggtgac aatttctgta tgccaataca gtgtagacat tgtgaagatg ctccttgttt
     5761 aaactcatgt ccacaaaaag ctatagttaa agaaaacaat attatgtctg taaatgaaga
     5821 aaagtgtata ggatgtaaaa cttgtctgct agcatgtcct tttggagcta tagacttact
     5881 tccacaatat gaagatggta aagaagtaga acaagtaatt ttagatgaaa ataagaagat
     5941 agcttataaa tgtgacttat gtaaagacaa tgaaaaaata gcttgtataa gtgcatgtcc
     6001 tcaggaagca ttaaaacttg ttactccaat agatgacaaa aaagctaaga atagacaagc
     6061 tgcacttagt ttattgttaa caaataaata aaggatacag gggtaggtga cgtttatgat
     6121 aataaatata gataaggatt tatgtactgg atgtagagaa tgttcaaagg tctgtccagt
     6181 aaatgctatt gaaggtgaag agggaaagcc acaagagata aatttagata gatgtgttat
     6241 gtgcggtcaa tgtgttcaaa cttgtaaatc ttatgcctca gttatagatg aaggatttga
     6301 gtttttacaa gagaaaaaac aagaaagaga gataccagag tctataaatg aacctatatt
     6361 tgcagcatac aatgtatgtg atatagataa ggtgaagaaa gctttaagtg acccaaatgt
     6421 attcactatg gtccaatgtg caccagcagt tagagttgct ttaggagaag attttggata
     6481 ttatttagga tacttaggtg ctgggaaaat ggcagcagca cttagagctt taaattttga
     6541 tagagtctac gatactaatt ttgcagcaga tttaactata atggaagaag gtagtgaatt
     6601 aattaaaaga gtgacagaag gtggaactct tccaatgttt acatcttgtt gtccagcttg
     6661 ggtaaaattc atggaaaaaa attatcctaa attaactaat catttatctt cttgtaaatc
     6721 acctcagcag atgggtggtg caatatttaa aacttatggt gcagagataa ataatgtaga
     6781 tgcatcaaag atatttagtg tttctataat gccatgtaca tgtaaaaaat atgaatgtga
     6841 tagagaagaa atggattcta gtggatatag agatgtagat gtagtattaa ctactagaga
     6901 actagcttat ttaataaaag acatgggaat tgattttaaa aatttaagag aagaaaaatt
     6961 tgatagtcct ttaggtagtt atagtggagc tggaactatc tttagtgtta ctggtggggt
     7021 tatggaagct gctataagaa caggatatga gcttataact ggagaaagta ttcctgatgt
     7081 agaagtaaaa caagtaagag gagaaaatgg ttttagaaag tcaactatta aaataggaga
     7141 cttagactta agagttggag tagtaagtgg attaaaaaat gtaataccag ttcttgaaga
     7201 cttggaaaaa ggaaaattag atgtagattt tattgaagtt atgacttgtc ctgttgggtg
     7261 tgtaagtggt ggaggtcaac caaaaatttt actagaagaa tataaggaat tggcttatga
     7321 aaatagaaca aaagcaacat acgtgcatga tgaacattta gcaattagaa aatctcatga
     7381 gaatcctgat attaaaaaac tgtatgatga atatctggta gaaccattag gagaaaaatc
     7441 tcatcatcta ttacacacta catattgtat aggaaaggaa gtggctaagt gatgaactat
     7501 tttgttatag cagaccctga taaatgcata ggatgtagaa cttgtatgat aggatgtgta
     7561 gttgcacatt cagatgaaga tattttttac caaaatccag atgaaataaa ttttaatcca
     7621 aaactttctg tcataaagac aaaagaagta agtgctccaa tacagtgtag acattgtgaa
     7681 gatgctcctt gtgcaaaggc atgtccaaat ggtggtatag ttagggttgg taacacaata
     7741 aaaataaatg aagaaaattg tataggatgt aagacttgta tgttagcatg tcctataggt
     7801 gcaatagaca tagttacatt aaaagatgta gatgaaggaa agctttgctt tagagaaaga
     7861 atgacagcaa ataagtgtga tttttgtata gaatcgccag aagggcctgc atgtgtaaat
     7921 gtttgtccaa ctaaggcatt tactatagtt aaggatgagg atatagatag taaagttaaa
     7981 aataagagaa aattagctat attgtaatat aaaaaagatg gagttttatt tatgattaaa
     8041 agctccatct tttttattat aatatttata tatctaaaaa tcaattattg cattagttta
     8101 tctaatcttt ctttattcaa ttttttcatt cctatagaag ttataataag taaagtatag
     8161 gcaattataa ctgataaaat tataatagac caagaaaatc tgtatccaaa attatcaact
     8221 aacaatccaa ataatgttga acctaatgca aatcccactg caaaaattaa ttgtacgatt
     8281 ccatatatgc taccaaactc cttttgacca aagaaagagc cagttaaata agcaggacca
     8341 atcatataag caaatacaga aagacctttc aaaatggcaa atacaaatgc aaaagtagaa
     8401 gatttatcta ccaatagtaa tgataaagtt gctataagaa ccaatacaaa agatataata
     8461 agtggctttg tttgaccgaa tttatcaaac aataatcctc caaataaatt accaatgaga
     8521 gcaaataagg caaatgtgga gcctactgtt cctattagaa taggatttag ataatttcta
     8581 agataagttg gatattgtac agataatgat gaaacatata taccaacaaa tataaatcca
     8641 atagtcataa tccaaaaata tttaattttt ttagcttctt ttaatgtata accccatact
     8701 gaaggagaaa ttgtatcaga tgctttctct tttttacctt tagattttac cttttcatta
     8761 atatctttag gcatcctgag tatgaataat attacaggta taccaactat taatgctata
     8821 attccaaata ttagataaga acgactgtat ccttgagttg ctaataagcg aactgcaagt
     8881 ggttgtagaa atatactacc aatagaacca ccagcaaaag caaagcccaa agcttttcct
     8941 ttgctttctt catcaaacca agcacttatt aaaacaggta ttcctatagc agatataatt
     9001 gtagcgccaa tttgaacaat acatgataca gtgtaaaatt gccatagttc agtacatata
     9061 gaaaaagtag caaaagcaat actagaaatt atacaaccta ataaatatag tatcttaata
     9121 ttgactttag aaaataatac acctataaaa ggtgatgcaa ttgctgaggc aatagtacca
     9181 atagtgaata ttagagaaaa agaagctaaa ctaaagcctt ctccttctac aacataactt
     9241 ataaattgag gctgtatact tgctacaata ctaaatggaa cagcttgtat taacatacaa
     9301 ccaagtacta taacccaagc atttgaaaag tttttaatgc taaatttatt gtttagtaat
     9361 gtattcatgg tatctctcct ttaggttatg atttgtagat attaatctaa ttattctaat
     9421 tttatatatt agttttggat agcctaacta tttaaatata atttgggtat taatataaaa
     9481 attaactata aaaattgatt attttaaatg tatggggtaa attaaagtat ataaataaat
     9541 gaaaggatgg ataaaatgtc atttgaaaaa ataaataata ataacctaga taaaatacac
     9601 aataggaagt gtattatgct tgtaaacttt aataaaaaag aattatcatt aattaaaaat
     9661 gtaagtaact ttgctggaat aagagaccat atcatcttaa gtgataaaaa tggagagaat
     9721 ataataagag acattttaga tggaaatata tcaaatgaat gtgaaaatgg gattaagagt
     9781 aagtctgtaa tatttaataa cactccaagt acacaagtaa atgcattttt agaagggcta
     9841 aaaaaagtga gaataaatag accttttaca gctatggtaa cagagacatc tattgattgg
     9901 acattgaata atttaataga aaatttagtt caagaacgta atgctatgtc tttaggaaaa
     9961 acaattgaac ataagtaaat ttaagatatc ttatagtaat tttaactaat gtacattgag
    10021 tatcatatta tattttagaa tagaaaattt taaatgtggg tagatggagg agatttgaga
    10081 tgaaagtttt atttacaata aattatggaa aagaaaaatt tgataaaata agagaattag
    10141 gctatgatgt catacattac tctgaagata gtattgaaaa taatgaggat gtaaatacag
    10201 cagatgtact agttacatac aatccatttg agagattaga tatttctaag atgaaaaatt
    10261 tacaatatat acagacaact agtgtaggta tagaccaact tccaaaagaa gaggtattga
    10321 agagaaatat taagatagct aataatagag gcggatatag tgtacctatt ggtgaaagta
    10381 ttgtattgta tattttagaa atatataaaa atagtgcaaa attttttaaa caacaacaaa
    10441 ataaagaatg gaagatggat ttttctgtat cagaattgta tggacaaaga gttggatttt
    10501 taggaacagg tactatatct tcagaagcag caaaaagatt aaaagctttt ggtgtagaaa
    10561 tatggggtgt caatactgat ggaagtagta aaaaatactt tgataagtgt tttgctactg
    10621 aggatatgaa tattgtattt gaagaatgtg atatagtagt tgtagcaatg ccttctacaa
    10681 aaagtacaga tggaatagta aacaaagata tgtttaaact tatgaaagat ggttctgtat
    10741 ttataaatgt aggtagagga aatacagtta atcagaaaga tttagaagaa tgtgtagaga
    10801 aatttagagg ggtagcatta gacgtatttg aaagtgaacc tttaaacaag gacaataagt
    10861 tgtgggagtg cgaaaatgtc atagtaactc ctcataattc ttgggtgtca gataaaaaca
    10921 aagagagaac atttaatatg gtttataata atttgaaaca ctatataatg gagaataaac
    10981 ctgtagataa tgttgtagac atatcaagag gctattaaaa ataaaaaatt ttgataaaaa
    11041 taaaaactct tattagtcgt attaaagata ctaataagag ttttttttgt aataaattta
    11101 aagcccaaaa agtgatatac taaaaaatat ataaatagga gataattaat tatagggaaa
    11161 tttcaaaata atttaatttt aaaaatatga agaggtgcaa aaagtgagcg taaaagatga
    11221 aattaataaa cttatagaga caataaacta ccataatgaa aggtattata atcaagatag
    11281 cccagaaata tcagattatg aatacgataa gttaatgaag gagttaattt ctttagaaga
    11341 agaacatcca gaactaaaga gaattgattc tccaacaaat agggtaggag gtaagccatt
    11401 agataagttc aatcaaattg tacataaaac tcctatgttg agtttatcaa atgctttttc
    11461 agagcaagat ttgagagatt ttgataagag agttcaagaa tatgtgggag aaaatgttga
    11521 atatgttgtt gaatttaaaa tagatggtct ttctgtaggt ctaacttata acaatggaga
    11581 gtttgaaaaa ggagcaacta gaggagatgg agttgtagga gaagatattt ctcaaaactt
    11641 aatgacagta aaaagcatac ctcttaagat aaatgataaa aaagaactaa tagttagagg
    11701 agaagtatat atatcaaaag ataattttag aaaagtaaat gaacaacaag aagaacaaga
    11761 acagccatta tttgctaatc caaggaatct tgcagctggt tcattgagac aattagaccc
    11821 aaaactaaca gcaaagagac cattagatat atttgtattt aatatggaaa atatagaaga
    11881 atacgattta gaaagtcaca gtgaatcact agagtactta gaaaaactag gattttcagt
    11941 aagtcagagt tataaagttt gtaaaagtat agatgaagtt attgagcata ttgaatactg
    12001 gacagataac aggggtaatt tagactacga aatagatggt atggtaataa aagtaaacaa
    12061 tataaaacaa agagacgaaa tgggatatac agccaaaagt cctagatggg caattgcata
    12121 taaattccca gcagaaaaga agaaaactaa aatagtagat ataatagtag aagttgggag
    12181 aactggtaca ataacacctt cagcaattct tgaaccagtc agacttgcag gtaccactgt
    12241 aagtagagct acacttcaca atgaagatta tataagagaa aaagatataa aaataaatga
    12301 tacagtatta gttcaaaaag caggggacat aatacctcaa gttttagaag ttataaaaga
    12361 agaacgtact ggagatgaaa tagattttaa aatgccagaa aagtgtccag tttgttcaga
    12421 gccaacaata agattagaag gagaagctgc tgtaaaatgt atcaatatgt cttgcccagc
    12481 ccaaataaga aggggaatta tacactttgt gtcaagggat gcaatgaata tagatggatt
    12541 gggagaatct ataattacac tgctattaaa tgaaaaaatt attcaagatg tatctgattt
    12601 atattatata aaaaaggaag atgtagtcga cctagagaga atgggcgaaa aatctgctga
    12661 caatttaata aattcaattg aaaaatcaaa agaaaatgat ttatggagac ttataaatgg
    12721 attaggtatt aaatttatag gagttaaagc agctaagata cttgcatata actttaagga
    12781 tttagatgaa gtaatttctg caactagtga gcaactagaa gaattagagg aatttggaag
    12841 tataatggca aacagcgttg tcgagttttt taaggaagac aaaaatatga acgttataaa
    12901 caagttaaaa aatgtaggag taaatacaaa atcattggat aatacaggtg aaaaagtaga
    12961 aaaaatattt gaaggtatga aaatcgtatt gactggtaca cttcctacat taaaaagaaa
    13021 tgatgcaaaa gatatgatag aaaaaagagg tggaaaagct acatctagtg ttagtaaatc
    13081 gacaacattt gttttggcag gtgaagaagc tggttctaaa cttactaaag caaatgattt
    13141 aggaattaaa gttatagatg aagagagatt tttagaatta ttaaagttat catcgaaaga
    13201 tgaagtaaaa gctgtattag agagttaaaa agtcgcttaa tttagtagaa aactactata
    13261 attaatgata gaatagttca gtaagctaat aagattacga aaaatgacaa taattaatat
    13321 ttgacaaaat taaaaattat aaaaataaat ttataaaaaa taaaaaatat gagaaaggat
    13381 ataaaaaatg accagaagtt atgatagaag taatgaacag ataagacctg taaagataac
    13441 tagagattat acaaagtatg cagaaggttc tgttcttata gaaatgggag agacaaaggt
    13501 tatatgcact gcatcagtgg aagaaaaagt tcctcctttt ttaagaaata gtggtactgg
    13561 atggataaat gctgaatatt ctatgctacc aagagcaaca caacaaagaa agataagaga
    13621 tgcatctagg ggaaaaattg atggaagaac acaagaaata cagaggttaa taggaagaac
    13681 aataaggtct gttgtagact tgaataaatt aggagaaaga actatttggg tagattgtga
    13741 tgtgattcag gcagatggtg gaactagaac tgcatctata acaggggcat ttgtagcagt
    13801 tgcagaagca atatataaat tatataaagc aaagacaata aaagagatgc caataacaaa
    13861 ttttgtgtct gcaattagtg ttggtattgt aaatggaata cacatgctag atttatgtta
    13921 tgaagaagat tctaaagcac aggtagatat gaatataata atgactgata agtgtgagtt
    13981 tgtagaagta caggggacag gagaagagaa acctttttct agagcagatt taaatttatt
    14041 gttagagtta ggtgaaaaag ggaataaaga attaataagg cttcaaagag aagctttagg
    14101 tgaaatatca gatgagatat tgggtatgga ttatggtaat gaagtagtga ttgctactaa
    14161 taatgctcat aagctagaag aaataggtga aatcttaaaa gattttgaat ataaagtgta
    14221 ctctttaaaa gatgttgatt tagcaggtat agagatagta gaagatggaa aaacatttga
    14281 gcataatgcg ttgataaaag ctagaacaat agcaaaaaag actaaactta tagcaatatc
    14341 agatgattct ggcttagaag tagatgcttt aggcaaaaaa cctggtgttt attcagctag
    14401 atatgcagga gaacatgcaa cagatgaaga aaatagaaaa aaactgttaa aagctatgca
    14461 aaatgtacca atgagcaaaa gaaatgcaag attcgtctct gcaatagcag tagtttttcc
    14521 tgatggaaaa gaatttgtag taagaggaat ttgtgaagga atgataggat ttgaagaaaa
    14581 aggtaaaaat ggttttggat atgatagctt atttatagta aaaggatatg ataagacttt
    14641 tggtgaaata cctagtgtta taaaaaattc aatcagtcat agagcaaacg cactaaaact
    14701 tatgaaacaa gagtttataa aaagggtagt gaagtaatga aaataggcat agtaagcgac
    14761 acacatatga tgattaaaaa tatggaaaaa acaataccat atttgaaaga atgtgaccta
    14821 ataattcatg caggagataa ctttactgat tcaagatata ttcatagcat gactaatgta
    14881 ggtattattg cagttaaagg taattgtgac tttgatgctg ttgaggaaga agttgtcttt
    14941 gaagtggcaa ataaaaccat attcttatgc catggagata agtatggtgt aaaatatgga
    15001 actaatatgt tagaaaaaaa ggcaactgaa gttgatgctg atatagttat atttggccat
    15061 acccataccc catttagaga aattaaagat ggtgtacttt acataaatcc tggaagcact
    15121 tcacttccaa gaggagtatc gtataaaagt tttgtcatta tggatataga agaagatgac
    15181 ataaaaattg aagaaatacg catataaagg tgtatttatc taaaaaaatg gcaaaaaacg
    15241 ctcaaaaaat taaattttat caaataagta caacgatatt aatggaacac ttgtaaataa
    15301 aaaaacttat gtgatataat taagtggttg aattataaat agaatgaatt ataataccgt
    15361 tatcgggtat tacataaata tcactaggag gattagaagg atgaaagcag aactaattaa
    15421 aaaagagggt aataaagtta ctttaaaaat aacagtagat aacaataaat ttgaagcagc
    15481 agtaaataag gcttataata aaagcagaaa taaatacaat atacctggat ttagaaaagg
    15541 taaagctcca agaattgtta tagagactca atacggaaaa ggtatattct acaatgatgc
    15601 tatagaaata ttattcccag aagtttatcc agaagctatc aaggaattag acatagaccc
    15661 aatagacaat ccagatttag atatagaaga aataagtaaa gacaatgggt tggttatggt
    15721 tcttaatgta gaagttaaac cagagtttga attaggaaac tacaagggta tagaaatagc
    15781 taaggttgag aatactgtaa gtgacgaaaa tgttgatgct aaactacaag aaatggttga
    15841 aaaaaatgcc agattagtaa gtgtagaaga caaagcttta gaagatggag atacagctat
    15901 aatagacttc gaaggttttg aaaatggtgt agcttttgat ggtggtaaag gtgaaaatta
    15961 taacttagtt ataggttcaa acacatttat accaggattt gaagaacaat tagtaggtaa
    16021 aaaagctggt gaagaagtag aagttaatgt tactttccca gaagaatatc actcacaaga
    16081 tttagctgga aaaccagttg tatttaatgt aaaaataaat gatgtaaaag taaaagaatt
    16141 atctgcactt gatgatgaat ttgctaaaga tactagtgaa tttgattctt tagatgaatt
    16201 aaaagcagat gtaagagcta agttagaaga agaagctaaa aatagagcag atgcagaaac
    16261 tagaaattca gtagtagaaa aagttgctga aaatacagaa atagaaatac cagaagttat
    16321 gatacaacat caaatagata atatgttaaa tgaacttaac taccaattac aatatcaagg
    16381 atttggatta caacaattac tagagatgac tggtagaact atggaagaat taagagaaga
    16441 gagaaaagaa gatgctaaaa aattagttaa gtcttcttta gtattagaag caataactaa
    16501 agctgaagga attgaagcaa cagaagaaga atttaaggct gagttagaaa aaatggcttc
    16561 tgcttacaat atggaagtag aaaaaataga agcttcttta agagatgctg acaaagaaga
    16621 tataaaaggt caaataaaaa taagaaaaac aatagattta ttagttgata atgcaacaat
    16681 agcttaataa aataaacgct aactaatata gttgacgtta tgaaatgttt agtatataat
    16741 gatgtgcaaa tataatttag ttaaatgcgt ataataattt acagtagctt aaaaaaagct
    16801 actgtaaatt atatagaata aagggggtat gaaaccatgg cattagtacc tgtagttgtt
    16861 gaacaaacag gaagaggaga aagatcttat gatatttttt ctagactatt aaaagacaga
    16921 ataatcttct taggagatca agttaacgat gccacagcag ggcttatagt agcacagcta
    16981 ttatttttag aagctgaaga cccagacaaa gatatacatt tatatataaa ttctccagga
    17041 ggaagtataa cttctggaat ggctatatat gatactatgc aatacatcaa accagatgta
    17101 agtactatat gtataggtat ggctgcttct atgggagcat tcttgttggc agcaggagca
    17161 aaaggaaaaa gactagcact accaaatagt gaaataatga tacatcaacc attaggtgga
    17221 gctcaaggtc aagcaacaga tatagaaatt catgcaaaga gaattttaaa aataaaagaa
    17281 actttaaatg agatattatc tgaaagaact ggtcagccat tagaaaaaat taaaatggat
    17341 actgaacgtg ataacttcat gagtgcacta gaagccaaag aatatggttt aatagatgaa
    17401 gtatttacaa agagaccgta gatagagaga ggtgttaata tgtctaaata tgaagaaaaa
    17461 cgacaattaa agtgttcatt ctgcggtaaa aatcaagatc aagtaagaag attaattgca
    17521 ggtccaaatg tatatatttg tgacgagtgt gttgaacttt gtgacgaaat tattcaggaa
    17581 gaaatagaag atactataga tgaagatact acatctttac caaaaccaaa ggaaatgatg
    17641 gagattctaa atgattatgt aataggtcaa gaaaaagcta aaaaagcttt atcagttgcg
    17701 gtttataatc attataagag aatatacagt aaaaaatcta gtagtaagga tatagaaatt
    17761 caaaagagta atatactttt attaggacct acaggttctg gaaagacatt acttgctcaa
    17821 actctagcaa gaactctaaa tgtacctttt gcaatggcag atgctacatc attgactgaa
    17881 gctggatatg ttggagaaga cgttgaaaat atacttttaa aacttattca agctgctgat
    17941 tttgatatag aaaaagctga aagaggtata atatatatag atgagataga taagattgca
    18001 agaaaatctg aaaacccatc tataactaga gatgttagtg gtgaaggtgt tcaacaagca
    18061 cttctaaaaa tattagaagg tactgttgca aatgttccac cacaaggagg aagaaaacat
    18121 cctcatcaag aatttttaaa gatagatact actaatgtat tatttatatt aggtggagct
    18181 tttgatggat tagagaagat aattcaaaaa cgtggtggag ataaaacact aggttttgga
    18241 gctaaaatag aaagtaaaaa agaattagat ttagggaagc tgtatgaaaa agtacttcca
    18301 gaggatttat taaaatatgg tataatacca gagtttatag gaagaatacc agttttagct
    18361 actttagagt tgttggatga agatgcatta atgcagatat tacaggaacc taaaaatgct
    18421 ctagtaaaac agtataaaaa gttattggag ttagatgatg ttgagctaga atttgaagaa
    18481 ggtgctcttc gtgcaatcgc taaaaaggcc atagaaagaa atacaggagc tagaggactt
    18541 agaagtatag ttgaaagcgt aatgatggaa actatgtttg aagtaccatc tagagataat
    18601 ataaagaagg ttatagtaac agagaaatct gtgaacgaag actcagtaaa tcctatcata
    18661 gtacttaagg atcaagagga aagtgcataa gatgatgaaa aggagcttta aaagctcctt
    18721 tttttatgtt aatatgctaa atataaaata ggtatcatta ttataaaaat gtgagtaata
    18781 tacctagaat accaaagacg aatacaattg caatggaacc aatttttcct ttatataaaa
    18841 gataaaatga tgctataaat gcaattattc catataaatc ccaattaact tctttggcag
    18901 atgaaaaagc tgctgataat atgaatccta gagtaattgg tctaagaact gtcaataagt
    18961 tttctacctt tttattattt ttaaatttag aaaataactt tgatataata gttagaccta
    19021 ttaatgcaaa aattgtaaca ccaatagttg cagctagaga accaaagaag ccagcagtct
    19081 taaatcctac aaatgtagca ctatttattg ctacaggacc tggtgtagat tgtgatatag
    19141 caagtaaatc caaaaactct tgattagtta taaagtgata tttagatata aattcttgct
    19201 ggataaatgg tagcatggca taaccgccac caaaactaaa tgcccctatt ttaaggaata
    19261 ttaagaagag tagtattatt tctgacatag actatataca cctcctccaa ttattaaata
    19321 aatagggctt atattgaata caccaacaga taaaactgct ataattaata gtagtatgtt
    19381 agttttagac tttggtagtt tcttgaacat gctcgtaaaa gaatacaata taagagctat
    19441 tacaacaggc gcaactcctt taaaaaatcc atctaaaatt ttagatgatg aatttttaaa
    19501 gtatagatac gataagacta agactataca aaatgatggt aaagcagagc ctaaggtaca
    19561 tataagaact cctggtatac cataaagttt atatcctaat aatacagata tattaactgc
    19621 cagtactcca ggataacttt gagctatgga taaataatca ataaattctt cctgctctaa
    19681 aagtttttgt ttgtttacaa gttcatcttg tataagagga accatagcgt atccaccacc
    19741 aaatgtaaac gcaccaattt ttaaaaaggt taaaaacaat ttcaacataa tagaaacctc
    19801 caaaagataa ataacatcta agtatactaa tattaatatt atatcataat aaaaagataa
    19861 tatagtatga tagtattcta agaatttatc attaatgttg aaaattaact tgttcaaata
    19921 tataatatta aaagcaaagc aattagacta gaaaaggagt aatgttgatg gaacaaaatt
    19981 atactaaaat agatcatgaa ttaccattaa ttcctctaag aggattagct atatttccat
    20041 atatgatatt aaattttgat ataggtagag agatatctct taaagcttta gaccaagcta
    20101 tgatggatga agaacttata tttttaactt ctcagaaaga agcagaggta gatgagccag
    20161 gagaagagga tttttatcat gtgggaacaa tctgtaaagt taagcaaatg ataaaattac
    20221 caggagatac agttcgtgtt ttggttgaag gtgtatcaag gggtagagta aagaaaatag
    20281 aacaagaaga tgggtatttc agagcagtaa tagaagaaat agtatttgat agtgataatt
    20341 tggatagtga aacagaagtt gaaattgaag catttgtaag aaatgtattt gatgcctttg
    20401 aagagtatat aaatattgga aatagggtat caccagaaat attaatatct ttagctgata
    20461 tagaagatgt agatagattt atagatacga tagctgcaaa catatatcta aaatcaagcc
    20521 aaaaacaaga aatattagaa gaatttgata ttagaaaaag acttgaactt atttattcta
    20581 tattattaga ggaaatagat atattaaaaa tagaaaagaa aataacttta agagtaaaaa
    20641 aacagatgaa taaggttcaa aaagaatatt atcttagaga acaattaaaa gctatacaga
    20701 aagaattagg tgaagaagaa gatataaact ctgaagcaga tgagtataga gaaaagctta
    20761 aaaaaataaa agcacctaag acaactaaag agaaaataga aaaagagata gataaatttt
    20821 caaaaatatc ttctatgtct ccagatgtat ctgtaagtag aaactattta gatactatat
    20881 tctcattgcc atggaataaa gagacaaaag ataaattaga tataactaag gcaaaagata
    20941 tattagatga agaccattat ggattagaaa aagttaagga gagaatacta gagtatctag
    21001 ctataagaac tcttgctaag tcacttaaag ggcctataat atgtctagtt ggtcctccag
    21061 gaacaggaaa gacatctata gtaaaatcaa tcgccagagc attaaataga aaatttgtaa
    21121 gaatatcttt gggtggagtt agagatgaag cagaaataag aggtcataga agaacttatg
    21181 taggttcaat tccaggaaga ataattaatg gggtaaaaga agcccaaaca aaaaatcctg
    21241 tattcttatt tgatgagata gataagatgg cagcagatta taaaggtgac ccagcttcag
    21301 caatgctaga agtattagac ccagagcaaa ataaggattt tgtggaccat tacttagaaa
    21361 taccttttga tttgtcaaaa atactatttg ttacaacagc aaatagtcta agtaatatac
    21421 caagaccatt attagataga atggaagtaa tagaagtatc tggatatata gaagaagaaa
    21481 aattaaatat agcaaaaaaa tacctacttc caaaacaaat taaagaacat gctttaaaag
    21541 aaaactttat aaaaatagat gatgaaactt taagaagtat aatcaaccat tatacaagag
    21601 aagctggtgt aagaacacta gaaagaacta ttggtaagat ttgtagaaag gttgccaaaa
    21661 aatacgttga agacccaact cttgaagagg ttgttataaa taagtctgac ctagagacat
    21721 atcttggaaa agacatgttt aagtatcaac ttgcagaagt taaccctcaa ataggattag
    21781 taaatggtct tgcatggaca gaagttggtg gagttacact tgaagtagaa gtaaacgtac
    21841 ttaaaggtaa aggagaaatt gtattaactg gaaaattagg agatgttatg aaggaatctg
    21901 cgaagacagg tatctcttat ataaggtcaa tagtggacaa gtttgatata gaccctgaat
    21961 tttataagac gaatgatatt catatacaca ttccagaagg tgctgttcca aaagatggac
    22021 catcagctgg tataactatg gctcttgcag taatctcagc acttactaaa agacctgtgc
    22081 ctggaaatat agcaatgaca ggagagataa ctttaagagg tagagtctta gcagtaggag
    22141 gagttaaaga aaagttatta gcagctcata gagctggtat aacaaaagta ttaattccta
    22201 aagaatgtga agctgattta gatgaaatac cagaaaatgt aaaagaaaaa atggagtttg
    22261 tgcttgtaga acatatggat gaagtattag aacaagcttt attaaagagt ggtgaaaata
    22321 atgaaaatta gaagttcaga gataactatg agtgctgtga ataaatcaca gtatccagca
    22381 gaaggaatcc cagagatagc attggctggt aggtcaaatg ttggaaaatc atcaataata
    22441 aatacattgc ttaacagaag aaacttcgct agaaccagtc aaacaccagg aaaaactaga
    22501 actataaatt tttatctaat aaataatgaa ttttattttg tagaccttcc aggctatgga
    22561 tatgcaaaga tagctaagtc tgaaaaagaa aagtggggag gtataatgga acgatatcta
    22621 gaaagtagac aagagttatg ttctatattc ctacttgtag atattagaca tgagcctaca
    22681 gcagatgata agcttatgta tgaatggata aagcactttg gatataactg tgtagttatt
    22741 gcaactaagg cagataaaat atctagagga caatatcaaa aacacataag tataataaga
    22801 aaaaaattgc aaatggaaag tagtgaaaaa gtaattcctg tttcttcact aaaaaagact
    22861 ggtgttgaag aattatggga agagattgta aatcaataca atcaacatgg atatgaaata
    22921 acagtagatt aaatttaaaa tgttaaatat tgacatgaga tttatttgaa taataaatgt
    22981 aataatagtt agttttaatt atattaatat taaagcaaaa tataggatgc cttaaaatag
    23041 ttttaatgaa ctttttttgg catccttatt gtttataaac aataaatatc agtaattttg
    23101 ttaaatacta ataaatttta gtaaggtatg ggaaaattag tataaattag ttcaactaaa
    23161 aaattattga cttatgtagt cagcaataaa tagcatttaa gaaatagatt tatctgtttt
    23221 tctcataaag aaaacagtta gtataagccc tataaaacat actgatgctg caatccaata
    23281 agactgattg ctagctataa cataggctga tgtgtgactt ttaaactttt gattgtatgt
    23341 aagactttgt gcaaaaaaac tactaaaaaa tgctgttcca agagcaaatg ataaattacg
    23401 aatagttgca agaaaactgc ctccgtatcc ctgtttttga ggaggaacat ctgctaaaat
    23461 actactattg tttggtgaac taaatgctcc tagcccaagt ccaaaaataa ctaaacatac
    23521 aattaaaaga tacaatggag aatctgcttt taaaagtgcc attccaacta gtgagatggt
    23581 cataaatgaa aaagatgctg ttagaatgtt ttttgcaccg tgtttatcag aaagtgaacc
    23641 tgcaataggt gctataagta ccataataag tggatgtaca gtcattacat aaccagccat
    23701 cataggagat aaatttttta attgttctaa ataaaatgga agtagaaaag atactgccat
    23761 ttgaggaaaa taacaagcta ctccaatgat attgcctaca gtaaagtttc tatttttaaa
    23821 tagaggtagg tcaataaagc tttgttcaaa ttttacttct cttaatgcca acaataagaa
    23881 gattataagt gctgttatac ctaaatacaa tcttgtatta cccttctcat tcattgcaag
    23941 taatataagc gtagtgcaag atgcgaaaag tagaagacct ggaatatcaa agctcttgtt
    24001 ttgttcatcc aaaacaggtt taggtagaaa tttaatgcca agaaagactg ctataatacc
    24061 aataggtata ttgataataa aaatagaagg ccatccaaaa tgttctaaaa ttatacctcc
    24121 aattacagaa ccactcatat ttcctatacc aactacagct ccagtgattc ctatagcttt
    24181 tcctttttca tttgctggaa atgcattgga aactatacca agcccagtag ccattaaaat
    24241 acttgctcca actgcctgaa agattcttga agaaattaat gttgataaat tgtttgacat
    24301 actacacaga aaagaaccta ttgtaaatat aaaaaatcct aatgtgtata gacgatttga
    24361 acctacttta tcgccaagtt ttccgaaaaa taacatagta gctgttacaa caagcatata
    24421 agctgttaca acccactgaa tttgtgacat gttaatttta aagtcagcag caattgtagg
    24481 atttgcgata tttagtatac ttccatcaag agtagacata aatacaccaa tacagatgat
    24541 aatcaatact atccattttt gtttcatgat aacactccaa tcttaataga ttttataagt
    24601 acgatataat tgtaaaataa acaacaaaaa aagcacctgt atattaggta ccttgaatgt
    24661 gtctaatttc atgaataaat acggtagttg aaggatttgt atagattact catgtaaaat
    24721 tacataaagg tactcatata ctattttctt ctaccatcca gattttactg tcggctttgg
    24781 aatatgacca aattatgcca taaggctcgc gggcttttac cgccgatagg gaatttcacc
    24841 cgtttatgaa gaaaaacaga tgtattttgt aatattattt taacataaaa ttataatgga
    24901 aacaattgga tttaagtaat atccagctgt attacaatac aaatatttca atatggacac
    24961 ttaaattgta tttatagata gaataaggaa cagtatgaga atctataata attagataaa
    25021 attaacctgt tattagtatt agatggtaag ttttaaattt atcaaaatca gaaagctgta
    25081 ataaaattct tataatatga atatcttaat taagagatat tgtaaattat gaggaggcgc
    25141 taatatggaa ggaaaggtaa agaaggcatt cccgggtgga aatacatcac aaggctcata
    25201 ttcttttttt gactatataa ttcctgaaaa tgtaaaaaga gttttttgtt taaagggtgg
    25261 acctggagta ggaaaatcct cacttatgaa aaaaatttat aaagagtttt tgcatagagg
    25321 atatgatatt gatttatatc attgtccttc tgacccaagt tcgttggatg gtttagttat
    25381 aaagaaatta ggcgtggtac ttatggatgc aactgcccca catactatgg acccaaagct
    25441 acctggagct ttggatgaga taattaattt aggtgattac tggaatacag aagaattaga
    25501 aaagaataaa caagaaatat cagtttatga taaagatatt agtaattcat ttaaaagagc
    25561 atttaagttt ttaaaatcag cagaaccaat ctttagagac atagaagaaa aatattctga
    25621 ttgtatggat tatggaaaag taaatttggt cacagaagaa tttataaaaa ggttatttga
    25681 tggagttaaa agcacaggaa atctaaaaag agaaaagcat ttatttggtt cagctataac
    25741 tcctgtaggg tgtttagatt atacagaaaa cattttaaaa gatgtaaaga aagtctatta
    25801 tttagatgga gaaataggaa ctgggaaaac tacattatta aacaaagtat ataagaaagc
    25861 tattgaaaaa ggtttaaatg tagaagttta tcattatcca cttattccag agaaaattga
    25921 aactgtacta ttaactgatt tagatatagg cataactata agtacgactt ttaaagaaga
    25981 agatacaatt gatttaaatc agtttttaaa tagagaaaaa ctacttaaat atgaagaaga
    26041 tattaaattt gatgaaaaag ttttagatga tttaatttgt tgggcagtac taaatcttaa
    26101 gagagccaag tctaaacatg atattataga aagttactat agtcctaata tgagatttga
    26161 tgaagtaggg aaattaagag ataagttaat taaaagaatt ttaaaatatg aggaaaactt
    26221 ataaacgcat atgtttaaaa ttaacgttag taataatata atttttagta aattggtgta
    26281 gctttgaggc tatgccattt tacttttttt gctaaaaaat gatattaata atctccaaag
    26341 ccattatttg tttttcataa ttattatttc ctttcctaat ttatgttttt ttataatatc
    26401 acaacaatgt ttattgaaag tttatataat tttaagaaca tctaaaaatg catacttgat
    26461 attggtaaat tagtgatagg agttcaatgt atgatattat atactaaaga tgaattttaa
    26521 tatataactt tgatataata agcatatgta aatgtttaat atttaattaa agaaaattat
    26581 catataaaaa taaatttgta aggagcaata cagtatgaaa aaacaatatg tgtttataat
    26641 actagcaagt actattttag gtatacttgt tggaaccata tttagagatt atagcaatga
    26701 agaaacagtt tatacaagca aagacaagtc aataagaaaa gaaataaaaa tgacaagaaa
    26761 atctataaaa gaattaacca aagagaaaga caagttagat aaagaaatta attcaatgaa
    26821 acaaaatcaa aaaaattctg aagaaatatc agaaatagaa acacttagag atagtctatc
    26881 atattcagat gtaaagggta gtgggataat attaagtata gatgctatag atgaaaaaac
    26941 agggaatata gctaatagta tagactataa taagattctt gtaaaagtta taaatgaatt
    27001 aaaagtaaac ggaggaaaat acatagaaat aaatggtcaa agaattaatc aatattcaga
    27061 agttgtactt gcaggaagcc atataaatat aaatggagtt cctgtagcac aaccatatga
    27121 aataaagtgc attggagata aggagaaact atctaattat gtagataaag gtagtaaata
    27181 tttagatagc atacaaacca attatccaat gaaaataagc ataaaaactg aagaaaatat
    27241 ctctatgaaa aaaatgaata tacctaataa attaaagtat ataaagggtg attaagatta
    27301 tattagagga aagcagtttg ccaatgttaa taccagaaaa acttgcaaaa aaatataatg
    27361 tatttccagt tgagtttcaa gatgataatt tagttgtaga aatagaagat gaggatattt
    27421 atgctctaca ggacttgaga ttagctacag gtaaagaaat tatattgaag aaggagaaaa
    27481 aagaacttat aagtgaaaag attgaaaaat attataatag ctttgaactt gatgaggatt
    27541 atgcaaaaaa attatttgaa aatcttttag aaaaagctat aaaagaaaac gcaagtgata
    27601 tacatataga accatttaaa gaacatttaa tagtgagaat gagggtagat ggagaactaa
    27661 aggaagtatc aaagttttta gttgatgtct atccatcatt gtctacggtt ataaaattaa
    27721 aggcatcaat ggatataaca gaaaaaaggc ttcctcagga tggtagggtg gatataaagt
    27781 taggtggaaa tttaattgac attagagttt catcaatacc aactgtctat ggtgaaaaaa
    27841 tagttttgag gatacttaat agagatactt ttttaaaaga caaattagaa cttggttttt
    27901 cagaggaagc tataaaatct ataaatcgta taataaataa aagagcagga atattattag
    27961 tgacaggtcc tacaggtagt ggaaagacta caacagtata ttccttatta aatgatttaa
    28021 aaggaataaa taaaaatatt atgactatag aaaatcctgt agagtataag atggatggca
    28081 taagtcagat tcaagtaaat tcaaaagtag ggttgagctt tgatgtaggt cttagagcga
    28141 tattaagaca agacccagat ataataatgg tcggagaaat aagagatgca gaaacagcta
    28201 agatagcagt aagagcagct ataacaggtc atttagttat aagtacttta catactaatg
    28261 atgcagtatc ttccatagct agattactag aaatgcaaat accaccatat cttttaaatg
    28321 cctctcttat aggtgttata tctcaaaagt tggttagaaa agtctgcaat cattgtagcc
    28381 atgagattat gataaaagat aattttagtg gagatgtaaa tactaaggta gctgtagggt
    28441 gtgaaaaatg taatgataaa ggatactttg gaagaacagc aatttatgaa atcttagaaa
    28501 ttaatgatga tataaagaca tgtataagaa atatggagga ttctagtact attaaagatg
    28561 tagcacaaaa taatggaatg ataacatttg aagatagttg taaaagacta ataaatgaaa
    28621 aaataacgac attagaagag tgtttagtag taaatgaaat gatttaactg aattgtatag
    28681 ttaaacttcg tttggtcagt tgtctttatt taatctattt taattaagat gtttgagtat
    28741 aaattatatt taagtgaaat aaaattaaat gcattaaatt agaaaatatt aagtttattt
    28801 aattgtaatt aatataattt ggaaggtatt atagtacatg agattatcta aaaatatatt
    28861 ttataattta gaaaagtttt atttagtatt aaatagaatc agtgataaag aaattagagt
    28921 aatttgcaaa gagataggca tattgctgga atctggatgt gaaattacaa aaatatttga
    28981 aatcattgag agtcagtcaa gtaaaaaagt aaagaacttg cttagtatag tatcaaatca
    29041 tattcaaaaa ggtaactcaa tagcagaatc atttcaaata acaggtatat tttctaaatt
    29101 ctttataagt atgataaaag caggagaaac tagtggaaac ttagatataa taatgagtga
    29161 tttatctaat tattatgata aagaatacaa attaaaaatg aaaataataa ctatttcaat
    29221 atatccaata atacttataa tattatctat cttatcaatg ttgtttatat tcgtatttgt
    29281 aatacctaat tttcaggtgg tttttaccaa caatggaatt gaaccacctt taataactag
    29341 agttttaatg ggaatatcta cagtggttac aaataactta gcttatataa tatttagttt
    29401 tatattgttt tcaataggaa gcatttattt ctttataaca aatgataata taaaaaaatt
    29461 gattaattcg ttaaaattca aaataccttt tataaaaaaa attaatcagt tagtagctac
    29521 aacaagattt tcaagggcat tgtccgtttt aattagcagt ggaatacaaa ttgtggaagc
    29581 tatagatatg tcagctagtg ttatagacaa cgaatttatt tatgaaaggt taaagatttc
    29641 taataattat ataaaaaaag gaaatagcat aggtaaatct ttgaatttag caaatatttt
    29701 tccaaaatta tttatttcaa tggcaaatac aggagaagaa agtgggaaac tagataaaag
    29761 tctaaatgta ataaataaat tttatgaaaa tgaattaaat aacaaaatag agcaaataat
    29821 gaaatttata gaacctatgc taattgtatt tatgggatta attattggta cttttatttt
    29881 agcaatggtt gttcctatgt ttgatgcaat tactaatttt tagagagaaa aataaatttt
    29941 attagatgaa acttttgaaa aggagagaat atttaattga taaatttgat aaataaaaaa
    30001 cgaaaaggat ttacacttgt tgaaatgatt gtagtagtaa ctattttagg cgttatatct
    30061 agtatagcat tagttaagta tagtaaggtt caagaaagtg ccaaattaaa tgcagactat
    30121 acgaatgctg ctaatatagt aaccgcagct agcatggcaa ttaatgatga tgaaaagaca
    30181 atagactctc taagtgtaga gacattgaag gaaaagggat acctaaatac tgttccagtt
    30241 cctcagagta catcaggtaa attcgaactt gtcataaatg atagcggaac agatataagc
    30301 gtaaatataa attctaagca attttatcca aaataatatt ttatattgat tgctgtaagt
    30361 tttaaaatta tttttattta agaaggagat ggctataatt aaaaaaattc taaacttaga
    30421 aataaatgat gattatataa ggcttgtatt tttaagaaaa agtcataaaa aagtttttat
    30481 agataaatca attataaaag agattaactt tgatataaaa aatgatagta attcgattga
    30541 gaagattaaa tatatagaag atgtagttag atttgtaaaa gaggagattg gtaaatcaaa
    30601 aatattatat aaaaatttat actttaacat tcaaattcag gatatagtca ttagaaatgt
    30661 tgaagttcta aatactaaga aaaaaagaga catattaagc atgattgaat ttgaaataac
    30721 acaatacatt ccaataaaca taaataacta ttcaataaaa tataaagtta taaattctaa
    30781 aaatgataaa ttgaatatac aagtaattct tttcccaaag aatattattc aattaattaa
    30841 gaacatttca gaaaatctaa atatgaatcc aaagactata aatataaatt ttgatatatt
    30901 acaaaagctt ataaacttaa atttaataga gaatttttca gaagaagggg tatttataga
    30961 atgtaaaaaa agtcagttaa ttataaatat aacaaaagat aaaaaaataa gagaaacata
    31021 cttgctatca aaagagggaa aatcatatga gttcataaaa agattattaa ggggttttaa
    31081 gtatgtttat tactatggta tagaaaattg ttttattgaa gaatacttta aatatacaga
    31141 taaatctatt gaagtaaatc cattaagatt aaaagataaa gtaaaagtag aaaataattt
    31201 tgatgcagat ttagaagaca ataggataaa ttatataaat agtataggaa tgattattta
    31261 agtgaaagtg gataaaacaa taaattttct taatggattt aaaaaaacta aaccatgtaa
    31321 taaaatctat aaaataataa tagtcttaat tttaacgtgt attttattta aatcatttat
    31381 gtatgtgaaa tctataaatg aacttgaaga ttatataagt caaaataagc tagtggataa
    31441 atcaaagaac actttaccaa caacagataa aactagagag aaattttcta aaataaaact
    31501 taatgaaata aaaaaaatat atgaaacaat tggaataaat agtattacta aaatatcaat
    31561 agataaaaat aagctagaga tggaaggtgt gtgtagtgat ttagatgttt taaacaattt
    31621 aaggaaaata aagtacataa aatattatta catagacagt attgtaagag atggagaata
    31681 ttatagattt agaataaaaa gttagatggg tgattatttt tgagattaaa taatagagaa
    31741 aaaaatctac ttaaaatatt gttaatatta gttattatat tggcaagtga tatagcagta
    31801 agttatttgg aagaaaacct aactagtttg aagaatgaaa agcaaaatat aataaataaa
    31861 ataaataatc aagataatta tcaaataaag gataataagc atatagatga taaatatata
    31921 gaaagtgttg aattaaataa aaatgaagaa ttgaaaaata attttaataa ctcattttta
    31981 gataaaaaaa caaataaaga tatagtgtta ttagtagaaa atgaactgag agatattgtg
    32041 aatgtagaat atattaatca agaaagtagt ttagataatc acaataaaaa acaagttaat
    32101 ttagaaataa aagttaatgg agaacttgca aatgtgttga aacttgaagg tgtaatgaag
    32161 aagttaagac taaataataa acttgaaaag atagaaatta acaaattaaa agtcaataat
    32221 tttgatgttc aaaatattcc aaatgaatat aaacaagaaa aagacaatat tgttgaatgt
    32281 aaaatgacag ttagagtcat ataacaaaaa agaatagata aagtaaaaaa ctattatcaa
    32341 ttaataaaat tttatagagg aattagtttt acgataattc atttctacaa gagggattgt
    32401 tgatgatttt atcgaaatcc taattaaagt taatatattg taccaaaata taagtgaatt
    32461 taatagaata tttacttata ctatagattg attaataaaa aaatggtata ataacaaatt
    32521 gtactttaat ttaataataa gggagtggaa gtcatattgt tagggagaaa tgagctatgc
    32581 ccttgtggaa gtggcaaaaa atacaagaaa tgttgtttaa ataaagatat tgtttcagaa
    32641 agagcatgca gaaaaactga actttcacaa aaacaatata cagatttata ttcaaagtta
    32701 tatgattatt caagacagga taagtttaaa gaagaatgtg aaaaagctaa agaaatgttt
    32761 tatataatgc aaaatgatag tgtaaatgaa aaatttgaaa gattttttaa tacttattta
    32821 atacaagacc acataatgga aaataaaaag gttatgacag ttggttttct tgaagaaaat
    32881 ggtgcaaacc taagtcaatc agaagtatca atattaaaaa gtatgtttga atcatacgta
    32941 agtatttatg aggtaaaaga agtttctgct ggaaaaatag tattaaagga ctgtttaaat
    33001 ggaaatgaac tttgtacaga agatgtaaaa cttctgagaa gttttaaaat cggaagttgt
    33061 atgatagcta gaatagttga tattgaagga actaacatat tgatagatat aactataagt
    33121 atatcaaatg aggttaaaga tataatttta aaggatatta tgaatctatt caatcaatat
    33181 caagatgtat acaaagatat gaagtcgttc ttaatttatc atacacatat actatacaag
    33241 tatatacaac agcttttaga cccaagtata gctgaatatt taaagagaga aagagataaa
    33301 aacgatacaa aagttaattc agaaaatgaa ttagctgaag aagaatgtaa agttttagat
    33361 actcttaaag aaaatgtaga aaaagaaaat atagatgcat gtatagagtt ttggaatgaa
    33421 tataagaaaa atcatgaaaa tataaaagga gctgaaaatg gttgggcagc tgctgttgaa
    33481 tactatgtta aaaaagaagc aggacaacct ataactcaag ttcaaatatc aaagaaatac
    33541 gatataagct ctagtacact aggaaagaga tacaaggatt taaaaatatc ttaattaatt
    33601 ataaaaagct ttaagtcata gataaagaat ctatagtata atatatgtat aatgtttaga
    33661 aagaggtatt aataatgagt cttagtttag gaaatgcaat tgctgtatca acgaacttta
    33721 taaaatctaa cccagctgta cctgtgtaca acggagaaga aatatatgca gttatttatc
    33781 atatagaaat aggatataat aatggtgtat cacctgatac caaacaatat ataataatag
    33841 actataaaga gtttgattta tctatggata aaaaaaatga actaataaat attgctaaag
    33901 agtactgtaa atctaaaggt attgtagaaa acgaaaacct tgaaataaac cttttagaca
    33961 atgaagaagc agagaacttc ttagaaaagg tgtttaataa tatgaaggtt ataagaggtc
    34021 ttataactaa aaaaggataa ataaatggac tatataggaa tagaaaatat aacaccttat
    34081 gaaaatacat atgaatttag tgtatatgaa tatgatgatg aaatcacctt aggtagtgaa
    34141 aagttatatg tatgtgaatt aagggttgta ttgattaaag ttaattctct gtatgttgaa
    34201 agattgcata aatcagttga agcaatggtc ttagtaaaaa acttgaaaaa agatttagat
    34261 aaaacacttg ttgtaaacaa aataaagaat tttgtgctag atgagatttg ggtagaaaat
    34321 ctagtaaaag agaatataga agttatattt gtagaaagct aggaataacc ctagcttttt
    34381 tatattacaa tctaagagag gaaaacaata tgttaagagt atctaatgta aaacttggaa
    34441 ttgataaaga tatatcttta ttaaaagatg ttatacttaa gaagctaaaa ataaaagaaa
    34501 aaaacttagt caaatatagt atatataaag aatctataga tgctagaaaa aaaggaaaaa
    34561 tggaatttgt ttattctgta gatgtagaag ttaaagatga aaataaaata cttaactcaa
    34621 aaataaaaga tgtaacaaaa ataaaggaaa tcagatacgt aaatgtacct gtgggtgata
    34681 aaaaattaaa gaataatcct ttggtaattg gtagtggacc agctggcttg tttgcaggtc
    34741 tgcttttagc tcaaatgggt tatgaaccaa tcatacttga aagaggtctt gatgtagaca
    34801 gtagaaccaa agatataagt gatttttgga caactagaaa attaaaaaat aattcaaacg
    34861 ttcaatttgg agaaggtgga gcaggaactt tttcggatgg taaacttaca actagaataa
    34921 aagatatacg atgtagaaaa gtattagaag agttagttca gtttggagca ccagatgaaa
    34981 tattatactc acataagcca catgttggaa cagatatatt aaaaaatgta gttaaaaata
    35041 taagaaatga aataataagt ttaggtggtc aagttagatt taattcaaaa gtaacagata
    35101 tagttgtatc aaatgattct atacaatctg taataataaa tgatactgaa aaaatagata
    35161 cagatacaat tatattagct gttggacata gtgcaagaga tacttatgaa atgttgtatt
    35221 caagaggtat aaaaatagtt caaaaacctt ttgcaatagg agctagagtt gaacatccac
    35281 aagatttaat aaataaagca cagtacaagg atttttataa tcatcctagg ttaggagctg
    35341 cagattatag acttatagag catgtttcaa atggaagaac agcatatact ttttgtatgt
    35401 gtccaggagg tagtgtcata gcatctgcat caaatgattt tgaaatagtt acaaatggta
    35461 tgagtgaaca tgcaagagat aaaataaatg caaatagtgc atttttagta aatgtaacgc
    35521 cagaagattt taagagtgat aatccactag caggtgttca tttccagcag aagtatgaaa
    35581 aattagcttt tgaattaggt ggaaaaaatt ataatgcacc agttcagctt gttggagatt
    35641 ttataaacaa taagacttct ttgaatttag gaagtgttga gccatcatat agaccaggat
    35701 atacacttac aaatttaagt gaatgtttgc ctagcttcgt aacagagact atgagagaag
    35761 cgtttatatc tcttgataaa aaattgaatg ggtttgcaat gtatgatgct gttttgacgg
    35821 gagtagaaac taggtcatca tcaccaataa gaatagtaag agatgaaaat actttagaat
    35881 cagtgagtat taagaatcta tatccatgtg gtgaaggtgc tggatatgca ggtggtatag
    35941 ttacagcagc agttgatggt ataaaatgtg cagaaaaaat aatacaaaaa tacaccaata
    36001 atacaaaaat ttaacataaa cttaatgatt ttttaatatt taacatgaat ttaacattat
    36061 cactcctata atttgataaa atacaatagg ttataaaatt tttagataca gtaatctctt
    36121 tacaaggagg aaataatttg aacattataa taagtctaat gggtggatta ggtctgttct
    36181 tatatggtat gaaccttatg ggagaaggtc ttcaaaaatc tgctgggaca aagctaaaaa
    36241 agataataaa actacttact agtaacctat ttatgggagt tttggtaggt acaggagtta
    36301 cagcagtaat acaaagttca agtgctacta ctgtaatggt tgttggtttc gtaaatgcag
    36361 gaataatgac tttaaaacag gcaataggag ttataatggg tgctaatata gggactactg
    36421 ttacagccca attggtatct ttcgacttaa ctggtatggc cccagtagca ttaggtgtag
    36481 gtattatact ttatttattt ggaaataagc ctagaataaa aaatatagcc gaaatactta
    36541 taggttttgg tatattattt acaggtatgg attttatgaa gatggcagtg gagccactaa
    36601 gagactatca aggatttact gacttacttg ttacatttgg tagatatcca ctacttggat
    36661 tattattggg atttgggatt acagcaataa tacaaagttc aagtgcatct atgggtatgt
    36721 tggttgcact ggcagcagaa ggtctagtgc cattatcagc agcactgcca attctttatg
    36781 gccaaaatat tggtacatgt gtaacttcac tgttatcaag tattggtgct aacaaaaatg
    36841 cacgaagagc agctatgatg catctaatct tcaatgtttt aggaacggtg atattcttaa
    36901 tattcttgaa taaaccagta gtaagcatgg ttacaagttg ggacccaagc aatgttgcaa
    36961 gacaaatagc caatacacat acattgttta atataatatc tgtattgata cttttaccat
    37021 ttacaaatct tattataaaa ctagctataa agcttgttcc tgatagagca ggtgatatag
    37081 atgaagatga aactaaaact attaaatata tagatgatag aatgatagaa actccttcta
    37141 tagctcttgc aaatacgata aaagaagcac ttcgtatggg tgagaaggcg aaggaaagcc
    37201 taaatgcatc tatggaagct ttagtagaac attcaactga aaaaatagat aagacatata
    37261 ggcgagagag attaatcaat gatttacaga aagctatttt aaattattta cttaaacttt
    37321 caaaagcacc tttagatgat gattcaagag aagttgtgga tactttgttt aatacagtca
    37381 atgatataga aagaattggt gaccacgcta aaaatatagc tgaattatcg caagttgcaa
    37441 tagattcaaa tatttctttc tcagaagaag gacaatctga acttgatgtt atgtataata
    37501 gagttgtttc agcatatact tatgcattag aatcaatgag aactgataat gttgatttag
    37561 cttgtaaggt tataaaaatt gaagaacaag ttgatataat ggagaaatct tgtagagcta
    37621 atcacatgta tagattgaac aataatctat gtagtataga aaatggagtt atttatttag
    37681 acgtaataag taatttagag agaatatctg accatgctgt caatatagca caacaagtta
    37741 tagcaaagag attaggaaat gattaaaaat aattaattta aaataatata aaatttaaaa
    37801 tagaggataa ataaaagaga aactttaaat gatacgttta gagtttcttt ttttgtaaat
    37861 ttatttgact aaaattttta aatttataca tagttatagc atttagaaga atcgatttcc
    37921 tgataataaa tgatgagata tgtatgattt tgtggcaaac ttttaaaaaa aacacctata
    37981 ttttgtgtta taatctcttt aaaacgaata tgctgaaaag agggatttaa atgggaaaaa
    38041 taaattttga ttatagtaaa gctacagagt ttttttgtca aaacgaaata gatgtcatgc
    38101 aaccatatgt agatgttgcg cacgacatgt tacataataa aactggttta ggaaatactt
    38161 ttttaggatg gatagacctt cctaaaaatt atgataaaga agagtttgac agaataaaaa
    38221 aatctgctga taagataaaa tcagactcag atgttctttt ggtaataggt ataggaggct
    38281 cttatttagg ttcgagagct gctatagata tggtaagtca ttcttttagg aatggactta
    38341 aaaaagagca aagaaaggct ccagaagtat attttgtagg gcataatata agctcaacat
    38401 atattatgga tttattagat ataattgaag gaaaggatat ttctgtaaat gtaatatcta
    38461 aatcaggaac tactacagaa ccagctctgg cttttagaat atttaaagat tacttagaga
    38521 aaaagtatgg taaggaagaa gcaagaaaga gaatttatgc aacaacagat gcaagtaaag
    38581 gtgcacttag acagttagct acagaagaag gatatgagac ttttgtaata ccagatgatg
    38641 taggtggtcg tttttctgta ttgacagcag ttgggttact tcctattgct gctgctggac
    38701 ttgatattga tgctatgatg aaaggcgcaa atgatgctag agaagcattt caaaatcctg
    38761 acttaaaaag taatgatagt tatagatatg ctgttgctag aacaatttta catagaaaag
    38821 gaaaagatgt agagttatta gtgaactatg aacctcaact acattatgta agtgagtggt
    38881 ggaaacagct ttatggagaa agtgaaggaa aagaaaataa aggtttattc cctgcttctg
    38941 tagatttctc aacagattta cattctatgg gtcaatacat acaagatgga aaaaggcttt
    39001 tatttgagac agtacttaat gttgaaaact gtaagagaaa tataactata agttcagagg
    39061 aagtagactt agatggttta aactatttag caggtaagac tgtagatttt gtaaatcata
    39121 aagcttttga aggaactcta ttggctcata cagatggaaa agtacctaat ttagtaataa
    39181 atatacctca actagatgag tataattttg gatatttagt ttatttcttt gagaaggctt
    39241 gtggaataag tggatattta ttgggagtta atccatttga ccaaccagga gtagaagctt
    39301 acaagaaaaa tatgtttgct ttactaggca aaccaggtta tgaaaaagaa aaggaagagc
    39361 tagaaaaaag acttaaataa ttttaaatag ttaaggttta attaagatta aaataatata
    39421 atgaaatcat cgaaaaggag tttactcaaa atagatgatt tcattttttt tatgaaaaat
    39481 tgggaaaaat atattttgat aatttatatt atattaagat agggagtggt atgagatgtc
    39541 aagaagaaag aaagggataa gtttagtaat attagttgct ataataagtt caatacttag
    39601 tagttttcta acaataattt tagttaagga caatttagta agtaaatcta caggaagttc
    39661 tacgccgata gttgtaaatg atgatggtaa gagtcaaaat atttatcaag cagttgcaga
    39721 aaaagctacg ccatctgttg taggtattac aacaactagt gttgatacta gtaatatgtt
    39781 tgcaatacca actgaaacac aaggtgtagg aactggaatc atagttgatt caaatggata
    39841 tatacttact aattctcatg ttatatcaga tggtcaagct acaagtgtaa atgtattatt
    39901 caatgatggt tcaacaactt caggtaaagt agtatggttt gaccaacaac ttgatttagc
    39961 tatagttaag gttgataaaa ctggactaac accagcagaa tttgctgata gtgacaaagt
    40021 aaaagttgga gatatatcaa tagcaatagg taatcctctt ggattagatt ttcaaaaaac
    40081 agttactcaa gggataataa gtggattaga tagaactata caaacagaaa aaactaatat
    40141 gactggttta ttacaaactg atgcaagtat aaatgcagga aatagtggag gaccattgtt
    40201 aaatcaaaaa ggtcaagtaa ttggtataaa cacagcaaaa gcttctcaag cagaaggatt
    40261 aggatttgca ataccaatca atacagctaa gagtatagtt gaagaagtaa tcaaaaatgg
    40321 aaaatatgaa aaagtaactt taggcataaa aggtacagat gtatcaaatt atgaagcagc
    40381 aacaggtaca aaattaagta ctgataaagg tgtttatgtt gcagaagtaa tatctggttc
    40441 atcagcagaa aaagctggag taaaggtagg ggatattata actaaagtag gagatacaga
    40501 tataacaggt atgaatgatt taaataaaaa gttgtatact ttctcaaaag gtgctagtac
    40561 aaaaataaca gtaaatagag gcggaaaagc agtaacaata aatgtgaatt tctaaatatg
    40621 accagaaaga tagagactac atggtaaagt gtagtctcta tttttattta atttttatag
    40681 tatatacatc taaattaatt aaaatgatgt attgtttata gtttattgct atattttctt
    40741 ataaaaataa atttaataaa tatttaaatt taatttacaa aaacaatata aatatcataa
    40801 taaaataaaa ttttttaata ttactaaaaa tatatagaca aaataataac gaatatagta
    40861 ttatgtaatt gtagaaatgc ttttcatagg tatattctat gaagatgaat tgcataaaaa
    40921 tgaaaaaatg gaatggatat tatttagggg tgaaaaatca tgaatccatt agttataaac
    40981 ttacaaaaat gtagcataca tgatggacca gggataagaa gtacagtatt ttttaaaggg
    41041 tgtccacttg aatgtgtttg gtgtcataat ccagagagtc aaacatatac aaaacaagtt
    41101 ttgtataatg aagaaagatg ttcaaaatgt gaagcatgta taaatatatg ccctcataag
    41161 gcaatttaca aaggtgaaac aaaaatatgc ttagaccaag ataagtgtga gttttgtgaa
    41221 acttgcttag actattgtgt aaacaatgca agagagattg ttggtcaaga gtattctgta
    41281 agagatttgg ttaaagagat agagaaggat agaattttct atgaagaatc aggtggtgga
    41341 gtaactctat caggtggaga agtaatggct caagatatgg acttcatttg tggagtaata
    41401 aacatgtgca aatcaaaggg aatccatgta gcaattgata catgtggcta tgcaaagtca
    41461 gaaaattatg aaagagttgc taaatgtgca gatttgtttt tatatgatat taagcttatt
    41521 gatgaagata agcacataaa atttacagga aaatcaaatg acttaatact gaaaaatgtt
    41581 aagattttaa gtgaattagg ggctaacata aatataagaa taccattaat tgtaggagta
    41641 aatgtagatg atgaaaattt agaggttaaa aaaatgatag agtttttaaa gcctttgaat
    41701 atacaagcag taagtcttct tccatatcat aatattggta aacacaagta tgataaaatt
    41761 tataaaaagt atgaaggaga agaactacaa agaccatcag aagaaaaatt agaggaaata
    41821 aaaagactgt ttgaagcaag taatttcaat actaagattg gagggtaata aaatggcaag
    41881 aggaactttt gagagaacta aaaaattaag agaagaaagt attaatgcag aaccacacat
    41941 atctattgaa agagcagtac ttatgacaga agcttataaa aaatatgagg gaagtgtaga
    42001 gattcctgta cttcgtgctt tatcatttaa gcattatatt gagaatagaa ctcttagcat
    42061 aaatgatgga gaattaatag taggtgaaaa aggtgattct cctaatggag caccaacata
    42121 tccagaaatt tgttgtcata caatggaaga tttagaagta atgcacaata gagatataat
    42181 aaattttagc gtatcagaag aagctagaaa aatacataaa gaagaaataa ttcctttttg
    42241 gaaaaagaga caaacaagag ataagataat aaatgcaatg acacctgagt ggttagcagc
    42301 atatgaagca ggtatgttta cagagtttat ggaacaaaga gctccaggac atacagtttg
    42361 tggagataca atatataaaa aaggattttt agatttaaag aaagacatag aagcaagatt
    42421 aaaagagcta gattttctta atgatttaga tgcatataat aaaaaagcag atttagaagc
    42481 tatggcaata gcttgtgatg caatggttat attaggtaaa agatatgctg agaaagcaag
    42541 acaaatggca gaagaagaaa cagatgaagc taagaaaaaa gatttattac taattgctga
    42601 aacttgtgat gttgtaccag ctcacaaacc agaaacttat catcaagcta tacaaatgta
    42661 ttggtttgtt catatagggg ttactactga acttaatata tgggatgctt ttactccagg
    42721 aagacttgac caacatctaa atcctttcta tgaaagagat gtagaaaatg gaatactaga
    42781 tagagataga gcacaagaat tacttgaatg tttatgggtt aaatttaaca atcagccagc
    42841 accaccaaaa gttggtataa cattaaaaga aagtagcaca tatacagatt ttgcaaatat
    42901 aaacactggt ggaataaatc cagatggtca agatggtgtt aatgaagtta gttatataat
    42961 acttgatgtt atggatgaaa tgaaattgat acaacctagt tcaaatgttc aaataagtaa
    43021 aaagactcct caaaaattct taaagagagc ttgtgaaata tcaagaaaag gatggggtca
    43081 accagcattt tataatactg aagcaatagt tcaagagctt atggaagctg gaaaaactat
    43141 agaagatgca agacttggtg gaaccagtgg ttgtgttgaa actggatgtt ttggtaaaga
    43201 agcatatgtt ctaactggat atatgaacat tccaaaaata cttgaattaa cattaaataa
    43261 tggatatgac ccaattagta aaaaacaaat aggtattgaa actggagacc ctagaaattt
    43321 ccaatcttat gaggaactat ttgaagcatt taaaaagcaa ttacattata tgattgacat
    43381 aaaaatagaa ggaaatgctg tgatagaaaa tatatgtgca aaacacatgc cttgtccact
    43441 tatgtctaca atagttgatg attgtataga aaaaggcaag gattaccaaa gaggtggagc
    43501 aagatacaac actagatata tacaaggtgt tggtatagga actataacag atagtttaac
    43561 agcaataaaa tataatgtat ttgataagaa aaaattcgat atggatacat tgcttaaagc
    43621 acttgatgct aattttgaag gatatgaagc tatacttaat ttagtttcaa ataagactcc
    43681 taaatatgga aatgatgatg attatgctga tgaaataatg caagagatat tcaatgcata
    43741 ttacaatgag gtaactggta gaccaactgt ttgtggtgga gaatacagag tagatatgtt
    43801 accaacaact tgtcatatat actttggaga aattatggga gcaagtccaa atggtagact
    43861 ttgtgcaaaa ccagtttcag agggaatttc acctgaaaaa ggtggagata ctaatggacc
    43921 tacagcagtt ataaaatctt gtgctaagat ggaccacata aaaactggtg gaacattatt
    43981 aaatcaaaga tttgcaccat cagtagttca aggagaaaaa gggttagata atatggctaa
    44041 tctagtaaga gcatacttta atatggatgg tcatcatata caatttaatg tatttgataa
    44101 aaatgtactg ttagaggctc aaaagaatcc tcaggattat aaagacttaa tagttagagt
    44161 tgcaggatat agtgaccatt tcaataattt aagtagaaca ctacaagatg agataatagg
    44221 aagaactgag caaacattct aggtttgctt gtaaataaaa cttcttacta tataaaatcg
    44281 taactgatat aataaagtat aaatggtttg attccatttt taaaatttta gattaattat
    44341 gagctaactt tctaaatagg aagttagctc gttaagttta atgaaaaaat cgttaaattt
    44401 atatttagta aatcatacac atattagtaa gtaatacata caaaagaata atttattata
    44461 gttataataa ggttatattt tataaaaata agataatttt aagcagttaa aataaagtca
    44521 atttagctac tttaaaataa aactaataga aaaaaggaga tttgatatga aaactttagg
    44581 atttattggt tcagggaata tgggaagtgc aatgataggt ggcatagtac agtcaggatt
    44641 agtaaaggca gaagatataa ctgtatcaga tttaagccaa gttgccttag ataaagtaaa
    44701 agaaaaattt ggagtaaata ctacaactga ttcaaaggta gctgttacaa attctgatgt
    44761 ggtaatagta gcacttaaac ctaatatatg cgaaaaggct ttaactccac ttaaagaatt
    44821 aatagatgca aataaaataa tagtatctat agcagcaggt aaaactatag aaagtctgga
    44881 aagttttata ggttcagata aaaaaatagt aagagtaatg cctaatactc cagcccttgt
    44941 tggcgaagga atgtcagctc tttgtaaaaa tagtaatgta acagatgaag agttaaatat
    45001 gattaaggct ctgtttgaat cttttggtga ggcagaaata gtatcagaat atttaatgga
    45061 tacagttaca ggtattagtg gttcttctcc tgcatatgta tttatgttta tagaagctat
    45121 ggcagatgca gcagtattag ctggaatgcc aagacaacaa gcttataaat ttgcatctca
    45181 agcagtaatg gggtcagcta agatggttct tgaaactgga aaacatccag gtgaattaaa
    45241 agatatggtt tgttctccag caggaactac tatagaagct gttaaggtat tagaagaaga
    45301 aggctttaga gcagcagtta taaaatctat tgttgcttgt atagataagt ctaaagatat
    45361 gagcaaataa cagatgtaat taagtttact atatggtttc ttgaattgtt tttttagtgc
    45421 ttatttatat ctatacataa atgtatttgg tataagtaaa ataagagtaa tttactataa
    45481 aaaggaattt a
//
LOCUS       DAMJVK010000023        44901 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.023, whole genome shotgun sequence.
ACCESSION   DAMJVK010000023 DAMJVK010000000
VERSION     DAMJVK010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 44901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44901
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.023"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            60..773
                     /locus_tag="PC414_001255"
     CDS             60..773
                     /locus_tag="PC414_001255"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012301.1,HMM:NF016296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2463660.1"
                     /translation="MIITICENDDMQRDIIYDFVKIFLTKIKVKHYDILMFKNGEELL
                     EEYPNKVDLILMDINLGGRNGFDISKDIRKFDKDARIIFVTVSEKYAIKCYEINAFRY
                     MIKPIYYKEFEKYMLDFAIDWRKREAIITIKRKKQIIKIEEHNILFVEIVNRKLRVVT
                     KNEEHIINDKMDMFEEKLKEDIFFRCHKSYLINLLEVTNFNYNFVYLTSEKIKIKVAP
                     KKTKKLKEKLANFFREIIW"
     gene            complement(1237..1590)
                     /locus_tag="PC414_001256"
     CDS             complement(1237..1590)
                     /locus_tag="PC414_001256"
                     /inference="COORDINATES: protein motif:HMM:NF024252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2463661.1"
                     /translation="MNENLKELRIKLGLSQKEFAEKLLVSDATISRLEKGERKLTDRF
                     IFQICEVFNVNEDWLRNGNGEMFKNIDDIELAAMMGKTFSSNDEFLKKVFLTFAELTD
                     DEREVIQKVIDKLSK"
     gene            1770..1940
                     /locus_tag="PC414_001257"
     CDS             1770..1940
                     /locus_tag="PC414_001257"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463662.1"
                     /translation="MYNNNDKKLINLFESFKKIDYTEQSLVYFLSLSFIVNRKCHKDA
                     NLYSRRGECNYE"
     gene            1933..2739
                     /locus_tag="PC414_001258"
     CDS             1933..2739
                     /locus_tag="PC414_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HDF2463663.1"
                     /translation="MNNLNIKGKQKFMDLEIPIIEGGFGEGKKCITDKTISEIHNMEI
                     KHVRELINRNIRRFINAIDFIDLKVVVDNDYNLEVLELLSYRKMQISKANNIYLLSER
                     GYSKLIKIMDTDLAWEIHDKIMDEYFNMREQLIDSYMIQDSIERAKRWIEEEKERLLL
                     KETIQVQQPKVEYHDKVLNPDKLVTTTDIAKDLGMTAQALNKLLHLNRIIYPERINGR
                     IKCWKPYKNYLWLIPEYADFKITEHDTQLKWTEIGRKWLIEFVENIKKIS"
     gene            2803..3027
                     /locus_tag="PC414_001259"
     CDS             2803..3027
                     /locus_tag="PC414_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463664.1"
                     /translation="MIKQILNKRGSLLNLASEYLTEQDYLKFQGWYTHRYSKLDAYDA
                     SINEYLNYKNDKKLITYVFNLKFQEALIEI"
     gene            3051..3587
                     /locus_tag="PC414_001260"
     CDS             3051..3587
                     /locus_tag="PC414_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463665.1"
                     /translation="MNYNYVTKIDLKYGNTQFGFEKYSDALDKYRELKEEFKYVPCII
                     KLHSYVAHQDLYVKEIKADGFEKKYLDIKNLIKLFKEHKQNISDELSLCDKKKEEVLH
                     VIEEMNLDTASLNDKIDTIKELKNTLVKRRLVKYELNKYFAFCNTLYEIEEELKKYES
                     KYKQKENQGKSRFKKNKK"
     gene            3596..3736
                     /locus_tag="PC414_001261"
     CDS             3596..3736
                     /locus_tag="PC414_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463666.1"
                     /translation="MEEYHFNLIKKLCAERKIHKGWFRNIEKCGCGGKCNKKNCKMLE
                     KL"
     gene            3747..3980
                     /locus_tag="PC414_001262"
     CDS             3747..3980
                     /locus_tag="PC414_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463667.1"
                     /translation="MENQIELTEKDIENTLSALNIIKYICIKSKHCKNCPLGYMKNLD
                     EHNCMLYELIDKGLLPCDWSVKKVTRLLDTTEE"
     gene            3999..4412
                     /locus_tag="PC414_001263"
     CDS             3999..4412
                     /locus_tag="PC414_001263"
                     /inference="COORDINATES: protein motif:HMM:NF021182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463668.1"
                     /translation="MELDFRIWNGFRMIHGEELRLLTEEMFIKSGYSEQYYNDICIPR
                     VEDLKIMQYIGLKNCFEKKIFQYDIVKFIFDGVWKVCLVNLSLPKGATVTFESIGGKY
                     TFNLSEIFSKDRKLEIIGNFYENKDVIKKYNLSIE"
     gene            4422..4616
                     /locus_tag="PC414_001264"
     CDS             4422..4616
                     /locus_tag="PC414_001264"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463669.1"
                     /translation="MDEMIIRVKMGEPLPTLGKIIKCTTCLTNEEKCFKVNKICKLSW
                     INDRNSEGLIVECKGKYIKY"
     gene            4823..5377
                     /locus_tag="PC414_001265"
     CDS             4823..5377
                     /locus_tag="PC414_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HDF2463670.1"
                     /translation="MSKKDKLSIFISLILRHKPETIGIKLDDYGYADVNELIEKINNT
                     GRNINIEILEQIVKEDNKQRYSFNDDRSKIRANQGHSINVEVELKELEPPKCLYHGTA
                     TRFLENIKKEGIIRQSRLYVHLSNDIDTAVKVGKRHGVPVVLKINTGKMYENGYKFYL
                     SENNIWLCKYIPFEYVEKISRVVK"
     gene            5404..5730
                     /locus_tag="PC414_001266"
     CDS             5404..5730
                     /locus_tag="PC414_001266"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463671.1"
                     /translation="MDFNFRKICEKDELTDDFIIKICQLSHEEFGGTIYVPCNIHPCG
                     WDELEVPNPKDLDSDKLMEEFLLEYKFECENISSNIYDSDHPFIGKTLREILMIVDCR
                     FIIGVI"
     gene            5745..6521
                     /locus_tag="PC414_001267"
     CDS             5745..6521
                     /locus_tag="PC414_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463672.1"
                     /translation="MENIELTPRDYFEDIKNKKQKNTNENLDIVYENSLILLNKYIVT
                     GQKKGAKKLMFLLECIEKERNVIELGIDTFVYKEDIEKYIDDISKNTVKIIELENYER
                     EIPDEIVEIIEKTKDIFDKMYVLFTDYTGKITKEISKERRDKDPILFGVFSDEKSRAI
                     VDRFYYLGDWEDEYCDLTLEKMINDYKKKGRQDILKHIITPKSLDELKKELDSLEKNN
                     DTYKQVANIKPLPTTKNSSVEPPKLNIFKKIKNRVFKNEK"
     gene            6511..6912
                     /locus_tag="PC414_001268"
     CDS             6511..6912
                     /locus_tag="PC414_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463673.1"
                     /translation="MKSNVDLTENRDFRDVRGRGINILGIEDAPNRMLSFSDFGIGRL
                     YGKNLEFEFNKNEVFLTGNANDREEKKFLILCEENKVCVRCGDELREGWDKVSIELCN
                     ECYEMLDVEYKNKKIIRELFFRDRDVNIELL"
     gene            7101..7421
                     /locus_tag="PC414_001269"
     CDS             7101..7421
                     /locus_tag="PC414_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463674.1"
                     /translation="MFIKSFRCIFRHKFSRIKDCQLITTRSQQYTCKIWMCKYCGQIV
                     AGSTPVYHPNMKVNEYCVKCEFSACANNYEGYCNSKDKEIEFKSICLKGNKEILQCKN
                     FDLE"
     gene            7452..7637
                     /locus_tag="PC414_001270"
     CDS             7452..7637
                     /locus_tag="PC414_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463675.1"
                     /translation="MKINVHVKTNKIGSDCELEFEIDDDDLKDMSDEEREGYIDKIAW
                     DYVFENFIDWNWYEVED"
     gene            7692..8093
                     /locus_tag="PC414_001271"
     CDS             7692..8093
                     /locus_tag="PC414_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HDF2463676.1"
                     /translation="MKELLEVKKIFDSLASTSSRKEKERILEKYKNNRMFVECLQFLL
                     DSNILTGISKNKICKNLNNTGHNELENIYDMLDYLIKNNTGRNIDVKTIQVFASKDEK
                     LKDFIFNLATKSIKLGITYKTVDKIMPGLII"
     gene            8195..8698
                     /locus_tag="PC414_001272"
     CDS             8195..8698
                     /locus_tag="PC414_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463677.1"
                     /translation="MTDKIIIASVDGEEMIFRTEMKKLEKGDSLIVSVNGVKRLALFI
                     RYISTCEEYIEDIAFQKVDEILYENTIAKEKERVKKVFKDKCMKNYKPIVNTEIDSYL
                     RECGFNCDGRQFFKSIDNTQILISRDCDKDVLTVSKGLTITKTDITNKNLITVIKIIN
                     NSLLIVR"
     gene            8758..10983
                     /locus_tag="PC414_001273"
     CDS             8758..10983
                     /locus_tag="PC414_001273"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside triphosphate reductase"
                     /protein_id="HDF2463678.1"
                     /translation="MKNVIKRDGRIVPFDEKKIINAIEKSFLSLRNKGKSEVFDRELA
                     TLIAKKIKNKPCEKMHIEDIQDIIEFSLMASKKKDVAKEYITYREKRNIARGRKTYND
                     YMSIVNTESNDITKENANMNADTPAGMMMKFASEATKTFTDHVLLSEEAKEAMMDNYI
                     HIHDKDYYPTKSLTCIQHPLDKIFNNGFRAGHGSSRPTKRIETSSILGCISLETVQNE
                     MHGGQAIPAFDFYNAPSVRKTFIEEIKNIEIKDDIDLSHLYNIEIEDYIIKDLKELDS
                     EKRNVQHAINRTVWRVHQSMESFIHNMNTIHSRGGNQVVFSSINYGTDTSAEGRCIIR
                     EILKSTYEGVGNGETPIFPIQIWKVKEGVNYNPTDRNYDLYELACKVSAKRFFPNFLN
                     LDATFNKHVKWKMCDPNRYIYEVATMGCRTRVFEDLHGEKTSIGRGNLSFTTLNLVKM
                     ALEAKEKYPNNEANRVAEFIFLLDKYSDVVARQLYDRYLFQCTAKAKQFPLLMSGLWK
                     NSENLNPNDDVSEVLKHGTLGVGFIGLAECLIALIGEHHGESEKAQKLGLEIISLLKS
                     KVEKYKKKYNLNYSVLGTPAEGLSGKFTQKDKKIFGEIKNITDKDYYTNSSHVPVWYN
                     CNVEHKAKIEAPYHELEGGGHIFYVELDGDATHNPKAIMKVVDLMKKYNMGYGSVNHT
                     RSRCMNCGFENADSELKECPKCGSNSINIIQRITGYLVGTTDSWNNAKLAELKDRVTH
                     G"
     gene            10976..11473
                     /gene="nrdG"
                     /locus_tag="PC414_001274"
     CDS             10976..11473
                     /gene="nrdG"
                     /locus_tag="PC414_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HDF2463679.1"
                     /translation="MDKDYIYILDIKHDTIVDGEGFRTSIYCSGCNHMCRGCHNPQSW
                     DIKNGTLTKVLDIYKEIISNKFSDVTFSGGDPLLQLQGFVHLAKLIKKNTNKTIWCYT
                     GFKFEELITDNEKLELLKLIDVLVDGKFEEDKKSLELIFKGSSNQRIIDVQKTLYEKQ
                     IVLYE"
     gene            11674..12081
                     /locus_tag="PC414_001275"
     CDS             11674..12081
                     /locus_tag="PC414_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463680.1"
                     /translation="MYFETDIIKNKNTVNKIIKYCGGNTEKLKDWLKEIENKSISSLE
                     ELKEYPLKRNYKFVFSKEEAPNLDKLIKMKLRDNCLEKCEICSLENLLSMLNDEILDL
                     NKEDISYEDNIEWIENCKEELIKLKVGSICIDW"
     gene            12102..12413
                     /locus_tag="PC414_001276"
                     /pseudo
     CDS             12102..12413
                     /locus_tag="PC414_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900673.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12504..12854
                     /gene="tnpA"
                     /locus_tag="PC414_001277"
                     /pseudo
     CDS             12504..12854
                     /gene="tnpA"
                     /locus_tag="PC414_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003378761.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            12872..14311
                     /locus_tag="PC414_001278"
     CDS             12872..14311
                     /locus_tag="PC414_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015978935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2463681.1"
                     /translation="MSNYILTLQLKTEKFQEDIICNAFDKYRKVYNSCLSELFKRYNH
                     MIESKEHQANCKYKGKDRNKIFSEINKKYNLTEYSLHKFVKFIGKYYKLHSAITQKIA
                     TRCFNAFNKYIYHQAKRVNYIRYNELTSIEGKQNSTGISYKDGIIKFNKMTIPVIIKN
                     NDNYAQRAIQDKIKYCRILKKEIKGKIKWYVQLVLEGIPPKKTTNQGKIKGQIGLGNV
                     GIDIGTQTIAISSKCDVKLLELAPNVNNIDRQIKLIQRKMDRSKRATNLNKFNDNGTI
                     KQGNRDKWIFSNHYLKLKRLRKELYRKQAEIRKQDHYILINKLLNLGNKFYVETMSYK
                     RLQTRVKDTTINEKTGRINKKKRFGKSLANKAPSMFLTMLDNKLKYNNERLYKIDTAK
                     CRASQYNHFSDEYNKKELKDRWNNGMDIQRDCYSAFLIMNVNEDLKSINRELCVKTYD
                     NFKILHDKEINRLKKLKLNGYKLISSMGI"
     gene            14512..14736
                     /locus_tag="PC414_001279"
     CDS             14512..14736
                     /locus_tag="PC414_001279"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463682.1"
                     /translation="MVIYKNKKKDIIRDESMYDYGMSLSSIMKVILNHQEDIEKLKEE
                     EVAEFIKNKLENEHDCDELKIISEVFNEIY"
     gene            14723..14833
                     /locus_tag="PC414_001280"
     CDS             14723..14833
                     /locus_tag="PC414_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HDF2463683.1"
                     /translation="MKYIKHVDNGVMRAIGIDNYKPLLNIIKEKVNLESN"
     gene            14862..15329
                     /locus_tag="PC414_001281"
     CDS             14862..15329
                     /locus_tag="PC414_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463684.1"
                     /translation="MGLDLGFYSVKTKVTDLDIYLNKTRGEKYKELNNDEEEFYKFEE
                     LLDIDISLEDWSVIRMIYKAKWEKGYESYEDEAILITKEDLEDYIIPFLKQKDINSKI
                     YNKDKEYYYKNDKKVTVDDRYTQEFWDLVLKEFQKMLNIIDFEKHSLIIMYWY"
     gene            15365..15745
                     /locus_tag="PC414_001282"
     CDS             15365..15745
                     /locus_tag="PC414_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-tRNA hydrolase"
                     /protein_id="HDF2463685.1"
                     /translation="MKKMYIAINSDLNMSPGKVGAQVAHAVYDYLYNKILDTVSCSYE
                     TEIIAHELIDLKCDLTSFKNNGDTICILKAKEAQLLKFKEKGYLTIVDRGLTEIPKNS
                     ITCVNLGIFNEDEIPSTIKRLRLY"
     gene            15761..15916
                     /locus_tag="PC414_001283"
     CDS             15761..15916
                     /locus_tag="PC414_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463686.1"
                     /translation="MYSKGDVVKVWCYQCERIEFQEFVGYFNSYFDNYKLKKRWKCSK
                     CDVINNF"
     gene            15943..16389
                     /locus_tag="PC414_001284"
     CDS             15943..16389
                     /locus_tag="PC414_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463687.1"
                     /translation="MKIKKIILGLDNLENVELGIGDVGGFYLDNIHEIIGGFALSYIG
                     KCKKADYLHIQIHKRVDEIQRLHDYKDITNVKIEYEDGSVEDIYVPYKQEGDFLGLDN
                     LYQTSFISKQGYLHIVISKDKTVEEVFNEEFEDDEGVEFSFNMYGI"
     gene            16667..16831
                     /locus_tag="PC414_001285"
     CDS             16667..16831
                     /locus_tag="PC414_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463688.1"
                     /translation="MTDKEMEEYIINLIKDKIKEEYGENILPEILMEKFIKLERKGSV
                     TVDVRDLIEG"
     gene            16875..18128
                     /locus_tag="PC414_001286"
     CDS             16875..18128
                     /locus_tag="PC414_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HDF2463689.1"
                     /translation="MEVQGKYNKAKIFTENVDSITIGQIINLCNQKEFKESSIRIMPD
                     CHAGKGCTIGTTMTISDKVVPNLVGVDIGCGMATIPLEKYINNINYTKLDKIIRDYIP
                     HGFKIHNKSREKLLKNLFNIDINNLRCEVNKNRAYMSLGTLGGGNHFIEVDKSEKGQM
                     YLTVHTGSRNLGKQIADYYQDKAIKYCIEKYKKSYDDAKEFLISTLKKDNKEHLINKY
                     LKDLSDRKLNKPHDDLCYLENDLMEDYLHDMSIAQKYAAANRMVIIADILFKYNSLEV
                     NYDHFIRDIKCDVIECIHNYIDMDSKILRKGAISANKDETVIIPVNMRDGIILGKGKG
                     NSEWNYSAPHGAGRILSRGKAKEKISLDEFEESMKEIFTTCVGQSTLDEAPQAYKPIE
                     DILNNIGDTIEIMEVLKPIYNFKSN"
     gene            18170..19072
                     /locus_tag="PC414_001287"
     CDS             18170..19072
                     /locus_tag="PC414_001287"
                     /inference="COORDINATES: protein motif:HMM:NF014342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463690.1"
                     /translation="MIKLITHNDLDGVSCCIIAKYILREQIDVNYCNYKNINDVVLDT
                     LKNHDKYEQILITDISVNEDVAKKLDRIKNKVQLLDHHPTALFLNNYDWANVEIECNK
                     GKTCGTQMLFNYLQRYYDDTSESTLKYFIELVRRYDTWEWKDKYNDLDAKYLNDLMYI
                     MGIEDFIMDMLTKIDSDEMCLFDKLSLKLLEIKQKEIDRYVEKKDENIMVKEILGYNA
                     GIVFAENHISELGNKLSELHPELDFIVIINREIISYRTIKDNINLSEIAKVFGGGGNP
                     KASGSQIDESCIDNYIKDIFNIKS"
     gene            19180..19458
                     /locus_tag="PC414_001288"
     CDS             19180..19458
                     /locus_tag="PC414_001288"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463691.1"
                     /translation="MMIDKFNYELYNEFPHLSPIIELYNLNIEYIREVVSILINTDMT
                     FQRFKYSKSRVECSMKAYIISPNSKLSVVFAYDCELNGIDLDNLKAII"
     gene            19505..19915
                     /locus_tag="PC414_001289"
     CDS             19505..19915
                     /locus_tag="PC414_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463692.1"
                     /translation="MCKFCESIIDNTKDILLTSRSRLLEDNTCEIIAEDNCNNCYDGC
                     YEYFKLNGYKIRGNTYVNVDYYKKIRKIIIAPCSESVHINYCPYCGKQVSKDIKDFNC
                     IPEHIVDVRYKNGDVYDYDMEKSVQEVMDNANNN"
     gene            19946..20119
                     /locus_tag="PC414_001290"
     CDS             19946..20119
                     /locus_tag="PC414_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463693.1"
                     /translation="MNLWSKIEEPVMRAVSGIVFNIWGNVCFSIYYIEENKQIELEIY
                     DRPYRYEGKKNSC"
     gene            20229..20372
                     /locus_tag="PC414_001291"
     CDS             20229..20372
                     /locus_tag="PC414_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463694.1"
                     /translation="MNKGIKNYITAVNYLLDNLEYMMEDDEFNNEKLQKRIDVIRKFE
                     REL"
     gene            20374..20679
                     /locus_tag="PC414_001292"
     CDS             20374..20679
                     /locus_tag="PC414_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463695.1"
                     /translation="MGKIKNGEIDTEDYRLFKNIQKEKDRVYSKYINEEYIMEEFINY
                     LRLEDKDYTTEILERYARYIDENLDNRCPYCVYNICSEHDNCFDGILENLKEKIKIK"
     gene            20837..21037
                     /locus_tag="PC414_001293"
     CDS             20837..21037
                     /locus_tag="PC414_001293"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463696.1"
                     /translation="MIKERFKAKDSFNMDEFTKIWLMDFKEDFDLSLKKMIENTIKKS
                     IKDTYEETERAITNIENLINKL"
     gene            21242..21526
                     /locus_tag="PC414_001294"
     CDS             21242..21526
                     /locus_tag="PC414_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5052 family protein"
                     /protein_id="HDF2463697.1"
                     /translation="MKKFLLTGLLGTMLIAMSGCTGIQSSMKDAESDVSGLNRTVNVY
                     SDNGEVLKTYTSKSMRVKDGDGGTITLDFDGKRVIICNAHVVIEENSSNK"
     gene            21884..22036
                     /locus_tag="PC414_001295"
     CDS             21884..22036
                     /locus_tag="PC414_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463698.1"
                     /translation="MKITISIEEAMDIIKQHYENKGIEIKDLSFNLEKTGMVRNIKFK
                     GEIILK"
     gene            22070..22669
                     /locus_tag="PC414_001296"
     CDS             22070..22669
                     /locus_tag="PC414_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463699.1"
                     /translation="MFISSTNRSDARSFHISDYYRTPITKIKEFLTEFSRYENILNSN
                     IKILDPCAGGDNTNPMSYPTAIQEFSNQEISIDTVDIRDDSRANIKQDYLKFQPKEKY
                     DVIITNPPFNLSLDIINKALNDVREGGFVIMLLRLNYLGGKVRQRLWGNNMPKYIFVH
                     NRRMSFTDDGKTDSIEYAHFVWQKGYNPKFSQLKVLISQ"
     gene            22695..22907
                     /locus_tag="PC414_001297"
     CDS             22695..22907
                     /locus_tag="PC414_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463700.1"
                     /translation="MEINRRRCEYAARTGRYPNSLILWEGYYSLLNIQYIEDKTRGYI
                     TKFNGMDVIKCNDFLKIELFEKNINN"
     gene            22936..26007
                     /locus_tag="PC414_001298"
     CDS             22936..26007
                     /locus_tag="PC414_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HDF2463701.1"
                     /translation="MRYNNYHSHKIYSNIRSLDVITKPQQYIDRAIKLGQNTYFTTEH
                     GYQGNVYEAKTLCDEYKLKMIIGAEFYYVNDINEKDRGNYHLIVIAKNNDGYKQINKA
                     LSLANKNGYYYKPRIDEKILFEIFKPGDVVITTACVAGILKLENREELILKLKNYFKN
                     NFFLEVQSHPHKTQALHNKDVLELSNKYNIDIIHANDSHYIYPEESKYRTKFLKAKGI
                     NYPEEDGFILDYPNSEDIFKRYEKQNILTKQEVERALKNTLIFDECEEITLINDDIKL
                     PSISKNPNKELKEILNKEWLEKRKNIPKNRWNEYLDAIRYEFDIIEKTHMEDYFIIDY
                     KIVQRAKQEYNGLLTKTGRGSAPSFIITNLLGLTEIDRLNAPVPLFPTRFMSVERILS
                     AKSLPDIDLNAEDAEPFIQSTRDLLGEENCAWMISYKPLQDASAFRLWCKANDMKLSE
                     YDEVAKNLDKYTNDVFWKDVIKESKVFVGVVESVSFSPCSMLIYDRPIDEEVGLLKTK
                     DGVCCNIDGYYCDKYKYLKNDYLTVKVWSLIRKICELANISIPTIEELNILLDAKTYD
                     IYKDKLTCTINQVDSDYATNLASKYKISSVAETSAFVASIRPGFASLLDNFINRESYT
                     TNVKELDILLEDSYHYLMYQESIMKYLIWLGIEESESYDIINKIKKKRFKEKELKELH
                     IKLKNNWIKKVGTENNFEDTWQVVNDAASYSFNASHSLSYAYDSLYCAYLKSHYPLEY
                     YTVAFNLYNEDTERTRKLTDEIEYFSIKLENPKFRFSKSEYFFNRDTNSIYKGIESIK
                     FLNSDIGEYLYSLKDNKYDSFLELLIDLQGHINSKQLSILIKLDFFEEFGKSNKLLET
                     YDIYNSIYSKKQFKRDNLPCDINTMRKYSNKETEKIFKEVDTKELCSYLESQIENTDI
                     PTNEKIQAHFEFVGSCNIKDSNSNPRYCLVIDIDTKYSPKVTLYNISSGNIKIFKVSK
                     PIFEDNKIDVYDLVYLKSIKEKAKSKKVDGKWIRSNTETEWWIQEYWKIEN"
     gene            26110..26361
                     /locus_tag="PC414_001299"
     CDS             26110..26361
                     /locus_tag="PC414_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463702.1"
                     /translation="MISILIDEQRYYSLLEEKKDLSMRIDELKEENAFLKQENEEYEE
                     ILNEWECVRKEIVATCKKKEILGEKPSELEMVLQKIEDI"
     gene            26361..26681
                     /locus_tag="PC414_001300"
     CDS             26361..26681
                     /locus_tag="PC414_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463703.1"
                     /translation="MSVNKIYEKKEYIIIKSKSGYIVYNTNKEFKEGHTHLKSYNAAK
                     TAIDLVIKRRLPKSYSIYFLTSLMRISNDVQYIKHISDIVYIRNNKGLKEKYINSKNI
                     KKNY"
     gene            26756..27877
                     /locus_tag="PC414_001301"
     CDS             26756..27877
                     /locus_tag="PC414_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfJ domain-containing protein"
                     /protein_id="HDF2463704.1"
                     /translation="MKSYKEKQFLVFEFEDGKNVKYNLATGEYVGKSGRIVKDVCTQL
                     RGYNLHEVINSFEDENYKCFLNFVDGRVNRSKSSRAWGRRVERIRNIGSFLNEINNYS
                     EYEQLFSAGLKKIIYPIHCDIKSIPKGLISICRNYSITLSDRLIQGYSKNPNLYNNLL
                     SLELNSIRKTNLISILDSGYDRMIFDRLIDIYKYKPQSLINYIDNLMTYEALNGLEST
                     LNELYDYVVMMSVISDKYEKYPRHFLTTHKIASRNYNRLKTQFEEEVFNKRIDKSLEY
                     SYQDYKFIYPKNTNEIKDEAVQQNNCVSSYIQNVIDGKCHILFMRKKDNPSKSLVTIE
                     VRNGKIVQAKGKYNRDVNEREQEAIDKYNNRIERMKKIC"
     gene            27871..28314
                     /locus_tag="PC414_001302"
     CDS             27871..28314
                     /locus_tag="PC414_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463705.1"
                     /translation="MLIEGTKIRLMKEIQGFEMLKIGDIFVITSVGNNGAIHFKTNYG
                     MGFMNYSELKKYFEVAIETKKKEHIWSEWRTATVFGTNKTCTYRTNGKKVEVRMGDFK
                     ASATCHDSDEFELNKGVELCLARIEVKKANKQVNLVLDKINNKEI"
     gene            28324..28479
                     /locus_tag="PC414_001303"
     CDS             28324..28479
                     /locus_tag="PC414_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463706.1"
                     /translation="MSCKFKERCENKNRDCDYCCYNPNAYLEDAFEWNGEGRKPTDEE
                     LDDALEE"
     gene            28523..29083
                     /locus_tag="PC414_001304"
     CDS             28523..29083
                     /locus_tag="PC414_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463707.1"
                     /translation="MRWRNILVTSVLVMGSSIHIFANTKEMSTSRMDKNIVADTAKNI
                     NLMYKYEWEQHKNSSEYKLEIKRINLEKDLGIKIEKLIPVTWEVSYYTSLNCENSKYG
                     AITATGEKLQYGFVANNYLKFGTKILVDGNLKVVKDRGSNKYFGNSNAIDVFVPKLEG
                     ESDYKYYKRVNNMGRHYKEGYIIVEG"
     gene            29095..29763
                     /locus_tag="PC414_001305"
     CDS             29095..29763
                     /locus_tag="PC414_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463708.1"
                     /translation="MNVNGIKIPGIIDVSNVYVYGIKESIIASGYPMYEQQVIDMKKI
                     NISEKDLKRANQLGKSKQGSGHDCFSKGIIIQMDLQWSEYMWRQWDRYHHNDYVSSQS
                     KMHRLSKFNLDDMCNKYVDKNTINFLNELIHEYNNFEELKLNNSNNIILRNGEKVEVT
                     KETLWKIIISNCPSGLMLTARVTLNYLQIKSMINQRENHKMEEWRILCDFFKTLPKYK
                     ELMM"
     gene            29809..30702
                     /locus_tag="PC414_001306"
     CDS             29809..30702
                     /locus_tag="PC414_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463709.1"
                     /translation="MAEKRINYNQVFVSGEVLDILNIARMNQGTGQEAIRFTLKVETA
                     PNESVNVDYFTSMFKSDGSPNQMFLGIETIANEIKTRAEDGKGDIVRCVCSLDNNLYY
                     KDGEKKERFQISGTFCNREKYDDKGNPIDDKGEVVKSIKASQVWRVYTLIENIEEKED
                     DLGKYLEIKGLINKYGKQGCNMTFRIHNEDMIEGFKSLYKIGDVGLLEGTVKSMVVSK
                     SAGFGSRIKKSAFTFLEIEGGDEPLKDGDKIFSDKNYPFTDKNIEAMREKIKEKDKKE
                     KQRDIDKNGKTIEIRDDDVPF"
     gene            30742..31299
                     /locus_tag="PC414_001307"
     CDS             30742..31299
                     /locus_tag="PC414_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HDF2463710.1"
                     /translation="MEVKEKTMNDGFKKVVNEEIKTYISNLYSDRIDGLKESDSQIEI
                     TKALIAFHLDIESIPKDNTNPFFRSKYANLDTILKAVNPLLAKHDLILMQSAEDAGND
                     KVYIKTKLKHSSGEYIESNSAPFKPAKTNDIQARGALETYLRRYAVQSVLALSFEEDD
                     DGNSLTNKSKGKSEEENSTIKKSRV"
     gene            31360..31911
                     /locus_tag="PC414_001308"
     CDS             31360..31911
                     /locus_tag="PC414_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463711.1"
                     /translation="MNCKCRFCKKKLNTNDAYKVEHITSGGNKQNRYYCNEQEYRKEQ
                     QDIYFWKQCQLGIDYIMGYTVINNQKNKMLQEIIKGGYTREELYDCIIEKKDEVIELL
                     NYRKDIEEEYPKLCYVFTILKGSIRDITIKNKQMKYEKENERIYKEVEKSEEYYEIIA
                     PKKVIYNKRQSLFDKVKGVYQDE"
     gene            31952..33478
                     /locus_tag="PC414_001309"
     CDS             31952..33478
                     /locus_tag="PC414_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463712.1"
                     /translation="MVLGTMLKSLTLFSEYKISESDFIIDKVKFFFSLGRIMSKTHNE
                     LDEISVAKFVSSNKLKSEYERYGGWNSLSSAMEYGKETNIAAYIDDLAKNNLLIALDK
                     RGFNVIKEMEHNGLKFIPFEIFQSMKCREVEEFYEGLISSCSVNSIKNNMKVENLLLT
                     KDDREKLKQKTEAGTPYNIIFEYTEKEIGLSNNEESKYIYSLPILSNRTNGIGKGGGI
                     NIIAGFSGIGKTTLLFFNYILAMIYRGEKIVIFANEQKSQYFKSMLVSFIAYNIFNYH
                     DLDRNKIDNGDFTDFEEDLMEKIEKFLRDRCFAENLKFIYMEEFEISEILRKSKELVT
                     HQGFTGIAVDTFKSEDSSDAHYTGKLIENSKLLDSFGNKYNVITMLSMQLLTAQENKS
                     SYLSAGDLSEAKAVKNVCGLLKLMRKVVNELELDSTNKKFFLKPYKLKYNKLKKTTER
                     EYIQFDAKDLQKEYRLLFLNKSRRGQDGDVILLRFYGKTGRLEEIGRCEKVYRGQLSY
                     "
     gene            33488..34549
                     /locus_tag="PC414_001310"
     CDS             33488..34549
                     /locus_tag="PC414_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463713.1"
                     /translation="MEIKDLTNEQVINFMEYLGSDLSPKSNDRQLIFNTCLCHNGDSY
                     KLFYYTESKTFHCYSSCGHIGSLIDLLIHINKYEFKDAINEIKDFFGISNQPMLRKGF
                     RIKKKVEQIHDIKDIQIELLPTPKKPYVYKTFQQVPIEEWENENISFEVLKTYQVYYN
                     LYENQIVIPHFCWHDRARPVGIRVRNLDEDKAKSFGKYIPLWYDNRCYNHRLSLNLYG
                     LNVNKKSIKNSKKVIVFEGEKSVLQMATMYKNNPSVAICGSNFSREQKKILIDLGIEE
                     LIIAFDRQFKVKDDEEYVIWRDKIYKLVQDIKDVVNISVIWDKYNLLGYKDSPSDKGK
                     TIFEKLLKNRINIENFVKQ"
     gene            34654..34926
                     /locus_tag="PC414_001311"
     CDS             34654..34926
                     /locus_tag="PC414_001311"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463714.1"
                     /translation="MIKFLIKNVHMGDKLYCINNIGNYKKGEIYTVSDTSFFGLFHKT
                     KGENCLPIECYFIHLPKKEELESNPKSMTKEEIEDELGYKIKIVSK"
     gene            34947..35798
                     /locus_tag="PC414_001312"
     CDS             34947..35798
                     /locus_tag="PC414_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463715.1"
                     /translation="MSIFNKKYGRNVKITEEEPPSPPPKQPIYNRDNIQYDKEMERKL
                     LDIDRLKIELEKEKKIEEERKKVERVKENEGKEWAWVEGYKCTDKNMKCKEFQYELNK
                     AYSMDGDDINIWNWGFHLCLDLGDVFQYYAPNLSNRFFKVKALVKKADKYKYGKEYGE
                     VTLLSGSVLRGHKINKIAAKEIIFLEELTFDKLKLHIQNSFPEVNTKSKWYAICKLGE
                     QEYYHRMFMNQMKKIRFSDTFSQLLFDECRNYCEILEILNKAIAYSKENLSKDMIIYL
                     LMKDIRK"
     gene            35822..36265
                     /locus_tag="PC414_001313"
     CDS             35822..36265
                     /locus_tag="PC414_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463716.1"
                     /translation="MDKINKIDLIKLKEMNIYDNYQLKKIASKMILHLRYEIMDFCGC
                     GSPEDISFMIKGVLTAIQNKEKNCNLEYTERCYIFEIEFNNVCGIIGSKNDLIQEFIL
                     NALNSYGLLEHGSSIWGSWLSDYGKQILCAFEIVGDCILDVSYLD"
     gene            36292..36951
                     /locus_tag="PC414_001314"
     CDS             36292..36951
                     /locus_tag="PC414_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003401688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HDF2463717.1"
                     /translation="MIKYVMSDLHGMYDKFISMLEQINFNSNDHLYILGDVLDRGDKS
                     LEIIDYIRKYKNITLLKGNHELMFQESYTDVNSSFLWFYNGGKDTFYDLGMKSYEYKE
                     NFYKYIKNLPYLEIIDNFILVHAGLYLPNNYENLSIEQIIELQEEDICVWDRTILNTN
                     KHIKGYTIIIGHTPTQNIKDYEKAEIYKQEGIINIDCGACFDNGKLACLRLDDMKEFY
                     I"
     gene            36962..37489
                     /gene="yyaC"
                     /locus_tag="PC414_001315"
     CDS             36962..37489
                     /gene="yyaC"
                     /locus_tag="PC414_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HDF2463718.1"
                     /translation="MDILKISYNNLNAISKISEVLRDIVNEDTLIVCIGSDRVLADSL
                     APMIGSILEKSTIRNKIFGVLGDSIHALNLEQKIQAIKNNYPNSNIIAIDACISKISD
                     KGTIIISNKPVKPGLGVDKKLLEVGDYSIVGIIGRNKYDIYDTSDPELILDLADVISK
                     SLISILLEKEERMIV"
     gene            37486..37740
                     /locus_tag="PC414_001316"
     CDS             37486..37740
                     /locus_tag="PC414_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463719.1"
                     /translation="MIQEVRQEILLKNGLILYTGDLVEIKYKSDEDVIEHTCKGKIKE
                     AKELFIKLDTSKKYKTSERMIYSWELKEIKKVDDEDEKDN"
     gene            37724..37981
                     /locus_tag="PC414_001317"
     CDS             37724..37981
                     /locus_tag="PC414_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463720.1"
                     /translation="MKKIINSKYRALYLFIFFLIFNLIYSLYFGRNTEIGFYSSTSCI
                     EEYILDIITSIGLFFSMMLAGFDVTENFINSLRDYCEEDAK"
     gene            37992..38201
                     /locus_tag="PC414_001318"
     CDS             37992..38201
                     /locus_tag="PC414_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463721.1"
                     /translation="MELKDYISKVELNDMPLTLDNKYMLNEISISEDSKIFAVKDTSG
                     ITSYYTEIEFNKLFRGINSILENII"
     gene            38276..39115
                     /locus_tag="PC414_001319"
     CDS             38276..39115
                     /locus_tag="PC414_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HDF2463722.1"
                     /translation="MEKELYSFSKLNAFNTCPFQYYLTYVKNLNREQSCYGYYGNELH
                     SLLEQLQQRKITNQEAIQKYNEVIEYANLMDYNFPTPNSRINYLECILHYIENFVPIE
                     CDRYYIEEYFEFDINGITMRGYIDLYYIIGNKIYVIDYKSSSKFSKKDLPKKSMQLIL
                     YAMYLKEKYPDKIIEYVAFDMCKYMNNEKGVLIERNKIDSINDYERAIVKIKYTKELE
                     KQLIDFVVDTVTQIKQLDSNDESVWCKSDDKSNQFFCKTLCSHYNKGCKHKENKSFRK
                     SKS"
     gene            39153..39449
                     /locus_tag="PC414_001320"
                     /pseudo
     CDS             39153..39449
                     /locus_tag="PC414_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895083.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            39466..40047
                     /locus_tag="PC414_001321"
     CDS             39466..40047
                     /locus_tag="PC414_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDF2463723.1"
                     /translation="MDKLKNIFVFLGYSGSGKDSIVTEISKQFNIPILISHTTRPPRN
                     YLEIINKTYYFVDNKFFKKEKNNFIEMRKYIVHDGSTWLYGIHKSELENKKYALTIVD
                     ASGYKALEKYFTGTKTKLVPFFINADEDTIRKRLIDRGDNLKEIERRLVDDKIKFKEF
                     LNSENYIVIANNLSITNAVEQVKIHMKEGIQWS"
     gene            40038..40532
                     /locus_tag="PC414_001322"
     CDS             40038..40532
                     /locus_tag="PC414_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HDF2463724.1"
                     /translation="MVLALDVSMSSTGWAVINRNKNILKYGKIVTKKDKFKSEDERMC
                     YICNAIQELIITHNIQIVLIEDQFTSRNSKTILSLRKLLGAIMRTVKLNNIEIEYMYP
                     VSIRKYLLNNGKAKKEEVAAYVRENIIDIGEYIDRTCKAKTSDIYDAIALGIAYLNKL
                     DTNR"
     gene            40556..41059
                     /locus_tag="PC414_001323"
     CDS             40556..41059
                     /locus_tag="PC414_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyuridine 5'-triphosphate
                     nucleotidohydrolase"
                     /protein_id="HDF2463725.1"
                     /translation="MSINVKIKKLNQNAIVPSYAKEGDSGIDLYTLKDTVIPAKSAVA
                     ISTGISLEIPYGYEMQVRPRSGISLKGMFFTRYVTIYDKVQPKKQIRKIFVAHPTVRL
                     GTVDSGYRGEIKIITYNEEDFDILIPKHTKLAQGVFQQVPRAILKVVDELTSSERGSN
                     GFGSTGI"
     gene            41060..41200
                     /locus_tag="PC414_001324"
     CDS             41060..41200
                     /locus_tag="PC414_001324"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463726.1"
                     /translation="MNKYIILEKYNYYYDDKCMTLYHLENIYTGLRFSIEKIELEYYN
                     IS"
     gene            41224..41397
                     /locus_tag="PC414_001325"
     CDS             41224..41397
                     /locus_tag="PC414_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463727.1"
                     /translation="MYRYDDYVNLINDLELPKEIRNKCVRVIMTLENMQDIMVVHKFK
                     TYVVNEKYIEKVA"
     gene            41547..42266
                     /locus_tag="PC414_001326"
     CDS             41547..42266
                     /locus_tag="PC414_001326"
                     /inference="COORDINATES: protein motif:HMM:NF019976.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009617778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HDF2463728.1"
                     /translation="MSEEVSKTVKEVNNIIKDTKIQVKGGFGELRLDFILSRLGKDYI
                     IIKDIIVPGSNKTTQIDSIVISMYGIFVIECKNFSGYIYGNDKDKVWTQIVGKTKNTF
                     YNPVRQNYAHIKAIENIIGNKYNIYSIIVFSDKATLKNVMISGNNIIVINESEILSTI
                     SKCKDVTIDKEEIKIIRDKIFDCMKTTNQNTREHVRNLKNITEEDKCPRCQIGHLIKR
                     KGQYGEFWGCSQYPKCKYTRK"
     gene            42555..43667
                     /locus_tag="PC414_001327"
     CDS             42555..43667
                     /locus_tag="PC414_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDF2463729.1"
                     /translation="MNAFIRKRNKNYVVYLEFRDDESGKRKQKNMGAFDKKRDANKRL
                     AEVKDSIYKDSFLVPNEITLAGFILDFLEKYKDNISASTYKSYIAICKNHINPSIGKY
                     RLQELRNIHIQNYIDDLAGNLNPQTIKVHINVLRLAIKRAYRIKLIKENIIDGIESPR
                     IKKFKNEIYDKEQMLKLLELAKGTNMELPISLAIGLGLRLSEVLGLTWDNIDFDENTI
                     TVNKITSRLDGSVILKEPKTESSVRKIFAPIELMNLLKNYRLEQNKKLLRSIVRNEYN
                     LLFFDRKGNPIAEDVMSKKFRKFLENNDLPHIRFHDLRHSHVTLLINSKVPIKVISER
                     VGHSNINTTLNVYSHVLKEMDKEASDRISENLFKAN"
     gene            complement(44165..44359)
                     /locus_tag="PC414_001328"
     CDS             complement(44165..44359)
                     /locus_tag="PC414_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463730.1"
                     /translation="MPELEINISISIPIFLVISYLAIVSIVMLGISALNNFTNITSVP
                     INALGSTVGFVIFQLLVSFF"
BASE COUNT        19344 a         3937 c         7631 g        13989 t
ORIGIN      
        1 aaaagtatat gtcgaaaaca tataatatta aataaatata tactttaagg agtgatttaa
       61 tgattataac tatttgcgaa aatgatgata tgcaaagaga tatcatctat gattttgtta
      121 agatattttt aactaaaatc aaggtaaaac attatgacat tttaatgttt aagaatgggg
      181 aagaactgtt agaagaatat ccaaacaaag tggatttaat tttaatggat ataaatttag
      241 gaggtagaaa tggatttgat atttcaaaag atattagaaa atttgataaa gatgcaagaa
      301 taatttttgt aactgtatca gagaaatatg ctatcaaatg ttatgagata aatgcattta
      361 gatatatgat aaaaccgata tattataaag aatttgaaaa atatatgctt gactttgcaa
      421 ttgattggag aaaaagagaa gctattatta ctataaaaag aaaaaaacaa ataataaaaa
      481 tagaggaaca taatatttta tttgttgaaa tagtgaatag aaaattaaga gttgttacta
      541 aaaatgaaga acatataata aatgataaaa tggatatgtt tgaagaaaag ttaaaagagg
      601 atatattttt tagatgccat aagagctatc ttataaattt acttgaggta actaatttca
      661 attataattt cgtttattta acaagtgaga aaataaaaat taaagtagct cctaaaaaga
      721 ccaaaaaact taaagagaaa ttagcaaatt tttttaggga aataatatgg taaattaaag
      781 aaaaatttct ataatcgaat atatgttctt gataaaagta tgtgtacaag atataatatt
      841 attagtagga aaataggaaa aatattaata tttttgaaca ttttgacatt aattgtgatt
      901 ttagtgttga atttctgtta aaaaaatctt attatttaat catagaaatc tactgttata
      961 gagtagaaga ttgttaatta taaatgattt taaattatct gagataaaat tttttgtttc
     1021 tgaaaagaac gaatgttcga atgaggtgta gagtgagagg atgtttatta aaagatatta
     1081 gattatctta cttaattcat aatgcatata tgggggttct ataataatga aaaaacaagt
     1141 cacaaaagaa atacttaaaa tgctagaaaa aatggattta tcttatttac tcataattaa
     1201 aagttttgta tctaaattaa aatagccctt attgggctat ttactaagtt tatcaattac
     1261 tttctgtatg acttctcttt catcatcagt aagttcagca aatgttaaga atactttttt
     1321 taaaaattca tcattacttg agaatgtttt acccatcatt gctgctaatt caatatcatc
     1381 tatattttta aacatttctc catttccatt tcgtaaccag tcctcattta cattaaaaac
     1441 ttcacatatc tgaaatataa atctatctgt tagctttctt tctccttttt caagtctaga
     1501 aatagttgca tcacttacta ataatttttc tgcaaattct ttttgtgaga gacctaattt
     1561 aattcttaat tcctttaaat tctcattcat atttacacct cctattatat agtatattaa
     1621 taaaaattga caatgtcaat aaaataggaa tgataaattg caaaaagcaa aaaaaataat
     1681 tgacaaatac agaatgtggt actacaatgt aaatatacaa aacgcaatta ataatttgac
     1741 aaacgcaaat aaataaggag gtagattatt tgtataataa taatgataaa aaattaataa
     1801 atttatttga atcatttaaa aaaatagact atacagaaca aagtttagtg tattttttat
     1861 ctttaagctt tatagtaaac agaaaatgtc ataaagatgc aaatttgtat agtaggagag
     1921 gtgaatgtaa ttatgaataa tttaaatatc aaaggaaaac aaaaatttat ggatttagaa
     1981 atacctatta tagaaggagg ttttggagag ggtaaaaaat gtataacaga taaaactata
     2041 tcagaaatac acaatatgga gataaaacat gttagagaat taataaatag gaacataaga
     2101 agatttataa atgctattga ttttatagat ttaaaagtgg tcgttgataa cgactacaat
     2161 ttagaagtat tggagttgct tagttataga aaaatgcaaa tatcaaaagc aaataatatt
     2221 tatcttttat ctgaaagagg atattcaaaa ttaattaaga taatggatac tgatttagct
     2281 tgggagatac acgataaaat aatggatgaa tacttcaata tgagagaaca actcatagat
     2341 agttatatga tacaagattc tattgaaaga gctaaaagat ggatagaaga agaaaaagaa
     2401 agattattac ttaaagaaac aattcaagtt caacaaccta aagtagaata tcatgataaa
     2461 gtattaaatc ctgacaaatt agttacgaca acagatattg cgaaagattt aggaatgaca
     2521 gcacaagcat taaataaatt actacattta aacagaatta tatatccaga aagaataaat
     2581 ggtcgaataa aatgttggaa accatataaa aattatttat ggttgatacc tgaatatgca
     2641 gattttaaaa taacagaaca tgatacgcag cttaaatgga ctgagatagg tagaaaatgg
     2701 ttaatagaat ttgtagagaa cattaaaaaa ataagttaaa tcaagaagtt aaaatgtacc
     2761 ttgaaaagta gatatttaaa aatataaaag ataggtgatt aaatgataaa acaaatacta
     2821 aataaaagag gtagtctatt aaatctagca agtgagtatt tgacagaaca agactacctc
     2881 aagtttcaag gatggtatac acatagatat agtaagctgg atgcttatga tgcttctata
     2941 aatgaatatt taaattataa aaacgacaaa aaattaataa cttatgtatt taatttaaaa
     3001 tttcaagaag ctctcataga aatataatat tactaaacat ggtgattgaa atgaattata
     3061 attatgtaac taaaattgac ttgaaatatg gaaatacaca atttgggttt gagaaatata
     3121 gcgatgcttt agataagtac agagaactta aagaagaatt taagtatgtt ccatgtatta
     3181 taaaacttca ttcatatgta gcgcaccaag atttatatgt gaaagaaatt aaagctgatg
     3241 gttttgaaaa gaaatattta gatattaaaa atttaataaa attgtttaaa gaacataaac
     3301 aaaatatatc agatgaacta agtctttgtg ataagaaaaa ggaagaagta ttacatgtaa
     3361 tagaagaaat gaatttagac acagcttctt taaatgataa gattgatacc attaaagaat
     3421 taaaaaacac tttagttaag agaaggttgg ttaaatatga actaaataaa tactttgcct
     3481 tttgtaatac attatatgaa attgaagaag agttaaaaaa atatgaatct aaatataaac
     3541 aaaaagaaaa tcaaggaaaa agcagattca aaaaaaataa aaaataggtg attagatgga
     3601 agaatatcat tttaacttaa taaaaaagct ttgtgctgaa agaaaaatac acaaaggttg
     3661 gtttagaaat attgaaaaat gtggttgtgg aggtaaatgt aataagaaaa attgtaagat
     3721 gttagaaaaa ttataaggag atacatatgg aaaatcaaat tgaattaaca gaaaaagata
     3781 tagagaacac tttaagtgct ttaaatatta ttaaatatat ttgtataaaa tctaaacatt
     3841 gcaaaaattg tcctcttgga tatatgaaaa atcttgatga acataattgt atgctatatg
     3901 aattaataga caagggttta cttccatgtg attggtctgt aaaaaaagtt acaagattgt
     3961 tagatacgac agaagaataa aacaaaggag ctggttaagt ggagttagat ttcagaattt
     4021 ggaatggatt tagaatgata catggagaag aattaagatt actaacagaa gagatgttta
     4081 taaaaagtgg atattctgaa caatactata acgatatttg tataccaaga gtagaagatt
     4141 tgaaaataat gcaatacata gggctaaaaa attgttttga aaaaaagata tttcaatatg
     4201 atattgttaa atttatcttt gatggtgttt ggaaagtatg tttagtaaat ttatctttgc
     4261 caaaaggagc aacagttaca tttgaaagca taggtggaaa atatactttt aatttaagtg
     4321 aaatattttc aaaagatagg aaattagaaa tcataggtaa tttttatgaa aataaagatg
     4381 taatcaaaaa atataatctt agtattgaat aggagaagaa aatggatgaa atgataatta
     4441 gggtgaaaat gggagaacca ttaccaacac taggaaagat aatcaaatgt actacatgtt
     4501 taactaatga agagaaatgt tttaaggtta ataagatatg taaattaagt tggattaacg
     4561 ataggaatag tgaagggtta attgtagaat gcaaaggtaa atatattaaa tattaataag
     4621 aataatgaag ttaaaatcaa gattttaata gaaaaggagt gattaaaaat gaagaaaaat
     4681 ccaataaaaa ttaaaaaatg tgactgtggt ggagatatat atgaacttaa tacacaatta
     4741 ggcactatgt atcaatgtaa aagttgtggc gagatgtata gatagtgctg aaatattagc
     4801 taaagaagat gaggtgaagt caatgagtaa aaaagataag ttatctattt ttataagttt
     4861 aatattaaga cataaaccag aaactattgg aattaaatta gatgactatg gatatgcaga
     4921 tgttaatgaa ttgattgaaa aaataaataa tactggtaga aatattaata tagaaatcct
     4981 tgagcaaata gttaaagaag ataataagca gaggtacagt tttaatgatg atagaagcaa
     5041 gattagagca aatcaaggac attcaattaa tgttgaggtt gaattgaaag aattagaacc
     5101 gccaaaatgt ctttatcatg gaacggctac tagattttta gagaatatta aaaaggaagg
     5161 tattattaga caaagtagac tgtatgtaca cttatcaaat gatatagata cggctgttaa
     5221 ggttgggaaa aggcatggtg tacctgtagt tttaaaaatt aatacaggaa aaatgtatga
     5281 aaatggttat aaattttatt tatctgaaaa taatatttgg ttatgtaagt atataccatt
     5341 tgaatatgta gagaaaataa gtagagtagt taaataaaat tatagaaaat aggaggttat
     5401 tgaatggatt tcaattttag aaagatttgt gaaaaagatg aacttactga tgattttatt
     5461 ataaaaattt gccagttgtc acatgaggag tttggaggca ctatttatgt accatgtaat
     5521 attcatccat gtgggtggga tgagttggaa gttccgaatc caaaggattt agattcagat
     5581 aaattaatgg aagaattttt attagaatat aaatttgaat gtgaaaatat ttcgagcaat
     5641 atttatgata gtgaccatcc atttataggc aaaacattga gagagattct tatgatagtg
     5701 gattgtagat tcataattgg tgttatataa ggagggtatt aaatatggaa aatatagaat
     5761 taacacctag agattatttt gaggatataa aaaataaaaa gcaaaaaaat acaaatgaaa
     5821 atcttgatat agtatatgaa aactctttga ttcttttaaa caaatatatt gtaactggtc
     5881 aaaaaaaggg tgctaagaaa ttaatgtttt tacttgaatg tattgaaaaa gaaagaaatg
     5941 taatagagct gggaatagat acatttgtgt ataaagaaga tatagaaaaa tatattgatg
     6001 atatatctaa aaatacagta aaaataatag aacttgaaaa ttatgaaaga gaaataccag
     6061 atgaaatagt agaaataata gaaaaaacaa aagatatatt tgataaaatg tatgttttgt
     6121 ttactgatta tacaggtaaa attacaaagg agataagcaa agagagaaga gataaagacc
     6181 caatattatt tggtgttttt tcagatgaga aaagcagagc tattgtagat agattttatt
     6241 atcttggaga ttgggaggat gagtattgtg atttaacatt agagaaaatg ataaatgatt
     6301 ataagaaaaa aggaagacaa gacatattaa aacatataat tacacctaaa tcattagatg
     6361 agttaaaaaa agaattagat tctttagaaa aaaataatga tacatataag caagttgcca
     6421 atataaagcc attacctact acaaaaaact ctagtgtaga accacctaaa ttaaatatat
     6481 ttaaaaaaat aaaaaataga gtgtttaaaa atgaaaagta atgttgattt aacagagaat
     6541 agggatttta gagatgtaag aggaagaggc atcaatattt taggtatcga agatgcgcca
     6601 aatagaatgt tgagttttag tgattttgga attggacgtc tttatggtaa aaatttagaa
     6661 tttgaattta ataaaaatga agtattttta actggaaatg ctaatgacag agaagaaaaa
     6721 aagtttctta tcctttgtga agaaaataag gtttgtgtta gatgtggtga tgaattaaga
     6781 gaggggtggg ataaagtaag catagaacta tgtaatgaat gttatgaaat gttagatgtt
     6841 gagtataaaa ataaaaagat aattagagaa ttatttttta gagatagaga tgtaaatata
     6901 gagttacttt aaaaaatata taagaaaaat aagtagagga ggaatttata tgaaagatgt
     6961 ttgattatat agctgggatg atgagtgctt tggaagtgat gaatacgaaa gtagagagga
     7021 agctattcaa ggagctaata aaactagtat ttttagtgat gacaacagta tgacaagaat
     7081 aatgagaata taaaggagtg atgtttatta aaagttttag atgtatattc agacataagt
     7141 tttcaaggat aaaagattgt caattaatta caacaaggag tcagcaatat acctgtaaaa
     7201 tttggatgtg taaatattgt ggacaaattg tagctggttc aactcctgtt tatcatccta
     7261 atatgaaagt caatgagtat tgtgtaaagt gtgagttttc ggcatgtgca aataattatg
     7321 agggatattg taactctaaa gataaagaaa ttgaatttaa gtctatttgc ttaaaaggta
     7381 ataaagagat tttgcagtgt aaaaattttg atttggaata aaatttagct taagtaggag
     7441 aattttagaa tatgaaaata aatgtacatg ttaaaactaa taaaattggt tcagactgtg
     7501 agctggagtt tgaaatagat gatgatgatt taaaagatat gtctgatgaa gagagagagg
     7561 gttatataga taaaattgca tgggattatg tttttgaaaa ttttatagat tggaattggt
     7621 atgaggttga agattaagtt aaaacatttg ttttaatgtg attttaccta gtagatacaa
     7681 ggaggttttt aatgaaagag cttttagaag taaaaaaaat atttgatagt ttagcatcta
     7741 catctagtag aaaagaaaaa gagagaatat tagaaaaata taaaaataat agaatgtttg
     7801 ttgaatgttt acaatttcta ctagattcaa atattctcac tggaatatct aagaataaaa
     7861 tatgtaagaa tttaaataat actggtcata atgaattaga gaacatatat gatatgcttg
     7921 actatttaat taagaataat actggcagaa atatagatgt aaaaacaata caagtatttg
     7981 ccagtaaaga tgaaaaatta aaagatttta tttttaattt agctacaaaa tcaattaaat
     8041 taggaataac atataagact gtagacaaaa taatgccagg tttaattatc taataaattt
     8101 tgtatttttt acaaaaaact attgagaaca tagaggttat atgatataat aaaaatatat
     8161 tgagaacaga gaagttaaaa aggggggcaa tgagatgact gacaaaataa taatagcaag
     8221 tgtagatgga gaggaaatga tatttagaac tgaaatgaaa aagttagaaa aaggagatag
     8281 tcttatagtt tctgtaaatg gtgtaaaaag attagctcta ttcattaggt atataagtac
     8341 ttgtgaagaa tatatagaag atatagcatt tcaaaaagta gatgaaatat tatatgaaaa
     8401 cacaattgcc aaagaaaaag aaagagttaa aaaggtattt aaagataagt gtatgaaaaa
     8461 ttataaacct attgttaata cagaaataga ctcttatttg agagaatgtg gtttcaattg
     8521 tgatggtaga caatttttca aaagtataga caatacacaa attttaatta gcagggattg
     8581 tgataaagat gttttgactg tatcgaaagg tttgacaata actaaaacag atataacaaa
     8641 taaaaattta attacagtta ttaaaataat aaataatagt ctattaatag ttaggtaata
     8701 gaaataaata ttgaactttt aataagaaaa aagattttaa ataaaaggag tgatttaatg
     8761 aagaatgtta ttaaaagaga tgggaggata gttccctttg atgaaaagaa aataataaat
     8821 gcaatagaaa aaagcttttt atcattaagg aacaaaggaa aaagtgaagt atttgataga
     8881 gaattagcaa cactaatagc taaaaaaata aaaaataaac catgtgaaaa aatgcatata
     8941 gaagatatac aagatataat cgaatttagc ttaatggcta gtaaaaagaa agatgtcgct
     9001 aaagaatata taacatacag agagaaaaga aatatagcaa gaggaagaaa aacttataat
     9061 gattatatgt ctattgtaaa tactgaaagt aatgatataa ctaaagaaaa tgcaaatatg
     9121 aacgcagata ctccagcagg tatgatgatg aagtttgcaa gtgaagctac taaaactttt
     9181 actgaccatg tacttttatc tgaagaagca aaagaagcaa tgatggataa ttatatacat
     9241 atacatgata aagactatta tcctacgaaa tcattgactt gtatccaaca tcctttagat
     9301 aaaattttta ataatggttt tagagcggga catggctcta gtagacctac taaaagaata
     9361 gagacttcaa gtatattagg atgtattagt ttagagactg tacaaaatga aatgcatgga
     9421 ggacaggcga taccagcttt tgatttttat aatgctccga gtgtaagaaa aacatttata
     9481 gaagaaataa aaaatataga aataaaggat gatattgatt tatctcattt atacaatata
     9541 gaaatagagg attatataat taaggactta aaagaattag acagcgaaaa aagaaatgtt
     9601 caacatgcta taaacagaac tgtatggaga gttcatcaat caatggagtc atttatacat
     9661 aatatgaaca caattcattc aaggggtgga aatcaggtcg tgtttagttc tataaattat
     9721 ggtacagata cttcagcaga aggtagatgt ataataagag aaatactaaa atctacttat
     9781 gaaggagtcg gaaatggaga aacaccaatt ttcccaattc aaatctggaa agtgaaagaa
     9841 ggtgtgaact ataatcctac tgatagaaat tatgacttat atgaattagc atgtaaagta
     9901 tcagctaaaa gattttttcc taatttttta aatttagatg caaccttcaa taagcatgta
     9961 aaatggaaaa tgtgcgaccc aaacagatat atttatgaag tagcaacaat ggggtgtcgt
    10021 acaagggtat ttgaagattt gcatggagaa aaaacaagta taggaagagg aaatctatct
    10081 tttacgactc ttaatttagt aaaaatggca ttagaggcta aagaaaaata tccaaataat
    10141 gaagctaata gagttgcaga gtttattttt cttcttgata aatattcaga tgtagtagca
    10201 agacaattat atgatagata cttatttcaa tgtactgcta aagcaaaaca gtttccacta
    10261 ttaatgagtg ggttatggaa aaactcagaa aatttaaatc caaatgatga tgtaagcgaa
    10321 gttttaaagc atggaacact tggggtagga tttataggtc tagcagaatg tttaatagct
    10381 cttataggtg agcatcatgg tgaaagcgaa aaggcacaaa aattaggttt agaaatcata
    10441 tcacttctaa agtctaaagt agaaaaatat aaaaagaaat ataacctcaa ttattctgta
    10501 ttaggcacac ccgcagaagg tctttctgga aaatttactc aaaaagataa aaaaatattt
    10561 ggagaaataa aaaatattac agacaaagat tattatacta atagttctca tgttccagtt
    10621 tggtataact gcaatgttga acataaagcg aaaatagaag caccttacca tgaattagaa
    10681 ggtggtggcc acatatttta tgttgaatta gatggggatg caacacataa tcctaaagct
    10741 ataatgaaag ttgtggattt aatgaaaaag tacaatatgg gatatggttc tgtaaatcat
    10801 acaagaagta gatgtatgaa ttgtggtttt gaaaacgcag attcagagtt aaaagaatgt
    10861 cctaaatgtg gcagcaatag tataaatatt atccaaagaa ttacaggtta tttagttgga
    10921 actacggata gctggaataa tgcaaaatta gctgagttaa aggatagagt cacacatgga
    10981 taaagactat atttatattt tagatataaa acatgacaca attgttgatg gagaagggtt
    11041 tagaacatct atttattgtt ctggatgtaa tcatatgtgt agaggctgcc acaatcctca
    11101 atcttgggat ataaaaaatg gaactttaac taaagtatta gatatttata aggaaatcat
    11161 ttcaaataaa ttttcagatg taacattttc aggaggagac ccacttctac aactgcaagg
    11221 atttgttcat ttagctaaat taattaaaaa gaatacaaac aaaacaatat ggtgttatac
    11281 aggttttaag tttgaggaac tcataacaga caatgaaaag ctagaattgt taaaattaat
    11341 agatgttctt gtggatggaa aatttgaaga agataaaaaa tctttagagt tgatttttaa
    11401 aggttcgtca aatcaaagga taattgatgt tcaaaaaaca ttgtatgaaa aacaaatagt
    11461 attatatgaa taagagaggc ttagccctct ctttggaggt aaatgttatg accaaagaag
    11521 aatttattac taaatttctt aaagagcagc agagaagaat aaaggtttta tattatgaag
    11581 ttgaaaattt caagatggat agaaggttta atgagaggaa aaagtctagg agacattaaa
    11641 atgtttcttt taatataagg aggaagtagc tatatgtatt ttgaaacgga cattataaaa
    11701 aataagaata ctgtaaataa aataataaaa tattgtggtg gaaatactga aaaattgaaa
    11761 gattggctaa aagaaataga aaataaaagt atcagtagtt tagaagaatt aaaagaatat
    11821 ccattaaaaa gaaattataa atttgtattt tccaaagaag aagctcctaa tttagataag
    11881 cttataaaaa tgaaactaag agataattgt ttagaaaaat gtgagatttg tagtttagaa
    11941 aatctcttaa gtatgctaaa tgatgagatt ttagatttaa ataaagaaga tatctcttat
    12001 gaagataata ttgaatggat tgaaaattgt aaagaagaac taattaaatt aaaggtagga
    12061 agtatttgca tagattggta gaaaaataaa ggagtgatat tgtgagattt tggtgtgaga
    12121 attgtaataa atacttcaat gcggaagaaa cattacagga atataattat tttttaaatg
    12181 aagagatact tatatgccct agttgcaaaa aagatttaat acctatagct agggaaacag
    12241 aattatcatt aggttttgat agtgatacaa accaattaac ttatgttgaa tatgattcta
    12301 gtgattatag tttacttaga aaggtaaatg cagatgttga agatgtagta aaacctataa
    12361 tacattacat aagaaattta aataaaaata gtttagattt aaatggaatt acagaatagt
    12421 gcggaaagcc cactccttta ggtgtgggat ggatagcaca aacagtgtat aaaaagtatt
    12481 gacacataca cgtataatgt taatactttg tattctgccc tagatataaa agaaaaatat
    12541 ttaatataga aggtttagaa gatagattta aaacattagt taaaaataag tgtaaggaaa
    12601 tggatatcga aattgttgca atagaatgcg ataaagacca tacacatatg tttttaaata
    12661 gtcttccgac tctaaatcct tctgatataa tgcaaaatat aaaaggctat acaagtaaaa
    12721 tattaagaga agaatttatt gaattatcaa aaatgcctag tttatggact agaagctatt
    12781 ttgtatctac agcaggaaac gtatgtagtg aaactattaa aaaatatgtg aaaaaccaga
    12841 aaacaagata ctagaaagtg aggtgaacac tatgtcaaat tatatattaa cattgcaatt
    12901 aaaaacagaa aagtttcaag aagacattat atgtaatgct tttgataagt atagaaaagt
    12961 atataattca tgtttatcag agctatttaa aagatataac cacatgatag aatctaaaga
    13021 acaccaagca aattgtaaat ataagggtaa agatagaaat aaaatattta gtgaaattaa
    13081 taagaagtat aatttaacag agtattcact tcataagttt gttaaattta ttggtaaata
    13141 ttataaacta cattctgcta taactcagaa gatagctaca agatgtttta atgcttttaa
    13201 taagtatatc tatcatcaag ctaaacgagt taattatatt agatataatg aattaacatc
    13261 aatagaaggg aaacaaaatt caactggtat tagttataaa gatggtatta ttaaatttaa
    13321 taagatgact attccagtaa taataaagaa taatgacaat tatgctcaaa gagcgataca
    13381 agataaaata aaatattgta gaatacttaa aaaagaaatt aagggtaaaa ttaaatggta
    13441 tgtacaatta gtcttagaag gaattccacc taaaaaaaca accaatcaag gaaaaataaa
    13501 aggacaaatt ggtttaggta atgtaggtat agatattgga actcaaacta tagctatatc
    13561 tagtaaatgt gatgttaagt tattggagtt agcccctaat gttaataata tagataggca
    13621 aataaagtta attcaacgta aaatggatag aagtaaacgt gcaactaatc ttaataaatt
    13681 caatgataat ggtactataa aacaaggaaa tagagataag tggatattta gtaatcacta
    13741 tcttaaatta aaaagactaa gaaaagagtt atatagaaaa caagctgaaa taagaaaaca
    13801 agaccactat atattaatta acaaattact aaatttaggc aataagtttt atgttgaaac
    13861 gatgagttat aaaagacttc aaactagagt caaggatact actattaatg aaaaaactgg
    13921 tagaattaat aaaaagaaac gttttggaaa atcattagca aataaagcac catcaatgtt
    13981 tttgactatg ttagacaata agttaaaata taataatgaa agattatata agatagatac
    14041 tgctaaatgt agagccagtc aatataatca cttttcagat gagtacaata agaaggaatt
    14101 aaaagataga tggaataatg gtatggacat tcaaagagat tgctattctg catttttaat
    14161 aatgaatgtt aatgaagatt taaaatcaat caacagagaa ttgtgtgtta aaacatatga
    14221 taactttaaa atattacatg ataaagagat aaatagatta aaaaaattaa aattaaatgg
    14281 ttataaatta atatctagta tggggatata aaaactatat aggttgagaa acgagcctaa
    14341 tattaatgtt aatggtgtca acagatactt tgttaataaa agtcttaggg aatatgatta
    14401 gtgataatat gttgtaggtt atgatttatt ataactgaga gtattgtaga aattcatgta
    14461 gtacctaaga accccacgcc tttaggcgta ggagtttcag aataacaatg aatggtaata
    14521 tataagaata aaaagaagga tattataaga gatgagtcga tgtatgatta tgggatgtct
    14581 ttgtcatcta ttatgaaggt tatcttaaat catcaagaag atatagaaaa gttaaaagaa
    14641 gaggaggtag cagaatttat aaaaaataaa ttagaaaatg agcatgattg tgatgaatta
    14701 aaaataataa gtgaggtatt taatgaaata tattaaacat gtagataatg gagtaatgag
    14761 agcaataggt attgataatt ataaaccttt attaaacatc ataaaagaaa aagtgaattt
    14821 agaatctaat taaaacagtt ttaattagcg aggtgataca tatgggatta gatttaggtt
    14881 tttattcagt aaaaacaaaa gttacagatt tagatattta tttaaataaa acaagaggtg
    14941 agaaatataa ggaattgaat aatgatgaag aagagtttta taagtttgaa gaattattag
    15001 atattgatat atctttagaa gattggtcag ttattcgtat gatatataaa gccaaatggg
    15061 aaaaaggtta tgaaagttat gaagatgaag ccatacttat tacaaaggaa gatttggaag
    15121 attatataat tccttttctt aaacaaaaag atataaattc taagatatac aataaggata
    15181 aagagtatta ttataaaaat gataaaaaag taacagttga tgataggtat acacaggagt
    15241 tttgggattt agtattaaaa gaatttcaaa aaatgttaaa cataattgat tttgaaaaac
    15301 atagtttaat tattatgtat tggtattaag ataataattt taatttattt ggaggtttat
    15361 aataatgaaa aaaatgtaca tagcaattaa ttcagactta aatatgagtc caggcaaagt
    15421 tggagcacaa gttgcacatg cagtttatga ttatttgtat aacaaaattt tagacactgt
    15481 ttcttgctct tatgaaacag aaataatagc acatgaatta atagatttaa aatgtgattt
    15541 aacgtcattt aagaataatg gggatacaat atgtatatta aaagctaaag aggcacaatt
    15601 attaaaattt aaagaaaaag gttatttaac tatagtggat agaggattaa cagaaattcc
    15661 taaaaatagc ataacatgtg ttaacttagg aatatttaat gaggatgaaa taccatctac
    15721 aattaaaaga ttgagattat attaaggaga aagacaataa atgtattcta aaggtgatgt
    15781 agtaaaagta tggtgttatc aatgtgaaag gatagaattt caagaatttg taggatattt
    15841 taattcttat tttgataatt ataaattaaa gaaacgatgg aaatgctcta agtgtgatgt
    15901 catcaataat ttttaagagt ttaattaatg gaggaatgag ttatgaaaat aaagaaaata
    15961 attttaggat tagataactt agagaatgta gagttgggta ttggagatgt aggaggtttt
    16021 tatttagata atatacatga aattattgga ggatttgctt tgagttatat aggtaaatgc
    16081 aagaaggcag attatttaca tatacaaata cataaacgtg ttgatgaaat tcaaagattg
    16141 catgattata aggatattac aaatgtaaag atagaatacg aagacggaag tgtggaggat
    16201 atatatgtac cttataaaca agaaggggat ttcttgggtt tagataatct ttatcaaact
    16261 tcttttatta gtaaacaagg ttatttacat atagttatct ctaaggataa aacagtagaa
    16321 gaagtcttca atgaagaatt tgaggatgat gaaggggtag aatttagttt taatatgtat
    16381 ggaatataaa acttaaaatg gaaggagatg agcaaatata attaactctt ttaagaaatt
    16441 aataaagtca aaatatataa aaaataacga atcaataaaa ccattagaaa gaatttttga
    16501 atatgaaata agaatcaaag aaaaaagaac atattttaat ttagaattat tgatttatga
    16561 aaaaagaatt aagataatta aaaaaatatt tttaaaagat atactatgcc attatcgaag
    16621 agatatagaa caactcgtca tttatgacct ggataaatat cttaatatga cagataaaga
    16681 aatggaggaa tatattataa atttaataaa agataaaata aaagaagagt atggggagaa
    16741 tatacttcct gaaatactaa tggaaaaatt tataaaactc gaaagaaaag gttcggtgac
    16801 tgtagacgta cgggatttaa tagagggata ataaataaca agttaaaata caggttttaa
    16861 aaggagaata acaaatggaa gttcaaggga aatataacaa agctaaaata tttacagaga
    16921 atgtagattc gataacaata ggacaaataa taaatttatg taatcaaaag gaatttaaag
    16981 aatccagtat aagaattatg cctgattgtc atgctggtaa aggatgtaca ataggaacaa
    17041 caatgactat aagtgataaa gtagtaccaa atctggtagg ggtggatata ggttgtggaa
    17101 tggctactat tccattagaa aaatatatta ataatataaa ttatacaaaa ttagataaga
    17161 ttataagaga ttatattcct catggattta aaatacataa taagtcaaga gaaaaactat
    17221 taaagaattt atttaatata gacataaata atcttagatg cgaggtgaat aaaaatagag
    17281 catatatgtc gttaggaact ttaggtggag gcaatcactt tatagaagtt gataaaagtg
    17341 aaaaaggtca aatgtatctt actgttcata caggttcaag aaatttagga aaacaaatag
    17401 ctgattatta ccaagacaaa gctataaaat attgtattga aaaatataaa aagtcatatg
    17461 atgatgcaaa agagttttta atatctactc taaaaaaaga caataaagaa catttgataa
    17521 ataaatattt gaaagacctt agtgatagaa aattgaataa gccacatgat gatttatgtt
    17581 atttagaaaa tgatttaatg gaagattatt tacatgatat gagtatagct caaaaatacg
    17641 ctgctgcaaa tagaatggtt ataattgcgg atatattgtt taaatataac agcctagaag
    17701 taaattatga ccattttata agagatatta aatgtgatgt tatagaatgt attcataact
    17761 atatagatat ggatagtaaa atacttagaa aaggagcaat ttctgcaaat aaagatgaaa
    17821 cagtaataat tccagtaaac atgagagatg ggattatatt aggaaaaggt aaaggtaatt
    17881 ctgagtggaa ttattcagca ccacatggag caggaagaat tttatcaaga ggaaaagcta
    17941 aagaaaaaat atctttagat gaatttgaag agtctatgaa agaaatattt acaacttgtg
    18001 taggtcaatc aacactagat gaagcaccac aagcatacaa acctatagag gatatcttaa
    18061 acaatatagg agatactatt gagataatgg aggtgttaaa gccaatttat aattttaaaa
    18121 gcaattagaa tcttgttatt taaataaaat caaaaggaga aaaaagggta tgataaaatt
    18181 aataacacat aatgatttag atggtgtaag ttgctgtatt atagcaaagt atattttacg
    18241 agaacaaata gatgttaact attgtaatta taaaaatata aatgatgtag tattagatac
    18301 attaaaaaat cacgataaat atgaacaaat attaataaca gatatatcag taaatgaaga
    18361 tgttgccaag aagttagata ggattaaaaa taaagttcaa ttgcttgacc atcatccaac
    18421 agcactattt ttaaataatt atgattgggc taatgtagaa atagagtgta ataaaggcaa
    18481 aacatgtggg actcaaatgt tatttaatta tttacaaaga tactatgatg atacttcaga
    18541 atctacatta aaatatttta tagaacttgt tagaaggtat gatacatggg aatggaaaga
    18601 taaatataat gatttagatg ccaaatattt aaatgattta atgtatatta tgggaataga
    18661 ggattttata atggacatgt taactaaaat agatagtgat gagatgtgtc tatttgataa
    18721 attaagttta aaattattag aaataaaaca aaaagaaata gatagatatg tagagaaaaa
    18781 agatgaaaat ataatggtta aagaaattct aggatataat gcaggaattg tatttgcaga
    18841 aaatcatata agcgaattag gaaataaatt aagtgagtta catccagaac ttgattttat
    18901 agttataatt aatcgagaaa tcatttcata tagaactata aaagataata taaatttaag
    18961 tgaaatcgct aaagtatttg gaggtggagg aaatccgaaa gcatcaggaa gtcagattga
    19021 tgagagttgt atagataatt acataaaaga tatatttaat ataaaatctt aattttaaat
    19081 ggaggtatat atgaaatttg aaaacaacat gattaaatac aatttagatt taataactat
    19141 gatatgtaag atgaaactaa taatttatga ggtgtaataa tgatgataga taaattcaat
    19201 tatgaattat ataatgagtt tccacattta tcaccaataa tagaattgta taatttaaat
    19261 atagagtaca taagagaagt tgtaagtata cttatcaata cagatatgac atttcaaagg
    19321 tttaaatatt ccaaatctag agttgagtgt tctatgaaag cgtatattat ttcacctaat
    19381 agtaagctaa gtgttgtatt tgcatacgat tgtgaattaa atggaattga cttagacaac
    19441 ctgaaggcaa ttatatgagt ataaacaaat aaaaataata ctttacaaga aaggtaaatt
    19501 gaatatgtgt aagttttgtg aaagtataat tgataataca aaagatatac ttttaacctc
    19561 aagaagtaga ttattagaag acaatacatg tgaaattata gcagaagata attgtaataa
    19621 ttgttatgat ggatgttatg aatatttcaa actaaacgga tataaaatca ggggaaatac
    19681 atatgttaat gtcgactact acaaaaaaat tagaaaaata ataattgctc catgttcaga
    19741 atcagtacat ataaactatt gtccatattg tggtaaacaa gtttctaaag atataaaaga
    19801 ttttaattgt attcctgagc atatagtaga tgttagatat aaaaatggtg atgtatatga
    19861 ttatgatatg gaaaagtctg tacaagaagt catggataat gcaaataata attaaataaa
    19921 atgatacttt taatagggga taaatatgaa tctttggtct aaaattgaag aaccagtgat
    19981 gagagcagta agtggaatag tatttaatat atggggaaat gtttgttttt ccatatatta
    20041 catagaagaa aataaacaaa tagagttaga aatatatgat agaccttata gatatgaagg
    20101 taaaaaaaat agttgttgag aagttatttg attacaagtt aataaatgca gattataaag
    20161 aaatacatga tattgtttct atgatagaaa gatttttact agaaaattat taaaaggaga
    20221 tatttaaaat gaataaagga attaaaaact atataacagc agtgaattac ttacttgata
    20281 atttagagta tatgatggag gatgacgaat ttaataatga aaagttacag aaaagaattg
    20341 atgtaattag aaaatttgaa agagaattat aagatgggaa aaataaaaaa tggagaaata
    20401 gatactgaag attataggct ttttaaaaac attcaaaaag aaaaagatag agtatatagt
    20461 aagtacatta atgaagaata cataatggaa gaattcataa actacttaag attggaagat
    20521 aaagattata ctacagaaat attagaaaga tatgcacgat atattgatga aaatttagac
    20581 aatcgttgtc catattgtgt atataatatt tgtagtgaac atgataattg ttttgatgga
    20641 atacttgaaa atttaaaaga aaaaattaaa ataaaataaa tattttaata tgaaaggatg
    20701 ttgtgataat aagtataaga gaaattatgg aaatagatat agaaaatcta tcaatgaagc
    20761 agcatgaagt gattaaaata tagaattatt aaaaaaatat gatacatata tatataagaa
    20821 ggggcattat agagaaatga taaaagagag atttaaagca aaagatagct ttaatatgga
    20881 tgagttcact aaaatttggc ttatggattt taaagaagat tttgatttaa gcttaaagaa
    20941 aatgatagaa aatacgataa aaaaatcaat aaaagatact tatgaagaaa cagaaagggc
    21001 gataacaaat atagaaaatc ttataaataa actataagtc aaaatattac taacgacacc
    21061 tctaatataa gattagttat agaggaaatc tattattaga gaaggcatag attgcgtcac
    21121 tggaacaatt caagttaaaa acttagataa tcatatcaaa taaaatcttt tacaacaaat
    21181 caaaatttct aaaataaatt cttattaata attcaattaa aactaaagga gtgaaattat
    21241 attgaaaaag tttttattaa cagggttatt aggaacaatg ttaatagcca tgtctggatg
    21301 tacaggaata caaagttcaa tgaaagatgc tgaaagtgat gtatcagggc ttaatagaac
    21361 tgtaaatgta tattcagata atggcgaggt attaaaaaca tatacaagta aaagcatgag
    21421 agttaaagat ggagatggtg gtactataac actagatttt gatggtaaaa gggtgattat
    21481 atgtaatgct catgtagtaa tagaagaaaa ttcatctaat aaataaagga gagttaaatg
    21541 tttaaaattt taaaaacaat tcttataata atttgggtag tagatatttt aaacttccca
    21601 caatttcaat tcttagatac aacatatcca atcaatacct tagcatggtt gctaatctgg
    21661 attcttatac catcgagtag tatatatatt gacaaaaaag aataaaagaa aggagtatta
    21721 atttgatttt aaatattgct acaattttta atttaggttg tatggtggct aattacttgc
    21781 aagataatac taatgggata ctacttaatg gattttgtct tatagcatta ttattattga
    21841 ataatgactt agattaaaag tattatttta aggagattaa aagatgaaaa taacaatatc
    21901 aattgaagaa gcaatggata ttattaaaca acactatgag aataaaggaa ttgaaatcaa
    21961 agacttaagt tttaatttag aaaaaactgg aatggttaga aatattaaat ttaagggaga
    22021 aataatactt aagtaggaga tatttatatt tgttaaaaaa aaggggaaga tatttataag
    22081 tagtacaaat agaagtgatg ctagaagttt ccatataagt gattattata gaactccaat
    22141 tactaaaatc aaagagtttt taactgagtt cagtagatat gaaaatatac tcaattcaaa
    22201 tataaaaata ttagacccat gtgcaggtgg agataataca aatccaatga gctatccaac
    22261 tgcaatccaa gaattttcaa atcaagaaat ttcaatagat acagtagata taagagatga
    22321 ttcaagagca aatataaagc aagattattt aaagtttcag ccaaaagaaa aatacgatgt
    22381 aattattact aatcctccct ttaatttaag cttggatata attaataaag ctttaaatga
    22441 tgtaagagaa ggtggtttcg ttattatgct gctaagatta aattatcttg gaggaaaagt
    22501 aagacaaagg ctgtggggaa ataatatgcc taaatacata tttgttcaca atagaagaat
    22561 gagttttaca gatgatggaa agacagatag catagaatat gctcattttg tatggcagaa
    22621 ggggtataat ccaaaatttt ctcagttaaa agtattaata agtcaatgaa atagaggtgt
    22681 aaagtttatt agagatggaa ataaatagaa gaagatgtga gtatgctgcc agaacaggaa
    22741 gatatcctaa ttctcttatt ttgtgggaag gttactatag ccttcttaat atccaatata
    22801 tagaggataa aacaagagga tatattacta aatttaatgg catggatgtt ataaaatgta
    22861 atgacttttt aaaaatagaa ttatttgaaa agaatattaa taattaaaat ttgtttttta
    22921 agttgaggtg atgagttgag atataataat tatcatagtc ataaaattta ttcaaatata
    22981 aggtcgttag atgtaattac taaaccacaa caatatatag atagagcaat aaaactaggt
    23041 caaaatacat attttacgac agaacatgga tatcaaggaa atgtatatga ggcaaaaact
    23101 ttatgtgatg aatataaatt aaaaatgatt attggagcag aattttacta tgtaaatgat
    23161 ataaatgaga aagatagagg aaattatcat ttaatagtaa tcgctaaaaa taatgatggt
    23221 tacaagcaaa taaataaagc tttaagttta gctaataaaa atggatatta ctataaacca
    23281 agaatagatg aaaaaatatt atttgagata tttaaaccag gggatgtagt tattactact
    23341 gcatgtgtag ctggaatatt aaaacttgag aatagagaag aattaatact aaaactaaag
    23401 aattatttta aaaataattt ctttcttgaa gtacaatctc atcctcataa aactcaagct
    23461 ttgcacaata aggatgtttt agaattaagc aacaaatata atatagatat aatacacgcc
    23521 aatgacagtc attatattta tccagaagaa tcaaaatata gaactaaatt tcttaaagct
    23581 aaaggtataa attatcctga agaagacggt tttattttag attatccaaa ttcagaggat
    23641 atatttaaaa gatatgaaaa acaaaatata ttaacaaagc aagaagttga aagagcatta
    23701 aaaaatactc taatatttga tgagtgtgaa gaaatcacct taataaatga tgatataaaa
    23761 ttaccatcaa tatctaaaaa tccaaataaa gaactgaaag aaatattaaa taaagaatgg
    23821 ttagagaaaa gaaaaaatat acctaaaaat agatggaatg aatacttaga tgctataagg
    23881 tatgaatttg atattataga gaaaactcat atggaggatt actttattat agattataaa
    23941 atagttcaaa gagcaaaaca agaatataat ggtcttctta ctaaaacagg gagaggttca
    24001 gcaccttcat ttataataac taatctttta gggcttactg aaatagatag attaaatgct
    24061 ccagtaccat tattccctac tagatttatg agtgttgaaa gaatactatc agcaaaatct
    24121 ttgccagata tagatttaaa tgctgaagat gctgaaccat ttatacaatc aactagagat
    24181 ttattaggag aagaaaattg tgcatggatg ataagttata agccattaca agatgcttca
    24241 gcttttaggc tatggtgtaa agcaaatgat atgaaactat ctgagtatga tgaagtagct
    24301 aaaaacttag ataaatatac aaatgatgtt ttttggaaag atgtaataaa ggaatcaaag
    24361 gtatttgtag gagtagtaga aagtgtttct ttctcaccat gctctatgct tatatatgat
    24421 agaccaattg atgaagaagt aggattatta aaaactaagg atggggtatg ttgtaatata
    24481 gatggatatt attgtgataa gtataaatat ttaaaaaatg attatttaac agtgaaagta
    24541 tggtcattaa taagaaaaat ttgtgaatta gcaaatatta gtattcctac aattgaagaa
    24601 ctaaacatct tattagatgc taaaacctat gacatatata aggacaaatt aacttgcact
    24661 ataaatcagg tagattcaga ctatgctaca aacctggcat ctaaatacaa aatatctagt
    24721 gtggcggaaa caagtgcatt tgtagctagt atcagaccag gatttgcatc tttattagac
    24781 aattttatta acagagagtc atatacaact aacgttaaag aactagatat attattagaa
    24841 gatagttatc attatcttat gtatcaagaa tcaataatga aatatttaat atggttagga
    24901 atagaggaat ctgagagtta tgatattata aataagatta aaaaaaagag atttaaggaa
    24961 aaagagttga aagaattgca tatcaaacta aaaaataact ggattaaaaa agtcggaact
    25021 gaaaataatt ttgaagatac atggcaagtt gtaaacgatg ctgcctcata ttcctttaat
    25081 gcttcacata gtctttcata tgcatatgac agcttatact gtgcatacct taagtcacat
    25141 tatccattgg aatattatac tgtagcattc aatttatata atgaagatac agagagaaca
    25201 agaaaattaa cagatgaaat agagtatttt agtattaaat tagaaaatcc taaatttaga
    25261 ttttcaaaat ctgaatactt ctttaataga gacacaaata gtatttacaa gggcattgaa
    25321 tctattaaat ttttaaattc tgatatagga gagtatctat attctcttaa agataataaa
    25381 tatgattcat ttttagagct attaatagat ttgcaggggc atataaactc taaacaatta
    25441 tctatactga ttaagctaga tttctttgag gaatttggaa agtccaacaa acttttagag
    25501 acttatgata tctataactc aatctatagt aaaaaacaat ttaaaagaga taatttacca
    25561 tgcgatataa ataccatgag aaaatactct aataaagaaa cagaaaaaat atttaaagaa
    25621 gttgatacaa aagaattatg ttcttatctt gaaagtcaaa ttgaaaatac agatatacca
    25681 accaatgaaa aaatacaagc tcattttgag tttgtgggtt catgtaacat aaaagatagc
    25741 aattcaaatc caaggtattg tttagtaata gatatagata ctaaatattc tcctaaagtc
    25801 acattgtata atataagttc aggaaatata aagattttca aagtaagtaa acctattttt
    25861 gaggataata aaattgatgt ttatgattta gtttatttaa aatcaattaa agaaaaagct
    25921 aaaagtaaaa aagtagatgg aaaatggatt aggagcaata cagaaacaga atggtggata
    25981 caagaatatt ggaaaataga aaattaagaa tatgacctcg tatatgcgtt tctaagagtt
    26041 tacataagag tctgcttgta tttatacatt gatttcttaa aatggcatat acgaagttgg
    26101 tgaaaggtag tgatttcaat acttatagat gaacaaagat attatagctt attggaagaa
    26161 aaaaaagacc taagtatgag aatcgatgaa ttaaaagagg aaaatgcttt tttaaaacaa
    26221 gaaaatgaag agtatgaaga aattcttaat gagtgggaat gtgtaagaaa agaaattgta
    26281 gcaacttgta aaaaaaaaga aatattaggt gaaaaaccaa gtgaattaga aatggttctt
    26341 caaaaaatag aggatatata atgagtgtaa ataaaattta tgagaaaaaa gaatacatta
    26401 ttatcaaatc aaaatctgga tatatagttt ataatacaaa taaagagttt aaagaaggcc
    26461 acactcactt aaaaagttat aatgctgcta aaacagctat agatttagtt attaagagaa
    26521 ggcttcctaa aagttattct atttattttc ttacaagttt gatgagaata tctaatgatg
    26581 tacaatatat aaaacatata agcgatattg tatatataag aaataataaa ggactaaaag
    26641 aaaaatatat aaattctaaa aatattaaaa aaaactattg agaacataga aggtatatga
    26701 tatactatga atataacaag aacatagaag gtaaaaagga ggtagtgatt aatatttgaa
    26761 atcttataaa gaaaaacagt ttttagtatt tgaatttgaa gatgggaaga atgtaaagta
    26821 taatttggca acaggagaat acgttggtaa atctggaagg attgtaaaag atgtgtgtac
    26881 tcaacttaga ggatataatt tacatgaggt aataaattct tttgaagatg aaaattataa
    26941 gtgtttttta aattttgtag atggaagagt aaatagaagt aagagtagca gagcttgggg
    27001 aaggagggtt gaaagaataa gaaatatagg aagtttttta aatgaaataa ataattattc
    27061 tgagtatgaa caattattct cagcaggatt aaaaaaaata atttatccga tacattgtga
    27121 tattaagagt atacctaaag gtcttatttc tatctgtagg aattatagta ttactttgag
    27181 tgaccgattg atacaagggt atagtaaaaa cccaaatttg tataataatt tattaagttt
    27241 agaattgaat tcaataagaa aaactaattt aatttcaatt cttgattctg gttatgatag
    27301 aatgatattt gataggttaa tagacatata taaatataaa cctcaatcac ttataaatta
    27361 tatagataat ctgatgacat atgaagcatt aaatgggctt gaaagcactc taaacgaatt
    27421 atatgactat gttgttatga tgagtgtaat aagtgataaa tatgaaaaat atccaagaca
    27481 tttcttaaca actcataaaa tagcatctcg aaattataat cgtcttaaaa ctcaatttga
    27541 agaagaagta tttaataaaa gaatagacaa gtcactagaa tattcatatc aagattataa
    27601 atttatatac cctaaaaata caaatgaaat taaagatgaa gcagtacaac aaaataattg
    27661 tgtaagtagt tatattcaaa atgttataga tggaaaatgt catatattat ttatgagaaa
    27721 aaaggataat ccaagtaaaa gtttagtaac aattgaagtt agaaatggca aaatagtcca
    27781 agctaaaggt aaatataata gagatgtcaa tgaaagagag caagaagcaa ttgataaata
    27841 taataataga atagaaagga tgaagaaaat atgttaatag aaggaactaa aataagatta
    27901 atgaaagaaa tacaaggttt tgagatgtta aaaataggag atatatttgt tattacatct
    27961 gtgggaaaca atggagctat acatttcaaa acaaattatg ggatgggatt catgaattat
    28021 agtgaattaa aaaaatattt tgaagttgca atagagacta agaaaaaaga acatatttgg
    28081 agcgaatgga gaactgcgac tgtatttgga acaaataaaa cttgcacata tagaacaaat
    28141 ggaaagaaag tagaagttag aatgggagat tttaaagcat cagctacttg ccatgattca
    28201 gatgagtttg aattaaataa aggtgttgag ttatgtctgg caagaattga agttaaaaaa
    28261 gcaaataaac aggtgaattt agtattagat aaaataaata ataaagaaat ttaggaggtt
    28321 agaatgagtt gtaaatttaa agaaagatgt gagaataaaa atagagattg cgattattgt
    28381 tgttataatc caaatgcata tttagaagat gcttttgaat ggaatggaga agggagaaag
    28441 ccaacagacg aagagttaga tgatgctctt gaagaataaa taagtattta aaattatcat
    28501 tttaatatga ttggaggtct ttatgaggtg gagaaatata ttagtaacaa gtgttttagt
    28561 aatgggtagt agcatacata tatttgctaa tactaaagaa atgagtacta gcaggatgga
    28621 taaaaatatt gtagcagata cagctaaaaa tattaactta atgtataaat atgagtggga
    28681 gcaacacaaa aatagttctg aatataaatt agaaatcaag agaattaatt tagaaaagga
    28741 cttaggaata aaaatagaaa aattgattcc tgtaacatgg gaagtaagtt actatacttc
    28801 tttaaactgt gaaaatagta aatatggagc tattacagct acaggagaaa aattacaata
    28861 tgggtttgtg gccaataatt atcttaaatt cggaactaaa atattagtcg atggaaattt
    28921 aaaggtggtt aaagatagag gttctaataa atattttggt aattctaatg ctatagatgt
    28981 atttgtacca aaattagagg gggagagtga ttataaatat tataagagag taaacaacat
    29041 gggaagacat tataaagaag gatatattat agttgaaggt taggagttga taaattgaat
    29101 gtaaatggaa taaaaatacc aggaattatt gatgtgtcaa atgtgtatgt ttatggaatt
    29161 aaagaaagca taattgcaag tggttatccg atgtatgaac aacaggtaat agatatgaaa
    29221 aaaataaaca tttcagaaaa agatttaaaa cgagcaaatc aattaggcaa atcaaaacaa
    29281 ggttcaggtc atgattgctt ttctaaagga attatcattc aaatggattt acaatggtca
    29341 gaatatatgt ggagacaatg ggacagatat catcataatg attatgtgtc tagtcaaagt
    29401 aaaatgcata gattatctaa atttaatctt gatgatatgt gtaataaata tgttgataaa
    29461 aatacaataa attttctaaa tgaattaatt catgagtaca ataactttga agaattgaaa
    29521 ttgaataatt ctaataatat tattcttaga aatggagaaa aagttgaagt cacaaaagaa
    29581 actctttgga aaatcataat atcaaattgc ccatcaggat tgatgctaac tgcaagagtt
    29641 acattaaatt atctacaaat aaaatctatg ataaatcaaa gagaaaatca taaaatggag
    29701 gaatggagaa ttctgtgtga cttttttaaa acactaccaa aatataaaga attaatgatg
    29761 taatagtttt gttagcaatt aaattttaaa tataaaggag aagtgggtat ggcagaaaaa
    29821 agaataaatt ataatcaagt atttgtatca ggagaagttt tagacatttt aaatatagct
    29881 agaatgaatc aaggaactgg ccaagaagct ataagattta ccttaaaggt tgaaacagca
    29941 ccaaacgaat cagtaaatgt agattacttt acatcaatgt ttaagtcaga tggttcacca
    30001 aatcaaatgt ttttaggaat agagacaata gctaatgaaa taaaaactag agcagaagat
    30061 ggaaaaggtg atattgttag atgtgtttgt tcattagaca ataacttata ttataaagat
    30121 ggtgaaaaga aagagcgatt ccaaatatca ggaacttttt gcaatagaga aaaatatgat
    30181 gacaaaggta atcctattga tgacaaaggc gaagtagtaa agtcaataaa agcgagtcag
    30241 gtctggagag tatatacatt aattgaaaat atagaagaaa aagaagatga tttaggtaag
    30301 tatttagaga ttaagggttt aattaataaa tatggtaagc aaggttgtaa tatgacattt
    30361 agaatacata atgaagatat gatagaagga tttaaaagtt tatataaaat aggagatgtt
    30421 ggccttcttg aaggtacagt aaaatctatg gttgttagta aatcagcagg atttggttca
    30481 agaattaaaa aaagtgcttt tacattttta gaaatagaag gtggcgatga acctttgaaa
    30541 gatggagaca aaatatttag tgataaaaat tatcctttca cagataagaa tatagaggca
    30601 atgagagaaa aaataaaaga aaaagataaa aaagaaaaac aaagagatat tgataaaaat
    30661 ggtaagacaa ttgagataag agatgatgat gtaccatttt aaattataaa ttaaataatc
    30721 tattaaaaag gagagatgtg aatggaagtt aaagaaaaaa caatgaatga tggtttcaag
    30781 aaagtggtaa atgaggaaat aaagacgtat atatcaaatt tatattcaga tagaatagac
    30841 ggattaaaag agagtgatag tcaaattgag ataacaaaag cattaatagc ctttcattta
    30901 gatatagaat caattccaaa ggataataca aacccatttt tcaggtctaa atatgcaaac
    30961 cttgatacaa ttcttaaagc agtaaatcca ttattagcaa aacatgattt aatactaatg
    31021 caatcagcag aagatgcagg taatgataaa gtatatataa aaactaaact aaagcattca
    31081 tcaggcgaat acatagagtc aaattctgca ccatttaaac cagctaaaac taatgatata
    31141 caagctagag gagcattgga aacttatttg agaagatatg cagttcaatc agtattagca
    31201 ttatcattcg aggaagacga tgatggaaat tctttaacaa acaaatcaaa gggtaaatca
    31261 gaagaagaaa attcaactat taagaaaagt agagtgtaaa taaaattgct acctcttaaa
    31321 tgaggtagca ataaatacat aacataagga ggggaaatct tgaattgtaa gtgtaggttt
    31381 tgtaaaaaga aattaaatac aaatgatgct tataaagtag agcatattac atctggggga
    31441 aataagcaga atagatatta ttgcaatgaa caggaatata gaaaagagca acaggatata
    31501 tatttctgga aacaatgtca acttggaata gattatatta tgggatatac agttataaat
    31561 aatcaaaaaa ataagatgtt acaagagata ataaaaggtg gttatacgag agaagaatta
    31621 tatgattgca tcattgagaa gaaagatgaa gttatagaat tacttaacta tagaaaagat
    31681 atagaagaag aatatcctaa attatgttat gtgtttacaa tattgaaagg aagtattaga
    31741 gatataacaa ttaaaaacaa gcaaatgaaa tatgaaaaag aaaatgaaag gatatacaaa
    31801 gaagtagaga aaagtgaaga atattatgaa ataattgctc ctaaaaaagt aatttataat
    31861 aaaagacaaa gtttatttga caaagtgaaa ggagtgtatc aagacgagta gttatgaaaa
    31921 aataatagag gataggggta ttatagaaac attggtttta ggcacaatgt taaaatcatt
    31981 aactcttttt agtgaatata aaattagcga aagtgacttt ataatagata aggtaaaatt
    32041 ctttttctcg ttaggaagaa taatgtctaa aacacataac gagcttgatg aaataagtgt
    32101 agctaaattt gtatcttcaa ataaactcaa atctgaatat gaaagatatg gtggttggaa
    32161 tagtttatca tctgctatgg agtatggaaa agaaactaat atagcagcat atattgatga
    32221 tttagctaag aataatttac taattgcttt agataaaaga ggttttaatg taataaaaga
    32281 aatggagcat aatgggttaa aatttattcc atttgaaata tttcaatcta tgaaatgtag
    32341 agaagtggaa gaattttatg aggggttaat atcttcatgt agtgtaaatt ctataaaaaa
    32401 taatatgaaa gttgaaaatc ttttacttac taaagatgat agagaaaaat taaagcaaaa
    32461 aactgaagca ggaacacctt ataatattat atttgaatac actgaaaaag aaataggatt
    32521 gagtaataat gaagaatcta agtatatata tagcttacca atattatcaa ataggactaa
    32581 tgggattggt aagggtggtg gtataaatat aatagcaggg ttttcaggca taggaaaaac
    32641 tactttacta ttttttaatt acatattagc tatgatatat agaggagaga agatagtcat
    32701 atttgcgaat gaacaaaaat ctcaatattt taagagtatg ttagtatcgt ttatagcata
    32761 taacatattt aattatcatg atttagatag aaataaaata gataatggag attttacaga
    32821 ctttgaagaa gacttaatgg aaaaaataga aaaattttta agagatagat gttttgcaga
    32881 gaatctaaaa tttatctata tggaagaatt tgaaatatct gaaatactaa ggaaaagtaa
    32941 agaacttgtt acacatcagg gatttactgg aatagcagtg gatacattta aatcagaaga
    33001 ttcgtcagat gcacattata caggaaaatt aattgaaaat tctaaattac tcgatagttt
    33061 tgggaacaaa tataatgtaa taactatgtt atctatgcaa ttacttacag ctcaagaaaa
    33121 taaaagctca tatctttcgg caggagattt gtcagaagcg aaagctgtca aaaatgtatg
    33181 tggtttatta aaacttatga gaaaagtagt aaatgagtta gaattagatt ctactaacaa
    33241 gaaattcttt ttaaaaccat ataaattaaa atataataaa ttaaaaaaga ctactgaaag
    33301 agaatatatc cagtttgatg caaaagattt gcaaaaagaa tatagattat tatttttaaa
    33361 taagtcaaga agaggtcaag atggagatgt aattttacta agattttatg gtaagactgg
    33421 tagattagaa gagataggaa ggtgtgagaa ggtctacaga ggacaattgt catactaggt
    33481 gatgactatg gaaataaagg atttgactaa tgaacaggtg ataaatttta tggaatattt
    33541 aggttcggac ttatctccta aaagcaatga tagacagtta atattcaata cttgtttatg
    33601 tcataatggt gattcataca aattatttta ttatacagaa agcaaaacgt ttcattgcta
    33661 tagttcttgt ggccatatag gtagcttaat agatttatta atacacataa ataaatatga
    33721 gtttaaagat gctattaatg aaataaaaga tttctttggt atttcaaatc aaccaatgct
    33781 aagaaaagga tttagaataa agaaaaaagt agaacagata catgatatta aagatataca
    33841 aatagaatta cttcctaccc ctaaaaagcc atatgtatat aaaacttttc agcaagtccc
    33901 aattgaagag tgggaaaatg aaaacatatc ttttgaagtc cttaaaacat atcaagtata
    33961 ttataatcta tatgaaaatc aaatagtaat tccacatttc tgttggcatg atagagctag
    34021 gcctgttggt attagagtta gaaacttaga tgaagataag gctaaaagtt ttggcaaata
    34081 tattccatta tggtacgaca ataggtgcta taatcataga ttaagtctta atctatatgg
    34141 attaaatgta aataaaaaat ctatcaaaaa ttccaaaaaa gttattgtat ttgaaggtga
    34201 aaaatcggtg ttacaaatgg ctactatgta taaaaataat ccctctgtag ctatatgtgg
    34261 gagtaatttt agtagagagc aaaaaaagat attaatagat ttaggtattg aagaattaat
    34321 catagctttt gatagacaat ttaaagttaa agatgatgaa gaatatgtta tatggagaga
    34381 taagatatat aaattagttc aagatattaa agatgtagtg aatataagtg taatatggga
    34441 taaatataat ttattgggct acaaagatag tcctagcgat aaaggaaaga caatatttga
    34501 aaaactatta aaaaacagaa ttaatataga gaactttgtt aaacaataaa aaagtaaaga
    34561 aattaatttt atgagaaaat ttttattgtt tagggattta ttagcagatg ctaaataaaa
    34621 tatgtgtttt aatatgaatt ggaggtagat tacatgataa aatttttaat aaaaaacgta
    34681 cacatgggag ataaattata ttgtataaat aacataggaa attataaaaa aggtgaaatt
    34741 tatacagtga gtgatactag tttttttgga ctctttcata aaactaaagg agaaaattgc
    34801 ttaccaattg aatgttattt tatacactta ccaaaaaaag aagagttaga aagtaatcct
    34861 aaatcaatga ctaaagaaga aattgaagat gaactaggat ataaaattaa aatagtttct
    34921 aaataataaa gtgaggtgat attatattga gtatcttcaa taaaaaatat ggtagaaatg
    34981 taaaaataac agaagaagaa cctccttctc ctcctcccaa acaaccaata tataatagag
    35041 ataatataca atatgataaa gaaatggaaa gaaaactatt agatattgat aggttaaaaa
    35101 tagaattgga aaaagaaaaa aagatagaag aagaaagaaa aaaagtagaa agagtaaagg
    35161 aaaatgaggg aaaggaatgg gcttgggttg aaggatataa atgtacagat aaaaatatga
    35221 aatgtaagga atttcaatat gaattaaaca aagcctatag tatggatggt gatgatatta
    35281 atatatggaa ttggggattc catttatgtt tagacttggg tgacgtattt caatattatg
    35341 ctccaaattt aagcaataga ttttttaaag taaaagctct agtgaaaaaa gccgataaat
    35401 ataaatatgg taaagaatat ggtgaagtga ctctcctttc aggtagtgtt ttacgtggtc
    35461 ataaaataaa taaaatagct gctaaagaga taatattttt agaagaatta acttttgata
    35521 aattaaaact acatatacaa aatagttttc ctgaagtaaa cactaagtct aaatggtatg
    35581 ccatatgtaa attaggagaa caagaatatt atcatagaat gtttatgaat caaatgaaaa
    35641 agattagatt ttcagataca ttttctcaat tattatttga tgaatgtcgt aattattgtg
    35701 aaatattaga aatcttaaat aaagcaatag catattcaaa agaaaattta tctaaagata
    35761 tgataatata tttgcttatg aaagatataa gaaagtaaca tataaaaaga agggagataa
    35821 tgtggacaaa ataaataaga tagatttgat taagttaaaa gaaatgaata tatatgataa
    35881 ttatcaatta aaaaaaatag caagtaaaat gatattacat ctaagatatg aaataatgga
    35941 cttttgtggt tgtggttctc ctgaagatat atcttttatg ataaaaggag tattgactgc
    36001 catacaaaat aaagaaaaaa attgtaattt agaatacaca gaaagatgtt acatatttga
    36061 aatagaattt aataatgttt gtggaataat tggtagtaaa aatgatttga tacaagaatt
    36121 tatattaaat gctttaaatt catatgggtt attagaacat ggtagtagca tttgggggtc
    36181 atggctatct gattatggta aacaaatatt atgtgctttt gaaatagttg gtgactgtat
    36241 tttagatgtt agctatttag attaaaaagt tagttttaat aggaggtggt aattattaaa
    36301 tatgtaatga gtgacttaca tggtatgtat gataaattta tttcaatgtt agagcaaatc
    36361 aatttcaatt caaatgacca tttgtatatt ttaggagatg tattagatag aggggataag
    36421 tctttagaga ttatagatta tataagaaag tataagaata taactcttct aaaaggtaat
    36481 catgagttaa tgtttcaaga aagttataca gatgtaaata gtagttttct ttggttttat
    36541 aatggaggta aagatacatt ttatgattta ggaatgaagt cttatgaata taaagaaaat
    36601 ttttataaat atattaagaa ccttccatat ttagagataa tagataattt tatactagtc
    36661 catgctggac tataccttcc aaataactat gagaatctca gtatagagca aataattgag
    36721 ttacaagaag aagatatttg tgtgtgggat agaacaatat taaatactaa taaacatata
    36781 aaaggataca caattattat tggtcataca ccaactcaaa acattaaaga ttatgaaaag
    36841 gctgaaatat ataaacagga aggaattata aatatagatt gtggagcatg ttttgataat
    36901 ggtaaattgg cttgtttaag attggatgat atgaaagaat tttatattta agaggtgtta
    36961 tatggacata ttaaaaatca gttacaacaa tctaaatgct atatcaaaaa tatcagaagt
    37021 attaagggat attgtaaatg aggacacttt aatagtatgt ataggtagtg atagagtttt
    37081 agctgactca ttagccccta tgattggcag tatactagaa aaatctacta ttagaaataa
    37141 aatatttgga gtattaggag attcaataca tgctttaaat ttagaacaaa aaatacaagc
    37201 aataaaaaat aattatccaa attcaaatat aatagccatt gatgcttgta taagtaaaat
    37261 ttcagataaa ggaacaatta taattagcaa taaacctgtt aaaccaggtt tgggggtaga
    37321 taagaagtta ctagaagttg gtgattattc aatagttggg attataggta gaaataaata
    37381 tgatatatat gatacttcag accctgaact tatacttgat ttagctgatg ttatatcgaa
    37441 atctttaata tctatattat tagaaaaaga agaaaggatg attgtatgat acaggaagtc
    37501 agacaagaaa tactattaaa aaatggtttg attttatata caggtgattt ggtagaaata
    37561 aaatacaaat cagatgaaga tgtaatagaa catacttgta aaggtaaaat taaagaagca
    37621 aaagaattat ttattaaatt agatacaagt aagaaatata aaacttctga aagaatgatt
    37681 tattcttggg aattaaaaga aataaaaaag gtggatgatg aagatgaaaa agataattaa
    37741 tagtaaatat agagcattat atttatttat attttttcta attttcaatt taatatatag
    37801 tttatatttt ggaaggaata cggagattgg attttattct agtacatctt gcatagaaga
    37861 atatatactg gatattataa cctctatagg actatttttc tcaatgatgt tagctgggtt
    37921 tgatgttaca gaaaacttta taaacagttt aagggattat tgtgaagaag atgcaaagtg
    37981 aggtgaataa attggaatta aaggattata taagtaaagt agaattaaat gacatgccat
    38041 tgaccttaga caataaatac atgttaaatg aaataagcat atctgaagat agcaaaatct
    38101 ttgctgtaaa agacacatca ggaataacat cttattatac tgaaatagaa tttaataaac
    38161 tatttagagg aattaatagc atcctagaga atattatcta atatatattt ttaaggaact
    38221 gagggttgag agctattaat taatagctct ctattttttt gaaaggggag aattaatgga
    38281 aaaggaacta tacagcttta gtaaattaaa tgcatttaac acatgtccat ttcagtacta
    38341 tcttacatat gtaaaaaatt taaatcgaga acaaagttgt tacggttatt atggcaatga
    38401 attacatagt ttactagaac aattacaaca aagaaaaata acaaatcaag aagctattca
    38461 aaaatacaat gaagttatag aatatgcaaa tctaatggat tacaattttc ctacaccaaa
    38521 ttcaagaatt aattatttag aatgtatatt acattatata gaaaattttg taccaattga
    38581 atgtgataga tactatatag aagaatattt tgagtttgat attaatggaa ttacaatgag
    38641 gggatatata gacttatatt atataatagg taataaaatt tatgttatag attataaatc
    38701 gagtagtaaa ttttctaaaa aagacctacc taaaaaatca atgcagctta tcttatatgc
    38761 aatgtatctt aaagaaaaat atccagataa aataattgaa tatgtagctt ttgatatgtg
    38821 taaatatatg aataatgaaa aaggagtatt aattgaaaga aataagattg atagtataaa
    38881 tgattatgaa agagctatag taaaaattaa atatactaaa gaactagaga aacaattaat
    38941 tgattttgtt gttgatacag ttactcaaat aaaacagcta gattccaacg atgagagcgt
    39001 ttggtgcaaa agtgatgaca aaagtaatca gtttttttgt aaaactttat gctctcatta
    39061 taacaagggt tgcaaacata aggaaaacaa gagttttaga aaatcaaaat cataataaaa
    39121 tgtagatttt aataagattg gagagtgttt aaatgaatat accaaagcaa gtaaaagtag
    39181 gaggtttatt ctataaaata gaagagacag aagaacctat tttagtaaat aatcaattat
    39241 gttatggatt agctgattat ggaactgaaa caataaaatt atctaataat ctacaatcag
    39301 aaagagtaaa agaagctact ttcttacatg agttattcca ttgtatattt agagatagat
    39361 gtatagagag tgaagatgag gaatatttag tagatgtatt aagcaaggga cttcatcaag
    39421 tgattattga taatcctaat atatttgaac ttaaggtgag tatagatgga taaattaaaa
    39481 aatatatttg tatttttagg ttattctggc tcaggtaaag atagtatagt aacagaaatt
    39541 tcaaagcaat tcaatattcc aattctaata tcacatacaa caagacctcc ccgtaattac
    39601 ttagaaatca ttaataaaac ctattatttt gtagataata aattctttaa gaaagaaaaa
    39661 aataacttta tagaaatgag aaagtatata gttcatgatg gcagcacttg gctatatggt
    39721 atacataaat ctgaattaga gaataaaaaa tatgctttga ctatagtaga tgcaagtgga
    39781 tataaagcac tagaaaaata ttttacagga acaaaaacta agttagtacc attttttata
    39841 aacgcagatg aagatactat aagaaaaaga ttaatagata gaggtgataa tcttaaagaa
    39901 atcgaaagaa gattagttga tgataagatt aaatttaaag agtttttaaa tagtgaaaac
    39961 tacattgtaa tagcaaataa cttaagtata actaatgcag tagagcaagt taaaatacat
    40021 atgaaagagg ggattcaatg gtcttagcct tagatgtatc catgtcctca actggatggg
    40081 cagttataaa tagaaataag aatatattaa aatatggaaa aatagtaact aaaaaagata
    40141 aattcaagtc tgaagatgag cgaatgtgtt atatctgtaa tgcaattcaa gagttaatta
    40201 taactcataa tattcaaata gtcttaattg aagaccaatt cacttcaaga aactctaaaa
    40261 caattcttag tttaagaaaa ctcttaggag caataatgag gactgtaaaa ttaaataata
    40321 tagagattga atatatgtat ccagtttcta ttagaaaata tctcctgaat aatggtaaag
    40381 ctaaaaagga agaagtagct gcatatgtaa gagaaaacat tattgatatt ggagaatata
    40441 ttgataggac ttgtaaggct aaaacaagtg atatatatga tgctatagca cttggaatag
    40501 cttatttaaa caaacttgat actaatagat aattttaaaa agggagatta aattaatgag
    40561 tataaatgtt aaaataaaga aattaaatca aaatgcaata gtgccatctt atgcaaaaga
    40621 aggtgatagt ggaatagatt tatatacact aaaagatacg gtgattccag ctaaatcagc
    40681 agtagcaata tctacaggta tatcattaga aattccatat ggatatgaaa tgcaagtcag
    40741 acctagaagt ggtataagct taaaaggaat gttttttaca agatatgtga ctatatatga
    40801 taaagtgcaa cctaaaaaac aaattagaaa aatttttgta gcacatccaa ctgtaagatt
    40861 aggtacagtg gattcaggat atagaggtga aattaaaata attacatata atgaagagga
    40921 ttttgatatt ttaattccta aacatactaa acttgcacaa ggagtatttc aacaagttcc
    40981 aagagctata ttaaaagtgg tagatgaatt gactagctct gaaagaggaa gtaatggatt
    41041 tggaagtaca ggtatataga tgaataagta tataatccta gaaaaatata attattacta
    41101 tgatgacaaa tgtatgacat tatatcattt agaaaatatc tatacaggat taagattttc
    41161 tattgaaaaa attgaattag agtactataa tatatcctag actaactttg ggaggagtat
    41221 taaatgtaca gatatgatga ttatgtaaat ttaataaatg acttagaatt accaaaagaa
    41281 attaggaata aatgtgttag agttataatg acactagaga acatgcagga tattatggta
    41341 gtacataagt ttaagactta tgttgttaat gaaaaatata ttgaaaaagt agcataaaat
    41401 aattaagtca tctattaatt taggtgactt ttttgcattt atgtcgaata tttcatctac
    41461 ttcatattgt attagatgct ataattaaaa tatatgtcga aaggtagatg gtgaggatgt
    41521 ttaaggagat atttgaaaag gtatttatgt cagaagaagt tagtaaaact gtaaaagaag
    41581 ttaataatat tattaaggat actaagatac aagtaaaagg tggatttgga gaacttagat
    41641 tagattttat acttagcaga ttaggcaaag attacataat aattaaagac attattgtac
    41701 caggctcaaa taagactaca caaattgata gtattgtgat ttctatgtat ggaatatttg
    41761 ttatagaatg taaaaatttt agtggatata tttacggaaa tgataaagat aaagtatgga
    41821 ctcaaattgt aggaaaaact aaaaatacat tctataatcc ggtaagacaa aattatgctc
    41881 atattaaagc aatagaaaat ataataggaa ataaatataa tatatattca ataattgttt
    41941 tttcagacaa agctacacta aaaaatgtaa tgataagtgg aaataatatt atagttataa
    42001 atgaaagtga gatattaagt acaataagta aatgtaaaga tgtgactatt gataaagaag
    42061 aaataaaaat tatcagagat aaaatttttg attgtatgaa aaccactaat caaaatacta
    42121 gagagcatgt tagaaattta aaaaatatta ctgaagagga taagtgccct agatgtcaaa
    42181 ttggacattt gattaaaaga aaaggtcagt atggtgaatt ttggggatgt agtcagtatc
    42241 caaaatgtaa atacactaga aaataaaaaa caacctctta aattcgattt taaggctttg
    42301 taaaaattaa tgcttataat taatcctgtt cggaaaatcg attgcttaaa atggaaaata
    42361 tataaaaata atagcgtgat agtgtaaggc atagaattgt gtattctatg tcttatttct
    42421 ttacaactaa gtaatttcaa taattatata aataaaagaa ttaaaaaagt cagttgaaag
    42481 tcagctaaaa tgactatttt ttgcctcctc tgtatggtat aataagtata tgaattttga
    42541 taggagggat agtcatgaac gcttttataa gaaaaagaaa taaaaattat gtagtgtact
    42601 tagaatttag agatgatgaa tcaggaaaaa gaaaacaaaa aaatatggga gcatttgata
    42661 aaaagagaga tgctaataaa agattggctg aagttaagga cagtatatat aaagatagtt
    42721 ttcttgttcc aaatgaaatc actctagctg gatttatatt agactttctt gaaaaatata
    42781 aagataatat ttcggcatct acatataaaa gctatattgc tatttgtaaa aatcatatta
    42841 atccttctat tggaaaatat cgtcttcaag agttgagaaa tattcatata cagaattata
    42901 tagatgattt agctggtaac ttaaatcctc aaactattaa agtacatata aatgtattga
    42961 gacttgcaat aaagagagcg tatagaatta agttaataaa agaaaatatt atagatggta
    43021 tagaaagtcc aagaattaaa aaatttaaaa atgaaattta tgataaagag cagatgctaa
    43081 aattattaga gttagctaaa ggaactaata tggagcttcc tattagttta gctataggtc
    43141 taggattaag actttcagaa gttttgggat taacttggga taatattgat tttgatgaaa
    43201 atacaataac agtaaataag ataactagta gattagatgg ttctgttata cttaaagagc
    43261 caaagacaga aagctctgtt agaaaaatat ttgcaccaat agagcttatg aatttactaa
    43321 aaaattatag gctggagcag aataagaaat tattgagaag tattgttaga aatgaatata
    43381 acttactatt ttttgataga aaagggaatc caattgctga agatgtaatg agtaagaaat
    43441 ttagaaaatt cttagagaac aatgatttac ctcatattag atttcatgac ttaagacatt
    43501 cacatgttac tttacttata aattccaaag tacctataaa ggttatatct gaaagggtgg
    43561 gacattcaaa tattaatact actcttaatg tatattctca tgtacttaaa gaaatggaca
    43621 aagaagcttc tgatagaata tctgaaaact tatttaaggc taattaataa ttattatcaa
    43681 taaatacaaa attaataata aaagcaaagg tataaacata aaggggaatt tttacaaagc
    43741 cttagaatgg catatagagc gtcagatttt agaagatatt aaaattttat atttccaata
    43801 tttatgatat aataaacata gcaaggaata aatatttgaa aaagctgtga gtggtgtttc
    43861 catagaaatt tcctcacttt cttatgaaag gaggtgaaaa actatggaaa atctaatgat
    43921 gagtattata gctggtgtta tagctagtta tatctacgat aaattaaaaa gccactcaaa
    43981 cgcgactaag agtggctggg aacttaatat taagttccaa agaaaatcca aataaactaa
    44041 tttagtttgg aaacatcact caaagttaag taaaatatgt ttccttgctt ttattatacc
    44101 acaaattaag aatattaaaa caaaatttca tgtaaataac atttaatctg taatttttct
    44161 attttcaaaa aaaactaact aaaagttgaa atataacaaa tccaactgtt gaacctaaag
    44221 catttatagg tactgatgtt atattagtaa agttatttaa agctgatatc cctagcatta
    44281 ctatagaaac tatagccaaa taagagataa ctaaaaaaat tggtattgat atacttatat
    44341 taatctctaa ttcaggcaat ttcataatat cattcctttc aacattctta tatttaacct
    44401 tattatacca ttaaatataa gaaatcataa tgtacaaaat aacttatttt taattacata
    44461 ctatgaaaga gagtgctttt aatggggtct atgttttata tatactattc cttaaaaatc
    44521 tatttaaaga atactatcat agaaacatat cttgttagat tttgagttaa aagctacatt
    44581 atttgattca tttcctatct gaaattatat tgtatgtaaa tcccctgaaa atttatggtt
    44641 aaatacatat tcttgattat caatagtaac tatttctgta aaatcttttt tagaagcatt
    44701 ataagtattg ttttcagtta gataagtatt gtttgtcttt gtaaaattaa cactcctatc
    44761 tatgaatgat aattcaacct aacttaatca tattgttgtc acacaaccta atgcaattaa
    44821 tctactctgt atttttttat cttgttatac cctctacttc aattctattt taatagaatt
    44881 ttatcatgta tagtaacaat t
//
LOCUS       DAMJVK010000024        44450 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.024, whole genome shotgun sequence.
ACCESSION   DAMJVK010000024 DAMJVK010000000
VERSION     DAMJVK010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 44450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44450
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.024"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            75..356
                     /locus_tag="PC414_001329"
     CDS             75..356
                     /locus_tag="PC414_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463731.1"
                     /translation="MASKQIPRNNLEKFLRIRVLSHTGDKIHIKLPVNFAKRMIENNA
                     LDLFNGKDDVVDGKKVTEILVKAFDYDLTGEVVHLERKNGDIIKVSIVQ"
     gene            620..874
                     /locus_tag="PC414_001330"
     CDS             620..874
                     /locus_tag="PC414_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463732.1"
                     /translation="MENKDFKLTICIFAFVIGVVVLFCCIGLGEFSAEHIIQQNGGIM
                     DTDQYNIYLDQSISQYRNFGSILALLGGIGILFDKYIRKY"
     gene            919..1242
                     /locus_tag="PC414_001331"
     CDS             919..1242
                     /locus_tag="PC414_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463733.1"
                     /translation="MATEIFTVKITPLEAQKMIKENLDADLVFSDSYNLGDDKFILIT
                     TFEKYYARNNSDAGLIVVYENTTGKTVIKATSTGSAIGILKIDWGAGGNLLKRVKNIL
                     ADYII"
     gene            complement(1801..1995)
                     /locus_tag="PC414_001332"
     CDS             complement(1801..1995)
                     /locus_tag="PC414_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2463734.1"
                     /translation="MINYMVEIIYSEKISEVENMLKIENIQYEFTSRDGFIFFGFYDM
                     TDAYRFETLCSDEKIRFKRI"
     gene            complement(2369..3988)
                     /locus_tag="PC414_001333"
     CDS             complement(2369..3988)
                     /locus_tag="PC414_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HDF2463735.1"
                     /translation="MNIVISLIGGLGLFLYGMSLMGEGLQKSAGDKLKKIIELLTSNV
                     VMGVLVGTVVTGIIQSSSATTVMVVGFVNAGIMNLSQAIGVIMGANIGTTVTAQLVSF
                     NLEGIAPIALGIGILFYLFTSNQKTKHLSEILIGFGILFTGMEFMKDAVAPLAEYKAF
                     TDALLYFSKNPVLGILAGFAITGIVQSSSASMGMLIALASQGILPLSSALPILYGDNI
                     GTCVTSLLSSVGASRNARRAAVMHLSFNVIGTIIFMLVLNKPISAIVTHFDPTDTARQ
                     IANAHTLFNLTNVIILLPFSKYIVKLANRLIPIKETESEIVNNTKYLDERMFSTPSIA
                     LGNTVQEVVRMGHKATNSLEHSIAGFLNKSNEDINKAFESEKVVNKLQKDILNYLLKL
                     SKEPLRDDERFRTDLLFNTVNDIERVSDHAENIAELAMSVKEMNISFSDSAIREIYEI
                     YNKTITNFKDALIVLDVKDFELANKVLEVENEVNYLEKTFRNSHMIRLNNGSCTIDAG
                     VLYLDLLTNLERISDHSTNIVKQVLKLKQKI"
     gene            complement(4297..4515)
                     /locus_tag="PC414_001334"
     CDS             complement(4297..4515)
                     /locus_tag="PC414_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2463736.1"
                     /translation="MFFSLFKKKSGYTFTNFLSKVRVEKSKKYILKKDLSILDVAVLI
                     GFNSQNYYSIIFKKFNNFTPIEYKNIYN"
     gene            4808..4999
                     /locus_tag="PC414_001335"
     CDS             4808..4999
                     /locus_tag="PC414_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463737.1"
                     /translation="MERLMELIERKFLKDVELLELEDIENVIECKMTQPYNAHYNEYL
                     VKVKNGDEVEEYFVYLKHR"
     gene            complement(5088..5900)
                     /locus_tag="PC414_001336"
     CDS             complement(5088..5900)
                     /locus_tag="PC414_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HDF2463738.1"
                     /translation="MADCNSCPSKGNCNSQSNCSIENNPNNKFGKIIGVMSGKGGVGK
                     STVTALLANKLNKMGYKVGILDSDITGPSIPRLMGVKNVKAYSDGSYIYPVENSNNIK
                     VMSINLMIDDENEPVVWRGPLLGGVVKQFYTDVLWEELDYLLIDMPPGTGDVALTVMQ
                     SIPISGIVMVSVPQDLVSMIVSKAVNMAKKMNINVLGVIENMSYIQCPDCSKKIKLFE
                     GESTEKFLDDLDLELLGELPMTKEIIDITHNGVTEISDDLDSILTNVVEKIK"
     gene            6322..7473
                     /locus_tag="PC414_001337"
     CDS             6322..7473
                     /locus_tag="PC414_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004452985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2463739.1"
                     /translation="MELRPRSAKLEEFDKVIELINYVFRISRNHKPTMMEEFPLLLSK
                     NNIENMIIISEDDKVISDVNYLIQDVSIQGNRLKVAAIGGVCTHPDYEKRGYSSKILD
                     KVEEKMFYDGVDIVIISGTRSLYSRRNCSLVKSFYKYTIKPEDVKIAYEIVEFDETNF
                     EKDNDLDKMIELYNQNSTRFIRTRDEFQKLLHAATIAWGPIGYKKVFIKENNNIIGYL
                     IIRTIKKEDSTVGEVAEIGLNSVNVENILKYVANKFGLEYLNYKVHVKNLKDQLKCNG
                     TKSLDYQQGTMKIINFTKLCDSLRSYFSQYVDFELLKYMEFKQVENKYIIKYKEEELV
                     IENLDKLNKLFFEKNEEQYNEFKHLKNIYEFATKAFPVDFPWTANLNYQ"
     gene            7591..8229
                     /locus_tag="PC414_001338"
     CDS             7591..8229
                     /locus_tag="PC414_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423639.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HDF2463740.1"
                     /translation="MDILDDIAECILNMGVDNIENLVKIAIDKNIDVEDIYEYGLNKG
                     MIGALDKFENKEYYLSEVIVCTDALNKGINLLKGTGKVKKKSKGVILMSVVEGDTHEI
                     GKNIVKVMVEATGYKVIDLGVNRKSEDIIEEAIKNNVDIIGLSSMMTTTMENMKSVID
                     ELNMIEIDKRPKVIIGGGPVSMEFAEEIGADGYSSNAPKAVKLINKLIGGEV"
     gene            8230..9252
                     /locus_tag="PC414_001339"
     CDS             8230..9252
                     /locus_tag="PC414_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HDF2463741.1"
                     /translation="MFSDMTLLQYINSEDRGFFLPDMGTNGLFLINKKAYEVYDSVDL
                     QLELAKTMDKYFESDFIYSFCDGITFCETLGLETLRPDYDFPSVLTHTITDRDKLRKL
                     DVPDPYTSGRMPLNIESLSVISKSIKKPLYESIQGPFTLAGQLAGATQLLRCIITDKK
                     FVEELLEFTTELVRRYAVAANKAGAKYISIAEPTSVTLSKDRFDEFIVKNLNKIYNEL
                     DCWKGMHICGDTRELLDNMLSCNIDAVSLDQILDYEEIAPMIPRDIVLIGNLDPIKLL
                     GRSKPDKIRRETLKLLKKMRRYDNFLCDFGCNCLNTTPVENLQAAIKAGRISYKELDR
                     IDIDEL"
     gene            9242..9862
                     /locus_tag="PC414_001340"
     CDS             9242..9862
                     /locus_tag="PC414_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463742.1"
                     /translation="MNYNSNKNKYLLKDINLDDIFIDKNIVLKFLGYGNRKAPDTISK
                     IVEREIRNINDILDIKIYISEVDINKTPKECKKAFAVLYTIGDKIDIKMNDYMENCNM
                     MAGLALDKIGIVCLDYINEKIKMYLKGKYDSLKISHEIYPGDKDFEVERQKDIYNYVK
                     NKYNSIEIEINDYHQLSPIKSVAMLILMGDEENLESRCSKCPRKCF"
     gene            complement(9986..10672)
                     /locus_tag="PC414_001341"
     CDS             complement(9986..10672)
                     /locus_tag="PC414_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HDF2463743.1"
                     /translation="MKTYKFIFFDADDTLFDFKKSESHAFKKLLSEFDLEFNFENYIE
                     SYRNISDKLWLDLEKNIITLNELKLLRFELFANKISLDVDSETLSKMYLNFLGECTFL
                     IPGAIDILQYLKKKYTIVIITNGISAVQNKRLENSKIKGYIDGMVVSEELKISKPNPE
                     IFKYALKKFNCHDKSSALIIGDSLTSDVLGGINSGIDTCWLNSNNSINYTNHIPTYEI
                     NTLIELKKLL"
     gene            complement(10686..11231)
                     /locus_tag="PC414_001342"
     CDS             complement(10686..11231)
                     /locus_tag="PC414_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2463744.1"
                     /translation="MNDLNLGEKIAEVRKKQNLSIRDLAKLADVTPSLLSQIERGLAN
                     PSVNSLKSIASSLNVPLFTFFVSEVDKKNLIVRHDNRKKVILPGSKEVIYEILIPDSS
                     GNLEFAIMDLAPNTSSCVDRITHNGDEIAYVLEGEVKLFMDDDEFTLSKGDSVKVPLG
                     TKHKWQNDSNSESKVIFAVIL"
     gene            11459..12706
                     /locus_tag="PC414_001343"
     CDS             11459..12706
                     /locus_tag="PC414_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDF2463745.1"
                     /translation="MQITTFGAIFGLLIAIILIIKKFQAVYSLMLGAFIGGLVGGANI
                     TQTVDFMANGAMNISPSILRALASGVLAGSLIKTGAVDKISEQIVKIFGEKRALFSIA
                     ISTMVLAGVGVNLDVSIITVAPIGLYIGRKLNYSKLSILLAMLGGGKAGNIISPNPNT
                     IAVADNFSVNLSSVMMANIIPAIIGVVITVILASILINKGNKVQSYEILEQREDLPSL
                     FKSLCGPIIAIFLLFLGNVSPIVIDPMIALPIGGIVTLIVTGNLNNSREYLTFGLSKM
                     QGVCILLLGTGTIAGIIQMSELQQSTIGALQFLNMPQFLLAPVSGILMSLATASSTAG
                     ATIASSTFHDAIINGGLSPISGASIVNAGSSVFEQLPHGSLFHTSAGSINMDIGERFK
                     LIPYEALIGIVMTIISTSIQLVL"
     gene            12793..14787
                     /locus_tag="PC414_001344"
     CDS             12793..14787
                     /locus_tag="PC414_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDF2463746.1"
                     /translation="MNKKKIVIIGIIYSFLVVFSLTNMYVNMEYNLNVFEYIKKSLPF
                     TEEEKKWLEKHKNLIYSSDQSSPPLRYKGKEDGQYKGIIVDLINSLSIQIGRDFYFKP
                     NNWWKESFVNSIDDSIKFFDLIPSKERANKFIFTDPIYTLSANILKDKKSQDINSYMD
                     LKGKTVAIPEGDYSINFLKQKIQDINILLTPDIKTGVNHLMSGKVDAVVGDEPVLRYY
                     INNYGLSNKYSVLSNPIYTKKAVLAVPKQYEELVSILNKGIFKLQKNGVYKDLKKKWY
                     STYNEVDDILYERGIVPSIYLFIGIILISIYVFYSYTYLLKIEIKRKTEQVIENKKTL
                     EATFNSITDIIMLVDENNNIVESNKVLYDFMGEMSYKIADLISMIKGVIENTFSENTN
                     KTSEIEIHNKILKINTFPVEYKKNNTEYIVVLIKDITNDKIVEAKLLRENKMISIGQL
                     ASGVAHEIRNPLGIIRNNCYLLKDNVTMEEVNDCVKSIESNVDRASNIITNLLNFARI
                     SDDNLEHINIKNFIENIVKLQYKMLQLKNVEIKIDCEHNLICYINGESLKHVFINLIS
                     NSIDAIHQDGKIIIYCYEKNHCLFIDFKDNGEGIKEDVLKDIFNPFYTTKPIGEGTGL
                     GLYITYNEIKKNNGDISVESKLGVGTCFHIKIPLNKEVTI"
     gene            14787..16109
                     /locus_tag="PC414_001345"
     CDS             14787..16109
                     /locus_tag="PC414_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HDF2463747.1"
                     /translation="MKILLVDDELEYGVVMKKILQKKGYLVDIALSGEEAINIIKKDK
                     NYDLVLSDVMMKNMDGVQLLDRIKSINRDIEVILVTGYGSIENAVDAMKRGALSYFIK
                     SNPIENLLEEVEKVKASKISVSVQKNNFEFTLESKNRDFNNIIKIAKKAACKDVNILI
                     LGESGVGKDILARYIHSISPRKNEIFVPVNCCSFSENLLESELFGHEKGSFTGAVDSR
                     KGRFELSNKGTLFLDEIGDIPLNVQVKLLRTLEDKSIERIGSNKSIKVDFRLICAMNK
                     EPKVEISNGNIREDFFYRISTITITIPPLRKRREDLTTLIEFFLNKYQIEHDKKIHSI
                     DKEVKDFLLNYNYPGNIRELKNIINRLVVLSEEGNLSKDNLNLISNNVYIDDKISIRP
                     LREIRKEFECEYIEKVLSLCGNNISNTAKKLEISRRQLTNKISEYNIK"
     gene            16388..17674
                     /locus_tag="PC414_001346"
     CDS             16388..17674
                     /locus_tag="PC414_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006807454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HDF2463748.1"
                     /translation="MKLELGNIFIKNVEFGEKTEVKDGVLYISSEEIEKIVLEDERIV
                     SVNVELARPGESIRIAPVKDVIEPRVKIGDESKIFPGIINKVKTVGSGRTHVLLGACV
                     VTCGNIVGFQEGVIDMSGPTAKYTPFSKTNNICIVIKAKDGIDTHDYEEAARIAGLKI
                     GAYVGEAGREVEPDEVIVYETKPLLKQVKEYPDLPTVAYVHMLQSQGLLHDTYYYGVD
                     AKQIVPTFMYPTEIMDGAIISGNCVAPCDKVTTFHHLNNPVIHDLYKRHGKDLNFIGV
                     ILTNENVFLVDKERSSDMVAKLIEFLGVDGVLVTEEGYGNPDTDLMMNCRKCSEVGAN
                     VVLITDEFPGKDGKSQSIADATKEADAVVSCGQGNLVVHFPAMEKIIGTLDYVEMMIG
                     GYKGCLNEDGSMDAELQIIIASTIANGYNHLTARYY"
     gene            17760..19067
                     /gene="grdB"
                     /locus_tag="PC414_001347"
     CDS             17760..19067
                     /gene="grdB"
                     /locus_tag="PC414_001347"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:18807..18809,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HDF2463749.1"
                     /translation="MSKIRVVHYINNFFAGIGGEEKADIPPEKRAGVVGPGIAFQSQF
                     NDDAEIISTVICGDTYFGENIESATQELLKMIKEESPDLFIAGPAFNAGRYGVACGTI
                     CKAVEEELNIPVITGMYKENPGVDMFKLDLHIVSTGNSAASLRKVVPIMTKLGLKLVR
                     GEEIGPPEEEGYIMRGIRKNFFHELRGSERAIDMLVKKMKGESFETEYPMPEFDRVTP
                     MKPVKDLSKIKLALVTSGGIVPIDNPDKIESSSATKYGIYDLTDMDSMSNKEFTTIHG
                     GYDRAYVLENPNLVVPLDVVRELEKEGVIGELVDYFITTTGTGTSVGNSKRFGEEFSK
                     KLLEDDVDAVILTSTUGTCTRCGATMVKEIERAGIAVVHICTVVPISLTIGANRIVPA
                     VGIPYPLGNPMLGEEESKKIRKNIVLKALNALTEDIEEQTVFE"
     gene            19344..20075
                     /locus_tag="PC414_001348"
     CDS             19344..20075
                     /locus_tag="PC414_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2463750.1"
                     /translation="MIRKKVYILISIAICDDESYMLNHLKENIELYMKQKNLLYNIEL
                     FNKVEDLLCNHEKFNIVFLDIQMEEINGMQAARKLRSYGNECFIIFVTVLNEFVFDAF
                     EVDAINYLIKPLSNEKLYKTLDKIVTKLDDNESNYITIQKGYSYWKLKLADILYCEVI
                     KRKIYVHLKKETVDYYETIENLERSLPTNFFRCHRSYIINLQYVYGYENGNAVMESGD
                     RIPVSRLRQQIFSKVMLEFMKNRRI"
     gene            20077..21435
                     /locus_tag="PC414_001349"
     CDS             20077..21435
                     /locus_tag="PC414_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF2463751.1"
                     /translation="MINLFSSDLFLKYSFFFSFIVYRILMVIVQYYFLINFTNFNSKF
                     WCFILYSIISIFLIIINTYFNLYNLFSSQILFFLMTFLFVKYIIKQSTVLSIVSTLLV
                     YLIEQLVYGFTAPLNYLIFTSDINTDIYKFDINMGLSSLITIIIAGFIYWYSSKKFNI
                     KVMNFDKYIAILMIPLLLIILFMQSFEYSSNINIDTSLGIVKLLLNTSITEEIQAFLF
                     SIIGTICVFFSLFTFKKLIQALEDDKERAIMNQQIHAQKNYIEEAKSRLSQTISFRHD
                     FNNHLAIVNGLLKKDQILKAQDYLNKLEKVTSDLSFSCNTQNIVIDALFNNKLSIAKQ
                     LGIEVDCEVIIPQSTNMNDFDLCIVFANAIDNAIKACRVVDKQNRYLKLSTKLEGGFF
                     MIEIKNSYNPINNYTSGSGIGLLNIKEIAKKYQGAISIDKTSDYFQINVLLVISLHCA
                     SI"
     gene            21474..21959
                     /locus_tag="PC414_001350"
     CDS             21474..21959
                     /locus_tag="PC414_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463752.1"
                     /translation="MYLKRESLIYRNEEVTKMKVGNINGQAPITNGLDSCQSEVQGLE
                     QKKKALQDEIEKVRSDKSIDGETAKLKIEALKKEMEEIENKIQELKGNKGKKTNKEEN
                     NVSEVNNNLLERKKEPSNNENVKQLYDVFERSNVDEQWDNTYRVLQDDKNLKVKFNRP
                     I"
     gene            complement(22614..22838)
                     /locus_tag="PC414_001351"
     CDS             complement(22614..22838)
                     /locus_tag="PC414_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDF2463753.1"
                     /translation="MTVYNLKLGQKGIIDNIAGNEKLMKRLLALGLIDGTEVEVKKIA
                     PLNDPIVIRFRGFDLAIRKSDAKNINLKNN"
     gene            complement(22935..24188)
                     /locus_tag="PC414_001352"
     CDS             complement(22935..24188)
                     /locus_tag="PC414_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:glutamate symporter"
                     /protein_id="HDF2463754.1"
                     /translation="MITLSFDIVQTLTLSIFFFLIGNLLKNKVNFLNNFCIPAPVIGG
                     LLFCFLNLFFKYFNIADISISGNLMPNFITFFFTTIGLEISINLIKKGGSVLFRYWIL
                     CGVLAFCQNILAITISKIIKLEPLLGLMCGNVSMEGGHGYSAAFGLTIENLGIEGAVG
                     VGLSAATIGLIMGGILGCPVAKFLINKYKLKPSSNIDLSIPRYNRNLKRFGSKFFRNK
                     NRIKNNNFSETITPSVFLEQVLLIFICINTGEIISRCFYITFNILLPSVVTCMFSAVI
                     FRNLNDKINILELNFKLIDFLKELSLGIFLTLSLMNIDLFELSTLLPPILLIVTFQVI
                     FIILFSIFICFRVLGKDFDSAIIISGLIGHGIGATPNALANMSSLTQKYGDSPKAFLV
                     VPLVSGFLLDAISIPCILFFINILT"
     gene            24448..25437
                     /locus_tag="PC414_001353"
     CDS             24448..25437
                     /locus_tag="PC414_001353"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HDF2463755.1"
                     /translation="MFENLPKIDLHCHLDGSVRVETMLDIAIKEKIDLPSNNMDEIKK
                     LAKVSFNCTSLDEYLEKFDLPLKVMQSKENLKRITFELLEDASRENVKYIEIRFAPLL
                     HTQKGMSVKNIIEGIIEGIREAESIYDIKGNLILGCMRTMTSKEALLVIEEGKSFVNK
                     GVVAVDLCGPEKEGFCKEYKDVFKLAREYGYKVTIHAGEAASGENVLDAINILKADRI
                     GHGVKIKDHKKAYNLVKDKKILLELCPTSNVQTKTVDSYEAHPFYTFYKDNLHVSINT
                     DNRTVSDINLSSELNVIFDTFKLGLEDYKIIYRNAVEASFADKETKEYLNSLI"
     gene            25509..26249
                     /locus_tag="PC414_001354"
     CDS             25509..26249
                     /locus_tag="PC414_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HDF2463756.1"
                     /translation="MKDYEDVVYIKLVKKELEEWKKNILKKPSIFDKASKGIQNKFNG
                     VLPDKYHEIITSSIKVMTKTVLFGYKYATKPSLRNMSLEEREKLVLEKAKTYKTTAMI
                     EGAGTGAGGILLGLSDFPLLLGIKVKFLYDVASIYGYDVKDYRERIYILNILQLAFSS
                     QQHINKTFKLMNNWDEYVNSLPEDINDFDWRTFQQEYRDYLDLAKFLQLMPGIGAFVG
                     FYVNGKLIDKLCETAVSAYRLRIRELKL"
     gene            26449..27600
                     /locus_tag="PC414_001355"
     CDS             26449..27600
                     /locus_tag="PC414_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HDF2463757.1"
                     /translation="MTNLPKQFDSFNEARQKGFINAKELKESGKKMVGVFCTFTPVEI
                     PMAAGATVVGVCGVSEEPIPDAERVLPRNLCPLIKSSYGHAITDTCPYFYFSDLLIGE
                     TTCDGKKKMYEELAKVKPTYVMHLPNTSKGEFAYKLWKDEMIRLKEEVEKSLGVTITE
                     EDIRTAIKDKNEERELLKEFYALGKLQPSALTGLELHNVLYQAGFKFDRAELKHSLRK
                     VIDDMKERYEKGECPVQKDKPRILITGSPIGGISEKIVKTLEDAGASVVAYELCGAIR
                     SNDLLVDEEIEDVYDALTQKYINIGCSCMMNNDNRIELLDRIIDEYNVDAVIDVVLQA
                     CHTFNIESYRIREFVTKEKNKPFMSLETDYSKSDTEQLRTRFEAFVEML"
     gene            27625..28380
                     /locus_tag="PC414_001356"
     CDS             27625..28380
                     /locus_tag="PC414_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyglutaryl-CoA dehydratase"
                     /protein_id="HDF2463758.1"
                     /translation="MFSIGVDSGSVATKGVLFDGEKIIKKIIIPTGWSPKSTSKQVYE
                     LLSSEIDKKDIKKVVGTGYGRGVMDFADKKVTEITCHTRGIYFLNKNIRTILDVGGQD
                     SKVINLDRDGNVFNFIMNDKCAAGTGRFLEITSNLLGSDIESIDTLAKGYEPVNISSM
                     CTVFAESEIVSLLAQNISTGEVAAGILKSIANKSTSMLARGEVIDEVAFTGGLAKSKE
                     LVKMIEEILGKKIFIAEDTQIIGALGAAVIGFR"
     gene            28572..30959
                     /locus_tag="PC414_001357"
     CDS             28572..30959
                     /locus_tag="PC414_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp13"
                     /protein_id="HDF2463759.1"
                     /translation="MKKFTSKKVTRFVALFLISILVMSTIPVSADNSNTLNQIKKAEY
                     SETYKQYLNDAKNGRTEKYNGIIPNPYQLEGTQIQSKGRTAPTSYDPRKLGLMTSIKN
                     QEDLGICWDFAAMATLESFLKLNNYGDYDLSEEHLRWWASDGEYGWSVNDMNGALNYE
                     AMGYLTSWSGPKLEKDIPYNGRVSKAQGAKKPTNMNTAPTVFNVTDAVCVSNDINSTK
                     NAILQYGAVTSGYYEDIKYQSDDQNSYYCPTKSFNTNHAISIVGWDDNYSKDNFNANI
                     RPSKNGAWLIKNSWGDYNSEGGYFWISYEDKTLMSDIDNFSIKGGKKPNDDEKMYQHD
                     YASIVPLISKKITAANVFDFNRGDETLKSVMFLTESIGAKYEVYYAPVVNGIPQENNM
                     KKLKEGTAQYSGYITVPIDSFDIPEGKGAIVVSIEGKNGESTIGSESNVPGYDTFKAK
                     ANLGESYIIDNTGKFFDINRDSNFYPCNFTIKAVTEKSSGESIPNESLIGSDRYETAI
                     KVSQNGFNSSENVVLVNGSSIVDALAATPFTSAINSPILLTQKEALNSKTKAEIQRLG
                     AKKVYLIGGENSISKEIEQQLKSLNISIERISGSDRYKTSLLLAQKLNGIKNVSQIAV
                     VNGVKGLADAISVGAAAAENNIPIILANEKSELQGADEFLNSLNIEKSYIIGGTASLS
                     NNLESKLKNPTRLSGSSRDETNSKIIDNFYKKDTLKNAFVVKNGIKNQNDLIDGLAVG
                     PLGAKTGSPVILVGDKLADSQKEVLKNKTLEKVTQVGGGANKNAFKELVKLKSAK"
     gene            complement(31190..31423)
                     /locus_tag="PC414_001358"
     CDS             complement(31190..31423)
                     /locus_tag="PC414_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDF2463760.1"
                     /translation="MATTTKNRFYDYVQENLNKYVEVHSYFSSCPLYGEIIEADSLSI
                     TLCSRYIDESNPTLNETYTLYLPLSSIISIRTL"
     gene            31712..32302
                     /locus_tag="PC414_001359"
     CDS             31712..32302
                     /locus_tag="PC414_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2463761.1"
                     /translation="MPKSYSDKEREYIIKRLKEEARLCMEQYGIRKTTVDELVKRVKI
                     PKGTFYLFFQSKELLFFEVLRDIHDSIQKEILYEINRVDESITCEQLTDIFMKFYRMV
                     DSTSILNLMINGEFEILVRKLPDSIIEEHFRHDDFEIGEIISCIPNAKNKDIESFSGA
                     FRAVFLTMLYKREVGSNCFEDALRLMINGLVIQLME"
     gene            32416..33261
                     /locus_tag="PC414_001360"
     CDS             32416..33261
                     /locus_tag="PC414_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2463762.1"
                     /translation="MIKVDDLSFSYTERDFLQNINFGVGKGEILGFLGPSGAGKSTLQ
                     KILIGMITNYGGSVIVNGVESKRHSNKFYENIGVDFEFPSLYEKLTAIENLKYFGSLY
                     SKKLLSIDELLKSVGLENEANKRVSEYSKGMKSRLNFIKALLHNPDILFLDEPTSGLD
                     PSNSKVMKDIILSEKSKGKTIILTTHNMLDATELCDRVAFIVNGKISALDTPHNLIMS
                     KGAIKVRYTYFDNGEKTSECFLNNTANDKNLNMLIEKNKLLSIHSSEPTLNDIFIEIT
                     GRNLQ"
     gene            33258..33941
                     /locus_tag="PC414_001361"
     CDS             33258..33941
                     /locus_tag="PC414_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2463763.1"
                     /translation="MRLKNLILGDIKFQFKYGFYFLYLFLSIIYICIINVFPTFMREK
                     IAIIMIYSDPAAMGLFFMGAIVLLEKSQRVLNSLAVSPVKVSEYILSKVISLGVISSI
                     VAMFIAITLNLDNIIISTIGTFFSSIIFSLLGLILASKASSLNQFIVLSIPIEIICFI
                     PPILNVLLDTKSYANLYPFNICISLISGDKNFIMINILILISIIIIIYFITYYFICSS
                     WKKVGGVKL"
     gene            33938..34654
                     /locus_tag="PC414_001362"
     CDS             33938..34654
                     /locus_tag="PC414_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2463764.1"
                     /translation="MIKIINAFKQEFEQIKRDAMLFIVCVSPILCGVFIKFGIPLIQN
                     ISLNKFYYQLNLEPYFLMFDLLLAFITPFMFFFASTMVILGEIDDSISRYLIVTPLGK
                     TGYLISRFGIPGILAFIITMILLIFFSLTKISFLLNLSISLLVLLQGIIISILVISLS
                     SNKLEGMVITKFSGVFMMGILAPFFILNKAQYILFFLPTFWISKAFKEDNYVYMFISI
                     VISLIWILLLFKKFSKKIVK"
     gene            34751..35014
                     /locus_tag="PC414_001363"
     CDS             34751..35014
                     /locus_tag="PC414_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2463765.1"
                     /translation="MKNKIKILREKLGLTQEQLGRLVGTSRQAINAIETGKNEPSIWL
                     AYDISRIFNEPIESVFLFEESERKSRAQISRGEYYGYKKDKNI"
     gene            34986..35441
                     /gene="def"
                     /locus_tag="PC414_001364"
     CDS             34986..35441
                     /gene="def"
                     /locus_tag="PC414_001364"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDF2463766.1"
                     /translation="MAIRKIRTFDDEILRKKSKYVENVDNKIREILNDMAETMYNTPN
                     GGGLAACQVGVLKRLVVIDLGEGLIKLVNPEIIKQEGEQIVVEGCLSFPEVWGKLKRP
                     KKVTVQALNEYGEKIEIKGSGFMAKCLCHEIDHLNGIVFTDKIIEHVKL"
     gene            35493..35954
                     /locus_tag="PC414_001365"
     CDS             35493..35954
                     /locus_tag="PC414_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HDF2463767.1"
                     /translation="MRLWHKDLIDVLPKNQLVSQWRELLAIKGSIDKKGTPNHLLVNK
                     VLNYSIDEFKFYTKIVHDEMLKRNYKPNELKYTSILKWKNRNFANDISNEHSLNLENL
                     YDDWHNKMYLKQCLYNLEEKATCGGIPINEWNILLCKYSKDYELWSGNIMF"
     gene            36004..36906
                     /locus_tag="PC414_001366"
     CDS             36004..36906
                     /locus_tag="PC414_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2463768.1"
                     /translation="MYNKIKYIPINCEIACNKLKRSMPYKWDLNIYRGCEHGCKYCYA
                     IYSHKYINSNNYFEEIYVKTNIVEKLERQLKSSKWKKEVINIGGVTDSYQPIEADYKI
                     MPEILNLLIKYKTPAIISTKSDLILRDYDLIDKLSRITYINVASTITTVNEDTQRLIE
                     PNGVDSMRRFKMLKEFRKTNASVGLHIMPIIPYITDDFDNINSLFRHAKECNVHYVLP
                     GTLYLRGITRGVFFDFVKKEFPELFDKLSILYSEGSLNREYKNQLYKMVNELRYKYSL
                     SSSYSKIMKEKLKNSGDVQISFFD"
     gene            37276..38088
                     /locus_tag="PC414_001367"
     CDS             37276..38088
                     /locus_tag="PC414_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF2463769.1"
                     /translation="MYLERPELYKQSEINFWDDEYISEQLLKAHLDTNFEGASRNFNF
                     IEDSVNWIVTVANPVNYPKLLDLGCGPGLYAEKFAQKGYKVTGIDFSKRSINYAQNRN
                     KETNLNINYLFQSYLNMNYNEEFDLATLIYCDYGALSTENRRLLMEKIYDSLKPGGKL
                     ILDVFTINKYNNFEEIKYWEINEDGGFWSNEKYMCLQDNCKYNDYNTLEQTLVITEKD
                     ENVFYVWNHYFSKESFLLEVENIGFKSVEFFSNVKGEAYSDDSMTMGLVLQK"
     gene            39137..39898
                     /locus_tag="PC414_001368"
     CDS             39137..39898
                     /locus_tag="PC414_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896901.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HDF2463770.1"
                     /translation="MIGIYFSGTGNSRYCVEKFLQEYDITANSYSIEDNELLQHINNH
                     ENIIFSYPVQYSNVPKILKDFIINHSTLWNGKRVFIIATMALFSGDGSGVLARILKKY
                     GAITVGGLHVKMPDSIGDEKVLKHSLEYNRKLVIKAEKKVTKAAKKLKHGSPPKEGLG
                     FLSHIVGLFGQRLYFINKTNMYTDKLKINTEECIVCGKCVNLCPMKNLVIKNCMIVAN
                     GQCTMCYRCVNNCAKKAITLLGKKVVEQSNIKKYM"
     gene            39963..40745
                     /locus_tag="PC414_001369"
     CDS             39963..40745
                     /locus_tag="PC414_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HDF2463771.1"
                     /translation="MEYYIVDSFATKLFKGNPAGVCVLDRRIPLELMQKIAEENNLPE
                     TAFVVKCKGNYELRWFTPKAEIDLCGHATLAAAYVISNFIDVNVKRIDFFTQSGKLEV
                     TRNGNLYEMIFPEIMPIEIELSPQQANLIGCVPSAVYSSRDLILLLNSEQEVINYKPN
                     YAQLRKLTDWLGIIITAQGSNTDFVSRYFCPELDSEDPVTGSSHCNLIPYWSEKLGKH
                     KMVAAQLSNRGGIIQCEVLKDNTVKISGEAVLFMQGTIKIDI"
     gene            41012..41431
                     /locus_tag="PC414_001370"
     CDS             41012..41431
                     /locus_tag="PC414_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjdF family protein"
                     /protein_id="HDF2463772.1"
                     /translation="MDKISGKLTVLFEEPFWVGIFERQDGKKYEACRVVFGAEPKEVE
                     VYEFILERFFSLDFGSIKLEKNVTKDNIGYKRMQRKVKKEQEKETIGTKAQNALKLQY
                     EERKQDRKNLAKNRKEEEKERLFNLKQEKRKAKHKGH"
     gene            complement(41534..41800)
                     /locus_tag="PC414_001371"
     CDS             complement(41534..41800)
                     /locus_tag="PC414_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HDF2463773.1"
                     /translation="MKNFILIYFIVINSIAFFSMYIDKKRAIRNEWRIKEATLMSIAV
                     IGGSIGSIIGMYSFRHKTKHIKFTFGIPFILFLQFLLVYFYILK"
     gene            42027..42728
                     /locus_tag="PC414_001372"
     CDS             42027..42728
                     /locus_tag="PC414_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463774.1"
                     /translation="MKFIVEKEVFDKLENVCFGVVVAKGIDNTKEIERISNLLDISID
                     RVEDYFKDKKVKESEEIIPYREAFRSLGMNPNKFMSSIEAMTTRVAKNKKLPHINPIV
                     DLGNSISLKYLLPMGAHDMDFRNDDVYVRFSKKGDKFVPFGETDVELMEEGELIYSVG
                     DMVKTRRWIWRQGEEGKITNSSKNIFFPIDGFTDANLDKVMSAREELAKLLKEIFNCE
                     IKVGFVDKDNPEMEI"
     gene            42810..43418
                     /locus_tag="PC414_001373"
     CDS             42810..43418
                     /locus_tag="PC414_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDF2463775.1"
                     /translation="MKKTIIKNVNPMIENKFIGLFEIEYKNKLDEDKVWMVASRKSSE
                     QLKNIYLKNEEDSVDAVVIVGLHKSSKKLILIRQFRVPINGYIYELPAGLVDEGESID
                     ISVERELREETGLTLLEIKKNKSIDKVYLSPGMSDESVAFVYCICDGDITDEFLEPDE
                     EIEALLISQEDAKEILQSNHKIDTKAFLILQMFVSLGAKLFE"
     gene            43742..44206
                     /locus_tag="PC414_001374"
     CDS             43742..44206
                     /locus_tag="PC414_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4624 family lipoprotein"
                     /protein_id="HDF2463776.1"
                     /translation="MKKLVIILISIISMVSLTACKKSNESKYNNLNNKESGQTTIEMD
                     LDKNYDTSDPFVNARLFCVSNDIDVLDTEISFKMDGDSGIVEIKDNKTYETLWSNTWH
                     GSVDNDTFTISLSNIQKEKEYAICFTGIKINHAVVKVTFESNLVKEKEKPSK"
BASE COUNT        17750 a         4581 c         7160 g        14959 t
ORIGIN      
        1 taaatacata taaagatatt tttaagaata aaatttatta ttaaataaaa aataaaggag
       61 agtgatttaa tagcatggca tcaaaacaaa taccaagaaa caatttagaa aagtttttaa
      121 gaataagagt cttatcacat acaggcgata aaatacatat aaagttacct gtaaattttg
      181 caaagagaat gattgagaat aatgctttgg atttatttaa tggaaaagat gatgtagtag
      241 atggtaaaaa agttactgaa atattagtta aggcatttga ctatgattta actggagaag
      301 tagttcactt ggagagaaaa aatggagaca ttattaaagt ttcaattgta caatagaatt
      361 attttatatg aattttattt ttgaatagtt ataaataatt agaaatttgt aaaaaagtat
      421 aatatattat tagtaatggt acagaataca catttataat ttatagatgt gtattttttg
      481 tttaaaaaat ttaaatatat aaaactataa aggttacaat tttggaaata gatgtaaata
      541 tatgttgtat tgtataataa gagtaatata aaatctagta aaaataatca aataaattta
      601 gcttatgaga gggataacta tggaaaataa ggattttaaa ttaacaatat gtatatttgc
      661 ttttgtaatt ggagtagtag tattattctg ttgtataggt ttaggtgaat ttagtgcaga
      721 gcatattatt caacaaaatg gtggaatcat ggatactgac caatataata tttatctaga
      781 ccaatctatt tctcaatata gaaattttgg ttctatatta gctctgttag gaggaatagg
      841 gatattgttt gataaatata tcagaaaata ctagagatta tgttttgtct aaaataaatt
      901 cttatgagga gataaaaaat ggcaacagaa atatttactg taaaaattac acccttagag
      961 gcacaaaaaa tgataaaaga aaatcttgat gcagatttag tttttagtga ttcttataat
     1021 ttaggagatg ataaatttat actaattaca acttttgaga agtattatgc aagaaataat
     1081 agtgatgcag gattaatagt agtttatgaa aataccacag gaaaaacagt tattaaagct
     1141 acttcaacag gcagtgcaat tggaatttta aaaattgatt ggggtgcagg tggaaattta
     1201 cttaaaagag taaaaaacat acttgctgat tatattattt aattatttta tctaactgat
     1261 aatatgtcaa gaaaatctcg taaaagaggt taagaacatt tttataatat atagataaat
     1321 aagaatttga aggtaaagtt tttaaggtat aaagaaaaat aatataataa aacgaattgg
     1381 ctttataatt ctttataagg caaagagcac atttctttat ataattgttg gatattaaat
     1441 aatatgtaaa tacaccattt acatataaaa gtttaattaa tatgttaaga gaaaatgaaa
     1501 tggaaattac taaaaaatat ttttaaattg gataatggtt gatattatgt ttatacaaaa
     1561 atcatgtaga attttatata ttttaaatat gatagtttaa aattatgaaa gaatgataaa
     1621 ttccagtttg tagggtaatt ttgataaata atattaataa gtatgagtaa aaagtaggag
     1681 catattaata taatgtccct acttttttat tattaaaatt gtttataata tcaacactac
     1741 attaaaacac aacctaaatt tgaaaaacaa atagtttaga aatatgtact tttttatcta
     1801 ttatattctt ttaaatcgaa ttttttcatc tgaacataaa gtctcaaatc tatatgcgtc
     1861 tgtcatatca taaaaaccaa aaaatataaa gccatcacga gaagtaaatt catattgtat
     1921 attttcaatt ttcaacatgt tttctacttc gcttattttt tcgctatata taatttctac
     1981 catataattt atcattgtag aatccctcct attaatatga aaatatccaa atatataata
     2041 acatatttag atgatataat agtatccaaa taaaaaataa tattattaca ttattgaatg
     2101 aagaattaaa atgaagataa ataaaacaaa tattggttat aattgattaa aaaatagtta
     2161 tgtatacttg tgactttatt tatgggcagt tcaagtttta tgaaagaagt catatatata
     2221 tggaatattc taggcagaaa ctgtttaata ataagagaat actataaaaa tttgagtatt
     2281 acataaaaaa taagaagcca taaatgatat aagcattatc tatagcttct tacttctcat
     2341 tgattaagat tatatataaa aataatcact atattttttg tttaagtttt agtacttgtt
     2401 ttactatatt tgttgagtgg tcagaaattc tttctagatt agtaagtaag tcaagataaa
     2461 gtacaccagc atcaattgta caacttccat tatttagtct aatcatatgt gaatttctaa
     2521 aagttttttc taaataattt acttcatttt caacttctaa aactttattt gctaactcaa
     2581 aatctttaac atctaaaact attaaggcat ccttaaaatt agtaattgtc ttattgtata
     2641 tttcgtatat ttctcttatc gcactatcag aaaaactaat gttcatttcc ttaacactca
     2701 tggccaattc agctatattt tctgcatggt cagagactct ttcaatgtca tttacagtat
     2761 taaaaagcaa atctgttcta aagcgttcat catctctaag aggttcttta gaaagtttta
     2821 acaaatagtt caatatatct ttctgtaact tattgacaac tttttctgac tcaaaagctt
     2881 tattaatatc ttcattgctc ttatttaaaa atcctgcaat tgaatgttcc aaagagtttg
     2941 tagctttatg acccattctt acaacttctt gtacagtatt accaagtgct atagatggag
     3001 tgctaaacat cctttcatct aagtacttag tattatttac tatttctgat tcagtttctt
     3061 taataggtat aagtctattt gctaatttaa ctatgtattt agaaaatggt aataatatta
     3121 taacatttgt aagattaaat aaagtatgtg catttgctat ttgtctagct gtatcagttg
     3181 ggtcaaaatg agtaactatg gctgatatag gtttatttaa aacaagcata aatattattg
     3241 taccaattac attaaaactt aaatgcatta cagctgcacg tcgagcattt ctacttgcac
     3301 caacacttga taaaagtgaa gtaacacaag tacctatatt atctccataa agaataggta
     3361 aagcagatga tagaggaagt attccttgag aagctagtgc tattaacata cccatagatg
     3421 cacttgaact ttgaacaata ccagttatgg caaatcctgc caatatacct aatacaggat
     3481 ttttactaaa atataaaagt gcatcagtga atgctttata ttcagcaagt ggagctactg
     3541 catctttcat aaactccatt cctgtaaata atataccgaa acctataagt atctcagata
     3601 aatgctttgt tttttgattt gatgtaaata aatagaataa aatacctata cctaaagcaa
     3661 ttggtgcaat tccttcaaga ttaaatgaaa ctaattgagc agtaactgta gtacctatat
     3721 ttgcccccat tattacacca atagcttgac ttagattcat tatacctgca tttacaaatc
     3781 ctacaaccat aacagtagta gcacttgaac tttgtataat acctgtaacg actgtaccta
     3841 ccagtacacc cataacaaca ttacttgtta agagttcaat aatttttttt aatttgtctc
     3901 ctgctgattt ttgtaaacct tctcccatta aactcattcc gtaaagaaaa agtccaagtc
     3961 caccgataag gctaattact atattcacga tatttcctcc cttaagaaat taataatttt
     4021 tttatctgaa ttataaactt tattaactaa aatttattgt ttccataata tagaataaca
     4081 aattttgtca ataaaaatat taatgttttg ttaagtatac gttaagagaa tgttaaattt
     4141 ttaagttgtt gttaatttta gaaaatttat gatatatagc aatttaaata cttgaactta
     4201 gatttttagt attttatgta caatcaattt aacaaagaat taactataat tgtaaaaaat
     4261 atgtaaaaaa ataacatcct tttagaatga tattttttag ttataaatat ttttatattc
     4321 aattggtgta aagttattaa actttttaaa aattatacta tagtaatttt gactattgaa
     4381 tccaattagt acagcaacgt ctaaaataga caagtctttt tttaatatat actttttgct
     4441 tttttcaact ctaactttac ttaaaaaatt agtaaaagta tatccacttt ttttcttaaa
     4501 taaagaaaaa aacaactttt attaagttgt aattcattac atatattact tactgttata
     4561 tcttctgagt aatttttgtg aatataatct atagttttct ttaagttttc tttacataaa
     4621 actaagagtt ggtctatgtt tcattttatc aaggcatatt tattaaaaaa tcaatctcta
     4681 tttgttaaaa taatttatga atgatgttag aaactgtaat atattggata taatataata
     4741 aacagataaa ctctgtatta agaggatata tagtttatct taattatagg ggaagtgagg
     4801 atatattatg gaaagattaa tggaactcat agaaagaaaa tttttaaagg atgtagaact
     4861 tttagaatta gaagacatag aaaatgtaat agaatgcaaa atgacacagc catataatgc
     4921 tcattataat gaatatttag taaaagtaaa aaatggagat gaagtagaag aatattttgt
     4981 gtatttaaaa catagataaa cataaaagag gtttatattt atttgtatga ttataaaata
     5041 aatacaagcc tctttttttg ataaatataa aattataaaa tttttattta tttaattttt
     5101 tcaacaacat tagtcaatat tgaatctaaa tcatcactaa tttctgtaac tccattatga
     5161 gtaatatcta taatttcttt agtcatagga agttctccta aaagctctaa gtctaaatca
     5221 tctaaaaact tttcagtaga ttctccttca aataacttga tttttttaga acaatcagga
     5281 cattggatat aactcatatt ttcaataact ccaaggacat ttatgttcat ttttttagcc
     5341 atattaacag cttttgatac aatcatagat accaaatctt gaggtactga aaccataact
     5401 attccactta taggaataga ttgcataact gttaaggcaa catctccagt accaggaggc
     5461 atatctatta ataaatagtc taattcttcc caaagaacat cagtataaaa ttgctttaca
     5521 acccctccaa gtaaaggtcc tctccaaaca acaggttcat tttcatcatc tatcataagg
     5581 tttatagaca ttacttttat gttatttgag ttttctacag ggtaaatata agagccatca
     5641 gaataagctt ttacattttt tacacccata agtctaggaa tacttggtcc agttatatct
     5701 gagtctaata taccaacctt atatcccatt ttgtttagct tattagcaag taaagcagtt
     5761 acagttgatt ttccaactcc acccttccca ctcataacac caattatttt tccaaattta
     5821 ttgttagggt tattttcaat agaacaattt gattgactat tacaatttcc ctttgatgga
     5881 catgaattac aatctgccat atctattacc tccaaacttt aatatttatt agattatttg
     5941 taacctacat atactatttt agtacaattg aaccataaag tcattattta aaagcaactt
     6001 agaagatttt ctattgattt attcatagac tatagcaaaa agaatacaat aaaaaaatta
     6061 taacattctt gaaaaagtga ttatttcaat ttagttatag aacttagtca tataaatata
     6121 tagaaaatat tttaatatgt tatacataac gggttatttt ataagtttaa ctataaatta
     6181 ggtttatatt ttataaaaat ttgctaaaaa aaacaattaa aaattgtaaa gaaaaaagat
     6241 ataattagaa ctgtagtaat aaatgtttta ttatagataa tagagtttta agtatcttaa
     6301 ataatatttg gaggaatatg aatggaatta agaccaagaa gtgcaaaatt agaagaattt
     6361 gataaagtaa tagaattaat aaactatgta tttagaatat caagaaatca taaaccaact
     6421 atgatggaag aatttcctct tttactaagt aaaaataata tagaaaatat gattataata
     6481 tcagaagatg ataaagtgat ttctgatgta aactatctaa ttcaggatgt atctattcaa
     6541 ggaaacagat tgaaggttgc agctataggt ggagtatgta ctcatccaga ttatgaaaag
     6601 agaggctatt catctaagat attggataaa gtagaagaaa aaatgtttta tgatggtgta
     6661 gatattgtaa ttatatcagg aacaagaagt ctatacagta gaagaaattg tagtttggta
     6721 aagagttttt ataaatatac aataaaacct gaagatgtaa aaatagcata tgaaatagtt
     6781 gaatttgatg aaactaattt tgaaaaagat aatgatttag ataaaatgat tgaactatat
     6841 aatcaaaata gtactagatt tataagaacg agagatgagt ttcaaaaact attgcatgca
     6901 gcaactattg cctggggacc aattggttac aagaaggtat ttataaagga aaataataat
     6961 ataataggat atttgataat aagaacaata aaaaaagaag attctactgt aggagaagtt
     7021 gctgaaatag ggttaaactc tgtaaatgtt gaaaatatat tgaaatatgt tgcaaataaa
     7081 tttggattag aatatttaaa ttataaagta catgtgaaaa atcttaaaga ccagttgaaa
     7141 tgtaatggga caaaatcttt ggattatcaa caaggaacta tgaaaataat aaattttact
     7201 aagttgtgtg atagtttaag aagttatttt agtcaatatg tagattttga attactaaaa
     7261 tacatggaat ttaaacaagt tgaaaataaa tatattatta aatacaaaga agaagaactt
     7321 gtaatagaaa atttggataa gcttaataaa ctgttttttg aaaaaaatga ggaacagtac
     7381 aatgaattta aacatctaaa aaatatatat gaatttgcta caaaggcatt tcctgtagat
     7441 tttccatgga ctgcaaattt aaattatcaa tagttttaga tgatattaaa taaaattgat
     7501 tgatacctag agttagtcct tgttaaataa gagaaattat tggatattga aaaagcaatt
     7561 ttgtaagtaa ttagaaagga tgagtattat atggacatac ttgatgatat agcagaatgt
     7621 atattaaata tgggtgtaga taatatagag aatttagtaa aaattgctat agataaaaat
     7681 attgatgtgg aagatattta tgaatatggt ttaaacaaag gtatgatagg agctttagat
     7741 aagtttgaga ataaggaata ctatctttca gaagttatag tatgcacaga tgcattaaat
     7801 aaaggtatta atcttttgaa gggaactgga aaagtaaaga aaaaatcaaa gggagtaatt
     7861 ctgatgtctg tagtagaagg agatactcat gagataggaa aaaatattgt aaaagtaatg
     7921 gtagaagcca ctggatataa ggttattgac ttaggtgtaa atagaaaaag tgaagatata
     7981 atagaagaag ctataaaaaa caatgtagat ataataggct tatcttcaat gatgacaact
     8041 acaatggaaa atatgaaatc ggttatagac gaacttaata tgattgagat agataaaaga
     8101 ccaaaagtaa taattggtgg aggacctgta agtatggagt ttgcagaaga aataggagca
     8161 gatggataca gttcaaatgc tccaaaagca gttaaattaa taaataaact tataggaggt
     8221 gaagtttaaa tgttttcaga tatgacttta cttcagtata taaatagtga agatagaggt
     8281 ttttttctgc ctgatatggg tacaaatgga ttgttcttaa taaataaaaa ggcatatgaa
     8341 gtatatgata gtgtagattt gcaattagaa ttagcaaaga ctatggacaa gtattttgaa
     8401 tcagatttta tatactcatt ctgtgatggg attacatttt gtgaaactct gggtttagag
     8461 acattaagac cagattatga ttttccaagt gtattgacac atactataac tgacagagat
     8521 aaattaagaa aattagatgt tccagaccct tatacgagtg gacgtatgcc actcaatata
     8581 gagagcttat cagttatatc aaaaagtatt aaaaaaccac tatatgaatc gatacaaggt
     8641 ccatttacac ttgcaggtca attagctgga gctactcaac ttttaagatg tataataact
     8701 gataaaaaat ttgtagaaga attattggaa tttacaactg aattggtaag aagatatgct
     8761 gtagctgcaa ataaagcagg agcaaaatat atttcaatag cagagccaac atcagtaacc
     8821 ctaagtaaag atagatttga tgaatttata gtaaaaaatt taaataaaat ctacaatgaa
     8881 cttgattgtt ggaaaggtat gcatatatgt ggagacacca gagaattatt agataatatg
     8941 ctaagttgta atatagatgc agtaagttta gaccaaattt tagactatga agaaattgct
     9001 ccaatgattc ctagagatat agttttgatt ggtaatttag acccaataaa acttcttgga
     9061 cgttctaagc cagataagat tagaagagag acgctaaaat tgttaaagaa gatgagaaga
     9121 tatgataact ttttatgtga ttttggctgt aactgcttaa ataccactcc agtagaaaat
     9181 ttacaagcag ctataaaggc aggaagaatt agttataaag aattagatag gattgatatt
     9241 gatgaattat aatagtaata aaaataaata tcttttaaag gatataaatt tagatgatat
     9301 ttttatagat aagaatattg tgctaaagtt tttggggtat ggaaatagaa aagctccaga
     9361 tacaattagt aaaatcgttg aaagagaaat aagaaatata aatgatattt tagatatcaa
     9421 gatatatata agtgaagttg atattaataa aacacctaaa gaatgtaaaa aagcatttgc
     9481 tgtactatat acaatagggg ataaaattga tattaagatg aatgactata tggaaaattg
     9541 caatatgatg gcagggctag cattggataa aataggtata gtatgtttag attatattaa
     9601 tgagaaaata aaaatgtatt taaaaggaaa gtatgatagt ctcaaaatat cgcatgaaat
     9661 atatccagga gataaagatt ttgaagtaga gagacaaaag gatatttata actatgtaaa
     9721 aaataaatat aatagtatag aaatagaaat taatgattat catcaactat cccccataaa
     9781 aagtgttgca atgcttattt taatggggga tgaagaaaat ttagaatcaa gatgtagtaa
     9841 gtgtcctaga aaatgctttt agttaatttt caagactaag atttgttttg tgtataaatt
     9901 gcaataattt ttttggtcat tttatgtata aactaaaaaa attattgcaa agtaatattc
     9961 tatttaattt gaacagactt acaaactata gtagtttctt taattcaata agtgtattta
    10021 tttcataagt tggtatatga ttagtataat ttatactgtt attggaattt aaccaacaag
    10081 tatctattcc tgaatttatt ccacctaaga catcagaggt taagctatcc ccaattatta
    10141 aggcagaaga tttatcatga caattaaact ttttgagagc gtatttaaat atttcaggat
    10201 ttggttttga tattttcaac tcttcagata caaccattcc atcaatatat ccttttattt
    10261 ttgaattttc aagtctttta ttttgaactg cagaaattcc atttgttatt ataacaatag
    10321 tatacttttt tttaagatat tgtaatatat cgattgcacc tggaattaaa aatgtacatt
    10381 cacctaaaaa atttaagtac attttgctaa gagtttcaga atcaacatcc aaagatattt
    10441 tatttgcaaa aagttcaaat ctaagtaatt taagttcatt caaagttata atattttttt
    10501 caaggtcgag ccaaagctta tcacttatat ttctatatga ctctatataa ttctcaaaat
    10561 taaattctaa atcaaactca gatagaagtt ttttaaaagc atgtgattca cttttcttaa
    10621 aatcaaataa agtatcatct gcatcaaaaa atataaattt atatgtcttc atggttttta
    10681 ctcctttaca agatgactgc aaaaattact ttactttcac tatttgagtc attttgccat
    10741 ttatgttttg ttcctaaagg aacttttaca ctatctcctt tggacaaagt aaattcatca
    10801 tcatccataa aaagtttaac ttctccctcc aaaacatatg caatttcatc tccattatga
    10861 gtaatcctat caacacaaga agaagtgttt ggagctaaat ccataattgc aaattctaaa
    10921 ttaccactag agtctggaat taaaatctca tatataactt ctttgcttcc aggtaaaata
    10981 actttttttc tattatcatg tcttactatc aagttttttt tatctacttc agatacaaaa
    11041 aatgtaaata aaggaacatt tagagaagag gctatagatt ttaatgaatt tacagatgga
    11101 ttagcaagac ctcgttctat ttggctcaat aatgatggtg taacatcagc tagttttgct
    11161 aaatctcgaa tacttaagtt ttgttttttt cgaacttcag ctattttttc ccctaaattt
    11221 aaatcattca tataaatatc ctctcttttt aatttaaaaa atattaactt aaaataatca
    11281 agtaaaatga ttatgtagta tgaaattatt ataacatata gaattaggtt ttgaaaaaac
    11341 tatgttcaat ataattaaat aaattaaaat ataattgaca aagtggatac agaatactta
    11401 aaatatattt aaataaaatt aagcaaaaga tagttaattt atgataggag gggatattat
    11461 gcaaataacg acttttggag ctatatttgg tttattaata gcaattattt taattataaa
    11521 aaaatttcaa gctgtatata gtttaatgtt aggagcattt attggaggat tagttggagg
    11581 agcaaatata acacaaactg tagattttat ggcaaatgga gctatgaata tatcaccatc
    11641 aattcttaga gctttggcat caggtgtatt agctggaagt ttaataaaaa caggggcagt
    11701 agataaaata tcagaacaga ttgtaaaaat atttggtgaa aagagggctt tattttcgat
    11761 tgccatttca actatggtac ttgctggagt aggtgtaaat ttggatgtat ctataataac
    11821 tgtagcacct ataggattat atataggtag aaaattaaat tattctaaat tgtcaatact
    11881 attagctatg ttgggagggg gtaaagcagg taatataata tcaccaaatc caaatactat
    11941 agcagtagca gataattttt ctgtcaatct ctctagtgtt atgatggcaa atataattcc
    12001 agcaattatt ggagttgtta ttacagttat tttagcaagt atattaataa ataaaggaaa
    12061 taaagtacaa tcatatgaaa ttttagagca aagagaagat ttaccaagtt tatttaaatc
    12121 attatgtggt ccaattatag ctatatttct attgttttta ggaaatgtat ctcctatagt
    12181 tatagaccca atgatagccc ttccaatagg aggaatcgta actctaattg tcacaggaaa
    12241 cttgaacaat tcaagagaat atttaacttt tggattatct aagatgcaag gggtatgcat
    12301 attattactt ggaacaggga ctatcgcagg aattatacag atgtctgaat tacaacagag
    12361 tacaataggt gctttacaat ttttaaatat gccacaattt ttattggcac cagtttcggg
    12421 tatacttatg tcattagcaa cagcatcatc aacagctggt gctactatag catcatctac
    12481 ttttcatgat gctataataa atggtgggct atcaccgatt tcaggtgcat caatagtaaa
    12541 tgctgggtct agtgtattcg agcaattacc acatggctca ctatttcata ctagtgcagg
    12601 tagtataaac atggatatag gtgaaagatt taagttaatt ccatatgaag cattgatagg
    12661 aatagtaatg actataattt caacatctat tcaattagtt ttataataaa tataaatatt
    12721 taatgaaaaa agtaggattt tgttattaaa tattgaactt attatagtaa aaaagaagat
    12781 tggagtttta aagtgaataa aaaaaagatt gttattatag ggattattta ttcattttta
    12841 gtagtatttt cacttacaaa tatgtatgta aatatggagt ataatctaaa tgtatttgaa
    12901 tatattaaga agtcacttcc atttacagaa gaagaaaaaa agtggttaga aaaacacaag
    12961 aatttgattt acagctctga ccaaagttca cctcctttga gatataaagg taaggaagat
    13021 ggacagtata agggaattat tgttgattta ataaattctc tgtcaattca aattggtaga
    13081 gatttttatt ttaaaccaaa taattggtgg aaagaatctt tcgtaaattc aatagatgat
    13141 agtataaagt tttttgactt gattccatca aaagaaaggg caaataaatt tatatttact
    13201 gacccaatat acactttgag tgcaaatata ttgaaagata aaaagtccca agatataaat
    13261 agttacatgg atttaaaggg gaaaacagta gcaattccag aaggtgatta ttctataaat
    13321 tttttaaagc aaaaaattca agatataaac attttattga ctccagatat aaagactgga
    13381 gtcaaccatt taatgtctgg aaaagtagac gcagttgttg gtgatgaacc agtattgcga
    13441 tactatataa ataattatgg actttcaaat aaatacagtg ttttaagtaa tcctatatat
    13501 actaaaaaag cagttcttgc tgtacctaaa caatatgaag agctggttag tattttaaat
    13561 aaaggcattt ttaaacttca gaaaaatgga gtgtataaag atttaaagaa aaaatggtat
    13621 tctacgtata atgaagttga tgatatattg tatgagcgag gaattgtgcc tagtatttat
    13681 ctttttatag gtataatttt aatatcaatt tatgtttttt atagctatac atatttgtta
    13741 aagatagaaa tcaaaaggaa aacagaacag gtaatagaaa ataaaaaaac tttagaggca
    13801 acttttaata gtattacaga tataataatg cttgtagatg aaaataacaa tatagtagaa
    13861 tcgaacaaag tactatatga ctttatgggt gaaatgagtt ataagatagc agatttgatt
    13921 agcatgataa aaggtgttat tgaaaatacc ttctctgaaa atactaataa aactagtgaa
    13981 atagaaatac acaataaaat tttaaagata aacacttttc ctgtagagta taaaaaaaat
    14041 aatactgagt atatagtagt tcttattaaa gatataacta atgataaaat agtagaggcg
    14101 aaattactta gagaaaacaa aatgatatct atagggcaat tagcttctgg ggtagcacat
    14161 gagataagaa atcctttggg tataataaga aataattgct atctcttaaa agataacgtt
    14221 acaatggaag aagtaaatga ttgtgttaaa tcaatagaaa gtaatgtaga tagggcaagt
    14281 aatataataa ccaacttgtt aaatttcgca agaatatcag atgataattt agaacatata
    14341 aatataaaaa attttattga aaatatagtg aaacttcaat ataaaatgtt acagttaaag
    14401 aatgttgaaa taaaaattga ctgtgaacat aatttaatat gctatataaa tggagaatcg
    14461 ttaaaacatg tatttataaa tttaatttct aattcaatag atgcaataca tcaagatgga
    14521 aaaattatta tatactgtta tgaaaaaaat cattgtttat ttatagattt taaagataat
    14581 ggtgaaggaa taaaagaaga tgtcctaaag gacatattta atcccttcta tacaacaaaa
    14641 ccaattggtg agggaactgg tttaggttta tatattactt ataatgagat taaaaagaat
    14701 aatggagata taagtgttga aagtaaattg ggggttggaa cttgttttca tataaaaatt
    14761 ccattaaata aagaggtgac tatataatga aaatactatt agttgatgat gagctagaat
    14821 atggagtggt aatgaaaaaa attctacaaa agaagggcta tcttgtagat atagcactaa
    14881 gtggtgaaga agctataaat ataattaaaa aagataaaaa ttatgatttg gttttatctg
    14941 atgttatgat gaaaaatatg gatggagtac agctattaga tagaattaaa tctattaata
    15001 gagatataga ggttatatta gttacaggat atggaagtat tgaaaatgca gttgatgcta
    15061 tgaaaagagg agctttatct tattttataa aaagtaatcc cattgaaaat ttattagaag
    15121 aagttgaaaa ggtaaaggca agtaaaatat ctgtttctgt acaaaaaaat aattttgaat
    15181 ttactttgga atctaaaaat agagatttta ataatatcat aaaaatagca aaaaaagctg
    15241 cttgtaaaga tgttaatata ttaatcttgg gggaatctgg tgtaggaaaa gatatattag
    15301 ctagatatat acatagcata agtccaagaa agaatgagat atttgtacct gtaaattgtt
    15361 gttctttttc agaaaatcta ttggagtctg agctgtttgg tcatgaaaaa ggttctttta
    15421 caggagcagt agatagtaga aaaggtagat ttgaactttc gaataaagga actctatttt
    15481 tggatgaaat aggagatata cctctaaatg ttcaggtaaa acttttgagg acacttgagg
    15541 acaaatctat agagaggata ggaagtaata aatctattaa agttgacttt aggttaattt
    15601 gtgctatgaa taaagaacca aaagtggaaa tatcaaatgg aaatataaga gaagattttt
    15661 tttatagaat aagtacaata acaataacta taccaccact aagaaagaga agagaagact
    15721 taactactct tatagaattt ttcttaaata agtaccaaat tgaacatgat aaaaagatac
    15781 actcgattga taaggaagta aaggattttt tattaaatta caattatcca ggaaatataa
    15841 gagaattaaa aaatataata aatagacttg tagttttaag tgaagaaggt aatttatcaa
    15901 aagacaattt aaatttaata tcaaataatg tatacataga tgataaaata agtattagac
    15961 ctttaagaga aataagaaaa gagtttgaat gtgagtatat tgaaaaagtt ttatctttgt
    16021 gtggaaataa tatttcaaat acagctaaaa aattggagat tagtagaaga cagttaacca
    16081 ataaaatatc agagtataat attaagtgag aaaaaaactt ctcattaagt gagaaaaaaa
    16141 tttctcaaac tgtattaaaa gataaacaga aaaaatctgt ttatcttttt ttattataat
    16201 tttaaaaatt aatatttaca ataattattg ctaaattcaa tattggtgta aattaaaaat
    16261 gaaaatttct atgacattaa tacatagaat atatatttta aataattggc atagaaaatg
    16321 ctttatatat taataaacgt aataaatact tttaattgcg attaaatata ttaaggggga
    16381 ttttcatatg aagctagagc tagggaatat atttataaaa aatgttgaat ttggagaaaa
    16441 gacagaagta aaagatggag tactatatat tagtagcgaa gaaatagaaa agatagtatt
    16501 agaagatgaa aggattgtaa gtgtaaatgt tgaacttgca agacctggcg aatcaataag
    16561 aatagctcca gttaaagatg taatagagcc tagagttaaa attggagatg aaagcaaaat
    16621 atttcctgga attataaata aagtgaagac tgtaggaagt ggaagaaccc atgtactttt
    16681 aggagcttgt gttgtaactt gtggaaatat agttggattt caagaaggcg taattgatat
    16741 gagtggtcct actgctaaat atacaccttt ttcaaaaaca aataatatat gtattgttat
    16801 aaaagcaaag gatggtatag atactcatga ttatgaagaa gcagctagaa ttgcaggatt
    16861 aaaaataggt gcatatgtag gtgaggcagg aagagaagtt gagccagatg aagtcattgt
    16921 atatgagaca aagccattat taaaacaagt aaaagaatat ccagacctgc caacagtagc
    16981 atatgttcac atgttacaat cacaaggact tttacatgac acttattact atggggtgga
    17041 tgctaaacag atagtgccta catttatgta tccaactgaa ataatggatg gagctataat
    17101 atctggaaac tgtgtagcac catgtgataa ggttacaaca ttccatcatt taaataatcc
    17161 agttatacat gacttatata aaagacatgg aaaagattta aactttatag gagttatttt
    17221 gactaatgag aacgtattct tagtcgataa agagagaagc tctgatatgg tagctaaatt
    17281 aatagaattt ttaggagttg atggagttct agtgacagaa gaaggttatg ggaatccaga
    17341 tactgaccta atgatgaatt gtagaaaatg tagtgaagtt ggagcaaatg tagttttgat
    17401 aacggatgaa tttcctggaa aagatggcaa gagtcaatct atagcagacg ctactaagga
    17461 agcagatgca gttgtttctt gtggtcaagg aaatctagtg gttcatttcc cagcaatgga
    17521 aaaaattata ggaactttag attatgttga aatgatgata ggtgggtata aaggatgctt
    17581 aaatgaagac ggaagcatgg atgcagagct acaaattata atagcatcaa ctatagcaaa
    17641 tggctataat catttgacag caagatatta ttaaataagt atttgaatat aaagttctgt
    17701 tgatgtaatc attggtctaa ctgatgtaat aactataaag tgatttggag gattaagaga
    17761 tgagtaaaat cagagtggtt cattatataa acaatttctt tgctggtata ggtggagagg
    17821 aaaaagcaga tattccgcca gaaaaaaggg ctggtgtagt aggacctgga atagcatttc
    17881 agagtcaatt taatgatgat gcagagataa tctcaactgt aatttgcgga gatacgtatt
    17941 ttggtgaaaa tattgagagt gcaactcaag aacttttaaa aatgataaaa gaagaaagcc
    18001 ctgatttatt tattgcaggc ccagcattta atgcaggtag atatggagta gcttgtggga
    18061 ctatttgtaa ggcagttgaa gaagagttaa atattcctgt aattacaggc atgtataaag
    18121 aaaatccagg tgtagatatg tttaaattag atttgcatat agtgtctaca ggaaattcag
    18181 cagcaagtct tagaaaggta gttccaataa tgacgaaact aggtcttaaa ttggttagag
    18241 gtgaggaaat aggacctcct gaagaggaag gatatataat gagaggaata agaaaaaatt
    18301 tcttccatga acttagaggg tctgaaagag caattgatat gttggttaaa aagatgaaag
    18361 gggaaagctt tgaaactgag tatccaatgc ctgaatttga tagagttaca cctatgaaac
    18421 ctgttaaaga tttatctaag ataaaacttg ccctagtaac ttctggaggg atagttccaa
    18481 ttgataatcc agacaaaatt gaatcttcaa gtgctactaa atatggaatt tacgatttaa
    18541 cagatatgga ttctatgtca aataaagaat ttacaactat acatgggggg tatgacagag
    18601 catatgtcct tgaaaatcca aatttagttg taccactaga tgtagttaga gagcttgaaa
    18661 aagaaggtgt aattggagaa ttagtagatt attttataac tactacagga acaggaacga
    18721 gtgttggcaa ttctaagaga tttggagaag agttttctaa gaaattgtta gaagatgatg
    18781 tagatgctgt aattttaact tctacatgag gtacttgtac tcgttgtgga gcaacgatgg
    18841 taaaagagat tgagagagct ggtatagcag ttgttcatat atgtacagta gtaccaatat
    18901 cacttacaat aggagctaat agaattgttc cagcagtagg gataccatat ccacttggaa
    18961 atcctatgtt aggagaagag gaaagtaaaa aaattagaaa gaatatagtt ttaaaagctt
    19021 taaatgcact aacagaagat atagaagaac aaacagtatt tgaataataa aaatacaaca
    19081 caacactgaa ataaaaaaag atatcaaaaa ataatttttt aaaattattt tacgatatct
    19141 ttttttatta ggtaaattcg aaatttgcat ttttttaaat taaatttata aaagtgataa
    19201 attagtttat tataatattg atacacaaat tcaaattaga tatactatga aaagcgagat
    19261 tattacattt tggaacaaat attcaacata ttagatcata ttaattgaac agaaaaaatt
    19321 aactttatat aataattaga ataatgataa gaaagaaggt gtatatattg atttcaattg
    19381 ccatttgtga tgatgaatcc tatatgctaa atcatttaaa ggaaaatata gaattataca
    19441 tgaagcagaa gaatttatta tataatatag agctttttaa taaagtagaa gatttactat
    19501 gcaatcatga aaagtttaat attgtatttt tagatataca gatggaagag attaacggaa
    19561 tgcaggcagc tagaaaatta aggtcatatg gtaatgagtg ctttataatt ttcgttacag
    19621 ttttaaatga atttgtattt gatgcttttg aagtagatgc tataaattat ctgataaaac
    19681 ctctatctaa tgagaaacta tataaaactt tggataaaat agttacaaaa cttgatgata
    19741 atgaaagtaa ttatatcaca atacaaaaag gttattcata ttggaaatta aaacttgctg
    19801 atattttata ttgtgaagtt ataaaaagaa agatttatgt tcatttaaag aaagagactg
    19861 tagattatta tgagactatt gaaaatttag agagaagttt accaacaaat ttttttaggt
    19921 gtcatcgtag ttatataatc aatttacaat atgtatatgg atatgaaaat ggtaatgcag
    19981 ttatggaaag tggagataga atacctgttt ctagactacg tcaacaaata ttttcaaaag
    20041 ttatgcttga gttcatgaaa aataggagga tataagatga ttaatttatt ttcttccgat
    20101 ttatttttaa agtacagttt tttcttctct tttattgtat atagaatttt aatggtaata
    20161 gtgcaatact attttctaat caattttaca aattttaatt caaaattttg gtgttttatt
    20221 ttatattcga taatttcaat ttttttaatc attataaata catattttaa tttatataat
    20281 ttattttctt cacagattct attttttctt atgacttttt tatttgtaaa gtacataata
    20341 aaacaatcta ctgttttatc tatagtatca actttgttag tatatttaat tgaacaatta
    20401 gtatatggat ttacagctcc attaaattac ttgatattta ctagtgatat aaatacagat
    20461 atttataaat ttgatataaa tatgggacta tcttctttaa ttactattat aatagctgga
    20521 tttatatatt ggtatagttc taaaaagttt aatattaagg tcatgaactt tgataaatat
    20581 atagcaattt tgatgattcc attgttgctt ataatattat ttatgcaatc atttgaatat
    20641 agttcaaata taaatataga tacaagtctt gggattgtaa agctgctatt aaatacttct
    20701 ataactgaag aaattcaagc ttttcttttt tctataattg gaactatatg tgtatttttt
    20761 tctctattta cctttaagaa actaattcaa gctctcgaag atgacaagga gagggcaatt
    20821 atgaatcaac agattcatgc tcaaaaaaac tatattgaag aagcgaaatc acgattatca
    20881 caaactatat cctttagaca tgattttaat aatcatttag ctattgtcaa tggattgttg
    20941 aaaaaagacc aaattttaaa agcacaagat tatttaaata agcttgaaaa agtaacaagt
    21001 gacctatcat tttcttgtaa tacacagaat attgttattg atgctctttt caacaataaa
    21061 ttaagtatag caaaacaatt aggcatagag gtagattgtg aagtaattat accccaaagc
    21121 actaacatga acgattttga cctatgtata gtgtttgcta atgctattga caatgcaatt
    21181 aaagcttgta gagttgtaga taaacaaaat aggtatttaa agctttccac taagttagag
    21241 ggtggatttt ttatgataga aataaaaaat agctataatc caataaataa ttatacaagt
    21301 ggctcaggta taggtctttt aaatataaaa gaaattgcta aaaaatatca aggggctata
    21361 agtatagata agacatcaga ctattttcaa ataaatgtat tgctagttat ttcattacat
    21421 tgcgctagta tttgagaaca atttcattga aaatagtatt tgtaagctat attatgtatt
    21481 tgaaaagaga aagtttgata tatagaaatg aggaggttac taaaatgaaa gttggaaata
    21541 taaatggtca agcaccaatt acaaatggtt tagatagttg tcaaagtgag gtacaaggct
    21601 tagaacaaaa gaaaaaagca ttacaggatg aaatagagaa agtaagaagt gataaaagta
    21661 ttgatggtga aacggccaaa ttaaaaatag aagctttaaa aaaagaaatg gaagagattg
    21721 aaaataaaat tcaagagtta aaaggaaata aaggaaaaaa aacaaataaa gaagaaaata
    21781 acgtttcaga agtaaataat aatctattag agagaaaaaa agaaccttct aataatgaaa
    21841 atgtaaaaca attatatgat gtttttgaaa gaagcaatgt agacgaacaa tgggataata
    21901 cttatcgtgt tctccaagat gataaaaact taaaagtaaa atttaataga cctatatagt
    21961 ttatttataa aaatagttaa tattttaatt atcaccactt tttagaagaa tacacacttt
    22021 attttaaaga cccacatatt tatttacagt tagcacattt atatgtggtt ttataactat
    22081 tatttacaag tctatattga taacttttac atagtatatg atgtttaaat acttattcaa
    22141 atttatctat acataaacaa aaaaagagat tttaaaatct ctttttagat tgttgactta
    22201 tggctttttg atttaataaa atcaagaagc cgtttgaatt ttatatgttg gctgataccc
    22261 ttctgatatt gaaacaccat ttttgtttcc aaaatcgttt aaaattaaga acttattatg
    22321 aatgactatt tcattatgaa caataagcaa ataacatgaa tcttcaatca atgttttaac
    22381 agtagcatta aatgcttgct tatatagttc tatcgtttgt ttgaactgcc cattaaagta
    22441 aatatgagtc aataacataa ctatatctac acaaattaat ataaattttt tatactgtac
    22501 attatctaac tgttgattat ctgtattaca acatgattga atttaaaaag ccatcaacac
    22561 atagaatgtt gatggcctga aaatagattt taaaatctct tttttattat atattaatta
    22621 ttttttaaat ttatattttt tgcatcagat tttctaatag ctaagtcaaa ccctctaaat
    22681 ctaatcacta tagggtcatt aagaggagct atctttttca cttcaacttc agtgccatct
    22741 attagtccta aggctagaag tctcttcata agtttttcat ttccagctat attgtctata
    22801 attccctttt gtccaagttt taagttgtaa acagtcatta ttaacacctc ctaatgaaaa
    22861 tcattatcaa actttatatt tataatatat aacaaaaaga gcaaattgtc aatatataac
    22921 aaaaaataaa catactaagt tagtatgttt ataaaaaata gtatacatgg gatactaata
    22981 gcatctagca aaaaaccact tactaatggt acaactaaaa aggcttttgg agaatcacca
    23041 tatttttgtg tcaaagaact catatttgcc agagcgttcg gagtagctcc tattccatgt
    23101 ccaatcaaac cacttattat gatggcagaa tcaaaatctt ttccaagtac tctaaagcaa
    23161 ataaatattg aaaataggat tataaagatt acttgaaaag ttactattaa gagaatagga
    23221 ggtaataatg tagataattc aaataaatct atattcataa gtgataaagt taaaaaaatt
    23281 cccaatgaaa gttcttttaa aaaatctata agtttaaaat ttaattctag tatatttatc
    23341 ttatcattta aatttctaaa aataacagcc gaaaacatac aagttacaac agagggtaat
    23401 agtatattaa acgttatata gaagcatcta cttataattt ctcctgtatt tatacaaata
    23461 aatatgagaa gaacctgttc caaaaataca gatggtgtta ttgtttcgga gaagttatta
    23521 ttttttatcc tattcttatt tctaaaaaat ttacttccaa atctctttag atttctatta
    23581 tatctaggaa tcgacaagtc tatatttgat gatggtttta atttatattt atttattaga
    23641 aatttagcta caggacatcc caatatacca cccataataa gtcctattgt tgcagcagat
    23701 aatccaaccc caactgctcc ttctatacca agattttcta ttgtaagtcc aaatgcagca
    23761 gaataaccat gaccaccttc catagataca tttccacaca taagccctaa aagaggttca
    23821 agttttataa tctttgatat tgtaatagct aatatatttt gacaaaatgc caatactcca
    23881 cacaaaatcc aatatctaaa taatacacta ccacctttct ttataagatt tatgcttatt
    23941 tcaagtccta tagttgtaaa aaagaaggtt atgaaatttg gcattaaatt tccacttata
    24001 gatatatctg ctatattaaa atatttaaaa aataaattta gaaaacaaaa taatagacct
    24061 cctattactg gtgcaggtat acagaaatta tttaaaaagt taactttatt cttcaataaa
    24121 ttaccaatta aaaagaagaa tatagatagt gtcaaggttt gcactatatc aaaacttaag
    24181 gtaatcaaaa taagtcctcc tttgtaattc aaagttttaa ttatataact taaattctta
    24241 tgtattgaaa ttttattatc aaatgtatat taataaatgt atattaaaaa gaatcagtag
    24301 atataattag atagtcataa attaaattaa aatattttaa tgagatatga cttttttaga
    24361 attaaaaacc gtagtatatt gatataatag aaataagact taaaaatctg ttgatagtat
    24421 tatttataag tacagtagga ggataaaatg tttgaaaatt tacctaagat agatttacat
    24481 tgccacttag atggaagtgt tagagtagaa accatgcttg atatagctat taaagagaaa
    24541 atagatttac cttcaaataa tatggatgaa ataaaaaaat tagcaaaggt ttcttttaat
    24601 tgtacttcac tagatgaata tttagaaaaa tttgatttac ccttaaaagt aatgcaatct
    24661 aaggaaaatt taaaaagaat tacttttgaa cttttggaag atgcaagtag agaaaatgta
    24721 aaatatattg aaattagatt tgcaccatta cttcacaccc aaaaaggtat gagtgttaaa
    24781 aatataattg agggtataat tgaaggaatt agagaagctg aaagtattta tgatataaaa
    24841 ggcaatttaa tattaggttg tatgagaaca atgacctcaa aagaggcatt attagtaatt
    24901 gaagaaggga aatcatttgt aaataaaggt gttgtagcag tagacttgtg tggtccagag
    24961 aaagagggat tttgtaagga atataaagat gtttttaaat tagcaagaga atatggatat
    25021 aaagttacaa tacatgcagg agaagctgca agtggagaaa atgtattgga tgctataaat
    25081 attttaaagg cggataggat tggtcatggg gtaaaaatta aagaccataa aaaggcgtat
    25141 aatttagtta aagataaaaa aatattgctt gaattatgtc caactagtaa tgtacaaact
    25201 aagacagtag attcttatga agcacatcca ttttatactt tttataaaga taatttacat
    25261 gtaagtataa atactgataa tagaactgta tcagatataa atttaagttc agaactaaat
    25321 gttatatttg atacttttaa actaggatta gaagactata aaataattta tagaaatgca
    25381 gtagaagcta gttttgcaga taaagaaact aaagagtatt tgaatagttt aatttaatag
    25441 ataaaaatat ttaaacttta aattaagcta ttaaattgat aaaaatacat tgtattagga
    25501 gaaaaaatgt gaaagattat gaagatgttg tttatattaa actagtcaaa aaagaacttg
    25561 aagaatggaa gaaaaatata ttaaagaaac cttctatatt tgacaaagca tcaaagggta
    25621 tacagaataa atttaatgga gtattgccag ataaatatca tgaaataatt acttcttcta
    25681 taaaagtaat gacaaaaacc gtactgtttg gatataaata tgctacaaag ccatcactta
    25741 gaaatatgtc attagaagaa agagaaaaat tagttttaga aaaagctaaa acttacaaga
    25801 ctacagcaat gattgaaggg gcaggaacag gggcaggagg aatcctctta ggtttatctg
    25861 attttccttt gcttttaggt attaaagtga aatttttata tgatgtagct tcaatatatg
    25921 gatatgatgt taaagattat agagaaagga tttatatatt aaatatatta cagttggctt
    25981 tttctagtca acagcatata aacaaaactt ttaaattgat gaataactgg gatgaatatg
    26041 taaatagtct accagaagac atcaatgatt ttgattggag aacatttcaa caagagtata
    26101 gggattattt agatttagct aagtttttac aactaatgcc tggaatagga gcatttgtag
    26161 gattttatgt gaatggaaaa ttaattgata aattatgtga aactgctgtg tctgcttata
    26221 gattgaggat tcgagagctt aagttataag agaaattata acataaaaaa ttagttatac
    26281 aatatatcta tatgtatcta attttgcatt gataaaaaca attatggata aaaggtaaag
    26341 catgatagaa tctaatgtag aggtttgtat tttaatggaa aaatttagtt tttattgctt
    26401 aagatacaaa ttgaattacg aagagaagta attgggagga atattttcat gacaaatttg
    26461 ccaaaacaat ttgatagctt caatgaagct agacaaaaag gatttataaa tgcaaaggaa
    26521 cttaaggaaa gtgggaaaaa gatggttgga gttttctgta catttactcc agttgaaatt
    26581 ccaatggcag ctggagcaac tgtagtaggt gtatgcggcg ttagtgaaga gccaatacct
    26641 gatgcagaaa gggtgcttcc aagaaatctt tgtcctttaa ttaaatcaag ctatggacat
    26701 gcaataacag atacctgtcc ttatttctat ttttctgatt tgttgatagg agaaaccact
    26761 tgtgatggta agaaaaaaat gtatgaagaa cttgcaaaag ttaagccaac atatgtaatg
    26821 catttgccaa atacttcaaa aggagaattt gcctataaac tttggaaaga tgaaatgata
    26881 agattaaaag aagaggttga gaaatcttta ggtgtaacta taactgaaga agatataaga
    26941 actgctataa aagataaaaa tgaagaaaga gaacttttaa aagagtttta tgcacttggt
    27001 aaattacaac catctgcact tacaggcctt gagttacata atgttttata tcaagcaggt
    27061 tttaaatttg accgtgcaga acttaaacat tctttaagaa aagttataga tgatatgaaa
    27121 gaaagatatg aaaaaggtga atgtcctgtt caaaaagata agccaagaat attgataaca
    27181 ggttctccta ttggtggaat aagcgaaaaa atagttaaaa ctttagaaga tgcaggggca
    27241 tcagtcgttg catatgaact ttgtggagct attagatcaa acgatttatt ggttgatgaa
    27301 gaaatagaag atgtatatga tgctttaaca caaaaatata taaatatagg ttgttcttgt
    27361 atgatgaata atgacaatcg tattgaatta ttagatagaa taatagatga atataatgta
    27421 gatgctgtaa tagatgtagt tttacaagca tgtcatacat ttaatataga aagttataga
    27481 ataagagaat ttgttacaaa agaaaaaaat aagccattta tgtctcttga aactgattat
    27541 tcaaaatcag atactgaaca attaagaact agatttgaag cgtttgtaga gatgttgtag
    27601 gaataaataa aagtaggagg aactatgttt agtataggag ttgactcagg gtcagtggca
    27661 acaaagggtg tgctatttga tggtgaaaaa attattaaga aaataattat accaactgga
    27721 tggagcccta aaagtacatc taaacaggtc tatgagttgc taagtagtga aattgacaaa
    27781 aaagatataa aaaaggtggt aggtacaggt tatggaaggg gagtaatgga ttttgctgat
    27841 aagaaagtaa cagaaataac ttgtcatact agaggtattt actttttaaa caagaacata
    27901 aggactattt tagatgtagg tggtcaagat agtaaagtta taaatttaga tagagatgga
    27961 aatgtattta actttataat gaatgataag tgtgctgcag gaactggtcg atttttagag
    28021 attacttcta accttttagg aagcgatatt gaaagtatag atacattagc taaaggatat
    28081 gaaccagtta atatatctag tatgtgtaca gtttttgctg agtctgaaat tgtaagtctt
    28141 ctagctcaaa acatttctac tggagaggta gcagcaggga tacttaaatc tattgcaaat
    28201 aaatccacct caatgttagc aagaggtgag gtaatagatg aagttgcatt tactggaggg
    28261 cttgcaaaaa gtaaagaact agtaaaaatg atagaagaaa tactgggtaa aaaaattttt
    28321 attgcagaag atactcaaat aataggagct ttaggagctg ctgtaatagg atttagataa
    28381 aaacatataa aaggaaaatg agaagctgaa tttattataa ataaattcag ttttttattt
    28441 tattttattt atttataaag gaaaactttg gtataattga tttaaatgat tgtatatttg
    28501 taaatacttg atatttagtc atatataaca ttataatttt aaaaattagc tcaaaataag
    28561 ggggagaaag cgtgaaaaaa tttacttcaa aaaaagtaac aaggtttgtt gcgctgtttt
    28621 taatatctat tttagtaatg tcaacaattc ctgtatcagc tgacaatagc aatacattga
    28681 atcagataaa aaaagcagaa tacagtgaga cgtataaaca atatctaaat gatgctaaaa
    28741 atggacgtac agaaaaatat aatgggatta taccaaatcc atatcagttg gaaggtactc
    28801 aaatccaaag taaaggaaga acagctccaa catcttatga ccctagaaaa ttaggtctta
    28861 tgacatcaat aaaaaatcaa gaggatttag gaatctgttg ggattttgct gcaatggcaa
    28921 ccttagaaag tttcttaaag ttaaataatt atggagacta tgatttatca gaagaacact
    28981 taagatggtg ggcttcagat ggtgaatatg gatggtctgt aaatgatatg aatggtgctt
    29041 taaattatga agctatggga tatttaacat cttggtcagg tcctaaacta gaaaaagata
    29101 ttccatacaa tggtagagta tctaaagcac aaggagcaaa aaaaccaact aatatgaata
    29161 cagcacctac tgtttttaat gtgacagatg ctgtgtgtgt aagtaatgac ataaattcta
    29221 caaaaaatgc tatattacag tatggagcag taacatctgg ttattatgaa gatattaaat
    29281 atcaaagtga tgaccaaaat tcatattatt gtccaacaaa aagttttaat acaaatcatg
    29341 ctatttcaat tgtaggttgg gatgataact attctaaaga taattttaat gcaaatatta
    29401 gaccatcaaa aaatggtgct tggttaataa aaaatagctg gggagattat aattctgagg
    29461 gtggatattt ctggatatct tatgaagaca agacacttat gtctgatata gataattttt
    29521 caatcaaagg tggtaaaaaa ccaaatgatg atgagaagat gtatcaacat gactatgcaa
    29581 gtattgtacc tttaatttca aaaaagataa cagctgcaaa tgtatttgat ttcaatagag
    29641 gcgatgaaac tctaaaatct gtaatgttct taactgaaag tataggagct aagtatgaag
    29701 tatattatgc tccagtagta aatggtatac ctcaagaaaa caatatgaaa aaattaaaag
    29761 aaggtactgc ccaatattca ggatacataa cagttccaat tgattctttt gatataccag
    29821 aaggaaaggg tgcaattgta gtcagtatag aggggaaaaa tggagagtca actataggtt
    29881 ctgagtcaaa tgtaccagga tatgatacat ttaaagcaaa agctaattta ggtgaaagtt
    29941 atatcataga taatactgga aaattctttg atataaatag agatagtaac ttctatccgt
    30001 gtaattttac tataaaagct gttacagaaa agtcttctgg tgagagtatc ccaaatgagt
    30061 ctttaattgg tagtgataga tatgaaactg ctatcaaagt tagtcaaaat ggtttcaatt
    30121 cttcagaaaa tgtagtttta gtaaatggca gttcaatagt agatgcattg gcagcaactc
    30181 cttttacatc tgctataaat tcaccaatat tattgacaca aaaagaagct ttaaattcaa
    30241 aaactaaagc tgagatacag agattaggtg ctaagaaagt atacctaata ggtggagaaa
    30301 attctataag taaagaaatt gaacaacaat taaaaagctt aaatatatcg atagagagaa
    30361 tatcaggaag tgatagatac aagacaagtt tattattagc tcaaaagtta aatggtataa
    30421 aaaatgtatc tcaaatagca gttgtaaatg gagtgaaagg attagctgat gcaattagtg
    30481 taggcgctgc agcagcagaa aataacatac caatcatact tgcaaatgaa aagagcgaat
    30541 tgcaaggagc tgatgaattt ctaaattcat taaatataga gaaatcttat attattggag
    30601 gaacagcatc cctatcaaac aatctagaaa gtaagttaaa aaatccaact agattatctg
    30661 gtagtagtag agacgaaact aattcaaaga taatagataa tttttataaa aaagatactt
    30721 taaaaaatgc atttgtagtt aaaaatggaa tcaagaatca aaatgattta atagatggat
    30781 tggcagttgg tcctttagga gcaaaaactg ggtcaccagt aattttagtt ggtgataaat
    30841 tagctgatag ccaaaaagaa gttcttaaaa ataagacttt agaaaaagta actcaagttg
    30901 gtggaggagc aaataaaaat gcatttaaag agttagtaaa attaaaaagt gctaaataga
    30961 gtacttaagt attaacatat attagtgtgt attagtaact tatcttaata aaagctgaaa
    31021 ataattactt caaattattg ataaataaga taatcaatga ttgtaaagtt ttgttttcag
    31081 ctttaaattt atgtagtata agtttaaaaa cataaaaaag ggttacttta tatagaaata
    31141 tagaaaatct tacattttat atataaataa cccatattta attaactttt tatagtgttc
    31201 taatagatat tattgaactt aatggtaaat ataatgtgta agtttcattt aaagttggat
    31261 tagattcatc tatatatctt gagcaaagtg taattgataa gctatctgct tctataattt
    31321 caccatatag aggacaactg ctaaagtaag aatgtacttc aacatattta tttaagtttt
    31381 cttgaacata atcgtaaaaa cggttttttg tagtagtagc cataaaatca actcctttat
    31441 tttttattaa agttgaaact tttaaaaaga taaatatgat tacttctatt agaaactaag
    31501 ttaaaaaata agcaaataga tatgaaaatt agaataatat attatttata tcttaattat
    31561 aacactagat aataaaaaat gctatataaa attcagaatt ttcgaaagtt tttaagctta
    31621 tttttaaata tactaataat actattgaca ttagatagaa aaagtattat attgactata
    31681 taaattattt tagtcaaact ggagggaaaa gatgcctaaa tcgtattctg ataaagaaag
    31741 agagtatata ataaaaagac ttaaagaaga agcaagattg tgtatggagc aatatggtat
    31801 tagaaaaact actgttgatg agcttgtaaa acgggtaaaa ataccaaaag gaacttttta
    31861 tctatttttt caatcaaaag aattgctctt ttttgaggta ttaagagata tacatgactc
    31921 tatacaaaaa gaaatcttat atgagataaa tagagtagat gaaagtatta catgtgaaca
    31981 attgacagat atatttatga aattctatag gatggttgat agtacttcaa ttttaaatct
    32041 tatgataaat ggagaatttg agatattggt gagaaagtta ccagattcta taattgaaga
    32101 acactttaga catgatgact ttgaaattgg agagattata tcttgcatac caaatgcaaa
    32161 aaataaagac atagaaagct ttagtggagc atttagagca gtttttctta ccatgctata
    32221 taagcgcgag gttggaagta attgttttga agatgcttta agattaatga taaacggatt
    32281 agttatacaa cttatggaat agaatttaaa ttttgtttgc taataacttt tattaaaaat
    32341 tattaacaaa caaaaaaatt atattttatt gaccatatag tcaaaatagt caaaaatttg
    32401 aaagggtgat attgaatgat taaagttgat gatttatcat ttagttatac agagagagat
    32461 tttttacaaa atattaactt tggagtagga aaaggggaaa tactaggatt tttaggacca
    32521 tcaggtgcag gaaaatctac attacaaaag attctaattg gaatgataac taattatgga
    32581 ggaagtgtca ttgtaaatgg agtagagagt aaaagacact caaataaatt ttatgaaaat
    32641 ataggagttg attttgaatt tcctagttta tatgaaaagt tgactgcaat agaaaattta
    32701 aaatattttg ggtctctata ttctaaaaaa ctactatcta ttgatgagtt attaaaatca
    32761 gtaggactag aaaatgaagc aaataaaagg gtatctgagt attcaaaagg tatgaaatct
    32821 cgattaaatt ttataaaagc tttattacat aatccagata ttttattttt agatgaacca
    32881 acaagtggtc ttgacccatc aaatagcaag gttatgaaag atataatttt atcagaaaaa
    32941 tctaaaggta aaactattat tttaacaact cataacatgt tagatgcaac agagctttgt
    33001 gacagagtag cgttcattgt aaatggtaaa atatctgctt tagatacacc acataatctt
    33061 attatgtcaa aaggagctat taaagttaga tatacttact ttgataatgg agaaaaaact
    33121 tctgaatgtt ttctgaacaa tactgctaat gataaaaatt tgaatatgct tattgagaaa
    33181 aataaacttt tatcaataca tagtagtgaa ccaacattaa atgatatttt tattgagata
    33241 acagggagga atttacaatg aggctaaaaa atcttatttt aggagatata aagtttcaat
    33301 ttaagtatgg tttttatttt ttatatcttt ttttaagtat tatatatatt tgtattatca
    33361 atgtatttcc aacttttatg agagaaaaaa tagctataat tatgatttat tctgacccag
    33421 cagcaatggg attgtttttt atgggagcta ttgtacttct tgagaaaagt caaagggttt
    33481 taaattcctt agctgtatct ccagtaaaag tatctgagta tattttatca aaggtaattt
    33541 cattaggagt aatatctagt attgtagcta tgtttattgc tattacatta aatttagata
    33601 atattataat atcaactata ggtacctttt tttcttctat tatattttct ttgcttggat
    33661 tgatacttgc aagtaaagca tctagtttga atcagtttat tgttttaagt attcctattg
    33721 agattatatg ctttattcca ccaatactca atgtacttct tgacacaaaa tcatatgcaa
    33781 atttgtaccc ttttaatata tgtatttctc ttattagtgg agataaaaat tttataatga
    33841 ttaatatttt aattttaata tcaattataa taataattta ttttataact tattatttta
    33901 tttgtagcag ttggaaaaag gtaggtggag taaaactatg ataaagatta taaatgcgtt
    33961 taaacaagag tttgaacaaa ttaagagaga tgctatgctt tttatagtat gtgtttctcc
    34021 aatattatgt ggagtattta taaaatttgg aataccatta atacagaata tatcacttaa
    34081 caagttttat tatcaattaa atttagagcc gtatttcttg atgtttgatt tacttttagc
    34141 ttttattaca ccttttatgt tcttttttgc ttctacaatg gtcatattgg gagaaattga
    34201 tgacagcatt tcaagatatt taatagtaac tccgcttggt aaaactggtt atcttatttc
    34261 acgatttgga ataccaggta tcttagcatt tattattact atgattctac ttatcttttt
    34321 ttctctaact aaaatttctt tcttgttaaa cttatcaatt tctttattag ttttattaca
    34381 aggaattatt atatcaatat tagtgatttc tttatcatca aataaactag aaggaatggt
    34441 aattacgaaa ttctcaggtg tttttatgat gggtatttta gcaccatttt ttattttaaa
    34501 taaagcacaa tatattctgt tcttcttacc aactttttgg atatcgaagg catttaagga
    34561 agataattat gtctatatgt ttatttcaat tgttatatca ttaatatgga ttttattatt
    34621 atttaaaaag tttagtaaaa aaatagttaa ataagaaaat ttaatatata aatgtaaggt
    34681 tgacttgaca gtaatgttga aaaatgttac atttaagatg taaagttaac cttacatgaa
    34741 tggagcaaat atgaaaaata aaataaagat attaagagaa aaattaggat tgacacaaga
    34801 acaactaggg agattagttg gaacatctag acaggctata aatgctatag aaactggtaa
    34861 aaatgaacct tcaatatggt tggcatatga tatttcaaga atttttaatg aaccaattga
    34921 aagtgtattt ttatttgaag aaagtgaaag aaaatcaaga gcacagataa gtaggggaga
    34981 atattatggc tataagaaag ataagaacat ttgatgatga aattttaaga aaaaagagta
    35041 agtatgtaga aaacgtagat aataagataa gagaaatatt aaatgacatg gcagagacca
    35101 tgtacaatac tccaaatgga ggaggattag ctgcatgcca agtaggagta ttaaagcgat
    35161 tagttgttat agatttagga gaaggactta ttaagcttgt taatcctgaa attattaaac
    35221 aagaagggga acaaattgtt gttgaaggat gtttaagttt tcctgaagta tggggtaaat
    35281 taaaaagacc aaaaaaagtt acagtgcaag ctctaaacga atacggtgag aaaatagaaa
    35341 ttaaaggttc aggttttatg gcaaaatgtc tttgtcatga gatagaccat ttaaatggta
    35401 tagtttttac agataaaatt attgaacatg taaaattgta agatagattg gttattgaat
    35461 ttataaaata ttttatagta aggagttaaa ttatgagatt atggcataag gatttaattg
    35521 atgtattacc taaaaatcaa ttagtaagtc aatggagaga gctacttgct ataaaaggtt
    35581 ctatagataa aaaaggtact ccaaaccatc tattagtgaa taaagtattg aattatagta
    35641 tagatgaatt taaattttat acaaaaattg tacatgatga aatgttaaaa agaaattata
    35701 aacctaatga acttaaatat acaagtatat taaaatggaa aaatagaaat tttgcaaatg
    35761 atatatctaa tgaacatagt ttaaatttag aaaatttata tgatgattgg cataataaaa
    35821 tgtatttaaa gcaatgctta tataatttag aagaaaaagc aacatgtgga ggaataccaa
    35881 taaatgaatg gaatatatta ctttgtaaat atagtaaaga ttatgaattg tggtctggaa
    35941 atatcatgtt ttaattacta tagattaaca ttatagtatt attgtatata aagaggtgat
    36001 tatttgtata ataaaataaa atatattcca ataaattgtg aaatagcttg taataaatta
    36061 aaaaggagta tgccatataa atgggattta aatatttatc gtggatgtga gcatggatgt
    36121 aaatattgtt atgctattta ctctcataag tacatcaact ccaataatta ttttgaagag
    36181 atttatgtaa aaacaaatat agtagaaaaa cttgagagac aacttaaaag cagtaaatgg
    36241 aagaaagaag taatcaatat aggaggagtt acagatagtt atcaaccaat agaagctgac
    36301 tataaaatta tgccagaaat attaaacctg ctaataaaat acaagacacc agctattatt
    36361 tctacaaagt cagatttaat tttaagagat tacgatttaa ttgataagct atctaggatt
    36421 acatatatca atgtagcatc tacaattacc acagtaaatg aagatacaca aaggcttatt
    36481 gagccaaatg gtgtggattc aatgagacga tttaaaatgt taaaagaatt tagaaaaact
    36541 aacgcatcag ttggtctaca tattatgcca attataccat atattacaga tgattttgat
    36601 aatataaatt cgctatttag acatgcaaaa gaatgtaatg tacattatgt tttacctggg
    36661 actttatact taaggggtat aactagaggt gttttttttg actttgttaa aaaagagttt
    36721 cctgaattat ttgataaatt atcaatacta tatagtgaag gttcacttaa tagggagtat
    36781 aaaaatcaat tgtataaaat ggttaatgaa cttagatata aatattctct atcaagcagt
    36841 tactctaaaa taatgaagga aaagttaaaa aactcaggag atgtacagat atcttttttt
    36901 gattagtttt atataaaaaa atttttttta ctatgatgat taaaatttat taaaattaaa
    36961 atttattgac aaatataaaa tttaaatata taattaattc ataaaataaa aatatgaaag
    37021 gatgatgttg ttcggtgata tttgatatca taaaatcgtt gatataagta ttgctggaaa
    37081 agagtgaata aaatcaaatt taataagcaa acaaagtaaa gctatagttt tattgttgtg
    37141 cttaaaaata tttggttcta ttttactctt atttaaactc atctttttga agttaaacca
    37201 cagagtaaac tctgtggttt ttttatttta aaaataaatt ttaaggagtg gtatgttttg
    37261 ttaaacaaat tatgtatgta tttagaaaga ccagagcttt ataaacaaag tgaaattaat
    37321 ttttgggatg atgagtatat ttcagagcaa ttattaaaag cacacttaga cactaatttt
    37381 gaaggagcaa gtagaaattt taactttatc gaggattctg taaattggat agttacagtt
    37441 gcaaatccag ttaattatcc taaattactt gatttaggtt gtggtccagg tttatatgca
    37501 gagaaatttg cacaaaaagg ttataaggta actggaattg atttctcaaa acgttctatt
    37561 aactatgcac aaaatagaaa taaggagact aatttaaata taaattactt gtttcaaagt
    37621 tatttaaata tgaactataa tgaggaattt gatttggcta ccttaattta ttgtgattat
    37681 ggagctttgt caactgaaaa tagaaggtta ttaatggaaa aaatttatga tagtttaaaa
    37741 cctggaggaa aattaatatt agatgtattt acaataaata aatacaataa ttttgaggaa
    37801 attaaatatt gggaaataaa tgaagatgga ggattttgga gtaatgaaaa atatatgtgt
    37861 ctacaagata attgtaaata caatgattat aatacattag aacaaactct tgtaataacc
    37921 gaaaaagatg aaaatgtatt ttatgtttgg aatcactatt tttcaaaaga aagtttctta
    37981 ttagaagttg aaaatattgg atttaagtct gttgagtttt ttagtaatgt taagggtgaa
    38041 gcatattctg acgatagtat gactatggga ttagttttac aaaaataata atttttttaa
    38101 aatgaataaa taaaaacttg aaacctttaa caataaaata acataagaaa agcttacata
    38161 ttgaaaaatg gaaactttta tgttattttt agtacattac aaatttgttt atataaaaat
    38221 caatacttga attattattt ttatgtgaaa tttatctata gtatattttt ataaacaagt
    38281 aatgttatta aaattaagta tatagttaaa agtgtatagc aaaaaggctg ttatattaat
    38341 gttagaggtt cagggaaaat atataaagca aagatattta cagataatgt agattgcaca
    38401 gctataagtt agataataga attatgtaat caagaattta caacaaagta taagataagg
    38461 ataatgctaa attgccatac aggtgctaga tgtgtaaatg gatttaatta atactattga
    38521 taaagtgcaa caagcacata aaccaattga tgagattatt gaaactataa gagatatagt
    38581 tgacataatt aaaatattgg aacctgttta taattttaaa tcaaatcaat aatgattaag
    38641 attaaaataa tattaaggca tcaatgttat aagagtacaa tataaggagt actatatagt
    38701 actccttact tatacatatt aggcaatagg gaattttttt atagttaaat tttcaaatat
    38761 tttttaggga actttataat tgagaattaa gggtatagta tgtgatgaac ttactaaatt
    38821 tataattagg ttatataatg gaataatcat caatagtttt actttaggga aaatatatgc
    38881 ttaaggaagt atagcttact aatattatta aaatgtatat ttaaaatgat aaaaaaagaa
    38941 ttagacaagt atttagtaaa ttgactatgc aaaattctat caagcaggaa cactatgact
    39001 ttagtcatgg gaggttcagt aggtaacttg ttgaattaag tttatattat ttaaataaac
    39061 tacttaattt agataatata aaaagtcaga attttaagaa tagagctata taatcttagc
    39121 tattaggaga agtactatga taggaatata ttttagtgga acaggaaatt caagatactg
    39181 tgtagaaaaa tttttacaag aatatgatat aacagcaaat tcttattcta ttgaagataa
    39241 tgaactttta cagcacatca ataatcatga gaatattatt tttagttatc ctgtgcaata
    39301 tagtaatgtg ccaaaaatat tgaaagattt tataataaat cattcaactt tgtggaatgg
    39361 taaacgtgtt tttattattg caactatggc tctatttagt ggagatggct ctggtgtgtt
    39421 agctagaata ttaaaaaaat acggtgctat aactgttggt ggattacatg tgaagatgcc
    39481 agatagtata ggagatgaaa aggtattaaa acattcttta gagtataata gaaagcttgt
    39541 aataaaagca gaaaagaaag taactaaagc tgctaaaaaa ctgaaacatg ggtcaccacc
    39601 aaaagaaggg ttgggttttt tgagtcatat tgtagggtta tttggacaac gattgtattt
    39661 tatcaataaa acaaatatgt atacagataa actaaaaatt aatactgagg aatgtattgt
    39721 atgtggtaaa tgcgttaatt tatgtccaat gaagaatcta gtaattaaaa attgtatgat
    39781 tgttgcaaat gggcaatgta caatgtgtta tcgttgtgta aataattgtg ccaaaaaagc
    39841 aattacattg ttaggtaaaa aagttgttga acagagtaat attaaaaaat atatgtaaga
    39901 aatttaaagt tttattgaat gtaaattgat gtatatttta tgttagaaag agaggttaaa
    39961 taatggaata ctacatagtt gattcatttg caactaaact atttaaagga aatcctgctg
    40021 gagtttgtgt tcttgacaga agaatacctt tagagttaat gcagaaaata gcagaagaaa
    40081 acaatttacc agaaacagca tttgtagtta aatgtaaagg aaattatgaa ttacgatggt
    40141 ttactcctaa agcagaaatt gatttatgtg gtcatgcaac tttagcagca gcttatgtaa
    40201 tatcaaattt tattgatgtt aatgtaaaaa ggatagattt ctttactcaa agtggaaagc
    40261 ttgaagttac acgaaatgga aatctatatg aaatgatatt tccagaaata atgcctattg
    40321 aaatagagtt atcaccacaa caagctaatt tgataggctg tgtaccaagt gctgtttatt
    40381 cttcaagaga tttaatctta ttgctaaaca gtgaacaaga agttataaat tataaaccta
    40441 attacgcaca attgcgtaaa ttgacagact ggttaggtat aataatcaca gcacaaggaa
    40501 gtaatacaga ttttgtatca agatattttt gtccagaatt agattcagaa gaccctgtta
    40561 ctggttcatc acattgtaat cttattccat actggtctga aaagttggga aaacacaaaa
    40621 tggtagctgc tcaactttct aatcgtggtg gtataattca gtgtgaagtg ttgaaggata
    40681 atactgtaaa aatttctggt gaagcagtct tgtttatgca gggaacaata aagattgata
    40741 tttaaataag tataataact aatttattat gaaaaataat taattttctt agtgttaaca
    40801 ataataaagt agagttaaaa agcttgattt cacttaaatc aatatgatag atgttgatac
    40861 taagattgac aagcatgatt aaaaaagata atatttaatc tataaagata aaaacacagt
    40921 ctggttggta gtccagacgc agcaatagct gtcagtaacc tgcctccttg gttgtccgtt
    40981 ctttagaaag taatttaagg aggaactagc aatggacaaa attagtggaa aattaacagt
    41041 tttatttgaa gaaccatttt gggtaggtat atttgaaaga caagatggta aaaaatatga
    41101 agcatgtagg gtagtttttg gagcagaacc taaagaagta gaagtgtatg aatttatact
    41161 tgaaagattt ttctcattag attttggtag cattaaatta gaaaaaaatg tgaccaaaga
    41221 caacattgga tacaaaagaa tgcagagaaa agtaaaaaag gaacaagaaa aagaaactat
    41281 agggactaag gctcaaaatg ctcttaagct tcaatatgaa gaaagaaaac aggatagaaa
    41341 aaacctagct aaaaatagaa aagaagaaga aaaagaaaga ctatttaatt tgaaacaaga
    41401 aaagagaaaa gctaaacata agggtcatta attttaatta aatatctttt taataattgc
    41461 tagaaaaagg tgtataaaat ttttaatttt atacacctta atttattgaa aagaataagg
    41521 ttttatattt caattacttt aaaatataaa aatatactaa taaaaattgt aaaaatagta
    41581 taaatggtat accaaatgta aatttaatgt gtttagtttt atgtctaaaa gaatacattc
    41641 caattataga acctatacta cctccaatta ctgctataga cataagagta gcttctttta
    41701 ttctccattc gtttctaatt gctcttttct tatctatgta cattgaaaag aaagcaatag
    41761 aattaatcac tataaaatat attagaataa aatttttcac agtcttgcct ccataaaaat
    41821 atattataca ttataaaaca agatatttgt tttggaaagt taaatttgta attagtacat
    41881 ataaaatatt tgataagaac aaatataata ttaattggaa aatttaaaaa aattaaacaa
    41941 ttagcaaata gggggtatat ttataagaca caaagtgata aaatctattt agaaaataat
    42001 gtaaaataac aggaggataa agcaaaatga aatttatagt agaaaaagaa gtttttgata
    42061 aattagaaaa tgtatgcttt ggtgtagtag ttgcaaaagg aatagataat actaaagaaa
    42121 tagaaagaat aagcaacctt ttagatataa gtatagatag agtagaagat tacttcaaag
    42181 ataaaaaagt aaaggaatca gaagaaatta taccatatag agaggcattt agaagtctag
    42241 gcatgaaccc aaataaattt atgagttcga tagaagcaat gacaacaagg gttgctaaga
    42301 ataagaaact tcctcatata aatcctattg tagatttagg aaactctatt tctttaaaat
    42361 atttacttcc aatgggtgct catgatatgg attttagaaa tgatgatgta tatgttagat
    42421 tttctaaaaa aggggataaa tttgtaccat ttggagaaac tgatgttgaa ttaatggaag
    42481 aaggagaatt aatttattca gttggagaca tggtaaaaac tagaagatgg atatggagac
    42541 aaggagagga aggaaaaatt actaatagct caaaaaatat attttttcct atagatggtt
    42601 ttactgatgc taatttagat aaggtaatga gtgcaagaga agaattagca aaattgttaa
    42661 aggaaatatt taattgtgaa attaaagtag gttttgtaga taaagataat cctgaaatgg
    42721 aaatataata agttgttgaa tacttgttaa gatattatat ttacttaatg atgttaaaaa
    42781 ggtattaaaa aagaaaggtc gtagtaaatt tgaaaaaaac tataataaag aatgtaaatc
    42841 caatgattga aaataagttt ataggacttt ttgaaataga gtataaaaac aaattagatg
    42901 aagataaggt ttggatggta gcatcaagaa aaagtagtga gcaattaaaa aatatttatt
    42961 tgaagaatga agaagatagt gtagatgcag ttgtaatagt tgggcttcat aaaagtagta
    43021 aaaaattaat tttaataaga caatttagag tacctattaa tggatatata tacgagttac
    43081 cagcaggtct tgttgatgaa ggagagtcta tagatatttc cgttgaaaga gaacttagag
    43141 aagaaacagg acttactctt ttagaaatta aaaaaaataa aagtattgat aaagtttatt
    43201 tatctcctgg aatgagtgat gaatctgttg cctttgttta ttgtatatgt gatggtgata
    43261 taacagatga atttttagaa ccagatgaag agattgaagc gcttttaatt tcgcaagaag
    43321 atgcaaaaga gattttacaa agtaatcata agatagacac aaaagcattt ttgattttac
    43381 aaatgtttgt gagtttagga gctaaattat ttgaatagat atatagaaaa gaggttatat
    43441 agaagagtaa aataataaag tttattttac ttgtataaat tataatctct ttttttatac
    43501 aataaagtat aattttgtgt atttttaaat aagtgacttg actcttaaat tttcataata
    43561 tgttataaac caatttaaga tactacaata attaaaattc caaaattatt taacatattt
    43621 gcaattaaaa tgagaaaagg taaaggttga atgattgaaa aatttgatgt aaaatagtag
    43681 cttagtttaa tattatagtt tgtagggtta aaaattagaa attgtaaggg agataaattc
    43741 tatgaaaaaa ttagttatta ttttaattag tatcattagt atggttagct tgacagcttg
    43801 taaaaaaagc aatgaaagta aatataataa tttaaacaac aaagaatccg gacaaacaac
    43861 cattgaaatg gacttagata aaaattatga tacttcagac ccatttgtta atgcacgttt
    43921 gttttgtgtg tctaatgata tagatgtttt agacacagag atttctttta aaatggatgg
    43981 agatagtggt attgtggaaa taaaggacaa taaaacatat gaaactttat ggagtaatac
    44041 atggcatgga agcgttgata atgatacatt cacaatttct ctttctaata tacaaaaaga
    44101 gaaagagtat gctatatgct ttactggaat taaaataaat catgcagttg taaaggttac
    44161 ttttgaaagc aatttagtta aggagaaaga aaaaccctca aaataaattc cagattgtct
    44221 ataacttgat attcagtaca taaaagcagt tgatatttat gattgactgc tttttcttat
    44281 caaaaaggag agtttgtgca tttttacttg aaatagttta tgcacatcaa aagttggtga
    44341 gcatgatatt ggaaatggta agcatgaaat cttgataaga caaggaaata aatttaagtt
    44401 ttcaattaag tatatttcca taatttagtt aacaataagc taattattat
//
LOCUS       DAMJVK010000025        44005 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.025, whole genome shotgun sequence.
ACCESSION   DAMJVK010000025 DAMJVK010000000
VERSION     DAMJVK010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 44005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44005
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.025"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(112..264)
                     /locus_tag="PC414_001375"
     CDS             complement(112..264)
                     /locus_tag="PC414_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463777.1"
                     /translation="MDNFLFNVLASLTASVVVYLISELFKKAKSHSRAKSDLQVEFKF
                     IFKFKK"
     gene            complement(446..1603)
                     /locus_tag="PC414_001376"
     CDS             complement(446..1603)
                     /locus_tag="PC414_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDF2463778.1"
                     /translation="MNQVKDQKDNILMKSNKFIMARYDLQTIENRLFVRLLYEFQKNK
                     NLEITITIEEVRELMKNPNYTTPQAISKIFTKLRTGTIYIKDDDSWGECSFIYHWIYN
                     EKDKTYTVKTSAELLDLIENYIQVGYTPINLQIFFSLRNSYAQRFYDLLRLWTRTKNK
                     ITYTLDVLKELLLLEDKYSKYNDLKRRVIVPAIQELNSTEYFDIDFKEIKKSRKVVAI
                     EFSVKDLDKRKYFDKNVVETVIYEEQEESKETETTIEFYIPDETAFTKGTIRLFKIDF
                     KEFDFKKDIYIQAFDKSLAIALERDNADIINTKNYDFFKSTLSNKISEVIIQEKDDLK
                     FKEELDKYWDDEDVKKEEREIDYSKDPVKLETVRRNLFDQGLVNEEGYYYK"
     gene            complement(2486..2869)
                     /locus_tag="PC414_001377"
     CDS             complement(2486..2869)
                     /locus_tag="PC414_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2463779.1"
                     /translation="MKIKKIPESELLVMMYIWDSEDEEVASTEILKTLGEKYEWKKST
                     MLTFLRRLVGRGFLEVVKKDRFTYYKALIEKEEYLKVETKSFFSFFHRNSFESFISAL
                     HDDEEISANKLKSLEKFIEDWEEDK"
     gene            3454..3609
                     /locus_tag="PC414_001378"
     CDS             3454..3609
                     /locus_tag="PC414_001378"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463780.1"
                     /translation="MGELKLESKMNCTKNKKKVAISIHQMKKISKKDYVFLTDCIETF
                     YMKIKEV"
     gene            complement(3635..4003)
                     /locus_tag="PC414_001379"
     CDS             complement(3635..4003)
                     /locus_tag="PC414_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2463781.1"
                     /translation="MAEVSKKLKAKEIGKRLNELRTLERLSQRAFGERIFLSQDQISL
                     LEKGKRVLTERSINDICREFEVNEEWLRSGKGEIYKDCLADLEIDDDVKEITNKLYEL
                     EQEDRDAILKMIELLQKKNK"
     gene            4175..4336
                     /locus_tag="PC414_001380"
     CDS             4175..4336
                     /locus_tag="PC414_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463782.1"
                     /translation="MICSNKEKVETALMIDYLKKNEPNEWRTIETIISSTYALSNFKK
                     ENKDIMNQK"
     gene            4368..4583
                     /locus_tag="PC414_001381"
     CDS             4368..4583
                     /locus_tag="PC414_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463783.1"
                     /translation="MKISDFNKNQIGTAVQLQDIRFGTKVDCIITKVEKNEILVMYYE
                     KETEEIAYKTLTKEDLILDDYKLKLLS"
     gene            4612..5343
                     /locus_tag="PC414_001382"
     CDS             4612..5343
                     /locus_tag="PC414_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2463784.1"
                     /translation="MDLLKDARKKEWFWLENDLVDREDLGIYEKMIYIVLARYSDNES
                     CCFPSYKTIALKCGCSKRQAKSVVKILENKGLIKKENRIKSNSNEKESNIYFVLTAKL
                     GGEYDAQQVVNMMHNPSAPHAQQVVHEVHSKKTYNKKTYIKSNTTTQKESKTDYLDLS
                     FLDLDIEKVKLTKDEYDKLISKFGKKYIHDKIVSLENYIINGKGSRYKSHYRALLTWG
                     NGDASKGIVPEVTKTKNPLSGFKEL"
     gene            5371..5556
                     /locus_tag="PC414_001383"
     CDS             5371..5556
                     /locus_tag="PC414_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463785.1"
                     /translation="MNEKEETVYLNSSNVKLFLGVRDIKEFEELIENVNERICQLQES
                     INKLAQFNIEFDIKSDI"
     gene            5582..5800
                     /locus_tag="PC414_001384"
     CDS             5582..5800
                     /locus_tag="PC414_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463786.1"
                     /translation="MKDEKLELTEKDLHCIARHLQNEVMEMVFRGNIEAPTSCEVCDY
                     LQECKDYFTHIDTFIKLSEMTGVDIFTK"
     gene            complement(5819..6031)
                     /locus_tag="PC414_001385"
     CDS             complement(5819..6031)
                     /locus_tag="PC414_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463787.1"
                     /translation="MNMCKIKTLREKCSLIDDKVEFSGEYKTFPGFTGSKLLECECNK
                     NYICKIQIMTANSNCPLFKKARQLQY"
     gene            6194..6454
                     /locus_tag="PC414_001386"
     CDS             6194..6454
                     /locus_tag="PC414_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463788.1"
                     /translation="MKEEKLELTEKELHCIARHLQNEVMEMVFRGNIEAPTSCEVCDY
                     LQECEGDFTCMYHSFTKLSKITGVKVTAFKYCFPDENPKATI"
     gene            complement(6447..6635)
                     /locus_tag="PC414_001387"
     CDS             complement(6447..6635)
                     /locus_tag="PC414_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463789.1"
                     /translation="MSQYRVISKECPVINETISLTCEYSAPPGTFGSKLMSTRCDGSH
                     ICDSSSNEECPILKKLLR"
     gene            6773..7090
                     /locus_tag="PC414_001388"
     CDS             6773..7090
                     /locus_tag="PC414_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463790.1"
                     /translation="MERKKHEFTDQDIQRLIEGLRTDPKIREMIFKSKGCETRTSTRS
                     KCEQFVEEKLAYRSYGFETPKYYAKLIADIIDILVYKNVPRDRICGVLYDVERVIPLV
                     VTF"
     gene            7112..8443
                     /locus_tag="PC414_001389"
     CDS             7112..8443
                     /locus_tag="PC414_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2463791.1"
                     /translation="MNYSLEAEQNILGSFLINDSIGYKIRELKENDFYFESHKIILSC
                     MKKIIDSNKPLDLLLLKNELEKIDRLVDIGGVSYITSLTTIVITTSNIDHYIKIIKEK
                     LLKRQISELANELINNSNSDTSIDELIIDINDLKALVTTSSSVNDNYIDASKIKREKG
                     VHKSIDTGFNKLNNMLDGFRYGTLTILTGKPASGKSTIVNQFIAQAITNGEKAFLYSG
                     ELPSFMSMDWFRKTVANDYHIKEYKSVYGGTYTDIPDYAVELISDWIEDKFFLYDEDA
                     ISDEVNLLNTIEHLYLKKGVRFFVLDNLMTIKTGNKADKYERQEQIVSNLKNLAKKYN
                     LVIVLVAHPRKNMGDMKPTMYDVSGASEIVNYADYILSTYRVVDEEEETDDTYLLILK
                     NRITGKQNISFKMNFSERRKRLYTTSEELNRDYKYDVNKQYVQVEIPEDVF"
     gene            8818..9204
                     /locus_tag="PC414_001390"
     CDS             8818..9204
                     /locus_tag="PC414_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463792.1"
                     /translation="MIALEKFAGGVLKEKFNTELQKVLDNIADPNTDFKKTRKISLEI
                     VFKANEDRDLAEVDIKSKATIVEAKATTTKVLIGKDLETGRVEASEFKNQVAGQLVMD
                     IPDSEETNETEENSAGVIDFRNASSK"
     gene            9257..9961
                     /locus_tag="PC414_001391"
     CDS             9257..9961
                     /locus_tag="PC414_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463793.1"
                     /translation="MIRNAMEYLVKLGKRETVCVDGFKYTTDALEIIKEPKANELEIT
                     TLSGLVDYIKSGIDHKADELLLIQVLTPRKVLLKSPLRKNRDRETYIECVALLPEKRF
                     DRPLDLDSFNIMLQSAFIKNNDRDTLLKIAGNIQESTVKNVGDDGVSQSVTIKTGVAS
                     VSEAIVPNRVKLIPYRTFQEIEQPESEFIFRMSSGPAAGLYEADGGAWKNQAMLNIKA
                     YLQEELKDFKNVNIIA"
     gene            10018..10200
                     /locus_tag="PC414_001392"
     CDS             10018..10200
                     /locus_tag="PC414_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463794.1"
                     /translation="MKMISHMNILERAEFTDKIANAIMSANKEIERGCAFNEAVEIVK
                     QMEYRENKYNEGDIAI"
     gene            10197..10574
                     /gene="ssb"
                     /locus_tag="PC414_001393"
     CDS             10197..10574
                     /gene="ssb"
                     /locus_tag="PC414_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDF2463795.1"
                     /translation="MNNVVLVGRLARDPELRYIPEYGTPVATFALAVDRGYTKKDGTR
                     EVDFIPIEVMGGSAEFCANYLAKGRMVSIQGQIRIEKYEKDGEKKTFTKVRTKIVNAL
                     DRKPKDEEKEISFQVLDDEDIPF"
     gene            10555..11139
                     /locus_tag="PC414_001394"
     CDS             10555..11139
                     /locus_tag="PC414_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2463796.1"
                     /translation="MKIFHFKLDGDYVRSLEERNKLVEENINFVWFIIRRYFKVFISK
                     YPYLQDDTFSEGCIGLCKAADYFDSKKGIEFTTFAAVCIKGQIFKFISRYVKKHYRAD
                     IDSFEKCIYKNTHGEEIRIEDRLAGNENVDIENVHVIRSCIKRSKIEDIQKITYLREK
                     GYTQKEIGKILRTNQMTICRKLGKLKSEINILGK"
     gene            11169..11327
                     /locus_tag="PC414_001395"
     CDS             11169..11327
                     /locus_tag="PC414_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463797.1"
                     /translation="MKTKIIGGRENQCPLCNGSVFKIETSIGIVWQCKDCGCMYQDMN
                     CKENKRRH"
     gene            11330..11656
                     /locus_tag="PC414_001396"
     CDS             11330..11656
                     /locus_tag="PC414_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463798.1"
                     /translation="MACVIKCDFCGETIAEILNIKVFRIDDEGNIMSIGKDVCDKCYD
                     RLFRSNLNKKMTNYEKIKNMSKIEMAEFLANGGSGCTSCAYDFQECLGGCLEGRKKWL
                     ESEAMK"
     gene            11656..12192
                     /locus_tag="PC414_001397"
     CDS             11656..12192
                     /locus_tag="PC414_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HDF2463799.1"
                     /translation="MKKTLIIVGVVISILIMFACLFLVTKNKAIDLEEQVQSATASVE
                     VQEKRRIDLIKNIVDCVKDYAKYENRTLKEVTDMRVSANDESIKEAKLNIQAVAEKYP
                     DLKANENYKQLMSELSITENMIAQHRENYNMQVRNYNKYIRKFPANLILNSMGYEKIE
                     SKYTEYKAKEDAPQNLFD"
     gene            12204..13010
                     /locus_tag="PC414_001398"
     CDS             12204..13010
                     /locus_tag="PC414_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463800.1"
                     /translation="MKFKTFEITKREFLVSIIIACMMIVLGFFIDDKVQSKFLEDNER
                     YYKATKINNDKDLFDYAMETSAGDSLISGRFKSVDSVSIEELKGDYWFIERTEEKYTM
                     HTRVVTTKDSKGHSHKYTETYWTWDYHDSIEKKCNKFNFMGKEFDAKVITNLSKERLA
                     LKKDIFKNNNLKIKGNYAYINNKRRYYYEVIPKEFNGTMYANLKNNTIANPADKNIKV
                     FKGESIQNVLENNNFNTGFFKVVFWIIWILITTIIIYVFYYLKNDWLEDK"
     gene            13035..13295
                     /locus_tag="PC414_001399"
     CDS             13035..13295
                     /locus_tag="PC414_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463801.1"
                     /translation="MKCKVYHAIKNIKDANELIDRFETENIFFELNDIDNENVLNGEM
                     NLTDIEDIASLQFILDGDLFIFSSCDEMIFMNKYTQKELGLQ"
     gene            13319..13804
                     /locus_tag="PC414_001400"
     CDS             13319..13804
                     /locus_tag="PC414_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463802.1"
                     /translation="MDLKLISSIPNWKVEDQLAKIDEEVTEFKNAIDTGITKDIIAEG
                     LDVCQTILTMFQVLEIENYITEGVETHNKKLNGRGWDLKEIGDDILNLIKNSMINELI
                     NMNKKLKCLDEKEFNAKIQFLDVDKLWNLCMPLVNLSTIKGVNESIDEMKMGEFLKNI
                     N"
     gene            13824..14081
                     /locus_tag="PC414_001401"
     CDS             13824..14081
                     /locus_tag="PC414_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463803.1"
                     /translation="MLIEVTENNRELKVGDIVEINGGNKLYMILSLKETIGGYVIINM
                     QNGYGSFGAYKSLSQLELDLKVRGYKLYSSDDYKLQLVPRE"
     gene            14190..14633
                     /locus_tag="PC414_001402"
     CDS             14190..14633
                     /locus_tag="PC414_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
                     /protein_id="HDF2463804.1"
                     /translation="MQSSKKDNLFSSAEGKLYDYKKIKAEIEKIDIDMQMIKNDYRGC
                     SGVEIKEKTGKTYDIKSVIEIETEEKEKKIALKEKEKRHKELIIEKIDNAMKILSEEE
                     KKIVQYKYFSSSRTSWEYVGRMIGFSASKCKQMRFDIIDKIKGLL"
     gene            14776..15321
                     /locus_tag="PC414_001403"
     CDS             14776..15321
                     /locus_tag="PC414_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463805.1"
                     /translation="MAWNLRSKSFLLSREVLKIKRDITEEISSAAYVPDNLKYYDNVM
                     KETPGMYGRECSLDFIKKKQEKLLKLKKKVKKNYDSNINKIDSYLKILEKSIIDESDH
                     VELVVFKLYLQLENVVKVTETVNDLGFRIKTNTYARERKYSTNDITSIITDPFTDVVE
                     DLKILVQERQRKNYYGNKECF"
     gene            15325..15969
                     /locus_tag="PC414_001404"
     CDS             15325..15969
                     /locus_tag="PC414_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF2463806.1"
                     /translation="MEAHSGQKVNPIKDIEDVFRLLNFLEEWNERNYLLALFGMCTGL
                     RVGDILALKVADVTDLKLDKKGNKIRVSKDWIRVIEEKRDYNREVFLSDVIKSAIENY
                     TQDKPGEEFLFKSGKRKKFNRPIQTRQAGRIIKGAAKKVGIKENVATHSLRKTFARHI
                     YDEEENKTYALELIRKILGHKTIEMTRRYIGIDKDEEVKAITKFTNKLKKRKRN"
     gene            16184..16642
                     /locus_tag="PC414_001405"
     CDS             16184..16642
                     /locus_tag="PC414_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2463807.1"
                     /translation="MDVNEVEIIDSSYEKGKELTEDQCNMVTLLITGATVTETARIIG
                     VNRKTIYNWMNKEHVRKEMDRRKQELTNQGNLMILKDLDSYINNIKELASDKSDKRVM
                     LAANQYLINRIYGTPTSTVIQAEDNSVGMGMDATEIEAAIAKIRIRTSKK"
     gene            17197..18936
                     /locus_tag="PC414_001406"
     CDS             17197..18936
                     /locus_tag="PC414_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463808.1"
                     /translation="MDNLERNIQLIFSYLAKTFIRNGASIKDAEKEANEIIKANSNNL
                     WGVNGLAYQLGKINLEFFCMYFMQDTYLPKEDNAAAPIAKVHHELWEDIQESIIGDGS
                     QQLGRIYPRGTGKSAFGDLATTVWSHCYKHKTYTLICSDIGSTAEKFVKDIKNALLEN
                     EYIKKAFGVLLNDNDRKYICNSTQLELTNKTFIEAISSSSPMRGRKYNNNRPDLIILD
                     DYQSEENVRTEDAREKKFKRFSDDVKYAAQKPVIRNGKTIKRGTTFIALGTLQHKECF
                     YSRLKNLPTWKFKCEKGVLVDNVDELFNSGLWLKFKEILFDFKNTNHLEDAKEFYWEN
                     EKEMKFHILWPSFWDCLDMALSYYENPTSFKQEVQNDVNSIGEKWFKTVRTETREEIE
                     SHTFKKTMLLCDPASAGGSKHDYSAFLVGSESENGLLYGRLAELAKINARTDFDKYID
                     HMIYLLKVYSDITHIYIEKNTFNGADANQLEFKIKNDNVLSYRDIEIINEQQKKNKDD
                     KISTLIPVLSKGQMIFAEEDKEFIQQILDFTGQKYSLHDDAPDISSEFINRINNIKVS
                     ETVNIFDRKLLGF"
     gene            18937..19140
                     /locus_tag="PC414_001407"
     CDS             18937..19140
                     /locus_tag="PC414_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463809.1"
                     /translation="MGGNLLANRSKFEEVQKTLTKKGKKFNVGIGKDEKGYFAYTHRA
                     RSKSYISKQDIPIKVLKFIESTG"
     gene            19153..20430
                     /locus_tag="PC414_001408"
     CDS             19153..20430
                     /locus_tag="PC414_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDF2463810.1"
                     /translation="MELDLDLIKDIYDDWNSNKSEYDTMYKYYKGETDAISNYKMVTK
                     RSNNKINTNFLKKFINEEVAYSLANKITYTSRSGDEKIINDLEYYTCHWSKKHDSDLL
                     RYALLFGLCYELYYVKDDEIQVKIIKPTDGCHYENENGEIVCFIREFTKGFEDDTYVD
                     VYDKEYIYHFDSNFKEVESPTVNNIFDGNVPISICKRSEELGKNTIFNDIKGLQDAYE
                     TNLSDISNEISDFRNAYLTFSGCNIKEDDLPRMKELGILQVNGDGKIEWLIKDMNDTF
                     VQNTLSSIKENMYEITSHINHNEKMQSNTSSLAVIARLISTEWICSQNNDSIADTLFN
                     RYKLLCIWLNKKYGFDYDYKDIKAKFTPKIPRDDLVVANILSQLGDKLSTETGLSQLS
                     FIDNPHAEIEKAKKEQEIVSEGEILLDESKDYN"
     gene            20408..21169
                     /locus_tag="PC414_001409"
     CDS             20408..21169
                     /locus_tag="PC414_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HDF2463811.1"
                     /translation="MNQKIITKLTEDIYKSAENRTKPFYKYQRENRDKLLLEIAKILL
                     THDIINEYLNINNKDKKNLRSKLNKIINEYSNSYDEEVKDIETILSDSSKNKYSNLII
                     LLGVILKIKDVSNINNKVINKIVNNKVDGKHWSDRLWKNKKGIEESLKKEIKSFLNGK
                     TSVNKIEKAIRDKYSTGAGQTSRLIENEVARCQSEVNEYFFKVQGIIKVMYCADLDSR
                     TCSDCSMHNGVIFYVNEDRPSLPRHCHCRCQYIPV"
     gene            21228..21554
                     /locus_tag="PC414_001410"
     CDS             21228..21554
                     /locus_tag="PC414_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463812.1"
                     /translation="MKVEILGTEYLVIEKLESEDVLLKERAGYCDHSVKEIVIEKINS
                     EEGSLKDLSVYKNEVARHEIIHAFLSESGLKSCSSWATNEEMIDFFAIQFPKIVKVMD
                     KIECLN"
     gene            21580..22182
                     /locus_tag="PC414_001411"
     CDS             21580..22182
                     /locus_tag="PC414_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HDF2463813.1"
                     /translation="MLKKEVLELLKEIEDDAEIDSLLQSTDLFKSASDKKLTIEEFRE
                     LINNDANFKAIIDNEKNKYHSEALENFKKKDMQTLISAEVLKRTGANETEEQKAIREL
                     RESLDKLKKEKQYAEKVSKYKDILVEKKIPTNLIEYLLSDDDDKTNANIEIFENSMKQ
                     YVQSKVDERIKDGSYVPPGKDSAGSLSEIRKQIKQGLNSL"
     gene            22211..23134
                     /locus_tag="PC414_001412"
     CDS             22211..23134
                     /locus_tag="PC414_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463814.1"
                     /translation="MANVLQYVTLFQQELDMQVTVGSTTGWMEKNAGQVIYNGGREVK
                     VPKVTMDGLGDYDRSKGYTQGAVTLEYETMKMEMDRGRTFMLDSMDVNESNFVINASS
                     VMGLFQKQHVIPEIDAYRYSKIFSETAKVDERVKYSYEATVTTILSELKNQLASIQDL
                     SGDIPLIISMSSIIASILEQSSQLDDIEFGDGDIKRIVKSIDNVPIIKVPSKRMKTEY
                     VFRDGKTSGQTEGGFTEGAKALDINWLITPASAPIAVSKTDKPRIFTPDQNQTADAYQ
                     IDYRKYHDLWILDEYLKLCRASVRQAKAAQE"
     gene            23148..23420
                     /locus_tag="PC414_001413"
     CDS             23148..23420
                     /locus_tag="PC414_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463815.1"
                     /translation="MFALIKNNIIKVVEDENSRDKLISKGFKIYSEEVTKENIEKKEE
                     KVKSTSNDLFNLSKPELMEIAKGKNIKGYSTKTKEELIQLLDEYNA"
     gene            23404..23784
                     /locus_tag="PC414_001414"
     CDS             23404..23784
                     /locus_tag="PC414_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HDF2463816.1"
                     /translation="MSTMLDNVKLVLGIENDRYDKLIILYINKISEMVLEYCSRQELS
                     TALEGFVEEKVISILSSRFEELKKNSGLDNLEKSLKAITRGDTKIEYNALTLQTAENS
                     ISSSSFLTDTDKKFLSPFCIVSMY"
     gene            23796..24143
                     /locus_tag="PC414_001415"
     CDS             23796..24143
                     /locus_tag="PC414_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463817.1"
                     /translation="MTEADILEMTYLDKMTIIRKLKGWNEETCSNDFSNVVIAENIPC
                     AISRKEEAIVNGDIGSIVVNRKLFCRPEVDIKIGDTINTTYNHGEEAVFKVSKANIYP
                     SHMEIPITELERI"
     gene            24143..24499
                     /locus_tag="PC414_001416"
     CDS             24143..24499
                     /locus_tag="PC414_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDF2463818.1"
                     /translation="MGIDNLGVFEALALDLGLNASNFNRKIKKFTKETGQELAENIRD
                     ETPVKTEKLKKGWEEKQPEYDTYIVKNEVDYATLVNNGHSTKNGGIVPGSFMVEKGIE
                     RTKENMTSAKANLFDW"
     gene            24508..24957
                     /locus_tag="PC414_001417"
     CDS             24508..24957
                     /locus_tag="PC414_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463819.1"
                     /translation="MLTNRDIIIAVNKRIENEFRYIDIFINEDNIQGFEEACFFVQLL
                     PVSNNIANKVLDTKKIFIDIEYYQKARKNKLNLYDIQSRLEKIFNRNIRVKDRSLIIE
                     NISPSIAKDPVGYKLTFLMTVSYFEEVYFENETFEIMESVDLEIGSD"
     gene            24958..26013
                     /locus_tag="PC414_001418"
     CDS             24958..26013
                     /locus_tag="PC414_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HDF2463820.1"
                     /translation="MGLPEINISFIQAGKTFVKRSGGIVALILKDTKNIGLVEIKEIE
                     DIPENYSAANLDYIKMAMKGNVYPPNKVLVYTLDTDESIDDALDFLETCEFNYLCMPD
                     ETEQDLPKIKTWIKKMREDNKIKVKAITASNSADYEGIINFTATDIEVEGKKYTSNEF
                     LPRIAGFIAGTPSTQSVTYAEIPEVTNIPKLTRAEANTRINNGELILIKESGAIVIAR
                     GVTSFTTITDSKGDIFKKIKLVDTLDQIHNDIKKIIVKNYIGKTQNTYDNKCLLIVAI
                     QLYLQELEKDGLIDEGSTVEINLDAQRAWLKKQNIDISNMEEQQIKEYNTDTLVFLKA
                     RIKLIDAMEDVYLDISM"
     gene            26029..26469
                     /locus_tag="PC414_001419"
     CDS             26029..26469
                     /locus_tag="PC414_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HDF2463821.1"
                     /translation="MKAKIDAKRIISGTFGECWLDNKQLTSTKGMEAKVDIDKEEIKL
                     CGQIMTTYKMIGATGKGSITLYKISTLFGEYVEKMLNEGKEFKVTIISKLQDPDSYGS
                     ERIALYGCMFDDLTLADWEAAKVGEIEVPFTFEGFEYLDKIKEK"
     gene            26484..26900
                     /locus_tag="PC414_001420"
     CDS             26484..26900
                     /locus_tag="PC414_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDF2463822.1"
                     /translation="MNTVEKLLNMDAGKLKMPSSAFELYCKKLDDTLEIECKAIDPER
                     FDEIRMNSMDVNSGDVENINIYELKVNTILESCSIFSNMELVKKFSAITPKELIKKLL
                     LSGEIDKLYEEVNKVNGIDAEGDKKRQKERAKDIKN"
     gene            26927..27076
                     /locus_tag="PC414_001421"
     CDS             26927..27076
                     /locus_tag="PC414_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463823.1"
                     /translation="MYLLFKYKGIMPSVSYHILRGEKEIVSVFMDQELDERKEEIKSG
                     VGRLF"
     gene            27080..30469
                     /locus_tag="PC414_001422"
     CDS             27080..30469
                     /locus_tag="PC414_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDF2463824.1"
                     /translation="MSSNTKVLQAIIKMRDEASSTLKRVAANTRALHDSSSQASRALN
                     NVQSNLKSVGTSALKAGGLITAGVGGAFAAATKVGTEFESAMSQVKATFGNSLKPGDF
                     EALSKAARDAGKATTKTAADSANALQYMGLAGWDVQKSMHALMPVLNLSEAASMDLGL
                     ASDLVTDSMSSMCLEVDKVSKSGKVLAGAGLSEYLDKVANASTKSNTSIQQLMEAFNV
                     AGGTFSNLKVPLSEATAIMGILANRGVKGTDAGHALNSIMVNLTTGAGQAGKAMKKLG
                     VSAFDNQGKFKGMSNVLKEVANKTKGMTDEQKNYYLSAIGGKTQLKTLQKLLDGVGNE
                     YDSLKKKIDNSKGALKTMSDVMKDNTKGGLATLKSALEETGITIYYQLKPAIASVVDN
                     LTKMTVWFNNLSEPIKRVITNVMLSVGAFGVLLLTIGAVSLIVSKAIGIFGFFVPIIL
                     RAKVAFRAFVIGMKPLSAVFLAVMGPVGWTITAVIALSVAFTIAYAKSEVFRNKVNSL
                     VKSFTEFAKPIVNFCMPALKQLGEKFKELLSAFGPLFASILNLGSVIMKVLGPVLIGV
                     IGFHIAKLVLILSTLATAFKAVVDTITNVLNGLASILNGVFDLIIGIVTGDSKKMMDG
                     VKQIFDGGTKIIKSIWKGFIDFVTAPIQAIADIADSLFKDKAKWLTDKWRDLRTFLEN
                     PIEATAKISTKTNVNIPGSKAADAAGKSTPNTGGLKQTVANLKGSFSDFKTFIKPMTD
                     SISQGVESGKTKFTELQNKILNDVKPALKVLEDSGVNFKDKFKESITSIKDKFNELKN
                     ILAGNIKTNIDNLVKAFEPLKPHLDNLKDCFDKVKKAITDFFTPAKQVGDTVNKVSQP
                     MDSVKKSTDGIKLSFQSLTQALAPVKAWFQGFMPFLSQIGQQLLPSIGIAIAGVVGGI
                     ILAFTWVLNTLTAIITTVSGIINGIMSFISGIINVIMGLITGNADQTMDGLKQIFDSG
                     VGVIKSIWNGLVDLVSGVLEPVIDLADSLFKDKVKGVKEAWNKLKDFFKHPIKGTIEI
                     FKKGPSLQIGKGADAAGKSPRKAFGINRVPRNDYLIRAHEGEKLLTKQEANQYKRNSS
                     NGSLFLDKLADTIIVREEADIDKIVRKINKKISLAKIGGVL"
     gene            30516..30992
                     /locus_tag="PC414_001423"
     CDS             30516..30992
                     /locus_tag="PC414_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HDF2463825.1"
                     /translation="MNKKILVLTISIILSLSLVGCSTNNSKNRDSKSKQNTEEVKNHK
                     IGEVVDLDGAKVSVKNIRKLETNSNDYAVEIEITNTGDNDLQSDYLENYILKSSDNTT
                     GEAINNQELNGELMFDTIRPGDTLKGEIAFELTKDATPKTLEIAYENVGKYTVFDL"
     gene            31050..31685
                     /locus_tag="PC414_001424"
     CDS             31050..31685
                     /locus_tag="PC414_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDF2463826.1"
                     /translation="MEIWLRQANNTFRFPVIPPSFEINGSATINTSNILSVGDIAVFG
                     GLGLKTIELSSFFPNQEYSFCNYNGFPKPYDCVNLIESWMKEGYILRFIITETNINFE
                     CIIADFNYREQDYSRDIYFTLSLKEYRRIQISKVNINNDEKLSSEKNVPLTKGFDTKQ
                     KTHKVVEGDTLFKIAKKYYSNGDLWEKIYKANEDEIKDPSVIKNGWVLIIP"
     gene            31926..33443
                     /locus_tag="PC414_001425"
     CDS             31926..33443
                     /locus_tag="PC414_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDF2463827.1"
                     /translation="MIIDRSKDSSNNNIDFTAKDMGFLLLQSEVSYNFKDKLVEDIAK
                     QVFVDNKFPLGNLPKTNVKYTKMFIGVTGYDTIMSVYTEASKTTKKKYMIESNLDKFN
                     VIEKGVVTLNITFEEGFNLINTNFSESIENVKNKVLVVDQYGNKISEKINDSIFKDVG
                     VIMQKVIQQQENSTIDIDGEFKGIEKTCSLKGYGDITCVTGRGVKVKDSYTKLIGLFY
                     IDTDKHTWQNGDYQIELELNFENIMDEKSAGQDEQKEDGSEYTGGTEYPAEFTAYCPR
                     KEEGGNTDCRGKKLDPSKKTCAAPMVGAYEKSYYTKDFLNKHPLMRYGDEIQLVTGVS
                     SRDGTYKVNDNGPAIIIEKDGTYHIDILFGNVEEANNFGRRKGKIIIGGYSGNMTEKA
                     KTVISEARKHLGKPYKWGGNGPSSFDCSGLMVYCFKKVNVNLPRTSSQQSKVGKKVEK
                     NNLQAGDLVFFHNPVSHVGLYVGNGEFLHAPQTGDVVKISKLSNRKDFNTARRVL"
     gene            33457..33789
                     /locus_tag="PC414_001426"
     CDS             33457..33789
                     /locus_tag="PC414_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 family protein"
                     /protein_id="HDF2463828.1"
                     /translation="MADPINEFIGIIRQEGKFHNEPSFFIGKIKSKLPDLKIEINNII
                     LEKEDILIDSWMLDRQIELFDTETSQEHKHEIKNPFIDTFESGNTVIMFKIGEKFAVV
                     SKLVSLDE"
     gene            33782..34216
                     /locus_tag="PC414_001427"
     CDS             33782..34216
                     /locus_tag="PC414_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HDF2463829.1"
                     /translation="MNNTIFPFMGVPEDYISPDNEELPIFKEFAWDFNKDEKIIENGD
                     FKIVERNEAIKVWIYKTIKTDKYVHLIYDWSYGTDIKNLIGQKYTKGLTESEAKRYIQ
                     EALLTNPYILEVNITNAEFEDDNLSISLNVKTIYGEEEITFV"
     gene            34209..35261
                     /locus_tag="PC414_001428"
     CDS             34209..35261
                     /locus_tag="PC414_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HDF2463830.1"
                     /translation="MYNGQSFSTLNNRTLNNINLPLYKGQGSSLYNIVSPINSELAQL
                     YIELSYIHKRVFIQDNFDDFLDRRVNEFGVYRKLGTEAIGEVTFEGKIGTQIPNGTII
                     SHSDLLFVIIKDITIDENSKLNVSPIQALEIGIKYNLSANTEFKLIEEITGVTKIYNE
                     LDLKGGTEIETDEELKERFYKIQKNQATSGNKAHYQSWALEVEGVYNAKVIPRWDGPG
                     TIKILIYGQNNQSVDNEVLQRCTEHIEEEKPIGPTVTIVTPSTFDINISATLTLENGY
                     DIESIKVVFLDIINSYLIENSREIVYIKVMSLLASIEGIHDIKNLLVNGDVKNIIVDE
                     EKVPAVSSAIFDIEVS"
     gene            35263..35871
                     /locus_tag="PC414_001429"
     CDS             35263..35871
                     /locus_tag="PC414_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HDF2463831.1"
                     /translation="MKLIDYLPSFANNEIDIQIQEALENELLTLIDEKDDLLEQFFID
                     TATWGLDDWENLLSIKVNYKLDFDTRRSNIKAKMRGKGTTTIEVIKAISEAYTKTNVD
                     VEVFSNLFSFTLSFITNNCSYNTILELDKKIEEIKPAHLEHKFERILFNKNELYTGAA
                     ISTGETVTIYPYVPRNLESFGEIAICSGNDRALEKVTLYPKK"
     gene            35888..36670
                     /locus_tag="PC414_001430"
     CDS             35888..36670
                     /locus_tag="PC414_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDF2463832.1"
                     /translation="MTEQQYFTLVTDIGKAAIANASITGEKVDFAKIKVGDGGGSSYT
                     PNESQTALKNVVWESTLEHAQGDKDNPNWVVIQKFIPGDVGGFEIREVGLFDSKDQLL
                     AISSYPTTYKPTADSGTVKELLIKVILVVSNVANINLKVDPTVILATLKDIQELDTKI
                     DTTKTELTSNIETAKTELNTKIGDTTQLTTTDKTNIVSALNEVKTSVDSIETTADKTS
                     IKDTDNLFESDNVEGALKEVMQEVKGNRSSIISTINSNLIPM"
     gene            37266..37754
                     /locus_tag="PC414_001431"
                     /pseudo
     CDS             37266..37754
                     /locus_tag="PC414_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893144.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            38156..38503
                     /locus_tag="PC414_001432"
     CDS             38156..38503
                     /locus_tag="PC414_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463833.1"
                     /translation="MNRNNRIIYDQTGKIWLQTGEAIGDIQELSEITELNFIDVEFGS
                     IDYSKQYIESINPVTKELNIKNIEVVLTDEQKRLEVLEKELNMLKEENKNRDSEIVNT
                     AFEVGNIKLNNNL"
     gene            38515..38685
                     /locus_tag="PC414_001433"
     CDS             38515..38685
                     /locus_tag="PC414_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463834.1"
                     /translation="MYNLLKLMIEQKNYSTKEDLQHKMDVFYAVNRITEEQYLELTGL
                     LNKEETPVEPTI"
     gene            38715..38996
                     /locus_tag="PC414_001434"
                     /pseudo
     CDS             38715..38996
                     /locus_tag="PC414_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889434.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            39304..39771
                     /locus_tag="PC414_001435"
     CDS             39304..39771
                     /locus_tag="PC414_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDF2463835.1"
                     /translation="MSKSYFNTKLGGSMNVTIVFLATNIFIKLVILAIAFDTLLGCLR
                     AIKTHQFNSSFGINGGIRKVAMIACIFFLAVVDILTKFNFLFMLPQDWVDFLRLNHLG
                     ISEFFSILFILYESVSILKNMYLCGLPVPKRLKEKIGSLLDTMTDELNVKGGK"
     gene            39775..40605
                     /locus_tag="PC414_001436"
     CDS             39775..40605
                     /locus_tag="PC414_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2463836.1"
                     /translation="MKICITVGHSILKSGACTSADGVVNEYQYNKSLAPVLADIFRKE
                     GHKVDVIICPEKQFKTKSEEKSYKIPRVNAGGYDLLIELHLNASDRQGKGSEVLYYSN
                     KGLEYATRICKKLGTVFKNRGAKLDKSLYILNSSKPTAILIESFFCDNKEDYEKAKKL
                     GYEGMAKLIVEGVLNKSIESNEVKQMYKHTIVYSGDDRVSADILGLYYKRAKESYLVI
                     DIKDYKPHRTQNLYVIGGVTCNKMKEMSKTTGEKFTQLYGNDVWSTMDKVIEFVKEKL
                     "
     gene            40677..41783
                     /locus_tag="PC414_001437"
     CDS             40677..41783
                     /locus_tag="PC414_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HDF2463837.1"
                     /translation="MLTFLDLFAGIGGFRLAMERAGHKCVGHCEYDKFANLSYKAMHN
                     PKEDEWFEKDIREIRAENIPKADVWCFGFPCQDISIAGNKLGFKGNRSSLFFSVTKLI
                     RELKEEDRPKYLLIENVKNLLSVNRGFDFAKLLIELDEIGYNAEWQNLNSKNFRVPQN
                     RDRIYIVGHLRRRSTREVFPITGTSRKTTLEQLNNPNHSSNRIYNSNGLSKCLLSKGG
                     NNTGLYRVGNINPSGNGAGGQVYSSEGFSPTLTTGKGESPKILIKENTKKGYIKASIG
                     DGIRLDHIEGKTGRGRVQHKKSCTLTTASNAGVIGKKFRVRRLTPKECFRLQGFPDDY
                     YDRAASVCSETQLYKQAGNSVTVDVVEEIAKKLK"
     gene            41921..42256
                     /locus_tag="PC414_001438"
                     /pseudo
     CDS             41921..42256
                     /locus_tag="PC414_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894425.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     gene            complement(42267..42782)
                     /locus_tag="PC414_001439"
     CDS             complement(42267..42782)
                     /locus_tag="PC414_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463838.1"
                     /translation="MTWSDLKEKNINEVVDCINNKLSEFESLKKVGDELGANESTIRK
                     WLNKKGYKRTGNKFVEDNKSYDDKYHIDSISSKIDDDIKYLSSEIDNIKNVLEWFKTK
                     DDSYHICVIKENDISIDLPDQSIKRTTVRINNTVWEMFNKFADENKHYDKHDLLSQLL
                     LESLLKYVKSR"
     gene            complement(42865..43770)
                     /locus_tag="PC414_001440"
     CDS             complement(42865..43770)
                     /locus_tag="PC414_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HDF2463839.1"
                     /translation="MSKLGIDIGNYAVKTSTDDIFESKVTEVKNFGSDSDSIKIGNKT
                     YYLGEGDEEINIVKYEKENFLPLLLGAICRNTDDEIVDLGLGLPVKQFAGLRKNLIEK
                     LQGKEYHVEFTRGNETTKRDITIRSIQTFPEGVTGYLYYAKDIVDQIAGRDVVLVDIG
                     GKTTDIALVQGNKATDPYSINVGTINIYDAIKKSLEMDERFLGKVEIKREKIQDYINK
                     GFYLNGEKQDIKKNIDASISLFKEIYNELKLNYPVSTAAVVVMGGGAKLLGEAFKKNI
                     PGIIVMSDVDKHVFANAKGYKKMMK"
     gene            complement(43790..43993)
                     /locus_tag="PC414_001441"
     CDS             complement(43790..43993)
                     /locus_tag="PC414_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463840.1"
                     /translation="MSRIVLTFKNNDKEKAIEKFLDEKLSATAYLKELVWEKMQEKKD
                     NAVVEQKKEIEDPANNFDFGSLE"
BASE COUNT        17781 a         5033 c         7497 g        13694 t
ORIGIN      
        1 ataataagaa atgatataaa attgtatcat tttaaagatt ttgtgatata ataaaagcaa
       61 gaagaactac aatctattta ggcgtagagt ggagttcata ataaacgtag tttatttttt
      121 gaatttaaat ataaatttaa attcaacctg taagtcactc tttgcacgag agtggctttt
      181 tgcttttttg aatagttcac tgattaagta aactaccaca ctagctgtta aactagctaa
      241 aacattaaac aaaaagttat ccatgtaact cacctcccct cgaatcgttg ggaggataat
      301 cttttgtaca tgaactccac tctatagatt gtatattacg ctcttcttgc taaatttaat
      361 tataccatat tttgtaatat cagttaagag tttgcactac aataatcctt cattactgct
      421 tatcttcttt aattttctcc aattcttatt tatagtaata tccttcttca tttactagcc
      481 cctgatcaaa taaatttctc ctaacagttt caagtttgac aggatctttg ctataatcaa
      541 tttctctttc ttctttttta acatcttcat catcccaata tttatctaat tcttccttaa
      601 actttaaatc atctttttct tgaattataa cttcacttat tttatttgaa agtgttgatt
      661 tgaaaaaatc ataattcttt gtatttataa tgtcagcatt atctctttca agtgctatag
      721 ctaaagattt atcaaaagcc tgtatataaa tatctttttt aaaatcaaat tctttaaaat
      781 caatcttaaa aagtcttatt gttcctttag taaatgcagt ttcatctggt atataaaatt
      841 ctattgtagt ttcagtttct ttactttctt cttgttcttc ataaattaca gtttccacaa
      901 catttttatc aaaatatttt cttttatcta aatctttaac agaaaactca atagctacta
      961 cttttctgct tttcttaatt tccttgaaat ctatatcaaa atattctgta gaattaagtt
     1021 cctgtatagc gggaacaata acacgtcttt ttaaatcatt atatttagaa tacttatctt
     1081 ctaataataa taattctttt aaaacatcca aagtatatgt aatcttattt tttgtccttg
     1141 tccataacct taataaatca taaaatcttt gcgcatatga atttcgtaaa ctaaaaaata
     1201 tttgtaaatt tattggagta tatccaactt gaatataatt ttctattaag tcaagtaact
     1261 cagcagaagt tttaacagta tatgttttat ctttttcatt ataaatccag tgatatataa
     1321 aactacattc tccccaacta tcatcatctt taatatatat tgttcctgtt cttaacttag
     1381 taaatatctt gcttattgct tgcggggttg tataattggg atttttcata agttctctaa
     1441 cttcttcaat agttattgtt atctctaaat ttttattttt ctgaaactca tataacaacc
     1501 ttacaaaaag tctattctct attgtttgca aatcatacct tgccataata aacttattag
     1561 atttcatcaa gatattgtct ttctggtctt ttacttgatt cattattttt ctcctaaata
     1621 gtatttacaa actaatcaaa agtttagttt atttattaaa taatataata tcaaaaccac
     1681 ttgcaaacgt caattttatt ttttgtcgca tttaattatc aaagtgtcgt actttaatta
     1741 tcaaagtgtc gcatttaatt atcaaagtgt cgtactttaa ttatcaaagt gtcgcattta
     1801 attatcaaag tgtcgcattt aattatcaaa gtgtcgcatt taaaacctta cataatagta
     1861 ttttcaatgt attcactatc gctaaatact attaaatact attaaagact atttattaaa
     1921 tactattaaa gactagtaaa gatacactaa tctattctta tcaaagtgtc gcatttaaaa
     1981 aataaaagtt catataaata aatttaaaaa tactgaatca ttttaattgt tcggaatata
     2041 tacgaattat ttgatacttg catattaaaa taggttttaa aaaagatata agtcatctga
     2101 aacgatttta agatgatttc ttaataattt atacagaaat aaatactaaa ctccttaaaa
     2161 tgatgacctt tcgtctctgc aacttagaaa tatcaatcat tacagaaacc aattttaaac
     2221 ctgcaattaa tggttttata aaaaacatag agttgagttt caaaaataaa aaaggtttat
     2281 cgaatttgta attttgatga tcggaaaaaa gaaattgata aaaataaagt tgattaagta
     2341 tagttttaag gcatttaaag gcttgtctga cacgttttaa ttgcaaactt gatattattt
     2401 acctaaaaag atacaaattt gcttaaaaat ggaaatagaa ggtcaaatat aaaaatagag
     2461 aaccaaataa aattatattg gctctctatt tatcttcttc ccaatcctca ataaactttt
     2521 ctaaactttt taatttattt gcagatattt cttcatcatc atgtaatgca cttataaaac
     2581 tttcaaagga atttctgtga aagaaactaa agaaactttt agtttcaact tttaaatatt
     2641 cttctttttc tattaatgct ttataatatg taaatctatc tttcttaaca acttctaaaa
     2701 atcctctacc aactaacctt cttaaaaaag ttaacatagt tgattttttc cattcatatt
     2761 tttcacctaa tgtttttaaa atttcagtag atgcaacttc ttcatcttca ctatcccaaa
     2821 tgtacatcat taccaaaagt tctgattctg gaatcttttt tatcttcata aagcacacca
     2881 ccttttcaaa agtataacta aaatactata acaatagagt taactataca atactaaatg
     2941 tctaaaaatg ttattccaat ttttaccaat taatttttct ttaaaaaaaa tttaagcagg
     3001 agtaaaaaaa ataaaaaatt atcaaaattt atcaatataa aaagaaatat aagtataaat
     3061 ttttctcttt gtatattgat tgtatacaat tctatttttg taaaattaaa ttttacaaaa
     3121 taatcaaaaa tggtaaattt tgacatttgc ctaatgtcaa attaggttta caattgtaac
     3181 ataaagaaga tatatatcga aataatacat ataaaataca tataaaacta cgtacgtaaa
     3241 atatttttaa aaataatcaa aaatcttaat tttcaatatt gaatacaatt aaattttcaa
     3301 actcgtctag ggcatgacat acaaaagcaa aaaacattaa tttagtatct gataaaaatt
     3361 taaaaattat aaaaggggga agtctgttat ttttcagaag caatgtaact gtaaaaatat
     3421 aaatcagaat attttgaaaa tatgggggat tctatgggag aattaaaact tgaatcaaaa
     3481 atgaattgca ctaaaaataa gaaaaaagtt gctatatcta tacatcaaat gaaaaaaatt
     3541 tctaaaaaag attatgtttt tttaacagat tgcatagaaa cattttatat gaaaataaaa
     3601 gaggtttgat aaattttcaa acctcttttt attgctattt gtttttcttt tgcaataatt
     3661 ctatcatttt caagatagca tctctatctt cttgttctaa ttcataaagt ttgttagtta
     3721 tttcttttac atcatcatca atttctaagt cagctaaaca atctttataa atttcacctt
     3781 ttccacttct tagccattcc tcatttactt caaattctct gcatatgtcg ttaatacttc
     3841 tttctgtcaa aactcttttt cctttttcca ataaagaaat ttgatcttgt gataagaaaa
     3901 ttctttcccc aaacgctctc tgcgaaagtc tttcaagagt tcttaactca tttaatcttt
     3961 ttccaatttc tttagctttt aatttttttg aaacttctgc catttttaca atcctcctct
     4021 gtcattacta tactataaaa acacgacttt gtcattaaaa aaataattat aacgacaaaa
     4081 tcatgataaa aataaaaaag atattgattt ttaacgacaa agtagttata cttaaaataa
     4141 gttgaacgac aaagtaagtg taggagggtg aaaaatgatt tgttcaaaca aagaaaaagt
     4201 agaaacagct ttgatgatag attatttaaa aaaaaacgaa ccaaatgaat ggagaactat
     4261 agaaaccata atctccagca catacgcatt aagtaatttc aaaaaagaaa ataaagacat
     4321 tatgaatcag aaataacaac agtaaatact taaagggagt ggtaagcttg aaaatttcag
     4381 attttaataa aaatcaaata ggaacagcag ttcaattaca agatataagg tttggtacaa
     4441 aagttgattg catcattacg aaagtagaaa agaatgaaat actagtcatg tattatgaaa
     4501 aagagacaga ggaaatagca tataaaactt tgactaaaga agatctgata cttgatgatt
     4561 ataaacttaa gttattatct taaaaaataa tttcggaggg gaggtgatga gatggatctc
     4621 ttgaaggatg caagaaaaaa agagtggttt tggctagaga atgatctagt agatagagaa
     4681 gatttaggaa tctatgaaaa aatgatttat atagttcttg caagatattc tgataatgaa
     4741 agttgttgct ttccaagcta taaaactatc gctttgaaat gcggatgtag taaaaggcaa
     4801 gccaaaagcg tagttaaaat tttagaaaat aaagggttaa ttaaaaaaga aaatagaata
     4861 aaaagtaact caaatgaaaa agaaagcaac atatattttg ttttaacagc taaattaggt
     4921 ggtgaatatg atgcacaaca ggtagtgaat atgatgcaca accctagtgc acctcatgca
     4981 caacaggtag tgcatgaggt gcacagtaaa aagacttata ataaaaagac ttatataaaa
     5041 agtaatacca ctacacaaaa agaatctaaa actgattatt tagacctatc attcttggac
     5101 ttagatatag aaaaagtaaa actaactaaa gatgaatatg acaaacttat aagcaagttt
     5161 gggaagaaat acatacacga taaaattgtt agtttagaaa actatatcat aaatggtaaa
     5221 gggagtaggt ataagagcca ctatagggct ttgctgactt gggggaatgg agatgctagt
     5281 aaaggtatag taccagaagt tacaaagact aagaatcctc ttagtggatt taaagaactt
     5341 taaaaactaa atagtaagga ggtagatagg atgaatgaaa aagaagagac tgtttatttg
     5401 aatagttcta atgtgaagtt attcttaggc gttagggata ttaaggaatt tgaggaattg
     5461 atagaaaatg tcaatgagcg aatttgtcaa ctccaagaat cgattaataa acttgcccaa
     5521 tttaatattg agttcgatat taagtcagat atttagttaa gtataaatta ggaggttgcc
     5581 tatgaaagat gaaaagttag aacttactga aaaagattta cactgtattg caagacattt
     5641 acaaaatgaa gttatggaga tggtgtttag agggaatata gaagccccta catcatgcga
     5701 agtttgcgat tacttgcaag aatgcaagga ctatttcacc catatagaca cttttataaa
     5761 attaagtgaa atgacaggtg tagatatttt tactaaataa atttagtaaa ttaaaatttt
     5821 agtattgtaa ttgacgtgct tttttaaaaa gaggacagtt gctattagca gtcattattt
     5881 gaattttgca aatgtaattt ttattacatt cgcattctaa aagttttgat ccagtgaatc
     5941 caggaaaagt tttatattca ccagaaaatt caactttatc gtcaataaga gagcattttt
     6001 ctcttaaggt tttaattttg cacatattca tcaccaccct ataattaatt tgaacatact
     6061 gtcaatatgc tccgattaat tataacatgc aaattatcat gatgtagaat taaagcatag
     6121 gtcaattaaa tttagttata aaatcaatga aacagataaa taattaaatg ttaaataaat
     6181 tagggggttg ttcatgaaag aggaaaagtt agaacttact gaaaaagaat tgcattgtat
     6241 tgcaagacat ttgcaaaatg aagttatgga gatggtgttt agagggaata tagaagcccc
     6301 cacatcatgt gaagtttgcg attacctgca agaatgtgag ggagatttta cctgtatgta
     6361 tcattctttt actaaattaa gcaaaataac aggtgtaaaa gtgactgcat ttaagtattg
     6421 ttttccagat gaaaatccca aagctactat ctaagaagtt tttttagaat aggacattct
     6481 tcattagaag aagagtcgca aatatgactt ccatcacatc tagttgacat taacttagat
     6541 ccaaaagtac ctggaggagc tgaatattcg caagtaagag aaatggtttc gtttattaca
     6601 ggacattctt tgcttataac tcgatattgg ctcatactct caccaccttt ctaattaatt
     6661 taaacatatt ggaagtatgt ttaaattaat tatagcatgt aaattatcat ggtgtagaat
     6721 agtttcaaat caaaagaagc aactaaatag ttaaagacag gaggtatgct aagtggaaag
     6781 aaaaaaacat gaatttacag atcaagatat acaaagactt attgaaggat taagaactga
     6841 tccaaaaata agagaaatga tatttaaaag taaaggttgt gaaactagaa caagtacaag
     6901 aagtaaatgc gaacaatttg ttgaagaaaa acttgcttat agaagttatg gatttgaaac
     6961 tccaaaatac tatgcaaaac ttattgccga cataattgat attttagttt acaaaaatgt
     7021 tccaagagat cgtatttgtg gggtattgta tgacgtcgaa agggtgattc ctttagttgt
     7081 gacattttaa taataagctg ggggtgatta aatgaattac tctttagaag cagagcaaaa
     7141 cattctagga agttttctta taaatgactc tataggttac aaaataaggg aattaaaaga
     7201 aaatgatttc tactttgagt cacataaaat tattttatct tgcatgaaga aaataattga
     7261 ctcaaataaa ccactagatc ttttgctttt aaaaaatgaa ttagaaaaaa tagatagatt
     7321 agtagatatt ggaggcgtta gttacattac cagtttaaca actatagtta taactacttc
     7381 aaacatagat cactatataa aaatcataaa agagaagtta cttaaaagac aaatttcaga
     7441 gttagcaaat gaattaatta ataattcaaa ttcagataca agtatagatg aattgattat
     7501 agatataaac gatttaaaag cacttgtgac gacgagcagt agtgtaaatg ataattacat
     7561 tgacgcatca aaaataaaac gtgaaaaagg cgtacacaag tccatagaca cgggttttaa
     7621 taaactaaac aatatgctag atggatttag atatggaact cttacaattt taactggtaa
     7681 acctgcatct ggtaaatcaa ctatagttaa ccagtttata gcacaagcaa ttacgaatgg
     7741 agagaaagca tttttgtatt ctggagaatt accctctttc atgtctatgg attggtttag
     7801 aaaaactgta gctaatgatt atcacataaa agaatataaa agtgtctatg gaggaacata
     7861 cacagatatt ccagattacg ccgttgagct tatatcagac tggatagaag ataaattttt
     7921 tctctatgat gaagatgcaa taagtgatga agtaaatcta ctaaatacca tagagcattt
     7981 atatttaaaa aaaggggtta ggttctttgt tcttgacaat ctaatgacaa taaaaactgg
     8041 caataaggca gataaatatg agagacaaga gcagatagta agcaacttaa aaaatctcgc
     8101 taaaaaatat aacttagtaa tagttttagt tgcacatcct cgtaaaaata tgggtgacat
     8161 gaagccaact atgtatgatg tgtctggagc aagtgagatt gttaattatg cagattatat
     8221 tttatcaact tacagagttg ttgatgaaga agaagagaca gacgacactt atttattaat
     8281 tttaaaaaat agaataacag gaaaacagaa tataagtttt aaaatgaatt tttcagaaag
     8341 aagaaaaaga ctttacacga ctagtgagga gttaaacaga gattacaaat atgatgtaaa
     8401 taaacaatat gttcaagtag aaattccaga agatgtattt taaaaaaagt ataaaaaata
     8461 ggaaatttta ttaagtaatg attttataag aaaaaccaat attgaataat ttaaaaattc
     8521 aatattgaaa aaaatcattt ttcaatattg aaaaacggat tttgaaaaag tattgaattt
     8581 caatactaaa aaaagtttga aaatatagca tttacaaggt ttatataaat taatttcctg
     8641 acaatgtaca cacaggttta gatatgctag caaaaaaact taaaagtagt gtaaggataa
     8701 aaaggctata taaatttata aaaagattta aaagtgttat taaattagag ctaaagttta
     8761 ttaattaatt tcctcgtaga aaaatattat tttaaataaa taaaggagtg attttacatg
     8821 atagcattag aaaaatttgc aggtggagtt ctaaaagaaa agtttaatac agagttacaa
     8881 aaagttttag ataacattgc agatccaaat acagatttta aaaaaacaag aaaaattagt
     8941 ttagagatag tgtttaaagc aaatgaagat agagatcttg ctgaagtaga tataaaatcc
     9001 aaagcaacta tagtagaagc aaaggctaca acaactaaag ttctgatagg aaaagattta
     9061 gagacaggaa gagttgaagc gtctgaattt aaaaatcaag tagcagggca gttggtcatg
     9121 gatattccag atagtgaaga aactaatgaa acagaagaaa atagtgctgg tgtaattgat
     9181 ttcagaaatg caagttcaaa gtagtttaag aaagtaagta aaatataaat ttaaataaaa
     9241 aaggagaata aaaaaaatga taagaaatgc aatggaatat ttagttaagt taggaaaaag
     9301 agaaacagta tgtgtagatg ggtttaagta cacaacagat gcattagaaa taataaaaga
     9361 accaaaagct aatgaattag aaataactac attgagtggt ttagtagatt atataaaaag
     9421 tggcatagat cataaggcag atgagttgtt attaatacaa gttctaactc caagaaaagt
     9481 gcttttaaaa tctcctttga gaaaaaatag agatagagag acatatattg aatgtgttgc
     9541 actactacca gagaaaagat ttgacagacc tttagactta gatagtttta acataatgct
     9601 acaatcagca tttataaaga ataatgatag agatacactt ttaaaaattg caggaaatat
     9661 ccaagaatct acagtaaaaa atgtgggaga tgatggagtt tctcagtctg taacaataaa
     9721 aacaggcgta gctagtgtat cagaagcaat agtcccaaac agagtaaagt taattcctta
     9781 tcgcactttc caagagatag agcaaccaga aagtgagttt attttcagaa tgagttctgg
     9841 accagcagca ggattatatg aagcagatgg tggagcatgg aaaaatcaag caatgttaaa
     9901 cattaaagcg tacttgcaag aagaattaaa agattttaaa aatgtaaata taattgctta
     9961 attgaaaatc taagagggct tacaataagt cctcttagta aaaaaggagg ggattagatg
    10021 aaaatgattt ctcatatgaa cattttagaa agagcagaat ttacagataa aatagctaat
    10081 gcaatcatgt ctgcaaataa agaaattgaa agaggctgtg cctttaatga agcagttgag
    10141 attgtaaaac aaatggaata tagagaaaat aaatacaatg aaggagatat tgcaatatga
    10201 ataatgtagt tttagttgga agattagcaa gagatccaga attaagatat ataccagagt
    10261 atggtactcc agttgctact tttgcattgg cagttgatag gggttataca aaaaaggatg
    10321 gaactagaga agtcgatttt ataccaattg aagttatggg aggatccgca gaattttgtg
    10381 ctaattatct cgcaaaagga agaatggttt caattcaagg ccaaataagg atagaaaaat
    10441 atgaaaaaga tggagagaaa aaaactttca caaaagtaag aacaaaaatt gttaatgcgc
    10501 ttgatcgcaa gccaaaggat gaagaaaaag aaattagttt tcaagtttta gatgatgaag
    10561 atattccatt ttaaattgga tggtgattat gtgagaagtt tagaggagag aaataaatta
    10621 gttgaagaaa atataaattt tgtatggttt attataagaa gatatttcaa agtatttatt
    10681 tcaaaatatc catatcttca agatgataca ttttcagaag gatgtattgg attatgtaaa
    10741 gcagcagatt attttgatag taaaaaaggt attgaattta ccacttttgc agctgtgtgt
    10801 ataaaaggtc aaatatttaa atttataagt aggtatgtta aaaaacatta cagagcagat
    10861 atagatagtt ttgaaaaatg catctataaa aatactcatg gtgaagaaat aagaatagaa
    10921 gatagattgg ctggtaatga aaatgtagat atagaaaatg ttcatgttat tagatcttgt
    10981 ataaaaagaa gtaaaataga agatatacag aaaataacat acttaaggga aaaaggatac
    11041 actcaaaaag aaataggcaa aatacttagg actaaccaaa tgacaatatg caggaagctt
    11101 ggaaaattaa aatctgaaat taatatacta ggaaaataat ataataattt aagttcattg
    11161 gaggcaaaat gaagactaaa ataattggtg gtagggaaaa tcagtgccca ctttgtaatg
    11221 gtagtgtatt taagattgaa acatcaattg gaatagtatg gcaatgtaag gattgtggat
    11281 gtatgtatca agatatgaat tgtaaagaaa acaaaaggag gcattaagta tggcttgtgt
    11341 tataaagtgt gatttttgtg gtgaaacaat agcagaaatt ctaaacataa aggtattcag
    11401 aattgatgat gaaggtaata taatgagtat cggaaaagat gtatgtgaca aatgttatga
    11461 cagattattt agaagtaact tgaataagaa gatgactaat tatgaaaaga taaaaaatat
    11521 gagcaaaata gaaatggctg aatttcttgc aaatggcgga agtggatgta ctagttgtgc
    11581 ttatgatttt caagaatgct taggcggatg tttagaaggt agaaaaaaat ggcttgaaag
    11641 tgaggcgatg aaataatgaa aaagacgtta ataatcgtag gagtagttat ttcaatatta
    11701 attatgtttg cttgcttgtt cttagtaact aaaaataaag caattgattt agaagagcag
    11761 gttcaatcag caactgcatc tgtagaggtg caagaaaaaa ggagaattga tttaattaag
    11821 aacatagtag actgtgtaaa agactatgct aaatatgaaa atagaacatt aaaagaagta
    11881 actgacatga gagtaagtgc aaatgatgaa tctatcaagg aagcaaaatt aaatatacaa
    11941 gcagtagcag agaagtaccc agatttaaaa gctaatgaaa attataagca actaatgagt
    12001 gagttgagta taacagaaaa tatgattgca caacatagag aaaattataa tatgcaagtt
    12061 cgcaattaca ataaatatat aagaaaattt ccagcaaatt taattctaaa tagtatggga
    12121 tatgaaaaaa tagaatctaa atatactgaa tataaagcaa aagaggatgc acctcaaaat
    12181 ttatttgatt aagaggtgca catatgaaat ttaaaacttt tgaaataact aagagagaat
    12241 ttttagtgag cataataatt gcttgcatga tgattgtgct tggatttttc attgatgata
    12301 aagttcaatc aaaattttta gaagataatg aaagatatta caaagcaact aaaataaata
    12361 atgacaaaga tctctttgat tatgctatgg aaacaagtgc gggagattct ttgataagtg
    12421 gaagatttaa aagtgtagat tctgtatcta tagaagaatt aaaaggggac tattggttta
    12481 tagaaagaac ggaagaaaaa tacactatgc atactagagt ggttaccact aaagacagta
    12541 aaggccattc acataaatat actgaaacat attggacttg ggattaccat gattcaatag
    12601 agaaaaaatg taataaattt aatttcatgg gtaaagagtt tgatgcgaaa gtaattacta
    12661 atttatctaa agagagattg gcattaaaga aagatatatt taaaaataat aatcttaaaa
    12721 taaaaggtaa ttatgcttac attaataata agagaaggta ctattatgaa gttataccga
    12781 aagaatttaa tggtactatg tatgctaatt taaaaaataa tactatagcg aatccagctg
    12841 ataaaaacat aaaagttttt aagggtgagt ctatacagaa tgttttagaa aataataatt
    12901 ttaatacagg gttttttaaa gtagtgtttt ggatcatctg gatattaata actacaatca
    12961 ttatatatgt attttattat ttaaaaaatg attggcttga agataaataa agatgtaaga
    13021 ggagaagatc aggtatgaaa tgtaaggtgt accatgctat taaaaatata aaagatgcaa
    13081 atgagttaat tgatagattt gaaacggaaa atatattctt tgaattaaat gacatagata
    13141 atgaaaatgt cttaaatgga gaaatgaatt taactgatat agaagatatt gcatcattgc
    13201 aatttatatt agatggagat ttatttatat ttagtagttg tgatgaaatg atatttatga
    13261 ataaatacac tcaaaaagag ttgggacttc aataaatgaa tgaggggaga tactaaagat
    13321 ggatttaaag ttaattagca gtatacctaa ttggaaggta gaagatcagt tggcaaaaat
    13381 tgatgaagag gtaactgagt ttaaaaatgc tatagataca ggaataacta aagatataat
    13441 tgcagaagga ttagatgttt gtcaaactat tttaacaatg tttcaagttt tagaaataga
    13501 aaactatatt actgaaggag tagagacaca taataagaaa ctaaatggaa gaggatggga
    13561 tctcaaagaa ataggagacg atatacttaa tttaattaaa aattcaatga ttaatgaatt
    13621 gattaatatg aacaagaaat taaaatgcct tgatgaaaaa gaatttaatg caaaaataca
    13681 atttttagat gtagataaac tatggaattt gtgcatgcct ttagttaact taagtactat
    13741 aaaaggagtt aatgaaagca ttgatgaaat gaaaatggga gagtttctaa aaaatattaa
    13801 ttaaagtaag gagataatat aatatgttga tagaagtaac tgaaaataat agagaattaa
    13861 aagtaggaga tattgttgaa attaatggtg gtaataagtt atatatgatt ctttctttaa
    13921 aggaaacaat aggtgggtat gttattataa atatgcagaa tggatatggt tcatttggtg
    13981 cttataaaag tttaagtcaa ctagaattag acttgaaggt aagaggttat aaattatata
    14041 gttctgatga ttacaagttg caattagtgc ctagagaata agatatattt caaaaaagaa
    14101 aaaaaggagc attacttcac gctcctgctt gtcaaaattc taaaaccgtc atcacaacat
    14161 cattataaca taattaggag tgtgaggtaa tgcaatctag taaaaaagac aatttgttta
    14221 gtagtgcaga aggtaagtta tatgattata aaaaaataaa agcggaaata gaaaaaatag
    14281 acattgatat gcagatgata aaaaacgact acagaggatg tagtggagtt gaaattaaag
    14341 aaaagacagg caagacatat gatattaaaa gtgtaattga aatagagact gaagaaaaag
    14401 aaaagaaaat tgccttaaaa gaaaaagaga agagacataa agagttgata atagaaaaaa
    14461 tagataatgc aatgaaaata ttaagtgaag aagaaaaaaa gatagtacag tataaatact
    14521 tttcaagtag caggacatct tgggaatatg ttggcagaat gattggtttt tcagcgagta
    14581 agtgtaagca aatgaggttt gatattatag ataagataaa aggattgcta taaaaatgtc
    14641 cgatttgtga cgattttaaa ccgatttatg tctgttttgt gtctaaaaaa tgtccggttt
    14701 gtgaccaatt tatgtctgtt ttgtgtcttt taatcatgat aagattgtat tgtgagaaaa
    14761 atatatttaa taaaaatggc ttggaattta cgttctaagt cttttttatt gtctagagag
    14821 gtgttgaaga ttaaacgtga tataacagaa gaaatttcaa gtgctgcata tgtaccagac
    14881 aatctaaagt attacgacaa tgtaatgaaa gaaactcctg gtatgtatgg aagagaatgt
    14941 tctttagatt ttataaagaa aaagcaagaa aaactcttga aactaaaaaa gaaggttaag
    15001 aaaaattatg attctaatat taataagata gactcatatc taaaaatttt agagaaaagt
    15061 ataattgatg agtctgatca cgttgagttg gttgtattta aattatattt gcaattagaa
    15121 aatgttgtaa aagtaactga aactgtaaac gacttaggat ttagaataaa gactaacact
    15181 tatgcaagag aaagaaaata tagtacaaat gatataacct ctataatcac agatcctttt
    15241 actgatgtag ttgaggactt aaaaatatta gttcaagaga ggcaaagaaa aaattattat
    15301 ggaaataagg agtgttttta atgaatggaa gcacatagtg gacaaaaggt taatcccata
    15361 aaagatatag aggatgtatt tagactttta aattttctag aagaatggaa tgaaagaaac
    15421 tatttattag ccctctttgg aatgtgtact ggacttaggg taggggatat attagcactt
    15481 aaagttgcag atgtaacaga cttaaaactc gataaaaaag gaaataaaat aagagtatct
    15541 aaggattgga ttagagttat cgaagaaaaa agagactata atagagaggt ttttttatct
    15601 gatgtaataa aaagtgctat agaaaattat actcaagata aaccaggaga agaattttta
    15661 tttaaaagtg gcaagaggaa gaaatttaat agacctatcc aaacaagaca agcaggaaga
    15721 ataataaaag gagcagcaaa aaaggtagga ataaaagaaa atgttgcaac acactcttta
    15781 agaaaaacat ttgcaaggca tatttatgat gaagaagaga ataagactta tgcattagaa
    15841 cttataagga agattctagg acataagaca atagaaatga ctagaagata tataggaata
    15901 gataaggatg aagaagtaaa agcaataact aaatttacta ataagttaaa gaaaagaaaa
    15961 agaaattaaa atatcttgta ttttatctaa aatttaatat gttacgtttt tcaacaacag
    16021 gacattatta gaatgcaaag agccttaaca atagagttta caaggaatag aatagattta
    16081 ttaaaatgtc tgaaaatata tattaaaggt catttattca agggatttat agggatgtag
    16141 atatttaaat gtttatttag aagctgagta aagaggtgat taattggatg tgaatgaagt
    16201 tgaaataatc gatagtagct atgaaaaagg taaagagttg acagaagatc agtgcaatat
    16261 ggtaactcta ttgattacag gtgctacagt aacagaaact gccagaatta ttggagtaaa
    16321 tagaaaaaca atctataatt ggatgaataa agagcatgtt aggaaagaaa tggacagacg
    16381 gaaacaggag ctaacaaacc aaggaaattt aatgatatta aaggatttag atagttatat
    16441 caacaatatc aaggaattag ctagtgacaa gagcgataag agggttatgt tggcagccaa
    16501 tcagtatctt ataaatcgta tatatggaac tcctacaagt acagttatac aagcagagga
    16561 taatagtgtt ggcatgggta tggatgcaac agagatagaa gcagctatag ctaagataag
    16621 aataagaaca agcaagaagt aattagatat gaaataaatc ctgcaagaat atgttttaat
    16681 tatgtttgaa atcaattaaa gcaaaacata tattatatat atatttactt taataattaa
    16741 atcatatatt gtatttaact ttagttatag ctaatttaat aaaatttagt gagacttaat
    16801 aattaaagtt tcatacagtg aagtgatgtg gattattata ggatatgtga acctattgga
    16861 aatgctaggt tctttttaac tatgccgaaa atacatattt aaggaatagt tgaaagataa
    16921 ttaataattc taatcaaaaa aatatttctt gcaagaaaat aattcaaaat actttaatat
    16981 atttattttt tttattaata gggggtgtcc ttctattttg aaaaaattat tttttatgga
    17041 aaatgagttc tagaaatttt ctagcaaaat ttaaaaacca gagatgtatt tcaaagaggg
    17101 tgattttatt agagaaaaat ttacattgtt tattttatta attgcattac tcactgagcc
    17161 taaaaaaata gaagaagtaa gaggaagtaa atacgagtgg ataatctaga aagaaatata
    17221 caattaatat ttagttatct agcaaagact tttataagaa atggagcatc tataaaagat
    17281 gctgaaaaag aagcaaatga gattataaaa gccaattcaa acaatctttg gggagttaat
    17341 ggtctcgctt atcaacttgg aaaaataaac ctagaatttt tctgtatgta ttttatgcaa
    17401 gacacatatt taccaaaaga agataatgct gctgctccta tagctaaagt tcatcatgag
    17461 ctatgggaag atatacaaga atctataatt ggagatggtt cacagcaatt aggaagaatt
    17521 tatccaagag ggacaggtaa aagtgcattt ggagatttag cgacaactgt atggtcgcat
    17581 tgctataaac ataagacata tactttgatt tgctctgaca taggatctac agcagagaaa
    17641 tttgtaaaag atataaaaaa tgcattactt gaaaatgagt atattaaaaa agcatttggt
    17701 gtccttttaa atgataatga tagaaaatac atttgtaata gtactcaact agagctaaca
    17761 aataaaactt ttattgaagc tatatcatct tcatcaccca tgagaggaag aaaatacaat
    17821 aataaccgtc cagatcttat catacttgat gattatcagt cagaagaaaa tgttagaaca
    17881 gaagatgcta gagagaaaaa atttaaaaga ttttctgatg atgtaaaata tgctgctcaa
    17941 aaaccagtca taagaaatgg taaaactata aaaagaggaa caacttttat agcattagga
    18001 accttacaac acaaagaatg tttttatagt agactaaaaa atttaccaac ttggaagttt
    18061 aaatgcgaaa aaggagtttt agttgataat gtagatgaat tatttaattc tggtttatgg
    18121 cttaagttca aagagatctt atttgatttt aaaaatacaa atcatcttga agatgctaaa
    18181 gaattttact gggaaaatga aaaagaaatg aaattccata tattatggcc aagtttttgg
    18241 gattgtttag atatggctct aagttattat gaaaatccaa catcttttaa gcaggaagtg
    18301 caaaatgatg tcaattctat aggagaaaaa tggtttaaaa ctgttagaac agaaactaga
    18361 gaagaaattg aatcgcatac ttttaaaaag acaatgctgt tatgtgatcc agcatctgcg
    18421 ggtgggtcga aacatgacta tagcgctttt cttgttggaa gtgaatcaga aaatggttta
    18481 ctatatggaa gattggcaga actagctaaa ataaatgcta gaactgattt tgataaatat
    18541 atagaccata tgatttattt attaaaagtg tattcagata taacacatat ttatatagaa
    18601 aaaaatacat ttaatggcgc agatgccaac caattagagt ttaaaataaa aaatgataat
    18661 gttcttagtt atagagatat agaaatcatt aatgagcaac aaaagaaaaa taaagatgat
    18721 aaaatctcta ctttaatacc tgttttaagc aaaggccaaa tgatttttgc agaagaagat
    18781 aaggaattta tacagcaaat acttgatttt acaggacaaa aatactctct acatgatgat
    18841 gctccggata tatcatcgga atttattaat cgaattaaca acataaaagt ttctgagaca
    18901 gtaaatatct ttgatagaaa attattaggt ttttagttgg gaggtaattt attggctaat
    18961 agatcaaagt ttgaagaagt tcaaaaaact ttaactaaaa aaggaaagaa gttcaatgtt
    19021 ggaataggaa aagatgaaaa aggatatttt gcatatactc atagagcaag gagtaaaagt
    19081 tatatttcaa agcaagatat tccaataaaa gttttaaaat ttatagagtc tacaggatag
    19141 gatgtgttgt taatggaatt agatttagac ttaataaaag acatatatga cgattggaac
    19201 tctaataaat ccgaatatga tactatgtat aagtattata aaggtgaaac ggatgctatt
    19261 agcaactata aaatggttac caagagatct aataataaga taaacactaa tttcttaaaa
    19321 aagtttatta atgaagaagt cgcttattct cttgcaaata agattaccta tacaagtaga
    19381 tctggtgatg agaagattat aaatgattta gaatactata cttgccattg gagtaaaaaa
    19441 catgactcag atttattgag gtatgcacta ttatttgggc tttgctatga actttactat
    19501 gtaaaagatg atgaaataca agtaaagata atcaagccaa cagatggctg tcattatgaa
    19561 aatgaaaatg gagaaatagt atgctttatt agagagttta ccaaaggttt cgaagatgac
    19621 acgtatgtag atgtatatga caaagaatat atatatcatt ttgattcaaa tttcaaagaa
    19681 gttgaaagtc ctacagtaaa caatatattt gatggaaatg ttccaatatc aatttgcaaa
    19741 agaagtgaag aactaggaaa aaatacaata tttaatgata taaaagggtt acaagatgct
    19801 tatgaaacta atttaagtga tataagtaat gaaattagtg attttagaaa tgcttattta
    19861 accttttctg gatgtaatat taaagaagat gatttgccaa ggatgaaaga acttggaata
    19921 cttcaagtta atggagatgg aaaaatagag tggcttatta aagatatgaa tgatacattt
    19981 gttcaaaata cactatcttc aataaaagaa aatatgtatg aaattacatc tcatataaat
    20041 cacaatgaaa aaatgcaaag taatacatca agtctagcag ttatagcaag attaatcagt
    20101 acagaatgga tttgtagcca aaataatgat agtattgcag atactttatt taataggtac
    20161 aaattattgt gcatttggct taataaaaaa tatggttttg actatgatta taaagatatt
    20221 aaggctaaat ttacccctaa gataccacgt gatgatttag ttgtagcaaa tatattaagc
    20281 caacttggag ataaattatc tactgaaact ggcttatctc aactaagttt cattgataat
    20341 cctcatgctg aaatagaaaa agctaaaaaa gaacaggaaa tagtttcaga gggagagatt
    20401 ttattagatg aatcaaaaga ttataactaa attgactgaa gatatatata aatctgctga
    20461 aaataggaca aaaccatttt ataagtatca aagagaaaat agggataaac ttttattaga
    20521 aatagcaaag atattgctaa cacacgatat tattaatgaa tacttaaata ttaataataa
    20581 agataagaaa aatctaaggt caaaacttaa taaaattata aatgagtatt ctaatagtta
    20641 tgatgaagaa gtcaaggata tagaaactat tttatctgat tcatctaaga ataaatattc
    20701 aaatttgata attttattag gagtaatact gaagataaaa gatgttagta atataaacaa
    20761 taaagttata aataagatag tgaataataa ggttgatgga aaacattgga gcgatagact
    20821 ctggaagaat aaaaaaggta tagaagagtc cttaaaaaaa gaaataaaga gttttctaaa
    20881 tggaaaaact tctgtaaata agatagaaaa agcaataaga gataaatatt caacaggtgc
    20941 aggacaaacc agcagactaa tagaaaatga agttgccaga tgtcaaagtg aagtaaatga
    21001 gtattttttc aaggttcaag ggattataaa agtaatgtat tgtgcagatt tagattctcg
    21061 cacatgtagt gattgttcta tgcataatgg agtgatattt tatgtcaatg aagataggcc
    21121 ctctttgcca agacactgtc actgtagatg tcaatatata cctgtttaac atgtattaat
    21181 taatttaata ggtgttttta ttatgtctaa aaaatatgag gtgaaatatg aaagttgaaa
    21241 tattaggaac agaatatcta gtgatagaga aacttgaaag cgaagatgtg ctattaaagg
    21301 aaagagctgg atattgtgat cactctgtta aggaaattgt tatagagaaa ataaattctg
    21361 aagaaggttc attgaaagat ttaagtgtat ataaaaatga agttgctagg catgaaataa
    21421 ttcatgcatt tcttagtgaa agtggattga agagttgtag ttcttgggct acaaacgaag
    21481 aaatgattga cttctttgca attcaatttc ctaaaatagt aaaggttatg gataaaatag
    21541 aatgtttgaa ttaaattttc aaaaaggaga actttataca tgttaaaaaa agaagtgttg
    21601 gaattactaa aagaaataga agatgatgca gaaatagatt cattgctgca atcaacagat
    21661 ttattcaaat ctgcatctga taaaaagctt acaatagaag agtttagaga attaattaat
    21721 aatgatgcaa actttaaagc tattatagat aatgaaaaaa ataaatatca cagcgaagca
    21781 cttgaaaact ttaagaaaaa agatatgcag acacttataa gtgctgaagt tctaaaaaga
    21841 actggagcaa atgaaacaga agagcaaaaa gcaataaggg aattaagaga aagcctagac
    21901 aaactaaaaa aagaaaagca gtacgcagaa aaagtttcta aatataaaga tattttagtt
    21961 gaaaagaaaa tacctacaaa cttaattgaa tatctattat ctgatgatga tgataaaaca
    22021 aatgcaaata tagagatatt tgaaaattca atgaagcaat atgtgcagtc aaaggtagat
    22081 gaaagaatta aagatggttc atacgttcca cctggcaaag atagtgcagg ttcgctatca
    22141 gaaattagaa aacaaataaa gcaagggcta aatagtcttt agtataaaaa aagaaaggat
    22201 gattttataa atggctaatg tattacaata tgtaacactt tttcaacaag aactagatat
    22261 gcaggtaaca gttggctcaa ctactggatg gatggagaaa aatgctggtc aagttatata
    22321 taatggagga agagaggtaa aagtaccaaa agtaacaatg gatggcttag gtgattatga
    22381 cagatctaaa ggatatacgc aaggcgcagt tacacttgag tacgaaacaa tgaaaatgga
    22441 gatggataga ggtaggactt ttatgttaga ttcaatggat gtaaatgaaa gtaacttcgt
    22501 tataaatgca tcttctgtaa tgggattatt tcaaaagcaa catgttatac cagaaataga
    22561 tgcatataga tactcaaaga tattttctga aacagctaaa gtagatgagc gtgtaaaata
    22621 ttcatacgaa gctacagtaa ctacaatatt aagtgaattg aaaaatcaac ttgcatcaat
    22681 acaagattta tctggagata tacctcttat tattagtatg tctagcataa ttgcaagcat
    22741 attagaacaa tcatctcaac ttgatgatat tgaatttggc gatggagata taaaaagaat
    22801 tgtaaagtct atagacaatg tacctataat aaaagttcca tcaaaaagaa tgaaaacaga
    22861 atacgtattt agagatggta aaacatctgg acaaacagag ggtgggttta ctgaaggagc
    22921 aaaggcttta gatataaact ggttaataac tccagcatct gctccaattg cagtatctaa
    22981 gactgataag ccaagaatat ttactccaga tcaaaaccaa acagcagatg cttatcaaat
    23041 agactataga aaatatcacg atttgtggat attggatgaa tatttaaaac tttgtagagc
    23101 atctgtgaga caagctaaag ctgcacaaga ataaggagtt gttttaaatg tttgcattga
    23161 taaaaaataa cataattaaa gttgttgaag atgaaaattc gagagataaa ttgatttcaa
    23221 aaggatttaa aatatattca gaagaagtga ctaaagagaa tattgagaaa aaagaagaaa
    23281 aagttaaatc aacctctaat gatttattta atctatctaa accagagctt atggaaattg
    23341 ctaaaggcaa gaatataaag ggatattcaa ctaaaactaa agaggaatta attcaactac
    23401 tagatgagta caatgcttga taatgttaaa ttagttttag gaatagaaaa tgatagatat
    23461 gacaaattaa taatccttta tataaataaa ataagtgaga tggttttaga atattgttct
    23521 aggcaggaac taagtacagc actagaaggg tttgtagaag aaaaagtaat atcaatatta
    23581 tcttcaagat ttgaagaact taagaaaaat agtggacttg ataatctaga aaaaagctta
    23641 aaagctataa ctagaggtga cactaagata gaatataatg ctttaacatt acaaactgct
    23701 gaaaatagca tcagttcatc atctttttta actgatactg ataaaaaatt tctaagccca
    23761 ttttgtattg ttagtatgta ctaaggggtg attaaatgac tgaagcagat atattagaaa
    23821 tgacatacct tgataaaatg actataatta gaaaattaaa aggctggaat gaagaaacat
    23881 gttctaatga ttttagtaat gttgttatag ctgaaaatat cccatgtgca atttctagaa
    23941 aagaagaagc cattgtaaat ggtgatattg gatctatagt tgtaaataga aagttatttt
    24001 gtagaccaga agtagatata aaaataggtg atactataaa tacaacctat aatcatggag
    24061 aagaagctgt atttaaggtt tctaaggcaa atatatatcc aagccatatg gaaataccta
    24121 ttacagaatt ggagaggata taatgggaat agataattta ggagtctttg aagcattagc
    24181 cttagattta ggtctaaatg catcaaactt taatagaaaa atcaagaagt ttacaaaaga
    24241 aactgggcaa gaactagcag aaaatataag agatgaaact cctgtaaaaa ctgaaaagtt
    24301 aaaaaaggga tgggaagaaa agcaaccaga atatgatact tatattgtta aaaatgaagt
    24361 tgattatgct actttagtaa ataatggcca ttcaactaaa aatggtggca ttgtccctgg
    24421 ttcttttatg gttgaaaagg gaattgaaag aacaaaagaa aatatgacaa gtgcaaaagc
    24481 aaatttattt gattggtagg tgattagttg cttacaaaca gagacattat aatagcagtt
    24541 aataaaagaa tcgaaaatga gtttagatat atagatattt ttattaatga ggataatata
    24601 caaggctttg aggaggcttg tttttttgtg caacttttac ctgtttctaa taatattgca
    24661 aataaagttt tagatactaa aaagatattc atagatattg aatattacca aaaggctaga
    24721 aaaaataaat taaatctata cgacatacaa agtagattag aaaaaatatt taacagaaac
    24781 attagagtta aagatagaag tttaatcatt gagaatatat ctccatcaat cgcaaaagat
    24841 cctgtaggat ataaactaac ttttctaatg actgtatctt attttgaaga agtctatttt
    24901 gaaaatgaaa catttgaaat tatggaaagt gtagatttag agataggaag tgattaaatg
    24961 ggtttaccag aaattaatat ttcttttatc caagcaggaa aaacttttgt taaacgctct
    25021 ggtggtattg tagcgcttat tttaaaagat acaaaaaata tagggttagt tgagataaaa
    25081 gaaatagagg atattccaga aaattattct gctgctaatt tagattatat aaaaatggct
    25141 atgaagggaa atgtatatcc tccaaataaa gtattagttt atacattaga tacagatgaa
    25201 tcaatagatg atgcactaga ttttcttgaa acttgtgagt ttaattatct ttgtatgcca
    25261 gatgagacag aacaagattt gcctaaaatc aaaacgtgga ttaagaaaat gagagaagat
    25321 aataaaataa aggttaaagc tattactgca agtaattcag cggattatga aggtataata
    25381 aactttacag caactgatat tgaagttgaa ggtaaaaaat acacttcaaa tgaatttctg
    25441 ccaagaatag caggttttat tgcaggaact ccatcaactc aatctgtaac atatgcagaa
    25501 ataccagaag ttactaatat acctaagcta acaagagctg aagcaaatac aaggataaat
    25561 aatggagagc taattttaat aaaagaaagt ggagctatag taatcgctag aggagttaca
    25621 tcctttacaa caattactga ttcaaaaggt gatatattca aaaagattaa gttggttgat
    25681 acactagatc aaattcataa cgatattaaa aaaataattg taaaaaatta tataggaaaa
    25741 acccaaaaca cttatgataa taagtgttta cttattgttg caatacaatt atatcttcaa
    25801 gaattagaaa aagatggttt aattgatgaa ggttcaactg tagaaattaa tttagatgct
    25861 caaagagcat ggctaaaaaa acaaaatata gatatatcta atatggaaga acaacagata
    25921 aaagaataca atactgacac tcttgtattt ttaaaagcaa gaattaaatt aatagatgca
    25981 atggaagatg tatatttaga catctctatg tagtttggag gttaaaattt gaaagctaaa
    26041 atagatgcaa aaagaataat atctggtact tttggagagt gttggctaga taataagcaa
    26101 cttacatcaa caaaagggat ggaggccaag gttgatatag ataaagaaga aataaaatta
    26161 tgtggtcaaa taatgaccac atataaaatg attggtgcaa ctggaaaagg atctataact
    26221 ctttataaga taagtacctt atttggcgag tatgtcgaaa aaatgttaaa tgaaggtaaa
    26281 gaatttaaag taactataat aagtaaattg caagacccag attcatatgg ttctgagaga
    26341 attgcacttt atggatgtat gtttgacgac ttaactttgg ctgattggga agctgcgaaa
    26401 gttggagaaa tagaagttcc attcacattt gaaggctttg aatacttaga taaaataaaa
    26461 gaaaaatagg aggattttat tatatgaaca cagtagaaaa attattaaat atggatgcag
    26521 gtaaattaaa aatgccatct agcgcatttg aattatattg taaaaaacta gatgatactt
    26581 tagaaataga gtgcaaggct attgatccag aacgttttga cgaaataaga atgaactcta
    26641 tggacgttaa tagtggagat gtagaaaaca ttaacatata tgaacttaaa gtaaatacaa
    26701 tattagaatc atgttctata tttagcaata tggaattagt taaaaaattt agtgcaatta
    26761 cacctaaaga attaattaaa aagctattac ttagtggtga aatagataaa ttatacgagg
    26821 aggttaataa agtaaatgga atagatgcag agggagataa aaaaagacaa aaagaaagag
    26881 ctaaagatat aaaaaactaa ttaaaacgga tggagaagtt aacttgatgt acttattatt
    26941 taagtataaa ggtataatgc catccgtttc ttatcatata ctaagaggcg aaaaagaaat
    27001 tgttagtgtt tttatggatc aagaattaga tgaaagaaaa gaagaaataa aatctggtgt
    27061 tgggaggttg ttttagtata tgagttctaa tacaaaggtc ctacaagcaa taataaaaat
    27121 gagagatgaa gcaagttcaa cattaaaaag agtagcagca aatactagag ctctacatga
    27181 ttcatcttcc caagcatcaa gagcattaaa taatgttcaa agtaatttaa aaagtgttgg
    27241 aactagtgct ttaaaagcag gaggtcttat cactgctgga gttggtggtg catttgcagc
    27301 tgcaactaaa gttggaacag aatttgaaag cgctatgtcg caagttaagg ctacatttgg
    27361 aaattcctta aaaccaggag attttgaagc tctgtctaag gctgctagag atgctggaaa
    27421 agctacaact aaaactgctg ctgattctgc taatgcactt cagtatatgg ggcttgctgg
    27481 atgggatgtt caaaagtcaa tgcatgcctt aatgccagtg cttaatttat cagaagcagc
    27541 ctctatggat cttggtctag catcagattt agtaactgat agcatgagtt ccatgtgctt
    27601 agaagttgat aaggtcagta aaagtggaaa agtactagct ggtgcaggtt taagtgagta
    27661 tttagataaa gttgcaaatg catctactaa atcaaataca agtatacaac agttgatgga
    27721 agcgtttaat gtcgcaggtg gtacatttag caatttaaaa gttccacttt cagaagcaac
    27781 tgctatcatg ggaatacttg caaatagagg agttaagggt acagatgcag ggcatgcttt
    27841 aaactctatc atggtaaatc ttacaacagg tgcaggtcaa gcaggtaagg caatgaaaaa
    27901 gttaggtgta agtgcatttg ataatcaagg taaatttaaa ggtatgtcaa atgtgcttaa
    27961 agaggttgca aataaaacta aaggtatgac tgatgaacag aaaaattatt acttatctgc
    28021 tattggtgga aagactcaat taaaaacgtt acaaaagtta cttgatggtg tgggaaatga
    28081 atatgatagc ttaaagaaaa aaatagataa ttcaaaaggt gctttaaaaa caatgtctga
    28141 tgtaatgaag gataatacta agggtggact tgcaacctta aaatcagccc ttgaagaaac
    28201 tggaattact atttactatc aactaaagcc agcaatagca tctgtagttg ataatttaac
    28261 taaaatgact gtttggttta ataatctaag tgaaccaata aaaagagtca taactaatgt
    28321 aatgctttca gttggagcat ttggagtact tttattaact ataggggctg tgtctttaat
    28381 tgtttcaaaa gctataggta tatttggctt ttttgttcca ataatattaa gagcaaaagt
    28441 tgctttcaga gcttttgtaa ttggtatgaa accattaagt gcagtatttt tagctgtaat
    28501 gggtcctgtg ggatggacta taacagctgt aattgcatta tcagtagcat ttaccattgc
    28561 ttatgctaaa tctgaagtat ttagaaataa ggttaattca ttagtaaaat catttacaga
    28621 atttgcaaag cctatagtaa atttttgtat gcctgcactt aaacagctag gagagaaatt
    28681 taaagagttg ttatcagcat ttggtccact atttgcatct attttaaacc taggaagtgt
    28741 aataatgaag gtactaggac ctgttttaat cggcgtaata ggttttcata tagcaaaatt
    28801 agttttgatt ttatctactc tggcaactgc ttttaaagct gtagttgata ctataactaa
    28861 tgtattaaat ggtctagcat ctattttaaa tggagtattt gatttaatta taggtattgt
    28921 cacaggcgat agtaaaaaga tgatggatgg agttaaacaa atatttgatg gtggaactaa
    28981 aattataaaa tcaatttgga aaggtttcat agattttgta actgctccta tacaagctat
    29041 agctgatata gctgacagct tatttaaaga taaagctaaa tggcttacag ataagtggag
    29101 agatttaaga acattcttag aaaatccaat tgaagcaact gccaaaatat ctactaaaac
    29161 aaatgtaaat atcccaggaa gtaaagcagc agatgcagct ggtaaatcta caccaaatac
    29221 aggtggatta aaacaaacag tagcaaatct aaaaggttca tttagtgatt ttaaaacttt
    29281 tattaagcct atgacagact ctataagtca aggagttgaa tctggaaaaa ctaaatttac
    29341 agaactgcaa aataaaatat taaatgatgt taaacctgca ctaaaggtcc tagaggattc
    29401 tggagtaaat tttaaggata agtttaagga gtcaataact tcaataaaag ataaatttaa
    29461 tgaattaaag aacatattag ctggaaatat taaaacaaat atagataatt tagtaaaagc
    29521 atttgaacct ttaaaacctc atttagataa tttgaaagat tgctttgata aggttaaaaa
    29581 agcgattact gattttttta ctccagccaa acaggttgga gatacagtaa acaaagtaag
    29641 ccaaccaatg gatagtgtta aaaagtctac tgatggaatt aaactttctt tccaaagttt
    29701 aactcaagca cttgctcctg ttaaagcttg gtttcaagga tttatgcctt tcctatcaca
    29761 aataggacaa caacttttac catctatagg aattgcaata gctggtgttg ttggcggaat
    29821 aattttagca tttacatggg ttttaaatac attgaccgca ataataacaa cagtatctgg
    29881 aatcataaat ggaattatgt cttttatatc tggaattatt aatgttatta tggggcttat
    29941 aacaggaaat gctgatcaaa ctatggatgg acttaaacaa atatttgata gtggagtagg
    30001 tgtaataaaa tcaatttgga atggccttgt tgatctagtt agtggcgtac tagaacctgt
    30061 aatagatctt gctgattctc ttttcaaaga taaagttaaa ggggtaaaag aagcttggaa
    30121 taaacttaaa gatttcttta aacatccaat taagggaaca atagaaatat ttaaaaaagg
    30181 tcctagttta caaattggaa aaggtgcaga tgctgctggc aaaagtccaa gaaaagcatt
    30241 tggtataaat agagttccta gaaatgatta tttaataaga gcacacgaag gcgagaaact
    30301 tttaaccaaa caagaagcta atcaatacaa aaggaatagt tcaaatggaa gtttatttct
    30361 tgataaattg gctgatacaa taattgtaag agaagaggct gacatagata aaattgtaag
    30421 aaaaataaac aaaaaaatat ctcttgcaaa aattggaggt gttttgtaaa ttatcgtgct
    30481 ataattgtag tatataattt acagggggtt atgttatgaa taaaaaaata ctagtattaa
    30541 caatatcaat tatactatct ttaagtttag ttggatgcag cacaaataat tcaaaaaata
    30601 gagatagtaa atctaagcaa aacacagagg aagttaaaaa tcataaaata ggcgaagttg
    30661 ttgatttaga tggagcaaaa gtatctgtta aaaatattag gaaattagaa acaaatagta
    30721 atgattatgc tgttgaaatt gaaataacta atacaggtga taatgattta caaagtgatt
    30781 atctagaaaa ctatatatta aaatcaagtg acaatactac tggagaagca ataaataacc
    30841 aagaattaaa tggagagtta atgtttgata caatacgccc aggagatact ttgaaaggtg
    30901 aaatagcatt cgaacttaca aaagatgcaa caccaaaaac acttgaaatt gcttatgaaa
    30961 atgtaggcaa atatacagta tttgatttat agtaattatc ttaaaagacg cttatttact
    31021 taagtgtctt ttattttagg aggagtttaa tggaaatatg gttaagacaa gcaaataata
    31081 catttaggtt tcctgttatt ccaccctctt ttgagataaa tggaagtgcg acaataaata
    31141 catctaatat tttaagtgtt ggagatatcg cagtatttgg aggattaggg cttaaaacta
    31201 tagaactatc ctcttttttc cctaatcaag aatatagttt ctgtaattat aatggatttc
    31261 caaaaccata tgattgtgta aatttaatag aaagttggat gaaagaagga tatatattaa
    31321 gatttatcat tacggaaact aatataaact ttgaatgtat aattgcagat tttaattata
    31381 gagaacaaga ttattcaaga gatatttatt ttaccttaag tctaaaagaa tacagaagga
    31441 tacaaatatc caaagtaaac attaataatg atgaaaaatt atcttctgaa aagaatgttc
    31501 ctctaacaaa aggatttgat actaagcaaa aaacgcataa ggttgttgaa ggagatactc
    31561 tttttaagat agctaaaaaa tattatagca atggagattt atgggagaag atttataaag
    31621 caaatgaaga tgagattaaa gatccatctg taataaaaaa tggttgggta ttaataattc
    31681 cttaaaaatg gtggtgattt ataattaata aaatcaaaat acaagttcat ataaaaaacg
    31741 gaaatatata taatgtaact gatttagtgg ataaatgcac ttggtctggt gattatagat
    31801 caccatcaag aactttggaa ttttctataa ttcaatctgc aagtgatatt aattttaggc
    31861 agatagatat tccagtagca agtacagttt gtttctatgt agatgataaa gaacttttta
    31921 gaggaatgat aatagataga tctaaagact ctagcaataa taatatagat tttacagcta
    31981 aagatatggg ttttttactt ttacaaagcg aagtgtcata caattttaaa gataaattag
    32041 tcgaagatat tgcaaaacag gtttttgtag ataataaatt cccactaggt aatttaccta
    32101 aaactaacgt taaatataca aaaatgttta ttggtgtaac tggctacgat actataatga
    32161 gcgtttatac agaagcaagc aagacaacta aaaaaaagta tatgatagag tctaatcttg
    32221 ataaatttaa tgttattgag aagggtgtag ttactttaaa tataacattt gaagaaggct
    32281 ttaatcttat caatacaaat ttttctgaaa gcattgagaa tgtaaaaaat aaagtactag
    32341 ttgttgatca gtatggtaat aaaattagtg agaaaataaa tgactctata tttaaagacg
    32401 ttggggtaat tatgcaaaag gtaatacaac aacaagaaaa cagtactata gacatagatg
    32461 gagagtttaa aggaatcgaa aaaacttgct ctttaaaagg atatggtgat ataacttgtg
    32521 taactggtag aggtgtaaaa gtaaaagact catatacaaa attaataggt cttttttata
    32581 tagatacaga taagcataca tggcaaaatg gagattatca aattgaacta gagcttaatt
    32641 ttgagaatat aatggatgaa aaatctgctg gccaagatga gcaaaaagaa gatggttcag
    32701 agtatactgg aggaacagaa taccctgctg aatttacggc ttattgtcct agaaaagaag
    32761 agggtggaaa tacagattgt agaggtaaaa aactagatcc ttctaaaaag acatgtgccg
    32821 ctcctatggt tggagcatat gaaaaatctt actatacaaa agatttctta aacaaacatc
    32881 ctcttatgag atatggagat gaaatacaat tagttacagg tgtttcaagc cgagatggaa
    32941 cgtataaagt aaatgataat gggcctgcaa taattataga aaaggatgga acatatcata
    33001 tagatatttt atttggaaat gttgaagaag ctaataactt tggaagaaga aaaggaaaga
    33061 ttataattgg tggttattct ggaaacatga cagaaaaagc aaaaacagta atttcagaag
    33121 caagaaaaca tcttggaaag ccatacaaat ggggtgggaa tggaccaagt agctttgatt
    33181 gttctggact aatggtatat tgctttaaga aggttaatgt taatttacca agaacctcaa
    33241 gtcagcaatc taaggtaggt aagaaagtag agaaaaataa tttacaggca ggagatttgg
    33301 tatttttcca taatccagtc agccatgttg gactatatgt aggtaatgga gaatttttac
    33361 atgcgccaca aacaggagat gtagttaaaa taagtaaatt aagtaataga aaagatttta
    33421 atacagctag aagagttttg taaaagaggt gatatcgtgg cagatccaat caatgaattt
    33481 ataggaataa ttaggcaaga aggtaaattt cataatgaac cttctttttt tattggaaaa
    33541 attaaaagta aattaccaga tttaaaaata gagataaata acatcatatt agaaaaagaa
    33601 gatattttga tagatagttg gatgcttgat agacagatag agttatttga tacagaaaca
    33661 agtcaagaac ataaacatga aataaaaaat ccctttatag atacttttga atctggaaac
    33721 acagtaataa tgtttaaaat aggtgaaaaa tttgctgttg taagtaaatt ggtgagtcta
    33781 gatgaataat actatattcc ctttcatggg tgttccagaa gattatattt ctccagacaa
    33841 tgaagaatta cctattttta aagaatttgc ttgggatttt aataaagatg aaaagataat
    33901 tgaaaatgga gactttaaaa tagttgaaag aaatgaagca attaaagtat ggatttacaa
    33961 aacaataaaa acagataagt atgttcattt aatatatgat tggagttatg gaacagatat
    34021 aaaaaatcta atagggcaaa agtatactaa aggtcttact gaaagtgagg caaaaaggta
    34081 tatacaagaa gctctattaa ctaatccata tatattagaa gtcaatataa caaatgcaga
    34141 atttgaagat gataatttat ctataagttt aaatgtaaaa acaatttatg gagaggagga
    34201 gattactttt gtataatggc caatcattca gtaccttaaa taatagaact ttaaataaca
    34261 taaatctccc tctttataaa ggccaaggat cttctcttta taatatagtt tctccaataa
    34321 attcagaact tgcacaatta tatatagaac tttcttatat acataagaga gtttttattc
    34381 aagataattt tgacgatttt cttgatagaa gagtcaatga atttggcgta tatagaaaat
    34441 taggtacaga ggcaataggt gaggtaacat ttgagggcaa aattggaact caaataccaa
    34501 atggaacaat aatatctcat agtgatttac tatttgtaat tattaaagat attacaatag
    34561 atgaaaatag taaattaaat gtaagcccta tacaggcttt agagattggc attaagtata
    34621 atttatcagc taacacagaa tttaaactta tagaagaaat aactggagta actaaaattt
    34681 ataatgaact tgacctaaag ggtggaacag aaatagaaac tgatgaagaa ttaaaagaaa
    34741 ggttctataa aatacaaaaa aatcaagcga caagtggaaa taaggcgcat taccaatctt
    34801 gggctttaga ggttgaaggt gtttataatg caaaagtaat tcctcgttgg gatggtccag
    34861 gtacaattaa aattctaatt tatggtcaaa acaatcaatc agttgacaat gaagtattgc
    34921 aaagatgtac tgaacatata gaagaagaaa aacctattgg acctactgtt acaattgtta
    34981 caccaagtac ttttgatatt aatattagtg caactttaac acttgaaaat ggatatgata
    35041 ttgaatctat aaaagttgtg tttttagata ttataaattc ttatttgata gaaaattcaa
    35101 gagagattgt ttatataaaa gtaatgagtt tgttagcaag catagaaggt attcatgata
    35161 taaaaaatct ccttgtaaat ggtgatgtta aaaacataat agttgatgaa gaaaaagttc
    35221 ctgctgtttc aagtgctata tttgatatcg aggtgagtta aattgaagtt aattgattac
    35281 ctgccaagtt ttgcaaacaa tgaaattgac atacaaatcc aagaagcact tgaaaatgaa
    35341 cttttaacgc taattgatga aaaagatgat ttattagaac agttttttat agatacagca
    35401 acttggggac ttgatgattg ggagaacctt ttaagcataa aagtaaatta taagttagac
    35461 ttcgatacta gaagaagcaa tataaaagca aagatgagag gtaagggtac aacaacaata
    35521 gaggttataa aagctatctc ggaagcttac acaaagacca atgttgatgt agaagtattt
    35581 agtaatctat ttagttttac acttagtttt ataacaaata attgtagtta taacactatt
    35641 ttagaattag ataagaaaat agaagaaata aaacctgcac accttgaaca caaattcgag
    35701 aggatattat ttaataaaaa cgagctttat acaggtgcag caattagtac aggagaaaca
    35761 gttacaatat atccttatgt acctagaaat ttagaaagtt ttggagaaat agctatttgt
    35821 agtggaaatg atagagcatt agaaaaagta acattgtatc ctaaaaaata gaaatgaggt
    35881 gataaaattg acagaacaac aatattttac tctagtaact gacattggta aggcagcaat
    35941 agcaaatgca agtattacag gtgaaaaagt agattttgca aagataaaag ttggagatgg
    36001 aggagggagt tcttatactc caaatgagag tcagacagca ctaaaaaatg tggtttggga
    36061 aagtacactt gaacatgcac aaggagataa agataatcct aactgggtag taatacaaaa
    36121 attcatacct ggtgatgttg gaggatttga aataagagaa gttggtctat ttgattctaa
    36181 agaccaatta ttagcgattt ctagttaccc aacaacatat aaacctactg cggattcagg
    36241 gactgtaaaa gaactattaa taaaagtaat attagttgta tctaatgtag ctaatattaa
    36301 tttgaaagta gacccaaccg taattttagc aactttaaaa gatatacaag aattagacac
    36361 taaaatagat acaactaaaa cagaattaac aagcaacata gaaactgcta aaacagagtt
    36421 gaacactaaa ataggagata caacacaact tactacaaca gataaaacaa atatcgttag
    36481 tgcattaaat gaggtaaaaa ctagtgtaga tagtatagaa acaacagcag ataaaacaag
    36541 tataaaagat acagataatt tatttgagag cgacaacgta gagggagcat taaaagaagt
    36601 gatgcaagaa gtaaaaggta atagaagcag tattatatca acaataaata gtaatttaat
    36661 accaatgtag aaagtgaggt gataaagtga agatatggaa atacaataga actgtgaaga
    36721 aaagaagagg tgtctataca gaattagata tacttgcaca aaggtatgaa tttgaagcag
    36781 atgggaaact ttatacagct tattttcatt tatatactaa taagaatata gatatattca
    36841 gagataaaga tttatataaa gaatcttata tgatgagcta tagagttgga aatgtaacaa
    36901 ctgatattga tgatgtagta cagcaaacaa ctacagtaag atttatacct gaccctgtta
    36961 ttgatattgt gattgataac attagcagag gatgtgaaat aaactacaga attatagata
    37021 atgaaccaag tgtaaaattc attgttacag aaaagttaaa tggcacaata atagaaacaa
    37081 agacaaattc tagtgataat aattataaac ttaaattaac tgatgaacaa atagtaccat
    37141 tagcattcaa ttcccaaaat tcaatagtaa tagaaataag tacagaagat ggtggattag
    37201 taacaagtaa aacagtaaca tttacaagaa caaataacaa gccaacaatt ttagttaatt
    37261 catatagttc taattctgca aaatttacag catctgattt agataataat ttaagtaaaa
    37321 ttgagtggta tttagatgat gtattaaaag aaacaataac aacagattta acagcagaaa
    37381 aaaccatcaa ctatgaattt acagacaatg caatacacac attaaaaata gttgctacag
    37441 atgcagaaaa tgcaacagtt gagaaggttt taagtataag taaagagata atgccacttc
    37501 aatctgatgc tagtttaagt gatatatcaa ctaagctgat agagattgga gaagggttta
    37561 gaaatggtaa aacaagtatt ataaacactt tagcacttaa aaatatagaa gcaagtttaa
    37621 ataatacact tgtggagtta tcagagaaaa taaaaacaag ttttgatagt tcagacgcta
    37681 gtgtgcagga tttgcaaaat agaataacag aattgaataa tcagcttagt cgaagaaaaa
    37741 aatgggctac aggtagatac acatttacta gagaagaaga tactatcaat ttcagaatgg
    37801 ttgatggaga tagtctttcc gcagtaaaaa aactaattat acccattaat ttagattttg
    37861 ttccaagtgt tattataata gacaaaatgt ttttaggtag ttacgtacct gcacaagatg
    37921 tgcaatttaa ttgtgtttca aacttagccg attttacact tggacgtcgt ttctataata
    37981 aacaatggat ttgcacttct tctataaaac tagattcagt tacacaaaat agttttactc
    38041 tttctttaca aaaagttgtt ccatttgata aagttagtag tacgattttt tctattatta
    38101 atactggttt tgcttggaga gcatatgaat aaaaatataa attagaggtg attaaatgaa
    38161 tagaaataat agaataattt acgaccaaac aggcaagata tggcttcaaa ctggtgaagc
    38221 aataggagat atacaagagt tgtcagaaat aactgaatta aatttcatag atgttgaatt
    38281 tggtagtata gactatagta aacaatatat agagtctata aatccagtta caaaagaatt
    38341 aaatataaaa aatatagaag ttgttttgac tgatgaacaa aagagattag aagtattaga
    38401 aaaagaacta aatatgttaa aagaagaaaa taaaaataga gattctgaga tagtaaatac
    38461 agcatttgaa gtaggaaata tcaaattaaa caataattta taggaggaaa ttatatgtat
    38521 aacttattaa agttaatgat agaacaaaaa aactatagca ctaaggagga tttacaacat
    38581 aagatggatg tattctatgc agtaaatagg attacagaag aacaatattt agagttaaca
    38641 ggtttattaa ataaagaaga aacaccagta gaaccaacaa tataggttct tttttattga
    38701 aagaaggtga ctaaatgact tttaaagaat tagttagtaa agttagaaat cttgtattag
    38761 aagcaaagaa tgtaactata gaagatacag agaataaatt cactagtgaa aatgtcgagg
    38821 gagcattgaa agagtgtata gatagagcag atttggcttt tcaaagtgcc gatagtggaa
    38881 aagagttaat tgcaactgct atcggctctc ctgctacatc cgaacaaaca tttcaagagt
    38941 atgctaatta tattgcagga tttaaaaata ctataagtca gttaaattct aaatataaat
    39001 atactagtgg aacttttacc tattatactg gtgcatatcc taatgggcta aatataggtt
    39061 ttagaccaga tattatttcc tatactgcca ttaatgctgt agaggaaaaa tattattgtg
    39121 tatacacacc tttattctat cttttttata attatacaac tggagaaaaa tatcttgatg
    39181 ttaatcataa tcgttatgga ataactttta ctgacaatgg atggatacct agaaatccag
    39241 ttaacaatgg tgtgtatagt tatatagcag ctaagtgccg ttagttgtca aagacttaga
    39301 taaatttcta agtcttattt taatacaaaa ttaggaggaa gtatgaacgt aacaatagtt
    39361 tttttagcaa caaatatatt tataaaatta gtaatattag caatagcatt tgatacattg
    39421 ctaggttgtc taagagcaat aaaaacacat cagtttaata gctcttttgg aataaatgga
    39481 ggaataagaa aagttgcaat gatagcatgt atattttttc tagcagtagt tgacatactt
    39541 acaaagttta actttttatt tatgttgcca caagattggg ttgatttctt acgactaaat
    39601 catctaggaa tatctgaatt tttctctatt ttattcattc tatatgaaag tgtaagtata
    39661 ttaaaaaata tgtatttatg tggattacca gttcctaaac gattaaaaga gaaaataggt
    39721 agtttattag atactatgac agatgaatta aatgttaaag gaggaaaata aataatgaaa
    39781 atatgtatta cagtaggaca cagtatttta aaaagtggag catgtacttc tgctgatgga
    39841 gtagttaacg agtatcaata caataaatct cttgcaccag tattagcaga tatatttaga
    39901 aaagaagggc ataaggtaga tgtaataata tgcccagaaa agcagtttaa aactaagagt
    39961 gaggaaaagt cttataaaat acctagagtt aatgctggag gatatgactt actcatagaa
    40021 ctacatttaa atgcaagtga taggcaagga aaaggctcag aggttctata ttacagtaat
    40081 aaaggtctag agtatgcaac tagaatatgt aagaaactag gtacagtatt taaaaataga
    40141 ggagctaaat tagataaaag tttatatatt ttaaacagtt ctaaacccac tgcaatacta
    40201 attgaaagtt tcttttgtga taacaaggag gattatgaga aggctaagaa acttggatat
    40261 gaaggtatgg ctaagttaat tgtagaaggt gtattaaata agagtattga aagtaatgag
    40321 gtgaagcaaa tgtataaaca cacaatagtg tacagtggtg atgatagagt atcagcagat
    40381 attttaggat tatattataa gagagcaaaa gaaagttatc tagttataga tataaaagac
    40441 tataaaccac atagaacaca aaatctatat gtaattggtg gagtaacttg taataaaatg
    40501 aaagaaatga gtaagactac aggagaaaaa tttactcaac tatatggtaa tgatgtatgg
    40561 tcaacaatgg ataaagttat agaatttgta aaagaaaaat tataaattaa aagacaacct
    40621 ttaaaaatta attgttaata aaactaataa aattttcaac tcagaaggag cattatatgt
    40681 taacattttt agatttgttt gctggcattg gaggttttag gctagcaatg gaaagagcag
    40741 gacacaaatg cgtaggccat tgcgaatacg ataaatttgc aaacttaagt tacaaagcaa
    40801 tgcataaccc aaaggaggat gaatggtttg aaaaagacat tagagaaatt agagcagaaa
    40861 atattcccaa agcagatgtg tggtgttttg gattcccatg tcaagacatt agcattgcag
    40921 ggaacaaact tggattcaaa ggaaatcgtt caagtttatt tttctcagtt acaaaactta
    40981 ttagagaact taaagaagaa gatagaccca agtacttact tattgagaac gttaaaaatc
    41041 tacttagtgt taatagagga tttgacttcg ccaaacttct cattgagttg gatgaaatcg
    41101 gctacaatgc agaatggcaa aatctcaatt ctaaaaactt cagagttccc caaaacagag
    41161 acagaatata cattgttgga catcttagaa gaagaagtac aagagaagta tttcctatca
    41221 caggaacgag tagaaaaact actcttgagc aattaaataa tcctaatcac agttcaaaca
    41281 ggatttataa ttcaaatgga ttatcaaagt gtttattaag taaaggtggg aacaatacag
    41341 gtctttatag agttggcaat atcaatccta gtggaaatgg tgcaggcgga caagtgtata
    41401 gtagcgaagg cttttctccc acacttacaa ctggaaaagg tgaaagccca aagatattaa
    41461 ttaaagagaa tactaaaaaa ggatacatca aagcatctat aggagatggg ataagactag
    41521 atcatataga aggtaaaact ggccgtggaa gagttcagca caaaaaaagt tgtactttga
    41581 ctactgcaag taatgctggc gtaattggta aaaaatttag agtaaggagg ttgactccaa
    41641 aagaatgttt taggttacaa ggatttccag acgattatta cgatagagcg gcaagtgtct
    41701 gttcagaaac tcaactatac aagcaagcag gcaatagtgt tactgtagat gttgttgaag
    41761 aaatagcaaa gaaattaaaa tagaatatta atacaatgca aaatgtggta ggtttttcct
    41821 tccgcatttt gcattgcatt agcttttttg gaataactta cctgtactat cttaaataaa
    41881 aaaatgctac tatattataa acatttacaa ggagtgcata atgttaatac aaagcttaaa
    41941 acgtggcgaa ttaattgtaa tgaaaatctt ttagaaaaga aattcaagaa tatcaaagaa
    42001 agaaattatt cgcatttgca atgaaaaaca caattggcgg aaatctgcga caaaaactct
    42061 acttaaaaga cttgttaaaa aaagagttct acttaagaga attaaaattt tccacatata
    42121 ttacatacct atagttacag aaaaagaata tttcaaatat aaaataaatg atttagagcc
    42181 agattattta gatgaatatt ttatacgatt aatgtccact gtacataaat ttaaaaatct
    42241 agatgaagaa gattaaatat ctcccttcat ctactcttca catattttaa tagactttca
    42301 agcaataatt gacttaacaa atcatgctta tcataatgtt tattctcatc tgcaaattta
    42361 ttaaacattt cccacactgt attatttatt ctaactgtag ttctttttat agattgatct
    42421 ggaagatcta tagatatatc attttcttta atgacacata tatgataact atcatcttta
    42481 gttttaaacc attccagcac attctttata ttatcaattt cactagataa atatttaata
    42541 tcgtcatcaa tcttactgct aatgctatct atatgatatt tatcatcata acttttatta
    42601 tcttctacaa acttgtttcc agttctttta tatcctttct tattaagcca cttccttata
    42661 gtactttcat ttgcccctaa ttcatctcca acttttttaa gactttcaaa ttcacttaat
    42721 ttattattaa tacaatcaac aacttcatta atattttttt ctttcaaatc actccaagtc
    42781 atataaatcc tcctcaaaaa aataatagat tacaaatatc atatatttgt aatctattat
    42841 acatactata aactaaatcc ttttttattt catcattttt ttgtatcctt ttgcatttgc
    42901 aaaaacatgt ttatctacat cactcataac tattatgcca ggtatattct ttttaaatgc
    42961 ttcacctaac aatttagcgc ctccacccat aacaacaacc gcagctgtac taactggata
    43021 atttaatttc aattcattgt atatttcttt aaatagagaa atagatgcat ctatattttt
    43081 ctttatatct tgtttttcac catttaggta aaatccttta tttatgtagt cttgtatttt
    43141 ttctcttttt atctcaactt tacctaaaaa tctttcgtcc atctcaagag atttctttat
    43201 tgcatcatat atatttattg ttcccacatt tatagaatat ggatcagtcg ctttattccc
    43261 ttgtacaagc gcaatatctg ttgtctttcc accaatatca actaaaacaa catctcttcc
    43321 tgcaatttgg tcaactatgt cttttgcata atataagtat cctgtaacac cttctggaaa
    43381 tgtttgaata gatcttattg ttatatctct tttagtagtc tcatttcctc ttgtaaactc
    43441 aacatgatat tcttttcctt gtaatttttc aataagattt tttcttaatc cggcaaattg
    43501 tttcactggc agacctagcc ctagatcaac aatttcatca tctgtatttc tacatatagc
    43561 ccctaatagt agtggtaaga agttttcttt ctcatattta actatattta tttcctcgtc
    43621 accttcacct aggtaatatg ttttattgcc tattttaatg ctatctgaat ctgaaccaaa
    43681 gttttttacc tctgtaactt tactttcaaa aatatcatct gtacttgttt taactgcata
    43741 atttccaata tctataccta atttactcat ttatgttatt tcccccttat tattctaaac
    43801 ttccaaaatc aaaattgtta gctggatctt ctatttcttt tttctgttca actacagcat
    43861 tatccttttt ttcttgcatt ttttcccaaa caagctcttt taagtaagct gtagctgata
    43921 atttctcatc taaaaacttt tcaattgctt tttctttatc attgttttta aatgttagaa
    43981 ctatcctact cattcaacca cttcc
//
LOCUS       DAMJVK010000026        43530 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.026, whole genome shotgun sequence.
ACCESSION   DAMJVK010000026 DAMJVK010000000
VERSION     DAMJVK010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 43530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43530
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.026"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(463..2529)
                     /locus_tag="PC414_001442"
     CDS             complement(463..2529)
                     /locus_tag="PC414_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDF2463841.1"
                     /translation="MVELIITTNIDIRKLNENQLEAVEHIDGPCMILAGPGSGKTRVI
                     TYRIANMVVNKNIAPTRILAISFTKASSIEMKNRALNLSDDIRLNKVTYGTFHSVFFK
                     ILRYFERYNLDSIFDEKSKRMTIKAILKSLNIENADDDENIGQVINEISYVKNELMDK
                     NEFNSEVLTKDEFLKVYNLYEEQKSKVNKIDFDDMLIRTYYLLLNNKSALEMVRNVYK
                     YILIDEFQDINKVQFEVLKLICSPLNNIFAVGDEDQSIYGFRGARPDFLLEFEQYFNN
                     TKKIILDINYRSKSEIVHTANRLIDKNKNRYEKIIKCSQGDGGSVTYISPHDSEEEAL
                     YIAREIIDEIQKDYVEYSDFAVIYRTNIQSRALVDVFMDMRIPFVVKDSVITIYDHWA
                     SQDILAYLRIGINPKSNKDWLRIINKPFRYISKDSINMVKDEKDFITALINKCNLHPK
                     QVKTINDLEIDLSYLNTLNPKNAISYIRTSLDYDRYVLDYCSNRKIKTNGLVEILNEL
                     ESSATNFNTVTEFLEHIDRVKTELTENNKNKQTEGVIFTTMHSAKGLEFRNVYIIGVN
                     EGTIPHEKSYDICKEEKKEEQLEEERRLMYVAITRAEEKLCISSTLNKYGKKVDKSLF
                     INDIKSPTKKEIDSIGVGDKVYHKKFHEGEIIKKDGIMFTIKFKDRERILDLKTCLLK
                     NIIYTI"
     gene            complement(2606..5635)
                     /locus_tag="PC414_001443"
     CDS             complement(2606..5635)
                     /locus_tag="PC414_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2463842.1"
                     /translation="MENNLRKNNSIMKKMIFPLIIVMLIQTSLFCATILWGGTIKKLN
                     DNSFDILNERVINRKNYIQNEMLQRWSNTSETEATINSSVKKVLNEKNALIEDVGNNE
                     EINKDIIKSISNDLIYLLRKNSVTGAFIILSDKNINDKSIDSKGEISRTGLYIRDLDP
                     KFNPNDNSDLLIERGSSDIARSFGISMDSYWEPKFSFKSENEQDGDDFFYKPFRAAID
                     NPNVSSSNLGYWGSKFLLTNAGDRLMTYTVPLIYEDGTVYGVLGIDLSVNYLRELLPY
                     SEINGNKKGNYILGVDRNNDMAFENVISNGYSLKEKFWDGLHSNLEQKESYKNIYKIK
                     NKDNECGNGNIYASVQYLDLYNSNTPFENERWALLGIIEEKSLLSFSKRVEKLVIISI
                     AISLLIGLIGIYIASRLFTKPIVLLANKVRNSNPSKPVNLDKINISEIDELSSAIELL
                     SANVADSSSKLSQIIEMVDMPIGAFELYKNAENVFCTKEFFSVFTTEIKFEEYGYMEK
                     NKFLKKLRGLDKYLQESYETENTYIYKFESKDKKLKWIRMNIFEDELKVLGVVVDVTK
                     EFIEKRKIEYERDFDILTNLLNRRAFHSQIQEKFKNKDELKIAAFIMWDLDNLKYIND
                     TYGHDAGDEYIRCAADILKKFISWNGIVSRLSGDEFYVFIYGYESKDSIRDIINSVKK
                     QMNNTLLKLHDGTEFKIRASAGISWYPDDSTNYDELMRYADFAMYQIKNTIKGEVSEF
                     DINIYNKDSFLLSNREELNKLIDGQLVEYAFQPIVDCKGKVFAYEALMRPKIKTFSSP
                     IDVIRLARSQSKLYQIERITWFKALKSFKKYKEKFGDCKLFLNSIPNNNLSKVDFEEI
                     EDLYGEYLNRVVLEITENDKINEDFIEKKKAYIDHWNIEIALDDFGTGYNGDAVLLYI
                     MPDYVKIDMSIVRGIDKDENRQKILKNLISYSNERNIKVIAEGVETKGEMETLINLGV
                     DYMQGYYISKPSFIPEDISDKIKKELKICHENRNDVV"
     gene            complement(5688..7148)
                     /locus_tag="PC414_001444"
     CDS             complement(5688..7148)
                     /locus_tag="PC414_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDF2463843.1"
                     /translation="MNRCKFAKLIITSMIVLLLTGCSLKKEDNYKLDPENPTSIQIWH
                     YYNGQQKMAFDKLVKEFNDTVGAEKGIIVEAFSQGNVVDLSEKVLDSANKKVGSEDVP
                     NIFAAYSDTAYEMDKLGLLVDMDKYISKEELNEYIDEYVEEGRINSKDEFKLFPIAKS
                     TEILMLNKTDWDKFAKATNSNINDIKTIEGLVETSKKYYEWTDSLTKEPNDGKAFFGR
                     DALANYIIIGSKQLGVEIFSVKDGKVTLNIDEKVMKKLWDNFYVPYINGYFASYGKFR
                     SDDAKTGDILALVGSSSGAAYFPDRVMLNDNESYNIETSVFQAPRFKGEGKYAVQQGA
                     GMAVTKSTKEEEYASVEFLKWFTDKEPNIKFSIASGYLPVKKKTNDLDVIKEVMKKDN
                     LSLSDKVEGSIASSIEQINSCNLYTNKAFDGGTKARQILEYSMLDKAKEDSNKINELV
                     KSGTSKKDAVAQFNNDKNFKTWFESFKAELENAIKK"
     gene            complement(7240..7614)
                     /locus_tag="PC414_001445"
     CDS             complement(7240..7614)
                     /locus_tag="PC414_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463844.1"
                     /translation="MGNKNKNTKYKDKTRERYNKKVENLNKEKLNKINKQSKKVDKID
                     DIINKSSIVLCVLLVVFSLLYLFKLVQLNIIMILLVITVAIQQGLVSFGAFRNKNYVK
                     ALFSIFVIVCFLIAFKYVVNPV"
     gene            complement(7790..8530)
                     /locus_tag="PC414_001446"
     CDS             complement(7790..8530)
                     /locus_tag="PC414_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDF2463845.1"
                     /translation="MLYDEDEKEENNERWLLTYSDLITLLMIFFVIMYSMSNVDAEKY
                     KQLSQSLNSAFGGSSGVIEGGNSKIEPVVEPGSNDLDTLQNAKFKKIGEEIQKYLNEN
                     GMANSVSLRVQDRGLVISLKDTILFDTGKAIVKDNSRDKIIQIGKMLNEMNSYMRVEG
                     HTDNMSIKNSEFKSNWDLSVMRATNVVQLLIDNAGIAPDKLSAVGYGEFRPIAENSSE
                     EGRSKNRRVDIVLVDSKYDNVENVSKDK"
     gene            complement(8551..9339)
                     /locus_tag="PC414_001447"
     CDS             complement(8551..9339)
                     /locus_tag="PC414_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HDF2463846.1"
                     /translation="MTTLIFFIVGIASLVVAFVIDGGHVGALFVFSSALVVFGGTIGA
                     VGASTPFPVFKKSIKIMLLAFKNKKPNNIENIIYFKEIATKARKEGLLSIDKEISEND
                     ELDPFIRKGLELMVDGVDAVTIRNILESQIQITSKRHKEGIAIFDSAGGYAPTLGIIG
                     TVMGLVHILGNLSSNPDALGPQISVAFLATLYGVGSANLIWLPIASKLKAINQQEVSE
                     RLLVIEAILSILEGDNSNLMTEKLKIFLNSKELIELEEIGGRAE"
     gene            complement(9427..9517)
                     /locus_tag="PC414_001448"
     tRNA            complement(9427..9517)
                     /locus_tag="PC414_001448"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9481..9483),aa:Ser,seq:gct)
     gene            9776..10540
                     /locus_tag="PC414_001449"
     CDS             9776..10540
                     /locus_tag="PC414_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDF2463847.1"
                     /translation="MLPQERYEKILSILKSDNIIKINDIVQMFDISIETARRDLAHME
                     SIGLLKRVYGGAIPVIASSVELNYVKRKKINSNDKKQIAIKCSEFINTNDTIFMDTGT
                     TVLEVSKNLKDKKNLTVITNSILVANELMNTNIKVYVLGGYLRETEGSFSGPLTLSAI
                     EQFNVDKAILGAGGITLENGISDYHLEEALVRKKMIERSRKSIVVVEPPKFGINSFAS
                     VAPIEHIDTIITSASIDHNIIIEFSQKGIPLVIADN"
     gene            complement(10821..11936)
                     /locus_tag="PC414_001450"
     CDS             complement(10821..11936)
                     /locus_tag="PC414_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HDF2463848.1"
                     /translation="MPFDKIILYIMAVGVLLGALDRIIGNKFGLGEQFEEGFNSMGPL
                     ALGMVGIVCLAPVISDVLGPIIIPIFNLCGADPSMFATILANDMGGYPLAMELAHNKE
                     AGMLAGLVVSSMLGCTLVFSIPVGLGLIEYEDRPFFAKGLLIGLITIPFGGIIGGLIA
                     GFNLSMVIINMIPVIILAVILAVGLKFNSTLMINGSLIFGKFISILITIGLGAAAFQE
                     ITGIVLIKGMTPIREGIQVIGSIAIVLLGTFPILHLLVKVLNKPLTIVGGKLGMDATS
                     AAGLVFTLANSIPVYKMMKDMSPKGKVVNTAWLVCATAALGDHLGFTAGVVPEMITPV
                     VIGKLVGGVLAIALAILMTKDLTLEEEQSIKMKKDAV"
     gene            complement(11977..13503)
                     /gene="glpK"
                     /locus_tag="PC414_001451"
     CDS             complement(11977..13503)
                     /gene="glpK"
                     /locus_tag="PC414_001451"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDF2463849.1"
                     /translation="MKYVLAIDQGTTGTRAVLINEKSEFVFSDYMEHTQIYPQSGYVE
                     HDPIEIWNNTKTVSNNVLQQAFENGINESDIKGIGIDNQGETVMLWDKNTGKPLYNAI
                     VWQCRRTYDYVENLKNIPGLENKIIDKTGLIIDPYFSGTKIKWVIDNVKGVKEKIKNG
                     EVLAGTLDSWLIWKMTGGKSFLTDVSTAARTMLFNIRDMDWDRELLDIIGVPRSILAD
                     IMPTSGDFGTTDKEAFCGVSIKITASMVDQPAALFGNGCFEPGMVKNTYGTGCFLYMN
                     IGEELNIAGNGILTTVAWKIGDKVTYAYDGGVYIAGAAIQWLRDGIGVIKNYSETDDM
                     ANSISSTGGVYFVPAFAGIAAPYWDQYARGTMVGITGSTKKEHIVRATLESVALQVKD
                     VVDSMNLASGKKIKMLRVDGGITKNNFTMQFQSDILGIPIEIAENPETTVLGVGYMAG
                     LSCGVWNSIDEIRSEFTISKKYIPNMTTQKREEILLGWNQAVKRAMNWENDTKKVCAN
                     "
     gene            13796..14893
                     /locus_tag="PC414_001452"
     CDS             13796..14893
                     /locus_tag="PC414_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HDF2463850.1"
                     /translation="MKKTYLFSPGPVMVTDKVRNSLLHYDICHRGNEFMDLFKDTQQK
                     INKLYNATSNYYSLIVSGSGTSVNECVLSSIFDKEDAVLLVSNGVFGERLEEILSTYN
                     VKTYKPNFEWAEYPDLDILEKYIIENSDIKVIAMVFHETSSGMINPVPELGKLAKKYN
                     KIFFVDAISASAGEYIDVEEFNIDIITGVGGKALGAFPGSAYLCAKESILQNIKENQC
                     KNVYLNLYKHYKVAKSSSQTPNTPNVTLIFALNEALTEFLEDDSKIERYKECSAILRY
                     GMEELGLTFLLPDKYMSNTVTSVFLPKEINVNEFILGLERESGYVVYPGKGKFLDANI
                     FQVANMGEIYPEDCYKFLDILKSKLEIKKVI"
     gene            complement(15384..15896)
                     /locus_tag="PC414_001453"
     CDS             complement(15384..15896)
                     /locus_tag="PC414_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463851.1"
                     /translation="MKVKKFKSGIVMALTVMAIGASLTNVSAMELNKSKEFETKYNEM
                     KRDFQEEYGKEDKFNITLDDEFTKEEVNGAKGIIKTTNKMNNEVVVLNYFDEMKSGIE
                     FELKPKAEKNAILRDEFQQGYGKEDKFKVVLDDEFTKEEVNGAKGIIKTTDKETGEVI
                     VYNYFDELEK"
     gene            16449..17330
                     /locus_tag="PC414_001454"
     CDS             16449..17330
                     /locus_tag="PC414_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HDF2463852.1"
                     /translation="MKKRLIIMILSVVLVLSSILTIFAYSNIKYNNNKLIYSNMIDKK
                     TQNSVKEILKENKINEKDIDTFIKSVNNYNKLQVKILQNNINISKSGYSSINAKQVPY
                     NLEKLQDNWVKKFPDYMDVNCRITAFRLFKDFINSNKKFTGDSIDLNVDLDTIMNNKD
                     AKFSTKDVEKFINFFSAIPAKDTDDTIKIAEQIKNEWKKRKISFKNNKNISIINGFLR
                     YPETKNVFIGHTGICIKTKNGILFLEKYGVTSPYQVTKFKNKEDVKNYMFNRLKMSEG
                     EIELPDPIIMENDKLMK"
     gene            complement(17643..18422)
                     /gene="hisJ"
                     /locus_tag="PC414_001455"
     CDS             complement(17643..18422)
                     /gene="hisJ"
                     /locus_tag="PC414_001455"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ"
                     /protein_id="HDF2463853.1"
                     /translation="MKDGHIHSPYCPHGSKDDFEKYVQRAMDVGITEMTFTEHFPYAN
                     GFRDPAPENDSCMSIEDLPKYFNDVKKLKEKYEGKIKINVGSEVDFIEGYEEGIKENL
                     NKYGKELEDSLLSVHIIKIDDDYYCVDYSVEEFQNLIDKLGSIEAVYNKYYETLIKAV
                     NSDLGIHKPKRIGHLNLVRKFNQVFPYDYKGNKILEELIKLIKEKGYELDYNVSGNRK
                     EYCKEPYIDGYLLDLVKKYDIPLVLGSDSHCAEEINKYDII"
     gene            complement(18968..19318)
                     /locus_tag="PC414_001456"
     CDS             complement(18968..19318)
                     /locus_tag="PC414_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter"
                     /protein_id="HDF2463854.1"
                     /translation="MIFDKEIVRYHHGHHDHDHCHEHTHGDICHEHPHDHAHDHDHEH
                     SHEESSESKDEKTLKILLVHWINHNETHEEGFREWVEKARAIGKEETAKSIEKAIEYM
                     EEANKMLLEAKKHM"
     gene            complement(19334..19750)
                     /locus_tag="PC414_001457"
     CDS             complement(19334..19750)
                     /locus_tag="PC414_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HDF2463855.1"
                     /translation="MIIAVIDGMGGGIGAQIVSSLREELPTYVEIYALGTNSIATSSM
                     MKAHANKGATGENAIVVSAKKANIIVAPISVIIPNSMMGEVTCNISEAIADSEALKIL
                     LPIMPENVELVGLEGKPLALLVKDSVNLIKKEFNIK"
     gene            complement(19769..19960)
                     /locus_tag="PC414_001458"
     CDS             complement(19769..19960)
                     /locus_tag="PC414_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CooT family nickel-binding protein"
                     /protein_id="HDF2463856.1"
                     /translation="MCESSAFICKSNNELEKVMENVVNIDPCDGKIYLTDLLGEQKII
                     DGIIKEIRLMDHKIIIQEN"
     gene            complement(20111..20926)
                     /locus_tag="PC414_001459"
     CDS             complement(20111..20926)
                     /locus_tag="PC414_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HDF2463857.1"
                     /translation="MNQWRVIHNKSYEGAMNMAIDEAIFTAYKKGHNKPTLRFYTWEP
                     ACLSIGYFQKLEDEIDLDKCRCMNIDYTRRITGGRAVLHDNELTYSIIIGEDNPLIDK
                     SINLSYRYISEGLVKGLNLSGIETDNLNRGERISRENLSAACFNAHASYEVTINNKKV
                     IGSAQSRKDGVLLQHGSIILDFDVEKLFKLIKTKTPELKERAMKFTAKKASGIENEIG
                     RKIDIDILQKNIVKGLAEQFNVEFVEGDLTDYEKQLVKELYEKYKNEEYNKKR"
     gene            complement(20973..21911)
                     /locus_tag="PC414_001460"
     CDS             complement(20973..21911)
                     /locus_tag="PC414_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2463858.1"
                     /translation="MIRLSVGTAIELGILNKKSDIPPTTAYIMIGEKCINKCSFCSQS
                     IESSTRKDKLSRVIWPEFSKEEILDALKAYKGKNIKRICIQSMASEEAHNSVLDFINY
                     ISGKIDMPISLSAKLENDEQIKKFFSAGVDKIGIAIDAANKELYEKIKGNNYDEKLKF
                     ITEMSKSYPNKISTHIIVGMGESHEDIYNLYTYLKENDVTISLFAFTPVRGTKMEKIN
                     QPSIESYRRVQLMSYMINKGYPKEYFKFKNGYLNSIKLDNDILKDINKGYPFEIRGCK
                     DCNRPYYNERPGSTIYNYSRPLNQSEIDLAIREINL"
     gene            complement(21908..22780)
                     /locus_tag="PC414_001461"
     CDS             complement(21908..22780)
                     /locus_tag="PC414_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2463859.1"
                     /translation="MNKNLSEKMKIAFDIKLKNFGNKIEFAYPNQTLALSTTSNQCSL
                     KCAHCNGHYLNNMVPIEEYEEKVQSRNITSFLLSGGCSYEGDVPINTHINTIKNLKEQ
                     GYRLNAHLGLMDKDSIVELCKYLDIVSFDLVFDDETIREVYKMKKSKEDYIEVYNTIQ
                     EHTEVAPHICIGLKGGQIKGEYEIIEYLQKNPPNKLTFIVLIPTKGTEYENVEPPELE
                     GVADILCEARINLPDTEINLGCMRPRGVYRKELDQLSIMCGVNRIVLPSRSAKNKAIE
                     MNMTINECKECCVL"
     gene            complement(22805..24733)
                     /locus_tag="PC414_001462"
     CDS             complement(22805..24733)
                     /locus_tag="PC414_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4445 domain-containing protein"
                     /protein_id="HDF2463860.1"
                     /translation="MIKVSFTPNNKEVYCNEGDILLEVARNADIFIDAPCNGNVSCGK
                     CKVKLLNGKVDTEKTRHITDDEWEQGYILACCTKVISDIEIEVPSKVSSSMHGMKIEG
                     SNKKEDREIFERAKKIIEEHNLQFKTNIKKKYIEMEEPNLDDNISDVDRLERYVRNNL
                     GYNEIDFRLDILRKMPTVFRKSDFKVTITYVQKQKKLTIINIEQGNKENSLYGVAIDI
                     GTTSVVVCLVDLYSKEVVDKASSGNAQIKYGADVINRIIYSTKKNGLETLHKAIVEET
                     INPLLKTIYERNGIDKEDVVTLVAAGNTTMTSLFLGVYTDFLRQEPYIPPFLKSPKLM
                     GENVGLFVNDSAYVYLAPSVASYVGGDITAGVLSAGIWSSEENVLFIDLGTNGEIVFG
                     NKDYMMSCACSAGPAFEGGGISCGMRASAGAIEKVIIDKDTLEPTLKIIDECAPVGIC
                     GSGIIDLICQMITKGVIDRRGKIYRDLDNKRVRFNEHEIGEYVLAFKEEFDLENDIVV
                     NEVDIDNFIRAKGAIYSGAYTLVDSLGMDFSILDRVYIAGGIGNNLDIENSIIIGLLP
                     DIDREKFTYIGNSSLVGSYLALISKDAKNKLEEIGNQITYVELSVYPSYMDEFISACF
                     LPHTNIEQFPTAKKLLEE"
     gene            complement(24845..25222)
                     /gene="gcvH"
                     /locus_tag="PC414_001463"
     CDS             complement(24845..25222)
                     /gene="gcvH"
                     /locus_tag="PC414_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HDF2463861.1"
                     /translation="MKLLPELKYSKDHEWVKVIDGDVVYIGITDYAQDQLGEILFVET
                     PEVEDTVTKGVDFGVVESSKVASDLISPVNGEVLEVNEKLEDEPECINEDPYENWILK
                     VKLADVAELDTLLSDKEYEAGLE"
     gene            complement(25309..27435)
                     /gene="cdhC"
                     /locus_tag="PC414_001464"
     CDS             complement(25309..27435)
                     /gene="cdhC"
                     /locus_tag="PC414_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CO dehydrogenase/CO-methylating acetyl-CoA
                     synthase complex subunit beta"
                     /protein_id="HDF2463862.1"
                     /translation="MNLYNIIFTGSEQALGAAQAMLAEAIEKNGKEHKVAFPDTAYSL
                     PCIYAATGQKMNTLGDLEGALEVVKSLINRTHLLEHAFNAGLATALAAEVIEALKYST
                     MDAPYSEPCAGHITDPIIRSLGVPLVTGDIPGVAVVLGECPDAESAAKVIKDYQSKGL
                     LTFLVGKVIDQAIEAGVKMGLELRVIPLGYDVTSVIHVVSVAVRAALIFGGLTPGDLN
                     GLLEYTANRVPAFVNAFGPLSELVVSAGAGAIALGFPVITDQTVLEVPMNLLTQKDYD
                     KIVATSLEARGIKIKVTEIPIPVSFAAAFEGERIRKSDMFAEFGGNRTEAWELVVKKE
                     ATEVEDHKIEIIGPNIDEVDADGVLRLPLAVIVKIAGKNMQEDFEPVLERRFHYFLNY
                     IEGVMHVGQRDMAWVRISKDAFDKGFRLEHIGEVLYAKMLDEFESVVDKCEITIITDA
                     EKVSELKGEAIAKYNARDERLASLVDESVDTFYSCNLCQSFAPAHVCVVTPERLGLCG
                     AVSWLDAKATKELDPTGPCQPIEKGECLDDRTGVWNSVNETVNQISQGAVESVTLYSI
                     LEDPMTSCGCFECICGIMPEANGFVVVNREFASVTPVGMTFGELASMTGGGVQTPGFM
                     GHGRHFISSKKFAYAEGGPERIVWMPKELKDYVADKLNATVKEMTGIENFCDMVCDET
                     IADDSEGVLAFLEEKGHPALAMESVM"
     gene            complement(27492..28298)
                     /locus_tag="PC414_001465"
     CDS             complement(27492..28298)
                     /locus_tag="PC414_001465"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HDF2463863.1"
                     /translation="MEKFMIIGERIHCISPSIRKALAERDPAPILKRAKEQLEAGAHY
                     IDFNIGPAERDGEEIMTWGIKLLQSEFNNVPIALDTANKKAIEAGLKVYDRTNAKPII
                     NSADAGSRFDLIDIAAEYEAMVIGLCAKEGIPRDNDERMAYCQEILEKGLMLGMEPTD
                     ILFDPLCLVIKGMQEKQVEVLEAIKMMTEMGLLTTGGLSNVSNGCPKHVRPVLDSAFL
                     AMAMANGFSSAIMNPCDPELMKTVKSCDIINGASLYADSFLELNEGGFAF"
     gene            complement(28348..29715)
                     /locus_tag="PC414_001466"
     CDS             complement(28348..29715)
                     /locus_tag="PC414_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HDF2463864.1"
                     /translation="MALKALDIFKLTPKKNCKDCGFPTCMAFSMKVASGAVEVGKCPH
                     MSDDAIAKLSEATAPLMKALKVGAGASEYELGGETVLFRHEKTLVSRNRYAVSFCTCM
                     SDEAVDAKIANMKKVDYVRIGEQMKVEMAVLEYCGDKDAYLKLIDKIKGSGLEVAYIL
                     ACDDAQVVKEAVEVLKDARPMVYGATKENYKDMIEVVKGASLPLGVKAGSLEELYETV
                     ELIQAAGYKELVLDVTGENIKDTYTNAIQVRRTALKEQDRTFGYPSIVFANRLSNSNP
                     MMEVALSSIFTIKYGSIIVIDDISYAKALPLFALRQNIYTDPQRPMRVEPKIYPINNP
                     DENSPVLVTVDFALTYFIVAGDIERSKVPVWLVIPDAGGYSVLTSWAAGKFGGNSISA
                     FIKESKVEEVTNCKDLIIPGKVAVLKGDIEDNLPGWNVVIGPEESMELPKFLKGYQEK
                     ACQTN"
     gene            complement(29746..30690)
                     /locus_tag="PC414_001467"
     CDS             complement(29746..30690)
                     /locus_tag="PC414_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit delta"
                     /protein_id="HDF2463865.1"
                     /translation="MAFKMSTQKYSGKISEVEVGIGEKAIKLGGENVLPFYSFDGEVG
                     NSPKIGIQISDVYPESWTDSYKELYKDVANCPVEWAKYVEANTQADFICLKFDGSDPN
                     GLDKSVDECADVAKAVIEAIKLPLVVAGSGNHEKDGKLFEKLAQTLDGHNCLFMSAVE
                     DNYKGVGASAGMAYAHKVGAESSVDINLAKQLNVLLTQLGVKGENIVMNVGCSAVGYG
                     YEYVASTMDRIRLAAFGQNDKTLQMPIITPVAFEVGHVKEAIAPIEDEPDWGCPEERT
                     IAMEVSTAASVLVGGSNAVILRHPKSIETIKELVNALA"
     gene            complement(30710..31480)
                     /locus_tag="PC414_001468"
     CDS             complement(30710..31480)
                     /locus_tag="PC414_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2463866.1"
                     /translation="MGYNIAVAGKGGTGKTSLTGLLIDYLVKDKKGPVLVVDADANAN
                     INEVLGIEVEATIGEIREEVNQREKLGNAFPGGMTKAQYLQFRLNSIIEEGEGYDLLV
                     MGRSEGEGCYCFVNGILREQVNKISGHYKYLVMDNEAGMEHLSRKVTRHVDTLLLVSD
                     CSRRSIQAVARIRDLAEELKLSVGRILLIVNKVPNGVMNDGVKEEIEKHNLELIGVVP
                     MDELIYEYDSTGIPLVNLPEDSKSKVAMKEIFAKLELK"
     gene            complement(31536..32921)
                     /gene="lpdA"
                     /locus_tag="PC414_001469"
     CDS             complement(31536..32921)
                     /gene="lpdA"
                     /locus_tag="PC414_001469"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDF2463867.1"
                     /translation="MKIVVVGGGPGGYVAAIKASMLGADVTVVEKRRVGGTCLNAGCI
                     PTKALLASSGVLNTVKEAKDFGIEIDGTIKPNFTAIMERKNKVVNQLISGIEFLFEKR
                     GVNLVNGFGKLIDKNTIEVTKDDGTVETIKADKIILANGSVPVVPRMFPYDGKVVITS
                     DEVLGLEEIPESMLIVGGGVIGCEIGQFFRALGTEVTIVEMVDQILLNEDKDVAKQLL
                     RQFKKDKIKVITGIGVQTCEVVDGKAVATLSNGKVIEAQYALVCVGRRPNLDNSGVED
                     IGIEMERGKVVVNEHLETNVEGIYAIGDIIDTPFLAHVASKEGIVAVENALGKTKVVD
                     YRAIPRCVYTEPEVAGVGKTEKQLEAEGVEYNVGQFDFRGLGKAQAIGHFQGFVKVIA
                     DKETDKIIGAAVVGPHATDLLTELSLAVHLGLTVEQVGDAIHPHPSLSEGLMEALHDV
                     HGECVHSVPKL"
     gene            complement(32965..33846)
                     /locus_tag="PC414_001470"
     CDS             complement(32965..33846)
                     /locus_tag="PC414_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HDF2463868.1"
                     /translation="MSLLRETLESGKFAVTTEMAPPKGTDLSHLIECAKPLVGRVHAA
                     NVTDFQSAVMRATSLATCKLLKDAGLEPVIQITGRDRNRIAIQGEMLSAGVFGINNLL
                     ALTGDHTSVGDHPQAKGVFDLDSVGILQTAETLMAGTDMAGNKLKGSPDFYLGASVTP
                     EYSPIEVQLLKMKKKIKAGAKFFQTQALYDINTMRKFRELTRDMDCKVLAGIVPLKSP
                     GMAKFMTANVPGIFVPDEQIERLRAAGKENWVSEGIKMAGELIKQLKEEDLCDGVHIM
                     AIGAEENIPAILDAAGL"
     gene            complement(33881..34555)
                     /locus_tag="PC414_001471"
     CDS             complement(33881..34555)
                     /locus_tag="PC414_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HDF2463869.1"
                     /translation="MIISENKPLEEVLGYLKDFDKLVLVGCNQCAATCKSGGEEEVLK
                     MKETLEGEGKKILGYVMLDPACNLLKSKKDLKALKEETKEADAVLSLACGDGTQTIVK
                     NLKDKPVYPANNTLFIGEVQRVGEYEEACKACGDCELGWTGGICPVTMCAKGLMNGAC
                     GGAKNGKCEVNSENDCAWIKIYERLEAIGQLDNLAEIRPPKDYSKQNNPRSLSAKKKK
                     EAAANS"
     gene            complement(34592..35464)
                     /locus_tag="PC414_001472"
     CDS             complement(34592..35464)
                     /locus_tag="PC414_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylene-tetrahydrofolate
                     dehydrogenase/5,10-methylene-tetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HDF2463870.1"
                     /translation="MEGMSTKGQIIKGKPVADKISEELIKEVDLLIKEGINPKLTIVR
                     VGARSDDLSYERGALKRCQNIGITTEVLELAEDITQEEYIDVLKRVNDDKNVNGILCF
                     RPLPKHLNEEVIKYVIAPEKDVDCFSPINSAKVMEGDKSGFPPCTPTAVVEILKHYNV
                     DLKGSKVTVLGRSMVVGKPVSMLLLSEHATVTICHSKTKNLSGVAAEADVLIAAIGRA
                     KMVDESFVKDGAVVIDVGINVDEEGNLCGDVDTNAVLDKVSMITPVPAGVGSVTTSIL
                     AKHVVKACKLQNNK"
     gene            complement(35556..36188)
                     /locus_tag="PC414_001473"
     CDS             complement(35556..36188)
                     /locus_tag="PC414_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HDF2463871.1"
                     /translation="MKIADKTCVDFVEVLSSKEAVPGGGGAAALVGAIGMALGSMVCN
                     LTIGKKKYAEYEESVKEILSKAGKLEKDLLKMIDDDAECFLPLSKAYGLPKETEEEKR
                     IKAETMEKALKVACEVPLNIVRVCYEAIKLHEDLVDKGSRLAISDVGVGVQCLRAAIL
                     GGQLNVVININSIQDKEYVNKVKTEVDKLVEDGVKICDEVYAKVEKALGK"
     gene            complement(36293..37969)
                     /locus_tag="PC414_001474"
     CDS             complement(36293..37969)
                     /locus_tag="PC414_001474"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HDF2463872.1"
                     /translation="MGFKSDIEIAQEAKPQDIREVAKKLGLGEDDVELYGKYKAKVDY
                     NLLKRETGKKAKLILTTAINPTPAGEGKTTTTIGVADAFAKLDKNVLVALREPSLGPV
                     FGVKGGAAGGGYAQVVPMEDINLHFTGDFHAIGAANNLLAAMLDNHIHQGNALRIDPK
                     KITWRRCVDMNDRQLRNIVDGMGKKGDGAVRQDGFDITVASEIMAAFCLASDISDLKE
                     RLGNIIVGYSYEGEPVTARQLKANGAMAALLKDALKPNLVQTLEGTPSFVHGGPFANI
                     AHGCNSVIATRMAMHFADYVITEAGFGADLGAEKFLDIKCRMANLKPDAVIIVATVRA
                     LKYNGGVAKADLNNENLEALEKGLPNLLKHVENITQVYGLPAVVAINRFPLDTEAELK
                     LVEDKCKELGVNVALSEVWAKGGEGGIAVAKEVLRLLDEEENNFRFCYEDDLSIKDKI
                     NAIATKIYGADGVDYTPEADKEIANLEKLGFTKVPVCMAKTQYSLTDDQTKLGRPTGF
                     RITVRQATISAGAGFIVALTGEIMKMPGLPKVPAAEKIDVDENGVIAGLF"
     gene            complement(38218..38997)
                     /locus_tag="PC414_001475"
     CDS             complement(38218..38997)
                     /locus_tag="PC414_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2463873.1"
                     /translation="MKIAITGKGGVGKTTFSSMLSRMFAEDGYRVVAVDADPDANLAL
                     ALGFPKEVYESIVPISEMKKLVSDRTAASVGSFGKMFKMNPKVDDIPENFCKEYNGVR
                     LLTLGTVDSGGTGCVCPEHVLLKRLCSHLILQNKDVVVMDMEAGIEHLGRGTAQGVDA
                     FIVVVEPGERSLQTYRKVKKLGHDIGVNKVFVVGNKIRNKEDEEFIIQNLEDGESLGF
                     IYYNQDVIDSDRANQSPYDSSETTKEQIKAIKDKLMSLKDK"
     gene            complement(39307..41226)
                     /gene="cooS"
                     /locus_tag="PC414_001476"
     CDS             complement(39307..41226)
                     /gene="cooS"
                     /locus_tag="PC414_001476"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HDF2463874.1"
                     /translation="MDEKMLTIDLNSQKMIAKAREEGVETMYDRKEGFKAQCGFGLQG
                     VCCRICGMGPCRISPKTPRGLCGADEHTIVGRNFARMVAGGTAAHSDHARDIAHTLAL
                     ADPNGNYKIRDEAKLITLAKEWDVETEGRDIYDVAHEVAEIALMEFGKPFGTLRFIKN
                     APEPRQKIWKEYAIEPRAIDREIATIMHSTHIGCTGDIDSLIHMSLRTSMADGWGGSM
                     IGTRLSDILFGTPVPRRTEANLAVLEENKVNIILHGHEPALSEMIVLASEEPDLVALA
                     KEVGADGINLAGMCCTGNEITMRHGVKIAGDFHQQELAIITGAVEAVIVDVQCIFPAL
                     ARVADCYHTKFVTTSPKAKITGSTYIEFREEQALDDAKAIVKEAILNFKNRDKSKVLI
                     PELKSGATVGYSVEAVVNQLDRVVNSHIDPAGTVKPLTDCLKSGVLRGAAGVVGCNNA
                     KGVSNEAHVTIMKELIKNDIIVVTTGCGASAAAKFGLMESDAAEKYAGKGLATVCKLV
                     GIPPVLHMGSCVDISRILDLVGAAANYLDMDMCDLPVVGIAPEWMSEKAVAIGCYVVA
                     SGIDTYLGIMPPIAGSSRAVDILTSELKDKVGATFTVNTNPKELAATIIEDIEKKRVH
                     FEALVEEKMAEKAEA"
     gene            complement(41833..42744)
                     /locus_tag="PC414_001477"
     CDS             complement(41833..42744)
                     /locus_tag="PC414_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HDF2463875.1"
                     /translation="MIKKLDDILEQLKGGEKIVLSVAAAHDKEVLMAIKDAVERNIIT
                     PILVGNEGKIREISKEIGFDLSGIKIVDKDDIKECAEIAVKLVSSKEADFVMKGLLDT
                     SVILKEVLNKDYGLRTDSLLSHVMVYELEKYHKLLITTDGGMNIAPDYEQKAKILKNS
                     IKAAKALGMETVKVACLAAKEKVNPKMQATVDADMLAKACKEGEFGENVIVEGPLAFD
                     LAVSKEASEIKGFKSEVSGDVDIILVPTIEVGNGIGKAFTYMADSKSAGIIMGAKAPI
                     VLVSRADSHESKLYSIAYGAIVAKNMK"
     gene            complement(42860..43438)
                     /locus_tag="PC414_001478"
     CDS             complement(42860..43438)
                     /locus_tag="PC414_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDF2463876.1"
                     /translation="MNINEFLTHINQRKPVSSKSEIHEFMVKLSNEAMKITSKLNSSY
                     HEPEEVRSLFSELIGKKVDESFFMFPPFYTDCGKNITIGKNVFINSSCHFQDQGGIEI
                     GDNTQIGHNVVLATLNHGIAPEKRGTTYPSPITIGKNVWIGANVTVVPGVTIGDNAII
                     AAGAVVTKNVAENTIVGGVPAKLLRVIDEIPE"
BASE COUNT        13440 a         8328 c         4788 g        16974 t
ORIGIN      
        1 tacactaaat aatatctttc acactgttgc tttttcttta ataatactaa atagtatctt
       61 tcacactgtt gccctttctt taataatact aaataatatc tttcacattg ttgctttttc
      121 ttaaattaca ctaaataata tctttcacac tgttgctttt tctttaataa tactaaatag
      181 tatctttcac actgtttctc tttctttaat aatactaaat aatatctttc acattgttgc
      241 tttttcttaa attacactaa ataatatctt tcacattgtt gcttttcttt aataatacta
      301 aataatattt tccacattgt tgctttttct aaacaactat aaataatata ttaaaccctt
      361 atttcttttt ctaataagtt aaataataca ttttacacat atctatttgt cttgcacata
      421 ataaataata cattatattt ctatctaaat ttagattcta aattaaatag tataaattat
      481 attcttcaat aaacaagttt tcaaatccaa gattcgctct ctatctttaa actttattgt
      541 aaacataatt ccatcttttt taattatttc tccttcatga aattttttat ggtatacctt
      601 atcccctaca ccaatactgt caatttcttt tttcgttgga gattttatat cattaataaa
      661 taatgattta tcaactttct taccatactt atttaaagta gagcttatac ataacttttc
      721 ttcagctctt gtaatagcaa catacataag tcttctttct tcttctaatt gttcttcttt
      781 tttctcttcc ttacaaatat catatgattt ttcatgaggt attgttcctt catttacacc
      841 tataatataa acatttctaa attctaatcc ttttgcacta tgcatagttg taaatatgac
      901 accctcagtc tgcttattct tgttattttc cgttaactca gtctttactc tatcaatatg
      961 ctctaaaaac tctgtaacag tattaaaatt agttgctgaa ctctcaagtt catttaatat
     1021 ctctacaagt ccattagtct ttatctttct gttgctacaa taatctaaaa catatctatc
     1081 ataatcaaga ctagttctaa tataagatat tgcattttta ggatttaaag tatttaaata
     1141 acttaaatct atttccaaat catttattgt tttaacttgt ttaggatgaa gattacactt
     1201 atttattaat gctgttataa agtctttttc atccttaacc atatttatac tgtctttgga
     1261 tatatatcta aaaggcttgt ttataattct caaccaatct ttatttgatt ttggattaat
     1321 acctattctt aaataggcta gtatgtcttg acttgcccaa tggtcatata ttgtaattac
     1381 tgagtctttt actacaaatg gaatcctcat atccataaat acatctacta atgctcttga
     1441 ctgtatatta gttctataaa ttactgcaaa atctgaatac tctacataat ctttttgtat
     1501 ttcgtcaatt atttccctgg caatatataa ggcttcttcc tctgaatcat gcggagaaat
     1561 ataggtaaca cttcctccat ccccctgact acacttaatg attttttcat atctattttt
     1621 atttttgtcg ataagtctat ttgcagtatg aactatctca cttttagacc tgtaatttat
     1681 atctaatatt atttttttag tattattaaa gtattgttca aattcaagca aaaaatctgg
     1741 tctagcacct ctaaatccat atatactttg gtcttcatct ccaactgcaa atatattgtt
     1801 taatgggcta caaattaact ttaaaacttc aaactgtact ttatttatat cttgaaattc
     1861 atctatcaat atatatttat acacatttct aaccatttct agtgcagact tattgttcaa
     1921 aagtaaataa taagttctta taagcatatc atcaaaatct attttattaa ctttagattt
     1981 ttgttcttcg tataagttgt atacttttaa aaactcatct tttgttaata cttctgaatt
     2041 aaattcattt ttatccataa gttcattttt tacataggaa atttcattta taacttgtcc
     2101 tatattctca tcatcatcag cattctctat atttaaactt tttaggattg ccttgattgt
     2161 cattcgcttt gacttttcat caaatatact gtctaagttg tatctttcaa agtatcttag
     2221 aattttaaaa aatacagagt gaaaagtacc ataggttacc ttattaagtc ttatgtcatc
     2281 acttaaattc aaagctctat ttttcatttc tattgaagat gccttggtaa aacttattgc
     2341 caagattctt gttggtgcta tgtttttgtt tacaaccata ttggctattc tatatgtaat
     2401 tactctggtc ttacctgaac caggccctgc aagtatcatg caaggcccat ctatatgctc
     2461 aactgcttct aattgatttt catttaactt tcttatatct atatttgttg tgattattag
     2521 ctccaccacc ttgatttata attatattat tttcttttta attataaact aaaagttcat
     2581 aaaaataaat ttaatttttc ctctattaaa ccacatcatt cctattttca tgacatatct
     2641 tgagttcttt tttaattttg tcgcttatat cttcaggtat aaatgatggt ttactaatat
     2701 agtagccttg catataatct actccaagat taattagtgt ttccatttca ccttttgttt
     2761 ctacaccttc tgctatcact ttaatatttc tttcgttaga atatgagata agatttttta
     2821 atattttctg tctgttttca tctttatcaa tacctcttac aatagacata tcaatcttta
     2881 catagtctgg cattatatat aatagtacag catctccgtt gtaacctgtc ccaaagtcat
     2941 ctaaagcaat ttctatattc caatggtcta tatatgcttt ctttttctca ataaaatctt
     3001 cattaatctt atcattctct gttatttcta gtactactct atttaaatat tcaccatata
     3061 aatcttcaat ttcctcaaaa tctacttttg acaaattatt attaggaata gaatttaaga
     3121 acagtttgca atcgccaaat ttttctttgt attttttaaa tgatttaagt gctttaaacc
     3181 atgtaatacg ctcaatttga tataatttag actgtgaacg agctaatcta atgacatcta
     3241 ttggcgaact aaaagttttt attttaggtc tcataagtgc ttcatatgca aaaactttac
     3301 ctttacaatc tacaataggc tgaaatgcat actcaactaa ttgaccatca attaacttgt
     3361 ttaattcttc tctattactc aataagaatg aatccttatt atatatatta atatcaaatt
     3421 cactaacttc acccttgatt gtatttttaa tttgatacat agcaaaatct gcgtatctca
     3481 ttaattcatc atagttggtt gagtcatcag ggtaccagga aattcctgct gatgccctta
     3541 ttttaaattc tgttccatca tgaagtttta ataaggtatt attcatctgt ttcttaacag
     3601 aattaataat atctcttata ctatctttac tttcatatcc atatataaat acataaaact
     3661 catcacctga aagtctagat actataccat tccatgatat aaacttctta agtatgtctg
     3721 cagcacacct aatatattca tcaccagcat catgtccata tgtatcatta atatatttta
     3781 gattatctaa atcccacata ataaatgctg ctatcttaag ttcatcttta ttcttaaatt
     3841 tttcttgtat ctgtgaatga aaagctctcc tatttaaaag attcgttaaa atatcaaaat
     3901 ctctttcata ttctatcttc cttttctcaa taaattcttt tgttacatca acaacaacac
     3961 caagaacctt tagctcatct tcaaagatat tcattcttat ccacttaagt tttttatctt
     4021 tagattcaaa cttatatata tatgtatttt ctgtttcata actttcttgc aaatatttat
     4081 ctagtcccct taattttttc aggaacttat ttttttccat atatccatat tcttcaaatt
     4141 tgatttcagt agtaaacacg ctaaaaaatt cttttgtaca aaaaacattc tctgcgtttt
     4201 tgtataactc aaaagctcca attggcatat ctaccatttc aataatctgt gataattttg
     4261 acgaagaatc tgcaacattt gcactgagca actctatagc tgaagataat tcatctattt
     4321 cagatatatt tatcttatca agatttactg gttttgatgg attactgttt ctaactttgt
     4381 tggctaaaag aacaattggt tttgtaaata atcttgatgc aatataaata cctattagac
     4441 ctattaataa agaaattgct atagatatta taactaattt ctctaccctt tttgaaaatg
     4501 acaaaagact tttttcttca ataataccca aaagagccca acgttcattt tcaaatggag
     4561 tattggagtt atataaatct aagtattgta cactagcata aatgttgcca ttaccacatt
     4621 cattatcttt atttttaatc ttatatatat ttttatacga ttccttctgc tctaaattag
     4681 aatgtaaacc atcccaaaat ttttctttta atgagtatcc atttgaaatt acattttcaa
     4741 aggccatatc attatttcta tctacaccta aaatataatt acccttttta ttcccattta
     4801 tctctgagta tggaagtaat tctcttaaat aatttacact taaatctatt ccaagtactc
     4861 catatacagt tccatcttca taaataaggg gaactgtata tgtcataagc ctatcacctg
     4921 catttgtcaa taaaaactta cttccccagt atcctaaatt agaacttgat acatttggat
     4981 tatcaattgc agctctaaat ggtttataaa aaaaatcatc tccatcttgc tcattttcac
     5041 ttttaaatga aaacttaggt tcccaataac tgtccataga tattccaaaa cttcttgcta
     5101 tatctgatga acctctctca attaacaaat cagaattatc atttggatta aacttagggt
     5161 ctaaatccct tatatacaat cctgtcctac ttatttcacc tttactatct attgacttat
     5221 cgttaatatt tttgtcactt aggattataa aagctcctgt aacagaattt tttctcaaaa
     5281 gatatattaa atcatttgat atgcttttta taatatcttt gttaatttct tcattgttgc
     5341 ctacatcctc aattaaggca tttttttcat ttaatacttt ttttacagag gaattaattg
     5401 tagcttccgt ttcacttgta tttgaccatc tttgtaacat ttcattttgt atataatttt
     5461 tacgattaat tactctttca tttaagatgt caaacgaatt atcatttaat ttcttaatag
     5521 ttcctcccca taaaattgtg gcacaaaata aacttgtttg aattagcata acaataatta
     5581 ggggaaatat catctttttc attattgaat tgttttttct tagattgttt tccataccca
     5641 ttccacctat acctttgtgc tcgtcaacac actaactgcc aattcatcta tttttttata
     5701 gcattttcaa gctcagcctt aaaactttca aaccaagttt taaaattttt atcattatta
     5761 aattgtgcta ctgcatcctt cttagaagtt ccacttttta ctaattcatt tattttattt
     5821 gaatcttctt tagctttatc aagcattgaa tattcaagta tttgtctagc tttagttcca
     5881 ccatcaaatg ctttattagt atataaatta caactgttaa tctgttcaat agatgaggct
     5941 attgaacctt caactttatc agacaaactc aaattatctt tcttcataac ttcttttata
     6001 acatccaaat cattcgtttt tttctttact ggtaagtatc cagaagcaat agagaatttt
     6061 atattaggtt ctttatctgt aaaccatttt aaaaattcta ctgatgcata ttcttcttct
     6121 ttagtagatt ttgttacagc cattcctgca ccttgttgta cagcatattt tccttctcct
     6181 ttaaaacgtg gtgcttgaaa aacagatgtt tcaatattat aactctcatt atcatttagc
     6241 attactctat ctggaaagta tgctgctcct gaactagaac ctacaagagc taatatatca
     6301 cctgtttttg catcatcaga acgaaactta ccataagaag caaaatatcc atttatatat
     6361 ggtacataga aattatccca tagttttttc ataacttttt catcaatatt tagcgtaact
     6421 ttaccatctt taacagaaaa aatttctact cctagttgtt tacttcctat aataatataa
     6481 tttgctaaag catctcttcc aaaaaatgct ttaccatcat taggctcttt cgttaaacta
     6541 tctgtccatt cataatattt ttttgatgtc tctactaatc cttcaattgt ttttatatca
     6601 tttatatttg aatttgtagc ttttgcaaat ttatcccaat cagttttatt taacatcaaa
     6661 atttcagttg attttgcaat tgggaaaagt ttaaactcat ccttagaatt tattctgcct
     6721 tcttctacat attcgtctat gtattcattt aattcttctt tacttatgta cttatccata
     6781 tctactagta accctaattt atccatttca tatgcagtat ctgagtatgc tgcaaatata
     6841 tttggtacat cttcagaacc aactttttta tttgcagaat ctaaaacttt ctctgataaa
     6901 tcaactacat taccttgact gaatgcttca acaataattc ctttttcagc acctacagtg
     6961 tcattaaatt ccttaacaag cttatcaaaa gccattttct gctgtccatt ataataatgc
     7021 caaatttgta ttgatgtcgg attttctggg tctaacttat aattatcttc cttctttaaa
     7081 ctacatcctg taagtaataa aacaatcatt gatgtgataa ttaattttgc aaacttacac
     7141 ctattcataa aatctactcc ttcaaattat aaaatttata atagcattaa aactgtaact
     7201 tcactattta tattaattat agaacgttta ttgatatatt taaacaggat ttacaacata
     7261 tttaaaagca attaagaaac atactattac aaatatagaa aataaagctt ttacataatt
     7321 tttatttcta aatgcaccaa atgacaccaa tccttgttga atagctactg taattactaa
     7381 aagtatcatt attatattaa gttgaaccag tttaaataag taaagcaatg aaaacactac
     7441 tagaagtaca cataatacaa tactagattt attaataata tcatcaatct tatctacttt
     7501 cttagattgc ttattaattt tatttaattt ttctttattt aaattctcaa cttttttatt
     7561 atacctttct cttgttttat ctttatattt agtattttta tttttatttc ccatttattt
     7621 tccatcctct ataaattttt taattgagct tatacattat ttaaatatta tatatttaag
     7681 ataccatata ttcctacaaa aaggtaactt actatgtatc tatttttgat tttcgcataa
     7741 aaaagccatc tcctttagaa ttaattctgt attgagacag ctaatttaac tatttatcct
     7801 ttgaaacatt ttctacatta tcatatttac tatcaactaa aactatatca actcttctat
     7861 tttttgacct accttcttca gatgaatttt ctgctattgg tctaaactca ccatatccta
     7921 cagcagataa tttatctggt gctatacctg cattgtcaat aagaagttga acaacattgg
     7981 ttgctctcat aactgataaa tcccaatttg acttaaactc tgaatttttt atagacatat
     8041 tgtctgtatg gccttctact ctcatgtaac tattcatttc atttaacatc ttacctattt
     8101 gaataatctt atctctagaa ttatccttca caatagcttt ccctgtatca aatagtattg
     8161 tatctttaag gcttattact aatcctctat cttgaactct taatgatact gaattagcca
     8221 taccattttc gtttaaatac ttctgaattt cttcaccaat ttttttaaac ttagcatttt
     8281 gtaaagtatc taaatcatta cttcctggtt caactactgg ttctatcttg ctattaccac
     8341 cttcaataac tccagaagaa ccaccaaaag cagaatttag agattgagat aattgtttat
     8401 atttctccgc atcaacatta ctcatagaat acattattac aaagaaaatc ataagtaatg
     8461 ttatgaggtc tgaataagtt aaaagccatc gctcattgtt ttcttctttt tcatcttcat
     8521 catacaacat aaacattcca tccttttata ttattcagct ctaccaccaa tttcttcaag
     8581 ttcaattagt tcttttgaat ttaaaaatat ttttaatttt tctgtcatta aattagaatt
     8641 atcaccctca agtatggata atattgcttc tatcaccaaa agtctttcac ttacttcttg
     8701 ttgatttata gccttaagtt ttgatgctat tggtaaccat attaaattgg cagaaccaac
     8761 accatacaaa gtagccaaga atgcaacaga aatttgaggt cctaatgcat ctggattact
     8821 tgaaagatta cctaaaatat gtactaatcc cataactgta cctattatcc ccagtgtagg
     8881 tgcatatcca cctgctgaat caaaaattgc tattccttct ttatgtcgtt ttgatgttat
     8941 ctgtatctgt gattccaata tatttcttat agtaactgca tccactccat ctaccataag
     9001 ttcaagccct tttctaataa aagggtctaa ttcatcattc tcacttattt ctttgtcaat
     9061 actaagaagc ccctctttcc tagcctttgt tgcaatttct ttgaaataga ttatattttc
     9121 tatgttatta ggctttttat ttttaaaagc taataacatt atctttatag actttttaaa
     9181 cacagggaat ggagtcgaag ctccaacagc tcctatagtt ccaccaaaca caacaagtgc
     9241 tgatgaaaat acaaataatg ctccaacatg tcccccatca ataacaaaag caactaccaa
     9301 agatgctatc cccactatga aaaaaattaa agttgtcatc tattccctcc tttctttagt
     9361 tatacatatc taatcattta tgacattata aaaagactat ctttaataaa aatagccttt
     9421 aataattggc ggagaaggag ggattcgaac cctcgcaccg gttacccagc ctaatccctt
     9481 agcaggggat cctcttgagc cacttgagta cttctccata tctctatact aataaattat
     9541 ataataaaat catattttag tcaattgttt tataaaattt gagttatttt gaattattaa
     9601 acatctattt ttactttttt gcttaaatgt aagttttaaa tatggtaaaa ttatcttgca
     9661 taacacttat aatattattt gataaatata catacattaa caaatatacc taaactctga
     9721 cttaattgta agttatgcca aaactaaaat aatactagtc caaggaggat attttatgtt
     9781 accacaggaa agatatgaga agattttaag tattctcaaa tcagataata taattaaaat
     9841 aaatgacata gttcaaatgt ttgatatatc aatagaaact gcacgaagag atttagccca
     9901 tatggagagt atcggtcttt taaaaagagt ttatggtggt gctataccag ttatagcaag
     9961 tagcgtagaa cttaactatg ttaaaagaaa aaaaataaac tcaaacgata aaaaacagat
    10021 tgctattaaa tgtagtgagt ttataaatac aaatgatact atttttatgg atactggaac
    10081 tactgtactt gaagtttcaa aaaatttgaa agataaaaag aatctaactg taataaccaa
    10141 ctcaatttta gttgcaaacg aacttatgaa tactaatata aaagtatatg tgcttggggg
    10201 ctatcttaga gaaactgaag gttctttttc tggtccgctt acactgtcag ctattgaaca
    10261 atttaatgtt gataaagcta ttttaggcgc tggaggtatc actcttgaaa atggaatctc
    10321 tgactatcac ttagaagaag cacttgttag aaaaaaaatg attgaaaggt ctagaaaatc
    10381 tatagtagtt gtcgaacctc ctaagtttgg tataaattca tttgcatcag tagctcctat
    10441 agaacatata gatactatta ttacctctgc tagtatagac cataatataa taatagagtt
    10501 ttctcaaaaa ggaatacctt tagtcatagc tgataattag cttatatgat tgatttttag
    10561 ttccttgatg taataaatta aaattaagga gccaaataat tatttattta gctccattta
    10621 tgtctaaact atactttata ttttaattat gtaactactt tcagttagct tatcattttt
    10681 agttatttga ttgccatttt atttaattta taagttttag ttattcgatt attttttaat
    10741 tgtctgtctt tttaatttat ttttagttat ttaaactcct actttaaaat aatttaaatt
    10801 tgacttaagt attgtttatt ctacactgca tctttcttca ttttaatact ttgttcttct
    10861 tccaaggtta aatcttttgt cataagtata gcaagtgcaa ttgccaatac tcctccaacc
    10921 aattttccaa ttacaacagg tgtaatcatc tctggaacta ctcctgctgt aaatcctaaa
    10981 tggtccccca aagctgctgt agcacaaacc agccaagcag tatttactac tttccccttt
    11041 ggactcatat ctttcatcat tttatatact ggaatactat tggctaaagt aaaaactaat
    11101 cctgctgcac tagtggcatc catacctaat tttccaccca ctattgtaag tggtttatta
    11161 agaactttaa ctaataaatg taatattgga aaagtaccta ataacacgat tgcaatactt
    11221 ccaattactt gaataccttc tcttataggt gtcatgcctt ttataagtac tatacctgtt
    11281 atttcttgaa atgctgctgc tcccaaacct atcgttatca atatagatat aaattttcca
    11341 aaaattaatg acccatttat cataagagta gaattaaatt ttaaacctac tgccaatatt
    11401 actgccaata ttatcactgg tatcatattt ataattacca ttgataagtt aaatcctgct
    11461 attaatcctc ctataatacc tccaaagggt attgttatca agcctatcag taaccctttt
    11521 gcaaagaatg gtctgtcttc atattctata agacctagtc ctactggaat tgaaaatact
    11581 aatgtacatc caagcatgga tgacacaacc aatcctgcaa gcattcctgc ctctttattg
    11641 tgtgcaagtt ccatagccaa tggataccct cccatatcat tcgctaaaat agttgcaaac
    11701 atagatgggt cagctccaca gagattgaag attggtatta ttataggacc tagtacatct
    11761 gaaattactg gagctaaaca aactatacca accattccta aagccaatgg acccattgaa
    11821 ttaaaccctt cttcaaactg ctcgccaagt ccaaatttat ttccaattat tctatctaac
    11881 gctcctaaaa gcactcccac agccattatg tataatataa ttttatcaaa gggcattttt
    11941 aattcctcct atacatgtaa aatactctat tgtaacttaa ttagcacaca cctttttggt
    12001 atcgttttcc caattcatag ctctttttac tgcttggttc catccaagta atatttcttc
    12061 tcttttttga gtagtcatat ttggaatata ttttttgctt attgtaaatt cacttctaat
    12121 ttcatcaata ctgttccaga caccacaaga aagacctgcc atatatccta ctccaagtac
    12181 tgtcgtctca ggattttctg caatttcaat tggtatacct aatatgtcgg attgaaattg
    12241 cattgtaaag ttatttttag taattccacc atctactctc aacattttta tttttttccc
    12301 tgaggctaag ttcatagaat caacaacatc tttgacttga agtgcaacag actcaagagt
    12361 cgctcttact atatgctctt ttttagtact tcctgttatc ccaaccattg tacctctagc
    12421 atattggtcc caataaggtg ctgctatacc tgcaaatgct ggtacaaaat atacacctcc
    12481 agtacttgat atggaatttg ccatgtcatc agtttctgaa taatttttta taactccaat
    12541 gccatctcta agccattgta ttgcagcacc tgctatatac actccaccat cataagcata
    12601 tgtaacttta tctcctattt tccaagcaac agttgtcaaa ataccattac ctgctatatt
    12661 tagttcctca cctatattca tatataggaa acatcctgta ccataagtat ttttaaccat
    12721 tcctggttca aaacatccat tgccaaataa agctgctggt tggtctacca ttgaagcagt
    12781 aatttttata gaaaccccac aaaatgcctc cttatctgta gttccaaaat caccactcgt
    12841 aggcattata tctgctaaaa tacttctagg tactcctata atgtcaagca actctctatc
    12901 ccaatccata tctcttatat taaaaagcat tgttcttgct gctgtagata catcagttaa
    12961 aaaagatttt cctcctgtca ttttccatat taaccaacta tctaatgttc ctgcaagtac
    13021 ttctccattt ttaatttttt cttttacacc ttttacatta tctattaccc atttaatttt
    13081 tgtacctgag aaatatgggt ctataattaa cccagtctta tctattatct tattttctaa
    13141 acctggtata ttctttaaat tttccacata atcataagtt ctcctgcact gccacacaat
    13201 agcattgtaa agtggtttac ctgtattttt atcccataac attactgttt caccttgatt
    13261 atctatacca attcctttta tatcactttc attgattcca ttttcaaaag cctgttgtaa
    13321 tacattatta ctcacagttt ttgtattatt ccaaatttct atagggtcat gttcaacata
    13381 tccagattgt ggatatattt gagtatgttc catataatcg ctaaaaacaa actctgattt
    13441 ttcgtttatc aatactgctc ttgttcctgt tgttccttgg tcaattgcta atacatattt
    13501 cactttaaat gtcctccttt attttcagaa ttaataaatt cttttttatt ttttgaatat
    13561 ataatctatt caagttattt atattttata tcatcatgtg gatttatgca atattcatta
    13621 caatattttt tgttattttt aattatagtg attgaaaaag tatttttatt gaaatattgt
    13681 tgcaatattt attattttat tctataatct agttaaaata acacataata ttttaatatt
    13741 tacacataat cagtcattca aaaatttaaa tttaactttt taaggaggct ttacaatgaa
    13801 aaaaacatat ctattcagtc ctggaccagt aatggttaca gataaagtca gaaattcact
    13861 tcttcactat gatatatgcc atagaggaaa tgagtttatg gatttattca aagatacaca
    13921 acaaaaaatc aataaactat ataatgcaac ttctaattac tactcattaa ttgtatctgg
    13981 ctctggaaca tctgtaaatg aatgtgtact atcttctatc tttgacaaag aagatgcagt
    14041 tctgctagtt agtaatggtg tttttggaga aaggctagaa gaaatattat ctacttataa
    14101 tgtaaaaaca tataaaccta attttgaatg ggcagaatac ccggacctag acattttaga
    14161 aaaatacata attgaaaact ctgatataaa agtaattgct atggtgttcc atgaaactag
    14221 ttcaggaatg ataaatccag tccctgaact aggaaaactt gctaagaagt ataacaaaat
    14281 attttttgta gatgcaataa gtgcctcagc aggtgaatat attgatgtgg aagagtttaa
    14341 tatagatata ataacaggtg ttggtggcaa agctcttgga gcatttccag gttctgcata
    14401 cctttgtgct aaagagagta ttttacaaaa tattaaagaa aatcaatgta agaatgtgta
    14461 tcttaatctt tataaacact ataaagttgc taaatcatct tctcaaacac caaatacacc
    14521 taatgttact ttaatatttg cccttaatga agctttaact gagtttttgg aagatgacag
    14581 taaaatagaa agatacaaag aatgttctgc tattttaagg tatggtatgg aagaattggg
    14641 tctaacattt ttacttccag ataagtatat gtctaataca gtaacatctg tatttctacc
    14701 taaagaaatc aatgtaaatg agtttatatt agggctagaa agggaaagtg gctatgttgt
    14761 ctatccagga aaaggcaaat tcctagatgc taacatattc caagtagcaa atatgggaga
    14821 aatatatcca gaagattgtt ataaattctt agatatctta aaatcaaaat tggagataaa
    14881 aaaagttata taacagtttg tttgtaaaat cctacaatat atcagtataa attaaataga
    14941 ctgatttttt taaattttcc tataataaat tatattttat atttttgtat aagggtgaac
    15001 tataatatgt aaagttcacc cctatttcaa atctttttcc ttcaatataa tctttttctt
    15061 ttaatttatt ttatattttt gacgcaatcg ttttttattc aatcatttta tattcttgat
    15121 acaatcattc ttacacttaa ttattttttg tttttgacac aatcgttttt tgttgaatca
    15181 ttttatattc ttgatacaat cattcttaca cttaattatt ttttgttttt gacacaatcg
    15241 ttttttattc agtcatttaa tattttcaac acaatcattc ttacattcta ttattgtttg
    15301 tttttgatac aactgttttt attcagtcat ttaatattct caacacaatt accttttcat
    15361 tcaatcaaag taatctttaa gcattatttt tcaagctcat caaagtaatt atatacgata
    15421 acctcacctg tctctttatc tgtagtcttt ataatgccct tagctccatt tacctcttct
    15481 tttgtaaatt catcatctaa cacaactttg aatttatctt cctttccata accttgttga
    15541 aattcatctc tcaaaattgc atttttttct gcttttggct tcaactcaaa ttcaattcca
    15601 gatttcattt catcaaaata atttaatact acaacctcat tattcatttt attagttgtc
    15661 tttataatcc ctttagctcc atttacttct tcttttgtaa attcatcatc caatgttata
    15721 ttaaatttgt cttcttttcc atattcctct tgaaaatccc tcttcatttc attgtattta
    15781 gtttcaaact ctttactctt gtttaattcc atagctgata catttgtcaa tgatgctcca
    15841 attgccatta ctgttaatgc cattactata ccacttttaa atttttttac tttcatatct
    15901 tacctcctaa attaaatgtt ctcaatgaaa ttatataaag aaaatcctaa gttttacagt
    15961 ttcattcttg taactaaaat tactattatg taaccaaact ttttgcttca actattcctt
    16021 aaaagttgct tttttgagga atagttgaag ttaaataatt agattttatc tatatttata
    16081 gatttaaaat caaatttatc catataaata aatacttatt tttgcaaaat aaaaattttt
    16141 aattactcca atccttctta tttacttaaa atcctatgat aagaaattta aaagtcttta
    16201 ccttctttaa caataattta tttactttta acatacaaaa tatagagagt atatcaaaac
    16261 tattttactg aatattctaa aaataagtac cccctattag tatatatatt tacatattaa
    16321 aaaaaagttt taaataaagt tatatttttt acaacagttt tgtaattaaa aacatattga
    16381 aggtaaattt aaaggtacta tatactataa atatgtcgac acaataaatt taaaaaggag
    16441 aactaaatat gaaaaaaaga ttaataataa tgatattgtc tgttgtctta gtactatcaa
    16501 gtatacttac gatatttgca tattcaaata ttaaatataa taataacaaa ttgatatatt
    16561 ctaatatgat agataaaaaa actcaaaata gtgtaaagga aatcctaaaa gaaaataaaa
    16621 ttaatgaaaa agacatagat acatttatta aatcagtcaa caattataat aaattgcaag
    16681 ttaaaatact tcaaaataat ataaatatat ctaaatctgg atattcttca atcaatgcaa
    16741 aacaagttcc atataaccta gaaaaattgc aagataattg ggtgaaaaaa ttccctgatt
    16801 atatggatgt aaattgtaga ataactgctt ttagactttt taaagatttt ataaattcaa
    16861 ataagaaatt tactggagac tctattgatt tgaatgtcga cttggacact ataatgaata
    16921 ataaagatgc taaatttagc accaaagatg tagaaaaatt tatcaacttc ttttctgcaa
    16981 taccagctaa agatacagat gatacaatca aaattgcaga acaaataaaa aatgaatgga
    17041 aaaaaagaaa aatatctttt aaaaataata aaaatatatc tattataaat ggatttcttc
    17101 gttatccaga aactaaaaat gtatttatag gtcatacagg catatgtatt aaaactaaaa
    17161 atggcattct atttttagaa aaatacggtg ttacatcacc ttatcaagtt actaaattta
    17221 aaaataaaga agatgtaaaa aattatatgt ttaatagatt gaagatgtca gaaggtgaaa
    17281 ttgaattacc tgaccctata ataatggaaa acgataaact tatgaaataa taactttaaa
    17341 cgcaaaatag tatttataat tactaagtca atataataga gaaggtctat agatttgtgt
    17401 aaatacaagc ttatctacaa tcttatataa gaatgtttat ataaataaga tagattaatt
    17461 ctaagtccta ttattctatt gaattttatt attattttat ttagattcta ttgttatttt
    17521 acttagaact tattattatt ttgtttaagt tctattacta ttttcaatca tataaattta
    17581 caaaagactg tacataaatt tattttatac aaacttatct acagtctttt gtgtgaatat
    17641 atctaaataa tatcgtattt atttatttcc tctgcacagt gagaatcaga acctaaaact
    17701 aatggaatat cgtatttttt taccaagtct aataagtatc catctatata aggctctttg
    17761 cagtactctt ttctatttcc agaaacatta taatcaagtt cataaccttt ttctttaatt
    17821 aactttatta actcttcaag aattttattt cctttataat cgtatggaaa tacctgatta
    17881 aactttctaa ctaaatttaa atgacctatt ctttttggtt tatgtatacc taaatctgaa
    17941 tttacagctt ttatcaatgt ttcataatac ttattataaa cagcttcaat acttcctaat
    18001 ttgtcgatta aattttgaaa ctcctctaca ctgtaatcta cacaataata atcatcatca
    18061 atcttaatta tatgtactga taaaagagaa tcctctaatt cctttccata tttatttaaa
    18121 ttctccttta tcccttcttc gtatccttct ataaaatcca cttccgaccc aacatttatc
    18181 ttgattttac cctcatactt ttctttcaac ttcttgacat cattaaaata ctttggtaaa
    18241 tcttcaatac tcatacaact atcattttca ggagcagggt ctctaaatcc atttgcatac
    18301 ggaaaatgtt ctgtaaatgt catttctgtt attccaacat ccattgctct ttgtacatat
    18361 ttttcaaaat catccttact tccatgagga caataaggtg aatgtatatg accatctttc
    18421 ataaataatt cactcctttt ttaaagaaaa aatctaatat aaatgcataa atcttttaac
    18481 ttcaaaataa tatttttaat catgctaatt tttattatat ctaatattag agtaatttta
    18541 aactgccttt taagtatctt atcattagta ctattttcaa cttaacatta atattatttt
    18601 ttaacttaac attaatacta tctttaaata gtaatactca atatataatt acttaacata
    18661 aaataactca aattgcttga tgaggctttt gcagtattta aagaatatat aattatttaa
    18721 cattaaataa tccaatcttg taataaaatt tactcattaa agtatattac agaaaatacc
    18781 tcaaaataaa ctttttagtt catgatatac tcttttttgc atgaaattag atatattttt
    18841 atcatttcaa caatgaaagg gaggcttata tccatcttta gacaacccct tagataaaat
    18901 ttttaataat taaacatcac aaaatataaa aatcgcttat atcaaacgta taagcgatta
    18961 attatattta catatgtttt ttagcctcta acaacatttt attagcttct tccatatact
    19021 ctatagcctt ttcaatactc tttgctgttt cttcttttcc aatagctcta gccttttcaa
    19081 cccactctct aaatccttct tcatgagttt cattgtgatt tatccaatgt actaaaagta
    19141 tttttaaagt tttttcatcc ttagactctg atgattcctc atgactatgt tcgtggtcgt
    19201 ggtcgtgagc atggtcatgt ggatgttcat gacatatatc tccatgagta tgttcgtggc
    19261 agtggtcatg gtcatgatgt ccatgatgat atcttacaat ctctttatca aaaatcatta
    19321 gttattcccc ctgttatttt atattaaact ctttttttat aaggtttaca gaatctttta
    19381 caagtagtgc taatggttta ccttctaatc caactaactc cacattttct ggcataatag
    19441 gtaaaagtat cttaagtgcc tcactgtctg caatagcttc acttatatta caagtaactt
    19501 ctcccatcat agaattaggt attattacag aaataggtgc aactattata tttgcttttt
    19561 tagctgatac tactatagca ttttcccctg tagctccttt attagcatga gctttcatca
    19621 tagaactagt tgctatagaa ttagtcccta gtgcataaat ttctacatat gtaggtaact
    19681 cttctctcaa agaagaaact atctgggctc caatacctcc acccattcca tctatcactg
    19741 ctattatcat atgaaattcc cctattaatt aattttcttg tattattatc ttatggtcca
    19801 tcaatcttat ttctttaatt atcccatcta ttatcttttg ctctcctaat aaatccgtta
    19861 aataaatctt tccatcacaa gggtctatat taaccacatt ttccataacc ttttctaact
    19921 cattattact tttacaaatg aaagctgatg attcacacat ataaatctcc tcctaatatg
    19981 atttttatta aaaatcaaaa aaagattccc ttaggtattt caatacacca aagaaacctt
    20041 catgatttca gtttttgtat attattatac cttaagtata tcactttaat ttatgtaaag
    20101 tcaattaaag ttaacgcttt ttattatact cttcattctt gtatttttca tatagttctt
    20161 taactaattg cttctcataa tcagtcaaat cgccctccac aaactcaaca ttaaattgtt
    20221 cagctaatcc ctttactatg tttttctgta gaatatctat gtctattttt ctgccaattt
    20281 cattttctat tccacttgct tttttagctg taaatttcat agctctttct ttcaattctg
    20341 gtgtcttagt ttttattaat ttaaacaatt tttctacatc aaaatccagt attattgaac
    20401 catgttgtaa taaaacacca tcttttctac tttgagcact tccaattacc ttcttattat
    20461 taatagtaac ttcatatgat gcatgtgcat taaaacatgc agctgataaa ttttccctac
    20521 ttattctctc gcctctattt aaattatctg tttctatacc acttagattc aaccctttaa
    20581 ctagaccttc acttatatat ctataagata ggttgatgct cttatcgatt aaaggattat
    20641 cttctcctat aattatacta taggttagct cattatcatg taatacagct cttccacctg
    20701 taattcttct agtatagtct atattcatac atctacactt atctaaatct atctcatctt
    20761 ccaatttctg aaaatatcct atactcaaac aagctggttc ccatgtataa aatctaagag
    20821 ttggcttatt atgtcctttc ttatatgcag taaatatagc ttcgtcaatt gccatattca
    20881 ttgctccttc atatgattta ttatgtataa ctctccactg attcatgatt attacgcctc
    20941 catttttagt ataaatatat cctagaaatc ctctatagat ttatttctct tattgctaaa
    21001 tctatctcac tttgatttaa aggtcttgaa tagttgtata tagtacttcc tggtctttca
    21061 ttatagtatg gtctattaca atccttacaa cctcttattt caaaaggata gcctttgttt
    21121 atgtctttta aaatatcatt gtcaagcttt atactattta agtatccatt tttaaactta
    21181 aaatactctt ttggataacc tttatttatc atataagaca ttaactgtac ccttctataa
    21241 ctttctatgc ttggttgatt tatcttttcc attttagttc ctctaactgg agtaaatgca
    21301 aataaactaa tcgttacatc attttccttt aaataagtat ataaattgta tatatcttca
    21361 tgactttcac ccataccaac tattatatgt gtacttattt tattgggata tgattttgac
    21421 atttcagtta taaattttaa cttttcatca tagttatttc cttttatttt ttcatatagt
    21481 tctttatttg ctgcatctat tgctattcct attttatcaa ctcctgctga aaaaaacttc
    21541 tttatctgtt catcattttc taattttgca gatagtgaaa ttggcatgtc tatcttacca
    21601 cttatatagt ttataaagtc taaaacagag ttatgtgctt cttcacttgc cattgattga
    21661 atacatattc tctttatatt ttttccttta taagctttta aagcatctaa tatttcttct
    21721 tttgaaaact ctggccaaat tactcttgat aatttatctt ttctagtact actttctatt
    21781 gattgagaac aaaaactgca tttatttatg catttttctc caatcattat atatgctgtg
    21841 gtaggaggta tatcgctttt tttgtttaat atcccaagtt ctattgctgt cccaactgat
    21901 agtctaatca taaaacgcaa cactccttac actcatttat tgtcatattc atttctatag
    21961 ccttattctt tgcagacctt gatggtaata ctattctatt gactccacac attatagata
    22021 attggtctag ctcttttcta tacactcctc ttggtctcat acaacctaga ttaatttcag
    22081 tatctggtaa attaattcta gcttcacata aaatatctgc taccccttct aactcaggag
    22141 gttctacatt ttcatactca gtcccttttg ttggaataag gactataaat gtaagcttat
    22201 taggaggatt tttttgtagg tattctataa tttcatattc accctttatt tgtcctcctt
    22261 ttaaaccaat acatatatgt ggtgctactt ctgtatgttc ttgtattgta ttatagactt
    22321 caatataatc ttctttactc tttttcatct tataaacttc tcttattgtc tcatcatcaa
    22381 acactaaatc aaaagaaact atatctaaat acttacacaa ctctacaatg ctatctttat
    22441 ccataagacc taaatgagca ttcaatctat atccttgctc ttttaagttt ttaatggtat
    22501 taatatgagt atttataggg acatctccct cataactaca accgccactc aatagaaagc
    22561 tagttatatt ccttgattgt actttttcct catattcttc tattggcacc atattgttta
    22621 agtagtgacc gttgcaatgg gcacatttca aactacattg attgcttgta gtactaagag
    22681 ctagtgtctg gttaggataa gcaaactcta ttttatttcc aaaattcttt aatttaatat
    22741 caaatgctat tttcattttt tcagatagat tcttattcat agtttattcc ttcttaagta
    22801 cattttactc ttctagtaat ttctttgctg ttgggaactg ctctatattc gtatgtggta
    22861 agaaacatgc agaaataaat tcatccatat aacttggata tacactaagt tcaacataag
    22921 ttatttgatt acctatttct tcaagtttat tttttgcatc tttacttata agcgctaagt
    22981 aagaaccaac aagtgaacta ttccctatat aagtaaattt ttctctatca atatcaggaa
    23041 gtaaccctat aattattgag ttttctatgt ctaggttgtt tcctatacca cctgctatat
    23101 aaaccctatc taatatacta aaatccatac ctaaactatc tacaagagtg tatgcgcctg
    23161 aatatatagc tcctttagct ctgatgaaat tatctatatc aacttcattg actactatat
    23221 cattttctaa atcaaattct tctttaaatg ctaatacata ctctcctatc tcatgctcat
    23281 taaatctaac tctcttatta tctaaatccc tgtatatttt acctcttcta tctattacac
    23341 ctttagtaat catttgacag attaaatcaa ttataccaga accacatata ccaactggtg
    23401 cacattcatc aataatcttt aatgttggct ctaaagtatc tttatcaata attactttct
    23461 ctattgctcc tgctgatgct ctcattccac agcttatccc tccaccttca aaagcaggtc
    23521 ctgctgaaca tgcacagctc atcatataat ctttatttcc aaatacaatc tctccattag
    23581 ttcctaaatc tataaataaa acattttctt cacttgacca tatccctgct gataatacac
    23641 ctgctgttat atctcctcct acataacttg caacagatgg agctaagtac acatatgcac
    23701 tgtcatttac aaaaagacca acattttcac ccataagttt tggagatttt aaaaatggtg
    23761 gtatgtaagg ttcttgtctt aagaagtctg tatacacacc caagaataaa cttgtcatag
    23821 ttgtatttcc ggcagccact aatgtaacta catcttcctt atctattcca tttctttcat
    23881 atattgtttt taatagtgga tttatagttt cctcaactat agctttatga agtgtctcta
    23941 atccattttt cttagttgaa tatataattc tatttataac atctgctcca tattttatct
    24001 gtgcatttcc agaagatgct ttatctacta cctcttttga gtataggtct actaaacaaa
    24061 caactactga agttgtacca atatctattg caactccgta taaggaattt tctttatttc
    24121 cttgttctat atttatgatt gtaagtttct tttgtttttg aacatatgtt attgtaacct
    24181 taaaatcact ctttctaaat acagttggca tttttctaag tatatccaat ctaaaatcta
    24241 tttcattata tcctaggtta tttctaacat atctttccaa tctatctaca tcacttatat
    24301 tatcatctaa atttggttct tccatttcta tgtatttctt ttttatattt gttttaaatt
    24361 gaaggttatg ttcttcaatt atttttttag ctctttcaaa aatttcccta tcctcttttt
    24421 tattactacc ttctatcttc ataccatgca tagaagatga tactttagaa ggaacttcaa
    24481 tttctatatc tgatattacc tttgtacaac aagctagtat ataaccttgc tcccactcat
    24541 catctgttat atgtcttgtt ttttctgtat caacttttcc attcaataat tttactttac
    24601 acttcccaca agacacgttt ccattacaag gagcatctat gaatatatct gcatttcttg
    24661 caacctctag taatatatcc ccctcattac aataaacctc tttattgttt ggtgtaaaac
    24721 ttacttttat catgttatcc ctcccaattt gttctatata tgataaaagg aaagtatagc
    24781 taaatttgcg tgcactcaaa ttttactata ctttccccaa ttataataat atcttaatct
    24841 ttaattattc taatccagcc tcgtattctt tatcacttaa taacgtatca agttcagcca
    24901 catcagctag ttttactttt aatatccagt tttcatatgg gtcttcattt atacattctg
    24961 gctcatcttc taatttttca ttaacttcca atacctcacc attaacagga gatattaaat
    25021 cagaagctac tttagaagac tcaactactc caaaatctac tcccttagtt acagtatctt
    25081 ctacttctgg tgtttcaaca aacaatattt ctcctaattg atcttgagca tagtctgtta
    25141 tacctatata tacaacatct ccatcaatta cttttaccca ctcatgatct ttagagtatt
    25201 ttaattctgg taataatttc atttcgatag acccccaata ttatattatt gattaaatta
    25261 cttaaatcat attaatatta tatcatatta ttttaaattt tccatcaatt acattacact
    25321 ttccatagct aatgctggat gacctttttc ttctaagaaa gctaatactc cttcagaatc
    25381 gtcagctata gtttcatcac aaaccatatc acagaagttt tctattccag tcatttcttt
    25441 aactgtagca tttaatttat cagctacata atcttttaat tcttttggca tccaaactat
    25501 tctttctggt ccaccttctg cataagcgaa ttttttagaa gatataaaat gtcttccgtg
    25561 tcccataaat cctggagttt gaactcctcc acctgtcata gaagcaagtt ctccgaaagt
    25621 cataccaact ggagtaacac ttgcaaattc tctgttaact acaacaaatc catttgcttc
    25681 tggcattata ccacagatac attcgaagca tccacaagaa gtcattgggt cttctagtat
    25741 agagtataat gtaacagatt caactgcacc ttgagatatt tggtttacag tttcatttac
    25801 tgaattccat acacctgttc tatcatctaa gcactcgcct ttttctattg gttgacaagg
    25861 acctgttggg tctaattctt tagtagcctt agcatctaac cagctaactg ctccacaaag
    25921 accaagtctt tcaggagtaa caacacaaac gtgagctggt gcaaatgatt gacataagtt
    25981 acaagaatag aaagtatcaa cactttcatc aactaatgaa gctaatcttt catctctagc
    26041 attgtactta gctattgctt ctccttttaa ttcagaaact ttttcagcat ctgtaattat
    26101 agttatttca cacttatcaa ctactgattc aaattcatct aacatcttag catataaaac
    26161 ttctcctata tgctcaagtc taaatccttt atcaaatgca tctttagaga ttcttaccca
    26221 agccatatct ctttgtccaa cgtgcattac accttctata tagtttaaga agtagtggaa
    26281 acgtctttca agaactggct cgaaatcttc ttgcatattt ttaccagcta ttttaactat
    26341 tacagcaagt ggtaatctta atacaccatc tgcatcaact tcatctatat ttggccctat
    26401 tatttctatc ttatggtctt caacttctgt tgcttctttc ttaacaacaa gctcccaagc
    26461 ttcagttctg tttccaccaa actcagcaaa catatcgctc tttctaattc tctcaccttc
    26521 aaatgctgct gcaaatgaaa ctgggatagg tatttcagta acttttattt ttattcctct
    26581 agcttctaat gaagtagcta ctatcttatc gtaatctttt tgagttaata ggttcattgg
    26641 aacttcaaga actgtttggt cagttataac tgggaatcct aaagctattg ctccagcacc
    26701 tgcagataca actaattcac ttaatggtcc aaatgcatta acaaatgcag gaactctatt
    26761 agctgtgtac tctaataatc cgtttaaatc accaggagtt aatccaccga atattaatgc
    26821 tgctcttact gcaacagata caacgtgtat aacagaagtt acatcatatc ctagaggtat
    26881 aactcttagc tcaagaccca tttttactcc agcttctata gcttggtcta taactttacc
    26941 aactaagaaa gttagtaaac ctttagattg gtaatctttt ataacttttg ctgctgattc
    27001 tgcatctgga cactcaccaa gaacaactgc aacaccaggt atatctcctg taactaatgg
    27061 tacaccaagt gatctgatta tagggtcagt tatatgacct gcacatggct cactatatgg
    27121 agcatccata gttgaatatt ttaatgcttc aattacttct gctgctaaag ctgtagctaa
    27181 accagcatta aaagcatgtt ctagtaaatg agttctattt attaaagatt ttactacttc
    27241 taaagcacct tctaagtccc ctaaagtgtt catcttttgt cctgttgcag catatataca
    27301 aggtaatgaa tatgctgtat cagggaaagc aactttatgc tcttttccat tcttttctat
    27361 agcttcagct aacatagctt gagccgcacc taaagcttgt tctgaccctg taaagattat
    27421 attatataga ttcattctat ttcccccttt aatatttaca cctatttttt attaactata
    27481 ccctaaaaac cctagaaagc aaatccacct tcatttaact ctaagaaaga gtctgcatat
    27541 aaagatgccc cgttgattat gtcacaagat tttacagttt tcattaattc tgggtcacat
    27601 ggattcatta tagctgaact aaatccatta gccattgcca ttgctaagaa tgcactatct
    27661 aaaacaggtc taacatgctt aggacatcca ttagatacgt tagataatcc tcctgtagtt
    27721 aaaagtccca tctcagtcat catttttata gcttctaaaa cttctacttg tttttcttgc
    27781 atacctttta taactaagca taatgggtca aatagtatat cagttggctc cattcctaac
    27841 attaagcctt tttctaatat ttcttggcag taagccatac gctcatcatt atctcttggg
    27901 ataccttctt ttgcacataa acctataacc attgcttcgt attcagctgc tatatctatt
    27961 aaatcaaatc ttgagccagc atcagcagag tttattattg gttttgcatt agttctatca
    28021 taaactttaa gtccagcttc gatagctttt ttattagctg tatctaatgc tataggaaca
    28081 ttgttgaact cagattgaag taatttgatt ccccatgtca ttatttcttc gccatctctt
    28141 tcggcaggtc ctatattaaa atctatatag tgtgctcctg cttctaattg ctcttttgct
    28201 ctttttaaga ttggagctgg gtctctttct gctaatgctt ttcttattga aggtgatata
    28261 caatgtattc tctcacctat aatcataaat ttttccattt ataatgtccc cctttaaatt
    28321 taatattaat agttagttaa tattttatta gtttgtttgg catgcttttt cttggtatcc
    28381 ttttaagaac ttaggtaatt ccatagattc ctctggtcct ataactacat tccatcctgg
    28441 taagttatct tctatatcac ctttaagtac tgcaactttt cctggaatga taaggtcttt
    28501 acaattagtt acttcttcta ccttactttc tttaatgaaa gcagagattg agtttccacc
    28561 aaatttacca gcagcccaag atgtaagaac tgaatatcca cctgcatctg gtattactaa
    28621 ccatactgga acttttgatc tttctatatc accagcaact atgaagtaag ttaatgcaaa
    28681 gtcaacagta actaatactg gagaattttc atctggattg ttgattggat aaatctttgg
    28741 ttcaactctc attggtcttt gtgggtcagt gtatatgttt tgtcttagtg caaataatgg
    28801 taatgcctta gcataactaa tatcatctat tactattata gaaccgtatt ttatagtgaa
    28861 tattgatgat aaagcaactt ccatcatagg gtttgaatta gataatctat ttgcaaatac
    28921 tattgaagga tatccaaatg ttctatcttg ctcttttaaa gctgttcttc taacttgtat
    28981 agcattagtg taagtatctt ttatgttttc tcctgttaca tctaatacta attctttata
    29041 tccagcggct tgaattaatt caactgtttc atataattct tctaagctac cagcttttac
    29101 acctaatggt aaacttgctc ctttaactac ttctatcata tctttgtagt tctctttagt
    29161 tgctccatat accattggtc tagcatcttt taatacttca acagcttctt taactacttg
    29221 tgcatcatca caagctaaaa tataagctac ttctaatcca ctgcctttta ttttatctat
    29281 taattttaaa tacgcatctt tatctccaca atactctaaa acagccattt caactttcat
    29341 ttgttcacca attcttacat agtcaacttt tttcatgtta gctattttag catctactgc
    29401 ttcatcactc atacaagtac agaatgaaac tgcatatcta tttctactta ctaatgtttt
    29461 ttcatgtcta aataatactg tttctccacc tagttcatac tcagatgcac cagcaccaac
    29521 ttttaaagct ttcattaaag gtgcagtagc ctctgataat tttgctatag cgtcatcaga
    29581 catatgtggg catttaccta cttctacagc tcctgaagca accttcatag agaaagccat
    29641 acaagttggg aatccacaat ccttacagtt tttctttggt gttaatttaa atatatctaa
    29701 agcttttaat gccattattt tttcccccta accttaacaa tttaattaag ctaatgcgtt
    29761 aaccaattct tttatagttt ctattgattt tggatgacga agtataactg cgtttgaacc
    29821 acctactaaa acacttgctg cagttgaaac ttccattgct atagttcttt cttctggaca
    29881 accccaatct ggctcatctt ctattggagc tatagcttct ttaacatgac ctacttcaaa
    29941 agctactggt gttatgatag gcatttgtaa tgtcttatca ttttgaccaa atgctgcaag
    30001 tctaattcta tccatagtag atgcaacgta ctcatatcca taaccaactg ctgaacatcc
    30061 aacattcata actatatttt cacctttaac gcctagttga gttaacaata cgtttaattg
    30121 tttagctagg tttatatcaa cagaagattc agctcctact ttatgtgcat aagccatacc
    30181 agctgatgct cctactcctt tgtagttatc ttctactgct gacataaata agcagttatg
    30241 tccatccaat gtttgagcta atttttcaaa taatttacca tctttttcat gatttcctga
    30301 accagctaca actaaaggta attttattgc ttcaattact gccttagcta catcagcaca
    30361 ttcatcaact gacttatcta atccatttgg gtcagaacca tcaaatttta aacaaataaa
    30421 atctgcttgt gtattagctt caacatattt agcccactct actggacaat tagctacatc
    30481 tttgtataat tccttatatg aatcagtcca gctttcagga taaacgtctg atatttgtat
    30541 acctattttt ggagaattac ctacttctcc atcaaaacta taaaaaggta atacattttc
    30601 cccacctaat ttaattgctt tttcccctat tcctacttca acttctgata tttttccaga
    30661 atatttttga gtagacattt taaatgccat atttttttcc cccttaagcc tattttaatt
    30721 ctaatttagc aaaaatttct ttcatagcca ctttagattt tgaatcttca ggtaaattaa
    30781 ctaaaggaat acctgtagaa tcatattcat atatcaattc atccataggt acaacaccga
    30841 ttagctctag gttatgcttt tcaatttctt ctttaacacc atcattcata accccatttg
    30901 gaactttatt tacaatcaaa agtattcttc caacacttaa ctttagttcc tcagctaaat
    30961 ctcttattct agcaacagct tgtatgctac gtcttgagca atcactaacc aataaaagtg
    31021 tatcaacatg tctagtgact tttctactta gatgttccat accagcttcg ttgtccatga
    31081 ctaagtattt gtaatgacca gatattttgt ttacttgttc tctaagtatc ccatttacaa
    31141 aacagtaaca tccttcacct tctgacctac ccattactaa taagtcatat ccttcgcctt
    31201 cttcgattat agaatttaat ctaaattgaa gatattgtgc ctttgtcata ccaccaggga
    31261 atgcattgcc taacttttct ctttgattta cttcttctct tatttctcct attgttgctt
    31321 caacttcgat acctaacact tcatttatgt tagcattggc atcagcatct acaactaata
    31381 ctggtccctt tttgtcctta accaaataat caatcaaaag ccctgtgaga cttgttttac
    31441 cagttccacc ttttcctgca acagctatat tgtatcccat tttaagactt cccccttaaa
    31501 tctagccaca gagccttatg tactctgtct gtctattata atttaggtac agaatgaaca
    31561 cattctccat gtacatcatg aagtgcttcc attagccctt cagataaact tggatgtgga
    31621 tgtattgcat cccctacttg ttctactgtt aatcctagat gaacagcaag tgacaactct
    31681 gtcaataaat ctgtagcatg aggtcctact actgctgcac ctattatctt atcagtttct
    31741 ttgtcagcta taacttttac aaatccttgg aaatgtccta tagcttgtgc ttttccaaga
    31801 cctctaaaat caaattgacc tacattgtat tctacacctt cagcttcaag ttgcttttca
    31861 gtcttaccaa caccagcaac ttcaggttca gtataaacac atcttggaat tgctctataa
    31921 tctactacct tagtcttacc taaagcattt tcaactgcta cgataccttc tttagaagca
    31981 acatgtgcta ggaaaggagt atctattata tcgcctattg cgtatatacc ttctacatta
    32041 gtttcaagat gttcattaac tactactttt cctctttcca tttcaattcc tatatcttct
    32101 actccagaat tgtcaaggtt aggtcttcta ccaacacata ctaatgcata ttgtgcttct
    32161 ataacctttc cattagaaag agttgcaaca gccttaccat caactacttc acaagtttgt
    32221 actccaatac ctgtaataac cttaatttta tctttcttaa attgtctaag tagttgttta
    32281 gctacatctt tgtcttcatt taatagtatt tggtcaacca tctcaactat agttacttct
    32341 gttcctaagg ctctaaagaa ctgccctatc tcacatccta taactccacc acctactatt
    32401 aacatagact caggtatctc ttcaagtcca agaacttcat cactagttat tacaactttt
    32461 ccatcatatg ggaacattct tggtacaact ggaacagaac catttgctag tattatctta
    32521 tctgctttta tagtttctac tgttccatca tctttagtta cttctatagt gtttttatca
    32581 attaattttc caaaaccatt aactaagttt actcctcttt tttcaaataa gaactctata
    32641 ccacttatta attggtttac aactttattt tttctttcca ttatggcagt gaagtttggc
    32701 ttaattgtac catctatttc tataccaaaa tcctttgctt cttttacagt attcaatact
    32761 cctgaagaag caagaagtgc tttagttggt atacatcctg catttaagca agttcctcca
    32821 actcttcttt tttcaacaac tgttacatct gcaccaagca tagaagcttt tatagctgct
    32881 acatatcctc ctggtccacc acctactact actatcttca tctttaattc ccccaaacct
    32941 aatataatct acaatcttgc aatactataa acctgcggca tccagtatag caggtatatt
    33001 ctcttctgcc ccaattgcca ttatatgaac accatcacat aaatcttctt cttttaattg
    33061 tttgataagt tcccctgcca tttttattcc ttcacttacc cagttttctt ttccagctgc
    33121 tcttaatctt tcgatttgtt catctggaac aaatattcct ggtacatttg cagtcataaa
    33181 tttagccatt cctggtgatt ttaaaggtac tattcctgct aataccttgc aatccatatc
    33241 ccttgttaat tctctaaact ttctcattgt atttatgtca taaagtgctt gtgtttggaa
    33301 aaatttagcc cctgctttta ttttcttttt catttttaat aattgaactt ctattggaga
    33361 atattctgga gttactgatg ctcctagata aaaatctgga gaacctttta acttattacc
    33421 agccatgtcc gtacctgcca ttaaagtctc agcagtttga agtatcccca cagaatctaa
    33481 atcaaacact cccttagctt gtggatggtc tcctacgcta gtatggtctc ctgttagtgc
    33541 caacagattg tttatcccaa atacacctgc tgacaacatt tctccttgta tagctattct
    33601 atttctatct ctccctgtta tttgaataac tggctctaat ccagcatctt ttaataattt
    33661 acatgtagca agagaagttg ctctcataac tgccgattga aagtctgtta catttgctgc
    33721 atgaactctt ccaactaacg gctttgcaca ttctattaaa tgtgaaaggt cagtcccttt
    33781 tggaggagcc atttcagtag taactgcaaa ttttccactt tctaatgttt ctcttaataa
    33841 gctcatctct aatccccctt ttaaccttct aaaaaaactt ttatgaattt gctgctgctt
    33901 ctttcttttt ctttgcactt aaacttcttg gattattttg cttagaatag tcttttggtg
    33961 gtcttatttc tgctaagtta tctaattgtc ctatagcttc tagtctttca tatatcttta
    34021 tccaagcaca atcattctct gagtttactt cacacttacc atttttagct ccaccacaag
    34081 ccccattcat aagaccttta gcacacatag ttactggaca tatcccacct gtccaaccaa
    34141 gttcacagtc tccacaagcc ttacaggctt cttcatattc tccaactctt tgtacttccc
    34201 ctataaacaa agtattgttg gctggataaa ctggcttatc ttttaaattc ttaactattg
    34261 tctgtgtacc atctccacat gctaaagata aaactgcatc agcttcctta gtctcttctt
    34321 ttagagcctt taaatccttt ttagacttta aaaggttaca agcagggtca agcattacat
    34381 atcctaagat ttttttgcct tcaccttcaa gagtttcctt catttttaaa acttcttctt
    34441 ccccaccact tttacaagtt gctgcacatt gattacaacc aactaaaacc agcttgtcaa
    34501 aatcttttaa gtaccctagc acttcttcta atggtttatt ttcagaaatt atcatatcta
    34561 cttcccccct aaaccttcca catatatata tctatttatt gttttgtaat ttgcaagcct
    34621 taactacatg ttttgcaagt atagatgttg taactgaacc aacccccgct ggaactggag
    34681 ttatcattga tactttgtct aaaactgcat ttgtatcaac atctccacat aaatttcctt
    34741 cttcatcaac atttatacca acgtctataa ctaccgctcc atctttaaca aaactttcat
    34801 caaccatctt agctctacca atagctgcta ttagcacgtc tgcctctgct gctacacctg
    34861 atagattttt agtttttgaa tgacatattg taacagttgc gtgttcactt aataaaagca
    34921 ttgatactgg ctttccaact accattgatc ttccaagaac tgttacctta cttcctttta
    34981 aatcaacatt atagtgtttt aatatctcaa ctactgctgt aggtgtacat ggtggaaatc
    35041 cacttttatc cccttccatt accttagcag agtttattgg actaaaacaa tctacgtctt
    35101 tttccggtgc aataacatat tttataactt cttcatttaa gtgttttgga agaggtctaa
    35161 aacataatat accattaaca tttttgtcat cattaactct ctttaataca tcaatatatt
    35221 cctcttgtgt tatatcctca gctaactcta acacttctgt tgttatacct atgttttgac
    35281 atctctttag tgctcctctt tcataagata aatcatcact tcttgcccca actctaacta
    35341 ttgttagttt aggatttata ccttctttaa ttagtaaatc gacttctttt attaattctt
    35401 cactaatctt atcagctaca ggttttcctt ttataatttg tcctttagtt gacattcctt
    35461 ccatacattc acaactcccc tacaagcaaa cttaaattaa tttattctat ataccacata
    35521 ttaataacat agtatctact ttcgtcattt aaaaattatt ttccaagagc tttttcaact
    35581 ttggcataaa cttcatcaca aatttttaca ccatcttcaa ctaacttatc tacttctgtt
    35641 tttaccttat taacatattc tttatcttgt atagaattta tatttataac tacatttaat
    35701 tgtccaccaa gtatagcagc tcttaaacac tgaactccta caccaacatc acttatagca
    35761 agtcttgaac ctttatcaac taaatcttca tgtaacttaa tagcttcata gcaaactctt
    35821 actatgttta atggtacttc acaagcaact tttaatgcct tttccattgt ttctgctttt
    35881 attctttttt cttcttctgt ttctttagga agcccgtaag cttttgataa tggtaagaaa
    35941 cattctgcat catcatctat catttttaat aaatcttttt ctaactttcc tgccttactt
    36001 aatatctctt ttacactttc ttcatactca gcatacttct tcttccctat tgtaaggtta
    36061 cataccatac tacctaaagc catacctatt gcacctacta gtgcagcagc tcccccacca
    36121 cctggtactg cttctttgga agatagtact tctacaaaat caacacaagt tttatctgct
    36181 attttcatga taatcctcct tgaaaggatg taggaaaata tctcaaattg ttcgagatat
    36241 tttacgaact attttaagat atctcctact ctttatttat aaattaattt ccttagaata
    36301 atccagctat tactccattt tcatcaacat ctattttttc tgctgctgga acttttggta
    36361 atcctggcat cttcattatt tctccagtaa gtgcaactat aaatccagct cctgctgaaa
    36421 ttgttgcttg tcttacagtt attctaaatc ctgttggtct acctaattta gtttggtcat
    36481 cagttaaaga atattgagtc ttagccatac atactggaac tttagtaaat ccaagttttt
    36541 ctaagttagc tatttcctta tcagcttcag gagtataatc tacaccatct gctccataaa
    36601 tcttagtagc tatagcattt atcttgtctt ttatagataa atcatcttca tagcagaatc
    36661 taaagttatt ttcttcttca tccaataatc ttaaaacttc tttagctact gcaattccac
    36721 cttctccacc ttttgcccat acttcagaaa gagcaacatt aacacctaat tccttacact
    36781 tgtcttcaac aagtttaagt tcagcttcag tatcaagtgg gaatctgttg attgcaacaa
    36841 ctgctggtaa tccatatact tgagttatgt tttcaacatg ttttaataag tttggaagtc
    36901 ctttttcaag agcttctagg ttttcgttat ttaagtctgc tttagcaact ccaccattgt
    36961 attttaatgc tcttacagta gcaactatta taacagcatc tggttttaag tttgccattc
    37021 tacattttat atctaagaat ttttctgctc caaggtcagc accgaaacca gcctcagtta
    37081 ttacataatc tgcaaagtgc atagccattc ttgtagctat aacagagtta catccatgag
    37141 ctatatttgc gaatggtcca ccgtgtacaa atgatggtgt tccttcaaga gtttgaacta
    37201 ggtttggttt taaagcatct tttaataatg cagccatagc accattagct tttaattgtc
    37261 tagctgttac aggttcacct tcgtagctgt atccaactat tatgtttcct aatctttctt
    37321 ttaaatcaga tatatcactt gccaaacaga acgctgccat tatttcagaa gcaacagtta
    37381 tatcgaatcc atcttgtcta actgctccat cacctttttt acccatacca tcaacgatat
    37441 ttctaagttg tctatcgttc atgtctacac atcttctcca agttattttc ttagggtcta
    37501 ttctaagtgc atttccttgg tgaatatggt tatcaagcat agcagctaaa agattgttag
    37561 cagctcctat agcgtgaaaa tctcctgtga agtgtaagtt tatatcttcc ataggaacaa
    37621 cttgtgcata tcctccacca gctgctccac ctttaacacc aaatactggt cctagagatg
    37681 gttctcttaa agcaactaat acatttttgt ctagtttagc aaatgcatca gcaacaccta
    37741 tagtagtagt agtttttcct tcacctgctg gagttgggtt tatagctgta gttagtatta
    37801 acttagcttt tttaccagtc tctcttttta gtagattgta gtcaactttt gctttgtatt
    37861 taccataaag ttctacatca tcttcaccta gtcctaattt ttttgcaacc tctctaatat
    37921 cttgaggctt agcttcttga gctatttcaa tgtcagattt aaatcccata actgaatctc
    37981 ctcccacaac ctaaatagat ttaaaatata atttttgtaa aaattgtatt aatatgtaaa
    38041 aattatattt tataagataa ttatacatgt aaaattactt aaaaactagc tactaagcta
    38101 ttaaaatatt caaacttaat agctagttat ttatctaatt acttacttat tacctatcta
    38161 ttgcctatgt ctaaccttta accaacttaa aatcccaaat caaaatttct atctatttta
    38221 tttatcctta agtgacatta atttatcttt tattgcttta atctgttctt tcgttgtttc
    38281 actagaatca tatggagatt gattagctct atcagaatca ataacatctt ggttataata
    38341 aataaatcct aaagattctc catcttctaa gttttggatt ataaattctt catcttcctt
    38401 atttctaatt ttatttccta ctacaaatac tttatttaca cctatatcat gtccaagttt
    38461 cttaactttt ctataagtct gtagacttct ctctcctggt tctacaacta ctataaaagc
    38521 atctacacct tgggctgttc ctcttcctaa gtgttcaatt cccgcttcca tatccataac
    38581 cactacatct ttattttgta gtattaaatg tgagcagagt ctcttcaaaa gaacatgctc
    38641 tggacataca catccagtac caccagaatc tacggttccc aatgtaagta atcttactcc
    38701 attgtattct ttacagaaat tttcagggat atcgtctact ttaggattca tcttaaacat
    38761 ttttccaaat gaacctacag aagcagctgt tctatctgaa actaattttt tcatttctga
    38821 tataggtact attgattcat atacttcttt tggaaaacct agtgctaaag ctaaattagc
    38881 atcagggtcg gcatcaacag caacaactct gtatccgtct tcagcaaaca tcctagagag
    38941 cattgaagaa aaagtcgttt tacctactcc acctttacct gttattgcta tcttcataat
    39001 ccctaccacc ttttattact atttgctttt tagtagttaa ttttacagtt aaatattttt
    39061 tatactctat atttttatac actaaaaata aactacagct agtccttaca ggaaccaatt
    39121 aaatttaccc tactagcttt tcaaagcagc atgtatagtt ttattattct ttaatattgt
    39181 ttatatttta tttattcagt atattcatta tattttgatg ttctttactt taatatttga
    39241 ctttattcaa ctttattcaa ccagaaactt cttataaaaa cgtttattct ataacttcac
    39301 tattttttaa gcttcagcct tttctgccat cttttcttca actaatgctt caaagtgaac
    39361 acgttttttc tctatatctt ctattattgt agctgctaac tcttttggat ttgtatttac
    39421 agtaaatgta gctccaactt tatcttttaa ttcactagtt aatatatcca ctgctcttga
    39481 agaaccagct attggaggca ttattcctaa ataagtgtct ataccagaag ctacaacata
    39541 gcatcctata gcaactgctt tttctgacat ccactctgga gctataccta caactggaag
    39601 gtcacacata tccatatcta agtaattagc tgctgctcca actaaatcta gtatacgact
    39661 tatatcaaca caagaaccca tatgtagtac tggtgggatt cctactaatt tacagacagt
    39721 tgcaagacct ttacctgcat atttttcagc cgcatcagat tccattaaac caaacttagc
    39781 tgctgctgaa gctccacaac ctgtagtaac tactattatg tcgtttttga ttaattcttt
    39841 cattattgtt acgtgagcct catttgaaac tcctttagca ttgttacatc caactacacc
    39901 agctgctcct cttaatactc cagattttaa acagtctgtt aatggcttaa ctgttcctgc
    39961 tgggtctata tgagagttta caactctgtc taattgattt actactgctt ctacactata
    40021 cccaactgtt gcgcctgatt ttaattcagg tataagtact tttgatttat ctctattttt
    40081 aaagtttaaa atagcttctt taactattgc tttagcatca tcaagtgctt gttcttctct
    40141 aaattcaata taagttgaac ctgttatctt agcttttgga gaagttgtta caaatttagt
    40201 atggtaacaa tctgcaactc tagctaatgc agggaatata cattgaacgt caactattac
    40261 agcttcaact gctccagtta ttattgcaag ttcttgttga tggaagtctc ctgctatttt
    40321 tacaccatgt ctcattgtaa tctcattacc tgtacaacac atacctgcta agtttattcc
    40381 atcagcacca acttctttag ctaaagctac taagtctggc tcttctgatg ctaaaactat
    40441 catttctgat aatgctggct catgtccatg taatattatg ttaactttat tttcttctaa
    40501 tactgctaaa ttcgcctctg ttcttcttgg cactggagtt ccaaataaga tatcgcttag
    40561 acgagtacct atcatagaac caccccaacc atcagccata gaagttctaa gactcatgtg
    40621 tattaaacta tctatatcac cagtacatcc tatatgagta gagtgcatta ttgttgcaat
    40681 ttctctatct atagctcttg gctctatagc gtattctttc catatttttt gtcttggttc
    40741 tggtgcattt tttataaatc ttaatgtccc aaaaggtttt ccaaattcca ttaatgcaat
    40801 ttctgccact tcatgtgcta catcatagat atctctacct tctgtctcta catcccattc
    40861 tttagctaaa gtaattaact tagcttcgtc tcttatttta tagtttccat ttggatcagc
    40921 taatgctaat gtatgtgcta tatctctagc atggtctgaa tgggcagctg tcccaccagc
    40981 aaccattctt gcaaagtttc tacctactat agtatgctca tctgctccac ataaccccct
    41041 tggagtctta gggcttattc tacatggtcc cattccacat attctacagc aaacaccttg
    41101 aagaccaaaa ccacattgtg ccttaaagcc ttcttttcta tcatacattg tttctacgcc
    41161 ttcttctcta gctttagcta tcatcttctg gctgtttaaa tcaattgtta acattttttc
    41221 atccatattc ctatcccctt tttttgattt ttaaaatccc ccaggtgact gacttcatgt
    41281 cataaaataa taaaataaat gctttctaag ttgattatat actaattttc agtgctttgc
    41341 tttagattta aatatttttt taacaaaatt agataataca taaaaatttt attttctaca
    41401 cttaatttaa gttgatttta tcgcttgttt ttggctattt gttaatatct aaacctactt
    41461 ttttgatact tcagtttaat taaattatgt tttgattttt ttaaatcaaa tttgaaattt
    41521 ttctagtcat ttttatatta ttgcagattg tttgtgtatt tattatcttt cactactgtt
    41581 tggacttatt acagattttt actccctaaa cttaattagt aaaaacttct tagaatagca
    41641 ttttttggac taattttttt gaattttttt atttttccta ctttaatatt aattatagct
    41701 tcaaattgaa catttgtata tattcatttg aagctataat tattttttat ataaaacttt
    41761 tttaatacag cattaattta gacattttga ctattttttc tactttaatt catttatttc
    41821 aagcgattta tcctatttca tatttttagc tacaatagcc ccatatgcaa tagaatataa
    41881 tttagattca tgtgaatcag ctcttgacac taaaactata ggagcttttg cacccataat
    41941 tatacctgct gatttagagt ctgccatata tgtaaaggct ttccctattc cattaccaac
    42001 ctctattgtt ggaaccaata tgatatctac atctccagat acttcacttt taaacccttt
    42061 tatctcacta gcttcttttg aaacagctaa atcaaaagcc agaggacctt ctacaattac
    42121 attttcacca aattctcctt ctttacaagc ttttgctaac atatctgcat ctacagttgc
    42181 ttgcatctta ggatttactt tctctttagc tgccaaacat gcaactttta cagtttccat
    42241 tcctaaagct tttgcagctt ttatagaatt ttttaatatc tttgcctttt gttcataatc
    42301 aggagctata ttcatacctc catctgtagt tataagtaat ttatggtatt tttctaactc
    42361 atataccata acatgactta ataaactatc agttctaaga ccatagtctt tatttaacac
    42421 ttcttttaat atgacagatg tatctaataa tcctttcatt acaaagtcgg cttctttact
    42481 tgatactaat ttaactgcta tttctgcaca ctctttgata tcatccttat ccactatttt
    42541 gataccactt aaatcaaatc ctatttcttt tgatatttct cttattttgc cttcatttcc
    42601 aaccaatata ggagtaatta tatttctttc tacagcatcc tttattgcca ttaaaacttc
    42661 tttatcatga gctgctgcta cagagagtac aattttctct cctcccttaa gttgttctaa
    42721 aatatcatct aattttttta tcaaaagtaa cccctccctt atatatattt aactaaataa
    42781 taccatattt gctatacctt ttctcaattt attttttatg ttataaagaa tttatataac
    42841 taaaagacag tttaatattt tattctggga tttcatcaat aactcttaaa agctttgcag
    42901 gaacaccacc tacaatagta ttttctgcaa cattttttgt tacaactgct ccagcagcga
    42961 taattgcatt atctccaata gtaactcctg gaactacagt tacattagca ccaatccata
    43021 cattttttcc tattgtgatt ggagaaggat aagttgtacc acgtttctct ggtgcaatac
    43081 catggttaag tgtagccaat accacattat gcccaatttg cgtattatct ccaatctcaa
    43141 tccccccctg gtcttgaaaa tggcaactgc tatttataaa aacatttttt cctattgtga
    43201 tgttttttcc acaatctgta taaaatggtg gaaacataaa aaatgattca tctacctttt
    43261 ttccaattaa ctcagaaaat aaactacgga cttcttctgg ttcatgataa gaagaattta
    43321 acttacttgt gattttcatt gcttcattac tcaacttaac cataaattca tgtatttctg
    43381 atttactaga aacaggtttt ctttgattga tatgtgttaa aaattcattg atattcattt
    43441 tatacatcct ttcaatatgt gctttaattt atttgtacat tctaaataat attttaataa
    43501 aaattaattt aatttatttt tattaaaata
//
LOCUS       DAMJVK010000027        42719 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.027, whole genome shotgun sequence.
ACCESSION   DAMJVK010000027 DAMJVK010000000
VERSION     DAMJVK010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 42719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42719
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.027"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            173..310
                     /locus_tag="PC414_001479"
     CDS             173..310
                     /locus_tag="PC414_001479"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463877.1"
                     /translation="MGNSDCSKMLAFYEIMKMLETIDEAKFYNCKEEIKSEFKHIKKE
                     D"
     gene            complement(338..730)
                     /locus_tag="PC414_001480"
     CDS             complement(338..730)
                     /locus_tag="PC414_001480"
                     /inference="COORDINATES: protein motif:HMM:NF024252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2463878.1"
                     /translation="MYKDESNIFSKRLREEREELGLMQKEMANKLSLPANTYNGYETG
                     KRSPALDVVRHIADTLDISTDYLLGRTNIKINISNITEKELIKKLNPSDDMKEILDIF
                     SELDDDSKNKALKIAKLFLEEQNTKNKE"
     gene            918..1112
                     /locus_tag="PC414_001481"
     CDS             918..1112
                     /locus_tag="PC414_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008422227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2463879.1"
                     /translation="MKNYILKSLRVRQGLRQRDLAKILGMNPSTYSSKENGDRRFTVS
                     EAIKISDFFNTDLRDIFLNK"
     gene            1148..1939
                     /locus_tag="PC414_001482"
     CDS             1148..1939
                     /locus_tag="PC414_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HDF2463880.1"
                     /translation="MNNLQVIERNNKRVLTTQQLADVYETDARNISNNFNNNKDRFIE
                     GKHYFCLQGDDLRIFKRGSYDIGIAPNVNKLYLWTERGANRHCKILDTDKAWEQFDNL
                     EETYFKVKQQKLTCIEDVLIESLKEMKDLRLQVNQANNIALEAKTEVKTIKEVVSLNA
                     TDWRKDTQQLIARIAKKQGGFEHINMLRRESYELLNKRFGVDLHRRLINKRRKMAEEG
                     VSESKREKVNNLDVIQDDKKLIEGYVAIVKDMALKYGISSDLSKN"
     gene            2001..2168
                     /locus_tag="PC414_001483"
     CDS             2001..2168
                     /locus_tag="PC414_001483"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463881.1"
                     /translation="MENKKIEEIKNALIGVNQIEWSLVKSFIDMYFSKKAAKLEIDDL
                     DNFDLYIKRKF"
     gene            complement(2165..2422)
                     /locus_tag="PC414_001484"
     CDS             complement(2165..2422)
                     /locus_tag="PC414_001484"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463882.1"
                     /translation="MKVGDIMTKIYACLAGNWVNLCDDPECKMGSNHSSPYIWWEEGA
                     DVWSPSNKEEEHTMYQQDYVHIYYKGVDYRIHPIFIQIASN"
     gene            2480..2677
                     /locus_tag="PC414_001485"
     CDS             2480..2677
                     /locus_tag="PC414_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2463883.1"
                     /translation="MLIGDNISQILRKRNIKPYKLAKELGIDESGLYKIIRNEKKNPT
                     INTLIKIADYLDVTLDELVGR"
     gene            2716..2925
                     /locus_tag="PC414_001486"
     CDS             2716..2925
                     /locus_tag="PC414_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463884.1"
                     /translation="MAKAVAKEPLFYRAKDVAKFLDICEATAYKIIAELNEELEKEGF
                     KTFSGRVSVAYFKERYCYKPRKGVI"
     gene            2926..3066
                     /locus_tag="PC414_001487"
     CDS             2926..3066
                     /locus_tag="PC414_001487"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463885.1"
                     /translation="MNVRVLIAYIQFCNDKQIKASFEGLRKYNRDIELVNLYLPAHLK
                     IK"
     gene            3098..3238
                     /locus_tag="PC414_001488"
     CDS             3098..3238
                     /locus_tag="PC414_001488"
                     /inference="COORDINATES: protein motif:HMM:NF015807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HDF2463886.1"
                     /translation="MQREQTTIRLPKELKEKLEKQASKKGRSFNSILLSILQEFIQNP
                     NV"
     gene            complement(3216..3404)
                     /locus_tag="PC414_001489"
     CDS             complement(3216..3404)
                     /locus_tag="PC414_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HDF2463887.1"
                     /translation="MAKMGTYKNPHFAIRIPKEKLDKLKYIAEYNARSANKEIEFLVT
                     KHIEMFEKEHGPIHLDSE"
     gene            3560..3718
                     /locus_tag="PC414_001490"
     CDS             3560..3718
                     /locus_tag="PC414_001490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463888.1"
                     /translation="MLANRVRTGLRIPYDLNTKLILIAEERGMSKNSLILQILWDYIK
                     EIENKEVK"
     gene            3718..3945
                     /locus_tag="PC414_001491"
     CDS             3718..3945
                     /locus_tag="PC414_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463889.1"
                     /translation="MVELVKEFDLQTIKVGNAVKVNCKRFGFEIDCIVVVATEKELNL
                     AYFDEGRGCMEYQALITEDIQDGDYEIKILS"
     gene            4140..5030
                     /locus_tag="PC414_001492"
     CDS             4140..5030
                     /locus_tag="PC414_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HDF2463890.1"
                     /translation="MDWLKNRQLGIGGSDASAVAGLNPWKTSVQVYIEKKEEIPIETK
                     SFRMELGNRLEGLVAELFTEETGLKVRNVNGMLKNEKYPFAIANIDRAIVGEKAFLEC
                     KTTNSFSIKEWENGVPLHYEIQCLHYMAVTGATHCYIAALLGNEKFVWHKINRDNEVI
                     KNLMKIESEFWEENVLKDILPIPDGSDAYSEFLKTRYKNSVKEKIELNLLEDGISKLK
                     RYDDIVLQMKELKGEKQLIEQEIQSEMREFELATLGGRIITWKGATKRSIDTKRLREE
                     MPDIAEKYTNISSYRTFKIK"
     gene            5043..5876
                     /locus_tag="PC414_001493"
     CDS             5043..5876
                     /locus_tag="PC414_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HDF2463891.1"
                     /translation="MASEKAKGALEKKVSGANTVKVSPSKGMEQLMNKMASQIKKALP
                     SMVSSERFQRVALTAFSNNPRLQSCEPMSFIAAMMESAQLGLEPNTPLGQAYLIPYGN
                     KVQFQIGYKGLLELAQRSGKIKTIYAHKIRENDKFEIKYGLHQDLVHEPKLNGDRGEI
                     IGYYAVYHLDTGGHSFSFMTKEEIIEFAKSKSKSYSSGPWQTDFDSMAKKTVIKQLLK
                     YAPLSIELQKAMVGDETIKSEIDEDMSMVVDESESLEVDFEVKENMDGKVSVEEAINV
                     D"
     gene            5891..6829
                     /locus_tag="PC414_001494"
     CDS             5891..6829
                     /locus_tag="PC414_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2463892.1"
                     /translation="MNEDKSVIEKLNILSGGYGLMPRIIARDRWLTVGARMLYSYLTS
                     FAGNDGTCFPSRDLICYELDISKDTFTKYKKELEMSGYIRVHKNKSKQGKMQNNIYEI
                     VFDRTYIDECISKRGLKEEKKKKKPCTKKQDTEPYPKNVDMEPCPTFPDTTQPDTENM
                     DTNSNSINSNSINNMYIVKQPVDNFLKEFKKLYEENIGVIYPVTAEWLLEVSNEVDIR
                     VFKRAIEICAEKMNMNLAYLKGILKKWKDANITTYEQLESYRLQQENKKTKKVVNNHV
                     SKNKFANFEQTFTKYSENELDDIIKKSQKEKFGVGS"
     gene            6849..7247
                     /locus_tag="PC414_001495"
     CDS             6849..7247
                     /locus_tag="PC414_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463893.1"
                     /translation="MELKFREWNKNGKEMYSYDEMVCYSKNLLREWVYSGVYLPTSNE
                     NFEVMIYTGLKDCIRKEIYEGDIVSYILSFEEFIGEVKFEEGFFVIDNEVLGECVGLF
                     HEIAVVKVIGNIYENPEMLEKIRKPKVLEV"
     gene            7250..7522
                     /locus_tag="PC414_001496"
     CDS             7250..7522
                     /locus_tag="PC414_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463894.1"
                     /translation="MKHECKKLFLECDKGSFEINDTRIGEVMFESTEIDNPFERVKYE
                     GKATFEIVSGWEYLQREMLWLKILHLSAVVAKIMQYKMLGILIREM"
     gene            7527..7907
                     /locus_tag="PC414_001497"
     CDS             7527..7907
                     /locus_tag="PC414_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463895.1"
                     /translation="MAKIWMDAGTFLEKTIDIEDMFELNLRAIRKRNEKIKNEIEKQI
                     KYEDSEIKTTNRGGPGKIIKIFNINTGEVKILKSAEEASRYIKVSRSYASYLARENKS
                     TEDGWKAEYIQGATDGISKCGTSN"
     gene            8132..8308
                     /locus_tag="PC414_001498"
     CDS             8132..8308
                     /locus_tag="PC414_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463896.1"
                     /translation="MDDRLEIINASVNYIQMICESSNIAIIAERGRVRILDLETKEKY
                     DLLKNKLEEMLEEI"
     gene            8422..9111
                     /locus_tag="PC414_001499"
     CDS             8422..9111
                     /locus_tag="PC414_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463897.1"
                     /translation="MDLNKIMNDALVELEENGFVEEVVKKQLAETIKRVVNEVFGNWG
                     DFSKKIEKHLSENININLDKLDIQKYNVLVATTVKEKIDMTMKVEAIEHLKRNLDNML
                     VGAEKEYKMSELLEILKMDKYDIDEYEGYEDCITFILDENKYGSGWIEIDKEPNKSKY
                     RCEHSILLRKDGTIASYRYENREISSKDIMSGFDNFGDLLFKIFAHGSKIILDLGNDI
                     EDYDLTNGEDY"
     gene            9129..9350
                     /locus_tag="PC414_001500"
     CDS             9129..9350
                     /locus_tag="PC414_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463898.1"
                     /translation="MSKYVLRWQKGLLLDERKINYSCGSIENLKKKAELLAKDDKILL
                     VTIDKVEEVIKDTRSQKMAEYFEDEGVEV"
     gene            9347..10351
                     /locus_tag="PC414_001501"
     CDS             9347..10351
                     /locus_tag="PC414_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HDF2463899.1"
                     /translation="MIIHKFIIHVLDKNSDTPILNDFEGRVSQDIEAFFQKKISKVSR
                     DNDIRTAVFNDYSNNLIKKCCEQIIYDESSFLNNSKEIAAYLFDVMKLNATLESCDLA
                     ICLYSQKDEKKVAILKLDYNNSYTHSIEFKDDKFNIQMSKNEINIQETKTVKIAALVG
                     LSGMNDKYHLRVLDKDAEKEEANSKFVTEFLNATKIKDDKYKTKKFKNTAENWITNAL
                     SNDIKQAEDVRSILNYTLREKHEIDINDFVDKTIKDDKLKDSFKEHMEEKGLVEGFSI
                     DKKWVDKKLKKRNIKTDNGFEIKGNLTDFEDPMKYTVRQNQNGSIDIVIKNVTFYEEK
                     "
     gene            10433..10762
                     /locus_tag="PC414_001502"
     CDS             10433..10762
                     /locus_tag="PC414_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463900.1"
                     /translation="MKIFLLTILLIIICILANYVKNRIYKKSINNLKYKYSVGEKIIY
                     HQINCYYNRMVGCEILEKCYSTKFRKRNTPLYKVKAYVGDNDTTWVIPEWRIECLATT
                     YGEFPKY"
     gene            10799..11170
                     /locus_tag="PC414_001503"
     CDS             10799..11170
                     /locus_tag="PC414_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463901.1"
                     /translation="MFNIYKVKIKTKRTLEQVRNQSVDFEYSEKGLKNTLKYYNLIDD
                     LKVIVVKFGDEYCLANYNEEDRKIIMEAHYLLEQDEYTGCYINEYERFKKDWENGNCD
                     GEACMVFSDDEIEIIEKLREG"
     gene            11173..11355
                     /locus_tag="PC414_001504"
     CDS             11173..11355
                     /locus_tag="PC414_001504"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463902.1"
                     /translation="MNKRIKMKKRLIHKKCDERCVNYDFVISNNLITCNVCIGCKYKE
                     NMDKVCEENYKKLRSK"
     gene            11430..11771
                     /locus_tag="PC414_001505"
     CDS             11430..11771
                     /locus_tag="PC414_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HDF2463903.1"
                     /translation="MANIYCENYNCKNYFEDMCMLERIEINNFKECESYLEGKNELYE
                     LENGYTIHPKDLKMVKSKDYSVEVTHIPTGITVKCRSTNSILKNKNKCLEVLEEELTK
                     INSHLELEDLR"
     gene            11914..12312
                     /locus_tag="PC414_001506"
     CDS             11914..12312
                     /locus_tag="PC414_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463904.1"
                     /translation="MQKDVWLYSWDDECFGSDEYESKEEAIQGAKEELRRFGEVSRLV
                     YVGQKEEVNIPNLNVEDALEHVQDRIDNEFMGYGEDWFEKIRVEDILILENRISKVFK
                     KWIDEFGYKPYWFVVRDTEEIELNEVANES"
     gene            12360..13085
                     /locus_tag="PC414_001507"
     CDS             12360..13085
                     /locus_tag="PC414_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HDF2463905.1"
                     /translation="MNEIMTNENLRVVADDLVTVYETDTGEKIVFARELHNNLEVKRQ
                     FIDWIEDRIKQYGFKENEDYSVFHKNMKNSTGGRPSKEYVLKLDVAKELAMVQNNNKG
                     REIRRYFIRLEKLLNRTLSNSQLSQINTIVNESLLKMEVKHNIQIEQLKKECSEYYRP
                     TSKTKYDISSYIKERLGISKVNEEFELVKKRTLLVLGADKWEDIPKDVLLNSLNLIDE
                     SIRIIKSERKTNQISFFEKDNFC"
     gene            13179..13667
                     /locus_tag="PC414_001508"
     CDS             13179..13667
                     /locus_tag="PC414_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463906.1"
                     /translation="MDNNINKKELFKKVEGRLHHYKFLSAEIKNLELDIESRENEIFG
                     CKAVGYDEKVSPTYAFNSSVENEIIKKERDITRLKKLKKDKEIEKKKIENALTCLDIR
                     EEHFFKLFYNSRMKNSMVYISLEMNSDRKICRCVRERLVYKIMDMLYPRIKENELPLF
                     KN"
     gene            14411..14584
                     /locus_tag="PC414_001509"
     CDS             14411..14584
                     /locus_tag="PC414_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463907.1"
                     /translation="MATKSILKNVDVRKKAFGRNLVSALENAKNKQEKEVVLSKKCSE
                     VPKDKIKDIFGRF"
     gene            14584..15195
                     /locus_tag="PC414_001510"
     CDS             14584..15195
                     /locus_tag="PC414_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDF2463908.1"
                     /translation="MSGYLIVNLSNMLGELEEEEVKKILSSFSCPLNKDVEDFLKNKA
                     IEFSKQGLASTHLVLTSYKGKPVIVGYFTLANKYFTIKRKTLTNSLARKVVKFGQYNE
                     ELRRYIIGAPLIGQIGKNYSNNYNKLIKGDELLKIACDKIKAVQLDMGGKIVYLECED
                     KPKLIEFYKDNGFVDFGKRSLDKDETDSLDGDYLVQMLKYLKK"
     gene            15272..15898
                     /locus_tag="PC414_001511"
     CDS             15272..15898
                     /locus_tag="PC414_001511"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463909.1"
                     /translation="MKKITKLILCSLLVISIASPLQVEAKTTHHSSSHVSIHKSSTKS
                     KSSRPKITKTKKATKTYRTNKSIKKKIKLKKQVNKKTQVPNKQAARKDKKTTTYNYYN
                     SYNSNRYYTTSSSGLSFWDCYMLGHLFNNNNKVSEQDIARELQKQGYSKQETDNILRK
                     ANQNQAKKEEKKKRVMMLIFSVLGALFIIGLGLIIFIKRESKGCNKSM"
     gene            15971..16660
                     /locus_tag="PC414_001512"
     CDS             15971..16660
                     /locus_tag="PC414_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2463910.1"
                     /translation="MNEKADLAHEDYLKGLKYKEIAEKHNVSLSTVKSWATRYWKQKG
                     CNQSKKVATKKRGAPIGNKNATGPPGNKNAEKFGFFSKYLPEETRELMQEISIKDKFD
                     ILWEQITIQYAAIIRAQKIMYVKDKEEMIKELKKHESTENGEKIEYEFQFAWDRQASF
                     LNAQSRAMSELRSLIKQYDEMIHKDWNLATEEQKTRVEKLKCEVDNLSKDDIGDDELK
                     ISVDYGDRNDS"
     gene            16638..17891
                     /locus_tag="PC414_001513"
     CDS             16638..17891
                     /locus_tag="PC414_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HDF2463911.1"
                     /translation="MVIEMIVRVNFNPDFKEANFTKKRYRAMKGSAGSGKSVNVAQDY
                     ILKLGDKKYQGANLLVVRKSEATHKYSTYAELTGAINRIYGKQADKYWKTTLNPLEIK
                     SKVTGNSIIFRGVNDAKQREKLKSINFSKGKLTWVWCEEATELMESDIDILDDRLRGI
                     LTNPNLYYQMTFTFNPVSSTHWIKRKYFDYKNDDIFTHHSTYLQNRFIDEAYYRRMQM
                     RKEQDPEGYKVYGLGEWGETGGAILKNYVIHEFPTEFEYFDNMRLSQDFGFNHANVVL
                     RIGFKDGELYICNEIYVHEMDTSEIIKIANSKGLEKNIFMYCDSAEPDRIKMWKSAGY
                     KAKGVKKGPGSVKAQIDYLKQLRIHVHPSCTNTIKEIQQWKWKQDERTGLYLDEPVEF
                     MDDAMAALRYSIDNKLKNNGISFLK"
     gene            17906..19336
                     /locus_tag="PC414_001514"
     CDS             17906..19336
                     /locus_tag="PC414_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDF2463912.1"
                     /translation="MYISETDLIKVQLKKESTFNLVKVIEHYILKHRPEKYKQGEEYY
                     YGNADINNKRRYYLLDGAKVDDFTKVNNKAINNYHKLLVDQKVGYSVGNPIVFNADDD
                     NLTKLLNDLLGEEFDDTITELYLNASNKGVEWLHPYINRKGEFKYVIIPAEEAIPIWD
                     SKRQRELVAFIRFYYIEDIDGNKIKRVEYYTENDVTYFIERGNSFIQEFLYDEYGKMT
                     DIQEGHFRINNKEQGWGKVPFIPFKNNEKCVSDLTFYKSLIDIYDNNISTLADNLDEI
                     QEVIYVLKEYPGTSLQEFIDNIRYYKSIKVDGGGGVDKLEINIPVEAKKELLDRLEKN
                     IIIFGQGVNPESQNTGDKSGVALKFLYSLLDLKCSKTEKKFKKAIRELLWFVCEYLKI
                     SGNKSYDYKTVQITFNHSMIINEAEKIDMAAKSTGIVSDETIVSNHPWVEDVNDELER
                     LKKQEDTQKEYDDLIPNNQDGVIDET"
     gene            19326..20687
                     /locus_tag="PC414_001515"
     CDS             19326..20687
                     /locus_tag="PC414_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013657754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HDF2463913.1"
                     /translation="MKHKDYWRKRFEQLEEAQNNKSIKYYLELEKQYKLAMSNIEKDI
                     LIWYNRFTENEGISLLEAKKLLNTRELEEFKWSVEEYIKYGKENAINQKWMKELENAS
                     ARVHITRLEALKLQIQQQVEVLYGNELDGIDKLMRDIYTSGYYHTAFNVQQGVNVGWS
                     LMSLDTNRINKVISKPWTSDGLNFSERIWGKHRPALINELHTKLTQSIIRGENPKVLV
                     NEFSKKFNVSKSQAKNLIMTESAFFASASRKDCFNDLDVEKYEIIATLDLKTSNICRE
                     LDGKVFDMKDYQVGITAPPFHCHCRTTTAPWFEDEEGYRAARDENGKTYYVPSGMKYK
                     EWYEKTFITDKKDDILSNKSWLKADFPTEKKFKKHVEKHLNEYGNITAEEYLSTARNL
                     LSEQLSNDVEGFLSKDNFLFKYRKSTNDFAVGRADGKISTLFKPKEGYNYWLEQIKEY
                     KEV"
     gene            20690..20851
                     /locus_tag="PC414_001516"
     CDS             20690..20851
                     /locus_tag="PC414_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003495392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463914.1"
                     /translation="MNCPVCGKDVDVFDICDNCNWQNSGPKETNYKGPNKMSLKEAKE
                     AYEKGKKVI"
     gene            21183..21575
                     /locus_tag="PC414_001517"
     CDS             21183..21575
                     /locus_tag="PC414_001517"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463915.1"
                     /translation="MAKFKKKSEEVEAFKWILGSPNTPIWFYQAFEKGTIWLDESLNS
                     VNRKGEVKKTICIKYKNGVIRATNGDWIIKDSEGKIYSCTCDEFEKYYKDLDNETKIS
                     NGGLECLREIALKDVNFKFELTGIKEEK"
     gene            21576..21773
                     /locus_tag="PC414_001518"
     CDS             21576..21773
                     /locus_tag="PC414_001518"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463916.1"
                     /translation="MFYLLLQVISICLSITVFMYVTGNIVFKIYLKSLRKEYESNIHE
                     VSDEILKRIRKELNKSLDESL"
     gene            21816..22046
                     /locus_tag="PC414_001519"
     CDS             21816..22046
                     /locus_tag="PC414_001519"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463917.1"
                     /translation="MKEYMIWFKSGNSISGIVDEDVADKLMQDFMEADSDCKDLKVYL
                     DEDGTTVIDLSQIEAISINNCNENNNIGFSKS"
     gene            22091..22654
                     /locus_tag="PC414_001520"
     CDS             22091..22654
                     /locus_tag="PC414_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HDF2463918.1"
                     /translation="MDWLKELLEGIKVEDNKIDVASLQKSIEKKIKETTITKEDYTNL
                     ETQLNTANETIKKFEGGMTKEDVENLKTTYETDKKTLEETYKKEIEEKDFNYWLNDAF
                     KSIKCRDEIALKAHLDIEALRNSKDRQKAFEEQINPLKQDKDYLFNATLEGEEPKIDT
                     ITPGQEPKINDFGFNFTGVRPHENNNK"
     gene            22666..23565
                     /locus_tag="PC414_001521"
     CDS             22666..23565
                     /locus_tag="PC414_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HDF2463919.1"
                     /translation="MAALNYAKEYSNVLAQAYPYTLNFGDLYATPNNGRYRWTGSKTI
                     EIPTISTTGRVDSNRDTIAVAQRNYDNAWEPKVLTNQRKWSTLVHPADINQTNYVASI
                     GNITKVYNEEQKFPEMDAYCISKIYADWTALGNTADTTVLTTTNVLEVFDKLMEKMTE
                     ARVPENGRILYVTPVVNTLIKNAKEIQRTVNIKDGGTSLNRQTTDIDTVKIIKVPSNL
                     MKTAYDFTTGWKVGAGAKQIFMSLVHPSAIITPVSYQFSKLDEPTAVTEGKYFYFEES
                     FEDVFILNKKADAIQFVVEGAGA"
     gene            23565..23798
                     /locus_tag="PC414_001522"
     CDS             23565..23798
                     /locus_tag="PC414_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463920.1"
                     /translation="MAQVRKLNRILTIEECKIDDFLEMGYDLIDETGKAVKYGKSLNV
                     KDLIAENNILRSKVESLEEENKQLKEKNKLTKK"
     gene            23808..24209
                     /locus_tag="PC414_001523"
     CDS             23808..24209
                     /locus_tag="PC414_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463921.1"
                     /translation="MENNMIDDIERRLQGFGYVLKDGDKWLIDFVREKIENIIKLDCN
                     ISEIPKELHNIEIDMIAGEFLFTKKNMGQLDIESINFEAVEKSISEGDTKVDFAIGSG
                     SQTPEQRFDSLVAYLTTYGKNNILTFRCLRW"
     gene            24203..24550
                     /locus_tag="PC414_001524"
     CDS             24203..24550
                     /locus_tag="PC414_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463922.1"
                     /translation="MVSKTRKAIEMLYRDKCTIVEYQPIKDPVTKRTNNKEVIVLENQ
                     PCKLSYKNIVSATEGKVAKLEQTIKLFISPDIEIKAGSKLIINDKEYVRSGESAIYPN
                     HQEIILELFKDKA"
     gene            24550..24975
                     /locus_tag="PC414_001525"
     CDS             24550..24975
                     /locus_tag="PC414_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDF2463923.1"
                     /translation="MARWGSVDFREFKRVCKKMEKLTKIDLDKFCKDAARELAARLLG
                     KVIRRTPVDTGFLRQGWNGVAYARSLPVYKQGNNYIIEVVNPTEYASYVNFGHRTKDG
                     KGWVKGQHFLTISEMELQSQVDKIIEKKLLILLKGVFDA"
     gene            24968..25405
                     /locus_tag="PC414_001526"
     CDS             24968..25405
                     /locus_tag="PC414_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463924.1"
                     /translation="MLNNIIDGISVKLDKSFGEKYTIYSEDVEQGINEPCFFIVPLNP
                     SKTPYPSGRELKKNSFDVHYFPRSEAKNFEINEIAEMLLEELEYIEIDGDLVRGTNMN
                     FEIIDNVLHFFVDYNYFTIKNNDTNKMDTVELFGGLKRGDNFE"
     gene            25398..25574
                     /locus_tag="PC414_001527"
     CDS             25398..25574
                     /locus_tag="PC414_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463925.1"
                     /translation="MNKTLSKEDDYKFTKEQIVNSKKYVNRKDLLNAILKENELYSFS
                     EVEEIINKFMKGVS"
     gene            25575..26885
                     /locus_tag="PC414_001528"
     CDS             25575..26885
                     /locus_tag="PC414_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HDF2463926.1"
                     /translation="MALGGGTFVTQNKILPGSYINFISAKRATSSLSDRGIVAIPLEL
                     DWGIDEDVFQVTSDDFEKYSVKYFGYDYTHEKLKGLRDLFKNIRLGYFYKLNKGVKAS
                     CTIATAKYSGIRGNDLKVTVTTNIDDNAKFDVVTLLDNKKVDTQIAKVITDLQDNDYI
                     TWKKDATLEASAGLVFTGGTNGEAVTGAEYQAFLDKIESYSFNALGCLATTTEIKSLF
                     VEFTKRMRDKVGAKFQTVLYKKSDADYEGVVSVENKIKDKDLVESSLIYWATGAIAGC
                     DINKSNTNKKYDGEFDVGVNYTQIQLEEALKSGKFIFHKVGDEVHVLEDINTFVSFTD
                     DKNDDFSSNQSVRVLDQIANDIATLFNTKYLGEVPNDKSGRISFWNDVVKHHEQLQNM
                     RAIEDFKADDVSVEPGSDKKTVVVSDAVKVISAMSKLYMTVSVS"
     gene            26907..27377
                     /locus_tag="PC414_001529"
     CDS             26907..27377
                     /locus_tag="PC414_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HDF2463927.1"
                     /translation="MAQQIKARDTISASKAECFVTIKGKRYNFMQAINLEAKMEKNKS
                     EIPILGSTTKGNKSTGSKYSGNATFYYNTSIFRELLYEYKETGEDIYFDIQITNEDPT
                     SSVGRQTIILEDCNMDSGIIAKFDADGEYLDEDMDFTFENWKLVEKFNIANGME"
     gene            27430..27687
                     /locus_tag="PC414_001530"
     CDS             27430..27687
                     /locus_tag="PC414_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463928.1"
                     /translation="MIKIKDKYEIKDSISFDYSNKRPLEERVSEMYKKAGKYLIDISD
                     KLATDTIDGSSLKSIIIKFEINEAGVATIEKQTKYLVMEVE"
     gene            27692..28132
                     /locus_tag="PC414_001531"
     CDS             27692..28132
                     /locus_tag="PC414_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDF2463929.1"
                     /translation="MGDLNAFLSQNAIKVENRKYVASERFIGEDGKAIEWELKAIDSD
                     RDRQLRKDSTIRVPILNKKGKATGQYTSETDFNTYTLKLCVETIVFPDLHDAELQNSY
                     GVMGAEELLTTMLTPGEYTDLSSEVGEVNGFDRTFEDKVEEAKN"
     gene            28455..28613
                     /locus_tag="PC414_001532"
     CDS             28455..28613
                     /locus_tag="PC414_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463930.1"
                     /translation="MDNFLQNVLERLSVSLIVFLVSNLLKKRKKPLKAGTKSGWEFDL
                     KIRFHKFK"
     gene            complement(29322..29531)
                     /locus_tag="PC414_001533"
     CDS             complement(29322..29531)
                     /locus_tag="PC414_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463931.1"
                     /translation="MKEYEAELNISISIPFFTVISYLVIVSIVMLCLSALNNFANISS
                     VPVSALGSTTGFIIFNLIISLFKNK"
     repeat_region   29822..30110
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=29823..29851
                     /rpt_unit_seq="gttttagattaactatatggaatgtaaat"
     gene            30270..30440
                     /locus_tag="PC414_001534"
     CDS             30270..30440
                     /locus_tag="PC414_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006862428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HDF2463932.1"
                     /translation="MSPKKIGRPVVGSPKTNDIKVRVDDETNEKLDEYCKKNNLTKAE
                     AIRQGIHLLLEK"
     gene            30566..31372
                     /locus_tag="PC414_001535"
     CDS             30566..31372
                     /locus_tag="PC414_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
                     /protein_id="HDF2463933.1"
                     /translation="MMNDLQIFKSEDFGEIRTVDIDNEIWFVGKDIAEALGYSNTREA
                     LKTHIDNDDIAEVVIHDGSQNRNMKITNESGLYSLIFGSKLETAKNFKNWVTKEVLPA
                     IRQHGAYITNNADPQALREKANEIESLDTVNKTIEILTPFLDNAGIDEKAKLLTAKTI
                     YKKAGIELPLEIEEKEHFFDTKQIATKLNMYSKSNKPAFHAIGEIIKKLDIQDNEKLV
                     VLESKRGWSGSVNKYSQSVIDKIRHWIGENNKPAKIQGEKKNFHVVYKIE"
     gene            31429..31605
                     /locus_tag="PC414_001536"
     CDS             31429..31605
                     /locus_tag="PC414_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463934.1"
                     /translation="MIEDLLNEYNLKTDEDVEYFVKFANALYELKQNSEEKFQEYAEI
                     LRGILREQEERKNK"
     gene            32026..32220
                     /locus_tag="PC414_001537"
     CDS             32026..32220
                     /locus_tag="PC414_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463935.1"
                     /translation="MWCVKELVEYKNLKSEFSIILTKWYVNSNIKDFTSLPSIGFILT
                     MWYVKKELLRRMLLAGEILY"
     gene            32624..33406
                     /locus_tag="PC414_001538"
     CDS             32624..33406
                     /locus_tag="PC414_001538"
                     /inference="COORDINATES: protein motif:HMM:NF021378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HDF2463936.1"
                     /translation="MGLFGGKEPCSICGGKGKNKVLENEYLCNNCFINFTIFSRERLK
                     ATSAMQVLADHEGIRKFINFSKTNRELLAKFVETNRINKFVSIDEDNNFIKISDIRKG
                     GDVIENVYAIDEIMQFELLEDGESIVSGGLGRAVVGGALFGATGAIVGGITGKKTTRK
                     VVDTFKIKITFNNINNPLEYVNLINTKTKTNSSIYQNACNEAQEILSVLSIIIKNNDK
                     TDNTQSKTSDSIEQVKGLKELLDLGAITEDEFNTKKKELLNL"
     gene            33473..35830
                     /locus_tag="PC414_001539"
     CDS             33473..35830
                     /locus_tag="PC414_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HDF2463937.1"
                     /translation="MATIQTSIRIFDGMTPAFRNMTNSINTTINSLERLQGRLNNPLN
                     TGGIQASQQSLNNIENILTRIEQNIGKADEQQRRFNEDINKGVSNTDRLLGSVKKITG
                     AYMSLKTLGGLGSLSDQMTNTNARLSLINDGQLSDGGLNKMIFQSAERSRASYLDTAQ
                     IVSRIGMNAGSAFSSTREIVSFAEQLNKKFIIAGASTQEMSSALLQLTQGLGSGVLRG
                     EELNAVFESAPNIIKSIADYLDVDIGKIRGMASEGMLTADIVKNSLLAASAETNAQFE
                     KMPLTIGQIFTSIKNNAVMIFGAIQKKIEDTVSSGGFRTFIVNVTDSLYVLGAVGYSV
                     FNGFIDLLSSPTFQGFANMMIVGISLIAQGLGWIITQALSVANVFTQNWSIIAPIVYG
                     IVGAFIAYKVVIYSVFLAQAIYTAITFTQCFVTALFAGELYVATKAMLIHQLMTLGVS
                     RANVTLCATMIMVVSAVALVIAAIFVGVAIFNHFAKTGISATGVVIGAFYFLGACIYD
                     VFAGAWNIVMAFTEFFVNSFNICIYGVQMLFYNLANFVINSMGDVGGSFDRCATTLAN
                     VFINGVNLAIKAVNGLIGLINKIPGVNIDTFGHGYTKNIDSVVGSLKNYQKTLKEPIK
                     PQDWKAPYMDIKNPFDSFNKGYKVGQNLENKLKDAFDINKIAEKAKKDLGLDDLWDKK
                     YGLGDGLGSAGLNSPLSDAAKGAKDTAGNTAKMAKTMDKSQEDLKYLRDIAEQEVINK
                     YTGVNIKIDMNNTNNISKDTDLDGIVNVLTEKLNDAMVVSAEGIV"
     gene            35846..36541
                     /locus_tag="PC414_001540"
     CDS             35846..36541
                     /locus_tag="PC414_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDF2463938.1"
                     /translation="MAYDFYLDGVQLPITPGKLEIKVTNKNKTVDLINTGEVNILKKE
                     GLSEISFEAEFTHNKLPFCRGQFRDVQFFLSKLELLKTDCKPFQFIVSRELGNKVLFN
                     TNMKVSLEEYNIVEDAENASDTKVAIKLKQYRDYSTKKLVPAPPKKTNETGRPSVKIE
                     PKRVDSVNAPNAKTYTVKAGDSLWSICQKQLGNGSLYKKVYELNKSMMDKANKGKKVP
                     KYTIYKGQVLRLV"
     gene            36534..38507
                     /locus_tag="PC414_001541"
     CDS             36534..38507
                     /locus_tag="PC414_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDF2463939.1"
                     /translation="MSDELVLANDRDVRLVIAHWEDFYEPVVLDGITWEIERRGTPSK
                     LEFTIVMDDILEFCEGNSVRLYYKGVGIFYGYIFQKKRDKENHIKIVAYDQLRYFKNK
                     DTYVYSNKTASELVKMLAKDFNLKYNVIEDTKYKISRIEENKTLFDMILTALDDTLRE
                     KKEMYILYDDFGRITLKNVASMKLDTVMNNDVIEDFDYNSSIDSDTYTKIKLVRDNEE
                     TGKRDVYIAQDSTHMRSWGILQLFDTVDKNMSEAEIKQKCDILLKLYNKKTKSLSLKN
                     VLGDIRVRAGCLVPVFLDLGDIDLQNYMLVEKVKHTFENNSHFMDLTLVDGDEFASYS
                     SSSYSSGNTNNKDEKKNGPAQSTTSKEDNDMINKLNKVFKNKLSNTGSIFVKYSNAYK
                     VNAALMAAISIHETGNGSSSLCKNKNNFFGMKGMSFGSVDEGIKRGISNLSRNYIHMG
                     RKTLESIRDKYAPLYDSPLNKHWVPGVNKFYKQITGNAYSSNSAGTGVRSNEEAEKNL
                     KDITYQVQNSNNANTSTNNNSKVNKVIQEAKNQLGKPYKWGGNGPKSFDCSGLMVWAF
                     KRGAGINLKRVSADQSNDSRGKLLCNINDVKAGDLVFFAYNQGKGNVHHVGLCIGNDK
                     FIHSPNTGDVVKISSLSGRQKQKHDFARARRFF"
     gene            38521..38781
                     /locus_tag="PC414_001542"
     CDS             38521..38781
                     /locus_tag="PC414_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 domain-containing protein"
                     /protein_id="HDF2463940.1"
                     /translation="MSQDLLQIIKKAAMDAVETSNPMQIAFGTIESVNPLVVKIEQKA
                     SFEESFLIQTDTFKRYTDKKIGDKLVLIRMQGGQQYLILDRM"
     gene            38786..39205
                     /locus_tag="PC414_001543"
     CDS             38786..39205
                     /locus_tag="PC414_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HDF2463941.1"
                     /translation="MLPTDNIDYDIEDVSIINFDVRQEPSKTFKLNIEKSKIDGICDD
                     VEALKQTIFLILNTERYQHLIYSWNYGVELNDLIGEPISFVIPELERRIKEALIQDDR
                     VENVDNFEFQNVKGKVHCKFTVYSKYGNIKAEKVVSV"
     gene            39206..40255
                     /locus_tag="PC414_001544"
     CDS             39206..40255
                     /locus_tag="PC414_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HDF2463942.1"
                     /translation="MFELMTFENIIKRMLDSVPDTFDKREGSIIYNALAPVAIELTET
                     YIAMDELLDQTFVDTASYYYLEKRCKERGITPLPATNTIAKGVFNIDIPIDSRFNLGE
                     YNYVAIERISEKTYKMKCETTGPVFELGQLIPIEYVDKLETAELTEILINGEDEESED
                     SLRQRYYDSLNSQSFGGNMQNYKDEVNKIQDVGGVKVYPVWDGGGTVKLVIINSNFKV
                     PSSDLVNLVQEEIDPLQNQGEGLGLAPIGHRVTVEGVTSTTINISAEITYKNGYTWEN
                     IKSIAEEAIDDYLNELNMSWEDEENLIVRISQIETRLLSIDGVLDIANTMINSIASNL
                     NVDKDSIVLRGEVVG"
     gene            40248..40865
                     /locus_tag="PC414_001545"
     CDS             40248..40865
                     /locus_tag="PC414_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HDF2463943.1"
                     /translation="MDKEINLINYLPQILRDKEEYIKVFNVENKEIKTLHEKLNDLSN
                     DQFLEDLTPSGIKRWEKIMSITPKSNESLEDRRFRIFSKYISKLPYSERFLRNWLDNV
                     VGEGNYELTINNATYNIHLESDARNQDWFEEVHSFVSNIKPCNMTLDYTRVLISKDNY
                     MNFGITTLMGQEITIYPWSPPDIETYGEIDVLTGNGVGYQEITIF"
     gene            40877..41869
                     /locus_tag="PC414_001546"
     CDS             40877..41869
                     /locus_tag="PC414_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDF2463944.1"
                     /translation="MAIDKSYYTIITDVGKAKIANASVTGNKVGFVKIQLGDGGGSEY
                     TPTESQTALKNVVWEGNIGNTTTDETAPNCIILESLIPSSVGGFMIREIGYLDDENNL
                     IAISKYKECYKPSIEQGAVVDMKVKTVLIVSNVNNIELKIDPTIIFATLKDIQDLETK
                     IGTVNTKIDTTKTELNTRIDTENEKQNIKIDQLIAGGSNVSHTHIIEIDDWILNNETN
                     MYEVTINHPLLTKRILIALYDEIGEALTPNARAIDDNSILVRNEENIKMYVYLINGNA
                     ETHFINATVDDNRVSEMTTYSSKKIEDRFLNLEEKVNGGLSSIATSVNELITYC"
     gene            41886..>42719
                     /locus_tag="PC414_001547"
     CDS             41886..>42719
                     /locus_tag="PC414_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463945.1"
                     /translation="MQTEWNFGYNGSPQSVILKPGKYKFECWGSSGGINNSSWHTDAK
                     GGYSKGEITLKKQTTLYVYVGESGFASSSTSNNTKSGFNGGGKGYLNQQVMGTYYSMY
                     GGGATDIRLVGGAWDDEQGLLSRIIVAGGGGGSYSPYTGGAGGGLAGGTGYSANDRHR
                     PGGTQYQGGIGRVSTENGSFGKGCSAKDSTGEGGGGGWFGGAGMNGAGAGGGGSGYVL
                     TKDSYKPTGYTPTSEYYFDNVVMESGGNTAGAYGYAKITLLQALPFLTVSSYNSITAT
                     FK"
BASE COUNT        17433 a         4464 c         8071 g        12751 t
ORIGIN      
        1 aaataaatac atcaagtcta taagttaagg aatttttata ccatttttta cgaacataag
       61 ttctgaaaag ggatataaaa tataatatta taaaaagaag gtgattggct aaatatttta
      121 catatttaag gggcaaggaa taaatattat aaattataag agggtgacgc aagtgggaaa
      181 ttcagattgt tctaaaatgt tggcatttta tgaaattatg aaaatgttag agactattga
      241 tgaagctaaa ttttataatt gtaaagaaga aataaagtca gagtttaagc atataaaaaa
      301 agaggattga atcctctttt tttatatatt tttatgttta ttctttattt ttagtatttt
      361 gctcctccaa aaaaagtttt gctatcttta aagctttatt tttagaatca tcatcaagtt
      421 cactaaaaat atcaagtatt tctttcatgt catcagatgg atttagcttt ttaataagtt
      481 ctttttctgt gatattagaa atattaattt tgatattagt tctacctaat aaatagtcag
      541 ttgatatatc taaagtatca gctatatgtc tcacaacatc aagcgctggg cttcttttac
      601 cagtttcata tccgttataa gtgtttgctg gtaaacttaa tttattagcc atctcttttt
      661 gcattaatcc gagttcttct ctctcttctc ttaatctttt agaaaatata ttgctttcat
      721 ctttatacat tgtaattgtc cccctcttaa aaactataac gaatgtaatt actcttattc
      781 tactacaaaa tacgcatttt gtgattttaa ttcgcaaaaa ggaagaaaaa aacaaaaaac
      841 gtattgacaa ttcacaaaac ggagtatatt atataaatat agaaacttca aattgagaat
      901 aaaaggagtg atatatattg aaaaattata tattgaaatc tcttagagta agacaaggat
      961 tgagacaaag agatttagca aaaatattag gaatgaatcc cagtacttat tcaagtaaag
     1021 aaaatggcga taggcgattc acagtaagtg aagcaataaa aatatcagat ttttttaata
     1081 cagatttgag agatattttt ttaaataaat aattcgcgaa atgtgaataa aaaggaggta
     1141 gataaacatg aataacttac aagtaataga aagaaacaac aaaagagttt taactacaca
     1201 acaactagca gatgtatatg aaacagatgc aagaaatata agcaacaatt ttaacaataa
     1261 caaagataga tttattgaag gtaaacatta tttttgtctg caaggtgatg atttaaggat
     1321 atttaaacgc ggttcatatg atataggaat tgcacctaat gtaaacaaac tttacctttg
     1381 gactgaaaga ggagcaaaca gacactgtaa aatattagat actgataaag cttgggaaca
     1441 gtttgataac ttagaagaaa catatttcaa agttaagcaa cagaagctaa cttgtataga
     1501 agatgtatta atagaaagtt taaaagaaat gaaagattta agacttcaag ttaaccaagc
     1561 taacaatata gcattagaag caaaaacaga ggttaaaaca ataaaagaag ttgtttcatt
     1621 aaatgctact gactggagaa aagatacgca acaattaatt gcaaggatag caaagaaaca
     1681 aggtggattt gaacatataa acatgcttag gagagaaagc tacgaattac taaataagcg
     1741 atttggagtt gacctacata gaagattaat caataaaaga agaaagatgg cagaagaagg
     1801 tgtatctgaa tctaaaagag agaaagttaa caatttagat gtaatacaag atgataagaa
     1861 actcatagaa gggtatgtgg ctattgtaaa agatatggct ttaaaatatg gaatatcaag
     1921 tgatttaagc aaaaattaag ttaaatcaca acagcacttt gaaaactaaa tacagaatat
     1981 tttgaaaagg agtggttaaa ttggaaaata aaaaaataga agaaataaaa aatgcattaa
     2041 taggggtgaa tcagatagag tggtctttag taaaatcttt tattgatatg tattttagta
     2101 aaaaagccgc caaacttgaa attgacgacc tagataattt tgatttgtat ataaaacgaa
     2161 aattttaatt actagcaatt tgtataaaga ttggatggat tctatagtca acacccttat
     2221 agtaaatatg aacataatct tgctgataca tagtatgttc ttcttcttta ttagaaggac
     2281 tccatacatc agcaccttct tcccaccata tgtaaggcga agaatggtta ctacccattt
     2341 tacattctgg gtcatcacat agattaaccc aattaccagc taaacaagca tatattttag
     2401 tcataatatc accaactttc atataaattt ggaatatatt ccatatttat attataccat
     2461 gtagaacgaa ggtgattaag tgttaatagg cgacaatata agtcaaatac taagaaaaag
     2521 aaatataaaa ccttataaat tagcaaaaga attaggaata gatgaaagtg gattatacaa
     2581 aattataaga aatgaaaaga aaaatccaac cataaacact ctaataaaaa tagctgatta
     2641 tttagatgtt acattagacg aattagttgg aagatagaaa ttaaatacag aatattttga
     2701 aagtaggtgt attgtatggc aaaagcagta gctaaagaac cattatttta tagagcaaaa
     2761 gatgtagcta aatttttaga tatatgcgaa gcaactgcat acaaaataat tgcagaatta
     2821 aatgaagaat tagaaaaaga aggttttaag actttttctg gcagggtatc agttgcttat
     2881 tttaaagaaa gatattgtta taagccaaga aagggggtga tttagttgaa tgtaagagta
     2941 ttaatagctt atatacagtt ttgtaatgat aagcaaataa aagcaagttt tgaaggtctt
     3001 agaaaataca acagagatat agagttagtt aacttgtatt taccagcaca tttaaaaata
     3061 aagtaagcta taaataatta aagaaaaagg tgattagatg caaagagagc agacaacaat
     3121 acgcctgcct aaagaactta aagaaaaatt agaaaagcag gcgagtaaaa aaggaagaag
     3181 ttttaatagt attttattaa gcatattaca agaatttatt cagaatccaa atgtatagga
     3241 ccatgttctt tttcaaacat ttctatatgt ttagtgacaa gaaattctat ttctttgtta
     3301 gctgaacgag cattatactc agctatatat ttaagtttat caagtttttc ttttggtatt
     3361 ctaattgcaa aatgaggatt tttatatgta cccattttag ccataatatc aactcctttg
     3421 tgtagtctat ttggcaacaa ttatatcaca aaaataaata caaaaaaata tataggcaac
     3481 atatagacaa cataataata aagtgttata ctttgtatta aagataggca acacatagat
     3541 aacaatatag gaggaaatta tgttggcaaa tagagtaaga actggattaa gaatcccata
     3601 tgatttaaat acaaaattaa tattaatagc agaagaaaga ggaatgtcaa aaaattcttt
     3661 aatattacaa atactttggg attacataaa agaaatagaa aataaggagg tcaaataatg
     3721 gttgaattgg taaaagaatt tgatttacaa acaattaaag taggaaatgc agtaaaagta
     3781 aattgcaaaa gatttggttt tgaaattgat tgtatagtag tagtagcaac agaaaaagaa
     3841 ttaaatttag catactttga tgaaggtaga ggctgtatgg agtatcaagc cttaataaca
     3901 gaagatattc aagatggtga ttatgagatt aaaattttat cttaggagga aataaaatgg
     3961 taggtttatt tgcaatatgt ttagcaggat tatttcaata aaaaaagtgc tggtcaaagt
     4021 aaccaacaca tacaaaaaat tcaacttatt taggaggata ccataaaatg aataaaattt
     4081 caagtcgtag aaaatattta gatgctttta tagtaactga tactaagaac atagataaaa
     4141 ttgattggct taaaaataga caattaggaa tagggggtag tgatgcgtca gcagtagcag
     4201 gattaaatcc ctggaaaact tctgttcaag tatatataga aaagaaagaa gaaataccaa
     4261 tagaaactaa aagtttcaga atggaattag gcaatagatt agaaggatta gttgcagaac
     4321 tttttacaga agaaactggt cttaaggtcc gtaatgtaaa tggaatgttg aaaaatgaaa
     4381 agtatccttt tgcaatagct aatatagaca gagctatagt aggagaaaaa gcatttttag
     4441 aatgtaagac aacaaatagt ttttctataa aagaatggga aaatggagtt ccacttcatt
     4501 atgaaataca atgcttacac tatatggctg tcacaggagc tacacattgt tatatagcag
     4561 cacttcttgg aaatgaaaag tttgtatggc acaagataaa tagggataat gaagtaatta
     4621 aaaatctaat gaaaatagag agtgaatttt gggaagaaaa tgtattaaaa gacattttac
     4681 caattcctga tggttcagat gcttatagtg agtttctgaa aacaaggtat aaaaactcag
     4741 taaaagagaa aatagaacta aatctacttg aagatggtat atcaaagtta aaaagatatg
     4801 atgatatagt tttacaaatg aaagaactaa aaggagagaa acagctaata gaacaagaaa
     4861 tacaaagtga aatgagagag tttgagttag ctacattagg cggaagaata ataacttgga
     4921 aaggagctac taaaaggtcc attgatacca agagattaag agaagaaatg cctgatatag
     4981 cagaaaaata tacaaatata agttcataca gaacattcaa aataaaatag ggggtaatat
     5041 atatggctag tgaaaaagca aaaggagcat tagaaaagaa agtttcagga gcaaatacag
     5101 tcaaggtaag tccaagtaaa ggtatggagc aacttatgaa taaaatggca agtcagataa
     5161 aaaaagcttt acctagtatg gtttcaagcg agagatttca aagagttgcc ctaacagctt
     5221 ttagtaataa tccaaggtta caatcatgtg aacctatgag ttttatagca gcaatgatgg
     5281 aatcagctca attaggtctt gagcctaata cgcctttagg tcaagcatat ttgataccat
     5341 atggaaataa agtgcaattc caaattgggt ataaaggtct tttagaatta gcacaaagaa
     5401 gtggaaagat aaaaactata tatgctcata aaataagaga aaacgataaa tttgagatta
     5461 aatatgggct tcatcaagac ttagttcatg aacctaaatt aaatggtgat agaggggaaa
     5521 taattggata ttatgcagta tatcatttgg atacaggagg acatagtttt tcttttatga
     5581 ctaaagagga aattatagaa tttgcaaaga gtaaaagtaa aagttatagt agtggaccat
     5641 ggcaaacaga ttttgattca atggctaaaa agacagttat aaaacagtta ttaaaatatg
     5701 caccacttag tatagaatta caaaaagcta tggtaggtga tgaaacaata aaatctgaaa
     5761 tagatgaaga tatgagcatg gtcgtagatg aaagcgaaag tttagaagtt gatttcgaag
     5821 taaaagaaaa tatggatggt aaagttagtg tggaagaagc tataaatgtt gattaagtag
     5881 gtgaggcacc ttgaatgaag ataagtcagt tatagagaaa ttaaatatat taagtggtgg
     5941 atacggtctt atgccaagaa taatagcaag agataggtgg ttgacagttg gcgctagaat
     6001 gctgtattca tatctaacta gttttgcagg gaatgatgga acatgttttc catctaggga
     6061 tttaatttgt tatgaactag atatatcaaa agacacattt acaaagtaca aaaaagagct
     6121 agagatgagt ggctatataa gggttcataa gaataaatcc aaacaaggca agatgcaaaa
     6181 caatatatat gaaatagtat ttgatagaac ttatatagat gaatgtattt ctaagagagg
     6241 tttaaaagag gagaaaaaga agaaaaagcc atgtactaaa aagcaagaca cggaaccgta
     6301 tcctaaaaat gtagacatgg aaccatgtcc tacttttccg gacacgactc agccggacac
     6361 ggaaaatatg gacactaata gtaacagtat taatagtaac agtattaata atatgtatat
     6421 agtaaagcaa cctgtggata actttttaaa agaatttaag aagctgtatg aagaaaatat
     6481 aggagtaata tatccagtta cagctgaatg gttattagaa gtatctaatg aagtagatat
     6541 aagagtattt aaaagagcta tagagatatg tgctgaaaag atgaatatga atctagcata
     6601 cttaaaaggt atccttaaaa aatggaagga tgcaaatatt actacatatg agcaactgga
     6661 atcatataga ttacagcaag aaaataagaa aacaaaaaaa gtagttaata accatgtgag
     6721 taaaaataag tttgctaact ttgaacaaac atttactaag tattcagaga atgagcttga
     6781 tgacattatt aagaaaagcc aaaaagagaa gtttggagta ggaagttaaa atattggagg
     6841 gatgaaagat ggagttaaaa tttagagaat ggaataaaaa tggtaaagaa atgtatagtt
     6901 atgatgaaat ggtgtgctat tctaaaaatt tgcttagaga gtgggtttat agtggtgttt
     6961 atttaccaac aagcaatgaa aattttgagg ttatgatata cacaggtttg aaggattgta
     7021 ttagaaaaga aatctatgag ggtgatattg tttcatacat cttatcattt gaagaattta
     7081 taggagaggt aaaatttgaa gaaggtttct ttgtaataga taatgaagtg ctaggagaat
     7141 gcgttgggtt atttcatgaa attgcagtgg ttaaagttat tggcaatata tatgaaaatc
     7201 ctgagatgtt agaaaagata aggaaaccta aagtgttgga ggtctaaaag tgaaacatga
     7261 gtgtaagaaa cttttcttgg aatgtgataa gggaagtttt gagataaatg atacaagaat
     7321 tggagaagta atgttcgaga gtacagaaat agacaatccg tttgagagag taaaatatga
     7381 aggtaaagct acttttgaaa tagtatctgg atgggagtat ctacaaagag aaatgttgtg
     7441 gcttaagata ttgcatttat cagcagttgt agcaaaaata atgcaatata aaatgttagg
     7501 tattttaata agggagatgt aataaaatgg ctaaaatttg gatggatgca ggaacgtttt
     7561 tagaaaaaac tattgatata gaagatatgt ttgaacttaa tttaagagca ataagaaaga
     7621 gaaatgaaaa aataaaaaat gaaattgaga aacaaataaa atatgaagat tcagaaataa
     7681 aaactacaaa tcgtggtgga ccaggaaaaa ttataaaaat tttcaatata aatactggag
     7741 aggtaaagat tcttaagagt gctgaagaag caagtagata tataaaggtt agtcgtagct
     7801 atgcaagtta tttagctaga gaaaataaat caactgagga tggttggaaa gcagagtata
     7861 ttcaaggagc gacagatggt attagcaaat gtggaacaag taattaagtt agctgaaaag
     7921 atattaaata agaaaaagtg ttctgttaat aaagctattg atatagctat aaaaatatta
     7981 agtaggtatg agtgtgaggg gatgttagaa aaatgaatat attagctatt acaatgctta
     8041 taataggaag ttttatagct ggtagagttt atgagtatag attgaatcta aaagagtgtg
     8101 aaaattgcga caataaaaga ggtgtataag aatggatgat agattagaaa ttataaatgc
     8161 ttctgtaaat tatatacaga tgatatgtga aagttcaaat atagctatta tagcagagcg
     8221 aggaagagtt agaatattag atttagaaac taaagaaaaa tatgatttat taaaaaataa
     8281 actcgaggaa atgttagaag aaatataagt gaaaatatct aattaagaca gtttagagag
     8341 ttgcaaaatg tcttttggta taaattattg ttgaagtgtt ttgtgactct caaaaatgaa
     8401 aatagagagg ggaataatat aatggattta aataaaatta tgaatgatgc actagtagag
     8461 ttagaggaaa atggctttgt agaggaagta gttaaaaaac aattggcaga aacaataaaa
     8521 agagttgtga atgaggtttt tggaaattgg ggtgatttta gtaaaaaaat tgaaaaacat
     8581 ctaagtgaaa atataaatat aaatttagat aaattagata tacaaaaata taatgtgctc
     8641 gtagcaacta cagtaaaaga aaagattgat atgacaatga aggtagaagc tattgaacac
     8701 ttaaaaagaa atttagataa catgcttgta ggtgctgaaa aagaatataa aatgagcgaa
     8761 ttgttggaaa tattaaaaat ggataaatat gatattgatg aatatgaagg ctatgaagat
     8821 tgcataacat ttattcttga tgaaaataaa tatggttcgg gatggattga aattgataaa
     8881 gaaccaaata aaagtaaata cagatgcgaa cactccatat tgttgagaaa agatggaact
     8941 attgcatcat atagatacga gaatagagaa atatcatcta aagatataat gagtggattt
     9001 gataattttg gagatttgct atttaaaata tttgctcatg ggtcaaaaat aattttggat
     9061 ttaggaaatg atatagaaga ttatgattta acaaatggag aagattatta aaatagggga
     9121 tggaacaaat gtcaaagtat gtattgagat ggcaaaaagg attattacta gatgaacgca
     9181 agataaatta ttcatgtgga agtatagaaa atttaaaaaa gaaagctgaa ttattagcta
     9241 aagatgacaa gatactgctt gtaacaatag ataaagttga agaagttata aaagatacta
     9301 gaagccaaaa aatggctgaa tattttgagg atgaaggggt ggaagtatga taatacacaa
     9361 atttataata catgttttag ataagaatag cgacacacca atactaaatg attttgaggg
     9421 tagagtcagc caagatattg aagctttctt tcaaaagaaa ataagcaaag tatcaagaga
     9481 taatgacatc agaacagcag tatttaatga ctatagtaac aatctaatta agaagtgttg
     9541 tgaacaaatt atttatgatg aaagttcatt tttaaataac tctaaagaga ttgcagctta
     9601 tttatttgat gttatgaaat tgaatgctac attagaatct tgcgacttag caatttgttt
     9661 atactctcaa aaagatgaaa agaaagttgc tatattaaag cttgattaca ataattcgta
     9721 tactcattca attgagttta aagatgataa atttaatata cagatgtcta aaaatgaaat
     9781 taatatacaa gagactaaga cggttaaaat tgctgctttg gttggattga gtggaatgaa
     9841 tgacaaatat catcttaggg ttttagacaa ggatgcagag aaggaagaag ctaattctaa
     9901 gtttgttaca gagttcttaa atgccactaa gataaaagat gataagtata agactaagaa
     9961 gttcaaaaat acagctgaga attggataac taatgctctt agtaatgata taaaacaagc
    10021 agaggatgta agaagtatat taaattatac tttgagagaa aagcatgaaa ttgatataaa
    10081 tgattttgtt gataaaacaa ttaaagatga taagttaaaa gatagtttta aagaacatat
    10141 ggaagaaaaa ggtcttgttg aaggatttag tatagataaa aaatgggttg ataaaaagct
    10201 taaaaagaga aatataaaaa ctgacaatgg ctttgaaata aaaggtaact taactgattt
    10261 tgaggaccca atgaaatata cagtaagaca aaatcaaaat gggtctatag atatagttat
    10321 taagaatgta acattttatg aggaaaagta ggtactcatg tgactattgg ctagagaagg
    10381 agaagtaaat aataagagga tgtaaattta aactagttag gaggaataac ttatgaagat
    10441 ttttttattg actatactgc taataattat ttgtatatta gcaaattatg tgaaaaatcg
    10501 catatataaa aaatctataa ataatctaaa atataaatat tctgtagggg aaaagattat
    10561 atatcatcaa ataaactgtt actataacag aatggttggt tgtgaaattt tagaaaaatg
    10621 ttatagtacg aaatttagaa aaagaaatac cccgctttat aaagtaaaag catatgtagg
    10681 tgataacgat acaacatggg ttataccaga gtggagaatt gaatgtcttg ctacgactta
    10741 tggagaattt cctaaatatt aaataataaa aattggctgg agaaggagat tgtaaattat
    10801 gtttaacatc tataaagtga aaataaagac taaaagaaca ttggagcagg taagaaatca
    10861 aagcgtagac tttgagtatt cagaaaaagg attaaaaaat actctgaaat actataactt
    10921 gattgatgat ttaaaagtaa tagtagttaa atttggagat gaatattgtc tagctaatta
    10981 caatgaagaa gatagaaaaa taataatgga agcacattat cttttagagc aggatgaata
    11041 tactggatgt tatataaatg aatatgaacg atttaaaaaa gattgggaaa atggtaattg
    11101 tgatggggaa gcctgtatgg tattttcaga tgatgaaatt gagataattg agaagctaag
    11161 ggagggttaa atatgaataa aagaattaaa atgaaaaaaa gattaattca taaaaagtgt
    11221 gatgaaagat gtgtcaacta tgactttgta attagcaata accttataac ttgtaatgtg
    11281 tgtataggat gcaaatacaa agaaaatatg gataaagtat gtgaagagaa ctataagaaa
    11341 ttaagaagta aatagaataa aatagtcaag gtaagtttgt gaatgaaact agaatgttat
    11401 aggcttgact tataaaagga gtgcgttaaa tggctaatat atattgtgaa aattataatt
    11461 gtaaaaacta ctttgaagat atgtgtatgc ttgaaagaat tgaaattaat aacttcaaag
    11521 aatgcgaaag ctatcttgaa ggtaaaaatg agctatatga attagaaaac ggatatacta
    11581 tacatcctaa agatttgaaa atggtgaaaa gtaaagatta ttctgttgaa gttactcata
    11641 ttccaactgg tattacagta aaatgccgtt ctacaaatag tattttaaaa aataaaaata
    11701 agtgtttgga agttctagaa gaagaactaa caaaaataaa ctctcactta gagctagaag
    11761 atttacgcta aataggaagt gagcttatga aacgaagaag atgcagttgg tgtggtaagt
    11821 tattttatct tgaagaaaaa tctaaggatg tttattgttg taaggaatgt aggaagaagg
    11881 ctaataagaa aaataaatag tggaggtatt aatatgcaaa aagatgtttg gttatatagc
    11941 tgggatgatg agtgctttgg aagtgatgaa tacgaaagta aagaggaagc tattcaagga
    12001 gctaaggaag aacttagaag gtttggggag gtcagtcgat tggtttacgt tgggcaaaaa
    12061 gaagaagtta atatacctaa tctaaatgta gaggatgctt tagaacatgt tcaagataga
    12121 attgacaatg aatttatggg gtatggagaa gattggtttg aaaaaatacg tgttgaagat
    12181 atattaatac tagagaatag aataagcaaa gtatttaaaa aatggataga tgaatttgga
    12241 tataaaccat attggtttgt tgttagagat acggaagaaa tagaactaaa tgaggtagca
    12301 aatgaaagtt aattttgtaa tagatggaga gccagttggt tttaaggagg actataaata
    12361 tgaatgagat tatgacaaat gaaaatttaa gagttgtagc agatgattta gttacagttt
    12421 atgaaactga tacaggagag aaaatagttt ttgcaagaga actacataat aacttagaag
    12481 ttaagagaca gtttatagac tggattgaag atagaattaa acaatatgga tttaaggaaa
    12541 atgaggatta ttcagttttt cacaaaaata tgaaaaactc aactggtggg agaccttcaa
    12601 aagaatatgt attgaagctt gatgtggcaa aagaacttgc tatggtacaa aacaataata
    12661 aagggagaga aataagaaga tattttataa gattagaaaa gttgctcaat agaaccttat
    12721 caaattcaca gcttagtcaa attaatacta ttgtgaatga atcattactt aagatggaag
    12781 ttaaacataa tatccaaata gagcaactta aaaaagagtg ttcagaatat tatagaccaa
    12841 cctctaaaac taaatatgat atatcttctt atataaaaga aagactaggt atatcaaaag
    12901 ttaatgaaga atttgaatta gttaagaaaa gaactctatt ggtattaggt gctgacaaat
    12961 gggaggatat cccgaaagat gtattactta attcattaaa cttaatagat gaatcaatta
    13021 gaattataaa atctgaaaga aagacaaatc agattagttt ctttgaaaaa gataatttct
    13081 gttaataaaa agaaaaaagg agtgctttca cactccagtc gtcaaaaata taaaactttt
    13141 atccaagatt attataacat aaacaggagt gtggaagtat ggataataat atcaataaaa
    13201 aagaactatt taaaaaagta gaaggtagac tacatcatta taaattttta agtgcagaaa
    13261 ttaaaaatct tgaattagat atagaaagta gagaaaatga gatatttggg tgtaaggctg
    13321 ttggatatga tgaaaaagta agtccaacat atgcttttaa ttcaagtgtg gagaatgaga
    13381 ttataaaaaa agaaagagat attactagat tgaaaaaact gaaaaaagat aaggaaattg
    13441 aaaagaagaa aatagaaaat gcacttacat gcttagatat aagagaagaa cattttttta
    13501 aactgtttta taatagcaga atgaaaaata gtatggttta tatatcctta gagatgaact
    13561 cagatagaaa aatatgtaga tgtgtgaggg aaagattagt gtataaaatt atggatatgc
    13621 tttatccaag aattaaggaa aatgaactac cattatttaa aaattagaaa attccccagt
    13681 ttttccccag aaattcccac tttattccct actttctccc ctttttgatt aaaaaagcat
    13741 gagataatag tattgtggaa ataaagattt ccctctcaaa actaaataat tgctaggtta
    13801 gtttaaaggg ctaatctagc aatatgaaca gactaggcag ggcatgagga tgctgttagt
    13861 tcaattctaa ctatgttcaa aacctattaa tacactatat gtagcagttg aattaagact
    13921 aaaatctcat acaattttat cttaattcag agtctaaaaa ccgagtgggg cttggtaacc
    13981 tcactcacca tgcaggtact ggtgtttaat ctaggttcga ttcctagaac ttgcgacata
    14041 atatatgtat ctccctattt aaaaaggcta agtgagggta agcttagtct tttaatttaa
    14101 aaaaaaaaga aatttttatt gtcataatac tatttgctta ggtatattat aatgtgcata
    14161 cttaaaatta aatacttagc aagcatttga attaatatac ataaaatata tgacataatt
    14221 ttttatagtg taagttattt aaattaatat aaataacagt aattttatta tagaaaatgt
    14281 atatattgtg aataataata ataataaaat cttgtacaaa atgccaactg ataattcctc
    14341 gaaatgtatt gtgtatttaa cgttacgatt gtataattaa cttataatag tgatatggaa
    14401 ggtggtactt atggctacaa aaagtatttt aaaaaatgta gatgtaagaa aaaaggcatt
    14461 tggaagaaat ctagtatcag ctctagaaaa tgctaaaaat aaacaagaaa aagaagttgt
    14521 attaagtaaa aaatgttcag aagtaccaaa agataaaata aaggatatat tcgggagatt
    14581 ttaatgagtg gctatttaat tgtaaactta agtaatatgc tgggagaact ggaggaagaa
    14641 gaagttaaaa aaattctctc cagtttttct tgtcctctta ataaagatgt agaagacttt
    14701 ttaaaaaaca aagctattga attttctaaa cagggtttag ctagtacaca tttagtatta
    14761 acttcttata aaggcaagcc tgttatagtt ggatatttca ctctagctaa taagtatttt
    14821 acaataaaaa gaaaaacatt aacaaactct ttagctagaa aagtagtaaa gtttggacaa
    14881 tataatgaag aactaagaag atatattatt ggagcacctt tgataggaca aataggaaaa
    14941 aattattcaa ataattataa taaattaatt aaaggtgatg aacttctaaa aattgcatgt
    15001 gacaagataa aagcagtgca gttagatatg ggtgggaaaa tagtatatct tgaatgtgaa
    15061 gacaaaccta agttaattga attttataag gataatggat ttgtagattt tggtaaaaga
    15121 agtcttgata aagatgaaac agattcattg gatggggact atctagtcca aatgttaaaa
    15181 tatctaaaaa aataaaagta cataaaatct aaaatgacta tcttgatgga tggtcttttt
    15241 ttatacaata aattaaaagg agaattatag aatgaaaaag ataactaaac ttatattatg
    15301 ttctttgcta gtaatcagta tagcgagtcc attacaagtt gaagctaaaa ctactcatca
    15361 ttccagttcg catgtttcta ttcataagag tagtacaaaa tctaaaagta gtagacctaa
    15421 aattactaaa actaaaaaag ctactaaaac atatagaact aacaaatcta tcaaaaagaa
    15481 aataaaactt aaaaaacaag taaacaaaaa aactcaagtt ccaaataaac aagctgctag
    15541 aaaagacaaa aaaacaacta catataatta ttacaatagc tataactcaa atagatacta
    15601 tactacttca agtagcgggt taagtttctg ggattgctat atgttaggtc atttattcaa
    15661 taacaacaat aaagtttcag agcaagatat agctagagag ttgcaaaaac aaggttatag
    15721 taaacaggaa actgataata tattaagaaa agcaaatcaa aatcaagcta aaaaagaaga
    15781 aaagaaaaaa agagtgatga tgttaatatt ctctgtatta ggagcattgt tcattatagg
    15841 tttagggcta ataatattta tcaaaaggga atcaaaaggt tgcaataaat caatgtaatt
    15901 taattagata catagactct aacaagagtt cttttttatt cccaaaacga caaacaaacg
    15961 aggtggtggt atgaatgaaa aggcagattt agcccatgaa gattacttaa aagggcttaa
    16021 gtacaaggaa atagctgaaa agcataatgt aagtttatcg actgtaaaat catgggcaac
    16081 tagatactgg aaacaaaaag gttgcaacca atcaaaaaaa gttgcaacca aaaagagagg
    16141 tgcacccata ggtaataaaa atgctactgg tccaccaggt aataaaaatg ctgaaaagtt
    16201 tggtttcttc tcaaagtatc tacctgaaga aactagggaa ttgatgcaag aaatatctat
    16261 aaaagataaa ttcgatattc tttgggaaca gataacaatt caatatgcag caataataag
    16321 agcacaaaag ataatgtatg ttaaagacaa ggaagaaatg attaaagaat taaagaaaca
    16381 tgaaagtaca gaaaatggag agaagataga gtatgaattt caatttgcat gggataggca
    16441 agcatctttt cttaatgcac agagtagagc tatgagtgaa ttaagaagtt taattaaaca
    16501 gtatgatgaa atgattcata aggattggaa tttggctaca gaggagcaga aaacaagagt
    16561 tgagaagttg aaatgtgaag ttgataacct aagtaaagat gacattggag atgatgagtt
    16621 gaaaataagt gtagattatg gtgatagaaa tgatagttag agtaaatttt aatccagatt
    16681 tcaaggaagc caattttact aaaaaaagat acagagcaat gaaaggttca gcagggagtg
    16741 gaaaatctgt taatgtagca caagactata tactaaagtt aggagataag aagtatcaag
    16801 gagctaatct attagtagtt agaaagtcag aagctacaca taagtattca acgtatgcag
    16861 agcttacagg agctataaat cgtatttatg gtaaacaagc tgataagtat tggaaaacta
    16921 ctttaaatcc tttagaaatt aagagtaaag ttactggtaa ctctataatt ttcagaggag
    16981 ttaatgatgc aaaacaaaga gaaaaattaa aatcaattaa cttctcgaaa ggaaaattaa
    17041 catgggtttg gtgtgaagaa gctacagaac ttatggaaag tgacatagat atactagacg
    17101 accgtttaag aggtatttta actaatccta acctatacta tcaaatgaca tttacattta
    17161 atccagtctc atctactcat tggataaaaa gaaagtattt tgactataaa aatgatgata
    17221 tatttactca tcatagtact tatctacaaa atagattcat agatgaggct tactacagaa
    17281 gaatgcaaat gagaaaagag caagaccccg aagggtacaa agtctatggt cttggagaat
    17341 ggggagaaac tggtggagca atacttaaaa actatgttat acatgaattt cctacggaat
    17401 ttgaatactt tgataatatg agactgtcac aagactttgg ctttaatcat gcaaatgtag
    17461 tactcagaat tggttttaag gatggagagt tatatatatg taatgaaata tatgtacatg
    17521 aaatggatac atctgaaata ataaagattg caaacagtaa aggtttagaa aagaatatat
    17581 ttatgtactg tgatagtgct gaaccagata gaattaagat gtggaagagt gcaggatata
    17641 aagctaaagg agttaaaaaa ggaccaggaa gtgttaaagc tcaaatagat tatttgaaac
    17701 aattaagaat acatgtacat cctagttgca ctaataccat aaaagaaata caacaatgga
    17761 aatggaaaca agatgaaaga actggattat atcttgatga accagttgag tttatggatg
    17821 atgcaatggc tgctcttaga tattctatag ataataagct taaaaataat ggaataagct
    17881 tcttaaagta aaggaggcgt taaatattta tataagtgaa acagatttaa taaaagttca
    17941 gttaaaaaaa gagagcacat ttaacctagt aaaagttata gagcattata ttttaaaaca
    18001 tagaccagag aaatataaac aaggtgaaga atactattat ggcaatgcag atataaacaa
    18061 taagagaaga tattatctct tagatggagc taaggttgat gattttacta aagttaataa
    18121 taaagcaatt aacaactacc ataagctttt agttgaccaa aaggtaggct atagtgtcgg
    18181 aaatcccata gtatttaatg cagatgatga taatctcact aagcttttaa atgacttact
    18241 aggagaagag tttgacgata caataacaga actatatctc aatgctagta ataaaggggt
    18301 tgaatggtta catccatata ttaatagaaa aggtgagttt aaatatgtaa taattccagc
    18361 tgaagaagca attcctattt gggatagtaa aagacagagg gaattagttg catttattag
    18421 gttttattat attgaagata tagatggaaa taaaataaaa agagttgagt actacacaga
    18481 aaatgacgta acttacttta ttgaaagagg taatagtttt attcaagaat ttttatatga
    18541 tgaatatgga aaaatgactg atatacaaga aggtcatttt agaataaata acaaagaaca
    18601 gggatggggt aaagttccat ttataccttt taaaaataat gaaaagtgtg tctcagattt
    18661 aactttctat aaatcattaa tagatatata tgacaataat atttctacac tagcagataa
    18721 cttagatgaa atacaagagg ttatttatgt attaaaagaa tatccaggaa caagtctaca
    18781 agagtttata gataatataa gatactataa atcaattaaa gtagatggtg gaggtggagt
    18841 tgataaacta gagataaata taccagttga agctaaaaag gagcttcttg atagattgga
    18901 aaagaatata attatctttg gtcaaggagt taatccagaa tctcaaaaca caggtgacaa
    18961 atcgggtgta gcacttaaat ttttatattc actacttgac ttaaaatgtt ctaagactga
    19021 aaagaagttt aaaaaagcaa ttagagagct tttatggttt gtgtgtgagt atttaaagat
    19081 aagtggtaat aagagctatg attataaaac agttcaaatt acttttaatc actctatgat
    19141 aataaatgaa gctgaaaaga tagatatggc agctaaatca actggaattg tatcagatga
    19201 aactattgtt tctaaccatc cttgggtcga ggatgttaat gacgaacttg agagacttaa
    19261 aaaacaggaa gatactcaaa aagagtatga tgatttaatt cctaataatc aagatggtgt
    19321 tatagatgaa acataaagat tattggagga agagatttga acaattagaa gaagctcaga
    19381 ataataaaag tataaaatat tatcttgaat tagaaaaaca atataaacta gcaatgtcta
    19441 atatagaaaa agatatactt atatggtata acagattcac tgaaaatgag ggaatatctt
    19501 tattggaagc taagaaactg ctaaatacaa gagaactaga agagtttaaa tggagtgtag
    19561 aagaatatat taaatatggt aaagaaaatg ctataaatca aaagtggatg aaagagttag
    19621 aaaatgctag tgcaagagtt catataacaa ggcttgaagc tttaaaactg caaatacagc
    19681 aacaagtaga agttttatat ggaaatgaac ttgatggtat tgataaacta atgagagata
    19741 tttatacaag tggatactat catacagctt ttaatgttca acaaggagta aacgttggtt
    19801 ggagtttaat gagtcttgat actaacagaa taaataaagt tatttctaag ccttggacta
    19861 gtgatggatt aaactttagt gaaagaattt ggggtaagca tagacctgct ttaatcaatg
    19921 agttacatac taagctaact caatcaatta ttagaggtga aaatccaaaa gttttggtaa
    19981 atgaattttc taagaagttt aatgtatcta aatcacaagc taagaattta ataatgactg
    20041 aatcagcttt ctttgcatca gcttcaagaa aagattgttt taatgattta gatgtagaaa
    20101 agtatgagat tattgccaca ctagatttaa aaacatcaaa tatatgcaga gagttagatg
    20161 gaaaagtatt tgatatgaaa gattatcaag ttggaataac agctccacca tttcattgtc
    20221 attgcaggac aacaacagct ccttggttcg aggatgaaga aggttataga gcagcaagag
    20281 atgaaaatgg gaaaacatat tatgtgccat ctggtatgaa atataaagaa tggtatgaaa
    20341 agacatttat aactgataaa aaagatgata tactaagtaa taagagttgg ttaaaagcag
    20401 atttcccaac tgaaaagaaa tttaaaaagc atgtagaaaa acacttaaat gaatatggaa
    20461 atataactgc tgaagaatat ttatctactg caagaaattt gttatcagag caattaagta
    20521 atgatgtaga aggattttta agtaaagata actttttatt taaatataga aagagtacta
    20581 atgattttgc tgttggaaga gctgatggta aaatatcaac cttatttaag ccaaaagaag
    20641 gttataatta ctggctagaa caaataaaag aatataagga ggtttagaca tgaattgtcc
    20701 agtatgtggt aaagatgtag atgtatttga tatttgtgat aattgtaatt ggcaaaacag
    20761 tggaccaaaa gaaactaatt acaaaggacc aaataaaatg tcattaaaag aagctaaaga
    20821 ggcttatgaa aaaggtaaaa aagtaatata aaagcacttg ctaagttata aattagtagg
    20881 tgcttttatt atgtaaaaat ttattgcgag ggtgatttga aatgcttaaa ttatatctat
    20941 acttatttta tcaataattg tgttttgtac agggctttat ttattcaaca tgaaggttga
    21001 tagtaatgag gaattaattg aattacttaa aagtaagaat atcagaagaa gaaaaaaata
    21061 taattttatt ttcccagcgt tatttccact gcttaatttt attttaggtg tgatactgat
    21121 actatttttt ttactagtta gcaatgaaga tatgattaaa aatttaaagg gggataaata
    21181 atatggctaa atttaaaaag aaatcagagg aagtagaagc ttttaaatgg atattaggaa
    21241 gtcctaatac tcctatatgg ttttatcaag cttttgagaa gggaactata tggcttgatg
    21301 aatctttaaa ctctgtgaat cgtaaaggtg aagtaaagaa aactatctgt ataaaatata
    21361 aaaatggagt tattagggca actaatggag attggattat aaaagatagt gaaggtaaaa
    21421 tttattcttg tacatgtgat gaatttgaaa agtactataa ggatttggat aatgaaacaa
    21481 agatttctaa tggaggattg gaatgtttaa gagagatagc attaaaagat gtgaatttta
    21541 aatttgaact cacaggaatt aaggaggaaa aataaatgtt ttatctgttg ttacaagtaa
    21601 taagtatatg tttaagtatt acagtattta tgtatgttac aggtaatata gtctttaaaa
    21661 tctatcttaa aagtttaaga aaagaatatg agtctaatat acacgaagtt tctgatgaaa
    21721 ttttaaagag aataagaaaa gaattaaata aatcattaga tgaaagtctt taagaagttt
    21781 tttattaggt agaaaacaaa aggaggtttc tgttattgaa agaatatatg atttggttta
    21841 aaagcggcaa tagcatatct ggaatagtag acgaagatgt tgctgataag ttaatgcaag
    21901 attttatgga agctgactca gattgtaagg acttgaaagt atatttagat gaagatggaa
    21961 caacagtaat agatttatca caaatagaag ctatatcaat aaataattgt aatgagaata
    22021 ataatattgg ttttagtaag tcctagatag ggctttttta ttttgtaaaa aatgaaagga
    22081 gatatttaaa atggattggt taaaagaatt gctagaagga ataaaagtag aggataacaa
    22141 aattgatgta gcttctcttc aaaaatctat agaaaagaaa ataaaagaga ctacaattac
    22201 taaagaagat tatacaaatc ttgaaacaca acttaataca gctaatgaaa ctattaaaaa
    22261 gtttgaagga ggtatgacaa aagaagatgt agagaatcta aaaacaactt atgaaactga
    22321 taagaaaact ttggaagaaa cctacaaaaa agaaattgaa gaaaaggact ttaattactg
    22381 gttaaatgat gcttttaagt ctattaaatg tagggatgaa atagcgttaa aagctcattt
    22441 agatatagaa gcactaagaa atagtaaaga tagacaaaaa gcttttgaag agcaaataaa
    22501 ccctttgaaa caggataaag attatttgtt taatgcaaca ctagaaggtg aagagcctaa
    22561 aatagatact ataacaccag ggcaagagcc taagataaat gattttggtt ttaattttac
    22621 tggggtaaga cctcatgaaa ataataataa ataggaggaa ataaaatggc agcactaaat
    22681 tatgcaaaag aatattcaaa tgttttagca caagcatatc cttatacttt aaacttcggg
    22741 gatttgtatg caacaccaaa taatggaaga tatagatgga ctggttctaa aacaatagaa
    22801 ataccaacta tatctacaac tggaagagta gattcaaaca gagatacaat agcagtagct
    22861 caaagaaact atgataatgc ttgggaacct aaggtattaa ctaatcaaag gaaatggtca
    22921 acgttggttc atccagcaga tataaaccaa actaattatg tggcttcaat aggcaatata
    22981 acaaaagtat ataatgagga acaaaagttt ccagagatgg atgcttactg tatatctaaa
    23041 atatatgctg attggaccgc attaggtaac acagcagata caactgttct tacaacaaca
    23101 aacgtattag aagtatttga taagttaatg gaaaaaatga cagaagctag agtacctgaa
    23161 aatggaagaa tattgtatgt tactccagta gtaaatacac ttatcaaaaa tgcaaaagag
    23221 atacaaagaa cagtaaacat aaaagatggt ggaacttctt taaatagaca aaccacagat
    23281 attgatacag ttaaaataat taaagttcca tctaacctca tgaaaactgc atatgatttt
    23341 acaactggat ggaaagtagg agcaggagct aaacaaatct ttatgtcctt agttcaccca
    23401 agtgcaataa ttacacctgt ttcttatcag ttctctaagt tagacgaacc aacagcagtt
    23461 acagaaggaa aatacttcta ctttgaagaa agttttgagg atgtatttat attaaataaa
    23521 aaagctgatg caatacaatt tgttgttgaa ggagctggag cataatggca caagtaagaa
    23581 aattaaatag aatattaact atagaagaat gtaaaataga tgatttctta gagatgggat
    23641 atgatttgat agatgaaact ggtaaggcag taaagtatgg caagtcatta aatgtaaaag
    23701 atttaatagc tgaaaataat attttaaggt caaaagtcga gtctttagaa gaagaaaata
    23761 agcagcttaa agagaaaaat aagcttacta aaaagtaggt gaaaattgtg gaaaataata
    23821 tgattgatga tatagaaaga agacttcaag gatttggata tgttctaaag gatggagata
    23881 aatggttaat agattttgta agagaaaaaa tagaaaatat tattaaacta gattgtaata
    23941 taagtgaaat tccaaaagag ttacacaata tagaaataga tatgatagct ggagagttct
    24001 tatttaccaa gaaaaatatg gggcaattag atatagaaag tattaacttt gaagctgtag
    24061 aaaagtctat atcagaaggt gatacaaagg tagattttgc tataggaagt ggctctcaaa
    24121 caccagaaca acgctttgat agcttagtag cttatcttac tacttatggt aagaataata
    24181 tattaacctt taggtgctta agatggtaag taaaactaga aaagcaatag aaatgttata
    24241 tagagataaa tgtactatag ttgagtatca gccaattaaa gaccctgtaa caaaacgaac
    24301 taacaataaa gaagtgattg tattagaaaa tcaaccatgt aaactttcat ataaaaatat
    24361 agtttctgct acagaaggga aagtagctaa gctagagcaa actattaaac tctttatatc
    24421 tccagatata gaaattaaag caggttcaaa acttattata aatgataaag agtatgtaag
    24481 aagtggagaa tcagctatat atccaaatca tcaagaaata atacttgagt tatttaagga
    24541 taaagcataa tggctagatg gggcagtgtt gattttagag agtttaaaag agtttgtaaa
    24601 aagatggaga agcttacaaa gattgattta gataagtttt gcaaagatgc agcaagagaa
    24661 ttagcagcaa gactcttagg aaaagtaatt agaaggacac cagttgatac aggattctta
    24721 agacaaggat ggaatggagt ggcttatgct aggtcgcttc ctgtgtataa acaaggaaat
    24781 aattatatta tagaggttgt taatccgact gaatatgcaa gttatgtaaa tttcgggcat
    24841 agaactaaag atggaaaagg ttgggttaaa ggacaacatt tcttaacaat ttcagagatg
    24901 gaactacaaa gccaagttga taagattata gagaaaaagt tattaatatt acttaaagga
    24961 gtatttgatg cttaataata ttatagatgg aatatcagta aagttagata aatcatttgg
    25021 agaaaaatat acaatttata gtgaggatgt agagcaaggt attaatgaac cttgtttttt
    25081 tattgttcct ttaaatccaa gcaagacacc atatccaagc gggagagaat taaagaaaaa
    25141 ttcttttgat gtacattatt tccctcgttc agaagctaag aattttgaaa taaatgagat
    25201 agctgagatg ctactggagg aattagagta tatagaaatt gatggagatt tagtcagagg
    25261 tacaaatatg aattttgaaa ttatagacaa tgttcttcac ttctttgttg attataacta
    25321 ttttactata aaaaataatg ataccaataa gatggataca gtagagttat tcggtggttt
    25381 gaagagaggt gataattttg aataaaacat taagcaaaga agatgactac aagtttacta
    25441 aggagcaaat agttaattct aagaagtatg taaatagaaa agacttatta aatgcaattt
    25501 taaaagaaaa tgagttatat tccttctcag aggtagagga aataataaat aaatttatga
    25561 aaggagtgag ctagatggcg ttaggtggag gaacatttgt aacacagaat aaaatattac
    25621 caggtagcta tataaatttt atctcagcta agagggcaac cagttcatta tcggatagag
    25681 gtattgttgc aataccttta gagttagatt ggggcataga tgaagacgta tttcaagtaa
    25741 ccagtgatga ttttgagaag tattcagtga agtatttcgg atatgattat actcatgaga
    25801 agctgaaagg tttgagagat ttattcaaaa atataaggtt gggatatttt tataaattaa
    25861 ataaaggcgt taaagccagt tgtactatag ccacagcaaa atatagtggt atcagaggaa
    25921 atgacttaaa agtaacagtt acaacaaata tagatgataa tgctaagttt gatgttgtaa
    25981 cacttttaga taataagaag gtagatactc aaatagctaa agtgattaca gacttacaag
    26041 ataatgacta tatcacttgg aagaaggatg caacactaga agcaagtgca gggctggtat
    26101 ttactggtgg aactaatggc gaagctgtga caggagcaga gtaccaagct ttcttggata
    26161 aaatagaaag ctatagcttt aatgctttag gatgtttggc tacaacaaca gagattaaaa
    26221 gtttatttgt agaatttaca aagagaatga gagataaggt aggagcaaag tttcaaacag
    26281 tactatataa gaaaagtgat gcagattatg aaggtgtagt gtctgtagaa aataagatta
    26341 aagataaaga tttagttgaa tctagcttga tttattgggc tactggagct atagcaggat
    26401 gcgatataaa taaatctaat actaataaaa agtatgatgg tgagtttgat gttggtgtaa
    26461 attacactca aatacaactt gaagaagcac taaagagtgg taaatttata tttcataaag
    26521 ttggtgatga agttcatgtg ttagaggaca taaatacttt tgtatcattt acagatgata
    26581 aaaatgacga tttttcaagt aaccaaagtg ttagagtact tgaccaaatt gctaatgata
    26641 tagcgacttt atttaataca aagtacttgg gtgaagtacc aaatgataaa tctggtcgta
    26701 tctcgttttg gaatgatgta gttaagcatc atgaacaact gcaaaatatg agagcaatag
    26761 aagatttcaa agctgatgat gtttctgtag aacctggaag cgacaagaag actgttgtag
    26821 taagtgatgc tgtaaaagtt attagtgcta tgagtaagct ttatatgact gtttcagtta
    26881 gttaacaata agaaaggaga ataataatgg cacaacaaat aaaagcaaga gatacaataa
    26941 gtgcatctaa ggcagagtgt tttgtaacta taaaaggtaa aagatataat tttatgcaag
    27001 ctattaactt agaagctaaa atggaaaaga ataagagtga gatacctata ttaggtagta
    27061 ctacaaaagg aaataaatca acaggaagta aatattcagg aaatgcaaca ttttattata
    27121 atacctctat atttagagaa ttgttgtatg agtataaaga aactggtgag gatatttact
    27181 tcgatataca aattaccaat gaagacccaa caagttcagt gggtcgtcaa actataatac
    27241 tggaagattg caatatggac tcaggcataa ttgctaaatt tgatgctgat ggggagtatt
    27301 tagatgaaga tatggatttt acttttgaga attggaaatt agttgagaaa tttaatatag
    27361 caaatggtat ggagtaaaat acacatttat gatttatgta tgtgtatttt ttatttataa
    27421 gaataggaga tgattaaaat taaggataaa tatgagataa aagattcaat ttcttttgat
    27481 tatagcaata aaagaccttt ggaagaacgt gtcagcgaga tgtataaaaa ggcaggaaaa
    27541 tatcttatag atatttcaga taagttagca acagatacaa ttgatggttc gtcattaaag
    27601 tcaataatca taaaatttga aataaatgaa gctggtgttg caacaataga aaaacaaaca
    27661 aaatatttgg tgatggaggt agaataagaa tatgggagat ttaaacgctt ttttaagtca
    27721 aaatgcaata aaagtagaga atagaaagta tgtggcaagt gaaaggttta taggagaaga
    27781 tggaaaagca atcgaatggg aacttaaagc aatagattca gatagggaca gacaattaag
    27841 aaaagattca actataagag tacctatatt aaataaaaaa gggaaagcta caggacaata
    27901 cactagtgaa acagatttta atacttacac tttgaaatta tgtgtggaaa ctatagtatt
    27961 tccggattta catgatgcag aattacaaaa tagttatggt gtaatgggtg cagaggagtt
    28021 attaacaaca atgttgactc ctggcgaata tacagacctt tcaagtgagg taggagaggt
    28081 aaatggtttt gatagaactt ttgaagataa agtagaagaa gcaaaaaact aatttatgaa
    28141 ggtgattatg attctaatat agctcattat tgccttcata aattcaaatg gaaaccacat
    28201 gaatatacag atttaccaga ctacgagagg gcatttgttg ctgcttctat agatattaaa
    28261 gtagaagaag aaataaaaga agaaaaaaag actgctaaag aagctagaag aagtagaaga
    28321 agataaaata taggtaaaat atgtaagaat tatatgttat aatattttta gcaagaagat
    28381 gtaatctaca atttatagag tggagttcat acaaaagatt atcctcccaa cgtatagaag
    28441 ggaggtgaat atgtatggat aattttttac aaaatgtact agaaagacta tctgttagtt
    28501 taatagtttt tctagttagc aatttactta aaaaacgtaa aaaaccactc aaagctggca
    28561 ctaagagtgg ttgggaattt gatttaaaaa tcagattcca taagttcaaa taagtattat
    28621 gaacttcact ctactgcaaa tagattgtag ttcttcttgt ttttattata ccacaaaaaa
    28681 ttataaataa aaccgttgtt tatataaaat atttgaaatg atagcacatt cccgttttgg
    28741 gaacatggtc aaaactcaaa ctatattact ggtattatat taaatattta gcatcaaaat
    28801 taaataaagc acttactttt tggtaggtgc ttttttatat tcaaatctgt gtacttaatt
    28861 gaaatatttg gtaaaatatg taagaattat atgatataat aattgtagca aggaaaataa
    28921 aattgaaaag ctaagggtga ttttctcata taaacgccaa attccaatat aggaaggagg
    28981 tgaatttata tgttaattaa ctttttattg agtatagtag ctggaattgt ttcagcttac
    29041 atttacgaga aaataaaaaa tcacccaaac gccaataagg gtgatttaaa gaaataactt
    29101 ttaaattcta atcaattaga gaaattcacc ttttacaaag taaattttat ctccttgcta
    29161 ttattatacc acaaattggt acagatattc aaaaataata tttttatgat atggtaaaaa
    29221 tatagaagta ttgtggtatt tcagcttcga gtaaattgtc taaaatgtta tagacggttt
    29281 tatattaact atatagtatg taaatattaa aatattttca tttacttatt cttaaataaa
    29341 ctaataataa gattaaatat aataaatcca gttgttgaac ctaaagcact aacaggtact
    29401 gatgatatat tagcgaaatt attcaatgct gatagacata acattactat agaaactata
    29461 actaaataag atatcactgt aaaaaatgga attgaaatac ttatatttaa ctctgcttca
    29521 tattctttca taatatcatt cctttcaaat tattttactt aactttatta tagcatttaa
    29581 acataattaa atgtaaaaat acagttaaaa attaattgaa aatatcaatt atatgttgtg
    29641 atataataaa attatagaag ttttgcagtg agcgatattt gtgataaaat atggcctaac
    29701 acttgaaata taatgtattg aggacgtatg ataagtatta tcatttgcac tactcatggt
    29761 tcactgcaaa tttgagagag gtgtgtatgt gtaagtcttg gaaatactca atttattttg
    29821 gggttttaga ttaactatat ggaatgtaaa ttattctaca gttgatacta tgcttaagcg
    29881 cgggttttag attaactata tggaatgtaa attttctcct aatttaggat taaacaaaga
    29941 ggttaaatag ttttagatta actatatgga atgtaaatat taatagaact ggtccgagtg
    30001 ctgctgctaa tcctggtttt agattaacta tatggaatgt aaatataaat gtatttcata
    30061 caggaatatt tttctgcaac agttttatat taacaatgta gataaaaatt aaataaagaa
    30121 aagaaagcac ttacaaacat gtaggtgctt ttgttttgct caaattggtc gattgggtaa
    30181 aataattaga aaaaattggg ataagttatt gacttttgag ttccaaaact atataattta
    30241 attatggaac tcaaaagtga ggtgataaca tgagtcctaa aaaaatagga agacctgttg
    30301 taggaagccc taaaactaat gatattaagg ttagagtaga tgatgaaact aatgaaaaat
    30361 tagatgaata ctgtaaaaaa aataacctta caaaagcaga ggctataaga cagggaatcc
    30421 atttactttt agaaaaataa caaaaataga gtgttcgccc gaccaagttt gaacactcta
    30481 tttaaaccat aagaagtaat cttctatatg aaatatttta tcatatagag aaacttcttt
    30541 caaattaaat ttaggaggag tatgtatgat gaatgattta caaatattta aaagtgaaga
    30601 ttttggagag ataagaacag ttgatattga taatgaaatt tggtttgttg gtaaagatat
    30661 agcagaagct ttgggatatt ctaatacaag agaagcttta aaaactcaca ttgataatga
    30721 tgatatagct gaggtcgtga ttcacgacgg cagccaaaat agaaatatga aaataaccaa
    30781 tgaaagtgga ctttattctc tgatatttgg gagcaagtta gagacagcta aaaattttaa
    30841 gaattgggtt acaaaagaag ttttaccagc tattcgacaa catggtgcat acataacaaa
    30901 taacgctgac cctcaagcat taagagaaaa agcaaatgaa atagaaagtt tagatacagt
    30961 taataagact atagaaatac taacaccttt tcttgataat gctggaatag atgaaaaagc
    31021 aaagttactt acagcaaaga ctatctacaa aaaagcagga atagagttgc ctttggagat
    31081 tgaagaaaaa gaacatttct ttgatacaaa acaaattgca actaaattaa atatgtattc
    31141 taagtctaat aaacctgcat ttcatgcaat aggtgaaatt attaagaagt tagatataca
    31201 agataatgaa aagttagtag tactagagag taaaagaggt tggagtggaa gtgtcaataa
    31261 gtattcacag agtgtaatag ataaaataag acattggata ggtgaaaaca ataagcctgc
    31321 taagatacaa ggtgagaaga agaattttca tgtggtttat aagattgagt aaatttataa
    31381 gattgtatta aataatttat tttagtttat tttgggggga ttaatacaat gattgaagat
    31441 ttacttaatg aatataattt aaaaactgat gaagatgtag agtattttgt gaagtttgct
    31501 aatgcattat atgaactaaa acagaatagt gaagaaaaat ttcaagaata tgcagagata
    31561 ttaagaggta ttcttagaga acaagaagag agaaaaaata agtaaaataa tatagataaa
    31621 gcacttgaat attatgatgt ttcaagtgct ttgtttgtta aaaaatgata taatataggt
    31681 aagagttata ttaacaatgt ggtatgtaaa gtgactttca tatgctcttt tatcagcttc
    31741 tgacataagt tttatattaa ctaagtggta tgtaaagact ggtaaaactc ttaatgtata
    31801 tgttatttgt aaagatttta tattaactat gtggtatgta aagcagcttt ttcttttaag
    31861 ctacataact gattatacta ttttatatta actaagtggt atgtaaagtt cataacttga
    31921 tttgattcat aacttgtagc cattgtttta tatcaactat gtggtatgta aattgtctta
    31981 ttttattatt aaagttttct tgttcatttg tgttttatat taactatgtg gtgtgtaaag
    32041 gaattagtag agtacaaaaa tttaaagtca gaatttagta ttatattaac taagtggtat
    32101 gtaaattcaa atataaaaga ctttacatct ttgccatcga ttggttttat attaacaatg
    32161 tggtatgtaa agaaggaact tttaagaaga atgttacttg caggagaaat tttatattaa
    32221 ctaagtggta tgtaaagtcc acatctgcat ctttacttat gttatttgta ttgtgtttta
    32281 tattaactaa gtggtatgta aagtcattta tcatgttcaa tctagcattc gtactagtca
    32341 gttttatatt aactaagtgg tatgtaaaga atggagctat agtagacagc attgatacaa
    32401 atattatttt atattaacta agtggtatgt aaagtttgca atagcttgtt tcataagata
    32461 tacgcattga gttttatatt aactatgtgg atttaaaatt aaaaataatg aaaaacactt
    32521 acttgaatag taggtgtttt tttattgaaa ggatgtgata ataatgtaaa aattttactg
    32581 atatagtata ataatcttat aaaattatgt agggggtaat attatgggat tatttggggg
    32641 taaagaacca tgcagcatat gtggaggaaa gggtaaaaat aaagttttag aaaatgaata
    32701 tttatgtaat aattgtttta ttaatttcac aatattttct agagaaagat taaaagcaac
    32761 tagtgcaatg caagtattag ctgaccatga agggataaga aaatttataa atttttctaa
    32821 aacaaataga gaactattag caaaatttgt tgagacaaat agaattaata aatttgtatc
    32881 cattgatgaa gataataact ttattaaaat atcagatatt cgcaaaggtg gagatgtaat
    32941 agagaatgta tatgcaattg atgaaattat gcaatttgaa cttttagaag atggagagtc
    33001 tatagttagt ggaggattgg gaagagctgt agttggagga gctttgtttg gtgctactgg
    33061 agctatagtt ggtggaataa caggcaaaaa aacaacaaga aaagttgttg atacatttaa
    33121 aattaaaata acctttaata atatcaataa tcctttggaa tatgttaatt tgattaatac
    33181 aaaaacaaaa actaattcat ctatatatca gaatgcttgt aatgaagcac aagaaatttt
    33241 atcagtactt tcaataataa taaaaaataa tgataagact gataatacac aaagtaaaac
    33301 tagtgattct atagaacagg ttaaaggttt aaaagagcta ctagacttag gagcaataac
    33361 agaagacgaa tttaatacta agaaaaaaga attgttgaat ttgtaatatt ataaaacact
    33421 tacttatgta ggtgtttttt tatataaaaa ttttagaaag gagagtgaaa atatggctac
    33481 aatacaaaca tcaattcgaa ttttcgatgg aatgacacct gcctttagga atatgactaa
    33541 ttctattaat acaacaatta atagtttaga gagactgcaa ggaagattaa ataatccact
    33601 taatacaggt ggtatacaag catctcagca gagcttaaat aatattgaaa atatcttaac
    33661 tagaatagaa cagaatatag gaaaagcaga tgaacaacag agaagattta acgaagatat
    33721 taataaaggg gtaagcaata cagatagatt gcttggaagt gttaaaaaga ttactggagc
    33781 ttatatgagc ttaaaaacat taggaggatt aggcagttta agcgaccaaa tgactaacac
    33841 taatgcccgc ttgagtctga taaacgatgg tcaactttct gacggagggt taaataaaat
    33901 gattttccaa tccgctgaaa ggtctcgtgc atcatatttg gatactgcac agatagtatc
    33961 acgaatcgga atgaatgcag gaagtgcatt tagtagtact agagaaatag taagttttgc
    34021 agaacaattg aacaaaaaat ttataatagc aggagctagt actcaagaga tgagttcggc
    34081 acttttacaa ttgactcaag gtttgggttc tggtgtgttg agaggagagg aactgaatgc
    34141 tgtatttgag tcagcaccta atataattaa gtcgattgct gactacttag acgtggatat
    34201 aggaaaaatt agaggaatgg caagcgaggg aatgttgaca gcagatattg taaaaaattc
    34261 tctgcttgca gcatcagcag aaacaaatgc tcaatttgaa aaaatgccac ttacaatagg
    34321 tcaaatcttt acatcaatta agaataacgc agtaatgata tttggagcga tacaaaaaaa
    34381 gattgaggat acagtctcaa gcggaggatt tcgaaccttc atagttaatg taacagattc
    34441 gttgtatgtc ttaggagctg ttggatatag tgtctttaat gggtttattg atttgctgag
    34501 cagtccaaca tttcaaggtt ttgcaaatat gatgattgtc ggaatcagtt taattgcgca
    34561 agggttggga tggataataa cacaagcact aagtgttgct aatgtattta cacaaaactg
    34621 gagtattatt gcacctatag tctatggtat tgtaggagct tttattgctt ataaagttgt
    34681 aatatattca gtttttttag ctcaggcaat ttatactgca attactttta cacaatgctt
    34741 tgtaacagca cttttcgctg gagaattata cgttgctaca aaagcaatgc ttatacacca
    34801 attgatgact ttaggagtaa gcagagctaa tgtcacacta tgtgcgacta tgattatggt
    34861 ggtatcagct gtagccttag taatagcagc gatattcgta ggagtagcta tattcaacca
    34921 cttcgcaaaa acgggtataa gtgcaactgg agtagtaatt ggagcttttt atttcttggg
    34981 agcttgtatt tatgacgtat tcgcaggagc ttggaacata gtaatggcat ttacggagtt
    35041 ctttgttaac tcatttaata tatgtatcta tggtgtacag atgctattct ataatcttgc
    35101 aaatttcgtt ataaactcaa tgggtgatgt tggaggttct ttcgatagat gtgcaacaac
    35161 acttgcaaat gtattcataa atggagtaaa tttagcaata aaagcagtaa atgggttaat
    35221 tggattaatt aataaaatac ctggagtaaa tatagatacc tttggtcatg gttatacaaa
    35281 aaatatagac tctgtcgttg gaagtttaaa aaactatcaa aaaacattaa aagagcctat
    35341 taaaccacaa gactggaaag caccatatat ggacattaaa aatccatttg actcttttaa
    35401 taaaggatat aaagtaggtc aaaatttaga aaacaaatta aaagatgctt ttgacatcaa
    35461 taaaatagca gaaaaggcta aaaaggattt aggactagat gacctttggg acaaaaaata
    35521 cggtcttggt gacggactcg gttcggcagg gcttaattct ccgctcagcg acgctgcaaa
    35581 aggagcaaaa gatacagcag gaaacacagc taaaatggca aagacaatgg ataaaagtca
    35641 agaggactta aaatacttaa gagatatagc tgaacaagaa gtaataaaca agtatacagg
    35701 agtcaacatt aaaattgaca tgaacaatac aaacaacata agtaaagata ctgacttaga
    35761 tggaatagtt aatgtattaa ctgaaaaatt aaacgacgcc atggttgtat cagcagaagg
    35821 aatagtttag aaaggaggga tataaatggc ttatgatttt tacttggatg gagtgcaatt
    35881 accaatcaca cctggcaaat tagagattaa agtaacaaac aaaaataaaa cagttgattt
    35941 aataaacact ggagaagtaa acatactaaa aaaagaaggg ctatctgaaa taagttttga
    36001 agcagagttt acacataata aattgccttt ttgtagaggt caatttagag atgttcaatt
    36061 ctttttaagt aaactggaat tactaaagac agattgtaag ccatttcaat ttattgtttc
    36121 aagggaatta ggtaataaag tactatttaa cactaatatg aaagtgtcat tagaagaata
    36181 taacatagta gaagatgcag agaatgcctc agatacaaaa gttgcaataa agttaaaaca
    36241 atatagagat tactcaacta aaaagttagt tcctgctcca cctaaaaaaa ctaatgagac
    36301 tggtagacca agcgtaaaga tagagccaaa acgagttgat tcagtcaatg caccaaatgc
    36361 taaaacatac acagtcaagg caggggatag tctttggtca atttgccaaa aacagttagg
    36421 taatggctca ctgtataaga aggtatatga gttaaataaa tcaatgatgg ataaggctaa
    36481 caagggcaaa aaagtaccta aatataccat ctataaaggg caggtgttga ggcttgtctg
    36541 atgaattagt tttggcaaat gatagggatg taagattagt catagcacat tgggaagatt
    36601 tctacgaacc tgtagttttg gatggtatca catgggaaat tgaaaggcga ggaacacctt
    36661 caaagttaga atttacaata gtcatggatg atatattaga gttttgcgaa ggtaactcag
    36721 taagattata ttacaaaggt gtaggcatat tttatggata tatatttcag aagaaaagag
    36781 ataaagaaaa tcacattaaa attgttgctt acgaccagct aagatatttt aagaataaag
    36841 atacttatgt atatagcaat aaaactgcaa gtgaacttgt aaaaatgttg gctaaagatt
    36901 ttaatttaaa atacaatgtc atagaagata ctaagtataa aatatctagg atagaagaaa
    36961 ataaaacact ctttgacatg atactaactg cacttgatga tactttaaga gagaaaaaag
    37021 aaatgtatat cttgtatgat gattttggaa gaataacatt aaagaatgtt gcatcaatga
    37081 aactggatac tgtcatgaac aatgatgtaa ttgaggattt tgactataat tcatcaatag
    37141 atagtgatac ttacacaaag attaaacttg tgagagataa tgaagaaaca gggaaaagag
    37201 atgtatatat tgcacaagac tctacacata tgaggagttg gggaatacta caactatttg
    37261 atacagtaga taaaaacatg agtgaagcag agataaagca aaaatgtgat atacttctaa
    37321 aactatataa taagaaaact aagtcattaa gtttaaaaaa tgtgttagga gatattagag
    37381 taagagcagg ttgtttagta cctgtttttt tagatttagg agatattgat ttacaaaatt
    37441 atatgttagt tgagaaagta aagcacacat ttgaaaataa ttcgcacttt atggatttga
    37501 ctcttgttga tggagatgaa tttgcttctt attcttcaag ctcatatagt agtggaaata
    37561 ctaataataa agatgaaaag aaaaatggtc ctgcacaaag tactacaagt aaagaagaca
    37621 atgatatgat aaataaatta aataaagtat ttaaaaataa gttatcaaat acaggaagta
    37681 tatttgttaa atattctaat gcttacaaag tcaatgcagc tttaatggct gctatttcta
    37741 tacatgaaac tggtaatgga agttcttcac tttgtaaaaa taaaaataat ttctttggaa
    37801 tgaaaggaat gtcttttggc tctgtggatg aaggaataaa aagaggtata agtaatttat
    37861 caagaaacta tatccatatg ggaagaaaaa cattagaaag tattagagac aaatatgctc
    37921 cactttatga tagtcctctt aataaacatt gggtaccagg agtaaataag ttttataaac
    37981 aaataacggg aaatgcatat agttctaata gtgcaggtac aggtgttaga agcaacgagg
    38041 aagcagaaaa gaatttaaaa gatataactt atcaagttca aaatagcaac aatgctaata
    38101 catcaacaaa caataatagt aaagtaaata aagttattca agaagcaaaa aatcaacttg
    38161 gcaagcctta caaatggggt ggtaatggtc caaagagttt tgactgtagt ggtcttatgg
    38221 tgtgggcatt caaaagaggt gcaggtataa atctcaaaag agtttcagca gaccaatcaa
    38281 atgatagtag aggaaaacta ttatgcaaca taaatgatgt aaaagctggc gatttagtat
    38341 tctttgcata caaccaagga aaaggaaatg tacatcacgt tggactatgt ataggaaatg
    38401 acaagttcat acattcacca aatactggtg atgtagttaa aataagcagt ttaagtggta
    38461 gacaaaaaca aaaacatgat tttgcaagag ctagaagatt cttttaagtg aggtgataat
    38521 atgagccaag atttattaca aataataaag aaagctgcaa tggatgcagt agaaacaagc
    38581 aacccaatgc agattgcatt tggaactata gaaagtgtta atcctttagt agttaagata
    38641 gagcaaaaag cctcttttga agaatctttt ctaatacaaa cagatacatt taaaagatat
    38701 acagataaaa agataggaga taaattagtc ttaattagga tgcaaggagg acagcaatat
    38761 ttgattttag ataggatgtg atgaaatgtt accaacagat aacattgact atgatataga
    38821 agatgtatcg ataattaatt ttgatgtaag gcaagaacca agtaagacct ttaaattaaa
    38881 tatagaaaaa tctaagatag atggtatttg tgatgatgtt gaagcattaa aacaaaccat
    38941 ctttttaatt ttaaacacag agagatacca acatctaata tatagttgga attatggagt
    39001 cgagttgaac gaccttattg gagagcctat atcctttgta atccccgaac ttgaaagacg
    39061 aatcaaagaa gcactaattc aagatgatag ggttgaaaat gtagataatt ttgagtttca
    39121 aaatgtaaag ggtaaagtac attgtaagtt cacagtttac agtaaatatg gaaatataaa
    39181 agcagagaag gtggtgagtg tataattgtt tgagttaatg acatttgaaa atataattaa
    39241 aagaatgtta gatagtgtac cagatacttt tgataaaagg gaaggttcta taatatataa
    39301 tgctcttgct cctgttgcta tagaacttac agaaacatac attgccatgg atgaattact
    39361 agaccaaact tttgtagata ctgctagtta ttactattta gagaagagat gtaaagaaag
    39421 aggtattaca cctttacctg caaccaatac aattgcaaaa ggagttttta atatagatat
    39481 tcctattgat tcaagattca acttaggaga atacaattat gtggcaattg agagaatatc
    39541 tgaaaaaaca tataaaatga aatgtgaaac tactggacct gtatttgaac ttggtcagtt
    39601 gattccaatt gaatatgtag acaaattaga aactgctgag ttaactgaaa tactaattaa
    39661 tggagaagat gaagaaagtg aagatagttt aagacaaaga tattatgata gcctaaactc
    39721 acagagtttt ggtggaaata tgcaaaatta taaagatgaa gttaacaaaa tacaagatgt
    39781 tggaggagtt aaggtttatc ctgtgtggga cggtggaggt actgttaagt tagtaataat
    39841 taattctaac ttcaaagttc catctagtga tttagttaat ttagttcaag aagaaattga
    39901 tcctctacaa aaccaaggag aaggtcttgg attagcacca attggacacc gagtcacagt
    39961 tgaaggagtt acaagtacaa ctataaatat atcagcagag ataacataca aaaatggcta
    40021 cacttgggag aatataaaat caattgcaga agaagcaata gacgactatt taaatgaact
    40081 taatatgagt tgggaagatg aagaaaactt aatagttcgt atatctcaaa ttgaaactag
    40141 attacttagt attgatggag tattagacat tgcaaataca atgataaata gcattgcatc
    40201 taatcttaat gttgataaag attctatagt attaagaggt gaagtagttg gataaagaga
    40261 ttaatctaat aaattactta ccgcaaatcc tacgagataa agaagaatat ataaaagtat
    40321 ttaatgtaga aaacaaagaa ataaaaacac tacatgaaaa attaaatgac ctatcaaatg
    40381 accagttttt agaggattta actccaagtg gcataaaaag atgggaaaag ataatgtcta
    40441 taactcctaa aagtaatgag agtttagaag atagaaggtt taggattttt agtaaatata
    40501 taagtaaatt accttactca gagagatttt taaggaactg gctagataat gtagttggag
    40561 aaggcaatta tgagttaact ataaataatg ctacttataa tatacatctt gaaagtgatg
    40621 ctagaaatca agattggttt gaggaagttc attcttttgt aagtaatatt aagccatgta
    40681 atatgacttt agattacact agagtgctta taagcaaaga caattatatg aattttggta
    40741 taacaaccct aatgggtcaa gaaataacta tatacccttg gagtccacca gatatagaaa
    40801 cttatggaga aattgatgta ttaactggca atggagttgg ataccaagag ataacaatat
    40861 tttaggaggt gatatattgg ctatagataa aagttattac actataatta cagatgtagg
    40921 aaaagcaaag atagcaaatg caagtgtcac aggtaataaa gtgggatttg taaaaattca
    40981 acttggtgat ggaggaggga gtgaatatac tccaactgag agtcagacag ctctcaaaaa
    41041 cgtggtatgg gaaggcaata ttggaaatac aactacagat gaaactgcac caaattgtat
    41101 aatattagag agtttaatac catcaagtgt aggcggattt atgataagag aaataggata
    41161 tttagatgat gaaaataatt taattgccat ttctaaatac aaagagtgtt ataaaccttc
    41221 tatagaacaa ggtgcagtgg tagacatgaa ggttaaaact gtgcttattg tatctaatgt
    41281 aaataatata gaacttaaaa ttgacccaac aataatcttt gcaacactca aagatataca
    41341 agacttagaa actaaaatag gtactgttaa tactaaaatt gatacaacta aaacagagtt
    41401 aaatactaga attgacacag aaaatgagaa acaaaatatt aaaattgacc aattaatcgc
    41461 aggtggttca aatgtatctc atacgcatat tatagagatt gatgattgga ttttaaataa
    41521 tgaaactaat atgtatgaag taactataaa tcatccacta ttgactaaaa gaatactaat
    41581 agctttatat gatgaaattg gtgaagcact tacaccaaac gctagggcta ttgatgataa
    41641 tagtattctt gttagaaatg aagaaaatat taaaatgtat gtatatttga taaatggaaa
    41701 tgcagaaact cattttataa atgcaactgt ggatgataac agagtgtctg aaatgactac
    41761 ttattcatct aagaaaatcg aagatagatt tcttaattta gaagaaaaag taaatggtgg
    41821 tttatctagt attgcaacaa gtgtaaatga gttgataact tattgttaga gaggagagtg
    41881 agaaaatgca gacagaatgg aactttgggt acaatggttc gccacaaagt gttatattga
    41941 aacctggcaa atataaattt gaatgctggg gttcttctgg aggtatcaac aattcttctt
    42001 ggcatactga tgctaaaggc ggatattcta aaggtgaaat tacattaaaa aaacaaacta
    42061 cattatatgt ttacgtcggc gaaagtggtt ttgcttcttc atctacgagt aataacacta
    42121 aaagtggttt taatggcggt ggtaaaggtt atttaaatca acaggttatg ggtacttatt
    42181 attctatgta cggtggtggt gctaccgata taagacttgt tggtggtgct tgggatgatg
    42241 agcaaggttt gctatctcgt ataattgtcg caggtggtgg cggtggttca tattctcctt
    42301 atactggtgg tgcaggagga ggattagcag gaggtactgg gtatagtgct aacgacagac
    42361 atcgtcccgg cggtactcaa tatcaaggtg gtattggtcg tgtaagcaca gaaaacggaa
    42421 gttttggaaa agggtgttct gctaaagatt caactggcga aggcggtgga ggtggctggt
    42481 ttggtggtgc aggaatgaat ggtgcgggag caggtggagg tggaagtggc tacgtattaa
    42541 ctaaagatag ttataagcct actggctaca caccaacatc tgaatattat tttgataatg
    42601 ttgttatgga atctggtgga aatactgctg gtgcttatgg ttatgctaaa ataacattac
    42661 tacaagcatt accattttta acagtatctt cttataattc cattacagct acatttaaa
//
LOCUS       DAMJVK010000028        42583 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.028, whole genome shotgun sequence.
ACCESSION   DAMJVK010000028 DAMJVK010000000
VERSION     DAMJVK010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 42583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42583
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.028"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            154..3702
                     /locus_tag="PC414_001548"
     CDS             154..3702
                     /locus_tag="PC414_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF2463946.1"
                     /translation="MMNRLKPRILGISILFIIIIILTCGAVFKFKFKLDNKLKETTHT
                     YLSEFTEHNIINLKTRLSGQFDMLESIANFIGNLNNINDEMVIDLMDSDVKRSSLIRM
                     TISTLDGECYSNDGITHNVQDRDYFKKAKNGEKNISEPLKSIVNNKEIIVLSTPVYKS
                     GKIVGVLSGIYDSKKLNELLGLSAFDEKADVFVAKKNGDIVAQKTNKNRKTLSCIFQL
                     FDKDNCDEDDCLKTIKENMKNDKSGYFNYNLDNEKFMLNYQPLGVNDWYVFSVVPNKI
                     VSIQSENISYDVYIFALEILLVILIFIIYIACIINNNTKIIINEKEKFKVLTDNILGG
                     VKTCLKDEYMTMTYVSQGFLELTGYSCEDLNKIFKNRFKDMIYKDDLDKFNCIILNQL
                     EKQDKIEVEYRIVRKDGSIIWILERSKSINDNKGSECIQGVLTDITELKKIQEELDSK
                     RFEIQAINDSIVGGIVITEINDDFDSIYLNEGYLNMIGYTRKQLEDELNNKLIKLIFK
                     DDRTELINSINKQLKLSDTFASEQRVEKRDGTIIWILLKGRKMIYDNGKSVLCCVIVD
                     ITSAKLLEQELKKSEERHEIILKQTQNIIIEWDIEKDKMNISPNWYNKFKYDPITENA
                     TKNIIKSVDIYHDDMDKFISLIKLTSQGSEYEECEIRLRTRDTIYAWFSIKATTLFDN
                     NGKPYKVVGSLTDIDESKREAEKLKERAEKDPLTDLYNKKASQSLIEEYITLDKKRKG
                     ALMIIDIDDFKGINDNLGHLYGDAVLSEIANDLINLFRATDIVGRIGGDEFIVFIKDI
                     SETRDIINKAEELVKTFERSFLGKGRNYKISLSVGIAKFPQDGMDYMTLFRNADIALY
                     SAKGKGKNCYTLYDKKFDKVQYITNTRNAGDSDNVEMSFKEHITNYIFDILYESSDIE
                     VAVNLILSIVGKHFNVSRMYIFENSDDNRVCKNTFEWCNEGVTSEINNLKNICYKDLG
                     NYLLNFNADGLFYCNDIENLGSDVKKVLKPQGIRSILQCKILEGNEFRGFIGFDECSY
                     NRMWTNDEVNTLTFISKTLSMFLLKMRIQSKLEESLTIQKSVMDNMDIWAYVVDRNTY
                     ELLFINKKMLESAPNTHVGDLCYKAIWNNQDKPCKKCPMIYLKDGDDTYKSFMHNKKF
                     EVQANITTTTLKWFDGKDACLIFGSEITSIEAEENT"
     gene            3887..5821
                     /locus_tag="PC414_001549"
     CDS             3887..5821
                     /locus_tag="PC414_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HDF2463947.1"
                     /translation="MENKFLPISKQDMIDRGWEELDFVLVTGDAYVDHHSFGTAIISR
                     VLESAGYKVGIIAQPDWRTTDDFMKLGKPRLAFLVNAGNMDSMVNHYSVSKKHREKDM
                     YSPGGKMGYRPDRATIVYCNKIREAYSDVAIVIGGIEASLRRFAHYDYWSDKVRKSML
                     IDSGADLLVYGMSEKQIVEVANALNDGYDPKYIRHIDGTCYISDTLEEIYDKYILIPS
                     YKEICEDKMKYVEAFKIQYDEQDPFRGNIIVQPHGSKYLVQNKPEKPLSREELDEVYG
                     LPYQKTYHPVYEKFGGIPAIEEVKFSIVSSRGCFGSCSFCAITFHQGRAVQSRSEKSI
                     IDEAIGITNLDDFKGYIHDVGGPTANFRRPACKKQITKGACKNRQCLSPSPCKNLDAD
                     HSEYLHLLRAVRKLPKIKKVFVRSGIRYDYVMADKNNKFLRELIEHHVSGQLKVAPEH
                     ISEEVLKYMQKPAGKTYDKFRQKFFAINEELGKKQYLIPYLMSSHPGSTLNSAIELAE
                     YLRDTHYQPEQVQDFYPTPGTLSTTMFYTGIDPLTMKPVYVPKSKRDKAMQRALLQYR
                     APRNYDLVYSALVEAGREDLIGFGHRCLIKPKNEKPYFNRNNSKKNVSKGTNKNKKTN
                     TNNRSNKNQQKSSTKKKKKH"
     gene            5927..6922
                     /locus_tag="PC414_001550"
     CDS             5927..6922
                     /locus_tag="PC414_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2463948.1"
                     /translation="MKKYISIILLVVLTMVVLVGCSPGKDNPKNKELSVVKQVKVAVP
                     DGLPAVAIAKLANENPEIKEGYETVYSIEKTPEAISTRVMKKDADIAIVPSNMAAIAY
                     NKTSSYNIVGTVGMGSLYLVSTENIKDYSDLVGKEVGCTGKGLTPDITIKSLLLQKDI
                     NYSDIKFNYVNSASELVPLLATGKVKTGIVPEPALSALMSKNPDIKIIKSLNDSWKEV
                     SGSKDGYPQSTLIVKSEFLRDNKDFVDSFVGQLSNSIDWANKNPEELGAYSEKIKIST
                     ESKIIGKSLERANLKYIPVKDMIKDYKNYYEKLANFDDKTLGGKVPDEAIYFVEK"
     gene            6894..7679
                     /locus_tag="PC414_001551"
     CDS             6894..7679
                     /locus_tag="PC414_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDF2463949.1"
                     /translation="MKQYTLLRNKKNKEQFIDKMEVTLSCVILLFLWQIIALKIDNDI
                     FLPTVGQVFNSIKEIMLNSSFYIDILYSMGRCFFSFLLAMIFAIILSIVSYLNRFFRN
                     LLKPINALTRSIPTMILVVLALIWFEKDSTPFIVGFTIVFPILYDNVLGAILNIDKNL
                     LEMANVYKIRFIDKVLKIYLPAIKFQIISILVSTFSLSLKVAIAGEVYSQPTYGIGTM
                     IQTEKINFNTSGIFAWIIIVLLISAILQIAQKFLARRAFLWKR"
     gene            7667..8365
                     /locus_tag="PC414_001552"
     CDS             7667..8365
                     /locus_tag="PC414_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2463950.1"
                     /translation="MEKIKLSIENINKRYDSRIIFRDFNIDFYVNEVNCILGKSGCGK
                     TTLLNIISGIIKNDTNNLNIKENLNRVGNKLEASYIFQDDRLIDWLTVEENIKIVVNK
                     YYNKTQLNKICDEYLELVGISDYKKFYPQMLSGGIRQRVNIARAFIYPSKNIIMDEPF
                     KSIDAKNTQLIMDNFRNILRKEKRTVLFVTHNIEEALFLADRIFILGDSPIRIKKILK
                     NSKELEKNEVLKLI"
     gene            8488..8961
                     /gene="ybaK"
                     /locus_tag="PC414_001553"
     CDS             8488..8961
                     /gene="ybaK"
                     /locus_tag="PC414_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HDF2463951.1"
                     /translation="MVKTNAMRILDSNKIDYKVMSYEVKSEHVDGVEVAHDIGRDVNE
                     VYKTLVTQGVSKNIYVYVIPVHENLDLKKAAKVAKEKSVEMIHVKDINKLTGYIRGGC
                     SPIGMKKFYKTFVNESAKNLDTIIVSAGKIGYQIELSPFDLQRLIKVEFVDVIKK"
     gene            9158..9997
                     /locus_tag="PC414_001554"
     CDS             9158..9997
                     /locus_tag="PC414_001554"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HDF2463952.1"
                     /translation="MRKIKSEQIVEQVKKLCIEASLYLGEDVLSCIKEKAKSEKSEVG
                     KNILNILVENAEIAKEKNIPICQDTGMAVFFVEIGQEVLIEGDTLTDAINEGVRQGYE
                     EGYLRKSVVSPINRVNTKDNTPAVIHYDMVKGDKIKIEFAAKGFGSENMSKMKMLKPS
                     DGLEGIKKFIIDTVSEAGPNPCPPMVIGVGIGGTVDKCAQIAKKALFRELGEFNKDEN
                     IAKLESELLTAINKLGIGPQGLGGTTTALGLNIETFPTHIAGLPVVVNINCHASRHKN
                     VVI"
     gene            10010..10558
                     /locus_tag="PC414_001555"
     CDS             10010..10558
                     /locus_tag="PC414_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HDF2463953.1"
                     /translation="MIKITTPVNEIDIAKLNCGDTISLSGILYTARDAAHKRLIDCIN
                     KGEELPFDVYGQGIYYVGPTPTKPGEVIGAAGPTTSYRMDDLTIPLLERGLRLMIGKG
                     KRSDEVIEGMQKYGAVYLAAIGGAGAYISNSIKSCEIIAYEDLGAEAIRKIVVEDLKL
                     TVAIDSYGNNIYEQGRAIYECK"
     gene            10624..11805
                     /locus_tag="PC414_001556"
     CDS             10624..11805
                     /locus_tag="PC414_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HDF2463954.1"
                     /translation="MSKDYAKLALEMHEINKGKVSVESKVEIKTKDDLSTAYTPGVAE
                     PCLKIHENQDDVYRYTSKGNLVAVVSDGSAVLGLGNIGAEASIPVMEGKAILFKQFAD
                     VDAFPICLKTNDVDEIVKSVELMEPVFGGINLEDISSPRCFEIEERLKKSLSIPVFHD
                     DQHGTAIIVAAAIINSIKLIENKKIEDLEIVINGAGAAGIAIAKILLNMNVKNIILCD
                     RSGALEASIENLNYVQKEMLKVTNIRNEKGPLKDIIKGKDVFIGVSGPGAVTKEMVES
                     MSEKPIILAMANPTPEIMPEEAKLGGAFIMGTGRSDFPNQVNNVLAFPGIFRGALDVK
                     AKEINEEMKIAAAYAIANTISDEEICPEYILPDVFNKNVVKNVAKAVKEAAIKTKVNR
                     I"
     gene            12100..12486
                     /locus_tag="PC414_001557"
     CDS             12100..12486
                     /locus_tag="PC414_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009248914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2463955.1"
                     /translation="MEYKVNDQELNASMFIPFVNRIWPGDYDMDKTQTALSKTLNITA
                     YDDKVLVGCLRVLSDGYYFGTITELLVLPEYQKQGIGSKLFQLAKENTPTMLYFGAQP
                     GVEAFYEKNGCQRSLQSYIIKKKDSY"
     gene            12636..12899
                     /locus_tag="PC414_001558"
     CDS             12636..12899
                     /locus_tag="PC414_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF2463956.1"
                     /translation="MEETKFCQCCAMPMGQTDELYGTNKDGSKSTDYCSYCFKDGEFT
                     ADISMEEMIEVCIPHMLQSNKDMTEDEARTMMNNFFPTLKRWK"
     gene            complement(13028..13870)
                     /locus_tag="PC414_001559"
     CDS             complement(13028..13870)
                     /locus_tag="PC414_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDF2463957.1"
                     /translation="MINIGDFNKLTVKRKTEFGYFLDGQTNNTKDDILLHNRLIGKNE
                     INIGDEVNAFIFKDSDDRTAATLIPPLAKVGDVAHLKVVDNTDIGTFIDMGLPKDILV
                     PFKAKTYPLFRDEKYLFYIYLDKSKRIAATTDIDSYLLTDHTYNVGDIVTGVVYGFQT
                     NNSAMICVDNKYAGVILHNEYFTELKTGDVLENLHVIKIYEDGKLGLSPRGNRKDELD
                     TLENKILSYLEGSDGYMRFNDKSDPKDISILFNSSKKNFKRALGVLMKKGLIYQDEEG
                     TYLK"
     gene            14165..14899
                     /locus_tag="PC414_001560"
     CDS             14165..14899
                     /locus_tag="PC414_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2463958.1"
                     /translation="MKIVNKSSNIPLHTQLSSIIREMIETGELKEGDAIMPERELCNI
                     QNVSRMTVNKTIVGLVTEGLLYRVQGKGTFVAKQKKKYQFSNVKGFTDVMKEKGVNIK
                     TDILSFEMELPDDLVKRKLGISDNTTNIYKIVRLRYTDGEPFGLEIVYLSEEMCKGLT
                     KGILDNSSLYRVLNEKYGYKIQKAEQVMEPVILSDEESKLLETDEGALALKLHRNSYN
                     REGSPIEYTISIFRTDKYQYEIVLSE"
     gene            14909..16042
                     /gene="nagA"
                     /locus_tag="PC414_001561"
     CDS             14909..16042
                     /gene="nagA"
                     /locus_tag="PC414_001561"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDF2463959.1"
                     /translation="MKCLINGKIILKNQILENKVLVFDEKIIDIADSVPKDCEVIDAD
                     GKYISPGLIDIHIHGNMGKDTMDSTDESIETISKSIMRHGVTSFLPTTMTMDKEHVYD
                     ALEVIKKAQNRKLEGAQVLGAHLEGPFINENYKGAQNEKFIINSKYEFIKEYKDVIKV
                     ITYAPEKDIDFDFTREIKRCTDIVLSIGHSNANYDQAKEAINLGVTNVTHMFNAMTGL
                     NHRDPGVVGAALTTNVYSELIADTIHINKDLFQFILNNKGKERLILITDSIEAGGLED
                     GNYSLGGQKVIVKGNEARLENGALAGSVLSLNKMVFNFLDNTNLKVNEAINLASLNPA
                     TSLGINDKKGSLEIGKDADIAVFDENLDCKMTLCLGDVVYKNI"
     gene            16141..16890
                     /gene="nagB"
                     /locus_tag="PC414_001562"
     CDS             16141..16890
                     /gene="nagB"
                     /locus_tag="PC414_001562"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012425552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDF2463960.1"
                     /translation="MRVLVCKDYDGMSKKAAEMIAAQIVLKPNSILGLATGSTPVGMY
                     RDLVKKYNDNIVDFSDVMSFNLDEYYKLPISNDQSYDYFMKENLFNHVNIKPENTHLP
                     NGMADDIEKECMNYEASIDAAGGIDVQVLGIGRNAHIGFNEPDTKFAKRTHVVELTES
                     TIEANARFFKSREDVPKKAVSMGIGSILKSKKILLLASGEEKADAVYNTVYGDITPEV
                     PGSILQLHKDTIVIVDEAAASKLNPKDYKLV"
     gene            17065..17337
                     /locus_tag="PC414_001563"
     CDS             17065..17337
                     /locus_tag="PC414_001563"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463961.1"
                     /translation="MLKIIMFSGILSSIIKLPIIGILPTGNIKKNTGISDSKKDGKQR
                     RSNLGISIYQLPSFLYSLINNSVPSNKPIPPEEIKLIKMYLLKSCI"
     gene            17997..18701
                     /locus_tag="PC414_001564"
     CDS             17997..18701
                     /locus_tag="PC414_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2463962.1"
                     /translation="MSKLIYIADDEDNIRNLVKTFLKNEGHDVMDFKTGDELLEQFNI
                     KECDLVILDIMMPGSSGFEVCTKLREKSTVPIIMLTARDTDIDYITGITLGSDDYFTK
                     PFSPMSLVMRVKSIFRRIEFEKKQNYDKYSNSIDMELKFGDVIINKKNKIVTSKNVNI
                     DLTPNEYNLLTYLFENIDRAVSRDELLNKIWGYDIEVETRAADDTVKRLRKKILNTNI
                     LIETVWGFGFRLKEKS"
     gene            18703..20190
                     /locus_tag="PC414_001565"
     CDS             18703..20190
                     /locus_tag="PC414_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2463963.1"
                     /translation="MKNGNDKNRIGKIKSKFKLKFSKKNISIKTRILLSILCLIIIVF
                     MIILVSFNVFLDTYIKTTANEELTKSTEIVEHMDSNFRPIKPPQDDGKLPPKVLSNFM
                     RNVQDKVRMAETQSDADAMVVDSKYNLIFPTREDDFLKNVDEMELIRECIQNEKLDLN
                     SDENTRISTRNKDYYISTVKITSIYGEQDKYLILFIDISKTLNLAQKINIVLISVMCF
                     AGILAIFTAVILSEKIAKPIKELCEFVKKMGQGDFKRTYFDFSDKELVELSTVMNKSA
                     EYLDKYDNEQKIFFQNASHELRTPLMSIKGYAEAIKYNVIDSKHASDIILEESDRLSD
                     MVEDLLYISKIDNITKDFELVECDLREVLSNCGARQNVRAINKGIKFIYEFDEEMVLF
                     ECDEKNISKAFMNLIENALRYAKSEIKIVCKYNQKNIVVIIEDDGIGIKKEDLPHVFE
                     RFYKGVGGNHGIGLSIVKSIVNKHGGRIYVENGKKGAKFTILFKL"
     gene            20395..22008
                     /locus_tag="PC414_001566"
     CDS             20395..22008
                     /locus_tag="PC414_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF2463964.1"
                     /translation="MKEQLIFNITNLVTVVFEAFVIHMFLSDFLGQKEGYIKVVRYAK
                     LGFIICLGFCNLITLNPKITMPLIFILIFSTSFLYKGNLKTRLFTTVLLSIFFILSEI
                     VVTSIFVLFVKEGFEIMLENNSIRVLATILSKIVFLLTCKIICLFKKDVHLDMPIKYW
                     LPLFLIPIFSLFLSVSIFDVSKFFSLESLKFLSLISSVGILYINFIVFYLFKFIIDKT
                     KLSMKYELLEKEIIHKEELRLSNECYKIIVEQTDSVVFEWNIKENKSFVSQAWTEKFG
                     YNNACKNIFKEIKDKDLVHSEDKAIFEGFLESIKKKNMHNQAVYRLKKSNGEYIWCRT
                     SITSIYNDENELLRVVGVIVDVDSDIKKYEELRTRAESDSLTNIYNKGTFEKLVEETI
                     VMNTGDKKDALFIIDLDDFKEINDNFGHPFGDFVLKTFADKIQTSFGSKDLVGRIGGD
                     EFVVYMQDYVTEVNLHKKAKELNRVLSDNYTDLSFSFDASVSIGIARYPQDGTSFFEL
                     FKNADRALYSIKASGKNSYCLFEEELYVQ"
     gene            complement(22371..23201)
                     /locus_tag="PC414_001567"
     CDS             complement(22371..23201)
                     /locus_tag="PC414_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2463965.1"
                     /translation="MSQVSKNYFTTGEFAKICGINKKTLFHYDDIGLFSPELKKENGY
                     RYYSYHQLSIFGIISSLREVKMPLKEIKAYIDKRTPNLLIELLEKKTIDIKNEIEKLN
                     NIQALMESTISFTKSACNIDANTITLKEHEEEYLVKTPVTYKEQFLGDEEENFLYECI
                     NFMDNYELSDYGTIGSIIKGEDIINKDFESYSYLFTKVNKEYKKYPVSIKPKGLYVTA
                     YHKGSYETIYKAYEKLLNFFNQNNLRIGDFVYEEYLLYDISVRDSNEYLTQISAEVKM
                     "
     gene            23274..25001
                     /locus_tag="PC414_001568"
     CDS             23274..25001
                     /locus_tag="PC414_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2463966.1"
                     /translation="MKKLIHFLKPYRVLIVVVLIFTFLQTLGTLYIPTLTANIVNNGV
                     VKGDIDYIVKTGLMMMIVAGITALSAVLVCKVSANLSSGFCRDIREAVFIKSQDLSIN
                     DFNNIGTASMITRSTSDITLIGQSVFMFIQLVLPAPIITVSGLFLAYSIDKAMTIIIV
                     VVMFLFMLSAFLVGKKLIKLFKMMQIKMDNMNRVLREVVTGVRVIRAFNRSRFEKKRF
                     DRTAIDYSETAISINKIFAVLMPIVMLIMNLGIVSIIWFGGMRVSNGNMEIGHIMALV
                     EYCILILFYLIMGVMVFMYIPRAGACADRVNQILDIEPEIVDGNGHKDTVSERGHLVF
                     KNVTFSYAQSEEPVLNNITFEAKSGEVTAIIGSTGSGKSTIANIIPRFFEIQSGEISI
                     NGQDIKKIPQKELRDKIGFVPQKAFLFSGTIEENIRYGKEDASIEEVKHAASIAQADE
                     FIYDMEDKYDSFVAQGGNNLSGGQKQRISIARALVRKPEVYVFDDSFSALDFKTDKRL
                     RKALKNEIKDSSAIIIAQRISTIMDANQIIVLNDGKIVGIGKHKDLLENCEVYKQIAD
                     SQLSKEELA"
     gene            25038..26900
                     /locus_tag="PC414_001569"
     CDS             25038..26900
                     /locus_tag="PC414_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2463967.1"
                     /translation="MSEKRIDEQDIEVLSFEDGMSAEKAKDAKKTTKRLLKYMAKQKF
                     KILIIFISVLISSALTVLAPMVMGKAIDQLFNGIKTAVQTGTKFSVNFSTMGGIVSIL
                     LGLYLISAVFIYIQQYIMSGVAQNLVLSMRKDLSDKLNKLPLKYYDSHKKGETLSIVT
                     NDLEKVADSLQEGLMQLITAVVTVVGSIVMMISISVPLTIVSAITLLVSLGITVVIAR
                     KSQERFSENQKALGELNSNIEEIFTGQIVVKAFSKEKDTIRNFKILNQNLYNASRKAQ
                     FSSYAISPIIRFINLIGYVIIAVVGGIFASTGAMTLGSIQAFIQYVNQASEPTTEISY
                     IVNMLQAAIASAERVFTVMDEVEEIKDEESSKVISMPKGKVQFEHVKFGYSDDFILMK
                     DININLNAGDKIAIVGPTGAGKTTLVNLLMRFYEIQGGRITIDGVNIKDLKRGELRTM
                     FGMVLQDTWLFNGSIKENIAYSKSDATMDEIVRAAKSARVDHFIRTLPQGYDTILTED
                     ASNLSQGQKQLLTIARAILSDPSVLILDEATSSVDTRTEVEIQKAMNNLMKGRTSFVI
                     AHRLSTIRDADLILVMKEGTIIEQGSHKELIEKKGFYEELYNSQFTSEYDEDVV"
     gene            27233..27922
                     /locus_tag="PC414_001570"
     CDS             27233..27922
                     /locus_tag="PC414_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HDF2463968.1"
                     /translation="MNKKLNYRFEMKHKITEADVLALKSRLYPIMKKDENASKDGKYL
                     IRSLYFDTPEDKALLDKLNGVAIREKFRIRFYNNDCSYIRLEKKIKHYNMTSKLSTNL
                     TKKEVINILNNDIEFLKESTNCLLREFYLKLKCERLEAKSIVDYNREAFIYPVGNVRV
                     TIDSDIKTSVNSVDLFNKDLPTISVIDENMTVLEVKYDEFIPDFIKDLIQINKTTSTA
                     VSKYASSRLYI"
     gene            28027..28713
                     /locus_tag="PC414_001571"
     CDS             28027..28713
                     /locus_tag="PC414_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HDF2463969.1"
                     /translation="MILIMTFNDIFKSSFIENVSGFSFVDSALALGSAFLVGLFIYMV
                     YKKTYMRIMYSRPFNVSLVALTMLTTFVILAVTSNVVLSLGMVGALSIVRFRTAIKDP
                     MDLVFLFWSLGSGIVLGAGLIPLAVMGSIIMGLILIFFSNKTISETPYILMVNCNNED
                     SEDIATDKIKKVFTKYQIKSKSVTPEKGIELVFEVRMKDGETSLINDLSQVDGVTNAV
                     LVSYNGDYVA"
     gene            28730..30637
                     /locus_tag="PC414_001572"
     CDS             28730..30637
                     /locus_tag="PC414_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HDF2463970.1"
                     /translation="MKDKKFTLLISIMIIFLCAVVGVYSTSSNKSADLYSDVYIEKYF
                     NRDKVMEVNIEIDESDLKDMNENAIKEEFKVAKVTVDGDTYGNVGIRTKGNSSLISVA
                     NSDSDRYSYKINFDKYNTSQSMEGLTQLNLNNCYSDPSYMREFLTYSICEEMGLATPE
                     FAYAKVSINGEYHGLYLAVEGLKESYLENNFGNVTGDLYKSDEGSSLQYKGDDPESYS
                     NLIVESDKKTADWSKITKLLKSLDTGEDIEKYLDVDSVLKNIAINTALLNLDSYQGSF
                     AHNYYLYEQDGVFSMLPWDFNMSFGGFSGFGGGSQSIAIDEPTTGNLEDRPLISSLLK
                     NETYKTKYHKYLEEIVTKYLDSDYLENMTTKLHDMIASYVKEDPTAFYTYEEFEKNIT
                     SSIEDSSDNKGFGNKGFDNNNSNNSDSNNNSNSENKRSGNQSDEKEVNAELTSSVVKA
                     NTDNETKNKTTNDSESKNNTDKDKSGNDNNQKLEGPMGKGGKSIPGVLEVAEDMSKTI
                     KSQLSGETSSTKQNSGDESSSGIKGSEKFDEDMSGMPEPPEGMDGKMPPGMGNMDKGD
                     MNGKNGNMNMDRNQDNPREAGGFGNRGGGSVSKTTTYFKLILGGASMIIMSIMLVGVS
                     RVKRRRFIKSK"
     gene            31090..31779
                     /locus_tag="PC414_001573"
     CDS             31090..31779
                     /locus_tag="PC414_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463971.1"
                     /translation="MLQKVLWKFLKLVVGLFICSVGIVLTINCNLGLSPWDVFHQGLS
                     NHIGITIGTASIIVGSIVVIADVVLGENVGWGTVFNMLLIGFFMDLLLYSNLIPEADS
                     LFVGIIMLILGLVLLSVGMVFYMGSGLGSGPRDGLMVAIQKKTGKSLKLIRGTIEVGA
                     LIVGFLLGGKVGIGTIISAFGLGYFTQMVFGLFKLDCSKIKHRFIVDDIKFIKVYMSG
                     EKKAAVQNIVK"
     gene            32215..32754
                     /locus_tag="PC414_001574"
     CDS             32215..32754
                     /locus_tag="PC414_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2463972.1"
                     /translation="MDIGEKIKRLRTEKQLTQEELANRCELSKGFISQLENNLTSPSI
                     ATLIDILEILGTNLREFFNEIDDERISFTKEDMFETEDEDLKYKLKWLIPNSQKNEME
                     PIIITLYPGGQYKEEKPHEGEEFGYVLAGSIYVHIGEKKNKVKKGESFYFRPKANHYI
                     SNEGKTTAKVIWVSTPPSF"
     gene            32773..33816
                     /locus_tag="PC414_001575"
     CDS             32773..33816
                     /locus_tag="PC414_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2463973.1"
                     /translation="MVENIIELRDISKHYEELTVLDNFNLDIKKNEFLTLLGPSGCGK
                     TTTLKIIAGFEYADDGKVLFEGKEINNLPPYERQVNTVFQKYALFPHMDVYENVAFGL
                     KIKKTPKDVIDAKVKEVLKLVALEGFERRQVESLSGGQQQRIAIARALVNEPKVLLLD
                     EPLGALDLKLRQEMQIELKRIQKKLGITFVFVTHDQEEALTMSDTIVVMYKGKIQQMG
                     TPQDIYNEPKNSFVAKFIGESNIFDGIMIEDYKVNFCNRDFECVDKGFEKNENIEVVI
                     RPEDIKMVKPEEGMLKGKVTSTVFKGVHYEIELNENGRLWILHNTKNAEVGTELGMDI
                     YPEDIHIMRKENN"
     gene            33818..34654
                     /locus_tag="PC414_001576"
     CDS             33818..34654
                     /locus_tag="PC414_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2463974.1"
                     /translation="MKRKSFLAYPYVVWSAIFVIIPLILVVFFSFTKESGGGYAFTLE
                     NYKEVIDPIYMKVFGRSILLAGGATLICLIVGYPVAYIISKARVSRRGSLILLFILPM
                     WMNFLLRTYAWVAILGKNGLLNTFLGWFGIQPLAILYTNFAILLGMVYNFLPFMVLPI
                     YTALSKMDNDLINAAHDLGANNMTVFRKIIFPLSLPGVMSGITMVFMPAVTTFAISRL
                     LGGGKIMLVGDLIEQQFTVVGDWNFGSAISIFMMIVILISMSIMSKFGDESDKEGGGL
                     LF"
     gene            34759..35433
                     /locus_tag="PC414_001577"
     CDS             34759..35433
                     /locus_tag="PC414_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2463975.1"
                     /translation="MARWNGFTWKWYGQLLQNESIMSALYYTIVIAILASVISTIVGT
                     ISAIGIHKMRGKSKKLILNVNYLPILNTEIVTAVALMSLFVFVKMEFGFTTMLLAHIM
                     FCLPYVILSVLPKMKQLPDNIEDAAMDLGATPIYALRKVILPQIKPGIVSGFLIAFTM
                     SIDDFIISFFNAGNGVSNLSIEIYGMARRGIKPEINALSTIMFAVVLGLLLLANKKES
                     IVRGIK"
     gene            35433..36485
                     /locus_tag="PC414_001578"
     CDS             35433..36485
                     /locus_tag="PC414_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2463976.1"
                     /translation="MIKFKKAISLIAVGMMTVTLFTGCNKATDSTKVLNVYNVGDYID
                     ESLIDKFEKETGIDVQYSTYDTNEMMYQKVKSGSTHYDLVFPSDYMVEKMKNEGLLEK
                     LDFKNIPNMKYLDKSFLNPVYDKTNEYSVAYMWGTLGILYNKKEVKDPMDSWNILWNP
                     KYKGNIMMFDSVRDTIGITLKKLGYSMNSVNPKEINEAKNLLMKQKDLVLAYVNDEGK
                     DRLLGGEVAMGMLYSGDAVTLMEQNPDLDYAIPKEGTNKWVDAMCVPTTAQNKKEAEL
                     FINFLLDPENAKVNAEYIGYSTPNTGALKLLDPEITQNPVAYPSKEVLDKCETFEDIG
                     ENIKLYDKAWIELKSK"
     gene            36715..37563
                     /locus_tag="PC414_001579"
     CDS             36715..37563
                     /locus_tag="PC414_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2463977.1"
                     /translation="MNRINKKIDLLILALLVFIFLIVGIVILSIRTENKLNVFIMLAI
                     IFFIIMLTYLSNSVVGLITSSIIIFMYTSYILYNNITHNMDVEFISYMWIIATPVSSI
                     IMGNLNKSINELQNTNKKLSEQYKELVTIDSETGLRNLKIFYNDVNMEISKSIRHNTD
                     FSLMIVKLPYYGNLQTIFGENKTNKIVKYIGSNIIECTRNEDIIYSLQKDMIGILMPN
                     TSLEGSKVVKDRIKKRIKELNLDLNNRGKYVNIDVKIAFLQYKSSFGDSINFKNIVEE
                     ELQYDV"
     gene            37553..39217
                     /locus_tag="PC414_001580"
     CDS             37553..39217
                     /locus_tag="PC414_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2463978.1"
                     /translation="MMFKIFNKSLLKLVVVLSIVLTISNFSLSSYASEITSSTDKVLI
                     IYDSKKETAYNRDILNIMRTLLGRFSSDIELLKLSNYDGEINKNYYSHIFILGINENS
                     YNNDKNTKNLISSLNLYKGTICWLGYGIENLLEHKKYNLDYVGKTNNIVSVNYRGKSY
                     NLDEHYVFNIVESKDTSNKVIGSINDTLNKYPYIINDKNLFYVSKLDLDGVLFYIFCD
                     SLNDIFNIKTFDKGRIFVRIEDVHAFREPKNLVEIADYLSSKNIPFTIALIPAYVNPK
                     NHKVITLSESPEIVKAIKYMQDKGGTVILHGYTHQYKKEEVSGEGYEFWDGKKDEPLK
                     ENMKIFVKDRVLNGLRVCIENGIYPLAFEAPHYAMESEGYKELKKYFSTYIGQHQNND
                     KKFSTNTYPYIIRDTEEFNIFIPENLGYIDPEDKFTFQNIKENLDKLSIVRGFSGGFF
                     FHSYLNIEYLKNTIEYLEKQNIEFMNLRDFNNWVKVDEIQIRNNGDEIIVNYDKDLDE
                     LTKSDTRFKSISNISKVLIFIVSISVLIFVIIFIYFKRIDKKKFLK"
     gene            39266..40522
                     /locus_tag="PC414_001581"
     CDS             39266..40522
                     /locus_tag="PC414_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003362983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2463979.1"
                     /translation="MRDLNVSEYLFVFSLFSIWSLLLINIILAMGGYIFYFKNFDKEI
                     KEIDEYPMISILVPAHNEAKVIGRTVESLLLLNYPKSKMELIVINDNSSDNSKEILEN
                     IKDRYNNYNFTIINTDSLTGGKGKSNALNIGYTISKGDFIAVYDADNTPDKNALRYLV
                     QTIVMNDELGAVIGKFRTRNKNKNLLTKFINIETLSFQWMSQAGRWQLFNLCTIPGTN
                     FILRRSIIEEIGGWDSKAIAEDTEISFRIYKLGYKIKLVPQSITWEQEPETVKVWIKQ
                     RTRWAKGNIYVLMKYIKNIFKQGRNKIVFDIAYFFSVYFLFLTSVIISDILFVLSISK
                     LVEISIPINFFLIWILSYLLFIIEVSISLTIEKGEATIENIFIVAIMYFTYSQLWLFV
                     AIKGMIEYLKDIIFKREVKWYKTERF"
     gene            40545..>42583
                     /locus_tag="PC414_001582"
     CDS             40545..>42583
                     /locus_tag="PC414_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HDF2463980.1"
                     /translation="MKKFIISIISLVLFFSNISLIYKVNADETKVKNYKFERDITIDG
                     VIGSNSTFFEVNKNWDIEEVLLHLNFSKSQILNGDVSSLTVLINNVPIKSIKLNAKTN
                     YKNTLEVLVPKDYIIQGYNEIKIKTYKTISDKICQDDSNTGNWMVIHKESYISIRYKQ
                     KKVENSINEYPYPYAEIENNHKLDTTIVVPDNMTRGETTAVFNLASAFGKITKNDDLK
                     LDVKLYSEMKNWSDDNIIYIGKPENTAEEILDILSIKEQTLLSSNCIIKQVDSPYNKN
                     KKMMVVIGSNEDDLIKASNLLIENRLSNQVLSSSVLVNKETNIKINREQKLNLGHLTL
                     KDLGYSDFLLEGAFNQQALFDVKIPTGKVLDDGSKIILNLRYSDNLDFEKSLVTVSIN
                     DVIVGSKKLDRSHSNNDKLELKIPKDIDNKNYYQVKLTFNLSIKNSNCVTRESNNPWA
                     YVSNNSYLALSTKENETLSFENYPYPFVRDDEFNDLTVIMPDYSGSQAMTWMFRLGVT
                     LGANINSHNGNINVIRGKEFSDKYKDTNIVVFGVPHNNSVIKMLNNNLNIKFDKNYSN
                     FISNDKISFIDDYGKNISTIQLIKSPYNNQKNIMVISSMNEKNLYLGMDYLLNKSKVN
                     DLKGDTLIIDEYGEVEDLAYNLKSKKEVKDSSWNMSINKTTKVFLMISFITIIIV"
BASE COUNT        16905 a         4340 c         7383 g        13955 t
ORIGIN      
        1 ttacaaataa atcttaagct atcttattta attctaatac aggaagaagg taaatgtata
       61 ttgaaaaaat catagaaata gaagtaaaca atttaaataa atacaaaggt ttgcaaaaga
      121 agtaatataa ttatatacaa tgaggatgtg agtatgatga acagactaaa gccaagaatt
      181 ttaggtatta gtatcttgtt tattattata attattttaa catgtggagc tgttttcaag
      241 tttaaattta aattagataa taaattaaaa gaaaccacac atacatattt gtctgagttt
      301 acagaacaca atataattaa tctcaagaca agacttagtg gtcaatttga catgcttgaa
      361 tctattgcta acttcatagg aaacttgaat aatataaatg atgaaatggt gattgattta
      421 atggattctg atgtaaagcg aagttcttta attagaatga ctatctctac attagatggg
      481 gaatgttatt ctaatgatgg aattacccat aatgttcaag atagagatta ttttaaaaaa
      541 gctaaaaatg gagaaaagaa tatatcagag cctttaaaat ctattgttaa taataaagaa
      601 ataatagttt tatctacccc agtttataaa agtgggaaaa tagttggtgt tttaagtgga
      661 atttatgatt caaaaaagtt aaatgagtta ttaggattat ctgcatttga tgaaaaagca
      721 gatgtttttg ttgcaaagaa aaatggagat atagttgctc aaaaaacaaa taaaaataga
      781 aagacgctat catgtatatt tcagcttttt gacaaagata attgtgatga agatgattgt
      841 ctaaaaacta taaaagaaaa tatgaaaaat gataagtctg gatattttaa ttataactta
      901 gataatgaaa aatttatgtt aaactatcag ccacttggag ttaatgattg gtatgttttt
      961 tctgttgtgc caaataaaat agtatctatt cagtcagaga atatatctta tgatgtatat
     1021 atttttgcat tagaaatact actagttata ttgattttta taatatatat agcatgtatt
     1081 ataaataata acaccaaaat tattataaat gaaaaagaaa aatttaaagt actcactgat
     1141 aatatattag gaggggttaa aacctgttta aaagatgaat atatgactat gacatatgta
     1201 agtcaggggt ttttagagct tacaggttac tcttgtgaag atttaaataa aatatttaaa
     1261 aatagattta aagacatgat ttataaagat gatttagaca agtttaattg tattattcta
     1321 aatcaattag aaaaacaaga taaaattgaa gttgaatata gaattgtaag aaaagatgga
     1381 agtataattt ggattttgga gagaagtaag tctataaatg ataataaagg aagtgagtgt
     1441 attcagggag ttttaactga tataacagaa cttaagaaaa tacaagaaga attagattca
     1501 aaaagatttg aaatacaggc tatcaatgat agtattgttg gtggaatagt gataactgag
     1561 ataaatgatg attttgatag tatatattta aatgaaggtt atttaaatat gattggatat
     1621 acacgcaaac aactggaaga tgagctaaat aataaattaa tcaaacttat ttttaaagat
     1681 gatagaacag aattaattaa tagtataaac aaacaactca aattaagtga tacatttgca
     1741 tctgaacaaa gagttgaaaa aagggatggc actattatat ggattttatt aaaaggaaga
     1801 aagatgattt atgataatgg aaaatctgta ctttgttgtg ttattgtaga tattacttct
     1861 gctaaattat tagagcaaga acttaaaaag agtgaagaac gtcatgaaat cattctaaag
     1921 cagacgcaaa atattataat agaatgggat atagaaaaag ataaaatgaa tatatcacct
     1981 aattggtata ataagtttaa atatgaccct ataactgaaa acgcaacaaa aaatataatt
     2041 aaatcagtag atatatatca tgatgatatg gataagttta tttcattaat taaacttact
     2101 tcacaaggta gtgagtatga agagtgtgaa attagactta gaactagaga tactatttac
     2161 gcttggttta gtataaaggc aacaacttta tttgataata atggtaaacc ttataaagta
     2221 gtaggaagtt tgactgatat tgatgaatca aaacgagaag ctgaaaagtt gaaagaaagg
     2281 gcagaaaaag accctcttac agatctgtat aataaaaaag catcacaatc acttatagag
     2341 gagtacataa cattggataa aaaaagaaaa ggtgctttaa tgattattga tattgatgac
     2401 tttaaaggaa taaatgataa tttaggacat ttatatgggg atgctgtatt aagtgaaatt
     2461 gcaaatgacc taataaatct atttagagca accgatatag taggcagaat tggtggagat
     2521 gagtttattg tatttataaa agatatttct gaaacaagag atattataaa taaagcagaa
     2581 gaattggtaa aaacatttga aaggtctttt ttaggtaaag gacgaaatta taaaatatca
     2641 ttaagtgtag gaattgctaa gtttccacaa gatggtatgg actatatgac actatttaga
     2701 aatgcggata tagctcttta ctcagccaaa gggaagggca agaattgtta tacattgtat
     2761 gataaaaaat ttgataaagt ccaatatata actaatacaa gaaatgcagg agatagtgat
     2821 aatgtagaga tgtcttttaa agaacatatc acaaattata tatttgatat actatatgaa
     2881 tcaagtgata tagaagtggc agtaaattta attctaagta ttgtaggtaa acactttaat
     2941 gtgagtcgca tgtatatatt tgaaaattca gatgataatc gagtatgtaa aaatacattt
     3001 gaatggtgta acgaaggtgt aacctcggaa attaataatc ttaaaaatat ttgttacaag
     3061 gatttaggca actatctttt aaattttaat gcagatgggt tattttattg taatgatatt
     3121 gaaaatttag gtagtgatgt gaaaaaagta cttaagccac aaggcatacg ttcaatatta
     3181 caatgtaaaa ttttagaggg taatgaattt agagggttca taggatttga tgaatgttca
     3241 tataatcgta tgtggactaa tgatgaagtg aacactttga catttatatc taaaacgctc
     3301 agtatgtttt tattaaaaat gcgtattcag agtaagctag aagagtcttt aacaattcaa
     3361 aaatctgtga tggataatat ggatatatgg gcatatgttg ttgacaggaa tacctatgaa
     3421 ttactattta tcaataaaaa aatgcttgag tcagcaccaa acactcatgt aggtgattta
     3481 tgttataaag cgatatggaa taatcaagat aaaccttgta aaaaatgccc tatgatatat
     3541 ttaaaagatg gtgatgatac ttataaatca ttcatgcata ataaaaagtt tgaggtacag
     3601 gcaaatataa ctacaactac tttgaaatgg tttgatggta aagatgcatg tttaatattt
     3661 ggaagtgaaa taactagtat tgaagctgaa gaaaatactt aaaatactgt gttatatata
     3721 tacttaaaat cgcaatttga atttattaga cttaaaatgt ctatataaat catttaagtt
     3781 gtggttttaa gtatttttta tgatataatt aagcgttaag aacaaaatta aaagatactt
     3841 ttgtataaaa aaatatttgt taaatatata ttgagcaggt gataatatgg aaaataagtt
     3901 tttaccaata agcaaacaag atatgataga tagaggatgg gaagaattag attttgttct
     3961 agttacagga gatgcttatg tggaccatca tagttttgga acagcaataa tatctagagt
     4021 tttagaaagt gctgggtata aagttggaat aatagctcaa ccagactgga gaactacaga
     4081 tgattttatg aagctaggaa aacctagact tgcatttcta gttaatgcag gaaatatgga
     4141 ttcaatggtc aatcattatt ctgtaagtaa aaagcataga gaaaaggata tgtattctcc
     4201 aggtgggaaa atgggttata gaccagatag agctactata gtatattgca ataaaataag
     4261 agaagcatac agtgatgttg caatagtaat aggtggaatt gaagcgagct taagaagatt
     4321 tgcacactat gattattgga gtgataaggt tagaaagtct atgttaatag atagtggagc
     4381 agatttatta gtttatggta tgagtgaaaa gcagattgta gaagtagcca atgcacttaa
     4441 tgatggatat gacccaaaat acattagaca tattgatggg acatgctata tttcagatac
     4501 attggaagaa atatatgata aatatatatt aattccatca tataaagaaa tttgtgaaga
     4561 taaaatgaag tatgtagaag cttttaaaat tcaatatgat gagcaagacc catttagagg
     4621 taatataatt gttcaacctc atggaagtaa gtatctggtt caaaataagc cagaaaaacc
     4681 acttagtaga gaagaattag atgaagtata tggattacca tatcagaaga cataccatcc
     4741 tgtatatgaa aaatttggag gaataccagc tatagaagaa gtaaaattta gtatagtaag
     4801 ttcaagaggc tgttttggta gttgttcatt ttgtgcaata acatttcatc aaggtagagc
     4861 agtgcaaagt agaagtgaaa aatcaattat tgatgaagca ataggaataa caaatttaga
     4921 tgattttaaa gggtatatac atgatgttgg aggtccaaca gctaatttta gaagaccagc
     4981 atgtaaaaaa caaattacta aaggtgcttg taaaaataga caatgcttat ctccatcacc
     5041 atgtaaaaat ttagatgcag accacagtga atacttacat ttacttagag ctgtaagaaa
     5101 attacctaaa ataaaaaagg tgtttgtgcg ttctggaatt agatacgatt atgtaatggc
     5161 agataagaac aataaatttc ttagagagtt gatagagcat catgtaagtg gtcagttaaa
     5221 ggtagcccca gaacatatat cagaagaagt tttaaagtat atgcaaaaac cagcaggaaa
     5281 aacttatgat aaatttagac agaagttttt tgctataaat gaagaattag gtaaaaagca
     5341 atatttgata ccctatttaa tgtctagtca ccctggttct accttaaata gcgctataga
     5401 gttagcagag tatttaagag atactcacta ccaaccagag caggttcagg atttttatcc
     5461 aactccaggg actctatcta caaccatgtt ttatacaggt attgacccgc ttacaatgaa
     5521 gccagtttat gttcctaaat caaaacgtga caaggcaatg caaagggctt tattacaata
     5581 tagagcacct agaaattatg atttggtata ctctgcgtta gttgaagcag gaagagaaga
     5641 cttaattggt tttggacata gatgtcttat aaagccaaaa aatgaaaagc cttattttaa
     5701 tagaaataat tctaaaaaaa atgtatctaa aggaacaaat aaaaacaaaa aaactaatac
     5761 aaataacaga agtaataaaa atcaacaaaa aagttcaact aaaaaaaaga aaaaacatta
     5821 aagtataaat aaaaatactt aaaaaagaag gattattcga taaattaaca gaaattagta
     5881 taaataaata taattatttt aatgacgtta cctttgggag gaagagatga aaaagtatat
     5941 ttctataata ttattagtgg tgttgactat ggttgttttg gtaggatgtt caccaggaaa
     6001 agataaccct aaaaataagg agctatcagt tgtcaaacaa gtaaaagtag ctgttccaga
     6061 tggactacca gcagtggcaa tagctaaact tgcaaatgaa aatccagaga ttaaagaagg
     6121 atatgaaaca gtgtatagca ttgaaaagac acctgaagcg atttccacta gagtgatgaa
     6181 aaaagatgca gatatagcta tagttccttc aaacatggct gcaatagcgt ataataaaac
     6241 ttcatcatat aatattgttg gaactgtagg aatgggttca ctttatttgg tgtcaacaga
     6301 aaatataaaa gattactctg atttagttgg taaagaagtt ggatgtactg gaaaaggact
     6361 tactccagac ataactataa aaagtctttt gttacaaaag gatataaatt attcagatat
     6421 aaaatttaat tatgtaaatt cagcaagtga actggtacca ttattggcta ctggtaaagt
     6481 taaaacaggt attgttccag aacctgcact tagtgctttg atgtctaaaa atccagacat
     6541 aaaaattata aaaagtttaa atgactcatg gaaggaagta agtggttcaa aagatggtta
     6601 tcctcaatct actttaattg taaagtctga atttttaaga gataataaag attttgttga
     6661 ttcttttgta ggtcaattat caaatagtat agattgggca aacaaaaatc ctgaagaatt
     6721 aggtgcatat tctgaaaaaa taaaaatatc tactgaatca aagataatag gaaaatctct
     6781 tgaaagagct aatttgaaat atattccagt taaggacatg attaaagatt ataaaaatta
     6841 ctatgagaaa ttagctaatt ttgatgataa gacattagga ggcaaagtac cagatgaagc
     6901 aatatacttt gttgagaaat aaaaaaaata aagaacaatt tatagataaa atggaggtaa
     6961 cactttcttg tgttatcctc ttatttttat ggcaaattat tgctttaaaa attgataatg
     7021 atattttttt accaacagta ggtcaagtgt ttaatagtat aaaagagatt atgctaaatt
     7081 ctagtttcta tatagatata ctttactcta tggggagatg tttttttagt tttttactgg
     7141 ctatgatttt tgctattata ctttcaatag tgtcttattt aaatagattt tttagaaact
     7201 tattaaagcc aatcaatgct ttgacacgtt caataccaac tatgatattg gtagtacttg
     7261 cacttatttg gtttgaaaag gatagtaccc cttttatagt tggatttact atagtttttc
     7321 ctatattgta tgataacgtt ttgggcgcta tattgaatat agacaagaac ttattagaaa
     7381 tggctaatgt gtataaaata aggtttatag ataaggtgtt aaaaatttat ttaccagcca
     7441 taaaatttca aattatatct atactagtat caacattttc tttaagtttg aaagttgcta
     7501 tagcaggaga ggtgtatagt caacctacat atggtattgg aactatgata cagactgaaa
     7561 aaattaactt taacacatct ggaatatttg catggataat tattgttctt ttaatatctg
     7621 ctattttaca aatagctcaa aagtttttag caaggagagc ttttttatgg aaaagataaa
     7681 gttgagtatt gaaaatataa ataaaagata tgatagtcgt attatattta gagattttaa
     7741 tattgatttt tatgtaaatg aggtaaattg tatccttgga aaatctggtt gtggaaaaac
     7801 tacactcttg aacataataa gtgggattat taaaaatgac accaacaatc taaatatcaa
     7861 agaaaattta aatagagtag gaaataagct tgaagctagt tacatatttc aagatgatag
     7921 acttatagac tggttaacag tagaagaaaa tataaagata gttgtaaata aatactataa
     7981 taaaactcaa ttaaataaaa tttgtgatga atacttggaa ttggttggaa ttagtgatta
     8041 taaaaaattc tatcctcaaa tgcttagtgg aggtattaga caaagagtaa atattgctag
     8101 agcttttata tatccatcaa aaaacattat aatggatgaa ccttttaaat ctattgatgc
     8161 aaagaataca cagcttataa tggataattt tagaaatata ttaagaaaag aaaagagaac
     8221 tgtgttattt gtaactcata atattgagga agcattgttt ttagcagata ggatatttat
     8281 tttaggagac tctccaatta gaataaaaaa aatattaaaa aattctaagg aattggaaaa
     8341 aaatgaagtt ttgaagttga tatagagtta taataatact gtcgttttaa aatatatttg
     8401 gttaaaatct ataagactat attaaaatag tacagaatat tcaaaactgt gtataatgtt
     8461 cgtattttat aaaaaggaag tgaatgtatg gttaagacta atgctatgag aatattagat
     8521 tcaaataaga ttgattataa agtaatgtct tatgaagtga aaagtgaaca tgtggatggt
     8581 gttgaggtgg cacatgatat aggaagagat gtaaatgaag tgtataagac attagtaact
     8641 caaggagtta gtaagaatat atatgtatat gtaataccag tacatgaaaa cttagactta
     8701 aaaaaggctg caaaagtagc taaagagaaa agtgtagaga tgattcatgt aaaagatata
     8761 aataagctta caggttatat tagaggcgga tgttctccta taggtatgaa aaagttttat
     8821 aaaacttttg taaatgaaag tgcaaagaat ttagatacaa ttattgtgag tgcaggaaaa
     8881 ataggatatc aaatagaact atctccattt gatttacaaa gacttataaa agtagaattt
     8941 gtagatgtaa taaaaaaata agaattacat ataaaatata gagcttatat tctcaataat
     9001 aggtgttgta atattaataa aatgaataag aattttaaaa taaatattca gttacttatg
     9061 tgtaattggt ataaaaatat tgagaaattt taaaaacaat ggtaaagtgt aagtataatc
     9121 acgaatattt tttataaagt aatggaggga tgttcgcttg agaaaaataa aatctgaaca
     9181 aatcgtcgag caggtaaaaa aactttgtat agaagcgagc ctatatttag gagaagatgt
     9241 tttaagttgt ataaaagaaa aagcgaaaag cgaaaagagt gaagttggaa aaaatatatt
     9301 gaatatatta gttgaaaatg ctgaaatagc taaagaaaaa aatataccaa tatgccaaga
     9361 tactggtatg gctgtatttt ttgttgaaat aggacaagaa gttttaattg aaggagacac
     9421 acttactgat gctataaatg agggagtaag acaagggtat gaagaaggct acttaagaaa
     9481 atctgttgta agtccaatta acagagtaaa tacaaaagat aatacacctg ctgttattca
     9541 ttatgatatg gtaaaaggtg ataaaattaa aatagaattt gcagctaagg gatttggaag
     9601 cgaaaatatg agtaagatga aaatgttaaa gccatcagat ggattggaag gaattaaaaa
     9661 attcataata gatacagttt cagaagctgg acctaaccca tgccctccta tggtaattgg
     9721 agttggtata ggtgggactg tggacaaatg tgctcaaata gctaaaaagg cactttttag
     9781 agaactaggt gaatttaata aggacgaaaa tatagcaaaa cttgaaagtg aactattaac
     9841 agctattaat aaacttggta taggacctca aggcttaggt ggtactacaa cagctttggg
     9901 attaaacata gagacttttc ctacacacat agcaggtcta cctgttgttg taaatataaa
     9961 ctgccatgca tcaagacata agaatgtagt aatttaggag gaaaataaaa tgataaaaat
    10021 aactacacct gtaaatgaaa ttgatatagc aaaattaaat tgtggagata caattagttt
    10081 gagtggtata ctttatacag caagagatgc tgctcataaa agattgatag attgtataaa
    10141 taaaggtgaa gaattaccat ttgacgtata tggtcaagga atatattatg taggacctac
    10201 tcctacaaag ccaggagaag ttataggtgc tgcaggtcca acgacgagtt atagaatgga
    10261 tgatttgact atacctcttt tagaaagagg tcttagattg atgataggca agggaaaaag
    10321 aagtgatgaa gttatagaag gtatgcaaaa atacggagca gtttatcttg ctgcaatagg
    10381 aggagctgga gcgtatattt caaattcaat aaaatcatgt gaaattattg catatgaaga
    10441 tttaggagct gaagctataa gaaagattgt agtagaagac cttaagctta cagtagcaat
    10501 agatagctat ggaaataata tatatgagca aggtcgagca atttatgaat gtaaataatg
    10561 tttagaaata aatatgaata tcaaaattaa aaatagttgt gtatatttta aggaggaaaa
    10621 ttgatgtcta aggattatgc aaaattggca ttagaaatgc atgaaatcaa caaagggaaa
    10681 gtaagtgtag aaagcaaggt agaaattaaa actaaggatg atttatcaac agcatataca
    10741 ccaggggttg ctgaaccttg tttaaaaata catgaaaatc aagatgatgt ttatagatac
    10801 acttctaaag ggaatctagt agcagtagtt agtgatggaa gtgctgtact tggattaggt
    10861 aacattggtg ctgaagcatc tatacctgtt atggaaggta aagctatatt atttaaacaa
    10921 tttgcagatg tagacgcttt tccaatatgt ttaaagacta atgatgtaga tgagatagtt
    10981 aaatctgtag agttgatgga gcctgtattt ggaggtataa atttggaaga catatcttct
    11041 ccaagatgtt ttgagataga agagagatta aaaaaatctt taagtatacc tgtttttcat
    11101 gatgaccaac atgggacagc aataatagtt gcagccgcca taataaactc aataaagtta
    11161 attgaaaata aaaaaataga agatttagaa atagttataa acggagcagg agctgctggt
    11221 atagccatag caaagatact acttaatatg aatgttaaaa atataatctt atgtgataga
    11281 agtggagcat tggaagctag tatagaaaat ctaaactatg ttcaaaaaga aatgttaaaa
    11341 gtaacaaata taagaaatga aaaaggacct ttaaaagata ttataaaagg aaaagatgta
    11401 tttattggtg tttcaggtcc aggagctgtt acaaaggaaa tggttgaaag tatgtctgag
    11461 aagccaataa tattggcaat ggcaaatcct actccagaga taatgccaga agaagcaaaa
    11521 ttaggtggag catttataat gggaacaggt cgttcagact tccctaatca agtcaataat
    11581 gtactggctt ttcctggaat atttagaggt gcacttgatg ttaaggctaa agagataaat
    11641 gaagaaatga agatagctgc tgcatatgca attgctaata caataagtga tgaagaaata
    11701 tgcccagagt atatattacc agatgtattt aataagaatg tagtaaaaaa tgttgctaag
    11761 gcagttaaag aagcagcgat aaaaactaaa gtaaatagaa tataaatttt tatatgttca
    11821 tatatcctta tattatcggg tatatgggca tttttattat aggtttatac tcatatatcc
    11881 ttacttttat atacatatct ttatgattaa aggtgttaga aaatgtagta aattttatac
    11941 tatcagactc tatctttatt actttagtag atacatggtt gtaaaggttt atagaaaaaa
    12001 atataggaag gcagattttt attgttgcgt atatatgaga atgatataat atgaatatta
    12061 taaattaata aatttaaatt tatggaggta caagagatga tggaatataa agttaatgac
    12121 caagaactta atgcttcaat gtttatccca ttcgtcaaca ggatatggcc aggagattat
    12181 gatatggata aaacacagac tgcattatca aaaactttga atataactgc ttatgatgat
    12241 aaagtgcttg taggatgtct gcgagttctt tcagatggtt attattttgg aacaattaca
    12301 gaattgcttg ttcttccaga gtatcagaaa caaggcattg gtagtaagct tttccagctt
    12361 gctaaagaga acaccccaac tatgctatat tttggtgcac agccaggagt agaagcgttc
    12421 tatgaaaaaa atgggtgcca aagaagtctg caatcctaca taataaaaaa gaaagatagt
    12481 tattaaagtt ccagctttca ataatcttaa aagtacaata ttttagtgga atcatttcat
    12541 tttttattac taatatgaca taaacatgtc atattagtat tgatatactt ttaatatggt
    12601 taagagccaa agaaaaatat aaggagtgat ttaaaatgga agaaactaaa ttttgccaat
    12661 gctgtgcaat gccaatggga caaactgatg aattatatgg gacaaataaa gatggaagta
    12721 aaagtactga ttattgtagt tattgtttta aagatggtga gtttacagct gatataagta
    12781 tggaagagat gatagaagtt tgtatccctc atatgcttca aagtaataag gatatgactg
    12841 aagatgaagc aagaacaatg atgaacaatt tctttccaac attaaaacgc tggaaataat
    12901 tttatgcaaa attagttata tgcaaaacat gaaatatggt gttgagaata aggggtggtg
    12961 tgctttatag cataccacct gttttgttac ttggagttca gttattttcc taaatatttt
    13021 aaatatacta ttttagataa gttccctctt catcttgata aattaatcct tttttcataa
    13081 gcactccaag agctcgttta aaatttttct tacttgaatt aaataatata gaaatatctt
    13141 tagggtcaga tttatcatta aatctcatat atccatcaga accttctaaa taacttaaaa
    13201 tcttattttc taaagtatca agttcatcct ttctatttcc tctaggagaa agaccaagtt
    13261 ttccatcttc atatattttt atgacatgta aattttcaag aacatcacca gtttttagtt
    13321 cagtaaagta ctcattatga agtattacac cagcatattt attgtctaca catatcattg
    13381 cagaattatt agtttggaat ccatatacaa ctccagttac aatatctcct acattgtaag
    13441 tatggtctgt taataagtat gaatctatgt ctgtagtagc agcaattctt ttgcttttgt
    13501 ctaaatatat ataaaataaa tatttttcgt ctctaaatag tggataagtc ttagctttaa
    13561 aaggaactaa tatgtcttta ggaagtccca tatctataaa agtaccaatg tctgtattat
    13621 caactacttt taaatgtgct acatccccaa ccttagctaa aggcggtatt aaagttgcag
    13681 cagttctgtc atcagaatct ttaaatatga aagcatttac ttcatcacct atgtttattt
    13741 cattttttcc gattaatcta ttatgaagaa gtatatcgtc ttttgtgtta ttagtttgtc
    13801 catccaaaaa atatccaaat tcagtttttc tttttactgt aagtttatta aaatcaccta
    13861 tgtttatcaa attaaaatca tcctctcatt ttatatttat aatttagcca taatatactt
    13921 atgtagtagt ttgtcacagt atccatatta gtattagtga aatttacaat aaataatata
    13981 gttatttatg tatttttcaa ttctctagct tcttatatag tataaactcc aatttctttt
    14041 ttgtctttag gttttattaa aatttttaca aaataagtta aaaagtattg aaataaaatt
    14101 tcttttaaag tataattggt atataccaaa attggtatat accatatcga aaagtggtga
    14161 ctatatgaaa atagtaaata aatctagtaa tataccattg catactcaat taagttcaat
    14221 aataagagag atgatagaaa ctggtgagct taaagagggc gatgcaataa tgccagaaag
    14281 agaactttgc aacatacaaa atgtaagtcg tatgacagta aataaaacca ttgttggatt
    14341 agttacagaa ggacttcttt atagggtaca aggtaaaggg acattcgttg caaagcaaaa
    14401 aaagaagtat cagttttcaa atgtaaaagg ttttactgat gttatgaaag aaaagggagt
    14461 aaacataaaa acggatatat tatcctttga gatggaacta ccagatgatt tagttaaaag
    14521 aaagcttgga ataagtgata atactacaaa tatatataag attgtcagac ttagatatac
    14581 tgatggagag ccatttggat tagaaatagt atatctatca gaagaaatgt gtaagggtct
    14641 aacaaaaggg atattggata attcttcatt atatagggtt ttaaatgaaa aatatggcta
    14701 taagatacaa aaagctgaac aggtaatgga gccagtaata ctttcagatg aagaaagtaa
    14761 gcttcttgaa acagatgaag gtgctttagc tcttaaactt catagaaatt cctataatag
    14821 agaaggtagc cctatagaat ataccatatc aatatttaga acagataaat atcaatatga
    14881 aatagtattg agtgagtagg tgaataatat gaaatgttta attaatggaa agataatatt
    14941 aaaaaatcaa atcttagaaa ataaagtatt agtatttgat gaaaaaataa ttgatatagc
    15001 tgatagtgta ccaaaagatt gtgaagttat agatgctgat ggaaaatata tatctccagg
    15061 gcttattgat attcatatac atggaaacat gggaaaagat acaatggatt cgactgatga
    15121 atctattgaa acaatttcaa agtcaattat gagacatgga gtaacatctt ttcttccaac
    15181 tacaatgact atggataaag aacatgttta tgatgcactt gaagttatta aaaaagccca
    15241 aaatagaaag cttgaagggg cacaagtatt aggagcacat ttagaaggac cttttataaa
    15301 tgagaattat aagggagcac aaaatgaaaa atttataata aattcaaaat acgaatttat
    15361 aaaagaatat aaagatgtta taaaagttat aacttatgct ccagaaaaag atattgattt
    15421 tgattttact agagaaataa aaagatgtac agatatagtt ttatctattg gtcatagtaa
    15481 tgcaaattat gaccaagcta aagaagctat aaatttagga gtaactaatg taactcacat
    15541 gttcaatgca atgactggtt taaaccatag agacccaggc gttgttggtg cagcacttac
    15601 aactaatgtg tacagtgagt taatagcaga tacaatccat ataaataaag atttatttca
    15661 atttatacta aataataaag gaaaagaaag actgatttta ataacagatt ctatagaagc
    15721 tggtggtctt gaagatggca attattctct tggaggtcag aaagtcatag ttaagggtaa
    15781 tgaagctaga cttgaaaatg gagcattagc tggaagtgtg ttgtctttaa ataaaatggt
    15841 atttaacttc ttagataata caaaccttaa agtgaatgaa gcaattaatt tagcttcttt
    15901 aaatccagca acatctttgg gaataaatga caaaaaagga agcttagaaa taggtaagga
    15961 tgcagatata gctgtatttg atgagaattt agattgtaaa atgacacttt gcttggggga
    16021 cgtagtatat aaaaatatat agtatacaaa acaaacctta aagacagtaa aaacaatata
    16081 aaaaataata aattaaactt acattagtaa aagtagtagt taaaatttag gaggaataag
    16141 atgagagtat tagtgtgtaa agattatgat ggaatgagta aaaaagccgc tgaaatgata
    16201 gcagctcaaa tagttttaaa accaaattca atactgggac tagctacagg aagtacacca
    16261 gtaggtatgt atagagattt agtaaaaaaa tacaatgata atatagtaga tttttcagat
    16321 gtgatgagct ttaatttaga tgaatattat aagttaccaa tatctaatga ccaaagctat
    16381 gattatttta tgaaagaaaa tctatttaat catgtaaaca taaagccaga aaatactcat
    16441 ctaccaaatg gaatggctga tgatattgaa aaagagtgta tgaactatga agcttctata
    16501 gatgcagctg gtggtataga tgtacaagta cttggaatag gtcgtaatgc tcatataggt
    16561 tttaatgaac ctgatactaa atttgcaaag agaactcatg tagttgaact tacagagtct
    16621 acaatagaag ctaatgcaag attctttaaa tcaagagaag atgttcctaa aaaagctgta
    16681 agtatgggaa taggttcaat attgaagtct aagaaaatat tgcttttagc ttcaggagaa
    16741 gaaaaagctg atgcagtata taatactgtt tatggagata taactccaga agttcctggt
    16801 tctatacttc aacttcataa agacactata gttatagtag atgaagcagc agcaagtaaa
    16861 ttgaatccaa aagattataa attagtataa gaatagaatg tagtttaagt aaaaaatagc
    16921 tatcattgag taatcaaatt tatttaatga tagctatttt tatttaaatt ttttacttct
    16981 ttttatttaa attagttatt attgtttaaa ttacttgctt attacttttt acgaaaactc
    17041 gttatcaaga agtatccaaa taagatgtta aaaattatta tgtttagtgg gattctatca
    17101 agcataataa aattacctat aattgggata ttaccaactg gaaacataaa gaagaataca
    17161 ggcatcagtg atagtaaaaa agatggaaaa caaagaagaa gtaatcttgg aatatcaata
    17221 taccaactac catccttttt gtattctcta atcaataatt cagttcctag taataaacca
    17281 ataccaccag aggaaattaa attaataaaa atgtatcttt taaagtcttg catataacca
    17341 gtgtaaaatt gagtatagaa atcataattt aatttaacag ctaaaaaata cataagcaat
    17401 agaatgatta aaaataaaag atatctaaga gcttttttca ttttaaattc tcccttcaga
    17461 aatactttat tcagttttat tttgttggaa tctgatttta tcatttagtt taagaaatcc
    17521 ataatattag tgaagaatga aataagtatt atatatgaat aaatattttt agaattaagg
    17581 tgaattgtag tgcttaatat aactataata ttaattgttt gaatattcaa taaaaagttt
    17641 gaaaaatatc aattttgata taaactgaat aaatacagtt gaatattaaa ttattatttt
    17701 atcaaaaata tgcaaagtac tacaccttca aatatcacct aaagtaatta aaaggcaatt
    17761 aaggtgaagt agttcaagta tttctaaaat aagtttaata aattctataa taatttattt
    17821 tttgccttaa aattgcctta aaatccaaac aattttaaaa ctaatgtact ataaaaagct
    17881 ggtataattt aaatatagta aaaaataaga tttatcttat ctattgggat ttaattattt
    17941 aatcaatata tttataccat ttaatttaaa taaaaagtct attaaggagt gagcttatga
    18001 gtaagttaat ttatatagca gatgatgaag ataatataag aaatttggta aagacattct
    18061 taaaaaatga aggtcatgat gttatggatt ttaaaacggg tgatgaactt ttagaacaat
    18121 ttaatataaa ggagtgtgac ttagtaatac tagatataat gatgcctggc tcaagtggtt
    18181 ttgaagtatg tacaaaactt cgtgagaaaa gtacagtccc aataatcatg cttactgcaa
    18241 gagatacaga tatagattat attacaggga taaccttagg tagtgatgat tattttacaa
    18301 aaccatttag cccaatgtct ttggttatga gagtaaaatc tatatttaga aggatagagt
    18361 ttgaaaagaa acaaaattat gataaatata gtaactcgat agatatggaa ttaaaatttg
    18421 gtgatgtaat aataaataaa aagaataaaa tcgttacaag taaaaatgta aatatagacc
    18481 ttacaccaaa tgaatataac ctattgactt atttgtttga aaatatagat agagcagttt
    18541 ccagagatga attattaaac aaaatatggg gatacgatat agaagttgag acaagggctg
    18601 ctgatgatac agtgaagaga ttacgtaaaa aaattttaaa tacaaatatt ttaatagaaa
    18661 ctgtatgggg gtttggattt aggttgaagg agaaaagtta agatgaagaa tggaaatgac
    18721 aagaatagaa ttggaaagat taaatccaaa tttaaattaa agttctcaaa gaaaaatata
    18781 agcataaaaa ctaggatttt gttgagtata ctttgtttga taattatagt atttatgata
    18841 atattagtat cttttaatgt atttcttgat acatatataa aaacaactgc gaatgaagaa
    18901 ttgacaaagt ctacagaaat agtagaacat atggatagta attttagacc tattaagcca
    18961 ccacaagatg atggaaagct tcctcccaaa gtattatcta attttatgag aaatgtacaa
    19021 gataaagtta ggatggcaga aacacaatca gatgctgatg ctatggtagt tgattctaag
    19081 tataacctga tatttccaac aagagaggat gatttcttaa aaaatgttga tgagatggag
    19141 ctgattaggg agtgtattca aaatgaaaaa ttagatttaa attcagatga aaatacacgg
    19201 atatctacca gaaataagga ttattatatt tcaactgtaa aaattactag tatatatggt
    19261 gaacaagaca agtatcttat actttttata gacatatcaa aaactctaaa tctagcacaa
    19321 aagataaata ttgtattaat atctgtaatg tgttttgctg gaatattagc aatatttaca
    19381 gctgtgattt tatctgagaa aattgctaaa ccgataaaag agttgtgtga gtttgtaaaa
    19441 aaaatgggtc aaggagattt caaaagaacc tactttgact tttcagataa agaattagta
    19501 gagctctcaa ctgttatgaa taagagtgct gagtatctgg ataagtatga taatgaacaa
    19561 aaaatatttt ttcaaaatgc atctcatgaa ttaaggacac cacttatgtc tattaaaggt
    19621 tatgcagaag ctataaaata taatgttata gatagtaaac atgcaagtga tataattctt
    19681 gaagaaagtg ataggttaag tgatatggtt gaagatttat tatacatttc taaaatagat
    19741 aatataacta aagattttga acttgtagaa tgtgatttaa gagaagtact ttcaaattgt
    19801 ggagctaggc aaaatgtaag agctataaat aaggggataa aatttattta tgaatttgat
    19861 gaagaaatgg ttttatttga atgtgatgaa aaaaacattt ccaaagcatt tatgaatctt
    19921 atagaaaatg ctttgagata cgctaagagt gaaataaaaa tagtgtgtaa gtataatcaa
    19981 aaaaatatag ttgtgataat agaagatgat ggaattggaa tcaagaaaga agatttacct
    20041 catgtttttg aaagattcta taagggagta gggggaaatc atggaattgg attatcaata
    20101 gttaaatcta ttgtaaataa gcatggtggg cgtatatacg tagaaaatgg caaaaaagga
    20161 gctaaattta ctatattatt taaattgtaa aaaaattaga gaaaatatag agatgtttga
    20221 agttgttttt tatatatatc tgttgtatac tgttgaaaaa agaacaatat taagctgaca
    20281 tatattacga tagttgacat ttaaagtaaa tgcttaaata tagatacgaa aggaaaagta
    20341 aaatctgaag tgtatttagg tttaagtaag cttcttattt tataaaaaat atgtatgaaa
    20401 gaacagctga tttttaatat tacaaattta gtaacggttg tatttgaagc ttttgtcatc
    20461 catatgtttt tatcagactt tttaggtcag aaagaagggt atataaaagt tgtaagatat
    20521 gctaaactgg gttttataat atgtttagga ttctgtaatt taataacttt aaaccctaaa
    20581 atcacaatgc ctttaatttt catccttatt ttttcaacat catttttata taaaggaaat
    20641 ttaaagacac gattgtttac tactgtttta ttaagtatat tctttatact gtcagaaatt
    20701 gtagttacaa gtatttttgt tttatttgta aaagaaggct ttgaaataat gcttgaaaat
    20761 aatagtataa gagtattggc gacaatttta tctaaaattg tctttttgtt gacttgtaaa
    20821 attatttgtc tatttaaaaa ggatgtacat ttagatatgc ccataaagta ttggctccct
    20881 ttatttttaa tacctatatt tagtttattt ctgtctgttt ctatctttga tgtaagtaaa
    20941 ttttttagtc ttgaaagttt aaaatttcta agcttaatat ctagtgttgg tattttatat
    21001 attaatttta ttgtctttta tttatttaaa tttattatag ataaaactaa actatctatg
    21061 aaatatgaac tacttgaaaa agaaataatt cataaggaag aattaagact tagtaatgag
    21121 tgttataaaa ttattgttga acagactgat tctgtcgttt tcgaatggaa tatcaaagaa
    21181 aataaatcat ttgtatcgca ggcttggact gaaaaatttg gatataacaa tgcatgtaag
    21241 aatattttta aagaaattaa agataaagac ttagttcatt ctgaagataa agctatattt
    21301 gaaggttttc ttgaaagtat taaaaaaaag aatatgcaca atcaagcagt atatcgttta
    21361 aaaaaatcta atggtgaata tatttggtgt agaacatcaa ttacaagcat ttataatgat
    21421 gaaaatgaac ttttaagagt tgtcggagta attgtagatg tagatagtga tataaaaaaa
    21481 tatgaagagt taagaactag agcagaaagt gactcattga ccaatatata caataaaggt
    21541 acatttgaaa aattagtgga agaaactata gttatgaata caggtgataa aaaagatgca
    21601 ttattcatta ttgatttgga tgattttaaa gaaataaatg ataattttgg tcatccattt
    21661 ggtgattttg tactaaaaac atttgctgat aagatacaaa ctagttttgg tagtaaagac
    21721 ttagtcggaa gaattggtgg agatgaattt gttgtatata tgcaagatta tgttacagaa
    21781 gtaaatttgc ataaaaaagc taaagaatta aatagagtac tttcggataa ttacactgat
    21841 ttgagtttta gttttgatgc ttctgtcagt ataggtattg caaggtatcc tcaagatgga
    21901 acttcatttt ttgaattgtt taaaaatgca gatagagcac tttatagcat aaaagcatca
    21961 ggtaaaaatt catattgttt atttgaagaa gaactatatg tacagtgaat ttgaatttag
    22021 attttatgtg agagtaagat ttatatatac ttgtggctgt ctgatttata aaatatgtta
    22081 ctttgtatat ttttatatgt ttcagtaagg ttaattaaca tgagcaaagg tgtaagtaaa
    22141 tttatattag tttaagttta cttacacctt tataaaaata caaataatag attcatttac
    22201 attcttaaat gattaaagct ctttaccttc taaacatgca taacatatcg ttataattcc
    22261 agtaaataag actaaaatgc ccattactaa gattacaatt tcatcatcta ttactccaaa
    22321 tgtttttaaa gtaacttatt tcgctttgta tcataataat ttgtctcttt ttacatttta
    22381 acttctgctg agatttgagt tagatattca ttagaatctc gcacacttat atcatataga
    22441 agatattctt cataaacaaa atccccaata cgtaaattat tctgattaaa aaaatttaat
    22501 aacttttcat aagccttgta aatagtttca taactccctt tatgataagc agttacataa
    22561 agtccctttg gtttgattga tacaggatat ttcttgtact ctttattaac ttttgtaaat
    22621 aaatatgaat atgattcaaa gtctttatta attatatctt cacctttaat gattgagcca
    22681 atcgtaccat aatcactcaa ttcatagttg tccataaagt taatacattc atataaaaaa
    22741 ttttcttcct catctcctaa aaactgttct ttatatgtaa caggtgtttt gaccaagtac
    22801 tcttcttcat gttcttttaa agtaatagta ttcgcatcta tattacatgc acttttagta
    22861 aatgaaatag tagattccat tagagcttga atattattta gtttttcaat ttcattttta
    22921 atatcaatgg ttttcttttc taaaagctca attagtaaat taggtgttct tttgtctata
    22981 taagctttaa tttcttttaa aggcattttt acttccctaa gtgaagaaat tataccaaaa
    23041 atgctaagtt gatggtatga gtagtatcta tatccatttt ctttctttaa ttctggtgag
    23101 aaaagaccta tatcatcata atggaaaagt gtttttttat tgattccaca aatctttgca
    23161 aattctccag ttgtaaaata attttttgaa acttgactca taattataat tcctccttga
    23221 ctatcctgtt acagtatagt ttatcatagt attttgtaaa ggagtgtgat tatatgaaaa
    23281 aattaattca ttttttaaaa ccatatagag ttttaattgt tgtagttttg atatttacgt
    23341 ttttacaaac acttggaact ttgtatatac caacacttac agcaaatata gtaaataatg
    23401 gtgtagtaaa gggagatata gattatattg taaaaacagg tttaatgatg atgatagtag
    23461 ctggtataac agctttatca gctgtgttag tttgtaaagt atcagcaaat ctttcatctg
    23521 gattttgtag agatattaga gaagcagtat ttataaaatc acaagattta tcaataaatg
    23581 attttaataa tataggtaca gcatcaatga ttactaggag tactagtgat attactttaa
    23641 taggtcagtc tgtgtttatg tttattcaat tagtattacc agcaccaatt ataactgttt
    23701 ctggtctatt cttagcatat tctatagata aagcaatgac aattattatt gttgtagtaa
    23761 tgtttttatt catgctttca gcatttttag ttggtaaaaa gcttataaag ttgtttaaaa
    23821 tgatgcaaat aaagatggat aatatgaata gggtgttacg tgaggtagta actggagtaa
    23881 gagttataag agcttttaat aggagtcgtt ttgaaaaaaa gagatttgat agaactgcaa
    23941 tagattattc tgaaacagca atttcaataa ataaaatatt tgcagtactt atgcctattg
    24001 taatgttaat aatgaattta ggtatagtaa gtattatttg gtttggtggg atgagagtca
    24061 gtaatggaaa tatggaaata ggtcatataa tggcacttgt tgaatattgt atcttaattt
    24121 tattctattt aatcatggga gtaatggtat ttatgtacat acctagagca ggtgcatgtg
    24181 cagatagagt aaatcagata ttagatatag aacctgaaat tgtagatgga aatggtcata
    24241 aagatactgt ctcagagaga ggacatttag tatttaaaaa tgtaaccttt tcatatgcac
    24301 aatcagaaga acctgtgtta aataatataa catttgaagc aaaatctgga gaagtaactg
    24361 caattatagg aagcactggc tcaggcaaat ctactattgc aaatataata ccacgttttt
    24421 ttgaaattca atctggagaa atatctataa atggtcagga tattaagaag attcctcaaa
    24481 aagaacttag agataagatt ggatttgtac cacaaaaagc ttttttattt agtggaacaa
    24541 tagaagaaaa tataaggtat ggaaaagaag atgcaagtat tgaagaagta aagcatgctg
    24601 caagtatagc tcaagctgat gaatttattt atgatatgga agacaagtat gactcatttg
    24661 tagcacaagg tggaaataat ttgtcaggtg gtcaaaaaca gagaatatcc attgcaagag
    24721 cgttagttag aaagcctgaa gtatatgtct ttgatgatag tttttctgca ttagatttta
    24781 aaacagataa aagacttaga aaagcgttga aaaatgaaat caaagattca tcagctataa
    24841 ttatagcaca acgtataagt acaatcatgg atgctaatca gattatagtt ttaaatgatg
    24901 gaaaaattgt aggtattgga aaacataaag atttacttga aaattgtgaa gtttataagc
    24961 aaattgcgga ttcacaactt agtaaagaag aacttgcata aaatgaagaa ttaatctgat
    25021 atttggaggt gtatgatatg agtgaaaaaa gaatagatga acaagatata gaagtactta
    25081 gttttgaaga tggcatgtca gcagaaaaag ctaaggatgc aaaaaaaact actaagagat
    25141 tattaaaata tatggcaaaa caaaagttta aaatattaat tatttttatt tcagtattga
    25201 taagttcagc gcttacagtt ttagcaccta tggttatggg aaaagctatt gaccaactat
    25261 ttaatggaat aaaaactgca gtccaaacag gtacaaaatt cagtgtgaat tttagtacta
    25321 tgggagggat agtatcaata cttctagggt tgtacttaat tagtgcagta tttatatata
    25381 ttcaacagta tattatgtca ggagttgcac aaaatctagt tcttagcatg agaaaagatt
    25441 taagtgataa actcaataaa cttcctctta agtactatga ttctcataaa aagggggaaa
    25501 cacttagtat agtaacaaat gatttagaaa aagtagcaga tagtttacaa gaaggattaa
    25561 tgcaacttat tacagcagta gttacagtag ttggttctat tgtaatgatg ataagtataa
    25621 gtgtaccact tacaatagta tcagctataa cattacttgt ttcattgggt attactgttg
    25681 taattgcaag aaaatcacaa gaaagattct ctgaaaatca aaaggcacta ggagagttaa
    25741 atagcaatat agaagagata tttactggtc aaatagtggt taaagcattt agtaaagaaa
    25801 aggatactat aagaaacttt aaaatattaa atcaaaatct ttataatgca agtagaaaag
    25861 cacagttttc gtcatatgca atatctccaa ttattagatt tataaaccta attggctatg
    25921 ttattatagc agttgtaggt gggatatttg catcaactgg agcaatgact cttggtagta
    25981 tacaagcgtt tattcaatat gtaaatcaag catcagagcc tactacagaa atatcttata
    26041 ttgtaaatat gcttcaagca gcaatagcat ctgcagagag agtttttaca gttatggacg
    26101 aggtggaaga gataaaggat gaagaatctt caaaggttat tagcatgcct aaaggtaaag
    26161 tacaatttga gcatgttaaa tttggatata gtgatgattt tattcttatg aaagatatta
    26221 atatcaatct aaatgcagga gataagattg caattgttgg acctacagga gctggaaaga
    26281 caactcttgt aaatttactt atgagatttt atgagataca aggtggaaga attactattg
    26341 atggtgttaa tataaaagat ttaaagcgtg gagaacttag aactatgttt ggtatggttc
    26401 ttcaagatac atggctgttt aatggaagta taaaagaaaa tattgcatat agtaagtcag
    26461 atgccacaat ggatgaaatt gtaagagcag caaaatcagc aagagtagac cattttataa
    26521 ggactttacc acaagggtat gacactatat tgactgagga tgcttctaat ctatctcaag
    26581 gtcaaaagca acttctaaca attgccaggg caatactttc agacccatca gtattgattc
    26641 ttgatgaagc aacatctagt gtagatacta gaacagaggt agaaattcaa aaggcaatga
    26701 ataacttgat gaagggaaga actagttttg ttattgcaca tagattatca actattcgag
    26761 atgctgattt aatattagta atgaaagaag gaactattat tgaacaagga agccataagg
    26821 aacttattga aaagaaagga ttctatgaag aactttataa cagtcaattt acaagtgaat
    26881 atgatgaaga tgttgtatga ttatcaagtt ttaggcaact aaatgattga taaatattaa
    26941 gttaaatata gtaacatgta aggttatatg gaataagagt atttctctat ttcatataac
    27001 cttttttatt tatcaaaata agataaaatt tcctttctaa attagattga agaatgtatt
    27061 tatcaatata aaagtaaaat aatcattatt attagtgaag taaatattgt ttgccacata
    27121 tatgccataa agatgcactt attttacgga gatttaacaa agtagtgtaa agtatactct
    27181 aaatataaaa taaatcaaat agagttaaat aaattgagag gtgatataaa tagtgaataa
    27241 aaaattgaac tatagatttg aaatgaagca caaaataact gaagcagatg tgttagcttt
    27301 aaaatcaaga ctctatccaa taatgaagaa agatgaaaat gcatctaaag atggaaaata
    27361 tcttattagg agtttatatt ttgatactcc tgaagataaa gcacttttgg ataaattaaa
    27421 tggcgttgca ataagagaaa agtttaggat aagattttat aataatgact gctcttatat
    27481 aaggttagag aaaaaaatca aacattacaa catgacatca aagctatcaa ctaatttaac
    27541 caaaaaagaa gtaataaata tactaaataa tgatattgaa tttctaaaag agtcgacaaa
    27601 ttgtttatta agagaatttt atttgaaatt aaaatgtgaa agattagagg caaaaagcat
    27661 agttgattac aatagagaag cttttattta tccagttgga aatgtaagag ttacaatcga
    27721 tagtgatatt aaaacttctg taaattcggt agatttattt aataaagatt tgccaactat
    27781 ttcggtaatt gatgagaata tgactgtatt ggaggttaaa tatgatgaat ttataccaga
    27841 ttttataaag gatttaattc aaattaataa gactacctct acagcagttt caaagtatgc
    27901 ttcaagtaga ttatatattt aagaataaag gctatgattt atataaagat atacataaat
    27961 aaggttaaaa aacaaataag ttaaaaataa ataaaataaa aaataaataa gttaaaaata
    28021 ggaaggatga ttttaattat gacatttaat gatattttta aatcaagttt tatagaaaac
    28081 gtatcaggtt tttcatttgt ggacagtgct ttagcactag gttcagcttt tttagtaggt
    28141 ttgtttatat atatggtcta taaaaagact tatatgagaa ttatgtattc tcgaccattc
    28201 aatgtgtcgc ttgtagcact tacaatgctt acaacttttg taatattggc ggttacttca
    28261 aatgtagttt tatccttagg tatggttggt gctttatcta tagtaagatt tagaacagca
    28321 attaaagacc caatggattt agtatttcta ttttggtcat taggttcagg tatagtttta
    28381 ggagcaggtt tgattccctt agctgtcatg ggttcaataa ttatgggatt gattctaata
    28441 ttcttttcaa ataaaacaat atctgaaact ccatatatat tgatggtaaa ttgtaataat
    28501 gaagattcag aagatatagc aactgataaa attaaaaaag tatttactaa atatcaaata
    28561 aaatcaaaaa gtgttacacc agaaaaagga atagaattag tgtttgaagt aagaatgaaa
    28621 gatggagaaa caagtcttat aaatgacttg agtcaagtag atggagttac taatgcagtt
    28681 cttgtaagtt ataatggtga ctatgtagca tagagaggag taatctttta tgaaagataa
    28741 aaaatttacc cttcttatct cgattatgat tatattttta tgtgctgtag ttggagttta
    28801 tagtacatct agcaacaaaa gtgctgattt atatagtgat gtatatattg aaaaatattt
    28861 taacagagac aaggttatgg aagttaatat agagatagat gaaagtgact tgaaggatat
    28921 gaatgaaaat gctataaaag aagaatttaa ggttgcaaaa gtaactgtag atggagatac
    28981 atatggaaac gtaggtataa gaactaaagg aaattcaagt cttatatctg tagcaaatag
    29041 tgatagtgat agatacagct ataagattaa ttttgataag tataatacta gtcaaagtat
    29101 ggaagggctt actcaattaa atcttaataa ctgttactct gacccatctt atatgagaga
    29161 gtttttaaca tatagtattt gcgaggaaat gggattagcg actccagaat ttgcatatgc
    29221 taaagtctct ataaatggcg aatatcatgg tttgtatttg gcagtagaag gattaaaaga
    29281 gtcttatctt gaaaataatt ttggtaatgt aactggagac ttatataagt cagatgaagg
    29341 aagctcgttg caatataaag gagatgaccc agaaagttac tcaaacttaa tcgttgaaag
    29401 tgataaaaag acagctgatt ggtctaaaat cacaaaacta ttaaaatctt tggatacagg
    29461 tgaagatatt gaaaaatatc ttgatgtaga ttctgtcctt aaaaatatag caataaatac
    29521 agctttatta aaccttgata gctatcaagg gagttttgcc cataactatt atttatatga
    29581 gcaagatgga gtattttcta tgttaccatg ggattttaat atgtcatttg gtggatttag
    29641 tggttttggt ggaggtagtc aatctatagc aattgatgaa cctacgacag gtaatttaga
    29701 agacagacct ctcatatcct cgttattaaa aaatgagaca tacaaaacaa aataccataa
    29761 atatctggaa gagatagtaa caaaatacct agattcagac tatttagaga atatgacaac
    29821 aaaattgcat gacatgatag catcatatgt aaaagaagac ccaacagcat tttatactta
    29881 tgaagaattt gaaaaaaata taacatcttc aattgaagat tctagtgata ataagggatt
    29941 tggtaataaa gggtttgaca acaataactc taataacagt gattctaata ataattctaa
    30001 tagtgaaaat aagcgctctg gaaatcaaag tgatgaaaaa gaagttaatg ctgaattaac
    30061 atcaagcgta gtcaaagcta atacagataa tgaaactaaa aataaaacta caaatgatag
    30121 tgaaagtaag aataatacag ataaagataa aagtggaaat gataataatc aaaagctaga
    30181 aggtcctatg ggtaaaggag gtaagtcaat accaggggtt ttggaagttg cagaagatat
    30241 gagtaaaact ataaaatctc aattaagtgg agaaacttct tcgacaaagc aaaactctgg
    30301 tgatgaaagt tcaagtggaa ttaaaggtag tgaaaagttt gatgaggata tgagtggtat
    30361 gccagaacca cctgagggaa tggatggtaa aatgccacca ggaatgggta atatggataa
    30421 gggagatatg aatggtaaaa atggcaatat gaatatggat agaaatcaag ataatccaag
    30481 agaagctgga ggttttggca atagaggagg aggctctgtg agtaaaacaa caacatactt
    30541 caaattaatt ttaggtggag cttcaatgat aataatgtcg attatgttag ttggtgtatc
    30601 aagggtaaag agaagaagat ttataaagtc aaaataagat taaaattgaa tataaataat
    30661 tttaatactg ttaagtctaa atatttactt agcagtattt ttttagaaaa taatgtattt
    30721 ggttaataat ataaggtaat aaattgataa aaattaaata cagcatatta ataatacata
    30781 ataaagtaat aaaaaaactg tttaagtata ataacttata tttagacagt ttttttcatt
    30841 tataaataat ataaatgaaa ttgaaaacat ttgaaggata gaaattggtt ggataaatgt
    30901 ataaattact tcaccaaaaa ataatgacaa aaattgtaac taagttgtaa taaaatggta
    30961 gttatttagc ataaaataat gtatcataga agtatattat aaaattaata aaataagatt
    31021 tttgtaagaa aactgctggc ttttgatatt tagagtgagt agttaaatta tgtttaggag
    31081 gggtagaatt tgttgcaaaa agtattatgg aaatttttaa agctagtagt cggtttattt
    31141 atatgttcag ttggtatagt attgacgata aattgtaacc ttggattatc tccctgggat
    31201 gtatttcatc aaggactttc aaatcatata ggaataacta taggaactgc ttctataatt
    31261 gtaggctcta tagtagttat tgctgatgtc gttcttggag aaaatgtagg atggggaact
    31321 gtttttaaca tgctactaat tggattcttt atggatttac ttttatatag taatttaatt
    31381 ccagaggctg atagtttatt tgtaggaata attatgttga tattaggatt ggttcttttg
    31441 tctgttggaa tggtatttta tatgggaagt ggtttaggaa gtggacctag agatggtttg
    31501 atggttgcaa ttcaaaagaa aacaggaaaa tcacttaaat taataagagg tactatagaa
    31561 gttggagcat taatagtagg atttttatta ggtggaaaag ttggtatagg aactataata
    31621 agtgcatttg gattggggta tttcacacaa atggtatttg gcttatttaa attagattgt
    31681 agtaagatta aacatagatt tatagtagat gatataaaat ttatcaaagt gtatatgagt
    31741 ggtgagaaaa aagctgctgt tcaaaatata gttaagtaat ttaagaataa attcaacgaa
    31801 atgttaagta ttttaaatta acattttgtt tagatagata aattgaatat taaaagaata
    31861 ttaaaatatg aggattgcta gaacactata aaatatagat ttctagcaat tttttatttg
    31921 ttcaatattt ttttagtact agaagtctta ttctctattt aatgaatgaa ttaatttata
    31981 attagagata ctaaacttta tagatattaa tataaacacc tttaaactgt gtattttcca
    32041 aagcctttaa aatgcttgta tttattctaa ataaaaaaat aaaaaaatgt caaaaaaact
    32101 cttgtattat atccaaattt tatatataat tacctatgtt gtttagaaat tttaaactca
    32161 atgtttagta aacaaaatgt ttagtaaaat aaaacagaaa ctaggaggtg caacatggat
    32221 ataggtgaaa agataaaaag attaagaaca gaaaaacaac tgacccagga agagttagct
    32281 aatagatgtg aattgtcaaa gggatttatt tctcaattag aaaataactt aacatcacca
    32341 tccatagcta cccttataga tatacttgaa atactaggta caaatttaag ggaatttttt
    32401 aatgaaatag atgatgaaag aatcagtttt acaaaagaag atatgtttga aacagaagat
    32461 gaagatttaa aatataaatt aaagtggtta attccaaatt cacaaaaaaa tgaaatggag
    32521 ccaataataa taacattgta tccaggagga cagtataaag aagaaaagcc acatgaaggt
    32581 gaagaatttg gatatgtatt agcaggttca atatatgtcc atataggcga aaagaaaaat
    32641 aaagtaaaaa aaggagagag tttttacttt agaccgaaag caaatcacta tatatcaaac
    32701 gagggcaaaa ctacagcaaa agtgatttgg gtaagtacgc caccatcatt ttagaataga
    32761 ggtgtttaat aattggtaga aaatataata gaattaaggg atatatctaa acattatgaa
    32821 gaacttactg ttttagataa tttcaattta gatataaaga aaaatgagtt tctaacatta
    32881 cttggaccaa gtggatgtgg aaaaactaca acattaaaaa taatagcagg atttgagtat
    32941 gctgatgatg gtaaagtttt atttgaaggt aaggagataa ataatttgcc accatatgaa
    33001 agacaagtaa acacagtatt tcaaaaatat gctttatttc cacacatgga tgtttatgaa
    33061 aatgtagctt ttggattaaa aattaaaaag actccaaaag atgtaataga tgctaaagta
    33121 aaagaggttt tgaaacttgt tgcactagaa ggttttgaaa gacgacaagt tgaatcattg
    33181 agtggaggac aacaacaaag aattgcaata gcaagagctc tagttaatga accaaaggta
    33241 ttattactag atgaaccact tggagcactt gatttaaagt taagacaaga gatgcaaata
    33301 gaattaaaga gaatccaaaa aaaattagga ataacatttg tttttgtaac tcatgaccaa
    33361 gaagaggctc ttactatgtc agatactatt gttgtaatgt ataagggcaa aattcaacaa
    33421 atgggaacac cacaagatat atacaatgaa cctaaaaatt catttgtagc taagtttata
    33481 ggtgaaagta acatatttga tggaattatg atagaagatt ataaagttaa tttctgtaat
    33541 agagattttg agtgtgttga taaaggattc gaaaaaaacg aaaatataga agtagtaata
    33601 agaccagaag atataaaaat ggtaaagcca gaagaaggaa tgttaaaggg gaaagttaca
    33661 tctacagtgt ttaaaggagt gcattacgag atagaattga atgaaaatgg aagattgtgg
    33721 atacttcata atactaaaaa tgctgaggtt gggactgaac taggcatgga tatatatcca
    33781 gaagatatcc atataatgag aaaagagaat aactaagatg aaaagaaaat cttttttagc
    33841 atatccatat gtagtttgga gtgcgatatt tgtaataatt cctttaatat tagtagtatt
    33901 ttttagtttt acaaaagaat caggtggagg ttatgccttt acacttgaga attataaaga
    33961 agtaattgac cctatatata tgaaagtttt tgggcgttct attcttttag caggaggagc
    34021 tacattaata tgccttatag ttggttatcc agtggcatat ataatttcaa aggctcgtgt
    34081 aagtagaaga ggttcattaa tattattgtt tattcttcct atgtggatga actttctttt
    34141 aagaacatat gcatgggttg ctatattagg aaaaaatgga cttttaaata catttttagg
    34201 ttggtttgga atacaaccat tagcaatttt gtatactaat tttgccatac ttttaggtat
    34261 ggtgtataac tttttaccat ttatggttct tccaatatat acagcactat caaagatgga
    34321 taatgatttg ataaacgctg ctcatgactt gggtgcaaat aatatgacag tttttagaaa
    34381 aattatattt cctttgagtt tgccaggtgt aatgtctgga ataactatgg tatttatgcc
    34441 agcagtcact acttttgcaa tatcaagact gttaggtgga ggtaaaataa tgctggttgg
    34501 agatttgatt gaacaacagt ttacagttgt tggagattgg aactttggtt cagctatatc
    34561 gatatttatg atgatagtaa tattaatatc tatgtctata atgtcaaaat ttggagatga
    34621 atctgacaaa gaaggcggtg ggttactatt ttaaaattaa agaaaataac ttctaatata
    34681 tatttagcat tagtattctt atttttgtat gcacccatat ttgctttggt actattttct
    34741 ttcaatgatt caaaatcaat ggcaagatgg aatggattta catggaagtg gtatggtcaa
    34801 ttacttcaaa atgagagtat aatgagtgct ttatattata ctattgtaat agctatatta
    34861 gcgtctgtaa tatctacaat agtaggaact ataagtgcta taggaataca taagatgaga
    34921 ggtaagagta agaaattaat actaaatgta aactatcttc ctattttaaa tacagagata
    34981 gttacagcag tagctcttat gagtctgttt gtatttgtaa aaatggaatt tggatttaca
    35041 acaatgctat tggcacatat aatgttttgt ttgccatacg ttatactttc agttttgcca
    35101 aaaatgaagc aattacctga taatatagaa gatgcagcta tggacttggg agcaacacct
    35161 atatacgctc ttagaaaagt aattttacca caaataaaac caggtatagt ttctggattt
    35221 ttaattgcat ttactatgtc aatagatgac tttataataa gtttctttaa tgctggaaat
    35281 ggtgtaagca atctttctat agaaatctat ggaatggcta gaagaggtat aaaacctgaa
    35341 ataaatgcat tatcaacaat aatgtttgca gtggttctag gtttactttt actagctaat
    35401 aaaaaagaat ctatagtaag ggggattaaa taatgattaa gtttaaaaaa gcaatatccc
    35461 ttatagctgt aggtatgatg acagttactt tatttacagg atgtaataaa gcaactgatt
    35521 ctacaaaagt tttaaatgta tataatgttg gagattatat agatgagtct cttatagata
    35581 agtttgaaaa agaaacagga atagatgttc aatattcaac ttatgatact aatgaaatga
    35641 tgtatcaaaa agtaaagagt ggtagtacac actatgattt agtatttcct tcagactata
    35701 tggtagagaa aatgaaaaat gaaggtcttt tggaaaaatt agattttaag aatataccta
    35761 atatgaaata tttagataag agttttttaa atcctgtata cgacaaaact aatgaatata
    35821 gtgttgcata tatgtgggga actcttggaa tactgtacaa taagaaagaa gttaaagacc
    35881 ctatggatag ctggaatata ctttggaatc caaagtataa gggaaatata atgatgtttg
    35941 actctgttag agatactata gggataactc ttaaaaaatt aggatatagc atgaatagtg
    36001 taaatccaaa agaaataaat gaagctaaaa atcttttaat gaaacaaaag gatttagtat
    36061 tggcttatgt aaatgatgaa ggtaaggata gattgcttgg tggagaagtg gcaatgggta
    36121 tgctgtattc tggagatgca gttacactaa tggagcaaaa tcctgacctt gattatgcta
    36181 tacctaaaga gggaactaat aaatgggtag atgctatgtg tgtacctaca acagcacaaa
    36241 ataagaaaga agctgagctg tttataaact ttttattaga cccagagaat gcaaaagtaa
    36301 atgctgagta cattgggtat tcaacaccta atacaggagc actaaaatta ttagacccag
    36361 aaataactca gaatcctgtt gcatatccat ctaaagaagt gcttgataaa tgtgaaactt
    36421 ttgaagatat tggtgaaaat ataaaattat atgacaaggc ttggatagag ttaaaatcaa
    36481 aatagggtct tgttggttta agtaaattta cttaataaat acttaataaa actgttcttt
    36541 tttagaaaat ttattttcta attattgaac agttttattt tttgtttaag ttatattcat
    36601 atgcaattta aatagtttat taataaatga aaacttattt gtataatgac tttaataata
    36661 attagaaaaa atgggcaact taatagaata attggaggtg taaatttata aaaaatgaat
    36721 aggataaata aaaaaataga tttattaata ttggctttgc tcgtttttat atttttaatt
    36781 gttggaatag taatactatc gattaggaca gaaaataaat tgaatgtgtt cataatgtta
    36841 gcaataattt tttttataat aatgttaact tatttatcaa attcagtagt ggggttgata
    36901 acaagttcaa taattatatt tatgtataca tcctatattt tatataacaa tattactcac
    36961 aatatggatg ttgaatttat atcatatatg tggataatag caacaccagt atcttcaatt
    37021 ataatgggta atctgaacaa aagcataaat gaattgcaaa atacaaataa aaaactatct
    37081 gaacagtata aggaattagt gactattgat agtgaaactg gacttagaaa tttaaagatt
    37141 ttttataatg atgtaaatat ggagataagt aaatctatta gacataatac tgatttttct
    37201 cttatgatag taaaattacc atactacgga aacctacaga ctatatttgg tgaaaacaaa
    37261 actaataaaa tagtaaaata tattggttca aatattatag aatgtacaag aaatgaagat
    37321 ataatttata gtttacaaaa agatatgata gggatactaa tgccaaatac atctttggaa
    37381 ggttcaaaag ttgttaaaga cagaataaag aaaaggataa aagaattaaa tttagattta
    37441 aacaataggg gaaaatatgt aaatatagat gtcaaaattg catttttaca atataagagt
    37501 agttttggag atagtataaa ttttaagaat atagttgaag aggaacttca atatgatgtt
    37561 taaaatattt aataaaagtc tattaaaatt agtagttgta ttatcaatag tactgacaat
    37621 atcaaatttt agcttatctt catatgcatc agaaattact tccagcacag ataaggtatt
    37681 aatcatatat gattctaaga aagaaacagc ttataataga gatattttaa atattatgag
    37741 gactctattg ggaagattta gttcagatat agagctatta aaattgagta actatgatgg
    37801 tgagataaat aaaaattatt acagtcatat atttatttta ggaatcaatg aaaactcata
    37861 taataatgat aaaaacacta aaaacttgat tagttcctta aatttatata aaggaactat
    37921 atgttggtta ggatatggta tagaaaatct tttggaacat aaaaaataca atttagacta
    37981 tgtaggaaaa acaaacaata tagttagtgt aaattataga ggtaaatcat ataacttgga
    38041 tgaacattat gtttttaaca ttgtagaatc aaaagataca agtaataaag taataggaag
    38101 tattaatgat accttaaata agtatccata tataataaat gataaaaatc ttttttatgt
    38161 atcaaaatta gatttagatg gagttttatt ttatatattt tgcgattcac ttaatgatat
    38221 ttttaatatt aagacgtttg ataaaggacg tatatttgta agaatagaag atgtacatgc
    38281 ttttagagaa ccaaaaaatc ttgtagagat agcggattat ttaagtagta agaatatacc
    38341 atttacgatt gcattaatac cagcatatgt aaatcctaaa aatcataagg taataacttt
    38401 atcagaaagc cctgaaattg tcaaagcaat aaaatatatg caagataaag gtggaactgt
    38461 gatattgcat ggatatactc atcaatacaa gaaagaagaa gtttctggag aagggtatga
    38521 attttgggat ggtaaaaaag atgaaccctt aaaagaaaat atgaagatat ttgtcaaaga
    38581 tagagttttg aatggtctta gagtatgtat agagaatgga atttatcctc ttgcatttga
    38641 agcgccacat tatgcaatgg aatcagaggg atataaagaa ttaaaaaaat atttttctac
    38701 ttatataggg caacatcaaa ataatgataa aaaatttagc acaaatacat atccttatat
    38761 tattagggat acagaggagt ttaatatatt tatacccgaa aatttaggat atatagaccc
    38821 agaagataag tttacttttc aaaatataaa agaaaattta gataaattat ccattgtaag
    38881 aggttttagt ggagggttct tctttcattc atatttaaac atagagtatc taaaaaacac
    38941 aatagaatat ttagaaaaac agaatataga atttatgaat ttaagagatt ttaataattg
    39001 ggtaaaagtt gatgaaatac aaataagaaa taatggagat gaaattatag tcaactatga
    39061 taaggactta gatgaactaa ctaaaagtga tactagattt aaatccatat ccaatattag
    39121 taaagtttta atattcatag tatctataag tgttttaata tttgtgatta tatttatata
    39181 ttttaagaga atagataaaa agaaattttt aaaataaagg attttatatt agaatcaata
    39241 ttttaaaata ttttaggaga aatttatgag agatttaaat gtatcagagt atttgttcgt
    39301 atttagtttg tttagtatat ggtctttatt gcttataaat attattttag ctatgggtgg
    39361 atatatattt tattttaaaa actttgataa agaaataaaa gaaattgatg aatatcctat
    39421 gatatcaata cttgttcctg ctcataatga agctaaagtg ataggtagaa ctgtggagtc
    39481 tttgctatta ctcaattatc ctaagagtaa gatggaactt attgttataa atgacaattc
    39541 ttctgataat agtaaagaaa ttttagaaaa tataaaggat aggtacaaca attataattt
    39601 cactataata aatacagata gtttaacagg tggaaaagga aagtctaatg cattaaatat
    39661 tggatatacg atttcaaaag gagattttat agctgtttat gatgcagata acacaccaga
    39721 taaaaatgca cttaggtatc tagtacaaac tatagtaatg aatgatgaat taggtgctgt
    39781 tattgggaaa tttaggactc gaaataaaaa taaaaaccta ttaaccaaat ttataaatat
    39841 agaaacatta agctttcaat ggatgtcaca agctggtaga tggcaacttt ttaatctatg
    39901 tacaatacca ggaactaact ttatattgag gagaagtatc atagaagaaa ttggaggatg
    39961 ggatagtaaa gcaattgcag aagatacaga aataagtttt aggatatata aattgggata
    40021 caagataaag cttgtaccac aatctataac atgggaacaa gagccagaaa ctgttaaggt
    40081 gtggataaag caacgtacta gatgggcaaa agggaatata tatgtattaa tgaaatatat
    40141 aaaaaatata tttaaacagg gaagaaataa gattgtgttt gatatagctt actttttttc
    40201 tgtatatttt ctatttttaa catctgtaat tatatccgat attttatttg ttttaagtat
    40261 atctaaacta gtagaaattt ctattccaat taactttttt ctaatatgga tactatctta
    40321 tcttttattt attatagaag tgagtataag ccttactata gaaaaaggtg aagcaacaat
    40381 tgaaaatata ttcatagtgg caataatgta ttttacatac tctcaactat ggctgtttgt
    40441 agctataaaa ggaatgatag agtatctaaa agatataatt tttaaaagag aggttaaatg
    40501 gtataaaaca gaaagatttt gattcatgta caggaggaat taacatgaaa aaatttatta
    40561 tatcaattat atctttagta ttgttttttt caaatatatc acttatatac aaagtaaatg
    40621 ctgatgagac taaagttaaa aattataagt ttgaaagaga tataactatt gatggtgtta
    40681 taggaagtaa tagtactttt tttgaagtaa ataaaaattg ggatattgaa gaagttttat
    40741 tgcatcttaa ttttagtaag agccaaatat taaatggaga tgtttcaagt ttaactgtat
    40801 tgataaataa tgtaccaata aaatctatta aattaaatgc taagacaaat tataaaaata
    40861 cattagaagt attagttcca aaagattata taattcaagg atataatgaa ataaaaataa
    40921 aaacatacaa aacaatatct gataaaattt gccaagatga ttccaatact gggaactgga
    40981 tggttattca caaagaatca tatatatcaa ttagatataa gcagaaaaag gtggaaaact
    41041 caataaatga atatccgtat ccatatgcag aaattgaaaa taatcataaa ttagatacaa
    41101 ctatagtagt accagataat atgactagag gagagactac agcagtgttt aatttggcat
    41161 cagcatttgg aaaaataaca aaaaatgatg atttaaagtt agatgttaaa ttatattcag
    41221 aaatgaaaaa ttggtcagat gataatatta tatatattgg taaacctgaa aatacagcag
    41281 aagaaatttt ggatatttta agtataaagg aacaaaccct attatcttca aactgtataa
    41341 tcaagcaagt agattcacca tacaataaga ataagaagat gatggtggta attggtagta
    41401 atgaagatga tttaataaag gcatcaaatt tattaataga aaatagactt tctaaccaag
    41461 ttttatcatc atcagtactt gtaaacaaag agactaatat aaaaattaat agggaacaaa
    41521 aattaaattt aggtcattta acattaaaag atttaggata ttctgacttt ttgttggaag
    41581 gagcatttaa tcaacaagct ttgtttgatg tcaaaatacc aacgggtaag gtattagatg
    41641 atggttcaaa gattatctta aatttaagat attctgacaa tttagatttt gaaaaatctt
    41701 tagttacagt gtctataaat gatgttattg tgggaagtaa aaaattggat aggtcacatt
    41761 ctaataatga taagttagaa ttaaaaatac ctaaagatat agataataaa aactattatc
    41821 aagttaaatt gacatttaat ttgagtataa aaaattcgaa ttgtgttact agagagagta
    41881 ataatccttg ggcatatgta tcaaataatt cttatttggc gttatctacg aaagaaaatg
    41941 aaaccttatc ttttgaaaat tatccgtatc catttgttag agatgatgaa tttaatgatt
    42001 taactgtgat aatgccagat tattcagggt cacaagctat gacctggatg tttagattag
    42061 gtgttacttt aggtgcgaat ataaattcac ataatggaaa tataaatgta attagaggaa
    42121 aagaatttag tgacaagtat aaggatacta atattgtagt ttttggagtg ccacataata
    42181 attctgttat aaagatgtta aataataatt taaatataaa gttcgataaa aattactcca
    42241 attttatatc aaatgataag ataagcttta ttgatgatta tggaaaaaat atttctacta
    42301 ttcaattaat aaaatcacca tataataatc aaaaaaatat catggtaatt agttcaatga
    42361 atgaaaaaaa cttatattta ggaatggatt atttgttgaa taaaagtaaa gttaatgatt
    42421 taaaaggaga tacattgata attgatgaat acggagaggt agaagattta gcttataatt
    42481 taaaatctaa gaaagaagta aaagattcaa gttggaatat gagtataaat aagacaacta
    42541 aagtattttt aatgatttct tttattacta taattatagt cat
//
LOCUS       DAMJVK010000029        39610 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.029, whole genome shotgun sequence.
ACCESSION   DAMJVK010000029 DAMJVK010000000
VERSION     DAMJVK010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 39610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39610
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.029"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            154..1467
                     /locus_tag="PC414_001583"
     CDS             154..1467
                     /locus_tag="PC414_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF2463981.1"
                     /translation="MHTQLQKTIGLSAALSTVVGMVIGSGVFFKPQAIYTTTNGAPGL
                     GIIAWLLGGFITITAGLTATEISAAIPKTGGMMIYIEEIYGEKLGFLTGWMQTVLFFP
                     GTSAALGVIFAQQASELLGMSPNNMANVLPIAIGVILFLALLNIIGSSLGGKVQTVAT
                     IGKMIPLILIIVFGFIKGQSSEVLNPFVGDGVNASNALGQVLIATLFAYDGWINVGAI
                     SGEMKSPEKDLPRAIVGGLSLVMAVYIIINIAYLWVVPASELATVTSPATLVATRLFG
                     NIGGKVITVGILISVFGTLNGYLLTGSRIPYTLAEMGTLPASKTLLKVNSGGSPVNSI
                     LLITVLACVYALSGQFNLLTDLTIFSIWVFYVLTFIGVMRLRRQKPDLHRPYKVPLYP
                     IIPIIAILGGLFVIINQILTSTVISLGGIFITLLGLPVYYYMKKR"
     gene            1639..2814
                     /locus_tag="PC414_001584"
     CDS             1639..2814
                     /locus_tag="PC414_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HDF2463982.1"
                     /translation="MLGLYVHIPFCVKKCKYCDFNSYKMDIDSKKRYIEDLKIEMELY
                     SNKLYKDNKYKNKECCSLNKNDKITSIFVGGGTPSILTSDEIREVFISIKEMFDIDEN
                     AEITIECNPGTLTLEKLKTMKEIGINRLSIGLQAIQEKHLNFIGRIHTYEEFEKNYKD
                     ALSVGFKNINIDLMYSLPNQTLCDWKETLEKVVHLNPTHISAYSLILEEGTELYNMYE
                     SNKFELIDENVDIEMYEYTINYLKSKGYNQYEISNYSKEGYNCEHNILYWECEHYIGI
                     GAGASGYINENRYNNVESLEDYHLSLVKREKPIQENEILSEKDMIEEKIFMGLRMNKG
                     IKFEDFKKKFGIDFREKYNKQIEMLLARKLINQSFEGIQLTQKGREISNSVFIEFME"
     gene            2917..3936
                     /gene="hrcA"
                     /locus_tag="PC414_001585"
     CDS             2917..3936
                     /gene="hrcA"
                     /locus_tag="PC414_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcription repressor HrcA"
                     /protein_id="HDF2463983.1"
                     /translation="MELNERKLNILKAIVKDYIETAEAIGSRTISKRHDLGVSAATIR
                     NEMADLEELGYLIQPHTSAGRVPSEKGYKLYVNSLMSKSELDDNDKILIEQCMNHNIN
                     HIKELIHETSKLLSQLTNYTTVAVTKSLINQSVIKHIQLVAMNDNNILLIVVTDKGDL
                     KKANLTTNVYLDQSKLNLISDNLTRKLLGKSITDLDDNLIAFIKYEISEYSGLIDELL
                     NALNSNMKEEDFSLSLNGATNIFSYPEFNDVLKAKSFLNMLEKKETIADIIKSKGIQK
                     DNLNIIIGSDNDCELAQDCSIVTATYNVDRDLVGRISFIGPTRMDYARIYSIINYMSL
                     LINRK"
     gene            3966..4586
                     /gene="grpE"
                     /locus_tag="PC414_001586"
     CDS             3966..4586
                     /gene="grpE"
                     /locus_tag="PC414_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HDF2463984.1"
                     /translation="MDKKNEQQEVREENDTSINQESETQVELEEEVVNEECETSSEKT
                     DEKEVDDENVTDINSKLAEKKLQDELDELNDKYQRLQAEYANYRRRTQQEKETIGVFA
                     NEKIITELIPVIDSMERALDACEDKEDTMYKGISLVHKQLIDTLVKFGVEEIEAESKE
                     FDPNLHLAVMQESVDGVEANQIVMVLQKGYKLGTKVVRPSMVKVSC"
     gene            4678..6525
                     /gene="dnaK"
                     /locus_tag="PC414_001587"
     CDS             4678..6525
                     /gene="dnaK"
                     /locus_tag="PC414_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HDF2463985.1"
                     /translation="MGKIIGIDLGTTNSCVAVLEGGEAQIIANSEGMRTTPSVVAFTK
                     DGERIVGEPAKRQAVTNADKTITSIKTHMGTDYKVNIDGKSYTPQEISAIILQKLKSD
                     AESYLGQTVTEAVITVPAYFTDAQRQATKDAGRIAGLDVKRIINEPTAAALAYGMDKL
                     DQEKKILVFDLGGGTFDVSILEIGDGTFEVLATAGNNRLGGDDFDQIVIDYLAEEFKK
                     AEGVDLRNDKMALQRLKEAAEKAKKELSSTMSSNINLPFITATAEGPKHLNIDLSRAK
                     FEELTRGLVEKTMEPTKTALQDAGLSTGDIDDVLLVGGSTRIPAVQEAVKKFIGKEPH
                     KGINPDECVAAGASIQAGVLAGDVKDLLLLDVTPLSLGIETMGNVMTKIIERNTTIPT
                     KKSQIFSTAADNQTAVDIHVLQGERSMAYDNTTLGRFQLTDIPPAQRGIPQIEVTFDI
                     DANGIVNVSAKDLGTGKEQKITITSNTNLSEAEIEQKIKEAEMNAEADKQKKEKIEAF
                     NQAESTIYQTEKTLNELGDKISSGEKEDIEKAIADLKAVKDNQDATAEELKKATDEVM
                     TKFQKVSQEMYQKAAQEQQAAQGAEQAQDNGPKDDNVVDADFKEVDEDK"
     gene            6736..7890
                     /gene="dnaJ"
                     /locus_tag="PC414_001588"
     CDS             6736..7890
                     /gene="dnaJ"
                     /locus_tag="PC414_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HDF2463986.1"
                     /translation="MSTKRDYYEVLGISKGAEAQEIKKAYRKLAMKYHPDRNPGDKEA
                     EEKFKEINEAYEVLSDDTKRKTYDQFGHDGLNGQGGFGGQGGFGGQGFGGFEDMFGDI
                     FGDMFGGSFGGGRARRRGPQRGADIRQSVTISFEEAAFGKKMSIKVNRSEECEECNGT
                     GAKPGTSKKTCSTCNGTGQVRTVQRTPFGNIASSRPCSACNGTGEVIESPCSKCHGTG
                     NTRKVKTIEVDIPAGIDDGQMIKLSGQGEVGEKGAPRGDLYIVVNVKSHPLFTRDGND
                     IYFEMPITFVQATLGDEIEVPTLDGKVKYSVPEGTQTGTVFRLKEKGIPRIRGNSRGD
                     QYVKVVVEIPKKLNDKQKELLREFAKECGSNVHEKKKTFGQKIEDMFKKK"
     gene            8856..9791
                     /locus_tag="PC414_001589"
     CDS             8856..9791
                     /locus_tag="PC414_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDF2463987.1"
                     /translation="MYYIGVDIGGTGIQAGVVDNYGKIIFRSECKTVIEKGFEGILND
                     IKIMIYKLLEDNKLTMSDIKSIGFGVPGFINKEGLVTCVNLKWNKKAFNKELKRRFPD
                     VEIHGENDATVAALGEAKFGSMKGANVGVLYTLGTGVGGGIVINQKVFSGAHGLGSEI
                     GHQIIGENYFNCNCGNNGCVETFCSATAIIKYSQKLIEEGEKSRILDLAEGNLENVNA
                     KMVFDAYRENDLVAIKVINRFKEYLAKTFANTINSLDPEIISIGGGISKSSDIILDGI
                     EDLVRKFVLYKTEDIATITCATLGSDAGIIGAAFL"
     gene            9827..11200
                     /locus_tag="PC414_001590"
     CDS             9827..11200
                     /locus_tag="PC414_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2463988.1"
                     /translation="MGKTNARRTLFLIAIGSGTMLNPLNSSMISLALHSIQNDFHISF
                     TTVSWIVSAFYLASAIAQPVSGKIGDLIGRKVLFLSGLMLVILSASIVPLVQSFFILI
                     FIRVIQAIGTSTLYPSGVALIQNNIKERQSSALAVLTIFASTTAGLGPTLGGLLLDLG
                     GWHAIFLVNIPVVLVSICLGYFLFPKEVKEKKSIKETLKNVLSRLDIIGILLFSIAMI
                     FILLFLLSMKESFNLEQLVFGIILMCLFIWHELRTKLPFIDIKLFVSNPKLSKVYLQF
                     IILNFFNYILFFGLPSYFQDALHYSAKSTGLFMLFMSGIGIFISPLTGKWIDKSGTRF
                     PLVTSSIFMFISAVLLSVVFIHIPVIGKGIILSLAGISYGVGNVALQSSMFEESPRDS
                     IGTASGLFQTSRYLGSILATVLLGMVFEVNITSEQFQILGYVMVVLGTISFLLNFILK
                     KELRKVE"
     gene            complement(11388..12155)
                     /locus_tag="PC414_001591"
     CDS             complement(11388..12155)
                     /locus_tag="PC414_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2463989.1"
                     /translation="MEELKDFLERQLNKKINIYPYENQKLDASSHLVTFNNAIYVIDF
                     LDKNNLDNLKGNFYLIYQSHIDFEYLKNIFYNLFEDINIIQHNGFFIVNSKYNLDINV
                     TTQNIIETETYQSTYIFYLGELDSKADFDFRLQLCSDLLPHIIKDNAENKFLNLFDFI
                     RYKTLDLINEDNILNKLIDFNKIKSIDEELLYTGIKFINNDLNISKTSTSMFLHRNTL
                     VYRLEKINEILGFDLKNFENAMIFYLSVKSYFLYKKI"
     gene            complement(12185..13294)
                     /gene="ugpC"
                     /locus_tag="PC414_001592"
     CDS             complement(12185..13294)
                     /gene="ugpC"
                     /locus_tag="PC414_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HDF2463990.1"
                     /translation="MSEVILKNISKLYSNGFNAVKNINIDIKDKEFIVLVGPSGCGKS
                     TTLRMIAGLEEISEGELYIGDKLVNNIEPKDRDIAMVFQNYALYPHLSVYENMAFALK
                     LRNLPKDEIDKKVKEAAKILDLLPLLNKKPKTLSGGQRQRVALGRAIVRNPKVFLMDE
                     PLSNLDAKLRTAMRTEITKLHQQLGTTFIYVTHDQVEAMTMADRIVVMKDGVVQQIAT
                     PQDVYDYPANIFVAGFIGAPQMNFIDVILIEENDEIYAQNEHIKLKLNKEKHYDLIKD
                     NYINNEVVIGIRPEDIHVEDTFIKSNPDTCFKSRIEIKELMGAETYAHLKLGENTITI
                     RFDSKNRINVGDDLTLSVDNSRVHIFDKETTLAIR"
     gene            13552..14289
                     /gene="cas6"
                     /locus_tag="PC414_001593"
     CDS             13552..14289
                     /gene="cas6"
                     /locus_tag="PC414_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HDF2463991.1"
                     /translation="MSRISIFMESCNGKFPNENSMLSVSFIKNILNAENKDFAKNIFN
                     YGEERKNNKQIKDINTAIYIPNLIRSKNELLVDGDIIFNISFYNYSMFSKLYNGILKV
                     KELEYKGYRFKIKNIKIHKEHEIKENGVIFKTMSPIIVKNKEGKYLDVEDSNYIECLN
                     YIANLTLESIRGSGLRKPLEFIPINFKKRVLKEKIRGFKVREYYYINAYAGTFFLKGD
                     MEDLNALYKMGIGYRRTENAGMVDILK"
     gene            14294..15769
                     /locus_tag="PC414_001594"
     CDS             14294..15769
                     /locus_tag="PC414_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2463992.1"
                     /translation="MREIKAKFYRSGDIVRDIGIVYFYELLMDLKNELEKNDYKLKFT
                     CELNRNYLSLESAEYIDPKYISDYILENRLFKVFKNGKKETLEKIFSNIDNLTYVNFI
                     EELDKSSATAKQKEDIKKDFKNRRFPYVRNSGKYGLNTSLENMETNVKRIVDTVIKSN
                     IEIDEIDKLDLTQYEEKDSVCNVCNINKTTKLDIDLRERKDSKYNFLFRGAEKSGFKR
                     SGQVESNICFECEFFNLMCLLYINLKRPMVFAYTNDLRELAFLNHKIMLKRKMYSDKS
                     FYKKLLHEKISSIRLYSFDIDTNKGIILKFDSIIEYKELLKKIELIDIIDNYNFSREP
                     GNTRNLGKRMIDNGNLSNLKELLLDNISVLKQSTGTSREMDFVSSSYNIGLYIKLCWI
                     VDGGEEYKMKNYMESNLIYSRVGKDLFNKMTDESRRKNFSMKVIQLLKSNDRTQLFQT
                     IMHVLVSNGILIGEGFVEGLMQSSELELNTNVGLFIQEIMK"
     gene            15789..16802
                     /locus_tag="PC414_001595"
     CDS             15789..16802
                     /locus_tag="PC414_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DevR family CRISPR-associated autoregulator"
                     /protein_id="HDF2463993.1"
                     /translation="MNEKRVKGVTLTILTESPVPLSYDQGYGNYTPIKKEQYREKIHA
                     KTSIATITYDLRRMLHQEYGWNLSNIVFGKKGNIYPSIKKNVGCVDENGLETDVFGYL
                     IPLKDKGSISKASPLRIIPFRSLNPYKGSTQLITNRGFLSSEFGRKYYDEKEENEVPR
                     DENFPTSQALAIEETLSDYYVYTITLELDRIGVVEVEDGKLLLPEERKFMSKELREKA
                     VKDILDAITVFTRNIKHSSVLLKPLAVIGGAFDKVVPFFWDDVDYNADSGEINLEGVI
                     ETIESYSLKESNTILAINDRLKISNKKELNKFNLSKYPVKEIKNLADRLEIGEDNMWY
                     LKE"
     gene            16818..17615
                     /gene="cas5"
                     /locus_tag="PC414_001596"
     CDS             16818..17615
                     /gene="cas5"
                     /locus_tag="PC414_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HDF2463994.1"
                     /translation="MKVLQCKLKQPTAHYRDPKVFQNEYISTLNLPSKTTIMGMITYL
                     CDRRLNSDIDIGIIGTHHHRELEFSRGENIDFWNEYIKMGKGKDKEKFLLKGNYYDYY
                     KEHKAQNSILNYEVLKEVELTIFISCKDDEELEFIRKKLESPCKYANLGRKEDFVIPS
                     EKGCFVKEVKLKEVIPMNTRDAIKENIKLKNTYVRVDLRDKENVETIINQGVLIALPH
                     KYKDLEANRDDRVYEFCHYIYVDNDGIYPKNIKVNVCIDTKEVFTWL"
     gene            17642..20050
                     /locus_tag="PC414_001597"
     CDS             17642..20050
                     /locus_tag="PC414_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HDF2463995.1"
                     /translation="MKNIMQKEILAKSYNLRTNQQEQTLVEHIKDLFEVLESILELNL
                     YSDKDVEILKICCALHDLGKINSIMQQKMEINNKISYSCSEEERKKLESDKKSLKKIA
                     RHNIFSGAFLKDILEKMNLSEEDKLYIYKSIMLHHGNYEDYMRLSISKVQEEIYEYIE
                     KGILEKEEFNLKDIESYINSILNVDFKFGEDVLDYDYIDKLSESMFIDSDYNNGQIDD
                     SVLNYRKFKYILYKGMLNLIDHSASSRQKGIKFYNDFTDEEIDNMILNEIYKSQGNLE
                     KNNIEFNTIQKRLRELSGRNVLTVAFTGSGKTAADYRKTFKRKFFLVPNKISAESFYR
                     KNIFQNKNDLDTRSSNDYIGLLHGDINLYSENEDNGEHDFVLTLRDIDLSINFCKPCV
                     IATVDQLLLSMFKFPGYEKIFAAVKNASITVDEVHLLEPKMFLILIYFMQFACKYLDV
                     DFHLMTATLPKSYKEQMINKGIIFQEESNENVTDKGEIVFIESNKEEEICEGKDVKVS
                     FIKEKEIKSIVEDALENKQKILIIKNTIDSVNRTYEFLKENLSDKYGGVDIDVLHSRF
                     KFKDKKEKYSKILNGEGDIWISTQSVEISLDLDFNIIISDLATMDSLIQRMGRCNRNN
                     KYEYGNFYILPSEDKIYDNKLKSTTKNILKDILKTNSIFTMGIRKTILDDYYDNSVVK
                     KYFEENFISCDKEIKNIYGVNKEIFDGLDLIFNFEPYKNIVDSKKEAAKIFRDVDVSY
                     KIILEEDFYKEDRELQQDSIQVSGFIFNRLYYYGLISKVEGYMVLKSSSKFEYNSTVG
                     LILK"
     gene            20491..21438
                     /gene="prmA"
                     /locus_tag="PC414_001598"
     CDS             20491..21438
                     /gene="prmA"
                     /locus_tag="PC414_001598"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HDF2463996.1"
                     /translation="MNNWIEVTIKTTTEAVEPITNILYEQGAGGAVIEDPKDFLFQKK
                     NELDWDYVEEEVFKKNEEDDVLIKTYVSEEKNVMEFVEIIKQKVLGLKDFGIDIGEGS
                     VSLDQVNEADWANAWKAYYKPTKVGQRVVVKPTWEDYAMQEGDLIIELDPGMAFGTGT
                     HETTSMCIRELEKYVNKDSKVFDIGCGSGILAIAAAKLGAKEVVAVDLDEVAVKVAKE
                     NVLENKVEKSVSVMHGNLTDVIKDKADVIVANIIADIIKILAKDVQNFMKEDAIFISS
                     GIILDKVEEVKESLIENGFEIVEVQKLGEWSAIVSKLKK"
     gene            21457..22215
                     /locus_tag="PC414_001599"
     CDS             21457..22215
                     /locus_tag="PC414_001599"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDF2463997.1"
                     /translation="MDRFFVEKNNINLQDKTCTIEGEDVKHISKVLRCKLGEKLEICD
                     KNNNEYICEIMNIDKSIVNLEILEKVDINRESELKVRLYQGLPKAPKMEIILQKLTEV
                     GVEEIILVQTKRSVVKVDDKKEDKKFERWERIIYEAAKQSKRGKIPKLRGVLSFKEAL
                     EDMKKNSVNICPYENERTVSIKHALKKCDSNIDSVGIFIGPEGGFSEEEIEQIQKNNC
                     NVVSLGPRILRTETASVVASTIALYELSDLGGEK"
     gene            22217..23515
                     /gene="mtaB"
                     /locus_tag="PC414_001600"
     CDS             22217..23515
                     /gene="mtaB"
                     /locus_tag="PC414_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HDF2463998.1"
                     /translation="MKKVAFYTLGCKVNQYETEAMLELFEKDGYEQVNSEEYADVYVI
                     NTCTVTHMSDRKSRQYIRRVKKKNPDAIIAVVGCYSQVSPEEILDIEEVNLVMGTNDR
                     RKIVEEIKKINSSKKVSTVDDIMKVKAFEEIEISQTNGKTRAFMKIQDGCDRFCTYCI
                     IPYARGRVRSRDIDSIVDEVKKLANNGYKEVVLTGIHVASYGKDLKDRDIKLLDVIKQ
                     INQIEKIERIRLSSVEPILFTDEFVNEVLKMDKVCPHYHLSLQSGCDETLKRMNRRYT
                     TLEYKTIVDRLRSKMPDVAITTDVIVGFPGETNEEFKKTYEFLKEIELSQMHIFKYSP
                     RKGTPAATMENQVDPQMKHFRSEQLLNLSKVNFNKFATKFIGRELDVLFEQNIEGNKY
                     EGLTSNYIRVVVESDKNIQGQILKVKINDVKDEYVEGILL"
     gene            23558..23908
                     /locus_tag="PC414_001601"
     CDS             23558..23908
                     /locus_tag="PC414_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HDF2463999.1"
                     /translation="MDCIFCKIANGEIPSTKVYEDDRVLAFNDLNPVAPYHILVVPKK
                     HYDSLIDIPDKEMDIVSHIHVVINKIAKEKGFDQTGFRVINNCGSDGGQEVKHLHYHI
                     LAGKKLPNYEAGQN"
     gene            24071..24250
                     /locus_tag="PC414_001602"
     CDS             24071..24250
                     /locus_tag="PC414_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HDF2464000.1"
                     /translation="MSEVRVRENETLDSALRRFKRQCAMSGIMSEVRKREHYDKPSVK
                     RKKKAEAARRKNAKK"
     gene            24280..24723
                     /locus_tag="PC414_001603"
     CDS             24280..24723
                     /locus_tag="PC414_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HDF2464001.1"
                     /translation="MSLKQKLQEDLKSSMKNKDTVRKSVVTLIRASIKQYEVDNRVEL
                     DEDGIIDVIAKQLKQRRDALVEFEKAGREDLIKETEGEIEVLKEYLPQQLSEEELEEI
                     VKSTISEVGATSMKDMGKIMSVIQPKVKGRADGKLINKLVKQNLQ"
     gene            24834..25331
                     /locus_tag="PC414_001604"
     CDS             24834..25331
                     /locus_tag="PC414_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HDF2464002.1"
                     /translation="MIISVKEIRNFLINIFIPLIIGYLSSILTMIISGTDISTYYLQL
                     TKPSFAPPAFIFPIVWTILYILMGISSYLILRKGYNLPKVRDAIFYYGLQLVLNFIWS
                     ILFFGFGLRFTALIDIIIMILVSIIMISKFSKIDKRSGYINLIYLMWLVYAGFLNYFI
                     WFINK"
     gene            25524..26087
                     /gene="raiA"
                     /locus_tag="PC414_001605"
     CDS             25524..26087
                     /gene="raiA"
                     /locus_tag="PC414_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDF2464003.1"
                     /translation="MQIIVSGRQMKLTDGIKGYVDGKLSRLEKYLDPESEVKVTVSAK
                     KDRQKVEVTIIPINGQIIRAEDVEDDLYAAIDIVCDKLSRQVVKYKTKVKDKVQNNKS
                     IRFENLDFIDNSSEFDDYDYDDEEDENIVIERRKKFNVKPMSSEEAILQMELVGHNFY
                     MFRNQDNFEINIVYKRKAGGYGLIEQD"
     gene            26300..26536
                     /locus_tag="PC414_001606"
     CDS             26300..26536
                     /locus_tag="PC414_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YabP/YqfC family sporulation protein"
                     /protein_id="HDF2464004.1"
                     /translation="MLEISTDLQVNQPVITVTSNTFISIENYLSILEYEVDLIRIKTK
                     VKTIKISGDKLSLKYITDSEIGIKGIIYNVEYVD"
     gene            26549..27892
                     /gene="yqfD"
                     /locus_tag="PC414_001607"
     CDS             26549..27892
                     /gene="yqfD"
                     /locus_tag="PC414_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HDF2464005.1"
                     /translation="MISFIRGYYVIVVEGVGLEQFLNHLIRNGISVYNVTRIKNTKME
                     FHIDRQDIKDFKNVYRGSKFDIKVKQKTGVPFIIKRVYKHKGMWICALVSLFLLMSTS
                     QFVTDVYIQSPEGIKKEALRNELYKVGVRPGVYKKSIDRKEVRDHMMSKFNDVAYLSI
                     NVKGTNIFVTVTKKAESLKSTDQSNYCNVIALKNGIIEKVIPRSGKSVIKSGDIVQKG
                     DVLLNGANTKSIPEVWASTFYESTKKASYVDTVNKKTGEKKNIYTLSFYDKEFTMRKN
                     IKYKDYVVENKEKKLSIGNYTFPIKIKTSTFYETKKVEVKRNKEELKKELSEKALKEL
                     EYIIPASARIIDVKHNFKVNKNMLEYLITVQTSENIAKIYPLSKSEAERFIKEESKPD
                     EGEEEVPSNPEKRPLNDIRNEFDEDNKDNKDQNNSDENNSNQNSNNSQNNNSNNN"
     gene            27831..28946
                     /locus_tag="PC414_001608"
     CDS             27831..28946
                     /locus_tag="PC414_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HDF2464006.1"
                     /translation="MKIIVIKIVIIVKIIIAITTNKIKLKILGGLIQLIIQKKFTIAD
                     GNFERELFGNFDENVKLIEKTLNIDVILREGNIILIGEEKNVDSALKLMNELHQTVSN
                     GKHLDKQSISYSLSLLLEGSEQKIKELEGTIVITQRGKAVQPKTLGQKEYIKLIENND
                     ITFGVGPAGTGKTYLAVAMAVKAFKRDEVSRIILTRPAVEAGESLGFLPGDLKDKVDP
                     YLRPLYDALFEMLGADKFNKYLERGTIEVAPLAFMRGRTLDNSFIILDEAQNTTSEQM
                     KMFLTRLGFGSKAVVTGDVTQTDLPQNKKSGLIQATEILKGVPGIGSIMLTDRDVVRH
                     ELVQRIIRAYEKHDKREEFKKEERKKAKMQEKTFKRK"
     gene            29003..29464
                     /gene="ybeY"
                     /locus_tag="PC414_001609"
     CDS             29003..29464
                     /gene="ybeY"
                     /locus_tag="PC414_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HDF2464007.1"
                     /translation="MDLILDDRQDKLEVSEELIEKIKDIIIECLDYEGYDDNYEVSLS
                     FVDNKEIHELNREYRGVDRVTDVLSFPLLSDDFEDVELEEESLGDIVVSLERALEQSI
                     EYNHSFEREVCFLICHSMFHLLGYDHDTDENTKEMREKEEHILNKLNITRE"
     gene            29470..30189
                     /locus_tag="PC414_001610"
     CDS             29470..30189
                     /locus_tag="PC414_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDF2464008.1"
                     /translation="MKPEKTRQGIIKAFNAAIEGILYTFKFERNMKIHYLGSVAVLII
                     SLFFNFSKLEMIMLLMSICLVVVAEMFNTAIEKAVDLVTDEYHVLAKIAKDVAAGGVL
                     VAALNSVVVGYILFYDKLTDISGILIYKIRESELHITLICILLVLIAVVVVKALTSTG
                     TPLKGGMPSGHAALAFAIATAITLMTERVVASTLAYIMAVLVAQSRIEGKIHTFWETI
                     AGALLGVLIAILVFQLGMFYN"
     gene            30308..30709
                     /locus_tag="PC414_001611"
     CDS             30308..30709
                     /locus_tag="PC414_001611"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDF2464009.1"
                     /translation="MDNIELLRLAEDARQHSYAPYSGFRVGAALLTKSGKVYTGCNIE
                     CASLGGTNCAERTAIFKAISEGDKDIYKIAIASDNSENNEQTYPCGICRQVIIEFGSD
                     IKIITGYTKGEVFEHSIKDLLPNYFSGNDLK"
     gene            30773..31666
                     /gene="era"
                     /locus_tag="PC414_001612"
     CDS             30773..31666
                     /gene="era"
                     /locus_tag="PC414_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HDF2464010.1"
                     /translation="MFKSGFVSIVGRPNVGKSTLMNNVVGEKIAIMSDKPQTTRNTIQ
                     AVYTDEEMQIVFLDTPGIHKPKNKLGEFMVKAATEAFKNVDLILFVVDDSKKIGPGDR
                     KIIEDLRSVKTPIILVVNKIDQLDQKDELFDIIKMYDREGIFKEIVPISALKGKNTDT
                     LIKVIQNYLEEGPKYFPDYMITDQPERVLIAELIREKVLHYLNDEIPHGVAVEIEKMK
                     ARNDKEIVDVSAVIYCERDSHKGIIIGKNGRKLKGIGKSARQDIELLLGSQINLQLWV
                     KVKENWRNLQNYINNFGYNDK"
     gene            31678..33057
                     /gene="mgtE"
                     /locus_tag="PC414_001613"
     CDS             31678..33057
                     /gene="mgtE"
                     /locus_tag="PC414_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HDF2464011.1"
                     /translation="MDRKLDASQDLLYEVKSLIDNNKVLELRELIEEYHIIDIFDIME
                     NLEEDMKIQLFEVLPLDMASSILEEGSVEFFISILSKLDVEHSKNILELMSLGDMADK
                     LSELEEEEREHIINLLNQENADYVKELLFYDEDSAGGTMTTGYISINKDMTALEAIDH
                     MREEAEEAETIYYIYVVDDEEKLVGVLSLRELIIARDANIVEDLMSENIISVYVDEDR
                     EEAVRLVSKYNLIAIPVVDRQEKLKGIITVDDIIDVMEEEATEDMYKFAGSSEHEREV
                     AEKENPTLREQIISALRGRLPWLIITLVGGLLASLILSNLDYIMNPVYAPLVFFIPVV
                     IGMGGNIGTQSSSVTVITLSNKDLNFSNVVREGIVGIITGLLCSIITGIVIYFVMRKL
                     DIVLIVSISLFINMVLGATIGAFMPVLLKKMDADPSTVSSPIISTALDITGIAVYFII
                     TTALLSKIV"
     gene            33130..33285
                     /locus_tag="PC414_001614"
     CDS             33130..33285
                     /locus_tag="PC414_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqzL family protein"
                     /protein_id="HDF2464012.1"
                     /translation="MNNISWEVFKKTGSIDAYLYFCDCKNIHEEIEEVREKKEDDDNP
                     EHPGDST"
     gene            33251..34024
                     /gene="recO"
                     /locus_tag="PC414_001615"
     CDS             33251..34024
                     /gene="recO"
                     /locus_tag="PC414_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDF2464013.1"
                     /translation="MIILNTQGIVLKAIRYKESDIILTLFTRKLGKVSAIAKGAKKNK
                     SSLLSSSQLFSYSNFTLKKQGNMYKVTQSEIIKSFYNISYDIEAFSYATYITKLVENS
                     ILENQTNNRLFILLAQTLYLYTQDNTDNRFITAAFELKFLDYIGFKPIVNKCINCECK
                     ILQNSVFNIYEGGILCSKCSKLFDNNIKLDLTTISLMEYVLRNDILTCSKAKVSKYIT
                     HELENILDKYLKVYVDNINFKSLHVLQSVKNNKGVDNDE"
     gene            34017..34616
                     /locus_tag="PC414_001616"
     CDS             34017..34616
                     /locus_tag="PC414_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HDF2464014.1"
                     /translation="MSSNITIEMVDSVFERVPGATYAEAKEALVMCNGDVIEAVIYLE
                     NKKNTCNCKAKSAKETMEEVFGKDGENIKFQLKELLRKSSVVRIIVEKDGKTMMNIPL
                     TVGVVGLAFVPLATLVGLSAAVISKYRIKVQNEEDGEIVDLGELNEEKLHVLKNMITN
                     AAKDVKDVVVDKKEDDKDVTADLMKEESEKNKEDEDENK"
     gene            34776..35654
                     /gene="glyQ"
                     /locus_tag="PC414_001617"
     CDS             34776..35654
                     /gene="glyQ"
                     /locus_tag="PC414_001617"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HDF2464015.1"
                     /translation="MNFQEMILALQKYWSKQGCIMMQPYDIEKGAGTMNPNTFLRSLG
                     PEPWQVCYVEPSRRPADGRYGENPNRLYQHHQFQVILKPSPDNIQELYLESLKEIGID
                     PSEHDIRFVEDNWEAATVGAWGLGWEVWLDGMEITQFTYFQQVGNIECELETGEITYG
                     LERLAMYIQEVDSVYDLKWNDKITYGEVFNKAEYENSMYAFELCDADMLFNLFDIYEK
                     EALRLMENGLVIPSYDYVLKCSHAFNTLDARGAIGVSQRASFIGRVRNMAKTVAETFV
                     KQREEMGFPLLKDGDK"
     gene            35654..37720
                     /locus_tag="PC414_001618"
     CDS             35654..37720
                     /locus_tag="PC414_001618"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HDF2464016.1"
                     /translation="MKNYLLFEIGVEEMPSRFVGSTLEQLKNNLSKLFDENRIAFDKI
                     NAYGTPRRLTLVVEGLSDRQNDLEEEIKGPAKKIAVDAENNLTKPALGFIKSKGLSES
                     DIYFKQVGKEEYIFATIKQDGKETSEVLKDILPETIKSVVFPKAMRWGGKNLKFARPI
                     RWIVALLNDKVLEFDLEGIISSNVTKGHRFLGESTIEVDSIEDYFEKLEKNYIVLDQN
                     KRKEMIRKQCIEVANSLGGEVEFDEDLLEEVTYLVEYPTAFYGEFDVDYIKLPKEVVI
                     TPMKQHQRYFPVLKEGKLLPNFIAVRNGDSYRIDNVKAGNEKVLEARLADALFFYRED
                     TKRNLESYIEKLKTVVFQVKLGTIYDKTLRLEKLSADILDSLGLVDEKADTIRAAKLS
                     KADLVTGMVGEFDELQGFMGKEYAKVGGENDVVSEAIFEHYLPRFAGDILPKTNAGVA
                     LSIADKLDSIAGFFAIGIQPTGSQDPYALRRQALGILSILMDRKVAVDIKVLIEHALD
                     NYSELDFNKEEVVEQIMNFFNERVKNLFKDLGIRYDVVDAVLSSDINDVSDMHMRALE
                     LNNWLEKDELVEMLTAFNRVSTLAQKAESDKIDESLLKEDAEIKLYNEFKQVKVKVNE
                     LLKDKKYGIALDEFASLRPVVDNLFDTVMVMDNDEAIKNNRLALLKQVYDTMLGICDL
                     SKIVYK"
     gene            complement(37886..39433)
                     /locus_tag="PC414_001619"
     CDS             complement(37886..39433)
                     /locus_tag="PC414_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HDF2464017.1"
                     /translation="MDNFKKILLNELPNFKEYSSKFLNGEINKMQYKGFSGGYGVYAQ
                     RDKKSFMIRLRVSAGVLSQYQLNKIYEIAIKHNLDKIHLTTRQAIQLHDLSINSIVDI
                     MEEGIKNDIFTRGGGGNYPRNVGLSPLSGVDPSEVFDVTPYAVATDKYFIKNATTYHL
                     PRKLKVSYSNCYTDTAHCSIQDLGFVATLKNDEPYFRVFLGGGLGKQPKVALELDELI
                     KPKDALYCVEGMIKFFMDYGNYENKNRARVRYMVESLGEKLFLEKFKEYYKLEKENGS
                     LELNIEEIDYSKPGVKIDIQDSRLIPQKQDGLYTVYIHPVGGILLTKDLSTLLKELDN
                     IENPMIRLGMTEGLYILNLNGNEAKRILEISKTISCNSQLEQSISCIGVPICQMGIQN
                     SQKMLHEIIDYFRLQNNKEILNKAPKLYISGCLNSCGVHQVGSIGLCGKKKNVDGIST
                     DAFELFVGGSFEIGKTRLGKSLGDFKASDIPEMLYKISDASSGNFYEWVNSNDNILNK
                     ITDKYKI"
BASE COUNT        15872 a         4093 c         7068 g        12577 t
ORIGIN      
        1 aatattttag aagttacgat tgtatgattt ttaaatgcat aggcatttac tctaggacta
       61 tagactaata taaaaaaaaa ctttgggaat actaaaacca taaatttata aaattaatat
      121 tttatatact gtaaaacagt gaggagggac agtatgcata ctcaactaca aaagactatt
      181 ggtctttcag cagctctttc tactgttgta ggtatggtta taggttcggg agtttttttt
      241 aagccacaag caatatacac tacaactaat ggagctccag gtctaggtat tatagcgtgg
      301 cttttaggtg gttttataac aataactgct gggcttacag ctacagaaat ttcagctgct
      361 ataccaaaaa ctggtggtat gatgatttat atcgaagaaa tatatggaga aaagttaggt
      421 tttttaactg gatggatgca aactgtatta tttttcccag gtacatcagc ggcattagga
      481 gtaatatttg ctcaacaagc atctgaactt ttaggaatga gcccaaataa tatggcaaat
      541 gtacttccta tagcaattgg agttatacta tttttagcgt tattaaatat aataggttca
      601 tccttaggag gaaaagttca gacagtagcc actattggaa agatgatacc tttaatttta
      661 ataatagtct ttggatttat taaaggtcaa agtagtgaag tattaaatcc atttgtagga
      721 gatggtgtaa atgcatcaaa tgcacttgga caagtattaa tagctacttt gtttgcatat
      781 gatggttgga taaatgttgg agctatatca ggagaaatga agtctccaga gaaagattta
      841 ccaagagcaa tagttggtgg tttatcatta gttatggcag tttatattat aataaatata
      901 gcttatcttt gggtagtacc agcaagtgaa ttagcaacag ttacttcccc agctacatta
      961 gtagcaacaa gattgtttgg aaatataggt ggaaaagtaa taactgtggg tatattaata
     1021 tcagtatttg ggacattgaa tggatattta ttaacaggtt caaggatacc ttatacatta
     1081 gctgaaatgg gaactttacc agcatctaaa actcttttaa aagtaaattc tggaggttct
     1141 cctgtaaatt caatactact tataactgtg ttagcctgtg tatatgcatt atctggtcaa
     1201 tttaatcttt tgacagactt aacaatattt tcaatatggg tattttatgt attgactttt
     1261 ataggtgtta tgaggcttag aagacaaaag ccagatttac acagaccata taaagtccct
     1321 ttatatccaa taataccaat tatagctata ttaggtggac tgtttgtaat aataaatcaa
     1381 atattgactt caacagtcat atctttaggc ggaatattta taacactatt aggtttacca
     1441 gtttattatt atatgaaaaa aagataacta cttaatttaa gttagataaa tttgggtaaa
     1501 ggtgaaaatt aacttttatc caaatttttt atactgttat acaatataag tattatatat
     1561 tgcttgatgt gctattttgt ttgatttata atttagtaat tgatgtttta agataattta
     1621 agaaatgagg agtgaaagat gctaggactt tatgtacata ttccattttg tgtgaaaaaa
     1681 tgtaagtatt gtgattttaa ttcttataaa atggacatag attcaaaaaa aagatatata
     1741 gaagacttaa aaatagaaat ggaattatat agtaataaat tatataaaga taataaatat
     1801 aaaaataaag aatgctgtag tctaaataaa aatgacaaaa taacgagtat atttgtaggg
     1861 ggaggtacac ctagtatact aacctctgat gaaataagag aagtatttat aagtataaaa
     1921 gaaatgtttg atatagatga gaatgcagaa ataactatag agtgtaatcc tgggacatta
     1981 acattagaaa aattaaaaac tatgaaagag attggcataa atagacttag tatagggcta
     2041 caagctattc aagaaaagca tttgaacttt ataggaagaa tacatactta tgaagaattt
     2101 gagaaaaact ataaagatgc attaagtgta ggttttaaaa atattaatat agatttaatg
     2161 tacagtcttc caaatcaaac tctatgtgat tggaaggaaa ctcttgaaaa agttgtacac
     2221 ttaaatccaa cacatatatc agcttattcc cttatactag aagaaggaac tgaactatac
     2281 aatatgtatg aaagtaataa atttgaactt atagatgaaa atgtagatat agaaatgtat
     2341 gagtatacaa taaactattt aaagtcaaaa ggatataatc aatatgaaat atcaaactac
     2401 tcaaaagaag gctataattg tgagcataat attttatatt gggaatgtga acattatata
     2461 gggattggag caggagcttc tggctatatt aatgagaaca gatacaataa tgtagaatct
     2521 ttagaagatt atcatttaag tttggtaaaa agagaaaaac caatacaaga aaatgaaatt
     2581 ctttctgaga aagatatgat agaagaaaag atatttatgg ggcttagaat gaataaaggc
     2641 ataaaatttg aagattttaa aaaaaaattt ggaatagatt ttagagagaa gtataataag
     2701 caaatagaaa tgctattagc tagaaagctt ataaatcaga gctttgaagg tatacaatta
     2761 actcaaaaag gaagagaaat atctaatagt gtatttatag aatttatgga ataattttta
     2821 aggtgttgac aaaataaatt tataatggta tataatatat ataatctttt tagcactcga
     2881 acttagtgag tgctaacagc taggaggtat aataatatgg aattaaatga aagaaaatta
     2941 aatatcctca aagctatagt taaagattat atagaaacag ctgaagctat aggttctaga
     3001 acaatatcta aaagacatga tttaggtgtt agtgctgcta ccataagaaa tgaaatggct
     3061 gaccttgaag aattagggta tttaattcag cctcatactt ctgcgggtag agttccatct
     3121 gaaaaaggct ataagttata tgtaaactct cttatgagta aaagcgaatt ggatgataat
     3181 gataagattc ttattgaaca atgtatgaat cataatataa atcatataaa agaattgatt
     3241 catgaaactt ctaagctatt atcgcaacta acaaactata caactgtagc agttactaag
     3301 agtttaataa atcaaagtgt tataaagcac atacagttgg ttgctatgaa tgacaataat
     3361 attttactta tagttgtaac agataaaggg gacttaaaaa aagctaacct tacaactaat
     3421 gtatatttag atcaatctaa gttaaatttg atttctgata atcttacaag gaagctctta
     3481 ggtaaaagta taacagattt ggatgataat ctaattgctt ttattaaata cgaaataagt
     3541 gagtattctg ggcttataga tgagctttta aatgctttga actcaaatat gaaagaagaa
     3601 gatttttctc tatcattaaa tggagctact aacatattta gttatccaga atttaatgat
     3661 gtattaaaag caaagtcatt tttaaatatg ttggaaaaaa aggaaacaat agctgacata
     3721 attaaatcaa agggaataca aaaagacaat ctaaatataa taattggtag tgataatgat
     3781 tgtgaactgg cacaggattg tagtatagtt acagcaactt ataatgtaga tagagacttg
     3841 gttgggcgaa taagctttat aggccctacc agaatggatt atgctagaat ttactctata
     3901 ataaattata tgagtttatt aattaataga aagtaaagaa attttaaatt gcgaggtgta
     3961 aagtcttgga taaaaaaaat gagcaacagg aagtaagaga agaaaatgat acttctatta
     4021 accaagaatc ggaaactcaa gtagagttag aagaggaagt tgttaatgaa gaatgtgaaa
     4081 catcaagtga aaaaactgat gaaaaagaag ttgatgatga aaatgtaaca gatataaatt
     4141 ctaagttagc agaaaaaaaa ttgcaggatg agttggatga attaaatgat aaatatcaaa
     4201 gacttcaagc cgaatatgca aactacagaa gaagaactca acaagagaaa gagacaatag
     4261 gcgtatttgc aaatgaaaaa ataataactg aattgatacc agttatagat agtatggaaa
     4321 gagccttaga tgcatgtgaa gataaggaag atactatgta taagggtata agccttgtac
     4381 ataaacaatt aatagacact ttagtaaaat ttggagtaga agaaatagaa gcagaatcta
     4441 aggaatttga ccctaatcta catctagcag ttatgcaaga aagtgtagat ggagttgaag
     4501 caaatcaaat agtaatggta cttcaaaaag gatataagct aggaactaaa gttgtaagac
     4561 cttcaatggt taaagtttct tgctaagtat aaattaaagg ttaaattatt taacctaata
     4621 taaaatatta aaataagtaa aacttaatta tataattact cgaggaggat tttagtcatg
     4681 ggaaaaataa taggaataga tttaggaaca acaaattcat gtgttgcagt gttagaaggt
     4741 ggagaagcac aaataatagc aaatagtgaa ggtatgagaa ctactccatc agtagtagca
     4801 tttacaaaag atggtgaaag aatagttgga gaacctgcaa aaagacaagc agttacaaat
     4861 gcagataaaa caataacttc tataaaaact catatgggaa ctgattataa agtaaatata
     4921 gatggaaaat catatactcc acaagaaata tcagcaataa tattacaaaa attaaaatca
     4981 gatgcagaaa gttacttagg acaaacagta acagaagcag ttataacagt accagcttac
     5041 tttacagatg ctcaaagaca agcaactaaa gatgctggta gaatagcagg gcttgatgtt
     5101 aagagaataa taaatgagcc aacagcagca gctcttgctt atggtatgga taaattagac
     5161 caagaaaaga aaatattagt atttgactta ggtggaggaa cttttgacgt atctatactt
     5221 gaaataggag atggaacttt tgaagtttta gctacagctg gtaacaatag attaggtgga
     5281 gatgactttg accaaatagt aatagattat ttagcagagg aatttaaaaa agctgaaggt
     5341 gtagatttaa gaaatgataa aatggctctt caaagattaa aagaagcagc agaaaaggct
     5401 aagaaagaat tatcatcaac aatgagttca aacataaact tacctttcat aactgctaca
     5461 gcagaaggac caaaacactt aaatatagat ttatcaagag ctaaatttga agaattaact
     5521 agaggtttag ttgaaaaaac tatggaacca actaagacag ctttacaaga tgcaggactt
     5581 tctacaggtg atatagatga tgtattatta gtaggtggtt ctacaagaat accagcagta
     5641 caagaagctg ttaagaagtt tataggaaaa gaacctcata aaggtataaa cccagatgag
     5701 tgtgttgcag caggtgcttc tatacaagct ggagttttag caggggatgt taaagactta
     5761 ttattactag atgttactcc attatctctt ggtatagaaa ctatgggtaa tgtaatgact
     5821 aagataatag aaagaaatac tacaatacca actaagaagt ctcaaatatt ctcaactgct
     5881 gctgataacc aaacagctgt tgatatacat gttcttcaag gtgaaagaag tatggcttat
     5941 gacaatacaa ctttaggaag attccaatta acagatatac caccagcaca aagaggaata
     6001 cctcaaatag aagttacttt tgatatagat gcaaatggta tagttaatgt ttcagctaaa
     6061 gatttaggta caggaaaaga gcaaaaaata actataacat caaacactaa tttatcagaa
     6121 gcagaaatag agcaaaagat aaaagaagca gaaatgaatg cagaagctga caaacaaaag
     6181 aaagaaaaaa tagaagcatt caatcaagct gaatctacta tatatcaaac tgaaaaaact
     6241 ttaaatgaac ttggagataa aataagttca ggtgaaaaag aggatataga aaaagctata
     6301 gcagacttaa aagctgtaaa agataatcaa gatgctacag cagaagaact taagaaagct
     6361 acagatgaag taatgactaa gttccaaaaa gtatctcaag aaatgtacca aaaagcagct
     6421 caagaacaac aagcggcaca aggtgcagaa caagcacaag acaatggacc taaagatgac
     6481 aatgtagtag atgctgactt taaagaagta gatgaagata aataagctat ataatatgta
     6541 acactaaatg ataattggaa catatatgat ttgtgaaaat catatagatg ttaaagatat
     6601 atggacaata tacatcataa agaatataat atatcataga ttatataact taaagtagaa
     6661 tataagaatt aatgatagct tgtagtttat tattctacaa gctatcgttt tgtaaaatga
     6721 aggtggtgtc aatttttgag tacaaaaagg gattattatg aagtcttagg tatcagtaaa
     6781 ggtgcagaag ctcaagagat aaaaaaagct tatagaaaat tagcaatgaa gtaccatcca
     6841 gatagaaatc ctggagataa agaggcggaa gaaaaattta aagagataaa tgaagcttat
     6901 gaagttctat ctgatgatac aaagagaaaa acttatgacc agtttggaca tgatgggcta
     6961 aatggtcaag gcggttttgg tggtcaagga ggatttggcg gtcaaggctt tggtggtttt
     7021 gaagatatgt tcggagatat atttggtgac atgtttggag gaagcttcgg aggtggaaga
     7081 gcaagaagaa gagggcctca acgtggtgca gatataagac agagtgttac tatatctttt
     7141 gaagaagctg catttggtaa aaagatgtct ataaaagtaa atagaagtga agaatgtgaa
     7201 gaatgtaatg gaactggagc aaaaccagga acatcaaaga aaacttgttc aacttgtaat
     7261 ggaactggtc aagttagaac tgttcaaaga actccatttg gtaatatagc aagttcaaga
     7321 ccatgtagtg cttgtaatgg aactggagaa gtgattgaat ctccatgtag taaatgtcat
     7381 ggaactggaa atacaagaaa agttaagact atagaagtag atatacctgc tggtatagat
     7441 gatggtcaaa tgataaaact ttctggtcaa ggtgaagtag gagaaaaagg tgctccaaga
     7501 ggagacttat atattgtagt taatgttaag tcacatccac tatttactag agatggaaat
     7561 gatatttatt ttgaaatgcc aattacattt gttcaagcaa ctttaggtga tgagatagaa
     7621 gtacctactt tggatggaaa agttaaatat agtgtgccag aggggacaca aactggaact
     7681 gtatttagac ttaaagagaa aggaatccct agaattagag gtaattcaag aggagaccaa
     7741 tatgttaagg ttgtagttga aattcctaaa aaattaaacg ataagcaaaa agaactattg
     7801 agagaatttg cgaaggagtg tgggtcgaac gtccacgaaa agaagaaaac cttcggacaa
     7861 aagatagaag atatgtttaa gaaaaagtag ttaaagtagc caatatataa tcatattttt
     7921 aaaatcttag ttatttataa gattgtcaaa ggagatatta tgatttttat attggctatt
     7981 tttgtataaa aaataaatgt gcataaaata aaactgaata taaataattt tctgaaaaat
     8041 acttgtccta aaatatataa tatgctataa tataatcaaa taaaagaaag gggtgattcc
     8101 agtgggaacg gatgtggcac tgccaaatga tgaagaaaac tttattgtaa tatacaaaat
     8161 aaaattagat acttttagtg ctagattaag tactggaaaa actcatgagc tgggataatt
     8221 attgtaatta cctagactca aacttttagt agtaaaatag taatattata tagacaaggt
     8281 atctaatttt taaatctttt gtatgaatta aaatttttat aaagggtata tctgattaga
     8341 atgtaattag ggaaacctag ttgtgaatta cttttagtat atacatgtgt aaagaaatat
     8401 gtatatggta ttggttattg tgctagatgt gtatgtaaag gaatatgtgt ctagttgggt
     8461 atatgtaaag aaatatactt ataatctatg aaaaattttt agtataaatt gctaaaacat
     8521 tgagattgtt ttttatagaa agttagttta taccaactaa aaatgtgctt aagatttgtt
     8581 tgatgtttaa ttataaataa ggttgtagat aaaaaaataa gtaaagacaa aggtttaaaa
     8641 tacttgcttt aaatatagtt tgttttgaac acttgccaaa ttaaaataat taataaaata
     8701 gacatcttct catagtaaaa tatactgtga gaggatgttt ttgctttgtt cataaacttt
     8761 tatgaaaaat ttgcatagtg tcacatttta atatactata tatataataa aatatagata
     8821 aaattaaatg attcaaaaga gaaaggtgaa aagagatgta ttatataggt gtagacatag
     8881 gaggaacagg tatacaagca ggagtagttg ataactatgg gaaaataatt tttaggtcag
     8941 agtgtaaaac agtaattgaa aaaggctttg aaggaatact taatgacata aaaataatga
     9001 tttataagtt gttggaagat aataaactta ctatgagtga cataaaatct attggatttg
     9061 gtgttccagg ttttataaat aaagaaggat tagtaacttg tgtaaattta aaatggaata
     9121 aaaaagcttt taataaagag ttaaaaagaa gatttccaga tgttgaaatt catggagaaa
     9181 atgatgcaac agtagcagca cttggagaag caaagtttgg aagtatgaaa ggtgctaatg
     9241 ttggagtatt atatacttta ggaactggag taggtggagg aatagtaatt aatcaaaaag
     9301 tcttttctgg agcacatgga ttgggttcag aaataggtca tcaaattata ggagaaaact
     9361 attttaattg taattgtgga aataatggtt gtgtagaaac tttttgttca gcaactgcaa
     9421 ttataaagta ttctcaaaaa ctgatagaag aaggagaaaa gagtaggatt ttagatttgg
     9481 cagagggaaa tttagaaaat gtaaatgcaa agatggtgtt tgatgcatat agagaaaatg
     9541 atttagtggc aattaaagta ataaatagat ttaaagagta tttagcaaaa acttttgcaa
     9601 acacaataaa ttctttagac ccagaaatta tatctatagg aggaggcatt tctaaatcta
     9661 gcgatataat acttgatgga atagaagatt tggtaagaaa atttgtatta tacaaaacag
     9721 aagatatagc tacaattaca tgtgcaactt taggttcaga cgcaggaatt ataggagcag
     9781 cttttttata atgtaaatga actgcttatt ggaggcgtga tgatatatgg gtaaaacaaa
     9841 tgctagaaga acgctttttt tgatagcaat aggttcagga actatgctga atcctcttaa
     9901 ttcttctatg atttcacttg ctttacatag tatacaaaat gattttcata ttagttttac
     9961 aactgtatct tggattgttt cagcatttta cctagctagt gctattgcac aaccagtttc
    10021 aggtaagata ggagatttaa taggtagaaa ggtcttattt ttgagtggtt tgatgcttgt
    10081 aattttatca gcatccatag taccacttgt tcaatcattc tttatattga tttttataag
    10141 agtaattcaa gcaattggaa caagtaccct atatccatct ggagttgctc ttatacaaaa
    10201 taatataaaa gaaagacaat cttctgcatt agcagttcta accatttttg cttcaactac
    10261 agcaggatta ggtccgacac ttggaggatt attgttggac ttaggaggat ggcatgctat
    10321 atttcttgta aatatacctg ttgttttggt ttctatatgc ttagggtatt ttttattccc
    10381 aaaagaagta aaagaaaaga aatctataaa agaaacttta aaaaatgttt tgtcaagatt
    10441 agatattatt ggcatacttc tattttcaat tgcaatgatt tttatattac tatttttgct
    10501 atctatgaag gagtctttta acttggaaca acttgtattt ggtataatac tgatgtgtct
    10561 ttttatttgg catgaattga gaacaaaatt gccatttata gatattaagc tttttgtaag
    10621 taatcctaaa ttgtcaaaag tttacttgca atttattata ttaaatttct ttaattatat
    10681 tttgtttttt ggtcttccta gttattttca agatgcctta cattatagtg caaagtcgac
    10741 tggattattt atgctattta tgtctggtat aggtatattt atatctccat taactggaaa
    10801 atggatagat aaatctggaa ctagatttcc tcttgttaca tcatcaatct ttatgtttat
    10861 tagtgcagta cttctttcag tagtttttat ccatatacct gtaataggaa agggtattat
    10921 attatcatta gcaggtatta gttatggagt tggtaatgta gccctgcaat cctctatgtt
    10981 tgaagaaagt cctcgtgaca gtatagggac agcttctggt ctgtttcaaa ctagtcgtta
    11041 tttaggttct attttagcaa ctgttttact tggaatggta tttgaagtaa atataacatc
    11101 agaacaattt cagatattag gttatgttat ggtggtctta ggtacaataa gttttttatt
    11161 aaattttata ttaaagaaag aattaagaaa agtagaatag tggatttatg gatttaataa
    11221 gtctaataac tgataagttt aaaaatattg tttgtaaaac ataactggtt tgatatttat
    11281 taattctaag ttttgaagca tagagtactc gataagtata caatattttg aattaaaatt
    11341 aagcagaaat ttgaactagt ctttcaaatc tctgcttaat ttagtagtta aattttttta
    11401 tagagaaaat aagatttgac acttagataa aaaatcattg cattttcaaa attctttaaa
    11461 tcaaacccaa gtatctcatt aatcttttcc aatctatata ctagcgtgtt tctatgtaaa
    11521 aacatactag tagatgtctt agaaatattt aaatcattat ttataaattt tattcctgta
    11581 tatagaagtt cttcgtcaat agatttaatc ttattaaaat ctattaattt attgagtata
    11641 ttatcctcat taattaaatc taaagtttta tatctaataa agtcaaacaa atttaaaaac
    11701 ttattttcag cgttgtcttt tataatatga ggtagtaaat cagaacataa ctgtaatcta
    11761 aaatcaaaat cagctttact atctaattca ccaagataaa aaatataagt actttgataa
    11821 gtttcagttt caattatatt ttgtgtagtt acattaatat ctaagttata tttagaattt
    11881 actataaaaa aaccattgtg ttgtattata tttatatctt caaataaatt ataaaaaata
    11941 ttttttagat attcaaagtc tatatgagat tggtaaatta aataaaagtt gccttttaaa
    12001 ttatcgagat tatttttatc tagaaaatca ataacataaa tagcattatt gaaagtaact
    12061 aaatggctac tagcatctaa cttttggttt tcatatggat agatatttat ttttttatta
    12121 agctgtcttt ctaaaaaatc ttttaactct tccataaagt ctccttaaaa ataagattat
    12181 attcttatct tatagctaaa gtagtttctt tgtcaaatat gtgtactcta ctattgtcaa
    12241 ctgaaagtgt taagtcatca ccaacattaa ttctgttttt actgtcaaat ctaattgtta
    12301 tcgtattttc gcctagtttt aagtgagcat aagtttctgc tcccataagt tcttttattt
    12361 caattcttga tttaaaacaa gtatctggat tagatttaat aaaagtatct tctacatgaa
    12421 tatcttctgg tctgatacct ataacaactt cattattaat ataattgtct tttattaaat
    12481 catagtgttt ttctttattc aatttaagct taatatgttc attttgtgca taaatttcat
    12541 cattttcttc tattaatatt acatcaataa aattcatctg tggtgctcca ataaatcctg
    12601 caacaaatat attagctgga taatcgtata catcttgtgg agttgcaatc tgctgaacta
    12661 ctccatcctt cattactacg attctatcag ccatagtcat agcttcaact tggtcgtgtg
    12721 ttacatagat aaatgtagtt ccaagctgtt ggtgtagctt agttatctct gttctcatag
    12781 cagttctaag ttttgcatct aagttggata gaggttcatc cattaagaat accttaggat
    12841 ttctaactat tgcacgtcct aaggcaaccc tttgtctttg tcctccagat aatgttttag
    12901 gttttttatt taaaagaggt agtaagtcta atattttagc agcttcttta acttttttgt
    12961 ctatttcatc tttaggcaaa tttctaagtt taagtgcaaa tgccatgttt tcgtaaacag
    13021 ataagtgggg atataaagca tagttttgaa aaaccatagc tatatctcta tcttttggtt
    13081 ctatattatt gacaagttta tctccaatgt atagttctcc ttcagagatt tcttcaagac
    13141 cagcaatcat tctaagtgta gtcgatttac cacaaccaga tggtcctact aaaacgataa
    13201 attctttatc tttaatgtct atatttatat tttttactgc gttaaatcca tttgagtaaa
    13261 gttttgatat attttttaaa attacttctg acataatatc ctccttgata tttaaatttt
    13321 aattatatag tatcaaattt ttttattata ttaaatgtgt aaaatgacta aatgtatttg
    13381 tttaatatgt atatttagtt tttagcaaag aataaaacta acagtagaat atatttaaaa
    13441 aattaaaaaa caaaattaaa tttattatca aaaaatcatt ttatgatatg ttttattttt
    13501 atattacaaa tagagaataa tgaattatac tagaaagtag gaggtgaaaa tatgagcaga
    13561 atttcaattt ttatggaatc atgtaatggc aaatttccaa atgaaaattc aatgttgtca
    13621 gtttccttta taaaaaatat tttaaatgca gaaaacaaag attttgctaa aaacatattt
    13681 aactatgggg aagaaagaaa aaacaataag caaataaagg atataaatac agccatttac
    13741 ataccaaatc ttataagaag caaaaatgag ttacttgttg atggagatat aatattcaat
    13801 ataagttttt ataattattc tatgttcagt aaattataca atggaatttt aaaagtaaag
    13861 gagctagaat ataaaggtta tagatttaaa ataaaaaata taaaaataca taaagaacac
    13921 gagataaaag aaaatggtgt tatatttaaa actatgtctc ctataatagt aaagaataaa
    13981 gaaggtaaat atttagatgt agaagattca aattatatag aatgtttaaa ctatatagct
    14041 aatctaactc tggagagtat aagaggtagt gggcttagaa agccattgga atttatacct
    14101 attaacttca aaaaaagagt gttaaaagaa aagattcgtg gatttaaagt gagagaatat
    14161 tactatataa atgcttatgc aggaacattt tttttaaaag gagatatgga agatttaaat
    14221 gcactgtata aaatgggaat tggatataga aggacagaaa atgcaggaat ggttgatata
    14281 ttaaagtagc gaggtgagag aaataaaagc taaattttat agatcaggtg atattgtaag
    14341 agatattggc attgtttact tttatgaatt actaatggat ttaaaaaacg aactagaaaa
    14401 aaatgattat aagctaaaat ttacttgtga gttaaataga aattatttat ctttagaatc
    14461 tgcagaatat atagacccta agtatatatc tgattatata ttagaaaatc gactttttaa
    14521 ggtatttaaa aatgggaaaa aggaaacctt agaaaaaata tttagtaata tagataattt
    14581 aacgtatgta aattttattg aagaattaga taaatcttct gcaactgcta aacaaaagga
    14641 agatattaaa aaagatttta aaaacaggag gtttccatat gttagaaata gtggtaagta
    14701 tggattaaac acgagtttag aaaatatgga aacaaatgta aaaagaatag tagatactgt
    14761 aattaaatca aatatagaga tagatgaaat agataaactt gatttgacac aatatgaaga
    14821 aaaagatagt gtatgtaatg tttgcaatat aaataaaaca acaaaactag atattgattt
    14881 aagagaacga aaggactcta agtataactt tttatttaga ggtgcagaaa aatcaggctt
    14941 taaaagaagt ggacaagttg aaagtaatat ttgttttgag tgtgaatttt tcaacttaat
    15001 gtgtctttta tatataaatt taaaaagacc aatggttttt gcatatacga atgatttaag
    15061 agaattagca tttttaaatc ataagataat gcttaaaaga aaaatgtatt cagataaatc
    15121 attttataaa aaacttttgc atgaaaaaat aagttcaatt agactatata gctttgatat
    15181 tgatacgaat aaaggtataa ttctaaagtt tgattctatt atagagtata aagaattatt
    15241 aaaaaaaatt gaacttatag atattataga taattataat ttttcaagag agccaggtaa
    15301 tactagaaat ttgggtaaaa gaatgattga taatgggaat ttatcaaatt taaaagaatt
    15361 attacttgat aatataagtg ttttaaagca atcaactgga acttctcgag aaatggattt
    15421 tgtatctagc tcatataata tcggtttata tataaaattg tgttggattg tagatggagg
    15481 ggaggagtat aaaatgaaaa actatatgga aagtaattta atatattcaa gagtaggtaa
    15541 agacttattt aataaaatga cagatgaatc cagaagaaaa aatttttcta tgaaagtgat
    15601 tcaactactt aagtctaatg atagaactca gctatttcaa actataatgc atgtgttagt
    15661 ttctaatgga atcttaatag gagaagggtt tgtagaaggt ctaatgcaga gttctgaatt
    15721 agaattaaat acgaatgtag gtctatttat tcaagaaatc atgaaataaa aaagggggaa
    15781 aatttaaaat gaatgaaaaa agagtaaaag gagtaacatt aactatactg acagaaagcc
    15841 cagttccact ttcatatgac caagggtatg gaaattatac accaataaaa aaagaacaat
    15901 atagagaaaa aatacatgca aaaactagta tagccacaat aacatatgat ttaagaagaa
    15961 tgctccacca agaatatggt tggaatttaa gtaatattgt ttttggaaaa aaaggaaata
    16021 tatatccatc tattaagaaa aatgtaggat gtgtagatga aaatggatta gagactgatg
    16081 tatttggtta tttaatacca cttaaagata aaggttctat aagtaaagct tctccactta
    16141 gaataatacc ttttaggtca ttaaatccat ataaaggttc aacacagcta ataacaaata
    16201 gaggcttttt aagtagtgaa tttggtagaa aatattatga tgagaaggaa gaaaatgaag
    16261 tacctagaga tgaaaacttt ccaacatcac aagcattagc aatagaagag actttatcag
    16321 attactacgt ttatacaata acattggagc ttgatagaat aggagtagta gaagtagaag
    16381 atggaaagtt acttttacca gaagaaagaa aatttatgag taaggagtta agagaaaaag
    16441 cagtaaaaga tatattagat gcaattactg tgtttacaag aaacataaaa cattcatcag
    16501 tactattaaa accacttgca gtaataggtg gagcttttga taaagttgta ccattttttt
    16561 gggatgatgt agattataat gctgatagtg gtgagataaa tttagaggga gtcatagaaa
    16621 ctatagaaag ttatagcctt aaagaaagta acacaatact tgctatcaat gatagattaa
    16681 aaatttctaa taaaaaggaa ttaaataagt ttaatttaag taaataccct gtaaaagaga
    16741 ttaaaaatct agcagataga ttggaaattg gagaagataa tatgtggtat ttaaaagagt
    16801 aggaggtttt ttgaattatg aaagtattac aatgtaaact taagcaacca actgctcact
    16861 atagagaccc aaaagtattt caaaatgaat atataagtac tttaaattta ccatctaaga
    16921 ccacaataat gggaatgata acgtatttat gtgatagaag attaaactct gatatagata
    16981 taggaataat tggaacacat catcatagag aactagaatt ttcaagagga gaaaacattg
    17041 atttttggaa tgaatatata aaaatgggaa aaggtaaaga taaagaaaag tttttgctta
    17101 aaggaaacta ctatgattac tataaagagc ataaagctca aaatagcata ttaaactatg
    17161 aagttcttaa agaagtagaa ctgactatat ttatttcttg caaggatgat gaagaacttg
    17221 aatttattag aaaaaagctt gaaagtccat gtaaatatgc aaatttagga agaaaagaag
    17281 attttgtaat accaagtgaa aaagggtgtt ttgtgaaaga agttaagtta aaagaggtta
    17341 tacctatgaa tactcgtgat gctataaaag aaaatataaa attaaagaat acatatgtaa
    17401 gagttgactt aagagataaa gagaatgtcg agactataat aaatcaaggt gtattgattg
    17461 cattgcctca taaatataaa gatttagaag caaatagaga tgatagagtt tatgagtttt
    17521 gccattatat atatgttgat aatgatggta tatatcctaa aaatattaag gtaaatgtat
    17581 gtattgatac aaaagaagtt tttacatggc tttaaattat tttgaaagat aggtgtttat
    17641 catgaaaaat atcatgcaaa aagaaatatt agctaagtca tacaatttaa gaactaatca
    17701 acaagaacaa acattagttg aacatattaa ggatttattt gaagtattag aatctatatt
    17761 agaattaaat ctatattcag ataaagatgt agagatacta aaaatatgtt gtgctttgca
    17821 cgatttagga aaaattaatt caattatgca acaaaaaatg gagattaata ataaaatatc
    17881 gtatagttgc tcagaagaag agaggaaaaa gttagagtct gacaaaaaat ctttaaaaaa
    17941 gatagcaaga cataatatat tttctggagc atttttaaaa gatatattag aaaaaatgaa
    18001 cttgtcagaa gaagataagt tatatatata taaaagtata atgttacatc atggaaacta
    18061 tgaagattat atgaggttaa gtattagtaa agttcaagaa gaaatttatg agtatattga
    18121 aaaaggcatt ttagaaaaag aagagtttaa tctgaaagat atagaaagtt atataaatag
    18181 cattttaaat gtagacttta aatttggtga agatgtattg gattatgatt acatagataa
    18241 attaagtgaa agtatgttta tagacagtga ttataacaat ggtcaaatag atgattcagt
    18301 tttaaattat agaaagttta agtatatttt atataaaggg atgctgaact taatagacca
    18361 tagtgcatca agtagacaga aaggtataaa attttataac gattttactg atgaagaaat
    18421 tgataatatg attttaaatg agatatataa gagtcagggt aatttggaaa aaaataatat
    18481 agagttcaat actatacaaa aaaggttgag agaactttct ggaagaaatg ttctgactgt
    18541 tgcatttaca ggttcaggaa aaacagctgc agattataga aagactttta aaagaaaatt
    18601 ctttttagtg ccaaataaaa tatcagcaga aagtttttat agaaaaaata tttttcaaaa
    18661 taaaaatgat ttagatacaa gaagtagtaa tgattacatt ggattacttc atggagatat
    18721 aaacttatat tcagagaatg aagataatgg agaacatgat tttgtattga cattaaggga
    18781 tatagatttg agtataaact tttgcaaacc ttgtgtaatt gcaacagttg atcagttatt
    18841 acttagtatg tttaaatttc cgggatatga aaaaatattc gcagccgtaa aaaatgcatc
    18901 tataactgtt gatgaagtac atttattgga accaaagatg tttttgattt tgatatattt
    18961 tatgcagttt gcatgcaagt acttagatgt tgattttcac ttaatgacag ctacacttcc
    19021 aaaatcatat aaagagcaaa tgataaacaa gggaattatt tttcaagaag aaagtaatga
    19081 aaatgttact gataaaggag aaatagtttt tattgagagc aacaaggaag aagagatttg
    19141 tgaaggtaaa gatgtaaagg tttcttttat aaaggaaaaa gaaatcaagt caatagttga
    19201 agatgcttta gaaaataaac aaaaaattct tataataaag aatactattg attcagtcaa
    19261 tagaacatat gagtttttaa aagaaaattt atctgataag tatggtggtg tagatataga
    19321 tgttttacat agtagattta agttcaaaga taaaaaagag aaatatagta aaatattaaa
    19381 tggagagggt gatatttgga tatcaactca atcagtagaa atatccttgg atttggattt
    19441 caatataata ataagtgatt tagcaactat ggatagctta atacaaagaa tgggtagatg
    19501 caatagaaat aataagtatg aatatggaaa cttttatatt ctacctagtg aagataaaat
    19561 ttatgacaac aaacttaaga gcacaactaa aaacatctta aaggacatat taaaaactaa
    19621 ctctattttt acaatgggaa ttagaaaaac tattttggat gattactatg ataatagtgt
    19681 agtcaaaaaa tattttgaag agaattttat aagttgtgat aaagaaatta agaatattta
    19741 tggtgtaaat aaagaaattt ttgatggatt ggatttaatt tttaattttg aaccttacaa
    19801 aaatattgtt gatagtaaga aggaagcagc taaaattttt agagatgttg atgttagtta
    19861 caaaattatt ctggaagaag atttttataa ggaagataga gagttacaac aagattcgat
    19921 tcaagtatca gggtttatat ttaatagatt atattattat gggttaataa gcaaagtaga
    19981 aggatacatg gtactaaaat ctagtagtaa gtttgagtat aatagtactg taggcttaat
    20041 tttgaagtga gcatatgttt aaatataata tacctttaga cattgaaaat acaaaaagta
    20101 ctactgtaag ctcactgtaa aaactatttt ttaaaaggaa aaataagttg tgagatagag
    20161 tgtttacaag tgtatataga taataaattt gaggtttaag attaactaag tgggtttcag
    20221 gtacatcgcg aaaataatta taagttggag attttgtatg tttaagatta actaagtgga
    20281 tttcaaatat tgatatagta gatataatag aagttttatt attaggttta aaattaacta
    20341 atcggatttt taaatattct gactattata taaggatatt attagattga atttaaaacc
    20401 aataatataa aacaacaaaa tattatattt atgatataat aaaagttatg gaaaaacagg
    20461 aatattacta tagagaagag aggtaaactt atgaataatt ggatagaagt aactataaaa
    20521 acaacaacag aagcagttga acctataacg aatatactat atgaacaagg agctggagga
    20581 gctgtaatag aagacccaaa agacttttta ttccaaaaga aaaatgaact agactgggat
    20641 tatgttgaag aagaagtatt taaaaagaat gaagaagatg atgttcttat aaaaacttat
    20701 gtttctgaag aaaaaaatgt gatggaattt gtcgaaataa taaagcaaaa agttttagga
    20761 ttaaaagatt ttggtataga tataggtgaa ggaagtgtat cattggacca agtaaatgaa
    20821 gctgattggg caaatgcatg gaaagcatac tacaaaccaa caaaagtagg acaaagggta
    20881 gtagtaaagc ctacatggga agattatgct atgcaagagg gggatttaat catagagctt
    20941 gaccctggaa tggcatttgg tacaggtaca catgagacta ctagtatgtg cattagagaa
    21001 ttagaaaagt atgtgaataa agattctaaa gtatttgata ttggatgtgg tagtggaatc
    21061 cttgcaatag cagcagctaa attaggagca aaagaagttg tagctgtaga tttagatgaa
    21121 gtggctgtaa aagttgcaaa agaaaatgtt cttgaaaata aagttgaaaa aagtgtatct
    21181 gtaatgcatg gtaatttaac tgatgtaata aaagataaag cggatgtaat agttgcaaat
    21241 ataatagctg atataataaa gatacttgca aaagatgttc aaaactttat gaaagaagat
    21301 gctatattca tatcttcagg tataattttg gataaagtag aagaagttaa agaaagctta
    21361 attgaaaatg gatttgaaat agttgaagtt caaaaattag gtgaatggtc agcaatagtt
    21421 tctaagctta aaaaataagg gatatggtga cgtataatgg atagattttt tgttgaaaaa
    21481 aataatataa atctacaaga taaaacttgc actatagaag gtgaagatgt aaaacatatt
    21541 tcaaaagttt taagatgcaa attaggtgag aaacttgaaa tatgtgacaa gaataataat
    21601 gagtatattt gtgagataat gaatatagat aagagtatag ttaatttaga aattttagaa
    21661 aaagttgata taaatagaga atcagaactt aaagtaaggc tataccaggg acttccaaaa
    21721 gctccaaaaa tggaaataat acttcaaaaa ttaactgaag tgggtgtaga agaaataata
    21781 ttagttcaaa ctaagagaag tgttgttaaa gttgatgaca aaaaagaaga taaaaaattt
    21841 gaacgttggg aaagaataat ttatgaagca gcaaagcaaa gcaaaagagg taagatacca
    21901 aaattaagag gtgttttaag ttttaaagaa gctttagaag atatgaaaaa aaatagtgta
    21961 aatatttgcc cttatgaaaa tgaaagaaca gtttcaataa aacatgcact aaaaaaatgt
    22021 gattctaata tagatagtgt tggaattttt ataggtccag aaggtggatt ttctgaagaa
    22081 gaaatagaac aaatacaaaa aaataattgt aatgttgtat cattggggcc tagaatatta
    22141 agaactgaga cagcttctgt tgtagcatca acaatagctt tgtatgaact aagtgattta
    22201 ggaggagaaa aataaattga aaaaagtagc tttttataca cttgggtgta aagttaatca
    22261 atatgaaaca gaagctatgc tagaactatt tgagaaagat ggttatgaac aagtcaatag
    22321 tgaagagtat gctgatgttt atgttataaa tacatgtaca gttactcata tgagtgatag
    22381 aaaatctcgt caatatataa gacgagttaa aaagaagaat ccagatgcaa taatagccgt
    22441 tgtaggatgt tattctcagg tatctccgga agaaattttg gatatagaag aagtaaattt
    22501 agtcatgggt acaaatgata gaagaaagat tgttgaagaa attaaaaaga taaattcaag
    22561 taagaaagtt agtacagttg atgacataat gaaagttaaa gcttttgaag aaatagaaat
    22621 cagccaaact aatggaaaga ctagagcatt tatgaaaata caagatggat gtgacagatt
    22681 ttgtacttat tgtatcattc cttatgcccg aggacgcgtt agaagtagag atattgatag
    22741 tatagtcgat gaagttaaaa aattggctaa taatggctat aaagaggttg tattaacagg
    22801 aatacatgtt gcctcttatg gaaaagattt aaaggatagg gatattaaac ttcttgatgt
    22861 aataaaacaa ataaaccaga tagaaaaaat agaaagaatt aggcttagtt ctgttgaacc
    22921 aattttattt acagatgaat ttgtaaatga agttttaaag atggataaag tttgtcctca
    22981 ttaccattta tcacttcaaa gtggatgtga tgaaactcta aaaagaatga atagacgata
    23041 cacaacttta gagtacaaaa ctattgtaga tagattgaga agtaaaatgc cagatgtggc
    23101 aataactaca gatgtaatag taggttttcc tggagagaca aatgaagagt ttaaaaaaac
    23161 gtacgagttt ttaaaagaaa tagagctttc acaaatgcat atttttaaat attcaccaag
    23221 aaaaggaact ccagctgcaa ctatggaaaa tcaagttgac cctcaaatga aacactttag
    23281 aagtgaacaa cttttaaatt taagtaaagt aaattttaat aaatttgcaa ctaaatttat
    23341 cggccgtgag ttggatgttt tatttgaaca aaatatagaa ggcaataaat atgagggatt
    23401 aacttcaaat tatataagag ttgttgttga aagtgacaaa aatatacaag gtcagatatt
    23461 aaaagtgaaa ataaatgatg taaaagatga atatgtagaa ggaattttgc tataacatgt
    23521 agaaattata atatttataa gataggaggt ggaaagaatg gattgtattt tttgtaaaat
    23581 agcgaacgga gaaatacctt caactaaagt ttatgaagat gatagagtct tagcttttaa
    23641 tgacttgaac ccagttgctc catatcatat attagttgta cctaaaaaac attatgatag
    23701 tttaattgat attcctgata aagagatgga cattgtgtca catattcatg ttgttattaa
    23761 taaaatagct aaagagaaag gttttgacca aactggattc agagtcataa ataattgtgg
    23821 aagtgatgga gggcaagaag ttaaacattt gcactatcat attttagctg gtaaaaagct
    23881 tcctaattac gaagcgggac aaaactaaac taaattattg aaaaattgag ttccttcttg
    23941 ataaaaatac ttgataaaag taaaaattta gtttataata aacaaagtgt taggttgtaa
    24001 tgcatattat gcaaaaagaa catagttaca gtcccagcat aattgcgcta ttggagggag
    24061 ggaaaaagaa atgtcagaag taagagttag agagaatgaa acattagaca gcgctttaag
    24121 aagattcaag cgtcaatgtg caatgtctgg catcatgtca gaagtgagaa aaagagagca
    24181 ctatgataag ccaagcgtta agcgtaagaa aaaagctgaa gcagcaagaa gaaaaaacgc
    24241 taagaaatag tagtaaataa gaataaagag gtgaagggga tgtcccttaa gcaaaagtta
    24301 caagaggatt tgaaatcttc gatgaaaaac aaagatacag tgagaaaatc tgtagtaact
    24361 ttaataagag cttctataaa gcagtatgag gttgataata gggtcgagct tgatgaagat
    24421 ggaattatcg atgtaatagc aaaacaatta aagcaacgta gagatgcatt agttgaattt
    24481 gaaaaagctg gaagagaaga cttaataaaa gaaacagaag gtgaaattga agttttaaaa
    24541 gagtacctac ctcaacaatt aagcgaagaa gagttagaag aaattgtaaa atctactata
    24601 tccgaagtag gggcaacttc tatgaaggat atgggaaaaa taatgtcagt tatccaacca
    24661 aaagttaaag gaagagctga cggaaaactt ataaataagt tggtaaaaca aaacttacaa
    24721 tagattgata tagcctagag agaaatctct aggcttttta tagtttttgg gaataacgag
    24781 tcatatttga gcatatctat aatcaaagaa aattttgaaa gaaggggaaa aatttgatta
    24841 tatcagtaaa agagattagg aattttttga taaacatatt tataccacta ataataggtt
    24901 acttaagtag catcttaaca atgattatca gtggaacaga tatttcaacc tattatttac
    24961 aactcacaaa gccaagcttt gcaccaccag cttttatctt tcctatagtt tggactatat
    25021 tgtacatatt gatgggtata tcttcatacc taatattaag aaagggttac aacttaccta
    25081 aagtaagaga tgcaattttt tattatggat tacaacttgt attaaacttt atttggagta
    25141 tactattttt tggatttgga cttagattta cagctttgat agatataata ataatgattt
    25201 tggtttctat aataatgatt tcgaagtttt ctaaaataga taagagatct ggatacataa
    25261 atttgattta tttaatgtgg cttgtttatg caggattttt gaactatttt atatggttta
    25321 ttaataaata gaatgtaaag attttgtaaa ttaccctgta cttttaataa tatcataaca
    25381 aacgaaaaat aaatagacta ttaaaaataa tcattttcat atacaataat attaatttaa
    25441 atacaataaa tttataaaaa ttgtatttaa agattaacat attaaagtat tagaattgga
    25501 aaggcttgaa gggagaggtt tttatgcaaa taatagtatc tgggagacaa atgaaattaa
    25561 ctgacggaat aaaaggatac gtagatggaa aattaagtag gttagaaaaa taccttgatc
    25621 ctgaatcaga agtgaaagta acagtaagtg cgaaaaagga tagacaaaaa gtggaggtta
    25681 caataattcc tattaacggt caaataataa gagcagaaga tgtagaagat gacttatatg
    25741 cagcaattga tattgtttgt gacaaattaa gtagacaagt tgtcaaatat aaaactaaag
    25801 ttaaagacaa agttcaaaat aataagagca taagatttga aaatcttgac tttatagata
    25861 atagcagtga atttgatgat tatgattatg atgatgaaga agatgaaaat atagtaatag
    25921 aaagaagaaa gaaatttaat gtaaaaccta tgagttctga ggaggcgata cttcaaatgg
    25981 aactagtagg acacaatttc tatatgttta gaaaccaaga taattttgaa ataaacatag
    26041 tgtataagcg taaagctggt ggatatggtt taatagaaca agattaataa aaattgtata
    26101 cgtttagatt ttatataaga aaaaataaaa aggtaatcat gttgattttt tatcaatatg
    26161 attacctttt tcttgtttaa ataatatatt agatattagg tatttattat ataaaaattt
    26221 taaaactaat ctgttacaac atcaataaag tgcataaata cctatttcaa gcataaaatt
    26281 aattaagggg gtaatgctta tgttagaaat atccactgat ttacaagtaa atcaaccagt
    26341 tattactgtt acaagtaata cgtttattag tatagaaaat tatctatcaa tattagaata
    26401 tgaagttgat ttaataagaa taaagactaa agttaaaaca attaaaatat ctggggataa
    26461 actttcactt aagtatatta ctgatagtga gataggtata aaagggatta tctacaatgt
    26521 tgagtatgtt gattaggagg agatacactt gataagtttt ataaggggat actatgttat
    26581 agttgtggaa ggagtaggac tcgaacaatt tttaaatcat cttataagaa atgggataag
    26641 tgtttacaat gtaactagaa taaaaaatac taaaatggag tttcatatag atagacaaga
    26701 tataaaagac tttaagaatg tttatagagg aagtaagttt gatataaagg taaagcaaaa
    26761 aactggggtc ccttttatta tcaaaagagt ttataagcac aaaggtatgt ggatttgtgc
    26821 tcttgtttct ttatttttat tgatgtctac atcacagttt gtgacagatg tatatataca
    26881 atctcctgaa ggtataaaaa aagaagctct tagaaatgag ctttataagg ttggagtaag
    26941 gccaggagtc tataagaaga gtattgatag aaaagaagtt agagaccata tgatgtcaaa
    27001 atttaatgat gtggcatatt tatctatcaa tgtaaaagga acaaatatat ttgttactgt
    27061 tacaaaaaag gcagaatccc taaaatctac agaccaatca aattattgta atgtaattgc
    27121 tttaaaaaat ggtataatag aaaaggtaat ccctagaagt ggtaaatcag ttattaaatc
    27181 tggagacata gtccaaaagg gagatgtctt attaaatgga gctaatacca agtcaatacc
    27241 agaagtatgg gcaagtactt tttatgagtc cacaaaaaag gcaagttatg tagatactgt
    27301 aaataaaaaa actggagaaa agaaaaatat atatacacta agtttttatg ataaagagtt
    27361 tacaatgaga aaaaatataa aatataaaga ttatgttgtt gaaaataaag aaaaaaaact
    27421 atcaatagga aactatactt ttccaataaa gataaaaacg agtacttttt atgaaacaaa
    27481 aaaggttgaa gtaaaaagga ataaagaaga gttaaaaaaa gaattatctg aaaaagcttt
    27541 aaaagagtta gaatacataa tcccagcttc ggctagaata atagatgtaa aacataactt
    27601 taaggttaat aaaaatatgt tagaatattt aataacagtt caaactagtg aaaatattgc
    27661 taaaatttat cctcttagta agtcagaagc agaaaggttt ataaaggaag aaagtaaacc
    27721 agatgaaggt gaagaggaag ttccttccaa tccagaaaaa agaccattaa atgatataag
    27781 aaatgagttt gatgaggata ataaggataa taaagaccaa aacaattctg atgaaaataa
    27841 tagtaatcaa aatagtaata atagtcaaaa taataatagc aataacaact aataaaatta
    27901 aacttaaaat cttaggagga ctaattcaat tgataataca aaagaaattt acaatagcag
    27961 atggtaactt tgaaagagag ttatttggca actttgatga aaatgtaaaa ttaatagaga
    28021 agactttaaa tatagatgta atacttagag aaggcaatat aattttaatt ggagaagaaa
    28081 aaaatgtaga ttctgcatta aaacttatga atgaattaca ccaaactgta tcaaatggta
    28141 agcatttaga taagcagagt atttcttatt cattatctct tttgcttgaa ggaagtgaac
    28201 aaaaaataaa agaacttgag gggacaatcg tcataactca aaggggaaaa gctgttcaac
    28261 ctaaaacttt aggtcaaaaa gaatatataa aacttataga aaataatgat ataacttttg
    28321 gtgttggacc tgctggaaca ggaaagactt atcttgcggt tgctatggca gtaaaagctt
    28381 ttaaaagaga tgaagttagt agaattatac ttacaagacc agctgtggaa gcaggagaga
    28441 gtctaggttt tttaccagga gatttaaagg acaaagttga cccatattta agaccactat
    28501 atgatgcctt atttgaaatg ttgggtgctg ataaatttaa taagtattta gaaagaggta
    28561 ctattgaggt tgcaccactt gcatttatga gaggtagaac acttgataat tcatttataa
    28621 tattggatga agctcaaaat actacttctg aacaaatgaa aatgttttta actagacttg
    28681 gatttggctc taaagctgta gttacaggag atgtaactca aacagattta ccacaaaata
    28741 agaagagtgg tctaatacaa gctacagaga ttcttaaagg tgttccaggt attgggtcta
    28801 taatgctaac agatagagat gttgtaagac atgagttggt acagagaata ataagagctt
    28861 atgagaagca cgacaaaaga gaagaattta aaaaagaaga acgtaaaaaa gcgaaaatgc
    28921 aggaaaagac tttcaaaaga aagtaaaagt gtagtaatat agatatatag cattattaat
    28981 gaataaaaag taggtgtagt gaatggattt gatactagat gatagacaag ataaattaga
    29041 agtaagtgag gagttaattg aaaagataaa ggatataatc atagaatgtt tagattatga
    29101 aggatatgat gataattatg aagtgagtct ttcttttgta gataataaag agattcatga
    29161 attaaataga gaatatagag gagttgatag agtgactgat gttttatcat ttcctctttt
    29221 aagtgatgat tttgaagatg ttgagttaga agaagaatcc ctaggtgaca tagtagtatc
    29281 tcttgaaaga gccttagaac agagtataga gtataaccat agttttgaaa gagaagtatg
    29341 ttttttaata tgtcatagta tgtttcatct tttaggttat gaccacgata cagatgaaaa
    29401 tacaaaagaa atgagagaaa aagaagaaca catattaaat aagttaaaca taacaaggga
    29461 gtagttttta tgaaaccaga aaaaactcgt caaggtataa ttaaagcatt taatgcagct
    29521 atagaaggga tattgtatac atttaaattt gaaagaaata tgaaaataca ttatttaggt
    29581 tcagttgctg ttcttataat aagtttgttt tttaactttt caaaattaga aatgataatg
    29641 ttacttatgt ccatatgttt agttgtagtt gctgaaatgt ttaatacagc tatagaaaaa
    29701 gctgttgatt tagtaactga tgaatatcat gttcttgcta agatagcaaa agatgttgca
    29761 gctggaggcg ttctagtggc agcactaaat tcagtagtag ttggatatat tttgttttat
    29821 gataaattga cagatatatc tggaatatta atttataaaa taagagaatc agaattacat
    29881 ataaccttga tatgtatatt attggttctt atagctgttg tagttgtaaa agcattgact
    29941 tctacaggta ctcctctaaa aggaggaatg ccaagtggtc atgctgcctt agcatttgct
    30001 atagcaactg caattacact tatgactgaa agagttgttg cctctactct tgcatatata
    30061 atggctgtat tagttgccca aagtagaatt gaaggaaaaa tacatacatt ttgggagact
    30121 attgctggag ctttgttagg tgtgttgata gctatattag tatttcagct tggaatgttc
    30181 tataattaaa ataaatgtac ataaaatatt taaaataaaa caaatctgtg ctaattaaat
    30241 ttttatgata taatgtagag tattaaataa aaataacttt taattattgt atacaggagg
    30301 agttgttgtg gataacatag aattgttaag gttggcagaa gatgccagac aacattctta
    30361 tgctccttac tcaggtttta gagtaggtgc agctctttta actaagagtg gtaaagttta
    30421 tactggatgt aatatagaat gtgcatcatt aggtggaaca aattgtgcag aaagaacagc
    30481 tatttttaaa gcaatatcag agggtgataa ggatatctat aaaatagcta tagcaagtga
    30541 taactcagaa aataatgagc aaacatatcc atgtggaata tgcaggcagg taattataga
    30601 gtttggttct gatataaaaa taataacggg atatactaaa ggagaggtgt ttgagcatag
    30661 tataaaggat ttgttgccaa actatttctc tggaaatgat ttaaaataaa tttaaaagta
    30721 taaataaatt taagtaatta aaacaagaag ataaaaagtt aggagacagt taatgttcaa
    30781 atcaggtttt gtcagtatag tgggtagacc aaatgtagga aaatctactt taatgaataa
    30841 tgtagttgga gaaaaaatag ctataatgag tgataagcct caaactacaa gaaatacaat
    30901 acaagcagta tatacagatg aagaaatgca gatagtattc ttagatacac caggtattca
    30961 caaaccaaaa aataaattag gtgaatttat ggtaaaggca gcaacagaag cttttaaaaa
    31021 tgtagattta atattatttg tagttgatga ttctaaaaaa ataggtcctg gagatagaaa
    31081 gataatagag gatttaagaa gtgttaagac accaataatt ttagttgtaa acaaaataga
    31141 ccaactagac caaaaagatg agctatttga tataatcaaa atgtatgata gagaaggtat
    31201 ttttaaagaa atagtaccaa tatcagcatt aaaaggaaag aatacggata ccttaataaa
    31261 agttatacaa aattatttag aagaagggcc aaagtatttt ccagattata tgattacaga
    31321 ccagccagaa agagtattaa tagcagaact tataagagaa aaagttcttc actatcttaa
    31381 tgatgaaata cctcatggtg tagctgttga aattgaaaaa atgaaagcga gaaatgataa
    31441 ggaaatagta gatgtatctg ctgtaatata ctgtgaaaga gattcacata agggaataat
    31501 aataggaaaa aatggtagga aactaaaagg aataggtaag tcagcaagac aagatataga
    31561 attgctttta ggttcacaaa taaatcttca attatgggtt aaagtcaagg aaaattggag
    31621 aaatttacaa aattatataa ataattttgg atataatgat aaataaaggt gggttatatg
    31681 gataggaaac tggatgcttc ccaagattta ttatatgaag taaaatcatt aattgataat
    31741 aataaagtat tggagttaag agaattaata gaggaatatc atataattga tatattcgat
    31801 ataatggaaa atttagagga agacatgaaa attcaattat ttgaagtact tcctttagat
    31861 atggcatctt ctatattaga agaaggtagt gtagagtttt ttattagtat tttatctaaa
    31921 ctggatgtag aacactcgaa aaatatactt gaacttatgt ctcttggaga tatggcagac
    31981 aagttgagtg aacttgaaga agaagaaaga gaacacataa taaatctttt gaatcaagag
    32041 aatgccgatt atgtaaaaga gttactattc tatgatgaag attctgctgg tggaacaatg
    32101 accactggat atatttctat aaataaagat atgacagctc ttgaggctat agaccatatg
    32161 agagaagaag ctgaagaagc tgaaactatt tattatatat atgtggttga tgatgaagaa
    32221 aaactagttg gagtattgtc attaagagaa ttaataatag ctagagacgc aaatatagta
    32281 gaagatttga tgagtgaaaa tataatctct gtttatgtag atgaagatag agaagaagca
    32341 gtaagattag tatctaaata caatttaata gcaattccag tagtagacag acaagaaaaa
    32401 ttaaaaggaa taataacagt agatgatata attgatgtaa tggaagaaga agcaactgaa
    32461 gatatgtata aatttgcagg aagttcagaa catgaaagag aagttgcgga aaaagaaaac
    32521 cctacattga gagagcaaat aatatcagca cttagaggac gattaccttg gttaattatt
    32581 accttagttg gagggttgtt ggcttcgtta atactttcta acttagacta tataatgaat
    32641 cctgtatatg ctccattagt attttttata cctgtagtaa ttggtatggg tggaaatatt
    32701 ggaactcaat catcatctgt tacagtaata acattgtcta ataaggattt aaattttagc
    32761 aatgtggtta gagaaggtat tgtaggtatt attacaggtc ttttgtgtag tataataaca
    32821 ggtattgtaa tttactttgt tatgagaaag ttagacattg tattaattgt ttcaatatca
    32881 ttatttataa atatggtttt aggtgcaaca ataggtgcgt ttatgcctgt tttattaaaa
    32941 aaaatggatg cagacccatc tacagtttca tcaccaatta tatctacagc tcttgatata
    33001 actggtatag cagtttactt tataataaca acggcattat tgtcaaaaat tgtttaataa
    33061 ctttatgtat gatttatcct cttcttacta aaaatataag tataaatgta agaagggggt
    33121 tttttaaaaa tgaacaatat cagttgggaa gtttttaaaa aaacaggtag tatagatgca
    33181 tatttatatt tctgtgattg caaaaacata catgaagaaa ttgaagaagt aagggagaaa
    33241 aaagaagacg atgataatcc tgaacaccca ggggatagta cttaaagcta taaggtataa
    33301 ggaaagtgat ataatactta cactatttac aagaaagctt ggaaaagtat ctgcaatagc
    33361 aaaaggagct aaaaaaaata agagctcttt gctttcatca tcacagttat tttcttatag
    33421 caattttacc ttaaaaaaac aaggtaatat gtacaaggtt actcaaagtg aaataataaa
    33481 aagtttttat aatatatcct atgatataga ggcattttct tatgctacat atattacaaa
    33541 attagttgaa aattctatac ttgaaaatca gactaataat aggctattta tattacttgc
    33601 gcagactctg tatttgtata cacaggataa tacagacaat aggtttataa cagcagcatt
    33661 cgagttaaaa tttttagact atataggatt taaacctata gtaaataaat gcattaattg
    33721 tgaatgtaaa attttacaaa attctgtgtt taatatctac gaaggtggta tactatgtag
    33781 taagtgtagt aaactttttg ataataatat aaaattagac ttaacaacta taagtcttat
    33841 ggaatatgta ttaagaaatg atattttaac atgtagtaaa gctaaagtat caaaatatat
    33901 aacacatgaa cttgagaata ttctagacaa gtatttgaaa gtttatgttg ataatattaa
    33961 ttttaaatca ttacacgtat tgcaaagtgt aaaaaataat aagggagtgg ataatgatga
    34021 gtagtaatat aactattgaa atggttgatt cagtctttga aagagttcca ggagcaactt
    34081 atgcagaagc aaaagaagca ttagtaatgt gcaatggaga tgtaattgag gcagtaatat
    34141 atttagaaaa taaaaaaaat acttgtaatt gtaaagcaaa atctgctaaa gaaactatgg
    34201 aagaagtatt tggcaaagat ggagaaaata taaagtttca attaaaagag cttcttagaa
    34261 aaagtagtgt agtgcgaata atagttgaaa aagatggaaa aactatgatg aacattcctt
    34321 taacagttgg agttgttgga ttagcttttg taccattagc tactttagtt ggattatcag
    34381 ctgctgttat aagtaaatat cgtataaaag ttcaaaatga agaagatgga gaaattgtag
    34441 atttaggtga attgaatgaa gaaaaacttc acgttttgaa gaatatgata actaatgcag
    34501 caaaagatgt gaaggatgta gtagttgaca aaaaagaaga tgacaaagat gtaactgctg
    34561 accttatgaa agaagaatct gaaaagaata aagaagacga agacgaaaat aaatagatat
    34621 aaagtgactt ttaaaaaatg aagagaagat aattataaga attatcacta acattgacaa
    34681 attttataga atttgatatt ctaaaagttg tagaagaact attaaaatag cctttcgtgt
    34741 agacgagagg ctttattgtt ataggaggga tttttatgaa ttttcaagag atgatactgg
    34801 cgttgcaaaa atactggtca aagcaaggtt gtatcatgat gcagccttat gatattgaaa
    34861 aaggtgcagg aactatgaat cctaatactt ttttaagatc attaggacct gagccttggc
    34921 aagtatgcta tgtagagcct tcaagaagac cagcagatgg aagatatggt gaaaacccaa
    34981 acagactata tcaacatcat caattccaag ttatactaaa accttctcca gataatatac
    35041 aagaattata tttggaaagt ttaaaagaga ttggaattga cccaagtgag catgacataa
    35101 gatttgttga agataactgg gaagctgcta cagttggtgc ttggggactt ggatgggaag
    35161 tttggctaga tggtatggaa ataactcaat ttacgtattt tcaacaagta ggtaatattg
    35221 aatgtgagct tgagacagga gaaattacat atggattaga aagattagct atgtacattc
    35281 aagaagttga tagtgtttat gatttaaaat ggaatgataa aataacttat ggtgaagtat
    35341 ttaataaagc tgagtatgaa aactcaatgt acgcatttga attatgtgat gcagatatgt
    35401 tgtttaatct gtttgatata tatgaaaaag aagcattaag acttatggaa aatggccttg
    35461 taataccttc atatgactat gtgttaaaat gctctcatgc atttaataca ttggatgcaa
    35521 gaggtgcaat aggagttagt caaagagctt catttatagg tagagttaga aatatggcaa
    35581 agactgttgc agaaactttt gtaaaacaaa gagaagaaat gggattccct cttttaaagg
    35641 acggtgacaa ataatgaaaa attatctttt atttgaaatc ggtgttgaag aaatgccatc
    35701 aagatttgta ggcagtacac tagaacaatt aaaaaacaat cttagtaaat tatttgatga
    35761 aaatagaata gcttttgata aaataaatgc gtatgggaca ccaagaagac ttactttagt
    35821 agtcgaagga ttgagtgata gacaaaatga tttagaagag gaaataaagg gtcctgctaa
    35881 gaaaattgct gttgatgcag aaaataattt aacaaaacca gcattaggat ttataaaaag
    35941 caaaggatta agcgaatctg acatatattt taaacaagtt ggaaaagaag aatatatatt
    36001 tgcgactata aagcaagatg gaaaagaaac ttcagaagta ctaaaagaca tattaccaga
    36061 gactataaaa tctgtagtat tcccaaaagc tatgcgttgg ggaggaaaaa atcttaaatt
    36121 tgcaagacct ataagatgga tagttgcatt gttaaatgat aaagtattag agtttgattt
    36181 agaaggaata atatcttcta atgtaacaaa aggtcataga tttttaggtg aaagtacaat
    36241 agaagttgat tctatagaag attattttga aaaattagaa aaaaattata tagttttaga
    36301 ccaaaacaag agaaaagaaa tgataagaaa acaatgtata gaagtggcaa attctttagg
    36361 tggagaagtt gaatttgatg aagatttact tgaagaggta acttacttag ttgaatatcc
    36421 aactgctttt tatggagaat ttgatgtgga ttatataaaa ctaccaaaag aagttgttat
    36481 aactcctatg aaacaacatc aaagatattt tccagtactt aaagaaggaa aactgttacc
    36541 taactttata gctgttagaa atggtgattc ttacagaata gacaatgtta aagctggaaa
    36601 tgaaaaagtt ttagaagcaa gacttgcaga tgctttattt ttctatagag aagatactaa
    36661 gagaaactta gaaagctaca tagaaaaatt aaaaactgtt gtattccaag taaaactagg
    36721 aactatttac gacaaaactt taagacttga aaaattaagt gctgatatac ttgatagttt
    36781 aggtttagta gatgaaaagg cagatactat aagggctgct aaattatcta aggcagattt
    36841 agttactgga atggtaggag aatttgatga attacaaggt tttatgggaa aagaatacgc
    36901 aaaagttgga ggagaaaatg atgttgtaag tgaagctatt tttgaacatt atcttccaag
    36961 atttgcagga gatatacttc ctaagactaa tgcaggagtt gccttatcaa tagctgataa
    37021 gttggattca atagcaggtt tctttgctat aggaatacaa ccaacaggtt ctcaagaccc
    37081 atatgcttta agaagacaag ctttaggtat acttagtata cttatggaca gaaaagttgc
    37141 tgtggatata aaagtactta ttgaacatgc tttagacaat tattctgaat tagattttaa
    37201 taaagaagaa gttgttgagc aaataatgaa tttcttcaat gagagagtaa aaaatctatt
    37261 taaagattta ggaataagat atgatgttgt tgatgctgtt ttaagttctg atataaatga
    37321 tgtttctgat atgcatatga gagctttaga gcttaataat tggctagaaa aagatgaatt
    37381 agtggagatg cttactgcat ttaatagagt ttctactcta gctcaaaaag cagaaagtga
    37441 taaaattgat gaatctttat taaaagaaga tgctgaaatt aaactttaca atgaatttaa
    37501 acaagtaaag gtaaaagtta atgaattatt aaaggataag aaatatggta ttgctttaga
    37561 tgaatttgca tctttaagac cagtagttga taatttattt gatacagtta tggttatgga
    37621 taatgatgaa gctattaaga acaatagact tgctttactt aaacaagtat atgatactat
    37681 gctaggaatt tgtgatttat ctaaaattgt ttataaataa aatttaaact atttataaga
    37741 aataaggctg tctcaaaata gagatagtct tatttttcat tgttgagatg atgaaaatat
    37801 atttgatttc atagaaaaag agtgtctcat aaactaaaaa gttcattttg agacacccat
    37861 gctttttgta agtaaatata ataaattata ttttatactt atcagttatt ttgtttaaaa
    37921 tattatcatt tgaatttacc cattcataaa aattgcctga tgatgcgtcc gatattttat
    37981 aaagcatttc aggaatatca ctagctttaa aatctccaag tgattttccc aatctagtct
    38041 tacctatttc aaaactgcct ccaacaaata attcaaatgc atctgtagat ataccatcaa
    38101 cattcttctt tttaccacat aaacctatgc taccaacttg atgaacacca catgaattta
    38161 aacatcctga tatatataac ttaggtgctt tatttaaaat ttctttatta ttttgtaatc
    38221 taaaataatc tatgatttca tgtaacattt tctgactatt ttgtatgccc atttgacaaa
    38281 ttggaacacc tatgcaagat atactttgtt ctaattgaga attacaacta atagttttac
    38341 ttatttctaa tattcttttt gcttcatttc catttaaatt taaaatatat aatccctctg
    38401 tcattcctag tctaatcata ggattttcaa tattatctaa ttctttaagt aaagtactta
    38461 aatcctttgt taacaaaata cctccaactg gatgtatata tacagtgtat aaaccatcct
    38521 gtttttgagg tattagtcta gaatcctgta tatctatttt aaccccaggt ttagagtaat
    38581 ctatttcttc aatattgagt tcaagactac cattttcttt ttctaattta tagtattctt
    38641 taaatttttc taagaataat ttttcaccaa gtgattcgac catatatcta accctagctc
    38701 tgtttttatt ttcataattt ccataatcca taaagaattt aatcatacct tcaacacaat
    38761 ataaggcatc tttcggtttt ataagttcat caagttctaa agcaactttg ggttgtttac
    38821 ctagacctcc acctaagaaa actctgaaat atggttcatc atttttcaaa gtagcaacaa
    38881 atcctaagtc ttgtatacta caatgagcag tatctgtgta acaattagaa taagaaactt
    38941 ttaattttct aggtaaatgg tatgtagtag catttttgat aaaatattta tcagtagcaa
    39001 ctgcatatgg agtaacatca aagacttcac tagggtcaac tccagataat ggagataatc
    39061 cgacatttct aggataatta ccaccaccac ctctagtgaa tatatcattt tttattcctt
    39121 cttccatgat atcaacaatt gaatttatag ataaatcgtg taattgtata gcttgtctag
    39181 ttgttaagtg tatcttatct aagttgtgtt taatagctat ttcatagatt ttattaagtt
    39241 gatactgaga taaaactcca gcagatactc taagtcttat cataaaagat tttttgtctc
    39301 tttgagcata aacaccatat ccaccagaaa atcccttata ttgcatttta tttatttctc
    39361 catttaaaaa ttttgaagaa tattctttaa aattaggtag ttcatttaac aatatctttt
    39421 taaaattatc cattatttca cctcattgtt agtcttgtat aaaaatatat ctattgtata
    39481 atagatatat atattattat ctagtcagga taaaaatata acagaataat tctattaaaa
    39541 gtaagaataa taaggacata gtgagtattt tgtttttatt ttacaaatta agtataatat
    39601 ttttgtttta
//
LOCUS       DAMJVK010000030        38976 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.030, whole genome shotgun sequence.
ACCESSION   DAMJVK010000030 DAMJVK010000000
VERSION     DAMJVK010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 38976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38976
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.030"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(141..2021)
                     /locus_tag="PC414_001620"
     CDS             complement(141..2021)
                     /locus_tag="PC414_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp10"
                     /protein_id="HDF2464018.1"
                     /translation="MSKRKSFIRTIAVSTMAVAVTGSATCAYAAPVLQGTKTYEKVNT
                     IDISVDSVENIVYSFQASIKVQGEVEVVDNEQKEKITWSDNIKSQIKSGNANATCRAE
                     YNKSSNTTTLDIYVTSNEDLLDGNRLNIGRISVKKSGSNSNADYKVLGKGTNDKPALK
                     IVTYNNKTVDYENISSDEGLIFTLINESEVKPIGGTGSSKNDPEKYKVEKSEALEYLL
                     NNIRINYSIVSKETQESGSNVILKLGLAQKTTKGRKVTINKYVEVTLPKSLEYIVENE
                     LSKPDELPPDNGSGGNNGGGSNSGGSSSGGSSGGGNSSDSTSNVTVKKLKGDDRFETA
                     IKISQSGWTKSDTVVIVNGEDKSMVDGLTATPLASVKNSPILLSSNDKLPQKTVEELK
                     RLNPSKVVVIGGNNSMPNSVVEAIKAVNSKISVQRIGGDTRYQTSINIAKEIDKTNNV
                     SKLYIGAGNGEADSLSIASLAGKEKTPIVLTQKDGVDNEAEQFIKSNKVSNIYFIGGV
                     EKISNKAIEQVGKIANKDISNNRVAGQTRQETNAKVIDKFYSQSKLDGVVVANQDKLI
                     DALAVGPLAAKNNSPVILATNTLDKSQESSLKGKNSSKLFEVGGGIASSVIDKIKSLI
                     EK"
     gene            complement(2231..8194)
                     /locus_tag="PC414_001621"
     CDS             complement(2231..8194)
                     /locus_tag="PC414_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding adhesion protein"
                     /protein_id="HDF2464019.1"
                     /translation="MKKAISCVLAVSMCSAPLNVFAEPILEGKLRAVEKSVTEKLRGS
                     LEVDLNFSLPIKNSDSESMTNMQLRLKDDSNNSGIIKLGEKDSGTVNVGTDNIEYTIK
                     KLSASRSEIKNKDENISYYNIVFNNLPVGKYNVEVSGDGFKNKEIKDIDISSYSQRVL
                     LSNRASVDNKNKPLNYELFLMGDVNGDGKVDKSDYNKVLENIDSNKREFDLNRDGKVD
                     IVDLDYVQKNLGQSEDAKSLESIVSTNPIVDTSKVELKGNSDVDINGNVENLFSGENN
                     GVTISSKNDEISEEKPAVMDIALSEALNMEVINIKAPEGTAPAKGIVEVTDKDGNVQQ
                     IPYENKEYMKNLKDEEQQEEVNNEQEEVNQEEVNNKDENINQTDNNSNNKDENNDEQQ
                     ENSDKQEVNSNTNQDKNNDKENSSMDKPNSDEDTPKEEGNISKDDNSTTSTYSKSTLE
                     TYADVQNSDIVINLGKQVAVKKITIKITATTANDRNLAEISKVEFLNNVYKEIPKPEM
                     NIPKINKISTSTAVGNEKVSFYWNNEVNVSAYELILDKIDSKGKVLSTKKLQTSKNTI
                     EVRDIEAYGLYRVSIQSLNGDWSSGYKDATPENLDGVPENVDSNYKPIQFSPDSILEF
                     QVVPDTKPEPPEGINVKGKFKSLDVSWKNHKQAKDFDLYYKEKGSNGSWIKYNDKLIR
                     GTSSKIEGLKDNTTYEVRMTATNHLGTSGMSKTYIGTTESMDAPTTPNYRLINTPKEN
                     GGFGIPTNHIVDVTYPAGYDKSEYSDKNPFDKFNVVDGDFTTHWTFPTYNAAEGTNRG
                     PIITFDKSYTMDTFMATPRFDVSDKTINDFEVRYWDENGQLHNLGDVPFETKTDPVNN
                     NKYVMVRLKEPITATKIQVSVCSSYSYKSNISELKFYEYDSIEDDVRNLFMDDLQVEL
                     KEDVTQEKITELKDRLNTPDKASGEYHPYKTVIERELKLAQDLYNDRATLTDVTTVNQ
                     EINKLGKYKDKNGQYTVNSNNLGMQNDWQALGVAARAGDEITVYVGSKSGKTPKLIYT
                     QYYGESGAYKSGEINLNTGKNVIQLSKLHSLDIECGGSLYIRYAEDTPSGGDIKVRVA
                     GATKIPHLNLNGTINDKSPAGVSESKKKIKAYIEELARYKDAVKGEPYYPGTNVGNSY
                     GYSEKTGVLNTTDIESDKVTLNVPATAVYQGISKGNADLNTQVDRLYNSMLAWEQIMD
                     LVYSERGVFKTQDLDGNGTVDDNEFNLTKNDMAPKSRMNIKYQRMFIGAFMYASGLHV
                     GVEYGSVPGLMNGVPFQIDDSGKATGGNLFGWGIGHEIGHVTDIGKMTYSETSNNVLA
                     LLAQTFDDKTHSRIEGSTMDKIYEHVTSNSLGIPSNVFERLGMLWQLHLAYDDDFTGS
                     MLKNNSDADLSNDTFYAKISRKYRALSSSDPINSLPKDQMLVAMASSVVEKDLRDYFK
                     AWGVEITPELNSIMDSKNYEKESRKIQYVNDEARRKILNENIASMAKDTKVSASFSDG
                     IQSGSLVKSNKISIDLDVDKDKDKILGYEIIRSDGNYLDGENGTQVKYRTVGFVDAKG
                     NSKTKFTDNISPLNNRAFTYKVVAYDYHLNPTEEFEVGTVKLSDEGKINKSAWSFITN
                     TVSDGDVRTENDSHGPLQNPEIDNIKDNDANTTYKGKIISKAEWNKDPQKDPDINVEE
                     NPYIIVDMKETLPIVGLKYTKPETEARKFSLKGLFNFRKNTSTTYNPLTNYKIQVSND
                     KKNWEDVSQGTFEYGQDLVGGGKDQDNEARVTFNKDGKLWTYQARYVKLISNNKSNIE
                     VAELDIIGPPGDNIEIGTMNNADETRTNGIGKLAEDYVYQSDDSSTSEDETGKIPAGS
                     IVITGEYSGNPAFNLPLLIDKNNKTISGEALLFAEVPEKGELGEISKGTWVYYMPKEN
                     FDSLSDKVKAELYRYNDLQGDTPVGQRFVSDTMYVPVGAKNYDELKTISLTDSNSKAR
                     KATRNSIDISNKKVITVSNKVKGNIVRDK"
     regulatory      complement(8318..8403)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(8575..9978)
                     /locus_tag="PC414_001622"
     CDS             complement(8575..9978)
                     /locus_tag="PC414_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp9"
                     /protein_id="HDF2464020.1"
                     /translation="MKNSKKILAIGLTLFLVMVNTPMVNALTSVEQIKGNDRYETAAK
                     IADKQNYNTAILINSDNSLADGLSASGLAGALNAPILMTKQNQIPNTTMERLNKAKTV
                     YIIGSESTISKNVENQLLSKKKVVQRIFGENRFDTSIKIAEKIKEIKPIDKVIIANGF
                     TGEADAISASPVAARDGVPIILTDGNSVGFDTTGLKSYALGSSEIISDELVKSTNSIR
                     LGGTDRFETNKIVIQEFYKNSKEFYLSKGLQLTDALAASTIAKNAPVVLVENGSNKSI
                     LSGADKLTVLGGINQNVIKQCINQASPNQQGLYYNPNDRAFKERIKGKVYALTKQYRK
                     ENGVRALSVASRLEGLANDWSNLMANKKTLSHTINGKNSYSTFLKYLDWSEIKPGYIA
                     VQGENIIKYKIPDKPVYTNRDADDIGNFIFNEWKTNPEEGTNMLHKGYEIMGFGIAIT
                     GDKNLYATHEFYGRYKE"
     gene            complement(10440..12335)
                     /locus_tag="PC414_001623"
     CDS             complement(10440..12335)
                     /locus_tag="PC414_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp8"
                     /protein_id="HDF2464021.1"
                     /translation="MLSNKKRSMAIVMAGATVMSAAAPIFADNTVTENVDKNYTVSAK
                     DSAKLIEEVRKALEVKFEDTKAGANVNDRVYDIKVDNVNLTNATQLQNKINSLTEGQS
                     LKVTIQDKGHQVLGGKVVDYKIENYKTAQEIVDAVNAYNATLAEDSDNKLTATIKSTN
                     TVEVKRAKDSANVITLNVGDQHLDFSKVITSEEGTFEGYEKSYSDIDSKELHTITVKN
                     ADLQDISAEELFDGIRLTTLGREIVNKVKNGYALTFENEAILTQEQEDSDDKDKPEKS
                     SFDIVLSKANEKPETISVSSKNHKLVRDLHKVLTDVKGGKELKVEVLSGDSRFTTAVE
                     VSKERFKDGEAEAIILVGEDAIVDGLASAPLASQKNAPILLSKKDSLPSEIEAEILRV
                     LGSNLSSKKIYIVGGESKVSKETEEKLSKLGVSKVERVSGEDRFETSLEIAKQLKDTF
                     KTAFVVGGNGEADAMSISARAAQFGAPIIVTGNELDANAEKLLKGKELEIVGGENSVS
                     KEVEDKLVDIDLNNKVERLAGENRKDTNAKVINKYYAGATKAYVAKDGYVGGNGQLVD
                     ALTAAPLAASSKAPIVLTTEELSKSQEEVVELRLKNATKLVQIGEGIAKNAIEKIAEK
                     INLFTKN"
     gene            complement(12961..13104)
                     /gene="scfA"
                     /locus_tag="PC414_001624"
     CDS             complement(12961..13104)
                     /gene="scfA"
                     /locus_tag="PC414_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HDF2464022.1"
                     /translation="MENKHVKTLSQATLKQSAAKGGCGECQTSCQSACKTSCTVANQE
                     CER"
     gene            complement(13170..13532)
                     /locus_tag="PC414_001625"
     CDS             complement(13170..13532)
                     /locus_tag="PC414_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HDF2464023.1"
                     /translation="MGKSNYIFKGLGYAYIITLAVLLVYNLFLTFTDIGGDNITMVSS
                     FITTISAAIGGFYTSKHMKEKGLMYGLLVGLLYIVCIFLTVFLAQEKFVFEVGMIYKL
                     LLISAAGGIGGVLGVNFK"
     gene            complement(13590..13883)
                     /gene="yajC"
                     /locus_tag="PC414_001626"
     CDS             complement(13590..13883)
                     /gene="yajC"
                     /locus_tag="PC414_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDF2464024.1"
                     /translation="MPQQQIIMSIGLWVVVIAIFYFLMIRPQKKKDKQLKEMRSSLSV
                     GDKVITIGGIVANVAKVEDDVVILELGPNRTKVPFEKWAIGTVKSKKEEIEED"
     gene            complement(14017..15138)
                     /gene="tgt"
                     /locus_tag="PC414_001627"
     CDS             complement(14017..15138)
                     /gene="tgt"
                     /locus_tag="PC414_001627"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HDF2464025.1"
                     /translation="MYAVRYELIKTCKQSGARLGRLHTPHGIIETPIFMPVGTQATVK
                     SMTPEELKEIGSQIILSNTYHLYMRPGHELIKRAGGLHKFMNWDKPILTDSGGFQVFS
                     LGPLRKIKEEGVEFRSHLDGSKHFLTPEKAMEIQNALGSDIMMAFDECAPYPSDREYV
                     KNSLERTTRWLKRCKDAHNNTDKQALFGIIQGGMYKDLREQSAKEITNIDLPGYAIGG
                     LSVGEPKPLMYDVLEHTTPLMPKDKPRYLMGVGSPDDLVEGVIRGVDMFDCVLPTRIA
                     RNGTAMTSQGKVVVRNATYAEDFTPLDPECDCYACKNYSRAYIRHLIKANEILGARLI
                     TTHNLHFLLNLMKQIRQAIMEDRLLDFRNEFFAKYGYEI"
     gene            complement(15217..15702)
                     /locus_tag="PC414_001628"
     CDS             complement(15217..15702)
                     /locus_tag="PC414_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464026.1"
                     /translation="MHSLQKQKKNNPILKIAFIFERVLAVVVLIAVFLGTIDVLRLMW
                     GAYIVDFQNPVQYSQLNDFLAQILLLVIGVELVVMLSLHIPGAFIEALLYAIARKMLL
                     LPKNEGMIDVLIGVIAIAGLFAIKKFLVTKDKSALNIMSIHDEEAIKEQQNKEDEDKS
                     I"
     gene            complement(15714..16739)
                     /gene="queA"
                     /locus_tag="PC414_001629"
     CDS             complement(15714..16739)
                     /gene="queA"
                     /locus_tag="PC414_001629"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HDF2464027.1"
                     /translation="MKTSDFKFDLPQELIAQVPIEDRASSRLMVLDKETGNIEHKVFR
                     DIIEYLNPGDCLVLNNTRVIPARLIGEKLETGGKIEFLLLKRTEEDTWQALVKPGKRA
                     KVGTKFSFGNGKLIGEVVDLSDEGSRIIKFHYDGIFEEILDELGNMPLPPYITARLDE
                     KERYQTVYSKHNGSAAAPTAGLHFTEELLNKIKEKGVDIAFVTLHVGLGTFRPVKVED
                     VLNHKMHSEYYMVSQEAADKINRAKENGKNVICVGTTSCRTIESACNEDGKMKETSGW
                     TEIFIYPGYKFKVLDKLITNFHLPESTLIMLVSAICGKDNVLNAYNEAVKERYRFFSF
                     GDAMIIK"
     gene            complement(17057..18076)
                     /gene="ruvB"
                     /locus_tag="PC414_001630"
     CDS             complement(17057..18076)
                     /gene="ruvB"
                     /locus_tag="PC414_001630"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HDF2464028.1"
                     /translation="MQGFEDENRIITSTMKMEDIDIENSLRPKTLEDYLGQEKSKEQL
                     SIFIEAAKSRNEQLDHVLLYGPPGLGKTTLASIIANEMGVNLRITSGPAIERAGDLAA
                     ILTNLNENDVLFIDEIHRINRSVEEVLYPAMEDFCLDIIIGKGPSARSIRLDLPKFTL
                     IGATTRAGMLTNPLRDRFGVICKLDYYTVDELSKIVLRSSSILDAEIQSNGALELAKR
                     SRGTPRIANRLLKRVRDFAQVRADGKITDKVAKDALELLGVDSLGLDFVDEKLLMTII
                     EKFRGGPVGLDTLAASIGEDRNTIEDVYEPYLLQLGFINRGPRGRVAMPLAYEHLKIP
                     YPNEK"
     gene            complement(18183..18785)
                     /gene="ruvA"
                     /locus_tag="PC414_001631"
     CDS             complement(18183..18785)
                     /gene="ruvA"
                     /locus_tag="PC414_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HDF2464029.1"
                     /translation="MYSYIKGTVEEIYIDSIVVENNGIGYKINVSSNTIMNLQVGEAT
                     KIYTKLIVREDDMSLCGFVSREELKMFELLTSVSKIGPKVALSILSFASPAQLGAYIL
                     SEDIGKLSKAPGVGKKTAERIVLELKDKVDKNNIEFEPTLLSQKPTLISQDESVDALV
                     ALGYTLSESKEAVQKCKKDGMNTEAIIKKALTYIMSKSLK"
     gene            complement(18778..19287)
                     /gene="ruvC"
                     /locus_tag="PC414_001632"
     CDS             complement(18778..19287)
                     /gene="ruvC"
                     /locus_tag="PC414_001632"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HDF2464030.1"
                     /translation="MIILGIDPGIAIVGYGIIEYKNSKFKAIDYGAVTTPAHMNISRR
                     LELVYKGIDTIVKNYNIDEVGMEELFFNKNVKTAITVAQARGVTMLACAHNGKPVYEY
                     TPLQVKQGVVGYGRADKAQVQQMVTSFLSLKKVPKPDDVADALAVAICHAHSNKLEKT
                     LKNIGGKYV"
     gene            complement(19599..20228)
                     /locus_tag="PC414_001633"
     CDS             complement(19599..20228)
                     /locus_tag="PC414_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464031.1"
                     /translation="MFEKKKKELFPWKISALILVGVFVLSSGVYIGTRLKHNGVQTGE
                     VVSTSANMNEKSKEAFGLNKNCEVWLQSEGENETNYKRTLTMAGIVPESLLDKTKSEI
                     VSYFKKEYPTKKIKSMDKNEIILVESKYSDNKDVQAMDVTIKGKYTIENDNGNIALYK
                     YDDSGSKTLVEKTKIRVDSLPKTVQDEIKKGVVMDTEEEAYSRLEDFAS"
     gene            20431..20754
                     /locus_tag="PC414_001634"
     CDS             20431..20754
                     /locus_tag="PC414_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HDF2464032.1"
                     /translation="MERINCNVINCSHNNSHICYADKIFINGEKAHNDRHTCCSSFLD
                     ESIYSNLSSNVNNKNCEELVCTVKNCKYNKDGSLCTLDNILVAPETDRVNLYIETCCS
                     SFECK"
     gene            21022..21876
                     /locus_tag="PC414_001635"
     CDS             21022..21876
                     /locus_tag="PC414_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464033.1"
                     /translation="MSTFYIQFLYFVIYSFIGWCCETTYCSVLQKQFVNRGFLNGPFC
                     PIYGFGALAIVATLTPFVHNIPLLFLFSIIITSIMEYCTSFILEKIFNMTWWDYSKHK
                     FNIHGRVCLENSLMFGVLSLIVMLIVHPIVVDFINSISKNVLFIFAISIEIYFVLDLV
                     ITVHTILQLNGKLKQINLIIKELKDKKEYYKLITQETIENKLESLVENRLDTLEDRFE
                     ILKEKENYRYAKNRIDELKNKLNTLLSNHKLLHRRIIKAFPNISSNKHTDILNKIKEN
                     IKSIKKDK"
     gene            complement(22062..23498)
                     /locus_tag="PC414_001636"
     CDS             complement(22062..23498)
                     /locus_tag="PC414_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HDF2464034.1"
                     /translation="MREVKPISIDILNTFKQVDEDRLNKLLADELKHLDRKIVVLDDD
                     PTGVQTVHDISVYTDWDKDSMEQGFNEKNSMFFILTNSRGFTVAQTTKAHKEISKNIV
                     DVSKKVNKDFIIISRSDSTMRGHYPLETNLLKSEVERLSGKLFQGEIIMPFFKEGGRF
                     TIDNVHYVKEGEILVPAGMTEFAKDKSFGYKSSDIGEWCEEKTNGKYKSSDMIYISSK
                     ELRELNIEAITEKLKKAEGFNKIIVNATDYVDVKVFTIAFIRAVNGGKEFMFRSAAAI
                     TKVLGGVSDKELLTKDELVSKGNTNGGIILVGSHVNKTTQQLEELKNCKYPIEFIEFN
                     QHLVLQENGLKNEVKRVIKVVEEKISSGKTVAVYTRRERFDLDTNDKDKQLMISVEIS
                     DAVTSIIGMLNVRPNFIIAKGGITSSDVGTKALRVKKATVMGQIKPGIPVWMTGKESK
                     FPNMPYIIFPGNVGEISTLRESVEILMG"
     gene            complement(23569..24951)
                     /locus_tag="PC414_001637"
     CDS             complement(23569..24951)
                     /locus_tag="PC414_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDF2464035.1"
                     /translation="MITGYGLLIAFVISIGILLVSIIKFKVNPFLALLITSIITGFMV
                     RMPINEISTTISTGFGNTLGSIGIVIGLGIIFGNILSESRATESIAKGLLAKTGEKNS
                     ALAVTTAGFLISIPVFMDAAFVIMMPIIKYVSRVTKKSLMVFVCALGVGTIVGHALVI
                     PTPGPLAVAANVNANVGSFILYSIIVAFPAALIGGWIYGKRFEKYPAYAIDENDREKN
                     LEQGKDSIKIEDDDSSKVPGFGISMFSLLFPILLILISNVFSMFLEKGSTMSGVLAFI
                     GDKNIAILLGILVAIGFLKKYINKPMGDVVIEAADSAGLILLITGSGGAFGSVINASG
                     IGNFLVDTMSGLSISVVVLGFLLSALLRISQGSATVALVTTSSILGPTILATGMSPVL
                     VGLAICAGGVGFSLPNDSGFWVLSRFSGLSVKDTLNSWTIGGTIAGVVAFIMVLLLSV
                     INGIIPLPGL"
     gene            complement(25087..25992)
                     /gene="garR"
                     /locus_tag="PC414_001638"
     CDS             complement(25087..25992)
                     /gene="garR"
                     /locus_tag="PC414_001638"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HDF2464036.1"
                     /translation="MEGYKMKLGFIGLGIMGKPMAKNLLKDGCNLLVYDINKSAVDEL
                     ISCGAKYASVLEMGQECDIVFTILPNGTIVQDILFGMDGLAKTLKEGSIVVDMSSVTP
                     TESILCANKLKDMGLEFIDSPVSGGEPKAIDGTLAFMAGGKEEIYKKVEPFFNIMGSS
                     SILIGDNGSGSVTKLTNQVIVNLTIAAVSEAFVLAAKAGADPEKVYKAIRGGLAGSTI
                     LDVKIPMIMNRDFKPGGKISINLKDIKNVMQTAHNLDVPLPMTSQLLEIMQTLKVHGH
                     LEDDHSGIAQYFEKLAGVEIKKHDN"
     gene            26388..27059
                     /locus_tag="PC414_001639"
     CDS             26388..27059
                     /locus_tag="PC414_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2464037.1"
                     /translation="MATFKRLQEQAYDYLKELILSGEMIENEIYSETKLASEIGISRT
                     PIRDALQRLSQDGFIDIIPSKGFRIHQITANEIVEIFQIRSAIEGFCTFLITSQYKEA
                     RAVETISKLKHLLDKQKDILLGDKNLNSFAEYDTLFHTTIVSYAQNTEFDKMFNNYMY
                     RIKKLALDSLSHEGRLETTLKEHTDIFNNIANGCIEDIYRTTLIHMETPKYINLEDFC
                     NNKLF"
     gene            27162..27710
                     /locus_tag="PC414_001640"
     CDS             27162..27710
                     /locus_tag="PC414_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464038.1"
                     /translation="MIFLNEDVVTNETIDKDYAIEEVRMACKHFGDLYFYFSKVLFEE
                     FGEDKTSEILRKVLFERSEERAIAMRERAHENGDELIADNIISTTDVPFLGWVPEFEE
                     LHCPYGASWLLRFEENPWFKKFASLYCDVTDTTVAEVFTGDTSHKITKNILWGDKSCE
                     RIYFHDDKVSEGKYTYGNKNCK"
     gene            27972..28280
                     /locus_tag="PC414_001641"
     CDS             27972..28280
                     /locus_tag="PC414_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464039.1"
                     /translation="MFNKIYEKKEFIVFQVKKGYVVYNTRKSFEEGHTHLKHFEAAKT
                     AIDLAINKKIPRSKDGYYLTSLIRISDDGYYIDKLSELLYVREQKGKKEKYCNSGYQM
                     "
     gene            complement(28442..30232)
                     /locus_tag="PC414_001642"
     CDS             complement(28442..30232)
                     /locus_tag="PC414_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2464040.1"
                     /translation="MKKSKGFKKTIGRLLPFVKKYKFSFIIAIICIISAATMNALAPK
                     TEGLIITQLTKDVISIAKGVPGASVNFDYVTKILVILACIYFANAIFTYTSSFLLTNA
                     IQNTMRDLRNEVENKIRRLPISYFDSNSFGDVLSRISNDVDTISNALQQSFMQIVNSI
                     LVIILALSMMFTINIYMALIALFIIPISYFVSKFVVKKSQSRFSLQQNALGKLNGKVQ
                     EMYTGFNEIKLYGKEEDSIKEFKKVNQELCENGFKAQFISSMMNPMVSLVTYFGIAAV
                     AVVGSIYAVSGGITVGNLQAFVRYIWQINQPLSQMTQLSTVIQSSFAAIERVFEILDE
                     EEEIPDVENPVKIENVKGNVTFEHVNFGYGENSTLIEDLNAEVKSGQMVAIVGPTGAG
                     KTTLINLLMRFYDVKKGAIKIDGVDIRDMKRKDLRSMFGMVLQDTWLFNGTIFENIEY
                     GRFGATKEEIIQAAKVANVHHFITTLPDGYNMFLNEEASNISLGEKQLLTIARAFISD
                     PSILILDEATSSVDTRLELMLQKAMRNLMNGRTSFVIAHRLSTIRNADLILVMNNGSI
                     IEQGNHDELMEKGGFYEKLYNSQFADKESE"
     gene            complement(30232..31959)
                     /locus_tag="PC414_001643"
     CDS             complement(30232..31959)
                     /locus_tag="PC414_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003496244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2464041.1"
                     /translation="MKLILSYLKNYKLLIVLNILAIFSFALVELGIPTIIAKIIDNGI
                     ANQNIAYIKQMGIVIVIISIIGVVGSILLGYCSAKVSTSVTRDIRNDIFEKSQEFSHT
                     EYNKFGISSMITRTTNDAFQIQQFVNILLRTALLTPVMFIISIIMTIRTSVELSIVLA
                     ISVPFIIIGVAIIAKVSQAISSKQQKGLDKLNLISRENLTGIRVIRAFGNDDYETERF
                     EKTNTYYANVSKKLFKLMSITQPAFFLLLNIAVLAVFWISSEKINIGELQVGQLVAFL
                     EYLFHAMFSIMLFSMVFIMYPRAEVSANRIKELLNEEPLIKNPENGIKDTENKGIIEF
                     DNVTFTYPDGEASVLKDISFTAKTGETVAFIGSTGSGKSTLINLIPRFYDVTEGSIKI
                     NGVDIREYDLKALRKKIGFIPQKSLLFTGSIANNIRFGKHKAGESELEYSAKVAQAYE
                     FISKKPRKFDELISEGGANVSGGQKQRLSIARAIIRRPEIYIFDDSFSALDFKTDAIL
                     RAKLKKETKDAIVLIVAQRISSIIDADKIIVLNEGQVVGMGTHKELLKNCEIYYEIAT
                     SQLKKEELE"
     gene            complement(32204..32470)
                     /locus_tag="PC414_001644"
     CDS             complement(32204..32470)
                     /locus_tag="PC414_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HDF2464042.1"
                     /translation="MNLEPKKLIEQIGKEKDKKDIWIVGGGDIVKLFMKDNLIDDYYI
                     YILTIILGRGISLFKSGIDKTNLNFKKASNIGELVKLEYSKKQK"
     gene            32768..33880
                     /locus_tag="PC414_001645"
     CDS             32768..33880
                     /locus_tag="PC414_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDF2464043.1"
                     /translation="MKISEVATPNFLLDLDQLEKNIDKIQQICTNNNKQLWPMLKTHK
                     STYIARLQKNAGASGFLVGTLDEGEALIKAGFEDIMLAYPYMGEVNINRIVTMASKIN
                     LICATDNIECAKAYSEAFSKSKIECKLLIIVDCGLHRFGVEPEKVVELATEISKLENI
                     KIVGISSHPGQVYGCSSPEGVPDVCVQEDSSMEKAYKALTDNGFNIEIVASGSTPTVE
                     SEAASKTITAVRPGNYVYYDAIQIGLGCATEDMCALTVVCTVISKNSSGYYLIDCGSK
                     CLGLDKGAHGNSSIVGYGRVCGHPELTIDSLSEEVGKIKVNGETDIKIGDRIRIIPNH
                     SCSTANLTSNLLGFRGDTIEKVIEVDIRENSKQIIL"
     gene            complement(34244..34753)
                     /locus_tag="PC414_001646"
     CDS             complement(34244..34753)
                     /locus_tag="PC414_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HDF2464044.1"
                     /translation="MSQQNTGNVIVNSKPKKTAVEIFMGGAKKGFYIGVEQILPAMIM
                     GYVIVQFLQLTGLVDILGNIFGPVMGIFGLPGESVVVLIAAFFAKAAGAATAANLFAQ
                     GLITAPQATILIMPCMLMGTLVGHYARIVLVANASTKYRMLLLAVPIFDAIVGMLIVR
                     VLLTVMGLM"
     gene            complement(34755..35402)
                     /locus_tag="PC414_001647"
     CDS             complement(34755..35402)
                     /locus_tag="PC414_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464045.1"
                     /translation="MDVTAGDNKVTWKGYLSLFVLIVLFSGVFKDSQGFLKALDFANL
                     TGSFGTIADGVDFMGKGGTGAKDGFLFALTLIPTVCFAVGLIDVVESMGGMKAASKIF
                     NPLLRPLLGIPGIAGIAFVSSFTSSDVASIMTKELFESGEITDDERTIFVAYQYAGSA
                     VILNTINTQAPLLPIALLALGPIILIEIFCKILGANLVRLIIGVRNKKNKAKEAV"
     gene            complement(35416..36588)
                     /locus_tag="PC414_001648"
     CDS             complement(35416..36588)
                     /locus_tag="PC414_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2464046.1"
                     /translation="MNTYLNEISKFVDENREEIVSLWKEIVNMESYTHCKESVNKLAK
                     RLKFEFEKEGLDCELVDVGDNGSTLIGTLGSNIDKKPIIFSGHMDTVFETGTFGENPF
                     KIIEGKAYGPGVLDMKGGIIISLYAIKALNKIGYKERPIKIVFSGDEEIGHKDSTGAD
                     VILREAKGGLCAFNMETGLVDNSLCVGRKGRIGCNIHVKGVETHAGNDFEGGRNAIEE
                     MANKILRIQKLTNLDVGTTVSVSVIKGGRVENSIPEDCSIKVDLRFEKVEEMENVKEQ
                     VREICKETYIEGTSTEVNFISEMMPFETTEDVMRFHTFVNEVSKENGFGELNAKRLGG
                     SSDASYLTIANVPTICSFGVRGEWNHTSREYAVVDSMFERVKLISTVILNLDKFEV"
     gene            36856..38232
                     /locus_tag="PC414_001649"
     CDS             36856..38232
                     /locus_tag="PC414_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464047.1"
                     /translation="MKYKIGFLTTSYFMSDKFKQALKELNDTCEITFIELKGENPFNN
                     LPNIYYEQVDNFDGFVCSGIVPYSELIINIKDIKVPLNFLKLDEKDFYKYLFKLLNSQ
                     KDIDFTKSFMDFLREDNNYYDIYSLIDSNKCPYTIKDFNIPQPVYDFKKLNDKLLEIH
                     LDLLRDNKINLSFTRNYIISCELNKLGYDSIYMVPSTESILNTFKSLVNEITLQNLDK
                     NKSATCIVTVNSSEYINEDLIFKNDEIQNQIYNTILNSLSRHGFYGVQVKKNDMKIEI
                     HTTKEMLNNMTNNYTDFFISEDLKEIKYSLNIGWGIGNTNIHAEQNARQANGKSASLN
                     GNCTFIVNDTNDTIGPLYSNKNSNNIEDSSIANKVASFIPLSNTNVSKIMCMINDRNS
                     NNVSAEILADYMNITLRSANRILSILYKAGIATIVNTKLDNQRGRPKKIYKVDFLSFL
                     NKMQKLNS"
     gene            complement(38482..38841)
                     /locus_tag="PC414_001650"
                     /pseudo
     CDS             complement(38482..38841)
                     /locus_tag="PC414_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891108.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
BASE COUNT        12419 a         6798 c         4478 g        15281 t
ORIGIN      
        1 taaacttaat atagctcaaa ttattataca ttttaacctc aatattatta gattgttaag
       61 ataatgaaaa taaaataaga atgcatttaa atatataact ttatatctaa atacattctt
      121 attttctatt tacttaatat ttatttttca attaaactct taatcttgtc tattacagat
      181 gaggctattc ctccacctac ttcaaacaac tttgatgaat ttttaccttt taatgacgat
      241 tcttgtgatt tatctaacgt atttgtagca agtataactg gtgaattgtt tttagctgct
      301 aatggtccaa ctgctaaagc atctataagt ttatcttgat ttgcaactac aactccatct
      361 agcttagatt gagagtagaa tttatctatt actttggcat tcgtctcttg tctagtttga
      421 cctgccaccc tattgtttga tatatctttg tttgctattt ttccaacttg ttcaatagcc
      481 ttgttagata ttttttctac tccaccaata aaatatatat ttgacacctt atttgattta
      541 ataaattgtt cagcttcatt atctacacca tctttttgtg taagtactat tggagttttt
      601 tctttaccag ccagtgatgc tattgataat gaatcagctt ctccatttcc tgctcctata
      661 tacaacttgc ttacattatt cgtcttatct atttcttttg ctatattaat agaagtttga
      721 tatcttgtat ctccacctat tctttgtact gatatttttg aatttacagc tttgatagct
      781 tcaacaactg aatttggcat tgagttgttt ccacctatca caactacttt tgatggattt
      841 agccttttca attcttctac agttttttga ggaagtttat catttgatga taaaagaatc
      901 ggtgagtttt ttacactagc aagtggtgta gctgtaagcc catccaccat acttttatct
      961 tctccattta ctattacaac tgtatctgat tttgtccaac cactttgaga tattttgata
     1021 gctgtctcaa atctatcatc accttttaat tttttgactg tgacattaga tgtagaatca
     1081 cttgaatttc ctcctccaga gcttccacca ctagatgagc cgcctgaatt acttcctcca
     1141 ccattatttc caccactacc attatctggt ggtaattcat ccggttttga taattcattt
     1201 tcaactatgt attctaaact ttttggaagt gtaacttcaa catatttgtt tattgtaact
     1261 tttcttccct tagttgtttt ttgtgctaat ccaagtttta atattacatt tgaaccactt
     1321 tcttgtgttt ctttactaac aatagaatag tttatcctaa tattgtttaa taaatattca
     1381 agtgcttcac ttttctctac cttatacttt tcagggtcat ttttactaga accagttccc
     1441 ccaatcggtt ttacttcact ctcattaata agggtaaata ttaatccttc atcagagctt
     1501 atattttcat aatccactgt tttgttgtta tatgttacaa tttttaaagc tggtttatca
     1561 tttgttcctt ttcctaaaac tttatagtca gcatttgagt tacttccaga tttttttaca
     1621 gatattcttc ctatattcaa tctatttccg tctagtaagt cttcatttga tgtaacatat
     1681 atatctaatg tagttgtatt acttgatttg ttgtattctg ctctacaagt agcatttgca
     1741 tttccacttt ttatctgaga ttttatatta tcagaccaag taatcttttc tttctgctca
     1801 ttatctacta cttcaacctc accttgtact tttatactgg cttgaaatga atatactatg
     1861 ttttctactg agtccacaga tatatctata gtatttactt tctcatacgt ttttgttcct
     1921 tgtaaaactg gagctgcata tgcacatgta gcactaccag ttacagctac tgccatagtt
     1981 gatactgcta tagttcttat aaaagatttt cttttactca ttaacacccg tccttatcta
     2041 tcattaacta agtggataaa tttacttatc cacctagctt ttatatttaa tttataaatt
     2101 accctaaatt tatgattaca tttatatact ttacttttaa tatctactat tttaaaacta
     2161 ttctagtacc taatcttatt agatatacaa tgttagtgtt agtattttta cgtttttaga
     2221 gttccaccta ctatttatct ctaacaatat taccctttac tttattgctt acagttataa
     2281 ccttcttatt actgatatca atagaatttc ttgtagcttt tctagcttta ctattactat
     2341 cagtaagact tattgtcttt aactcatcat aattcttagc tcctacaggt acatacattg
     2401 tatcacttac aaatctttga cctactggag tatctccttg aaggtcattg tatctatata
     2461 gttcagcctt aaccttatca cttaaagaat caaagttttc ttttggcata tagtaaaccc
     2521 atgtaccttt actgatttca cctagttcac ctttttctgg aacttctgca aataaaagtg
     2581 cttctccaga tatagtctta ttgtttttat ctataagtag tggtaaatta aatgctggat
     2641 ttccactgta ttcacctgta ataactattg aacccgctgg aatcttacct gtttcatctt
     2701 cacttgtgct agagtcatca ctttgatata cataatcttc tgctaatttt ccaattccat
     2761 tagtacgagt ttcgtctgca ttattcattg tgccaatttc tatattatca cctggaggtc
     2821 ctattatatc taattctgca acttctatat tagacttatt atttgagatt agttttacat
     2881 atctagcttg gtatgtccaa agtttaccat ctttattaaa tgtaactcta gcttcattat
     2941 cttggtcttt tcctccacca actaaatctt gtccatattc aaaagttcct tgacttacat
     3001 cttcccagtt ctttttatca ttacttactt gtattttgta attagttaaa ggattgtatg
     3061 ttgtgctagt attctttcta aagttaaata atccttttaa tgagaacttc ctagcttctg
     3121 tctctggttt tgtatatttt agtcctacta taggtaatgt ttctttcatg tctactatta
     3181 tatatggatt ttcctctaca tttatatcag ggtctttttg agggtctttg ttccactctg
     3241 ctttacttat tatctttcct ttatatgtag tatttgcatc attatctttt atattatcaa
     3301 tttcaggatt ttgtaatggt ccatgtgagt cattttcagt tctaacatca ccgtcagaaa
     3361 cagtatttgt tatgaatgac catgctgatt tatttatttt cccttcatct gataatttta
     3421 cagtaccaac ttcaaattct tctgttggat ttaggtgata atcatatgca actaccttat
     3481 atgtaaatgc tctgttatta agaggagata tattatcagt aaatttagtc ttactatttc
     3541 cttttgcatc cacaaatcca actgttctgt attttacctg tgttccattt tcaccatcta
     3601 aataatttcc atcacttctt attatttcgt atcctagtat cttatcttta tccttatcta
     3661 catctaggtc tatagatatt ttattagatt taactagaga accactttgt attccatcag
     3721 aaaaactagc acttacttta gtatcttttg ccatactagc tatattttca tttaaaatct
     3781 ttcttctagc ttcgtcattt acatattgta tctttcttga ttctttttcg tagttcttac
     3841 tatccattat actgtttaat tctggtgtaa tttctactcc ccatgcctta aagtaatctc
     3901 ttaaatcttt ttcaacaaca cttgatgcca ttgctactag catttggtct tttggcaaac
     3961 tattaattgg gtcactactt gaaagtgctc tgtattttct tgaaatcttt gcatagaatg
     4021 tatcattact taaatcagca tctgagttat tctttaacat tgaacccgta aaatcatcat
     4081 cataagctag atgtaattgc cataacattc caagcctttc aaatacattg cttggtatac
     4141 ctaagctatt tgaagttaca tgctcatata tcttatccat tgttgaacct tcaattcttg
     4201 aatgtgtctt atcatcaaag gtctgagcaa gtaaagcaag tacattgtta ctagtttcgc
     4261 tatatgtcat ctttccaata tctgtaacat gtcctatctc atgacctatt ccccatccaa
     4321 ataagtttcc tcctgtagct tttccactat catctatttg gaaaggtact ccattcataa
     4381 gccctggaac tgaaccatat tctactccta catgtaatcc agaagcatac ataaatgctc
     4441 caataaacat tctttggtat tttatgttca tacgagattt tggagccata tcatttttag
     4501 ttaagttaaa ttcattatca tctacagttc cattaccatc taaatcttgt gttttaaata
     4561 ctcctctttc tgagtaaact aaatccatta tttgttccca tgcaagcata ctattatata
     4621 atctatctac ttgtgtattt aaatcagcat ttccttttga tatcccttga tatacagctg
     4681 ttgcaggaac atttaatgtt actttatcac tttcaatatc tgtagtgttt aacacaccag
     4741 ttttttctga atatccatat gaattaccaa catttgttcc tggataatat ggttctcctt
     4801 ttacagcatc tttatatcta gctaattctt ctatatatgc ttttatcttt ttcttagatt
     4861 cagaaactcc cgcaggactc ttatcattta ttgttccatt tagattaagg tgtggtatct
     4921 tagtagctcc tgctacacgt acttttatat ctcctccact tggagtatct tcagcgtatc
     4981 ttatgtataa acttcctcca cattctatat ctaaactatg aagtttgctt aattgaatta
     5041 cattttttcc agtgttaaga tttatttctc cactcttata tgcaccagat tctccatagt
     5101 actgtgtata tatcaactta ggagtttttc cacttttact tccaacatat actgtgattt
     5161 catctcctgc tctagcagct actcctaatg cctgccagtc attttgcata cctaaattat
     5221 tggaatttac agtatactgt ccgtttttat ctttatattt tcctaattta tttatttctt
     5281 gatttacagt agttacatca gtaagtgtgg ctctatcatt atataagtct tgagctaatt
     5341 ttaattctct ctcaataact gtcttatatg gatgatattc tccactagct ttatctggag
     5401 tatttaatct atcttttaat tcagttattt tttcttgagt tacatcttct tttaattcaa
     5461 cttgtaggtc atccataaat agatttctta catcatcttc tatactgtca tactcataaa
     5521 acttaagttc tgatatatta gacttatatg aataggatga acaaacactt acttgtatct
     5581 tggtagcagt tattggttct tttaatctaa ccattacata cttattatta tttacagggt
     5641 ctgtctttgt ttcaaaagga acatccccca gattgtgtaa ttgaccattt tcatcccaat
     5701 atcgtacctc aaagtcattt attgtcttat cagaaacatc aaaacgaggt gtagccataa
     5761 aagtatccat agtataagat ttatcaaaag ttattattgg tcctctattc gttccctcag
     5821 ctgcattata tgttgggaat gtccaatgtg ttgtaaagtc cccatctact acattgaatt
     5881 tatcaaatgg atttttatca ctatattcac ttttgtcata tcctgctgga taagtaacat
     5941 ctacaatgtg atttgttggt attccaaatc caccattctc tttaggagta ttaattaatc
     6001 tatagttagg tgttgttggt gcatccatgc tttcagttgt acctatatat gttttagaca
     6061 ttccactagt tcccaagtgg ttagtagctg tcattctaac ttcatatgtt gtattatcct
     6121 taagaccttc tatctttgaa cttgtcccac gaataagctt atcattgtat tttatccaag
     6181 acccattaga gcctttttct ttatagtaca agtcaaaatc tttagcttgt ttatgatttt
     6241 tccagcttac atcaagagat ttaaatttac ctttaacatt tataccttct ggtggttctg
     6301 gttttgtgtc tggaacaact tgaaattcta atatactatc tgggctaaat tgtataggtt
     6361 tatagtttga atctacattt tctggtactc cgtctaagtt ttctggtgtt gcatctttat
     6421 aaccactaga ccaatctcca tttaaagatt gtatgctaac cctatatagt ccatatgctt
     6481 ctatatctct aacttctatt gtatttttac tggtttgtaa ttttttagta gataatactt
     6541 ttcccttact atcaatttta tctagtataa gttcgtatgc tgatacattt acttcattat
     6601 tccaataaaa agatactttt tcatttccaa cagcagtgct agtcgatatt ttatttattt
     6661 taggtatatt catctctggc tttggtattt ctttatatac attgtttaaa aattcaactt
     6721 ttgatatttc tgcaagattt ctatcgtttg ctgttgtagc tgttattttt atagttatct
     6781 tttttactgc tacttgttta ccaagattta ttactatatc actattttga acatctgcat
     6841 atgtttctaa tgttgactta ctatatgtag atgttgtaga gttatcatct ttactgatat
     6901 taccctcttc ttttggagta tcttcatctg aattaggttt atccattgat gaattttctt
     6961 tatcattatt tttatcttga ttggtattag agtttacttc ttgtttatcg ctattttcct
     7021 gttgttcatc attattttca tctttattat ttgagttatt atcagtctgg tttatatttt
     7081 catctttatt atttacttct tcttgattta cttcttcttg ttcgttattt acttcctctt
     7141 gttgctcctc atcttttaag tttttcatat attctttgtt ttcataagga atctgttgaa
     7201 cattaccatc tttgtcagta acctcaacta ttccttttgc tggagcagta ccttctggag
     7261 cttttatatt gatgacttcc atattaagtg cttctgataa agctatatcc attactgctg
     7321 gtttttcctc agatatttca tcattttttg aagatattgt aactccatta ttttctccac
     7381 taaataaatt ttctacatta ccattaatat ctacatcact atttcctttt aattcaacct
     7441 tacttgtatc tactatagga ttggtagata ctatactttc taggcttttt gcatcttcac
     7501 tttgtcctaa atttttctgt acataatcta agtcaacaat atctaccttt ccatctctat
     7561 ttaaatcaaa ttctctttta tttgaatcta tattctctaa aaccttatta taatcactct
     7621 tatctacttt accgtctcca tttacatcac ccattaagaa caattcataa tttaaaggtt
     7681 tatttttatt atctacactt gctctattgc ttaataagac tctttgtgaa taacttgata
     7741 tatcaatgtc tttaatttct ttgttcttaa atccatctcc agagacttct acattgtatt
     7801 ttccaacagg taaattatta aatacaatat tataatatga tatattctca tctttatttt
     7861 ttatttcact tctacttgca gataattttt ttatagtata ttctatatta tctgtcccaa
     7921 catttactgt tcctgaatct ttttctccaa gctttattat tccactattg ttactatcat
     7981 cttttaatcg taactgcata ttagtcatac tttctgaatc gctatttttt attggcagtg
     8041 aaaaatttaa atctacttca agactccccc ttaacttttc tgtaactgat ttttcaactg
     8101 cccttagctt cccctctaat ataggttctg caaaaacgtt tagtggtgca ctacacatag
     8161 atactgctag tacacaagat attgcctttt tcatgaaata acaatcctcc cttaataaat
     8221 atctaatttt tcatgaattt aaatactata agtttcagac aataaaaaaa gacactcaaa
     8281 gagaatctta attataaaat aaaaagactt tactcttttt agcaactggc tgatatggct
     8341 ttaaaagctt taatccctat agttttgcgt cattagattt ccctaatttt gccatttaat
     8401 tttcactact ttcttaccca ttttatacta tatttaaact ttttaataca atttttttag
     8461 attaaattat aaaaaaataa catttatatt aatttattaa tataaatgtt attttccatt
     8521 ataatttaat tatttatata gcttccttac aagcataatt ttgtgtatat tttcttattc
     8581 tttatatctt ccataaaatt catgtgtagc atatagattc ttatctcctg ttatagctat
     8641 accaaatccc attatttcat accctttatg caacatgtta gttccttctt ctggatttgt
     8701 cttccactca ttaaaaataa agtttcctat atcatctgca tctctatttg tatatacagg
     8761 tttatctgga attttatatt taattatatt ttcaccttga actgctatat atccaggttt
     8821 aatctcactc caatctaagt atttcaaaaa agtagaatat gaattttttc catttatcgt
     8881 atgagataaa gtcttcttat ttgccattaa attagaccaa tcatttgcta aaccctctaa
     8941 tctgcttgct acagataacg ctcttactcc attctctttt ctatactgct ttgtaagagc
     9001 atatacttta ccctttattc tctctttaaa tgctctatca tttggattgt aatataaacc
     9061 ttgttgattt ggtgaagctt gatttataca ttgcttgata acattctgat ttatgcctcc
     9121 tagtacagtc aatttgtctg caccacttaa aatactcttg ttactgccat tttcaactaa
     9181 aactacagga gcattttttg ctattgtaga ggcagccaaa gcgtctgtta attgtaagcc
     9241 tttacttaga taaaattctt tactattttt ataaaactct tgtataacta tcttattagt
     9301 ttcaaatcta tctgtgccac ccaatctaat agaatttgtg ctcttaacta attcgtcgct
     9361 gattatttca ctagaaccaa gcgcataact ctttaagcct gtcgtgtcaa atcccacact
     9421 atttccatct gtaagtatta taggtactcc atctctagca gctactggtg atgcacttat
     9481 cgcatctgct tctcctgtaa atccattggc tataattact ttatctattg gcttaatttc
     9541 tttgattttt tcagctattt ttatacttgt atcaaatcta ttttcaccaa agattctttg
     9601 tacaaccttc tttttggata gtaactgatt ctcaacattt ttacttattg ttgattctga
     9661 gcctattata tacacagtct ttgctttgtt taatctttcc atagttgtgt ttggaatctg
     9721 attttgtttt gtcatcaaaa taggcgcatt taaggctcca gctaaaccac ttgcacttag
     9781 accatctgct aagctattat ctgagttgat tagtattgct gtattgtagt tttgcttatc
     9841 tgctattttt gctgctgtct catatctgtc attccctttt atttgctcaa ctgatgtcaa
     9901 cgcatttacc ataggagtat ttaccattac taaaaatagt gtaagtccta tagctaatat
     9961 ctttttacta ttcttcatat tattttctac ccccaatgta cttttcatat tttttattag
    10021 tactcatact tatttttaat caaaagttac aattttgtta ccatatttta tatatgatac
    10081 catagttatc catacaactc aaacaatata cctaaagttt ttattctcta taaattcaaa
    10141 acattataat ttattttatt taattaagtt ataagttcaa aataatttat gttaataaga
    10201 tgtttaatca ttataagaag tatcttttta taataatttt tgcaatatga atttctcaac
    10261 attataatca aaaaataaaa taaggtatct caatctaatt ctaaatgaga caccttattt
    10321 ttattatttt atcttgttat ttgtatattt tataatatct acactatgtc ttacatatta
    10381 acgcattgta taatcatata ttatctaaac aaacttttaa tattaattcc tatatcttat
    10441 tagttcttag taaataagtt tattttttct gctatttttt caatagcgtt tttagctatt
    10501 ccttcaccta tttgtactaa cttagtagca ttcttaagtc ttaactcaac tacttcttct
    10561 tgtgatttag aaagttcttc tgtagttaat actattggag cctttgaact agctgcaagt
    10621 ggtgctgctg taagtgcatc aactaattgt ccatttccac ctacataacc atcttttgct
    10681 acatatgctt tagttgcacc tgcatagtat ttattgatta ctttagcatt agtatctttt
    10741 ctattttctc cagctaatct ttcaacttta ttatttaaat ctatatcaac taatttgtct
    10801 tcaacttctt ttgatacaga attttctcca cccactattt ctaattcttt tccttttaat
    10861 aatttttcag catttgcatc taattcgtta cctgtaacta ttattggagc accaaattga
    10921 gcagctctag ctgatatact catagcatca gcttctccat ttccacctac tacaaaagca
    10981 gtcttaaatg tatcttttaa ttgttttgct atttctaaag aagtttcaaa tctatcttct
    11041 ccagaaactc tctcaacttt acttacacct aatttagaaa gtttttcttc agtttctttt
    11101 gatactttag attctccacc tactatatat attttcttag aagatagatt acttccaagt
    11161 actcttaata tttcagcttc tatttctgat ggtagtgaat cttttttaga taataatatt
    11221 ggtgcatttt tttgagaggc aagtggtgct gatgctaatc catcaactat agcatcttct
    11281 ccaactaaaa ttatagcttc tgcttctcca tctttgaatc tctctttact aacttctact
    11341 gctgttgtaa atcttgaatc accagataaa acttctactt ttaattcttt tccacctttt
    11401 acatcagtta atactttgtg taagtctcta actaacttat ggtttttact tgaaacactt
    11461 attgtttctg gcttttcatt tgccttgctt aaaactatat caaagcttga tttttctggt
    11521 ttatctttat catcactatc ttcttgttct tgagtaagga ttgcttcatt ttcaaatgtt
    11581 aaagcatatc catttttaac tttattaact atttctcttc ctaatgtagt taatctaatt
    11641 ccatcaaata attcttctgc tgatatgtct tgtaaatctg cgtttttaac tgttatcgta
    11701 tgtaattctt ttgaatctat gtcactataa ctcttttcat aaccttcaaa agtaccttct
    11761 tctgatgtta tcacttttga gaaatctaaa tgttggtctc ctacatttaa tgtaataaca
    11821 tttgctgaat cttttgctct ttttacttca actgtatttg tagattttat agttgctgtt
    11881 aacttgttat cagaatcttc tgctaaagtt gcattgtaag cattaactgc atctactatt
    11941 tcttgagcag ttttgtagtt ttcaatttta tagtcaacta cttttccacc taacacttga
    12001 tgtcctttat cttgaatagt aacttttaaa ctttgccctt ctgttaaaga gtttatctta
    12061 ttttgtaatt gagttgcatt agttaagttt acattgtcta cttttatatc gtaaactcta
    12121 tcatttacat ttgctcctgc ttttgtatct tcaaacttaa cttctaatgc ttttctaacc
    12181 tcttcgatta gtttagcaga atctttcgca cttactgtgt aatttttatc aacattttct
    12241 gtcacagtat tgtctgcgaa tattggtgct gctgcactca taactgtagc acctgccatt
    12301 actattgcca tacttctctt tttgttgctt aacatattta tgtcctccga atattatttt
    12361 ttatttttta tattcgtatt tcccacacat aaataatatt atatataata ttatttttca
    12421 atatatttta tgtgtttaca taatttacta acacaaacac taataatatc ttcttgaacc
    12481 ccttccccct gcttagctct ctataattat gctcaaagat agaaatccta ttttatagtg
    12541 attttctact aaacaatcat atactaacaa aagtattcaa actccatttt tatatactac
    12601 tatgcactaa tttttgcttt actcttatta cttaaatata tatcactaaa ttaatatcta
    12661 tctccatata ttaaaatata taacatatta taaaaatcat cataaattag agtttaaaat
    12721 taaaaattat tctaatacat gtaattgata aaaaataaaa tataagagta gctactttaa
    12781 aataaatgct cttatatgat tagatatttt tgcataatta aacgataaga aatcaaattt
    12841 aattttacat aaatagaata tatattttta tataataatt gaaaataaaa gaggatacct
    12901 cataatgata aaagaatcac tttgagatac cctcttacaa acaactaaat caataatttt
    12961 ctatctttca cactcttgat ttgcaactgt acaagaagtt ttacaagctg attgacaaga
    13021 agtttgacac tctccacatc caccttttgc agcactttgc tttaaagtag cttgagataa
    13081 agtttttaca tgtttatttt ccattttcaa cgcctccatt tctaattgtt tcctattgat
    13141 tatatcataa aactacttta atataaactc tatttaaagt tgacacccaa tacgcctcca
    13201 atgcctcctg ctgctgatat taacaataac ttataaatca tccctacttc aaatacaaac
    13261 ttttcttgtg ctaaaaacac agtcaaaaaa atacatacta tgtacaaaag tcctactaaa
    13321 agcccataca taagtccttt ttctttcatg tgttttgaag tgtaaaaccc tcctatagct
    13381 gcagaaatag tagttataaa tgatgagacc atagttatat tatctccacc aatatctgta
    13441 aatgtcaaaa ataagttata cactaataaa acagccaaag ttattatata tgcatatcct
    13501 agacctttaa aaatataatt agattttccc ataaaaaaac ctcctctcaa attaatatat
    13561 attcgagaaa aggcttttaa attactaatt tagtcttctt ctatttcttc ttttttagat
    13621 ttaacagttc ctatagccca tttttcaaat ggaactttag ttctatttgg tcctaattct
    13681 aatattacaa catcatcttc aactttagct acattagcta ctattccacc tattgtaatg
    13741 accttgtctc ctacacttag gctacttctc atttctttta attgtttgtc ttttttcttt
    13801 tgaggtctaa tcattaggaa ataaaagatt gcgattacta ctacccatag acctatactc
    13861 ataattattt gttgctgtgg cattaatttt tcactccttt gtaaattata ctcttttgta
    13921 aatcataata aatgtttata taacaattta ctttctctat ctttgagtac tattgaatat
    13981 tatatattaa aatgattata attaatagtg caaaacctat atttcatatc catattttgc
    14041 aaaaaattca tttctaaaat caagtaatct atcttccatt atcgcttgtc ttatttgttt
    14101 cattaaattt aataaaaagt gaagattatg agtagttatt aatcttgcac ccagtatttc
    14161 atttgctttt ataaggtgtc ttatataagc tcttgaatag tttttacaag cataacaatc
    14221 acattcaggg tcaagtggag taaagtcttc agcataagtt gcatttctaa ctactacttt
    14281 accttggcta gtcattgcag ttccatttct agcgatacga gtaggtagga cacaatcaaa
    14341 catgtctact cctcttataa caccttctac taagtcatct ggactaccaa ctcccattag
    14401 gtatcttggt ttatcctttg gcataagtgg agttgtatgc tctaacacgt catacataag
    14461 tggttttggt tctccaacac taagtccacc aatagcatat cctggtaaat caatattagt
    14521 tatttctttc gctgattgtt cccttaagtc tttatacatc ccaccttgta ttataccaaa
    14581 taaagcttgc ttgtcggtat tattatgggc atctttacat ctttttaacc atcttgttgt
    14641 tctttctaaa gaatttttta catattctct atctgatggg tatggtgcac actcatcaaa
    14701 tgccatcatt atgtctgaac ccaatgcatt ttgtatttcc atagcttttt ctggagttag
    14761 aaaatgtttt gaaccatcta agtgtgacct aaattctaca ccttcttctt ttatctttct
    14821 taaaggccct agactaaata cctggaaccc tccactatct gtaagtattg gtttatccca
    14881 attcataaac ttatgaagac caccagctct ttttataagt tcatgacctg gtctcatata
    14941 taaatggtat gtgttactaa gtattatttg tgaaccaatc tcttttaatt cttctggtgt
    15001 catagatttt acagttgctt gagttccaac tggcatgaat attggtgttt caataatccc
    15061 atgtggagtg tgtaatcttc ctaaccttgc accactctgc ttacaagtct ttatcaattc
    15121 gtatcttact gcgtacataa agttctcctt aatcatattt atttctcatt atttcttaag
    15181 atatatttgt aataagctat tcatataatt tattgtctaa atagatttat cttcgtcttc
    15241 tttgttctgt tgttccttaa ttgcttcttc atcatgaata ctcattatat taagagcact
    15301 cttgtctttt gttactaaaa acttttttat agcaaatagt cctgctatag ctataactcc
    15361 tattaaaaca tctatcatac cttcattttt aggcagtagc aacatttttc tagcaattgc
    15421 gtaaagtaat gcctcaataa atgctcctgg tatatgaaga gatagcatta ctacaagttc
    15481 aacacctatt actaaaagta atatttgtgc taaaaaatca ttaagctgac tgtattgtac
    15541 tggattttga aaatcaacta tatatgctcc ccacataagt cttagaacat ctatagttcc
    15601 taaaaatact gctattagaa ctactactgc taaaacacgt tcaaatataa aagcaatctt
    15661 taatataggg ttattctttt tttgtttttg taaactatgc atctattacc tccctacttt
    15721 ataatcatag catctccaaa actgaagaat ctatatcttt cttttactgc ctcattatat
    15781 gcatttaaaa cattatcctt tccacaaata gcacttacta acattattag tgtagactct
    15841 ggtaagtgaa aattagtaat aagtttatct aaaactttaa atttataacc tggatatata
    15901 aatatttctg tccatccact agtttctttc atctttccat cctcattaca agctgattct
    15961 atagttctac aactagtagt accaacacat attacatttt ttccattttc ctttgctcta
    16021 tttattttgt cagctgcttc ttggctaacc atataatatt ctgaatgcat cttgtgattt
    16081 aaaacatcct ctacttttac tggtctaaaa gttcctaagc caacatgtag agttacaaag
    16141 gctatatcta cacctttttc ttttatttta tttaacaatt cttctgtaaa atgtaatcct
    16201 gctgtaggtg ctgctgctga accattatgc tttgaataaa cagtctggta tctttctttt
    16261 tcatctagtc tagcagtgat atatggaggt agtggcatat ttccaagttc atccaatatt
    16321 tcttcaaaaa ttccatcata atggaatttt ataattcttg aaccttcatc tgataaatct
    16381 acaacttctc caattagctt tccatttcca aaagaaaatt tagtccctac ttttgctctt
    16441 tttcctggtt ttactaaagc ctgccaagta tcttcctctg ttctttttaa taataaaaac
    16501 tctattttac ctccagtttc aagcttctct cctattaatc tagctggtat aactctagta
    16561 ttatttaata ctaagcaatc tcctggattt aaatattcta ttatatctct aaatacttta
    16621 tgttcaatat tacctgtctc tttatctaat accatcaatc ttgagctagc tctatcttct
    16681 ataggaactt gagctataag ttcctgtggt aagtcaaatt taaaatcact tgttttcaat
    16741 gttaaacttc ctctcttaaa atttctccag ctacaataat atcaaaaaaa caacttattt
    16801 tccacaatat taccataatc tatcaagaaa ttgctatttt taacagacac catatccaca
    16861 atatatagat atttttccac gatatacatg ccttatccac aatatataga tacaatacac
    16921 acaattttta tcgtgtttat ccacagtatc tacagatata attaccattt tgtggatatg
    16981 tttgaatttt tagccatttt agaaatattt taatttaatg actcttttat tttataaata
    17041 ctcgtataat ctatttttat ttttcattgg gatatggaat ttttaagtgt tcatatgcta
    17101 atggcatagc tactcttcct ctaggacctc tatttataaa tcctaattga agcaaatatg
    17161 gctcatatac atcttctatt gtatttctat cttctccgat agaagctgcc aatgtatcta
    17221 aacccactgg tcctcctcta aatttttcta ttatagtcat aagaagcttc tcatctacaa
    17281 aatcgagacc caaactatcg actcctaaaa gttccagagc atcttttgcc actttatcag
    17341 ttatttttcc atcagctctg acttgtgcaa aatctctaac tctttttagt aatctatttg
    17401 ctatccttgg agtccctctt gaacgttttg caagttctaa tgctccatta gactgtatct
    17461 ctgcatctaa gatgcttgaa gacctaagta ctatttttga aagttcatca actgtataat
    17521 agtccaattt acaaataaca ccaaatctat ctctaagtgg atttgtaagc ataccagctc
    17581 ttgtagtagc tccaataagt gtaaatttag gaaggtcaag tcttatgctt ctagcagatg
    17641 gccctttccc tattattata tctaggcaaa aatcctccat tgctggatat aaaacttctt
    17701 caacacttct attgattcta tgaatctcat ctataaataa aacatcattt tcatttaaat
    17761 tcgtaagtat ggctgccaaa tctccagctc tttctattgc aggtcctgac gtaattctta
    17821 aatttacccc catctcattg gctattatac ttgctagtgt agtttttcca agacctggtg
    17881 gtccatataa aagcacatgg tctaactgct catttctcga tttagctgct tctataaata
    17941 tactaagttg ttctttggat ttttcttgac ccaaataatc ttctaaagtt tttggtctta
    18001 aactgttttc tatatctata tcttccatct tcattgtaga agttattatc ctattttcat
    18061 cttcaaaacc ttgcatttat acccctcctc tcgtactttt gaaaatctac tttatatatt
    18121 ttcaaattta cataaactcc ttaaatattg ttttaaaatc caaatattat aaaatccaaa
    18181 tactatttca aggatttact cattatatat gtaagtgctt tctttataat agcttctgta
    18241 ttcataccat cttttttaca tttttgaact gcctcttttg attccgacaa agtatagcct
    18301 aaagctacaa gtgcatctac agactcatct tgtgatatca aggtaggttt ttgtgataat
    18361 aacgttggtt caaactctat gttatttttg tctaccttat ccttaagctc taagacgatt
    18421 ctttctgctg tctttttacc aacaccagga gctttggata attttccaat gtcttcactt
    18481 aaaatatatg ctcctaactg tgctggtgat gcaaatgata gtattgaaag cgccacctta
    18541 ggtcctattt tagatacaga tgttagtaat tcaaacatct taagttcttc cctacttaca
    18601 aaaccacaaa gactcatatc atcttctctt acaattaatt tagtatatat ttttgtggct
    18661 tctcccactt gtaaattcat tattgtattg gatgatacat ttattttata tcctatccca
    18721 ttattttcaa caactatact gtctatataa atttcttcta ctgtaccttt tatataacta
    18781 tacatactta ccacctatat tctttagagt tttttcaagt ttgtttgaat gagcatggca
    18841 aatagccaca gctaaagcat ctgcaacatc atctggtttt ggaacttttt ttagacttaa
    18901 aaatgaagtt accatctgtt gaacttgtgc cttatctgct ctcccatatc caactacacc
    18961 ttgttttact tgaagtggag tgtattcata tacaggcttc ccattatgag cacatgcaag
    19021 catagtaaca cctctagctt gtgcaactgt tatagctgtt tttacattct tgttaaagaa
    19081 taattcttcc attccaactt catctatatt gtaattcttt actattgtat caattccttt
    19141 atacacaagt tccaatcttc tcgatatatt catatgggca ggtgttgtaa ctgctccata
    19201 atcgattgct ttaaacttgc tatttttgta ttcaattata ccatatccaa ctatggctat
    19261 acctgggtca atccccaata ttatcatttt ttaaaattcc ccctaaaaat aaaagaaata
    19321 ccaaaatgta tttctcataa aaacttatgt tctaaatcat tatatcataa ataatattaa
    19381 caaatcataa aatgctctat gtaaaatata aaatcaaata aaaaagttat atcaaagcaa
    19441 gattttacct caatataact tttatattta ttacaaataa attattgtat ttttatagga
    19501 attaaatgtg actacaattt taatactaat ctatttaata ttaaattatt tattattaat
    19561 ttacttagta tttaacttaa aataatatta atttactatt aacttgcaaa gtcttctaat
    19621 cttgaatagg cctcttcttc agtatccata actacacctt ttttgatttc atcttgaact
    19681 gttttaggaa gtgaatctac tcttattttt gttttttcta ctaatgtttt acttccgcta
    19741 tcatcatatt tatatagtgc tatatttcca ttatcatttt caatagtata tttacctttt
    19801 atagtaacat ccattgcttg tacatcttta ttatcactat atttagattc taccaatatg
    19861 atttcatttt tatccatact ctttattttt ttagttggat attctttttt aaaatatgat
    19921 actatttctg acttcgtctt atctaataat gactctggaa ctattcctgc cattgtaagt
    19981 gttctcttat agttagtttc attttcgcct tcactttgaa gccaaacttc acaattttta
    20041 tttaatccaa aagcttcttt tgacttttca ttcatattcg cactagtact tacaacttca
    20101 ccagtttgaa ctccattgtg ttttaatctt gtccctatat aaactccgct acttaataca
    20161 aatactccaa ccagtattaa ggcagatatt ttccaaggaa ataattcttt tttcttcttt
    20221 tcaaacattg attacgcctc ctttttctat tattttctcc aagctgtaag tttttataca
    20281 atttaggtaa attttagaag aaaatatatt tatatttcaa attactctaa gaatagatat
    20341 tttctaaaag gtagttttct cttaatggtt atattcttgt aattatatgg acataattta
    20401 taataaataa tcgtttagga ggtaataact ttggaaagaa taaactgtaa tgtaattaac
    20461 tgttcacata acaacagcca tatatgttat gcagataaaa tttttataaa tggtgaaaaa
    20521 gctcacaatg acagacatac ttgttgtagt tcatttttag atgaatctat ttatagtaat
    20581 ttatctagca acgttaataa taaaaattgt gaggaattag tttgtactgt aaaaaattgt
    20641 aaatacaaca aagatggtag cttatgtaca cttgataata tattggttgc tcctgaaact
    20701 gatagagtaa acctttatat tgaaacatgt tgctctagtt tcgaatgtaa atagtcatag
    20761 tataaatata agggtgtcaa atgtaattaa actaacatag tgtagagtat ctacaactat
    20821 tagtttgatt atacttgaca cccttattta ttttaaataa aatttcatta ttataaccat
    20881 gtttataatg ttctaactgt aattaaattt gataaatttc tataaattgg tataatcaat
    20941 atgtaaattg aatttagtaa attaatcata aaaataaaat atcgagtata tagtgttata
    21001 ctaactagaa atgaggtgct tatgagtaca ttttatattc aatttcttta ttttgtaatt
    21061 tatagcttta taggatggtg ttgtgaaact acatattgtt cagttttaca aaaacaattt
    21121 gtaaatagag gttttttaaa tggtccattt tgccctatat atggatttgg agcattagca
    21181 atagttgcta ccttgactcc ttttgtacac aatattcctt tgttattttt gtttagtata
    21241 attataacta gcataatgga gtactgtacg agctttattt tagaaaaaat atttaatatg
    21301 acttggtggg attattctaa acataaattt aatattcatg gaagagtttg tctggaaaac
    21361 tcacttatgt ttggtgtatt atcattgatt gtaatgctta tagtacatcc tattgtggtt
    21421 gactttatta actctatctc taaaaatgtg ctatttatat ttgctatatc tatagaaatt
    21481 tattttgttt tagatttagt tatcacagtc catacaattt tacaattaaa tggtaagtta
    21541 aaacaaatca atcttataat taaggaatta aaagataaga aagaatacta caaacttatt
    21601 acacaagaaa ctatagaaaa caaacttgaa tctttagttg aaaatagact ggatactttg
    21661 gaagataggt ttgaaatact taaagaaaaa gaaaattatc gctatgctaa aaatcgtatt
    21721 gatgagctaa aaaataaact taatactttg ctgtctaacc acaaattact tcatagacgt
    21781 ataatcaaag cattccccaa cataagctct aataagcata cagatatttt aaataaaatc
    21841 aaagaaaata ttaaatcaat taaaaaagat aaataataaa ataaacacat aagttaaata
    21901 ataaaaatac taatacataa gattaaggtt tagaatattt tttgtaaaat actctaaacc
    21961 ctaattaata tactattatt tttaatctta atttataatt ttagtttatt ttaattttaa
    22021 ctcagtaaat ttaagcgttt aaacttcaat acactaatat actaccccat caatatctca
    22081 actgattctc taagtgtgct aatctctccc acatttccag ggaaaattat atatggcata
    22141 ttaggaaatt tgctttcttt gcctgtcatc catactggta taccaggttt tatctgtccc
    22201 attactgtag ctttttttac tcttagtgct ttagtaccaa catcactaga agtaattcca
    22261 cccttagcta taataaaatt tggtcttaca ttaagcatac caattatact ggtaactgca
    22321 tctgatattt ctacagaaat cataagctgt ttatctttat cattcgtatc caagtcaaaa
    22381 cgctctcgtc tagtataaac agcaacagtt ttaccactgc ttattttttc ctctacaact
    22441 tttataactc tttttacctc attttttaga ccattttctt gaagaactaa atgttgatta
    22501 aattctataa attctatcgg atatttacaa tttttaagtt cttctaactg ttgggtagtc
    22561 ttattcacat gtgaaccaac aagtataatt cctccattgg tattaccttt tgatactaat
    22621 tcatccttag taagtagttc cttgtcactc actccgccaa gtacttttgt tatggcagca
    22681 gcgcttctaa acataaattc cttaccacca tttactgctc gtataaacgc aattgtaaat
    22741 acctttacat ctacataatc ggttgcattt acaataattt tattgaatcc ttcagctttt
    22801 ttaagttttt ctgttatagc ttcaatattt agctctctaa gttcttttga cgaaatatat
    22861 atcatatcac tagatttgta ctttccattt gtcttttctt cacaccactc acctatatca
    22921 gatgatttat atccaaaaga tttatctttt gcaaattcag tcattcctgc tggtacaagt
    22981 atttctcctt ctttaacata atgtacatta tctatagtaa acctaccacc ttctttaaaa
    23041 aatggcatta ttatttcacc ctgaaataac ttccccgata atctctctac ttcgcttttt
    23101 aataaattag tttcaagagg ataatgtcct ctcatagtag aatcactacg acttatgatt
    23161 ataaaatcct tattaacttt ctttgataca tccacaatat tctttgaaat ttctttatgt
    23221 gcttttgtag tttgagcaac agtaaatccc cttgaattgg taagtataaa aaacatacta
    23281 tttttctcat taaagccttg ttccatacta tccttatccc aatcagtata aacagaaata
    23341 tcatgaactg tttgtacacc tgttgggtca tcatctaata caacaatttt tctatctaaa
    23401 tgcttaagct catctgcaag aagtttattt aatctatcct catctacttg tttaaaagta
    23461 ttaagtatat ctatgctaat tggttttact tctctcattt aaaacgcccc tttatgtaat
    23521 ttgtatagtc taaatataat ttatatgata taaacaatag tttgttattt atagacctgg
    23581 aagtggtata attccattta taacactaag caataaaacc attataaatg ctacaactcc
    23641 agcaatggtt cctccaattg tccaagagtt taaagtatct ttaactgata atccactaaa
    23701 tcttgaaagt acccagaatc cagaatcatt tggaagggag aaaccaactc ctccagcaca
    23761 tatagcaagc cctactaaaa ctggtgacat tccagttgcc aaaattgtag gtcctaaaat
    23821 tgatgatgtt gttactaagg ctacagtagc agaaccttga gaaattctaa gcaaggcact
    23881 taataagaat ccaagtacta caactgatat actaagacca gacatagtat caactaagaa
    23941 gttacctata ccacttgcat ttataacact gccaaacgca ccacctgacc ctgttatcaa
    24001 taatattagc cctgctgaat cagcagcttc aataactaca tctcccatag gtttatttat
    24061 gtatttcttt aaaaatccta ttgccaccaa tattccaaga agtattgcta tgtttttatc
    24121 tcctataaat gcaagtactc cactcattgt agaacctttt tctaagaaca tactaaatac
    24181 atttgatata agtataagaa gtattgggaa caataaagaa aacatagaaa ttccaaaacc
    24241 tggtacttta gaagaatcat catcttcaat ttttatgcta tcttttccct gctctaaatt
    24301 tttttctcta tcattctcat caatagcata agctggatat ttttcaaaac gttttccata
    24361 aatccatcca cctataagag ctgctggaaa agcaacaatt atagaataaa gtataaaact
    24421 acctacattt gcatttacat ttgctgcaac tgccaaaggt cctggtgtag gtattacaag
    24481 tgcatgacct actatagtcc caactcctaa ggcacataca aaaaccatta gagatttctt
    24541 agttactctc gaaacatact taattatagg catcattata acaaaagctg catccataaa
    24601 aactggaatt gaaattaaaa aacctgctgt agttactgct aaagcactgt ttttttctcc
    24661 ggtcttagct aacaacccct ttgcaataga ttctgttgct cttgattctg aaagaatatt
    24721 tccaaatatt attccaagtc caattacaat acctatacta cctaatgtat ttccaaatcc
    24781 agtcgatatt gtggttgata tttcatttat aggcattcta accataaagc ctgtaattat
    24841 agatgttatc agtaatgcca aaaacggatt tactttaaat ttaataatcg atacaagtaa
    24901 aattcctata gatataacaa atgctatcaa aagtccatat cctgtaatca taatctttac
    24961 ccctttatca ttctacttta ttatttatca attttatttt tcttttacta tgataactta
    25021 acctcgctgt atttatataa tctaatccta ataatatgta tttaattata ttgatatatt
    25081 taaatattaa ttatcgtgtt tttttatttc aacacctgcc aacttttcaa aatactgtgc
    25141 tattccacta tggtcatctt ccaaatgtcc atgtactttt aatgtctgca taatttctaa
    25201 taattgactt gtcataggta gtggtacatc taaattgtgt gctgtttgca ttacattctt
    25261 aatatctttc aaattaatag atatttttcc acctggttta aaatcacgat tcataatcat
    25321 tggtatcttc acatccaata ttgtactacc agctaggcct cctcttattg ccttatatac
    25381 tttttctggg tctgcacctg cttttgctgc aagtacaaat gcctcagaaa ctgctgctat
    25441 cgtcaaatta actattactt ggtttgtaag ttttgtaaca cttccactac cattatctcc
    25501 aattaaaata gatgagcttc ccattatatt aaaaaatggc tctactttct tataaatttc
    25561 ttccttacca ccagccataa aagcaagtgt accatcaatc gcttttggct cccctccact
    25621 tactggtgag tctataaatt ctaagcccat atcttttaat ttatttgcac acaatattga
    25681 ttctgtagga gtaactgaac tcatatcgac tactatactt ccttctttta aagtttttgc
    25741 caatccatcc atcccaaaca atatatcctg cacaatagtt ccatttggaa ggattgtaaa
    25801 tacaatatca cattcttgac ccatttctaa aacacttgcg tattttgctc cacatgatat
    25861 aagttcatcc acagcagatt tattaatgtc atacaccaaa agattacatc catcttttaa
    25921 taaatttttt gccattggct ttcccattat ccctaatcca ataaatccca atttcatttt
    25981 ataaccctcc atatcttcac atttttgaaa acgtttttta tgttttagag tatactacat
    26041 accagtatac cggtcaatat aaaatataaa agtatttatt ttaacaataa atagtaattt
    26101 ttaaagtcat ttattatatt tagtcatcct atataagttt taatacatac tattgttttc
    26161 aaatataata tactactttt acagttttgt attttaacta atttcaatta gttttaatta
    26221 atctgccttt aatgttaatc atgcttcaca cttcaataat tttttatatt ttttaatctt
    26281 atctgcaatt taaattgcaa aagattgacc ttattttcac tatgttgtat gcttaaaatt
    26341 atataagttt ttgtattttt atattgttta ggggggtatt attaagtatg gctacattta
    26401 aaagattaca agaacaagca tatgactact taaaagaatt gatactatct ggagaaatga
    26461 tagaaaatga aatatattct gaaacaaaac ttgctagtga aataggtatc tctagaacac
    26521 ctataagaga tgctcttcaa agacttagtc aagatggatt tatagatata atcccaagca
    26581 aaggatttag aatccatcaa attacagcaa atgaaattgt agagatattc caaatacgct
    26641 ctgcaataga aggattttgt acttttttaa ttacaagtca atacaaagaa gctagagcag
    26701 tagaaactat aagtaaatta aaacatcttt tagataaaca aaaagatatt ttattaggtg
    26761 ataaaaattt aaactcattt gcagaatacg atacattatt tcacacaact atagtttcat
    26821 atgctcaaaa tactgaattt gacaaaatgt ttaacaatta tatgtacaga ataaaaaaat
    26881 tagctctcga ttcattgtca catgaaggaa ggcttgaaac aacattaaaa gagcacacag
    26941 atatttttaa taatattgca aatggatgta ttgaagatat ttacagaact actctcattc
    27001 atatggaaac tcctaaatac attaatttag aagatttttg taacaataag ttattttagt
    27061 attatacttt catttattaa aaatatatct atctcaaatt aatttcctaa tttataaaaa
    27121 atattctata attatattgt aactaatact tattctaagg agtgattttt ttgaacgaag
    27181 atgttgtaac taatgaaact attgacaaag attatgctat tgaagaagtt cgtatggcat
    27241 gcaaacattt tggtgattta tatttttatt tttccaaagt gttatttgaa gagtttggtg
    27301 aagataaaac tagcgaaata ttaagaaaag ttttatttga aaggtctgaa gaaagagcta
    27361 ttgctatgag agagagagct catgaaaatg gagatgaatt aatcgcagac aatattatta
    27421 gcacaactga tgttccattt ctaggatggg ttcctgaatt tgaagagtta cattgcccct
    27481 atggagcttc ttggctttta agatttgagg aaaatccttg gtttaaaaaa tttgcttctc
    27541 tttactgtga tgttacagat actactgtag ctgaagtatt tacaggagat acgtcccata
    27601 aaataacaaa aaacattcta tggggagata aaagttgtga aagaatatat ttccatgatg
    27661 acaaggtatc tgaaggtaaa tatacttatg gaaataaaaa ctgtaaataa atgatttaaa
    27721 ttttctatat tcatatacta tattttgtac tttttcatat aaatattata actagtcttt
    27781 tattttactt ttgtttaacc taaagataat ttacttttaa tctatatgaa ttatcatata
    27841 aataatcaga aagttatctt tataaattat agttttagat ttataattta taaaatattt
    27901 atgttctttg taaaattttt tagaagcttc taatgttatg ctataaaact atcttgagaa
    27961 gggctgattg aatgtttaat aaaatatatg aaaagaaaga atttatagtt tttcaagtta
    28021 aaaaaggtta tgtagtttat aatactagaa agagttttga agaaggtcat acacatttaa
    28081 agcattttga agcagcaaag acagctattg atttagcaat aaataaaaag attcctagaa
    28141 gcaaagatgg atattatctg actagtttaa ttcgcataag tgatgatggt tattacattg
    28201 ataagttaag cgaattgtta tatgttagag aacaaaaagg taaaaaagaa aagtactgta
    28261 attctggtta tcagatgtaa caagactagt tgtgaattat aagtaccaat cataagtacc
    28321 tttagtctta aaatcaaaga gtatcagtaa atctataaaa aaacaactta ctgatactct
    28381 ttttaattat atgattaata tttgaatttg atacaaaatt ttatacaaaa gtttatgtta
    28441 tctattctga ttctttgtct gcaaattggc tattataaag cttctcataa aatccaccct
    28501 tttccattaa ttcatcatga ttcccctgct ctataatact tccattattc ataactaaaa
    28561 tcaagtcagc attgcgaatt gtagaaagtc tatgtgctat aacaaaacta gttctaccat
    28621 tcataagatt tctcattgct ttttgaagca taagttccag cctagtatca acagaactcg
    28681 tagcttcatc aagtatcaat attgaagggt ctgatataaa agctctagct atggttaaaa
    28741 gctgtttttc ccctagagat atattagatg cttcttcatt taaaaacata ttgtatccat
    28801 ctggaagagt ggttataaag tgatgaacat ttgctacttt tgctgcttgt ataatttctt
    28861 ctttagtagc cccaaatcta ccatattcta tattttcaaa aatggttcca ttaaataacc
    28921 atgtatcttg aagcaccata ccaaacattg aacgcaaatc tttacgtttc atatctctta
    28981 tatctactcc atcaatttta atagcacctt tctttacatc ataaaacctc atcaataagt
    29041 ttattaatgt tgttttacct gctccagtag gtccaacaat agctaccatc tgaccacttt
    29101 ttacttctgc atttaaatct tctattaaag ttgaattttc tccatatcca aaattaacat
    29161 gctcaaatgt aacatttcct tttacatttt ctatcttaac tggattttca acatcaggta
    29221 tttcctcttc ttcgtctaaa atttcaaata cacgttctat agctgcaaaa gaagattgta
    29281 ttactgtaga taactgagtc atttgtgaaa gtggctgatt tatctgccat atataacgca
    29341 caaatgcttg taaatttcct actgttattc ctccacttac tgcatatata gaaccgacaa
    29401 ctgccacagc tgcaattcca aagtaagtta ctaatgaaac cataggattc atcatacttg
    29461 atataaattg tgccttaaat ccattttcac aaagttcttg atttactttt ttaaattctt
    29521 ttatagaatc ttcttcttta ccatatagtt ttatttcatt aaatccagta tacatttctt
    29581 gtacttttcc atttaatttt ccaagagcat tttgttgcaa tgaaaatcta ctttgagatt
    29641 tttttactac aaatttagat acaaaataac taattggaat tataaaaaga gctataagag
    29701 ccatatatat attaattgta aacatcattg aaagtgccaa aattatgact aagattgagt
    29761 ttacaatttg cataaaactc tgctgcaatg catttgatat tgtatctaca tcatttgata
    29821 tacgacttaa tacatctcca aaactattac tatcaaaata actaataggc aatcttctta
    29881 ttttattttc gacttcattt cttaaatctc tcattgtgtt ttgaatagca ttcgtaagta
    29941 gaaaacttga agtatacgta aaaattgcat tggcaaaata tatacatgct aaaatcacca
    30001 atatttttgt tacataatca aaatttacgg aagcccctgg tactcctttt gcgatactaa
    30061 ttacatcttt agtcaattgt gttattatca atccttcagt tttaggtgca agtgcattca
    30121 tagtagctgc tgaaattata catataatag ctataataaa ggaaaattta tatttcttta
    30181 caaatggaag aagtctacca attgttttct taaaaccttt agactttttc attattctaa
    30241 ttcctccttt tttaactgag atgttgcaat ttcatagtat atttcacagt tttttaataa
    30301 ttctttatga gttcccattc ctacaacttg tccttcattg agtactataa tcttatcagc
    30361 atcaataata gaactaattc tctgggcaac aatcaataca attgcatctt ttgtttcctt
    30421 cttcaattta gcacgaagta ttgcatcagt tttaaagtca agtgcagaaa aactatcatc
    30481 aaaaatgtat atttcaggtc ttcttataat agcccttgca atagaaagtc tttgtttttg
    30541 acctcctgat acatttgctc caccttcact aatcaattca tcaaactttc taggtttttt
    30601 tgatataaac tcatatgctt gtgcaacttt tgcggaatat tctaattctg actctcctgc
    30661 cttatgtttc ccaaatctaa tattatttgc tatgctacca gtaaaaagca gtgatttttg
    30721 aggtatgaat cctatctttt ttcttaaagc ttttaaatca tattctctta tatctactcc
    30781 atttatctta atgctacctt ctgttacatc ataaaatcta ggtatcaagt taattagagt
    30841 acttttccca ctaccagtac ttcctataaa agcgactgtt tcacctgttt ttgcagtaaa
    30901 tgaaatatcc ttaagtacag aagcttctcc atctggatag gtaaaagtta cattatcaaa
    30961 ttctatgata cccttatttt cagtatcctt aattccattt tcaggatttt ttatcaatgg
    31021 ttcttcattt aatagttctt ttattctatt tgcagatacc tctgctcttg gatacataat
    31081 aaataccatt gaaaaaagca taattgaaaa catggcatgg aaaagatatt ccaaaaatgc
    31141 aaccaactgt ccaacttgaa gctctccaat atttatcttc tcacttgaaa tccaaaatac
    31201 agccaaaaca gctatattta atagtaaaaa gaatgctggt tgagtaattg acataagctt
    31261 aaatagtttc ttagaaacat tagcataata tgtatttgtt ttttcaaatc gctctgtttc
    31321 ataatcatca tttccaaaag ctctaattac tcttattcca gttaaatttt ctctcgatat
    31381 aaggtttaat ttatctagtc ctttttgttg ttttgaagat attgcctgag atacttttgc
    31441 tattattgct actccaataa ttataaaagg tactgatata gccaatacta tagaaagttc
    31501 cacacttgtt ctaattgtca ttattatact tataatgaac ataactggag ttaataaggc
    31561 agtccttagt agtatattaa caaattgttg aatttgaaat gcatcattcg ttgttcttgt
    31621 aatcattgaa gaaattccaa atttattgta ttcagtatgt gaaaattctt gtgatttctc
    31681 aaatatgtca tttcttatat ctctggttac acttgttgat accttagcag aacaataacc
    31741 caacaatata ctacctacaa cccctataat agaaataata acaatcacta ttcccatttg
    31801 ttttatgtaa gctatattct gatttgcaat accattgtca attattttag ctattatagt
    31861 tggaatcccc aactcgacta atgcaaaact aaatatagcc agtatgttta atactattaa
    31921 taacttataa ttctttaaat aacttaaaat taatttcata tttttctcct ttcttaattt
    31981 ttgagatagt gtaatcatgt aataatacta aatttataaa tgcttaattt tataaaaaaa
    32041 ggagaaaaga gttaacgcac tctcttctcc agcatattct ttttaataca ccctccgaca
    32101 agtcgcttat atgttaacat atttgacaag taatgtcaat tcatatagcc aaaagtcttt
    32161 taaatgcttc gtttcataac attgtaacaa attggcatgt aatttatttt tgttttttac
    32221 tgtattccaa ttttacaagt tctccaatat tactagcttt cttaaaattc aaattagtct
    32281 tatctatacc agacttaaat agtgaaatcc ctctccccaa tattattgtc aatatatata
    32341 tataataatc atcaattaag ttatctttca taaataattt tactatatcg cctccaccaa
    32401 ctatccaaat atctttttta tccttctctt ttccaatttg ctcaattaat tttttaggtt
    32461 ctaaattaat ttatagtgtg attattctat gtaaacatca cttttttact cattcaataa
    32521 taccctatta ataaatcctc aaactaattt aaataaactt tgtataaaat taaaaatata
    32581 ttatataaat aatttaaaac tacactaact tattgaaaat tctaattatt acaaataaat
    32641 attgctctct gttaacgcta gttctataat ataattataa gctattaata ttataaagcc
    32701 gatattacta atataataat tataatactt atagttaatt tacttttgtt gaaaggtggt
    32761 aaataacatg aaaatttcag aagtcgctac tccaaatttt ttgctagatt tagaccagtt
    32821 agaaaagaat atagataaaa ttcaacaaat ttgtacaaat aataataaac aactttggcc
    32881 tatgcttaag acacataaaa gtacttacat agcgagactt caaaaaaatg caggtgctag
    32941 tggttttcta gttggaacac ttgatgaagg tgaagctctg ataaaagcag gttttgaaga
    33001 tattatgttg gcttatcctt acatgggaga agtaaatata aatagaattg tgactatggc
    33061 atcaaaaata aatttaatct gtgctacaga taatatagaa tgtgctaagg cttatagtga
    33121 agctttttct aaatcaaaaa tagagtgcaa gctacttatt attgtagatt gtggcttaca
    33181 ccgttttggg gttgaaccag aaaaagtggt tgaacttgct actgaaattt ctaagctaga
    33241 aaacatcaag attgttggta taagttcgca tccaggtcaa gtgtatggtt gtagttctcc
    33301 agaaggtgtt ccagatgttt gtgttcaaga agattcttct atggagaaag catacaaagc
    33361 tctaactgat aatggattta atatagaaat tgtcgcttct ggtagtactc caacagttga
    33421 atctgaagct gcttctaaaa ctataactgc tgtaagacct ggaaactatg tatactatga
    33481 tgctatacag ataggtttag gttgtgctac tgaagatatg tgtgctctta ctgttgtgtg
    33541 cactgtaatc tcaaaaaata gcagtggata ctatttaatt gattgtggaa gtaagtgcct
    33601 tggacttgat aaaggtgcac atggaaatag tagtattgtt ggttatggta gggtatgtgg
    33661 acatccagaa cttactattg attcactttc agaagaagtt ggaaaaatta aagtaaatgg
    33721 tgaaactgat ataaaaattg gtgatagaat aagaattata cctaatcatt cttgttccac
    33781 tgcaaacttg acaagtaatt tattaggttt tagaggagat acaatagaaa aagtcataga
    33841 agtagatatt cgtgaaaatt caaaacaaat catcctttaa aaacacattt attctgatat
    33901 taacatgaaa gtatattttt cttaaaatta ttgctaataa ctaatgttaa tgttggataa
    33961 ataaaaaaca taaattctag aaatataaat ttaaaagaag aggctgtctc aaaatagaga
    34021 taagcctctc tttcattgtt gaaattatga aaatatactt gatttcatac aaaaagagta
    34081 tctcatgaac taaaaagttc actttgagat actctctttt tttaattaat atacacatta
    34141 attactatct tagtttatta ctatatatca attactactc attaatcctt gtatatatta
    34201 attcttatat atcatttata gttaggttat aattttatgt tatttacatt aatcccataa
    34261 cagtgagtaa aactcttact atcaacatcc ccactattgc atcaaatata ggaactgcca
    34321 acagtaacat tctatactta gtactggcat ttgctacaag cactattctg gcataatgac
    34381 caactagagt acccattaac atacatggca taattaatat ggttgcttgt ggtgcagtga
    34441 ttagaccttg tgcaaataag tttgcagctg tagctgctcc tgctgcctta gcaaagaatg
    34501 ctgctatcaa aactacaaca gattccccag gtagtccaaa aatacccatt actggtccaa
    34561 aaatatttcc taaaatatca actaaaccag ttaattgtaa aaactgcact attacataac
    34621 ccattatcat ggctggtaat atctgttcaa cacctatgta aaaacctttt tttgctcccc
    34681 ccataaatat ctcaacagca gttttcttag gtttactatt tacaattaca tttccagtat
    34741 tttgttgact catactacac agcctcctta gccttattct ttttatttct tacacctatg
    34801 attaatctaa ctagattagc accaagtatt ttacagaata tctctataag tattattgga
    34861 cctaatgcca ataatgctat tggtaaaagt ggagcttgag tatttattgt gttcagtata
    34921 acagctgaac cagcatattg atatgctaca aatatagttc tttcatcatc tgttatttcc
    34981 ccactttcaa ataattcttt cgtcattatt gaggctacat ctgaactagt aaatgaagat
    35041 acaaatgcta ttcctgctat tccaggtata ccaagcagag gtctaagcaa tggattaaat
    35101 atctttgatg ccgctttcat tcctcccata gattcaacta catctataag acctacagca
    35161 aaacagacag ttggaatcaa agtcagagca aataagaaac catcttttgc tcccgttcct
    35221 cctttaccca taaagtctac accatctgca atagtaccaa atgaacctgt cagattagca
    35281 aaatctaatg ctttaaggaa gccttgacta tctttaaaca ctcctgaaaa taatactatc
    35341 aatacaaata aagataaata ccctttccat gttaccttat tatctccagc tgttacatcc
    35401 atataaaatc cccctttata cctcaaattt atctaagttt aaaataactg tagaaattaa
    35461 tttcaccctc tcaaacatag aatctactac tgcatattct cttgatgtgt ggttccattc
    35521 tcctcttaca ccaaatgaac agattgtagg aacgtttgca atagttaaat aacttgcatc
    35581 tgaactacct ccaagtctct tagcatttaa ttctccaaat ccattttctt ttgacacttc
    35641 atttacaaat gtatgaaatc tcataacatc ttctgtagtt tcaaatggca tcatttcact
    35701 tataaaatta acttctgtag aagttccttc tatgtaagtt tctttacata tctctctaac
    35761 ttgttcttta acattctcca tttcttctac tttttcaaat ctaaggtcta cttttatact
    35821 acaatcttct ggaatagaat tttccactct tccaccctta atcacactta cacttactgt
    35881 agttccaaca tctaaattag tcaatttttg tatgcgtagt atcttattag ccatttcttc
    35941 tatagcattt cttccacctt caaaatcatt acctgcgtgt gtttctacac cttttacatg
    36001 tatattacat cctattcttc cttttctacc tacacacaaa ctattatcta ctaatccagt
    36061 ttccatatta aaagcacaaa gaccaccttt tgcttccctc aatataacat ctgcacctgt
    36121 tgaatcctta tgtcctatct cttcatctcc agaaaatact atttttattg gtctttcttt
    36181 gtatcctatt ttatttaatg ctttaatagc atataaggaa attattatac cacccttcat
    36241 atctaaaaca cctgggccat atgcttttcc ttctattatt ttaaatggat tctctccaaa
    36301 tgtacctgtt tcaaagacag tatccatgtg acctgaaaaa attattggct ttttatctat
    36361 attggaacca agtgtaccta tcaatgtact accattatct ccaacatcta ctaactcgca
    36421 atctaaacct tctttttcaa actcaaactt taatctctta gccaatttat ttacgctttc
    36481 tttacaatgt gtatagcttt ccatattaac aatctctttc cataatgaaa ctatttcttc
    36541 tctattctca tcaacaaact tagaaatttc atttaaatat gtattcataa tcttctcctt
    36601 tattttcaaa taaaatgtat ttctttccta aaatctaagt ttattttagg aaatcttaaa
    36661 agactagtcc ttaattacaa aatagcacaa ttattcttga cagtcaacat atttttcagt
    36721 cttttttata ttttctgatt tttctttcaa ttttcctttt gtaaaataaa ttgacgcgtt
    36781 ttatctacta ttataaaatg aggttataaa ttgattgatt agttacaata ctctcgaaat
    36841 tttaaaggag gttttatgaa atataaaatt ggttttttga caacatctta ttttatgtca
    36901 gataagttta aacaagctct taaagaatta aatgatactt gtgaaattac atttattgag
    36961 cttaaagggg agaatccttt taataattta cctaatatat actatgagca agtagataat
    37021 tttgatggat ttgtatgcag tggtatagtt ccatatagtg aacttataat aaatataaaa
    37081 gacataaaag ttccattaaa ttttttaaag cttgatgaaa aagattttta taaatactta
    37141 ttcaaattat taaatagcca aaaagatatt gactttacaa aatcatttat ggatttttta
    37201 agagaggaca acaattatta tgatatatat tcattaatag actctaataa atgtccatat
    37261 acaattaaag attttaatat ccctcaacct gtatatgact ttaaaaaatt aaatgataaa
    37321 ttattggaaa tacatttaga cttattgcga gataataaga ttaacctttc ttttactaga
    37381 aattacatca ttagttgtga acttaataaa cttggatatg attctattta catggttcca
    37441 tctacagaat caatcctgaa tacatttaaa tctctggtga atgaaataac attacaaaac
    37501 ttagacaaaa ataaatctgc aacttgtata gttacagtaa atagttcaga gtacataaat
    37561 gaagatttga tatttaaaaa tgatgagata caaaatcaaa tatataacac tatattaaat
    37621 tctctatcaa gacatggatt ctatggagtt caggtcaaaa aaaatgatat gaaaatagag
    37681 atacacacta ctaaagaaat gttaaacaat atgaccaata actatacaga tttttttatt
    37741 tcagaagatt taaaagaaat taaatatagc ttaaatatag gttggggcat tggaaataca
    37801 aatattcatg cagagcaaaa tgctcgtcaa gctaatggta aatctgcttc tttgaatgga
    37861 aactgtactt ttatagtaaa tgatactaat gatacaatag gccctttata ttcaaataaa
    37921 aattctaata atattgaaga tagctcaata gccaataaag ttgcttcttt tatacctcta
    37981 tctaatacaa atgtatctaa aatcatgtgt atgattaatg atagaaattc aaataatgtg
    38041 tcagcagaaa tattagcaga ctatatgaac ataactctta gaagtgctaa tagaatattg
    38101 agtatcttat ataaagcagg aattgcaact attgtaaata caaaactcga taatcaaaga
    38161 ggaaggccaa aaaaaatata taaagttgat ttcttatctt ttctaaataa gatgcagaaa
    38221 ttaaattctt aatatttaat tttttaataa aagcaatgta ttttctctaa ttttaaagaa
    38281 tcaaaatgtt taaattttat tagaatatac aactttttac tttaaaatac ctaaaccttt
    38341 tatcagtgaa ataaaccata atatatcgta acttattaga aaaacaaaat aatttcatat
    38401 acaaataata aaatagccac tacaatccat aatttgcagt ggctactttt tacactatca
    38461 cttattactt atttttaaaa attaattgta ttttttaaat aatttataca cacctattat
    38521 cagtaaagca ataaatacaa gtatatctat aaaaataatt attgtaacat gtctgacaat
    38581 attgtaaata aaaaatatag agaatatttc tatagacaac gctacaaaac acataaaatt
    38641 cgttaaataa tctgcaatag tatcactata aaaagcatgt atttgtaacc atctccatat
    38701 aaatggacgt aaaagtattc tgaatacaac acaaatgaca gggtaggcat aaataaattt
    38761 tttatctaca taattagcta caccattatt ccattggata ggaatttgag gtggcaactt
    38821 ttgaaatact gttattgcaa tttaaacact acctctctat tctattaatt tataactata
    38881 atatagtctt aaatattttc actctataaa attgtatatt tagtatatct taaaatcaaa
    38941 ctaattatct tttataagtt ttcatacaat aatatc
//
LOCUS       DAMJVK010000031        38772 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.031, whole genome shotgun sequence.
ACCESSION   DAMJVK010000031 DAMJVK010000000
VERSION     DAMJVK010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 38772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38772
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.031"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            <1..466
                     /locus_tag="PC414_001651"
     CDS             <1..466
                     /locus_tag="PC414_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDF2464048.1"
                     /translation="NNIKNILKENHITFEEYDHEPILSFEKAKEIREKLQYTGVESKS
                     LFIKDRSGNYYIFVTTEGKKLDPKTIKNVLGGKVSICSKEELTQKTQCLPGCVSPFGY
                     EKNISLVIDQDIFNHDKIIFSPGIPEKTFIITASGFKKVINNINNTTYKYIY"
     gene            complement(1175..2329)
                     /gene="nagA"
                     /locus_tag="PC414_001652"
     CDS             complement(1175..2329)
                     /gene="nagA"
                     /locus_tag="PC414_001652"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDF2464049.1"
                     /translation="MKSFIYADKFFMKHGIKEKGYLSIINGKFGEFQQERPNVTDNVI
                     DFSGKYIAPGLVDTHIHGLLGADVMDNTFEAINTISKGLLKYGVTSFLPTTLTDSVDT
                     LSDSVENISNSFNRVEGAKIQGIYLEGPFFTEKYKGAQNEKYFKDPDINILKLWQKKS
                     KNLIRKIAIAPERKGSIELTRYATKNNISVSLGHSSATFEQVKQVVNSGAKVFVHTYN
                     GMSPLNHREPGMVGAAMVLKDTYAELICDGHHVSPIAVKIMMDAKSSNNIALITDCMR
                     AGAMEDGKYTLGKFNVNVKNSVARLNSGSLAGSVLTMDKAVRNIVDWNISTLEDAIKM
                     ATYVPALSCNIDNVCGSIYQGMDADFIVTDNDFNIHETYINGICKYKVNK"
     gene            complement(2412..3137)
                     /locus_tag="PC414_001653"
     CDS             complement(2412..3137)
                     /locus_tag="PC414_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2464050.1"
                     /translation="MIKRNDKRPIYDQLVEILRTKIENEMEPNDRMLSERKICDEYGV
                     SRTTVRLAMAELEHMGYIYKRHGKGTFVAALSQNSQNLMESYSFTDYMRTQGKKPGTK
                     VLSFEVVESTKYFSEKLGITPGEKMIKIMRLRLADDLPMMLERTYLPMKEFIGLTKEK
                     IEQKPLYEIFREDYGEIIKVADEEFSAGIISDKEAKLLEVPGDSPCLKLLRTTYNDDN
                     IVIEFTLSVARSDKFVYRVRHVR"
     gene            3406..4335
                     /gene="pfkB"
                     /locus_tag="PC414_001654"
     CDS             3406..4335
                     /gene="pfkB"
                     /locus_tag="PC414_001654"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDF2464051.1"
                     /translation="MITVITFNPSIDRMYRVNNINIGEVQRVVSKNATAGGKGLNVAK
                     VCKILKENPLAMGFLGGFNGEFIKNELRKLDIQNKFTKIEQETRNCLNIIEDNKVSTE
                     FLEKGPIVEKSDLERFENDIEEVLKNTKILVASGSYCENMPIDYYEKIGNICRANNIK
                     FILDTSGEALKIALKSKPYLIKPNIDEIRHLLGMNIESREEIILSGKKLIEMGAENVC
                     ISLGKDGMVYLNEDNVYDVKVPVVKCINTVGSGDSTVAGFSVGILRGYKIENLLKFAN
                     ACGISNALNMETGFVDLGEIDKYQDLVEVKKLS"
     gene            complement(4464..5381)
                     /locus_tag="PC414_001655"
     CDS             complement(4464..5381)
                     /locus_tag="PC414_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDF2464052.1"
                     /translation="MKNIKMGMIGLGSIAQKAYLPILTKSERFEFVGAFTPNKVKREK
                     VCSDYRIMPFDSIESLAKKCDCIFLHSSTETHYEIIKILLNLGVHVYVDKPLASTVSQ
                     GEELIELSTKKNLNLMVGFNRRFCPMYKEIKNNATEIVSINICKHGLNSLRNVRFDST
                     LIDDYIHVIDTALWLANEDVEISGEDLFLTDNKNLIFVSHKLKGKNFSINTSMHRDSG
                     TKLEQVEILSKGKIQRVKNLNVLEIEEGGNLTLKQSGAWVNILKQKGFEDISNHFIDC
                     IENNIKPAINGEECIKAQRLLEKIINSVK"
     gene            5852..7021
                     /locus_tag="PC414_001656"
     CDS             5852..7021
                     /locus_tag="PC414_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDF2464053.1"
                     /translation="MKMFKLEEQKLKDLGAIITTNEIKQQPELWLETYEIYKSNKEKL
                     SRFIDTISNNHGQFRVIFTGAGTSAYIGNSILPYLKNKNDIRKYIFEAIPTTDIVSNP
                     YDYLKKDIPTLLISFARSGNSPESLAALNLGNKIVDNFYHLAITCNPEGELAKMTKND
                     ENNYLLLMPSKSNDEGFAMTGSFSCMMLSAMLIFDSLEDDVEKSYINAIIEMGRNVID
                     RKDEIHELINKDFDRVVYLGSGGLGGLTQEAQLKLLELTAGKISTVYDSPMGFRHGPK
                     SFIDENTLVFEFVSNCLYTRKYDLDVLEEIKRDKIAKFTCAVSVENENNFSGTKFEFK
                     EKYNKLPDVYLAMPYILFAQTIALFVSVKVGNKPDTPSATGTVNRVVKGVTIYEY"
     gene            7070..7921
                     /locus_tag="PC414_001657"
     CDS             7070..7921
                     /locus_tag="PC414_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HDF2464054.1"
                     /translation="MALVTTTSMLKKAQDEGYAVGAFNVENMEMVIAVIEAAEELNSP
                     VILQTTPSTVKYAGLDYYLANAKVAAERVNIPVAMHLDHGSSFDLAMKALRSGYTSIM
                     IDGSHESFENNISISKSVVKACSPSLIPVEAELGKVGGKEDDLDGGNSNDYTNPKEAK
                     EFVDRTGISSLAIAIGTAHGLYKGEPKIDLDRLSEIKNVVSIPLVLHGGSGIHDDIIK
                     EAIKRGIAKVNYATELRIAYSKGVKKVLDEDSEVIDPKKYGLAGLQSVKDFVKEKIKV
                     CGSVNRV"
     gene            8247..10148
                     /locus_tag="PC414_001658"
     CDS             8247..10148
                     /locus_tag="PC414_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2464055.1"
                     /translation="MRERIILETLKNSSGYLNIDYFADKLGVSTRTIRNEIKKIETIK
                     ECNGFKLEYKSKLGYILNIKDQDKFEHYLRNLPSDLVENPEQRLESIIVELLVNEGYK
                     TIEQLSKKFLVSSSQIKNDLKKIDEKIKDTELKLERKAHYGIKIEGSIKEIQSILVDS
                     YFRGNRNITEYRNKFIDNLKLANIRSTIKNVLNEHDLEANLTELEEILAQIIILYIRV
                     SMRVVGNASDLKLYTEDLIIDKLLDKIFRDKKYNLNYDEKYYLKELIKLKTKDKKATI
                     KNIDKNKLQDIMFEFFREIDKKYNSNFLEDKEFFNLFYLHIACLIERIKKNHKIINPF
                     SVKISQQYPTVFNLSIQFSKIIENEYHIKISQDEIGFIATHIAVPFEKREEANFNKKY
                     KIAIICSSGGGSAFLINLRLKEIFPNAEIRNFSLLEEKAVIEFAPDLIFSITNLLFEI
                     NAPVILINEILDELDYLDIKESVRFADHIGNISSPKQYILGLFDKNHFRCIKDKAKYK
                     DILDSMSKCIVDEGACSPTYPKDVWERESYLSTIYTNGVAIPHPIEMTGNKNIISVAL
                     IQTDIIYENRVPKIIFMISLIKGNLELHKQISKYLSKIMANKDMVDMLNKSQSYEEFM
                     YKLKIYLGG"
     gene            10151..11194
                     /gene="ypdE"
                     /locus_tag="PC414_001659"
     CDS             10151..11194
                     /gene="ypdE"
                     /locus_tag="PC414_001659"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDF2464056.1"
                     /translation="MNIEFFKRLSEADGIASNEEEVRHVLLEELKAYSDKIICDGLGS
                     IIFSKIKDESAPNVMICAHMDEVGFMVRSIDKLGMIHLITIGGVKPLAQFVQKVRITT
                     KEGKKIPAVINATYNNGKAENIYADIGAYTEEDVYNLGINVGDMVTYTTSFEEFTLPD
                     RLVGKAFDDRIGCFVMGEVLKELRKENLNCNIHFAATSSEEVGIRGAKTSTQLINPDI
                     VFVIDVACAKNEFVRDYTNKKQIDKGVMLMHRDRTLVPSRKMIDYVMEIADKNNIPLQ
                     HDMFESGGTDGGEAHLVNEGKPCVVTCVPVRYGHCAFSIVSNKDLENIIRLYTQLILN
                     FDEEQYKHIRNFI"
     gene            11209..11514
                     /locus_tag="PC414_001660"
     CDS             11209..11514
                     /locus_tag="PC414_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF2464057.1"
                     /translation="MSTEVQIMELISTAGESKSKAFEALKKVKSKDFKGAKLLIEEAR
                     KIDIEAHRIQTELITQDLNEDVESPAMSLLMVHAQDHYMTSQLAKDLIEALIYCLDK"
     gene            11611..11946
                     /locus_tag="PC414_001661"
     CDS             11611..11946
                     /locus_tag="PC414_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2464058.1"
                     /translation="MKILLCCAGGFSTNMLMQNMKKVVKESAKLNEKDFEFTAIPADS
                     IESEVDDWDIVLIGPQIAHKIDFLKGILEPKNKPFAVIDKDVYGKMDGATVMKLALIT
                     YKKHQLAQG"
     gene            11978..13300
                     /locus_tag="PC414_001662"
     CDS             11978..13300
                     /locus_tag="PC414_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2464059.1"
                     /translation="MFSKFEAFMNRVFMPLAHKVDNQRHLGSIKAGMVAMTPFTILGS
                     IFAILPALPNMLGENNPISKFILSNSAILDLPVSLSIGLIGLYACMCIAFNLGNYYKL
                     YIPGCVALSTFSFLFLVASFTPDGALEIGNLGAKGLFTGMIAGLGSVELYNFCNKKNI
                     TIKMPENVPDFVSRSFELIPTTIIVCGTFIVARFISLKVFGELPPQILTQFLSPLVGS
                     MDNPWSVLALNFAICTIFFFGIHSSVFSPITRPIMITFIAENIAAMQAGETIPHFFTA
                     GASSAFFGFTGCGISIGCVIACMLSKNASYKKIGRVSLFPALFGINEPILFGAPIILN
                     PIMFIPHVFGGAIIGTLPMFFMHWGWLAKPVFDPPYVGVFLEGFLTNGDWHSILANAL
                     QLVLAVLLYWPFFKIMERQQNNKKQKEETKNIFSKEEEGLLDDLGLDF"
     gene            13310..14380
                     /locus_tag="PC414_001663"
     CDS             13310..14380
                     /locus_tag="PC414_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF2464060.1"
                     /translation="MNNTMERLVSLIYKRGVDALLIKAKNTKRYIGALSGSGVYVLVT
                     KDKNYQILDGRYIDEADKKTTGFIKRIVSQGSYIPTIIELLKELNIKKLAIENQAMSI
                     QEYLSLKNEGFEINLITNELWKLRAIKSKEEIKLIKKACEITDEVFDEVISEIKKDMT
                     ELEVSALIQYHALKKGASGMSFETIVVSGERGAMPHGRPTNKKLKVNEAITIDFGVVY
                     QGYQSDMTRTISIGKPPKIIKEIYDVVLEAQLSAIESIKEGTRASDVDKVARKIIDKH
                     GFGEYFNHGLGHGIGLGDGEVPTLNPNSEDILVEGMVMSCEPGIYIPNVGGVRIEDDI
                     VIIDGKGVPLNKTSKEFIILGE"
     gene            14385..15572
                     /locus_tag="PC414_001664"
     CDS             14385..15572
                     /locus_tag="PC414_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2464061.1"
                     /translation="MKYNFNKVLNRKEGNCRKWSNHVIKEKFGLNEDAIPMDLADIDF
                     ECAPAIKESILKRASVGDYSYTFIGDDFYDAIINWNKRRFDVYIEKDWIKLTFGTVST
                     LHYIVQAYTKEGEGVLINTPAYDPFAEAVVNNNRKLCCSSLKLDNNRYYLDLEDMENQ
                     MKYENIRLLIFCSPQNPSGRIWTKEELYQVSELCLKYNVILVSDEIHRDIIFKDYRFV
                     SLWNAHPEIYKNSIICVSPNKGFNLGGLKTSYVLIRDVKIRETLLERLKSNSITSPNV
                     FAIPAIVAAYNESEEWLDAMTSYVEENFEIVYDFFETNIPKAKVMKSDSSFLAWIDVR
                     EVFRNEEESKEFFRHANLTMVVGSYFVKDGDGFIRINVGCPRETLNEALNRIKKTYIS
                     MYC"
     gene            complement(15769..16641)
                     /locus_tag="PC414_001665"
     CDS             complement(15769..16641)
                     /locus_tag="PC414_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HDF2464062.1"
                     /translation="MNILQNENLIIESSSFGAELTKIFSKKYDKNILWDGNKKYWGRQ
                     SPILFPIVGKLFDNETIIEENLYSMTQHGFARDMDFEIVDKGDTFVTYKLTDNESTLK
                     KYPYSFELIINYTLNDNSIEVEWIVKNTDSKDIFFSIGGHPAFNLPFYEQNDFSKHYL
                     EFKSKNDVEKINLNGSFTDDIIPIGKLKNLQLNPEVFKNDALIYTNIDEVSICNNDGS
                     KYVTISMEDFPLVGIWTPYYSETNSTAPFLCIEPWYGLADSINSNKIYKDKKFINKLS
                     KGKVFTASYNINIH"
     gene            complement(17035..17226)
                     /locus_tag="PC414_001666"
     CDS             complement(17035..17226)
                     /locus_tag="PC414_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464063.1"
                     /translation="MDDFSKLKSLYEDGYRCIYHDCVDNNYTIYLKNFYTEGSETIEL
                     SSESDFSQFKDYIDGLRMS"
     gene            17446..18492
                     /gene="cobT"
                     /locus_tag="PC414_001667"
     CDS             17446..18492
                     /gene="cobT"
                     /locus_tag="PC414_001667"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HDF2464064.1"
                     /translation="MSLLESISKNIYSLDNSSIEKTKQRLDRLIHPTGSLGKIEDICM
                     QLAGIFGNENFDTSKKVIIAFAGDHGVYEEGVAPDPQNITKLQFPNFSKGLCGVGVIS
                     KFVGADVVAVDVGINCDEKLDGVLDYKIRKGTSNMAKGPAMSKQEAIRCLEIGIEIAE
                     QCIEKDYKVIGIGEMGIANTTPSTAIISVISGCDPLEVTGIGAGLKKERLKHKAEVIR
                     KAIEINNPNPTDGVDILSKVGGFEIGSMAGVILGCSANRIPVVIDGFISYAAALIAYK
                     INPKTREYMIASHLSAESGTKRALDILKLDPLLNMDMRLGEGSGAALAFDIIEASNYT
                     YKNMATFDEIDMGR"
     gene            18524..19084
                     /gene="cobU"
                     /locus_tag="PC414_001668"
     CDS             18524..19084
                     /gene="cobU"
                     /locus_tag="PC414_001668"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HDF2464065.1"
                     /translation="MSKIILVTGGARSGKSNFAEKLCFDRNNNTAYIATSIPFDDEMK
                     DRVKKHQENRPRNWNTYEIYEDIYSIIKEIYKEHETVILDCVTLLVNNLMFEHNLNIE
                     ESSQEEINKLEQYIKEQVVKLIEEIEKTNLYFVFVTNELGMGIVPGNKLSRIYADIVG
                     RINQYIASKSNEVYFVVSGIAMKIKE"
     gene            19094..19867
                     /gene="cobS"
                     /locus_tag="PC414_001669"
     CDS             19094..19867
                     /gene="cobS"
                     /locus_tag="PC414_001669"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HDF2464066.1"
                     /translation="MKRFILILQFLTRIPIKLNVGFDDEFYKSIVYFPLVGFVIGILS
                     YLIGWISMLLFEPFIASIIITLAGVLITGGLHIDGLGDTFDAIYSYRDKEKMLEIMKD
                     SRLGTNSLLAIMFVLLLKVGFVYDIISNNSLWVIIFMPMIARLGVMLLTYKTVTPREK
                     GMGNLFIGKLTTSMLITAIIYTLLIVALITKFIFLLPNIVLIKVLGSIIVVFVFIILF
                     KKHIYKKIDGVTGDILGCGIELSELVYLIYIYLLIFMFF"
     gene            19887..20501
                     /locus_tag="PC414_001670"
     CDS             19887..20501
                     /locus_tag="PC414_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDF2464067.1"
                     /translation="MIRLILIRHALTNDNKKGRLSGHINSCISEEGKLQINKITRYLS
                     NENIDKVYTTPSTRTKDTVEKISKLKLLEIEEKEALREISFGDFEGRTFEEIKIKYPN
                     EFEKMIKEGNNYRYPNGESLIDSYKRVAKEIDNIILENNSNLDTKTILICSHAGTIRN
                     IITHLISGSYKYHWNFKIDNASITVLEIDGGFAVIDKMNFTDFI"
     regulatory      20568..20747
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            20877..22376
                     /locus_tag="PC414_001671"
     CDS             20877..22376
                     /locus_tag="PC414_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HDF2464068.1"
                     /translation="MLQGTASNVGKSVLVAGLCRIFKQDGYTVAPFKSQNMALNSFIT
                     KEGLEMGRAQVFQAEAAKIEPIADMNPVLLKPSGNNKSQVIVRGKVVGEMPSSKYHDY
                     KLELVDVLKETFNSLNSRYDIVVMEGAGSNAEINLKDRDISNMGMAEIADAPVIIVGD
                     IDRGGVFASLAGTMLLLNEDEKRRVKGVIINKFRGKKELLQDGVKMLEDIIKVPVIGV
                     VPYTDIKIEDEDSVTTRFKKKMNKSDIHIEIIRTPHMSNFTDFNIFETQEDVSIRYVD
                     YGESLGNPDILIIPGTKSTIDDLTYIRKSGLESQIKNLHSQGKLIFGICGGYQMLGKK
                     LKDPYHVESEIEEVDGIGLLDTETTFEKEKTTTQVEATIVECISEYMNNLKGKKVKGY
                     EIHMGITKRGNDVRSLDLITKKLDKEVNYTEGSVNKEGNVIGTYLHGIFDEISFTRAI
                     LNNIRKKKGLGELESKVNSFNEFKDKEYDKLADFLREHLDIEKIYEIMK"
     gene            22415..23791
                     /locus_tag="PC414_001672"
     CDS             22415..23791
                     /locus_tag="PC414_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HDF2464069.1"
                     /translation="MKKILIAGTNSGVGKTTISLGIMQALVKRNMKVQPYKVGPDYID
                     PSYHTFITGRHSRNLDSYMLDDEKIKYIVNKSSKDADISVIEGVMGLYDGFGIDLDNC
                     TSSHTSKVLKSPVILVINGKSMAASSAAMVLGYKELDKDVNIKGVIVNNVKTNSHYQI
                     IKSSIEKYCNVEVLGYFPPNNKFSLESRHLGLVPSVEMKSLREKFYTLADEIEHYIDI
                     DRIIEISESDEFESDFDFQTLIENNKIKEHVNNKSIAIAYDKAFNFYYRENIELLEEL
                     GLEINYFSPLEDVSVPNADYIYIGGGFPEIFGKELEANESMRTSILEAHNNNIPIYAE
                     CGGLMYLGEKLLNQEEQEFNMVGIFSGISKMTSSLKRFGYCIGVAKENTLLSKKGESI
                     KGHEFHHSIFESDEKCVYSMRKERDGNIVEEWDGGYSKGNTLATYLHTHFYNNLNCIE
                     NFLKKGNE"
     gene            23792..24763
                     /locus_tag="PC414_001673"
     CDS             23792..24763
                     /locus_tag="PC414_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HDF2464070.1"
                     /translation="MSILSIYIGYVTDLIVGDPYSFPHPVRFIGKLINFTQGIIRKVS
                     KNDRQLKFGGFILWFITVGITYLTTYAIVKLFSFNILLSIVVNSFIIYTTLATKCLKD
                     EALKIYNVLKTEDIEKSRIQLSYIVGRDTTNLSEPEIIRATVETVAENTVDGIIAPMF
                     YAFIGGAPLAMAYKAINTLDSTVGYKNKKYKDIGFASAKIDDIANYIPARISVILMTI
                     GSFFLNYNYRNCFKISIRDRKNHKSPNCAFSEGAVSGALGIQLGGTNVYFGEKVYKPT
                     IGDKLREIDKEDIIKTNKIMYASSFVSMLVFTLIYVLCRMILINLII"
     gene            24795..25865
                     /locus_tag="PC414_001674"
     CDS             24795..25865
                     /locus_tag="PC414_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF2464071.1"
                     /translation="MKDLGHGANVDEMARLYGKNPKEIIDFSSNINPNVLPNLERYIL
                     KGLEECRNYPDINYTNLRENISKYIDINPDFIIPGNGATEVIYLLMKSIKKKLAIINP
                     TFSEYRRSAELNNLDIIDLELDLENNFKLNIDIIKENIKRFDSLFICNPNNPSGNVQD
                     LKELVHLLDKHNKVLIIDETFMEFVEDESKYSLVKYIESNKNIFIIKAVTKFFGMPGL
                     RLGYGLTSNTEIMNKIYEHKEPWTINSFADILSNFIFEDKEYIKNSKEYYIEERKYML
                     QELRNIRNIKVYDTDANFILIRIYKKTTKELKKDLFKQGNILVRDASNFIGLDDSFIR
                     VAIKSHEDNKILIENMKNLLGD"
     gene            25867..26754
                     /locus_tag="PC414_001675"
     CDS             25867..26754
                     /locus_tag="PC414_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HDF2464072.1"
                     /translation="MKSYGICPASCGEFVQGIIDDEEYLCSYAIDMYSKVYIEEKLVD
                     INLGRYKSRLAIEKVFEKFNLPKKYTKNISLNINSKIPVGKGMASSTADIGATIKATL
                     SLIDKDLSSEEISKLAAEIEPTDSIFIDKNSIFNPLNGTVIKYLGNLTNAKVVILEPN
                     KVLDTMKIRLRQDYNKLKVENKEVIKKSFALLEEGLKKNNLSLVGEACTLSSLANENI
                     EKKEYLNEIIKISKKYGAYGVNIAHSGTVVGILIDKFMNDKKMIDALCESNINSVYNK
                     IYTQNIINGGIKGEIEWNT"
     gene            26742..27374
                     /locus_tag="PC414_001676"
     CDS             26742..27374
                     /locus_tag="PC414_001676"
                     /EC_number="5.4.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003724727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-8 methylmutase"
                     /protein_id="HDF2464073.1"
                     /translation="MEYIKNPMKIEEKSFELIQEIIDEIRPDYKFKNNIEEKIIKRAI
                     HTTADFDYLDILKISEEAVDSIIDALKNNASIYTDTNMALSGINKRKLESLGCKYKCL
                     VADDETVKLAKEKGITRSMAAVEIAAKEEGRKIFVLGNAPTALYKVMEMKSEGRLDLD
                     AVIGVPVGFVGAQESKDEVEKTDIPYIISKGRKGGSNLAAAIVNAILYTM"
     gene            27383..28609
                     /locus_tag="PC414_001677"
     CDS             27383..28609
                     /locus_tag="PC414_001677"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HDF2464074.1"
                     /translation="MEEYVYIDGKKYRRGYTTGSCATGASKAAVYMLITKNRINTINI
                     DTPKGIPLLLKVDNINISDTFVECSIKKDGGDDIDATHTMDIYARAEIVAKNDKNKGY
                     LTLKDIDNLSTNSECKSELYKFIRVYGGTGIGVVTKKGLSVDVGKPAINPTPLKMINH
                     EIRKLIGDNFESILGNDKVLKITIFAPQGETVAKKTFNPRLGIVGGISIIGTTGIVEP
                     MSDEGWKKSLSIELQMKKEQGLDKIILVPGNHGEQFIREKLNLDIKYVVRVSNFIGYM
                     IKEAQRIGYKKILMAGHIGKFIKVSAGIFNTHSKVADARSEILVANLALMGARYEFLN
                     KINQCVTTEEAVELINNSEYREVYNILSNKCRERVKQYLNEDSDDIDVEVIIFSMDKS
                     LLGKSDNTDDLVEVFI"
     gene            28606..29214
                     /locus_tag="PC414_001678"
     CDS             28606..29214
                     /locus_tag="PC414_001678"
                     /EC_number="2.1.1.289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-7 (C(5))-methyltransferase"
                     /protein_id="HDF2464075.1"
                     /translation="MINIIGLGPGNLDYITKKGENLISTSDVLIGGKRNLESIKNFEG
                     EKIVLDSNLREIIEYINNNKEKQISIIASGDPLIYGIGRYLSKNIDNKMLNMVSGISS
                     LQYIFSKIYVDMNDVYITSSHGKVPDFDYILSHKKVCMVTDSKIGPKQISREIIDRNL
                     NKIIVVGENLSYDNEKITIAKPEEIIRMDNFDMNVVVILDEE"
     gene            29204..29779
                     /locus_tag="PC414_001679"
     CDS             29204..29779
                     /locus_tag="PC414_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating cobalt-precorrin-6B
                     (C(15))-methyltransferase"
                     /protein_id="HDF2464076.1"
                     /translation="MRNSEFITGKVPITKEEVRAISISKLNLVNAKNLIDIGAGTGSV
                     SVEAAYNYPNLKVISIEKNDDAIELIEKNKKKFNLKNIEVIKGYAPINLGLNVKVESI
                     FLGGTGNNLEEIIEWSKKNLVTGGRLVANFIIIDTFNQTLKLLKKHGFKEIDVCVLNV
                     SKLEKLGKGEYFKPLNPIYIISCEKGENDDE"
     gene            29772..30524
                     /locus_tag="PC414_001680"
     CDS             29772..30524
                     /locus_tag="PC414_001680"
                     /EC_number="2.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-4 methyltransferase"
                     /protein_id="HDF2464077.1"
                     /translation="MNKVHFVGAGPGDKELITLKGYKLLSNADVVIYAGSLVNPELLE
                     YCKEDCQIHNSAHMDLQEIIDVMREGIENNKSVVRLQTGDFSIYGSIREQVEDLNKLN
                     IDYDCTPGVSSFLGAASSLGVEYTVPEISQSVIITRMEGRTPVPEKESIQSYAKHQTS
                     MVIFLSVQEIEKVVSKLLEGGYPKDTPIAVIYKATWADEKIVKGTLSDIAVKVKENNI
                     NKTALIMVGRFLGEEYNNSKLYDKDFKHEYRG"
     gene            30508..31629
                     /gene="cbiG"
                     /locus_tag="PC414_001681"
     CDS             30508..31629
                     /gene="cbiG"
                     /locus_tag="PC414_001681"
                     /EC_number="3.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin 5A hydrolase"
                     /protein_id="HDF2464078.1"
                     /translation="MNIEGNIAIICITENGKKLALKINSLVNNCVIYQVKNKKSNLVV
                     EETCVHTVQKKLSDFVGEIFSKFDYIVFIMATGIVVRSIAPYIISKFSDPAILVMDEK
                     GKNVISLLSGHIGGANSLTQYISSLIDANPVITTATDINQKASLDMIAKKLNAHIDNF
                     RESVKEVNSMLVNNKCVGLYVDEGYQVDTRGFKLIESKDELRDSFSNTYIEKLVVVSN
                     KINCIEDIENLNLDNKFEHCYSTQKIIKVIPRDIVIGIGCRRNTDSELMYNSLEEFLN
                     KQNIDIRAINTVGSIELKKDEKAIIELSKILKVPFKTFSVDEISKVDNLFDKSDFVKK
                     NVGVYSVAEPVAYLLSKGNLLVEKHKYKGITFSVGRIKI"
     gene            31626..32351
                     /gene="cobJ"
                     /locus_tag="PC414_001682"
     CDS             31626..32351
                     /gene="cobJ"
                     /locus_tag="PC414_001682"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011701973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HDF2464079.1"
                     /translation="MIYVVGIGPGSKDTMTLEAIKAIEDSEAIVGYKTYIKLIEEFIQ
                     GKEIIQNGMRQEVDRCKQAVEEAKKGKKVAVVSSGDAGIYGMAGLILELISKEDEDIK
                     VKVIPGVTASIGAAAILGAPIMHDFCHISLSDLMTPWEVIEKRLRLAAEADFVICLYN
                     PRSKGRSEHLSKAFKIMGEFKGGDTPVGVVKDVGREKEEKFVCTFENMDFEKVDMTTM
                     VIIGNKSTYINGELMITPRGYTV"
     gene            32348..33100
                     /gene="cobK"
                     /locus_tag="PC414_001683"
     CDS             32348..33100
                     /gene="cobK"
                     /locus_tag="PC414_001683"
                     /EC_number="1.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A reductase"
                     /protein_id="HDF2464080.1"
                     /translation="MILILGGTSDSLSICDKINELKNQPYILSVTTDYGRELAQQHAE
                     NVILGKLGKEDMLKFIKDNNIVKIIDATHPYAVEVSKTAITCAKLLNIDYIRFERKSL
                     IEEIDYDNKFIVDTIEEACEIANKIGTNIFIGTGSKNLNRFIEEISDKNLVVRVLPTS
                     EVILSCEKLGLSADNIVAMKGPFSQYINEETYKHYNIDLVITKESGIAGGFLEKVNAC
                     KTFNIPVIIIKRESINYENVFNDINKIKDILV"
     gene            33119..34597
                     /locus_tag="PC414_001684"
     CDS             33119..34597
                     /locus_tag="PC414_001684"
                     /EC_number="2.1.1.151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-factor II C(20)-methyltransferase"
                     /protein_id="HDF2464081.1"
                     /translation="MKKALLVVSFGTSYHETREKTIDICEDKIKNSLKSHDFFRAYTS
                     NMIINKIKKRDGIEIDNPIQALDKIYEQGYDEVIIQTLHIICGEEFNKLKEQVDGYSS
                     KFKKIVLGRPLLTHIEDYQEAVEAIKHQIPHMESNEAVVFMGHGTLHESHSAYPALEY
                     MLRDNGINAYVGTVEGYPEIEHVIRRLKEGNIKTVNLMPFMLVAGDHAINDMAGDEED
                     SWKTILEENGFNVRVHLKGLGENSYIQDKFVRHAVKCAENAKFYGIGAGPGDGSLLTI
                     KAVNTLKKLDILYTPESKKGGDSLALSIVSEYLPESVEIKSRHFPMSFDGNEKSLAWD
                     NVAQEIISDVNDGKNVGFVTLGDPMIYSTYVYIMKRILGEVEVETIPGISSFSNIASN
                     QNFPLVMDKEALVVIPCTMEDDKIEYALQNYNSIVLMKVYKNFKEIIKKLEKYDLIEH
                     AILVSNSSQESENVFTDLKEAHLEDKISYFSTILVNKDNKIK"
     gene            34627..35532
                     /gene="hemC"
                     /locus_tag="PC414_001685"
     CDS             34627..35532
                     /gene="hemC"
                     /locus_tag="PC414_001685"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HDF2464082.1"
                     /translation="MNIVVGTRGSNLALIQTEWVINELKKKYPEISFEIKIIKTKGDL
                     IQNVSLDKIGDKGLFVKEIEQQLLDGKIDIAVHSMKDMPSYLADGLKFAHTPKREDPR
                     DVLILREGYKNLDDLPHGAVIGTGSKRRKFQLLKQRPDLNIVQVRGNVETRIRKIKDE
                     NMHGIVLAASGIIRANLQDKISSYLPVDVVIPAPAQGALAIEIRSNDSTIEGIVNSLK
                     DENTEIQILAERGFLDGVNGSCHIPMAAYCEIIQDKIHLTGLYGDSEGKKVVIKSIDG
                     DISSPRELGLKLAKLVLKEYENYEG"
     gene            35522..37021
                     /gene="cobA"
                     /locus_tag="PC414_001686"
     CDS             35522..37021
                     /gene="cobA"
                     /locus_tag="PC414_001686"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HDF2464083.1"
                     /translation="MKGKVYLVGAGPGDYKLMTLKGLECIRKSDVIVYDRLANSSYLR
                     EAKPDCELIYVGKASSNHILPQEDINRIIAEKAKEGKIVTRLKGGDPYVFGRGGEEAE
                     TLVDEGIEFEVVPGITSAIGGLCYAGIPITHRDHASSFHVITGHLKGDDSGELNWNAL
                     ANNKGTLVFLMGISNLEKISENLMKEGKDKDTPVALISWATRYNQRVVTSTLENVYET
                     AIKEEVKPPTLIVVGSVVGLREKLNFFESKPLFGKSIAVTRSRNQNSVLVEKIMDLGG
                     NPIEIPTIKVEKIQNNINLENEIKNINKYNYLILTSKNAVEIFFEKIYEMNFDLRILS
                     NLKVCAIGSATSNELKKRGIIADIVPKKFVAESLYEELAPILNDDDNILIPRAENARD
                     YLVDKLKAICEVTEVHIYRTVIDEEKKGEILDILNSNDIDYITFTSSSTVNNLVEIIG
                     KENIKILEKIKTLSIGPITTETINSLGIKLYKESATSTIDSLVDLIIKN"
     gene            37038..38003
                     /gene="hemB"
                     /locus_tag="PC414_001687"
     CDS             37038..38003
                     /gene="hemB"
                     /locus_tag="PC414_001687"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HDF2464084.1"
                     /translation="MLRRPRRLRASKAIRNLVRETKVNVEDLIYPLFVVEGENIKEEI
                     KSLPDVYHLSIDMLEDEIKEISELGIEHIMLFGVPDEKDECASQAFNDNGIVQKAIRK
                     VKEINSDINVITDVCMCQYTSHGHCGILTDTGYVDNDKSLEYLAKISVSHAKAGADMV
                     APSDMMDGRIQAIREALDENGLENVGIMAYSVKYASNYYGPFREAAHSAPSFGDRKTY
                     QMDPANSNEAIIETELDILEGADILMVKPALSYLDIIRRVKDNFDLPLAAYNVSGEYS
                     MLKFAVKEGLLNEDVILESITSIKRAGADIIITYFAKDIAKLLKK"
     gene            38156..38734
                     /locus_tag="PC414_001688"
     CDS             38156..38734
                     /locus_tag="PC414_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HDF2464085.1"
                     /translation="MLYPINLKLDELDVVIIGGGEVAYRKCKNFLEFNKNVTIVSKQI
                     LNKFYDLKGNIKIIKDDYKEIYIKDASVIIAATNNRELNMEIGLYCKAKNKLVNVVDN
                     IEISNFTVPSYIKRGDLLISVSTGGKSPSLSSKIKKEIEERYTEDYEEYLNVLGDIRK
                     KVIKKYEDKSKRKDVLNMLVTLDLEELKKFDI"
BASE COUNT        15481 a         4090 c         6836 g        12365 t
ORIGIN      
        1 caacaatatc aaaaatattt taaaagaaaa tcatataacg tttgaagaat atgaccatga
       61 acctatttta tcatttgaaa aagctaaaga aataagagaa aaattacaat atactggagt
      121 agaaagtaaa agtttattta taaaagatag atctggaaat tactatatat ttgtaaccac
      181 agaaggaaaa aaattagatc caaaaactat aaaaaatgtt ttaggtggaa aagtatctat
      241 atgttcaaaa gaagaattaa cacaaaaaac acaatgtttg cctggatgtg tatctccttt
      301 tggatatgag aaaaatatct ctcttgtaat agatcaagat atatttaacc atgataaaat
      361 aatcttttca ccagggatac cagaaaagac atttattatt acagctagtg gatttaaaaa
      421 agtaataaac aatataaaca atacaactta taaatatata tattaatgaa ttttaatcat
      481 aattgtaaat tagagaaaat aacattagaa atcatgctta actatagtgt aaataaatta
      541 tattcaacac aaaagattga tactattatt acaatagtta ctgttaagct caaattcaat
      601 gtattatgag ttattatttt taaattttaa ttacaaaagc agggtagttt taactactct
      661 gctttatatt taacataaaa tatataaaat caacaaaatg attttagtct atctattaga
      721 tattcttcgc tctcaatgct taaaactttt agatatatac aataagtgct ttcatttgta
      781 aaaatttcta tagtattatc ttgtttattt acacctacaa tatctttaaa attgatatga
      841 tttttagtat ttttacattt gaaaatatat ctgttatcaa tcaaaatctc atcattagta
      901 attgaaatat agggtttatc tcatgcttta ttaaaaattt atatgtttgc tcaagataaa
      961 aacttacaaa taataataga aaagttactt taaatgtaca tattatcgaa aatattatca
     1021 caaaaatact tataatatat atttgtttaa aataacctct atcgcaatag tttattaacg
     1081 atatggaaat aaaagtgtta aatcagattt tggagaaata actttaaata tttccagact
     1141 ctttttagac agcctccata accttatatt gaacctattt attaactttg tacttacaga
     1201 taccatttat ataagtttcg tgtatattaa agtcattatc tgttactata aagtctgcat
     1261 ccataccttg atatatagag ccacagacat tatcaatatt acaacttaaa gcagggacat
     1321 atgtagccat ttttattgca tcttctaaag ttgatatgtt ccaatcaact atatttctca
     1381 cagccttgtc catagttaat acacttccag ctaaactccc actatttaat cttgcaacac
     1441 tattttttac atttacatta aacttaccaa gagtatattt tccatcctcc atagcaccag
     1501 ctctcataca atctgtaatt aaagctatgt tattactaga cttcgcatcc atcattattt
     1561 taacagctat tgggcttaca tgatgaccat cacaaattaa ttcagcataa gtatctttta
     1621 gtaccatagc agccccaacc attccaggtt ctctatgatt taaaggactc atcccattat
     1681 atgtatgtac aaacacttta gcacctgaat taacgacttg tttaacttgc tcaaatgtag
     1741 ctgaactatg accaagagaa actgatatat tatttttagt tgcatatctt gttaattcta
     1801 ttgaaccttt acgttctgga gcaattgcta tttttcttat taaatttttc gatttttttt
     1861 gccataattt taatatattg atgtcagggt ctttaaaata tttttcattt tgagcgccct
     1921 tatatttttc tgtaaaaaat ggaccttcta aataaatacc ttgtattttt gcaccctcaa
     1981 ctcgattgaa gctattgctt atattttcta cagaatcact tagtgtatct acagaatcag
     2041 ttaaagttgt aggtaggaaa gatgttactc catattttaa taagcctttt gaaattgtgt
     2101 ttattgcttc aaatgtattg tccatgacat ctgcacctaa aagaccgtga atgtgagtat
     2161 caactaagcc aggagcaata tatttacctg aaaaatctat aacattatca gtaacattag
     2221 gtctttcttg ttgaaattca ccgaatttgc cattaattat actaaggtaa cctttttctt
     2281 tgattccatg tttcataaaa aatttatctg cataaataaa acttttcaaa ataacctcct
     2341 tataattata ctatcattgt ggcattgacg tttataatat cattctattt tcttgaaagt
     2401 tatttaactt actatcttac atgtcttact ctatatacaa atttatcgct tctagcaaca
     2461 ctcaaagtaa attcaataac tatattgtca tcattatatg tggtacgaag taatttcaga
     2521 caaggtgaat ctcctggtac ttccaataac ttagcttcct tatcagagat tattccagct
     2581 gaaaattctt catcagcaac tttaataatc tcgccataat cttctctaaa tatttcatat
     2641 aaaggttttt gttcgatttt ttcttttgtc aatcctataa attctttcat aggaagatat
     2701 gttctttcaa gcatcatagg aaggtcatca gcaagtctta aacgcattat ttttatcatt
     2761 ttttcaccag gagttatacc taatttttca gagaaatact ttgtactttc tactacctca
     2821 aatgataata cctttgtacc tggctttttt ccttgtgttc tcatataatc tgtaaaacta
     2881 taactttcca ttaagttttg agaattttga cttagtgcag caacaaaagt tccttttcca
     2941 tgtcttttgt atatataccc catatgttcc aactcagcca tagctagtct aactgtggtt
     3001 ctacttactc catattcatc acaaattttt ctttcggaaa gcattctatc gtttggttcc
     3061 atctcatttt caatcttagt tcttaatatc tcaacaagct ggtcatatat tggtctttta
     3121 tcatttcttt ttatcataaa aatatagtcc tttctaagtg gttattacca caatttgatt
     3181 atagaatata gaattattta tgtcaatgaa taaagtttaa tcaaaattca ataagtaagg
     3241 ttattaattt attataaaat tatggtacat tgacaaaaca agtaaaataa tttatattca
     3301 aattggtaat aaccactttg ggtatattat agatgaggta ttttttattt tacagattaa
     3361 tttattgttt agaaaagtaa aaattaataa tgaggagaat ctagtatgat tacagtaata
     3421 acattcaacc catctataga tagaatgtat agagtgaata atataaacat aggtgaggtg
     3481 caaagggtag taagtaagaa tgcaactgct ggaggtaaag gattaaacgt agctaaggta
     3541 tgtaaaattt taaaagaaaa tcctttagct atgggatttc taggcggatt taatggagaa
     3601 tttataaaaa atgaacttag aaaactagat atacaaaata aatttacaaa aatagagcag
     3661 gaaactagaa attgcttaaa cataattgag gacaataaag ttagtacaga atttttagaa
     3721 aaaggaccaa tagtagaaaa aagcgattta gagagatttg aaaatgatat agaggaagta
     3781 ctaaaaaata ctaaaatact ggtagcttct ggtagttatt gtgagaatat gccgatagac
     3841 tattatgaaa agataggaaa tatatgtaga gcaaataata taaagtttat attagataca
     3901 tcaggagaag cattaaaaat agctttaaaa tcaaaaccat atttaataaa accaaatata
     3961 gatgaaataa gacatttatt aggaatgaat atagaatcaa gagaggaaat tatcttgtca
     4021 ggaaaaaaac ttattgaaat gggtgcagag aatgtatgta tatctttagg aaaagatggt
     4081 atggtgtatt taaacgaaga taatgtatat gatgtaaaag taccagttgt aaagtgtata
     4141 aatactgtag gtagtggaga tagcacagtg gctggattta gtgtaggtat tttaaggggt
     4201 tataaaatag aaaatttact taaatttgca aatgcttgtg gtataagtaa tgctttaaat
     4261 atggaaactg gatttgtaga tttgggagaa attgataaat atcaagattt agttgaagta
     4321 aaaaagttaa gttaaataca tttaatggga atataataaa tttaatgttg ttgtaataga
     4381 ttttaatttt aatataaaag atatagtctt atataaaaat aggttagaaa aattcatttt
     4441 taacctattt ttataataaa tatctatttt actgaattta taattttctc tagtaatctt
     4501 tgagctttga tacactcttc tccattaata gctggtttta tattattttc tatacaatca
     4561 ataaaatgat ttgatatatc ctcaaaaccc ttttgtttta atatatttac ccatgcacca
     4621 ctttgcttta aagttaggtt tccaccttct tctatttcca gtacattaag attcttaact
     4681 ctttgaatct taccctttga aagaatttca acttgttcta actttgttcc agaatctcta
     4741 tgcatagagg tgtttataga gaaattttta cctttaagtt tatgacttac aaatataaga
     4801 ttcttattgt ctgttaaaaa taaatcctct ccactaattt caacatcttc atttgctaac
     4861 cataaagcag tgtcaataac atgtatatag tcatctataa gagtactgtc gaatctaaca
     4921 tttcttaagg aatttaatcc atgtttacat atattgatag atacaatttc tgtagcatta
     4981 tttttaattt ctttgtacat aggacagaat ctacggttga atccaaccat taaatttaag
     5041 ttttttttcg tacttaactc gataagttct tctccttgac ttactgttga agcaagaggt
     5101 ttgtctacat atacatgaac acctaaattt aataatattt ttataatttc gtagtgtgtt
     5161 tctgtagagc tatgtagaaa aatacaatca cattttttag ctagggattc aatagaatcg
     5221 aatggcatga ttctataatc actacagact ttttcccttt taactttatt tggagtaaaa
     5281 gctcctacaa actcaaatct ctcagactta gttaatatag gtaaatatgc cttttgagct
     5341 atggagccaa gacctatcat tcccattttt atatttttca taatactttc tcctaaggtt
     5401 tttatattta cagaattgat tataattcag acacataaat ttgtaaactt tgtatcaaaa
     5461 aataaattta aagcttatat aagtaaatta gcgaaataag aaatattgat aaagtaattt
     5521 tgtaggatat ccataaaatt gattgatata aatattttat gtattgacaa aaaattcaaa
     5581 ttaatttata tttggattag tggtaattac cactttggtc tgaacaagct tttatcgtaa
     5641 aataaaaata ctaaagtata tagagtatga ttttattaaa tttttaaaga taaactataa
     5701 aattaattaa aatctatagt gtatatttta tagtgagttt aagtagtact aaaaaatcta
     5761 agcaaaaaat tataaaagag tatatttttt tatagtttaa agtggttata acctcattac
     5821 caaatgtaaa cgttacaagt atgaaaggag aatgaaaatg tttaaattag aagaacaaaa
     5881 actaaaagat ttaggtgcaa taataacaac taatgaaata aaacaacagc ctgaattatg
     5941 gttagaaact tatgaaatat ataaatctaa caaagaaaaa ttaagtagat tcatagatac
     6001 aataagtaat aatcatgggc agtttagagt tatatttaca ggtgctggaa cttcagctta
     6061 cataggaaat tcaatacttc cttaccttaa aaataaaaat gacataagaa aatacatatt
     6121 tgaagcaata cctactacgg atatagtttc aaacccatat gattacttaa aaaaagatat
     6181 accaacatta ttaatttcat ttgcaagaag tggaaatagt cctgaaagtt tagcagcttt
     6241 aaatttaggg aataagattg tagataattt ttatcattta gctataactt gtaatccaga
     6301 aggagaactg gctaagatga ctaaaaacga cgaaaataat tacttgttat taatgccatc
     6361 aaaatcaaat gatgaaggct ttgccatgac aggaagtttt tcgtgtatga tgttatctgc
     6421 tatgttaata ttcgatagtt tagaagatga tgtagaaaag tcatatataa atgcaataat
     6481 tgaaatggga agaaatgtta tagatagaaa agatgaaata catgagttaa taaataaaga
     6541 ttttgataga gttgtatatt taggttcagg aggattaggt ggtttgacac aagaagcaca
     6601 actaaaactt ttagaactta cagcaggaaa aatatctaca gtgtatgatt ctcctatggg
     6661 gtttagacac ggaccaaaat catttattga tgaaaataca cttgtatttg aatttgtatc
     6721 taactgttta tatacaagaa aatatgattt agatgtttta gaggagataa aaagggataa
     6781 aattgctaaa ttcacttgtg cagtgtcagt agaaaatgaa aataacttct caggaacaaa
     6841 gtttgagttt aaagaaaagt ataataaatt accagatgtg tatcttgcaa tgccatacat
     6901 attgtttgca caaactatag cattgtttgt atcagtaaag gttggaaata agccagatac
     6961 tccatcagct acaggaacag taaatcgagt agtaaaaggt gtaacaatat atgaatatta
     7021 aaagataaga ttaaaatatt ataactaaat aatttgggag ggtatcaatg tggcattagt
     7081 aacaacaaca tcaatgctta aaaaggcaca agatgaaggg tatgcagtag gagcatttaa
     7141 tgtagaaaat atggaaatgg taattgctgt tatagaagct gctgaggagt taaattctcc
     7201 agtaatatta caaactactc catctactgt aaaatatgca ggtcttgatt attatctagc
     7261 aaatgcaaag gttgctgcag aaagagtaaa tatacctgta gcaatgcatc tggaccatgg
     7321 ttcaagtttt gaccttgcaa tgaaagcttt aagaagtgga tatacatcaa taatgataga
     7381 tggttctcat gaaagctttg aaaataatat atctatatct aaatcagtag taaaagcatg
     7441 tagtccttct cttataccag tggaagcaga acttgggaaa gttggtggaa aagaagatga
     7501 tttagatggc gggaatagta atgattatac aaatccaaaa gaggcaaaag agtttgtcga
     7561 tagaacagga atatcttcat tggctattgc tataggtact gcacatggat tgtataaggg
     7621 tgaacctaaa atagatttag ataggttatc agaaattaaa aatgtagttt caataccttt
     7681 agtattgcat ggaggttcag gaatacatga tgatattata aaagaagcta taaaaagagg
     7741 tattgcaaaa gttaactatg caactgaatt aagaattgca tatagtaaag gggttaaaaa
     7801 agttttagat gaagattctg aggttataga ccctaaaaag tatggattag cagggcttca
     7861 atcagtaaag gattttgtga aagaaaagat taaagtgtgt gggtctgtaa atagagtata
     7921 aaaataaaat atcctaaatt tattttagat aagtttagga tatttattat atgaaaaatt
     7981 tatattaatt atatgtaaat ggtgtatttt tttcatttaa ttgatgaaaa atatcagctt
     8041 ttattggtaa agtattacat atataattaa aatatactaa aaattgaact atatatttta
     8101 ttattataaa atatttatat taaattgtgg aattaaaata tactaggata taaaattctt
     8161 aaataaaaag aaaaggttgt ttttataaaa aatatttttt ataccacttt aaaaaacaca
     8221 ataataacac tgagggaggt ggggatatga gagaacgcat tattcttgaa acattaaaaa
     8281 atagtagtgg ttatctaaac atagattatt ttgcagataa gttgggggta tctactcgaa
     8341 ctatcagaaa tgaaataaaa aagatagaaa ctataaaaga atgtaatggt tttaaattag
     8401 aatataagtc taaattagga tatattctaa atattaaaga ccaagataaa ttcgagcatt
     8461 atctaagaaa cttaccctca gatttagttg aaaatccaga acaacggttg gagtctataa
     8521 tagtagagct tttagtaaat gaaggttata aaactataga acagttaagt aaaaaatttc
     8581 tagtaagttc atcccaaata aaaaatgatt taaaaaagat agatgaaaag ataaaggata
     8641 cagaattaaa actagagcga aaggctcatt atggaattaa aatagaaggt tctataaaag
     8701 aaattcaaag tatattggtg gatagttatt ttagagggaa tagaaatata acagagtaca
     8761 ggaataagtt tatagataat ttaaaattag ctaatattag aagtacaata aaaaatgtac
     8821 ttaatgaaca tgatttagaa gcaaatttaa cagaacttga ggaaatatta gcacaaatta
     8881 ttatattgta tataagagtt agtatgagag tagtgggaaa tgctagcgat ttaaaattat
     8941 atacagaaga tttaattata gataaattat tagataaaat ttttagagat aaaaaatata
     9001 accttaatta tgatgaaaag tattatttaa aggagcttat aaaactaaag actaaagata
     9061 agaaagccac tataaagaat attgataaga ataaacttca agatattatg ttcgaatttt
     9121 ttagagagat agataaaaaa tataactcta attttttaga agataaagag ttttttaact
     9181 tattttactt acatattgca tgtttaatag aacgtataaa gaagaaccac aagataataa
     9241 atcctttttc agttaaaata agtcaacagt atccaactgt ttttaattta tctattcaat
     9301 tttcaaaaat catagaaaat gagtatcata taaaaataag tcaagatgaa atagggttta
     9361 tagctactca cattgctgtt ccttttgaaa agagagaaga agctaatttt aataaaaaat
     9421 ataaaatagc aataatatgc tcttctggtg gaggtagtgc atttttgata aatttaagac
     9481 ttaaagaaat ttttccaaat gctgagataa gaaatttttc attactagaa gaaaaagctg
     9541 taatagaatt tgcaccagat ttgatttttt caataacaaa tcttttattt gaaataaatg
     9601 ctccagttat acttataaat gaaattttag acgaattgga ttatttagat attaaagaaa
     9661 gtgttagatt tgcagaccat ataggaaata tatcaagccc aaaacaatat atattaggtc
     9721 tatttgataa gaatcacttt aggtgtataa aagataaagc taagtataag gatatccttg
     9781 atagtatgtc aaaatgtata gttgacgagg gagcttgttc tccaacctat cccaaagatg
     9841 tatgggaaag agaaagttat ttgagtacta tttatacaaa tggggtggct atacctcatc
     9901 caatcgagat gacaggaaac aaaaatataa tatcagtagc tttgatacaa actgatatta
     9961 tttatgaaaa tagggtgcct aaaattattt tcatgatttc attaataaag ggaaatttag
    10021 aacttcataa gcaaatatct aaatatttaa gtaagattat ggcaaataag gatatggtag
    10081 acatgctaaa taagtcacaa tcttatgaag agtttatgta taagttaaaa atctatctgg
    10141 gaggttagac atgaatatag agttttttaa gaggttgtca gaagcagatg gtatagcatc
    10201 taatgaagaa gaggttagac atgttttatt ggaagaacta aaagcttata gtgataaaat
    10261 tatctgtgat gggctaggga gtatcatctt ttctaaaata aaagatgaga gtgcaccaaa
    10321 cgtaatgatt tgtgctcata tggatgaagt tggatttatg gtgagaagta tagacaaact
    10381 tggtatgatt cacttaataa caataggagg agtaaaacct cttgcacagt ttgtacaaaa
    10441 agttagaatt acaacaaaag aagggaagaa aatccccgct gtcataaatg ctacatataa
    10501 taatggtaag gctgaaaata tatatgcaga tattggagca tatacagaag aagatgtata
    10561 taacctaggg attaatgttg gggatatggt aacatataca acaagctttg aggaatttac
    10621 tttaccagac cgattagtgg gaaaggcttt tgatgatagg ataggatgtt ttgtgatggg
    10681 agaagtttta aaagaactta ggaaagaaaa tctaaattgt aatattcact ttgcggctac
    10741 aagtagtgaa gaggttggaa taaggggagc aaagacatca acacagttaa taaatccaga
    10801 tatagtattt gtaatagatg tagcatgtgc taaaaatgag tttgtgagag actatacaaa
    10861 taagaaacaa attgataaag gtgtaatgct aatgcataga gataggactc tagtccctag
    10921 tagaaaaatg atagattatg taatggaaat tgctgataaa aataatatac cacttcaaca
    10981 tgatatgttt gaatcaggtg ggactgatgg aggagaagca catctcgtaa atgaagggaa
    11041 accttgtgta gtaacttgtg taccagttag atatggtcat tgtgcatttt caatagtaag
    11101 taataaagat ttggagaaca ttataagatt gtatacacag ttaattttaa attttgatga
    11161 agaacaatat aaacatataa gaaattttat ctaaaggaga ataagaaaat gtcaacagaa
    11221 gtacaaataa tggaacttat atcaactgca ggagagagta aatcgaaagc ttttgaggct
    11281 ttaaaaaaag tgaagtcaaa agattttaaa ggagcaaaat tgttaataga ggaggctcgt
    11341 aaaatagata ttgaagctca caggatacaa acagaattaa taactcaaga tttaaatgaa
    11401 gatgttgaaa gtccagcaat gagtctttta atggtacatg ctcaagacca ctatatgact
    11461 tctcagctag caaaagactt gatagaggct ttgatatatt gtcttgataa gtaattgtaa
    11521 tatagaaata gattatttat gtaaaaattc ttttaaaagt ttagatgctt ttaagagata
    11581 ataaaaatac aaactttagg aggaattatt atgaaaatat tattatgttg tgcaggtgga
    11641 ttttctacta atatgttaat gcaaaatatg aaaaaggtgg taaaagagag tgctaaactc
    11701 aatgaaaaag attttgagtt tacagctata ccagcagatt caatagaaag tgaagttgat
    11761 gattgggata tagtacttat aggtcctcaa atagctcaca aaatagactt cttaaaagga
    11821 atattagagc caaaaaataa gccttttgca gtaatagata aagatgtata tggaaaaatg
    11881 gatggagcta cagttatgaa gttggcatta ataacttata agaaacatca attagctcaa
    11941 ggataataaa aataatgagg gaaaagggag attagatatg ttttcaaaat ttgaagcatt
    12001 tatgaataga gtatttatgc ctttagctca taaggttgac aatcaacgtc accttggttc
    12061 tataaaggct ggtatggttg ctatgacacc attcacaata ttaggaagta tatttgcaat
    12121 acttccagca cttccaaata tgcttggaga aaataatcca ataagcaaat tcatacttag
    12181 caattcagca atattggatt taccagtaag tctttccata ggtttaatag gattatatgc
    12241 ttgtatgtgt attgctttta atctggggaa ctactataaa ttatatatac caggttgtgt
    12301 tgcactatca actttttctt ttttattcct agtagcctca tttacaccag atggagcttt
    12361 agaaatagga aatttaggag ctaaagggct atttacaggt atgatagcag ggcttggttc
    12421 tgtggagtta tataattttt gtaataagaa gaatatcaca ataaaaatgc cagaaaatgt
    12481 acctgatttt gtatctaggt cttttgaatt aatacctact actataatag tttgtggaac
    12541 ttttatagtt gctcgtttta tttcattaaa agtatttgga gaattacctc cacagatact
    12601 tactcaattt ttatctccat tagtaggaag tatggataac ccatggtcag ttcttgcact
    12661 taactttgct atatgtacta tattcttttt tggaatacat tcatcagtat ttagtcctat
    12721 aactagacct ataatgatta catttatagc tgaaaacata gcagctatgc aggctggaga
    12781 aacaatacct catttcttta cagcaggagc ttcaagtgca ttctttggat ttacaggatg
    12841 tggaattagt ataggttgtg taattgcatg tatgttatca aaaaatgctt cctataagaa
    12901 gattggacgt gtatcattat tccctgcatt atttgggata aatgaaccaa tattatttgg
    12961 agcaccgata atattaaatc caataatgtt tataccacat gtatttggtg gagctataat
    13021 aggtacacta ccaatgtttt ttatgcactg gggatggtta gcaaagccag tatttgaccc
    13081 tccatatgta ggggtattct tagaaggatt tttaactaat ggagattggc attctatatt
    13141 agcaaatgca ttgcaattag tattagcagt tttattatat tggccattct ttaaaataat
    13201 ggaacgtcaa caaaataata aaaaacaaaa agaagaaact aagaatatat tctctaaaga
    13261 agaagaaggc ttattggatg atttaggatt ggatttctaa tattttgtta tgaataatac
    13321 aatggagaga ttagtatcct taatctataa aagaggtgtt gacgctcttt tgattaaagc
    13381 aaaaaatact aaaagatata taggagcttt atcaggtagt ggtgtatatg tgcttgttac
    13441 taaggataaa aattatcaaa ttctagatgg cagatatatt gatgaagcag ataaaaaaac
    13501 gactggtttc ataaaaagga ttgtatctca aggttcttat ataccaacta taattgagtt
    13561 attaaaagaa ttaaatataa aaaagttagc aatagaaaac caagctatga gtatacaaga
    13621 gtatctttca ctaaaaaatg aaggttttga aatcaattta ataacaaatg aattatggaa
    13681 gctaagggct ataaaatcta aagaagaaat taaacttatt aaaaaggctt gtgagattac
    13741 agatgaagta tttgatgagg ttatttctga aattaaaaaa gatatgactg agctagaagt
    13801 atcagctcta attcagtatc atgcattaaa aaaaggtgca agtggaatgt cttttgaaac
    13861 aatagtggta tcaggagaaa gaggtgcaat gccacatgga agacctacaa acaaaaaatt
    13921 gaaagtgaat gaagctataa caatagattt tggtgttgtt tatcaagggt atcaatcaga
    13981 catgactcgt acaatatcta taggcaaacc tcctaaaatt attaaagaga tatatgatgt
    14041 agtattggaa gcacaattat ctgcaataga gtcaataaag gaaggaacta gggcaagtga
    14101 tgttgacaaa gtggctagaa aaattataga taaacatgga tttggcgaat attttaatca
    14161 tggactgggt catggtatag gtcttggaga tggtgaagtt cccactttaa atccaaatag
    14221 tgaagatata ttagtggaag gaatggtaat gtcttgtgag ccaggtattt atattccaaa
    14281 tgtaggggga gttagaatag aagatgatat tgttataata gatggaaaag gtgttccact
    14341 caataagaca tccaaagagt ttataatatt aggagagtaa aattatgaaa tataatttta
    14401 ataaagtatt aaatcgtaaa gaaggaaatt gtagaaaatg gagcaaccat gtgattaaag
    14461 aaaaatttgg cttaaatgaa gatgcaattc ctatggattt agctgatatc gattttgagt
    14521 gtgctcctgc tataaaagaa tctatcttaa aaagagcatc agtaggagat tatagttata
    14581 cttttatagg agatgacttc tatgatgcta ttataaattg gaataaaaga agatttgatg
    14641 tatatataga aaaggattgg ataaaattaa cttttggtac tgtttcaacc cttcattata
    14701 tagtccaagc atatactaaa gaaggagagg gtgtattgat aaatacacct gcttatgacc
    14761 cttttgcaga ggctgtagta aataataata gaaagttgtg ttgtagctca ttaaaattag
    14821 ataacaatag atattattta gatttagagg atatggaaaa tcaaatgaaa tacgagaata
    14881 taagattgct tatattctgt agtccacaaa atccatcagg tcgtatatgg acaaaagaag
    14941 aactatatca agtgtcagag ttatgtttaa agtataatgt aattttggta agtgatgaaa
    15001 ttcatagaga tataatattt aaagattata gatttgtatc tttgtggaat gctcatccag
    15061 aaatttataa aaatagtata atctgtgtat ctcctaataa aggcttcaac ttaggtggat
    15121 taaaaacatc atatgtatta ataagagatg taaaaattag ggagacttta ttagaaaggc
    15181 taaaaagtaa ttcaataact tctccaaatg tatttgcaat accagcaata gttgcagcat
    15241 ataatgaaag cgaagaatgg ctagatgcta tgactagtta tgtagaggag aattttgaaa
    15301 tagtttatga tttctttgaa actaatatac caaaagctaa agtgatgaag tcagactctt
    15361 cttttttagc ctggatagat gtaagagaag tatttagaaa tgaagaagaa tctaaagaat
    15421 tttttagaca tgcaaactta actatggtag ttggaagtta ttttgtgaaa gatggagatg
    15481 gatttattcg tattaatgtg gggtgtccta gagaaacatt aaatgaggct ctaaatagaa
    15541 tcaaaaaaac gtatataagt atgtattgtt aaatcataaa aaaagtatct atggtttata
    15601 taaaaacaca ttatttagtt atcaaatatt actggaattt tgtattccag taatattttt
    15661 tgattaagga atattattga ggaatattaa ataatattat ttcataccct tatattaact
    15721 tatataaaaa tattttatta ttttgggaac gggttagcta acctttatct agtgtatatt
    15781 tatattgtaa gaagcagtaa aaacttttcc tttactaagt ttattaataa acttcttatc
    15841 tttataaatt ttgttagaat ttatactatc agcaagacca taccaaggtt ctatacataa
    15901 aaaaggagca gtagaattag tttcactata ataaggagtc caaataccaa ctagagggaa
    15961 atcttccata gatatagtta catatttact accatcatta ttacatatag acacttcatc
    16021 tatgttggtg tatatcaaag catcattctt gaatacttct ggatttaact gtaagttttt
    16081 taattttcca ataggtatta tatcatctgt aaaagaacca tttaaattga ttttttctac
    16141 atcattttta cttttaaact caaggtagtg tttagaaaaa tcattttgtt cgtaaaaagg
    16201 aaggttgaat gcaggatgtc cacctataga aaagaatata tctttagaat cagtattttt
    16261 tacaatccat tctacctcaa tactattatc atttaatgta tagtttatga ttagctcaaa
    16321 agaatatgga tacttcttta aagtagattc attatctgtt aatttataag ttacaaatgt
    16381 atctccttta tctacaatct caaagtccat atctctagca aacccatgtt gagtcatgct
    16441 atataaattt tcttctatta ttgtttcatt atcaaaaagt ttacctacta tagggaacaa
    16501 tataggtgat tgtctacccc aatatttttt attaccatcc caaagaatgt ttttgtcgta
    16561 ttttttagaa aatattttag ttagttcagc accaaaactg cttgattcga taattaaatt
    16621 ttcattttgt aatatattca tttaaaatct cctttattat attttatgag tatattatac
    16681 tataaagtat aaagattatt aaattttatt tgatattata tttgaaaggt agattttagt
    16741 atattttgaa aattttgttt tgaaaatttg ggattattta agaaattttg tattataaat
    16801 agaaggaaat agataaattg tatagaataa aatatattaa gataagttaa taatgttaaa
    16861 tgggaagtga gtttatggaa aaaccaaata caaaagttgt taactttcaa gagtataaag
    16921 acagaaaaaa ccaaagagaa gtaaatagag acgaactttt aaagcttata aaaaaaattg
    16981 tgacttcaaa atagggagtt gttaaactcc ctattttaat gggtttcata ataattaaga
    17041 cattctaagt ccatctatat aatctttgaa ttgtgagaaa tctgattcac tacttaattc
    17101 tatggtttct gaaccttccg tataaaaatt cttaagatag atagtataat tattatcaac
    17161 acaatcatga taaatacatc tatatccatc ttcatacaaa ctttttaact tagaaaaatc
    17221 atccacaata tcatctcctt aaagttcttt taaatatatt tttacaaaaa gcatctaatt
    17281 gtatgcaaat tgacaatgta aaataaagga aatgaacata aaatgtagaa taatttttat
    17341 atgtatgtaa aaataaaaag aaaaagttga aatttttaga aaataagagt attttttatc
    17401 taaaaagaga gtttaaaaaa taaaaaaatg ttgggaggat aaaaaatgag tttattagaa
    17461 agtatatcaa aaaacatata ttccctagat aactcttcta ttgaaaaaac aaagcagaga
    17521 ttggataggc ttatacatcc aactggaagc ttaggaaaga tagaagatat ttgcatgcaa
    17581 ttagcaggaa tatttggcaa tgaaaatttt gacacaagta aaaaggttat aattgctttt
    17641 gcgggagacc atggtgtata tgaagaaggc gtagcacctg accctcaaaa tataacaaaa
    17701 ttacagtttc caaatttttc gaaaggtcta tgcggagttg gagtaataag taaatttgta
    17761 ggggcagatg ttgtagctgt agatgtagga ataaactgtg atgaaaaact ggatggggta
    17821 ttagactaca agataagaaa agggacatca aatatggcaa aaggtccagc tatgagtaaa
    17881 caagaagcaa ttagatgttt agagataggt attgaaatag cagagcagtg tatagaaaaa
    17941 gattataagg ttattggtat tggggagatg ggtatagcaa atactactcc aagtacagca
    18001 ataatatctg taatttcagg gtgtgacccg ttagaagtta ctggtatagg tgcaggtctt
    18061 aagaaagaaa gactaaagca taaggctgaa gtaattagaa aggctattga aattaataat
    18121 ccaaatccaa ctgatggagt agatatttta tctaaagtag gagggtttga aataggctca
    18181 atggcaggtg ttatattagg ttgtagtgct aataggattc cagtagtaat tgatgggttt
    18241 atttcttatg cagcagcatt aattgcatat aagataaatc caaaaactag agaatatatg
    18301 atagcatctc atttatcagc agaatcagga actaaaagag cattagacat attaaaatta
    18361 gaccctctat taaatatgga tatgagatta ggtgagggaa gtggagctgc tttagcattt
    18421 gatataatag aagcttcaaa ctatacttat aaaaatatgg caacttttga tgaaattgac
    18481 atgggaagat aaatataaaa atttaatatg taggagtgtt ttgatgagta aaattattct
    18541 agtaacagga ggagctaggt cagggaagag taactttgca gaaaaactat gttttgacag
    18601 aaataataat acagcttata tagcaacctc tattccattt gatgatgaaa tgaaagatag
    18661 agtaaaaaaa catcaagaga atagacctag aaactggaat acttatgaaa tatatgaaga
    18721 tatatattcc ataattaagg agatatataa agaacatgaa actgttatac tagattgtgt
    18781 aacattatta gtaaataatc taatgtttga acacaatcta aatatagaag aatctagtca
    18841 agaagaaata aacaaattag aacaatacat aaaagaacaa gttgtcaaac ttattgaaga
    18901 aatagaaaaa acaaatctat actttgtatt tgtaacgaat gagttaggta tgggaattgt
    18961 tcctgggaat aaacttagta gaatttatgc tgatattgta ggtcgtataa atcaatatat
    19021 agcttcgaaa agtaatgaag tttattttgt agttagtggc atagctatga agataaagga
    19081 gtagtatgca attatgaaaa gatttatttt aatactacag tttttgacaa ggatacctat
    19141 aaaattaaat gtaggatttg atgatgaatt ttataagtca atagtatatt ttccacttgt
    19201 aggttttgtc ataggtatat tatcctattt aataggatgg atttctatgc ttttatttga
    19261 accatttata gcttctataa taataactct agctggtgtc ttaattactg gaggacttca
    19321 tatagatgga ttaggagata catttgatgc aatatatagt tatagggata aagaaaagat
    19381 gcttgaaata atgaaagatt caagattagg tacaaattca cttctagcaa ttatgtttgt
    19441 acttttatta aaggttggat tcgtttacga tataataagt aataattcat tgtgggttat
    19501 aatttttatg ccaatgattg caaggttagg agtaatgtta ttgacatata aaactgtgac
    19561 accaagagaa aaaggaatgg gaaatttatt tataggaaaa ttaactacaa gtatgttaat
    19621 aactgctata atatacacat tattgatagt tgctcttatt actaaattta tatttttatt
    19681 acctaatata gtgttgatta aagtattagg ttcgattata gtggtttttg tatttataat
    19741 attattcaaa aaacatattt ataaaaaaat tgacggagta acaggagata tattaggctg
    19801 tggaatagaa ctttcagagc ttgtatattt aatttacata tatttgttaa tttttatgtt
    19861 tttttaaagt aaaaagtggt gataatatga taagattaat attaataaga catgcactga
    19921 caaatgataa taaaaaagga agattatcag gccatataaa tagttgtatt agtgaagagg
    19981 gcaaactaca aataaataaa attaccagat atctgagtaa tgaaaacata gataaggttt
    20041 atacaacacc atctacacgt actaaagata cagtagaaaa aatatcaaaa ttgaaattat
    20101 tagaaataga agaaaaagaa gctttaagag aaattagttt tggagacttt gaaggaagaa
    20161 cttttgaaga aattaaaatc aaatatccaa atgagtttga aaaaatgata aaagaaggta
    20221 acaactatag atatccaaat ggagaaagtt tgatagactc ttataaaaga gtggcaaaag
    20281 aaattgataa tataatttta gaaaataata gtaatttaga tacaaagaca atacttatat
    20341 gttctcatgc aggaacaata agaaatataa taacacattt gatttctggt agttataagt
    20401 atcactggaa ctttaaaatt gataatgcat ctattacagt tttagaaatt gatggtggat
    20461 ttgctgtaat agataaaatg aattttacag acttcatata atttaatcaa aaccaaacat
    20521 tacaatctgt gtttgtaata tatctgtaaa attagcaatg aaaatagaat attagattaa
    20581 aattaggttc ttaattagat gttaaaaggg aaaaaggtta gatgcctttg cagcccccgc
    20641 tactgtgaaa ccaacgaaac tataaatacc actgtcagtt tgatgggaag gttatagaag
    20701 taggatgaag ttaagccagg atacctgcct aatttaattt aaaacaagag gtcttcgggg
    20761 tgaaagattt ttaattaata tatataaaag tcctatcttt agaagtatta ggactttttt
    20821 taatttgttt tttagtaaat tttaacaagg agggatggaa agtggggaaa aaaataatgc
    20881 tacaaggaac agcttcaaat gtaggtaaaa gtgtcttagt agcaggtttg tgtagaatat
    20941 ttaagcaaga tggttatact gttgctcctt ttaagtcaca aaatatggct ttaaattctt
    21001 ttataactaa agaaggatta gaaatgggaa gagcacaagt atttcaagca gaggctgcga
    21061 aaatagagcc aatagctgat atgaatccag ttttattaaa accttctgga aataataaaa
    21121 gtcaagttat tgtaagaggt aaagttgtag gagaaatgcc ttcttctaag tatcatgatt
    21181 ataaattgga gttagtagat gtgttgaagg aaacatttaa tagcttaaat tcaaggtatg
    21241 atattgtagt tatggaaggg gcaggaagta atgcagagat aaacttaaaa gatagagata
    21301 tctctaatat gggtatggca gaaatagctg atgcacctgt aataatagtc ggagatattg
    21361 atagaggagg cgtatttgcc tcattagcag gaacaatgct tttattaaat gaagatgaga
    21421 aaagaagagt aaaaggtgta ataatcaata aattcagagg aaaaaaagag cttttacaag
    21481 atggtgtaaa gatgctagaa gacatcataa aagtacctgt tataggagta gtaccttata
    21541 cagatataaa aatagaagat gaagacagtg taactacgag atttaagaaa aaaatgaata
    21601 aaagtgatat acacatagaa ataattagaa ccccacatat gtcaaatttt acagatttta
    21661 atatatttga gacacaagaa gatgtaagta taagatatgt agactatgga gaatctttag
    21721 gaaatccaga tatattaata atacctggaa ctaaaagtac tatagacgat ttaacatata
    21781 taagaaaaag tggtcttgaa tcgcaaatca aaaatttaca tagtcaaggt aaattgatat
    21841 ttgggatatg tggtggatac caaatgttag gtaaaaaatt aaaagaccct tatcatgttg
    21901 aaagtgaaat tgaagaagtt gatggaatag gtcttttaga tacagaaaca acgtttgaga
    21961 aagaaaagac tacaacacaa gtagaagcta ctattgttga atgtatcagt gaatacatga
    22021 ataaccttaa gggaaaaaaa gtaaaaggtt atgaaattca tatgggaatt acaaagcgag
    22081 gtaatgatgt aagaagttta gatttaataa ctaaaaaact tgataaagaa gtgaattata
    22141 ctgagggaag tgtaaataaa gaggggaatg taattgggac ttatcttcat ggaatatttg
    22201 atgaaataag ctttacaaga gcaattttaa acaatataag aaagaaaaaa ggtttaggag
    22261 aattagaaag caaagtaaac tcttttaatg aatttaagga taaagagtat gataagttgg
    22321 ctgatttttt aagagaacat ttagatatag agaaaatata tgagattatg aaataatgat
    22381 gacctttatt tataatttag gtaggaggtg tattatgaaa aaaattttaa ttgcgggtac
    22441 taatagtgga gtaggtaaaa caacaatatc attaggaata atgcaagctt tggttaagag
    22501 aaatatgaaa gtacagccat ataaagtagg gccagactat atagaccctt catatcacac
    22561 ttttataact ggaagacatt ctagaaattt agattcttat atgttagatg atgaaaaaat
    22621 aaagtatata gtaaataagt cctcaaaaga tgcagatatt tcagtaatag aaggtgttat
    22681 gggcttatat gatggctttg gaatagattt agataattgt acaagttcac atacttcaaa
    22741 ggtattaaag tcacctgtta tattagttat aaatggtaaa tctatggcag catctagtgc
    22801 tgctatggta cttggatata aggaactaga taaagatgta aatataaagg gtgttatagt
    22861 aaataatgta aagactaata gccattatca aataattaag agttctatag aaaaatattg
    22921 taatgttgaa gtattggggt attttcctcc aaataacaaa ttctctttgg aatctcgaca
    22981 tctaggatta gttcctagtg tagaaatgaa atcactaagg gaaaaatttt ataccttagc
    23041 agatgaaatt gaacactata tagatattga cagaataatt gaaatatcag aaagtgatga
    23101 atttgagagt gattttgatt ttcaaacttt gatagaaaat aataaaataa aagaacatgt
    23161 taataataaa tcaatagcta tagcatatga taaagcattc aatttttatt atagagaaaa
    23221 tatagagctt ttagaggaac ttggtttaga aataaactat tttagccctc ttgaagatgt
    23281 atcagtgcca aatgcagatt atatttatat tggaggaggt tttccagaaa tatttggaaa
    23341 agaattagaa gcaaatgagt caatgagaac ttccatatta gaagcacata acaataatat
    23401 acctatttat gcagaatgtg gaggactgat gtacctaggt gagaaactat tgaatcaaga
    23461 agaacaagaa tttaatatgg ttggaatatt tagtggaatt agtaagatga catcttcttt
    23521 aaagagattt gggtactgta ttggtgtagc caaggaaaat actcttttat ctaaaaaagg
    23581 ggaatccatt aaagggcatg agtttcatca ttcaatattt gaaagtgatg aaaaatgtgt
    23641 atattcaatg agaaaagaaa gagatggaaa tatagtagaa gagtgggatg gtggttatag
    23701 taaaggaaac actttggcta catatcttca tactcatttt tataataact taaattgtat
    23761 agaaaatttt ttgaaaaaag gaaatgaata aatgagtatt ttatctattt atataggata
    23821 tgtgacagac ttaattgtag gagacccata ttcttttcct catccagtga gatttattgg
    23881 taaacttata aattttactc aaggtataat aagaaaggtc tctaaaaatg ataggcagtt
    23941 aaaatttggg ggatttatat tatggtttat aaccgttgga ataacatatt taacaactta
    24001 tgctatagtg aagttattta gttttaatat attattgagt attgtagtga atagttttat
    24061 aatttataca acactagcca caaaatgttt aaaagacgaa gcattaaaaa tttataatgt
    24121 attaaaaact gaagatatag aaaaatctag gattcagcta tcttatatag taggtaggga
    24181 tactactaat ttaagtgaac ctgaaataat aagagcaact gtagaaactg ttgcagaaaa
    24241 tactgtagat ggtataattg cgcctatgtt ttatgcattt attggaggag caccacttgc
    24301 tatggcctat aaagctatca atacattgga ctcaacagtt ggatataaaa ataaaaagta
    24361 taaagatatt ggttttgcat ctgctaagat tgatgatata gccaattata taccagctag
    24421 aatatctgtc attcttatga caattggaag tttcttttta aattataact ataggaactg
    24481 ttttaaaatc tctattagag atagaaagaa tcataaaagt ccaaattgtg ctttttcaga
    24541 aggtgcagta tctggtgctc taggaataca actaggaggt accaacgttt attttgggga
    24601 aaaagtatat aagccaacaa taggggataa gcttagggag atagataaag aagatataat
    24661 taaaactaat aaaattatgt atgcatcttc atttgtatct atgttggtat ttacactaat
    24721 atatgtgtta tgtaggatga ttttaattaa cttaattata taaattattg tttgttaact
    24781 taagggggac atagatgaag gatttagggc atggtgccaa tgtagatgaa atggcaagat
    24841 tatatgggaa aaacccaaaa gaaataattg attttagttc aaacataaat ccaaatgtat
    24901 taccaaattt agagagatac attttaaaag gattagaaga gtgtaggaat tatccagata
    24961 taaattatac aaacttgaga gaaaatatat caaaatatat agatataaat ccagacttta
    25021 taatacctgg caatggtgca acagaagtga tttatttgct tatgaagagt attaaaaaaa
    25081 aattggctat aataaatccg actttttcag agtatagaag aagtgctgag ttaaataatt
    25141 tagatattat agatttagag ttagatttag aaaataactt taaacttaat atagatataa
    25201 taaaagaaaa tataaaaaga tttgatagtt tatttatatg taatcctaat aatccgagtg
    25261 gtaatgtcca agatttaaag gaacttgttc atttactaga taaacataat aaggtactaa
    25321 taattgatga aactttcatg gaatttgttg aagatgaaag taaatatagt ttggttaaat
    25381 atatagaatc aaataaaaat atatttataa ttaaagctgt gactaagttt tttggtatgc
    25441 ctggtttaag gctaggatat ggtcttacaa gcaacactga aattatgaac aaaatttatg
    25501 aacacaaaga gccatggact atcaattctt ttgcagatat actatccaat tttatttttg
    25561 aagataaaga gtatattaaa aatagtaaag aatattacat agaagaaaga aaatatatgt
    25621 tacaagagtt gagaaacata agaaatataa aggtctatga tacagatgct aattttatct
    25681 tgataagaat atataaaaaa acaactaaag aattgaaaaa agatttgttt aagcaaggaa
    25741 acatattagt tagagatgca tctaatttta taggattaga tgatagcttt ataagagtgg
    25801 ctataaaatc tcatgaagat aacaaaattc ttatagaaaa tatgaaaaat ttattgggtg
    25861 attaaaatga aatcttatgg aatatgccca gcatcttgtg gagaatttgt acaaggtata
    25921 attgatgatg aagaatattt atgttcatat gcaatagata tgtattctaa agtgtatatt
    25981 gaagaaaaat tagtagatat aaatttaggg agatataagt caaggttagc aattgaaaag
    26041 gtatttgaaa aatttaatct acctaaaaaa tatactaaaa atatttcttt aaatataaat
    26101 agtaaaatac ctgttggaaa aggaatggca agttcaactg ctgatatagg agctactata
    26161 aaggcaacat tatcattgat agataaagat ttaagtagcg aggagatttc taaattagca
    26221 gcagaaatag aaccaacaga ttctatattt atagataaaa atagtatatt taatcctcta
    26281 aatggaactg ttataaaata cctaggaaat ctgaccaatg cgaaagttgt aatacttgaa
    26341 ccaaacaaag tactagatac tatgaaaatt agattgaggc aagattataa taaattaaaa
    26401 gtagagaata aagaagtaat aaaaaaatca tttgctcttt tagaagaagg attgaaaaaa
    26461 aataatttat ctctagttgg agaggcttgt actttgagta gcttagcaaa tgagaatata
    26521 gaaaaaaaag agtatttaaa tgaaataata aaaatatcaa aaaaatatgg agcatatgga
    26581 gtaaatattg ctcacagtgg aactgtagta ggtattttaa ttgataagtt tatgaatgat
    26641 aaaaagatga tagatgcctt atgtgaatct aacataaatt ctgtctataa taaaatttat
    26701 acacaaaaca taattaatgg aggaattaag ggggaaatag aatggaatac ataaagaatc
    26761 ctatgaaaat agaagaaaaa agttttgaat tgatacaaga aataatagac gaaattagac
    26821 cagattataa atttaagaat aatatcgaag aaaaaataat aaaaagagct atacatacta
    26881 ctgctgattt tgattactta gatatattaa aaatatcaga agaagcagtt gatagtataa
    26941 tagatgcatt aaaaaataat gcaagtattt atactgatac aaatatggct cttagtggta
    27001 taaataaaag aaagttagaa tcattaggat gtaagtataa atgtttagtg gcagatgatg
    27061 aaactgtaaa attagctaaa gaaaaaggaa ttactcgttc tatggcagca gtagaaattg
    27121 cagctaaaga agaaggaaga aaaatattcg ttttaggtaa tgctccaaca gctttatata
    27181 aagttatgga gatgaaatca gaaggtagat tagatttaga tgcagttata ggagttcctg
    27241 taggttttgt aggagcacaa gaatcaaaag atgaagttga aaaaacagat ataccatata
    27301 taatttctaa aggtagaaaa ggtggcagta atttagcagc agcaatagta aatgctatat
    27361 tatatactat gtaggtacct atatggaaga atatgtatat atagatggaa aaaaatatag
    27421 aagaggatat actacaggtt catgtgcaac aggtgcatct aaggctgctg tatatatgtt
    27481 gattacaaaa aatagaatca atactataaa tatagatact cctaagggga taccattact
    27541 actaaaagtt gataatataa atatttctga tacatttgtg gaatgttcca taaaaaaaga
    27601 tggtggagat gatatagatg caactcatac catggatatt tatgcaagag ctgaaattgt
    27661 agctaaaaat gataagaaca aagggtatct tactctaaag gatatagata atttaagcac
    27721 caatagtgag tgtaaaagtg agctctataa gtttattagg gtttatggcg gaactggtat
    27781 tggtgtggtt acaaaaaaag gtcttagcgt ggatgttggt aaacctgcta taaatccgac
    27841 tccacttaaa atgataaatc atgaaataag aaagcttata ggagataatt ttgaatctat
    27901 acttggaaat gataaagttt taaaaataac aatctttgct ccacaaggag aaactgtagc
    27961 aaaaaaaaca tttaatccaa gattaggtat agttggagga atatctataa taggtacaac
    28021 tgggattgtt gaacctatga gtgatgaggg ttggaagaag tctctatcta tagaacttca
    28081 gatgaaaaaa gaacaaggct tagataaaat aatattggtt ccaggaaatc atggagaaca
    28141 gtttataaga gaaaaattaa acttagatat taagtatgta gttagggtaa gtaattttat
    28201 aggctatatg attaaagaag ctcaaagaat aggatataag aaaatcttaa tggcaggtca
    28261 tataggtaaa tttataaaag tatctgctgg tatttttaat actcatagta aggtagcaga
    28321 tgctagaagt gagatattag tggcaaactt agctttaatg ggagctagat acgaattttt
    28381 aaataaaata aatcaatgcg taactactga agaagctgta gagctaataa ataatagtga
    28441 atatagagaa gtatataata tattaagtaa taaatgtagg gagagagtaa agcaatactt
    28501 gaatgaagat tcagatgata ttgatgtgga agtaatcata ttttcaatgg ataagtcttt
    28561 attaggtaaa tcggataata cagatgattt agtggaggtc tttatatgat aaacataata
    28621 ggtctaggac ctggtaactt agactatata acaaaaaaag gtgaaaattt aatttccact
    28681 tctgatgtat taattggtgg taaaagaaat ttagagtcta taaaaaattt tgaaggagaa
    28741 aaaatagttc tggattctaa tttaagagaa attatagagt acataaataa caataaagaa
    28801 aagcaaatat caataatagc atctggggac ccactaatat atgggatagg tagatatcta
    28861 tctaaaaata ttgataataa gatgttaaac atggtttctg ggattagttc attacagtac
    28921 atattttcaa aaatatatgt agatatgaat gatgtatata ttacaagtag tcatggtaaa
    28981 gtaccagatt ttgattacat attatcacat aaaaaagtat gtatggttac agattcaaag
    29041 ataggaccaa aacaaataag tagagaaata atagatagaa atctaaataa aattattgtt
    29101 gtaggagaaa atctgtcata tgacaatgaa aaaataacaa tagcaaaacc agaagagata
    29161 ataagaatgg acaattttga tatgaatgtt gtggtgattt tagatgagga atagtgaatt
    29221 tataacaggt aaagttccaa ttactaagga agaggttaga gcaatatcaa taagtaagtt
    29281 aaatttagta aatgctaaaa acctcataga cataggggct ggtacaggaa gtgtaagtgt
    29341 tgaagctgca tataattatc ctaatttaaa ggttatatct atagagaaaa atgatgatgc
    29401 tatagagctt atagagaaaa ataaaaaaaa attcaacttg aaaaatatag aagtgataaa
    29461 aggatatgct ccaattaatc tagggttaaa tgttaaagtt gagtctattt ttcttggggg
    29521 aacaggaaat aatctggaag aaataataga atggtctaaa aaaaatctcg ttacaggagg
    29581 aagacttgta gctaatttta taataataga tacatttaat caaactttaa aactgcttaa
    29641 aaagcatgga tttaaagaaa tagatgtatg tgttttgaat gtttctaaac tagagaaact
    29701 aggaaaaggt gaatatttta aaccactaaa tcctatatat ataatatctt gtgaaaaagg
    29761 ggaaaatgat gatgaataaa gtacattttg taggagcagg acctggagat aaagagctga
    29821 taacattaaa aggatacaaa ttattaagta atgcagatgt agtgatatat gcaggttcac
    29881 ttgttaatcc tgaacttttg gaatactgta aagaagattg ccaaatacat aatagtgcac
    29941 atatggattt acaagagata atagatgtta tgagagaagg tatagaaaat aataaatctg
    30001 ttgtaagact gcaaacaggt gatttttcta tatatggttc aattagagaa caagtggaag
    30061 atttaaataa attgaatata gattatgact gtactcctgg agtaagttct tttttagggg
    30121 cagcatcatc tttaggggtt gaatatacag ttccagaaat ttctcaaagt gtaattataa
    30181 caagaatgga aggcagaact ccggttccag agaaagaatc aatacaatca tatgctaagc
    30241 atcagacatc tatggtaata tttttatctg ttcaagaaat tgagaaagtt gtgtctaaat
    30301 tactggaagg tggatatcca aaagatactc ctatagcagt tatatacaaa gcaacttggg
    30361 cagatgaaaa aatagttaaa ggaactttaa gtgatatagc tgtaaaagtt aaagaaaaca
    30421 acataaataa gactgcttta ataatggttg ggagattttt aggagaagag tataataatt
    30481 caaagttata tgataaggat ttcaaacatg aatatagagg gtaatattgc tataatctgt
    30541 attacagaaa atggaaaaaa attagctcta aagataaatt ctctagtaaa taactgtgtt
    30601 atatatcaag taaaaaataa aaaaagtaat ttagttgttg aagaaacatg tgtgcataca
    30661 gttcaaaaga agttaagtga ttttgtagga gaaatattta gcaaatttga ttacatagta
    30721 tttataatgg ctacaggtat agttgtacga agtatagcac catacataat aagtaagttt
    30781 agtgaccctg caattttggt aatggatgaa aaaggcaaaa acgtaataag tttattgagt
    30841 ggtcatattg gaggggcaaa cagcttgaca cagtacataa gtagtcttat tgatgctaat
    30901 ccagtaataa caacagctac tgatataaat caaaaagctt ccttagatat gatagcaaaa
    30961 aaactaaatg cacatattga taattttaga gaaagtgtaa aagaagtaaa ttctatgcta
    31021 gtgaataata aatgtgttgg attatatgtt gatgaaggat atcaagttga tactagggga
    31081 tttaaactta tagaaagtaa ggatgagtta agagatagtt tttcaaacac atatatagaa
    31141 aaattagttg tagtaagcaa taaaataaat tgtattgaag atattgagaa tttaaaccta
    31201 gataataaat ttgaacattg ctatagtacc caaaaaataa taaaggtaat tccaagagat
    31261 atagtgatag gaattggctg tagaagaaac acagatagtg aactgatgta taattcactt
    31321 gaagagtttc ttaataaaca aaatatagat attagagcaa taaacacagt tggaagtata
    31381 gaactgaaaa aagacgaaaa agcaataatt gaattatcaa aaatattgaa ggtgccattt
    31441 aaaacatttt cagtagatga aatatctaag gttgataatc tttttgataa atctgatttt
    31501 gttaaaaaaa atgtgggtgt gtattcagta gcagaacctg tagcatatct tttgagcaaa
    31561 ggtaatttgt tagttgaaaa acataaatat aaaggaataa ctttttcagt ggggagaata
    31621 aaaatatgat atacgttgta ggaattggac caggaagtaa agatacaatg actttagaag
    31681 caataaaagc aatagaagat tctgaggcta ttgttggata taagacttat ataaaactaa
    31741 tagaagaatt tatacaaggt aaagaaataa tccaaaatgg tatgaggcaa gaagttgata
    31801 gatgtaaaca agcagtggag gaagcaaaaa aaggaaaaaa agtagcagtt gtaagcagtg
    31861 gtgatgctgg aatatatggg atggcaggtc ttattttaga attaatatct aaagaagatg
    31921 aagatataaa agtaaaggta attccaggcg ttactgcaag tattggtgca gcagctatac
    31981 ttggagctcc tattatgcat gatttctgtc atataagttt gagtgattta atgactccgt
    32041 gggaagtaat tgaaaaaaga ttgagattgg ctgctgaagc cgattttgta atttgtttgt
    32101 ataatccaag aagtaaggga agaagtgaac atctaagcaa agcattcaaa ataatgggtg
    32161 agtttaaagg tggagataca cctgttggag tggtaaaaga tgtaggtaga gaaaaagaag
    32221 aaaagtttgt atgtactttt gaaaatatgg actttgaaaa agtcgatatg actacaatgg
    32281 ttataatagg aaataagtct acctatatta atggtgaatt gatgataact ccaagaggtt
    32341 atacagtatg attttaattt taggaggaac ttcagatagt ttatctatat gtgataagat
    32401 aaatgaactg aaaaatcaac catatatcct ttcagttact acagattatg gaagggaact
    32461 tgcacaacaa catgcagaga atgtaatttt aggtaagctt ggaaaagaag atatgctaaa
    32521 atttataaaa gataataata tagtaaaaat aatagatgcc actcatcctt atgcagttga
    32581 agtatccaaa actgctatta cttgtgcaaa actattgaat atagattata taaggtttga
    32641 gagaaaatct ttaattgaag aaatagatta tgataataaa tttatagtag atacaattga
    32701 agaagcctgt gaaatagcaa ataaaatagg gacaaatata ttcataggaa caggaagtaa
    32761 aaatttgaac agatttatag aggaaataag tgataaaaat ttagttgtta gagttcttcc
    32821 aacgagtgaa gtcatattaa gttgtgaaaa attaggttta agtgcagata atatagtagc
    32881 tatgaaaggt ccatttagcc agtatataaa tgaagaaaca tataaacatt acaatataga
    32941 tttagtcata acaaaagaaa gtggaatagc tggtgggttc ttagagaagg taaatgcatg
    33001 taaaactttc aatataccag taataattat aaaaagagaa tcgattaact acgaaaatgt
    33061 atttaatgac ataaataaaa ttaaagacat acttgtgtaa tttttaggag gagaatatat
    33121 gaagaaagcc ttattagtag tgagttttgg tacaagctat catgaaacga gagaaaagac
    33181 aatagatata tgtgaagata aaataaaaaa ttcgttaaaa agccatgatt tttttagagc
    33241 ttatacatca aatatgataa taaataaaat aaaaaaaagg gatggaatag aaatagataa
    33301 tccaatacag gctttagata aaatatatga acaaggatat gatgaagtaa taatccaaac
    33361 acttcatatt atatgtggcg aagaatttaa caaattaaaa gaacaagtag atggatattc
    33421 ttctaaattc aaaaagattg tattaggaag acctttgctt acacatattg aagattatca
    33481 ggaagctgta gaggcaatta agcatcaaat tcctcatatg gaaagtaatg aagcagtagt
    33541 atttatggga catggaacac ttcatgaatc acattcagcg tatccagcat tggaatacat
    33601 gcttagggat aatggaataa atgcttatgt tggtacagtt gaagggtatc cagaaataga
    33661 acatgtaata agaagattaa aagaaggaaa tataaaaact gtaaatttga tgccatttat
    33721 gttagttgca ggagaccatg ctataaatga tatggcagga gatgaagaag attcttggaa
    33781 aacaatatta gaagaaaatg gatttaacgt aagagttcat ttaaaaggac taggagaaaa
    33841 ttcttatata caagataaat ttgttcgtca tgctgtaaaa tgtgcagaga atgcgaagtt
    33901 ctatggtata ggagcagggc caggagatgg ttctttactt acgataaaag cggttaatac
    33961 tttaaagaaa ctggatattc tatacactcc agaatcaaaa aagggtggag atagtctagc
    34021 tttatcaata gttagtgaat atttacctga atcagtggaa ataaagtcta ggcatttccc
    34081 aatgagtttt gatgggaatg aaaaaagttt agcttgggac aatgtggcac aagaaattat
    34141 atcagatgtt aatgatggta aaaatgtagg atttgttaca ttaggagacc ctatgattta
    34201 tagtacatat gtatatataa tgaaaagaat attaggagag gtagaagtcg aaactatacc
    34261 aggaatttca tcattttcca atatagcatc taatcagaat tttccattag tcatggataa
    34321 agaagcttta gtggtaattc cttgtactat ggaagatgat aagatagagt atgctttgca
    34381 aaattataat tcaatagtat tgatgaaagt atataaaaat tttaaagaaa taataaaaaa
    34441 attagaaaag tatgatttaa tagaacatgc tattttagta agtaattctt cccaagaaag
    34501 cgaaaatgtg tttacagatt taaaagaagc gcaccttgaa gataagatat cttatttttc
    34561 tactatacta gtaaataaag ataataaaat aaaataggtg aaagcaatat ttaaaggaga
    34621 cttaatatga atatagtggt tggaactagg ggtagtaatc tagcgcttat acaaacagaa
    34681 tgggtaataa atgaattaaa aaagaagtat ccagaaatta gttttgagat taaaatcata
    34741 aagacaaaag gagatttaat tcaaaatgtt tcactggata aaataggtga taaaggattg
    34801 tttgtcaaag aaatagagca acaattatta gatggaaaaa tagacatagc agtacatagt
    34861 atgaaagata tgccttctta tttagcagat ggactaaaat ttgctcatac gcctaagaga
    34921 gaagacccaa gagatgttct aatacttaga gaaggatata aaaatttaga tgatttgcct
    34981 catggagcag taataggaac aggaagtaaa agaaggaagt ttcaattatt gaaacaaaga
    35041 cctgatttaa atatagtaca agttagaggt aatgtggaaa ctaggatacg aaaaataaaa
    35101 gatgaaaaca tgcatggtat tgtgttggca gcatcaggaa taattagagc taatttacaa
    35161 gataaaataa gttcttattt acctgttgat gttgttattc cagcacctgc ccaaggagct
    35221 ttagctatag aaattagaag caatgatagt acgatagagg gaatagtaaa ttcactgaag
    35281 gatgagaata cagaaatcca aatattggct gaaagaggtt ttttagatgg tgtaaatgga
    35341 agttgtcata ttccaatggc agcttattgt gaaataatcc aagataaaat acatcttact
    35401 ggcttatatg gagattcaga aggaaaaaaa gtagtaatca agtctataga tggagatatt
    35461 tcttctccta gagagttagg tttaaagtta gctaaacttg tattgaagga gtatgagaat
    35521 tatgaagggt aaggtttact tagtaggggc aggaccaggt gattataaac tcatgacttt
    35581 aaaaggttta gagtgtataa gaaaatcgga tgtaatagta tatgatagat tagcaaatag
    35641 tagttattta agagaagcta agcctgattg tgaacttata tatgtaggca aggcatcaag
    35701 taatcatatt ttgccacaag aagatataaa tagaattata gcagaaaaag ctaaagaagg
    35761 aaaaattgta actcgtctta aaggaggaga cccttatgta tttggcagag ggggagaaga
    35821 agcagagact ttagtagatg aaggtataga atttgaagta gttccaggta taacttctgc
    35881 tataggaggt ttatgttatg cgggtattcc tataacacat agagaccatg cttcgtcatt
    35941 tcatgtaata acaggtcatt tgaagggtga tgatagtgga gaattaaact ggaatgctct
    36001 tgcaaataac aaaggtactc ttgtgttttt gatgggaatt tcaaacctag agaaaataag
    36061 tgaaaactta atgaaagaag gaaaagataa ggatacacct gttgctctta taagttgggc
    36121 aactagatat aatcaaagag ttgttacatc tacacttgaa aatgtttatg aaactgctat
    36181 taaagaagaa gtaaaaccgc caactcttat agtagttggg agtgttgtag gacttagaga
    36241 aaagttgaac ttctttgaaa gtaagccttt atttggaaaa agtattgctg taactaggtc
    36301 tagaaatcaa aatagtgtat tggtagaaaa aataatggat ttaggtggaa atcctataga
    36361 aatacctact attaaggtag aaaaaataca aaataatatt aacttagaaa atgaaataaa
    36421 aaatataaat aagtataatt atctaatact aactagtaaa aatgccgtag aaatcttctt
    36481 tgaaaagata tatgaaatga attttgattt aaggatatta tctaatttaa aggtatgtgc
    36541 tataggttct gctacatcaa atgagttgaa aaaaagagga attatagcgg atatagtacc
    36601 aaaaaaattt gtagcagagt cactgtatga agagttagcg cctattttaa atgatgacga
    36661 taatatactt atacctagag cagaaaatgc tagagattat ctggttgaca aactcaaggc
    36721 tatttgtgaa gtaacagaag tacatatata tagaacagta atagatgagg agaaaaaagg
    36781 tgagatatta gatattttaa attctaatga catagattat ataacattta ctagttcctc
    36841 aacagtaaat aatcttgttg aaattatagg aaaagaaaat attaaaatat tagaaaaaat
    36901 aaaaacacta tctataggtc ctataactac agagacaata aatagtttgg gaataaaatt
    36961 gtacaaagaa tctgctacat ctactataga ttcgttagta gatttaatta taaaaaatta
    37021 attttggagg cttgaatatg ttaagaagac caagaagact aagagcaagt aaagcaatca
    37081 gaaatttggt aagagaaact aaggtgaatg tagaagactt aatatatcca ctgtttgtag
    37141 tagaggggga aaatataaaa gaagaaatta aatctttacc agatgtctat catttatcaa
    37201 tagatatgct agaagacgaa ataaaagaaa taagtgagtt gggaattgaa catataatgt
    37261 tatttggtgt tccagatgaa aaagatgagt gtgcttcaca agcttttaat gataatggaa
    37321 tagttcaaaa ggcaattaga aaggtaaaag aaataaattc agatataaat gtaataacag
    37381 atgtctgtat gtgccaatat acaagtcatg gacattgtgg gattttaact gatactggtt
    37441 atgttgataa tgataaatca ttggagtatt tggctaaaat atctgtaagc catgctaaag
    37501 caggagctga tatggttgct ccatctgata tgatggatgg aagaatacaa gcaataagag
    37561 aagctttaga tgaaaatgga ttagaaaatg ttggaatcat ggcttatagt gttaagtatg
    37621 cttcaaatta ttatggacct tttagagagg cagctcattc agcaccatct tttggagata
    37681 gaaaaacata tcaaatggac ccagcaaata gcaatgaagc tataatagag actgaacttg
    37741 atatattgga aggtgcagat atacttatgg ttaaacctgc attatcatat ttagatatta
    37801 taagaagagt taaagataat tttgacttac ctctagcagc ttataatgta agtggagaat
    37861 actctatgct taagtttgct gtgaaggaag gtttattaaa tgaagatgta attcttgaat
    37921 caattacatc tataaaaaga gcaggagcag atataataat aacttatttt gctaaagaca
    37981 ttgctaaact gttgaaaaaa taaattctag cagtataaag aatatcaaaa agtataggaa
    38041 ttgtaattaa ctattataca gtagttttgt ttagacattt attgaagaat aatagaaact
    38101 aatttgcaat ttctatactt tatttatgta actaaataaa atttggaggt agaagatgct
    38161 atatcctatt aatttaaaat tagatgagtt agatgtagtt ataattggtg gtggagaagt
    38221 tgcatatagg aaatgtaaaa attttttaga gtttaacaaa aatgttacta tagtttctaa
    38281 acaaatttta aataaatttt atgatttaaa aggaaatata aaaataataa aagatgatta
    38341 taaagaaata tatataaaag atgcttctgt aataattgct gccacaaata atagagagtt
    38401 aaatatggaa ataggattat attgtaaagc aaaaaataag ctagtgaatg tagttgataa
    38461 tatagaaata tcgaacttta ctgttccatc atatataaaa agaggagatt tacttataag
    38521 tgtgtctact gggggcaaga gtccttcatt atcatcaaag ataaaaaaag aaattgaaga
    38581 gagatatact gaagattatg aggagtattt aaatgtatta ggagatataa gaaaaaaggt
    38641 tataaaaaaa tacgaagata aaagtaaaag aaaagatgtg cttaatatgt tggtaacact
    38701 tgacctagaa gaacttaaga agtttgatat ataaaaaata aaataaaaaa tgttaaaaag
    38761 gtgttgacgg at
//
LOCUS       DAMJVK010000032        38118 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.032, whole genome shotgun sequence.
ACCESSION   DAMJVK010000032 DAMJVK010000000
VERSION     DAMJVK010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 38118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38118
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.032"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(151..690)
                     /gene="scpB"
                     /locus_tag="PC414_001689"
     CDS             complement(151..690)
                     /gene="scpB"
                     /locus_tag="PC414_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HDF2464086.1"
                     /translation="MKREDIKYIIESVMFAYGEPISIKELNYIINKELSSKEIEIMLN
                     LLIEEYREQNRGIQIIKLENKYQMCTNKDYAEYIKKIIEPKKKKSLSQATLETLTIIA
                     YKQPITKVEIEDIRGVKCDKVLQTLFENELIREAGRLNKIGKPIIYKTTDEFLKLLNI
                     ESLEELPPIENYQEVATNE"
     gene            complement(692..1411)
                     /locus_tag="PC414_001690"
     CDS             complement(692..1411)
                     /locus_tag="PC414_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HDF2464087.1"
                     /translation="MKYNIQLQVYEGPLDLLYDLITKHKIDIKDISIIDITKQYLNYL
                     KMLDKMDLEITSEFITMASKLLEIKSKYLLYKQKDEEEDPRIELMEKLEEYRKFKVAS
                     QDIKENITYVNERFYRNKEEIIIDDNVDLEDISIEAIKNILPYIFKVKTSQIENANDE
                     KLDKIVRKKIISVEEKILYIRDIIKEKIEVTFTNIIKSYENDEIIATFLSILELIKEK
                     EIVVVQDIFFDDILIRKSSEC"
     gene            complement(1441..2037)
                     /locus_tag="PC414_001691"
     CDS             complement(1441..2037)
                     /locus_tag="PC414_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HDF2464088.1"
                     /translation="MLSMNIGEIVASLVGIAMAISIHEFGHAYSAHLLGDDTAKAYGR
                     MTLNPVRHVDPIGLLAMFILKIGWAKPVPVNPNNFKNYKIGNLIVSLAGVFCNVLTAI
                     LCVIINKYVHMYAINTIAEYVFLYSIGFAAFNLLPIPPLDGWGVISTFVPYKWNEYLY
                     KFESMSYIILLIALFTGMYRIILTPIYAVLLKIVYLFV"
     gene            2335..3171
                     /locus_tag="PC414_001692"
     CDS             2335..3171
                     /locus_tag="PC414_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2464089.1"
                     /translation="MRQEERTVCFDRELKIEAYRFKGIMQKFPNHFHEHYVIGFIEKG
                     QRYLSCKNKEYTTSTGDLLLFNPFDSHTCEQIDDKVLDYRCINIKPEIMKKTVFEITG
                     KNYLPKFNQPVIFRSELVPLLQELHYIIMEEELDFKKEELFFFLIEQLIEEHTEPNLQ
                     SNLENTNIEIQAVCDYLENNYAEHIVLDELSTIAGMNKYSLLRNFTKLKGITPYRYLE
                     NIRVNKAKKLLEKGVEPIDAAIQTGFVDQSHFTNFFKNFIGLTPKQYQNIFINDDSNN
                     FK"
     gene            3192..3614
                     /locus_tag="PC414_001693"
     CDS             3192..3614
                     /locus_tag="PC414_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2000 domain-containing protein"
                     /protein_id="HDF2464090.1"
                     /translation="METKNSKCVMVIDENLPMGIISNTAAIMGITLGKHAPETVGPDV
                     IDKTGNSHLGIIDIPVPILKGNKEIIKDLRKKLYTLEFNDLTVVDFSDVAQSCNLYEE
                     FTQKIASVPEDELQYFGIAIYGNKKKVNKLTGSMPLLR"
     gene            complement(3825..4988)
                     /locus_tag="PC414_001694"
     CDS             complement(3825..4988)
                     /locus_tag="PC414_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDF2464091.1"
                     /translation="MKKLASTALAILIALTPLSFSFANNKENADANQLNISSKSAILM
                     DVGSGQILYEKDAHKKLPPASVTKVMTMLLIVEALDSGKIKLDDEVQVSETASSMGGS
                     QIFLEPGETQKVDTLLKGIAVASANDACVAMAEHLAGSVEGFVDRMNAKAKELNMNDT
                     HFANTNGLPVANHYTSAHDIALMSRELLKHEMISKYLTTWMDKVVVGKKQVTVGLANT
                     NKLIKHYQGATGVKTGFTQEAKYCLSASAKRGNTHLVAATLGAETSPERFNDASSLLN
                     YGFANYESVKLCSKGDNIATLTLDKADENKVKLVAKEDLNALIKKGSSKEFEKKIEIV
                     KNPKMPIKKGTVLGKIKICKDKKVIGEVELINTKDINKASYLQMLQRIIDNMI"
     gene            complement(5042..5242)
                     /locus_tag="PC414_001695"
     CDS             complement(5042..5242)
                     /locus_tag="PC414_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protein"
                     /protein_id="HDF2464092.1"
                     /translation="MDDVSRQNAIKALKQTKMEIAGEYGMNYEDAFEIIENASNKGVL
                     EGYFKKLEKKKNLGQGISRHLE"
     gene            complement(5448..7124)
                     /locus_tag="PC414_001696"
     CDS             complement(5448..7124)
                     /locus_tag="PC414_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDF2464093.1"
                     /translation="MQLFKKNTNKIKVMALGGLNEVGKNMTVVEYKDEIIVIDAGLSF
                     PEDEMLGVDIVIPDITYLVKNRDKIKGIFITHGHEDHIGALPYILKKINVPVYGARLS
                     IGLIQVKLKEHKMNNVKLNVIGPRQVIKLDNMEVEFLKNNHSIPDAYSIAIHTDQGII
                     YHTGDFKIDLTPIDGDVMDMHRICELSKKGVLLMLADSTNAEKPGFTMSEKTVGVGLD
                     ELFAKGNGRRIIVATFASNIHRLQQIINTAEKFNRKVAISGRSMVNVVGVAKELGYLD
                     ISDDMLIDLNDICKYEDSELVIITTGSQGEPMSALARMAFSEHKKVEIKSGDLVIISA
                     HPIPGNEKLISRVINFLFEKGAEVVYSDIADIHVSGHACQEELKLIHALVRPKFFMPA
                     HGEYRMLKRHAEIAEQLGMDKENIFVMQTGDVLELDKNSAKVANRIQTGNILVDGLGV
                     GDVGNIVLRDRKHLSEDGLMIVVVTISKDEGKVLAGPDIISRGFVYVRESEDLMDGAK
                     DIIKNVLNECEEKNIKEWAYLKNNIKENLKEYLYQKTKRNPMILPIIMEV"
     gene            7341..8078
                     /locus_tag="PC414_001697"
     CDS             7341..8078
                     /locus_tag="PC414_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDF2464094.1"
                     /translation="MRGKTHCAIGILTAIQTSLIFKIPISLVDILVSATFAVLPDLDK
                     SNSMVSNFILKNNVSKYIYRIFIYAVNIIIFFISININDNFYLSAIITFVAIIIIEAK
                     LTHTFLRKVFLSLIFILLAICLYIIEVEIYFTIFCLMLSIFPWLKHRSFSHSIFATII
                     VYFLLKQIELITNINNLSFYGTIGYASHMFLGDLFTKQGIPIFYPLSEKKISLGFLTV
                     GGPFSNFIEKSFIFVLIGLIIFSILKL"
     gene            complement(8195..8653)
                     /locus_tag="PC414_001698"
     CDS             complement(8195..8653)
                     /locus_tag="PC414_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDF2464095.1"
                     /translation="MANTMDLLKDKLKETGFKITPQRRAIVEILLKHDHSHLSSEEIY
                     DLVRVDCPEIGLATVYRTMQLLDEIGLISKLNLDDGCIRYEISLHKEDCHNHHHLICK
                     NCGKIMEAKEDLLDNIEKEIQSLYKFKILDHDVKFYGLCDECNGVSDSEE"
     gene            complement(8935..9222)
                     /locus_tag="PC414_001699"
     CDS             complement(8935..9222)
                     /locus_tag="PC414_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HDF2464096.1"
                     /translation="MEENIINLIDENGVESQFEIILTLEAEGKEYAILMPLDDEEAEE
                     ALIFRIDEDEEGEILIPLESDEEYETVVAVYTAIMEEEGLNYDEDESNGLN"
     gene            complement(9300..9725)
                     /gene="ruvX"
                     /locus_tag="PC414_001700"
     CDS             complement(9300..9725)
                     /gene="ruvX"
                     /locus_tag="PC414_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDF2464097.1"
                     /translation="MLDGRIMGLDVGDKTIGVAVSDLMGLTAQGVKTIKRVGKKKDIE
                     ELKAIIKEKQVNKIVSGLPKNMNGTLGPQGEKVIKFCELVKAETGIDVEFWDERLSTV
                     AAERSLLEADVSRQKRKKVIDMLAAVIILQGYLDFKINS"
     gene            complement(9931..12102)
                     /locus_tag="PC414_001701"
     CDS             complement(9931..12102)
                     /locus_tag="PC414_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464098.1"
                     /translation="MRKKIMSLFAIVIMIFVSTASYGFAEEKGKVIFIDMNRTNLGSM
                     MNIPILKNEIEKRGYVALMNIRGDQGTDDRRSYASMGAGTRASVTTEDYINFQKVDQD
                     TAKAFEVATGQKAKEINDLGINRSINENLEYGEYGANLGSLGKTLSENGLKTSVIGNA
                     DIIENNQLVKNRNLCLVAMDQYGRIDSGNIDNINKKDSKMPFGISTDYDKLLDETKKS
                     YKNSDAIFVELGDTYRLDMYRLNLNDNTYDIMKKNIVKNINKYLEQVFSMVGENDTVY
                     IASAFPSDLDYKNKRRLSPIIKFSGEGKGLLESATTRKAGIVANLDVGVDILGTFGLE
                     NKEMVGRSYTLVNKEDNISYLKSLYERIVSISSIRSTIINGFVGVISASWVIAMFAIF
                     FRNYMPHKERVFKVLKEFIKLGIIMPLSFMLAPLMNFKTPISISLGIFATTLILYLLG
                     RKLFKDNDIKHMGFFATLTILVIVIDSVFGTYLMQNNIMSYDAVIGARYYGVGNEYEG
                     VTIASAVFGLAVLLHYKKVSKLLVVIFSLIILITSAYPSMGANVGGAISECVAYLLFI
                     MLIYDIKIDFKKAVLLAVSAVVVVFAFAALDILSGSESHLGMFVQRIFLNGPGEILQT
                     FGRKIQMNMQLAQTSVWVNILLVGIGVIAVLIFKPSRHFRKIMNNFPILFKGFIASMV
                     GCIITLLVNDSGIVAASTASIYILIPLIIISINMIIFEKNN"
     gene            complement(12112..12369)
                     /locus_tag="PC414_001702"
     CDS             complement(12112..12369)
                     /locus_tag="PC414_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HDF2464099.1"
                     /translation="MEKDLDYTMKFEGIPEDRMSVGDTIDFVYKALVEKGYNPINQII
                     GYLLSGDSSYITSHKNARAIIKKFERDEILEEVITHYLNRK"
     gene            complement(12394..15033)
                     /gene="alaS"
                     /locus_tag="PC414_001703"
     CDS             complement(12394..15033)
                     /gene="alaS"
                     /locus_tag="PC414_001703"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HDF2464100.1"
                     /translation="MEKMGLNEIRSKFLEFFESKGHYVANSYSLVPNNDKSLLLINSG
                     MAPLKNYFSGVEVPPSVRMCTSQKCIRTGDIENVGITARHATFFEMMGNFSFGDYFKR
                     ESIKWGWEFVTEWLNIPEDKIWVTVYEEDDDSYDIWAKEMNFPEERMVRLGKDDNFWE
                     IGTGPCGPCSEIYFDRGEEYGCDNPDCKPGCDCDRYLEFWNHVFTQFDRDEEGNYSLL
                     ENKNIDTGMGLERMGCIMQGVDTIFEVDTIKSILEAVEKLTGVKYGENPKNDISIRII
                     TDHIRAVTFLVSDGVLPSNEGRGYVLRRLLRRAARHGKLLGVKELFLQKLIDEVIKVN
                     DKAYPVLVEKESYIKKVVGIEEEKFNETIDQGTEILNSYIEVLKNEGKTVLSGQEAFK
                     LYDTYGFPIDLTKEILEEEHLSVDEEAFNEEMEKQKERARNARGNMDGESWKEDPLSK
                     LESTVDSTFNGYSEIYGEGTIEAIVKDDELVQSAEEGDKVSIVLDNTTFYPEGGGQVG
                     DCGLITNENLVLEVLNTKKGANNSIKHIGIIKSGRISNGDKVKTLVDRETRMSAARNH
                     SATHLLHKALREVLGEHVNQAGSLVTPERLRFDITHFEAISNEELKVIEEKVNNVILS
                     SLDIKCDIMNIKEAKEKGATALFGEKYGDEVRVVSMGDYSTELCGGTHLTNTSQVGMF
                     KILSEGGVAAGVRRIEAITGKAVYEYLKERDGIISEVCVNLKSKEDNLIQRISSLLEE
                     NKNLSKELHDMKAKMSLQSVDSIFDSKVEVNGVNLITNKFEGMDMDTLRETADNLRDK
                     LGSGVVVLANVVDDKVNFVVTATKDVLDKGIHSGNIVREVAKIAGGKGGGRPNMAQAG
                     ASDVSKVDQALSYASEVIKTQVK"
     misc_binding    complement(15101..15339)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(15482..16561)
                     /gene="mnmA"
                     /locus_tag="PC414_001704"
     CDS             complement(15482..16561)
                     /gene="mnmA"
                     /locus_tag="PC414_001704"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HDF2464101.1"
                     /translation="MNKKVMIGMSGGVDSSVAAYLLKQQGYDVIGVTMKLWQDDDVVE
                     IEGGCCSLSAVEDARRVANKIGIPFYVLNFREVFKEKVIDYFIDEYLEGKTPNPCIAC
                     NKHIKFDDFYKKARQIGCDYVATGHYAKIEKDESTGRYLLKKSVTDKKDQTYALYNLT
                     QEQLEHTLLPIGDYEKDRVREIAKEMGMAVHNKPDSQEICFVKDNDYANYVKKHSKKR
                     IEEGFFVDTKGNILGKHKGILYYTIGQRKGLGITFGKPMFVIDINPINNTIVLGDNED
                     LFKKELIAKDVNFISIDTLEEPLRVQAKIRYSAKPSPATIHRVGEDTIKIVFDEAQRA
                     ITKGQSVVMYDGDIVVGGGIIEKSL"
     gene            complement(16850..17290)
                     /gene="nifU"
                     /locus_tag="PC414_001705"
     CDS             complement(16850..17290)
                     /gene="nifU"
                     /locus_tag="PC414_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HDF2464102.1"
                     /translation="MQYSDKVMEHFMNPRNMGEIDNASGVGEVGNPTCGDIMKIFLDI
                     DGDVIKDVKFKTFGCGSAIASSSMATEMIKGKTIKDALELTNKAVAEALDGLPPVKMH
                     CSVLAEQAVKAALIDYAQKNNIHIPELDGYVIDDAHDHDVEEEE"
     gene            complement(17344..18537)
                     /gene="nifS"
                     /locus_tag="PC414_001706"
     CDS             complement(17344..18537)
                     /gene="nifS"
                     /locus_tag="PC414_001706"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HDF2464103.1"
                     /translation="MEKRRLYMDYSATTPIKKEVLDAMMPYLTDYFGNASSFHTFGRE
                     AKDALDKAREQVAALINAEPSEIYFTAGGSESDNWTLEGVAYANKNKGNHIITSKIEH
                     HAILHTCEYLAKHHGFEITYLDVDSEGKVDLKQLEDSIKDTTILISIMFANNEIGTIQ
                     PIKEISEIAKKHKILFHTDAVQATGNIPVDVKELGIDLMSMSSHKIYGPKGVGALYIR
                     KGVRLHNFVHGGAQEKSKRAGTENIPAIVGYGKAAELAKENMQNHVETLTRLRNKLID
                     GVLERIPYTRVNGSLENRLPGNANFAFQFIEGEGILLLLDMLGIAGSSGSACTSGSLD
                     PSHVLLAIGLPHEIAHGSLRLTVGDFTTDDDIDYILENLPKVIERLRSMSPLYDDAKK
                     QGLVK"
     gene            complement(18555..19001)
                     /locus_tag="PC414_001707"
     CDS             complement(18555..19001)
                     /locus_tag="PC414_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDF2464104.1"
                     /translation="MKLSTKGRYGLKAMFELALNQDNGPVSLKFIAKKQKISDQYLEQ
                     IFSSLKKSGLVKSVRGAQGGYLLSKNAEDITVGDILVVLEGPVALSDCVLDEDVCENS
                     NMCVTKIVWEKMKKGIEDVIDSITLKDMINDYNKNKLENDITNIKK"
     gene            complement(19110..19880)
                     /locus_tag="PC414_001708"
     CDS             complement(19110..19880)
                     /locus_tag="PC414_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2464105.1"
                     /translation="MIIKLNSTYHSRVMKYLKKEPEYNLFIIGDIERYGYGNNFLNIW
                     ADVGEHGEIKAILLKYFEFMMFYSDGEYDVEGFYNLLRNTNYEEISGKICAVDALAKR
                     LGLNNLKVVDFCKLQTKKFLIDNNCNAKVKRIRLGNLKKTVKLYDLIDEFHSTTLENL
                     KNGLRTGRGYCIEINKQVVSMAKSTSENRTHAMIIGVGTHPKYRAKGLATKCLIKLCS
                     ELLRENKIPCLFYDNEEAGKIYKKLGFENIGKWGIYSK"
     gene            complement(19911..21176)
                     /locus_tag="PC414_001709"
     CDS             complement(19911..21176)
                     /locus_tag="PC414_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HDF2464106.1"
                     /translation="MPLADKIRPKTMNDVIGQSHIIGNGKILSKILQSNFLPNMIFFG
                     PPGVGKTTVAEIIAERSNKSFYKINATNSSLEDIKKVISELGSINNVNGVLLYIDEIQ
                     SFNKKQQQSILEFMENGSITLIASTTENPYHYVYKALLSRSTVFEFKPLEKLDIEKGL
                     KRAVEVLNEDSYMDIECNNDAIEDIAILSDGDMRRALNILEVVVYSTKPNKDNITYID
                     SDVIRASTFNKIINYDKNGDSHYDILSAFQKSIRGSDPQASIHYLARLIKGGDLISIC
                     RRLLVIASEDIGLAYPNAIVIVKACVDSAMQLGFPEAKIPLAEATILLATSPKSNSAC
                     IAIMKAMDELDKEFVKDIPNSIKDAHYSGSKEIGRGCGYKYPHNFKNHYVKQQYLPDS
                     IKDSIYYIPQENKTEKNIKKYLEYLNSDF"
     gene            complement(21283..22068)
                     /gene="codY"
                     /locus_tag="PC414_001710"
     CDS             complement(21283..22068)
                     /gene="codY"
                     /locus_tag="PC414_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HDF2464107.1"
                     /translation="MASEVLQKTRKINKTLQTSGGSSVSFDLLAGALGDVLSSNVYVV
                     SAKGKVLGLHLNDVQDSSVIEDEYTKQKKFSDEYTQNVLKIDETLENLNGEKILEIFP
                     EEHGRLQKYTTVVPILGSGQRLGTLVLSRYSNSFNDDDLVIAEYSATVVGLEILRAIG
                     EELEEEMRKKAVVQMAIGTLSYSELEAVEHIFAELDGKEGLLVASKIADRVGITRSVI
                     VNALRKFESAGVIESRSLGMKGTHIRILNDKLTDELKKLKNNQ"
     gene            complement(22233..24320)
                     /gene="topA"
                     /locus_tag="PC414_001711"
     CDS             complement(22233..24320)
                     /gene="topA"
                     /locus_tag="PC414_001711"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HDF2464108.1"
                     /translation="MAKTLVIVESPAKAKTIEKFLGKSHYTVKASVGHVRDLPKSKLG
                     VDIENNFEPQYINIRGKGDVIKELKKEAKKSKQVYLATDPDREGEAISWHLAHILNLD
                     ESEDCRIEFNEITKDAIKKAIKHPRNIDINLVDAQQARRVLDRLLGYQISPILWQKVR
                     KGLSAGRVQSVTTKLICDREKEIKAFIPKEYWTIDVEAKTVKDKDITLKFYGKNDEKI
                     ELENEEAVNEILKEMEGKDLKVEKIESKSRRKSAPKPFTTSMLQQEGANKLFFTTKKT
                     MMIAQELYEGIDIEGEGTVGLISYIRTDSKRISDEAKEKAKGYIVNNLGENYYKNIGD
                     KKEKKDTKKVQDAHEAIRPTSVDRTPESIKASLSKDQYKLYNLIWRRFVASQMEDSVF
                     DILNVECKIGDIVFKATGSKMKFDGYTKVYNFSEREDKILPSIEKGDILNIENILPDQ
                     HFTQPPARYTEASLVKTLEELGIGRPSTYAPTIATILNREYVEKQGTSLCPTELGIIV
                     TDILENNFHKFIDVDFTADMESKLDVIEEGNTDWKKIVSESYAPLKEAIEEALENIEK
                     VNMDEETDEICENCGSNMVIKYGRFGKFMACKNYPDCKTTRPIMNKIGVKCPKCEEGD
                     IILRKSKRGKAFYGCSNYPNCDFVAWNKPTGEFCEKCGSYMVEKVTKSETKTVCSNKE
                     CNKETKEHNDGKE"
     gene            complement(24353..25453)
                     /gene="dprA"
                     /locus_tag="PC414_001712"
     CDS             complement(24353..25453)
                     /gene="dprA"
                     /locus_tag="PC414_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDF2464109.1"
                     /translation="MEKRDTYLWLKSIGGITTKTIEIIENEIVNIEDIFDFSEKEIYN
                     LKNISLNIRKNIVKYRGHAYLENIKELLYKKAIKYICKYDKEYPENLKNIYNAPKLLF
                     YKGDIGLVNNNFNIAIVGSRKPTAYGINCAKTISCQLSQYGVNIVSGLAIGIDAYSHI
                     GCMSGKSKTIAVLGSGVDNPLPKQNLHLSNKILENGGLLLSEYNINSTVAPYHFSNRN
                     RIISGLSDGVVVVEAAIKSGALITVDFALEHGKNVFAIPGNINSQMSRGCHKIIKEGA
                     KLIENIDDILNEYNIFNIIDKKINQKYDNISLNAKSKQIIEAIKREGNLHIDSICDYT
                     GIEIKYVNSIINELVLNELVVEMNNKTYSLNV"
     gene            complement(25472..27073)
                     /locus_tag="PC414_001713"
     CDS             complement(25472..27073)
                     /locus_tag="PC414_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HDF2464110.1"
                     /translation="MLSIINSSNLVGIDSFLVKVEVDVSNGIPSFNIVGLPGKEIKEA
                     RERVKSAILNSGYKFPSTRIVVNLSPADIKKEGAFLDLSISIGLLRELIKKDENYIRE
                     SMFIGELSLDGKIRKVRGILPIIMGAKTQNIKRIFIPIENIKESLFVDEIDIIPIKSL
                     KECVDFLNEEIKVDKVRIMSFLDDKSRKENEELEKDNSYIDCKYTKINNEESKYDEDF
                     KDVKGNYFVKRSAEIAAAGNHNMFMIGPPGSGKTMIAKRVRTILPDISVEEMIEVSKV
                     YSILGMINETKGIIDKRPFRAPHHTTTKQSLIGGGMDARPGEIALAHRGILFLDEIAE
                     FDRKILETLRQPIEDGYVNISRVKYSAKYPCRVLLVAAMNPCPCGYYMSETECRCRSN
                     EIDRYINKISGPLLDRFDIFVEVNSIKYSDFNSLKQEESSQKIKRRVENARKIQINRF
                     KKDNIKNNSEIKAYNLFKYCKLEKEASKTAEMIFSKYNLSSRSYTKLLKMARTIADLE
                     ERDLINSQCIIEAFSFRKAYYSYFK"
     gene            complement(27104..27448)
                     /locus_tag="PC414_001714"
     CDS             complement(27104..27448)
                     /locus_tag="PC414_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HDF2464111.1"
                     /translation="MNNKEKGDFGEKVAVNYLLSKGAKILEKNYRLKIGEIDIIAKME
                     DEIIFVEVKSRSNIKFGYPCESVSFKKRKKIIGVANYYIIKNNLNNTPIRFDVIEVYL
                     LEKRINHIMNAF"
     gene            27632..28795
                     /locus_tag="PC414_001715"
     CDS             27632..28795
                     /locus_tag="PC414_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2464112.1"
                     /translation="MNREKLVAFIRYLSLFILVVSYLKNVENTLYPVITMIILFLIII
                     NNQVRFFSLSNENKFILISYFLELILIFILCNYTKTFNSIYFVPLILDISFLIKEKYK
                     YILFSFVIISSLIISLDKNIYLALESSSILLIITVLSIYIENENLSKLYSQNLYDKLR
                     ISKDELKKVNADLETYASSIEELAILKERNRISREIHDSVGHSLSTTIIQLNAIEKLL
                     KDKPLIYDLVHELREFVTESFQDVRRAISELKPVEYENYQSLFKIKELVKSFIKLTNI
                     NVKLTISKNTWNLSRNQSIALYRLIQESLSNSSMHGKATEIRIFITFNTSNLIITISD
                     NGIGCGNIKKGNGLNSINERIYELNGKVEFDNSNNGFTIRASFPKFSGGDFFE"
     gene            28788..29435
                     /locus_tag="PC414_001716"
     CDS             28788..29435
                     /locus_tag="PC414_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2464113.1"
                     /translation="MNKIKVLIVDDEKLIRKGLKIILSSYNDLEIVGDASNGYEALEF
                     CKMNDVDIVLMDIRMKVCDGVLGTRLIKEYNNSITLLILTTFNDDEYIKDAMKFGASG
                     YLLKDSSDEVLHEGIRSSFFGNIVLDKSVAEKIMTSEKTIKQEHLYDMYNLTEKEISI
                     IRLIANGLNNKEISQELFLSEGTIKNNITNILAKLELRDRTQLAIFAFKNKIVIE"
     gene            29564..30499
                     /locus_tag="PC414_001717"
     CDS             29564..30499
                     /locus_tag="PC414_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2464114.1"
                     /translation="MDIVRVNNITKRFNDKLVLDNISFSVKKGEIFGLIGPNGAGKST
                     LINIITNLMLPNSGSIQINGLDLSKDYIKAKSIIGLVPQELAIIETLTPFDNLEYFGA
                     FYGLKGKLLKERIIEALEVTGLTEVKKKKVKKLSGGMQRRLNIGIALLNHPKILILDE
                     PTVGVDPQSRNHIFNFIKDISKKHETTVIYTSHYMEEVEHLCSKIFIMDEGKEIAFGN
                     NDYLKSLVSTNTKLIMEIKNINAQLIFDLKNTKGVISVLENNSLLELDIDKKLQLTDI
                     LSVIDKNNSKIMKISYEEPSLEDVFLNLTGKNLRD"
     gene            30513..31610
                     /locus_tag="PC414_001718"
     CDS             30513..31610
                     /locus_tag="PC414_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2464115.1"
                     /translation="MKLYMSVKTMLKSLKSSFILNLIYFLALPLILSWFLGMVTESMF
                     QNPIKTESTPIVIYDKDNTRLSNDLTKYLKNDLSYILTVKKDDSKAELKLTIPKGYES
                     SLLNEKSNTLNIEKLGTRDDIAILLQDILDTYHEKFYLNNSQKISSEDFSKLFNKNSI
                     DTSIINNNVKQSSYEYFALVSLGFLVIIFIMNNILSNYISESKGLSKRLYSMPITRVQ
                     FLIYDFVGLWIYSFIFLLLYVLFFRILGITFKGNFAILLLLCALSSYFMTSISTFVTS
                     FFSKKYGTAIVYALLFLQTIFGGIFSMISDTFTKLTSLSPTYLIGELFSNYETFKTID
                     SIGNLIFTCLIISTILIALAIVKEKYKWREV"
     gene            31613..32677
                     /locus_tag="PC414_001719"
     CDS             31613..32677
                     /locus_tag="PC414_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2464116.1"
                     /translation="MIRLTIANIKRYLKNHTLLINMVMLPIILIFSLNFFINNSGNQS
                     MYFSPVAIVSDSSGKYEHKLINSSKLKENSFRLNEQDKAMNLLKNNKVSAVFVLDKNF
                     SNSIDKLQRPVVKCFKIENGGGSLWAESQIESFITKSLKLKVDKNIDDKLTKTNIIDN
                     KPDKNKGSFLVVFLICYFMYINAAHLASDLFTLKKSNVLKRLISTDNKDIKIIFSIFL
                     GLFFIQSIVYTFALLCFSIIEDFNLSLNVLMIVLANSFVSTGFVFWVARVFKNESSIS
                     LISTFYSLIGLAISISSLIPSMDKLSFMTNLSKFTPFYWTIDAIKNNGSILINIIALI
                     LIGIIFVTAGSLKLRDFAKN"
     gene            32921..33418
                     /locus_tag="PC414_001720"
     CDS             32921..33418
                     /locus_tag="PC414_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HDF2464117.1"
                     /translation="MYKIGESVMYPKEGACSVNDIVTKKINHEMQKYYELSVIFNSNL
                     KISIPVLNADRIGIRPVMDGNDVDNFIQSINKTDGVWIFDRKERLKLYQDKFHSGDVF
                     EIVKLIKMLMIQDSSKQLCSTDKEFLNKAQKFALSELAAAQCKSYTMVLEEMKKHILN
                     SKNTN"
     gene            complement(33594..33842)
                     /locus_tag="PC414_001721"
     CDS             complement(33594..33842)
                     /locus_tag="PC414_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464118.1"
                     /translation="MSRKAYEEALVELEKFIDERKEIIKSAEDCIDKYIVDRTLPFDY
                     KDKCVEWQQELLDIAEAQVLEANELSVLLQEKKELEED"
     gene            complement(33908..34924)
                     /locus_tag="PC414_001722"
     CDS             complement(33908..34924)
                     /locus_tag="PC414_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDF2464119.1"
                     /translation="MEYNELLKSARENLNGSCKVCKVCNGVACAGEVPGMGGKGSGSS
                     FIENSKSLEKVKVNMRVIHDVSNPDTSIEMFGKKMSAPIFAAPVTGTTLNMGGKINER
                     DYIEPVVAGCVNSGIYAMVGDTAVDAFLMENLDVVKKYNGAGIVFIKPWDNENIIKKI
                     RLAEEAGAFAVGVDIDACGLVTLSLHGKPVLPKNVKQIKELVKSTKLPFILKGIMTVE
                     DALMAVEAGVDAIVVSNHGGRVLDCTPGACEVLPKIADAVKGKVTILADGGVRTGLDV
                     LKMIGLGADAVLIGRPFVTASFGGATDGVETYVNKLQSELSSSMILTGCQTIKDIDGK
                     VIYK"
     gene            complement(34982..35749)
                     /locus_tag="PC414_001723"
     CDS             complement(34982..35749)
                     /locus_tag="PC414_001723"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HDF2464120.1"
                     /translation="MQDKSVREIKEIIETLEVEKYMEYIELLRVDERKSVQGLAIKLA
                     KKLDNIRKEEERLETINIFENEGYDKGYLYIGGIDEAGRGPLAGPVVASVVVFKKDTK
                     IEGVNDSKKLSEAKRDELFEVIKEEALDYGIGIVNNEEIDEFNILNATYMAMKKAINC
                     LKKAPDYLLVDAATIPGIDISQNPIVKGDSKSISIAAASILAKVTRDSIMYQYDRVYP
                     EYGFKSHKGYGTKEHYEAIEKYGITPIHRKSFLKNIL"
     gene            complement(35817..38006)
                     /locus_tag="PC414_001724"
     CDS             complement(35817..38006)
                     /locus_tag="PC414_001724"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HDF2464121.1"
                     /translation="MKYIIKRDGTKELFSKSKIEDAVYKASINSEGGVDRELAYGISR
                     KIANDYNSMEKCLSVEEIQDIVEVLLMESNRKDIAKHYILYRERRSEIRTKAWEFDEL
                     QKSIWKNKYRHNGESFDEWIKRISNNSSRISKLIRQKKFLFAGRILANRGLQKEGKKV
                     TYSNCYVIEPPKDSIEDIFDTAKKLARTFSYGGGVGIDISKLRPKGAKVNNAAKHTTG
                     AVSFMELYSMTTGLIGQRGRRGALMISMDVNHPEIEDFIDIKTDLEKVTKANISVRVT
                     DEFMQAVREKKLYNCTFEVETTGEKIVKIVNAYDLFNKFVKNNWDYAEPGILFWDKIK
                     TYNFMSEDKNFCFSGVNPCAEEPLPSGGSCLLGSINLSEFIIQPFGEHATFDINKLKS
                     CVREAVVALNDVLDEGLNLHPLQEQRDSVHKYRQIGLGVMGIADMLIKMGIRYGSDES
                     LELCDLIGKTMLNEAVKQSALIAKEYGTFSEYKYENISKSKFFIENLNEDTKEMVKKY
                     GLRNSQLLTIPPTGSISTMIGISGGIEPIFNLSYIRKTESLHDEDVYYKVYTPIVKEY
                     MDKKGITDEECLPDFFVTAMNIGYEERINMQKVWQKYIDASISSTINIPFETTVEDVF
                     NIYIKAWEAGLKGVTIFRDGCKRSGILINEKKEENNKEDKKENSKKEYYKNNKKEKDN
                     KDEKAKEIINKNFEGDEKFVCPECGSEGIAHTGGCSICLKCGYSGCN"
BASE COUNT        12215 a         6590 c         4090 g        15223 t
ORIGIN      
        1 catcaaaaaa ttactattta tacttaatta tccatattta ttttaagaga attataattt
       61 tatatttata caaaaaaaca caacaactta gagcatttta aatcttttaa aatttaaaat
      121 tacatactaa attattgtga ttttatattt ttattcattt gttgccactt cttgataatt
      181 ttctataggt ggcaattctt caagactttc tatattcaac aactttaaaa attcatctgt
      241 tgttttataa attattggtt tccctatctt attcaatcta ccagcttctc ttataagttc
      301 attttcaaac aaagtttgta ataccttatc acattttact cctcttatat cctctatttc
      361 tacctttgtt attggttgtt tgtaggcaat tatagtaaga gtttctaatg tagcttggct
      421 caaacttttc tttttctttg gttcaataat cttctttata tattctgcat agtctttatt
      481 tgtacacatt tggtacttat tctctaactt gattatttga atacctctat tttgttctct
      541 gtactcctca ataagtaaat ttaacataat ctctatttct tttgatgaaa gttctttatt
      601 tattatatag tttaattctt ttatacttat tggttcacca tatgcaaaca taacagattc
      661 tataatatac tttatatctt ctcttttcat tttaacactc cgaacttttt cttattagaa
      721 tatcatcaaa aaatatatct tgtactacga ctatttcttt ttcttttata agctctaata
      781 tagataaaaa agtagctatt atttcatcat tttcatatga ttttataata tttgtaaaag
      841 taacttcaat cttctctttt ataatatctc ttatatataa tattttttct tctacagata
      901 ttattttctt ccttactatt ttatctagtt tctcatcatt agcattttct atctgtgatg
      961 ttttaacttt aaatatatat ggaagtatat tttttattgc ttctattgat atatcttcta
     1021 aatccacatt gtcatcaata attatctctt ctttatttct ataaaatctc tcattaacat
     1081 aagttatatt ttcttttata tcttgtgatg ctaccttaaa ctttctatat tcttctaact
     1141 tttccataag ttctattctt gggtcttctt cttcatcttt ttgcttataa agcagatatt
     1201 tagactttat ctctaaaagt tttgatgcca tagttataaa ttcactagtt atctctaaat
     1261 ccattttatc tagcattttc aaataattta gatactgctt agttatatct attatggata
     1321 tatcctttat atctatttta tgctttgtta ttaaatcata aagtaaatct aatggtccct
     1381 catagacttg taattgtata ttatacttca tatcaatccc tctttaatct gtttattata
     1441 ttaaacaaat aaatatacta tttttagcaa cactgcatat attggtgtta atattattct
     1501 atacatacca gtaaataaag caattaataa aattatataa ctcatgcttt caaatttata
     1561 caaatattca ttccatttat atggtacaaa tgtagatata actccccatc catcaagtgg
     1621 aggtatcgga agtaagttaa aagctgcaaa tccaatacta tataaaaaca catattcagc
     1681 tattgtattt atagcataca tatgcacata cttattgata attacacaca gtatagcagt
     1741 caaaacatta caaaaaacgc ctgcaagtga aactatcagg tttcctatct tatagttttt
     1801 aaagttattt ggatttactg gtactggctt tgcccaccct atcttaagga taaacatagc
     1861 caaaagacct attgggtcaa catgtcttac aggatttaat gtcattctac cataagcttt
     1921 agctgtatca tctcccaata agtgagctga ataagcatgg ccaaactcat gtattgatat
     1981 tgccatagct ataccaacta aacttgctac tatttctcct atattcatac ttaacattaa
     2041 tgtacatctc ctaaattaaa tttttaatat tattataaca attaaatagc ttataatcaa
     2101 aattatttta cattataata attttaactt ctaggcataa aattaaaatt tgctttttat
     2161 tttataactt ttattattat atcatattca ttgtgaaaat ataataatta cacctactgt
     2221 aagtgtaata ttatattgac atgaattgac attattgtct cttagtcaat attatacaag
     2281 aagtataaaa aaatatgtgt tacactttgt ataacatgaa aggaggttac ctaagtgaga
     2341 caagaagaac gcacagtatg ctttgatagg gaattaaaaa ttgaagcata tagatttaaa
     2401 ggcataatgc aaaaattccc aaaccatttt catgaacatt atgtaattgg ttttatagaa
     2461 aaaggtcaaa gatatttatc ttgtaaaaat aaagagtata ccacaagtac tggagatttg
     2521 ctacttttta acccttttga tagtcatact tgtgaacaaa tagatgataa agtacttgat
     2581 tacagatgta ttaatattaa gccagaaata atgaaaaaaa ctgtctttga aattacaggt
     2641 aaaaattatt taccaaaatt taatcaacct gttatatttc gcagtgagct agtacctcta
     2701 ctacaagaat tacactatat aatcatggaa gaagaattgg attttaaaaa ggaagagctt
     2761 tttttcttcc taattgaaca attaattgaa gaacatacag agccaaattt gcaatctaac
     2821 ttggaaaata caaatataga aattcaagca gtatgtgatt atctagaaaa taattatgct
     2881 gaacatatag tacttgacga attaagcact atagcaggta tgaataagta ctctctactt
     2941 cgtaatttta cgaaattaaa aggtattaca ccatatagat acctagaaaa tatacgggta
     3001 aacaaagcta aaaagctttt agaaaaaggc gtagaaccta tagatgctgc gatacaaaca
     3061 ggatttgtag accaaagtca ctttacaaac ttttttaaga attttattgg tcttacacca
     3121 aagcagtatc aaaatatatt tataaatgat gattcaaaca actttaaata aggtaaaaag
     3181 gagaaataaa aatggaaact aaaaattcaa aatgtgtcat ggtaatagac gaaaatttac
     3241 caatgggaat tatatccaat acagcagcta taatgggaat tacactagga aaacatgccc
     3301 cagaaactgt tggcccagat gtaattgaca aaacaggtaa tagccactta ggaattattg
     3361 acattcctgt acctatttta aaaggaaaca aagaaataat aaaagattta cgtaaaaaat
     3421 tatatacttt ggaatttaac gatttaactg ttgtagattt ttcagatgta gcacaaagtt
     3481 gcaatcttta tgaagaattt acacaaaaga ttgctagtgt tccagaagat gagttacagt
     3541 attttgggat agcaatttat gggaataaaa agaaagtgaa taaacttact ggtagtatgc
     3601 ctttacttag ataattgtta aacaacccta tatatgatat taaatcaaat gatattttaa
     3661 gatacataaa cttttctata tttctataaa aatcataaga ttataaaata cttctatatg
     3721 gtaaataaaa atacccctat ggcaaataat tacataaggg tattttttat gtgtattata
     3781 atcatatctt tgtgtattat aattatattt catctgataa ttatttatat catattatca
     3841 attattcttt gaagcatttg tagatagctt gccttattta tatccttagt atttatcaat
     3901 tccacttcgc ctattacttt tttatcttta catatcttta tttttcctaa aacagttccc
     3961 tttttaattg gcatcttagg attttttact atttcaattt tcttctcaaa ttctttagaa
     4021 cttccttttt taataagagc atttaaatct tctttagcaa ccaatttaac tttgttttca
     4081 tcagccttat ccaatgtaag tgttgctata ttatcacctt tactacataa cttaacactt
     4141 tcataattag caaatccata atttaaaagg ctagaggcat cattaaatct ttctggagaa
     4201 gtctctgctc ctaaagttgc agcaactaga tgagtattac ctctctttgc agatgctgat
     4261 aaacagtatt ttgcttcttg agtaaaacca gttttaacac cagttgctcc ttgataatgc
     4321 tttataagtt tattagtatt tgcaagacct actgtaactt gtttcttccc tactacaacc
     4381 ttatccatcc atgtagttaa gtatttactt atcatttcgt gttttaatag ttctctagac
     4441 attaaagcta tatcatgagc agatgtatag tggtttgcaa ctggaagtcc atttgtattt
     4501 gcaaaatgag tatcattcat atttaactct tttgctttag cattcattct atcaacaaat
     4561 ccttcaacac ttccagctaa gtgttcagcc atagcaacac aagcatcatt tgcagaagca
     4621 acagctatac cttttaataa tgtatcaacc ttttgagttt ctcctggctc taggaatatt
     4681 tgactaccac ccatactaga tgcagtttca cttacttgaa cttcatcatc aagcttaatc
     4741 ttgcctgaat ccaaagcttc tactataagt agcatagtca ttaccttagt tacactagca
     4801 ggaggaagct ttttatgagc atccttttca tataaaattt gtccactacc aacatccatc
     4861 aaaattgctg attttgaaga tatatttagt tggttggcat cagcattttc cttattattt
     4921 gcaaaagaaa agcttaaagg agtaagcgca attaatatag ccaaagcagt actagctaat
     4981 tttttcatcg atgtcacctc ttaaagttta tttaaactta ttttctcaca tttaactttt
     5041 tttattctaa atgcctagat ataccttgac ctaaattttt tttcttttca agctttttaa
     5101 agtaaccttc taaaacacct ttattagacg cattttctat tatttcaaaa gcatcttcat
     5161 aattcattcc atactctcct gctatttcca tttttgtttg ttttaaagct tttatagcat
     5221 tttgtcttga aacatcatcc ataagaatcc tcctatcagt aaaatttata tataagtagt
     5281 ttttactaat aaaagattgt tataattggt attttatgta atatatattt ttttaaaatg
     5341 agtattaaaa agacctcttg taattcttaa gataatatca attggatttt tgatattact
     5401 ttaaaaatta caagaggtct aattttcaaa tttatagttt ttttatatta tacttccatt
     5461 atgataggaa gtatcattgg atttcgttta gttttttgat ataaatattc ttttaggttt
     5521 tctttaatat tattctttaa ataagcccat tctttaatat ttttctcttc acattcattt
     5581 agaacattct taattatatc ctttgctcca tccattaaat cttctgactc tcttacatat
     5641 acgaatcctc tggaaattat atctggacct gctaagacct taccctcatc cttggaaata
     5701 gtaactacaa ctatcataag accatcttct gataaatgct ttctatccct taaaactata
     5761 ttacctacat cgcctactcc tagaccatca actaatatgt ttcctgtttg tatacggttt
     5821 gctaccttag cagaattttt atctaattct aaaacatctc ctgtttgcat gacaaaaata
     5881 ttttctttat ccataccaag ctgttcagct atttcagcat gtctttttaa cattctatat
     5941 tcaccatgag ctggcataaa aaactttggt ctaactaatg catgtataag ttttaattct
     6001 tcttgacagg catgtccaga aacgtgtata tcagctatat cactgtatac tacttctgct
     6061 cctttttcaa ataagaaatt gattactctt gatattaatt tttcatttcc tggtattgga
     6121 tgagcagata ttataactaa atcgccactc tttatttcta cttttttgtg ctctgaaaaa
     6181 gccattctag caagagctga cataggttca ccttgtgaac cagttgttat tatcactaat
     6241 tcactatctt catacttaca tatatcattt aaatctataa gcatgtcatc agaaatatct
     6301 aggtatccta attcttttgc aactccaact acattgacca ttgagcgacc tgatatagca
     6361 acttttctat taaacttttc agcagtattt attatttgtt gtaatctgtg tatgttggat
     6421 gcaaaggtag caactataat tcttctgcca tttccttttg caaataattc gtcaagacct
     6481 accccaacag tcttttcaga catagtaaat cctggttttt ctgcattagt actatctgcc
     6541 agcattaaaa gaactccttt tttgctcaac tcacagattc tatgcatatc cataacatct
     6601 ccatcaatag gagtcaagtc aatcttaaaa tctcctgtgt ggtatattat accttggtct
     6661 gtgtgtattg caatagagta agcatctggt atactatggt tatttttcaa aaactctact
     6721 tccatattat caagctttat aacttgtcta ggacctataa catttaactt aacattattc
     6781 attttatgtt ctttaagttt tacttgtata agacctatac taagtcttgc cccatataca
     6841 ggtacattta ttttctttaa tatatatgga agagctccta tatggtcttc atgtccatgt
     6901 gtaataaata taccctttat tttatcccta ttttttacta aataagttat atctggtata
     6961 actatatcta ctcctaacat ttcatcctct ggaaaactaa gtccagcatc tataacaatt
     7021 atttcatctt tatactcaac aacagtcata ttttttccaa cttcgttaag tccacccaag
     7081 gccattacct ttatcttgtt ggtgttcttt ttgaacaatt gcatcatctc cttctgtcta
     7141 tttaattttt taacacgttc tacaatcata aataatatca aatcacgcac tcgctaccag
     7201 ttattcttat tgtaccataa ttcgtatcat attttaagct atatttaaaa tacatttaac
     7261 gtattatttt gacaaaccta ttttattttg atatcctcta aatattatat gatttaatat
     7321 cggataattt aggaggattt atgcgtggta aaactcattg tgcaatagga atcttaacag
     7381 ctatccaaac atctttaata tttaaaattc ctatatcttt agttgatatt ttagtttcag
     7441 ctacttttgc tgtactaccc gatttggata aatctaattc tatggtctca aatttcattc
     7501 taaaaaacaa tgtatccaaa tacatatata gaatttttat atatgctgtt aatattatta
     7561 ttttctttat ttcaataaat ataaatgata atttttattt aagcgcaata attacatttg
     7621 ttgcaattat aattattgag gcaaaactaa cccacacatt tctaagaaaa gtttttctat
     7681 cattaatatt tatactatta gctatatgct tatacatcat agaagttgaa atatatttta
     7741 caattttttg tctaatgttg tcaatttttc cctggttaaa gcacagaagt ttttctcata
     7801 gcatatttgc aactataata gtatattttt tactaaaaca aatagaatta atcactaata
     7861 ttaataattt atctttttat ggtacgattg gttatgcaag tcatatgttt ttaggagatt
     7921 tatttacaaa gcaaggaata ccaatttttt atccgctaag cgaaaagaaa atttctttag
     7981 gttttttaac tgttggagga ccatttagta atttcataga aaaatcattc atttttgtac
     8041 ttataggatt aattatattt tcaatattaa aactataagc atcttgaaaa ctgtatattt
     8101 gcattttata gatggtttat aataaaaaat ggaagaatag catagaatat aaaagttcta
     8161 tactattctt ccatcgacat taagcaatag aagtttattc ttctgaatcg cttactccat
     8221 tacactcgtc acatagtcca taaaatttaa catcatgatc aagtatttta aatttgtaca
     8281 atgattgtat ctctttttct atattgtcta aaaggtcttc tttagcttcc attatctttc
     8341 cacaattttt acaaataaga tgatgatgat tatgacagtc ttctttatgt agactaatct
     8401 catatcttat acatccatcg tctaagttta gttttgatat taatcctatt tcatccaata
     8461 gctgcattgt tctatataca gtagctaagc ctatttcagg acagtcaacc ctaactaaat
     8521 catagatttc ctcactactt agatgtgaat ggtcatgttt aagcaatatt tcaactattg
     8581 ctcttctttg tggcgtaatc ttaaaaccag tttcttttaa cttgtctttt aataaatcca
     8641 ttgtatttgc cataacatca cccttactat tttcaattga taatctttat taaataatat
     8701 tgtaattgat aattcatttt ttgtcaagac atttaaataa atttaattta ttctcataat
     8761 ataaatatat acccataaaa gatatattat tcaccaaaat tcataaattt tttggtattt
     8821 ttaataaaaa acaaaaaaac agatatatac tataaatgct tttacaatta ataatgttta
     8881 attaaagcaa atataataaa tatctgattt ttatatacaa acttaattac ttatctagtt
     8941 aagtccattt gattcatctt cgtcgtaatt taaaccttct tcttccatta tagcagtata
     9001 cacagctaca acagtttcat actcttcatc actttccaaa ggtattaaga tttcaccttc
     9061 ttcatcctca tcaattctaa agattaaagc ttcttcagct tcttcatcat ctaaaggcat
     9121 taatattgca tactcttttc cttctgcttc taatgttaat attatttcaa attggctttc
     9181 aacgccattt tcgtctatta aatttattat attttcttcc ataatgtcat tcacctcaaa
     9241 tttattttta tttttattat ttatattata tatcataagt attcaatttt atagttcttt
     9301 tatgaattta ttttaaaatc taaatatcct tgcaaaatta ttactgctgc caacatatct
     9361 ataacctttt ttcttttttg tctactaaca tctgcttcta ataaagacct ctctgccgct
     9421 actgtagaaa gcctctcatc ccaaaactct acatcaatac ctgtttctgc ttttaccaat
     9481 tcacaaaact tgattacttt ttctccttgt ggacctaaag ttccattcat attttttggt
     9541 aatcctgata caattttatt tacttgtttt tcttttatta ttgcttttag ctcttcaata
     9601 tcttttttct tgccaactct ttttattgtt tttacacctt gtgcagttaa tcccatcaaa
     9661 tcactcacag caactccaat agttttatca ccaacatcaa gacccattat tcttccatct
     9721 aacatagttc ctcctttatc tatttatatt ctatagatta aatacatatt ctgaacctct
     9781 cataactaag gtcacaggct tactgcttca tataatttta cataatcaat cataaaaatt
     9841 gcaatatgat tttttatgta agcatccagt ttacattaat aatcatattg cattttttat
     9901 ttaaatccta tatgaacagt aaaaattttt ttagttattt ttttcaaata ttatcatatt
     9961 tatacttatt attattagtg gtataagtat atatatagat gctgtagatg cagcaactat
    10021 tccagaatca ttaactaaaa gtgtaattat acaaccaacc atagatgcaa taaacccttt
    10081 aaacaagatt ggaaaattat tcattatctt tctaaagtgt ctagatggtt taaatataag
    10141 cactgcaatt actccaattc ctacaagtaa aatatttacc catacacttg tctgtgctag
    10201 ttgcatattc atttgaattt ttcttccaaa tgtttgtaat atctctcctg gtccatttag
    10261 gaaaattctc tgtacaaaca tacctaaatg agattcacta cctgaaagta tatctaaagc
    10321 tgcaaatgca aaaacgacaa ctacagctga aacagctaat aatacagctt tcttaaaatc
    10381 tattttaatg tcatatataa gcattataaa caacaaatat gctacacatt ctgatatagc
    10441 tccaccaaca tttgcaccca ttgaaggata tgcactagtt ataagtatta ttaatgaaaa
    10501 aattacaaca agtaatttag ataccttctt ataatgtaat aatactgcta agccaaatac
    10561 tgcacttgct atagttacac cttcatattc atttcctaca ccatagtacc tagctcctat
    10621 gacagcatca taactcatta tgttattttg cattaagtat gttccaaata cagaatctat
    10681 aactatgact aaaatagtaa gtgttgcaaa aaatcccatg tgctttatat cattgtcctt
    10741 aaataatttt ctacctaaca agtataatat tagcgtagtt gcaaatattc ccaaagatat
    10801 tgaaattgga gttttaaaat tcatcaaagg agctaacata aatgatagtg gcattattat
    10861 gcctaatttt ataaactcct taagtacttt gaatacacgc tctttatgtg gcatataatt
    10921 tctaaagaaa atcgcaaaca tggcaattac ccaagaagca gatattacgc ctacaaagcc
    10981 atttataata gttgacctta ttgaagatat agacactatc ctttcataaa gtgattttag
    11041 atagctaata ttgtcctctt tatttactaa agtataactt ctacctacca tctctttatt
    11101 ttcaagacca aatgtaccta gtatatctac tccaacatct aaattggcaa caattccagc
    11161 ctttcttgtt gtggcagatt ccagtagacc tttcccttct ccactaaatt ttattattgg
    11221 agaaagtctt ctcttattct tataatctag gtcacttgga aatgcacttg ctatataaac
    11281 agtgtcattt tctccaacca tagaaaatac ttgttcaaga tatttattta tattttttac
    11341 aatatttttc ttcattatat cataagtatt atcatttaga tttaatctat acatatctaa
    11401 tctatatgta tctccaagtt ctacaaatat agcatcacta tttttatagg attttttagt
    11461 ttcatctagt aatttatcat aatctgtaga tattccaaat ggcattttag aatctttctt
    11521 attaatatta tcaatattac ctgaatcaat ccttccatat tggtccatag ctactaaaca
    11581 taagtttctg tttttaacta attgattatt ttcaattata tcagcatttc caattacaga
    11641 tgtctttaaa ccattttctg ataaagtttt tcctaatgac cctaaatttg ctccatattc
    11701 tccatactca agattttcat ttatagatct attaatgcct aaatcattta tttctttagc
    11761 tttttgacct gttgcgactt caaaagcttt agcagtatct tggtctactt tttgaaaatt
    11821 gatatagtct tcagtagtta cactagctct tgttcctgca cccatacttg cataagacct
    11881 tctatcatca gtaccttgat cccctcttat attcatgaga gctacatagc ctctcttttc
    11941 tatctcattt ttcaagattg gaatattcat catacttcca agattggtcc tattcatatc
    12001 aataaatatg accttgcctt tttcttcagc aaaaccatag ctagcagttg atacaaaaat
    12061 cataatgact attgcaaaca acgacataat cttttttctc atttatttga cctactttct
    12121 attcagatag tgcgtgatta cttcttcaag tatctcatct ctttcaaatt ttttaattat
    12181 agctcttgca tttttatgac tagttatata gctagaatca ccagaaagga gataccctat
    12241 aatttgattt atagggttat atcctttttc aactaatgct ttatatacaa aatcaatagt
    12301 atctcctaca ctcatcctat cttctggtat tccttcaaat ttcatagtgt agtctaaatc
    12361 tttttccaaa ctaaaaacct ccttgagtga atgttattta acttgtgtct taataacctc
    12421 acttgcatag cttaaagctt ggtcaacctt agatacatca ctagctcctg cttgtgccat
    12481 attaggtctt cctccacctt ttcctccagc tatttttgca acttctctaa caatattacc
    12541 tgaatgtatt cccttatcta atacatcttt agtggcagta actacaaagt taactttatc
    12601 atctactaca tttgcaagta ctactacacc agaaccaagc ttatctctta gattatctgc
    12661 tgtttctctt agtgtatcca tatccatacc ttcaaattta ttagttatca aattaactcc
    12721 atttacttca actttagaat caaaaataga atcaactgat tgtaaactca tcttagcttt
    12781 catatcatgc aattctttag ataagttttt attttcttct aataaagaac ttattctttg
    12841 aattaaatta tcttctttag attttaagtt tacacaaact tcagagataa ttccatctct
    12901 ttcttttagg tattcataaa cagcttttcc tgttattgct tctattcttc ttacacctgc
    12961 tgctactcca ccttcagata atattttgaa cattcctact tgagaagtat ttgtcaagtg
    13021 agttcctcca caaagttctg tagaataatc tcccatagaa actactctta cctcatcacc
    13081 atatttttca ccaaatagtg ctgtagctcc tttttcttta gcttccttta tattcataat
    13141 atcacatttt atgtctaaag atgataaaat aacattattt actttttctt ctattacttt
    13201 taattcttca tttgatatcg cttcaaagtg agtaatatca aatctaagtc tttctggagt
    13261 tactaaagaa ccagcttgat taacatgttc tcctaatact tctcttaatg ccttatgaag
    13321 taaatgtgta gcactgtgat ttcttgcagc tgacattcta gtttctctat ctacgagtgt
    13381 cttcactttg tccccattac ttattcttcc tgattttatt atacctatat gttttatact
    13441 attattagca ccttttttag tatttaatac ttctaaaact aagttttcat tagttatcaa
    13501 tccacaatct ccaacttgtc ctccaccttc aggatagaaa gtagtgttat ctagtactat
    13561 agatacctta tcgccttctt ctgcactttg aactagctca tcatctttaa ctatagcttc
    13621 aatagttcct tcaccataaa tttcactata cccattaaat gtactgtcaa ctgtagattc
    13681 taattttgat agagggtctt ccttccaact ttctccatcc atatttcctc tagcatttct
    13741 agctctttct ttttgctttt ccatttcttc attaaaagct tcttcatcta cagatagatg
    13801 ttcttcttct aatatttctt tagttaagtc tatagggaag ccataagtat catatagttt
    13861 aaaagcttct tgaccactta ggacagtctt tccttcattt tttaatactt ctatataaga
    13921 gttcaatatt tctgttcctt ggtctatagt ttcattgaat ttttcttctt ctattccaac
    13981 tactttttta atataacttt ctttttcaac taaaactgga tatgctttat catttacttt
    14041 tataacttca tctattagtt tctgtaaaaa taattctttt actcctaata acttaccatg
    14101 acgagcagca cgtctaagga gtcttctaag aacatatcct cttccttcat ttgatggaag
    14161 cactccatca ctaactaaga atgttacagc tcttatatgg tcagttatta ttcttattga
    14221 tatatcattt tttggattct ctccatattt tactcctgtt aacttctcaa ctgcttctag
    14281 tatagattta attgtatcaa cttcaaagat agtgtctacg ccttgcataa tacatcccat
    14341 tctttcaaga cccatacctg tatctatatt cttgttttct aatagactat aatttccttc
    14401 ttcatctcta tcaaattgag taaatacatg gttccaaaat tctaaatacc tatcacaatc
    14461 acagcctggt ttacagtctg gattgtcaca tccatattct tctcctctat caaaatatat
    14521 ttctgaacaa ggaccacaag gacctgtacc tatttcccag aaattatcat cttttccaag
    14581 tctaaccatt ctttcttctg ggaaattcat ttcttttgcc catatatcat aagaatcatc
    14641 atcttcttca tatacagtca cccatatctt atcttctggt atatttagcc attctgtaac
    14701 aaattcccat ccccatttta ttgactctct tttaaagtaa tctccaaatg agaaattacc
    14761 catcatttca aagaatgtag catgtcttgc agttatacct acattttcta tatctcctgt
    14821 tcttatacat ttttgagatg tacacattct cacacttgga ggtacttcta cacctgaaaa
    14881 gtagtttttc aaaggtgcca tacctgagtt tataagtaac aaactcttat cattgtttgg
    14941 tactaaagaa taactatttg ctacataatg accttttgac tcaaaaaatt ctaaaaactt
    15001 acttcttatt tcatttaatc ccatcttttc catatcatgt tcctcctaat aaattttatt
    15061 ttttcgttta aataaaaaac ctcgtcccta taaataaatt tataggggcg aggacaatcg
    15121 cggtaccacc ctaattatca tacaaagtat gatatcttcg gaaaacctta gctataacgt
    15181 aactaccgtc aatctctact taaatcttct ttataaaaga taaattgttc aagattgcta
    15241 ctaaaaggct gcttcaaata tcatattcta agggcttcca gcatttccct ctctctgtaa
    15301 gacatcaaat attttactat tccttatcac tgtatttgtt ttttaattaa tatactaatt
    15361 atattactaa attttacaaa attaatcaat actatatttt catatacaat aaatttattt
    15421 tataaacttt atttactctt aatatacttt attgcttatt tttttaattt tttagatgtt
    15481 tttataaact tttctcgatt atacctccac ctactacaat atctccatca tacattacta
    15541 cagattgacc tttagtaata gctctttgag cttcatcaaa aactatttta atagtatctt
    15601 ctcctactct atgaatagta gctggtgatg gttttgctga atatcttatt ttggcttgaa
    15661 ctcttaaggg ttcttctaac gtatctattg atataaagtt aacatcttta gcaataagtt
    15721 cctttttaaa taaatcttca ttgtctccaa gaactatggt attatttatt ggatttatgt
    15781 ctattacaaa cattggtttt ccaaaagtaa ttccaagccc ttttctttgg cctatagtat
    15841 aatataatat acccttatgt tttccaagta tattgccttt agtatctaca aaaaaacctt
    15901 cttcaatacg ctttttagaa tgttttttta cataatttgc atagtcatta tctttaacaa
    15961 aacaaatttc ttgactatca ggcttattgt gtactgccat acccatttcc ttagcaattt
    16021 ctcttactct atctttttca tagtcaccta ttggcaataa agtatgctct agttgctctt
    16081 gtgtaagatt ataaagtgca taagtttggt cttttttatc tgtaacagat ttttttagca
    16141 aatatcttcc tgtactttca tccttttcta tctttgcata gtgacctgta gctacataat
    16201 cacaacctat ttgtctagcc ttcttataaa aatcatcaaa ttttatatgt ttattacaag
    16261 ctatacaggg attaggagtt tttccttcca aatattcatc tataaaataa tctattactt
    16321 tttctttaaa cacttctcta aaatttaaaa cataaaatgg tatacctatt ttatttgcta
    16381 cccttctagc atcttcaaca gcagataaag agcaacaacc accttcaatt tcaacaacat
    16441 catcatcttg ccacaacttc atagtaacac caatgacatc atatccttgt tgttttaaaa
    16501 gatatgcagc tacagagcta tcaactcctc cactcatacc tatcattact tttttattca
    16561 taaacaaatt tcacctcatc ttttgcatta cttattatta taccatatag agcaagtatt
    16621 gtacttattt aaaatatagc ttgttctatt ttctaaaaaa ataatcatct ttattatttg
    16681 aatcttatgg attattttgt taactaatta acctctaaat caagttaaaa tacttaattt
    16741 aggcaataaa aaatagctat tttaaaaaca gctatttttt atgtcctaat tatatcatta
    16801 gtacttcttt ttaatcattg taaacttctc ataacacaat cacttatttt tattcttctt
    16861 cttcaacatc atggtcatga gcatcatcta taacatatcc atccaattct ggtatatgga
    16921 tattgttctt ttgagcataa tctataagag ctgcttttac agcttgttca gctaaaactg
    16981 aacaatgcat ctttactggt ggtaaaccat ccaaagcttc tgcaacagct ttatttgtaa
    17041 gttctaatgc atcttttata gtttttcctt ttatcatttc agttgccata gaagaacttg
    17101 ctatagctga accacagcca aatgttttaa acttaacatc ctttattaca tctccatcta
    17161 tgtctaaaaa tatcttcatt atatctccac atgtagggtt tccaacttca cctacaccac
    17221 ttgcattatc aatttctccc atatttcttg gattcataaa atgttccata actttatcac
    17281 tatattgcat tatcttttcc tcctactcaa tttatgtcta ttttttatgc tttatcttta
    17341 tgtctatttt actaaacctt gtttcttcgc atcatcataa agtggtgaca tgcttctaag
    17401 tctttctatt acttttggta agttctctag tatataatct atatcatcat ctgttgtaaa
    17461 atctccaact gtaagtctta atgaaccatg tgctatttca tgtggtaaac ctattgcaag
    17521 aagtacatgt gaagggtcta atgaaccaga agtacatgca gaaccacttg aaccagcaat
    17581 tcctaacata tctaataata aaagtattcc ttcaccttct ataaattgga atgcaaagtt
    17641 tgcatttcct ggcaatctat tttctaagct accatttact cttgtgtaag gtattctttc
    17701 taacacacca tctattaact tatttctaag acgagttaaa gtttctacat gattttgcat
    17761 attttcctta gctaattctg ctgcttttcc atatccaact attgcaggta tattttctgt
    17821 accagctctt ttgctttttt cttgtgctcc accatggaca aagttatgaa gtcttacacc
    17881 ttttcttatg tataaagctc ctacaccttt tggtccatat attttatgtg aagacatact
    17941 catcaaatct attcctaatt ctttaacatc tactggaata ttacctgtag cttgtactgc
    18001 atcagtgtgg aataatatct tatgtttttt agctatttcg gatatttctt taataggctg
    18061 tatagttcct atttcattat ttgcaaacat tatacttata agtatagtag tatcttttat
    18121 tgaatcttct agttgcttta agtctacttt tccttcgcta tcaacatcta aataagttat
    18181 ttcaaatcca tgatgttttg ctaagtattc acatgtatga agtattgcat ggtgctctat
    18241 ttttgaagtt attatatgat tacctttgtt tttattagca taagctacac cctctagtgt
    18301 ccagttatca ctttctgaac cacctgctgt aaaatatatt tcactaggtt cagcatttat
    18361 aagggctgct acttgttctc ttgctttatc caaagcatct tttgcttctc ttccgaatgt
    18421 gtgaaaacta gatgcattac caaaataatc tgttaggtat ggcatcattg catccaatac
    18481 ttctttttta ataggtgttg ttgcagaata atccatatac aatcttcttt tttccattac
    18541 attcatctcc aaatctattt tttaatattt gttatatcat tttctaattt atttttgtta
    18601 tagtcattta tcatatcttt aagagtaatt gaatctataa catcttctat accctttttc
    18661 atcttttccc aaactatttt tgttacacac atgtttgagt tttcacatac atcttcatct
    18721 agtacacagt cagataatgc aactggtcct tctaatacta caagtatatc tcctactgtt
    18781 atgtcttcag cattctttga aagcaaataa cctccttgag cacctctcac actttttact
    18841 agacccgatt tttttaaaga tgaaaatatt tgttctaagt actgatctga tattttttgc
    18901 ttcttagcaa taaattttaa tgatactggt ccattgtctt gatttaatgc taattcaaac
    18961 attgctttta gtccatatct acctttagta gacaacttca tatattatca cctctatata
    19021 gaaatctaat tcatactgtt ttagtaggaa ttactttcaa gataatttta ttatacccta
    19081 ctaattttgt caagattaat tttaattttt tattttgaat aaattcccca ttttccaatg
    19141 ttttcaaatc ctagtttttt atatatcttt ccagcctctt cattatcata aaacaaacaa
    19201 ggaattttgt tttctctcag taactctgaa catagtttaa ttaggcattt tgtagcaagt
    19261 cctttagctc tatatttagg atgtgttcct actccaatta tcattgcatg agtcctattt
    19321 tctgaagtac tctttgccat tgacaccacc tgtttgttaa tctctataca atatcctcta
    19381 cctgtcctta gtccattttt taaattctct aaagttgtac tgtgaaattc atctattaag
    19441 tcataaagtt ttactgtctt ttttagattt ccaagcctaa ttctcttaac ctttgcatta
    19501 caattattat ctatcaaaaa ctttttagtt tgaagtttgc aaaaatcaac tacctttaaa
    19561 ttattaagtc ctaatctctt tgctaaagca tctacagcac atattttccc agatatttct
    19621 tcgtaattag tattacgaag tagattataa aaaccttcaa catcatactc tccatcagaa
    19681 taaaacatca taaattcaaa atatttaagt aatattgcct ttatttcacc atgttcaccc
    19741 acatctgccc atatatttaa aaaattattt ccatatccat atctttcaat atcccctatt
    19801 ataaacaaat tgtactcagg ttcttttttt aagtatttca taactctact gtgataagtt
    19861 gagttaagtt ttataatcat aatatctacc cccgatacat aaagttcagt ctaaaaatca
    19921 ctatttagat attctaaata tttttttata tttttctcag ttttattttc ttgtggtatg
    19981 taataaatac tatcttttat gctatctggt aaatactgtt gctttacata atgattttta
    20041 aaattatgag gatatttgta tccacaaccc ctacctattt ctttagaacc agaatagtga
    20101 gcatccttta tactatttgg tatatcctta acaaactctt tatccagttc atccatagct
    20161 ttcattattg ctatacatgc agaattagac tttggagatg tagccaaaag aatagtagct
    20221 tctgctaatg gtattttcgc ttctggaaat ccaagttgca tagctgagtc tacacatgcc
    20281 tttacaatta caatagcatt tggataagct aaacctatat cttcactagc tattactaaa
    20341 agtctcctac atatacttat caaatcccca ccttttataa gtcttgctag atagtgtata
    20401 gatgcctgtg ggtcacttcc tcttatagat ttttgaaatg cacttaaaat atcataatga
    20461 ctatcaccat ttttgtcata gtttataatt ttattaaatg tagatgccct tattacatct
    20521 gaatctatat aagttatatt atccttattt ggtttagttg aatagactac cacttccaaa
    20581 atatttaagg ctcttctcat atctccatca gaaagaattg ctatatcttc aatagcatca
    20641 ttattacact caatatccat atagctatct tcatttaata cttcaacagc tcttttcagt
    20701 cctttttcta tatcaagttt ttctaaaggt ttaaattcaa atacagtaga cctacttaaa
    20761 agagctttat atacataatg atatggattt tctgtagtgc tagcaatcaa agttatactc
    20821 ccattttcca taaattctaa tattgattgt tgctgttttt tattaaaact ttgtatttca
    20881 tctatatata ataggactcc atttacatta tttatacttc caagctctga tatcaccttt
    20941 tttatatctt ctaaagatga attagtagca tttattttat agaagctttt attgcttctt
    21001 tctgctatta tttccgctac tgtagtttta ccaacaccag gaggaccaaa aaatatcata
    21061 tttggtaaaa aattagattg taatatctta gacaatattt ttccattacc tattatatga
    21121 ctttgaccta ttacatcatt cattgttttt ggtcttattt tatctgctaa aggcattttc
    21181 tcactccttt ttaatatact tatcattacc ttaataattc gtctaaaaaa ggtatctatt
    21241 tgaaatagat accttttcta atctaaacct ataaaatata gattattgat tgttttttaa
    21301 tttttttaat tcatctgtaa gtttgtcatt aagtattctt atatgagtac ctttcatacc
    21361 taatgatctt gattctatca cacctgcact ctcaaatttt ctaagtgcat ttactattac
    21421 agacctagtt ataccaactc tatcagctat cttacttgct acaagtaaac cttcttttcc
    21481 atccaattca gcaaaaatat gttcaactgc ttcaagctcg gagtaggaca gagtgcctat
    21541 tgccatttga actacagctt tctttctcat ttcttcttcc aattcctcac ctattgctct
    21601 taatatttca agaccaacaa cagttgcact gtattcagct attactaaat catcatcatt
    21661 gaatgaattt gaatatcttg aaagtaccaa tgttcctaat ctttgaccgc ttcctaatat
    21721 tggaactact gtagtatatt tttgtaatct tccatgttct tcaggaaaga tttctaatat
    21781 cttctcacca tttaaatttt ctaatgtttc atcaattttt aacacatttt gagtatattc
    21841 atctgaaaat ttcttttgct tagtatactc atcttctata actgaactgt cttgaacatc
    21901 atttaaatga agacctagta ctttaccttt tgcacttact acataaacat tagaacttaa
    21961 aacgtcgccc aatgctccgg ccagtaaatc aaaagagaca ctgcttccac cacttgtttg
    22021 taatgtttta tttattttcc ttgttttttg taacacttca cttgccattt aatcatctcc
    22081 tcatgaaatt taggcctttt aataaccttt tatattttct aatattattt tcacatttaa
    22141 tgaaaattta tttttctatt agttgtttac acttgaaaga ttatcataga ttggaacctt
    22201 tttcaagaga attatgtgat ttttttcaat tttcactctt ttccgtcatt atgctctttt
    22261 gtctctttat tacattcttt attagaacac actgtttttg tttcagattt agtcactttt
    22321 tcaaccatat atgaaccaca cttctcacaa aactctcctg ttggcttatt ccaagctaca
    22381 aagtcacagt ttggataatt agaacaccca taaaaagctt tacctctttt agattttcta
    22441 agtataatat ctccttcttc acattttgga catttaacac caattttatt cataattggt
    22501 ctagttgttt tacaatcggg atagttttta caagccataa actttccaaa tctaccatat
    22561 ttgataacca tattagaacc acagttctcg caaatctcat cagtttcttc atccatattt
    22621 actttttcta tattttcaag agcttcttca atagcctctt taagtggtgc atatgattct
    22681 gatacaatct ttttccaatc tgtattgcct tcttctataa catcaagttt actttccata
    22741 tcagcagtaa aatctacatc tataaattta tggaaattat tttccaaaat atcagtaact
    22801 attattccaa gttctgttgg acatagactt gttccttgct tttcaacata ctctctattc
    22861 agtatagtag ctattgttgg agcatacgta cttggtctac ctatacctag ttcttctagt
    22921 gtttttacta aactagcttc tgtatatctt gctggtggtt gtgtaaaatg ttggtctggc
    22981 aatatatttt ctatatttaa tatatctcct ttttctatac ttggtaaaat tttgtcttct
    23041 ctttctgaaa aattatatac cttggtataa ccatcaaatt tcatttttga accagttgcc
    23101 ttaaatacaa tatcgcctat tttacattct acatttagta tatcaaacac tgaatcttcc
    23161 atttgactag ctacaaatct tctccaaatt aaattgtata acttgtactg gtctttgctc
    23221 aatgaggctt ttatactctc aggagttcta tcaactgagg ttggtcttat tgcctcatgg
    23281 gcatcttgaa cctttttagt atcctttttt tcttttttat caccaatgtt tttatagtag
    23341 ttttctccta aattgtttac gatataaccc ttagcttttt cttttgcttc atcagatatt
    23401 cttttagagt cagttcttat atatgatata agaccaacag taccttcacc ttctatgtcg
    23461 ataccttcat ataattcttg tgctatcatc attgtttttt tagtagtaaa aaataactta
    23521 tttgctcctt cttgttgaag catacttgtt gtaaatggtt ttggagctga tttacgtctt
    23581 gatttagatt ctatcttctc aacctttaaa tctttacctt ccatttcttt tagtatttca
    23641 tttacagctt cttcattttc taattcaatc ttttcatcat ttttaccata aaatttcaaa
    23701 gttatatctt tgtcttttac tgtcttagcc tcaacatcta ttgtccagta ttcttttgga
    23761 ataaatgcct ttatttcttt ttctctatca caaattaact ttgttgtaac agattgcact
    23821 ctacctgcac taagaccttt tctaaccttt tgccataata taggacttat ttgataacct
    23881 aaaagtctgt ctagcactct tcttgcctgt tgtgcatcaa ccaaatttat atcaatattt
    23941 ctaggatgtt taattgcttt tttaattgca tccttagtta tctcattaaa ctctattcta
    24001 caatcctcag attcatctaa atttagtata tgagctaaat gccatgatat agcttcacct
    24061 tctctatcag ggtcagttgc tagatatact tgtttagatt ttttagcttc tttttttaat
    24121 tcttttatta catcgccttt tcctcttata tttatatatt gaggttcaaa attattttct
    24181 atatctacac ctaatttact tttaggtaaa tctctgacat gaccaactga tgctttaact
    24241 gtgtaatgac ttttccctaa aaatttttct atagttttag cttttgcagg cgattctacg
    24301 atgactaatg ttttggccat aaaaccatac acccccacta cctaatctta atttatacat
    24361 ttaaactgta cgttttatta ttcatttcta ctactaactc atttagtact aattcattga
    24421 ttattgaatt tacatacttt atttctatac cagtataatc acaaatacta tctatatgca
    24481 agtttccttc tctttttata gcttcaatta tttgcttaga cttggcattt agacttatat
    24541 tatcatattt ttgatttatt tttttatcaa taatattaaa tatgttatat tcatttaaaa
    24601 tatcatctat attttcaatt aattttgcac cttcttttat aattttatga caacctctac
    24661 tcatttgaga atttatatta ccaggtattg caaaaacatt ttttccatgt tcaagagcaa
    24721 aatctactgt gattagtgca ccacttttta ttgctgcttc tacaacaact actccatcac
    24781 ttaaaccact gataattcta tttctattag aaaaatggta cggggctact gtcgagttaa
    24841 tattatactc tgataataat aatcccccat tttctaatat tttatttgat aaatgtaaat
    24901 tttgttttgg aagaggattg tctactcctg aaccaagtac tgctatagtt ttagattttc
    24961 cactcataca acctatgtgc gagtatgcat ctattcctat agctaatcca cttactatat
    25021 taactccata ttgggatagt tgacaactta ttgtttttgc acaattaatt ccataggctg
    25081 taggttttct tgaacctact atagctatat taaaattatt attaactaag cctatgtcac
    25141 ctttgtagaa taataacttt ggagcgttgt atatattctt taaattttca ggatattctt
    25201 tatcatattt acaaatatat ttaatcgctt ttttgtaaag tagttctttt atattttcta
    25261 aataagcgtg acctctatat tttactatat tttttctaat attcaagctt atatttttta
    25321 aattatatat ttctttttca gaaaaatcaa atatatcttc aatatttact atttcatttt
    25381 ctattatttc aattgttttt gttgttattc caccaattga ttttaaccat aaataagtat
    25441 ctcttttttc cataaaatcc acctcaaaaa actatttaaa ataactataa taagcctttc
    25501 taaatgaaaa agcttctata atacactgtg aatttatcaa atctctttct tctaaatctg
    25561 ctattgttct tgccattttt aaaagttttg tataacttct actgcttagg ttgtatttac
    25621 taaatatcat ctcagccgtt tttgaagctt ctttttccag tttacaatat ttaaataaat
    25681 tgtaagcttt aatttcacta ttatttttta tattatcttt cttaaatcta tttatctgta
    25741 tttttctagc attttctact cttctcttta ttttttgaga gctttcttct tgttttaaag
    25801 aattaaaatc tgaatatttt atagaattaa cttctacaaa aatatcaaac ctatctaaca
    25861 atggacctga tattttattt atgtatctat caatttcgtt acttctacat ctacactctg
    25921 tctctgacat ataataacca catggacatg gattcatagc tgcaactaac agtactctac
    25981 aaggatattt tgcactgtac tttactctgg atatatttac atatccatct tctataggtt
    26041 gtcttaaagt ctcaagtatt tttctatcaa attcggctat ctcatctaaa aataagatac
    26101 ctctatgtgc taaagcaatt tctccaggtc ttgcatccat tcctcctcca attaaagatt
    26161 gctttgtagt tgtatgatga ggtgctctaa aaggtctttt atcaattatt cctttagttt
    26221 catttatcat acccaatata ctatagacct tacttacttc tatcatttct tctacactta
    26281 tatctggtaa aattgtcctt actctttttg caatcatagt ttttcctgag cctggaggtc
    26341 caatcataaa catattatga tttccagcag cagcaatttc tgcactcctc tttacaaaat
    26401 aatttccttt tacatcttta aagtcttcat catatttact ttcttcatta ttaatcttag
    26461 tatatttgca atctatataa ctattatctt tttctaattc ttcgttctct tttcttgact
    26521 tatcatccaa aaaagacatt atccttactt tatcgacttt tatctcttcg tttaaaaaat
    26581 cgacacattc ttttaaactt ttaataggaa ttatatcaat ttcatctaca aatagacttt
    26641 cttttatgtt ctcaattggt ataaaaatac gttttatgtt ctgagttttt gcacccataa
    26701 ttatgggcaa tatacctctt acttttctta ttttcccatc taatgataat tctcctataa
    26761 acatactttc tcttatgtaa ttttcatctt tttttattaa ctctcttaaa aggcctatac
    26821 ttatagataa atctaaaaaa gctccttctt tttttatatc tgctggtgat aaatttacaa
    26881 ctattctcgt gcttggaaat ttatatccac tattaagaat tgctgacttt actctttctc
    26941 ttgcttcttt aatttctttg cctggaagac caacaatatt aaaactaggt attccattgc
    27001 ttacatctac ttcaacctta actaaaaagc tgtcgattcc tactaagttg cttgaattaa
    27061 taatacttaa catatttaat cctttccaaa aacattatct tttttaaaaa gcattcatta
    27121 tgtgatttat tcttttttct aataaatata cctctataac atcaaatctt atgggagtgt
    27181 tatttaagtt attttttata atataataat ttgcaactcc aattatcttt tttctttttt
    27241 tgaagcttac agattcacaa gggtagccaa actttatgtt agaccttgat ttcacctcca
    27301 caaatataat ttcatcttcc atttttgcta ttatatctat ttcccctatc tttaatctgt
    27361 aatttttttc tagtatcttt gctccttttg atagcaaata attaactgct actttttctc
    27421 caaaatcacc tttttcttta ttattcatta ttcactccaa tacattaaat tttcactttt
    27481 aaattatggt cagtatacaa ttttactatt caagtttttg gattgatttt atttccatca
    27541 atattaataa atacttctat tgactttatt cttttatgat attattctag ttttcatagg
    27601 aaaagtttat ttttttggag gagtgccttt aatgaacaga gaaaaattag ttgcttttat
    27661 acgatattta tctttattta tattagttgt aagctatttg aaaaatgttg aaaatacatt
    27721 gtatccggtc ataactatga taatattatt tctaattata ataaataatc aagttagatt
    27781 tttttcttta tcaaatgaga ataaatttat acttatttct tactttttag aacttatatt
    27841 aatttttata ctttgtaatt atacaaaaac ttttaattcg atttactttg ttcctttaat
    27901 attagatata tcatttttaa taaaggaaaa atataagtac atattgttct cttttgttat
    27961 aatatcttct cttattatta gtttagataa aaatatatat ttggcactgg agtcaagctc
    28021 tatattgcta attataactg tactttctat atatatagaa aatgagaatt tatcaaaatt
    28081 atacagtcaa aatctttatg ataagcttag aatttctaaa gatgaattaa aaaaagttaa
    28141 tgctgatttg gaaacttatg caagttctat agaagaatta gcaattttaa aagaaagaaa
    28201 tcgaatatca agagaaatac atgatagtgt aggacattct ctttctacaa caataataca
    28261 attaaatgct atagaaaaac tcttaaaaga caaacctcta atttatgact tagtacatga
    28321 acttagagaa tttgtaactg aaagttttca agatgtaaga agggctatat ctgagttaaa
    28381 acctgttgaa tatgaaaatt accaaagttt atttaaaatc aaagaattag ttaaaagctt
    28441 tattaagctt acaaatatta atgttaaatt aactatttca aaaaatactt ggaacttatc
    28501 cagaaatcaa tctatagctt tatatagact tatacaggaa tccctttcca actcatcgat
    28561 gcatggaaag gcaactgaaa tcagaatttt tattacattc aatacttcaa atcttataat
    28621 tactattagt gataatggta taggatgtgg caatataaaa aaaggaaatg gacttaatag
    28681 tatcaatgag agaatatatg aattaaatgg taaagttgaa tttgataatt ctaataatgg
    28741 tttcacaatt agagcttctt ttcctaaatt ttcagggggt gatttttttg aataaaatta
    28801 aagttcttat agtagatgac gaaaaattaa ttagaaaagg tcttaaaatt atattatcat
    28861 cttataatga tttagaaatt gtaggagatg cttctaatgg atatgaagct ttagaatttt
    28921 gtaaaatgaa tgatgtagac atagttctta tggatatacg aatgaaagta tgtgatgggg
    28981 ttctaggtac aaggcttata aaggaatata ataattctat aacattatta attttaacaa
    29041 cttttaatga tgatgaatat ataaaagatg ctatgaagtt tggtgcttca ggataccttt
    29101 taaaagatag ctctgatgaa gtattgcatg aagggattcg ttcttcattt tttggcaata
    29161 ttgtgcttga taaaagtgta gctgaaaaaa tcatgacctc tgaaaaaacg attaaacaag
    29221 agcatcttta tgacatgtac aatttaacag aaaaagaaat atctataatt agactaattg
    29281 caaatggact taataataaa gaaataagtc aagaactatt cttatctgaa ggtacaataa
    29341 agaataacat aactaatatt ttagctaaac tagaacttcg tgacagaact caactagcaa
    29401 tctttgcttt caaaaataaa attgtaatag aataaatcta aaaatataaa gtgacttaag
    29461 tcactttttt tagtgactta tagtaataga attataattc acaattcttt attataatat
    29521 tatagctttt taacttatac aaaattattt tggagggatt actatggata tagtcagagt
    29581 aaataatata acaaaaagat ttaatgataa attagtactt gataacatca gcttttctgt
    29641 taaaaaaggt gagatatttg gacttattgg cccaaatggt gcaggtaaat ctacacttat
    29701 aaatataatt acaaatctta tgttaccaaa tagtggtagt attcaaatca atggtttaga
    29761 tttatcaaaa gattatataa aagcaaagtc tataataggg ttagttccac aagaattagc
    29821 aattatagaa actcttaccc cttttgataa cttagagtac tttggagctt tctacggttt
    29881 aaagggaaaa ctacttaaag aaagaatcat tgaagcatta gaagttacag gtcttactga
    29941 agtcaaaaag aaaaaggtca aaaaattatc tggtggtatg caaaggagat taaatattgg
    30001 aatagcactc ttaaatcatc ctaaaatatt aatattggat gaaccaactg taggtgtaga
    30061 cccccaatca agaaatcata ttttcaattt tataaaagat atatctaaaa aacatgaaac
    30121 tactgtaata tatacttccc attatatgga agaagttgag cacctttgtt ctaaaatttt
    30181 tattatggat gaaggtaaag aaattgcttt tggaaataat gattatttaa aatcactcgt
    30241 atctacaaat acaaaattaa ttatggaaat aaaaaatata aatgcccaac ttatctttga
    30301 tttaaaaaat actaaaggtg ttatatctgt tttagaaaat aattcattat tagaattgga
    30361 tatagataaa aaattacaac ttacagatat actctctgtc attgacaaaa acaattcaaa
    30421 aataatgaaa atttcttatg aagaacctag cctagaagac gtatttttaa atctaacagg
    30481 aaaaaattta agggactaat ggaggtgatt ttatgaaatt atacatgagt gtaaaaacta
    30541 tgctaaagag tttaaaaagc tcctttatac ttaatttaat atatttttta gctttgcctc
    30601 tgattttatc ttggtttctt gggatggtta cagaaagcat gtttcaaaac cctataaaaa
    30661 cagaatctac ccccattgtt atatatgaca aagataacac aagattatca aatgatttga
    30721 caaagtattt gaaaaatgat ttatcttata tattaacagt taaaaaagat gattctaagg
    30781 cagaattaaa gcttactata cctaaaggct atgagagtag tttattaaat gaaaaatcaa
    30841 atactctaaa catagaaaaa cttggaactc gtgatgatat tgcaatattg cttcaagata
    30901 tattagatac ttaccatgaa aaattttatt taaataattc tcagaaaata agttcagaag
    30961 atttttctaa attatttaat aaaaattcta ttgatacttc tataataaat aacaatgtta
    31021 aacagagctc ttatgaatac tttgctttag tttcactagg atttcttgta ataattttta
    31081 taatgaacaa tattttgtca aattatataa gtgaatcaaa gggactttca aaaagacttt
    31141 attctatgcc tataactaga gtacaattct taatttatga ctttgtaggt ttatggattt
    31201 attcatttat attcttatta ttatatgttt tattctttag aatacttgga ataacattta
    31261 aaggtaattt tgccatattg ctcctacttt gtgctttatc atcttatttt atgacttcaa
    31321 tatctacctt tgtaaccagc ttttttagta aaaaatacgg tacagcaata gtttatgcat
    31381 tactcttttt acagacaatt tttggaggta tcttcagtat gataagtgat acatttacta
    31441 agcttacaag tttatctcct acctatttaa taggagaatt gtttagtaat tatgaaacat
    31501 ttaaaaccat agattcaata ggaaatttaa ttttcacatg tttaattatc tctacaatat
    31561 taattgcact tgctattgta aaggaaaaat ataaatggag ggaggtttaa taatgattag
    31621 attaactatt gcaaatataa aaagatatct aaaaaatcat actcttttaa ttaatatggt
    31681 tatgcttcct attatactaa ttttttcact taattttttt atcaacaact cgggaaatca
    31741 atcaatgtac ttttctccag tagcaattgt atctgactct tcaggaaagt atgaacataa
    31801 gttaataaat agttctaaat taaaagaaaa ttcatttaga ttaaatgaac aagataaggc
    31861 tatgaatcta ttaaaaaata ataaggtttc tgctgtattc gttttagata aaaatttttc
    31921 aaatagtata gataaattac aacgacctgt agttaaatgc tttaaaattg aaaatggtgg
    31981 tggtagtctt tgggcagaat cacaaataga atcttttatt actaaatcgt taaaattgaa
    32041 agtggataaa aatatagatg ataaattgac aaagacaaat ataattgata acaaaccaga
    32101 taagaataaa ggttcatttt tagtagtctt cttgatatgc tattttatgt atattaatgc
    32161 ggcacatctt gcttctgatt tatttacatt aaaaaaatca aatgtgctaa aaagacttat
    32221 ctctacagat aataaagata taaaaataat tttcagtata tttttaggac tattttttat
    32281 tcaatcaatt gtatacactt ttgctctatt atgtttttca attatagaag actttaattt
    32341 gagtctgaat gtattgatga ttgtactagc aaatagtttt gtttccactg gatttgtatt
    32401 ttgggtagct agagtattta aaaatgaaag ttctataagt cttatatcta cattttattc
    32461 tttaataggt ctagctatat ctatctctag tctaatacca tcaatggata aattatcctt
    32521 tatgactaat ctttcaaagt tcacaccatt ttattggact atagatgcta ttaaaaacaa
    32581 tggtagtatt ttaataaata taatagctct aatattaatt ggaattatat ttgtaactgc
    32641 aggaagtctt aaactaagag attttgctaa aaactaataa ttattaaata taattagtat
    32701 ctatttttat ataattgtaa aagaatattt caacatatta aattatttta tcttatattt
    32761 ttctctttag tttcacacat caccaagtct aaactctata ttatatatta atatttttga
    32821 cttgtcaagt tatttatgtt ataatattaa tcattagata attaaagcat tgtacctaag
    32881 ttgcaatatc taaaattcaa tttggaggta taactagcat atgtataaaa ttggtgaaag
    32941 tgtaatgtat ccaaaagagg gagcctgctc tgtaaatgat attgtaacaa agaaaataaa
    33001 tcatgagatg caaaaatact atgaattaag tgtcattttt aatagtaacc ttaaaatttc
    33061 gattccagtt ctaaatgcag atagaattgg catacgtcca gttatggatg gaaatgatgt
    33121 tgataatttt atccaatcta ttaataaaac agatggtgta tggatttttg atagaaaaga
    33181 aagacttaaa ctgtaccaag ataaatttca ttcaggagat gtttttgaaa ttgtaaaatt
    33241 aatcaaaatg cttatgatac aagacagttc aaaacaattg tgtagcacag ataaggagtt
    33301 tttaaataaa gctcaaaaat ttgctttatc agaattagct gctgctcaat gtaaatccta
    33361 tacaatggta ttagaggaaa tgaaaaaaca tatattaaat tcaaagaata caaattaaac
    33421 cagtttttta aactggtttt attttttata ctaatcatcc atgctacttt agcttatttt
    33481 aaactgccta atctaatatt aataaagtta tctcaattag atagatgtat tttattcaaa
    33541 taaaaaaaac taagagtaaa tcatattact gatttactct tagctaataa aatttaatct
    33601 tcttcaagtt cttttttctc ttgtaataat acacttaatt catttgcttc taaaacttgt
    33661 gcctctgcaa tatcaagtaa ctcttgttgc cattccacac atttatcttt gtaatcaaaa
    33721 ggtagcgttc tgtcaactat gtacttatca atacagtcct ccgcactttt aattatttct
    33781 tttctttcat caataaattt ttctaattct actaaggctt cttcataagc ttttctactc
    33841 atataaattc cccctttgta caattattct acattaaaat gatttttcct cttttttgta
    33901 taaaatttta tttgtatata acttttccat caatatcttt tatagtttga catccagtta
    33961 atatcataga actacttagc tctgattgaa gtttatttac ataagtttct acaccatcag
    34021 ttgcaccacc aaaagaagca gtcacaaaag gtcttcctat taatactgca tctgcaccta
    34081 aacctatcat ttttagtacg tctaaaccag ttctcactcc accatctgca agtattgtaa
    34141 ctttaccttt aacagcatca gcaatttttg gtaaaacttc acatgctcct ggtgtacagt
    34201 caagcactct tcctccatga tttgatacga ctattgcatc cacacctgct tctactgcca
    34261 ttaaagcatc ttcaacagtc attatacctt tcaatataaa tggtagttta gtagacttta
    34321 ctaactcctt tatttgttta acatttttag gaagtactgg ttttccatgt aatgataaag
    34381 ttactagacc acaggcatct atatcaactc caactgcaaa tgcaccagct tcttcagcta
    34441 atcttatctt ttttataata ttttcattat cccaaggttt aataaataca attccagctc
    34501 cattatactt tttgactaca tctaaatttt ccattaaaaa agcatctact gccgtatcac
    34561 caaccattgc ataaatacca ctattaacac atcctgcaac tactggttct atgtaatctc
    34621 tttcattaat ttttccaccc atatttaaag tagttcctgt tactggagct gcaaatatag
    34681 gtgcactcat tttctttcca aacatctcta tagatgtatc aggatttgat acatcatgta
    34741 ttactctcat atttactttt actttttcta aactcttgct attttctata aaagaagaac
    34801 cacttccttt acctcccatg ccaggcactt cacctgcaca agcaacccca ttacaaacct
    34861 tacaaacttt acaacttcca tttaaatttt ctctagcact ttttaataat tcattatatt
    34921 ccatctttaa tcccccatta actatagtaa tttatattat atacattata tcatattata
    34981 tttacagtat atttttcaaa aaactttttc tgtggatagg agttatgcca tatttttcaa
    35041 tagcttcata gtgttcttta gttccatatc ctttgtgaga tttaaaccca tattctgggt
    35101 atactctatc atattggtac attatactat ccctagtaac tttagcaagt atacttgctg
    35161 ctgctattga tatagatttt gaatctcctt tcacaattgg attttgagat atgtcaatac
    35221 ctggtattgt tgctgcatca actaataaat aatccggtgc tttttttaaa caatttatag
    35281 cctttttcat tgccatatat gtagcattta atatattaaa ttcatctatt tcttcattgt
    35341 ttactatacc tattccataa tctagtgctt cttcttttat tacttcaaaa agttcatctc
    35401 tttttgcctc actcagtttc ttagagtcat ttacaccttc tattttggta tcttttttaa
    35461 atactacaac agaagctaca acaggtccag caagtggtcc tcttcccgcc tcatcaattc
    35521 ctccaatata taaataacct ttatcatagc cttcgttctc aaatatattt atagtttcca
    35581 atctttcttc ttcttttcta atattatcca gcttttttgc cagttttata gctagacctt
    35641 gtacagattt tctttcatca actcttaaca gctctatata ttccatatat ttttctactt
    35701 ctaaagtctc tattatttct ttaatctccc tcacactttt atcctgcata ccattccctc
    35761 atctttcgtt tttaaaaatt taatttttaa atatattaaa attttttaat tagagtttaa
    35821 ttgcatccac tatagccaca ttttaaacat atactacatc cacctgtatg tgcaattcct
    35881 tcacttccac actcagggca cacaaatttt tcatctcctt caaaattttt atttattatt
    35941 tctttggctt tttcatcttt attatctttt tcttttttat tatttttata atattctttt
    36001 ttactatttt cttttttatc ttctttatta ttttcttcct ttttttcatt tattaaaatt
    36061 ccactccttt tacatccatc tctaaatatt gttactcctt taagaccagc ctcccatgcc
    36121 ttaatatata tattgaaaac atcttcaact gtagtttcaa aaggtatatt tattgtagat
    36181 gatatagaag catcaatata cttttgccat actttttgca tgtttattct ttcttcatat
    36241 cctatattca tagcagtaac aaaaaaatct ggtaaacatt cttcatctgt aatacctttc
    36301 ttgtccatat actctttaac aattggagta tatactttat aatacacatc ctcatcatgc
    36361 aagctttcag tttttcttat atatgaaaga ttaaatatag gttctattcc accactgata
    36421 cctatcattg tagatatgga acctgtagga ggtatggtta aaagttgtga atttctaagc
    36481 ccatactttt taaccatttc cttagtgtct tcatttagat tttctataaa aaacttagat
    36541 ttagaaatat tttcatattt atactcacta aatgttccat attctttagc aattaatgct
    36601 gattgcttca cagcttcatt taacatagtc ttaccaatta aatcacatag ttctaaagat
    36661 tcatcagaac catatcttat tcccattttt ataagcatat cagctatacc cattacacct
    36721 aaacctatct gtctatactt atgtacacta tctctttgtt cttgtaatgg atgcaagttc
    36781 aatccctcat ctaagacatc atttaaagct actacagctt ctctaacgca agattttaat
    36841 ttattaatat caaaagtagc atgttcacca aatggttgta ttataaactc tgacaagttt
    36901 attgagccaa gtagacaact tcctccactt ggaagtggtt cttctgcaca aggatttact
    36961 cctgaaaagc aaaagttctt atcttcactc ataaaattat atgtttttat tttatcccaa
    37021 aacaatatac ctggttctgc ataatcccag ttatttttca caaatttatt aaataaatca
    37081 taagcattta ctatcttaac tattttttct ccagtagttt caacttcaaa agtacaatta
    37141 tataactttt tttctcttac agcttgcata aattcatctg ttactcttac agaaatattt
    37201 gctttagtaa ccttctccaa atctgttttt atatctataa agtcttctat ctctggatga
    37261 tttacatcca tagaaatcat aagagctcct cttctccctc tttgaccaat taagccagtt
    37321 gtcatactgt aaagttccat aaaagagact gctccagtcg tatgttttgc agcattgtta
    37381 actttcgctc cttttggtct tagctttgaa atatcaattc caactcctcc accataacta
    37441 aaagttctag caagtttttt tgcagtatca aatatatctt ctatgctatc ttttggtggt
    37501 tctattacat aacaattaga atatgttact ttttttccct ctttctgtaa tcctctattt
    37561 gctaaaattc ttccagcaaa tagaaatttt ttttgtctta ttagttttga tattcttgaa
    37621 ctattatttg aaattctctt tatccattca tcaaaactct caccattatg tctatacttg
    37681 ttcttccata ttgatttttg taattcatca aactcccaag ctttagtcct tatttcagag
    37741 cgtctttctc tatataatat gtagtgtttt gctatatctt ttctatttga ttccataagt
    37801 agtacttcaa ctatatcttg tatttcttct acggataagc atttttccat agaattatag
    37861 tcattagcta tttttcttga tataccatat gccaactctc tatctactcc accttcacta
    37921 tttatactag ctttatagac agcatcttca atcttacttt tactaaaaag ttcttttgta
    37981 ccatctcttt ttattatgta tttcattttt aaacctccaa taaattagta ttaaattaat
    38041 acacacatta actataactc taaacaaact ttttatatat ttgtatattt tcatattaat
    38101 tatctaacat aatatata
//
LOCUS       DAMJVK010000033        37057 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.033, whole genome shotgun sequence.
ACCESSION   DAMJVK010000033 DAMJVK010000000
VERSION     DAMJVK010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 37057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37057
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.033"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            109..1071
                     /locus_tag="PC414_001725"
     CDS             109..1071
                     /locus_tag="PC414_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2464122.1"
                     /translation="MQAISDKFDANRKTISELRKKGNTKAKYPYKTKKFYIIPFKASA
                     INRNAKGNLKLSMSKGRYLELDFNVENIKTAEIVWRNGYYLYYTFDNELNGVVAKGTN
                     TVGVDLGEIHSIASVTNKGVGLILSNREGRSIKQFRNKMYAYISKRLSKCKKGSRQSK
                     KLWRLKNKIRSKTDNQLMNLYHQTTRKFIDFCVEQKVCEIVLGDIKGVEKDTKKKKRL
                     NRVNRQKISQMEYGRIKDYIKYKAKEQGIEVKLVKENYTSQTCPKCSKKHKPTGRTYS
                     CTCGYETHRDIVGAWNILNKKHKYGLVDFRINHKQPINLKVSTV"
     gene            complement(1455..1967)
                     /locus_tag="PC414_001726"
     CDS             complement(1455..1967)
                     /locus_tag="PC414_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2464123.1"
                     /translation="MLEVIKNRRSIRTYIDKNIEEDKITEILKSAMQAPSSKNAQPWE
                     FIIVDDKELLKQLSKSQHRAKHIEFAPLCIVVLGNRDKFLKPGKWIQDLGACTQNLLL
                     EVTNQGLAACWAGVFPKNKVVNKVRQTLDLPLKLVPYALISIGYSEEKNEFIDRFDEN
                     KIHRNVYKNR"
     gene            2417..2893
                     /gene="purE"
                     /locus_tag="PC414_001727"
     CDS             2417..2893
                     /gene="purE"
                     /locus_tag="PC414_001727"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HDF2464124.1"
                     /translation="MKVAVVMGSKSDYPKLEEGIKLLEKYGIEVVARALSAHRTPEQL
                     SIFLKEIEDDTDVIIAAAGKAAHLPGVIASQTLIPVIGLPIKSSTMDGLDSLLSIVQM
                     PKGIPVATVTIDLGLNAALLALQIMTLKYPKLKEDLKSYREEMAQKVLEDDNNLRG"
     gene            2899..3597
                     /locus_tag="PC414_001728"
     CDS             2899..3597
                     /locus_tag="PC414_001728"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDF2464125.1"
                     /translation="MLLYEGKAKQVYSTDNENEYVVYYKDDATAFNGEKKAEISSKGI
                     LNNKISTIIFEMLKENNINTHFIKSLSDREMLVKKVEILPLEVIVRNIAAGSICKRVG
                     LEEGVVFDEPIFEISYKNDAYGDPMLNDDYAVAMKLATREELKFLREETLKINELLKA
                     FFLKLNLKLVDFKIEFGKDSEGNIILADEVSPDTCRLWDVNTNEKLDKDRFRKDLGDL
                     VEGYTEVLSRMNNK"
     gene            3609..4976
                     /gene="purF"
                     /locus_tag="PC414_001729"
     CDS             3609..4976
                     /gene="purF"
                     /locus_tag="PC414_001729"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDF2464126.1"
                     /translation="MCGVLGIYSNKDVTKELYYSLYSMQHRGQESCGLALLDDGEIKY
                     KKDMGLVGDVFKENELSKLKGNIGIGHVRYSTAGGSHVSNCQPLVGSCRKRQLAIAHN
                     GNLVNANYLKDMLEEDGYMFQTNSDTEVILYILARYYKGDIVESLKVTMDYIKGAYAL
                     VIMSQEELVAVRDPHGFRPLVLGKKGDEYIFASENCAIDILGGEVIRDVEPGEIIVVK
                     DGELKSYFYSENYKPVKKSCIFEHIYFARNDATIDNVNAYEFRIKCGERLAQNETVKA
                     DMVVPVPDSGWPGAIGYANASGLKISEGLVKNRYVGRTFIKPTQEEREIAVKIKLNPL
                     STIIKGKSIILVDDSIVRGTTSKQLVKSLREAGAKEIHLRITSPPVAYSCYYGIDTPN
                     RSKLIASSNNVEEMREYIGCDSLKFLDIEGMLDATEHKSTFCKACFDGEYPVKKIDKE
                     ELLSC"
     gene            4970..6046
                     /locus_tag="PC414_001730"
     CDS             4970..6046
                     /locus_tag="PC414_001730"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDF2464127.1"
                     /translation="MLTYKESGVDIDEGNRAVDLIKGKIKGTYDGNVVGDLGNFSGLY
                     SLKDFVGMKEPVLLASTDGVGTKLKIAQMMDKHDTVGIDLVAMCVNDLICQGAKPLFF
                     LDYIALGKLVPEHIEKIVGGIADGCKMSGCALIGGETAEMPGMYGEDDYDLAGFSVGI
                     ADKEKIVSGNNVKSGDVLVGISSSGVHSNGFSFIRKIFLETYNYKMERYVEELGMTVG
                     EALLTPTKIYVKLALDVLAKHDIKAVAHITGGGLIENITRVIPKGLGLDINKKSWEKP
                     PIFKMIEGFNAVDERELHKSFNMGIGLVLIVDKENADDVVNFINNRENDNADYVDKKY
                     SELLEDKAYIIGEVVDSHEGVELC"
     gene            6040..6633
                     /locus_tag="PC414_001731"
     CDS             6040..6633
                     /locus_tag="PC414_001731"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDF2464128.1"
                     /translation="MLNIGVLISGGGTNLQAVIDGTESGEIKGQVKVVISSKQGAYGL
                     ERAKNHNIKAICETDEDKIIEILKENKIDLVVLAGYLKIISPKLVNEFRNKMINIHPS
                     LIPSFCGAGFYGEKVHQGVIDYGAKVTGATVHFVDEGADTGPIIMQDVVKVNQDDDAK
                     TLAKRVLEVEHRILKESISLFCENKLKLQGRRVFINE"
     gene            6626..8158
                     /gene="purH"
                     /locus_tag="PC414_001732"
     CDS             6626..8158
                     /gene="purH"
                     /locus_tag="PC414_001732"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HDF2464129.1"
                     /translation="MSKRALISVTDKTGVVEFAKELNKLDYEIISTGNTFKTLKENGV
                     NVMQVEDVTNFPEILDGRVKTLNPYIHGGILYKRDKESHVETVNEHKIHSIDLVAVNL
                     YDFEGTLKAGKSHDEIIENIDIGGPSMIRSAAKNYKDVIVVVDIKDYDSIIEKLKTDT
                     MTLEDRKKLSYKAFSTTGRYDALISSYFAGEVGDTYPDILNLTFQKEQTLRYGENPHQ
                     NGFLYSQSNAKNPILNYEQLGGKELSFNNLNDLHGCLEVMREFKDSEEVVSVAIKHAN
                     SCGVGLGKDAFEAYTKCYEADKVSIFGGIVGITSTIDKATAEKLNEIFLEIVVAYDFE
                     PEALEILKQKKNLRILKLAKIENSLQPYEMKYLDGKLLIQDRNNILAEKSENVTKEKP
                     TDAQLKDMEFGMRVVKNMKSNAIAIVKNGQTLALGCGQTSRIWALKNALENNKDKDFT
                     GAVLASDAFFPFDDCVTLAHEYGISAVVQPGGSIKDKDSIEACDKYDMVMVFTGIRHF
                     KH"
     gene            8169..9419
                     /gene="purD"
                     /locus_tag="PC414_001733"
     CDS             8169..9419
                     /gene="purD"
                     /locus_tag="PC414_001733"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HDF2464130.1"
                     /translation="MKILVVGGGGREHAICWKLSKEKNVEKIYCAPGNAGIANVAECV
                     NIGDTNIEELLKFAKENEIGLTIVGPEVPLVMGIVDEFEKEGLRVFGPNKKCAQLEGS
                     KAFSKEFMIKHNIPTAKYKEYTNLEEAISEIDSFGYPVVIKADGLAAGKGVVIPENRE
                     DAIATLKEMMSDKKFGAAGDKIVIEEFLKGIETSILAFVDNDTIVPMASAKDHKKVNN
                     YEQGPNTGGMGTFSPSEIYTEELANKVKETVLEKTLEGFKKDGLNFKGILFVGLMITE
                     DGEKVLEYNVRFGDPETQSVLFRLETDLHEIMEAILDNKLKDIEINYSDDEAVCVMLT
                     SGGYPDSYEKGKIITGLENLDDDIVVFHSGTKMFDGNLVTNGGRVIGITAKSTTVKDA
                     AEKVYENIKKINFEGMHYRTDIGR"
     gene            9444..13250
                     /locus_tag="PC414_001734"
     CDS             9444..13250
                     /locus_tag="PC414_001734"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HDF2464131.1"
                     /translation="MLNTENKDSMVRRVLVEKREGFDLEAKALKKDLVESLHIDNIEN
                     LRILNRYDVEGISEEVYENAAKTIFSEPNLDVVYYEEIPKLNDERVFAIEFLPGQYDQ
                     RGDWAAQCVQIVNQGIRPAINTAKVYILSGKITDEEFSKIKDYCINPVDSREASLEKP
                     ETLKMETEIPTTVEVLDGFIDLDENGLKTFVSEKGLAMTLGDLQHVQKYFKDTEKRNP
                     TITEIKVLDTYWSDHCRHTTFMTEIENVKIEDGKFNDIVKEAYQMYLNSRDNVYVNRH
                     KDICLMDIATVAVKELKKNGKLNDLDESEEINACSINVDVEVDGKMEKYLVMFKNETH
                     NHPTEIEPFGGAATCLGGAIRDPLSGRSYVYQAMRVTGSADPRTTLEDTLPGKLMQRK
                     ITTEAAHGYSSYGNQIGLTTGQVAEVYDENFVAKRMEIGAVIAAAPKENVVRERPEAG
                     DVIVLLGGKTGRDGCGGATGSSKEHSEESILTCSAEVQKGDAPNERKIQRFFRNKEVA
                     QMIKRCNDFGAGGVCVAIGEIADSLDINLDLVPKKYDGLDGTELAISESQERMAVAIK
                     KENKDKFIQLAVEENLEATHVATVTDTGYLRMFWNGKAIVDINREFLDTNGVKQTTDV
                     HVTKVDEENTFFSSNEIVKDVKCASMKDKFTKVLSDLNVCSQKGLVEMFDNTIGGNTV
                     LMPFGGKYQATPTQGMVAKIPVLGGETNTSTIMTYGYNPKVGKWSPFHGALYAVVESV
                     CKLVAIGGNYSTTRLTFQEYFEKLGNNPEKWGKPFSALLGAFYAQSKFEIPAIGGKDS
                     MSGTFKDIEVPPTLVSFAVDTVDAKKVVSPEFKKADSKVVMLCVNKAENDVVDFEELK
                     RNLDKVRELIHGNKVLSTYALGFAGVGEAISKMAFGNKIGFKFSEEAEKAFTDDKLFE
                     ASYGNIVLELANDDLSMLEGYNYVVLGSTVKETSIFIKGEELALDELYKAHCSTLEPI
                     FPTKTEEVKSKIETISYISQGEAKKSSLSIATPRVFIPAFPGTNCEYDSARAFERAGA
                     NASIRVFKNLTYKDIEDSIDTIVNEIKSSQIIMLPGGFSAGDEPDGSGKFIATVFRNP
                     RVQEAINEFLTQKDGLMLGICNGFQVLIKLGLVPYGEIRVPSESAPTLTYNNIGRHQA
                     KIARTRISSNKSPWLAQTNVGDIHNIAISHGEGKFVASEDVMRELIANGQVATQYVDF
                     NNEATYDIEFNPNGSFYAVEGITSADGRVFGKMGHSERIGEEVYKNIIGEKDQKIFES
                     GVKYFR"
     gene            13456..14328
                     /gene="rfbD"
                     /locus_tag="PC414_001735"
     CDS             13456..14328
                     /gene="rfbD"
                     /locus_tag="PC414_001735"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HDF2464132.1"
                     /translation="MKILITGSNGQLGKELVNQLEAINQSINQPKYVILATTRSDLDI
                     SNQTNVDNFILHNKPDVVVNCAAYTKVDACEDNIEIAYKINALGVRNLAIASEKVNAK
                     LIHISTDYVFNGFSKYPYREDNKTEPNSVYGKSKLMGEKFVEQFSHKYFILRTAWLYG
                     NGNNFVKTMIKLSLENKEVNVVNDQFGTPTSTVDLAKIIIKIMETEHYGVYHATCEGE
                     CSWYDFAKKIFKLKNIDIKVNPIKSNEFKCKAPRPLYSVLDNFMLKLIGLNSFRKWEE
                     SIEEYLERYDINEK"
     gene            14437..15306
                     /gene="rfbA"
                     /locus_tag="PC414_001736"
     CDS             14437..15306
                     /gene="rfbA"
                     /locus_tag="PC414_001736"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HDF2464133.1"
                     /translation="MKGIILAGGSGTRLYPITKCISKQTLPIYDKPMIYYPMSVLMLA
                     GIQDILIISTPRDIKAFEELFGDGSSLGLNITYAIQEKANGIAEAFIIGEEFIGNDRV
                     SLVLGDNIFYGYGFSERLENAVKRKGATIFGYHVSNPNSFGVVEFDKDFNVLSIEEKP
                     TVPKSNYAIPGLYFYDNDVVEIAKLVEPSDRGELEITSINSEYLKRGKLKVELLGRGM
                     AWLDTGTHKGLLDASNYVEVVQTRQGLYIACLEEIAYTKGYISREQLMKLAKPLIKTE
                     YGQYLLKLASEDM"
     gene            15355..15912
                     /gene="rfbC"
                     /locus_tag="PC414_001737"
     CDS             15355..15912
                     /gene="rfbC"
                     /locus_tag="PC414_001737"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HDF2464134.1"
                     /translation="MGKINVIKTKIEGLYIVEPNVFYDERGYFMESYNKKDFIEQGLD
                     MCFIQDNESSSRKGVLRGMHFQVKYPQGKLVRVTEGEVFDVAVDLRKDSQTFGKWEGV
                     ILSDKNKKQFYIPEGFAHGFLVLSEKAVFSYKCTNYYYPEFENGFIWNDKQISIEWPL
                     KGIEEIFLSEKDKKQKSFKDICTRR"
     gene            15950..16933
                     /gene="rfbB"
                     /locus_tag="PC414_001738"
     CDS             15950..16933
                     /gene="rfbB"
                     /locus_tag="PC414_001738"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDF2464135.1"
                     /translation="MKKILITGGAGFIGSNFIHYMLNKYVDYFIVNLDLLTYAGNLET
                     LIEIQDNSNYKFVKGDISNREQVYKLFEEERFDIVVNFAAESHVDRSVENPDLFIKTN
                     IIGTQVLLDASIKYGVKRYHQISTDEVYGDLPIDRKDLFFTEQSSINPSSPYSASKAS
                     ADLLVSSYYRTYGLLTTISRCSNNYGPYHFPEKLIPLMILNALENKQLPVYGNGENVR
                     DWLHVYDHCTAIDLIIHKGNIGEIYNIGGHNERSNLEVVKMILNLLGKSEELISYVND
                     RPGHDLRYAIDATKIENELGWKAKYDFDLGIKETVKWYIENESWWKAVLSV"
     gene            16963..17631
                     /locus_tag="PC414_001739"
     CDS             16963..17631
                     /locus_tag="PC414_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HDF2464136.1"
                     /translation="MNEISSLINILALTQLSGSNTTNQCNCTYNNSSGFDMIMMTLLK
                     ALSQNNQHTSNSYNLLSNSENLFNELDNVVNNTTSRFIDVDTKDKNVKLRIENAVNQA
                     SKKYNVDSNLIKAIIKVESDFNPNTVSSAGAKGLMQLMPENCRDLGVTDPFNIEQNID
                     AGTRHIKEYIDMFGGSIEMGLMAYNGGPGRMKSRGVESISDLYKMPKETQNYIPKVMK
                     YYRG"
     gene            17640..18068
                     /locus_tag="PC414_001740"
     CDS             17640..18068
                     /locus_tag="PC414_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaR family protein"
                     /protein_id="HDF2464137.1"
                     /translation="MGINIDKLNISPSINMEVKKLKESNKFSDKLNSLKQSSSLEDIK
                     RNLESVKKAGERLVLTQNYGDISIYKNAVKEYLKSVVDNMYSLDKNSSFWEHQYYKNV
                     KIVDSKLEDMTSKLISEEKENIDIVSTVDMIQGLLIDMYR"
     gene            18092..18406
                     /locus_tag="PC414_001741"
     CDS             18092..18406
                     /locus_tag="PC414_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HDF2464138.1"
                     /translation="MNSKKEDIYELNFGQLIEEKSGVIEENQNVFNAKVKVAVSIGNT
                     TETIKNILDLKEGSIIKLSKNVDEKLDIYANDRLFAYGESIITNEKLSVRLSKIEDSD
                     VN"
     gene            18557..18832
                     /gene="flgM"
                     /locus_tag="PC414_001742"
     CDS             18557..18832
                     /gene="flgM"
                     /locus_tag="PC414_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HDF2464139.1"
                     /translation="MDIKNVSSNIVVNNYLKNTSGIKKVEEVKKINKTDKIELSKFAK
                     EMASVSNMSDENIRNKKVDEIKNALENGTYKIKPKEIAKKMIEDMRG"
     gene            18837..19253
                     /locus_tag="PC414_001743"
     CDS             18837..19253
                     /locus_tag="PC414_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HDF2464140.1"
                     /translation="MITDLKVIIYEEKNIISKMMELLKEQFDYIVEKDLENLNMLNPK
                     LEDISRELASIEIKRRQLFGNDISMSEVVENSNDGYLKEIYTDLKRILALAKNQQESN
                     DSLIKKELIFTKKMINFIKPADKQTTTYNSYGNIRK"
     gene            19271..20581
                     /gene="flgK"
                     /locus_tag="PC414_001744"
     CDS             19271..20581
                     /gene="flgK"
                     /locus_tag="PC414_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HDF2464141.1"
                     /translation="MAGLFSILNTANSGMNAHQKAIQTISHNISNLDTDGYSRQRTEF
                     ATNSPMYMPSLSNSIARGQLGTGVHVTDVTRARNSFYDYQFRAEAHKYGKTVSKYDYY
                     NTIETILNEPSDYGISAGIDDFFKGWESLSKDINSDSKKSLVVENASNLATLISESYK
                     KLDKLKEDIDSNIDTEIKQINDMLKSLEDLNKSIDIISGSGSTPNDLLDERDRILDNL
                     SFKLDFENSDVKNMLADGKLELNELKNPDGTWKTGISGTLQGLFEMHGKIDTYKSDLK
                     DVSDGLAKQINDVYNTSAGITVTDFFITSNVDGEDIIKINPAIKSNPNELKLTADEAS
                     KIAKLKDEKIDIGVAGGKVSTISDHYKAFAESIGLDSQKVNQDEANQRKIINNIDNSR
                     MSVSGVSLDEEMTELIKIQRSYQASAKVMSTAVQLLDVVINGII"
     gene            20600..21532
                     /gene="flgL"
                     /locus_tag="PC414_001745"
     CDS             20600..21532
                     /gene="flgL"
                     /locus_tag="PC414_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HDF2464142.1"
                     /translation="MRVSTGMMSSSYLNSLQDNLQRLDKVNRQVNTTKEINKLSDNPY
                     KAIKILNSKSEIKTMETYVENCKDTGNWLETTDTALDQLGKLLSDIKKTLVASGDGAY
                     SDDELKTLSNETNEKMKEIANALNATHEGKYIFSGSNTGVPPVECIDNADGSVSLKFN
                     SSLNLNKLNDSLSMDIAQGISVDYNVKLSSLGFDPTKTDPFEELNNISKKLANPDADA
                     IKELTTTCLGDMEQLIENTVNVRSIYGTKANTVEAMKEKNDDSLIQLKDILSQNEEID
                     YGEKLVQLKAAELTYQASLQTGGKLFNVSILDYI"
     gene            21548..21940
                     /locus_tag="PC414_001746"
     CDS             21548..21940
                     /locus_tag="PC414_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HDF2464143.1"
                     /translation="MMKVTLKKGILGFENLKEYELLDIEDNDYLKEFNSTEEEGIGFV
                     VVSPFEIINEYEIVLNQETIEKLEVKSPKDVVLFNIITLGQTFEESTVNMKAPIVINI
                     KTNFGMQIILQDDKYSIRHPLLRGDNGC"
     gene            21934..22146
                     /gene="csrA"
                     /locus_tag="PC414_001747"
     CDS             21934..22146
                     /gene="csrA"
                     /locus_tag="PC414_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HDF2464144.1"
                     /translation="MLVISRKKDEAVLIGESIEVKIVGVDGNNVKLAISAPNNISILR
                     KEIYEKVKSENIKATNKNIKILKSLK"
     gene            22168..22563
                     /gene="fliS"
                     /locus_tag="PC414_001748"
     CDS             22168..22563
                     /gene="fliS"
                     /locus_tag="PC414_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HDF2464145.1"
                     /translation="MYGENPYNSYKQNAVFMASKEQLLLMLVDGAVKYTKIARAAILD
                     KNTQKAHRELVRVQDIFTELMVTLDQNAGQWAKDMYKVYDFVRYELSRANIRKDIQVI
                     DNVLPVIEQIKDTWHEADKKSKEERSRYK"
     gene            22563..22919
                     /gene="fliS"
                     /locus_tag="PC414_001749"
     CDS             22563..22919
                     /gene="fliS"
                     /locus_tag="PC414_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HDF2464146.1"
                     /translation="MELNLAELDKLSKEELLLMLVDGTVKYTNISKEALLNNDYLKAH
                     NELVRVQNIFTELMTTLDQNAGQWAKDMYKVYEFIKSELAIADENKDIKIIDDILPIV
                     KQIRDTWHEVYNQLKI"
     gene            22940..24463
                     /gene="fliD"
                     /locus_tag="PC414_001750"
     CDS             22940..24463
                     /gene="fliD"
                     /locus_tag="PC414_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HDF2464147.1"
                     /translation="MSSISPIRVTGLSGNFDMEGIIEASMIRDKEKVDKAKQEQQIVK
                     WKQEIYRNVIQESKDLYDKYLSINSPNSIVSEKAYSSTRITSSDESIIVAKGSAGAEK
                     INYQFAVSQMAEPAKFTIKLNSSEPIVRQFPPNASGASSLTIGDVNIPISEQDTTSTI
                     VSKINSLCADNDIKASYSEMTGELIISRKQTGSSSDINLRVIGNDNLAQQIANDNGIT
                     FINDAGGNKVANVYGKNLEADVTDEHGRVTHISKEQNSFNIDNIDYNVNSKGTAKLTS
                     VTDTEEAVKNMQAFVDDYNKLMDKVYGLVTTKKPKDYPPLTDAQKEDMTTEEIEKWEK
                     KAKEGILRNDDELRGFVEDIQSAFFGDGKNIIALRKLGINESENYNKKGQISFNADTF
                     SKALIDDSDKVYKTLAGYSSNYDDKGMFEKLKDIVYEYSGSSTSKLPKKAGIEKTASA
                     SENVYSKQIAEQERNISRLVEKMNDKEKRLYAKYSALESLLNQYSSQMNYFSQAQGN"
     gene            24466..24801
                     /gene="fliT"
                     /locus_tag="PC414_001751"
     CDS             24466..24801
                     /gene="fliT"
                     /locus_tag="PC414_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HDF2464148.1"
                     /translation="MDKLKEKLNLYKDISLQIINLIEKEEYIKISIKLGERQEIINSV
                     SEIDRNDFIQLYNRMELIEIDSRIRDILQGQLLEVKKELHEYKLTKQVNTMYYNLNRE
                     KVNIFNKKV"
     gene            24903..25775
                     /locus_tag="PC414_001752"
     CDS             24903..25775
                     /locus_tag="PC414_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HDF2464149.1"
                     /translation="MRVNTNVSALIANNQMGRNVNAQSKSMEKLSSGVRIKRAADDAA
                     GLAISEKMRAQIKGLDQAGRNVQDGISVVQTAEGALEETGNILQRMRTLSVQSSNETN
                     TAEERQKIADELLQLKDEVERISSSIEFNGKKLLDGSSTEIRLQVGANFGTNVAGTSN
                     NNNEIKVALVNTSSIMSKAGITSSTIASLNADGTSGTNAAKQMVSSLDVALKELNTSR
                     AKLGAQQNRLESTQNNLNNTIENVTAAESRIRDTDVASEMVNLSKMNILVQASQSMLA
                     QANQQPQGVLQLLG"
     gene            25868..28009
                     /locus_tag="PC414_001753"
     CDS             25868..28009
                     /locus_tag="PC414_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDF2464150.1"
                     /translation="MLLSIVMIVKNEEKILEKTLKSLTTLRNSIESELIIVDTGSTDD
                     TIKISKKYTEKVYFHNWNNDFSSMRNISISYAKGEWLLILDADEILIDSSTIVKFFKD
                     GLDKKFNSASIQLKNLYSYDKKLYGYCSVLRLFKNVDFRYHGKVHEQPIYKNPIFNNV
                     ADFEHYGYLFEDEEIRLNKVKRNEKLLFQELKENDNSPYTNYQLGKNFIILGKYQEAL
                     YYLEKSYELYSKLELAPGYLITSLVKTYLYLGKHKKCERLCLKYIKKDMNNIDIYYYI
                     AQAQVDLGKYENSIDSYKRYIYLLDNYEISTQANSLFSDTDTVGLRDEAIITLIKIYY
                     KLEKYEFVISEYNNIEDVEKKKNVYFSLFMSLYKLNRFEDIKNYYKEVSNSKIEKNSF
                     YRNIEEVINNIKEDEKEYVYKILCDIEGNYGILNQIRINKCVSIDKCKEILNEEKEII
                     YAYIIKIAFEEKIDLLDIFYEMEYVWIEKYLKYLLALERSFNLVLYKYIISRPNTSDM
                     EKIRVYKAISKVVLESISLSDEKYKEIFYLYIMYSYKYIRYIYSNLSDFELIKFVCND
                     LDKFTIEFKHLLDIKNKVNNANEKLEYIHSMRKLLNEYPFYNKIIKILIGELEENIKE
                     SEEFKDLKDSFIISIENMIETGKINDAKLLVDDYSKTFVDDIKILNIKGILYMFENKL
                     DKADFMLKKALSLDVENEDTIYNIEYLKSLR"
     gene            28033..29910
                     /locus_tag="PC414_001754"
     CDS             28033..29910
                     /locus_tag="PC414_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDF2464151.1"
                     /translation="MNIPIVVNEKSKDIIKDWQYTCTNEDIKFTSIIILTYNQIEYTK
                     LCIESIRKFTPKNKYEIIIIDNNSSDGTVEWLKGQSDIKSVYNNKNLGFPKGCNQGIE
                     LATGENILLLNNDVIVTPNWLYNLNKALWSQDNIGAVGPITNACSYYQQIDVDYKSIE
                     DMFIFAHKINKSNINLWKYRLKLVGFCMLIKKTVLDNVGVLDELFTPGNFEDDDLSFR
                     MIESGYKLLLCQDTFIHHFGSVSFKENRNKYTDIMYINRRKFETKWGFNPEYSGMIRT
                     DLISKIDDSLEANINVLEIGCGIGATLLEIKNIYKNASIYGIEISESAGKLAKYSADV
                     LIANIEKVKLDYKKDFFDYIILGDVLEHLIDPWKIVNELKQYLKKDGYLVSSIPNIMH
                     VSVLRNLVLGNFTYEDSGILDKTHLRFFTLNEITKMFNDSNYIINSITATSMPLSEID
                     NSFIDTICKESCKEMRQQYESYQYIVKASKILDTKRYSSISMINLKYALMRIDNELDV
                     DSNLSYIFDNYNITEPCFVDDIEYLIDTFVINKATLLNRLGVEAFNRGLGDFSINMFM
                     TVLEMDRDDIDSIYNITSVLCELEEFDTAYNLIKNSSEKVQSESDIQEILCFIEGKKY
                     E"
     gene            29903..32017
                     /locus_tag="PC414_001755"
     CDS             29903..32017
                     /locus_tag="PC414_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDF2464152.1"
                     /translation="MNNDKICFITCVNDERLYEECLVYINSLDIPKNFSIETVALRGA
                     DSIASAYNKAMRETDSKYKVYLHQDTFIINKNFIHDILEIFKKNTDVGMIGVAGVKDM
                     PVNGIWWDSDKKAGKVYDSHTGKMDIYEFSNFEECFNEVMAIDGLIMITQYDVFWRED
                     KFDGWHFYDLSQSMEFRQNKYKVIVPKQNKPWCMHDSGVVNINNGYDEYRIKFVKEYI
                     NLDLYRENFYEFDKKKASTILKSNKHVMDCMKEELTEQLRLKNYKDVLGIVSKIAHFS
                     VFNHTGLYSCEECERALLECANNLPEIENTKLFTKKDLKRRKVLHVLSEGYSTGGHTR
                     LVKNWIEIDKDSVHSVVTTWQSDTLPDWLLKAINDSGGEVISLDLISDDYLKRAVALR
                     KIAYDWADIVILYVHMWDTIPVVAFGVDGKIPILHVNHYDSTFWLGTSIADIVIDFSE
                     ASQLITLDKRGIDKSEILPMPLKEKEVFSKSEVRKRYNLEDDVTIILTIASERKYNSV
                     NDINYFKIAKTLIENTDNTIIIVVGVDTENLYWKTLSNVTDNRVICFGTTANIDDFYK
                     ISDIYMESFMVGSHTSKLDAILYDLIPVKFKNEVSPIFTNMWNFIDKFDYKNIKEVID
                     MIKRYNTSEFDRDRIKEKQSEIKHFVIDKHCIEIKNYIDKIYNEANHHKVKYDLYKND
                     EIEDYQLFVALYGYMVKNKNMS"
     gene            32026..33021
                     /locus_tag="PC414_001756"
     CDS             32026..33021
                     /locus_tag="PC414_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HDF2464153.1"
                     /translation="MNNIHINKFEKSCERNWDYFVENKSINGTFLQTRNFLNYHEEGK
                     FTDHSLIICKGEEILAVVPACETYEDGERIFFSHLGSTFGGIIIRSGFNDIRHVNAIT
                     NKLEIYLREQNFDKVYLKNTSRIFCKEDTSLLEYFLFKNNYMFYDELSMFLELSKYKE
                     DILANFKYSKRRDFKYSLKNNLTFKRLYSDEDIVCFFNLLSNCLDSKGAKVIHSVEEL
                     LEFKNNRLKDIVEFYAVYIDDIMICGSMVFNFQERVYHTQYLASNSTYNKYYPMIFLY
                     YNLIKDALERNFKYLSFGISTLNRGKYLDEGLATFKEGFGSSAICNRTYYKNINK"
     gene            33113..34345
                     /locus_tag="PC414_001757"
     CDS             33113..34345
                     /locus_tag="PC414_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HDF2464154.1"
                     /translation="MKRLMILGSIKYFENIVISAKKEGIYTIVCDNRIDTPAKKICDE
                     AIDVDVFDFDKIKSIAIDKKIDGILTGFTDSLMKPYVYIANELNLPCVISCNQLESVT
                     NKAIMKKYFKNNAIPTTDYCIIESLKDLEKIQGLNFPLVIKPVDGYGSRGVYFVSNIE
                     ELIEKFEYSKEASQDGQVIIEEFYESEEIQGLAWVHNGESHVFYIGDRELVNIHTGRA
                     GKPDRLLYPSKYCFQYEEEIKSIYQNITQAFDIKNGPLYVQMLVGAYGIKVSEVMPRL
                     PGGCDYMAISQITDLDIGSLFANFSVNNEVNFDEIKKYNMHLSKCVYALPIYIRPGTI
                     KKINNIDKIENLDYVTQFLLLVKEGDTIELYEDERQDCGRVYGIANNIFDANIKKKCI
                     HEMIEILDENNENMIENF"
     gene            34365..35309
                     /locus_tag="PC414_001758"
     CDS             34365..35309
                     /locus_tag="PC414_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase"
                     /protein_id="HDF2464155.1"
                     /translation="MKLITHEQIMSLNISQKQGYEWIKESFLTKKDCQLPPKISMVEE
                     GHVFYNVMPSLNHNHMIGGIKMVTRLPGRNPSLNSEIFLYNMEDAKLKAILDGNYITA
                     FRTAAVAALAIDTFAKKDFNTIAIMGLGVTASAIMNMLSYTLKDKNINIKLLKYKDQA
                     ELFVKKYEKNEQFSFEICDTHEELIVDSDVVISTVTYTDEYLSEFKWFKKGALLLPVH
                     VMGFGNVDYLFDKVYLDDYNHLKHLENIDRYKYSCEFYDVLSKQAKGRENDDERIISY
                     NIGLSIHDILFANKIYELCETLSCEEIELKEPKEKYWI"
     gene            35403..35810
                     /locus_tag="PC414_001759"
     CDS             35403..35810
                     /locus_tag="PC414_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxcM-like domain-containing protein"
                     /protein_id="HDF2464156.1"
                     /translation="MDKYNLIRFVSIGNEYIGNLVALESNKVIPFDIKRVYYIYDVPN
                     NVKRGFHAHKNLEQVLVCLNGSVKIKCYDGVKETIFNLHKNDVGLYIGTGVWHEMFDF
                     DEKTVLISIASEHYNEEDYIRDYKEFLKYLNNV"
     gene            35897..36988
                     /locus_tag="PC414_001760"
     CDS             35897..36988
                     /locus_tag="PC414_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HDF2464157.1"
                     /translation="MLVKFNNLLKQFQTHKEEYEQKIKDVMESGYYILGNEVKHFEEE
                     FANYVGSKYCVGLASGLDALIIAFRALGISKGDEVIVQSNTYIASVMGISINGATPVF
                     VEPNEYYNIDSSKIEEKINSKTKAILVVHLYGQASNMKDIQELCDKYNLYLIEDCAQS
                     HGAKYDGKMTGTFGDIGCFSFYPSKNLGAFGDAGAIVTSNEEIADKVKILRNYGSEKR
                     YYNKFVGYNSRLDEIQAGLLRVKLKYLNEIEIEKSKIVDRYLNEINNPKVILPKIENN
                     CTHVWHLFVLQVEKRDTFMEYLKKSGIESIIHYPIPPHLSEAYAYLGYKKGSFNIAEN
                     YSDKVVSIPLFIGMEDEEVSYIIKIINEY"
BASE COUNT        15212 a         3732 c         6738 g        11375 t
ORIGIN      
        1 tacatagtta acttatctaa agaatactat tttgaacata aacaatggat taaaaagaat
       61 gatatacaaa agttaatcaa aggtaagtac aatcttcata gtcaaactat tcaagctata
      121 tcagataaat ttgatgcaaa tagaaagact atatcagaat taagaaaaaa aggtaatact
      181 aaagcaaaat atccatacaa gactaagaaa ttttatataa taccttttaa agctagtgct
      241 attaatagaa atgctaaagg caacttaaaa ttatcaatgt caaaaggcag gtatttagaa
      301 ttagatttta atgttgagaa tataaaaact gctgaaatag tatggcgaaa tggatattat
      361 ttatactaca cttttgacaa tgaattaaat ggagttgtag ccaaaggcac taataccgta
      421 ggtgtagact taggggaaat acattcgata gctagtgtaa caaataaagg tgtagggctt
      481 attctatcta atagagaagg cagaagtatt aaacaattta gaaataagat gtatgcttat
      541 atatctaaaa gattaagtaa atgtaaaaaa ggtagtagac aatcaaagaa gttatggaga
      601 ttaaaaaata agataagaag taaaacagat aatcagttaa tgaatttata tcatcaaaca
      661 actagaaagt ttatagactt ttgtgtagag cagaaggtat gtgaaatagt tcttggggat
      721 ataaaagggg ttgaaaaaga tactaagaaa aagaaaagat taaacagagt taatagacag
      781 aaaatatctc aaatggagta tggtcgaata aaggactata taaaatataa agcaaaagag
      841 caaggtattg aagttaagtt agttaaagaa aactacactt ctcaaacttg cccaaagtgt
      901 tctaaaaaac acaaaccaac tggtagaact tatagttgta cttgtggtta tgaaactcat
      961 agagatatag ttggtgcttg gaatatttta aataaaaagc ataaatatgg tttagtagat
     1021 tttagaataa atcataagca accaataaat ttaaaagtat ctaccgtttg agccatgtgt
     1081 gggcgaataa ctttaaggta gtagtggcga agacaccgtc cgaagtagct tggctttgta
     1141 accacctctg ttgtactgtt taagtataaa taagtgtgtt agccaccaag aatccccttg
     1201 ctttagctat ggggagtgtg tcaataaaat actttaatga aaaatagcta actttaattg
     1261 taggatattc ttaatatatt tgaaataaat aaaaatattg aaggatataa tattcaactg
     1321 gtatataaat aataaatgat agttaaattt taccttagag gctaattagg tgtttagtca
     1381 aagctaatgc ctttagaata caaatgtatt ctaaaggcat ttttactatg tattttcatt
     1441 tatattacta aacattatct atttttatat acatttctat gtattttatt ttcatcaaat
     1501 cggtctataa actcattttt ttcttcacta tatccaatag atataagtgc ataaggaact
     1561 aattttaatg gtaagtctaa agtttgtcta actttattta ccactttatt tttaggaaat
     1621 actcctgccc aacaagcagc tagaccttga ttagtaactt ctaataataa attttgtgtg
     1681 caagcaccta aatcttgtat ccatttacca ggttttaaga atttatctct atttccaagc
     1741 actactatac ataatggtgc aaattctata tgctttgctc tatgctgtga ctttgagagc
     1801 tgtttcagca gttccttatc atctactata ataaattccc aaggttgagc attttttgat
     1861 gaaggtgctt gcatagctga ttttagtatt tcagttattt tatcttcttc tatattttta
     1921 tctatatatg ttcttattga acgtctattt tttataactt ctaacataca gattctcctt
     1981 taaataaaat tatttctatt tatataatat cattacttta aattttagct attacttata
     2041 taaagaataa actttgtgtt ggttttatct aagtaaaagt aacattcaga actaaaaaac
     2101 aagactataa atccaaagta ttaattaaaa aagaagtaat aaaaaataat atacaagatt
     2161 taaaaaagag tattgggctt aaaatttaat tagaaaacca attttgagat aatctgtttt
     2221 ttattttaac aaaaaaacat aaataaagtt cgatacgtag cgaatgaaaa aattacaaga
     2281 aatatttttt ttaaaaaatt tcaaaaaaga gttgataata tatgtatatt aatataaaat
     2341 ttaaggtgta taattcgtat aataatacga atgatttgta gtgtaaaata acttataaac
     2401 ggagagggga agtctaatga aagtagcagt agttatgggt tcaaaatcag attatccaaa
     2461 attagaagaa gggataaaac tgcttgaaaa atatggaata gaagttgtag caagagcatt
     2521 atcagctcat agaacaccag aacaactttc aatattccta aaagaaatag aagatgatac
     2581 tgatgtaata atagcagcag caggaaaagc agcacactta ccaggtgtaa tagcttcaca
     2641 aacattaatt cctgtaatag gattacctat taaatcatca actatggatg gactagactc
     2701 acttttatca atagttcaaa tgccaaaagg aatcccagtt gcaacagtta caatagactt
     2761 aggtttaaac gcagctttat tggcattaca aataatgact ttaaaatacc caaaattaaa
     2821 agaggatttg aaatcatata gagaagagat ggcacaaaag gtgttagaag acgacaataa
     2881 tttaaggggg tagcagaaat gttattatat gaaggaaaag caaaacaagt atattctaca
     2941 gataatgaaa atgaatatgt tgtttattat aaagatgatg caacagcatt taatggagaa
     3001 aaaaaagcag aaatatcttc aaaaggtata ctaaataata agatatcaac aataatcttt
     3061 gaaatgttaa aagaaaataa tataaatact cactttataa aaagtttatc agatagagaa
     3121 atgttagtta aaaaagtaga gattttacca ttagaagtaa tagtaagaaa tattgctgct
     3181 ggttctatct gtaaaagagt tggtcttgaa gaaggtgtag ttttcgatga gccaattttt
     3241 gaaataagct acaaaaatga tgcttatggt gacccaatgc taaatgatga ttatgcagtg
     3301 gcaatgaagt tagcaactag agaagagtta aaattcttaa gagaagaaac tttaaaaata
     3361 aatgagttac taaaagcatt tttcttaaaa ttaaacctaa agttagttga ctttaaaata
     3421 gaatttggta aggattctga aggaaatata atattggcag atgaagtatc accagacact
     3481 tgtagacttt gggatgtaaa tacaaatgaa aagttagata aggacagatt tagaaaagac
     3541 cttggagacc ttgtagaagg ttatacggaa gtactttcaa gaatgaataa taaataggag
     3601 gcaaatgtat gtgtggagtt ttagggattt actctaataa agatgtaact aaagaactat
     3661 attattcttt atattctatg caacacaggg ggcaagaaag ctgtggttta gctcttttag
     3721 atgatggaga aattaagtac aaaaaagata tgggattagt tggagatgtt tttaaggaaa
     3781 atgaattatc taagttaaaa ggaaatatag ggattggaca tgttagatat tcaacagctg
     3841 gaggaagtca tgtttctaat tgtcaacctt tagttggaag ttgtagaaaa agacaattag
     3901 ccatagctca taatggaaat ctagttaatg caaattattt aaaagatatg ctagaagaag
     3961 atggatatat gttccaaact aattcagata cagaagtgat tttatatata cttgctagat
     4021 attacaaagg ggatatagta gaaagtttaa aagtgacaat ggattatata aaaggtgcat
     4081 atgctcttgt aataatgagt caagaagaat tggttgcagt tagagaccca catggattca
     4141 gacctcttgt acttggtaaa aagggtgatg aatatatatt tgcatcagaa aattgtgcaa
     4201 tagatatact tggcggagaa gttataagag atgtagagcc aggagagata atagtagtca
     4261 aagatggaga actaaaatca tatttttatt cagaaaatta taaaccagtt aagaaaagtt
     4321 gtatatttga acacatatac tttgcaagaa atgatgcaac tatagacaat gtaaatgcat
     4381 atgaatttag aattaaatgt ggtgagagat tagctcagaa tgaaactgta aaggcagata
     4441 tggtagttcc agttccagat tcaggttggc caggagctat tggttatgca aatgctagtg
     4501 gattaaaaat aagtgaaggc ttagttaaaa atagatatgt aggaagaaca tttataaaac
     4561 ctacacaaga agaaagagaa atagcagtaa aaataaaatt aaatcctctt tcaacaataa
     4621 taaaaggaaa atctataatt ttagtagacg actcaattgt aagaggaact acatctaaac
     4681 aattagtaaa atctttaaga gaagcaggtg caaaagaaat tcatcttaga ataacttctc
     4741 ctccagtagc ttactcttgt tactatggaa tagatactcc aaatcgctct aaattaattg
     4801 catcaagtaa caacgtagaa gaaatgagag aatatattgg atgtgatagt ttaaaattcc
     4861 tagatataga aggaatgtta gatgcaacag aacataagtc tactttctgt aaagcttgtt
     4921 ttgatggaga atacccagtt aagaaaattg ataaggagga actactttca tgttaacata
     4981 taaagaatca ggtgtagata tagatgaagg aaatagagca gtagacctta taaaaggtaa
     5041 aataaaagga acttatgatg gaaatgtagt tggagattta ggtaacttca gtggattata
     5101 cagtttaaaa gattttgtag gtatgaaaga accagttttg cttgcatcta cagatggtgt
     5161 tgggactaag ttaaaaatag ctcagatgat ggacaagcat gatactgttg gaatagattt
     5221 agttgcaatg tgcgtgaatg atttaatttg tcaaggagca aaaccattat tcttccttga
     5281 ctatatagct cttgggaaat tagtgcctga gcatatagaa aaaatagttg gtggaattgc
     5341 agatggttgc aaaatgtctg gttgtgcact aattggtgga gaaactgctg aaatgcctgg
     5401 aatgtatgga gaagatgact atgatttagc aggattttct gtgggaatag cagacaaaga
     5461 aaagattgtt tctggaaaca atgttaaatc aggagatgta ttggttggta tatcttcaag
     5521 tggagtacac agcaatggat tttcttttat aagaaagata tttttagaaa cttacaatta
     5581 taaaatggaa cgatatgttg aagaactagg aatgacagtt ggagaagcac ttttaacacc
     5641 aactaaaata tatgttaaat tagctttaga tgtattagct aaacatgaca taaaagctgt
     5701 agctcacata actggtggag ggcttataga aaatataaca agagttatac ctaaaggatt
     5761 aggactagat atcaataaaa aatcttggga gaagcctcct atatttaaaa tgatagaagg
     5821 ttttaatgct gttgatgaga gagaacttca taagagtttt aacatgggta ttgggctagt
     5881 tttaatagta gataaagaaa atgctgatga tgttgtaaac tttataaata atagagaaaa
     5941 tgacaatgct gattatgtag ataaaaaata tagtgaatta ttagaagata aagcttatat
     6001 aattggtgaa gtagtagata gtcatgaagg tgtagaatta tgctaaatat aggagtttta
     6061 atatctggtg ggggaacaaa tttacaagca gttatagatg gtactgagtc tggagaaata
     6121 aaaggtcagg ttaaagtagt tatctcaagt aagcaaggtg cttatgggct agagagagct
     6181 aaaaatcata acataaaagc aatttgtgaa actgatgaag acaagataat agaaattctt
     6241 aaggaaaata aaatagattt agtagttttg gctggttatt taaaaataat cagcccaaag
     6301 ctggttaatg agtttagaaa caaaatgata aacatacatc catcattaat tccttcattt
     6361 tgtggagctg gattctatgg agaaaaagtt catcaaggtg ttatagatta cggagctaaa
     6421 gtaactggtg ctacagttca ttttgttgat gaaggtgctg atactggacc aatcataatg
     6481 caagatgtag ttaaggtaaa tcaagatgat gatgcaaaga cacttgcaaa aagagtgtta
     6541 gaagttgaac ataggatttt aaaagaaagt ataagtcttt tttgtgaaaa taaattaaaa
     6601 cttcaaggta ggagagtgtt tataaatgag taagagagct ttaataagtg taactgacaa
     6661 gactggtgta gtggaatttg ctaaagagtt aaataaatta gattatgaaa ttatatctac
     6721 aggaaataca tttaaaacat taaaagaaaa tggtgttaat gtaatgcaag ttgaagatgt
     6781 tacaaatttt ccagaaatat tagatggaag agtaaaaaca ttaaatccat atatacatgg
     6841 tggtatactt tataaaagag ataaagaatc tcatgtagaa actgtaaatg aacataaaat
     6901 acattcaata gatttagtag ctgtaaatct atatgatttt gaaggaactt taaaagctgg
     6961 aaaaagtcat gatgaaataa ttgaaaatat agatattggt ggaccatcta tgatacgttc
     7021 tgctgccaaa aactataagg atgtaatagt agtagtagat ataaaagatt atgattctat
     7081 aatagaaaaa ttaaaaacag atacaatgac attagaagac agaaaaaaat tatcatataa
     7141 agctttctca actacaggaa gatatgatgc gttaatatct agttattttg ctggagaagt
     7201 tggagatacg tatcctgaca tattaaattt aactttccaa aaagaacaaa ctttaagata
     7261 tggagaaaat ccacatcaaa atggtttctt atattcacag tcaaatgcaa aaaatccaat
     7321 cttaaattat gagcaattag gcggtaaaga actttctttt aacaacttaa atgatttaca
     7381 tggatgttta gaagtaatga gagaatttaa agatagtgaa gaagtagttt cagtagctat
     7441 aaaacatgct aattcatgtg gggtaggttt aggaaaagat gcttttgaag catatacaaa
     7501 atgttatgaa gcggataagg tttcaatatt tggaggaata gtaggaataa cttctactat
     7561 agataaagct acagcagaga aattaaatga aatattctta gaaatagtag tagcatatga
     7621 ttttgaacca gaagctttag agatacttaa acaaaagaaa aatctaagaa tattaaaatt
     7681 agctaaaata gaaaatagtt tacaaccata tgaaatgaaa tatttagatg gaaaattatt
     7741 gatacaagat agaaacaata tattagcaga aaaaagtgaa aatgtaacaa aggaaaaacc
     7801 aacagatgca caacttaagg acatggaatt tggtatgaga gtagtaaaaa atatgaaatc
     7861 aaatgcaatt gcaatagtaa aaaatggaca aactctagct ttaggttgtg gacaaacatc
     7921 aagaatttgg gcattaaaaa atgctcttga aaataacaaa gataaagatt ttacaggagc
     7981 tgttttagcg tcagacgcat ttttcccatt tgatgattgt gtaacattag ctcatgaata
     8041 tggaatatca gcagtagttc aaccaggtgg ctctataaaa gataaagatt caatagaagc
     8101 ttgtgacaag tatgatatgg ttatggtatt tacaggaata agacacttca aacactaagg
     8161 aggatattat gaagatattg gttgtaggcg gtggcggaag agagcatgct atatgctgga
     8221 aactaagcaa agaaaaaaat gtagaaaaaa tttattgtgc accaggtaat gcaggaattg
     8281 caaacgtagc agaatgtgta aatataggtg atactaatat agaagaactt ttaaaatttg
     8341 ccaaagaaaa tgaaataggt ctgacaatag taggaccaga agtaccacta gttatgggaa
     8401 tagtagatga gtttgaaaaa gaaggattaa gagtatttgg acctaataaa aaatgtgctc
     8461 aacttgaagg aagtaaagct ttttcaaaag aatttatgat aaaacataac atacctactg
     8521 caaaatataa agaatataca aacttagaag aagctatatc agaaatagat agttttggat
     8581 atccagtagt aataaaagcg gacggattag ctgctggaaa aggtgttgta atacctgaaa
     8641 atagagaaga tgctatagca actttaaaag aaatgatgtc agataagaag tttggagctg
     8701 ctggagataa aatagtaatt gaagagtttt taaaaggaat agaaacttca attctagcat
     8761 ttgtagataa tgatacaata gttcctatgg caagtgcaaa agaccataaa aaagtaaaca
     8821 actatgagca aggtccaaac actgggggaa tgggaacttt ttcacctagt gaaatatata
     8881 cagaagaatt agcaaataaa gtaaaagaga cagtattaga aaaaacttta gaaggcttca
     8941 aaaaagatgg acttaatttt aaaggtattt tatttgttgg tcttatgata actgaagatg
     9001 gagaaaaagt acttgaatat aatgtaagat ttggagaccc tgagacacaa tctgttttat
     9061 ttagattaga aacagattta catgagataa tggaagctat actagataat aagttaaaag
     9121 atatagaaat aaactatagt gatgatgaag ctgtatgtgt tatgcttaca tcaggagggt
     9181 atccagacag ttatgaaaaa gggaaaatta taactggtct tgaaaacttg gatgatgata
     9241 tagtagtatt ccatagtggc actaagatgt ttgatggtaa tttggttacc aatggaggaa
     9301 gagtaattgg tataactgca aagtcaacta cagttaagga tgcagcagaa aaagtttatg
     9361 aaaacataaa gaagataaac tttgaaggaa tgcactatag aacagacata ggaagataat
     9421 ttttctaaaa tggaggataa ggtatgttga atactgaaaa taaagactca atggttagaa
     9481 gagtattagt tgaaaaaaga gaaggttttg accttgaggc taaagcttta aaaaaagact
     9541 tagtagaaag tttacacata gataatatag aaaatttaag aatattaaat agatatgatg
     9601 tagaaggaat atcagaagaa gtttatgaaa atgcagcaaa gacaatattc tctgaaccaa
     9661 atttagatgt agtatattat gaagaaatac caaaattaaa tgatgaaaga gtatttgcaa
     9721 tagaatttct tcctggtcaa tacgaccaaa gaggtgattg ggcagctcaa tgtgtacaaa
     9781 ttgtaaatca agggataaga cctgctatta atacagcaaa agtttatata ttatcaggca
     9841 aaataactga tgaagagttt tctaaaataa aagattactg tataaatcca gtagatagta
     9901 gagaagcatc tttagaaaaa ccagaaacgt taaaaatgga aactgaaata ccaacaacag
     9961 ttgaagtatt agatggattt atagatttag atgaaaatgg attaaaaaca tttgtaagtg
    10021 aaaaaggatt agcaatgact ttaggagact tacaacacgt tcaaaaatac tttaaagata
    10081 cagaaaagag aaatccaact ataacagaaa taaaagtatt agatacttat tggtctgatc
    10141 attgtagaca tactactttt atgactgaaa tagaaaatgt aaagatagaa gatggtaagt
    10201 ttaatgatat agtaaaagaa gcgtatcaaa tgtatcttaa ctcaagagac aatgtatatg
    10261 tgaacagaca taaagatata tgtttaatgg atatagccac tgttgcagta aaagaattaa
    10321 agaaaaatgg gaaattaaat gacctagatg aaagtgaaga aataaatgca tgtagtataa
    10381 acgtagatgt tgaagttgat ggtaagatgg aaaaatatct agtaatgttt aaaaatgaaa
    10441 ctcacaacca tccaactgaa atagaaccat ttggtggagc agctacttgt ttaggaggag
    10501 ctataagaga cccattatca ggaagaagtt atgtttatca agcaatgcgt gttacaggaa
    10561 gtgctgaccc aagaacgact ttagaagata ctttacctgg aaaacttatg caaagaaaaa
    10621 taactacaga agctgctcat ggatatagtt catatggtaa ccaaataggt cttacaactg
    10681 gacaagttgc agaagtatat gatgaaaact ttgttgcaaa gagaatggaa ataggtgcag
    10741 ttatagcagc agcaccaaaa gagaatgtag taagagaaag acctgaagca ggagatgtaa
    10801 tcgttcttct tggaggaaaa acaggaagag atggctgtgg aggagcaact ggttcatcta
    10861 aagaacatag tgaagagtca atcttaactt gtagtgcaga agttcaaaaa ggagatgctc
    10921 ctaacgaaag aaaaatacaa agattcttta gaaataaaga agttgctcaa atgataaaaa
    10981 gatgtaatga ttttggagca ggtggagttt gtgttgctat aggagagatt gctgatagct
    11041 tagatataaa cttagactta gttcctaaaa aatatgatgg acttgatgga actgagcttg
    11101 caatttcaga gtcacaagag cgtatggcag tagccataaa aaaagaaaat aaagataaat
    11161 ttatacaatt agcagtagaa gaaaacttag aagctacgca tgtagctaca gtaacagata
    11221 caggatattt aagaatgttc tggaatggaa aagcaattgt agatataaat agagagtttt
    11281 tagatacaaa cggagtaaaa caaactactg atgttcatgt tactaaagta gatgaagaaa
    11341 atacattctt tagctctaat gaaatagtaa aagatgttaa gtgtgcaagt atgaaagaca
    11401 agtttacaaa agtactatct gaccttaatg tttgttctca aaaaggatta gtagagatgt
    11461 ttgataatac aattggtggt aatacagtac ttatgccatt tggaggaaag tatcaagcga
    11521 ctccaacaca aggtatggtt gctaagatac cagtacttgg aggagaaact aatacttcta
    11581 caataatgac ttatggatac aatccaaagg taggaaaatg gagcccattc catggtgcct
    11641 tgtatgcagt agtagaatct gtgtgtaaat tggttgctat tggaggaaat tatagtacaa
    11701 caagacttac attccaagag tattttgaaa aacttggtaa caatccagaa aaatggggta
    11761 aaccattctc agcactttta ggagcatttt atgcacaaag taaatttgag atacctgcta
    11821 taggtggaaa agacagtatg tctggtacat ttaaagatat tgaagttcca ccaacattag
    11881 tttcatttgc tgttgacaca gtagatgcga agaaagtagt atctccagaa tttaaaaaag
    11941 cagactcaaa agtagtaatg ttatgtgtaa acaaggcaga aaatgatgtt gtagattttg
    12001 aggaattaaa gagaaactta gataaagtaa gagaacttat acatggaaat aaagttttat
    12061 caacatatgc attaggattt gcaggtgtag gtgaagcaat aagtaaaatg gcttttggta
    12121 ataaaatagg atttaagttt agtgaagagg ctgaaaaagc atttacagat gataaattat
    12181 ttgaggctag ttatggaaat atagtacttg aattagcaaa tgatgattta agtatgttag
    12241 aaggatacaa ttatgttgtg cttggaagta cagttaaaga aactagtata tttatcaaag
    12301 gtgaagaact tgcattagat gaattatata aagcacattg tagtacttta gagcctatat
    12361 tcccaactaa aacagaagaa gtaaaatcaa agatagaaac tataagttat atatcacaag
    12421 gagaagctaa aaaatcatca ttaagtatag ctactccaag agtatttata ccagcattcc
    12481 ctggaacaaa ctgtgaatat gactctgcaa gagcatttga aagagcagga gctaatgcaa
    12541 gtataagagt atttaagaat ttaacttata aagacataga agattctata gatacaatag
    12601 taaacgaaat aaaatcttct caaataataa tgttaccagg tggatttagt gctggtgatg
    12661 agccagatgg ttctggtaaa tttatagcta ctgtatttag aaatcctaga gtgcaagaag
    12721 ctataaatga atttttaact caaaaagatg gactaatgtt aggtatctgt aatggattcc
    12781 aagtattaat taaattaggg ttagttccat atggagaaat aagagttcca agtgaaagtg
    12841 ctccaacact tacttacaat aatattggtc gccatcaagc taagattgct agaactagaa
    12901 tttcatctaa taaatctcct tggttagctc aaactaatgt tggagatatt cataatatag
    12961 caatttctca tggagaaggt aagtttgttg ccagtgaaga tgttatgaga gaattaattg
    13021 ctaatggaca agtagcaact caatatgttg actttaataa tgaagctact tatgatattg
    13081 agtttaatcc aaatggttca ttctatgctg ttgaaggtat aactagtgct gatggtagag
    13141 tgtttggtaa aatgggacat tctgaaagaa ttggtgagga agtatataag aatattatag
    13201 gtgaaaaaga ccaaaagata tttgaatctg gagttaaata ttttagataa agctttgatg
    13261 tttaattgat agctagaata tataatttag atatttagat ttaaagagat gtaataatat
    13321 gatgataatc atttaatatt acatctcttt tttatttatc taagaaataa taagttcaac
    13381 ctaagttaaa atacaagctg tgttataatt aattattaag taaaattgaa tatttattgg
    13441 aggacaacat aattaatgaa aatattaata acagggtcta atggtcaatt aggaaaagaa
    13501 ttggttaacc aattagaagc aatcaatcaa agtataaacc aaccaaaata tgttatatta
    13561 gctacaacaa gaagtgattt ggacatatcc aatcagacta atgtagataa ttttattttg
    13621 cataataaac ctgatgtagt tgtaaactgt gcagcatata caaaggttga tgcatgtgaa
    13681 gataatatcg aaattgcata caaaataaat gctttaggag tgagaaatct agcaatagca
    13741 tcagaaaaag ttaatgctaa gcttattcac atatcaactg actatgtatt caatggtttt
    13801 tctaaatatc catataggga agacaataaa actgaaccca attctgtata tggaaaaagt
    13861 aaacttatgg gagaaaaatt tgttgaacaa ttctcacata agtactttat attgagaaca
    13921 gcatggttat atggtaatgg gaataatttt gtaaagacca tgataaaact ttctttagaa
    13981 aataaagagg ttaatgtggt taatgaccag tttggaactc caacaagtac agtagactta
    14041 gcaaaaatta taataaaaat catggaaaca gaacattatg gagtttatca tgctacatgt
    14101 gaaggtgagt gtagttggta tgattttgcc aaaaagatat ttaaactaaa aaatatagat
    14161 ataaaagtaa atccaattaa gagtaacgaa tttaaatgta aggctccaag accactatat
    14221 tctgtattgg ataattttat gttgaagtta attggtttaa attcttttag aaagtgggaa
    14281 gaatccattg aagaatattt agaaagatat gatataaatg aaaagtaaat gtattatttt
    14341 gagaaaagaa aataagatat tataaaaaat ataaatattt cttttattta tccgataatc
    14401 ataatatgac aaaaacaaat ttaaggatgg aaaaagatga aaggaattat tttagcagga
    14461 gggtctggta caagactata tcctataaca aaatgtatat caaaacaaac acttccaata
    14521 tacgataaac caatgatata ttaccctatg tcggttctta tgttggcagg gatacaagat
    14581 atattaataa tatctacacc aagagatata aaagcatttg aagaattatt tggagatgga
    14641 agttcactag ggcttaacat aacatatgca attcaggaga aagcaaatgg aattgcagaa
    14701 gcgtttataa taggagaaga atttatagga aatgacagag tgtctcttgt attaggggat
    14761 aatatatttt atggatatgg atttagtgaa aggttggaga atgcagttaa aagaaaagga
    14821 gcaactatat ttgggtatca tgtaagtaat ccaaattcgt ttggagttgt agaatttgat
    14881 aaggatttta atgtattgtc aatagaagaa aaacctacag ttcctaaatc taattatgct
    14941 ataccaggat tatattttta tgataatgat gttgtagaga tagcaaagct agttgaaccg
    15001 tcagatagag gtgaattaga aataacctct ataaacagtg aatatctaaa acgaggaaaa
    15061 ctaaaagtgg aacttctagg aagaggtatg gcttggcttg atactggtac acataaaggt
    15121 ctattggatg cttctaatta tgttgaagtg gttcaaacaa gacagggact gtatatagca
    15181 tgtttagaag agattgcata tacaaaagga tatataagta gagaacaatt aatgaaacta
    15241 gccaagccac tgataaaaac tgaatatggt caatatctat taaaattggc tagtgaagat
    15301 atgtaatatg gtagataaaa tttattataa attttattaa gggagtttat tataatggga
    15361 aaaatcaatg ttataaagac taaaatagaa ggattatata tagtcgaacc aaatgtattt
    15421 tatgatgaaa gaggatattt tatggaaagc tataataaaa aggattttat agagcagggt
    15481 cttgatatgt gttttattca agataatgag tctagctcaa gaaaaggcgt tttaagagga
    15541 atgcattttc aggttaaata tcctcaagga aaattagtca gagttacaga gggagaagtt
    15601 ttcgatgttg cagttgattt aaggaaagat tcacaaacat ttgggaaatg ggaaggtgta
    15661 atattgtctg ataagaataa aaaacaattt tatattccag aagggtttgc acatggattt
    15721 ttagttttat cagaaaaagc agtatttagt tacaaatgta caaattatta ttacccagaa
    15781 tttgagaatg gctttatatg gaatgacaag caaatatcta ttgagtggcc tttaaaagga
    15841 attgaagaaa tatttttatc agaaaaggac aaaaaacaaa aaagttttaa agatatttgt
    15901 acaagacgtt aataaaaatt tattagaatt aacatttagg agaaaaaata tgaaaaaaat
    15961 actgataact ggaggagcag gatttattgg gagtaatttt atacattata tgcttaacaa
    16021 gtatgtagat tattttattg ttaacctaga cttgttgact tatgctggta atttagagac
    16081 attaatagaa atacaagaca attccaatta taaatttgtt aaaggagata tttcaaatag
    16141 ggagcaggtg tataagctat ttgaagaaga aaggtttgat atagttgtta actttgctgc
    16201 tgaatctcat gtagataggt cagttgagaa tcctgattta tttattaaaa caaacattat
    16261 aggaacacaa gtacttttag atgcatctat aaaatatgga gttaaaaggt atcatcaaat
    16321 atctacagac gaagtgtatg gtgatttacc aattgataga aaggatttat tctttacaga
    16381 acaatcttct atcaatccaa gttctccata ttcagcttct aaagcatcag cagatttatt
    16441 ggtaagttct tattatagaa catatggatt gttaacaact atatctaggt gttccaacaa
    16501 ttatgggcca tatcattttc ctgaaaaatt gattcctctt atgattttaa atgctttgga
    16561 aaacaaacaa ttgccagtat atgggaatgg ggaaaatgta agagattggt tacatgtata
    16621 tgaccattgt actgctatag acttaataat acataaagga aatatagggg agatttacaa
    16681 tataggaggt cataatgaaa gaagtaatct agaagtagta aaaatgatat taaatctttt
    16741 aggtaaatct gaagagttaa tatcctatgt aaatgataga cctggtcatg atttaagata
    16801 tgctattgat gcaactaaaa tagagaatga gttaggttgg aaggcaaaat atgactttga
    16861 tttgggtata aaagaaactg ttaagtggta tatagaaaat gaatcttggt ggaaagctgt
    16921 tttatcagta taactttaaa atttagaaag gggcgtttta tattgaatga gataagttca
    16981 ctaattaata tattagcatt gactcaatta tctggtagta atactacaaa tcaatgtaat
    17041 tgtacatata ataattcatc tggatttgat atgattatga tgaccttgtt aaaagcatta
    17101 tcacaaaata atcaacatac ttctaatagc tacaatttat tgtctaattc tgagaattta
    17161 tttaatgaac ttgataatgt agtaaataat accactagta gatttataga tgttgataca
    17221 aaagataaaa atgtaaagtt gagaattgaa aatgctgtaa atcaagcatc aaaaaaatat
    17281 aacgtagatt caaatcttat aaaagcaata attaaagttg aatcagattt taatccaaat
    17341 accgtttctt ctgctggtgc taagggtctt atgcaactta tgccagaaaa ctgcagagac
    17401 ttaggggtta cagacccttt taatatagaa cagaatatag atgctggaac tagacatatt
    17461 aaggaataca ttgatatgtt tggagggagc atagaaatgg gattgatggc atataatggt
    17521 ggtcctggaa gaatgaaaag tagaggagta gaatcaatct ctgacctgta taaaatgcca
    17581 aaagaaacac aaaattatat tcctaaggtt atgaaatatt atagagggta ggctttatta
    17641 tgggaataaa tatagataag ctaaacataa gtccaagtat aaatatggag gttaaaaaac
    17701 taaaagagag taataaattt tctgacaaat taaactcttt aaaacaatct tcttcattgg
    17761 aagatataaa acgaaatttg gaaagtgtaa aaaaggcagg agaaaggctt gtacttactc
    17821 aaaactatgg cgatattagt atatataaaa atgcagtaaa agaataccta aaatctgttg
    17881 tagataatat gtattcactt gataaaaata gtagcttttg ggagcatcag tattataaga
    17941 atgtaaagat tgttgatagt aaacttgagg atatgactag taagttaatt agtgaagaaa
    18001 aagaaaacat agatattgtt tctactgttg acatgataca aggcttattg atagatatgt
    18061 atagataagg ttatacaagg agaaaaattt tatgaatagc aaaaaagagg atatatatga
    18121 attgaatttt gggcaattaa tagaagaaaa atctggtgtt atagaagaaa atcaaaatgt
    18181 ttttaatgca aaagtaaagg tagctgtttc tataggaaat acaacagaaa caataaaaaa
    18241 tatattagat ttaaaagaag gctctataat aaaattaagt aagaatgtgg atgaaaaatt
    18301 agatatatat gcaaacgata gattgtttgc ttatggagaa agcattatta caaatgagaa
    18361 gttatctgta agattatcta aaatagaaga ttcagatgta aattagcatt ttaaatatat
    18421 ccctttagtc tattctgtat gagtttagct aaggggatat ttttataaaa aatttaaaat
    18481 atgtgtgaat tatataaata tttttatttt atgagcgata actaaattat aagtatgtaa
    18541 ttttggaggt gaaactatgg atattaaaaa tgtaagttca aatattgtag tgaataatta
    18601 tttaaagaat acatctggta taaaaaaagt ggaagaggtt aaaaaaatta ataaaacaga
    18661 caagatagag ttatctaaat ttgcaaagga aatggcaagt gtgtcaaata tgagtgatga
    18721 gaatattaga aataaaaaag tcgatgaaat aaaaaatgca ttagaaaatg gaacttacaa
    18781 aataaaacca aaagaaattg cgaaaaaaat gatagaggat atgcgaggat aaagtcatga
    18841 ttacagactt aaaagttata atatatgaag aaaaaaatat aattagtaag atgatggaat
    18901 tattaaaaga acaatttgat tatatagttg aaaaagactt agaaaattta aatatgttaa
    18961 atcctaaact agaagatatt agcagggagc ttgcatccat agaaataaag agaagacaac
    19021 tttttggtaa tgatatttca atgagtgaag ttgttgaaaa tagtaatgat ggatatttaa
    19081 aagaaatata tacagacttg aaacgcattc ttgctttagc taaaaatcaa caagaatcaa
    19141 atgattctct aataaagaaa gaacttattt ttacgaaaaa aatgattaat ttcataaaac
    19201 cagctgataa gcaaactaca acttataatt cttatggaaa tattagaaaa taaatattag
    19261 gaggagaaat atggcaggat tattttccat tttaaataca gctaattcag gaatgaatgc
    19321 gcaccaaaaa gcaatacaga caatttctca taatataagt aatctagata cagacggtta
    19381 ttctaggcaa aggaccgagt ttgctacaaa tagtccaatg tatatgccta gtttgagtaa
    19441 ttcaattgca agaggacaat tagggactgg agtacatgta acggatgtaa caagagctag
    19501 aaatagtttt tatgattacc aatttagggc agaagctcat aagtatggta agacagtttc
    19561 aaaatatgat tattacaata ctatagaaac tatactaaac gaaccatcag actatggaat
    19621 atctgcaggt atagatgatt tttttaaagg atgggagtca ttatcaaagg atataaatag
    19681 tgacagtaaa aaaagtctag tggtagaaaa cgcatctaat ttagcaactt taataagtga
    19741 aagttataag aagcttgata agttaaaaga agatatagat tctaatatag acactgagat
    19801 aaaacaaata aatgacatgt taaaaagtct tgaagattta aataaatcta ttgatattat
    19861 atctggttct ggctctacac caaatgattt gcttgatgaa agagatagaa ttttagataa
    19921 cttgagtttt aaactagact ttgaaaatag tgatgttaaa aatatgcttg cagatggtaa
    19981 attagaactt aatgaattaa aaaatccaga tggaacttgg aaaacaggta tatcagggac
    20041 cttacaaggc ttgtttgaaa tgcatggtaa gattgataca tataaaagcg atttaaaaga
    20101 tgtatctgat ggattagcta aacagataaa tgatgtttat aatacgtctg caggaataac
    20161 agtaacagat ttttttataa cttcaaatgt agatggtgaa gatattataa agataaatcc
    20221 agctataaag agtaacccaa atgaattaaa gcttactgct gatgaagctt caaaaattgc
    20281 taagttaaag gatgaaaaaa tagatattgg ggtggctggt ggaaaagtaa gtacaataag
    20341 tgaccactat aaagcctttg cagaatcaat cggtttagat tctcaaaaag taaatcaaga
    20401 tgaagcaaat caacgtaaaa ttataaataa tattgataac tcaagaatga gtgtttcagg
    20461 tgtatctttg gatgaagaaa tgacagagct tataaaaata caacgttcat atcaagctag
    20521 tgcaaaagtt atgtcaacag ctgtgcaact actggatgtt gtaataaatg gaataattta
    20581 atagaagggg gaatttataa tgagagtcag tacaggtatg atgtctagca gctatctaaa
    20641 ctcactacaa gataatctac aaagattgga taaggttaat agacaagtta atacaacaaa
    20701 agaaataaac aagctatcag ataatccata taaagcaata aagatattaa actcaaaaag
    20761 tgaaataaag actatggaga cttatgttga gaattgtaaa gatacaggga attggttaga
    20821 aactactgat actgcattag atcagcttgg aaaattatta tcagacataa agaaaacttt
    20881 ggtggcaagt ggtgatggtg catactcaga tgatgaatta aagactctta gtaatgaaac
    20941 taatgaaaaa atgaaagaaa ttgcaaatgc tttaaatgca acacatgaag ggaagtacat
    21001 attttcaggt tcaaatacgg gggtaccacc tgttgaatgt atagataatg ctgatggttc
    21061 agtgagttta aagtttaatt ctagtctaaa tcttaataaa ttaaatgact ctttgagtat
    21121 ggatatagct caaggaataa gtgtagatta taacgttaaa ttgagtagcc ttggatttga
    21181 cccaacaaaa actgacccat ttgaagagtt aaataacata tcaaaaaaat tagcaaatcc
    21241 agatgctgat gctataaaag agcttacaac aacttgtttg ggcgatatgg aacaactaat
    21301 agaaaataca gtaaatgtta ggtctatata tgggacaaaa gcaaatacag tagaggctat
    21361 gaaggaaaaa aacgatgata gtttgataca gttaaaggat attttgtcac aaaatgaaga
    21421 aatagattat ggtgaaaaac ttgttcaatt aaaggcagca gagttaactt atcaagcttc
    21481 actacaaaca ggaggaaagt tatttaatgt atctatatta gattacatat aataaaataa
    21541 cgcagatatg atgaaggtta cattaaaaaa aggtatatta ggttttgaaa atttaaaaga
    21601 atatgagctt ttagatatag aagataatga ttatttaaaa gaatttaatt ctactgaaga
    21661 agaaggtata ggatttgttg ttgtatctcc atttgagatt atcaatgaat atgaaatagt
    21721 tttaaatcag gaaacaatag aaaaactaga agtgaaatct ccaaaggatg ttgtattatt
    21781 taatataatt actcttggtc agacatttga agaatcaact gtgaacatga aagctcctat
    21841 agtgataaat ataaaaacta attttggaat gcaaataatt ttacaggatg ataaatattc
    21901 aataaggcat ccattattga gaggtgataa tggatgctag taatttcaag aaaaaaagat
    21961 gaagcagtac ttataggaga aagcatagaa gtaaaaattg taggagtaga tggaaacaat
    22021 gtaaaactag ctatatcagc gcctaacaat ataagtatat taagaaaaga aatatacgaa
    22081 aaagtaaaga gtgagaatat aaaagctaca aataaaaata taaaaatttt aaagtcatta
    22141 aaataataag cttggaggaa gtaatatatg tatggagaaa atccttataa ttcttataaa
    22201 caaaatgctg tttttatggc atcaaaggaa caattgttac ttatgcttgt tgatggagca
    22261 gtaaaatata ccaaaatagc cagagcagct atcttagata aaaatacaca gaaagcgcat
    22321 agagaacttg taagagttca ggatatattt acagaactaa tggttacatt agaccaaaat
    22381 gcaggacaat gggcaaagga tatgtacaaa gtttatgatt ttgtaagata tgaactttca
    22441 agagccaata ttagaaaaga catccaggta atagataatg tgttacctgt aatagaacaa
    22501 attaaagata catggcatga agctgataaa aaaagtaaag aagaaagaag taggtacaaa
    22561 taatggaatt aaatttagct gaattagata agttatcaaa agaggaatta ctattgatgc
    22621 ttgtagatgg cacagtaaaa tataccaata tatccaaaga agctctttta aataacgact
    22681 atttgaaagc acataatgaa cttgtaagag ttcaaaatat atttacagaa ctaatgacta
    22741 cattagacca aaatgcagga caatgggcaa aggatatgta taaagtttat gaatttataa
    22801 aatctgaatt agcaatagct gatgaaaata aagatattaa aataattgat gatatattgc
    22861 caatagtaaa acaaattaga gatacatggc atgaagttta caatcaacta aaaatataat
    22921 tagatggagg gataaaggta tgtcaagtat aagtcctata agagttacag gtctttcagg
    22981 aaattttgat atggaaggca taatcgaagc tagtatgatt agagacaagg aaaaagttga
    23041 taaagcaaaa caagaacaac aaatcgttaa atggaagcaa gaaatatata gaaatgttat
    23101 acaagaatca aaagatcttt atgataaata tctaagcata aattctccta atagtatagt
    23161 aagtgaaaaa gcatactctt ctacaagaat aaccagttct gatgaaagta ttatagtagc
    23221 aaaaggttca gctggtgcag aaaaaataaa ttatcaattt gcagtttctc aaatggctga
    23281 accagcaaaa tttactatta aattaaattc aagtgaacct attgttcgac agttccctcc
    23341 aaatgccagt ggagcgagtt ctttaactat aggagatgta aatataccaa tatctgaaca
    23401 agatactaca agtactattg taagtaagat aaactccctt tgcgcagata atgatataaa
    23461 ggcttcttat agtgagatga caggtgaatt gattatttca agaaaacaga ctggttcgtc
    23521 atcagacatt aatttaagag taattggaaa tgacaattta gctcagcaaa ttgctaatga
    23581 taatggtatc acatttataa atgatgctgg tggaaacaaa gtggcaaatg tatatggaaa
    23641 gaatctagaa gctgatgtaa ctgatgaaca tggaagagta actcatataa gtaaagaaca
    23701 aaattcattt aatatagata atattgacta taatgtaaat tcaaaaggaa ctgcaaagtt
    23761 gacttctgtc actgatactg aagaagctgt taaaaatatg caagcatttg tggatgatta
    23821 taataaactg atggacaagg tctatggttt agttactact aaaaaaccaa aagattatcc
    23881 gcctcttaca gatgcccaaa aagaagatat gacaactgaa gaaatagaaa aatgggaaaa
    23941 gaaagctaaa gaaggtatac ttagaaatga tgatgagtta agaggttttg ttgaagatat
    24001 tcaatctgca ttttttggag atggaaaaaa tattattgca ttaagaaaac taggtatcaa
    24061 tgaaagtgaa aattacaata aaaaaggtca aatatcattt aatgcagata ctttttcaaa
    24121 ggctcttata gatgatagtg ataaggtata caaaacacta gcaggttatt cttcgaatta
    24181 tgatgataag ggaatgtttg aaaagctaaa agatattgta tatgaatatt ctggaagttc
    24241 aacttctaaa cttcctaaaa aagcaggtat agaaaaaact gcttctgcta gtgaaaatgt
    24301 atattcaaaa caaattgcag agcaagaaag aaatataagc aggttagttg aaaaaatgaa
    24361 tgataaagag aaaagacttt atgctaaata ttcagcctta gaatctttgt tgaatcagta
    24421 ttcttcccaa atgaattatt tctcacaagc acagggtaat taattatgga taaattaaaa
    24481 gaaaaattaa atttatataa agatatatct ttacaaatta taaatttgat tgaaaaggaa
    24541 gaatatataa agataagtat taagttaggt gaaagacaag agattataaa tagtgtaagt
    24601 gaaattgata gaaatgattt tatacagctt tataatagaa tggaattaat tgaaattgat
    24661 agcaggataa gagatatttt acaaggacaa ttattagaag taaaaaaaga attacatgag
    24721 tataagctta ctaaacaagt gaacacaatg tattataact taaatagaga aaaagtaaat
    24781 atttttaata aaaaagtata aagttatgta ccatcttgtc cgattatata aatgaagaat
    24841 taataaaaaa gattaaaagt agaaatgaca aggatgtcaa ctatactaag gagggtaaaa
    24901 taatgagagt taatacaaat gtaagtgctt tgatagcaaa taaccaaatg ggaagaaacg
    24961 taaatgcaca aagtaagtct atggagaagc tatcttctgg tgttagaatt aagagagctg
    25021 ctgacgatgc tgctgggctt gctatatctg agaaaatgag agctcaaata aaaggtttag
    25081 accaagcagg aagaaacgtt caagatggta tatctgttgt acaaacagca gaaggtgcat
    25141 tagaagaaac tggtaacata ctacaaagaa tgagaacttt atcagtacaa tcttcaaatg
    25201 aaactaatac tgctgaagaa agacaaaaga tagctgatga gttactacaa ttaaaagatg
    25261 aagttgaaag aatatcaagc tcaattgagt ttaatggtaa gaaattactt gatggaagtt
    25321 ctacagaaat aagattacaa gttggagcaa actttggaac aaatgttgca ggaacatcta
    25381 acaacaacaa tgaaataaaa gttgctctag taaatacttc aagtataatg agtaaagcag
    25441 gtataacaag ttctacaata gcaagcttaa atgcagatgg aacttctgga acaaatgcag
    25501 ctaaacaaat ggtatctagc ttagatgtag ctcttaaaga attaaataca tcaagagcaa
    25561 aattaggggc acaacaaaat agactagaat caactcaaaa caacttaaat aatactatag
    25621 aaaatgttac agcagctgaa tcaagaataa gagatacaga tgttgcttca gaaatggtta
    25681 acttatctaa aatgaatata ttagttcaag catcacagtc aatgcttgct caagctaatc
    25741 aacaaccaca aggagtttta caattattag gataaaataa ttttgtatat tggatataga
    25801 agaaagggta aggctttgct ttactccttt ctttttttac aaatatactt ttgaaaggaa
    25861 aatttatatg ttacttagta tagttatgat tgttaaaaat gaagaaaaaa tacttgaaaa
    25921 aactttaaaa tcattaacca cattaagaaa ttctatagaa agtgaactta taatagtcga
    25981 tacaggttct acagatgata ctataaaaat atctaagaag tacacggaaa aagtgtattt
    26041 tcataattgg aataatgatt tttctagtat gagaaatata tcaatatcct atgctaaagg
    26101 tgaatggtta cttatattag atgcagatga aatactaatt gattcttcaa ctattgtaaa
    26161 attttttaaa gatggtcttg ataaaaaatt taattctgct tcaatacaat taaaaaatct
    26221 ttactcatat gataaaaaat tatatggata ttgttcagtg cttagacttt ttaaaaacgt
    26281 tgattttaga taccatggta aagtacatga acaaccaata tacaaaaacc ctatatttaa
    26341 taatgttgct gattttgaac attatggata tttgtttgaa gatgaagaaa taagattaaa
    26401 taaggtgaaa agaaatgaaa aactattatt tcaagagtta aaagagaatg ataattctcc
    26461 atatacaaac tatcaattag ggaaaaattt tattatatta ggaaaatatc aggaggcttt
    26521 atactatctt gagaaatctt atgaattata ttctaaacta gaattggcac ctggttactt
    26581 gataacaagc cttgtaaaaa catatttata tttaggaaaa cataagaagt gtgaaagatt
    26641 gtgcttaaag tatattaaga aagacatgaa taatattgat atatattatt atattgcaca
    26701 agcacaagta gatttaggaa agtatgaaaa tagtatagat tcgtataaga gatatattta
    26761 cttgttagat aattatgaaa taagtacaca agcaaatagt ttattttcag acactgatac
    26821 tgtagggctt agagatgagg ctattattac actgataaag atttattata agcttgaaaa
    26881 atatgagttt gtaatttcag agtataataa cattgaagat gtggaaaaga aaaagaatgt
    26941 gtactttagc ttatttatgt cactatataa attgaataga tttgaagata taaaaaatta
    27001 ttataaagaa gtttcaaatt ctaaaattga aaaaaatagt ttttatagaa atatagaaga
    27061 agttataaat aatattaaag aggatgaaaa agagtatgta tataaaatcc tttgtgacat
    27121 tgaaggtaat tatggcattt taaatcaaat aaggataaat aaatgtgtat ctatagataa
    27181 atgtaaagaa atactcaatg aagaaaagga aataatttat gcatatatta taaaaatagc
    27241 ttttgaagag aagattgatt tactagatat tttttatgaa atggaatatg tatggataga
    27301 gaaatatcta aaatatcttt tagcattaga aagaagtttt aatttagtac tatataagta
    27361 cataattagt cgtccaaata catcagatat ggaaaagata agagtatata aagctatatc
    27421 taaagtagtt ttagaaagta taagcttaag tgatgaaaaa tataaggaga ttttttatct
    27481 ttatataatg tattcttata agtacataag atacatatat agcaatttaa gtgattttga
    27541 attaataaaa tttgtttgta atgatttaga taagttcact atagaattta agcatctact
    27601 tgatattaaa aataaagtaa ataatgctaa tgaaaaatta gagtatatac atagtatgag
    27661 gaaattacta aatgagtatc ctttttataa taaaataata aaaatactta taggtgaatt
    27721 agaagaaaat ataaaagaaa gtgaagaatt taaggattta aaagatagtt ttattattag
    27781 tatagaaaat atgattgaaa ctggcaaaat aaatgatgcg aaattattgg tagatgacta
    27841 ttcaaaaaca tttgtggatg atataaagat tttaaatata aagggaattt tatacatgtt
    27901 tgaaaataag cttgataaag ctgattttat gttaaaaaag gcattatcat tagatgtaga
    27961 aaatgaagat actatatata atatagaata cttaaaaagt ttaagataag tcaaaaatag
    28021 gagaattgaa gaatgaatat accaatagtg gttaatgaaa aatctaaaga cataataaaa
    28081 gattggcaat atacatgtac aaatgaagat ataaaattta caagcataat aatattgaca
    28141 tataatcaaa tagaatatac taaattatgt attgaaagta ttaggaaatt taccccaaag
    28201 aataaatatg aaataataat aattgataat aactcttctg atggaacagt tgagtggtta
    28261 aagggacaat ctgatataaa atctgtgtat aataacaaaa acttaggatt tccaaaagga
    28321 tgcaatcaag gtattgagtt agctactggt gaaaacatat tgttgttaaa taatgatgta
    28381 atagttactc caaattggct ctataatttg aataaagctt tatggtctca agataatata
    28441 ggtgctgtag gacctattac gaatgcatgt tcatattatc agcaaattga tgttgattat
    28501 aagtcaattg aggacatgtt tatatttgca cacaaaataa ataaatctaa tattaaccta
    28561 tggaaatata gattgaagtt agttggattt tgcatgttaa taaaaaaaac tgttctggat
    28621 aatgtagggg ttttagatga attattcaca cctggaaatt ttgaagatga tgatttatct
    28681 tttaggatga tagagtcagg atataagtta ttattatgcc aggatacttt tatacaccat
    28741 tttggaagtg tgtcttttaa agaaaatcgt aataaatata cagatataat gtatattaat
    28801 agaagaaaat ttgaaactaa gtggggattt aatccagaat atagtggaat gataagaaca
    28861 gatttgattt ctaaaataga tgatagtttg gaggcaaata ttaatgtttt agaaatagga
    28921 tgcggtatag gggctactct tttagaaata aaaaatatat ataaaaatgc atcaatatat
    28981 ggaatagaaa ttagtgagtc agcaggtaaa cttgcaaagt atagtgcaga tgtacttata
    29041 gcaaatatag aaaaagtaaa attggattat aaaaaagatt tttttgatta tataatattg
    29101 ggagatgtac ttgaacattt gattgaccca tggaagatag tcaatgaatt gaaacaatat
    29161 ctaaagaagg atggttatct ggtatctagt ataccaaata ttatgcatgt ttctgtttta
    29221 agaaatcttg tgttaggtaa ttttacatat gaagattcag gaattttgga caagactcat
    29281 ttaaggtttt ttacattaaa tgagataaca aaaatgttta atgatagtaa ttacataatt
    29341 aatagtatta cagctactag tatgccactt tcggaaattg ataatagctt cattgataca
    29401 atatgtaagg aaagctgtaa agaaatgaga caacagtacg aatcttatca atatatagta
    29461 aaagcctcta aaatcctaga tactaaaaga tattctagta ttagtatgat taatttaaaa
    29521 tacgctttaa tgagaattga taatgaatta gatgtagata gtaatttaag ttatatattt
    29581 gataattata atataactga accatgtttt gttgatgata ttgaatatct aattgataca
    29641 tttgtgataa ataaagcaac tctattaaat agattaggag ttgaggcatt taacagaggt
    29701 ctaggagatt ttagtataaa catgtttatg acagtattag agatggatag agatgatatt
    29761 gactcaatat ataatataac aagtgtatta tgtgaattag aagaatttga tactgcttat
    29821 aatttaatta aaaatagtag cgaaaaagta cagagtgaaa gtgatataca agagatactt
    29881 tgttttattg aggggaagaa atatgaataa tgataagata tgttttataa cctgtgtaaa
    29941 tgatgaaagg ctttatgaag aatgtcttgt atatataaat agcctagaca ttcctaaaaa
    30001 ctttagtata gaaacagtag ctttaagagg agccgattct attgcctctg cttataataa
    30061 agctatgaga gaaacagatt ctaagtataa ggtatactta catcaagata cttttataat
    30121 taataaaaat ttcatacatg atattttaga gatatttaaa aaaaatacag atgtcggaat
    30181 gattggtgta gctggagtaa aagatatgcc tgtgaatgga atttggtggg attctgataa
    30241 aaaagcaggg aaagtatatg atagtcatac aggtaaaatg gatatttatg aatttagtaa
    30301 ctttgaagaa tgttttaatg aagttatggc tatagatgga ttaattatga ttactcaata
    30361 tgatgtattt tggagagagg ataagtttga tgggtggcat ttttatgatt tgtcacagag
    30421 tatggagttt agacaaaata aatacaaagt tattgtacca aaacaaaata agccttggtg
    30481 tatgcatgat tctggagtag taaatataaa taatggatat gatgaatata ggataaaatt
    30541 tgtaaaagag tacataaatc ttgatttata tagagaaaat ttttatgaat ttgataagaa
    30601 gaaagcaagt accatactta agagtaataa gcatgtaatg gattgtatga aagaggagct
    30661 tacagaacaa ttaagattaa agaattacaa ggatgtttta ggtattgttt ctaaaattgc
    30721 tcatttttct gtttttaatc atactggttt gtattcttgt gaagaatgtg agagagcatt
    30781 gctagaatgt gcaaataatt taccagaaat agaaaatacg aaattattta caaaaaaaga
    30841 tttaaaaaga agaaaagtat tacatgtttt aagtgaagga tattcaacag gaggtcatac
    30901 aagactagtt aaaaattgga ttgaaattga caaagactct gttcattcag ttgtaactac
    30961 atggcaatca gatacactgc cagactggtt attaaaagca ataaatgata gtggtggcga
    31021 agttatatct ttggatttaa tttcagatga ttatttaaaa agagctgtag ctttgaggaa
    31081 aatagcttat gactgggctg atatagttat tttatatgtt catatgtggg atacaatacc
    31141 tgttgttgct tttggagttg atgggaaaat acctatttta catgttaatc actatgatag
    31201 tactttttgg ttagggacaa gtattgcaga tattgtcatt gattttagcg aagctagtca
    31261 attaattaca ttagataaaa gaggcataga taaatctgaa atattaccaa tgccattaaa
    31321 agaaaaagaa gtattttcaa aatctgaagt tagaaaacga tataatttag aggatgatgt
    31381 gacaataata ttgactattg ctagtgaaag aaaatacaat agtgtaaatg atattaatta
    31441 ctttaaaatt gctaaaacat tgattgaaaa tacagataat acaattatta ttgttgtagg
    31501 agttgataca gagaatctct attggaaaac tttaagtaac gttacagata atagagttat
    31561 atgttttgga acaacagcaa atatagacga tttttacaag atttcggata tatatatgga
    31621 gtctttcatg gtaggaagtc atacttcaaa actagatgca attttatatg atttaattcc
    31681 tgttaagttt aaaaatgaag tttcaccaat ttttacaaat atgtggaatt ttatagataa
    31741 gtttgattat aagaatataa aagaggttat agatatgata aaaaggtaca atactagtga
    31801 atttgacagg gataggataa aagaaaaaca atcggaaata aaacattttg ttatagataa
    31861 acactgtatt gaaataaaaa actatataga caaaatatat aatgaagcta atcatcataa
    31921 agttaagtat gatttatata aaaatgatga aatcgaggac tatcagcttt ttgtagcatt
    31981 atatggctat atggtaaaaa ataaaaacat gtcataggtg gtaaaatgaa caatattcat
    32041 atcaataaat ttgaaaaaag ctgtgaaaga aactgggatt attttgtaga aaataaatct
    32101 ataaatggta catttctgca aactagaaat ttccttaatt atcatgagga gggaaaattc
    32161 actgaccact cattaataat atgtaaaggt gaagaaatac tagcagtcgt acctgcatgt
    32221 gaaacttatg aagatggtga aagaatattt ttttctcatt tagggagtac ttttggtggt
    32281 attataataa gaagtggttt taatgatatt aggcatgtaa atgccattac aaataagtta
    32341 gaaatatatt tacgggaaca aaatttcgat aaagtatatt taaaaaatac atcaagaata
    32401 ttttgtaaag aggatacttc attactagaa tattttttat ttaagaataa ttacatgttt
    32461 tatgatgaat taagtatgtt tttagaatta agtaagtata aagaagatat attagccaat
    32521 tttaaataca gtaaaagaag agattttaaa tattctttga agaataatct tacctttaaa
    32581 agactgtata gtgatgaaga tatagtttgt ttttttaatt tactttctaa ttgtttagat
    32641 agtaaaggag ctaaagtaat acattcagta gaagaacttt tggagtttaa aaataataga
    32701 ttaaaagata ttgtagaatt ttatgcagta tatattgatg acattatgat ttgtgggagc
    32761 atggtattta actttcaaga aagagtttat catactcaat atctagcatc aaattcgaca
    32821 tataataaat attatccaat gatattttta tactataatc taataaaaga tgctttagag
    32881 agaaatttta agtatctttc ttttggaatt agcactctaa atagaggcaa atacttagac
    32941 gagggtttag ctacatttaa agaaggtttt ggttcaagtg caatttgtaa cagaacatac
    33001 tataaaaata taaataagtg agaatcatgt tttatagagt ttttagaata tagtaaatat
    33061 ctagtactct aacattacat aaaaataatt agtttataaa gaaggtttgt atatgaaaag
    33121 attaatgatt ttaggctcaa ttaagtattt tgagaatatt gttatatcag ctaaaaaaga
    33181 gggaatatat acgattgttt gtgataatag aattgatact cctgcgaaaa agatttgtga
    33241 tgaagcaata gatgttgatg tatttgattt tgataaaata aaaagtatag ctatagataa
    33301 aaagatagat ggaattttaa ctggatttac agattcttta atgaaaccat atgtttatat
    33361 tgcaaatgaa ctaaatttac catgtgtgat ttcttgcaat caacttgaat ctgtaactaa
    33421 caaggctatt atgaaaaaat attttaaaaa taatgcaata cctacaactg attactgtat
    33481 tattgaaagt ctaaaagatt tagaaaaaat acaaggatta aattttccac ttgtaataaa
    33541 acctgttgat ggttatggtt caagaggtgt atattttgta tcaaatatag aagaattaat
    33601 agaaaaattt gaatattcta aggaagcctc acaagatgga caagtaataa ttgaggaatt
    33661 ttatgaaagt gaggaaatac aaggactagc ttgggttcat aatggagagt cacatgtatt
    33721 ttatattgga gatagagagc ttgtaaacat acatacagga agagctggaa aaccagatag
    33781 attactttat ccatcaaagt attgttttca atatgaggag gaaataaaat ctatatatca
    33841 aaatataaca caggcttttg atataaaaaa tggacctctt tatgtacaaa tgttagtggg
    33901 agcatatgga ataaaggtat ctgaagtaat gccaagatta cctggaggtt gtgactatat
    33961 ggctatttct caaataacag atttagacat aggaagttta tttgcaaact ttagtgtgaa
    34021 taatgaagta aattttgatg aaataaagaa atataatatg catctatcaa aatgtgtata
    34081 tgcattacca atatacataa gacccgggac aataaaaaag ataaataata tagataaaat
    34141 agaaaactta gattatgtca ctcaatttct attactagta aaagaaggag atacaataga
    34201 attatatgaa gatgagagac aggattgtgg aagagtttat ggaatagcaa ataatatatt
    34261 tgatgcaaat ataaaaaaga aatgtattca tgaaatgata gaaatacttg atgaaaataa
    34321 tgaaaatatg atagaaaatt tttaaataag ggagtatgat agatatgaag ctaataactc
    34381 atgaacaaat tatgtcatta aacatttctc agaaacaagg atatgagtgg ataaaagaat
    34441 ctttcttaac aaaaaaagat tgtcaattac caccaaaaat aagcatggta gaagaaggac
    34501 atgtatttta caatgtaatg ccatccctaa atcataatca tatgattggt ggaataaaaa
    34561 tggttacaag attgccaggg agaaatccaa gtttaaactc agagatattt ctatacaata
    34621 tggaagatgc taaattaaag gctattttgg atggtaatta tattacagct tttaggactg
    34681 ctgctgttgc tgccttggct atcgatacat ttgctaaaaa agattttaat actattgcta
    34741 ttatgggttt aggtgtaact gcttctgcta taatgaatat gctttcatat actcttaaag
    34801 ataaaaatat aaatataaag ttacttaaat ataaagacca agcagagttg tttgtaaaaa
    34861 agtatgaaaa aaatgaacaa tttagttttg aaatatgtga tacacatgaa gaacttattg
    34921 tagattcaga tgtagttatt tctactgtga catatacaga tgaatattta tcagaattta
    34981 aatggtttaa aaaaggtgct ttattattac ctgtacatgt tatgggattt ggaaatgttg
    35041 attatctatt tgataaagta tatttagatg attataatca tttgaaacat ctagaaaata
    35101 tagatagata taaatattct tgtgaatttt atgatgtttt aagtaaacaa gctaaaggaa
    35161 gagaaaatga tgatgagaga ataatatctt acaatatagg tttgtcaata catgatatat
    35221 tatttgcaaa taaaatttac gaattgtgtg aaacactaag ctgtgaagaa atagaattaa
    35281 aagaaccaaa agaaaaatat tggatatagt ttatttcatt catatttaca atagtttatt
    35341 gtaaatatga atatgaagta atatattgtt agaaaattag catgaatagg atagataaat
    35401 ttatggataa atataattta attaggttcg tatctattgg aaacgaatat ataggaaatt
    35461 tagttgcatt agaatcaaat aaagtaatac cttttgatat aaagagagtt tattacatat
    35521 atgatgtacc aaacaatgta aagagaggat ttcatgcaca caaaaacttg gaacaagtac
    35581 tagtttgcct aaatggaagt gtgaagataa aatgttatga tggtgtaaaa gagactatat
    35641 ttaatttaca taaaaatgat gttggattat atataggaac gggagtgtgg catgagatgt
    35701 ttgattttga tgaaaaaact gttttaattt ctatagcatc tgaacattat aatgaagaag
    35761 attatattag agactataaa gaatttttaa agtatttaaa taatgtgtag ttattataca
    35821 attagtgatg tattttctta attaaataaa tttaagatag ccacatagag aatagataga
    35881 ttaggatgtg aatttattgt tggttaaatt taataattta ttaaaacagt tccaaactca
    35941 taaagaagaa tatgaacaga agattaagga tgtaatggaa agtggatatt atatccttgg
    36001 aaatgaagta aagcattttg aagaagaatt tgctaactat gtaggtagca aatattgtgt
    36061 tggtttagcc agtggtttag atgcactgat aattgctttt agagcattag gaataagcaa
    36121 aggtgatgaa gtaatcgtgc aatcaaatac atatattgct agtgttatgg gaatcagtat
    36181 aaatggagca acaccagtat ttgtagaacc aaatgaatat tataatatag attcatctaa
    36241 gatagaagaa aagataaata gcaaaacaaa agctatatta gtagtgcatt tatatggtca
    36301 agcaagtaat atgaaagata tacaagaatt atgtgataag tataacttgt atttaattga
    36361 agattgtgct caatctcatg gagctaaata tgatggaaaa atgacaggaa catttggtga
    36421 tataggttgt tttagttttt atccaagtaa aaatttgggt gcatttggag atgcaggagc
    36481 tatagtaact agtaatgagg aaatagctga taaagtgaaa attcttagaa actatggctc
    36541 agaaaagcga tattataata aatttgtcgg atataattca agattggacg aaattcaggc
    36601 aggtttatta agagtaaaac ttaaatatct aaatgaaatt gaaattgaaa aatcaaaaat
    36661 cgtagataga tatcttaatg aaattaataa tcccaaagtc atacttccaa agatagaaaa
    36721 taattgtact catgtatggc atttatttgt tttacaagtt gaaaaacgag atacatttat
    36781 ggaatactta aagaaaagtg gtatagagtc tataatacat tatccgatac caccacatct
    36841 atctgaagca tatgcttatt tgggatataa aaaggggagt tttaatatag ctgagaacta
    36901 ttcagataaa gttgttagta tacctttatt tattggtatg gaagatgaag aagttagtta
    36961 tataattaaa attataaatg aatattaaac tactcataat aatatcaaaa ataaattttt
    37021 tatatatcat ttatttaaat ttcgttaatt gacattt
//
LOCUS       DAMJVK010000034        36748 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.034, whole genome shotgun sequence.
ACCESSION   DAMJVK010000034 DAMJVK010000000
VERSION     DAMJVK010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 36748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36748
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.034"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(425..1516)
                     /locus_tag="PC414_001761"
     CDS             complement(425..1516)
                     /locus_tag="PC414_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HDF2464158.1"
                     /translation="MIEKVEEKSKIIKTSKEYKRTIIEEKDKEWTKKLNDILSRSSFS
                     NDELDKIKALIPKEILTNENVGEVVTEDGYAKPEYDEVFKSLFTLNNEYDLLVNFIND
                     ILKDAPYANRNIKPFTQIKRIIRVETDPTINYIGEKRPKLDILAEDEENNHINIEMQR
                     AFENDYLERAEYYLSRVHGRKLEEGKEYKEIGKTIGIHILNHVKYNHIEDYVNCLRLT
                     MDGHPDIYSSKTALYFIELPKIHKSDYIVNRIMLWGKLISNPSNLDVRVIAKNDSIIK
                     KALDRLKELGSNEEYLLNLKRGAYIMNKSRNFKEEIERETAIRVAKEKDYKIIIMLKK
                     NGFKLDDILNKFNDFDLSEDEIKEVYENN"
     gene            complement(2284..4203)
                     /gene="thrS"
                     /locus_tag="PC414_001762"
     CDS             complement(2284..4203)
                     /gene="thrS"
                     /locus_tag="PC414_001762"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDF2464159.1"
                     /translation="MIKVALKDGSIKEFENAISVMDVAKSISEGLARNVVAASVNGEV
                     VGLDHIIDTDCDLNLFKFEDKEGKEVFRHTSAHILAQAIKRLYPEAKLAIGPSIENGF
                     YYDIDLDHRLVPEDLEKIEAEMKKIAKEDLKIERFELPRNEALELMKEQGEDYKVELI
                     SDLPESEIISFYKQGDFTDLCRGPHLPSTKKVKAVKLQSVAGAYWRGDENNKMLQRIY
                     GTSFEKNKDLEEYLHLLEEAKKRDHRKLGKELGLFMIPEEGPGFPMFLPKGMELKNEL
                     LKFWREIHRKAGYIEIESPIILNRKLWETSGHWYHYKENMYTVKIDDEDYAIKPMNCP
                     GGLIYYNSQLHSYRDFPMRVAELGRVHRHELSGALQGLMRVRAFTQDDSHIFMLPEQI
                     KDEIKGVANLIDGIYKTFGFEYNLELSTRPENSMGSDEEWEAAENGLREALEELGLPY
                     TINEGDGAFYGPKIDFHLKDCLGRTWQCGTIQLDMQLPRQFDNTYIGQDGEKHRPVMI
                     HRVAFGSIERFIGILIEHYAGKFPVWLSPTQVKILPISDKFMDYANEVKKELFDKGIR
                     VELDDRAEKIGFKIREAQLEKVPYMLIVGEKEVADNNVSVRSRDKGEIGSIKLDEFIA
                     SISKEIESRESIIQD"
     misc_binding    complement(4267..4505)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(5117..5719)
                     /locus_tag="PC414_001763"
     CDS             complement(5117..5719)
                     /locus_tag="PC414_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HDF2464160.1"
                     /translation="MDNLIRILILAVIGGFIGYVTNVVAIRLIFRPIEPIKIPILNIE
                     IVGLIPKRRAEIAANVGEIIQEEFLSMDEILANIITDEDKEEVVRYIKARVKIIIHEK
                     VSFIPSGIKNMIQDYLGEIIESEVKQSIDELSKNIINKANERIDIQKMVEDKINELDL
                     YELEEIIIRIAKKELKHIEFLGLVLGFLIGIAQGLITMFI"
     gene            complement(5899..6717)
                     /locus_tag="PC414_001764"
     CDS             complement(5899..6717)
                     /locus_tag="PC414_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sporulation protein YtxC"
                     /protein_id="HDF2464161.1"
                     /translation="MVEKDNISSMKFLRNNNVNDRLIYTNDIVEVVIDSAKQEVLNKK
                     ILNGTKAEQRALNIDEISTEITSMIISIMKEKLLKEYILKCYGRTYPEDKNNIYIYSL
                     NIFAKKEIFMRETVFTRVRNYIEENDFINVEGFIRFRMREFMKYISAIGDIAVEEYLI
                     KKDQDEFIRVLKYFIDTQEEKIDLLKVHIMEDNTFVLYDKNGNKIDSIDDEEIINMVI
                     RENLNYEDFLISTLLTLCPKKIEILDLLNNNCSKEIIDTVEAIFENKVSIIMEN"
     gene            complement(6720..7481)
                     /locus_tag="PC414_001765"
     CDS             complement(6720..7481)
                     /locus_tag="PC414_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464162.1"
                     /translation="MEFTREPFILDDDLCLALVDKRISYLMENELKLRNIEIIKTIEC
                     KELYESIKYHPDICICNLGKGDIIVAPNVYNQYKELLSRYKFNIIKGEANLLNRYPYD
                     IPYNIAIVGDYAIHNFKYTDKAILDYIENNKLKKINIEQGYSKCSICIVDRTSIITSD
                     KGIWKSMKNTEIECLLIEKGHINLFEMNYGFIGGCTGLISKDKLAFCGDVKKHPDYER
                     IKSFVESKNKEIVTLSCENLLDIGSIVPLMTRKER"
     gene            complement(7971..9083)
                     /locus_tag="PC414_001766"
     CDS             complement(7971..9083)
                     /locus_tag="PC414_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2464163.1"
                     /translation="MKIKRILMACFVVVFTIFILAICNFDFGFDKVNKNNSNIDSAKS
                     KQSESKSLLTSAEKDKSNSKSKNKEDDTEDDSYVRKTIGVLLDTENLNNESYNNNVLS
                     ALKRADEDFDVRIKLEKPKTSKSFKESIEKLHKYEPDFIIAVGARFAEPIKEAATKYP
                     KQQFAIVDYTYKVQPANVISVSFQDNVTGYLAGLIAGKMTDTGKVGFIGGVEGSSRDK
                     YESGFRKGLKTSNKDAELLVKYSNIFENPESARTIAKEMRDSDRDVVFSATEDDSKRA
                     IQVARENGGKVIAINKDQYDMAPDNVISSIIKDVEQPVYELIKNVAKDGFKGSKVMEF
                     KIKDGELGLTSKSSRNIPPDVLEYIKKEYNKVKDTY"
     gene            complement(9209..10213)
                     /locus_tag="PC414_001767"
     CDS             complement(9209..10213)
                     /locus_tag="PC414_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2464164.1"
                     /translation="MRFKRILILMLTVAMIAGMLVGCANNPSNNPSSSKDNKSDISDK
                     SNSKKSVSMILDIEGTNNEAMNNSALLALNNAQKKLNIDTNKVESDDSSTFSNSIDIL
                     CNDNYDLIIAVGARFAKPLEMVAKKYPKQQFAIIDYEYDKQPSNITSISYEDNKSGYL
                     AGLIAGKMTESDKVGFIGGTKSSSRDKFESGFREGVKFSNSSIKDISVEYADVFKDSK
                     SVESIAKKMMDNGVDIIFSTTEDDSKAVIDAVRAKNKKVIATNKDQHELAPENVISSI
                     VKDFENPTYNLIQSFVKGNYKGGKVIENTVKSGSTGLAQNSSQNIPPDVLEYVNKNNK
                     "
     gene            complement(10501..11349)
                     /locus_tag="PC414_001768"
     CDS             complement(10501..11349)
                     /locus_tag="PC414_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDF2464165.1"
                     /translation="MKNLKIICDSLSDVPKDLLDKYDIDIVSLTVILEEREYKDRIDI
                     SGDEFYKRLREENVYPKTSQATYAQFKEIFEKYTNEDREILYIAGSATATGTYQSAVM
                     AKNDVDGKIYTYDSNLLCMGTGLLVIKAGELASEGKSVAEILPVLDELKEKAFVIFSV
                     DTLEYLQKGGRISSTKAAIGSILNIKPILEIRDGLVSQISQVRGKKNVISKMMELIKS
                     NCGDDLSDQIVYIGYSDDFKEKEQLSKVMQDELRPKEIRFFQIGVCIGAHAGPGVTGI
                     ICLKNK"
     gene            complement(11385..11855)
                     /gene="trmL"
                     /locus_tag="PC414_001769"
     CDS             complement(11385..11855)
                     /gene="trmL"
                     /locus_tag="PC414_001769"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HDF2464166.1"
                     /translation="MSLNIVLVEPEIPQNTGNIIRTCAATGSTLHLVKPLGFSLDDKH
                     LKRCGLDYWDIADIQYYDSFEELQEKYPNSKYFFSTTKAKQSHSDVKYEENCFIVFGK
                     ETKGLPEPLLKANMDTCIRVPMLDVEKARSLNLSNSVAIVVYEALRQIGYPNMR"
     gene            complement(12049..13218)
                     /gene="nth"
                     /locus_tag="PC414_001770"
     CDS             complement(12049..13218)
                     /gene="nth"
                     /locus_tag="PC414_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HDF2464167.1"
                     /translation="MKNVNLKEINEKDVILEEEINKKGMDKKNIIEKEEINKKGIDKK
                     DIIEKEEINKKGIDKKDIIEKEEINKKGMNKKDIIEKEEINKKGMNKKDIIEKEEINE
                     KGIDKMIGKKDEIIDEKDIENFMDIEEDKKSIKVSKKSTKKGETEKKTKKSSSNKATQ
                     KTSKETKSKSKIKNESANEKDVNKILDELEKLYPDAKCELNYGTAFELLIATILSAQC
                     TDVRVNKVTSELFKKYNTARDFANLSIEEISKEIKSCGLYKSKSQKIKDTSEQLCELY
                     DGEVPDSLEKLIKLPGVGRKTAGVVLSNAFNHPAIAVDTHVFRVSNRIGIVDEPNPQK
                     TEFALMEAIPKERWSHSHHVLIFHGRRMCKARNPECASCPIKEDCNYYKELNETK"
     gene            complement(13416..15653)
                     /locus_tag="PC414_001771"
     CDS             complement(13416..15653)
                     /locus_tag="PC414_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2464168.1"
                     /translation="MKYNNTSEINPLDYILGQERAVEAMELGLKINNPAYNIYIAGEP
                     GTGKSTYALKVLNEYASKKNTHKDWCYIYNFENPREPIIVELEKGFGRELKKDMEKLI
                     ESLLEDFKNAFESENFEIEKNKLLDEYEIEKDMLLKQIKKYGEEKGFKLKQSKMGIVF
                     IPLKEEEDESDESDEEFYKAKRELENMAIQVVYKIRNLEEIAEKAVLELEEEIAKLVV
                     EPHIKRLCEKYENYDKIQTYLENIKKDIIEYMYLFYLDEEELKDKYDKEHFIKYKINL
                     FVDNGSSKNKESAPVVVEINPSPANLFGKAEYDYANGNIKTDFTKLLSGAFHRANGGY
                     LVLYADQLLKYTMSWEILKKTLQTKKVILETQTAIKPENMPLDVKLVLIGSHYIYDIL
                     YRYDEDFEKYFKVFVDFDSEMDKNEDNEEGIARFIAYQCDKNNLRHFTKSAVEEVIKF
                     STRLSGDLEKLSTKFNKIMEVVIEGSAYAEFRNSEYTEQCDVKKAISEKRKRINRVET
                     HMDESIENGFTLIETEGRKVGVINGLSVLSTGEYSFGRASRITATTSPGSKGIVNIER
                     EVNMSGSIHNKGVLILGGYLSENFAQDLLLSLNAYVCFEQNYGGIDGDSASSAELYVL
                     LSSLSGVSIKQNIAVTGSINQKGDIQVVGGISEKIEGFYSICKTKGLNGEQGVIIPRK
                     NQRNLVLSDEVNDAVRDGKFKIYTVERVEEAIEILTDVKFEEIKKLVKEKLISFSKIQ
                     TVSKE"
     gene            complement(15759..16673)
                     /gene="queG"
                     /locus_tag="PC414_001772"
     CDS             complement(15759..16673)
                     /gene="queG"
                     /locus_tag="PC414_001772"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HDF2464169.1"
                     /translation="MDNKELKAFCNLIGLNCVGVAGVDKYDNLEKILKDRQQKGYLTG
                     MEEPIIENRINPRHIMKDANSIIVCAFPYHINRQEKNKNSNLSKYCHGKDYHIVVKDF
                     LQQICDYISKTVTDFKYKLFADNGPLVDRYLAYLSGIGYFGINNNIITDEYGSYVFIG
                     YIVNNYKFEEDKPLEKSCFKCGKCVKYCPGNAILGNYDMNPKKCLSYITQKKGDLSEK
                     EKVVLKNGKKVFGCDICQEVCPHNVEIPTTHILEFKENIIDYLNYDEIQNISNKEFKR
                     KYGDRAFSWRGRNIIKRNMEIILDKYDD"
     gene            17695..18099
                     /locus_tag="PC414_001773"
     CDS             17695..18099
                     /locus_tag="PC414_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDF2464170.1"
                     /translation="MIKITIPGRPISKSNFKLHNINGQAWMPSKGKYSKYLAYENMIA
                     GFINQQYQGETVEENLITVLKLFFPNKRMGDLHNYPKSICDGIEKSGIIKNDKQLKPV
                     LLFDFIDKDNPRVEIELYPVSKYDISYNIFEK"
     gene            complement(18356..19351)
                     /locus_tag="PC414_001774"
     CDS             complement(18356..19351)
                     /locus_tag="PC414_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDF2464171.1"
                     /translation="MRNLGNTNMKIKRVGFGGIPIQRITQDDTNLVINELEKQGINFI
                     DSARGYTISEEAIGIAIEGKRDKFFLATKSMSRDYDSMKRDVEISLNNFKTDFIDLYQ
                     FHNVKEEEYDNLFKDKMAYSALLEAKEQGKIKHIGITSHNLNTIEKAIEDGKFDTIQF
                     PYNIVEGQADEVFKKAHEKGIGIIVMKPLAGGALDNATLAIKYILSKEYIDVVIPGME
                     SVEQVRQNVAVLENLVLDEKDNKEIEEIRNSLGKKFCRRCEYCMPCAVGINIPLSFLC
                     EGYYTRYGLKEWAKEKYEVMDVKPTECIDCGLCESRCPYELPIREMLKTVVEKLG"
     gene            complement(19635..20471)
                     /locus_tag="PC414_001775"
     CDS             complement(19635..20471)
                     /locus_tag="PC414_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDF2464172.1"
                     /translation="MIKLGTHLSIAKGFANAAETAVYIGANTFQFFSRNPRGGNAKEF
                     DEKDIIKFQEIRKENNFAPLLAHAPYTMNLGGTKDDVYEFATRVIDEDIRRMDSLGIE
                     YMCFHPGSHVGGGVDFGIDRIVKGLNNAIKGDENITILLETMSGKGTEIGFKFEQLKS
                     IIDKIEHKEKIGVCLDTCHIFSAGYDIVNDLDGVLEEFDKVIGIERLKTVHLNDSMMP
                     FGDKKDRHAPIGEGKIGLKALIDFMEHPSLKHLPFFLETPFDDEGHKRELKMIKEILS
                     SK"
     gene            complement(20959..21522)
                     /locus_tag="PC414_001776"
     CDS             complement(20959..21522)
                     /locus_tag="PC414_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2464173.1"
                     /translation="MKEVLVLRDLECIKAIAHPRRIDILKAFDKSPLSAKQLSQLLEE
                     PHAKINYHIKMLYKVGILELVEEKIKSGIVEKYYYPSAKNVVIGNRILNFSLDNGEEK
                     EELYISKFENMSEVFYKAAEEDVLENENIVDYHDISLTHDELVELSDTMKSKIDEILN
                     KRQHNVEGSKYDIAMVIVPTLEEECPS"
     gene            complement(21620..22549)
                     /gene="glsA"
                     /locus_tag="PC414_001777"
     CDS             complement(21620..22549)
                     /gene="glsA"
                     /locus_tag="PC414_001777"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HDF2464174.1"
                     /translation="MNTLDETLLKEIISSNKKYTNYGQVASYIPELKNARRNDLGICI
                     IDSENNLYSAGNCSTKFTIQSISKPIVLAMALMDNDWEDVFSNVGMEPSGDPFNSIMK
                     LEINDTKKPCNPMINAGAIVTTSLINGSCLEEKEERMLSFFRKLAKNDNIGINYDVYK
                     SEKMTGDRNRAMAYLLKSDGFIRGNVEDVLDLYFKQCSIEIDSVDLARIGINLANYGV
                     DIENGEHLMSEMVSRIVKTFMMTCGMYDASGEFAIKVGIPAKSGVGGGIMASVPGRMG
                     IGVYGPALDKKGNSVAGVKVLEELSNKLKLNIF"
     gene            complement(23508..25175)
                     /locus_tag="PC414_001778"
     CDS             complement(23508..25175)
                     /locus_tag="PC414_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HDF2464175.1"
                     /translation="MKKINLKIEGKDKEYSLEENSPGIRLGDIAKEFCDEHKGYITLA
                     VVDNKLKELNCRVKKDCEINFLDTTNEDGERVYFRVMSFIFVMACREIFWDSRVTIEH
                     SLSDGLYCEVHIDRKLKEADVENIKNKMKEIVNNDYVIEKIEVTRNEAISIFENNEMY
                     EKAELLKYKEYDSAKVYKCRNYIDHFYGYMLPSTGYIKSFDIKLYNGGVIILGPSEED
                     KTLPMKFVPQPKLSSVYYEAEEWSRLMGIDKVVSLNKIIENNEYGDIIRTFEALHEKK
                     LSQIADMIKDGNKRVVLIAAPSSSGKTSFAHRLSIHLRVNNLNPISISLDDYFIDRKH
                     TPLDEYGNYDFESIYAIDLEKFNLDLKKLLAGEEIDDIRFNFKEGKREYTGKKIKIDS
                     NQPIILEGIHGLNPILTSSIPDEDKFKIYISALTQINLDDHNRIPTADLRMIRRIVRD
                     YNFRGYSADNTILQWASVRRGEKKNIFPYQEEADAIFNSACVYELAVLKRFAKPLLEE
                     IKEDNPAHIEATRLLKFLQYFVELDDTSDIPGISILREFIGGSKIVD"
     gene            complement(25249..26004)
                     /locus_tag="PC414_001779"
     CDS             complement(25249..26004)
                     /locus_tag="PC414_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HDF2464176.1"
                     /translation="MAVGIASLLFNIEEALNICESIKQITHLEIGIDNISECSELCKY
                     KERISKLGLSIGIHLPMELNTCENIEYIRNSWINFIEKIEFELKGFDLRYFNLHLGYV
                     MTNRVRKNRDKYLGNSVDFLDKLNTNSYVCIENTYSKGGDFSNIGNISYDFEYIFKRI
                     KNSKICFCYDTGHCLIDEDAYVKNLKDKIRLIHLSDNDGINDTHVGIGRGILSEEGIK
                     EILTLDAEYLVLEINYEDIEDTISKLNNIVGEG"
     gene            complement(26014..26550)
                     /gene="lepB"
                     /locus_tag="PC414_001780"
     CDS             complement(26014..26550)
                     /gene="lepB"
                     /locus_tag="PC414_001780"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDF2464177.1"
                     /translation="MNETIKEEIVEWIKIIITALFFAFIITRFIKPTLVNGESMYPTL
                     KSHDYLVANRMTYKLSEPKCGDIMIFKTDLLQENGRKKELVKRVIGVPGDHLKIKDSK
                     VYINGKLLNEVSYIHDNYTEGDIDMVIPKGKVFAMGDNREVSLDSRYKEVGLVDEENI
                     KGKVILRVFPFTDIGIFE"
     gene            complement(26716..27252)
                     /gene="lepB"
                     /locus_tag="PC414_001781"
     CDS             complement(26716..27252)
                     /gene="lepB"
                     /locus_tag="PC414_001781"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDF2464178.1"
                     /translation="MGEAVKKEVVEWIKVIVIALVLAFAITRFIVPTIVKGESMYPTL
                     VERDYLIVNRIAYKVGEPKYKDIIVFKTDLTEENGKKKDLVKRVIGVPGDHVKIQDSK
                     VYVNDKLLDETSYIHNNRTDGDIDIVVPEGKLFAMGDNREKSLDSRYDEVGLVDEHTI
                     LGKVLVRLYPFSKIGTID"
     gene            complement(27352..28662)
                     /locus_tag="PC414_001782"
     CDS             complement(27352..28662)
                     /locus_tag="PC414_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HDF2464179.1"
                     /translation="MTELPKEFLEDIEEILKDEYDDFIKSYEESKTTGLRINTLKIDK
                     KKLFDLELFDLNQIPWAEEGFYYDDKIDRPGRNPLHEAGAYYLQEPSAMSVIPKADIK
                     EGEKVLDMCAAPGGKSTYILSKLNDTGLLVSNEINASRIKALGENLERFGARNCIITN
                     TDSNSLRKTFNGYFDKVIIDAPCSGQGMFRKDEVAISDWSYAKVVECQSIQKEIIRDG
                     YKMLKKDGILIYSTCTFSREENEHVINEFISEYPHAELIEMERLWPHKIKGEGHFVAK
                     IKKLEEEECNVKELKIKNLDKEIKDYREFEKKFLKVDFYSNKKNKFYLRGENLYLLPE
                     IYPDTKKLKVLRYGLHLGVLKKNRFEPSHALSHYLKVEDVKNVENFSVQSESILKYLK
                     GDVVNSNDSRGWVLVSVEGIPLGWGKESSGVIKNHYPKGLRKVF"
     gene            complement(28741..29457)
                     /locus_tag="PC414_001783"
     CDS             complement(28741..29457)
                     /locus_tag="PC414_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HDF2464180.1"
                     /translation="MKLDLFLLAIIPILIGMFWIRSKDRYCREPLIHLIKFFLIGAFL
                     SVIIILLENLLMKFNVFEGYSELIYVSFVVAGLVEEGVKALILIPALIKEKHFTEKLD
                     GIIYSVFLALGFATIENMVYIFSESRNLALQVGINRAVISIPAHVMFAITMGYYISKY
                     KFEGNKNKRREYLFMAVLIPILLHGVFDFILMIEYRWAIILLIVYVIILWKINLDKLE
                     KYMNHSKKVFFGNLRKKKKK"
     gene            29702..30973
                     /gene="sleC"
                     /locus_tag="PC414_001784"
     CDS             29702..30973
                     /gene="sleC"
                     /locus_tag="PC414_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex-lytic protein SleC"
                     /protein_id="HDF2464181.1"
                     /translation="MQDGFLTVSIIDATNNRPIQNAVVNIYSMSNGSQSSSTLYQNLR
                     SNESGQVTGLVLPAPDVDYSLQPSDVRPYSQYIVEAIADGYETVVIEGTQLLATIEAR
                     QGVPMSPRTRSKRSFSRQSELIFDIGEHTLYGTYPPKIPESNLKPLPPPTGFVVLDNP
                     VVPEFIVVHDGLPEDSSAPNYWIPFKEYIKNIASSEIYSTWPEQTIYANVIAIISFTL
                     NRVFTEWYRNKGYNFTITSTTAYDHKFINNRNLFEPINVVVDAIFNTFIKRPPTSRQP
                     LLAQYCDGQKSQCPDQMTQWGSKDLGDQGYDYESILRYFYGDEIVFERAPIVSGVPVS
                     FPGTTLQVGSSGQYVRTIQNQLNAISNSYPAVPKVIEDGIYGTDTENAVKIFQGIFGL
                     PQSGVVDFKTWYEISRVYVATTRIASLNPLI"
     gene            complement(31399..32055)
                     /locus_tag="PC414_001785"
     CDS             complement(31399..32055)
                     /locus_tag="PC414_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464182.1"
                     /translation="MKKGDFIWSGVLICIIAVLAIPSSREVFMEATGAHPYIGGFLKF
                     AVLATMGDLLGVRILYKDWKIPVGVFYRAIVWGIIGVVTTYGMSIYSSGVGQAQVDGM
                     LPFEGIAFANALFKSATMNILMSPTVFLFHKVMDCFIDKKYEVGKGIKVKLKDVVNIV
                     DWNAYLGFSVLKTIPFFWIPCHTIVFLFPAEYRVIASAFASIALGLILALANKSKVAV
                     "
     gene            complement(33167..33577)
                     /locus_tag="PC414_001786"
     CDS             complement(33167..33577)
                     /locus_tag="PC414_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464183.1"
                     /translation="MKIKKSLISAMMVSAMVLGGTGAVFANGNDSSNIALPKFESLIK
                     SGITDPTELEKYGVEHYTYEEYAKHIQELKDYAKDPNAVKDVSQKDLEETIKKMEQEL
                     EKIKTEGLKIMKPITIENEDGSQISIAYNYAAID"
     gene            complement(33793..35175)
                     /locus_tag="PC414_001787"
     CDS             complement(33793..35175)
                     /locus_tag="PC414_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HDF2464184.1"
                     /translation="MFIKLLEMSFTSGVLLLIVIFIRACLFKKLPKKVFVLLWLIVLF
                     KLLIPVSLNSGFSIYSIIESTFDVELGNTSTENYTLKKASDSTGKNNSDEFEIMSNIE
                     NIMMLEMLGILSFIVTIMFSILLCFRYFKIINLFKTSIHIKNHEFIKKWMCKNSRFRR
                     FIVCISEFTRTPLTSGILKPRIILPKQMNYANNQTLDFVLTHEMVHINSFDNLLKLFM
                     LVALVVHWFNPLVWVMFFLLNKDIEFSCDEKVMSILGEEYRADYATTLISLAEENNPF
                     SMTICSGFGKTKIEKRIVNIMKFKKSTYVTFLVSTVLVCGTGLVFATENKEINEVESF
                     GLTKNEQSDKIGFSISSKSTDLYSPKVESDILVSKAKSDGETHFSTAKVSVIEFREHE
                     ESSMSKVTNSENSEETYVNKSSNSKDVLYTDSGEVSIERSGYNDNRFVILRKGNLLAV
                     NKEQYRTWIE"
     gene            complement(35186..35545)
                     /locus_tag="PC414_001788"
     CDS             complement(35186..35545)
                     /locus_tag="PC414_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2464185.1"
                     /translation="MEIKLFDSELKVMEVLWERGESKAGELVKILKEETGWNRNTTYT
                     VIKKLVSKGAIERIEPNFVCKALISKEEVQKQETETLINKMFGGSSEKFLSAFINKEN
                     LSSDEITKLKQIVEKLK"
     gene            complement(35750..36529)
                     /locus_tag="PC414_001789"
     CDS             complement(35750..36529)
                     /locus_tag="PC414_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HDF2464186.1"
                     /translation="MVSRQELKRVSKAQLSGNWGICAISMAVYIALIVLISIVGLPFE
                     PLTTIVVAVIAFCVSAGFTCMFLKITKDEKVKIGDIFVSGRIYLRSFGFYLFESIIVY
                     IYSFIITIIGAFLSFGVLLTSGAIEYLSSDNVGIGTIISTGITLVLIILVLMLPVYIL
                     LLYVSQATFLICEDKDNMGVFKSMGASLDMMKGNVLKLFVLNFSFIGWYILSIATLGI
                     GLLWLIPYVGVTNCNFYRQLVKENPDVEDILLDKEKSYTMY"
BASE COUNT        12013 a         6673 c         3439 g        14623 t
ORIGIN      
        1 tacattattt ctataataaa atcttataaa tattacaata aaatattttt ttccatgata
       61 taccttgata aaatctctgc ttctttaagc agtaactttg agttgaatca ttatagtgat
      121 ttataacatt tatagaaatg ctgttataga tttatattta aacactacaa atggaaattt
      181 aactattata aaaaacaatc attagataaa atgattctta caacaaaagc atttctattg
      241 tagatttatt agtaaataat aaatctacaa tagaaaaaac tagaagtaca gtatcataaa
      301 aatataatta actgtacttc taaattattg tttttaaaat aaattttcat tcattaaaaa
      361 atacatataa atatttccag actccgattc agcaaactta tataagttca ctttttattt
      421 atcatcaatt attttcatat acttctttta tctcatcttc acttaagtca aaatcattaa
      481 atttatttaa tatgtcatct aatttaaaac catttttttt caacattata attattttat
      541 aatctttttc ttttgcaact ctaattgctg tctctctttc aatctcttct ttaaaatttc
      601 tacttttatt catgatataa gcacctcttt tcagatttaa taaatattct tcattgctac
      661 ctaattcttt taatctatca agtgcttttt taataataga atcattctta gctataactc
      721 ttacatctaa gttagaagga ttactaataa gttttcccca tagcataatt ctatttacta
      781 tatagtcaga tttatgaatt tttggaagct ctataaaata taatgcagtt ttactagaat
      841 aaatatctgg atgcccgtcc attgttaatc tcaggcaatt tacataatct tctatgtgat
      901 tatattttac atgatttagt atatgtattc caatagtttt tccaatttct ttatactctt
      961 taccttcttc taactttcta ccatgtacac gactaagata atattcagct ctttccaaat
     1021 aatcgttctc aaaagctctt tgcatctcaa tatttatatg attattttct tcatcttcag
     1081 ccaaaatatc aagtttaggt cttttctctc caatgtaatt aattgttgga tcagtttcaa
     1141 ctctaattat tctctttatt tgagtgaacg gtttgatatt cctgttagca tatggagcat
     1201 cttttagaat atcatttata aaatttacaa gtaaatcata ttcattattt aaagtgaaca
     1261 atgatttaaa tacctcatca tactcaggct tagcatatcc atcttcagtc acaacttctc
     1321 caacattttc attagtcaaa atctcttttg gaattaatgc cttgatttta tctaattcat
     1381 cattagaaaa actacttcta ctaagtatat catttaattt tttggtccat tctttatctt
     1441 tttcttcaat tatagttcgc ttatattctt ttgatgtttt aataatctta gatttttctt
     1501 ctactttttc tatcatttca acacctctct ttttcctctt ttgtatttat catttcttga
     1561 aattaaacta agtcactacc tcttttaaaa gttcttgctc cataaacact ttcttaaact
     1621 ctaatgtctg tgtatgtact tgacattctc tctctatcta aagttaaata aattaatcta
     1681 tgtatagagg ataagtccaa aaataacata aaacactaat taatcattta taaagcatta
     1741 attaatcact gacaatgctg ttattgtcac cacatagcac caacatagat tcttctatta
     1801 tagggcaatt atctatgaaa aatagactca taagattaga aaatgtactt aaattttagt
     1861 tcatttaaat attcattttg aagaaagaaa tttaacttta gactaaaaag gaaatatttc
     1921 aagaggatta agaaattttc ttaaagggat aataattttt atgaaaatgg aacaaatatg
     1981 aaaataaagt tatgattttt acagccataa ccttaccaac aagcacttaa ttatcactca
     2041 aaaaataaac ttttatttaa agaatgcaca taaatatttc catgttcaaa acacttctat
     2101 gatatattta ttaataaatc taccatacaa aaaactagag gtacattaat cataaaaata
     2161 tgattaactg tacctctaat tatactcatc ttttcataaa tttaatcact atgcatcaac
     2221 atatcaacat attgattaaa cttatcttat ataacaatat cttagctact aatataatat
     2281 atattagtct tgaataatac tttctcttga ttcaatttct tttgaaatac tagctataaa
     2341 ttcatctaat ttaatactac ctatttcacc tttatctctt gatctaacag aaacattatt
     2401 atcagctact tctttttcac ctactattaa catgtatggc actttttcaa gctgagcttc
     2461 tcttatctta aatccaattt tttcagctct atcatctaat tcaactctta tacccttatc
     2521 aaataactct ttcttaactt catttgcata atccataaat ttatcagata taggaagtat
     2581 cttaacttga gttggagata accaaactgg gaatttacca gcataatgct ctattaatat
     2641 acctataaat ctttctatac taccaaaagc aaccctatga atcataactg gtctatgctt
     2701 ttcaccatct tgaccaatat aagtattatc aaattgtctt ggaagctgca tatctaattg
     2761 gatagtacca cattgccaag ttcttcctaa acaatctttt agatggaagt ctatcttagg
     2821 accatagaat gctccatcac cctcattaat agtatatggt agacctaact cttctaaagc
     2881 ttctcttaat ccattttctg ctgcttccca ctcttcgtcg cttcccatag agttctctgg
     2941 tcttgtagat aattctaaat tatattcaaa tccaaaagtt ttgtatatac catctattaa
     3001 gttagctact ccttttattt cgtccttaat ttgctctggt aacatgaata tatgtgaatc
     3061 gtcttgagta aatgctctaa ctctcattaa tccttgtaat gcaccagata actcatgtct
     3121 atgaactcta ccaagctctg caactctcat tgggaaatct ctatatgaat gtagttgaga
     3181 gttatagtaa attaatccac caggacagtt cataggtttt atagcataat cctcatcatc
     3241 tattttaact gtatacatat tttctttgta atgataccag tgtcctgaag tctcccataa
     3301 ttttctattt aatatgattg gagattctat ctcaatatat ccagcttttc tgtgtatttc
     3361 tctccagaat tttaataatt catttttaag ttccatacct tttggtaaaa acatagggaa
     3421 tcctggacct tcttcaggta tcataaataa tcctaattct ttacctaatt ttctgtggtc
     3481 tctcttttta gcttcttcta gtaaatgtaa gtactcttct aagtctttgt ttttctcaaa
     3541 agaagttcca tatattcttt gaagcatctt attattttcg tcaccacgcc aatatgcacc
     3601 tgctacactt tgtaatttaa cagcttttac ctttttagta gatggtaaat gtggtcctct
     3661 acataaatca gtaaaatcac cttgcttgta gaaagatatt atttcacttt caggtaaatc
     3721 agaaataagt tctaccttat agtcttctcc ttgttctttc ataagctcta aagcttcatt
     3781 tctaggaagt tcaaatcttt ctatttttaa atcttcttta gctatttttt tcatttcagc
     3841 ttctattttc tctaggtcct ctggaactaa tctatggtct aaatctatat catagtagaa
     3901 tccattttct atacttggtc ctatagcaag ctttgcttct gggtaaagtc tttttatagc
     3961 ttgagctaat atatgcgctg atgtatgtct aaaaacttcc ttaccttctt tatcttcaaa
     4021 cttaaataaa ttcaagtcac aatcagtatc tataatgtgg tccagaccta caacctctcc
     4081 gttcacagac gctgctacca catttctagc taatccttca ctaatggatt tagctacatc
     4141 cataacagaa atagcatttt caaattcttt tattgagcca tcttttaaag caactttaat
     4201 cattgttttt tcctccttat tgaaataatt tttagatttt ttgtaataaa aaatcgcccc
     4261 aaatatgttt gggacgatat taattcgcgg ttccacccaa gttggcgtat acaagtatat
     4321 acaaccctct tgatgactat aaggtcgtca cccgagcact ttcacgtata gtataaatac
     4381 aaatactgct aataagctca gctccaaggt agttttcaaa tagacctatt acatgggctc
     4441 tcagccatag acccaaattc tctgagaatg gtttctactc tactcttcct cttcaacact
     4501 atgattttta atatattttc attataacag atagttatac atcaatcaac attaatgtaa
     4561 tatattaatt acccattatt tttcatctca atcagctgta aaccaatatt cttatatttt
     4621 tatattaaat taatctcttg atttatagtt atattataat attttcataa atttaaaatt
     4681 tttaaaacgt atccacatgc atgatagata tgactagata aatactaaaa ctaattttta
     4741 ttaaaactaa cttctaattt ttgtactctt tttaggtttt gtatacttaa atacaatcaa
     4801 tacttaatat ggttaataag tagtacagta aatacttaaa tactattaat aagtaacaca
     4861 atcaaataaa taaatacaat taatatctaa atgctattaa taagtaattc actcaaatag
     4921 gtaaatacaa ttaatatcta aatactatta ataagtaatt cactcaaata ggtaaataca
     4981 attaatatct aaatgctatt aataagtaat ttactcaaat aggtaaatac aattaatatc
     5041 taaatgttat taataagtaa ttcactcaaa taggtaaata taattagtac ttaaataaat
     5101 gctacttctc atatatttaa ataaacatag taattaaccc ttgagctata cctattaaaa
     5161 accctaagac aagccccaaa aactctatat gttttaattc tttcttagct attcttatta
     5221 ttatctcttc caattcatat aaatctagtt cattaatctt atcttcgacc attttttgta
     5281 tatctatacg ttcatttgct ttatttatta tatttttact caattcatct atactttgct
     5341 taacttcaga ctcaattatc tctcctagat aatcttgtat catgtttttt ataccactcg
     5401 ggataaatga aactttctca tgtattataa tttttaccct tgcttttatg tatcttacaa
     5461 cttcttcctt atcttcgtca gtaatgatat ttgcaagtat ttcgtccata gaaagaaatt
     5521 cttcttgaat tatttctcct acatttgctg ctatttctgc tcttctcttt ggtataagac
     5581 ctactatttc tatatttaaa ataggtattt taataggctc tattggtcta aatatcagtc
     5641 ttatagcaac tacattcgta acataaccta taaatccacc aataacagct aatatcagta
     5701 tcctaattaa attatccatt ataaaattac tccttatcaa ttgtatattt gccagctata
     5761 ttgcattaat caagtagata ttctttatac ttaataactc tagttatact atagctttat
     5821 atacaattat aagtcttttg tgtcgattta aaaagaattt ttttaatttt atatggatag
     5881 cttttatatg cttagattct agttttccat aattatacta actttatttt caaaaattgc
     5941 ttctacagta tctataattt cctttgagca attattattt aacaaatcca atatttctat
     6001 ttttttggga catagagtta ataaggtact tattaaaaag tcctcataat ttaaattttc
     6061 tcttattacc atatttataa tttcttcatc atctatactg tctatcttat ttccattttt
     6121 atcgtataaa acaaatgtat tatcttccat tatatgaact ttaagcaaat caatcttttc
     6181 ttcttgagta tctataaagt attttaaaac tcttataaat tcatcttggt ctttctttat
     6241 taaatactcc tcaactgcaa tatcacctat agcagaaata tatttcataa attctctcat
     6301 tctaaatcta ataaaacctt ctacatttat aaaatcattt tcttctatat aatttctcac
     6361 cctagtaaat actgtttctc tcataaaaat ctctttttta gcaaatatgt tcagtgaata
     6421 tatgtatata ttatttttat cttcaggata tgtacgccca tagcatttta gaatgtattc
     6481 tttcaaaagt ttctctttca ttatacttat tatcatacta gttatttccg ttgatatctc
     6541 atcaatattt aaagctcttt gttcagcttt agtaccatta agtatttttt tatttaacac
     6601 ttcttgcttt gcagaatcta tcactacttc tacaatgtca ttcgtatata ttaatctatc
     6661 gtttacatta ttattcctca aaaatttcat ggaagaaata ttatccttct caaccaaagt
     6721 tatcgctcct ttcttgtcat gagaggaact atagaaccta tatctagtaa attttcacag
     6781 ctaagtgtaa ctatctcctt gtttttactt tctacaaaac tttttatacg ttcatagtca
     6841 ggatgttttt taacatctcc acaaaaagct agtttatcct tagaaatcaa ccctgtacat
     6901 ccacctataa atccataatt catctcaaat aaatttatgt gacctttttc tatcaataaa
     6961 cactctattt ctgtattctt catacttttc caaatacctt tatctgatgt tattatagac
     7021 gttctatcta ctatacagat tgagcattta gaatagccct gctctatatt tatcttttta
     7081 agtttattat tttcaatata atctaatatt gctttgtcag tatacttaaa attgtgtata
     7141 gcatagtcac caactatcgc aatattgtaa ggtatatcat aagggtatct atttaataaa
     7201 ttagcttctc ccttaattat attaaattta tatctactta ataactcttt gtattgatta
     7261 tatacattcg gcgcaacaat tatatctcct tttcctaaat tacaaataca tatatccggg
     7321 tgatatttta tgctttcata taattcttta cattctattg tttttattat ctctatattt
     7381 cttaatttta attcattttc cattagataa ctaattcttt tgtccaccaa agctagacac
     7441 aaatcgtcat ctagtataaa tggttctctt gtaaattcca ttttttactc ctttttcttt
     7501 tttttgataa attttttatg tgaatataaa taaagtgtgt agcagaattt ttctgcccac
     7561 acacttattg ttataatttt atatagttat tctaagctta attagtatag ctttgtacag
     7621 ttggttcaaa tttaataaat atatcttttt atcactaatc taaacttaag cagtatattc
     7681 cgtacagtta gcacaaattt aataaatata tctttttatt gctaatctaa actcaatcaa
     7741 tatattttgt acagttagac taaatttaat aaatatatcc ttttatcacc aatctaaact
     7801 taagcagtat attccatact gttaacacaa atttaataaa tatatctttt tatcactaat
     7861 ctaaacttaa tcaatatatt ttgtacagtt agcacaaatt taataaatat atctttttat
     7921 tgctaatcta aacttaagca atatattttt ataatcttct acatcaataa ttaatatgta
     7981 tctttcactt tgttatactc tttttttatg tattccaaaa catctggtgg tatatttcta
     8041 ctgctctttg aagtcaagcc aagttcccca tcttttattt taaattccat tactttactt
     8101 cccttaaaac catcttttgc aacgttcttt atcagttcat atacaggttg ttctacatct
     8161 tttataattg aacttattac attatctgga gccatatcgt attggtcttt attaatagct
     8221 ataactttac caccattttc tcttgctacc tgaatagctc ttttactatc atcttctgta
     8281 gctgaaaata caacatctct atcagaatct ctcatttcct ttgcaatagt tctagctgat
     8341 tcagggtttt caaatatatt tgaatactta actagtaact cagcatcttt atttgaagtt
     8401 ttaagcccct ttctaaatcc agattcatac ttatctctac tagaaccctc aactcctcct
     8461 ataaaaccga ctttcccagt atcagtcatt ttacctgcaa taagtcctgc taaatatcca
     8521 gtaacattat cttgaaatga tacagaaatt acattagctg gctgaacttt ataagtatag
     8581 tcaactattg caaattgctg tttgggatat ttagtagcag cttctttaat tggctctgca
     8641 aatctggctc caactgctat tataaaatct ggttcatact tatgcagttt ttctatactc
     8701 tctttaaaac ttttagaagt ctttggtttt tcaagtttta tcctaacatc aaaatcttcg
     8761 tcagcccttt ttaaggctga tagtacattg ttattatatg attcgttatt taagttttcg
     8821 gtatctaaca atactccaat agtttttctt acatatgaat catcttctgt gtcatcttct
     8881 ttattcttac ttttactatt tgatttatcc ttttctgctg aagttagcaa acttttagac
     8941 tcactctgtt tcgatttagc actatctata ttagaattat tcttatttac tttatcaaac
     9001 ccaaaatcaa aattacatat agctaatata aaaattgtaa aaactactac aaagcaagcc
     9061 atcaatatcc tttttatttt cacaaaattc actccctgat ttagataggt ctgacctacc
     9121 tctgcatatg tctttctata tatgtctatt ataacatcca attcacctca taactaaact
     9181 cataagtatt cttggatatt atagtagatt atttattgtt cttattaaca tattctaaca
     9241 catctggagg aatattttga gaactatttt gggcaagtcc agtacttcca gacttaactg
     9301 tgttttctat aacctttccg cctttataat ttccttttac aaaactttgt atcagattat
     9361 aagtaggatt ctcaaaatct tttacaatag aacttataac attttctgga gctaactcat
     9421 gttggtcttt attcgttgct ataacttttt tattttttgc ccttactgca tctattacag
     9481 cttttgaatc atcttcagta gtagaaaaaa taatatccac tccattatcc atcatttttt
     9541 tagctataga ttctactgat ttgctatctt taaaaacatc tgcatactca actgaaatat
     9601 cctttatact actattagaa aatttaacac cctctctaaa acctgattca aatttatctc
     9661 tactagaact ctttgttcct cctataaaac caaccttatc gctttcagtc atttttccag
     9721 caattaatcc agctaggtat ccagatttat tatcttcata agaaattgaa gttatattag
     9781 aaggttgctt gtcatactca tagtctataa ttgcaaattg ttgctttgga tatttcttgg
     9841 ctaccatctc caatggtttt gcaaatctag ctcctacagc tataattaaa tcgtaattgt
     9901 cattacataa aatatctata ctattagaaa aagttgagga atcatctgat tccactttgt
     9961 ttgtatctat attaagtttc ttttgagcat tgtttaatgc taataaagct gaattattca
    10021 tagcttcatt atttgttcct tcaatatcta gtatcattga tacagatttt tttgaattac
    10081 ttttatcgct tatatcactt ttattatctt tgctagagct aggattatta cttgggttat
    10141 tagcacaccc aaccaacatt ccagctatca ttgctacagt taacatcaat atcaatattc
    10201 ttttgaaacg cattaaattt cactcctcat acatttacga tatttttata tcctatttac
    10261 ttttacatcc tatttataat ttttttatat acaatttaca agtacatttt gaaatttttt
    10321 taataaatct tactataaaa aagtgtgcaa tagattctta tctatttaca cacttcccta
    10381 caaactaaat ctaatatatc aacacttaat ttcatccaaa ataaatccaa tttaacatct
    10441 caacaattaa tattctatcc taatttaata aaaaaataaa taatgtcatc caaaaatata
    10501 ttatttattt tttaagcata tgataccagt aacaccagga ccagcatgtg cacctataca
    10561 cacaccaatt tggaaaaatc taatttcttt aggtcttaat tcatcttgca taaccttaga
    10621 taattgttct ttttccttaa aatcatcact atatcctata tacacaattt ggtctgataa
    10681 atcatcacca caatttgatt ttatcaattc catcatctta gaaattacat tcttctttcc
    10741 tcttacttga gatatttgcg aaactaatcc atctcttatt tcaagaatcg gttttatatt
    10801 taatatactt cctattgctg ccttagtaga tgatattcta cctccctttt gtaaatattc
    10861 caatgtatct actgaaaata taacaaaagc cttctctttt aattcatcca aaacaggcaa
    10921 tatttcagca acagactttc cttcacttgc aagttctcct gcttttatta caagtaaacc
    10981 agttcccata caaagcaagt tagagtcata tgtatatatt ttaccatcta catcattctt
    11041 tgccattaca gcactttgat acgtacctgt agctgtggca gaccctgcta tataaagtat
    11101 ctctctatcc tcatttgtat atttctcaaa aatttcctta aattgggcat aagtagcttg
    11161 agaagttttt ggatatacat tttcttctct aagcctttta taaaattcat ctccagatat
    11221 atcaattcta tccttgtatt ctctttcctc cagtataact gttaatgaaa ctatatctat
    11281 atcatattta tctaacaaat cttttggcac gtccgataaa ctgtcacaga ttatctttag
    11341 atttttcatt aagtatgctc ctttctcatt taggcttgtt gaaactacct catatttggg
    11401 tatcctattt gtcttaaagc ctcgtacact acaatagcaa ctgaatttga taaatttaat
    11461 gacctagctt tttcaacatc caacataggc actctaatac aagtatccat atttgctttc
    11521 aacaaaggtt ctggtaatcc ctttgtttct ttcccaaata ctataaaaca attttcttca
    11581 tatttcacat ctgaatgtga ctgtttagcc tttgttgtag aaaaaaagta tttactattt
    11641 ggatactttt cttgtaattc ttcaaagcta tcgtaatatt gtatatcagc tatatcccaa
    11701 tagtcaagcc cacatctttt taggtgttta tcatccaatg aaaaacccag tggttttact
    11761 aaatgtaaag ttgaacctgt tgctgcacag gttcttatta tatttcctgt attttgaggt
    11821 atttctggtt ctactaatac tatatttaat gacattttaa tcctccctaa gtatcgcttt
    11881 tatttatcaa attttatttt ataaacagaa taacaataat attatactat tatataaaat
    11941 aatgttaata ataattttta tttcatgtat ataatagttt tttatctaac attttcacaa
    12001 gtattattat atcagaaaat ccctcttgta caacttactt tctaaaagtt atttcgtttc
    12061 attaagttct ttataataat tacaatcttc ttttattggg caacttgcac attctggatt
    12121 tctagcttta cacattcttc ttccatgaaa aattaataca tgatgtgagt gtgaccatct
    12181 ttcttttggt atagcttcca taagtgcaaa ttctgttttt tgaggatttg gctcgtccac
    12241 tattcctatt ctattgctaa ctctaaatac atgagtatct acagctattg ctggatgatt
    12301 aaaagcgtta ctaagtacaa cccctgccgt ttttcttcca acaccaggta attttataag
    12361 cttttctaag ctatcaggta cttcaccatc atataattca catagctgtt cagatgtatc
    12421 ctttattttt tgagacttgc ttttgtaaag accacaactc tttatctctt tactaatttc
    12481 ttcaatgctc aaattagcaa agtctctagc tgtgttgtac tttttaaaca actctgaagt
    12541 aactttattc actcttacat cagtacattg tgctgataaa atagttgcta ttaaaagttc
    12601 aaaggctgtc ccataattta actcacattt agcatctgga tacaattttt ctaactcgtc
    12661 taaaatctta tttacgtctt tttcatttgc tgactcattt ttaattttag acttactttt
    12721 agtttcttta cttgtttttt gtgtagcctt attagaacta ctcttctttg tttttttctc
    12781 cgtttcacct tttttagtac tttttttact tactttgata ctttttttat cttcttcaat
    12841 atccataaaa ttctctatat ctttctcatc aattatctca tcttttttac caatcatttt
    12901 atctattcct ttttcattta tctcttcctt ctcaattata tctttcttat tcattccttt
    12961 tttatttatc tcttccttct caattatatc tttcttattc attccttttt tatttatctc
    13021 ttccttctca attatatctt tcttatctat tcctttttta tttatctctt ccttctcaat
    13081 tatatctttc ttatctattc cttttttatt tatctcttcc ttctcaatta tatttttctt
    13141 atccattcct tttttattta tctcttcttc caaaattaca tctttttcat ttatctcttt
    13201 tagatttaca tttttcataa ttatccccct tcaaaatacc aatttataaa acgctattta
    13261 taaatagttt aatcatatca acaaaaaaat aaacatcaat atataaaaca tacaaaataa
    13321 gaatttaatt tatatatatt tacaaaaaaa gcaaaaacct attaaagttc ttgcttttta
    13381 ttatacataa atacccatta aattctataa taaacttatt cttttgacac agtttgtatc
    13441 ttgctgaaag atattaattt ttcttttacc aatttcttta tttcttcaaa tttaacatct
    13501 gtcaatatct ctatagcctc ttctactctt tcaacagtat atatcttaaa ttttccatct
    13561 cgcactgcat cattaacttc atctgataaa acgagatttc tttgattttt tctaggaatt
    13621 atgacgcctt gctcaccatt taacccttta gttttacata tagaataaaa tccttctatc
    13681 ttttcactta taccaccaac tacttgtata tcaccctttt gatttataga acctgtaaca
    13741 gcaatatttt gtttaattga tactccacta agagatgata atagtacata taattctgca
    13801 gaagatgcac tatctccatc tattccacca taattttgtt caaaacaaac atatgcattt
    13861 agtgacaata gtaaatcctg tgcaaaattc tcagaaagat agccacctaa tataagtact
    13921 cctttgttat gtatagagcc actcatattt acttctcttt ctatatttac tatgccttta
    13981 cttcctgggc tagtggtcgc tgtaatcctt gaagctctac caaaagagta ttctcctgta
    14041 cttaagactg acaaaccatt tatcactcct acttttcttc cctctgtttc tattagagta
    14101 aatccatttt caattgattc atccatatgt gtttccactc tattgattct ttttctcttt
    14161 tcacttattg cttttttaac atcacactgc tctgtatact ccgaatttct aaactctgca
    14221 taagcacttc cttctattac aacttccatt attttattaa acttagttga taatttttct
    14281 aaatcaccag ataatctagt gctaaattta ataacctctt caactgctga tttagtaaaa
    14341 tgccttaaat tattcttatc acattggtat gctataaatc ttgctattcc ttcttcatta
    14401 tcttcatttt tatccatttc actatcaaag tctacaaata ctttaaaata tttttcaaaa
    14461 tcttcatcat atctatataa aatatcatat atatagtggc ttccaatcag tactaattta
    14521 acatccaatg gcatattttc tggtttaatt gctgtttgtg tttccaatat tactttttta
    14581 gtctgcaaag tctttttaag aatttcccaa gacattgtat attttagtaa ttggtctgca
    14641 taaagtacta aataaccacc attagcccta tgaaatgctc ctgataatag ttttgtgaaa
    14701 tcagtcttta tatttccatt tgcataatca tactctgcct ttccaaatag atttgctgga
    14761 cttgggttta tctctactac aacaggtgct gattctttat tctttgagct tccattgtct
    14821 acaaataggt taattttata tttaataaaa tgctctttat catacttatc ttttaactcc
    14881 tcttcatcta aatagaataa atacatatat tctattatat ctttctttat attttccaga
    14941 taagtttgaa tcttatcata attttcatat ttttcacaca atctcttgat atgaggttct
    15001 actacaagtt tagctatctc ttcttcaagc tctaacactg ctttttcagc tatttcttct
    15061 aagtttctaa ttttatatac gacctgtatc gccatatttt ctagttctct tttagctttg
    15121 taaaactctt catcagactc atcactttca tcttcctctt ctttcaaagg gataaaaact
    15181 attcccattt tactctgttt taatttaaat cctttttctt ctccatattt tttaatttgc
    15241 ttcaaaagca tatctttttc tatttcatat tcatccagaa gtttattttt ttctatttca
    15301 aaattttcac tttcaaacgc atttttaaaa tcttctaaaa gactttcaat taatttctcc
    15361 atatctttct taagttctct tccaaacccc ttttctaatt caactataat aggctctcta
    15421 gggttttcaa aattatatat ataacaccaa tctttatgcg tatttttttt ggatgcatac
    15481 tcatttaaaa cctttaaggc ataagtactt ttaccagttc caggttcacc agctatatat
    15541 atgttgtacg ctggattatt tattttcaac cctaattcca tagcttctac agctctttct
    15601 tgacccaata tataatctag tggatttatt tcagatgtat tattatattt caatattccc
    15661 ccacctcccc atatttaact ttaatgatat aatatgactt ttacacaaca agtgttaaag
    15721 taaatacaca ttaaagtata tgaaacagac tcttattatt agtcatcata cttatctaaa
    15781 attatttcca tatttctctt tattatattt cttcctctcc agctaaaagc tctatctcca
    15841 tatttccttt taaattcttt atttgaaata ttttgtatct catcataatt caaataatct
    15901 attatatttt ctttaaattc caaaatatga gttgttggta tttcaacatt atgtggacat
    15961 acttcttgac aaatatcgca tccaaatacc tttttgccat tctttaaaac aactttttct
    16021 ttctctgaca aatctccttt tttttgagtt atatatgata aacatttttt ggggttcata
    16081 tcataatttc ccaatatagc atttcccgga caatatttta cacactttcc acatttaaaa
    16141 caactttttt ctagtggttt atcttcttca aatttataat tatttactat ataccctata
    16201 aatacgtaag aaccatattc atcagttatg atattattat ttattccaaa atatcctatt
    16261 cctgataaat aagctaaata cctgtcaact aatggtccat tatcagcaaa taacttgtat
    16321 ttaaaatctg ttactgtttt agaaatataa tcacaaattt gttgtaaaaa gtcctttact
    16381 acaatgtgat aatcctttcc atgacaatat tttgataaat ttgaattttt attcttttct
    16441 tgtctattta tatgatatgg aaatgcacag actattatag agttagcatc cttcattata
    16501 tgtcttgggt taattctgtt ctcaattatt ggttcttcca taccagttaa ataacctttt
    16561 tgttgtctgt ccttaagtat cttttctaaa ttatcatact tatctacccc tgctactcct
    16621 acacagttta atcctattaa attacaaaat gcttttaatt ccttattatc cataaaatta
    16681 ttccttttta atctaattga ctttttaaat ttatttaatt tttttagtct ttttaacctt
    16741 ttctagctac taactctttt aaatacttaa tctttttagc tattcaattt tcttacctac
    16801 ttaaatcttt tagatacttc gtctttttac ctatttaatt ttttacctac taacgctttt
    16861 agatacttaa tctttttatc tatttagttt tttacctact aactctttta gatacttcat
    16921 cttttttatt tactaactct tttagatact tcgtcttttt catttactta tttaactttt
    16981 ttatctactt aactccttta aataattagt ctttttatct atttaattat tttacctact
    17041 aactcttttg aatacttagt cttttttatt tatttaattt ttttatctac taacgctttt
    17101 agatacttaa tctttttatc tatttaattt ttttatctac taactccttt atatacttag
    17161 cctttttcat ttacttattt aactactaac tcttttagat acttagcctt tttcatttac
    17221 ttatttaact tttttaccta cttaactctt ttagatactt agccttttta atctattcaa
    17281 ccttttttac ccacttaacc ttcttacctt ttaatataaa ctctaaaact tcaaatatca
    17341 ttagttatat tttgacattc tttctatagt taagcaaaaa gattgatttt ttcttataat
    17401 taatatccca tttacattgc catttattaa atctttactt gacttatatg gatttctttt
    17461 tactctcttt actaaactat acttattaat attatgaagg tcttataata gactaatatg
    17521 aatatgtcca tcatatatca aattttaaaa tttgtaagca aaatacttat cttatattac
    17581 tacatctaaa gtggaaaatt ttacaaaaaa gctctgtaaa aacactttaa ataacaattt
    17641 gcttttaaat catatacaga ttataataaa tactgtataa ggatggtgaa aaatttgatt
    17701 aaaataacaa ttccaggtag acctattagt aaatctaatt ttaagcttca taacataaat
    17761 ggtcaagcat ggatgccttc aaagggaaaa tattctaagt atcttgcata cgaaaacatg
    17821 attgcaggtt ttataaacca acagtatcaa ggggaaactg tcgaagaaaa tctaataaca
    17881 gtcttgaaat tattcttccc aaataagaga atgggggatt tacataatta tccaaaaagt
    17941 atttgtgatg gtattgaaaa aagtggaata ataaaaaatg ataaacaact taagccagta
    18001 cttttatttg attttataga taaagataac cctagggttg aaattgaact ttatccagta
    18061 tctaaatacg atatatccta taatattttt gaaaaataaa gattttatgt gttattcgac
    18121 attatattga tactccagta tttgaaacta tactaataca acaataataa ttgtgttatg
    18181 tctatactca aacaaatgtt tttaaaaagc attaagtaaa tatttaataa gtattaaaca
    18241 aatattaaat ttatataata gctataacta tgtaaattta atatttgtat ataagaccac
    18301 atctaataaa aagaaatcgg agtaaattta atacaccgat ttcaataaat aaaatctatc
    18361 ctagcttttc aacaacagtc ttaagcattt ctcttatagg aagttcatat ggacatctac
    18421 tctcgcaaag cccacagtca atacactcag taggtttaac atccataacc tcatattttt
    18481 ctttagccca ctcttttaat ccatatctag tataataacc ttcacataaa aaactcaatg
    18541 gtatatttat tcctacagca caaggcatac aatattcgca tcttctacag aattttttac
    18601 ctaaagaatt tcttatttct tcaatttcct tattatcttt ttcatctagc acaagatttt
    18661 caagtacagc gacattttgt ctaacctgtt caacactctc catacctggt ataactacat
    18721 ctatgtactc tttagatagt atatatttaa tagccagtgt tgcattgtct aaagcacctc
    18781 cagctaaagg tttcattact attatcccaa ttcctttttc atgagctttt ttaaacactt
    18841 catctgcttg tccttcaact atattataag ggaattgtat agtgtcaaat tttccatctt
    18901 ctatagcttt ttctatagta tttaaattgt gactagttat accaatatgt tttatcttac
    18961 cttgctcctt tgcctctaaa agggcactat aagccatttt atctttaaaa agattatcat
    19021 actcctcttc tttaacatta tggaattgat ataaatctat aaaatcagtt ttaaaattat
    19081 ttaaacttat ttcaacatct ctcttcattg aatcataatc tctggacata cttttagtag
    19141 ctaagaaaaa tttatctctt tttccttcaa tggcaattcc gattgcttcc tcacttattg
    19201 tataccctct tgcactgtca ataaagttta ttccttgttt ttctaactca tttataacta
    19261 agttagtatc atcttgagta atcctttgta ttggaatgcc cccaaagcca actctcttta
    19321 ttttcatgtt agtattgcct aaatttctca tatctttccc cacctaactt ttaataattt
    19381 tttatataat ataaatatgt cacctttaat tatacaacaa attatgagaa tctttgcaat
    19441 gtatttacat actattcata atataattat attttatatc agtctattca taatataatc
    19501 atactttata cccagttaat ttgtatacaa ttttcgttat tcctatccat taatttaaat
    19561 tatcacattt catgattgta tctccaccct aattatgttg agggtggagt atttcacata
    19621 tttttaataa aggtttattt actagatagt atttctttta tcattttaag ctctctcttg
    19681 tgaccttcat catcaaaagg agtttctaag aaaaatggta aatgttttag actaggatgt
    19741 tccataaaat caattaatgc ttttaatcct atctttcctt cccctatagg agcatgtctg
    19801 tcttttttgt ctccaaatgg catcatacta tcatttagat ggacagtttt caatctttct
    19861 atgcctataa ccttatcaaa ttcttccaaa accccatcta aatcattcac tatatcataa
    19921 ccagctgaaa aaatatgaca tgtatcaaga cacacaccta ttttttcttt atgctcaatt
    19981 ttgtctatta tactttttaa ttgctcaaat ttaaaaccta tttcagttcc ctttcccgac
    20041 atagtttcaa gaagtatagt tatattttca tcacccttta tggcattgtt caatcctttt
    20101 actattctat ctatgccaaa atctacacca ccacctacat gacttccagg atgaaaacac
    20161 atgtattcaa ttcctaatga gtccatcctt cttatatcct catctatcac ccttgttgca
    20221 aattcgtata catcatcctt tgtaccaccc agattcatag tatatggtgc atgtgcaagc
    20281 agtggtgcaa aattattttc ttttctaatt tcttgaaatt taattatatc tttttcatca
    20341 aattctttag catttcctcc tcttggattt ctactaaaaa attgaaatgt atttgcacca
    20401 atatatacag ctgtctcagc cgcattagca aatccttttg ctattgaaag atgtgttcct
    20461 aatttaatca tattaatctc cttttatttg tactactact tgtactcaat tttaattttg
    20521 aactactcca ccttaactac atttagccaa catatgaaga tggagattcc tatttgatag
    20581 tactttttca ctaaaaattc gtaagctcta agggatgttc tatcccaact aatctcattc
    20641 ctcttttaac atatttctag cattgattta atttatatta atttttaatt ttaatattta
    20701 cactttaatt atacccattt taatcaattt caaatattat aatcttacat actttgtata
    20761 ttatctaagc acttcttaga tagtgtatac atcttacact atacatatta tttacaaata
    20821 caataggtat aaaaaaacaa aaaaatagag attaaaaatc attgttttac ccataccaaa
    20881 aactattttt tgattattaa gtaaattttg atgttcaatc tctaatatta tatctttaat
    20941 taatccataa ttaattctct aactaggaca ttcctcctca agagtaggaa ctataaccat
    21001 agctatatca tacttagaac cttcaacatt gtgttgtctt ttatttaaaa tttcatctat
    21061 ctttgacttc atagtgtcac taagttcaac taattcatca tgagtaagag aaatatcatg
    21121 ataatcaact atattttcat tttctaaaac atcttcctct gctgccttat aaaaaacttc
    21181 actcatattt tcaaattttg atatgtacaa ttcttctttt tcttccccat tgtctaaaga
    21241 aaaattaagt atcctattcc ctataaccac attttttgca cttggataat agtacttttc
    21301 cacaatcccc gactttattt tttcctcaac taattctaaa ataccaactt tgtataacat
    21361 ttttatatgg tagtttattt ttgcatgagg ttcttccaat aattgagata attgttttgc
    21421 tgacaatggc gatttgtcaa atgcttttag aatatctatt ctacgtggat gcgcaatagc
    21481 ctttatgcac tccaaatctc gtaataccaa tacctctttc attgacccct cctaaaaaat
    21541 ttatttaatt catatttctt tacaatttaa ttatatatta aattatatgt tgttgtcaaa
    21601 aaaatccttt cttaatattt taaaaaatat ttaatttgag tttatttgac aactcctcta
    21661 aaactttaac tcctgcgacg ctgttacctt ttttgtcaag cgcaggtcca taaacaccaa
    21721 tccccatgcg tcccggcaca gaagccatta taccaccccc aactccagat tttgcaggaa
    21781 tgccaacttt aatagcaaat tccccagaag catcatacat tccacaagtc atcataaatg
    21841 tcttgacaat cctagaaacc atttcactca ttaaatgctc accattttct atatctactc
    21901 cataatttgc aagatttatt ccaattctcg ctaaatctac agaatctatc tcaattgagc
    21961 attgtttaaa ataaaggtct agaacatctt ctacattccc tctgataaat ccatcacttt
    22021 tcaataagta agccatagct ctgtttctat ctccagtcat tttttcagac ttatagacat
    22081 catagtttat tcctatatta tcattttttg ctaactttct aaagaaacta agcattcttt
    22141 cttctttttc ttctaaacaa ctcccattta taagggaagt ggttactata gcccctgcat
    22201 ttatcattgg attacagggt ttttttgtgt cattaatttc aagtttcatt attgaattaa
    22261 atgggtcccc acttggctcc attccaacgt ttgaaaaaac gtcttcccaa tcattatcca
    22321 taagtgccat agcaagcaca attggttttg aaatactttg tatagtaaac tttgtgctac
    22381 agtttccagc actataaaga ttattttcgc tatctatgat acaaattcct aaatcatttc
    22441 ttctagcgtt ttttaattct ggtatgtaac tagcgacttg tccataatta gtatacttct
    22501 tattagaact tataatttct tttaataatg tctcatctaa cgtattcata tgcacctctt
    22561 atattatgtt atatattcta ttttagcaca agtttatact ataataaaac aatgttaaaa
    22621 aaaattatac tttaaaatta ttttctacaa gaatcaattc atttttcaaa caatttaaat
    22681 agtttaaata gcttaataat ttaataattt aacaacctag caatttagta atctaataat
    22741 ccagtaattt aacaacctaa taatttagca atctaataat ctaactatct aataatccag
    22801 taacttaaca acctagtaat ttagtaattc aacaatctaa caattcaata atttaacaat
    22861 ttaacaacct agtaatctaa ctatctaata atccagtaac ttaacaacct agtaatttag
    22921 taattcaaca atctaataat tcaataattt aacaacctag taatctaact atctaaataa
    22981 tccagtaact taacaaccta gtaatttagt aattcaacaa cctaataatt caataattta
    23041 acaacctagt aatctaacta tctaaataat ccagtaactt aacaacctag taatttagta
    23101 attcaacaac ctaataattc aataatttaa caacctagta atttagcaat ccagcaatct
    23161 aataatccag taatttaaat ttttataaca ccaaaaattt ctaacccaac accttttact
    23221 cttttactct tttactcttt tactctttta ctcttttact cttttactct tttactcttt
    23281 taaaaaataa tcacataaat taacaaaata ttaaacaaaa aagtaaaatt taaaataaaa
    23341 agcttgcttc atacaaaagt ataaaacaag cctttttata tatcaactca aactttaaaa
    23401 tctgtatcaa tttaattctc cactttacac ttaatatact taacataaac caataacact
    23461 acttgctatg ccatttaatc ttcataaaac aatctataat acacacatta atcaactatt
    23521 ttacttcctc caataaattc cctaagaatt gaaatacctg gtatgtcaga agtatcgtcc
    23581 aattcaacaa aatactgcaa aaactttagt aatctagtag cttcaatatg tgcaggatta
    23641 tcttccttta tctcttccaa caaaggtttc gcaaatcgtt ttaaaactgc caattcatat
    23701 acacaagcag agttaaatat agcatctgcc tcctcttggt acgggaatat attcttcttt
    23761 tctcctcttc taactgatgc ccattgtaaa atagtattat cagcactata tcctctaaaa
    23821 ttatagtctc taacaattct tcttatcatt ctcaaatcag cagtagggat tcgattatgg
    23881 tcatccaaat ttatttgagt aagggcactt atgtatatct taaatttatc ttcatctggt
    23941 atagatgatg ttaaaatagg atttagccca tgtatacctt ctaatataat tggctgattt
    24001 gaatctatct ttatcttttt accagtgtat tctctcttac cttctttaaa attgaatctt
    24061 atatcatcaa tttcttcacc agctaaaagt ttctttaagt ctaagttaaa cttctctaaa
    24121 tctattgcat aaattgattc aaaatcataa tttccatatt catctaaagg agtatgtttt
    24181 ctatctataa aataatcatc taaagaaatt gaaataggat ttaaattatt tactctaaga
    24241 tgtattgata atctgtgagc aaaacttgtt tttccagatg aagatggagc tgctattaaa
    24301 actactctct tattgccatc ctttatcata tccgctattt gggataactt cttttcatgc
    24361 aatgcttcaa atgtccttat tatatctcca tattcattgt tttctattat tttattaaga
    24421 gatactacct tgtcaattcc cataagtctt gaccattctt ctgcttcata atatacactt
    24481 gatagcttag gttgaggtac aaatttcata ggaagagtct tatcttcttc actaggtcct
    24541 aaaattataa cgcctccatt ataaagctta atatcaaatg attttatata accagttgat
    24601 ggcaacatat aaccataaaa atggtctata tagtttctac acttatatac tttagcacta
    24661 tcatactctt tgtatttcaa aagctctgcc ttttcataca tttcattatt ttcaaagata
    24721 gaaatagctt catttcttgt aacttcaatt ttttctataa cataatcgtt gtttactatc
    24781 tctttcatct tgttttttat attttcaaca tctgcttctt taagctttct atctatatga
    24841 acttcacaat atagcccatc tgatagtgaa tgttctattg taactctgct atcccaaaag
    24901 atttctctac atgccattac aaatataaat gacatgactc taaagtaaac tctctctcca
    24961 tcttcattag tagtatctaa aaaatttatc tcacaatcct tcttaactct acaatttagc
    25021 tcttttagtt tattgtctac aactgcaagt gttatataac ccttatgctc atcacaaaat
    25081 tcttttgcta tatctcccaa tctgattcca ggggaatttt cttctaatga atattcctta
    25141 tcttttcctt ctatttttaa gtttattttt ttcacttcaa catctccttc cttttgatga
    25201 atataaattt taattaatga ggcatatcaa caagataaag agaggtattt aaccctctcc
    25261 tacaatatta ttcaatttac ttatagtatc ttcaatgtct tcgtaattta tttctaatac
    25321 taaatattct gcatctaatg ttaagatctc tttaatcccc tcttcagata aaattcctct
    25381 tcctatacca acatgtgtat cattgatacc atcattatca cttaagtgta tcagtcttat
    25441 tttatccttt aagttcttta cataagcatc ctcatcaatt aaacagtgtc cagtatcgta
    25501 acaaaaacaa atcttagaat ttttaatcct cttaaatata tattcaaaat cataagatat
    25561 atttccaata tttgaaaaat ccccaccttt tgaataagta ttctctatac atacatatga
    25621 atttgtattt aatttatcta aaaaatccac actatttcct aaatatttgt ctctattctt
    25681 tctaactcta tttgtcataa catatcccaa atgaaggtta aaatacctta aatcaaatcc
    25741 ttttagttca aattctatct tctcaataaa atttatccat gaatttcgta tatactctat
    25801 gttttcgcaa gtatttaatt ccataggcaa atgtatccct atagataatc ctaacttaga
    25861 aattctctct ttatatttac ataactcact acattcagat atattgtcaa ttccaatctc
    25921 taaatgagtt atttgcttta tactctcaca aatgtttaaa gcctcctcaa tattaaacaa
    25981 taaggacgct ataccaactg ccatacaaat cacctactca aaaataccta tatctgtaaa
    26041 aggaaatact cttaaaataa cttttccttt aatattttct tcatctacta atcccacttc
    26101 tttatatcta ctgtctaaac taacttctct attgtctccc atcgcaaata cttttccctt
    26161 aggaatcacc atatcaatat cgccttcagt ataattatca tgtatatatg aaacttcatt
    26221 taataactta ccatttatat aaaccttaga atccttaatt tttagatggt caccaggaac
    26281 acctataacc ctttttacaa gctctttttt ccttccattc tcttgtaata aatcagtctt
    26341 aaatatcatt atatctccac attttggttc tgataactta tatgtcatcc tgtttgctac
    26401 caaataatca tgtgatttaa gtgttgggta cattgattct ccatttacta atgttggttt
    26461 tataaaacga gttataataa atgcaaaaaa aagtgcagta ataattattt ttatccactc
    26521 tacaatctct tctttaatag tttcattcat tcacaaattc tcctttatag ttatattatc
    26581 tttattgata atgtctctta aacactatct tttagataag tctactaaat aaggtgttgt
    26641 cttttttata tagacaacta ttgtgtatgg tctggtgatt tcagctaatc agcgattgct
    26701 ccaaatttta tatgtttagt caatagttcc tatcttagaa aatggataca atctgactag
    26761 aacctttcct aaaatggtat gctcgtcgac caatccaacc tcatcgtatc tactatctaa
    26821 acttttttct ctattatctc ccattgcaaa taattttcct tctggaacta cgatatcaat
    26881 atctccatca gtacgattat tatgtatata ggaagtctca tctaacaact tatcatttac
    26941 atataccttg gagtcttgta tttttacatg gtcaccagga accccgataa ctctttttac
    27001 taaatctttt ttctttccat tttcctctgt taagtcggtt ttgaatacta ttatatcttt
    27061 gtattttggc tctcctacct tgtacgcaat tctgttaact atcaaataat cacgttcaac
    27121 taatgtagga tacattgatt ctcctttgac tattgttggc actataaaac gagttattgc
    27181 aaatgccaaa acaagagcta tgacaatcac ttttatccat tctacaactt cttttttaac
    27241 tgcttcaccc actaaaatcg tctcctctgt gttaaatatt aagtcaatct acagcaaaat
    27301 aatctaccat aaacctacaa tataaatatt atacttcttt tttctaatag tttaaaatac
    27361 ttttctaaga cctttaggat aatgattctt aattacacca gaagattctt ttccccaacc
    27421 taaaggtatt ccttcaaccg aaaccaaaac ccatcctcta ctatcattag aatttacaac
    27481 atcccctttt aaatatttaa gaattgattc actctgtaca ctgaagtttt caacattttt
    27541 tacatcttca acttttaagt aatgagatag tgcatgagaa ggctcaaatc tattcttctt
    27601 taaaacccct aaatgcaaac catatctcaa tacttttagt tttttcgtat ctggatatat
    27661 ttcaggaagt aggtacaaat tttctcctct taaataaaat ttattttttt tattactata
    27721 aaaatcaacc tttaaaaatt tcttttcaaa ctccctataa tcctttattt ctttatctaa
    27781 attttttatt tttaattctt tgacattaca ttcttcttct tctaattttt ttattttggc
    27841 aacaaaatga ccttctccct ttatcttatg aggccataat ctttccattt caataagttc
    27901 tgcatgtgga tattcactga taaactcatt tataacatgc tcattttcct cccttgaaaa
    27961 agtacaagtt gagtaaatta aaatgccatc cttctttaac attttatatc catctcttat
    28021 aatctctttt tggatggatt gacattctac tacctttgca taactccaat ctgatatggc
    28081 aacctcatcc ttcctaaaca taccttgccc agaacaaggt gcatctataa taaccttatc
    28141 aaaataccca ttaaacgtct ttcttaagct attagaatcg gtgtttgtta taatacaatt
    28201 tctcgctcca aatcgctcta aattttcccc tagagcctta attctactag cattaatttc
    28261 attagaaacc aataaacctg tatcgtttag cttagatagt atatatgtag attttccacc
    28321 aggagcagca cacatatcga gaactttttc accctcttta atatctgcct ttggtattac
    28381 actcattgca gaaggctctt gcaaatagta tgctccagcc tcatgcagtg gatttctacc
    28441 aggtctgtca attttatcat cataataaaa accttcttct gcccatggta tttgattcaa
    28501 atcaaacaac tccaaatcaa ataatttttt cttgtcaatt tttaaagtat ttattctaag
    28561 cccagtagtc ttactttcct cataactttt tataaaatca tcatattcat cttttagtat
    28621 ttcttcaata tcttctaaaa attcctttgg taattctgtc aaatctataa tcccccttca
    28681 atctgcttta cttgtgtgtc atttattata ctttaattat ttatgtcttt cctttaaatt
    28741 ttatttcttc ttctttttcc taagattacc gaaaaacact tttttagagt gattcatata
    28801 tttctcaagt ttatcaagat ttatcttcca caaaattatt acataaacta taagtaatat
    28861 aatagcccat ctatattcta tcatcaatat gaaatcaaaa actccatgta ataaaattgg
    28921 aattaacacc gccataaaca aatactcacg tcttttattt ttatttccct caaatttata
    28981 cttagaaata tagtagccca tagtaattgc aaacattaca tgagctggta tagagataac
    29041 tgccctatta ataccaactt gcagtgctag atttctactt tcagaaaaaa tataaaccat
    29101 attttcaatt gttgcaaatc ctaaagctaa aaatacagag taaataatac catctaactt
    29161 ttcagtaaag tgtttttctt taattagggc aggaatcagt atcagagctt ttacaccttc
    29221 ttctacaagt cctgcaacta caaatgatac atatattagt tctgaatacc cttcaaatac
    29281 attaaacttc atcagtaaat tttctaaaag tataattata acacttagaa aagcacctat
    29341 taaaaaaaat ttaattagat ggattagggg ttctctacaa tatctatctt tagaccttat
    29401 ccaaaacatc cctatcaata taggaattat tgctaataaa aataaatcta gcttcataga
    29461 ttattttcct ttcaaaattt ttgatttatt tatgatttat atcatctact tatttttaac
    29521 ttattcgtgt tttaaataca tactaaaata tacattaaat tattttaaat tatttaaata
    29581 caaatatact caaatactca aaaaaattta aatttatatc tatattattt aacaaatttc
    29641 aagctatatt catatcttga aatataaggg aactaaattc atttaaagaa agggtgattt
    29701 gatgcaagat ggtttcttaa cagtaagcat aattgatgct actaacaata gacctattca
    29761 aaatgcagta gtaaacatat attcaatgtc taatggtagc caaagctcat caactttata
    29821 ccaaaaccta agaagtaatg aatctggtca agtgactggt ttagtattac cagcaccaga
    29881 tgtagattac tctctacaac catctgatgt tagaccttat agccagtata tagtggaagc
    29941 catagcagat ggatatgaga cagttgtcat agaaggaaca cagttgttag ctacaattga
    30001 agcaagacaa ggagttccca tgtctcctag aacaagaagt aagcgttctt tctcaagaca
    30061 aagtgagttg atttttgata taggagaaca tactctatat ggtacttatc ctcctaagat
    30121 tccagaaagt aacttaaaac cattaccacc tcctactggt ttcgttgtat tagataaccc
    30181 cgtagtgcct gaatttatag tagttcatga tggtttgcca gaagattcat cagctccaaa
    30241 ctattggatt ccatttaaag agtacattaa aaacatagcg tcatctgaaa tatattcaac
    30301 ttggcctgaa cagacaatct atgcaaatgt aatagctata atatcattta ctttaaatag
    30361 agtctttact gaatggtata gaaataaagg atataacttt acaataactt caacaacagc
    30421 atacgaccat aagtttataa ataatagaaa cttatttgaa ccaattaacg tagttgtaga
    30481 tgctattttt aatactttta taaaaagacc tcctacatca agacagccac tattagcaca
    30541 atattgtgat ggacaaaaat cccaatgtcc tgaccaaatg actcaatggg gcagtaaaga
    30601 cttaggtgac caaggatatg actatgagag tatactaaga tatttctatg gagatgagat
    30661 agtatttgaa agagcaccta tagtaagtgg tgtaccagtg tcattccctg gtactacact
    30721 acaagttggt tcttctgggc agtatgttag aaccatacaa aatcagttaa atgctatatc
    30781 taattcttat ccagctgtac caaaagttat agaagatggt atctatggaa cagatacaga
    30841 aaacgctgtt aagatattcc aaggaatctt tggtttacct caaagcggag tagtagattt
    30901 taaaacatgg tatgaaatat ccagagttta tgtagctaca actagaatag cttctttaaa
    30961 tcctttaatt taaagcttga tttagaacat aaactcaaat tattgaataa aattattaat
    31021 ttaaaataaa agtggctgtt atgaactaat atataccata agtattactt ataagtattg
    31081 tagtcttaac agccattttt tagttataac ttgttttttt agaatcttaa aactttaaat
    31141 gggggatttt aagcttctta aaaacagtgc ttagcaactt agatttcttt aaaatattat
    31201 atttattttt aagtttaata aaatgctatt ctaacgaaaa acctactttt tatttaaaat
    31261 ctcacttatt atttaaaaca ctatccatca cttagaatat tacttcttat ataaattatt
    31321 atttattata aatacaaata atattaattt aaaaagttat tcctaattta aataatacta
    31381 ctatcttcat cagtgaacct aaactgcaac tttagactta ttagctaaag ccaatattag
    31441 acctaaagct atagatgcaa atgcagaagc aattactcta tactcagcag ggaataaaaa
    31501 tacaattgta tgacatggta tccagaaaaa tggtatagtt tttaaaacac taaaaccaag
    31561 gtaagcattc cagtccacaa tatttaccac atctttaagt tttaccttaa tcccctttcc
    31621 aacctcatat tttttatcta taaaacagtc cataacctta tggaataaaa atacagttgg
    31681 tgacattagt atattcatag ttgcactctt aaatagagca tttgcaaaag ctattccctc
    31741 aaatggaagc ataccatcca cttgtgcttg acctacacct gatgagtaga ttgacattcc
    31801 atatgtggta actacaccta ttattcccca aactattgct ctataaaata ctcctactgg
    31861 tattttccaa tccttataca aaattctaac accaagtaaa tctcccattg tagctaatac
    31921 agcaaacttt aaaaaacctc caatatatgg atgtgcacct gttgcttcca taaatacttc
    31981 tctactactt ggtattgcta agacagcaat aatacaaata agaactccac tccatataaa
    32041 atcgcctttt ttcatattta taaatactat ttaatatata aatagttctc ctttctatta
    32101 aagatttatt taatttacaa tctatctaca attatttatt aatcacttat tattagatat
    32161 tcattttcca tatttaaatt tattctatcc ctttatctat ttattttctt actactctat
    32221 ccaccattat ctatatattt attttacgat tactctttca ccctaaccct ttaactatct
    32281 attttttctt actattctta tccacatcat ccatatattt attttacgat tactctttca
    32341 ctctaaccct ttaactatct attttttctg atattcttat ccaccatcat ccatatattt
    32401 attttacgat tactctttca ccctaaccct ttaactatct atttttttct ggtattctta
    32461 tctactatta tctgtatatt tattttatga ttactctttt aacctacccc ctatacttta
    32521 tagtaatatg tttttaacct ttttcattat tcccaatatg ctttataact acaaatactc
    32581 tcccccctta aaaataataa taaaattaat caataaattt ataatcatat tattactcta
    32641 tgtattaagc aattactatg ccaataaact ttgatttttt taaacaaatc ttaaaaatcc
    32701 taaaatgcta attttcttta acttttaatg aatctaatag ataacattta attttcgtta
    32761 tatttttcag aatatttcta ttattatttt taagaaaaaa aaataatgtt aacatttctt
    32821 aacatacatt aaaaaatgtt aacattataa attgggttta atacttttac tttgactcga
    32881 tttactttta ttctacttct tactttattt ttaacttaga ctttattttt ttattactta
    32941 tttgagtctt atttatttta ttctcactcc atatgttcaa ttacatttgt ctaaattctt
    33001 tgatactttt attacataat tttaacaagc ttaagtgtta tttgggtagt gcagttgata
    33061 attatttaaa taatcatcaa ctgcatgttt atatcattac agtagcttaa tgtatttaat
    33121 catacttact taggattttg aaatatctta gagattatat ttaacattag tcaattgctg
    33181 cataattgta agcaattgaa atttgagacc catcttcatt ttctatagtg ataggcttca
    33241 taatttttaa tccttcagtt tttatctttt ctaattcttg ttccattttc ttaattgttt
    33301 cctcaaggtc tttttgactt acatctttga ctgcattagg gtctttagca tagtctttta
    33361 attcttgaat atgtttagca tactcttcat aagtataatg ctcaacacca tacttttcca
    33421 attcagtagg gtcagttatt cctgatttta ttagagattc aaatttaggt aaagcaatat
    33481 tagatgaatc atttccattt gcaaatactg ctccagttcc tcctagtacc attgcagata
    33541 ccatcattgc acttataagg gattttttta ttttcataac caatcttcct ttcttagaga
    33601 tttatttggg tagtgtagtt taaaatgact attgcaatag ttatcaacta cactcttaga
    33661 ctatcacagt agtcatgttt atgtcaagcc tttttaaaaa acttttcaat aaaaaatact
    33721 atctcaaagc aaaattaatt attttgagat agtattttaa ttctaaatct tttatattac
    33781 taagatttat ggttattcta tccatgttct atattgttct ttattaactg ctaacaaatt
    33841 cccttttctt aatatgacaa agcgattatc attatagcca cttcgctcaa ttgaaacttc
    33901 tcctgaatca gtataaagta catccttact attactagat ttatttacat acgtttcttc
    33961 actattctca gaattagtta ctttgctcat agatgattct tcatgctctc taaattcaat
    34021 tacagatact tttgccgtac taaaatgagt ctctccatca cttttagctt tactaacaag
    34081 aatatcagat tctactttgg gagaatataa atcagttgat ttactactta ttgaaaatcc
    34141 aattttatca ctttgttcat tttttgttaa accaaaagat tcaacctcat taatctcttt
    34201 attttctgtt gcaaaaacaa gacctgtccc acaaactaat acagttgaaa ctaaaaaagt
    34261 aacataagtt gattttttaa atttcataat attaactatt ctcttttcta tttttgtctt
    34321 tccaaatcca ctacatattg tcattgaaaa tggattattc tcctctgcta gacttattag
    34381 tgtggttgca taatcagccc tatattcttc accaagtata ctcataacct tttcatcaca
    34441 actaaattct atgtctttat ttagtaaaaa aaacatcacc caaactaagg gattaaacca
    34501 atggacaaca agagctacaa gcataaataa ctttaacaga ttatcaaagc tattaatatg
    34561 aaccatttca tgagttaaaa caaagtctaa agtttgatta ttagcataat tcatttgctt
    34621 tggcaaaata attcgtggtt ttaaaatacc agatgtaagt ggtgtcctag taaattcaga
    34681 tatgcaaaca ataaatcttc taaaacggct attcttacac atccattttt taatgaactc
    34741 atgatttttt atatgaattg aagttttaaa aagattaatt attttaaaat atctaaagca
    34801 taaaagtatg ctaaacataa tagtaactat aaaagacaaa atgcccaaca tttccaacat
    34861 cataatattt tcaatattag acattatttc aaattcgtca gaattgtttt tacctgtaga
    34921 gtcactagct ttcttaagag tataattttc tgttgatgta ttacccaatt ccacatcaaa
    34981 agtcgattca attatactat aaatactaaa accagaattt aaacttactg gtattagaag
    35041 tttaaataaa acaatcaacc acaataatac aaataccttt ttaggtaatt ttttaaataa
    35101 acatgctctt ataaaaataa ctattaaaag taatactcca gaagtaaaac tcatttctaa
    35161 caatttaatg aacataaatc ttacctcact ttaatttttc aacaatttgt ttaagttttg
    35221 taatctcatc acttgaaaga ttttctttat taataaaagc tgaaagaaat ttttctgaag
    35281 accctccaaa cattttatta attaaagttt ctgtttcctg tttttgtact tcctctttgc
    35341 ttattaaagc tttacaaaca aaattcggct caattctctc tattgcacct tttgacacta
    35401 atttttttat aacggtatac gtagtgtttc tattccatcc tgtttcttct tttaaaattt
    35461 ttactaactc tccagccttg ctttctcctc tctcccatag aacttccatt actttaagtt
    35521 ctgaatcaaa taattttatc tccatataaa atcgctcctc aaatatagtt atcacaatag
    35581 tcattactat aataaaaaac tatcatagta gtctatacta tgtcaatcat ttatttttta
    35641 ataataaaaa ctgtcttttt aaaaaattct tataaaagac agttttatca atgttatact
    35701 ttaatgttta attaaagcca tattttattt aaatatataa ctatctatct tagtacatag
    35761 tataagattt ttctttatct aatagtatat cctctacatc tgggttttct tttactaatt
    35821 gtctataaaa attacaatta gtaacaccca catatggaat tagccatagt agacctattc
    35881 caagagtagc tattgaaagt atataccatc ctataaagct aaagtttagt acaaataatt
    35941 taagtacatt tcctttcatc atatctaaac tagcacccat tgatttaaat acacccatat
    36001 tatccttgtc ttcacaaatt aagaaagtag cttgagaaac atataatagc aatatataaa
    36061 ctggtaacat aagaacaagt attataagaa ctagagttat tccagtagaa ataatagtcc
    36121 ctattccaac attatcactt gataaatatt caattgctcc actagtaagt aaaactccaa
    36181 atgataaaaa cgctcctata attgtgatta taaaagaata aatatacaca ataattgatt
    36241 caaataaata aaaaccaaaa cttcttaaat aaattctccc acttacaaag atatctccta
    36301 tcttgacttt ttcatcttta gtaattttca agaacataca tgtaaatcct gctgaaacgc
    36361 aaaaagctat tacagccact acaatagtag tcaatggttc aaaaggtaat cctacaattg
    36421 atattaatac tatcaatgca atatatactg ccatagaaat tgcacatatc ccccagtttc
    36481 cagataactg agctttagat actcgtttta attcttgcct tgataccata aaaaacaccc
    36541 ctttcatttt ataaaaaaac ttttaaaaat ataaataatc ttttaacgta taatattaat
    36601 atatcatatt ttcgactata taagatacaa ttattcacca tgttttatca agatatgcca
    36661 taattgtatt actagtttta tatagtcaac attaccaaat acatcaaata aatttgatta
    36721 attattcaac aagtaataaa ataatttc
//
LOCUS       DAMJVK010000035        35385 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.035, whole genome shotgun sequence.
ACCESSION   DAMJVK010000035 DAMJVK010000000
VERSION     DAMJVK010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 35385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35385
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.035"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            101..433
                     /locus_tag="PC414_001790"
     CDS             101..433
                     /locus_tag="PC414_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HDF2464187.1"
                     /translation="MKVAYIFSSTNTHKILDKMIIPQLEQGTHGVDVLGMFFFMDNTL
                     FLSKGNPVGERLSKLHEKTDMIIMACDQCAIERGIENNLVDGATIGCFPNLYACLGSA
                     GVDQVITL"
     gene            595..780
                     /locus_tag="PC414_001791"
     CDS             595..780
                     /locus_tag="PC414_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464188.1"
                     /translation="MSEKYADKLEIKLYQAGKDFSYIKKYGIITKGTLIINQKKKYDR
                     LNKDTIERAIVEAINNN"
     gene            807..1892
                     /locus_tag="PC414_001792"
     CDS             807..1892
                     /locus_tag="PC414_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDF2464189.1"
                     /translation="MNILEIVLNGVKDTIVASIVTLNGASGWLMISFILAGVLHNVLT
                     PRRFQKQLGNKKFSSLIKSTISGMFLPICSCGVIPLGISMYYSGAYLGPVLAFMTSTP
                     IINPIAVVLCLGLLGPKVTIIYLIAGFLVPFLVGFLGNCFGKEELSLHNDSEEEVIEL
                     EEEKRSFMGNMIDGLKWSFGELALTISKYVVMGMVFAGFITTIFPNSIIQKYLGNPGL
                     LSLGSIAILACVMYVCAVGHIPFIAALVASGASPGIAITFLIAGAATNLPELISMFKL
                     IGKRTVIIYSVTLTLSSIIIGYITNLLLVNNNSVNLDATNSSIQLANKLMLNIPDSFE
                     YVCTILIILFAFKSIIPKMKEVIGNHA"
     gene            1885..2343
                     /locus_tag="PC414_001793"
     CDS             1885..2343
                     /locus_tag="PC414_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464190.1"
                     /translation="MLKKIVIISCIVVVLIILSKIVDDNIKEDASIPNVNKETLEYFR
                     KNYKEDIITCAEEDLNNDGKKDLVVIYKKSNNSNEMVVVVSDKNSHYITKPIPAPIEN
                     QTITFKNIDDKAPIEVIVSGSKNGNVGYAIYRVEGKKFVDLFGEDMDKCC"
     gene            2378..3469
                     /locus_tag="PC414_001794"
     CDS             2378..3469
                     /locus_tag="PC414_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2464191.1"
                     /translation="MKLSKKIQALILFGVMGTSILTGCSSKPKEKEEAKTGKDDYTIT
                     VGYYNCDHMTAGPVAESAGIYKDLGLNVKTVGNGKVPEAMAAGKMDAGYIGTKGLVGA
                     IPKGSPITIAANNHTGGSEYLIVSKDIKEPKDLIGKKIATDMSDFLWTSDYGPETGLP
                     TDPSKYEVVNMDSDKDKYLALKTGKIQAFTSCDPWGSVAENDGAGKIIASTQYKEKAN
                     GKEYNCCSFSLNKNFIKEHPDLAKKLVLAHTKAIEYIYTNPAEAAKIFAKYYNVEEEV
                     ALRTIYKKTVGEGRTLTWKVTGEEYKNNLQMYKDLKALDDIPKYEDTIDTSLLDSCGA
                     DDFDKFIKEKVNPEFPEGMSYEDWKAKVL"
     gene            3474..3698
                     /locus_tag="PC414_001795"
     CDS             3474..3698
                     /locus_tag="PC414_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464192.1"
                     /translation="MENMKNIIILFIIILGSIFIIFFNFEDKEEIKGKLRIGVSDDTS
                     GFVINYMVNRDYFENIKVSDIMDIFTINDC"
     gene            3708..4271
                     /locus_tag="PC414_001796"
     CDS             3708..4271
                     /locus_tag="PC414_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464193.1"
                     /translation="MQWALSSEEIDIAIICKDAAKQYVNVNSGFEIVGTVVQNSDIFL
                     LSDNNPEKIGVIQNRDYQSELVKKYYKNVEVAPLLGTALAYGLESNLVDGVVIDCIKS
                     IGLEGKRKSTTKLGDYDTYVLVVNKEFKKSKAYSDFITLYNKSVDALKNKATFKKVLK
                     EYRNIRNIDMEEISNWKLKFLKIKAID"
     gene            4232..4861
                     /locus_tag="PC414_001797"
     CDS             4232..4861
                     /locus_tag="PC414_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2464194.1"
                     /translation="MEIKISKDKSDRLTKKKTDGSMDILYKIILLVIIFLVWHFAAKD
                     IGSSLLLPMPVDVIKGFFFCVTDAETVTNLFITLQRVLKGFMYALLFGLPIGFIMGFS
                     KTFERVLSPVVDSVRQVPIMAWVPLTIVWFGIGDGPTIFLIAFSGVFPIILNTIQGVR
                     AISKDYYNAARSMGASPIVIFTNVIVPASLPDILTGSRIAISTGWMSVI"
     gene            4901..5038
                     /locus_tag="PC414_001798"
     CDS             4901..5038
                     /locus_tag="PC414_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2464195.1"
                     /translation="MVQAQTLMHTDVLIGLMIFAALIGFIIDRVLKFINKILCRWRFA
                     D"
     gene            5038..5790
                     /locus_tag="PC414_001799"
     CDS             5038..5790
                     /locus_tag="PC414_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2464196.1"
                     /translation="MKLSVRDINKTFVNNRVHTKVLEDISMDIDDGQFVCLLGPSGCG
                     KTTLLTIIGGFQKSERGDVFINDKRVKKPGIDRAFIFQNYALFPWKTIRGNVLFPMKQ
                     QKIPKEKREEMLEELLVMSDLKGKEKLFPHQLSGGMKQRVAMIRALACNPEVLLMDEP
                     LGAVDFQMRQNLQEELERIWIKKKITALMVTHDVDEAVYMSDRVIVMSRDKGRIIDDI
                     NIDIPRPRIRGSQKYEEYKNKLTDTLSKCYEV"
     gene            5803..6288
                     /locus_tag="PC414_001800"
     CDS             5803..6288
                     /locus_tag="PC414_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464197.1"
                     /translation="MSQIEKYNSLDNTQQKIRISIMDMIIDKGSSVTLQEVTEYVSKK
                     LNIEKEYIERTLQYFIYKNIMVVDGNSINFIYPVSALPTNHKVTLRDNRSFSAMCAID
                     AIGTSCTFNQDIKINSICSATGKEIEVIIKNEKIEYVNNPNLRVLHINLDKHLNWAAS
                     C"
     gene            6295..6444
                     /locus_tag="PC414_001801"
     CDS             6295..6444
                     /locus_tag="PC414_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464198.1"
                     /translation="MNFFWTKSDFDAWTNEAGLSDDEDIYCLDINEAIVESYKIFKLK
                     QKVLS"
     gene            complement(6580..7401)
                     /gene="yidA"
                     /locus_tag="PC414_001802"
     CDS             complement(6580..7401)
                     /gene="yidA"
                     /locus_tag="PC414_001802"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HDF2464199.1"
                     /translation="MYKLIALDIDGTILNTQKRITPEVFESIQEAKRAGAKVVITTGR
                     PLPGVKELLNQLNLTDEGDYVICFNGAIIQEVKSEKIIHDVEMSLDDFDFIYNNVCKK
                     YKTKIHINTMTNLITPNETPGKYTLHEAKLNNIEVKYIQKDKIDESIKICKIMIVDEP
                     ERLEEIIQQLPKNLFNKYTIVRSAPFYLEFLGKTTNKGTALKTLCTNLNIPIENAIAV
                     GDEENDQHMIKYAGLGVAMGNARNSIKEIADYVTDTNNENGVAKVINKYILNKAI"
     gene            7894..9588
                     /locus_tag="PC414_001803"
     CDS             7894..9588
                     /locus_tag="PC414_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HDF2464200.1"
                     /translation="MKFSEFKYERPNYNSMKKEFLSCVEDINNSRNYKEQQKNIHKIN
                     LLRNKIETLSNIASIRYSTDTFNKFYKEEKNYWDEYMPLYEELNSYFYNAIVNSKFKY
                     DLIKEFGEQFFTIVEYSLKSFSKEIISELQEENKLCSEYTRLLASAEIMFDGKIRNLS
                     GMGKFMYSKNRKTRELANKAYYNFFEENETKFDDIFDKLVKLRDKMSKKLGFEDFVEL
                     GYIRMMRSDYREYMIKNVRKQVLKYVVPMANELYEKQAKRIGLEYLSYIDEGVEFLTG
                     NASLKGDSRYIIKNGKRMYSELSKETNEFFDFMLENELMDLETKKGKGAGGYCTYIPD
                     YKSPFIFSNFNQTADDIDVLTHEAGHAFQLYMSRWIDMPEINFPTLDSCEIHSMSMEF
                     ITWPWMDLFFKEDTDKYKFTHLSSSIKFIPYGVIVDEFQHYIYKNPNVDKSKRKEIWR
                     FLEKKYLPHRKYGDNSFLERGCWWFKQGHIFKNPFYYIDYVLAQICALQFWKKMIQDR
                     DAGWKDYINICKVGGTKSFLDIVSMGNLYSPFDDGCIESIIGDVKSWFDEINDSKL"
     regulatory      9920..10030
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            10134..10505
                     /locus_tag="PC414_001804"
     CDS             10134..10505
                     /locus_tag="PC414_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdX family protein"
                     /protein_id="HDF2464201.1"
                     /translation="MIIITNNPKVKEEVQGREVLFKDTTYIGILEASRDLIHEGYELL
                     SHPLYGSVKPNETPYRTVILKKGNRLDINSLTLIEEAIITASKFQNNKKTPKWTESVQ
                     DDFRVIDYDIFYNTIQRMQYE"
     gene            10600..11547
                     /gene="trxB"
                     /locus_tag="PC414_001805"
     CDS             10600..11547
                     /gene="trxB"
                     /locus_tag="PC414_001805"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDF2464202.1"
                     /translation="MENVYDLVIIGSGPAGLAAGLYGARAKLKTLILEKDKTGGQIVI
                     THEIANYPGSVPNATGPSLIARMVEQCKEFGAEMLRDNIVDTELDGDIKVLKGEKAEY
                     RAKAVIIGTGATPRKIGCPGEKELTGKGVSYCATCDADFFEDFEVFVVGGGDSALEEA
                     MYLTKFARKVTIVHRRQGFRCAKSVEEKAKANPKIEFLLDTVIEEIKGDGILESVVFK
                     NKVTGETHEYFADEEDGTMGVFVFVGLDAQTDLFKGKVDMDEKGYIITDEDMRTNIPG
                     VFAAGDCRSKTLRQVVTATNDGAIASIVAEKYIDEKFGN"
     gene            11651..11968
                     /locus_tag="PC414_001806"
     CDS             11651..11968
                     /locus_tag="PC414_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HDF2464203.1"
                     /translation="MLDLDKATFEEEVLNAEGFVFVDFWSEGCEPCKALMPDVHKLAE
                     TYGDKIKFCKMDTTKARRLAIKQKVLGLPTMAIYKDGEKVDEVTKDDATVPNIENMIK
                     KYL"
     gene            12237..13523
                     /locus_tag="PC414_001807"
     CDS             12237..13523
                     /locus_tag="PC414_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006439082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HDF2464204.1"
                     /translation="MRLELGKIFIKDIQFGDVTEVKDGVLFINKQEMLQEIGGDEHIK
                     SIDIELARPGESVRITPVKDVIEPRVKVEGNGGIFPGIMSKVDTVGEGKTHALKGVAV
                     VTTGKIVGFQEGIIDMTGEGAKYTPFSKLNNVVVIAEPIDGLKQYAHEKAVRMIGFKA
                     AMYLGEVARNLTPDEVSVYETKPLLESIKEYPELPKVGYVYMLQTQGLLHDTYVYGVD
                     AKQIVPTLLYPTELMDGAIVSGNCVSACDKNPSYVHINNGVVEDLYARHGKDINFVGV
                     IITNENVYLADKERSSNWTSKLCKYLGLDAVIVSQEGFGNPDTDLIMNCKKIEMQGVK
                     TVIVTDEYAGRDGGSQSLADADVRADAVVTGGNANQVVVLPKLDKVIGHLEVVDVIAG
                     GSDGSLRADGTIEVEIQAITGATNETGFGHLTAKGY"
     gene            13635..14108
                     /locus_tag="PC414_001808"
     CDS             13635..14108
                     /locus_tag="PC414_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:13767..13769,aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HDF2464205.1"
                     /translation="MSLLSNKKVLIIGDRDGIPGPAIEECVKTVEGAEVVFSSTECFV
                     UTAAGAMDLENQNRVKDAADKFGAENVVILLGAAEAEAAGLAAETVTAGDPTFAGPLA
                     GVALGLSVYHVVEEPIKSLFDESVYEDQISMMEMVLEVEEIEEEMSGIREEFCKF"
     gene            14140..15450
                     /gene="grdB"
                     /locus_tag="PC414_001809"
     CDS             14140..15450
                     /gene="grdB"
                     /locus_tag="PC414_001809"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:15187..15189,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HDF2464206.1"
                     /translation="MGKLKAVHYINQFFAGIGGEEKADTKPHVAETLPPISLQLDKLL
                     GEDIEIVGTVVCGDSYFNENIDSASEEVLSMVKGFEPQLFIAGPAFNAGRYGVAAGTI
                     TKVVKDALNIPALTGMYVENPGADMFKKDVYVVETSDSAAGMRKALPKIAKLAVKLAN
                     GEEIGTPKDEGYIARGIRVNYFHEDRGSKRAVDMLVKKIKGEPFETEYPMPNFDRVDP
                     SKAVKDLSKCKIALVTSGGIVPKGNPDRIESSSASKYGTYSIAGVMDLTEETYETAHG
                     GYDPVYANLDADRVLPVDVLRDLEKEGVIGKLHETFYTTVGNGTSVANSKKYASEIGA
                     ALVADGVDAVILTSTUGTCTRCGATMVKEIEKTGLPVVHMCTVVPISLTVGANRIVPT
                     IAIPHPLGNPALDPTEEKALRRGLVEKALNALTTEVDGQTVFEK"
     gene            15706..17238
                     /locus_tag="PC414_001810"
     CDS             15706..17238
                     /locus_tag="PC414_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014520415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III family protein"
                     /protein_id="HDF2464207.1"
                     /translation="MTYPVLKGAGYVLIHTPDMIVQNGSTCTVERATNPDSEFLKEVS
                     NHIRSYEDVVNYMPNQVYIGNRRPEELRDLPMPWCEQKIEGTRNGKFGEIMPQDEFIA
                     LMQISDAFDLVKLSQEFIDEVKPKIENNYPEIAPFVGKLKGDDIEEGKELVATHIAEG
                     LYHDGKFVGYVKRAHDVDVNLNAHTMFENLVVKASGVLSAIQMLRHSKIDPAEIDYVI
                     ECSEEACGDINQRGGGNFAKSIAEIAGLQNATGSDTRGFCAAPTHALIQAAALVKAGI
                     HKNVMVVAGGASAKLGMNAKDHVKKGLPVLEDVVGGFAVLVSENDGVNPVIRTDLTGK
                     HTVGTGSSPQAVMTALITSGLDRANLKITDVDVYSVEMQNPDITKPAGAGDVPEANYK
                     MIGALAVKRGDLEKKELKDFVSNKGLPGWAPTQGHIPSGAPYIGFLIDDLTTGNRNRA
                     MIVGKGSLFLGRMTNLFDGVSFIAERNTGVTEETSGISKDEIKKIIAESMKKLALDML
                     EE"
     gene            17249..18394
                     /locus_tag="PC414_001811"
     CDS             17249..18394
                     /locus_tag="PC414_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine reductase"
                     /protein_id="HDF2464208.1"
                     /translation="MSKKVIADVFLEVANAIESGEFGKKVKIGVTTLGSEHGVENMVN
                     GAQLAKSNLFDIVLIGPKVETDLEVVEVNDEKEMHAKMEELLDSGYIDACVTMHYNFP
                     IGVSTVGRVITPAKGKEMILATTTGTSATNRIEAMVRNAIYGIATAKSMGNKCPKVGI
                     LNVDGARQVEKCLKELKDNGYDMEFADSIRADGGCVMRGNDLLVGAPDVMVTDTLSGN
                     IFMKVFSSYTTGGDYEAQGFGYGPGVGEDYDRKVLIVSRASGSPVVANALKYAYDVVK
                     GDISNVARNEFAKVKKAKFDDIISSLTKKEVKAEKVEVKMPDKEIVTRQIAGVDIMDL
                     EDAVSELWKNGIYAESGMGCTGPIVLVNEAKGDLAVETLVKAGYTAK"
     gene            18704..19783
                     /locus_tag="PC414_001812"
     CDS             18704..19783
                     /locus_tag="PC414_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF2464209.1"
                     /translation="MSRVKNVVELLETKGVDALYLTKKTNVNYISGFPDEEAYAVICK
                     DGNFLVTDSRYMELAEKVCKDFEIINWHNFDRSVAKAVKSVCDKVGIKKLGFERTNIV
                     FDKYEELKNLIEKDNGELIPTENIVETLRYVKDKDEIKNTRKACEIADKALEELIPHI
                     KAGVSEIELATKLEYFMKMNGAQNIGFETILISGAKTSLLHGKPSDKIIEKGDFVLID
                     YGAMYNGYISDTTRTFIVGGASEKQLEIYNLVKEAQNVGVENMKAGVHATIPDAEIRK
                     VVKKYEDYYYQGIGHGVGRDVHEEPFIGNYGDKIIEEGCIITMEPGIYFPGWGGVRIE
                     DTVLITKNGPERLTKFPKDLMILDK"
     gene            19896..20495
                     /locus_tag="PC414_001813"
     CDS             19896..20495
                     /locus_tag="PC414_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464210.1"
                     /translation="MYKKHKFIKNIKKAWYKIKLIDRCLIIVMAILMFQSIYNLFVNE
                     VNSQDTTTIDVVVRTTSAAIFGYFLSANFIKRPLRKYTNTDVSSNLFIDNNLSPKGQE
                     SNQNNIMNVKNTIGFTSEADNYEKKIPINNNEGFEEGETSELQIIIATVICIVALTVL
                     FVVRNFTTTTSASLGTISQMRDFVSGCVGFLLGCPSKPD"
     gene            complement(20681..21553)
                     /locus_tag="PC414_001814"
     CDS             complement(20681..21553)
                     /locus_tag="PC414_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2464211.1"
                     /translation="MNINYLYYFQTVCKYKNMTKAAESIHISQPSITLAIKELEKELG
                     FELFYRIGNKIELTPEGKIFLDKSKHFIKQFEDFQCDALDLGKKRKASLKIGIPTVLG
                     TFLLSKILPRFNVIYPDIELKIFEVPTFVGAKMIEESTLDFCIGIIDSDIYDDIDSKT
                     IYKTELYLVTNPKNELAKHPIISNYMLKNVPFVILSEGSYHYKIITKRLEKAKPNIIL
                     HSNQLSTIRYLLENDLASTILYKEIFQNTENLCSIPLERAITANIGVLWRRNQYISHS
                     MKLFIEYMASIHIN"
     gene            21970..23373
                     /locus_tag="PC414_001815"
     CDS             21970..23373
                     /locus_tag="PC414_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464212.1"
                     /translation="MKGSDLVKFELMDFLMNPFVLMFAAVITGILFGKIKFGKFNFGV
                     SGALFTGLFIGWLAYSLGNLIIAKGETAAGYKAATVMMGNGIISSDFFDFFLIIFVAA
                     VGLLAAKDMKAVLKKYGARFVILGVLITFIGGSMTYAMTLLSSDKGSSAYEVSGVYTG
                     ALTSSPGLAAALETAGKHAEDVSKEFEKASIKDKKEILKVVDPEGKLDVNTTTSLTQE
                     QIDKYIAYAEAGVGIGHAVAYPFGVLIVILGVNFLPKLFRMDLKEERRKYEKEMKEAR
                     DSVSGKTIPEVPFNIITFFLTCLAGYLVGGIHVFMGPLGYFTLGATGGSLIVSLVLGY
                     IGKIGVVNFRMEEKVLNILKQIGLVFFLAIVGLRYGGKVVDSIMTSGMHLALVAIAVG
                     VTAMMIGFLVGKYVFKLNWILLSGAVCGGMTSTPGLGAAVDALDSDDPAAGYGATYPF
                     ALLTKVILVIVLHKLPM"
     gene            23400..24944
                     /locus_tag="PC414_001816"
     CDS             23400..24944
                     /locus_tag="PC414_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HDF2464213.1"
                     /translation="MNNEGAFEELSGRLRNKELCDLIMNADEAAKIIEDGMVIGVSGF
                     TPSGYPKAVPLAVSERAKSGEKIKLTVYSGASLGPEVDGAWSEAGIIERRLPYQTNSI
                     LRNNINKGVVDYIDMHLSHSTQFLNYGTIPKVDVAIVEALAITEEGNIIPTSGIGNSP
                     SFIKSADKVIVEINLAKPMEMEGMADIYITENPPNRKPIEINHPKDRIGTTYIPCGLD
                     KIAGVVITNMQDKTRPLGVVDEASKKISNNIIAFLREEVKAGRLSKNLLPLQSGVGSV
                     ANAVLYGLCESEFENLTCYTEVVQDSMLDLIRMGKVTMASTTSVSPSPEGLIKFEKDI
                     DFFKDKIILRPQEISNNPEIARRIGVIAMNTAIEVDIYGNVNSTHIMGSKMMNGIGGS
                     GDFARNGAITIFSTESIAKNGDISSIVPMVSHVDHTEHDVMVIVTEQGYADLRGLAPR
                     ERAIKIIENCAHPDYKEQLRDYLNRACKSGAKQTPHILDEALSWHSKFMSTGTMKKAE
                     TFKSAL"
     gene            24975..26366
                     /locus_tag="PC414_001817"
     CDS             24975..26366
                     /locus_tag="PC414_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HDF2464214.1"
                     /translation="MEKAVENFEDLSKEYINGYIERARKAQREFECYTQEQVDKIVKI
                     VGKVVYYNAEYLAKLAVEETGMGVYEDKVAKNKSKAKVIYNNLKDKKSVGIIDIDRET
                     GITKVAKPVGVVAAITPCTNPIVTPMSNAMFALKGRNAIIITPHHKAIGCSTKTVEMI
                     NEELEKIGAPENLIQILDQQSRENTRNLISSADVVIATGGMGMVKAAYSSGKPALGVG
                     AGNVQCIIDRDVDIKEAVPKIIAGRIFDNGIICSGEQSVIVAEEMFDKIMDEFKNNKG
                     FIVRDKVQKEAFRNAMFVNKSMNKDAVGQSVHTIAKIAGVEIPEDTKIIVIEADGPGE
                     EDIIAKEKMCPVISAYKYKSFEEGVAIAKANLNVEGKGHSVSIHSNTVKNIEYAGENI
                     EVSRFVINQCCATSAGGSFFNGLAPTNTLGCGSWGNNSISENLDYKHLINISRIAYYM
                     PENEVPTDEELWG"
     gene            26559..28055
                     /gene="abfD"
                     /locus_tag="PC414_001818"
     CDS             26559..28055
                     /gene="abfD"
                     /locus_tag="PC414_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyryl-CoA
                     dehydratase/vinylacetyl-CoA-Delta-isomerase"
                     /protein_id="HDF2464215.1"
                     /translation="MALMTGAQYIESLRKLNTKVYMFGEEVKNWVDHPMIRPSINCVA
                     ATYDLAHDPEYADLMTVTSNITGEKINRFGHLHQSVDDLIKKVKMQRLCGQKTASCFQ
                     RCVGMDAFNAVYSTTFECDKAHGTNYHDNFVKYLTYIQENDLVVDGAMTDPKGDRSLS
                     PSAQPDPDMFLHIVERREDGIIVRGAKAHQTGSINSHEHLIMPTISMTEADKDYAVSF
                     AVPSDAEGVFMIYGRQSCDTRKLEEGADVDLGNKEFGGQEALVVFDNVFVPNDRIFLC
                     GEWDFSGMLVERFAGYHRQSYGGCKVGVGDVIIGAAALAADYNGANKASHIKDKLIEM
                     THLNESLYCCGIACSSEGHKTEAGNYQIDLLLANVCKQNVTRFPYEIVRLAEDIAGGL
                     MVTMPSEKDFKSDLKVGTSGMTIGEVCNKYFKASSVASTEERMRILRFLENICLGSSA
                     VGYRTESMHGAGSPQAQRIMISRQGNINQKKELAKKIAGIKKEEALNL"
     gene            28190..28465
                     /locus_tag="PC414_001819"
     CDS             28190..28465
                     /locus_tag="PC414_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464216.1"
                     /translation="MTCGVRFCGGCNPRYERGKALTIIKEHFNGRVDFSIAQENVEYD
                     LLLIIGGCATLCASYQQYKVKNECVKMWDFSHIERVINVIESAICET"
     gene            28489..29796
                     /locus_tag="PC414_001820"
     CDS             28489..29796
                     /locus_tag="PC414_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HDF2464217.1"
                     /translation="MSWQELYQSKLCSATEAVKQIKNGDTVVFAHCVGEPPALVEAMI
                     ENAEQYKDVEIKHMVSLGSGGYTAKGMEAHFRVNPMFVSGNVRKAIENGDGDFTPAFF
                     HEVPKLLREKRLKCDVVLAQVTPPDEHGYCSLGTSVDYTYEAIKTARTVIVQVNDQFP
                     RTYGEVVHVSEFDYIVEKSQPLFELQPAKIGEVEEAIGKNCASLIEDGSTLQLGIGGI
                     PDAVMLFLTDKKDLGIHSEMISDGTLALYEKGVINGKYKNFDKEKMTVTFLMGTKKLY
                     DFANNNPAVEVKPVDYVNHPAIIMKQHKMVSINSAIQVDLMGQVVAEAMGLRQFSGVG
                     GQVDFIRGVSMGEDGKAIIAMPSITTKKDGTVISKIVSIVDEGAPITTSRNDVDYIVT
                     EYGIAELKGKSLRERARNLINIAHPSVREHLAVEFEKRFKEKY"
     gene            29816..30934
                     /locus_tag="PC414_001821"
     CDS             29816..30934
                     /locus_tag="PC414_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyrate dehydrogenase"
                     /protein_id="HDF2464218.1"
                     /translation="MEALRVVPKIFYFDTFKEFNEEFKIGKNDLVITNEFIYEPYMKP
                     LGIDTNLIFQEKFGTGEPSDEMIDSMTKEMKKYNFDRIIAFGGGTIVDICKILALDVP
                     EKSIDLFEGEVSPKKVKELVVVPTTCGTGSEVTNVAIAELKSKHTKKGLAVEETYADY
                     AVLIPETVKGLPYKFFVTSSVDALIHAIESYLSPKASPFTEMYSLQAIKMIMDGYKKI
                     VDKGEEERFNHLRDFVLASNYAGIAFGNAGCAAVHALSYSIGGAFHVAHGEANYQFFT
                     EVFKMYSRKKPNGKIIKCTKILADALECDPNCDVYGELEKFLNKLIAKKALREYGMVE
                     SQIDEFTDSTIANQQRLLANNYVELSREEIREIFANLY"
     gene            31221..32141
                     /locus_tag="PC414_001822"
     CDS             31221..32141
                     /locus_tag="PC414_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HDF2464219.1"
                     /translation="MYKSENFSKKSVLINSFLSKFLKIIGWGLVSLFTLSALVLSTIS
                     IIIGWAVILPIQVCAVFLLIGGVCIFKGKRLGDQISRYKKYCTIINNRNIIPVELIAE
                     NTSKSLNFIIKDVQKMIDKNYFINTYIDKRNNQIVLTNEDFVPPRYEDVAYEVKEEEK
                     TDESNEVDTIINKGMNYLTQIKEANKNIKSESMGDKIVQVEDVTSKIFDVVKHDPSKL
                     TQIQKFMDYYLPTTLKLLNSYHTLEEQGIDRENITTTMESIENTMNTIVVAFENQLDY
                     LFEDEAIDISTDITVLENMLVQEGLTSGKF"
     gene            32256..33413
                     /locus_tag="PC414_001823"
     CDS             32256..33413
                     /locus_tag="PC414_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HDF2464220.1"
                     /translation="MNNLDDIPVMPTLTLDPFGEESNTNDIDNSDLLMKKDEKDPEEE
                     KLSESERKMVKEFAEKIDITNTNMILQYGAGAQKKVASFSETALKSVKTRDLGEVGDM
                     LTNLVTDLKAFSADEKEQSGFLGIFKKANNKISNLKTKYDSAEVNVDKVSKELQKHQV
                     KILKDIAMLDKMYELNLAYSKELTMYIIAGKQKLKDMKEYEMPKLREKARLSGSTEDA
                     QSVNDMVSLCDRFEKKIHDLELTRMVSLQMAPQIRLVQNSNNLMAEKIQSTIVNTIPL
                     WKNQIVLALGISHLNQAMKAQREVSNMTNELLLKNAETLKMGTIETAKESERGIVDID
                     TIKKTNQSLISTIDEVVKIQHDGRIKRQEAEVELSKIENELKSKLLEFTVK"
     gene            complement(33498..34997)
                     /locus_tag="PC414_001824"
     CDS             complement(33498..34997)
                     /locus_tag="PC414_001824"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDF2464221.1"
                     /translation="MAFYFDTPSHTFSEYLLVPGFSSTDCIPANVSLKTPVTKFKKGE
                     EADIYMNIPLTSAIMQSVSDDKMAIALAKEGGISFIYGSQTIENEAAMVARVKSHKAG
                     FVVSDSNIKPDNTLKDILDLKEKTGHSTVAVTEDGTSTGKLLGIVASRDYRISRMDLD
                     TKVSEFMTPMSSIVYANKDVTLKEANNIIWDHKLNSLPVLDDNGNLMYMVFRKDYSSH
                     KENPLELLDSSKRYVVGAGINTRDFAERVPALVEAGADVLCIDSSEGFSEWQKITLDF
                     IREKYGDTVKVGAGNVVDREGFLYLAEAGADFVKVGIGGGSICITREQKGIGRGQATS
                     IIEVAQARDEYFEKTGIYIPICSDGGIVYDHHITLALAMGADFIMLGRYFSRFDESPT
                     NKVNINGSYMKEYWGEGSARARNWQRYDLGGDKKLSFEEGVDSYVPYAGSLKDNVTLS
                     LSKVRSTMCNCGALSIPELQKNAKLTLVSSTSIVEGGAHDVMLKDNRNL"
BASE COUNT        13775 a         3997 c         6537 g        11076 t
ORIGIN      
        1 atatagttaa tatctatttt acagataaat aagaataaac ttataatata attgtatctg
       61 taaaattttg taaaatataa aaagtatagg gggaataaaa atgaaagtag cttatatttt
      121 tagtagtact aatacacata aaattctaga caaaatgata ataccacaac ttgaacaagg
      181 aactcatgga gtagacgttc tagggatgtt tttctttatg gataatacct tatttttatc
      241 taaaggtaat cctgtaggcg agagactaag taaattacat gaaaaaactg atatgataat
      301 aatggcttgt gaccaatgtg ctatagaaag aggtatagag aataatttgg ttgatggagc
      361 tacaattgga tgtttcccaa acctatatgc ttgtttaggc agtgcaggtg tggaccaagt
      421 tataacgtta taaattagta taggatacaa tccatagaaa aatttatttt ctgtggatta
      481 ttttaagtaa aagataaatt gtttaaagta ggggattaac aaatgtctaa aattttagtt
      541 gaatttataa acacttgacc atcttgtgat gaacatggag tgtttataaa aaaaatgtca
      601 gaaaaatatg ctgataaatt ggaaataaaa ttatatcaag cagggaaaga tttttcgtat
      661 ataaaaaaat atgggattat aacaaaagga actttaataa taaatcagaa aaagaaatat
      721 gatagattaa ataaagatac aattgaaagg gcaatagtag aagctataaa caataattaa
      781 aagtttaaaa attaggagta tcaaaaatga atatattaga aattgtttta aatggagtta
      841 aagatactat agtagcttct atagttacac tgaatggagc atcaggatgg cttatgataa
      901 gttttatatt ggcaggtgta cttcacaatg ttttaactcc aagaaggttt caaaaacaac
      961 ttggaaataa gaaattttct tctttaatta aaagtactat ctcaggaatg tttttaccaa
     1021 tatgtagttg tggagtaata ccacttggaa taagtatgta ttattcagga gcatatttag
     1081 gaccagtact tgcttttatg acatcaactc caataataaa tccaatagct gtagttcttt
     1141 gtctaggtct tttaggtcct aaagttacaa tcatttatct aatagctggt tttttagtac
     1201 catttttagt aggattttta ggaaattgtt ttggaaaaga agaattatca cttcataatg
     1261 attcagagga agaagtaata gaattagaag aagaaaaaag aagttttatg ggaaatatga
     1321 ttgatggtct taaatggtct tttggtgaat tagcccttac tattagtaaa tatgtagtaa
     1381 tgggtatggt ttttgcagga tttataacaa ctatttttcc aaatagtata attcaaaaat
     1441 atttaggaaa tccaggtttg ctatctcttg gaagtatagc aatacttgca tgtgttatgt
     1501 atgtatgtgc tgtaggacat ataccgttta tagcagccct tgttgcaagt ggagcatctc
     1561 ctggtatagc gataacattt ctaatagctg gagcagctac aaatctacca gaacttataa
     1621 gtatgtttaa acttattgga aaaagaactg ttattatata ttctgtaact ttgacactgt
     1681 cttcaataat aattggatat ataacaaatt tacttttagt gaataataac tcagtaaatt
     1741 tagatgcaac aaattcttca atacagttgg caaataaact aatgttaaat atacctgata
     1801 gttttgagta tgtatgtact attttaatta ttttatttgc atttaaatca ataataccaa
     1861 agatgaaaga ggtcatagga aatcatgctt aaaaagatag ttataatatc ctgtatagta
     1921 gttgtgttaa tcattttatc aaaaatagtt gatgataaca tcaaagaaga tgcttctatt
     1981 ccaaatgtga ataaagaaac attagaatat tttagaaaaa attataaaga agacataata
     2041 acttgtgctg aagaagactt aaataatgat ggaaaaaaag atttagtagt tatatataaa
     2101 aaaagcaata attcaaatga aatggtagtc gtagtttctg ataaaaatag ccactatata
     2161 acaaagccaa taccagcacc tatagagaat caaacgataa cctttaaaaa tattgatgat
     2221 aaagcaccaa tagaagtaat tgtatcaggg tctaaaaatg gtaatgtagg atatgctatt
     2281 tacagagtag agggtaaaaa atttgttgat ttatttggcg aagatatgga caaatgttgc
     2341 taaaaataaa aataatacat aaaggtggga aatttatatg aaattatcta agaagattca
     2401 agcattaatc ttatttggtg taatgggtac atctatatta acaggatgta gtagtaaacc
     2461 aaaagaaaaa gaagaagcaa aaactggaaa agatgattat acaataacag taggttatta
     2521 taattgtgac catatgactg cgggaccagt agcagaatcg gcaggaatat ataaagattt
     2581 aggtcttaat gtaaaaactg ttggaaatgg taaagttcct gaagctatgg cagcaggaaa
     2641 aatggatgca ggttacatag gaactaaggg gttagttgga gcaataccta agggttcgcc
     2701 aataacaata gcagctaaca accatactgg tggttctgaa tatttaatag tttcaaaaga
     2761 tataaaagaa cctaaagatt taataggtaa aaaaattgca acagatatga gtgatttctt
     2821 atggacaagt gattatggtc cagaaacagg tctaccaact gacccatcaa aatatgaagt
     2881 tgtaaatatg gattcagaca aagataaata tttagctctt aaaactggga aaattcaagc
     2941 ttttacatct tgtgaccctt ggggttctgt agctgaaaat gacggtgcag gaaaaataat
     3001 agcatctact caatataaag aaaaagctaa tggcaaagaa tataactgtt gttcattctc
     3061 attaaacaaa aactttataa aagaacatcc agatttagca aaaaaattgg ttctagcaca
     3121 tacaaaagca atagaatata tatatacaaa tccagctgaa gcagctaaaa tatttgcaaa
     3181 atattataat gtagaagaag aagttgcact tagaactatc tataagaaga ctgttggaga
     3241 aggtagaact cttacttgga aagttacagg tgaagaatat aaaaataatt tacaaatgta
     3301 taaagattta aaggctttag atgatatacc taaatatgaa gatacgattg atacaagctt
     3361 attggactct tgtggagctg atgattttga caaatttata aaagaaaaag ttaacccaga
     3421 gtttcctgaa ggtatgagtt atgaagactg gaaagcaaag gtattataaa taaatggaga
     3481 atatgaaaaa tataataata ctttttatca ttattttggg aagtatattt ataatatttt
     3541 ttaattttga agataaagaa gaaataaaag gaaaattaag aataggggta tctgatgata
     3601 cctctggttt tgtaataaat tatatggtaa atagagacta ctttgaaaat ataaaagtaa
     3661 gtgatataat ggatattttt acaattaatg attgctgagc aagtacaatg cagtgggctc
     3721 tgagttcaga ggaaatagat atagcaatca tatgtaaaga tgctgcaaaa caatatgtaa
     3781 atgtaaatag tggatttgaa attgttggta ctgtagttca aaattctgat atatttctat
     3841 taagtgataa taatcctgaa aagataggag tcattcaaaa tagagattac cagtcagaat
     3901 tggttaaaaa atactacaag aatgtggaag tagctccctt attagggact gcactagcat
     3961 atggattaga aagtaattta gtagatggtg ttgtaataga ttgcataaag tcaattgggt
     4021 tagagggtaa aagaaaatct acaactaaat taggagatta tgatacctat gtattagttg
     4081 taaataaaga gtttaaaaaa agtaaagcat atagtgattt tataacactt tacaataaaa
     4141 gtgttgatgc tttaaagaat aaagctacat ttaagaaagt attaaaagag tacaggaata
     4201 taagaaatat agatatggag gaaataagca attggaaatt aaaatttcta aagataaaag
     4261 cgatagatta actaagaaaa aaactgatgg tagtatggat attttatata aaataatact
     4321 attagttatt atatttttag tctggcattt tgcagcaaaa gatataggga gttctttgtt
     4381 acttccaatg ccagtagatg taataaaagg tttctttttc tgtgtgacag atgcagagac
     4441 agtcactaat ctttttataa cattgcagag agtacttaaa ggttttatgt atgcattatt
     4501 gtttgggctt ccaataggat ttattatggg attttctaaa acttttgaaa gggttttatc
     4561 tccagtagta gattctgtaa ggcaagttcc aataatggca tgggttcctc ttactatagt
     4621 atggtttggt ataggagatg gtccaacaat atttttaata gctttttcag gggttttccc
     4681 tataatttta aatactattc aaggagtaag agcaatatca aaggattatt ataatgcagc
     4741 tagaagtatg ggagcatcgc caattgtgat atttacaaat gtaattgttc ccgcatctct
     4801 tccagatata ctaactggtt caaggattgc tataagtaca ggatggatgt ctgttatctg
     4861 agccgagttt atagctacga gtgccggtct cggttactca atggtacaag ctcaaacatt
     4921 aatgcataca gatgtattaa ttgggcttat gatatttgca gctttaatag gatttataat
     4981 agatagggta ttaaagttta taaataaaat cctttgtaga tggaggtttg cagattaatg
     5041 aagttaagtg taagagatat aaataagaca tttgtaaata atagagtaca tacaaaggtt
     5101 ttagaagata taagtatgga tatagatgat ggacaatttg tctgtctttt aggaccatca
     5161 ggttgtggaa aaacaacttt acttactata ataggtgggt ttcaaaaatc agaacgtgga
     5221 gatgttttta taaatgataa aagagttaaa aaaccgggca tagatagagc ttttatattt
     5281 caaaattatg ctttatttcc ttggaaaact ataagaggta atgttctatt tcctatgaaa
     5341 cagcagaaaa taccaaaaga aaaaagagaa gaaatgctag aagaactttt ggttatgtca
     5401 gacttgaaag gaaaagaaaa actgtttccg catcaattat ctggaggaat gaaacaaaga
     5461 gtggccatga taagagcttt agcatgtaat ccagaggtat tactaatgga tgaaccatta
     5521 ggagctgtag attttcaaat gagacaaaat ttacaggaag agctagaacg aatatggata
     5581 aagaagaaaa taactgcatt aatggtaact catgatgttg atgaagctgt atatatgagt
     5641 gatagagtta tagtaatgtc gagagataaa ggaagaatta ttgatgatat aaatatagat
     5701 attccaagac ctagaattag aggaagccaa aaatatgaag agtacaaaaa caaattaact
     5761 gatactttat caaagtgtta tgaagtttaa agaggagtag acatgagtca aatagaaaaa
     5821 tacaatagtt tggataatac acaacagaaa ataagaatct ctattatgga tatgataata
     5881 gataaaggtt ctagtgttac acttcaagaa gtgactgaat atgtaagtaa aaagttgaat
     5941 atagaaaaag aatatataga aaggacacta caatatttta tttataaaaa tataatggtt
     6001 gttgatggga atagtataaa ttttatatat cctgtatcag cattgccaac aaatcataaa
     6061 gtaactttga gagataatag aagtttcagt gcaatgtgtg ctatagatgc tataggaacc
     6121 tcttgcacat tcaaccaaga tataaaaata aactctattt gtagtgctac aggtaaggaa
     6181 atagaagtaa ttataaaaaa tgaaaagatt gagtatgtga ataatccaaa cttaagagtg
     6241 cttcatataa atcttgataa gcatctaaat tgggctgcta gttgctgaaa tataatgaat
     6301 ttcttctgga cgaagagtga ttttgatgct tggacaaacg aagcgggatt atctgatgat
     6361 gaagatatat attgtttaga tattaatgag gcaatagtag aatcatataa aatatttaaa
     6421 ctcaaacaaa aggtactatc atagatatac atatatgaaa aactaaaaat gacctttaga
     6481 taaaaatgat aggtaatatc catactatat aaattagtat agagtaggga tgtgaaaagt
     6541 atttaaatct aaaggtatat ttttagtttt tttagatgat tagatagcct tattaagtat
     6601 atatttattt ataacctttg caactccatt ttcattatta gtatcagtaa cataatctgc
     6661 aatttccttt atactattac gtgcattccc catagccaca ccaagacctg catactttat
     6721 catatgttgg tcattttctt catcgccaac agcaatagca ttttctatag gtatatttag
     6781 attcgtacat aatgttttaa gagcagttcc tttattagta gttttaccta aaaattctag
     6841 ataaaaagga gcagacctta ctatagtata tttattgaat aaatttttag gtagttgctg
     6901 tatgatttct tccaatcttt ctggttcatc aactatcata attttacaga ttttaataga
     6961 ttcatcaatt ttatcttttt gtatatattt gacttctata ttatttaatt tcgcttcatg
     7021 aagtgtatat ttgccaggcg tttcatttgg tgtaattaaa tttgtcattg tatttatgtg
     7081 tattttagtc ttatattttt tacaaacgtt attatatata aaatcaaaat catctaaact
     7141 catttctaca tcatgtatga ttttctcact tttaacttct tgaataattg ctccattaaa
     7201 gcatataaca taatcacctt catctgttaa atttagttga tttagaagtt cttttacacc
     7261 aggaagaggt ctgcctgttg ttataactac ttttgcccca gccctttttg cctcctgaat
     7321 ggactcaaag acttcaggag taatcctttt ttgtgtattt aatattgttc catcaatgtc
     7381 tagtgcgatt aatttgtaca tatcaacctc caataaatta tatactcaaa tttcctgtga
     7441 aaacgttgac acagctagtg ttgaacaaag tttcagtggg aaatatggtt tgttttgtat
     7501 actattatat caagagataa gtaatttaga aagtttaata atattaattt tttgataaaa
     7561 atattactta tgtgagatga gaatgtaaaa actttgtaat caataattcc aaaattctta
     7621 attacaaata atggtatcgt ttgcattgtt aatataaata aatgtatatt atatagttag
     7681 aagtttgaat aaatagactg atggtaattt gttatattag ttagttttgt gttataaaat
     7741 attgtaattc cattaatcat atgttattat tcaacatgat tataatataa agaatatttg
     7801 atatatataa taatttataa aagagtatta catagtacat ataaatctat atttataaat
     7861 acaataaagt aaagttgtaa aggagtgctt taaatgaagt tttcagagtt taagtatgaa
     7921 agacccaatt ataatagtat gaaaaaagag tttttgagtt gtgtagaaga tataaataat
     7981 tctcgtaatt ataaagagca acaaaaaaat atacataaga ttaatttatt aagaaataaa
     8041 atagaaacgt tgtcaaatat agcttcgata agatatagta cagacacatt taataaattc
     8101 tacaaagaag aaaaaaatta ttgggatgaa tatatgcctt tgtatgaaga attaaattca
     8161 tatttttata atgccattgt aaattcaaaa tttaaatatg atttgataaa agagtttgga
     8221 gagcaatttt tcacaatagt agagtattct ttaaaaagtt tttccaaaga aataataagt
     8281 gaattgcagg aagaaaataa attgtgttca gagtatacta gacttttagc ttcagctgag
     8341 ataatgtttg atgggaaaat aagaaattta tctggtatgg ggaaattcat gtattctaag
     8401 aatagaaaaa caagagaatt agcaaacaaa gcatattata atttctttga agaaaatgaa
     8461 actaaatttg atgatatatt tgataaattg gttaaactta gagataagat gtctaaaaaa
     8521 ttaggttttg aagattttgt agagcttggg tacattagaa tgatgagaag tgactacaga
     8581 gaatatatga taaaaaatgt tagaaaacaa gtactaaaat atgttgttcc aatggcaaat
     8641 gagctttatg aaaaacaagc taaaagaatt ggtctagaat atttaagtta catagatgaa
     8701 ggcgtggaat ttttaacagg aaatgcaagt ttaaagggag attcaagata cataattaaa
     8761 aatggtaaaa ggatgtattc tgaactgtca aaagaaacaa atgaattttt tgattttatg
     8821 ttagaaaatg aacttatgga tttagaaacg aagaaaggaa aaggagctgg agggtattgt
     8881 acttatatac cagattataa gtcaccattc attttttcta attttaatca aactgctgat
     8941 gatatagatg tattaacaca tgaggcagga catgcatttc agttgtatat gtctagatgg
     9001 attgatatgc cagaaattaa ttttccaacg cttgatagtt gtgaaatcca ttcaatgagt
     9061 atggaattta taacatggcc gtggatggat ttatttttta aagaagatac agataagtac
     9121 aaattcactc acttatcatc ttcaattaaa tttatacctt atggagtaat tgttgatgaa
     9181 tttcaacact atatttacaa aaatcctaat gtagataagt caaaacgaaa agaaatctgg
     9241 agatttttag agaaaaaata cttacctcat agaaaatatg gtgataattc ttttttagag
     9301 agaggttgtt ggtggtttaa acaaggacat atatttaaaa atccgtttta ttatatagat
     9361 tatgtattag ctcaaatttg tgcgttacaa ttttggaaaa aaatgattca agatagagat
     9421 gcaggatgga aagattatat aaatatctgt aaagttggag gaacaaaatc atttttagat
     9481 attgtgagta tgggaaattt gtattctcca tttgatgatg gctgtattga gtctattata
     9541 ggtgatgtta aatcttggtt tgatgaaata aatgacagca aattataaaa aattatgaga
     9601 attttattgt ataaaagtaa aaaattaaaa aatatataaa aagtgattat ttgttgtgaa
     9661 aatagtcttg tttttaacta aattgataaa ataaactaaa aaagatttgt acttaaaaaa
     9721 aaaagaaaat acaagaaata tgcaatatca acatttggaa aaaaaagtaa aaatgtatac
     9781 aaaatacata taaaaaaact attgcaaatt ttatagaatg tttatatact taagtcaaca
     9841 gagatagaaa aaagctattt atagaaaaca aaggataaat tgcttaattt taaattttct
     9901 gaaaaaaata ataatccgga tgaagagtat gggagactgc tttataaaaa gcgaccgaag
     9961 ggacaagttt aatcttactt acccaaaaga ttagatgaaa ttctcaggta aaggtgactg
    10021 tacttggacg gggctctgaa aatgatttta acagggaaga caaattggga gatttgtttt
    10081 tcctgttttt tatttatcaa gtaataaata aaagtttgaa agagagtaaa aaaatgataa
    10141 taattacgaa taatccaaaa gtaaaagaag aagttcaagg cagagaagtt ttgtttaaag
    10201 acacaactta tattggaatc ttagaagcaa gtagagattt gatacatgaa ggttatgaac
    10261 ttttatctca tccactctat gggagtgtaa agccaaatga aacaccttac aggactgtta
    10321 tactaaagaa aggaaatcgt ttggatataa actctttaac cttaattgaa gaagcaatca
    10381 tcactgcaag taaatttcag aataataaaa agactccaaa atggacagaa agtgttcaag
    10441 atgattttag agtaattgat tatgatatat tttataatac aatacaaaga atgcagtatg
    10501 aataaaaaaa ttgttaaaaa gttattaaaa cagcctaaat attaaatcta atttaatatt
    10561 aaataaaatg agaaatttaa ttggggagga taagtcaaaa tggaaaatgt gtatgactta
    10621 gtaataattg gttcaggacc agcagggctt gcagcaggtc tttatggagc aagagcaaag
    10681 ttaaaaactc taatcctaga aaaagacaag actggtggac aaattgtaat aacacatgaa
    10741 attgcaaatt atccagggtc agttccaaat gcaacaggac caagtttaat agctagaatg
    10801 gttgaacagt gtaaagagtt tggagcagaa atgctaagag acaacattgt tgatactgag
    10861 ctagatggag atataaaagt tttaaaaggg gaaaaggcag aatatagagc aaaagcagta
    10921 ataataggaa caggagcaac accaagaaag attggttgtc ctggagaaaa agaacttact
    10981 ggtaaagggg tttcatattg tgcaacttgt gatgcagatt tctttgaaga ttttgaagta
    11041 tttgttgtag gtggaggaga cagtgcatta gaagaagcaa tgtatttaac taaatttgct
    11101 agaaaagtaa caattgtaca tagaagacaa ggatttagat gtgctaagag tgttgaagaa
    11161 aaagcaaaag ctaatccaaa gatagagttt ttattagata cagtaattga agaaatcaaa
    11221 ggtgatggaa tattagaatc agttgtattt aaaaataaag ttactggaga aactcatgaa
    11281 tactttgcag atgaagaaga tggaacaatg ggagtatttg tgttcgttgg attagatgct
    11341 caaactgatt tattcaaagg taaagttgat atggatgaaa aaggttacat aataactgat
    11401 gaagatatga gaactaacat accaggcgta tttgcagcag gagattgtag atctaagact
    11461 ctaagacagg ttgtaactgc aactaatgat ggagcaattg catctatagt agctgagaaa
    11521 tacattgatg aaaaatttgg gaactagaat ctgtaaatta agaaatagtg tctgtaaatt
    11581 taatgaataa tatttaaaat taaaattaat atttaaaata aaaataaaaa tttcaagggg
    11641 gaataagaaa atgttagatt tagataaggc tacatttgaa gaagaagttt taaatgcaga
    11701 aggttttgta tttgttgatt tttggagtga aggttgtgag ccttgtaaag ctttaatgcc
    11761 agatgttcat aaattagctg agacttatgg ggataaaata aaattctgta agatggatac
    11821 aacaaaagct agaagattag ctataaaaca aaaagtatta ggtcttccaa ctatggctat
    11881 atacaaagat ggagaaaaag tagacgaagt tactaaggat gatgctacag ttcctaatat
    11941 agaaaacatg attaaaaaat atctttaata tgttgataaa ttattaacat taaaagaaaa
    12001 aatctatttt tatctgaaga taagaagaaa aaggttttta tgtccaactt tgttaaaaaa
    12061 ataattttaa ataaaataat ttttttacaa taaatatttt ttagtaataa aataaagatt
    12121 taaggcataa aaacacaata atatatcttt aaaaaaataa gataaattgt aaaatgatat
    12181 aattttgaga aaaactcaaa tgcatataca caaattaaat aacaggaggt gcaagtatgc
    12241 gtcttgaatt agggaaaatc tttataaaag atattcaatt tggtgatgta acagaagtaa
    12301 aagatggagt attattcatc aataagcaag agatgctaca agaaattggt ggagatgagc
    12361 atattaaatc tatagatata gaattagctc gtccaggtga aagcgtaaga ataacacctg
    12421 taaaagatgt tattgagcca agagtaaaag ttgaaggtaa tgggggaatc ttccctggta
    12481 tcatgtctaa agttgacact gtaggagaag gaaaaactca tgctttaaaa ggtgtagctg
    12541 ttgtaactac tggaaaaata gtaggtttcc aagaaggtat aatagacatg actggagaag
    12601 gagctaagta cactccattt tctaaattaa ataatgtagt agtaatagct gaacctattg
    12661 atggtttaaa acaatacgct catgaaaaag ctgttagaat gataggattc aaagcagcaa
    12721 tgtacttagg agaagtagca agaaatctta ctccagatga agtttcagta tatgaaacta
    12781 aacctcttct tgaatcaata aaagaatatc cagagcttcc aaaagtagga tatgtttaca
    12841 tgcttcagac tcaaggttta ttacatgata cttatgtata tggagtagat gcaaaacaaa
    12901 tagtaccaac tttattatat ccaacagaat taatggacgg agcaattgta agtggtaact
    12961 gtgtttctgc atgtgacaaa aacccaagtt atgttcatat aaacaatggg gttgttgaag
    13021 acttatatgc aagacatggt aaagatataa actttgtagg tgtaattata actaatgaaa
    13081 atgtttatct agcagataaa gaaagatctt caaactggac ttctaaatta tgtaaatatt
    13141 taggattaga tgcagttata gtttcacaag aaggttttgg aaatcctgat actgacctta
    13201 taatgaactg taagaagata gaaatgcaag gtgttaaaac agttatagtt acagatgaat
    13261 atgctggacg tgatggaggt tctcaatcac ttgcagatgc agatgtaaga gctgatgctg
    13321 ttgtaacagg tggaaatgcc aaccaagttg tagttcttcc taagttagat aaagttattg
    13381 gacatttaga agtagtagat gtaatagctg gtggttctga tggaagttta agagctgatg
    13441 gaactattga agttgaaatc caagctataa caggtgcaac taatgaaaca ggatttggtc
    13501 atttaactgc taaaggatat taagaagtat tttattaaac gattgagtaa tgttaataaa
    13561 acatattata gataattata ataagtgtta tagatatata aaaatatatt agaaatataa
    13621 taaggaggat tattatgagt ttacttagta ataaaaaggt tcttataata ggtgaccgtg
    13681 atggtatacc aggacctgcg atagaagaat gtgtaaaaac agtagaagga gcagaggttg
    13741 ttttctcatc tacagaatgc tttgtctgaa cagctgctgg ggctatggac ttagaaaatc
    13801 aaaacagagt taaagatgct gctgataaat tcggagctga aaatgttgtg attttactag
    13861 gtgctgctga agccgaagct gcaggtcttg cagccgaaac agtaactgct ggagatccaa
    13921 ctttcgctgg accacttgct ggagttgcct taggattaag tgtttaccac gttgttgagg
    13981 aaccaataaa atcattattt gatgaaagtg tatatgagga ccaaataagt atgatggaaa
    14041 tggttttaga agttgaagaa atagaagaag aaatgtctgg tataagagaa gaattttgta
    14101 aattttaatg cctaaaagca aaaggggtga aactaagaga tgggaaaact aaaagcagta
    14161 cattatatta atcagttctt tgctggtata ggtggagaag aaaaagctga tactaagcca
    14221 catgtggcag aaactttacc tccaataagt ttacaacttg ataaattatt aggtgaagat
    14281 attgaaatag ttggaacagt agtttgtgga gatagctatt tcaatgaaaa tatagatagt
    14341 gcaagtgaag aagttttatc aatggtaaaa ggttttgaac cacaactttt catagctggt
    14401 cctgcattta atgctggtag atatggggtt gctgctggaa caataactaa agttgtaaaa
    14461 gatgctttga atataccagc tttaacaggt atgtatgttg aaaatccagg agcagatatg
    14521 ttcaaaaagg atgtttatgt agtagaaact tctgattctg cagcaggtat gagaaaggca
    14581 cttcctaaga tagctaaact tgcagtaaaa cttgctaatg gagaagaaat tggaactcct
    14641 aaagatgaag gatacatagc tagaggaata agagttaact acttccatga agacagaggt
    14701 tctaagagag ctgttgacat gttagttaag aaaataaaag gtgaaccatt tgaaactgaa
    14761 tacccaatgc caaactttga tagagttgac ccaagtaaag ctgtaaaaga tttatcaaaa
    14821 tgtaaaatag ctttagttac ttctggtggt atagttccaa aaggaaatcc agatagaata
    14881 gagtcttctt cagcttctaa atatggaact tattctatag caggagttat ggatttaaca
    14941 gaagagactt atgaaactgc acatggtgga tatgacccag tgtatgcaaa tttagatgca
    15001 gatagagtat tacctgttga tgttcttaga gatttagaaa aagaaggtgt aattggaaaa
    15061 ttacatgaga ctttctatac aactgtaggt aatggtacat ctgttgcaaa ctctaaaaaa
    15121 tatgcttctg aaattggagc agcattagtg gctgatggtg tagatgcagt aatattaact
    15181 tctacatgag gtacttgtac tcgttgcggt gcaacgatgg taaaagaaat agaaaaaact
    15241 ggacttccag tagttcatat gtgtacagta gtacctattt cattaacagt aggggctaat
    15301 agaatagttc caactatagc aatacctcat ccacttggaa atccagcgct tgaccctaca
    15361 gaagaaaaag ccctaagaag aggccttgta gaaaaagcat taaatgcttt aactacagaa
    15421 gtagatggtc aaacagtatt tgaaaaataa aaataacaca cacatatatt atatagataa
    15481 aaatataaac acacactaaa aataaaaaat aactatacac aaaaaaataa tatagatttc
    15541 atgaatggat aaattggagt tattcattca tgaaatctta aaaaatgcca aaatacataa
    15601 acaataatag aatcaaatat acaataaaat acaatgatta gagcttttaa taagtttaat
    15661 aagattaaaa aataagtgtt cataaaattt atggaggtgc acaaaatgac ttatccagtt
    15721 ttaaaagggg caggctatgt acttattcat actccagata tgatagtgca aaatggaagt
    15781 acttgtacag ttgaaagagc aacaaatcca gattcagaat ttttaaaaga agtaagtaat
    15841 catataagaa gttatgaaga tgtagttaac tacatgccaa atcaagttta tataggaaat
    15901 agaagaccag aagaattaag agatttacca atgccatggt gtgaacaaaa gattgaggga
    15961 actagaaatg gtaagtttgg agaaataatg cctcaagatg agttcatagc tttaatgcaa
    16021 ataagtgatg catttgattt agtaaaatta tcacaagaat ttattgatga agtaaagcca
    16081 aaaatcgaaa ataactaccc tgaaattgca ccatttgtag gtaaattaaa gggtgatgat
    16141 atagaagaag gtaaagaatt agtagcaaca cacatagctg aaggattata ccatgatgga
    16201 aaatttgttg gttatgtaaa aagagcacat gatgtagatg ttaacttaaa tgcccataca
    16261 atgtttgaaa atctagtagt aaaagcatct ggagttttat ctgctattca aatgctaaga
    16321 catagcaaaa tagaccctgc tgaaatagat tatgttattg agtgttcaga agaagcttgt
    16381 ggagatataa accaaagagg tggaggaaac tttgctaaat caatagctga gatagcagga
    16441 cttcaaaatg caactggttc tgacacaaga ggattttgtg cagcaccaac tcatgcatta
    16501 attcaagcag cagctttagt aaaagctggt atacataaaa atgtaatggt agtagcaggt
    16561 ggagcaagtg ctaaacttgg aatgaatgct aaagaccatg ttaaaaaagg tcttcctgta
    16621 cttgaagatg ttgttggagg atttgcagta cttgtatctg aaaatgatgg tgtaaatcca
    16681 gttataagaa ctgatttaac aggtaagcat actgttggaa ctggttcttc accacaagca
    16741 gttatgacag cacttataac ttcaggtctt gatagagcta atttaaaaat aacagatgtt
    16801 gatgtgtatt cagttgaaat gcaaaaccca gatataacta agccagcagg agcaggagat
    16861 gttccagaag caaactacaa aatgataggg gcacttgctg ttaaacgtgg agacttagaa
    16921 aagaaagaat tgaaagattt tgtttctaac aaaggattac caggatgggc accaactcaa
    16981 ggacatatac catcaggagc tccttatata ggatttttaa tagatgattt aactacagga
    17041 aatagaaata gagctatgat agttggaaaa ggtagtttat tcttaggaag aatgactaac
    17101 ttatttgatg gagtatcttt tatagctgaa agaaatacag gtgttactga agagacttca
    17161 ggaatatcta aggatgaaat taagaagata atagctgaat ctatgaaaaa actagcatta
    17221 gatatgttag aagaataggg gtgaggatat gtcaaaaaaa gtaatcgcag atgtattctt
    17281 agaagtagct aatgcaatag aaagcggcga atttggcaaa aaagtaaaaa ttggtgttac
    17341 tacacttgga agtgaacatg gtgtagaaaa catggttaat ggtgcacaat tagcaaaatc
    17401 taatttattt gatattgtgc ttattggacc aaaagtggaa actgaccttg aagtagttga
    17461 agttaatgat gaaaaagaaa tgcatgcaaa aatggaagaa ttattagatt caggatatat
    17521 tgatgcttgt gttactatgc attataattt tccaataggt gtatctacag taggtagagt
    17581 aataactcca gcaaaaggaa aagaaatgat acttgcaaca acaacaggaa caagtgctac
    17641 taatagaata gaggcaatgg taagaaatgc catttatggt attgctacag ctaaatcaat
    17701 gggtaacaaa tgcccaaaag ttggaatact taatgttgat ggagcaagac aagttgagaa
    17761 gtgcttaaaa gaattaaaag ataatggata tgacatggag tttgcggatt caattagagc
    17821 agatggtgga tgtgtaatga gaggtaatga tttacttgta ggtgcacctg atgttatggt
    17881 tactgataca ttatctggaa atatatttat gaaagtattt tcttcatata ctacaggtgg
    17941 agattatgaa gctcaaggct ttggatacgg acctggagtt ggagaggact atgatagaaa
    18001 ggtacttata gtatcaagag catcaggttc tccggtagta gcaaatgctt taaaatacgc
    18061 ttatgatgtt gtaaagggag atataagtaa tgttgcaaga aatgaatttg caaaggttaa
    18121 aaaagcaaaa tttgatgaca ttatatcctc tcttactaaa aaagaagtta aagctgaaaa
    18181 agtagaagtt aaaatgccag ataaagaaat tgttactcgt caaatagctg gtgttgatat
    18241 aatggattta gaagatgcag tatctgagtt atggaaaaac ggaatctatg ctgaaagtgg
    18301 tatgggttgt acaggtccaa tagttttagt aaatgaagct aaaggagact tagcagttga
    18361 aactttagtt aaagctggat atactgcaaa ataagtacat tttttatatg taaataagaa
    18421 catatagtta taattttatt gattataatt atatgttctt tttattttat atcttgtcaa
    18481 tatttatata ttaacacaat ccatttattt gattctctag ctaatttatt atatttattc
    18541 agttaaaatt aagctttcta actaaataaa aactaatgaa gtattaaaat gaaattgata
    18601 aaatatgaaa aaattttagg aataaagtat ctgttagata aaaataagat taattagtga
    18661 tataatataa ttaagataaa atttattatt tggaggcaaa atcatgagca gagtaaaaaa
    18721 tgtagtagaa ttgttggaaa ctaaaggtgt tgatgcacta tatctaacaa agaaaactaa
    18781 tgtaaactat ataagtggtt ttcctgatga agaagcatat gcagtgattt gcaaagatgg
    18841 aaacttttta gttactgata gtagatatat ggagcttgca gaaaaggttt gcaaagattt
    18901 tgaaattata aattggcata attttgatag aagtgtggca aaggcagtta aaagtgtatg
    18961 tgataaagtg ggtataaaga aacttgggtt tgaaagaact aatatagtgt ttgataaata
    19021 tgaagaactt aaaaatctga ttgagaagga taatggagaa ttaataccta cagaaaatat
    19081 tgtagagact ttgagatatg taaaagacaa agatgaaatc aaaaatacaa gaaaagcttg
    19141 tgaaatagcg gataaggcat tagaagaatt gattccacat ataaaagctg gagttagcga
    19201 aattgaactg gctacaaagt tagagtactt tatgaaaatg aacggagcac aaaatatagg
    19261 gtttgaaact atactgatat caggagcaaa gacttcattg cttcatggta agcctagtga
    19321 taagattata gaaaaaggtg attttgtact tatagactat ggcgctatgt ataatggcta
    19381 tatatctgat acaactagaa cttttatagt tggaggagct tctgaaaagc aattagaaat
    19441 atataattta gttaaggaag ctcaaaatgt aggggttgag aacatgaaag ctggtgtaca
    19501 tgcgacaatt ccagatgctg agataagaaa agttgtcaaa aaatatgaag actattacta
    19561 tcaaggtata ggacatggag tcggtagaga tgttcatgaa gaacctttca ttggtaatta
    19621 tggtgataaa atcatagaag agggatgtat aataacaatg gaaccaggta tttatttccc
    19681 tggatggggt ggagttagaa tagaagatac tgttttgatt actaaaaatg gtccagaaag
    19741 acttactaaa ttccctaaag atttgatgat tttagacaaa taatttaatt tatatatatt
    19801 tttttacata aaatataaca taatctcact attaagctct atttacatat tatagtaaag
    19861 agggcttaat tttattgtga gaggtgatag tatatatgta taaaaaacat aaatttataa
    19921 aaaatataaa aaaagcctgg tataaaatca aattaattga tagatgcctt ataattgtaa
    19981 tggcaatatt aatgtttcaa tctatatata atttatttgt taatgaagta aattcacaag
    20041 atactacaac tattgatgta gtagtaagaa ccacttcagc agctatattt gggtattttt
    20101 taagtgctaa ttttataaag agacctttaa ggaagtatac aaatactgat gtatcttcaa
    20161 atctttttat agataataat ttatccccaa aaggtcaaga gtctaatcaa aataatataa
    20221 tgaatgtcaa aaataccatt ggatttactt cagaagctga taattatgaa aagaaaatac
    20281 ctattaataa taatgaaggt tttgaagaag gagaaactag tgaattgcaa attattatag
    20341 ctactgtaat ttgtatagta gcattaacag tactttttgt tgtaagaaat tttacaacta
    20401 caacatcagc ttctttaggt actatttcac agatgagaga ttttgtctca ggatgcgtgg
    20461 gttttttgct aggttgtcca agtaaacctg attaaaaaag tattatcaat aaaaggtatt
    20521 ttaaaaagta tcagtataat aatatttaga tttaaatgta tacaattata attctaaaca
    20581 ttctatattg tatttttata ctgatactct aaactatact atttattaaa atattttaac
    20641 attgtattta aacaaaatat tatatctgta taatataaat ttagtttata tgaatagatg
    20701 ccatatactc aataaaaagt ttcatactat gagatatgta ttgatttctt ctccaaagaa
    20761 ccccaatatt cgcagtaatt gcacgttcta atggtattga acagagattt tctgtatttt
    20821 gaaatatttc tttatacaat attgttgaag ccaagtcatt ttctagtaaa taacgtatgg
    20881 tagaaagttg atttgagtgt aaaattatat ttggcttagc tttttcaagt ctttttgtaa
    20941 taatcttata gtgataagaa ccttcagata agattacaaa aggtacattt tttaacatat
    21001 aattcgatat aatgggatgt tttgccaatt catttttggg atttgtaact aagtataatt
    21061 ctgttttata gattgtcttt gaatctatat catcataaat atcactgtca attataccaa
    21121 tacaaaaatc aagtgtagat tcttcaatca ttttagctcc aacaaatgtt ggaacttcaa
    21181 aaattttaag ctctatatct ggataaatta cattaaatct aggaagtatt tttgaaagca
    21241 aaaacgtacc taaaactgta ggaataccta ttttgagaga agcttttctc ttttttccta
    21301 aatcaagtgc gtcacattga aaatcctcaa attgttttat aaaatgtttt gacttgtcta
    21361 aaaaaatttt tccttcaggc gttaactcta ttttattacc tattctgtaa aaaagttcaa
    21421 agccaagttc tttttctaat tctttaattg ctaatgttat ggatggttga gaaatatgaa
    21481 tactttccgc agccttagtc atgtttttat atttacagac agtttgaaaa tagtataaat
    21541 agtttatatt catttggtat tagttccttt catttacata ttagtgacaa gtatataatt
    21601 aacaatagca taagttttgt atattgttgt aaaaaaatag aatagtttta aactatcata
    21661 gcataagttt ttgatatttt caatctatta agaatagtgc tattattaaa ttaacaaaag
    21721 agaatagctg gtatggttta gagtaaatca atctatatta cagttatatc aacgaatatc
    21781 aaaaaattat ctattagaaa ataaaaatta tttttacaat taaattgtta attaaatcat
    21841 aaagaaaaaa gtgatattaa caagataaaa tttttttaat cagaaatgtt aatttaataa
    21901 caaaatagag tattggggag aaaggagaaa tatatttttt aataatattt gttaatttaa
    21961 taacgaaaaa tgaaagggag tgatttagtg aagtttgaat tgatggattt tttaatgaat
    22021 ccttttgtcc ttatgtttgc tgcagtaatt acaggaattt tatttggaaa aattaaattt
    22081 gggaagttta attttggggt atcaggagca ttatttacag gattatttat tggatggctt
    22141 gcatatagct tggggaatct aataatagca aaaggggaaa cagcagcagg atataaggca
    22201 gcaactgtaa tgatgggtaa tggaattatt tcttctgact tctttgattt ctttttaata
    22261 atatttgttg cagcagttgg tctattggca gcaaaagata tgaaagctgt attaaagaag
    22321 tatggagcaa gatttgtaat tttaggagtt cttataactt ttataggtgg ctctatgact
    22381 tatgcaatga ctttactatc aagtgataaa ggaagtagtg cttatgaagt ttctggcgtt
    22441 tacacaggag cattaacaag ttcgcctgga ttagctgctg ctttagaaac agctggtaaa
    22501 catgcagaag atgtatcaaa agaatttgaa aaagcttcaa taaaagataa aaaagaaatt
    22561 ttaaaagtag tcgacccaga aggcaaattg gatgtgaata caactacatc actgactcaa
    22621 gagcaaatag ataagtatat tgcatatgct gaagctggtg ttggtatagg tcatgcagtc
    22681 gcatatcctt ttggagtttt aatagtaatc ttaggtgtaa atttccttcc aaaattattt
    22741 aggatggacc taaaagaaga aagaagaaaa tatgagaagg aaatgaagga agcaagagat
    22801 agtgtttctg gtaaaacgat accagaagtt ccatttaata taataacatt tttcttgaca
    22861 tgtttagctg gatacttagt tggaggaata catgtattta tgggaccact agggtacttc
    22921 acactaggcg caacaggagg ttcactaatt gtatctctag ttcttggata tataggtaag
    22981 ataggggtag taaatttcag aatggaagaa aaggttttaa atattttaaa acaaataggg
    23041 ctagtatttt tcttagcaat agttggatta agatatggtg gaaaagtagt tgattcaatt
    23101 atgacatctg gtatgcacct agccttagta gcaatagcag taggtgttac agctatgatg
    23161 ataggattct tagttggcaa atacgtattt aaattaaatt ggattttatt atctggagct
    23221 gtctgtggtg gtatgacatc aacaccaggt cttggagctg ctgttgatgc tcttgatagt
    23281 gatgacccag cagcaggtta tggtgctacg tatccatttg cattattaac taaagttata
    23341 ttggttatag ttttacacaa acttccaatg taaataagat tttatatggg gggatattta
    23401 tgaataatga aggagcattt gaagaattaa gtggaagatt aagaaataaa gaattgtgtg
    23461 atttaataat gaatgcagat gaagctgcta aaattataga ggatggcatg gttattggag
    23521 ttagtggatt tacaccttca ggctatccaa aagcagtacc attagcagtt tcagaaagag
    23581 ctaaaagtgg agaaaagata aaattaaccg tatattcagg agcatcttta ggtccagaag
    23641 ttgatggagc atggtcagaa gcaggtataa ttgaaagaag acttccttat caaactaatt
    23701 caatacttag aaataatatt aataaaggtg ttgttgatta tatagatatg cacctaagtc
    23761 attctactca atttttaaat tatggaacta tacctaaagt agatgtagct atagtagaag
    23821 cacttgcaat aactgaagaa ggaaatatta taccaactag tgggataggt aattctccat
    23881 cttttataaa aagtgcagat aaagtaatag tagagataaa tcttgcaaaa ccaatggaaa
    23941 tggaaggtat ggcagatata tatattacgg aaaatccacc aaatagaaaa ccaatagaaa
    24001 taaatcatcc taaggataga attggaacaa catatatacc ttgtggtttg gataaaattg
    24061 caggagtagt aattaccaat atgcaagata agacccgtcc attgggagtt gtagatgaag
    24121 cttcgaaaaa aatatcaaat aatattattg cttttttaag agaagaagta aaggcaggca
    24181 gattatcaaa gaatttactt cctcttcaat ctggtgtagg aagtgtagca aatgcagtat
    24241 tatatggatt atgtgaatct gaatttgaaa atttaacttg ttatacagag gttgtacagg
    24301 actctatgtt ggatttaata agaatgggta aagttacaat ggcatctact acatctgtaa
    24361 gtccatctcc agaaggatta ataaaatttg aaaaagatat tgattttttt aaggataaga
    24421 ttatattaag acctcaagaa attagtaaca atccagaaat agccagaaga attggtgtaa
    24481 ttgcaatgaa cactgcaatt gaagttgata tttatggaaa tgtaaattct actcacataa
    24541 tggggtcaaa gatgatgaat ggaattggtg gttctggtga ttttgctaga aatggagcaa
    24601 taactatatt tagtactgag tcaattgcaa aaaatggtga tatatcatca atagtaccta
    24661 tggtatctca cgtagaccac actgaacatg atgtaatggt aattgtaaca gaacaaggat
    24721 atgctgattt aagaggtctt gcacctagag aaagagctat aaaaataata gaaaattgtg
    24781 cccatccaga ttataaagaa caacttaggg attatttaaa tagagcttgt aaaagtggag
    24841 caaaacaaac acctcacata ttggacgaag ctttatcatg gcatagtaaa tttatgagta
    24901 caggtacaat gaaaaaggct gaaactttta aaagtgcatt ataaaatatt taataaattg
    24961 gaggtagatt ttaaatggaa aaagcagtgg aaaactttga agatttaagt aaggaataca
    25021 ttaatggtta tattgagaga gcaagaaaag ctcaaagaga gtttgaatgt tatactcaag
    25081 aacaagtaga taaaatagta aaaatagttg gaaaagtagt gtattataat gctgaatatt
    25141 tagctaaact tgctgtagaa gagactggaa tgggagtata tgaagataag gtagctaaaa
    25201 ataaaagcaa agcaaaagtt atatataaca atttaaaaga taaaaaatca gttggtataa
    25261 ttgatataga tagagaaact ggaataacaa aggtagctaa accagttgga gtagtagcgg
    25321 caataactcc ttgtacaaat ccaatcgtta cacctatgag caatgcaatg tttgcactta
    25381 agggaagaaa tgcaattatt atcacacctc atcataaagc aattggatgt agtacaaaaa
    25441 ctgtagagat gattaatgaa gaattagaaa aaataggagc tccagaaaat ttaatacaaa
    25501 tacttgacca acaatcaaga gaaaatacaa gaaatttaat ttcatcagca gatgtagtaa
    25561 ttgccactgg aggaatggga atggtaaaag ctgcgtactc aagtggaaaa ccagcacttg
    25621 gtgtaggggc aggaaatgtt caatgtatca tagatagaga tgtagacatt aaagaagcag
    25681 tacctaaaat catagcagga agaatttttg ataatggtat catttgttca ggtgaacagt
    25741 cagtaatagt agcagaagaa atgtttgata aaataatgga tgaatttaaa aataataaag
    25801 gttttatagt tagagataaa gtacaaaaag aagctttcag aaatgctatg tttgtaaata
    25861 aatctatgaa taaagatgca gtaggtcaat cagttcatac aattgcaaaa attgcaggtg
    25921 ttgagatacc agaagataca aaaataatag taatagaagc tgatggacca ggagaagagg
    25981 atattattgc aaaagaaaaa atgtgtccag ttatatctgc atacaagtat aaaagttttg
    26041 aagaaggtgt agctattgca aaagcaaatt taaatgtaga aggaaaagga catagtgtat
    26101 ctatacattc aaatacagtt aaaaatatag aatatgcagg agaaaatata gaagtatcaa
    26161 gatttgttat taatcaatgt tgtgctacta gtgctggagg aagtttcttt aatggtcttg
    26221 cacctactaa tactctaggt tgtggttctt ggggaaataa cagcatctct gaaaatcttg
    26281 attataagca tctaattaat atttctagaa ttgcttatta tatgccagaa aatgaagtcc
    26341 ctacagatga agaactttgg ggataaaatt tagacaaaaa atattcattc taaattaaag
    26401 taggaatata ttttaattta gaatgaattt aagacataaa ttaaatttaa attagtttat
    26461 tggactaaat ttaaaatatt aagagacata tagaagaagg aagtattata atttaaaata
    26521 aataaaaaaa tctataaaag agaaggagtg gttttattat ggcattaatg acaggagctc
    26581 aatatattga aagtttgaga aaattaaata ctaaggttta tatgtttggt gaagaggtta
    26641 agaattgggt tgaccatcct atgattagac catctataaa ttgtgtagca gcaacttatg
    26701 acttagctca tgacccagaa tatgcagatt taatgacagt tacttcaaat atcacaggtg
    26761 aaaaaataaa tagatttggt catcttcatc aaagtgttga tgatttgata aaaaaagtaa
    26821 aaatgcaaag actatgtggt caaaaaacag cttcttgttt ccaaagatgt gttggtatgg
    26881 atgcgtttaa tgcagtgtat tcaacaacat ttgagtgtga taaagcccat ggaactaatt
    26941 atcatgataa ttttgtaaaa tacttaactt atatacaaga aaacgattta gtagttgatg
    27001 gagctatgac tgaccctaag ggagatagaa gtctatcacc tagtgctcaa cctgacccag
    27061 atatgttcct tcatatagta gaaagaagag aagatgggat aattgtaaga ggagcaaagg
    27121 cacatcaaac tggttcaata aattcacatg agcaccttat aatgcctact ataagtatga
    27181 ctgaagcaga taaagactac gctgtatcat ttgcagttcc ttcagatgct gaaggagtgt
    27241 ttatgattta tggaagacaa tcttgtgaca ctagaaaatt agaagaaggt gcagatgttg
    27301 atttaggaaa taaggagttt ggaggtcaag aagctttagt agtatttgat aatgtatttg
    27361 ttccaaatga tagaattttc ttatgtggag aatgggattt ctctggtatg ttagtagaga
    27421 gatttgcagg ataccataga caaagttatg gtggatgtaa agtaggagta ggtgatgtaa
    27481 taataggagc agcagcttta gcagctgatt ataatggagc aaacaaagct tcacatataa
    27541 aagataaact aatagaaatg actcacctta atgaaagctt atattgctgt ggtatagcat
    27601 gttcatcaga aggtcacaaa acagaagctg gaaactatca aatagattta cttcttgcaa
    27661 atgtgtgtaa acaaaatgta actagatttc cttatgaaat agtaagactt gcagaagata
    27721 tagctggagg gcttatggtt actatgcctt cagaaaaaga ctttaaatca gaccttaaag
    27781 ttggaacaag tggaatgact attggcgaag tatgtaacaa gtattttaaa gcttcttcag
    27841 tggcttcaac agaagaaaga atgagaatat taagattctt agaaaatata tgccttggtt
    27901 catctgctgt tggttataga actgagtcaa tgcatggggc aggttcacca caagctcaaa
    27961 gaataatgat ttcaagacaa ggaaacataa atcaaaagaa agaattagct aagaaaattg
    28021 cgggaataaa aaaagaggaa gctttaaatt tataaaaagt tgatatataa agggaaatta
    28081 tatattaatg tgtgttaatt aatgaaaaaa tatgtagtat aagagatagt tataaaatta
    28141 tggtggtttt tagctaagta aagaaatttt taataaggag aatttacaca tgacctgtgg
    28201 agttagattt tgtgggggct gtaaccctag atacgaaaga gggaaggctc ttacaataat
    28261 caaagagcac ttcaatggta gggtagattt tagtatagca caagagaacg tcgagtatga
    28321 tttgttactt ataattggag gatgcgcaac tctatgcgca tcctatcaac aatataaagt
    28381 gaaaaatgaa tgtgttaaga tgtgggattt tagccatata gaaagagtta taaatgtgat
    28441 tgaatcagca atttgtgaaa cttaaaaaag ctaaatgggg ggatttcatt gagttggcaa
    28501 gaattgtatc aaagtaaatt atgtagtgct acagaagctg taaaacagat taaaaacggt
    28561 gatactgtag tatttgcaca ttgtgtaggt gaacctcctg cactagtaga agctatgatt
    28621 gaaaatgctg aacaatataa agatgttgag attaaacata tggtaagctt gggtagtggt
    28681 ggatatactg ctaagggaat ggaagctcat tttagagtaa atccaatgtt tgtaagtgga
    28741 aatgtaagaa aggcaattga aaatggagat ggtgatttta cacctgcatt cttccatgaa
    28801 gtaccaaagt tgcttagaga aaaaagatta aaatgtgatg ttgttttagc acaggtaact
    28861 ccaccagatg aacatgggta ttgtagctta ggaacatcag ttgattatac ttatgaagcc
    28921 attaaaactg ctagaacagt aattgttcaa gtgaatgacc agtttcctag aacctatggt
    28981 gaggtagtcc atgtatcaga gtttgactat atagtggaaa aatcacaacc tttatttgaa
    29041 ctacaacctg caaagattgg agaagtagaa gaagcaattg gtaaaaattg tgcttcatta
    29101 attgaagatg gctctacttt acagctaggg attggtggga taccagatgc agttatgtta
    29161 tttttaactg ataaaaagga tttagggatt cattcagaaa tgatttcaga tgggactttg
    29221 gccctttatg aaaaaggtgt tataaatggt aaatataaaa attttgataa agaaaaaatg
    29281 acagttacct tcttaatggg tactaaaaaa ttatatgact ttgcaaataa taaccctgca
    29341 gtagaggtaa aaccagtaga ctatgtgaat catccagcaa ttataatgaa acaacataag
    29401 atggtttcta taaattctgc tatacaagtt gatttaatgg gacaggtagt tgcagaggct
    29461 atgggactta gacaatttag tggtgttgga ggtcaagttg actttatacg tggagtgtct
    29521 atgggtgaag atggaaaggc aattatagct atgccttcaa taactacaaa aaaagatggt
    29581 acagtaattt caaaaatcgt atctattgtg gatgaaggtg cacctataac gacatcaaga
    29641 aatgatgttg actatattgt aacagaatac ggtatagcag aattaaaagg aaaatctctt
    29701 agagaaagag caagaaattt aataaatata gctcatccaa gtgtaagaga acatctagca
    29761 gtagaatttg aaaagagatt taaggagaaa tattaataat agaggggtgt taataatgga
    29821 agcacttaga gtagttccaa agatatttta ctttgataca tttaaagaat ttaatgaaga
    29881 atttaaaata ggaaaaaatg atttagtaat tactaatgag tttatatatg agccatatat
    29941 gaaacctctt ggaatagata caaatttaat ttttcaagaa aagtttggaa caggtgaacc
    30001 atctgatgaa atgatagact ccatgacaaa ggaaatgaag aaatacaatt ttgatagaat
    30061 tattgcattt ggaggaggaa caatagttga tatatgtaag atattagcac ttgatgttcc
    30121 agaaaagtct attgatttat ttgaaggaga agtatcacct aaaaaagtaa aagagttagt
    30181 agtagttcct acaacatgtg gtacaggtag tgaagtaaca aatgtagcta ttgcagagtt
    30241 aaaatctaaa catactaaaa aggggcttgc agtagaagaa acatatgcag actatgcagt
    30301 gttaattcca gagacagtaa aagggttacc ttacaaattc tttgttacaa gttcggtaga
    30361 tgctcttata catgctattg agtcttatct ttctccaaaa gcaagtccat ttacagagat
    30421 gtattctcta caagcaataa aaatgattat ggatgggtat aagaagatag ttgataaagg
    30481 agaagaagag agatttaatc atcttagaga ctttgtactt gcatcaaatt atgcaggaat
    30541 tgcttttgga aatgctggtt gtgcagcagt tcatgcacta tcttattcaa taggtggagc
    30601 tttccatgtt gcacatggtg aagcaaatta tcagttcttt actgaagtat ttaagatgta
    30661 ttctagaaaa aaacctaatg gaaaaattat aaaatgtaca aaaattttag cagatgccct
    30721 tgaatgtgac cctaactgtg atgtttatgg agaattagaa aagtttttaa ataaattaat
    30781 agctaaaaaa gctttgagag aatatggaat ggtggaaagt cagatagatg aatttacaga
    30841 tagtacaata gcaaatcaac aacgactact tgctaataat tatgtagagc ttagtagaga
    30901 ggaaataaga gagatatttg caaatcttta ttaatacaat tttttattta tatgtatagt
    30961 caacataaac aatgctaatt atatttatgt acaaataaaa ataagggaac ttaaatttta
    31021 attaaagtta agttctctta tttttatatg gagttaaata taagtatatt tttaatttaa
    31081 aatatgctta tatttaagtc tattacttta tttgaaacaa tttgtagaat ttcaaattcc
    31141 ttgacagtaa taagtaagta attgtataat caaactgcaa tatattaata atattagtga
    31201 aaatgaaagg attataaaat atgtataaat cagaaaattt ttcaaaaaaa tctgtattaa
    31261 taaattcatt tttaagtaaa tttttaaaaa taattggatg gggtttggta agcttattta
    31321 cattatcagc cttagtactt tcaactattt ccattataat tggatgggct gtaatacttc
    31381 ctattcaagt atgtgcagta tttttactta taggaggagt ttgtattttt aaaggtaaga
    31441 gattaggaga ccaaatttct cgttataaaa aatattgtac aattataaac aatagaaata
    31501 ttattccagt agaacttatt gcagaaaaca catctaaatc attaaatttt ataataaaag
    31561 atgtacaaaa gatgatagac aaaaactact ttataaatac ttatattgat aaaagaaata
    31621 atcagattgt gttaacaaat gaagattttg ttccaccaag atatgaagat gtagcatatg
    31681 aagtcaaaga agaagaaaag actgatgaat ccaatgaagt tgatacaatt ataaataaag
    31741 gaatgaatta tctaacacaa ataaaagaag ctaataaaaa tattaaatca gaatcaatgg
    31801 gcgataaaat tgtacaagta gaagatgtta caagtaaaat atttgatgtt gtaaaacatg
    31861 acccatcaaa acttactcag atacaaaaat ttatggatta ttatcttcca accactttaa
    31921 aactattaaa ttcatatcat acattggaag aacaaggaat tgatagagaa aatataacaa
    31981 caactatgga aagtattgaa aatacaatga atacgattgt agtagctttt gaaaatcaat
    32041 tggactatct gtttgaagat gaagccattg atatttctac agatattact gttttagaga
    32101 atatgttagt ccaagaagga ttaacgagtg gtaaatttta acttcaatag aaaaaatatt
    32161 ctgttatttt taaacaatta attagtcata aagtataaaa tgtgtattat ataagttaat
    32221 cagtacttta tgaaggaaga ggagagaaag atacaatgaa taatttagat gatataccag
    32281 ttatgccaac tttgacactt gacccatttg gagaagaatc aaacactaat gatatagata
    32341 attctgactt attaatgaaa aaggatgaaa aagacccaga agaagaaaaa ctgtctgaat
    32401 ctgaacgcaa gatggtaaag gaatttgcgg aaaaaataga tattacaaat acaaatatga
    32461 ttttgcaata tggagctgga gctcaaaaaa aggtagcaag tttttctgaa acagctttaa
    32521 agagtgtaaa aacaagagat ttaggagaag ttggagatat gcttacaaat ctagttactg
    32581 atttaaaagc attttcagca gatgaaaaag aacaaagtgg ttttttagga atttttaaaa
    32641 aggcaaataa taaaatttca aatttaaaaa ctaagtatga tagtgcagag gtaaatgtgg
    32701 ataaagtaag caaagagctt caaaagcatc aggttaaaat attgaaagat atagcaatgc
    32761 ttgataagat gtatgaattg aacttagctt actcaaaaga gcttactatg tatattatag
    32821 caggaaaaca aaaattaaag gatatgaaag aatatgaaat gccaaagttg agagaaaaag
    32881 ctcgtttatc tggttcaaca gaagatgctc aatctgtaaa tgatatggtt tcactttgtg
    32941 acagatttga aaagaagatt catgacttag aattaactag aatggtatcg cttcaaatgg
    33001 ctcctcagat aagattggtt caaaatagta ataatctgat ggcagaaaag attcaatcaa
    33061 ctatagtaaa taccattcca ttatggaaaa atcaaatagt gcttgctctt ggaatttctc
    33121 acttaaatca agctatgaaa gcacagcgtg aagttagtaa tatgacaaat gagcttttat
    33181 taaagaatgc agaaactttg aagatgggaa caatagaaac agcaaaagaa tcagaaagag
    33241 gaattgtaga tatagacaca attaagaaaa ctaatcaatc tctaatatct actattgatg
    33301 aggtagttaa aatacaacat gatggtagaa taaagagaca agaagcagaa gttgaattat
    33361 caaaaataga aaatgaactt aaaagtaagc ttcttgaatt tacagttaaa taaaaattat
    33421 actaatataa aaagagactg tctcaaaata gagataagtc tctttttcat tgtttaaatg
    33481 attaaagtat atttatatta taaatttcta ttatctttta acataacatc atgagctcca
    33541 ccttcaacta tactagtaga agatacaagt gtaagtttag catttttttg aagttcagga
    33601 atgctaagtg ctccacagtt acacatagta gaacgaactt tacttaaaga aagagttaca
    33661 ttgtctttca aagaaccagc ataaggcaca taagagtcaa caccttcttc aaaagaaagt
    33721 ttcttatctc caccaaggtc atatctttgc cagtttctag cacgagcaga accttctccc
    33781 caatactctt tcatataact accatttata ttaactttgt tagttggact ctcatcaaaa
    33841 cgagagaagt atctaccaag cattataaaa tctgcaccca tagctaaagc taaagttata
    33901 tgatggtcat aaacgatacc accatcagag caaattggaa tataaatacc tgttttttca
    33961 aagtattcgt ctctagcttg agcaacttca attatagaag tagcttgtcc acgtcctata
    34021 cctttttgtt cacgagttat acatatagaa ccaccaccaa taccaacttt aacgaagtca
    34081 gcaccagctt cagctaggta aaggaatcct tcgcggtcaa ctacatttcc agctcctacc
    34141 ttaactgtat caccatattt ttcacgaatg aaatcaagtg ttattttttg ccactcagaa
    34201 aaaccttctg aagaatctat acataaaaca tcagcaccag cttcaactaa agcaggaaca
    34261 cgttcagcaa agtctctagt attaatacca gctccaacta catatctctt agaagaatct
    34321 aaaagttcaa gtggattttc tttatgagaa gagtaatctt tacggaatac catatacata
    34381 agattaccat tatcatcaag tacaggaaga gaattaagtt tgtggtccca tataatattg
    34441 tttgcttcct ttaaagtaac atctttgtta gcataaacta tagatgacat aggagtcata
    34501 aattcactaa ctttagtatc taaatccata cgacttatac gataatcacg acttgcaaca
    34561 atacccaata atttaccagt tgaagttcca tcttcagtaa cagctactgt agaatgtcca
    34621 gttttttctt ttaagtcaag tatgtctttt aatgtgttat caggtttaat atttgaatca
    34681 cttacaacaa aaccagcttt gtgggattta acacgtgcta ccatagcagc ttcattttca
    34741 atagtttgag aaccgtatat aaatgaaata ccaccttctt tagcaagtgc aatagccatt
    34801 ttgtcatcag aaacagattg cataatagca gaagtcaaag gtatattcat ataaatatct
    34861 gcttcctcgc cctttttaaa tttagtaaca ggtgttttta aacttacatt agcaggtata
    34921 caatcagtag aagagaaacc tggtacaaga aggtattcac tgaaagtatg tgatggtgta
    34981 tcaaaataaa acgccatttt ttaatcactc cttatatttt tatacttttt ataattgata
    35041 gtattaatta taacttattc tttaatttta ttaaagtatt ttgtcaaata ttgagatttt
    35101 tgaaaaattt aaactatttt aattaaattt gtttaatata tattgcctta tagggtaaag
    35161 ttgtatatta aataatgttt agtgtttaat agtagctaat ttaaaattaa tctttagtga
    35221 ttcatataat tacatttgta tagtttacat tgataatatt actagagagt gcataaacct
    35281 caaaaataca gataaattaa atctatatta gtatatccaa aagtacattt aataaattta
    35341 tttaactgaa ttattatttt catttctatt aaatttaaat atttt
//
LOCUS       DAMJVK010000036        34873 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.036, whole genome shotgun sequence.
ACCESSION   DAMJVK010000036 DAMJVK010000000
VERSION     DAMJVK010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34873
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.036"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(441..2828)
                     /gene="cadA"
                     /locus_tag="PC414_001825"
     CDS             complement(441..2828)
                     /gene="cadA"
                     /locus_tag="PC414_001825"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HDF2464222.1"
                     /translation="MEANNSIKKEFILGGLNCAHCAEEINNKVSKLQEVKSSNLNFIN
                     KKLTVNIKESFNEDTTIEKIIDIIDSTEPGLDIQISSKENAASKTSIKKELILGGLNC
                     AHCAEEINNKVSKLKEVESSNLNFVNKKLTVNISNNFEEDDVINKIKEIINSTEPGLD
                     IQVGSTDKVKGRTTEKSGAVNDTNKKELIPLIIGALVYIFGIYQTATGYESQFSNIVF
                     IVAYVIVGGDVLLRAIRNISKGRVFDENFLMALATVGALAIGELSEAVGVMLFYKVGE
                     YLQGVAVGKSRKSITSLMQIRPDYANLKVNSEVKVVSPEEVNVGDIIVVKPGEKVPLD
                     GVVVDGVSMLDTSALTGESVLREVEKGDEILSGVINKNALLSIEVTKSFGESTVSKIL
                     DLVENSSIKKSKTENFISKFSRYYTPIVVIAALLIAFVPPLVISGEVFSDWLYRGLIF
                     LVVSCPCALVLSIPLSFFSGIGFASKNGILIKGSNYLEALRSVDTVVFDKTGTLTKGV
                     FNVTKLNPEGISEEELLEYAAIAEVNSNHPIAKSILSYYNKKIDLDTIDSYEEIAAYG
                     IRVKHNGNFILAGNEKLMKKENISYSSAKEVGTVVYIAVDKAYRGYIVISDEVKEDSK
                     NAIRSLKEIGVKEVVMLTGDNEKVAKNIAQELELDTVYSNLLPNEKVDRLEDLYEGRT
                     EKEKIAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVLMTDEPSKISKAIEIA
                     NKTNKIVWQNIIFALGVKIIVMILGAGGVATMWEAIFADVGVALIAVVNAMRAMR"
     gene            complement(2851..3216)
                     /locus_tag="PC414_001826"
     CDS             complement(2851..3216)
                     /locus_tag="PC414_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2464223.1"
                     /translation="MREEINDCNCNIVHEEIVTEAKSTMPDEEMLYDLAELFKVFGDT
                     TRVKILYALFANEMCVCDIASLLNMTHSAISHQLRVLKQARLVKFRREGKTVYYSLDD
                     SHISQIFDCGLNHIRETYK"
     gene            3415..4263
                     /locus_tag="PC414_001827"
     CDS             3415..4263
                     /locus_tag="PC414_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464224.1"
                     /translation="MSNKKKKDLDTSYMPYLDPANYADYITNEEIPQDELRNDPQISN
                     GYTKIPNNQNINNLYPNNVNHYPKSSNSPNNLPNTNNTSGNMNYNTSATNNMPNNMNN
                     NTGMPNNMSNNMNSNMGMPNNMPNNMNSNMGMPNNMPNNMNSNMGIPNNVPNNMNSNM
                     GMPNNMSNNMNSNMGMPNNMSGNMNNNMYGNTGMPNNMNSNMGYNMNNTMGIPNFSCN
                     QNMPPNVLMMIPGVICHNTMQGMPVVMPSTMPPNIYPTPYGSSNMSIQGIPQATNIEE
                     FDEEEM"
     gene            complement(4700..5788)
                     /locus_tag="PC414_001828"
     CDS             complement(4700..5788)
                     /locus_tag="PC414_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464225.1"
                     /translation="MNFNFAKATNSRLMGSLGLIINWIDDENNHFCQYFLLDAEGLGL
                     ADYVSLNNPTQEEAYMEEERLMGGFGSDRVELTKDESLFLVSYFGNKNLYYDKLLPGD
                     KCEYIDIIKNYKTDLTIEKLYNKICKRVDEEVEFINYMTMRFIAWDRESLKYFSGSDE
                     IANMHITNINGTLLKNVVSDKGQGRYISEALYEDNDGYYICKIAFCISKCYETGFRIN
                     SLLVTDKEPMYDFEVFDEISKSEFVSVYSVNSPEEFAGVFYRENPFLLKSNMNEGIFF
                     TRFNFNNDHVKENVYIINNDMKAIYYLMGNKFFIGTYNENDCNYINEISQSNYSDYIK
                     FEEKFFFEQNALYDFAESGSLDFDDFLE"
     gene            5913..6641
                     /locus_tag="PC414_001829"
     CDS             5913..6641
                     /locus_tag="PC414_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HDF2464226.1"
                     /translation="MKNIAAFFDIDGTLYRDSLMVEHFKKLIKYDIIDQKNWYKHARD
                     TFMDWDKRQGNYDDYLLEICDLYVDSLKGLDKTCINFTSDQVIKLKADRVYKYTRSRI
                     EWHLNQGHIVIFISGSPGFLVEKMAKKYNVTDFLGSDYVFENNIFTGTVIPMWDSISK
                     NNAINDFVVKYDLDLSNSYAYGDTNGDINMLKRVGNPIAINPTKELLSQISLDEEISK
                     NVEIIVERKDIIYSLSTDVNIVDI"
     gene            complement(6845..7336)
                     /locus_tag="PC414_001830"
     CDS             complement(6845..7336)
                     /locus_tag="PC414_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464227.1"
                     /translation="MSIKVAYGGVLLALNVILLTLTNIIPVNTLFIMGLASLLVSIVI
                     MEWGFKSGIAFYIGSIVLGFIVMASKSQWIVYSLTFGIYGIVKYLIEKDRSIYIEYFM
                     KLVFANIMILILYFLLRTIVYIPINIFIIGSFEIAFIVYDYVYSSFIGYYNNRLRKML
                     FKK"
     gene            complement(7347..7790)
                     /locus_tag="PC414_001831"
     CDS             complement(7347..7790)
                     /locus_tag="PC414_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464228.1"
                     /translation="MYSNTYNNENFMRARAFIENSEFKKAYDFLKTLTDKCAEWYYLT
                     GFSAMNIGYYEEGEDFLKRAKFMEPENSEYSDALRSYTQYRNDYSNRADNYNRRRRND
                     LDGCCCCCCDDCCCCLGDDCCENCAKLWCLDSCCECFGGDLITCC"
     gene            complement(7780..8619)
                     /locus_tag="PC414_001832"
     CDS             complement(7780..8619)
                     /locus_tag="PC414_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464229.1"
                     /translation="MFGYVKINKMDLTFREYDYYKAYYCGLCKYLKRNHGEISRFSLN
                     YDITFLIVLLTAVYNPESISTEEVCIVNPFKKKKVITNDITEYAASMNILLTYYKLED
                     NLMDDKRIKDKLAYYIYKNKLKLAYEKYPEKAEYIKQQLNELNKLEKDKNINIDEVSS
                     IFGNIMGEVFVYKKDENERNLRMIGFNIGKYIYLLDAYEDLDEDFKKGRYNPFIEYID
                     KNDELKEKVKKIIVTSLGFLARGIDNLNLNKNVGIIENIIYSGVYLRYINILESRGGK
                     NVQ"
     gene            complement(8838..10223)
                     /locus_tag="PC414_001833"
     CDS             complement(8838..10223)
                     /locus_tag="PC414_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2464230.1"
                     /translation="MENLNKKFLMEYIDEIQGELRDISDKIWKNPELQYKEYYASNLQ
                     KEYLKKYGFSIDELEGMDTAFAASYGEGRPVIAVLGEYDALDSLSQKVSTIKEPIVDG
                     GAGHGCGHNLLGTGSIGAVIAVKELIKLKKLKGTIKYYGCPAEEDLSGKVLMIKKGFF
                     DGIDCAFSWHPFDINTPIRIPTLANYSVKFRYNGISAHAAQAPYNGRSALDAVELMNI
                     GCNYLREHIFDNCRVHYVTTNGGKMPNIVPDFAEVWYYIRGVKMEHVRDVFGRIVDIA
                     KGAALMTGTIMEYDIISGVYDYIPNTILTDILSQNMKLVGVQNYNEDDYYFADKLAET
                     VSIDKRSSVSSVLSGNKDITKMNLHDEVTDDTFTHNNCISLSLDIGDVSYIIPTAQCS
                     CSVWPIGISAHTWQSCASAGSDMGFKAMLLASKSISCSINDVMLDELVINKAKKELKD
                     TVGSFKYIPII"
     gene            complement(10396..11139)
                     /locus_tag="PC414_001834"
     CDS             complement(10396..11139)
                     /locus_tag="PC414_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HDF2464231.1"
                     /translation="MKKVLIVGESWVKNITHIKGFDTFVTTHYEEAVKWLKEAIESGG
                     YETVHMPAHVAADSFPYKLEELNEYDCIILSDIGSNTFLLSNSTFIDCNSNPDRLELI
                     KEYVNNGGALIMVGGYMSFTGIDAKARFGETAIKDVLPITMIDKDDRVEKPAGIIPEV
                     IDSKHPVLKGIPTEWPKFLGYNKTVARDNCPVLATIGGDPFVAVGEFGKGKSAIFSSD
                     CAPHWGPKEFTDWKYYNKLWVNMLDWLTC"
     gene            complement(11159..12421)
                     /locus_tag="PC414_001835"
     CDS             complement(11159..12421)
                     /locus_tag="PC414_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2464232.1"
                     /translation="MKKILLDTLNSKKQEYIDYLKELVSIKTEDVGHGILGGFEKEGQ
                     EYIEKLANYIGFSVDRQEMSEELIKKAKNIYKEGNLGHNYQDRYNLICKYSDDLPGKT
                     IVFNGHVDTMPPGDISKWKYNPYRATEDNGKLYGLGTADMKSGLIASILAVKLIKDSG
                     LNVPGNVKIMSVVDEEGGGNGTINAVMNGIDGDCCIICEPSEQNLIVAHMGFVFFEVE
                     VKGVSLHCGSKWEGVNAIEKAMLLLQDIKELEHNWLMIYKHPLLPSPTINLGVINGGT
                     AGSTVPDKCVFNLCVHFLPNIMSYEQVVNDVTNVIMTRANGDLWLKDNKPNINIYQSG
                     LGFEMDKDSDFVVNAHKILEETLGKKLEIKGSTAGNDARLMKNLAEIPTLILGPGSIE
                     QCHSIDEYVEIKEYLDSILMYASLILNL"
     gene            complement(12605..13603)
                     /locus_tag="PC414_001836"
     CDS             complement(12605..13603)
                     /locus_tag="PC414_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HDF2464233.1"
                     /translation="MSSITKHKEEKSSPLLTKLTSVFRDQGSAAVGLVIIFIIMSVAS
                     SNFLTLDNLINVGRQISINAILAVGMTFVIITGGIDLSVGAVIALVGTFWATTVVNYN
                     APIWVGMILALAIGTILGVIKGAIISTQKLPPFIVTLAMLTIISGASFVFTGGRPISV
                     NTDAFKMLGRGYIGPIPIPVIIMIIVVIAGHFLLKRTNFGRHVHAVGGNEEAARLCGV
                     KVNKVIVKVYALAGLLTALAGIILSSRLASGSPTVGDGAELDAIAAVVLGGTNMMGGS
                     GSIVGTCIGVGIIGILGNGLNLLSVSSYNQMIIKGLVMLFAIWINNIKLKKSAKSK"
     gene            complement(13587..15098)
                     /locus_tag="PC414_001837"
     CDS             complement(13587..15098)
                     /locus_tag="PC414_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDF2464234.1"
                     /translation="MSNIILKLSNIAKEFPGVRALDNVNFELFHGEVHALLGENGAGK
                     STMIKILTGAHSKTSGKFIFEGKEIENISPDISKKIGINAIYQELTVFDELTVAQNIF
                     MGKEINGKVLTNDKKMNEEAKKIFDNMGIDINPNSLVKELSIAQKQMVEIARVLSSET
                     KVLIMDEPTSSISKKETEILFRLINDLKESGVSIIYISHRMEELFEICDRITIMRDGK
                     TISTLNTKDVSSEEELVNLMIDRKLDQFFPKRKVEIKEEIMRVENLTKNNVFNDISFN
                     IRKGEILGIGGLVGSKRSEIVEAIFGLRTYDSGKIYLNNEEVKFKTPSDAIENGLGLI
                     TEDRKGTGLFLQMSVKENTTMAGLKKISKFKSIIDRKKEKEILEKYIEALKIKTPHMN
                     QVIQSLSGGNQQKAIIARWLLLQPDILIMDEPTRGIDVNAKAEIYNLMGDLVESGVSI
                     IMISSEIPELISMSDRIMVMREGHISGFLEGEEMVENNVLKLAFGGKINEFNN"
     gene            complement(15152..16114)
                     /locus_tag="PC414_001838"
     CDS             complement(15152..16114)
                     /locus_tag="PC414_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDF2464235.1"
                     /translation="MRVRKILSLGIAGILAIGMLTGCSMEGPSKSDNKGGSDKKDLTI
                     GVSTITLQHQFFIDIDEGIKEKAKELGVKVIVNDPDQDVAKQTSAIEDFIQQNVDGMI
                     VLGTDNSAIVPAVEGAFEKMPVVTVDAVLNTENITSYVGTVSYDAGKKLGEYTKKYID
                     EKLGGKSEIAIVTDLKSQIQMQRIDGFKDALKGSANVKILNSQPGYDREESLNTVENL
                     IQSNPDVDIIYATAENSVLGAKAALESAKNKDVKIVGFDLTEEASSGITDGTILAMIQ
                     QQPKEMGRLAVEAVVKAIKGEKVEKNIPVPALLYDKENIKDFKN"
     gene            complement(16162..17052)
                     /gene="rbsK"
                     /locus_tag="PC414_001839"
     CDS             complement(16162..17052)
                     /gene="rbsK"
                     /locus_tag="PC414_001839"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDF2464236.1"
                     /translation="MGNIVVIGSVNMDMVCSVDKRPEKGETVLGNSFFTSPGGKGANQ
                     AISASKLGANVKMISCIGEDGLGEELIRNFRTNKVDYSLVSRNNHKSSGVAVITLCEN
                     DNSIVVVPGTNELVDIELIKKNEEEIKNADIVLLQLEIPLKTINYVVNFCFENRIRVL
                     LNPAPAVKLNEDIIEKVTYLTPNEHEYKIVFDTNEGIEEVLKKYPNKLVITEGKNGAR
                     FYDGEEIKHVSCISVDVQDTTGAGDTFNGALAVAITEGKNLYTAVEYAVVVSGLSVTK
                     LGAQSGMPYKEDVEKYLNNK"
     gene            complement(17094..18107)
                     /locus_tag="PC414_001840"
     CDS             complement(17094..18107)
                     /locus_tag="PC414_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDF2464237.1"
                     /translation="MSITIKEIGELAGVSKTTVSKVINNKDENISQATREKILKIMKE
                     KNYVPNKLAQSLVTKKTNTIGLLIPDIRNPFFTDVSRGVEDKANEEGYNIILCNTDED
                     AKKEYEGIRTLSERMIDGIIFAASSNTNWKEANYKDIKIPTVLIDKKISMNKETLKGI
                     VKINNFEGAYIATKHLLDIGNKKIIYLSGPLQNEIAVDRLEGYKKALIECNLSYNPAY
                     VFEGKYKIEWGQEFIKNLEKIDFDAIFCANDLIAIGVIRGLKERGLSIPNDISVVGFD
                     DIQTSSLISPSLTTVKQPSYDIGYKASEILINCLRGDKKESFDELIFKPELVIRDSTK
                     ESE"
     gene            complement(18336..19313)
                     /gene="bioB"
                     /locus_tag="PC414_001841"
     CDS             complement(18336..19313)
                     /gene="bioB"
                     /locus_tag="PC414_001841"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HDF2464238.1"
                     /translation="MEKYIIKLKNKVLREKEISYEEALNLISLDTNNKNDFDILLKSA
                     NEIREYFMGRKADLCTIMNAKSGKCSEDCKFCAQSSHYKTGVEEYSLLDYNEILNRAK
                     EMESKGVHRFSLVTSGKGMSGKEFNNILNIYEGLRENTNLKLCASLGIIDYEQAKMLK
                     SAGVTTYHHNVETCRDNFHNICTTHTYKDRIKTIKDAKKAGLDVCVGGIIGMNESEEQ
                     RLKMAFEIRELNVKSFPINILNPIKNTPMENYDVLEPMEILKTTAVFRFIIPNVYIRY
                     AGGRLSLKGYDKVGFNGGVNSAIVGDYLTTVGSGIENDKKMIIEQGFEL"
     gene            complement(19519..19782)
                     /locus_tag="PC414_001842"
     CDS             complement(19519..19782)
                     /locus_tag="PC414_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464239.1"
                     /translation="MKIYYEKENSKKANVNNIPSVFAFNFLRNFLTSKEYEETRKEYF
                     INNLTKSQVNQAMKDLKWLFREYEGLEVITMESLDGVKTRIVL"
     gene            complement(19852..20925)
                     /locus_tag="PC414_001843"
     CDS             complement(19852..20925)
                     /locus_tag="PC414_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HDF2464240.1"
                     /translation="MEKSKVYFCDLYSNSQNKNVPNNVRRLFDEAGFKDLIEKNDQVA
                     IKLHFGEKGNTTYMSPVAVRQVVDKVKDCEGKPFLTDTNTLYTGSRTNSVDHLTTAIE
                     NGFAYAVVNAPVIIADGLYSRNYENVKIDKKHFESVKIGGEIYNSSAMIVMSHFKGHE
                     AAGFGGALKNLAMGCASAAGKQMQHSDVTPVVKEKKCVGCGKCVNSCPTKAISIVDKK
                     AVIDSDVCYGCGECPTVCPTRAVTIQWESDSDVFVEKMAEYAYGAVSNKKDKVGYITF
                     VMNVTPLCDCVPWSGRPIAHDIGILASTDPVAIEQACYDLICKEMGHDVFKHEHPHVN
                     GTRIIDYACEMGMGSKEYELIKL"
     gene            complement(21173..21931)
                     /locus_tag="PC414_001844"
     CDS             complement(21173..21931)
                     /locus_tag="PC414_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2464241.1"
                     /translation="MREYIAFTKKEFKENLRNYKLFSLIILFLIFGISSPISAKFMPD
                     LIAHFAPTLKVTAAPTALDSWTQFFSNISGLGMSLTLIIFCNILSNEYSKGTLVIMLT
                     KGLSRSSIVLSKFSVTVIIMTIGFWLSFLFAYGYTIYFWPTANLNHIIFSAFNLWLIG
                     IMYISVLILGCVLFRPAFASVLLVLVATLLLSLISIPKQIAPYTPNFLMSKNIDLISG
                     KVAMPEFIIPIIITVLISVVCLLAAVILFNKKPV"
     gene            complement(21928..22845)
                     /locus_tag="PC414_001845"
     CDS             complement(21928..22845)
                     /locus_tag="PC414_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2464242.1"
                     /translation="MNIVSINGLSKGFGNRKIIDNLNFTVPEGSVFGFVGKNGAGKTT
                     TMKMVLGLLKPDSGTIDVCGEKVTYGKTSSNRHVGYLPDVPEFYNYMRPLEYLSLCGE
                     ITGLSKKEIQIRSEELLSLVGLGNEKRRIGGFSRGMKQRLGIAQALLSRPKLLICDEP
                     TSALDPVGRKEILDIMLKIKDSTTVIFSTHILSDVERICDHVAILNKGSIALSGTLSE
                     IKSMHGKDRLLLEFASNDEIQKFKSSDGIKSLLKDSEETNMEIVLHGKDIKAIQKTVI
                     STLAEMNLCPVKMELIELSLENLFLEVVK"
     gene            complement(22845..23045)
                     /locus_tag="PC414_001846"
     CDS             complement(22845..23045)
                     /locus_tag="PC414_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc_N domain-containing protein"
                     /protein_id="HDF2464243.1"
                     /translation="MENLMEYLPLLVPVIILDLILIITALVHVLRHPNYKIGNKAIWI
                     IVVLFISLIGPILYFTIGRGEE"
     gene            complement(23050..24144)
                     /locus_tag="PC414_001847"
     CDS             complement(23050..24144)
                     /locus_tag="PC414_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2464244.1"
                     /translation="MNELNIAKTLILKRKEKGITQDELANYIGVSKASVSKWETGQSY
                     PDITFLPQLATYFNITVDELICYEPQMMKEDINKLYNKLCKDFTVKPFDEVMDEIREI
                     IKKYYSCFPLIFRMGLLIVNHYDIVDEKKRELLISEALEIFIRIQETCNDIDICRQAK
                     SMEATCYILLNQPIQVIDLLQNSNFPMINESILLAQGQMMNGQMDEARETFQLGAYQN
                     LISLVQNLVGILQNADKLQMKEIERRILAISDIFELDTLSPAIMLSSYLTQAQINLIH
                     GDNEGAIKSLRKYVDLATRDIYPIIIHGDDFFNKLDRWISEIGTGITRDDTIVKAGIV
                     AAIKNNPMFSVLSENKEYKFLIEKLSLLEE"
     gene            complement(24654..24794)
                     /locus_tag="PC414_001848"
     CDS             complement(24654..24794)
                     /locus_tag="PC414_001848"
                     /inference="COORDINATES: protein motif:HMM:NF021468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HDF2464245.1"
                     /translation="MNGEGCILSNKITKECYKVGYMIREKSIDTYPDSGWGFMTWDEN
                     GE"
     gene            complement(25104..26411)
                     /locus_tag="PC414_001849"
     CDS             complement(25104..26411)
                     /locus_tag="PC414_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2464246.1"
                     /translation="MDISRSVDNSLNRTIEFLKELIKIDSQQGEPISQCPFGIGPKKS
                     LDKTLDYCASLGFSVKNIDNYIGYAEIGEGEELIGIPMHLDIVPPGEGWSVDPFSGAV
                     IDNIIYGRGVIDNKGAVSMLIHVLKNIEDMYPTINKRIRLIFGTNEETGMKCIKYYLD
                     KGEEIPSMGFTPDAMYPVVNGEKGRVHIRIEKEIKIDKSKPYIIVRGGTKENVVPSHC
                     TAKIINGIISELTTKGVAVHASNPEKGENAISKMVIKIVEDNMDFQHREDIELVSKYL
                     CSDYYGDALGINQYDEVFKNTTLNLGILKVNEEKIVCELDIRYGKNIVLNNIIDRFKK
                     VFCNGWKIEVIAHKDLHYVDESNLVLKKLLEAYEEVTDENGYTIAMGGGTYASWFKDM
                     VAFGPKFLAYKTGGHGVDERVPINHIRKNMEIYTLALIKLLEL"
     gene            complement(26476..26955)
                     /locus_tag="PC414_001850"
     CDS             complement(26476..26955)
                     /locus_tag="PC414_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HDF2464247.1"
                     /translation="MSEKMYTEKEYLEGIRGATGERAVWFYLLMKEAEKLGANPDDIC
                     KEAIYGFGKMRGQKYNVADTPGKMAEMLYNSKGQKVFEMELVENTDENGVLKFHHCPL
                     DAAWKEYGLTKEERKEICRLACYGDYGRVDCAQGVKLDFAQKCAHDDEVCELVFTKK"
     gene            complement(27002..27865)
                     /locus_tag="PC414_001851"
     CDS             complement(27002..27865)
                     /locus_tag="PC414_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDF2464248.1"
                     /translation="MQEIKNKEVKNGQGPTEVNKTRVLTKKDVTKTYLRWWWTAELSN
                     SFERMQALAVCASFTPALEKLYKRKEDLVDALKRHLQFFNTQAIWGGLIHGTVLAMEE
                     EKATEGKIPGEVISGVKNGLMGPLAGIGDTLDFGTFQTIFLALGASFGAEGSVIGAFF
                     PIMFSILLFCEGYYLFHLGYSLGRDSIKKILSGGIVNKIIDGASILGMFMMGALSATT
                     VKLSTPLSFDIGGKAIVVQDTLNMIAPGLLPLGVVFFVYWGMKYKKWTITKLLVILVV
                     LALVGSFIGIF"
     gene            complement(27865..28620)
                     /locus_tag="PC414_001852"
     CDS             complement(27865..28620)
                     /locus_tag="PC414_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2464249.1"
                     /translation="MESVLMLAIVTGLWYWFAAGLAGYTLFSTLKSPLFIGFSLGLLW
                     GDVTTGMIVGASIEMVYLGMVAAGGNIPSDKCLAALIAIPVALQTGVNAEVAVSIAVP
                     LGVIGVLVNNLRRTGNAVLVHKADKYAEEGNTKGIWRCATLYSLIFGFVLRFPIVFVC
                     NFFGADLVQSLLDVIPQWLMNGLTVMGGILPALGFATTIFTIGKNKFLPMFIIGFFMV
                     QYFEISITAAAIFGVCIALLITFMKEDKRVGEV"
     gene            complement(28647..29135)
                     /locus_tag="PC414_001853"
     CDS             complement(28647..29135)
                     /locus_tag="PC414_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2464250.1"
                     /translation="MAKITLVRVDHRLIHGQVITKWVKIAQAQKIIIVDDFLGQDEFM
                     ADIYKMAAPSGVEVVILTAEDAGQAFQNNTLGDKNIFILFKNVDMANKAYKAGLKYEK
                     IQLGGIPNEAGKKMVFTAVSLGNEDVEQLNELNENGVEIVLQVIPEESSMTYENALKK
                     FK"
     gene            complement(29153..29554)
                     /locus_tag="PC414_001854"
     CDS             complement(29153..29554)
                     /locus_tag="PC414_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HDF2464251.1"
                     /translation="MNKYKVIILCHGTWGKVLVEETNKNFGLSNQYEVLSLSSEKDVS
                     VFMKEVEEVVDKEDVKIIISDLYGGSTSSVAIAVAIRRGIDAINGLSLQTILIVDEEL
                     SKEDKLHTLPERIVKKNNNLCVDLIKEFKKI"
     gene            complement(29557..30054)
                     /locus_tag="PC414_001855"
     CDS             complement(29557..30054)
                     /locus_tag="PC414_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2464252.1"
                     /translation="MSLVKYCRCDDRLIHGQVIYKWVKHLGVKKIVVVDDETTNDVIA
                     KGLIKMAAPKNIDLSILTVSESRRYFYNNQADDNVFVLIKNLDTANRMIEEGINIKKL
                     IVGRIPTGIGKKKISQNVYINKKEFLLIDEFIKKNINVSIQMVPDEEEVELNQNINRY
                     KGEFI"
     gene            complement(30078..30482)
                     /locus_tag="PC414_001856"
     CDS             complement(30078..30482)
                     /locus_tag="PC414_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2464253.1"
                     /translation="MIGIIVMTHGSFSEEIIKSCELIAGPAERTAAIKLNRNDNIEDL
                     NKNFVEKLNELDEGDGVLVLADLLGGSPSNVASLNLKKGGKFHALTGVNLPMLLEALI
                     NREGKSLEELAEACIEAGQTGINNINKVLSSM"
     gene            complement(30712..33279)
                     /locus_tag="PC414_001857"
     CDS             complement(30712..33279)
                     /locus_tag="PC414_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2464254.1"
                     /translation="MTQKKLSRKEKVLICLKRLTEEHLNFNNGIRFGFDAEYIATEVG
                     INRSNASKELNQLIKESIIIKIKGKPVQYLYKESIESILGKKLNKGIFDNINSINEYS
                     ENDSIKDEYILESIKKENNIFNLVGCDGSLRSQIEQAKAAVIYPPKGLSTLIIGPTGV
                     GKSIFAEYMYKYSLTIKEDSDNAPFIIFNCADYSDNQQLLLAQLFGYVKGAFTGADKD
                     KYGIVHEANNGVLFLDEVHRLSAEGQEMLFLLMDKGIYRRLGEANKVHECRVMIIAAT
                     TEDPETAMLETFLRRIPVTIKIPSLEERGFQERMKLICYFFKEESVRIGVKLKVSKEV
                     IKAFILYKCKGNIGQLKSDIQLICARAFLDYMTYKRECVYVRLSLLPNNIRESLYGNN
                     RKEEFVQNFSFIGKDDIIFLPEEKDFESENLLIENKKYDLDFYGMIKETWNKLQQEGI
                     KESQIRSVIDGDIQKYSYNLMNTFIYNSSNQTAYNNIVNDSIATTVKYILQKHDKWSK
                     REGLDKLIKAIALHIQNLIERIKIGNIVKHPNKDEIMKERAYEYNIAKEILSNMSLMY
                     GVEFPEDEAIFLATFLYLSYVGLNEESIAILVIMHGESTASSMVNVANTLLDCNHAVA
                     INMGLEDRVQDILVQAVEIANKIDKGKGILILTDMGSILTFAKVIRDATGKEVKAIDM
                     VSTPIVIEATRKALTPEMTLEKLYFNIIESIKKHYGDMEVTYAEKDNGGRYFDRLLID
                     NISKTLTFLNGEKAYFILKEVINRISEHYSLVIQDELLVKFIFHCSCMIERVIIKESL
                     VYKSYEERISRNKELYLVIKESFKLVEETFDIIISDMEYANILDIFESQYDTDKFKS"
     gene            33445..34188
                     /locus_tag="PC414_001858"
     CDS             33445..34188
                     /locus_tag="PC414_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HDF2464255.1"
                     /translation="MNFLSDLHTHSIVSGHGYSTLLENINYCKENGIKILGTSEHGPK
                     MPGSPHRWYFHNIQNIPRIINNIIILRGCEANILDIKGNIDLEPFVIPRLDYLILSFH
                     EAVFSPNTLENNTKALINAINKHDNIEILGHLGNPNYPIDYELIIKLAIEKNILIEIN
                     NCSIKGVSRNGSSDNCKYIATLCKKYGAKIILTSDAHICFDIGNYEYSENILKEINFP
                     DELIMNYPKKLINHFHQKGKLLDVDYTNI"
     gene            complement(34500..34706)
                     /locus_tag="PC414_001859"
     CDS             complement(34500..34706)
                     /locus_tag="PC414_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464256.1"
                     /translation="MSKYSSLWEYVKKNNSQSFKLTFEEIKDIAGIEIDHSFLKYKKE
                     LNEYGYQVGKISLKEKTVIFDKIG"
BASE COUNT        11296 a         6379 c         3560 g        13638 t
ORIGIN      
        1 cttaaaaata attcgtcaat acttttttaa attatttttt aaaaatattt actttcttat
       61 attacataat ttattgaatg gcagtcatac tgttggttta gagtatttta acacacacat
      121 tttgttgtaa aaattacaac tattaatata atatatatac aatcataagt attataattt
      181 ttatgaatct aatttaaact taatataaac ttattaataa accttctatg tctaaatttt
      241 aaaattacaa tataaaagct ctacactaaa taaatacatt aaataaacat aatggataat
      301 gaatataaat ttatatatgc aaagcgctcc aataaaaagg taccaataaa gttaaaagaa
      361 acttattgat acctttcatt attacatact tcaatatata attataagtt ttttaaatag
      421 tatggattta catttatccc ttatctcata gctctcatag catttactac tgctataagt
      481 gccacaccta catcagcaaa tatagcttcc cacatagtag ctactcctcc agcacctagt
      541 atcataacta taatttttac tcctagagca aatattatgt tttgccacac tatcttatta
      601 gtcttgtttg ctatttcaat tgctttagat atcttgctag gctcatctgt cataagcact
      661 acatctgcag cttcaatagc agcatctgaa cctaagcctc ccatagctat accaacatca
      721 gcacgagcta atactggagc atcatttatt ccatcaccta caaatgctat tttctctttt
      781 tcagttcttc cttcatataa atcttctaat ctatctacct tttcattagg aagtaaattt
      841 gagtaaactg tatccaattc taattcttga gcaatatttt ttgctacttt ctcattatct
      901 ccagttaaca ttacaacttc ttttacccct atttctttta agcttcttat ggcattttta
      961 ctatcttctt ttacttcatc agagataact atataacctc tatatgcttt atcaacagca
     1021 atatagacta ctgtgcctac ttctttagct gatgaataag atatattttc ttttttcata
     1081 agtttctcat tacctgctaa tataaaattg ccattatgct ttactctaat tccataagct
     1141 gctatttcct cataactgtc tattgtatct aaatcaattt ttttattata gtaacttaat
     1201 atggactttg ctattggatg atttgaattt acctccgcaa tagctgcata ttctaataat
     1261 tcttcttctg atataccttc aggatttaac ttagttacat tgaacacacc tttagtaagt
     1321 gttcctgttt tatcaaatac tactgtatct acacttctta aagcttctaa atagttactt
     1381 cctttaataa ggataccatt tttagatgca aacccaattc cactaaagaa actaagtggt
     1441 attgataaaa ctaatgcaca agggcaagaa acgactaaga atatcaatcc tctatataac
     1501 caatcactaa atacctcacc tgagattaca agtggtggca caaatgctat taataaagca
     1561 gctattacta ctattggagt atagtatctt gaaaacttag atataaagtt ttctgtcttt
     1621 gattttttaa tgctcgaatt ttcaacaagg tcaagaatct tagaaactgt tgattctcca
     1681 aaactctttg taacttcaat actcaataaa gcatttttat ttataacacc tgataagatt
     1741 tcatctcctt tttcaacttc tctcagcact gactctcctg tgagtgcaga agtatctaac
     1801 atagaaactc catctactac tacaccatct aatggtactt tttcaccagg ttttactact
     1861 attatatcac ctacatttac ctcttctggt gacaccacct taacttcaga attaactttt
     1921 agatttgcat agtctggtct tatttgcata agtgaagtta tagactttct ggatttacca
     1981 actgcaacac cttgtaaata ctctcctact ttatagaaaa gcataacccc tactgcttct
     2041 gataactccc ctattgctaa tgctcctact gttgctagag ccatcaaaaa gttctcatca
     2101 aatactcttc cttttgatat atttcttatt gctcttaaaa gcacatcacc acctactatt
     2161 acataggcaa ctataaatac tatattgcta aactgacttt cataacctgt agctgtttga
     2221 tatataccaa atatatatac aagtgctcca attataagtg gaattaattc tttcttattt
     2281 gtatcattta cagctcctga tttttcagta gttctccctt ttactttgtc agtagaccct
     2341 acttgtatat caagccctgg ctctgttgaa tttattattt cttttatttt atttataaca
     2401 tcatcctctt caaagttatt acttatattt actgtaagtt ttttatttac aaagtttaaa
     2461 ttagaagatt caacttcttt taatttagaa actttgttat taatctcttc tgcacaatga
     2521 gcacagttta atcctcctaa tataagctct tttttaattg atgttttact agctgcattt
     2581 tctttagaac ttatttgtat atcaagacct ggctctgttg aatctattat atctattatc
     2641 ttttctatag tagtatcctc attaaaactt tcctttatat ttactgtaag ttttttattt
     2701 ataaaattta aattggaaga tttaacttct tgtagtttag aaactttatt attaatctcc
     2761 tctgcacagt gagcacaatt taatcctcct agtataaatt cttttttaat tgaattattt
     2821 gcttccatat atattcactc ctctcattta tcatttataa gtctctctaa tatgatttaa
     2881 tccacaatca aatatctgac ttatatgact atcatctaat gagtaataca ctgtttttcc
     2941 ttctcttcta aactttacta atctagcctg ctttaatact ctaagttgat gagatattgc
     3001 agaatgagtc atatttaata aactcgctat atcacataca cacatttcat ttgcaaataa
     3061 tgcatataat atctttactc tagtagtatc cccaaatact ttaaataatt ctgctaaatc
     3121 atataacatt tcttcatctg gcatagttga tttagcctct gttactattt cttcatgtac
     3181 tatattacaa ttacaatcat ttatctcttc tctcattttt aatcctccat tattttaata
     3241 tatgaataac tgttcatatg ttcattatat gttatcattt ctaaatttgc aacaataatt
     3301 tttattaatt taatataaat agaatgcaca tattataaac ttttttaata tatatattat
     3361 tagaataagt ttttgtaaac ttttttatag ttttctagga ggtgcaagtt taatatgagt
     3421 aacaagaaaa aaaaggattt agatactagt tacatgcctt acttagaccc agcgaactat
     3481 gcagattata ttactaatga agaaattccc caagatgaat tgagaaatga ccctcaaatt
     3541 tctaatggtt atacaaaaat acctaataac cagaacataa ataacctata tcctaataat
     3601 gtaaatcatt atcctaaaag tagtaattct ccaaacaact tgcctaacac aaataataca
     3661 tccggaaata tgaattacaa tacatctgct acaaataata tgcctaataa catgaataat
     3721 aatacgggta tgcctaataa tatgtctaac aatatgaata gtaatatggg tatgcctaac
     3781 aatatgccta ataacatgaa tagtaatatg ggtatgccta acaatatgcc taataatatg
     3841 aatagtaaca tgggcattcc taacaatgtt cctaataaca tgaatagtaa tatgggtatg
     3901 cctaacaata tgtctaataa tatgaatagt aatatgggta tgcctaacaa tatgtctggc
     3961 aatatgaata acaatatgta tggtaataca ggtatgccta ataacatgaa cagtaatatg
     4021 gggtataata tgaacaatac tatgggtatt cctaacttct catgtaatca aaatatgcct
     4081 cctaatgtac ttatgatgat accaggagtt atatgtcata atacaatgca aggtatgcct
     4141 gtagttatgc ctagtactat gcctcctaat atatatccta ctccttatgg ttctagtaat
     4201 atgtctatac aaggtatacc acaggctact aatattgagg aatttgatga ggaagaaatg
     4261 taatatatat ttttttaagt aagttgtata aaacattaat aattgaccat aataataaat
     4321 gtattcctca taatattccc ccaatattat tataggtata gaaagttgac tgaatatgcg
     4381 cttattcggt caacagatat taacataatc taataagata aacatagaaa aagtcagata
     4441 aaatttaatt ttatctgact ttttcattta aaaatgctct cttgtattgg tgggtcagaa
     4501 tagtatgata ctcgtatttg agactaatat tggcaaacaa cataaagaaa aatataaatt
     4561 ttttataata aactgtataa aattttaata attgaccaca ataataaatg tattcctcat
     4621 aatattcccc caatattata gaaataaatt taagcgagat taaattcagt taatctcgct
     4681 taaataatgt ctaaatttat tattccaaaa aatcatcaaa atctaatgaa cctgattctg
     4741 caaaatcata taatgcattt tgctcaaaga aaaatttctc ttcaaattta atatagtcgc
     4801 tataattaga ttgacttatt tcattgatat aattgcaatc attttcgtta taagtaccaa
     4861 taaaaaactt atttcccatt aagtaatata tagctttcat atcattattt attatatata
     4921 cattttcttt tacatggtca ttattaaaat taaatctagt aaagaatata ccttcattca
     4981 tattactttt taacagaaat ggattttctc tataaaatac tcctgcaaat tcttcaggtg
     5041 aatttacact gtaaacactt acaaattcac ttttagaaat ctcatcaaat acctcaaaat
     5101 catacatagg ttctttatct gtcactaata gagaattaat tctaaaacca gtttcataac
     5161 acttacttat gcaaaaagca attttacata tataatatcc atcattatct tcatataaag
     5221 cttcacttat atatctgcct tgacctttat cacttaccac gtttttaagt aatgttccat
     5281 ttatattagt aatgtgcata tttgcaatct cgtcacttcc agaaaagtac tttaaacttt
     5341 ctctatccca tgctataaat ctcatggtca tatagtttat aaattcaact tcctcgtcaa
     5401 ctcttttaca tattttattg tataattttt ctatagttaa atctgtttta tagtttttta
     5461 taatatcaat atactcacat ttatctcctg gcaatagctt atcataataa agattcttat
     5521 taccaaaata agatactaaa aataaagatt catccttcgt taattcaact ctatctgaac
     5581 caaatcctcc catcaatctt tcttcttcca tataagcttc ttcttgtgtt gggttattta
     5641 aactcacata atccgctaat cctaatccct ctgcatctaa taaaaaatat tgacaaaagt
     5701 gattgttttc atcatctatc caatttatta taagacctaa actgcccata agtctagaat
     5761 ttgtagcctt tgcaaaatta aaattcatat agtcacatcc tttaaactct aaaatacttg
     5821 ttttttaata tataaattat tatacatata ttttaacaac ttagatacaa tttataatat
     5881 aatataagga aatgattaat tgaggtggtc ttatgaaaaa tatagcagct ttttttgata
     5941 tagatggtac tctttataga gactctctta tggttgaaca ttttaaaaaa ttaattaaat
     6001 acgatataat tgaccaaaaa aattggtata aacatgctag agacactttt atggattggg
     6061 ataaaagaca aggtaattat gatgattatc tattagaaat atgtgacctt tatgttgatt
     6121 cactaaaagg attagataaa acctgtatca actttacaag tgaccaagta attaaattaa
     6181 aagcagatag agtttataaa tacacacgtt caaggattga gtggcatcta aatcaaggac
     6241 acatagttat atttatatct ggtagccctg gatttcttgt tgaaaaaatg gccaaaaaat
     6301 ataatgtaac cgatttttta ggaagtgatt atgtctttga aaacaatata tttacaggca
     6361 cagttattcc aatgtgggat tctataagta agaataatgc tataaacgat tttgttgtaa
     6421 agtatgattt agatttatca aactcgtatg cctatggaga tactaatggt gatataaata
     6481 tgctaaaaag agttggaaat ccaatagcaa taaatccaac aaaggaactt ttatctcaaa
     6541 tatcacttga tgaggaaatt tctaaaaatg ttgagataat agtagaaaga aaagatatta
     6601 tttattctct atctacagat gtaaatatag tggatattta atttatacta tctattttaa
     6661 aaacttatat tatatctaat atttatatct aacatataaa agctttgact aaaaatcaaa
     6721 gcttttatat gttgcactaa gagaatatat taaatttagc tttatgcttt aatgtatatt
     6781 catatatatc atttacactt atctaaacta caatttcatt aaaattaaat ataaaaatca
     6841 actattattt tttaaatagc atctttctta atctattatt gtaataacct ataaaagaac
     6901 tgtaaacata gtcatatact ataaaggcta tctcaaatga acctataata aatatgttta
     6961 ttggaatata aactattgtt cttaataaaa aatacaatat aagtatcatt atatttgcaa
     7021 atactaattt cataaaatac tcaatatata tagacctatc tttttctata agatatttta
     7081 caataccata tattccaaag gtaagtgaat atacaatcca ttgagactta cttgccatca
     7141 ctataaatcc taaaactata gaacctatat aaaaagcaat tccactctta aagccccatt
     7201 ccattattac aattgatact aaaagtgatg ctaaccccat aataaacaat gtatttacag
     7261 gaataatatt tgtaagagtc aacaatatca catttaatgc taataaaact cctccataag
     7321 caacttttat actcatattt atcatcctaa caacaagtta ttaaatctcc accaaaacat
     7381 tcacaacatg aatctaaaca ccaaagctta gcacaatttt cacaacaatc gtcacctaga
     7441 caacaacagc aatcatcaca gcaacagcaa caacatccat ctaaatcatt acgtcttctt
     7501 ctattataat tgtcagccct attgctataa tcatttctat attgtgtata acttctaagt
     7561 gcatcactat attcactatt ttcaggctcc ataaattttg ctctttttaa gaagtcttca
     7621 ccttcttcat aatatcctat gttcattgca gaaaagccag ttaaataata ccactctgca
     7681 catttgtcag tcaaagtttt taaaaaatca tatgcttttt taaattcact attttctatg
     7741 aatgctcttg ctctcataaa attttcatta ttataagtat tactgtacat tctttccacc
     7801 tctactttct aatatattaa tatatcttaa ataaacacca gaataaatta tattttctat
     7861 tattccaaca tttttattta gatttagatt atcaattccc ctagctaaaa accctaatga
     7921 tgttactatt atttttttta ctttttcttt taactcatca tttttatcaa tatactctat
     7981 aaatggatta tatcttcctt tcttaaaatc ttcatctaaa tcttcatagg catctagtag
     8041 atatatatac ttacctatat taaaacctat cattcttaag tttctctcat tttcatcttt
     8101 tttatagaca aatacttcac ccattatatt tccaaatata ctagatactt catctatatt
     8161 tatattttta tctttttcta acttatttaa ttcatttagt tgttgtttta tatactctgc
     8221 tttttcagga tacttttcat atgctaattt taatttgttt ttatatatat aatatgccaa
     8281 tttatctttt atgcgtttat catccattaa attatcttcc aatttataat acgttaaaag
     8341 aatattcata cttgctgcat attctgtaat atcatttgta attacctttt tctttttaaa
     8401 tggatttact atacacactt cctctgtact tatagactct gggttatata ctgctgttaa
     8461 taaaacaatt aaaaatgtta tatcataatt caaagaaaac ctagagattt ctccatgatt
     8521 tctttttaaa tatttacata gtccacaata gtaagctttg taataatcgt attctctaaa
     8581 tgttaaatcc attttgttta ttttaacata tccaaacata aatactcctt tttgtactat
     8641 tttttattta gattatatat agtataacac tttttatcaa gattatataa tttacagtat
     8701 tctaagaaac ttaaaaaatt ccttctaatc attgtatctt aaatttaaaa taattagaag
     8761 gttgggttaa aaataatatt aaattatata cttatattaa ctcatacaag ttagttgtgc
     8821 ttatatcaat ttaagtttta aataattggt atatatttaa aactacctac agtatccttc
     8881 aattcttttt ttgctttatt tataaccaat tcatcaagca ttacatcatt tattgaacat
     8941 gatatagatt tagatgccaa taacattgct ttaaatccca tatcagaacc tgcactggca
     9001 caagattgcc atgtatgggc agaaatacca ataggccaaa ctgaacaaga acactgtgct
     9061 gtaggaatta tgtaactaac atctccaata tccaatgata atgatataca gttattatga
     9121 gtaaatgtat catccgtaac ttcatcatgc agattcatct tagtaatatc cttatttcca
     9181 cttaatacac tagaaacact acttctttta tctatagaca ctgtctcagc taatttatct
     9241 gcaaaatagt agtcatcttc attatagttt tgtacaccta ctaatttcat attttgtgat
     9301 agaatatctg ttaatattgt atttggaata tagtcatata ctccacttat aatgtcatat
     9361 tccataatag taccagtcat taatgctgct ccttttgcta tatcaacaat cctaccaaat
     9421 acatctctta catgctccat tttaactcct cttatgtaat accacacttc tgcaaaatca
     9481 ggaactatat ttggcatttt tccaccatta gtagttacat agtgtactct acaattatca
     9541 aatatatgtt ctctcaaata attacaacct atattcataa gttctactgc atcaagtgca
     9601 cttcttccgt tataaggagc ttgtgctgca tgggcactaa tcccattata tctaaatttc
     9661 acagagtaat tagcaagtgt tgggattctt ataggagtat ttatatcaaa tggatgccaa
     9721 ctaaaagcac aatcaattcc atcaaaaaaa ccttttttta tcatcaatac ttttccagat
     9781 aaatcttcct ctgctggaca tccatagtat tttatagtcc ctttcagctt ttttagtttg
     9841 attaattcct ttactgcaat tacagctcct attgaacctg tacccagtaa gttatgtcca
     9901 catccatgac ctgcaccacc atctacaata ggttctttaa tagtggatac tttttgagac
     9961 aaactatcta aagcatcata ttctcccaaa actgctataa ctggtctacc ttctccatag
    10021 ctagccgcaa atgctgtatc cataccttct aattcatcta tactaaaacc atattttttt
    10081 aagtattctt tttgtaaatt tgaagcataa tattctttat actgaagttc tggatttttc
    10141 catatcttat ctgatatatc tcttaattct ccctgtatct catctatata ttccatcaaa
    10201 aactttttat ttaaattctc catattaatt atataaacct ccctgtttct aataacttat
    10261 cgattatcag ccaaaatgac taattttaac tgatttaaaa atattttcta tcatacaacc
    10321 attttataca ataaaacctc ttgacttgta agtttcattt tatcaagtaa gaggttttca
    10381 agttttataa aatttttagc atgttaacca atcaagcata ttaacccata atttattata
    10441 gtatttccaa tcagtaaatt cttttggacc ccagtgaggt gcacagtcag aactaaagat
    10501 agcagatttt ccttttccaa actcgccaac agctacaaat gggtctcctc caatagttgc
    10561 taaaacagga caattatctc tagctactgt tttgttatat cctaaaaact ttggccattc
    10621 tgttggtata ccttttaata ctggatgttt agagtcaata acttctggaa ttattcctgc
    10681 tggtttttca actctatcat ctttatctat cattgttatt ggtaatacat ctttgatagc
    10741 agtttctccg aatctagctt ttgcatctat tcctgtaaat gacatatatc cacctaccat
    10801 gattaacgcc ccaccattat ttacatactc tttgattagt tcaagtctat ctggattact
    10861 attgcaatct ataaaagtac tattagataa taagaacgta ttagaaccta tatcagataa
    10921 tattatacag tcatactcat ttaactcttc taatttatac ggaaaactat ctgctgctac
    10981 atgtgcaggc atatgaactg tctcatatcc tccagattct attgcttctt tcaaccactt
    11041 tacagcttct tcataatgag ttgtaacaaa cgtatcaaat ccttttatat gagttatatt
    11101 ttttacccaa gattccccta ctattaatac ttttttcatt tatatattcc tcctactttt
    11161 ataagtttaa tattagactt gcatacataa gtatactatc taaatactcc ttaatttcaa
    11221 catattcatc aattgaatga cattgttcta ttgaacctgg ccctaatata agtgttggta
    11281 tttcagctaa atttttcatt aatctcgcat cattgccagc tgttgaacct tttatctcta
    11341 attttttccc tagagtttct tctagtattt tatgtgcatt cactacaaaa tcagaatcct
    11401 tgtccatctc aaaacctaac cctgattgat atatgtttat atttggttta ttatctttaa
    11461 gccataaatc tccattagcc cttgtcatta tgacatttgt tacatcattt acaacttgtt
    11521 cataactcat tatatttggc aagaagtgta cacataaatt aaatacacac ttatctggca
    11581 cagtagaacc tgctgttcca ccatttatta cacctaaatt tattgtaggt gaaggtaata
    11641 gtggatgttt atatatcata agccagttat gttccaactc ttttatatct tgaagcaaca
    11701 acatagcctt ctcaattgca tttacaccct cccacttact accacagtga agagacacac
    11761 ctttaacctc aacttcaaag aatacaaatc ccatatgtgc aactattaag ttttgttctg
    11821 atggttcaca aattatgcaa caatctccat caataccatt cataactgca tttatggtac
    11881 catttccgcc accttcttca tctactactg acattatttt tacattacca ggtacattta
    11941 gaccactatc ttttataagc tttacagcta atattgatgc tataagtcca gatttcatat
    12001 cagcagttcc aagtccataa agttttccat tatcttctgt agcacgatat ggattatatt
    12061 tccacttact tatatcacct ggtggcatag tgtctacatg accattaaat acaattgtct
    12121 taccaggtaa atcatcacta tacttacaaa ttaaattata tctatcttga taattgtgac
    12181 ctagatttcc ttctttatat atattttttg cttttttaat taattcttcg ctcatttctt
    12241 gtctatctac actaaaacct atgtaatttg caagtttttc aatatattcc tgtccttctt
    12301 tttcaaatcc acctagtatt ccatgaccaa catcttcagt ctttatactt acaagttctt
    12361 ttaaataatc tatgtattct tgttttttac tatttaatgt atctaagaga atttttttca
    12421 tatttcctcc taagatttat ttttacattt aaaatttcaa gtctaatctc atctatttaa
    12481 gccatagtaa actttttcac ttagcatatt cccattcaat gtataaagat gtatgcaaat
    12541 taaagtataa aaatatatac aaaaacttaa taatttttat agtttataag ctaaaattta
    12601 attactattt agatttagct gattttttta attttatatt atttatccat atagcaaata
    12661 acattaccaa accttttata atcatttgat tatatgaaga tacacttaat aagtttaatc
    12721 catttcctaa tattcctatt attccgacac ctatacatgt acctactata gaaccagaac
    12781 cacccatcat attagtacca ccaagcacaa cggctgctat agcatccaat tctgctccat
    12841 ctccaacagt tggtgaacct gatgccaaac gtgatgatag tattatccca gctaatgctg
    12901 ttagtagtcc agctagtgcg tatactttta ctataacttt atttactttt actccacata
    12961 atctagcggc ctcttcattt ccacctactg catgtacatg acgtccaaaa ttagttcttt
    13021 ttaatagaaa atgtcctgct attacaacta taatcataat tataactggt attggtattg
    13081 gtccaatata tcctctaccg agcatcttaa atgcatctgt atttactgat ataggtcttc
    13141 ctcctgtaaa tacaaagctt gccccactta ttatcgttaa catggctaaa gttacaatga
    13201 atggaggtag tttttgtgtt gaaataattg caccttttat gactcctaaa attgtcccta
    13261 ttgctaatgc aagtatcata cctacccata taggtgcatt ataattcact actgttgtag
    13321 cccaaaatgt acctactaaa gcaattactg ctcctacact taaatctatt cctcctgtaa
    13381 ttattacaaa tgtcatacct actgcaagta ttgcatttat tgatatttgt cttccaacat
    13441 taattaaatt atctaaagtt aaaaagttag atgaagctac actcattatt ataaaaatta
    13501 ttacaagtcc tacagctgca ctcccttggt ctctaaatac acttgttaat tttgtcaata
    13561 atggagaaga tttttcttct ttatgcttag ttattgaact cattaatttt acctccgaaa
    13621 gccaatttta atacattatt ttcaaccatt tcttcacctt ctaaaaatcc acttatatga
    13681 ccttctctca taaccattat tctgtcactc attgatatta attctggtat ttcagaagat
    13741 atcattatta tacttacacc actctccact aaatctccca ttagattgta tatctctgcc
    13801 ttagcattta catctatacc tctggttggt tcatccataa tcaatatatc tggttgtaat
    13861 aaaagccatc ttgctattat agctttttgt tggtttccac cacttaaact ttgtataact
    13921 tgattcatat gtggtgtttt tatctttaaa gcttctatgt atttttctaa aatctctttt
    13981 tccttttttc tatctataat tgatttaaac ttagatatct tttttaaccc agccatagtt
    14041 gtattttctt tgacactcat ttgtaaaaac aatccagtac cttttctatc ttctgttatt
    14101 aatcctagtc cattttctat tgcatctgat ggagttttaa actttacttc ttcattgttt
    14161 aaatatattt ttccagaatc atatgttcta agtccaaata tagcttctac tatttcactt
    14221 ctcttacttc caactagtcc acctattccc aatatttcac cttttcttat attaaagcta
    14281 atatcattaa aaacattgtt cttagttaga ttttccactc gcataatttc ttcttttatt
    14341 tctacttttc tttttgggaa aaattggtct agctttctat ctatcattaa attaactaat
    14401 tcttcctcag acgatacatc tttggtattt aaagtcgaaa tcgttttccc atctcgcatt
    14461 atagttattc tgtcacaaat ctcaaagagt tcttccatcc tatgggatat atatattata
    14521 ctaactccac tctcttttaa atcatttata agtctaaaaa gtatttcagt ctctttttta
    14581 cttattgaag aagtaggttc atccattatt aatactttag tttctgatga taaaactctt
    14641 gctatttcta ccatttgttt ttgagctata ctaagctctt tgactaaact atttggattt
    14701 atatctattc ccatattatc gaaaatcttt ttagcttctt cattcatttt tttgtcattg
    14761 gttaagactt tgccatttat ttcttttccc ataaaaatat tttgtgctac tgttaattcg
    14821 tcaaaaactg ttagttcttg atatatggca tttataccta tctttttaga gatatctggg
    14881 gatatattct ctatttcttt gccttcaaaa ataaattttc cagaagtttt cgaatgtgcc
    14941 ccagttaaaa tctttatcat tgttgattta ccagcaccat tttcacccag tagtgcatga
    15001 acttcgccat gaaataactc aaaattaaca ttatctaatg ctctgacacc aggaaattct
    15061 ttagcaatat tacttaactt cagaattata ttactcataa tgacactcct ttttaaatag
    15121 gatttctttc aaataatctc attttatttt actaattctt aaagtctttt atattttcct
    15181 tatcataaag taaagctggt actggtatat tcttttctac cttttcccct tttatagctt
    15241 ttactactgc ttctacagct aaacgtccca tctctttagg ttgttgttga atcatagcaa
    15301 gaatagttcc atctgttatt ccacttgatg cttcttcagt taagtcaaat cctacaatct
    15361 taacatcctt attttttgca gattcaagtg cagctttagc tcctagtaca ctattttcag
    15421 ctgttgcata tattatatct acatctggat tagattgaat taagttttct actgtattaa
    15481 gagattcttc tctgtcatat cctggttgtg aatttaatat cttaacattt gctgaacctt
    15541 ttaatgcatc tttaaatcca tctattctct gcatttgtat ttgagatttt aaatctgtca
    15601 ctattgctat ttctgacttt ccacctaact tttcatctat gtatttttta gtgtattccc
    15661 ctaatttttt accagcatca taactaactg ttcctacata acttgtaata ttttcagtat
    15721 ttaatacagc atctactgta actactggca ttttttcaaa tgctccttct actgctggta
    15781 ctatagccga attatcagtt ccaagtacta tcattccatc tacattctgt tgtataaaat
    15841 cttctattgc tgatgtttgc ttagctacat cttggtctgg gtcatttact ataactttca
    15901 cacctaattc tttagctttt tcttttattc cctcatcaat atctataaaa aattgatgtt
    15961 gaagagttat tgtagatact ccaatagtta aatctttctt gtctgaacca cctttattat
    16021 ctgatttact tggtccttcc attgaacacc ctgttaacat tcctattgct agtattccag
    16081 caattcctaa agataaaatt tttctcactc tcataattaa cctccatttt taatagtttt
    16141 aaatttgaac aaattagatt tttatttatt atttaaatac ttttcaacat cttctttata
    16201 gggcatacca gattgtgcac ctagtttagt tacagaaaga cctgatacta ctactgcata
    16261 ttctactgct gtatataaat ttttcccttc tgttatagcc actgccaatg ctccattaaa
    16321 tgtatctcct gctcctgtgg tatcttgaac atcaacactt atacaagata catgcttgat
    16381 ttcctcacca tcataaaatc tagctccatt ttttccttct gttattacaa gtttatttgg
    16441 atattttttt aatacttctt ctatcccttc atttgtgtca aaaactatct tatattcatg
    16501 ttcatttggt gttaagtaag ttactttttc tattatatct tcatttagtt ttactgctgg
    16561 tgctggattt aataaaaccc taatcctatt ttcaaaacag aaattcacta cataatttat
    16621 tgtttttaat ggaatctcta attgtagcaa tactatatct gcattcttta tctcttcttc
    16681 attcttttta attaattcta tatctactag ctcattagtc cctggtacaa caacaatact
    16741 attatcattt tcacataatg ttataacagc aacaccagaa cttttgtgat tatttctgga
    16801 tactaagcta taatcaactt tattcgttct aaaatttctt attaactcct ctcctaagcc
    16861 atcttcccct atgcatgata tcatttttac atttgctcct agtttagaag ctgagatagc
    16921 ttgattagcc ccttttcctc caggtgatgt aaaaaaacta ttacctaata ctgtttctcc
    16981 tttttctggt cttttatcta cagaacatac catgtccatg tttacacttc ctatgacaac
    17041 tatatttccc attacattac tcctttacat atctatttat gtctctcgtg ctgttattca
    17101 ctttcttttg ttgaatctct tatgaccaat tctggtttga aaatcaattc atcaaacgat
    17161 tcttttttat ctcctcttaa acaatttatc aatatttcac tggctttata tcctatatca
    17221 taagacggct gttttactgt ggtaagagaa gggcttatta gacttgatgt ttgaatatca
    17281 tcaaatccca cgacacttat atcatttggt atacttaatc ctctttcttt aagacctctt
    17341 atgacaccta ttgcaatcaa atcatttgca caaaatattg catcaaaatc tatcttttct
    17401 aaatttttta taaattcttg tccccattca atcttatatt ttccttcaaa tacataagca
    17461 ggattgtagc ttaaattaca ctcgattaga gcctttttat atccttctaa tctatctact
    17521 gctatttcat tttgaagtgg accacttagg tatattattt tcttatttcc aatatctaat
    17581 aaatgttttg tagctatata ggccccttca aagttattta ttttaacaat tccttttaag
    17641 gtttctttgt tcatacttat cttcttatca atcaagacag ttggtatttt tatatcttta
    17701 taatttgctt ctttccagtt tgtatttgaa gatgctgcaa atataattcc atctatcatc
    17761 ctctctgata gtgttcttat tccttcatat tcttttttag catcttcatc tgtattacat
    17821 aaaattatat tgtaaccttc ttcattagct ttgtcttcta cacctcttga tacatctgta
    17881 aagaatggat ttcgtatatc tggtataaga agccctatag tattagtttt tttagttact
    17941 aaactttgtg ctaatttatt tggtacataa tttttttcct tcattatttt caaaattttt
    18001 tctcttgttg cctgacttat attttcatct ttattattaa tgacttttga cacagttgtt
    18061 ttagaaacac cagccaattc gccaatttct tttatagtta tactcaagaa gttcaccccc
    18121 aaaatttagg aaaccgtttt acttaaccgg ttacctagat actatcacat cttttttcca
    18181 tttgcaacca ttttatataa attttgatat ttttcccttt tttcaaaata ttgcaactta
    18241 taattcaatt ttaatattta aattaatctg caaaaaaaaa ttaatatact tttataatta
    18301 tctaaaagta tattaattaa tttaagcagt atttattaaa gttcaaatcc ttgctctata
    18361 atcatttttt tatcattttc aatcccactt ccaacagttg tcaaataatc accaacaatt
    18421 gcagaattta ctcccccatt aaatccaact ttgtcatatc cctttaatga tagccttcca
    18481 ccagcatacc ttatgtacac attaggaatt ataaacctaa atactgctgt tgtttttagt
    18541 atttccattg gttctaatac atcataattt tccattggtg tattcttaat aggatttaaa
    18601 atgtttattg gaaatgattt tacatttaat tctcgtattt caaaagccat ttttagtctc
    18661 tgttcttcac tctcattcat tccaattata ccacctacac atacatctag cccagctttt
    18721 ttagcatctt ttatagtttt tatcctatct ttatatgtgt gtgttgtaca tatattatga
    18781 aaattatctc tacacgtctc aacattgtgg tgatatgtag tcacacctgc tgacttcaac
    18841 attttagctt gctcataatc tataatacct aaagatgcac ataattttaa attagtattt
    18901 tccctcaatc cttcatagat atttaaaata ttattaaact cctttcctga cataccttta
    18961 ccacttgtaa ccaatgagaa tctatgcact cctttactct ccatctcttt agctctattt
    19021 aagatttcat tgtaatctaa taatgaatat tcttctacac cagttttata atgagatgat
    19081 tgagcacaaa atttacagtc ttcactacat ttgccagatt tagcattcat tatcgtacat
    19141 aaatctgctt ttcttcccat aaaatattct cttatttcat ttgcagattt taatagaata
    19201 tcaaaatcgt ttttattatt tgtatcaagg cttattaaat taagagcttc ttcataactt
    19261 atttcttttt ctctcaatac tttatttttt aattttatta tgtatttttc catatatcct
    19321 cctatttgtc aacttaatat ttttatgtag tttacttatt atatatagta ttgcatatat
    19381 aacatttatt ggtcaacttt attttagaca caagtttact atacacaaaa aaatactgct
    19441 tcaaaataat attagtaaat ggattataca tattattatt aaaacagtat tttttattag
    19501 aatataattc ttactggttt ataaaactat ccttgtctta actccatcca agctttccat
    19561 tgtaataacc tctaagcctt catattctct aaatagccat tttaaatcct tcatggcttg
    19621 attaacctga ctttttgtta gattatttat aaaatattct tttctagttt cctcatactc
    19681 tttgctagtc aaaaagtttc ttagaaaatt aaacgcaaat acacttggaa tattgtttac
    19741 attagccttc ttgctgtttt ccttctcata gtaaattttc ataaatgcat actcctttga
    19801 tacttttaga tttctcttac aataattata tgcatttaag tgctactatt cttataattt
    19861 tattaattca tattctttac ttcccatacc catttcacat gcatagtcta ttattctagt
    19921 tccatttaca tgaggatgtt catgtttgaa aacatcatga cccatttcct tacaaatcaa
    19981 atcatagcat gcttgttcaa tagctacagg gtctgttgat gctaatattc ctatatcatg
    20041 ggcaattggt ctacctgacc aaggtacaca atcacaaagt ggagttacat tcataacaaa
    20101 agttatatac cctaccttgt cttttttatt tgatactgct ccataagcgt attctgccat
    20161 cttttcgaca aacacatctg agtcactttc ccattgtata gttactgctc tagttggaca
    20221 gacagttgga cattctccac atccataaca tacatcagaa tctataactg cttttttatc
    20281 cactatactt atagcttttg taggacaaga attaacacac tttccacaac ctacacactt
    20341 cttttccttt actactggag taacatcaga atgttgcatt tgttttcctg ctgcacttgc
    20401 acatcccata gctaagtttt ttaatgctcc tccaaaacct gctgcttcat gacctttaaa
    20461 atgactcatt actatcattg cagaagaatt ataaatttct ccacctattt ttacactttc
    20521 aaaatgtttt ttgtctattt ttacattctc ataatttcta ctatataatc catctgcaat
    20581 tattactggt gcatttacaa ctgcataagc aaatccattt tctatagcag ttgtaagatg
    20641 gtctacagaa tttgttctac tccctgtata cagtgtattt gtatctgtta aaaatggctt
    20701 tccttcgcag tctttaacct tatcaaccac ttgtctaact gccacaggac tcatatatgt
    20761 agtatttccc ttttctccaa agtgaagttt aattgcaact tggtcattct tttctattaa
    20821 atctttgaat cctgcttcat caaataatct tcttacattg tttggtacgt ttttattttg
    20881 cgaatttgag tataaatcac aaaaatatac ttttgacttt tccatatcta caccccttta
    20941 aaatatttac ttatactaat tttatcactt ttaaagtaat ttttagtata ttttaacata
    21001 attaaatatt cttatattta tttgatacaa ataatattat ttgtattatt ttctacactt
    21061 aattctatat attaaaaatc aaaaagactt gaaacttaag tccaagtctt tttgattttt
    21121 aggtgtagtt tttaacaaca taagattact ttaccttgtg ataaaggtat tattaaactg
    21181 gttttttatt aaataaaata acagctgcta gtaaacatac tacagaaata agaactgtta
    21241 taataattgg aataataaat tctggcattg ccactttccc tgaaatcaag tctatatttt
    21301 ttgacatcaa gaaattagga gtataaggtg caatctgttt aggaatagaa attaaactca
    21361 ataaaagagt tgccaccaat acaagtaaaa cactggcaaa tgctggtcta aatagtacac
    21421 acccaagaat taatacacta atatacataa ttcctataag ccataaatta aatgctgaaa
    21481 atataatatg attaagattt gcagttggcc aaaaatatat tgtatagcca taagcaaata
    21541 aaaaactcaa ccaaaaacca attgtcataa taatcaccgt aacagagaat ttagacaata
    21601 ctatagatga acgtgataaa cctttagtca gcataataac aagtgtccct ttagaatatt
    21661 catttgataa aatattacaa aaaataatta gagttaaact cataccaaga ccagagatat
    21721 tgctaaaaaa ttgtgtccaa gaatctaaag cagttggtgc agcagtaact tttaaagtcg
    21781 gtgcaaaatg agcaatcaag tctggcataa attttgcaga aataggacta cttataccaa
    21841 atattagaaa taaaataatt aaactaaata atttataatt tcttaaattc tctttaaatt
    21901 ctttttttgt aaatgctatg tattctctca ctttactacc tccaaaaata aattttcaag
    21961 tgataattct attagttcca tctttactgg acaaagattc atttctgcga gtgtagatat
    22021 aactgttttt tgtattgctt ttatatcttt accatgcaaa acaatttcca tattagtttc
    22081 ttctgaatct tttagtaagg atttaatacc atcactagac ttaaactttt gaatttcatc
    22141 attacttgca aactccagta aaagtctatc ctttccgtgc atacttttta tttctgaaag
    22201 tgtaccacta agggcaatgc ttcctttgtt taatatagca acatggtcac agattctttc
    22261 aacatctgat agtatatgtg ttgaaaatat tacagttgtt gagtctttga ttttaagcat
    22321 aatatctaat atttcttttc gaccaacagg gtcaagtgca cttgttggtt catcacaaat
    22381 aagtaatttg gggcgtgaaa gcaaagcttg agctatacca agtcgttgct tcattccacg
    22441 agaaaaccca cctattcgtc tcttttcatt tccaagaccc acaagtgata acaactcttc
    22501 actccttatc tgtatttctt tttttgaaag tcccgtaatt tcaccacaaa gagaaagata
    22561 ttcaagtggt ctcatatagt tgtaaaactc tggaacatca ggcagataac ctacatgacg
    22621 attagaactt gttttcccat aggtcacttt ctcaccacaa acatctatag ttccactatc
    22681 tggtttaagt aacccaagta ccattttcat tgttgtagtc ttacctgcac cattcttacc
    22741 tacaaaccca aagactgacc cttctggtac agtaaaattt aaattatcta ttatctttcg
    22801 attaccaaaa ccttttgata atccatttat tgagacaata ttcatcattc ttcacctctt
    22861 cctattgtaa aatacaaaat tggtcctatt agactaataa acagtactac aataatccat
    22921 attgccttat ttccaatttt ataatttgga tgtctcaata catgaacaag tgcagtaatt
    22981 ataagtatta aatctaaaat aataacagga accaaaagtg gtaagtattc catcaaattt
    23041 tccataatct tattcctcca ataaagataa tttttctata agaaatttgt attctttgtt
    23101 ttcacttaat acagaaaaca ttggattatt tttaatagca gcaacaatac ctgcttttac
    23161 gattgtatca tcacgtgtaa tacctgttcc aatttcagaa atccatctgt caagtttatt
    23221 aaaaaagtca tccccatgta ttataattgg gtatatatca cgtgttgcta aatctacata
    23281 ttttcgtaat gatttaatag ctccttcatt atcaccatgt attaaattaa tttgtgcctg
    23341 tgttaaatat gaagaaagca taatagcagg agataatgta tcaagctcaa atatatctga
    23401 aattgctaga atacgtcttt ctatttcctt catttgtaat ttatctgcat tttgcaaaat
    23461 acccactagg ttttggacta atgatataag gttctgatat gcaccaagtt gaaatgtttc
    23521 tctcgcttca tccatttgac cattcatcat ttgcccctgt gcaagaagta tagattcatt
    23581 tatcatggga aaattgctgt tttgcaataa atcaatcact tgtatgggtt gatttaacaa
    23641 tatataacaa gttgcctcca tactttttgc ttgacggcaa atatctatgt cattacaagt
    23701 ttcctgtata cgaataaata tttctaatgc ttcactaatt aatagttcac gctttttttc
    23761 atctacaata tcatagtgat taactattaa aagacccatc cgaaaaatca aaggaaaaca
    23821 tgaataatat ttttttatga tttctcttat ttcatccatg acttcatcaa atggtttaac
    23881 cgtaaagtct ttgcacagtt tattatacag tttattaata tcttccttca tcatttgagg
    23941 ttcataacat attaactcat caacggtaat gttgaaatat gtcgcgagtt gcggtaaaaa
    24001 agttatgtca ggatagcttt gtccagtttc ccatttagat acagaagctt tagaaacgcc
    24061 tatataattt gcaagttcat cttgtgtaat tcctttttct ttacgcttta aaatcagcgt
    24121 ttttgcgatg tttaattcat tcatgtcaat ccctccttat gtataattat atccttaaac
    24181 atgtaaagct aacaagcgat ttgaagttga cttttgacga aaaaatcaac cacagagaaa
    24241 caaataaaca agtatataaa atatatattt aaatttatct ttaatctctt ttgtaaaata
    24301 tgtaaacttt gtaaataaaa tccatattat ataaaactaa tagttaatct atctaaatac
    24361 agtttttatt tatcacgttt ttaaatattt tgtgtaaaac ctcttttaat ataaacacct
    24421 tattttcatt tctcatttca tattcttttt aaaatactga atataactct tcgattttcc
    24481 atttaattct tttataaagt tattattcgt atttatttct ctaattataa caggcaacta
    24541 tcatgtattg gtatttgtct attatataaa aatctataaa agaaaagcag tcaatcattt
    24601 agattaactg cttgtacata tatcttatta aactgtataa actataattt ttattattct
    24661 ccattttcat cccaagtcat aaatccccat ccactatctg gataagtatc aatagacttt
    24721 tctctaatca tataaccaac tttataacac tcttttgtta ttttattaga tagtatacat
    24781 ccttctccat ttatctaatt ccattcaatt aacttttctt ttcaacctta acttttataa
    24841 aatcatcttt tttaataatt tcatacttat tttctcttca acatcatcaa atgagggtgt
    24901 ctcaaaatta actttttact tcataaggca ctcttttttg tatgaagtaa aatatatttt
    24961 catcatttca acaataagta agaggcttat ctctattttg agacagcccc atttgatggt
    25021 gttgaacatt taaataaggt tgaactcgtt ttatctctct ttttaagata tctgtaaatt
    25081 tgcacataaa gtctcttctt cttttacaat tctaatagtt ttattaaagc caaagtatat
    25141 atctccatat tctttcgtat atgatttatt ggtactcttt catctactcc atgaccacca
    25201 gttttatacg ctaagaactt aggtccaaac gccaccatat ctttaaacca acttgcatat
    25261 gtgccaccac ccatagcaat tgtatatcca ttttcatctg ttacttcttc ataggcttct
    25321 aataattttt taagtactaa attactctca tccacataat gtaagtcttt atgagcaata
    25381 acctctattt tccaaccatt acaaaaaacc tttttgaatc tatctattat attgtttaaa
    25441 actatatttt taccatacct tatatctaac tcacatacga ttttttcctc atttactttt
    25501 aatattccta aatttaaggt agtgtttttg aatacctcat catattgatt aatccctaat
    25561 gcatctccat aataatcact acaaaggtat tttgaaacta attcgatatc ttctctatgc
    25621 tgaaaatcca tattatcctc aactatcttt attaccattt tagatatagc attttctccc
    25681 ttttcaggat tacttgcatg tacagctact cctttagtag ttaactctga aataattcca
    25741 ttaataattt ttgcagtaca atgagatgga acaacatttt ctttagttcc tccccttact
    25801 attatgtaag gtttactttt gtcaatttta atttcttttt ctatccttat atgaaccctt
    25861 cctttttctc cattaaccac tggatacata gcatctggag taaatcccat acttggaatt
    25921 tcttcacctt tatctaagta atatttgata catttcatac ctgtctcctc atttgtacca
    25981 aaaattaatc ttattctttt attaattgta ggatacatat cctctatgtt tttcaataca
    26041 tgtataagca tagatacagc acccttatta tctattacac ctcttccata aataatattg
    26101 tcaattactg ctcccgaaaa tgggtctaca ctccacccct ctcctggtgg aactatatct
    26161 aaatgcattg gaatccctat taattcctct ccctctccaa tttctgcata tccaatataa
    26221 ttatcaatat ttttaacact aaaacctaaa ctagcacagt aatctaaagt tttatctaaa
    26281 gattttttag gtcctatacc aaaaggacat tgtgaaatag gttcaccttg ttgtgaatca
    26341 atttttatca attcttttag aaattctatg gttctattta aactattatc tacacttctg
    26401 cttatatcca taaatcaacc tactttctat ctttttaaag cctgtcctta aagaacaggc
    26461 ttttagcatt aaaaattatt tttttgtaaa tactaattca caaacttcat catcatgagc
    26521 acatttttga gcaaaatcta atttaacacc ttgtgcgcag tcaactcttc cataatcccc
    26581 ataacaagct aatctacaaa tttcttttct ttcttcttta gttaatccat attctttcca
    26641 agcagcatct aatggacagt gatggaattt taaaacgcca ttctcatctg tattttctac
    26701 taattccatt tcaaacactt tttgtccttt actgttatat aacatttctg ccatttttcc
    26761 tggagtatct gctacgttat atttttgacc tctcatttta ccaaatccat atattgcttc
    26821 tttacatata tcatctggat tagctcctaa tttttcagct tctttcataa gaagatagaa
    26881 ccatactgct ctttctcctg ttgctcctct aattccttct aaatattcct tttctgtata
    26941 cattttttct gacatttctt tctcctccta aaattaaatt ctatatttta atcactgtga
    27001 attagaatat tccaatgaat gaccctacca atgctaatac aactaaaatt actaataact
    27061 tagttattgt ccacttcttg tatttcattc cccaatatac gaagaataca acaccaagtg
    27121 gtaataaacc tggagcaatc atatttaatg tatcttgaac aacaattgct ttaccaccaa
    27181 tatcaaatga tagcggagtt gaaagcttaa ctgtagttgc agataatgct cccatcataa
    27241 acattcctag aattgaagca ccatcaatta tcttattgac aataccacct gatagaattt
    27301 tcttaattga atctctacct aaagaatatc ctaaatgaaa taagtaataa ccttcgcaaa
    27361 atagtaatat actaaacatt attgggaaaa atgcacctat aacacttcct tctgctccaa
    27421 atgaagcacc taaagctaag aatattgttt gaaatgtacc aaaatctaaa gtatctccaa
    27481 ttccagccaa aggtcccata agaccattct taacacctga aataacttct cctgggattt
    27541 ttccttcagt agctttctct tcttccatag ctaaaactgt accgtgaatt aatcctcccc
    27601 atatagcttg agtattaaaa aattgtaagt gtctctttaa cgcatctact aaatcttctt
    27661 ttctcttata taacttttct aaagccggag tgaaacttgc acaaactgct aatgcttgca
    27721 ttctttcaaa agagttggat aattctgctg tccaccacca tcttagatat gtttttgtta
    27781 catctttctt tgtaagtact cttgtcttat taacttcagt tggaccttgt ccatttttaa
    27841 cttctttatt ttttatttct tgcattatac ttcacctacc cttttgtctt ctttcataaa
    27901 ggtaattaaa agagctatac aaacaccaaa tattgcagct gcagtaattg atatttcaaa
    27961 atattgaacc ataaaaaatc ctataataaa cattggtaaa aatttatttt tacctatagt
    28021 aaatattgtt gttgcaaaac ctaatgcagg taatataccg cccattactg ttaatccatt
    28081 cattaaccat tgtggtataa catctaataa tgattgaact aaatctgctc caaaaaagtt
    28141 acatacaaaa actattggaa accttaaaac aaacccaaat attaaagaat aaagtgttgc
    28201 acatctccag atgccttttg tattaccttc ttcagcatac ttatctgcct tatgaaccaa
    28261 tacagcatta ccagttcttc ttaagttatt aactaatact cctataacac ctaaaggtac
    28321 cgcaatagat actgcaactt ctgcattaac accagtttga agtgctactg gtattgcaat
    28381 aagagcagct agacatttat ctgatggaat atttccccct gctgcgacca ttcctagata
    28441 aaccatttct atacttgcac caacaatcat accagttgtt acatcacccc aaagtaatcc
    28501 aaggctaaaa cctataaata aaggagactt tagtgttgag aaaagggtat accctgcaag
    28561 acctgctgca aaccaatacc ataatcctgt tacaattgcc aacatcaaaa cagattccat
    28621 ttataattcc tcctaattta tattttttat ttaaatttct ttaacgcatt ttcatatgtc
    28681 atagatgatt cctctggtat aacttgtagg actatttcaa ccccattttc attaagttca
    28741 ttaagttgtt ctacatcctc atttcctaat gaaacagctg taaacaccat tttctttcct
    28801 gcttcatttg gaatacctcc taattgaatc ttttcatatt taagtccagc tttataagcc
    28861 ttatttgcca tatcaacatt tttaaataga ataaatatat ttttatctcc taaagtgttg
    28921 ttttggaatg cttgtcccgc atcttctgct gttaaaataa ctacttctac tcctgatggt
    28981 gctgccattt tatatatgtc agccataaat tcatcttgac ctaagaagtc atcaactatt
    29041 attatttttt gagcttgagc gatttttacc cattttgtta taacttgccc atgaattaat
    29101 ctgtggtcta ctcttactaa tgttattttt gccattatat tcacctcata atttatattt
    29161 ttttaaattc tttaattaaa tccacacaaa ggttattatt ttttttgacg attctttctg
    29221 gtaatgtatg taatttatct tctttgctta attcttcatc tacgattaaa attgtctgta
    29281 agctaagtcc atttattgca tctattcctc ttcttatagc aacagctatt gcaacacttg
    29341 atgtagatcc tccatataaa tctgatatta taattttcac atcttcttta tctactactt
    29401 cttcaacctc tttcataaat acagaaacat ctttttcaga acttagtgat aaaacttcat
    29461 attgatttga caatccaaag tttttatttg tttcttctac taagactttt ccccaagttc
    29521 catggcacag tatgattacc ttatatttat tcattgctaa ataaactctc ctttatagcg
    29581 attaatattt tggtttaatt ctacttcttc ttcatcaggt accatttgaa ttgatacatt
    29641 aatattcttt ttaataaatt catctattaa aagaaactct tttttattaa tgtatacatt
    29701 ttgagatatt ttcttttttc ctattcctgt aggtattctt ccaacaataa gttttttgat
    29761 attaatccct tcttctatca ttctatttgc agtatctaaa tttttaatta atacaaaaac
    29821 gttatcatca gcttgattat tataaaaata tctccttgat tcacttacag ttaatattga
    29881 taaatcaata ttctttggtg ctgccatttt tatcaaacct ttagcaataa catcattggt
    29941 tgtttcatca tctactacta ctatcttttt tacacctaga tgcttaaccc atttatatat
    30001 aacttgacca tgtattaatc tatcatcaca tctgcaatat tttactaaag acatttttaa
    30061 tcaaactcct ttttctatta cattgagcta agaactttgt taatattgtt tataccagtt
    30121 tgtcctgctt caatacatgc ttctgctaac tcttctaaac tttttccctc tctattaatt
    30181 aaagcttcaa gtagcattgg aagatttact ccagtaagtg catgaaactt gccacctttt
    30241 ttaaggttta aacttgcaac attacttggc gaacctccta ataggtcagc taatactaaa
    30301 actccgtcac cttcatctaa ttcatttaac ttttctacaa aatttttatt taagtcttca
    30361 atattatcat tcctatttag tttaattgct gctgttcttt ctgctggtcc tgcaataagt
    30421 tcacagcttt ttataatttc ttcactaaaa ctcccatgag tcataactat tattcctatc
    30481 accaatttca cctcactttt cttgtcactt tttatataag caattgccgt gccaacttat
    30541 gtgtatcaat aacaaatggc tattttacag gatttaatca aaacttttta ttcaattttt
    30601 tttaaaaact tattatttcg atatgtatca aaagattttt tcaacctgta tcaaatctgt
    30661 atcaaaaaaa gtaaaaaaaa gatagccttt tggctatttt taaattttgt atcaactttt
    30721 gaatttatct gtatcatatt gtgattcaaa tatatctaat atatttgcat attccatatc
    30781 tgaaataatt atatcgaatg tctcttctac tagcttaaaa ctttctttta taactaaata
    30841 taattcttta tttctactaa ttctttcttc atatgattta taaactaaag actcttttat
    30901 tattactctt tcaatcatac atgaacaatg aaatataaac tttactaaaa gttcatcttg
    30961 aattactaaa gaatagtgtt cactaattct attaattact tcttttaata taaaataagc
    31021 cttttcccca tttaaaaaag tcaaagtttt actaatatta tcaattagta atctatcaaa
    31081 atatctacca ccattatctt tttcagcata tgtaacttcc atatctccat aatgtttctt
    31141 tatagattct ataatattaa aatataattt ctctaatgtc atttctggag ttagagcctt
    31201 tctagttgct tctattacaa taggtgtact taccatatct attgctttta cttcttttcc
    31261 tgtagcatca cgaataactt tagcaaatgt taaaatagac cccatatcag ttaaaattaa
    31321 aattcccttt cctttatcaa ttttattggc tatttctaca gcttgtacta aaatatcctg
    31381 aactctatct tctaatccca tattaattgc cactgcatgg ttacaatcca gtaaagtatt
    31441 ggctacattc accatgctag atgctgtaga ttcaccatgc attatgacta atattgctat
    31501 actctcctca tttaatccca catacgataa ataaagaaat gttgctaaaa aaattgcttc
    31561 atcttctgga aactctactc catacattaa tgacatgtta gataatattt cttttgctat
    31621 gttatattca tatgctcttt ccttcataat ttcatcttta ttaggatgct ttactatatt
    31681 tccaatcttt attctttcaa ttaaattctg aatatgtaaa gctatagctt ttattaattt
    31741 atctaatcct tctcttttgc tccatttatc atgcttttgt aaaatatatt ttacagtagt
    31801 tgcaattgaa tcattaacaa tgttgttata agctgtttga ttactactat tatatataaa
    31861 agtattcatt aaattatagg aatacttctg tatgtctcca tctataacac ttctaatttg
    31921 tgactcttta ataccttctt gttgaagttt attccaagtt tcttttatca ttccataaaa
    31981 atctaaatca tattttttat tttctattaa taaattttca ctttcaaaat ccttttcttc
    32041 tggtaaaaaa ataatatcat cttttccaat aaaactgaaa ttttgtacaa actcctcttt
    32101 tctattattt ccatataatg attctctaat attatttggt agaagagaga gccttacata
    32161 tacacattcc cttttatagg tcatataatc taaaaaagct cttgcacaaa ttaattgtat
    32221 atcgcttttt aattggccta tgtttccctt acacttatat aaaataaatg ccttaataac
    32281 ttcttttgaa acctttaact taacaccaat cctaacactc tcttctttaa aaaaataaca
    32341 tataagcttc attctctctt gaaagcctct ttcctctaaa gacggaatct ttatagttac
    32401 aggtattctt cttaaaaacg tttccaacat tgctgtttct gggtcttctg tagtggctgc
    32461 aattatcata actctacact cgtgtacctt attagcctct cctaaccttc tatatatccc
    32521 tttatccata agcagaaata gcatttcttg tccctctgca gataatctat gaacttcatc
    32581 taaaaacaat actccattat tagcttcatg tacaatacca tacttatctt tatcagctcc
    32641 agtaaatgct ccttttacat atccaaaaag ctgcgctaat aataattgct gattatcaga
    32701 ataatcagca cagttaaata tgataaatgg tgcattatca ctatcttctt ttatagttaa
    32761 actatactta tacatatatt ctgcaaatat agatttacca actcctgtag gtcctattat
    32821 taatgtactc aaccccttag gtggatatat tactgctgct ttagcctgtt ctatttggga
    32881 tcttaaactt ccatcacatc ctactaaatt aaaaatattg ttctcttttt ttatactctc
    32941 taaaatatac tcatctttga tagaatcatt ttcactatat tcgtttatag agttaatatt
    33001 atcaaatatc cctttattta gtttctttcc taaaatactc tcaatacttt ctttatataa
    33061 gtactgaaca ggcttaccct ttatctttat aatgatactt tcttttatta attgatttaa
    33121 ttccttacta gcattacttc tatttatacc tacttcagta gctatatatt ctgcatcaaa
    33181 tccaaatcta atgccattat taaagttcaa gtgttcttct gttaaccttt tcaagcaaat
    33241 taaaactttt tctttccttg atagcttctt ttgtgtcata gttaacctcc cctataagca
    33301 gtaatactgt taaacattta ttattaagct taattctaca atattttaat atttttgtct
    33361 aattatgtaa ctattgattt tctttcctat tcgttttata atataaatta cgttaccctc
    33421 atcttttata gaggaggaat ttatatgaat tttttatcag atttacacac ccactctata
    33481 gtcagtggtc atggctatag cactttacta gaaaatatta attattgtaa agaaaatgga
    33541 attaagatac taggtacatc tgaacatggt cctaagatgc ctggttctcc ccacagatgg
    33601 tactttcaca acattcaaaa tatcccaaga atcataaata acattataat tttaagaggc
    33661 tgtgaagcaa atatactaga tattaaagga aacatagacc tagagccttt tgtaattcct
    33721 cggttagatt atcttatctt gtcatttcac gaagctgtgt tttctccaaa tacattagag
    33781 aataatacta aagctttaat aaatgcaata aataagcatg ataatattga aatattagga
    33841 caccttggaa acccaaacta cccaatcgac tatgaattaa tcattaaatt agctatagaa
    33901 aagaatatct taatagaaat aaataattgt tctattaaag gcgtgtcaag aaatggtagc
    33961 tcggataact gtaagtatat tgctacactt tgcaaaaaat atggtgctaa aatcatacta
    34021 acttcagatg ctcacatttg ttttgacata ggtaactatg agtattctga aaatatctta
    34081 aaggaaatta attttccaga tgaattaata atgaattatc ctaaaaaact tattaatcat
    34141 tttcatcaaa aaggtaaatt actagatgtt gattatacaa atatctaagt aataatacat
    34201 gcttacttta actcccataa aaattttaat actatttata ttatatgaaa taaagaaatt
    34261 ataataaaga agctatttaa taaagcttaa ttacttttat gaacttgatg gattgatatg
    34321 gtctatgata gcaatagaaa ttataacatt tttaataagc ttagttttat ataccttata
    34381 gtataaagct aaaaaaaaga atcatctaaa ataaagtaaa tcttatataa ggtttacttt
    34441 atttttattt gtaataatca tcaatacaaa ttattttatt atatgtaatt atttatgtct
    34501 taaccaattt tatcgaaaat aacagtcttc tcttttaaag agatttttcc tacttgatat
    34561 ccatattcat ttaattcctt tttatatttc aaaaacgaat ggtctatctc tatcccagca
    34621 atatctttta tttcttcaaa agttaattta aacgattgac tattgttttt cttaacatat
    34681 tcccataaag aactatattt actcatctta tatcactcct tttaaaatca tatcttatct
    34741 attaatttat attctatcat tattaatgta taaagtcatg ctacttatta tttatatata
    34801 aaaatatcca aataataagt agcaattttt tatagtcata atattttttt cagattaaat
    34861 aaaaatcaat tat
//
LOCUS       DAMJVK010000037        34806 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.037, whole genome shotgun sequence.
ACCESSION   DAMJVK010000037 DAMJVK010000000
VERSION     DAMJVK010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34806
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.037"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(122..778)
                     /locus_tag="PC414_001860"
     CDS             complement(122..778)
                     /locus_tag="PC414_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2464257.1"
                     /translation="MELQDTIFKRQSVRKFKNQDVSDEDILKMIKAAGAAPSGKNIQN
                     WHFVVIKRRDLMEKIADVITKKQQEILVEMDKVSVDKANRFRKFVKNFTLFYLKAPVL
                     VLVFTKVYNPSGYYELELIDAPKETIDKLFIRNPGMQSLGAAIENFTLSAIELGYGSC
                     WLTSQNYAADEIEAVLEAETGFEKGEYFLGAMLALGVPEDNLKSPSKKPVEEICTFIK
                     "
     gene            complement(833..1462)
                     /locus_tag="PC414_001861"
     CDS             complement(833..1462)
                     /locus_tag="PC414_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDF2464258.1"
                     /translation="MVDSIIFDLDGTLWDSTEEVCKVWQDVLSEHKEIELSVTKDLFR
                     SLMGLSFEEIANRLFPNLSEEERMNLLNECSLKECDYLSENGAKLFDDIEDTLKLLSK
                     KYKLFIVSNCQAGYIESFLKAHKLEQYFIDFECPGNTGLHKGENNKLIIERNKLINPI
                     YVGDTQGDANSAKFVGIPFIYAKYGFGNVDVYDYFIDSFKDLLEHDILK"
     gene            complement(1593..2234)
                     /locus_tag="PC414_001862"
     CDS             complement(1593..2234)
                     /locus_tag="PC414_001862"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HDF2464259.1"
                     /translation="MKICIVLNGEIEDYEVTRDIIIKECYDCIICADGGANHTYKMEI
                     MPDYILGDLDSVEEEKINFYKNKGVKFEKFPSKKDETDTELCLFLAKTLKANHIDFFG
                     ALGGRIDHTLANIKLLYYLKEDGIYSRILSDKEEMYIVENEEISLYGNPGDTISVIAI
                     NGDAKGVTLTGLEYPLDNYYMKYSVPIGISNVMLSNSCKIKVEQGCVLVVRNL"
     gene            complement(2243..2914)
                     /locus_tag="PC414_001863"
     CDS             complement(2243..2914)
                     /locus_tag="PC414_001863"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDF2464260.1"
                     /translation="MIKLAPSILSADFAKLLEDVRKVESAGCEYLHIDVMDGHFVPNI
                     TLGPLVVKSLKKENINMVFDAHLMIENPDQYIEEFVKAGCDIITVHQEACVHLHRTIQ
                     NIKSHGIKAGVVLNPATPVDTIKHVLPDLDMVLLMSVNPGFGGQSFIPCVLDKIKELK
                     AIIDSQGLNIDIEVDGGISPKNVAEVVQAGANVIVAGSAIFGSDDIQETVNLFRKNAS
                     LEELV"
     gene            complement(2919..3803)
                     /gene="rsgA"
                     /locus_tag="PC414_001864"
     CDS             complement(2919..3803)
                     /gene="rsgA"
                     /locus_tag="PC414_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HDF2464261.1"
                     /translation="MLEGKIIKGISGFYYVDTYNGIYECKARGIFRKQKITPLVGDRV
                     KISIVDENEKKGILEEIDSRDTELIRPPIANVDKALIVFAIKNPKPNLSLLDRFIVLA
                     EKENLETVIILTKADLDDNDTLETVKNIYELSGYKVIPVSNITKLNIDKVKEELKENV
                     VVFAGPSGVGKSSLLNEIDENFKLQTGVVSDKIKRGKHTTRHAELLKLEFGGMVADTP
                     GFSSLALEDIEEVELKDYFIEFDKFNDCKFGSKCIHENEPNCAIKEAVTNGEISKERY
                     DSYIQLLHEIRQNNSRRY"
     gene            complement(3901..4638)
                     /locus_tag="PC414_001865"
     CDS             complement(3901..4638)
                     /locus_tag="PC414_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDF2464262.1"
                     /translation="MKKKTVLITGGARGIGKAMSKAFAKEGYNVLVNFNKSENEAKEL
                     YTILNEKNFSVKLFKADISNREDVEDMVDYCIKEFGGLDVLVNNAGVSQDKLFTDITD
                     EDWDNMMNINLKGSFYCSQVALKYMISEKKGNIINISSIWGISGASCEVHYSITKAGI
                     IGMTKALAKEVGPSNIRVNSIAPGVINTDMLSGYNEEDIDALVEETPLMRLGTPEDIA
                     NCAIFLASDKSNFITGQVISPNGGFVI"
     gene            complement(4746..6761)
                     /gene="pknB"
                     /locus_tag="PC414_001866"
     CDS             complement(4746..6761)
                     /gene="pknB"
                     /locus_tag="PC414_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HDF2464263.1"
                     /translation="MGDTILGNRYEIIRKIGDGGMAFVYEAKDRLLNRTVALKVLRPE
                     FVDDDEFLTKFKREAEAVASLSHPNIVNVYDVGEDGKVHYIVMEFVDGKNLKEIIQDE
                     GILDEYTALDITKQIAMALSAAHKKGIIHRDIKPHNILISNEGRVVKVADFGIAKAVS
                     NSTMTNIGSIIGSVHYFSPEQAKGKFVTNNADLYSLGIVLYEMLIGKVPFRGDSPISI
                     ALQHINDDIDFTSEEKVRIPQSVRTTIKKLTEKSSADRYQTAEELIEDIEYIEKNIDL
                     DFIKEYDDFATKKIDEKEINKVVNPTLAKPAPEKVVKPVEVADLDDDEDYYDDFYEED
                     DDEDEEEEEIMRAKKNQRPKSTPSKRTKKKKKKQESPKSRRRLKVIAAVLILILCAQV
                     FLAYKFLFAGGFGNKSLTVPNLVNMTLEEAQSAVEKEGLYLSVKSEEYNSEVDENCII
                     SQTPEGGSTNVKKGDTINVVVSKGSSQASVPNVVGLTLSNAKQLIEENNLKVGTVKYE
                     YSSIYKEGTVLSQSPGAGSSRAQEGDEVNLYVSKGSEKSNTQTPTVPDKKPTTPENNT
                     PTEPGSNSGNSGGNSGGSNSGNSGGNSGNNGSNSGGSNSGNSGGNSGGSNSGDNGGSS
                     GGSNSGDNGGSSGGSNSGDNGDSSGGSNSGDNGGTSSGTGANIGKTE"
     gene            complement(6778..7530)
                     /locus_tag="PC414_001867"
     CDS             complement(6778..7530)
                     /locus_tag="PC414_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HDF2464264.1"
                     /translation="MVYSCASHIGKIRKNNEDYCEGEVIDTEHGPIGIFAIADGMGGH
                     KKGEVASKLAVENIIDFLKENLLQHDNVKIDYIDDILKQAYNNVNSIVHKKSMEDIEF
                     EGMGTTLVTAIVYNNVLYVANVGDSRCYLLTDEKFDKITIDHSVVEELMMAHVITEEE
                     ARRHPQRNRITRAIGTDDMVVVDIFKKEIKKSDIILLATDGLTGFIDDEDIRDLILDY
                     EYERTSNISEELISMANDVSGKDNVSVIVIKV"
     gene            complement(7550..8581)
                     /gene="rlmN"
                     /locus_tag="PC414_001868"
     CDS             complement(7550..8581)
                     /gene="rlmN"
                     /locus_tag="PC414_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HDF2464265.1"
                     /translation="MEKKKIVLKNFTEDELKEFMKTIDEKPFRGSQIFSWIYKGAKTF
                     DDMNNIPKSLRNKLEEVSCIGHIDIELKLESKVDNTKKYLFLLDDGNIIETVMMDYDS
                     RVTVCVSNQVGCRMGCNFCASTMDGLIRNLEPWEILDQVIKIQEDTGKRVSNLVLMGS
                     GEPLDNFENTKQFLKIINEKNGLNIGYRHITLSTCGIVPKMYELADLEIAINLALSLH
                     SPYDEERRKIMPVANAYSIEEILNACRYYIKKTNRRVTFEYSLIKGVNDSEKEAKALA
                     KLLKGMLCHVNLIPINKVEEREYEKPDKAFIYKFRDSLEKNNIPATVRMSMGSDISGA
                     CGQLRRKYK"
     gene            complement(8589..9914)
                     /gene="rsmB"
                     /locus_tag="PC414_001869"
     CDS             complement(8589..9914)
                     /gene="rsmB"
                     /locus_tag="PC414_001869"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDF2464266.1"
                     /translation="MDAREVGFKVLCDIEKNDNYSNIAINKHFKNLEISDMDRGLATE
                     LIYGVIENKYYLDYIINKLSKIKVKKMSTYVKIFLRMGTYQILFLDSISDYAAVNETV
                     KLSKKYDKKSSGFINAILRNEIRAKETIMDITEEDSVKYLSIKYSYNSWIIKNWIDKF
                     GQEFAEDLLEANNEKPSIYIRTNTLKISREELIEKLSEMGIVCLKVPMVEEAIKVEKM
                     KNIEKNELFKAGLFTIQDISSMIVGKVINPKEGSFVLDVCSAPGGKSTHLATLMNNTG
                     QVVARDIFEHKLKLINSTVNRLGLKNVFVERFDASEIDENSISKFDYVLADVPCSGFG
                     IIRRKPEIKYKKEEELKDITSIQKKILENASKYVKVGGTLVYSTCTIQDVENINIVTS
                     FVEENHNFEFVPIDTVNVDLDNQDKGYLKIYPNIHGIDGFFIAKLKRIR"
     gene            complement(9976..10677)
                     /locus_tag="PC414_001870"
     CDS             complement(9976..10677)
                     /locus_tag="PC414_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HDF2464267.1"
                     /translation="MYPVYGGFWGFDPTMVVLIPAILLTIYAQFKVSSTTNKYLRVNT
                     RRGYTGEQTARRVLDSNGLYDVKIEMVRGHLSDHYDPRRKAVRLSEDVYYGTSITSVA
                     VAAHECGHAIQHAKGYAPLQIRSSLVPVVNFASSISWFLIFLGFIMAGPFLKIGILLF
                     SASVLFQIITLPVEFNASSRAIVQLGNLGIIDESESRQSRRVLSAAALTYVAAALVSI
                     LQLLRLLLIAQRRND"
     gene            complement(10746..11498)
                     /locus_tag="PC414_001871"
     CDS             complement(10746..11498)
                     /locus_tag="PC414_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HDF2464268.1"
                     /translation="MTDIKKYLTSVGILILILGILIGVVNFFIISLTQIFSLMINIII
                     ISLILIILCSTAVTYRVIKGKYVNSNIMKINFNIVSGLFPIISFIASSFGMSKSDIRR
                     IYIKLNNEYIYSNKYNFNPEDIIILIPHCIQENSCKLKVTNDIDNCKECGRCNIGELI
                     KLKEKTNVKIFVATGGTLARKIIMDNKPKAVVAVACERDLTSGIQDIKKIPVLGVFNK
                     RPNGPCVNTNVDIMDIEKAIGFLTGKNILVCN"
     gene            complement(11508..12437)
                     /locus_tag="PC414_001872"
     CDS             complement(11508..12437)
                     /locus_tag="PC414_001872"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDF2464269.1"
                     /translation="MKIVFMGTPDIAVPCLQKIIDEKYEILGVVTQPDKPKGRGKKLG
                     MSPVKELAIENNIPVYQPVKARDKEFIDKIKSLNPDVIVVVAFGQILPKEILEIPKLG
                     CINVHVSLLPKYRGAAPINWVIINGEEKTGVTTMYMDEGLDTGDMILKTEVNLDENIT
                     AGELHDKMMNIGAETLKETLRLIEEGNAPREVQNHEEFSYAPIMNKSLGNIDFSKSAR
                     EIHNLVRGVNPWPSAYTTYNDVIMKVWKTKVLDEKSTKDVGTIIDVSKDGIKVSTIDN
                     VLLIEEIQMPNKKRMLVGEYIKGNTIETGLVLG"
     gene            complement(12452..12892)
                     /gene="def"
                     /locus_tag="PC414_001873"
     CDS             complement(12452..12892)
                     /gene="def"
                     /locus_tag="PC414_001873"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDF2464270.1"
                     /translation="MALRQIVQIGEPVLRKKSKKVEKIDEKIIQLLDDMAETMYDADG
                     VGLAAPQVGILKRVVVIDIGEELIELINPEIIETSGEQIDEEGCLSVVGEAGNVRRPN
                     YVKVRALNRNGETIELEGEELLARAFCHEIDHLDGILFVDKIEK"
     gene            complement(12910..15435)
                     /gene="priA"
                     /locus_tag="PC414_001874"
     CDS             complement(12910..15435)
                     /gene="priA"
                     /locus_tag="PC414_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDF2464271.1"
                     /translation="MNNILNLSRGFIMKKYAKVIVRSNTIYTDNLFTYQIPVFLSDVI
                     KIGHRILVPFGKGNKPTEAFVFQFTDSLDEKIKIKEIIDILDENPIFRKEDLELVYWM
                     KNRYLCTYIECINLIYPKGYKLNNYKVVLLGESLSGLNDIELKEKISTLSDKNREIVE
                     KVIDSKGKIKVDKLKYIPNLNSSLYTMNKNGIIKLCWEYKNHKNEKKVCYISLSLESD
                     KIDDYIEQNKINVGSKQKEILSFLKNNENVEINDLLDLLNASKQSINSLSKKKLITLE
                     FKDYYREPKSIYKSVLKSIKLNNEQQEAVDEIKSNMFVDDKKPYLIHGVTGSGKTEVY
                     MEIIEFALNQGLDSIFLVPEIALTPQTIDRLKSRFGDLVGVFHSKLSEGEKHDVYKAV
                     KAGKVRVLIGARSALFAPFNSLGLIIIDECHESSYKSEKNPKFNAIEVARFMALKNNI
                     TLILGSATPSIEEYYRAKSGEYKLINIKSRANDKPLPNIEVVDMKDELDKGNRSIFSL
                     KLQKEIRYAIEENNQVILFLNRRGYANFVSCRKCGYVFQCENCDISLTYHKKSNTGRC
                     HYCGYEKEIPKECPECKSTYVKPFGVGTQKIEEELKYIFPDIKTLRMDKDTTSKKGAL
                     DEILNKFKDKEADVLIGTQMLSKGLDFENVTLVGILSADMILNFPDFKSFETTFQLIT
                     QVSGRAGRADKEGKVVLQTYDTEHYAIKHAIEYDYEGFYEDEIKIRKAFGYSPFNNML
                     SVVVSGEDERLVIKNIKNMHASLIYLLEKRGINDLGFILGPNPCSISKINQNYRWQIL
                     FKDENIEINLLKGIIKYICITKRDLIFDKNINVSIDINPNSVL"
     gene            complement(15674..16873)
                     /gene="coaBC"
                     /locus_tag="PC414_001875"
     CDS             complement(15674..16873)
                     /gene="coaBC"
                     /locus_tag="PC414_001875"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HDF2464272.1"
                     /translation="MLKDKTVVIGISGGIAAYKACDVVSKLKKLNANIHVIMTKSATE
                     FVKPLTFQSLSQNYVVEDMFEEPKTWEVEHISLAKKADLFLIVPASANVIGKIANGIA
                     DDMLTTTVMATKAQVLIAPAMNTNMYENPIVQKNIQTLKSFGYKFIEPESGRLACGDI
                     GTGKLANPDIIVEEVLTNLTKEQDLKGQSMIVTAGPTVESIDPVRYLTNRSSGKMGYS
                     IAREAIARGADVTLISGPTNLSIPQNLKKFVQIESAQDLYEAVVSNLSENKIVIQSAA
                     VADYKPKTYSDKKIKKKNDDLSIELSRNYDIAYEIGKIKEDKILIGFAAETNDLIEHA
                     KGKIKKKNLDFIVANDLTKEGAGFRADTNIVKIIDVEGNITEYPKMKKEEVANVILDK
                     VKALLEK"
     gene            complement(16878..17144)
                     /locus_tag="PC414_001876"
     CDS             complement(16878..17144)
                     /locus_tag="PC414_001876"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDF2464273.1"
                     /translation="MLKPSINEVLEKIDNRYYLVGTVSKRARKLIDGEEPYVSNKTKE
                     KPVCVATKEVASGKITYRLLTEEEIEIEEARHHAEQHQQISEEE"
     gene            complement(17147..17764)
                     /gene="gmk"
                     /locus_tag="PC414_001877"
     CDS             complement(17147..17764)
                     /gene="gmk"
                     /locus_tag="PC414_001877"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDF2464274.1"
                     /translation="MFVKKGLLLVVSGPSGAGKGTICKELLKENDTIKLSVSATTRKP
                     RTGEVDGVNYFFISKEKFEEMIEKGEFLEYAQIYDNFYGTPKAAIMECLEKGQDVLLE
                     IEMQGAKQIKEVCPEGVFIFVLPPSLKELKNRIVGRGTETEAEIEKRFSCAYEEIKMI
                     KDYDYFIFNEDVKTSAKEIEGIISSEKNKVSRYKNIIIEKFKEEL"
     gene            complement(17794..18675)
                     /locus_tag="PC414_001878"
     CDS             complement(17794..18675)
                     /locus_tag="PC414_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HDF2464275.1"
                     /translation="MAISMTGFGRGEYKDDNYYFLVECKTINHKYSDINIRLPRKISF
                     LEDKVRNLVKNYVKRGRVDLYIKFDLLGKEDVNLNFDEGLASQYIDILKEIKNKFDII
                     DDISVMNVAKFPDIVKIEEKEEDEDLLWSMLNQAVEDALIKLREMRSEEGKKLAEDIA
                     MRCDLLKNHIEEIEKYSSSVVEDYREKLNLRISELLDDPSIIDENRLAQEVAIYADKS
                     SITEEIVRFKSHIGQLKNTIFKDDSIGRKIDFLIQEMNRETNTIGSKSSDINITNLVV
                     EVKSELEKIREQIQNIE"
     gene            complement(18679..19509)
                     /locus_tag="PC414_001879"
     CDS             complement(18679..19509)
                     /locus_tag="PC414_001879"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HDF2464276.1"
                     /translation="MKFWKLHGIGNDFIAIDGRFDQINPSDYTALAKKVCHRRFSVGA
                     DGLLVVKDSKVADVEMVYYNSDGSRAAMCGNGLRCFCKFVYDNCIVKKESFTVDTLDG
                     IKDIKVNLTNREINSIRVNMGKGSFIAKDVPVLTNKERFVQESIKVLDRELKVTSMLM
                     GVPHTVVIVDELDVDDVCKYGKEIENNKIFPEKTNVNFVKVEDKYNIHVYTWERGCGY
                     TLGCGTGMTASAIVCNLLNMVESSVNVTSQGGTVKIDVGDFSYMTGPAVKICEGILEV
                     "
     gene            complement(19796..20830)
                     /gene="ltaE"
                     /locus_tag="PC414_001880"
     CDS             complement(19796..20830)
                     /gene="ltaE"
                     /locus_tag="PC414_001880"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HDF2464277.1"
                     /translation="MRFIDLRSDTVTMPTDEMREAMAKAPVGDSIFRDDPTVNKLEEL
                     AAAKVGKEDAIFLPSGTFGNQLALFTHCLRGQEIIIGKGYHIVTHEVGAPAVIAGVQL
                     RTIDEDESGALNPELVEKAIRRDDIHEPQTGLICVENAYSGGTVVSLDNLREIKKIAE
                     KHNLPVHLDGARLFNAALTLGVEAKEIAKCCDSVMFCVSKGLAAPMGSILAGNKEFIA
                     KARRKQKVMGGGMRQVGIVAAAGIIAIEKMTLRLNEDHENAKYLACELNKIDGIEVNN
                     VNPDISMVFFKMSEDIIKEDVLIKEFFERNIKINKMENGEYRFVTHVDITKEDLDYVL
                     KTLKELIGVC"
     gene            21126..22901
                     /locus_tag="PC414_001881"
     CDS             21126..22901
                     /locus_tag="PC414_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HDF2464278.1"
                     /translation="MALDGLVIHSIVDELSSKLTGGKIDKIHQPEDDEVIFNIRNNKE
                     NFRLVLSASASNPRVYLTSNYQKENPLKAPMFCMLLRKYIQGGNIVEISQIGFERIIK
                     ISVESLDELKEKTVKNIMIEIMGRHSNIIITHGEENKIIDSIKRVPFSISRVRQVLPG
                     HDYSLPPEQNKLNPLDDISKDLFIKNLEELEGPIFKSIYSKFLGISPIIAKEICYRAG
                     VNQNAIIKDISDEQFDSLHKVFCNLFNDINSNKYSPCIIIDKKVDKVVDFSCINLTLF
                     SDLSYINKDSMSRILEDFYRTKDIKDRINQRSSDLKKSISVKLDRLYNKLKKQEEELS
                     ESENADIYKIKGELITSYIYMVEKGMESIEVANFYDENCNDVTIELNKNLTPSENAQK
                     YFKKYNKMKHAKVEISHQISLNKEEIDYLENIILSIENCENLAELQDIKEELAKVGYI
                     KTQKKNSKKDTIPSTKPHEFVSSDGFKILVGKNNKQNDYLTLRLADNDDLWMHTKNIP
                     GSHVIIKCAGKEVPDNTVFEGAMLAAFFSKSKLSSQVPVDYTKRKNVKKPSGSKPGMV
                     IYETNSTIYVTPEEETVAKLKVKSE"
     gene            complement(23116..24768)
                     /gene="abc-f"
                     /locus_tag="PC414_001882"
     CDS             complement(23116..24768)
                     /gene="abc-f"
                     /locus_tag="PC414_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein"
                     /protein_id="HDF2464279.1"
                     /translation="MLLVKVENLKKYYADKLILDIDKLEILENDKIGLVGSNGQGKTT
                     LLKAILGEIEIDEGYTYLTESYSYISQSENNIETCSHSKEKSLLNAPDKFEEHLSGGE
                     KVKLKIADALSNKKNIIIADEPTSNLDKKSIGVLEDMFKRHEGALLLISHDRRFLDEL
                     CTTILELEDGKLKAYKGNYTDYLMQKDEEVKRADFEYQEYVKEKKRLEKALLYKKALS
                     DGIRKTPKRMGNSEARLHKMGGQTNKKKLDSNVKAIKSRIDKLEVKNKPKVSKEMNIK
                     IQDGMEIISKNLVEVKDMTLKLENKLLLDNVSFKIKRGKKIALLGDNGCGKSTLIKEI
                     LADKNDNIKINNKVKVGYFDQNQSLLDEEKSVLYNTKVNSSFDESFIRINLSLFGFKG
                     DDVYKKVKVLSGGEKVKIALCKIILEDNNFLVFDEPTNYLDIKSMEALEKALINTDKT
                     MLIVSHDRVFVSHICNYIIEIKDAKIREFDCNYDEYTISRNKKTPSRENQIKKENLLV
                     LENRLTTVISMLSIEKDNLKKELYESEYNELLKQINKLKNSF"
     gene            complement(25067..26278)
                     /locus_tag="PC414_001883"
     CDS             complement(25067..26278)
                     /locus_tag="PC414_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HDF2464280.1"
                     /translation="MKKTIMSLQHLLAMFGATVLVPILTGFNPSVAIFCAGVGTLIFH
                     FCTEGKVPAFLGSSFAFIPVILAAKEAYGGDLAYAQGGIIVAGLIYIIMSIIVKVVGV
                     NKIKLYFPAQVTGAMIVVIGLNLLPTAFSMASANFVIAFITLAIAILTNKFGRGFIKQ
                     LGILIAVFSGYIICLILRLVDITTITEASLFAIPNFTVPKFSLGAIVIISPVVLAVFM
                     EHIGDMTTNGAVVGKNFIENPGLNRTLLGDGFATIVAGCLGGPANTTYGENTAVLAIT
                     KNYDPSILRRTAIFAILLACVGKFGGFLQSIPGSVMGGISIMLFSMITYVGLKTIRDS
                     SCVESKINILIIAVILLIGLGTTYLSNKGISVGIPITSTVKITGLSLAAIVGIVLNRI
                     LNNQDFKVEEE"
     gene            complement(26562..27095)
                     /gene="pyrR"
                     /locus_tag="PC414_001884"
     CDS             complement(26562..27095)
                     /gene="pyrR"
                     /locus_tag="PC414_001884"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001156491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HDF2464281.1"
                     /translation="MVEKAQLMDEKAIARAITRISHEIIERNKGVENLVLVGIKTRGV
                     PIANRISKKIEQIEGTKVDTGDIDITLYRDDLEKIHVEPVVKGTYLDFNVNDKTVVLV
                     DDVLYTGRTVRASLDAIIDIGRPKSIQLAVLVDRGHRELPIRADYVGKNVPTSRHEII
                     SVSLLEIDGEDSVTIKE"
     gene            complement(27455..28402)
                     /locus_tag="PC414_001885"
     CDS             complement(27455..28402)
                     /locus_tag="PC414_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDF2464282.1"
                     /translation="MFYFLKVKVGDIMDEVKEFLVLEEEEEVRLDIYLAEQLGDMSRS
                     YIQKIIKDGKVKVNNKVEKARYLVKEEDNIVIEIPEPKVLEVLPQDIPIDIVYEDDDV
                     LIINKPQDMVVHPAPGNYENTLVNGILYHCKDKLSSINGVIRPGIVHRIDKDTSGLLM
                     IAKNNYAHNFLAEQLKEHTITREYEFICYGVVKEDKITVDKPIGRNPKDRLKMAVVKD
                     GKNAITHFEVVERFDKFTHMRARLETGRTHQIRVHALSINHPLLGDYVYGPKETKFKL
                     KGQTLHAKKLGFIHPTTKEYIEFNSELPEYFKEVIKKIK"
     gene            complement(28371..28817)
                     /gene="lspA"
                     /locus_tag="PC414_001886"
     CDS             complement(28371..28817)
                     /gene="lspA"
                     /locus_tag="PC414_001886"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDF2464283.1"
                     /translation="MLYILIIILLIGLDQLSKIWVLNNLVDVSTIPIINNVFHLTYVE
                     NRGAAFGLLQNNQWIFIIVALLATVFGLYYLNTRKVHIFGRLGIILIISGALGNLVDR
                     VRLGFVVDYFDFRVIWEYVFNVADVFVVVGTVFLCIYVLFFESKSR"
     gene            complement(29127..29906)
                     /locus_tag="PC414_001887"
     CDS             complement(29127..29906)
                     /locus_tag="PC414_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2464284.1"
                     /translation="MKKIICGICICLLSIVGVYTYNHNTHNQDSSKEAYSLNDNIKDD
                     YEDVIIKKGNEDEKILALTFDDGPDEDFTPQVLDILKKNDVKATFFVVGEKVEYNKEL
                     LKRQYDEGHEIGNHTFTHINVAKNSYSSVEKEITDTQNIIKSVIGVEPKIFRPPYRAM
                     SKSVCDIIVSKGMNIILWSNLDPRDWSNPGVDAIINTILTKVQNGNIILLHDYNTKRN
                     DKSQTIQALEVVIPKLKERGYKFVTVSELIQHLDKNKQVQK"
     gene            complement(29961..30443)
                     /locus_tag="PC414_001888"
     CDS             complement(29961..30443)
                     /locus_tag="PC414_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HDF2464285.1"
                     /translation="MDTKKYKDKLLKERENLTNLVEDMKDNTLFGDTTKHTSEKYSSG
                     ELSSYDNHIGDMGTDLYMQNMQNSLINHEEGRLYQIDLALSKIENGTYGICDLCHNQI
                     DLERLDILPDTSLCNDCAKKENNLLGDAMQNPDVEDSNFYSEDLTNLTDLNKNGILRD
                     "
     gene            complement(30709..32130)
                     /locus_tag="PC414_001889"
     CDS             complement(30709..32130)
                     /locus_tag="PC414_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HDF2464286.1"
                     /translation="MVSFLGSIVVLIVGYFVYGTFVEKVFGTNDKNQTPAIACRDGVD
                     YVPMKWKRIFLIQFLNIAGLGPIFGAIQGALFGPSAFLWIVFGTIFAGGVHDFASGYL
                     SMKNKGTSASELVGLYLGNGAKIAMRIFSVILLVLVGVVFVTGPAGLLKTLTGVNAQI
                     WVGVIILYYIMATVLPIDKLIGKIYPLFGAALLIMAVGVAGGLIIKGYNIPNINLQNM
                     NPNGTPLFPYLFITIACGAISGFHATQSPLMARCVEKESEARPVFYGSMVAEGIIALV
                     WAAAAMSYFHGIPQLNVIFNDGGAATVVNTVSVGLMGPIGGALAILGVVACPITSGDT
                     AFRSARLTIADAMNYNQDAVKNRFLIALPLFAVGVALTFIDFNIIWRYFSWANQTLAM
                     IMLWTGSAYLVKANKNHYITTLPALFMTVVTFSYIMQAKEGFRLPVNISNGIGVIVAI
                     ILGLLFFKKAKHIKEQNNHKLAS"
     gene            complement(32276..33046)
                     /locus_tag="PC414_001890"
                     /pseudo
     CDS             complement(32276..33046)
                     /locus_tag="PC414_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295458.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(33051..34253)
                     /locus_tag="PC414_001891"
     CDS             complement(33051..34253)
                     /locus_tag="PC414_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDF2464287.1"
                     /translation="MKTSILIALIEKTSLIVVLFLLITKLKIFKQIFQKEEYSFNDLV
                     CIALVFTFLAIFGTYSGINYMGSIVNTRIISIVSGGILFGPMVGITAGVFSGIHRYFM
                     DIGGITSVPCLLSSILAGVLSGFLYKRIPKQHRVMYGILVGMISESFTILLIYLISYP
                     HSLAIQIIGGIYLPLIVGQIGIGFVISIVEGIEKDKKDIEARNKAEIKALQRQINPHF
                     LFNSLNTIASFIRFSPDKARELIINLSTYLRYNLEYSDNLIDINKEIEQVKSFVEIEK
                     ARFGELLTVSYDIEDVNIKIPSLIIQPLVENAIIHGILESGRAGVVKISIKKLPYSLE
                     NTVRISIEDNGIGISEEIINNVYQDNMPENKIGLYNVHLRLKLMYGRGLNIRRIDNGT
                     LIVFYVKE"
BASE COUNT        11088 a         6389 c         3487 g        13842 t
ORIGIN      
        1 ttaaatcaat gatttgaaat ataaaattaa tatcaataaa acctactttt taatttttta
       61 aatatttatt tttaatttat tttgttttta atttacttcg attttaaaat gtccttgact
      121 tttattttat aaatgtacat atttcttcta ctggtttttt agatggactc ttcaaattgt
      181 cctctggcac tccaagagct aacattgcac ctaaaaaata ctctcccttt tcaaaaccag
      241 tttctgcttc taaaacagct tctatctcat ctgctgcata gttttgactt gtcaaccaac
      301 aagaaccata tccaagttca attgctgata gtgtaaagtt ttcaattgca gcacctagac
      361 tctgcatacc tggatttctt ataaataatt tatctatagt ctccttaggt gcatctataa
      421 gctctaattc ataatatcct gatggattat atacctttgt aaaaactaat actaaaactg
      481 gagcttttaa gtaaaatagt gtaaaatttt taacaaattt tctaaatcta ttagccttat
      541 ccactgatac tttatccatt tctacaagta tttcttgttg ctttttagtt atcacatctg
      601 ctatcttttc cattaaatca cggcgtttta taacaacaaa atgccagttt tgaatattct
      661 tgcctgatgg tgctgcacct gctgctttta tcatttttaa aatatcttca tctgatacat
      721 cttgattttt aaactttctt acactttgtc ttttaaaaat agtatcttgt aattccattt
      781 ttaaatcccc ccataacttt tttattattt taatataatt tttaaactca tcttatttta
      841 atatatcatg ttctaacaaa tctttaaatg aatctataaa ataatcatat acatctacat
      901 tcccaaaccc atatttggca tatataaaag gtatacctac aaacttagca ctatttgcat
      961 caccttgcgt atcaccaaca tagattggat ttattaattt atttctttca attataagtt
     1021 tattattttc tcctttatgc aaaccagtat taccaggaca ttcaaaatct ataaaatact
     1081 gttctagttt atgtgctttt aaaaaagatt ctatatatcc agcttgacaa ttacttacta
     1141 taaataattt atatttttta gatagtaatt taagagtatc ttctatatca tcaaataatt
     1201 tagctccatt ttcagataaa tagtcacatt ctttcaaaga acattcattc aataaattca
     1261 ttctttcttc ttcacttaaa tttggaaata aacgatttgc tatttcctca aaagaaagcc
     1321 ccataagact tctaaataaa tcttttgtga cacttaattc tatttcctta tgctcactta
     1381 aaacatcctg ccagactttg catacttctt cagttgaatc ccacaatgtt ccatctaaat
     1441 caaaaataat actatctacc acaaaaaatc ccccttatgt caaagtttat tatatattta
     1501 taattatata ctaaaaagca gatacaatca taaatatatt ttacttgatt gtatctgctc
     1561 tacaatataa aaccaattgt caaaatcaaa ttttataaat ttctaactac aagtacacag
     1621 ccttgctcta ctttaatctt acaagaatta ctcaacataa catttgatat tccaataggt
     1681 acagagtatt tcatataata attatcgaga gggtattcta agccagtaag tgttacaccc
     1741 ttagcatcac catttatagc tattacagaa atagtgtccc ctgggtttcc atacaatgaa
     1801 atttcctcat tttcaactat atacatctct tctttatcag aaagtatcct agaatatata
     1861 ccatcttctt ttaaataata taatagcttt atatttgcaa gagtgtggtc aatccttccc
     1921 cctaaagcac caaaaaaatc aatatgattt gctttaagtg tcttagctaa aaaaagacaa
     1981 agctctgtat cagtctcatc ttttttagat ggaaattttt caaatttaac acctttattt
     2041 ttatagaagt ttatcttttc ttcttctact gaatctaaat ctcctaatat ataatctggc
     2101 attatttcca ttttataagt gtgatttgcc cctccatctg cacatattat gcagtcataa
     2161 cattccttaa ttataatatc tcttgtaact tcgtaatctt caatttcgcc atttaaaaca
     2221 atacagattt tcattattaa atttaaacta attcttcaag agaagcattc tttctaaata
     2281 aattaactgt ttcttgaatg tcatcacttc caaatattgc agaacctgct actattacat
     2341 tagctcctgc ttgtacaact tcggctacgt ttttagggct tataccacca tcaacttcta
     2401 tatcaatgtt taatccttga ctatctataa tagcttttaa ctctttaatc ttatctaaaa
     2461 cacaaggaat aaatgattgt cctccaaatc cagggttaac agacattaat aataccatat
     2521 ctaaatcagg tagtacatgt ttaatcgtat ctactggtgt agctggatta agtactacac
     2581 cagcttttat tccatgtgat tttatatttt gtatagttct atgtagatga acacaagcct
     2641 cctggtgaac agttattata tcacatcctg cttttacaaa ttcttcaata tactggtctg
     2701 gattttctat cataagatga gcatcaaata ccatatttat attttctttc tttaaacttt
     2761 tgactacaag tggtcctaat gtaatgtttg gtacaaagtg tccatccatt acatcgatat
     2821 gaagatattc acatcctgca ctttctactt ttcttacatc ttctaataat tttgcaaaat
     2881 ctgctgataa tattgatggt gctaatttaa tcatgtttct aatacctcct actattattt
     2941 tgtcttattt catgtagcag ctgtatataa ctatcatatc tttctttaga tatttctcca
     3001 tttgtaacag cttcttttat agcacaatta ggttcatttt catgaataca ctttgaacca
     3061 aacttacaat cattaaattt atcaaattct ataaaataat ctttaagttc aacttcctct
     3121 atatcttcaa gggcaaggga actaaaacct ggtgtatctg caaccattcc accaaattct
     3181 aattttaaaa gttcagcgtg acgagtagta tgttttcccc tttttatttt atcacttaca
     3241 actcctgttt gaagtttaaa attttcatct atttcattta ataagcttga ttttccaact
     3301 ccagatggtc ctgcaaacac tactacattt tcttttaatt cttcttttac tttatctata
     3361 tttaacttag taatattgct tacaggaatg actttatagc cactaagttc atatatattt
     3421 ttaacagttt ctaatgtatc attatcatct aaatctgctt ttgtaaggat tattacagtt
     3481 tctaaatttt ctttttctgc caaaactata aatctatcta aaagagataa gtttggttta
     3541 ggatttttta ttgcgaatac tattaatgct ttatctacat ttgcaatagg aggtcttata
     3601 agttctgtat ctctactgtc gatttcttct aagataccct ttttttcatt ttcatcaaca
     3661 atgcttattt tgactctatc accaaccaaa ggtgttattt tctgctttct aaatatccct
     3721 ctagccttac attcataaat accattataa gtatctacat aataaaatcc actaatcccc
     3781 tttataattt ttccttctag catcaaatgc ctccctttat aaaatttata taaacacata
     3841 ctgtttatga gtagtatatc gtgttattct ttcctatttt tatataatac cacatttttt
     3901 ttaaataaca aatcctccgt ttggactaat tacctgacca gtgataaaat ttgatttgtc
     3961 tgatgccaag aaaatagcac aatttgctat atcttctgga gttcctagcc tcattagagg
     4021 ggtttcttcc acaagagcgt ctatatcttc ctcattatat ccagataaca tgtcagtatt
     4081 aatgacacct ggagctatgc tatttactct aatatttgat ggtcctactt ctttagctaa
     4141 tgcctttgtc atacctatta tgcctgcttt tgttatagaa taatgcactt cacaagacgc
     4201 acctgatata ccccagattg aagatatatt aattatattt cctttttttt ctgatatcat
     4261 gtattttaaa gcaacttgtg aacaataaaa gcttcctttt aaattaatat tcatcatatt
     4321 atcccaatct tcatcagtta tatctgtaaa caacttatct tgacttacac cagcattgtt
     4381 tacaagtaca tccaatcctc caaattcttt tatacaataa tctaccatat cctctacatc
     4441 ttctctattt gaaatatcag ccttaaaaag ttttacagaa aaattttttt catttaatat
     4501 agtgtacaac tctttagctt cattttctga cttattaaaa tttactaata cattgtaacc
     4561 ttcttttgca aaagctttag acatagcttt accaatccct ctagctcctc ctgtaattaa
     4621 aactgttttc ttcttcataa atacttttct ccttatatta taaccaattc agtattttat
     4681 acaaaacaaa ctgcccttta aagaaaatat tttctataaa aggacagctc ttattttatt
     4741 aatatttatt ctgttttacc aatgtttgcg ccagttcctg atgaagttcc tccattatct
     4801 cctgagtttg agcctcctga actatctcca ttgtctcctg agtttgaacc tcctgaactt
     4861 cctccattgt ctcctgagtt tgagcctcct gaacttcctc cattatctcc tgagtttgag
     4921 cctcctgagt ttccaccact atttcctgag tttgaacctc ctgagttact tccattattg
     4981 cctgagtttc caccactatt tcctgaattt gagcctcctg aatttccacc actgtttcct
     5041 gagtttgaac caggttctgt tggtgtatta ttctctggtg ttgttggttt tttatctggt
     5101 actgttggag tctgtgtatt tgatttttct gaacctttgc ttacatatag gtttacctca
     5161 tcaccttcct gagctcttga tgagccagca cccggactct gacttaaaac tgtaccttct
     5221 ttatatattg agctgtattc atacttaaca gttcccactt ttaagttatt ttcttcaata
     5281 agttgttttg cattactcaa agtaagtccc actacattag gaacagatgc ctgacttgaa
     5341 cctttactta cgacaacatt tatagtatca ccctttttga catttgtact tccaccttca
     5401 ggtgtctgtg atattataca attttcatca acttcactat tatactcttc actttttaca
     5461 ctcaggtata atccttcttt ttcaacagca ctttgagctt cttctaatgt catatttaca
     5521 agattaggaa cagtcaaaga tttattgcca aaccctccag caaataaaaa cttatatgct
     5581 aagaatactt gagcacacaa tatcagtatc aaaacagccg caattacttt taatcttctt
     5641 ctagattttg ggctttcctg ttttttcttc tttttcttag ttctcttact tggagtactc
     5701 ttaggtctct gattcttttt agctctcatt atctcttcct cttcttcatc ttcatcatca
     5761 tcttcttcat aaaaatcatc atagtaatct tcatcatcat ccaaatcagc tacttcaaca
     5821 ggcttaacca ctttttcagg agctggtttt gcaagtgttg gatttacaac tttattaatt
     5881 tctttttcat caattttctt agttgcaaaa tcatcatatt cttttataaa atctaaatca
     5941 atattttttt ctatatactc tatatcttct atcaattctt cagcagtttg atatctatct
     6001 gcactagatt tttctgtaag cttttttatt gtagttctta cactttgagg aatcctaact
     6061 ttttcttctg atgtaaagtc tatatcatca ttaatgtgtt gaagtgctat agaaattgga
     6121 ctatctcccc taaatggaac ttttcctatc aacatttcat ataaaactat acctagagaa
     6181 tacaggtcag cattattagt tacaaattta cctttagctt gctctggtga aaaataatgc
     6241 actgaaccta ttatactacc aatattagtc attgttgaat ttgaaacagc tttggctata
     6301 ccaaaatcag ccacttttac tactcttcct tcatttgata tgagaatatt atgaggtttt
     6361 atatctctat gtataatccc ctttttgtgc gctgcactaa gtgccatagc tatttgtttt
     6421 gttatatcta atgcagtata ttcatctaat ataccttcat cttgtataat ctcttttaga
     6481 ttcttcccat ctacaaactc cattacaatg tagtgaactt ttccatcttc accaacatca
     6541 taaacattta ctatatttgg atgtgaaaga cttgctactg cttctgcctc tcttttaaac
     6601 tttgttagaa attcatcatc atctacaaat tcaggtctaa gtactttaag tgcaacagtc
     6661 ctgtttaata atctgtcttt agcctcatat acaaaggcca ttcctccatc accaatcttc
     6721 ctgatgattt catatcgatt tcctaaaatt gtatctccca caatatcacc gtcctaatta
     6781 tacttttatc actataaccg aaacattatc tttaccagaa acatcattgg ccatacttat
     6841 caattcttca cttatattac tagttctctc atattcataa tctaatatta aatctcttat
     6901 atcttcatca tcaataaacc cagttaatcc atctgtcgca agaagtatta tatcactttt
     6961 tttgatttct ttcttaaaaa tatctacaac aaccatatca tctgttccaa ttgctctagt
     7021 tattctattc ctctgtggat gtcttcttgc ctcttcttct gtaatgacat gggccatcat
     7081 aagttcttca accacggaat ggtctatagt tattttatca aatttttcat ctgttaacaa
     7141 ataacatctg ctatctccaa catttgccac atataagaca ttattataca ctatagccgt
     7201 aactaatgta gttcccattc cttcaaattc tatatcttcc atagattttt tgtgcacaat
     7261 tgagtttaca ttattataag cctgttttaa tatatcatct atatagtcta tttttacatt
     7321 atcatgttgt aataagtttt cttttagaaa atcaattata ttttctactg ctaatttact
     7381 ggcgacttca ccttttttat gtccacccat tccatcagct atggcaaata ttcctattgg
     7441 accatgttct gtatctataa cttctccctc acaatagtct tcattgtttt ttcttatttt
     7501 tcctatatgg gaggcacaac tataaaccat acgacttcct ccttacaaat tacttgtatt
     7561 ttcttctcaa ttgcccacaa gctccactaa tatcagaacc catagacatc ctaactgtgg
     7621 caggtatatt gttcttctcc aaactatccc taaatttata aataaatgct ttatctggtt
     7681 tttcgtattc tctttcttca actttattta ttggaattaa atttacatga catagcatac
     7741 cttttaggag cttagctaat gcctttgctt ctttttctga gtcatttact ccttttataa
     7801 gagaatattc aaatgtaacc cttctatttg tcttttttat ataatatcta catgcattca
     7861 atatttcttc aatagaataa gcatttgcta caggcataat ttttcttcgt tcttcatcat
     7921 atggtgaatg aagtgataaa gctaaattta tagctatttc taagtctgcc aactcataca
     7981 ttttaggtac tattccacaa gttgaaagag ttatatgtct ataccctata ttaagaccat
     8041 ttttttcgtt aattattttt aaaaattgtt ttgtattttc aaagttatca agtggctcac
     8101 cactccccat caatacaaga tttgataccc tcttaccagt atcttcttga attttaataa
     8161 cttggtctaa aatttcccat ggctctaagt ttctaattaa tccatccatt gtagatgcac
     8221 aaaaattaca tcccatccta caacctactt ggttagaaac acagacagta actctactat
     8281 cataatccat catcacagtt tctattatat ttccatcatc taataaaaac aaatattttt
     8341 ttgtattatc taccttagat tctaacttta attctatatc tatatgccct atacatgaaa
     8401 cctcttctaa tttatttctc aaactttttg gtatattgtt catatcatca aaagtttttg
     8461 cacccttata tatccaagag aatatttgac ttcctctaaa gggcttttca tctatagttt
     8521 tcataaactc tttaagttca tcttcagtaa aattttttaa tactatcttt ttcttttcca
     8581 ttctctcact accttattct ctttaatttt gctatgaaaa aaccatctat accatgaata
     8641 ttaggatata tctttaaata tcctttatct tgattgtcta agtctacatt tactgtatca
     8701 attggaacaa attcaaagtt gtgattttct tcaacaaaag aagtaactat atttatattc
     8761 tccacatctt gaattgtaca tgtactatat actaaagttc caccaacttt gacatattta
     8821 gatgcatttt ctaaaatttt cttttgtata gaagtgatgt cttttaactc ttcttctttc
     8881 ttatacttaa tttctggttt acgtcttatt ataccaaaac cagagcaagg aacatctgct
     8941 aaaacataat caaatttact tatactattt tcatcaatct cagaagcatc aaatctttct
     9001 acaaacacat ttttaagccc caatctattt acagtagagt ttataagctt tagtttatgc
     9061 tcaaaaatat ctctagctac tacttgacca gtattgttca ttaaagtagc taagtgagta
     9121 gatttccctc caggagcact acatacatcc aaaacaaatg aaccttcttt tggattaatt
     9181 actttcccta ctatcataga acttatatct tgaattgtaa ataaaccagc cttaaataat
     9241 tcattttttt ctatattttt cattttttcg actttaatag cttcttctac cataggaact
     9301 tttaaacata caatacccat ttcacttaat ttttcaataa gttcttctct acttatttta
     9361 agtgtattcg ttcttatata gatactaggc ttttcattat ttgcttccaa caaatcttca
     9421 gcaaattctt gtccaaattt atctatccag ttttttataa tccaagagtt ataggaatac
     9481 tttatagata agtattttac actatcttcc tcagttatgt ccattatagt ctctttagct
     9541 cttatttcat ttctaagaat agcatttatg aaaccagaag attttttatc atatttctta
     9601 gataatttta cagtttcatt aactgctgca taatctgata tactatctaa aaataaaatc
     9661 tgatatgttc ccattcttaa aaagatttta acataagttg acattttttt tacctttatt
     9721 tttgaaagtt tattgattat ataatctaaa taatatttat tttctattac tccataaatt
     9781 aactctgttg caagtcctct atccatatca cttatttcta aatttttaaa atgcttattt
     9841 attgcaatat ttgaataatt gtcatttttt tctatatcac ataatacttt aaatccaact
     9901 tctcttgcat ccatataaat ttctcccttt tattaaacat aaaaaagggt tgtttctaaa
     9961 caacccactt tacttttagt catttcttct ttgagctatt agtaacaatc taagtaattg
    10021 aagtattgaa accaatgctg ctgcaacata tgttaaagct gctgctgaaa gtactcttct
    10081 actttgtctt gactcacttt catctataat ccctaaattc ccaagttgaa cgattgctct
    10141 acttgaagca ttaaattcta caggcaatgt tattatttgg aataacactg aagccgaaaa
    10201 caatagtata ccaattttta aaaatggacc agccataata aatcctaaaa atattaaaaa
    10261 ccaagatata cttgaagcaa aattcactac tggtactaaa ctacttctta tttgaagagg
    10321 tgcataacct ttagcatgtt gtatggcatg accacactca tgtgcagcta ctgcaacaga
    10381 agttatagat gttccataat atacatcttc tgacaacctt acagcctttc ttcttgggtc
    10441 ataatggtca cttagatgcc ctctaaccat ttctatcttt acatcataca aaccatttga
    10501 atcaagtact cttcttgctg tttgctctcc agtatatcct cgacgtgtat ttactcttaa
    10561 atacttattt gttgttgaac tcactttaaa ttgtgcatat atagtaagta atatagctgg
    10621 aattaataca accattgttg ggtcaaatcc ccaaaaaccg ccatatacag ggtacataaa
    10681 taacactctc ctaaaatata tttttattat acatcctctt aatattcact tcacttaaaa
    10741 acagtttaat tacaaactaa tatatttttt cctgttaaaa agcctatagc tttttcaata
    10801 tccataatgt ctacattagt atttacacaa ggtccattag gtcttttgtt aaatacacct
    10861 aaaacaggaa ttttcttaat atcttgtatt ccagatgtca aatctctttc acatgcaaca
    10921 gctacaacag ctttaggttt attatccatt ataatttttc tagctagggt tcctccagta
    10981 gctacaaata tttttacatt agttttttcc tttagcttaa ttaattctcc tatgttgcat
    11041 cttccacatt ctttacaatt atctatatca ttagtaactt ttaatttaca actattctct
    11101 tgaatacaat gaggtattaa aattattata tcctctggat taaaattata tttattactg
    11161 tatatatatt cattatttaa ctttatatat attcttctaa tatcactttt tgacatacca
    11221 aatgatgatg caatgaaaga tataatagga aataatccac tgacaatatt aaaatttatt
    11281 ttcattatat ttgagttaac atactttcct ttaattactc tataagtaac tgctgttgaa
    11341 catagtatta taagtattag tgatattatt ataatattta tcatcaagga gaatatttga
    11401 gttaaactta ttataaaaaa attaacaact cctatcaaaa ttcctaatat taaaatcaaa
    11461 atcccaacac ttgttaaata cttctttata tctgtcatac ttttcaccta acccaatact
    11521 aaaccagttt ctatagtatt cccttttata tattcaccaa ccaacattct ctttttattc
    11581 ggcatctgaa tttcttcaat taaaagtaca ttatctatag tacttacttt tatcccatct
    11641 ttgcttacat caattattgt acctacatct tttgtgcttt tttcatctaa cactttagtt
    11701 ttccaaactt tcattatgac atcattatag gtagtatagg cacttggcca tggatttact
    11761 cctctaacta aattgtgtat ttctctggca ctttttgaaa aatctatgtt acctaatgat
    11821 ttattcatta ttggcgcata ggaaaattct tcatgatttt gtacttctct tggagcattt
    11881 ccttcttcaa ttaatcttaa tgtttctttt aaagtttctg cgcctatatt catcatttta
    11941 tcatgaagtt ctcctgctgt tatattttca tcaagattta cttctgtctt tagtatcata
    12001 tctccagtat ctagcccttc atccatatac atagttgtaa caccagtctt ttcttcacca
    12061 tttattatta cccaatttat aggtgccgca cctctatatt ttggaagtaa agaaacatga
    12121 acatttatac atccaagttt aggaatctct aatatttctt ttggaagtat ctgtccaaaa
    12181 gctacaacta ctattacatc tggatttaaa gatttaattt tatctataaa ttctttatct
    12241 ctagccttta ctggttgata tacaggtata ttattttcaa ttgccagttc ttttactgga
    12301 ctcataccta gttttttacc tcttcctttt ggtttatctg gttgagttac aacacccaat
    12361 atttcatact tctcatctat tattttttgt aaacatggaa ctgctatatc aggagttccc
    12421 ataaatacta ttttcatcaa atcaactcct actacttttc tattttgtca acaaacaaaa
    12481 ttccatctaa gtggtctatc tcatgacaaa atgctctagc taaaagttct tctccctcta
    12541 actctattgt ttcaccattt ctatttaaag ctctgacctt tacataattt ggtcttctaa
    12601 catttcccgc ttctccaaca acacttaaac atccttcctc atctatttgc tccccagatg
    12661 tttctattat ttcaggattt ataagttcta taagctcttc tccaatatct attactacta
    12721 ctctttttaa tattccaact tgaggtgcag ccagtccaac accatctgca tcatacatag
    12781 tttctgccat atcatctaat aattgtatta tcttttcatc aattttttca accttttttg
    12841 atttttttct taatactggt tctcctattt gaactatttg tcttaaagcc attataattc
    12901 ctcctaaatt tataatacac tgtttggatt aatatctata gatacattga tattcttatc
    12961 aaatatcaaa tctcgttttg ttatacatat atattttatt atacccttta agagattaat
    13021 ttcaatattt tcatctttaa agagtatttg ccatctataa ttttgattta tttttgatat
    13081 tgaacatgga tttggaccta atataaatcc taaatcattt ataccccttt tttctaataa
    13141 ataaatcaat gatgcatgca tattttttat attttttatc actagtcttt catcttcacc
    13201 actaacaact acacttaaca tattattaaa aggagaatat ccaaatgctt ttcttatttt
    13261 tatctcatct tcataaaaac cctcataatc atattcaata gcatgcttaa tagcatagtg
    13321 ttcagtgtca taagtctgaa gtactacttt tccctcttta tcagctctcc ctgctcttcc
    13381 agagacttga gttatcaatt gaaatgtagt ttcaaaactt ttaaagtctg gaaaatttaa
    13441 tatcatgtct gcagacaaaa tacctactaa agttacattc tcaaaatcca gccctttact
    13501 taacatctgt gttccaataa gtacatctgc ttctttatct ttaaatttat ttagtatctc
    13561 atctaaagca cctttttttg aagttgtatc tttatccatt ctcaaagttt ttatatctgg
    13621 aaaaatatat tttaattcct cttcaatttt ttgagttcca actccaaatg gcttaacata
    13681 agtactttta cattcaggac actcttttgg aatttctttc tcatatccac aatagtggca
    13741 tcttccagta ttgcttttct tatgataagt taaagatata tcacaatttt cacattgaaa
    13801 cacatatcca cactttctac aagatacaaa atttgcataa cctcttctgt ttaaaaatag
    13861 tattacttga ttattttctt caatcgcata acgtatttct ttttgtaatt tcaaactaaa
    13921 tatacttcta tttcctttat ctaactcatc tttcatgtct accacttcaa tgtttggaag
    13981 aggcttatca tttgctctac tttttatatt tataagtttg tattctccag attttgccct
    14041 ataatactct tcaattgagg gcgttgctga acctaaaata agagttatat tattttttaa
    14101 tgccataaat ctagcaactt ctatagcatt aaattttgga ttcttctcag acttatatga
    14161 tgactcatga cattcatcaa ttattattaa gcctaaggaa ttaaaaggtg caaataaagc
    14221 tgacctagct ccaattaaca ctcttacttt acctgctttg acagctttgt aaacatcatg
    14281 tttttctcct tcagatagtt tactgtggaa cactccaacc aaatctccaa acctactctt
    14341 tagcctatct attgtttgag gagttaatgc tatctcaggt actaagaata tactgtctaa
    14401 gccttgattt agagcaaatt ctattatttc catgtaaact tcagtttttc cacttccagt
    14461 tactccatga attaagtatg gttttttatc atctacaaac atattcgact ttatctcatc
    14521 aactgcttct tgttgctcat tgtttaattt tatacttttt agtacggatt tgtaaatact
    14581 ttttggctct ctataataat ccttaaattc tagggttatt agctttttct tacttagaga
    14641 atttatactt tgttttgaag catttaataa atccaataaa tcatttatct ctacattttc
    14701 attattttta agaaaactta atatctcttt ttgtttactt cccacattta ttttattttg
    14761 ctctatataa tcgtcaatct tatcactttc taaactcaaa gatatataac ataccttctt
    14821 ttcatttttg tgatttttat attcccaaca taattttata attccattct tattcattgt
    14881 gtacaaagaa ctatttaagt ttggtatata ttttaactta tcaaccttaa ttttaccttt
    14941 actatcaatc actttctcta ctatttctct atttttatca cttaaagttg atattttttc
    15001 cttcaattca atatcattta atccacttaa actttcacct aataggacta ctttataatt
    15061 atttaatttg tatccctttg gatatatcaa atttatacat tctatataag tacacaaata
    15121 tctatttttc atccaataaa ccaactctaa atcttctttt ctaaatatcg gattttcatc
    15181 taatatatca ataatttctt ttatttttat tttttcatct aagctatctg taaattgaaa
    15241 tacaaaggct tctgttggtt tattaccttt tccaaatggt actaatattc tgtgcccaat
    15301 ttttattaca tcagataaaa atacaggaat ttgatatgta aataaattat ctgtatatat
    15361 agtattactt ctaactatta cctttgcata ttttttcatt ataaaacctc tacttaaatt
    15421 taatatattg ttcatatttt aatatacttt acaagatatt aaaatctcag aatttataag
    15481 caatttcaag ttattaatat taactttatt ttaataatta actattaatt atattttata
    15541 ctttatgaca aaataaatgc tgaaaactac aatttaactg acatttgcga gtttttactc
    15601 gctaaaatta aattgcagct ttcagccact ttagctgtga taaaatacac aaatatcata
    15661 tttaaaaaag gtcttatttc tcaagcaacg ctttaacttt atctaaaata acattggcta
    15721 cttcttcttt tttcatctta ggatactcag taatatttcc ctctacatct attattttta
    15781 caatattagt atcagctcta aaaccagcac cttctttagt taaatcattt gccacaataa
    15841 aatctaaatt tttctttttt atttttcctt tggcatgttc tataaggtca ttagtttcag
    15901 ctgcaaatcc aattagtatc ttatcttctt ttatttttcc tatttcataa gcaatatcat
    15961 aatttctact tagttctatt gataaatcat cattcttctt tttaattttt ttgtcagaat
    16021 aagtttttgg tttataatct gcaactgctg cactttgaat tactatttta ttttcactta
    16081 aatttgatac tactgcttca tacaaatctt gtgctgactc tatttgaaca aattttttaa
    16141 gattttgtgg aatagaaaga tttgtaggac ctgatattaa tgttacatca gcacctctag
    16201 ctatagcttc tcttgctata gaatatccca ttttaccaga agacctatta gttaaatatc
    16261 ttactgggtc tatactctct acagttggac cagctgtaac tatcatactt tgacctttta
    16321 aatcttgctc tttagtaaga ttagtcaaaa cttcctctac tattatatct ggattagcta
    16381 gtttacctgt tccaatatct ccacatgcaa gccttccact ttccggttca ataaatttat
    16441 atccaaagct ctttaaagtt tgtatatttt tttgaacaat tggattttca tacatattag
    16501 tattcatagc tggtgctata agaacctgtg cttttgttgc cataacagta gttgtcaaca
    16561 tatcgtcagc tataccattt gcaatcttgc ctataacatt tgcacttgct ggcactatta
    16621 agaataaatc tgcttttttt gctaatgata tatgttcaac ttcccaagtt tttggttctt
    16681 caaacatatc ttctactaca taattttgac tcagcgattg aaatgtaagt ggtttaacaa
    16741 attctgttgc tgattttgtc attataacat ggatatttgc atttagtttt tttaacttac
    16801 ttacaacatc acaagcctta tatgcagcta taccgccact tatgccaatt acaacagttt
    16861 tatcttttag catgacctta ctcctcttct gaaatttgtt ggtgttgctc agcatgatgt
    16921 ctagcttctt ctatctctat ttcctcttct gtcaataatc tatatgttat ttttccactt
    16981 gcaacttctt tagttgctac acatacaggc ttttccttag ttttatttga aacatatggt
    17041 tcttctccat caattaattt tcttgctctc tttgaaacag tccctactaa atagtatctg
    17101 ttgtctattt tttctagtac ctcattgata gatggtttta acataattat aattcctcct
    17161 taaatttctc tattataata tttttatatc tgctaacttt attcttttca gatgatatta
    17221 taccttctat ttctttagca gatgttttta catcttcatt aaatataaaa taatcataat
    17281 ccttaatcat tttaatttct tcatatgcac agctaaatct cttctctatt tcagcttctg
    17341 tttctgtacc tcttccaaca attctatttt ttaattcttt aagtgatggt ggtagtacaa
    17401 atataaatac tccctctggg caaacttctt ttatttgttt tgctccttgc atttcaatct
    17461 ccagtaatac atcttgtcct ttttctaaac attccattat tgccgctttt ggagttccat
    17521 aaaaattatc ataaatttga gcatactcca aaaactcacc tttttctatc atctcttcaa
    17581 acttttcctt acttatgaag aaataattta ctccatcaac ttctccagtt ctgggttttc
    17641 ttgtagtagc tgatacagaa agttttattg tatcattttc tttaagtagt tctttgcaaa
    17701 tagtaccttt tcctgcacct gatggtcctg atacaactag caataatcct tttttcacaa
    17761 acatttaagc attccccttc taatattgta ttcttactct atattttgta tttgttctct
    17821 tattttttcc aattcgcttt ttacttctac tactaaatta gttatattta tatcagatga
    17881 cttcgaacct atagtgttag tttctctatt catctcttgt attaagaaat ctatttttct
    17941 acctatagag tcgtctttaa atatagtatt tttaagttgc cctatatgac ttttaaatct
    18001 aactatttct tctgttatac tgcttttatc tgcatatatt gcaacttctt gtgctaatct
    18061 attctcatct attatacttg ggtcatccag taattcactt attctcaaat tcaatttttc
    18121 tctataatct tctacaacac tacttgaata tttttcaatc tcttctatat gattttttaa
    18181 taaatcacat ctcatggcaa tatcttcagc taatttttta ccttcttcac ttctcatttc
    18241 tcttaatttg attaatgcat cttcaactgc ttgattcaac attgaccaca ataaatcttc
    18301 atcttcttcc ttttcttcta ttttcacaat atcaggaaac tttgcaacat tcataacgct
    18361 aatatcatct ataatatcaa atttattttt tatttctttt agtatgtcta tatactgaga
    18421 tgctaatccc tcatcaaaat ttaagtttac atcttctttg cctaataagt caaactttat
    18481 gtataaatct actcttcctc tttttacata attttttact aaatttctaa ctttatcctc
    18541 taaaaatgaa atttttcttg gaagacgtat gtttatatct gaatacttat gatttatcgt
    18601 tttacactct actaaaaagt aataattatc atccttgtat tctcctcttc caaaaccagt
    18661 catacttata gccattaatt aaacctccaa aataccttca catattttta ctgctggacc
    18721 tgtcatataa gagaaatctc ctacatctat tttaactgtt ccaccttgag acgttacatt
    18781 tacactactt tctaccatat taagtaaatt gcacactatt gctgatgcag tcataccagt
    18841 accacaacct aaagtatatc cacaacctct ttcccaagta taaacatgta tattatactt
    18901 atcttctacc tttacaaaat ttacattcgt tttttcagga aatattttat tattttcaat
    18961 ctcttttcca tatttacaaa catcatctac atctaattca tctactataa ctactgtatg
    19021 aggaaccccc atcaacatag aagtaacttt aagctcccta tctaaaacct ttatactttc
    19081 ttgcacaaaa cgttctttat ttgtaagtac aggaacgtcc ttagctataa aacttccttt
    19141 tcccatattg actcttatag agtttatttc tctattagta agatttactt ttatatcttt
    19201 tataccatct aatgtatcta ctgtaaaact ttctttttta actatacaat tatcataaac
    19261 aaatttacaa aaacatctaa ggccatttcc acacatagct gctcttgaac catcagaatt
    19321 ataatatacc atttctacat ctgcaacttt tgaatctttc acaactaaca atccatccgc
    19381 tcctacagaa aatcttctat ggcatacttt ttttgctaaa gcagtataat cagatggatt
    19441 tatctggtcg aatcttccat ctattgctat aaaatcattt cctattccat gtagtttcca
    19501 aaatttcata agtatcgctc cttcgtaaat taacacttaa actattataa cataaaaaaa
    19561 ttcccataaa cttataattt atgggaaaag ttttgtaatg acaactataa tttgaggatg
    19621 tgagggagag ttgccattac aattagggta attatattat gtttttaagc tagttctttc
    19681 tatgtttata tattaatatt tctatatgaa ttttatgtat gttttattta aactgtctat
    19741 gaatttttta attaaacatc tggtaactag ctataatcct tctagtagat atctattaac
    19801 atacacctat taattcttta agtgttttta aaacatagtc taaatcttcc ttagtaatat
    19861 ctacatgagt tacaaatcta tattcaccat tttccatttt attaattttt atatttcttt
    19921 caaaaaattc ttttattaaa acatcctctt ttataatatc ttcactcatc ttaaaaaata
    19981 ccatacttat atctggatta acattgttta cttcaatgcc atcaatttta tttaattcac
    20041 aagctaaata ttttgcattt tcatggtctt cattcaatct taaagtcatt ttttcaatgg
    20101 caattatacc agctgctgca actataccta cttgtctcat tcctccaccc ataacttttt
    20161 gctttcttct agcttttgct ataaattctt tatttccagc aagtatagaa cccattggtg
    20221 cagctaaacc ttttgacaca cagaacataa cactatcaca acactttgct atttctttag
    20281 cttctacacc caaagttaat gctgcgttaa ataatcttgc tccatctaaa tgtactggta
    20341 agttatgttt ctcagctatt tttttaattt ctcttaaatt atcaagactc acaactgttc
    20401 cacctgaata tgcattttct acacatataa gccctgtctg tggttcatgt atgtcatctc
    20461 ttctaattgc cttttcaaca agttcaggat ttagagctcc actttcgtct tcatctatag
    20521 ttctaagctg aactccagct attacagcag gtgccccaac ctcatgagtc acaatgtgat
    20581 aacctttacc aataattatt tcttgccctc ttaggcaatg agtaaataga gctaattgat
    20641 tcccaaaagt tccacttgga aggaaaatag catcttcttt tccaacttta gctgctgcta
    20701 attcttcaag tttatttaca gtagggtcat ctctaaagat tgaatctcca acaggagcct
    20761 ttgccatagc ttctctcatt tcgtcagtag gcatagtaac agtatcactt cttaaatcga
    20821 taaatctcat tttttcaacc ttctttcata tttatatact atagaacctt aaaattgatt
    20881 ttttctgtaa agttcatata ctttcatagc ttatactcta cacaataatt atactatatt
    20941 tactttatat ttaatctact ttaaattatt aattttaaaa ataaagtttc ctctctaata
    21001 aatataaata attatacaat ttaaatatta atctctattg tttaaattcc ccctagtcac
    21061 aaagcgtata ttatgtgtat aattaattta ctgactcatt ttggtcaaag aaaggggtgc
    21121 tataaatggc attagatgga ttagttatac attcaatagt cgatgaatta tcatcaaaac
    21181 ttacaggtgg aaaaatagat aagatacatc aacctgaaga tgatgaggta atttttaata
    21241 taagaaataa taaagaaaat tttagattgg ttttaagtgc aagtgcctca aatcctagag
    21301 tttatcttac aagtaattat caaaaagaaa accctctaaa agcgcctatg ttttgtatgt
    21361 tacttagaaa atacatacaa ggtggaaata tagtagaaat ttctcaaata ggttttgaaa
    21421 gaataataaa aataagtgtt gaatctttag atgaattaaa agaaaagaca gttaaaaata
    21481 ttatgattga aattatggga agacatagca acataataat aactcatggt gaagaaaata
    21541 aaattataga ttctataaaa agagtccctt ttagcataag tagagttcgt caagttttac
    21601 ctggtcatga ttattcattg ccaccagaac aaaacaaact caaccctctt gatgatatat
    21661 ctaaggattt gtttattaaa aacctagagg aattggaagg acctatcttt aagtctatat
    21721 attctaaatt tctaggtata agccctatta tagctaaaga aatttgctac agagcaggtg
    21781 taaatcaaaa tgctatcata aaggatatat ctgatgaaca atttgattct ttacacaaag
    21841 tattttgtaa tttattcaat gatataaatt ctaacaaata ctctccttgc attattatag
    21901 ataaaaaggt ggataaggta gttgatttca gttgtataaa ccttacatta tttagcgatt
    21961 tatcatatat aaataaggat agtatgtctc gtattctgga agatttctat agaactaagg
    22021 atattaaaga tagaattaat caacgttcat ctgatttaaa gaagagtata tctgttaagc
    22081 ttgatagact ttataataaa cttaaaaaac aagaagaaga attatcagaa tccgaaaatg
    22141 cagacattta taagataaaa ggagaattga ttacttcata tatttatatg gtagaaaaag
    22201 gtatggaaag tattgaagtt gctaacttct atgatgaaaa ttgtaatgat gtaacaatag
    22261 aattaaataa aaacttgaca ccatctgaaa atgctcaaaa atactttaaa aagtataaca
    22321 aaatgaaaca tgctaaagtc gagatatctc atcaaatttc cttaaataag gaagaaatag
    22381 attacttaga aaacataatt ttaagtattg aaaactgtga aaatttagct gaattacaag
    22441 atataaaaga agaacttgct aaggttggat acatcaagac tcaaaagaaa aacagtaaaa
    22501 aggatactat cccatctaca aaacctcatg aatttgtttc ttctgatgga tttaaaatat
    22561 tggttggtaa aaacaataag caaaatgact atcttacact aagacttgct gataatgatg
    22621 acttatggat gcatacaaag aatattcctg gttctcatgt aataataaag tgtgctggta
    22681 aggaagtccc tgataatact gtatttgaag gagctatgct agcagcattc tttagtaagt
    22741 caaaactttc ttctcaggtg cctgtagact atactaagag aaaaaatgtg aaaaaaccta
    22801 gtggttctaa accaggtatg gttatttatg aaactaacag tactatttat gttactcctg
    22861 aagaagaaac agtagccaag cttaaggtta aatctgaata aattctgcac taaatacaaa
    22921 ataaatgtta gacaactaca cattatcatt ctaaataaaa atagtggcct tcttataaag
    22981 tacaataata aggccacttt ctaattagct tacttttaat tttctaacaa aatacttttc
    23041 tgttttaata actatactta tattcccaac aaatattttc agtcttctta tatagatgta
    23101 attaaaaagt taacactaaa agctattttt taatttattg atttgtttta gtaattcatt
    23161 atattctgat tcatacaatt ctttttttag attatctttt tctatagata acatagatat
    23221 aacagttgta agtctatttt ctaatactag aagattttct tttttgattt ggttttctct
    23281 actaggagtt tttttatttc tgctaattgt atattcgtca taattacaat caaactccct
    23341 tattttagca tcttttatct ctataatata gttacaaata tgtgatacaa atactctatc
    23401 atgagacact ataagcatag ttttatcagt atttatcagt gccttttcca gtgcttccat
    23461 agattttata tctaagtaat ttgttggttc atcaaatact aaaaaattat tatcttccaa
    23521 tattatttta catagtgcta tttttacctt ttctccacca ctaagtactt ttactttttt
    23581 gtagacatca tcacctttaa acccaaatag gcttaagttt atccttataa aagattcatc
    23641 aaatgaacta ttaactttag tattgtatag aacactcttt tcctcatcta ataaactttg
    23701 attttggtca aaataaccta cttttacctt gttatttatt tttatattgt catttttatc
    23761 agccaatatt tcttttatca gagtactttt tccacaccca ttatcaccta ataatgctat
    23821 ctttttacct ctttttatct taaaggaaac attatctaac aggagcttat tttctaactt
    23881 tagagtcata tcttttacct ctactagatt tttactaatt atctccatac catcttgaat
    23941 cttaatgttc atttctttag aaactttagg tttattcttt acctcaagtt tatcaattct
    24001 gctttttata gccttcacat ttgagtccaa ctttttctta ttagtctgac cacccatttt
    24061 gtgcaaccta gcttctgaat tacccattct ttttggtgtt tttcttatgc catcacttaa
    24121 agctttttta tataaaagag ctttttcaag cctttttttc tctttaacat attcttgata
    24181 ctcaaaatca gctcttttta cttcttcatc tttttgcatt aaataatcag tgtaattgcc
    24241 tttgtaagct tttagttttc catcttctag ttctaatata gttgtacata actcatccag
    24301 aaaacgtcta tcatgagata ttaataataa tgctccctca tgccttttaa acatatcttc
    24361 caaaacccca atgctttttt tatctaagtt tgaagttggc tcatcagcta ttatgatatt
    24421 tttcttatta ctcagcgcat ctgctatttt taacttaacc ttttcccctc ctgataagtg
    24481 ttcttcaaat ttatctggag catttaaaag actcttctcc ttactatgac tacatgtttc
    24541 aatattattt tcactctgac ttatataaga atagctttct gtaaggtaag tatatccttc
    24601 atcaatttct atttctccca atattgcttt taataatgtt gtttttcctt gaccatttga
    24661 accaactaga cctattttgt cattttctaa tatttcaagt ttatctatat ccaaaattaa
    24721 tttatctgca taatatttct ttaaattctc tacttttact aacaacataa aaaatccctc
    24781 cttaacacac ttaaatataa ggagagattc tataaatagg cataacaaat aaacgtatag
    24841 actataatct actctcctta atttaagcat tgcaaataaa gccaccaaaa taggcttttc
    24901 aaaaattaca atactttaaa ttataaaaaa tagattatat tctcattgtc ctagttacct
    24961 tgccctttct ttcatttatt tttaatcatt atatcaacat taattacaaa tgtctataga
    25021 aaattaacta attttagtta ttcaatatac aacttattct ttaaatttat tcttcttcaa
    25081 ctttaaagtc ctgattattt aaaattctgt ttaatacaat tccaacaatt gcagctaaac
    25141 taagaccagt tatctttact gtacttgtta tagggattcc aacagaaata cctttattag
    25201 ataaatatgt tgttccaaga cctattaata atataactgc tatgattaat atatttattt
    25261 tactttctac acaagaactg tcccttatag tttttaatcc tacataagtt atcatcgaaa
    25321 atagcattat actaattcca cccataactg aacctggtat actttgaaga aatccaccaa
    25381 acttgcctac acatgcaagc aatatagcaa atattgctgt acgtctaagt attgaagggt
    25441 catagttttt agttatagca agtactgctg tattttctcc ataagttgta tttgctggtc
    25501 ctcctaaaca ccctgcaact atagtcgcaa atccatcccc aagtaatgtt ctatttaatc
    25561 caggattttc tataaagttt ttacctacta cagcaccatt agtagtcata tctcctatat
    25621 gttccataaa tacagctaat acaactggag atataatcac tattgctcct aaactgaact
    25681 taggaactgt aaagttaggt attgcaaata aacttgcttc tgtaatagta gttatatcta
    25741 ctaatcttaa tatcaaacag attatatacc cagaaaaaac tgctattaat attcctaatt
    25801 gttttataaa tcctctacca aacttattgg ttaaaattgc tattgctaaa gttataaatg
    25861 ctatcacaaa attggctgaa gccatactaa aagctgttgg caataaattt aatcctataa
    25921 ctactatcat agctcctgta acttgtgcag gaaaatataa tttaatttta tttacaccaa
    25981 ctacttttac tataatcgac attataatat aaattaatcc tgctactatt ataccacctt
    26041 gagcatatgc taaatctcca ccatacgcct ctttagctgc tagtataact ggtataaacg
    26101 caaaacttga acctaaaaag gcaggtactt ttccttctgt acaaaaatga aatatcaatg
    26161 taccaacacc agcacagaaa attgcaactg acggattaaa acctgtcaaa atgggaacta
    26221 atactgtcgc tccaaacatg gctaatagat gttgtaaaga cattatcgtt tttttcataa
    26281 aaacctccaa aattattata tataaataaa tcctctgttt aattaatacc ttttaattaa
    26341 ttatcattcc ctagattcta ttttttaacc tctatatact tattatataa tttatcatta
    26401 ttttacattt tacgacatca aaaactcaaa taaaggagaa taaaaactac attaagactc
    26461 acatccataa aattataata tttattttgt gcaatctatg taagctttat cctcctccct
    26521 gactattcaa tgctaagctt ttatataata cttaaaaata attattcttt gattgtaact
    26581 gaatcttctc catcaatttc taataaactg acagatataa tttcatgtct agaagtaggt
    26641 acattttttc ctacataatc tgctcttata ggcaactctc tatgccctct gtccactagc
    26701 actgctaatt gtattgattt aggtcttcct atatctataa tagcatctag tgaagctctc
    26761 acagttcttc cagtgtataa aacatcatca actaaaacta ctgtcttatc atttacatta
    26821 aaatctaaat aagttccttt aactacaggt tcaacatgaa ttttttccaa atcatctcta
    26881 tacaaagtta tatcaatatc tcctgtatct acttttgttc cttctatttg ttctattttc
    26941 ttagaaattc tatttgcaat tggcacacct ctagtcttta ttccaactaa aactagattt
    27001 tctacacctt tatttctttc aattatttca tgacttattc tagtaattgc tcttgctatt
    27061 gccttttcat ccattaattg agctttttct accatatcat aattattgtc aaagatataa
    27121 tatatctatt tgacaaatct actcctttct tttaattttt tataaattta tttttattta
    27181 aaatttcaat ttaaacaaca aaaaaacttc tcatcacaag acaagaagct cttatataaa
    27241 aaatatacag tgtaatttag caattgtctt acgatatctc tgtatcgttt aaagataatt
    27301 ttttaatcta tcaactcaaa ttatatattt atatttcaaa tttttcaagc ttttttaaac
    27361 aaaatacttt aatataagat attttattta aatttcacta aatttattta cttatataaa
    27421 aacaaaaaat gatttataag tattatacaa atggttattt tatcttttta ataacttcct
    27481 taaaatactc aggtaattct gaattaaatt cgatatattc cttagtagta ggatgtataa
    27541 atcccaactt cttagcgtgc aaagtttgtc cttttaattt aaatttagtt tcttttggac
    27601 catatacata atcacctaaa agtgggtgat ttatagacaa agcatgaact cttatttgat
    27661 gagttctacc agtttcaagc ctagctctca tatgtgtaaa cttatcaaat ctttctacaa
    27721 cttcaaagtg agtgattgca ttttttccat cttttactac agccattttt aatctatcct
    27781 taggatttct ccctataggt ttatcaacag ttattttatc ttctttaacc acaccatagc
    27841 atataaattc atactcccta gttatagtat gttcctttaa ctgttctgct agaaaattat
    27901 gtgcataatt gttcttagct atcataagaa gaccagaagt atctttgtct attctatgaa
    27961 ctataccagg tctaataact ccatttattg atgacaactt atctttacaa tgatataaaa
    28021 tcccatttac taacgtattt tcatagtttc caggagcagg atggacaacc atatcctgag
    28081 gtttatttat tattaagaca tcatcatctt catatacaat atctattgga atatcttgag
    28141 gtaaaacttc taatacttta ggttctggta tctctataac aatattatct tcctctttaa
    28201 ccagatatct agctttttct actttattat taacctttac ttttccatct tttataattt
    28261 tttgtatata acttcttgac atatcaccta attgttctgc tagataaata tcaagtctaa
    28321 cttcttcttc ctcttcaagt actaaaaact cttttacttc atccattata tcacctactt
    28381 ttactttcaa aaaataaaac atatatacat aaaaacacag ttcctacaac tacaaataca
    28441 tcagctacat tgaatacata ttcccatata actctaaagt cgaagtaatc taccacaaaa
    28501 cctaatcgta ctctatcaac tagatttccc aatgcaccag atataattaa tataattccc
    28561 aaccttccaa atatatgtac tttccttgta ttaagatagt ataatccaaa taccgttgca
    28621 agtaatgcaa caattataaa tatccattga ttattttgta ataatccaaa tgctgcacct
    28681 ctattttcga cataagttaa atgaaataca ttatttatta ttggtattgt tgatacatcc
    28741 accaaattat tcaataccca tattttagac agttggtcta aacctatgag tagaattatt
    28801 attaatatat atagcaatga tttttccccc tataaattat ttaaaaacca attttatttg
    28861 aagcacacta ttattattat acaaaaaaaa tgctctaaag agcaacttta ttaaaattgt
    28921 attttcttaa taaagctaga acaaaattta aaatagtact acttttttta tattgtagat
    28981 ttctacatat ttaaaatcat atcattatgt ataaaaaatt taaaaatacc atactaatca
    29041 atattttttg tgtattttat agattaaaga ttaatgatgt ctgtaattta aacttacaat
    29101 caccactaat cttcaaacaa atatttttac ttttgcactt gcttattttt atctaaatgt
    29161 tgtataagtt cagatactgt tacgaattta tatcctctct cttttaattt aggaattact
    29221 acttctaaag cttgtatagt ttgtgattta tcatttcttt tagtgttata atcatgtaaa
    29281 agtattatat taccattttg gacctttgta aggatagtat ttataattgc atctactcca
    29341 gggttagacc aatctctagg gtctaagttt gaccaaagaa ttatattcat tcctttagaa
    29401 actataatat cacagacact tttactcatg gctctatatg gaggtctaaa tatctttggt
    29461 tctactccta tcacactttt tataatattt tgcgtatctg ttatttcttt ttcaacacta
    29521 ctataactat tttttgcaac atttatatgt gtaaatgtat gattccctat ttcatgcccc
    29581 tcatcatatt gtctttttaa taattctttg ttatattcta ctttttctcc aactacaaaa
    29641 aaagttgctt ttacatcatt tttctttaat atatcaagta cttgtggtgt aaaatcctca
    29701 tctggaccat catcaaaagt caatgctaaa attttttcat cctcatttcc tttttttatt
    29761 attacatctt cataatcgtc ttttatattg tcatttaaac tatatgcttc ttttgaacta
    29821 tcttgattat gtgtattatg attatatgta tatacaccta ctatagataa taaacatata
    29881 catataccac atattatttt cttcatgtca ttacctcttt tctaattcat tctagcaata
    29941 tattttataa tgtcagtttt ttaatctctc aatattccat ttttatttaa atcagttaaa
    30001 tttgttaggt cttctgaata aaagttagaa tcttctacat cagggttttg cattgcatca
    30061 cctaataaat tattttcctt ctttgcacaa tcattacata aacttgtatc tggcaatata
    30121 tccaatcttt ctaaatctat ttgattatga cataaatcac aaatcccata tgttccattc
    30181 tctattttag ataatgctaa gtctatttga tacaatcttc cctcttcatg atttattaga
    30241 ctgttttgca tattttgcat atataaatct gttcccatgt ctcctatatg attatcataa
    30301 ctagataatt ctcctgatga atacttttca cttgtatgtt tagttgtatc tccaaataaa
    30361 gtattatctt tcatatcctc tactaaatta gttaaatttt ctctttcttt caataattta
    30421 tctttatatt ttttagtatc catttttaac acctctaatt ttaatattta catcataaaa
    30481 atagtatgtg gaaaagtaaa ttaaacatac aataaaaata agatttgatt cgaaaaatgg
    30541 aatgtctcaa aatgaaattt ttagttcatt taaccctctt ttttatatga aatcaaatat
    30601 atttttatcc tttcagcaat aaaaaagaga cttatctcta ttttgagata gtctcttctt
    30661 tgaacataat ttaaatacaa ttttttaatt tttatagtta ttattttact aacttgccaa
    30721 tttatgatta ttttgttctt taatatgttt tgctttttta aagaataaaa gaccaagaat
    30781 tattgctaca ataacaccta ttccattaga tatattaact ggtagtctaa atccttcttt
    30841 tgcttgcatt atataactaa atgttactac agtcataaaa agtgctggta atgtagttat
    30901 ataatggttt ttatttgctt tgactaaata tgcagaacca gtccacaaca ttatcattgc
    30961 aagtgtttga tttgcccaag agaaatatct ccaaattata ttaaaatcta taaatgtaag
    31021 agcaacacca actgcgaata atggtaaagc tattaaaaat ctatttttga ctgcatcttg
    31081 attataattc attgcatctg ctattgtcag cctagcactt ctaaatgctg tatctccaga
    31141 agttatagga catgccacaa ctccaagtat agctaaagct cctccaatag gccccattaa
    31201 tccaacagat actgtattta ctactgttgc tgctccacca tcattaaata tcacattaag
    31261 ttgaggtatg ccatggaaat atgacatggc tgctgctgcc caaactaaag ctattatacc
    31321 ttctgcaacc attgacccat aaaatactgg tcttgcctca ctctctttct caacacatct
    31381 cgccataagt ggtgattgag ttgcatgaaa acctgatatt gctccacatg ctattgttat
    31441 aaataagtat gggaataaag gtgttccatt tggattcata ttttgtaaat ttatatttgg
    31501 tatgttatac ccctttatta ttaatcctcc agctacacct actgccataa ttaataatgc
    31561 tgctccaaat aaaggatata ttttaccaat caatttatct attggtaaaa cagtagccat
    31621 aatataataa agtattatca ctccaaccca tatttgtgcg tttacacctg tcaatgtctt
    31681 taataatcct gctggacctg ttacaaatac aactccaacc aatactaaaa gaataacact
    31741 aaatattctc attgctattt ttgcaccatt tcctagataa aggcctacca actctgatgc
    31801 agatgtacct ttgtttttca ttgatagata tcctgaagca aaatcgtgta ctccaccagc
    31861 aaagattgtt ccaaacacaa tccataaaaa tgctgatggt ccaaataatg ctccttgtat
    31921 agctccaaat atcggaccta accctgctat atttagaaac tgaattaaaa agattctctt
    31981 ccatttcatt ggcacataat ctactccatc cctacaagct atagctggag tttgattttt
    32041 gtcattggtt ccaaaaacct tttctacaaa agtaccatag acaaaataac ctactataag
    32101 tactactatt gaacctaaaa agcttaccat aaattcatcc ccttagtttt tatataattg
    32161 ctttataaat ttttatgagt ttaagaaaac tatttccagt tcaaatagta atactttttt
    32221 atgctaatat aaatattaat tacattaagt gcatatttaa gtacatgaaa tgactttata
    32281 tatgcattaa cagcctaaat tcttttactt tgcttctact tacagttact tcataatcac
    32341 tattttttaa ttttaatatg tatgtattat taaaccatgg tataatttct tttactttgg
    32401 ttaagtttac gatataagac ctatgtgttc taaaaaatgt tttatcattt ataagatttt
    32461 cgacttctta tatgccttct tttaatataa acttttcttt ctcagtatat ataaatgtct
    32521 gcctttcatt agcttcacag taatatatat catcaatatc tattacaact aatttatcac
    32581 ctttccaaag gctaatttta tgtgttattt cttctatctc ctgatttact gcttccttct
    32641 tatatttgta taaattacta tttacattaa atagttcaat atcactcatc tcactttttt
    32701 ctaatttatt cagcatagaa ataattctct cttcagaata tggtttcaat atataatcaa
    32761 atacatccaa agaaaaagca tctactgcat aactttcata tgctgttata aaaacaattt
    32821 taggtcttga tttaaactga tttaatgttt ttgcaagtag cattccatca agatgaggta
    32881 tatttatatc taaaaaaatt acatctattt tattttcctg aataaagtct aacacctcta
    32941 taccattggt aaattcactt actacctcga ttccactttt attttcaata aaatatctca
    33001 attcctttct tgctggaaat tcatcttcca ctattatagc tctcatactt ctactccttt
    33061 acataaaata ctatcaaagt cccattatct attcttctta tattaagccc tcttccatac
    33121 atcagtttaa gccttaaatg tacattataa agcccaatct tattttctgg catattatct
    33181 tgatatacat tatttataat ttcctcactt atacctatac cattatcctc tattgatatt
    33241 ctcacagtat tttctaatga atacggtaac ttttttatgg atattttcac tacaccagct
    33301 ctaccacttt ctaaaatacc atgtattatt gcattttcca ctaatggttg tataatcaaa
    33361 cttggaatct ttatatttac atcttctata tcataagata cagttagtaa ttctccaaac
    33421 ctagcttttt ctatctctac aaaagattta acttgttcaa tctctttatt tatatcaatt
    33481 aagttatcac tatattcaag attatatctt agataagttg ataaatttat tattaactct
    33541 ctagccttat ctggactaaa tcttataaat gaagctattg tgtttaggga attaaacaaa
    33601 aaatgtggat taatttgtct ttgaagtgct tttatttctg ctttatttct cgcttctata
    33661 tcttttttat ctttctcaat cccctctact atagatataa caaatcctat tccaatctgc
    33721 ccaactatta aaggtaaata tattccaccg attatttgaa ttgctaaaga atgtggatat
    33781 gatattaagt atataagtaa tatagtaaaa ctttcagaaa tcatgcctac caatattcca
    33841 tacattactc tatgttgttt tggtatcctc ttatatagaa atcctgataa tacacctgct
    33901 agtatacttg ataaaagaca tggaacagat gttatccccc caatatccat aaaatatcta
    33961 tgaatccctg aaaaaactcc tgctgtaata cctaccatcg gaccaaataa aatgccacct
    34021 gatactatag atattattct agtgtttact attgacccca tataatttat accactataa
    34081 gttccaaata tggctaaaaa agtaaaaact aatgctatac agaccaagtc attaaaacta
    34141 tattcttcct tttgaaatat ctgcttaaat atttttaact ttgttattag aagaaacaat
    34201 actacaataa ggcttgtctt ttctattaat gcgattaaaa ttgatgtttt cataatcccc
    34261 ctttaaaaaa taaattttta cacactctaa atttattatg gttgttataa attacatatt
    34321 tgaagtaatc ctatcacaaa actttccaca tttaatcttt taattaaata agaaatagtt
    34381 gtacttctat taacaactaa tactagctag cataagcttg taataacttg tagttattat
    34441 taactttaca attctagtat ttaattttat actttagaaa tctcttttct tagaaataat
    34501 actttcgaat atcatataat acaatattat gtagattctt actcatacaa catatttctt
    34561 taaaaatttt atactattct ttgttcaaat atctcatttg atttaattgt gttaaacaca
    34621 cattatcatc tctctgttat atatttatta tatagccgta tatcgataaa gtaaataaat
    34681 cccatattta aaataatatg tttgacaata aattattata atgtatattt accataataa
    34741 tttgtcaacc ttgaaagtta cttctataat tattgttaaa taattcttct aaaaaaaata
    34801 cctata
//
LOCUS       DAMJVK010000038        33815 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.038, whole genome shotgun sequence.
ACCESSION   DAMJVK010000038 DAMJVK010000000
VERSION     DAMJVK010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33815
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.038"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            718..1569
                     /locus_tag="PC414_001892"
     CDS             718..1569
                     /locus_tag="PC414_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HDF2464288.1"
                     /translation="MEINKKHFIQVFLTCLILVVICEFIGQRTIAIGKASIVLFPMLY
                     AVIIGLIITPDLLGKKIKALKKVVGEKEINIAGEVVGIALVMLGIKYGTTVGPNIDKI
                     IQAGPAFIAQEFGHILAPIVALPLALMFGMKREAVGATASISREPSLGVISEKYGINS
                     PEGSGVLGTYLMGTVLGTIYFSVLGSISIYSGLHPYALGMACGVGSGSMMTAAAGSLS
                     TMVPPEMADTILAYAATSNMMSSITGIIFLVFVSLPFTNFMYKILEPKFSRNKKTKEI
                     KSTEGEC"
     gene            1573..2043
                     /locus_tag="PC414_001893"
     CDS             1573..2043
                     /locus_tag="PC414_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464289.1"
                     /translation="MFANLNLKKSSYQLLVMSVIGVIILIGQRISVGTSIVTALPGMV
                     MLILAAMAAMIIKDLFPKSIFPAFGFATIIGLLLSMPYSPTSEVFLTNTNNINFMAIT
                     TPLLAFAGISVGNKIEALKEMSWKIVIISLIVFTTIFFACASIAHIVLSIQGKI"
     gene            2056..3366
                     /locus_tag="PC414_001894"
     CDS             2056..3366
                     /locus_tag="PC414_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2464290.1"
                     /translation="MNKEQLKSLVIKTIDENREKILKIGQGIYKNPEYGYKEFKTTEA
                     VSNFFEQELNLSVEKNIAVTGCKADANSSKKGPHISILGELDGISCKEHKDANEIGAS
                     HTCGHNIQIAGMLGAAVGLVKSGVLEHLDGKVSFMATPAEEFIELEYREQLKKEGKIT
                     YFGGKQELIKRGYFDDIDISMMFHASDLGEDKALVGPESNGFVGKKVKFIGKESHAGS
                     APHDGINALNAAMLAINNVNALRETFKESERVRFHPIITKGGDIVNVVPADVHMESYV
                     RARTINGMIDANERINKALMAGGMAVGADVEITEIPGYLPILRYKSLDNLFKNNLEDL
                     GLKGKVIDGGDFTGSFDFGDVSHIMPTLHPMIGGVKGALHTRDFEIVDEDLAYIIPAK
                     AMALTVVDLLFDNAKDAKDILDTFTPVMTKEEYLTFLEKHDKTL"
     gene            3578..4324
                     /locus_tag="PC414_001895"
     CDS             3578..4324
                     /locus_tag="PC414_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2464291.1"
                     /translation="MFNAGNRIINTWLYPIQDGFVLIDTGYENGFKHFKKRISKFNIK
                     IKDIRYIFLTHAHDDHVGFLNQILDESPNTKIVMHDKALEILYKGQNSFIGGCTSRTA
                     FLFCQIMKLFGKGEHSFSPLKQEFEKRCILVSDKNRKEIEKELNGKIIDTPGHTDDSI
                     SLLIDEGVLFCGDSAMNGFPSTHKITIFAESKLEFIQSWKTIINTKPKMIYPGHGKPF
                     EYSKLEQNLIYVNKINLYPLHNSKDIHRFL"
     gene            4686..6227
                     /locus_tag="PC414_001896"
     CDS             4686..6227
                     /locus_tag="PC414_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HDF2464292.1"
                     /translation="MNDNVVKKKNFFDRTLNRIETVGNKLPDPVTIFLGLCVLLLILS
                     SLVGSMGISVVHPGTGETITAVNLLTVEQLQILLGNIVSNFQGFAPLGLVLVTMIGAG
                     VCDKTGLMTATIKASVSKIPETRVTLVVMTIGMLANIASDAGTILFPPLAALVYLGVG
                     RHPLIGLFSGYAAVCLGFAANIMISVNDILAASFTVPAAQMIDSGFQANATMNLFFMI
                     ASTFVLILLATWVTEKIVAPRFGKYEGNAELDVDGSLSDVEKKGLKKASISILIYVAI
                     IVALSVIGKKPFLADPETGDLLSNNAPLMKGMVPIIALAFFIPGVVYGKTIGKIKKDK
                     DVVSIMASAMSDMGGYIILAFAASQFLQLFTNSNLGLILAVKGGEFLKSAGINGPLLL
                     IGFIILSCFINLFIGSASAKWAILAPIFVPMFMMVGYNPALTQMAYRIGDCITNPLSP
                     LFPYFPIILAFTRKYDKDAGMGTVIANMIPYSISFLIVWTILLILFMVFNIPLGPGIL
                     PSYSM"
     gene            6262..7368
                     /locus_tag="PC414_001897"
     CDS             6262..7368
                     /locus_tag="PC414_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDF2464293.1"
                     /translation="MLDKNRLINNFMDMVRIDSPSNQELEMSKWLVNYLKERNIDAII
                     DDAGEKYGGNTGNVIAYIKGEEGSRPLCLCAHMDQVQPCLGVKPILDGNVVRSDGTTT
                     LGADDKAGIAAILEALEHVITEKIPHRDIYLCFTICEEAGMHGVKNFNPDNLPCKDMV
                     ILDSGGAIGSIAYKAPAQQSIKISFQGKKAHAGIEPEKGLNAILVASHAISNMHIGRI
                     DSLTTSNIGEIEGGGATNIVTDKVTLTAEIRSHIPETLEYELNHMEKCCKDAASKFNT
                     TYTFEHNMSYPSFELSRDSHVFKLSEEAIRQVGVVPNPMVIGGGSDANILANLGYNCA
                     ILSLGMYDVHTVNEYVNIDELYDTAKIVYHMIKL"
     gene            complement(7747..9033)
                     /locus_tag="PC414_001898"
     CDS             complement(7747..9033)
                     /locus_tag="PC414_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF2464294.1"
                     /translation="MIVGIIGPSDSGFKIKDDLKQIDSDLKTKIYVREKVVDTIEVIS
                     KCEDECDAIIFTGCAVYEFIKSKYEISKPHSFVPRSGTSIMKAFWAIKSANIKLDKFS
                     IDVVDRYVVEDALKEFDIKATSVFCNPFSLEVGESELVEWHIKLFEENKTDIMLTGFG
                     AVYNELKERGYPVFRLQATIQLIKESYDKVKSEYALSKARFSQIAVEILSLIDYKEKI
                     DNYYSDMIKKSDMDKLVVNYVRSIQGSLFLLGRNDYVVFVHKGVADNEYNYDKLFNLK
                     REIKDMGFSLSIGIGTGVTAYQAENNAHKALRHSIDSKEIGIYLVDEDENIRGPLASE
                     NELNYSLMLSDEKVIEISKKTGMSCESVAKIIAINENRKSKIYDSKELAEYLNVSERS
                     ARRILNKIVNAGLGRICAKETSIGGGRPKNITEILF"
     gene            complement(9156..12254)
                     /locus_tag="PC414_001899"
     CDS             complement(9156..12254)
                     /locus_tag="PC414_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HDF2464295.1"
                     /translation="MDVINLIDELDEIVYVSSLDTYELLYINEIGKKAMGLNDISHLK
                     CYKALQGKDSPCEFCTNSILKEDEYYIWENTNTRTNRHFILKDKLIKWEGKHARLEIA
                     LDITEKEDISKSIAEKLEIKELLVGCIKHLITESNFSDAVNLVLTNIGKFHKADRAYV
                     FEVSEDRKKVTNTYEWCNEGVDEQKQALTNLDINELKNWFLLFEQKGFVIIDDIENIK
                     EEQPIEYEILKPQKIESLMTSSLKQDDKVVGFIGVDNPHKAVGDTSLLTSLSYFLLTE
                     MKKRKVENKLNYMSYYDFLTGAYNRNKYIKYIDNFQKGKVKSIGVVFVDINGLKDIND
                     NFGHANGDSAIVSACVIIKKYFHSYNVYRIGGDEFVVLCEDISKQLFEELVSWLSEEF
                     KDEMIYSVAVGSIWSDDNIDIHKLIKVADERMYQDKKRYYNKNKLRNNEMKVISDLAD
                     STNNILFKHKELVDKVYFDDLMIKGYSKAYEKLAYNTYDIILSICIDTNLITPIYQSE
                     SNIFSFPIKENLEKTFSYIKKHLIHPDDIILFNDNHLLKSLDSLNNSKQDDLELNFEY
                     RRLGIDKKYYWALIYMLKISSKNKLGEKQTHILVAIKNIDYLMQLHKNQKDTLTGLYN
                     YESFCEQISLMLKRYPDKRYAIIMMDIDKFKVINDIYGVRGGDKALIYVAELIQKNIG
                     SNGICCRMYADVFCIFYESYSDRDIHKVIYKIKNSLNRKKFEYMLVPCFGVYRILDNH
                     IPITNACEMAGYAHKKVKKQSINNYIFYDDTIRNDALEEKQMEREMEYALENGQFHVY
                     LQPKYNLNANKIVGAEALVRWVHPEKGIIPPIKFISLFEKNGFIIRLDMYVWEQVCSL
                     IRKWIDLGEEPVPISVNVSRLHLHNPHFKEIVLSLINKYSIPKSFLELELTESLFIKN
                     IKLFTKIIADLRMDGIVLNMDDFGSAYSSLNMLKNINIDTLKIDCGFFNEEGITDREK
                     IVLKHTIAMAKDLDMDVVAEGVETKEQAEFLVTLDCVVIQGYYYCKPIPSADFEKLLY
                     QK"
     gene            12980..13138
                     /locus_tag="PC414_001900"
     CDS             12980..13138
                     /locus_tag="PC414_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464296.1"
                     /translation="MKIFSFLLFVIGICLCIAGFIGHYPVFVGTTLSFLAIAMIAVRQ
                     VKTKKKMK"
     gene            complement(13425..14129)
                     /locus_tag="PC414_001901"
     CDS             complement(13425..14129)
                     /locus_tag="PC414_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2464297.1"
                     /translation="MEIKPLVLIVEDDKPICKFIKVSLETQNYRCVETDNGGTAISLI
                     HSLDPDLIILDLGLPDIDGIEVIGRVRACAKTNKIIVVSAREHERDKVEALDGGADDY
                     LTKPFSVTELLARVRVALRNKAQQDNINNDAPKSFEVKNLKIDYENHIVSINGEEIHL
                     TPIEYKIIELMSKYSGRVLTHKFIIDKVWGNYYESENQSLRVFMASIRRKIEKNPAQP
                     EYILTEVGVGYRMADE"
     gene            complement(14120..15727)
                     /locus_tag="PC414_001902"
     CDS             complement(14120..15727)
                     /locus_tag="PC414_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HDF2464298.1"
                     /translation="MKKERLQHEDLKEYDYDEDGYLSKKIIIKDFIKTILVMTIATGI
                     SVVFEKVGFNESNIILVYILGVLFATSITYGYFFGILSSVIGVLSFNFFFTHPLYTFL
                     AYRKDYPMTFCIMLMAAIITSTLTSRIKREVRLSHKREKMIRLLYENNKTLLKAKNKN
                     QIIEFCGSNLVNILNRTVIVTIADENNNLGKPSIYICNNDGSENIFHSVLEKEAIKEA
                     FRTGNPVGIGTKYCKNSSAYYFPIKGQESNVLGVIGVACFKIDIITENEKILLEAVST
                     QVALAIDRENLFEKNKKANLEAERERLRGNLLRSISHDLRTPLTSILGSSSTILENDD
                     VIDKETRVELLKNIYEDTSWLTHSVENILSMTRIDEGKLDIEKRPEVVDEIIAESILR
                     VKKFANSRDIKTNIPDEIIIVHVDVLLIEQVLVNLIDNAIRHTPKNSKIELTVKKESN
                     QVLFEVADNGKGIPNEDIGNIFNRFYTKNKSRNLERRGIGLGLEICKSIVEAHGGEIV
                     AFNNPSGGATFRFSIPMYDEEGKDKWK"
     gene            complement(15981..17159)
                     /locus_tag="PC414_001903"
     CDS             complement(15981..17159)
                     /locus_tag="PC414_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464299.1"
                     /translation="MEKLSDKRRVKAVSMETFVFIVLLAVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLDTVFLIMAMAVLAGALSALLSEFGVISLVNKIFKGLMRPIWGLPGASIA
                     GVVATYLSDNPAIIPFAKDKTFTQYFKKYQVPALCNLGTAFGMGLIVTTFMIAQGKEY
                     VLPAIIGNVAAIIGSIISVRIMLTFTKKYYNYDPKNDTEKQINDKGAKLEEFREIRDG
                     NVFQRTLDAILEGGKLGVEMGMAIIPGVLVVCTLVMLLTFGPSTDPATGQAVYTGAAY
                     EGIKLLPAIGDKISFIIEPLFGFTSPEAIAFPVTALGAVGAAISLVPEFIKSGAITPN
                     DIAVFTAMGMCWSGYLSTHIGMMDALDARPLAGKAILSHTIGGLCAGICAHFIFMLVG
                     "
     gene            complement(17385..17879)
                     /locus_tag="PC414_001904"
     CDS             complement(17385..17879)
                     /locus_tag="PC414_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDF2464300.1"
                     /translation="MHLFIEVIAAFFTALSFGVLFNMKGKNLILAGIGGSIAWFSYKF
                     LLNIGVTENLCFFIATVCFAIYCELCARIYMTPATTLSVCCLIILVPGYGIYNTMYSV
                     LTNNYIKAVEYGVSTLSCASSIALGLVFITTVFRKVNLYGVLTKIKENEKYKKSINKI
                     KQQK"
     gene            complement(17880..18641)
                     /locus_tag="PC414_001905"
     CDS             complement(17880..18641)
                     /locus_tag="PC414_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDF2464301.1"
                     /translation="MDIDRILNFSSNAGKAMLQSGGETYRVEETIARICQSFGIDHVD
                     VFATPTAVMASVFVDGKLHSAIKRISSRRVDLNLVHEVNSLSRAISINNLDIELCEKI
                     LDEISEDNYYSDLITIFFAGIAAATFSILFGGNVEEFIAAFTVGILTKFVIMYLSKSS
                     LNDFFVNAIGAVIIASCSILILKLGFIKTLDHLIAGAIMLLVPGLALTNALRDLLDGQ
                     LISGLAKLAEVFFVGVSIAVGMFLVLGLYFKLGGI"
     gene            19343..22177
                     /locus_tag="PC414_001906"
     CDS             19343..22177
                     /locus_tag="PC414_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2464302.1"
                     /translation="MLRKDKVLLSLHKLCENMTLKDLQSDKTGFTTIEIANHSSLDRS
                     NTSKELNNLYNEKKVLKISGKPILFLHASIIENLIGRTLNSDEFSINNCNELLKSSKN
                     DSEDIFSTLLGHDSSLKLPIEKAKSAILYPPKGLNTLLIGPTGVGKSTFAETMYKYAV
                     ESKIFSSESKFVVFNCAEYAENANLLLSNLFGYVKGSFTGANKDKFGIVAQADGGILF
                     LDEIHRLPPEGQEMLFLLMDKGIYRRLGESDIMHKANVFIVCATTEDIHSSLLGTFLR
                     RIPVTINLPALQDRSLKERLSLIKYFFIIESKYTNATIKVHKDVIKSLLLYDCFGNIG
                     QLKSDIQLMSARAFLNYKTGLKDNIEIDLSLVPDYIYIGLLKLNENRSNLNKLSELDY
                     MLFYEFNGNNLEVEYTKELSKYNIDTSNNGFKYSTEIPYLENDIRSMFINYMEKYSTK
                     LLSISSVTHSDYTSNEIFKVINPKIYYAVEHSLKEAEKISRNKYTKQTYIALSMHISA
                     LIENINQDTYNPKYNLYNDKIFLDKDLNIAKIVLSIIENDLQTSLPKDEVKFISMFLN
                     SMYLEPNNQSESIGVIILAHGNSTASSMCDVANSLLGTNHCNAIDMPLDVKVSTILDK
                     TIELVKECDEGKGVLLLTDMGSLIAFGELISEKTKIKTRSIEMVSTPIVLEAVRKSTL
                     PEMTLDELANSLSSLNPYIGRAAIDSTEINYPEKSREYVIITTCITGYGSAMKVADFI
                     NSSLKNISKYNINLVPHNIESFKMYKNKFSNTKILAVVGSVDFNVANTPFIHIEELLN
                     GYGLDMLNKIIEGTFNANSITNIKETNLSYNSLIINFMTQNLELLDSVKLFNYVNSSL
                     FTIIELIDENLKSKFKLGYILHCSFMIERCFKNTTFPYNNITELISKNHELYSLIREA
                     FKPTEAYFNILIPDEEIAYIIDMINEYLNK"
     gene            22220..23464
                     /locus_tag="PC414_001907"
     CDS             22220..23464
                     /locus_tag="PC414_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDF2464303.1"
                     /translation="MEDEILKGKIKQLTILALIFIFITPVFAFADTPPVPNSSRAALL
                     IDQETKRILFEKNIDEKMPLASLSKMMTFLLAIEAVDKNQVKETDMVKIDKSTASVGG
                     STCKLKDGDEISLGELMQGLMLVSGNDAAIAIAKHIGKTEKNFVNMMNKKAEEIGMID
                     TYYFNPNGLPIYTDPEHKEPPIENMSTAHDIVTLGKYMYDHYENQVTRITTMQVYNDT
                     KKDFTHYNTNPLLVSVPGVDGIKTGYTDNAGYCLAFSMMVPKDAKNERNHRLIGVVLG
                     DGNKKNRISSSATLLKYGKDNFHSKKIAHKGDIIETPCVDGIDDFKITVKVDKDLYGV
                     VSDNENINPKVVFKNMNYPIHKGDIVGVAKYYNDSGKFVGSVDVKSESNIGCIPLKDK
                     IKIKVAKINKKLEIKNSVCFKA"
     gene            complement(23879..25621)
                     /locus_tag="PC414_001908"
     CDS             complement(23879..25621)
                     /locus_tag="PC414_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2464304.1"
                     /translation="MKKTINNNLFRILNIIVYKNKTKLIVSFIMVFIIAGVNIVIPQL
                     IRCIIDDGIGNKNIFLLINLILIYIFIRILSATFDVILSYLYTSIKNKVTINLKVKLL
                     NHISKLSGRYYSSKKTGNILSIMESDIFMIENFGAEIIFDIIVNVFTAIISLIFLINL
                     QFDLFLVVLFLQLILTFSQNKMTKLISKKISEIRSDSGSLSNLIQEYVSNIMDIVILN
                     SKKNFFNKYLKRERDLIKKDLNLNIIMSSNIAISQILTGLITILIYGIGGYKIINNKM
                     TMGELIAFQQYTGMLIGPCIAIVRANTRIAQSKVSINRVFSVIDEKIDIKVDNNGYRI
                     DNSEIKKIDFKNVSFRYGEDLNLVLNDINLQFEKGEVTGIVGGSGCGKSTITNLIYRL
                     WDINEGDILIDGVNIKDINLKSLRNNIDIVTQDQFVFDDTIKNNIVLNNKISDKELDE
                     ILEVTCLDDFIKNLDKGLDEVVGEKGVKISGGQKQRISIARALVSNKKVLILDEATSA
                     LDNISQKEILNNLKEYIQDKIVIIIAHRLSTIKSADNIYVLDKGRVIESGNDAELLHK
                     DGLYNKLVETADFA"
     gene            complement(25605..26279)
                     /locus_tag="PC414_001909"
     CDS             complement(25605..26279)
                     /locus_tag="PC414_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2464305.1"
                     /translation="MKILKVKDLKKIYGKYENEVRALDGIDLDIEPHKFTAIVGASGS
                     GKSTLLHCVAGLDKPTLGNVYLDDVDLYTLNDDKLSEIRRQEFGFIFQSYNLIPVINV
                     YDNIVLPVLLDGKKEDKEYVMRLINKLGIEKQIKKYPNELSGGQQQRVAIARALANRP
                     SVIFADEPTGNLDSKTTNEVMNMLEWCVKEYKQTLVMITHNDNIAKTADRIITIKDGK
                     IDEKNY"
     gene            complement(26316..27305)
                     /locus_tag="PC414_001910"
     CDS             complement(26316..27305)
                     /locus_tag="PC414_001910"
                     /inference="COORDINATES: protein motif:HMM:NF012311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HDF2464306.1"
                     /translation="MTKVRIAVLDTGLDVNDDDINGNYVYDSNFQLKDLGGKEINDIN
                     GHGTFVSKTILNISKNTQIYPINIFNDSGKTSSLNLLSTLSKLTMSDIQIINISAASI
                     NSMYEKELEKVCNILNSQGRFIICSKHNKYEDKESIPTIFESVIGVVGNENIYKNNQY
                     IYEPENKIQMYANSKDNFIKFKDKVTHFGKNSKACAVATGIVANILKNNPYIKFNELE
                     EILIQKSKSKEDLKIENLNTPSYKSNLIRLDIARKIINVINRNFSKIKIDFEFVSEYS
                     LFNNFTCIGNYNAYNFLTILNKEFNISIDYKEVYLYQLENLDMAVDLIYENLI"
     gene            complement(27353..28597)
                     /locus_tag="PC414_001911"
     CDS             complement(27353..28597)
                     /locus_tag="PC414_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2464307.1"
                     /translation="MNSSNKYFLDCICEKIINNNQVILSNKVNGRWVKIPIECYEVLK
                     YSVDNALQLGNLYDVFQENEDKKYYERVIDKLEHIGLLSDSILSEFRLERINKISFSP
                     TNRCNLSCDYCCVDSKIDNNDYLDTSQVRKVIDNIVKLNPRILTITGGEPLMRNDFLE
                     LLDYIKEKFKGKIILSTNATLIKEKQIDDIINGLYAIEISLDGYDELSCSEVRGKGVF
                     CKVLNVIRLIKLKKFENVGLSMVVGKNNEKDIEKFNKLNEDLGTTPIIRNFMNIGRGN
                     ENYSKYLNDKLDIDYICKDDYVDESSLCSNMCKAGVNQISIDHKGDVYPCPNLEYKDL
                     KIFNILEFNENILERILNREMDIFNIFDKLKPVNIESCKECKVNLFCTTCPSKIYTLK
                     NNKKMFDANCKKMKNILEPILW"
     gene            complement(28639..31185)
                     /locus_tag="PC414_001912"
     CDS             complement(28639..31185)
                     /locus_tag="PC414_001912"
                     /inference="COORDINATES: protein motif:HMM:NF014717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2464308.1"
                     /translation="MINIKLAVSYLKRQKGKTLSLILSIGIAVMLVFSLSVINESQGQ
                     SDINHAYKNSGDYHAFYEDINSDIVKELNGEEDIKEFNDVLKFGEIISKDNGASLNLC
                     SYNKDFLDSTRYKLVKGREPRTSNELVIEQKVLDKMELDVKLNQTIDFQIVNEHINKN
                     NENSIYSKDKQFKIVGILSKSDEYYENLNKYKVRAFTYSNNGKDIVPYELITHMGTLK
                     LNTKDLDSQKVVNLGRKYKLTSNQFYPNTEVSMSMYMKNISKKTTYSIKQSLLPLLCA
                     TLVIYNMFNIIILDMTKQIGLLRAVGASKRNIRQVFFIQSIVVLSVGIVIGLLGGIVF
                     SYLGLMIVYDKSAELTINTNSIIESIIMSIIAVILASVVPIYKAGNLSIIDAIKKTDK
                     LNYIPRLLLLKSHKKVVGIINEIAFKNIFRNKVTSIIIIITISLCGVLFIGRLTSTKF
                     IYGKESDSANITSKSYGNFDIYLGYSPINANYTFSKYDNSLLNEIKAIEDVTDVEPNI
                     YLKGYLRNADLEEDYLDELKRTEINNNNESTVLIRGYNKELLNNIDKYIDKGENVYSY
                     TDRDYKNVLLVNNFYSRIKLSNNTQIIKSPKIGDLIDIKIPVYKDGEYKYINTKVRIA
                     GIMKNSYISEQDGESQSGGIQIIFNEKDLKELTGISDYNKVFVKIKKETDKKVIKEIN
                     NIIEKSGFSDIEGRYVRNHFGDHYNKSSYKLAMICVFGVLLISSINIIFIVRSNIIVR
                     LSEICTFRAIGMSKKNVKRMLIKENMIYGVLSMIVAGIISSFNYYKIVSETNKLNQQV
                     YGINNSIKFEIPIYEILIFGSISVFVCVIAVYFSNKMINKLSIVSGIKEN"
     gene            complement(31393..31554)
                     /locus_tag="PC414_001913"
     CDS             complement(31393..31554)
                     /locus_tag="PC414_001913"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464309.1"
                     /translation="MIHIKKIEEKIELQDVEVQADPCKWYYDKHGIISQIDCMVDCAG
                     STPKGTVAY"
     gene            complement(31610..32626)
                     /locus_tag="PC414_001914"
     CDS             complement(31610..32626)
                     /locus_tag="PC414_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2464310.1"
                     /translation="MKFTIFVTEDCNLRCTYCYEGNDKKKENMSYETADKTIDFICNK
                     ELESNSDYTTIVLHGGEPFINFDLIKYIHRDLVDRLSDKKILFDITTNGTIINDEIIE
                     FIAKNVKNISISIDGKKNIHDINRIDANGVGTYEKVMENGKRLIDKGIYVRARMTYNS
                     NTVKYLYESVYDIAKQGFKNIAPISDFMDKNWDNSHIKILNEQISKIMNIKKDYPDID
                     ISILDKQFINKKKGDCFGGINSYAIDTRGDVYPCTVCVGDKDILLGNIYDGLDDKKIK
                     NLIKIYISKNEECDGCTRLDYCMSTRCKLINKKVTGKFQSAVPIYCGLERVYVETTKK
                     LCSL"
     gene            complement(32623..>33815)
                     /locus_tag="PC414_001915"
     CDS             complement(32623..>33815)
                     /locus_tag="PC414_001915"
                     /inference="COORDINATES: protein
                     motif:HMM:NF015983.1,HMM:TIGR04085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2464311.1"
                     /translation="SEEIYKYFLLGQENKIKIYDFVNYFDDESDKKYIINLIDIMNKN
                     QIFKSNNLNFDMLSNGVAIYFCLTNRCNLFCKHCCVESSSNKKDLLSTYDLKNIVDII
                     SVLNTPHIIITGGEPLIREDFFEIIKYIKQKLPDTKLILSTNGTLIDEHNVDFIITNF
                     EKIDISIDGVDENTCSETRGKGVFNKVISSVNLLKDNGFSNIKLSMVFGQKNSHLNKQ
                     FKDLNKKLGTEFVERYFIPTGRGLENKESYLIEDTVLPITIPYILDNELKMCKDKKNK
                     KMGCCACNAGNSQIFINYDGDVYPCPSLIKDDYKMGNIFDNEFVKVIKENKTRDLKSL
                     IKLESIYPYNFEKCKDCDVNIFCWHCPAILDSVKDNELELDRWCSMMKHNLNTAVWGD
                     EKVN"
BASE COUNT        11655 a         5314 c         3917 g        12929 t
ORIGIN      
        1 tacttaattt acttatttct atatcaattc acttattatt acctcacttg ttatcatctt
       61 acttaattta cttatttcta tatcaattta cttattatca tctcatttgt tatcgtctta
      121 cttaatttac ttattgctat gtcaattcat ttattattat ctcacttgtt atcgtcttac
      181 ttaatttact tatttctata tcaattcact tattatcatc ttaattcatt tgcttattat
      241 catttacttt attcaatttc tatatcaaaa ttagtatttg tacttaatac ttaaaattaa
      301 tttaaagtct ttacatatta tttattttct attgtaatat tcatttccat actttactaa
      361 gctacttcca ctcatttatt ttaaaatttc aatttactat ttctcaactc tatattatta
      421 tttttcaatt ttatattact aattttaaag ctttttagct attattccta caatatttaa
      481 acaatttttt cataataaag ctatattaca atatattcaa aatatgtaat attttagata
      541 tttttcgttg acaatgttaa atttttgttt tatccttaat taaggacatg ttattgacat
      601 ttctgaaact tatttgaaat agttttttca ttttatttag gaaaacgtat tatacttaag
      661 aaaacgtatt ttctcatttc agataagtaa aatatttatt cttttgggga ggaaatcatg
      721 gaaattaata aaaaacattt tattcaagta tttttaactt gcttaatctt ggtagtaata
      781 tgtgaattta tagggcaaag aactatagct attggaaaag cctctatagt actgttccct
      841 atgctttatg cagttataat tggacttata ataacaccag atttattagg taaaaaaatt
      901 aaagcactta aaaaagtggt tggagaaaaa gaaataaata ttgctggtga agttgttgga
      961 attgcactag taatgcttgg tataaaatat ggtactacag ttggaccaaa cattgacaaa
     1021 ataattcaag ctggaccagc gtttatcgct caagaatttg gtcatatact tgcaccaatc
     1081 gttgcattac ctcttgcatt aatgtttgga atgaaaagag aagctgttgg agctactgca
     1141 agtataagtc gtgaaccttc tttaggagtt atatcagaaa aatatgggat aaactcacca
     1201 gaaggtagtg gtgtacttgg tacatattta atgggaacag ttcttggtac tatatacttt
     1261 agtgtactag gttcaatatc tatttatagt ggtcttcacc catatgcttt gggaatggcc
     1321 tgtggtgttg gtagtggaag tatgatgaca gcagctgctg gttcattatc taccatggta
     1381 ccacctgaaa tggcagatac aatacttgcc tatgctgcaa caagcaatat gatgtctagt
     1441 ataacaggaa ttatattctt agtttttgta tccttaccat ttactaattt tatgtataag
     1501 attttagaac ctaaattttc gagaaataaa aaaactaaag aaattaaatc tacagaagga
     1561 gagtgctaat aaatgtttgc taatttaaat ttaaagaaaa gttcatatca attattggtt
     1621 atgtctgtta ttggagttat aattcttata ggacaaagaa ttagcgttgg tacatctata
     1681 gttacagctc ttcctggtat ggtgatgcta atattagctg ctatggcagc tatgattata
     1741 aaagatttat ttcctaaatc aatctttcca gcttttggat ttgcaactat aatcggcctt
     1801 ttacttagta tgccatatag tccaacatct gaagtattct taactaacac taataatatt
     1861 aactttatgg ctataactac tccactacta gcttttgcag gaatttctgt tggaaacaaa
     1921 atagaagctt taaaagaaat gtcatggaaa atagttataa tttcacttat tgtatttaca
     1981 actatattct ttgcatgtgc gagtatagct catatagttt taagtattca aggtaaaata
     2041 taggaggaat ttattatgaa taaagaacaa ttaaaatctc tagtaataaa aactatagat
     2101 gaaaatagag aaaaaatatt aaaaataggt caaggaatat acaaaaatcc agaatatgga
     2161 tataaagaat ttaaaactac agaagctgtt tcaaactttt ttgagcaaga attaaattta
     2221 tctgtagaaa aaaatatagc agttacagga tgcaaagcag atgcaaattc tagcaagaaa
     2281 ggtcctcata tatctatact tggagaattg gatggtatat catgtaaaga acataaagat
     2341 gctaatgaaa taggtgcttc tcatacttgt ggacataata ttcaaatagc tggaatgtta
     2401 ggtgctgctg ttggtttagt aaaaagtggc gttttagaac atttagatgg taaagtatct
     2461 tttatggcta ctcctgctga agagtttatt gagttggaat acagagagca attaaaaaaa
     2521 gaaggaaaaa taacttattt tggtggaaaa caagaattaa taaaaagagg ttattttgat
     2581 gatatagaca tatctatgat gtttcatgct tctgatttag gagaagataa ggctctagtt
     2641 ggtcctgaga gtaatggatt tgttggaaaa aaggttaaat ttataggtaa agaatctcat
     2701 gctggttctg ctccacatga tggaataaat gctttaaatg cagcaatgct tgctataaac
     2761 aacgtgaatg cacttagaga aacttttaaa gagtctgaaa gagttagatt ccaccctatc
     2821 atcacaaaag gtggagatat agtaaatgtt gttcctgctg atgttcatat ggaatcatat
     2881 gttagagcta gaactataaa tggtatgatt gatgctaatg aaagaataaa taaggctttg
     2941 atggctggtg gaatggcagt tggagctgat gttgagataa ctgaaattcc tggatatctt
     3001 ccaatattac gatacaaaag tcttgataat ttatttaaaa acaatcttga agatttaggt
     3061 ctaaaaggaa aagttattga tggtggagat tttacaggct cttttgattt tggagatgtg
     3121 tctcatataa tgcctactct tcaccctatg attggtggtg taaaaggagc tcttcataca
     3181 agagattttg aaatagttga tgaagattta gcttacataa ttcctgctaa agcaatggca
     3241 cttacagttg tagaccttct ttttgataat gctaaggatg ctaaagatat attagatacc
     3301 tttactccag ttatgactaa agaagaatat ctaacattct tagaaaaaca cgataaaact
     3361 ttataagtct atttttataa tagggtggta tctactgtaa aagttatacc actctttgtt
     3421 tttgtaacag tatacttctt ttcttttagt ttttagatta aactaaaata tattatcctc
     3481 cttaaaactt ttagagctac gttgaaatat cagtagaact atgctatatt ctttctaatt
     3541 aatataaatt tctattctaa ttcaaggtgg tgaaaaaatg tttaatgcag gaaatcgaat
     3601 tataaataca tggttgtacc ctattcaaga tggctttgtc ttgattgata ctggatatga
     3661 aaatggtttt aaacatttca aaaaacgaat ttctaaattc aacataaaaa tcaaagatat
     3721 tcgatacata tttctaactc atgctcatga tgaccatgtt ggttttctaa atcagattct
     3781 agacgaatct cctaatacaa agattgttat gcatgataaa gctttagaaa ttctatataa
     3841 aggtcaaaac tcttttattg gtggttgtac cagccgtact gcctttctct tttgccaaat
     3901 tatgaaacta tttggaaaag gtgagcatag tttttctcca cttaaacaag aatttgaaaa
     3961 aaggtgtatt cttgtgtctg acaagaatcg taaagaaatt gaaaaagaac taaatggaaa
     4021 aattatagat acaccaggac atactgatga ttcaatttca ctactaattg atgaaggtgt
     4081 tttattttgt ggagattctg caatgaatgg atttccaagt actcacaaaa ttaccatatt
     4141 tgcagaaagc aaactagaat ttattcaatc ttggaaaaca atcatcaata caaaacctaa
     4201 aatgatttat cctggacatg gaaaaccatt tgaatacagt aaattagaac aaaatctaat
     4261 ctatgtaaat aaaataaatt tatatcctct acataattca aaagatattc acagattttt
     4321 gtagaaaata caaagtaaat ttatttgatt aattaaaaaa tctaacactt tactacgcta
     4381 cttattttaa taacttaatc ttccactagc ttttcataaa attttgtttt aaaattaatt
     4441 ttaaagtgca tatgttattt aaatacatta gataaaattc gtcattaatc tacttaaagc
     4501 atatttaact aacaccactc aaccagaaaa ttcagaaaac tgcattgaca caggaaaaag
     4561 tttattttat acttttttta ggacatattg tggacacgtc ttaaatcgac aaacagaatt
     4621 taaattgtaa gattaaccac aatacttatt ttttaattat atttttaaac attagggagg
     4681 atattatgaa tgataacgta gttaagaaga aaaacttttt tgacaggaca ttaaacagaa
     4741 tagaaactgt tggtaataaa ctaccagacc cagttacaat atttttaggg ttatgtgtac
     4801 ttttacttat actatctagt ctagttggtt ctatgggtat atcagtagtt catcctggta
     4861 ctggagaaac tataaccgct gtaaatttat tgactgtaga acagcttcaa attcttttag
     4921 gaaacatagt cagcaatttc caagggtttg ctcctttggg tctagttcta gtaactatga
     4981 taggtgctgg tgtatgtgac aaaacaggac ttatgacagc aacaatcaaa gcatcagtaa
     5041 gcaagattcc tgaaacaaga gtaactcttg ttgtaatgac tataggtatg cttgccaata
     5101 tagcttcaga tgcaggtaca atattatttc caccattagc tgcacttgta tatcttggtg
     5161 ttggtagaca tccacttata ggtttatttt ctggatatgc agcagtctgt ctaggatttg
     5221 ccgcaaacat tatgataagt gtcaatgata tacttgctgc aagttttaca gttccagcag
     5281 ctcaaatgat tgattctgga tttcaagcca atgcaacaat gaacttattt tttatgatag
     5341 catcaacttt tgttctaata cttttggcaa catgggtaac agaaaaaatt gtagctccta
     5401 gatttggtaa atatgaagga aatgcagaac tagatgttga tggaagtctt agtgatgtag
     5461 aaaagaaagg gcttaaaaaa gctagtataa gtatattaat atatgtggct attatagtag
     5521 ctcttagtgt aatcggtaaa aaaccattct tagctgaccc agaaacagga gatttactat
     5581 caaacaatgc tcctcttatg aaaggtatgg tacctataat agcactagca tttttcatac
     5641 ctggtgttgt atacggaaaa actataggta aaataaagaa ggataaggat gtagtctcta
     5701 taatggctag tgcaatgagt gatatgggtg gatatataat actagcattt gctgcttcac
     5761 aatttttgca attattcaca aatagtaatt taggtctgat actagctgta aagggcggag
     5821 aatttttaaa atcagcagga ataaatggac ctttattgtt gataggattt attatattat
     5881 catgttttat aaatttattt ataggtagtg catctgcaaa gtgggctatt cttgcaccta
     5941 tatttgtacc aatgtttatg atggttggat acaatccagc acttactcag atggcttatc
     6001 gtataggtga ttgtataaca aatccactta gcccattatt tccgtacttc cctattatac
     6061 tagcattcac aagaaaatac gataaagatg ctggtatggg tactgtaatt gctaatatga
     6121 taccatactc tatatcattc ttaatagtgt ggactatact attaatattg tttatggtat
     6181 ttaatattcc actaggacct ggaatacttc cttcatatag catgtaattt atttaaatct
     6241 ttctaaaagg agaaataatt tatgttagat aaaaatagac ttataaataa ttttatggat
     6301 atggttagaa ttgatagccc atctaatcaa gaattagaaa tgtctaagtg gttggtaaac
     6361 tacttaaaag aaagaaatat agatgccatt atagatgatg caggtgaaaa atatggtggt
     6421 aatacaggta atgtcattgc ctatataaaa ggtgaagaag gaagtagacc actttgttta
     6481 tgtgcacaca tggaccaagt tcaaccttgt ttaggtgtaa aaccaatact agatggtaat
     6541 gtagttagaa gtgatggaac tactacactt ggtgcagatg ataaagctgg aatagctgct
     6601 atacttgaag ctttagaaca tgttattaca gaaaaaatac cacatagaga tatttatctt
     6661 tgttttacaa tctgtgagga agctggtatg catggtgtca aaaactttaa tcctgacaac
     6721 ttgccttgta aagatatggt gattttagat tctggtggag ctattggttc gatagcatac
     6781 aaagctcctg ctcaacaatc tataaaaatc tcttttcaag gaaagaaagc acatgctggt
     6841 atagaacctg aaaaagggtt aaatgctata ttagtagcct ctcatgcaat aagcaacatg
     6901 cacataggca gaatagattc attgactact tcaaatatag gtgaaataga aggtggtgga
     6961 gctacaaaca tagtaactga taaggttaca ttgactgctg aaattaggtc tcatatacct
     7021 gaaactttag agtatgagct taatcatatg gaaaaatgtt gtaaggacgc tgcatctaaa
     7081 tttaatacta cttatacttt tgaacataat atgtcttacc ctagttttga acttagtaga
     7141 gatagtcatg ttttcaagtt aagtgaagaa gcaataagac aagttggtgt tgttccaaat
     7201 ccaatggtaa ttggtggtgg aagtgacgca aatatattag ctaatcttgg atataattgt
     7261 gctatattaa gtctaggtat gtatgatgta cacacagtta atgaatatgt aaatatagac
     7321 gaattatatg atactgccaa aatagtttat catatgatta aattataaat atcataatag
     7381 aactctttaa attataaaaa taaaaaccta tgtatcctca actttataag ttatatatta
     7441 gagttgtttt acataggttt tagtattttt atataataat ttgttttaac atttttcata
     7501 ataagctttg atattttata tagtaatttg ttctaacatt tttcataata agcttcaata
     7561 ttttgcataa taatttattc tagcattttt aataataggc tctagtattt tatattaata
     7621 atttattcta gtattttaca taattatttg ttctaatttt tttcataata agctttaata
     7681 ctttatatag taatttgttg tagaatttta tacaatataa tgatttatac agttttataa
     7741 aatatcttaa aacagtattt cagtaatatt ttttggtctt cctccaccaa tactagtctc
     7801 ttttgcacat attcgaccta aacctgcatt tactatttta tttaatatac gtcttgcact
     7861 tctttcactt acatttagat actctgctaa ctcttttgaa tcataaatct tactttttct
     7921 attctcattt atagcaataa tttttgcaac tgactcacaa ctcattccag tctttttaga
     7981 tatttcgatt accttttcat cagatagcat caaagaataa tttaattcat tttcggaagc
     8041 taaaggtcct cttatgtttt catcttcatc taccaaatat ataccaattt cttttgaatc
     8101 tatggagtgt cttaatgctt tatgtgcatt attttcagcc tgatacgcag ttactccagt
     8161 accaattcct atactaagag aaaagcccat gtcttttatc tctctcttta gattgaatag
     8221 cttatcataa ttatactcat tatcagctac ccctttatga acaaatacaa cataatcatt
     8281 tcttccaagt aaaaataagg aaccttgaat gcttcttaca tagtttacta caagcttatc
     8341 catatctgat ttttttatca tgtcagaata atagttatct atcttctctt tataatctat
     8401 taaactcaat atttcaacag caatttggga aaatcttgcc ttacttagag catattcact
     8461 ctttaccttg tcataacttt ctttgattaa ttgtatagta gcttgtaaac gaaatacagg
     8521 atagcctctt tcttttaatt cattatatac tgcaccaaaa ccagtcaaca ttatatccgt
     8581 cttattttct tcaaataatt ttatatgcca ttctacaagt tcagactctc ctacttccaa
     8641 agaaaatgga ttacaaaata cactcgttgc tttaatatca aattctttaa gtgcatcttc
     8701 cacaacatat ctatcaacta catctatact gaatttatct aattttatat ttgcactttt
     8761 tattgcccaa aatgccttca ttatactcgt cccacttctc ggtacaaatg aatgtggctt
     8821 acttatctca tacttagatt ttataaattc ataaactgca catcctgtaa atataatagc
     8881 atcacattcg tcttcacatt tagaaataac ttctattgta tctactactt tttctcttac
     8941 atatatttta gtctttaaat cactgtctat ctgttttaaa tcatccttta tcttaaaccc
     9001 agagtctgaa ggacctataa tacccactat cataaacttc gctcctaatc cataaatatt
     9061 atacttttta aaaattatat cctcactatt ttctacttct atttaattat tttgaactac
     9121 tccatcctac ctacatttag aatggaataa ttagcttatt tttgataaag taacttctca
     9181 aaatcagcac ttggaatagg cttacaataa taataacctt gaattaccac acaatctaaa
     9241 gtcactaaaa attcagcttg ttcttttgtc tctacacctt ctgccactac atccatatct
     9301 aaatcttttg ccatggctat agtgtgcttt aaaactattt tttctctgtc tgtgattcct
     9361 tcctcattaa agaaaccaca atctatcttt aatgtatcaa tattaatatt ttttaacata
     9421 tttaatgaag aatatgctga accaaagtca tccatattta aaacaatacc atccattcta
     9481 aggtcggcta ttatcttagt aaataatttt atatttttaa taaataaact ttctgtaagc
     9541 tctaattcta aaaaagattt tggtatacta tatttattta ttaaacttaa aacaatttct
     9601 ttaaaatgtg gattatgcaa atgtaatctt gatacattta cagagatagg aactggctct
     9661 tcacctaaat ctatccattt tctaatcaaa gaacatactt gttcccatac atacatatct
     9721 aatctgataa taaacccatt cttctcaaat agtgaaataa atttaattgg aggtattata
     9781 cctttttctg gatgaaccca tctaaccaat gcttctgcac caacaatttt atttgcatta
     9841 agattatact taggttgtaa atatacatgg aattgcccat tttcaagtgc gtattccatt
     9901 tccctttcca tctgtttttc ttcaagtgca tcatttctta tagtatcatc ataaaatata
     9961 tagttattga tactttgttt ctttactttt ttgtgagcat atcctgccat ttcacacgca
    10021 tttgtaattg gaatatgatt atccaaaatt ctataaaccc caaaacatgg tactaacata
    10081 tattcaaatt tttttctatt taagctattt ttaatcttat aaattacctt atgaatatct
    10141 ctatcagagt aactctcata aaaaatgcaa aatacatctg catacatacg acaacatata
    10201 ccattagaac caatgttttt ttgaatcaac tctgcaacat atattaaagc tttatctcct
    10261 cctctaactc cataaatgtc attaattacc ttgaacttat ctatatccat cattataatt
    10321 gcataacgct tgtcaggata tcttttaagc attaaactta tttgttcaca aaaactttca
    10381 taattgtata gtcctgttaa tgtatctttt tggtttttat gtagttgcat aagataatct
    10441 atatttttta tagcaactaa aatatgtgtt tgtttctcac ctaacttatt tttactacta
    10501 atctttaaca tataaattaa tgcccaatag tactttttat ctattcctaa acgtctatac
    10561 tcaaaattta gttctaaatc gtcttgcttg ctattattaa gactatcaag agattttaga
    10621 aggtggttat cattaaataa tattatatca tcaggatgaa ttaaatgttt ttttatgtat
    10681 gaaaaagttt tttcaagatt ttcctttata ggaaaactaa aaatattgct ctctgattga
    10741 taaattggag taattaaatt cgtatcaatg caaatagata aaattatatc atatgtatta
    10801 taagctaatt tttcataagc cttagaatac ccttttatca ttaaatcatc aaaataaacc
    10861 ttatcaacta actctttatg tttaaataaa atattattcg tcgaatcagc taagtcacta
    10921 attactttca tttcattatt tctaagtttg ttcttattat aatacctctt cttatcttgg
    10981 tacattctct catcagcaac tttaattagc ttatgtatat caatattatc gtcactccaa
    11041 atactgccca ctgctacact atatatcatc tcatccttaa attcttcact taaccaactc
    11101 actaattctt caaatagttg ttttgatata tcttcacata atacaacaaa ctcatctcct
    11161 ccaatacggt aaacattata agaatgaaag tattttttta ttattacaca tgctgacaca
    11221 atagcagaat cgccattagc atgtccaaag ttatcattaa tatcttttaa cccatttata
    11281 tctacaaata caactcctat ggatttaact tttccttttt gaaaattatc tatatactta
    11341 atatatttat ttctattata tgcaccagtc aagaagtcat agtagctcat ataatttaat
    11401 ttattttcta cttttctttt tttcatctca gttaataaaa aataagatag ggatgtcaaa
    11461 agagaggtat ctccaacagc tttatgtgga ttatcaactc caataaaccc tactacttta
    11521 tcatcttgtt ttagtgaaga tgtcattaaa ctttcaattt tttgaggttt taaaatttca
    11581 tattctattg gctgttcttc ttttatattt tctatatcat caattattac aaaacctttt
    11641 tgttcgaata ataaaaacca attctttaat tcattaatat caagatttgt tagtgcctgt
    11701 ttttgttcat caacaccttc attgcaccat tcatatgtat tagttacttt ttttctatct
    11761 tctgatactt caaaaacata tgctctatca gctttatgaa atttaccaat attggttaat
    11821 acaagattta cagcatcaga aaaattagat tctgttataa gatgtttaat acatccaact
    11881 aataactcct ttatctctaa tttttctgct attgacttac ttatatcttc cttttctgta
    11941 atatcaagag ctatttctaa tcgtgcatgt ttgccctccc atttaattaa cttgtccttt
    12001 aatataaaat gtctatttgt tcgagtattc gtattttccc atatgtaata ttcatcttct
    12061 tttaaaatag agtttgtaca aaattcgcag ggagaatctt tcccctgtaa tgctttataa
    12121 cattttaaat gggatatatc attaagtccc attgcctttt tcccaatttc attaatatat
    12181 aaaagttcat atgtatctaa agaagaaaca tatacaattt catccaactc atctattaaa
    12241 tttattacat ccatttatat atatctcctt ttattcattt tccagattga atttttaaag
    12301 cttttaaaat tttgctatat atttggcaat gatattatat ttctttatgt tttaaaatag
    12361 taccttaatg aggttctatt ttttagtaaa tagaattatt attgtgtcaa tcgtacaata
    12421 gagttttgta atacctaatt taatatattt ataattaatt atctcattat gtgtttataa
    12481 ttaattctca tataaatgac aaatctccac ctcaattaag tattccaatc atatatgaag
    12541 ataatacttc attcatttaa aaatataaaa ctattatata ttaaaatttc ttcaataata
    12601 aattatctag attgttactt tgatatatta tataatatca caaaggctga tataaaaaga
    12661 caaaaagggc aattttttta tatcaaaaaa aaatgttaaa ttgatattat ccatatataa
    12721 attgatatta tccatataga tgctatattt aacaattcta cttacttccc ttacacttat
    12781 tagcatatag ttattaagag tttgttgcaa aacaacactt agcacacttt aaaaagtgtg
    12841 gtctaataag taatcaacga agaagacata ataaagcata aaatatgata tacttttcca
    12901 cgccatcaaa actgaggatt ttattattaa tatatgctaa aatatgctta ataaattaaa
    12961 ttttaatggg agaatagtta tgaaaatatt tagtttcttg ttgtttgtga taggaatatg
    13021 tttatgtatt gctggattta taggacatta cccagtattt gttggtacaa cattgagttt
    13081 tcttgcaatt gcaatgatag ctgtaagaca agtcaaaact aagaaaaaaa tgaaataggc
    13141 aaatcctaac tttacaagtt aatattacac aagtaattag tctaaaattg gatacttttt
    13201 ctattacaga aatacatcat ccactttgta ttttcttaaa aaaataaaat agctatgtaa
    13261 attcaagatt agatagatta actctctatc catttcattg taaaatatac atagctattt
    13321 gtattcgatt taaaatactt agattttctt acataacaaa taatttctat ctgattatta
    13381 ctcatattca tttagtaacc actcagcaac taatcattta gtatctattc atccgccata
    13441 cggtaaccta ccccaacctc agtcaaaata tattctggct gagcaggatt cttttcaatt
    13501 ttccttctta tacttgccat aaaaactcta agcgactgat tttcactttc atagtaattt
    13561 ccccaaactt tatctattat aaacttatgg gttaagactc ttcctgaata cttagacatc
    13621 aactcaataa tcttatattc gattggagtt aaatgtattt cttcaccatt aattgacaca
    13681 atatgatttt cataatctat ctttaggttt ttaacttcaa aagactttgg agcatcatta
    13741 tttatattat cctgttgagc tttatttcta agagccacac gtactctagc caaaagctct
    13801 gttacactaa aaggtttagt taaataatcg tctgctcctc catctaaagc ttccacctta
    13861 tccctctcat gctctctcgc tgaaacaaca attattttat tagtctttgc acatgcccta
    13921 actcgtccta ttacctcaat cccatcaata tctggtagtc ctaaatccaa aattatcaaa
    13981 tctgggtcaa gagaatgtat aagtgatata gctgttcctc cattatctgt ctctacacac
    14041 ctataatttt gagtttcaag agatactttt ataaatttac aaattggctt atcatcttct
    14101 acaattagaa ctaatggctt tatttccact tgtctttccc ctcctcatca tacataggta
    14161 tgctaaatct aaatgtagct cctccagaag gattattaaa ggcgacaatt tctccaccat
    14221 gagcttctac tatagattta catatctcca atccaagacc aatacctctt ctttcaagat
    14281 ttctagactt attcttagta taaaacctat taaaaatatt cccgatatct tcatttggta
    14341 ttccttttcc attatctgca acttcaaata gtacttgatt tgactctttt tttacagtta
    14401 gttctatctt agaattttta ggtgtatgcc ttatagcatt atcaattaga tttactaaca
    14461 cttgttctat taataataca tccacatgaa cgattattat ctcatctgga atatttgttt
    14521 ttatgtctct actattggca aactttttga ctcttaatat agattcagct attatttcat
    14581 caacaacttc tggtcgtttt tcaatatcca attttccttc atctattctg gtcatactaa
    14641 gtatattctc aacagaatga gttagccaac tcgtatcttc atatatattt ttcaataatt
    14701 ctactctagt ttctttatct atcacatcat cattttcaag tattgttgaa gatgaaccta
    14761 atatacttgt aagtggagtt cttaaatcat gagaaattga cctaagcaag tttcctctta
    14821 atctctctct ctctgcctca agatttgctt ttttattttt ctcaaataaa ttttctctat
    14881 caattgccaa cgcaacctgt gttgaaactg cttctaatag tatcttttca ttttctgtaa
    14941 taatatctat cttaaaacac gcaacaccta taacaccaag aacatttgat tcttgacctt
    15001 ttatagggaa ataatatgca ctgctgtttt tacaatattt tgtcccaatt ccaactgggt
    15061 tacctgtcct aaaagcttct tttatagctt ctttttctaa tactgaatgg aatatattct
    15121 cacttccatc attgttacaa atataaatac ttggcttacc tagattatta ttctcatctg
    15181 caatagtcac tattacagtt ctatttaata tatttactaa attagaccca caaaattcaa
    15241 taatttgatt tttatttttt gctttaagca acgttttgtt attctcatag agcaatctta
    15301 tcattttttc tctcttatga gaaagtctca cttctctttt tatcctagaa gttaaagtac
    15361 ttgttataat tgctgccatt agcataatac aaaaggtcat aggataatct tttctataag
    15421 ctaaaaaagt atataatgga tgcgtaaaga aaaaattaaa tgatagcact cctattactg
    15481 aagaaagtat tccaaaaaaa tatccatagg ttatacttgt agcaaatagt acacctaata
    15541 tgtatactag tataatatta gattcattaa aacctacctt ttcaaaaact actgatattc
    15601 cagtagcaat tgtcataacc aatatagttt ttatgaagtc cttaataatt atctttttac
    15661 tgagatagcc atcttcatca taatcatact cttttaagtc ttcatgttgt agtcgttcct
    15721 ttttcattta ctacaattcc tccttattgt tactgtttaa attggctcta ttttatataa
    15781 tacaatttta tcataataaa ttcaatcaat acatttaatt aatgtactta aattactaaa
    15841 aggtgctgtc ttaaaatgaa aataaatctc tatttcaaaa cagcacctat atatttaaat
    15901 tactaattta gtttttacat acttaaatca atagttttat tcaatttcat aaattataat
    15961 acttcaatta taaaacattt ctatccaaca agcataaata taaagtgggc acatatacct
    16021 gcacataaac cacctatagt atgcgaaagt atcgctttac cagccaatgg tcttgcatct
    16081 aaagcatcca tcatacctat gtgtgtactt aaatatccac tccaacacat acccattgct
    16141 gtgaatactg ctatatcatt aggagttata gcgcctgatt ttataaactc tggtactaaa
    16201 gatattgctg ctccaacagc tcctaatgca gttacaggga atgctattgc ctctggtgat
    16261 gtgaatccaa ataatggttc tattatgaag ctaatcttat caccaatagc aggtaataat
    16321 tttatacctt catatgcagc tcctgtgtaa acagcttgac ctgtagcagg gtctgtagat
    16381 ggtccaaaag ttaataacat aactaatgta caaactacta aaactcctgg tattatagcc
    16441 atacccattt caacacctaa cttaccacct tcaagtatag catccaatgt tctttggaat
    16501 acattaccat ctcttatttc tctaaactct tcaagttttg cacctttatc atttatttgc
    16561 ttttcagtat catttttagg gtcataatta taatactttt tagtaaaagt caacataatt
    16621 ctaacactaa taatactacc tattattgcc gcaacattac ctattatagc tggtagtaca
    16681 tattctttac cttgagctat cataaaagtt gttacaataa gtcccatacc aaaggctgtt
    16741 cctaaattac ataaagcagg tacttgatac ttcttaaaat attgtgtaaa tgttttgtct
    16801 ttagcaaatg gtataatagc tggattatca gataaatatg ttgcaactac tcctgctata
    16861 ctagcaccag gtaagcccca tataggtctc ataagaccct taaatatctt attaactaaa
    16921 gatattactc caaactctga aagcaatgca ctaagtgcac ctgcaagaac tgccatagcc
    16981 attattaaaa atactgtgtc taataaaagc gcatgtgctg tactcattat tactttaaac
    17041 atcattcctg cacccatgat gcttcctaca tatccaaatc ctaccgccaa tagtacaata
    17101 aatacgaagg tttccatact tactgccttg actcttctct tatccgacag cttttccatc
    17161 ttgaaatctt ccccctttta attttaaatt aaggttccta acattggtac aaattttttg
    17221 gtacaaactg agttctaaaa cttacactcg taatgcaagt tgtcagtttt ttgaaataaa
    17281 aaaaaattac gtttatagaa taagaatatc ctctttaatt tggatactct tattctattt
    17341 tatgttatta tattatcatc cttttgttta aaagtcaatt ttttctattt ttgttgttta
    17401 attttattta tactcttttt atatttttca ttttctttta tctttgtaag tactccatat
    17461 aagttgactt ttctaaatac agtagttata aataccaatc ctaatgcaat tgaacttgca
    17521 cacgataatg tactcacccc atattctaca gcttttatat aattattggt caaaacagaa
    17581 tacatagtat tatatattcc ataacctggt actaatatta ttaaacagca aacacttaat
    17641 gtagttgctg gagtcatata aattcttgca cataactcac aatatatcgc aaaacaaact
    17701 gtagctataa aaaaacataa gttttctgtt actcctatat ttaaaagaaa tttatatgaa
    17761 aaccaagcta tacttccacc tattcctgct agtattaagt tttttccttt catattaaat
    17821 agcactccaa agcttaaggc agtaaaaaaa gctgctatta cttctataaa caaatgcact
    17881 tatatccccc ctaacttgaa gtacaatcca agtaccaaga acattcctac tgctatagat
    17941 acacctacaa aaaatacttc tgctaatttt gcaagaccag aaatcaactg tccatctaac
    18001 aaatctctta atgcattagt caaagcaagc ccaggaacta agagcattat tgctcctgct
    18061 attaaatggt ctaacgtctt tatgaatccg agctttaaaa ttaaaattga acaagaagct
    18121 ataataactg ctccaattgc atttacaaaa aagtcattaa gggaagattt acttaaatac
    18181 ataataacaa acttagtcag aatcccaaca gtaaatgcag ctataaattc ttccacattt
    18241 cctccaaata atatcgaaaa agttgctgct gcaatacctg caaaaaatat tgttattaaa
    18301 tctgagtaat agttatcttc acttatttca tctaaaattt tctcacataa ttcaatatct
    18361 aaattattta tacttattgc tcttgataat gagttaactt catgaaccaa gtttaggtct
    18421 actcttcttg agcttattct tttaattgct gaatgtaatt tcccatctac aaatacagat
    18481 gccattacag ctgttggagt tgcaaataca tctacgtggt ctattccaaa agattgacat
    18541 attctagcta tcgtttcttc aactctatag gtttctcctc cactttgcaa catagcttta
    18601 cctgcatttg aggaaaagtt taaaattcta tctatatcca caaaacatat ctcccattct
    18661 tttatagtat tattcatatt ttaatacaag atattattta tttaaactat tttaaataat
    18721 tcatatccaa tatttacatt ttatgaaaaa acttgttttt tatgcttgtc aaaatcaaat
    18781 tttaagcttg tttacaccat aaaatggctg aattaatatt tgacaaacac tttcaaccat
    18841 ttatataacc ttctaaattg tataactttt tactacaaat aaaattatat ctcttttaat
    18901 tctttctctt gagtatttag cttatagtaa cttcatattt atatatgtac aatataaaac
    18961 attattttta tgatacaaaa tataatttgt aaatgtatat atgtattaac tgtgtatata
    19021 taaattattc ttgaccttat cagttttttc atatatcttc atctatacca accatatatt
    19081 aataaatgat attacataga cttaagtata ttaaaaaact taaatttagt tatttaacaa
    19141 cataatactc taatttaatc tataaaaatc catacttaat ataatgcaac tcttatttta
    19201 acaccatcta ttacacaaaa caacatatta aaaagtaaaa tacaaaatat ccactataaa
    19261 ccttatttta atactgaata tatattataa tatatttaat gtagcttaaa tatagtttaa
    19321 acataaagaa aggaaacggt atatgctaag aaaagataaa gttctattat ctctacataa
    19381 attatgtgaa aatatgactt taaaagacct tcaatcagat aaaacaggat tcactacaat
    19441 agaaattgct aaccattcat ctcttgatag aagtaatacc agtaaagaat taaataacct
    19501 atacaatgaa aaaaaagttc tgaaaatatc tggtaagcca attttatttc tacatgctag
    19561 tattatagaa aatttaattg gccgtacttt gaacagtgat gaattttcta taaataattg
    19621 taatgagcta cttaaatcaa gtaaaaatga ctcagaagat attttttcaa cattacttgg
    19681 acatgattct agtcttaaac tacctataga aaaagctaaa tctgctatac tctatcctcc
    19741 aaaaggtctt aatacgttac taataggacc tactggtgtt ggaaaaagta catttgctga
    19801 aacaatgtac aaatatgctg tagaaagtaa aatcttttct tctgaaagca aattcgtagt
    19861 atttaactgt gctgaatatg cagaaaatgc taacttatta ttaagtaatt tatttggtta
    19921 tgtcaaaggt tcttttactg gtgctaataa ggataaattc ggcatagttg cacaggctga
    19981 tggaggcata ttattcttag atgaaataca cagattgcca ccagaaggtc aagagatgct
    20041 ttttcttttg atggacaaag gcatatacag acgattagga gaatctgaca ttatgcacaa
    20101 agctaatgtc tttattgtgt gtgccacaac agaagatata cattcttctc tgcttgggac
    20161 ttttttaaga agaatccctg ttacaatcaa cctacctgct ttacaggata ggtctttaaa
    20221 agaaagatta tctcttatta aatacttctt tattatagaa tcaaaataca ccaatgcaac
    20281 tataaaagtt cacaaagatg taattaaatc attactcctt tatgattgtt ttggtaatat
    20341 aggtcagctt aaatctgaca tacaacttat gagtgcaaga gccttcttaa attacaaaac
    20401 tggacttaag gataatatag aaatagattt atcccttgtt cctgattaca tctatattgg
    20461 tcttttaaaa ttgaatgaaa atcgtagtaa tctaaataaa ttatcagaac ttgattatat
    20521 gcttttttat gaatttaatg gtaataactt agaagttgaa tatacaaaag aactatctaa
    20581 atacaatata gatacatcaa acaatggatt taaatactct acagagattc cttatctgga
    20641 aaatgatata aggagtatgt ttataaatta catggaaaag tactctacaa aattactttc
    20701 gatatcttca gtaactcatt ctgattatac aagtaatgag atttttaaag taatcaatcc
    20761 aaagatttac tatgctgttg aacattcttt aaaagaggct gaaaaaatat cgagaaacaa
    20821 atacacaaaa caaacttata ttgctctttc aatgcatata agtgcactta tagaaaatat
    20881 aaatcaagac acatataatc ctaagtacaa tttatataat gataaaattt tcttagataa
    20941 agatttaaat atagctaaaa tagttttaag tataattgaa aatgacttac aaacatcttt
    21001 acctaaagat gaagtaaaat ttatatccat gtttctaaat agcatgtatc ttgaaccaaa
    21061 caatcaatct gaaagtatag gagtcataat acttgctcat ggaaattcaa cagcaagcag
    21121 tatgtgtgat gtagcaaatt cattgcttgg tacaaaccat tgtaatgcaa ttgacatgcc
    21181 tcttgatgta aaagtaagta ccatacttga taaaactata gagttggtaa aagaatgtga
    21241 cgaaggtaag ggtgtacttc ttttaacaga tatgggctct cttatagctt ttggagaact
    21301 tatttcagaa aaaacaaaaa tcaaaactcg ctcaattgaa atggtatcta ctccaatagt
    21361 tttagaagca gtaagaaaaa gtacactacc agaaatgact ttggatgaat tagctaattc
    21421 cttaagctct ttaaatccat atattggacg agcagcaatc gattcaacag aaataaacta
    21481 tcctgaaaag tcaagagagt atgtaataat aactacttgt ataacaggat atggttcagc
    21541 tatgaaagta gcagacttta taaattcttc acttaaaaat attagtaaat acaatattaa
    21601 tttagttcct cataatatag aaagttttaa aatgtataag aataaattta gtaatactaa
    21661 aattctggca gttgttggaa gtgtagattt taatgtagcc aacacccctt ttatacacat
    21721 agaggaacta ctaaatggat atggcctaga tatgctaaac aaaataatag aaggtacatt
    21781 caatgccaat agtattacaa atataaaaga aacaaatcta tcatacaaca gtttaataat
    21841 aaactttatg acccaaaact tagagctttt ggactctgtt aaattattca actatgtcaa
    21901 ttcttcctta tttacaataa tagaattaat agatgaaaac ttaaagagca aatttaagct
    21961 aggatatata ctacattgct cttttatgat agaaagatgt tttaaaaata caacttttcc
    22021 atataataac attactgaat taattagtaa aaatcatgaa ctatatagtt taatacgtga
    22081 agcctttaag ccaacagaag cttattttaa tattttgata ccagacgaag aaattgctta
    22141 tataatagac atgattaatg agtatctaaa taaataaaat ttatagcact tattttgtgc
    22201 atattaatta atatatgaaa tggaggacga gattttgaaa ggaaaaataa aacagttaac
    22261 aatacttgca ttaattttta tatttataac tcctgtattt gcatttgcag atactccacc
    22321 agtaccaaat tcttcaagag cagcactact tatagaccag gaaactaaaa gaatattatt
    22381 tgaaaaaaac attgatgaaa aaatgccatt agcgagttta agtaaaatga tgaccttttt
    22441 attggcaata gaagcagttg ataaaaatca agttaaagag actgatatgg tgaaaataga
    22501 caagtctaca gcttcagtag gaggttctac ttgtaaactt aaagatggtg atgaaatatc
    22561 tctaggagag cttatgcagg gtctaatgct agtatctgga aatgatgctg ctatagccat
    22621 agctaaacat attggtaaaa ctgaaaagaa ttttgtaaat atgatgaaca aaaaagcaga
    22681 agaaatagga atgattgata cgtattattt taatccaaat gggcttccta tatatactga
    22741 cccagaacat aaagaacctc ctattgaaaa tatgtcaact gctcacgata tagttacact
    22801 tggaaagtat atgtacgacc attatgaaaa tcaagttaca agaataacca ctatgcaagt
    22861 atataatgat actaaaaagg attttacaca ttataacaca aatcctctgc ttgtttcagt
    22921 acctggtgtt gatggaataa agacaggata tacagataat gctggatatt gccttgcatt
    22981 ttctatgatg gtacctaaag atgctaaaaa cgaaagaaat catagattga taggtgttgt
    23041 acttggtgat ggtaacaaga aaaatagaat ttcttcttcc gctactttat tgaaatatgg
    23101 taaagataat ttccattcta aaaagatagc acataagggt gatattatag agactccttg
    23161 tgttgatggt attgatgatt ttaagataac agttaaggtt gataaagatt tgtatggtgt
    23221 tgtttcagat aacgaaaata tcaatcctaa ggtagtgttt aaaaatatga attatcctat
    23281 ccataaggga gatattgttg gtgttgcaaa gtattataat gactctggta aatttgttgg
    23341 aagtgttgat gtaaaaagtg aaagtaatat aggatgtatt cctttgaagg ataagattaa
    23401 gattaaggtt gctaagataa ataaaaaact tgagattaaa aattctgttt gttttaaagc
    23461 atagtaaagc atattattaa aagggtatgt taaatttata aagactccac tttgtggagt
    23521 ctttttgttt tttacaaaaa attaaaatag tggcaacaat tgaggatgtg agggagagtt
    23581 gccactattt tgtatagggt attatattat gtttttaagc tagttttact atcttctatg
    23641 tttatataat aactcttgtg tgtgaattta attgataatt aatatgaaaa ttatgtgaag
    23701 ttttgattta tttgtaatat attctactac tattataatt tttctactgg atatactaat
    23761 catttgttta acattatctg agattgattt gcaaactttt atataactaa attagttata
    23821 taacttatag attccattaa ataccctaat aatcatatat gtacatacaa agtcatgctt
    23881 atgcaaaatc tgcagtttca actaacttat tatataaacc atctttatgt agaagttcag
    23941 catcatttcc agattctatt actctacctt tatctaatac atatatatta tcagcactct
    24001 taattgttga aagtctatga gctattataa taactatctt atcttgtatg tactctttca
    24061 agttgttcaa tatctctttt tgtgaaatat tatctagagc tgaagttgct tcgtccagaa
    24121 taagtacctt tttattactt actaaagctc tagctattga tatacgttgt ttttgacctc
    24181 ctgatatttt gacacctttt tctcctacta cttcgtccaa tcccttatct agatttttta
    24241 taaaatcatc taaacaagta acttctaata tctcatctaa ttctttatca cttattttat
    24301 tatttaatac tatattattt tttattgtgt catcaaatac aaattgatct tgagttacta
    24361 tatcaatatt atttcttagt gattttaaat taatatcctt tatattaaca ccatctatta
    24421 atatatcacc ttcatttata tcccaaagtc ggtatattag atttgttatt gttgatttgc
    24481 cacatccact tccaccaaca attcctgtta cttccccttt ctcaaattgt aaatttatat
    24541 catttaaaac aaggtttaaa tcctcaccat atctaaaaga tacattttta aaatctattt
    24601 tcttaatttc actgttatct attctatatc cattattatc aactttaata tctatctttt
    24661 catctattac tgaaaatact ctattgatag acacttttga ttgtgctatt ctagtattcg
    24721 cccttactat agctatacat ggtccaatta acattcctgt atattgttga aatgctatta
    24781 actcccccat cgtcatctta ttatttatta ttttatatcc cccaattcca tatattaata
    24841 ttgttatcaa tcctgttagt atttgagata ttgctatatt actactcata atgatgttca
    24901 aattcaaatc tttttttatc aaatctctct ctctctttaa atacttatta aagaaattct
    24961 tttttgaatt taatataaca atatccataa tattagatac gtactcttga atcaaatttg
    25021 ataaactgcc tgaatcactt cttatttcag aaattttctt agatataagt tttgtcattt
    25081 tattttgaga aaatgttaat atcaactgta aaaataaaac tactaaaaat aaatcaaact
    25141 gcaaattaat taagaaaatt aacgatatta tagctgtaaa cacatttaca ataatatcaa
    25201 atataatctc tgctccaaag ttttctatca taaatatatc actttccata atggataaaa
    25261 tattacctgt tttcttactt gaataatatc taccagaaag ttttgatata tggttaagca
    25321 actttacttt tagatttata gtaactttat tttttattga agtatataaa tatgataata
    25381 taacatcaaa tgtagctgat aatatcctta taaaaatata tatcaatatt aaatttataa
    25441 gcagaaaaat atttttattt cctattccat catctattat acatctaatt agttgaggta
    25501 ttacaatatt tacgccagca attataaaaa ccattataaa tgatactatc aacttagtct
    25561 tattcttata tactattata tttagtattc taaataaatt attattaata gtttttttca
    25621 tctatttttc catcttttat agttataatt ctatcagctg tctttgcaat attatcatta
    25681 tgagttatca taactagtgt ttgtttgtac tctttaacac accattcaag catattcata
    25741 acttcatttg tagtcttact atctaagttg ccagtaggtt catctgcaaa tattactgac
    25801 ggtctattgg ctaaagctct agctattgca actctttgtt gttgacctcc tgatagttca
    25861 ttcggatatt tctttatttg tttttcaatc cccaacttat taattaatct cataacatat
    25921 tctttatctt cttttttccc atcaagcaac acaggaagta ctatattatc atatacattt
    25981 ataactggta ttaaattgta actttgaaat atgaagccaa attcctgtct tcttatttca
    26041 gacagcttat catcatttaa tgtatataaa tctacatcat ctaagtacac attccctaaa
    26101 gttggcttgt ctaagcctgc aacacagtga agtagtgtac ttttaccaga accacttgca
    26161 cctacaatag ctgtaaattt atgtggctct atatctaaat caataccatc aagagctcta
    26221 acttcattct catattttcc atatattttt tttaaatctt ttaccttaag tattttcata
    26281 acgtatctcc attcccattt aaaaaacata tatttttaaa ttaaattttc gtaaataaga
    26341 tctactgcca tatctaaatt ctctaattga tataagtata cttctttgta gtcaatacta
    26401 atattaaact ctttatttaa aatagttaaa aaattatatg cattataatt tccaatacat
    26461 gtaaaattgt taaataaaga atattcactt acaaattcaa agtctatttt tattttagaa
    26521 aagtttctat ttatcacatt tattattttc cttgctatat ccaatcttat caaattagat
    26581 ttataacttg gagtgttcaa gttttctatt tttaaatctt ctttactttt gcttttttga
    26641 ataagtattt cttccaattc attaaactta atatatggat tgttttttaa tatatttgca
    26701 acaatgccag ttgccactgc acaagcctta ctatttttac caaaatgagt tactttatct
    26761 ttaaatttaa taaaattatc ttttgaattt gcatacattt gaatcttatt ttctggttcg
    26821 tatatatatt gattattttt atatatattt tcattaccta ctacaccaat aacactttca
    26881 aatatagtag gaatactttc tttatcttca tatttattat gttttgaaca aataataaat
    26941 ctaccttgac tatttaaaat attacaaacc ttttctaatt ctttttcata catactatta
    27001 atagatgcag ctgaaatgtt tattatctgt atatccgaca tagtcaactt gctcagtgta
    27061 ctaagaagat ttaaagaact tgtttttcca gaatcattaa atatattaat aggataaatt
    27121 tgggtatttt tacttatatt taatatggtt ttcgatacaa aagttccatg tccatttatg
    27181 tcatttattt ccttcccccc caaatctttc aattgaaaat ttgaatcata aacataattt
    27241 ccattaatat catcatcatt tacatctaat cctgtgtcta agactgctat tcttacttta
    27301 gtcaattaat aatcacctct aaaataaatt accttgaata taaaactaat ccttaccata
    27361 atataggttc caagatgttt ttcatttttt tacaatttgc atcaaacatt tttttgttat
    27421 tttttaaagt atatattttc gatggacaag ttgtacaaaa taaatttact ttacattctt
    27481 tacaactctc aatatttact ggttttaatt tatcaaatat attaaatata tccatttctc
    27541 tattaagaat cctctctaat atgttttcat taaattctaa tatattgaat atctttaaat
    27601 ccttatactc taagtttgga cacggatata catctccttt atgatctata cttatctgat
    27661 taacacctgc tttacacata tttgaacata aactactttc atccacataa tcatctttgc
    27721 aaatgtaatc aatatcaagt ttatcattaa gatatttgct atagttctca ttacctctac
    27781 caatattcat aaaatttctt attataggcg ttgtacctaa atcttcattt aatttattga
    27841 atttctctat gtctttctca ttattctttc ctaccaccat agacaaacct acgttctcga
    27901 atttttttag ttttataagt ctaatcacat ttaatacttt acagaatact cctttacctc
    27961 ttacttcact acaacttaat tcatcataac catctaaact aatttctatt gcatataaac
    28021 cattaattat atcatcaatc tgtttttctt taattaaagt tgcatttgta gacagaatta
    28081 ttttaccttt aaatttttct tttatataat ccaacagctc taagaaatca tttctcatca
    28141 atggctctcc acctgtaatg gttaatatcc ttggatttaa tttaactata ttatctatta
    28201 cttttcttac ttgagatgta tctaaataat cgttattatc tattttagaa tctacacagc
    28261 aataatcaca acttagatta catctattag ttggagaaaa tgaaattttg tttattctct
    28321 ctagtctaaa ttcacttaga atagaatcac ttagaagtcc tatatgctct aacttatcta
    28381 taactcgttc ataatatttt ttatcttcat tttcttggaa tacatcatac aaattaccaa
    28441 gttgtaatgc attatctact gaatacttta gaacttcata acattctatt ggaattttaa
    28501 cccatcttcc atttacttta ttactaagaa taacttgatt attatttatt attttttcac
    28561 atatacaatc taaaaaatat ttgtttgagc tattcattta ttttatttcc ttcctatata
    28621 tttaaattag aaatttcatt aattttcttt tataccactt actatactaa gtttatttat
    28681 cattttatta gaaaaataaa ctgcaatgac acaaacaaaa acagatatag atccaaatat
    28741 tagaatttca tatattggaa tttcaaattt aatagagtta tttataccat atacctgttg
    28801 attaagtttg ttagtttcag aaactatttt ataataattg aaactagata taattccagc
    28861 tacaatcata cttaataccc catatatcat attttctttt attaacattc tttttacatt
    28921 ttttttggac atccctattg ctctaaatgt acatatctca ctcaacctca caattatatt
    28981 tgatctaact ataaaaataa tgtttataga tgatattaat aaaactccaa atacacaaat
    29041 cattgctaat ttatatgagg atttattata atggtctcca aaatgattac gtacatatct
    29101 tccctctata tctgaaaatc cacttttttc tattatatta tttatttctt ttattacttt
    29161 tttatctgtt tcttttttta tcttcacaaa aactttatta taatcactta tccctgttaa
    29221 ttcttttaaa tccttttcat taaatataat ttgaattcca cctgattgag attctccatc
    29281 ttgctctgat atatagctat ttttcattat tccagcgatt cttacttttg tatttatata
    29341 tttatactct ccatctttat atacaggaat ttttatatct attaagtctc ctattttagg
    29401 tgattttata atctgagtat tatttgacaa tttaattcta gaataaaaat tatttaccaa
    29461 taaaacattt ttataatccc tgtctgtata actataaaca ttttcgcctt tatctatata
    29521 tttatctata ttatttaata gctctttatt gtaacctcga attaaaactg tagactcatt
    29581 attattattt atttcagttc tttttagctc atctaaataa tcttcttcta agtcagcatt
    29641 tcttagatat ccttttaaat aaatattggg ttcaacatct gttacatctt caatagcttt
    29701 tatttcattt aataaagaat tatcatattt agaaaatgta taatttgcat ttattggtga
    29761 atatcccaaa taaatatcaa aattgccata tgatttactt gttatattag ctgaatctga
    29821 ctcttttcca tatatgaatt ttgtagaagt tagtctgcct ataaataaaa ccccacatag
    29881 tgatatcgta attattatta ttattgatgt aaccttattc ctaaatatat ttttaaaagc
    29941 tatctcattt ataattccaa caactttttt atgtgatttt aacaacaaaa gcctaggaat
    30001 ataatttaac ttatctgtct tttttatagc atcaattata gataaatttc cagctttata
    30061 tattggtact acagaggcta atataactgc tataattgac atgatgatag attcaattat
    30121 agagtttgta tttatagtta attctgcact tttatcatat actatcatga gacctaagta
    30181 agaaaaaact atacctccaa gtaaaccaat tactatacct acagataaaa ctacaatact
    30241 ctgaataaaa aatacctgtc taatatttct cttgctagct ccaacagctc taagtaaacc
    30301 aatttgcttg gtcatatcta gtattattat gttaaacata ttgtatatta ctaatgttgc
    30361 acataataaa gggagtaagc tttgctttat tgaataagtt gttttttttg aaatattttt
    30421 catatacata gacatactta cttctgtatt tggataaaat tgattacttg ttaacttata
    30481 ttttctccct agattcacta ctttttgaga atctaaatct tttgtattca attttaatgt
    30541 acccatatga gttattaatt catatggaac aatatctttg ccattgtttg aatatgtaaa
    30601 agctcttacc ttatatttat ttaaattttc atagtattca tcagatttac ttaaaatacc
    30661 aactatttta aattgcttat ctttactata aatagaattt tcattatttt tatttatatg
    30721 ctcattaaca atctgaaaat caatagtttg atttaatttg acatctaatt ccattttgtc
    30781 taaaactttt tgttctatta ctaattcatt agaagttcta ggctctctac ctttaactaa
    30841 cttatatcta gtagaatcta aaaaatcttt attgtaagag cataaattta gagatgctcc
    30901 attatcttta cttataattt ctccaaattt aagtacatca ttgaattctt ttatatcttc
    30961 ttcaccatta agttctttta ctatatcact atttatatct tcataaaaag catgataatc
    31021 tcccgagttt ttgtatgcat gatttatatc tgattgtcct tgagattcat taataacact
    31081 taatgaaaaa actagcataa cagcaatccc tatactaagt attaatgata aagtttttcc
    31141 tttttgtctc tttaaatatg acaccgcaag tttaatattt atcataatca gaatcctcct
    31201 tattcatatt tacaagaaaa actctataac ctaaaatagt aggttataga gcactattca
    31261 ttaaatacta actaactcgt tgtgctagtt aaatttaatt attaaaaata ctagagttga
    31321 gaaaatcaca tttcatttgt aaaaaactac atctagtact ttcaacatat ttaatagact
    31381 gattagtatt ttttaataag ctactgttcc ttttggagtt gatcctgcac aatccaccat
    31441 acagtctatt tgtgaaatta ttccatgctt atcataatac catttacatg gatctgcttg
    31501 cacctcaaca tcttgaagtt ctattttttc ttcgattttt tttatatgta tcataataca
    31561 tatccccctt tacttttgtt ttatatttat tataactagt taaactagat tataaactac
    31621 atagtttttt tgttgtttcc acataaactc tttctaatcc acaataaatt ggtactgcac
    31681 tttggaattt acctgttacc tttttattga taagtttaca tctggttgac atacaataat
    31741 caagtctagt acatccatca cactcttcat tcttagatat ataaattttt ataaggttct
    31801 taattttttt atcatctaat ccatcatata tatttcctag taaaatatct ttatctccta
    31861 cacatactgt acatggatat acatctcctc tagtatctat tgcataacta tttatccctc
    31921 caaaacaatc tccttttttc ttatttataa attgtttatc taaaatagat atatctatat
    31981 caggataatc ctttttaata ttcataattt tactaatttg ttcatttaat attttaatat
    32041 gagaattatc ccaattttta tccataaaat ctgaaattgg tgcgatattt ttaaaccctt
    32101 gttttgctat atcatataca ctctcataaa gatacttaac agtatttgag ttatatgtca
    32161 ttcttgccct aacatatata cctttatcta taagtctttt tccattttcc attacttttt
    32221 cataagtacc tactccgtta gcatctattc tatttatatc atgtatattc ttttttccat
    32281 caatacttat cgatatgttt tttacattct ttgctataaa ctctattatt tcatcattta
    32341 taatagttcc atttgtagtt atatcaaata atatcttttt gtcactcaat ctatctacta
    32401 aatccctatg tatatattta atcaaatcaa aatttataaa tggttctcct ccatgtaaaa
    32461 caattgttgt ataatcacta ttactttcta attctttatt acaaataaaa tctattgttt
    32521 tatcagcagt ttcataactc atattttctt tttttttatc attaccttca taacaatatg
    32581 tacatctcaa attgcagtct tcagttacaa atatagtaaa tttcaattaa ctttttcatc
    32641 tccccataca gctgtattta aattgtgttt catcatacta caccatctat ccaattccaa
    32701 ttcattatcc ttaactgaat ctaatatcgc tgggcaatgc caacaaaaaa tatttacatc
    32761 acaatcttta catttttcaa agttatatgg ataaatactt tctaatttaa tcaaagactt
    32821 tagatctctt gttttatttt cttttataac ttttacaaat tcattgtcaa atatatttcc
    32881 cattttatag tcatccttta tcagtgaagg acaaggatat acatctccat cataatttat
    32941 aaatatttga gagtttcctg cattacatgc acaacatccc atttttttat tttttttatc
    33001 tttacacatt tttaattcat tatctaaaat atacggaatt gtaattggaa gaactgtatc
    33061 ttcaattaaa tagctttctt tattttctag gcctcttcct gttggaataa aatatctttc
    33121 aacaaattct gttccaagct ttttatttaa atctttaaat tgtttattta aatgagaatt
    33181 tttttgtcca aataccatag acagctttat attactgaat ccattgtctt ttagcagatt
    33241 aacagagctt attactttat taaatacacc ttttcctcgt gtttcactac aagtattttc
    33301 atctactcca tcaatactaa tatctatttt ttcaaaatta gttataataa aatcaacatt
    33361 atgttcatct attaaagttc catttgtgga taatattaat ttggtatcag gtaatttttg
    33421 ttttatatat ttaattattt caaaaaaatc ttctcttatc aatggttctc caccagttat
    33481 aattatatga ggagtattta aaactgatat tatatctacg atatttttta aatcatatgt
    33541 actaagtaaa tctttcttat tagaagaaga ttcaacacaa caatgtttac aaaataaatt
    33601 gcatctattt gtcaaacaaa aataaattgc aacaccattg cttaacatat caaagtttaa
    33661 attgttactt ttaaaaattt gatttttgtt cataatatct ataagattaa taatatattt
    33721 tttgtcactt tcatcatcaa aataatttac aaaatcataa atttttattt tattttcttg
    33781 tcctaaaaga aaatacttat atatttcttc agaca
//
LOCUS       DAMJVK010000039        32565 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.039, whole genome shotgun sequence.
ACCESSION   DAMJVK010000039 DAMJVK010000000
VERSION     DAMJVK010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 32565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32565
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.039"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(<1..591)
                     /locus_tag="PC414_001916"
     CDS             complement(<1..591)
                     /locus_tag="PC414_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDF2464312.1"
                     /translation="MLNETRKKQLTEVCQDLIRNASYSGQEENVVKAIEENFALLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEARIVDGKIYGRG
                     TSDMKGQVSAMMAAVSYFAEDTNKDFEGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKG"
     gene            complement(921..2135)
                     /gene="dpaL"
                     /locus_tag="PC414_001917"
     CDS             complement(921..2135)
                     /gene="dpaL"
                     /locus_tag="PC414_001917"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HDF2464313.1"
                     /translation="MREIKWKLNNLPKSEEKEKGIEFLKDEEIAKAKAFHESFPQYEK
                     TPLVKLDNLAKSLGVSGIYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLDTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAKEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNYSSAFVSAPDWVSAKGMRILGNP
                     LRGDEKVISGESGAVTTGALVSILESEDLKDLREALKLDENSKVLLISTEGDTDPDKY
                     RDIVWNGECQSK"
     gene            complement(2758..3297)
                     /locus_tag="PC414_001918"
                     /pseudo
     CDS             complement(2758..3297)
                     /locus_tag="PC414_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427977.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
     gene            complement(3374..5179)
                     /locus_tag="PC414_001919"
     CDS             complement(3374..5179)
                     /locus_tag="PC414_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2464314.1"
                     /translation="MLSKLKEFQQEMIKYTETVASVLDVDIEIVDDRLIRISGTGLYK
                     SKINESVVTEGFIYDNVIQTGQELVVLDICDNQLCIECSHYMKCLNKVIIAVPIKYNN
                     RTIGVIGAISTDKTKKVEISAKIDNYLKFVNHICDLISMKIEEHEASKNSSRKMDMMI
                     EIIENVEKGVIILDINSKISYINNIALKKLDIDKNIIENIVNIVSVESSSNGHELLEI
                     DIDNKIYNINAKIIPVYPYINQYDKIIIFDKTYINHKGHGKVNSGWGNSDIESIIGNS
                     EAMLKVKERTKKLAKSNSTVLITGESGTGKELIARAIHAEGSRWNKPFIAINCAAIPE
                     NLLESELFGYIKGAFSGASSGGKVGKFELANEGVIFLDEIGDLSMPLQAKLLRVLQER
                     KFARIGSNKLIDLDIRVIAATNKNLLKLVNEGKFRDDLYYRLNVIPINLPPLRERKDD
                     IEAIMMKFASKYSLELGIQLNKIEENVMNMLINYNWPGNIRELENAVEYMMNLVGDDG
                     IIYKDMLPLDILNYYNINGNIYKNKDVNIIFEDDIVGGIVENQERILSIKELELTYIN
                     KLLNKYGRDTKTKKKIAKDLGIGLATLYRKLEEEQ"
     gene            complement(5575..5952)
                     /locus_tag="PC414_001920"
     CDS             complement(5575..5952)
                     /locus_tag="PC414_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDF2464315.1"
                     /translation="MSKVVIATEKAPGAIGPYSQAIQVGNIVSTSGQLPIDMKTGELV
                     SDPKEATKASLDNVKAILEQAGTSMDKIFKTTVFVKDLKDFNDINEVYATYFKENPPA
                     RSCVQVAQLPKDAVLEIEAMAVL"
     gene            6857..7342
                     /locus_tag="PC414_001921"
     CDS             6857..7342
                     /locus_tag="PC414_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464316.1"
                     /translation="MSKSELTAETTEEMLEVLSGKDYDIACHLHELGKSLDCKIEPKT
                     GARSYKIVYSTKKPKRSLFTIECNEKKWRVKANLFHLNTYKDAVEECSKTIKDSITKT
                     RTCKKCNSKCIGGSSFELDGKSYLTCIGSGHYFANMEEMDWKNLEKLITKENNIMQES
                     V"
     gene            complement(7542..8081)
                     /locus_tag="PC414_001922"
     CDS             complement(7542..8081)
                     /locus_tag="PC414_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2464317.1"
                     /translation="MSKNVLIVSASPRKGGNSDLLCDQFIQGVLESKNQVEKIFLKDK
                     DINYCTGCGACYEKEANCSQTDDMGEILEKMIEADVIVLATPVYFYTMNAQMKTMIDR
                     TCSRYTEICDKDFYFIVTAADENVADMERTIEGFRGFTSCLNGSKEKGIVYGVGAWNV
                     GDIKGSKAMKQAYEMGKLA"
     gene            complement(8074..8487)
                     /locus_tag="PC414_001923"
     CDS             complement(8074..8487)
                     /locus_tag="PC414_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2464318.1"
                     /translation="MTISEVSKKYELSADTLRYYERIGLIPPVNRNKSGIRSFTEEDC
                     EWVNFIKCMRGAGLSIETLIEYVAMFQQGSSTIKARKELLIEQRNQLAKRIEEMQKTL
                     ERLNFKIDRYEEGIIEKEKVLKSSRNKKMEAVSYE"
     gene            complement(8523..9644)
                     /locus_tag="PC414_001924"
     CDS             complement(8523..9644)
                     /locus_tag="PC414_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDF2464319.1"
                     /translation="MEAKKLGFGLMRLPIKDENDVTTIDMEHLNKMVDTFLERGFTYF
                     DTAYVYHMGKSEIALRESLVKRHKRESFTIATKLPLMALKKKEEQETIFNEQLEKCGV
                     DYFDYYLLHNIGVSHYEVAKKFDSFKFIEEKKKEGKIKNIGFSFHDSAELLDKVLSEH
                     PEVDFVQLQINYLDWDNESIQSRKCYEVAEKHNKPVIVMEPVKGGTLAKIPEKAEKLL
                     NDYNPDMSISSWAIRFAASKDNVMMVLSGMSNMEQLLDNTGYMQNFKPFVQEEYNIID
                     EAVEIINESILVPCTACQYCVEGCPKNIAIPNYFALYNAEKQALNTGFSTQMVYYNNY
                     TKTYGKASDCIECKQCEESCPQHIKIIDALKNVAETFEK"
     gene            10232..11884
                     /locus_tag="PC414_001925"
     CDS             10232..11884
                     /locus_tag="PC414_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp21"
                     /protein_id="HDF2464320.1"
                     /translation="MNKKNLAILISGIMFFSSSSAIFADNTTKQERLIGKTRYETAVE
                     VSKLGWVQSKTAIIVNGNSIQSALCANPFAKLKNAPILLVNNNSIENSTKAELKRLGV
                     DNVYIVDSGNSISSKVENEIKSLNIKINKIVGNNIYEMSTNVLKEIDKIKKVENVAVV
                     NGLKGLSDAVSIASPAAIHNMPIILVSDENSNDSSINFLNNRSIKKSYIVGGNKILSD
                     NTVKKYPNRERISGSDRNNTNAKIIDKFYKDKKINNVFVAKDGINQENALVDSLSIGV
                     LAAKENSPIMISNGSLVNSQKEFIKNKDIDEITQVGGGYNQKAFEDILKLKNVNIRNK
                     TKKISLIQAKEILKQALPGSNILVCYYDEDDNEYDCKVIKDNILYEIEIDAYSGKIIE
                     IEKDDDLNDNNLGTQNISLDKAKSIALDAVPNGNLIYCKYDAEDNEYEAKVIKNKKTY
                     ELEISAFDGRIVDIDMDNDSDDDFDDNDKNNSNTNDNSSIISAEKAKSIMLDKVPGGK
                     IVKFKYDEDDKEYEGEIIKGKTEYEITINAITGKILEFEIDD"
     gene            12183..12932
                     /locus_tag="PC414_001926"
     CDS             12183..12932
                     /locus_tag="PC414_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005340256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2464321.1"
                     /translation="MNTILKVNNITKTYNKGQQPSLDKVSFNVSEGEFIGIMGASGSG
                     KTTLLNVLSTIDKPTSGDIFINNINLKSLNDTCAADFRKDNLGFIFQEYFLLDSLTIF
                     ENISVPLTLQKLNPDKIENMVKSLAERFGIASQLNKYPNELSGGQRQRASAVRAIVKH
                     PSILFADEPTGALDSNSATELLHSLQEANEELKTTILMVTHDAYVASFANRILIFKDG
                     HIIKELNKQQNNRHTFYELIIQEIAKLDTKR"
     gene            12945..14972
                     /locus_tag="PC414_001927"
     CDS             12945..14972
                     /locus_tag="PC414_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2464322.1"
                     /translation="MNIFYTAIKNLKKNFSFYSLYLLSVSFVITVFFAFTSFSVNKIM
                     LEKISTDGRVESMCNVISIFLMAFVIFYMAYSNRFFLRRRTKELGIYALLGYRKFTIL
                     SLLTFENVLICSGAFIIGLVLGALAHKGIVFGITALLNLSIDNSQIPFFNINAINKTI
                     IFIALVVLVLAVSNGKFFYKTPLVKLIRYEKSAEKKLKFRKIPAFMGFIMILSGYCLS
                     LDILRGAKSVWVSIGFYQMGLITMIIIVIGTVLFINSFLPYIMQNSKKNKRTFYTDMK
                     IIAIPNFIYRIRSNSKTLIMLTLLSATTLTVSSVMALSLYYPIASVSRIAPSEIEFRV
                     EKNKQIEDVKQIVSQYTSNTDDIIFTQTNIYKVKSSSKDLPFEYEIGTSKNNLSNSSS
                     SKEVISRESGFECISFSDYYTLLKAQNRTKLLKQLTPLRDNECILMKYQPNNDGYSEN
                     GTIYPLLVNGKETPVAVRETSLNNAISFANSVGTLIVNDSIYEQIAQNKLPMTRILSI
                     NGKSIKNNENLYSNISDYLDGSPYLQGYSHRINDLLSINSSTFLLIGFLVVLFFIAVG
                     SILYFNNISAISDSTSDYKILQKMGYTNIQIKKIIKKQVLPFFNIPFLLGIVDCIFAT
                     LVYKTALMQNLLGNSLSQYIPVIIAIFLTSVVYLIYYILTIKSCCKIVLKK"
     gene            15116..16699
                     /locus_tag="PC414_001928"
     CDS             15116..16699
                     /locus_tag="PC414_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2464323.1"
                     /translation="MTNFNLKILFLGNYILSLLFMGISTSTETYMNIQSIIPLIISTL
                     LLIDITYITSCNVRENNIISLFCGLLALDSWYLLLSFVTTPIGTIIFKILSTVIVYVS
                     IKFIFLFLFQGSGYNFRKITDILLLITCIGAIVGAFISNRTYACMYGIQFVVSIISFF
                     YIIFYHLKRVVFVIKSEKKPISISVFITIIGFLTYYFFTLRISNHIGNFGIYLTVLVF
                     FMSIHGIALKENDSFPISTVFNIKQRAIFLFISILVISLVVLFANGNFSIILIAINVL
                     FMLIFLCNIILEFNLRDEKNKVVKNSKYTFALEKLQQEEQLKTEFSNFLHDDILQNLL
                     SIKNMMSKSYRPDVQDIIYETLNNLNTCIRKQMQDYHPVILKNLTVKENYQNLIESVS
                     SSFPQKNICVSFKCSDTLFLVEPYDILIYRLIKELLTNVYKHSNGSHAWIALALEKNI
                     IKLNISDNGVANTSFKTPLDITKITFDTIKHKGIISIKEQVENLGGTISVSDNIPHGV
                     CVQITIPMKGDGSYKYFVS"
     gene            16671..17291
                     /locus_tag="PC414_001929"
     CDS             16671..17291
                     /locus_tag="PC414_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2464324.1"
                     /translation="MVLINILLVDDHMLFAKSLSIALEEYKEIEQFYSTQDIHNLSEM
                     IQDKKIDIVLMDINLRGLSECDGLKVAYNLLETNSTIKIIILTGYDLPVYKHEAKKIG
                     IYGFLNKNISPDSLIDSLLKVYQGVTCFPSENTEVIIEDLTTMEKRILQLICSGKKRK
                     IIADELYISERTLSNHLQHIYDKLDVSSSVEAITKAIKMGYISPVY"
     gene            complement(17480..18406)
                     /locus_tag="PC414_001930"
     CDS             complement(17480..18406)
                     /locus_tag="PC414_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HDF2464325.1"
                     /translation="MSNSFYQLNSAVLYFNEKRDVNRIRFNHYVMRKENIQRLKGTLP
                     RGQFMMTVRKAASDLDVSISTISRLVNEFIDLGILRLISRGVKGNCCSVYSYVCSESS
                     NAEIVTKNGDIFKNIEKYIDATINNNQKTMKYKHRNELFKNSNTNIYSRINQESIDKD
                     YDLIIHTGYETENETKKKELLKNNLNKKNLNKNTFENSDEDLTEDSTYKNPESIHEII
                     IRKLNSESGKNFKADLSITKALIDKRLKEGYSLEDFYKVIEAKVRRWRYTDMEIYIEP
                     TILFGYEFESYLNEESSLSACNYGDMCYEDMY"
     gene            19036..19956
                     /locus_tag="PC414_001931"
     CDS             19036..19956
                     /locus_tag="PC414_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2464326.1"
                     /translation="MTNAKSKKEYLNRICKVQDYIEEHLHEPLSLDELSNIAGFSKFH
                     FHRIFKAIEKETLAQYVNRLKLENATAFLIHRTDMTVTDIAHYFGFTDSAVFSRAFKN
                     YYKISPIKYRNNYSKNCKEPYKISQYNRSISKPKCKNTREVKGEIEILELDNMNTIYT
                     RYTGSYGNLTSTFPKLLERLFTYASEQNLLGLENTQILAIYHDNPQFTQEHQLRTSIC
                     MTIPDNIVVKENDNLGNMIIPSGKYLVGHFDISKSEYSDAWDFMYNEWLSNGNYKLRA
                     SFPFEVYLNNPHNHPQNRHLVDIYLPIEPF"
     gene            20042..22462
                     /locus_tag="PC414_001932"
     CDS             20042..22462
                     /locus_tag="PC414_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDF2464327.1"
                     /translation="MKEIKINGAKIHNLKNIDVSIPKDKLVVATGVSGSGKSSLMFDI
                     VFEEGRKQYLQSLGILAGIDSEDKFDNIQGLAPTIAVQQNIIRQSNPRSTVGTRTNIL
                     NMLTLLYSVEGQIMCTMCDTPVSYNLICNNCGYEEERLRPSYFSYNSSDGMCMKCSGH
                     GAYFEINIEKLVLDKHDTLKQVLDRAKITPGYLRVFSKKFNDYLDIQFLQLPEEVRNE
                     VLYGHYENGKKSSSLSKVFHNRYKRGEDLNGVYTMTACSDCNGFKIGEEARRVLLNGK
                     HIGELGKMTILEIDDFLKLLINQGNLNTFGTNLVNDILSKTQHLIESRLGHLSLYREM
                     STLSGGEMQRLFLNSHLDSEMDSLIYVLDEPTAGLHESEKIDILKSIKKLKDLGNTVI
                     VVEHDKNTIKMAEHIIDIGPKAGVEGGQLVYQGDLEGLLQSDKSLTGQYLSGKYPMPN
                     RTSNKNITHTDKIPCITIQNANTNNLKNVTVSLPLGAMVGISGKSGSGKSSLISDTLL
                     PLLRSQFNNHTINNQINSSESEIGEEDDYAVVETIADRLIGTKHISGYSEISQSPIGK
                     NMNSTPASYIGIWDKIRTLFAGQPESLERGFTAGHFSFNSKGACPKCSGSGYEKVWLG
                     NNLSIDHICSECQGKRFNDESLSIKYKNKNIHDILNMSVSESVHFFKDIPNIVSHLNV
                     LEQIGMGYIKLGQPTPTLSGGESQRIKLAKEIGKKRNGNILYVLDEPTTGLSLYDTSK
                     LIQLLDELVENGNSVIVVEHDIDVLNVCDWIVELGPEGGDKGGYIIAEGSPKTLKENP
                     KSITGRYL"
     gene            22462..23484
                     /locus_tag="PC414_001933"
     CDS             22462..23484
                     /locus_tag="PC414_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HDF2464328.1"
                     /translation="MNKNNCLDLIFEKVNPSSVGMSSEKLSQLEPLIISQYSNINGII
                     IARKGKVVFEKYYNGYNADDTFHVASVTKSIISALIGIAIDKGYIKDVDQKVVDFFPE
                     YTYSPSEICKKAVTIRHLLTMTAPYPFKNMTESLERLCKQKDWIKYTLNQLGQKGEIG
                     TFKYSSAGTHLLSAIITKTTGKSAREFANEYLFKPIGMKVIPDYDMKDFGFEDFFGKN
                     LKGWAKDRQGYSIGGWGLTLSARDMLRFGFLYLNDGIWNNKRIISKSWIEESVKMNSN
                     EYGYLWWLCKKDGVLWYSAMGAGGNAICCIPQYDLVIAIPSKIVFKPRDRWEFIEKYI
                     LTSIID"
     gene            23599..24345
                     /locus_tag="PC414_001934"
     CDS             23599..24345
                     /locus_tag="PC414_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2464329.1"
                     /translation="MDTYTTSEVADLIGVHPNTVRLYEELNLIPKPKRNTNGYRIFND
                     FHIEQFRFARTALKIEVVQRGLRKDALEIVRTSANRDFDKAISLVNQYLQQLEIEKSN
                     AEEALTIVAQILNGNTPETNKLFLTRKETAEHLNVTIDTLRNWEMNGLLKVKRKQNGY
                     RVYTDEDIKRLKIISALRCANYSLSAILRMLNALSSNPNTNIKTVLDTPKDDDEIISV
                     CDKLITSLKEAEHNAKTMKSHLQIMKKQFS"
     gene            24431..25681
                     /gene="cme"
                     /locus_tag="PC414_001935"
     CDS             24431..25681
                     /gene="cme"
                     /locus_tag="PC414_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Cme"
                     /protein_id="HDF2464330.1"
                     /translation="MENKNTNSTWKKKVILFLASQYISLFGSSLVQMAMIWYVTLQTS
                     SGVWVTVLTVCSFIPQMIISFFAGVWADYYNRKVLIIVSDSTIAISTLVLILFMMSSN
                     GTTSELIAISVISVIRSLGSGVQTPAVNAMIPQLVPEEHLMRFNGINSSLQSIVQFIV
                     PIVAAAILGFGNKINQVLLIDVFTAIIAIVLLIFIKIPKHDIPKQTEKVSFFADMVSG
                     IKYCFSDKFIRKVLFTYGAFMLLCVPSGFLIGLLIERTFGKGYIYLSIIEMIGFLGMV
                     LGGLIIGVFGGFKNRNKTFFLAILSYAIFSIILGLVTQAWQFFIFMFFTNFSIPIIQA
                     SAMTMLQEHVAPQMLGRVLSLPIIIYTGFIPLGMMIFGPMADVISINNLIIIAGIILL
                     FFALAIPYSKQFYKQGISKSNESE"
     gene            complement(26198..27211)
                     /locus_tag="PC414_001936"
     CDS             complement(26198..27211)
                     /locus_tag="PC414_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2464331.1"
                     /translation="MIGLRYQLKSIKKDKMCIISFFLPVVMAILINFMGTIDISSLGE
                     IRFGIVKDTLPVQTEQWLKKYGMVSVYDTKMHLIREIKDIKTDTIGVVRDGKGIKTVL
                     YGNEINAIKNVGKTLPEMFRLKGDLKDVEVTILPKDSILKEFQNVFIAMILITAMFMG
                     CTFNAMNIISEKEDGISFINEVLPQSRSQYIIQKVFLGLLCGCLSSMITVFICLDISI
                     LDMIMMLLLVVFSAFISSLIGLFIGKLSEGLMVGIVYIKIVMILFIGTPLLIYLLGVN
                     TSGIGNLYYIMPSVATFNGIMEIINGNVIIIKDIVILAVHCVVWFMIYIILDKKRKLF
                     FSR"
     gene            complement(27214..28242)
                     /locus_tag="PC414_001937"
     CDS             complement(27214..28242)
                     /locus_tag="PC414_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2464332.1"
                     /translation="MYSKFFTLVKKDFWMMIYGKFFVLTLGSLILYSCYINFGYVKFM
                     DEDSYHNNVYLYNPENISMSASPLINQVSSIGELKAKLDEDTSAVCIDMSSSKPKVIL
                     HESIKGVDDYRADYALSLLSSEKDHNSKISDNNAIIQINDKDTSEIKQRKDMTCEILF
                     FEIVAIGFLGIASLLFKEKQMGVMRITGILPVKRSLFILSKIFAFLICDLCFTVLLVV
                     FNIGFFEGIKILPQVLVQASILSIIMSLIGIGCSMLLRDFKQFSLIYLVVAVFITTPI
                     FLSANTSFEIDWIKYHPFYHVYSQLKNAFFMNFSKELVYFVVCSLTIIVLFLLVKYIF
                     NKELVREG"
     gene            complement(28242..29087)
                     /locus_tag="PC414_001938"
     CDS             complement(28242..29087)
                     /locus_tag="PC414_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2464333.1"
                     /translation="MIKVGELKFSYGKDKQILHGLNFEVKEGEIFGFLGPNGSGKSTT
                     QKILNGVLKGYNGHVSLFGKEVKAYTESLYQKIGVLFEFPYLYTNLSAIDNLEYFSSF
                     YPKNQRRDIYELLDLLEFKKEFINKPVSSYSKGMKQRISMARALVSNPKLLFLDEPTS
                     GLDPSGAVLFRKIIEEERKKGTTIFLTTHNMLDADLMCNNVAFIADGKIMVIDKPKNL
                     KMKNSNNKVEVEFVYNGNRDIEIVDIQELESGITFKYDEILSVHSKEPTLEEVFIKYT
                     GRMLV"
     gene            complement(29507..30637)
                     /locus_tag="PC414_001939"
     CDS             complement(29507..30637)
                     /locus_tag="PC414_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2464334.1"
                     /translation="MNSLRKIIEKLKNVDISNWIKQNFGLYSVRKKVFLLSKLSGALI
                     ILFYLVVEEFSINSKLEFWIWFIILVIFIMSIDFLLGKFISEPITSINKSAKSMSQLD
                     FSNPCTVNTNDEFGELSRSLNTMSTNLQQALSDLESANIQLEKDVNKERMLLEQRKEL
                     VDTISHEMKTPLGIIRAYTEGLMDEVDEEKKKNYMNVIIEETDRMNNMIVSLLDLSAL
                     EAGVSKLNPERFDFIEFVETVAGRLLIDIPDANFYFTYDLPEYEVFVVADKMRMEQVV
                     ENLVINAKKHVVYNGNLDLSITCENGLLLFEIYNDGRFIEQDEISKIWSKFYRSVQSQ
                     RTGGSGLGLAIVSQILTMQGLEYGVNNRDRGVEFYFMIPIDE"
     gene            complement(30634..31305)
                     /locus_tag="PC414_001940"
     CDS             complement(30634..31305)
                     /locus_tag="PC414_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2464335.1"
                     /translation="MAKILIVEDEKRMQSIIVEYMQKGGYTCITADDGVEALTILKSN
                     NIDLMILDIMMPYLDGFSVCRVSREMTNIPIIILTAKGEEEDKLKGYEYGADDYITKP
                     FSPKVLLAKVNALLRRYTTDIPKNSLSLGKIFIMVASRQVYVEDKLIDLTYKEFELLR
                     LFMENPNQVFSREKLLNCIWGYDFEGNTRTVDTHIKTLRKKLGSEGHHIVTLIRSGYK
                     FEVKE"
     gene            complement(31519..32169)
                     /locus_tag="PC414_001941"
     CDS             complement(31519..32169)
                     /locus_tag="PC414_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDF2464336.1"
                     /translation="MDFISSILQGLLSILFNFTGDFGISIVLITLIVKLVLMPLSLKQ
                     RFSVKKQQELAEKMEHIKEKYKNDTKELEKQIQVSSVESMKSMLGCLIVLVQMPVVYA
                     LYHVCLDMTKEFTTIIIPWIANLGTFDNLFILPCIYTLIMLASNLVNYIPYLKINSQV
                     KFNKQMAVVTTITSLILTVRTPVAIGLYFITSAIYSLIEDICFRIYFNRKNRIVTK"
BASE COUNT        11407 a         5115 c         4045 g        11998 t
ORIGIN      
        1 acctttttct ggattagcag agtgagctgg tttaccaaaa gtttctacta ctatttctcc
       61 tctacctctt tgacctatct ttaggtttaa ttctgaagat tcacctatta caacataatc
      121 aggttgaaca gccttactta tctctctagc tgaaacacct tcaaatattt cttcatgaac
      181 aacacctgct acatacaatt caccttcaaa gtctttgtta gtatcttctg caaagtatga
      241 aacagcagcc atcatagcac ttacttgccc cttcatatca gaagttcctc taccatatat
      301 tttcccatct actattctcg cttcaaaagg aggaacactc catttacttg catctggaac
      361 tggaactgta tctatatgcc cgtcaaataa tattttttta cctggtttat ttccttttat
      421 acatcctact atatttccat atctatctct tgaccaacta tcaaaaccta gtaaagcaaa
      481 attttcttct atagctttta ctacgttttc ttcttgacct gaataactag catttcttat
      541 taagtcttgg catacttcag ttaattgttt ttttcttgtt tcatttaaca ttttgatatt
      601 cccccatata atttttaatt tgttgacgtt ttattttgat atttcatttt tttaatttat
      661 tagcatttca cttaatctat tgatttttta tttctttata ttttatttat taaattttta
      721 tttagacttt attttaattt tatttggttt ttattttgaa tttttcattt gctttacttt
      781 tttatttgct ttactttttc atttatttag atttttcatt tgttttagtt tttattttat
      841 ttaaagtttt cacttattta agtttttatt ttccttaaat ttttcattta tttgttaaga
      901 ttttcttttc cttaagtctt ttatttactt tggcattcac cattccaaac tatatctcta
      961 tatttatcag ggtcagtatc tccttctgta cttattaata atactttaga gttctcatct
     1021 agtttcaaag cttctcttaa gtcttttaaa tcttcacttt caaggattga aactaatgct
     1081 ccagttgtaa ctgctccaga ttctccagag attactttct catctcctct tagagggttt
     1141 cctaatattc tcataccttt agcagatacc cagtctggtg ctgaaacgaa tgctgatgaa
     1201 taatttttta acacttcaaa acctatagtg tttggttctc cacaagcaag ccctgccatt
     1261 atagttggca tatctcctcc aacaaatctt ggttttccat ctccagcttc tgctgattta
     1321 tagtaacagt cagcctcatc agcctctact actactgtta ttgggcattc atcatatatt
     1381 gaagcaacat atccttgaac tgcaccagca agtgaaccaa ctcctgcttg aacaaatacg
     1441 tgtgttggtc tatctactcc atattctttt aattgttcta tagcttcttt agccatagtt
     1501 ccatatcctt gcattatcca tgctggtatt tcttcatatc cgtcccatgc tgtatcttga
     1561 accattactc catggtcatc agcttctgca taatcattag ctaatcttac tgcatcatca
     1621 tagtttaagt ctgttatact agcatctgca ccttctcctt tgattttatt taatctaaat
     1681 tcagaagaac cttttggcat taatacaaca gatttttgac ctaatttatt agctgtccaa
     1741 gcaactcctc taccatgatt tccatctgta gctgtaaaga aagttatttc tcctaatttt
     1801 tcttttattt cttttgaagt taatttatca taccctaact cagatatatc tgtatctaat
     1861 ctttgagcta agtatctacc catagagtaa gaaccaccta aaactttaaa tgcgtttaaa
     1921 ccaaatctat aagattcgtc tttaacatat attccgctta cgcctagtga ttttgctaag
     1981 ttatctaatt taactaaagg agttttttca tattgtggaa agctttcatg aaaagcttta
     2041 gcctttgcaa tctcttcatc ttttaaaaat tcaattcctt tttctttttc ctctgattta
     2101 ggtaagttat ttaatttcca ttttatttct ctcatttatt ttatattccc ctttcaaatt
     2161 tttcggtgtc attttaattt tttatctttg tgccttatag caaagcaatt accgtgccaa
     2221 tattatttat tctaaaaaca atttttttat gttggaatta catactttta taaaatttct
     2281 ttaaagttta taaattttga gtgtgaaaaa taagatttat aaaagaaaaa attctcatat
     2341 tgggaaattt ttatcaatac gagaattatc aattcgagaa atcgtttatt tatcttaatt
     2401 cttcctttat tatgctctta ttttttatat gtctattgtt ttgtcagata tattactact
     2461 tttattttct ataaatatat ggctataacc ttcttacgca taaactctat aaatttatat
     2521 actttatatt ctttattacc caaatattct ttattaccca aagcctccaa aatggcttaa
     2581 atcttttcat agcttcttac agaagtttac atgggcgtcc ccccaataag caaaagtgca
     2641 atatgtgtac cattgtgccc tacttgcgac tattgagctg tcgaaattcg caacttcgca
     2701 tttatctgat gaaatgcact atacaaaata ttcataacat ccacaacaga gttaaagtta
     2761 cctattcata ttcactgtaa aaatttcttc cacaggaaat gtaattttaa ccaatcctat
     2821 tgagacaaac tcaccactta tttttttgaa ctcacgcagt aatacatcac ttgcccgaaa
     2881 aaaagcctca ctttcatcat ctccagtaag tgctattgtg caatggggta cccaacgatt
     2941 ggggtggtac cattcccaac catttgaatc aaaatcttgt agtacctcat ataattctct
     3001 ctgaaactgc tacatactgc tattcattat aggtgataca aacaccgttt tagtatcatt
     3061 aaacatgcct actgaaccaa tgtaagcagg tattacctta tggttttgtg caaacccctt
     3121 tagcttgtct atacaacaag cctcatccac atcattgaaa caagccaaag taagatgagg
     3181 tcttgttttc cattctaaat acttcgtact aattttttca tcagcgattt ttttagataa
     3241 atcaaaaagt tgcttttccg tttttttatc ataatacagc tctatcgcat attgcataat
     3301 aacacctcca ccataaagta aaaataccta tctataaaaa cacacaaata cctatcattt
     3361 taatattata ttactactgt tcctcttcca attttctata tagtgtagcc aaacctatgc
     3421 ctaagtcttt tgctatcttc ttcttcgtct ttgtatctct tccatactta ttcaacaact
     3481 tgtttatgta agtcaactct aattctttaa ttgaaagtat cctttcttga ttctctacta
     3541 tccctccaac tatatcatct tcaaatatta tatttacatc cttattttta taaatatttc
     3601 cattaatatt ataatagttt aaaatatcta atggaagcat atctttatat attattccat
     3661 catctccaac caaattcatc atatattcaa ctgcattttc cagttccctt atatttcccg
     3721 gccaattata gttaatcaac atattcatta cattttcttc tattttattt aactgaatac
     3781 ctaattctaa agaatattta gaagcaaact tcatcattat tgcctcaata tcatcctttc
     3841 tttccctaag aggtggcaaa tttataggaa ttacattcaa tctatagtat aaatcatccc
     3901 taaacttacc ttcatttact aatttcaata agtttttatt tgtagcagca atcactctta
     3961 tatctaaatc tataagttta tttgacccaa ttctcgcaaa ttttctctct tgtaaaactc
     4021 taagtaactt tgcttgtaaa ggcattgaca aatctcctat ttcatcaaga aatataacac
     4081 cttcatttgc aagttcaaat ttacctactt tacctccact actagctcca ctaaatgcac
     4141 cttttatata gccaaatagc tcactttcaa ggagattttc tggaatagca gcacagttta
     4201 tcgcaataaa tggcttgttc catctgctac cttcagcatg tatagcccta gcaattagct
     4261 ctttacccgt accactttcc ccagttataa gtacagttga atttgacttt gctaatttct
     4321 tagttctctc ttttactttt aacattgctt ctgaatttcc aattatagac tctatatcac
     4381 tatttcccca gccactattc acttttccat gtcctttatg attgatatat gtcttgtcaa
     4441 atattattat tttgtcgtat tgatttatat atggatatac cggaattatt ttggcattta
     4501 tattataaat tttgttatca atatcaattt ctagtaattc atgtccattt gaggaagatt
     4561 caacagatac tatatttact atattttcta ttatattttt atctatatct aattttttta
     4621 gagctatatt gtttatataa gatattttgc tatttatgtc tagtattata acgccttttt
     4681 caacattttc aatgatttct atcatcatat ccatttttct actgctattt tttgacgctt
     4741 catgttcttc tattttcata gaaattaaat cgcatatatg attgacaaat tttaaataat
     4801 tatctatttt tgcacttatt tctacttttt tagttttatc tgtactaata gcaccaatta
     4861 ctcctatagt tcgattatta tactttattg gaactgctat tattacttta tttaggcatt
     4921 tcatgtaatg ggaacattct atacaaagct gattatcaca tatatccaat acgactaatt
     4981 cctgacctgt ctgtataaca ttgtcataaa taaagccctc agtaacaacg ctttcattta
     5041 tctttgattt atacagacct gtaccactaa ttcttattaa tctatcgtca actatttcaa
     5101 tatctacatc taacacacta gctactgttt cagtgtattt tatcatttct tgttgaaatt
     5161 cttttaattt acttaacata gtttccccca ttagaattta atttaccaaa ttatcagttt
     5221 agctatattt taacatatag tcactcttta aaatatatat aaaacattac ttatttatat
     5281 atttgcgtta aaacagacaa tattaattta taaaaagttt gttttatata gtaaccttaa
     5341 ttataatatg cagacaataa aataaatcac aatacaataa taaaaaaaag ctttcctcat
     5401 ataacaaata tatttttgac ataaaaatat acattatatg agaaaagctc aatagttaaa
     5461 tatttagata gatttttata tagacttttt taatataaac ttttttaatt gtagactttt
     5521 tttattagtt tttaattttt attagttttg ttattggttt tattgttata atacttataa
     5581 tactgccatt gcttcaattt ctaaaactgc atcttttggt aattgagcaa cttgtacaca
     5641 tgaccttgct ggtggatttt ctttaaaata tgtagcatat acttcattaa tatcattaaa
     5701 atcctttaaa tcttttacga atactgtagt cttaaatatt ttatccatac ttgtaccagc
     5761 ttgttctaat attgctttta cattatctaa agaagctttt gttgcttctt ttgggtcaga
     5821 tactaactcg ccagtcttca tatctattgg aagttgacct gatgtagaaa ctatgtttcc
     5881 aacttgaatt gcttgagaat atggtccaat tgctcctgga gctttctctg tagctattac
     5941 tacttttgac atgacttcac ctcctattat taagttatat ttacaggaaa ataaaatttc
     6001 ctaaacacca atattaatac gaacctttag attaaacttc aaatctaaaa cttcaaataa
     6061 tcaatactta gatgattatg tttatttgtg tcttgttaac ttttattaaa gcaattaacg
     6121 tgccaaactt tatttatata taaaattttg tgaagtcatt caaaatagtt acttctgaat
     6181 tttttcgtaa aaaaacgctc taaaattact tttttatgat gtcaattttt ttctcatatc
     6241 tagaaattta ccaacatgag aattcttgtt ttgagaattt acagattaaa gttatttttt
     6301 aaccttaaat tctgaacctc ccatgactaa agtcacaggg ttcctgcttc atagaatttt
     6361 gcataatcaa gttattacat atgaaagaac tatagttgac aatcccacta ttataaataa
     6421 taaaaccttt acccatacct tatttaccct agttatcata gagcctattg aaaatataac
     6481 agctaaatat aataataacc aaaaaattat caatatctac cactgttgct gaaaatccaa
     6541 aaaactacct tttgatagta ttacttaaca atctaattaa tctcataata aatttacaac
     6601 ttataaataa attgttatat tggccgtatc tcaacaactt tctcctttcc tagttttaaa
     6661 tcttttatag aattgtacca cgtctaagta aattttcata ctattaagtc ttatttctaa
     6721 taaatcatta gctctcattc tatgcccaat aatgtaagat tgcagtaatt ttattgattt
     6781 taatagtttt atttaatata cttatattaa ttattttcat atgaaattat tatttatata
     6841 gggggatttt tttaatatga gcaaaagtga attaacagca gaaacaacag aagaaatgtt
     6901 agaagtacta agtggtaaag attatgatat tgcatgtcat ttacatgaac ttggtaaatc
     6961 attagattgt aaaattgaac caaaaacagg tgctcgttct tacaaaatag tatattcaac
     7021 taagaaacca aaacgtagct tatttactat tgaatgtaat gaaaaaaaat ggagagttaa
     7081 agcaaatctt tttcatctaa atacatataa agatgctgtg gaggaatgct ctaaaactat
     7141 taaagatagt ataactaaaa ctcgtacttg taagaaatgt aattcaaagt gtattggagg
     7201 ttcttcgttt gaattagatg gaaagtctta cctgacttgc ataggaagtg gtcattattt
     7261 tgcaaatatg gaagaaatgg attggaaaaa cctagaaaaa ttaattacta aagaaaataa
     7321 tattatgcag gaatctgtat agttttttaa gggtaattat taaaatatat gagtaacaac
     7381 agatacttaa ataggcataa aaaaataaag ggtataataa ggatatagaa taaaaataaa
     7441 tgatatagca caaagaggct gtctcaaaat acagataagc ctctttttat tgttgaaatg
     7501 ataagaatat attcaatttt atacaatttt atacaaacat actacgccaa cttacccatt
     7561 tcatatgcct gtttcattgc cttacttccc ttaatatcac ctacattcca agcacctaca
     7621 ccatatacta tacctttttc cttagaacca tttagacatg atgtaaatcc tctaaatcct
     7681 tctattgttc tttccatatc agccacattt tcatctgctg ctgtaacgat aaaatagaaa
     7741 tctttatcac aaatttcagt atatctagag caagttctat caatcatagt tttcatttgc
     7801 gcattcatag tataaaaata aactggagtc gctaaaacaa tcacatctgc ctcaatcatt
     7861 ttttcaagaa tttctcccat gtcatctgtc tgtgaacaat ttgcttcttt ttcataacaa
     7921 gcaccacatc cagtacaata gttaatatct ttatctttta aaaatatctt ctccacttga
     7981 tttttagatt ctaatacccc ttgtataaac tggtcacaca ataaatcaga atttccacct
     8041 tttctaggac tagctgatac tattaataca tttttactca taagatactg cctccatttt
     8101 cttatttcta ctgctcttta atactttttc tttctcaata ataccttctt catatctatc
     8161 aatcttaaaa tttaatcgtt ccaaagtttt ttgcatttct tctattcttt ttgctagttg
     8221 gtttctctgc tctattaaaa gttcttttct agctttgata gtactgctcc cttgttgaaa
     8281 catagctaca tactcaatta atgtctcaat agaaagacct gcacctctca tacactttat
     8341 gaaattaacc cactcacaat cttcttctgt aaaagaccta attccacttt tatttctatt
     8401 tacaggtggt attaaaccaa ttcgttcata atatcgtaaa gtatccgctg aaagctcgta
     8461 ttttttacta acttctgaaa tcgtcataca tatcttctcc tcttgatatt gtatagattg
     8521 aattatttct caaaggtttc tgctacattc tttaaagcat ctataatttt aatgtgttga
     8581 ggacagcttt cttcacattg tttacattca atacaatctg aggcctttcc ataagtttta
     8641 gtataattat tatagtagac catctgtgtt gaaaatccag tattaagagc ttgtttttct
     8701 gcattgtaca acgcaaaata gtttggaata gcgatatttt ttggacagcc ttctacacaa
     8761 tactgacagg cagtacaagg aactaaaatt gattcgttga taatctctac tgcttcatct
     8821 ataatattat attcctcttg tacgaatggt ttgaaatttt gcatatatcc tgtattgtct
     8881 aataattgtt ccatatttga catcccactt aagaccatca taacattatc tttacttgct
     8941 gcaaaacgta ttgcccaaga tgaaattgac atatctggat tgtagtcatt caataatttc
     9001 tctgcttttt ctggtatctt tgcaagtgta cctcctttta ctggctccat aacaattaca
     9061 ggcttattat gcttctctgc cacctcgtag cattttctag attgtatgct ttcattatcc
     9121 caatctaaat aattaatctg taattgcacg aagtctactt ctggatgctc actcaagact
     9181 ttatccaata attctgcact atcatgaaaa gaaaaaccaa tattctttat ttttccttct
     9241 tttttctttt cttcaataaa cttaaagcta tcaaattttt tagctacttc ataatgtgac
     9301 acgcctatat tgtgtaaaag atagtagtca aaatagtcta caccacattt ttctaattgc
     9361 tcattaaaaa tggtttcttg ctcttctttt ttctttaaag ccattaatgg aagttttgta
     9421 gctatagtaa aactctctct tttgtgtctt tttactagag attctctaag tgctatctca
     9481 ctttttccca tgtgatacac ataggctgta tcaaaatatg taaatcccct ttctaaaaaa
     9541 gtgtctacca ttttatttaa atgttccata tcaatagttg taacatcatt ttcatctttt
     9601 attggtaaac gcattaatcc aaatcctaat ttttttgcct ccattgtttt acttccccct
     9661 ttaaaacttg tttgtttttt ctctctactt taactattct tacaagtata taattcttgt
     9721 ataattatac tttactactt ggagttaact tcaagtcaat agattttttt atttttaaga
     9781 caaatattat aaaaatacag ttctttaata ttaatccatt acatgtttct ttcaccctcc
     9841 aattctcaaa cttcacaaac tccaacatgt aaagaaatat atactttaag aaattcagtc
     9901 cattaatatt actacaaata ctttttagat atgtttaaaa agaaatttca cctaaaagat
     9961 ttagaaatag gtcttgtgtc tattttaaaa tatataaaac agtggtaaag acttatttat
    10021 aagataaaaa atgaatagtg tatacccttt ttaaaaagtt ttttcctttt tatatttgtg
    10081 atacaatgtt aaatataaaa aggaaaattt tgtattaaaa aaatcttatt tgttgatttt
    10141 gtaacttcaa actcagcatc aatttgattt taagattata ttttattaag tatgctaaat
    10201 aacaaatttt taaagattgt gaggtaaaaa tatgaataag aaaaatttag ctatattaat
    10261 atcaggtatt atgttttttt ctagttcttc agcaattttt gcagacaata ctacaaagca
    10321 agaaagatta ataggtaaaa caagatatga aacagcagta gaagtaagta aattgggatg
    10381 ggttcaatca aaaacagcca ttatagttaa tggtaattct attcaaagtg cgctttgtgc
    10441 aaatccattt gctaagctaa agaatgcacc tatactttta gtaaacaaca acagtattga
    10501 aaattcaact aaggctgagc ttaaaaggct tggtgtagat aatgtgtaca ttgtagatag
    10561 tggaaatagc atttcttcta aagtagaaaa tgaaataaaa tcacttaaca taaaaataaa
    10621 taaaatagta ggaaacaata tatatgaaat gtctacaaat gtacttaaag aaatagataa
    10681 gataaaaaag gtagaaaatg ttgcagttgt aaatggacta aagggactat ctgatgcagt
    10741 tagtatagct tctccagcag caatacataa tatgcctata atattagtat cagatgaaaa
    10801 cagcaatgat agtagtataa atttcttaaa caataggtct ataaaaaaat cttacatagt
    10861 tggaggaaat aagattttat cagacaatac agtaaagaaa tatccaaaca gagaaagaat
    10921 cagtggttca gatagaaaca atactaatgc aaaaattatt gataaatttt ataaagataa
    10981 aaaaataaat aatgtatttg tagctaaaga tggtattaac caagaaaatg cattagtaga
    11041 ttcattatca ataggagtac tagctgctaa agaaaattct cctataatga tatctaatgg
    11101 tagccttgta aatagtcaaa aagaatttat taaaaataaa gatatagatg aaataacaca
    11161 agtaggagga ggatataatc aaaaagcatt tgaagatatt ttaaaactaa aaaatgtgaa
    11221 cataagaaat aaaactaaaa aaatatcttt aatacaggct aaagaaatat taaaacaagc
    11281 tcttccagga agcaatattt tagtttgtta ttatgatgaa gatgataatg aatatgactg
    11341 caaagttata aaggataata tcctatatga gatagaaatt gatgcctact ctggtaaaat
    11401 aatagaaatt gaaaaagatg atgacttaaa tgataataat ttaggtacac aaaatatatc
    11461 attggataaa gctaagtcta tagcattaga tgctgttcct aatggtaatt taatctattg
    11521 taaatatgat gctgaagata acgaatatga ggctaaagta ataaaaaata agaaaacata
    11581 tgaattagag ataagtgcat ttgatggaag aattgtagat attgacatgg acaatgattc
    11641 tgatgatgac tttgatgata atgataagaa taactctaat actaatgaca acagttctat
    11701 tattagtgct gaaaaagcta aatcaataat gctagataaa gtgcctggtg ggaaaatagt
    11761 aaaatttaag tatgatgaag atgacaaaga atatgaaggt gaaataataa aaggaaaaac
    11821 tgaatatgag ataacaatta atgcaattac tggtaaaata ttagaatttg aaatagatga
    11881 ttaataaaaa atatatattc tattaattat tacaagcaat aaagccataa atattgaaaa
    11941 tttcaatatt tatggcttta ttttattttt tttaatttaa atccacatta tgccattata
    12001 tatacctatg cactctttta aattaaaatc ttgtccatca tcccaaacct aataaagtta
    12061 gattttaaac aatatattgt gatataagta atttgtgtag aattattgcg tgatttactc
    12121 atgaagaaac aataaggtta atatataatt tgactatatt aagacgaaag gagacatata
    12181 caatgaatac aatattaaaa gtaaataaca taactaaaac ttataataaa gggcaacagc
    12241 cgagtttaga taaggtatcc tttaatgtat cagaaggtga atttataggt ataatgggag
    12301 cttctggctc tgggaaaaca actctactta atgtattatc tacaattgat aaaccaacaa
    12361 gtggagatat tttcataaat aatataaatt tgaaatcgct taatgacact tgtgctgctg
    12421 attttcgtaa ggataattta ggctttatct ttcaagagta ttttttacta gatagtctta
    12481 caattttcga gaatatttca gtacctctta cacttcagaa actcaatcca gataaaatag
    12541 aaaacatggt aaagagtcta gctgaacgat ttggcatagc ttctcaatta aataaatacc
    12601 caaatgaatt atctggtggg caaagacaga gagcttcagc agttagagca atagtaaaac
    12661 atcctagtat tctctttgca gatgaaccaa caggagcttt ggattctaat tcagctactg
    12721 aactactcca ctcactacag gaagcaaatg aggagttgaa aacaacaatt ttaatggtaa
    12781 cacatgatgc ctatgtagca agttttgcaa atagaatttt gatttttaaa gatggtcata
    12841 tcattaaaga attaaataaa caacagaata atagacatac attttatgag ttgattattc
    12901 aagaaattgc aaaattggat actaaaagat aggaggataa tttaatgaac attttttata
    12961 cggcaataaa gaatcttaaa aaaaactttt cattctattc tctatatcta ctttcagtct
    13021 cttttgttat tactgtattt ttcgcattta cttcattttc tgttaataag ataatgctag
    13081 aaaaaatttc aactgatggt agagttgaat ctatgtgtaa tgttatttcg atatttttga
    13141 tggcatttgt catcttctat atggcatact caaatcgatt cttcttgaga cgtcgtacaa
    13201 aagaattagg tatatatgct ttacttggct atcgtaagtt tacaatatta tcgctactta
    13261 cctttgaaaa tgtattaatt tgtagtggag cttttattat tggattggta ttaggagcat
    13321 tggcacataa gggtattgtc tttggaatta cagcactact taatttatca attgataatt
    13381 cacagatacc attttttaat ataaatgcaa taaataaaac aattatcttt atagcattag
    13441 ttgttttagt cttagctgta tcaaatggta aattttttta taagactcca cttgtaaaat
    13501 tgataagata tgaaaaaagt gcagaaaaaa aattaaagtt cagaaaaatt ccagccttta
    13561 tggggtttat tatgatttta agcgggtatt gtttatcact agatattctt cgtggagcta
    13621 aatcagtatg ggtaagtatt ggattttatc aaatgggctt gataactatg attatcattg
    13681 taattggtac agttctcttt atcaattcct ttcttccata tattatgcaa aatagtaaaa
    13741 aaaataaacg tactttctac acagatatga agataatagc tattccaaac tttatctatc
    13801 gtatacgttc aaactctaaa actctaatta tgcttacatt actctctgct actactttga
    13861 cagtttcaag tgttatggca ctgtccttat actaccctat tgcatctgtt tcgcgtattg
    13921 caccttcaga gatagaattt agagtagaaa aaaataaaca aatagaagat gtaaaacaaa
    13981 ttgtcagtca atatacgtct aatacagatg atattatttt tacacagaca aatatttata
    14041 aggtaaaatc ttcatcaaaa gaccttccat ttgaatatga aattggtaca tctaagaata
    14101 atttatccaa tagtagttct tctaaagaag ttatttcaag agaatcagga tttgaatgta
    14161 tatctttttc tgattattat actttattaa aggcacaaaa tcgaacaaag ctactaaaac
    14221 aacttacccc tttaagggat aatgaatgta ttttaatgaa atatcaacca aataatgatg
    14281 gatatagtga aaatggaacg atttatccat tacttgttaa tggtaaagaa acacctgtag
    14341 ctgtaaggga aacatcatta aacaatgcta tttcttttgc caatagcgtt ggaacactta
    14401 tcgttaatga tagtatatat gagcaaatag cacaaaataa attaccaatg acaagaattt
    14461 taagtattaa tggtaaatca attaaaaata atgaaaattt atatagtaat atttcagatt
    14521 atttagatgg aagcccttat ttacaaggat attctcacag gataaatgat ttattatcaa
    14581 ttaatagttc tacatttctg ttaatcggat ttcttgtggt attattcttt attgctgttg
    14641 gaagtatttt atactttaac aatatatcag caatttcaga ttcaacatct gattataaaa
    14701 ttttacagaa aatgggttat actaatatac aaattaaaaa aattattaaa aaacaagtat
    14761 tgccattttt taatattccg tttttacttg gtatagtgga ctgtatattt gctaccttgg
    14821 tttataagac tgctttgatg cagaatttac ttggaaattc actttcacaa tacatacctg
    14881 tgattattgc aattttctta acttctgtag tatatttgat atattacata cttactatta
    14941 aatcctgttg taaaattgta ttaaaaaaat aatatttgcc aaatcgtcaa gcttaaaggt
    15001 gtgacgattt ggtatacttc atttataagt atttgatata atttaaaact ataaattctt
    15061 aagtttgatt tagtcataaa attaatataa ttaactcata aggaggtgta taagattgac
    15121 aaattttaat ttaaaaatac tatttttagg aaactacatt ctatcacttc tcttcatggg
    15181 aatatctaca agcactgaaa cttatatgaa tattcaaagc attattccct taataatttc
    15241 aacattatta ctcatagaca tcacatatat aacctcttgt aatgtccgag aaaataacat
    15301 tatttcattg ttttgtggat tactagcact agacagttgg tatttactct tatcatttgt
    15361 aaccacacca ataggcacta ttatttttaa aatattaagc acagttatag tgtatgtctc
    15421 tattaaattt atatttttat ttttgtttca aggtagtgga tacaatttcc gtaagataac
    15481 agacatttta ttgcttatca cctgcattgg tgcaattgta ggagcattta tttctaacag
    15541 aacgtatgct tgtatgtacg gaatacagtt tgttgttagt ataataagct ttttctatat
    15601 tattttttat catttgaaaa gggttgtttt tgtcataaaa agcgaaaaaa aacctatatc
    15661 gatttcagta tttataacaa ttataggatt ccttacttat tattttttta cactaagaat
    15721 atcaaatcat attggtaatt ttggaatcta tctaactgta ctggtatttt ttatgagtat
    15781 tcatggtatt gcacttaaag aaaatgatag ttttcctatt tcaacagttt ttaacataaa
    15841 acaaagggct atatttcttt ttataagtat tttagtaatt agcttggtcg tactatttgc
    15901 caatgggaat ttttctataa tattaatagc tataaatgtt ttatttatgc tcattttcct
    15961 ctgtaacatt attttggagt ttaaccttag agatgaaaaa aataaagtag ttaaaaatag
    16021 caagtatact tttgcattag aaaaattaca acaagaagaa caactaaaaa cagagttttc
    16081 taatttttta catgatgaca tattacagaa cctgctttca ataaaaaaca tgatgagtaa
    16141 gtcatatcgc ccagatgttc aagatattat atatgaaaca cttaacaatc taaatacatg
    16201 tataaggaag caaatgcaag actatcatcc tgttatttta aaaaatttaa cagtaaaaga
    16261 aaattatcag aatttaattg aaagtgtttc ttcatccttt ccacaaaaaa atatttgtgt
    16321 ttcatttaag tgttctgata ctttattctt agttgaacca tatgatatat tgatatatcg
    16381 actgattaaa gaattattga caaatgtgta taaacattca aatggtagcc atgcatggat
    16441 agctttagca ctagaaaaaa atataataaa attaaatatt agtgataatg gtgtggcaaa
    16501 tacatctttt aaaacgcctt tagatataac taaaattact tttgatacaa taaaacacaa
    16561 agggattata tcaattaaag aacaagtaga aaatctagga ggaacgataa gtgtttctga
    16621 taatatacct catggagttt gtgtacagat tacaatccct atgaaaggag atggttctta
    16681 taaatatttt gttagttgat gaccatatgc tttttgctaa aagcttgtca attgcactag
    16741 aagaatacaa agaaatcgaa cagttttatt ccacacagga catccataat ctttcagaaa
    16801 tgattcaaga taaaaaaata gacattgtct taatggatat aaaccttaga ggcttaagtg
    16861 aatgtgatgg cttaaaagta gcttataatt tattagagac taattcaact attaaaataa
    16921 taatcttgac tggatacgac ctcccagttt ataaacatga ggctaaaaaa ataggtatct
    16981 atggattcct caataagaat attagccctg atagtttaat tgattcatta ttaaaagtct
    17041 atcaaggggt aacttgtttt ccatctgaaa atactgaagt aattattgaa gatttaacta
    17101 ctatggagaa aagaatttta cagcttatct gtagtggtaa aaaaagaaaa ataattgctg
    17161 atgaattgta tataagtgaa agaacattat caaatcattt acaacacata tatgacaagc
    17221 tagatgtttc atcatcagtt gaggctatta caaaagctat taaaatgggt tatatttctc
    17281 cagtatatta aaaaaagtga gtgtctcaaa ataaatcttc taactcataa agcactctcc
    17341 tttgtattaa atcaaatata ttttcatcat ttaaacaatt aagaagaggc ttctctctat
    17401 tttgagacaa ctccatttta atacactatt tatatttata tataaacata ttttaaacaa
    17461 ctaaaaagca actaatatct caatacatat cttcataaca catatcacca taattacaag
    17521 cagataaaga actctcctca tttaaatacg actcaaattc atacccaaac agaattgttg
    17581 gttctatata tatttccata tctgtatatc tccatcttct tacctttgcc tctataacct
    17641 tgtaaaaatc ttctaaacta tacccttctt ttaatctttt atctataagt gccttagtta
    17701 tactcaaatc agctttaaaa ttttttcctg attcactatt taattttctt ataataattt
    17761 catgtatgct ttctggattt ttgtaagtag aatcttcagt caaatcttca tccgaatttt
    17821 caaaggtatt tttatttaaa ttctttttat ttaaattatt ttttaataat tcttttttct
    17881 ttgtctcatt ttctgtttca tacccagtat gaattatcaa atcataatct ttgtctatac
    17941 tctcttgatt tatacggctg tatatgttgg tattactgtt cttaaacaac tcatttctat
    18001 gtttatattt cattgttttt tggttattgt taatagttgc atcaatgtat ttctctatat
    18061 ttttgaaaat atcaccattc tttgttacaa tttctgcatt actactttca gaacaaacat
    18121 aactatatac agaacaacaa tttcccttta ctcccctact tattaatctc aatataccta
    18181 aatctataaa ttcatttaca agtctactta ttgtagatat agatacatca aggtcagatg
    18241 cagctttcct tactgtcatc ataaattgtc ctcttggcag agttcctttt aatctttgta
    18301 tattttcttt tctcattaca tagtgattaa accttattct atttacatct cttttttcat
    18361 taaaatataa tactgctgaa tttaattgat aaaaactatt actcatattc ccactcctcc
    18421 aatttaaata gataatttgt tgtaaagttt ttagattttt aaataactta ttaaatgatt
    18481 atttttatta aaaagtattc tttgatattt tcattttaaa gttttaagta taataagtta
    18541 atagtgtaga tttaactttt ttatttaaac aataaactta atattatttt taatataaaa
    18601 attttattaa atttagtatg taatttttta atcttatttt attccatcta tactcttatg
    18661 ctaagtaata actttgataa tatttcccta acttatgttt caactggttt atattgcaat
    18721 ttatttaatt acagcttatt tgcataataa ttcgtccata tcataagttg tgtatttata
    18781 taaatacaca attaaaaata aatcactatg gtatactaat tattacgaag actatactta
    18841 tatgcaaaga caaatataag gcatcttata tcttttatta attataaata tcttttatta
    18901 attataaata tcttttatca attataaaaa attaaacgct atcttattta tcataaatta
    18961 caaaaacata aaattttatt actatattgc tataaattaa ttgatacaca ttttacacaa
    19021 taaggagggc tatatatgac aaatgctaaa agtaaaaaag aatatctaaa tcgtatttgc
    19081 aaagtgcaag actatataga agaacattta catgaacctc tttctcttga tgaactttct
    19141 aatatagcag gtttttcaaa atttcatttt catagaatct tcaaagcaat agaaaaagaa
    19201 actttggcac aatatgtaaa taggctaaaa ctagaaaatg caactgcttt tcttattcat
    19261 agaactgaca tgactgttac tgatattgca cactactttg gctttactga ttcagcagtt
    19321 ttctcaagag catttaaaaa ttactataaa ataagcccta taaaatatag aaacaattat
    19381 agcaagaatt gcaaagaacc atataaaatt tctcagtaca atagaagcat atcaaaacct
    19441 aaatgtaaga atactagaga ggttaaaggt gaaattgaaa ttctagaact tgataatatg
    19501 aatactattt acactaggta tactggttca tatggtaatc taaccagtac tttcccaaaa
    19561 cttttagaac ggttattcac atatgcaagt gagcagaatc tacttggact tgaaaacaca
    19621 caaatattag ccatttatca tgataatcca caatttacac aggagcatca attaaggact
    19681 agtatttgca tgaccattcc agataacatt gttgtcaaag agaatgacaa tcttggcaat
    19741 atgataatac cttctggaaa atatcttgtg ggacattttg atatttcaaa atctgaatat
    19801 agtgatgcgt gggattttat gtataatgaa tggctatcaa atggtaatta taagctacgt
    19861 gcctccttcc cttttgaagt atatcttaac aacccacaca atcaccctca aaatagacac
    19921 ttggtcgata tatatttacc tatagaacct ttttaataac ctcacctaat ctattcttgt
    19981 attaaggatt aatatatccc tattttacgg tttgataaaa ttgataaaat gaggtgaacc
    20041 aatgaaagaa ataaaaatta atggagcaaa gatacacaat cttaaaaata tagatgtttc
    20101 tatccccaaa gataagcttg ttgttgcaac aggtgtcagt ggctcaggta agtctagcct
    20161 tatgtttgac attgtatttg aggaagggcg taaacaatat ctgcaatctt taggtatact
    20221 tgcaggtatt gatagtgaag ataagtttga taatattcaa ggtttagctc ccactatagc
    20281 tgttcaacaa aatattattc gccagagcaa tcctcgttca acagtaggca caagaaccaa
    20341 tattctcaac atgcttacac tattgtattc agtggaaggt caaataatgt gtactatgtg
    20401 tgatactcct gtaagctata atctaatttg caataattgt ggatatgagg aagaacgatt
    20461 aagaccaagt tatttctcat acaactcttc agatggaatg tgtatgaagt gctctggaca
    20521 tggtgcatat tttgagatta atattgaaaa attagtcctt gataagcatg ataccctaaa
    20581 acaagtctta gatagagcaa agattacacc tggatatttg agagtatttt ctaaaaaatt
    20641 caatgattat ttagacatac aattcttaca actacctgaa gaagtaagaa atgaagtact
    20701 ctatggtcat tatgaaaatg gtaaaaaaag ttcctcttta tcaaaggtat ttcacaatcg
    20761 ttacaaaagg ggcgaagatt taaatggagt ttatactatg actgcctgtt cagattgcaa
    20821 tggttttaaa attggtgaag aagcacgtag agtactttta aacggaaaac atattggaga
    20881 acttggaaaa atgacgattt tagaaattga tgatttcctt aaactcttaa taaatcaagg
    20941 taatttaaac acatttggta caaatcttgt aaatgatatt ttatccaaaa ctcaacattt
    21001 aatagaatca cgattaggtc atctatcctt ataccgagaa atgtcaactt tgagtggtgg
    21061 ggaaatgcaa agactatttt taaattcaca tctcgattca gagatggact cattaattta
    21121 tgtgcttgat gaaccaacag ctggattgca tgaatcagaa aaaatagaca tcttaaaatc
    21181 aataaagaaa cttaaagatt taggcaatac ggtcatagta gttgagcatg ataaaaacac
    21241 aattaaaatg gcagaacata ttattgatat tggtccaaaa gctggtgttg aaggtggtca
    21301 attagtatat caaggagatt tagaaggtct acttcaatcc gacaaatcac tcactggaca
    21361 gtatctatct ggaaaatatc caatgcctaa tagaacaagc aataaaaaca ttacacatac
    21421 tgataaaata ccttgtataa ctattcaaaa tgcaaataca aataatctta aaaatgtaac
    21481 tgtatcatta cctttgggtg ctatggttgg aatatctgga aaatcaggta gtggcaagag
    21541 ttcacttata tcagacaccc tacttcctct tcttagaagc caatttaata accatactat
    21601 caataatcaa ataaattctt cagagagtga aataggtgag gaagacgatt atgcagttgt
    21661 tgaaactatt gcagatagac tgattggtac taaacatatt tctggatatt cagagatatc
    21721 tcaatctcct attggcaaaa atatgaactc aactcctgct tcttatattg gtatatggga
    21781 taagatacgt acactatttg caggtcagcc agaatcgtta gaacgaggat ttacagctgg
    21841 tcatttctca tttaactcca aaggggcttg tcctaaatgt agtggtagtg gatatgaaaa
    21901 ggtctggttg ggaaataatc taagtataga ccatatatgc agtgaatgtc agggaaaaag
    21961 atttaatgat gaatctttat ctattaaata taaaaataaa aatatacatg atattttaaa
    22021 tatgagtgtt tctgaatctg tacatttctt caaggatatt ccaaatattg tttcacactt
    22081 aaatgtatta gaacaaatag gtatggggta tataaaatta ggccaaccta caccaacttt
    22141 aagtggtggt gaatcccaaa gaatcaaact tgcaaaagaa attggtaaaa aacgaaatgg
    22201 taatatcctt tatgtattgg atgagccaac tactggtcta agtctatacg atacttctaa
    22261 gttgattcaa ttattagatg aattggttga aaatggaaat tctgtaattg ttgttgaaca
    22321 tgatatagat gtattaaatg tttgtgactg gattgttgag cttggtccag aaggtggtga
    22381 caaaggaggt tatataattg cagaaggttc tccaaaaact ctaaaagaaa atccaaaatc
    22441 tataacaggg aggtatcttt aatgaataaa aacaattgct tagatttaat atttgaaaaa
    22501 gtaaatcctt caagtgttgg catgagttca gaaaaactct cacaacttga acctttaata
    22561 atctcacaat atagtaatat aaatggaata attattgcaa gaaaaggaaa agttgtcttt
    22621 gaaaagtact ataatggata taatgctgat gatacttttc atgttgcatc agttacaaaa
    22681 agtattatat cagccttaat aggtattgca atagataaag gatatataaa agatgttgac
    22741 caaaaggttg ttgatttttt ccctgaatat acttatagtc cttctgaaat atgtaaaaaa
    22801 gcagtaacta tacgtcacct acttacaatg actgcaccat acccttttaa aaatatgact
    22861 gagtctttag aaagactttg caaacaaaaa gattggataa aatatacctt gaatcaactt
    22921 ggtcaaaaag gcgaaattgg tactttcaaa tattcttcag ctggaacgca cctactttca
    22981 gctattataa caaaaactac tggaaaatct gctcgtgaat ttgctaatga atacttattt
    23041 aaacctattg gtatgaaagt tataccagat tatgatatga aagattttgg ttttgaagat
    23101 ttctttggaa aaaatcttaa gggttgggca aaagatagac agggttattc tataggtggc
    23161 tggggactta cattgagtgc tagagatatg ttgcgatttg gttttctgta tttaaatgat
    23221 ggtatttgga ataataaacg tattatatct aaatcgtgga ttgaagaatc agttaaaatg
    23281 aatagtaatg aatacggtta tctgtggtgg ctatgtaaaa aagatggtgt tttgtggtat
    23341 tcagcaatgg gagctggagg aaatgctatt tgttgtattc cacaatatga cttagtaata
    23401 gccataccat ctaaaatagt gtttaaacct cgtgatagat gggagtttat tgaaaaatat
    23461 atacttactt ctataataga ttaattactc tttcataaat taattactct tagataacca
    23521 aaccctccat tataacacca gacttttgtt tggtgttatt atgactaata ctatatatct
    23581 aaaaggagtt gaatactaat ggacacttac acgacatcag aggtagcaga tttaattggt
    23641 gtacatccta atacagtacg tttatatgaa gaactaaact taatacctaa acctaagcgt
    23701 aataccaatg gttatcgtat ctttaatgat tttcatatag aacaatttag atttgctcgt
    23761 acagccttga aaattgaagt ggtacaaagg ggattaagaa aagatgccct tgagattgta
    23821 agaacatcag cgaataggga ctttgataag gctattagtc ttgtaaatca atacttgcaa
    23881 caattagaaa tagaaaaatc taatgccgag gaagcactta ctattgttgc acagatttta
    23941 aatggaaata ctccagaaac aaataaactc tttttaacta gaaaagaaac tgcagaacac
    24001 ttaaacgtta caatagatac tttaagaaat tgggaaatga atggtttgtt aaaagtaaaa
    24061 agaaaacaaa atggttatcg tgtatatact gatgaagata taaaacgact taaaattata
    24121 agcgcacttc gttgtgcaaa ctattctctt tcagctattt tacgaatgtt aaatgcttta
    24181 tcttctaatc ctaatactaa tattaaaact gtccttgaca ctccaaaaga tgatgacgaa
    24241 attatctcag tgtgtgacaa actgataact tcattgaaag aagctgaaca taatgcaaaa
    24301 accatgaaat ctcatcttca aattatgaaa aaacaatttt cctagccctc cactctaact
    24361 ccagactttg atttgtgtgt tatccttaag agcaagaaaa attcaacaca caaatcaaag
    24421 gaggaatagg atggaaaaca aaaatactaa ttctacatgg aagaaaaaag tcattctttt
    24481 tttagcaagt caatatattt ccttatttgg ctcatcactt gtacaaatgg caatgatatg
    24541 gtatgtcaca cttcagacat catcaggtgt atgggtaaca gttttaactg tatgctcctt
    24601 tatacctcaa atgataattt ctttttttgc tggtgtatgg gcagactact acaatcgaaa
    24661 ggtactcata attgtatcag attcaacaat tgctatatct acattagttc taattctatt
    24721 tatgatgtca tcaaatggca caacaagtga attaatagcc atatcagtaa tatctgttat
    24781 acgttcacta ggaagtggtg tacaaacacc tgctgtaaac gcaatgattc ctcaacttgt
    24841 accagaagaa catttaatgc gttttaatgg tataaatagt agcctacaat ctatagtaca
    24901 atttatagtt cctattgtag ctgctgcaat acttggattt ggtaataaaa taaatcaagt
    24961 tcttttaatt gatgtattta ctgcaattat tgctattgta ttattgatat tcatcaaaat
    25021 tcctaaacat gatataccaa aacaaactga aaaagtctca ttttttgcag atatggtatc
    25081 agggataaag tactgttttt cagataagtt cattagaaaa gtgcttttta cttatggagc
    25141 attcatgctt ctttgtgttc catcaggatt tcttatagga cttcttattg aacgaacatt
    25201 tggcaagggt tatatatacc tgtctattat tgaaatgatt ggatttttag gtatggtttt
    25261 aggtggattg attataggag tttttggtgg ttttaaaaat agaaataaga ccttttttct
    25321 agcaatatta tcctatgcta ttttttcaat tattctaggt cttgtaacac aagcttggca
    25381 attttttata tttatgtttt ttacgaattt ttcaattcca atcattcaag catcagctat
    25441 gactatgttg caagagcatg tagcaccaca aatgctaggt cgagttctta gcttacctat
    25501 tattatatat actggtttta ttcctcttgg tatgatgatt ttcggaccaa tggcagatgt
    25561 aatttctatt aataatttaa taatcatagc agggattata cttttatttt ttgctttggc
    25621 tatcccctat tcaaaacaat tttacaagca aggaatatct aagtcaaatg aatccgagta
    25681 atatatttaa atagtttaac tcgatttatc tggttaaaaa tgagggggct taaaatggac
    25741 tttttaattt atgaagaaaa ttttttatat gaaatcaaat atattttatc atttcaacat
    25801 tgaaaaatag gtttatcttt attttgagcc cctctcttta tagcaaataa tattttgata
    25861 ctatctttat taactttata taataatacc tttacttgta ggttataatg atgaaattta
    25921 tcaaatcata taataatacc tccatttata tcataaatta aatttatagt acaaatggag
    25981 gaaaataaag tatttatata gtttaagtac ctattaagtt ttccactaag tagctctaat
    26041 tgtactgaac ttcttactaa attatattga ataattataa aataaaacta actattacac
    26101 tacataaatt aaagtataca aattgcacta cataaattac actatacaaa gtatattaaa
    26161 tcacctatta aattgtccca attataatta tactgagtta tctactaaaa aataactttc
    26221 tttttttatc taaaataata tatatcataa accatactac acaatgtaca gccaaaatta
    26281 caatatcttt gataataatg acattaccat taataatctc cataatacca ttaaatgtag
    26341 caactgaagg cattatatag tataaattcc ctataccact ggtattaacc cctagaagat
    26401 atattaacaa aggagtacct ataaataaaa tcattactat tttaatataa acaattccta
    26461 ccataagacc ttctgacaac tttccaataa ataaacctat aagtgatgaa ataaatgctg
    26521 aaaatacaac gagtaacaac atcattatca tatctaatat tgaaatatcc aaacatataa
    26581 atacagttat cattgaagat aaacacccac ataaaagacc caaaaatact ttttgtataa
    26641 tatactgact tctactctgt ggcaaaactt catttataaa tgatattcca tcttcttttt
    26701 cagatataat attcattgca ttaaaggtgc atcccataaa catagcagta attaaaatca
    26761 tggcaataaa aacattttga aactctttta aaatactatc tttaggcaat attgtaacct
    26821 caacatcttt taaatcaccc tttagtctaa acatctctgg caatgtcttt ccaacatttt
    26881 ttatagcatt aatctcattt ccataaagca cagtttttat ccctttacca tctctaacaa
    26941 ctccaattgt gtctgtttta atatccttaa tttcacgtat taaatgcatt tttgtgtcat
    27001 atacacttac cataccatac ttcttcaacc actgctctgt ttgtacagga agtgtatcct
    27061 tcactatacc aaaacgtatc tctcctaatg aagatatatc aattgttccc ataaaattta
    27121 ttaaaatcgc cataacaact ggtaaaaaga aggaaattat acacatttta tcttttttta
    27181 tactctttaa ctggtaacgt aatcctatca agattatccc tccctaacca actctttatt
    27241 aaaaatatat tttacaagta aaaatagaac tattattgtc aaagaacaca ctacaaaata
    27301 aactaattct tttgaaaaat tcatgaaaaa cgcattttta agctgactat aaacatgata
    27361 gaagggatga tatttaatcc aatctatttc aaaagatgta tttgcagata gaaatatagg
    27421 tgtagttatg aatacagcaa ccacaagata tataagagaa aattgcttaa aatctctcaa
    27481 taacattgag catcctattc caatcaaaga cataataatt gataaaatac tagcttgaac
    27541 taatacttgt ggaagtattt ttattccttc aaaaaatcca atattaaata ctactaaaag
    27601 aactgtaaaa cataggtcac atatcaaaaa agcaaatatc tttgataata taaaaagact
    27661 tctttttaca ggtaaaatac cagtaatacg cattactccc atctgctttt ctttaaataa
    27721 taaagaggca atccctaaaa atccaatagc tacaatttca aagaataaaa tctcacaagt
    27781 catgtctttt ctttgtttta tctcagatgt atccttatca ttaatttgaa ttattgcatt
    27841 gttgtctgat attttgctat tgtggtcttt ttctgatgat aataatgaca atgcatagtc
    27901 agctctataa tcgtcaactc ctttaatact ttcatgtaaa attactttcg gcttactgga
    27961 actcatatca atacaaactg cacttgtatc ttcatcaagt tttgctttta attcacctat
    28021 agaagaaact tgattaatta atggagatgc actcatgctt atattctctg gattatatag
    28081 atatacatta ttgtgataac tatcttcatc cataaacttt acataaccaa aattaatata
    28141 acacgaataa agtatgagag agccaagtgt cagcacaaaa aatttaccat aaatcatcat
    28201 ccaaaaatct ttttttacta aggtaaaaaa tttagagtac attacactaa catccttcct
    28261 gtgtatttaa taaaaacttc ttctagtgta ggttcttttg aatgtacact taagatttca
    28321 tcatatttaa aagttattcc agattctaac tcttgtatat ctacaatttc aatatctcga
    28381 tttccattat atacaaattc cacttcaact ttattattgc tatttttcat ttttaaattt
    28441 ttgggtttat ctattaccat aatctttcca tctgcaataa aagccacatt gttacacatt
    28501 aaatctgcat ctagcatatt atgagtagtc aaaaagatag ttgttccttt ttttcgttct
    28561 tcttcaataa tcttacgaaa tagtacagct ccagatgggt caagtccact agtaggctca
    28621 tctaaaaata acaactttgg attacttacc aatgctcttg ccatactaat acgttgcttc
    28681 ataccttttg agtaggatga tacaggctta tttataaact ctttcttaaa ctctaacaaa
    28741 tctaagagtt catatatatc tctcctttga tttttaggat aaaaagacga aaaatattct
    28801 aaattatcaa ttgcacttaa attggtatat aaataaggaa attcaaataa aacaccaatc
    28861 ttttgatata aagattctgt gtatgcttta acttccttac caaaaaggga tacgtgacca
    28921 ttgtaacctt ttaaaacacc atttaagatt ttctgtgttg tggattttcc tgaaccatta
    28981 ggtccaagga atccaaagat ttctccttct tttacttcaa aattaagtcc atgcaatatc
    29041 tgtttatctt ttccataaga aaattttaac tctcctactt ttatcatcta tcataacctc
    29101 ctttatcatt tttttgtcgt ctatcccacc attttataaa tttaatttta agcggaatag
    29161 aaagcaatat tatcaatata atagtcaacc atgtatacca tttcataagt actccccctt
    29221 gtaaccttat ttaattacgc ttctattttt taatttattt aacctatttt atttctattt
    29281 ggtgaaatca gagtgtctct cttgtgaaat cgaagtgaaa taataaaaag cttaataaaa
    29341 aatcttgttt tcatcagaaa aacaagactt ttcattaagc tttttattag actttttcat
    29401 taagcttttt tcattaagtt tttttatttt attctcttta aatgcttaca tatacaaaga
    29461 aaaatatttt acatcagata cttataattt ataaattaat actaaattat tcatcaattg
    29521 gaatcataaa ataaaactct actcctctat cacgattatt cactccatat tctaaccctt
    29581 gcattgtaag tatttgtgaa acaattgcaa gccctagacc tgaacctcct gtacgctgag
    29641 attgaacact acgataaaac ttagaccaaa tctttgaaat ctcatcttgc tctataaacc
    29701 taccatcatt ataaatttca aataaaagca agccattttc acatgtaatc gataaatcta
    29761 aattaccatt atatacaaca tgcttctttg cattaataac tagattctct actacttgtt
    29821 ccattctcat tttatctgcc actacaaaaa cttcatactc tggcaaatca tatgtaaaat
    29881 aaaaattggc atctggtata tcaattaaaa gacgacctgc cacagtttca acaaactcta
    29941 taaaatcaaa acgctctggg ttaagtttag aaactcctgc ttccaatgca gataagtcta
    30001 ataatgatac aatcatattg ttcattctat cagtttcttc tattataaca ttcatataat
    30061 ttttcttttt ttcttcatca acttcatcca taagcccctc tgtatacgca cgtataatgc
    30121 caagtggagt cttcatttca tgtgaaattg tatctaccaa ctcctttctt tgttccaata
    30181 gcattctttc cttatttaca tccttttcaa gttgaatatt tgcactttct aggtctgata
    30241 atgcctgttg tagatttgta gacattgtat tcaggctcct agaaagttct ccaaattcat
    30301 cattagtatt tacagtacat ggattagaaa aatcaagttg ggacatactt tttgcagatt
    30361 tatttataga tgtgattggt tctgaaataa attttcccaa taagaaatca atactcataa
    30421 taaatataac cagtattata aaccaaatcc aaaattctaa tttagagttt atcgaaaatt
    30481 cttcaacaac cagataaaat aatataatta acgctcctga taatttagaa agtaaaaaca
    30541 ctttttttct aactgaatat agtccaaaat tttgtttaat ccaatttgaa atatctacat
    30601 ttttcagttt ttctataatc tttcttaaac tattcattcc ttaacctcaa acttatatcc
    30661 tgaacgaatt aatgtaacta tatggtgtcc ttcactacca agctttttgc gtaaggtttt
    30721 tatatgtgta tctacagttc gtgtgtttcc ttcaaaatca tatccccata tacaatttaa
    30781 aagtttctct ctggaaaaaa cttgattagg gttttccata aataaacgca ataattcaaa
    30841 ttctttatat gttaaatcaa tcaatttatc ttctacatac acttgccttg atgctaccat
    30901 aataaaaatc tttcctaaac tcaatgaatt tttgggaata tcagtagtgt atctacgtaa
    30961 caaggcatta acttttgcta aaagtacttt ggggctaaat ggttttgtta tataatcatc
    31021 tgcgccatat tcataaccct ttaatttgtc ctcctcttca cctttagctg tcaatataat
    31081 aataggtata ttagtcattt cacgtgaaac tctacacact gaaaatccat ctaaatacgg
    31141 catcataata tccaatatca ttaaatctat attgttgctc tttaatattg taagtgcctc
    31201 gactccatca tcagcagtta tacaagtata tcctcccttt tgcatatatt ctacaataat
    31261 actttgcatc cttttttcat cttctacaat tagtatttta gccatttcta tttatcacct
    31321 cttttttgta ggctttattt tagtttatat taatttattt atttttcaac tattttttta
    31381 tagtattttg atagtatttt ttatagcatt ttgatagtat tttttagact attcttaatg
    31441 gaattgatat catagataaa aatagcctac taactttttc tatgttagca ggctaaaata
    31501 tttctaataa taattatatt attttgtcac tattctattt tttcgattaa aatatattct
    31561 aaaacaaata tcttcaatta atgaataaat tgcacttgtt ataaagtaca gacctatggc
    31621 tactggagtt ctaacagtta gtataagact tgttatggta gtcaccacag ccatttgttt
    31681 gttaaacttt acttgagaat ttatttttaa atatggtata taattcacta aatttgaagc
    31741 taacataatt aatgtatata tacaaggtaa tataaacaaa ttatcaaatg tacctaaatt
    31801 agcaatccat ggaattataa ttgttgtaaa ttcctttgtc atatctaaac atacatgata
    31861 taaagcatat actactggca tttgaaccaa aactataaga catcccagca tactcttcat
    31921 actttctaca gaacttactt gtatttgctt ttccaattct ttagtatcat tcttatactt
    31981 ttcttttata tgttccattt tttcagctaa ttcttgctgt ttttttacag aaaatctttg
    32041 ttttaatgat agtggcataa gtactaattt aactattaaa gttataagta caatagaaat
    32101 tccaaaatca cctgtaaaat taaataatat acttaataat ccttgcaata tacttgaaat
    32161 gaaatccatt ttattctctc ctttttattt ttaaacttta gaatttataa tagattcatt
    32221 tcaaattaaa acaaactaca atacctccaa atatcatatt tctgaaaaac tatatactgt
    32281 ttaggcaacc gttcttcaga atatgtaatt ctatttacaa atatgtctct tattaaaaca
    32341 tacttaattt tatgatttaa gaggtcttta caaaaataaa atatacttcc aatgatagat
    32401 accaataatg tcatataaat tagtacatat aacatactat ccatatctaa tctcatcaca
    32461 tcacctcttc atcacttata tttattttta gacaatttca agtcctaaat aattccaaca
    32521 tttatattta gttttaaata attatagtat agaaaactcc taatg
//
LOCUS       DAMJVK010000040        31049 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.040, whole genome shotgun sequence.
ACCESSION   DAMJVK010000040 DAMJVK010000000
VERSION     DAMJVK010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31049
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.040"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            368..2602
                     /locus_tag="PC414_001942"
     CDS             368..2602
                     /locus_tag="PC414_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2464337.1"
                     /translation="MQNHNYIKMLNKKVEVLSKELDSRNFKLKTLNEIDFFSKIPNRE
                     FMYQHIDDCISKNIHIGIMLLNIDNFKLFNDIYGYLVGENLLITLTQNLLHFIPESKG
                     IVAYLKGEGFLIVLPNVSNKELNNMGATIIESARELKIRLPNEKEIYSNVTFSIGSTI
                     WNGEPNMTTLMLFNQVYSALKKAKSEGKNRYILFDFKDNSFYNKLENDKEFLSEISHV
                     LQTKQIEVFYQPLYNIKNNTIVGCEALARWRHPIKGLLTPDKFIPLFEQFGLITELDL
                     HLFEENCKNIRRWIDNNDVFVPICCNFSRLHFFNPNFPSMLKGITEKYSVSTSNFSIE
                     ITENILIENTNTIIQQLAELRSYGFSVSMDDFGSGYSSFGMLQNLPFDIIKIDRIFLS
                     HNLNDFKNTTILYSIINIARALGMLTVCEGVETEQHVEFIKSINCDIAQGYYYAKPME
                     CKEFEKLLSKENNSEKMFIKYSDIGKSLIEKIFDGFFIMQDMKKLRANVTEDIQWFDF
                     YNKTEITSFPKVIEHFEKHIKGKKFSILYKSITPHNTESDSLLISGEAVLSDEASEHQ
                     KYSNFYFSANCIISENKLLLAKLHLDLIKTAGYINEFESYSIPKMTNTHLDESNILSQ
                     FYSMLPVGIIRYDLFGDMIITYMNEEMFDIIGYTKEQFFGEMGGNLRAIVHPDDLDFV
                     YKKSLDMIDTNKIEPFLYRFIKRDGQIVTVMYTQCNLSAIDGRSIAQGMYVDVNKIKN
                     LTKI"
     gene            2633..3478
                     /locus_tag="PC414_001943"
     CDS             2633..3478
                     /locus_tag="PC414_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2464338.1"
                     /translation="MFKEIFLRTFPGFLIIRDSNYRIIFINDNLKNLIKSYMQDNPLG
                     MTNIEIAKKLPDNIAKFFTDSHNIQLDWEKNYPYDKISNWILEFKKDTTSYWNVLEYK
                     VDVDEKTYIITMANDITKLYEENKRNLHYSITDPLTGAYNRKYLNDRFDIFIGDYIVL
                     IDLDNFKMINDYEGHNVGDKILCDFVSLLNKELINSTSIIRLGGDEFIVIFSSDVDKN
                     YVYSQLEALRENFLKVFSKYKYLSFSYGVDTVKRNLKLTIVELDKKMYKNKEKNKKKF
                     DKNDY"
     gene            3718..5451
                     /locus_tag="PC414_001944"
     CDS             3718..5451
                     /locus_tag="PC414_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF2464339.1"
                     /translation="MSILLKKAPKLAKHIITSFYINRDINEVLKYLCENVTWIGPGEQ
                     EFLTSFNEIKNYFYAGQDEIPSCDINNDIFETVSEDENRCMVLGRYTVRTKENAQMIL
                     EVNQRCTFEIIEDRGKLLIKHMHISNPYGEMQLDEYFPTKIGTQSYDYLQRLLKEKTE
                     VIEMITNNINGGLKGSNDDSTYSFFYVNEGLPKILGYTYDEFMEMSGGSAVGAVYPPD
                     LPKALEDCERCFAKGPTYSSEYRIRKKDGTLMWVLDSGMKSLNSDGIVKINSIITDIT
                     QLKNIESELKLERERYRIALQNITDIMFEYDIENDTLIKYQRVEIDKKIELENFETKN
                     YSKLLESGKIIHLDDIGKLLEVLHGNLRETIEIRQVNSLTKNEWRWIRVQCSVIYDSD
                     HNPIKTIGVLKDITEDKSKLESLINQAQRDPLTQLYNQRVSQNLIEEYLCSSDSKNND
                     ALLVIDIDDFKTVNDTFGHLEGNEVLVAVSKILLHNTHDKDIVARIGGDEFTIFIKSL
                     TKDLIIKITNDILNDASKIKVKDNHKITLSIGIAFTDDSTKLYKDLFSKADKALYLSK
                     ADGKNCYSTYE"
     repeat_region   5777..6263
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=5909..5937
                     /rpt_unit_seq="atttacataccacttagttaatataaaac"
     gene            complement(6793..6897)
                     /locus_tag="PC414_001945"
     CDS             complement(6793..6897)
                     /locus_tag="PC414_001945"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDF2464340.1"
                     /translation="MDDYIYYYNNFRYQNRLNSMSPFEFRKYLSTERA"
     gene            7522..7767
                     /locus_tag="PC414_001946"
     CDS             7522..7767
                     /locus_tag="PC414_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464341.1"
                     /translation="MNQKKIKISIKMVIIGIFSVIIAFVLSRLFVEYIDELLRSNWQY
                     QLFESESSYIIFANCIMFSNILLEIFLIYICRKFRKI"
     gene            complement(8520..8666)
                     /locus_tag="PC414_001947"
     CDS             complement(8520..8666)
                     /locus_tag="PC414_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464342.1"
                     /translation="MVFETFDKTIELYPNAKPIFHSDIGFRLDDESFEEYLGRVFRKR
                     CKGI"
     gene            8699..9085
                     /locus_tag="PC414_001948"
     CDS             8699..9085
                     /locus_tag="PC414_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464343.1"
                     /translation="MNNNKFVAPNNVTLVERCYRYIKDRECSFSPNTIRNRKNMIKNQ
                     IEVFFKDMKLIDITPSILQSYINNIYNEHMLNSIKNQVDFIKSVLKESYRLKEISENI
                     CDFVTTPIKKVVQLLSFIQNKKLNYY"
     gene            9952..11307
                     /locus_tag="PC414_001949"
     CDS             9952..11307
                     /locus_tag="PC414_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2464344.1"
                     /translation="MDTKISINENMSLGKRFFKYLAPSVVAMWVFSLYTMVDGIFVSK
                     GVGELALAAVNISMPFINFIFAVSLLFSTGASTIIAIYLGKKDIKSANEVFSFNLVSI
                     IILSIIILAITFFNLDRLALFLGATESTIGMVKDYLGIIIFFNGFFIVSYSLEVIIKT
                     DGFPILATVGVIISALTNIILDYLFVIEFGWGVKGAGIATGLSQVFSTIFFLIHFLRK
                     NSTLNFSKFRIDFKTLRKIVFIGFPDSTTELSCGIVVLLFNLSLTKYIGENALIYYSV
                     INYINTLVLMTMMGITQGMQPLTSFYYGAGNIDNVKKLLKMGIKATIIASVAVFAICM
                     AFSGPIVSLFIHPEETMLFNEGVRVFKIFSISFLLVGINVIISGFFVSVEKPSISTVI
                     SLGRGLVIVVLSLISMILIFGGQGIWMTTIVSEFICLILSLVFLKKNFSTLDSNLNKV
                     A"
     gene            complement(11425..12093)
                     /locus_tag="PC414_001950"
     CDS             complement(11425..12093)
                     /locus_tag="PC414_001950"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HDF2464345.1"
                     /translation="MKEVLKLADEKFVMSFSGGKDSILALNRMLKKGYKPVALLTTIS
                     EEHGKSWTHNLEYNMLKQVSSNIGLPLLVAECGVEGYEESFERALIKAKNMGATICAY
                     GDIDIESHRKWDTDRCEAVGMKVDLPLWQESREELVYEFIDSGFCSVVTKVNLKHLGE
                     EFLGKKLTRELVEKIKNAGADPCGEHGEYHTFVVDGPIFKKPVEYEVKGTLIKDGYGY
                     LEIK"
     gene            complement(12160..12588)
                     /locus_tag="PC414_001951"
     CDS             complement(12160..12588)
                     /locus_tag="PC414_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDF2464346.1"
                     /translation="MTKLWAIIFALLAGGSTALEAFINGELGKSTTALVATFISLTVG
                     AIFFLASIIVTGDLKCLVSINGFNYKLLLGGVFGGLIIYFTVKAIPNLGVSNTLILTL
                     VSQVLVGFFIDSVILGQETMHLYKYIGVILLILGTFFIVN"
     gene            12695..13288
                     /locus_tag="PC414_001952"
     CDS             12695..13288
                     /locus_tag="PC414_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HDF2464347.1"
                     /translation="MNFCYNCSIDFGGDFMYTNFNIDFNNFNKKENAIKFMLMGVLLI
                     ILGLLCLTFKTLGIKLISWTFGIALLFFAYLNLKNINELKRYATKEEIKPSINIQWVL
                     IIACILLFVFPQKIQSIFSLFLGFYLIFNQLVALVNSKNNPYSKFTTWNIVKILFGIC
                     LILSPLFLSRFIVSIMSFFIILFGLVLFFSGNTARKY"
     gene            13407..14342
                     /locus_tag="PC414_001953"
     CDS             13407..14342
                     /locus_tag="PC414_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893027.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HDF2464348.1"
                     /translation="MDKFKYAFDNKRYHTWNYYLRNTFGEKVFKVSINAGFTCPNIDG
                     SLGYGGCTYCSKEGSGDFAGNPKDNLISQFYNIKEMMLKKWPHAKYIGYFQAYTNTYA
                     PLEVLKEKYETILELEDVIGLSISTRPDCLPDDVVEYLSELNKRTNLWVELGLQTIHD
                     STSKIINRGHDYKTFVEGVEKLKSKNIKVVVHIINGLPGEDYNMMMETAKAVGKMGVD
                     GIKIHLLHVIKDTPMEKMLQNGMLTLMEQDEYINLVCDQLEILPETMIIHRLTGDGKR
                     DELVGPIWSLKKWEILNKIDDTLKARNSYQGCKFV"
     gene            complement(14613..15557)
                     /locus_tag="PC414_001954"
     CDS             complement(14613..15557)
                     /locus_tag="PC414_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDF2464349.1"
                     /translation="MGEKSKGYVFIAIAGLLWATLGLFGKFLMGNGLTSEQVAFTRLF
                     FGFIVLGVYSSIRTPQILKINKKGIIYSVIIGIICQAMFNLCYFKAIDIAGVSIAAVL
                     LYTSPLFLAIFSKLCYKENITRSKLFSLILCFIGAIMAVTGGRLDFQGLNAFGLLLGV
                     LSAIAYALMPTISKNALKEFSSSTILVYSFLFGAIFMIPSSRPWEILNYAKDLDVLSC
                     MLMLGIVPAALAYIFYAAGISKGVELSVAGVVASVELVGSVIIGCTILGESFSLGKLF
                     GVMLMLISAVVALNLSYDEIRIFYKSNKLKQIEKTESI"
     gene            15875..17305
                     /locus_tag="PC414_001955"
     CDS             15875..17305
                     /locus_tag="PC414_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2464350.1"
                     /translation="MNLDLEFYQKILEASHDEICVSDDKGIIIYCNKAFEENYGLKKE
                     DILGKNVSFLEDSGYSTKSPIPIVLKTKTKFSLEQDTQTGKKLIITATPIFDENGHLE
                     FTVENCRDITELNNIKNKLEDTKKQVKKYKSEVETLYRTALRIEDTVIMDGIVMRPII
                     NTVNHVSKTDVSVLLLGESGTGKSSLARYIHHNSNRSNGPFITINCATISPQLLESEL
                     FGYTSGAFTGASTKGKVGLVELANGGTLFLDEIGDIPQNLQAKFLQLIQDRTFTPVGS
                     LKNKKVDIRIISATNVDLVSKVKEKKFREDLYYRLNVIEIKLPPLRERRDNLVEIIKY
                     YFNRYSSDFNLNKTISKEAMDAIANYRFPGNIRELQNIIQKILLTCTDNHITIDDLPN
                     ILTKNIHITNNGNKTHISQINKVIISDSKSTNYKNKNFDTLIKEYEKNIILDAYEKFG
                     SSYKVAKHLEISQSKANRLIRKYTNT"
     gene            17505..18089
                     /locus_tag="PC414_001956"
     CDS             17505..18089
                     /locus_tag="PC414_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HDF2464351.1"
                     /translation="MLMNTHFIMAKSILDNIDENKTFFISEKNFIYGNIKPDAFSKYK
                     LKKHYLDESFNMIVSKINYLCSLNIDSLSKYFSVSRLSQELGVICHFLCDFFCVAHSE
                     RWEFKHSLNKHVTYERELGAFAKDVDLSKIKCGTINSSFEKFFTKLYSDYKKKTDYMN
                     DLKFSAYTCNSVINYILDSILSNTIKSYHIANCG"
     gene            complement(18193..18972)
                     /locus_tag="PC414_001957"
     CDS             complement(18193..18972)
                     /locus_tag="PC414_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF2464352.1"
                     /translation="MIKHIFCDLDGTLYENGTITKEDIVAIEEIEKKGVQFNVATGRI
                     FKQAHNIIKDSLDMNGYYVCENGSFIYDKDYNVIFKRTIDDNLVKKVIDRFESSDAQL
                     YFKYKGDVIVGSDTTAFRHYSSDFIVDPDFEKRSSFDNLIGNIGVCSENLEELSRIEL
                     YLKSEFSEVLEIYFSGTYTLNIVPKSVSKRGSIEHVIKTLNVSPDEVATIGDSPNDIC
                     MLEGFKYSFAMSKAREDVKQSANYVVDSVKSAIDVIMEINS"
     gene            complement(19030..20286)
                     /locus_tag="PC414_001958"
     CDS             complement(19030..20286)
                     /locus_tag="PC414_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HDF2464353.1"
                     /translation="MKAEIISVGTEILLGDIVNTNSQFLAKELASLGIEVYHQSTVGD
                     NKQRLLECFDESLKRSDFVITTGGLGPTGDDMTKETAAEYFGQKLELHKPSLEVLESF
                     FVKTGKKMAENNMKQVYFPKDAIVLKNNNGTAPGAILKKDGKFIIVLPGPPREMKAMF
                     NESVKPYLQQFTNEMLVSKTLRLYGIGESNLELEILDIINEQTNPTVALYAKELEVTI
                     RITAKAENEREAFKLIKPVEEKIKSRVGKYVYTEGDISISEGETALEDAVSKLLVEKN
                     LTIAVAESCTGGLVSSSLINYPGISSVFLEGCVTYSNDSKMKRLGVKRETLEEFGAVS
                     EQTAIEMAEGIAKGLKANIGISTTGVAGPGGGTKEKPVGLVYTAIYINGKTIVKKNIF
                     NGDRRKIRLRATRDLLNELRIQLEKL"
     gene            20544..21443
                     /locus_tag="PC414_001959"
     CDS             20544..21443
                     /locus_tag="PC414_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDF2464354.1"
                     /translation="MKIAVIMGGISSEREVSLNSGKEIYNNLDKNKYEVVKVIIDDKK
                     DIFTKIPEDIDFAILALHGKFGEDGCIQSILETMDIPYSGCGPLCSGMCMDKNITKKM
                     LRDSNLPTAPWVLVKSVDEIDYDEIDNIGYPVFIKPNSGGSSVATFFIHSKDEVEEAV
                     RKGLEVDEFVMIEKYIPGGEYTSFILNGEVFPTISIKSDSGFFDYEAKYSVEKGAKEE
                     VVYLDEELQKRVNEISETCWKIFNCKAYVRVDMIISEGIPYVLELNTLPGMTQTSLIP
                     RSAAARGIKYSELLDKLIEYSLN"
     gene            21467..22915
                     /gene="ddlR"
                     /locus_tag="PC414_001960"
     CDS             21467..22915
                     /gene="ddlR"
                     /locus_tag="PC414_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator DdlR"
                     /protein_id="HDF2464355.1"
                     /translation="MIFSNLKLNDNEPIYIQLKNYISDMISKGLIPDNSKLPSTRELS
                     QLLQVSRNSVVLTYEELKSEGLIYSISGKGTFVKSKNKSSNTTWSLNWDCLENTYSKK
                     ANELDIIKSEIPWSSDLISFKSISPDGDLFDMEELKKSFLNRISLEGHKLLNYGYAQG
                     YKPLIDYLLEYMTNKGADISNKDILITNGFTEGFDIIMSSFTQEKDYIICENPTHNTA
                     IKIMKSHNLNILGVDINEDGLDFNMLEDNLVKYKNKIKFAYITPSYHNPTGIVMTPEN
                     RYKFYNLMKKYNIAIIEDGFNEELLYNSSHIFPICSLDNMNNGVVYIGSFSKILFPGM
                     RIGWIFADKKVINKLESVKRCRNIHVSFLDQGILFDYLSNGGFEKYIKKIRKFYGDKF
                     NFAYKCIKKYIKTEYILGDGGLHIFIKLKNIDTRVLLEKCYEKNVIFMPGDLFFTDNS
                     GFDTLRLGFSRLSFEDIEIGIKIIGETINEMF"
     gene            22974..23780
                     /locus_tag="PC414_001961"
     CDS             22974..23780
                     /locus_tag="PC414_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDF2464356.1"
                     /translation="MNLILGDNMSKKSKRRKINKLKLTIVITVLFLALLSIYEILFSN
                     PNKKGNIKSQETMQNKNNTTDDSKNNQSSTKKQEDNNIKPTFSYSSIPDNIKNKMIGK
                     SMPTDEPISFDSLSYLKLTYYGFDEKTHQGEMIVNSELAPEVVDIFKELYEKKYPIEK
                     IKLIDDYDAVDEKSMSDNNTSSFCYRTIAGTNVVSNHGKGRAIDINPLQNPQVSGNDV
                     TPKVSTVYADRSSTKFGMIKKGDDCYNAFVSRGWSWGGYWKNPDYQHFEK"
     gene            23806..25725
                     /locus_tag="PC414_001962"
     CDS             23806..25725
                     /locus_tag="PC414_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_071223118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDF2464357.1"
                     /translation="MNLMTLENISKSYSEKKLLENISLGINEGEKIGFIGVNGTGKST
                     LLKIIAGVEESETGNITKTNGIRIEYLPQNPTYNENSTVLEQVFNGTSEELKLLGEYQ
                     EILEKLNSNFTEELNKKLLSVHEKIDALNLWDLESEAKSVLTKLGINDFNQKIAELSG
                     GQRKRVSLASALITPCEFLILDEPTNHLDNDTIDWLEEYLNSRKGSLLMITHDRYFLD
                     RVTNRIIELDKGRLFSYDGNYSIFLEKKMERLSLEASIEDKRQNLIRTELAWVRRGAQ
                     ARSTKQKARLQRFDELINRDSFKPDENLDISVASSRLGNKIIEIHNISKSFEENTVID
                     NLEYTLTRTDRIGIIGKNGMGKSTLINILNGEIEPDSGYISIGETVKIGCFSQDDSHM
                     NIDMRAIDYVKEASDYIETSDGTRITASQMCERFLFNGTMQYTLIGKLSGGERRRLHL
                     LKILMTAPNVLLLDEPTNDLDIETLKILEEYLDEFKGVVVTVSHDRYFLDRICNKIFA
                     YEGNGKIHIYTGNYSDYLIYREIQGIEFEESKRESVKKDDSTTPKKEKPKNDKKLKFS
                     YNEQREFENIDSDIEKLESKIATLDESTAKFSTDFTKLQEILDEKSKLEKELEYKYER
                     WEYLNNLADEIANNK"
     gene            complement(25967..26314)
                     /locus_tag="PC414_001963"
     CDS             complement(25967..26314)
                     /locus_tag="PC414_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HDF2464358.1"
                     /translation="MSKSNDVLDIVFKITDGLEYEVAENRVVTILEKQDHKIQKFFRK
                     LKARIPKYKKIELDEYSSFVFLQIDGQRTVREIGKNLEAKYGEESHPLYERLLVFLNH
                     IEVNCHYIERLDI"
     gene            complement(26301..28229)
                     /locus_tag="PC414_001964"
     CDS             complement(26301..28229)
                     /locus_tag="PC414_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HDF2464359.1"
                     /translation="MNKKLPKGAYGEVSGKDYVPYITDKSRTGGNVAVLIIGIILAAI
                     FAASTTYSGMKAGLTVAAGIPGAIIGSAFVGAFARSKGILGKNLIQGMSSGGESVASG
                     FIFVLPAVILIGSQITFLEGLAVGVGGVLFGIGIAAIVHNYLIVEEHGKLMYPESMAI
                     SETLVASEAGGDSIKYMGIGFVISGFITVLTGSFLNVANNVMSLVGSKFYKWKFDIEV
                     NPLLLGIGFIVGLEVSLTMFAGSILSNFGIAPLIGYFTDMAKDGAMVWNNPAMPLNQM
                     DVGAISSSYVKYIGAGMMLCGGIIGAIKLIPTIIASIKETLKAKSSAGEGEEGSSIQM
                     ILLLGGVVIGFLAAFLISGNIVMAIIGAIISLLLSLLFVIVAGRLTGTIGTSNLPVSG
                     MTIASLVIVTLVFVIMGWTDLEANKSLLLFGSFIVVAIAIAGGYTQSQKVTYIIGGSK
                     NEMQRYFTIASIVGVIVVVGVILLLSDQLRATGDNVQFALPQANLMSTLTSGIMSGSL
                     PWVMIIVGVFMAIVLYALNLPIMTIAIGFYLPIATTSIILVGALIRLFVELVSKTEKE
                     KEVKVSNGISLSSGLVAGGSIIGLIGIILQVTGVVTPKVPSGFAATNSMAIALLVVLV
                     VLTALPIVLSKVKNNEQE"
     gene            complement(28641..30830)
                     /locus_tag="PC414_001965"
     CDS             complement(28641..30830)
                     /locus_tag="PC414_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HDF2464360.1"
                     /translation="MYEVNLERAMNYWMDLLDDYTNIAKIPNDFFYKDCSDEVVDEVF
                     KMNIEKHERLIQYSKVNNISIDSIIETIYGLILQKYIYEDDVVFGKTMNIIPIRVRVR
                     QSEKFIDVVRKLKRQWSRSLEYGYYLLSDIENKNHLNTKLINTIFIAKSFDLSYDYDI
                     EKKGYDLSVIVYTKRALKIKIKYKPSIYKRETIRRILNQFDYIIEQIIEDNDILIEDL
                     EFVCKEEEKVLLEDFNNNQYEVPYDKSYVDLFREQVKVTPSNIAARDENRKLTYKELD
                     EVTDKLAGYLNHIGVESEDIVAVMLPRDINIIITAIGIMKSGGAFFPIDTSNPEERLN
                     YLLEDSNAKVVITTDELKSKVVNENTIVLDINDEEMFKFGYELTEKITSSNCAYTIST
                     SGSTGRPKTIAIEHKSLVNMCYYSVKSISATENDICGIYLSFSFDAVMKQLFPYLLVG
                     ASIDIMPEEAKFDEYTVNEYCEENDITILALPTAFARLFIQNCNNNSLRVVQTGGERL
                     KGYRKRNYELYNEYGPTEFTVVSTSFHVDREYGKIPIGKPIFNTYAYVLDKKNKLCPI
                     GAPGELCLSGIQISRGYLNKKGLTEQVFVENPYKTCEHNKLMYRTGDLVRWLDDGNLD
                     YIGRMDNQVKIDEFRIELYEIENIINNITEIKSVVCISRTNDDGDIYICAYYVIDEED
                     SGKINERTIREYLNEHLPPYMIPTIIMRIDKIPVTPIGKVNKRALPE"
BASE COUNT        11665 a         4362 c         3946 g        11076 t
ORIGIN      
        1 gttaattatg aataaaataa ttatgccttt gaaaaatcat taaacttaaa attaattata
       61 aaaaaactct accttactaa ctatacagct cacaatctat ctatttatga aaaaatatga
      121 tataattgca ttcaaaaagg aaatctttca atatactaga atttaagcat taactaattt
      181 tatctaatat tattactaaa ctaaaaaata tttgtatttg tagcattgga gcaaataact
      241 taatgtatat tcattttaaa tacttattta aaatttagca tttaaattat ttacaaatta
      301 acatttaaat acttctagca taaatacagt tgtcatcaaa atttaaagta tcttatggag
      361 gaataccatg caaaatcata attatataaa aatgttaaat aaaaaggtag aagtattatc
      421 taaggaatta gatagtagaa attttaaatt aaaaacctta aatgaaatag attttttttc
      481 taaaatacca aacagagaat ttatgtacca acatattgat gactgtattt cgaaaaatat
      541 acatatagga atcatgttat taaatatcga taactttaaa ttatttaatg atatatatgg
      601 atacctagtc ggagaaaatt tattaattac tctaactcaa aacctattgc actttattcc
      661 agaatcaaag ggaatagtcg cttatttaaa aggagaaggt tttttaattg tacttccaaa
      721 tgtatcaaat aaagagttaa ataatatggg tgcaactatt atagagagcg cccgagaatt
      781 aaaaataaga cttcctaatg aaaaagaaat atactccaat gttacattta gtatcggcag
      841 cactatctgg aatggtgaac caaacatgac tactttgatg cttttcaacc aagtgtatag
      901 tgctttaaaa aaggctaaaa gtgaaggtaa aaatagatac attctatttg attttaaaga
      961 taatagtttt tacaataaat tagaaaatga taaggagttc ttatcagaaa tatcacatgt
     1021 tctacaaact aaacaaattg aagtatttta ccaaccactt tataatataa aaaataatac
     1081 tatagttgga tgtgaggcac ttgcaagatg gagacaccct attaaaggac ttcttactcc
     1141 agataaattt attcctttat ttgaacagtt tggtctgata acggaattag atttgcattt
     1201 atttgaagag aactgtaaaa atataagaag atggattgat aacaatgatg tttttgttcc
     1261 aatttgttgt aacttttctc gtcttcattt ttttaaccct aattttccaa gtatgctaaa
     1321 aggtattact gaaaagtaca gcgtctctac atcaaatttt agcattgaaa taacagaaaa
     1381 tatattaata gaaaatacaa atacaattat tcaacaacta gctgaacttc gttcttatgg
     1441 tttttctgta tcaatggacg attttggttc tggatattca tcttttggta tgttgcaaaa
     1501 tctacctttt gatataatca aaattgatag gatattttta tctcataatt taaatgattt
     1561 taaaaataca accattttgt actctataat aaacattgca agagctcttg ggatgcttac
     1621 agtatgtgaa ggtgttgaaa cagaacaaca tgtagaattt attaaaagta taaattgtga
     1681 tatcgcccaa ggttattatt atgcaaaacc catggagtgc aaagaatttg aaaaattact
     1741 atctaaagag aataattcag agaaaatgtt tataaaatat tctgatatag gaaaatcttt
     1801 aatagagaag atatttgatg gattttttat tatgcaagat atgaaaaaac taagagctaa
     1861 tgtcactgaa gacattcaat ggtttgattt ttacaacaaa actgaaatta catcttttcc
     1921 taaagtaata gagcattttg aaaaacatat taaaggtaaa aaattttcta ttctttacaa
     1981 aagtataacc ccacataata ctgaatcaga ttcattatta attagtggag aagccgtttt
     2041 atcagatgaa gcttctgaac atcaaaaata tagcaatttc tactttagtg caaattgtat
     2101 aatttcagaa aataaattac tcttagcaaa attacatttg gatttaatta aaactgctgg
     2161 ctatataaat gaatttgaat catattcaat acctaaaatg acaaatacac atcttgatga
     2221 aagtaatatt ttatcacaat tttattcaat gttgcctgta ggtataattc gctacgattt
     2281 atttggtgat atgattatta cttatatgaa tgaagaaatg tttgatatta taggttatac
     2341 aaaagaacag ttttttggtg agatgggcgg aaatttaaga gcaattgttc atccagatga
     2401 tttagacttt gtttataaaa aaagtttgga catgattgac acaaataaaa ttgaaccatt
     2461 tttatataga ttcataaaac gagatggtca aattgtcaca gttatgtata cacaatgtaa
     2521 cctttctgca attgatggaa ggtcgattgc ccagggtatg tatgtagatg taaataaaat
     2581 aaaaaattta acaaaaatct agtacaaagt attttatttt ggagaaatta atatgtttaa
     2641 agaaattttt ttaagaactt ttccaggatt tctaattata agagattcta actacagaat
     2701 tatatttata aatgataatt taaaaaattt aattaaaagt tatatgcaag ataatccact
     2761 tggaatgact aatatagaaa tagctaagaa actccctgat aatattgcta aattttttac
     2821 ggattctcac aatattcaat tagattggga aaaaaattat ccttatgata aaatttcaaa
     2881 ttggatttta gagtttaaaa aagatactac aagctattgg aatgttttag aatacaaagt
     2941 agatgttgat gaaaaaacct acataattac tatggccaat gatataacaa aactctatga
     3001 agaaaataag agaaatcttc actactcaat tactgaccca ttaactggag catataatag
     3061 aaaatattta aatgatagat ttgacatatt tataggagat tatattgtac taattgactt
     3121 agataatttc aaaatgataa atgactatga aggtcataat gtaggagaca agattttatg
     3181 tgattttgtt tcacttttaa ataaagaatt aataaattct acttctatta tacgattagg
     3241 aggagatgaa tttattgtta tattctctag tgatgttgac aaaaattatg tttattcaca
     3301 gctagaagcc ttaagagaaa attttttaaa agtattctct aagtataagt atctttcatt
     3361 tagttatggt gttgatacag taaaaagaaa tttaaaattg acaatagttg aattggataa
     3421 aaaaatgtat aaaaacaagg agaaaaacaa gaaaaaattt gataaaaatg attattaaca
     3481 ttgttatttt aaaataaatt aaaattatct ttcttttttt aaacttgtac attagtttat
     3541 aaatatgtat ctatacaaga tattgacatt aagtactgaa gcatatattg tttcagttct
     3601 tttttgttaa aattaactta tttaacaaag atatcatagt ttaacaaagc ttttaattta
     3661 aatatattga tgtataatat aattaaaata taatagcaaa aagaaaggag gaatatcatg
     3721 agcatactcc taaaaaaagc acccaaatta gcaaaacata ttataacaag tttttatata
     3781 aatcgagata tcaatgaagt attaaaatac ctttgtgaaa atgttacatg gattggacca
     3841 ggagaacagg aatttctcac ttcttttaat gaaataaaaa attattttta tgcaggtcaa
     3901 gatgaaattc cttcttgtga tataaataat gatatatttg aaactgtaag tgaagatgag
     3961 aatagatgta tggtattagg aagatatact gttcgaacta aagaaaatgc tcaaatgatt
     4021 ttagaggtaa atcaacgttg tacatttgag ataattgagg atagaggaaa attattaata
     4081 aagcacatgc acatatcaaa cccttatggt gaaatgcaac ttgatgaata ctttcccact
     4141 aaaattggaa ctcaaagtta tgattatttg caaagattac taaaagaaaa aacagaagtt
     4201 attgaaatga ttacaaataa tatcaatggt ggcttaaaag gtagcaatga tgatagtaca
     4261 tactcatttt tctatgtcaa tgagggattg cccaaaatat taggatacac atatgatgag
     4321 ttcatggaaa tgagtggtgg ttcagcagtg ggtgctgtat atccaccaga cttacctaaa
     4381 gcactagaag attgtgaacg atgttttgca aaaggaccta catattcatc tgaatataga
     4441 atccgaaaaa aagatggaac attaatgtgg gtattagatt ctggaatgaa atcgttaaat
     4501 agtgatggca tagtaaaaat taatagtatt attactgaca taacccaact aaaaaacata
     4561 gaatctgaac taaaattaga aagagagcga tatcgtattg ctttgcaaaa tattacagat
     4621 atcatgtttg agtatgatat tgaaaacgat acccttataa aatatcaacg tgtagaaatt
     4681 gacaaaaaaa ttgaattaga aaattttgaa acaaaaaatt actccaaact attagaatct
     4741 ggtaaaatta ttcaccttga tgatattgga aaactactag aagtacttca tggaaatctt
     4801 cgtgaaacaa ttgagataag acaagtaaat tcattaacta aaaatgaatg gagatggatt
     4861 cgtgttcaat gttcagttat atatgatagt gaccataatc ctatcaaaac aataggcgtt
     4921 ttaaaagaca taactgaaga taaatctaaa ttagaaagtt taattaatca ggctcaacgt
     4981 gacccactaa cacaattata taatcaaaga gtatcacaaa atcttattga agagtatcta
     5041 tgtagttctg attccaaaaa taatgatgct ctattagtta ttgatattga tgattttaaa
     5101 actgtaaatg atacttttgg gcatctagag ggaaatgaag ttttagttgc tgtatcaaaa
     5161 atactattac ataacacaca tgataaagac atagttgctc gaattggtgg agatgaattt
     5221 acaatattta taaaatcatt aacaaaggat ttaataataa aaattactaa tgatatttta
     5281 aatgatgcga gtaaaataaa agtaaaggac aatcataaaa taacattaag tattggtatt
     5341 gccttcactg atgattcaac taaattatat aaagatttat tttctaaagc tgataaagct
     5401 ctttatctat caaaagcaga tggcaaaaat tgttatagta catacgaata ataaagctat
     5461 ttatacgacc aagaatgaac ctttaaattt aatttcatag tctaacaatc aaagagttga
     5521 agctaatctt atagctagtc aactagttcc tagaaaaaat ttaaacaata tatttattga
     5581 tactgctcat ttttctaaag agcaaattta tataaaaaat taaactttta tatacataca
     5641 gatactaatg ataaacagtt atcattagta tcttctatct taaaacaaaa tgataattta
     5701 tctatataaa aaagtgcttt gttgacagag ttcacttttt tatataggta cagaatttaa
     5761 gtataaatac atgttttatt ctatacattt tagttaatgt aaaatttctt gattgtactc
     5821 tctaactttt caacattata ctatttatat accacttagt taatataaaa ctataaaagt
     5881 gttgcaatta ctgggtttta taaagttaat ttacatacca cttagttaat ataaaacttt
     5941 agcaccttat ttatctatag agctacagaa agtatttaca taccacttag ttaatataaa
     6001 acatgtattt atttgttctt gtactattgt tttactctat ttacatacca cttagttaat
     6061 ataaaacaat atcaaaacaa ataaaaattt ttaataaatc attatttaca taccacttag
     6121 ctaatataaa acattctggt actgttgcta tttatgttcc gtattcagaa tttacatacc
     6181 acttagttaa tataaaactt tggagttgtt acagattatt tgccttatct ctctatttac
     6241 ataccactta gttaatataa aacccccaaa taaactgggc attttcaata cttacacata
     6301 tgcaactctc ttaaatttgc agtgaaccat gagtagtgca aatgataaag cttatcatat
     6361 accctcaaca ccttatattt caagcgttaa gctctacttt gtaacaaata tcgctcactg
     6421 caaaactatt tacattttta ttatatcata aatatattat ttttgaatat ctataccaat
     6481 ttgtggtata ataaaagcaa gaagaactac aatctattta gacgtagagt gaagttcaaa
     6541 tactaatgtt tattcttttt gaacttgatt tttagttcaa aactaaagtc actcctgcct
     6601 gtgtgaacta tacccgctgt tttatacagt taaaacatgt aaaaatagtt ataaagtact
     6661 agtgtaactc attagtactc tttttattat attcatataa ttaatatcta tattttaaag
     6721 cttactatct aaaaaagagc acgtcaaata aaaccctaga tatttaattt ttttagagat
     6781 acttaaaata aattatgctc tctctgtact taaatatttc ctaaattcaa atggagacat
     6841 agaatttaat ctattttgat atctaaaatt attatagtag tatatataat catctattgc
     6901 tttaactaat tattcatatg tatcaaattt ccttaaatag tacatttcta cctttaaaat
     6961 tccccaaaat ccttccatag gagcattgtc aaggcaacga ccaactcttg tataatatat
     7021 gtatatctaa caccattaag aaatatatat tttataatat atatttcttc atttaatgca
     7081 cttagaaaag caaagaaaat acagattagt acacttttta gagaaaaata aaattaatac
     7141 atctaaaata ttaaaaaata ctttagataa tctaaagaca catctcaaag tttttaacta
     7201 aattataaaa ctaattacaa atgctatgta acgataataa ataaaaaatt tacttcgctt
     7261 aagcgacgtg tcgttgaaat atttcatgtt gccaactgat tcataagtcg ataagaaaag
     7321 aacactaatt atttagagtt cttttcttag ctacaagtgg attataatac ttttaaatct
     7381 gtgagaaaat catatataat aatgatatga acaagtaaag aaaatacaaa aatgacaaaa
     7441 ttataaataa aatattataa aatcataagg tcataattgt tatattgtag aaacaaagta
     7501 aagagagagg gtggagaata aatgaatcaa aaaaaaataa agattagtat taaaatggta
     7561 attataggta tattttctgt aattattgct tttgtattat ccagattatt cgttgagtat
     7621 attgatgaat tattaaggtc taattggcaa tatcagctat ttgaaagtga gagttcatat
     7681 ataatatttg ccaattgtat tatgttctcg aatatattat tagaaatatt tttaatatat
     7741 atttgtagaa aatttagaaa aatctaatat ggtcaacgta tttcgaatcg aatacttcat
     7801 gattatttag tctattgtgc tagttaaagt ttaattccca tataaagtaa attgatttta
     7861 gtaaaacaaa tcacccctaa gggttagttt atggataagt catccaaata ttaactcttt
     7921 aatttttgct atagattcat ctttgctcat aactttactt ataaaaaatg aaatattata
     7981 agctagaact tttattctaa ttctagtcat aaatcctagc atagagttac tttttttata
     8041 ttataaataa aactatacaa caaagataat atttagtaaa aataactttc attttaacta
     8101 aacttaaaat tcaattactc tctaaattga cgtttaggaa ttagttcaaa ataaaaaaga
     8161 gtgtatatat tgaaattact acatttcaat atatatactc tttttaaatt tctcagtttt
     8221 ccatggaact ttactttatt tatttctgta ttagttgtat tttttatgca tttttcttta
     8281 aaatcatatt ttctgattca actcttatat tataaaattt ctttactaaa ttattctctt
     8341 tttctatatc ttcatatata ttttttaatc gccttttacc taatctttta tctaacatta
     8401 ctaatgattt aactaatata ttatatgatt ttatcgcatc ttctattgag atatttaaac
     8461 atatatttac tgcttcataa aaatcaaatt cattaaaaaa tccattttta tttacaatct
     8521 tatatccctt tgcatctctt tctaaatact cttcctaaat actcttcaaa actttcatca
     8581 tctaatctaa atcctatatc actatgaaaa ataggttttg catttggata tagttctatt
     8641 gttttatcaa aagtttcgaa taccaagtta ttattattcg attttccaat agtccactat
     8701 aaacaataat aagtttgttg ctccaaataa tgtaacactt gtagaaagat gttacagata
     8761 tataaaagat agagaatgta gtttttctcc taatactatt agaaatagaa aaaatatgat
     8821 taaaaatcaa attgaagttt tctttaaaga tatgaaatta atagatataa caccttctat
     8881 tttacagtct tatataaata acatatacaa tgagcacatg ttaaattcaa taaaaaacca
     8941 agtcgatttt ataaaatcag tattgaaaga atcttataga ttaaaagaaa tatcagaaaa
     9001 tatttgtgat tttgttacaa ccccaatcaa aaaagtagtt caacttctaa gctttataca
     9061 aaacaaaaag ctcaattatt attagaaaaa tcaaaaaata ttcctatagg tataccaatt
     9121 tttttgatgc taactcttgg attacgtttt ggtgaagctg tatcacttat atggtcagat
     9181 gtagaccttg ataaaaaatc tcacatgtaa atcaaacatt agtttatgta tacaataaaa
     9241 taatcttcaa agaccctaaa acccctatat caaaaagaaa attatttgct cctgatgaac
     9301 ttataagttt gcttaaaggg gaaaaattaa aacaaaataa gttaaaatta cagggtatat
     9361 tgaaaaatga atttgatttg atatgcttaa ataaaagatt caacccgtga gttcaaccaa
     9421 cttttgttgt tcattttaaa aaactattaa gagataataa tcctagagat ataaaagtac
     9481 atgaattaat acatgcaaat gcaactttga tattactttc aggtactaac ataaaagcta
     9541 tttataaaat attagtacat gcagatataa aaatagctat gaatatatat tccatattct
     9601 cacttattag atgaaatgaa taaagaaaca tctgaaaata taagcaaaat actattcaaa
     9661 taatgttcag ttttatgtca attaaatgtc actcaaagtg cttttttttc atttgtcagt
     9721 taaatgtcac ttaaagtacc tttttcctat ttgtcagtta aatgtcagta atatataaat
     9781 aagatatatt tataagaccc ttaaaatcac tattatttaa taaaatttat aattatatac
     9841 tatttgctgt tgacattggt attataccat agtttatcat ttatattaga aataaaatta
     9901 ttaattaata gtttcgtaat tttataatag atttacaggg agtgatagta aatggataca
     9961 aaaatatcta taaacgaaaa catgagtcta ggtaaaagat tttttaaata cctagcacca
    10021 tctgtagttg caatgtgggt tttctcacta tatacaatgg ttgatggaat atttgtaagt
    10081 aaaggagttg gagaattagc tttagctgct gtcaatatat caatgccttt tattaatttt
    10141 atatttgcag tttctctatt attttcaact ggtgcatcta ccataattgc aatctatctg
    10201 gggaaaaaag atattaaatc tgctaacgaa gtcttttcat ttaacttagt atcaataatt
    10261 atattatcaa taattatatt agctataact ttttttaatt tagatagatt agcactattt
    10321 ttaggagcta cagaatctac aattggtatg gttaaagatt atttaggaat aataatattt
    10381 tttaacggat tctttatagt gtcttattct ttagaagtta taataaaaac tgatggcttt
    10441 ccaatattag ctactgttgg tgtaataata tctgctctta caaacatcat acttgattac
    10501 ttgtttgtaa ttgaatttgg atggggtgta aaaggtgctg gtattgcaac tggtttgtct
    10561 caagtctttt ctactatttt cttcttaata cattttttaa gaaaaaactc tactttgaat
    10621 ttttcaaaat ttagaattga ttttaagaca cttagaaaaa ttgtatttat aggttttcct
    10681 gattcaacta ctgaattaag ttgtggtata gttgttttac tttttaactt aagtcttact
    10741 aaatatattg gagaaaatgc tcttatttat tatagtgtca taaattatat aaatacatta
    10801 gtacttatga ctatgatggg tataactcaa ggtatgcaac cacttactag cttttattat
    10861 ggtgcaggaa atatagataa tgtaaaaaaa ttattaaaaa tggggataaa agccacaata
    10921 atagcatcag ttgctgtatt tgcaatttgt atggcttttt caggaccaat agtctcttta
    10981 tttatacatc cagaagaaac aatgctattc aatgaaggag ttcgtgtatt taaaatattc
    11041 tctatatctt tcttattggt aggaattaat gtaataattt ctggattctt tgtatcagta
    11101 gaaaaaccat caatttctac tgttatatct cttggtagag ggcttgtaat agttgtttta
    11161 agtctaattt caatgatact aatatttggt ggacaaggaa tatggatgac tactattgtt
    11221 tctgagttta tatgtcttat tttatcatta gtttttctaa aaaagaattt ttctacatta
    11281 gactctaatc taaataaggt tgcttagatg ataagagtga caatatatga aaatatctaa
    11341 ataaaaaata cctattatac aatcttgcaa atattaaacc tttgcaaaaa ttaggattat
    11401 ataataggta ttttatttat gtaattattt tatttctaaa tatccatagc catcttttat
    11461 taaagttcct tttacttcat attctacagg ttttttaaat attggaccat ctacgacaaa
    11521 tgtatgatac tctccatgtt ctccacatgg gtctgcacca gcatttttta tcttctccac
    11581 aagttctcta gttaattttt ttcctaaaaa ttcctctcct aagtgcttta agtttacttt
    11641 tgttacaaca ctacaaaatc cactgtctat aaattcatat actaactcct ctctactctc
    11701 ttgccataat ggtaaatcta ctttcatacc aactgcttca catctatcag tatcccattt
    11761 tctatgactt tctatatcta tatctccata tgcacatata gttgctccca tgtttttagc
    11821 ttttattaaa gccctctcaa agctctcttc atatccttct acaccacact cagcaactaa
    11881 taaaggtaat cctatattgc tacttacttg cttaagcata ttatattcta aattatgtgt
    11941 ccatgacttt ccatgttctt cacttatagt tgtcaagagt gccacaggct tatatccttt
    12001 tttcaacatt cgatttaatg ctaatatact atccttacca ccactaaatg acattacaaa
    12061 tttttcatca gccaatttta acacctcttt catctaactt tgttttatta acataattat
    12121 accaaatttt tgagttgcat agcaaattat attaaattgt taattcacta taaagaatgt
    12181 ccctaatatt aaaagtataa cccctatgta tttgtataaa tgcattgttt cttgtcccaa
    12241 aattacgcta tcaataaaaa atccaactaa aacttgggat acaagagtta atataagtgt
    12301 atttgatact cctaaatttg gaattgcctt tactgtaaaa tatattatta gacctccaaa
    12361 tactcctcct aataacaact tataattaaa accatttata gatactagac actttaagtc
    12421 acctgtaact attatacttg ccaaaaaaaa tattgctcct acagttaagc ttataaaggt
    12481 ggccactaat gctgttgtac tttttcctag ctctccattt ataaaagctt caagagctgt
    12541 agaaccacct gctaataatg caaatattat tgcccataat tttgtcatgg acaaacctcc
    12601 ttctatataa ttctattaat agaagtttat gatacaacct attaattaat tactaagtat
    12661 agtttaattt ttatacacta ggctttattt ggatatgaat ttctgttata attgttctat
    12721 cgattttgga ggtgatttta tgtacacaaa ttttaatatc gattttaata actttaataa
    12781 aaaagaaaat gccattaaat ttatgttaat gggagtatta ctcattatat taggattatt
    12841 atgccttaca tttaagacat taggtataaa attaatttct tggacttttg gtattgcact
    12901 tttattcttt gcatatttaa atttaaaaaa tattaatgaa ctaaaaagat atgctactaa
    12961 agaagaaatt aaaccctcta ttaatatcca atgggttcta ataattgcat gtatattact
    13021 ttttgtattt cctcaaaaaa tacaaagcat attttcatta tttttaggat tttatttaat
    13081 ctttaatcaa ctagtagcac ttgtaaatag taagaataat ccttattcta aatttacaac
    13141 ttggaatata gttaagattc tatttggtat atgccttata ttatctccac tttttttatc
    13201 tagatttata gtatcgataa tgtctttttt cataatatta tttggactag ttctattttt
    13261 ctctggaaat acagcgagaa aatattaatt attttctcgc tgtattttta tattacttaa
    13321 aatagaattt gtttaagtta tctatttgtg gtaatatgtt ttttgtacaa gctcagcgta
    13381 caattttatt agaaataggt gattttttgg ataaatttaa atatgctttt gataataaaa
    13441 gatatcatac ttggaattac tatcttagaa atacttttgg agaaaaagtt tttaaggtat
    13501 ctataaatgc aggatttact tgcccaaaca ttgatggaag tcttggttat ggcggatgta
    13561 cctattgtag caaagaaggc tcaggtgatt ttgctggaaa ccctaaagat aatcttatca
    13621 gtcaatttta taatataaaa gaaatgatgt taaaaaaatg gcctcatgca aagtatattg
    13681 gctactttca agcctataca aatacgtatg ctcctcttga agttttaaaa gaaaaatatg
    13741 agactatatt agaacttgaa gatgtcatag gtctttcaat atccacaaga cctgactgtt
    13801 tgccagatga tgttgtagaa tacttgtctg aactaaataa aagaaccaat ttatgggttg
    13861 aacttggact tcaaacaatt catgattcta catctaaaat tataaataga ggtcatgatt
    13921 ataaaacatt tgttgaaggt gttgaaaaat taaagtctaa aaatataaag gttgtcgtac
    13981 acattataaa tggtcttcct ggtgaagatt ataatatgat gatggaaaca gcaaaggcag
    14041 ttggtaaaat gggtgtagac ggtataaaaa tccatctatt gcatgttata aaagatactc
    14101 ctatggaaaa gatgttacaa aatggtatgt taactcttat ggaacaagat gaatatatca
    14161 atcttgtctg tgaccaatta gaaatacttc ctgaaactat gataatccat agactcactg
    14221 gtgatggtaa gagggatgaa ctagtaggtc ctatatggag cctaaaaaaa tgggaaattt
    14281 taaataaaat tgatgatact ttaaaggcta gaaattctta tcagggctgt aagtttgttt
    14341 aatacaaaat aataactata gaaatggtta tacaaatata gtcctttcta tagttattta
    14401 gtatttacaa aacaaaatat taatttgtac atttatacaa tatataataa aaaatagata
    14461 gtctaaactg tatctactta tattttattt gtactgtcca ctttattcta taaaatttat
    14521 aaatagttat tttttaatac caaatataat ttggtataat ttttgttgga atctacaatg
    14581 tataccaata aatccttttt aagtatataa ttctaaatac tttcagtttt ttcaatctgc
    14641 tttaacttgt tggacttata aaatattctt atttcatcat aagaaagatt aagtgctact
    14701 actgcagata ttaacatcaa catcactcca aataacttcc ctaaggaaaa actttctcct
    14761 aatatagtgc aaccaattat tactgaacca actaattcta ctgatgccac aacacctgca
    14821 actgacaact ctacaccttt agatatacct gctgcataaa aaatatatgc aagtgcagct
    14881 ggaacaatgc ctaacattag catacaagat aaaacatcta aatccttagc ataatttaag
    14941 atttcccaag gtcttgaaga aggaatcata aatatagctc caaataagaa actgtatact
    15001 aatattgttg aacttgaaaa ttcctttaaa gcatttttac taatagttgg cataagtgca
    15061 tatgctattg ctgataatac tccaagtaaa agcccaaaag cgtttaaacc ttgaaaatcc
    15121 aaacgtccac cagtcactgc cataattgca ccaataaaac aaagaataag tgaaaataat
    15181 ttgcttctag taatattttc tttataacat aattttgaaa atattgctaa aaacagtggt
    15241 gatgtatata gtaaaactgc tgcaattgat acacccgcaa tgtcaattgc tttaaaataa
    15301 cataaattaa acattgcctg acatataatt cctattatta ctgaatatat aattcctttt
    15361 ttgtttatct ttaagatttg tggtgtcctt atagatgaat atacgcctaa aactataaat
    15421 ccaaaaaaca atcttgtaaa tgctacttgt tctgatgtca gtccatttcc cattaaaaac
    15481 tttccaaata atccaagtgt agcccataat aaccctgcta ttgctataaa tacataccct
    15541 ttactttttt ctcccatttt aaattccctt ctatttttat ttagtcaatt tacactcttc
    15601 tttttaagca aaaactatgc catcttaaat caatatatat cgactctaaa accctagtct
    15661 ttccaataat actaataaat tttcaatttt tttatattta tttttcgctt actttaagtt
    15721 gatttagact tattttaatt gtaaaatgag ttatatataa cttatttaaa gttaaaatag
    15781 actgaaactt atatataaat atcaaatttt aagtatatta atcaattaaa cctattttaa
    15841 tatatcatta aaaatttagg aggaaaatat aaaaatgaat ttagatttgg aattttatca
    15901 aaaaatatta gaagcttccc atgatgaaat atgtgtttct gatgataaag gaataataat
    15961 atattgtaat aaagcatttg aagaaaatta tggtctaaaa aaagaagata ttttgggaaa
    16021 aaatgtttct tttttagaag actctggata ttcaacaaaa agccccatac caattgtatt
    16081 aaaaacaaag actaaatttt cactggaaca agatactcaa acagggaaaa agcttatcat
    16141 aactgctacc cctatatttg atgaaaatgg acacttagaa tttactgttg aaaattgtag
    16201 agatataact gaacttaata atataaaaaa taaacttgaa gacacaaaaa agcaagtaaa
    16261 aaaatataaa tctgaagtag aaacactata cagaactgca cttagaattg aagatactgt
    16321 aattatggac ggaatagtta tgagaccaat cataaacaca gtaaatcatg tttctaaaac
    16381 ggatgtaagt gtacttttat tgggtgaatc tggaacagga aaaagctcac ttgctagata
    16441 tatacaccac aacagcaata gatctaatgg cccatttata acaataaatt gtgcaactat
    16501 atctccacaa cttcttgaat ctgaactttt tggatatact tcaggtgctt ttacaggtgc
    16561 aagtactaaa ggtaaagttg ggctcgtaga attagctaat ggaggaactc tttttttaga
    16621 tgaaatagga gatataccac aaaatctaca ggcaaaattt ttgcaattaa tacaggatag
    16681 aacttttact ccagttggga gcttaaaaaa taaaaaggtc gatataagaa ttatatctgc
    16741 tacaaatgtt gatttagttt caaaagtaaa agaaaaaaag tttagagaag atttatacta
    16801 tagattaaat gttatagaaa taaaattgcc tcctcttaga gaaagaaggg ataatctagt
    16861 ggaaattata aaatattatt ttaataggta ctcaagtgac ttcaacctaa ataaaactat
    16921 ttctaaggaa gctatggatg ctattgcaaa ttatagattc ccaggcaata taagagaatt
    16981 acaaaatata attcaaaaaa tcttgcttac ttgtacagat aatcatataa caatagatga
    17041 cttaccaaac attcttacaa aaaatataca tattaccaat aatggaaata aaacacatat
    17101 atcccaaatt aataaggtta ttatatctga ctcaaaatct actaattata aaaataaaaa
    17161 ttttgacact ttaattaaag agtatgaaaa aaatataatt ttggatgctt atgaaaaatt
    17221 tggtagttct tataaagtag ccaaacacct tgaaatctca cagtctaaag ccaataggtt
    17281 aattagaaag tatactaata cttaatttta aatttgataa ataaaaactt aacactattt
    17341 acatattgta aatttggcca tttactgtta ttatgaatat ggttgaaatt atttaaataa
    17401 atactgttga cttagtttca tatatttacc attaaacttt tatcataaat atcatatcat
    17461 aataatatat ttaataagtc taataaacta tacgaggtgt aaatatgtta atgaataccc
    17521 attttattat ggcaaaatca atacttgata atatagatga aaataaaaca ttctttataa
    17581 gcgaaaaaaa ttttatatat ggcaatataa agcctgatgc tttttctaaa tataaattaa
    17641 aaaaacatta tttagatgag tcatttaata tgattgtttc taaaataaac tatctatgca
    17701 gtcttaatat agatagtctt tctaaatact tttcagtaag tagacttagt caggaactag
    17761 gagtcatatg tcatttttta tgtgattttt tctgtgtagc tcacagtgaa cgttgggaat
    17821 ttaagcatag cttaaataaa catgttactt atgaaagaga acttggagca tttgcaaaag
    17881 atgttgattt atctaaaatt aaatgtggta ctataaatag tagttttgag aagtttttta
    17941 ctaaacttta tagtgattac aaaaagaaaa ctgattatat gaatgattta aaattttcag
    18001 cttatacttg taatagtgtc ataaattata tacttgactc tattttatct aatacaataa
    18061 aatcatatca tatagcaaat tgcggttaac ttaagaattt ttaaacttat ataaacttat
    18121 aattgaatat aattaaaatg actgctctat agaatataat tttatacttc tctaggacag
    18181 tcatttttta tactaactat ttatctccat tataacatct atggcacttt ttacactatc
    18241 tactacataa tttgcacttt gctttacatc ttcccttgcc ttagacattg caaaactata
    18301 tttaaatcct tctaacatgc aaatatcatt tggagaatcc ccaatagtag caacttcatc
    18361 tggacttaca tttaacgttt ttattacatg ctctatactt cctctttttg acacactttt
    18421 aggaactata tttaaagtat atgttccaga aaaatatatt tctaagactt cactaaactc
    18481 actttttaga taaagttcta ttctggataa ctcttccaaa ttttcagaac atacgcctat
    18541 atttcctata agattatcaa aactacttct tttttcaaaa tctgggtcaa ctataaagtc
    18601 actagaataa tgtctaaaag cagtggtatc actacccaca attacatcac ccttatattt
    18661 aaaataaagt tgtgcatcac ttgactcaaa tctatcaatg acttttttta ctaaattatc
    18721 atctatcgtt cttttaaaaa ttacattata atctttatca tatataaatg aaccattttc
    18781 acatacatag tatccattca tatctaaact atctttgata atattatgtg cttgtttaaa
    18841 aattctacct gtagcaacat taaactgtac tccctttttt tctatttctt caattgctac
    18901 tatatcttct tttgttatgg ttccattttc atatagagtt ccatctaaat cacaaaatat
    18961 atgttttatc ataaattaaa agcctccaaa aacaatttta tattttacta atactttaat
    19021 caaaataact taaagctttt ctaattgtat tctcaattca ttaagcaaat ctctagtagc
    19081 tcttaatctt atctttcttc tatccccatt aaatatattt ttctttacta ttgtttttcc
    19141 atttatatat attgcagtat aaaccagtcc cacaggcttc tctttggttc ctccaccagg
    19201 tccagctact ccagttgtag atatacctat attagcttta agtcctttag caatgccctc
    19261 agccatttct atagctgttt gctcacttac tgctccaaac tcttccaaag tttctctttt
    19321 tactcctaat cttttcattt tagaatcatt agaatatgtt acacaacctt caagaaatac
    19381 agaggatata cctggatagt ttataagaga tgaagaaacc aaacctccag tacaagattc
    19441 agccactgct attgttagat ttttttctac aagtagctta gaaacagcat cttctaaagc
    19501 agtctcccct tcagatatgg aaatatctcc ttcagtatat acatatttac caactctact
    19561 ttttattttt tcttctactg gttttattaa tttaaaagct tctctttcat tttcagcctt
    19621 tgcagttatt cttattgtaa cttctaattc ttttgcatat aaagcaactg ttggattagt
    19681 ctgctcatta attatatcga gaatttctaa ttctagattt gactcaccta ttccatataa
    19741 tcttaaagtc ttagatacta gcatttcatt tgtgaattgt tgtaagtaag gtttgacact
    19801 ctcattaaac atagctttca tttctcttgg aggtccaggc aatactatta taaatttacc
    19861 atcttttttt aaaattgccc ctggagctgt accattatta ttttttagta ctatagcatc
    19921 ttttggaaag tacacttgtt tcatattatt ttcggccatt tttttccctg ttttaacaaa
    19981 gaaagattcc aaaacctcta gtgaaggttt atgtagttct aatttctgac caaagtattc
    20041 tgcagcagtt tcttttgtca tatcatcacc agttggccca agaccacctg tagtaattac
    20101 aaaatcactt ctctttaagc tttcatcaaa acattctaaa agtctttgtt tgttatctcc
    20161 aacagtactc tgatggtata cttctattcc caatgatgca agttctttag ctaaaaattg
    20221 agaatttgta tttactatat ctcccaataa tatttctgtt cctacactta ttatttcagc
    20281 tttcaaaatt tatcactcct tgatttatag ccattaagac tagtcttaag ttcataacaa
    20341 taattttagc aaaaataata acataaataa agttaaaaaa tcatttaaac atatctttaa
    20401 actagattat tattctaaaa ccacacataa tataaacttc gtatatagtg gtactttcat
    20461 cctcaactgt taattcatat aattaagtat gtaaataaaa agtttacttt aaaataataa
    20521 aaattaaaat aggagtgtat aatatgaaaa tagcagttat aatgggtgga atatcatcag
    20581 agagagaagt ttctttaaat tcaggaaaag agatatataa taaccttgat aaaaataaat
    20641 atgaagttgt aaaggtaata atagatgata aaaaagatat atttacaaag ataccagaag
    20701 atatagattt tgcaatactt gctttacatg gaaaatttgg tgaagatgga tgtatacaat
    20761 caatacttga gactatggat ataccatact ctggatgtgg acctctttgc agtgggatgt
    20821 gtatggataa aaatataact aaaaaaatgc ttagagattc aaacctacct actgctccat
    20881 gggttttagt aaaatcagtt gatgaaatag actatgatga aatagataat ataggttatc
    20941 cagtatttat aaaaccaaac tcaggaggtt caagtgtagc cacattcttt attcattcta
    21001 aggatgaagt tgaagaagca gttagaaaag gtctggaagt tgatgaattt gtaatgattg
    21061 aaaaatatat accaggtgga gaatatactt cattcatatt aaatggagaa gtgttcccta
    21121 caatatctat aaaatctgat tcaggattct ttgattatga agctaaatac tcagttgaaa
    21181 aaggtgcaaa ggaagaagtt gtttacttag atgaagaact tcaaaaaaga gtaaacgaaa
    21241 tttctgagac ttgttggaaa atttttaatt gcaaagcata tgtaagagtt gatatgatta
    21301 taagtgaagg tattccatat gttcttgaac taaatactct tcctggaatg actcaaacta
    21361 gtttaattcc tagaagtgct gctgctagag gtattaagta cagtgaacta ttagataaac
    21421 ttatagaata ctctttaaat tagatgaaat aatttgaggt ggtaatttga tattttcaaa
    21481 tctaaaatta aatgataatg aaccaattta tatacaacta aaaaattata taagtgatat
    21541 gatatcaaaa ggactaattc cagacaatag taagctccct tctacaaggg agcttagtca
    21601 gcttttacaa gttagccgaa attctgtagt tttaacttat gaagaattaa aatcagaagg
    21661 attaatctac tctatttcag gaaaaggtac atttgttaaa tcaaagaata agtcttcaaa
    21721 tactacatgg tcattaaact gggattgttt agagaataca tactctaaaa aagccaatga
    21781 attggacatt ataaaaagtg aaattccttg gagctctgac ctgatttcct ttaaaagtat
    21841 ttctccagat ggagatttat tcgacatgga agaattaaaa aaatcctttt taaatagaat
    21901 ttcactagaa ggacataaat tactcaacta tggatatgca caaggttata agccacttat
    21961 tgactattta ctagaatata tgacaaacaa aggtgctgat ataagtaata aagatatact
    22021 aataacaaat ggattcacag aaggctttga tattattatg tcatctttta cccaagaaaa
    22081 agactacata atatgtgaaa atcctactca taatactgct ataaaaatta tgaaatctca
    22141 taacctcaat atcttaggtg tagatataaa tgaagatggt ttggacttca atatgctaga
    22201 agacaattta gttaagtata aaaataaaat aaagtttgca tacattacac cctcttatca
    22261 caatcctaca ggaattgtaa tgactcctga aaataggtac aaattctata atcttatgaa
    22321 aaaatacaat atagcaatta tagaagatgg atttaatgaa gaattactct acaatagttc
    22381 tcatatattt cctatctgtt ccctagataa tatgaataat ggggtcgtat atattggtag
    22441 tttttcaaaa attttatttc ctggaatgag aattggatgg atatttgcag ataaaaaagt
    22501 aatcaataag ctagaaagtg taaaaaggtg cagaaacata catgtatcct ttttagacca
    22561 aggaatttta tttgattatt taagtaatgg aggttttgaa aaatacatta aaaaaatacg
    22621 taaattctat ggagacaaat ttaactttgc atataaatgt ataaaaaaat atataaaaac
    22681 agaatatatc ttgggtgatg gtggtttgca tatatttata aaactaaaaa atatagatac
    22741 tagagtttta ttagaaaaat gttatgaaaa aaatgttata tttatgcctg gagacttatt
    22801 ttttacagat aactctggtt ttgatacttt aaggctcgga ttttctaggt tgtcttttga
    22861 agacattgaa attggaataa aaataattgg agaaactata aatgaaatgt tctaggttaa
    22921 ctaaatattg ttagcctagt ttatttttat gaggtataat atatatagtt tatatgaatc
    22981 taatactggg ggataatatg agtaaaaagt caaaacgaag aaaaattaat aaattaaaat
    23041 taacgattgt aataactgta ttatttcttg ctttattatc tatatatgag atactgttct
    23101 ctaatcccaa caaaaaaggt aatataaaga gtcaagaaac tatgcaaaat aaaaataata
    23161 caacagatga ttctaaaaat aatcaaagct ctaccaaaaa gcaggaagat aataatatca
    23221 aacctacttt ttcatattct tcaataccag ataatataaa aaataaaatg attggtaaat
    23281 ctatgcctac tgatgaacct ataagctttg atagtctatc ttatttaaaa cttacatatt
    23341 atggatttga tgaaaaaact catcaaggag aaatgatagt aaacagtgaa ctagctccag
    23401 aagtagtgga tatttttaag gaattatacg aaaaaaaata tccaattgaa aaaattaagt
    23461 taatagatga ctatgatgct gtagatgaaa aatctatgtc tgataataat acctcatcat
    23521 tttgttatag aactatagct ggtacaaatg tagtttcaaa tcatggtaaa ggacgtgcta
    23581 tagatataaa cccattacag aatcctcaag tttctggaaa tgatgttaca cctaaagtaa
    23641 gtacagttta tgctgatagg tctagcacaa aatttggaat gataaaaaaa ggtgatgatt
    23701 gttataatgc ttttgtaagc agaggttgga gttggggtgg ctattggaaa aacccagatt
    23761 atcaacattt tgaaaaataa tagattagaa ataaaggtga attatatgaa tttaatgaca
    23821 ttggaaaata taagtaaaag ctattctgaa aaaaaattac tagaaaatat atctcttgga
    23881 ataaatgaag gggaaaaaat aggatttatt ggtgtaaatg gtactggtaa atctacctta
    23941 ctcaaaatta ttgcaggagt tgaagaaagt gagactggta atataacaaa aactaatggt
    24001 attaggatag aatatctacc tcaaaatcca acttacaatg aaaattcaac tgtacttgaa
    24061 caagtattta atggaacttc tgaagaatta aagctacttg gtgaatatca agaaatccta
    24121 gaaaaactta attcaaactt tacagaggaa ttaaataaaa aattattatc agtacacgaa
    24181 aaaatagatg ccttaaatct ttgggattta gaaagtgaag ctaagtcagt acttacaaag
    24241 cttggtataa atgactttaa tcaaaaaata gcagaacttt ctggtgggca aagaaagaga
    24301 gtctctctgg catcagccct tattacacct tgtgagtttt tgatactaga tgagcctaca
    24361 aaccatctag acaatgatac tatagattgg cttgaggaat atcttaattc cagaaaaggt
    24421 tcattgctta tgataactca tgatagatac tttttagacc gtgttacaaa tagaattatt
    24481 gaactggaca aaggtagatt attcagttat gatgggaatt attcaatatt tttggaaaag
    24541 aaaatggaaa gactttcgct agaagcaagt attgaagata aaagacaaaa tttaataaga
    24601 acagagcttg cgtgggtaag acgaggagct caggctcgta gcactaaaca aaaagctcgt
    24661 ttacaaagat ttgatgaact tataaataga gattctttta aacctgatga aaatctcgat
    24721 atatctgttg catcaagtag acttggaaat aaaataatag aaatacacaa tatatctaaa
    24781 tcatttgaag aaaatactgt tatagataat ttagaataca ctttaactcg tacagataga
    24841 attggtataa taggcaaaaa tggtatgggt aaatctaccc ttataaatat acttaatgga
    24901 gaaatagagc ctgatagtgg atatatttca attggagaaa cagttaaaat aggttgtttt
    24961 tctcaagacg actctcatat gaatattgat atgagggcta tagactatgt aaaagaagct
    25021 agtgactata tagaaacttc agatggtact aggattacag cttctcaaat gtgtgaaaga
    25081 ttccttttta atggtactat gcaatatact ttgataggta aattatctgg tggtgaaaga
    25141 agaagactac atcttcttaa aatccttatg acagctccta atgtgcttct tttagatgaa
    25201 cctacaaatg atttagatat tgaaacgctt aaaatattag aagaatacct agacgaattt
    25261 aaaggtgtag tagttacagt atcacatgat agatatttct tagatagaat ttgtaataaa
    25321 atatttgcat atgaaggtaa tggaaaaatt catatctata caggtaatta cagtgattat
    25381 cttatttata gagagattca aggaattgaa tttgaagaat ctaaaaggga atcagttaaa
    25441 aaagatgact ctactacccc aaaaaaagaa aaacctaaaa atgataaaaa gcttaagttc
    25501 tcatataatg aacaaaggga gtttgaaaat attgactctg atatagaaaa actcgaatct
    25561 aaaattgcaa cacttgatga aagtactgca aaattttcaa ctgattttac taaactccaa
    25621 gaaattttag atgagaaatc taaactagaa aaagaacttg aatacaaata tgaaagatgg
    25681 gaatatttaa ataatctagc tgatgaaata gctaataata agtaatatta aaataatctt
    25741 aagtttaata agttttgctt taaatctgta atttataaat attttaaagg aagtgtctca
    25801 aaatgaactt tttagtttta tgagacactc ttttttatga gaaatcaaat atattttcat
    25861 catttcaaca atacaaaaga ggcttatctc tattttgaga cagcctcttt cataattacc
    25921 ttctaattta tatgctctaa tatatctata ataattctta ttatatctat atatccagtc
    25981 tttcaatata gtgacaatta acttcaatat gatttaaaaa tactaacaac ctttcataaa
    26041 gtggatgaga ttcttctcca tatttagctt ctaggttctt gcctatttct ctaacagtcc
    26101 tttgaccatc aatttgtaaa aaaacaaaac tggaatattc atctagttct atttttttgt
    26161 atttaggtat tctagctttt aactttctaa aaaacttttg aattttatgg tcctgttttt
    26221 caagtatagt aacaacacga ttttcagcta cttcatattc aagtccatca gtaattttaa
    26281 atacaatatc taaaacatca ttactcttgc tcattatttt ttactttaga taaaacaata
    26341 ggtaatgctg taagcacaac taaaactact aataaagcaa ttgccattga atttgttgca
    26401 gcaaatccac ttggtacctt tggagttaca actccagtta cttgtaatat tatacctatt
    26461 aaaccaatga tagaaccacc agcaacaaga ccagaagata aacttatacc attagaaact
    26521 ttaacttctt tttctttttc agttttagat actaattcaa caaataaacg aattaatgct
    26581 ccaactaata ttatagaagt agttgcaatt ggtaaataga atcctatagc tatagtcatt
    26641 attggtaaat ttaatgcata tagaactatt gccataaata ctccaacaat aatcataacc
    26701 caaggtaaac tacctgacat aataccagaa gttaatgttg acattaagtt tgcttgtggt
    26761 aatgcaaatt gtacattatc accagttgct ctaagttggt cagaaagtaa taaaattact
    26821 cctacaacaa ctataacacc aactatactt gcaattgtaa agtaacgttg catttcgttt
    26881 ttgctaccac caataatata agttactttt tgagattgag tataacctcc agctatagca
    26941 atagcaacaa ctataaatga accaaataaa agtaatgatt tattagcttc taaatcagtc
    27001 catcccatta taacaaacac taatgttaca ataactagag aagctatagt cataccagaa
    27061 actggaagat ttgatgttcc gattgttcct gttaaacgac cagctactat gacaaataat
    27121 aaagataata aaagagatat gatagctcca ataattgcca taacaatgtt accagaaatt
    27181 aagaatgctg ctaaaaatcc aataactact ccacctaata aaagtatcat ttgtatagat
    27241 gaaccttctt ctccttctcc agcacttgat ttagctttaa gtgtttcttt tatagaagcg
    27301 attatagttg gtataagttt tattgcacca ataatacctc cacaaagcat cataccagca
    27361 cctatatatt ttacataact actagatatt gctccaacat ccatttggtt aagaggcatt
    27421 gcaggattat tccataccat agctccatct ttagccatat ctgtaaagta tcctattaaa
    27481 ggtgctatac caaagtttga taaaatactt cctgcaaaca ttgttaaaga aacttcaagc
    27541 cctactataa atccaattcc taaaagtaga ggatttactt caatatcaaa tttccatttg
    27601 tagaatttgc ttcctacaag actcattaca ttgtttgcta catttaagaa tgaaccagtt
    27661 aatacagtaa taaatccact tattacaaat ccaattccca tgtacttaat tgaatctcca
    27721 ccagcctctg aagcaacaag tgtctcagat atagccattg attctgggta cataagttta
    27781 ccatgttctt caactattag atagttgtga actatagcag caattcctat accaaataaa
    27841 actccaccaa ctccaacagc taagccttct aagaatgtaa tttggcttcc aattaatata
    27901 actgctggta atacgaaaat aaatccactt gcaacagatt ctccaccact tgacatacct
    27961 tggattaagt ttttaccaag aatacccttt gaacgagcaa atgctccaac aaaagcagaa
    28021 cctattatag ctcctggaat acctgcggca actgtaagac cggctttcat tccagagtaa
    28081 gttgtagatg cggcaaaaat ggcagctaaa ataataccga taatcagtac agctacattt
    28141 ccaccggttc tggacttatc tgtgatatat ggaacgtaat cttttccact aacctcacca
    28201 taagcaccct taggtaattt tttgttcata aaattttcac cccttttcaa tttgtatata
    28261 ttataacata tatacatata aaacttaact ttttattatt ttacaaatag cgagtcaaaa
    28321 ctactaaaat ttaaccctat attattaatt tctactttta gatattttta tgctaatttt
    28381 ctttttattt ttcaattcta ctaaaaacta ttaagatata aaataaaaaa attttttaat
    28441 gtaaaattaa ttttgtaaaa aaaatgcttt aaaacacgga aaagtataaa aaatacaata
    28501 atcattctta taaataaatt ttcaccatag taaatatata cagaaaattc ttgtttttat
    28561 gcaaattgat tattttataa aaataagtgt cataaagaaa tgtctgaaat cactttatga
    28621 cacctatcat aataaaaaaa ttattctggt aatgctcttt tatttacttt tccgattggt
    28681 gtaactggta tcttatctat cctcattatt attgttggaa tcatatatgg aggtagatgc
    28741 tcatttaaat attctctaat agttctttca tttatcttac cactatcttc ctcatcaatt
    28801 acataatatg cacatatata aatatctcca tcatcatttg ttctcgatat acaaacaaca
    28861 cttttaattt ctgtaatatt atttattata ttttcaatct catatagctc tatcctaaac
    28921 tcatcaattt ttacttgatt gtccatcctt ccaatataat ctaaatttcc atcatctaac
    28981 catcttacta aatcaccagt tctatacatt aacttattat gttcacaagt tttgtaagga
    29041 ttctctacaa atacttgctc tgttagccct tttttattta aatatcctct tgatatttga
    29101 atgcctgata gacatagctc tcctggtgct ccaattggac atagtttgtt ctttttatct
    29161 aatacatatg cataagtatt aaatataggt tttcctattg gtatttttcc atattctctg
    29221 tccacatgaa aagaagtcga tacaacagta aattcggttg gtccatactc attatatagt
    29281 tcataattac gctttctata tccttttaat ctttctcctc cagtctgtac aactctaagt
    29341 gaattattat tgcaattttg tataaataac cttgcaaatg ctgttggcaa tgctagtata
    29401 gttatgtcat tttcctcaca gtattcattt actgtatatt catcaaattt agcttcttca
    29461 ggcataatat ctattgatgc acctacaagc aaataaggga atagttgttt cattacagca
    29521 tcaaaactga aacttaagta tataccacat atatcatttt ctgtagcgct tatagatttt
    29581 acgctataat aacacatatt tactaaactc ttatgttcta tagctatggt ctttggccta
    29641 ccagttgacc ctgaagtaga tatcgtatat gcacaatttg atgatgttat tttttcagtt
    29701 aattcatatc caaacttaaa catttcttca tcatttatat cgagtactat ggtattctca
    29761 tttacaacct tacttttgag ttcatctgta gttatcacta ctttcgcatt actatcttca
    29821 agaaggtaat tcaatctttc ttctggatta cttgtgtcta ttggaaaaaa tgctccccct
    29881 gatttcataa tccctattgc tgtaataatt atgttaatat ctcttggtaa cattacagct
    29941 actatgtctt cactttccac tcctatatga tttaaataac ctgcaagttt atcagttact
    30001 tcatccaatt ctttatatgt taactttcta ttttcatccc tagcagctat attactaggt
    30061 gttaccttaa cttgctccct aaataaatca acataacttt tatcataggg tacttcatat
    30121 tgattattat taaagtcttc caataatact ttttcttctt ccttacatac aaattctaaa
    30181 tcttctatta aaatatcatt atcttctatt atttgctcta ttatataatc aaattgattt
    30241 aaaattcttc taatagtttc tcttttatat atagatggtt tatactttat ttttatttta
    30301 agtgccctct tagtatatac tattacagat aaatcatatc cttttttttc tatgtcataa
    30361 tcatatgata aatcaaaact tttagctata aatattgtgt ttataagctt agtatttaga
    30421 tgatttttat tctctatatc tgataacaaa tagtatccat actctaaaga cctgctccac
    30481 tgccttttta gcttccttac aacatctata aatttttcac tttgtctaac tcttactctt
    30541 attggaatta tattcattgt tttaccaaaa actacatcat cttcatatat atacttttga
    30601 agtataaggc catatatagt ttctatgata gagtctatgc taatattgtt cactttagaa
    30661 tattgaatta acctttcatg tttctcaata ttcatcttaa aaacctcatc tactacttca
    30721 tcagaacaat ctttataaaa aaaatcattt ggaatttttg ctatattcgt ataatcatct
    30781 aacaaatcca tccaataatt catagctcgt tctaaattaa cttcatacat atcaaaattc
    30841 ccccttacta ctaagtctat agaaaaaatt cttttggaat tttattttca caatatataa
    30901 atatattaat aatacatatg caagctctaa aaacatgagc ttgcataaaa ttacacttat
    30961 tatgactact aaattcgtaa tatttacaat gtttaaaata tattataaca catatatgat
    31021 tttatttttc tacatttata taatatata
//
LOCUS       DAMJVK010000041        31029 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.041, whole genome shotgun sequence.
ACCESSION   DAMJVK010000041 DAMJVK010000000
VERSION     DAMJVK010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31029
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.041"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(145..1821)
                     /gene="bclA2"
                     /locus_tag="PC414_001966"
     CDS             complement(145..1821)
                     /gene="bclA2"
                     /locus_tag="PC414_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA2"
                     /protein_id="HDF2464361.1"
                     /translation="MSDISGPSLYQDVGPTGPTGATGPTGPTGPRGATGATGANGITG
                     PTGNTGATGANGITGPTGNMGATGANGTTGSTGPTGNTGATGANGITGPTGNTGATGA
                     NGITGPTGNKGATGANGITGSTGPTGNTGATGANGITGPTGNTGATGATGPTGLTGAT
                     GATGANGITGPTGNTGATGANGVTGATGPTGNTGATGPTGSIGATGATGTTGATGPIG
                     ATGATGADGEVGPTGAVGATGPDGLVGPTGPTGPTGATGANGLVGPTGPTGATGANGL
                     VGPTGATGATGVAGAIGPTGAVGATGPTGADGAVGPTGATGATGANGATGPTGAVGAT
                     GANGVAGPIGPTGPTGANGVAGATGATGATGANGATGPTGAVGATGANGVAGAIGPTG
                     PTGANGATGATGATGATGANGATGPTGATGATGVLAANNAQFTVSSSSLVNNTLVTFN
                     SSFINGTNITFPTSSTINLAVGGIYNVSFGIRATLSLAGFMSITTNFNGVAQNNFIAK
                     AVNTLTSSDVSVSLSFLVDARAAAVTLSFTFGSGTTGTSAAGYVSVYRIQ"
     gene            complement(2199..2738)
                     /gene="hpt"
                     /locus_tag="PC414_001967"
     CDS             complement(2199..2738)
                     /gene="hpt"
                     /locus_tag="PC414_001967"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDF2464362.1"
                     /translation="MDIETKKWEVLYSEEKIKDKLRELGAIIEKDYKDKNLMVVSLLK
                     GSFIFCADLVRNINLPLRVNFMTTSSYGNNEESTGRVKVVSDVTTDIAGYDVLVVDDI
                     TDSALTMDFVLKHLKAKNPASLKCCVLLDKPSRRKVDLVPDYCGFEIEDKFVVGYGFD
                     FGDYYRNVPYIFNVTDEDR"
     gene            complement(3066..4373)
                     /locus_tag="PC414_001968"
     CDS             complement(3066..4373)
                     /locus_tag="PC414_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HDF2464363.1"
                     /translation="MRDIRKELVEMLKAEVKPAVGCTEPVALALACAKAKELLGEEIV
                     ENRMLVSPSIYKNGMCVGIPGTERLGLKIAAALGIVGGHSENGLSVLETLTKEEVKIA
                     EDYMDNTPLSITPADTREKVFIEVVLKGKNHIAKVRIRTKHDNFTFLEKDGEVLLDNE
                     PKVSASNDAAEKAESLMDTVTIQELIKNVEEIDFKDIEFLLDGVKMNEEMAEYGLKQK
                     TGIGVGYGIKKSIEEGLLGNDVINYAMMLTAGASDARMAGVKMPVMSSNGSGNHGLTA
                     ILPIVAYNKKFPQSDERLAKALAISHLVTGYIKNYTGRLSAVCGCGVAASTGATAGIS
                     WLMNGTEKQIEGAIENMIADLSGMICDGAKAGCALKLSSAASAAIQSAIIAKQDCFVP
                     PLNGIVGSSVEQSIQNLGRVSDKGMSITDEIILNVMNDMNKVD"
     gene            complement(4798..6573)
                     /locus_tag="PC414_001969"
     CDS             complement(4798..6573)
                     /locus_tag="PC414_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2464364.1"
                     /translation="MMIMIGLEYIKSDIQNIAEAIVSVLNIDVTIVNKELFRIAGTGV
                     YIDKIGEKVDKYTAFKKSLTEQITILIDDPKSSDICRECYKSGACVEFAEVCCPIICD
                     GDSHGVIGLIAFTKEQAQIIENNKSGLINFLGKMADLISNKLKAQIKTYELELEKKKL
                     ETLLNNMDKAVVSIDVDGKIDKYNSKFKELFNIKDSITGKNIFVELDFIKKPSINNFK
                     KDKSCSFFYKRYGYDSKGIYNINKIVLKNELKGYVIDFIDKRDAIKNYNKMNKDYRIK
                     LENIIGESVAIRNSKKEALIASKSTSTVLITGESGTGKELFARAIHNHSNRVDNPFIA
                     VNCAAIPDNLLESELFGYEEGAFTGAKKGGKLGMFEVAHKGSIFLDEIGDMSLHLQSK
                     LLRVLQEKELNKIGSKSNVSIDVRIIAATNKDLERMVQNGTFREDLYYRLNVIPIKLP
                     SLRERKEDIPLIIKHMVKEYSKKLDKNVIDIDSRVVDAMMEYKWPGNIRELQNIIEYS
                     VNMSSSSIITMNVIPQKIKNTSVEELEVKSDRIIPLDELEKIEIIKALNKYKDYKKDK
                     ELTAKALGISRATLYRKIEKYNIIS"
     gene            complement(6570..6707)
                     /locus_tag="PC414_001970"
     CDS             complement(6570..6707)
                     /locus_tag="PC414_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464365.1"
                     /translation="MEINYELIIIVMLFVILMSIQFTLNKIYLILKEIRQILMLKKNK
                     E"
     gene            complement(6785..7354)
                     /locus_tag="PC414_001971"
     CDS             complement(6785..7354)
                     /locus_tag="PC414_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDF2464366.1"
                     /translation="MKSILCQINKQPVKAKYTSDELECFLDTIEYNVGDKILLVGHIG
                     NLGKRLRGLGTSVTILENSNYEDVCYSLVYNENCNVVKGKLEFLPFDDNQFNKIIIVD
                     QFNVITDCEKASSEIQRVLKGNGEVILEDLNLKNLKVKLNYLKHKFCGSVAKIHYPHE
                     VFNIFSRLNFDGMIKESNKLKYIYVGKRR"
     gene            complement(7538..8173)
                     /locus_tag="PC414_001972"
     CDS             complement(7538..8173)
                     /locus_tag="PC414_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDF2464367.1"
                     /translation="MIAVKDENNVVNLRGELDNKLEFSHEIFGEKFYNMKIKINRLSD
                     SFDILPMTVSERLLQDIDLDKQNLVNVVGQLRSYNKTLNNKNRLVLTVFVREIKSIDE
                     ENKDPNSIFLDGYVCKEPVYRKTPLGREITDLLVAINRPYNKSDYIPSIVWGRNAKFA
                     KNLKVGDRIQLWGRVQSREYEKKIDENNVVKKMAYEVSISKIKKLDENNNE"
     gene            complement(8841..9722)
                     /locus_tag="PC414_001973"
     CDS             complement(8841..9722)
                     /locus_tag="PC414_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HDF2464368.1"
                     /translation="MVAILKALYVVFAAIFAVVFGKDVAKANKEGKLEEQSTPKVAVT
                     GFITDFFDTLGIGSFAPTIAMSKALKLNIPDKKMPGTLNVAHTIPVVTEAFIFTSIIP
                     VDGVTLVSMVVAAAVGSYIGAGIIAKMDERKIQLVMGVTLALTAILMLLGHPWINVLP
                     GGGNELGLTGAKLVIGVIGNFILGALMTAGIGLYAPGMAMVYFLGMSAKVAFPIVMGS
                     CALLMPVASMKFIKEDAYTKKASVIIAICGLIGVFVAAYIVKTLPMDILKALVIVVIA
                     YTSATMIMAANKNKKTA"
     gene            complement(9842..10849)
                     /locus_tag="PC414_001974"
     CDS             complement(9842..10849)
                     /locus_tag="PC414_001974"
                     /EC_number="5.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase"
                     /protein_id="HDF2464369.1"
                     /translation="MKFSRSIQAIDSHTAGEATRIVVGGIPNIKGNSMPEKKEYLEEN
                     LDYLRTAIMLEPRGHNDMFGSVMTQPCCPDADFGIIFMDGGGYLNMCGHGTIGAMTAA
                     IETGVVPAVEPVTHVVMEAPAGIIRGDVTVVDGKAKEVSFLNVPAFLYKEGVEVDLPG
                     VGTVKFDISFGGSFFAIIHASQLGLKIEPQNAGKLTELAMKLRDIINEKIEIQHPTLA
                     HIKTVDLVEIYDEPTHPEATYKNVVIFGQGQVDRSPCGTGTSAKLATLHAKGELKVGE
                     KFVYESILGTLFKGEIVEETKVADFNAVVPKITGSAYITGFNHFVIDEEDPLKHGFIL
                     K"
     gene            complement(10869..11351)
                     /locus_tag="PC414_001975"
     CDS             complement(10869..11351)
                     /locus_tag="PC414_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HDF2464370.1"
                     /translation="MSFVGISTSMKNTSLHIFRDPLLDLIDNDSDIDLLGVVVVGTSE
                     HNEWKTFLATRLGRWIESLRPDGVIITLDMAGNQHIDFTNAIAEFVKSDIPTVGLTIM
                     GADGLVITNPYLDKATIIDYKKTTGYIETEVVGDNHMDEVDVKKALAFLKLKMRKDAK
                     "
     gene            complement(11369..11836)
                     /locus_tag="PC414_001976"
     CDS             complement(11369..11836)
                     /locus_tag="PC414_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HDF2464371.1"
                     /translation="MGIGPSTKETSLHHFRDPLLDIVESDKDVDLLGVIVVGTPDGNE
                     NKTFVGQRTAAWLEAMRVDGAIVSSDGWGNSHVDYANTFEEIGKRDIPVVGVTFNGTQ
                     AKFVVSNQYMDTIVDMNKSKEGIETEVVGENNTNEIDAKKALAFLKLKMRKHG"
     gene            complement(11851..12609)
                     /gene="prdD"
                     /locus_tag="PC414_001977"
     CDS             complement(11851..12609)
                     /gene="prdD"
                     /locus_tag="PC414_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase cluster protein PrdD"
                     /protein_id="HDF2464372.1"
                     /translation="MEEKIMRRLVIKPFHMNEVNFGSKTSIKKDVLTIDLSSIDEIKE
                     REELITDIKVDIIKPGDYDREINTIMDIIPISTKVLGRLGEGITHTLTGVYVMLTGAD
                     EDGNQMHEFGSSEGILKDQMVFGRYGTPSVEDYIIHVDVTLKGGLPFERTLPLAAFRA
                     CDDFIQEIRASLKMEDGRNATQVREYFDKIRPNAKKVVIVKQIAGQGAMYDNQLFSKE
                     PSGFEGGTSIIDMGNVPMIISPNEYRDGALRAMT"
     gene            complement(12689..13414)
                     /gene="prdB"
                     /locus_tag="PC414_001978"
     CDS             complement(12689..13414)
                     /gene="prdB"
                     /locus_tag="PC414_001978"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(12962..12964),aa:Sec)
                     /transl_table=11
                     /product="D-proline reductase (dithiol) protein PrdB"
                     /protein_id="HDF2464373.1"
                     /translation="MSLTTVQGLQSEIFVPITPPPVWTPVTKELKDMTIALATAAGVH
                     LKSDKRFNLAGDTTFRAIPNTATVDEMMVSHGGYDNGDVNKDINCMFPIDRLHELAAE
                     GFIKGVAPMHYAFMGGGGNQHVFTEETGPAIAAKLKEEGVDGVVMTAGUGTCHRTAVI
                     VQRAIEEAGIPTIIIAALPPVVRQNGTPRAVAPLVPMGANAGEPHNIEMQTHILRDTL
                     EQLVAIPSAGKIVPLPYEYKAHV"
     gene            complement(13452..13736)
                     /locus_tag="PC414_001979"
     CDS             complement(13452..13736)
                     /locus_tag="PC414_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464374.1"
                     /translation="MDRLKYISSETFYEGIIVDIKGGGVTIDLKGRLGQFKIPNRMLI
                     TDYELKIGQEVGFMLSYPEVLSPEPNQEYVENIRREKEKQAEMQSKQEEK"
     gene            complement(13817..15697)
                     /gene="prdA"
                     /locus_tag="PC414_001980"
     CDS             complement(13817..15697)
                     /gene="prdA"
                     /locus_tag="PC414_001980"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-proline reductase (dithiol) proprotein PrdA"
                     /protein_id="HDF2464375.1"
                     /translation="MSITLETAQAHANDPAVCCCRFEAGTIIAPENLEDPAIFADLED
                     SGLLTIPENGLTIGQVLGAKLKETLDALSPMTTDNVEGYKAGEAKEEVVEETVEEAAP
                     VSEAAVVPVSTGVAGETVKIHIGEGKNISLEIPLSVAGQAGVAAPVANVAAPVASAAA
                     EVAPKVEEKKLLRSLTKKHFKIDKVEFADETKIEGTTLYIRNAEEICKEANETQELVV
                     DMKLEIITPDKYETYSEAVLDIQPIATKEEGELGSGITRVIDGAVMVLTGTDEDGVQI
                     GEFGSSEGELNTTIMWGRPGAADKGEIFIKGQVTIKAGTNMERPGPLAAHRAFDYVTQ
                     EIREALKKVDNSLVVDEEVIEQYRREGKKKVVVIKEIMGQGAMHDNLILPVEPVGTLG
                     AQPNVDLGNMPVVLSPLEVLDGGIHALTCIGPASKEMSRHYWREPLVIRAMEDEEIDL
                     VGVVFVGSPQVNAEKFYVSKRLGMLVEAMEVDGAVVTTEGFGNNHIDFASHIEQIGMR
                     GIPVVGVSFSAVQGALVVGNKYMTHMVDNNKSKQGIENEILSNNTLAPEDAVRIMAML
                     KNAIEGVEVKAPERKWNPNVKLNNIEAIEKVTGEKIVLEENEQSLPMSKKRREIYEKD
                     EN"
     gene            complement(16126..17886)
                     /gene="prdR"
                     /locus_tag="PC414_001981"
     CDS             complement(16126..17886)
                     /gene="prdR"
                     /locus_tag="PC414_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator
                     PrdR"
                     /protein_id="HDF2464376.1"
                     /translation="MFSIPEVKKVEEVMDTKFTTIDEDTRIEDAIKEMIKSNTKTLMV
                     IDSSDQLKGIISMTDIHNLYEMHKKYEGQPVKLIMKKDVIYVNEGLTLDECRDIMILK
                     NIGILPVLRDNKIIGVLKQEHIRDYLYMHLEDYGLTLKYIIGQIKEGICAINNEGVVI
                     LWNKFMEERYDIKSEDIVGRPMNEFLENTISEKVLNSKVGMSDLYFTDKKENMYALVH
                     ANPIFYKEEFIGVVCTEVDVTEAKILALELEKVNDTLKYLKNEVKNLSKGSFDKILGK
                     SYKLEKSKAIAKQVARTNSSIFIWGESGTGKEVFARAIHDYSERKGQFIPVNCSAIPN
                     ELFESEFFGYESGAFTGASKKGRIGIFELAKDGTVFLDEIADLPLSMQAKLLRVLQEK
                     EIRRVGGDTTIKINPRIISATNKDLSKMVKAEKFREDLYYRLNVVEIKIPPLRERKED
                     IGLLVHSFLEEICKQNNKPVLTISKDVIDIFQNYRWKGNIRELKNTIENIVVLSQNSK
                     IDVDDVPSYMMDSTNNSTEEEEYPLDLTKATQKIEIKNITKALKMSNGNKAKAAKILN
                     IPRTTLYYKIDQYKIDVSKI"
     gene            complement(18293..20467)
                     /locus_tag="PC414_001982"
     CDS             complement(18293..20467)
                     /locus_tag="PC414_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HDF2464377.1"
                     /translation="MKTKIKKSSIISLVIAFSMVFTAFTPIVSYADEVTSNDTILNGE
                     EQSNGQTPDVEKPSDGQVPDGEKPSDSQVPDGEKPSDGQVPDGEKPSDGQIPDGEKPS
                     DGQIPDGEKPSDGQIPDGEKPSDGQIPDGEKPSDGQMPDGEKTSDSQTPDVEKPSDGQ
                     MPDGEKPLDEQTPEEEKPLEEEIVIEEMSLKQDIDKILDMTLSQINKIVYNFWEDEED
                     VKADEQSEINQVFTSEDSFISLWYDKKAKVKNSCLLKEIDGSDRPYHDLRFDDITKTL
                     TFDYVIKGLVGASSKDDKYVIEGEDGEQTAFCYNNHLRPPSSNGKSPYLPAEDFNGQE
                     NQNEEAVKSILYAGSEFDGFGYKQQFNLGGEENELMTYSATQSAIWIILGQMDEQERL
                     KQYQGSINLCDKLIERAKTEEERAEYQKKKEVAINIKAYLEALLKAGREELKPNDTGK
                     PSLSNGLTKINFEKNEDGTYETEAVALVGYSGVVKLRLPDGVTAYDGDGNIIGTGEVE
                     ISTQQKFKLKSVVKPDAKANISAVSYDYIFPKAIQYYKAVLDLGQKDHSSSLPSSKQN
                     LLSYTIEKKNGQEVNFNIGLPTDDVVNPPVPPTDDTNGHKPKPSPPIDDTVINPPVPP
                     MDDTIINPPVPPTEDAVLNPPVPPTEDAVLNPPVPPTEDTVLNPPVPSVSDTVEKTPE
                     LSRDDTIIKSPKTGDETQLMSYVFISVIAICGLAYQCKIKRN"
     regulatory      complement(21049..21134)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(21539..22852)
                     /gene="prdC"
                     /locus_tag="PC414_001983"
     CDS             complement(21539..22852)
                     /gene="prdC"
                     /locus_tag="PC414_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase-associated electron transfer
                     protein PrdC"
                     /protein_id="HDF2464378.1"
                     /translation="MNKLYSILLKQHVGGPDKPVVSVGDVVKKGTLIAEPTGLGANIY
                     ASVSGKISEINDQAIVIEADEVQEDTFEPLKGEGILDLIKEAGVVGMGGAGFPTHVKL
                     NIDLNGGTILANAAECEPLLAHNIKEIEERPEIVYQGIKYAMEVTNAGKGMLAIKSKH
                     PKAIEAFKKVIKPGDNIEVAELVDMYPMGEERAIVRDVLGKLLEPTQLPSEANAVVIN
                     VETLTRIVEAVEQKRPVISKNITVVGQLNSGKESIVFEDVPIGTTVGELIERAGGIKG
                     EYGEIILGGPFTGKATTLDAPITKTSGGIIVTMPFVNEKRKMGLLVCACGPNEERMRD
                     IATKMGVTDIVSVQKCKQAQEIKGALKCENPGHCPGQAQKCIEFKKAGAEVILIGNCT
                     DCSNTVMGSAPKLKLGVYHITDHVMRTVNHPLIRRIKGDVRLKIK"
     gene            complement(23241..24005)
                     /locus_tag="PC414_001984"
     CDS             complement(23241..24005)
                     /locus_tag="PC414_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2464379.1"
                     /translation="MKREVKRTKALIYSVLFFSLLLVLIFVFVYGIKYICSKSDNSKM
                     PIYKVDTNEKKIALSFDVAWGTDNMDDILKILDKHNVKATFFLVGSWVDDNEEIVKAI
                     DEKGHEIGNHSNTHANLKEISKEDITKEIETTSEKIYNITGKRTNLFRPPFGDVNNKA
                     MDICSDLGYKVIKWDVDSIDWKELGPNHVIEKVIKESQPGSIVLFHANINDVDNYLDT
                     IISRLKKDGYSLVKISDLLYKDNYVVDSNGVQKTKN"
     gene            24296..24499
                     /locus_tag="PC414_001985"
     CDS             24296..24499
                     /locus_tag="PC414_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HDF2464380.1"
                     /translation="MSNSNRTVVPEAKAALNQMKLEIANEIGLSNYENIDKGNLTARE
                     NGYVGGYMTKKLVEMAERQMAGK"
     gene            24690..25226
                     /locus_tag="PC414_001986"
     CDS             24690..25226
                     /locus_tag="PC414_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HDF2464381.1"
                     /translation="MFENSSEELAYHKLLILYILEKIKMDLTNSQITQVVLETEMMNY
                     FSLQQLLSQLMESKFLTTYKDSDREYYALTQRGVESLEYFFNRIPTSVTEKIDKYIDS
                     NKENLLADTQVKSSFVKQSDNEFIVNLRVIENQSNLIDLNLNVSSEKQAKLICNNWKN
                     NASYMYAEVIDLLIRDIH"
     gene            complement(25230..26096)
                     /locus_tag="PC414_001987"
     CDS             complement(25230..26096)
                     /locus_tag="PC414_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HDF2464382.1"
                     /translation="MKIYISNYLSKSISIVDYSTLELEKEIVLEDNIYPHHFCIEKEK
                     NLIYIPSSSNGILYVLDLSNDKIIDTVSIGGSLSQVVLSDNELFVANEDSNSIYVLDK
                     NTLNPIGIIGVDNMPHGFDFDRESKKLYVPCINSIVCIDTINKNIQKKINMDFRAWHI
                     ELDKQKKEMYISTLDGKLIIIDEVSMDIKKVLYEFLLPVEIRFNYSGKKVYVADLGYN
                     NVRILDYTTSRYIGNIEINGIPQGLEISKDEKLLFVSDTQENSVKVYETANNKLIKEI
                     KVGKEPTTIICL"
     gene            26244..26489
                     /locus_tag="PC414_001988"
     CDS             26244..26489
                     /locus_tag="PC414_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HDF2464383.1"
                     /translation="MKVTFNPSGVCCREMIFEVDENNVIVDAEFIGGCNGNLSGLRSL
                     IIGQNALEVADKLNGIDCGGKGTSCPDQLSKAIRQSI"
     gene            complement(26603..27901)
                     /locus_tag="PC414_001989"
     CDS             complement(26603..27901)
                     /locus_tag="PC414_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDF2464384.1"
                     /translation="MKYEEALEYISQTNKFGIRLGLENIGKLLELLGNPQETLNIIHV
                     AGTNGKGSVCSFVSNILRECGYKVGLYTSPYLETFTERIRVNGQNIPQDDVARIIELI
                     KEKIEIMVKEGYAYPTEFEVVTAMAFYYYSEQKVDFVALEVGLGGRYDATNIITKSLV
                     SVIVSISLDHTGILGDTIEKIAYEKAGIIKENGVVLVYDQTDEAKDVIKSVCKEKKAK
                     YIEVDFDDINIKKSDINSQIYDCTVMKETYRDLEIKLIGEHQINNSILAISVIKYLKD
                     INKLANINEESIRKGLINTKWPGRIEKIKENPIFIIDGAHNEDGAKSLAKALDKNFKG
                     RKLTLLIGMLEDKDIDSVLEILLSHFNKVITTTPNNPRAINSDILREKVLKYVDDVTS
                     KHEIEDAVNYTLETSSEDDIIISAGSLYMIGTVRTLVKKL"
     gene            complement(28034..29065)
                     /locus_tag="PC414_001990"
     CDS             complement(28034..29065)
                     /locus_tag="PC414_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HDF2464385.1"
                     /translation="MENICDMNNKVKVAVLDTGIDKEHDYLKDNLVGGIAFECMHDYI
                     FISDKFDDEDGHGTACASIIKKEYEDVELFVIKILRKDGITNIKVLEEALRYLLDTNI
                     RLINLSLSVIGVESVKGLFEVCYELFRKGKIIVCSLANDFDLSYPAMFNNVIGVRGFT
                     LDGDDSFWYNKKYDVQCVMDSNSYISCDINNSYRLPPKCNSYLSAKLTGKIAKILSEK
                     PGITISDLNDKLESLATRNHWDSCDFDECSRIVNFKLDLYDKENALFMKVADVVRDCL
                     DIEENNEKLFKGSLFNKEIGLVYDNCFNLLKKLEHRFDIKFNYMDISKYDLVSIYTLT
                     ELVERYKNL"
     gene            complement(30111..30821)
                     /gene="rgaR"
                     /locus_tag="PC414_001991"
     CDS             complement(30111..30821)
                     /gene="rgaR"
                     /locus_tag="PC414_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgaR"
                     /protein_id="HDF2464386.1"
                     /translation="MFRVVICDDEKIQRSILKEFTEKLLSERCLNYQILEFSCGEDLI
                     SKYPEKIDIIFLDIQMKDINGIEVAKKIRKFDEKVEIIFTTAFSEYAPQGYEVRAYRY
                     LVKPIEYSNFAMGVNLCIDNLQRKKERYIVLNSKKGFNRILIDSILYVETVKRDLVVH
                     TIGRDYKANMSMKQAEKLLCENGFFRCHTSFIVNLRRIEDLRDNMITINEQFIPISKY
                     RLKDLKVALTSLLCDIMY"
BASE COUNT         9346 a         5769 c         3517 g        12397 t
ORIGIN      
        1 atattatcct atttattggt atatgaaatc atattgttta ttattttata gtcttttcat
       61 catataaacc tattaaatat tattctattt attggtatat gaaatcatat tgtttattat
      121 tttataatct tttcatcata taaactattg tattctataa actgatacat atccagctgc
      181 agaagtacct gtcgtgcctg aaccaaatgt aaaacttaaa gtaacagctg ctgctctagc
      241 atcaactaaa aagcttaaac ttacacttac atctgatgaa gtaagtgtat ttactgcttt
      301 tgcaataaag ttattttgag ctactccatt aaagttagta gtaattgaca taaatcctgc
      361 aagtgaaagt gtggcacgta taccgaaaga tacattgtat atccctccaa ctgcaagatt
      421 tatagtacta cttgttggaa aagttatatt agttccattt ataaatgatg aattaaatgt
      481 cactaatgta ttattcacta aacttgaaga ggacactgta aattgtgcat tgtttgctgc
      541 taacactcct gttgctccgg tcgctccggt tggacctgtt gctccatttg ctcctgttgc
      601 tccggtcgcc cctgttgctc ctgtcgctcc atttgctcca gttgggcctg ttggtcctat
      661 cgctcctgct actccatttg ctcccgttgc tcctactgct ccggttgggc ctgttgctcc
      721 atttgcccct gttgctcctg tcgctcctgt tgctcctgct actccatttg ctccggttgg
      781 acctgttgga cctattggac ctgctactcc attcgctccg gttgctccta ctgctcccgt
      841 tgggcctgtt gctccatttg cccctgttgc tcctgtcgct ccggttggac ctactgctcc
      901 atctgctccc gttgggcctg ttgctcctac tgctcctgtt ggacctattg ccccagctac
      961 tcctgttgct cctgtcgctc ctgttggacc taccaaacca tttgctccag ttgctccggt
     1021 tgggcctgtt gggcctacca aaccatttgc tccagttgct ccggttgggc ctgttgggcc
     1081 tgttggacct accaaaccat ctggacctgt tgctcctact gctcctgttg gacctacctc
     1141 tccatctgct cctgttgctc cggttgctcc tattgggcct gttgccccag ttgttcctgt
     1201 tgctcctgtc gctcctatac ttccggttgg acctgttgct cctgtatttc ctgttgggcc
     1261 tgtagcacct gttactccat ttgctccggt tgctcctgta tttcctgttg gacctgttat
     1321 tccatttgct ccggttgctc cggttgctcc agttagtccg gttggacctg ttgctccagt
     1381 tgctcctgta ttccctgttg gacctgttat tccatttgct ccagtcgctc ctgtatttcc
     1441 tgttggtcct gtagagcctg ttattccatt tgctccagtt gctcctttgt ttcctgttgg
     1501 acctgttatt ccatttgctc cggttgctcc tgtattccct gtcggacctg ttattccatt
     1561 cgctccagtc gctcctgtat ttcctgttgg tcctgtagag cctgttgttc catttgctcc
     1621 agtcgctccc atatttcctg ttggtcccgt tattccattc gccccggttg ctcccgtatt
     1681 tcctgttgga cctgttattc catttgctcc ggtcgctcca gttgcacctc taggccccgt
     1741 cggtcctgtt ggaccagtag cacctgttgg ccctgttgga cctacatctt gatataaact
     1801 tggacctgaa atatcactca tataattaat cctccttttt taaagttaga gtattactct
     1861 ataaatattg agtattactc tatataatta tatgaatcga caatttctta tgtgataatg
     1921 tatctatttg cttatatcaa catatatgct ttgattcttg aatttcatta cttttttgta
     1981 aaatctttta caattattag tactctttta caattattaa tacaattaaa aaactcattt
     2041 tccatcaatt atttaaaatt tttttattat gttataaaaa atagataatt aaattgcata
     2101 aaattaattt aatactactt taattacttt gtaaataaaa aaacctcaac acgattaata
     2161 aaaatcatat taaggcatcc acaaaattat ataattgctt atctatcttc atcagtaaca
     2221 ttgaatatat aaggaacatt tctataataa tcaccaaaat caaacccata tccaaccaca
     2281 aacttatctt ctatttcaaa tccacaataa tctggcacta aatcgacttt tcttctgctt
     2341 ggtttatcca aaagaacaca acattttaaa ctagcaggat ttttagcttt taaatgtttt
     2401 aacacaaaat ccatagtaag tgcagaatca gtaatatcgt ctacaaccaa aacatcatat
     2461 cctgctatat cagtagtcac atctgaaact actttaaccc ttccagtact ttcttcattg
     2521 tttccataac tagatgtagt cataaaatta actctaagtg gtaaatttat atttctaact
     2581 aagtctgcac aaaatataaa gctacctttt aatagagaaa ctaccataag atttttatct
     2641 ttataatctt tttctataat tgctcctaat tcccttaatt tatctttaat tttttcttct
     2701 gaatacaata cttcccattt ttttgtttct atatccataa aaagacctcc aattgtaata
     2761 atccttctta attttataag agattgctca ctttttcaat actcttattc aatattagtc
     2821 aatattattg agtttttatg ttcattcaac aattattaac aatgtatctt atattttaag
     2881 tagttcatat atccacacta aattacatac tatatatttt agacaaaaaa agaaaaaact
     2941 tgcactaaca agttttttct attatacata aatttataca taatcatatt ttattagagg
     3001 gtactaatta aatttattta tgctattttt gttatacctc ataatacaaa acatattata
     3061 aagtactagt caactttatt catatcattc attacattta agattatctc atctgttata
     3121 gacatacctt tgtcacttac tctacctaag ttttgtatac tttgctcaac acttgaacca
     3181 actataccat ttagtggtgg tacaaaacaa tcttgcttag ctattatagc actttgtata
     3241 gctgcagatg ctgcagaaga taattttaat gcacaaccag ctttcgcacc gtcacatatc
     3301 attccactta agtcagctat catattttct atagctcctt ctatttgctt ttcagtacca
     3361 ttcataagcc aagagatacc agcagttgct ccagtagaag cagcaacacc acatccacat
     3421 acagcagata atcttcctgt atagttctta atataaccag ttactagatg agatatagct
     3481 aaagcttttg ctagtctttc atcagattgt gggaattttt tattataagc aacgattgga
     3541 agaattgctg ttaatccatg atttccactt ccatttgaac tcataacagg catttttacc
     3601 cctgccattc ttgcatctga agctccagca gttaacatca ttgcataatt tataacatca
     3661 ttacctaaaa gaccttcttc tatagacttt ttaattccat acccgacacc aataccagtt
     3721 ttttgtttta atccatattc agccatctct tcattcattt ttactccatc tagtaaaaat
     3781 tctatatctt taaaatcaat ttcttctaca ttttttatta attcttgtat agttactgta
     3841 tccattaaac tttcagcttt ttctgccgca tcatttgaag ctgatacttt tggttcattg
     3901 tctaataata cttctccatc tttttctaag aacgtaaaat tatcatgttt agttcttatt
     3961 ctcactttag caatatgatt ttttcctttt aacacaactt ctataaatac tttctctctt
     4021 gtatcagcag gtgttattga tagtggagta ttatccatat aatcctcagc tattttaact
     4081 tcctcttttg ttagagtttc taacacactt aatccatttt cagaatgtcc acctactata
     4141 cctagagctg ctgcaatttt taatcctaat ctttcagtac ctggtatacc tacacacata
     4201 ccatttttat agatactagg actaactaac atacgatttt caactatttc ttctcctaat
     4261 aattcctttg cctttgcaca tgctagagct aaggctactg gttcagtaca ccctactgct
     4321 ggtttaactt ctgcctttaa catttccact aattcttttc ttatatctct cattatatat
     4381 aaacctccca attaagttta gatttagtac tcaaataatt agttaaataa tttcttatct
     4441 atatttatag caaaatacat gccatatttt ttatatcttt ttatattttt agtcttatat
     4501 tctataacca ttggtatgac tgcctctatt tatgtatttt ttttatttgt atagagtatt
     4561 tatattcttt aaaatggaga aacatatgac aatttcgcaa aaagacaaaa aaaagtctca
     4621 aaatgagatt ctatatcatt ttgagacggc tcaaatatat tattttttaa cttataaaaa
     4681 ccatttttta attcataaaa aaagattctg caaaacactt atagtataag tttaaagctt
     4741 tctttaaagt tatgaattta gaaaaattta aattttatat agctttttat tatattacta
     4801 tgaaattata ttgtattttt caatttttct atataatgta gctcttgata ttccaagtgc
     4861 tttagctgtt aattctttat cttttttata atctttatac ttatttaaag cttttattat
     4921 ctcaatcttc tctagttcat caagtggaat tatcctatcg cttttaactt ctaactcttc
     4981 tacagaggta ttcttaattt tttgaggaat tacattcatt gtgattattg aagaagaaga
     5041 catattcaca ctatattcaa taatattctg caactctctt atattccctg gccacttata
     5101 ttccatcata gcatcaacaa ccctgctatc tatgtcaata acatttttat ctaatttttt
     5161 tgaatactct ttaaccatat gcttgattat aagtgggata tcttcttttc tctctcttaa
     5221 acttggtaat tttataggta taacatttaa tctataatat aaatcttctc taaaagtgcc
     5281 attttgaacc attctttcta aatctttatt tgtagcagca ataattctta catctattga
     5341 aacatttgat ttacttccaa ttttattaag ttccttttct tgtaatactc tcaataactt
     5401 tgactgtaaa tgcaaactca tatctcctat ttcatctaaa aatatacttc ccttatgagc
     5461 tacttcaaac attccaagct ttccaccttt tttagctcca gtaaaagctc cctcttcata
     5521 tccaaataat tcactctcta ataaattatc cggtatagca gcacagttta cagctataaa
     5581 aggattatct actctattgc tatggttgtg tatggctctt gcaaatagct ccttacccgt
     5641 tccactttcc ccagtaataa gtactgttga tgttgattta gaagctatta gggcttcctt
     5701 tttagaattt cttatagcta cactttctcc aattatgttt tccaatttta ttctataatc
     5761 tttattcatc ttattgtagt tttttatagc atctctttta tctataaaat caataacata
     5821 tccctttaac tcatttttta atactatttt attgatgttg tatattccct tcgaatcata
     5881 tccgtatctt ttataaaaaa aactacagga tttgtccttc ttaaagttat ttatagaagg
     5941 tttctttata aaatctaact cgacaaatat gtttttgcca gttatactat cttttatatt
     6001 gaaaagctct ttaaattttg aattatattt atcaattttt ccatccacat caatagacac
     6061 aacagctttg tccatattgt ttaatagtgt ctctaatttt ttcttttcta attcaagctc
     6121 ataagtttta atttgtgctt ttaatttatt agatattagg tctgccattt tgcctaaaaa
     6181 gtttataaga ccacttttat tattttctat aatttgtgct tgttcttttg taaaggctat
     6241 aaggcctata acaccatgag aatcgccatc acatataata ggacaacaca cctctgcaaa
     6301 ctctacacaa gccccacttt tatagcattc cctacatata tcactactct ttgggtcatc
     6361 aattagtatt gtaatctgtt ctgtaagtga ttttttaaaa gccgtatatt tatctacttt
     6421 ctcacctatt ttgtctatat atactcctgt acctgcaatc ctaaataact ccttatttac
     6481 tatagttaca tctatattga gtactgatac tatagcttca gctatatttt gaatatcaga
     6541 ttttatatat tctaaaccta tcataatcat cattccttat ttttctttag cataagtatt
     6601 tgtcttattt ctttaagaat caagtatatc ttatttaaag taaattggat tgacatcagg
     6661 attacaaaga gcataacaat tattattaac tcataattta tttccatcta aacatctcct
     6721 tataacaaaa gactaggctt aacctagtct tttatattat atccatattt atattttaac
     6781 tcatttatct tctttttccc acataaatat attttaattt atttgactct ttaatcatac
     6841 catcaaagtt taatcttgaa aatatattaa atacttcatg tggatagtga atctttgcaa
     6901 cagaaccaca aaatttatgt ttaagatagt ttaacttaac ttttaaattt tttaaattta
     6961 aatcctctaa tataacctct ccattgccct ttaacactct ttgtatttca gatgaagctt
     7021 tttcacaatc tgtaataaca ttaaattggt caactattat tatcttgttg aactgattat
     7081 catcaaatgg tagaaactct aacttacctt taactacatt gcaattttca ttatacacta
     7141 gagaataaca gacatcttca taattactat tctccagtat agttacagaa gttccaagac
     7201 ctctcaatct cttgcctagg tttcctatat gaccaacaag caatatttta tcacctacat
     7261 tatactcaat tgtatccaaa aaacattcta attcatcaga tgtatattta gctttaactg
     7321 gctgtttatt tatctgacat aatatgcttt tcacaatgct tcacctcttc atttcctcaa
     7381 ttataaatta tgtacagatt acattataat aaatggcgag caactattca agagatattt
     7441 cattagtatt tttttaaaca atctttatac attttaatat gatattatac caaatttatt
     7501 aggattttat tcataaaaat cagtctattt tttatgttta ttcattgtta ttttcatcca
     7561 atttttttat tttcgatatg gatacttcat atgccatttt tttgaccaca ttattttcat
     7621 ctattttttt ctcatattct ctactttgaa ccctacccca aagctgtatt ctatcaccaa
     7681 cctttagatt ctttgcaaat ttagcatttc taccccaaac tattgatggt atataatcag
     7741 atttattata aggtctgttt atagcaacta ataaatcggt tatttccctt cctaaaggtg
     7801 tcttcctata cacaggttcc ttacatacat atccatctaa aaatatacta ttagggtctt
     7861 tattttcctc gtctatactt tttatttctc tgacaaacac tgtaagcact aatctatttt
     7921 tattatttaa tgttttgtta tatgatctta attggcctac gacattgact aaattttgtt
     7981 tatcaaggtc aatatcttgg agaagtcttt ctgatacagt cattggaagt atatcaaaag
     8041 aatcgcttag tctgtttatt tttattttca tattataaaa tttttctcca aatatctcat
     8101 ggctaaattc tagcttattg tctaactccc ctcttaagtt tactacgtta ttctcatctt
     8161 taacagctat catattttat ccctccacaa ttgttaattc ttatatttct ggtatcagct
     8221 tatataataa atatatttat aagggatgta ttttattaca aatctttgat tgctgtttaa
     8281 aagtttttat ttagttataa atttcattta atatttcact tttataatta taaaattatt
     8341 atctttttgt accacatata ctaattaaac tacctatttc agttttgtat taattataaa
     8401 aatcctatta tataattaat aagtcatata taacttaaat ttattcactc tatataagca
     8461 acttcaacga attaaattta ttgaaataat tcagatgaat attgagtcat tataaactta
     8521 tttttcttaa ttataagtta tattagactt attacaggtt atatttttat taatctgtat
     8581 taatatatga acatttattg aacttattga ttataaaatt taaatattta gctttaaatt
     8641 atgctttatt ctttaaaaat acaacgtttt ttagattaaa tctactaaat cactatatat
     8701 attacataat tggcatgtta attgctttat attaatttag taaataaaaa actgccctaa
     8761 aatattaatt caatatttta aagcagttta taatctttat tattagctgt aatatttaat
     8821 aaacactaag tagtctttaa ctaagcagtc tttttgtttt tatttgcagc cattatcatt
     8881 gtagcagaag tataagctat aactactatt actaatgctt ttaatatatc cattggtaga
     8941 gtttttacta tatatgcagc tacgaaaact cctattaaac cacatatagc tattataact
     9001 gatgcttttt tagtatatgc atcttctttt atgaatttca tacttgcaac tggcattaat
     9061 aatgcacaag aacccataac tattgggaat gcaacttttg cagacattcc taagaaataa
     9121 accattgcca tacctggagc atatagacct ataccagctg tcattaaagc tcctagtata
     9181 aagttaccta ttacacctat tactaattta gcaccagtaa gacctaattc atttcctcca
     9241 cctggtaata cgttaatcca aggatgacct aataacatta atattgctgt taatgctaaa
     9301 gttacaccca taacaagttg tatttttctc tcgtccatct tagctattat tcctgcacct
     9361 atatatgaac caactgctgc tgccacaacc atagatacta aagtaacacc atctactggg
     9421 ataattgatg taaatatgaa tgcttctgta acaactggaa tagtatgtgc tacgtttaat
     9481 gtaccaggca tttttttatc tggtatattt aactttaaag cctttgacat tgctattgtt
     9541 ggagcaaatg accctattcc taatgtatca aagaagtctg ttataaatcc tgttacagca
     9601 acttttggag tactttgttc ttctagtttc ccctctttgt ttgctttagc tacgtcttta
     9661 ccaaacacaa ctgcaaagat tgctgcgaat actacatata atgcctttaa gattgctacc
     9721 ataatccatc ctcctatttt tttatttttt catatattct gaatcttata gcctaaaagc
     9781 tatagaatag caaccgacaa gtggctacta ttctatttta atttcctaat ttatttatta
     9841 cttatttaag aataaatcca tgtttaagtg ggtcttcttc atctattaca aaatgattaa
     9901 atccagttat ataagcagaa ccagttattt taggtactac agcattgaaa tctgcaactt
     9961 tagtttcttc aactatttca cctttgaata aagttcctaa tatactttca tatacgaatt
    10021 tttctcctac ttttaattca cctttagcat gtaaagtagc taatttagca cttgttccag
    10081 ttccacatgg agatctgtca acttgacctt gaccaaatat aactacattt ttgtaagtag
    10141 cttctggatg agttggctca tcatatattt caactaagtc tacagttttt atatgagcta
    10201 aagttggatg ttgtatttct attttctcat ttattatatc tctaagtttc atagctagtt
    10261 cagttaattt accagcattt tgaggttcga tttttaaacc taattgactt gcatgtatta
    10321 tagcaaagaa gcttccacca aatgatatgt caaatttaac agttccaaca cctggtaagt
    10381 caacttcaac accttcttta tataagaaag ctggtacgtt taagaatgat acttctttag
    10441 cttttccatc tacaactgta acgtctcctc ttattattcc tgctggagct tccataacta
    10501 catgagttac aggctctact gctggaacta cacctgtttc tatagctgct gtcatagctc
    10561 ctattgtacc atggccacac atgttaaggt atccgccacc atccatgaag attattccaa
    10621 agtcagcatc tgggcaacaa ggttgagtca ttacagaacc aaacatatca ttatgtcctc
    10681 ttggctctaa cattatagca gttcttaaat aatctagatt ttcctctaaa tattcttttt
    10741 tctcaggcat agaatttcct tttatgttag gtattccacc tacaactatt ctagtcgctt
    10801 cacctgctgt atgagagtct atagcttgta tacttctgct aaatttcata tcaaattccc
    10861 ccttaggttt attttgcgtc tttacgcatt tttaatttta ggaaagccaa tgctttttta
    10921 acatcaactt catccatgtg attgtctcca actacttctg tttctatgta gcctgttgtc
    10981 tttttataat caattatggt agctttatcc aaatatggat tggtaattac taatccatct
    11041 gctcccataa tagtaagacc tactgtaggt atatctgatt ttacaaactc tgctatagca
    11101 tttgtaaaat caatatgctg gtttcctgcc atatctaaag ttataatcac tccatcaggt
    11161 cgcaatgatt caatccatct tccaagtcta gttgctaaaa atgttttcca ttcattgtgc
    11221 tctgatgtcc caactacaac cactcctaac aagtctatgt cagaatcatt atctatcaaa
    11281 tctagtaacg ggtctctaaa tatatgaagt gatgtgtttt tcattgatgt agaaattccc
    11341 acaaaactca ttcccttact taaatttttt atccatgttt tctcatttta agttttaaaa
    11401 atgccaaagc ttttttagca tctatttcat ttgtgttgtt ttctccaact acttctgtct
    11461 caataccttc ttttgatttg ttcatatcaa ctatggtatc catatattga ttgcttacaa
    11521 caaacttagc ttgagtccca ttaaatgtaa ctccaactac aggtatatct ctttttccta
    11581 tttcctcaaa tgtgttagcg tagtcaacat gtgagtttcc ccaaccatct gatgaaacta
    11641 tagctccatc tactctcatg gcttccagcc aagcggccgt tctttgtccc acaaaggttt
    11701 tattttcatt tccatctgga gttcctacta ctattactcc aagtaaatct acatccttat
    11761 cactttcaac tatgtctaaa agtgggtctc taaagtgatg aagagatgtt tcttttgttg
    11821 aaggtcctat tcccataata tccacctctc ttaagtcata gctctaaggg caccatctct
    11881 gtattcatta ggagatataa tcataggtac atttcccata tctataattg atgttcctcc
    11941 ttcaaatcca ctaggttcct ttgagaataa ctgattgtca tacatagcac cttgacctgc
    12001 tatttgtttt actatgacaa ccttttttgc atttggtcga attttgtcaa aatattcacg
    12061 aacttgagta gcatttcttc cgtcttccat tttcaaagac gctcttattt cttgtataaa
    12121 atcatcacat gctctgaatg ctgcaagtgg aagtgttctt tcaaaaggta aacctccctt
    12181 taaagttaca tctacatgga ttatataatc ttcaactgat ggagttccat atctcccaaa
    12241 taccatttgg tctttcaaaa ttccttctga agaaccaaat tcatgcattt gatttccatc
    12301 ttcatctgca ccagttaaca ttacatatac acccgttaaa gtatgtgtaa taccctctcc
    12361 cagtctgcct aaaacttttg ttgatattgg tataatgtcc ataatagtat taatttctct
    12421 gtcataatct ccaggtttta taatgtctac ttttatgtct gttattaact cttctctttc
    12481 ttttatttca tctatacttg ataaatctat tgtaagtaca tctttcttta tagaagtttt
    12541 tgaaccgaaa ttaacttcat tcatatgaaa cggttttata acaagtcttc tcattatttt
    12601 ttcttccata atatccacct ttgttcttaa aacccctctc ctaaccgtgt aggagaaggg
    12661 ttctatattt tcaaatataa attacatttt aaacgtgagc tttatattcg tatggtaatg
    12721 gaactatctt accagcagat ggtattgcaa ctaattgctc taatgtatct cttaatatat
    12781 gtgtttgcat ttctatatta tgtggttcac cagcattagc acccattgga actaatggag
    12841 caactgctct aggagttccg ttttgtctaa ctactggagg aagagctgct attattattg
    12901 taggtattcc agcttcctct attgctctct gcacgatcac ggcagttcta tggcaagtac
    12961 ctcagccagc tgtcataact acaccgtcta ctccctcttc cttaagttta gcagcgatag
    13021 caggaccagt ttcttcagtg aagacatgtt ggtttcctcc accacccatg aatgcatagt
    13081 gcataggagc tactcctttg ataaatcctt ctgcagctaa ttcatgtaat ctatctatag
    13141 ggaacataca gtttatatct ttgtttacat ctccattgtc atatccacca tgtgatacca
    13201 tcatttcatc aactgtagct gtattaggta tagctctaaa tgtagtatct cctgctagat
    13261 taaatctctt gtcagatttt aaatgaacac cagctgctgt agctaaagct atagtcatat
    13321 cttttaattc tttagttaca ggagtccaaa ctggaggagg tgttattgga acgaatattt
    13381 cagattgaag tccttgtact gttgtaaggc tcatatattt cctctccctt ctattttcaa
    13441 acgattattt attatttttc ttcttgtttt gattgcattt ctgcttgttt ttctttttct
    13501 ctcctaatat tttctacata ctcttgatta ggctctggac ttaatacctc aggatagctt
    13561 agcatgaatc caacttcttg acctatctta agttcgtagt cagttataag cattctatta
    13621 ggtattttaa attgtccaag tctacctttt aggtctatag tcactccacc gcctttgatg
    13681 tcaactataa taccttcata aaaagtctct gatgagatat attttaacct atccattata
    13741 attctacctc tctatactgt tttaaacttg gatctaaatt tatgttattg tctaaatctt
    13801 agttagtatc taaattttag ttttcgtctt tttcgtatat ttctcttctc ttcttactca
    13861 ttggtagaga ttgctcattc tcttctaata ctattttttc tcctgtaact ttttctatag
    13921 cttctatgtt atttaattta acatttggat tccattttct ttcaggagct ttaacttcta
    13981 caccttctat agcattttta agcatagcca ttattctaac agcatcttct ggagctaaag
    14041 tgttgttaga taatatttca ttctctatac cttgcttaga cttattgttg tctaccatgt
    14101 gagtcatgta tttattacca acaactagag caccttgaac agctgagaaa cttacaccaa
    14161 ctactggtat acctctcata cctatttgct ctatgtgaga tgcgaaatct atatggttgt
    14221 ttccgaaacc ttcagtagtt actacagctc catcaacttc catagcttca actaacatac
    14281 ctaatctctt agatacatag aatttctcag catttacttg tggagaacca acaaatacaa
    14341 cacctactaa atctatttct tcgtcttcca tagctcttat tactaatggc tctctccagt
    14401 aatgtcttga catttctttt gatgcaggtc ctatacaagt taatgcatgg ataccaccat
    14461 ctaatacttc aagtggagat aatacaactg gcatgtttcc taagtcaacg tttggttgag
    14521 ctcctaatgt accaactggc tcaactggta atattaggtt atcatgcatt gcaccttgtc
    14581 ccattatttc ttttataaca acaactttct ttttaccttc tcttctgtat tgctctatta
    14641 cttcttcatc aactactaaa gagttgtcaa ctttctttaa tgcttctctt atttcttgag
    14701 ttacatagtc aaatgcacgg tgagcagcta aaggtcctgg tctttccatg ttagttcctg
    14761 cttttattgt tacttgacct ttgatgaata tttcaccttt gtcagcagca cctggtctac
    14821 cccacattat agtagtattt aactcacctt ctgaagaacc aaattcacct atttgaactc
    14881 catcttcatc tgtaccagtt aataccatta cagctccatc tataactcta gttatacctg
    14941 aacctaattc gccttcttct ttagtagcga ttggttgtat atctaataca gcttcactgt
    15001 aagtttcata tttatcagga gttattattt ctaacttcat atctacaact aactcttgag
    15061 tttcattagc ttctttacat atttcttctg cgtttctgat gtataaagta gttccttcta
    15121 ttttagtttc atcagcaaat tcaactttat ctattttaaa gtgtttttta gttaagcttc
    15181 ttaaaagttt cttttcttca acttttggag ctacttctgc tgctgcactt gccactggag
    15241 cagcaacgtt tgctactgga gcagcaactc ctgcttgacc agctactgat aaaggtatct
    15301 ctaagcttat gttcttacct tcacctatgt gtattttaac tgtttcacct gcaactcctg
    15361 tgcttactgg aactactgct gcttctgata ctggagctgc ttcttctact gtttcttcta
    15421 ctacttcttc tttagcctct cctgctttgt atccttctac gttatctgta gtcattggag
    15481 aaagtgcatc taaagtttct tttaacttag ctcctagtac ttgacctata gttaatccat
    15541 tttctggtat tgttaataat ccagaatcct ctaagtctgc aaatattgct ggatcttcta
    15601 agttttctgg cgctataatt gttcccgctt caaatctaca acaacaaact gctgggtcat
    15661 ttgcatgggc ttgagctgtt tctaaagtta ttgacatagt ataaaatacc tccttacatt
    15721 attttcttat ttattatttt aaatctcaat tcctcaacag gtaacccctt aactgagaaa
    15781 ttaagtaaat taaaacctac ataatttgtt atgattaata ttattttttt ctcacttaaa
    15841 gtcactttat attataagca attcctatgc caacttatta acaaaaagat tttttctata
    15901 atagattctt catctatcgc tatttttagc caaatattat ataaatattt atttaaaata
    15961 tgttattttt tgtatttgta aacattttct caaacaaaaa aagtgtcaat taatcaacac
    16021 tttttcattt tttttaacac atctatatac acaacactta tataagcgtt ggctttacta
    16081 ggttttaaat taaagtttat cattttgtca aaatttagtc atgtgtcata ttttagacac
    16141 atctattttg tattggtcta tcttataata caaagttgtt ctgggtatat ttaatatctt
    16201 agctgcttta gctttatttc cattagacat tttaagtgcc ttggttatat tctttatttc
    16261 aattttttga gttgccttcg ttaaatctaa aggatactct tcttcttcag tactattatt
    16321 tgtagaatcc atcatataac ttggtacatc atccacatct attttagaat tttgtgacaa
    16381 tacaacaata ttttcaatcg tattttttaa ttctctaata ttacctttcc atctataatt
    16441 ctgaaatata tctattacat ctttgctaat tgtaagaaca ggcttattat tttgtttaca
    16501 tatctcctct aaaaagctat gaaccaataa tcctatgtct tcttttctct ctctaagtgg
    16561 aggaatcttt atttcaacaa catttaatct atagtataaa tcttctctaa atttttctgc
    16621 ctttaccatt ttactcaaat ctttattggt agctgatatt attctaggat ttatttttat
    16681 tgtggtatct ccaccaactc ttcttatttc tttttcctgt aatactctaa gcaactttgc
    16741 ttgcatacta agaggtaaat cggcaatttc atctaaaaat acagttccat cttttgcaag
    16801 ttcaaatata cctattcgac cctttttact tgctcctgta aacgcacctg attcataccc
    16861 aaaaaactca ctttcaaata actcatttgg tatggcacta cagttaacag gtataaattg
    16921 accttttctc tcactataat catgaatagc tcttgcaaat acttctttac ctgtaccact
    16981 ttcgccccaa ataaatatgc tactatttgt tctagccact tgttttgcaa tcgctttcga
    17041 tttttccaat ttataacttt tacctaaaat cttatcaaaa ctacctttag ataaattttt
    17101 gacttcgttc tttagatatt ttaatgtatc atttactttt tcaagttcaa gagctaatat
    17161 cttagcttct gtaacatcaa cttctgtaca tacaacaccg ataaattctt ctttataaaa
    17221 tataggattt gcatgaacta atgcatacat attttctttt ttatctgtaa aatacaagtc
    17281 actcattcca actttactat ttagtacttt ttctgaaata gtattttcca aaaattcatt
    17341 catcggtcga ccaactatat cttcagactt tatatcatac ctttcctcca taaacttatt
    17401 ccacaagata acaacacctt cgttatttat agcacaaatt ccttctttaa tttgacctat
    17461 aatatacttc aaagttaatc cataatcttc taaatgcata tatagatagt ccctaatgtg
    17521 ctcttgtttt agaactccaa taattttatt atctctaaga acaggaagaa tccctatatt
    17581 ttttaatatc attatatctc tacattcatc taaggttaaa ccttcgttga catatattac
    17641 atccttcttc attataagtt ttactggttg accttcatac tttttatgca tctcataaag
    17701 attatgtata tccgtcatgg atataatgcc ttttaattgg tcagatgaat ctattaccat
    17761 tagagttttt gtgttgcttt ttatcatttc ttttattgca tcttcaattc ttgtgtcttc
    17821 gtctatagtt gtaaattttg tatccattac ctcttctact ttttttactt ctggtatcga
    17881 aaacataaag tccccccttt atagtagtac ctcatcaaaa cttatttata tatatttatt
    17941 ctagcatatt ataataatta tttattaaat aattattatt aaaatttatt aagtaattag
    18001 catttaaatt tatataatca ctttaaaatt tataattaaa tttatacaaa tgaaatcaaa
    18061 tgaattatac ccttagtata ttattttttc aatactattt tcaccttcaa aacatttttc
    18121 tattcatcaa acaattaatg aatgcctgta tccaatcata aatttaagaa tataattaaa
    18181 atatatattt ttaaacaaac tatatttgca tattatattt acataaaaaa gccccaaaat
    18241 gacttataaa tcaatttgag actttttatt ttaaacttaa taataatcta gattaatttc
    18301 tttttatttt acattgatac gctaatccac aaattgcaat gacactaata aatacatatg
    18361 acattagctg agtttcatca cctgtttttg gtgattttat tattgtatca tctcttgata
    18421 gttctggagt tttttccact gtatcactta ctgatggtac tggtggattt aacactgtat
    18481 cctctgttgg tggcactggt ggatttagca ctgcatcctc cgttggtggc actggtggat
    18541 ttaacactgc atcctccgtt ggtggtactg gtggatttat tattgtatca tccattggtg
    18601 gtactggtgg atttattact gtatcatcta ttggtggtga tggttttggc ttatgtccat
    18661 ttgtatcatc tgttggtggc actggtggat ttacaacatc atctgttggt agacctatat
    18721 taaagttaac ttcttgacca ttttttttct ctattgtata acttaataaa ttttgcttac
    18781 tagatggtaa tgaagatgaa tggtcttttt gtcctaaatc aagcactgcc ttataatatt
    18841 gaatagcttt tggaaaaata taatcataag atacagcaga aatattagct ttggcatctg
    18901 gttttacaac actcttaagt ttaaatttct gttgtgttga aatttctact tcacctgtac
    18961 ctattatatt accatctcca tcataagcag ttactccatc tggtagtctt aactttacta
    19021 caccactata acctactaag gctacagctt ctgtttcata agtaccatct tcattttttt
    19081 caaagttaat cttagttaaa ccattactca aactaggttt tcctgtatca tttggcttta
    19141 actcttcacg acctgctttt aacaatgctt ctaaataagc ttttatattt atagctactt
    19201 ctttcttctt ctgatactca gctctttcct cttcagtttt tgctctttct ataagtttat
    19261 cacaaaggtt tatagaacct tgatactgct taagtctttc ttgttcatcc atttgtccaa
    19321 gaataatcca aattgcactt tgagtagctg agtaagtcat caattcattt tcttctccac
    19381 ctaaattaaa ttgttgctta tatccaaatc catcaaattc agaacctgca tatagtatag
    19441 atttaactgc ttcttcattt tggttttctt gaccattaaa atcttctgct ggtaaatatg
    19501 gactctttcc attactactt ggtggtctta aatgattatt gtaacaaaat gcagtttgct
    19561 ctccatcttc accttctatt acatatttgt cgtcttttga gcttgctcct acaagaccct
    19621 ttattacata gtcaaaagtt aaagtctttg taatatcatc aaatcttaaa tcatgataag
    19681 gtctatctga accatctatt tcttttagta aacaagaatt ttttactttt gcttttttgt
    19741 cataccataa gctaataaat gagtcttctg aagtaaaaac ttggttaatt tctgattgtt
    19801 catctgcctt tacatcttct tcatcttccc aaaagttata tactatttta tttatttgag
    19861 ataaagtcat atcaagaatt ttatcaatat cttgttttaa actcatttcc tctataacta
    19921 tttcttcttc caatggtttt tcttcttctg gagtttgctc atctaatggt ttctccccat
    19981 ctggcatctg accatctgat ggtttctcta catctggcgt ctgactatct gatgtcttct
    20041 ctccatctgg catctgacca tctgatggtt tctccccatc tggtatctga ccatctgatg
    20101 gtttctctcc atctggtatc tgaccatctg atggtttctc tccatctggt atctgaccat
    20161 ctgatggttt ctccccatct ggtatctgac catctgatgg tttctcccca tctggtacct
    20221 gaccgtctga tggtttctcc ccatctggta cttgactgtc tgatggtttc tccccatctg
    20281 gtacctgacc gtctgatggt ttctctacat ctggtgtctg accatttgat tgttcttctc
    20341 cattaagtat tgtatcattg ctagtcactt cgtcagcata agatactatt ggtgtaaatg
    20401 cagtaaacac catactaaaa gcaattacta aactgattat acttgatttt ttaatttttg
    20461 ttttcaaaat tctcctgata ctaaaaacat atattgtatt cacttaatat aattaaaatt
    20521 attaaatatt atatgaatct tattcaataa tacatcttta gtgtgttcaa ttagggtcgt
    20581 tactcctaac cagttctcct atgaacttct taatatttct attaagcaca gactatatca
    20641 ttacctaacc ataaattagg tactccccat ttccccacac ttgtaggtac gagatttctc
    20701 tctagtcgtt gaagtttctt cttttcgaag cttacctgct gattatccat tttcaaggca
    20761 cttaggattt aaccttatgc tatctaacta attttttcta ctttcgtaac ctcaagtgct
    20821 tattaaatgc acactctatc acacttatcc ttatttcatg attatgttgt ggtttagtta
    20881 gctttaggaa cttccagcaa ttaaaggagt tttgggcaga tatctgccac tctacactcg
    20941 ttatgaatgc agggagcatt tcaaaaatat atttatatct atcttctatt aattacttat
    21001 ttatgtattt attttatata tttttgacaa ttaaaagtta ctcctattta tatttatagc
    21061 aggttgcact actaactcca tatatctttt aagtgcccag gtttaagata tacttcacag
    21121 tttctataaa taggtttttt aatttttctt ttttaacttt ttaagtgcac ttttttaaca
    21181 actcaataaa atataaatta gtatcctaga tacttatcta ataaaagaca aaaattagtt
    21241 tttataataa aaaagatact atagagtatc ttcaaatatc acaaaaacct tttactctat
    21301 cggatagttg acttaatata atatgcatat taatacaaac cacatacttg agtatcatta
    21361 tttttgtcca atattaattg taatagctat tattatcatt atacaatatc tagcatattt
    21421 tgtctatttt ttccatttat tttacaatat atatattgcc ctcaaaatac attaaaaaac
    21481 atccttagaa gtaactttta agttttactt ctaaagatgt ctaacttcta tttatctatt
    21541 attttatttt taatcttacg tcacctttta ttcttcttat taatgggtga ttaactgttc
    21601 tcattacatg gtcagtaata tgatatacac ctaattttaa ttttggtgca gaacccatta
    21661 ctgtgttact acagtcagta cagtttccta ttaatataac ttctgctcca gctttcttaa
    21721 attcaataca tttttgagct tgtcctggac agtgtcctgg attttcacat tttaatgcac
    21781 ctttaatttc ttgagcttgt ttacattttt gaactgatac tatatcagta actcccattt
    21841 tagttgctat atctctcatt ctttcttcat taggtccaca tgcacaaact aaaagaccca
    21901 tttttctctt ttcattaaca aatggcatag taactattat accaccacta gttttagtta
    21961 ttggagcatc taaagtagta gctttaccag tgaaaggtcc tcctaggatt atttcaccat
    22021 attcaccttt tattccacca gctctttcaa ttaattctcc gacagttgtt ccaattggca
    22081 catcttcaaa cactatagat tcttttccac tgtttaattg accaactaca gttatatttt
    22141 tagatataac tggtcttttt tgctcaacag cctctactat tctagttagt gtttctacgt
    22201 ttattactac tgcattagcc tctgaaggta attgagttgg ctcaagtaat tttcctagaa
    22261 cgtctcttac tattgctctc tcttctccca ttgggtacat atcaactagt tctgcaactt
    22321 ctatgttgtc tcctggcttt attacttttt tgaaagcttc tattgctttt ggatgcttac
    22381 tttttattgc tagcatacct tttccagcat tagtaacttc catagcatat tttatacctt
    22441 gataaactat ttctggtctt tcttctattt cttttatatt atgagctaat aaaggttcac
    22501 attcagctgc atttgctaat attgttccac catttaaatc tatgtttaat tttacatgag
    22561 tagggaatcc tgctccaccc attcctacta ctccagcttc tttaattaag tcaagtatac
    22621 cttctccttt taatggttca aaagtatctt cttgtacttc atcagcttca attactatcg
    22681 cttggtcatt tatttcactt atttttccac taacactggc ataaatattt gcccctaatc
    22741 cagttggttc tgcgattaga gttccttttt ttactacatc tccaacactt actactggct
    22801 tatctggacc accaacgtgt tgttttaata aaatagaata tagtttgttc aactagtacg
    22861 cccccttaag tatattaatt tttttataag ctgttcaaat aatgttaatg ctatttttct
    22921 attataaggt cttaacatat tgtttatttc tatagaacat atcgcttatt aacaaagcaa
    22981 tttctatgcc aacttttaaa caaacttata ccctttggaa atactgtctt acagcattca
    23041 ttaattcttc taaattttta aacccatcaa aaaaaagtca attattagac acttttttaa
    23101 tcaatttaat ctataattat tgatattgca tacatgtata aaaagtgtca aaaaatagac
    23161 accgtctttt tatcgacact tttctaacat atactatata atttatcaca actttactta
    23221 aaatatacat ttattactta ttaattctta gttttttgca ctccattgga atcaactaca
    23281 taattatctt tatacaacaa atcagatatt ttgactagac tgtatccatc tttttttagt
    23341 cgacttatta tagtatccaa atagttatct acatcattta tattagcatg gaataaaact
    23401 attgaaccag gttgagattc ttttattact ttttcaatta catgatttgg tccaagctcc
    23461 ttccagtcta tagaatctac atcccactta attactttat aacctaaatc actacaaatg
    23521 tccatagctt tattgtttac gtccccaaaa ggaggtctaa ataaattagt cctttttcct
    23581 gttatattat atattttttc tgatgtagtt tctatctctt ttgttatgtc ttcttttgat
    23641 atttccttca aattggcatg agtatttgaa tgatttccaa tttcatgtcc tttttcatct
    23701 atagctttta caatttcttc attatcatcc acccaactac caactaaaaa gaaggtagct
    23761 tttacattat gtttatctaa aatttttaaa atatcatcca tattatcagt tccccatgct
    23821 acatcaaaac tcaaagctat ttttttctca tttgtatcaa ctttatatat aggcattttt
    23881 gaattgtcag atttagaaca tatgtatttt attccataaa caaaaacaaa tattaacact
    23941 aataataatg aaaaaaataa aacactatat atcaaagctt tagtcctttt aacttctctt
    24001 ttcatactat tccccctttg aaataatttc ttctatattt aaaatttatt ttttttcagc
    24061 tcaaaaaagt actgtaatat ttaatttata agacaataac taaaaaaata aaacctatat
    24121 tgacctaatt aaactctcta aaatactcat ttatttaatt tttttatatt ttttacattt
    24181 ttacctttat ttttctgtat atattatttt tattttggag aaattagtat taacatcaca
    24241 taaataaaag tgttgttaga catcataaac taattatttg aggaggaaat ttactatgtc
    24301 aaattcaaac agaacagtag ttccagaagc aaaagctgct ttaaaccaaa tgaaattaga
    24361 aatagctaac gaaataggtt tatctaacta tgaaaacata gacaaaggta acttaacagc
    24421 tagagaaaat ggatatgttg gtggatatat gactaagaaa ttagttgaaa tggctgaaag
    24481 acaaatggct ggaaaataaa tagtaaattt tgacacctgg gtatcaacag ggctgctaat
    24541 tagcagccct gtttttctat atttatttat aaaattgttt ttatatgaat tattaaagat
    24601 taataataca atacaaattg ttcttttaat tttgtacaaa ttagtttaat atattatata
    24661 tagaaatagt ttgacggagg atgatattta tgtttgaaaa ctcgtctgaa gaattagcct
    24721 atcacaaact tttaatttta tatatattag aaaaaattaa aatggattta actaattctc
    24781 aaataactca agtagtatta gagactgaga tgatgaatta tttttcatta caacagcttt
    24841 tatctcagct tatggaatct aagtttttga ctacatataa agactctgac agagaatatt
    24901 atgctcttac tcaaagaggc gtagaaagtc tagaatactt ctttaatcga atacctacta
    24961 gtgttactga aaaaatagac aagtatatag attctaacaa agaaaatcta ctagcagata
    25021 ctcaagtaaa gtctagtttt gtaaaacaaa gtgataatga atttattgtc aaccttagag
    25081 tcatagaaaa tcagtctaac ttaatagatt taaatctaaa tgtttcatca gagaaacaag
    25141 caaaacttat ttgcaataac tggaaaaata atgcatctta tatgtatgcc gaagttatcg
    25201 atttacttat aagagacata cattaaagcc tacaaacaaa ttatagttgt aggctctttt
    25261 cctaccttga tttcttttat taatttgtta tttgcagttt cataaacttt tactgaattt
    25321 tcttgtgtat ctgatacaaa tagtaacttt tcatctttag atatttctaa tccttgggga
    25381 atcccattta tttcaatatt tccaatgtat ctactcgttg tataatctaa aattcttaca
    25441 ttattataac ccaaatcagc aacataaact tttttgccac tataattaaa tcttatttct
    25501 actggtaata aaaattcata taataccttt tttatatcca tgcttacttc atctattatt
    25561 ataagctttc catcaagtgt tgatatatac atttctttct tttgtttatc aagttctata
    25621 tgccaagctc taaagtccat atttattttc ttttgtatat ttttattaat tgtatctata
    25681 catactatcg agtttataca cggtacatat aactttttgc tttctctatc aaagtcaaat
    25741 ccatgtggca tattatcaac tccaataatt cctattggat ttaatgtgtt tttatctagt
    25801 acatatatac tattagaatc ttcatttgcc acaaacaatt cattatcgct aagtacaact
    25861 tgactaagac ttcctcctat agagacagta tcaattattt tatcattact taaatctaag
    25921 acatagagta tcccattact tgaactaggt atgtatatta aatttttttc tttttctatg
    25981 caaaaatggt gtggatatat attatcttct aatactattt ctttttctag ttcaagtgta
    26041 gaataatcaa ctatgctaat actctttgat aagtagttag aaatatagat tttcatataa
    26101 cccctcctct ctacaataca tatactacaa taatatatta tttcattagc ttgtattatg
    26161 ctttatagtt gcattattac gcatatattt ggtattatta ttaatgttaa aaaaagaaat
    26221 aatatttaat ggaggtcttt ttaatgaaag taacatttaa tccaagtggt gtatgttgta
    26281 gagaaatgat ttttgaagtt gatgaaaata atgtgattgt tgatgctgag tttataggag
    26341 gatgtaatgg taatttatca ggacttagaa gcttaataat tggccaaaat gctcttgaag
    26401 ttgctgataa attaaatggt attgactgtg gtggaaaagg tacttcttgc ccagaccaat
    26461 tatctaaagc tatacgtcaa tctatataat tacttatata aacatcagta aaattattaa
    26521 aaacttattt tatataaaaa gagttgtcca ttttttagac aactcttttt tatattttta
    26581 tttgtatact tattttataa aattataatt tctttactaa agttctcact gtaccaatca
    26641 tatataatga acctgcactt attataatat catcctcact tgatgtttct aatgtataat
    26701 ttactgcatc ctctatctca tgctttgatg ttacatcatc tacatatttt aatacttttt
    26761 ctcttaatat atctgaattt atggcccttg ggttatttgg tgttgttgtt attaccttat
    26821 taaaatgtga caacaatatc tctaaaacac tatcaatatc tttatcttct aacataccta
    26881 ttaataaggt taattttcta cctttaaaat tcttatcaag tgcttttgcc aaagattttg
    26941 ctccatcttc attatgtgca ccatctatta taaaaattgg attttccttt atcttttcta
    27001 ttcttcctgg ccatttagta tttataagtc cttttcttat agattcttca tttatatttg
    27061 ctaatttatt tatgtctttt agatatttta ttacactaat tgccaagata gaattgttta
    27121 tttgatgttc gcctatcaat tttatttcaa ggtctctata agtttctttc attacagtac
    27181 aatcgtatat ttgagaattt atatctgatt tttttatgtt tatatcatca aaatcaactt
    27241 ctatatattt agcttttttc tctttacaaa ctgactttat aacatccttt gcttcatcag
    27301 tctggtcata tactagtact actccatttt cctttattat tccagccttt tcgtacgcta
    27361 ttttttctat tgtatctcca agtatacctg tatggtccaa acttatagat actatcacac
    27421 ttacaagtga ttttgttatt atatttgtag catcatatct gccacctaaa ccaacttcta
    27481 atgccacaaa gtctaccttt tgttctgaat agtaataaaa tgccattgca gtaaccactt
    27541 caaattctgt cggatatgca tagccttctt ttaccataat ctctattttt tcttttatta
    27601 actcaattat tctagcaaca tcatcctgtg gtatgttttg tccatttact cttatacgct
    27661 cagtaaaagt ttctaaatat ggagatgtat ataaaccaac tttatatcca cactctctta
    27721 gtatattaga aacaaaagaa cacacagaac cttttccatt agttccagct acatgtatta
    27781 tattcaaagt ttcttgtgga tttcctaata attctaataa ctttccgata ttttctaatc
    27841 ctaatcttat accgaattta ttagtttgtg atatgtactc taatgcttct tcatatttca
    27901 ttatctaact ccccctgtat tggcattttc tcttatgcta tctaattata gtatattttt
    27961 tacttttaat ctaccgacaa agaatacata taatttaata ccttctacat taaatttagc
    28021 tacaaatctt tatttataag tttttatatc tttccactaa ttcagtcagt gtatatattg
    28081 aaactaagtc atattttgag atgtccatat aattaaactt tatgtcaaat ctatgctcta
    28141 gctttttcaa cagattaaaa caattatcat aaaccaagcc tatctcctta ttaaaaaggc
    28201 tacccttaaa gagtttttca ttattctctt caatatctaa gcaatcccta acaacgtcag
    28261 caactttcat aaaaagagca ttttctttgt catacaaatc caatttaaag tttacaattc
    28321 tactacactc atcaaaatca caactatccc aatgatttct agttgctaat gattcaagct
    28381 tatcatttaa atcagatatt gttatgcctg gtttttctga taatatctta gctattttac
    28441 cagtaagctt tgctgataaa taactattac attttggtgg taatctatag gaattattta
    28501 tatcacaact tatatatgaa ttgctatcca taacacattg aacatcatat tttttgttgt
    28561 accaaaatga atcatctcca tctaaagtaa atcctcttac acctataaca ttgttaaaca
    28621 tagctggata acttaagtca aaatcattag cgagtgaaca tactattatt tttccttttc
    28681 taaacaattc atagcaaact tcaaataagc ccttaacact ttctactcct atgactgata
    28741 aacttaaatt aattaatctt atgttagtgt ctaaaaggta tcttaatgct tcttcaagaa
    28801 cttttatatt tgtaatacca tcttttctca aaatttttat tacaaataac tctacatctt
    28861 catactcttt ctttattatt gatgcacaag cagttccatg accatcttca tcatcaaatt
    28921 tatctgatat aaagatataa tcatgcatac attcaaaagc aattcctcca actaaattat
    28981 cttttaagta atcatgttct ttgtctattc ctgtatctaa tactgcaacc ttaactttat
    29041 tattcatatc acatatattc tccattacaa aagtttttaa tatatctatg ttaatagttc
    29101 atcttaaact ttgatttaag ttgcacaatt tatcttttaa atgctatatt tcttgctaaa
    29161 aacataaaat aagtacacca tatatttaat ttatatttat tatatcaatt attatcatta
    29221 aatggttaaa tgttaagata aaatttttag atttatatca caatttagaa ttatttgtaa
    29281 aaaaggagat tatcttttta ttagacaatc cccttgtata ttttattaat atttatctat
    29341 gattaagcat caattttatt ttatactttt tgattttaat actatacatc taaatcttat
    29401 ttagggggaa caattattta tatgatataa aaatgctaat ttgaatgaac ttgtcttata
    29461 atcagtttta gaaaatacac cattctacaa agtatgaatc aaaaggtgaa aaaatactaa
    29521 aaataataaa tcaacatcaa ttttaatcaa tatatgaaaa acttatacca agtatgtata
    29581 aaaactaata taaatattat tttaacaact aatacttaca cctttatata ttatatcata
    29641 tttcaataaa taaattgcat aaaaggaaat cttttcatag aattaaacta tttattaatt
    29701 cattactata gtttcaataa actataagat gtcgctttaa tatttatatt gtactccatt
    29761 aaatattaat ataattaatt aaagtatgcg tagctaaatg aaacaaatat ctatagagtt
    29821 ttataaataa tcattatttt aaaaaggtaa tcatattcta aaaatacata taatgcatat
    29881 aaaaatcaat aatagtatat tgataatcaa gctactaatt aataatcatg ctactataat
    29941 ggaacctcta catatacatg ttaaaccata ttaaaaatct ataaactgct attttgcata
    30001 ataaaactgt cttaatataa ataacatttt atatattaag acagtttcaa tcaatagccc
    30061 tgtgatgaca ttaaatcttt tttacgatat tttcaattac ctttttaaaa ttaatacatt
    30121 atatcacata acaagcttgt taatgctact tttaaatctt taagtctata cttacttata
    30181 ggtataaatt gttcattaat agtaatcata ttatccctaa gatcttctat tcttcttaaa
    30241 ttaacaataa aacttgtatg acatctaaaa aagccatttt cacacagtaa tttttctgct
    30301 tgtttcatgc tcatattagc tttataatct ctacctattg tatgtacgac taaatctctc
    30361 tttacagttt caacatataa aatagaatct atcagtattc tattaaatcc ttttttacta
    30421 tttaaaacta tataacgctc cttctttctt tgaaggttat caatacaaag atttacaccc
    30481 attgcaaagt tactatactc tataggcttt actaaatatc tataagcacg aacctcataa
    30541 ccttgggggg catactctga aaaagccgtt gtaaatataa tttctacttt ttcatcaaac
    30601 tttcttattt ttttagccac ctcaattcca ttgatatctt tcatttggat atctaaaaat
    30661 ataatatcaa ttttttctgg gtatttggat attaaatctt ctccacagga aaattccagt
    30721 atttgataat ttagacatct ttcagacaaa agtttttctg taaactcttt tagtatgctt
    30781 ctttgtattt tttcgtcatc acatataact accctgaaca taatatcacc tccaaatcta
    30841 aaatatcatt tagtttactt tatttcaatt tccaaactgt actcactagc tttcaaattt
    30901 aaatttcttt ataaatttaa attgtcgatt aaactactta tagaaattct ctttaatatt
    30961 taaacttaaa ttacgataaa ttcctatact tttctagata tattatgtaa aaaaatatag
    31021 taaaataaa
//
LOCUS       DAMJVK010000042        30537 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.042, whole genome shotgun sequence.
ACCESSION   DAMJVK010000042 DAMJVK010000000
VERSION     DAMJVK010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30537
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.042"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     regulatory      55..157
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            251..1627
                     /locus_tag="PC414_001992"
     CDS             251..1627
                     /locus_tag="PC414_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDF2464387.1"
                     /translation="MQTTNSSTMQKLFPILTNKNVDMKKEIIAGVTTFLTMAYIIAVN
                     PNILSETGMPAGALVTGTCLAAAFGCILMGVVANLPFALASGMGLNAFFAYTVVLQMG
                     VPWEVALTAVFVEGIIFIVLSVSGVREAVVNAIPKNMKLAVTGGIGIFIALIGLVNSG
                     IVIGDQATLIKMGRFTPAVIITCVGLIIIAVLDKKRVKGSILFGIVVSSLLAWAFAFI
                     NPEYAQKLGIYLPGGLFKFESLAPIAGKIDLGYVLHPTNIGGFLVVVCTFLFVDFFDT
                     VGTLVGVCSKANMLDEKGNVPNVGRALLTDAVATTVGAGLGVSTVTTYVESSTGVIAG
                     GRTGWTAITVGILFLAAMFFSPVFIAIPSCATAPALIYVGYLMLGTVKDIEFDNITEG
                     VPAFVTIACMALTYSIGDGLTLGILTYVFVNIFYNIFGAKKAEDKKHVSVVMIVLAIL
                     FIIKLWLL"
     gene            complement(1741..2886)
                     /locus_tag="PC414_001993"
     CDS             complement(1741..2886)
                     /locus_tag="PC414_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF2464388.1"
                     /translation="MNTFEQLKISSTLIDGLKKQDITSPTEVQSLVIGNIIQNKDLLI
                     NSQTGTGKTLAYLLPIFEKIDTSKRETQALILAPTHELVMQITNQVELLAKNAELSVT
                     SLALIGEVNIQKQIKNIKAVKPHIVIGSCGRVLDLIKQKKLKSHNIKTIILDEVDNLL
                     NGKNITCIEDIIRTTLRDRQIIGCSASLTDSTIKICDKLMKEFEIIKTKEKSQINPNI
                     NHSYLLGEIRDKFTFLRKALAATNPKKAIVFVNNEKNIEVLVSKLNYHNYKAIGIFGN
                     MEKEDRKNAINKFKLGKAKILITTDLSARGLDIVDVSHVFNLDFPKSKNEYLHRCGRT
                     ARGNRSGNTISIITKKELDIIKDLQKEFNIVITPKTLQNGELIDIIK"
     gene            3139..3846
                     /locus_tag="PC414_001994"
     CDS             3139..3846
                     /locus_tag="PC414_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2464389.1"
                     /translation="MLKVINVSKSYKNKKVVSSVSFDVNDGELFGFIGHNGAGKTTTI
                     KAIVGIHDFEEGEVLINSKSIKKQPVECKKEMAYIPDNPDLYESLTGIQYLNFIADIF
                     EVAKDKREELIKYYSNKFELNKALGDLISSYSHGMKQKLAIISALIHSPKILILDEPF
                     VGLDPKASFILKEIMREFCDKGGCIFFSTHVLEVAEKICDKIAIIKDGNIIAYGTTEE
                     VKGSNSLESIFMELIEK"
     gene            3893..5488
                     /locus_tag="PC414_001995"
     CDS             3893..5488
                     /locus_tag="PC414_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2464390.1"
                     /translation="MSNLSILIKTNIINEFKLNALKKADGREKQKIMGMMLSVVIIAG
                     LLIFYVTSLSLSLVDILKQINQLEMLLIFGFGLSTLTTIVTSLYKTSSYLFQAKDLDL
                     LTSLPIKESTVLASKILMLVGANYLFSSICMIIPAVVYFLNSDVSITYFPYLILLFLA
                     LPLLPIVASSIIFLFIGQISSRIKYKNLVLIIGSIILMLLYTLLMSRLNSMSVEMLKN
                     SVSISETIRKVYPPIYYFVDALKTGSIVSFLAFIAISIIPFFMFVTLFAKKFKDINAK
                     MSEGYKSKNYEFKGQKTSRPSKALLKKEFKRYTSSFIYFLNSSAGLLFLMVGTILILL
                     FGADKVGELLKLNIDFNLIKIQLLGVFFFIIVMNCTTYCSISLEGKNLWILKSSPIDE
                     REIFWSKILMNFLLNAPLSILCLILVSIRLDFEIQFIIVSICLIVSFSMFVALMGLLS
                     NLLYPNLDWKNEVAVVKRSASMIVTMLVSLAYVIILGGIYVLLGISFLNIYIILASIL
                     TLILDFMIWRIISTKGVRIFKNL"
     gene            5591..6673
                     /locus_tag="PC414_001996"
     CDS             5591..6673
                     /locus_tag="PC414_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HDF2464391.1"
                     /translation="MRKLSKREKDFIQYGFLLFLICITTYLVLTTLDIKLIPKIIRLV
                     NKTYIFLGILTIIVYMIIESIVTHLIINAVQKTKVKFIGIKMATMGFYYNLVTPFASG
                     SQPMQIYALTKYDVSLSKSVAIVTNKTVVFQSTVTVFCGILILLNKSLLMEQVHSVKV
                     LITFGMIMNVSMLLGGLLIVFSPKQVKMIAEVLINFLSKFKTFKFLDSKRDRINHYID
                     DYNYSIKIFIKNVRILVLSILLTVLQLIAYFSIVYCVYKASNLRGVSYVHIMTLQVFL
                     YMAISPIPTPGNVGANEVTFFTMFSNIIPKELLGYSVLLYSGFVYYFILIVSGLFTFG
                     THYTLNSWKKENKVNSNLDKVIEKLN"
     gene            6822..6983
                     /locus_tag="PC414_001997"
     CDS             6822..6983
                     /locus_tag="PC414_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III"
                     /protein_id="HDF2464392.1"
                     /translation="MKQDKQNEKQSEISKIKQSNDNKLEIAQELHVSKNIGNMLGYRI
                     DKKKMNRPS"
     gene            7324..8592
                     /locus_tag="PC414_001998"
     CDS             7324..8592
                     /locus_tag="PC414_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDF2464393.1"
                     /translation="MEASIHTIASNNLLIIFAIVSITGIICSKLSEIIKVPDVVLYLL
                     VGILIGPSFLKFIDIRGFQIENQLILTFGSAFILYLGGKEISLKVLRNVKISVFLLAT
                     LGVVISAFIMQQVIGFTFGISAMTALLAGTIIASTDPATLVPIFNQVKIKDRVKQTVI
                     SESAFNDATGAILTSAVLAVILSNKFSLGENIYELGTMVIVGVLVGLITGILLLKLVN
                     DKPYGIFKDFAPIISVISVVVAYELSTKLGGSGYMSCFIVGIITGNKKNFKIWLSQKS
                     YDADFYVAETLGTICRMAIFIILGSQVNLAQLSKYFMPSVITVLVLMFIARPLCVLVC
                     TLVDREAKWSKSEILFMMWVRETGVIPAALCGIISAMKVPGYEVISSVVFMTILITLV
                     IQGSTTKLVAKKLGLLEEEVINISEKVSTF"
     gene            8787..9575
                     /locus_tag="PC414_001999"
     CDS             8787..9575
                     /locus_tag="PC414_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HDF2464394.1"
                     /translation="MFTVMDIVQPDTVEEAYSILNKRKTNQVIGGSAFLRMGKKRIGT
                     GIELSKLNLDYIKEYEDYVEIGSMTTFRTLETSSIIKNNFGRIIEDSVKNIIGVQFRN
                     VVTVGATVFSKYGFSDLIVALLSLDTEVELYNIGRISLEEFLNRDYEKDLLIKIYIKK
                     TNKNASYKSIRNAKSDYPILNVSVSKHMEQFKLCVGARPQKATIAKQASEFLSNNEIN
                     EINIDKAVEIASEELTFGSNMRASREYRKAMSKVLLKRAIMEVI"
     gene            9575..10024
                     /locus_tag="PC414_002000"
     CDS             9575..10024
                     /locus_tag="PC414_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HDF2464395.1"
                     /translation="MLVKININGKKKSVDIEPEEYLVDTLRKVGNLSVKRGCDTGCCG
                     LCTVLIDKKPTLSCATLTLRALDKEITTIEGLENEVKEFSEVLVKEGAEQCGFCSPGF
                     ILTVLAMKDELENPTDDEIKHYLTGNLCRCTGYMGQLRAIKTYLEVK"
     gene            10028..12292
                     /locus_tag="PC414_002001"
     CDS             10028..12292
                     /locus_tag="PC414_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF2464396.1"
                     /translation="MNIVGKGIKKIDGMSITTGKPVYTDDLSAKDALVVKILRSPHAY
                     AKIKDIDTSKAEMVDGVECVLTYKDVPKSRFTLAGQTYPEPSPYDRLILEDVVRYVGD
                     EVAIVAAIDEKTAIKAMNLIKVKYEVLTPVLDFEEAIGNKTMVHDEDRHCNFDIGMQR
                     EKNIVSHHDYTKGDVEKVLSNCDVVIEETYYTQAQAHAMMETYRAYNYLDHMGRLVVV
                     SSTQIPFHVKRHLSRALEIPASKIRVIKPRIGGGFGGKQTACVEIFSAIVTLKTGKPA
                     KIIYDRKETFNCSTSRHAMRLKVRLGATNDGIIKAIDIKALSDTGAYGEHASTTIGLV
                     GEKTLPMYNKVEATRFIADVVYTNKMPAGALRGYGASQGCFAVESTINMLANKLKMDP
                     TDLRLKNVVTEGETTFAYGKTLNSVDLIKCINKGKELIGWNEKYPCKHLDNGKIRSVG
                     MAITMQGSGIQGLDKSSAEIKLNDCGDYTLMIGSTDMGTGSDTILAQMAAEVLDTSIE
                     NITVISADTDLVPYDPGSYASSTTYVTGMAVVKAAQDLREKIISAGATKLGVEKDEVE
                     FDGIKLTSKKGSTLLFDIAVDSTIGPDTNQLIGLASHGSPVSPPPFIAGFAETEIDTE
                     TGKVDLVNYVAVVDCGTVINKNLAKIQVEGGIVQGIGMALFEEVRYTEKGSMDTNTFM
                     QYKIPARCDIGNVKVDFVETYEPTGPFGAKSVGEVVINTPLPAIQEAVFNGIGVRINT
                     LPITPEKVFMEMNK"
     gene            12432..12743
                     /locus_tag="PC414_002002"
     CDS             12432..12743
                     /locus_tag="PC414_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464397.1"
                     /translation="MAGNLNNMRAVNNFRGDKNILECLVSFEGRSISQRKVRVFFKEK
                     QNQIEIDFAEEEISKLVENVVLNTSYQEMLYDEIEKQLEIDCIGTWMILSKLKDGSRV
                     H"
     gene            13029..13529
                     /locus_tag="PC414_002003"
     CDS             13029..13529
                     /locus_tag="PC414_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HDF2464398.1"
                     /translation="MRKNSINIALTAAITAIYAVLTLSLGFISYGPIQFRVAEIMMLL
                     AFLDKGYIVGLTLGCFLANVIGPYGVPDIIFGTLATFISASMIYITRKLGKQNKSTLV
                     IASLWPTVINSIVVGLMLNVFFGLPLILSMIQVGFGEFVVVTIVGVPFFCFLMKKYKN
                     VINIKF"
     gene            13586..14572
                     /locus_tag="PC414_002004"
     CDS             13586..14572
                     /locus_tag="PC414_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HDF2464399.1"
                     /translation="MSKVKRLTDVWIYINDKQKFTIKELSEEFNLSAKTIQRYLKELN
                     KMGLPIQAEQGRNGGYRVLNNSYIPPVIFTEKEVMIILFALKSLQIYNYKLMEIEINS
                     IMRKVSYERKSSIKVGIENIKRYIGFVANDTNDGDFKSSNVTEFFRASSESLILNIKY
                     KCGNQILEKNVCPIGIYLNKGAWFSPAYDCKSDKIILVHIDNIIDIKRTGESKKIHIN
                     LEEWLHEVYNNLYGNSSTTKKFGEIIYLNVLLTKEGVSKIKDSSWNLDMELNEDGSGI
                     ISTFIKVEDIDCYASILFFIGNNAKVIEPKFLNKLLYDKANELRYFYEENMV"
     gene            14622..15344
                     /locus_tag="PC414_002005"
     CDS             14622..15344
                     /locus_tag="PC414_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HDF2464400.1"
                     /translation="MENFNKNAKIIGIGAEGINIINEVEEKIKANMDIEKININQEIE
                     KEYVRSLLDGVDILFLIYSSEDKHIRDIIKAVSYMSNERRVLSIGMDCSEKENKEDLE
                     LGREFKINNDSIFKFVDLMNIMVESISDSCMINIDITDLKEAIVGDKGIKYSFEEFED
                     TKSYSEIADILFDRMEYIGEEFISKKGIVFVEGSPEFSIMELNDLISNIQSKVEESYE
                     VIFSLYIKENLNGNIRVGLLYN"
     gene            15373..17307
                     /locus_tag="PC414_002006"
     CDS             15373..17307
                     /locus_tag="PC414_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2464401.1"
                     /translation="MSENIRGTEYWLMEEFFYQETDTPQIFREESIIALGFDIKINLK
                     KVFSMGKKSEKYINENCNDKDFIKKFMSIKKGDYFLYKAYQNIEEKVKCNVSIGIVEE
                     DFENGYELSHILGHTLPVKWMHNFDTVSNCKVYTSEFYKLNNNDISSYLEIIKSQDKK
                     LLRKRIEESENNKWYLKNYYYKNVDKCVDDEELFIQPINLEQLESLKNIKTDDYILLY
                     NSEKVICENDHTMKSLFGVCQVMEGFDKEKVSALEGGYFFPVRWIEYEKENIDGNAWV
                     IFENNSIVEKYVKRFTNKMPLNTILYGPPGTGKTYNTKQMVYNLVSETSLRNRYYTGY
                     LDYKNNVEFCTFHQSYGYEEFIEGLKSDGEGNFKPEDGILKDISIEACYNALKFEKKM
                     SFELEKDSLTLETIKDRKKKLVLENIEYSESFNFEESENYVLIIDEINRGNVSKIFGE
                     LITLLEDDKRLTKSNQTIVKLPYSKEKFSLPPNLYIVGTMNTSDKSIALLDIALRRRF
                     NFEEIMPDYDLLSIVDGIDIRKMLYTINKRIEFLYDRDHVIGQAYLLAVENINDIVVV
                     FRSKIIPLLQEYFYYDSEKVGLILGGIGRNEKDKYIVYKEKLIASELFKNNNASIMES
                     KVNYCIKQNISSEELKNIYE"
     gene            17300..18568
                     /locus_tag="PC414_002007"
     CDS             17300..18568
                     /locus_tag="PC414_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HDF2464402.1"
                     /translation="MSKNIYVKETYEWIRIGNGENELTEIEYEKLLKYLENNNDVLKS
                     NIIDIKYKKLRFINYVGIICFENVILEILPKLSLSDNLVKDREILLQMLSICNKIPIT
                     MNEKIRLSLKNYNLLNFFVMYFIESMQTQMKKGIYFEYINKIENLNVMRGKILLSTYA
                     KEKGISPMKIRCEYDEYSENNFLNQVLKKACISILCRINDNSIQGKIKKILSYFQNVD
                     LIYIDRKKLLDYKFYKNNDRFKDCYLLARLLLLNLSMDNSQNNQEAFSILFEINTLYE
                     EYIGILIKSIWDNSFRETYIQDKSKFLLKNEQTGKKNFNLRPDIVLYDLKNEYEIIID
                     TKWKAIEVDSNVFYRSSDIYQMYAYITAYENAKRCILLYPCIQKDKNYSSWKLSESFK
                     GKFIEAKTVRLDDIKNTKNDLKKIIFNYKF"
     gene            18895..19935
                     /locus_tag="PC414_002008"
     CDS             18895..19935
                     /locus_tag="PC414_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2464403.1"
                     /translation="MDKHSDSVMYGGMEVYNIWGTTTVDSIIGESDVMRALKNRICKI
                     GNSDSTVAITGESGTGKELIARAIHLAGNRGNKTFVAINCAAIPEPLLESELFGYTKG
                     AFSGADSKGKIGKFELANGGVVFLDEIGDMPIQLQSKLLRVLQEKKFTRIGSNELIDI
                     DVRIISATNRDLFELVKENKFREDLYYRLNVIPLEVPPLRERGDDIQILINNFIDKYS
                     RKMKKNVYHIEPDVEQMLLKYPWPGNIRELENTIELALNLLEEDGIIHKGIINRKILE
                     YFKLNNINIKLLEESDYEISMEQDILTLEEFERAYIKKVLKFYGNDTKSKKLAAKKLG
                     ISLATLYRKIDV"
     gene            20291..21613
                     /locus_tag="PC414_002009"
     CDS             20291..21613
                     /locus_tag="PC414_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HDF2464404.1"
                     /translation="MFDIDGRPPILKCLPLSMQHILAMIVGTVTVPIVVGGAVGVSPT
                     QITLLVQYALIIAGISTLIQLYPIGNIGSRLPIIFGVGFTYVPTLVAVGGNYGLASIL
                     GAQLIGGVATVLIGIFIKKIRKFFPNIVAGTVVFTIGLSLYPIAINYMAGGVNSPTYA
                     SMQNWAVAAITLVVVIGLSQFAKGYAKLAAIFLGIIVGYCVSLVLGIINFDPIREATW
                     FAIPKPLEFGMEFDMPVIISVVIVSIINAVQAIGDLSATTMGGMDRNITDKELSSGVI
                     GSGICTMIGALFGGLPPSSYSQNVGMVAMNKVISRFVLGIAGIFMLLSGFVPKFGALM
                     TTIPYSVLGGATISIFSMITMTGIKLIITEELSSRNITIVGLAIALGMGITSVPAAQE
                     AFPSFVKLVFGESPIVIATLVAFVLNLIIPNKSLEDEAKERAVMEETN"
     gene            21910..23271
                     /locus_tag="PC414_002010"
     CDS             21910..23271
                     /locus_tag="PC414_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HDF2464405.1"
                     /translation="MKTRNTSKYDVDGIPPLKESIPLALQHLLAMIVGNMVPAILIAN
                     VVGLNQGQATMLIQGSMLAAGLATFLQLYPIPLFKGFKLGSRLPVMMGMSYVFLGACL
                     SVAAKDGLAALFGAQIAAGVIVFFVGFGVKKIRHIFTPIVSGTIIACMGLGLFATAIK
                     NLAGGEGTQTFGSPINFTVGVIVAFVIIMINKYGKGLVKNSSILIGILVGYAISLVLG
                     LVDFSAVQGAAIVSLPTPAAFGLEFRPELIVMFTIIYIIGIADMMGACTIATVGAMDR
                     EVTDEELASVVLGNSITSIISSLFAALPTGVFSQNTVIVSMNKVTSRFVIALGALVLL
                     LAGISPALGAIMTTIPSCVVGGATLVVFSSIAMSGFSIMSMDGFTEENNLIAGVSIAT
                     SMGLTTAPQVLDQFPETIGTVLGGSSIVSGAIIAILLQTLFKLKSRKSAENVTSNLEE
                     NIG"
     gene            23344..24738
                     /locus_tag="PC414_002011"
     CDS             23344..24738
                     /locus_tag="PC414_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2464406.1"
                     /translation="MIDILIKNAIIVTVNKDRDVIFDGAIAIQGDRILDIGNTVDIEP
                     KYLDAKKVIDANGKVIFPGFINTHNHLFQVLLKGLGDDMALHEWLNTMMFPSAKFLTE
                     QDTYDAAMLGCMEGLKSGITTMVDYMHTHNRPGLTDGIVKAYKDLGIRGVVGRGCIDL
                     GIHKELIEDVETVEKDLRRVFEKYHNSENGRIKICVAPSSMWAISEEMGKMLWNIVKE
                     YDSYFTVHISETEFARTATKDIHGEIDIKLLEKWGIVGPEVVAVHCVCITDEDMEMLK
                     KYDIKVSHNVASNMYLASGVAPVPEMLKKGINVSLGLDGAASNNAQDMVELMKLTALQ
                     HKVNNCDPLAISAEKVLEMATIEGARTLRMEDEIGSLEIGKKADLVIFNSMLSPKSIP
                     LHNPVSTLVYSASMHNVESVLVDGNVILENGKVTTIESEEQVLLNAQHAAEELCKRAN
                     VTNRMEGHKWNSLY"
     gene            24800..25270
                     /locus_tag="PC414_002012"
     CDS             24800..25270
                     /locus_tag="PC414_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HDF2464407.1"
                     /translation="MEKIKLNINDYMYEIEVEKNETLLHVLREKLNLTGAKQGCNSGS
                     CGACKVLIDGVDMKSCKLLAKNCEGKVIYTIESFANGENLHPIQEAFIEVGAVQCGYC
                     TPGMIITTQALLNRNASPNEDEIREAFKENLCRCTGYVKIIEAVKLSAARLGGR"
     gene            25273..27489
                     /locus_tag="PC414_002013"
     CDS             25273..27489
                     /locus_tag="PC414_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF2464408.1"
                     /translation="MEKVIGKSYPLKDAALKVTGQMKYVADMKPQNVLYAKMLLSPVA
                     HAKIKSIDTSEAEKVVGVRAIATYLNTSQRKYNGSLRFYEHKIPENEVIFSDTVRFVG
                     DRVAAVAAETPEIAEKAVKLIKVEYEELPAVFTIQDAIKEDAICIHGDSNIVSQNNIN
                     GGDVEKGFKECDYIFEDEYSTPAIHHSAIETHSVIAHYDYNDKLTVHTPCQNTFGFRI
                     ILSQIFGMSLNKVRVIRPAIGGSFGGKLELTIEPVAAALSKMTRRPVKMVLTRKESMI
                     STRTRHGSFVKLKTGVNKDGEVIAQDIKIYTNTGAYASSALNVIGALSHKVFKVYKIP
                     NIKFTGMPVYTNTPIAGAMRGYGSPQIFMAQQAQFAKIAKEIGMDLVDFQNKNAVEPD
                     DVDIIFHGSLGNPRVLDCIEQGKKMFKWDEKKKLPKEEGKYLRGIGMAIGAHGNGVFG
                     AHRDVVTLTLKLNEDGTLTLLTGAHDMGNGVVTMQTMMVAEVLGITPDKVDTFETDTD
                     ACTFNLGDYASRGVFVEGGGAKKTAEKLKNLILEEAEKLLETSKEDLYLDNGCVISKL
                     DENVKASLSDVAVYSQSKSLRELTVVEDYSSPAGRTSYGVHFAEVLVDKETKDIKVVD
                     FVAVHDVGRVINPLNLEGQLEGGIHMGLGYALSEELAFDEQGRPKATDFKTYKLFRTV
                     DMPKCQVAFVDGYEKAGPFGGKSIGECAVVPVAPAVANAVYNATDVEIHSLPIKLK"
     gene            27572..28243
                     /locus_tag="PC414_002014"
     CDS             27572..28243
                     /locus_tag="PC414_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2464409.1"
                     /translation="MAKILGICGSPRKGATEYAVLEALKEAETIPGIETEYWSVRGKK
                     ISPCVHCDACIRKETMCIIKDDIQELEQKILEADGIIVGSPVYDMNITAQLTAVFNRL
                     RPMYLVHPGKLQNKVGAAITTGGTRYGGQELTKLPIINFFLMHEMLVSGGLGGCYIGG
                     TIWSKDGKAKGAQEDETGMDTVKRLGKGAAEAVMVSKFGLEKWNVVKEELDVKKDEKS
                     PLRDH"
     gene            28606..29820
                     /gene="dpaL"
                     /locus_tag="PC414_002015"
     CDS             28606..29820
                     /gene="dpaL"
                     /locus_tag="PC414_002015"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HDF2464410.1"
                     /translation="MLKEIKWKVNNLPKGDKENCIKFLNEEEITKVRNFHKSFPQYKE
                     TPLANLEGLAKKLGVAGVYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLDTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAQEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNHAAAFVSAPDWVSAKGMRTLGNP
                     LNGDEKVISGESGAVTTGLLVAAMEREDLADLRKDLKLDENSRILLISTEGDTDPDKY
                     RSIVWDGEYPSI"
     gene            29947..>30537
                     /locus_tag="PC414_002016"
     CDS             29947..>30537
                     /locus_tag="PC414_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDF2464411.1"
                     /translation="MLNEQRKQEVTEICQKLIQNPSSSGNEEGVVKAIEESFEKLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEGKIVDGKIYGRG
                     TSDMKGQYTAMMSAVAYFAKDTNRDFAGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKG"
BASE COUNT        11644 a         3190 c         5578 g        10125 t
ORIGIN      
        1 cgtatatatt gacaaaatta attatataaa ttaaaataat attcgtaaag aaaaattcat
       61 gttttgtact gtataacata taattttgac aatatgggtc ataagtttct accggaatac
      121 cgtaaatatt ctgactatgt atactattat ttagttaatt aaatatttaa ttaactgaat
      181 tatgaaggat acggctcagc ttatcaacta ctgtgtcgtt tttttgtaaa atttaatagg
      241 aggtgttgtt atgcaaacaa caaacagtag tactatgcag aaactttttc caattttgac
      301 taataaaaat gtcgacatga agaaggaaat aattgcagga gtaactactt ttttaactat
      361 ggcttatata atagctgtaa atccaaatat tttatctgag acaggcatgc ctgcaggtgc
      421 acttgttaca ggtacttgtt tagcagcagc atttggatgt attttaatgg gtgtagttgc
      481 taaccttcct tttgctttag catcaggtat gggtcttaat gcattttttg catatacagt
      541 agttttacaa atgggtgttc cttgggaagt agctcttaca gcagtatttg tagaaggtat
      601 tatttttata gtattatcag taagtggagt tcgtgaggct gttgtaaatg cgataccaaa
      661 aaatatgaaa ttagctgtta caggtggtat aggaatattt attgcactta taggtctagt
      721 taacagtgga attgttattg gagaccaagc cactcttatc aaaatgggta gatttacacc
      781 agcagtaata ataacttgtg taggattaat aattattgct gtattagata aaaaaagagt
      841 taaaggttct atactttttg gaatagtagt gagttcatta cttgcttggg catttgcttt
      901 tataaatcca gagtatgcac aaaaattagg tatatattta cctggtggat tatttaaatt
      961 cgaaagttta gcaccaatag caggtaaaat agatttagga tatgtactac atccaacaaa
     1021 tataggtggt tttttagtag tagtttgtac atttttattt gttgattttt ttgatacagt
     1081 aggtacatta gttggtgttt gttctaaagc aaacatgtta gatgaaaaag ggaatgttcc
     1141 taatgtaggt agagccttac ttacagatgc ggttgcaact acagttggtg caggacttgg
     1201 agtatctaca gttacaactt atgttgagag ttcaacagga gttatcgcag gtggaagaac
     1261 tggatggaca gcaataacag ttggaatttt attcttagct gcaatgtttt tctctcctgt
     1321 atttatagca attccatcat gtgctacagc accagcttta atatatgttg gatatttaat
     1381 gcttggtaca gttaaggata tagagtttga taatataact gaaggtgttc cagcttttgt
     1441 tactattgct tgtatggcat taacatatag tataggtgat ggtttaactt taggtatatt
     1501 aacttatgtt ttcgtaaata tattttataa tatatttgga gctaaaaagg cagaagacaa
     1561 aaaacatgtt tctgtagtaa tgattgttct ggcaatatta tttataataa aactttggct
     1621 actataatag gtattattta aaattttaat ttaatataaa ataaagggct gtaatttatt
     1681 atcaattgtg atgataaact acagctcttt tgtatttgtt gtatttttgt atttattgta
     1741 ttatttaata atgtctatta gttctccatt ttgtaaagtt ttaggtgtaa ttactatatt
     1801 gaactctttt tgcaaatctt ttataatatc taattctttt tttgttatta ttgagattgt
     1861 gttaccactt cgatttcctc tagcagttcg accacatcta tgcaggtatt catttttact
     1921 ttttggaaaa tccaagttaa aaacatgact tacatccaca atatctagtc cacgagcaga
     1981 taaatcagtt gtaattaaaa tctttgcttt accaagttta aatttattta ttgcattctt
     2041 tctatcttct ttttccatat ttccaaaaat tcctattgct ttgtaattat gataatttag
     2101 ttttgatact aggacttcta tatttttttc attatttaca aatacaatag ctttttttgg
     2161 atttgtagct gctaatgcct ttctaagaaa agtaaattta tcccttatct cacctaataa
     2221 atatgagtga ttgatgttag gatttatttg tgatttttcc tttgttttta ttatttcaaa
     2281 ctccttcata agtttatcac aaatttttat agtgctatca gtaagacttg cagagcatcc
     2341 tataatttgt ctatctctta acgttgttct tattatgtct tctatacaag ttatattttt
     2401 tccattcaat agattgtcca cttcatctaa gataatagtt tttatattat gtgattttag
     2461 tttcttttgt tttatcaagt ctaaaactct tccacaagag ccaattacta tgtgtggttt
     2521 tactgctttt atatttttta tttgtttttg tatattgacc tccccaatta aagctaaaga
     2581 agtaacggag agttctgcat ttttagccaa taattcaacc tggtttgtaa tctgcattac
     2641 taattcatgc gtaggtgcaa gtataagagc ttgtgtttct ctttttgatg tatcaatttt
     2701 ttcaaatata ggtaaaaggt atgctagtgt ttttcctgtt ccagtctgtg aattaatcaa
     2761 taaatcctta ttttgaatta tattccctat aacaagtgac tgaacttctg ttggagaagt
     2821 tatgtcttgt tttttgagtc catctattag agtggaactt atttttaatt gttcaaatgt
     2881 attcatattt tactccttat attttaataa ttatagtcaa ctatttttta ttatacattg
     2941 tttattcaaa tcgaactata tattctttta taattatgtg taatttgaaa gtaaatatca
     3001 attatattta tacctttata tttctgatac ctttgcattt tatagatttc taaaataaac
     3061 aaataaaagt tgtaattgtt tgatatttgt tatatattat atataacaaa tattaaaact
     3121 ataatatgga gggaaattat gttaaaagta atcaatgtat ctaaatcata taaaaataaa
     3181 aaagtagtaa gtagtgtctc atttgatgta aatgatggag agctttttgg gtttatagga
     3241 cataatggag caggtaaaac tactactatt aaagcaattg ttggaataca cgattttgaa
     3301 gagggagagg tactaataaa ttctaaatct ataaaaaaac agcctgttga atgtaaaaag
     3361 gaaatggcat atatacctga taatccagat ttatacgagt cattaactgg tatacaatac
     3421 cttaatttta tagctgacat atttgaagtt gccaaagata aaagagaaga gttaataaaa
     3481 tattattcaa ataagtttga actcaataaa gcattaggag atttaatatc ttcatattca
     3541 catggtatga aacaaaaatt agctataatt tcggctttga tacacagccc aaaaatctta
     3601 attcttgatg aaccttttgt aggtcttgac ccaaaagctt cctttatatt gaaggaaatt
     3661 atgagggagt tttgtgacaa aggtggttgt atatttttct caacacacgt tttagaagtt
     3721 gctgaaaaaa tttgtgataa gattgccata ataaaagatg gaaatataat tgcttatgga
     3781 actacagaag aagttaaggg aagtaattca cttgaaagta tctttatgga attaatagag
     3841 aaataaaatg ttttttatac aatatagtgt ctaaagtgag gaatgataaa atatgagcaa
     3901 tctaagtata ttaataaaaa ctaatataat aaatgaattt aaattaaatg cgttaaaaaa
     3961 agctgatggt agagaaaaac aaaaaataat gggaatgatg ctttcagttg ttataatagc
     4021 gggtctatta attttttatg taacaagctt aagtctctct ttagttgata tcttaaagca
     4081 aataaatcaa ctggaaatgc tactgatatt tggttttgga ttatcaacat taacaactat
     4141 tgttacatca ttatataaaa cgtcatcata tctatttcaa gcaaaagatt tagacctttt
     4201 gactagttta cctataaaag aatctactgt actagcaagt aagatattaa tgttggttgg
     4261 agcaaactat ttattttctt caatatgtat gattatacct gcagtggttt actttttaaa
     4321 ttctgatgta agtataactt atttccctta tttgatattg ctatttttag ctttaccatt
     4381 attaccgata gttgcttcat caataatttt tctatttata gggcagatat catcaagaat
     4441 aaaatacaaa aatttagtat taatcatagg tagcattata ttgatgttgc tttataccct
     4501 acttatgtca agacttaatt ctatgtcagt tgaaatgcta aagaatagtg tgtctatttc
     4561 agagacaatt agaaaagtat atcctcctat atattatttt gttgatgctc ttaaaacagg
     4621 aagtatagtt tcatttttag catttatagc catctcaata ataccatttt ttatgtttgt
     4681 aactctattt gcaaaaaaat ttaaagatat aaatgctaag atgagtgaag gatataagtc
     4741 aaaaaattat gaatttaagg gtcaaaaaac ttcaagacct tcaaaagctt tattaaaaaa
     4801 agaatttaag agatatactt cttcgtttat atacttttta aattcatctg caggtttgtt
     4861 atttctaatg gtaggtacaa ttttaatatt attatttgga gcagataaag tgggtgagtt
     4921 actgaaattg aatatagatt ttaatcttat aaaaatacaa ttattgggag tattcttctt
     4981 tataatagta atgaattgta caacatactg ttcaatttct cttgaaggaa aaaacttatg
     5041 gatactgaaa tcttctccaa tagatgaaag agaaatattc tggtcaaaaa tattgatgaa
     5101 ttttttattg aatgcaccac tatctatttt atgtctaata ttagtatcta taagattaga
     5161 ctttgaaatt cagtttatta ttgtatcaat ctgtttgatt gtaagttttt caatgtttgt
     5221 agcattgatg ggattattat caaatttatt atacccaaac cttgattgga aaaatgaggt
     5281 ggctgtagta aaaagaagtg caagtatgat agttacaatg ttagtatcat tagcttatgt
     5341 aattatactt ggaggtatat atgtattact tggaatttcg tttttaaata tatatattat
     5401 tttggcaagt atacttactt tgatattaga ttttatgata tggagaatta tttctactaa
     5461 aggagtaaga atatttaaaa atttataggt gtatttatgt caaataaaga cttatctttt
     5521 ttagtcttgc aaataatagg ttaagtagta tactatgtat agtgatatga aaaaatttag
     5581 gagaaaataa atgagaaaat tatccaaaag agaaaaagat tttattcaat atggattttt
     5641 attatttttg atatgtatta ctacttattt agtattaact actttagata taaaactaat
     5701 acctaaaatt ataaggctag taaataagac ttatatattt cttggcatat tgacaattat
     5761 agtatatatg ataattgaat ctattgtaac tcatttgata ataaatgcag ttcaaaaaac
     5821 aaaagtaaaa tttattggta ttaaaatggc tacaatggga ttttactata acttagttac
     5881 tccatttgca tctgggagtc aaccaatgca aatatatgca cttacaaaat atgatgtaag
     5941 ccttagtaaa tcagtggcta tagttactaa taaaactgta gtatttcaaa gtacagttac
     6001 tgtattttgt ggaattttaa ttttgctaaa taagtcatta ctgatggagc aagtacattc
     6061 tgtgaaagtc ttgataactt ttggaatgat tatgaatgta agtatgttgt taggtggact
     6121 actaatagta tttagtccaa aacaagtaaa aatgatagca gaagttttga ttaatttttt
     6181 atctaaattt aaaacattta aatttttaga ttcaaaacgt gatagaataa atcattatat
     6241 tgatgattat aattattcta taaaaatatt tattaaaaat gtaagaatct tagttttaag
     6301 tatattactt actgtgttac aattaatcgc atatttcagt atagtttatt gtgtgtataa
     6361 agcctcaaat ttaagaggag tttcatatgt acatataatg acattacagg tctttttata
     6421 tatggctata tcaccaattc caacaccagg aaatgttgga gctaatgagg ttacattttt
     6481 tactatgttc tccaatataa ttccaaaaga attattgggg tactctgtac tcttatatag
     6541 tgggtttgta tactatttta tattaatagt ttcaggttta tttacatttg gaacgcatta
     6601 tacattaaat tcttggaaaa aggaaaataa agtaaattct aatttagata aggttattga
     6661 aaaattaaat taataaaata attagggaaa ttctatatat aaatatggtg ttgaataagc
     6721 tatattatat tgagatattt cccttttttg attaaaatta tagaaatttg aaataataag
     6781 taataaacag aaaaagtgat attgttttga aaggagaaac tatgaaacaa gataaacaaa
     6841 atgaaaaaca aagtgagatt tctaagataa aacaaagcaa tgataataaa cttgaaatag
     6901 ctcaagaatt acatgtaagt aaaaatatag ggaatatgct tggatataga atagataaga
     6961 aaaaaatgaa tagaccaagt taatgaaaat gttgaaaatg gtacatttta aaaaatatat
     7021 ataatgaaat gttttattgt taaatttaaa cttattttaa tgggggaaaa agtcgaaaaa
     7081 tatctaaaaa gatatggata taaaaaatat taatatatat atactataaa cttgacaaaa
     7141 tcaacctttt aataactagc aaaaaatagc aaaattatat aagcgttatt taattgtcaa
     7201 aaatgaaagc taaattgcat gtcctaaaaa aaatgattga taacatttat ttgtgtgcta
     7261 taattatata gtaaaacaat aagtatattg cataatgtat gcaaatcggg aggagagacg
     7321 gacatggaag catctataca cacaattgcc agcaataatt tattgataat ttttgctatt
     7381 gtaagtataa ctggaataat ttgtagtaag cttagtgaaa taataaaggt tccagatgta
     7441 gtgttatatt tacttgtagg tattttaata ggaccatcat ttttaaaatt tatagatatt
     7501 cgtggatttc aaatagaaaa tcaactaata cttacatttg gttctgcatt tattttatat
     7561 ttaggtggaa aagaaataag tttaaaagta ttgagaaatg tgaagatatc tgtattctta
     7621 ttagctactt taggagttgt gatatctgct tttataatgc aacaagtaat aggatttact
     7681 tttgggataa gtgcaatgac tgctttacta gcaggaacta taatagcatc aacagaccca
     7741 gccactcttg tacctatatt taatcaggta aagataaagg atagggtaaa acaaactgta
     7801 ataagtgaat cagcttttaa tgatgcaaca ggagctatac taacatcagc agtgttggct
     7861 gtaattttgt caaataaatt ttcattaggt gaaaatattt atgaactggg gacaatggtt
     7921 attgtcggag ttttagttgg tttaattaca ggaatactgc ttttgaaatt agttaatgat
     7981 aaaccttatg gaatatttaa agattttgca ccaataatat ctgttatttc agttgtagtt
     8041 gcatatgaat tatctacaaa attaggtgga agtggatata tgtcttgctt tattgtaggt
     8101 ataataactg gtaacaagaa aaattttaaa atttggttaa gtcaaaaatc ttatgatgca
     8161 gatttttatg ttgctgaaac tttaggtact atttgtcgta tggcaatatt tataatattg
     8221 ggtagccaag ttaatttggc tcagttaagt aaatatttta tgccatctgt aattacagta
     8281 ttagtattaa tgtttatagc aagaccactt tgcgttttag tttgtacact tgtagacagg
     8341 gaagcaaaat ggagtaaaag tgaaatatta tttatgatgt gggtaaggga aacaggcgta
     8401 ataccagcag cactttgtgg tattatttca gcaatgaaag ttcctggata cgaagtgatt
     8461 tcatctgtag tattcatgac tatactaatt acattagtaa tacaaggaag tactacaaaa
     8521 ttagtagcta aaaaacttgg attattagaa gaagaagtga taaatataag tgaaaaagta
     8581 tcaacttttt aaagttttgc tttaaaatag tacctgttaa tggctatttt aatattgtct
     8641 aatgatgatt attgaaatag tcattttttt atgtataagt aatttacaga atttacatat
     8701 tagacatatt tattatcaaa atttgttgaa gctatgatat aattagttgt atttaaaata
     8761 agaatagcaa ataagggagg aaatttatgt ttacggttat ggatattgta cagccagata
     8821 cagtagaaga agcgtactca attttaaata aaagaaaaac taaccaagta attggtggaa
     8881 gtgctttttt aagaatgggg aaaaaaagaa taggaacagg aatagaatta tctaaattaa
     8941 atttagatta tataaaagag tatgaagatt acgttgagat tggttctatg actactttta
     9001 gaacattaga aacaagttcc ataataaaaa ataattttgg aagaataata gaagattcag
     9061 ttaaaaatat aataggagtt cagtttagaa atgtagtaac agttggtgca acagtatttt
     9121 ctaaatatgg tttttctgac ttgatagtag cattactttc attagatacg gaagtagaat
     9181 tatataatat tgggagaatt agtcttgaag agtttctaaa tagagattat gagaaagatt
     9241 tacttattaa gatatacata aagaagacaa ataagaatgc ttcttataaa tcaattagaa
     9301 atgcaaagag tgattatcca attctaaatg tatctgtatc aaaacatatg gaacaattta
     9361 agttatgtgt aggagcaaga ccgcagaaag ctacgattgc aaaacaagct agtgaatttc
     9421 tatctaacaa tgaaatcaat gaaattaata tagataaagc agtagaaata gcatcagaag
     9481 aactaacttt tggttctaac atgagagcat ctagggagta tagaaaagct atgagtaaag
     9541 tacttttaaa aagagctata atggaggtta tttaatgtta gtaaagatta atataaatgg
     9601 gaagaaaaaa agtgttgata tagaaccaga agaatatctg gttgatactt taagaaaagt
     9661 tggtaattta agtgtgaaaa gaggttgtga cacaggttgt tgtggtttat gtacagtttt
     9721 aattgacaag aaacctacat tatcttgtgc aacactgaca ttgagagctt tagataaaga
     9781 aataactaca attgaaggtt tagaaaatga agttaaagaa tttagtgaag ttttggtaaa
     9841 agaaggagct gagcaatgtg gtttctgttc tcctggcttt atacttactg tacttgcaat
     9901 gaaggatgag cttgaaaatc ctacagatga tgaaataaag cattatctta caggaaatct
     9961 atgtagatgt acagggtata tgggacagtt aagagctatc aaaacttatc tggaggtaaa
    10021 atagaacatg aatatagttg gaaaaggaat caagaaaata gatggaatga gtatcacaac
    10081 aggaaagcct gtatatacag atgatttatc agcaaaagat gctttggtgg taaagatact
    10141 tagaagtcct cacgcttatg caaaaataaa agatatagat acatcaaagg cagaaatggt
    10201 tgatggtgtt gaatgtgttt taacatataa agatgtacca aaatctcgat ttacacttgc
    10261 tggtcaaaca tatccagagc catctccata tgatagatta attcttgaag atgttgtaag
    10321 atatgtaggt gacgaagtag ctatagtggc agctatagat gaaaaaactg caattaaagc
    10381 aatgaatctt atcaaagtaa agtatgaagt acttacacct gttttggatt ttgaagaggc
    10441 aataggaaat aaaactatgg tacatgatga agatagacat tgtaactttg atataggtat
    10501 gcaaagagaa aaaaatatag tatcccatca tgattataca aaaggtgatg tagaaaaagt
    10561 attaagtaat tgtgatgtag tgattgaaga aacttattat actcaagctc aagcacatgc
    10621 aatgatggag acgtataggg catacaatta tttagaccat atgggaagat tggttgtagt
    10681 aagttcaact caaataccat ttcatgtaaa aagacattta tctcgtgcac tagaaatacc
    10741 agctagcaaa attagggtta taaaacctag aattggtggc ggatttggtg gtaaacaaac
    10801 tgcatgtgtt gagatttttt ctgcaattgt tactttaaaa acagggaaac cagctaaaat
    10861 aatttatgat agaaaagaaa cttttaattg ttcaacaagt agacatgcaa tgagattaaa
    10921 agttagacta ggtgctacaa atgatggcat aataaaggca atagatataa aagcactttc
    10981 tgatacagga gcatatggag agcatgcttc tacaactatt ggtttggttg gagaaaaaac
    11041 tttaccaatg tataataaag ttgaagcaac aagatttatt gcagatgtgg tatatactaa
    11101 taagatgcca gcaggagcac ttagagggta tggagcatca caaggatgtt ttgctgttga
    11161 atcaactata aatatgcttg ctaataaatt aaaaatggac ccaacagatt taaggttaaa
    11221 aaatgtggta acagaaggtg aaactacatt tgcatatggt aaaactttaa atagtgtcga
    11281 tttaataaaa tgtataaata aaggcaaaga acttattgga tggaatgaaa aatatccttg
    11341 taaacatctt gataatggaa aaattagaag tgtgggtatg gcaattacta tgcaaggttc
    11401 tggtatacaa ggtcttgata agtcttcagc tgaaataaaa ttgaatgact gtggagatta
    11461 tactcttatg ataggttcta ctgatatggg tacaggaagt gatacaattt tagctcaaat
    11521 ggctgctgag gttcttgata caagcattga aaatataaca gttatatcag ctgatactga
    11581 cttagttcca tatgacccag gttcatatgc ttcaagtact acttatgtaa caggtatggc
    11641 agtagttaag gcagctcaag acttaagaga aaaaataatt tctgctggag caactaaact
    11701 aggtgttgag aaggatgaag tagaatttga tggtataaaa ttaactagta aaaaaggtag
    11761 cactttatta ttcgatatag cagttgattc tacaattggt ccagatacta atcaattaat
    11821 aggtcttgct tctcatggta gcccagtatc tcctccacct tttatagcag gttttgcaga
    11881 aactgaaatt gacacagaaa ctggaaaagt tgatttagta aattatgtag ctgtagtaga
    11941 ttgtggaacg gttataaata aaaatctagc taagatacaa gttgaaggtg gaattgttca
    12001 agggattgga atggcattat ttgaagaagt tagatacaca gaaaaaggaa gcatggacac
    12061 aaatacattt atgcaataca agattccagc tagatgtgat attggaaatg ttaaagtaga
    12121 ttttgttgaa acttatgaac caactggacc atttggtgct aaatctgttg gagaagttgt
    12181 tataaataca cctttaccag caatacaaga agctgtgttt aatggaattg gtgtaagaat
    12241 aaatacactc cctattacac ctgaaaaagt ttttatggaa atgaataaat aattatttat
    12301 gaaaatttag actagtttta catgttaaac aaccagttat gtcatacctg ttgaaagata
    12361 tttaataagt agttattata atattatgaa ttttacttat aaaatacaaa attaaataag
    12421 gggatgatgt tttggctggt aatctaaata atatgagagc agtaaataat tttagaggag
    12481 ataagaacat tttagaatgt ttagtcagct ttgagggtcg ttcaataagt cagagaaaag
    12541 taagggtatt ttttaaagaa aaacaaaatc aaatagaaat tgattttgca gaagaggaaa
    12601 tttctaaatt ggttgaaaat gttgttttaa atacatcata tcaagaaatg ttatatgatg
    12661 aaatagagaa acaactggaa attgattgta taggtacttg gatgatatta tctaaattaa
    12721 aagatggtag tagagttcac tgattttaaa ataaattatt agagcctaat tttaaggctc
    12781 ttttttgctg tttttaatta acaatagaaa atttatcttt agtttgtata taattttaat
    12841 atcatatatt ctatatatat aaaattgaat ttgtatgtat tatattgtat aatataaaat
    12901 gaattgggaa aatagttcgc taaaataatt tgtttttata aaattactgt ttaagtttat
    12961 agattttgta taaaataaag ttatgttatt aattttttta aaaataaaac ttttactagg
    13021 agacagatat gagaaaaaat agtattaata tagcactaac tgcagcaatt acagcaatct
    13081 atgcagtttt aacattatca ttgggattta taagttatgg accaatacaa tttagggtag
    13141 cagagattat gatgcttttg gcatttttag ataaaggata tatagtaggt cttacattag
    13201 gttgtttttt agcaaatgta ataggaccat atggtgtacc tgatataatt tttggaacat
    13261 tagccacatt tataagtgca agtatgattt atattactag aaagttaggt aaacaaaata
    13321 aaagtacttt agttatagct tctttatggc caactgttat aaattcgatt gtggttggtc
    13381 taatgttaaa tgtattcttt gggcttccat taattctatc tatgatacag gtaggttttg
    13441 gagaatttgt agtagtaaca attgtaggag taccattttt ctgcttttta atgaaaaaat
    13501 ataaaaatgt cattaatatt aaattttaaa tagtgtatag taatttaatt aattgagagt
    13561 attattagtg aaaagaggtg tttatttgtc gaaggtaaaa agattgactg atgtttggat
    13621 atatattaat gataagcaaa agtttacaat aaaagagttg tcagaggaat ttaatctttc
    13681 agctaaaaca atacaacgat atttaaaaga attaaataaa atgggacttc caattcaagc
    13741 agaacaaggt agaaatggag gttatagggt gctaaataat agctatatac ctcctgttat
    13801 atttacagaa aaagaagtta tgattatact atttgcactt aagtcacttc agatttataa
    13861 ttataagtta atggaaatag aaattaattc tataatgaga aaagtaagtt atgaacgtaa
    13921 aagcagtata aaagtaggta tagaaaatat taaaagatac attggctttg tagctaatga
    13981 tactaatgat ggagatttta agagtagtaa tgtaactgag ttttttagag cttcttcaga
    14041 atctctaatt ttaaatatta agtataagtg tggtaatcag attttggaaa aaaacgtatg
    14101 ccctataggt atatatttaa ataagggagc atggttttca ccagcatatg attgtaaaag
    14161 tgataaaata atattagttc atatagataa tataattgat ataaaaagaa caggagaaag
    14221 taaaaaaata catataaact tggaagaatg gttacatgaa gtatataaca atttatatgg
    14281 aaatagttca actactaaaa aatttggtga aattatttat ctaaatgtat tattaactaa
    14341 agaaggtgtt tctaaaataa aggacagttc ttggaattta gatatggagc taaatgaaga
    14401 tggtagtgga ataattagta cattcataaa ggttgaagat atagattgtt atgcttcgat
    14461 tttatttttt attggaaata atgctaaggt tatagagcca aaatttttga ataagctact
    14521 ttatgataag gccaatgagt taagatattt ttatgaagaa aatatggtat aatatttaga
    14581 aatgttgaaa ttaaataatt attaaggaga tatacgttat aatggaaaat tttaataaga
    14641 atgctaaaat aattggtatt ggagctgaag gtattaatat aataaatgaa gtagaagaaa
    14701 aaataaaagc aaatatggat atagaaaaaa taaacataaa tcaagagatt gaaaaagaat
    14761 atgttagaag tcttttagat ggtgtagata tattattttt gatatattca tctgaagata
    14821 aacatattag agatattata aaagctgttt catatatgtc aaacgaaaga agagttctat
    14881 ctataggaat ggattgttca gagaaggaaa ataaagaaga tttagagctt gggagagagt
    14941 ttaaaatcaa taatgatagt atatttaaat ttgttgattt aatgaatatc atggtagagt
    15001 ctatttctga ttcttgcatg ataaatatag atattactga tttaaaagaa gctattgtag
    15061 gagataaagg aattaagtat tcttttgaag agtttgaaga tacaaagtca tatagcgaga
    15121 tagctgacat tttatttgac aggatggaat atattggtga ggaatttata agtaagaaag
    15181 gaattgtatt tgttgaagga agtccagaat tttctataat ggaattgaat gatttaataa
    15241 gtaatattca gagcaaggta gaagaaagct atgaagtaat attttcttta tatataaagg
    15301 aaaatttaaa tggaaatatt agagtaggct tattgtataa ttagtatatt ttagatatta
    15361 aggagagtgt atatgtctga aaatataaga ggtacagagt attggttaat ggaagaattt
    15421 ttttatcagg aaacagacac accacagatt tttagagaag aaagcataat tgcacttgga
    15481 tttgatataa aaataaattt aaaaaaagta ttcagtatgg ggaaaaaatc agaaaagtat
    15541 attaatgaga attgtaatga taaagatttt ataaaaaagt ttatgtcgat taaaaaagga
    15601 gattattttt tatataaagc atatcaaaat atagaagaaa aagttaaatg taatgtgtct
    15661 attggcatag ttgaagaaga ttttgaaaat ggatatgaat taagtcatat tttagggcat
    15721 acactaccag taaaatggat gcataatttt gatacagttt caaattgtaa agtatataca
    15781 agtgaatttt ataaattaaa taataatgat attagttcat atttagaaat tattaagtca
    15841 caagataaga aattattgag aaaaagaata gaagaaagtg aaaataataa atggtattta
    15901 aaaaactatt attataaaaa tgtagataag tgtgtagatg atgaagaatt atttattcag
    15961 cccattaatc tagaacaatt agaaagccta aaaaatataa agacagatga ttatatttta
    16021 ttgtataata gcgagaaagt aatttgtgaa aatgaccata caatgaagag tctatttggc
    16081 gtatgtcaag ttatggaagg gttcgataaa gagaaagtaa gtgctttgga gggtggatat
    16141 ttttttcctg ttagatggat agagtatgaa aaggagaata tagacggaaa tgcatgggtt
    16201 atatttgaaa ataatagtat agttgaaaag tatgtaaaaa gatttactaa taaaatgcca
    16261 ttaaatacta tactttatgg accaccagga actggtaaaa catacaatac aaaacagatg
    16321 gtttacaatc tagtaagtga aaccagtcta agaaacagat actatacagg ttacttagat
    16381 tataaaaata atgtagaatt ttgtacattt catcaatcat atggatatga ggaatttata
    16441 gagggcctta agtctgatgg tgaaggcaat tttaagccag aagatgggat attaaaggat
    16501 atatctatag aagcatgtta taatgcattg aaatttgaaa aaaagatgag ctttgagttg
    16561 gaaaaagata gcttgacttt agaaactata aaagacagga aaaagaaatt agtattagaa
    16621 aatatagaat atagtgagag ttttaatttt gaagaatcag aaaactatgt tttaataata
    16681 gatgaaatta atagagggaa tgtatctaaa atatttggtg agctaataac attattggaa
    16741 gatgataaaa gacttactaa atcaaatcaa actattgtca aactaccata ttcaaaggaa
    16801 aaattttctc tacctccaaa tttgtatata gttggaacta tgaacacatc agataaatca
    16861 atagcacttt tagatatagc tttaagaaga aggtttaatt ttgaagaaat tatgccagat
    16921 tatgatttat tgtctattgt agatggtata gatattagaa aaatgctata tacaatcaat
    16981 aaaaggatag agtttttata tgatagagac catgttattg gacaagctta tctacttgct
    17041 gtagaaaata ttaatgatat agtagtagta tttagaagta aaataatacc acttcttcaa
    17101 gagtattttt attatgattc agaaaaagta ggtttgattt taggaggaat tggaagaaat
    17161 gaaaaagata aatacattgt ttataaagaa aaacttatag cttctgagtt atttaaaaat
    17221 aataatgcct caataatgga gagtaaagtt aactattgta taaaacaaaa catatctagt
    17281 gaggagctaa aaaatatata tgagtaaaaa tatatatgtt aaggaaacct atgaatggat
    17341 aaggataggt aatggtgaaa atgagcttac agaaattgaa tatgaaaaat tgcttaagta
    17401 tctagagaac aataatgatg tattaaaaag taatataata gatattaaat acaaaaaact
    17461 tagatttata aactatgtag gcatcatatg ttttgaaaat gtaatacttg aaatacttcc
    17521 taaattgtca ttaagtgata atcttgtaaa agatagagag atactattac agatgctttc
    17581 tatatgtaat aaaattccaa ttacaatgaa tgaaaaaata agattgagtt tgaaaaatta
    17641 taatctctta aatttttttg ttatgtattt cattgagagt atgcaaacac agatgaaaaa
    17701 aggtatttat tttgaatata taaataaaat agagaactta aatgttatga ggggtaaaat
    17761 attattaagt acatatgcaa aagaaaaagg catttctcca atgaaaataa gatgtgaata
    17821 tgatgagtat agtgaaaata atttcttgaa tcaagttctt aaaaaagcat gtatatctat
    17881 tttatgtaga ataaatgaca attcaattca aggcaagatt aaaaaaattt taagctattt
    17941 tcaaaatgta gatttaattt atatagatag aaaaaaactg ttggattata aattttataa
    18001 aaacaatgat agatttaaag attgttattt actagctaga cttctacttt taaatttgtc
    18061 tatggataat agtcaaaata atcaagaggc attttctata ttatttgaaa taaatacttt
    18121 gtatgaagaa tatataggaa ttttaatcaa aagtatatgg gacaattcat ttagagaaac
    18181 atatatacaa gataagagta aatttctttt gaaaaatgaa caaactggta agaaaaactt
    18241 taatttaaga ccggatatag ttttatatga cttaaaaaat gaatatgaaa ttataattga
    18301 cactaaatgg aaagctattg aagtagattc aaatgttttt tataggtcaa gtgatatata
    18361 ccagatgtat gcatatataa cagcatatga aaatgcaaaa agatgtatat tattatatcc
    18421 ttgtattcaa aaagataaga attatagttc ctggaaatta tcagaatcat ttaagggtaa
    18481 gtttatagaa gcaaaaactg tgaggttaga tgatataaaa aacacaaaaa atgatttaaa
    18541 aaagataatt tttaattata aattttaaaa ataagaattt taaaatttat aaaagtaatt
    18601 ttatataggt ataaaaatta cgagtagtat aaaaaattta ataagaactt aaattaaaaa
    18661 ttttaaaaat tatttccata taaaatatgg cattgtaatt gcttatttaa tagaggattt
    18721 ttgtaaaatg aagaaagtat atctatcaaa ttaaaactaa gtagatatac tttttattaa
    18781 gttttgtttt aaaataatat tattaaatta taactaagct tttctgttat gatataatta
    18841 aatagataat gtgaatgatt attatataga atacttattt ggagttgata ttttatggac
    18901 aaacacagtg attcagtaat gtatggtgga atggaagtat ataatatttg gggaacaaca
    18961 acagtagact ccataattgg agagtctgat gtaatgcgtg ctcttaagaa tagaatttgt
    19021 aaaattggaa atagtgattc tactgtagct ataactggag aaagtggcac tggaaaagaa
    19081 ttaatagcaa gagccattca tctcgcagga aacagaggga ataaaacatt cgttgctata
    19141 aactgtgctg ctataccaga acctttacta gaaagtgaac tttttggata tacaaaagga
    19201 gcttttagtg gagcagattc aaaaggtaaa attggtaagt ttgaattggc caatggaggg
    19261 gttgtatttt tagatgagat aggagatatg ccaattcaac tacaatcaaa gttattgagg
    19321 gtattacagg aaaagaagtt tacgagaata ggttcaaatg aactaattga tatagatgtt
    19381 agaatcatat cagctactaa tagagattta tttgagttgg ttaaggaaaa taaatttaga
    19441 gaagacttat actatagatt gaatgtaata cctttagaag tgcctccact tagagaaaga
    19501 ggagacgata tacaaatatt aataaacaat tttatagaca aatattcgag aaagatgaag
    19561 aaaaacgtgt accatataga gccagatgtg gaacaaatgt tgcttaagta tccttggcca
    19621 ggaaatatta gagagttgga aaatactata gaactggcat taaaccttct tgaggaagat
    19681 ggaataatac ataaaggaat aataaataga aagatacttg aatattttaa gttaaataat
    19741 ataaatataa agttacttga ggaaagtgac tatgagataa gtatggaaca agacatactt
    19801 acacttgaag agtttgaaag agcctatata aagaaagtgt taaagtttta cggaaatgat
    19861 actaagtcta aaaaactagc tgccaaaaaa cttggaatat cactagctac attgtacaga
    19921 aaaatagatg tataataatt aggcttttta ttctcaattc taaaatttga aattataaaa
    19981 ttgataaaaa acacttggaa aactattttc taagtgtttt tttgtgtaat agcattttat
    20041 tatccaaaaa taaaaaaaat ttaaaagaat ttaaaaaaat aaatgcttta ataaattatc
    20101 aaatactaac aaatataata aaaattatat atgtaaaaca attatgttaa aaaattggca
    20161 ttataattgc ttcatattaa gttgagatga caaaataaaa aaagaaagga tgttgtgtga
    20221 gataataata actgtcatta aaaaatagtt gggagtgata accttgagta aatatcaaaa
    20281 gaatgtttct atgtttgata ttgatggaag gccaccaatt ttaaagtgtt tgccactatc
    20341 aatgcaacat atactagcaa tgatagttgg aactgttaca gtacctatag tcgtaggagg
    20401 agctgtgggt gttagcccta cacaaataac acttttagtg caatatgcat taataatagc
    20461 tggtatatca acacttatac aactatatcc aataggaaat attggttcaa gattgcctat
    20521 aatttttgga gttggtttta cttatgtacc aacacttgta gcagtcggag gaaattatgg
    20581 attagcaagc attttgggtg cacaattaat agggggagtg gctacagtat taatagggat
    20641 attcataaaa aagataagaa aattttttcc gaatatagtt gcaggaacag tggtttttac
    20701 aattggatta tctttgtatc ctatagcaat aaattatatg gcaggtggtg taaatagtcc
    20761 tacttatgcc tctatgcaaa actgggcagt agcggctata acattagtgg tagttatagg
    20821 tcttagtcaa tttgcaaaag gatatgcaaa attggcagca atatttttgg gaattatagt
    20881 tggatattgt gtatctttag ttctgggtat aataaatttt gaccctatta gagaggcaac
    20941 ttggtttgcg atacctaaac cgcttgaatt tgggatggaa tttgatatgc ctgtaataat
    21001 ctcagtagta attgtaagta taatcaatgc tgttcaagca attggagatt tatcagctac
    21061 tacaatggga ggcatggata ggaatatcac agataaggaa ttatcaagtg gagtaatagg
    21121 aagtggaatt tgtactatga taggagcttt gtttggaggt ttaccaccat catcatatag
    21181 ccaaaacgtg gggatggtag ctatgaacaa agtaataagt agatttgtgc ttggaatagc
    21241 tggtatattt atgttattgt cggggtttgt gcctaaattt ggtgcactaa tgactactat
    21301 accatattct gttttaggag gagctacaat atctatattt agtatgataa caatgacagg
    21361 tataaagtta attattacag aagagttgtc atcaagaaat attaccattg ttggtttggc
    21421 aattgcctta ggcatgggaa taacatcagt accagcagcc caagaggcat ttccttcatt
    21481 tgtaaagtta gtatttggag aatctccaat agtaattgca acattggttg cattcgtttt
    21541 aaatctaata ataccaaata aaagtctaga ggatgaggct aaagaaagag ctgttatgga
    21601 agagactaat taagatataa taatttaaaa gaattgttta aattatttag tatgagattg
    21661 ttagattatt tttgatagct attcatttta ataaaaaatt taacaaataa atatatacca
    21721 ggcaattaat gttcatttaa gttgaaaatc ggggagggaa tatttagaaa tttgttaatt
    21781 ttttataaaa atagctcagt ttagctgtgt ttatcggttg agcaataaat tcatggtcat
    21841 aaaaatattt atagaaaata attataaaaa tatttagtca agatatttat gtatgggagg
    21901 atttatttta tgaaaacacg taatacttca aaatatgatg tcgatgggat accaccacta
    21961 aaggaatcaa tacctttggc cttacaacat ctgttagcaa tgatagtagg aaatatggta
    22021 cctgcgatat taatagcaaa cgtggtagga cttaaccagg ggcaagcaac aatgttgatt
    22081 cagggttcta tgttagcagc aggtttagca acgtttttac agttatatcc aataccttta
    22141 tttaaaggat ttaagttagg ttcaagatta cctgtaatga tgggaatgag ttatgtattt
    22201 ttaggagctt gtctttcagt agcagctaaa gatggtttag cagctctatt tggagcacag
    22261 attgctgctg gggtaatcgt attctttgta ggatttggag ttaagaaaat tagacatata
    22321 ttcactccaa tagtttctgg aactataata gcatgtatgg gacttggact attcgcaaca
    22381 gctataaaga atttagctgg aggagaaggc acacaaacat ttggaagccc aataaacttc
    22441 acagtaggag ttatagttgc ttttgtaata ataatgataa ataaatacgg taaaggttta
    22501 gtaaaaaatt cttcaatatt aataggaata ttagtaggtt atgctatatc tttagtttta
    22561 ggattggttg atttctcagc cgttcaagga gcagctatag tatctttacc aacaccagca
    22621 gcttttggat tagaatttag accagagtta atagtaatgt ttactattat ttacataata
    22681 ggtatagctg atatgatggg tgcctgtaca atagctacag taggagcaat ggatagagag
    22741 gtaactgacg aagaattagc atcagttgtt ttaggaaatt ctataacttc tataatttca
    22801 tcactatttg cagctcttcc aactggagta tttagccaaa atacagtaat tgtatctatg
    22861 aacaaagtta caagtagatt tgtaatagca ctaggggcac ttgtattatt actagcaggg
    22921 atatctccag cattgggtgc aataatgaca actataccat cttgtgttgt tggtggagca
    22981 actttagtag ttttctcatc aatagcaatg tctggtttct ctattatgag tatggatgga
    23041 ttcactgaag aaaacaatct tatagcaggt gtatctatag caacaagtat gggtcttaca
    23101 actgctccac aagttttaga ccaattccca gaaacaattg gaactgtcct tggagggtca
    23161 agtatagttt ctggagcaat aatagctata ttacttcaaa cattatttaa attaaagtca
    23221 cgtaaaagtg ctgaaaacgt tacttcaaat cttgaggaaa atattggata aatgtattag
    23281 atataatatg agaaaaccat atggtaaaat gttgtaatga aagtattcaa agggggattt
    23341 aagatgattg atatactaat aaaaaatgca ataattgtta cagttaataa agacagagat
    23401 gttatatttg atggagcaat agctattcaa ggtgacagaa tcttggacat aggaaataca
    23461 gttgatattg agcctaagta tcttgatgct aaaaaagtta tagatgctaa tggaaaagta
    23521 atattccctg gctttataaa tactcataat cacctgttcc aagtgttatt aaaaggttta
    23581 ggtgatgata tggcacttca tgagtggtta aatacaatga tgtttccatc agcaaaattc
    23641 ttaacagaac aagatactta tgatgctgca atgcttggat gtatggaagg tttaaaaagt
    23701 ggtataacta caatggttga ttatatgcat actcacaata gaccaggact tacagatgga
    23761 atagtaaagg catataaaga tttaggtata agaggggttg taggtagagg ttgtatagat
    23821 ttaggtattc acaaagagct tatagaagat gtggaaacgg ttgaaaaaga tttgagaagg
    23881 gtattcgaaa aatatcataa ttcagaaaat ggaagaataa aaatctgtgt agctccttct
    23941 tcaatgtggg ctatatctga agaaatgggt aagatgcttt ggaatatagt taaagaatat
    24001 gattcatatt ttacagttca tatatctgaa acagaatttg caagaacagc aacaaaggat
    24061 atacatggag aaatagatat aaaattactt gaaaaatggg gtatagtagg tccagaagtt
    24121 gtagcagtac attgtgtatg tataactgat gaagacatgg aaatgcttaa aaagtatgac
    24181 ataaaagttt ctcataatgt agcaagtaac atgtatcttg cttctggtgt tgcacctgtg
    24241 ccagaaatgt taaaaaaagg tataaatgtg agtttaggtc ttgacggtgc agcaagtaat
    24301 aacgcacaag atatggtaga gcttatgaaa ttaacagctt tacaacacaa agtaaataat
    24361 tgtgaccctc tggcaatttc tgcagagaaa gttcttgaaa tggctacaat tgagggagca
    24421 agaactctta gaatggaaga tgaaataggt tctttggaga ttgggaaaaa agctgaccta
    24481 gtaatattta actcaatgct ttctccaaaa tcaatacctc ttcacaatcc tgtatcaact
    24541 ttggtatact cagctagtat gcacaatgta gagagtgttt tagttgatgg aaatgtaata
    24601 cttgaaaatg gaaaagtaac tacaattgaa agtgaagaac aagtgttgct taatgcacaa
    24661 catgcagcag aagaattatg taagagagct aatgtaacta atagaatgga aggacataag
    24721 tggaatagct tatactaata ttaaaataag attgtaataa tattctatta caacattagt
    24781 ttatctgtag gaggagaaaa tggaaaaaat aaaattaaat attaatgatt acatgtatga
    24841 aatagaagtt gaaaagaatg aaactctttt gcatgtactt cgtgaaaaat taaatctaac
    24901 tggagcgaaa caaggttgta atagtggcag ttgtggagca tgtaaagttt tgatagatgg
    24961 agttgacatg aaatcttgta aacttcttgc aaaaaactgc gaaggtaaag ttatatacac
    25021 tatagaaagt tttgctaatg gagaaaatct tcatccaata caagaagctt ttatagaagt
    25081 cggagctgta caatgtggat attgtactcc tggtatgatt ataacaacac aagctctttt
    25141 aaatagaaat gcatctccta atgaagatga aattagagaa gcttttaaag aaaatctctg
    25201 tagatgcaca ggatatgtaa agattataga agctgtaaaa ttatctgcag ctagattagg
    25261 gggtagataa ttatggaaaa ggtaatagga aaatcatacc ctctaaaaga tgcagcttta
    25321 aaagttactg gtcaaatgaa atacgtggct gatatgaaac cacaaaatgt actttacgct
    25381 aagatgcttt taagccctgt agcacatgca aaaataaaaa gtatagatac aagtgaagct
    25441 gagaaagtag taggtgttag agctatagct acttatctca atacatcaca aagaaaatac
    25501 aatggttcat taagatttta tgagcacaaa atacctgaaa atgaagttat attttcagat
    25561 acagttagat ttgttggtga ccgtgttgca gcagtagctg cagagactcc agaaatagca
    25621 gagaaagctg taaaacttat aaaagttgaa tatgaagaat tgccagctgt atttactata
    25681 caagatgcaa taaaagaaga tgctatctgc atacatggag attcaaatat agtttctcaa
    25741 aacaacataa atgggggaga tgtagaaaaa ggcttcaagg aatgtgatta tatatttgaa
    25801 gatgaatact ctactccagc aatacatcat agtgcaatag agactcatag tgtaattgca
    25861 cactatgatt ataatgataa attaactgta catacacctt gccaaaatac atttggattt
    25921 agaataatac tatctcaaat ttttgggatg tcattaaata aagtaagagt tataagacct
    25981 gctattggag gctcatttgg tggaaaatta gaacttacaa ttgaaccagt agcagccgca
    26041 ttatcaaaaa tgacaagaag accagttaaa atggttctta caagaaagga atctatgata
    26101 tcaacaagaa ctcgtcatgg gtcttttgta aaattaaaaa ctggtgtaaa taaagatggt
    26161 gaagtaatag cacaagacat aaaaatatat acaaatacag gagcatatgc atcaagtgct
    26221 ttaaatgtta taggagcact tagtcataaa gtatttaaag tttataaaat accaaatata
    26281 aagtttactg ggatgcctgt atatacaaat acaccaatag caggagctat gagaggctat
    26341 gggtcacctc aaatatttat ggctcaacaa gctcaatttg cgaaaatagc aaaggaaata
    26401 ggtatggatt tagtcgattt tcaaaataaa aatgcagtag agccagatga tgtcgatata
    26461 atcttccacg gttctcttgg aaatcctaga gtattggatt gtatagagca aggtaagaag
    26521 atgtttaagt gggatgaaaa gaaaaaactt ccaaaagaag aaggaaaata tttaagaggt
    26581 ataggtatgg caataggtgc acatggtaat ggagtttttg gtgcacatag agatgttgtt
    26641 acattgactc ttaaattaaa tgaagatggt acgttgacac tactaacagg agctcatgat
    26701 atgggtaatg gtgttgtaac tatgcaaaca atgatggtag ctgaagtttt aggaataaca
    26761 cctgataaag ttgatacatt tgaaactgat acggatgcat gtacattcaa ccttggtgac
    26821 tatgcaagta gaggggtatt tgtagaaggt ggaggagcta aaaagacagc agagaaattg
    26881 aaaaatctta tattagaaga agcagaaaaa ttacttgaaa catcaaaaga agatttatat
    26941 ctagacaatg gatgtgtaat tagtaaactt gatgaaaatg taaaagcatc actttctgat
    27001 gtcgcagtat atagtcaaag taaatcatta agagagttaa ctgttgttga agattattct
    27061 tcaccagctg gacgtacttc ttatggtgta cattttgctg aagttttagt agataaagaa
    27121 actaaggata taaaagtagt tgattttgta gctgtacatg atgttggtag agttataaat
    27181 cctctaaatt tagaaggaca attagaaggt gggattcata tgggtctagg atacgcattg
    27241 agtgaagaat tggcatttga tgaacaaggt agacctaaag ctactgattt taaaacatat
    27301 aaattattta gaactgtaga tatgccaaaa tgtcaagttg catttgtaga tggttatgaa
    27361 aaagctgggc cttttggtgg taagagtatt ggagaatgtg ccgttgtacc agttgcgcca
    27421 gctgttgcaa atgcagtata taatgcaact gatgtagaga tacattcatt accaataaaa
    27481 ttaaaatagt agaaattaaa gcagaaataa caatttattg tatttgtaaa tagataataa
    27541 aaatagattt atttggtgag gagatattat tatggcaaag atattaggta tttgtggaag
    27601 tccacgtaaa ggagcaacag aatatgcagt gttagaagct ttgaaagaag cagaaactat
    27661 acctggtata gaaactgagt attggtcagt tagagggaaa aaaataagtc cttgtgtaca
    27721 ttgtgatgca tgtataagaa aagaaactat gtgtattata aaagatgata tccaagaatt
    27781 agagcaaaaa atattggagg cagatggaat tatagtaggt tctccagttt atgatatgaa
    27841 tataactgct caacttacag cagtatttaa tagacttcgt ccaatgtatt tagttcatcc
    27901 aggaaaactt caaaataagg ttggtgcagc tataactact ggtggaacta gatatggtgg
    27961 ccaagaattg acaaaacttc ctataataaa tttcttccta atgcatgaaa tgttagtgtc
    28021 tggaggttta ggtggatgct atataggtgg tacaatatgg agtaaagatg gaaaagcaaa
    28081 aggtgcccaa gaggatgaga caggtatgga cacagtaaaa agacttggaa aaggtgcagc
    28141 agaggcagtt atggtttcta agtttggtct tgaaaaatgg aatgtcgtaa aagaagaatt
    28201 agatgttaaa aaagatgaaa aatcacctct tagagaccac taaagattta ttatattatt
    28261 gaatatatac aaatagtgat gtaatagatt attgcatcac tattttatgt tttaatttag
    28321 attttataaa ttttatatta aaaaatatag aaacaaagtg ttttgagatt tatttttaaa
    28381 ttttaaaaat gagaattaaa aagtgagaag atagaaaagg ttttttagtt attaaattct
    28441 acatacattg tatgaactaa agatagaaaa gaaaaaataa taaaagcatt gcaatttcta
    28501 tactaaagag tcatggaaaa atatctagta tatatttagt attggcatgt taattgctta
    28561 tatattaaag tataaaaatt taacaataat ttggaggtat tttagatgtt aaaagaaatt
    28621 aaatggaaag taaataattt gccaaagggc gataaggaaa attgtatcaa atttttaaat
    28681 gaagaagaaa taactaaggt tagaaatttt cacaaaagct ttcctcaata taaagaaact
    28741 ccattagcaa acctagaagg tttagcaaaa aaattaggtg ttgcaggagt atatgtaaaa
    28801 gatgaatctt atagatttgg attaaatgca tttaaagtat taggtggttc ttattctatg
    28861 ggtagatact tagctcaaag attagataca gatatatctg agttagggta tgacaaattg
    28921 acttcgaaag aaataaaaga aaaattagga gaaataactt tctttacagc tacagatgga
    28981 aatcacggta gaggagttgc ttggacagct aataaattag gtcaaaaatc tgttgtatta
    29041 atgccaaaag gttcttctga atttagatta aataaaatta aaggagaagg tgcagatgct
    29101 agtataacag acttaaatta tgatgatgca gtaagattag ctaatgatta tgcagaagct
    29161 gatgaccatg gagtaatggt tcaagataca gcatgggacg gatatgaaga aataccagca
    29221 tggataatgc aaggatatgg aactatggct caagaagcta tagagcaatt aaaagaatat
    29281 ggagtagata gaccaacaca cgtatttgtt caagcaggag ttggttcact tgctggtgca
    29341 gttcaagggt atgttgcttc aatatatgat gaatgcccaa taacagtagt agtagaggct
    29401 gatgaagctg attgttacta taaatcagca gaagctggag atggaaaacc aagatttgtt
    29461 ggaggagata tgccaactat aatggcaggg cttgcttgtg gagagccaaa tactatagga
    29521 tttgaagtat taaaaaatca tgcagcagca tttgtttcag caccagactg ggtttctgct
    29581 aaaggtatga gaacattagg taatccttta aatggtgatg aaaaggtaat ttctggagaa
    29641 tctggagcag ttacaactgg tcttttagta gcagctatgg aaagagaaga tttagcagat
    29701 ttaagaaaag atttaaaatt agatgaaaat tcaagaatac ttttaataag cacagaagga
    29761 gatactgacc ctgacaaata tagaagtata gtttgggatg gagaatatcc aagtatataa
    29821 ggaattacta aaatgcaatt attagatatt ggtttacata tttagaagat atataagttc
    29881 taaaatttag aaaatatata agtatataaa atacatacat tataaaaaat ctagggggat
    29941 aatgaaatgt taaacgaaca aagaaaacaa gaagtaactg aaatatgcca aaaattaatt
    30001 caaaatccta gttcttctgg aaatgaagaa ggtgttgtaa aagcaataga agagtctttt
    30061 gaaaaattag gttttgatag ttggtcaaga gatagatatg gaaatatagt aggatgtata
    30121 aaaggtaaca agccaggtaa gaaaatatta tttgatggac atatagatac agttccagtt
    30181 ccagatgcaa gtaaatggag tgttcctcct tttgaaggaa aaatagttga tggaaagata
    30241 tatggtagag gaacttctga catgaaaggt caatatactg caatgatgtc tgctgtagcg
    30301 tattttgcta aagacactaa tagagatttt gctggagaac tatatgtagc aggtgttgtt
    30361 catgaagaaa tatttgaagg tgtttcggct agagagataa gtaaggctgt tcaacctgac
    30421 tatgttgtaa taggtgagtc ttcagagtta aacttaaaga ttggtcagag aggtagagga
    30481 gaaatagtag tagaaacttt tggtaaacca gctcactctg ctaatccaga aaaaggt
//
LOCUS       DAMJVK010000043        29523 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.043, whole genome shotgun sequence.
ACCESSION   DAMJVK010000043 DAMJVK010000000
VERSION     DAMJVK010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29523
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.043"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(53..2719)
                     /locus_tag="PC414_002017"
     CDS             complement(53..2719)
                     /locus_tag="PC414_002017"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDF2464412.1"
                     /translation="MENTNLSKTYNPKDFEARLYKKWMDEGYFKSKPNPDKKPFTIMM
                     PPPNITGQLHMGHALDHTLQDILIRWKRMDGYEAFWLPGTDHASIATEVKVVERIKKQ
                     EGKTKYEIGREEFLKRAWEWKDEFGGKISNQLKQLGDSCDWDKERFTMDEGCNEAVIE
                     FFVSLYEKGHIYRGNRIINWCPDCKTTLSDAEVEHEEHDGNFYHIKYPLKDSEDFLEI
                     ATTRPETMIGDTGIAVNPEDDRYKHLIGKTAILPLVGRELPIVADSYVDLEFGTGAVK
                     MTPAHDPNDFEVGLRHNLEQLNTMNEDGTMNEVCGKYEGMDRFECRKAIVADLKEQGY
                     LIKIKEHNHNVGTCYRCHTVVEPRLSEQWFVKMEELAKPAIDILKKGELEFVPDKFDK
                     TYLQWLENIRDWCISRQLWWGHQIPAYYCQECGEIVVARKMPDKCPKCGSTHFKQDED
                     ALDTWFSSALWPFSTLGWPNKTEALDYYYPTSVLVTGYDIIFFWVVRMAFAGMFCMNE
                     KPFDHVLVHGLVRDSQGRKMSKSLGNGIDPLEIIEQYGADALRFTLTTGNSPGNDMRF
                     YMERVEFARNFANKLWNASRFVFMNIDEDIIKNMTRESVKEDLTLADKWIISRANNIV
                     KEATNNMDKFDLGIALQKIYDFTWSEYCDWYIEMVKPRLYGEDANAKSAALYTLTYVL
                     EKILKLLHPYMPFITEEIYTHLPTVEGCIIVSEWPKYNEEDNMAEEEDMMNLLMEGIR
                     SIRNVRAEMNVPPSKKAKLIIIPSEEKIEAIELGKDYFITLASASNVEIAKDKSNVPE
                     DAVGVVIDGVEIFIPLNELVDFEKEIERLSKEKKKLEGEIKRVNGKLANQGFLAKAPE
                     SLIEEEKAKKEKFEEMIKSVEERLTNLESKIK"
     misc_binding    complement(2795..3057)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(3353..4180)
                     /locus_tag="PC414_002018"
     CDS             complement(3353..4180)
                     /locus_tag="PC414_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2464413.1"
                     /translation="MKIKDNKAVFHVFIRIFLIAVLILTGTLLEEKINNVPVENISSK
                     GENTLELLSQSNTLGEIIYKASQEETKGPGATSEKVAYITIDDGPSKYTNALLDILKE
                     NDVKATFFMLNQNMKNHKEEVRRVLEEENSIGFHSVSHDIHKLYKSPEVTVGEFNTCN
                     NTLQEITGQSSKLIRLPYGSKPYMPEGSYNKLMANDYLIWDWNLDTQDWKSSTSQIVS
                     NILYYGRKRDELVVLIHEKEQSLNALNNIIRILKERGYTILPITENITPKNFWQENL"
     gene            complement(4228..5664)
                     /locus_tag="PC414_002019"
     CDS             complement(4228..5664)
                     /locus_tag="PC414_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase"
                     /protein_id="HDF2464414.1"
                     /translation="MNTRNDNSKILEIYKMKLFGELVKAVWNDDIEDIENLPNKILNE
                     NLKDNLNNYFSGKFDKDTMVNLIRVTMGLDVTEDTDKNLKESLHEAVNLDEVKRPIIS
                     VISDACKYCDENNKEDESCKIRSKHINCNEEDVCSACGDCVSRCKLGAISDKIEFIPM
                     IKLLKDIKSPVYAIVAPAFVGQFGKDVSPGKLRTALKKIGFEDMIEVALAADMLTAKE
                     AYDYYEHMKKDEEGCFITSCCCPIWVSLIQNSFPQILENVSPSVSPMIACGRAVKILN
                     PDAKVVFIGPCMAKKKEAMLEDIKDAVDFVLTFKELQEIFDALELNPAIMDESNRVEA
                     SLSGRVYARTGGVSKAVKLSVRRIDKNIKFEEKAFEGTKECMDGLKKVLNKEIEATFI
                     EGMGCVGGCVGGPKRILSVEEGTKNVDEYSESTNMETPFDNLNVLQFLTSLGIKRVES
                     LGEKEEEKVKKIFSRDIKSNKKENKYKK"
     gene            complement(5751..6620)
                     /locus_tag="PC414_002020"
     CDS             complement(5751..6620)
                     /locus_tag="PC414_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDF2464415.1"
                     /translation="MKLQKMGMRTIKTGISVTLCMILGDYLVENMFYSLVACIISVQD
                     TVKGSLKTGINRVEGTILGGIIGFIFAIIKAGDPFLCGLGVMCSIYLCNLFKIKSVVV
                     ACVTFLAIHLGVGNSDPVYYSLHRVLDTSVGVLFGVLVNYYVARPNYLNSVIDEFKKI
                     EELSMLLIEDKILRKENLNVEKFEEEVEKLEEIYSKLIDELDYSLSETNIEKIENALR
                     ICREIHFHMQSIELLENKLYLKEHTYKYLKKTYNVDKLDWNIDEEKSPVFNYHLMKIM
                     IEIELLRKLNKSY"
     gene            complement(6778..7431)
                     /gene="phoU"
                     /locus_tag="PC414_002021"
     CDS             complement(6778..7431)
                     /gene="phoU"
                     /locus_tag="PC414_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HDF2464416.1"
                     /translation="MVNNSLDISIDTLKEQTVLMMEKCEEAIEKSVTCMIEKDLEGAR
                     KVVEDDEEINKLREEIRDKSIELIVLKQPMARDLRFIYSLGNISIELERIGDYAANIA
                     REMLKIGGEAYIKELIDIPKMAKECKSMMSQTREALILFDASIAYDVGTKDDIVDGLY
                     EQVQIDCLKIMHENSNTIDQGVKLLLIGRYLERIGDHITNVCEKIIFAIEGKMVEIG"
     gene            complement(7459..8223)
                     /gene="pstB"
                     /locus_tag="PC414_002022"
     CDS             complement(7459..8223)
                     /gene="pstB"
                     /locus_tag="PC414_002022"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HDF2464417.1"
                     /translation="MELIDKIKMSVKDLDLFYGDKQALKKINMDIKENKVTALIGPSG
                     CGKSTFIRTLNRMNDLIEDVTIKGNISVDGEDIYTSDDVINLRTKVGMVFQKPNPFPM
                     SIYDNVAYGPRTHGLRDKKQLDKIVEESLKGAAIWDEVKDRLKSSALGLSGGQQQRIC
                     IARAIAMRPEVILMDEPTSALDPISTLKVEELIEDLKKDYTIVIVTHNMQQAARISDE
                     TAFFLNGEVIEFSDTKTMFTTPVDKRTEDYITGRFG"
     gene            complement(8242..9066)
                     /gene="pstA"
                     /locus_tag="PC414_002023"
     CDS             complement(8242..9066)
                     /gene="pstA"
                     /locus_tag="PC414_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDF2464418.1"
                     /translation="MRKFKENLLKSLVYLSALFTIVSLVVIVGFIFIKGIGNMNFNFL
                     FSNYSASGDGGILPMIITTLYTVVVSIVVATPIGILAAIYLQEYAKKGKAVTAIRFAT
                     ESLSGIPSIVYGLFGGIFFVVTLKMGYSIVAGSLTVAIIILPVIIRTTEEALKTVPQA
                     YRESSLALGATKFQTLYKVVLPSAIPGILSGVILSVGRIIGESAAILLTAGTVAKMPG
                     GIFDSARTLTVHSYLLTKESGDIATAASIGIVLIVIVLALNMLARFVAKKLNKANY"
     gene            complement(9068..9985)
                     /gene="pstC"
                     /locus_tag="PC414_002024"
     CDS             complement(9068..9985)
                     /gene="pstC"
                     /locus_tag="PC414_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HDF2464419.1"
                     /translation="MIVKSRLVGLEKNDNNGNKTKYVIEKVAKNIFLLSALVAVASLL
                     LIIGFVFYKGLRPFIFEGYSFIDFLTGADWVPSANKFGISSMIVASIFATLGALVIGV
                     PVGILTSVFIAEVAPKRLAKIMSPAVELLAGIPSVLYGVFGLAVIVPTIQSIFNLPKG
                     QSLLAIVIVLAVMMLPTIISVSETAIRAVPKAYKEGSLALGASKIETIFKVVLPAAKS
                     GVLAAVVLGVGRALGETMAVILVAGNSPVMPTAITDSVRPLTTNIALEMGYAFGTHQE
                     MLFATGVVLFTFILILNLVLNKLSNKAVN"
     gene            complement(10366..10812)
                     /locus_tag="PC414_002025"
     CDS             complement(10366..10812)
                     /locus_tag="PC414_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464420.1"
                     /translation="MAISDLAFGTECLKTSFQMLFSVISLVNINLLQAQILIAIGGLI
                     SILAMVSLTLFLSAKCKDSLTSMLIAFSICLIPMGIGVSWIGYLLPPGGIGFKISLLY
                     QMTSFNFLHLGGMSFWTPHIILFFSVIEIPIFIFLAIHTYCKHQVS"
     gene            complement(11330..12010)
                     /locus_tag="PC414_002026"
     CDS             complement(11330..12010)
                     /locus_tag="PC414_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2464421.1"
                     /translation="MQTKILIIDGDKDNCKKLKGFLEEKGISIDLAYNCEEAIGKIFS
                     NKYDLIFLEIILSDGDGWTLCKKIRNVTTCPIVYMTYINEDQSILNALNSGGDDYLIK
                     PLNLEILYAKVKAILRRMNSYVNNNENNTKLEEYNRTSGVIKLEDKIIKLTPTENKLL
                     NFFIENPEKTLTTKEIYEHVWMNEYLEDNYSVVVAVNGLRKKIEKDYKNPQKIITIRG
                     AGYYFNKE"
     gene            complement(12060..14075)
                     /locus_tag="PC414_002027"
     CDS             complement(12060..14075)
                     /locus_tag="PC414_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDF2464422.1"
                     /translation="MKKYNILKGTLIVLIVSLLAITGLNLLYKYDNKYTYKSIRASNG
                     ILTIDNDTFTKGKLVFLIDDWEFYNHKIFKPNDFTEQDIKPEYVFIGQYPDFSMRKGT
                     QSPYGNATYRMRIKNEGKDKLLSLELPEIFSASEVWINGEMVSKLGDVGTEKYRPKIK
                     NSVVSFIAKKNTEIIINVSNFSHYYSGLYYPPALGETKDISNMMFYRLLFYSIICFTT
                     FAIAIFSLSVWFLSNKSKIYLYFGCMCVFFAIHVSYPFVHLLGLPLVSFTYAVEDLSY
                     FMVVLCIICINGSISKIEENKVYRFLILPLGITMSIISIVIPIILLPYDMYFVNIYGK
                     LIDIYKYIVFIYILMSSLVSIYKKTCSEHISSYILVSVNTVFGISILFDAVTSNRFEP
                     IFTGWQTEYCGFVIVILFSVMMINRNRIIIKENEKLTRNLEHEVEVRTNELTTLLNER
                     KRFLADVAHDLKAPVSAIQAFIDLIKVGNIHVDEETRTYLYAINQKSNEVQNRVRSLQ
                     EFTSQDKSIGISEKICLNNFIKEIYENNLPDTQACGVNFETYVLEKKIYAKINKEMLT
                     RAFENLIYNALTFTPFDGKIILSMDKEDGFAIIKISDNGKGIPPENIPKVFDRFFTER
                     DNNEPKGQGLGLYIVKSIIREHGGEIFVDSELGKGTVFTIKLSLYFL"
     gene            complement(14147..14824)
                     /locus_tag="PC414_002028"
     CDS             complement(14147..14824)
                     /locus_tag="PC414_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2464423.1"
                     /translation="MYNLLVVDDDVDILNINQIYFSNIGFNVYTSETAKDAMDIVESI
                     VLDCIILDISLPDIDGYKMCNDIKEKVNIPIIFLSNYEHEEEKVRGFLAGGDDYITKP
                     YSLKELELRIYARMRQYKNITSVPNVLKFSALVINVNSRKVTFDGESIDLTTMEFEIL
                     LFLAENKEQVFSKIEIYNQVWKMPDIGDQHTVQVHIAQMRKKINSLSREHQYIQTVWG
                     KGYKFVP"
     regulatory      complement(15404..15487)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(15720..16562)
                     /locus_tag="PC414_002029"
     CDS             complement(15720..16562)
                     /locus_tag="PC414_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2464424.1"
                     /translation="MFKKKIGVLLASTMLVGALAVGCSSNSGGSGDSGDSSKVSVSGS
                     TSIGPLMETIGEKFQEKNQGVSVEVQQIGSSGGIKNAIEGTSEIGMSSRDLKDEEKSA
                     GLKETKIAIDGIALITNTANSVKNLTMDQVKGIYTGKITNWKEVGGNDAPIVIVSREE
                     GSGTRDGFQEIVGFDSEELTKDAQISDGSGNIKTTVEGNENAIGYISFGYVDEKVNSL
                     KINDVEATDANVIANKYPISRPFILVNKEDGLSEKGKALIDYILSDEGQALVSEKGFI
                     KVNQ"
     gene            complement(16814..18598)
                     /gene="pepF"
                     /locus_tag="PC414_002030"
     CDS             complement(16814..18598)
                     /gene="pepF"
                     /locus_tag="PC414_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HDF2464425.1"
                     /translation="MGTDRSTIEEKYKWKIDKMYSSKEEIEKDISKVKNLIQEVKEYK
                     GKLSESKENLYKVLNISENASRIVQNLYVYTHMKQHEDTRINDNQGRATKTEMLSTDL
                     GVATSYIVPEILAIEENKLSEYLKDEKLSFYAKYIKDILRDKPHTLSEREEEILAATS
                     ELSTIPENVYDMLAYADMEFPEIEDEEGNKVKLSHSNYSLFIKSKNSRVRKDAFEGEF
                     STYEKYKNTFASTLYGGIKSEIFYAKTRKYNSAIEASLFSDDVSLDVYNNLISAIDKS
                     IPNLNKYVELKKKFLGLNEIHMYDLYVPLTDKFDMDIPYDKAQDIILEALKPLGEEYL
                     KVIKSAFDEGWIDVYDNEGKKGGAYSWGSYDSHPYILMSYKNDLNSLFTLIHELGHSV
                     HSHYSRTSQPFLYSDYKIFVAEVASTLNELLLINYLLENSKSKDETIYLLNYYLEQFR
                     TTVHRQTMFAEFEKIVHQRVESGEPLTADEFTNIYYKLNEKYYGKSAIVDKQIGIEWA
                     RIPHFYSNFYVYKYATGFSAASALSQQILSEGSTAVDRYINFLKSGGSEYPLEQLKSA
                     GVDMTKKESIEEALNVFAELVNKLEKEL"
     gene            complement(18664..19329)
                     /locus_tag="PC414_002031"
     CDS             complement(18664..19329)
                     /locus_tag="PC414_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDF2464426.1"
                     /translation="MIDIGSKEIAIRLLLALIIGGIIGLEREKIRQFAGFRTHILIAI
                     GSCIVSITSIQLFFDYNLYTNADPARMPAQVLSGIGFLGAGAILKNKGGVKGLTTAAG
                     MWATACIGIAVGYGYYELAILGGLFVMIALFVLRIINTFLFSQEKNVLILKLSNLDSI
                     PFLYDKFEKNQLIVRNMEIECRNEEYWKVNFFVSYAKRIHLHDVMKEINMIEGVINVD
                     YLD"
     gene            complement(19480..19641)
                     /locus_tag="PC414_002032"
     CDS             complement(19480..19641)
                     /locus_tag="PC414_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl-phosphate phosphatase Spo0E family
                     protein"
                     /protein_id="HDF2464427.1"
                     /translation="MANNELEALKNEIEAVREEINTYIEYPEIFQEELVESSKKIDIL
                     INKYMYLSK"
     gene            complement(19719..20228)
                     /locus_tag="PC414_002033"
     CDS             complement(19719..20228)
                     /locus_tag="PC414_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HDF2464428.1"
                     /translation="MKTRKMTFLGLMIGYSLALYVLETYIPNPLIVIFPGAKLGLSNI
                     ITLISLMLLGVKDTVIIVTIRVILSSIFAGPLSYLLFSIGGAYLSLLFMYLASKIKDL
                     SLIGISIIGAIGHNIGQLIVASLIIENILAIGYLPFMLMASLVTGMFVGLVSRYTIPK
                     LKHFYSKFY"
     gene            20434..20652
                     /locus_tag="PC414_002034"
     CDS             20434..20652
                     /locus_tag="PC414_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDF2464429.1"
                     /translation="MTVYDLKIGEKGFINSIDGDIKLSKRLLALGCINSTEIEVKKVA
                     PLGDPIIIRFRGFDLAIRKSDAKNISLQ"
     gene            20670..22616
                     /gene="feoB"
                     /locus_tag="PC414_002035"
     CDS             20670..22616
                     /gene="feoB"
                     /locus_tag="PC414_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HDF2464430.1"
                     /translation="MINVALLGNPNVGKTTVFNLLTGSNQYVGNWPGVTIEKKEGFLG
                     KEIKVVDLPGIYAMDTFSNEEKVSKSYLENEDVDVIVNVVDASNLSRNLYLTTQLMQF
                     NKPIVILLNMLDIAESKGVNIDAKKLSEELGVIVVPIIAKKKNGVDRIEEEIKGATQK
                     AFIYQKNFGSETETYKKLESILSRCTKTTYTQKKSISDKIDNIVLNPILAYPIFIGVL
                     FLLFKFTFDWVGGPLQEGFASLIEAYISAPVSDMLSNSSPWFKSLIVDGIIGGVGGTL
                     PFFPLIFTLFFGISLFEDSGYMSRTAFLMDKVMRKVGLSGKAFIPIVMGMGCSSPAIM
                     ATRTLESEKDRRVTALIAPLMTCGAKLPIYALFVAIFFPHNAALVTTSLYLVGIVMAI
                     VVALFLNKTAFKTEAEPFILELPEYRIPTIDALIKNTWNKSKGFLIRVVTVMFAMSVV
                     IWGLSSFNVFGYTEDINVSFLATLGHIISPIFKPLGFDDWRTSVAILSGLGAKEIVVN
                     SLNILYGNLTVVLPTIFNGVTAYTFLIFTALYTPCIAALATMKKEYGNKMMFTSFAYQ
                     FILAWIMAFIVKSIGGVLFMGNSIIEALIGGIIIVLALFILFNKFKSTKNGNVCSGCS
                     ICSGCPSANSCSSKEDSNKKNNAH"
     gene            complement(22795..23850)
                     /locus_tag="PC414_002036"
     CDS             complement(22795..23850)
                     /locus_tag="PC414_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDF2464431.1"
                     /translation="MTIQDYMLETPVRMREIISNADSLFNEVKRTNLKKIIITGSGTS
                     YHSGVQVQPYLQNLLDIDVVKMYPFMITEDTFKFDNENTLVVGVSQGGSSYSTYNAMK
                     LAEDKGCKIASMAGCKNALIDEISDYILTVNCGEEKSGAKTKGYYCTKLNLMLLGLQI
                     AREKGIISSEKYNEEINKILDAINRFEAVYKLSKQWIERNKEKLVNSKEIRIIGHSDI
                     YGDTLEAALKLLETMRIPVTGYEFEEFIHGIYNAINSDSTIFILDTGKEPRVTKMIDV
                     LSGWTENVFAIGRDVTENDKNLKIDITDNPYYQTFNFIVPIQLICGEIPTLRGVDPSV
                     PKDTRFHMKLGSKKLNK"
     gene            complement(23898..24698)
                     /locus_tag="PC414_002037"
     CDS             complement(23898..24698)
                     /locus_tag="PC414_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDF2464432.1"
                     /translation="MNKLTKKELRSMFWRSFALQGAFNYERMQNLGYCYSMLPAIKKL
                     YNKKENQAKAIERHLEIFNTTTVVVPAILGITAAMEEENANNPEFDESSISAVKTALM
                     GPLAGIGDSLFWGTFRIIAAGVGVSLAKEGNIFGPLLFLLLYNIPAFALRIFGLKYGY
                     QVGVNSLERIQREGLMEKIMSMATTVGLFVVGGMVATMLSITTPLKFNLNGAEVILQD
                     ILDKIIPNMLPLTFAFVIYYMLKRKVSVTKLTIGTIVTGIALHAIGLL"
     gene            complement(24691..25482)
                     /locus_tag="PC414_002038"
     CDS             complement(24691..25482)
                     /locus_tag="PC414_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2464433.1"
                     /translation="MLVQATMLAIIAGVGILDGRIFGQSMLDRPIVTGMLVGLVLGDI
                     KSGIMIGAQLELIWMGIAGIGAATPPDVVTGGVLGTAFAILSGNGAEVALAVAVPVAV
                     LAQSLGVLVRIINSYFSQKAVFYAKQADFKKVTIMMWLPVILFFLSTSVPTFLAIMIG
                     ADKVTALINAIPKVILDGLGVAGTLLPAVGFALLMDMLLSKKMSVFFFIGFLLASYGG
                     LDITAIALFGVCVAFVLNFYINNKENNQNPQLAINTLTEGEIDFE"
     gene            complement(25522..25944)
                     /locus_tag="PC414_002039"
     CDS             complement(25522..25944)
                     /locus_tag="PC414_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HDF2464434.1"
                     /translation="MKKFLIATHGELSRELVDTSKLIIGSLTNIEYFCMTTDKSADDA
                     EKEIRSILSNNKDSEEFVVLTDVFGGSVANICTNLLLQGFKFELLTGVNLPMLLSILL
                     LVEEDTKLIVKSGLEEAKNGIVYVNELLENQNKIKNVM"
     gene            complement(25960..26439)
                     /locus_tag="PC414_002040"
     CDS             complement(25960..26439)
                     /locus_tag="PC414_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2464435.1"
                     /translation="MVSLMRIDDRLIHGQVAYGWATSLGINVILVANDEAKNDQMKAM
                     SLNLAKPSNVTLYIRGVEESGEIVEKFASSKKSNVLVLVKNTKDALVVAKNSGGVLKE
                     INVGGLRYEEGKKKLTDLVAVDDDDINNFKQIGDLGIDIEFRMLPRDKKKQLSDLIK"
     gene            complement(26571..29327)
                     /locus_tag="PC414_002041"
     CDS             complement(26571..29327)
                     /locus_tag="PC414_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2464436.1"
                     /translation="MRDEILSFVNECVLFNNFDELKKESGIITEDIVKKFNLSRTQSS
                     RLLNDLVKSDNLIKINSRPVIFLPKNKINDVVGPTKKSIYENLDELKNECKLLKQETV
                     FNSIIGYDNSLKEVIEQIKTAVLYPNGGLTIMLTGESGVGKTFLAKIIYKYSVSSNVL
                     EKNSPYNVLNCAQYYNNLELLSSLLFGHVKGAYTGANESRAGLIEQSDGGVLFLDEVH
                     RLNSEGQEKLFTFMDTGTFSRIGENGVIRKARVRLIFATTESLSEFLQTFLRRIPINI
                     YVPNIEERGTIEKRKIVEHFIYEESKVLGLPIEITQITLNAILKIKFKGNIGECKNVI
                     KYACGRAYAKNQRRNGNIFVTLQDLPRNLYIENVDIFQFQSGKSKSIIFSPKQFSIEM
                     SNGRNKRNIIYQSFIKCLDYFDRLEKNEITKEKFSKKSSNLMIRLMDNLVFSNNDENN
                     NVLMELIISIMQDIFRNLEVNNNIKYDGNNVLAFSSYLYLKDESYQLSDEYKALENKL
                     LIYLKKESYKEYSIISKISTLITNRLDMYLNNVDIIIMIIFIKSTIIRMKFNQVNATI
                     IAHGYATASSMANVCNRMLGKNIFTAIDMPIDATIADVSKQMSRYLEGDRIKNGLIVL
                     FDMGSLNLIYEDLKNKINSPMLFIDQISTISALEVGNLLMQGKNIEEIAEIMDEETKP
                     NIQLYYPQKNKEYAIISSCFTGIGTAIKIQHLLSESLIGIVDVKIIPYDYDSLVSMGD
                     KEPVFEMYDVMAIVGTANPNVNGVDFILLEDIISGKGEDDVFRIFSRVVENEKIKNIN
                     DSIVKNFSLIRVIDSLTILDSKKIIERIEEGINAIENIQKKKLSNDKKISLYVHLSCM
                     IERLVRQTEIEEYTDMDLLIKNHHSEVKLIKNAFSVLEKSYSVQIPISEIGYIYNIIY
                     GLPQ"
BASE COUNT         9467 a         5222 c         3218 g        11616 t
ORIGIN      
        1 tacaaaaagt atagaatata tactttttgt atgtttttac taattcttat atttatttta
       61 ttttagattc taaatttgtt aatctttcct caacagattt aatcatctct tcaaattttt
      121 ctttttttgc tttttcttct tcaattaaac tttcaggagc ttttgctaag aaaccttgat
      181 ttgcaagctt tccattaact ctctttattt cgccttctaa ctttttcttt tctttagata
      241 atctctcaat ttctttttca aaatcaacta attcattaag aggtataaat atttcaacac
      301 catcaataac tactccaact gcatcttctg gaacattaga tttatctttt gctatctcta
      361 catttgaagc agatgctaaa gttataaaat agtctttacc aagctcaata gcttctattt
      421 tttcttcact tgggattatt atcaatttag ctttcttaga aggtggtaca ttcatttctg
      481 ctctcacatt tcttatactt cttatacctt ccatcaataa gttcatcata tcttcttctt
      541 cagccatgtt atcttcttca ttatattttg gccattcaga tactattata catccttcaa
      601 cagtaggaag atgagtatat atttcctctg ttataaatgg catgtatgga tgtaataatt
      661 ttaatatttt ttcaagaaca taagttaatg tatataatgc tgcagattta gcatttgcat
      721 cttctccata taatctaggt ttaaccattt caatatacca gtcacagtat tctgaccaag
      781 taaaatcgta tatcttttga agtgctattc ctaaatcaaa cttatccatg ttattagttg
      841 cttcttttac aatattattt gctcttgata ttatccattt atcagctaga gttaagtcct
      901 ctttaacact ttctctagtc atgtttttaa ttatatcttc atcaatattc ataaatacaa
      961 atcttgatgc attccacaac ttatttgcaa agtttctagc aaattctact ctttccatat
     1021 agaatctcat atcatttcct ggggaatttc cagttgttaa tgtaaatctt agggcatcag
     1081 ctccatattg ctcgattatc tccaatgggt cgataccatt tcctaaagat ttactcattt
     1141 ttctaccttg agaatctctt actaatccat gaaccaaaac atggtcaaat ggcttttcat
     1201 tcatacagaa catacctgca aatgccattc taactaccca gaagaatatt atatcgtatc
     1261 ctgtaacaag tacacttgtt ggataataat aatctaaagc ttcagtctta tttggccatc
     1321 ctagagtaga gaaaggccat aatgcagaac tgaaccaagt atctaaggca tcttcatctt
     1381 gtttaaaatg agtgcttcca catttaggac acttatctgg catttttcta gcaactacta
     1441 tttctccaca ctcttgacaa taataagctg gtatttgatg accccaccat aattgtcttg
     1501 atatacacca atctcttata ttttctagcc attgtaaata agttttatca aatttatctg
     1561 gaacaaattc taattctcct tttttaagaa tatctatagc tggttttgca agttcttcca
     1621 tcttaacaaa ccattgctct gataatctag gctcaactac tgtatggcat ctataacaag
     1681 ttcctacatt atgattatgc tcttttatct taattaggta accttgttct tttaagtctg
     1741 ctacgatagc ttttctacac tcaaatctat ccataccttc atattttcca caaacttcat
     1801 tcatagttcc atcttcattc atagtattta actgctcaag attatgtctt aatcctactt
     1861 caaaatcatt agggtcatgt gctggagtca tcttaactgc accagttcca aactctaaat
     1921 caacataact atctgcaact attggaagtt ctcttcctac taatggaagt attgctgttt
     1981 ttcctattaa atgtttatat ctatcatctt ctggatttac tgctatacca gtatctccta
     2041 tcattgtctc aggtctagtt gtagctattt ctaaaaagtc ttcactatct tttagtgggt
     2101 actttatatg ataaaagttt ccgtcgtgtt cttcatgttc aacttctgca tctgataaag
     2161 tagttttgca atctgggcac cagtttatta ttctatttcc tctgtatatg tgaccttttt
     2221 catataggct tacaaaaaat tctataactg cttcattaca cccttcatcc atagtaaatc
     2281 tttctttatc ccaatcacaa gaatcaccta gttgtttaag ctgattagat atttttcctc
     2341 caaattcatc tttccattcc caggctctct ttaaaaattc ttctctacct atttcatact
     2401 ttgtcttgcc ttcttgtttt tttattcttt caacaacttt aacttctgtt gctatagaag
     2461 catggtcagt tcctggaagc caaaaagctt catatccatc catacgtttc catctgatta
     2521 atatatcttg aagtgtatgg tcaagagcgt gtcccatatg caattgccct gttatatttg
     2581 gtggtggcat cattattgta aatggttttt tatctggatt tggtttagat ttaaaatacc
     2641 cttcatccat ccatttttta tatagcctag cctcaaaatc ttttgggtta taagtttttg
     2701 ataaattcgt attttccatt gtcctgcctc ctgtatttta taaataaatt ttttaaatat
     2761 ctttagaaat aaaaaagagc tcttcatcct ctttaaaaag gacgaaaagc tcgcgttacc
     2821 accttaattc caaagcttaa aatacattaa catatcatta aattattggt taatgttatt
     2881 ttatcaactt tgactctcaa agaatttaac ggttctaacc gtctaaactt acttatagtt
     2941 cagtttagaa actccaaagc tacattcagt aaatcttata cttagaaaat cttacagcca
     3001 gtgaattttc ctctctttta gtagtattta cttactcctc ttcttctcag tctttaatat
     3061 tacatattat tatatacaaa tattattctc tttgtcaatt atttttattt tattttttct
     3121 gaattttctt aattatgtct attctctttt atatagttgt tatattttaa aatatgtttt
     3181 tacagttatt ctgtcttata atgtcaataa ttgcaagatt attttatttt aattttaatg
     3241 ctttctcatt aattttatat tatccttaca gtatacctct tattcgtatt ttttatatta
     3301 tatgttttat tttataaata atttacttta tcttttacta tatttaaatt tattataagt
     3361 tttcttgcca gaaattttta ggtgttatat tttctgtaat tggcaatatt gtatatcctc
     3421 tctcttttaa gattcttata atattattta aagcatttaa actttgctct ttctcatgaa
     3481 ttaatactac taattcatct ctttttcttc cataatataa tatattactt actatctgac
     3541 ttgtagaaga cttccaatcc tgtgtatcca aattccagtc ccaaattaaa taatcatttg
     3601 ccataagttt attatatgaa ccttctggca tatatggttt acttccgtat ggtaatctta
     3661 ttaattttga actctgacca gttatttctt gtagagtatt attgcatgta ttaaattccc
     3721 caactgttac ttctgggctt ttatataact tatgtatatc atgacttaca ctatgaaaac
     3781 ctattgagtt ttcttcttcc aagactctcc ttacttcttc cttgtggttt ttcatattct
     3841 gattaagcat gaaaaatgtc gctttgacat cattttcttt taaaatatca agtagtgcat
     3901 ttgtatattt tgatggacca tcatctattg ttatgtatgc tactttttca gaagtagccc
     3961 ctggaccttt tgtttcttct tgagatgctt tgtatatgat ttctcctaaa gtattacttt
     4021 gagaaagcag ttccagtgta ttttcacctt ttgaacttat attttcaact ggaacattat
     4081 taattttttc ctccaataat gttccagtta aaattaaaac agctataaga aatatcctta
     4141 taaatacatg aaaaacagcc ttattatctt taattttcat cccccatatc tcccttttat
     4201 aaaagcataa gtttaagact taaaaagtta ttttttatat ttattctctt tcttattact
     4261 ttttatatct ctactaaata tttttttaac cttttcttct tctttttctc ctaaagattc
     4321 tactcttttg attcccaaag aagttaaaaa ttgtaataca tttaaattat caaatggagt
     4381 ttccatattc gtactttcac tatattcatc aacattctta gtaccttctt ccacacttaa
     4441 aatacgcttc ggacctccta cacagcctcc tacacacccc attccttcta taaatgtagc
     4501 ttctatttct ttatttagaa ctttctttag accgtccatg cactcttttg ttccttcaaa
     4561 tgccttttct tcaaatttta tatttttgtc aattcttcta actgataact taactgcttt
     4621 acttacacct cctgttctag cataaactct acctgaaagg gacgcctcta ctctattact
     4681 ctcatccatt attgctggat ttaactctaa tgcatcaaat atttcttgca actctttaaa
     4741 tgtaagcaca aaatcaactg catcttttat atcttctaac attgcctctt tcttttttgc
     4801 catacaagga cctatgaata caactttagc atctggattt aaaattttta cagctctccc
     4861 acaagcaatc ataggagaaa cggatggtga tacattctcc aatatttgag gaaaactatt
     4921 ttgtatcaaa ctaacccaaa taggacagca acaactagtt ataaagcatc cttcttcgtc
     4981 ctttttcata tgctcataat aatcatatgc ttcttttgca gtaagcatat ctgctgccaa
     5041 agcaacttct atcatatctt caaatcctat cttttttaat gcagttctta atttaccagg
     5101 gcttacatct ttcccaaact gtcctacaaa agctggtgca actattgcat atacagggct
     5161 ctttatatct tttaacaatt ttatcattgg tataaattct attttatctg aaattgcacc
     5221 caacttacat cttgatacac aatcaccaca agcactacat acatcttcct cattacaatt
     5281 aatatgctta cttcttatct tacatgactc atcttcctta ttattttcat cacaatattt
     5341 gcaagcatct gatattacag atattattgg tcttttgact tcatctaaat ttacagcttc
     5401 atgtagtgac tcttttaaat ttttatctgt atcttctgtc acatccaatc ccatagttac
     5461 cctaattaaa tttaccatag tatctttatc aaattttcca ctaaaataat tatttaaatt
     5521 atcctttaaa ttttcattta aaattttatt aggtaagttt tcaatatcct ctatgtcatc
     5581 attccacact gcttttacaa gttcgccaaa aagcttcatt ttatatattt ctagtatttt
     5641 agaattgtca tttctagtat tcataaaaaa tagtacctcc ataaattaaa cttatatatg
     5701 tttaatattt acaaagatac tattttttat ttattattta actactatta ttaatatgat
     5761 ttatttagtt ttctcaacaa ctctatttca atcattattt tcattagatg atagttgaat
     5821 acagggcttt tttcttcatc tatattccag tctagcttat ccacattata tgttttctta
     5881 aggtatttat atgtatgctc tttcaaataa agtttatttt caagtaactc tatagactgc
     5941 atatgaaaat gtatttctct acatatcctt aaggcattct caattttttc tatattagtt
     6001 tcactcaaac tataatccaa ctcatcaata agttttgaat atatttcttc taatttttct
     6061 acttcttctt caaatttctc tacatttaaa ttttcttttc taagaatttt atcttcaata
     6121 agcaacattg aaagttcttc tatcttttta aactcatcta taacactatt tagataattt
     6181 ggtctagcaa cataataatt aacaagtact ccaaacaaaa cacccacaga tgtatctaac
     6241 accctatgta aagaataata cacagggtca ctatttccta cacctagatg tatagccaga
     6301 aaggttacac aggcaactac tactgacttt attttaaaca gattgcataa atatattgag
     6361 cacattactc ctagaccaca taaaaatggg tctccagctt taatgatggc aaatatgaat
     6421 cctattatac cacctaaaat agtaccttcc actctattga ttcccgtctt taaagaacct
     6481 tttactgtat cttgaacaga aattatacat gctaccagag aataaaacat attctctact
     6541 aaatagtcac caagaatcat acataaagtg acactaattc ctgtttttat agtcctcata
     6601 cccatctttt gaagtttcaa agttccacct cctaaaaagc accttatata aaatacgcat
     6661 cctcatattt aatatgttcc tagtatatca caatttttta aatttaccca ttaatcatat
     6721 ttttttatca attctttctt aatttagaca ataaaaaaac tgctttaaag cagtttttta
     6781 tcctatctca accattttac cctcaattgc aaaaattatc ttctcacata catttgttat
     6841 atggtctcca attctttcaa ggtatcttcc tataagtaga agttttaccc cttggtctat
     6901 ggtatttgag ttttcatgca ttatttttaa acaatcaatt tgtacttgtt catacaatcc
     6961 atctacaata tcatctttag ttccaacatc ataagctata cttgcatcaa ataagattaa
     7021 agcttctcta gtctgagaca tcatagactt acattctttt gccatcttag gaatatctat
     7081 tagctctttt atataagcct ctccacctat cttcaacatt tctctagcta tatttgctgc
     7141 ataatctcct attctttcca gctctataga tatattacct aatgaataaa taaatcttaa
     7201 gtcccttgcc attggttgct ttaatactat tagctctata ctcttatctc ttatttcttc
     7261 tcttaattta tttatttctt catcatcttc tactactttt cttgcaccct ctaaatcctt
     7321 ctctatcata caagtcacag acttttctat tgcttcttca catttttcca tcataagcac
     7381 tgtttgttct tttaaagtat caatacttat atctaagcta ttattgacca ttgtctaact
     7441 cctttatagt tatatatttt aaccaaatct acctgttata tagtcttctg ttcttttatc
     7501 tacaggagtg gtaaacatag tttttgtatc gctaaattct ataacctctc catttaagaa
     7561 aaaagctgtt tcatctgata tacgagcagc ttgttgcata ttatgtgtaa ctatgactat
     7621 tgtgtagtct ttttttaaat cctctataag ttcctctact tttaatgttg aaattgggtc
     7681 taaagcagat gtaggttcat ccataagaat aacttctggt ctcatagcaa ttgctcttgc
     7741 tatacatata cgttgttgtt gaccaccaga aagtcctagt gcagatgatt taagcctgtc
     7801 tttaacctca tcccaaatag ctgcaccttt taagctttct tctacaattt tatctaattg
     7861 cttcttatct cttagtccat gtgtacgagg accatatgcc acattatcat atatactcat
     7921 aggaaatgga tttggctttt gaaataccat tcctacttta gtacgaagat ttataacatc
     7981 atctgaggta tatatgtcct ctccatcaac agaaatgttt cctttaattg ttacatcttc
     8041 gattaagtca ttcattctat ttaacgttct tataaaagtt gattttccgc atccagatgg
     8101 acctattaaa gcagtaacct tattctcttt tatatccata tttattttct ttaatgcttg
     8161 cttatcacca taaaataaat ccaaatcttt tacactcatt tttatcttat cgattaattc
     8221 cattattcac acctcttaaa tttaataatt agctttattt aatttcttag caacaaatct
     8281 tgctagcatg tttagtgcta atacaattac tattaaaaca attcctatac tggctgctgt
     8341 tgctatatct ccactctctt tagtcagtaa gtatgagtgt acagttaatg ttcttgcact
     8401 atcaaatatt ccacctggca tcttggctac agtacctgct gtaagtaata ttgcagctga
     8461 ttctccaatt atacgaccaa ctgataagat tactccagat aaaattcctg gtattgcact
     8521 tggaagtact accttatata aagtttgaaa ttttgtagct cctaatgcca atgatgattc
     8581 tctataagct tgaggaactg tttttaaagc ttcttctgtt gtacgaatta ttactggtaa
     8641 tattattatt gcaactgtaa gtgaacctgc aactatggaa tatcccattt ttaatgtaac
     8701 tacaaagaaa attcctccaa ataatccata tactatagat ggtatccctg ataaactttc
     8761 agttgcaaat cttatagctg taactgcttt tcctttttta gcatattctt gtaaatatat
     8821 agcagccaat attcctatag gagttgctac aactatagaa actactactg tatataatgt
     8881 tgtaattatc attggaagta taccaccatc acctgatgct gaataattgc taaataagaa
     8941 attaaaattc atgttgccaa ttccctttat aaatataaat cccactataa ctactaatga
     9001 tactattgta aatagtgctg atagatatac taatgacttc aatagattct ctttgaactt
     9061 tctcatctta atttaccgcc ttatttgaaa gtttatttaa aactaagttt aaaattaata
     9121 taaatgtaaa caatactact ccagttgcaa atagcatttc ttgatgtgta ccaaatgcgt
     9181 atcccatttc aagtgctata ttagttgtaa gtggtcttac actatctgtt attgcagttg
     9241 gcataactgg tgagttgcct gcaacaagta ttactgccat tgtttcacct aaagctcttc
     9301 ctactcctaa cactactgcg gctaatactc cagattttgc agcaggtaat acaactttga
     9361 atattgtttc tatttttgat gcaccaagtg ctaatgaacc ttctttgtat gcttttggta
     9421 ctgctcttat tgctgtttct gatacagata ttattgttgg taacatcatt actgctaata
     9481 ctattactat tgctaacaaa ctttgacctt ttggaagatt aaatatactt tgtatagttg
     9541 gaactatcac tgcaagacca aatacaccat ataatactga aggtatacct gctaaaagtt
     9601 caactgctgg tgacattatt tttgctaatc ttttaggtgc tacttctgct ataaatacag
     9661 aggttaatat accaacaggt actcctataa caagtgctcc aagtgttgca aatattgatg
     9721 ctactatcat agatgatatt ccaaatttat tggcagaagg aacccaatct gcaccagtta
     9781 aaaagtctat aaatgaatat ccttcaaata taaatggtct taatccttta taaaatacaa
     9841 atcctattat caaaagtaaa cttgcaactg ctactaaagc actaagtaga aaaatatttt
     9901 ttgctacttt ttcaattacg tattttgtct tattgccatt attgtcattt ttttctaaac
     9961 ctactaatct agattttact atcataatac cctcccatta aaatatactt atcgattttc
    10021 gttctgtatt caaaatttaa ttttttataa ttcgtcttca tcaaatttcc tgtactttta
    10081 ttctactttt tttaagttaa atctatatta ggattaagtt aagcaagtgt aaaattatat
    10141 ttacttttta acattaattt tacttttaaa actatatttg acatttactt tacaatatac
    10201 tgttgtttct ctatattaaa atagcagata tagataataa tacagtttat gtataattta
    10261 ttacctatat ctgctattaa gatttgttaa ctaaatttaa cttttatact acatatataa
    10321 ttattgtatc ctctagttta tatcactatt atttccatca aattgttacg atacttgatg
    10381 tttgcaatag gtatgaatag ccaaaaatat aaaaataggt atttctatca ctgagaaaaa
    10441 cagtataatg tgtggtgtcc aaaaactcat acctcctaag tgtaagaaat taaatgatgt
    10501 catttgatat aataaactaa tcttaaaacc aattccccct ggcggtaata ggtatccaat
    10561 ccaactaaca cctattccca taggaattaa gcaaatagaa aaagcaatca gcatagaagt
    10621 aagagaatcc ttacattttg cagataaaaa taatgttaaa ctaaccattg caagtattga
    10681 aattagacca ccaatagcta taagaatctg tgcttgtagt aaatttatat ttactagact
    10741 aatgacagaa aacaacattt gaaatgaggt tttcaaacat tcagtaccaa aagctaaatc
    10801 agaaattgcc aaatagatag aaatacatat aacaaacatt attataaata tagaaaatag
    10861 agctaatatt ttagtaaacg ctagacaaat atgtccatgt ttcgtacaac gaaggatgct
    10921 atctgattgt gtctgatact caccagaaaa agtaggtgca gcaattgcaa catatataat
    10981 taataatata aaaatataaa atattatata atcgaaagca tcttctggat ttattaagaa
    11041 attcttaatc ttattattta actattttat tatttaatat atattaaagg taaaaaaata
    11101 atatatagat ttagatatac aatttatcta tcttttcaaa tataatctat aaaataatag
    11161 ttccattacg cattttttta tataataaaa ctgactgtaa tttaactggc aaagtctaga
    11221 agaataggct ttatcaatta aattacagtc agctatttta taataattta gtttagatat
    11281 tgttagtatt tcacatttat aacaattatt aatgatatta atatatttat tattctttat
    11341 taaaataata tcctgctcct cttattgtta taattttctg aggattttta taatctttct
    11401 ctattttctt tcttaatcca ttcactgcaa ctaccacact atagttatcc tctaagtatt
    11461 cattcatcca tacatgttca tatatctctt tagtagtcaa agtcttttca ggattctcta
    11521 taaaaaagtt taacaactta ttttctgttg gagtcaattt tattatttta tcttctaact
    11581 ttattactcc actagttcta ttatattcct ctaattttgt attattttca ttattattta
    11641 catatgagtt cattcttctt aaaatagctt ttacttttgc gtataatatt tctaaattta
    11701 gaggctttat taagtaatcg tctccaccag aatttaaagc atttaaaatg ctttggtctt
    11761 catttatata agtcatataa acaattggac aagtagttac atttctaatt tttttacaaa
    11821 gagtccatcc atcaccatca cttagaatta tttctaaaaa tattaaatca tatttattgc
    11881 taaatatttt tccaatagct tcttcacaat tatatgctaa atctatactt attccttttt
    11941 cctccagaaa tccttttagc ttcttgcaat tatccttgtc tccatctatt atcaatatct
    12001 tagtttgcat tataatcact cccaaatatc attattcttt tatattaaat aagctattat
    12061 tataaaaaat atagacttaa ttttattgta aaaacagttc cttttcctag ttctgaatct
    12121 acaaagattt ctccaccatg ctctcttata atggatttga caatatatag ccctaaccct
    12181 tgcccttttg gctcattatt gtctctttct gtaaagaatc tgtcaaatac ctttggtata
    12241 ttttctggag gtataccttt tccattatca gaaattttaa taattgcaaa tccatcttct
    12301 ttatccattg ataaaattat ctttccatca aatggagtaa aggtcaaagc attataaatt
    12361 aaattctcaa atgctcttgt aagcatctct ttatttatct ttgcatatat ttttttctcc
    12421 aatacgtaag tttcaaagtt tacaccacat gcttgtgtat ctggcaaatt attttcatat
    12481 atctctttaa taaaattatt cagacatatt ttttcactaa taccaatgct tttatcttgt
    12541 gatgtaaatt cttgcaaact acgtactcta ttttgtactt catttgactt ttgatttata
    12601 gcataaagat aggttcttgt ctcttcatct acatgaatat taccaacctt tatcaaatct
    12661 ataaatgctt gtatagccga aacaggtgcc tttaaatcat gtgctacatc tgccaagaat
    12721 cttttacgtt catttaacaa tgttgtaagt tcattagttc tcacttcaac ttcatgttca
    12781 agattccttg taagtttttc attctctttt attattattc tattcctgtt tatcatcata
    12841 acagaaaata aaataacaat tacaaaccca caatattctg tttgccaccc tgtaaatata
    12901 ggctcaaatc tattactcgt aactgcatca aataatatgc taattccaaa aacagtgttt
    12961 actgaaacta aaatataaga acttatatgc tcagagcatg tctttttata tattgaaacc
    13021 agcgaagaca ttaatatata tataaataca atatatttat atatgtctat taacttccca
    13081 tatatgttga caaagtacat atcatatgga agaagaataa ttggtatcac tatagatatg
    13141 atactcattg taattcctaa tggtaaaatt aaaaatctgt acactttatt ttcttctatt
    13201 ttggatatac ttccatttat acaaataata cataaaacta ccataaaata tgacaaatct
    13261 tcaaccgcat atgtaaagct taccaaaggc agtcctaaca aatgtacaaa gggataggat
    13321 acatggatgg caaagaaaac acacatacat ccaaaatata agtaaatttt acttttgttt
    13381 gaaagaaacc atacagacaa tgaaaatata gctattgcaa aagttgtgaa gcaaattata
    13441 ctataaaaaa gtaatcgata aaacatcata ttacttatgt cctttgtttc tcctaatgca
    13501 ggcggataat acaaaccact ataataatgt gaaaaattcg atacattgat tataatttct
    13561 gtatttttct ttgcaataaa acttactact gaatttttaa ttttgggtct atatttttct
    13621 gttcctacat ctcctaattt agaaaccatt tctccattta tccatacttc acttgcggaa
    13681 aaaatctcag gaagctctaa acttagtagc ttatccttgc cttcattttt tattctcata
    13741 cgataagtag cattcccata tggtgactga gtcccctttc tcatagaaaa atctggatat
    13801 tgacctataa aaacatattc tggttttata tcttgctctg taaaatcatt cggtttaaat
    13861 attttatgat tataaaactc ccagtcatct atcaaaaaga caagtttacc tttagtgaaa
    13921 gtatcattat ctatagttaa aattccatta cttgctctaa tagacttata tgtatattta
    13981 ttatcatatt tatacagaag atttaatcct gttattgcca acagagagac tatcaataca
    14041 atgagagttc cttttagtat attatacttt ttcattgtat caaccacatc tctttcatag
    14101 tagtattaaa taaaacaaga actatttatt aattacagta tctcacttat ggtacaaact
    14161 tatatccttt tccccatacg gtttgaatgt attgatgctc cctgcttaaa gaattaattt
    14221 tttttctcat ctgtgcaata tgaacctgca ctgtatgttg atctccaata tcaggcattt
    14281 tccaaacttg gttatatatc tctatcttcg aaaatacttg ttctttgttt tcagctagaa
    14341 acaataatat ttcaaattcc atagtagtta aatctatact ttctccatca aaagttactt
    14401 ttcttgaatt tacatttatt actaacgctg aaaactttaa cacattagga actgatgtaa
    14461 tatttttgta ctgcctcata cgagcatata ttctaagttc aagttctttt aaactataag
    14521 gttttgttat ataatcatcc cccccagcta aaaatccccg aactttttct tcttcatgct
    14581 cataattaga taaaaatatt ataggtatgt taaccttttc ttttatatca ttgcacattt
    14641 tatatccatc tatatctggc aatgaaatat ccaaaattat acagtctaaa actatactct
    14701 ctactatatc catagcatct ttagcagtct ctgatgtata tacattaaaa cctatattag
    14761 aaaaataaat ttgatttatg tttaaaatat caacatcatc atctacaacc agtaaattat
    14821 acattacacc actccattca aaggtatttt aattataatt ccaagtttta tagttattac
    14881 aaattataat gttttatttt catatataac tggtattatt attttataat atctttacaa
    14941 actagactat aatacttaag tatttttgaa taattcttga atattgtatt aaacattaag
    15001 attcattatt aaatactaat taaagtctcc gattgttgga gaatggagct taaagtcatt
    15061 ttcacgtata aatttattat ttttctagaa acttgtctat tggctagttt gagtattgat
    15121 ttatatgatt tatatggtta taatataaaa taaattgtat tattttggta aatatattgt
    15181 tacaaaaggt ttatattctg catctcaatg tttataaaaa ctttattttc ttattataat
    15241 actattattc ttttaaaaaa atttcccttt tccaacaaat ggctacttta aaagccaaaa
    15301 ttttacctat caataaaaat cttaacgact aatactctta ttttcaaatt ttgcaacata
    15361 tttgttaatt ttaaaacaaa aaaagactat gcaatataaa aattatattc atagccggtt
    15421 gcaccactaa ctccatatat ctctagatgc ccaataataa gatatacttc tcagtttcta
    15481 aaatattttt attaaaaatc ttttatatat tttcataaat ttctttttta ttctaacatt
    15541 gtaatattat tttgtcaata tttccatatt cttagagata agatactaaa atacctattt
    15601 tatctaaaga tatttatatt tctacattca ataatagaca ataccctgca agttaaagca
    15661 tcatttttat gagcttatct cacagggtat tatatgttta ctttatatta taactcttgt
    15721 tattgattaa ctttaataaa tcctttttct gaaacaagtg cttgtccttc atcacttaat
    15781 atataatcta ttaaagcttt acctttttct gacaaaccat cttctttatt aactaatata
    15841 aatggtcttg atattggata tttattagct attacatttg catcagttgc ttcaacatca
    15901 tttattttta aactatttac tttctcatca acatatccaa atgatatata tcctatagca
    15961 ttttcatttc cttcaactgt agtttttata tttcctgaac catcacttat ttgtgcatct
    16021 ttagttagtt cttctgaatc aaaccctaca atttcttgga atccatctct agttcctgaa
    16081 ccttcttctc ttgatactat tactattggt gcatcatttc ctcctacttc tttccagtta
    16141 gtaatctttc ctgtatatat acctttaact tggtccattg tcagattttt tactgagttt
    16201 gcagtattag ttatcaatgc tataccatct attgctattt tagtttcctt taatccagca
    16261 cttttttctt catcttttaa atctcttgaa ctcattccta tttcagatgt tccttctata
    16321 gcatttttta tacctccaga agaaccaatc tgttgtactt ctacactaac accttgattt
    16381 ttttcttgaa acttttctcc gatagtttcc ataagaggac ctattgaagt tgaacctgaa
    16441 acagaaactt tgcttgaatc tcctgaatca cctgaacctc ctgagttaga actacatcct
    16501 actgctaaag ctccaactaa cattgtacta gctaaaagaa cgcctatttt ctttttaaac
    16561 attttactta cccccgctta tattttaatt ttttgttgtc atttttctat attttaataa
    16621 tacagattct ttgttaaatg aatattagag cagggttaag taaatgtaaa tattgttaaa
    16681 tacatgtaaa aacttaattt tatatgattt attcttaatt agattaactt tttaaagtta
    16741 ctttaggcag atataaacat aatacaactc atattatcag aagtcttcat tgaagctatc
    16801 aacctatcac atcctataat tctttttcta gtttattaac taattcagca aatacattta
    16861 aggcttcctc tatagattct tttttcgtca tatctacacc agcagatttt aattgttcta
    16921 gtgggtattc tgaccctcca ctttttaaga agtttatata tctatctact gcagtacttc
    16981 cttctgaaag tatctgttga cttaaagcac ttgcagcaga aaaccctgtg gcatacttat
    17041 atacatagaa gtttgaataa aaatgaggta ttctcgccca ttctattcct atttgtttat
    17101 ctactattgc agattttccg taatactttt catttaattt gtagtatata ttagtaaatt
    17161 catcagcagt aagtggttct ccactctcta ccctttgatg tacaattttt tcaaattcag
    17221 caaacatagt ttgtctatga actgtagttc taaattgttc taaataataa tttaataagt
    17281 atatagtttc atccttagac ttagaatttt ctaataaata atttataaga agtaattcat
    17341 ttaatgtaga tgcaacttct gcaacgaata tcttatagtc cgaatataaa aatggttgac
    17401 ttgttcttga ataatgactg tgaacagagt gccctaattc atgaattaaa gtgaatagag
    17461 aattaaggtc atttttatag ctcattaata tataaggatg tgaatcataa cttccccaag
    17521 aataagctcc tcctttttta ccttcattat catatacatc aatccaccct tcatcaaaag
    17581 cactttttat gaccttcaag tattcttctc ctaaaggttt aagtgcctct aatatgatat
    17641 cctgtgcttt atcatatggt atatccatgt caaatttatc tgtcaaagga acatacaaat
    17701 catacatgtg tatttcattc aatcctaaaa atttcttttt aagctcaaca tatttattta
    17761 gattaggaat acttttatct atagctgata taagattatt gtaaacatct aaacttacat
    17821 catctgaaaa taaagacgct tctattgctg aattatactt tctagttttt gcgtaaaata
    17881 tctctgattt tattccacca taaagcgttg aggcaaatgt atttttatat ttctcatatg
    17941 ttgaaaattc accttcaaaa gcgtcttttc taactctact attcttactt tttataaata
    18001 aagagtagtt tgaatgagaa agcttaacct tattcccctc ctcatcttct atctcaggaa
    18061 attccatatc tgcatatgct aacatatcgt atacattttc tggaattgtc gataattctg
    18121 atgtagctgc aagtatttct tcctctcgtt cactcaaagt atgtggttta tctctcaata
    18181 tatcctttat atattttgca taaaaggata gcttctcatc ttttaagtat tcacttaact
    18241 tattctcttc tatcgccagt atttctggaa ctatataaga agtagccaca cctaaatcag
    18301 tagataacat ttctgtctta gtggctcttc cttgattatc attaattcta gtatcttcat
    18361 gttgtttcat atgagtatat acatataaat tttgaacaat cctagatgca ttttcagaga
    18421 tatttaatac tttatataag ttctccttac tctcagaaag cttcccttta tattctttta
    18481 cttcttggat aagatttttt actttactta tgtctttttc aatttcctct ttactggagt
    18541 acatcttatc tatcttccat ttgtactttt cttctattgt acttctatca gttcccatat
    18601 catacacctc tattttaatt tgttttatat tttattcact ttacaaaata tattgtatat
    18661 attttagtcc aaataatcta catttattac tccttctatc atatttattt ctttcataac
    18721 gtcgtgtaag tgtattctct tagcataaga tacaaaaaaa ttaaccttcc agtattcttc
    18781 attacgacat tctatttcca tatttcttac gataagttga tttttttcaa atttatcata
    18841 taaaaaaggt atagaatcca aattgcttaa ttttaatatc aatacattct tttcttggct
    18901 aaataaaaac gtattgatta tcctaagcac aaataatgct atcattacaa ataatccacc
    18961 tagaattgcc aattcataat aaccatatcc aactgctata ccaatacatg cagttgccca
    19021 catcccagca gctgtagtaa gcccttttac tccaccttta ttttttaaaa ttgctcctgc
    19081 acctaaaaaa ccaattccag ataatacttg agcaggcatt cttgctgggt cagcatttgt
    19141 atacaaatta taatcaaaga ataattgaat cgaagttatt gaaacaatac aagaacctat
    19201 tgcaattagt atatgtgtcc taaatcctgc aaactgtctt attttttctc tctctaagcc
    19261 tattattcct cctattatta gtgctaatag taaccttatt gctatctcct tacttcctat
    19321 atcaatcata atataaaacc ctcctgactt aattacaact tatgtcctac agataattta
    19381 cacatcagaa atactatttt atatcttaat attattatta atatatgtaa aatatagcca
    19441 tctgttaatt tttgatacaa aaaaacccac aactgtgggc tatttgctta ggtacatgta
    19501 tttatttatt aatatatcta ttttcttact tgattctact aactcttctt gaaatatttc
    19561 tggatattct atgtatgtat ttatctcttc cctaactgct tcaatctcat tttttaacgc
    19621 ttctaattca ttgttcgcca taataatcac cactattaca aatttgtata tttttatata
    19681 cattttatca aacaatatta tattttgtca atattatgtc aataaaactt actataaaaa
    19741 tgctttaatt taggtattgt atacctggat actaagccta caaacatacc agttactaat
    19801 gatgccatta acataaatgg taagtaccct atagcaagta tattttctat gattaaactt
    19861 gcaactatta attgacctat attatgccct attgcaccta taatgcttat ccctattagt
    19921 gataaatctt ttattttact cgctaaatac ataaataaaa gactcagata agctcctcct
    19981 atactaaata gtagatatga taaagggcct gcaaatattg atgacaaaat taccctgata
    20041 gtaacaatta taacagtatc tttgacacct aacaacatta atgaaattaa agttataata
    20101 ttgctcaaac ccaattttgc ccctggaaat ataactataa gaggatttgg tatatatgtc
    20161 tctaatacat ataaagctaa actataccct atcataagac ctaaaaatgt catttttcta
    20221 gttttcatta agttaactcc tccaaatttt atctatcatc ttacattata atacaaaata
    20281 tataaaaaca cctaatttag actaaaatat ttttaatcta tttagaataa atattagtta
    20341 tttcaatgtg caattacctc tataaatctt gactttttgt atcatacttg ttacaatttt
    20401 aatgataatg aatttcatta ggaggtgtta ataatgactg tttacgattt aaaaatagga
    20461 gaaaaaggat ttattaatag tatagatgga gatattaaat tatctaaaag attgcttgca
    20521 cttggatgta taaatagtac tgaaatagaa gttaaaaagg ttgccccact tggagaccca
    20581 attataatta gatttagagg ctttgacctt gcgattagaa aatctgatgc taaaaatatt
    20641 tctttacaat aatagaggag gagctacccg tgattaatgt agcattatta ggaaacccaa
    20701 atgtcggtaa aactactgtt tttaatctac ttacaggttc aaatcaatat gtaggtaact
    20761 ggcctggtgt tactatagaa aaaaaagaag gatttcttgg aaaagagata aaggttgtcg
    20821 atttacctgg tatatatgca atggatactt tctcaaatga agaaaaggtt tctaaatcat
    20881 atttagaaaa tgaagatgtt gatgtaatag taaatgtagt tgatgcttct aatttatcaa
    20941 gaaaccttta cttaacaact caacttatgc aatttaacaa gcctatagtt atattgctta
    21001 atatgttaga cattgcagaa tctaaaggag ttaatataga tgccaaaaaa ttaagtgaag
    21061 aacttggagt aattgttgtt ccaattatag caaaaaagaa aaatggtgtt gataggatag
    21121 aagaagagat taagggagct acgcaaaaag cttttatata tcagaaaaac tttggaagtg
    21181 aaacagaaac atataaaaaa ttagaatcca ttttatcgag atgtacaaaa actacatata
    21241 cacaaaagaa atctatcagt gataagatag acaatatagt cttgaatcct atattagcat
    21301 atccgatatt tattggtgta ttgttcctct tatttaaatt tacctttgac tgggtcggag
    21361 gaccactaca agagggattt gctagtctta tagaggctta tatttctgca cctgttagtg
    21421 atatgttatc taactctagc ccatggttta aatcacttat agttgatgga ataattggag
    21481 gtgttggtgg tactttacca ttcttccctt taatatttac attattcttt ggtatatcgt
    21541 tgtttgaaga tagtggatat atgtcaagaa cagcattttt aatggataaa gttatgagaa
    21601 aggttggtct atctggtaaa gcttttatac ctatagttat gggtatggga tgttcttctc
    21661 cagctataat ggcaactaga acattggaaa gcgaaaaaga tagaagagtt acagctctta
    21721 ttgcaccact tatgacttgt ggagcaaagc tacctatata tgcactattt gtagctatat
    21781 tctttccaca taatgctgct ttagtaacta catcactgta tttggtgggt atagttatgg
    21841 caattgtagt tgcattattc ttaaataaaa cagcctttaa aacagaggct gaaccattta
    21901 tattagaatt accagaatat agaatcccta ctatagatgc acttattaaa aatacttgga
    21961 ataaatcaaa aggtttcttg ataagagttg ttactgtcat gtttgcaatg tcagtcgtaa
    22021 tttggggact ttcttcattc aatgtttttg gatatacaga agatataaat gtaagcttct
    22081 tggctactct aggtcatatt atatctccta tattcaaacc tcttggtttt gatgattgga
    22141 gaacttcagt tgctatttta agtggtcttg gagcaaaaga aatcgtagta aactctttaa
    22201 atatactata tggtaatcta actgttgtct taccaacaat atttaatgga gttactgcat
    22261 atacattctt gatatttact gctttatata ctccatgtat agctgcactt gcaactatga
    22321 aaaaagaata tggcaataaa atgatgttta catcatttgc atatcaattt atactagcat
    22381 ggataatggc ttttatagtt aaaagtatag gtggagtact atttatgggt aattcaataa
    22441 tagaagcttt gataggtgga ataattatag tccttgcact ctttattctg tttaataaat
    22501 tcaagtctac aaaaaacgga aatgtctgta gtggatgttc tatttgttct ggatgtccaa
    22561 gtgctaattc ttgctcaagt aaggaagaca gcaataaaaa aaataatgca cactaatata
    22621 taaatatttt agtttaaaat aagataaaag tgggtgtctc aaaattaact ttttagttca
    22681 tgaggcactc ttttttatag aaaatcaaat atattttcat catttcaaca gtaaaaaaga
    22741 gcttatctag taaaaaagag gcttatctct attttgagac aacctctttt tttgttactt
    22801 attcagtttc ttgctaccta acttcatgtg gaacctagta tcttttggaa cacttgggtc
    22861 tacacctctt aatgtaggta tttccccaca tattagttgt ataggtacta taaaattaaa
    22921 tgtttgatag taaggattat ctgtgatatc aatctttaaa tttttatcat tttctgtaac
    22981 atctctacct atagcaaata cattttccgt ccaaccagaa agtacatcaa tcatcttagt
    23041 tactctaggt tcttttcctg tatctagtat aaatatagta gaatcactat ttatagcatt
    23101 atatatacca tgtataaact cttcaaactc atacccagtt acaggtattc tcattgtttc
    23161 caataatttt aatgctgctt ctaatgtatc tccatatata tcagagtgac ctattattct
    23221 tatttcttta gaattaacta acttttcttt gtttctttca atccattgtt tagagagctt
    23281 atacactgct tcaaatctat taattgcgtc taatatctta tttatttcct cattgtactt
    23341 ttcactagat attatacctt tttctctagc tatttgaaga cctaataaca ttaagtttaa
    23401 ctttgtacaa taatatcctt ttgttttagc tccagatttt tcttcaccac aatttactgt
    23461 taatatataa tcagaaatct catctattaa agcattctta caacctgcca tagatgctat
    23521 tttacagccc ttatcttcag ctaacttcat agcgttgtat gtagaataac tactcccacc
    23581 ttgagataca cctactacta gggtattttc attatcaaac ttgaatgtat cttcagttat
    23641 cataaatgga tacattttca caacatctat atctaataaa ttttgtaaat atggttgtac
    23701 ttgcactcct gagtgatagc tagtacctga acctgttatt ataatctttt ttagatttgt
    23761 tctcttaact tcattaaata agctatctgc attagatata atttctctca ttctaactgg
    23821 tgtttctaac atataatctt gtattgtcat tttatacacc ttccttatct atatattcaa
    23881 taaattataa ttaatttcta taacaaacct atagcatgta aagcgattcc tgttactata
    23941 gtgcctatag ttaattttgt tacacttacc tttctcttta acatgtagta tattacaaat
    24001 gcaaaggtta aaggtaacat attaggtatt attttatcta gtatatcttg taaaattact
    24061 tctgcaccat ttaagttgaa tttcagagga gtagttatac tcaacatagt tgcaaccatt
    24121 cctcctacaa cgaataaacc aacagttgta gccatagaca ttattttctc cataagtccc
    24181 tctctttgta ttctttcaag agaatttacc cctacttgat aaccatactt aagtccaaat
    24241 attctaaggg caaatgctgg tatattgtat aatagtaaga ataatagtgg tccaaagata
    24301 ttaccttctt ttgcaagtga tactcctact cctgctgcta taatcctaaa tgttccccag
    24361 aataatgaat caccaattcc tgcaagaggt cccataagag ctgttttaac agcactaatt
    24421 gaactttcat caaattctgg attattagca ttctcttctt ccatagctgc tgttatacct
    24481 aatatagcag gaactactac tgtcgttgta ttaaaaatct ccaaatgcct ttctattgct
    24541 tttgcttgat tttctttctt attgtatagc ttctttattg caggtaacat tgaataacag
    24601 taacctaagt tttgcattct ttcgtaatta aatgcacctt gaagagcaaa tgaacgccaa
    24661 aacatacttc ttaattcttt ttttgttaat ttattcaaag tcaatctctc cttccgttaa
    24721 agtattgatt gctaattgag gattttgatt attttctttg ttattgatat aaaagtttag
    24781 tacaaatgct acacatactc caaataaagc tatagcagtt atatctaatc caccataaga
    24841 tgctaacaaa aaccctataa agaaaaatac agacatcttt tttgaaagta acatatccat
    24901 aagcaatgca aaacctactg ctggcaacaa tgttcctgca acacccagtc cgtctaatat
    24961 aactttaggt attgcattta tcaatgctgt aactttatca gcaccaatca taattgctaa
    25021 aaaggttgga actgatgtac ttaaaaagaa taatattact ggcaaccaca tcattatggt
    25081 aactttctta aaatcagctt gtttagcata gaaaactgcc ttttgagaaa agtacgaatt
    25141 tattattcta actaatactc caagagattg tgctaagact gctactggta ctgctactgc
    25201 taaagcaact tctgcaccat tacctgataa tatagcaaaa gctgtaccta atacacctcc
    25261 tgttactaca tctggaggag tagctgcacc tattcctgca attcccatcc aaattaattc
    25321 aagctgtgca ccaatcataa ttcctgattt tatgtctcct aaaactaatc ctactaacat
    25381 acctgtaact attggcctat caagcataga ttgaccaaat atacgcccat ctagtatacc
    25441 aactcctgct ataatagcta acatagtagc ttgtactaac atattgaacc tccattttaa
    25501 cttagtttaa tttacattaa attacattac atttttgatt ttgttttgat tttctaataa
    25561 ctcatttaca taaacaattc cattttttgc ttcctctaat cctgatttaa ctattagttt
    25621 tgtatcctcc tcaactaata aaagtataga taaaagcatt ggtaaattta ctcctgtcaa
    25681 taattcaaat ttgaaccctt gtaaaagtaa gtttgtacat atgtttgcca cacttccacc
    25741 aaaaacatct gttaagacaa caaattcttc actatcttta ttatttgata atatacttct
    25801 aatttctttc tcagcatcat ctgctgattt atccgttgtc atacaaaaat actcaatatt
    25861 agttaatgaa ccaattatta atttacttgt gtcaacaagc tctctggaca attctccatg
    25921 tgttgcaatt agaaattttt tcattctctt tctcctaaat tattttatta aatctgatag
    25981 ttgtttcttt ttgtctctag gtaacattct aaattctata tcaatcccta aatcacctat
    26041 ttgtttaaag ttatttatat catcatcatc aacagcaact aaatctgtaa gtttcttctt
    26101 cccttcctca tatcttaatc ctcctacatt tatttctttt aatactcctc ctgaattttt
    26161 agctacaact aaagcatctt tggtattttt tactaacact aatacattgc ttttctttga
    26221 agaagcaaat ttttctacta tttctccaga ttcctctacc ccccttatat ataaagttac
    26281 attgctaggt ttcgctaagt tcagtgacat agctttcatt tggtcatttt ttgcttcatc
    26341 atttgcaact agtattacat ttatacctag tgatgttgcc catccatatg caacctgacc
    26401 atgtatcaat ctgtcatcaa ttctcatcaa agataccatt ttccccaaat cctccatcat
    26461 acattaaaat ttttattgtt tatactctca tttatctata aaagcaaaaa gtatgccaaa
    26521 tactggcata cttttttaaa tttcattcac ttattttgta atcttgtatt ttactgtggt
    26581 agaccataga taatattata aatatatcct atctctgaaa ttggtatttg cacactataa
    26641 ctcttttcta gcacactaaa tgcatttttt attagtttta cttcactatg atggtttttg
    26701 attaacaaat ccatatcagt atactcttca atttcagttt gtctaactaa tctttcaatc
    26761 atacaactta agtgaacata taaagaaatc tttttatcat ttgaaagttt tttcttttga
    26821 atattttcta tagcattaat tccttcttct attctctcta ttatcttttt tgaatctaaa
    26881 atagttagac tatctattac tctaattaat gaaaaattct ttacaataga atcatttatg
    26941 ttctttattt tttcattttc tactactctg ctaaatattc taaaaacatc atcttctcct
    27001 tttcctgata ttatatcctc taataatata aaatctaccc cattcacatt tggattggca
    27061 gttcctacta tagccatcac atcatacatt tcaaaaactg gttctttatc tcccatagaa
    27121 acaagactat cataatcata aggtattatt ttgacatcaa ctattccaat taaactttcg
    27181 gaaagtagat gttggatttt tatagcagta cctatccctg taaaacaaga tgatattata
    27241 gcatattctt tatttttttg aggataatac aattgtatat taggtttagt ttcttcatcc
    27301 ataatttctg caatctcttc aatgttttta ccttgcatta ataaatttcc tacttctagt
    27361 gcagaaatag tggatatttg gtctataaat aacattgggc tatttatctt attctttaaa
    27421 tcttcatata taagattaag agagcccata tcaaataaca caattaaacc attctttatc
    27481 ctatctccct ctaggtatct agacatttgt tttgatacat ccgcaatagt agcatctata
    27541 ggcatatcaa tagctgtaaa tatattttta cccaacatcc gattacatac attcgccata
    27601 ctactggcag tagcatatcc atgtgctata atagtcgcat taacttgatt aaatttcatt
    27661 cttataattg ttgatttaat aaatataatc attatgatta tatctacatt attaagatac
    27721 atatccaatc tattcgttat taatgttgat attttactta ttatagagta ttctttgtaa
    27781 ctctcttttt ttaaatatat tagtaattta ttttctaaag ctttatactc atcagacaat
    27841 tgataagatt cgtccttcaa ataaagataa gacgaaaatg ccaatacatt attcccatca
    27901 tattttatat tgttattaac ttctaaattt ctaaatatat cctgcattat cgatattata
    27961 agctccataa gtacgttatt attttcatca ttatttgaaa agactaaatt gtccattagt
    28021 ctaatcatta gattagaact ctttttacta aatttttcct ttgttatttc atttttttct
    28081 agtctatcaa agtaatctaa acattttatg aaactttgat agattatatt tcttttattt
    28141 cttccatttg acatttcaat actaaattgt ttaggagaaa agataatgct tttgctttta
    28201 ccactttgga attgaaatat atctacattt tctatataaa gatttctagg aaggtcttga
    28261 agagtaacaa aaatatttcc atttcttctt tgattttttg cataagctcg tccacatgca
    28321 tattttatta catttttaca ttcacctata ttccccttga attttatctt taaaatcgca
    28381 tttaatgtaa tttgagttat ctctataggc aaaccaagta ccttactttc ttcataaata
    28441 aaatgttcta caatctttct tttttcaata gtccctcttt cttctatatt gggaacataa
    28501 atatttatgg gtattcttct taaaaaggtt tgtaaaaatt cagatagact ttctgttgta
    28561 gcaaatatta atcttaccct agcttttcta attactccat tctctccaat cctagaaaac
    28621 gtacctgtat ccataaatgt aaataacttt tcctgacctt cactatttaa tctatgtact
    28681 tcatctaaaa ataaaacacc accatctgat tgttctatta gacctgctct agattcattt
    28741 gctccagtat atgctccttt tacatgccca aataatagac tagacaataa ctccaaatta
    28801 ttataatact gcgcacagtt taacacattg tatggagaat ttttttctaa tacattactg
    28861 cttacactat atttgtatat tatcttggct aaaaaagttt ttcctactcc actttcacct
    28921 gtcagcatta ttgtaagtcc gccatttgga taaagtacag cggtttttat ttgttcgatt
    28981 acttctttta agctattatc atatcctatg atactattaa acacagtctc ttgctttaat
    29041 aatttacact catttttaag ttcatctaaa ttttcataaa tacttttttt tgtaggtcct
    29101 actacatcat ttattttatt tttaggtaaa aaaataacag gtctactgtt tatctttata
    29161 agattatcag attttactaa atcatttaat aatctacttg attgagttct acttaaatta
    29221 aactttttta ctatatcttc tgttattatg ccactttctt ttttaagttc atcaaagttg
    29281 ttaaataata cacattcatt tacaaaactt aaaatttcat ccctcatgtt caacctccaa
    29341 tacaaatatc aataatacta taatattagt atatcattta tatatttatg aaaaagataa
    29401 ttatattcct atcttgattc tcttttgtaa gtttgtaaaa tcaaactttt tataagtata
    29461 aaatctagat tttttaacat aatctcaagg ttcttatctt tccatatatc tgacatagaa
    29521 ttt
//
LOCUS       DAMJVK010000044        29358 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.044, whole genome shotgun sequence.
ACCESSION   DAMJVK010000044 DAMJVK010000000
VERSION     DAMJVK010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29358
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.044"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(142..1011)
                     /locus_tag="PC414_002042"
     CDS             complement(142..1011)
                     /locus_tag="PC414_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2464437.1"
                     /translation="MEYDMPLYRPPSEAYSLIIQVTLGCSHNKCTFCSMYKSKKFKIK
                     PLEVIKNEIDIFRRYYKHVERIFLADGDALIIPMEKLRDIILYIKEVFPECERITMYG
                     SPKSIEKKSDDELKELRKLGVKMIYLGLESGSNEVLEDIKKGFSSEQLIKVGRKVKKS
                     GIKLSATVIAGLGGTKKTHQHAVDTGKMLGAISPDYVGVLSLMVEPNTELYDLLKAGE
                     FTILEDKAVLQEIKEMIKNIDANEKIVFRSNHASNYANLKGVLPDDKQRLIDEIDYYL
                     SNQKLKREEYRRL"
     gene            complement(1108..1965)
                     /locus_tag="PC414_002043"
     CDS             complement(1108..1965)
                     /locus_tag="PC414_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2464438.1"
                     /translation="MSKDVKVKRNKKYDTFSITMELERPLLYDEVENKDDKNNNRKFN
                     IKNKIFKGIGILFVLILLGFFIWINKTYEPQKLAKEALVSNSKVEVTVNENISFTPKG
                     RKVSKGLILYPGAKVEIESYAPLARKIAESGYEVVIVKMPLNLAILGTNKAQKVMDSY
                     NNIEHWVIGGHSLGGVAASNFARDNKLIEGVVFLASYPMGDELKELGKKVISIWGSKD
                     GVVNFKSLVESKQKLPDDTTYVEIEGGNHAQFGDYGKQKGDNDAIISQEKQLNITANS
                     IIKFLKNIS"
     gene            complement(2180..4198)
                     /locus_tag="PC414_002044"
     CDS             complement(2180..4198)
                     /locus_tag="PC414_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDF2464439.1"
                     /translation="MTKKLVISKTKLFIFIIAFISIFIYLFGSKNTLIGVGIVTAMLT
                     LLERDLTISPIKNLLKYLAINIILGILSFFAVQNMYLGVLLNFIALFIIGYVFSYDLK
                     RAVYVPFGLMYIFMVSIPVGKSEFPMRLSALAVGAVIIMIAQFVMNRNRMKNVGDKEL
                     ISICDELLEKISLLKNTINDDSSIIKSSMRKIDSCNYRINSISKNLKMVIFDNRKDDF
                     YISIRGIDIMNILFSLERISLILERYKKDSKEFEDEDIKNILEESSINSKSDVLVVAT
                     KEINYIKMCLENKDTITDKEILGFRDYVIAQDTKNINLKEIYSVLENLYEFLVDYKKV
                     KSRDEKKAERKSKIPHEFKRLSIYKKNFNLNSIRFSYAVKIALATAVAGFIMDYFHLR
                     DGRWIMLTVFSLTQPYAENCIQRSRKRIEGTFIGAVIFIVLFSIIKDSTLRSLIVLLA
                     GYINSYVVDYRKLMVCVTVSALGSAVVMGDPNVLTISRISYVALGAIIALIVNKFILP
                     YDAKTGYQHVIEMYKGIVKNIIDEVNKSIENVADVYYIKNLLLIPSLIEDRLMLINAI
                     YKDKHQEDFLENQKLLISNMYNLYINVNKNKIKDEDVEKILRDTNYISNYNVDKYDEG
                     RSVILESIINTKSLGDKIICLNLLQTLNGVKEMYRISNITKVSIKEAA"
     gene            complement(4469..5245)
                     /locus_tag="PC414_002045"
     CDS             complement(4469..5245)
                     /locus_tag="PC414_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDF2464440.1"
                     /translation="MHKETLDKLTNAAINKINLLNTSKVKYLVSSAFAGLYVGIGILL
                     IFTIGGLLTDAGSPMTKIVMGLSFAIALSLVIMTGTELFTGNNMVMSAGMLNKGVSIK
                     DTSKIWAYSWVGNLIGALVLGIIFVGTGLIDKGPVAEFFANTAASKASMPFTALFFRG
                     ILCNILVCVSVLCSFRTNSDTAKIIMIFLCLFAFITSGFEHSVANMTIYSVSLFSPTI
                     NTVTIGGAIYNLVAVTLGNIVGGALFMGLGTYILGKEKLN"
     gene            complement(5302..6273)
                     /gene="asrC"
                     /locus_tag="PC414_002046"
     CDS             complement(5302..6273)
                     /gene="asrC"
                     /locus_tag="PC414_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit C"
                     /protein_id="HDF2464441.1"
                     /translation="MIRDLNTKKVMKNAYRITKTKYETSLRVRVPGGLIDPESLAVVS
                     KIASEYGNGQVHITTRQGFEILGINMEDMEEINKIIQPVIEKMNINQSEKNAGYPAAG
                     TRNIAACIGNKVCPKAQYNTTEFAKRIEKAIFPNDLHFKVALTGCPNDCIKARMNDFG
                     IIGMALPIYEKDRCVNCGACVKKCSKISVGALKTENNKVVRDKDKCIGCGECVLNCPT
                     NAWTRDEKKYYRLAIMGRTGKKNPRLAEDFLLWVDEDSIIKIILNTYRYVEKYIDKDA
                     PGGKEHIGYIIDRTGFMEFKKWALEGVELPEITKMYENIYWSGIKYL"
     gene            complement(6286..7077)
                     /gene="asrB"
                     /locus_tag="PC414_002047"
     CDS             complement(6286..7077)
                     /gene="asrB"
                     /locus_tag="PC414_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrB"
                     /protein_id="HDF2464442.1"
                     /translation="MNPYIPVSAKILDIVKHTDKEWTFRVNCNTKGVLPGKFYEISIP
                     KYGESPISVSGYGEDYIDFTIRNVGKVTSELFNYKEGDSFFIRGPYGNGFDVSLYEGR
                     EIVVVAGGSALAPVRGIVEYFYNNKEKCEKFKLIVGFKSPKDILFADDLKRWSKKLDI
                     LVTVDGAEEGYSGNVGLVTKYIPELDMKDINNTSIVVVGPPMMMKFTVEEFLKRDLDE
                     KNIWVSYERKMCCGIGKCGHCKMDDTYICLDGPVFDYSYAKNLID"
     gene            complement(7078..8085)
                     /gene="asrA"
                     /locus_tag="PC414_002048"
     CDS             complement(7078..8085)
                     /gene="asrA"
                     /locus_tag="PC414_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrA"
                     /protein_id="HDF2464443.1"
                     /translation="MKLKLDSSKFNEGLNYLKKDYKIFAPVLIPFKGTFSDTDVIRYK
                     EVNAFEEMEFAKKANFSPKEVVLPINQVLFYFTEKEFKESDLDNKKILIFLRACDING
                     IKRLDEIYLNNGEEKDYFYKNIRDKVKFALVGCKESFRNCFCVSMDSNKTDNYSIGLN
                     IEGDTLYLDIKDSEFEVFNGEATEFNVDYVTDNLISVDVPENIDSNEMIGNSIWDEYD
                     TRCIGCGRCNFVCPTCSCFTMQDIFYKENENVGERRRVWASCQVDGYTDIAGGNSFRK
                     KQGERMRFKTMHKIHDFKKRFGYHMCVGCGRCDDACPQYISFSKCIEKINDLVISKEE
                     V"
     gene            complement(8476..9165)
                     /locus_tag="PC414_002049"
     CDS             complement(8476..9165)
                     /locus_tag="PC414_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDF2464444.1"
                     /translation="MSKLKDYNIDKVIIFNNLSTKTKNQLKEKVKLKRLQKKEILFYE
                     KDSLDSVYILLEGKVSIFKISENGERKVIFILNSGEVINDITLDASKNSTVGCEAFEN
                     SIVAYYSIEEFLEIMQNDFQLTKDIISYMERRIRRLYRQLKNSISIKVDKKLAAKLYR
                     LSREFGVCCGEWTLLNVNITVTYLADMLGCKRETVSRMIKVLQKEELIKIDNKGFYVK
                     ETELSRYFKNN"
     gene            complement(9228..10076)
                     /locus_tag="PC414_002050"
     CDS             complement(9228..10076)
                     /locus_tag="PC414_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2464445.1"
                     /translation="MSILEQLESPTFKVTKSDKLLIAYIKENIDDVFYKPISQIAKES
                     NIGEATITRFVKKMNFNGLQDFKVTLAQEISTINKKNIINKNIQNDEPALDTAKKLLS
                     SNVTTLENTVEIINSKDVHDCARLIINAKKVYFIGIGYSGIIAQDSNYKFMRIGLNCV
                     SFDSSHTMIMMSSIMEEGDLIIAISHSGETEEIIKTVKLARANNAKIISITENKNSEL
                     KDISDVHLSYVSGETVLETGSISSKLAQFFIIDLVYTQVVKELSNEAIERKIKTTNAI
                     KLFKNE"
     gene            complement(10095..10982)
                     /locus_tag="PC414_002051"
     CDS             complement(10095..10982)
                     /locus_tag="PC414_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDF2464446.1"
                     /translation="MKNYVCIDVGGTSIKYGFIREDGFIIDKSSLDTEAKEKGGEGIL
                     AKIKDIVKKYIEENEISGICISTAGMVDPVEGKILFALEELIPNYKGMQLKKEVEKEF
                     NIKCEVENDVNCAGLGEMWLGAGKDATSSICLTIGTGIGGCIIINNKLINGFSNSAGE
                     VGYMNVNGSSFQELASTSSLIKKVAKIKNLNENDLNGKIIFDLAKNNDEDCLKELDNM
                     IKSLAVGIANICYVINPEVVILGGGIMAQEKFLKPKIDKALKEVLIERVYKNTNIEFA
                     KRQNDAGMLGALYNFLNKI"
     gene            complement(11040..11843)
                     /locus_tag="PC414_002052"
     CDS             complement(11040..11843)
                     /locus_tag="PC414_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464447.1"
                     /translation="MENIDVFLLIGQSNARGLGNPKESIIPNENCFEYLSTDEIINMR
                     CELETSEGDGTIAPAFSNEWNKLTGNKVCFIHNAKDGSRIKNWNHDNNWFLNDTIEKF
                     NTGCTTLSKHYNIENKYVIWIQGESDAKYGSDVLYYKESLKKIAYRLKEECMIDKMFV
                     SLTGYWLGEDEYFIRTRRIAAAQESACSECDILCVGSKIAMQFHDRGLTIDDVHYTQE
                     GLNMLGEDLCKNIYKYHITKEKSVLSDTIDLSEARKYICELEKISKRFV"
     gene            complement(12003..12455)
                     /locus_tag="PC414_002053"
     CDS             complement(12003..12455)
                     /locus_tag="PC414_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HDF2464448.1"
                     /translation="MIFGNINHSKTYSTLHEDLLRCFEYAKDNSLIDYEKGTYTIDGD
                     DIFVNIVEYKTCEKEDRFWEAHKKYIDVHLMLDGCERIDINFIENLEQKIYEEKGDFL
                     PLDGKNNGYMELRKGDFLVCYPEDAHMTGIKVLEKENIKKAIFKVLVR"
     gene            complement(12585..14087)
                     /locus_tag="PC414_002054"
     CDS             complement(12585..14087)
                     /locus_tag="PC414_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HDF2464449.1"
                     /translation="MVGFTWIDFAILVVYLLAVLFAGLLFSKKEMKGKEFFKGDGTIP
                     WWVTSVSIFATLLSPISFLSLAGNSYGGTWILWFAQLGMFIAIPLTIKFFLPLYSRLE
                     IDTAYEYLEMRYESKGLRVLGALMFIIYQIGRMSIIMYLPSIVLAGLTGIPVNVLIIV
                     MGVIAIIYSYTGGLKAVLWTDFIQGMVLIVGVTGTLFYLIASINGGFGTVMDTLTTGH
                     KFLASNEVMFDKNILSTSAFIIFVGAGLNTFSSYVSSQDIVQRFTTTTDIKQLNKMTY
                     GNGVLSMGLATVFYLIGTCLFIYYTQNPDLAQTVQQDQVFASYIAYELPVGITGILLA
                     AIYAASQSTLSTGLNSVATSWTLDIQTIITKDISMEKQTKIAQYISLGVGILSIAVAI
                     VLANGEIKSAYEWFNSFMGLVLGVLAGIFVLGAFCKKATKMGAYVGFIVSAILVVYLK
                     YRMPEVTSWAYSLITITTSVVVGTIVSAIEAKVKAKVSLPKAETTVYYEK"
     gene            complement(14120..14986)
                     /locus_tag="PC414_002055"
     CDS             complement(14120..14986)
                     /locus_tag="PC414_002055"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HDF2464450.1"
                     /translation="MKGIYSALLVSFDKEGNINEKGLRQIIRHNIDVCKVDGLYVGGS
                     TGENFMLSTDEKKRIFEIAKDEVKEEIKLIAQVGSVNLKEAVELAKFTTDLGYDAISA
                     VTPFYYKFDFEEIKHYYNTIINSVDNRLIIYSIPFLTGVDMSLDQFGELFENEKIIGV
                     KFTAADFYLLERMRKTFPNKLIFAGFDEMMLPATVLGVDGAIGSTFNVNGVRARQIFE
                     LTKNEKISEALEVQHVTNDLITDILGNGLYQTIKLLLEEQGVEAGYCRQPMKEATDEM
                     KSRAKEIYRKYF"
     gene            complement(15071..15742)
                     /locus_tag="PC414_002056"
     CDS             complement(15071..15742)
                     /locus_tag="PC414_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDF2464451.1"
                     /translation="MLDKVKGRLIVSCQALEDEPLHSPFIMGRMAKAAMEGGAVGIRA
                     QGVEDIIEIKKVTGLPVIGIIKRNYEDSDIYITPTKKEVDELLTTGCEMIALDATNRV
                     RPNNEDLKELIKYIKENGVLVMADISNYDEAIKAQEYGVDCVSTTLSGYTPYTKTLEG
                     PDFVLMERLVKDLEIPVIAEGKVNTPQDLKKVFELGVHSSVVGSAITRPQLITEKFVK
                     AIEIN"
     gene            complement(16015..16506)
                     /locus_tag="PC414_002057"
     CDS             complement(16015..16506)
                     /locus_tag="PC414_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF2464452.1"
                     /translation="MEGNTNNKVDDKFSKVKKKFGSGKFKVELYKEKSKLNREIDDIQ
                     EKKAKIFLEMGILTYQKIREDSINDNSFDKFCDELLELDKLIYEKNMEINEMEMAESN
                     VTCECGYVGNVNDKYCAECGKKFELENECDDFIICGYCESEIDTEAEFCPCCGRKIIM
                     DLE"
     gene            complement(16528..17892)
                     /locus_tag="PC414_002058"
     CDS             complement(16528..17892)
                     /locus_tag="PC414_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF2464453.1"
                     /translation="MGEKKINNMNYSHNNYSKKTGEPTRKIKNSHITLKSKEEKICSS
                     CNKENNTKDNYCKFCGNELYEIASLRPLETKLDLKSKIKELSYHANKRGVFLTTFTTI
                     FILFIIALIFKAIITIQFNDISYLINPAHIILALNLGQISVSMSTMMGSGFINANIGL
                     LILLIIPILVLLISNLIFMRKRCRDSKTVLANSLGVGIFYGLVLAILSIFTNVKTSSH
                     SMLEYGYALEYSYEFFSVLLNGFVLGFICTYITTYKKSYEKENMYLSLFSNSIRTFIL
                     GYVLVLVILLVLTISDSSYLNELDMSSYSNGLNLFTILPQIASYMWAFANGISVTIIN
                     STVSMFTLSSSSLFGDTKLMFYAMGALSMLILLLNGYKLRFKYNTDSIRPIIVFSIYY
                     AFLMGILALFSTFILDSNINFFNTTNYGTTLIMQFKVLQAIVISFVYSFVISLIGYKL
                     NSAD"
     gene            18311..19486
                     /locus_tag="PC414_002059"
     CDS             18311..19486
                     /locus_tag="PC414_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2464454.1"
                     /translation="MNLEKIKKFFGFPLDKKHKDNVEKELCNYLYHTGKIICIIIVIS
                     QIIMMISISAREGGPFVTPRRQWYFYMYVTLFCVSSVFMFVFSYLWNKKKDCCTFYIN
                     LWIIYVTFFCLWGCVITLLDQLGGNDLSVFSYIMLAAAAFSVLKFGESVIVFGGCFLF
                     LNLFLPGIQTIPGNEFSNIVNTFFITSLAIFVSTMLYRNRVFTYYDKLLISQQYEEIS
                     HINNLLNDKVMTDDLTKMNNRRYLEDILQKKLISHANQEPITGMMIDIDYFKQFNDNY
                     GHQKGDYCLQNIAKVILDFTSSENEHAIRYGGEEFFICIFNCNEADAKLKSECLRKII
                     ESCNFERDDVPLKCVTVSIGVYTKKIGKTITLNEFISNCDKALYTAKEQGRNCVAFY"
     gene            complement(19659..21056)
                     /gene="asnS"
                     /locus_tag="PC414_002060"
     CDS             complement(19659..21056)
                     /gene="asnS"
                     /locus_tag="PC414_002060"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDF2464455.1"
                     /translation="MQKEFITVKELYREKEQYIGKTVKVAGWIRTSRVSKNFGFIELN
                     DGSFFKNMQIVFDEKVENFKEVGKLAISSSILVEGELVSTEGAKQPVEIHAKSIVVEG
                     ESDSSYPLQKKRHTLEYLRTIAHLRPRSNTFSAVFRVRSIAAYAIHKFFQERNFVYAH
                     SPIITGSDCEGAGEMFRITTMDLNDIPKTEDGKIDYTQDFFGKEANLTVSGQLNAEIM
                     ALSFRNVYTFGPTFRAENSYTGRHASEFWMIEPEIVFADLEDNMELAEDMIKYVISYV
                     LENAPEEMEFFNSFIDKGLLERLNKIVNSEFIRITYTKAVELLIESGHEFEYPVEWGC
                     DLQTEHERYITEQIYNCPVFVTDYPKDIKAFYMRMNEDGKTVRAMDLLVPGVGEIVGG
                     SQREEREDVLLDRINEMGLREEDYWWYLELRKFGTATHSGFGLGFERIIMYMTGMSNI
                     RDVIPFPRTPKNAEF"
     misc_binding    complement(21133..21366)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(21535..21729)
                     /locus_tag="PC414_002061"
     CDS             complement(21535..21729)
                     /locus_tag="PC414_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3787 domain-containing protein"
                     /protein_id="HDF2464456.1"
                     /translation="MESKKARALKGKDNKRLKSSLLVGNESTAAWADVEKLKPHSKVS
                     IPSIKGVEEAKDWVDNGSKL"
     gene            complement(22002..23675)
                     /locus_tag="PC414_002062"
     CDS             complement(22002..23675)
                     /locus_tag="PC414_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HDF2464457.1"
                     /translation="MEKSYCIIYQGDIESALQENGINRYMVLNSQLAVIYVPLDFDET
                     ILNNIIQVAWWEESEPMSSLIEITNNVNNGETITTAAETDYIYENPYNDITGRGILLA
                     VIDSGIDYLHPDFINDDGTSKVLYLWDQEANTNPPPEGFIFGSEFTRSELNIAINRND
                     GSLSQDNIGTGTLVSGILVGNGRINSQYRGITTESDLIVVKLKSYTDTYYAGRINYSV
                     SDFLAAITYVTNIARTENKPLIINLTIGVKSSAVATTSILDTFNILSSAGVVVVSGAG
                     NQGNTDIHYSGRFSSVGEVQDVIIQDGDDYALDITLNTNGPDKVGAQIISPSGEVSHD
                     IRYSPDFYIYRGKFNLENTTYAMRFIYPYITSGKENLEIRLRDIKPGVWILRLTSELI
                     INGEYDIYLPNKNLIAPDTRFLEPDSVATITMYAASDDVITVGTFNNKTDSMWIGSSK
                     GPIRGRGIKPDIVASGVDIISTYKNGTYNTGTGTGVSSSIVTGVLALLMEYLEKQDNV
                     PRLSLFTQVLKTYLILGATKLEIYTYPNVSQGYGILNLKNTIQQIANTL"
     gene            complement(23685..27083)
                     /locus_tag="PC414_002063"
     CDS             complement(23685..27083)
                     /locus_tag="PC414_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HDF2464458.1"
                     /translation="MIIINYELIVKYNGDILRLEEELGVSVEILNSSYAIITSSNEED
                     VNILLTYPEIEFIEKPFILQTQDVQSFSSTGITGFKNRTGLTGKGTIIGIIDSGIDYT
                     LPVFRDSDGRSKILYYWDQSIQGNPPEGFREGTLYTNEDINNAIDGSMYIPISTTSLH
                     GTHVAGICATIASDARIIVVRVGNIQTDIFSRSTEFMRAIKFILDRALELRMPVTLNI
                     SYGSNEGSHRGTSLFEQYIDDMCLFWKNNIVVAAGNNADKGGHKRIRLQNNITEEVEF
                     IVGEGERILNINIWPDFVDDFSVHLVNPSNNQTQAISLTSGEIRNTLGETRITGYFYP
                     IAPYSLTRRVTLQLSSNTQITPGLWKIVFEPIDIVTGNVNIYLPTSEGLNRNTRFLIP
                     TQELTVTVPGTASRVITVGSFNSRTDIVSIFSGEGDTQLGVFKPDLLAPGEDIVSFLP
                     GGTSGALTGTSMATPHVTGVCSLFMEWGIVNGNDLFLYSQKLRALLLKGARRLSNQSY
                     PNNSSGFGFLNLSDIDLYTLSNINQDLETEDIGYRSINKSFKDEENSYKFIDGYNMQI
                     HNDLENEIYISKNASRQSGILSGIDIVHTPEFEEELAGLGMSQRFFKISDSLGVLSIN
                     NTDYNSIQRVLQLPSIIRTVSTTKMTLLGEINRGTFGGVVATEEMGVNFFKNNPNINI
                     TGRGTLISIADTGIDYLHPDFIYPDGTSKIVYLWDQTKEGTPPDGFYIGTEYTREDIN
                     RAIAENDPSLSQDEVGQGTMLSGICAGLGNVNSEYAGIAEDSELIIIKLGKIDDFYNS
                     AMLFAASQYAYKKAFELGRPLVINMSLGTSSLAGLTNRSNSEKAFFTRGLCITAGAGN
                     EGNTQTHTSGIIPYVGGSVEVELELNEDEEELSLELWLNRPDKADVIIVSPTGEESKS
                     VGISNYNKVTGLFDLEGTEYSITYIYPTTFSGQQFTNVTLKNAKRGVWKIRLVGVYII
                     TGRYNLYLPNRELLKSGTRFREVDPFYTINYPAIQDDLITIGAYNTINGSLWQSSSRG
                     PTIEDRLKPDIVAPGVNIIAAYPGNTYATITGTAAASAHAAGAAAMYFQYTFVDGRYP
                     NQAYVQKIKTFMQAGARKDSNTVYPNTNSGYGLLDVRGMFDVLR"
     gene            complement(27250..28446)
                     /locus_tag="PC414_002064"
     CDS             complement(27250..28446)
                     /locus_tag="PC414_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDF2464459.1"
                     /translation="MFIEFFYLLRSRGLNVSLNEWMTLIEALDKGLCYSNFSNFYYLC
                     RMILIKSESDFDKFDAVFLEYFKGIEHQEEIPEEIMNWLKKPDIDLEEFERLEQNPNI
                     NLDKLRAKLEERIKEQDSPHNGGNYWIGTGGTSELGHSGKGQTGIRIGGNSTYGRAVL
                     EVAGERKYRDFRDDEVLNMRQFQVALKRLRQFSTRIDAPKTELDLDKTIEETCNNAGY
                     LKLFFEKPRKNTVKLLLLMDSGGSMRGYSTLCNTLFQSVSKSNHFKDVKIYYFHNCIY
                     DRLFTTPECWLSKSINTEWILKNIDKNYKVIIVGDASMSPSELLHIGGNYRGPYNYTP
                     GIEWLKRFKRKYSKIVWMNPELRDGWDSISYWYQTQRMIQSEFNMFPLTVKGLEKALK
                     NLMVSR"
     gene            complement(28467..29288)
                     /locus_tag="PC414_002065"
     CDS             complement(28467..29288)
                     /locus_tag="PC414_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HDF2464460.1"
                     /translation="MIKKEISNFRGSSDYVVSPELMASVNVAIALEKPLLIKGEPGTG
                     KTMLAQAISNELKKDLVIWNIKSTTKAQEGLYVYDTVQRLYDSQFGGEGVDDISKYIK
                     YGKLGEAFSSNQQVILLIDEIDKADLEFPNDLLWELDKMEFYINETKETVRAKQRPIV
                     IITSNAEKELPDAFLRRCIFHYIEFPDRDMMEEIVKVHFDKVEEHLLEQVMTTFYWIR
                     SLKDIQKKPSTSELIDWIQALTLSGMPIEKIEKEVPFAGILLKNNEDIESMQRHL"
BASE COUNT         9466 a         5268 c         2984 g        11640 t
ORIGIN      
        1 atattatata tgaatatacc attttgtttt ttaataaaaa atcatgctac atcaatagta
       61 agcaactatt aatgtagcat gattttttgt atctaaataa tcttagtatt tacctatcag
      121 tatagattga tatatcaaaa actatagtct tctatattct tctcttttta gcttttggtt
      181 acttagatag taatcaattt catcaattag acgttgttta tcatcaggta atacaccttt
      241 taaattagca taatttgatg catgattact tctaaataca attttttcat tagcatctat
      301 attctttatc atctctttaa tttcttgtaa cacagcctta tcctctagta ttgtaaactc
      361 tcctgcctta agtaaatcgt aaagttcagt attaggttct accattaaac taagtacacc
      421 cacataatct ggtgaaattg ctcctagcat ttttcctgta tcaactgcat gctgatgtgt
      481 ttttttagtt cctccaagac ctgctattac agtggctgat aatttaatcc cacttttttt
      541 aacttttcta ccaactttta ttaattgttc actactaaaa cctttcttaa tatcttctaa
      601 tacttcattg cttccacttt caagccctag ataaatcatt ttaacaccaa gttttcttaa
      661 ttctttaagt tcatcatcag attttttctc tatagattta ggagagccgt acatagtaat
      721 tctttcacac tctggaaaaa cttcttttat atataaaata atatctctta atttctccat
      781 tggtattatc aatgcatctc catctgctaa gaatattctt tctacatgct tataatatct
      841 tctaaatata tcaatttcat tttttattac ttctagtggc tttattttaa attttttaga
      901 tttgtacata ctacaaaacg tacatttgtt atgagaacat cctagtgtga cttgtattat
      961 aagactataa gcctcacttg gtggtctgta aagtggcata tcgtattcca tattaaaatc
     1021 ccccttgtaa taaaatattc attctattat attattaata tttactttaa ataataactt
     1081 ataaaattat attttttaaa ttttagttta agatatattc tttaaaaatt taatgatact
     1141 attggcagta atattaagtt gtttttcttg acttataata gcatcattat ctcccttttg
     1201 ttttccataa tctccaaatt gtgcatgatt tcctccttca atctcaacat aagttgtatc
     1261 atcaggaagt ttttgttttg attctacaag acttttaaaa ttgactactc catctttgct
     1321 cccccatata gatataactt tttttcctaa ttctttcaac tcatcaccca ttggataaga
     1381 agctaaaaat acaacgcctt caattaattt attatcacgt gcaaaatttg atgctgcaac
     1441 tcctcctaat gaatgacctc ctattaccca atgttctatg ttgttgtaag aatccataac
     1501 tttctgtgcc ttattagttc caagtatagc tagattaagt ggcatcttta cgattacaac
     1561 ttcatatcca ctttctgcta tttttcttgc aagtggagca taagattcta tttctacttt
     1621 agctcctgga tacaaaatta aacctttaga aacttttctt ccttttggtg taaatgaaat
     1681 gttttcattt acagttactt caactttact attgctgact agagcttctt ttgccagctt
     1741 ttgaggttca tatgttttat ttatccatat gaaaaagcca agcaaaatca atacaaatag
     1801 tatacctatt cccttaaata tcttattctt tatgttaaat tttctattat tatttttatc
     1861 atctttgttc tcaacctcat cataaagtaa aggtctctca agttccatag ttatactaaa
     1921 tgtatcatat ttcttattac gctttacctt tacatcttta ctcataaatc taacccccat
     1981 aatatttgaa tactattata gtttatccta agtaaattgt tttttcaacc aatgaaaaaa
     2041 atcaatattt taactatttt catataaaat tcataaatat atgttttttg ttttttatat
     2101 attattttct tttagtattt tataaaacaa atagccatat actttatttt aaatatatgg
     2161 ctattctata atttaatatc taagctgctt cttttattga aactttagtt atattactta
     2221 ttctatacat ttctttgaca ccatttagag tttgcagtaa gtttagacat attatcttgt
     2281 cacctagact tttagtattt ataatactct ctaaaattac acttcttcct tcatcatact
     2341 tatctacatt gtaatttgaa atataattag tatctctcaa tattttttca acatcttcat
     2401 cttttatttt atttttatta acatttatat atagattata catattactt attagtaatt
     2461 tctgattttc taaaaaatcc tcttgatgtt tatctttata tatagcattt atcaacataa
     2521 gtctatcttc aataagtgat ggtatcaata ataaattttt tatatagtaa acatcagcta
     2581 cattttctat agacttattg acttcatcaa ttatattttt aactattcct ttatacattt
     2641 ctataacatg ttgatagcct gttttagcat catatggtaa tataaactta tttactatta
     2701 atgcaataat tgctcctaat gctacatatg aaatcctgct tatggttaaa acatttgggt
     2761 ctcccataac tactgctgaa ccaagagctg aaacagttac acataccatt aattttctat
     2821 aatcaactac ataagagttt atataccctg ctaaaagaac tattaacgac cttaatgtac
     2881 tatcttttat tattgaaaat aatacaataa atattacagc tcctataaat gtaccttcta
     2941 ttctttttct agacctttgt atacaatttt ctgcgtaagg ttgtgttaat gaaaagacag
     3001 ttaacattat ccatcttcca tctcttaaat ggaaataatc cataataaaa cccgctacag
     3061 ctgtagctaa agcaatcttt actgcataag aaaatcttat agaatttaaa ttaaagtttt
     3121 tcttatatat tgatagtcgt ttaaattcat gaggtatctt tgattttctt tcagctttct
     3181 tttcgtctct tgattttact tttttataat ctactaaaaa ctcatataaa ttttctaaaa
     3241 cactataaat ttcttttaaa tttatatttt tagtatcctg agcaataaca taatctctaa
     3301 atcctaatat ctctttatca gttatggtat ctttattttc taaacacatc ttaatatagt
     3361 ttatttcttt cgttgctaca actagaacat ctgatttact atttatgcta ctttcttcta
     3421 aaatattttt tatatcttca tcttcaaatt ctttactatc ctttttgtat ctttctagga
     3481 ttaaacttat tctttctaat gaaaataaaa tattcattat gtctatacct ctaatactga
     3541 tataaaagtc atcttttcta ttatcaaaaa taaccatttt caaattttta gatatagaat
     3601 ttattctata attacagcta tctatttttc tcatactaga tttaattata gaactatcat
     3661 cattaatagt attttttaaa agactaattt tttctaataa ttcatcacat atagatatca
     3721 gttctttatc tccgacattt ttcatcctat ttctattcat aacaaattga gcaatcatta
     3781 ttataactgc tccaacagct aaagccgaca atctcatagg aaattcactt tttcctacag
     3841 gtatacttac cataaatata tacattagac caaagggtac atatactgct ctttttaagt
     3901 cataactaaa tacataccct attataaata atgctataaa atttaataaa actcctaaat
     3961 acatattttg tactgcaaaa aatgatagta ttcctaagat tatatttatt gctaaatact
     4021 tcagtaaatt tttaattgga gaaatagtta aatctctttc caagagcgtt agcatagctg
     4081 ttactatacc tacacctata agtgtatttt tacttccaaa taaatatata aatatagaaa
     4141 taaatgctat tataaaaata aacaactttg tttttgatat tactagtttc tttgtcatca
     4201 tatcccccct aaaaatttca acaagaccaa ttttatcata tttgttatct tattcaaaat
     4261 gcgctttttt acggtaggac tttaactttt tttatattta gaactatata attcatatat
     4321 aaacgtattt tttatatata taaccgatat attttcgtat gctcccatat tttttttgtt
     4381 tcaaatattt tttcaaaaat ataaaaataa ctctctcaac aatactaaaa atgcatttat
     4441 caagagagtt ataatttata caataaattt aattaagctt ttctttacct aatatataag
     4501 ttccaagacc cataaataaa gctcctccaa caatatttcc aagtgttaca gctactaaat
     4561 tataaatagc tcctccaata gtaactgtat ttattgttgg tgaaaataat gaaacactat
     4621 aaattgtcat atttgctaca ctatgctcaa agcctgatgt tataaaagca aataaacata
     4681 agaatatcat aattattttt gcagtatcac tattagttct aaacgaacac aacactgata
     4741 cacatactag aatattacat aaaatccctc taaagaaaag agctgtaaat ggcatggatg
     4801 ctttactagc tgctgtattt gcaaaaaatt cagctacagg tcctttgtct attagaccag
     4861 ttcctacaaa tataatgcct aaaaccaaag ctccgattaa attacctacc caactgtatg
     4921 cccatatttt agaagtatct tttatactta cacctttatt taacatacca gcagacataa
     4981 ccatattgtt tccagtaaat aactctgttc ccgtcattat aaccaaactt agtgctattg
     5041 caaatgataa ccccattact atcttagtca taggtgaacc tgcatcagtc aaaagtcctc
     5101 caatagtaaa tatcaaaagt atacctatac ctacataaag tcctgcaaac gcagaagata
     5161 ccaaatattt tactttactt gtgtttaata aatttatctt atttatagct gcattagtta
     5221 atttatccaa agtttcttta tgcataattc cctcttcctt ctattaaatt ttaatttatt
     5281 caaatatatg ttttactcaa tttatagata tttaattcca ctccagtata tgttttcgta
     5341 cattttagtt atttctggaa gctctacacc ttcaagtgcc cattttttaa attccataaa
     5401 tcctgttcta tcaattatat atcctatatg ttcttttcct cctggtgcat ctttatctat
     5461 atacttttct acatatctat aagtattaag tattatcttt attatgctgt cctcatctac
     5521 ccataaaaga aaatcttctg ctagtctagg gtttttcttt cctgttctac ccattatagc
     5581 taatctatag tattttttct catctctagt ccatgcattt gttgggcaat ttagcacaca
     5641 ctctccacag cctatacact tatctttatc tcttaccacc ttattatttt ctgtttttaa
     5701 tgctccaaca gatatcttag aacatttttt tacacatgct ccacaattta cacatctatc
     5761 cttttcatat attggtaaag ccataccaat aattccaaag tcattcattc tagcttttat
     5821 acaatcattt ggacaaccag ttaaagccac tttaaaatgt aaatcatttg gaaatatagc
     5881 tttttctatt cttttagcaa attcagttgt attatattga gcctttggac agactttatt
     5941 tcctatacaa gctgctatat ttcttgtacc tgctgctgga taacctgcgt ttttctcact
     6001 ttgatttata ttcatttttt caataacagg ttgtataatt ttatttattt cttccatatc
     6061 ttccatattt attcctaata tttcaaaccc ctgtcttgta gttatgtgca cctgtccatt
     6121 gccgtactca cttgcaattt tagaaactac agctaaactc tcagggtcaa taagacctcc
     6181 aggtactcta actcttaaag aagtctcata ttttgttttt gtaattctat aagcattttt
     6241 cattactttt ttggtattta aatctcttat cacaaacttc accccctaat caataagatt
     6301 tttagcatat gaataatcaa atactggtcc atctaaacat atgtaagtat catccatttt
     6361 acaatgtcca catttaccta ttccacaaca catttttctt tcataagaaa cccaaatgtt
     6421 cttttcatct aaatctcttt ttaaaaattc ttctactgta aatttcatca tcattggagg
     6481 tccaactaca actatagaag tattgtttat atctttcata tccaattctg gtatgtattt
     6541 tgttactaat cctacattac cactatatcc ttcctctgct ccatctacag taactaaaat
     6601 atcaagtttt ttactccatc tctttaaatc atctgcaaat aaaatatctt ttggtgattt
     6661 aaatcctact attaatttaa acttttcgca tttttcttta ttattataaa aatattcaac
     6721 tattcctctt acaggggcta atgcacttcc tcctgccact actacaattt ctcttccttc
     6781 ataaagggat acatcaaatc catttccata aggacctctt ataaaaaaag aatctccttc
     6841 tttataatta aacaactcgc tagttacttt tcctacattt cttattgtaa agtcaatata
     6901 atcctctcca tatcctgata ctgatattgg actttcaccg tatttaggta tagatatctc
     6961 ataaaacttt ccaggcaata caccttttgt attgcagttt actctaaaag tccattcttt
     7021 atcagtatgt ttaactatat ctagtatttt tgcacttact ggtatatatg ggttcattta
     7081 tacttcctcc ttactaatta ctaaatcatt tattttttct atacactttg aaaatgatat
     7141 gtattgcgga caggcatcat cacatcttcc acagccaaca cacatatgat atccgaatct
     7201 ttttttaaaa tcatgtattt tatgcatagt tttaaatctc attctttcgc cttgtttttt
     7261 tctaaaagaa tttccacctg ctatatctgt atatccatct acttgacaag aagcccaaac
     7321 ccttcttctc tcacctacat tttcgttttc cttatagaaa atatcctgca ttgtaaaaca
     7381 tgaacatgtt ggacatacaa aattacatct accacatcct atacatcttg tatcatattc
     7441 atcccatatt gaatttccta tcatttcatt tgaatcaata ttctctggca catctacaga
     7501 tattaaatta tcagttacat aatcaacatt aaattcagtt gcttctccat taaatacttc
     7561 aaattcacta tcctttatat ctaagtacag tgtatctcct tctatattta atccaattga
     7621 ataattatca gttttgttag aatccatgct cacacaaaaa cagtttctaa aactttcctt
     7681 acaccctaca agtgcaaact ttactttatc tctgatattt ttataaaaat aatctttttc
     7741 ttcaccatta tttagatata tttcatcaag tctttttata ccatttatgt cacaagctct
     7801 taagaaaatt aaaattttct tgttgtctaa atcgctttcc ttaaactctt tttcagtaaa
     7861 ataaaacaat acttgattta tgggaagtac aacttctttt ggtgaaaagt ttgctttctt
     7921 agcaaattcc atttcttcaa aagcgtttac ctccttgtat cttataacat cagtatctga
     7981 aaaagtacct ttaaatggga ttaaaactgg tgcaaatatc ttgtagtctt tttttagata
     8041 attcagacct tcattgaact tagatgaatc taattttagt ttcattttta tcacctttct
     8101 tatattttag ttttacttac ttgctatctt gtttaaatct taacacttta ttttctatgc
     8161 ttccgtgata attatcacag tttcaaaaat aataaaaaaa atattaatgg agtttattaa
     8221 gttctgttaa tattttttta ttatctacat tattcgattt caaatctaat cacttaaata
     8281 caatcattaa gtacaataaa actactatct actcaatttt ttgaataaaa aagaatctta
     8341 tttgtttttt tatacaattc aatagggtaa aataaaaatt aataaattag acaataaaaa
     8401 agaatctata aatagattct cttaataata tttttattta attaaaattc taataatact
     8461 cttacttaat cataattaat tatttttaaa ataccttgat aattcagttt ctttaacata
     8521 aaaaccttta ttgtcaattt taataagttc ttctttttgc aataccttta tcattctgga
     8581 aacagtctcc cttttacagc caagcatatc agctaaatat gtaactgtaa tatttacatt
     8641 tagtaaagtc cattctccac agcaaactcc aaattctcta cttaacctat ataattttgc
     8701 tgcaagtttt ttatctactt ttatggatat agaatttttc aactgtctat ataaacgtct
     8761 tattcttctt tccatataag aaataatatc ctttgtaagt tgaaaatcat tttgcataat
     8821 ttctaaaaat tcttcaatac tataataagc tacaattgaa ttttcaaatg cttcacagcc
     8881 aacagtagag tttttagaag cgtcaagtgt aatatcattt atgacttccc cactatttaa
     8941 tatgaaaatt acttttctct ctccattttc acttatctta aaaatagata cctttccttc
     9001 taaaagtata taaacactgt ctaagctatc tttttcataa aataaaattt cttttttttg
     9061 taatcttttt agtttaactt tttctttaag ttgattcttc gttttagtac ttaaattatt
     9121 aaaaattatt actttatcaa tattataatc ttttaattta gacatgtaat atctctccat
     9181 tcctaaattt ttaagagagc tctataaaac attacaaatt tcttatttta ttcattttta
     9241 aacaatttta tagcatttgt agttttaatt ttcctttcaa tagcttcatt agaaagttct
     9301 tttactacct gtgtatatac taaatcaata atgaaaaatt gtgctaattt agaagatatt
     9361 gaaccagttt ccagtactgt ttctcccgat acataagata agtgtacatc tgaaatatcc
     9421 tttaattcag aatttttatt ttcagtaatt gatataattt tagcattatt ggctcttgca
     9481 agctttacag tttttataat ctcttctgtt tcaccagaat gtgaaattgc aattattaaa
     9541 tctccttctt ccattatgga agacatcatt atcattgtat gactactgtc aaaacttaca
     9601 caattaagac ctattctcat aaatttatag tttgaatcct gtgctattat accagaatat
     9661 ccaataccta tgaagtaaac tttctttgca tttattataa gccttgcgca gtcatgtaca
     9721 tccttactat tgattatctc tactgtgttc tctaaagtgg ttacatttga acttaaaagt
     9781 ttttttgctg tatccaaagc tggctcatca ttttgtatat ttttatttat aatatttttt
     9841 ttattgatag ttgatatttc ttgtgctaaa gtaactttaa aatcctgtaa tccattaaaa
     9901 ttcatttttt ttacaaacct agttatagtt gcttcaccaa tattgctctc tttagctatt
     9961 tgtgatattg gtttataaaa tacatcatct atattttctt ttatataagc tattaataat
    10021 ttatctgatt ttgtgacttt aaaagttggt gattccaatt gttctaaaat actcattaaa
    10081 aacccttcta ttctttatat tttgtttaaa aaattatata atgctccaag cattcctgca
    10141 tcattttgtc tctttgcaaa ttctatatta gtgtttttgt aaactctctc tataagaacc
    10201 tcctttaatg ctttgtctat ttttggtttt aggaactttt cttgtgccat aatcccacca
    10261 cctaaaatta ccacttctgg atttattaca taacatatat ttgcgattcc aactgctaat
    10321 gattttatca tattatcgag ttcttttaaa caatcttcat cattattctt tgctaaatca
    10381 aaaattattt ttccatttaa gtcattttca ttcaaatttt ttatttttgc tactttttta
    10441 attagactgc tagtagatgc caattcttga aaagaacttc catttacatt catatatcct
    10501 acttcaccag cactattact aaacccattt atcagtttat tatttattat aatacatcca
    10561 ccaattccag tacctattgt taaacatata gaagatgttg catctttacc tgctccaagc
    10621 cacatttcac caagtccagc acaattaaca tcattttcaa cttcacattt tatattaaac
    10681 tctttttcta cttctttttt taattgcatg cccttatagt ttggaattaa ttcttctaat
    10741 gcaaatagta ttttaccttc aactgggtca accatacctg cagttgatat acatatacca
    10801 cttatttcat tttcctctat atattttttt actatatctt ttattttggc taatattcct
    10861 tctccacctt tttcctttgc ttcagtatca agacttgatt tatcaattat aaatccatct
    10921 tctctaataa aaccatattt tattgaagta cccccaacat ctatacaaac gtaatttttc
    10981 ataacatatc tccatacaaa ttatttttat aaattaacaa ctctatttaa aattttatat
    11041 tacacaaatc ttttacttat cttttccaat tcacagatat atttccttgc ttctgataag
    11101 tctatagtat cacttaatac acttttttct ttggttatat gatacttata aatattttta
    11161 cataagtcct ctcctaacat attaagacct tcttgagtgt aatgaacatc atcaatagtt
    11221 aatcctctgt catgaaactg catagcaatt ttgcttccta cacataatat atcacattca
    11281 ctacaagccg actcctgtgc tgctgcaatt cgtcttgttc taataaaata ttcatcttct
    11341 ccaagccaat aaccagttaa acttacaaac attttatcaa tcatacattc ttctttaagc
    11401 ctatacgcaa ttttttttag actttcttta tagtacaata catcactacc atattttgca
    11461 tcactttcac cttgaatcca aataacgtat ttattttcta tattataatg tttacttaag
    11521 gtagtacagc ctgtattaaa tttttctata gtgtcattta gaaaccagtt attatcatga
    11581 ttccagtttt ttattcttga accatctttt gcattgtgaa taaaacaaac tttatttcca
    11641 gtaagcttat tccattcatt tgaaaatgca ggtgctatag taccatcacc ttctgaagtc
    11701 tctagctcac atctcatatt tataatttca tcagtactca aatattcaaa acaattttca
    11761 tttggaatga tgctctcttt tggatttccc aatcctctag cattgctttg tcctattaat
    11821 aagaatacat ctatattttc cataagttaa ctcctttaca aaattttaag cttttaatta
    11881 ttttcaattt tctcataaaa aactaaggct gaaaatagta atctaactaa aatttacgaa
    11941 gtctcactaa tcgctgaaag ttaaatcact attttcagcc ctagtggtta tataaattat
    12001 cattatctaa ccaaaacctt aaaaatagct tttttgatat tttctttttc taaaactttt
    12061 attcctgtca tatgagcatc ttccggataa caaactaaaa aatcaccttt tctaagttcc
    12121 atgtatccat tatttttacc atcaagaggc aagaaatcgc ccttttcttc atatattttt
    12181 tgttctaaat tttctataaa atttatatct attcgttcac atccatcaag catcaaatga
    12241 acatctatat acttcttatg agcttcccag aatctatctt ctttttcaca agttttatac
    12301 tctacaatat tcacaaaaat atcatcacca tctattgtat aagtaccttt ttcataatct
    12361 attaatgaat tatctttagc atattcaaaa catctaagta aatcttcatg caatgttgaa
    12421 tatgtcttac tatgatttat atttccaaat atcataagca ctatctccct atataattat
    12481 aaagtctatt tttatacttt tatatacaaa gtatattata caatcttata gagtaactaa
    12541 attatttatc taatcactct ataagacaat ttatcattta aatattattt ttcataataa
    12601 acagtagtct cagcttttgg taaagatact tttgctttaa ctttagcttc tattgcacta
    12661 actattgtac ctaccactac agaagtcgtg attgttatta gagaatatgc ccaagaagtt
    12721 acttctggca tacggtattt taaataaaca actaaaattg ctgatactat gaatccaaca
    12781 tatgctccca ttttagtcgc ttttttacaa aatgcaccaa gtacaaatat tcctgctaaa
    12841 acacctagta ctagccccat aaaactatta aaccattcat atgctgactt tatttctcca
    12901 tttgctaata ctattgcaac agctatagaa agtataccta cacctaatga tatatattgt
    12961 gctatcttag tttgcttttc catacttata tcttttgtta tgatagtttg tatatctaaa
    13021 gtccaacttg tagcaactga attaagccct gtcgataaag ttgattgaga agctgcatat
    13081 atcgctgcaa gtagtattcc tgttataccc actggtaatt cataagctat atatgatgca
    13141 aatacttggt cttgttgaac tgtttgagct aaatctgggt tttgagtata gtatataaat
    13201 aaacaagttc caattagata aaatactgtt gcaagtccca ttgataaaac accatttcca
    13261 tatgtcattt tattaagttg ctttatatca gttgttgtag taaatctttg aactatgtct
    13321 tgactcgata catatgatga aaaagtatta agaccagctc caacaaatat tataaatgca
    13381 cttgtagata gtatattttt atcaaacata acttcattac ttgctaagaa cttgtgtcca
    13441 gttgtaagag tatccattac tgttccaaaa ccaccattta tacttgctat taaatagaac
    13501 aatgtccctg taacacctac tattaatacc attccttgta taaaatcggt ccacaagaca
    13561 gcctttaatc cacctgtata tgagtaaatt attgctatta cacccataac tattatcagt
    13621 acatttacag gtattcctgt aagaccagcc agaactattg atggaagata cattatgata
    13681 gacatacgtc ctatttgata tattataaac atcaaagctc caagtactct aagacctttg
    13741 ctttcgtatc tcatttctaa gtattcatat gctgtatcta tttctaacct actatataga
    13801 ggtaagaaaa attttattgt caaaggtatt gctataaaca tacctaattg tgcaaaccac
    13861 aaaatccaag taccaccata agaatttcct gctaatgata aaaatgatat tggacttaat
    13921 aatgtggcaa atattgatac tgatgtaacc caccacggaa tagttccatc acctttgaag
    13981 aactcttttc ccttcatttc cttttttgaa aataacaatc ctgcaaataa aactgccaat
    14041 aagtaaacca ctagtattgc aaaatctatc caagtaaatc ctaccatttg actaatcctc
    14101 ccatactaaa agtattttat taaaaatact ttctgtatat ttcttttgct cttgatttca
    14161 tttcatcagt tgcttctttc ataggttgtc tacagtatcc agcctcaaca ccttgttctt
    14221 caagtagtaa tttaattgtt tgatataagc catttcctaa tatatctgtt attaaatcat
    14281 tagtcacatg ttgtacttca agtgcttctg atattttttc attcttagtt aattcgaata
    14341 tttgtctagc tctgacaccg tttacattga atgtagaacc aattgcccca tctacaccta
    14401 aaactgtagc tggtaacatc atttcatcaa atcctgcaaa tattaattta tttgggaatg
    14461 tttttctcat tctctctaat aagtagaaat ctgctgctgt aaacttaaca cctattattt
    14521 tttcattttc aaataattct ccaaactggt ctaaactcat atcaacacca gttaaaaatg
    14581 gtatagagta tattattaat ctattatcta cagaatttat tattgtatta taatagtgtt
    14641 ttatttcttc aaaatcaaat ttatagtaaa aaggtgtaac agcacttatt gcatcatatc
    14701 ctaaatcagt tgtaaattta gcaagttcta cagcttcttt taaattaact gaaccaactt
    14761 gagctattaa ttttatttct tctttcactt catctttagc tatttcaaat attcttttct
    14821 tttcatcagt tgaaagcatg aagttttccc ctgtacttcc tcctacataa agaccatcta
    14881 ctttacaaac atctatattg tgtctaatta tttgtcttaa ccctttttca tttatatttc
    14941 cttccttatc aaatgaaact aataatgctg aataaattcc cttcatatct gttgttctca
    15001 tatctttacc tccaatttta attttcaaat atagttaaat ataataattt tttaatttat
    15061 aaattaattt ttaattaatt tcaattgctt taacaaattt ttctgttatt aattgtggtc
    15121 ttgttattgc tgaacctact actgatgaat gaacacccaa ctcaaagacc ttttttaaat
    15181 cttgaggagt atttaccttt ccttcagcta ttacaggtat ttctaaatct ttaactaatc
    15241 tttccattaa tacaaaatct ggaccttcta atgttttagt atatggagta tatccagata
    15301 gtgtagttga aacgcaatct acaccgtact cttgtgcttt gatagcctca tcataatttg
    15361 aaatatctgc cattactaga acaccgtttt cttttatata ctttataagt tcttttaagt
    15421 cttcattatt tggtctaact ctattggtag catctaatgc tatcatttca caccctgttg
    15481 ttaaaagttc atcaacttct tttttagttg gcgttatata tatatctgaa tcttcatagt
    15541 ttctctttat aatacctata acaggaagtc cagtaacttt ttttatttca ataatatctt
    15601 caactccttg agctctaatt ccaacagctc caccttccat agcagcctta gccattctcc
    15661 ccattataaa aggactatga agaggttcat cttctaaagc ttggcatgat actattaatc
    15721 ttccctttac cttatctagc attttttaaa ttacctcgtt tctgaaattt attttcttga
    15781 tttatgtatt aataataaca tgaaaacttt ttctttgcaa tattttttca tatttttttc
    15841 tcgcatctat atttttactg atttatttta tatattagct tgttttttac atttaaaaga
    15901 aattttattt catttttttg gtgtttattt tcattttata taactactgt tataagtcca
    15961 tttaaataat ttcaataaaa aaacccatgt atactataca taggtttccc aatattattc
    16021 taaatccatt ataatttttc ttccacagca tggacaaaat tcagcctctg tatctatctc
    16081 actttcacaa taaccacaaa ttataaaatc atcacattca ttttccaatt caaatttttt
    16141 accacattca gcacaatatt tatcatttac atttccaaca taaccacact cacaagttac
    16201 attactttct gccatttcca tttcattaat ttccatattt ttttcataaa ttaacttatc
    16261 aagttctagt aactcatcac agaatttatc aaaactatta tcattaatag aatcttctct
    16321 aattttttga tatgtcagta ttcccatttc caagaatatc tttgctttct tttcttgaat
    16381 atcatcaatc tctctattta attttgattt ttctttatat aattcaactt taaattttcc
    16441 actaccaaac tttttcttaa ccttagaaaa tttatcatct actttattat tagtatttcc
    16501 ttccacagaa attccctcca atcatattta atcagctgaa tttagcttat atcctattaa
    16561 agatatcaca aaactatata caaatgaaat tacaatagct tgtaagactt taaattgcat
    16621 tattaaagtt gttccataat tagttgtatt aaagaaatta atattactat ctagtataaa
    16681 tgtactaaat aaagccaata ttcccatcaa aaatgcataa tatatactaa atactattat
    16741 aggtcttata ctatctgtat tatacttaaa tcttagcttg tatccattta ataataatat
    16801 taacatagac aatgctccca ttgcataaaa cattaattta gtatctccaa ataaagatga
    16861 agaacttaaa gtaaacattg atactgttga gtttattatg gttactgaaa ttccatttgc
    16921 aaaagcccac atataactag ctatttgtgg gagtatagta aataaattta atccatttga
    16981 ataactactc atatctagtt catttaagta actactatca gatatagtta gtaccaatag
    17041 tattactaat actaaaacat aacctaatat gaatgttctt atagaatttg aaaaaagaga
    17101 taaatacata ttttcctttt cataactttt tttataagta gttatgtatg tacatataaa
    17161 ccctaataca aatccattta aaagaacact aaaaaattca taactatatt ctaatgcata
    17221 tccatattct aacatactat gtgaactagt ttttacatta gtaaaaatag ataaaatagc
    17281 taaaactaat ccataaaaaa ttccaactcc tagagaattt gctaatacag tttttgaatc
    17341 tctacatctt tttctcataa aaattaggtt tgatattaat aataccaaaa tagggattat
    17401 aagtaatatc aacaaaccta tattggcatt tataaaccca gaacccatca tggtagacat
    17461 agatacacta atttgaccca agttgagagc taggattata tgtgctggat ttattaaata
    17521 actaatatca ttaaattgaa tagttataat agctttaaat atcaatgcaa taataaacaa
    17581 tataaatatg gttgtaaatg ttgttaaaaa tacacctctt ttatttgcat gatatgaaag
    17641 ctcttttatt ttagatttca agtctagttt tgtttcaagt ggtcttaatg atgctatttc
    17701 ataaagttca tttccacaaa atttacagta attatcctta gtattatttt ctttattgca
    17761 actagaacaa attttttctt ctttactttt aagagtgata tggctatttt ttatttttct
    17821 agttggttca cctgtctttt tggaataatt attatgtgaa taattcatat tatttatttt
    17881 cttttcaccc attttttccc tccaagtttt actattgtaa attatcaaat ataactatat
    17941 catataaaac ttaattatac cttaattgga tatatttatc tattttattt gtttattgct
    18001 gttgtttttt aacaggtctt gatatttaaa tagtttgtta atgcgaactt tattatttat
    18061 attttattat gcttaattta aatttattct attctttatt ttcagtagta aactatctgt
    18121 aaatcttaat tataataaag tattaatcaa taaagttact cgacattatc taataaattt
    18181 tatctatcac aattttagat tgttacacac aatatatttt gatataatta aatcataaac
    18241 gaagtattcc aaatatctaa tgatagaaag gaatacttat ttttatgctt tatttttaat
    18301 ggaggcttat atgaacttag aaaaaataaa aaaatttttt ggatttcctc tagacaaaaa
    18361 acacaaagac aatgttgaga aagaactctg caattatctt tatcatacgg gtaaaatcat
    18421 ttgcataata atagtaatat ctcaaataat tatgatgata tcaatctcag ctagagaagg
    18481 aggccctttt gtcacaccaa gacgccaatg gtatttttat atgtatgtaa cactattttg
    18541 tgtatcttct gtctttatgt ttgtattttc ttacctttgg aataaaaaaa aggattgctg
    18601 tacgttttat ataaatttat ggataattta tgtgactttt ttctgcctat ggggttgtgt
    18661 aattactcta cttgaccagt taggtggaaa tgacctaagt gtatttagtt atattatgtt
    18721 agcagctgca gctttctcag tattaaaatt tggagaatca gtaatagttt ttggaggatg
    18781 ctttttgttt cttaatttat tcttacctgg gattcaaact ataccaggca atgagtttag
    18841 taatattgtc aacacatttt ttataacatc ccttgccatt tttgtttcta caatgctata
    18901 tagaaatcgt gtatttactt actatgataa acttttaatt agtcaacaat atgaagaaat
    18961 atctcatata aataatttac ttaacgacaa agttatgaca gatgacctta ctaaaatgaa
    19021 caaccgtagg taccttgaag atattctaca aaaaaaatta atcagccatg caaatcaaga
    19081 acctataact ggaatgatga tagatataga ttattttaaa caatttaatg ataattatgg
    19141 acatcaaaag ggagactatt gtctacaaaa tattgccaaa gtaattttag attttacaag
    19201 ttctgaaaat gaacatgcta tacgatatgg tggtgaggaa ttttttatat gtatttttaa
    19261 ctgtaatgag gcagatgcta aacttaaatc agaatgttta cgaaaaatta tagagtcctg
    19321 caattttgag cgtgatgatg ttcctttaaa atgtgtaact gttagtattg gagtttatac
    19381 aaagaaaatt ggaaagacaa taacattaaa tgagtttata agtaattgtg ataaggccct
    19441 atatactgca aaagaacaag gacgtaattg tgttgcattt tattaaaagg ttacatacta
    19501 ttacataaac ctataagtat tatatcgcaa aataaaagta atcaaattat atatttatag
    19561 tacaataaaa ataacttgca tatctctaaa atagataaat atgcaagtca ttttagtgtt
    19621 tatacttaaa gttatataag ttttattata tataattatt aaaattcagc atttttagga
    19681 gttcttggga aaggtataac gtctcttatg ttagacatac ctgtcatata cataattatt
    19741 ctttcaaatc caagaccaaa tccagagtga gtagcagtac caaactttct aagttctaaa
    19801 taccaccaat aatcttcttc tcttaatccc atttcattaa ttctatctaa caatacatct
    19861 tctctttctt ctctttgaga acctcctacg atttctccaa ctccaggaac tagtaaatcc
    19921 attgctctaa cagttttacc atcttcattc attctcatat agaaagcttt tatatctttt
    19981 ggataatctg taacaaacac tggacaatta tatatttgct ccgttatata tctttcatgc
    20041 tctgtttgaa ggtcacatcc ccactcaaca ggatactcaa attcatgccc agattcaatt
    20101 aataattcca ctgctttagt atacgttatt ctaataaact cagagtttac tatcttattt
    20161 aatctttcta acaatccttt atctataaaa ctattaaaaa attccatttc ttcaggagca
    20221 ttttctaata catagcttat aacatatttt atcatatctt ctgcaagttc catgttatct
    20281 tcaaggtctg caaaaactat ttctggctct atcatccaaa attctgaagc atgtcttcct
    20341 gtataagagt tttcagctct aaatgtaggt ccaaaagtat aaacgtttct aaaagaaagt
    20401 gccattatct cggcatttaa ttgaccacta actgttaaat tagcctcttt tccaaagaaa
    20461 tcttgagtat agtctatttt gccatcttca gtttttggaa tatcatttaa atccatagtt
    20521 gttattctaa acatctctcc tgcaccttca cagtcactac ctgttatgat tggagaatgt
    20581 gcatatacaa agtttctttc ttggaagaat ttgtgtattg cataggcagc tattgatctt
    20641 actctaaata cagctgaaaa agtattactt cttggtctta aatgtgctat cgttcttaaa
    20701 tattcaagcg tatgtctctt cttttgtagt ggatacgaac tatctgattc cccttctaca
    20761 actatgcttt ttgcatgtat ttcgactggt tgtttagccc cttctgtaga tactaactct
    20821 ccctctacaa gtatagatga gcttattgct aattttccta cctctttaaa attttctact
    20881 ttctcatcaa atactatctg catattttta aagaagcttc catcatttag ctctataaat
    20941 ccaaaatttt tagatactct agatgttctt atccatccag ctacttttac agtcttccct
    21001 atgtactgtt ctttttctct gtacaattct tttactgtta taaattcctt ttgcatttta
    21061 aatcctccta gtataattgt tattaatttc ttttaaatat aatttcaata aaaaaagccc
    21121 ttcatcccta tataaaggga cgaaagacta ttactctcgc ggtaccaccc tagttgtcat
    21181 aatgacctct tagttaagca atgcttaaag cctataacgg tgccttccgt ctaagtctac
    21241 ttactaaata agctttcgat tagaaactcc aagatgttct tcaatataaa cctcgtattg
    21301 agctttcacc atcctcaact cgctagaact acattttata tctactcttc tcttcacagt
    21361 atttatcttt tattatttct gctaattaac aaaattatac caatttataa atttttagtc
    21421 aataaattta tataattgat actgattgtt atttaaaata taataattct accttacata
    21481 atgataataa atacttatct tcattacttc tttattactt caatttccaa aattttataa
    21541 tttacttcca ttgtcaaccc agtcttttgc ttcttcgaca cccttaattg aaggtattga
    21601 aactttacta tgaggtttta atttttcaac atcagcccat gctgctgtcg actcatttcc
    21661 aactaaaagt gaacttttaa gtcttttatt atctttacct tttaatgctc ttgccttttt
    21721 actttccaaa actatcacct ctattattta ttttgtaata gttttagcaa ttttacaaat
    21781 aaaattcata agtctaaatt ataattattt ttaaattaac ataatatctt ttcaatactt
    21841 aatttatacc tatttatata caaaatattt actttgtgta ttcaaaatat aaaactgttt
    21901 tttaattttc atatttattt tttatgctat ttttctactc tatctaaagt atttgataca
    21961 attaaactaa taatcaatta taattttaca taggttctta tctatagagt atttgctatc
    22021 tgttgaatcg tattttttaa atttaaaatc ccatatccct gcgatacatt tggatatgta
    22081 tatatctcaa gttttgtagc acccaatatt aaatatgttt ttaaaacttg tgtaaacaat
    22141 gataatctcg gtacattatc ctgtttttct aagtattcca tcaataaggc taaaacacct
    22201 gtgactatag aactgctcac tcctgttcct gttcctgtat tatatgttcc atttttatat
    22261 gtggatatta tatctacacc tgaagctact atatctggct taattcctct tcctctaatt
    22321 ggtcctttcg aagaaccaat ccacatacta tcagtcttat tgttaaatgt accaactgta
    22381 attacatcat cactagcagc atacatagtt atagtagcta ctgaatcagg ctctaaaaac
    22441 cttgtgtctg gagctatcaa atttttattt ggcaagtata tgtcatattc tccatttatt
    22501 ataagttctg aagtcaatct taaaatccat actccaggtt ttatatctct taatcttatc
    22561 tccaaatttt cttttccaga ggtaatatat ggataaataa atctcattgc ataagtagta
    22621 ttttctaagt taaattttcc tctatatata taaaagtctg gagaatacct tatatcatga
    22681 ctaacttcac ctgaaggaga tattatctgt gctcccactt tatctggacc attcgtattt
    22741 agtgttatat caagagcata gtcatctcca tcttgtatta ttacatcttg aacttctccc
    22801 acactgctaa atctgccaga ataatgaata tctgtattcc cttgatttcc tgcaccactc
    22861 actactacca caccagcact actcaatata ttaaaagtat ctagtattga agtagttgct
    22921 acagcacttg acttaactcc aatagtcaaa tttattatca gtggtttatt ttctgttctt
    22981 gctatgttag taacatacgt tatagctgct aaaaaatctg aaacactgta atttatcctt
    23041 ccagcataat aagtatctgt atatgatttt aatttaacta ctattaagtc tgattctgta
    23101 gtaatacctc tgtactgaga atttatcctt ccatttccaa ctaatatacc agaaactaaa
    23161 gttccagtac ctatattatc ttgactcaaa ctcccatcat ttctatttat ggctatattt
    23221 aactcacttc ttgtaaattc acttccaaaa ataaaacctt ctggaggtgg atttgtattt
    23281 gcttcttggt cccataaata taaaacttta ctagttccat catcatttat aaaatcagga
    23341 tgcaaataat ctatcccaga atctataact gctaataaaa ttcctctccc tgttatatca
    23401 ttatatggat tttcataaat ataatcagtt tcagctgctg ttgtaatggt ttctccatta
    23461 tttacattat tagttatttc aattagactg ctcattggtt cagattcttc ccaccaagca
    23521 acttgtatta tattatttaa aatagtttca tcaaaatcta aaggtacata tattactgca
    23581 agctgactat ttaaaaccat atacctattt attccatttt cttgtagggc agattctata
    23641 tcaccttgat aaattataca ataagatttt tccataaatc cctcctatct taaaacatca
    23701 aacattcctc taacatccaa gagcccatat ccagaatttg tatttggata tactgtgttt
    23761 gaatcttttc tagcaccagc ttgcataaaa gtttttattt tttgcacata tgcttggttt
    23821 ggatatctac cgtcaacaaa agtatattga aaatacattg cagcagcacc agctgcatgt
    23881 gcacttgctg ctgctgtacc agttatagtt gcatacgtgt tcccaggata agcagctatt
    23941 atattgacac ctggtgctac tatatctggt tttaacctgt cttcaatagt tggccctctt
    24001 gatgaacttt gccataaact accattaatg gtattgtagg ctcctatggt tattaaatca
    24061 tcttgaatcg caggataatt tatagtgtaa aaagggtcta cctctctaaa tctagttcca
    24121 ctttttaaaa gttctctatt tggaaggtat aagttatatc ttcctgttat aatataaact
    24181 cctactaatc gaatcttcca tacacctctt tttgcattct ttaaagtaac attagtaaat
    24241 tgctgtccag aaaaagttgt tgggtatata tatgttatag aatattcagt cccctctaaa
    24301 tcaaataaac ctgttacttt attataattt gaaattccaa cacttttact ttcttcacca
    24361 gtgggagata ctattattac atcagcttta tctggtctat ttaaccaaag ttctaatgat
    24421 aattcttcct cgtcttcatt taactctaat tcaacttcaa cagaacctcc aacatatggt
    24481 attattccag atgtatgtgt ctgtgtattg ccctcatttc ctgctcctgc cgttatacat
    24541 aaccctctag taaagaacgc tttttcacta ttactcctat tggttaaacc tgctaggcta
    24601 ctggtaccta aagacatatt tataactaat ggtcttccaa gttcaaaagc ttttttatat
    24661 gcatactgag atgcagcaaa caacattgca ctattataaa aatcatctat cttgccgagt
    24721 tttattatta ttagctcaga atcttctgct atacctgcat attcactatt tacatttcca
    24781 agccctgcac atatcccact caacatagtt ccttgaccaa cttcatcctg agatagacta
    24841 gggtcatttt ctgcgatagc tcgatttata tcttctcgtg tatattctgt cccaatataa
    24901 aaaccatcag gaggagttcc ttcttttgtt tggtcccata aatatactat ttttgaagtg
    24961 ccatctggat aaataaaatc tgggtgtaga tagtctattc ctgtatctgc aattgatatt
    25021 agagtccctc ttcctgttat attaatatta ggattatttt taaagaaatt tacgcccatt
    25081 tcttctgttg caactacacc tccaaaagta cctcgattaa tttctccaag taatgtcatc
    25141 tttgtagtcg atacagttct tatgattgaa ggaagttgca aaactcgttg tatgctatta
    25201 tagtctgtat tatttataga tagtacacct aatgaatcac ttatcttaaa aaacctttga
    25261 ctcataccta acccagctag ttcttcttca aattcaggag tatggactat atctattcct
    25321 gatagtattc ctgattgtct acttgcattt ttacttatat aaatttcatt ctctaagtca
    25381 ttatgtattt gcatattata accatctata aacttataac tattttcctc atctttaaat
    25441 gacttattaa tacttctata tccaatatcc tctgtttcta aatcttgatt tatattagat
    25501 agagtgtaca aatctatatc tgataaattt aaaaaaccaa atcctgatga attatttgga
    25561 taagattgat tacttaatct ccttgctccc tttagaagta atgcccttaa tttctgtgaa
    25621 tacaaaaata aatcatttcc atttacaatt ccccattcca taaacaatga acaaacccct
    25681 gtaacatgag gtgtagccat gctagttcca gttaatgctc cacttgtacc tcctggtaaa
    25741 aaagatacta tatcttcacc tggagctaat aaatcaggtt taaaaacacc taattgtgta
    25801 tctccttcac cagaaaatat agatactata tctgttcttg aattaaaact acctactgtt
    25861 ataactctac ttgctgttcc aggaactgtt acagtaagct cttgagtagg aatcagaaat
    25921 ctagtatttc tatttagacc ttcagaagtt ggcaaatata tatttacatt accagttact
    25981 atatctattg gttcaaatac aattttccat aacccaggag ttatctgtgt atttgaactt
    26041 aattgtagag taactcttct tgtgagagaa tacggtgcta tagggtaaaa ataaccagtt
    26101 attctagttt cacccaaagt attccttatc tcacctgaag ttagagagat tgcttgtgtt
    26161 tgattatttg atgggtttac caaatgtaca ctaaaatcat caacaaaatc tggccatata
    26221 tttatgttta atatacgctc tccttcccca actataaact caacttcttc tgtaatatta
    26281 ttttgaagcc taatcctttt atgacctcct ttatctgcat tatttccagc tgctacaact
    26341 atattgtttt tccaaaataa acacatatca tctatatatt gttcaaataa agatgttcct
    26401 ctatgtgaac cttcattact tccataactt atatttaatg taacaggcat tcttaattct
    26461 aatgccctat ctaaaataaa tttaattgcc ctcataaact ctgtacttct cgaaaaaata
    26521 tcggtctgta tatttccaac tctaaccact attattctgg catcacttgc aatagttgca
    26581 caaatacctg caacatgtgt tccatgtaag ctagttgttg aaataggtat atacatactt
    26641 ccatctattg cattatttat atcttcatta gtatataatg ttccttctct aaaaccttct
    26701 ggtggatttc cttgaataga ttggtcccaa taatataata tcttagacct tccatcacta
    26761 tctctaaaca caggtagagt ataatctatt cccgaatcaa ttataccaat tatagttcct
    26821 tttccagtta aaccagttct atttttaaat cctgttattc ctgtacttga aaaactttgc
    26881 acatcctgag tctgtaatat aaaaggcttt tctataaatt caatttctgg ataagttaaa
    26941 agtatattta catcttcttc attacttgat gttattattg catatgatga attaagtatc
    27001 tctactgata cacctaattc ttcttctaac ctcaatatat ctccattgta ttttacaatt
    27061 aattcataat ttattataat cacctcgact tctaaaatta ttattaatgt atatgttttg
    27121 tgtgagtcta aaatgtatta gactatataa ttttttaagt ttcagttata caatttataa
    27181 tagatatatt aaaaaaataa ataatgatgg tacaattttg aataaatcat accatcattt
    27241 atatttactt tatcttgaaa ccattaaatt tttcaatgct ttctcaagac cttttacagt
    27301 caatggaaac atattaaatt cactttgtat cattctttga gtttgatacc aataactaat
    27361 tgaatcccat ccatctctaa gttctggatt catccataca attttactgt actttctctt
    27421 aaacctttta agccactcta taccaggtgt ataattatat ggaccacgat agttaccacc
    27481 aatatgaagc aactcagaag gcgacataga agcatctcca acaattataa ctttatagtt
    27541 tttatctata ttttttaata tccattcagt attaatagat ttacttaacc aacattcagg
    27601 tgttgtaaat aatctatcat aaatacagtt atggaaataa tatatcttta catctttaaa
    27661 atgatttgat ttacttacag actgaaataa tgtattacat aaagtgctat accctctcat
    27721 tgaaccacct gaatccataa gcaataaaag cttcactgta ttttttcttg gcttctcaaa
    27781 aaataacttt aaatatcctg cattattaca agtctcttca atagtcttat ccaaatcaag
    27841 ctctgtcttt ggagcatcta ttctagtaga aaattgacga agtctcttta gtgcaacttg
    27901 aaattgacgc atatttaaaa cttcatcatc tctaaaatct ctgtattttc tttctcctgc
    27961 aacttcaaga acagctctac cataagtaga atttccacct atacgtatac cagtttgccc
    28021 ttttcctgaa tgtcctaact ctgaagtgcc tcctgtgcct atccaataat taccaccatt
    28081 atgaggagaa tcttgttctt ttattcgttc ctccaattta gctcttaatt tatcaagatt
    28141 tatatttgga ttttgttcta atctttcaaa ctcttctaaa tctatatctg gttttttaag
    28201 ccaattcata atctcttctg gaatttcttc ttggtgttct atacctttaa aatactccaa
    28261 aaacactgca tcaaacttat caaaatcact ttcacttttt attaaaatca tcctgcaaag
    28321 atagtaaaaa tttgaaaagt ttgaatagca cagtccttta tccaatgctt caattaaagt
    28381 catccattca tttaaagata catttaatcc tcttgacctc aataaataaa aaaattcaat
    28441 aaacataatt atccagctcc tattaactat aagtgtcttt gcatagattc aatatcctca
    28501 ttattcttaa gtagtattcc tgcaaaagga acttcttttt ctattttttc aataggcatt
    28561 ccactcaaag ttaatgcttg tatccagtct attaactctg atgtactagg ctttttttgt
    28621 atgtccttta aacttctaat ccaataaaaa gtagtcatta cttgttccaa taggtgttct
    28681 tctactttat caaaatgtac tttaactatt tcttccatca tgtctctatc aggaaattct
    28741 atataatgaa atatgcatct tcttaaaaaa gcatctggaa gttctttttc cgcatttgaa
    28801 gttataataa ctattggtct ttgtttagct cttacagttt cttttgtctc atttatgtaa
    28861 aactccattt tatccaattc ccaaagcaag tcatttggaa attctaaatc cgccttatct
    28921 atttcatcta ttaaaagaat tacttgttga tttgagctaa aggcctctcc tagtttacca
    28981 tattttatgt acttgctaat atcatctaca ccttctcctc caaattggct atcataaagt
    29041 ctttgaacag tatcatatac ataaagccct tcttgtgctt ttgtagttga ttttatattc
    29101 caaattacta aatctttttt taattcattt gaaattgctt gtgctagcat agttttacct
    29161 gttccaggtt cgcccttaat caaaagtggc ttttcaagag caatagcaac attaactgat
    29221 gccatcaatt ctggtgatac tacataatca ctacttcctc taaagttaga tatctctttt
    29281 tttatcataa atcctactcc ttttaaacag tattttctaa atgtatatag actttattac
    29341 tatttttgat ataaaaat
//
LOCUS       DAMJVK010000045        29077 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.045, whole genome shotgun sequence.
ACCESSION   DAMJVK010000045 DAMJVK010000000
VERSION     DAMJVK010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29077
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.045"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            174..2816
                     /gene="adhE"
                     /locus_tag="PC414_002066"
                     /gene_synonym="adhC"
     CDS             174..2816
                     /gene="adhE"
                     /locus_tag="PC414_002066"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDF2464461.1"
                     /translation="MEKKEKVVEKSNVEVCSPEIVNSVETLRMRLEEIRLAQKEFATF
                     TQEQVDKIFLAASTAANQQRIPLAKMAVEETGMGIVEDKVIKNHFASEYIYNAYKDTK
                     TCGVIEKDEAFGFTRIAEPVGVLSAVIPTTNPTSTAIFKSLIALKTRNGIIFSPHPRA
                     KNCTIEAARVVHDAAVKAGAPKGLIGWVDVPSIELTNVVMAEADLILATGGPGMVKSA
                     YSSGKPAVGVGPGNVPAIIDESADIKMAVSSILVSKSFDNGMICASEQAVIVPEKIYE
                     EVKKEFKYRGAHFLNKEETEKVGKVVIIDGSLNARIVGQPAHVIAKMADVEVPKTARI
                     IIGEVESVELNEPFAHEKLSPVLAMYKSKSFEDAVAKAEKLVADGGYGHTSSLYADSI
                     NHPERVEKFVNAMKTCRVLVNTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVSENVG
                     VKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVAAREFKDVMDKKKAFIVTDSFLY
                     NNGYTKKLTDLLDEMGIKHTTFFDVAPDPTLACAREGAKAMADFQPDLIIAVGGGSAM
                     DAGKIMWVMYEHPEVDFQDLAMRFMDIRKRVYVFPKMGEKAYFAAIPTSAGTGSEVTP
                     FAVITDQDSGVKYPLADYELMPNMAIIDADMMMEMPPRLTAASGVDALTHALEAYVSM
                     LRTEPADGLALQAGKIIFEYLPRAYKNGKNDKEAREKMAMASTMAGMSFANAFLGICH
                     SLAHKLGAFHHVQHGVANALLINEVIKFNCAEAPNKMGAFSQYRYPDCIQRYAEFASF
                     AGIKGSTDQEKVDNLIKAIDELKAKVGLPKTIKEAGVEESKFLERLDAMVEQAFDDQC
                     TGANPRYPLMSELKEIYLKVYYGK"
     gene            complement(3176..3517)
                     /locus_tag="PC414_002067"
     CDS             complement(3176..3517)
                     /locus_tag="PC414_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464462.1"
                     /translation="MLLILGNLNLNLDFDYRIIREENEDVDIFIDINYRSLDIDTDGS
                     NLFNSRIQFPFVRALILRLNKNNQCMTIHLLRDIDLFSAFANFEVDYTDSIINIKNQN
                     EKVILNKSIKK"
     gene            complement(3544..4215)
                     /locus_tag="PC414_002068"
     CDS             complement(3544..4215)
                     /locus_tag="PC414_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2464463.1"
                     /translation="MPILETINLGKIYGKKETSVHALKNANLKINKGEFVAIIGPSGS
                     GKSTFLHLVGGLERPSNGTIKVDGKDICCLSDKELARYRRQKVGFVFQQYNLIPVLNV
                     KENIELPLKLDNKKIDKEYIDDLINLLGLNERKNHLPNQLSGGQQQRVAIARALSAKP
                     SIILADEPTGNLDSKTTEEVMDLLKSSIKKYNQTLIIITHNENIARKADRIISIIDGE
                     LKLTL"
     gene            complement(4227..6803)
                     /locus_tag="PC414_002069"
     CDS             complement(4227..6803)
                     /locus_tag="PC414_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2464464.1"
                     /translation="MKDNLKIVLRYIKSYKARSLAIVVSIILATSLVVGIGTLSRSAQ
                     QADVDRLKRELGTDHVYFKNIKKNQLKSIKSSKDIKNLGITSYYASTDVNEKLPINIL
                     YTNKNYLTSHSKLIKGRFPKASNEVVVEKWILNSMGLKPEINQNLTFKLYQKENPETF
                     KVVGILEDRFIEKKKGQCEIFSNLNESELNDFTTYIEFNEGSDINTNIDNIIKNAMLD
                     EKSIGRNSMLIESTMKNGTLDNSSKYVAIAMSLFAGIVIYSIYVISIYQRVQEYGVLR
                     AIGSTNFRIFKLMLYELFILALIAMPIGICIGMGGAQIFNRSVGNIQFEGNINVTPFV
                     IPDKIILLSIACTILIILIISFFTYLKIRRISPIDAIRKTFGADKNVTKVNYLISKLT
                     LNMSVTKSISAKNIFRNKKGFIIIVLSMSIGGIIVIKDNYKYSFLDVQNKSTQEQTYM
                     NADFILSNVYLNEIGNNTSFKDIKGFNDNQIDKIKNIDGIDKVKTASILNTRIEVNKI
                     NRLDYYEAINSTPYYKLNPLFIKNKTTGKYTLKQKLKGYNDEMIKSLEKYLVSGNINL
                     KKMKKENLAIVYVPEISKTQKYTKTFTPGEGTPAVDIKVGDTIKVKYPKGGMDTGLYE
                     SLKDNYEYLEYEFKVGAIISYPFADNGMYSGDDGVDVITSSEYLKKLTGVDKYNLVYA
                     DLNENANKKKINNLLGKIGSEVPGTTTVDMMVEKENSDKMTSRTMIYSYGIVAIMFII
                     SVLNIINNISYNLTSRTSEFGMLRAIGISERGFKNMILYEGILYGVLSSIITIVSGII
                     IQFKMYYMQGFVSYGLGFSIDYKIYILVVVANIIVGILATYIPSRKINKISIVEAINI
                     TE"
     gene            7011..7946
                     /locus_tag="PC414_002070"
     CDS             7011..7946
                     /locus_tag="PC414_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF2464465.1"
                     /translation="MDNNFVFPIVILTLFIFSNLIMNKYKFYKELDILKRGILTFFIS
                     CFALILFMEGTKYIQLRLGYSFLLEGVLNIILFLVLIILSFYTGFKVIRLINNNNEQE
                     YKLQKLDSKINHKDNIIIKLRSQKHDYLKHLQVVYSLLNTGLDEDAKEYIMNISNSFK
                     SKNSRYGKVSYLDAIASLKYEECLEKDISFDVYIQDFMDNLEIEPNDMSSVLLNIIDN
                     AIESLSTIKKEKKYIRLHAYEDEFQYIIAIKNNGPKIHNVESIFWNGFSTKEGQDRGY
                     GLHLVKNILDKYDYSILVNSDDFLTEFVILIPKYY"
     gene            7965..8702
                     /locus_tag="PC414_002071"
     CDS             7965..8702
                     /locus_tag="PC414_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2464466.1"
                     /translation="MIKVLICDDDKIVRDDIANIAKESGYVDYVKKVKDGIEAIEFIK
                     NEKIDLLIIDIDMPYKNGIDSAEEILSIDKDIHIVFVTGFADYSLESFKVHPIDFVVK
                     PFKKEKILDSINIAIDHINSHKIANNNFIEDTLFVYKIRKQIHMINFDDIVMFEKNSR
                     AINLHTKDEDIIKFYENFDDLKKRLPPNFFMTHRQYIVNLRFIHKVIPINKSSLEIRF
                     INFQENAILNKSLEKDFLYRFYRVKRF"
     gene            complement(8887..13944)
                     /locus_tag="PC414_002072"
     CDS             complement(8887..13944)
                     /locus_tag="PC414_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HDF2464467.1"
                     /translation="MLERTRVEKNPKIIENFKNNFTKSSSKQYYDLAKSLWDCIENSS
                     YYYREPEDKFLNMIKKYYNCHYYEIKNMDELLSGPLKNALELIFTSEQVEELCQLIKR
                     KMDYTYSGGYYRKGYRSNKLVDYFRGIIRFISNYVDLIYYDFSVIDYLTLPNKVLYYD
                     SIMSDKIALEIDKGNQKVISALEEVIFGENQTALLTRQMIIGIMKSHSDKCYEMLSKL
                     LLAAKQQEGLRQQIVESLDEGTISASIYMLKTILDNNLERFSSVIRALDTWTGLGFSD
                     QKPGIIRKCLELSYKSLTDEKFRIESINSNDNLEIYFALWATATYNIYDSYSLIENLL
                     SCQEKYKKLVALYFLSHTESTYFKHSIASKNIKGDDLEILAWVITNFYVEGSVGYYYS
                     WQNRETNFDLSNTPIDKDFDARKEQFESLKHTLEILNSKTKSFSGSVFPWTWIEISSH
                     EILKCMMTLTAYDMNAQFIDVLMENCHLMSSDLRASLIVNFVNEPKTDRQREFLLECL
                     SDKSVSNRETVLSLLRKLELTDVELDKITNLLSLKTSSIRQTVIKILLEQDDSNLENI
                     ASSLIKSSNSQKCLGGLEIVNLLKDNTARQELYNKLILLIEDIDKSKKNTSQVQILID
                     NLLGKAKNDYCKENGFGIYNPENKVQIPEQFDFKDDSNSSSKLNKIISNISLSTREDS
                     VSVATKKEAKKILDTDINKIIKVYEKFDELITENADYEYEVERWSGNVDKVLLGYSTY
                     NLTPIKYKVSNDTLDNYPLAELWRETSKKLDLDYKLILDILFYSISTCGYNETEKSWF
                     SSIYSNVLDSKKQNKIYTKLEKLKFNGLTRKLLRVLLNEFDHKEIFNYCLEIYYKLRN
                     LLTIEQCKKQRVEFTKNYYRQNEYCPALNAPILNYWLDKAHSFIYDDISFTKFFYTTF
                     SNYQLLEYKFPFYTSINVFARAHELGLMTEEQVNREMISGYNASNIIRTLTNPRWAGD
                     TLEKYPWLKDNLKTVTNRIIEIESRRGDMPTEVSNLANNIQYFEGINYFVNILVALGK
                     ETFQRGYSYGSNYSKKDVLSSLLKNCYPTKEDDSKKLKSLLKNTDITELRLIESAMYA
                     PQWVNIIEKYLNWKGLKSTIWYFHAHINEGFSAQKESEVALFSPISPRSFIDGAFDVD
                     WFLDSYKTIGEERFNILYKSAKYITSGGNSHRRAQLYADATLGKLDKDMLMKEIETNR
                     NQDKLRSFSLLPMKKGDLKEATFRYEFIQKFAKESKQFGAQRRESESKACTIALENLA
                     RSAGFGDVNRMMWALETEKLNEVAHLFKPKDIDGTSIWIDVDENGKASTKVQKGNKSL
                     KSIPKAIAKHPYVLELKSVQKDLKSQYSRAKQSLEQAMKNEIVFTVKEIANIYKNPVI
                     CPLIKNLVWVCDDNIGYVYNDKSKFFICNIEGETYKLTAKNKLKLAHPVHLFESGKWS
                     EFQHDLFSKQIVQPFKQVFREYYRINSDELSEKTISRRYAGHQIQPKKTVALLRNQLW
                     TVDYEEGLQKVFYKENIVAKMFALADWFSPADIEPPTIETVEFFDRKTYKSLELASIP
                     PILFSEVMRDIDLVVSVAHAGGVDPETSHSTVEMRIAIAKELLLLLKINNVNFEGSHA
                     LIAGNLGEYSVHMGSGIVHKKGMGALNILPIHSQSRGKIFLPFADEDPKTAEIMSKIL
                     LLAEDSKIKDPSILSNL"
     gene            complement(13969..14388)
                     /locus_tag="PC414_002073"
     CDS             complement(13969..14388)
                     /locus_tag="PC414_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464468.1"
                     /translation="MQIYFRVKAVGKRKPMLELTPFDVSDNINCLKEAISEIVSKNVL
                     EYNEKIPEKSIINFLTNEEIESQADIGKVGFGSIYNENKQSVEKAIEVALQAFEDGIY
                     KVLINEDVIEKLDSSINLKSGDIFTFIRLTLLAGRMF"
     gene            complement(14622..14873)
                     /locus_tag="PC414_002074"
     CDS             complement(14622..14873)
                     /locus_tag="PC414_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464469.1"
                     /translation="MNRYTKIINMMGSYYTKDFEKEKKNVIKVREVKEDTVRKFFLQG
                     DCEVLVVFEDTGKEILIDDFSPEEDIKKYLGAKFINKKR"
     gene            14962..15693
                     /locus_tag="PC414_002075"
     CDS             14962..15693
                     /locus_tag="PC414_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HDF2464470.1"
                     /translation="MTSIKNKDILNCIDYSIKNKLFDKLNDVYKSLPVGNCSGCGNCC
                     MESVGINLIEFLNIFCYLEDRVDLKKSCISKILDYYFEEYTRKNPCPFKDTNNRCLIY
                     EVRPLNCRMFGHWKKEDYNKNLDNVIEKNNNYRELMKKQYGFEINDEVVNYRIDYCDR
                     FIPNKDYLSKSKRLSFFDELMILDSNIYSSGNIDIDFRDRGIVEYFIESLLYRNLAYN
                     VKIRISKEYKIRKRTIDRIKRIVLV"
     gene            15752..16663
                     /locus_tag="PC414_002076"
     CDS             15752..16663
                     /locus_tag="PC414_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2464471.1"
                     /translation="MNLTKVKGNTFFIKGGTNTGIYVFEDNTALMIDPGLCGSRPRRV
                     NKVLDENNIQVRYIINTHGHDDHYGACNQFKEYYKDVCIMSSEYDKLYIENPELFSKY
                     IVGGKSNVFMDNILKNKMLDDIKIDKSIEAGKIELNNEMFDIIEFQGHTPGSIAVLTK
                     DNVIFVGDLLIGDEILSKYDFLFLYDIQEQINSLEKLKNIDFEYLVLGHGKQVISKED
                     SYKLIEKHLNALKKYLYQIRELLVEPKSLEILLKNIINNNNLSNNYKEYHFFKSSLVS
                     VISYLVDLEEIGYILENGELLYYTKTK"
     gene            16714..17526
                     /locus_tag="PC414_002077"
     CDS             16714..17526
                     /locus_tag="PC414_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HDF2464472.1"
                     /translation="MEYEKWNEILAPYEHAVEELKIKFKNIRKEYLAKGEYSPIEFVT
                     GRTKKISSIISKLKRINAKDIETEIDDIAGIRIMCQFVEDIYAIVDLIKVRNDMTIIG
                     EKDYITNYKDSGYRSYHVIIKYPINSIAGSKEIICEIQIRTLAMNFWATIEHSLKYKY
                     DHYIPETLAERLRRASDAAFLLDQEMSEIREDIMKAQAMYQMKSIAIRDTLNRIQELY
                     DLGDTHKAMQYQRKLDRTETDKNITEILELKQEIDILLEQYKDTKVDDKVVQ"
     gene            17635..18327
                     /locus_tag="PC414_002078"
     CDS             17635..18327
                     /locus_tag="PC414_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF2464473.1"
                     /translation="MSLYAIGDLHFSTSVNKPMNIFGSNWDGHEKKIIDNWKEVVKEE
                     DMVLVLGDTSWGINLSEAKKDLDIISKLPGQKILIKGNHDYWWTTVTSLNKLYEDMRF
                     IQTNFYEYKDYAICGGRGWICPNDVKFDETDEKVYKREEHRLRLSLESARKSGHSKII
                     VITHYPPTNDKLEESLFTKLFEEYNVEKVIYGHLHGKESFKMGLKGIRNGVEYTLASC
                     DYTEFNLIKVHD"
     gene            18414..19355
                     /locus_tag="PC414_002079"
     CDS             18414..19355
                     /locus_tag="PC414_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464474.1"
                     /translation="MRWILVFIFLYLIPLVVIFRNYKNFKRSCIYSSIYVVLATTIMI
                     SNIYMSGLNKIKESMYYHNYISDETYDEKYVSNFKEKESTDLKQDNKKNEEISNNKNL
                     NNKQDRNIQNKESNEEGSNDIKEKNNSDSNVSVNNKSEKQEDNTKENNKYTSKEKNSE
                     DIKKIDMESIGIFKKEIYNIERAALVPMRECIPYTKDIAKNITKLSSIKKDVTNARNK
                     CKEVVKEYENMQIPELSKEKYTETLSNARDDVKKAYELREKSMESALKLINTKSPKYI
                     GKITEYLDLSDKQIESFKNRLNDLKEEINNSETNNIN"
     gene            19392..20549
                     /locus_tag="PC414_002080"
     CDS             19392..20549
                     /locus_tag="PC414_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HDF2464475.1"
                     /translation="MNKQSIGKLNMFFMGITQRNLDNRDFFIELEVIFKSGRKEFKGI
                     AIEEDNKYKFNFKGKSDLYTFNELLKNISKEAENYDGLVFKYVERGTIVVIEGDNKKV
                     NVKYLDNKEEVPKIDEFTASQIKNRDYYVKVGQANALLKEIGVLTKDGKIKNDKIRKY
                     NQIDHFVELIDSILKEIKDKDCITILDCACGKSYLSFVLNFYIKEVLKKNCYFIGIDY
                     SDVVIEASKNMAKNLGYKNMSFIKEDLTNYTPNRDVDLVISLHACDTATDMAIGLGIR
                     AKSEAIVVVPCCHKELLGQYRYEAMEPILKHGVFKARFADLITDGLRTLLLEGNGYDT
                     SVVEYISPLDTPKNLMIRAIKTKTNNDKALKEYKELKSQFGVEPTLEKLIY"
     gene            20701..21261
                     /locus_tag="PC414_002081"
     CDS             20701..21261
                     /locus_tag="PC414_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDF2464476.1"
                     /translation="MNKLNICIDIDGTITSPYHFLPYLNDIFNKNITEAECITHNWEE
                     LYGSGIRDVYAEFNNKYIHSYDEAKIVEGATEVISTLSKGHNLSFVTARHECLTDVTK
                     NWLSRQGFSDIDVYLLGSDYKVEKARELSCDIFIEDNPLNSVQLADDGVKVILLDTNY
                     NKDVKHQNIVRVSNWIDIEKIIKQGI"
     gene            21289..21780
                     /locus_tag="PC414_002082"
     CDS             21289..21780
                     /locus_tag="PC414_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464477.1"
                     /translation="MIDLKIENRKGKIHVKSSEIKDILELRPDFEYVQDISNTINQEN
                     ILVFDCQLTGNSFDMDDLDIEEILEELGEEIDESYFSVLFEDVRAYLKDATDEIEADL
                     QDNYLFDNLRCYFDIYNINQEFTDFKFVFLVSFKDIKISSLANLAKIVSKRQLIGASK
                     FYS"
     gene            22009..22560
                     /locus_tag="PC414_002083"
     CDS             22009..22560
                     /locus_tag="PC414_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2464478.1"
                     /translation="MQSLNEIIAENLKKIRKEKHLSLDKIAQLSGVSKSMLSQIEKCE
                     VNPTISTLKKITNGLKISFTSLMERQESDIELIQKSDIDHFIEDNGKYISYPIFPFDS
                     KRRFEIFMIEIEEGGNLDSNAELPGTQEFITVFSGEVTIKINGEDYIVSSGNSIRFKA
                     DVSHIYRNSGKGIAKMSMVVYYI"
     gene            22646..23578
                     /locus_tag="PC414_002084"
     CDS             22646..23578
                     /locus_tag="PC414_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HDF2464479.1"
                     /translation="MNNKKAVFFFCSDLKKDPVASNVIKCCEEIMDLNQTDIVIDDNF
                     VLEFKDKNNNLFHFVKTKDVISHNYKYYLPILNRHFRDYDFAGVVNWHEGENAPEHIL
                     TAHTIGDVPTGEFGNSNPRYFKNLINAIEDIKNENLLDEFTTLTEATHWSGTTYGEES
                     KLITEYSVPMLDIEIGSSSDSFNNSIAIQVLAKSLIRVFDCDEPLKTLLCVGGVHFEK
                     SFSDIIKNKEYNISIGHVLPNQWIVSGMYDDESGFEKLEKCINSIEGGIDCIVFHDKL
                     KGTYKEQCRKLGEKLNVPVFKHKILKNPKDLPIW"
     gene            23633..24496
                     /locus_tag="PC414_002085"
     CDS             23633..24496
                     /locus_tag="PC414_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDF2464480.1"
                     /translation="MILSYFALLIRVVLLAFERVVVRLLGDEEGDIYKNIASSFLFFF
                     IGGVCLLPFSIMDRVSDWSFLIPCYISSLVYSIGSVAYVTSLATGEVSLVTPINSLNS
                     LFLLLLSVIFLGEGLSLAKVAGIIIMIGGVFILKKVSSFLKNVSVIFNNLPCRLMFLY
                     IFLQSVGRVLDKAFFVQVSPILYSTILYFFVGLNLFIFLAFKKKQKIICEIFTNKKGL
                     SILSGAINGYSYLALLIALNNLELSIAEPFSQVSMIITLILAHFIFKENIKEKIPGSI
                     LILIGGWLLLL"
     gene            24743..26605
                     /locus_tag="PC414_002086"
     CDS             24743..26605
                     /locus_tag="PC414_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2464481.1"
                     /translation="MNYKKTAIDIIEQVGGSKNILALEHCSTRLRFKLIDRSKVNQDA
                     LKKVQGVMSIVNGAQFQVVIGNSVVDVYDEILKVCPLDTNDSTVKTEEKINLGTRFIE
                     FIINIFQPLIFAIAGAGILKSLLMLLAMFNILKSDSTIYTLLVAISDATFYFLPLMVA
                     VTTANVLKCNRLVAIAAVGYLLLPATTTALSEGVELFGFTIPNIAYNAQVFPAILCVS
                     FLATMEKIFNKYSPKPIRTFFVPMMSLAITVPITLTILGPLGYNVGTIFTTIILFLYN
                     KMGFLVVGLLAAFLPFMVATGMHKALIPYSINTIGKLGYEALYMTSSLAHNISESGAC
                     FAVALRTKDETLKQTALSAGISALMGITEPALYGITLQHKRAMLGVVFSSAISGTFLG
                     LFAVKGFVIVGSGLASMSMFIDPNNGNNLIFASIGFVIAILGSFIITLIIWKEDTNTV
                     ESSIGDVVEEELEEEKEVNILVTPVEGKVIDLSKVNDELFASKTLGDGVAIIPTNGNL
                     YAPCDSEVVMLFETKHAIGLRTKNGAEILIHIGINTVSMNGDGFKTFVKTGDNVKEGD
                     LLIQFDLDKISHANLDSTVMIVNNNGADYAYKVLNQSYGNVKKGSILFDVKGGI"
     gene            26608..28074
                     /locus_tag="PC414_002087"
     CDS             26608..28074
                     /locus_tag="PC414_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HDF2464482.1"
                     /translation="MRKFPEGFLWGGATAANQFEGGWNLGGKGWSVSDVAKAHFDADV
                     KDYKSNNEITTKDIEEGLAHPEDEVNYPKRHGSDFYHHYKEDIALMAEMGFKTYRMSI
                     AWSRIFPNGDDKEPNEEGLQFYDDVFDELISYGIEPLVTMSHYEPPLNIVLNYDGWYS
                     RQVINMFVRYVETICERYKNKVKYWLTFNEVDSMIRHPYTTGGLVRDRFKDKNFEEVI
                     FQAMHHQFVASAIATKICHEIIPNSKVGCMLTKLTYYPYTCRPEDVLATQQKMRSIYA
                     YSDTQVFGEYPVYLLSYFKNNNIQIVKEEHDDEIMKKYPVDFISFSYYMSSCEAADTT
                     GLDITPGNTLLAVKNPYLEMSEWGWQIDSIGLRISLIELYDRYRKPLFIVENGLGAKD
                     ILTEDKKVHDQYRINYLKEHFKCMLDAIIEDGVELWGYTSWGCIDLVSESTKQMSKRY
                     GYIYVDADDYGKGTYNRYKKDSFYWYKKVIENNSIDFK"
     gene            28098..28934
                     /locus_tag="PC414_002088"
     CDS             28098..28934
                     /locus_tag="PC414_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2464483.1"
                     /translation="MNYIIKKVLNSSVVLVHDAKYNEFILLGKGIGYGKKTGEYICGS
                     NDNQMFVPVENTKSKQFLELMDDIPVDILKITQEIIVEAEKLLNSSFNKNLYLILADH
                     FNFAIERMRKGIKITNRVFWEIKNYYPNEFKVGMVAIYMVEERLGIQLPEEEAANIAF
                     HFANAMASDGNSYDTIKYAKVIGEIINIFVFSLNRTLDKKSMHYMRFITHIKFFVERF
                     FSDSMLSSGDDLLFNQMKRSNPKEMLIALKVRDFLEKKYGKKLTNEEIAFLVVHIARV
                     KQ"
BASE COUNT        10747 a         3494 c         4654 g        10182 t
ORIGIN      
        1 ttgacaaaat attcagaaaa aaacttcttg acctaagaga gtaaaataaa taaaatctaa
       61 ggtatagaga tgaaatctat aaaattattt taagtataag ttaaaaatat agatgatgca
      121 tttaagattt ataaaaatta gagatgcata attattttag ggaggttttt agtatggaga
      181 aaaaagaaaa ggtagtagaa aaatcaaatg ttgaagtatg tagtcctgaa attgtcaaca
      241 gtgttgaaac attaagaatg agattagaag aaattagatt ggcacaaaaa gaatttgcaa
      301 catttacgca agaacaggta gataagattt tcttagctgc ttcaactgca gctaatcaac
      361 aaagaatacc tctagcaaaa atggcagtgg aagagactgg tatgggtatt gttgaagata
      421 aggttataaa aaaccatttt gcctcagaat atatctataa tgcttataaa gatactaaaa
      481 cttgtggtgt catagaaaaa gatgaagcat ttggttttac aagaattgct gagccagtag
      541 gtgttttatc agcagttata ccaacaacta atcctacatc aactgcaata ttcaaatccc
      601 taatagcact aaaaactaga aatggtataa ttttctctcc tcatccaaga gctaaaaact
      661 gtactataga agcagctaga gttgtacatg atgcagcagt taaggcagga gcaccaaaag
      721 gacttatagg ttgggtagat gttccttcga ttgaattaac taatgttgtc atggcagagg
      781 cagaccttat attagctact ggtggacctg gaatggttaa atctgcatac tcttcaggta
      841 aaccagctgt cggagttggg cctggaaatg tacctgcaat aatagatgag agtgcagata
      901 taaaaatggc tgtaagttca atcttagtat caaaatcatt cgataatggt atgatatgtg
      961 cttccgagca agctgtaata gtaccagaaa aaatatatga agaagttaaa aaagaattta
     1021 aatatcgtgg agcacatttc ctaaataaag aagaaactga aaaagtagga aaagtagtaa
     1081 taattgatgg ttcattaaat gccagaatag ttggacaacc agcacatgta atagccaaaa
     1141 tggcggatgt agaagttcca aaaacagcta gaataataat tggtgaagta gaatctgttg
     1201 aattaaatga accttttgca catgaaaaac tttctcctgt acttgcaatg tacaagtcta
     1261 aaagttttga agatgcagtt gctaaagcag aaaaattagt tgcagatgga ggctatggtc
     1321 atacatcttc attatatgca gactctataa atcatccaga gagagtagaa aagtttgtaa
     1381 atgctatgaa gacttgtaga gtattagtta atactccatc atcacaaggt ggtataggtg
     1441 atttatataa ctttaaattg gcaccgtctc ttactcttgg ttgtggttct tggggtggaa
     1501 actcagtatc tgaaaacgtt ggagtaaaac atttgcttaa tatcaagaca gtagctgaaa
     1561 ggagagagaa tatgctttgg ttcagagcac cagaaaaggt ttatttcaaa aaaggatgtt
     1621 taggtgtagc agcaagagaa tttaaggatg ttatggataa aaagaaagca tttatagtaa
     1681 cagattcttt cctatataat aatggatata ctaagaaatt aactgattta ttagatgaga
     1741 tgggaattaa gcatactaca ttctttgatg tagctccaga cccaacatta gcttgtgcaa
     1801 gagaaggagc taaagctatg gctgatttcc aaccagattt aataatagct gtaggtggag
     1861 gaagtgcaat ggatgctggt aagataatgt gggtaatgta tgagcatcca gaagtagatt
     1921 tccaagactt agctatgaga tttatggata taagaaaaag agtatatgta tttcctaaga
     1981 tgggtgaaaa agcgtatttt gctgctatcc caacttcagc aggaactggt tcagaagtaa
     2041 ctccatttgc agttataact gaccaagatt caggtgtaaa gtacccatta gcagattatg
     2101 aattaatgcc aaacatggct ataattgatg ctgatatgat gatggagatg ccaccaagat
     2161 taacagcagc atcaggtgtt gatgctttaa cacatgcatt agaagcatat gtatctatgt
     2221 taagaactga accagctgac ggtctagcac tacaagcagg taaaataata tttgaatatt
     2281 tacctcgtgc atataaaaat ggaaagaatg ataaagaagc aagagaaaaa atggcaatgg
     2341 catctactat ggcaggaatg tcttttgcaa atgccttctt aggaatatgt cactctttag
     2401 cacataaatt aggtgcattc caccatgttc aacacggtgt tgcaaatgca ttattaataa
     2461 atgaagtaat caagttcaat tgtgcagaag ctccaaataa gatgggtgca ttctcacaat
     2521 acagatatcc tgattgcata caaagatatg ctgaatttgc ttcttttgca ggtattaaag
     2581 gaagtacaga ccaagaaaaa gtagataatc ttataaaagc aatagatgaa ctaaaggcaa
     2641 aagtaggtct tcctaagact ataaaagagg ctggagtaga agaatctaaa ttcttagaaa
     2701 gattggatgc tatggttgaa caagcttttg atgaccaatg tacaggtgct aatcctagat
     2761 acccattgat gagtgaatta aaagagatat atctaaaagt ttattatggt aaataatatc
     2821 tattgtgcaa cataggttct attgggtttg tatataaatt attagatttg aatataaata
     2881 gagttttatt agagacacgt agaagtaact ataaataata gattataatt taaattctaa
     2941 atggaaatga taaaaagtca gtaatttaat ggtaatttcg aatatgtgta tttaatcaat
     3001 taactttaaa attagtcgga aaattttttt attacatata attttaaagc ataatatata
     3061 aaaaacttta tgaaaaaatt ataaaaatat tcagaaaatt ctattgacta atttatttat
     3121 catgatataa taagtttaga aaagaaaaaa attggggcct attaaggccc ccttactatt
     3181 ttttgataga tttatttaaa attactttct cattttgatt ttttatattt attatagagt
     3241 cagtataatc cacttcaaag ttggcaaaag cagagaataa atctatatct cttaacaaat
     3301 gaattgtcat acattgattg ttcttattta gccttagaat caatgctctt acaaatggaa
     3361 attgtattct agaattaaat aagttagaac catcagtatc tatatctaag ctacgataat
     3421 taatatctat aaatatatct acatcttcat tttcttctct tattattctg tagtcaaaat
     3481 ccaagtttaa atttaaatta cctaaaatca ataacattta aatcctcttt tctataaata
     3541 gttttagagt gttaatttaa gctctccatc tattatagat ataattctat ctgcttttct
     3601 agcaatattt tcattatgtg ttattattat aagtgtttgg ttatattttt ttatagaact
     3661 ttttagtaaa tccatgactt cttctgtagt tttactgtcc aaatttcctg ttggttcatc
     3721 tgctaggatt atacttggtt ttgcagataa agcacgtgca atagcaactc tttgctgttg
     3781 accaccagat aattggttgg gaagatgatt tttcctttca ttaagaccca ataaatttat
     3841 aaggtcatca atatattctt tatctatttt tttattgtct aactttagtg gtaattctat
     3901 attttccttt acatttaata caggaattaa gttatactgt tgaaagacaa atcctacctt
     3961 ttgtcttcta tatcttgcta attctttatc tgaaagacaa catatatctt taccatctac
     4021 ctttatagta ccattacttg gtctttcaag accacctact aaatgtagaa atgtactttt
     4081 cccacttcca cttggtccaa ttatagcaac aaattcgcct ttatttattt ttaaatttgc
     4141 attctttagt gcatgtacag atgtttcctt ttttccataa atcttaccta agttaatagt
     4201 ttccaatata ggcataatta ttcctcctat tctgtaatgt ttatagcttc aactatacta
     4261 attttattta tctttcttga tggaatatat gtagctagaa tacctacaat tatatttgct
     4321 acaacaacta gtatatatat tttgtaatct attgaaaatc ccaatccata tgatacaaag
     4381 ccctgcatat aatacatttt aaattgtatt ataattcctg acacgatagt aattatactt
     4441 gagagtacac catataatat tccttcataa agtatcatat ttttaaagcc tctctcagag
     4501 ataccaatag ctcttaacat tccaaattca ctagttcttg aagttaagtt ataacttatg
     4561 ttgtttatta tgttaagtac actgattatg aacattattg ctactatacc ataactataa
     4621 atcatagttc tagatgtcat cttatcagaa ttttcttttt ctaccatcat atctactgtt
     4681 gtagttccag gaacttcact tccaatttta cctaaaaggt tatttatttt tttcttgttt
     4741 gcattttcat taaggtctgc ataaactaag ttatacttat ctacaccagt taattttttt
     4801 aggtattcgc tactcgttat tacatctaca ccatcatctc cagagtacat accattatct
     4861 gcaaaaggat aacttataat agctccaact ttaaattcat attctaagta ttcataatta
     4921 tcttttagac tttcataaag acctgtatcc atcccaccct ttggatattt gacttttata
     4981 gtatcaccta cttttatatc tacagctggt gttccttcac caggagtaaa agtctttgtg
     5041 tatttctgtg ttttagaaat ttctggtaca tatacaatag ctaaattttc ttttttcatt
     5101 tttttcaagt ttatatttcc acttactaag tatttttcta atgactttat catctcatca
     5161 ttataacctt ttagcttttg ctttagtgta tatttgccag tagtcttatt ttttatgaac
     5221 agaggattta gcttataata aggagttgaa tttatagctt cgtagtaatc taatctattt
     5281 attttattta cctctattct tgtgtttaag atactagcag ttttgacttt gtcaattcca
     5341 tctatatttt ttattttatc tatctgatta tcattaaacc cttttatatc tttaaaagaa
     5401 gtattgttac caatttcatt gagataaaca tttgatagaa taaaatctgc attcatatat
     5461 gtttgttctt gagtagattt gttttgcaca tctaaaaaag aatatttata attgtctttt
     5521 atgacgatta tacctcctat actcatagat aatacaatta taatgaaccc ctttttattt
     5581 ctaaagatgt ttttcgcaga tatagatttt gttactgaca tatttaaagt taattttgat
     5641 attaaataat taactttggt tacattttta tcagctccaa aagtttttct gatagcatca
     5701 ataggagaaa ttcttcttat ttttaagtat gtaaagaaac ttataattag tattattagt
     5761 attgtacatg caattgataa gagaattatt ttatctggaa taacaaaagg tgtaacattt
     5821 atatttcctt caaattgaat atttcctaca gacctattaa atatttgtgc acctcccata
     5881 cctatacata tacctatagg cattgctatt agagcaagta tgaaaagttc ataaagcata
     5941 agtttgaaaa tcctaaagtt ggtggaacca atagctctta agacgccata ttcctgtact
     6001 ctttggtata ttgatataac atagatacta tatattacaa taccagcaaa tagactcata
     6061 gcaattgcaa cgtatttact tgaattatct aaagtaccgt ttttcatagt agattctata
     6121 agcattgagt ttcttcctat acttttctca tctaacatag cattttttat tatattgtct
     6181 atgtttgtat ttatatcact gccttcatta aattcaatat aagttgtaaa atcattcagt
     6241 tctgattcat tgagattaga aaaaatttca cattgtccct tttttttctc tataaatcta
     6301 tcttcaagga ttccaacaac tttaaaagtt tctggatttt ctttttgata taatttaaaa
     6361 gtaaggtttt gatttatctc tggttttaag cccatactat ttaaaatcca tttctcgact
     6421 accacctcat tgctagcttt tggaaatcga ccttttataa gtttagaatg actagtcaaa
     6481 taatttttat ttgtatatag tatgtttata ggtaattttt cattaacatc tgtagacgca
     6541 tagtaagatg ttatacccag atttttaata tctttgctag attttatgga ctttaattga
     6601 tttttcttta tatttttaaa gtatacatgg tcagttccaa gctctctttt caatctatct
     6661 acatctgctt gctgagcact tcttgagaga gtaccaatac caacaacgag tgatgtagca
     6721 agaattatac tcacaacaat tgctaaagac ctagctttat aactttttat ataccttagt
     6781 actattttca aattatcttt catatttacc acttcctttc ttaactaaat caattatata
     6841 tttatctaaa atatatagca aagtaagtat tttcaaattt taagtagtta attgtacaaa
     6901 ttcatctgta tttttacaag tttaattaat atatatgtaa attttaactt tttacataat
     6961 ttttttaaaa tgatataatc cctatataaa ttgtagaggg gaggggaatc atggataaca
     7021 attttgtatt tcctatagta atactaactt tgtttatatt tagcaatctt ataatgaata
     7081 agtataaatt ttacaaagaa ttagacatat taaaaagagg catattaaca ttttttatat
     7141 cgtgttttgc attgatttta tttatggagg gcactaaata tatacaatta aggttaggat
     7201 atagtttttt acttgaagga gtattaaata ttatactttt tttggtttta attattttaa
     7261 gtttttatac aggctttaaa gttataagat tgataaataa taataatgaa caggaatata
     7321 aattacaaaa actagatagt aaaataaatc ataaggataa tataattatc aaattaagaa
     7381 gtcaaaaaca tgactatttg aaacatttgc aagtagtgta ttctctatta aatactgggc
     7441 tagatgaaga tgccaaagaa tatataatga atatatcaaa tagctttaaa tcaaaaaatt
     7501 caaggtatgg aaaagtatct tatttagatg ctatagcttc attaaaatac gaggaatgtc
     7561 ttgaaaagga tatatctttt gatgtctaca ttcaagattt tatggataat ttagagattg
     7621 aacctaatga tatgagttca gtactattaa acataataga taatgcaata gagtctttgt
     7681 ctactataaa aaaagaaaag aagtatataa gattacatgc atatgaagat gaatttcagt
     7741 atattatagc tataaaaaat aatggtccta aaatacataa tgttgaaagt atattttgga
     7801 atggatttag tactaaagaa ggacaagata ggggatatgg gctacattta gttaaaaata
     7861 tattagataa gtatgattat agtatacttg taaatagtga tgatttttta actgaatttg
     7921 taatcttaat cccaaaatat tattagttag gaggagaaaa gtctatgata aaagttttga
     7981 tttgtgatga tgataagatt gtaagagatg atatagcaaa tatagctaaa gagtctggat
     8041 atgtagatta tgttaaaaaa gttaaggatg gtatagaggc aattgaattt ataaaaaatg
     8101 agaaaataga tttattgatt attgacattg atatgccata taaaaatggg attgattctg
     8161 ctgaagaaat tctttctata gataaggata tacatatagt atttgtgacg ggtttcgctg
     8221 attatagtct tgaaagcttt aaagtgcatc ctatagattt tgtagttaaa ccatttaaaa
     8281 aagaaaaaat acttgattca attaacatag caatagacca tattaactcc cataagattg
     8341 ctaataataa ttttatagaa gacacccttt ttgtatataa aataaggaag cagatacata
     8401 tgattaattt tgatgatata gttatgtttg agaaaaattc aagagcaatt aatctacaca
     8461 ctaaagatga agatataata aagttttatg agaactttga tgatttaaaa aaacgcctac
     8521 ctcctaattt ctttatgacc catagacaat acatagtaaa tctaagattt atacataagg
     8581 ttatacctat aaataaatct tcattagaga taaggtttat aaattttcaa gaaaatgcca
     8641 tattaaacaa atctctagaa aaagattttt tatatagatt ttatagggtt aaaagattct
     8701 agtgaagata gaagaaaaat actttttgtg tgatataata agtaccgtaa agataatgta
     8761 taagagaagt ttatataaaa attatttgta attatgattt aatctatata aattacagta
     8821 tagtatatat tggactttct aattttttta agaaagtcca atatatttta gagatagttt
     8881 ttatacttat aaattactta agatactagg gtctttaatc ttactatctt cagcaagtaa
     8941 tagtatctta gacataatct cagcagtttt agggtcttca tcagcgaatg gaaggaatat
     9001 cttacctcta ctttgagaat gtataggaag tatattcaaa gcacccatcc cttttttatg
     9061 tacaatacca gaacccatat gcacagaata ctcacctaaa ttacctgcta taagagcatg
     9121 agagccttca aaattgacat tgttaatttt taataataat aaaagttctt tagcaattgc
     9181 tatacgcatc tctacagtag aatggctagt ttcagggtca acaccaccag catgagcgac
     9241 acttacaaca aggtctatat cacgcataac ttctgagaat agaattggag gtatagaagc
     9301 taattctaaa cttttataag tttttctatc aaaaaattct actgtttcaa tagtaggagg
     9361 ctctatatct gctggtgaaa accaatctgc aagtgcaaac atctttgcca ctatattttc
     9421 tttataaaat actttctgta atccctcttc ataatcgaca gtccaaagct gatttctaag
     9481 aagtgctaca gttttctttg gctgaatttg gtgaccagca tatctgcgag atatagtttt
     9541 ttcagataat tcatcactgt ttattctata atactcacgg aatacttgct taaatggttg
     9601 aacaatttgc ttactaaata aatcatgttg gaactcactc cacttaccag attcaaacaa
     9661 atgaactgga tgtgcaagtt ttaatttatt ttttgccgtc aacttataag tttcaccttc
     9721 tatattgcaa ataaagaact tagacttatc attataaaca tatccaatat tatcatcaca
     9781 tacccatact agatttttaa tcaaagggca gataactgga tttttataga tattggcaat
     9841 ttctttaact gtaaatacta tttcattttt catagcttgt tctaaactct gttttgcacg
     9901 agaatactga ctctttaaat ctttttggac agatttaagt tcaagtacat atggatgttt
     9961 agcaatagct tttggtatac tctttaaaga cttgttgcct ttttgaactt tagtagatgc
    10021 ttttccgttt tcatcaacat ctatccaaat tgaagtacca tcaatatctt ttggtttaaa
    10081 tagatgagca acttcattta gtttttcagt ctcaagagcc cacatcatac gatttacatc
    10141 tccaaatcca gcacttctag ctagattttc tagcgctata gtacaagctt ttgattcact
    10201 ttcacgacgt tgtgcaccaa attgcttgct ttctttagca aacttttgaa taaattcata
    10261 acggaaagtt gcttctttta ggtctccttt tttcatagga agtaaagaga aacttctcaa
    10321 tttatcttga tttctatttg tttcaatttc tttcatcaac atgtctttat caagcttacc
    10381 aagtgtagca tcagcataga gttgtgcacg tctatgagag ttaccaccac tagttatata
    10441 tttagctgac ttgtaaagta tattgaatct ttcttcccca atagttttat atgaatctaa
    10501 aaaccaatct acatcaaatg caccatcaat gaaactacgt ggtgaaatag gggagaatag
    10561 agcaacctct gattcttttt gtgcagagaa gccttcattt atatgtgcat ggaagtacca
    10621 aattgtactt ttcagaccct tccagtttaa atatttttca attatgttta cccattgagg
    10681 ggcatacata gcagattcta tcaatcttaa ctcagtaatg tcggtatttt taagtaaact
    10741 ttttaatttt tttgaatcat cttctttagt aggatagcaa tttttaagta gagaacttaa
    10801 aacatctttt tttgaataat tgctaccata tgaatatcct ctctggaaag tttctttacc
    10861 aagagcgacc aaaatattta caaaataatt tataccttca aagtattgta tattattagc
    10921 tagattagac acttctgttg gcatatcacc tctacgtgat tcaatttcta taattcggtt
    10981 tgtaacagtt tttaaattat ctttaagcca agggtacttt tctaatgtgt cgccagccca
    11041 tctaggattt gtcaatgttc ttattatatt tgatgcgttg tatccactta tcatttctct
    11101 attaacttgc tcttcagtca ttaatcctaa ttcatgtgca cgagcaaaca catttatgct
    11161 agtatagaaa ggaaacttat attctagcaa ctgataattt gaaaacgtag tgtagaaaaa
    11221 cttagtaaaa gaaatatcat cataaataaa cgaatgtgct ttatcaagcc aatagtttaa
    11281 aataggggca tttaaagcag ggcagtattc attttgtctg taatagtttt tagtaaattc
    11341 aactctttgt tttttacatt gttctatagt caataagttt ctaagtttat agtatatttc
    11401 taaacaatag ttaaatattt ctttatggtc aaactcattt agtaaaactc ttaaaagttt
    11461 tcttgtaagt ccattaaatt taagtttttc aagttttgta tatattttat tttgtttttt
    11521 agagtcaagg acatttgaat atatagatga aaaccaactt ttttctgttt cattatatcc
    11581 acatgtactt atactataaa ataagatatc aagtattaat ttgtaatcta agtcaagttt
    11641 tttactagtt tctctccaaa gctctgctaa tggataatta tcaagagtat cattagaaac
    11701 tttatattta ataggagtca agttataagt agaataacct aaaagtactt tgtctacatt
    11761 acctgaccat ctttcaactt catattcata atctgcattt tcagtaataa gttcatcaaa
    11821 tttttcataa acttttatta ttttatttat atccgtatcg agaatttttt ttgcttcttt
    11881 ttttgttgct acagaaacag aatcctcacg agtagataaa cttatatttg agataatttt
    11941 attaagcttt gatgagctat tagaatcatc tttaaaatca aattgttctg gaatttgaac
    12001 tttgttttca gggttatata ttccaaaacc attttctttg cagtaatcat tttttgcttt
    12061 acctaacaaa ttatcaatta gtatttgaac ttgagatgta ttctttttag atttatcaat
    12121 atcctctata agtaaaataa gcttattata tagttcttgt ctagcagtat tgtcttttaa
    12181 taagtttaca atttcaagac ctccaaggca tttttgagaa tttgaagatt ttatcagtga
    12241 agaagcaata ttttccaaat ttgaatcatc ttgttcaagt aaaattttta ttactgtttg
    12301 tctaatagag cttgtcttta aagacaatag attggttatc ttatctaatt ctacatctgt
    12361 tagctcaagc tttctaagta gactcaatac cgtttctcta ttggaaacac tcttatcact
    12421 taaacattcc aataaaaatt cacgttgtct atctgtcttt ggttcattta caaaattgac
    12481 tataagtgaa gctcttaaat ctgaactcat aagatgacaa ttttccataa gcacatctat
    12541 aaattgagca ttcatatcgt atgctgttaa agtcatcata catttaagta tttcatgaga
    12601 agaaatttca atccaagtcc atggaaatac acttcctgaa aatgattttg tctttgaatt
    12661 taaaatttct aaagtatgtt ttaaactttc aaattgttct tttctagcat caaagtcttt
    12721 gtcaatagga gtgtttgaca agtcaaaatt agtttctctg ttttgccaac tatagtagta
    12781 gccaacagaa ccttctacat agaaatttgt aattacccat gctagtatct ctaaatcatc
    12841 acccttaatg tttttagatg ctatagaatg tttaaaatat gtactttctg tatgcgataa
    12901 aaagtaaaga gctacaagtt ttttatattt ttcctgacaa gaaagtaagt tttcaataag
    12961 agagtaagag tcatatatgt tgtaagttgc agtagcccat aaggcaaaat aaatttcaag
    13021 attatcattg ctattaattg attctatacg gaacttttca tcagtcaatg atttatatga
    13081 aagttctaaa catttacgta ttattccagg tttttggtct gaaaaaccaa gtcctgtcca
    13141 tgtatccaag gctcttataa cagaactaaa tcgctctaaa ttgttgtcca aaatagtttt
    13201 taacatgtat attgatgcag aaattgtacc ctcatctaaa ctctctacaa tttgctgtct
    13261 aagtccttct tgttgtttgg ctgccaaaag aagtttagaa agcatttcat agcatttatc
    13321 actatgactc ttcatgatac caattatcat ttgtcttgta agtagagcag tttgattttc
    13381 tccaaatatt acttcttcta aagcacttat tactttttga tttcctttgt ctatttctag
    13441 tgctatttta tcactcatta tagagtcata gtacaagacc ttatttggaa gagtaagata
    13501 atctataact gaaaaatcat aataaattaa atcaacatag tttgaaataa accttataat
    13561 acctctaaaa taatccacaa gtttatttga gcgataccct ttacgataat atcctcctga
    13621 atatgtataa tccatctttc ttttaattaa ttggcaaagt tcttctactt gttcagaagt
    13681 aaatattaat tctaatgcat tttttaaagg tccagaaagg agctcgtcca tatttttaat
    13741 ttcgtagtag tggcaattgt agtatttctt tatcatattt aagaatttgt cttcaggttc
    13801 tctataatag tatgaactat tttctatgca gtcccaaaga gactttgcta agtcgtagta
    13861 ctgttttgaa gaagatttag taaaattatt tttaaaattt tcaattattt taggattttt
    13921 ttctactcta gtacgttcta acatttatgt acctccaaat tatttatttt aaaacattct
    13981 tccagcaagc aaggtaagtc ttataaaagt aaatatatct ccacttttca gatttataga
    14041 ggaatcgagt ttttcaataa catcctcatt tataagcact ttgtagattc catcttcaaa
    14101 tgcctgtaac gctacctcta ttgccttttc tacagattgt ttattctcat tgtaaataga
    14161 gccaaatcca acttttccaa tatcagcttg agattcaatt tcttcattag ttaagaagtt
    14221 tattattgat ttttctggga ttttctcatt atattcaaga acatttttag atacaatttc
    14281 agagatagct tcttttaggc aattgatgtt gtcagataca tcaaaagggg taagttctag
    14341 cataggtttg cgtttgccaa cagccttaac tctaaaataa atttgcataa aatcacctcg
    14401 caaaatatta attatgtatt tagaaattta aaaatttatc tatttgttat attatatcat
    14461 tttgtgaatt attaatgtga attatggaat tattttacaa aaaatagaaa aaaagctatc
    14521 ttaaagtcta aaaaagcaat aaaagataac ttttaactat aattatatag aattataact
    14581 gtacatttat taaattttaa ttttagaatt atttttgata actatctttt tttatttata
    14641 aattttgctc ctaaatattt tttgatatct tcttcaggag aaaagtcatc aataagtatt
    14701 tctttcccag tgtcttcaaa tactacaaga acttcacagt caccttgtaa gaaaaatttt
    14761 ctaactgtat cttctttaac ttctctaact tttattacat tctttttttc tttttcaaag
    14821 tcttttgtgt aataacttcc catcatatta attatttttg tatatctatt cataaaatac
    14881 ctccatatgc gattaaattt aaaaaatatg ttataataat tgtttgtatt aacttaataa
    14941 ttataacaag gagtaataat aatgactagt ataaaaaata aagatatttt aaattgtata
    15001 gattattcca ttaaaaataa gctctttgac aaactaaatg atgtatataa gtccttgcca
    15061 gtaggaaatt gttctggatg tggaaattgt tgtatggagt cagtaggtat aaatttaatt
    15121 gagttcttga atatattttg ttatcttgaa gatagagttg atttaaaaaa aagctgtata
    15181 agtaaaatat tagattatta ttttgaggag tatacaagaa agaacccatg tccatttaaa
    15241 gatactaata atagatgttt aatatatgaa gttagaccat taaattgtag gatgtttgga
    15301 cattggaaaa aagaggatta taataaaaat ttagataatg ttattgagaa aaataataac
    15361 tatagagagt taatgaaaaa gcaatatgga tttgaaataa atgatgaagt agtaaattac
    15421 aggatagatt attgtgatag atttattcca aataaagatt atttaagtaa atctaagaga
    15481 ttaagctttt ttgatgagct tatgatatta gactcaaata tttattcaag tggaaatata
    15541 gatatagact ttagggatag aggtatagtt gaatatttta tagagtcatt actttataga
    15601 aatttagcat ataatgtgaa aataaggata tctaaagaat acaaaataag aaaaagaact
    15661 atagatagaa taaaaaggat tgttcttgta tagttttata ttagtaagta ataaattgta
    15721 tcataaatat tgtcaaaatg aggtgaagaa ggtgaatctt acaaaagtaa aaggaaatac
    15781 cttttttata aaaggtggca cgaatacagg aatatatgta tttgaagata acacagcact
    15841 tatgatagac ccagggttat gtggttcaag acctaggaga gtaaataaag tattagatga
    15901 aaataatata caggttagat atattataaa tactcatggt catgatgacc actatggagc
    15961 ttgtaatcaa tttaaagaat attataaaga tgtgtgtata atgtcatctg aatacgataa
    16021 attatatata gaaaatccag aattgttttc aaagtatatt gttgggggaa agagcaatgt
    16081 atttatggat aatatcttaa aaaataaaat gttggatgat ataaaaatag ataaatctat
    16141 agaagctgga aaaattgaat taaacaatga aatgtttgat ataattgaat ttcagggtca
    16201 tacacctggt agtatagctg ttttgacgaa ggataatgtg atttttgttg gcgatttatt
    16261 gataggagat gaaattctat caaaatacga tttcttgttt ctatatgata tacaagaaca
    16321 aataaattca ttagaaaaat taaaaaatat agattttgag tacttagttt tagggcatgg
    16381 caagcaagtt atatcgaaag aagattctta taaattaata gaaaaacatt tgaatgcttt
    16441 aaaaaaatat ttatatcaaa taagagagtt gcttgtagaa ccgaaatctt tagaaatttt
    16501 attaaaaaac attataaata acaataattt aagcaataat tataaagagt atcatttttt
    16561 taaatcttcg ttagtatctg tcataagtta cttagttgat ttagaagaaa tagggtatat
    16621 attggaaaat ggagaattgc tttactatac aaaaacaaaa taaattatga taaaataaag
    16681 aaggtcataa aaaacaaata aagggtgagc aatatggaat acgaaaaatg gaatgagata
    16741 ctagcacctt atgaacatgc agtagaggaa ttgaaaataa aatttaaaaa tataagaaaa
    16801 gaatatttag caaaaggtga atattctccc atagagtttg tcacaggtag aacaaaaaaa
    16861 atatcttcca taatatctaa gttaaaaagg ataaatgcaa aagatataga aactgaaatt
    16921 gatgatattg ctggtataag aataatgtgc cagtttgttg aggatatata tgctatagtt
    16981 gatttgataa aagtaagaaa tgatatgact ataataggag aaaaagatta tattacaaat
    17041 tacaaagata gtggttatag aagctatcat gttataataa aataccctat aaactccatt
    17101 gcgggttcaa aggaaattat atgtgaaata caaataagaa ctcttgcaat gaatttctgg
    17161 gctacaatag aacattcatt aaaatataaa tatgaccatt acatacctga gactcttgct
    17221 gaaaggctta gaagagcatc tgatgctgcc tttcttttag accaagaaat gagtgaaata
    17281 agagaggaca taatgaaagc tcaggctatg taccaaatga agtctatagc tattagagat
    17341 actttaaata gaatacaaga gttatatgac ttgggtgata cacataaagc tatgcagtac
    17401 caaagaaaac ttgatagaac tgaaacagat aaaaatataa ctgaaatttt ggaattaaaa
    17461 caagaaatag atatattatt agagcaatat aaggatacta aggtagatga taaagtagtt
    17521 cagtaaaaac tacttgtaga aaatttgcta aatattaatg gggctgcctc ttaatggaaa
    17581 ataatttcta aaaagagaca gcctatttag tgtaattaag aaaggaatga tattatgagt
    17641 ttgtatgcta taggtgattt acatttttca acatcagtca ataagccaat gaatatcttt
    17701 ggaagtaatt gggatggtca tgaaaagaaa attatagata attggaaaga agttgttaag
    17761 gaagaagata tggtcttagt actaggagat acatcttggg gaattaattt atccgaagct
    17821 aaaaaagatt tagatataat aagcaaactt ccaggtcaga aaatacttat aaagggaaat
    17881 catgattact ggtggacaac cgttacaagt ttaaataagc tatatgaaga catgcgtttt
    17941 attcagacaa atttttatga atataaagat tatgcaattt gtggaggtag gggctggata
    18001 tgtcctaatg atgttaaatt tgatgagact gatgaaaaag tttataaaag agaagaacat
    18061 agacttagac tatcattaga atctgctcgt aaaagtggtc actcaaaaat aattgtgatt
    18121 actcattatc ctcctacgaa tgataaattg gaagaatctt tatttacaaa gttatttgaa
    18181 gaatataatg ttgaaaaggt gatttatggt cacttgcatg gtaaagaatc ttttaaaatg
    18241 ggattgaagg ggattcgaaa tggagtagaa tatacattag catcttgtga ctatacagaa
    18301 tttaatttaa taaaagttca cgattaaaaa acataaattg gtaaatacta taagatacat
    18361 gattaaaaat aaatatttga gggatttcgg ttttgagaaa ggagacacga cttatgaggt
    18421 ggatattagt ttttatattt ttatatctta ttcctttagt agtgatattc agaaattaca
    18481 agaattttaa gaggtcttgt atctacagta gtatttatgt ggtattagct acaacaataa
    18541 tgataagtaa tatttatatg agtggactta ataaaataaa agagtctatg tattatcaca
    18601 attatatttc tgatgagaca tatgatgaaa agtatgtatc aaattttaag gaaaaagaaa
    18661 gtactgattt aaaacaggat aataaaaaga atgaagaaat atcaaataat aaaaatttaa
    18721 acaacaaaca agatagaaat atacaaaata aagaaagtaa tgaagaagga agtaatgata
    18781 taaaagaaaa gaataattct gatagtaatg tttcagtgaa taataaatca gaaaaacaag
    18841 aagataatac aaaagaaaat aataaatata caagtaaaga aaaaaatagt gaagatataa
    18901 aaaaaataga tatggaaagt ataggaattt ttaagaaaga aatttataat atagaaagag
    18961 cagctttagt tccaatgaga gaatgtatac cttacactaa ggatatagca aaaaatataa
    19021 ctaaattatc tagtataaaa aaagatgtta caaatgccag aaacaagtgt aaagaagttg
    19081 taaaagaata tgaaaatatg cagattccag aactttctaa ggagaagtat acagaaacat
    19141 tgagtaatgc tagagatgac gtaaagaaag catatgagct tagagaaaaa tctatggaaa
    19201 gtgctttgaa attaataaat actaaaagcc ctaaatatat agggaagata acagaatatc
    19261 ttgatttatc agataagcag atagaaagtt ttaaaaatag attgaacgat ttaaaagaag
    19321 aaataaataa ttcagaaaca aataatatta attaataaga tttaaagata taagttaaaa
    19381 gggagattat gatgaataaa cagagtatag gaaaactaaa tatgtttttt atggggatta
    19441 ctcaaagaaa ccttgataat agggattttt ttattgagtt agaggtgatt tttaaatctg
    19501 gaagaaaaga gtttaaagga attgcaatag aggaagataa taaatataaa tttaatttta
    19561 aaggaaaatc agatttatat acgtttaatg agttgcttaa gaatatttct aaagaggcag
    19621 aaaattatga tggattagta tttaaatatg ttgagcgagg aactattgtt gtaattgaag
    19681 gagacaataa gaaggtaaat gtaaaatatt tagataataa ggaagaagta cctaaaatag
    19741 atgaatttac ggcatctcaa ataaaaaaca gagattatta tgtaaaagta ggacaagcaa
    19801 atgcactttt aaaagaaatt ggagtactta caaaagatgg taagataaaa aatgataaaa
    19861 taagaaaata caatcagata gaccattttg ttgaattgat agatagtatt ttaaaagaaa
    19921 taaaggataa agactgtata actatcttgg attgtgcttg tggaaaatct tacttaagct
    19981 ttgtcttaaa tttttatata aaagaagtat tgaagaaaaa ctgttatttt ataggaatag
    20041 attattcaga tgtagttata gaagcatcaa aaaatatggc taaaaacttg ggttataaaa
    20101 atatgtcatt tataaaagag gatttgacta attatacacc taatagggat gttgaccttg
    20161 taataagtct acatgcttgt gatacagcta cagatatggc aataggtcta ggaattagag
    20221 caaaatctga agctatagta gtagtaccat gttgtcataa ggagttatta gggcaatata
    20281 gatatgaagc tatggaacca atattaaaac atggtgtatt taaagctaga tttgcagatt
    20341 taataactga tggtttaaga acattgttat tggagggcaa tggatatgat acttctgtag
    20401 tagaatacat atctccactt gatacaccaa aaaatttaat gataagagcg ataaaaacta
    20461 aaacgaataa tgacaaagca ttaaaagaat ataaagaact gaaatcacaa tttggagtgg
    20521 aaccaacctt agaaaaactt atatattagt taaaaatgta aaatttacga aaagcttgct
    20581 taaatatgca agctttttgg tattatatag gtatagtcat atccagagtt ttgatttata
    20641 attaaagctg tgctataata tttgattaat caaatcaata aatgttacat ggaggtaaaa
    20701 atgaacaaat tgaatatatg tattgatata gatggaacaa taacaagccc ttatcatttt
    20761 cttccatacc taaatgatat ctttaataaa aatataactg aagcagagtg tataacacat
    20821 aattgggaag aattgtatgg ttcgggaata agagatgtgt atgcggagtt taataataag
    20881 tatatacact catatgatga agcaaagatt gtagagggtg ctacagaggt aattagcaca
    20941 ttatctaaag gacataattt atcttttgta acggctagac atgagtgttt aactgatgta
    21001 actaaaaatt ggctatcgag acaaggattt tcagatatag atgtttactt attaggaagt
    21061 gattataaag ttgaaaaggc aagagaactg agttgtgaca tatttataga agataatcct
    21121 ttaaactcag tgcaactagc tgatgatggt gttaaagtaa tacttctgga tactaactat
    21181 aataaagatg ttaaacatca aaatatagtt agagttagta attggataga tattgaaaag
    21241 attataaaac aaggtattta aagatttgta gattgaaagg gggagcatat gatagattta
    21301 aaaattgaaa atagaaaagg aaaaatccat gtaaagagca gtgaaataaa agacatttta
    21361 gaattacgtc cagacttcga atatgtacaa gatatatcaa atactataaa tcaagaaaat
    21421 atattggtat ttgattgcca gttgacagga aattcttttg atatggatga tttagatata
    21481 gaagaaatat tggaagagtt aggtgaagaa atagatgagt cttatttcag tgttttattt
    21541 gaagatgtaa gagcgtattt aaaagatgct actgatgaga tagaagcaga cttacaggat
    21601 aattacttat ttgataattt gagatgttat tttgatatat acaatataaa tcaagaattt
    21661 acagatttta agtttgtatt tttagtttct tttaaagata taaaaatttc ttcattagca
    21721 aacctagcta agattgtttc taagagacag cttataggtg catctaagtt ttatagttaa
    21781 aatgaaatat tattataata aaagctagta accttgtaaa aatataggtt actagttttt
    21841 tatttattca ttttcacaaa atttcagaaa actatttaca ttctaaaaaa agtaatgtat
    21901 attaaacata atgtacatta taatggataa aaagtttcta ttaaattact aagtttattt
    21961 aataatcaaa ataatttatc ttatattagt atgtattagg gggtatttat gcaaagttta
    22021 aatgaaataa ttgctgaaaa tttaaaaaag ataaggaaag aaaaacatct tagtttagac
    22081 aagattgcac aacttagtgg tgtaagtaag agtatgctat ctcaaattga aaagtgtgag
    22141 gtaaatccta ctatatctac tttgaagaaa ataactaatg ggctaaaaat ttcatttaca
    22201 tcattgatgg agaggcagga aagtgacata gaattgattc aaaaaagtga tatagaccat
    22261 tttattgaag ataatggaaa gtatataagt tatccaatat ttccatttga ctccaaaaga
    22321 agatttgaga tatttatgat tgaaatagaa gaaggtggaa atttagattc aaatgctgaa
    22381 cttccaggaa cacaagagtt tataactgtt tttagtggcg aagtaaccat taaaataaat
    22441 ggagaagatt acattgtatc tagtggaaat tctataagat ttaaagctga tgtatcacat
    22501 atatatagaa actcaggcaa aggtatagct aaaatgagta tggtagtata ttatatataa
    22561 ataatttaat ggaattagag tgatatttag atgattttag gtatgaagat atattatatt
    22621 taagttatag gagtgtgatt tatggatgaa taataaaaaa gcagtttttt tcttttgcag
    22681 tgatttaaag aaagacccag tagccagtaa tgtgataaaa tgttgtgaag aaataatgga
    22741 tttaaatcaa actgatattg ttattgatga taattttgta ttagagttta aggataaaaa
    22801 caataactta tttcattttg taaaaacaaa agatgtaata agtcataatt ataaatatta
    22861 tcttccaatt ttaaatagac attttagaga ttatgatttt gcaggagttg taaattggca
    22921 tgagggagaa aatgctccag aacatatcct aacagctcat acaattggag atgttccaac
    22981 aggagaattt ggaaattcaa atccaagata ctttaaaaat ctaatcaatg ctattgagga
    23041 tataaaaaat gaaaatctac ttgatgaatt tactacatta acagaagcaa ctcactggtc
    23101 tggaacaaca tatggtgaag aatctaaatt aattacagaa tattctgtac ctatgttgga
    23161 tattgagata ggaagtagta gtgatagttt taataatagt atagcaatac aggtattggc
    23221 aaaatcatta attagagtat ttgattgtga tgaacctttg aagactttgt tatgtgttgg
    23281 aggggtacat tttgagaaat ctttctcaga tattataaaa aataaagaat ataacatatc
    23341 aattggacat gtattgccaa atcaatggat tgtaagtgga atgtatgatg atgaatctgg
    23401 atttgagaaa ctagaaaagt gtataaatag cattgaagga ggaattgatt gtatagtttt
    23461 tcatgataaa ttaaaaggta cctataaaga gcaatgccgt aaactaggtg aaaaacttaa
    23521 tgtacctgta tttaaacata aaatattaaa aaatccaaaa gacttaccta tatggtagtc
    23581 ttatacagtt aatataacaa taagtcggat taatttttaa gcgaggtcaa aaatgatatt
    23641 atcttatttt gcattattaa ttagagtagt gctattagct tttgaaagag tagttgtaag
    23701 attattaggt gatgaagaag gagatattta taaaaatata gcttcatcat ttctattttt
    23761 ctttataggg ggagtatgtt tacttccatt tagcataatg gatagagtta gtgattggtc
    23821 gtttttaata ccatgttata taagtagttt agtctattca attggttcag tagcgtatgt
    23881 aacgtcttta gcaactgggg aagtttcact tgttactcca ataaatagtt taaattcatt
    23941 gtttttactg cttttatcag ttatattttt aggtgaaggt ttaagtttag caaaagttgc
    24001 aggaatcata attatgattg gtggagtgtt tatattaaaa aaagtaagtt cttttttaaa
    24061 gaatgtctct gtaattttta acaatttacc atgtagatta atgtttttat acatattttt
    24121 gcagtcagta ggaagagtat tggataaggc tttttttgta caagtatctc ctatattata
    24181 ttcaactata ctgtattttt tcgtaggttt aaatttattt atctttttag catttaaaaa
    24241 gaaacaaaaa ataatttgtg agatttttac taataaaaag gggctttcaa ttttaagtgg
    24301 tgctattaat ggatattctt atttagcatt attaattgct ttaaataatc tagaacttag
    24361 tatagcagag ccattttctc aagtatctat gataataact ttaatacttg ctcattttat
    24421 attcaaagaa aatataaaag aaaaaatacc aggttcaatt ttgatactaa ttggtgggtg
    24481 gctactgtta ctttaattga cataaaaaat gaattataaa ggtgctatta gttagtatca
    24541 gaaaatttaa aaaagtattg acaattcttt taggcaaatg ttaatataat aatacaaaat
    24601 agtataaata gaagacatgt tactggtaat gcaggcagga tcgaatatat tgctttgatt
    24661 tttcaaggtg tttatatagg ttctgccttt tttgcatctt gaatggccat tcataaaaaa
    24721 ttatggagaa ggagattaca agatgaatta taaaaaaact gccattgaca ttattgaaca
    24781 agttggtggt tcaaaaaata ttttagcttt agaacactgt tcaactagac tacgctttaa
    24841 gttgattgat agaagtaaag tcaatcaaga tgctttaaaa aaagtacaag gtgttatgag
    24901 tatagttaat ggggcacaat ttcaagttgt cattggaaat agtgttgttg atgtgtatga
    24961 tgaaatttta aaagtttgcc ctttagatac aaatgatagt actgtaaaaa ctgaagaaaa
    25021 aataaactta ggaacaagat ttatagaatt tattattaat atttttcaac cactgatttt
    25081 tgctattgca ggagctggta ttttaaaatc actgttgatg ttattagcaa tgtttaatat
    25141 tttaaaatca gattctacta tatatacatt attagttgct atttcagatg caacattcta
    25201 tttcctacct cttatggtag ctgtaactac tgccaatgta ttgaaatgta atcgtttagt
    25261 agctattgcc gctgtaggat acttattgct tccagcaaca acaactgctt taagtgaagg
    25321 tgttgaacta tttggattta caattccaaa cattgcctat aatgcacagg ttttcccggc
    25381 tatcttatgt gtgtctttct tggcaacaat ggagaaaatt tttaacaagt attcaccaaa
    25441 accaattcga actttctttg ttcctatgat gtctttggca attactgttc caattactct
    25501 aactatttta ggtcctttag gatataacgt tggtacaatt tttacaacca ttattttatt
    25561 cttatataac aaaatgggat tcttagttgt aggattactt gctgcttttc ttccatttat
    25621 ggttgcaaca ggaatgcaca aagcactaat accttattca attaatacaa ttggaaagtt
    25681 gggttatgaa gctttatata tgacatcttc attagcccac aatatttctg aaagtggtgc
    25741 ttgttttgca gtagccttgc gaacaaaaga tgaaacatta aaacaaacag cattatctgc
    25801 aggaatttct gccttgatgg gaattacaga gccagcttta tatggtatta ccttacaaca
    25861 taaaagagca atgttaggtg ttgtattctc aagtgcaatt tctggtacct ttttaggatt
    25921 atttgcagta aaaggatttg taattgttgg atcagggctt gccagtatga gtatgtttat
    25981 tgacccaaac aatggaaaca atttgatttt tgcgagtatt ggttttgtta ttgcaatatt
    26041 aggctcgttt attattacac tgattatttg gaaggaagat acaaatacag tagagtcatc
    26101 aattggagat gttgttgaag aagagctaga agaagaaaaa gaagtaaata tcttagtaac
    26161 tccagtagaa ggaaaagtaa tagatttaag taaggttaat gatgaactat ttgcaagtaa
    26221 gactttggga gatggagttg caattatacc aaccaatgga aatttatacg caccttgtga
    26281 ttcagaagtt gtgatgttat ttgaaacaaa acatgcaatt ggtttaagaa caaaaaatgg
    26341 ggctgagatt ttgattcata ttggaataaa tactgtaagt atgaatggag atggatttaa
    26401 aacatttgtt aagacaggcg ataatgtaaa agaaggagat ttattaattc aatttgattt
    26461 agacaaaatc tcacatgcta atttagattc aactgttatg attgtaaaca ataatggagc
    26521 agattatgct tataaagtgt taaaccagtc ttatggcaat gtaaaaaaag gaagtatctt
    26581 gtttgatgta aagggaggaa tataaatatg agaaaatttc cagaaggatt tctttgggga
    26641 ggagctacag ctgctaatca atttgaaggt ggttggaatt taggtgggaa agggtggtct
    26701 gtatcagatg tagcaaaggc acactttgat gcagatgtga aagattataa atctaataat
    26761 gaaatcacaa caaaagatat agaagaagga ttagcacatc ctgaagatga agtaaattat
    26821 ccaaaacgtc atggtagtga tttttatcat cactataaag aagatattgc tctaatggca
    26881 gaaatgggat ttaagacata tcgaatgagt attgcttggt ctcgtatttt tccaaatgga
    26941 gatgataaag agccaaatga agaaggttta cagttttatg atgatgtatt tgatgagttg
    27001 ataagttatg gaattgaacc acttgttact atgtcacatt atgaaccacc attaaatatc
    27061 gttttaaact atgatggatg gtattcacga caagttatca atatgtttgt taggtatgta
    27121 gaaactatct gtgaaagata taaaaacaaa gtgaaatatt ggcttacatt taatgaagtt
    27181 gattctatga ttcgtcatcc gtacactact ggtggtttag ttcgagaccg ttttaaggat
    27241 aaaaactttg aggaagttat ttttcaagct atgcatcatc aatttgtagc tagtgccatt
    27301 gcaacaaaaa tttgccatga gattattcca aattcaaaag tagggtgtat gttaacaaaa
    27361 ttaacgtatt atccatatac atgtagacca gaagatgttt tggcaactca acaaaaaatg
    27421 agaagtattt atgcatatag tgatacgcag gtttttggtg agtatccagt atatttactg
    27481 tcgtatttta aaaataataa cattcaaata gtaaaagaag aacatgatga tgaaattatg
    27541 aaaaagtatc cagtagattt tatttcattc tcttactata tgtcttcttg tgaggcagct
    27601 gatacaactg gattagatat tacacctggg aatacgttat tagctgtaaa aaatccttat
    27661 ttagaaatga gtgaatgggg ttggcaaatt gattcaatag gtctacgtat atctttgatt
    27721 gaattatatg atagatacag aaagccttta tttatagttg aaaatggatt gggagctaaa
    27781 gatattttaa ctgaagataa aaaagttcat gaccaatata gaattaatta tttaaaagaa
    27841 cactttaaat gtatgttaga tgcgattatc gaagatggcg ttgaactttg gggatatact
    27901 tcatggggtt gtattgattt ggtcagtgaa tcaacgaaac aaatgtcaaa acgttatgga
    27961 tatatttatg tggatgctga tgactatgga aaggggacat ataatcgata taagaaagat
    28021 agtttttatt ggtataaaaa ggtaattgaa aataatagta tagattttaa atgaccaata
    28081 gaaggtgagc aaataaaatg aactacataa taaagaaagt tttgaactcg agcgttgttt
    28141 tagttcatga tgcaaagtat aatgaattta ttttgctagg gaaaggaatt ggatatggta
    28201 aaaaaacagg agaatatatt tgtggttcca atgataatca aatgtttgtc cctgttgaaa
    28261 atacaaaatc aaaacaattt ttagaattaa tggatgatat tcctgtcgac attttaaaga
    28321 taacacagga aataatagta gaggcagaaa agttattgaa ctcatctttt aataaaaact
    28381 tatacttaat attagcagac cattttaact ttgcaattga aaggatgcgt aaaggaataa
    28441 aaattacaaa tcgagtattt tgggaaataa aaaattatta tccaaatgag tttaaggttg
    28501 gaatggttgc aatttatatg gtagaggaga gattgggcat tcaattacca gaggaagaag
    28561 cagctaacat tgctttccat tttgcaaatg ctatggcttc agatggaaat tcatatgata
    28621 cgattaagta tgccaaagta attggagaaa taattaatat ttttgttttt tctttgaata
    28681 gaaccttaga taaaaagagt atgcattata tgagatttat aacacatata aaattttttg
    28741 tggaaagatt tttttcagat agtatgttaa gcagtggtga tgatttatta tttaatcaaa
    28801 tgaaaaggtc aaatccaaaa gaaatgttaa ttgcattaaa agttagagat tttttagaaa
    28861 agaaatatgg aaaaaagtta acaaatgagg aaatagcttt cttggttgta catatcgcac
    28921 gagttaagca atagagtgaa ttactaatat tatcttatac gtctacatgg tagaaaattg
    28981 aaagaaggta aagtatataa agaaactgag acatattatt ttgttgttca ttatgtttat
    29041 tttatgagtg tatcctgtta aaaatttaag ttaatat
//
LOCUS       DAMJVK010000046        28680 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.046, whole genome shotgun sequence.
ACCESSION   DAMJVK010000046 DAMJVK010000000
VERSION     DAMJVK010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28680
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.046"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(234..2513)
                     /locus_tag="PC414_002089"
     CDS             complement(234..2513)
                     /locus_tag="PC414_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2464484.1"
                     /translation="MLSDAKNNRYGLSIIAVISILVITISCLIYGSRLNKTLGEETNQ
                     YLSEIANQSVNVLKKQINGDIKLLESISIFIEGEESFEVDNILSILKRQAINSSFKRM
                     GVILPNGTAYTTDGYIEDFSKRDYFLKSMQGEVVITGKLKDVIEDDKNTNSNINVYSV
                     PIYKDNEVKGVIFATHSTKLYEKILSVPTFNGKGYSYITRSNGDIVLNPSSKNANSTK
                     ENLFSYIGRSDSISKSSINNMKSNIKNFKSGSLSYSMFGKGYYLSYAPIGINDWYLFS
                     EVPKTAISEKSYAIIKLTLSACVVLIIIFTALTIYILFIHKKSKEKLEEFAFKDNVTG
                     IGNSNKFNLEGGKFLSSHEKKNLVLIYFDIDKFKLVNDRFGYEEGDRVLKEIAEIIKN
                     IFKEQSVFSRISNDNFAIIFEKKNNRESIIEICELIRKKLSMIKTSLGVELNLIPSIG
                     VYFVEEGETNISTCLDKAMIAKTTVKRKYLKVYEIYAENLKETLIEERDIEQEMHDAL
                     KNEQFKVYLQPKVDLSTTKIVGAEALVRWQHPKKGLISPGVFIPIFEKNGFITELDMF
                     VFTQVCKNFKRWENENFPTFPISINLSRVHLENPGFISELERITKEYEIEPNLIEIEL
                     TESAIFDNTKILFKIMQTLKSVGFKISMDDFGSGYSSLNMLKDMPIDVLKLDRQFFIT
                     VGNAKKSQIVVSSIVQMAKQLDIKVVSEGVETVEQAEFLRFIGCDMAQGFLFARPMPI
                     EEYEKLILDRYSLNRKETN"
     gene            complement(2784..4238)
                     /locus_tag="PC414_002090"
     CDS             complement(2784..4238)
                     /locus_tag="PC414_002090"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HDF2464485.1"
                     /translation="MKLNDIIQGLDIINVKGELNIDINNVQYDSRKVTKGTLFICIKG
                     FVSDGHKYIKDAIEKGASAFLVEEDVAIKGCTFIKVKDTRKDMAKVADNFYNHPSQKF
                     NVIGVTGTNGKTSITTILNEILTLNKNKVGLIGTIKIFDGEKDIVSNSTTPESIDLQY
                     HFNNMLDNGCDYCAMEVSSHSLALNRVDETDFKLGIFTNLTPDHLDFHKDLEDYRKAK
                     EKLFFKTTMANIINIDDEGGKKIYENIKGINVPCYTYGVDTKADFMARDIKSDSDGVS
                     YRLITPSYEEVIFIPVPGMFTVYNTLAVIAACYVLGIPKPIYKEGLRLSNGVSGRFET
                     VPNDKGISVIVDYAHTPDALENVLKTTQQFAEGKIISVFGCGGDRDTEKRPLMGAIGQ
                     KYSDLCIITSDNPRTEEPEAIIKDILEGIDKKKENYHVVVDREQAIAEAISMAKKDDV
                     VIITGKGHETYQIIGKVKHHFDDKEVANECLSKM"
     gene            4402..5271
                     /locus_tag="PC414_002091"
     CDS             4402..5271
                     /locus_tag="PC414_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2464486.1"
                     /translation="MDNKIGYLNDNFKIFNIRDKKDIQFEYHNHDFNKIIIFINGNVT
                     YFVDGIPYKLKPWDILFISNNEIHKPVIDSNVFYERIAIWISPEFMKKNSDASSDLEK
                     CFKIATENKCNRLRLNMHDIESIKSFIEQINISESNTEFGNEILRNTLFIQLMIFLNR
                     IFLKNENMESTEDVFYDETIKKVLNYINSNIDDKLSIDSISSEFFISKYYLMRKFKSH
                     TGTSIHNYIVQKRLILAKSLITNGHLMSEVCNKCGFNDYSSFVRAFKKNYGVSPKNYI
                     NNKNPLDITLLDE"
     gene            complement(5484..7556)
                     /locus_tag="PC414_002092"
     CDS             complement(5484..7556)
                     /locus_tag="PC414_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2464487.1"
                     /translation="MKKVFGVLQKVGKSLMLPVALLPAAGILLGVSNALAGDALLAHF
                     PALANPTFQLIISIMESSGQIIFNNLPLIFAVGVAVGLTEGEGVAALAAIVGFLILNT
                     SMGVMAGVTEDMVDNSMYANIVGIPTIQTGVFGGIIIGIVAAYLYKKFYKIELPQYLG
                     FFAGKRFVPIVTSLVAIAIGIILSFIWPPIQEGLLSFSKNVIEANQTLAAFMEGSLER
                     LLIPFGLHHVFHNPFWYQFGEYVNKAGELVMGDQKIFFAQLKDGVNFTAGTFMTGAFP
                     FMMFGLPAAALAMVHEAKPEKKKFVAGIMASAALTSFLTGITEPIEFAFLFVAPVLFA
                     IHCLFSGLSYAIMQLLGVKIGFTFSGGLIDFLLFGVLPNRTPWYLAILVGIGFAVLYY
                     SVFRFMIRKFNLKTPGREDESETNAEVIEMSKNELASEILIALGNKDNLKSLDACITR
                     LRLVVNDINKVDKEKLKSLGAAGVMVIGNNVQAIFGPKSDSIKSDIKDIIEGKTKVES
                     TVTKNVDKNKVKKNMEVANMENTLVEKIDSLTNGELLDIEEVPDSVFSSKLMGDGFAI
                     KSSDGLIYSPVDGTIGVIFPTKHAIIIKSQKSGVEILIHLGIETVNLEGNGFEVFVNV
                     GDEVKAGEKLVKMDLEYIEKNGLSTISPVVFTNLEQNQKLSIKKGVTTAKEENRVSIL
                     RDDIEKIG"
     gene            complement(7859..8701)
                     /locus_tag="PC414_002093"
     CDS             complement(7859..8701)
                     /locus_tag="PC414_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2464488.1"
                     /translation="MIIQQIYNNNVVLVLDENIKKELILTGCGIGFQKKKGQEIDKSK
                     IERTFVIQDESFLDKISKLASQVDEKFFEVSTEIIAYAEENLNTKLYEYIYVALTDHI
                     AFAIKRYHENITIKNDLLHEIKRIHKKEYEIGKWAVDYINKEFDVKFPVDEAGFIAMH
                     IVNSNYKGSSKESLLITKIVKDILNIIRYYYRVEFKEDDINYDRLLTHLKFFAKRLVK
                     KEKINDTNNEIIDIIKVKYEKDYDCAYKIKTHVEKNYDYYVSQDELLYLTLHIKRVIS
                     VLNL"
     gene            complement(8962..11004)
                     /locus_tag="PC414_002094"
     CDS             complement(8962..11004)
                     /locus_tag="PC414_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HDF2464489.1"
                     /translation="MSILVTLLGLLVCTTISTVFSKKWSNIPLAIYQIVLGIILSILP
                     FKFSFSFNPEIFVICIIAPLLFSEGQNVSRKELLELRKPILLLAFGLVLITVFAGGIF
                     IHFLIPGMPLSVSFALAAVISPTDLVAVKSITQGLNFPKNMMSILEGESLLNDAAGVV
                     AFKVAVLATVTGVFSIEEAGIQFMITAFGGIIVGSILGYIIIKIRLSLHKWNLEEIPM
                     VIVIQIMTPLFVYFVAEEIGVSGILAVVMAGIAHGIEKEHLQNTTTKLRIISDNTWYV
                     LEYVLNGFVFTLLGFLLPSIYTGLSSKNENMALELTFISTLIVLILFVIRFIWVYLWH
                     NSFIKPKKNPLNNFFVGFLGFKDEEVVKESISKCKYSLIVATCGVHGTFTLATALSIP
                     FYLADKDVFPMRDTVLFISSEVILISLVLATVLLPRLLKNNVQESEPLLSDKEAYKLI
                     LKEAILKLSNEKAQEVQPVIHDLNEQLVDSEKGILNTPDNKLISMMIAEADQQGLLAV
                     LKLLEDGKISDRAFKLYRFYITKSRKSVQKSIFKIVKLKISMWIINRKVKKEVKEKIT
                     KIVSENKEIIKEFHHAYALSTKTAISFINKNTDEDNRHEALIAIRFYNRYLNWITRKI
                     QNDDKFEARVEYYRVMAIQSQRDSVQQLVETKQISREVAKELLENILYDEMMMIES"
     gene            complement(11156..12127)
                     /locus_tag="PC414_002095"
     CDS             complement(11156..12127)
                     /locus_tag="PC414_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HDF2464490.1"
                     /translation="MKKEESLLKVEGLKKYFEVKSGLFDKEIKCVKAVDGISFDLKKG
                     ETLAIVGESGCGKSTAGRTILRLIEKTEGKVEFNGVDIYKLEKEELRKLRPKMQMIFQ
                     DPYSSLNPRLNVEQIISEAVIEHNLVPKNEIKDNILNVVESCGLSKYHLNRKPGEFSG
                     GQRQRIGIARALALNPEFIVCDEPVSALDVSIQSQIINLLMDLQDKYKLSYLFISHDL
                     SVVNHIAHRVCVMYLGSIIEIATKGELYLHSQHPYTKALMSAVPIQDPTIKKNRIILK
                     GDIPSPTNPPSGCKFHTRCPYCKEICKKEIPVLKEISRNHFVACHLV"
     gene            complement(12128..13105)
                     /locus_tag="PC414_002096"
     CDS             complement(12128..13105)
                     /locus_tag="PC414_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2464491.1"
                     /translation="MSKALIEVKDLKVYFHTDKGIVKSVNEVSFNINEGETIGIVGES
                     GCGKSVTAMSLMKLLPTSKIEGGEIIFRGKDILKMNEDELMGIRGNEISMIFQEPMTS
                     LNPAFTIGSQIIEGIMIHQDLSKEEAKKKVIDMIKLVEIPRAEEIYNSYPHELSGGMR
                     QRIMIAMALSCNPKLLIADEPTTALDVTIQAQILDIMKNIKEKLNTSIMMITHDLGVV
                     AEMCDKVLVMYAGKIIEVAEVVELFKNPKHPYTIGLLKSKPALGKNKDKRLYSIPGQV
                     PNPIGMPDSCYFSDRCEKVCDKCRTQIPPLIELNSGHSIACWLYKKEEI"
     gene            13324..14883
                     /locus_tag="PC414_002097"
     CDS             13324..14883
                     /locus_tag="PC414_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2464492.1"
                     /translation="MKFKKLASLILVSSLMLTFTACASNDKDKSSGSKSGGTVVIPMA
                     SDPDIINGAFANVKEDSLASNIVYAPLYTYEKGNLVNYLAEKVDFKDSKELTIKLKSN
                     LKWHDGKPITAEDVLFTFNTVLDEKQNSPSRQYLLVGEKPVKVEKIDDLTVKITLPTA
                     SESFLYGISKISPIPKHVFEGESNIAKSEKNNNPVGSGAFKFKEWKKGESIVFEKNAD
                     YFGGESKADSIALKIIPNEASQEAALNNGEISLMKTSAEGYEKAKSNSNLQTYTYSEE
                     RLNYIVFNQNISNMANKEVRQALSYALNRNEMIESAYGKEGSVPAKSILVPEADFYTE
                     EGVEGYDQDTNKAKDLLDKSGVKIDKLKIGYNTGRFGHKNYALVAQQELKKIGIEAEI
                     VPYESKAFFNILFSNSTECDMYVNGYAWGLEPNPYRGMFETGQYCNQTKYSNAEIDAL
                     WEKGFTELNKEKREEIYKQIQQDISKDAPIYTIDYEQNLMAAQKNLKGIKDAKPSPAI
                     LFEDWSKLYVE"
     gene            14989..15948
                     /locus_tag="PC414_002098"
     CDS             14989..15948
                     /locus_tag="PC414_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2464493.1"
                     /translation="MLKLILNRVKVLIPMLILISILSFGLLELAPGDPADAYINPLMS
                     SQDIENIRVNMGLDKPAYIRYLNWLKNTLSGNLGISYINHMPVTEQIMEKMGNTFILM
                     GTSLIFSILVAIPLGIFLAVNKNSITSKVSSVFNYIGVSIPSFWIGMILISIFSVKLN
                     IFPSGGMHTIGNDSIGDLAKHLVLPVITLGLYNTAIFTNYVEASVNEQLKKQYVITAR
                     AKGLSEKVILFKHVLKNSLTSLVTILGMSIQKLVTGAFVTEVVFSWPGMGRLMIDSIF
                     SRDYTVIMAITMLSALFLILGNLVADILYLLIDPKIKSSKGGF"
     gene            15951..16763
                     /locus_tag="PC414_002099"
     CDS             15951..16763
                     /locus_tag="PC414_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2464494.1"
                     /translation="MFTSILKKLKKDKSFAFACITLSIIIILIIASFILNIDSNAIDT
                     TSKLQEPSLKHIFGTDELGRDYFSRALYGGRISLSVGILSMLISVVFGTAVGVISGYI
                     GGKLDQFLMRLVDMLMSIPSFLVLIVLNTYLTPKVSTIILIIGFLSWMEVSRIVRGET
                     LKIKENEYCLAAKATGIKTRNILLRHIVLNLKETIVVAGSLNIANAILTESALSFLGL
                     GIQLPLASWGNMLQDAQRHIFDKLYLAVFPGLLIFLTVMSLTIISKKFSSNT"
     gene            complement(16979..17881)
                     /locus_tag="PC414_002100"
     CDS             complement(16979..17881)
                     /locus_tag="PC414_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2464495.1"
                     /translation="MDIKQLKYFVEIVKSGFNLSIASKTLHISQPALSQIIKNFEESE
                     DVYLFERYKGRLNGLTPAGERFYVNAEHIINEYKNMMEDLREDSVQFKGKVRIGIPPL
                     ILGIAFSDVVAQLVANNPDIEFDIVEKGAYDLNRMLILQELDYAVLLHPHKIDKSVVT
                     EHVLQEDELTAFLNINHPLAKKDKIDWNDLNEQLLAIFNPTFMIHHKLMQKFTDENVK
                     LKRYIMSGSWDFLLLSTTNSDFITILPSPVHDFFENSKIIEKPFNHPITWKVILCRPK
                     KERYSHVDQHVFKFIIDFFSKKNS"
     gene            18236..19414
                     /locus_tag="PC414_002101"
     CDS             18236..19414
                     /locus_tag="PC414_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HDF2464496.1"
                     /translation="MKDVVIVSAVRTPIGSFGGVFKNTSAVQLGTIAVKEAISRVRLN
                     LSEIDEVIIGNVLQTGLGQNVARQIAINAGIPNSVPSYTVNKLCGSGLKSVQLAAQSI
                     TSGENDVVIAGGTENMSQAPYIVPTARFGSKMGDITMVDSMLTDGLIDAFNQYHMGIT
                     AENIATKFEFTREMQDKLALESQNKAENAIKNNRFKEEIVPVDVLIRRGKIETIDKDE
                     YPKLGMTFEGLSKLKPAFKKDGTVTAGNASGINDGAAMLILMSQQKADELGIRPLAKI
                     KSYASAGVEPEVMGTGPIPATRKALKKAGLSINDIDLIEANEAFAAQALAVKNELQID
                     SSKLNVNGGAIALGHPIGASGARILVTLIYEMQKRKVETGLATLCIGGGQGISMVVSR
                     "
     gene            19423..20079
                     /locus_tag="PC414_002102"
     CDS             19423..20079
                     /locus_tag="PC414_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HDF2464497.1"
                     /translation="MNKIVSIDEALSHVKDGDTIMVGGFMANGSPENLIDYLCSKNIK
                     DLTLICNDTGFIDKGVGKMVVNKQFKKIIASHVGLNKETGRQMNNNETDVELVPQGTL
                     AEQIRAAGYGLGGILTPTGIGTLVEEGKQKIVVDSKEYLLEKPIYADVALLFASKVDK
                     AGNLVYKGSMNNFNNLMASAAKITIVEADEIVEIGDIDPNEVNTPGIFVNYIVEGGNL
                     "
     gene            20079..20744
                     /locus_tag="PC414_002103"
     CDS             20079..20744
                     /locus_tag="PC414_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HDF2464498.1"
                     /translation="MDKLEMQEYIANRVSKELKDGAVVNLGIGLPTKVANYIPDNVNV
                     ILQSENGFLGLGSAEDGNSSDETIVNAGGQPVTILPGGCFFDSATSFGIIRGGHVDIT
                     VLGALQVDKYGNIANYMIPGKMVPGMGGAMDLVTGAKKVIVAMEHTSKGSAKILNNCT
                     LPLTATNAVDLIVTEMGVMEVTSDGILLKEINPAFTLDDVISATEAPLILSDSLNGKI
                     SVV"
     gene            20849..21625
                     /locus_tag="PC414_002104"
     CDS             20849..21625
                     /locus_tag="PC414_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HDF2464499.1"
                     /translation="MVKDKVVFVTGAASGIGKQIAESFLKNGAKVMFSDINQEALNNT
                     ITELQKKGYDCMSVKCDVTKEEEINHAIDKTVEKYGRLDILINNAGLQHVSMIEDFPT
                     DKFEFMIKIMLTAPFIATKKAFPIMKKQGFGRIINMASINGVIGFAGKAAYNSAKHGL
                     IGLTKVAALEAADSGITVNALCPGYVDTPLVRGQFNDLSKTRNIPLENVLEEVLYPLV
                     PQKRLLDVQEIADYAMFLASDNAKGITGQACLLDGGYTAQ"
     gene            21687..23021
                     /locus_tag="PC414_002105"
     CDS             21687..23021
                     /locus_tag="PC414_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDF2464500.1"
                     /translation="MSIIAPIGIIIGIIAIIYFSVKEIHITIAAPIATLIVVILNKMD
                     VVTSMLGADKNNYMGALGNYIMSYFAIFLLGSILAKFMEESGATVSIADFILNKFGSN
                     HPYRVLVAIFIVSFILTYGGISLFVVMFAVIPLARTLFKKLDISWNLIQIPLWLGIAT
                     ITMTIIPGTPAIQNVIPIQYLNTSLTAATIPSIVGSIGCATFGLVYMKYALNKSIRKG
                     ETYATYTSELEEESLDRELPGFFASIFPLLALVIIAITGSTFGSEFMKKNVIYIALIV
                     AILLSAFLFHKFLSSKIQTLSIGASGSIGPIFATSSAVAFGAVIMAAPGFDVFSKLIM
                     SIPGSPLISLTVLTSTMSAITGSSSGALGIVMPNFADYYLSTGLNPELIHRVATIASN
                     ILTIVPQSGVFLTFLSLSKLTHKTGFKETFIVVAVGSLIAEIIVITLGLLMY"
     gene            23517..24449
                     /locus_tag="PC414_002106"
     CDS             23517..24449
                     /locus_tag="PC414_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein"
                     /protein_id="HDF2464501.1"
                     /translation="MMRRNIPTKLKTILALGVLTLATASSAVTASASSLENNVNISNK
                     SLVYSNTKYKFSAKPNCSVKPELKPDKDNSCKDKNHNDKNCNNTNKPEDNNNSDSTNK
                     PDNNKPESNKPEDNNNSGSTNKPNNNKPESNKPEDNNNSGSTDKPDNNKPESNKPEDN
                     NNNNSDSTNKPDDNNNSGSTSESFSAYQKEVVDLVNIERAKAGLNPLTLDSSISNVAT
                     KKSQDMIDNNYFSHNSPTYGSPFDMLKKFGISYKTAGENIAMGQKTPKEVVNAWMNSE
                     GHRKNIMNPNFSKIGVGVAQKSGGSIYWTQIFVG"
     gene            complement(24841..28380)
                     /gene="nifJ"
                     /locus_tag="PC414_002107"
     CDS             complement(24841..28380)
                     /gene="nifJ"
                     /locus_tag="PC414_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HDF2464502.1"
                     /translation="MAKFMKTLDGNTAAAHVAYAFTDVAAIYPITPSSTMAEVVDEWA
                     SQGRKNIFGQTVNVVEMQSEAGASGTFHGSLQGGALTSTFTASQGLLLMIPNMYKVAG
                     ELLPGVFHVSARALASQALSIFGDHQDVMAARQTGCVLLASGSVQEVADIAPVAHLAA
                     IEGRLPFIHFFDGFRTSHEIQKVELLENEDYASLLNFEAVQAFRDNALSPNHPVARGT
                     AQNPDIYFQTREASNKYYQNIVGIVEKYMEKMSNLTGRKHSLFDYYGAEDAKYVLIAM
                     GSVTETIEETIDYLNAKGEKYGLVKVHLYRPFSMKHFLDAMPSTVERICVLDRTKEPG
                     STGEPLYLDVRDVFYGKENAPMIIGGRYGLGSKDTTPSDIKTVFDNLVSEQPKNGFTV
                     GIVDDVTNTSLTPSEPIKIASKGTIRCKFWGLGSDGTVGANKQAIKIIGDHTEKYAQA
                     YFDYDSKKSGGITMSHLRFGDTPIRATYLIDEADYIACHKQSYVYQYDLLKGLKKGGT
                     FVLNTIWDQAGLEEHLPAHMKQYIAKNDIKFYTVNAVKLGQEIGLGNRINMIMQSAFF
                     KLAEIIPEEDAVKYLKDSIVKAYGKKGEKIVNMNYAAVDAGINALVKVEVPASWENAV
                     DADKGEVKEPEFIKNILRPMTAQEGNNLPVSTFNGIEDGTFPCGTAAYEKRGIAVDVP
                     EWIIDNCIQCNQCSFICPHSCIRPVLVTEEELANAPEGFEAKKALGKGLEGLKYRMQL
                     SPLDCTGCGNCADICPAKEKALVMKPIDTQLDTELDNWAFAVNPDEVAPKGDVMPVNT
                     VKGSQFRQPLMEFSGACAGCGETAYIKVVTQLYGDRMMIANATGCSSIWGASAPSIPY
                     TCNHEGKGPSWANSLFEDNAEYGFGMYTAVKQIRNKIVDAMTELVSMGICEDAKAVFT
                     EWLDSRNDGEASKVASAKVVELLEKPACDCTDEKAKELVKAIKDRKDYLVKRSQWILG
                     GDGWAYDIGYGGLDHVLASGEDVNVLVFDTEVYSNTGGQASKATPAAAMAKFAASGKK
                     SRKKDLGMMAMTYGNVYVAQVAMGADKNQFIKAVIEAEKHDGPSLIIAYAPCINHGLK
                     EGMGRTVANEAQAVACGYWHLYRFNPEVKAEGKNPFTLDSKEPTASFKDFILAQVRYS
                     AIAKQFPEEADGLFAKAEADAKERYEGYKKLAE"
BASE COUNT         9868 a         4564 c         3751 g        10497 t
ORIGIN      
        1 attactatta ttactatcta aagtattttg ctataatccc atatactctt acctcaaaaa
       61 cattcaaatg taaaaaatac tacatattaa taaactttgc ccaataattc ctaaaataat
      121 atcctaacaa tgatttatgt ttttataata gtatattttt acataaaaaa gtgaacattg
      181 attaacaaag ttcacttttt tataaatttt tatgttataa ttcacaattt tatttaattt
      241 gtttcttttc tatttaaact atatctatct aatattaatt tttcatattc ttctattggc
      301 attggtctag caaataaaaa tccttgggcc atatcacaac ctataaacct taaaaactct
      361 gcttgctcta cagtttcaac accttctgaa acaactttta tatcaagttg ctttgccatt
      421 tgaacaatac ttgaaactac aatttgactc ttttttgcat ttcccactgt tataaagaat
      481 tgcctatcta atttcaatac atcaattggc atatctttaa gcatatttaa agaagaataa
      541 ccagaaccaa aatcatccat agaaatttta aatccaacag atttcaaagt ttgcattatt
      601 ttaaataata tttttgtatt gtcaaatatt gcactttctg tcaattcaat ctcaattaaa
      661 tttggttcaa tttcatattc ttttgttatt ctttcaagct cagaaataaa gcctggattc
      721 tcaagatgta ctcttgataa gtttattgat ataggaaatg tggggaaatt ttcattttcc
      781 caacgtttaa aatttttaca aacttgggta aatacaaaca tatccaattc tgttataaaa
      841 ccatttttct caaaaatagg aataaataca cctggactta ttagaccttt tttgggatgt
      901 tgccaacgta caagtgcttc agcacctaca attttagtag ttgataaatc aaccttaggc
      961 tgtaaataaa ctttaaattg ttcatttttt aaagcatcat gcatttcttg ttcaatatca
     1021 cgttcttcta tgagagtttc ttttaaattt tcagcataaa tctcataaac ttttaaatat
     1081 tttcttttta ctgtagtttt tgcaatcata gctttgtcaa gacaggtaga aatatttgtt
     1141 tctccctctt caacaaaata tacacctatg gatggaatta aattgagttc tactcccaaa
     1201 cttgttttta tcattgacaa ttttttacgt ataagttcac atatttctat aattgactct
     1261 ctattatttt tcttttcaaa tattatagca aaattgtcat ttgaaattct tgaaaataca
     1321 gattgctctt taaatatatt cttaattatc tctgctattt cctttaatac tctatctcct
     1381 tcttcatacc caaacctatc atttactagc ttaaacttat ctatatcaaa ataaattaaa
     1441 actagattct ttttctcatg agaggataaa aattttcccc cttcaagatt aaatttattt
     1501 gaattgccaa tacctgtaac gttatcttta aaagcaaatt cttccaattt ttccttactc
     1561 tttttatgta taaatagtat ataaattgtt aatgccgtaa agattataat caagactaca
     1621 catgctgata atgtaagttt tataatagca tatgatttct cagatatagc agttttagga
     1681 acttcagaaa aaagatacca atcattgatt cctattgggg cataacttaa ataatatcct
     1741 tttccaaaca tactataact taatgaacct gatttaaagt tttttatatt tgacttcata
     1801 ttgttgatac tagattttga tattgaatca cttctgccaa tgtaagaaaa taaattttct
     1861 ttagtagagt tggcgttttt actgcttgga tttaaaacaa tatcaccatt acttctagtt
     1921 atataagaat atccttttcc attaaatgta ggtacagaaa gtattttttc atacaacttc
     1981 gtactatgtg tggcaaatat aactccttta acctcattat ctttataaat tggtacagaa
     2041 tatacattaa tattactatt ggtattttta tcatcttcta ttacatcttt taattttcct
     2101 gttataacca cttctccttg cattgatttt aagaaataat ctcttttaga gaagtcttct
     2161 atataaccat ctgtagtgta tgctgtacca tttggtaaaa taactcccat tcttttaaat
     2221 gaactattaa tagcttgtct ttttagtata gaaaggatat tatctacttc aaaagactct
     2281 tctccttcaa taaatattga aattgactct agtaacttta tatctccatt aatttgtttt
     2341 tttaacacat tgacgctttg atttgctatt tctgaaagat attgattcgt ttcttcacct
     2401 aatgtcttat tcaaccttga accataaatc aaacaggaaa ttgtaattac taagatactt
     2461 atgacagcaa ttatggacaa accataccta ttattttttg catcacttaa cattatgctt
     2521 cctgcctttc tcgaatctat gtatgtataa tcattatctt atgacaatta ttgtaaacat
     2581 tttataataa tacttatttt tccatattta ctgtaattat acaaaattat aaactttatt
     2641 acaacaataa attaattaat atagatatta gtgtttacat tattgcatta tctattttac
     2701 aattatttac atataaaaaa atgtaatatc aaagtgaagc ttgatattac atttttgttc
     2761 ttgtattttg tttaattttt atgctacatc ttacttaaac actcatttgc aacttcttta
     2821 tcatcaaaat gatgcttaac tttacctata atttgataag tttcatgtcc cttacctgtt
     2881 ataattacaa catcatcctt ttttgccata cttatagctt ctgctatagc ttgttctcta
     2941 tcaacaacaa catggtaatt ttcttttttc ttgtcaattc cttcaagtat atcttttatt
     3001 attgcctctg gttcttcagt tcttggatta tcagaagtta taatacataa atcagaatat
     3061 ttttgtccaa ttgctcccat caaaggtctc ttttcagtat ctctatctcc accacaccca
     3121 aacacagaaa ttatttttcc ttctgcaaat tgttgagttg tctttagtac attttcaagt
     3181 gcgtcaggag tatgagcata gtctacaatt acgcttatac ctttatcatt tggaactgtt
     3241 tcaaaccttc ctgatactcc attagaaagt ctaaggcctt ctttatatat tggtttaggt
     3301 atgccaagta catagcaagc agctataact gctaaagtat tatatactgt aaacatccct
     3361 ggaactggta taaatattac ttcttcatat gaaggtgtta ttaatctata agatactcca
     3421 tccgaatctg attttatatc tctagccata aaatcagcct tagtatcaac accataagtg
     3481 taacaaggta catttatacc ttttatattt tcatatatct tttttccacc ttcatcatct
     3541 atgtttatta tatttgccat agtagtctta aaaaataatt tttcttttgc ttttctataa
     3601 tcctctaaat ctttatgaaa atctaaatgg tctggtgtta aatttgtaaa tatgcctagt
     3661 ttaaagtctg tttcatcaac tctatttaat gccaatgaat gtgatgaaac ttccattgca
     3721 cagtagtcac atccattatc caacatatta ttaaaatgat attgtaaatc tatactttct
     3781 ggagttgttg aattggaaac aatgtctttt tctccatcaa aaatttttat agttcctata
     3841 agtccaactt tattcttatt taaagttaat atctcattta gtatagtagt tatactagtt
     3901 tttccattag tacctgtaac tcctataacg ttaaattttt gagatggatg attgtaaaaa
     3961 ttatcagcta cttttgccat atcttttctt gtatctttta cctttataaa tgtacatcct
     4021 tttattgcta catcttcttc aacaagaaat gcacttgctc ccttttctat ggcatctttt
     4081 atatacttat gaccatctga cacaaatcct tttatacata taaataatgt accttttgta
     4141 acctttcttg aatcatattg tacattatta atatctatat ttaattcacc cttgacattg
     4201 attatatcca acccctgtat aatatcattt aatttcataa atgctttcct ccctttaaac
     4261 tctttctgct aaaattacac tacatataat attatattaa gaatttttta ctatgtgaat
     4321 ggttaaaaat gcttgaaaca ttgcaaaaaa tgctctataa ttttctataa tataactgag
     4381 gaaaggtctt ctataaaaac tatggataat aaaatcgggt acttaaacga taactttaaa
     4441 atatttaata ttagagataa aaaagatata caatttgaat accataatca tgattttaat
     4501 aagataatta tatttataaa tggaaatgta acttattttg ttgatggtat tccctataaa
     4561 ctaaagcctt gggatatact ctttatcagc aataatgaaa tccataaacc agttatagat
     4621 tcaaatgtct tttatgagag aattgcaata tggattagcc cagaatttat gaaaaaaaat
     4681 agtgatgcta gtagtgactt agaaaaatgt tttaaaatag ctacagagaa taaatgcaat
     4741 agattgcgtt taaatatgca tgatattgaa tctattaaga gttttattga gcaaataaat
     4801 atttccgaat ccaatactga atttggaaat gaaatattga gaaatacatt atttatacaa
     4861 cttatgatat tcttaaacag aattttccta aaaaatgaaa atatggaatc aacagaagat
     4921 gttttttatg atgaaactat taagaaagta ttaaactata taaattcaaa tatagatgat
     4981 aagctttcaa tagatagcat atcgtctgaa ttttttataa gtaagtatta tcttatgaga
     5041 aaatttaaat ctcatactgg aacgtctata cacaattata tagttcaaaa aagattaatt
     5101 ttggcaaagt cactaataac caatggtcac cttatgagtg aagtttgtaa taaatgtgga
     5161 ttcaacgatt attctagttt tgtaagggca tttaaaaaaa actacggtgt atctcctaaa
     5221 aattatataa acaataaaaa tcctttagat attactcttt tagatgaata gataatttat
     5281 gtaaatttta ttttaataaa acttgaagta ttagtgtatt tatcttacta atacctcaag
     5341 ttttttaatg ttttaaaata tatcaaaata taatgatata tatttctatt acttaaaata
     5401 tatattaaat aaattgcatg ttaactgaac ctatttatat atttattaaa tctcacataa
     5461 cttttagtta cactatcata tagctatcct attttttcaa tatcatctct taatatactt
     5521 actctattct cctcttttgc tgtggttact ccttttttaa tgcttaattt ctgattttgt
     5581 tctaaattcg taaatactac aggacttatt gttgatagac catttttttc aatatattct
     5641 aagtccattt taactaattt ttctcccgct tttacttcat ctcctacatt cacaaatact
     5701 tcaaatccat ttccttctaa gtttacagtt tctatcccta aatgtataag tatttctact
     5761 ccactttttt gggattttat tattattgca tgcttagttg gaaatataac tccaatagtt
     5821 ccatcaactg gtgaataaat caatccatca ctagatttaa ttgcaaatcc atcccccatc
     5881 agtttacttg aaaatacaga atctggcact tcttctatat ctaataactc tccatttgtt
     5941 aatgaatcaa tcttttctac taaagtattt tccatatttg caacttccat attttttttc
     6001 actttatttt tatcaacatt tttagtaaca gtactttcaa cctttgtttt tccctctatt
     6061 atatctttta tatcagactt tattgaatct gattttggtc caaatatagc ttgaacattg
     6121 ttgccaataa ccataacacc tgctgctcca agtgatttca atttttcttt atctacttta
     6181 tttatatcat ttacaaccaa tctaagtctt gttatacacg catctagact ctttaagttg
     6241 tctttatttc ctaaagcaat caaaatctct gatgctaatt cattcttaga catttcaatg
     6301 acttcagcgt tagtttctga ctcatcttct cttcctggtg tctttaaatt aaattttctt
     6361 atcataaatc taaatacact atagtataat actgcaaaac caatacctac caagatagcc
     6421 aagtaccatg gagttctatt aggaagaaca ccaaataata agaaatctat taatcctcct
     6481 gaaaatgtga atccaatttt aactcctaat aattgcataa ttgcgtatga taaacctgaa
     6541 aataaacaat gtattgcaaa taatactggt gcaacaaata aaaacgcaaa ctctattggc
     6601 tcggttattc ctgttaaaaa agaggttaat gctgctgatg ccattatacc tgctacaaat
     6661 tttttctttt ctggctttgc ttcatgcacc atagcaagag ctgctgctgg aagaccaaac
     6721 atcataaatg gaaatgcacc tgtcataaaa gtacctgctg tgaaatttac tccatcttta
     6781 agttgagcaa agaatatctt ttggtcaccc attacaagct caccagcttt atttacatat
     6841 tctccaaatt gataccaaaa tggattatgg aaaacatgat gcaaaccaaa tggtattaat
     6901 agtctttcca aagaaccttc cataaatgct gctagtgttt gatttgcttc tataacattt
     6961 tttgagaaac taagtaatcc ctcttgtatt ggaggccaaa taaaagacaa tataattcct
     7021 attgctatag caactaatga agtaacgatt ggtacaaatc ttttacctgc aaaaaaacct
     7081 agatactgag gtaattcaat tttataaaac tttttataaa gataagcagc cactatccct
     7141 ataataatac caccaaatac ccctgtttga attgttggta ttccaacaat atttgcatac
     7201 atagaattgt ctaccatatc ttctgtaaca cctgccataa cacccataga tgtattgaga
     7261 attaaaaaac ctactatggc tgccaatgct gcaacacctt ctccttcagt taaaccaaca
     7321 gctaccccaa ctgcaaatat aagtggtaag ttattaaata taatttgacc tgaactttcc
     7381 attatactta ttataagttg aaatgtagga ttcgccaaag ctggaaaatg agccaacaat
     7441 gcgtctcctg ctaaagcatt actcacgcca agtaatatac ctgctgcagg aagtaatgca
     7501 actggtagca ttagagattt tcctactttt tgtaaaacac caaatacttt tttcatttac
     7561 ttttctctcc ttattatttt atattttttt aaataaaaaa gacttaagtt tataaaaagt
     7621 tacattagta tacttaattc taaaattaaa cttatgttaa tataaacaaa cctatattaa
     7681 cctaatttca gaattaaaaa taaactatta taattttttt ataaacataa gtcttgcctg
     7741 cattatcagt aacaatctta atgttttaaa attattattt tatattaaat tttaaataat
     7801 atattaaaat taaacataat atattaaatc tatacattct gtattaaact actctttatt
     7861 ataaattcag tacagatata actcttttga tatgtaatgt taaataaagt agttcatctt
     7921 gacttacata gtaatcataa ttcttttcta catgagtttt tattttatat gcacaatcat
     7981 aatccttttc atactttact ttaattatat ctataatttc attatttgta tcattgattt
     8041 tttctttttt tactaatcgt tttgcaaaga attttaaatg agtcagaagc ctatcataat
     8101 ttatatcatc ttctttaaat tcaactctat aataatatct tataatattt agtatgtctt
     8161 ttacaatttt agtaattaac agtgattcct ttgaacttcc cttatagttt gaatttacta
     8221 tatgcattgc tataaaacca gcttcatcta ctggaaattt cacatcaaat tctttattta
     8281 tataatctac agcccatttc ccaatctcat attctttttt atgtattctt tttatctcat
     8341 gtagtaaatc attctttata gttatgtttt catgatatct tttaattgca aatgcaatat
     8401 ggtcagtaag tgctacataa atatattcat aaagtttagt attcaaattc tcctctgcat
     8461 aagcaattat ttcagtacta acctcaaaaa atttctcatc aacctggctt gctagtttac
     8521 taattttatc taaaaagctt tcatcttgta ttacaaaagt tctttcaatt ttacttttat
     8581 ctatttcttg acctttcttt ttttgaaacc caattccaca tcctgttaat attaattctt
     8641 tttttatatt ttcatctaaa actaatacaa cattattatt ataaatttgt tgaataatca
     8701 taatcaatat ctctcccaaa atacaatttc ccttattcat aaaaaaatac ctaaattaag
     8761 atacaattca cctgtatacc ttaacctagg tattgcctgc ttaccagtaa caatccagct
     8821 tgttaacgct tatatattgt attcaattta tggttttatt ctattataaa taaatatata
     8881 tgtcaagtaa attattgaaa actatttcct attttttagt attaatttta acatcaaaaa
     8941 tcaaatttta ttccattata tttatgattc tatcatcatc atttcatcat agagaatatt
     9001 ttccaataac tctttagcca cttctcttga tatttgctta gtttcaacta attgttgaac
     9061 actgtctctt tgactttgta tcgccattac tctgtagtat tctactcttg cctcaaactt
     9121 atcatcattt tgaatctttc tagtaatcca attcaaataa cgattataga atctgattgc
     9181 tattaaggcc tcatgtctgt tatcttcatc agtattttta tttataaatg atatagcagt
     9241 tttagtagaa agtgcatatg catggtgaaa ctctttgatt atttctttat tctcagatac
     9301 tatttttgta attttttctt ttacttcttt ctttactttt ctatttataa tccacatgct
     9361 tatttttaat tttacaattt taaatataga tttttgaaca gattttcttg attttgtaat
     9421 ataaaatcta tacaatttaa aggctctatc agaaatttta ccatcttcta atagtttcaa
     9481 aacagctaat aatccctgtt ggtctgcttc tgcaatcatc atacttataa gtttattatc
     9541 tggagtattt agtattcctt tttctgagtc taccaactgt tcatttaaat catgtataac
     9601 tggttgtact tcttgtgctt tttcattact aagttttaaa atggcttctt tcaaaattag
     9661 tttatatgcc tctttatcag ataataaagg ttctgactct tgtacgttat tttttaataa
     9721 tcttggcagt aaaactgttg ccaatactaa acttataaga ataacttctg atgatataaa
     9781 taaaacagta tctctcattg gaaatacatc tttatcagcc aaataaaaag gtatagataa
     9841 ggcagttgct agtgtaaatg taccatgaac accacaagta gcaactatta gtgagtattt
     9901 acatttagaa atactctctt ttacaacttc ttcatcctta aaacctaaaa aacctacaaa
     9961 aaaattattt aaaggattct ttttaggttt tataaaagaa ttatgccata aatataccca
    10021 tataaatctt ataacaaata aaataagtac aattaaagta gaaataaaag tcaattccaa
    10081 tgccatattt tcatttttac tactaagtcc tgtatatata cttggtaaca aaaaaccaag
    10141 cagtgtaaag acaaatccat tcaatacata ctccaaaaca taccaagtat tgtctgatat
    10201 tatccttaat ttagtagttg tattttgtaa atgctctttt tcaattccat gagctatccc
    10261 tgccattaca acagctagga ttccagatac tcctatttct tctgctacaa aatatacaaa
    10321 aagtggagtc attatttgta taactattac cattggaatt tcttccaaat tccacttgtg
    10381 taaactaagt cttattttta taattatgta tcctaaaata cttcctacta ttattcctcc
    10441 aaaagctgta atcataaatt gaatccctgc ctcttctatt gaaaaaactc ctgtaactgt
    10501 ggcaagtact gcaaccttaa atgctactac tccagcagca tcatttaata aagattcacc
    10561 ttcaagtata ctcatcatat ttttaggaaa atttaatcct tgtgtaattg attttactgc
    10621 tactaaatca gttggagata ttactgctgc caaagcaaat gaaacagata atggcatccc
    10681 aggaataaga aaatgtataa agattccacc tgcaaacacg gttattaaaa ctaaaccaaa
    10741 tgcaagaagc aaaattggtt ttctgagctc taatagctct tttctcgata cattttgtcc
    10801 ttcactgaat agtaatggag ctatgataca tattacaaat atttctggat taaatgaaaa
    10861 tgagaattta aatggcaaaa tagacaatat aatccctagt acaatttgat atattgcaag
    10921 aggaatatta ctccactttt tgctaaatac agttgaaatc gttgtacaaa ccaataatcc
    10981 caacaatgtt actaaaattg acataactac ctctcttttc aataataata ttatagatta
    11041 aacttatata tcaactaact taatattaac agcatgaaat cccatcttaa tcaaaaaaat
    11101 tttcagttta ttctattatt cttattatat acatcaaatt taatttttga aatatctata
    11161 ctagatgaca tgcaacaaaa tgatttcttg atatttcttt taaaactgga atctcttttt
    11221 tgcatatttc cttacaataa gggcatcttg tatgaaactt acatccactt ggtggatttg
    11281 ttggagatgg tatgtctcct tttaatataa ttctattttt cttaattgta gggtcttgta
    11341 ttggtactgc gctcataagc gcttttgtat atggatgttg agaatgtaag taaagttctc
    11401 ctttagttgc aatttctatt atagaaccta gatacataac acaaactcta tgtgcaatat
    11461 gatttactac actcaaatca tgagatataa ataaatatga aagtttatat ttatcctgta
    11521 aatccataag taaatttata atttgagatt gaatagaaac atctagtgca gaaacaggtt
    11581 catcacaaac tataaattct ggatttaatg ctaatgctct tgctatacct attctctgtc
    11641 tttgacctcc agaaaactca cctggttttc tatttaaatg gtacttagaa agaccacaac
    11701 tttctacaac atttaatata ttatctttta tttcattctt tggcactaaa ttatgttcta
    11761 taactgcctc ggatattatt tgttccacat ttaatcttgg atttaatgaa ctatatgggt
    11821 cttgaaatat catttgcatt ttgggtctta actttctaag ttcttccttc tctaacttat
    11881 atatatctac accattaaat tcaactttac cttcagtttt ttcaattaat ctaagaatgg
    11941 ttcttcctgc agttgatttt ccacatcccg attctccaac tatagccaaa gtttctcctt
    12001 tttttaaatc aaaagaaatt ccatctactg cctttacaca tttaatttcc ttatcaaata
    12061 atccagattt aacttcaaag tactttttaa gaccttctac ttttaataaa ctctcttctt
    12121 ttttcaatta tatctcctcc tttttatata accaacatgc aatactatga ccactattca
    12181 attctattaa aggaggtatc tgagttctac atttatcaca tactttttca catctatcac
    12241 taaaataaca tgaatctggc atcccaattg gatttggtac ttgtcctgga atcgaataca
    12301 atcttttatc tttgtttttt ccaagagcag gctttgattt tagaagtcca atagtataag
    12361 gatgttttgg atttttaaat aattcaacaa cctctgccac ttctataatt ttgccagcat
    12421 acatcactaa tactttgtca cacatttctg caactactcc caaatcatgt gtaatcatca
    12481 ttatagatgt gtttaatttt tcttttatat ttttcattat atctaaaatc tgagcttgaa
    12541 tagtaacatc taaagctgtt gttggttcat cagctataag taattttgga ttacatgata
    12601 aagccatagc tatcattatt cgttgtctca ttccacctga taattcatgt ggatatgaat
    12661 tatatatttc ttcagctctt ggtatttcta ccagttttat catatcgatt acctttttct
    12721 tagcttcttc ctttgataaa tcttgatgta tcataatacc ttcaatgatt tgacttccaa
    12781 tagtaaacgc tgggtttaaa gatgtcattg gttcttgaaa aatcattgat atctcattcc
    12841 ctcttattcc cataagttca tcttcattca tctttaaaat atctttgcct cgaaagatta
    12901 tttctccacc ttctattttt gatgttggaa gtaacttcat caaagacatg gctgttacag
    12961 acttaccaca tcctgattct ccaactattc caatagtttc accttcattt atattaaaac
    13021 ttacttcatt tacactcttt acaatccctt tatctgtatg aaaataaact tttaaatctt
    13081 taacctcaat tagtgcttta ctcattattt tctcctctca gcaaaattac ttaagataac
    13141 atacaccttt tgattgtatt ttataaaaat ttaaattaca acattttctt acattttatt
    13201 atttttttct attaattata ataatataat agttaatttc tatatatatt gatttaataa
    13261 tgattactat tatacaataa aaaatatgtg ttaaatatta ttttatggag gttttataag
    13321 aatatgaaat ttaaaaaatt ggcatcatta atcttagttt catcattaat gcttacattt
    13381 acagcctgtg cttccaatga taaagacaag tctagtggaa gcaaatctgg tggaactgtt
    13441 gtaataccta tggcaagtga cccagatata atcaatggag cttttgcaaa cgttaaagaa
    13501 gactctcttg catcaaatat agtttatgct cctctttata cttatgaaaa aggaaattta
    13561 gtaaattatt tagcagaaaa agtagatttt aaagatagta aggaattaac tataaaatta
    13621 aaatccaatt taaaatggca tgatggaaag ccaataactg ctgaagatgt cttatttaca
    13681 tttaatacag ttttagacga aaaacaaaat tcaccatcaa gacaatactt attggtgggt
    13741 gagaaaccag ttaaagtaga aaaaattgat gatttaacag ttaagattac tttaccaact
    13801 gcaagtgaat ctttcttata tggtatatct aaaatttctc caataccaaa acatgtgttt
    13861 gaaggagaat caaatatagc taagtcagaa aaaaataata atccagttgg ttctggtgca
    13921 ttcaagttta aggaatggaa aaaaggtgaa agcatagtat ttgaaaagaa tgcagattat
    13981 tttggtggag aatctaaggc agattctata gcgctaaaaa taattcctaa tgaagcatct
    14041 caagaagcag ctttaaataa tggcgaaatt tcacttatga agacatctgc tgaaggatat
    14101 gaaaaggcaa aatcaaactc aaatttacaa acttatactt atagtgaaga aagattaaac
    14161 tacattgtat ttaaccaaaa tatatcaaac atggctaaca aagaagtaag acaagcctta
    14221 tcatatgcct taaatagaaa tgaaatgata gaatcagcat atggaaaaga aggttctgta
    14281 cctgctaaat ctatattagt tccagaagca gacttctata cagaagaagg tgttgaagga
    14341 tatgaccaag atacaaataa ggctaaagac ttattggata aaagtggtgt taaaattgat
    14401 aaattaaaaa taggttataa cacaggtaga tttgggcata aaaactatgc cttagtagct
    14461 caacaagagt tgaaaaaaat tggtatagaa gctgaaatag ttccttatga aagtaaggca
    14521 ttcttcaata tactatttag taatagcact gaatgtgata tgtatgtaaa tggttatgct
    14581 tggggattag aaccaaaccc atatagaggt atgtttgaaa ctggtcaata ttgcaaccaa
    14641 actaaatatt ctaatgctga aatagatgct ttatgggaaa aaggatttac tgagttaaac
    14701 aaggaaaaac gtgaagaaat atataagcaa atacaacagg atatatctaa ggatgcgcct
    14761 atttacacta tagattatga acaaaattta atggcagctc aaaaaaatct aaaaggaata
    14821 aaagatgcta aaccatctcc tgcaatatta tttgaagatt ggtctaaact gtatgtagaa
    14881 taattgatgt aaaattttat attattaatt agaaaaggag atgtctaaaa ataatattgt
    14941 tttattattt taaaacgaga catctctttt aattaggaga atttacttat gttaaaatta
    15001 atattgaata gagtaaaagt tttaatacct atgttaatct taatatctat attatcattt
    15061 ggacttttag aattagctcc tggtgaccca gcagatgcat atataaatcc tttaatgtct
    15121 tcacaggata ttgaaaatat aagggttaat atggggcttg ataaaccagc atatataaga
    15181 tatttaaact ggttgaaaaa cactttaagt ggaaacttag gtatttctta tataaaccat
    15241 atgcccgtta cagaacaaat tatggaaaaa atgggaaata catttattct tatgggtact
    15301 tctttgatat tttcaatatt ggtagccata cctcttggaa tatttttagc tgtaaataaa
    15361 aattcaataa ctagtaaagt atcatctgtt tttaattata taggtgtttc tataccttct
    15421 ttttggatag gtatgatact catttcaatt ttttcagtta aacttaacat tttcccatca
    15481 ggaggtatgc atactattgg aaatgatagt attggagatt tagcaaaaca tttagttttg
    15541 cctgtaataa ctttaggtct atataatact gctattttta caaattatgt agaagctagt
    15601 gtaaatgaac aattaaaaaa acaatatgtt attacagcaa gagcaaaagg attatcagaa
    15661 aaagtcatac tatttaaaca tgtcttgaaa aattcactga catcattggt tacaatccta
    15721 ggcatgagta tacaaaaatt agtcactggt gcatttgtta cagaagtagt tttctcttgg
    15781 ccaggtatgg gaagattaat gattgattca attttttcaa gagattatac tgtaattatg
    15841 gcaataacta tgttatcagc actattttta attttaggta atttagttgc tgatatacta
    15901 tacttattaa ttgaccctaa aataaaatct agcaaaggag gattctagtt gtgtttacaa
    15961 gtatcttaaa aaaattaaaa aaagataaat cctttgcttt tgcatgtatt actcttagca
    16021 taataatcat attaattata gcttctttta tattaaatat agactctaat gcaattgata
    16081 ctacttctaa acttcaagaa cctagcttaa agcatatctt tggtacagac gaacttggta
    16141 gagattactt ctctagagct ctttatggtg gaaggatttc acttagtgtt ggtattttat
    16201 caatgcttat ttctgtagta tttggtactg ctgtaggtgt tattagtgga tatattggag
    16261 gtaagctaga ccaattcttg atgagattag tggatatgct tatgagtata ccaagtttct
    16321 tagttttgat tgtattaaat acttatctaa cacctaaagt ttccactatt atactaatta
    16381 ttggattctt aagttggatg gaagtttcaa gaattgtacg tggcgaaaca ttaaaaataa
    16441 aggaaaatga atactgtcta gctgctaaag ctactggtat caaaactaga aatatactat
    16501 taagacatat agttttaaac ttaaaagaga ctattgtagt tgctggttcg ttaaatattg
    16561 ctaatgctat cttaactgaa tctgctctta gttttttagg tcttggaatc caattgcctc
    16621 ttgcatcatg gggtaatatg cttcaagatg ctcaaagaca tatttttgac aagttatatt
    16681 tagctgtatt tccaggatta cttatatttt taacagtcat gagtttgact ataataagca
    16741 aaaaattttc tagtaatact taacaaattt tatatagaaa ataaaaacag tgattatcta
    16801 aagatataca ctgtttttat ttttcgttta aaaaaataca tattttgtat attataactt
    16861 taaatactac tctaccatgt tatgcaaagc gtattattgc tctcaaacat atagatgaaa
    16921 aactaatagt ttattataat aaaaattgat ttatttattt gttattttaa taacattttt
    16981 aagagttctt tttactaaag aaatctataa taaatttaaa tacatgttgg tctacatggc
    17041 tataacgttc ttttttaggt ctacataata taactttcca tgtaatagga tgattaaaag
    17101 gcttttctat aattttgcta ttttcaaaaa aatcatgaac tggtgatggt aaaattgtta
    17161 taaaatcaga atttgtggtt gatagcaaca aaaaatccca tgaaccagac attatatatc
    17221 tttttaactt aacattttca tcagtaaatt tttgcattag cttatgatgt atcataaaag
    17281 taggattaaa tattgcaagt aactgctcat tcaaatcgtt ccaatctatc ttatccttct
    17341 tagcaagtgg atgatttata tttaaaaatg ctgtaagttc atcttcttga agcacatgtt
    17401 cagttactac acttttatca attttatgtg gatgtaacaa tactgcataa tctaattcct
    17461 gtaagataag cattctattt aagtcatatg cacccttctc tactatatca aattctatat
    17521 ctggattatt tgcaactaac tgtgcaacta catctgaaaa tgctattcca agtataagtg
    17581 gaggtattcc tattctaact tttcctttaa attgaactga atcttctctt aaatcttcca
    17641 tcatattttt gtattcattt atgatatgtt ctgcatttac ataaaatctt tctcctgctg
    17701 gtgttaaccc atttaacctg cctttatacc tttcaaataa atatacatct tcactttctt
    17761 caaaattttt tataatttga cttaatgctg gttgagatat gtgaagtgtc ttagatgcta
    17821 ttgataaatt aaaaccagat ttcactattt caacaaaata tttcaactgt tttatatcca
    17881 ttttaaaccc ccttccccaa aagtataatt tagattatat tttttcttct aatgatattt
    17941 tataggaaaa tcaacataat ttcattaata ttttaacaaa atatttttct aaatctacta
    18001 taaattttta tcctataaat ttttcttata cactatataa aaaattagac ttagacaaaa
    18061 cgcttttttc gccgtaatct ataattagaa acaggaaaac ctaaagcatt aggaataaaa
    18121 gtaattgaaa tacttaaaaa caagcttgta ttaaaaaagt actttattat agtgttccct
    18181 aatttcttta ttcagaaaat ttttaattat cattcaagaa aggtaactat tagatatgaa
    18241 agatgtagta atcgtatcag cagtcagaac acctatagga agttttggag gtgtatttaa
    18301 aaatacttca gctgttcaac ttggaactat cgctgttaaa gaagcgatat cacgtgtaag
    18361 acttaattta tctgaaatag atgaagtaat aattggaaat gttcttcaaa cagggcttgg
    18421 acagaatgtt gctagacaaa ttgctataaa tgcaggtata ccaaattctg ttccaagcta
    18481 cacagtaaat aaattatgtg gttcagggct taaaagtgtc cagctagctg cccaatcaat
    18541 cacatctggt gaaaatgatg tggtaattgc aggtggtact gaaaatatga gccaagctcc
    18601 ttatatcgtt ccaactgctc gctttggtag taaaatgggt gacataacca tggtagatag
    18661 tatgctaaca gatggcctta ttgatgcttt caatcagtat catatgggaa taactgcaga
    18721 aaatattgca actaaatttg aatttactag agaaatgcaa gataaattgg cattagaaag
    18781 tcaaaataag gctgagaatg ctataaaaaa caaccgcttt aaagaagaaa tcgttccagt
    18841 agatgtttta atacgtcgag gaaaaataga aactattgat aaagatgaat atcctaaatt
    18901 aggtatgaca tttgaaggtt tatctaaact taaacctgca ttcaaaaaag atggtactgt
    18961 aacagctgga aatgcctcag gtattaatga tggtgcggct atgttaatac taatgagcca
    19021 acaaaaagca gacgaactag gtattagacc acttgctaaa ataaaatcct atgcttcagc
    19081 tggtgttgaa cctgaagtca tgggtactgg tccaatccct gcaactagaa aagcactgaa
    19141 aaaagctgga cttagcatca atgatataga cttaatagaa gcaaatgaag cgtttgctgc
    19201 ccaagcatta gctgttaaaa atgaacttca aatagattca tcaaagttaa atgttaatgg
    19261 tggtgcaata gcattaggac atccaatagg agcaagtgga gctagaatac ttgttacctt
    19321 aatttatgaa atgcaaaaac gtaaagttga aactggactt gcaacacttt gtattggtgg
    19381 tggtcaagga atatcaatgg tggtttcacg ataggaggaa atatgaataa aatagttagc
    19441 attgatgaag ctctatctca tgtaaaagat ggagatacaa taatggttgg tggattcatg
    19501 gccaatggtt caccagaaaa tttaattgat tatttatgta gtaaaaatat aaaagatttg
    19561 acactaattt gcaatgatac tggctttatt gacaaaggag ttggcaaaat ggttgtaaat
    19621 aagcaattta aaaagataat tgcttctcat gtaggattaa ataaagaaac tggaagacaa
    19681 atgaataaca atgaaacaga tgttgagtta gttcctcagg gaactcttgc tgaacaaata
    19741 cgtgcagctg gttatggtct aggaggtata ctcacaccga ctggtattgg tactcttgtt
    19801 gaggagggaa agcaaaaaat tgttgtcgat agcaaagaat accttcttga aaaaccaatt
    19861 tatgcagatg ttgctcttct atttgcatca aaggtcgata aagcaggtaa tctagtttat
    19921 aaaggctcta tgaacaattt taataattta atggcatctg ctgccaaaat tacaatagtt
    19981 gaagcagatg aaatcgttga aattggtgat attgacccaa atgaagttaa tacaccaggt
    20041 atttttgtga attacatcgt tgaaggaggt aatttataat ggataaatta gaaatgcaag
    20101 aatatattgc aaatagagta tctaaagaat taaaagacgg agccgttgta aaccttggaa
    20161 taggtctacc aaccaaagta gcaaattata ttccggataa tgtaaacgtt atcttacaat
    20221 ctgaaaatgg ctttttaggt ttaggttcag ccgaagatgg aaattcaagt gatgaaacta
    20281 ttgtaaatgc aggtggtcaa ccagttacta tacttcctgg aggatgtttc tttgatagtg
    20341 ctacttcttt tggaataatc agaggaggtc atgttgatat aactgttctt ggcgcacttc
    20401 aggttgacaa atatggcaat atagcaaact atatgattcc aggtaagatg gttccaggta
    20461 tgggtggagc tatggaccta gtaactggag ctaaaaaagt tattgtagca atggaacata
    20521 cttcaaaagg ttctgcaaaa atattaaata attgtactct tccacttact gcaacaaatg
    20581 ctgtagattt gatagttact gaaatgggag ttatggaagt aacatcagat ggaattttac
    20641 ttaaagaaat aaatccagct ttcacattag atgatgttat ttctgctact gaagctcctt
    20701 taatattgtc agattcatta aatggaaaaa tatctgttgt ttaaaataat taaaactaga
    20761 aaataaaaat ctcttagacg tattacatat aaatttgtat tgcttaaaat attaatataa
    20821 aaataaaata aaattggagg aaatgaaaat ggttaaagat aaagttgtat tcgtaacagg
    20881 agctgcaagt ggaataggta aacaaatagc tgaatcgttc ttaaaaaatg gtgctaaagt
    20941 aatgttctct gatataaatc aagaagcttt aaataatacc ataacagagt tacaaaaaaa
    21001 aggttatgat tgcatgagtg taaagtgtga tgtaactaag gaagaagaaa ttaaccacgc
    21061 tatagacaaa actgttgaaa agtatggcag attagatata ttaataaaca atgctggact
    21121 tcaacatgtt tctatgatag aagatttccc aactgataaa tttgaattta tgataaaaat
    21181 aatgctgaca gcacctttta tagctactaa aaaagcattt cctataatga aaaaacaagg
    21241 ctttggtcgt ataataaata tggcctcaat caatggtgta ataggatttg ctggaaaggc
    21301 tgcttataac tcagcaaaac atggacttat tggactaaca aaagttgctg ctcttgaagc
    21361 tgccgattct ggtataacag ttaatgcttt atgccctgga tatgtagata ctccacttgt
    21421 cagaggtcag ttcaatgacc tttcaaaaac tcgtaatata ccattggaaa atgtcttaga
    21481 ggaagtttta tacccacttg taccacaaaa acgcctatta gatgttcaag aaattgccga
    21541 ctatgctatg ttcttagcaa gtgataatgc taagggtatt acaggacaag cttgcttact
    21601 agatggagga tatactgcac aatagttatt atatttaggg cttgaagaaa gtattcttca
    21661 ggtccttcta ctaaggaggg atatttatga gtattatcgc gccaattggt ataattattg
    21721 gtatcatagc tattatatat ttttcagtta aagaaattca tataactata gctgctccaa
    21781 tagcaacttt aatagttgtt attttaaata aaatggatgt tgtaacgtca atgctaggtg
    21841 ctgacaaaaa caattatatg ggtgcacttg gaaactatat aatgagttat tttgcaatat
    21901 tcttattagg ctcaatactt gctaaattta tggaagaaag tggagctact gtatcaatag
    21961 ctgattttat tttaaataaa tttggctcta atcatcctta tagagtttta gttgctatat
    22021 ttatagtaag ttttatatta acgtatggtg gtataagctt atttgtagtt atgtttgcgg
    22081 tcattccact tgcaagaact ctatttaaaa aacttgatat ttcttggaac cttattcaga
    22141 taccactttg gcttggtatt gcaacaatta ctatgactat aattcctgga acaccagcca
    22201 ttcaaaatgt tatacctatt caatatctaa atacgtctct tactgcagct actataccaa
    22261 gtattgtagg tagtataggt tgtgctacat ttggacttgt ctatatgaaa tatgctctaa
    22321 acaaaagtat tagaaaaggt gaaacttatg caacttacac ttcagaatta gaagaagaat
    22381 ctttagatag agaattacca ggattttttg caagtatttt tcctctatta gcactcgtaa
    22441 ttatagctat aactggaagt acttttggtt cagaatttat gaaaaaaaat gtaatttaca
    22501 tagctttaat agttgcaatt cttttatctg cttttttatt ccataaattt ctatcatcta
    22561 agatacagac attgagcata ggtgctagtg gttctatagg tcctatattt gctacatctt
    22621 ctgcagttgc atttggagca gtaataatgg ctgcacctgg ttttgatgta tttagcaaac
    22681 ttataatgtc aataccaggt agtccactta taagtcttac tgttttgact tcaacaatgt
    22741 cagcaataac agggtcctct tctggtgcat taggaatagt aatgccaaac tttgcagatt
    22801 attatttaag tactggtcta aatccagaat taattcaccg tgttgctaca atagcttcaa
    22861 atatactaac tatagttcca caaagtggtg tttttcttac attcttaagc ttatctaagt
    22921 taacacataa aactggattt aaagaaactt tcattgtagt tgcagttggt tcactaatag
    22981 cagaaattat tgtaattaca ttaggattac taatgtatta atataaaaca agttaaaatt
    23041 cataatatga aaggtggtgt ctcaaagtga actttttact taatgagaca ctctttttat
    23101 atgaaataac atatgcctat catttaaaca ataaaaaagg ccttatctct attttgagac
    23161 agaccctttt tatttcttaa agatttaaat aattataatt tgttgctaat acgaaagcat
    23221 ctactgaata aatttaacca aataaaaaaa aatttatcat aactataaat ttacgttatg
    23281 gaattattat aaaaatatta ttttctctaa ctaagtaatt ccatactata caggctttta
    23341 atacaaattg tcaaacacac tgaaatatac ctatttattc catatatatc ttttttacat
    23401 atctttacag taaaaaaagt cttaatctta tgttagtata gattcacggg taacccccta
    23461 cactaaaggt tacaaatcag taacattttt agaattttta gattaagagg tgtttaatga
    23521 tgagaagaaa tataccaaca aaattaaaaa ctatattagc tttaggagtg ttaactttag
    23581 caacagcttc aagtgctgtt actgcaagtg cttcatcact tgaaaataat gtaaatataa
    23641 gtaacaaaag tttagtatat agtaatacta aatacaagtt ttctgcaaaa ccaaattgtt
    23701 ctgtaaaacc agaacttaag cctgataaag ataactcttg taaggataag aatcataatg
    23761 acaagaattg caacaacact aataaacctg aggacaacaa taattcagac tctactaata
    23821 agcctgataa caataaacct gagtctaata aacctgaaga caacaataat tcaggctcta
    23881 ctaataaacc taataataat aaacctgagt ctaataaacc tgaagacaac aataattcag
    23941 gctctactga taaacctgat aacaataaac ctgagtctaa taaacctgaa gacaacaata
    24001 acaataattc agactctact aacaaacctg atgataataa caactcaggc tctacaagtg
    24061 aaagtttttc agcttaccaa aaagaagttg ttgacttagt aaatatagaa agagctaaag
    24121 ctggattaaa tcctcttact cttgattctt ctatatcaaa tgttgctaca aaaaaatctc
    24181 aagatatgat tgataacaac tatttttcac acaattctcc aacttatggt tctccatttg
    24241 acatgttgaa aaaatttgga ataagctata agacagcagg tgaaaatata gctatgggtc
    24301 aaaaaactcc taaagaagtt gtaaacgcat ggatgaattc tgaaggtcac agaaaaaata
    24361 taatgaatcc aaatttcagt aaaataggtg ttggagtagc acaaaaaagt ggtggttcaa
    24421 tttattggac acaaatcttt gtaggataaa atactctttt tccatacata tcaaaagagt
    24481 atctctaagt gataaaaaac acaaatgaga tactcttttg tatctatttt aaaattatca
    24541 aatccaaata tacatactat aaatattatt tgttctctta attttagtgt gtagaacaat
    24601 atataaaaag tattggcaag atgaaaatgg aaaacttaaa aaagatattt aataggtaaa
    24661 aacaaaatct taatatcttc ataaaaaaag gctgtctcaa aatagagata aggccttttt
    24721 cattgttgaa atgattaaaa tatatttgat ttcatacaaa aagagtgcct catgaactaa
    24781 aaagctcact ttgagacact ctcttttatc aatttatata agcattataa tactttaata
    24841 ttattcagct aattttttgt atccttcata tctttcttta gcatcagctt cagctttagc
    24901 aaataatcca tcagcttctt ctgggaattg tttagctata gcagaatatc ttacttgtgc
    24961 aagtatgaaa tctttgaagc ttgcagttgg ttcttttgaa tctaaagtga atgggttttt
    25021 accttcagct tttacttctg gattgaatct gtataaatgc cagtatccgc aagcaacagc
    25081 ttgtgcttcg ttagctacag ttcttcccat accttctttt aatccatggt ttatacatgg
    25141 agcataagct attattagag atggcccatc atgtttttca gcttctataa cggctttgat
    25201 aaattggttt ttatcagcac ccatagcaac ttgagctaca tatacgtttc catatgtcat
    25261 agccatcatt cctaaatctt tctttctaga ttttttacct gaagcagcaa acttagccat
    25321 agcagcagct ggagtagctt tagaagcttg acctcctgta tttgaatata cttctgtatc
    25381 aaatacaagt acgtttacat cttcaccaga tgcaagaaca tggtctaatc caccgtaacc
    25441 gatatcatat gcccaaccgt ctccacctag tatccattga gatctcttaa ctagataatc
    25501 ttttctatct tttatagctt ttactaactc tttagctttt tcatctgtac aatcacaagc
    25561 tggtttttct aataattcaa caaccttagc acttgctact ttagaagctt ctccatcatt
    25621 tctagaatct aaccactctg taaatactgc tttagcatct tcacagatac ccatagaaac
    25681 taactctgtc atagcatcta ctattttatt tcttatttgc ttaacagctg tgtacatacc
    25741 aaatccatat tcagcattat cctcgaataa agagtttgcc caagatggac ctttaccttc
    25801 atggttacaa gtataaggaa ttgatggtgc agaagctccc catatagaag aacatccagt
    25861 agcattagct atcatcattc tatctccata taattgtgta actactttaa tgtatgcagt
    25921 ttctccacat ccagcacatg ctcctgaaaa ctccattaat ggttgtctaa attgacttcc
    25981 tttaacagtg tttacaggca taacatcacc ttttggagct acctcatcag gatttactgc
    26041 aaatgcccaa ttatctaact ctgtatcaag ttgagtatct attggcttca taactaaagc
    26101 tttttcttta gctggacata tgtcagcaca gtttccacat cctgtacagt caagtggact
    26161 aagttgcatt ctatatttta atccttctaa tcctttacct aatgcttttt tagcttcaaa
    26221 tccttctgga gcattagcta attcttcttc agtaactaat actggtctta tacatgaatg
    26281 aggacatata aaagaacatt ggttacattg aatacagtta tctattatcc attcaggaac
    26341 atcaacagct ataccacgtt tttcataagc tgcagtacca caagggaatg ttccatcttc
    26401 tataccattg aatgtactta ctggtaagtt gtttccttct tgtgcagtca ttggtcttag
    26461 tatatttttt ataaattctg gttctttaac ttctccttta tctgcatcaa cagcattttc
    26521 ccaagaagct ggaacttcaa ctttaactaa agcatttatt cctgcatcaa cagcagcata
    26581 gttcatgtta actatttttt cacctttttt accataagct ttaactatag aatcttttaa
    26641 gtatttaaca gcatcttctt ctggtattat ttcagctaat ttaaagaatg cagattgcat
    26701 tatcatattt attctgtttc caagacctat ttcttgtcct agtttaacag cgttaactgt
    26761 atagaatttt atatcatttt tagctatata ttgcttcata tgagctggta aatgttcttc
    26821 aagtcctgct tggtcccata tagtgttaag tacaaatgta ccaccttttt taagaccttt
    26881 taataaatca tattgataaa catatgattg tttatgacag gcaatataat cagcttcatc
    26941 tattaaataa gtagctctta ttggagtatc accaaatctt aagtgagaca tagttatacc
    27001 accagatttt ttagagtcat aatcaaagta agcttgagca tatttttcag tatggtcacc
    27061 aattattttg atagcttgtt tattagctcc aacagtaccg tcagaaccta atccccagaa
    27121 cttacatctt atagttcctt tagaagctat ttttattggc tcagatggag ttaaagaagt
    27181 attagttaca tcatcaacta tacctactgt aaatccattt ttaggttgtt ctgaaactaa
    27241 gttatcaaaa acagttttta tatctgaagg agtagtatct tttgaaccta atccgtatct
    27301 tccacctatt atcataggag cattttcttt tccatagaat acatcacgta catctaagta
    27361 taatggttca ccagttgaac ctggctcttt agttctatca agtacacata ttctttcaac
    27421 agtagatggc atagcatcta agaaatgttt cattgagaat ggtctatata aatgaacttt
    27481 aactaaacca tatttttctc ccttagcatt taagtaatct atagtttctt ctattgtttc
    27541 tgttactgaa cccatagcta ttaatacgta tttagcatct tctgctccat agtaatcaaa
    27601 taaactatgt tttctacctg ttaaattact cattttttcc atgtattttt caactatacc
    27661 tactatattt tgatagtatt tattagaagc ttctctagtt tggaaatata tatctgggtt
    27721 ttgagcagta ccacgagcaa ctggatgatt tggagataaa gcattatctc taaatgcttg
    27781 aactgcttca aagtttaata aacttgcata atcttcattt tctagtaatt caactttttg
    27841 gatttcatgt gaagttctga atccatcaaa gaaatgtata aatggtaatc taccttctat
    27901 tgcagctaaa tgtgcaacag gtgctatatc agctacttct tgaactgaac cagaagctaa
    27961 taatacacat ccagtttgtc tagcagccat aacgtcttgg tgatccccaa atattgataa
    28021 cgcttgagat gctaaagcac gagcacttac atggaatact cctggtaata actctcctgc
    28081 aactttatac atgttaggta tcattaataa taaaccttga gatgctgtaa aagtagaagt
    28141 taaagctcct ccttgtaaag aaccgtggaa tgttcctgat gcacctgctt ctgactgcat
    28201 ttcaacaaca ttaactgttt gtccaaatat attttttctt ccttgtgatg cccactcatc
    28261 aactacttca gccatagttg aagatggtgt gattggatat atagctgcta catctgtaaa
    28321 cgcataggca acatgagctg cagctgtatt tccatcaagt gttttcataa acttagccat
    28381 aatatgtatc ctccttgata gctacaattg tacttgttta taacaagtct atttttttat
    28441 atttacatat aatttacaca acaaaactat aaacacacat atagtcgcgc aaatactcta
    28501 tcttctaata taatactaca gattagataa aattcctttg aaaattgtaa attaattaca
    28561 tatttaagta gatttttttt aaaattaaat atgttaagtt tttttcataa ttttgtatac
    28621 aatatataat aaattaagtt agatttttca aactttttta tctatttgtt catattagta
//
LOCUS       DAMJVK010000047        28051 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.047, whole genome shotgun sequence.
ACCESSION   DAMJVK010000047 DAMJVK010000000
VERSION     DAMJVK010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28051
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.047"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(53..568)
                     /locus_tag="PC414_002108"
     CDS             complement(53..568)
                     /locus_tag="PC414_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HDF2464503.1"
                     /translation="MDFLAKYKVLKKQNSSKSCLVCGTQNELGLKADFYELENGELVS
                     ICNTKDWHQSYPGRVHGGMSAAILDETIGRAVSINDDQIWGVTVSLELKYKKPVPTDA
                     TIKVVGRITKENRKLFEGTGEIILPNGDIAVTATGKYMKMPIGQIAEGDFSNEEWFFE
                     ESKEKVEYIEL"
     gene            complement(931..2001)
                     /gene="leuB"
                     /locus_tag="PC414_002109"
     CDS             complement(931..2001)
                     /gene="leuB"
                     /locus_tag="PC414_002109"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HDF2464504.1"
                     /translation="MNCNIAVIKGDGVGPEIIDEGIKVLNKICCKFNHRFDCEYVLAG
                     GCAIDETGEPLPNKTVETCRKNEAVLLGAVGGPKWDKCKGDKRPESGLLKLRESLGLF
                     ANLRPATMYESIKEASPLRTDIVEKGIDFVVVRELTGGIYFGERGRKIIDGIENAYDV
                     EIYNENEIRRIGKRAFEIARNRNKKLISVDKANVLESSRLWRSIMEDLAKEFEDVELS
                     HMYVDNAAMQVVKDPSQFDVIVTNNIFGDIISDEASMITGSIGMLPSASLREDSFGMY
                     EPIHGSAPDIAGKDIVNPIATILSVSMMLRHSFNLEEEAKCIEDAVQSVLNKGYRTID
                     IYNGVGNVVGTREMGELIVNEI"
     gene            complement(2008..2499)
                     /gene="leuD"
                     /locus_tag="PC414_002110"
     CDS             complement(2008..2499)
                     /gene="leuD"
                     /locus_tag="PC414_002110"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HDF2464505.1"
                     /translation="MIANGSVFKFGDNIDTDVIIPARYLNIADYKELATHCMEDIDDK
                     FISKVKKGDIIVATKNFGCGSSREHAPIVIKESGVSCVIASTFARIFFRNSINIGLPI
                     LECEEAANNIDEGDNIEVDFSTGVIKNITKGKEYKAEPFPEFMQNIILNEGLINSIKA
                     NRG"
     gene            complement(2514..3791)
                     /gene="leuC"
                     /locus_tag="PC414_002111"
     CDS             complement(2514..3791)
                     /gene="leuC"
                     /locus_tag="PC414_002111"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HDF2464506.1"
                     /translation="MGMTMTQKILAKHAKLNEVKKGQLIEADLDVVLGNDITSPVAIR
                     EFEKLGIEDVYDKTKVVMVLDHFTPNKDIKSAEQCKFTRSFAKSKGVVNFFDVGDMGI
                     EHVLLPEKGIVTAGDVIIGADSHTCTYGALGAFSTGVGSTDMGAGMATGKCWFKVPGA
                     IKFVLKNKPNKWISGKDIILHIIGEIGVDGALYKSMEFCGDGVEYLSMDDRFTICNMA
                     IEAGAKNGIFPVDDKTMEYINSHKCSTMTKDVNIYEADEDAVYDEVYEIDLAELKETV
                     AFPHLPENTRTVDEIDKDIKIDQVVIGSCTNGRISDLEVVAEIMKGKKVADGVRVMIF
                     PGTQKVYLEAIEKGYITTFIEAGAAVSTPTCGPCLGGHMGILAAGEKSISTTNRNFVG
                     RMGHVDSEVYLASPAVAAASAITGKISKPSEII"
     gene            complement(3748..5490)
                     /gene="leuA"
                     /locus_tag="PC414_002112"
     CDS             complement(3748..5490)
                     /gene="leuA"
                     /locus_tag="PC414_002112"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HDF2464507.1"
                     /translation="MKCGKYKKYDKMQIVNRKWPDNEIFKAPIWCSVDLRDGNQSLPT
                     PMSVNEKVRMFKMLIDTGFKEIEVGFPSASNTEYTFLRKLIDENMIPDDVTIQVLTQS
                     RAHLIEKTFESIRGCKKAIIHLYNSTSVLQRDVVFNMSKQEIIDIAVEGAKLFNEEVK
                     KYPETEFTFEYSPESFTGTEMDYALEICEAVIDVWKPTTQKKVIINLPSTVEMATPNV
                     YADQIEWFCKNISCRDSIILSLHTHNDRGTCTAASELGLLAGADRLEGTLFGNGERTG
                     NMDIVNVGLNLYTQGIDPELDFSNIDKIIGIYEDCTKLMVHDRHPYAGNLVHCAFSGS
                     HQDAIRKGMIAMKNRDNDYWEVPYLPIDPHDIGREYKEIIRINSQSGKGGAVYIMETD
                     YGFMIPKNMHSDFGNVVKMESDRIGEELSSEAIFNLFKKEYIEVESPYKVKKYKIKSM
                     DELNYENDDSNDTNMIEMTARISYMGNEQRIVGIGNGPVDSFNNALKQCGMKDYKFRY
                     YWEHALEEGSHSRGVAYVGIEHNNEVYFGVSISENINTAAINALMNAINKSYIEEEIK
                     NGDDYDAENISQTC"
     misc_binding    complement(5614..5860)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(6008..6454)
                     /locus_tag="PC414_002113"
     CDS             complement(6008..6454)
                     /locus_tag="PC414_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464508.1"
                     /translation="MSKNKFIYMNYYDNGYFKKGMTIYMVLALIILATIFIYSTTILY
                     ILCIITAVFLFKQGRDFSNKYEGKVYITLDNHSILINNQCIFSIKSKQSQKFNYKIIE
                     KIEVVKNILNIYTDESNYKIRLRALSLEDEKKLLNIIDEKMKKFKA"
     gene            complement(6475..7194)
                     /locus_tag="PC414_002114"
     CDS             complement(6475..7194)
                     /locus_tag="PC414_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HDF2464509.1"
                     /translation="MKKSIMDVHCHTLISGHAHSTFKENVEEASKKNIKYLGISDHGP
                     NMPGGPHPFYFYNLHLLPREVQGVKILRGIEGNIMDYNGNLDVQEDMLQHLDYIIASL
                     HRPCIASGTKEENTNAILKVMDKPKVKIIGHPDDSRYPLDYEPIVKKAKDKNILLEIN
                     NSSLSSNSHRTGTWENVSHMLTLCKTYGARVILGTDSHICYSIGEFENAEKVLEAVDF
                     PDELVINYHEDEIIEFFDINF"
     gene            complement(7312..8586)
                     /locus_tag="PC414_002115"
     CDS             complement(7312..8586)
                     /locus_tag="PC414_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HDF2464510.1"
                     /translation="MFIMENDLTMQELLDQQEQVFSKVKVGELTTGKITAVRNDEVQL
                     GLDYGFDGIIPISELNIEKNQYIEDIYHIGDEITAVITKVSQKDGTITLSKLQLDKRN
                     DFAELQKAYDEHRIITVNVEKNIDKGVFANYNTYTFFIPISQLDTKFITDTSKFVGLN
                     LEVYIKELDVRKNRLVASHRDVLQERINKEREERRAQIKAEKEAERARIKQEREEEKA
                     RIKAAKEDLFNSLEVGQKRDGKVTKIMPYGAFVDIGGIEGLAHINNLAWTRVESVEDV
                     VSEGQEVEVYVLDVNKETKKIALALKDINNDPWDLIAKEVQIDDVVNVKVLRIIEKGA
                     FVQIKEGVDAYLPISELSDTRVAKVTNVVNIGDEVKVKILDFKPKTKRMLVSIKEATR
                     EPEEDITEYLEVEESLGSIGELFKDKFKDLEV"
     gene            complement(8786..10153)
                     /locus_tag="PC414_002116"
     CDS             complement(8786..10153)
                     /locus_tag="PC414_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2464511.1"
                     /translation="MENSNTNLGSESVGKLLFKLATPAIIAQIVNVLYNIVDRIFIGR
                     MENGEVAMAGVGVAFPIIIIITACSYLIGMGGGPLAAIKMGEQNNDEAEKIMSNSFSV
                     LVILAILLTIGFKIGKEPLLWMFGASESTIGYSMDYLNIYLIGTVFVQISMGMNTFIN
                     TQGFATTGMMTVAIGALINIILDPIFIFGFNMGVKGAALATIIAQGVSAIWVLMFLFG
                     KKSILKIKKKYMIPKASIILPVLGLGISPFIMQSTESLVLIALNSKLQMYGGDLAVGS
                     MAIMSSIMQILMLPNMGVTQGAQPIISYNYGSGQLDRVKKTFKLCLLSCFTYSTILWL
                     LLMIFPAFFVSIFNKNPQLLSMTSWSIKIYFAGAFMFGIQIACQQTFLALGKATISLV
                     LALLRKIVLLIPLIFILPTFFNEKLFAVILAEPVADITAATITAISFFIFYKCFLSKP
                     KAIKE"
     gene            complement(10403..10867)
                     /locus_tag="PC414_002117"
     CDS             complement(10403..10867)
                     /locus_tag="PC414_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HDF2464512.1"
                     /translation="MSLRIGGNSLGYLHILILSMVPVTELRGAIPIGIAMGLNPIWVY
                     VFSVIGSTLVSIPLILTFRHVLQFLRGKKLFKGIADVIDRKINSRMKKLKSVSIIGII
                     LFVGIPLPTTGTWTASAIASILKMRIKDAFMGVFLGNLLSGVIVSALSLHII"
     gene            complement(10872..11501)
                     /locus_tag="PC414_002118"
     CDS             complement(10872..11501)
                     /locus_tag="PC414_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HDF2464513.1"
                     /translation="MKKSVLLLDVDYTVINTDSMIDFFIYSLKNKTFKTIIKLPYIIF
                     ILFMYLIRMIPLKKAKEAIFYPIVDFSEEDLKKFFDDCIMKKINESMKKVIYKNKEED
                     NVIIMITASPYAYMKYFKYYGLADEVIGTEFFYENSRYKNQFIGENCKGIEKVKRIKA
                     VLGKLGIEIDYENSYAYSDSKSDLPMLSLTKNAFLVSKKDGEVIEQINS"
     gene            complement(11535..12422)
                     /locus_tag="PC414_002119"
     CDS             complement(11535..12422)
                     /locus_tag="PC414_002119"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HDF2464514.1"
                     /translation="MKQTNTSIRLLTKISDYIKLMRIKQYIKNFFVFSAIIFSNNILN
                     INLFLNTFIAFVCFCLMSSSVYALNDAIDMDKDKKHPKKCNRPVASGRISKKSANILF
                     VVLALISVCLSTVVSFNLSIILSLYLINNILYSLKLKNIILLDVFSISLGFILRVYAG
                     CVAISVSLSNWIILCTFFLSLYLALGKRKKEIETLRDDAVEHRKILEDYDIENLNQMM
                     IVILSSTIVCYALYSTSNPEKPHMIFTTIFVVYGVLRYNYIINTTNENNPTDIVLKDN
                     ALKINVILWIITCLIILIF"
     gene            complement(12451..13689)
                     /locus_tag="PC414_002120"
     CDS             complement(12451..13689)
                     /locus_tag="PC414_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464515.1"
                     /translation="MDGDILMSKILKYKNEMFLFILVVITYLIITKIFFSKTTIFYDL
                     NNTYDVLLDTDTGVLFNLNVFAISQDNSKHILFSAIISIFAYPIYLFCTSIANPGTTD
                     FNSAYGFGLICLQIITSAMSITLVFNHIKKIKMQRLTLILLTMIMIFSFPQLFMTLNV
                     ERFIYSQFSLIFFIVIANKMKGKNSYLVELAAIPLFGITISNIYLYFFNMIFEFKLKI
                     GKMLKHLITFILMAYICVVSTKSYESFMNLGNVIQYDTKFISGEPILEKIAMIIERLL
                     YSVFYFPGASIKKGLFLQNGEVATIPVILTLLALCFCVLSVIENSEKRVPKLCMGIII
                     FNLTLHGIVGYNLVNSSIMAINFSFAVIILLAYFTKALRKNEKNMYNIFLSLLLVTIV
                     ISNVNGFIEILNIGIKSYPV"
     gene            complement(14073..15215)
                     /locus_tag="PC414_002121"
     CDS             complement(14073..15215)
                     /locus_tag="PC414_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDF2464516.1"
                     /translation="MRYVEYGKTGKMVSVVGYGGLRFDLEKSNQENADLVKYAYEKGI
                     NYFDTAPGYCDDRSEDIFGLAFRQMIKEGKTDFYVSTKEKPKACDTSEKAIEAVKKSI
                     ERLGVSKINFYHVWNIRKMEHYELAMRPGGQYEGLLKCKEEGLIDHIVFSSHQPGDEV
                     IDILSENKFEGVTMGINILNFPYRLKGAKYAVDNGYGVVAMNPLSGGTIPKYNKELSF
                     LSKDGESTIESALRFNIGISQISISLIGFNKKQDIDDACKIADENRIYSDEDIHDIET
                     RLNKNMNEICTGCGYCKVCPKGINTPAYMLFYNEKQMFKKSDEEMTKLVYGLGHWNYT
                     MNSKAKAKECISCGKCEVECTQHLPIIDRLKEIKKWEEDGANSVKV"
     gene            complement(15356..16222)
                     /locus_tag="PC414_002122"
     CDS             complement(15356..16222)
                     /locus_tag="PC414_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDF2464517.1"
                     /translation="MDNYKKVKQVLWIILFANFAVALLKIIIGNQIKSYSMTADGFHS
                     LSDGASNIVGLIGIFFASKPKDKNHPYGHKKFEIITSLFISGMLFVIAIKIILSAVLR
                     IANPVVPAITIESLIALIITLFINIFVCMYEYRIGTKLNSYVLISDSLHTRSDIFVSL
                     GVLVTLVGVKLGFPVIIESIVPIIISAFIIYSAYGIFRPSIGILVDRVAVDEDYIKEI
                     VFEFNEVRDVHNIRSRGSKSSIYIDMHVMVDPFISVEQSHDLTHKIEKQIQEEINENA
                     QVIVHIEPFYSF"
     gene            complement(16557..18104)
                     /locus_tag="PC414_002123"
     CDS             complement(16557..18104)
                     /locus_tag="PC414_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDF2464518.1"
                     /translation="MANFFNFILLQKDKIIELLIQHMSLTVTSILIAIIVGVPLGIII
                     SRISSLRKFVLGFVNLVQAVPSMALLGLLVPILGIGSKPAIFMVVVYSLLPIVKNTYI
                     GITSIDPVVLESAKGIGLTRNQTLFKIQFPLALPIIMGGVRISAVTAVGLMTLAAFIG
                     AGGLGYLVFSGVQTVNNNMILAGAIPSCIIALLVDYLFSKIEVAVTPKGLNPKAPKKN
                     YIALKVISVVVVVSMLFVVFSSSFSSKKDTITIGSKDYTEQLILGNVYAELVEKNTNL
                     KVKKNLNLGGSSVAFNAIKSGELDMYVDYTGTLLVNVMKHAPIKDADEAYNVVKDTME
                     KENQLTLLDPLGFNNTYTLAMMPETAEKYGINTISDLTKYGKEFTFSPTLEFENREDG
                     LVGLSRDYGLKFKDVKAMNGSLRYTALDNNESQVIDAFLTDGLIKKFNLKILEDDKNF
                     FVPYYAAPLVREDTLKKYPELEKVMNMLSGKINEETMRELNYQVDELGKSPEEVAHSF
                     LVKEGLV"
     gene            complement(18097..19233)
                     /locus_tag="PC414_002124"
     CDS             complement(18097..19233)
                     /locus_tag="PC414_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2464519.1"
                     /translation="MIEIRNVTKKIGNNVILDDISLVVETGTLVVLIGSSGCGKTTTL
                     KLINKLIKPTSGEIYINGKPISQENEIELRRKIGYVIQNTGLFPHLTIKENIELIPRL
                     KKEKSVEEIEKRTLQLLEMVGLDSDEFLNKYPSELSGGQQQRIGVARAIATDAEIILM
                     DEPFSALDPITRTSLQEQLFSLQDELKKTIIFVTHDMDEALKIADKICIMKDGRIAQY
                     DTPENILRKPANDFVKDFIGEDRVWDNPEYIKARDIMIKNPIAVNSTRTVTQGIEIMR
                     TSKVDSLLIIDRAKTLKGIVTVKDMKDIDDKSILLADIMSSEPLHVNEGDNLVEILNV
                     MNRNSVGYIPVISDENKLVGLITRSSLLSVLSEQFLEMEVSVLG"
     gene            complement(19496..20593)
                     /gene="dinB"
                     /locus_tag="PC414_002125"
     CDS             complement(19496..20593)
                     /gene="dinB"
                     /locus_tag="PC414_002125"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDF2464520.1"
                     /translation="MKHYRKIIHIDMDAFYASIEQRDNKKLKGRPVIVGGNPQSRGVV
                     ATCSYEARKFGIHSAMPSAVAYNRCPYAVFVRPRFDVYKSVSEQIRDIFYRYTDLVEP
                     LSLDEAYLDVTKNKKNIDSSIEIAKQIKKDIFREVGLTSSAGVSYNKFLAKIASDLRK
                     PNGLTVITEENAQDFLDKLPVNKFFGVGKVTSNTLKNLGIKTGYDLRCLNLFELENIF
                     KKRGYELYKFARGIDDRPVEPNRVRKSVGAETTLSHNLDIDEEETRNILDELCEEVCH
                     RLKNSEKFGKTLTLKIKYEDFTKITRSLSLEHYIDEYNDIRSGVDNLLRNVEVNGKQI
                     RLLGVTISNLSDKKETYKDITLFEYMDSIQM"
     gene            complement(20983..22488)
                     /locus_tag="PC414_002126"
     CDS             complement(20983..22488)
                     /locus_tag="PC414_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HDF2464521.1"
                     /translation="MKVGFDHKKYLEEQSKYILERVNNFDKLYLEFGGKLMGDLHAKR
                     VLPGFDENAKIKVLQHMKEKVEVVICVYAGDIERNKIRGDFGITYDMEVLRLIDDLRG
                     YELDVNSVVITRFEGQPATTVFINKLERRGIKVYKHYPTKGYPSDVETIVSDEGYGAN
                     EYIETTKPIVVVTAPGPNSGKLGTCLSQLYHENKRGNVVGYSKFETFPVWNVPVKHPL
                     NIAYEAATVDLKDVNMIDSFHLETYGEMSVNYNRDLELFPVLKKIIEKITGKESIFQS
                     PTDMGVNRVGFGIIDDEVIRKASIEEIIRRYFKTACEYKKGQVDKGAYDRMKMIMEEL
                     NLKPEDRNVVLPARNYSNKLKESADKNDTCPVVALELEDGTILTGKGSDLMNGTAAVV
                     LNAIKHLANISDDMHLISPVILEPIINLKTKTLASRNVSLSCQEVLTALSICAVTNPT
                     AQFAMEKLALLKGCQAHSTTILNRDDEQTFRKLGIDVTCDPEYPSQNLYYS"
     gene            complement(22749..23099)
                     /locus_tag="PC414_002127"
     CDS             complement(22749..23099)
                     /locus_tag="PC414_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464522.1"
                     /translation="MAIVDKIFNEIKLMVARLLLGKKYSEYEEKDLVYNTEEEVILIT
                     LKRLVFQGNVNEAEEILFDKAKSVNSENMQYIAIEFYTMLMEKTDEELEAMNFSKQEV
                     YQGIEDIRKVLNLE"
     gene            complement(23322..23513)
                     /locus_tag="PC414_002128"
     CDS             complement(23322..23513)
                     /locus_tag="PC414_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464523.1"
                     /translation="MLDRFFGEGGFGGCAWWIIILFFLFLAFQDCWAEMDLCAWIPFL
                     ILLLILCECGDVFDGGCGC"
     gene            complement(23631..24557)
                     /locus_tag="PC414_002129"
     CDS             complement(23631..24557)
                     /locus_tag="PC414_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2464524.1"
                     /translation="MRKIVLKDILFLVTTLSIIVILLLVTNITTNSKIKSTSESYLKS
                     DKNSVRQNGIQRSTKSSTKNTAFKTTILNTGKSDCILVEIGNKVIMIDTGEDKNGKQI
                     VDRLKEKGINTLDYLILTHLDKDHIGGVDSVLSSVKVKNIIQANYKKDSKQYDEYIDS
                     LKKADIEPVLLKDNMNIVINGAEINIHPASKSKYESSNDYSIITNISYGAHKFLFAGD
                     AEEERLSEFINGNTLKYDFVKMPHHGRYDKLTETFLESISPQYAVITCSEKKEPEEGV
                     LKILERLNIKTFLTSNGEVVINSDGKTLSVNQ"
     gene            complement(24691..26187)
                     /locus_tag="PC414_002130"
     CDS             complement(24691..26187)
                     /locus_tag="PC414_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF2464525.1"
                     /translation="MFEFETQLKRLKHEVLTAVARLSIEENLTKENIEKIPYEIIKGE
                     KARYRCCVYKEREVVLERAQLALSLTPNSKYGDINPDSVSLDTDEQIIYIVEAACDRC
                     PINEFTVTELCRGCLAHRCKEACKFGAISYINGRAYINHDLCKACGMCKSSCQYDAIS
                     EVVRPCKSVCPTGALDFNRNTMKAMIHEDNCINCGACMSACPFGAISDKSLIAPVAKK
                     LAKKENMYAIVAPAITGQIDSNITYGQLKNAIKSLGFVDMIEASCGADAVTVHESNEL
                     IERIKSGDNYMTNSCCPGFFKYIEKMFPDEKDRISSTVSPMVATGRLVKKIDSDAKVV
                     FIGPCTAKKSEATEKSLKDSVDYVLTFEELMALFEAFNIDLSKCSNEDINDGSIFGRG
                     FGASGGLAKSVQNYIAEKGLSFDFEPIKVSGPRQIKKTMTMAKLGRLEGNFIEGMMCE
                     EGCINGAGKFLSGVKAKNTFDRKNGQSTKKSVLSNDKIKEFEDVNLER"
     gene            complement(26467..27957)
                     /locus_tag="PC414_002131"
     CDS             complement(26467..27957)
                     /locus_tag="PC414_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF2464526.1"
                     /translation="MFQFETQIHKLRHDVLTAIARLGKEDNLTREAMDNIQYEIIKGE
                     KPTYRCCVYKERAIISERVNVSMGLQPGQDKIDLDTMDIEEDEQIIYILEAACDSCPI
                     NEFTVTDICRGCLAHRCKEACKFGAITHVGGMAYINHELCKACGMCKKACQYDAISEV
                     VRPCKSVCPTNALGFDRENMKAMIHEDKCLNCGACMSACPFGAISDKSLIAPVARKLV
                     QKEKMYAVVAPAITGQVEANVTYGQIKNAIKSLGFVDVLEAACGADAVTVHESLEFAE
                     RMEKGEAFMTNSCCPGFVGYIEKTYPNHVGKISSTVSPMIASGRFIKSHEPDAKVVFI
                     GPCTAKKSEAAKKELKGAVDYVLTFEEIMALFEAFDIDLAKCSNEDIDDASIFGRGFG
                     AAGGLTKAIENYLAEKGIGVNFEPVKISGSREIKKTMTMANLGKLEGNFIEGMMCEGG
                     CINGSGKFLSGAKVKATFDRKNSQSTKKSVLANTKIKDFSNINLEK"
BASE COUNT         9149 a         5146 c         2884 g        10872 t
ORIGIN      
        1 tattttttct aaatataaaa tttatgtttt aaaatataaa atttttattt aatcaaagtt
       61 caatatattc cactttttct ttcgattctt caaaaaacca ttcttcatta gaaaaatcac
      121 cttctgcaat ttgtccaata ggcattttca tatactttcc agttgctgtc actgctatat
      181 ctccatttgg tagaattatt tctcctgtac cttcaaataa ttttctgttt tctttagtta
      241 ttcttccaac aacttttata gtagcatctg ttggtactgg tttcttatat ttcaactcta
      301 aagatactgt tactccccat atttggtcat catttatact tacagctctt ccaatagtct
      361 catccaatat tgcagcagac atacctccat gaactctccc aggataactt tgatgccaat
      421 cctttgtatt acaaattgat acaagctctc cattttcaag ttcataaaaa tctgctttaa
      481 gtcctagctc attttgtgtt ccacaaacta aacaagattt actactattt tgcttcttca
      541 aaactttata ctttgccaaa aaatccatct cctaaccgac attagtagtc gcttaatatt
      601 aaataattct aaaatatatt atataatatt aataaaatcc ctgcaaactt ctaataataa
      661 aaataggcta cttcaatatg ataagtttaa tttaaagtat tattttccaa aaaataactt
      721 tatcaaaata gaaactcaaa gtagatataa acctcttttt cgttattaaa atagtaaaaa
      781 tatatttgac ttaatataaa aaagagtgtc tcattaacta ataaagttta ttttgagaca
      841 cccctcataa aacaaattat actacaatag atttttacta attctacaag ttatatattt
      901 aaattatatt attcaaatta aactataatt ctaaatttca tttactatta attcgcccat
      961 ttcccttgtt cctacaacat ttcccacacc attatagata tcaatagttc tataaccttt
     1021 attgagtaca ctttgtacag catcttcaat acacttagct tcttcttcta agttaaaact
     1081 atgtcttaac atcatgctta cagatagaat agtcgcaatt ggatttacaa tatcttttcc
     1141 agctatatca ggtgcacttc catgtattgg ttcatacata ccaaaactat cttctctaag
     1201 tgatgctgat ggtaacatac ctatagaacc cgtaatcata gatgcttcat cagatattat
     1261 atctccaaat atattattag tcactattac atcaaattgg ctagggtctt ttacaacttg
     1321 catagcagca ttatcaacat acatatgtga cagttctaca tcttcaaact ctttagctaa
     1381 gtcttccatt atacttctcc aaagtcttga actctcaagc acatttgcct tatctacact
     1441 tataagtttt ttatttctat ttctggctat ttcaaaagct ctcttaccaa ttcttcttat
     1501 ttcattttcg ttatatattt ctacatcata agcattttcg ataccatcaa tgatttttct
     1561 tccacgttct ccaaaataaa tgccccctgt taattccctt acaacaacaa aatcaatgcc
     1621 tttctcaact atatccgttc taagtggtga agcttctttt atactttcat acattgtagc
     1681 tggtcttaaa tttgcaaata aacccaatga ctctcttaat tttaaaagac cagactctgg
     1741 tcttttatct cccttacatt tatcccactt tggtcctcca acagcaccaa gcaatacagc
     1801 ttcgtttttt cgacaagttt caacagtttt attaggtaga ggttctccag tttcatcaat
     1861 cgcgcatcct ccagctaata catattcaca atcgaatcta tgattaaatt tacaacaaat
     1921 tttatttaga acctttatac cttcatctat aatttccggt ccaacaccat caccttttat
     1981 gactgctata ttacaattca tacttcacta ccccctattt gcttttattg agttaattaa
     2041 gccttcattt aatataatat tttgcataaa ctcaggaaat ggttctgctt tatattcttt
     2101 tccttttgtt atatttttta tcacacctgt agaaaaatct acttctatat tatccccttc
     2161 atctatgtta ttggcagctt cttcacattc aagtatagga agccctatat ttatagagtt
     2221 tctaaaaaat attctagcaa aagtagaagc aatcacacag gatactccac tttcttttat
     2281 tacaattggt gcatgttctc ttgatgaacc acaaccaaag tttttagttg caactataat
     2341 atctcctttt tttaccttag aaataaattt atcatcaata tcctccatac aatgcgttgc
     2401 tagttcttta taatcagcta tatttaaata tctagctggt attattacat ctgtatctat
     2461 attgtcaccg aatttaaaaa cacttccatt ggcaatcata aattttacct cccttaaatt
     2521 atttctgatg gttttgatat ttttccagtt atagctgatg ctgctgctac agctgggctt
     2581 gcaagataca cctctgaatc aacatgcccc attcttccta cgaaattcct atttgttgtt
     2641 gaaatggatt tttctcctgc tgcaagtata cccatatgtc cccctagaca tggaccacaa
     2701 gttggtgtag aaactgctgc tcctgcctct ataaaagtgg taatataccc tttttctata
     2761 gcttcaagat atactttttg agttccagga aatatcataa ctctaactcc atctgctact
     2821 tttttgcctt tcattatctc agctactact tcaaggtctg atattcttcc atttgtacaa
     2881 gagcctatta caacttggtc tatctttata tccttatcta tctcatctac tgttctagta
     2941 ttttcaggaa gatgaggaaa ggctactgtt tcttttagtt cagctaaatc tatttcgtat
     3001 acttcatcat agactgcatc ttcatctgct tcatatatat ttacatcttt tgtcatagtt
     3061 gaacacttat gtgaatttat atattccata gtcttatcgt ctactggaaa aattccattt
     3121 tttgctcctg cttctattgc catattacaa atagtaaatc tatcatccat agataagtat
     3181 tctactccat ctccacaaaa ttccattgac ttgtaaagtg ctccatcaac acctatctcc
     3241 cctattatat gaagtattat atctttacca cttatccatt tatttggttt attcttcaaa
     3301 acaaatttta ttgctccagg aaccttaaac caacattttc ctgtagccat acctgctccc
     3361 atatcagttg aacctacacc agtagaaaat gctcctaatg ctccatatgt gcaagtatga
     3421 gagtcagcac ctataattac atctccagca gttactatac ctttttcagg aagaagaaca
     3481 tgctctattc ccatatctcc tacatcaaaa aaatttacaa ctccttttga ttttgcgaaa
     3541 cttcttgtaa atttacattg ttctgcactc tttatatctt tattaggtgt aaagtggtct
     3601 aaaaccatca ctactttagt cttatcataa acatcttcaa ttcctaattt ttcaaattct
     3661 ctaatggcta ctggtgatgt tatatcatta cctaaaacta catctaaatc tgcttcgatt
     3721 aattgccctt tcttaacttc atttaattta gcatgtttgg ctaatatttt ctgcgtcata
     3781 gtcatcccca ttttttattt cctcctcaat ataactttta tttattgcat tcataagtgc
     3841 attaatagct gctgtattta tgttttccga tatacttaca ccaaaataga cttcattatt
     3901 gtgctctatt cctacataag ctacccctct tgaatgtgac ccttcctcta atgcatgttc
     3961 ccagtaatat ctaaatttat aatctttcat gccacattgc tttagtgcat tattaaatga
     4021 atctacaggt ccatttccta taccaacaat cctttgttca tttcccatat aacttattct
     4081 tgcagtcatc tcaatcatgt tagtatcatt agaatcatca ttttcatagt ttaattcatc
     4141 cattgatttt attttatact tcttaacttt ataagggctc tcaacttcaa tatattcttt
     4201 cttaaacaaa ttaaatattg cttcactact aagttcttcc ccaattctat cagattccat
     4261 tttgactaca tttccaaaat cagaatgcat attcttaggt atcataaatc cataatctgt
     4321 ttccattata taaacagctc ctccttttcc agattggcta tttatcctta taatttcttt
     4381 gtattctctt ccaatatcat gagggtctat tggtaaatat ggaacttccc agtaatcatt
     4441 atctctgttt ttcattgcta tcatgccttt tctaatagca tcttgatgtg aacctgaaaa
     4501 agcacaatgt acaagattac ctgcatatgg atgtctatca tgtaccataa gttttgtaca
     4561 atcttcatat atacctatta tcttatctat attgctaaaa tctagctctg ggtctattcc
     4621 ttgtgtataa agattaagtc ccacatttac tatatccata ttcccagttc tttcaccatt
     4681 tccaaatagt gttccttcaa gtctatcggc accagccaat aatccaagtt cacttgcagc
     4741 cgtacaagtt cctctatcat tgtgagtatg caaacttagt ataatagaat ccctacaact
     4801 tatattttta caaaaccatt ctatttggtc tgcatatacg tttggtgttg ccatttcaac
     4861 tgttgatgga agatttatta ttactttttt ttgagtagtt ggtttccaaa catctatcac
     4921 tgcctcacat atttcaagtg cataatccat ctctgttcct gtaaaactct ctggtgaata
     4981 ttcaaaagta aattctgttt ctggatattt ttttacttct tcattaaata attttgctcc
     5041 ttcaactgct atatctatta tttcttgttt actcatatta aaaaccacat ctctttgaag
     5101 aacagatgta gagttatata aatgtataat tgctttttta catcccctta ttgattcaaa
     5161 tgttttttct ataagatgag ccctagattg tgtaagtact tgtatagtta catcatctgg
     5221 aatcatattc tcatctatta attttcttaa aaatgtatac tctgtatttg atgctgatgg
     5281 aaatcctacc tctatttctt taaatcctgt atcaataagc attttgaaca ttctgacttt
     5341 ttcatttaca ctcataggtg ttggcagtga ctgatttccg tctcttaaat cgacactaca
     5401 ccaaattgga gccttaaata tttcattatc tggccattta cgatttacta tttgcatttt
     5461 atcatatttt ttatactttc cacacttcat gttatttccc cctttttctg ttttttgtat
     5521 tcagtatacc tcatatcgac gcaatcctgc acaattcata aggataaatg tcaattttat
     5581 cattagaata aaaaaaacct catctctaat aaaattagag acgaggtttt gctcgcggta
     5641 ccactctatt tagctttcta aaaagctccc ttagtaatag ccaatacaat aattataatg
     5701 actatatttc ctataacgtg gaattactcc gattattcta agatttaaat cttagaaaac
     5761 agctcctaga caagttcaga actatcaact actaaatcac accatccttt agctctctga
     5821 aagtttcatt gttcttacta cttctattca tcactttttt aatcatttaa attttctaaa
     5881 tattttgatt taatagttat tatatacaca ttaaaatgta ttgtcaacac aaaaaataat
     5941 tatctcaatt ttttattttt gataagcaat tctataaaac atgctaacta ttgaaaacag
     6001 tatgatatta tgccttaaac tttttcatct tttcatctat gatattcaat aatttttttt
     6061 catcttccaa actcaatgcc cttaatctaa ttttataatt agattcgtca gtgtatatat
     6121 ttaatatatt ttttactact tctatttttt ctattatctt ataattaaac ttttgacttt
     6181 gtttagattt tatactgaat atacattgat tatttattaa tattgaatga ttatcgagag
     6241 taatatatac ttttccctca tatttatttg aaaaatcacg accttgttta aatagaaaaa
     6301 ctgctgttat tatacataaa atatataata ttgtcgttga atatataaaa attgtagcta
     6361 aaattataag tgctaaaacc atatatatag tcattccttt tttaaaataa ccattgtcat
     6421 aataattcat atagataaac ttatttttac tcaaattttt tcacttccaa gattctaaaa
     6481 atttatatca aaaaattcta taatttcatc ttcatgataa tttatgacta attcatctgg
     6541 aaaatctact gcttctaaaa ctttttctgc attttcaaat tctcctatag aataacatat
     6601 atgagagtca gtaccaagaa ttactcttgc accatatgtt ttacacaatg tcaacatatg
     6661 acttacattt tcccaagtac ccgttctatg agagtttgaa cttaatgatg aattatttat
     6721 ttctaaaagg atatttttat ctttagcttt ttttactata ggttcataat ctaaaggata
     6781 cctactgtca tctggatgtc caattatttt taccttaggt ttatccataa cttttaaaat
     6841 agcatttgta ttttcttctt ttgtaccaga agctatacaa ggtctatgca aacttgcgat
     6901 tatataatcc aaatgttgca acatatcttc ttgaacatct aaattaccat tataatccat
     6961 tatatttcct tctattcctc taagaatctt tacgccttgc acttcacgtg gaagcaaatg
     7021 caaattataa aaatagaatg gatgtggtcc tcctggcata ttaggtccat ggtctgatat
     7081 tcctaagtat tttatattct tcttactcgc ctcttcgaca ttttccttga aagtactatg
     7141 tgcatgtcca cttattagag tgtgacaatg tacatccata atagactttt tcattaaatt
     7201 tcctcctaat attttacata aattcgtgaa aatagctaaa gggctatttc aaaactaaag
     7261 ctgtcataat agacagtgta acgttttaaa acagccccat tttcaattat tttacacttc
     7321 taaatcttta aatttatctt taaataattc tccaatactt cctaaagatt cctctacttc
     7381 taggtattct gttatatcct cttctggctc tctagttgct tcttttattg aaaccaacat
     7441 tctcttagtc tttggtttaa aatcaagaat cttaacttta acctcatctc caatattaac
     7501 aacattagta actttagcca ctctagtatc actcaattca gatattggta aatacgcatc
     7561 tacaccttct ttaatttgaa caaaagcccc tttttctatt attctaagaa ctttaacatt
     7621 gacaacatca tcaatttgta cttcttttgc tattaaatcc caagggtcat tatttatgtc
     7681 ttttaatgcc aaggcaattt ttttagtctc tttatttaca tccaacacat aaacttctac
     7741 ttcttgtccc tctgaaacaa catcttcaac actttcaact cttgtccaag ctaaattatt
     7801 tatatgtgct aatccttcaa tcccaccaat atccacaaat gcaccataag gcattatctt
     7861 tgtcacctta ccatctcttt tttgtccaac ttcaagagaa ttaaataagt cttctttagc
     7921 tgctttgata cgagcttttt cttcttctct ttcttgctta attcttgctc tttcagcttc
     7981 tttttctgcc ttaatttgag ctcttctttc ttctctctct ttatttatac gctcttgtaa
     8041 tacatctcta tgagatgcta caagtctatt ttttctaaca tccaattctt ttatataaac
     8101 ttctaagtta agaccaacaa atttagatgt atctgtaata aattttgtat caagttgaga
     8161 aattggtata aaaaatgtat atgtattgta attagcaaat acacctttat ctatattctt
     8221 ttcaacattt acagttatta ttctatgttc atcataagcc ttttgtaact cggcaaaatc
     8281 atttcttttg tcaagttgta atttagataa agttattgta ccatcttttt gagatacttt
     8341 tgttattaca gcagttattt catcacctat gtggtatata tcttctatat attgattttt
     8401 ttctatgttc aattcactta taggtattat tccatcaaac ccatagtcta atcctaattg
     8461 tacttcatca tttctcactg cagttatttt tccagttgtc aattctccaa ctttcacttt
     8521 gctgaatact tgttcttgct ggtcaagtaa ttcttgcatt gttaaatcat tttccattat
     8581 aaacatcctt tcctcttatt gattatcata tctgtaaata ttataccatt aatattgcaa
     8641 atacttacat ttttcttact ataattttta aaataaattc aacttcatat atttatacat
     8701 tatagaaaat atcttattaa attaaaatac aaattctaat ttttatctaa taaaatattt
     8761 tcataaaaaa tttatattgt atatcttatt cttttattgc tttaggtttt gatagaaaac
     8821 atttataaaa tataaagaaa gaaattgctg ttatagtagc ggctgttata tctgcaactg
     8881 gctctgctaa aattactgca aaaagttttt cattaaagaa tgttggaagt ataaatatta
     8941 aaggtattaa aagtacaatc tttctaagta gagctaaaac caaagatatt gttgcttttc
     9001 caagtgctaa aaatgtctgt tgacaagcta tttgaatccc aaacataaaa gcaccagcaa
     9061 aatatatttt tatactccaa ctagtcatac taagtaactg cggattctta ttaaatatac
     9121 taacaaaaaa tgcaggaaat atcattaata aaagccataa aatagtagaa tatgtaaaac
     9181 atgacaataa acacaactta aatgtctttt ttactctatc aagttgacct gaaccataat
     9241 tataacttat tattggttgt gctccttgtg taacccccat attaggaagc ataagaattt
     9301 gcattatact gctcattatg gccattgaac ctactgctaa atctcctcca tacatttgta
     9361 atttactatt taaggctata agtactaaac tttctgtaga ttgcattata aatggagaga
     9421 ttcccaagcc aagcactggt aagatgatac ttgcttttgg tatcatatac ttctttttta
     9481 ttttaagtat acttttcttt ccaaagagaa acataagtac ccaaatagca gaaacgcctt
     9541 gtgcaattat agtagctaat gcagctcctt ttacacccat attaaagcca aatataaaaa
     9601 tcgggtcaag tattatgttt attaaagcac ctatagcaac tgtcatcatt ccagttgtag
     9661 caaacccttg ggtatttata aaagtattca tacccattga aatctgaaca aacactgtac
     9721 ctattaaata aatatttaaa tagtccattg aatatccaat tgtagattca cttgctccaa
     9781 acatccaaag taaaggctct tttccaatct taaaacctat agtcaaaagt attgctaaaa
     9841 tcaccaatac agaaaaactg ttactcatta ttttctctgc ctcatcattg ttttgctcac
     9901 ccatttttat agcagctaat ggacctcctc ccataccgat taaataacta catgcagtta
     9961 ttataattat tattggaaat gccactccaa caccagccat agcaacttct ccattttcca
    10021 ttctaccaat aaaaatccta tcgactatgt tgtataaaac atttacaatt tgtgcgataa
    10081 tagcaggtgt agcaagttta aataataatt tacccacact ttcactacct aagttagtgt
    10141 ttgaattttc cattttaaaa cctcctcaat ccaataatta aaatgtatca ctaaaatttt
    10201 attctatata aaaatttcat tttattagca gacaaattta gagtactttc cctacaagta
    10261 atactatact atatttcttt tgccatagta aacattttag aactaactga agttaaatac
    10321 tttatctcat tttttattta ttattaaaaa tttttcacat atactcataa aaaaactatc
    10381 caagtatatt acttagatag ttttatatta tatgtaaaga aagagctgaa actataaccc
    10441 ctgataacaa attgccaaga aatactccca taaaagcatc ttttattctc atttttaata
    10501 tggaagctat agcagatgca gtccatgttc ctgtagttgg cagtggtata cctacaaata
    10561 gtattatacc aattatcgaa acacttttta atttcttcat tcttgagttt atttttctat
    10621 caattacatc agcaatccct ttaaatagtt tcttccctct taaaaattgt aatacatgtc
    10681 tgaatgtcaa tatcaaggga atcgatacca atgtagaacc tattacacta aatacataaa
    10741 cccatatagg atttaagccc attgcaatac ctataggaat ggctcccctc aattctgtta
    10801 caggaaccat agataaaatt aatatgtgta aatatcccaa actattccct cctattctaa
    10861 gtgacattaa gttatgaatt tatttgctct ataacctcac catccttttt actaactaaa
    10921 aatgcatttt tagtcaatga aagcattggt aaatcactct tagagtctga atatgcatat
    10981 gaattttcat aatcaatctc tatcccaagt ttacctaata cagcctttat tctttttact
    11041 ttttcaattc ccttacagtt ttctccaatg aattgatttt tatatcttga attttcataa
    11101 aaaaactccg tgcctataac ctcgtctgca agaccataat acttaaaata tttcatatat
    11161 gcatatggag atgcagttat cataattata acattatctt cttctttatt tttgtaaata
    11221 acctttttca tagattcgtt aatttttttc attatgcaat catcaaaaaa cttttttaaa
    11281 tcttcttcac taaaatctac tattggataa aagattgcct cttttgcctt cttcaagggt
    11341 atcattctaa ttaaatacat aaatagaata aaaataatgt atggcaactt tataatagtt
    11401 ttaaaggttt tattttttaa agagtaaata aaaaaatcta tcatgctatc agtatttata
    11461 acagtataat ctacatcaag taataataca ctttttttca taagtatttc cccatcctat
    11521 cactaaaaat atcactaaaa aatcaatatt attaaacatg taattatcca taagattacg
    11581 tttattttta aagcattatc ttttaggaca atatcagtag gattattttc atttgtagtg
    11641 ttaattatat aattatatct aagtacacca taaactacaa atattgttgt aaatatcata
    11701 tgtggctttt ctggattact tgtagaatac aatgcataac atacaattgt agaacttaat
    11761 ataactatca tcatttgatt tagattttct atgtcataat cttccaatat ttttctatgt
    11821 tcaactgcat catctcttag agtttctatt tcttttttac gttttccaag agctaagtac
    11881 agagataaga aaaatgtaca taaaattatc caatttgata aactaacact tatggctaca
    11941 catcctgcat aaaccctaag tataaaacca agagatattg aaaacacatc taatagtatt
    12001 atattcttta gtttaagaga atataatata ttgtttatca aatagagact gagaattata
    12061 gaaagattaa aactaacaac tgttgataaa cagactgaaa ttaacgctaa tactacaaat
    12121 agtatattag cacttttttt gcttattctt ccacttgcca ctggtctatt acatttttta
    12181 ggatgttttt tatctttatc catgtctata gcatcattca gtgcatatac tgatgatgac
    12241 attaggcaaa aacacacaaa cgcaataaac gtatttaaaa ataaatttat atttagtatg
    12301 ttgtttgaaa aaattatagc tgaaaataca aagaaattct ttatatattg ctttattctc
    12361 attaacttta tataatctga aattttagta agaagcctta tacttgtatt ggtctgtttc
    12421 ataaacttac taccctttca atattatttt ttaaacagga tatgatttta tccctatgtt
    12481 taaaatttct ataaatccat tgacattact gattactata gtaaccaata acagcgatag
    12541 aaatatatta tacatattct tttcattttt tctaagggct ttcgtaaagt aagccaataa
    12601 aattataact gcaaaactaa aatttatagc cataatactt gaattgacta gattatatcc
    12661 tactatacca tgtaaagtta gattgaatat aattattccc atacatagct taggaactct
    12721 tttttctgaa ttttcaataa ctgataaaac acaaaaacat aaagctaata atgtaagaat
    12781 tactggtatt gtagcaactt ctccattttg caaaaataac ccttttttaa tacttgctcc
    12841 aggaaaataa aaaaccgaat acaataatct ctctataatc attgcaatct tttccaaaat
    12901 tggttctcca cttataaatt tcgtatcata ctgtattaca tttccaaggt tcataaaact
    12961 ctcataagac ttcgttgaaa ctacacaaat atatgccatt aggataaatg taattaaatg
    13021 ttttaacatt tttcctattt ttaatttaaa ctcaaatatc atattaaaaa aatacaaata
    13081 tatattacta atagttattc caaatagagg aatagctgct aattctacca aataagaatt
    13141 ttttcctttc atcttatttg caataactat gaaaaatatt aatgaaaatt ggctatatat
    13201 aaatctctct acatttaaag tcataaataa ctgtggaaaa gaaaatatca ttatcatagt
    13261 aagtaaaatt aaagttaatc tctgcatttt tatcttttta atatgattaa agactaaagt
    13321 tattgacata gcactagtta ttatttgtag acaaattagt ccaaacccat aggcactatt
    13381 aaaatctgtt gtcccaggat ttgctattga agtacaaaat aggtatattg gatatgcaaa
    13441 aattgatata attgctgaaa acaatatatg cttactattg tcttgactta tagcaaatac
    13501 atttaaattg aataaaactc ctgtatctgt atctaacaat acatcataag tattgtttaa
    13561 atcataaaat atagtagtct tagaaaaaaa tatcttagtt attattaagt aagttattac
    13621 aactaatata aacaaaaaca tctcgttttt atattttaat attttactca tcaatatatc
    13681 accatccatt cattatatag gatttgctga cacctacaat ttattcctta tatttaatag
    13741 ccatcttcat aatgttctta cttgttttat tgtaattgaa taactcttta tttacaacca
    13801 caatatttac tttattgatg ttataatcag tcaagtattg tttatataac atagattagt
    13861 gttgtataaa atgagaattt gtaagcaata ttaatgtaaa atatttgaaa aactttatat
    13921 aaaaatatac aatgttactt atggaattaa actataacaa attaaagcat aatttaagga
    13981 gacaaaatta taatctgtct ccttaaatat taaaactata ttttaaaaca ttactaaata
    14041 ctatacaaat ttcaaatata gtataaaatt aattagactt taacactatt agctccatct
    14101 tcttcccatt ttttaatttc ttttaatcta tctattatag gtaaatgctg agtacattca
    14161 acttcacatt ttccacaaga tatacattct ttagcttttg ccttactatt cattgtgtaa
    14221 ttccaatgtc ctaagccata aactaacttt gtcatttctt catcactctt tttaaacatc
    14281 tgcttttcat tataaaaaag catataggct ggggtattaa taccttttgg acatacttta
    14341 caataaccac aacctgtaca tatttcgttc atattcttat ttaatctagt ctcaatatca
    14401 tgtatatcct catctgaata aattctattt tcgtctgcaa ttttacatgc atcatctata
    14461 tcttgttttt tattaaaccc tatcaaagat atacttattt gtgaaattcc tatgttgaat
    14521 cttaaagctg attctatagt actttcgcca tcttttgata gaaaagaaag ttctttatta
    14581 tactttggaa tagttccacc acttaatgga ttcattgcca caacaccata tccattatca
    14641 acagcatatt tggcaccttt tagcctatat ggaaagttta atatatttat tcccatagtt
    14701 acaccttcaa acttattttc acttagaata tctataacct catctcctgg ttgatgagat
    14761 gaaaatacta tatggtctat caatccttct tctttacatt tcaataatcc ttcatattga
    14821 cctccaggtc tcattgctag ttcgtaatgt tccatctttc taatattcca aacatgataa
    14881 aaatttattt tagatacgcc taatcgttct attgattttt ttacagcctc tatagctttt
    14941 tctgatgtat cacatgcttt tggcttttct ttagttgata cataaaaatc ggtcttacct
    15001 tcctttatca tttgtctaaa ggctagtcca aatatatctt cacttctgtc atcacaatac
    15061 cctggtgctg tatcaaagta attaatacct ttttcatacg catatttgac taaatcagca
    15121 ttttcttgat tacttttttc taaatcaaac cttaatcctc catagccaac tactgaaacc
    15181 attttccctg tctttccata ttcaacgtat ctcattgtaa attcctcctt agatatatta
    15241 agacttaatt tctttataga aaatattata ccataaataa tttttataaa ttatttatgg
    15301 tataatattt tttttataaa ttatattttt ttataacttg tattttatag ctaagttaaa
    15361 aactataaaa aggttcaata tggactatta cttgagcatt ttcattaatt tcttcttgta
    15421 tttgtttctc aattttatgt gttaaatcat gagattgctc tacgcttata aatgggtcta
    15481 ccattacatg catgtcaata taaatagaac ttttgctacc tctacttctg atattgtgaa
    15541 catcccttac ctcattaaat tcaaacacaa tttcttttat ataatcctca tctacagcca
    15601 ctctatcgac caaaattcca atagatggcc taaatattcc ataagccgaa taaattatga
    15661 atgcagaaat aataatagga actatagatt ctattattac tggaaatcct aacttaacac
    15721 caactaaagt aaccaataca cctagagaca caaatatatc acttcttgta tgcaatgaat
    15781 ccgaaatcaa aacataacta tttagtttag tcccaattct atattcatac atgcatacaa
    15841 aaatatttat aaaaagtgta ataattaaag caattaaact ttctatagtt atagcaggta
    15901 caactggatt agctattctt aacactgcac taagaattat cttaattgct ataacaaaca
    15961 acataccact tataaataaa ctggttataa tttcaaactt tttatgtcca taaggatgat
    16021 ttttatcttt tggtttagat gcaaagaaaa tacctataag ccccactata ttagatgctc
    16081 catctgacaa agaatgaaat ccgtctgctg tcatactata gctttttatt tgattaccaa
    16141 taatgatttt taataatgct actgcaaaat tagcaaataa aattatccac aatacttgct
    16201 tcactttctt atagttatcc ataacaaatt ccccctccct tcgattcaat aaagaaagct
    16261 atctatcaat atcaatcaac cagacaataa aatcgtatat aagcacattt actatattta
    16321 tattcaaaat tggttgtact tgttcattct atttgaaact taattataaa taaaaatcgt
    16381 aggtatggtt ttggtataca taaaaataag agactgtctc aaaataaaga taatcttctt
    16441 ttttattgtt gaaatgataa aatatatttg atttcatata aaaaagagtg cctcatgaac
    16501 taaaaagttc attttgagac actctcttaa attttttgat ataaactatt aattttttat
    16561 actaagcctt ctttaactaa gaatgaatga gcaacttctt ctggtgattt accaagttca
    16621 tccacttgat agttaagttc tctcatagtt tcttcattta tttttcctga aagcatattc
    16681 ataacttttt ctaactcagg atatttcttc aaagtatctt ctcttactaa tggtgctgca
    16741 taatatggta caaagaaatt tttatcgtcc tctaatatct taagattaaa ctttttaata
    16801 agaccatcag ttaaaaatgc atctataacc tgagattcat tattatctaa agctgtatat
    16861 cttaaacttc cattcattgc ttttacatct ttgaatttta aaccataatc tctagaaagc
    16921 cctacaagac catcttctct attttcaaac tccaaagttg gtgagaatgt aaattcttta
    16981 ccatatttag tcaaatcaga aatagtattt attccatact tttcagcagt ttcaggcatc
    17041 atagctaatg tgtaagtatt attaaatcca agagggtcaa gtaaagttaa ctgattttct
    17101 ttttccatag tatcttttac aacattatat gcttcatcag catctttaat cggagcatgt
    17161 ttcattacat ttaccaaaag tgtacctgta tagtcaacat acatgtctaa ctcaccagat
    17221 ttaattgcat taaacgcaac agatgaacca cctagattta aattcttctt aactttaaga
    17281 ttcgtatttt tttctactaa ttcagcataa acattaccaa gtataagttg ttctgtataa
    17341 tctttagaac caatagttat tgtatctttt ttacttgaaa aactagaact aaaaacaacg
    17401 aacaacattg aaacaacaac cactacagat attactttaa gtgctatata attcttctta
    17461 ggtgcttttg gatttaatcc ttttggagta actgcaactt caattttact aaataaataa
    17521 tcaactaaca gtgcaattat acatgatggg attgcccctg ctaaaatcat attattattt
    17581 acagtttgaa cccctgaaaa tactaaatat cctaaaccac cagcacctat aaatgctgca
    17641 agagtcatca aaccaacagc tgtaactgca gatattctaa ctccacccat tattatagga
    17701 agtgctaaag ggaattgtat tttaaataat gtctgatttc ttgtaagacc aataccttta
    17761 gctgattcta atactactgg gtcaatactt gttataccta tatacgtatt ttttacaatt
    17821 ggtagtaatg aataaaccac taccataaat atggctggtt tacttcctat accaagtata
    17881 ggtacaagaa gacccaaaag tgccatagac ggaactgctt ggactaagtt tacaaatcca
    17941 agtacaaact ttctaagaga tgagattctt gaaataatta ttccaagagg aacaccaact
    18001 attatagcta ttaaaataga tgtaacagtt agactcatat gttgtattaa aagctctata
    18061 atcttatctt tttgtaatag tataaagtta aaaaaattag ccaagaacac tcacctccat
    18121 ttctaagaat tgttcactta aaacagaaag taaactactt ctagtaataa gccctaccag
    18181 tttattttca tcagaaataa ccggaatata tccaaccgaa ttacgattca ttacatttag
    18241 tatttctact aaattatccc cttcatttac atgcaaaggc tcacttgaca taatatcagc
    18301 aagtaatatt gacttatcat caatatcttt catatctttt acagtaacaa taccttttaa
    18361 tgtttttgct ctatctataa ttagtaagct atcaacctta ctcgttctca taatttctat
    18421 tccttgagtt acagttctag tagaattaac agcaatagga ttttttatca taatatctct
    18481 tgcttttata tattctggat tatcccaaac tctatcttct ccgataaaat cttttacaaa
    18541 gtcatttgca ggctttctaa gtatattttc gggagtatca tattgtgcta ttcttccatc
    18601 cttcataata caaattttat cagctatctt taaagcctca tccatatcat gcgttacaaa
    18661 tattatagtt tttttcaatt catcttgaag agaaaataat tgttcttgta aagatgttct
    18721 cgttattggg tctaaagcac taaaaggctc atccatcaat attatttcag catcagtagc
    18781 aatggctctt gcaacaccga ttctttgttg ctgtccacca ctaagttctg aaggatactt
    18841 attcaaaaat tcatcactat caagtcctac catctcaagt agttgcaacg ttcttttttc
    18901 aatttcttca acggattttt ccttttttag gcgtggaatt agctctatat tttcttttat
    18961 tgtaagatga gggaataggc ctgtgttctg tataacatat cctatttttc ttctaagctc
    19021 tatttcattt tcctgagaaa ttggtttgcc attaatatat atctcaccag atgtaggttt
    19081 tattaatttg tttataagct tcaaagtagt agtctttcca caaccacttg aaccaatcaa
    19141 aactaccaat gtacctgttt ctacaactag agaaatatca tctaatataa cattatttcc
    19201 tatttttttt gttacatttc ttatctctat cataatttat catccccttt ttcgaacaag
    19261 caaccttctc caatattata agttgttttt ataattatat catactattt tatttatgta
    19321 aacaaagtac gatttttagg tcactattta tctgaatatt gctacaaata taaagtattt
    19381 atatatagta tataatatac aatgtcaaaa aacaaaaaat ataaaaatat tcataaaata
    19441 acactagcct caattatata gtattaaagc tagtgttatt tcatttaaac aaaactcaca
    19501 tttggatact atccatgtat tcaaataatg taatatcttt ataagtttct tttttatcac
    19561 ttaggtttga tattgtaact cccaatagcc taatttgttt tccattaacc tcaacatttc
    19621 tcaacaaatt atctacacca cttcttatat cattatattc gtcaatatag tgttctaaac
    19681 taaggcttct agttatcttt gtaaagtcct catatttaat ttttaatgtt aatgtttttc
    19741 caaacttctc agagtttttt aatctatgac atacttcttc acaaagttca tcaagaatgt
    19801 tgcgtgtttc ttcttcatca atatccaaat tatgacttaa tgtagtctca gccccaacag
    19861 attttctaac cctatttggt tccactggtc tatcgtctat tcctctagca aatttatata
    19921 attcataacc tctttttttg aatatatttt caagttcaaa taaatttaaa caccttaaat
    19981 catatccagt ttttatacct aaatttttaa gagtatttga agtaactttt ccaacaccaa
    20041 aaaatttatt cacaggtaat ttatctaaaa agtcctgggc attttcttcg gttattacag
    20101 ttaacccatt tggctttctt aagtctgatg caatcttagc aagaaactta ttgtatgaca
    20161 caccagctga agaagtaaga ccaacttctc taaatatatc tttttttatt tgctttgcta
    20221 tctcaattga tgaatcaata ttttttttat tttttgttac atctaagtaa gcttcatcta
    20281 aagataatgg ttcaactaaa tctgtgtatc tataaaatat atctcttatt tgctctgaca
    20341 cactcttata aacatcaaat ctaggtctta caaaaactgc atatggacac ctattgtaag
    20401 ctacagctga tggcatagct gaatggatac caaattttct agcttcataa gaacaggttg
    20461 caaccacacc tcggctttgt ggatttccac cgacaataac aggtcttcct tttaattttt
    20521 tattatctct ttgttctata gatgcataaa aagcgtccat atctatatga attatttttc
    20581 tataatgttt cattattatc accttttaaa gtataaatag attaatttta tttatattac
    20641 tttttaatta taccaaactt atgtttcttt ttggtatttt ttcattattt tctttatata
    20701 ttcatatttt ccataaataa aaccatctat aatccattaa cttcaatata atttataaaa
    20761 aatccttaaa aacacttgca aaaactatat aaaagcttta caataggtat tccaaggaac
    20821 taaatttaaa attccttatg gataacagta tatatattta atttatggga tgttttaaaa
    20881 acaaaagact gtcttataca agacagccta ctgattttat tataattgta atggactgct
    20941 caaactatta ctttaaaaca gcccatattt ttttatataa ttttaactat agtaaagatt
    21001 ttgtgaagga tattctgggt cacaagttac atctatacca agttttctaa atgtttgttc
    21061 atcatctcta tttaatatag tagtagaatg agcttgacac ccttttaata atgctaactt
    21121 ttccatagca aattgtgcag ttggattagt tacagcacaa atactaagtg cagttaacac
    21181 ctcttggcaa ctaagtgaaa catttctact agctaaagtc tttgttttta agtttatgat
    21241 aggttctaat attacaggtg atattaagtg catatcatca gatatattgg ctaaatgttt
    21301 aatggcattt aatactactg ctgcagttcc attcattaaa tcagaaccct taccagttaa
    21361 tattgtacca tcttctaatt ctaatgcaac tacaggacaa gtatcattct tgtcggcact
    21421 ttctttcaat ttattactgt agtttctagc aggtaataca acatttctat cttctggctt
    21481 taagttaagc tcttccataa tcattttcat tctatcataa gcacctttat caacttgtcc
    21541 ctttttatac tcacaggcag ttttaaagta tcttcttatt atttcttcta tagaagcttt
    21601 tcttattact tcatcatcta ttattccaaa gccaactcta ttaacaccca tatcagttgg
    21661 agattggaaa attgattctt ttccagttat cttttctatt atttttttaa gcactggaaa
    21721 taattccaaa tctctattgt agtttactga catttcacca tacgtttcaa ggtggaatga
    21781 atctatcata ttcacatctt ttaaatcaac tgtagctgct tcataagcta tattcaaagg
    21841 atgttttaca ggtacattcc aaactgggaa tgtctcaaac ttagaatatc caactacatt
    21901 acctctttta ttttcatgat acaactgact tagacaagta ccaagttttc cactattagg
    21961 tcctggtgct gtgactacta ctattggctt tgtagtttct atatattcat ttgctccata
    22021 tccctcatca ctaactatag tctcaacatc tgacggatat ccctttgttg gataatgctt
    22081 ataaacttta attcctctac gttctaattt atttataaat acagttgtag caggttgtcc
    22141 ttcaaatcta gtaattacta cactatttac atctagttca taacctctta aatcatctat
    22201 taatcttaaa acttccatat cataagtgat accaaagtct cctcttattt tgtttctttc
    22261 tatatctcct gcatagacac aaattacaac ttcaactttt tctttcatat gttgtaatac
    22321 ttttatcttt gcattttcat caaatcctgg aagtactctt tttgcatgaa ggtctcccat
    22381 caattttcct ccaaactcta aataaagttt gtcaaaatta tttactcttt ctaagatata
    22441 tttagactgc tcttctaaat attttttgtg gtcaaatcct actttcatct taacacctca
    22501 ctaaattaaa tttcttgttg ataacagtat atttatatat atttttttag taaaacatct
    22561 tataaaaaaa tgttaatact ttttcactca aagtaatatt atacaattaa gattaatttt
    22621 tttcaagata gttttatggt aaataagata tacctatata actaataaaa taagaatact
    22681 atatttttat atataagtat aatattctca ccttagcatt aatttatttc aatattttca
    22741 tatatctttt attctaaatt caatactttt ctaatatctt ctataccttg ataaacttct
    22801 tgtttagaaa aattcatagc ttctaattct tcatcagtct tttccataag catagtataa
    22861 aattctattg ctatatactg catattttca ctatttacac tcttagcttt atcaaataaa
    22921 atttcttctg cttcgtttac atttccctga aaaactaatc tttttaaagt tataagtata
    22981 acttcttctt ctgtattata cactaaatct ttttcttcat attcactgta tttttttccc
    23041 aataaaagtc ttgcaaccat aagttttatc tcattgaaaa tcttatccac tatagccatt
    23101 tttacatctc ctataaataa attttatttt ctttttaatt atatcagact ttatcgtaaa
    23161 aattattata tgtaaatgaa ataattcaaa aataaatttt aacaataaaa aaaagaaggt
    23221 agcgggggat ttccgactac cttcaaacac tagattgtga ttggtttggt attttattta
    23281 tacccaccat aagctttatt aaatcatcta aattgatttt tttagcatcc acatcctcca
    23341 tcaaaaacgt caccacattc acaaagtatt aataataata ttaagaaagg tatccatgca
    23401 cataagtcca tttcagccca acaatcttgg aatgctaaga ataagaagaa taatattatt
    23461 atccaccaag cacatccacc aaatccgcct tcaccaaaaa atctatccaa cataatatgt
    23521 tacctcctag tcattttgta tgtggagttt cagctcctaa aatatactat gtttaagggg
    23581 taatatttgc taatgatttt tttaaatttt tttagatatt tttaaaaaaa ttattgattc
    23641 acactcaatg tcttaccatc actattgata actacctctc cattagaagt caaaaatgtt
    23701 tttatattca atctttccaa tatttttaaa acaccttcct ctggttcttt tttctctgaa
    23761 catgtaataa cagcatattg aggtgaaata gactctaaaa atgtttcagt taatttgtca
    23821 tatcttccat gatgaggcat tttaacaaaa tcatatttta aagtatttcc atttataaac
    23881 tctgacaatc tttcttcctc tgcatctcct gcaaataaaa acttatgtgc tccatagctt
    23941 atattagtga ttattgaata atcatttgaa ctctcgtact tgctttttga agccggatgt
    24001 atgtttattt ctgcaccatt gattacaata ttcatattat cttttagtag cactggttct
    24061 atatcagctt ttttaagtga gtcaatatat tcatcatatt gcttactatc tttcttataa
    24121 tttgcttgta taatattctt tactttaaca ctagataaca cactatctac tccacctata
    24181 tggtccttat ctaaatgagt taaaatcaaa tagtcaagtg tatttattcc tttttctttt
    24241 aacctatcca ctatttgttt tccattttta tcttctcctg tatcaatcat tatgactttg
    24301 ttacctatct caaccaatat gcaatcagac tttccagtgt ttagtatagt agtcttaaat
    24361 gcagtatttt ttgtgctact ctttgtgctc ctctggatac cattttgcct cacactattt
    24421 ttgtcacttt ttaaataaga ttcacttgtt gattttatct tactattagt agttatattt
    24481 gttacaagca ataatattac tattatagat aatgtagtaa ctaaaaacag gatatctttt
    24541 aatactattt tcctcatcca aaagctccta taaattttat ttattacata tttatagtat
    24601 agcaacctta actctgtaaa atacacatat aaaaagggca aattttaggc atatttgtgg
    24661 cataataaaa aaatgactgt accaaaacaa ttatctttct aaatttacat cttcaaattc
    24721 tttaatctta tcattagata aaacactctt tttagtagat tgtccatttt ttctatcaaa
    24781 agtatttttg gcttttacac ctgataaaaa cttaccggct ccattaatac aaccttcttc
    24841 acacatcatt ccttctataa aattaccttc taatcttccc aatttagcca ttgtcattgt
    24901 ctttttaatt tgtcttggac cagatacttt tataggttca aaatcaaaac taagcccctt
    24961 ttcagcaata tagttctgta ctgatttggc aagtccacca gatgctccaa atccccttcc
    25021 aaaaatcgaa ccatcattta tatcttcatt tgaacatttt gataaatcta tattaaatgc
    25081 ttcaaataat gccattagtt cttcaaaagt aagtacataa tctactgaat cttttaaact
    25141 tttctctgtt gcctcactct ttttagctgt acatggacct ataaatacaa ctttagcatc
    25201 agaatctatt ttttttacta atcttcctgt tgctaccata ggagacactg tagatgatat
    25261 tctatctttt tcatctggga acatcttctc tatatactta aaaaatcccg gacaacatga
    25321 atttgtcatg taattatctc ctgattttat tctttcaata agttcattgc tttcatgaac
    25381 tgtaactgca tctgctccac aagaagcttc tatcatatct acaaatccca gtgattttat
    25441 agcatttttt aactgaccat atgtaatatt actatctatt tgacctgtta tagctggagc
    25501 tactattgca tacatatttt ctttctttgc aagttttttt gcaacaggtg ctatcaaact
    25561 cttatcagat attgctccaa aaggacatgc tgacatacat gctccacaat ttatacaatt
    25621 atcttcatgt atcattgctt tcatagtgtt tctattaaaa tccaaagctc ctgttggaca
    25681 aacgctttta caaggtctta caacttctga tatagcatca tattgacatg aacttttgca
    25741 cattccacaa gccttacata aatcatgatt tatatatgct ctcccattta tataagaaat
    25801 tgccccaaat ttacatgctt ccttacatct atgagccaaa caccctctac aaagctctgt
    25861 aactgtaaat tcatttattg gacatctatc acatgctgct tcaactatat atataatttg
    25921 ctcatctgtg tccaaactaa ctgaatctgg atttatatct ccatatttac tatttggagt
    25981 taaacttaat gcaagttgcg ccctttcaag aactacttct ctctctttat aaacacaaca
    26041 tctatatctc gccttttctc ctttaattat ttcatatgga attttttcta tattttcttt
    26101 tgttaaattt tcttcaatag atagtcttgc tactgctgtt aaaacttcat gttttaacct
    26161 ttttagttgc gtttcaaatt caaacataag taatcctccc tataaatcac acctttttgc
    26221 tatatttcta ctattatgta tattgtattt tttatttatt tctataaatt attataacat
    26281 aggcatatac tttgttaagt tatatacaaa atattatata tgtaattttt attttaagtg
    26341 tatataattt ttattttagt tgttcaataa ttaaatatat aaataaaaat agagttgtcc
    26401 aaaagttaaa cctcaactta gaacaactct attaacaata tgtatttttg ttgctttgta
    26461 ttatctttat ttttctaaat ttatattact aaaatctttt atctttgtat ttgctaagac
    26521 acttttctta gttgattgag aattttttct atcaaaagtg gctttaacct ttgcacctga
    26581 taagaattta ccagaaccat ttatacaacc accttcacac atcattcctt ctataaaatt
    26641 accttctagc tttcctaaat tagccatagt catagttttc ttgatttctc ttgaaccaga
    26701 aatctttaca ggttcaaaat taacaccaat acctttttca gcaagataat tttctatagc
    26761 ttttgtaaga ccaccagcag ctccaaaacc tcttccaaaa attgatgcat catctatatc
    26821 ttcatttgaa cacttagcca agtctatatc aaatgcctca aataatgcca ttatttcttc
    26881 aaaagtaagt acataatcaa cagctccttt taattctttc ttagctgcct cactcttttt
    26941 ggctgtacat ggtcctatga atacaacttt agcatctggt tcatgacttt ttataaatct
    27001 accactagct atcataggag atactgtaga agatattttc cctacatgat ttgggtatgt
    27061 tttttctata taaccaacaa atcctggaca acaagaattt gtcataaaag cttctccctt
    27121 ttccattctt tcagcaaatt ctaagctttc atgaactgta actgcatctg ctccacaagc
    27181 tgcttctaat acatcaacaa atcctagtga ttttatagca ttttttattt gaccatatgt
    27241 aacatttgct tcaacttgac ctgttatagc tggagctacc accgcataca ttttctcttt
    27301 ttgaactaac tttctagcta caggagctat caaactctta tcagatattg ctccaaatgg
    27361 acatgctgac atacatgctc cacagtttaa acatttatcc tcatgtatca tagctttcat
    27421 attttctcta tcaaatccta aggcattagt tggacaaaca ctcttacatg gtctaacaac
    27481 ctctgagatg gcatcatatt gacaagcttt tttacacatt ccacaagcct tacataattc
    27541 atgatttata taggccattc ctccgacatg tgttatagct ccaaatttac aagcttcttt
    27601 acatctatgt gctagacatc ctctacaaat atctgttact gtaaattcat ttattggaca
    27661 actgtcacat gcagcttcca aaatatatat aatttgttca tcttcttcta tatccattgt
    27721 atctaaatct atcttatctt gacctggttg taaccccata cttacattta ctctctcaga
    27781 tattatagct ctttctttat aaacacaaca tctgtatgta ggtttctcac cttttattat
    27841 ttcatactga atattatcca ttgcttctct ggtaaggtta tcttcttttc caagtctagc
    27901 tattgcagtt aaaacatcat gtcttaattt gtgtatttgt gtttcaaatt gaaacatttt
    27961 aataccaccc ccgtaaatga ttttatacga taccattata acacaatatg tccttttatt
    28021 aatatataca taaaatatta tatgtttaaa t
//
LOCUS       DAMJVK010000048        27944 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.048, whole genome shotgun sequence.
ACCESSION   DAMJVK010000048 DAMJVK010000000
VERSION     DAMJVK010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27944
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.048"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            <1..410
                     /locus_tag="PC414_002132"
     CDS             <1..410
                     /locus_tag="PC414_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4004 family protein"
                     /protein_id="HDF2464527.1"
                     /translation="IANMFMKKDINKEFDIDFILSKEVICNYTKEIFQNLYKKEQSIG
                     KKELLILSIIEKFLVKSIITFEELKLIVNIIEENFTSIYNESGKIYLFRKFGVPFVVG
                     CLDYKKVKFEKDVVKIIEVDLTKEINDISIKLS"
     gene            complement(710..1297)
                     /locus_tag="PC414_002133"
     CDS             complement(710..1297)
                     /locus_tag="PC414_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HDF2464528.1"
                     /translation="MNLKDSKTKENLLRAFAGESQARNRYNISASVAKKEGLYIIESL
                     FNYTADQEKSHAKQFFSKLKEFAGENISISGAYPVDLYDKTAQHLKAAQHNELQEWDD
                     VYKNFGKTAREEGFESIAKLFENIAEIEKVHGDRFGRYATDLEEGTLFRRNEDIQWIC
                     THCGHIHIGKEAPKACPVCLHPQGYFLDFKNSLFE"
     gene            complement(1383..1820)
                     /locus_tag="PC414_002134"
     CDS             complement(1383..1820)
                     /locus_tag="PC414_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2464529.1"
                     /translation="MEDKYIVHFISKTKASMIKFIENKLSKNGLGELIPTHGNILTAL
                     YENNGILTMKEIAAKIGKDKSTVTVLVNKLINLGYLERQKCTNDKRITYIKLTKKALL
                     IEDTFNSISTQVKETAYHNITENEKQEFLRILKKINDNFKNAD"
     gene            1924..2922
                     /locus_tag="PC414_002135"
     CDS             1924..2922
                     /locus_tag="PC414_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HDF2464530.1"
                     /translation="MNKKFKSLYITEEEGNFRRNIVEREVDTLPEGEVLIKVKYSSLN
                     YKDALSANGNKGVTRKYPHTPGIDASGIVESSSCDDFKIGEEVLVTGYDLGMNTSGGF
                     SEYIRVPSSWVVKLPKNLSLKEAMDYGTAGFTAALSVYKFIDSVDKNMGDVLVTGGTG
                     GVAVIATKILIKLGYSVVVSTGKLEEQKEVLLNLGVKDVIHRSEVDDNSGRPLLRPRW
                     AGVIDTVGGNTLATAIKTTNYCGAVTTCGNAGGVDFTSSVYPFILKGITLYGIDSVDC
                     PMNLRLKIWDLLSNEWKLDDLDDMVNVVSLEELNDSIDSIMAGKHVGRTVVDLEKI"
     gene            complement(3042..3968)
                     /locus_tag="PC414_002136"
     CDS             complement(3042..3968)
                     /locus_tag="PC414_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HDF2464531.1"
                     /translation="MSKENKNVRIAVVQASPVIMDLEKTVEKALGLIKEAGRKGANIV
                     VFPEAFIPAYPRGLSFGFVVGSRTMEGREDWKRYYDNSVPVPSATTDLLGKAAQEAGV
                     YLSMGITERDGNDINCTLYCTNLFFSPEGKLIGKHRKLKPTGTERCIWGEGDGSTLTV
                     VDTPYGKMGSLICWENYMPLARTALYAKGVKLYIAPTADSREEWQATMKHIALEGRCF
                     VIGCNQYVEKNMYPTDLNYYKELDAEPEIMCPGGSCIVDPFGKYVAGPIFNKEEMLIA
                     DLDLEKIVLSRLDFDSEGHYSRPDVFELIVHE"
     gene            4353..5957
                     /locus_tag="PC414_002137"
     CDS             4353..5957
                     /locus_tag="PC414_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2464532.1"
                     /translation="MKEKIYYNGNIITMEDSICGDAILIKDKIIKKIGTKEEVFALKN
                     KDTEIIDLQGKTLMPSFIDSHSHLIAFATTLKLVPLEDATSFKDIVKKIQDFKESNNI
                     KKGDWIIGFSYDNNFLEENKHPDKSVLDSASSENPILISHASGHMGVANTLGLKQLGV
                     TNETRDPEGGHIGRVEGSEEPNGYLEENAFFNVASKIKQPSSNEIFNSIEKAQNIYLS
                     YGITTAQEGLMEENQFNILKAMANQNKLKMDVVGYVNLKKSKSVADNNREFIKKYINR
                     FKIGGYKIFLDGSPQGKTAWLSRPYENSDDGYCGYPIYKDEEVEKFIDISLKEKMQLL
                     THCNGDAAADQLIDAFEKVLNLKEQSSENHIRPVMIHAQTVREDQIDDMKVINMIPSY
                     FVAHTYYWGDIHIKNLGEDRAFKISPLKTTIEKGLIYTLHQDTPVIAPNMLETVWCAV
                     NRITKKGIQIGENEKISPLDALKGVTINAAYQYFEEDKKGSIKEGKLANLIILDENPL
                     TIDPMKIKDIKVLQTIREGEVLYSLK"
     gene            6773..8044
                     /gene="serS"
                     /locus_tag="PC414_002138"
     CDS             6773..8044
                     /gene="serS"
                     /locus_tag="PC414_002138"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDF2464533.1"
                     /translation="MLDIKRIRENLDCIKKAMERRGEKDFNLDEVVKLDDERRKILQE
                     VEVMKNELNTASKNIPNLIKEGKDVENEKIKLKELSDKIKVIDQNLKEVEDKMEYLLM
                     RIPNVPHPEVPQGETDEDNVEVRTWGKTTTFDFESKAHWEIGTELGILDFETASKITG
                     SRFTLYKGLGARLERALLNFYLDTNTKVNGYTEVIPPFMANRNSFLGTGQLPKFEEDM
                     FKIEGMDYFMIPTSEVPLTNIHANEILKFEQLPINYTAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVEMVKIVAPEESYNELEKLTNNAETMLQLLNLPYRVVKICTGDLGFTASFK
                     YDVEVWMPSYNRYVEISSCSNCEDFQARRAGIRFKRDKDSKAEYVHTLNGSGLAIGRS
                     VAAILENYQQEDGSVVVPEVLRPYMGVSVIK"
     gene            8322..9827
                     /locus_tag="PC414_002139"
     CDS             8322..9827
                     /locus_tag="PC414_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDF2464534.1"
                     /translation="MKIQNTEWGYIEWKHTYDENNPKQAMNIYIAVTMPGKKHFNHVH
                     YGQEQMIYILEGEGLYIINGVWKPFYQGMIFYIESGSTHETINTGDREIKELIVSNNV
                     DDVGESEVIDINPNNYLKKTLINYSESTLNLYAAVESIRGQFIDPFKIPLIIYDDSWN
                     IVLKNPYFPLFCFEKCNPMKFPQNCDCMNQKSSNQFVCEYGITIYNIPILYKSNSIGV
                     IRGGYVLLSDLNLDTEHNNLYDIPEGAARSIKRLLKQISKNIINFCSFNDIRKDLQEK
                     EKTIARTYHYGEQLEMNLKVAQDMVTNLRINHHFLFNTLNSMASIALDDGSYDLYSAI
                     IDLSRMFRYTMRSDLRFVELESEILYIKNYLNLQKLRYGDALKVKYLIPEKLYNLSVP
                     FNFIQPIVENAFTHGFRDIDTEKRIEIIARLDSQYAIIEIHNNGTILDGNNIDKIKAG
                     IRSNNGHGLSLIYTKFTSAYGNNFDMDIKSSDNEGTYIMIKIPIENYKEYV"
     gene            9941..10639
                     /locus_tag="PC414_002140"
     CDS             9941..10639
                     /locus_tag="PC414_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDF2464535.1"
                     /translation="MRRALIIDDEEAVLKIITHFIEMKDMPIKIVGKATSGDEAVDKI
                     IGLEPDIVFIDIQMPIYNGLEVIEKTSHLSKKINFVVITAFNYFEYAQKALRLNVKDI
                     LLKPLDMKEFTKSVEKIIGYQYTNNDLLNEILEYINLNYSNDLKLNDCARLFLTNPSN
                     ISRIFKSNLNTTFISYLNHIRIEKSIELLENTTKSINEIAEIVGYNSLNNFYKNFKIE
                     KGMTPKVYKLNTKN"
     gene            10924..12012
                     /locus_tag="PC414_002141"
     CDS             10924..12012
                     /locus_tag="PC414_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464536.1"
                     /translation="MSDKVNMKNIICIAGAYLATAIGSGFATGQEILQFFASQGYMGL
                     IGALISMVLFSFLGAEVITRGRELKLKEPTRIYTFYCGKILGKFYEWFSPIFLFGILI
                     VMISGAGSTLTEYYGINPYLGRVLMAVATLITVSMGLEKLVSILGRIGPVIAVFTIAV
                     GLISLFKHFDGLSMVGEAMKNIEVTKASESPIVSGVIYNTLNIIVMIVFLTGMGASIE
                     SKKDAFWGGLLGGIVFMTAGIVMYLAMISDIGNLYMKEIPSLYLADNISPIVGVCFSV
                     ALILGIYTTAVPLLWSVTNRIVEDEHPKFKLVTTVIAILACIGGFLPFGKLVNILYPY
                     TGYMGLLILVCMVYKAITCRKKSTQSQE"
     gene            12251..13441
                     /locus_tag="PC414_002142"
     CDS             12251..13441
                     /locus_tag="PC414_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2464537.1"
                     /translation="MHELILISEKYKEDILDFFTDIHMHPELSFKEFRTTKAIKDLLV
                     SLDIEILDLGMETGVVGLLKGKYDGPTVALRGDIDALPIYEEVDIKYKSRYDGIMHAC
                     GHDIHTSCLVGCAYVLSHIRDSLHGNVKFIFQPAEEVNKGAKMLVERGVMENPKVDAI
                     FGLHNHPDIPCGKIGVKLGGLMAAVDTIKIEVNGFGGHGGIPNRTIDPIVASSAIIMG
                     IQTIVSRNISPLESAVISIGTINGGTANNVISEKVDMTGTCRSFSNEVRKKISENLEN
                     IVCEIARGYQATAKLDYLFDLPAVINSKEMYAIACKSVCDLYSEDAIVDPIPSTGGED
                     FSIFMEKAPGFFYWLGVGNKEQDCIYQWHNPKFKADKNSILVGTNVLCQSVINYMDKL
                     KNKI"
     gene            13532..15034
                     /locus_tag="PC414_002143"
     CDS             13532..15034
                     /locus_tag="PC414_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HDF2464538.1"
                     /translation="MRALNVVERAGNKLPDPVTIFLLLCIIVVILSAVISNLGVEEIH
                     PSTKEVVKVVNLLEKEQIQSYLGSIVTNFQSFAPLGLVLVTMLGAGVAEKSGFMEVLM
                     KKGISKVPQKLVTVAIVFAGMLSHTAADVGFIILPPLAALVFLGIGRHPLVGMFAAFA
                     GVAGGFAANVMLSTTDVLLAGFTIPAAQMMDPSYQGNPAMNLYFAFISAIVLTFAGAF
                     VTEKFIAPRFTKYNDSTNDKDIQELSELENKGIKYALLSLLVVVVVIVALCIGDNAFM
                     KDPETGSILSSNAPLMKGIVPIITIIFLTPGLVYGKVSKKIKSDKDLVSMMGSSMSDM
                     GGYIVLAFIASQFINLFNLSNLGTILSITGAKLLAESGIPSYGLIIGFILLSGFINLF
                     VGSASAKWAILAPIFVPMFMLLDFNPALTQIAYRIGDASTNPISPLFPYFPVILAFAR
                     RYDKDIGIGTVISNMIPYSVVFTLIEIIILLLFMGIGIPLGPGGGISYVL"
     gene            15106..16362
                     /locus_tag="PC414_002144"
     CDS             15106..16362
                     /locus_tag="PC414_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDF2464539.1"
                     /translation="MLKTTKISDPELYKIVADELVRQEHNIEMIASESTAPTEVLELS
                     GCVFTNKTEEGYPGARFQAGSEEADKLETLAIKRAKEVFGAEHVNVQPYSGSTANYCV
                     YSSILKPNDTVLSMRLDQGGHLTHGSAVNFLHDIYKYEFYGVDPNTGRIDYDALEAKA
                     KECRPKLIIAGASSYPRLIDYERISKVAKEVGAYFMVDMAHVAGLVAAKVIPSPVPYA
                     DFVSSSTTKTFCGPRSGIVLCKAEHAKKLDKGVFPGTLGSIHLNTVAAKAFSLLYLST
                     DKFKKIMEQVVVNAQTLASELISHGFSIVSGGTDNHIVMVDLRSKNLTGKQFEKALEY
                     VGITVNKNVIPDDPQSPFVTSGVRIGLTSISQRGLKEKEVIQIAGIMNKVAENIDNKE
                     VLDECKAEAQELISKFPLYPEGYFED"
     gene            16534..19308
                     /locus_tag="PC414_002145"
     CDS             16534..19308
                     /locus_tag="PC414_002145"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HDF2464540.1"
                     /translation="MRYYNKPTKEVLKYLKTNPEIGLDDNEVEERKLRYGLNEFTIKE
                     GRTFWDELGESLTEPMILILIGAAVISSFVGELHDALGILGAIFIGISIGIITEGKSK
                     KAAHALSKLTENIEVKVLRNGKIIKISKNDLVPGDIVYIETGDMIPADGRLIQSINLK
                     LREDMLTGESDDVAKNADAVLDMEVVYSKTEIIEQDAIPAKQVNMVFGGTLVAYGRGI
                     MVVTHTGDKTEMGKIAQNLSNEDQQTPLQIKLGKLGAKIAGISGIIATLLCMFMIIQM
                     QRKGMLILDTSSILSFLQSLEPAKNAYMVCIALIVATVPEGLPTMINITLAITMQKMA
                     KINALVTKKEACETIGSVSVICSDKTGTLTQNKMMVEVAYVDGKYISGGEYQSNSYFE
                     QNCIVNSTADIEKEDNSFKYIGSATECALLLYHNDKNYNEMRKQTYLISQIPFSSEEK
                     KMSTLIRQEDSDILLSKGAPEVLLKKCSYVQQGKNIVPITPKVEKSILDEIKKLQIKS
                     MRTLGFAYKKMSNSKTEVAMTSEGELNLIGNSRSYMKEDNLVFSGFVGIVDPLREGVK
                     DSIDKAFNAGVDVKMLTGDNINTATAIGNELGLLNDGKKAVEATYIDVLTDKELREEI
                     KGISIVARSKPDTKMRIVSALQKSGEVVAVTGDGINDAPALSQADVGIAMGISGTEVS
                     KNAADIILTDDSFSTIVEGIKWGRGIYENFQRFIQFQLTVNIVAFIIAIISQLTGKDM
                     PFTTIQLLWVNIIMDGPPALALGLEPVRDYVLKRKPINRHSGIIARSMFVNIIINAIL
                     IITIVFTQSAFNILGATSEEQGTVIFSLFAFSALFNALNCREFGLNSTIPNFFKNKLA
                     LQIIVVTGIIQIIFTQVFQSFFNSVSLDFDMWIKIILFASTILLSNEFVKLILRTMKN
                     SRTMNFNSKN"
     gene            complement(19373..19558)
                     /locus_tag="PC414_002146"
     CDS             complement(19373..19558)
                     /locus_tag="PC414_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDF2464541.1"
                     /translation="MFFISDIYTKSPIKFDTPLQKEAYKILQKLDIDFECVDTDEAIT
                     MEDCVQINKKLNMKMVI"
     gene            19933..21111
                     /locus_tag="PC414_002147"
     CDS             19933..21111
                     /locus_tag="PC414_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDF2464542.1"
                     /translation="MFSKILVKTFIRDSENVQNTDVRNKYGYVAGVVGILSNLLLFVI
                     KVFIGMLTSSIAIMADAFNNLSDMASSAITMIGFKLASKPADKEHPFGHGRIEYLSAL
                     IVAFMVMLVGLQFVKSSIERIVNPIPVKFEVIPLILLIASIMIKIWLSRFNKFMGNKI
                     DSSALKAVSLDALGDVFTSSCVVISFIVARFTNFPIDGYVGIVVSLVILYAGFSLVKD
                     TINPLLGEAPDEEMVNSIIELLLSYKYIIGTHDLIIHNYGVGRCIASIHAEIPSNIDI
                     MEIHEIIDTAEREISEKLDIYLVIHMDPICLEDKEVMSAKKELEEILKKNSLVKSMHD
                     FRIVGKGSKKNLIFDIVVNPSEFSKNMSEDDLKEDITKLVKEINPEYNCVIVVDKDFI
                     "
     regulatory      21238..21323
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            21441..24359
                     /locus_tag="PC414_002148"
     CDS             21441..24359
                     /locus_tag="PC414_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021362860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HDF2464543.1"
                     /translation="MKKGNRKALLISLIMILSMVVSTIYPTVSYASELGENSQIQSGS
                     TNSSTGEEKESDNKKPEQTPEKDKATDNKKPEQTPEEEKPTDNKKPEQTPEEDKSTDN
                     KKPEQTPEGEKPIDNKKPEQIPEEDKSTDNKKSEQALEDEKPLDNKNTEKTPEEDNLL
                     ENENLLKVLEEELNEENEDYGFVVKINNNTIETESMKKISFNLTYTPTSKGIQAGDSI
                     TFKVPDVFNKVNLDYTSECFDKTESNGEYTLTFRELPNGQSVMQGKIGLEAYVKKVDE
                     DTNAKIHIETTGKIESGSGDIDVEIKPGDKTDVPDAKGTLKKLVEGKKSTTVFMPVKN
                     KDINYSIQVNEKQEELKDIILYDELPEGLTLINGSVSVVTSDGKEVSDFNIEQSKNSI
                     SVNLGNIDKSYTVKYKARISDKNAKHGNKYKNVARIESDGKKIQEDDATVSIFDRGDD
                     YLLTKGHSGATNITQVGQVINYQISINDDKSPISNVVITDNIPEGMRLTTSGEAGHDF
                     RVVEIPMNGSWTPWSKEKIANNISYKVEEKRNESGQVDKVITGFTINLSKEEVESKFF
                     IAYTLKVISIEDSYINRAVLDANNSEIDKNDEINFKKNSGLISAKKEVDKKVLNSSDN
                     QIVKYKINMSTYGVYDAGQVNLLDEVNSALEISNIKYSDNLELKKEAGDGKNTIRLVN
                     KYEFKQIKEGEPVQSWVTFDADFTNVKVGETIRNVAQINGSSPPGVETTKQGYAFEAK
                     KVDALDKNVLSGAKFNLEDAFGNIVVKDLVSDEDGIIQSSIKKPGTYYLVEIMAPSGY
                     EKLKDKIKVEIGNKDIGRIVDIGNIENIRQENPPVNPPAPPNTDEPIVNPPVPPSTDK
                     PRKPSSSSDTEDTIVINPPIPPSEDIINPPIPEVLNPPVPPSEEMIETPVKQIIPIPE
                     VVKPSVSEEKNNKVKDDTLVNPPVPPKTGDSTTIIGEILLVIGAIVGLIVLRRNKNTN
                     "
     regulatory      24545..24630
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            24730..25392
                     /gene="zmp1"
                     /locus_tag="PC414_002149"
     CDS             24730..25392
                     /gene="zmp1"
                     /locus_tag="PC414_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease Zmp1"
                     /protein_id="HDF2464544.1"
                     /translation="MRPSKKLLIAIISIFLISSVPVSAHADSTTIQQNKDTLSQIVVF
                     PTGNYDKNEANAMVNRLANIDGKYLNALKQNNLKIKLLSGKLTDEKEYAYLKGVVPKG
                     WEGTGKTWDDVPGLGGSTVALRIGFSNKGKGHDAINLELHETAHAIDHIVLNDISKSA
                     QFKQIFAKEGRSLGNVNYLGVYPEEFFAESFAYYYLNQDTNSKLKSACPQTYSFLQNL
                     AK"
     gene            complement(25514..26476)
                     /locus_tag="PC414_002150"
     CDS             complement(25514..26476)
                     /locus_tag="PC414_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDF2464545.1"
                     /translation="MKKGYVYIILTTLFFSSMEISLKTVTNDFNPLQITLSRFFVGGL
                     VLLPFAVKRLRALSLSITKSDLKYFAFLGFMCVVVSMSLYQLAVLNTKASVVAVLFSC
                     NPVFVMLLAYLILKEKIYRHNVISLILEVLGIIVIINPLHTKLTLSGIFLTLSSAIIF
                     SMYTVFGKRKTLKFGGIVVTCFSFIFGSLEMLILVLLTKINFIADILNKNKLHVFANI
                     PIFSGYTLHNMPIMIYVFVFVTGVGYALYFMAMEATSTSLTSLVFFFKPVISPILALL
                     ILKEIIPINMVIGILLIVIGSIISIIPTIITQMHNKHVEALYKE"
     gene            complement(26646..27836)
                     /locus_tag="PC414_002151"
     CDS             complement(26646..27836)
                     /locus_tag="PC414_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2464546.1"
                     /translation="MISNKMQTLVANSSVIRAMFEEGKKLSDIYGEENVFDFSIGNPS
                     VEPPETIKAVINDILNEESPNLVHGYMNNSGYEDVRDAIAEHINKKDGLNLTRENLIM
                     TCGAAGGLNIILKTLLNPGDEVIAFAPYFGEYKNYTENYDGKLIEVPTNIETFEPDLD
                     ALKNAITPKTRALIINTPNNPTGVIYSEELLKNLGELLDSKQKEFNTSIYLISDEPYR
                     EIIYDGAKVPCVLKYYRNSFIGYSYSKSLSLPGERIGYIVANGQMDDFDDVMSSLNVA
                     NRILGFVNAPSLFQRVIARSLDAEVDVNIYKKNFDLLYNSLIDMGYSCVKPNGTFYLF
                     PKAPIEDDKKFCNDAKQFNLLLVPGSSFGCPGHFRVSYCVSYDKVKSSLPAFEKLAKL
                     YNLK"
BASE COUNT        10902 a         3280 c         4659 g         9103 t
ORIGIN      
        1 atattgcaaa tatgtttatg aaaaaggata ttaataaaga atttgacatt gattttattc
       61 tcagtaaaga agttatatgt aattatacca aagaaatatt tcaaaattta tataaaaaag
      121 aacaaagcat aggtaaaaaa gaactgctta tattgagcat aatagagaag tttttagtta
      181 aatctataat aacttttgaa gagttaaaat tgatagtgaa tataatagaa gaaaatttta
      241 catcaatata caatgaaagt ggaaagattt atttgtttag aaagtttgga gttccattcg
      301 tagtaggatg tctggattat aaaaaagtaa agtttgaaaa agatgtagtt aagattattg
      361 aggttgactt gacaaaagaa ataaatgata taagtataaa gttatcatag tgtattatct
      421 tatataatgt attataataa tctacttaaa atacacttta tttaaatatt tagtttaaag
      481 atgagatttt atgtgagtgt gtaaaagtta aagatagatt taagtaggtt atagggatta
      541 gtaaacaata aaatgaacca tgatgaacta ctataaattg tatttaaact acattttcta
      601 cttatagtaa tatcaatggt tcattttatt gttattaaga taatacagta tttataaaaa
      661 tacttgatat aaatatagta aattgtttaa atagaattat tgccttaatt tattcaaata
      721 atgagttttt aaaatctaag aaatatcctt gtggatgaag acatacagga caagctttag
      781 gagcttcttt acctatatgt atatggccac aatgtgtaca tatccattgt atatcttcat
      841 ttcttctgaa taaagtacct tcttctaaat cagtagcata tctaccaaat ctatcaccat
      901 gaactttttc tatttcagct atattttcaa ataacttagc tatagattca aatccttctt
      961 cacgtgctgt ttttccaaag tttttatata catcatccca ctcttgaagc tcattatgtt
     1021 gtgcagcttt aaggtgttgt gctgtcttgt catataaatc tacagggtat gctcctgata
     1081 tacttatatt ttcaccagca aattctttta atttagaaaa gaattgtttt gcatgtgatt
     1141 tttcttggtc agcagtgtaa ttaaataaag actctattat atataatcct tctttttttg
     1201 ctacagatgc agaaatattg tatctatttc tagcttgact ctcacctgca aaagctctta
     1261 ataaattttc tttagtttta ctatctttta aattcatatt ctacctctct atcacaataa
     1321 atttttacaa tatttgattc tttttattta ttattccaat aggagatata attatgctca
     1381 atttagtcag catttttaaa attatcatta atttttttta aaattctaag aaattcttgt
     1441 ttttcatttt ctgtaatatt atggtatgcc gtctctttaa cttgtgttga gatgctatta
     1501 aaagtatctt ctataagcag tgcctttttt gtaagcttta tatacgtgat acgtttgtca
     1561 tttgtacatt tttgtctttc taaatatcct aggtttataa gtttattaac caacactgta
     1621 acagtggatt tatcttttcc tattttagct gcaatttctt tcattgtaag tattccatta
     1681 ttttcataaa gtgcagttaa aatatttcca tgagtaggta ttaactcacc taaaccattt
     1741 ttactgagtt tattttcaat gaatttaatc atactagcct ttgttttact tatgaaatgt
     1801 acaatatatt tatcttccat aaattattct ccaatcttta atctatttaa attagtttga
     1861 cataaaacta tttataaact ataattagtt taacataaaa ctaaatgtat tgggaggaaa
     1921 tttatgaata aaaaatttaa atcactatat attacagaag aagaaggaaa ttttagaaga
     1981 aatatagttg aaagagaagt ggatacattg ccagaaggcg aagttctaat aaaagtaaaa
     2041 tattcttctc taaattacaa ggatgcactt tctgcaaatg gtaataaggg tgttacgaga
     2101 aagtatcctc atacaccagg aattgatgca tctggtatag tagaatctag tagttgtgat
     2161 gattttaaaa ttggagaaga agttttagta acaggttatg acttgggaat gaatacttct
     2221 ggtggttttt cagaatacat aagagttcct tctagttggg tagttaaact tcctaaaaat
     2281 ctttcactaa aagaagcaat ggattatggt acagcagggt ttacagcagc tttatctgtg
     2341 tataaattta tagattctgt agacaagaat atgggagatg tattagtcac tggtggtact
     2401 ggaggagttg ctgtaattgc aactaaaata ttgattaaat taggttattc agttgtagtt
     2461 tcaacaggaa aattagaaga acaaaaagaa gtattactaa acttaggtgt taaagatgtt
     2521 atacataggt cagaagtgga tgataactca ggaagacctc tgcttagacc aagatgggct
     2581 ggagtaatag atacagtagg aggaaatact ctagcaacag caataaagac aactaattat
     2641 tgtggagcag taacaacttg tggtaatgca ggaggagttg attttacaag ttcagtatat
     2701 ccatttatct taaagggaat tactttatat ggtatagatt ctgtggattg cccaatgaac
     2761 ttaagattaa aaatatggga tttattatca aatgaatgga aattagatga tttagatgat
     2821 atggtaaatg tagtatcatt ggaagagtta aatgatagta tagattcaat aatggcagga
     2881 aaacatgttg gaagaacagt agttgattta gaaaaaatat aggtgaataa atttataatt
     2941 aggattatag gttttaagca aaaatgagat acttgaaatt tacttgtgag tatctcattt
     3001 ttttatgtaa aaagatttat gtagatgttt gtgtaaaaag attattcatg aacaattaat
     3061 tcaaatacat ctggtcttga atagtgacct tctgaatcaa aatctagtct actaagtact
     3121 attttttcta ggtctaaatc agctattagc atttcttctt tattgaaaat aggacctgct
     3181 acatattttc caaaagggtc aactatacaa cttcctcctg gacacataat ttctggttct
     3241 gcatctagct ctttatagta gttaaggtca gttggataca tatttttttc aacatattga
     3301 ttgcatccaa ttacaaaaca tcttccttct aaagcaatat gcttcatagt tgcttgccac
     3361 tcttctcttg aatctgcagt aggggctatg taaagtttta cacccttagc ataaagagct
     3421 gttctagcta atggcatata attttcccaa caaatcaacg aacccatttt tccatatgga
     3481 gtatcaacta ctgtaagagt acttccatct ccttctcccc aaatacatct ttctgttcct
     3541 gtaggtttaa gttttctgtg tttaccaatt agcttacctt ctggagagaa aaataagttt
     3601 gtgcaataaa gggtacaatt tatatcatta ccatctcttt ctgttatacc cattgataga
     3661 tatactcctg cttcttgtgc tgcttttcct aataaatctg ttgtagcact aggaactggt
     3721 acagaattat catagtatct tttccaatct tctctacctt ccattgttct acttccaaca
     3781 acaaatccaa atgaaagacc tcttggatat gcaggtatga atgcttcagg gaaaactacg
     3841 atatttgccc cttttctacc agcttcttta attaatccta atgctttttc aacagttttt
     3901 tctaaatcca ttatgactgg tgacgcctgg acaacagcaa ttcttacatt tttattttcc
     3961 ttgctcataa aaacctccta aaaaatattt aaattttttg cattgaatag attacatgag
     4021 atgttatttg agaactaaga tacttatatt gtgaataata atgtaactgt atatttaaaa
     4081 taatacatca tgatttaaaa aattacaatt gtacagatat aattgagttt agatatttaa
     4141 atttaacaac ttataatatt ttttcaatta gtataaagcc tattaatttg tcttatttac
     4201 aatgaatagt atttacttct tggcaaacaa taattaaaaa taaaactttt atatgattaa
     4261 actctaaaga aaaagtttat aaagaagata atataattaa gcagaaaata atgaaaaaaa
     4321 tttattaaag tagacaacgt aaggagaatt ttatgaaaga aaaaatttat tacaatggaa
     4381 atatcatcac aatggaagat tcaatatgtg gagatgctat tcttataaaa gacaaaataa
     4441 taaaaaagat aggaacaaaa gaagaagtat ttgctttgaa aaataaagat acagaaatta
     4501 tagatttaca aggtaagaca ttgatgccat cttttataga ttcacatagt cacttgatag
     4561 cttttgctac tactctaaag ctagttcctt tagaagatgc aaccagtttt aaagacatag
     4621 taaaaaaaat acaggatttc aaagagtcga ataatataaa aaaaggagac tggataattg
     4681 gattttcata tgataataat tttttagaag aaaataagca tccagataag agtgtactcg
     4741 atagtgcatc ttcagaaaat ccaatactaa tttcacatgc ttctggtcat atgggagttg
     4801 caaatacatt ggggttaaaa cagcttggtg ttaccaatga aacacgtgac ccagaaggtg
     4861 gacatatagg tagagtagaa ggcagtgaag aaccaaatgg atatttagaa gaaaatgcat
     4921 tttttaatgt agcatctaaa ataaaacaac catcatcaaa tgagattttt aattcaattg
     4981 aaaaagcaca gaacatatat ctaagctatg gtattacaac agctcaagaa ggtttaatgg
     5041 aagaaaatca atttaacatt ttaaaagcaa tggcaaatca aaataaatta aaaatggatg
     5101 ttgtaggata tgtaaatttg aaaaaaagta agtctgtagc agataataat agggagttta
     5161 taaaaaaata tataaataga tttaaaatag gaggttataa gattttctta gatggttctc
     5221 cacaaggtaa gacagcatgg ttgagtagac catatgaaaa ttcagatgat ggttattgtg
     5281 gatatcctat atacaaagat gaagaagtag aaaagtttat agatatttca ctaaaagaaa
     5341 agatgcaact cttaactcat tgtaatggag atgcagcagc agaccagttg atagatgcgt
     5401 ttgagaaagt tttaaattta aaagagcaaa gtagtgaaaa tcatataaga ccagttatga
     5461 tacatgctca aacagtaaga gaagaccaga ttgatgatat gaaagtaata aatatgatac
     5521 cttcatactt tgtagctcac acatattatt ggggtgatat tcatatcaaa aaccttggag
     5581 aagatagagc gtttaagatt agcccactta aaacaactat agaaaaggga ttaatttaca
     5641 ctcttcatca ggatacacca gtaatagcac ctaatatgct ggaaacagta tggtgtgctg
     5701 tcaatagaat tacaaaaaaa ggtatacaaa taggagaaaa tgaaaagata agcccattag
     5761 atgctttaaa gggtgttact ataaatgcag catatcaata ttttgaagaa gataaaaaag
     5821 gttcaattaa agaagggaaa ttagctaatt tgataatctt agatgaaaac ccacttacaa
     5881 tcgaccctat gaaaataaaa gatattaaag tattgcagac tataagagaa ggagaagtat
     5941 tgtattcttt gaagtagatg aaagttagat attttataat ctataaaaaa tagttttata
     6001 taataagtta ggtagaatta aataagatta cagttaaatc aacaattata tttttattaa
     6061 attttaatta ttgtattgaa gatttataag tataatttta ttgattctac ctttttaaat
     6121 aactgtcgat ttattaaagt tatctatgta ctatattaaa caattaagta gttattaatt
     6181 gaacttatta gtttattaaa aaactatgta tattactttg cagtaagact ttggaactaa
     6241 atctataata gcaaatttaa atttattaaa tagattacct tataacattt catataaaat
     6301 atattgtgaa tctgagaaaa ttactatttt tctttgagta ttttttgtaa gcagttctaa
     6361 aagattcaat ttcataccta ttacttgaat cattcattaa aataattcta atttaaaaaa
     6421 taattctatc caaataatag atatatagga ctatgttata atataaaaaa atttatataa
     6481 aagttaataa ctatacaaga ttgataaaga aaaagtcaaa atagataatt gattagcaaa
     6541 atagatataa ttataatgaa gttaaatcta gcaatattct cctaaaatta aattacattt
     6601 atttaattaa atataatttg acaatggaca aaaaagtgag taaaatataa ataaaataaa
     6661 aataacaggt tcggataaag aaaaaggttt cttaacctca agcataaagt atattatcta
     6721 agttatttga gattaagaga taaatccaat cttaggtact gaggaggaaa aaatgcttga
     6781 tataaaaaga ataagagaaa atttagattg tataaaaaaa gcaatggaaa gaaggggcga
     6841 aaaagacttt aaccttgatg aagtagttaa gttagatgat gaaagaagaa agattcttca
     6901 agaagttgaa gttatgaaaa atgaattaaa tacagcatct aaaaatatac caaatcttat
     6961 aaaagaaggt aaagatgtag aaaatgaaaa gattaaacta aaagagttat ctgataaaat
     7021 taaggtaata gaccaaaatc ttaaagaagt agaagataag atggaatacc tattaatgag
     7081 aataccaaat gtaccacatc cagaagtacc acaaggtgaa actgatgaag ataatgttga
     7141 agtaagaact tggggaaaga ctactacctt tgattttgaa tctaaggctc attgggaaat
     7201 tggaacagaa ttaggtatac ttgattttga aacagctagt aaaataactg gttcaagatt
     7261 tactctttac aaaggattag gtgctagatt agagagagca ttattaaatt tttacttaga
     7321 cacgaataca aaagtaaatg gatatacaga agttattcca ccatttatgg caaatagaaa
     7381 tagtttttta ggtacaggac agttacctaa gtttgaagaa gatatgttta aaatagaagg
     7441 tatggactat tttatgattc caacatcaga agttccttta actaatatac atgctaatga
     7501 aatacttaaa tttgaacaat taccgataaa ttatacagct tatactccat gttttaggtc
     7561 agaagctggt tctgctggaa gagatacaag aggtttagta agacaacatc agtttaacaa
     7621 agtagaaatg gttaaaatag ttgcaccaga agaatcttac aatgagttag aaaagcttac
     7681 taacaatgcg gaaactatgc tacaattatt gaatctgcct tatagggtag taaagatatg
     7741 tacaggtgat ttaggattta cggcttcttt taaatatgat gtagaagttt ggatgccaag
     7801 ttacaatagg tatgttgaga tttcatcttg ttcaaactgc gaagatttcc aagctagaag
     7861 agcaggaata agatttaaaa gagataaaga ttctaaagct gaatatgtac acacattaaa
     7921 tggctctggg ttagctattg gaagaagtgt tgcagctata ttagaaaatt atcaacaaga
     7981 agatggttct gtggttgttc ctgaagtttt aagaccatat atgggagttt ctgttattaa
     8041 gtaagagtta aattatgtat ttttaagcaa tacaataata tagaggtaat aaaaaataat
     8101 taatataaaa gtgatacaat aatatagaag taataaaaaa atagttaata taaaagtgat
     8161 acaataatat agaagtaata aaaaaaaata attaatataa aaatgtgtac aaactaatga
     8221 atattgatat agtaagctat catatgaaag agtatgtaca catttttttt actaattttt
     8281 cacaatatga acataaataa tttacaggaa aggaatcttt tatgaaaatt caaaatactg
     8341 agtggggata tattgaatgg aaacatactt atgatgaaaa taatccaaaa caagctatga
     8401 atatatacat tgcagtaact atgcctggta agaaacattt taatcatgtt cattatggtc
     8461 aagagcagat gatatatatt ttagagggag agggtttgta tattataaat ggagtttgga
     8521 agccatttta tcaaggtatg attttttata tagaatctgg tagcacacat gaaactataa
     8581 atacaggtga tagagaaata aaagaactta ttgtttcaaa taatgtagat gatgtaggag
     8641 agagtgaagt tatagatatt aatcctaata attatttaaa gaagaccttg ataaattata
     8701 gtgaaagtac attaaatttg tatgcagcag tagaatcaat tcgtggtcaa tttatagacc
     8761 cgtttaaaat tccattaatt atatatgatg attcatggaa tatagtttta aaaaatcctt
     8821 attttccact tttttgtttt gaaaaatgta atcctatgaa atttcctcaa aattgtgatt
     8881 gtatgaatca aaaaagtagc aatcaatttg tatgtgaata tgggataact atatataata
     8941 taccaatact gtacaaaagc aatagcatag gtgttataag aggagggtat gtactattat
     9001 ctgatttgaa tttagataca gagcataata atttatatga tattccagag ggagctgcta
     9061 gaagtataaa acgcctcttg aaacaaatat ctaaaaatat aataaatttt tgctctttta
     9121 atgatataag aaaggattta caagaaaaag agaagactat agcaagaaca tatcattatg
     9181 gagagcaact tgaaatgaat ttaaaagtag ctcaagatat ggttactaat ttaagaataa
     9241 atcatcactt tttatttaat acattaaatt caatggcaag catagcacta gatgatggta
     9301 gttatgattt gtatagtgca ataatagatt tatcaagaat gtttagatat acaatgaggt
     9361 ctgatttaag atttgtagaa ttagaatctg aaatattata cattaaaaat tatttgaatt
     9421 tacagaaatt acgttatgga gatgcactta aagtaaaata tcttattcca gagaagttat
     9481 ataacctttc tgtgcctttt aactttattc aacctattgt agagaatgca tttacacatg
     9541 gatttagaga tattgacaca gaaaagcgaa ttgaaataat tgcaagatta gattctcagt
     9601 atgctattat agagattcat aataatggaa ctatcttaga tggaaataat atagacaaaa
     9661 taaaggcagg tattcgtagt aataatggtc atggtttgtc tttaatatat acaaaattca
     9721 catctgctta tggaaataat tttgatatgg atataaagtc gagtgataat gaaggaactt
     9781 acataatgat taaaattcct attgaaaatt ataaggagta tgtatagaac ttgtgaaaaa
     9841 ttaataagtt ttataaatgt taagaaatat attagatgtt tttatgttgt tttatatatc
     9901 ttactttttt atattattaa gctaaaaggg aggtattctc atgagaagag cactaataat
     9961 agatgatgaa gaagctgttc taaaaataat aacacatttt atagaaatga aagatatgcc
    10021 aataaaaatt gttggtaaag caacatctgg tgatgaagca gtagataaaa tcattggttt
    10081 agagccagac atagttttta tagatattca aatgccaata tacaacgggt tagaagtaat
    10141 agaaaaaaca tctcatttat ctaaaaaaat aaactttgta gtaataactg catttaacta
    10201 ttttgaatat gctcaaaaag ctttgagatt aaatgtaaaa gatatactcc ttaaaccatt
    10261 ggatatgaaa gaatttacta agtctgtaga aaaaattatt ggatatcaat ataccaataa
    10321 tgatttatta aatgagatac tggaatatat aaatctaaac tatagcaatg acttaaagtt
    10381 aaatgattgt gctagattat ttttaaccaa tccatctaat atatctagga tttttaaaag
    10441 taatttaaat acaactttta tatcatatct aaatcatata agaatagaaa aatctattga
    10501 gttacttgaa aatactacaa aaagtataaa tgagattgca gaaatagttg gatataatag
    10561 cctaaataac ttttataaaa actttaagat agaaaaagga atgactccaa aagtttataa
    10621 actgaacact aaaaattgaa attgataaat aaaaagtcaa tatagataat tgtttgagtg
    10681 tgtctgaata agtatacttt aattatgata aaaattaaag cgctttaaac atatttaata
    10741 atattaaacc ttataaaagc tgtacctatt tatataaaaa aatatatata aataaaaaga
    10801 aaaagatata caaaataaaa aaagtgaatt aaatactgtt tcaaaaagac agaattttca
    10861 tttaatttac tgagagttta tatttcttaa aggaaggtaa ctttataatg gaggtaattt
    10921 aatatgtcag acaaagtgaa tatgaaaaat attatctgta ttgcaggcgc ttatctagca
    10981 acagcaattg gttctgggtt tgctactggt caagaaattc tacaattttt tgcctcacaa
    11041 gggtatatgg gtttaattgg agcattaata tcaatggtat tgttttcatt tcttggtgct
    11101 gaagttataa ctagaggtag agaattaaaa cttaaagaac caacaagaat ttacactttt
    11161 tattgtggga agattttagg aaaattttac gaatggttta gtccaatatt tttatttggg
    11221 attcttatag taatgatttc aggtgcaggc tcaactttaa ctgaatacta tggtataaat
    11281 ccttacttag gaagagtatt gatggctgtt gctactttaa ttacagtgtc tatgggttta
    11341 gaaaaattag taagcatact tggacgtatt ggaccagtga tagcagtatt tacaattgct
    11401 gttggattaa taagcttgtt taagcatttt gatggtttat ctatggttgg agaagctatg
    11461 aaaaatattg aagttacaaa agcatcagaa agtccaatcg tttctggtgt aatatataat
    11521 acattaaata ttattgtaat gattgttttt ttaacaggaa tgggagcttc tattgaaagt
    11581 aaaaaggatg ctttttgggg aggtttatta ggtggaatag tgtttatgac tgctggtata
    11641 gttatgtatt tggctatgat ttcagatata ggaaatttgt atatgaaaga aataccatca
    11701 ctttatttag cagataatat atctcctatt gtaggtgtat gtttttcagt tgcattaata
    11761 ttaggtatct acacaacagc tgttccactt ttatggtctg taaccaatag aattgttgaa
    11821 gatgaacatc ctaaatttaa attggtaaca acagtaatag ctattttggc ttgtatagga
    11881 ggttttttac cgtttggtaa actagtaaac attttatatc catatacagg ttatatgggg
    11941 cttttaattt tagtctgtat ggtgtataaa gcaattacat gtagaaaaaa atcaacacaa
    12001 agtcaggaat agagccactt attaatagtt tttatttaaa gaaatttttt atataatccg
    12061 aatttaaaaa gtcaaaatag ttaaataaat tgtaaaatat gataatatat ttaattaaaa
    12121 taatatagta aaatataaat ataaactaaa attaaattta taaaaaatat ctttataaag
    12181 attattaact tatataattt gtagttaaat tataaaaaat aaaaaaataa aattttggag
    12241 aggtgttagt atgcatgaac ttattttaat ctcagaaaag tataaagaag atattttaga
    12301 tttttttaca gatattcata tgcaccccga attaagtttt aaagaattta ggactacaaa
    12361 agctataaaa gatttattag tgagtttaga catagaaata ttagatttag gaatggaaac
    12421 aggagttgtt ggattattaa aagggaagta tgatggtccc acagtagcac taagaggaga
    12481 tattgatgca ttgccaatat atgaagaggt tgatattaag tataaatcca gatatgatgg
    12541 tataatgcat gcatgtgggc atgatataca cacgtcttgc cttgtaggat gtgcatatgt
    12601 attgtctcat attagagata gtcttcatgg aaatgtaaag tttatatttc aaccagccga
    12661 agaagtcaat aaaggtgcga agatgctagt ggaacgagga gtaatggaaa atccaaaagt
    12721 tgatgctata tttggacttc ataatcatcc agatatacct tgtggaaaaa taggtgttaa
    12781 gttaggtggg ttaatggcag cagtagacac tattaagata gaagtaaatg gatttggagg
    12841 tcacggaggt atacccaaca gaacaataga ccctatagta gcatcaagtg caattataat
    12901 gggtattcaa accatagtaa gcagaaacat aagtccatta gaatcagctg ttatatcaat
    12961 aggtactata aatggaggga ctgcaaataa tgtaatttca gaaaaggtag atatgacagg
    13021 cacatgtaga agctttagta atgaagtacg aaagaaaata tcagaaaatt tagaaaatat
    13081 agtttgtgag atagctcgtg gatatcaagc aactgcaaag ctagattatt tatttgattt
    13141 accagctgtt ataaatagta aagagatgta cgcaatagct tgtaagtctg tgtgtgattt
    13201 gtattcagaa gatgctatag ttgatccaat accttctaca ggaggggaag atttttctat
    13261 attcatggaa aaagccccag gattttttta ctggttaggg gtaggcaaca aagaacaaga
    13321 ttgtatatat cagtggcata atcctaaatt taaagctgat aaaaattcta tattggtagg
    13381 tacaaatgta ctatgtcagt ctgttataaa ttatatggat aaattaaaaa ataaaattta
    13441 agatagagca ttctggagat aaagtttatg agggggtatt agtttgagga aaattaattc
    13501 acaggaaaaa aacaaagata gtggattatt gatgagggca ctaaatgttg tagagagagc
    13561 aggaaataaa cttccagacc cagtaaccat atttttatta ctatgtataa ttgttgtaat
    13621 attgtcagca gttatatcta atttaggagt agaagaaata catccttcta caaaagaagt
    13681 tgtaaaggta gtaaatctac tagagaaaga gcagattcaa tcatatttag gtagcatagt
    13741 tacaaacttt cagtcatttg ctcctttagg attagtactg gttacaatgc ttggagcagg
    13801 tgttgctgag aaatctggat ttatggaagt gttaatgaaa aagggtataa gtaaagttcc
    13861 acaaaaactt gtaactgtag caatcgtatt tgcaggtatg ttgtcacata cagcagcaga
    13921 tgtaggattt ataatattac caccacttgc agcacttgtg tttttaggaa ttggaagaca
    13981 tcctcttgta ggtatgtttg cagcttttgc aggtgttgca gggggatttg cagcaaatgt
    14041 tatgcttagt actacagatg tattgttagc tggatttaca ataccagcag ctcaaatgat
    14101 ggacccatct taccaaggca atccagcaat gaatctttat tttgctttta tatcagcgat
    14161 agttttaact tttgcaggag catttgtaac tgaaaaattt atagctcctc gttttacaaa
    14221 atataatgat tcaacaaatg ataaggatat acaggaattg agtgaacttg aaaataaggg
    14281 tataaaatac gctttattat cattgttagt tgtagtagta gtgatagttg ccttatgtat
    14341 aggtgacaat gcatttatga aagatccaga gactggttct atactatcta gtaatgcacc
    14401 tttaatgaaa ggtatagttc ctataatcac tattatattt ttaactccag gattagtgta
    14461 tggaaaagta agtaagaaga taaaaagtga caaagattta gtatctatga tgggtagtag
    14521 tatgagtgat atgggtggat atatagtcct agcatttata gcatcccagt ttattaattt
    14581 atttaatctt agtaacttag gaacaatttt atcaataaca ggagcaaaat tactagcaga
    14641 gtcgggaata cctagctatg gattgataat aggatttata ttgctatctg gatttatcaa
    14701 tttatttgta ggaagtgctt ctgcaaaatg ggcgatttta gcacctatat ttgttcccat
    14761 gtttatgcta ctagacttta atccagcatt aactcaaata gcttatcgta ttggagatgc
    14821 ttcaacaaat ccaataagtc cactttttcc gtattttcca gttatattgg cctttgccag
    14881 aagatatgat aaagatatag gtataggtac tgtcatatcg aatatgattc catactcagt
    14941 ggtatttaca ttaatagaaa taataatatt gttactattc atgggtattg gcataccatt
    15001 aggacctggt ggaggaatat cttacgtttt atagaccgtt ataataaatt tatcaatgaa
    15061 gattaaacta ataaaatata ttctttaatt aagaggagga ttattatgtt aaaaacaaca
    15121 aaaatttcag acccagagtt atacaagatt gtagctgatg agttggttag acaagaacac
    15181 aatattgaga tgatagcttc tgaaagtaca gcaccaacag aagtacttga attaagtgga
    15241 tgtgtattta caaataaaac agaagaaggc tatccagggg ctcgttttca agcaggttct
    15301 gaagaagcag ataaacttga aaccctagca ataaaacgtg caaaagaagt atttggagca
    15361 gaacatgtaa acgtacaacc gtattcaggt tctacagcta actattgtgt atattcatca
    15421 atattaaaac caaatgacac tgttttaagt atgcgtcttg accaaggtgg acacttgaca
    15481 catggtagcg cagttaattt tttacatgac atttataaat atgaatttta tggggtagac
    15541 ccaaatactg gacgtataga ttatgatgct cttgaagcaa aggcaaaaga atgtagacca
    15601 aaacttataa ttgcaggggc aagttcatat ccaagattaa tagattatga aagaatatca
    15661 aaggttgcaa aagaagtagg agcttacttt atggtagata tggcacatgt tgcaggatta
    15721 gtcgcagcga aagtaatacc tagtcctgta ccttatgcag attttgtttc ttcatctacg
    15781 acaaagactt tctgtggacc aagaagtggt attgtacttt gtaaagcaga acatgcaaag
    15841 aaactcgata aaggtgtttt tccaggaaca ttaggttcta tacatcttaa tacagttgca
    15901 gcaaaagcct tttcactatt atatttaagt acagataaat ttaaaaagat aatggagcaa
    15961 gttgtagtaa atgctcagac tctagcttct gaactgattt cacatggttt tagtattgta
    16021 agtggaggta ctgataatca tatagtaatg gttgacttaa gaagtaagaa tcttacagga
    16081 aaacaatttg aaaaagctct tgaatatgta ggaataacag ttaataaaaa tgttatacct
    16141 gacgacccac aatcaccatt tgtaactagt ggtgttcgta taggtcttac atcaatatct
    16201 caaagaggtc tgaaagaaaa agaagttata cagatagcag ggataatgaa taaggtagca
    16261 gaaaatattg ataataaaga agtattagat gaatgtaaag cagaggcaca agaacttata
    16321 tctaagtttc cattatatcc agaagggtat tttgaagatt aaatattttg atttgtatag
    16381 ctcacaattt ataaagcctg taacataaaa tttaataata tataaccact ataaggtgtt
    16441 gattaatcaa caccttattt tttttgaaaa gtaatataca agctatcata agcatataat
    16501 tccttataga taattaacaa gggggataaa gttatgaggt attataataa acctaccaaa
    16561 gaagtattaa aatacttaaa aacaaatcca gaaattggac ttgatgataa tgaggtagaa
    16621 gaaaggaagc ttagatatgg attaaatgaa tttactataa aagaaggtag aacattttgg
    16681 gacgagttgg gagaaagttt gacagaacct atgatattaa ttctcatagg agcagcagta
    16741 ataagttctt ttgtgggaga attacatgac gcattaggta ttttaggggc tatttttatt
    16801 ggaatttcta taggcataat aactgaagga aaatctaaaa aagcagctca tgccctgtca
    16861 aaattaacag aaaatataga agtaaaggtg cttagaaatg ggaagataat taaaatatca
    16921 aaaaatgatt tagtaccagg agatattgta tatatagaaa ctggggatat gataccagca
    16981 gatggaagac ttatacagtc tataaatttg aaacttagag aagatatgct aacaggagaa
    17041 tctgatgatg tagctaagaa tgctgatgct gtattagata tggaagtagt ttattctaaa
    17101 acagaaataa tcgaacagga tgcaatacct gcaaagcaag tcaatatggt atttggaggg
    17161 actcttgtag catatggtag gggcataatg gttgtcactc atactggtga taagacagag
    17221 atgggtaaga tagcacagaa tttatcaaat gaagaccagc aaactccact tcaaattaaa
    17281 ttagggaagt tgggtgctaa aatagcagga atatcaggca taatagctac tttattgtgt
    17341 atgtttatga ttatacagat gcagagaaaa ggtatgttaa ttttagatac aagcagtata
    17401 ttatctttct tacaatcatt agaacctgct aaaaatgcat atatggtttg tattgcatta
    17461 atagttgcaa ctgttccaga aggtttgcca actatgataa atattacctt agctataaca
    17521 atgcaaaaga tggcaaagat aaatgcatta gtaacaaaga aagaagcctg tgaaactata
    17581 ggttctgttt ctgtaatatg ttccgacaaa acagggactt taacacaaaa caaaatgatg
    17641 gtagaggtag catatgttga tggtaaatat ataagtggag gagagtatca aagtaatagc
    17701 tattttgaac aaaactgtat agtaaattca actgcagata tagaaaaaga agataattca
    17761 tttaaatata taggaagcgc aacagagtgt gcattacttt tatatcataa tgataagaac
    17821 tataatgaaa tgagaaaaca aacgtatttg atatcacaaa taccatttag ttcagaagaa
    17881 aagaagatgt caacattaat tcgtcaagag gattcagata tattactttc aaaaggtgct
    17941 ccagaagtat tattaaaaaa atgttcttat gtacaacaag gaaaaaatat tgttccaata
    18001 actccaaaag tagaaaaaag tattttagat gagattaaga agttacaaat taaatctatg
    18061 agaacattag gatttgctta taaaaaaatg agtaattcta aaacagaagt tgcaatgact
    18121 tcagaaggtg aattgaattt aataggaaat tcaagaagtt atatgaagga agataattta
    18181 gtttttagtg gatttgtagg aatagtggac ccattgagag aaggggtcaa agattccatt
    18241 gataaagcat ttaatgcagg agtagatgta aaaatgctta caggagataa tataaataca
    18301 gcaacagcta ttggaaatga attaggatta ttaaatgatg ggaaaaaggc agttgaagca
    18361 acatatatag atgtattgac agataaagaa ttgagagaag aaatcaaagg aatatctatt
    18421 gttgcaagaa gtaaaccaga tacaaaaatg agaatagttt cagcacttca aaaaagtggc
    18481 gaagttgtag ctgtaacagg tgatggtata aatgatgccc cagcacttag tcaagcagat
    18541 gttggaattg caatgggtat ttcaggaaca gaagtatcta aaaatgcagc agatataata
    18601 ttgactgatg atagctttag tactatagta gagggaataa aatggggaag aggtatatat
    18661 gagaactttc aaagatttat acagtttcag cttactgtaa atatagtagc atttataatt
    18721 gctataatat ctcaacttac tggtaaggat atgccattta ctacaataca attattatgg
    18781 gtaaatataa taatggatgg tcctccagcc ctagcattag gattagaacc tgttagagat
    18841 tatgtattga aaagaaaacc aataaataga cattctggaa taatagctag gtctatgttt
    18901 gtaaatatta ttataaatgc aattcttata attacaattg tatttactca atcagctttt
    18961 aatatattgg gagctacatc agaagaacaa ggtactgtta tattttcttt atttgcattc
    19021 agtgcccttt ttaatgcatt aaattgtaga gagtttggtt taaatagtac aataccaaat
    19081 ttctttaaaa ataaactggc attgcaaata atagtagtta cagggataat tcaaataata
    19141 tttacacaag tgttccagag tttctttaat tctgtttctt tggactttga tatgtggata
    19201 aagataatac tatttgcatc cactatccta ttgtcaaatg aatttgtaaa gctaattctt
    19261 agaacaatga aaaatagtag aactatgaac tttaatagta aaaattaatt cttatgaaac
    19321 cgtaaagtac tatatattgt aactttgcgg tttttgtttg tttaatgttg ttttatatta
    19381 ccattttcat atttaatttt ttattaatct gaacacaatc ttccattgta attgcttcat
    19441 ctgtgtcaac acattcaaag tcaatatcta atttttgtaa tattttatat gcctcttttt
    19501 gcaaaggagt atcaaattta ataggtgatt ttgtgtaaat atcactgata aaaaacatga
    19561 tttatttcat tcctttccat ttaaaatctt atagttatag tgtatcataa ggttataaaa
    19621 tatagataaa tatagtataa atttaaaata tgtacagaca aaacaaaaaa atcatattct
    19681 aagtaatact tgggaaaaat gatattatca taataaataa tctttgtaaa aacttatata
    19741 gaatctatta aacagacact aagccataaa aactttacta aatataaaat tcgtatctaa
    19801 atagatatat agaataatat tttactgaag tagagtgatt tttaagaatt ttatatataa
    19861 attatgttat cataaataaa gatatagatt ataaaatttg ttaataaaat aaagggcagg
    19921 gaggtaagtt tcatgttttc caaaatccta gtcaaaacat ttatcagaga tagcgaaaac
    19981 gttcaaaata cagatgttag aaataagtat ggttatgtag ctggtgtagt aggtatatta
    20041 tccaatttac ttttatttgt gataaaggta ttcataggaa tgctcacatc tagtatagct
    20101 ataatggctg atgcatttaa taatctttct gatatggcat cttcagcaat aactatgata
    20161 ggttttaaat tagcaagtaa gccagctgat aaagaacacc cttttggtca tggaagaata
    20221 gagtatttat cagcattaat agtagcattt atggtgatgt tggttggact tcaatttgta
    20281 aagtcatcta tagaaaggat agtaaatcca attcctgtaa aatttgaagt aataccactt
    20341 atacttttaa ttgcatctat aatgattaaa atctggttga gtcgttttaa taaatttatg
    20401 ggaaataaaa ttgattcttc agccttaaaa gcagtttcac ttgatgcttt aggagatgta
    20461 tttacttcaa gttgtgtagt tatatcattt attgtagcta gatttactaa ttttcccata
    20521 gatggatatg ttggtatcgt agtatcatta gtgatacttt atgctggttt ctctcttgta
    20581 aaagatacta taaatcctct tttaggggaa gcaccggatg aagaaatggt aaattctatt
    20641 atagagctat tgttatctta taaatatata ataggtacac atgatttaat tattcataac
    20701 tatggagttg gtagatgtat tgcatctata catgcagaaa ttccatccaa catagatata
    20761 atggaaatac atgaaattat agatactgca gagagagaaa tatctgaaaa attagatata
    20821 tatctagtaa ttcacatgga cccaatatgt cttgaagata aagaagttat gtcagccaaa
    20881 aaagaacttg aagaaatact taagaaaaat agtcttgtaa aatctatgca tgattttaga
    20941 atagttggaa aaggaagcaa gaagaattta atatttgata tagtagttaa tccttccgaa
    21001 ttttccaaaa acatgtcaga agatgactta aaagaagata taacaaaatt agttaaggag
    21061 ataaatcctg aatataattg tgtaattgtt gtagataagg attttattta attattaatt
    21121 aatctcatca taaggtaaaa aatgtaaaaa ggatagacaa tagtatagag gcataatata
    21181 atagaagtat ataattgtat ttttaatata aataaagcaa ttttaattgt ttttatgaaa
    21241 tattatagag atgttgaagt atattctatt attgggcacc ttatggatat actgagtcag
    21301 tggtgcaacc ggctatgaat ataaatttat ttattttcat agcttttttt gtataatttt
    21361 ttagtaaaaa ttactttata tacgatatta atggaagatt agattataaa ttataggtaa
    21421 cctaaagggg tgagatttac atgaagaaag gaaatagaaa ggcattatta attagtttaa
    21481 ttatgatttt aagtatggtg gtgtctacaa tatatccaac tgtatcttat gcttcggaat
    21541 taggagagaa tagtcagatt caaagtggtt caactaattc atcaactgga gaggagaagg
    21601 aaagcgacaa taaaaagcca gaacagactc cagagaaaga taaggcaaca gataataaaa
    21661 aaccagaaca gaccccagag gaagaaaaac caacagataa taaaaaacca gaacagaccc
    21721 cagaagaaga taagtcaaca gataataaaa agccagagca aactccagaa ggagaaaagc
    21781 caatagataa taaaaagcca gagcagatac cagaagaaga taagtcaaca gataataaaa
    21841 agtcagaaca agctttagaa gatgaaaaac cactagacaa taaaaataca gaaaaaactc
    21901 cagaggaaga taatctatta gaaaatgaaa atttattgaa agttttagaa gaagaactta
    21961 atgaagaaaa tgaagactat ggatttgttg taaaaattaa taacaataca atagaaacgg
    22021 aatcaatgaa aaaaatatca tttaatttaa cttatactcc aacatcaaaa ggaatacaag
    22081 caggagattc aataactttt aaggtgccag atgtattcaa taaagttaat ttagattata
    22141 caagtgaatg ctttgataaa acagaaagta atggagagta tacacttaca tttagagaat
    22201 tacctaatgg ccaaagtgta atgcaaggta aaataggttt ggaagcatat gttaaaaagg
    22261 ttgatgaaga tactaatgca aaaatacata ttgaaactac tggaaaaata gaaagtggaa
    22321 gtggagatat agatgtagaa ataaaacctg gagataaaac ggatgttcca gatgctaaag
    22381 gaacacttaa aaagcttgtt gaaggaaaaa aatcaactac tgtatttatg ccagttaaaa
    22441 ataaagatat aaattacagt atacaagtaa atgaaaagca ggaagagtta aaagatatta
    22501 ttttgtatga tgagcttcct gaaggattga cattgataaa tggttctgta agtgtagtaa
    22561 caagtgatgg aaaagaagtt agtgatttta atattgaaca gagtaaaaat tctatttcag
    22621 ttaatcttgg aaatatagat aaatcgtata ctgttaagta taaggctaga atcagtgaca
    22681 aaaatgcaaa acatggtaat aaatataaga atgtagctag gattgaatct gatggtaaga
    22741 agatacaaga agatgatgct actgtaagta tatttgatag aggagatgat tatcttctta
    22801 ctaaaggaca ttcaggagca acaaatatta ctcaagttgg tcaagtaata aactatcaga
    22861 tttcaataaa tgatgataaa tcaccaataa gtaatgttgt tataacagat aatataccag
    22921 aaggcatgag attaactact tcaggagaag ctgggcatga ttttagagtt gtagaaattc
    22981 caatgaatgg gagctggact ccttggagta aagaaaaaat tgccaataat atttcatata
    23041 aagtagaaga aaaaagaaat gaaagtgggc aagtagataa agttataact ggatttacaa
    23101 ttaatctcag taaagaagaa gtggaatcaa agttttttat agcttataca ttaaaggtaa
    23161 ttagtattga agatagctat ataaatagag ctgttcttga tgcaaataat agtgaaattg
    23221 ataaaaatga tgagataaac tttaagaaaa attctggatt aataagcgca aaaaaagaag
    23281 ttgataaaaa agttttaaat agtagtgata accagattgt taaatacaaa atcaatatgt
    23341 caacttatgg ggtttatgat gcaggacaag taaatctttt agatgaagta aactcagctc
    23401 tagaaatatc taatataaaa tattctgata atcttgaact taaaaaagaa gctggagatg
    23461 gaaaaaatac aataagatta gtaaataaat acgaatttaa acagataaaa gaaggggaac
    23521 ctgtgcaaag ttgggttaca tttgatgcag acttcacaaa tgtaaaagtt ggggagacta
    23581 tacgaaatgt ggctcagatt aatggttcaa gtcctccagg agtagagact actaagcaag
    23641 gttatgcttt tgaagctaaa aaagtggatg ctttagataa aaatgtactt tcaggtgcta
    23701 agttcaattt agaagatgca tttggaaata tagtagttaa ggatttagtt agtgatgaag
    23761 atggtataat tcaatctagt ataaaaaaac caggaacata ttatttagtg gagataatgg
    23821 ctccaagtgg atatgaaaaa ctgaaggata aaattaaggt tgaaattggt aataaagata
    23881 ttggtagaat agtggatatt ggaaatatag aaaacataag acaagaaaat ccaccagtaa
    23941 atccaccagc accaccaaat acagatgaac caatagtaaa tccaccagta ccaccaagca
    24001 cagataaacc aaggaaacca tcaagttcat ctgatacaga ggatactata gttataaatc
    24061 caccaatacc accatcagaa gatataataa atccgccaat accagaggta ttgaatccgc
    24121 cagtaccacc atcagaagag atgatagaaa ctcctgttaa acagattatt ccaataccag
    24181 aagtagtaaa gccatcagtg tcagaagaaa aaaataataa agttaaagat gatactttgg
    24241 taaatccacc agtaccacca aaaacagggg atagtactac aattattgga gaaatacttt
    24301 tggttatagg agctattgta ggacttatcg tattaagaag aaataaaaat acaaattaga
    24361 ccctaacatt ataaaaaata taagttatat taaaaaatat cttctaaaac aatatttaat
    24421 aatgaaaata ttgttagaag atattttttt ttgaagattt atgtaaaaaa ataataactt
    24481 tttagaaaaa agttccaaag ggatatagac aattaaaaag atataagcta taatatagat
    24541 ataaaattaa atgaggcaaa actagggaaa cctagtcacg caaagctata ggggctaaag
    24601 tttagtaaac catgccagcc agttgccgaa aagagtaaaa atttcttttt gtatatacaa
    24661 agatactctt gggtatcttt tttattttga aatttattac tcccggccta actcaaagga
    24721 gggaataata tgagaccaag taaaaaatta ttaatagcta taatatcaat attcttaata
    24781 agttcagttc cagtaagtgc acatgcagat agtactacta tacaacaaaa taaagacaca
    24841 cttagtcaaa tagtagtttt tccaactgga aattacgata aaaacgaagc taatgctatg
    24901 gtaaatagat tagctaatat agatggaaag tatttaaatg cattaaagca aaataactta
    24961 aaaataaaat tattaagtgg aaagttaaca gatgaaaaag aatatgctta tttaaaaggt
    25021 gtagttccta agggatggga aggtactgga aaaacttggg atgatgttcc aggtttaggt
    25081 ggaagtacag tagctcttag aataggattt agtaacaaag gtaaagggca tgatgcaata
    25141 aatttagaat tacatgaaac agcacatgca atagaccata tagtattaaa tgatatatct
    25201 aaatcagcac aattcaaaca aatatttgct aaagaaggtc gttctttagg taatgtaaac
    25261 tacttaggtg tatatccaga agaatttttt gcagaatcat ttgcatatta ctatttaaat
    25321 caagatacaa atagtaagtt aaaatcagct tgtccacaaa cttatagctt tttgcaaaat
    25381 ttagctaaat agagttttaa taaatttaaa ataaatataa ataaagagat gctattataa
    25441 tagcatctct tttatattgc gtttaaatct aataaatttt gtcttatgta taactatgat
    25501 agattatcaa ttactactcc ttatacaagg cttcaacgtg tttattatgc atttgagtaa
    25561 ttatcgtagg tataatagaa attattgaac caattactat caataaaatt cctataacca
    25621 tgtttatagg gataatttct ttaagtatta ataatgctag tattggagat attactggtt
    25681 taaagaagaa tactaaagat gtaagactag tagatgtagc ttccattgcc ataaagtaaa
    25741 gcgcataacc aacacctgta acaaatacga atacataaat cataatgggc atattgtgta
    25801 aggtatatcc actaaaaata gggatatttg caaatacatg aagcttattt ttatttaata
    25861 tatctgcgat aaaattaatt ttagtgagta aaaccaatat caacatttcc aaactaccaa
    25921 atataaagct aaagcaggtt acaactattc caccaaattt taaggttttt cttttaccaa
    25981 atacagtata cattgaaaaa ataatagcag aagataatgt taagaatatt ccactaagag
    26041 ttagttttgt atgcaatgga tttattataa ctataattcc aagaacttct aatataagtg
    26101 aaattacatt gtgcctatat attttttcct tcaaaattaa gtaagctaaa agcattacaa
    26161 agactggatt acaactgaac aaaacagcaa ctacagatgc ctttgtgttt agaacagcaa
    26221 gttgatacaa tgacatactt actactacac acataaaacc taaaaatgca aagtatttta
    26281 agtctgattt tgtaatagaa agactaagag ctcttaatct ttttacagca aaaggtagta
    26341 agactaaacc gccaacaaaa aatcttgata aagttatttg taagggatta aagtcattag
    26401 tgacagtttt tagactaatt tccattgaac taaaaaataa ggtagtcagt attatataaa
    26461 cataaccttt tttcaagtca aacacctcct tgtaatttat attagtacta tagataagaa
    26521 tattcaagca tattggaata atatggtatg tataaaatat agtacataaa attataaact
    26581 accttaatat agaatgtaag tattctatac taaggtagtt aattatttga attactttga
    26641 tttagttatt ttaaattata taatttggct aatttttcaa atgctggtaa agaactttta
    26701 actttatcat aagatacaca atatgatact ctaaaatgac ctggacaacc aaaagaagaa
    26761 cctggtacta ataataaatt aaattgtttt gcatcattgc agaatttttt atcatcttca
    26821 attggagctt ttgggaacaa atagaatgta ccattaggtt taacacaaga atatcccata
    26881 tcgattaaac tattatatag aaggtcaaaa ttctttttat aaatatttac atcaacttct
    26941 gcatctaaac ttcttgcaat aaccctttga aaaagagaag gagcatttac aaatccaagt
    27001 attctatttg caacatttaa tgaagacata acatcatcaa aatcatccat ttgcccatta
    27061 gcaacaatat atcctatacg ttcaccaggt agagataatg atttactata agagtatcct
    27121 ataaatgaat ttctatagta ttttagtaca caaggaactt tggcaccatc ataaattatt
    27181 tctctatatg gctcatcaga aattaggtat atgcttgtat taaattcttt ttgttttgaa
    27241 tccaataatt ctccaagatt ttttaataat tcttcagaat atatgacacc agtaggatta
    27301 tttggagtgt ttattattaa agctcttgtt tttggagtaa tagcattttt tagagcatct
    27361 aaatctggtt caaaagtctc tatatttgta ggaacttcta ttaattttcc atcatagttt
    27421 tctgtataat ttttatactc accaaaatac ggtgcaaaag caataacttc atcaccagga
    27481 tttaaaagag tttttaaaat aatatttaga ccaccagcag caccacaagt cataattaaa
    27541 ttttctcttg taagatttaa tccatctttt ttatttatgt gttcagcaat agcatctctt
    27601 acatcctcat atcctgagtt gttcatatat ccatgaacta aatttggaga ttcttcattt
    27661 aaaatatcat ttataactgc tttaatagtc tctggaggtt caacacttgg atttcctata
    27721 ctaaaatcaa aaacattttc ctcaccataa atatcagaaa gtttctttcc ttcttcaaac
    27781 atagctctta taacagaact atttgcaact aaagtttgca ttttattaga tatcatatgt
    27841 aacactcctt tttatagatt tatattgctt atgtatatat taccatattt ttactgaatt
    27901 tagacaattt tcgaaaaatt acttgtttga aaatataaac tttt
//
LOCUS       DAMJVK010000049        27148 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.049, whole genome shotgun sequence.
ACCESSION   DAMJVK010000049 DAMJVK010000000
VERSION     DAMJVK010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27148
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.049"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(39..899)
                     /locus_tag="PC414_002152"
     CDS             complement(39..899)
                     /locus_tag="PC414_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HDF2464547.1"
                     /translation="MAMKWRDYADDVNLFEDFDKTIKPLTDEQRRKNINTLIAYFSKE
                     VPSKIYNLSNDEIKPRDILRGLLNVYPPKEIAPEILNMLHNLLLIECEERELVDVNDI
                     EEIEEGIAIWRGNITNLRADAIVNAANNKLLGCLQPLHLCVDNEIHSCAGPRLREDCD
                     KIIKKQGHLEYTGDAKITRGYCLPAKFVVHTVGPIVSGGQPSKEQEKQLLHCYKSCLN
                     TIKEIDEIKNIVFCGISTGVFGYPKKEAANLAVSRVRLWLKENPEKNLKVVFNVFTEE
                     EEEKYRRIFK"
     gene            complement(1216..2544)
                     /locus_tag="PC414_002153"
     CDS             complement(1216..2544)
                     /locus_tag="PC414_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HDF2464548.1"
                     /translation="MEKFSVVIAGGGSTYTPEIILMLLDNLDRLPLRAIKLYDNDEER
                     QNKVAKACEILIKEKDPNIEYLATTCPKEAYTDVDFCLAHIRVGKLEMRELDEKIPLK
                     HGVVGQETCGPGGIAYGMRSIEGVLENIDYMEKYSPNCWMLNYSNPASIIAEAVRRLR
                     PNSRVINICDMPIGMEHNIARIAGLKSRKHMDIRYFGLNHFGWYTSIKDKKGNELLPK
                     LVEHIKEYGFINGEEGMKNDKKDSWFETNLFTKEIVKTDLTTIPSSYLKYYLFPDYVV
                     NHSDVNYTRANEVIDGREKEVFGSCALIEKQGHSKGSGLKIGIHAEFIVDLATALAFN
                     TQERMLLIVQNNGAIENIQDDAMVEIPCIVGKDGYEPLSIGKIPTFQKGLIEQQLAVE
                     KLVVDAWIEKSYNKLWQALMLSKTVPSATVAKELLDDLIEANKNYWPKLN"
     gene            complement(2692..3021)
                     /locus_tag="PC414_002154"
     CDS             complement(2692..3021)
                     /locus_tag="PC414_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF2464549.1"
                     /translation="MVPEMEEKVFSIISEAGDAKSDVMMSLKEIKKGDYNKAKHLLNS
                     ASEKIQTASKHHLELLSNAMNSEDSGTDFLVVHSEDHYSNALFAHSLVSELVDIFEMM
                     DPRINKN"
     gene            complement(3045..4334)
                     /locus_tag="PC414_002155"
     CDS             complement(3045..4334)
                     /locus_tag="PC414_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2464550.1"
                     /translation="MEALQNNLRRFLLPIAQKIEKQRHLQAIKEGMISITPIIIVGSL
                     SMLFMALNNMLPEGSAKTLLSENMDTLLIPNKFTMSLLSIYSAFFIAQALAKKYNLNH
                     VEIGMTAVVAQLVVCGQVVDGVLDTSYLDAQGLFVSILVALLVVDITKFMNDKNLVIR
                     FPKEVPSVVNKSFRNLTPMIVCIMLFTAIAAITKNVSGQPLPAIIMNFLAPAISSVDN
                     VFAVTIILFITQLLWFFGLHGAAITSSIWMPIAATYMAENATLIAAGGDPKYVFTIGF
                     YYGFLQVTGSGITLGLVYLMSRSKCKSFNSMGKVVILPSLFGINEPVIFGTPIVMNPY
                     MFVPFVFGPVLVGALNFMALKVGLVGLPIAEPPGFLPPGVAAFLMTLDWKAIVLVFAS
                     IILMTLIYYPFFKIMEKEELNKNAELATTLDDDSFDF"
     gene            complement(4350..4676)
                     /locus_tag="PC414_002156"
     CDS             complement(4350..4676)
                     /locus_tag="PC414_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2464551.1"
                     /translation="MNILLVCANGASTGVLVEKMKSFCSEHEKLKTKTINIEATSFEN
                     LKSYIEANDTDVVLVAPQIRFKEDDVVEACKNYKIAVGLIDTKHYGRMDAPSVMKSAI
                     ELYKNR"
     gene            complement(4811..5440)
                     /locus_tag="PC414_002157"
     CDS             complement(4811..5440)
                     /locus_tag="PC414_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464552.1"
                     /translation="MQDKILRDKKANDPWARMTTRNGFSADEIISSFQKAIRRNMVEE
                     ACEFAYELYISSPELEDKLWRRILTISVEDIGMGDPSAVIIINNLNQVRKEYSYADGD
                     RPLFFIHAIRYLCACEKDRSSDLLKNIIIKGFAMGKVPEVMDVALDKHTYRGLEMGRD
                     SFHFLNEASIVIPEKEVDNDYKERYLKILEKYKQEEVIDTAFKFNHWQY"
     gene            complement(5655..8312)
                     /locus_tag="PC414_002158"
     CDS             complement(5655..8312)
                     /locus_tag="PC414_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2464553.1"
                     /translation="MIYKKDKVMKVLESSYRENEKGITANQLSEKTGIARNNISTYLN
                     QLFNEGMVTKIKGRPVYYIPANLNLNKNELLDTQSELLGIKKGTKVENKIKKDSFETL
                     IGKEHSLRPIIEKCKVAMLYPTNGLHTIIYGETGVGKSMIARYMYDYSIDSGIRKNKA
                     PFVTFNCADYANNAQLLMGHIFGVEQGAYTGAESSRKGLLEIANGGILFLDEIHRLPA
                     EGQEMLFTFIDKGTFKRLGDATKERESKVLIICATTENPTSTLLDTFNRRIPMKIEIP
                     SLRDRTMIERMELVKNFFKEESKSISISIKVHKEIIKSLLLYDCKNNVGQLQNDIKLA
                     VANGYLRYKRDNKEPVYIEKKYFDKSINEVSDDYREKSIKADEVITHGIEYFVFTSLG
                     EEEVINYNDINIINAVNTNLNKSVEMNIADASLFNNVKFKSMCESIRQIIKEDEEIDI
                     SESIFKSMAIYIKSVLDKSSSTNNIDLNQIRRNNRSEFKTALKIVGIIENEFDLFLSI
                     ETVAYITLFIVQAKQDEQNKIVNNINIIVAMHGETTASSMVKAVGDIIGKCSAISFDM
                     KLDKSYDEVIVDFKNLINNIDNKDVLLFTDMGSLNSFDEIIKKEKKCGVRVIPMVTTL
                     TVLEAVQKANMGLPLNDVYNSITNTRKYYFGTNEIQNKENLSKTIIIASHVSEGVDNK
                     TRKILEEKMSRYLDGIDIISVPYKTEKDLSLNITKLKESSNIVAVINEQRINIRGIDY
                     ISKKDIDKDENINKLKNIIKISIGYDDVVEGLKTSLKSSNYNRIFKDIKYVSDELFLV
                     FNIEKKYDKVIGLMMHLAFMVDGLIGNTREIEKLDKEKTLDYHKSLSKIKDIVSQLDK
                     KYNIEINEKECYQILLILEYAEIIEKDYQ"
     gene            complement(8671..9615)
                     /locus_tag="PC414_002159"
     CDS             complement(8671..9615)
                     /locus_tag="PC414_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2464554.1"
                     /translation="MNREKILEIKNLKQYFHLDKSTTVKAVDDISFDIYKGEIFGLVG
                     ESGSGKSTTGKTIIRLHESTGGEVIYKGNCISDKKTYKFIKKDVNKSMQIIFQDSTSS
                     LNPRMTIADIISEPLKIQGICKNKTDRMNKVYEMLKLVGLDRSYANKYPSDFSGGQRQ
                     RIGIARALSVDPEFIIADEPIASLDVSIQAQIVNLFKKLQQEKNLTCLFIAHDLSMVR
                     HISDRIGVMYNGKLVELADSNELYNNPIHPYTKSLLSAIPVPDPRYAKSRNRIEYNSN
                     GYDCSNEKSLSWIDVSDGHFVYSSKSEINKYQQNLKVV"
     gene            complement(9608..10633)
                     /locus_tag="PC414_002160"
     CDS             complement(9608..10633)
                     /locus_tag="PC414_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2464555.1"
                     /translation="MEHLLEVNNLSVSFKVEEGEVQAVRNVSFNLKKGETLAIVGESG
                     CGKSVLCKSLMRILPYNGYIKNGEVLLKSSDLVKKSEKEMEDIRGKNISMIFQDPMTS
                     LNPTISIGKQIAEAVIIHQGISKSEAKKRAIELIELVGIDNPEKRFKQFPHHFSGGMR
                     QRIVIAIALACNPDVLIADEPTTALDVTIQAQIIDLIKDLQHKIGLSIIFITHDLGVV
                     ATIADRIAVMYAGKIVEIGTVEDIFYDPRHPYTWGLLGSLPTLDSQDDYLYNIPGMPP
                     NLLNPPKGDAFAIRNKNALKIDYEKEPPMFKINDTHSAATWLLHPDAPEVDVPVRVNC
                     GRVISNE"
     gene            complement(10708..12276)
                     /locus_tag="PC414_002161"
     CDS             complement(10708..12276)
                     /locus_tag="PC414_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2464556.1"
                     /translation="MKLKKLKVLSLVMILSLMAGCSSGGDKDKKADTPKDGKVLVYGS
                     NDYTSINPALYEHGEINSLIFNGLTAHDENNKVVPCLAKDWKFDEATNTYTFNLRDDV
                     KWHDGEKFTANDVKFTIETIMNPDNASEIASNYEDITKIDVVNDNTIKITLKAPNTAM
                     LDYLTVGVLPKHALEGKDIATDEFNQKPIGTGPFKLEKWDKGQSITLVKNSDYFVKEP
                     GLDKVVFKIVPDDKAKAMQLKSGELDLAQITPKDMSNFEKDEKNFKVNIMKTADYRGI
                     LYNFNSKFFKDKKAKGLPNALSYAIDRKAIVDSVLLGHGVPAYSPLQMGPYNNPDIEK
                     FEYNPEKAKQEIEKLGWKLGSDGIYEKEGTKLAFEITAGESDQVRVDMAKICAQQLKE
                     IGVDAKAVVVTETDWANQDAHLIGWGSPFDPDDHTYKVFGTDKGANYSAYSNPTIDKI
                     LQKARETEDKDEKLKLYKQFQVEMTKDMPYTFIAYIDAIYVGKPNIKGLTPDTVLGHH
                     GVGIFWNIADWTIE"
     gene            complement(12389..13300)
                     /locus_tag="PC414_002162"
     CDS             complement(12389..13300)
                     /locus_tag="PC414_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2464557.1"
                     /translation="MKPQYSDFEIVGENYILVAEDEHIETKKTLSKKIKELPYISIII
                     LSIIVIGSVFSSFIMTHEPTYMDLASSNLAPNKTFLFGTDSMGRDIYSMIWYGGKISL
                     FIGLFSTVISTVIGIVYGSISGSASELVDDAMMRFTEIILSIPSILIIIFVQAILGNS
                     NPVSMSIVIGITSWMNISKIVRTEVRQIKNSEYILAAKSMGGGFFYVLKQHLLPNFVA
                     SIMFMVVTNIGAAIGTESTLSFLGIGLPIEIVSWGSMLSLSEEALLSNRWWIILIPGI
                     FLVTTLVCITNIGNYIRKNNNKKSSNL"
     gene            complement(13284..14270)
                     /locus_tag="PC414_002163"
     CDS             complement(13284..14270)
                     /locus_tag="PC414_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2464558.1"
                     /translation="MLKDILKKLLQFILVMFLLSFIVFYMARLAPGDPLISYYGDGVE
                     RMSTQEKENAMKKLGLDEPIYSQYIKWISNASKGEFGISFKYKQNVTSVINDVYINTI
                     ILGGSGYILTFVLALLLGIFCTLHEDKLIDRIICKLGTITNCIPSFWVALVLILIFSI
                     NLSILPSSGAYSMGEESSILSRISHLILPLTVLILSHLWYYTYMIRNKLLEEIREDYV
                     LLCKAKGLNNRTIVFKHCLRNIMPSYISIMAISIPHILGGTYVVEKVFSYPGLGTLCF
                     ESAKYHDYNMLLVLCLITGALVVFGNMLAQIINNKIDPRMKYDRGDTNEATI"
     gene            14630..15031
                     /locus_tag="PC414_002164"
     CDS             14630..15031
                     /locus_tag="PC414_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF2464559.1"
                     /translation="MLLKELYSKTMYTVEENFKENGLTHQQIIVIKLIAHNQELTISQ
                     LCDEMSLAKGTVSGIISRLEQIGYIEKFKKSNDKRNTYVKFTTTGFEFATNFKIKMQE
                     SFDDIFKNCDENELSDLVKNLRNILAKVKER"
     gene            15034..15432
                     /locus_tag="PC414_002165"
     CDS             15034..15432
                     /locus_tag="PC414_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03987 family protein"
                     /protein_id="HDF2464560.1"
                     /translation="MNYKLILAIVFITSALIFYTIGVFGERKAKILKKKHVIIFWLGF
                     IFDTLGTFTMSNIANSHTFEVKSALSQNLHSITGLLAIVLMLFHASWATFVLYKDDEE
                     KKKFFHKFSIVVWTIWLVPYFIGMFIGMAG"
     gene            complement(15882..16106)
                     /locus_tag="PC414_002166"
     CDS             complement(15882..16106)
                     /locus_tag="PC414_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HDF2464561.1"
                     /translation="MREKVEKVLEEKIKPVLQRDGGDVELIDVNENGVVLVRLQGACS
                     GCPGVTMTIKAIIENVLVSEVPGVTQVLGV"
     gene            complement(16141..17508)
                     /locus_tag="PC414_002167"
     CDS             complement(16141..17508)
                     /locus_tag="PC414_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2464562.1"
                     /translation="MKEYIINNVEEIREELIDLSKKIWENPELAFEEKYASSIQKEYL
                     KSKGFKIEEVENLPTGFIASFGEGKPVIGILGEYDALPELSQCVSAERKPLVEGKAGH
                     GCGHNLLGVAGVGAVVSIKKLIEENKFNGTIKYFGCPAEEEGGGKTVMCINGCFDDVD
                     CAFTWHPFDINAPWRGGSLANLSVKFKFKGITAHAAQAPHNGRSALDAVEIMNVGANY
                     LREHVIDSIRMHYVITNGGGRPNVVPGFAESWYFIRGKKAKDAEHVLDRLIKVAQGAA
                     MMTETEMEYKVTDGIYDYIPNQCLTDLVYDNMVFVGCPKNTPEEEEFAKKLCDTLTRE
                     ERLGVATSLQNDKSIVESYIHKEIVDGDKDKGLAGSTDVGDVSYVIPVAQFAMAAWPV
                     GIASHTWQSCSSAGSNIGFSAMINSAKVLACSAYDVFMDTKIIDEAKIEFDKSLDGQK
                     FKPLV"
     gene            complement(17592..19127)
                     /locus_tag="PC414_002168"
     CDS             complement(17592..19127)
                     /locus_tag="PC414_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464563.1"
                     /translation="MLNNLSNININENNLIKNQYSISLLKECLNCKVIDEREVYNIQQ
                     EVSLILMDLIKKYTNGQSTSVKTEVAEKLLISIWYAIDAYINKLGDIEKRVVELTAKN
                     VKKVYLGGIEILEEELVRLKDFYKLMMSRKLDTNSIAYNDTLMELSSFFKSYIVKFEA
                     QDVPVSIDYPLALDDMDVKGIYYVKNYIESIDIENRFCNLFKKKDREKLFYDYGMTYK
                     IDCDVILVNLFELIMNNCIFSTILGNEVTNLEISKYEYEFLEKQFKNMYNNITEDKVI
                     DSEESNIIELIEVDDSFNTEVDSNLDYNDFEEINREIRINKKITLLLMEAIEVLIKRL
                     SIEDEDVIRYIKKYEKIFIESVLSSIKSNTFKSMVTVSKNKKEHLEDYIVDDESKLDD
                     ESFREIFNEILDSTSIIEKIRIIKENINAKKDFIDILESECLFGEEYLMLFASLSELE
                     LAILGSVVFYEDIRMREINILEFMLNEKTETTLWKIEYIKFMKRQSEDKIYSIEEHMN
                     KIN"
     gene            complement(19150..19584)
                     /locus_tag="PC414_002169"
     CDS             complement(19150..19584)
                     /locus_tag="PC414_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464564.1"
                     /translation="MISPIILSSINQNLKEIERNELLETNIESGDYGLALSESDVKDI
                     INSRDNTLKGYGRIELDIKVTKQLIENIYTSQYTNVDNYLEAINDMQEIFYYLKNETD
                     DKICDDEIIEILGEFYEKFSGNMDNVRGEADEFAKKFKFGEV"
     gene            complement(19844..20755)
                     /locus_tag="PC414_002170"
     CDS             complement(19844..20755)
                     /locus_tag="PC414_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HDF2464565.1"
                     /translation="MNKKIELIGNFASDWIRYSDYEYKITEEDELYIVPTEDAVFTMY
                     NPFDVADDIIVDIIRIGQEALQDISKETKEMKLKKEVLEFVKKYGLLGLIYSSVYNRD
                     IVGEERVLMIEKNYITKENILSTDEYVNMFIPFASDEDVYFKKYRRGIDVSKREESPK
                     FFGKRPLILDLVFSKFYTEQLDWIVSFAKDMCKHFNQICVYKNTSNLLTESVTIMSGG
                     FNPQKIGFSISQLDKPIIAWEFDSLKTAIETIYAFAITSEPSSLNKCKYCGKIYIPTN
                     SKSQYCSPSCRNCSNVKKSRSRAKKDK"
     gene            21051..21404
                     /locus_tag="PC414_002171"
     CDS             21051..21404
                     /locus_tag="PC414_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDF2464566.1"
                     /translation="MDRKRELKQLYKEMKFDTGVFIIKNDITKKIYLGKSNDIKSKFN
                     SLKFQLGAGSCMIKELNNEWKKYGEKAFTFEVLELIKHDDNKTEKDYLEELDILEMVW
                     LEKLKEDNTYEIYSI"
     gene            complement(21623..22564)
                     /locus_tag="PC414_002172"
     CDS             complement(21623..22564)
                     /locus_tag="PC414_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp25"
                     /protein_id="HDF2464567.1"
                     /translation="MRISKKVLALGISALLLSVSFPTSINALDKIENIQGVDKYETAG
                     LISDKQDFTTAVLINADSTMVDGIAASGLAGVNNAAILLTNKDDIPEATLQRLNRVTK
                     IYVIGGENSISKDVEKMLLMRRMQVIRIDGVDRVDTSYKIAGEIEKIKNSDKMFLVNG
                     FKDEADAVSVASVAYRDGAPIILTKDIPSAEEDTDLDPWFGVSPVPVYAIGGESTLSD
                     YIVSRYRATRIGGVDRYQTNKNVIEKFYNGAKEFYITSGDDLVYALVASPLAKNAPVV
                     LVSNKSDKSILSGASKVTAIGISDKSIIEQCLDAVKK"
     gene            complement(22665..23900)
                     /locus_tag="PC414_002173"
     CDS             complement(22665..23900)
                     /locus_tag="PC414_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDF2464568.1"
                     /translation="MKILHIITQKPNSTGSGIYLSGMIKGFEKIGHKQAVIAGIDVND
                     DVNCFPSEVSFYPVKYNCGELNFPVVGMSDSMPYESTRYKDLNIDMINRLKYQFKVNI
                     DKAMNDFKPELIICHHLYLLTAFVREMVKDIKVMSICHGTCLRQLNTIDLEKEYIIAN
                     IRKLDLIFALHENQKYDIIKTFGVSESKVVVIGSGYNDDIFYNKNYKIKDDKIKIVFA
                     GKICKSKGLIPFIKAISKLKYSKDLIEVNFAGTGSDIESYNEIVKLASKSPFKMNFLG
                     KLEQRDLAELFNRSQIFVLPSFYEGLPVVVLEALSCGTDVITTDILGVKEWIGSEINN
                     SGKIEYVSLPFMEKEGIPKDEELYDFENNLYNAIDSKIQSLLNNSNKKTSVDMSKKTW
                     DGLAYRINEVILMDELCLV"
     gene            complement(23944..24798)
                     /locus_tag="PC414_002174"
     CDS             complement(23944..24798)
                     /locus_tag="PC414_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464569.1"
                     /translation="MALNIRNYSLLCNQGTAKFNEDVVGINPFGAWVLDGATGLNGKN
                     LVSTESDARWYVQWWNRYLYKNINKEESLKSIINTGIIEVKKEYELRLNGFKVEKLDL
                     PSSSIVVVKFHENKVEYFLLGDCALFIKNGDSKVIKDRSICKFDNTVFDEMSKLSNLE
                     EMAFDEIRTSVMDTIVSNRLKKNTKEGYWILDFDEEAVENSIYGYIEIKKDFQLMLTS
                     DGFTSACDRYNLIKEEELIRIAEKFGIGYIHNKVRDFEDNDYKAVKVPRFKVKDDSSC
                     LYLDIYND"
     gene            complement(24926..26101)
                     /locus_tag="PC414_002175"
     CDS             complement(24926..26101)
                     /locus_tag="PC414_002175"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HDF2464570.1"
                     /translation="MARELELKYGCNPNQKPSKIYMKNGELPIEVLNGKPGYINFLDA
                     FNSWQLVKELKEATGLPAATSFKHVSPAGAAVGVPLSDTLKQIYFVDDLELSPLACAY
                     AMARGADRMSSYGDFIALSDVCDKETATIIAREVSDGIIAPGYTDEALEILKGKRKGN
                     YNIVKIDENYTPEPIETKDVYGITFEQGRNEILINEDLLKDIPTDNKIFTDSAKRDLI
                     IALITLKYTQSNSVCYAKDGQVIGVGAGQQSRIHCTRLAGNKADTWYLRQHPKVLNLK
                     FKKDIGRPDRDNTIDVYLSDDYMDVLADGIWQNFFEEKPEPLTGEGKRAWLKTLTGVA
                     LGSDAFFPFGDNIERAKRSGVSFIAQPGGSIRDDNVILTCNKYNIVMAFTKNRLFHH"
     gene            complement(26200..27084)
                     /locus_tag="PC414_002176"
     CDS             complement(26200..27084)
                     /locus_tag="PC414_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HDF2464571.1"
                     /translation="MKFVTFCSSNEEKIGVFNSETNSIYEINSLGLSKLYTDMNDFIE
                     NVSTGDLEKIKNNSFENAKCYKLEEVKLCSPIVRPKKDIICLGLNYKDHVNEIPDGVI
                     KNVVMPDYPIYFSKRADKIIGVDDKISLHGDLVEKLDYESELAVIIGKEGINISKEDA
                     YEYIFGYTIVNDISERALQDKHVQWFRGKSLDTHTSMGPCIVHKEEFEHPLKLDISSV
                     VNGEVRQDSNTEYFIFDIPTIINDLSRGMTLKPGDIISTGTPAGVAMGMNPQVYLKHG
                     DIVECKVEGIGVLKNIVD"
BASE COUNT         8653 a         4857 c         2788 g        10850 t
ORIGIN      
        1 ttatataata agtattttta ggattctgtt acataatatt atttaaatat tcttctatat
       61 ttctcttctt cttcttcagt aaacacatta aatacaactt ttaaattttt ttcaggattt
      121 tcttttagcc atagtctaac tctacttaca gcaagattag ctgcctcttt ttttggataa
      181 ccaaatacac ctgtagaaat tccacaaaaa acaatatttt ttatttcatc aatttctttt
      241 attgtattta aacatgattt ataacaatga agcagttgct tttcttgctc tttacttggc
      301 tgaccaccag atacaatagg tccaacagta tgtactacaa attttgcagg taaacaataa
      361 cctctagtta tcttagcatc tccagtatat tctaaatgcc cttgtttttt tattattttg
      421 tcacaatctt ctctaagtct aggacctgcg catgaatgta tctcattatc cacgcataga
      481 tgtaatggtt gtaaacaccc taataacttg ttatttgcag catttactat tgcatctgct
      541 cttaaattcg ttatattacc tctccatatg gcaatccctt cctctatttc ttctatatca
      601 tttacatcta ctaactctct ttcttcacat tcaatcaaca ataaattatg taacatatta
      661 agtatctctg gagcaatttc ttttggtgga tatacattca aaagtcctct aagtatatct
      721 ctaggcttaa tttcatcatt tgataaattg tatattttag aaggaacttc ttttgaaaaa
      781 tatgcaatta aagtgtttat attttttctt ctttgttcgt cagttagagg cttgatagtt
      841 ttatcaaaat cctcaaataa atttacatca tcagcataat ctctccactt catagccatc
      901 tctccattct tgtattttag acttaaaaat aattatgtat ttatgtacat atttatttat
      961 ttctataaac acctacataa atcctgttaa aagtgtaaat tttttattat gcaacttctc
     1021 tggttatttc tattgattga tagtaaatta ttgtacctaa atttaatcca actaacaagt
     1081 aactaaacct actttcctaa ctgcaaaata cactatgttt gccacttgta tctcatttgt
     1141 aaataaataa agtccctaca aatacattaa ctaggctatg tactaaactg taggaacttt
     1201 aaaaatatat tattcttaat taagttttgg ccaataattt ttatttgctt ctattaaatc
     1261 atctagtaat tcttttgcaa ctgttgcact aggaactgtc ttagatagca taagtgcttg
     1321 ccacaattta ttataagact tctcaatcca agcatcaact actaattttt caactgctaa
     1381 ttgttgttct attaaacctt tttggaatgt tggtattttc cctattgata atggttcata
     1441 cccgtctttt ccaactatac atggaatttc aaccatagca tcatcttgta tattttctat
     1501 agctccatta ttttgaacta tcaataacat acgctcctgt gtattaaaag ctagagctgt
     1561 agctaaatcc actataaact ctgcatgtat tcctatctta agcccactgc ccttagaatg
     1621 tccttgcttt tctatcaatg cacatgaacc aaaaacttct ttttctcttc catcaataac
     1681 ttcattggca cgagtataat ttacatcaga atgatttaca acataatctg gaaatagata
     1741 gtactttaag taactacttg gtattgtagt taaatctgtt tttactattt ctttagtaaa
     1801 taaattagtt tcaaaccaac tatctttctt gtcatttttc attccttctt caccatttat
     1861 aaaaccatat tcttttatat gttctactaa ttttggtaaa agttcatttc cttttttatc
     1921 tttaattgaa gtataccaac caaagtggtt taatccaaag tatcttatat ccatatgttt
     1981 acgagatttg agtcctgcaa tccttgctat attatgttcc atacctattg gcatatcaca
     2041 aatatttatt accctagaat taggtctcaa ccttctaaca gcttccgcta ttattgatgc
     2101 tggattagaa taatttaaca tccaacaatt tggagaatac ttttccatat aatctatgtt
     2161 ttcaagaaca ccttctatag acctcattcc ataagctatt ccacctggtc cacaagtttc
     2221 ttgaccaact accccatgtt ttagaggtat cttttcatct agttctctca tttcaagttt
     2281 tccaactctt atatgagcta aacaaaaatc tacatctgta taagcctctt tagggcatgt
     2341 tgttgctaaa tactctatat tagggtcctt ttcctttatt agtatttcac aagctttagc
     2401 taccttattt tgtctttctt catcattatc atataatttg attgctctta atggtaacct
     2461 atctaagtta tctagcaaca ttaatatgat ttctggggta tatgtacttc ctcctccagc
     2521 tattactact gaaaattttt ccataataat atctcctttt ctatttaaaa atttactgta
     2581 tttataaata aatttataaa tactagtact ttaaagtaaa agggagttca tttattaaat
     2641 ctattgattt aataaactat agccaaactt caagcactat ttacaagaaa tttaattctt
     2701 attaattctt gggtccatca tctcaaatat atctacaagt tcagatacta aagaatgagc
     2761 aaataatgca tttgaataat ggtcttcaga gtgcacaact aaaaaatcag tcccactatc
     2821 ttcactattc atagcatttg atagtaattc taaatgatgc tttgatgctg tttgtatttt
     2881 ctctgaggct gaatttaaaa ggtgttttgc tttattgtag tcaccttttt ttatttcctt
     2941 aagagacatc attacatcac ttttagcatc tcctgcttct gatattatac taaatacttt
     3001 ttcttccatt tcaggtacca tatccttctc cttctaatga cttgttaaaa atcaaagcta
     3061 tcatcatcta atgttgtagc taattctgca tttttattta attcttcctt ctccattatc
     3121 ttaaagaatg gataatatat caaggtcata agtattatac ttgcaaatac taatacgatt
     3181 gccttccaat ctaatgtcat caaaaatgct gcaacccctg gaggtaaaaa gcctggtggt
     3241 tctgcaatag gtaatcctac taatcctact tttaatgcca taaaattaag tgctccaacc
     3301 aatacaggtc caaatacaaa tggtacaaac atatatgggt tcataactat tggtgttcca
     3361 aatattaccg gctcatttat tccaaatagt gatggtaaaa tcacaacttt tcccatacta
     3421 ttaaaactct tacacttact tcttgacata aggtacacta atcccaaagt tatccctgaa
     3481 ccagttactt gcaagaaacc atagtaaaaa cctattgtaa acacatattt tgggtctcct
     3541 cctgctgcta ttaacgttgc attttctgcc atatatgtag ctgctattgg catccatata
     3601 cttgaagtta ttgctgctcc atgaagccca aagaaccata ataattgagt tataaatagt
     3661 attatagtta ctgcaaaaac attatctaca ctgcttatag ctggtgctaa gaaattcatt
     3721 ataatagctg gtaatggttg acctgagaca tttttagtta ttgctgctat tgctgtaaat
     3781 aacattatac atactatcat tggagttaag tttctaaaac ttttatttac tacactaggt
     3841 acttcttttg gaaatctaat tacgagattt ttatcattca taaacttagt aatatcaact
     3901 actaaaagtg ctactaatat acttacaaat aaaccttgtg catctaaata acttgtatct
     3961 aatacaccat ctactacttg tccacataca acaagttgtg caacaactgc tgtcatacct
     4021 atttctacat gattaagatt atatttttta gctagtgcct gagctataaa aaatgctgaa
     4081 tatatagata ataaactcat agtaaattta ttagggataa gtaatgtatc catattttca
     4141 gataataatg tttttgcact tccttcaggt aacatattgt ttaatgccat aaataacata
     4201 cttaaagaac caacaattat tattggggta atgcttatca taccttcttt tatagcttga
     4261 agatgccttt gtttttcaat cttttgtgct attggaagaa gaaatcttct taaattattt
     4321 tgaagtgctt ccatttttat tccccccaat tatctatttt tatataattc gattgccgac
     4381 ttcataacag aaggagcatc catacgacca taatgcttag tatctattaa accaactgct
     4441 attttgtaat tcttacaagc ttcaactaca tcatcttcct taaacctaat ttgtggtgct
     4501 acaagtacta catcggtatc attagcttca atgtatgatt ttaaattttc aaatgatgtt
     4561 gcctctatat ttatagtttt agtttttaac ttttcatgct cactacaaaa acttttcatc
     4621 ttttccacaa gtacacctgt tgaagctcca tttgcacata ctaataaaat attcataatt
     4681 accctcctac aatatctaac ctcttttatt ttctaatttt ttatcagcca attcttactc
     4741 taattcctat cagtttttac tttaattctt attattttat tttaattctt attaatattt
     4801 actctaattc ttagtactgc caatgattaa acttgaaagc cgtatctata acttcttctt
     4861 gcttatattt ttctaatatt tttaagtatc tttctttata atcattatct acttcttttt
     4921 ctggtattac tatactagct tcatttaaaa agtggaatga atcccttccc atttctaatc
     4981 ctctatatgt atgcttgtca agtgccacgt ccataacttc tggaacttta cccattgcaa
     5041 atccttttat aattatattt tttaataaat cacttgatct atctttctca catgcacaaa
     5101 gatatcttat tgcatgtatg aaaaataaag gtctgtctcc atctgcatat gaatattcct
     5161 ttcttacttg atttaaatta tttatgatta ttacagctga tgggtccccc attcctatat
     5221 cttcaactga tattgtaagt atccttctcc atagcttatc ttctaattct ggtgatgata
     5281 tatatagttc ataagcaaat tcacaagctt cttccaccat atttcttctt atagcttttt
     5341 gaaatgacga tattatttca tctgcactaa atccgtttct tgtagtcatt cttgcccatg
     5401 ggtcatttgc ctttttatct cttaatattt tatcttgcat attatttact cctttttatt
     5461 taaattttaa ttatttatat tactcattta ataatataag caagtattat gccagttttt
     5521 ttagacactt taaaacactg ttatttcaat catttttatc atcaaaactt agatactttg
     5581 tctaagtata tttatatgtg tctaatatta atattatttt agactaaaaa aaattaccaa
     5641 ataagttttt atactcattg gtaatctttt tcgattattt ctgcatattc taatattaat
     5701 aatatttgat agcactcttt ttcattaatt tctatattat attttttatc taattgagat
     5761 actatatctt ttattttgct taggctcttg tgataatcaa gtgttttttc tttatcaagc
     5821 ttttcaattt ctctagtatt tccaatcaat ccatctacca tgaaagctaa atgcatcata
     5881 agtccaatta ctttatcgta tttcttttct atattaaata ctaaaaacaa ttcatcagac
     5941 acatatttta tatctttaaa tatcctatta taattagagc tttttaagga tgtctttaaa
     6001 ccttcaacta catcatcata acctatactt atctttataa tattttttaa tttattgata
     6061 ttttcatcct tatctatatc ttttttgcta atatagtcaa ttcctctaat attaattctt
     6121 tgttcattga ttactgcaac aatattacta ctttctttaa gtttcgtaat atttaaactt
     6181 aaatcttttt ctgttttata tgggacagat ataatatcta ttccatctaa gtacctactc
     6241 atcttttctt ctaatatttt tcttgtttta ttatctacac cttctgaaac atgtgatgct
     6301 ataataattg ttttagacag attttcttta ttttgaattt cattagttcc aaaatagtac
     6361 tttctcgtgt tagttataga gttgtatacg tcatttaaag gtaatcccat attggccttc
     6421 tgaactgctt ctaacacagt taatgttgtc accataggaa ttactcttac tccacacttt
     6481 ttttctttct ttataatttc atcaaaagag tttaatgaac ccatatctgt aaatagtaat
     6541 acatctttat tgtctatatt attaattaaa tttttaaaat ctacaataac ttcatcatag
     6601 cttttatcta acttcatatc gaaagaaata gcactgcact ttccaataat atcaccaact
     6661 gcttttacca tactacttgc tgtagtttct ccatgcatag caactataat attaatgtta
     6721 ttcactattt tattttgttc atcttgttta gcttgtacta tgaataatgt aatatatgct
     6781 acagtttcta tagataaaaa taaatcaaat tcattttcaa ttattccaac tatttttagt
     6841 gctgttttaa attcacttct gttatttctt ctaatttgat tcaaatctat gttgtttgta
     6901 gagctacttt tatccaatac gctttttata taaattgcca ttgacttaaa tattgattca
     6961 cttatgtcta tttcttcatc ttctttaata atttgtctta ttgattcaca catgctttta
     7021 aacttaacat tattaaatag agaagcatct gctatattca tttctacaga tttattaaga
     7081 ttagtattta ctgcattaat aatatttata tcattgtagt taataacttc ttcttctcct
     7141 aatgacgtaa atacaaaata ttctattcca tgtgttataa cttcatctgc tttaatagac
     7201 ttctctctat aatcatcact cacttcattt atagatttat caaaatattt tttctctata
     7261 taaactggtt ctttattatc tcttttatat cttaggtatc catttgcaac agctaatttt
     7321 atatcatttt gaagttgacc tacattattt ttacaatcat aaagtaatag tgatttaatt
     7381 atttctttat gtacttttat tgaaatagaa atactttttg attcttcctt gaaaaaattt
     7441 ttgactaatt ccattctttc aatcattgtc ctatctctca gtgatggtat ttctattttc
     7501 attggtattc ttctattgaa tgtatctaat agtgttgatg tcggattttc tgtagtagca
     7561 catattatta ataccttaga ctctctttct ttcgttgcat ctcctagtct tttaaatgtt
     7621 cccttatcta taaaagtaaa caacatttct tgaccttctg ctggtagtct atgaatttca
     7681 tctaaaaata gtatccctcc atttgcaatt tccaaaagac cttttctgga agattctgca
     7741 cctgtataag ctccttgttc taccccaaaa atatgtccca taagtaattg tgcattatta
     7801 gcataatcag cacaattaaa tgttacaaaa ggagctttat tttttctaat tccactatct
     7861 atagaataat cgtacatata tctagcaatc atactctttc ctactcctgt ttctccatag
     7921 ataatagtat gaagtccatt tgtaggatag agcattgcga ccttgcattt ttctattata
     7981 ggtcttaaac tatgttcttt acctattagt gtttcaaaac tatctttttt tattttgttt
     8041 tctacttttg ttcctttttt tatgccaagc aactcacttt gtgtatcaag caattcattc
     8101 ttgttaaggt taagatttgc agggatataa tacacaggtc ttccttttat cttcgtaacc
     8161 ataccctcat tgaataattg gtttaaatat gtacttatat tgtttcttgc aattcctgtc
     8221 ttttctgata attggttagc tgtaatccct ttttcatttt ccctatatga tgattctaaa
     8281 actttcatta ctttatcttt tttatatatc attacaagct cccctacatt atagtatttt
     8341 attaactaaa acccctatac acactttatt ttttaataaa ctcttattct ttaactaagg
     8401 ttataattta tcaaactttc cttactttaa attatagaaa attattgtag aaaaaataag
     8461 attattgctc attttttcta cattactatt tttaatttta tagtactttc ttaacaacat
     8521 actttacttg tttttaattt tattatattt gaatttatta tatttgaaaa gtagaaataa
     8581 aaaaccctat tttggagtag ttttaaaatt tctccaaaaa tagggtttta gtttgtattt
     8641 caaataaata cctgttcaag tatttttata ctaaacaact tttaaatttt gttgatattt
     8701 atttatttct gatttagaag aatatacaaa atgtccatct gaaacatcaa tccaacttaa
     8761 tgacttttca tttgaacaat catatccatt agaattatac tctattctat ttcttgactt
     8821 agcatatcta gggtcaggaa caggaattgc agaaagtagt gattttgtat atggatgaat
     8881 aggattatta tacaattcat ttgaatctgc caattctact aattttccat tatacataac
     8941 tcctatacgg tcactaatat gtctcaccat agataaatca tgagctataa ataaacaagt
     9001 taaattcttt tcttgttgca attttttaaa taaatttact atttgtgctt gtatagacac
     9061 atccaaagaa gctataggct catcagctat tataaattct gggtctactg aaagagctcg
     9121 tgctatgcca atacgctgtc tttgtccacc tgaaaaatca gatggatact tatttgcata
     9181 acttctatca agtcctacta gctttagcat ctcatatact ttattcattc tatctgtttt
     9241 atttttacag ataccttgaa tttttagtgg ctctgaaatt atatctgcaa ttgtcatacg
     9301 tggatttaat gatgaagtag aatcttgaaa aataatctgc atacttttgt ttacatcttt
     9361 ttttataaat ttgtacgttt ttttatctga tatgcaattt cctttgtaaa ttacttctcc
     9421 accagtagat tcatgtagtc ttattatagt tttaccagtt gtagattttc ctgaacccga
     9481 ctctcctacc aatccaaata tctcaccctt atatatatca aaacttatat catcaactgc
     9541 ttttacagta gtacttttgt ccaaatgaaa atattgcttt aagtttttta tttccagtat
     9601 tttttctcta ttcattagaa atcacccttc cacaatttac tctaactggt acatctactt
     9661 caggtgcatc tgggtgcaat aaccaagttg ctgcactatg tgtatcatta attttaaaca
     9721 ttggtggctc tttttcataa tctatcttaa gtgcattttt attccttatt gcaaaagcat
     9781 ctccttttgg tgggttcaat aaatttggag gcattccagg tatattatat aaatagtcat
     9841 cctgcgaatc taaagtaggt aatgaaccca acaaccccca agtatatgga tgtctagggt
     9901 cataaaatat atcctctaca gttcctattt ctacaatctt tcctgcatac ataactgcta
     9961 ttctatcagc tatagttgca actactccta aatcatgagt tataaatatt attgacaaac
    10021 ctattttatg ttgtaaatcc tttattaaat caattatttg agcttgtata gttacatcta
    10081 aagcagttgt aggttcatca gcaatcaata catctggatt acaagcaagt gctatagcta
    10141 ttactatacg ttgtctcatt ccacctgaaa aatgatgtgg aaattgttta aatcttttct
    10201 caggattatc aattccaacc aattcaataa gctctatggc tctctttttt gcctcacttt
    10261 tacttatacc ttgatgaatg attacagctt ctgcaatttg ctttcctatt gatatagttg
    10321 gattgagaga tgtcataggg tcttgaaata tcattgatat attctttcct cttatatctt
    10381 ccatctcttt ttcagacttc ttaaccaaat cacttgattt aagtaaaacc tctccatttt
    10441 taatatatcc attgtatggg agtatcctca tcaaactctt acacagtaca gatttcccac
    10501 aaccagattc tccaactata gccaatgtct cacctttttt tagattaaaa gatacatttc
    10561 ttacagcctg aacttctcct tcttctactt taaaacttac agatagatta ttaacctcca
    10621 ataaatgttc caaaaaccat caacccctat gctattcaaa ccttagggat atatttttat
    10681 ccctaaggtt tctaattaat ttcagtatta ttcaattgtc cagtcagcta tgttccagaa
    10741 tatacctaca ccatgatgac ctaaaactgt atcaggtgtt aatcctttta tgtttggttt
    10801 tccaacatat attgcatcta tataagcaat aaatgtataa ggcatatcct tagtcatttc
    10861 tacttggaat tgtttatata actttaattt ttcatcttta tcttctgttt ctcttgcttt
    10921 ttgaagtatt ttatctatag taggattaga gtaagcactg taatttgcac ctttatctgt
    10981 tccaaatact ttatatgtat ggtcatctgg gtcaaatgga cttccccaac ctattaaatg
    11041 agcatcttga ttagcccaat ctgtttcagt tacaacaaca gctttagcat caacaccaat
    11101 ttcttttaac tgttgtgcac atattttagc catatcaact ctaacttggt cactctcacc
    11161 tgctgttatc tcgaaagcta acttagtacc ttctttttca tagattccat cagaacctaa
    11221 tttccaacct agcttttcaa tttcttgttt agctttttct ggattatatt caaacttttc
    11281 tatatcagga ttattatatg gtcccatttg taaaggtgaa tatgctggta caccatgtcc
    11341 taataataca ctgtcaacta ttgcttttct atctattgca tagcttaaag catttggtaa
    11401 tcctttagct tttttatctt taaagaattt agaattaaaa ttgtataata tacctctata
    11461 gtcggctgtt ttcattatat ttactttaaa attcttttcg tctttttcaa agttagacat
    11521 atctttagga gttatttgag ctaggtctaa ttctcctgat tttaattgca ttgccttagc
    11581 cttatcatct ggaacaatct taaagactac tttatctaat cctggttctt ttacaaaata
    11641 atcactattc ttaacaagtg ttatactttg acctttgtcc catttttcaa gtttaaatgg
    11701 tccagtacct attggttttt gattaaattc atcagtagct atatccttac cttccaaagc
    11761 atgttttggt aatactccta ctgttaaata atcaagcatt gctgtatttg gagcttttaa
    11821 agtaattttt atagtattat cattaaccac atcaattttt gttatatctt cataatttga
    11881 tgctatctca gaagcattat ctggattcat tatagtttct attgtaaact taacatcatt
    11941 tgctgtaaat ttttctccat catgccactt aacatcatct cttaaattaa aagtatatgt
    12001 atttgttgcc tcatcaaatt tccagtcctt tgctagacaa ggaaccacct tattattttc
    12061 atcatgagct gttaatccat taaatattaa cgaatttatt tctccatgtt catatagtgc
    12121 aggatttata cttgtatagt cattacttcc ataaacaagc acctttccat ctttaggcgt
    12181 atctgccttc ttatctttgt ctcctccact agaacaacct gccattagac taagtatcat
    12241 tactaaactt aaaactttta acttctttaa tttcatgatg ctcccccttt tgctttttat
    12301 aaaatatttt tcaaagcttt gaccccattt atttaaatag atatttcaaa tacctcagta
    12361 tttgcttatt taatactcaa atgctgattt ataaattact agatttttta ttattattct
    12421 ttcttatata attacctata tttgttatac acaccaatgt ggtaaccaaa aatatacccg
    12481 gaatcagtat aatccaccat ctatttgaaa gtaatgcttc ctctgacaaa gataacatac
    12541 ttccccaaga aacaatttct attggtaaac caattcctaa aaagctaagt gtcgattcag
    12601 taccaatagc tgcgcctatg tttgttacaa ccatgaacat tattgatgcc acaaaatttg
    12661 gaagtaaatg ttgctttaat acatagaaga atcctcctcc catgctctta gctgctagta
    12721 tgtattcgct attttttatt tgtctaactt ctgttctcac tatcttagat atattcatcc
    12781 aacttgttat ccctattact atggacatag atactggatt tgaatttcca agtatagctt
    12841 gtacaaatat tataattaat attgaaggta tactaagtat gatttctgta aacctcatca
    12901 ttgcatcatc tacaagttca gatgctgaac cacttatact accataaaca attcctataa
    12961 cagttgatat tacagtggaa aatagtccta taaataaaga tatttttcct ccataccata
    13021 tcatagaata tatatctcta cccattgagt ctgttccaaa taaaaaagtt ttatttggag
    13081 ctaaattaga acttgctaaa tccatgtatg ttggttcatg tgtcattata aaagatgaga
    13141 atacacttcc aatgactatg attgataaaa taataattga tatatatggt aattctttaa
    13201 tttttttaga taaggttttc tttgtttcta tatgttcatc ttctgctact aaaatataat
    13261 tttctcctac aatttcaaaa tcactatatt gtggcttcat tagtatcacc tctatcatat
    13321 ttcattctag ggtctatttt attgtttata atttgagcta acatattccc aaatactact
    13381 aaagcccctg ttattaagca taaaactaat aacatattat aatcatgata ctttgcactt
    13441 tcaaaacaaa gtgttcctaa cccaggataa gaaaaaactt tttcaactac ataagttcct
    13501 cctagtatat gaggtatcga aatggccatt atactaatat atgatggcat tatatttctc
    13561 aggcaatgct taaaaacaat agttctatta tttaaacctt ttgctttaca taaaagcaca
    13621 taatcttctc ttatttcctc tagtaattta tttcttatca tatatgtgta ataccataga
    13681 tggcttaata taagtactgt aagaggtaag attagatgtg atatcctact taatatactg
    13741 gactcttctc ccattgaata agcaccacta cttggaagta tacttaagtt tatactaaat
    13801 ataagtatga gtactaaggc aacccaaaat gatggaatac agttggttat tgttcctaat
    13861 ttgcatatga ttctatcaat gagcttatct tcatgtaatg tacaaaatat tcctaacaat
    13921 aaggctagca caaaggtaag tatatatcct gaaccaccta atattatagt gtttatatag
    13981 acatcattta taacagaagt aacattttgt ttatatttaa atgaaattcc aaattcccct
    14041 tttgatgcat ttgaaatcca ttttatatat tgactgtata ttggttcatc caaaccaagt
    14101 tttttcatag cattttcttt ttcttgtgta ctcattcttt ctactccatc cccatagtaa
    14161 gagattaatg ggtctcctgg agcaagtctt gccatgtaaa aaacaataaa agataataaa
    14221 aacattacta aaataaactg caacaacttt ttcagtatat cctttaacaa accccaaccc
    14281 ccctttgttg caataaaaaa aaccacataa aagcaaacgt aaagtaacgt taaaaccttc
    14341 gtggttccat tatcattaaa caaaattaaa ttttctatat ttatctatga gttaatttta
    14401 gcatcattta ttaactgtgt aaacaaactt tttaaaaatt gttaaaattt catcaaatta
    14461 ttttatttat acctaaaata aaatttttat attctattct tcattatttt ctataaaatt
    14521 ataaaatttt aggttgacta tataagaaaa gtatattata ttagtttgta tgcaaaccat
    14581 ttaagaggtg attttattga aaagtaatta tgactgtttt agaatagcca tgctcttaaa
    14641 agaactttat tctaaaacta tgtacacagt agaagaaaat tttaaagaaa atggattaac
    14701 acaccaacag attatagtta taaaactgat agctcataat caagaactta caatatcaca
    14761 actatgtgat gaaatgtctt tagctaaagg tacggtttca ggcataatca gcagattaga
    14821 acagattgga tatatagaaa aattcaagaa atctaatgat aaaagaaata catacgttaa
    14881 gtttacaaca acaggatttg aatttgccac aaattttaaa attaaaatgc aagaaagttt
    14941 tgatgatatt tttaaaaatt gtgatgaaaa cgaattgagt gacttagtaa aaaatcttag
    15001 aaatatattg gcaaaagtaa aggagagata attatgaatt ataaattaat acttgcaata
    15061 gtatttataa catcagcttt aattttctac actataggtg tatttggaga aagaaaggca
    15121 aaaattttaa aaaagaaaca tgtaattata ttttggcttg gttttatatt tgatacttta
    15181 ggaacattta caatgagtaa catagccaac agccatactt ttgaagtaaa atctgcttta
    15241 tcacaaaacc ttcatagtat tacaggtctt cttgcaattg tgcttatgct ttttcatgca
    15301 agttgggcta cttttgtcct atacaaagat gatgaggaaa agaaaaaatt ctttcataag
    15361 tttagtatag ttgtatggac tatatggtta gttccatatt ttataggtat gtttattggt
    15421 atggctggat agtaaaataa aaagtgtgta agaaatactg taggtaaatt tgtataagac
    15481 aaatttatct acatccttct ttcacacttt tacatattta tttataatac tccattattt
    15541 ctttcatgtt tcttaatacc tatatagaat atttaattgt ttctatagta ttttattatt
    15601 ttctcacact ttttcatata tatattgttt tactatttct tcatatgtat atatgaaata
    15661 ttccattatt tctataaatg ttttattatc tttctcatac tttttcatat atatattgct
    15721 tcactatttt ttcttatttc ttcatataca tatgaaatat ttcattattt ctataatgtt
    15781 ttattatctt tctcatactt tttcatatat attgcttcac tatttttttt acttctttat
    15841 taaatactta tgtataacat acatttaata aacgctaact attaaacacc taatacttga
    15901 gttactcctg gaacttcact caccaaaaca ttttcaataa tagctttgat tgtcatagtc
    15961 actcctggac acccactaca tgccccttgt agtctaacta aaaccacacc attttcattt
    16021 acatctataa gctctacatc tccaccatct ctttgtaata ctggttttat cttttcctca
    16081 agcacttttt caactttttc tctcatagct attcccccca gaaatatttt attagcaaaa
    16141 ttataccaat ggtttaaatt tttgtccatc taatgattta tcaaattcaa tttttgcttc
    16201 atcaataatt ttagtatcca taaatacatc ataagcacta caagccagta ctttagctga
    16261 atttatcata gcagaaaatc ctatatttga acctgctgat gaacatgact gccatgtatg
    16321 acttgcaatt ccaacaggcc aagctgccat tgcaaattga gccactggta ttacataact
    16381 tacatctcca acatcagtag aaccagctag tcctttatct ttgtcaccgt caactatttc
    16441 cttgtgtata tagctttcta caatagattt atcattttgt aatgatgttg ctacacctaa
    16501 tctttcttct ctagtaagag tatcacacaa cttttttgca aattcttctt cttcaggagt
    16561 atttttaggg caaccaacaa aaaccatatt gtcataaact aaatcagtca aacattggtt
    16621 tggtatatag tcatatattc catctgtaac cttatactcc atttcagttt ctgtcatcat
    16681 tgctgctcct tgagctactt ttattaatct atcaagaacg tgttctgcat cctttgcttt
    16741 tttacctcta ataaagtacc aactttcagc aaatccagga actacattag gtcttccacc
    16801 accattagtt ataacatagt gcattcttat agagtcaata acatgttctc tcaaataatt
    16861 agctccaaca ttcataattt caacagcatc aagtgcgctt ctaccattat gaggagcttg
    16921 agctgcatgg gctgttatcc ctttaaattt aaattttaca gataagtttg ccagacttcc
    16981 acctctccaa ggtgcattta tatcaaaagg atgccatgta aatgcacaat ctacatcatc
    17041 aaaacagcca tttatacaca taactgtctt tcctccacct tcttcttcag caggacaacc
    17101 aaaatatttt attgttccat taaatttatt ttcttctatt aattttttta tagaaacaac
    17161 tgctcctaca cctgctacac ccaacaggtt gtgcccacat ccatgtccag cttttccttc
    17221 taccaatggc tttctttctg cacttacaca ttgtgatagc tctggtaaag catcatattc
    17281 tccaagtatg ccaatcacag gtttaccctc tccaaaactt gctataaatc cagtaggtaa
    17341 attttctact tcttcaattt taaatccttt tgactttagg tattcttttt gaatgctact
    17401 tgcatacttt tcttcaaagg ctaattctgg attttcccat attttcttag ataaatcaat
    17461 taattcttct cttatttctt caacattatt tatgatatac tctttcataa ttttcctcca
    17521 aatatatgta tttttgatat gctttacaga ttatattata tactaacatt ctatttattc
    17581 acaatttttt gttaattaat tttattcata tgctcttcta tggagtatat cttatcttca
    17641 ctttgcctct tcataaattt aatgtactct atcttccata aagtagtttc agttttctca
    17701 tttaacataa attctaatat attaatttct ctcattctaa tatcttcata aaatactaca
    17761 ctacccaata ttgccaattc taattcactt aatgatgcaa acaacattaa atactcttct
    17821 ccaaataaac attcagattc aagaatatcg ataaagtctt ttttagcatt gatattttcc
    17881 tttattattc ttattttttc aattatagat gtactatcta atatctcatt gaatatctct
    17941 ctaaacgatt catcatccaa tttactttca tcatcaacaa tataatcttc taagtgttcc
    18001 tttttatttt tactcacagt taccatactt ttaaaagtgt tactttttat tgagcttagc
    18061 acagattcaa taaatatctt ttcatatttt ttaatatatc ttattacatc ttcatcttct
    18121 atgcttaatc tttttattaa tacttcaatt gcttccataa gtagtaaagt tattttttta
    18181 tttattctaa tttctctgtt aatttcttca aaatcattat aatccaaatt actatctact
    18241 tcagtgttaa aactatcatc tacttcaatc aattcaatta tattactttc ttcagagtct
    18301 ataactttat cttcagtaat attattgtac atatttttaa attgtttttc taaaaattca
    18361 tactcatatt ttgaaatttc taaattagtc acttcatttc ctaaaatagt agaaaaaata
    18421 caattattca taataagttc aaataaattt acaagtataa catcacaatc tattttataa
    18481 gtcatcccat agtcataaaa taatttttct ctatcttttt tcttaaatag gttacaaaat
    18541 ctattttcta tatctatact ctcaatataa ttcttcacat aataaatacc cttaacatcc
    18601 atatcatcta gtgccaatgg ataatctata cttactggaa cgtcctgagc ttcaaatttt
    18661 acaatataac ttttaaagaa acttgataac tccatcaagg tatcattata tgctattgaa
    18721 ttagtatcca attttctact catcattagt ttataaaaat cttttagcct aactaactct
    18781 tcctctaata tttcaatgcc tccaaggtac acttttttaa cattcttagc agtcaactcc
    18841 acaactcttt tttctatatc tccaagctta tttatatatg catcaatagc ataccatatt
    18901 gaaatcaaca gtttttcggc aacttctgtt tttactgatg tgctttgacc atttgtatat
    18961 tttttaatca agtccataag tattagagat acttcttgtt gtatattata tacttctctt
    19021 tcatctatca ctttacaatt tagacattcc ttaagtagag atatggaata ttggttcttt
    19081 attaaattat tttcgtttat attaatatta cttaaattat ttaacataca aaatctctcc
    19141 tatcatatat taaacttctc caaatttaaa tttctttgca aattcgtctg cttctcctct
    19201 tacattgtcc atattgcctg aaaatttttc ataaaattca cccagtattt ctataatctc
    19261 atcatcacat atcttgtcat cagtttcatt ttttaaataa taaaaaattt cttgcatatc
    19321 attaatagcc tctaaataat tgtccacatt tgtatattgt gatgtatata tattttctat
    19381 taactgtttt gtaactttta tatctaattc tattcttcca taccccttta aagtattatc
    19441 tctcgaattt attatatctt ttacatcaga ttcagaaaga gctagaccat aatctccaga
    19501 ttctatatta gtttcaagca gttcatttct ttctatttcc tttaaattct gatttataga
    19561 actcaaaata ataggtgata tcatttttaa attctccttt tttatttttt ccattgactt
    19621 tttttgtttt ttatgctata cttaaattaa tgtatattat tttaccaacc acttataata
    19681 gtagtgttaa aagtggtttt tgtcaactca aaattactat ctcaattaaa cttttcaatt
    19741 ttctcaacaa aaaaatacca tcatttagat atataatttt ctataacaac ttttaattca
    19801 gtagttattt actaaaatct aaacaatgat atatttatta attttattta tcttttttgg
    19861 ctctacttct acttttcttt acatttgagc aatttctaca agaaggtgaa cagtattgag
    19921 atttagaatt tgtaggaata taaattttac cacagtactt gcatttattt aaagaagaag
    19981 gttcacttgt aattgcaaag gcatagatag tctcaatagc tgttttcaaa gaatcaaact
    20041 cccatgcaat aattggttta tctaattggc ttatagaaaa tcctatcttt tgaggattaa
    20101 accctccaga cataatagta acactttcag ttaatagatt agatgtattt ttatatacac
    20161 aaatttgatt aaaatgctta cacatatctt tagcaaaact tacaatccaa tctagttgtt
    20221 cagtgtagaa ctttgaaaat actaaatcta aaatcaatgg tctctttcca aaaaactttg
    20281 gagattcttc tctctttgat acatctatac cccttctata ctttttaaaa tacacatctt
    20341 catcacttgc aaatggtata aacatgttta catattcatc tgtactcaat atattctctt
    20401 ttgtaatata attcttttca atcatcagta ctctttcttc tccaactata tctctattat
    20461 atacacttga atatattaaa cccaataatc catacttttt tacaaattca agtacttctt
    20521 tctttagttt catttctttt gtctcttttg atatatcttg taatgcctct tgtcctatgc
    20581 gaattatatc tactattata tcatctgcta catcaaatgg gttatacatg gtaaacacag
    20641 catcttctgt tggaactata tataattcat cttcttcagt aattttatat tcataatcac
    20701 tatacctaat ccaatcactt gcaaaattcc ctattaattc tattttttta ttcataataa
    20761 tcattcacac ctttcaatgt aatatataac ttcatattac tattacactt ttagtttatt
    20821 ccatattttc tacactgtca actattcttt ttatattttt agcatattaa aatttataat
    20881 agcctttata atggattttt acagctaaat ttcttgttta ttgatgacta attcataatt
    20941 aaatttataa aaactattta catataaaat taatctcttt ataattgata tgtaatatat
    21001 ttttacactt acatattaca tatcaattat aaagagaggt atctaaaatc atggatagaa
    21061 aacgtgaatt aaaacaatta tacaaagaaa tgaaatttga cacaggtgta ttcataataa
    21121 aaaatgatat tactaaaaag atttaccttg gaaaaagtaa tgacataaaa agcaaattta
    21181 actctctcaa atttcagctt ggtgctggct catgtatgat aaaagaatta aataatgaat
    21241 ggaaaaaata tggtgaaaaa gcatttacat ttgaagtttt agaacttata aaacatgatg
    21301 ataacaaaac agagaaagac tatttagaag aactagatat tttagagatg gtttggttag
    21361 aaaaattaaa ggaagataat acttatgaaa tttattccat ataaaaaata atataatata
    21421 agaaaagtag atagtgtctc aaaatagaca tttagttcat aagatacttt tttatataaa
    21481 ataaagtatc ttttcataat tttaacaatg aaaaagaggc ttatctccat ttgatagcct
    21541 cttcccatat attttatacc attataaaat gaacaatcta ctaattttca atgtatatat
    21601 tcattttact aataacactc cattacttct ttacagcatc caaacattgc tctataatac
    21661 tcttatcact tatacctatt gctgttactt tactagctcc acttaatata ctcttatcac
    21721 ttttatttga aactaaaaca acaggagcat tttttgcaag tggtgatgca actaaagcat
    21781 aaactaaatc atctccactt gttatataga actcttttgc tccattatag aacttttcaa
    21841 taacattttt attagtttga tatctatcaa caccaccaat tctagttgct ctatatctgc
    21901 tcactatgta atcacttaat gtagactctc ctccaatagc atatactgga acaggagaaa
    21961 ctccaaacca tgggtctaag tctgtgtctt cttctgctga aggtatatct tttgtcaata
    22021 ttataggagc tccatctcta taagcaacag atgcaacact tactgcatca gcttcatcct
    22081 taaatccatt tactaaaaac atcttatcac tgtttttaat tttttctatt tcacctgcaa
    22141 ttttataact tgtatctact ctatctacac catcaattct tataacttgc attcttctca
    22201 taagtaacat tttttctaca tctttactta tagaattttc tccacctatt acatatatct
    22261 tagtaactct attcagtctt tgaagtgttg cttctggtat atcatcctta ttagttaata
    22321 aaatagctgc attatttact cctgcaagac cacttgcagc aatcccatct accattgtag
    22381 agtctgcatt gataagaact gctgttgtaa aatcttgctt atctgaaata agcccagctg
    22441 tttcatattt atctactcct tgaatatttt caatcttatc tagtgcgttt attgatgttg
    22501 gaaaactaac acttaacagt aatgctgaaa tacctaacgc caataccttt ttactaatcc
    22561 tcataaatta cctccctaaa acaaatatat ttagtattag tataatataa aatcatttaa
    22621 aaatagtgta aaaatagtta cactttttta taatttaaat aattttagac taaacacaat
    22681 tcatccatta aaataacttc atttattcta tatgctaagc catcccacgt tttttttgac
    22741 atatctacag atgttttctt atttgagtta ttcaataaag attgtatctt agaatctatt
    22801 gcattataca aattattttc aaaatcatat aactcttcat cttttggaat accttctttc
    22861 tccataaaag gtagagaaac atattctatc tttccagaat tattaatctc actaccaatc
    22921 cactctttaa caccaagaat atcagtagtg attacatctg tgccacaaga taaagcttct
    22981 aaaactacaa ctggtaatcc ctcataaaat gatggtaaca caaatatttg tgacctgttg
    23041 aatagctcag ctaaatctct ctgttctaac tttcctagga aattcatttt aaatggagac
    23101 ttacttgcca gtttaactat ctcattatat gattcgatat cgcttccagt acctgcaaaa
    23161 ttgacttcta ttaaatcttt actatatttt aatttagata tagcttttat aaatggaatc
    23221 agtccctttg atttacatat cttacctgca aatactattt ttattttatc atcctttatt
    23281 ttataatttt tattataaaa tatatcatca ttataaccac taccaattac aactacttta
    23341 ctttcactta ctccaaaagt ctttataata tcatatttct gattttcatg aagagcaaat
    23401 attaaatcta atttccttat atttgctatt atgtattctt tctctaaatc aatggtattt
    23461 aactgtctta aacaagttcc atgacaaata gacataacct ttatatcttt taccatttcc
    23521 ctaacaaatg cagttaagag gtacaaatga tgacatatta taagttctgg tttaaaatca
    23581 ttcatagcct tatctatatt tactttaaat tgatatttca atctatttat catgtctata
    23641 tttaaatctt tatatcttgt actttcatat ggcatagaat cactcatacc tacaacagga
    23701 aaatttaact ccccacaatt atactttact ggataaaaac ttacttcact tggaaaacaa
    23761 tttacatcat catttacatc tatacctgca attacagctt gcttatgacc gattttttcg
    23821 aaccctttta tcataccaga caaatatata ccactacctg tactatttgg tttttgtgtt
    23881 attatatgaa gaattttcac ttttcacttg cctcctttct aaaattaaat agttttatac
    23941 agtctaatca ttatatatat ctaaatataa acaagatgaa tcatctttta ctttaaatct
    24001 tggcacttta accgctttat agtcattatc ttcaaaatcc cttactttat tatgtatata
    24061 tcctatacca aatttttctg ctatacgtat caattcttct tcttttatta gattatatct
    24121 atcacatgca cttgtaaaac catcactcgt tagcatcaat tgaaaatctt tttttatttc
    24181 aatgtatcca tatatagaat tttccacagc ttcctcatca aaatccaata tccaatagcc
    24241 ctcttttgta tttttcttca atctattgct tactattgta tccataacac tagttctaat
    24301 ctcatcaaaa gccatctctt ccaagttaga aagtttactc atttcatcaa atacagtatt
    24361 atcaaattta caaatactcc tatcttttat aactttggaa tctccatttt taataaacaa
    24421 agcacaatct cctaataaaa aatactctac tttattttca tgaaatttta caacaactat
    24481 agaactagat ggtaaatcta atttttcaac cttaaaccca tttaatctta gttcatactc
    24541 tttttttact tctattattc cagtattaat aatactcttt aatgattctt ctttatttat
    24601 atttttatat aaatacctat tccaccactg aacataccac cttgcgtcac tttcagttga
    24661 cactaaattt tttccattaa gtcctgtagc accatctaaa acccatgccc caaatggatt
    24721 tataccgact acatcttcat taaatttagc tgttccttga ttacataata ggctatagtt
    24781 tcttatattt aaagccatat caacctccaa attgaatatc taaaaataaa gttctattta
    24841 aaattataca gcaaataaaa aaatctacaa caaaacttgt tgtagatttt tacttaatct
    24901 atattatctt tatactttca gtattttaat gatggaataa tctattttta gtaaatgcca
    24961 tgactatgtt atacttatta caagttaata taacattatc atcacgaata gaaccacctg
    25021 gttgtgctat aaaacttacc ccacttcttt ttgcacgttc tatattatct ccaaatggga
    25081 aaaatgcatc tgaaccaagt gctactcctg ttaaagtttt aagccatgct ctttttcctt
    25141 ctccagttag aggctctggt ttttcttcaa aaaagttttg ccatatgcca tcagcaagca
    25201 catccatata atcatcagaa agatatacat caatagtgtt atctctatct ggacgaccta
    25261 tatccttttt aaattttaag tttaatactt taggatgttg acgaagatac caagtatcag
    25321 ctttgttacc tgcaagtcta gtacaatgaa tacgtgattg ttgacctgca cctaccccta
    25381 ttacttgtcc atctttagca taacaaacag aatttgattg tgtatatttt aatgtaataa
    25441 gagctataat taaatcacgt tttgcactat ctgtaaaaat tttattatca gttggaatat
    25501 cttttaatag gtcttcattt attaatattt catttctacc ttgttcaaaa gtaattccat
    25561 aaacatcctt tgtttcaata ggttcaggag tataattttc atctattttt acaatattat
    25621 agttaccttt acgctttcct tttaatattt caagggcttc atctgtatat cctggagcta
    25681 ttataccatc agaaacctct cttgctataa tagttgcagt ctccttatca catacatctg
    25741 aaagtgctat aaaatcgcca taagaagaca ttctatctgc tcctcttgcc attgcataag
    25801 cacaagctaa tggagaaagc tctaaatcat caacaaaata tatttgcttt aaagtatctg
    25861 ataaaggtac ccctactgct gctcctgctg gacttacatg tttaaatgaa gttgctgctg
    25921 gaagacctgt agcttctttt aattccttca caagttgcca actattaaat gcatctaaaa
    25981 aatttatgta tccaggtttt ccatttaata cctctatagg aagttctcca ttcttcatat
    26041 aaatctttga tggtttttga tttggattac agccatactt taattctagt tctcttgcca
    26101 tatactcatc cctctctata ataaattaat cataagtttg attaatccta tattaattaa
    26161 attattcgta ctaataaaat tatatggcat atttatccat taatcaacta tattttttaa
    26221 aactcctatt ccttcgactt tacattcaac tatatcgcca tgctttaaat atacttgagg
    26281 attcatcccc atagcaaccc ctgctggagt tccagttgaa attatatctc ctggctttag
    26341 agtcattcct cttgataaat catttattat agtaggtata tcaaatataa agtactctgt
    26401 attagaatct tgtcttactt ctccatttac tacactactt atatctaatt ttaaaggatg
    26461 ttcaaattct tctttatgta ctatacaagg tcccatagat gtatgtgtgt ctaagctttt
    26521 tcctctaaac cattgaacat gtttatcttg aagtgctctt tcacttatat catttactat
    26581 tgtatatcca aatatatatt cataagcatc ttcttttgat atatttatac cttctttacc
    26641 tatgattact gcaagttctg attcatagtc caacttctct accaaatctc catgtaagct
    26701 tatcttatca tccactccaa ttattttgtc tgctctctta gaaaaataaa ttggataatc
    26761 tggcataact acgtttttta taactccatc tgggatttca ttgacatggt ctttatagtt
    26821 tagacctaaa catataatat cttttttagg tcttacaatt ggggaacata atttaacttc
    26881 ttctaactta taacactttg cgttttcaaa tgaattgttt tttatttttt ctaaatctcc
    26941 tgtagatacg ttttcaataa aatcattcat atcagtatat aatttactta atcctaaact
    27001 attaatttca tatatactat ttgtttctga attaaataca cctatttttt cttcattgct
    27061 actacaaaaa gtcacaaatt tcatttttat tcctccattc aataagttga ttaatattat
    27121 ttaattttta ttagtttata taaattgt
//
LOCUS       DAMJVK010000050        25700 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.051, whole genome shotgun sequence.
ACCESSION   DAMJVK010000050 DAMJVK010000000
VERSION     DAMJVK010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25700
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.051"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(1..2493)
                     /locus_tag="PC414_002177"
     CDS             complement(1..2493)
                     /locus_tag="PC414_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2464572.1"
                     /translation="MNHNTSIKNKDSNPKIFLVLTLYLLGIFMGAIDTGIVSPARTII
                     QNSLGVNEKTGIWMITIYTLTYASVIPISGKLADKIGRKYVYLVSIFLFGSGSLICGL
                     SSLFSNFYILLIGRVIQAIGGGGIMPIATAEFGTTFPENKRGMALGLVGATYGIANIL
                     GSSIGSTILSIFGTQNWKWLFFVNLPICLIILIGGVFCIKNNKSESKEKIDKLGTLML
                     ICIIVSLLYGLMNIDFFNFKNSIQDISVYPYLLTFIVLIPIFIFIENRAKDPILSFEY
                     FLNPRILIVLILSLIVGIGMMGMVFVPQYAENALKINAGSGGYFVAILGLFAGIAAPL
                     SGRLIDKFGAKKILLLGFSVSMIGSLYLVLIALKTNTVFSVCVSLIFMGLGMGFTMGT
                     PLNYMMLSNTRLEESNSALATLSLIRSIGTSISPAIMIGFIAHAGLSVQDNIMDLVGK
                     PSTPKIVQLEELENMIDDLKSDPEMAKNLKNVSIPNMNDTSNINMDMTGGKLPKSLLN
                     KVQSADTTNITNITKEISTTMFDEKVPSIITKIQKNVQKGIDGTQSGINGIEDGENKL
                     NSGIKGVQTGIDNMVKARAGINQGIDGVKKGIAGVDKGIKGMEQGLEKQDEAIKELTT
                     AYNKIPTTEKTPTDNENNSNEQNQSNTSNSESDDTKNDSTENKNNNSSENKPNVNEQK
                     ESLNAQIQQLKKTRDDLNTKLQKNKTQKNELSKKLRTMESQKKELQSKLNNSISQKKD
                     MEKSLDTMQQQKKSLQAVLAKTQEVKTEIPKAFDKSKLDYINSIENNRTKIENTLQST
                     LNSGFKQMYITVFCVNLLAFIILLFYKENKTR"
     gene            complement(2507..3049)
                     /locus_tag="PC414_002178"
     CDS             complement(2507..3049)
                     /locus_tag="PC414_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2464573.1"
                     /translation="MPKILENVKEDILKVSRDMILEEDYSNISIRKIAQRCGISAGTV
                     YNYFNSKQEIIEYITKSEWDLLIRRIEYSNKNTEDYIKKLNIIFTEIRNFINKVHNIQ
                     YNDFLNTFETERFFEMKKHKDDFHEQLSNKVYEALEKESFFNNDKLVCNIIIKMFFSY
                     STYLHIEFEDLKPYIEKLIH"
     gene            3293..4807
                     /gene="glpK"
                     /locus_tag="PC414_002179"
     CDS             3293..4807
                     /gene="glpK"
                     /locus_tag="PC414_002179"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDF2464574.1"
                     /translation="MEKKYVMALDQGTTSSRAILFNKKGEIVKIAQKEFNQIYPKAGW
                     VEHDPMEIWGSQSGVMREVIETAGIRPEEIASIGITNQRETTVVWSRYTGKPIYNAIV
                     WQCRRTSEICDELKNKGLEESIKEKTGLLIDAYFSATKVKWILDNVEGAREKAEQGEL
                     LFGTIDTWLIWNLTRGKVHVTDYSNASRTMMYNINDLEWDEDILRELDIPISMLPLVK
                     PSSYVYGHTDERMLSGAKIPIAGCAGDQQAALFGQNCVEEGTAKNTYGTGCFLLMNTG
                     SNIVKSKHGLLTTIAWGVDGKVTYALEGSIFIGGASVQWLRDELKIIESAKDSEMYAN
                     RVEDTNGVYVVPAFTGLGAPYWDMYARGSILGLTRGAKKEHIVRATLESIAYQTKDVL
                     EAMQNDSKLKLKSLKVDGGASNNNFLMQFQSDILNVDIDRPKIVETTALGAAYLAGLS
                     VGFYIGRNEITSKWSVEKEFNPNMSEEKRCKLYKGWKKAVSRALSWEKEDELDI"
     gene            complement(4921..5550)
                     /locus_tag="PC414_002180"
     CDS             complement(4921..5550)
                     /locus_tag="PC414_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2464575.1"
                     /translation="MDKNYTMPIYQKIALDIANKIYTGEIQEDSVLFGRSVLAGKYNV
                     SPETIRRAVKILEDIGVVKSIKGKGVIVLSPDKASSFIKKYRDITNISSYKSTLYNLI
                     DTKSNLENEILDTINKILDYSNRLEIINPLVPVQFTINSNCKYIGQTAAQTKFWQNTG
                     ATIVAIKRGEELIISPGPYIEFLEGDILLVVGDQHIYNSIPMFLYENEK"
     gene            6138..6929
                     /locus_tag="PC414_002181"
     CDS             6138..6929
                     /locus_tag="PC414_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HDF2464576.1"
                     /translation="MYKVDLNSDLGESFGTYKIGLDEEVLKYISSANIACGFHAGDPS
                     HMEKTVQLAKKNGVKIGAHPGFLDLIGFGRREMKITKQEAKDYTKYQLGALMAFASSN
                     GCNIQHVKPHGALYNMAAKDKELAMGICEAIYEVDKDIILLGLYNSEMINSAKEIGLR
                     FANEVFADRAYDNNGFLVPRNVEGAVIHDTKHAIDRVVRMVKEGTVETLTGEVIHIKA
                     DSICVHGDNPKAIEFVKEIRKRFELEGIEVCPLENIEVCSSENIV"
     gene            6958..8178
                     /locus_tag="PC414_002182"
     CDS             6958..8178
                     /locus_tag="PC414_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HDF2464577.1"
                     /translation="MSTKNITDKTKNKKDVGALIGAAFIMATSAIGPGFLTQTAQFTQ
                     DFGPNFSFVILITTILFIGAQVNVWRVIGVSGLRGQDIANKIIPGLGYLVAFLVALGG
                     LAFNIGNVGGAALGMNVMFNMNMTLGTVLSGLIAIFVFMSKNSNSLVDKITKFLALGM
                     IIIVGYVAISNHPPVGEAVSRMVKPENPKGLIFPIITLLGGSVGGYITFAGGHRLIDG
                     GITGEENIKEITKSSLLGILVATMMRVLLFLAILAVVSKGLQLDPENPAASAFKFSAG
                     AIGYKFFGLVLWSAAITSVIGAAYTSVSFLKTLNPFIDKYEKYFIIAFIAISTLIMAF
                     IGKPATLLILAGALNGLILPITLGIMLIASKRKDIVGDYKHPTWLLIFGLIVVLISAY
                     TGITSLSSLGALFA"
     gene            8198..8893
                     /gene="pxpB"
                     /locus_tag="PC414_002183"
     CDS             8198..8893
                     /gene="pxpB"
                     /locus_tag="PC414_002183"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HDF2464578.1"
                     /translation="METRYLISGDKAVVAEFGNEISEDINKKVISFMRAIEISNLKGI
                     VTEMVPTYRSLMISYNPLEIDFDSLIENLKKIEDNLESIVLPKPKIHEIPVCYDEVFG
                     IDIKNVASYNNLTVDEVIKIHTSREYLIYMLGFTPGFPYLGGMDERIATPRLEVPRTK
                     IYGGSVGIAGSQTGVYPIDSPGGWQIIGRTPLKLYDENREEQILLRAGDFIKFVPITL
                     DEFIEIEKNNLSI"
     gene            8923..9909
                     /locus_tag="PC414_002184"
     CDS             8923..9909
                     /locus_tag="PC414_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase"
                     /protein_id="HDF2464579.1"
                     /translation="MGFKVELGGFQTLIQDRGRVGYGQYGVSGCGAMDEYAHRVGNIL
                     VGNSEDEASLEVLMLGPTITFDEYTQIAVTGGDLGAKINGKEIQNWRSYQINPGDVLS
                     FRGVKSGTRAYVSIAGGIDVPLAMGSKSTYTRAKIGGFEGRALKKGDYINTFIQEKDF
                     TINKKLSSKYIPTYSSEIVLRIVKGPQFDAFSNGEVEKFLSNKYKVTNEIDRMGCRLD
                     GESIKHLNGADIISEGISYGAIQVPGHGKPIIMLSDRQTSGGYTKIGNVISVDLYKLA
                     QAKPNDVVKFELVDIYEAHRLLREQEDKIQDIYKSMKNIRVVKAKVLNDIAV"
     gene            complement(10123..10689)
                     /locus_tag="PC414_002185"
     CDS             complement(10123..10689)
                     /locus_tag="PC414_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2464580.1"
                     /translation="MLGEKMRNIRKSKKKTLSDVSKLTDLSISYISQIERDAIEPSLS
                     SLRKIAEVLDTPLYMFMDDNNTDDLVIRKEDRVMMKFPKSEMFYEIVSPMPTTEFTPS
                     ILFVEFELKPESEDAKDYIYHASEEIMVVTSGVVDICMGEKVVRLNPGDSTFIKANVP
                     HKIINPSKDTIARGYGVISPPIWPIKSK"
     gene            11041..12108
                     /locus_tag="PC414_002186"
     CDS             11041..12108
                     /locus_tag="PC414_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HDF2464581.1"
                     /translation="MKITDIKFEKLRIKLKKPVVVSFGVIEYGESIILKIETDEGYCG
                     FGEAAPLAAVTGEVLDNVLSVLLMFKKELIGKDPLDIETIHTIMDGIIIGNTSAKAAI
                     DIALYDIKGKIMNAPLYKVLGGFDSKVQTDITISIDKPEKMAVEALERVKEGFRILKL
                     KAGINPEDDIEAVKLIREAVGESIRIRIDANQGWNVNSSINTIKKLEEFNVDAIEQAL
                     PHWDLDGTAYIRNKSNTKIMIDESLHSPIDAIKAIKKNAVDTFNIKLMKSSGIYPAIK
                     INNIAEASGVNCMLGCMLETRIGITAAANLIASKKNITEADLDSFMFCEELEGISGGF
                     VMDGDIMNLVNKPGLGIEVNL"
     gene            12127..13287
                     /locus_tag="PC414_002187"
     CDS             12127..13287
                     /locus_tag="PC414_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HDF2464582.1"
                     /translation="MNTLFSNPGSILAVMTSMIALGFYLQRYKAIKSLGPALTIIIMG
                     IILSNLKVVPVSTELYGTISTYAIPVSMTIMLMSVDLKEMTKLSREPLIAIFVAVLTV
                     SIMAFLFGLVFAEKISEGWKVAGMFVGTYTGGSANLTAIGTGLNVSRQTLAAANAADY
                     VIGVPTLIFMFALPAILKNSKKFKKLWPYHVEESELEDCQNEEFMESKEWSIKDIAWM
                     LAIGFVVTEVATILAGYFNSSFSSAARILLVTTISIIIAQLKPVKKLKGNLDLGLFVA
                     LFFLCTIGFSVDIKEFLGSTFTITLYCFSIIFASFVFHLGITRLLKIKYQYVILSIVG
                     AIADGPTSALVAASAKWNSLVSVAVVMGVIGGVLGNYAGISVAYAIKMSLGL"
     gene            13300..15396
                     /locus_tag="PC414_002188"
     CDS             13300..15396
                     /locus_tag="PC414_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HDF2464583.1"
                     /translation="MIKFKLYISGLYSGNVIFDGDLLIEKLNPFTNKIESLKPISKEE
                     NTYYLNLTKIDLKSLFNNFDVYTKSLVNTDKDTVINNLGETHSKLNEYIWVQRNKKFP
                     LDIIIVDNKIVGFICLSRETCTILIMDGYEEYTVLKEWEKTHKNEEIYSIRFGGNYMI
                     DMKDGIKLSTDVYLPDFVDSTKKAPTILMRTPYGKENDKEIYYKYVQRGYAVVIQDVR
                     GRNESEGKWEPMIHEREDGDSTINWIVSQEWSSGIVGMLGASYLGYVQWAAASSGNKH
                     LKALVSIVTSGSPFIDIPRKGGAFVSGMLAWAFMVSRNKVDRSKMVRDDWDDVLNIRP
                     IENIPVEALGYRIEFLEEWLKRVEKDEYWDLMDWHLQKDKINVPALVVSGWYDDNSMG
                     TTEALDVIKDYEKGKRKAILGPWMHNSNTLRDINGISLGNSSLRYDLDYNYLLWFDKY
                     LKGIENNIDTTAPVEYYSVGSNKWKTEENWPIINKIDKSMYLISDGNANTSLGNGRLV
                     FDNGLEEKYDSYIYNPKDPSVQLIDMSENEVGVPNNYKDLEKRSDMLCYTSDAFSEEF
                     TVTGDIKLEFFASSSAKDTDWVIKIMDVDLDGNSIKLADGILSARFRNSFYKSEFMEE
                     GEIYKFTIITSKISNTFKVGHKIRLDITSSAKNFIFQNSNTTEGYNSIEYIEAKNTIY
                     HGGKYPSKLILPIENK"
     gene            complement(15555..15899)
                     /locus_tag="PC414_002189"
     CDS             complement(15555..15899)
                     /locus_tag="PC414_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDF2464584.1"
                     /translation="MEIKRYEGTGRMSRAVVHNNTVYLCGQTHAEGGVIEQTTEVLAK
                     IEDLLNKYGSDKQHLLSVTIYLRDMKDFEAMNSVWDAWVEKGFEPARACVEARLAREH
                     LLVEMSVVAATK"
     gene            16191..17081
                     /locus_tag="PC414_002190"
     CDS             16191..17081
                     /locus_tag="PC414_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDF2464585.1"
                     /translation="MSSTIKSNRIKGIMFIIASALGFAMMSAFVKLAGDLPSFQKVFF
                     RNLVSAIIAFWLIIKHKGSLTGKKENLKVLLYRSIFGTLGVIFNYYAIDKLMLSDANM
                     LNKMSPFLVVIFCAIALKEKINVKQIGAIIIAFIGALFIIKPTFSVEIIPYLGGIAGA
                     VFAAMAYTCLRVLGDREDYYTIVFFFSVFSLVTVGPIAFAVYEPMTLMQLIYLLLGGV
                     FASLGQFGITLAYKYAPAKEISIFDYSNIIFSAILSIFLFNVYPDMLSVVGYLIVFSA
                     AFYMFLYNKKLDKLDKEKKK"
     gene            17299..18585
                     /locus_tag="PC414_002191"
     CDS             17299..18585
                     /locus_tag="PC414_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDF2464586.1"
                     /translation="MEKVKVAVIGAGNRGTYAYAPYIYENSDVCEIVAVAEPKKGRRE
                     LFTQKYNLDSKNVFETLEDFFKHDKMADAVIIATNDDRHYDVAKLALEKGYHVLLEKP
                     MSNSLDGLVHIDDLCDKYKDKIFMICHVLRYSPFYNKLKEIVESKKLGELVSIQYNEN
                     IGYWHFAHSFTRGNWRNSNETSPLILAKSCHDMDILLYLVGSRCKKISAFGSLKHLNN
                     QNASGEMAQNCLQCLVEKKCPYSAKRIYLEKDRSINRAVHINPTEENLLNILKTSPYG
                     RCVYRCDNNVVDNMVNILQFENGVTATFNLCAFTKENGRTIKLMFSHGEVGGDLNKNE
                     IRIKEFGKNEEIVMNPSNQQNMVEEYDRNLIAEFIKLVSNKELEKGRVAAKEAIQSHV
                     MAFAAEYSRVSDEVVYIEEFFDSAKQMTKEIEETIF"
     gene            complement(18674..19117)
                     /locus_tag="PC414_002192"
     CDS             complement(18674..19117)
                     /locus_tag="PC414_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory YrvL family protein"
                     /protein_id="HDF2464587.1"
                     /translation="MIKISNKRRIPLGEFISVFLFFAVFFGITFCIFTVIGIGFLSFL
                     GFEYKSLGAVLIFFLIYFCITTPIDFLCTTILDIFRYVNKLPYSIYKLCEFIIDFILT
                     FLAMNIIDTFMDSVTIPLSTEILFALLSYLLSECMDFFDRDKKKP"
     gene            complement(19141..19935)
                     /locus_tag="PC414_002193"
     CDS             complement(19141..19935)
                     /locus_tag="PC414_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HDF2464588.1"
                     /translation="MKLYLSADIEGTCGIVNWDETKLECEFSQHYRTQMSKEITAACD
                     GATQSGVHEILVKDAHDSGRNINPNILPENVKILRGWTKDPLVMMAGIDESFDACIFT
                     GYHSGATCNGNPLSHTMDIDYDYFKINDEIASEFTINAYIAAYYNVPVAFLSGDEMLC
                     ESAKKLNPNIVTVPVSKGIGNGSISIHPNLALKKIEKGVKKALSGDLSRHLIKLPDKF
                     KIEIKFREHYKAFKASFYPNMKKIDSQTVLFETEDYYEFLRMLLFI"
     gene            complement(20500..20976)
                     /locus_tag="PC414_002194"
     CDS             complement(20500..20976)
                     /locus_tag="PC414_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HDF2464589.1"
                     /translation="MKEILINPIGKICIQGEEVFIKLDKKYVPALNELDDFSHLNVFW
                     WADGFDNPELRSILETPKPYKSGPDVIGIFATRSPIRPNPIALTAVQIINIDHENGII
                     KIPYIDANDNSPVIDLKPYTPSVDRVENPSIPKWCSHWPKSVEKSGEFNWEDEIEF"
     gene            complement(21077..22246)
                     /locus_tag="PC414_002195"
     CDS             complement(21077..22246)
                     /locus_tag="PC414_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2464590.1"
                     /translation="MLNIKTLCHEINDWVINIRRDLHKTPELGLEEFQTKKKIIKYLN
                     EIGINYIEYKNHTGITAYINVSPNFETVAIRADIDALPITEELNYSYKSINIGKMHAC
                     GHDAHTAILLGTCNILFKLKDYLNVNVKFFFQPAEETIGGAQLMIEDGCLENPNVKYI
                     FGLHVNSNINKNLIELKYNTLNASTDTLQLTVHGSKCHGAYPHQGIDAIVISAHIITA
                     LQTIVSRNTNPTDSVVISLGKIEGGIKENIVCDKVVIRGTLRTLTPETREFSKKRIRE
                     ICDFTCKTFGGSISVEIEEGYPALINSNHLVDYVKQNAVELFGEENIILKDSPTLGAE
                     DFSYFLRHCEGAFYHLGCANREKNITSPLHTSTFDIDEDCLITGVMLHVKNVLSF"
     gene            22409..23272
                     /locus_tag="PC414_002196"
     CDS             22409..23272
                     /locus_tag="PC414_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HDF2464591.1"
                     /translation="MLGIIAPSGPLRNTSLEEIKFNLESYGYEVKFSESCSLNYKGYL
                     AGNDDIRARDIEDMFLDKDVDIIMCLRGGYGTTRILDKINYDIIKQNPKPFIGFSDIT
                     GLNLAFYKNCGLLPYHGIMAANVGKWDKFTYKSLVNALEFKDELYLENPKEEKIYTVC
                     EGKAEGIIMGGNLSLIIATMGTKYEIDAKDKILFIEEIGEPQYKLDRMLTQLYSSGKL
                     EECNGIIFGDFKDCIEENDLMELLIEFANKVNKPSIYNLQSGHCIPMITIPLGRMCEL
                     DATEKIVKLKK"
     gene            23343..23936
                     /locus_tag="PC414_002197"
     CDS             23343..23936
                     /locus_tag="PC414_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3867 domain-containing protein"
                     /protein_id="HDF2464592.1"
                     /translation="MSDDRIIDFNELKNKVKDSDVDKFEQYIYNLYFSVMDGKMSMAE
                     FSRKIFDYMRDNNISQEKFMKIQKQFMERYGMDTEEVEKQLRNFGIDPSTAGFMSNNT
                     SSKVSTEDLESFKKSAGFYEKYGEKIQPKSCITTFIKNDLNDINVIIDQEKIMLCSDR
                     KINLMDSELNEFLLEYKNMFNKKIKVVMCETTNKYDY"
     gene            complement(24187..24822)
                     /locus_tag="PC414_002198"
     CDS             complement(24187..24822)
                     /locus_tag="PC414_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDF2464593.1"
                     /translation="MDNLLNELEALKSNLDNLPIEKIENYDLSKTALFIIDVNNGFAR
                     QGALYSPRVESLIKPIEMFTKKISNKLNRVIAFTDSHTPKSIELLSYPVHCLENDVES
                     ELVDELKSIENLQILPKNSTNGFFALENLDFDNIDNIIIVGDCTDICIYQFAITLKSY
                     FNQHNIEKNIVVPMNLVDTYDIPNVHPAEILNLVFFNSMIQNGVNVLKEIR"
     gene            complement(24902..25633)
                     /locus_tag="PC414_002199"
     CDS             complement(24902..25633)
                     /locus_tag="PC414_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDF2464594.1"
                     /translation="MNIYAHRGFSGKYPENTILAFKKCLDMDIYGIELDVHRTKDGKI
                     VVIHDEKVDRTFNGHGFVKDFTLRKLKTLNSSFEGYQSNKECKIPTLEEVLILISPTD
                     LTLNIELKTDKINYPNIEKGVLELILKYNMKNRVLISSFNSNSLKNFHKLDPSVKTGL
                     LCYLPINNVVNFAKFLGNSYLHPPLVLVNESLIELCHKNLLGVNVYTVNEEDDILHCL
                     KLNVDGIFTNYPDIASNLLHSKQYS"
BASE COUNT         9537 a         3126 c         4205 g         8832 t
ORIGIN      
        1 ttatctagtt ttattttctt tatagaacaa taatattata aatgctagta gatttacaca
       61 gaatactgtt atatacatct gtttaaatcc tgagtttaga gtagattgta gtgtgttttc
      121 tatttttgtt ctattatttt ctatggaatt tatataatcc aattttgatt tatcaaaagc
      181 ctttggtatt tcagttttta cctcttgtgt ctttgctaaa actgcttgga gtgatttttt
      241 ttgctgttgc attgtatcaa ggcttttttc catgtctttc ttttggctta tagaattatt
      301 taacttgctt tgtagctctt tcttttgaga ttccattgtt cttagttttt tacttaattc
      361 gttcttttga gttttatttt tttgtaactt tgtatttaag tcatctcttg tcttttttag
      421 ttgctgtatt tgagcattta aactttcttt ttgttcattt acatttggtt tattctcgct
      481 ggaattgtta ttcttatttt ctgtagaatc attcttagtg tcgtctgatt cagaattact
      541 ggtattactc tggttttgtt catttgagtt attttcgtta tcagtaggag ttttttctgt
      601 agtaggtatt ttattataag cagttgtaag ttccttgatg gcctcatctt gtttttcaag
      661 accttgttcc atacctttta tacctttatc aacaccagca ataccttttt ttacaccatc
      721 tattccttga ttaataccag ctctagcttt taccatattg tcaataccag tttgaactcc
      781 tttaatacca ctatttagtt tattttcacc atcttcaata ccgtttattc cactttgtgt
      841 tccatctatt cctttttgta catttttctg tattttagta attatagatg gaactttttc
      901 atcaaacatt gttgttgata tttcttttgt tatattggta atgttggttg tatctgctga
      961 ctgaacttta ttaagtaagg attttggaag tttgccacct gtcatgtcca tatttatatt
     1021 tgaagtatca ttcatatttg ggatagagac attttttaaa ttttttgcca tttcagggtc
     1081 tgattttaag tcatctatca tgttttcaag ttcttcaagt tgaactattt taggagttga
     1141 gggctttcca actaagtcca taatattatc ttgcacagat aatcctgcat gtgctataaa
     1201 tccaatcatt atagctggag aaattgaagt accaattgag cgtatcaagg ataatgttgc
     1261 aagtgctgaa ttagattctt ctaatcttgt atttgataac atcatatagt ttagaggagt
     1321 tcccatagta aatcccattc ctaatcccat aaatattaaa cttacacata cactaaatac
     1381 agtattggtt tttagagcaa ttaaaactag gtaaagagag cctatcatag atacagaaaa
     1441 tcctagtaat agtatttttt ttgccccaaa cttgtcaata agtcttccgc ttaaaggagc
     1501 agcaatacct gcaaataatc ccaatatagc tacaaaatat cccccagaac cagcatttat
     1561 ctttaatgca ttttcagcat actgtggaac gaataccata cccatcattc caataccaac
     1621 aattaaagat aaaataagta ctatcaatat tctcggattt aaaaaatatt caaagcttaa
     1681 aatagggtct ttagctctat tttcaataaa tatgaaaata ggtataagta caataaatgt
     1741 caataagtat ggataaactg atatatcttg tatagaattt ttaaaattaa aaaagtctat
     1801 attcataaga ccatataata gtgatacgat tatacatatc aacatcaatg taccaagctt
     1861 atctattttt tctttagatt cagacttatt gtttttaata cagaaaactc caccaataag
     1921 tatgattaaa caaattggaa gatttacaaa aaatagccac ttccaatttt gagttccaaa
     1981 gatacttaga attgtgctac caatgcttga acctaaaata ttggcaatac catatgttgc
     2041 cccaactaaa cctaaagcca tgcctctttt attttctggg aaggtagttc caaattctgc
     2101 tgtagcaatt ggcatgattc caccaccacc aattgcttga ataacacgac caattaataa
     2161 aatatagaaa ttggaaaata gtgatgataa tccacatatt aatgaaccac taccaaatag
     2221 aaatatacta actagataca catattttct tcctatttta tctgctaatt ttccagagat
     2281 aggaattaca gaagcgtatg ttaatgtgta tatggtaatc atccaaattc ccgttttttc
     2341 atttactcca aggctatttt gtataatcgt tcttgctggt gatactattc cagtatcaat
     2401 agctcccata aatataccta atagataaag cgtaagtact aaaaatattt ttggattact
     2461 gtccttgttc ttaatactag tattatgatt caaggtaaac accaccttaa tgtattaatt
     2521 tttcaatata gggttttaaa tcttcaaatt ctatatgaag atatgttgaa tatgaaaaaa
     2581 acatttttat tatgatattg catactaatt tgtcattatt aaaaaaactt tctttttcta
     2641 aagcttcata tactttattt gaaagctgtt catgaaagtc gtctttgtgt tttttcattt
     2701 caaaaaatct ctctgtttca aaagtgttta aaaagtcatt gtattggata ttatgaactt
     2761 tgtttataaa atttcttatt tcagtaaaaa taatgtttag tttttttatg taatcttcag
     2821 tgtttttatt ggagtattca attcgcctta tcaataaatc ccattcagat tttgtgatat
     2881 attctattat ttcttgtttt gaattaaagt aattatacac tgtacctgca cttataccac
     2941 acctttgagc tattttcctt atgctaatat tcgagtaatc ttcttctaaa atcatatccc
     3001 gagacacttt taatatatct tcttttacat tttctaaaat ctttggcata ttaccacctc
     3061 ttttattaaa attattatga acactgttta ttatactcct ttaaaattta ttaatcaata
     3121 tgtaagctat aatgtataat attattatat agatatgata gaaaataagt gtcttcttac
     3181 taaatatcta ttatattaaa tttgtactta agtatgatta tttattgaaa tatgtgatat
     3241 taaatttttc tttttaataa attaaatatt atatgataag gagaatatgg tgatggaaaa
     3301 gaagtatgta atggcattag accaaggaac aaccagttca agagctatac tatttaataa
     3361 aaaaggcgaa atagtgaaaa ttgcacaaaa agagttcaat caaatttatc caaaagctgg
     3421 atgggtagag catgacccta tggaaatatg ggggtcacaa agtggggtaa tgagagaagt
     3481 tatagaaaca gcaggtataa gaccagaaga aattgcttca atagggataa ctaatcaaag
     3541 agagactacg gttgtttgga gtaggtatac tggtaaacct atttataacg caatagtatg
     3601 gcagtgtagg agaacgtcag aaatatgtga tgaacttaaa aataaaggat tggaagaatc
     3661 aataaaagaa aaaacaggtc ttttaataga tgcatacttt tcagcaacaa aagtaaaatg
     3721 gatattagat aatgtagaag gagctaggga aaaagcagaa caaggagagt tattatttgg
     3781 aactatagat acatggttaa tatggaattt aactcgtgga aaagtacatg taacagatta
     3841 ctcaaatgca tctagaacaa tgatgtacaa tataaatgat ttagaatggg atgaggatat
     3901 attacgagaa ttggacatac caataagcat gttgccatta gtaaaacctt ctagctatgt
     3961 ttatggtcat acagatgagc gcatgttatc aggagctaaa atacctattg cgggatgtgc
     4021 tggagaccaa caagcagcat tatttggtca aaattgtgtt gaagaaggta ctgcaaaaaa
     4081 tacttatgga acgggttgtt tcttgcttat gaatacaggc agtaacatag ttaaatctaa
     4141 gcatgggtta cttacaacaa ttgcttgggg agttgatgga aaagtaacat atgcattaga
     4201 aggaagtata tttattggag gagcatctgt tcaatggctt agagatgaat taaagataat
     4261 tgaatctgca aaagatagtg aaatgtatgc aaatagggta gaggatacta atggtgtata
     4321 tgtagtgcca gcatttacag gtcttggagc tccatattgg gatatgtatg ctaggggttc
     4381 aattttagga cttacaagag gagcaaaaaa agaacatata gtaagagcca cattggaatc
     4441 aattgcttat cagacaaagg atgttttaga agcaatgcaa aacgattcaa aattaaaact
     4501 aaaatcatta aaagttgatg gtggagcaag taataataat tttttaatgc aatttcaatc
     4561 agatatatta aatgtagata tagatagacc caaaatagta gaaactacag ctttaggagc
     4621 tgcttattta gcagggttgt ctgttggatt ctatattgga agaaatgaaa ttacttcaaa
     4681 gtggtcagta gaaaaagagt ttaatccaaa tatgagtgaa gagaagagat gtaaattata
     4741 taaaggatgg aaaaaagctg tatcgagagc tttaagctgg gaaaaagaag atgaattgga
     4801 tatttaaaaa ttaatcaaag ttaaatttat ttgaatttaa aaagagcttt atgtaaaata
     4861 tgaagctctt tttttgttgt taatcttgca tttttatata atcacactaa attatataat
     4921 ttatttttcg ttttcatata aaaacattgg tatagaattg taaatgtgtt ggtcaccaac
     4981 tactaagaga atgtctcctt ctaaaaattc aatatatggt ccaggagata ttataagttc
     5041 ttcacctctt ttaattgcaa ctattgtagc acctgtattt tgccaaaatt tagtttgtgc
     5101 agctgtttga ccaatatatt tgcaattaga atttatggtg aactgaacgg gcactaaagg
     5161 attgattatt tcaagtctgt tagaatagtc taaaatttta tttatagtat ctaatatttc
     5221 attttctaaa ttagatttag tatctattaa attataaagt gttgatttat atgatgatat
     5281 atttgtaata tctctatatt tttttataaa agaagaagct ttgtctggag atagtacaat
     5341 aacaccttta ccctttatgc ttttaacaac gccaatgtcc tctaaaattt tgacagctct
     5401 tcttatcgtt tctggagata cattgtattt accagccaaa acagagcgtc caaatagtac
     5461 tgaatcctct tgtatttcac ccgtgtatat tttatttgca atatccaacg ctattttttg
     5521 gtatattggc atcgtatagt ttttatccat tgtttttccc ccaatacaaa atttatgtta
     5581 aaaaaatata taatcttaac aatactattg tataatttat aggtttatat gtctacaata
     5641 tgtacaaata ttgtgcatgt tttaaaataa tattttaaca tgcatttagt atacatgatt
     5701 ttattatact taaaactata taaagaatta attataaaaa tataaagata ttttacttac
     5761 aaagaatgat aattatatag atgaaagaaa caaatgaatg aagaaaaatg attctgtctt
     5821 gcatataaag tgattaaaaa tgaaaaatac tttaaaaatg aaggttaaag cccttatttt
     5881 aagtgaaaat aattaattat taactaaaaa atggtaaaaa gattttttga aaaagaaata
     5941 caaatgaatg ttcaattaaa ataattgcaa aaatattcct taaatggtaa aattatatta
     6001 attaaatgca atagaatagt tgcaaaaata aaattgaata caagtgataa aatatttcat
     6061 gcaatatata aaataaacaa aatttaaaaa ttaaaactac ttattctatc aaaatgttag
     6121 cgaggagggg tcataaattg tataaagtag atttaaatag tgatttagga gaaagttttg
     6181 gaacgtataa gattggttta gatgaagaag ttttaaaata tatttcatca gccaatatag
     6241 cttgtgggtt tcatgcaggt gaccctagtc atatggaaaa aacagtacag ctagctaaaa
     6301 aaaatggagt gaaaataggc gcacatccag gtttcctaga tttaatagga tttggaagac
     6361 gtgagatgaa aataacaaag caggaagcta aagattatac aaaatatcaa ctgggtgctt
     6421 taatggcatt tgcaagttca aatggatgta atatacaaca tgtaaaacca catggagcat
     6481 tatacaatat ggcagctaaa gataaagagt tagctatggg gatttgtgaa gctatatatg
     6541 aagtcgataa agatattata ctgttggggc tatataatag tgaaatgata aattctgcta
     6601 aagaaattgg acttaggttt gcaaatgaag tatttgcaga tagagcttat gataataatg
     6661 gttttttggt tccaagaaat gtagaaggag cagttataca tgatacaaaa catgcaatag
     6721 atagggttgt taggatggta aaagaaggaa ctgtagaaac tttaacaggt gaagttatac
     6781 atatcaaagc agattctata tgtgtacatg gggacaatcc aaaagcaata gagtttgtca
     6841 aagaaataag aaagcgtttt gaattagaag gtatagaagt ttgtccatta gaaaacatag
     6901 aagtttgttc gtcagagaac atagtataag actgataaca tgagggggag ataaaatatg
     6961 agtacaaaaa atattacaga taaaactaaa aataaaaaag atgtcggagc acttataggg
     7021 gctgcattta taatggcaac atcagcaatt ggaccagggt ttttaactca gactgcacaa
     7081 tttacccaag attttggacc gaatttttca tttgtaattt taattacaac aatccttttt
     7141 ataggggctc aagtaaatgt atggagagta attggtgtat caggacttag aggtcaggat
     7201 atagcaaata aaataatacc tggattaggc tatttagtag cttttttagt agctcttgga
     7261 ggattagctt ttaatatagg aaatgttggt ggagctgcac ttggtatgaa tgtaatgttt
     7321 aatatgaata tgacattagg aactgtatta agtggattaa tagctatatt tgtatttatg
     7381 tcaaaaaatt caaattctct agtcgataag ataactaaat ttttagcatt aggaatgata
     7441 attatagttg gatatgtagc aatatcaaat catcctcctg ttggagaggc agtatcaaga
     7501 atggtaaaac cagaaaatcc aaaaggactg atattcccaa taataacttt acttggagga
     7561 agtgttggag gttatataac ttttgctgga ggacatagat taatagacgg tggaataaca
     7621 ggagaagaaa atataaaaga aataactaaa tcttctcttt tgggtatatt agttgccaca
     7681 atgatgagag ttcttttatt tttagcaata ctggcagtgg tttcaaaagg tcttcaactt
     7741 gacccagaaa atccagcagc ttcagctttt aaatttagtg caggagcaat aggatataaa
     7801 ttctttggac ttgtattatg gtctgctgca attacatcag ttataggagc tgcttataca
     7861 tcagtttctt tcttaaagac actaaatcct tttatagata aatatgaaaa atattttata
     7921 atagcattta tagcaatatc aacacttata atggcattta taggaaaacc agctacttta
     7981 ttaattttag caggagcttt aaatggattg atacttccaa tcacattagg aattatgctt
     8041 atagcatcta aaagaaaaga tattgttgga gattacaagc atcctacatg gcttttaata
     8101 tttggattaa tagtagtttt aatatctgca tatacaggaa taacttctct tagcagccta
     8161 ggtgctttat ttgcttaatt tagaagaggt gacatttatg gaaactagat acttgatatc
     8221 aggggataag gcagtggttg ctgaatttgg aaatgagata tcagaagata taaacaaaaa
     8281 agtaatttca tttatgagag caatagaaat atcgaatctt aaaggtattg taacagaaat
     8341 ggtaccaaca tataggtcac ttatgataag ttataatcca ttagaaatag attttgatag
     8401 tttgattgag aatttaaaaa aaatagaaga taatttggaa agtatagtat taccaaaacc
     8461 taaaatacat gagattccag tatgttatga tgaggttttt ggtatagata ttaaaaacgt
     8521 tgcaagctac aataatctca ctgtagatga agttataaaa atacacacaa gcagagagta
     8581 cttgatatat atgctagggt ttactcctgg atttccatat ctaggtggta tggatgaaag
     8641 aatagctaca cctagattag aagtgccaag aactaaaatt tatggtggga gtgttggaat
     8701 agcaggttcc cagactggtg tatatcctat tgatagtcct ggtggatggc aaataattgg
     8761 taggacacct ttaaaattat atgatgaaaa tagagaagaa cagattttgc ttagagctgg
     8821 agactttata aaatttgttc caataacatt agatgaattt atagaaatcg aaaaaaataa
     8881 cttaagtatt taaatatcgt tatagtacag ggagtggtat atatgggatt caaagttgaa
     8941 ttaggaggtt tccaaacatt aatacaagac agaggtagag ttggctatgg tcaatatggt
     9001 gtatctggat gtggagcaat ggatgagtat gcacatagag taggaaatat attagtagga
     9061 aattctgaag atgaggcttc tttagaagtt ttaatgttag gtcctacaat tacctttgat
     9121 gaatatacgc aaatagcagt aactggggga gatttagggg ctaaaataaa tggaaaagaa
     9181 attcaaaact ggaggtcata tcaaataaat ccaggtgatg tattatcatt tagaggtgtg
     9241 aaatcaggta ctagagccta tgtgtcaata gctggtggaa tagatgttcc acttgcaatg
     9301 ggaagtaagt caacttatac aagagctaaa attggtggat ttgaaggtag agcacttaaa
     9361 aaaggcgatt atataaatac ttttattcag gaaaaagatt ttacaataaa taaaaaatta
     9421 agttcaaaat atattccaac ttatagttca gaaatcgtac taagaattgt aaaaggtcct
     9481 caatttgatg ctttttctaa cggtgaagta gagaaatttt tatcaaataa atacaaagtt
     9541 acgaatgaaa tagatagaat gggttgtaga ctagatggag agagtataaa gcaccttaat
     9601 ggagcagata ttatatctga gggtatttct tatggagcta tacaagtacc tggtcatgga
     9661 aaacctatta taatgttatc agatagacag actagtggtg gctatactaa gatagggaac
     9721 gttataagtg tagatttgta taagttagca caagcaaagc caaatgatgt agtaaaattt
     9781 gaacttgtag atatatatga agcacataga ctattgagag agcaagaaga taagattcaa
     9841 gatatctaca agagtatgaa aaatataaga gttgtaaagg caaaagtatt aaatgatata
     9901 gcagtttaat tttaacaaat acaattttta ttatagttta taatataact tacaaatagc
     9961 tatttactat ttaccataat taagatggtt tagattagtc aaatagctat ttttttagtt
    10021 tgagtaaata ttttaaattt aaataaatac ttcaatttta aaataaaagc aatatttaaa
    10081 tctattatat tatctacaaa aagtaggtgt gtcatattga gtttatttag attttattgg
    10141 ccaaattgga ggtgatataa ctccatatcc acgagctatt gtatctttac ttggattgat
    10201 gattttatgt ggtacattag cttttataaa tgttgagtca cctggattta atctaactac
    10261 cttttcaccc atacaaatat caaccacacc actcgttacc accataattt cttctgatgc
    10321 atgatatata taatctttgg catcctcact ttctggttta agttcaaact ctacaaataa
    10381 aattgaaggt gtaaactcag ttgtaggcat tggagatact atctcgtaaa acatttcact
    10441 ctttggaaat ttcatcataa ctctatcttc ttttctaatg actaaatcat cagtattatt
    10501 gtcatccata aacatatata aaggagtgtc taaaacttca gcgatttttc tcaatgaaga
    10561 caaggatggt tcaatagcat ctctttctat ttgagagata taacttatag ataaatctgt
    10621 caatttggaa acatcactta atgttttttt cttgcttttt cttatatttc tcatcttttc
    10681 acctagcata agaacctcca aataataaaa ttaatataaa atattcgctt aaaacaaagt
    10741 aattatagca caacatagaa gtagtggcaa taagaataca tagtagattt tgacaattaa
    10801 aatttaatta aatccaaata aaagtctatg aaaatgaatt attgttaaat taatacaaag
    10861 aaaataataa taaaaaaatt taatagaaga gtattaattg aattaagatt atcacattat
    10921 attttggaaa agagtaaatt ttcagtaaat tatcttgaca tatatattga agtaataata
    10981 taatttaaag tataaaattt taaaaattga attataactt caaaaaataa ggagggtaat
    11041 atgaagataa cagatatcaa atttgaaaag cttagaatta aattaaaaaa acctgtagta
    11101 gtttcatttg gagtaattga atatggagaa agtataattt taaaaattga aactgatgaa
    11161 gggtattgtg gatttggcga agcagcgcct cttgcagcag ttacaggtga agttttggat
    11221 aatgttcttt cagtattgct tatgtttaaa aaagagttaa taggcaaaga cccactagat
    11281 attgaaacca tacatacaat catggatgga attattatag gtaatacttc tgcaaaagca
    11341 gctattgaca tagctttata tgatattaaa ggaaaaatta tgaatgcacc cctttataaa
    11401 gttcttggag gatttgatag taaagtccaa acggatatta ctataagtat agataagcca
    11461 gaaaaaatgg cagtagaagc tttagaaaga gtaaaagaag gttttaggat attgaagctt
    11521 aaagcaggta taaatcctga agacgatatt gaagctgtaa agttaattag agaagccgtt
    11581 ggagagagta taagaattag aattgatgca aatcaaggat ggaacgtaaa tagcagtata
    11641 aatactatca aaaagttaga agaatttaat gttgatgcaa ttgaacaagc acttccacat
    11701 tgggatttag atggtactgc ttacataaga aataaaagta atacaaaaat tatgatagat
    11761 gaatcattac attcaccaat agatgcaatt aaggctataa aaaagaatgc agttgataca
    11821 tttaatatta agctgatgaa atcaagtgga atatatcctg ctataaaaat caacaacata
    11881 gctgaggcaa gtggagtaaa ttgtatgtta ggatgtatgc tggaaacgag aattggtata
    11941 acagcagcag caaatttaat agcatctaaa aagaacataa cagaagctga ccttgatagt
    12001 tttatgtttt gtgaagaatt agaaggtata tctggaggat ttgtaatgga tggagatatt
    12061 atgaatcttg tcaataaacc aggtttagga atagaagtaa acttatgata aatttggagg
    12121 ttaaatatga atacactttt ttcaaatcca ggtagtatac ttgctgtaat gacatcaatg
    12181 attgcattgg gtttttactt acaaagatac aaagccatta aaagcttagg accagcactt
    12241 acaataatca taatgggaat aatactttca aacttaaaag tagttccagt aagtactgag
    12301 ttatatggaa ctatttcaac atatgctatt ccagtttcta tgactataat gttgatgagt
    12361 gttgacttaa aagaaatgac aaaattatca agagaacctt taattgcaat ttttgtagct
    12421 gtattaactg taagtatcat ggcattttta tttggattag tatttgcaga aaaaatatca
    12481 gaaggttgga aggttgctgg aatgtttgta ggtacatata ctggtggaag tgctaatctt
    12541 acagctattg gaacaggtct taatgtaagt agacaaactc ttgctgcagc aaatgcagca
    12601 gactatgtaa ttggagtacc aacattgata tttatgtttg ctctaccagc aatattaaaa
    12661 aattcaaaga agtttaaaaa actttggcca tatcatgtag aagaatcaga attagaggat
    12721 tgtcaaaatg aagaatttat ggagtcaaaa gaatggagta ttaaagatat agcttggatg
    12781 ttggctatag gttttgttgt tacagaagtt gcaacaatac ttgctggata tttcaattcg
    12841 agttttagta gtgcagcaag aatactttta gttacaacaa tttcaattat aattgctcaa
    12901 ttaaagccag taaagaaact aaagggaaat ttagatttag gattatttgt tgcattattc
    12961 tttttatgta caataggttt ttcagtagat ataaaagaat ttttaggttc aacttttact
    13021 ataacacttt actgttttag cataattttt gcttcatttg ttttccattt aggcataact
    13081 agacttttaa agataaagta tcaatatgta atactttcta tagtaggagc aattgctgat
    13141 ggtcctactt cagctttagt agctgcttct gcaaagtgga attcgcttgt aagtgtagca
    13201 gtcgtaatgg gagtaattgg tggagtatta ggaaattatg caggtatatc agtggcatat
    13261 gcaataaaga tgtctctagg attatagggg ggagtttaat tgataaaatt taaattatac
    13321 atatctggac tatacagtgg aaatgtaatc tttgatggag atttattaat tgaaaagttg
    13381 aatcctttta caaataaaat agaaagttta aaaccaataa gcaaggaaga aaatacatac
    13441 tatttaaatc ttacaaaaat agatttaaag tctttattta ataattttga tgtatatact
    13501 aaaagcttag taaacacaga taaagacaca gttataaata acttaggaga aacgcattct
    13561 aaacttaatg aatatatatg ggttcaaaga aacaaaaagt tcccactaga tataattatt
    13621 gtagataata aaatagtagg ttttatctgt ttatccagag aaacttgtac tatattaatt
    13681 atggatggat atgaagagta tactgtatta aaagaatggg aaaaaactca taaaaatgaa
    13741 gagatatatt ctataagatt tggtggaaat tatatgattg atatgaaaga tggtataaaa
    13801 ctttctacag atgtatatct ccctgatttt gtagattcta ccaaaaaagc accgacaatt
    13861 cttatgagaa ctccatatgg aaaagaaaat gataaagaaa tatattataa atatgtacaa
    13921 agaggatatg ctgttgtaat acaagatgtt aggggaagaa atgaatcaga aggtaagtgg
    13981 gaacctatga ttcatgagag ggaagatgga gatagtacca taaattggat agtatcacag
    14041 gaatggtcaa gtggaatagt tggaatgctt ggagcatcat atcttggtta tgtccaatgg
    14101 gcagctgcat cttcaggcaa taagcatctt aaagctttag ttagtatagt aacatcagga
    14161 agccctttta ttgatatacc aagaaaagga ggagcttttg tatctggtat gcttgcatgg
    14221 gcatttatgg tatcaagaaa taaagttgat agaagcaaaa tggtaagaga tgattgggat
    14281 gatgttttaa acatacgacc aatagaaaat atacctgttg aagctcttgg atatagaatt
    14341 gaatttctgg aagaatggtt aaagagagta gaaaaagatg agtattggga tttgatggat
    14401 tggcatttac aaaaagataa aataaatgta cctgcattgg ttgtatctgg gtggtatgat
    14461 gataatagta tgggaactac agaggcctta gatgttataa aggactatga aaaaggaaag
    14521 agaaaggcta ttctaggtcc atggatgcat aattcaaata ctttaagaga tataaatggt
    14581 atttctttag gaaatagttc attaagatat gatttggatt ataattatct attatggttt
    14641 gataaatact taaaaggtat agaaaataac atagatacga ctgctccagt agagtattac
    14701 tctgtaggtt ctaataagtg gaagacagaa gaaaattggc caataataaa taagatagat
    14761 aaaagtatgt atctaataag tgatggaaat gctaacacat ctttaggaaa tggtagatta
    14821 gtatttgata atggtttaga agaaaagtat gatagttata tatataatcc aaaagaccca
    14881 tcagtacaac taattgatat gtcagagaat gaagttggag ttccaaataa ctacaaagat
    14941 ttagaaaaaa gaagtgatat gttgtgttat acatcagatg cttttagtga agaatttact
    15001 gtaacaggtg atataaagtt agagtttttt gcatcaagtt ctgccaaaga tactgactgg
    15061 gttataaaaa ttatggacgt ggatttagat ggaaattcaa ttaaattagc tgatggtata
    15121 cttagtgcta gatttagaaa tagtttttat aagtcagagt ttatggaaga aggagagatt
    15181 tataagttta cgattataac ttcaaaaata tcaaacacgt ttaaagttgg tcataaaatt
    15241 agactagata taacgtctag tgctaaaaac tttattttcc aaaatagcaa cacgacagag
    15301 ggatataata gtattgaata tatagaagca aagaatacga tttatcatgg aggtaaatat
    15361 ccatctaaat tgatattgcc aatagagaat aagtaaattt atatagatta aatagtgagg
    15421 aggtgtctca aaatgaattt tttagttcgt gagacacttt cttttataca aaatcaaatg
    15481 tattttcatc atttcaacaa tgaaaaagag gcttatctct ctgttttgag atagcctcct
    15541 ataagctaaa ttgattattt tgtagctgca acaacgctca tttcaactaa caaatgctct
    15601 ctagcaagtc tagcttcaac acaagctcta gcaggttcaa atcctttttc aacccaagca
    15661 tcccaaacag agttcatagc ttcaaagtct ttcatatctc ttaaataaat agtaacagat
    15721 agcaaatgtt gcttatctga accatatttg tttaataaat cttctatttt agctaaaact
    15781 tcagttgttt gttcaattac tcctccttca gcatgagttt gtccacataa ataaactgta
    15841 ttgttatgaa ctacagctct actcattctt ccagttcctt catatctctt tatttccata
    15901 ttttaaatac ctccatagtt aaaaaatcta ttaaaaatct aaataataaa ttaaaaaaag
    15961 tctatactat ataaaattat attatatatt ctgataataa tccataaaaa catataactt
    16021 tttagctata attattattg ttattaattg aaaagattta ttagttactt gacatagttt
    16081 acacttttaa ttatagtata aattggaata tttagataat tattatttaa tgtataagct
    16141 aagtttttaa ttaatatggt tttatacatt tatatagaaa agaggtacgt atgagtagca
    16201 caatcaaatc aaatcgaata aaaggtataa tgtttataat agcatcagcg cttggctttg
    16261 caatgatgtc tgcattcgta aagctagctg gagatttacc aagttttcaa aaagtgttct
    16321 ttagaaatct ggtatcagct ataatagctt tttggcttat cataaaacat aaaggaagtc
    16381 taactggtaa aaaagaaaat ttaaaagtac ttttatatag gtcaatattt ggaactctag
    16441 gagttatatt taactactat gcaattgaca agttgatgct ttcagatgct aatatgctaa
    16501 ataaaatgag tcccttcctt gtagtaatat tttgtgcaat tgctctaaaa gaaaaaataa
    16561 atgtaaagca gataggagct attataattg catttatagg agctttattc ataataaaac
    16621 caacatttag tgtagaaata attccatatc ttgggggaat tgcaggagct gtatttgcag
    16681 caatggcata tacttgttta agagttcttg gagatagaga agattactat acaattgtat
    16741 ttttcttttc agttttttca ttagttactg tagggccaat tgcttttgca gtatatgaac
    16801 caatgaccct tatgcaactt atatatctat tactaggggg agtatttgca agtctaggtc
    16861 aatttggaat aactctagca tataaatatg cacctgctaa ggaaatctct atatttgact
    16921 atagtaatat aatcttttca gcaattttaa gtatattctt attcaatgtc tatccagata
    16981 tgttaagtgt tgttggatat ttaatagtat tttcggcagc attttatatg tttttataca
    17041 ataagaagtt agataaatta gacaaagaaa agaagaagta aactagataa aagtgttttt
    17101 ataatttgta ttgaatattt aagtctttgt attaaaatta aagaaaattt aattaaaaat
    17161 gtttggtata ttaacttata acagcagtta aaattgaaaa attataactg ctgtttcttt
    17221 tattaaaaat atccaaatgt ctgtaaaaat agactataat agagtagtat aaataatata
    17281 aaggggataa attaaagtat ggaaaaagtg aaagttgctg tgattggagc aggtaataga
    17341 ggaacgtatg catatgctcc atatatttat gaaaattctg atgtatgtga aatagttgct
    17401 gtagcagaac ctaaaaaagg aagacgagaa ctatttacac aaaaatataa cctagatagt
    17461 aaaaatgtat ttgaaacttt agaagacttt tttaaacatg acaagatggc agatgcagta
    17521 ataatagcta caaatgatga tagacattat gatgtagcaa aactagctct tgaaaaaggt
    17581 tatcatgtac tattagaaaa gccaatgtct aatagtttag atggtctggt acatatagat
    17641 gacttgtgtg ataagtataa agataagatt tttatgattt gccatgtact gagatattct
    17701 ccattttata ataaattaaa agaaattgta gaaagcaaaa aattaggaga gctagttagt
    17761 atacaatata atgaaaatat aggatattgg cattttgcac atagctttac tagagggaat
    17821 tggagaaata gcaatgaaac cagcccttta attttagcaa agagttgtca tgacatggat
    17881 attttgttat atttagtagg aagtagatgc aaaaaaatat ctgcatttgg aagcttaaag
    17941 catcttaata accaaaatgc tagtggagag atggcacaaa attgtcttca gtgtttagtt
    18001 gaaaaaaaat gtccatattc agcaaagaga atttatttag aaaaagatag aagtataaat
    18061 agagctgttc acataaatcc aactgaagag aatctattaa atatacttaa gacaagtcca
    18121 tatggaagat gtgtttacag atgtgataat aatgttgtag acaatatggt aaatatacta
    18181 cagtttgaaa atggagtgac ggctactttt aatttatgtg catttactaa agaaaatgga
    18241 agaactataa agttaatgtt tagccatggt gaagttggtg gggatttaaa taaaaatgag
    18301 ataagaatta aagaatttgg aaaaaacgaa gaaatagtta tgaatccatc aaatcaacag
    18361 aatatggtag aggaatatga tagaaatctt atagctgaat ttattaaatt agtttcaaat
    18421 aaagaactag aaaagggtag agtagcagca aaagaagcta tacaaagtca tgtaatggca
    18481 tttgcagcag aatattctag ggtcagtgat gaagtagtgt atatagaaga attttttgat
    18541 agtgctaagc aaatgactaa ggagatagaa gaaactatat tttaattaat aaaaatttat
    18601 taaataaaat aaagatagat taaataatag aaatacgaaa atatgtgttc tattaaggaa
    18661 tctatctttt ttattaaggt tttttcttat ccctatcaaa aaaatccata cattctgata
    18721 aaagataaga aagtaaagcg aataagattt ctgtacttaa aggaatcgtt actgaatcca
    18781 taaaggtatc aattatgttc atagctagaa aagttagtat aaaatcaatt ataaattcgc
    18841 aaagtttata tattgagtat ggtaatttgt ttacatatct gaatatatct aaaatagtag
    18901 tgcatagaaa atcaattgga gtggttatac aaaaatatat taaaaagaaa attaatacag
    18961 ctccaagaga tttatattca aatcctaaga atgataagaa gccaattcct ataaccgtga
    19021 aaatacaaaa agttatacca aaaaaaacag caaaaaataa aaatacagaa ataaattcgc
    19081 ctaaaggaat ccttctttta ttacttatct taatcaactc cttaatttat gatatgaaat
    19141 ttatataaat aataacattc ttaaaaattc ataatagtcc tctgtttcaa atagtactgt
    19201 ttgagaatct atttttttca tatttggata aaaagaggcc ttaaaagctt tataatgttc
    19261 tctaaatttt atttctatct taaatttatc tggcaatttt attaagtgcc tagataaatc
    19321 tccagataaa gcttttttga cacctttttc aatttttttt agtgcaaggt ttggatggat
    19381 tgaaattgaa ccatttccaa ttccttttga cacaggaaca gtgactatat ttggatttag
    19441 tttttttgca ctctcacaaa gcatttcatc tccacttaaa aatgctacag gaacattata
    19501 atatgcagca atataagcat ttatagtaaa ttcagatgct atttcatcat ttattttaaa
    19561 atagtcataa tcaatatcca tagtatggga aagtggatta ccattacaag tagctccaga
    19621 gtgatagccg gtgaatatac atgcatcaaa gctttcatca attccagcca tcataactag
    19681 tgggtctttg gtccatcctc taagtatttt gacgttttca ggtaaaatat ttggattaat
    19741 atttctacca ctgtcatgtg catctttaac aagtatttca tgtacaccag attgagtagc
    19801 tccatcacat gctgctgtta tctctttact catttgtgtt ctatagtgtt gcgaaaattc
    19861 acattcaagt tttgtttcat cccaatttac aataccacaa gtaccttcta tatcagcact
    19921 taaatacaat ttcataatat ataccccctt atgtttatta ttgatgataa taacagataa
    19981 cagaaaatac ataatcaata aagggcataa aaatattatg tcttactatt aattattatt
    20041 aaatctgtaa ctgaaaaata actaaattgg tattaaaaaa tataacacta tgtgtcattt
    20101 atttatatag tttttatatc tgatgtttat ataaaattgt aacatataag tggttacatt
    20161 tactagttta acatagttaa aatagttagg tattattttt aagttaaatt gattattata
    20221 aaatcgcaga ataaaagaaa gatttaattt attaaaatgc atgaaatgtt ttttcctcaa
    20281 cataatgcta aattaaactc aaaaagaaat aatagaaaaa tcttatgtag cttagaaaga
    20341 atgtgaatga tttaagggaa aaatagaaat ttattatata atattaggct gtattatata
    20401 atacagccta atattatatg tatatgtgac ataaaatctt ggtaagtgta aataaaataa
    20461 atgtggaaaa caagttaatg aaatcaagaa aaaagtaatt taaaattcaa tttcatcttc
    20521 ccaattaaat tctcctgatt tttctacact cttaggccag tgactacacc atttaggaat
    20581 ggacggattt tcaaccctat caacgcttgg agtatatggc tttaagtcta taacaggact
    20641 attatcatta gcatcaatat atggtatttt tattatacca ttttcatggt cgatattgat
    20701 aatttgtaca gcagtcaatg ctattggatt gggtcttata ggtgaccttg ttgcaaatat
    20761 tcctataacg tctggtccac ttttataagg ttttggggtt tctaaaatac ttcttagttc
    20821 aggattatca aacccatcag cccaccaaaa tacatttaaa tgactgaaat cgtcgagttc
    20881 atttaatgca ggtacatact ttttgtctaa tttaataaag acctcttcgc cttgaataca
    20941 tattttacct ataggattta ttaatatttc tttcataata ttacctccaa ttatttaatt
    21001 tatcatttgt aattttaggg tataacatga catcatgtga gggtcaatat aaaataataa
    21061 ataaaaatat taatagttag aatgataata cattttttac atggagcatg acaccagtaa
    21121 taaggcaatc ttcgtcaata tcaaaagtag aggtatgcaa aggggaagta atgttttttt
    21181 cacgatttgc acaaccaagg tggtaaaaag ctccttcaca gtgtcttaga aaatacgaaa
    21241 agtcttcagc cccaagagtg ggcgagtctt taaggataat attttcttct ccaaacaatt
    21301 ctacagcatt ttgtttgacg taatctacta gatggtttga attgataaga gctggataac
    21361 cttcctcaat ttcaacagaa atacttcctc caaatgtttt acaagtaaaa tcgcaaattt
    21421 ctctaattcg tttttttgaa aactctctag tttcaggagt caaggttctc aaagtacccc
    21481 ttataaccac tttatcacaa actatatttt ccttgattcc accttcaatc tttcctagag
    21541 aaataaccac agaatcagtt ggatttgtgt ttcgactaac gattgtctgt agagctgtaa
    21601 taatatgagc agatataaca atagcatcaa taccttgatg tggataagct ccatgacatt
    21661 ttgaaccatg tacagttaat tgaagtgtat cagtggaagc atttagagtg ttgtatttta
    21721 attcaattag atttttgttg atattggaat ttacatgaag tccaaatata tattttacat
    21781 ttgggttttc aagacatcca tcttctatca taagttgagc cccaccaata gtttcctcgg
    21841 caggttgaaa aaagaatttt acatttacat ttaagtaatc ctttaattta aatagtatat
    21901 tacaagtacc cagtaaaata gcagtgtgtg catcatgacc acatgcatgc attttgccaa
    21961 tattaataga tttataagaa taattaagtt cttctgttat aggtaaggca tctatatcag
    22021 ctcttatagc aactgtttca aagtttggac tcacgttaat gtatgcagtt atccctgtat
    22081 gattcttata ttcaatataa tttataccta tttcatttaa gtatttaata atttttttct
    22141 tagtttgaaa ttcttctaat ccaagttctg gagtcttatg taaatctctt cttatattta
    22201 taacccaatc atttatctca tgacaaagag tttttatatt aagcataaaa cacatcctaa
    22261 ctatattaaa aatttatttt tcttcattaa ttatattatt ttatgtaaat cattattatg
    22321 tatcaagtac aaataaaata tcttataatt atattagaga tatttagtat ggagaagaat
    22381 atatcatttt ataagaagag gtgtttaaat gttaggaata attgcgccat ctgggccatt
    22441 aagaaataca agtttggagg aaataaagtt taatttagaa agttatggtt atgaagttaa
    22501 gttttctgaa agttgtagtt taaattataa aggttattta gctggtaatg atgatattag
    22561 agcaagggat atagaagata tgtttttaga taaagatgta gatataatta tgtgcttaag
    22621 aggaggttat ggaacaacaa gaatattgga taaaataaat tatgatataa taaagcaaaa
    22681 tccaaaacca tttataggtt tttctgatat aacaggttta aatttagctt tctataaaaa
    22741 ttgtggtctt ttaccatatc atggaataat ggcagcaaat gtaggtaaat gggataaatt
    22801 tacctataaa tcattggtaa atgctttaga atttaaagat gaattatatt tagaaaatcc
    22861 aaaagaagaa aagatttata ctgtatgtga aggtaaagca gagggaataa taatgggagg
    22921 aaatttatcc ttaataattg ccacaatggg aacaaaatat gaaatagatg caaaagataa
    22981 aattttgttt attgaggaaa ttggagaacc tcaatataaa ctggatagaa tgttaacaca
    23041 actatattct tcaggaaaac ttgaggaatg taatggtata atttttggag attttaagga
    23101 ttgtattgaa gaaaatgatt tgatggaatt actaatagaa tttgcaaata aggtcaataa
    23161 accttctata tataatcttc agtcaggaca ttgtatccca atgataacta ttcctttggg
    23221 cagaatgtgc gaattagatg ctacagaaaa aatcgttaaa ttaaaaaaat agtgtgtata
    23281 taaatataat agaaatatgg tataatctta tacaagtaaa aatttagcta ggaggatgaa
    23341 aaatgtctga tgatagaata atagacttta acgaactaaa gaacaaagtt aaagattctg
    23401 atgtagataa atttgagcaa tacatatata acttatattt ttctgttatg gatggaaaga
    23461 tgtctatggc tgagttttca agaaaaatat tcgattatat gagagataac aacatatctc
    23521 aagaaaaatt tatgaaaata caaaagcaat ttatggagag atatggtatg gacactgaag
    23581 aagtagaaaa acaacttaga aactttggaa tcgacccttc tacggcaggt tttatgagta
    23641 ataatacttc cagcaaagta tctacagaag atttagagtc atttaagaag agtgcaggat
    23701 tttatgagaa atatggagaa aaaatccaac caaagagttg tataacaaca tttataaaaa
    23761 atgacttaaa tgatattaat gtaataatcg accaagaaaa gataatgctt tgtagtgata
    23821 gaaaaataaa tcttatggat tcagaactaa atgagttttt attagaatat aagaatatgt
    23881 ttaataaaaa aataaaggta gtaatgtgcg aaactacgaa taagtatgat tattaaaaaa
    23941 tgaatttaaa tactgaagta gtatcaatta attgtactac ttaaaaataa aaatcgcaat
    24001 ttattattcc ttataaattt gtataaggaa tatctaaact gcgatttttt atattgtgaa
    24061 gattagtaat aatagatagt gttttaaatt gtaatcttct cactaaaact gtgtttttct
    24121 attagaaatt ttattaaaaa taaatatctg ataattgtat tatatgggat gtaaagtatt
    24181 agaagcttaa cgaatctctt ttaatacatt aactccattt tgaatcatac tgttgaaaaa
    24241 tactaagttt aaaatctcag cagggtgaac gttaggaata tcataagtat ctactaaatt
    24301 cataggtaca actatatttt tttcaatatt atgttgatta aaatatgatt ttagagttat
    24361 tgcaaattga tatatacata tatctgtgca atcgccaact ataattatat tatctatatt
    24421 atcaaaatcc aaattttcta atgcaaaaaa accattagta gaatttttag gaagaatttg
    24481 taggttttct atcgacttaa gttcatcaac caattcactt tctacatcat tttccaaaca
    24541 gtgaacagga taactcaaaa gttctattga tttaggagta tgagaatcag taaatgctat
    24601 tactctattt aatttgtttg atattttttt tgtaaacatt tcaattggct ttattaaaga
    24661 ctctactcta ggagagtata atgcaccttg tcttgcaaag ccattattaa catcaattat
    24721 aaataaagca gttttactca aatcataatt ttctattttt tctattggta gattatctaa
    24781 attactcttt aaagcttcaa gttcatttaa taagttatcc atagttatac ctccaagata
    24841 aatttaataa aattgtacat attaattata taaaagtcaa ggagtacaaa ctattgtata
    24901 attagctgta ttgttttgag tgtaaaagat ttgaagcaat atctggatag ttagtaaaaa
    24961 taccatctac attgagtttt agacagtgta gtatatcatc ctcttcattt acagtatata
    25021 cattgacacc aagtagattt ttgtgacaaa gttcaattag agattcattt actaaaacta
    25081 gaggtggatg aagataacta ttacccagaa atttagcaaa attgactaca ttgtttatag
    25141 gtaaataaca caataatcct gtttttacac tagggtctaa tttatgaaag ttttttaagc
    25201 tattgctgtt gaaactagat ataagaactc tatttttcat attatacttt aaaataagct
    25261 ctaaaacgcc tttttctata ttgggatagt ttattttgtc agtttttagt tcgatattta
    25321 aagtaaggtc tgtaggtgag attagaatta atacttcttc taaagttgga attttacatt
    25381 ctttattgct ttgatatcct tcaaaagatg agtttagagt ttttaatttt cttaaagtaa
    25441 agtcttttac aaaaccatgt ccattaaaag ttctatcaac cttttcatca tgaataacta
    25501 caatttttcc atccttagtt ctgtggacat caagttctat tccatatata tccatatcca
    25561 aacatttttt aaaagctaga atagtatttt ctggatactt tccactaaac cccctatgag
    25621 cataaatatt catttatatg gttccctcct ataagtataa cttatcttat aaaaatttaa
    25681 ttaatataat atatcatatt
//
LOCUS       DAMJVK010000051        25641 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.052, whole genome shotgun sequence.
ACCESSION   DAMJVK010000051 DAMJVK010000000
VERSION     DAMJVK010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25641
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.052"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(<1..1003)
                     /locus_tag="PC414_002200"
     CDS             complement(<1..1003)
                     /locus_tag="PC414_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HDF2464595.1"
                     /translation="MKRVYIDFEMNMPNSKSKRAVFNSDIIAIGAVVYDEKTKNIDKF
                     KSLIRPVSNEELYPHIQELTHISSEELKSAPSYEEVMRKFKKWLGIFSDIKGIYTFGN
                     LDLTCFNNTDMRSAKKNNHPRFVNNIRDLFVDIKEKYINCGMRCMNYISLKNLLEFVN
                     LEFSGDAHDPLNDAYNLFILDEAITNNVDIQNLLIIRDIIRPPFNDINSNLDNCFNKF
                     KESLYKKEGNYNIVDFSVEIIKTVRMYLLTIIDVNIQNLEIMKDISKKMDTIDKLKDI
                     EEGYFYLLEDVYFDMKDVLEDLMLYRMNEDEYKYEIKNIIKMFDEDLDNEKIYINKCN
                     "
     gene            complement(1051..1911)
                     /locus_tag="PC414_002201"
     CDS             complement(1051..1911)
                     /locus_tag="PC414_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HDF2464596.1"
                     /translation="MSELSGKGCEIIVPFEERLPVRDIEKSIIKKYRKNLWSKFMKAI
                     RDYKLVEEGDKIAVAISGGKDSILMAKMFQELKKHGQVNFDVEFIAMDPGYHANIRQL
                     LIDNCEYLNIPIHLFDSRIFEIADEIAKDYPCYMCARMRRGALYSKAEELGCNKLALG
                     HHYDDVIETTMLNLLCAGNFKTMLPKLNSTNFEGIKIIRPLYYIREEHIIRFIQNSGI
                     WPLNCACMVAAKKTGNKRYEIKDLIKSLESNFKNVEKSIFKAAENVNLDSVLGWQKDG
                     EKHSFLENFE"
     gene            2180..2389
                     /locus_tag="PC414_002202"
     CDS             2180..2389
                     /locus_tag="PC414_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HDF2464597.1"
                     /translation="MENKTRDIKKNCGPSLARPYINNQIFTEMYCLSDALKFGTIFPE
                     LNLLESEMYNKELYAKPKKYRGGRR"
     gene            2389..2655
                     /locus_tag="PC414_002203"
     CDS             2389..2655
                     /locus_tag="PC414_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HDF2464598.1"
                     /translation="MKDSSRAELMRKVQETSFACVDMNLYLDNHPDDKNAINTYNSLC
                     NQFAQARYAYENKYGPLTNFGYAPSRCPWQWADQPWPWDREFNY"
     gene            2684..3256
                     /locus_tag="PC414_002204"
     CDS             2684..3256
                     /locus_tag="PC414_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000265278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HDF2464599.1"
                     /translation="MWIYQKTIQHPVNIKTCDPRMAKFLITQFGGPNGELAASLRYLS
                     QRYTMPTGNMRALLTDIGTEELAHVELICTMVYQLTSDASPEELKAAGLGSNYAQNGY
                     GIYPTDSNGVPFDVRPIAVMSNPVTDLHEDMAAEQKALATYYQLINLTDDVDVIDVLK
                     FLGQREIIHYQRFGEALMDAYELEESQKMF"
     gene            complement(3491..4681)
                     /locus_tag="PC414_002205"
     CDS             complement(3491..4681)
                     /locus_tag="PC414_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDF2464600.1"
                     /translation="MNFNQKRIAASIMATAIIMPTMGNLAYANESEVESVSIESRTIT
                     GNAVNFRKGPGTNHESMGKLYKGDKVEYVGKEGSWVKVKYNGNTGYVHGNYVAINSLG
                     SSNESSDTSVKSTKVVTAKGLNFRTGPSTSSSKISTLGYGTEVGYISESNGWSKISSN
                     GRVGYVSSKYLGTSVNDSTNENAENSSNDLVKGTKVVTAKSLNVRTGPGTSHSKIATL
                     SYGTEVGSISESGGWTKVSYGNQTGYVSSQYLAEKGSVDTSIPSYSTNSPSQGADSVI
                     SFAKTLLGKPYVWGAEGPNSFDCSGFTQYVMKKSVGVSIPRVSRDQSKYGTYVNRGDL
                     RSGDLVFFDTQGSNNGSVSHVGIYIGNGDMIHASSGSSKKVTISNINSSYYSSRYVNA
                     RRVL"
     gene            complement(4941..5372)
                     /gene="dut"
                     /locus_tag="PC414_002206"
     CDS             complement(4941..5372)
                     /gene="dut"
                     /locus_tag="PC414_002206"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HDF2464601.1"
                     /translation="MYNLKVKKLNDDAIIPNFAHKGDAGMDLYSIEEVVIPPGETKLI
                     KTGICIELPTMTEAQVRPRSGLALKHSVTVLNTPGTIDEGYRGELKIILINHGKNDFK
                     VEKHMKIAQMIVKPIYDINIEEVKELSDSERGKGGFGSTGF"
     gene            5485..7713
                     /locus_tag="PC414_002207"
     CDS             5485..7713
                     /locus_tag="PC414_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HDF2464602.1"
                     /translation="MKLVLAEKPSVAKTIASFLGAKTRQDGYFEGNDYIITYAVGHLV
                     SLYDMKDYDKDKYSGSWKMNNFPFVPEDKFKFKIDSSKTKQFNTVKKLLNRKDVEYVI
                     NATDNDREGELIAFLIFLLAKNKKPVKRILVNEWTPEDITRGIKNLKDEDEMRNLQAA
                     GYTRLITDWLIGINFTSVATLKYGNGKLLNIGRVILPTVKLVYDRDMEILNFVPKTYY
                     EIEGHFKAEAGEYKGKYVKGKESKFDTLEDANKIIASITSETGKILDKKVTMSKEYAP
                     KLFSLTSLQGYITSKYSNFTSDKVLNVCQSLYEGNGKGGYITYPRTDSIFLEESLASK
                     ASQTLDKLKVGLEYENKIKFSKTKRVFDSSKVDSHSAIIPTYIIPKSLSKDEQLVYDA
                     IKDRFVANFMPPAEYENTEIKTEVDNCTFLTKGKVLKSKGYLEVYNKEEKNDLLPLVN
                     KDDVVDVLEIKPLTKQTTPPKPYTEDTLLKAMKNCGKNVPEEDTTVLSGYSIGTSATR
                     ADVLKKISQVGYVKKKGKSYSITDLGKNLVEIFPVKDLFDVDYTGKLEKSLSDIQKGK
                     YTRKEYLTNIISFIYQNVNIIKQDASKNINTEAYTFDSKTKKFTKTKEKKQKKDVTNT
                     EKEVAISKDSNNKKEVKNGDENQSLGKCPICQGDVLEFDKGFACKNHKECKFVIWKND
                     KYLSLYKKKVNKTMVKNILKKGETKVKSLTAKNGNKFDATLKYDKNPDTGYFNWKIEF
                     DN"
     gene            complement(7824..8309)
                     /locus_tag="PC414_002208"
     CDS             complement(7824..8309)
                     /locus_tag="PC414_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2464603.1"
                     /translation="MDNNNILKIADSFLDFLFVLENNIFKESDLLKKFQNNSDIMKAY
                     FEECPMAPSHAKVILYLMTSNSSSISQIASNLGILKSNMTPIIDRLVEHGLVNKFPDP
                     KDRRILRVELTDKAFKLFDAVKAILKESLVKKLSNLSEEELTLLDEHTLKLSEIVKKL
                     G"
     gene            8561..9784
                     /locus_tag="PC414_002209"
     CDS             8561..9784
                     /locus_tag="PC414_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HDF2464604.1"
                     /translation="MKRYLIVFLMCSSLFLVGCGKTEESEPEKPIAVSVQKAIGGEIE
                     NTNSFSGTTKVKDETAVTAQTVGTVQEVYVKLGQNVRKGDELLSISSPELENSVKQSK
                     ASLDLAKASYSSATGGSLEAQVNQAKTALDNAKIQYEESQRNYDNNKILYEQEVISLD
                     QFKKIEFSLEQTKQQLDSAQRAYDTATSKSIPQAKALAKKQLDQAQVSYNLAMSNLDK
                     LTLTSPVDGTITAKNFDSKEMITQSQPAFIISNPSILEVDLNVAESDIGKFKKDGNVD
                     VIIEDQRILGKIDYVPSVVDPQTSLYPVKVLVNNTNNKFKAGMSAQVNLSVEKENGAV
                     TVPKKAIFEENGKKYVYIATKDNIAKKHLVQTGIVTEDKIEIKSGVSDKDTVIIGGIS
                     LISDGTKIFPVEKEK"
     gene            9788..12922
                     /locus_tag="PC414_002210"
     CDS             9788..12922
                     /locus_tag="PC414_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HDF2464605.1"
                     /translation="MNLTQTSVKRPLTIIMVFLVLIVFGGIGYKKMSINLMPDIEIPV
                     VMVMTTWTGAGPQDVDEQVSQKVDESLSAVSNVKSTISSSQESVSMVVAQFEFGTNLD
                     EIMNDVRSKVDALQTSLPDDAAKPTVLKLDMNAQAIGQLVISGGNENSSQALRKYAED
                     VIQPKIESIDGVTSADLKGGKKAQVNVIADPAVLSNYGVSLSTIKGVLSSSNKTFPYG
                     SITQGEDKIVLRAIDKLESLDDIKQIQIPVKGGNTVRLDNLCSVDYGYADKDSIYRYN
                     GKDSLVIDISKQQDANTVKVMQSAYKYVDELNKENPQFKIKIANDTSSYISESINSVM
                     SNLLISAVIAFIVIFAFLKSVRASLVVAVAIPISIIGAIAILYFTGESLNLITASSLV
                     ISVGMVVDNATVVIENIFKYRKSGRLSLDDCAIEGTRTVTNAILASTLTTIAIFLPIM
                     FTEGIAKITFGALGKTLIASLAFSFIGAITLVPSVFAKLSRGKNSQKMQEKDSPIFDK
                     VSEAYKKLLSVCLRHQRLVVISSLLIFVASLFGMGFIGMDFMPAADKGELSISIELPK
                     GLSLESNDYYVSMTEKKVSDIPEIKTLITTLTSDSNNSKASIAIELESQKNRKRTTDE
                     IEKEIIDRVATVPDCKINVSQNSSVMGGAGGADFTLEMKGPDLDVLKILGKEAEKEFS
                     SIKGFRNVETSLTDTTQEAQFTIDKHKAQEYGVNTAEIAGMLRTAINGDSVTTATIDD
                     YKVDVNLKFKADTIDSLEDIKQLKVNSATGKEVPLGAFSDIKMADGLQSISRTDGDFS
                     VNITATLDNLDAGTATKLALEKTNKMVIPKDYSIGVGGETEMMNESMASLGFSMVIAI
                     ALVYMVMVAQFESFSKPFIIMFCVPFAFVGVVLSLLVSGQSLSTVGMLGIILLIGIVV
                     NNGIVLIDYIEQLRKTNRNNDLIGLVSIGCAARLRPVLMTTATTILGMLPTALSLGEG
                     GETMQPLAIVIMGGLTVSTLITLVLIPTIYLIFDKWENKFSDKMPKIFGKITDKIGSI
                     KRKIIKKPKLKAKV"
     gene            12956..14254
                     /locus_tag="PC414_002211"
     CDS             12956..14254
                     /locus_tag="PC414_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HDF2464606.1"
                     /translation="MSKIVAIGCGVGIITANLIPVYADNNTSSINKVDNSILSDSNKR
                     TSEKEILTAREAVSAALKNSEKLKMKSEEIKMLKEKLEVQDEFDSFTGSDNSFPYDQI
                     GLLKNQSEQAKGFMEDQIANDITNKFNDLVSRENELDKIKNNLEIKTKEIKDMKLKKD
                     LGLVTSLETESAELELQTLQNTQKAKLQELKNNQDYFKLLTNIDLNNYQLDKEYRFES
                     FRVGGSVDSYMEGKVNEYLKYDQLILERTEESFNDKDENKADLPDRPNLTRPVAPTKP
                     EQGNLTDDEYKVLTDKYEKDYKRYEKEVEAYNLERQTYAAGLTTYANYLQQKFNTENG
                     LVTLEDSKKALKKGLIDSYAQLLALEDTIQITKKQLDLSEKQLKNTKLRYDLGLITLT
                     DYKKQVVSNEDAKNSYDTLIVNYNSLKNGIEKPWILNTGK"
     gene            complement(14543..14788)
                     /locus_tag="PC414_002212"
     CDS             complement(14543..14788)
                     /locus_tag="PC414_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF2464607.1"
                     /translation="MTKCPKCGKEVSSRPGSICPRCGFKVADESLKIRCPEPSCRALV
                     SRKLDYCPKCGCKLRGYNISEFVNMVRCKIDETFDKK"
     gene            complement(15002..17629)
                     /locus_tag="PC414_002213"
     CDS             complement(15002..17629)
                     /locus_tag="PC414_002213"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HDF2464608.1"
                     /translation="METKYVYSFGEGSKDMKSLLGGKGANLAEMTKIGLPVPPGFTIT
                     TEACNDYYVNNESIRAEIIKEIEEHLATLEKDLNKTLGCNKNPLLVSVRSGAVFSMPG
                     MMDTILNLGLNDNSVVGLAEATQNERFAYDSYRRFIQMFSDVAMEVPKYKFENVLDRV
                     KEAKGYTVDTELTTDDLKEIVKEFKAIYKKEIKNDFPQDPKEQLMLAIEAVFRSWNNP
                     RAIVYRKLNDIAHNLGTAVNIQSMVFGNMGETSGTGVAFTRNPATGENKLFGEFLMNA
                     QGEDVVAGIRTPQNISTLADVMPAVFDEFVKITHILEGHYKDMQDIEFTIENERLYIL
                     QTRNGKRTAAAAINVAVDLVEAGIIDEKEAIMRIEPNQLDQLLHPKFEDKALKEAKVI
                     AKGLPASPGAASGKVYFNADDVVKANEKGEKVVLVRLETSPEDIEGMVKAEGILTARG
                     GMTSHAAVVARGMGKCCVAGCGEIKVDEFNKEIRALDDVVIKEGEYISIDGSTGNVYL
                     GDVKKTEVSLTGNFEKLMNWVDKHKCMMVRTNADNPRDARAAIEFGAEGIGLCRTEHM
                     FFDEARLPAVREMILSNTVEQREKALEKILPMQREDFVELFKVMDGKPVNIRLLDPPL
                     HEFLPHDDETIEELSKSMGIKVSDIKKRIVDLDEFNPMLGHRGCRLAITYPEICVMQS
                     KAIIQGAIEAIKAGVKVSPEIMVPLVGEVNELKIIRKMIVETVDAIIKEEGIEVPYTV
                     GTMIEIPRACLTADEIAQEADFFSFGTNDLTQMAFGYSRDDAGKFLGQYVDEEILEKD
                     PFQVLDQNGVGKLVKMGAKLGREVKPELKLGICGEHGGEPSSVEFCYSVGLNYVSCSP
                     FRVPIARLAAAQASIKNPR"
     gene            complement(17690..18532)
                     /locus_tag="PC414_002214"
     CDS             complement(17690..18532)
                     /locus_tag="PC414_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HDF2464609.1"
                     /translation="MLVKNLIIYAVSDSVGETAQQVAKACMSQFYVNETYEIKRFPYM
                     INKGVLLETLENAKAENALIVYTLVDEELCSIVERYCEREGLSCIDLMTDILREISKR
                     TGRKPKREAGIIRKLDESYFKRVEAIEFAVKYDDGKDPRGVLQADIILVGISRTSKTP
                     LSMYLANKNIKVANVPLVPEIPIPKEVFEIDTKKIIGLTNSPEKLNEIRTQRLKALGL
                     SSKANYANLERILQELDYSEEIMKRIGCPVINVSNKAIEETAGIILDIMKENGLKIYK
                     EIEI"
     gene            complement(18539..19177)
                     /locus_tag="PC414_002215"
     CDS             complement(18539..19177)
                     /locus_tag="PC414_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2464610.1"
                     /translation="MIIIQLNERQLKIIDIVKENEPITSESIASSLNVTRATLRSDLA
                     ILTMTGILDARPKVGYFYSGVSEINLIGKSVKEKTVEDIMSMPVLAKKDESIYDVIVT
                     MFLSDVGSIVIIDENEELCGVVSRKDLLKATIGGSDINKMPIGMIMTRTPNVVTLTKG
                     ASVLLASRKIIEHEVDSIPIVEYKGEDKNHMRVVGRISKTNITKLFLEIVDN"
     gene            complement(19613..20905)
                     /locus_tag="PC414_002216"
     CDS             complement(19613..20905)
                     /locus_tag="PC414_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDF2464611.1"
                     /translation="MLDLNRKLAKLEEEGKKIKVALIGAGHMGNGMVSQMANMKGIEA
                     SIVVDIDLELAHKAFTDAGIDEEIIDNVTNEKDAEIKLQEGKVLTCNDFLVACKTKSI
                     DVVIDATGGIAIGAEIALNSILNKKHIVMLNAETDCVVGPILKKLADDAGVIFTGSAG
                     DEPGAVMELFDFADAMGFEVRVIGKGKNNKLDLDCNPDTVREEAERKGASPHMIASFK
                     EGTKTMVEMALMCNATGFVPDVRGGHGIEATVNEVPKKYALKSEGGVLDNYGVVDFVN
                     GIAPGVFVVVAHKLKAVNDELKYLSMGDGPNYILYRPYHLCSLETPLSAAMAVLDHKA
                     TIVPKAGLVAEVMTIAKKDLKKGEFMDGYGAYTCYGTIEKYDVAKAMNAVPIGLISKK
                     TKVVKDIKKGEVITYDMVEIEKDTTLYHLRQLQEKIFG"
     gene            complement(20942..21298)
                     /locus_tag="PC414_002217"
     CDS             complement(20942..21298)
                     /locus_tag="PC414_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDF2464612.1"
                     /translation="MKYEVKISGIGSLVKELMDESNCLIIYDETINDDDLKDISVIHS
                     IATLKSDVEIGDTLTIGNRDYCIVSVGDIAQKTLREIGHCTIKFDGKCEVNLPGEIHV
                     EIGNPDITIGDLITIS"
     gene            complement(21333..21692)
                     /locus_tag="PC414_002218"
     CDS             complement(21333..21692)
                     /locus_tag="PC414_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464613.1"
                     /translation="MKLLVILFVAYILNTIFIMKQNKLYFKTLNRAKKKGDIVSTGKK
                     KSYFSKGSIAVISSDSDGFIKFGEILKGRTVMAKFKEIEEIEGLDIYSAEQKFKDEES
                     IVQAIKFIKEKINVSFN"
     gene            complement(21761..23734)
                     /locus_tag="PC414_002219"
     CDS             complement(21761..23734)
                     /locus_tag="PC414_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDF2464614.1"
                     /translation="MSVTEKIPSIDRKIFKILIMCSKKEFVSINSIANELNVTTRSVR
                     TYIKQLNKDLGNDIAVIKYIKGQGYKLEIKDEQILNKIIDMNRKNVFSLNSKEDRVEF
                     ILNYLIELDGFITLDSLADEMCVGRTTLVNDFQYVEKVLASYNLNLIKKQNTGMKLNG
                     NELDIRLFILNQLYKNSRKDFSNSKYFKGIKKEEIINLEEKLLTLFKKNNFYVTDEML
                     REVINYIVVLVYRVKEEKKVKDYDVKFDLLKSYDEYFIAREIKHIISGMFECTLNDEE
                     IIYLTIPLVSGNAPASECALNSSRISKNIDELMENIFNQIYVDMGIFINEDELRVGLG
                     YHLSFTLNRLLFNIKLKNVLLEEIKQNYILPFKLAQIAGKVIEKKYNLEVSEDEIGYI
                     AIHFSGYLERNSSRFYSIKKIAIICSTGLGTAKLLKIRVEKLIGNNPKIDTLSSFNLR
                     NINLDEYDIVFTTIDLDTSNINTIVLKINTIFDENKLREQLKTVLCLREGNIDTTNST
                     NLLINNLLDEDKFMILNEKTILASLEKMMDHLMDLGCIDEKFRKNIVNREEKSPTVFD
                     KGLLFPHSINEKSDKFLMAVGILEEPIEYANRSIKIILMTMFPCENKIDSDLLVKIYE
                     EVLKIGQDVKLTNKISKCRTFLEFKKVLLKNLI"
     gene            complement(23956..24969)
                     /locus_tag="PC414_002220"
     CDS             complement(23956..24969)
                     /locus_tag="PC414_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDF2464615.1"
                     /translation="MEENKILKIEKGTSGWGGPLYIKKEGNRNKILSMTAGGIHEVTL
                     KIKELLGCEIVDGFKTGVSDEEVAVVIIDCGGTARCGVYPKKKIPTINVNPVGKTGPL
                     AKFITEEYYVSDVNPNCISVVDGEDMPQKSQENKSENKSSIRKPDNYDEVKSKAQGEY
                     AKKNIILSIGQGAGQVVSKFYDAGRDTIQMVMNNVIPFMAFVSMLMGIILASGLGDWI
                     ARVISPLAGNIGGLLIISVICTLPFLSPILGPGAVIAQVVGTLVGTQIGLGAIPAYLA
                     LPALFAINGQAGCDFVPVGLSLGEAEPETVEYGVPALFYSRLITGPIAVIIAYGVAVF
                     ALR"
     gene            complement(24982..25527)
                     /locus_tag="PC414_002221"
     CDS             complement(24982..25527)
                     /locus_tag="PC414_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDF2464616.1"
                     /translation="MENVITGLSKGAEWFIGLFQKGGEQFIGLVSGTLPTLIVLMVAI
                     NSLIKIIGEERVNNWASKLGKNSFTRYILLPLVSVFFLGNPMCYTFGRFLKEEHKAGF
                     YDAAVSFVHPITGLFPHANAGELFVWLGISAGLTTLGKETTTLALWYFIVGLIVIFIR
                     GIVTEKMYAFLTRKNRATNKA"
BASE COUNT         8769 a         4271 c         3266 g         9335 t
ORIGIN      
        1 tattacattt atttatatat attttttcat tatctaagtc ttcatcaaac atttttatta
       61 tattttttat ctcatacttg tactcatctt cattcattct ataaagcatt aaatcttcta
      121 gtacatcttt catgtcgaag tacacatctt ctaataaata aaaatagcct tcctctatat
      181 ctttcaattt atctatagta tccatttttt tacttatatc tttcataatt tctaaatttt
      241 gtatatttac atctatgatt gttaataagt acattctcac agtttttatt atttcaacag
      301 aaaaatctac aatattataa tttccttctt ttttatataa tgactcctta aatttgttaa
      361 aacaattatc taaattagaa tttatatcgt taaatggtgg tcttattatg tcacgtatta
      421 ttaaaagatt ttgtatatct acattatttg tgattgcctc atccaaaata aataaattat
      481 atgcatcatt taatgggtca tgggcatctc cactaaactc taagttaaca aattcaagta
      541 aatttttcaa tgatatatag ttcatacatc tcataccaca atttatatat ttttctttta
      601 tatctacaaa taaatctcta atgttattta caaaccttgg atggttattt tttttagcac
      661 ttctcatatc tgtgttattg aaacatgtca aatccaaatt accaaaagta tatattcctt
      721 taatatcaga aaatatgcca agccattttt taaactttct catgacctct tcataacttg
      781 gtgcactctt caattcttca gaagatatat gtgttaattc ttgtatatga ggatacaact
      841 cctcattact tactggtctt attaaagatt taaatttatc aatatttttt gttttttcat
      901 catacacaac tgctccaatt gcaattatat cagaattaaa tactgctctt tttgatttag
      961 aatttggcat attcatttca aaatctatat atactctttt caatataaac accctttcca
     1021 attgtttata gtgattcttt ttaactattt ttattcaaaa ttttctaaga aagaatgttt
     1081 ctccccatct ttttgccaac caagtactga atcaagattt acattttctg ccgccttaaa
     1141 tatagatttt tctacatttt taaaatttga ttctaaactc tttattaagt cttttatctc
     1201 atatctttta ttacctgttt ttttagcagc aaccatacat gcacaattta atggccatat
     1261 accactattt tgaataaatc ttataatatg ttcttctctt atataataaa gtggtcttat
     1321 aatttttata ccttcaaaat tagtagaatt aagtttagga agcattgttt taaaatttcc
     1381 agcacacaat aagttaagca ttgttgtctc tattacatcg tcataatgat gacctaatgc
     1441 aagtttgttg caccctagtt cttctgcctt tgaatataat gctcctctcc tcattcttgc
     1501 acacatatag catggataat cttttgctat ttcatctgct atctcaaata tccttgaatc
     1561 aaataaatgt attggtatat ttagatactc acaattatct ataagtaatt gtcttatatt
     1621 tgcatgatat ccagggtcca tagcaataaa ttcaacatca aaattaactt gtccatgttt
     1681 ttttaactct tgaaacattt tagccataag aatactgtct tttccaccag aaatcgctac
     1741 agctatctta tcaccttctt caaccaattt ataatctctt attgccttca taaacttaga
     1801 ccataaattt tttctatatt tttttattat acttttttct atatctctta ctggaagtct
     1861 ttcctcaaaa ggaactataa tttcacagcc ttttcctgat agttcactca tctaatcacc
     1921 tcgtatattt atgttaataa tattttacaa aaaagtctac cttcatccac aaaatactat
     1981 aatttttact ttaattttac taaataattt atagtttaca ataacacata aacttataat
     2041 aacataaata acctctttct tttattatct tttttatttt tctcatttaa tattttatta
     2101 ttagtaacga tttttaaatg attgaatatt atacactaga tagagttacc tatctattta
     2161 caacaaggag gttttcatct tggaaaataa gactagagat atcaaaaaaa attgtggtcc
     2221 atctttggct agaccataca ttaataatca aattttcaca gaaatgtact gcctttctga
     2281 tgcattaaaa tttggaacta tatttccaga actaaactta ctagagtctg aaatgtacaa
     2341 caaagaatta tatgctaagc caaaaaaata tagaggaggt agaagataat gaaagactca
     2401 tctagagctg aacttatgag aaaagttcag gaaacatctt ttgcatgtgt tgatatgaac
     2461 ttatatttag acaatcaccc tgatgataaa aatgctatca acacttataa ttcattatgt
     2521 aatcaatttg cgcaagctag atatgcttac gaaaataaat acggtccttt aactaacttt
     2581 gggtatgctc caagtagatg cccttggcaa tgggctgacc aaccttggcc ttgggacaga
     2641 gaatttaatt attagacatc aagtattgga ggattgaaaa ttcatgtgga tatatcagaa
     2701 aactatacaa cacccagtta atataaaaac ttgtgaccct agaatggcta aatttcttat
     2761 aactcaattt ggtgggccaa atggagaact tgctgcatct ttaagatatt taagccaaag
     2821 atatacaatg cctactggta atatgcgtgc acttttaaca gatattggta cagaagaact
     2881 agctcacgtt gagcttatat gtactatggt ttatcagtta acttctgatg caagcccaga
     2941 agagttaaaa gctgcaggtc ttggttcaaa ctatgctcaa aatggatatg gaatttatcc
     3001 aacagattca aatggtgttc catttgatgt aagacctata gcagttatgt caaatcccgt
     3061 aaccgattta catgaggata tggcagctga acaaaaagca cttgcaactt attatcaact
     3121 tataaaccta acagatgacg ttgatgttat agatgtatta aaattcttgg gtcaaagaga
     3181 aataattcac tatcaaagat ttggtgaagc tttaatggat gcttacgagt tagaagaatc
     3241 tcaaaaaatg ttctaaaatt tttatttgta taactattat tatttgataa tttataattt
     3301 actgttgtgt taaaatttat aaaaaatata aatataattt attttctata aacagagaaa
     3361 ataaataaaa agcaggtatc aatcaaatag taacatgtat tatactatac attactatca
     3421 atattgtacc tgctttcata ttataagcaa taaagaaaaa tctatttatt aggttttata
     3481 ataaaaacaa ttataaaact cttcttgcat ttacatatct acttgaatag tagctactat
     3541 ttatattaga tattgttact ttttttgatg aacctgaaga tgcatgtatc atatctccat
     3601 ttcctatgta tattccaaca tgacttacag aaccattatt tgaaccttgt gtatcaaaga
     3661 atactaaatc tccacttcta aggtctcctc tatttacata tgttccatac ttactttggt
     3721 ctctagatac tcttggtata ctaactccta cagatttttt cataacgtac tgtgtgaatc
     3781 cagaacagtc aaaactattt ggaccttctg ctccccatac atatggtttt ccaagtagtg
     3841 tctttgcaaa acttattaca ctatcagctc cttgacttgg agaattagtc gaataagatg
     3901 gaattgaagt atcaacagaa cctttttcag caaggtattg acttgacaca tatccagttt
     3961 gattgccata acttactttt gtccatcctc cactttcaga aatacttcca acctcagtac
     4021 cataacttaa tgtagctatt ttagaatgac ttgtacctgg tcctgttcta acgttcaatg
     4081 actttgctgt tacaaccttt gtacccttta caaggtcatt tgaactgttt tcagcatttt
     4141 catttgtaga gtcatttaca ctagttccta aatatttact tgaaacataa cctactctac
     4201 cattagaact tatttttgac catccatttg actcagatat gtatccgact tcagtcccat
     4261 atcctagtgt tgatatttta gaactacttg tacttggtcc tgttctaaaa tttaatcctt
     4321 ttgctgttac aacttttgtc gacttaactg aagtatcact actttcattg ctacttccaa
     4381 ggctatttat tgctacataa tttccatgca cataacctgt gtttccatta tatttaactt
     4441 ttacccaaga accttctttt cctacgtact caactttatc acctttgtaa agtttcccca
     4501 ttgattcatg gtttgtacct ggtccttttc taaaatttac cgcattccct gttattgttc
     4561 tgctttctat actcacactc tctacttcag actcattagc atatgctaaa ttccccatag
     4621 taggcattat tatcgcagtt gccattatac tggctgctat tctcttttga ttaaaattca
     4681 taactttcca ctcatctcct cttttctact atctttattt cacttcaaaa tataaaagaa
     4741 ataattgaca ctattaaaat atcaattatt tcttttatat taatattatt acaaatttgt
     4801 aactatttgt ctatagtaat atttgcttac cataaattac catgtataaa ttttaatctt
     4861 taatagcctg taatttagta atatagccat cttttacctt ttttaaaaat ggctatattt
     4921 tttaactttt ttgtaacttt ttaaaatcca gttgagccaa acccaccttt tcctctttcg
     4981 gagtcactta attcttttac ttcttctatg ttaatatcat agataggttt tactatcatt
     5041 tgagcaattt tcatatgctt ttcaacctta aaatcatttt taccatgatt tattaatata
     5101 attttaagct ctcctctgta tccttcgtca atagttcctg gtgtatttaa tacagtaaca
     5161 gaatgcttta aggcaagccc acttctaggt cttacctgcg cttctgtcat agtaggaagc
     5221 tctatacata tcccagtttt gataagttta gtctctccag gaggtattac aacctcttct
     5281 atagaataca aatccatacc agcatcacct ttgtgagcaa aattaggtat tatagcatca
     5341 tcatttaatt tttttacttt taaattgtac atcttatatc tcctttcaac tttctataaa
     5401 tattataact taaatttcga cctcttgaag ttttttattt ttttgtggta atataattat
     5461 tagtgtttta aaggaggatt ttttatgaaa cttgtacttg ctgagaaacc atctgtggca
     5521 aaaactatag catcattttt aggtgcaaag accagacaag atggttactt tgaaggaaat
     5581 gattatataa ttacttatgc agttggacac ttagtatcat tatatgatat gaaggattat
     5641 gataaagata aatactctgg ctcttggaaa atgaacaatt tcccatttgt tcctgaggat
     5701 aagtttaaat ttaagattga tagttctaaa actaaacaat tcaacactgt aaaaaagctt
     5761 ttgaatagaa aagatgtaga atatgtaata aatgccactg acaatgaccg agaaggagaa
     5821 cttatagcat ttttaatatt tttactggca aaaaataaaa aaccagtaaa aagaatcttg
     5881 gtaaatgaat ggacaccaga agatattaca agaggtataa aaaacctaaa agatgaagat
     5941 gaaatgagaa atcttcaagc tgctggatat actaggctta ttactgactg gttgattgga
     6001 ataaatttca cttctgttgc tacattgaaa tatggaaatg ggaaactatt aaacataggt
     6061 agggtaattc ttccaacagt taaattagtc tatgatagag atatggaaat actaaacttt
     6121 gttcctaaaa cctattatga aatagaaggg cattttaaag ctgaagctgg tgaatataaa
     6181 ggtaaatatg ttaagggtaa agaaagtaaa tttgatacac ttgaagatgc aaataaaatt
     6241 atagcttcaa ttacatctga aactggtaaa atactagata aaaaagttac tatgtcaaag
     6301 gaatatgcac caaaactttt tagtttgaca tcacttcaag gatatataac ttctaagtat
     6361 tccaatttta catctgataa ggttttaaat gtctgtcaat ctctctatga gggtaatggt
     6421 aagggtggat acataactta ccctagaact gattccattt tcttagaaga aagtttggct
     6481 tcaaaagctt ctcaaacttt agataaatta aaggttggtc ttgaatatga aaataaaatt
     6541 aaattttcta aaactaaaag agtttttgat tcttctaaag tagactccca tagtgctata
     6601 attccaactt atataattcc aaaaagttta tctaaggatg aacaattagt ttatgatgcc
     6661 ataaaagata gatttgtagc aaactttatg cctcctgctg aatatgagaa tacagaaatt
     6721 aagactgaag tagataattg tactttctta acaaaaggta aggtgcttaa atcaaaagga
     6781 taccttgagg tatataataa ggaagaaaaa aacgatttac ttccacttgt aaataaagat
     6841 gatgttgtag atgtgttaga aataaaacca ttaactaagc aaactactcc acctaaacca
     6901 tatactgagg acacactttt aaaggctatg aaaaattgtg gtaaaaatgt gcctgaagaa
     6961 gatacaacag tgctatctgg atattcgatt ggaacatcag ccacacgtgc tgatgtgtta
     7021 aagaagataa gtcaagtggg ctatgtaaag aaaaaaggta aatcttattc tataacagac
     7081 cttgggaaaa atttagttga aatttttcct gttaaagact tatttgatgt agattatact
     7141 ggaaaacttg aaaaaagttt aagcgatatt caaaaaggaa agtatacaag aaaagaatat
     7201 cttactaata tcattagttt tatatatcaa aatgtcaata ttataaaaca agatgcttca
     7261 aaaaatataa acacagaagc atatactttc gattcaaaaa ctaaaaaatt tactaaaaca
     7321 aaagaaaaaa aacagaaaaa agatgtaact aatactgaaa aagaagttgc tatatcaaag
     7381 gattctaata acaaaaaaga agttaaaaat ggtgatgaaa atcaatctct tggcaaatgc
     7441 cccatttgtc aaggtgatgt actagaattt gacaaaggct ttgcttgtaa aaatcataaa
     7501 gaatgtaaat ttgttatttg gaaaaatgat aagtatttaa gtctttataa aaagaaagtt
     7561 aacaaaacaa tggtcaaaaa tattttaaaa aaaggtgaaa ctaaagtaaa atctttaact
     7621 gctaaaaacg gtaataaatt tgatgctaca ttaaaatatg ataaaaatcc agatactggc
     7681 tatttcaatt ggaaaataga gtttgacaac taaatattca taagaaaaga aatagcagta
     7741 gtataataat ctatattata ttactgctat ttcttttctt atgaagtttt aactttttta
     7801 atagtgactt ttacaatcac actctaacct aatttcttta ctatttctga taactttaat
     7861 gtatgttcat ctaataaagt tagttcctct tcagaaagat tagataattt ttttactaga
     7921 gattctttga gaatagcttt tactgcatca aataatttaa atgctttatc tgttaattca
     7981 accctaagaa tccttctatc ttttgggtca ggaaatttat tgactaaacc atgttccact
     8041 aatctgtcaa taatcggagt catatttgat tttaaaattc ctaaattaga agctatttga
     8101 gaaattgatg atgaatttga tgtcataaga taaagaatta ccttagcatg tgatggagcc
     8161 attggacatt cttcaaaata agctttcatt atatcagagt tattttgaaa cttttttaac
     8221 aaatcgcttt ctttaaatat attattttca agcacaaata aaaaatctaa aaaagagtcc
     8281 gctattttaa gtatattgtt gttatccata tttgtaacat cccttcaata gataatataa
     8341 taaaacattg ttgtttaata tagtataaat tataaactat taataaattt aaaacaagaa
     8401 aaagttattg actcataata attataaatt tataatcaaa ttagagaatt ataaattgcc
     8461 aataaaggga agttttttaa ttagtctaaa actaaactta aattataaga cactttaaat
     8521 tctacaacaa gctagtgtgc aatatataat aggaggagat atgaaaagat atttaatagt
     8581 tttcttaatg tgtagttcac tgtttttagt tggatgtgga aaaacagaag aatctgaacc
     8641 tgagaaacca atagcagttt ctgttcaaaa agcaataggt ggagaaattg aaaacacaaa
     8701 ttctttctca ggaactacaa aggttaaaga tgaaacagct gttactgcac agactgttgg
     8761 cacagttcaa gaagtgtatg taaaacttgg gcaaaatgta agaaaaggag atgaattatt
     8821 gagtataagc agtccagagc tagaaaatag tgtaaagcaa agtaaagcct cactagattt
     8881 agcaaaagca agttattcaa gtgccactgg tggttcatta gaggcacaag taaatcaggc
     8941 aaaaacagct cttgacaatg caaagattca atatgaggag tctcaaagaa attatgacaa
     9001 taataagatt ctttatgaac aggaagtcat tagtttagac caatttaaaa aaatagaatt
     9061 ttctttagag caaacaaaac aacagttaga ttctgctcaa agagcatacg atacagcaac
     9121 ttcaaaaagt atacctcaag ctaaagcact tgctaaaaaa cagcttgacc aagctcaagt
     9181 atcatataat ttagcaatga gtaatttaga caaattaaca ttaacatctc cagtagatgg
     9241 tacaataaca gctaaaaact ttgattcaaa ggaaatgata acacaatcac aaccagcttt
     9301 tattatttct aatcctagta tactagaagt tgatttaaat gtagcagaat cagatatagg
     9361 taaatttaag aaagatggaa atgtagatgt tataattgaa gaccaaagga ttttaggaaa
     9421 aatagattat gtaccttcag tagttgaccc tcagacatct ttatatcctg ttaaagtact
     9481 tgtaaataat acaaacaaca aatttaaggc aggtatgtct gctcaagtaa acttaagtgt
     9541 tgaaaaagaa aatggtgctg ttacagtacc taaaaaagca atatttgaag aaaatggtaa
     9601 aaaatatgta tacatagcaa caaaagacaa tatagcaaag aaacacttgg ttcaaactgg
     9661 aattgtaaca gaggataaaa tagaaattaa aagtggtgta agtgataaag atacagtgat
     9721 tattggaggg ataagtctta tttctgacgg aactaagata tttcctgtag aaaaggagaa
     9781 gtagactatg aatttaacac agacttccgt taaacgtcct ctcacaatca taatggtctt
     9841 tttagtatta atagtttttg gtggtattgg atataaaaaa atgtcaataa atctaatgcc
     9901 tgatatagaa ataccagttg taatggtcat gactacttgg acaggtgctg gtcctcaaga
     9961 cgttgatgaa caagttagtc aaaaagttga tgaaagttta tctgccgttt caaatgtaaa
    10021 atctactata tcaagttcac aagaaagtgt ttctatggta gttgctcagt ttgaatttgg
    10081 aactaatttg gatgaaataa tgaatgatgt taggtctaaa gttgatgcat tacagaccag
    10141 cctcccagat gatgcagcta aaccaacagt tttaaagctg gatatgaatg cacaggccat
    10201 aggtcaactt gtcatatctg gaggaaatga aaatagttca caagccttaa gaaaatacgc
    10261 tgaagatgta attcagccta aaatagaatc catagatggt gttacttcag cagaccttaa
    10321 aggtggaaaa aaagcacagg ttaatgtaat agctgaccca gctgttcttt caaattatgg
    10381 ggttagttta tccactataa aaggtgtctt atcctctagt aataaaacat tcccttatgg
    10441 ttcaattact caaggtgagg ataaaatagt tttaagagcg attgataaat tagaatccct
    10501 agatgatata aaacagattc aaattcctgt aaaaggagga aatacagtta gattagataa
    10561 tctttgtagt gtagattatg gatatgcaga caaagatagt atttatagat acaatggaaa
    10621 agacagtttg gtaatagata tttctaaaca acaagatgcc aacactgtaa aagttatgca
    10681 gagtgcatac aaatacgtag atgaattgaa taaggaaaat cctcaattta aaattaaaat
    10741 tgctaatgat acaagttcat acataagtga atcaataaac agtgtaatga gcaacttgct
    10801 tataagtgct gttatcgcat ttattgtaat atttgctttc ctaaaaagtg taagagcttc
    10861 actagttgtt gcagttgcaa taccaatttc tataataggt gctatagcaa ttctctactt
    10921 tactggtgaa agtttaaatc ttattactgc aagttcattg gtaatttcag ttggtatggt
    10981 tgttgataat gctacagttg taattgaaaa tatatttaaa tataggaaaa gtggcagatt
    11041 gagtcttgat gattgtgcta ttgagggaac aagaactgta acaaatgcaa tacttgcatc
    11101 aacattgacc acaatagcaa tattcttacc tatcatgttt acagaaggta tagctaaaat
    11161 aacttttggg gcattaggta aaacactaat agcttcacta gcattctcct ttataggtgc
    11221 aattacactc gttcctagtg tatttgcaaa attaagcaga ggaaaaaata gtcaaaagat
    11281 gcaagaaaaa gatagtccta tatttgataa ggtaagtgaa gcatataaaa aattattaag
    11341 tgtatgtttg agacatcaac gcttagttgt aatatcaagt ttacttattt ttgtagcttc
    11401 cttatttgga atgggattta taggtatgga ctttatgcct gcagcagaca agggtgaatt
    11461 atctatttct atagaacttc caaaaggtct tagcctagaa tccaatgatt actatgtatc
    11521 tatgactgag aaaaaagtct cagatattcc agaaattaaa actttaataa caactttgac
    11581 atcagattca aataatagta aagcaagtat agcaattgaa ttagaatctc aaaaaaatag
    11641 aaaaagaact actgatgaaa tagaaaaaga gataattgat agagtggcta ctgtaccaga
    11701 ttgtaaaata aatgtaagtc aaaacagtag tgttatgggt ggagctggag gagcagactt
    11761 cacattagaa atgaaaggtc ctgatttaga tgtattaaaa atactgggaa aagaagctga
    11821 aaaagaattt agctctataa aaggatttag aaatgttgaa acttctctta cagatacaac
    11881 acaagaagct caatttacta tagataaaca taaggctcaa gaatatggtg taaataccgc
    11941 tgaaattgca ggcatgttac gtacagcaat aaatggtgac tccgtaacca ctgctactat
    12001 tgatgattac aaagtggatg taaacctaaa atttaaagca gatactattg atagcttaga
    12061 agatataaag caactaaaag taaactctgc aactggaaaa gaagttcctc ttggtgcttt
    12121 ttcagatata aaaatggcag atggtctaca atctatatct aggactgatg gagatttttc
    12181 tgtaaatata actgctacac ttgacaattt agatgctggt actgctacaa aactagcact
    12241 agaaaaaacg aataaaatgg taattcctaa agattactct ataggtgttg gtggtgaaac
    12301 agaaatgatg aatgaatcta tggcaagtct tggattttca atggtcatag ctatagcatt
    12361 agtttatatg gtaatggtag ctcagtttga atcatttagt aaaccattta taatcatgtt
    12421 ctgtgttcca tttgcattcg ttggagttgt gctttcgctt ctagttagtg gtcaaagttt
    12481 aagtactgtc ggtatgctag gtataatctt acttatcggt attgttgtta ataatggtat
    12541 agttcttatt gactacatag agcaacttag aaaaacaaat cgtaacaatg atttaattgg
    12601 tttggtttca attggttgtg cagcaagatt aagaccagtc ttaatgacta cagcaacaac
    12661 aattttaggt atgttgccaa cagcactatc tcttggtgaa ggtggagaaa ctatgcaacc
    12721 actagccata gttataatgg gtggtctaac tgtatctaca cttataactt tggtgcttat
    12781 acctactata tacttaatat ttgataaatg ggaaaataag ttttcagaca aaatgcctaa
    12841 aatatttggt aagataacag ataaaatagg aagtataaaa cgtaaaataa taaagaaacc
    12901 aaaattgaaa gctaaagttt aaatagaatg gagggtgatt gtattgaata aaaaaatgag
    12961 caaaatagtt gcaattggct gtggtgttgg tataataact gccaatttaa tcccagttta
    13021 cgcagataat aatacttctt ctataaacaa agttgataat tctattttaa gtgactcaaa
    13081 taaaagaaca tctgaaaagg aaattttgac tgctagagaa gctgttagtg ctgcacttaa
    13141 aaatagtgaa aaattaaaaa tgaaatcaga agaaataaaa atgttaaaag aaaagttaga
    13201 agtacaagat gagtttgata gttttactgg aagtgacaac tcgtttccat acgaccaaat
    13261 tggacttctt aaaaatcaaa gtgaacaggc taaaggcttt atggaggacc aaatagcaaa
    13321 tgatattact aataaattta acgatttagt ttcaagagaa aatgagctag acaagataaa
    13381 aaacaatctt gaaataaaaa ctaaagaaat taaagatatg aaattaaaaa aagatttagg
    13441 gttagtaaca tctttagaaa ctgaatcagc tgaacttgaa cttcaaacac tacaaaatac
    13501 tcaaaaagct aaacttcaag aactaaaaaa caatcaagac tactttaagt tgttgactaa
    13561 tatagattta aataattatc aattggataa agaatataga tttgaatctt ttagagtagg
    13621 tggctcagtt gattcatata tggaaggtaa ggttaatgaa tatttaaaat acgaccaatt
    13681 gattttagaa cgtacagaag aaagctttaa tgataaagat gagaacaaag cagatttacc
    13741 agatagacct aatcttacaa gaccagttgc tcctacaaaa ccagagcaag gtaacctaac
    13801 tgatgatgaa tataaggttc ttacggataa gtatgaaaaa gactacaaga gatatgaaaa
    13861 agaagttgaa gcatacaatc ttgaaagaca gacttatgct gctggcctta ctacttatgc
    13921 aaattatctt caacaaaaat ttaataccga aaatggtcta gtaacattag aagacagtaa
    13981 aaaagctctt aaaaaagggt taattgatag ctatgctcaa ttacttgctt tagaagatac
    14041 aattcagatt actaaaaaac aacttgattt atcagaaaaa caattaaaaa atactaaatt
    14101 aagatatgat ttaggattaa ttactctaac tgattataaa aaacaagtgg taagtaatga
    14161 agatgctaaa aatagctatg acactcttat tgtgaattat aactcactaa aaaatggtat
    14221 agaaaaacct tggatactta atactggaaa ataattctgg taaatagaaa gattacttta
    14281 acatacgttt gattaaaaaa tagttatgca cactcgtggc tttagtcatg gaagattcag
    14341 aaggcaaata atagaaatag attagtaaaa atatatttgc ttataaaact gtataatttt
    14401 atagaggcta tctcaaaata gagataagcc tcttttttat tgtttaaatt ataaaaatat
    14461 atttaattcc atacaaaaag agtgccttat gaactaaaaa gttcattttg agacaccctc
    14521 aaaagcttaa ctctaaattt aattattttt tatcaaatgt ctcatctatt ttacatctca
    14581 ccatgttaac aaattcagaa atattatatc ctctaagttt gcatccacat ttaggacaat
    14641 aatccaattt cctagatacc aaagccctac aactaggttc tggacatctt atttttagac
    14701 tctcatctgc tactttaaat ccacatcgtg ggcatatact acctggacgt gatgatactt
    14761 cctttccaca tttaggacac tttgtcaact ttatcccctc cagtaaactc aatttaattg
    14821 atagttgaaa tttctctcta caataatata tgcaatataa ttaaatacgt ttcacttaca
    14881 actatataag ttaaattttt ataataaaaa tcccctatat tcaatttaca aatacttaat
    14941 ttgatacatt gaacaatagg ggattttatt tttatttagt tacatatttt gcagtctatt
    15001 attatctagg attttttata gatgcttgag ctgctgcaag tctagcaatt ggcactctaa
    15061 atggtgaaca agatacatag ttaagtccta cactataaca gaactctact gaagatggtt
    15121 ctcctccatg ctctccacat attcctagtt ttaattctgg tttaacttct ctacctaatt
    15181 tagctcccat cttaactaac ttaccaacac cattttggtc tagtacttgg aatgggtctt
    15241 tttctaatat ttcttcatct acatattgac ctaagaattt accagcatca tctcttgaat
    15301 atccaaatgc catttgagtt aagtcattag taccaaaact gaagaagtct gcttcttgtg
    15361 ctatttcatc agcagttaaa caagctctag gtatttctat catagtacct acagtataag
    15421 gaacttctat accttcttct tttattatag cgtcaacagt ttctactatc atttttctta
    15481 ttatttttaa ctcatttact tctccaacta atggaaccat tatctctgga gaaactttaa
    15541 cacctgcttt gattgcttct atagcacctt gtattatagc tttagattgc attacacata
    15601 tttcaggata agttattgca agtctacaac ctctgtgtcc aagcattggg ttaaactcat
    15661 ctaaatcaac tattctcttc ttaatatcac ttacttttat tcccatagat tttgaaagtt
    15721 cttctatagt ctcatcatca tgaggcaaaa attcatgtag tggtgggtct agtaatctta
    15781 tattaactgg ttttccatcc ataactttaa ataactctac aaaatcttct ctttgcattg
    15841 gtaatatttt ttcaagagct ttttctcttt gttctactgt attagataat atcatttctc
    15901 taactgctgg caatctagct tcatcaaaga acatatgctc agttctacat agacctatac
    15961 cttctgcacc aaactctata gctgcacgag catctctagg attatctgca ttagttctaa
    16021 ccatcataca tttgtgtttg tctacccagt tcattaattt ttcaaaattt ccagttaaag
    16081 atacttctgt tttcttaaca tctcctaggt atacatttcc tgtagaacca tctatagaaa
    16141 tgtattctcc ttcttttata actacatcat caagtgctct tatttcttta ttaaactcat
    16201 caacttttat ttctccacat ccagcaacac aacatttacc catacctcta gcaacaactg
    16261 ctgcatgtga tgtcattcca cctctagctg taaggattcc ttctgcttta accattcctt
    16321 ctatatcttc tggagatgtc tctagtctaa ctaaaactac tttttctcct ttttcatttg
    16381 cttttactac atcatcagca ttgaaataaa ctttaccact tgctgcacct ggtgatgctg
    16441 gtaaaccttt tgctataacc ttagcttctt ttaatgcctt atcttcaaat tttggatgta
    16501 ataattggtc taattgatta ggctcaattc tcatgatagc ttctttttca tcaattattc
    16561 cagcttcaac taaatcaacc gctacattta ttgctgctgc tgctgttctc ttaccatttc
    16621 tagtttgtag tatatataat ctttcatttt ctatagtaaa ctctatatct tgcatatctt
    16681 tataatgacc ttcaagtata tgagtaattt ttacaaattc atcgaataca gctggcatta
    16741 catctgcaag agttgatata ttttgaggag ttcttatacc tgcaacaacg tcttcacctt
    16801 gtgcattcat taaaaattca ccaaataatt tattttctcc tgtagctgga tttctagtaa
    16861 atgcaacacc tgtaccacta gtttctccca tattaccaaa taccattgat tgaatattta
    16921 ctgctgttcc taagttatga gctatatcat tcaatttacg gtatacgatt gcacgaggat
    16981 tattccatga tctaaataca gcctctattg caagcattaa ttgctctttt gggtcttgtg
    17041 ggaaatcatt ttttatttct tttttataaa ttgctttaaa ttcttttact atttctttta
    17101 aatcatcagt tgtaagttct gtatcaactg tgtatccctt tgcttctttt actctgtcta
    17161 atacattttc aaatttatat tttggaactt ccatagcaac atcagaaaac atttgtatga
    17221 atcttctgta actatcatat gcaaatcttt cattttgtgt agcctctgct aagccaacaa
    17281 cactattgtc attaagacct aggttaagaa ttgtatccat cattcctggc atagagaata
    17341 ctgcaccaga acgaactgat actaataatg gattcttatt gcatcctaat gttttattta
    17401 agtctttttc taaagtagct aaatgctctt ctatttcttt tattatctct gctcttatac
    17461 tttcattgtt aacataataa tcattacatg cttctgttgt tatagtgaat ccaggaggga
    17521 caggtaagcc tatcttagtc atttctgcta aattggcacc tttacctcct agtaaagatt
    17581 tcatgtcttt gcttccttca ccaaaactat aaacatactt agtttccatt gttaaatccc
    17641 ccattaatct ttttatttta atttatatca tttttattaa tagacattat taaatttcta
    17701 tttctttgta tatctttaga ccattttctt tcattatgtc taaaattata cctgctgttt
    17761 cttctattgc cttatttgaa acgtttatta ctgggcatcc aattcttttc attatctctt
    17821 ctgaataatc taactcctga agtattcttt ctaaattagc atagtttgct ttactggaca
    17881 atcctaaagc ttttaatctt tgggttctta tctcattcag cttttcaggt gaattagtaa
    17941 gacctattat ctttttagta tctatctcaa acacttcttt aggaattggt atttctggaa
    18001 ctaatggaac attagctact tttatatttt tattagctaa atacatactc aaaggagttt
    18061 tagatgttct tgatattccc actaaaatta tgtctgcttg aagaacacct ctagggtctt
    18121 ttccatcatc atatttcact gcaaattcta tagcttcaac tctcttaaag taagattcat
    18181 ctaattttct aattatacca gcttctcttt taggttttct tcctgttctc ttgcttattt
    18241 ctcttagtat atctgtcatt aaatctatac aactaagacc ttctctttcg caatatcttt
    18301 caacaatact gcataattct tcatcaacta atgtatatac aattaaagcg ttttcagctt
    18361 ttgcattctc caaagtttca agtagtactc ctttattaat catgtatgga aatcttttta
    18421 tttcataagt ttcattcaca taaaactgag acatacatgc ctttgcaact tgttgtgcag
    18481 tctcccctac tgaatctgat actgcatata taattaggtt tttcaccagc atacctcctt
    18541 aattgtcaac tatttctaag aatagcttag ttatgttggt ttttgatatt ctcccaacaa
    18601 ctctcatatg atttttgtct tctcccttat attcaacaat cggaatcgaa tctacttcat
    18661 gttctattat ttttcttgaa gctaataaaa cacttgctcc tttagttaaa gtgactacat
    18721 ttggagttct tgtcattatc atacctattg gcattttatt tatatcagaa cctcctatag
    18781 ttgcttttag caagtctttt ctggaaacaa ctccacataa ctcttcattc tcatctataa
    18841 taactatact ccctacatca gataaaaaca tagtgactat tacatcatat atactttcat
    18901 ccttcttagc tagtacaggc atgctcatta tatcctctac tgttttttct tttacacttt
    18961 taccaattaa atttatttca ctaactcctg aataaaaata tccaactttt ggtcttgcgt
    19021 ctaatattcc tgtcattgtt aatatagcta aatcagatct aagtgtagca cgagtcacat
    19081 tcaagctgga ggcaatactt tcactcgtta ttggttcatt ttccttaact atatctataa
    19141 tttttagttg tctttcatta agctgaataa taatcacctt ctttcattgt gctatacttt
    19201 ttatcttttc gcatcttata tagtatatta tataactata tataccattc tgtaaacaga
    19261 ttttttattg aatacgatat attttttact aagttctctc gcttaaagat atttattata
    19321 tattaatggg ggatatttct ttaattaata gaactatatt agtattacag ttaatattat
    19381 aatacataat tggtcatttt aattcatttt atattaaaat ttttaataat attatttcaa
    19441 tgtgaatata aaattgcaga actaattcaa aataattttt acgactacaa ataacctata
    19501 atatactttt aaagaaagta tcctaacaag aatatataaa aatttaaaag ggttgattta
    19561 aaaataacca tcaacccttt ttactaatat ctatttatat taccatcaaa tcttacccaa
    19621 atattttttc ttgcaattgt cttaaatgat ataaagtagt atctttttct atctctacca
    19681 tatcgtaagt tataacttca ccttttttaa tatcttttac aactttagtc tttttactta
    19741 tcaaaccaat aggtacagca ttcatagcct ttgctacatc gtatttttct atagtaccat
    19801 agcatgtata tgctccatag ccatccataa actcaccttt tttcaaatct ttcttagcta
    19861 tagtcataac ttctgctact agtccagctt taggtactat tgtagcctta tggtctaaaa
    19921 ctgccatagc tgctgataaa ggagtctcta aactacataa atgatatggt ctatataata
    19981 tgtaattagg tccatcaccc atacttaaat attttagttc atcattaact gcttttaatt
    20041 tatgggcaac tactacgaaa actccaggtg ctataccatt tacaaaatca actacaccat
    20101 aattatctaa tactccacct tcgcttttta atgcatattt cttaggtact tcattaactg
    20161 tagcttcaat accatgacca cctcttacat caggcacaaa tccagttgca ttacacatta
    20221 aagccatttc taccattgtt ttagttcctt ctttaaaaga agctatcata tgaggtgaag
    20281 ctccttttct ttctgcttct tctctaactg tatcaggatt acaatctaaa tctaatttat
    20341 tatttttacc tttacctatc actctaactt caaaccccat tgcatctgca aaatcaaata
    20401 attccattac tgcacctggc tcatctcctg cagaacctgt aaatattact ccagcatcat
    20461 ctgctaattt tttaagtatt ggtccaacaa cacaatcagt ttcagcattt aacataacta
    20521 tatgtttttt atttaaaata gagtttaatg ctatctctgc accaattgca atacccccag
    20581 ttgcatctat aactacatct attgacttag ttttacaagc cacaagaaag tcattacatg
    20641 tcaatacttt tccttcttgt aattttattt ctgcatcctt ttcatttgtt acattatcta
    20701 taatttcttc atctattcct gcatcagtaa aagctttatg agccaactcc aaatctatat
    20761 ctacaacaat agaagcttca atacctttca tatttgccat ttggcttacc attccatttc
    20821 ccatatgccc agctcctata agagctactt ttattttttt accttcctct tccaattttg
    20881 caagttttct gtttaaatct aacataccaa ttcctcctgt tataataatt ttattttcaa
    20941 attaagaaat agttattaaa tctccaatag ttatatctgg atttcctatt tccacatgta
    21001 tttctcctgg taaatttact tcacattttc catcaaattt gattgtacaa tgcccaattt
    21061 ctctaagagt cttctgagca atatcaccaa ctgaaactat acaataatct ctattcccta
    21121 tagtaagtgt atctcctatt tcaacatcac tttttaatgt tgctattgaa tgaattacag
    21181 atatatcctt taagtcgtca tcattaatgg tttcatcata tataataaga caattgcttt
    21241 catccattaa ctctttaact aaactgccta tcccacttat cttaacttca tatttcatga
    21301 cattcctcct gataatttat atttatattg agttaattaa aagaaacatt tattttttct
    21361 tttataaatt ttatagcttg tacaatactc tcttcatctt taaatttttg ctctgcacta
    21421 tatatatcta atccttctat ttcttcaatc tctttaaatt tagccattac tgtacgccct
    21481 tttaaaattt ctccaaactt tataaaccca tctgaatctg aagatataac ggctatactt
    21541 cctttagaaa aatagctttt ctttttgcct gtagacacta tatcaccctt ttttttagct
    21601 ctatttaaag ttttaaaata caatttattc tgcttcatta taaatatagt atttaaaata
    21661 taagcaacaa ataaaattac taataatttc atatttatac accttccaaa aatcacatta
    21721 tttttaaatc tgtataacaa ctataaatgc tgtacaatag ttatataagg tttttaagta
    21781 acaccttttt aaattctaaa aaagttctac atttacttat cttattcgtt aattttacat
    21841 cttggcctat ctttaaaact tcttcataaa tttttactaa taagtctgag tctatcttat
    21901 tctcacatgg aaacattgtc ataagaatta tttttatact tctatttgca tattctattg
    21961 gttcttctaa aattccaact gccattaaaa acttatctga tttttcattt atagaatgag
    22021 gaaataataa acctttatca aatactgtgg gagatttttc ttctctattg actatatttt
    22081 ttctaaactt ctcatcaata catcccaaat ccataagatg gtccatcatt ttttctaatg
    22141 aggcaagtat agtcttttca tttaatatca taaatttatc ttcatctaac aaattattta
    22201 ttagtaaatt tgtagaatta gtagtgtcaa tattcccttc tcttaggcac aatactgttt
    22261 ttaattgttc tcttagtttg ttttcatcaa atattgtatt tatttttaat actattgtat
    22321 ttatatttga tgtgtctaaa tcaatagttg taaaaactat atcgtattca tctaagttaa
    22381 tatttctcaa attaaaactt gaaagagtat caattttagg attatttcct atcagttttt
    22441 ctactcttat tttcaatagt tttgctgttc ctagacctgt actacatata attgctattt
    22501 tttttattga atagaatcta ctgctatttc tttctaaata tccactgaaa tggattgcta
    22561 tatatccaat ctcatcttct gaaacttcta agttatactt tttctctatt acttttcctg
    22621 ctatttgtgc taatttaaat ggaagtatat aattttgctt aatctcttct aacaaaacat
    22681 tttttaactt tatattaaat aatagcctgt ttaaagtaaa actaagatga tatcctagac
    22741 ctactcttaa ttcatcctca tttataaaaa tacccatatc gacatatatt tgattaaata
    22801 tattttccat caactcatca atgtttttac taattctact actatttaaa gcacattctg
    22861 atgctggtgc atttccacta actaacggta tagttaaata aataatttct tcatcattta
    22921 aagtacattc aaacattcct gatattatat gttttatttc cctagcaata aagtattcat
    22981 catatgattt aagtaaatca aactttacat cataatcttt tactttcttt tcttctttta
    23041 ctctatagac taaaactact atgtagttaa taacttctct taacatttca tctgtaacat
    23101 aaaaattatt ttttttaaat aaagtcaata gtttttcttc taaatttatg atttcttctt
    23161 ttttgattcc tttaaaatac tttgaattac tgaaatcttt ccttgagttt ttatacaact
    23221 gatttaatat aaacagtcta atatcaagtt catttccatt taatttcata cctgtattct
    23281 gttttttaat caagtttaaa ttatatgaag ccaaaacttt ttctacatac tggaaatcat
    23341 ttactaaagt agttcttcct acacacatct catcagctaa actatcaagt gtaataaatc
    23401 catctagttc tatcaagtaa tttagtataa actctactct atcttctttt gaatttaatg
    23461 aaaatacatt ttttctattc atatcaataa tcttattgag tatctgctca tcttttattt
    23521 ctagtttata gccttgaccc tttatatatt tgattactgc tatatcatta cctaaatctt
    23581 tattcaattg ttttatatac gtccttacac ttctagttgt gacattcaat tcatttgcta
    23641 ttgagttaat actcacaaac tcttttttac tacacataat caatatctta aatatcttgc
    23701 ggtcaataga aggaattttc tcagttacac tcattttgta tctcctcctt gatttcattc
    23761 tagtacaaat ttgaaaccaa attaatacat aaaatttcac tttaataaag gaaaatttag
    23821 tattaatcta actcgataat tacagctaat taatagtata aaagctagtc tttcattaac
    23881 aaaaaatctt gactagctta aatttatatt tacaatttta ttaattaatt ccaagcctta
    23941 tattgacatt aaatattatc tcaatgcaaa tactgcaact ccatatgcaa taataacagc
    24001 tattggacca gttattaaac gagagtaaaa taaagctgga acaccatact ctacagtttc
    24061 tggttctgct tctcccaaac ttaatcctac tggtacaaaa tcacatccag cttgcccatt
    24121 tattgcaaat aaagctggaa gtgctaaata tgctggtatt gcaccaagac caatttgtgt
    24181 acctacaaga gtccctacta cttgagctat aactgcacca ggtcctagta ttggtgataa
    24241 gaatggaagt gtgcaaatta ctgatataat caataatcca ccaatattac ctgcaagtgg
    24301 agatataact cttgcaatcc agtctcccag tccagatgct aaaataattc ccataagcat
    24361 acttacaaat gccataaatg gtattacgtt attcattacc atttggatag tatctcgacc
    24421 tgcatcataa aattttgata ctacttgccc tgccccttga ccaatagaaa gtattatgtt
    24481 ctttttagca tattcacctt gtgcttttga cttaacctca tcataattat caggctttct
    24541 aatagaactc ttattttcgc ttttattttc ttgagatttt tgtggcatat cttccccatc
    24601 tacaacactt atacagttag gattaacatc tgatacataa tattcttctg ttataaattt
    24661 tgctaaagga cctgtttttc caactggatt tacatttatt gtaggtattt ttttctttgg
    24721 ataaactcca catctagcag ttccaccaca atcaataata actactgcta cttcttcatc
    24781 agaaactcca gttttaaatc catctacaat ttcacaaccc agtagttctt ttatttttaa
    24841 tgttacttca tggataccgc cagcagtcat tgatagtatc ttatttctat tcccttcttt
    24901 ttttatatac aaaggacctc cccagcctga agttcccttt tctattttta atattttatt
    24961 ctcctccatt ttatctacct cctaagcctt atttgttgct ctattttttc ttgttagaaa
    25021 agcatacatt ttttctgtta ctatacctct tataaaaatt acaattagac caactataaa
    25081 ataccacaat gccaatgtag tagtttcttt acctaaagta gtcaaacctg cagatattcc
    25141 caaccataca aaaagttctc ctgcatttgc atgtggaaaa agacctgtaa ttggatgtac
    25201 aaatgaaact gctgcatcat aaaatcctgc tttgtgctct tcttttaaaa atctaccaaa
    25261 tgtatagcac ataggatttc ccaagaaaaa tactgatact aatggaagta gaatatatct
    25321 tgtaaaagag ttctttccta gcttagaagc ccaattatta actctttcct caccaattat
    25381 cttaatcaaa gagttaatag ctaccattaa tacaattagc gttggaagag tacctgaaac
    25441 aagacctata aattgttctc cccctttctg aaacaaaccg ataaaccact ctgcaccttt
    25501 acttagacct gtaataacgt tttccataaa attataacct ccctataata tctttaaatt
    25561 tttaatttta ctgcttacca taaattttac aaattacatc ttttttctgt actatatgct
    25621 aatattgtaa atgttttttt a
//
LOCUS       DAMJVK010000052        24796 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.053, whole genome shotgun sequence.
ACCESSION   DAMJVK010000052 DAMJVK010000000
VERSION     DAMJVK010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24796
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.053"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            1..455
                     /locus_tag="PC414_002222"
                     /pseudo
     CDS             1..455
                     /locus_tag="PC414_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899647.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            842..1135
                     /locus_tag="PC414_002223"
     CDS             842..1135
                     /locus_tag="PC414_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464617.1"
                     /translation="MERGNRIICDQTGKILLQTGEATGDILEHDEITELHFVDIPYGS
                     IDYTRNRIIGINIETKEPILEEIPTFISDEEKRIQELENQILLNENEKVGGIL"
     gene            1135..1317
                     /locus_tag="PC414_002224"
     CDS             1135..1317
                     /locus_tag="PC414_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464618.1"
                     /translation="MNINNVVVRILAERILNKGLNPLKNRVFELDDVTNTEYRMAVEN
                     YIIEHSEVVEGAELTK"
     gene            1358..1588
                     /locus_tag="PC414_002225"
     CDS             1358..1588
                     /locus_tag="PC414_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HDF2464619.1"
                     /translation="MNEELVKEKISTLETRVKEHGKQIDRIEIEQAKFAIQIQNLCSD
                     IKNLTGVLKWLVGVIITTLVGFFIFAIQKGIF"
     gene            1608..1865
                     /locus_tag="PC414_002226"
     CDS             1608..1865
                     /locus_tag="PC414_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDF2464620.1"
                     /translation="MDNLISFIPEQLLLLVVALNVLGFGFKKYKQLDNKYIPIILLVI
                     GIGFSIWMLGFNPSSILQGILCWGVAIGANQVYKQLKDGDK"
     gene            1865..2671
                     /locus_tag="PC414_002227"
     CDS             1865..2671
                     /locus_tag="PC414_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2464621.1"
                     /translation="MKIAIVPGHTLSGKGTGATGYIDEGKENRILTDLIVKWLKQGGA
                     IVYTGKVDKSNNYLAEQCKIANRQNVDLAVQIHFNANKTTLNPMGTETIYKTNNGKVY
                     AERVNDKLATVFENRGAKSDVRGLYWLKHTKAPAILIEVCFVDSKADTDYYIKNKNTV
                     AKLIAEGILNKNINNKENGEGKIMYKHTIVYDGEVDKIPATVVGWGYNDGKILICDIK
                     DYVPGQTQNLYVVGGGACEKISSMTKENYTMIKGNDRFDTLHKVLDFIDR"
     gene            complement(2717..2929)
                     /locus_tag="PC414_002228"
     CDS             complement(2717..2929)
                     /locus_tag="PC414_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464622.1"
                     /translation="MTWKNEIKSILVKENISMNELNNLMNEHNNKNNTVENLRQKINN
                     ETMKYSEILTIANLIGYEVVWKKKEK"
     gene            3108..3941
                     /locus_tag="PC414_002229"
     CDS             3108..3941
                     /locus_tag="PC414_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464623.1"
                     /translation="MKILFKEAHKMAREIKRKYSNVDYKVQFSLCLSFLNKKGDIKLK
                     ELKGSEKQIIWANDIRTDILNLTNELEKSKIERIKNEDYKIKDMSIEEMTERCKRKFE
                     RIREAISNIENAKFFIDNFRNVLKYNSLNQKAFHINQILKDSQFAKENRNWSFLQGET
                     QASYRLKKVKDYSISYEEAKKIAKEINFNDNWSNEIKENVLEIVEIFDDAVKQATSTE
                     YIEKAKKYIIETLIQKNAQFYRNGFADIKKKQIENYRKIESDERKIEIAYQVFRDGVE
                     C"
     gene            4018..4521
                     /locus_tag="PC414_002230"
     CDS             4018..4521
                     /locus_tag="PC414_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464624.1"
                     /translation="MNIDFSTILDLKVNKENVKMIEKEINKINNDIFDISLKDCANGH
                     IGSVKETVGKSLNIETWGHGEINSKIGIRLRFNFYYSKGDKVDIENIENILIYVNSLC
                     SKNVESDLIANVRPKFYKLEEKYSTRIEKCEIRHEFIGINKQEIKEFLEYLTEIEKSE
                     RTVIYKK"
     gene            4553..5023
                     /locus_tag="PC414_002231"
     CDS             4553..5023
                     /locus_tag="PC414_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464625.1"
                     /translation="MDKKEAKKFLKVIDMNIDKIEEEAIKAFKESCLIKETNDIKIYI
                     DIQGKVEAIAIQAYAKLLDDDKEINIFTSRQTGNNLDDMFGEIYYVNDYEEFENWCEN
                     KCESLNWDSYKKFNKENFEEIAERNIDDSTSVFLEELREGIESCKQELQNIVEN"
     gene            complement(5332..5637)
                     /locus_tag="PC414_002232"
     CDS             complement(5332..5637)
                     /locus_tag="PC414_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464626.1"
                     /translation="MNVEQSKSKKVYLNIGEELSKKMTKSELNKFSQELANYLQYAGL
                     DKNMKIKLFQTILEMVARKKITLSSDSEFTEGILQSDEAEMTTNVGFIIEGLSYHND"
     gene            complement(5847..6047)
                     /locus_tag="PC414_002233"
     CDS             complement(5847..6047)
                     /locus_tag="PC414_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464627.1"
                     /translation="MCFKTYVLKRVVNNNQLEITRSFDLDNLICTATEILKDNEIDLI
                     IIDEKNNVVWKNEKSTPQQENA"
     gene            complement(6180..6329)
                     /locus_tag="PC414_002234"
     CDS             complement(6180..6329)
                     /locus_tag="PC414_002234"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464628.1"
                     /translation="MFKNKMDKCTHVLTAYISSSYDYYNFLDTQLDDFILEYGENVVS
                     LVYTK"
     regulatory      complement(6383..6480)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            6689..7078
                     /locus_tag="PC414_002235"
     CDS             6689..7078
                     /locus_tag="PC414_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2464629.1"
                     /translation="MKQQEVVNKLRRGELMLMEYLWRKNSILSKKEIIEAMKEKYKWR
                     KSTTEILLKRLVKMKTLKKKRIGFQFSYEVLVTKKEYLNAIKEDRKVTKYDSFFIRMF
                     TTMHKKEKMTEEQIKKYIENMEKIGKE"
     gene            7172..7555
                     /locus_tag="PC414_002236"
     CDS             7172..7555
                     /locus_tag="PC414_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2464630.1"
                     /translation="MGFKKLPDSELKVMKFIWGLDGNKVASGEVVEAMKQIYDWNKNT
                     TLTILSKLAKKNYLYSQKTSKCTYYTIAVREKDYLKVETKKFFSFFHNNSLKSFFTAL
                     NDEENLSDDKLDMLEEWVKNWEEDE"
     gene            8234..9664
                     /locus_tag="PC414_002237"
     CDS             8234..9664
                     /locus_tag="PC414_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF2464631.1"
                     /translation="MILNQIFIEAGGIMRNKIIIGITFLLVLLMSGCASKQTAEINVL
                     ATTDLHGVIPYELTSYVKEERKKDKNLTLVDAGDFFDSGEVFGSSMDSYFSARYKDGD
                     NNTESYIETPISKDMKEVGYDVAVLGNHEFISNNKFYLDNMISDFEKQKINILSANTY
                     KKNGDSYTKPYIIKNINTPEGNVKLGILGLTIKEVGERKQWKDGKLVDSKSLELKDQE
                     GYNGELYMNDLVEDAKKWVSIMEKEKPDIIVAVAHTGEKPKKPKNPGNRIQDLAQQVD
                     GIDAIVAGHNHVQIEQHDYKNKSGENVIVTEPGKHGECISKINFKLEKNNDKWNIVDK
                     SSKLIKFEKNQESDKFGEFISSINKVSKDKKEIRLSEVKTPEWDKIYVFKSGTSADDI
                     YKTIGYKWRNISSPKSDGMVQIVVMNNNKPVSYIYGEAEVMPINIKCDESEYKDNVVT
                     IYPNKNDKFKIEKGKQEYSINLKHIQ"
     gene            complement(9812..10162)
                     /locus_tag="PC414_002238"
                     /pseudo
     CDS             complement(9812..10162)
                     /locus_tag="PC414_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904812.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            10157..10678
                     /locus_tag="PC414_002239"
     CDS             10157..10678
                     /locus_tag="PC414_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HDF2464632.1"
                     /translation="MFSIGGGYAIIPLIQEKVVNQTGWISEKIFTDIITISQMTPGPL
                     AVNTSTFVGLQIGGFWGAVAATLGCVLCGVVISLFLYNFFQKHQNSTYIFEILNGLKS
                     ASLGLIISAAATIIILALFGTNKVYFNSIFEMLNWTALIIFFVTLLIVRKWKINPIVI
                     IIMSGILGIFFYL"
     gene            complement(10807..11043)
                     /locus_tag="PC414_002240"
     CDS             complement(10807..11043)
                     /locus_tag="PC414_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464633.1"
                     /translation="MLECFIAKDSKIKDGVNIPTLVFKTLHDRYFKTQYFDSYYNSLK
                     CKKKLVEINNVHNSYYIDSKKFCQEAYDWFVENQ"
     gene            complement(11353..14781)
                     /gene="uvrA"
                     /locus_tag="PC414_002241"
     CDS             complement(11353..14781)
                     /gene="uvrA"
                     /locus_tag="PC414_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDF2464634.1"
                     /translation="MKINNYKNQSIITNPKKFENKYQDLPKTPIELLKVVQSLVIHGD
                     QGKLYGISFNKQQSDEELLRTIPQMLKRIFEINSNPLTIPRNPKQRLVGMCRDYSLLL
                     VSLLRYRGFEARMRAGFANYFESELTYEDHWLVEYYDTLTKRWIRIDAQIDDIQKNYF
                     QINFDTHDVGKTDGFLTGSEAWIRCRQGHAHPDDFGYNKNWKGWHSVKGNLLHDFNNM
                     IGLELLPWDLWTELSSKKYNQLTRAEKNLLDEMAEILSSGNIKIEALNLLIEKLPEDY
                     LKSIFSQLKILGISEIKELGNPLELEKKFKFTKAINKSIKNSLCHNKSSIYLKGGRQN
                     NLKDVEVTIPKNQITVITGVSGSGKSSLAFDTIYEEGKRRYFENLSNGAKLSEQLQKP
                     EFDLLQGLTPTIAIEQKKGSQNPRSTVGTLTSIWDYLRMLFVSIGKSYCPYCKIPLEK
                     KNNTKNYCPHCQTIFSKINTSTFNANSHTGACHDCNGLGFTYQVNPQLIVKDPTISIL
                     DGATYYFGKLRGKKPNGNWMVGELYAIAKDKNIDLDIPWNELPRDFIDAILYGTDDKI
                     YEFSFESKGRESKIRRPASGAINHIQRLFRESSSENNTLHQYMNKIPCNTCGGELLCI
                     EARFTTIKGYRFPELTKMTIEQLWNWLCELPNHLQKNELSLVNDILTELKIRVSYLLK
                     VGLSYISTDRTAPTLSGGELQRVRLSSQLGSELVGLTYILDEPSIGLHPRDHNLIIKM
                     IEELRDKGNTVIVVEHDKDTILSADYIIDVGPSAGTKGGFIIAEGTTQEIIKNPNSIT
                     GKYLSTYNKISSQNKIIPSKWLSLKGCHANNLKNIDVEIPLNCMCSITGVSGSGKSSL
                     VFHSLLPALEEKLKQKSIPDKNYTEFTGFDAIDDFILMDQAPIGKSSRSTPATYINIF
                     DEIRSLFAETPQAKQKLLDESYFSFNSKKGQCPNCQGLGKTKIILQYMSDQWVTCSEC
                     QGKRYQKEILSIQYKGKTIADILDMEVAEAKTFFSDCSDIYRKLSLLDEVGLGYLKLG
                     QNTLGLSGGESQRIKLAKELGTKTKKRMLYILDEPTTGLHFKDIENLLITFRKLVNEQ
                     HSLLIIEHNTEVIRASDWIIDIGPDSGINGGEIVASGTPDEIKINPNSITGHFI"
     gene            15100..17334
                     /locus_tag="PC414_002242"
     CDS             15100..17334
                     /locus_tag="PC414_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2464635.1"
                     /translation="MKLSRYKKNSLVLSIILCSLIISILVITVFNTVKLHNISNKQSK
                     NYLNDVSTQIVMNVDSKIKFILSDLRIMADFIKQYEGDSRYEYLSKRKTSYKYYDVGI
                     IDLEGKAEFLSGKKFDLKNTVSYKKGIEGKEYSEVIESMGFVLYSVPIFNDNHEVSSI
                     LIGVSNKESMNEILNIDNFNGKGTIEIINSKGKPLFIGKNTELIKDLSHKYKSSGDEP
                     WAQKMLEDFKNDKSGNITITSSKGVQCLLTYHPITRGLNDWHFLLIVPEDAVLGELNK
                     LNSFTITMTFIITCIIGIITLILYIIRRKYVNQIENIAYLDSITNGINSTKFSMLVKP
                     LISESPDSTYMMIAMNIKDFKLINDCFGSEKGNMTLKHLYNILYKNIDENEEFVCRHD
                     ADLFYLLIKNRPVLEALELLYKIEADTNYFNKNRENPYFLRLSVGIYTIENHNEDLIT
                     IQDHANTARKSFNKTHQSDFSFYSDIERKRLISEKEISNLMEKALENKEFFMCLQPKI
                     DIKSGKISGAESLVRWKNSEKGIIYPSDFIPLFEKSGFICKLDLYILEETCKLISKWI
                     KEGKEVLRISVNVSRQHLNDKLFLEKYKHICNKYNVPTCLIDLELTESIFLENPEAID
                     IIEDIHSNGFKCSIDDFGFGYSSLGILKDFKVDIIKLDRSFFVSKNNIDRGKVVIKSI
                     IELSKRLGMKVTAEGIEELEQVEFLKNVGCDYIQGYVFSKPLLISDFENFAYENNKIK
                     NIHY"
     gene            17590..19620
                     /locus_tag="PC414_002243"
     CDS             17590..19620
                     /locus_tag="PC414_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2464636.1"
                     /translation="MISGRMLLIMNFLKGKNKTSYKDISKQLSIEERKVRYDINNLNI
                     VLRSLNKSVIQKMNKGVLIIPDDFQVVCINNNEYVFSLTERVSIMKIISMFKIDNLNL
                     EKLSKEFQVSRSSIKNDLNVLSSELERNQITITYDKSFKISGEEDIIYKQRLNILKSY
                     SYLFGKEKSDLSVFEKYLVNVIEKIFKGIYLIDIYNWSMNLLLQMGWTLNDESFTWYV
                     SNIFLFTWYIHSDEKHPLESASLSEPCFENKFDQEFETIIQKKLTKEQIFLLKSFVFF
                     TNKYASLNEDMDLISTEIIVQDLIYNMSEILEISFKEDMILYKGLLNHIAPLIERIKQ
                     NIQIYEELLHVIPDKYQYVLEATKVSIQKNRVLSQIKNENETILLAIHFLGSIHRNER
                     NNYKNILLVCGFGYGVIAMLKDSLVNDYQINIVESIPSYKLQDYKEWESIDFIITTSK
                     IVIDLPKTIIEINPFLQEEDYLKFEEKGIKRKNVLTNYMSIKKRLDFLEEDAQKRVLS
                     IICQELGYSDERILFRQLNLSDLIGIDTIRIIDGDMKWEDAVKISSNILANCDFVSAQ
                     YAENIIDILKNVGFYAVKDNEFALLHGNDSSLVKVSSISLLICKEAVSFGDKKVKIIF
                     CLASRDKKEQIPAIINLTKMVYKTNFIAMLESAKTKEEAEVLIHKYEKEVTV"
     gene            19617..20069
                     /locus_tag="PC414_002244"
     CDS             19617..20069
                     /locus_tag="PC414_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2464637.1"
                     /translation="MNKLVNVKCSFKIHVNSKEEAIISLVDVVSKEGYLIDKNQFLKD
                     VLKREETLSTYIGHGIGLPHSQSVGVKNSCITIGKLDTPIEWTEEGEKVDLIFLISVT
                     KDNENNLHLKILSKLARLLMHESFRNQIRCSDEQTVYNLIKEKIEEED"
     gene            20071..21126
                     /locus_tag="PC414_002245"
     CDS             20071..21126
                     /locus_tag="PC414_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HDF2464638.1"
                     /translation="MRILKFLQKHLMTATSYMIPFVVAGGILFALSVTLSGQAAVPET
                     GWLAKLNQIGAAGLALFIPILGGYIAFSMADKPGLAPGMIGAYLAKEVNAGFIGGIIA
                     GFIAGFVVLQLKKIKLAPTMRTLGSIFIYPLVGTLITGGIIVFLIGEPIASFMTWMTN
                     WLNGMSGVSKIPLGGILGGMIASDMGGPINKVAATFAQTQVDTLPYLMGGVGVAICIP
                     PIGLGLATLLFPKKFSKEERDSGKASLLMGCVGITEGAIPFATSDPVRVIPCIMAGSI
                     VGNIMAFLLGCLNHAPWGGLIVLPVVDNRLGYIASVLTGAVVVAVLMKLVKKDVKEDE
                     EIEEDLDESIELIFEEL"
     gene            21193..21501
                     /locus_tag="PC414_002246"
     CDS             21193..21501
                     /locus_tag="PC414_002246"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HDF2464639.1"
                     /translation="MKIVAVTSCPSGVAHTYMSAESLELSAKKFGIEIKVETQGSSGI
                     DNELSLKDIDEATCVILTNDVEIRNMERFKGKKVVRMSVSEIIKKSDALIKKIKDTFQ
                     "
     gene            21605..22870
                     /locus_tag="PC414_002247"
     CDS             21605..22870
                     /locus_tag="PC414_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HDF2464640.1"
                     /translation="MKKLPIKTVVQRLLNLQEEGKSATLLGIGPMSPNLLQASFELAK
                     DDDFPLMFIASRNQVDADELGGGYVNGWNQETFTKDIKKVADKVGFDGLYYLCRDHGG
                     PWQRDKERNDHLPVDEAMELGKKSYLADIEAGFDLLMIDPTKDPFEIGKVIPLDVVLE
                     RTVELIEYCENERKRLNLPDIGYEVGTEETNGGLTSTETYETFITRLKVELDNRGLPM
                     PTFIVGQTGTLTRKTEQVGTFNFRNAYDLAQMAKKYGVGLKEHNGDYLDDVTLLEHIP
                     SQIIATNVAPQYGTEETRAYLKLAEVECKLEKEGLVEKTSNIRHVLLVNAIECGRWRK
                     WVVGEQKNLTTEEIFKDEVLSNEILDIAGHYTFNNDDVKKEIEVLYDNLSKNNIDGQR
                     FVVDHIKRPLRDYAECYNLKGVTTRILNK"
     gene            22958..23674
                     /locus_tag="PC414_002248"
     CDS             22958..23674
                     /locus_tag="PC414_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDF2464641.1"
                     /translation="MINLIFDVDDTLYNQLTPFYTAYNKVFSSIKDISIEDLYMSSRK
                     YSDEVFHMTENGEMPIKEMHIYRIMKAFEELGNSITEKDAQSFQDEYIYQQSQITLIP
                     EVERILNFSKERNINLGIITNGPSNHQRMKLKQLNIENWVDKSNIFISSEVGFSKPDT
                     NIFRVAENVMNLDRENTYYVGDSYRNDVLGAKKAGWKSIWLNHRGHELEELFYKPDFV
                     ILEHKDLISLFIKICSYKNM"
     gene            complement(23869..24162)
                     /locus_tag="PC414_002249"
     CDS             complement(23869..24162)
                     /locus_tag="PC414_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HDF2464642.1"
                     /translation="MRNMIENLIKNNTYIDDKVIMLSMLSSAKASANMYLNSALTSST
                     PELRAIYSASLTQMVEGHTALTELSINKGWVKPYDKPIQQLTCSYKESQNVID"
     gene            complement(24168..24383)
                     /locus_tag="PC414_002250"
     CDS             complement(24168..24383)
                     /locus_tag="PC414_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464643.1"
                     /translation="MRILSETEKISLAAIIKMESDGLLMQRAINMLISDEDLKRQSES
                     SILATEGRIKAIQQFIVENEILISEEV"
BASE COUNT         9522 a         2802 c         3979 g         8493 t
ORIGIN      
        1 tgcttacaaa aatagaatat tttatagatg atgtgttaaa agaaactata acaacagatt
       61 taacaacaga gaaaacaatt aactatacac tagaagataa tgcactacac acacttaaga
      121 tagttgttac agacagtaat aatgctacag cagaaaaagt gttaagtata agtaagaata
      181 taatgccatt gcccgaagat gtgaatctgc aagacatatc ttctaaacta attgaagtta
      241 atgcaggatt taaaactgga aaaacaagta ttataaacac tttagcatta aaaaatatag
      301 aagcaaattt aaataataca ttagttgaat tatcagagaa aataaaaaca ggttttgata
      361 gttcagatgc tactgctgaa caacttcaaa atcagattac taatttaaat aatcagttaa
      421 gccaacgtaa aagatgggct aagggtactt acacttttac tcaaagtgat tatgaaaatt
      481 tttattcaag gtattcaagt gctgaaaatt atgctaagag tcttactgtt cctattaatt
      541 taaatttcat ccctagtctt gtcattgttg aaaaactttg tttttatgga aatgggtata
      601 ctgttaattt ttatgctatt aataatttaa atcaacttgc ttctgttggt cgtgttccta
      661 agagtagtgg agaatatcgt ttttgtgctt ttatgtttgt aaaaactatt actagcaaag
      721 aattttcttt atatacttat cgagatgaat acactgatag tagtagcgta gtataccgtt
      781 gtaaagtcgg agaaagtttt acatggtatg cttatgaata atatgaaagg tggtaattta
      841 aatggaaaga ggaaatagaa ttatatgtga ccaaaccggg aaaatattac tccaaactgg
      901 agaagcaaca ggggatatat tagaacatga tgaaataacg gaattacatt ttgttgatat
      961 tccgtatgga agtatagact atacaagaaa tagaattata ggtataaata tagaaacaaa
     1021 agaaccaatt ttagaggaaa taccaacatt tatttcagat gaagaaaaga gaatacaaga
     1081 gttagaaaat caaattttat taaatgaaaa tgagaaagta ggaggaattt tataatgaat
     1141 ataaataatg ttgtagtaag aatattagca gagagaatat taaataaggg attaaatcct
     1201 ctaaaaaata gagtatttga acttgatgat gttactaaca cagagtacag gatggcagtt
     1261 gaaaactaca ttatagaaca tagtgaggta gtagaaggag cagaacttac aaaataggta
     1321 atgttctttt tttaatacaa agcaatagga ggttttcatg aatgaagaac ttgtgaaaga
     1381 aaaaataagt acacttgaaa caagagtaaa agaacacggc aaacaaattg atagaatcga
     1441 gattgagcaa gcaaagtttg ctatacaaat acaaaaccta tgtagtgata taaaaaatct
     1501 gacaggagta ctcaagtggc ttgtaggagt tataattaca actttagtag ggttctttat
     1561 atttgccata cagaaaggaa tattttaatt aattaggagg ttaagatatg gataatttaa
     1621 taagttttat accagagcaa ctgcttttat tagtagttgc tcttaatgta ttaggatttg
     1681 gatttaagaa atacaaacag ttagataata aatacattcc aatcatattg cttgtgatag
     1741 gaattggatt ttcaatatgg atgttaggat ttaatccttc atcaatttta caaggaattt
     1801 tatgttgggg agttgcaata ggggcaaatc aagtttacaa acagttgaag gatggtgata
     1861 aataatgaaa atagcaatag taccaggaca cactttaagt ggaaaaggaa caggagcaac
     1921 tggctatata gacgaaggaa aagaaaatag aattttaact gatttaattg ttaaatggtt
     1981 gaaacaaggt ggagctattg tgtatactgg aaaagtagat aaatctaata actacttagc
     2041 agaacaatgt aaaattgcaa ataggcaaaa tgtagactta gcagtacaaa tccatttcaa
     2101 cgcaaataaa acaactctaa atcctatggg tacagagaca atatacaaaa ctaacaatgg
     2161 taaggtatat gccgaaagag ttaatgataa attagcaaca gtattcgaaa atagaggtgc
     2221 aaaatcagat gtaagaggtc tttactggct taagcataca aaagctccag cgatattgat
     2281 tgaggtgtgc ttcgtagata gtaaagcaga tacagattat tatattaaaa ataaaaatac
     2341 agttgcaaaa cttatagcag agggtatatt aaataaaaat ataaataaca aagagaatgg
     2401 tgagggaaaa atcatgtaca aacatacaat cgtttatgat ggagaagttg acaaaatacc
     2461 tgcaactgta gttggctggg gctataatga tgggaaaata ctgatatgtg atataaaaga
     2521 ttatgtacct ggccagacac aaaatcttta tgttgttggt ggtggagcat gtgaaaagat
     2581 aagttctatg actaaagaaa attatactat gataaaaggt aatgatagat ttgatacact
     2641 tcacaaagta ttagatttta ttgatagata gattagaagg tagcaactag ttagttgtta
     2701 ccttcttttt ttatgtctac ttttctttct ttttccaaac aacttcatat cctatcaaat
     2761 tagcaatagt caagatttca ctatacttca tagtctcatt atttattttt tgtcttaaat
     2821 tttcaactgt attattctta ttattatgtt cattcattaa attatttaac tcattcatgc
     2881 tgatattttc ttttacaaga atacttttaa tttcattctt ccatgtcatt ttttcgcacc
     2941 tctcttattt atataataac ataaaaaaat taaaagtaaa agaataatct acgctaatca
     3001 aagttatatt tttggaaatc aaagaaaaaa ctttgaaaaa gtgttgactt agatattcat
     3061 atagattata atataattaa caaataaaac aagggggtaa gcaaagaatg aaaattttat
     3121 ttaaagaagc gcataaaatg gcaagagaaa taaaaagaaa atatagtaat gtagattata
     3181 aagtacaatt tagtctttgc ttatcattct taaataaaaa gggggatata aaattgaaag
     3241 aattaaaagg gtcagaaaaa caaattattt gggctaatga tataagaaca gacattttaa
     3301 atttaactaa cgaactagaa aaaagtaaaa tagaaagaat aaaaaatgaa gactacaaaa
     3361 ttaaagacat gagtatagaa gaaatgacag aaagatgtaa aagaaaattt gaaagaataa
     3421 gagaagcaat aagcaatata gagaatgcta agttctttat agataatttt agaaatgttt
     3481 taaagtataa ttctttaaat caaaaagcat ttcatataaa tcagatatta aaagattctc
     3541 aatttgcaaa agagaacaga aattggagtt ttttacaagg ggagacacaa gcatcttata
     3601 gacttaaaaa agttaaagat tatagtataa gctatgaaga agctaagaaa atagctaaag
     3661 aaataaattt taatgataat tggtctaatg aaataaaaga aaatgtactt gaaatagttg
     3721 aaatatttga cgacgcagta aaacaagcta ctagcacaga atatatagag aaagctaaaa
     3781 aatacataat agaaacatta atacaaaaaa atgcacaatt ttatagaaat ggatttgcag
     3841 acattaaaaa gaagcagatt gaaaactata gaaaaataga aagtgatgaa agaaaaatag
     3901 aaattgcata ccaagtattc agagatggcg tagagtgtta aaagtttcaa cttgatacaa
     3961 atatagttca ccattgaatg ctataattaa aataaaaaat aattggaggt ttaaaacatg
     4021 aatatagatt ttagtacaat attagattta aaagttaata aagagaatgt taaaatgata
     4081 gaaaaagaga ttaataaaat caacaatgat atatttgata taagtcttaa agattgtgcg
     4141 aacggtcaca ttggaagcgt taaagaaaca gttggaaaaa gtttaaacat tgaaacgtgg
     4201 ggtcatggtg aaattaacag taaaatagga ataagattga gatttaattt ttattattca
     4261 aaaggcgaca aagtagacat agaaaatata gaaaacattt taatatatgt aaatagcttg
     4321 tgtagcaaaa atgtagaaag tgaccttata gccaatgtaa gacctaaatt ttataaatta
     4381 gaagagaaat attctacacg tattgaaaaa tgtgaaatta gacacgaatt tataggcata
     4441 aacaagcaag aaattaaaga atttttagag tatcttacag aaattgaaaa gagcgaaaga
     4501 actgttatat ataaaaaata actaaaaaat ggaggaaatt aaaatggata aaatggataa
     4561 aaaagaagct aaaaaatttt taaaggttat agatatgaat atagataaaa tagaggaaga
     4621 agctataaaa gcttttaaag aaagttgttt aatcaaagag actaatgata taaaaattta
     4681 tatcgatata caaggaaaag ttgaagcgat agcaatccag gcttatgcta aacttttaga
     4741 tgatgataaa gaaattaata ttttcacatc aagacaaaca ggaaataact tagacgatat
     4801 gtttggagaa atttactacg taaatgatta cgaagaattt gaaaattggt gcgaaaataa
     4861 atgcgaaagt ttgaattggg atagttataa aaaattcaat aaagaaaatt ttgaagaaat
     4921 tgctgaaaga aatatagacg atagcacatc agttttttta gaagaattac gagaaggcat
     4981 tgaaagttgt aaacaagaat tacaaaatat agttgaaaat taagtagaag agtattatta
     5041 ttaatgtata tagtatcgct tttaagatta actacgaggg atttaaaata ttttttaatt
     5101 tatttagaaa taatattgta ataagaattt ttatattaac tacaagggat ttaaatgtca
     5161 atttaattta tagagaaatg atataatggt attttttaga ttaactataa gggatttaaa
     5221 tatcaattta atctatattc acatgatata atatcacatt attttatatt aactatgcgg
     5281 aaataaaagc agtgtattta ataaatacac tgctttttta atgctaaaga tttagtcatt
     5341 atgataactt aatccttcga ttataaatcc tacattcgtc gtcatttctg cttcatcact
     5401 ttgtaatatt ccttctgtaa attcgctatc gcttgataat gttatttttt tccttgccac
     5461 catttccaaa attgtttgaa atagtttaat tttcatattc ttatcaagtc cagcgtattg
     5521 caaatagttt gctagctcct gcgaaaactt atttaattca gatttagtca tttttttaga
     5581 taattcttct ccaatattta gataaacttt tttagattta gattgttcaa cattcatttt
     5641 tttcaacccc ttttttaagt tataattcaa ttatatttta aaaatgctaa tacttcaaat
     5701 ataaatattt ttgtttaagt atataaaaca tcaataacat tactcaacac accttaaaat
     5761 tgatttaaat agctcttttt tatgaacaaa attaatgata taataaaaaa tagaagtgta
     5821 ttggtagtac acttctatag ttataactaa gcattctctt gctggggagt gcttttttca
     5881 tttttccaaa caacattatt tttttcatca ataattatta aatcaatctc attatctttt
     5941 aaaatctctg ttgcagtaca aatgagatta tctaagtcga aactacgagt tatctctagt
     6001 tgattgttat ttacaactct tttaagtaca taagtcttaa agcacattta tattattatc
     6061 ctttctcaca aaaatacttt aattaatttt ttaacatagt aatatttaaa agtagataaa
     6121 actaaaaagg gttctctaga aatcaatgtc tatctaatta tacttactta ccaataccat
     6181 cacttggtgt aaacaagact cactacattt tctccgtact ctaatataaa atcatctaac
     6241 tgtgtatcta aaaaattata ataatcatac gaactgctaa tataagcagt caatacatgt
     6301 gtacatttat ccattttatt tttaaacata taaattatta tacccctaaa aaactttcat
     6361 aaaaacaaaa gaacaatatc tctttggcaa ctggctgaca taactcataa gatatttata
     6421 taagtcttag tccctatagc tttgcgtcac taaatttctc tagttttgcc gatttagttt
     6481 tattctacaa ctaaaataat acaatagaat tagttattat tcaacaaaat tatttgaaaa
     6541 tttaataatt ttatagatat gaaagttatt ttcttctaag aaaacagact aaatgcgtag
     6601 aatatttggt aaaacttacc tgtataaatt gaaatcagaa ggttttatta ttgatttaga
     6661 ttagaacaat ttgagggagt gacataaaat gaaacaacaa gaagtagtta ataaactacg
     6721 caggggagaa ttgatgttga tggaatactt gtggaggaaa aattctattt tatctaaaaa
     6781 agaaataata gaagcaatga aagaaaaata taagtggagg aaaagtacta cagaaattct
     6841 attaaaaaga ttagtaaaaa tgaaaacatt aaaaaagaag aggataggtt ttcagtttag
     6901 ttatgaagtg ctagtaacta aaaaagaata tttaaatgca ataaaagaag atagaaaggt
     6961 aactaaatat gatagttttt ttatacgaat gtttaccact atgcataaaa aagaaaagat
     7021 gacagaagaa caaataaaaa aatatataga aaatatggaa aaaattggaa aagaataatt
     7081 tatatatata ttaattattt ttaaaataaa tttgttataa tatttaaata tacataaaca
     7141 tacataaatt tacaaaaagg gggtgtagct tatgggattc aagaaattgc cagattctga
     7201 gttaaaagta atgaagttta tttggggatt agatggaaat aaggtagcat caggagaagt
     7261 tgtggaagca atgaaacaaa tttatgactg gaataaaaat acgacattaa caatattgtc
     7321 taagcttgca aagaaaaact atttgtattc tcaaaaaaca agcaagtgta catattatac
     7381 tatcgctgta agagaaaaag attatttaaa agttgaaaca aaaaaattct ttagtttttt
     7441 tcataataat tctttaaaaa gttttttcac tgctttaaat gatgaagaaa atttaagtga
     7501 tgataaatta gatatgttag aggaatgggt gaaaaattgg gaagaagatg aataaaatga
     7561 atgtgatact aaaatgatac taatatgttt tatagcgtgt taaaaaatat agaaaatata
     7621 gaaataatag aagaaaatta atgtaataga taaggtttta tcagattaaa aagaaaatca
     7681 atagaatggg aataaaatat agaatgccaa aaatccacat aaatacagga tttaaggtct
     7741 ctcttacttc tatgttatac tggtttgata ctatcaatat ttgccagtat cttatggatt
     7801 aataagtaaa aaacatcctg ctgaatgata aattcagcgg ggtgttttta ttttgtttta
     7861 gaactccttt gctctgaaac tcgaagtatt taacactgat gattttgtta tcttttagtt
     7921 ccttttgtgt catgctctga aagacaaatt gatacaataa aaaagtaatt tcttaatcat
     7981 tgatggagat atgtatttat gttactcttg atggtaattt gagtaatatt gttcatagct
     8041 ttttatattc aattaattag aaaaccattt ggtataatca taaataaact tgatgatata
     8101 atagaaaaga tttataatta ttagaagtat ataaaagtat tttatgtcta tgaaatgtat
     8161 gtctataaaa catatagatg acttttgtat attaatgtat gaatattgat attatagaag
     8221 aattgagttg tcaatgatac taaatcaaat atttatagaa gctggaggga ttatgagaaa
     8281 taaaataatt ataggaatta cttttttact tgtattacta atgtctggat gtgcttcaaa
     8341 acaaaccgca gaaataaacg tattagcaac tactgattta catggagtta taccttatga
     8401 gttaacttct tatgtaaaag aagaaagaaa aaaagataaa aatctaactt tagtagatgc
     8461 aggagatttt tttgatagtg gagaagtgtt tggttctagc atggatagtt attttagtgc
     8521 tagatataaa gatggtgata ataacactga aagttatatt gagacgccta tttcaaaaga
     8581 tatgaaagag gtaggttatg atgttgcagt tcttgggaat catgaattta tatccaacaa
     8641 taaattttat ctagataata tgatatctga ttttgagaag caaaaaataa atattttatc
     8701 ggcaaatacg tataaaaaaa atggagacag ttatacaaaa ccatacataa taaaaaatat
     8761 taatacacct gaaggaaacg taaaactagg aattttaggg cttactataa aagaagttgg
     8821 tgagaggaaa cagtggaaag atggaaaact tgttgactct aagtctttag aactaaaaga
     8881 ccaagagggc tacaatggtg aattgtatat gaatgattta gtagaagatg ctaaaaagtg
     8941 ggttagtata atggaaaaag agaagcctga cattatagtt gctgtggcac atacaggtga
     9001 gaaacctaag aaacctaaaa acccaggaaa tagaatacaa gacttagctc aacaagttga
     9061 tggaattgat gctatagttg caggtcataa tcatgttcaa attgagcaac atgattataa
     9121 aaataaatca ggagaaaatg ttatagttac tgagccagga aagcatgggg aatgtatttc
     9181 taagattaat tttaaattag aaaaaaacaa tgataaatgg aatatagttg acaaatctag
     9241 taaattaatt aaatttgaaa aaaatcaaga aagtgataaa tttggagaat ttatttcttc
     9301 aattaataaa gtaagtaaag ataagaaaga aataagatta tcagaagtaa agactcctga
     9361 atgggataaa atatatgttt ttaaatctgg aacatctgca gatgatatat ataaaacaat
     9421 aggatataaa tggagaaata tatcatctcc aaagagtgat ggcatggtgc agattgttgt
     9481 aatgaataat aataagcctg ttagctatat atatggtgaa gctgaagtta tgccaattaa
     9541 tataaaatgt gatgaatcag agtataaaga taatgttgta actatttatc ctaataaaaa
     9601 tgataaattt aaaattgaaa aaggtaaaca agaatatagt attaatttaa aacatataca
     9661 atagaaactt ttatcttatt taagtttaga ttgtacttaa aataaagtgt gtattatttt
     9721 aaaaattgat gataattaaa atcacaacat taaggaatag tacacacttt ttatgctaaa
     9781 aaatagaatg ttaaaaaatt aaacctttaa attaaagtac attgcttata ttatccaata
     9841 ttatcttcaa aggttgtgta atatttttat ccttatgtaa tattgctaac attttatttt
     9901 ccatatctat cccatctaat tcaatcaatc gcaatttttt atgtaagagt gaatctaata
     9961 ataaattttc tggcaaaatg gttataccta accctgcttg tgcagcttta ataagtgcaa
    10021 aagaattaac actttcccaa acaggataag ctttttggtt tgaaagagat aatacactgt
    10081 ccaaagtatc gcgaatggca cttccttgct cacgtaacaa taacggaaac gtgcataact
    10141 gtcgtagaga taaggcttgt ttagtattgg tggtggttat gcaatcatac ctttaatcca
    10201 agagaaagta gtaaatcaaa ctggatggat aagtgagaaa atatttacag atattataac
    10261 tatttcacaa atgacaccag ggccactggc tgttaatact tctacttttg ttggacttca
    10321 aattggaggg ttttggggag cagttgcagc cactttaggt tgtgtattat gtggtgtagt
    10381 aatatctcta tttttatata atttttttca aaagcatcag aactctactt atatatttga
    10441 aatattaaat ggtctaaaat cagcttcatt aggtttaatt atctcagctg cggctacaat
    10501 tataattctt gcattatttg ggacgaataa agtgtacttc aattctatat ttgagatgct
    10561 aaactggact gcactaataa tcttttttgt tacactactg atagtacgta agtggaaaat
    10621 taatcctatt gtgattataa taatgagtgg tatactggga atattttttt atttataagt
    10681 ttttgctaag taaaggatat ctattttttt atatcttaaa tccaaatagc caataaagta
    10741 aggctatttg gatttttaat aaattctgaa aagataatca gaaacctata taacatttaa
    10801 attatatcac tggttttcta caaaccaatc ataagcttct tggcaaaatt tcttactatc
    10861 tatatagtaa ctattatgaa cattattaat ttcaacaagt ttctttttac attttagtga
    10921 attataatag ctatcaaaat attgggtttt aaaatatcta tcatgaagtg ttttaaagac
    10981 aagagtaggt atgttgactc catcttttat cttactatct tttgcaataa agcattctaa
    11041 cattgtatta attatctttg tactattttt ccaaatatga ggatttttct tttcaaatat
    11101 tttataaaat tcattatcat agacttcatt ataaccttta tatttaggtg gttttactgt
    11161 tatgtgaaaa ttaggaagaa acttaaagaa aaaatataat aatttttatg ttctgtttta
    11221 tatattattc atattttatc acagataata tactaaagtt aattttttta ttgtacaact
    11281 attataataa ttcttctata atataagaca ttctataatt ttatatttaa gttcatgtgt
    11341 atttaattgt ttttatataa aatgtccagt aatactattt ggatttattt ttatttcatc
    11401 aggagttcca gaagcaacaa tttcccctcc atttattcca ctgtctggac ctatatcgat
    11461 aatccaatca gaagcacgta taacctctgt attgtgttca ataattaaaa gtgaatgctg
    11521 ttcatttaca agttttctaa aagtaattaa caaattttct atatctttaa aatgaagtcc
    11581 agtagtaggc tcatccaata tgtacagcat tcgtttttta gtttttgtcc ctaattcttt
    11641 tgctaatttt atcctttgag attctcctcc acttaatcct aatgtatttt gtccaagctt
    11701 tagatatcca agtcccactt catctaataa ggagagtttt ctatatatat cagagcaatc
    11761 gctaaaaaat gtttttgctt ctgctacttc catgtcaagt atatctgcaa ttgtctttcc
    11821 cttgtattgt atagacagta tttctttttg atatctctta ccttgacatt cactacaagt
    11881 tacccattgg tctgacatat attgcagaat aatttttgtt ttacctaatc cttgacagtt
    11941 tgggcattgc ccctttttgc tattaaagct aaaatagctt tcatccaata atttttgttt
    12001 tgcttgtgga gtttcagcaa atagagaacg aatttcatca aaaatattga tatatgtagc
    12061 aggtgtagaa cggctagatt ttccaatagg tgcttggtcc attaagataa aatcatcaat
    12121 agcatcaaat ccagtaaatt cagtataatt tttatctggg atagattttt gtttcaattt
    12181 ttcttctaga gcaggaagta aactgtgaaa tacaagagag ctcttaccag aaccactaac
    12241 acctgtaata gagcacatac agtttaaggg aatttctaca tcaatatttt ttaagttatt
    12301 tgcatggcat ccttttaagc ttaaccattt acttggtata attttatttt gtgaacttat
    12361 tttattgtat gtacttaaat atttacctgt tatagagttt ggatttttta taatttcttg
    12421 ggttgtacct tctgcgataa tgaacccccc ttttgtacca gcacttgggc ctacatcaat
    12481 aatataatca gcagacagta ttgtatcttt atcgtgttcc acaactataa cagtattacc
    12541 cttatctctt agctcttcta tcattttgat gattaaatta tggtctcttg ggtgtaaacc
    12601 aatagatggt tcatctaaaa tgtaggttaa gcctactaat tcacttccta gctgacttga
    12661 taatcgtact ctctgcagtt ctcctccaga taaagttgga gctgttctat ctgttgatat
    12721 atagctaaga cctaccttta atagataact aactcgaatt tttaattctg ttaagatatc
    12781 atttaccaaa gagagctcat ttttttgaag gtgatttggt aattcacaca accaattcca
    12841 taactgttct atcgtcattt ttgttagttc agggaagcga taacctttga ttgtagtgaa
    12901 tctagcctca atgcacagca attcgccacc acatgtattg caaggaattt tgttcatata
    12961 ttgatgcaag gtattatttt cagaactaga ttctctaaaa agtctttgaa tgtggttgat
    13021 tgctcctgaa gcaggtcttc taatctttga ttctcgtccc tttgattcaa agctaaattc
    13081 atatatctta tcgtctgtac catataagat tgcatctata aaatctcttg gtaattcatt
    13141 ccaaggtata tctaggtcta tatttttatc ttttgctatt gcatataatt cacctaccat
    13201 ccaattacca tttggctttt tccccctcaa ttttccaaaa tagtaagtag ctccatctaa
    13261 tattgaaata gttgggtctt ttacaattaa ttgaggatta acttgatagg taaaacctaa
    13321 tccattacaa tcgtgacaag ctccagtatg ggagttagca ttgaaggttg aagtatttat
    13381 cttagaaaaa atagtttgac aatgtggaca ataatttttt gtattattct ttttttctaa
    13441 tggaattttg caatatggac agtaactttt tccgattgat acaaataaca ttcttaaata
    13501 atcccaaatg cttgtaagag taccaacagt agagcgagga ttttggcttc cttttttctg
    13561 ttcgatggct atagtagggg ttaatccctg aagtaaatca aattccggtt tttgaagctg
    13621 ttctgaaagt tttgctccgt tacttagatt ttcaaaataa cgtctttttc cttcttcata
    13681 aatcgtatcg aaagctaaag aagattttcc actaccactt acacctgtaa taacagttat
    13741 ttgatttttt ggaattgtaa cttcaacatc ctttaaattg ttttgccttc ctccttttaa
    13801 atagatagag cttttattat gacaaagtga atttttaatt gatttattta ttgcttttgt
    13861 aaatttgaat ttcttttcta gttcaagagg attgcctaat tcttttattt ccgaaatacc
    13921 taatattttt aactgagaaa agattgattt caaataatct tcaggcaatt tttctattaa
    13981 caaattaagg gcttctattt ttatatttcc gcttgataat atctctgcca tttcatctaa
    14041 taaatttttt tctgctcttg ttaattgatt gtattttttg gaacttaatt ctgtccataa
    14101 atcccatgga agcaattcta aaccaatcat attattaaag tcatgtagaa gattaccctt
    14161 tacagaatgc caacctttcc aatttttatt atatccaaag tcatctggat gagcatgtcc
    14221 ttgccgacat cttatccagg cttctgaacc agttaaaaaa ccatctgttt ttccgacatc
    14281 atgagtgtca aaattaattt gaaaataatt tttttgtatg tcatctattt gtgcatctat
    14341 tcttatccat ctctttgtta gagtatcata gtattctaca agccagtggt cttcataagt
    14401 aagctcactt tcaaaataat tagcaaaacc tgctctcatt ctagcctcaa aacctcgata
    14461 tcttaggagc gatacaagaa gtaaagaata atctctgcac attccaacaa gcctttgttt
    14521 tgggtttctt ggaatagtta gtgggttaga atttatttca aatattcttt ttaacatttg
    14581 aggaattgta cgaagcaatt cctcatcaga ttgttgctta ttaaaggata taccatataa
    14641 ttttccttgg tctccatgaa ttactaggct ttgaaccact tttaataact caattggagt
    14701 ctttggtaaa tcttgatatt tattttcaaa ttttttagga tttgtgatga tactttgatt
    14761 tttatagtta tttattttca tttatatttc acctctctaa atggatgata taccctgaca
    14821 tggtgtcata tgcaaggaca atttagattt ttggccaaaa tttaaaaaat aaataaaaaa
    14881 tgatacagtg ataaattgta atacaaatat agtttcaaat tgcatataat ataattattt
    14941 aataaaacca ttatctattg aatgttaatg aaatgttaag atggcattct tgaatattaa
    15001 gaaatagaat actataatat agtgtgaagg atttttagta aaaaaatttg taagtgttaa
    15061 tatacattaa aaaataggtt tataatttgg aggaaaaaaa tgaaattatc aaggtataag
    15121 aaaaatagtt tagttctgtc tataatttta tgttcactta ttatttcaat ccttgtaatt
    15181 actgttttta ataccgtaaa actacacaat atatctaata aacaatctaa aaactattta
    15241 aatgatgtat ctacacagat tgtaatgaat gttgattcta aaatcaaatt tattttatcc
    15301 gacttaagaa taatggcaga ttttattaag caatatgaag gtgatagtcg atatgagtat
    15361 ttatctaaaa gaaagactag ttataagtat tatgatgttg gaataataga tttagaaggt
    15421 aaagctgagt ttttaagtgg aaaaaagttt gacttaaaga atactgtttc ctataaaaaa
    15481 ggaattgaag gtaaagaata ctcagaagta atagagtcaa tgggttttgt tctttatagt
    15541 gtacctatat ttaatgataa tcatgaagta tctagtattt taataggtgt atccaataaa
    15601 gaaagtatga atgagattct taatatagat aactttaatg gaaaaggaac tattgaaatt
    15661 attaatagta agggaaaacc tcttttcata ggaaaaaata ctgaattaat aaaggacctt
    15721 agccataaat ataagagttc tggagatgaa ccttgggcac aaaagatgct tgaggatttt
    15781 aaaaatgata aatcagggaa tattacaata acctcatcaa aaggtgtaca atgcttatta
    15841 acttatcatc caattacaag aggtttaaat gattggcatt ttcttctcat agtacctgaa
    15901 gatgctgtac ttggtgaact taataaatta aactctttta caataacaat gacatttata
    15961 attacatgta ttattggaat tataacatta attttatata ttataagaag aaaatatgta
    16021 aatcaaatag aaaatattgc atatttagat agtataacaa atggtataaa ttcaactaag
    16081 tttagtatgc tagtcaaacc tttaatttca gaatcacctg atagtactta tatgatgata
    16141 gcaatgaata taaaagattt taagctaata aatgattgtt tcggtagtga aaagggaaat
    16201 atgacactaa aacatcttta caatattttg tataaaaaca ttgatgaaaa tgaagaattt
    16261 gtgtgccgtc atgatgcaga ccttttctat ttgcttatta agaatagacc agtgttagaa
    16321 gccctggagt tattatataa aatagaagct gacacaaatt attttaataa aaatagggaa
    16381 aatccatact ttttaagact atcagtaggt atttacacaa ttgaaaatca taatgaagat
    16441 ttaattacaa tacaagacca tgcaaataca gcaagaaaaa gctttaataa aactcatcaa
    16501 agtgattttt ctttttatag tgatatagaa agaaaacgat taatatctga aaaggaaatt
    16561 tcaaatttaa tggaaaaagc ccttgaaaat aaagaatttt ttatgtgctt acaacctaaa
    16621 attgatataa aaagtggaaa aattagtgga gcagagtctc ttgtgcgttg gaaaaattca
    16681 gaaaagggca taatttatcc aagtgatttt attcctcttt tcgaaaaaag tggatttata
    16741 tgtaagttag atttatatat actagaagaa acatgcaaat tgatttctaa gtggataaaa
    16801 gaaggtaaag aagtgttgag aatttctgtt aatgtatcac gtcaacatct gaatgataaa
    16861 ttgtttttag aaaaatataa gcatatatgc aataaataca atgtaccaac atgtctgata
    16921 gatttagaac ttaccgaatc tattttctta gaaaatccag aagctataga tataatagag
    16981 gatattcatt ctaatggatt taaatgttct atagacgatt ttggatttgg gtactcatca
    17041 ttaggaatat taaaggattt taaagttgac ataattaagt tagaccgttc attctttgtt
    17101 agcaaaaata atatagaccg tggtaaggta gttattaagt ctattattga acttagcaaa
    17161 aggctaggta tgaaagtaac tgctgaagga attgaagaat tagaacaagt tgagtttcta
    17221 aaaaatgtgg gatgtgatta tattcaaggt tatgtttttt caaaaccatt attaatttct
    17281 gattttgaaa attttgcata tgaaaataat aaaattaaaa atatccatta ttaaatgtac
    17341 aagataaaaa atttaggcaa gttatataat taaaaatata acttgtcttt tttatttata
    17401 tatttaataa aaatatattt gcagattttg taaaaataat tttcaaatgt tgtaagttgt
    17461 ggctaaagct ttattgtatt attctgaata tagaaagata gttactgtct tagataaaaa
    17521 ctgaaacata cataataata taaaatttgc tatattaata actctaatta ttttggaagg
    17581 agggaaaata tgataagtgg gagaatgtta ctcatcatga attttctaaa aggaaaaaat
    17641 aagacaagtt acaaggatat tagcaaacaa cttagtatag aagaaagaaa agttagatat
    17701 gatataaata atttaaatat tgttttgcgt tcattaaaca agtcagtaat acaaaagatg
    17761 aacaaaggtg tgttaattat tcctgatgac ttccaagtag tatgtataaa taataacgaa
    17821 tatgtatttt ccttaactga gcgagtatct attatgaaaa ttatatcaat gtttaaaata
    17881 gataatctaa acttagaaaa attaagtaaa gaatttcagg tttctagaag ttcaattaaa
    17941 aatgacctta atgttttgtc aagtgaatta gaaagaaacc aaataactat tacttatgat
    18001 aaatctttta aaataagtgg agaagaagac attatatata agcaaagatt gaacatatta
    18061 aaatcgtatt cttatctttt tggtaaggaa aaatctgatt taagtgtatt tgagaagtat
    18121 ttagtgaatg taattgaaaa aatattcaaa gggatttatt tgattgatat atataattgg
    18181 tctatgaatt tattattgca aatgggatgg actttaaatg atgaatcttt tacatggtat
    18241 gtgtcaaaca ttttcctttt tacatggtat atacattcag atgaaaaaca tcctttagaa
    18301 tctgcttcat taagtgagcc gtgttttgaa aataagtttg accaagaatt tgaaacaatt
    18361 attcaaaaaa agttgactaa ggaacaaatt tttctgttaa agagttttgt attttttaca
    18421 aataaatatg caagtttaaa cgaggatatg gatttgatta gtacagaaat tatagttcaa
    18481 gatctaattt acaatatgtc agaaatatta gagattagct ttaaagaaga tatgattctt
    18541 tataaaggat tactcaatca tattgcccca ttgatagaga gaatcaaaca gaatatacag
    18601 atttatgagg aattacttca tgtaatacca gataaatatc aatatgtact tgaagctact
    18661 aaggtttcta ttcaaaaaaa cagagtttta agtcaaataa agaatgaaaa tgaaacaata
    18721 cttttggcaa tacatttctt aggaagtata catagaaatg agaggaataa ctacaaaaat
    18781 atattgttag tttgtggatt tggatatggt gtaattgcta tgttaaagga tagtttagta
    18841 aatgattacc agataaatat agtagagagt attccatcat ataagttaca agattataaa
    18901 gaatgggaaa gtattgactt cattataaca acatcaaaga tagtaataga cttgcctaaa
    18961 acaattattg agataaatcc atttttacaa gaagaggatt atttgaagtt tgaagaaaaa
    19021 gggataaaaa gaaagaacgt cttaactaat tatatgtcaa taaaaaaaag gttggacttt
    19081 ttagaagaag atgcccaaaa aagagtatta agtattatat gtcaagaact tggatacagt
    19141 gatgagagaa tattatttag acaacttaat ttaagtgatt taataggaat tgataccata
    19201 aggataattg atggagatat gaaatgggaa gatgctgtaa aaattagttc aaacatactt
    19261 gctaattgtg attttgtatc agcacaatat gctgagaata ttatagatat tttaaaaaat
    19321 gtgggttttt atgctgtaaa agataacgaa tttgctttac tacatggcaa tgatagttca
    19381 ttagtcaaag taagttcgat aagtttatta atatgtaaag aagcagttag ttttggagat
    19441 aaaaaggtta aaatcatatt ttgcctagca agcagagata aaaaagagca aattcctgca
    19501 ataattaatc taactaaaat ggtttataag actaatttta ttgctatgtt agaaagtgca
    19561 aagacaaaag aagaagcaga agttttaatt cataaatatg agaaggaggt cactgtatga
    19621 ataaattagt taatgtaaaa tgttcattta aaatacatgt taattcaaaa gaagaagcca
    19681 ttatatcgtt agttgatgta gtgtcaaaag aaggatattt aatagataaa aatcaatttt
    19741 taaaagatgt tttaaagaga gaagaaacat tgtcgactta tattggacat ggtataggtc
    19801 ttcctcatag tcaaagtgta ggtgttaaaa actcttgtat tacaatagga aagcttgata
    19861 ctcctattga atggacagaa gaaggagaaa aagtggattt aatatttttg atatcagtaa
    19921 caaaagacaa tgaaaataat cttcatttaa agattttatc aaaattagca cgtttattga
    19981 tgcatgaaag ttttagaaat caaattagat gttcagatga acaaactgtt tataatctta
    20041 taaaagaaaa aatagaggag gaagattaaa atgagaattt taaagttttt gcaaaaacat
    20101 cttatgacag caacatctta tatgattcca tttgtggttg caggaggaat attatttgct
    20161 ttatcagtaa ctttaagtgg acaagctgct gttcctgaaa caggctggtt ggctaaactg
    20221 aatcaaattg gagctgctgg cttggcttta ttcattccta tattaggtgg atatatagca
    20281 tttagtatgg cagataaacc aggattagct ccaggtatga taggtgcata tttagcaaaa
    20341 gaagtaaatg caggttttat aggtggtatc atagcaggat ttatagcagg atttgttgtt
    20401 ttacaattaa agaaaattaa attagctcct acaatgagaa ctttaggttc tatatttatt
    20461 tatcctttag ttggtacatt gattacaggt ggtataattg tatttttaat tggagaacct
    20521 atagcttctt ttatgacatg gatgacaaac tggttaaatg gaatgtctgg tgtttctaaa
    20581 attcctttag gtggaatctt aggaggaatg atagcctcag atatgggtgg ccctataaat
    20641 aaggttgctg ctacttttgc ccaaactcaa gttgacacac ttccatattt aatgggtgga
    20701 gttggcgttg caatttgtat acctccaatt ggtttaggat tagctacatt attgtttcca
    20761 aaaaaattta gcaaggaaga aagagattca ggtaaagctt cactattaat gggatgtgtt
    20821 ggaattacag aaggagctat tccatttgca acatcagatc cagttagagt aataccttgt
    20881 attatggctg ggtcgattgt aggaaatatt atggcttttt tattaggatg tttaaatcat
    20941 gcaccctggg gaggtctgat agtgttacct gtagttgaca ataggttggg gtatattgca
    21001 tcagtattaa ctggagcagt agttgttgcg gtattaatga aattagtaaa aaaagacgtg
    21061 aaagaagatg aagaaattga agaagattta gatgaatcaa ttgaattaat atttgaagaa
    21121 ttataaatat aaatggaagg aatttgtata aaaaagtaaa tataaaacta taaatttaaa
    21181 ggagataaga atatgaaaat tgtagcagta acatcttgtc ctagtggagt agcacatact
    21241 tacatgtcgg ctgaatcact tgaattatca gccaaaaaat ttggtataga aataaaggtt
    21301 gagactcaag gaagctctgg aatagataat gaattgtcac ttaaagatat tgatgaagca
    21361 acttgtgtaa tattgactaa tgatgttgag attagaaata tggaacgttt taaaggaaag
    21421 aaggtagtaa gaatgagtgt atctgagata attaagaaat ctgatgcatt aattaagaaa
    21481 ataaaagata cttttcaata aaaattgatt tattttacat ataactcaaa gatgttttta
    21541 aagaaatttt tattaaaagc atctttgaaa atatcttaaa aatagaataa aaggagatat
    21601 agtcatgaaa aagttaccaa taaagactgt cgtgcaaaga ttactaaact tacaagaaga
    21661 ggggaaaagt gcaactcttt taggaattgg tcctatgtca ccaaacttgt tacaagcaag
    21721 ttttgaacta gcaaaagatg atgattttcc tttaatgttt atcgcaagta gaaatcaagt
    21781 agatgcagat gaactaggtg gcggttatgt aaatggttgg aatcaggaaa cttttacaaa
    21841 agatattaag aaggttgccg acaaagttgg atttgatggt ttatattatt tatgtagaga
    21901 ccatggtggg ccatggcaaa gagataaaga gagaaatgac catttgcctg ttgatgaagc
    21961 aatggagtta ggaaaaaaat cttatttagc ggacattgaa gcaggttttg acctattgat
    22021 gattgaccct acaaaagacc catttgaaat tggaaaagta attccactag atgtagtact
    22081 agaaagaact gtagaattaa ttgagtattg tgaaaacgag agaaaaagac ttaatctacc
    22141 agacataggt tatgaggttg gtacagagga aaccaatgga ggtctgactt caactgagac
    22201 gtatgaaaca tttataacta gactaaaggt tgaactcgat aatagaggtt tgccaatgcc
    22261 aacatttata gttggtcaaa ctggtacttt aactagaaag acagaacaag taggaacttt
    22321 taattttaga aatgcttatg atttagctca aatggctaag aaatatggag ttggtttaaa
    22381 agagcataat ggcgattact tagatgatgt aactttatta gaacatatac catctcaaat
    22441 tattgctaca aatgtagctc ctcaatatgg tactgaagaa actagggcat atttaaaatt
    22501 agcagaagtt gagtgtaaat tagaaaaaga gggcttagtt gagaaaactt ctaatatcag
    22561 acatgttctt cttgttaatg caattgaatg tggacgttgg agaaaatggg tagtaggaga
    22621 acaaaaaaat ctaactactg aagagatatt taaggatgaa gttttatcta atgaaatact
    22681 agatattgca ggacattata catttaataa tgatgatgtt aaaaaagaaa tagaagtgct
    22741 ttatgataat ttaagtaaga ataatattga tggtcaaaga tttgttgtag accatattaa
    22801 acgtcctttg cgtgattatg cagaatgcta taacttaaaa ggtgttacta cgaggatttt
    22861 aaataaataa taggacttat agtggtgagt attgttatac taggtattta tagttataat
    22921 tatacttacc actattaaat ttaggaggac taatattttg attaatttaa tttttgatgt
    22981 agatgatact ttatataatc aattaacgcc tttttacact gcatataata aagtgttttc
    23041 ttcaataaag gatatttcta ttgaagattt atatatgagt agtagaaagt atagtgatga
    23101 ggtatttcat atgactgaaa atggagaaat gccaatcaaa gaaatgcata tttataggat
    23161 aatgaaagct tttgaggagt tgggaaattc tattacagaa aaagatgcac aaagttttca
    23221 agatgagtat atatatcaac aatcacaaat tacactaata ccagaagttg agaggatttt
    23281 aaatttttct aaagaaagaa atattaatct aggaattata actaatggcc catcaaacca
    23341 tcaaagaatg aagttaaagc agttaaatat tgaaaactgg gttgataaaa gtaatatttt
    23401 tatttctagt gaggttggat tttctaagcc agatactaat atttttagag tagctgagaa
    23461 tgttatgaat ttagatagag aaaatacata ttatgtgggt gattcttata gaaatgatgt
    23521 attaggtgct aaaaaagctg gttggaagag tatatggttg aaccataggg gacatgagtt
    23581 agaagagttg ttttataaac ctgattttgt aattttggaa cataaggatt tgatatcttt
    23641 atttataaaa atatgtagtt ataaaaatat gtagtaaaaa aatgttaata atagaattgt
    23701 ataaaataat taataataac tatataaaat tatatgttta gattgtgagc aatctatttt
    23761 atgttaaaaa tagattattt agaagaaggg gaaatcttaa aaaaatttga atatcccctt
    23821 tttattattt aattaaaatg aaatagttag aaatatattt tatttagttt agtcaataac
    23881 attttgggat tctttataac tacatgttaa ctgttgaata ggtttatcat atggtttaac
    23941 ccatccttta ttaatactta attctgtaag tgctgtgtga ccttctacca tttgagttaa
    24001 tgaagctgaa tatatagctc ttagttcagg tgtactactt gtcaatgcag aattaagata
    24061 catattggca gatgctttag cagatgaaag catacttaac attattactt tatcgtctat
    24121 ataagtattg ttcttaatta gattttctat catatttctc atgttatcta tacctcctca
    24181 gaaattagta tttcattttc aactataaat tgttgtatag ctttaattct tccttctgtt
    24241 gctaatatac tagattctga ttgtcttttt agatcttcat ctgatattaa catatttatt
    24301 gctctttgca taagtaatcc gtcagactcc atttttataa ttgctgctaa agatattttt
    24361 tcagtttctg aaagtattct catttaatca tctcctatat tgtaatttat gctttaatag
    24421 tatgtctttc ttttcaagga aataaccatt acactttttt acagtatttt atattctcgt
    24481 tatatcaaaa tttagtcgac ttaatatatt aatataattt tgtaggatat ttatcattaa
    24541 atatctataa aataaattgt ttgaatattc ataaagcaaa atttatttgt atgtacttct
    24601 tcaagagtaa atatgagttt tttcaaaaaa ttaattttta tgataattat taagggctat
    24661 atttttctta aagttgtgat tatcattatt aatttacaat aaaattgccg aaaagccctc
    24721 tcctttaggt gtagggatga aaggtaaggt cttaatataa aaaagttaga ataatgcttg
    24781 taatctaata ccgcta
//
LOCUS       DAMJVK010000053        24649 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.054, whole genome shotgun sequence.
ACCESSION   DAMJVK010000053 DAMJVK010000000
VERSION     DAMJVK010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24649
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.054"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     regulatory      129..219
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            628..945
                     /gene="flgB"
                     /locus_tag="PC414_002251"
     CDS             628..945
                     /gene="flgB"
                     /locus_tag="PC414_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HDF2464644.1"
                     /translation="MNTYDIIKMGLDATNLRSQTIANNIANINTEGYKRHYITFEETL
                     NDAMPKIEEKTDNSKGLRVDGNNVDIEEEKVNQAMATLQYNALISFANGKIAMTKSII
                     SGR"
     gene            953..1357
                     /gene="flgC"
                     /locus_tag="PC414_002252"
     CDS             953..1357
                     /gene="flgC"
                     /locus_tag="PC414_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HDF2464645.1"
                     /translation="MSVFSGMRISASGLTAESLKMDIISSNVANINATRGPNGEAYRR
                     KVVSFEENYDNKLGMLGVKVTSVEDDKSPLKEVYDPSHPDAKPNGYVEYPNVNILVEM
                     TDLITASRAYDSNVDTLNAQKSMISKALEIGK"
     gene            1367..1684
                     /gene="fliE"
                     /locus_tag="PC414_002253"
     CDS             1367..1684
                     /gene="fliE"
                     /locus_tag="PC414_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HDF2464646.1"
                     /translation="MPITQINNLMQGAKLDNLIGEAKPLTLNNKEEKKNFEDVIIDTI
                     DKVNFQQVTADDMKEKFIKGEDVSMHEVMLIGQEAQLSMQYLIEVRNKIHDAYQEMNK
                     VQI"
     gene            1709..3259
                     /gene="fliF"
                     /locus_tag="PC414_002254"
     CDS             1709..3259
                     /gene="fliF"
                     /locus_tag="PC414_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HDF2464647.1"
                     /translation="MITSFKNKIKEQVAKSRTKISSLKKGTKIALGIGVIAICLALVF
                     TAISASKNKYAVLFSELDAQDAADISAELESKKIDTKVKGSTIYVPRTEVDKLRLELA
                     PSLTGGSKGFALMDDSSSIGLTDEEFQIKKQRMIQGEIEKTIKSFPQVEDTRVHITFG
                     ESSVFEKEAVPGSAAVYLMLKPGTTLEKNQIKSVMALISGSVTNIPEKNVQVIDNKMN
                     LLSEGILEDENVDENNQPINSGISVDKNKASEQALNKQLEKSILEMLEPIFGKGKVKA
                     TVNADLSFDTAEKTEIKIDPDKVAIKETRSKNSSKSAANNVQGVDANMNNQGNNNGTN
                     TEDSLDESFEYETGKIETHTIVAPGELNRLTASVAIDGKIAKGVQADVEDIVNNAIGM
                     DGARGDTLSVVSMVFDPEGKKEAKQELEEIKKEGFKKNIIKAVIGVVAVIAVGAIGYL
                     IYKKRSEREDMDYEDEDYISKSDSVDRKDNPLLGDDPELTLEEAVKMYAEEKPDQVTE
                     IIKTWLNE"
     gene            3272..4342
                     /gene="fliG"
                     /locus_tag="PC414_002255"
     CDS             3272..4342
                     /gene="fliG"
                     /locus_tag="PC414_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HDF2464648.1"
                     /translation="MSTEITNVNAFDLSDINESDKNEKVEGEGLKKAAVLLMTLGPNV
                     SSDIIKSLPDKQVQKIGLEIANISSIASKERREILKEFVEINRAKDYVIEGGLEYARE
                     LLSNALGNVKANKLLEGISINTSTKPFKHIIKLDVKKILEILRNESAQTIAVLLAHME
                     PENGAEVLSYLDFDLQNEVAMRISSMGSISPEVIKVIESSLEDKLHSISGDDFNDYGG
                     VSTLVQILINSDRKTEKNILGTIEEQNPDLAAQIKSHIFVFEDIVKLDDISIQKVLKE
                     VTMKDLVYALKGVDDEISDAIYRNQSSRAADALKEEMEMVGVIKVTQVEASQQKIANI
                     VRKLEREGAITIVRNFGGDFVE"
     gene            4335..5066
                     /locus_tag="PC414_002256"
     CDS             4335..5066
                     /locus_tag="PC414_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HDF2464649.1"
                     /translation="MNNKIIKSKKALYKGVLDLTIIDKVRVGESNVNKENDCRFRDEV
                     IKEAIESAEIRKNEILEEAKMNAQNIEKQAYEKGYSQGQKNGYEDGYKESYDEYTQKA
                     KDEADFIKNQADLILLQANEKMTEYIKEKNKEIVRLAINMASSVLKKHFEDEESMSDL
                     LKQIIQDYEGSSSFIVRCNSFYKDNIEKEFELFKEETSLKSSVFVIADDSIDKGNAII
                     QKENGRIIVGIDCAMEKLKEELLGE"
     gene            5070..6389
                     /gene="fliI"
                     /locus_tag="PC414_002257"
     CDS             5070..6389
                     /gene="fliI"
                     /locus_tag="PC414_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HDF2464650.1"
                     /translation="MYKTDISIDFDKINKKIENLELTISEGRVKKIIGLTVEVEGIKA
                     FVGELCVIYNQVNKPVNCEVVGFKDKEVILMALGELTLIAPGCKVISKGIPLSVMCSD
                     NLLGKVLDGLGNPIDNSDAVLGDRYNLNNEPPDPMKRKKIRNIMETGVRAIDAFTTCG
                     EGQRIGIFAGSGVGKSTTLGMIARNAKADVNVIALIGERGREVLDFIDKDLGEEGMKK
                     SVVVCATSDKAPLVRLKGALTATAIAEYFRDQGKKVILMMDSVTRFAMAQREVGVAIG
                     EPPAQKGYTPSVFAMLPKLMERTGTSDKGSITAFYTVLVDGDDFNEPIADTTRGILDG
                     HIVLSRDLANKNHYPSIDVLNSLSRLMNEIASKEDIKIASFARDMLAEYREAEDLINI
                     GAYASGTNKKIDEAIYYHEHIINFLKQGINEKSSFNETISSLRRIFE"
     gene            6421..6867
                     /gene="fliJ"
                     /locus_tag="PC414_002258"
     CDS             6421..6867
                     /gene="fliJ"
                     /locus_tag="PC414_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012815984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HDF2464651.1"
                     /translation="MELDKNFKFRLQKVLDLKIKDEEEIKMEFAKIQQKKIDIETNLE
                     NLESNYSKYSISKNNDNVQNQKITINYLLALNNSIMDLSEELDKSTNELEKARKQLIS
                     KQIERKSLEKLKEKKYGQYYKEEQLKEQSTNDEFASMSYLRNRQVL"
     gene            6895..8100
                     /locus_tag="PC414_002259"
     CDS             6895..8100
                     /locus_tag="PC414_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HDF2464652.1"
                     /translation="MKIQADSYNIIKDIGNRKNSSKNKNIKNNKFEDNLYKTTDHKNV
                     KDRRETSKQNVSSDRPGNSDNECHLDEDKSNHLEDENTYEKDCSKNNEALQQLLNLLK
                     ENYKKDKDINTILNNIKKLSISDELKQELKLNKNILNKIANQNLELNGANTNNPNDIE
                     NLDIMKLRELLENNASEDIQVISKTTENDTKSIDLYSFDSNRMDTLNSNKQKDDSSDI
                     LEKLAGLNGNKSGNFGQVINKSVDMNKIKNDNVNLKQIESNVMDDSIKAIKHMKTNDI
                     QELTIKLRPKELGDMNIQLLKDGESMRAVVTVFNKDVFDSINKNIADLKQHLELTNVN
                     IKDVSVQMHSDNTNTSDTFDKAFEQQNRQNNQENMSNQNTNDKRNNVVIEDEIKENII
                     DDDRVDLLA"
     gene            8118..8726
                     /locus_tag="PC414_002260"
     CDS             8118..8726
                     /locus_tag="PC414_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgD"
                     /protein_id="HDF2464653.1"
                     /translation="MYGIATALAGTQVNYRSNNLSQLERNINDIANKQVRSKGNQTVT
                     DNGTPIIGQGDDADKDLFMKILVAQMGNQDPMNPQDPTEYVSQLAQFATLEKMSAMND
                     KLDILLALSNSTLINSALSMATSLIGKEVEVSGEEDEDENIVGVVKSTYIKDGEVHLE
                     LEVDGSDKTVDVKYEKLLKIGEVEANSGEENDSSEQESPEEV"
     gene            8753..9736
                     /locus_tag="PC414_002261"
     CDS             8753..9736
                     /locus_tag="PC414_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HDF2464654.1"
                     /translation="MIKAMYSGVSGMKANQTKLDVIGNNVANAGTTSFKKSSARITDS
                     FYQTVLYASAPTAALGGTNLGQVGVGSKISSINKDMTQGNVQPTNRPSDLMIDGDGYL
                     PVVRQGTVMYTRDGSFNLDTGFKAADGALKNIKGGRLVNGDGYMLQGMIYDGEYKADT
                     GEFVKKGDAKGKEPIVIPLQSISPKDGTVQNVLEYNISKDGTVEFLLSDGQRTQFVYD
                     IPDGAKVGDTPAGIDKLKGTIQKVQTYAFQNPAGLDAEGGNLFKPSANSGDPQIAGAT
                     GTIIQGAIEASNVDIAEEFTEMIIASRSFQANSKIISTSDEILQEIINLKR"
     gene            9749..9952
                     /locus_tag="PC414_002262"
     CDS             9749..9952
                     /locus_tag="PC414_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HDF2464655.1"
                     /translation="MIRVTDLNGRKYILNSDLIVRIDSVPESRILLTTGEKNLVKESV
                     DEIVDKVIKYKRKINVGYIRSEK"
     gene            9952..10770
                     /locus_tag="PC414_002263"
     CDS             9952..10770
                     /locus_tag="PC414_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HDF2464656.1"
                     /translation="MKKTDSLTFIGLILSTVLVLFGAVKGSSSGLKSFFDFASILITV
                     FGSFGALMITFTIDDIKLIKDALQYSFKTMNISKLDLLNQFKTLSKKARREGLLSIES
                     DVMKIQDPFMKKGLELCIDGLEINEIRSILELEIDSVEETNNRYSKLFKTWGTFAPAF
                     GMLGTLIGLIQMLSDLTSPDLIASGMGKALITTFYGSLLANIALNPIAYNIDEKTEKE
                     IYVKEMMLEGIISIQSGESSIVVEERLATYLSNNEKLEIMKSNKNTERAMSNGA"
     gene            10760..11455
                     /locus_tag="PC414_002264"
     CDS             10760..11455
                     /locus_tag="PC414_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDF2464657.1"
                     /translation="MARKKNKKKDEINPDAWLATYADTITLILTFFVLLYATSVVDME
                     KFKNIADALKGQFSGTSMFSTGSSTSEKSPIESVLVDKNDKSNLEQDLKDKVNNSNLQ
                     NSVTVKDDSRGILLELDDSILFDSGISELKEDSKATLNKVYDMIKMMKNKIVIEGHTD
                     NVPVKSSTHESNWDLSSSRAVSVVRYFVEEKGMDPKLFSATGCGEYQPLVSNETAKGR
                     AENRRVNILITID"
     gene            11467..11910
                     /locus_tag="PC414_002265"
     CDS             11467..11910
                     /locus_tag="PC414_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HDF2464658.1"
                     /translation="MFKNKKIIIIILILIIFSAGAGFFILGEGGNKQIDKIAGLFIPT
                     GKGKDEVKTENLDLEEMVLKLADEGVRYLKVSITVSYDSSYKDIEKNTPLIRDNIINC
                     FMAKRADDFTADNLKNIKEEIKNTLNIQLGENVIQNIYFTNIVVQ"
     gene            11925..12299
                     /locus_tag="PC414_002266"
     CDS             11925..12299
                     /locus_tag="PC414_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliZ"
                     /protein_id="HDF2464659.1"
                     /translation="MILYLLKILICIPIILILIVLSLKISQGTLLKKYNDKYIKILDI
                     VNISKNNSIIILKIGEEGCIMASSSSSMSKIKDLTKEEILRIENDIKERNFKIQDFKM
                     ERIKKKYDFSRYIGKFTKKEDK"
     gene            12300..12965
                     /gene="fliP"
                     /locus_tag="PC414_002267"
     CDS             12300..12965
                     /gene="fliP"
                     /locus_tag="PC414_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425215.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HDF2464660.1"
                     /translation="MELLSKLPLADVPYTSSMQLFLFLTALMFLPFVMFMMTSFVRIV
                     ISLSFLKSALGAQQAIPSQILVGLAIVLTIFIMRPVLNEINEKALQPYMKEEVTMEEA
                     MKEAEGPIKEFLLKQTRQTDLDLFVEQAGLKEKKLTRENIPLSVVVPAFAISELKTAF
                     QIGFLIYIPFLIIDLVVASVLMSMGMFMLPPVMISLPFKLLLFVMVDGWNLIVKTLIL
                     GFG"
     gene            12978..13247
                     /gene="fliQ"
                     /locus_tag="PC414_002268"
     CDS             12978..13247
                     /gene="fliQ"
                     /locus_tag="PC414_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HDF2464661.1"
                     /translation="MSEFMIMSVLKNGMFTGAMIAAPVLIVSLVVGLLISVFQATTQI
                     QEQTLTFVPKLIAILLVVAIGGSWMLHTLISFTSEIFNMMSLITK"
     gene            13259..15073
                     /locus_tag="PC414_002269"
     CDS             13259..15073
                     /locus_tag="PC414_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused FliR family export protein/FlhB family
                     type III secretion system protein"
                     /protein_id="HDF2464662.1"
                     /translation="MIFVFIRIVAFFGTLRILFPAGTPALFKSLFAIIISILISSTMQ
                     IEYATNIDNIVLFTLYGINETITGIMLGYITNLCFYSIRMAGSMMDQQMGLSMINMFD
                     PNSMTQTTLINNLMNWTALMIFFSMDGHHVLIRGIRYSFELIPIGKSFIDNNIDYIIN
                     IFVQCFLTGFKIAIPIVLCLLMADFILGLISRSVPQLNVMIIGMPLKILVGIALFIIS
                     IPLIVNQISHLLSQIPKMYEGTFALAPMFFMGSTDKTEEATPKKKGEQRKKGNIAKSK
                     ELPIAMTLLAFTLLVPTLFSYVVNTLKSSLNYFLSLDFYMNINYSSIEKLVIAGLMDF
                     FKIFLPIAIPFLVLGIVANLFQVGILFTGETLKPNLSKLNPISGFKNMFSMRSLSTLI
                     KDTAIISILAYIGYTFFQDNYLDILKLGNIYLPTLMYTVKDLVYSILSKICVAMIVIA
                     VADYVYQRYSHKKQLRMTKQEVKDEYKNSEGDPEIKAKIKQKQRQISSQRTMQAVPSA
                     TVIVTNPTHLSIAVRYEKGKDQAPVVVAKGADYLAFKIREIAKGNDIPIIENKPIARL
                     LYKQVEIDQEIPEDMYQAFAEILVAVYKIKNRYKVPKR"
     gene            15082..17157
                     /gene="flhA"
                     /locus_tag="PC414_002270"
     CDS             15082..17157
                     /gene="flhA"
                     /locus_tag="PC414_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HDF2464663.1"
                     /translation="MNKFNLKENMDVIVAFGVVGIVLMMIVPLPTFILDILLAINISL
                     SVLILLMTLFTTNVLDLSIFPTVLLVTTLFRLGLNLSSTRLILTQGYAGEVIEAFGSF
                     VVGGNYVVGIIIFLIILIVQFVVITNGSGRVSEVTARFTLDAMPGKQMSIDADLNAGV
                     IDDKTARKRRKELQQEADFYGSMDGANKFVKGDAIAGLIVTAINIIGGIVIGAVMLNM
                     TLMGAAQTYVRLTIGDGLVSQIPALLISTSAGIIVTRASTDENFGGLVGKQLTAIPKA
                     VMMTGCVLIVLGMMPGLPKPAFFSLGIGAIAAGYFLGREKKDTLEDLEFEKPDMVNPD
                     KNEVEDVTNLINVESIEVEIGYGLISLADESAGGDLLQRIASIRRQCAIDMGIVVQPI
                     RIRDNLQLNPNQYNIKIKGNVVGGYEIMPNMVLCMDPMNQGFSIFGIKTIEPTFGIEA
                     LWIESDKVEDAELKGYTVVDSSTILITHLLDIIKSNAHELLGRQEVKTIIDAARENYS
                     AVVDELIPDLMTLGEIQKVLKNLLREKVNIKDRVTILETLADQSRNTKDIELLTEYVR
                     IALARSICNNLVDEDKAIVVATLSPETEELVSNNLQRSVNGTYPAVDPENTNKIFESI
                     QEVMNNVYFNHNIPVIVVSPKIRAPFRKLVEIVYPNLTVLSLNEIPNDIKIKAEGVVS
                     IYDDEKVYS"
     gene            17132..18148
                     /gene="flhF"
                     /locus_tag="PC414_002271"
     CDS             17132..18148
                     /gene="flhF"
                     /locus_tag="PC414_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HDF2464664.1"
                     /translation="MMTKKYTANTIQDAMNLAKMELGDNITLIDKKEVRKSGIRGIFS
                     KKDIELTIGWEKKDNLEQKDLKREIEQLKSIINNMGFDNKNDNDIDKICKNLLNLDLN
                     QEIVEFIKADLQEMKFNGIDTSKNLVESLKKKIKIENQAINGKIALVGPPGVGKTTTI
                     AKLAAKLVFEENKKVGVITIDTYRIGAVEQLKIYTDIMNIPFKGVISPDEMELALDEM
                     KDCDVVLIDTTGRGYKNSMQILEIKNLIDKAETDNIHLVLNCTTRESDTKAIIDSYRN
                     VNFKSLIITKLDETITYGSIFNIMNYAQKPISYITTGQNVPDDIIKPNEDKIIRLLLG
                     VESI"
     gene            18145..19023
                     /locus_tag="PC414_002272"
     CDS             18145..19023
                     /locus_tag="PC414_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HDF2464665.1"
                     /translation="MIDQAQILRKMAMEKRGSDEIIVENENMPKIITIASGKGGVGKS
                     NLATNLSICLTKLDKKVLILDADIGMSNIDIIMGVNVKGTIIDVINGEKNIEDIISQT
                     KYGVNIISGGSALNHIEDFTEAQRNKFIHSIEQIHNVDFIIIDTGAGMSKSLLSFIYC
                     STEFFLITTPEPTSLTDAYSLLKAISNFGIKKSANIIINRVIDLEEAKSTYKRIKMVV
                     DKFLNLNLELYGYIIDDKKVGVCVKKQIPFILEYPTSIASKCIIAIAKRMVSQSREDI
                     VDNKSFLRKMFSIFKS"
     gene            19037..19738
                     /locus_tag="PC414_002273"
     CDS             19037..19738
                     /locus_tag="PC414_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HDF2464666.1"
                     /translation="MNREELIKENMPLVKSIARKFFIPGKGFEYQDLVNSGVIGLIDA
                     INKFDTEKGAKFSSYSYIKIKSAILDEIRNQSPISKHNLTKVNKYNRVVEKLQSKLLR
                     EPTSYEIAKELKVSEKELHDIESNIDMLNIVSLNYVIFEDTNETVQDVISDREEEAPE
                     NIIEEEEKLEILSKAISNLNEREKLILSLYYYEDLNLKEIGKVLGVSESRVSQLHRKS
                     IRNLRNKIKELKYSI"
     gene            19754..20176
                     /locus_tag="PC414_002274"
     CDS             19754..20176
                     /locus_tag="PC414_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HDF2464667.1"
                     /translation="MIQIILIVVSILIIVTIVINLQKESKNKDNRYFDKIFEQEYEAN
                     DRLIIAEKRRFFLDKVLAEKRINSHIKVYENYNLIKANEERKENIFIESKKEVRQENE
                     LSLKVKHLISSGYDDVEICKMLDIGKGELLLIRSCYKI"
     gene            20161..20346
                     /locus_tag="PC414_002275"
     CDS             20161..20346
                     /locus_tag="PC414_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HDF2464668.1"
                     /translation="MLQNIRILLCDKKVFLGIGIGILISTLCIMLFSNNDMSKAEIEK
                     KARALGMKYTSEMKVLE"
     gene            20387..21160
                     /gene="flgG"
                     /locus_tag="PC414_002276"
     CDS             20387..21160
                     /gene="flgG"
                     /locus_tag="PC414_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HDF2464669.1"
                     /translation="MIRGLYTAVSAMITNQQKQNVVVNNLANMDTNGYKSKQLLTKSF
                     DELKLEGYNNYANGTKQKQIIGDISPGISMDETITNFNQGPIKTTDNKMDVAIQGKGF
                     FLVSDAAGNQFYTRNGNFRTDNQGDLITSEGYYVLGTNTATGAVGHINVGNQKFEVAK
                     DNTISLDGNAMYKFNIVDFNNYNNLKKEGDNLYSGGGAMKVNNALTIQSAIESSNVDM
                     VSEINNMMTISREYEANQKIIQAMDSKLAKIASEIGSVR"
     gene            21181..21942
                     /gene="flgG"
                     /locus_tag="PC414_002277"
     CDS             21181..21942
                     /gene="flgG"
                     /locus_tag="PC414_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HDF2464670.1"
                     /translation="MYTMFYTSKTALSANQSKLDIISNNIANSDSTGYKKINMEYSDL
                     VDEVLNRPSYPTNGKDISTGTGAKATAPMRNYEQGALAPTDSKSNLAIDGEGFFRVIR
                     KDGTYAYTRNGGFNVDALGKIVDDNGNILDVQFDAGYNYNNTNIDSTNLTVSRNGELF
                     VDNKKIGKINLYQPIGTQNFISEGDSLFVATDAAQIKQVEKVDIVQGYRERSNVSLQE
                     EFVDLIATQRAFQMNSKGIKTADEMWQIANNLRAK"
     gene            21965..22846
                     /locus_tag="PC414_002278"
     CDS             21965..22846
                     /locus_tag="PC414_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HDF2464671.1"
                     /translation="MNDMKEYDFGKPQSYSRENLTCLNFLLAEFCKKYKNYIIYELKC
                     NSSMVVDSIDQVNYQTFLDRVTSSCVVVQNAMEPVMKNFSFRIDRSVADMWIDITSGG
                     TGVVKDNDRELTELDKKVLLHLMDDLVRNMHLFEGFENIQVLDTHTHINLPQLCSPTA
                     PVCVVDLKVLNDNECIGSVSLCFPYNGLEALLESISVIEFFDNDVGDDSEEFTQKIYN
                     SVSNIELSVIAEIGKVSINVEDLLKLEVGDVIVTNKKINDYIDIFVENSKSYTATPGF
                     ISSKKGVKINDAVGKEV"
     gene            22849..23223
                     /locus_tag="PC414_002279"
     CDS             22849..23223
                     /locus_tag="PC414_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HDF2464672.1"
                     /translation="MDNSNLISDEEAKMLLNDKVESEVDGDDELALDVSEVNRNNIQR
                     ILDLKLELSVVIGRTKMTLRDILNLHKGSLIELDTLADQDVEILIDNKVLAYGKVVVV
                     DLNFGVKITSIVSEEDMVKSLV"
     gene            23255..>24649
                     /locus_tag="PC414_002280"
     CDS             23255..>24649
                     /locus_tag="PC414_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDF2464673.1"
                     /translation="MVLILNLNDIKKDILKLNKKDSCSLYNESINNINNNCISTGLKF
                     LKQARDLNPDDVDILNLTGLVNLLKCNFDDAVESFYKSSLCEKTALCEKYINMLTSEE
                     FLIFFEKYNQLIRLINKGMYKEAIQGFKLITDQYCDLIEPYELLALLYDKEEEYIKFD
                     ECLEVLKIIDKENYLLNQENNTLDMYNSANIQTKSNIKVDDYKNEFLHKSSSNHKIND
                     YNDVTKEKSSSKNQKSSDSNKKKSIKKNKYLYIAIILGILLVGQSIYNSYKMNNLTDK
                     ISKNNETKNEESIDSNLTKKENNIEENEDMSKASNINKSNKNINKEDDVKSKKNTVEN
                     EDDKDSDLFTEDELMSKANTLKSEKKTKSSIQLYKKVADIGENKGNTSEATYQVAILS
                     EKLKDYKTAEEYYKMYVENYSEKDAYFDESYYNLGMMYYNNGDLKNSKLTLKKLVNKV
                     PNSMYNNSKVKEILK"
BASE COUNT        10216 a         2634 c         4585 g         7214 t
ORIGIN      
        1 tataaaattg taaactatag tgtttataat ctttgtaatt attggtaatt aaatttaaaa
       61 aataaaatat ttaattatac acaaaaaaga tatttaaaaa atttgttatt tattgtatac
      121 ttaagttaaa actaaatagg caaatctaga gaaatctagt gacgcaaagc tatagggact
      181 aaggtttata tacataaact atgtcagcca gttgccaaaa agagtcctag gtgtattgta
      241 tacctaagaa aagtctataa tgactgcctt tttggcagtc attttgttat ggaaaaatca
      301 ctttatttca gtagaaaata cacataataa catattttac caattataac ataaaataaa
      361 gaaaaattat ctcaaaaagt tactatttta caaaaaagag caacttttcg aagaaatatt
      421 taaatacata ttaaaatttt tttatatttt catgggactt cattaaaaaa atattagttt
      481 tcttactaaa ttgatacatt attgtattaa tgtaatggaa atgcgattaa tttaaataaa
      541 taaagaagtc attttttgta atatagcaac ttcatttttt ttattaataa acaaaataga
      601 taaaatatat tttgaggagg ggtgagtgtg aatacatatg acattataaa aatgggatta
      661 gatgcaacta atctaagaag tcagacaata gcaaataata tagcaaatat aaatactgaa
      721 ggatataaaa gacattatat aacttttgaa gaaacactaa atgatgctat gcctaaaata
      781 gaagaaaaaa cagacaattc taaaggttta agagtagatg gtaataatgt agacatagaa
      841 gaagaaaaag taaatcaagc aatggcaaca ttacaatata atgctttgat aagttttgca
      901 aatggaaaaa tagcaatgac caaatcaata ataagtggga ggtaattaga agatgagtgt
      961 gttcagtgga atgagaatat ctgctagtgg attaacagca gaaagtttga aaatggatat
     1021 catatcatcc aatgtagcta atataaatgc tacaagaggt ccaaatggag aagcatatag
     1081 gagaaaagtt gtttcatttg aagaaaacta tgataataaa ttaggtatgc taggagtaaa
     1141 agttacatct gttgaagatg ataaatcacc tcttaaagaa gtatatgacc catctcatcc
     1201 agatgcaaag ccaaatggat atgtagaata tccaaatgta aatatattgg tagaaatgac
     1261 agatttaata acagcttcac gagcatatga ttctaacgta gatacattga atgctcagaa
     1321 gtctatgata tcaaaagctt tggaaatagg aaagtaggga taaattatgc caattacaca
     1381 aattaacaat ttaatgcaag gtgcaaagtt agataatttg attggagaag caaagccatt
     1441 aactcttaat aataaagaag aaaaaaagaa ttttgaagat gtaataatag atactataga
     1501 taaagttaat tttcaacaag taacagcaga tgatatgaaa gaaaaattta taaaaggcga
     1561 agatgtttct atgcatgaag ttatgttaat aggtcaagaa gcacaactat ctatgcaata
     1621 tttaatagaa gttagaaata agattcatga tgcatatcaa gaaatgaata aggtgcaaat
     1681 ttagcctttt gaacaggagt aataatcaat gataaccagt tttaaaaata agattaagga
     1741 gcaagttgct aaatctagga caaagataag ttctttaaaa aagggaacta aaatagcatt
     1801 aggtataggg gttattgcta tatgtttagc gctagtattt acagcaatat ctgcgagcaa
     1861 aaataagtat gcagtcttat tttcagaact agatgctcaa gatgctgcag atatatcagc
     1921 agagctagag tctaaaaaga tagatactaa ggtaaaagga agtacgattt atgtacctag
     1981 aacagaagtt gataagttaa ggctggagtt agcgccaagt cttacaggtg gttctaaagg
     2041 atttgcacta atggatgata gttcatcaat agggcttact gatgaagaat ttcagattaa
     2101 gaaacaaaga atgatacaag gtgaaataga aaaaacaata aagagttttc cacaagtgga
     2161 agacactaga gtacatataa catttggaga gtcatcagta tttgagaagg aggctgttcc
     2221 agggagtgca gcagtttatt tgatgctaaa accagggacg actttagaaa aaaaccaaat
     2281 aaagtctgtt atggcactta tatcaggaag cgttacaaat ataccagaga aaaatgttca
     2341 ggttattgat aacaaaatga atttattatc tgaaggtatt ttggaggatg aaaatgttga
     2401 tgaaaataat caaccaataa atagtggcat tagtgttgat aaaaataagg catctgaaca
     2461 agcattgaat aaacaactag aaaagtctat tttagaaatg ctagaaccga tatttggtaa
     2521 gggaaaggtg aaagctacag taaatgctga tttaagtttt gatacagctg aaaaaactga
     2581 gataaaaatt gaccctgata aagttgcaat aaaggagaca aggtcgaaaa attcatctaa
     2641 atctgctgca aataatgttc aaggtgtaga tgctaacatg aataaccaag gaaataacaa
     2701 tggtactaat acagaagata gtttagatga gagttttgag tatgaaacag gtaagattga
     2761 aactcacact atagttgcgc ctggagaact taatagattg acagcttctg ttgcaataga
     2821 tggaaaaata gctaaaggtg ttcaagctga tgtagaggat attgttaata atgctatagg
     2881 tatggatgga gcgaggggag atactcttag cgttgtttca atggtatttg acccagaagg
     2941 taagaaagag gctaaacaag agctggaaga gattaaaaaa gaaggattta aaaagaacat
     3001 aataaaagca gttattggag tagtagcagt aatagcagtt ggagctattg gatacttaat
     3061 atataagaaa agaagtgaac gagaagatat ggattatgaa gatgaagatt atatttctaa
     3121 atctgattct gttgatagga aggataatcc tctattagga gatgacccag aactgacatt
     3181 agaggaagct gttaaaatgt atgcagaaga aaagccagac caagtaacag agataataaa
     3241 gacttggtta aatgagtaag ggggatagaa agtgtccacg gaaattacaa atgttaatgc
     3301 gtttgactta agtgatataa atgaaagtga taagaatgag aaggtagaag gtgagggttt
     3361 aaaaaaagct gctgtattat taatgacatt aggcccaaat gtgtctagtg atataataaa
     3421 aagcttgcct gataaacaag ttcaaaaaat aggtttggaa attgcaaata tttcttcaat
     3481 agcttcaaaa gaaagaagag aaatcttaaa agagtttgta gaaataaata gggcaaaaga
     3541 ttatgttata gaaggtgggc ttgaatatgc aagagagtta ttaagtaatg ctttaggaaa
     3601 tgttaaagca aacaaattac ttgaaggtat atcaataaat acaagtacaa agccttttaa
     3661 acatattata aaactagatg taaagaagat attagaaata cttaggaatg aaagtgcaca
     3721 aactatagcc gttttacttg cacatatgga accagaaaat ggagcagaag ttttatcata
     3781 cttagatttt gatttacaaa atgaagtagc tatgagaata agctcaatgg gttcaatatc
     3841 tccagaagta ataaaggtaa ttgaaagttc attagaagat aaattacatt caataagtgg
     3901 tgatgatttt aatgattatg gaggggtgtc taccttagtt caaatattga ttaattctga
     3961 tagaaaaaca gaaaagaata ttttgggaac tattgaagaa caaaacccgg atttagcagc
     4021 tcaaattaaa tctcatatat ttgtctttga agatattgtc aagttagatg atataagcat
     4081 acaaaaagtg ttaaaagaag tgactatgaa agatttagta tatgccttaa aaggtgtaga
     4141 tgatgagatt tcagatgcta tatatagaaa tcaatcttct agagcagcag atgcacttaa
     4201 agaagaaatg gaaatggttg gtgttataaa ggttacacaa gttgaggctt ctcaacaaaa
     4261 gatagctaat atcgtaagaa aacttgaaag agaaggtgcc ataactattg taaggaattt
     4321 tggtggtgac tttgttgaat aataagatta ttaaaagtaa aaaagcatta tataaaggag
     4381 tgctagattt aaccataata gataaagtta gagtgggaga atcaaatgta aataaagaaa
     4441 atgattgtag atttagggat gaggtaataa aagaagctat agaatctgct gagataagaa
     4501 agaatgagat attagaagaa gcaaaaatga atgcacaaaa tatagaaaag caagcatatg
     4561 aaaaagggta tagtcaaggt caaaaaaatg gatatgaaga tggatataaa gagtcatatg
     4621 atgaatatac ccaaaaagct aaagatgaag cagattttat aaaaaatcag gcagacttaa
     4681 tactattaca agctaatgag aagatgactg agtacataaa agaaaaaaat aaagagattg
     4741 taagacttgc tattaatatg gctagttctg ttctaaaaaa gcatttcgaa gatgaagaat
     4801 ctatgagtga tttgttaaag caaattattc aagattatga aggaagtagt tcatttatag
     4861 taagatgtaa ttctttctat aaagataata tagagaaaga gtttgaatta ttcaaagagg
     4921 aaacatcact aaaatcaagt gtatttgtta ttgcagatga tagtatagat aaaggcaatg
     4981 ctattatcca aaaggaaaat ggaagaataa tagttggtat tgattgtgct atggaaaaac
     5041 taaaagaaga attgcttggt gaataaagta tgtataaaac tgatatatct atagattttg
     5101 acaaaataaa taagaaaata gaaaatttag aactcactat atcagaaggt agagtaaaga
     5161 agataatagg acttactgta gaagttgaag gaataaaagc ttttgttggg gagttatgcg
     5221 ttatatacaa ccaagttaat aaaccagtga attgtgaagt agtaggtttt aaagataaag
     5281 aagtcatatt aatggcgttg ggagaattaa cattgatagc acctggttgt aaagttattt
     5341 ctaaggggat acctttaagt gttatgtgta gtgataattt actaggtaaa gtacttgatg
     5401 gtcttggaaa tcctatagac aactcagatg ctgttttagg agatagatac aacctgaaca
     5461 atgaacctcc tgacccaatg aagagaaaaa agatacgaaa tataatggaa acaggtgtaa
     5521 gagcaataga tgcatttact acttgtggtg aaggacagag aatcggcata tttgcaggaa
     5581 gtggagtagg taagagtacg actctaggta tgattgctag gaatgcaaaa gcagatgtaa
     5641 atgtaattgc tcttattgga gaaagaggaa gagaagtact agattttatt gataaagatt
     5701 taggtgaaga gggaatgaaa aaatctgttg ttgtgtgtgc aacatcagat aaagcccctc
     5761 tagttaggtt aaaaggtgct cttactgcta ctgcaatagc agaatatttt agagaccaag
     5821 gcaaaaaagt aatacttatg atggattcag taactagatt tgctatggca caaagagaag
     5881 ttggtgtagc aataggagag ccaccagctc aaaaaggata tacaccatct gtatttgcaa
     5941 tgttgccaaa acttatggag agaacaggga catcagataa aggttctata actgcatttt
     6001 atacagtatt agttgatgga gatgacttta atgagccaat agcagataca acaagaggga
     6061 tacttgatgg tcatatagtt ttatctagag acttggcaaa taagaatcac tatccttcta
     6121 tagatgtatt aaacagttta agtaggctta tgaatgaaat tgcttcaaaa gaagacataa
     6181 aaatagcgtc ttttgcaaga gacatgcttg ctgagtatag agaagcagaa gatttgataa
     6241 atataggtgc atatgcttca ggtacaaaca aaaaaataga tgaagcaatc tattatcatg
     6301 agcatattat taatttttta aaacaaggaa taaatgaaaa gagttctttt aatgaaacta
     6361 taagtagttt aagaagaatc tttgaataaa caatttaaaa aaatatttga ataggttgtg
     6421 atggaattgg ataaaaattt taagtttaga ttacagaaag ttttagactt aaaaataaaa
     6481 gatgaagaag agataaaaat ggaatttgct aagattcaac aaaaaaaaat agatatagaa
     6541 actaatttag aaaacttaga aagtaattac agcaaatatt ctataagtaa aaataatgat
     6601 aatgttcaaa atcaaaaaat aactattaac tatttgctag ctttaaacaa ctctataatg
     6661 gatttatcag aagaactgga taagtcgaca aatgaacttg aaaaagctag aaaacagctt
     6721 ataagcaaac agatagagag aaagtcatta gaaaaattaa aagaaaaaaa atatggacag
     6781 tattataaag aagaacagtt aaaagaacaa agcactaatg atgagtttgc aagcatgagt
     6841 tatttgagaa atagacaagt tctataaaat aataggaagt gaggatatta ttgtatgaag
     6901 atacaagcag attcttataa tattattaaa gatattggga atagaaaaaa ctcatctaag
     6961 aataaaaata taaaaaataa taaatttgaa gataatctat ataagacaac agaccataaa
     7021 aatgttaaag atagaagaga aacaagtaag caaaatgtta gttcagatag accgggaaat
     7081 agtgataatg agtgtcattt agatgaagat aaaagtaatc acttagaaga tgaaaataca
     7141 tatgaaaaag attgtagtaa gaataatgaa gctttacagc aattactgaa tcttttaaaa
     7201 gagaattata aaaaagataa agatataaac actatattga ataatataaa aaaactaagt
     7261 atatcagatg aattaaaaca agaacttaag cttaataaaa atatattgaa taaaattgca
     7321 aatcaaaatt tagagttaaa tggagctaat acaaataacc ctaatgatat agaaaattta
     7381 gacataatga agttaagaga acttttagaa aataatgcta gtgaagatat tcaagtaatt
     7441 agtaaaacta cagaaaatga tactaaaagc atagacttat acagttttga tagtaataga
     7501 atggatactc ttaactcaaa taaacagaaa gatgatagtt ctgatatttt ggaaaaacta
     7561 gcagggttaa atggaaataa gagtggaaat tttggacaag taataaataa aagcgttgat
     7621 atgaataaaa ttaaaaatga taatgttaat ttaaaacaga tagaatctaa tgtaatggat
     7681 gattctataa aagctattaa gcatatgaaa acaaatgata tacaagaatt gacaattaaa
     7741 ttaagaccaa aagaattagg agatatgaac atacaattat taaaagatgg tgaaagtatg
     7801 agagctgttg tgactgtttt caataaagat gtctttgact ctataaataa aaacatagca
     7861 gatttaaagc aacatcttga acttacaaat gtaaatataa aagatgtatc agtgcaaatg
     7921 catagcgata atacaaatac atctgatacc tttgataaag catttgaaca acagaataga
     7981 caaaataacc aagaaaacat gagtaatcaa aatacaaatg ataagcgtaa caatgtagtc
     8041 atagaagatg agattaaaga aaatattatt gatgatgata gagtagattt actagcttag
     8101 taagggggaa gtattgcatg tacggaatag caacggcttt ggcaggaact caggtaaact
     8161 ataggtcaaa taacttaagc caacttgaaa gaaatattaa tgatattgcc aacaaacaag
     8221 ttagaagtaa aggaaatcag acagtaacag acaatgggac tccaattata ggacaaggtg
     8281 atgatgctga taaagacttg tttatgaaga ttcttgtagc acagatgggt aatcaagacc
     8341 caatgaatcc acaagaccca acggagtatg tatcacaatt agcacagttt gcaactttag
     8401 aaaaaatgtc agctatgaat gataagctag atatactttt agccttaagt aactctactc
     8461 taataaactc tgctttgagt atggcaacat cattaattgg taaagaagta gaagtatctg
     8521 gggaagaaga tgaagatgaa aacattgtag gagtagtaaa gtccacatac ataaaagatg
     8581 gggaagtaca cttagaatta gaagtagatg gaagtgacaa aactgtagat gttaaatatg
     8641 aaaaattact taaaatagga gaggtagagg caaattcagg agaagaaaat gactcttcag
     8701 aacaagaatc tccagaagaa gtataatatt aaatgggaaa agaggtaaat ttatgataaa
     8761 agcaatgtat tcaggtgtaa gtggaatgaa agcaaatcaa acaaaactag atgtaatcgg
     8821 taacaatgta gccaacgctg gaactacttc ttttaagaaa agttcagcta gaataacaga
     8881 ttcattttat caaactgtac tatatgcaag tgctccaaca gcggcattag ggggaacaaa
     8941 cttaggacaa gtaggtgtag gtagtaagat atcaagtata aacaaagata tgacacaagg
     9001 aaatgtacaa ccaactaata gaccatctga tttaatgata gatggagatg gatatcttcc
     9061 agtagtaaga caaggaacag ttatgtatac gagagatggt tcttttaatt tagatacagg
     9121 atttaaagca gctgatggtg ctttgaagaa tattaaaggt ggaagacttg taaatggtga
     9181 tggatatatg ttacaaggta tgatttatga tggggaatat aaagctgata ctggagaatt
     9241 tgttaaaaag ggtgatgcta aaggaaaaga gcctattgta ataccacttc aaagtataag
     9301 tcctaaggat ggaacagttc aaaatgtact agagtataat atatcaaagg atggaacagt
     9361 agaattttta ttgagtgatg gtcaaagaac tcaatttgtc tatgatatac ctgatggtgc
     9421 taaggtagga gatacacctg ctggaataga taaattaaaa ggtacaatac aaaaagtgca
     9481 aacatatgca ttccaaaatc cagcaggact agatgcagaa ggaggaaact tgttcaagcc
     9541 atcagcaaac tcaggagacc cacaaatagc aggagctaca ggcacaataa tacaaggagc
     9601 tatagaagct tccaatgtag atatagcaga agaatttaca gaaatgataa tagcaagtag
     9661 gtctttccaa gcaaacagta aaataatatc gacatcagac gaaatacttc aagaaataat
     9721 aaatcttaag agatagtagg tacatattat gataagagta acggatttaa acggaagaaa
     9781 atacatatta aattcagatc ttatagtgag aattgactcc gtgccagaaa gcaggattct
     9841 ccttacaaca ggagaaaaaa atcttgtaaa agagtcagta gatgagatag tagataaagt
     9901 aataaagtat aaaagaaaaa taaatgtagg ttatattagg agtgaaaaat aatgaaaaaa
     9961 acagattcac taacattcat tggactaata ttatctacag tattagtttt atttggagca
    10021 gtaaaaggaa gtagttcagg attgaaaagt tttttcgatt ttgcatctat attaatcaca
    10081 gtatttgggt catttggagc tttgatgatt acatttacta ttgacgatat aaaacttata
    10141 aaagatgctc tacaatactc attcaagaca atgaatatat cgaaattaga cttattaaat
    10201 caatttaaaa cactttctaa aaaagcaaga agagaaggac ttttatctat agaaagtgat
    10261 gtaatgaaaa tacaagaccc atttatgaaa aaaggattag agttatgtat tgatggtctt
    10321 gaaataaatg aaataaggtc tatactagag ctcgaaatag attctgttga agaaaccaat
    10381 aacagatatt ctaaactatt taaaacttgg gggactttcg cacctgcatt tggtatgttg
    10441 ggaactctaa taggtttaat acaaatgtta tccgacctta catctccaga tttaatagca
    10501 tctggtatgg gaaaagcatt aattacgaca ttttatgggt ctttacttgc caatatcgct
    10561 ttgaacccaa ttgcatataa tatagatgag aagactgaaa aagaaattta tgtaaaggaa
    10621 atgatgctgg aggggataat aagcatacaa tctggagaga gcagcatagt agttgaggaa
    10681 cgtttagcta catacctttc aaataatgag aagttagaaa ttatgaaatc caataaaaat
    10741 acagaaagag caatgagtaa tggcgcgtaa aaaaaataag aaaaaggatg agataaatcc
    10801 agatgcatgg ttggctactt atgcagatac gataacactg attcttacat tttttgtgtt
    10861 attgtatgct acatcagttg tagatatgga aaaatttaaa aatatagcag atgcgttaaa
    10921 aggtcagttt tctgggacaa gtatgtttag tacaggttca agtacaagtg agaaatctcc
    10981 aatagagagt gttttagtgg ataaaaatga taagtcaaat ttagaacagg atttaaaaga
    11041 taaagttaat aattctaact tacaaaatag tgtaacagtc aaagatgata gtagagggat
    11101 tttactagag ctagatgatt caattttatt tgactctggg atttctgaat taaaagaaga
    11161 tagtaaagcc actcttaata aagtatacga tatgataaag atgatgaaaa ataagatagt
    11221 tatagaaggc catactgata atgtacctgt aaaatcaagt acgcatgaat ccaattggga
    11281 tttatcttct agtagagcag taagtgttgt aagatacttt gtagaagaaa agggcatgga
    11341 ccctaaacta ttttcggcaa caggatgtgg agagtaccaa cctttggtat caaatgaaac
    11401 agcaaaggga agagctgaga atagaagagt taatatactt ataactattg actaggagga
    11461 agaggtttgt tcaaaaataa aaaaattatt ataattattt tgatactgat tattttctca
    11521 gcaggagcag gtttttttat attaggagaa ggtggaaata aacaaattga caagattgca
    11581 ggtttgttta ttccaacagg gaaaggcaaa gatgaagtaa agaccgaaaa tcttgactta
    11641 gaagagatgg tattgaagct agctgatgaa ggtgtaagat atttaaaagt atcaataact
    11701 gtatcttatg attcttcata taaagatata gaaaaaaata cgcctttaat tagggataat
    11761 attataaatt gttttatggc aaaaagggca gatgatttta ctgctgataa tttaaagaac
    11821 ataaaagaag aaattaaaaa tacattaaat atacaactag gtgaaaatgt aattcaaaat
    11881 atatatttca caaatatagt agtgcaataa aaggatggta agacatgata ctttatttac
    11941 taaaaatatt aatatgtatc ccaataattt taatattgat agtcttatct ttaaaaatta
    12001 gccaaggaac tttattaaaa aaatataatg ataaatatat aaaaatacta gatattgtaa
    12061 atataagtaa aaacaatagc ataataattt taaaaatagg tgaagaagga tgcattatgg
    12121 catcttcttc ttctagcatg agtaaaataa aagatttgac caaagaagaa atattaagaa
    12181 tagaaaacga cattaaagaa agaaatttta aaattcaaga ttttaaaatg gaaagaatta
    12241 aaaagaaata tgatttcagt agatatattg gaaaatttac aaaaaaggaa gataagtaaa
    12301 tggaactatt atcaaaatta cctttggctg atgtaccata tacaagttca atgcaattgt
    12361 ttttatttct aactgcatta atgtttttac catttgtaat gttcatgatg acaagctttg
    12421 tcagaatagt tataagttta tcatttttga aatcagcatt aggagcacag caagcaattc
    12481 caagtcaaat tttggtaggg cttgcaatag tattgacaat atttataatg agaccagtat
    12541 taaatgagat aaacgaaaag gcattacaac catatatgaa agaagaagtt actatggaag
    12601 aagctatgaa ggaagcagaa ggacctatta aagaattttt attaaagcaa accagacaga
    12661 cagatttaga tttatttgta gagcaagctg gtttaaaaga aaagaaatta actcgtgaaa
    12721 atataccatt atctgttgta gttccagcat ttgctataag tgaactaaaa actgcttttc
    12781 aaataggttt tttaatatat ataccatttt tgataataga ccttgttgtg gcaagtgttt
    12841 tgatgtctat gggtatgttt atgttacctc cagttatgat atcattacca tttaaattat
    12901 tattatttgt aatggtggat ggatggaact tgatagtaaa aacattgata ttgggatttg
    12961 gataggagaa aaatagtatg agtgaattta tgataatgag tgttcttaaa aatggcatgt
    13021 ttacaggagc tatgattgca gctccagtgc taattgtttc tcttgtagtg gggcttttaa
    13081 taagtgtatt tcaagccaca actcaaatcc aagagcaaac tttgactttt gtaccaaaac
    13141 taatagcaat tcttttagta gtagctattg gtggaagctg gatgttacat actcttatta
    13201 gctttacttc ggagatattc aatatgatgt ctctcataac taaataagta ggatatatat
    13261 gatttttgta tttattagaa tagtagcatt ctttggaaca ttaaggatac tatttccagc
    13321 tggtacacct gctttattta agtcgctctt tgcaatcatt atatcaatct tgatttcaag
    13381 tactatgcaa attgaatatg caacaaatat agacaacatt gtattgttta ccttatatgg
    13441 tataaatgag accataactg ggattatgct tggatatata accaatctat gtttttatag
    13501 tatcagaatg gctggttcta tgatggacca acagatggga ttatcaatga ttaatatgtt
    13561 tgacccaaat tcaatgactc agacaacttt aatcaataat cttatgaact ggacagcact
    13621 tatgatattt tttagcatgg atggtcatca tgttttaatt agaggcataa ggtatagttt
    13681 tgagttaatt ccaataggga aatcattcat agataataat atagattata taataaacat
    13741 atttgtgcag tgctttttaa ctggattcaa aatagcaata cctattgtac tttgtctgct
    13801 tatggcagat tttatattag ggcttatatc aagaagtgta cctcaactaa atgtaatgat
    13861 aattggtatg ccattaaaaa tactggttgg tattgcatta tttatcatat caataccttt
    13921 aattgtcaat caaataagtc atttattaag tcaaatacca aagatgtatg agggaacttt
    13981 tgcactagct cctatgtttt ttatgggttc aacagataaa acagaagaag ctactccaaa
    14041 gaaaaaggga gaacaaagaa aaaaaggtaa tatagcaaag agtaaagaat tacccattgc
    14101 aatgactcta cttgcattca ctttattagt acctacatta ttttcatatg tagtgaatac
    14161 acttaaatct tctcttaatt attttttgag tctagatttt tatatgaaca taaattattc
    14221 aagtatagag aaattagtta tagcaggatt aatggatttt tttaagatat ttttaccaat
    14281 agctattcca ttcttagttt taggtatagt cgcaaaccta tttcaagtag gaatactgtt
    14341 tactggtgaa actttaaaac ccaatttatc aaaattaaat ccaattagtg gattcaaaaa
    14401 catgttttct atgagaagtt tgtcaacctt aataaaagat acagcgatta tatctatttt
    14461 ggcttacata ggatatacat tcttccaaga taattattta gatatattaa agttaggtaa
    14521 catatacctg ccaactttaa tgtacacagt taaagattta gtatattcaa tattaagtaa
    14581 gatttgtgta gctatgatag ttatagcagt tgcagattat gtttatcaaa gatacagtca
    14641 taaaaagcaa cttagaatga caaaacaaga agttaaagat gaatataaaa actcagaagg
    14701 agacccagaa ataaaagcta agataaaaca aaaacaaaga caaatttcgt ctcaaagaac
    14761 catgcaagca gtaccaagtg ctactgtaat agtaaccaat cccacccact tatctatagc
    14821 agtgagatat gaaaaaggga aagaccaagc tccagtagtg gtggcaaaag gtgcagatta
    14881 tttagctttt aaaataaggg aaattgcaaa gggaaatgac atacctataa tagaaaataa
    14941 acctattgct aggttgttat ataagcaagt agaaatagac caagaaattc cagaggacat
    15001 gtatcaagct tttgcagaga tattagtagc agtatataaa atcaaaaata gatacaaagt
    15061 ccccaaaagg taggataagt aatgaataag tttaatttaa aggaaaatat ggacgtaata
    15121 gtggcatttg gtgttgtagg gattgttctt atgatgatag tgccgttacc tacatttata
    15181 ttagatattt tactggcaat taacataagt ttatctgtgc taattttact aatgacacta
    15241 tttacaacaa atgtacttga tttatctata tttccaacag tgttacttgt gacaacacta
    15301 tttagattgg gattaaatct ttcatcaact agattgattt taacacaggg atatgcaggt
    15361 gaagttatag aggcatttgg aagttttgtt gtaggtggaa attacgtagt aggaataatt
    15421 atattcttaa ttatattaat tgttcaattt gttgttataa ccaatggttc tggaagagta
    15481 tctgaagtaa cagctagatt tacgttagat gccatgccag gtaagcagat gagtatagat
    15541 gcagatttaa atgcaggagt tatagatgac aaaactgcta gaaaaagaag aaaagagctt
    15601 cagcaagaag cagattttta tggctctatg gatggagcaa ataagtttgt aaagggagat
    15661 gccatagcag gtcttatagt aacagcaata aacataatag gtggaattgt aataggagct
    15721 gtaatgctga atatgactct tatgggtgca gcacagacat atgtaagact tactataggg
    15781 gatggtcttg taagccagat acctgctcta ttaatatcta catcagcagg tataatcgta
    15841 acaagagctt ctactgatga aaactttggt ggattagtag gtaaacagtt aacagcaata
    15901 ccaaaagcag taatgatgac tggctgtgtt ctaatcgtac ttggtatgat gcctggatta
    15961 cctaaaccag catttttttc actaggtata ggtgctatag cagcaggtta ctttttagga
    16021 agagaaaaga aagatacttt agaagacttg gagtttgaaa aacctgatat ggtaaatcca
    16081 gataaaaatg aagtagaaga tgtaacaaac ttaataaatg tagaatcaat agaagtagaa
    16141 attggatatg gtttaatatc tttagctgat gaatcagcag gaggagattt acttcagaga
    16201 atagcatcaa taagaagaca atgtgcaata gacatgggta tagtcgtaca acctataaga
    16261 ataagagata atttacagtt aaatccaaac caatacaata taaaaataaa agggaatgta
    16321 gttggtgggt atgagataat gcctaatatg gttttatgta tggatcctat gaatcaagga
    16381 tttagtatat ttggaataaa gacaattgag cctacttttg gaatagaggc actttggata
    16441 gaaagtgaca aggtagaaga tgcagagcta aagggttata cagttgtaga ttcatccaca
    16501 atacttataa ctcacttact agatataata aaatcaaatg cacacgaatt actgggaaga
    16561 caagaagtta agactattat agatgcagca agagaaaatt atagtgctgt agtagatgaa
    16621 ttgataccag atttaatgac acttggtgaa attcaaaaag tacttaaaaa tctgttaaga
    16681 gaaaaagtaa atataaagga tagagttaca atacttgaga cacttgcaga ccagtctaga
    16741 aatacaaaag atatagagct tcttactgaa tatgtaagaa tagctttggc tagaagtatt
    16801 tgtaataatt tagttgatga agataaggca attgtagtgg ctacattgtc tccagaaact
    16861 gaagaattgg tatctaataa tttacaaagg tctgtaaatg gaacttatcc agcagttgac
    16921 ccagaaaata caaataaaat atttgagagt atacaagaag taatgaacaa tgtatatttc
    16981 aatcataata taccagtaat agtagtatca ccaaagataa gagctccatt tagaaaatta
    17041 gttgaaattg tatatcctaa tttgactgta ttatcattaa atgaaatacc taacgatatt
    17101 aaaatcaaag cagaaggtgt ggtgagcata tatgatgacg aaaaagtata cagctaacac
    17161 aattcaagat gctatgaatc tagccaaaat ggaattgggt gacaatatta cattaataga
    17221 taaaaaagag gtaagaaaat ctggaataag aggcattttt tcaaagaaag atattgaatt
    17281 gactattgga tgggaaaaga aagataattt agaacaaaaa gatttaaaac gagagataga
    17341 acaactaaag tcaattataa ataatatggg gtttgataat aaaaatgata atgatataga
    17401 taaaatatgc aagaatttac taaacttaga tttaaatcaa gaaattgtag aatttataaa
    17461 agctgattta caagaaatga agttcaatgg aattgataca agtaaaaatt tagtagaaag
    17521 tctaaagaaa aagattaaaa tagaaaatca agcaataaat ggtaaaattg ctttagttgg
    17581 accaccagga gtaggtaaaa cgactacaat agcaaagttg gctgccaaat tagtttttga
    17641 ggaaaataaa aaagtaggag taataactat agatacatac aggattggtg ctgtagaaca
    17701 gttaaagata tatactgata taatgaatat tccttttaaa ggggtaataa gccctgatga
    17761 gatggaattg gcacttgatg agatgaaaga ttgtgatgtt gtattaatag atacaacagg
    17821 tagaggctat aaaaactcta tgcaaatatt agaaatcaaa aatcttatag ataaagctga
    17881 aactgataat atacatttag ttttgaactg tacaacaaga gaaagtgata caaaagcaat
    17941 tatagattca tatagaaatg tgaattttaa aagcttaata attacaaagt tagatgaaac
    18001 aataacatat ggctcaatat ttaacataat gaattatgca caaaaaccaa tatcatatat
    18061 tacaacagga caaaatgttc ctgatgatat tataaaacct aatgaagata agattataag
    18121 attactatta ggagttgaaa gtatatgata gaccaagctc aaattttgag aaaaatggca
    18181 atggaaaaaa gaggttctga tgagattata gtagagaatg agaatatgcc taagataatt
    18241 acaatagctt ctggtaaagg cggcgttgga aagagtaatc tcgctacaaa tttgtctata
    18301 tgcttaacca aacttgataa aaaagttctt atattagatg cagatatagg aatgtctaat
    18361 atagatataa taatgggggt aaatgtaaaa gggactataa ttgatgtaat taatggcgaa
    18421 aaaaatatag aagatataat ttcacagact aagtatggtg taaatataat atctggaggt
    18481 tctgccctta atcatataga agactttaca gaagctcaaa gaaataagtt tatacatagc
    18541 atagaacaga ttcacaatgt ggattttata ataattgata caggagctgg tatgagcaaa
    18601 agcctattgt catttatata ctgtagtact gaatttttct taataacaac tccagaaccg
    18661 acatcattga ctgacgcata tagtttatta aaagctataa gtaattttgg cataaagaaa
    18721 agtgcaaata taataatcaa tagggtaatt gatttagaag aagcgaaatc tacatacaaa
    18781 agaataaaaa tggttgtgga caaatttcta aaccttaatc tggaattgta tggatatata
    18841 atagatgaca aaaaagtagg tgtatgtgta aaaaaacaga taccattcat tttagaatat
    18901 cctacaagta ttgcatcaaa atgtattata gctatagcaa agagaatggt aagtcagtca
    18961 agagaagata ttgttgataa taaaagtttt ctaagaaaga tgtttagtat atttaaaagc
    19021 taaggaggcg tagttaatga atagagaaga attaataaaa gagaatatgc ctcttgtaaa
    19081 gagtatagca agaaaatttt ttataccagg gaaaggtttt gaatatcaag acttagtaaa
    19141 tagtggagtc attggattga ttgatgcaat aaataaattt gatactgaaa aaggagcaaa
    19201 gttttctagt tactcttata taaagataaa atctgctata ttagatgaaa ttagaaatca
    19261 aagtccaatt tctaaacata atctaactaa agtaaataag tataatagag tggtagaaaa
    19321 gttgcaatct aaattattaa gagagccaac ttcatatgaa attgcaaaag aattaaaggt
    19381 ttcagaaaaa gaacttcatg atatcgaaag taatatagat atgttaaata ttgtatcatt
    19441 aaactatgta atctttgaag atacaaatga aactgtacaa gatgtaataa gtgatagaga
    19501 agaggaagct ccagaaaata taatagaaga agaagaaaag ttagagattc tatccaaagc
    19561 aattagtaat ttaaatgaaa gagaaaaatt aatactttca ctgtactatt atgaagattt
    19621 aaatctaaag gagataggga aagtgctagg tgtttcagaa tcaagagttt cacaactcca
    19681 cagaaaatca ataagaaact taagaaacaa gataaaagaa cttaaatatt ctatatagat
    19741 ggtgattgat aagatgatac aaattatatt aatagtagtg tctattttaa taatagtaac
    19801 tatagttata aatttacaga aggaatcaaa aaataaagat aacagatact ttgataaaat
    19861 atttgagcaa gaatatgaag caaatgatag attaataatt gcagaaaaaa gaagattctt
    19921 tttagacaaa gtacttgcag aaaaaagaat taatagtcat ataaaagttt atgaaaatta
    19981 taacctaata aaggctaatg aagaaagaaa ggaaaacata tttatagaat caaaaaagga
    20041 agtaagacaa gaaaatgagt tatctcttaa agtgaaacat cttataagtt caggttatga
    20101 tgatgtggaa atatgtaaga tgctagatat tggaaagggg gaactgttat taataaggag
    20161 ttgttacaaa atataaggat attattatgt gataaaaaag tattcttagg aataggtatt
    20221 ggtatattaa tatcaacttt atgtataatg ctttttagta ataatgatat gagtaaggca
    20281 gagatagaaa aaaaggccag agcgttaggc atgaaatata caagtgagat gaaagtttta
    20341 gaatagatga tactgtttag acatttacaa aaaaggaggt cataatgtga taagaggatt
    20401 atatacagca gtttctgcta tgataactaa tcaacaaaag caaaatgttg ttgtcaataa
    20461 cctagctaat atggatacaa atggatataa aagtaaacaa cttttgacta aaagttttga
    20521 tgaattaaag ttggagggtt acaataacta cgcaaatgga actaaacaaa agcagattat
    20581 aggcgatata agtccaggta tttctatgga tgaaactatc acaaatttta atcaaggacc
    20641 tataaaaact actgataata aaatggatgt agctattcaa ggtaagggat tttttctagt
    20701 tagtgatgca gctggaaatc aattttacac tagaaatggg aacttcagaa cagacaatca
    20761 aggtgacctt ataacaagtg aagggtatta tgtacttggg acgaatactg ctactggtgc
    20821 tgtaggtcat ataaatgtag gaaatcaaaa atttgaagtg gcaaaagata atacaatatc
    20881 actagacgga aatgctatgt ataaatttaa tattgtagat tttaataact acaataactt
    20941 aaaaaaagaa ggagataact tatattctgg aggaggagct atgaaagtaa ataatgcact
    21001 tacaatacaa agtgcgatag agtcctctaa tgtagatatg gtatctgaaa ttaataatat
    21061 gatgacaata tcaagagaat atgaggcaaa tcaaaagata attcaggcta tggattctaa
    21121 attagcaaaa atagcaagtg agattggttc agtaagatag gtaataggag gtgaattttt
    21181 atgtacacaa tgttttatac tagtaaaaca gctttatcag caaatcaaag taaactagat
    21241 ataatatcta acaatatagc aaattctgat tcaacaggat ataaaaaaat aaatatggaa
    21301 tattctgatt tagtggatga agtactaaat agaccatcat accctacaaa tggtaaagat
    21361 atatcgactg gaacaggagc taaggcaaca gctcccatga gaaattatga acaaggtgca
    21421 ttggctccta cagatagtaa gagtaatttg gcgatagatg gagaaggatt ctttagagtg
    21481 ataagaaaag atggtacata tgcatataca agaaatggtg gatttaatgt agatgcttta
    21541 ggtaagattg tagatgataa tggaaatata ttagatgtcc aatttgatgc aggatataac
    21601 tataataata caaatataga tagtacaaac ctgacagttt caagaaatgg agagctattc
    21661 gtagataata aaaaaatagg aaaaattaac ttatatcaac ctataggaac tcaaaatttt
    21721 atatctgaag gagatagttt atttgtcgca actgatgcag cacagattaa acaagttgag
    21781 aaagtcgata tagttcaagg ctatagagag aggtcaaatg tttctttaca agaagaattt
    21841 gttgacttaa tagccactca aagagcattt caaatgaact caaagggaat aaagacagca
    21901 gatgaaatgt ggcaaatagc gaataattta agagctaaat aattatgtaa ggagaggaat
    21961 attcatgaat gatatgaaag agtacgattt tggcaaacct cagagttatt caagagaaaa
    22021 tttaacttgt ttaaattttt tattagctga attttgtaaa aaatataaaa actatataat
    22081 ttatgaactt aagtgtaatt ctagtatggt tgttgatagt atagaccaag taaattatca
    22141 gactttttta gatagagtaa cttcttcatg cgtagtagta caaaatgcaa tggaacctgt
    22201 tatgaaaaac ttttctttta gaatagatag aagtgtagct gatatgtgga ttgatataac
    22261 ttctggagga actggagttg taaaggataa tgatagagag cttacagaac ttgataaaaa
    22321 agtgctttta caccttatgg atgatttagt aagaaatatg cacttatttg agggttttga
    22381 aaatatacaa gtattggata cacatacaca tattaatttg ccacaattat gttctccaac
    22441 tgcaccagta tgcgttgtag atttaaaagt gctaaatgat aatgagtgta ttggaagtgt
    22501 ttctctttgt tttccataca atggtttaga ggctctgtta gaaagtatat ctgttataga
    22561 attttttgat aatgatgtag gagatgattc agaggaattt acccaaaaaa tctataatag
    22621 tgtatctaat atagaattat cagttattgc agagatagga aaagtatcaa ttaatgttga
    22681 ggatttactt aaactagaag ttggagatgt aatagtaaca aataaaaaaa taaatgatta
    22741 tatagatata tttgtagaaa attcaaaatc atacacagca acaccaggat ttatatcaag
    22801 taaaaaaggt gtaaaaataa atgatgctgt tggaaaagag gtgtagttat ggacaatagt
    22861 aatcttattt cagatgaaga agcaaagatg ttattaaatg ataaagttga aagtgaagtt
    22921 gatggagatg atgagttagc cttagatgtc tctgaagtta atcgaaataa tatacaaagg
    22981 atacttgacc ttaagttaga attatctgta gttataggaa gaactaagat gactctaaga
    23041 gatattttaa atttacataa aggttctctt attgaactgg atactttagc agaccaagat
    23101 gtggaaatac taatagataa taaggttttg gcttatggaa aagtagtggt ggtagattta
    23161 aattttggtg taaagataac aagtatagta agtgaagaag acatggtaaa atctcttgtt
    23221 tagagataac atcaattaat acttaaggca tgaggtggta cttattttaa acttaaatga
    23281 tataaaaaaa gatatactaa aactcaataa aaaagactca tgttctctat ataatgaatc
    23341 tattaataat ataaataata attgtatatc aactggatta aaatttttaa aacaagcaag
    23401 agatttaaac ccagatgatg ttgatatatt aaatctcaca ggtcttgtaa acttactaaa
    23461 atgtaatttt gatgatgctg tagaaagttt ctataagagt tcactttgtg aaaaaactgc
    23521 tctgtgtgaa aagtatataa acatgcttac atctgaggag tttttaattt tctttgaaaa
    23581 gtacaatcaa cttataaggt taataaataa aggaatgtat aaagaagcta tacaagggtt
    23641 taaactcata actgaccaat actgtgattt aatagaacct tatgagttgc ttgccttact
    23701 gtatgacaag gaagaagaat atataaaatt tgatgaatgt ttagaagtat tgaagattat
    23761 tgataaggaa aattatttat taaatcaaga aaataacaca ttagatatgt ataatagtgc
    23821 taatatacaa actaaaagta atataaaagt agatgattat aagaatgagt tcttacataa
    23881 aagtagtagt aaccataaaa ttaatgatta taatgatgta actaaagaaa agagttcaag
    23941 caaaaaccag aaaagttcag attctaataa gaaaaaaagt attaaaaaaa ataagtacct
    24001 atatatagcc ataatattag gaatattatt agtaggacaa agtatatata attcttataa
    24061 gatgaataat ttaactgata aaatatctaa aaataatgaa actaaaaatg aagagtcaat
    24121 agattctaac ttgactaaga aagaaaataa tatagaagaa aatgaagata tgagtaaggc
    24181 ttctaatata aataagagta ataaaaacat taataaagag gatgatgtaa aatctaaaaa
    24241 aaatactgta gaaaatgaag atgataaaga ttctgactta tttacagagg atgaattgat
    24301 gtctaaggcg aatactttaa aatcagaaaa gaaaactaaa tcttctatac aactatataa
    24361 gaaagttgca gatataggtg aaaataaagg gaatacatct gaagcaactt atcaagttgc
    24421 tattctcagt gaaaagttaa aagactataa aactgctgaa gaatattata aaatgtatgt
    24481 agaaaattac tcagaaaaag atgcttattt tgatgaatct tattataatt taggtatgat
    24541 gtactataac aatggtgatt taaaaaattc taaattgaca ttaaagaagt tggttaataa
    24601 ggtaccaaat agtatgtata ataattcaaa agtaaaagaa attttaaaa
//
LOCUS       DAMJVK010000054        24536 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.055, whole genome shotgun sequence.
ACCESSION   DAMJVK010000054 DAMJVK010000000
VERSION     DAMJVK010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24536
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.055"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(115..951)
                     /gene="nadC"
                     /locus_tag="PC414_002281"
     CDS             complement(115..951)
                     /gene="nadC"
                     /locus_tag="PC414_002281"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HDF2464674.1"
                     /translation="MNYLIIDRMIQDALIEDVPSEDITTNSIVDENSKSTVDLICKQD
                     GIIAGLGVFKRVFEILGDVDVKLYKNDGDRVKNREKIAVLTGSTRNLLVGERVALNYL
                     QRMSGIATITSRYVEKLEGTNTKLLDSRKTIPNLRILDKYSVKVGGGCNHRFNLSDGI
                     LLKDNHIGAAGSVKKAVELARKNTSFVRKIEVEVETLDMVKEAIEANADIIMLDNMSL
                     EMAKQAVDIINGRAIVEFSGNVNLDTIEDIGKIGVDVVSVGALTHSVKALDISMKNLR
                     NI"
     gene            complement(932..2233)
                     /locus_tag="PC414_002282"
     CDS             complement(932..2233)
                     /locus_tag="PC414_002282"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HDF2464675.1"
                     /translation="MNLEQDVLIVGSGVSGLYCALNLDKSLNVLVVSKSTIENNNTYL
                     AQGGISTARNIDDIESFVEDTMKAGQYKNRVEAVQVLADESIENVGQIVEYGMPLDKE
                     NGEIDYTREGAHSVNRIVHSKDNTGEVVFKTLLKEAKTRENITLIEDAYLLDILKDGN
                     KCIGARIFKSKKEIHVFSKIVVLATGGIGGLFKNSTNQRHLTGDGIAIALRNNIKIEN
                     LEYIQIHPTAFYEENNEGRRMLISESLRGEGAKLLNKNKERFVDELLPRDVVSKAIFE
                     QMEKDKLPYVYLDATHLDSEYLINRFSFIYNECLARGTDITKECIKVSPAQHYFMGGI
                     HVDLDSKTSMENLYAVGEISCTGVHGANRLASNSLLEGLVFSKRAAKNINSVIDNVKV
                     KFIDVPDMDIDIEQVKKENKILVIKEIERTSEDFGDELFDY"
     gene            complement(2246..3160)
                     /gene="nadA"
                     /locus_tag="PC414_002283"
     CDS             complement(2246..3160)
                     /gene="nadA"
                     /locus_tag="PC414_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HDF2464676.1"
                     /translation="MDKDLTYQIKELKKEKNAIILAHFYQPPEIQELADAVGDSYYLS
                     EIARDCKEEVVVFCGVRFMGESAKILSPEKTVLMPVSNAGCAMADMVDEEGVIKLKQQ
                     YPNALVVCYINSTAKVKAHCDVSVTSSSAIKILENIDNKEIIFLPDKNLGGYIAEQFP
                     DKNFIFWDGYCKYHNNIRAEEIIELKDKYKNAEVLVHPECKKEIRDLGDYVGSTSGII
                     KYATNSKNKDFIIATEEGILHELKKNNPNKNFYIPGGKILCTDMKKTTLENLYSTLKN
                     MENEVIVEDEIMEKALNSLLNMHKLAEG"
     gene            3578..5035
                     /locus_tag="PC414_002284"
     CDS             3578..5035
                     /locus_tag="PC414_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HDF2464677.1"
                     /translation="MTTFLIGLAILLIGGALYGAYCEKVFGPDDRKTPALAQSDGVDY
                     VPMKKWKNSLIELLNIAGTGPILGPIQGILFGPIAFILIPIGCVFGGALHDYMSGMIS
                     IREKGAQMPSLISRFLGNKVFQVYNIFLCLLMLLVGAVFIYTPGDLVVTQILNMKSTI
                     NNPVVWIVYGLIFLYYLCATLFPIDKIIGKVYPIFGAILLLSAAGVGIGIFTQGYDLA
                     NLSLANWKGIHPDGIPLIPTFFVTVACGIVSGFHSTQATLIARSVSNEKEGKTTFYNM
                     MILEGLIAMIWAAAAMGIYNKGIPKELVGSPDVIGLVARDLLGSIGGIIAIIGVIVLP
                     ITSGDTALRSLRLMLADYFHYDQKEKKHRVILSICIFIPVIAILIFAKLSASGFNILW
                     RYFSWSNQTIAIFAFAMITVYLIIKEKNYIISLIPGMFYSFVIFSYIFNAQIGFNLNI
                     NISYVLAAIFTVLYAILTVRSGRKLKSKADTKLAD"
     gene            5258..5686
                     /locus_tag="PC414_002285"
     CDS             5258..5686
                     /locus_tag="PC414_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464678.1"
                     /translation="MNNKLHELEKNLPETSCSFCTHLSFKGPSLDYRYDIRCVISDTK
                     PDFRGCCEYFEPEYTELNTGDLDNLYINFLETCLKVNYNDYINSMYWKLFKERTLLEN
                     NFKCSICGSTENVDVYHVNKNLGRETSKDVVVKCDKCNCK"
     gene            5746..6054
                     /locus_tag="PC414_002286"
     CDS             5746..6054
                     /locus_tag="PC414_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HDF2464679.1"
                     /translation="MKNNNLNCAATNCAYNTSGYCYAGSIKVDGMQATTTENTYCASF
                     EDKYTSGITSRSNETNQVDTDNIHCEAVKCKYNKNELCKAEKVHINERNASCETFEMK
                     "
     gene            complement(6201..7223)
                     /locus_tag="PC414_002287"
     CDS             complement(6201..7223)
                     /locus_tag="PC414_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDF2464680.1"
                     /translation="MEDNKGLARDSSKYKIVSISVVVIILITFWYMLNMVLLTFIMTF
                     IFYNLLVATRKRIKKFSSLNIPDSLIIIVLYALFAILLVLISYAVVPIIIVQLTELSR
                     VFSDFDVNQFAQSLGPKLYPIVSKLDFNKYISQAGLLIASTATKVGSFGVNILLAFLL
                     SLLLLLEKNEIKNFGDKLSDSKISFIYNSLVFFGKSFVKNFGEVMKVQVMIAFINSVV
                     SMIFLGFMGFPQIWALGFMIFVLGLIPVAGVIVSLIPLTVIAFNTGGITKVFGVLLMI
                     CIVHAVETYILNPKLMSNRTKLPVCFVFIILLVGEHYLGVWGLLIGVPIFMFLMDILG
                     VKFTSR"
     gene            complement(7398..7904)
                     /locus_tag="PC414_002288"
     CDS             complement(7398..7904)
                     /locus_tag="PC414_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HDF2464681.1"
                     /translation="MELWDLYNADGIKTGNVIKRGNSIEEGYYHLAVEVWILNSNSQI
                     LIQKRSKSKKTLPNMWGMTTGCIVSGEDSLEGAIREAKEEIGIDITKDEMKVFRSMIH
                     EDTLWDVYLVKKEYDISKAILQEEEVSDIKWVSTDEIRQLLKEGLFFEYPEIYELLYE
                     IDNNKLNI"
     gene            complement(7964..8665)
                     /locus_tag="PC414_002289"
     CDS             complement(7964..8665)
                     /locus_tag="PC414_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464682.1"
                     /translation="MGKISLKSMIRLFSGFFIFAISSVLMINAHVGLMPWDVLHQGLS
                     IKLGITIGQASIMVGVVIVILDAVFGENIGWGTLLNMTFIGIFIDLVIFSGVIPHASN
                     TYIGVFMVVIGIILAAIASFLYLGVCLGSGPRDGLMIALQKKTNKSVRLVRTILEILA
                     LVVGWLLGGSVGIGTLVSALGLGYVLQIVFRIFKFDTKLLKHRFIIDDIREWKEKKSN
                     EHKCKSSIVIKNEQN"
     gene            complement(8883..9953)
                     /gene="buk"
                     /locus_tag="PC414_002290"
     CDS             complement(8883..9953)
                     /gene="buk"
                     /locus_tag="PC414_002290"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HDF2464683.1"
                     /translation="MTYRILAINPGSTSTKIAVYDGEEQILVKTIDHPAEEIAKYNTI
                     QDQFEMRKEAVLNILKENSIDLKSLSAIVGRGGVLPPVKSGAYLVNEEMIDVLRHRPV
                     LEHASNLGAVVAHAISEPLGINSYIYDSVAVDELIDVARISGLCGMDRSSAGHALNTR
                     AMALKYAKDKGKDYKSLNLIVAHIGGGVSIYLHEKGRMVDMLSDDEGPFSPERSGRVP
                     ATKLVAACYSGQYSEREMTKKIRGKGGIVSYLNTVDAREVEKMIAEGNEEAKIIYEAM
                     AYQLAKGIGELATVVDGKVDAIIITGGIAYSEMFTSMVKKKVEFIAPVEIMAGENELE
                     SLAFGTLRVLNGEEEARIYSEN"
     gene            complement(10006..10581)
                     /locus_tag="PC414_002291"
     CDS             complement(10006..10581)
                     /locus_tag="PC414_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HDF2464684.1"
                     /translation="MTKSILLVGVGGQGTILASKLLTMGLMEAGYDVKMSEIHGMSQR
                     GGSVSSQVRYGDCVHSPVIEIGGADILVSFEKMEALRWFNYLKPEGKALVNNHRIDSM
                     TVLIGGAEYQESDIDAELSRLNAKVINAADKAEELGNSKIMNVILLGCLVKSMQLESI
                     DWEKIISENVKPKFVELNIKAFHEGMEMVSK"
     gene            complement(10583..12370)
                     /gene="iorA"
                     /locus_tag="PC414_002292"
     CDS             complement(10583..12370)
                     /gene="iorA"
                     /locus_tag="PC414_002292"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HDF2464685.1"
                     /translation="MKQLMTGNEAIARGAYEAGVKYASAYPGTPSTEILENIATYKDA
                     IVAEWAPNEKVALEAAIGGSIAGARTMASMKHVGLNVAADPIFTYAYTGVNGGMVLIT
                     ADEPGMHSSQNEQDNRMYAKFAKIPLFEPSTSQEAKDMIKEAFEVSEKYDTPVLYRVT
                     TRLCHSKGLVECYDREEVEIKEYVKNAKKMVTVPANAQIRRGVVEERMEILKKFSNET
                     DLNYYEINDTKIGVIASGMCCNFAKEVFGKNASYMKLGFTNPLPYEKIKEFAEKVDKI
                     YVIEENDPFIEEQIKAYGIDCIGKDVIPPYGEMTPDVLRKAIFGKTNDTIEYKSELVT
                     PRPPSFCAGCPHRGFFYELGKRKNLIVGGDIGCYTLGFAPPYNGIDYVVCMGSAFGTA
                     HGAQKVLNMKDDNEKRLVGVLGDSTFFHTGINGLLDVVYNRGNSISVILDNRITGMTG
                     HQENPGSGYTLQGAKTKEVDIEGLVKACGIEHVRVINPNNLKEVNEALDWALAIEDEP
                     SVIITRWPCVLKKFSKEDIEEFNNPFKTKCKVDHDKCIGCKLCLKTGCPALSFDKENK
                     LSNIDRNQCVGCGVCAQVCPKQAIVKEEK"
     gene            complement(12431..13690)
                     /locus_tag="PC414_002293"
     CDS             complement(12431..13690)
                     /locus_tag="PC414_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF2464686.1"
                     /translation="MSQSMVGKHAMWPKENDVIFSISGRAQAAEKAFGMDNVINATIG
                     ALMDDSGKLITMKTVYEEYKALDNCEIGAYAALEGQPDYLEAVKKVFFRDYLPEGHIR
                     VLASPGGSGAIKLAVWNYTNEGDEVLTSDWFWSPYVSIAEEANRKVVNYQLFDENRRF
                     NFESFKEKFVNIAEKQGRVFTIINTPAHNPTGYSVADDEWDKILDLSKEVAKDKDKKI
                     IFFVDSAYIDFAGDDDVCRKFFKKFSNLPENVLVLVGFSMSKGFTAYGMRMGAIICIS
                     SSEDVAEEFHYSCVHSCRANWSNCNRSAMAVLSNIVNDPKKFKEYEDEKEIYKNMLTR
                     RADVFVKEAERVGLEILPYIAGFFVSIPCDNPKEVCEELTKHNLFAVPLKMGLRFAVC
                     AVSEDKCKKAPSIIKEALESLEVKINN"
     gene            complement(13952..15952)
                     /locus_tag="PC414_002294"
     CDS             complement(13952..15952)
                     /locus_tag="PC414_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2464687.1"
                     /translation="MILLRKKIGIIASDIELKERIEELYREDVENGTIIIDILNLDLM
                     ENQGRILVEKGAQAIIGRGGGYSLVIDTVNVPVIPMNMKSTDLLRAIEIAKKYSKKVV
                     LILGDNEVSFDYVGWRNVISTEITEEWFESKYEIRSKVVKYIDQKDEVVIVGGGLACS
                     FARQYGIDSVFATASDESIREAVEYCKKLLDTLGEEKFNNEVLRNILDGIKDGVIAID
                     SNGSIILYNESAKNMLKVERKCALNKYILDVFPKMEWMLDCLHEKEDVEDRKIRNINN
                     LIVNTRTTLIKVDNSTYGVLGIIQDITKLQNLERKIRFDLNQKGLYARYTFDDFLFKD
                     KLTKEFIEEAKKIGKSDYTTLLYGESGSGKEIIAHSIHNISKRKDRPFVAINCATIAE
                     NLLESELFGYEEGAFTGARKGGKRGLFELAHGGTLFLDEINSLSFNIQTKLLRVIEER
                     QIMRIGSDYIIPLDIRIIAATNESLTEKIVMGTFRADLFYRLSSLEINIPPLRDRRED
                     IIPLFNNFVNEVLKDDGLNGINSIDENFVLTKDEMDKLYNYSWPGNVRELKTIAQKYV
                     VTGKIKLRQDRDFKTKKLLPNSEVDKFNSETTASVEVQDESINISKINDGKISIDIKE
                     VNKYVEEKIISMLFAQGLSKNEVAQVLGISRTSLWKKYNKNI"
     gene            complement(15960..17847)
                     /locus_tag="PC414_002295"
                     /pseudo
     CDS             complement(15960..17847)
                     /locus_tag="PC414_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897561.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
     gene            complement(17874..19715)
                     /locus_tag="PC414_002296"
     CDS             complement(17874..19715)
                     /locus_tag="PC414_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2464688.1"
                     /translation="MEEIDDTILDSMFKLTLNERLLTNNDFIENKIDVVKNLIDCYKT
                     EQNSLMLLKSNFLLALLYGIQGFSEKMKEYILISCKYIDKCLPKDYKFLARFYSQIAI
                     LSLKNEDVNKANLYMDKFNLICDENNFLIEEIIFKSQLIYIKASKCIESSVIIKEIET
                     LYIKIKEINDFNCKKIYFFIIGKIYFLFLDDALIAKVNFLKARKYADLLKDMQVSSLC
                     NIKIGECECSFENYECSMEYFNEVISNKKYRNVNIIQKYRASNNITKILIKTRNYPVA
                     INNLAKSEVYLEKIKNHSLKEVEKFDLFISLAMYYAKSNEKSFEQSICYLDRAKNILN
                     MIKNIEEYIIYDLEIIYHQAKIYYIFGYYDNALNTNKKLLEKSKNAKNYNYVKLAYKS
                     IYLCFEKIQNYEMSMKYFKMYYELKMMYIKARNRNYIDSLNYRHEYIEKEINNMRKVK
                     LDLDKKKYVDHLTSAYNRTFLNDFLEKQEVSEYCTAFMIDVDYFKEYNDTYGHYNGDI
                     ALKNITIIIKKYLKKDMLMIRYGGEEFLILSVCKDYKKSKIFGRKLCRIVKKFLNNNL
                     TVSIGIDTCKNSSIDIKEIIENADKALYKAKESGRNRCLHYHDFKNF"
     gene            complement(19846..20265)
                     /locus_tag="PC414_002297"
     CDS             complement(19846..20265)
                     /locus_tag="PC414_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3785 domain-containing protein"
                     /protein_id="HDF2464689.1"
                     /translation="MSLYKFIYDDKEYLLKEENCSALINDEENPVQGVSISKIIDILN
                     EAEEVDFDVEYYQEACPLCLEGVKEKKKFFPFLEYHFYIFSKDGEYVISNISVDYKGL
                     SFNKLSRANKVDNSYIVSVIICENCQDYIVQIENCIV"
     gene            20578..20760
                     /locus_tag="PC414_002298"
     CDS             20578..20760
                     /locus_tag="PC414_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464690.1"
                     /translation="MYKDKSDECIHLMTAYIDSISGYYSFIDTQLEDFMMKYGENIVD
                     SNLHSIMMLLCKWGLS"
     gene            21089..22111
                     /locus_tag="PC414_002299"
     CDS             21089..22111
                     /locus_tag="PC414_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDF2464691.1"
                     /translation="MDYNDLLKSARENFNGTCKVCKICNGLACAGDVPGMGGKGSGSS
                     FIENRKSLEKIKINMRVIHNVSKPDTSIELFGRKMSSPIFAAPVSGTILNMGGKVSEK
                     EYIEPVVRGCSNSGIYAMVGDTNVDTFLLDNLDVLKDNRGNGIVFIKPWNNSKIIDKI
                     RLSEEAGAFAVGVDLDACGLINNQFQENPFSPKTIDEIRELVESTRLPFIIKGIMTVD
                     DALMAVESGASAIIVSNHGGRVLDYTPGTCEVLPDIAKSVKGKITILVDGGVRTGVDV
                     VKMLGLGADAVLMGRPFVTASFGGGLDGVEFFIEKVRNELCETMILTGCQNVKDIDGR
                     VIWNLL"
     gene            complement(22204..22806)
                     /locus_tag="PC414_002300"
     CDS             complement(22204..22806)
                     /locus_tag="PC414_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HDF2464692.1"
                     /translation="MLTKLRKIVAIMCCLLLTFTSVAFADTSKEKGGSKETVPEKNLD
                     SFTLSGYIYTYENAPQEIRDEHKQNCEEINIDPKPDDEIFVPESANLMNEDNSKGVYA
                     SYTVSYNIGAKTITIMSNEYLTISTTKVIRKGNSGKEVKAAQIMLTLLGYNVGIDSSF
                     GSKTYNAVVSFQKKYGLSADGIIGPATWDKLGRLTDPTLS"
     gene            complement(22823..23971)
                     /locus_tag="PC414_002301"
     CDS             complement(22823..23971)
                     /locus_tag="PC414_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease"
                     /protein_id="HDF2464693.1"
                     /translation="MNNYMLYVFENLIKTTIVCSLGICLLLFLKRYLFKKFSKRFNYY
                     IWLIIVFRMLLFLFNYTIVYEVKEPKENALGNNITQIDISTDNNLMLYVAYLWLFVTI
                     VIAVYTFIKYTRFKNLVVDVSYDIEDNDINCIYKNLLKELNIKKKIELRGSDELISPA
                     GMGLFKSYIFLPDYPYSKDELTWILKHELMHFKNKDILIKFLVLSVRIIYWFNPLVYV
                     MSNKVNLDCELCCDESVLVECSLKDKKEYALALIKSIKFSKNYNSKILTTEFDKTNLE
                     KRLESIVKKKGKSGILIAVLVFMTSITSFVEVDAQVRIDEGNNGMNNNINEFLLNKTT
                     DKIINIYYKDAPNEIRKFYEDKCELEGKIPRDYDIIEMDMKDYNQLIK"
     gene            complement(23964..24341)
                     /locus_tag="PC414_002302"
     CDS             complement(23964..24341)
                     /locus_tag="PC414_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2464694.1"
                     /translation="MMIRKIPQAELKVMKFIWKVDVTVTSKDVIEAMEQKYGWKQTTT
                     LTLLSRLVKRGFLDAQKIDRYTHYTIRVKHKDYLSFETKDFLSNIHDDSLRSLISALH
                     DDENIGKDKLDFLESYFDNLKDE"
BASE COUNT         8048 a         4184 c         2606 g         9698 t
ORIGIN      
        1 attgattgat ttttttctat ttttattaat tttaatgaat aaaaaataga ctccttaaat
       61 ttataaaaca aatttaagga gtctatttta attaagttta tttcaatata aaatttatat
      121 atttctcaaa ttcttcatac ttatatcgag agctttaaca gaatgtgtaa gcgctcctac
      181 agaaacaaca tccacaccaa tttttcctat atcttctata gtatctaaat ttacatttcc
      241 ggaaaattct acaatggctc ttccatttat aatatctact gcttgttttg ccatttccaa
      301 actcatatta tccaacatta ttatatctgc attagcttct attgcttctt ttaccatgtc
      361 taaagtttcc acttccactt ctatctttct aacaaaagaa gtattttttc tagctaattc
      421 aactgccttc ttaacactac cagcagcccc aatatggttg tctttaagca atattccatc
      481 agataaatta aatctatgat tgcatcctcc acctacttta acagaatact tatctaatat
      541 tctaagatta ggtatcgttt ttctactatc taataatttt gtatttgtac cttcaagctt
      601 ttcaacatac ctacttgtaa tagtagctat accactcatt ctctgtaaat aattcaatgc
      661 tacacgctct cctacaagta agtttctagt actacctgtt aatacagcta tcttttcacg
      721 attctttact ctatcaccat catttttata taattttaca tctacatcac caagtatctc
      781 aaaaactctc ttaaatacac ctaagccagc tattatacca tcttgtttac aaattaaatc
      841 tacagtagat ttagaatttt catccacaat agaattagtt gttatatctt cacttggaac
      901 atcttctatc aaagcatcct gtatcattct atcaataatc aaataattca tcgccaaaat
      961 cctcacttgt tctttcaatt tcttttatga ctaatatttt attttctttt tttacctgtt
     1021 ctatatctat atccatatct ggtacatcta taaacttaac ttttacatta tctataactg
     1081 agtttatatt tttagctgct ctttttgaaa atacaagccc ttcaagtaat gaattacttg
     1141 ccaatctatt ggctccatga acacctgtac aactaatttc accaactgca tataaatttt
     1201 ccatagatgt ttttgaatcc aaatctacat gtatcccgcc cataaaataa tgttgtgcag
     1261 gagatacctt tatacattct ttcgttatgt ctgttcctct agctaaacat tcattataaa
     1321 taaaagaaaa tctattaatt aaatattcac tatctaaatg tgttgcatct aaatacacat
     1381 aaggtaattt atctttttcc atttgctcaa aaatagcttt tgaaactaca tctctaggaa
     1441 gaagttcatc cacaaatctt tctttatttt tatttaaaag tttagctccc tctcctctta
     1501 atgattcaga tataagcatt ctcctacctt cattattttc ttcataaaag gctgtaggat
     1561 gtatttgaat atattctaaa ttttctattt ttatattatt tcttaaagct attgcaattc
     1621 catcacctgt aagatgtctt tgattagtag aatttttaaa aagcccacca attccacctg
     1681 tagctaaaac tactattttt gaaaaaacat gaatctcttt tttagattta aaaattctag
     1741 ctcctataca tttattgcca tcttttaata tatctaaaag ataggcatct tctataagag
     1801 ttatattttc tctagtttta gcttctttta ataaagtttt aaaaacaact tctccagtat
     1861 tatccttact atgaactatt cgatttacac tatgtgctcc ttctcttgtg taatctatct
     1921 ctccattttc tttgtctaaa ggcataccat attctactat ctgccctaca ttttctatag
     1981 attcatctgc aagtacttga actgcttcaa ctctattttt atactgccca gccttcattg
     2041 tatcttctac aaaggattct atatcatcaa tatttctagc agttgaaatt ccaccttgtg
     2101 caagatatgt attgttgttt tcaattgttg atttcgatac tactagcaca tttaaacttt
     2161 tatctaaatt taatgcacag tataatcctg aaactccact tccaactatt aaaacatctt
     2221 gttctaaatt cataagtcta tccctctatc cttctgctaa tttgtgcatg ttaagcagtg
     2281 aatttaaagc cttttccatg atttcatctt ctacaataac ttcattttcc atatttttaa
     2341 gtgtactata taaattttct aatgttgtct ttttcatatc tgtacatagt atttttccac
     2401 caggaatata aaagttttta ttaggattat ttttctttaa ttcatgtaat ataccttctt
     2461 cagttgctat tataaagtct ttgtttttac tattagtagc atattttatt attcctgatg
     2521 tactacctac ataatctcct aaatctctta tttctttttt acattctgga tgtactaata
     2581 cttctgcatt tttatactta tcttttaact caattatttc ttcagctctt atattgttat
     2641 ggtatttaca atatccgtcc caaaatataa agtttttatc tggaaattgt tctgcaatat
     2701 atcctcctaa atttttatct ggaaggaata taatttcttt attatctatg ttttctagta
     2761 ttttaattgc actagaagat gtaacagaaa catcacaatg tgccttaact ttagctgtag
     2821 agtttatata acatacaacc aaagcatttg gatactgttg ttttaatttt atcacgcctt
     2881 cttcatctac catatctgcc atagcacacc cagcgttact tactggcatc aatacagttt
     2941 tttcaggtga taatatctta gcactttctc ccataaacct tactccacaa aatacaacca
     3001 cttcttcttt acaatctcta gcaatctcac ttaaataata tgagtcacca actgcatctg
     3061 ctaattcttg tatttctggt ggttgataaa aatgggcaag tattattgca tttttttctt
     3121 tctttagctc tttgatttga tatgttaaat ctttatccat aattgcaccg ctttctatat
     3181 gtactagtgt atatacacct gtaaactcat tatatcactt gactattctt tcttcaagaa
     3241 ttttaaacct cttaatattg ttaagatata gacatttata ttctcaattg attttccttt
     3301 tttctccatc tatttaaact aatttttata ttttttaatt tttttgtaaa atattatcag
     3361 aattttgtct atattcttta ttgctataac attgaaaata ttaccaaata cctgtatact
     3421 aagtacatgg ttatgtatat gtatttattt ctgaaattat attagttgta atactttata
     3481 gaatttgtag gttgtccaga attaaattca atataaatct acacatttag ataatatgta
     3541 tacaaagacc aaaaaatttt ataaatgggg tgatattatg acaacattct taatcggctt
     3601 ggctatatta ctaataggag gtgctttata cggtgcttat tgtgaaaaag tatttggccc
     3661 agatgacaga aaaacacctg cacttgctca aagtgatgga gtagactatg tcccaatgaa
     3721 aaaatggaaa aatagtctaa ttgagttgct taatatagct ggtacaggac caattctagg
     3781 acctatccaa ggtatattgt ttggtccaat agcattcata ctaataccta ttggctgtgt
     3841 atttggagga gctttgcatg actatatgag tggaatgatt agtataagag aaaaaggagc
     3901 acaaatgcct tctcttatca gtagattttt aggtaataaa gtatttcaag tctataatat
     3961 attcctttgt cttttaatgc ttttagttgg tgcagttttt atttatacac ctggggattt
     4021 agttgttact caaattttaa atatgaaatc gactataaat aatccagttg tctggatagt
     4081 ttatggactt atctttctat attacttgtg tgcaacacta tttccaattg ataaaatcat
     4141 aggaaaggta tatccaatat ttggagctat tctactttta tctgctgctg gtgttggaat
     4201 tggaatcttt acacaaggtt atgatttagc taatttaagt cttgcaaatt ggaaaggtat
     4261 tcatcctgat ggtattcctc ttattcctac attctttgtt acagttgctt gtggtattgt
     4321 ttctggattc cactcaactc aagctactct tatagcaaga tcagtttcaa atgaaaaaga
     4381 aggaaaaact actttttata atatgatgat tttagaaggt ttaattgcaa tgatttgggc
     4441 tgcagctgct atgggtattt acaataaagg aatacctaag gaattagtag gttctcctga
     4501 tgtaataggt ttggttgcaa gagatttact tggttctatt ggtggtatca tagctataat
     4561 aggtgtaatt gttttaccaa taacttctgg agatacagct ttaagatctt taagattaat
     4621 gcttgcagat tactttcatt atgaccaaaa ggaaaaaaaa catcgtgtta tattgtcaat
     4681 ctgtatattt attcctgtca tagccatcct tatatttgca aagcttagtg cttctggatt
     4741 taatatattg tggagatact tctcttggag taatcaaact attgccatat ttgcatttgc
     4801 aatgattaca gtttacctta taataaaaga aaagaattat attataagct taattcctgg
     4861 tatgttttat tcctttgtta tattcagtta tatatttaat gctcaaattg gatttaactt
     4921 aaacataaat atatcatatg ttttagctgc catatttact gtattatatg ctattttaac
     4981 tgtacgctct ggtagaaagt taaaaagcaa agcagatact aagttggctg actaaatcta
     5041 aataaaataa tacttagaat aaattttatt aaaaaatgat aaaaaagtag gcaatctcaa
     5101 gttagatttg cctctttttt atttaataca gtaataaata cttaagtatt tactccttat
     5161 tggaatattt atcctaaatc tctaatataa ataaataaaa gttttaaaat aatctattcg
     5221 tttatatact taaaataaat tttttggagg aatacttatg aataataaat tacatgaact
     5281 tgaaaaaaat ctaccagaaa catcttgttc tttttgcaca catctttcat ttaaggggcc
     5341 tagcctagac tatagatatg atattagatg tgttatttca gatacaaagc ccgattttag
     5401 aggatgttgt gaatattttg aaccagaata tacagaatta aatacaggag atttagacaa
     5461 tctatacata aactttcttg agacatgcct caaggtaaat tataatgatt acatcaattc
     5521 tatgtattgg aagttattta aagaaagaac cctactagaa aacaatttta aatgttctat
     5581 atgtggatca actgaaaatg ttgatgtgta tcatgttaat aaaaatttag gaagggaaac
     5641 ttccaaagat gtagttgtta aatgtgataa atgtaattgt aaataagtgt ataaaattta
     5701 gataacttca cataataagt ttaaataaaa cttaggaggt tatttatgaa aaataataat
     5761 ttaaattgtg ctgctactaa ttgtgcttat aatactagtg gatattgtta tgctggtagt
     5821 ataaaagtag atggaatgca agcaactact acagaaaata cttattgtgc ttcttttgaa
     5881 gacaaatata catctggtat aactagtcgt tctaatgaaa ctaaccaagt tgatacagat
     5941 aacattcatt gtgaagctgt aaaatgtaaa tacaataaaa atgaactttg caaagctgaa
     6001 aaagttcata taaatgaaag aaatgcaagt tgtgaaactt ttgaaatgaa ataaatttct
     6061 ttttatttag ttttatagta tttatacaaa gcaaaattga acctatcaaa tagatgtctt
     6121 tattaatgat gtctatttat aggttctttt ttaaaacaat ttataattta tatataaaga
     6181 taaattatta ttttaatata ttacctagaa gtaaatttaa cacccagaat atccataagg
     6241 aacataaata ttggaactcc aattagtaat ccccatacac ctaaataatg ttctcctaca
     6301 agtaaaataa taaaaacaaa acaaactgga agtttagttc tattcgacat caacttaggg
     6361 tttaatatgt atgtttctac tgcatgcact atacaaatca taagtaaaac tccaaaaacc
     6421 tttgttattc ctcctgtatt aaatgctatt acagttaatg gaatcaaaga cactataact
     6481 cctgctactg gaataagacc caacacaaaa atcataaatc ctaaagccca tatttgaggg
     6541 aatcccatga aacctaagaa aatcatagaa acaacagaat ttataaaggc aatcataact
     6601 tgtactttca ttacttcacc aaaattttta acaaagctct ttccaaaaaa tacaagcgaa
     6661 ttataaataa aagaaatctt actatcagaa agtttatctc caaaattttt aatttcattt
     6721 ttttcaagta aaaggagtaa actcagtaaa aatgccaata atatatttac tccaaaactt
     6781 ccaacttttg ttgctgttga tgctataagt agccctgctt gtgaaatata tttattaaaa
     6841 tccagctttg agacaatagg atacaatttt ggtcctaaag attgagcaaa ttgatttaca
     6901 tcaaaatcac taaaaaccct actcaactct gtaagttgaa ctataattat tggtacaact
     6961 gcatagctaa tcaatactaa caatatagca aatagtgcat ataacactat aattattaat
     7021 gaatctggaa tatttaaaga agaaaacttc ttaattcgtt ttcttgtggc tacaagcaag
     7081 ttatagaaaa taaaagtcat aataaatgtt aaaagcacca tatttaacat ataccaaaat
     7141 gtaattagaa taattactac gacagatata gaaacaattt tatattttga actgtcacgt
     7201 gccaatcctt tgttatcttc catagtttaa atcctcccca aatttttatg actttataat
     7261 gactgccctt tttattttaa tctcaaaaaa caagtagaaa aatactaaaa ataacttatt
     7321 aattttcata aaacaatatt aaaatttaga gaagatacat tttaaataat cttacttata
     7381 ctgaaatctg ctctacatca tatatttaat ttattattat ctatctcata tagcaattcg
     7441 tatatctctg gatattcaaa aaacaaaccc tcttttagta attgtcttat ttcatctgtg
     7501 cttacccact ttatatcact tacttcttcc tcctgtaaaa tagctttgga tatatcatat
     7561 tcttttttaa ctaagtatac atcccaaaga gtatcttcat gaatcatact tctaaaaact
     7621 ttcatctcat ctttagttat atctatccct atttcttcct ttgcttcgcg aattgctcct
     7681 tctaaactgt cttccccaga aacaatacat ccagttgtca ttccccacat atttggcaat
     7741 gtctttttac ttttagacct tttctgaatt aaaatttgag agttactatt taaaatccac
     7801 acttcaactg ccaaatggta ataaccttct tcaatagaat tacctctttt tataacattt
     7861 cctgttttaa ttccatcagc attatataaa tcccataatt ccatttcaaa atctccttga
     7921 ctttactata aattagataa actacaaata tattttagca taattaattc tgctcatttt
     7981 ttattacaat acttgattta catttatgtt cattgctttt cttttccttc cattctctta
     8041 tatcatctat aataaatcta tgtttcaaca attttgtgtc aaatttaaat attctaaaca
     8101 ctatttgtaa tacatatcct aaacctaaag cacttacaag cgtaccaata cctacagaac
     8161 ctcctaataa ccacccaaca actaatgcta gtatttcaag aattgttcta acaagacgta
     8221 cagatttatt agtttttttc tgaagagcaa tcataagccc atctctcggt ccacttccta
     8281 aacaaacacc taagtataga aaacttgcta tagctgccaa aataattcct ataacaacca
     8341 taaacacacc tatataagtg ttgcttgcat gaggtatcac tccagaaaat attactaaat
     8401 caataaaaat tcctataaat gtcatgttga gtaaagtacc ccatcctata ttttcaccaa
     8461 aaactgcatc caatataaca attacaactc ctaccattat agaagcctga cctatagtta
     8521 tgcctagctt tatagataaa ccttggtgaa gtacatccca aggcataaga cctacatgtg
     8581 cattaatcat aagcacagaa gaaattgcaa atataaaaaa acctgaaaat aatcttatca
     8641 tactttttaa acttattttt cccatcaaaa atcactcctc ataataataa taacaataaa
     8701 taccaataaa atatatatat aaatacgaaa ttttacagaa ttatttatta ggttaagata
     8761 aaattttaat tataaaaagt ggcaatccaa attttataca ttttcaccta tactctaagc
     8821 tttcgtgtat aaaaaacgga tataccactt ttttagtata taaacatttt tagtttttct
     8881 tattaatttt cactataaat tctagcttct tcttctccat ttagtactct taaagttcca
     8941 aaagcaagtg attccaactc attttctcct gccataattt ctactggtgc tataaactca
     9001 actttctttt taaccattga agtaaacatt tcagaatatg caattccacc tgttataatt
     9061 atagcatcta cctttccatc tactacagtt gctaactctc caataccttt tgctaactga
     9121 taagccattg cttcataaat aatttttgct tcttcatttc cttctgctat cattttttca
     9181 acttctctag catctacagt atttaggtat gaaactatac cacctttacc tcttatcttt
     9241 ttagtcattt ctctttctga atattgacct gaataacagg cagccactaa ttttgtagca
     9301 ggtactcttc ctgacctttc tggagaaaat ggtccttcat catcagatag catatcaacc
     9361 attcttcctt tttcatgaag ataaatactt actcctccac caatgtgagc tactattaag
     9421 tttaagctct tataatcttt tcctttatcc ttagcatatt ttaaagccat tgctctagta
     9481 tttaatgcat gccctgcact tgatctatcc attccacaaa gtccagatat tctcgctaca
     9541 tctataagct catctactgc aacagaatca taaatatatg agttgattcc aagaggttct
     9601 gatattgcat gtgccacaac agcacctaaa ttggaagcgt gttcaagtac tggtctatgt
     9661 cttagtacat caatcatttc ttcatttact aaatatgctc ctgattttac tggtggtaaa
     9721 actccacctc ttcctactat tgcactaaga gattttaagt ctatactatt ttctttaaga
     9781 atattcaaaa ctgcttcctt acgcatttca aactggtctt gtatagtatt atattttgca
     9841 atctcttcag ctggatggtc tatcgtcttc acaagaattt gttcttctcc atcatatact
     9901 gctatttttg tagaagtaga acctggattt atggctaata ttctgtaagt cataattttc
     9961 ttcctttcac cttaatttct atagtctatt tatatctatt ataaactatt tgctaaccat
    10021 ttccatacct tcatggaaag cttttatgtt taattcaaca aattttggtt taacattttc
    10081 acttataatt ttttcccaat caatagactc taattgcata gatttaacta gacatcctaa
    10141 taaaataaca ttcattattt ttgaatttcc tagttcttca gctttatcag ctgcatttat
    10201 aacttttgca tttaatctac ttaactctgc atctatatca ctttcttgat attcagctcc
    10261 tccgattaaa acagtcatag aatctatcct atgattattc actaaagctt ttccttctgg
    10321 ttttaaataa ttgaaccatc ttaaagcttc cattttttca aaagatacta atatatctgc
    10381 tccaccaatt tctataactg gagaatgaac acaatctcca tatctaactt gtgaggaaac
    10441 tgaacctcct ctttgactca ttccatgtat ttcactcatc tttacatcat atccagcttc
    10501 catcaatccc atagttaata atttactagc aagtatagtt ccttgacctc ctacacctac
    10561 caaaagtata ctcttagtca tattattttt cctccttaac tattgcttgt tttggacaaa
    10621 cttgtgcaca tactccacat cctacacact gatttctatc tatatttgat aacttattct
    10681 ctttatcaaa tgatagagct ggacatccag ttttcaaaca taatttacat cctatacatt
    10741 tatcatggtc aactttacac ttagtcttaa atggattatt aaattcttct atatcttctt
    10801 tagagaattt tttaagtaca catggccatc ttgttattat tactgatggt tcatcttcta
    10861 tagctaaagc ccaatctagt gcttcattta cttcttttaa attattaggg tttataactc
    10921 ttacatgttc tattccacaa gctttaacta atccttctat atcaacttct ttagttttag
    10981 caccttgtaa agtatatcct gaacctgggt tttcttggtg acctgtcatt ccagttattc
    11041 tattatctaa tataaccgaa atagaattac ctctattgta aacaacatct aaaagtccat
    11101 ttattcctgt atggaagaat gttgagtctc caagtacacc taccaatctt ttttcattat
    11161 catccttcat attaagaact ttttgagctc catgtgcagt tccaaaagca gaacccatac
    11221 aaacaacata atctattcca ttatatggag gagcaaatcc aagagtataa caacctatat
    11281 ctccaccaac tattaaattt tttctctttc caagttcata gaagaatcct ctatgtggac
    11341 atcctgcaca gaaacttggt ggtcttggtg ttactaattc agatttgtat tctatagtat
    11401 catttgtttt tccaaatata gcttttctta aaacatctgg tgtcatttct ccatatggtg
    11461 gtattacatc ttttccaata caatctattc catatgcttt tatttgttct tctataaatg
    11521 ggtcattttc ttctataaca tatattttat caactttttc tgcaaattct tttatttttt
    11581 cataaggtaa tggatttgta aatcctagtt tcatatatga tgcatttttt ccaaaaactt
    11641 ctttggcaaa attacaacac ataccagatg ctataacacc tatttttgta tcatttattt
    11701 catagtagtt taaatcagtt tcatttgaga attttttaag aatttccatt ctttcttcaa
    11761 caacgcccct tcttatttga gcatttgctg gaacagttac cattttctta gcatttttaa
    11821 catactcttt aatttcaact tcttctctat catagcactc cactaatcct tttgaatgac
    11881 aaagtcttgt tgtaactcta tataaaactg gtgtatcgta tttttcacta acttcaaaag
    11941 cttcttttat catatcttta gcttcttgac tagttgatgg ttcaaataaa ggtatctttg
    12001 caaattttgc atacatccta ttgtcttgct cattttgaga tgaatgcatt cccggctcat
    12061 ctgctgtaat aagtaccata ccaccattta cccctgtata tgcataagta aatattggat
    12121 cagctgcaac attcaaacca acatgtttca tagaagccat tgttcttgct cctgcaattg
    12181 aaccccctat agcagcctca agtgctacct tttcgtttgg agcccactct gcaactattg
    12241 catctttata tgttgctata ttttctaata tttctgtact tggagtccct ggatatgcag
    12301 atgcatattt aacaccagcc tcataggctc cacgtgcaat tgcttcatta cctgtcatca
    12361 attgtttcat ataaaattcc ctccatctac aattattaaa ttcatagtcc acccaatttg
    12421 ttcaatgaaa ttaattattt atttttactt ctaaactttc aagtgcttct tttataatac
    12481 ttggtgcttt cttacattta tcttcagaaa ctgcacaaac tgcaaatctt aagcccattt
    12541 ttaatggaac tgcaaataaa ttatgtttag tcaattcttc acatacttct tttggattat
    12601 cacatggtat acttacaaag aaacctgcta tatatggaag aatttctaat cctactcttt
    12661 cagcttcttt tacaaataca tctgctcttc tagtaagcat gtttttgtat atttcttttt
    12721 catcctcata ttctttgaat tttttagggt catttacaat gttagataat actgccatag
    12781 cacttctatt acaatttgac caatttgctc tacatgaatg aacacatgag taatggaact
    12841 cttcagctac atcttcactt gaagatatac aaattatagc tcccattctc ataccataag
    12901 ctgtaaatcc ttttgacata ctgaatccaa ccaatacgag aacgttttca ggcaaattag
    12961 aaaatttctt aaaaaacttt ctacatacat catcatcacc tgcaaagtca atatatgctg
    13021 aatctacgaa aaatattatc tttttgtctt tatcttttgc gacttctttt gataagtcta
    13081 atattttatc ccactcatca tctgcaacac tatatccagt tggattatgc gctggtgtgt
    13141 ttataattgt aaagactcta ccttgttttt ctgctatatt tacaaatttt tctttaaacg
    13201 actcaaaatt aaaccttcta ttttcatcaa aaagttggta attaactact tttctattag
    13261 cctcttcagc tatacttaca tatggactcc agaaccaatc agatgttaat acctcatctc
    13321 cttcattagt atagttccat acagctaatt ttattgcacc acttcctcct ggtgaagcta
    13381 atactcttat atgtccttct ggtaagtaat ctctgaaaaa tactttttta actgcttcta
    13441 agtaatctgg ttgaccttca agagctgcat atgcacctat ctcacagtta tctaatgctt
    13501 tatattcttc atatacagtt ttcattgtta tcaattttcc tgaatcatcc attagagcgc
    13561 ctattgttgc atttattaca ttatccatac caaaagcctt ctctgctgct tgagctctgc
    13621 cagatatgct aaatataaca tcattttctt ttggccacat tgcatgctta ccaaccatac
    13681 tttgactcat accatcaccc ctaaaattgt atttactctt acattatata ttaagcaatt
    13741 actatgccaa taaatcccac tttatgtaaa attgattttg tttagaatta ttattttttt
    13801 cattattaaa ttaagtgttt taactatttt tcttataaat atatatgcct gtctaaaaaa
    13861 ataaaaattt gcaaaaaagt aatgttaatt tttcttaaca cttgttttaa aaaattaaca
    13921 ttacttcaaa ataatttata tgtaattata tttaaatatt tttattgtat ttcttccata
    13981 aagatgtcct gcttattcca agaacttgtg caacttcatt tttagaaagt ccctgagcaa
    14041 aaagcatact tattattttt tcttcaacat acttatttac ttcttttata tctatactta
    14101 tcttaccatc atttatctta cttatattta tagattcatc ttggacttct acacttgctg
    14161 tcgtttcaga attaaatttg tctacttcag agttaggaag taatttttta gttttaaaat
    14221 ctctatcttg tctcaattta attttacctg tcacaacata tttttgagct attgtcttta
    14281 actctcttac atttccaggc cagctataat tatatagctt atccatttca tctttagtca
    14341 aaacaaaatt ttcatctata ctgtttattc cattgagacc atcatccttt aatacttcat
    14401 ttacaaaatt gttaaacaaa ggtattatat cttctcttct gtctcttaaa ggaggtatat
    14461 ttatctctaa actactcaat ctataaaata agtctgctct aaatgtaccc ataactattt
    14521 tttcggttaa agattcattc gttgctgcta taatccttat atccagaggt attatataat
    14581 cagaaccaat tctcattatt tgacgttctt ctattactct taatagtttt gtttgaatat
    14641 taaaggatag actatttatt tcatctaaaa aaagagtccc tccatgagca agttcaaata
    14701 gtcctctttt tccacccttt ctagcaccag taaatgcacc ttcttcatag ccaaaaagtt
    14761 cactttcaag taaattttct gctatagtag cacaatttat agcaacaaaa ggcctatctt
    14821 ttcttttact tatgttgtgt atactatgag ctataatttc tttaccagac ccactttctc
    14881 cataaagtaa agttgtataa tcactttttc caattttttt agcttcttca ataaattctt
    14941 tagttaattt atctttaaat aaaaaatcat caaaagtata tctagcatag agaccttttt
    15001 gattcaagtc aaatcttatt tttctttcta agttttgtag cttggttata tcttgtatta
    15061 ttcctaaaac tccataagta ctattatcaa cttttatcaa tgttgtccta gtattaacaa
    15121 ttagattatt tatatttctt atctttctat cttctacgtc ctctttttca tgtaaacaat
    15181 ctaacatcca ctccatttta ggaaatacat ctaagatgta tttattaagt gcacattttc
    15241 tctctacttt aagcatgttt tttgcacttt cattgtacaa aattatactg ccattactat
    15301 ctatagcaat tactccatct tttataccat caagtatatt tcgtaacact tcattattaa
    15361 acttttcttc acctaatgta tctaataatt ttttacaata ttctacagct tctcttatag
    15421 attcatcact agcagttgca aatacactat caattccata ttgccttgca aaagaacatg
    15481 ccaaacctcc tcctacgatt acaacttcat ctttttggtc tatatatttt acaactttac
    15541 ttcttatctc atacttagat tcaaaccatt cttcagttat ttcagtactt atgacatttc
    15601 tccagccaac atagtcaaaa gaaacttcat tgtctccaag aatcaatact acttttttag
    15661 aatatttctt tgctatttct attgctctta aaaggtcagt tgacttcata ttcattggta
    15721 taacaggtac atttactgta tctattacta agctataacc tccacctcta ccaattatag
    15781 cttgggcacc tttttcaaca agtatacgac cttgattttc cattaagtct aggttaagta
    15841 tgtcgattat tatagttcca ttttctacat cttctctata taattcttct attctttctt
    15901 ttaattcaat atcagaggct ataattccta ttttttttcg caacaaaatc acctcaattt
    15961 taaattataa tgtattgatt tctacctgct ttttttgatt tatataggtt cttatctgca
    16021 tcttcaatta atatttcaac atcttttcta ctacgacacc tttttatact tactccagca
    16081 cttatagtta tatagttttt tattttagaa tatatatgtt caataccctc aaattcaaga
    16141 agtttgaaaa ctcttttaat atataattct atattatcaa tctcttcaac accgattgat
    16201 tctatagaat ctactaatat taaaatttct tctcccccat atctgattat gtgttcggta
    16261 atatctataa atacacttct tataatatca gttaccttaa ctataacttt atcccctgct
    16321 agatgaccat aattatcatt ataactttta aaacaatcta tatcgaccag tattgcgacc
    16381 atatttttgt ctctggttcc attaaataaa tccaactgct gttccaaata tcttctatta
    16441 tatagtttag taagtccatc ttttaaaata tcattttcta atttcttatt atcatccact
    16501 agtaatctta aactctcttg tttttgtctg agttcatatt gtttgtgaat tgcttgagat
    16561 gttttatagg aattattttt ttcccaccta tcagaatacc aatccatact tttataatat
    16621 tctattgcca gtttaaaatc tttttttagc tcatatccat ttgctattag cccatataaa
    16681 tatagatatt tttctaagat tcaatgaact ctttttaact aaatttaaac cttttatata
    16741 acaacttata ccttcatcta tattaccaaa ttttatatat aaatctccat acaacttata
    16801 tatgtaatct agtttagaat tatatataaa aacatctcca aaacttttat atatattttt
    16861 tgataaaatt aaataggtgt ttaccttttc aaaactacaa ttatctttct ttaactcaat
    16921 ttcagctgaa tataaaaata taaatgaata taaataatcc ttatatacac cttcaacaac
    16981 tttcattaat tttatagtct ttaataaaat aacttctgct tctttattca tatcttcttt
    17041 taaatatgcc catataaggt ctatttttat agacactctt tcagttactg atattgagtt
    17101 attactgtgt gcaacatcta atacattatt tatgtatttt atagcttcta cattatttcc
    17161 actctctaaa taacacaaac ttattataca tttacaaaaa gcctctatag agtataaatt
    17221 attttcttga gccaatttta gacattcaat taagtattcc atacttttta taaagttatt
    17281 aaataaatat agataaactc tcccaaatat ataatttgcc ctacttacaa gagctttatt
    17341 ttttaaagtt ttaaaaaaag acacttcttc tactaaagct attacatccg ttttggtctg
    17401 atataaagca cttatttcca cacataaact catatttaac aataccaaca actcatttaa
    17461 atgattttgc cttataattt tttttgctga gaaataaaaa ttatttacct tatcatattt
    17521 tcttatagaa atagctgata aagtaagata aatataaatt ttagatatta tatctgaatc
    17581 atcttttaat gacaattttt taatatactt taatgactct atcaaatagt tgtatgatgc
    17641 ttctctatct ttttttatat agtttataag actaattaaa tataaagtat agactctact
    17701 attatcttct aattttgtat ttaaaaggtc tttgtgataa tccaagatgt agtttaaatc
    17761 tgtcaaattt agatagaatt tagtcaacat atcgtattct atcttgttta tatttctctt
    17821 taaatcttct attaaactcc ttttcaagta aatcaccaat cctatttcaa cagttaaaaa
    17881 ttcttaaaat catgataatg caaacatcta tttctaccac tttcctttgc tttatataaa
    17941 gctttatcag cattttcaat tatttctttt atatctatac tactattttt acatgtatca
    18001 atgccaatac taactgtcaa attattattt aaaaatttct ttacaatcct acataatttt
    18061 cttccaaata ttttactttt tttataatct ttacatacag ataatatcag aaattcttct
    18121 ccaccatacc ttatcataag catgtctttt tttagatatt tttttattat tatagttata
    18181 tttttaagag caatatctcc attataatgt ccataagtat cattgtattc tttaaagtaa
    18241 tctacatcta tcataaatgc tgtgcaatat tcactaactt cttgtttttc taaaaaatcg
    18301 ttcaaaaaag ttctattata tgcacttgtc aaatggtcaa catacttttt tttatctaaa
    18361 tcaagtttta cttttctcat gttatttatt tctttttcta tatactcatg tctataattt
    18421 aaggaatcta tataatttct atttcgagct tttatataca tcatttttaa ctcataatac
    18481 attttaaagt atttcatact catttcataa ttttgaatct tttcaaaaca caaatatata
    18541 ctcttgtatg ctaacttcac ataattataa tttttagcat ttttagattt ttcaaggagt
    18601 tttttatttg tatttaaggc attgtcataa tatccaaaga tataatatat ctttgcctgg
    18661 tgataaatta tttctaaatc atatattata tattcttcta tattttttat catatttaat
    18721 atgtttttag ctctatctaa atagcatatt gattgttcaa aagatttttc attacttttt
    18781 gcataataca tggctaaact tataaataaa tcaaacttct ctacttcttt taagctatga
    18841 tttttaattt tttctagata tacttcagat tttgctaaat tatttattgc gacaggataa
    18901 tttctagtct ttattaatat tttagttatg ttgtttgatg ctctatattt ttgaattata
    18961 tttacatttc tatacttttt attgcttatt acttcattga aatattccat ggaacattca
    19021 taattttcaa acgaacattc acactcacct atctttatat tacatagtga agatacttgc
    19081 atatccttta gtaaatctgc atattttctt gcttttaaaa aatttacctt agcaattaga
    19141 gcatcatcca aaaacaaaaa atatatttta ccaataataa aaaaatatat ttttttacaa
    19201 ttaaaatcgt taatttcttt tatctttata tacaacgttt ctatttcttt aattattaca
    19261 gaagattcta tacacttact tgccttaatg taaataagtt gtgatttaaa aataatttct
    19321 tcaattagaa aattattttc atcacaaata agattaaatt tatccatata taaatttgcc
    19381 ttatttacat cttcattttt taaacttaaa atagctattt gtgaataaaa tcgtgctaaa
    19441 aatttataat ctttgggtaa gcatttatca atatatttac aggaaatcaa aatatactct
    19501 ttcatttttt cactaaaccc ttgaatacca tataataaag ctagcaaaaa atttgatttt
    19561 aataacatta aactattttg ttcagtttta tagcaatcaa ttaaattctt tactacatca
    19621 attttatttt caataaaatc gttattagtc aataatcttt cattcaaggt tagtttaaac
    19681 attgaatcta aaattgtatc atctatctct tccaaatatt tactatatat tgtaactcac
    19741 ctccgactta cattatctca taaatttagt aattttccat actatttttg aaaaaagttg
    19801 atgtctcaat ttagaaattt ctcttaaatt aagacatact ttacactaaa ctatacaatt
    19861 ttctatctga actatataat cctgacaatt ttcgcaaatt atcacactta caatataact
    19921 attatcaact ttatttgccc ttgatagttt attaaaagat agtcctttat aatcaacaga
    19981 tatattgctt attacatatt ctccatcttt actgaaaata taaaagtgat attctaaaaa
    20041 aggaaagaat tttttctttt cttttactcc ttctaaacat aatggacatg cttcttgata
    20101 atattctaca tcaaaatcaa cttcttctgc ttcattaagt atatcaatta tttttgaaat
    20161 acttactcct tgaactggat tttcttcatc atttattaaa gcactacagt tctcctcttt
    20221 taataaatat tccttatcat catatataaa cttatataaa ctcatattat atcctcctta
    20281 taattattaa tctccaagtt attctaccat ttaaataaag ttaataaaag taattgttta
    20341 taatctttat aattaagtta tgtatacata tattcctatg cgctatctta attaatgatt
    20401 ttaaaatcca agtttgcact ttgaacatcc agtttcccaa aatctattgt caaaactcac
    20461 tttagttatt aatataatga tataaatata taactgcata gatgttacac acttaactat
    20521 ttaatatgat acgatttggt atctaaattt atatattgta tataggggga gatatatatg
    20581 tataaagata aaagtgatga atgtatacac ttaatgactg catacatcga tagtataagt
    20641 ggatattatt catttattga cacacaatta gaggatttta tgatgaaata tggtgaaaac
    20701 attgttgatt caaatttaca cagtataatg atgttgcttt gtaaatgggg attatcttag
    20761 catttcaaga aacttttttg tatattgtat tctatattat ttattcatta acaaaaaaga
    20821 ttgacataaa cttaaaagct atatatagat ttctttaaaa atctatatat agctttttta
    20881 attttttcaa aatgtatact taaataagtt agtttttttc aaaatttgac tatctaatat
    20941 gatataattt aactatctac aacaataaaa aatatctaat atgtattatc tagtttagct
    21001 catcataata acatccctat atttacaatt aagatatttt caatatagta atactcaatt
    21061 ttaatattat aaaaaggaga gattttgtat ggattacaat gacttactaa aatcagctag
    21121 agagaacttt aatggaacat gtaaagtatg taaaatttgt aatggattag cttgtgctgg
    21181 agatgttcct ggtatgggtg gtaaaggaag tggttcgtct ttcattgaaa atagaaaaag
    21241 tctagaaaaa ataaagataa atatgagagt catacataat gtttcaaagc cagatacatc
    21301 catagaatta tttggtagaa aaatgagttc tcctatattc gctgcacctg tatcaggtac
    21361 tatattgaac atgggtggta aagtttccga aaaagaatac atagagcctg ttgttagagg
    21421 ttgttctaat tctggaattt atgctatggt tggcgatact aatgtagata cttttttact
    21481 ggataattta gatgtattga aggataatag gggaaatgga attgtattta taaagccttg
    21541 gaataattca aagataatag ataaaattag attgtctgaa gaagcaggtg cttttgcagt
    21601 tggtgtagac cttgatgctt gtggacttat taataatcaa tttcaagaaa atcctttttc
    21661 tcctaaaaca attgatgaga ttagagaatt ggttgaatcc actagattac cttttattat
    21721 aaaaggaatt atgactgtag atgatgcttt gatggctgta gagtctggtg ctagtgccat
    21781 tattgtttca aatcatggtg gaagagtact tgattatact cctggtactt gtgaggtact
    21841 tccagatata gctaagtctg taaagggaaa gataacaata cttgttgatg gtggagttag
    21901 gactggtgtt gatgtagtta agatgcttgg attgggtgct gatgctgttt tgatgggaag
    21961 accttttgtt actgcttctt ttggcggtgg tcttgatggt gtggagtttt ttatagagaa
    22021 ggttaggaat gagttatgtg agactatgat tttgactggt tgtcaaaatg ttaaggatat
    22081 agatggtaga gttatatgga atttattgta aaatagtttt ataacattta tatttaaata
    22141 gtgtctcatt taaattgaaa aatcatattg agacactatt taatatattt ttagatttta
    22201 atcctatgaa agagtagggt cagttagtct acccaattta tcccaagtag ctggaccaat
    22261 tattccatct gcagacaaac cgtatttttt ttggaaactt acaactgcat tatatgtctt
    22321 acttccaaaa gaactatcta tacctacatt ataaccaagt aaagttaaca tgatttgtgc
    22381 agcttttact tcttttccgc tattaccctt tcgtataact tttgttgtac ttatagtcaa
    22441 atactcatta ctcataattg ttatagtttt agcacctata ttataagaaa cagtataact
    22501 tgcataaaca cctttactat tgtcctcatt cattagatta gctgattctg gaacaaatat
    22561 ctcatcatct ggcttagggt ctatatttat ctcttcacaa ttttgtttat gttcatctct
    22621 tatttcttgt ggcgcatttt catatgtata tatataccca gaaagagtaa aagaatctag
    22681 gtttttctct ggaacagtct ctttacttcc tcctttctct ttacttgtgt cagcaaatgc
    22741 cacacttgta aaagtaagca ataagcaaca cataatagct actatttttc ttaattttgt
    22801 taacataaaa tcctccttat aattatttta ttaattgatt ataatctttc atatccattt
    22861 ctattatgtc atagtctcta ggtattttac cttctaattc gcatttatct tcataaaatt
    22921 tcctaatttc atttggtgca tctttataat aaatatttat aattttatca gtcgtcttat
    22981 tcaataaaaa ttcattaata ttgttattca ttccattatt accctcatca attctaactt
    23041 gagcatcaac ttcaacaaaa gatgttatag aagtcataaa tactaaaaca gcaattagta
    23101 ttccactttt gcctttcttt ttaacaatac tctcaagtct cttttccaaa tttgtcttat
    23161 caaactcggt tgtcaaaatc ttactattat aatttttact aaacttaatt gactttataa
    23221 gtgctagtgc atattctttt ttatctttta aagaacattc taccaaaaca ctttcatcac
    23281 aacataattc acaatctaga tttactttat tgctcattac atatacaagt ggattaaacc
    23341 aatatattat tctaacagac aaaactaaaa actttattaa aatatcttta tttttaaaat
    23401 gcattaattc atgtttcaaa atccaagtta gttcatcttt actataagga tagtctggta
    23461 agaatatgta agatttaaat aatcccatac ctgctggact tatcaattca tcagaacctc
    23521 ttaattcaat tttctttttt atatttaatt cttttaacaa gtttttgtag atacagttta
    23581 tatcattatc ttcaatatca taagatacat caacaactag atttttaaat cttgtatact
    23641 ttataaatgt ataaactgca atgactatag ttacaaataa ccataaataa gctacataca
    23701 gcataagatt attatctgta gaaatatcaa tttgtgtaat attatttcct aatgcatttt
    23761 ctttgggttc ttttacttca tacactattg tataattaaa tagaaaaagt aacattctaa
    23821 agactattat caaccaaata taataattga atctcttact aaacttctta aataaatatc
    23881 ttttcaaaaa taataataga caaataccaa gactacatac aatagttgtc tttataagat
    23941 tttcaaaaac atatagcata taattattca tcctttaaat tatcaaaata actttctaga
    24001 aaatctagct tatctttacc tatattttca tcatcatgta atgctgaaat taagcttctt
    24061 aaagagtcat catgtatatt acttaggaaa tcctttgtct caaaacttaa ataatctttg
    24121 tgttttactc ttattgtgta atgtgtatat ctgtctattt tttgagcatc taagaatcct
    24181 ctttttacca gcctagataa aagtgtcagt gtggtggttt gtttccagcc atatttttgt
    24241 tccatagctt caataacatc ttttgatgtc actgtaacat ctactttcca aatgaatttc
    24301 attactttta gttctgcttg tggtattttt cttatcatta tttacacctc cctcaaaact
    24361 cctgctacct taattctaca acatgtgaat tttttagtca aacactttat gaaaaaaatg
    24421 tgaatttttg tactttttgt taaatttcta atttttttac aatattaaaa gttagatttt
    24481 aaaatatatc tatttaaata ttttactttt tacatattta attacaatag tgtaga
//
LOCUS       DAMJVK010000055        24493 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.056, whole genome shotgun sequence.
ACCESSION   DAMJVK010000055 DAMJVK010000000
VERSION     DAMJVK010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24493
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.056"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            39..155
                     /gene="rrf"
                     /locus_tag="PC414_002303"
     rRNA            39..155
                     /gene="rrf"
                     /locus_tag="PC414_002303"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            275..466
                     /locus_tag="PC414_002304"
     CDS             275..466
                     /locus_tag="PC414_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HDF2464695.1"
                     /translation="MKKVALILAIIGALNWGAIGILGTDLIGSIFGGTYEMVSRIIYF
                     IVGLAGLYLIPSLMSDDRE"
     gene            621..2030
                     /locus_tag="PC414_002305"
     CDS             621..2030
                     /locus_tag="PC414_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF2464696.1"
                     /translation="MFIINELNKIVNEEVISFHMPGHKKGQVYKKLGYKDILKNLYKM
                     DTTEILGTDNLHFPEGIIKNSQKKASRVFKSDYTCYLVNGSSCGIQSAIMSVCSPKSK
                     IITNRDCHQSVINGCILGDIDIEYVPCEISEETNVLKGISISNVIDIIDKNLDAKAVL
                     LTYPTYYGMTYDLEYICNYAHSKKMVVIVDEAHGAHLGLSERLPKTALEQGADIVVQS
                     THKTLPAFTQSSMIHIQGDRVDIDKILNMLRITESSSPSYLLLSSLELAVDIYENKGK
                     ELMEELLNNIITFKNKVDSNINIYDTNDKTKIFISSKNMGLTGYELENILRNKYNIQV
                     ELSNYYGVLLICTIGNTVEDFSSLETALNNIVKEDFKFEKLENKKYPVVIPKKILTPR
                     EAFYKTKKSVKIYDSIGKVCGECIVPYPPGISVISPGEVISKEIIDYLMFCHSKGMIV
                     SGLKDVDLNFIQIIDLEDI"
     gene            2161..2850
                     /locus_tag="PC414_002306"
     CDS             2161..2850
                     /locus_tag="PC414_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HDF2464697.1"
                     /translation="MKGKLIIIESGSDASGKATQTEILYERLKKEGYMIKKVEYPNYE
                     SPSSALVKMYLKGDFGKNASDVDPYIASTFFTADRYASFKTDWEEFYNEGGIIIADRY
                     TTSNMVHQASKMDICDREEYLNWLADYEFNLFKIPQPDCVIFLDVPIEFSKKLMENRK
                     NKITGEEKKDIHESDIEYLEKSYNNALYIADKYDWKKINCVLDNKLRSIESIHNEIYK
                     IVLDSIKPMER"
     gene            2853..3788
                     /locus_tag="PC414_002307"
     CDS             2853..3788
                     /locus_tag="PC414_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HDF2464698.1"
                     /translation="MYFENIIGQDFAKKYLTNSIKKNKLNNAYMFEGMDGIGKKKFAD
                     ELSKLLLDYENLENSPDYVLIKPDGNSIKIAQIRNLQSDIVIRPHKDYKIYIINNAEK
                     MTVEAQNALLKTLEEPPNYAIIILVTNNKESLLETIKSRCDIIKFSPIPIEDLKRYLI
                     NTGIEEERAQLLAIFSRGSIENALNLSQSSEFSMMREDIQQYIQIMLDKNIVEILNIP
                     NNMEKYRGKIIALLDMMINYFRDIILLKENVNKNMLINVDKLVFIQNMSGKISYSQLS
                     KIIDIIEDAKSKIKSNCNFNISIQVMSLNIYEVIK"
     gene            3785..4678
                     /locus_tag="PC414_002308"
     CDS             3785..4678
                     /locus_tag="PC414_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HDF2464699.1"
                     /translation="MIKIVGIRFKSAGKIYYFDPVDFNIEQDMDVVVETARGLEYGKV
                     VVGPKDMDESELVSPLKPIIRIATEEDKKVYLENKEKAKETFELCQQKIKEHELTMFL
                     IDCEYTFDRNKLIFYFTAEGRIDFRELVKDLAAIFKTRIELRQIGVRDEAKSIGGLGP
                     CGRKLCCSSWLGDFQPVSIKMAKDQSLSLNPTKISGICGRLFCCLKYEHDVYSEAIEK
                     MPVVGALVQSDDGKGKVVEVNPLLEQIKVEFQDKTVKIYTREDIKILREPKKCDGCGG
                     KCKDNDGLDEATLRELKKLED"
     gene            4756..5502
                     /locus_tag="PC414_002309"
     CDS             4756..5502
                     /locus_tag="PC414_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HDF2464700.1"
                     /translation="MQLKDSERIDDLQLKGLKIIQDTNGFCFGIDAVLLANFTKIKKD
                     AKVVDLGTGTGIIPILIAGKSEAKKIIGVEIQEDVYEMATRSIKLNGLEERVEIINED
                     IKYIDKVLDINGYHVVTSNPPYMHIDGIKNPNDKKAISRHEVRCNLEDVIRAASRLVM
                     PRGKFFMIHRPTRLVDIITLGRKYKLEPKVIQFVHPRPQKAPNLVLVQFVKDGRPELK
                     ILDPLYVYGEDGNYTKELKAIYNNKDIGEL"
     gene            5503..6336
                     /gene="rsmI"
                     /locus_tag="PC414_002310"
     CDS             5503..6336
                     /gene="rsmI"
                     /locus_tag="PC414_002310"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HDF2464701.1"
                     /translation="MSGKLYICPTPIGNLEDITYRTLRTLKEVDLIAAEDTRHSIKLL
                     NHFEISKPLTSYHEHNKDSKGDYLINKLIDGENIALISDAGMPGISDPGEEIIKQAIQ
                     NNIEIEVLPGATAFVTALVGSGMDTHRFVFEGFLDRDKKVRKAQLEELKEESRTIIFY
                     ESPHRLKDTLKDMLKILGNRNISINRELTKKYQEIIRENIETTIKIFEEKEIRGEFVL
                     IVEGFHGEKSKKENYDGLTDREYVLKLIESGMNKKDAIKIVCKDRKLKKDVVYKQVLD
                     L"
     gene            6359..7063
                     /locus_tag="PC414_002311"
     CDS             6359..7063
                     /locus_tag="PC414_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HDF2464702.1"
                     /translation="MEIGKIIIESLKREDTHKIFIELDNKHELEKIIPKISCMKSVGE
                     CKYHVVNCFEHSINALKELEIVLNDKDFFPIHLRKHVSNYLNTYIEEGINKLHVLKLG
                     TFLHDIGKPDSMTLDETGRVHFTNHEKIGAQIIDNMGIKLELSTETYRLISKYVRYHM
                     ILLSLYKKNDLSRKELINVFNLVDEDTIGVIMLGYADIVSTVKLLGKSGEVSVLKTYM
                     EYILTNYLYKSNYTHV"
     gene            complement(7251..7679)
                     /locus_tag="PC414_002312"
     CDS             complement(7251..7679)
                     /locus_tag="PC414_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HDF2464703.1"
                     /translation="MDVTDYRIIEILQDDGRISMKDLGKIVGLTSPAVSERVKRLEES
                     GVIEGYKAIVNPDSLGRVIKAFIHISLPSNGYTEFIESAAKDPRIVECHHITGDDCLL
                     LKVIVKDMYELENVIDTIKKIGSTKTSVILSTPIQAKSIL"
     gene            complement(7716..9146)
                     /locus_tag="PC414_002313"
     CDS             complement(7716..9146)
                     /locus_tag="PC414_002313"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HDF2464704.1"
                     /translation="MRNLTLLTDLYQLTMLNGYFEKNIHEDIVVFDMFFRKNACDGGY
                     TIVCGIDQVVEYIDNLHFSDEDLEYLKNLNLFSDKFLKFLKEFKFTGDIYAVEEGTIM
                     FPNEPLITVKAPLYQAQLIETALLTIVNFQSLIATKASRVCFAAQGDPVFEFGLRRAQ
                     GPDAGIYGARAAVVGGCAGTANVLAGKMFDIPIIGTQAHSWVQKFDNELEAFQAYADV
                     YPDKCLLLVDTYDVLNSGVPNAIKVFKNISEKGYKPMGIRLDSGDLAYLSKEAKKQLD
                     NAGFSDISITASNDLDEYTITSLKAEGATINSWGVGTKLITSFDSPSLGGVYKLAASC
                     EKGVLEPKIKISENPEKINNPGYKKVIRIYNEDNKAEADLIMLHDEVIDESKPLEIFH
                     PTYTWKTKVFTNYKVKELLKPLYIKGRCKYNKKAVLEIKNHVQYELSTIWEQYKRLSK
                     PHIYKVDLSRNLWYLKTQMIDSKKVL"
     gene            9278..9862
                     /locus_tag="PC414_002314"
     CDS             9278..9862
                     /locus_tag="PC414_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein A"
                     /protein_id="HDF2464705.1"
                     /translation="MIIIGKIWFYMVSIGIIGSLISKNMGELNKVILSETSKGIEFAI
                     SLAGIMALWMGIMNIAKESGLIEKIGQKLNPIMRRLFPSIPKGHKAMSYIVMNIALNM
                     LGAGNGATAFGLKAMSELQTLNNKKDTATNDMIMFMVINISSIQIIPFTIIKLRMDMG
                     AQNPSEIIFTTLFATIISTAVAIITCKFFQRRYR"
     gene            9878..10399
                     /locus_tag="PC414_002315"
     CDS             9878..10399
                     /locus_tag="PC414_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein B"
                     /protein_id="HDF2464706.1"
                     /translation="MEIFSNLLIPIIILYIVIYGKYKKIDVYDSFVKGAIDGLKAAWD
                     ILPYIIGIFLAIGIFKTGKGLDMLEWLFTPIANMMSIPKELIGLISVKPLSGSGALGM
                     YSELANRVGIGSLVEKMGATIVGSSETIFYTMAIYYGSLKIKNTRHTLSCAMISHVAG
                     VIAAVFICYVIFV"
     misc_binding    10465..10711
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            10795..12732
                     /gene="metG"
                     /locus_tag="PC414_002316"
     CDS             10795..12732
                     /gene="metG"
                     /locus_tag="PC414_002316"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDF2464707.1"
                     /translation="MSKPSFYVTTPIYYPSGGLHIGHTYSTVAADTIARFKRFCGYDV
                     KFLTGTDEHGEKIQKKAIEQGMSEIEYLDGMIKDIKALWNTMDISYDDFIRTTEKRHT
                     DIIQKIFTKLYEQGDIYKGEYEGRYCTPCESFWTESQLLEGNKCPDCGRETYLVKEES
                     YFFRLSKYEDRLKELFKDDSFCFPAARKNEMVANFLDKGLEDLSVTRTTFDWGIKVPF
                     DEKHVIYVWVDALCNYITALGYMTDNDEEFKKYWPANVQIVGKEIVRFHTIIWPALLM
                     ALGLEVPKQVFGHGWILFADDKMSKSKGNVVYPEPIIERYGIDTLKYFLLREFAFGQD
                     GSYTHRNFVTRINYDLANDLGNLISRTVAMVEKYNNGIIPTAKVSTDFDADLKEQAVS
                     TRENFEAEMDKMQFHEALESVWKLVRRTNKYIDETMPWALAKDETKKDELDTVLYNLC
                     ESIRIIATLINPIMNETANKIYEHIGIKGQDDITTWESTKTFGLIGENVKVFKGEPLF
                     PRLDVEKEIEELTKMFSGKPPVEEKPLEHKEEITIDDLDKIELRVGKIISCEKHPKAN
                     KLLVSQVKIGPETRQIVSGIAEYYKPEDLVGKEVTVVCNLKPVKLRGVESQGMILAAG
                     DDGDPYVLPFTQGAKDGCEVR"
     gene            12762..13532
                     /locus_tag="PC414_002317"
     CDS             12762..13532
                     /locus_tag="PC414_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDF2464708.1"
                     /translation="MLFDSHAHLNDESFDEDRDELIGSLKDKGVDLVVNPGADIETSI
                     TAIELAKKYDFIYSAVGVHPHDVSKLDDTAIETLRKLATENEKVVAIGEIGLDYYYDY
                     SPREEQKEWFKKQIELANELKLPIIIHDRDAHGDTFEIIKNTKNPEIGCVLHCYSGNV
                     ELAREYVKMGCYISIPGTVTFKNNKKTREVVREIPLERLFIETDSPYMSPEPHRGKRN
                     NPSQVSFVADKIAQEKGISYEEVCRVTKENAKKFFNIK"
     gene            13870..14301
                     /gene="phnG"
                     /locus_tag="PC414_002318"
     CDS             13870..14301
                     /gene="phnG"
                     /locus_tag="PC414_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HDF2464709.1"
                     /translation="MYTVDSTKILINMNLEKLEKIYSMIKDTSSISIIKEPNLATVMV
                     RANESVKNTTFNLGEILVTECSVKVDESLGYGIVSENNNKKAIYLAVIDAVLHSNNSK
                     FDELKNYINKSVYEENLRYEQEIIDEFSLINKTKVQFNAMD"
     gene            14316..14891
                     /gene="phnH"
                     /locus_tag="PC414_002319"
     CDS             14316..14891
                     /gene="phnH"
                     /locus_tag="PC414_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HDF2464710.1"
                     /translation="MVNYTQEIYRKMLDSASFPGKLNNIDNINIENNTNLLNGAIGIA
                     ITLLDQEVTFYIENYTQKDIKNIRALTMSNQIDYRKSDYIFLDINSEVDILQIKIGSF
                     EYPDESATIVHQVNDLSTYSQTKYIKLDLSGPGIKSKNSIYINGIREEFIERLSSINK
                     DYPIGIDLILVDKKGEIAFIPRSSKLSWEVL"
     gene            14894..16009
                     /locus_tag="PC414_002320"
     CDS             14894..16009
                     /locus_tag="PC414_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-phosphorus lyase complex subunit PhnI"
                     /protein_id="HDF2464711.1"
                     /translation="MAYVAVKGGEEAILQAKNILEFYRVKGDSLPIDVKQIEEQMSYA
                     VDKVMSEGSLYNKELSALAIKQAQGDILEASFYVRALRSTMPRITYSEPINTEEMRIK
                     RRISATFKDIPGGQYLGATRDYENRILNVELLEEDEKKNRHFRDSFINELETVDEIPE
                     FTKISNIFRKENLLQEEVCENKLEEVIDDITRNSLRFPVSRAAKLQALTRGESGVVSG
                     FAYAIVRGFGDEHPYISELRTGNVFIKIKHPIYEDEEITIGDMLVTECEIVSKASQNK
                     TENSKNNSEDKNKMSLGYGLCMGNNENKAISMAILDKSLEPNSEKLAQDEEFVLLHID
                     GIDSLGFISHFKLPHYVDFKADVERIKKFEEVSLGDI"
     gene            15999..16868
                     /locus_tag="PC414_002321"
     CDS             15999..16868
                     /locus_tag="PC414_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HDF2464712.1"
                     /translation="MIYNYGFLDENTKKEIRRKILKAISIPGYQVPFGSRELPIAKGW
                     GTGGLQLTLSLIGKNDVVKVIDQGSDDSTNACNIRSFIASVSNVETTKDTLEATLIQT
                     RHRIPEEKLKSNQILIFQVPIPETLRIVEPSEVETRRMHSEEDYSRMWVYLYEDIVRF
                     NDISIAVEYPCKVNDRYLMNPSPIPRFDIKKLNMSDNLFLFGAGREKRIYAIPPYTKV
                     EPLEFEDYKFEEEKFEGKYCSLCNSTNTFLDEVYDSDTNEKYYSCSDTSYCEKVRLKN
                     NSIDVTIGGTWNE"
     gene            16861..17727
                     /locus_tag="PC414_002322"
     CDS             16861..17727
                     /locus_tag="PC414_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2464713.1"
                     /translation="MSKINDSKRLENIDKQVLIGRNINLIYGDGCEKCFESTGYESNN
                     ICKYCGSIVACNNVNLELYEGEVLGIVGESGSGKSTLLKILFFQEHANSGEVYISHYN
                     KDTNILELSDQKKRYIKNHFMGIVYQNPHLGLNLDFSSGGNIAEKLLMANLYNVEKIR
                     DRGKELLIKTNIPVERIDHKPKYFSGGMQQRVQIAKALSNNPPILLLDEVTTGLDVSV
                     QAEVLDLIREIQREFKISMIVVSHDFDVIKMLADRTIVMKNGSVIESGLTDQIMEDPQ
                     HPYTQQLINSLL"
     gene            17768..18481
                     /locus_tag="PC414_002323"
     CDS             17768..18481
                     /locus_tag="PC414_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2464714.1"
                     /translation="MKETILEVNNFSKDFELHALNKTITACSNISFEVSKGEFLGIIG
                     KSGAGKSTILKSIYKTYIPTTGEIIFNSEIYGNVDLAKIGDREMINLRKKEIGYVSQF
                     LKTLPRITAIELVVHSLIESGFEKDCSYDMAKDILNQFEIKENLWDAYPNNFSGGEKL
                     RLNLAQAMVKKPRLLLLDEPTASLDNQSKIYVKEKMLELKSQGTTMIGIFHDIEFMET
                     VIDKTFTMTKGTISESGVA"
     gene            18482..19651
                     /locus_tag="PC414_002324"
     CDS             18482..19651
                     /locus_tag="PC414_002324"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HDF2464715.1"
                     /translation="MIKLIHNANIVLEDMILENGHLIIEGDTIKKVSNDKIDYAFDFN
                     EIDEIIDAKNLYVIPGIIDIHSDAIEKEIEPRPSTLLPFNMAFYELDKKLPANGITTV
                     YHSISLGDGVGVRSIDNSLKMIKNIDSYKNIDSKSINHKVHLRYEVLYYEGLEKVLEL
                     LDENKIDYLSIMDHSPGQGQYTNPTFYKEYATKVWGVTENYVDTWLDDLVNLHDNLDW
                     NKIANIIGIAKTKNINVASHDDDTLEKMDFIKNIGINVSEFPITLEVAKHSKKLGVLT
                     CLGAPNIVRGKSHNNNLKAMDAILEDCCDIVCSDYLPSAMIKSMCIVAERINNLNKAV
                     SLFTSNPAKAVGIYDERGSIKENKKADLVLVDIDSEYPKVVNTIVNGKTVYKREV"
     gene            19655..20491
                     /locus_tag="PC414_002325"
     CDS             19655..20491
                     /locus_tag="PC414_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HDF2464716.1"
                     /translation="MLTELHCHTSISDNNFTTEEIIKKAAEKNINILAITNHDTLSGL
                     DEALVLGEHYGITIIPGIEISAYDYKNRKRVHILGYNIDLNSQEIKNLCNPMVCDRHK
                     ASVKMVNKIIDLGYKISLEDVKKYSSKTGIFKQHIMKALIDKGYTDNIYSSLYKELFH
                     RGNGKVYVSLKYVDYRDAIKAIKNSGGICVLAHPGQMDNFSAIEEMVKVGLDGIEVYH
                     PSHNKELEKESLSYAKKYNLVVTGGSDYHGFYSQHNYELGSKSLNKEDLFKFRLALGG
                     VL"
     gene            20492..21487
                     /locus_tag="PC414_002326"
     CDS             20492..21487
                     /locus_tag="PC414_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2464717.1"
                     /translation="MSFLQVKNVGKSYGQVKVLKDISIDIEKGEFICLLGPSGCGKST
                     LLRIIAGLEDKHGGKIIINDKDMTNSPPESRNFGIVFQSYALFPNMNVYKNIAFGLEN
                     KNISKSNIDKKVKEVLEVVELSGYEKKYPSQLSGGQQQRVALARAIALEPDFLLLDEP
                     LSALDAKVRLKLREQIRSLHRKLGITTIMVTHDQEEALCLADKMVVMNRGEIIQVGTP
                     KEVYKNPETPFVADFIGTINFIDDGINKIAIRPEDIKVESNRDSKDKDIKVGEILDIE
                     FRGFNYRITVEYRSKQMKLDVVSKVAEQMKLCIGSKINFKIPKEGIVQYKSEGCA"
     gene            21505..23193
                     /locus_tag="PC414_002327"
     CDS             21505..23193
                     /locus_tag="PC414_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HDF2464718.1"
                     /translation="MITSKNKRRISDSTIQKYFLIFSVIVLIVFILFPLVSLLKNAFM
                     DTSGNFIGISNFSKYVENPSLIASFKNSIFVSTVSSLISLCLAFIYSYAITRTNIKFK
                     EFFKTMGMFPLFAPTMLYGISLIYLFGNKGIFTTMGLEVPLYGPLGIIISQILFTFPQ
                     AFLILSVALSMADYRLYEAADSLGASEFKKFCKITIPGIKYSLLSSFFVSFILAFTDF
                     GAAKVVGGNYNVLATDIYKQVVGQFNIPMGATVSMVMLIPVLIAFTLDKMASKKQGMT
                     ITAKSMPYKIKENKFRDRVFLIVCSFITLLIFILIFVSVIGSVIKLWPYNLSFTMEHY
                     TFKGILGSGIKTYLSSLKISTLTAIFGTIFVFFSAYMIEKVDKFPKLRQTAYLLSMIP
                     MALPGLVLGISYITFFNSASNPLNFLYGSTAILVIVNVVHFYSVCFITSNSSLKMLDK
                     EYELVAKSINIPFYKVFFNITVPMSITSILEIVVYYFVNSMVTVSALIFLYTPQTQTA
                     SISILKLDEIGYIGPSAAMAVLVLLTNIIVRLLYEFVTKKLKNNTQKWQQKDVA"
     gene            23205..24272
                     /locus_tag="PC414_002328"
     CDS             23205..24272
                     /locus_tag="PC414_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2464719.1"
                     /translation="MKFKKLVSLGVLSTLLLSVITGCSTDSSGSDSKSDSKTKGDLTV
                     YTAIEEDSIEPYLATFKEKYPDIKLNIVRASTGDITARLLAEKDNPQADVVWGVAATS
                     LLVADDQGMLEPYAPKGSEEIESAFKDDKEVPSWVGIDAWMTGITVNTKELSDKNIPV
                     PSSYEDSIKPEYKGLISMPNPSSSGTGYLTVSALIQIMGEEKAWQYMDKLHENIGVYT
                     QSGSAPATSAASGEYPIGISFGYRGIKLKEEGYPVEVVFPKEGSGWDIEANALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNYAVTTISTGNPIPEGFPKKPLEQMIDNDLKSAAK
                     NREDILNKWISKYDGKTEKES"
BASE COUNT         9767 a         2751 c         4377 g         7598 t
ORIGIN      
        1 tattaaaaac ttaatagaaa tattaagcat aaagattatg tggttacaat agcagagagg
       61 atacacctgt tcccattccg aacacagaag ttaagctctc tagcgctgat ggtacttggt
      121 gggaaactgc ctgggagagt aggacgtagc cacgtaattt tttttatttt ttgtcaaaaa
      181 aaacaaaaaa tacgtatttc ttttttcatt tcgggaaaac taaacacagg aattatggac
      241 tttataaaaa tttaattctt gaggagtgat ataagtgaaa aaagttgctt taattttagc
      301 tataataggt gcgcttaact ggggtgcaat tggtattcta ggtacagact taataggtag
      361 tatctttggt ggaacatatg aaatggtaag tagaataata tacttcattg taggtttagc
      421 tggattatac ttaataccaa gtttaatgtc tgatgataga gaataattta tgcttttatg
      481 ttaaatacct cattctaaat gtttagaatg aggtatttaa ttttataaaa atattaattt
      541 gtttcttttt taatggtaaa atagataaga attgaagttt taaaaagaag tattatgtaa
      601 aaaaggagta ctattaatat atgtttatta ttaatgaatt aaataagatt gtaaatgaag
      661 aagtaatatc ttttcatatg cctggtcata aaaagggaca ggtttataaa aagttaggat
      721 ataaagatat actaaaaaat ttgtataaaa tggatacaac tgagatatta ggaacagata
      781 atttacactt tccggaagga attataaaaa attcacaaaa aaaagcatca agagtattta
      841 aaagtgatta tacttgctat ttagtaaatg gtagtagctg tggaatacaa tctgcaataa
      901 tgtcagtatg tagtcctaaa agtaaaataa taacaaatag agactgtcat cagtctgtta
      961 taaatggatg tattttagga gatatagata tagagtatgt gccttgtgag ataagtgaag
     1021 agacaaatgt cttaaaaggg atatctataa gtaatgtgat tgatataata gataaaaact
     1081 tggatgcaaa agcagtactg cttacttatc ctacatatta tggaatgaca tatgatttag
     1141 aatacatatg taattatgca catagcaaaa agatggtagt gatagtagat gaagcacatg
     1201 gtgcgcattt agggcttagc gagaggcttc ctaaaacagc tcttgagcaa ggcgcagata
     1261 tagttgtaca gagtacacat aaaactcttc cagcatttac acaatcgtca atgatacata
     1321 tacagggaga ccgagttgat atagataaaa tattgaacat gttaagaatt acagaatctt
     1381 caagcccttc atatttatta ttatcttctc tggaattagc agtagatata tatgaaaata
     1441 aaggaaaaga attaatggaa gagcttttaa ataatattat aactttcaaa aataaagttg
     1501 attcaaatat taatatatac gacacaaatg ataaaacaaa aatatttata tcatctaaaa
     1561 atatgggttt aacaggttat gaactagaaa atattttaag aaacaaatat aacatacaag
     1621 tggagttatc taattattat ggagttcttt taatatgtac aataggaaat actgttgagg
     1681 atttctctag tttagaaaca gcattaaaca atattgtaaa ggaagacttt aaatttgaaa
     1741 agttagaaaa taaaaaatac cctgttgtta ttccaaagaa aatattaaca cctagagaag
     1801 cattttataa aactaaaaaa agtgttaaaa tatatgacag cataggtaaa gtttgtggtg
     1861 aatgtatagt tccataccca cctggaataa gtgtaatatc tcctggtgaa gtaataagta
     1921 aagaaataat agactatcta atgttttgtc attctaaagg catgattgtg agtggattga
     1981 aagatgtaga tttaaatttt atacaaataa tagatttaga agacatttaa aaattatgat
     2041 ataaaagtag atagaagtag atagtttaaa attgtgaaga gaaataaata gactgcttat
     2101 atagagctat ctaataaaag tactaaaaaa gtaaaaagtg tttgtatgga ggatatgaaa
     2161 atgaaaggta agttaataat tatagagagt ggttcagatg caagtggaaa ggctactcag
     2221 acagaaatat tatatgaaag attaaaaaaa gaaggctata tgattaaaaa ggtagaatat
     2281 ccaaattatg aatctccatc atctgccttg gtgaaaatgt acctaaaagg agactttggt
     2341 aaaaatgcat cagatgtaga cccatatata gcatcaactt tttttacagc tgataggtat
     2401 gcatcattta aaaccgattg ggaggagttt tacaatgaag gtggaataat tatcgcagat
     2461 agatatacaa cctctaatat ggtacatcag gcctctaaaa tggatatttg tgatagagaa
     2521 gagtatttaa attggttagc tgattatgaa tttaatttat ttaagatacc acaacctgat
     2581 tgtgttatat ttttagatgt gcctattgaa tttagtaaaa agcttatgga gaatagaaaa
     2641 aataaaatta caggggaaga aaaaaaggat attcatgaaa gtgacataga gtatttagag
     2701 aaatcttaca acaatgcttt atatatagca gataaatatg attggaaaaa aataaattgt
     2761 gttttagata acaaattaag aagtattgaa agtatacata atgaaattta taaaatagtt
     2821 cttgattcaa taaaacctat ggagagataa atatgtattt tgaaaatatt atagggcaag
     2881 attttgcaaa gaaatattta acaaattcta taaagaaaaa taaacttaat aatgcgtata
     2941 tgtttgaagg aatggatgga ataggaaaaa agaaatttgc agatgaatta tcaaaactat
     3001 tacttgatta tgaaaatctc gaaaatagtc cagactatgt actcataaaa ccagatggaa
     3061 atagtataaa aatagctcaa atcagaaatt tacaatcaga tatagtaata agacctcata
     3121 aagactataa aatatatata atcaataatg ctgaaaaaat gactgtggaa gctcaaaatg
     3181 cacttttaaa gacgctagag gaacctccaa actatgctat aataatctta gtcacaaaca
     3241 acaaggaatc tttattagaa actataaaat ctagatgtga tataataaag ttctcaccta
     3301 taccaattga agacttaaaa cgttatttga taaatacagg tatagaagaa gaaagagctc
     3361 aattattggc tattttttcg agaggaagta ttgagaatgc tctaaatcta tctcagtctt
     3421 ctgagttttc aatgatgaga gaagatatac aacagtatat tcagataatg ttagataaaa
     3481 acatagtgga gattttaaat ataccaaata atatggaaaa atatagaggt aagattatag
     3541 ctttattaga tatgatgata aactattttc gagatatcat cctattaaaa gaaaatgtaa
     3601 acaagaacat gttaataaat gtagataagc tagttttcat tcaaaatatg agtggaaaaa
     3661 ttagttattc tcaattgtcc aagattattg atataataga agatgcaaag agtaaaataa
     3721 aaagcaattg caatttcaat ataagcatac aagttatgtc tttaaatata tacgaggtga
     3781 ttaaatgata aagatagtag gtataaggtt taaaagtgct gggaaaatat attattttga
     3841 ccctgttgat tttaatatag agcaagatat ggatgtagtt gtagaaactg caagaggctt
     3901 ggagtatggg aaagtagtcg ttggtccaaa agatatggat gaaagcgaat tagtttctcc
     3961 tttgaagcca attataagaa tagctacaga agaagataaa aaagtatatc tagaaaataa
     4021 ggaaaaggca aaagaaactt ttgagttgtg ccaacaaaaa ataaaagaac atgagttaac
     4081 aatgttttta atagattgtg agtatacatt tgataggaat aagttgattt tttactttac
     4141 agcagaagga agaatagatt ttagagaatt ggtaaaagac ttagctgcta tatttaaaac
     4201 tagaatagaa cttagacaaa taggtgtcag agatgaagct aaatctatag gaggtctagg
     4261 gccttgtggt agaaaacttt gttgttcatc ttggttaggt gattttcaac cagtttcaat
     4321 aaaaatggca aaagaccaaa gtctatctct aaatccaaca aaaatatctg gaatatgtgg
     4381 gagattattc tgttgcttaa aatatgagca tgatgtatat agtgaagcaa tagaaaaaat
     4441 gcctgttgta ggtgcattgg tgcaatcaga tgatggaaaa ggaaaagttg tagaagtgaa
     4501 tcctttatta gaacaaatta aagtggaatt tcaagacaag acagtaaaga tatacacaag
     4561 agaagatata aaaatactta gagagcctaa aaaatgtgat ggctgtggtg gtaaatgtaa
     4621 ggataatgat ggattagatg aagccacact aagagagcta aagaaattag aagattaaat
     4681 ttaaggtagg gcttttccta ccttttttaa agcaattaat gcttaaagtt attaatattt
     4741 gaggagaatt tatatatgca attaaaagat tcagaaagaa tagatgattt acaattaaaa
     4801 ggccttaaaa ttatacaaga tactaatggt ttttgttttg gtatagatgc agttttatta
     4861 gccaatttta ctaaaataaa aaaagatgcc aaagtagtag atttgggtac tggaactgga
     4921 attataccta ttttgatagc tggaaaaagt gaagctaaaa aaattattgg cgtggaaata
     4981 caagaagatg tatatgaaat ggcaactcgt tctataaaat taaatggatt agaagaaaga
     5041 gttgagatta taaatgaaga tataaaatac atagacaaag tcttagacat aaatggttac
     5101 catgtagtca catcaaatcc tccatatatg catattgatg gaataaaaaa tccaaatgat
     5161 aaaaaagcaa tatcaagaca tgaagtaagg tgcaatttgg aagatgtaat tagagctgca
     5221 tccagattgg ttatgcctag aggaaaattc ttcatgatac atagaccaac aagattggtt
     5281 gatataataa ctttagggag aaaatataaa ttagaaccaa aggttattca atttgtgcat
     5341 ccaagacctc aaaaagcacc gaacttagtt ttggttcaat ttgtaaaaga tggaaggcca
     5401 gagcttaaaa tattggaccc attgtatgtt tatggagaag atgggaatta tacaaaagaa
     5461 ttaaaagcaa tatataataa taaagatata ggagagttgt aaatgagtgg gaaattgtac
     5521 atatgtccaa caccaatagg aaacctagaa gatataacat atagaacttt aagaacatta
     5581 aaagaagtag acttgattgc agcagaagat acaagacata gtataaaact tttaaatcat
     5641 tttgaaatct caaaaccact aactagttat catgaacata ataaggattc aaagggagat
     5701 tatttgataa ataaactaat tgatggtgaa aatatagctc tgataagtga tgcaggtatg
     5761 cctggtatat ctgacccagg agaagagata ataaaacaag ctatacaaaa taacatagag
     5821 atagaagtat tgccaggtgc aacagccttt gtaacggctt tagttggctc tggtatggac
     5881 acacatagat ttgtttttga gggcttttta gatagagata aaaaagttag gaaagcacaa
     5941 ttggaagaat taaaggaaga aagtagaact ataattttct atgaatcacc acatagattg
     6001 aaggatacac taaaagatat gcttaaaatt cttggtaata ggaatatttc tataaataga
     6061 gaacttacaa aaaaatatca agaaataata agagaaaata tagaaactac cataaaaata
     6121 tttgaggaaa aagaaataag aggagaattt gtattaatag tagagggttt tcatggtgag
     6181 aagtcaaaga aagaaaatta tgatgggctt acagataggg aatatgtttt aaaactaatt
     6241 gaaagtggta tgaacaaaaa agatgcaata aaaatagttt gtaaagatag aaagttaaag
     6301 aaagatgtgg tttataaaca agttttagat ttataaaacc tgaatctgag gtgattttat
     6361 ggaaataggg aaaattataa tagagagttt aaagagggaa gatacacata aaatatttat
     6421 agaattagat aataagcatg aattagaaaa aataattcct aaaataagct gtatgaagtc
     6481 tgtaggtgaa tgtaaatatc atgtagtaaa ttgttttgaa cattctataa acgcattaaa
     6541 agaattagaa atagtattaa atgataagga tttttttcca atacatctaa gaaaacatgt
     6601 gtcaaactat ttaaacacat atattgaaga aggaataaat aagttacatg tattaaagct
     6661 gggaacattt ttacatgata taggtaagcc tgacagtatg accttagatg aaaccggaag
     6721 agttcatttc acaaatcatg aaaaaatagg tgctcaaata attgacaata tgggaataaa
     6781 gttagagtta tcaacagaga catatagact tataagtaaa tatgtaagat atcatatgat
     6841 acttttgtct ctatataaga aaaatgattt gagtaggaaa gaattaatta atgtatttaa
     6901 cttagtagat gaggacacta taggtgtaat aatgcttggg tatgcagata tagtgtctac
     6961 agtaaaatta ttgggcaaga gtggagaagt cagtgtgtta aagacatata tggaatatat
     7021 tttaacaaat tacttgtata agagtaatta tacacatgta taaatttagt taatataatt
     7081 gagttttaga tgattaattg aagtttgaaa tatacggatt tcaaaaacat aaaagaagtt
     7141 gtctttgttg taaaattatg aaagacaact ttttttatat caaaaattga aattttatta
     7201 aggatactta atggtctatt aattaataaa ccattagtat ttaatttata ttacaatatt
     7261 gattttgctt gtattggcgt tgatagtata acagaggtct tagtagaacc aattttcttt
     7321 atagtatcaa tcacattttc taactcgtac atatctttta caattacttt taaaagtaag
     7381 caatcatcac ctgttatatg gtgacattct acaatcctag ggtcctttgc agctgactca
     7441 ataaattctg tatatccatt gcttggaaga gaaatatgaa taaatgcctt tataactctg
     7501 cctaatgaat ctgggttgac aatagcttta tatccttcta taacaccaga ctcttctaat
     7561 cttttgactc tttctgaaac tgcaggagaa gttaaaccaa ctatttttcc taagtccttc
     7621 atagaaattc tcccatcatc ctgtaaaatt tctatgattc tgtaatctgt aacatccata
     7681 aataaacagt cccccttaaa taaaaataat taatgctata aaactttttt agaatctatc
     7741 atttgtgttt ttaaatacca aagatttcta gataaatcaa ccttataaat gtgtggtttt
     7801 gaaagtcttt tgtattgttc ccagatagta cttaattcat attgtacatg attttttatt
     7861 tcaagaacag cttttttatt atatttgcat ctgcctttta tatacaatgg tttaagaagc
     7921 tctttaactt tgtaatttgt aaataccttc gttttccatg tataagtagg atggaagatt
     7981 tctaaaggtt tagactcatc tataacttca tcatgtaaca taatcaaatc tgcctctgcc
     8041 ttattgtctt cgttatatat tcttataact ttcttgtaac caggattatt tattttttct
     8101 ggattctcag aaatctttat cttaggttca agaactcctt tttcacaaga tgcagctaat
     8161 ttataaactc ctccaagaga aggtgaatcg aaagatgtaa tgagctttgt acctactccc
     8221 catgaattta tagtagctcc ttctgcttta agtgatgtta tggtatattc gtctaggtca
     8281 ttagatgcag tgatgctgat atctgaaaag ccagcattat ctaattgttt tttagcttct
     8341 ttagataaat aagctaaatc tccagagtca agtcttatac ccataggttt gtatcctttt
     8401 tcagaaatat ttttgaatac ttttattgca ttaggaactc cactgtttaa tacatcataa
     8461 gtatctacaa gtagtaggca tttatctgga taaacatcag catatgcttg gaatgcttca
     8521 agttcattat caaatttttg tacccaactg tgagcttgtg taccaattat aggtatatca
     8581 aacattttcc ctgctagtac gtttgcagtt ccagcacaac caccaactac agcagcccta
     8641 gccccatata tccctgcatc tggaccttgt gcacgacgta atccaaattc aaatacaggg
     8701 tctccttgag ctgcaaagca aactctagag gctttagtag caataagaga ctgaaaatta
     8761 actattgtta acaatgctgt ttcaataagt tgagcttgat ataaaggtgc cttaacagta
     8821 attaatggct cattagggaa catgatagtt ccttcctcaa cagcatatat atctcctgtg
     8881 aatttaaatt cttttaaaaa ttttaagaac ttgtcagaaa ataaatttaa gttttttaag
     8941 tactctaaat cttcatctga aaaatgaaga ttatctatgt attcaacaac ctgatcaata
     9001 ccacaaacta tagtataccc tccatcacaa gcgttttttc taaaaaacat atcaaataca
     9061 acaatgtctt catgaatatt cttttcaaaa tagccattta acatagtaag ctgatataaa
     9121 tcagtaagaa gtgttagatt tctcattatt tctctcctta attattatta aatattaaaa
     9181 attgtaaaaa ctcccacact tatactaaaa cataatatag tacaactttc aagtataaag
     9241 tattacctaa ctatcataaa attatttaaa gaggaggatg attataatag gaaaaatatg
     9301 gttttatatg gtatcgatag gaataatagg aagcttgatt tctaaaaata tgggtgaatt
     9361 aaataaagtt atattaagtg aaacatcaaa aggtatagag tttgcaataa gtctagctgg
     9421 tatcatggca ctctggatgg ggataatgaa tatcgcaaaa gaatcaggtt taattgaaaa
     9481 gataggtcaa aaactaaatc ctataatgag aagattattt ccttctattc caaaaggtca
     9541 taaagcaatg tcttatatag ttatgaatat tgctttaaat atgttgggtg ctggaaatgg
     9601 tgcaacagca tttggtttaa aagcaatgag tgagcttcaa actttgaata acaagaaaga
     9661 tacagccact aatgatatga ttatgtttat ggtaatcaat atatcatcta tacaaataat
     9721 tccatttacc ataatcaaac taaggatgga tatgggggct caaaatccaa gtgaaataat
     9781 ttttacgact ttatttgcaa ccataatatc tacagcagta gcaataataa catgtaaatt
     9841 ttttcaaaga agatatagat aaaggggatg tagatacatg gagatatttt caaacttact
     9901 tataccaata ataattctgt acatagtaat ttatggtaaa tataaaaaaa tagatgtgta
     9961 tgatagtttt gtaaaaggag caattgatgg attaaaagct gcatgggata tcctaccata
    10021 tataataggt atatttttgg caataggaat attcaaaact ggtaaaggtc ttgatatgtt
    10081 agaatggcta tttacaccaa tagcaaatat gatgagtata ccaaaagaat taataggtct
    10141 aataagtgta aaacctttat ctggaagtgg tgctttaggg atgtacagtg aacttgcaaa
    10201 tagagttgga ataggaagtt tagtagaaaa aatgggtgct acgattgtag gttcttctga
    10261 gactattttt tacacaatgg caatatacta tggaagtctg aagataaaaa atactagaca
    10321 tacattatca tgtgctatga ttagccatgt tgcaggtgtc atagcagctg tttttatttg
    10381 ctatgtaata tttgtataat ttgttgacaa tatgggaaaa attttataat atagtgatta
    10441 tatagaaaca aatagtaata caaattacaa gctatgaaaa gaagagtaaa taaaggtttt
    10501 ttctatcaga gagctaggtt aggtgaaagc tagtgtcaaa agctttattg aagatggtct
    10561 tggagctttt tatctgagca agtgtgctag ggtaaaacgg ttggacacgt tatagtctac
    10621 taaggtatct ataaaatgtt tctacatttc atgtgaaaca taactattat agatgaatta
    10681 gggtggtacc gtgaataatc tcgcccctat gttaactcat aggaggcgat tttttattat
    10741 ttaaatgaaa gaatcatatg attacgaacg ataaaaaatt aggaggtaaa taacatgagc
    10801 aaaccgagtt tttatgtaac aacaccaata tactatccaa gtggaggatt acacatagga
    10861 catacttatt caacagtagc tgcagataca atagctagat tcaagcgttt ctgtggatat
    10921 gatgtaaagt tcttaacagg aacagatgag catggtgaaa aaattcaaaa gaaagccata
    10981 gaacaaggaa tgtcagagat agagtatctt gatggaatga taaaagatat aaaggcctta
    11041 tggaatacta tggatatatc ttatgatgat tttataagaa ctacagaaaa aagacataca
    11101 gacataatac aaaagatatt tactaaacta tatgagcaag gtgatatata taaaggtgaa
    11161 tatgaaggta gatactgtac tccttgtgag agtttctgga cagaatctca attactagaa
    11221 ggaaataaat gccctgactg tggaagagaa acatatttag taaaagaaga atcttatttc
    11281 tttagattat ctaaatatga agacagatta aaagagttat ttaaagatga tagtttctgt
    11341 tttcctgctg ctagaaaaaa tgaaatggtg gcaaactttt tagacaaagg attagaagat
    11401 ttaagcgtaa ctagaacgac ttttgactgg ggtataaaag ttccttttga tgaaaaacat
    11461 gtaatttatg tgtgggtaga tgctttatgt aactatataa cagcattagg ttatatgact
    11521 gataatgatg aagaatttaa aaaatattgg cctgccaatg ttcaaatagt aggtaaagaa
    11581 atagtgagat tccatacaat aatatggcca gcgcttttaa tggctttagg tctagaagtt
    11641 ccaaaacaag tatttggaca tggatggata ctatttgctg atgataaaat gagtaaatca
    11701 aagggtaatg tggtttatcc agaacctata atagaaagat atggaataga cacacttaag
    11761 tacttcttat taagagagtt tgcatttggg caagatggaa gttatactca tagaaatttt
    11821 gtaacgagaa taaattatga tttagcaaat gatttaggaa acttaataag tagaactgta
    11881 gctatggttg aaaaatacaa taatggtata ataccaacag caaaagtttc aactgatttt
    11941 gatgctgact taaaagaaca agctgtttca actagagaaa actttgaagc tgaaatggat
    12001 aaaatgcaat tccatgaagc attagagagt gtatggaaat tagttagaag aactaacaaa
    12061 tatatagatg aaactatgcc ttgggcacta gctaaagatg aaacgaagaa agatgaatta
    12121 gacacagttt tatataattt atgtgaatct ataagaataa tcgcaacact aataaatcct
    12181 ataatgaatg agactgctaa taaaatatat gaacatatag gaataaaagg tcaagatgat
    12241 ataactacat gggaaagtac aaaaacattt ggtcttattg gagaaaatgt aaaagtattt
    12301 aaaggtgaac ctttattccc aagattagat gtagaaaaag agatagaaga attaactaaa
    12361 atgttctctg ggaaacctcc agtagaagaa aaacctttag agcataaaga agaaattact
    12421 attgatgatt tagacaaaat agagcttaga gtagggaaaa taataagctg tgaaaaacat
    12481 ccaaaagcaa ataaattatt agtatcacaa gttaagattg gaccagaaac aagacaaata
    12541 gtatcaggaa tagctgaata ctataagcca gaggatttag taggaaaaga ggttacagtg
    12601 gtatgtaact taaaaccagt taaattaaga ggtgtagaat ctcaaggtat gatattagca
    12661 gcaggtgatg atggagaccc atatgtttta ccatttacac aaggggctaa agatggatgc
    12721 gaggttcgtt agtaggtaat taaatagtaa ggagattaaa gatgttattt gactcacatg
    12781 cacatttaaa tgatgaaagt tttgatgaag atagagatga acttattggt tcattaaaag
    12841 ataaaggtgt agatttggtt gtaaatccag gagcagatat agagacgtcg ataacagcaa
    12901 ttgaacttgc gaaaaaatac gactttatat attcagcagt tggggtacat ccacatgatg
    12961 tatcaaaact agatgataca gcaatagaaa ctctaagaaa acttgcaact gagaatgaaa
    13021 aagttgtagc tattggagag attggattag attattatta tgattattct ccaagagaag
    13081 aacaaaaaga gtggtttaag aagcaaattg aattagctaa tgagctaaaa cttccaataa
    13141 taatacatga tagagatgca catggagata cttttgagat aataaagaat actaaaaatc
    13201 ctgaaatagg atgtgtactt cattgttata gtgggaatgt agagttagct agagaatatg
    13261 ttaagatggg ttgttatata tcaattccag gcacggttac ttttaagaat aataaaaaaa
    13321 ctagagaagt agtaagagaa atacctctag aaagattatt catagagact gattcaccat
    13381 atatgtcacc agagccacat agaggaaaga gaaacaatcc ttcccaagta tcttttgttg
    13441 ctgataagat agcccaggaa aaagggatat cttatgaaga agtatgtaga gttacaaaag
    13501 aaaatgcaaa gaaatttttt aatataaaat agacaaatta aatcttataa tgatatatat
    13561 aataagtcat tataagtata ggatgagagt gcctcaaaat gagtttttta gttcatgagg
    13621 cactcttttt tgtatgaaat caaatatatt ttcatcattt caacaatgaa aaagaagttt
    13681 atcatatttt gagatagttt gtttttattt atttaattca tattttaaaa tatattttct
    13741 taacattgtt aaaaaagaat ttacaagaga ttcattagaa ttaactcaac tctaatattc
    13801 acttaactta agttgtttat tatattatta aagagaagta aacgaaaggt agattaagga
    13861 ggttgaattt tgtatacagt tgatagtact aagatattga taaatatgaa cttagagaaa
    13921 ctagaaaaga tatactcaat gataaaagat acatcaagta tttctataat taaagaacct
    13981 aacttggcta cagtaatggt aagagctaat gaaagtgtaa aaaacacaac atttaattta
    14041 ggagagatat tagttactga atgtagtgta aaggtagatg aaagcttggg ttacggaata
    14101 gtttcagaaa acaataataa gaaagccatt tatttagcag ttatagatgc agttttacat
    14161 agcaataaca gtaaatttga tgagttaaag aattacataa ataaaagtgt atatgaagag
    14221 aacttaaggt atgaacaaga gattattgat gaatttagct tgattaataa aacgaaagtt
    14281 caattcaatg ctatggatta aataggagga gaatcatggt aaattataca caagaaatat
    14341 atagaaagat gctagatagt gcttcatttc ctgggaaatt aaataatata gataatataa
    14401 atatagaaaa taatacaaat ctattgaatg gagcaattgg tatagcaatc actttattag
    14461 accaagaagt taccttctat atagaaaatt atactcaaaa agatataaaa aatataagag
    14521 cacttacaat gtcaaatcaa atagattata gaaaatcaga ttatatattt ttagatatta
    14581 attcagaagt ggatatatta cagattaaaa taggttcttt tgaatatcct gatgaaagtg
    14641 caactatagt acatcaagta aatgatttat caacgtactc tcaaactaag tatataaagc
    14701 ttgatttgag tggtcctggt ataaaatcta aaaatagtat atatataaat ggaattagag
    14761 aagaatttat agaaagatta tctagtataa ataaagatta tccaatagga atagatttga
    14821 ttttagtaga taaaaaagga gaaattgcat ttataccaag gtcatcaaaa ttaagttggg
    14881 aggtattata gaaatggctt atgtagcagt taaaggtgga gaagaagcca tattacaggc
    14941 taaaaatata cttgagtttt atagggtaaa aggagatagc ttgcctatag atgtaaagca
    15001 gattgaagaa caaatgtctt atgctgtaga caaagtaatg tctgaaggaa gtttatacaa
    15061 caaggaacta tcagcattgg ctataaaaca agctcaaggt gatatattag aagcctcttt
    15121 ctatgtaaga gctttaaggt caacaatgcc tagaattaca tattcagaac ccataaatac
    15181 agaagaaatg agaataaaaa gaagaatatc agctactttt aaagatatac caggaggtca
    15241 gtatcttggg gcaactagag actatgaaaa tagaatattg aatgtagaat tattagaaga
    15301 agatgaaaaa aagaatagac attttagaga tagttttata aatgaattag aaacagtaga
    15361 tgaaatccct gaatttacaa aaatatctaa tatatttaga aaagaaaatt tattacaaga
    15421 agaagtttgt gaaaacaaat tggaagaagt tattgatgat attacaagaa atagtttaag
    15481 atttccagtt tctagggcag caaagttaca ggcattaact agaggtgaaa gcggagtagt
    15541 atcaggtttt gcttatgcta ttgtaagagg ctttggtgat gaacatccat atataagtga
    15601 acttagaact ggcaatgtgt ttataaaaat aaaacatccc atctatgaag atgaagaaat
    15661 aacaataggt gatatgttgg ttactgaatg tgaaatagtt tcaaaagcat cgcaaaataa
    15721 gactgaaaat tctaaaaata attcggaaga taaaaataaa atgtctttag gatatggtct
    15781 ttgtatgggg aacaatgaga ataaggctat atctatggct atactagaca aatctttgga
    15841 gccaaatagt gaaaaactag cacaagacga agaatttgta ttgcttcata tagatggaat
    15901 agattcatta ggatttatat ctcactttaa gcttcctcac tatgtagatt ttaaagcgga
    15961 tgtagaaaga ataaaaaagt ttgaggaggt aagtttaggt gatatataac tatggttttt
    16021 tagacgaaaa cacaaaaaaa gaaataagaa gaaagatatt aaaagcaata tcaatacctg
    16081 gttatcaagt tccctttggt tcaagagagt tgcctatagc taagggatgg ggtactggag
    16141 gtttacaatt aacactatca ttaattggta aaaatgatgt agtaaaagta attgaccaag
    16201 gaagtgatga ttctacaaat gcttgtaata taaggagctt tattgcaagt gtaagtaatg
    16261 tagaaaccac aaaagataca ttagaagcta cattgattca aacgagacat agaataccag
    16321 aagaaaaact taaatctaat caaatactaa tatttcaagt accaatacca gaaaccctta
    16381 gaatagtaga acctagtgaa gtagaaacaa gaagaatgca ttcagaagaa gattatagta
    16441 ggatgtgggt atatctttat gaagatatag ttagatttaa tgatatatct atagcagttg
    16501 aatatccatg taaagtaaat gatagatatt taatgaatcc atcacctata cctagatttg
    16561 atataaaaaa attaaatatg tcagacaatt tattcttgtt tggtgcaggt agagaaaaaa
    16621 gaatttatgc tattcctcca tatacaaagg tagaaccttt agagtttgag gattataagt
    16681 ttgaagagga aaagtttgaa ggaaaatact gtagtttatg taacagtaca aatacatttt
    16741 tagatgaagt atatgacagt gacactaatg aaaagtacta tagttgctca gatacaagtt
    16801 attgtgaaaa agttagatta aaaaataaca gtatagatgt gacaatagga ggtacttgga
    16861 atgagtaaaa taaatgactc taaaagatta gaaaatatag acaagcaagt attaatagga
    16921 agaaatatca atcttattta tggagatggg tgtgaaaagt gttttgaaag cacaggatat
    16981 gaaagcaata atatatgtaa gtattgtggt tctatagttg catgtaataa tgtaaattta
    17041 gaactttatg aaggagaagt ccttggaatt gtaggtgaat ctggttcagg aaaaagcact
    17101 ttattaaaaa tactgttttt ccaagaacat gcaaatagtg gagaagtata tatatcccat
    17161 tacaataaag acacaaatat actagagctt tcagaccaaa aaaagagata cataaagaat
    17221 cattttatgg gtattgtgta tcaaaatccc caccttggtt taaatttaga ctttagtagt
    17281 ggtggtaata tagcagaaaa attattgatg gcaaatttat ataatgttga aaaaataaga
    17341 gatagaggca aagaactttt aataaaaact aatatacctg tagaaagaat agaccataaa
    17401 ccaaaatact ttagtggggg aatgcaacaa cgagttcaaa tagcaaaagc tctttcaaat
    17461 aaccctccaa tattgctatt agatgaggtt acaacaggtt tagatgtttc tgtgcaagcg
    17521 gaagtattgg atttaataag agaaatccaa agagaattta agatttctat gatagttgta
    17581 tcccatgact ttgatgttat aaagatgctt gctgatagga caattgtaat gaaaaatgga
    17641 agtgtaattg agtctggtct tacagaccaa ataatggagg acccacaaca cccatatact
    17701 cagcaactta taaattcatt gctataaata tcgtttgtaa ataagcaaaa tgggggtttt
    17761 taataaaatg aaagaaacta tacttgaagt aaataatttt agtaaagact ttgagttaca
    17821 tgcattgaac aagactataa cggcatgtag taatataagt tttgaagttt caaaaggtga
    17881 gtttttagga ataataggta aatctggagc aggtaagtct accatattaa aaagtatcta
    17941 taaaacatat ataccaacaa ctggagaaat aatttttaat tcagaaatat atggtaatgt
    18001 agacctggct aaaattggag accgagaaat gataaatctt agaaagaaag aaataggata
    18061 tgtatcacag tttttaaaga cacttccacg aataactgca attgaactgg tagtacattc
    18121 tttaattgag agtgggtttg aaaaggattg ttcgtatgat atggcaaaag atatacttaa
    18181 ccaatttgaa ataaaggaaa atttatggga tgcgtatcca aataattttt ctggtggaga
    18241 aaaattaaga ttaaatttag ctcaagctat ggttaaaaag cctagacttt tgttacttga
    18301 tgaaccaact gcatcacttg ataatcaatc taaaatatat gtaaaagaaa aaatgttgga
    18361 gcttaaaagt caaggaacta caatgatagg aatttttcat gatatagagt ttatggaaac
    18421 tgttatagat aagaccttta ctatgacaaa agggactata agtgaaagtg gggtagccta
    18481 gatgataaaa ctaattcata atgcaaatat tgtattagaa gatatgattt tagaaaatgg
    18541 tcatttaatc atagagggag atacaataaa aaaagtatca aatgataaga ttgattacgc
    18601 atttgacttt aatgagatag atgaaataat agatgcaaaa aacttatatg taatacctgg
    18661 tattatagat attcacagtg atgccattga aaaggaaata gagccgagac caagtacatt
    18721 attaccattt aatatggcat tttatgaact agataaaaaa ctacctgcaa atggaataac
    18781 tacagtttat cattctatat ctttagggga tggagtagga gttagaagta ttgataactc
    18841 tttaaagatg attaaaaata ttgattcata taaaaatata gatagtaaga gcataaatca
    18901 taaagttcat ttaagatatg aagtgttgta ttatgaaggt ttagaaaagg ttttagaact
    18961 attagatgaa aataaaatag actacttatc aataatggac cattctccag gacaaggtca
    19021 gtatacaaat ccaacatttt ataaagagta tgctactaaa gtgtggggag ttacagaaaa
    19081 ttatgtagat acatggttag atgatttagt aaatctgcat gataatttag attggaataa
    19141 aatagctaat attataggta tagccaaaac taaaaatata aatgttgcat cacatgatga
    19201 tgatacatta gaaaaaatgg atttcataaa aaatataggc ataaatgtat ctgaatttcc
    19261 tataactctt gaagttgcaa aacattcaaa gaaattgggt gttctaactt gtttaggtgc
    19321 accgaatatt gtaagaggta aatctcataa taacaattta aaagcaatgg atgctatatt
    19381 ggaagattgt tgtgacattg tgtgttcgga ttatcttcca tcagctatga taaagtctat
    19441 gtgtatagtt gcagaaagaa taaataattt aaataaagca gtcagtttat ttacctcaaa
    19501 tcctgctaaa gcagttggta tatatgatga aagaggtagt ataaaagaaa ataaaaaagc
    19561 agatttagtt ttagtagata ttgatagtga atatccaaag gttgtaaata ctattgtaaa
    19621 tggaaaaaca gtttataaaa gggaggtata aagattgcta acagagttac attgtcatac
    19681 aagtatatca gataataatt ttactactga agaaataata aaaaaagcag cagaaaagaa
    19741 tataaatatc ttagcaataa caaatcatga cacattatct ggattggatg aagctcttgt
    19801 gcttggagag cattatggaa taacaataat accaggtata gaaatttcag cctatgatta
    19861 taaaaataga aaaagagttc atattcttgg atataacata gatttaaaca gtcaagaaat
    19921 aaaaaatttg tgcaatccaa tggtatgtga cagacataaa gcatctgtta agatggtaaa
    19981 taaaattata gatttaggat acaaaatatc tttggaagat gtaaagaaat attcatcaaa
    20041 gactggaata tttaaacaac atataatgaa agctcttata gataaaggat atacagataa
    20101 tatatattca agtctatata aagaattgtt tcatagagga aacggcaagg tatatgtatc
    20161 tttaaaatat gtggattaca gagatgcaat aaaagctata aaaaattctg gtggaatttg
    20221 tgtgttagct cacccgggtc aaatggataa ttttagcgct attgaagaga tggtaaaagt
    20281 aggattagat ggaatagaag tatatcatcc atctcacaac aaagaactag aaaaagagtc
    20341 gctttcttat gctaagaagt ataatctagt ggtcacagga ggctcagatt accacggatt
    20401 ttattctcaa cataattatg aacttggaag caagagctta aataaagaag atttatttaa
    20461 gtttaggtta gctttaggag gggtattata aatgtctttt ttacaagtaa aaaatgtagg
    20521 taaaagttat ggacaagtta aggtgttaaa agacatatct attgatattg aaaaaggaga
    20581 gtttatttgt ttactaggtc caagtggttg tggaaaaagt acactgctta gaataattgc
    20641 agggcttgag gataaacatg gtggaaaaat aataataaat gataaggata tgactaattc
    20701 accacctgaa agtagaaatt ttggtatagt ttttcaatca tatgcattat ttccaaatat
    20761 gaatgtttac aaaaacatag cctttggact agaaaataaa aacataagta aatctaatat
    20821 tgataaaaaa gttaaagaag ttctagaagt tgtagaatta agtggatatg agaaaaaata
    20881 tccatcacaa ttatcaggtg gacaacaaca aagggttgca ttagctcgtg cgattgcttt
    20941 agagccagac tttttacttc ttgatgaacc tttatctgca ttagatgcaa aagtaagact
    21001 taaacttagg gagcagatta gaagtttgca tagaaaacta ggcattacaa ctataatggt
    21061 aacgcatgac caagaagaag ctctttgttt agctgataag atggttgtta tgaatagagg
    21121 agaaataata caagttggaa cgccaaagga ggtatataaa aatccagaaa caccttttgt
    21181 agctgatttt ataggaacaa taaattttat agatgatggt attaataaga tagctattag
    21241 accagaagat ataaaagtag agtctaatag agattctaaa gataaggata taaaagtggg
    21301 agaaatttta gatatcgaat ttagaggatt taactataga attacagttg agtatcgttc
    21361 taagcagatg aaattagatg tagtttccaa agttgcagaa caaatgaaac tgtgtatagg
    21421 ttctaaaata aactttaaga tacctaaaga aggaattgta caatataaat cagagggatg
    21481 tgcttaatag ctaggggtga ataaatgata acatcaaaaa ataaaagaag aataagtgat
    21541 agcacaatac aaaaatattt cttgattttt tcggtaattg tactaatagt atttatatta
    21601 tttccattag tatcattatt aaaaaatgca tttatggata caagtggaaa ttttatagga
    21661 atttcaaact ttagtaaata tgttgaaaat ccaagtctta tagcatcatt taaaaattca
    21721 atttttgtat ctacagtctc atctttaata tcactatgtt tagcatttat atattcatac
    21781 gcaataacaa gaacaaacat aaagtttaaa gaatttttta aaactatggg aatgtttcca
    21841 ctttttgcac caactatgtt atatggtatt tcacttatat acctttttgg aaataaggga
    21901 atctttacaa ccatgggtct ggaagtacca ctatatggtc cattaggaat aattatttct
    21961 cagattctat ttacatttcc acaagctttt ttaatattat ctgtagcact ttcgatggct
    22021 gattacagac tttatgaagc agcagatagc ttgggtgcta gtgaatttaa gaaattttgc
    22081 aaaataacaa ttcctggtat aaagtactca ttacttagtt cattctttgt atcctttata
    22141 cttgcattta cagattttgg agcagctaag gtagttggag gaaactacaa tgtactggct
    22201 actgacatat ataaacaagt agttggacaa tttaacatac caatgggagc tactgtttct
    22261 atggtaatgt taattcctgt actaatagca tttacattag ataagatggc aagtaagaaa
    22321 caaggtatga ccataactgc aaagtctatg ccatataaga ttaaggaaaa taagtttaga
    22381 gatagagtat ttttaatagt atgtagtttt ataacattgc taatttttat tttaatattc
    22441 gtttcagtta taggttcagt tataaagtta tggccatata atttaagctt tacaatggaa
    22501 cactatacat ttaaaggtat tttgggaagt ggaataaaaa catatttaag cagtttgaaa
    22561 atatcaactt taacagcaat atttggaact atatttgtat tttttagtgc ttatatgata
    22621 gaaaaggtgg ataagtttcc taaattaaga caaacagctt accttttatc aatgatacca
    22681 atggccttac ctggcttagt tttaggtata tcatatataa ctttttttaa ctcagcaagt
    22741 aatccattaa attttttata tggaagtaca gcaatattag ttatagtaaa tgtagttcat
    22801 ttttattcag tttgttttat aacctcaaat tcatctttaa aaatgttgga taaagagtat
    22861 gaattagttg caaaatccat aaatattcca ttctataaag tattttttaa tataacagtt
    22921 ccaatgtcta taacatcaat acttgaaata gtagtatatt attttgtaaa ttcaatggtt
    22981 actgtttcag cattaatatt tttatataca cctcaaactc agactgcatc aatatcaata
    23041 ctaaagttag atgagatagg atatatagga ccatcagctg ctatggcagt attagtgctt
    23101 ttaacaaata taatagtaag acttttgtac gaatttgtaa caaagaaatt aaaaaataac
    23161 acacaaaaat ggcagcaaaa agatgttgca taggggaggt aaaaatgaaa tttaagaagc
    23221 tagtttcttt aggagtatta tcaacacttt tattaagtgt aataacagga tgtagtactg
    23281 attcatcagg ttcagattca aagtctgata gtaagacaaa aggggattta acagtttaca
    23341 cagcaattga agaagatagt atagagccat atttagctac atttaaagag aaatatccag
    23401 atattaaatt aaatattgtt agggcatcaa caggagatat aacagcaaga ttattagcag
    23461 aaaaggataa tcctcaagca gatgttgtat ggggagttgc agccacaagc ttattggttg
    23521 cagatgacca ggggatgtta gaaccatatg ctccaaaagg ttctgaagaa atagagtctg
    23581 catttaaaga tgataaagaa gttccttctt gggttgggat agatgcttgg atgacaggta
    23641 taacagtaaa cactaaagaa ttaagtgata agaacatccc agttcctagt tcttacgaag
    23701 attcaataaa gccagaatat aaaggtctta tatctatgcc taatccatct tcttctggta
    23761 cagggtactt aacagtatca gcccttatac aaattatggg ggaagaaaaa gcatggcagt
    23821 atatggataa gttacatgag aatattggtg tatacactca atctggttca gctccagcta
    23881 catcagcagc ttcaggagaa tatcctattg gtatatcttt tggatataga ggtataaagt
    23941 taaaagagga aggctatcca gtagaagtag tatttccaaa agaaggttct ggatgggata
    24001 tagaagcaaa tgctcttgtg aaaaaagata atataaaaga agcatcaaaa gtatttttag
    24061 attgggctat aagtaaagat gcaatgaacg aatactctaa aaactatgca gttactacta
    24121 tttctacagg aaatccaata ccagaaggat tccctaaaaa gccattagag caaatgatag
    24181 ataatgattt aaaatcagca gcaaaaaata gagaagatat actaaataaa tggatatcaa
    24241 aatatgatgg taagacagaa aaagagagtt aagtaaaact atacaaatgg agtaaaaaga
    24301 aaaatttttt ttaagtagat tttattatta taatataagt tgagaataat ataggtcaag
    24361 aaaagtgaat attctataaa aatgtataga gtaagtatac cattcagttt gaatggtata
    24421 ttttttattt ttagagatta atttagaaat agatatacta cagtttaaaa tagtatattt
    24481 taatttcatt att
//
LOCUS       DAMJVK010000056        24399 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.057, whole genome shotgun sequence.
ACCESSION   DAMJVK010000056 DAMJVK010000000
VERSION     DAMJVK010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24399
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.057"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            163..663
                     /locus_tag="PC414_002329"
     CDS             163..663
                     /locus_tag="PC414_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2464720.1"
                     /translation="MIEGIKFIKAEEKYIYSYWQTFDKIAKERKYLAMDEAFPFEETV
                     EFIKNIINKNLPQLFIIDLESDNCIGWCDVLPKTEKVGYLGMGILKEYREKGIGSSLL
                     KQIIDLSKEYGYEKIELDVFKSNSRAIHVYKSLGFVEVNTISSGFTWNDRPVKEEVIQ
                     MELTLI"
     gene            complement(913..1692)
                     /locus_tag="PC414_002330"
     CDS             complement(913..1692)
                     /locus_tag="PC414_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464721.1"
                     /translation="MNKNILNNSFPEVPESFHNSLSKTLNSLPEREENYKMINNKTHK
                     LPFRKGLVATLAITLILSTTALAVGHIYSLVGKSSTKATYTSIPTTEQVKKDFNFTPN
                     IVSEFSNGYKFKGAYTVNKKAFDKENNVLGKSKALSFEYKNGNDEISLDTANNVLGKS
                     SDNLKVVDTYKDINISYEAFAQKFIPEGYKMTEQDKIDEKSGKYTFSVGPNNESDKVE
                     VNEFKFLTWKQDGVYYSFTVQDSNLSMNDLVKMAHEVIDAK"
     gene            complement(1689..2168)
                     /locus_tag="PC414_002331"
     CDS             complement(1689..2168)
                     /locus_tag="PC414_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2464722.1"
                     /translation="MDKTTFTNNILESEQTLYRVSKSILGNDQDCEDAVNNAILKAYE
                     KLDSLKEEQYFKTWLIRIVINECNSLRRKRLKSLSFEDVFKNKKIDEKDDYSDLYTAI
                     QSLPKKIKIPIVLYYIEGYSVDEVKEILDIPQGTVKSRLSRGRRLLKTKLENTEVII"
     gene            2976..3677
                     /locus_tag="PC414_002332"
     CDS             2976..3677
                     /locus_tag="PC414_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HDF2464723.1"
                     /translation="MKFQEMINSPGLWIVSSFLVIISVVQAIVFMKEALKEASNLGIE
                     KKGIKAAIRSASVTAIGPSLSPVITLLSLVAVIGAPTTWMRLCDVGAARTELGVISLT
                     SNLSGVEVGSAAFGAEAFSYALWGMALNNLGWLFVVFILGHRMRGIVEKMNMKYNPTW
                     VKKLLAGATVGLFAYLLSNQIKTFEIPKLTPAIISAIVMLVLTTLFKKNQRLQELSLG
                     IAMLIGMFGTQIILS"
     gene            3703..4359
                     /locus_tag="PC414_002333"
     CDS             3703..4359
                     /locus_tag="PC414_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464724.1"
                     /translation="MYDKKMIKIGRISIGLAIIANFIPAIYVGIRFGQMPMLSVIFQI
                     WGLVAATYGVSWIVQPIAYYPTLGAPGSYIGWLAGSVGDIRMPAASMAQKIAGVEAGT
                     HEGEVVGTIGTCCSIFVSASMITIFTIVGYKVIPLLPEFVTNSFNYILPALFGAIYVD
                     IARKDIRAGGCTIAVALAIMYFGEKIGIPGGLLTLFIVLGGILITRVFFVIDNNKTKE
                     "
     gene            4515..6155
                     /locus_tag="PC414_002334"
     CDS             4515..6155
                     /locus_tag="PC414_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HDF2464725.1"
                     /translation="MFDVKIVNGIIVDGTGNSRYKSDVGIVGDKIMAIGDLSQKEAKE
                     TIDATGKFVSPGFIDFHTHSDLSLVYDKYTRSRIHTGVTTDVIANCGIGVAPIREEKK
                     QELIDYLGTRIIGTIPTKLELHWNTMQEYFDYLTENSPAVNVVAYVAQGPVRINEMGF
                     SKEPATLEQLKNMKLEVRKAMEAGCVALSSGLVYLPGAYTKKEEMAELCKELIPYNGY
                     YISHIRDEGDEEMEALDEAIYIAKTAGVPLHISHLKVMGHKNFGTIDEVFKKLDEAEA
                     DGLEVTFDCYPYTAGMTSLGALLPPWAFEGGVENMVKRLEVQENRDRIIKELEEGIPG
                     WQCFYQLAGGWNGVVLASVMTEANKYVEGKTLMEVAKINGENPFDTFFRLLIEEKSKI
                     QVVVHTQGQEDTDKVVCHPKSCIGSDSMDLSTEGLLSLGKPHPRAFGTFGRIFSYYVR
                     EKGMLTFEEAVKKITYLSAKRLGIYKERGLLKENYFADIVVFDPDTIEDKATYSNPKQ
                     YTVGVEYVLVNGKIALAGGKQTDVCAGRVIKNPLSIAK"
     gene            6513..7835
                     /locus_tag="PC414_002335"
     CDS             6513..7835
                     /locus_tag="PC414_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464726.1"
                     /translation="MKILYVGPGMVIDKILAVSKKNFPNIEADFLRYDRYIEVPDLLE
                     KYRGRVDAVLFTGKSPFKFFEKRQKDFVLSDYIPRHETTLYRVLLEITYLLKFDISRL
                     SIDTYDKAMMKRLYKEIGVEFKDEHMFFAEQKYLEEDYIKFVLEFHKYNYQNNDVCCC
                     ITGLEEAYHIMKKDNIPVVLALPSEDVMVQSIKNIQMRYIAKKNNENQIIVLAIKLNM
                     PSEYSLMKEDEYAYLSQRIKILDKLYHFNSRIDGVLVEQSRSEFMIFTTKKILELETK
                     KYRDIYLLDMIREVSVINTYIGIGYGKTANEAKFNAYESIKMAQSQKNDAAYVVFENG
                     EIMGPLKPNPTVKEKNSFDERFYQIATETGLSANTVYKVFNKIINSGKTEFTSKELAT
                     ICGVSIRTMDRIILRLCDSGYCEVVSEQLMSKYGRPSRILRFKPFLLF"
     gene            8032..9039
                     /locus_tag="PC414_002336"
     CDS             8032..9039
                     /locus_tag="PC414_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HDF2464727.1"
                     /translation="MGKYQDSKKVVRNYFEALENATGDEVENVLKQHMKSDYNWKGVY
                     PFREQEGTENVADVFWKPLKKSLSNMQRRQDIFIAGTNEIDNSEWVMSMGHFMGLFDN
                     DWLGIKHTRKMISLRYAEFNCVQDGKIAKTGLFVDIIGFMIQAGVNPLPPQTGSYFVY
                     PGPIDHNGLLFEDADEVEGVKTLALVNKMVDDLSELNESGAMGCPPEILEKSWAKDMI
                     WYGPGGIGASYTIPRYQEQHQLPFRNNLKGKTFNGHVCRFAEGNFACFFGWPNLTNVP
                     CGGFLGLPGGEVKADMQVVDVYYRKGDKLQENWVLIDIPYWLKQQGLDILERTKSIFN
                     A"
     gene            9077..10504
                     /locus_tag="PC414_002337"
     CDS             9077..10504
                     /locus_tag="PC414_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HDF2464728.1"
                     /translation="MASTNKNNATSKEEEKEVSTSGLESCTVKENKAKMNQTNFSNIN
                     ENQTNENKTNTYKRNIDKGNIDKGNIDKTNTNKSDINKVNIDTVDTDKLNTNTLTEKN
                     KKVIFKGEDNMSSTKIYYGDSLEVTPVGVMDYNDFSKQTKREQEIPGFDSKYRDFVDY
                     IMKITHNIWEEKGIGVIYDTYHNNVTMHCGSSNLVGIKDVISNTLQTLHAFPDRRLIG
                     QNVIWSNFGADGFLSSHRVLSTATNLGDSNFGPATGRKINFRTVIDCATTNNRIHEEW
                     LVRDNLWIVTQLGLNPQEVAKSMAKASESKVLSLQSTYGICESMDGQFMPTKYQAKDD
                     SVGEMMLEMTSRIYNYKYINEVKKYYHDNAVVHFICDKDLNGYDEIQGMIVSLFASIP
                     NGSYEVERVTCNQREKNEGYDVSVRWRLRGINEGIGFFGQPSGKHIEVMGINHYHILQ
                     GKVKEEWITFDGMDVLKQIYMGVEE"
     gene            10646..11911
                     /locus_tag="PC414_002338"
     CDS             10646..11911
                     /locus_tag="PC414_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2464729.1"
                     /translation="MKTTVEIENDIILGKDYSNLKRGIAFFSIALIYFFYCYNFMVGT
                     FIKPTMIYALADGGFGFSLKQTEEIFAVMSFGTIPGTFIFGVISTKIGKKRTLISVAL
                     LIGLTTFIPMLSPTNIMLWKIARLCTGVVLGGVFGTAMPLVADMFPSKYRGKLAAILT
                     ALFSLAMIFGGKVYGVLGDANWQILMYTAIIPPIVGAILAIFFVPDDLEYTKELVKKG
                     KETGEKISYISMYKGKYLWIGLGTILLSGANFTAYSAFSNNATTYLVTGIGMSAAVAE
                     SIYSLQGIGQLVGYLFWGSIADKFGRKIPFIGMALAAVFVFIYTKLGGDNTTTFYMIS
                     VLLGVAVGYSGAWGAYYTELFPSKYRSLSSGMSFNGGRIISTFAIPAIAGTASGTLGM
                     MTIFYISIAVFVAGAIVWLFLPETLNNKK"
     gene            11953..13551
                     /locus_tag="PC414_002339"
     CDS             11953..13551
                     /locus_tag="PC414_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HDF2464730.1"
                     /translation="MISSIDVCIIIGYLLLMLVIGYYSGKDNKNQEDYFLAGRSMPWI
                     PIALSVAATMISANGFIGGPGWAYVDGMYPVMVNITVPLAIFFALSITTPVIYKLKIT
                     SIYEYMGLRLGNYSRGLTIIQFFINSLIQASSMVYIPVLIIQMITGWSLYALVPIVVL
                     VSIIYTLLGGIKAVIWTDSIQMIVVISAVFIVIIVALKGMNLSFFDTLQIAQQSGKLN
                     TFDFSRDISVTNTFWATLIGGTVMWIRYFCFDQSQVQRVLTSKSLKCAKNSFVVSAFV
                     MNLVYYFMLLVGVILFVFYGGRTFETSNEIMITFILNELPVGAIGIIIAGVFAAAMSS
                     IDSILNSMTTVFTKDIYEFYFKKDNKESSLKVTMIITVVIGVVMTGFIIMGFGGSIKS
                     ILDLVGNYISYFAGPATGAFVLAMFTYKANDKGVSIGFIVGLVLGFFISNKYNVSWLW
                     NPAIGATITLIFGYIFSVILKKNHNADDIKKYTAFGMRNHLISQGMDKVDGVSIVPFS
                     LGKQELIVLAFFIIQYIILFLIQF"
     gene            13586..14608
                     /locus_tag="PC414_002340"
     CDS             13586..14608
                     /locus_tag="PC414_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDF2464731.1"
                     /translation="MNQKKVHYNKSATSKDVARLAGVSQSSVSRAFGSANGKGVKPEV
                     RERIFRVANEIGYVPNLVARGMISGKTNVIGLIVGDSLGPFYNRIINLFVEKIQEIGK
                     QCLVFKVPRQQSIDSIISKVIQFQVEAVIITASAMNKVMAETCEENNIPVILFNRFIP
                     GIKISTVYVDPIEGGGMAAEYLLKKGHKNIGYIQFTRETSEEMEKKIGFYSKLRQSGI
                     HNLKEESANYDYDSGYEAGKRMLALSNPPTAIFCTSDLIAIGVMDAARFEYKLRVPED
                     LSVIGYDDIQMASWKSYNLTTIRQPLDLLIEKTINILKSLLNEENPESVVEMLKPELI
                     ERGSTL"
     gene            complement(15104..16507)
                     /locus_tag="PC414_002341"
     CDS             complement(15104..16507)
                     /locus_tag="PC414_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2464732.1"
                     /translation="MKMLNNFFKKINNPAPVFAQTKMERDNQTDDSLSTKPKTTFRDV
                     AGLDEVKEELFEIVDFMKSPQKYQKMGAKIPKGVLFYGPPGTGKTLLASAVAGETNSS
                     FFNVTGSEFVEKYVGVGAKRVRTLFEKARKEAPSIIFIDEIDAVGAKRHLESNNEKDQ
                     TLNQLLVEMDGFNKDSNVLIIGATNRLDLLDEALLRPGRFDRHIHIGAPNYHTRFEIL
                     KVHTDDKPIDKSVNLELLAKKTHGFNGAHLSNIANEAAIFAVRDDSECITSEHFDKAL
                     ERVIAGLESKNSALVEKEKKIVAYHEAGHALVSDIVGICPIQKISIVPRGQALGYVLQ
                     LPDEDRYIYTKDELIGKIKILLAGKASEELIFNHKSTGAKDDLKKVTEIANQMVCEYG
                     MSNLGFMTIDGNDKTFLCDKVQKEANRIVEICYKETLEMLKDNLEDLHSVSKFLFEKE
                     TMTHEELKDLIGKEAVN"
     gene            17680..18189
                     /locus_tag="PC414_002342"
     CDS             17680..18189
                     /locus_tag="PC414_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009528606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HDF2464733.1"
                     /translation="MSKELAINEQIKDKEIRVLSPTGEQLGVMPTKEAQAMANSKNLD
                     LVQISPNANPPVCKIMDYGKFRYEQARKEKEAKKKQKTIVVKEVRLRPGIEQNDLNTK
                     ANNAIKFLKKGDKVKVELRFRGRELGHKDIGKEVMLKFLDIIKEFGEPTKAPAFEGNN
                     MVVIIDPKK"
     gene            18217..18411
                     /gene="rpmI"
                     /locus_tag="PC414_002343"
     CDS             18217..18411
                     /gene="rpmI"
                     /locus_tag="PC414_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HDF2464734.1"
                     /translation="MPKMKTHRGAAKRLKKTGTGKLKRAKAFKKHILTKKSAKTKMNL
                     RKSTLVSDGDAKRIAQLLPY"
     gene            18467..18823
                     /gene="rplT"
                     /locus_tag="PC414_002344"
     CDS             18467..18823
                     /gene="rplT"
                     /locus_tag="PC414_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HDF2464735.1"
                     /translation="MARVKKAMNARKKHKKILKLAKGFRGSRSKLYRPANTFVMKALK
                     NAYIGRKLKKRDFRKLWIQRINAAARMNGISYSRLMNGLKLSGVEVNRKMLSEMAIQD
                     PEGFAKLAEVAKAKLA"
     gene            19133..19678
                     /locus_tag="PC414_002345"
     CDS             19133..19678
                     /locus_tag="PC414_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464736.1"
                     /translation="MNEKLMDCFVNPIKCKLLIEIFSNNRTTAKQLGEKFQDISKATL
                     YRYLNKMLKDDIIKVVEENKIRGTVEKVYAMKLDIADDVNKIIDENSGEEYAQLFTQY
                     MMGFLREFYEYAERKEKDILKDGSGFSVCPVYASIDEIVEVSKKIASILEPLRNNSHE
                     EGRRMHSIGVIITPPKKDEGK"
     gene            19680..19771
                     /locus_tag="PC414_002346"
                     /pseudo
     CDS             19680..19771
                     /locus_tag="PC414_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002605025.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
     gene            20113..22038
                     /locus_tag="PC414_002347"
     CDS             20113..22038
                     /locus_tag="PC414_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF2464737.1"
                     /translation="MKIKFVIMPLVIFLMVLFVGCQSTKDTEVKAKDTEIKILGTGDL
                     HSILTDSMVSYVNEEREKNKNLLMVDAGDFYGTQSREMWEWSSGKKLINIKRNGRAEY
                     DDIIKSSKDEVPIVKDMAKLKYDAVVLGDNEFVSNDKQSLDKLVSDFKNNNMPLVSAN
                     IYEQSGENYVQPYVMKNIKTDEGNVKVGILGLTIKEVGETLGLEDFEKDKKARELGEQ
                     ADYKGKLYANDLVEDAKKWIKVMEKESPDIIVAVVHSGEEPKTDRNPGNRIKELATTV
                     DGIDAIVAGHTHEKIDEHKYKNKSGDEVIVTQPGWHGDRVSEINFKLNKENGKWDIKE
                     KSSKLKIIDKEVSIVATADLHSHFSDKLTVDLVNERSNPIEPVVVDAGDFLDPQTDEM
                     TQWYKEWKSIRDNNSDKTISRCPMVMDMNQGMYDAVVLGNHEFVSEKDEFWSDERLLD
                     AVIEDFEQVAISVLSANIYRQSGKNYVKPYIIKDVETSEGNVKVGILGLTIKEVGKGL
                     KNVNLQEQFQYKDKLYANDLVEDAKKWVKVMKKKKPDIIVAVVHSGEEPKNPKHPGNR
                     IKELATTVDGIDAIVASHTHEKIDEHEYKNKSGEKVIVTQPGEHGKYYSKITFKVNKE
                     KGSWTINDKFSKTIEVE"
     gene            22439..24343
                     /locus_tag="PC414_002348"
     CDS             22439..24343
                     /locus_tag="PC414_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF2464738.1"
                     /translation="MKIKFIIVASIIFLIVLLVGCQSTKDTEINVLATSDLHSIIPDN
                     LISYVEEERKYDENLLMVDAGDFFDMQSGEMNKWFTGQKLVRFKDGIPEYKKIAEPRE
                     GEVPLAKKMAKLKYDAVTFGNHEFVSNDKQSLDKLVSDFKNNNIPLVSANVYEQSGEN
                     YVKPYVMKNINTDKGTVKVGILGLTIKEVGERSRFKSFEQDKKARELGEQPQYKGKLY
                     ANDLVEDANKWVNVMEKKEKPDIIVAIVHSGEEPKNPKNPGNRIKELATTVEGIDAIV
                     AGHTHEKIEQHTYKNNSGEEVIVTQPGAHGNSISKINFKLDKKNDKWFIKDKYSELKV
                     FGKEVNIITTSGLNPKFSDEMAVNLFNERDRDVQPIFIDTGDFLNTNTKEMTKWAKEW
                     QYNENKGIYKKINEWPIVYLGYDAVVLGSHEFTEDKETFEEKKSTLDYIIENFEEMKI
                     PVLSANIYDESGENYVKPYIIHNVETREGNIKVGVLGLTTTDKDEEDLSNSSLSKDSK
                     TSKIPEETGKLYTNNLVKDASEWVKVMQKESPDVIVVVVHSDNNSTLKNPSSEVKKLA
                     MSVDGIDAIVVGHTKNKVEEHIYKNKSGDEVIVTQSGEDCYSKISLELRKENGKWNII
                     NKYSKAIKMK"
BASE COUNT         9131 a         2719 c         4449 g         8100 t
ORIGIN      
        1 ttattttaat agagatgcaa gagatagata tattggtttt aattttatag aaatatacaa
       61 agatatttta agctaatata tagtaattta tatagcatta taaactagaa tcatataaaa
      121 taagttaact acaatcaaaa attaaaaagg ggaaatgtta agttgataga aggcataaaa
      181 tttataaagg cagaagaaaa gtatatttat tcgtattggc aaacttttga taaaatagca
      241 aaagagagga aatatctagc tatggatgaa gcctttcctt ttgaagaaac agttgaattt
      301 ataaaaaata ttataaataa aaacttacca caactgttta taattgattt agaatcagat
      361 aactgtattg gttggtgtga cgttttacct aaaactgaaa aagttggtta tttaggcatg
      421 ggtattctta aagagtatag ggaaaaaggt atcggaagta gtcttttaaa acaaattatt
      481 gatttatcaa aagaatatgg gtatgaaaaa attgagcttg atgtatttaa aagtaatagt
      541 agagctattc atgtttataa atctttaggg tttgtagagg taaatacaat atctagtggt
      601 tttacatgga atgatagacc tgtaaaagaa gaagtaattc aaatggagct tacacttata
      661 taaaataaag ttaaagtctt taaaattcca tatacacttg tataaaataa agttaaagcc
      721 cttaaaattc catataaaag aaatattttt aagggctttt taataatgct tcttttgaag
      781 gtagctaaat atctaaaatt ttatatatta aatttaaaga aaatataaag atggaagata
      841 caaccatgtt tactaataaa aaaattaaag tcataaataa taaactcata aacaacttat
      901 tattacttat gtttattttg catctattac ttcatgagcc attttaacta agtcattcat
      961 agataaattt gagtcttgaa ctgtaaaaga atagtaaact ccatcttgtt tccatgtcaa
     1021 aaacttaaat tcattaactt caactttgtc tgattcatta tttggcccca cactaaatgt
     1081 gtatttgcct gatttttcat caattttatc ctgttctgtc attttataac cttctggtat
     1141 aaacttttgt gcgaatgctt cataagaaat attaatatct ttgtaagtat ctacaacttt
     1201 tagattgtca gaactcttcc caagtacatt atttgctgtg tcaagagata tttcatcatt
     1261 gccattttta tattcaaaac taagtgcttt tgattttcct aaaacattat tttctttgtc
     1321 aaatgctttt ttatttacag tatatgctcc tttaaattta tatccattac taaattcaga
     1381 aactatatta ggggtaaagt taaagtcttt ttttacttgt tctgtagttg ggatagatgt
     1441 ataggtagcc ttagtacttg attttccaac taaactatat atatgtccta ctgcaagggc
     1501 tgttgtgctc aatatcaaag taatggctaa agttgcaact aagccttttc taaatggtaa
     1561 tttgtgtgtt ttattattaa tcattttata attttcctct ctttctggta aactatttag
     1621 tgttttgctt aaactattat gaaaactttc tggaacttct ggaaacgaat tatttaatat
     1681 atttttattc atatgattac ctctgtgttt tctaatttag tttttaataa tcttcttcct
     1741 cttgataatc tactttttac agttccttga ggtatatcta atatttcctt aacttcatct
     1801 actgaataac cttcaatata gtaaaggact attggaattt taattttttt aggtagactt
     1861 tgaatagcag tatacaaatc gctatagtca tctttttcat caattttttt gtttttgaag
     1921 acatcttcaa atgatagaga ttttaatcgt ttgcgtctta atgaattaca ttcatttatg
     1981 actattctaa tcagccatgt tttaaagtat tgttcttcct taagagaatc caacttttca
     2041 tatgctttta aaatagcatt gttgacggca tcttcacagt cttggtcatt tcctagaata
     2101 gacttagata ctcgatacaa agtctgttct gattcaagta tattgtttgt aaatgtagtt
     2161 ttatccaact aagttccttc ccttctatgc aaatcgtagc gctacttttt tgcctttcac
     2221 ttattagata aaattttact atagatggtt cacttttttt atgattattt gataaattta
     2281 agtttgaaag ttttgcctat tatatacaag tattctataa tatatttttt ctagtatttt
     2341 tcctgtcata tataaagcca tgtagatata ttaaacttta gaagccattt atgtatttag
     2401 ttttatagta agtctttata ttgagtaatt agaaaatagt aagtatttaa tgttgagtag
     2461 ttagaataac aatgcttttg gattatttac ttcatgtaca gatttaaaat aagttcttta
     2521 aataatgagt aaagataaca gtatcttgac taaaagtagg cttttaagaa aaaacaatga
     2581 aacttaaatg agaattaaga gttagaaatg tcattatttt agatagagat gaacataaat
     2641 aaacttaaaa aatataaatt ataaaaataa taattttttt taaaataata aagttttttg
     2701 attaaaattt ttatattgaa atgtcaaaat aataaaagac cttataagtt aattacaggt
     2761 taaaaaaata atatgtgaat tttctgatat ttactaatta tgaataaaat atttaagtat
     2821 taatgttttt agtgagactt taagacagat aatctcctat taaagatact atatcttaga
     2881 taaaataaat taaaacaata taaaaacata acaagattac agaaagaaag ctcacaataa
     2941 aatgctgaaa aatttatttt aaggaggaat aaagtatgaa gtttcaagaa atgattaata
     3001 gcccaggtct ttggattgtt tctagttttt tagtaattat ttcagttgtt caagcaatcg
     3061 tatttatgaa agaggcacta aaagaggctt caaatcttgg tattgaaaag aaaggaatta
     3121 aagcagcaat tcgctcagct agtgttacag ccattggacc ttctctatct cctgttatta
     3181 cattattatc actggtagct gtgattggcg ctcctacgac ttggatgagg ctctgtgatg
     3241 tcggagcagc tagaacagag cttggagtaa tatctcttac atccaattta tctggggtag
     3301 aagtaggtag tgctgcattt ggagcagaag ctttttcgta tgcactatgg gggatggctt
     3361 taaataactt aggttggcta tttgttgtat ttattttggg tcatagaatg cgtggaattg
     3421 ttgaaaagat gaacatgaag tataatccta cttgggttaa aaaactactt gcaggtgcta
     3481 cagttggatt atttgcttac cttctttcaa atcaaataaa aacatttgaa ataccaaaac
     3541 ttacaccagc aattatttca gctatagtta tgctagtatt gacaacttta tttaaaaaga
     3601 atcaacgatt acaagaatta tccttaggta ttgccatgtt aattggtatg tttggtacac
     3661 agattatatt atcgtgatta aaacggataa gaggtgataa ttatgtatga taaaaagatg
     3721 ataaaaatag ggagaatttc tataggtctt gcaattattg caaactttat tcccgctatc
     3781 tatgtaggta ttcgctttgg tcagatgcca atgctttctg ttatatttca aatttggggg
     3841 ttagtcgctg cgacttatgg ggttagttgg attgttcaac ctatagctta ttatcctaca
     3901 ctaggagctc caggttctta tataggttgg cttgcaggtt ctgttggaga tatacgtatg
     3961 cctgcagcat caatggcaca gaaaattgca ggtgtagaag caggaactca tgagggtgaa
     4021 gttgttggaa caattggtac atgttgttca atctttgtat cagcttctat gattaccatt
     4081 tttaccattg taggctataa agtaattcct ttgttacctg aatttgttac taattcattt
     4141 aattatatac ttccagcatt atttggagca atttatgttg atattgcacg taaggatatt
     4201 cgagctggtg gatgtacgat tgctgtagcg ttagcaatta tgtactttgg tgaaaaaatt
     4261 ggtataccag gtggattatt aacattgttt attgtgttag gtgggatact tatcacaagg
     4321 gtcttctttg taattgataa caataaaaca aaagaatagg attgcttatt aaggagaaag
     4381 actagaatta tttgttgaga aaaagaatag gattgtttat taaggagaaa gactagaatt
     4441 atttgttgag aaaaaagaat aggattgttt attaagaaat atgaatataa ataagtcttg
     4501 aaaggagaaa aagcatgttt gatgttaaaa ttgtcaatgg aataatcgtt gatggtacag
     4561 gaaattcaag atataaaagt gatgtaggga ttgtaggaga caaaatcatg gctattggag
     4621 atttaagtca aaaagaggct aaagagacta tagacgcaac tggaaaattt gtatcacctg
     4681 gttttataga tttccatact cattctgatt tatctcttgt gtatgataaa tacacaagaa
     4741 gccgtatcca tacaggtgta acaacagatg ttattgcaaa ctgtggtata ggagttgctc
     4801 cgataagaga agagaagaag caagagttaa ttgattatct tggaactagg ataattggta
     4861 caattccaac aaaattagag cttcattgga atacaatgca agaatacttt gattacttaa
     4921 ctgaaaattc tccagcagtc aatgttgtag cttatgtagc acaaggacct gtacgtatca
     4981 atgaaatggg attttcaaag gaaccagcta ctttagagca acttaaaaat atgaagttgg
     5041 aagttagaaa agctatggaa gctggatgtg tagctctttc atcaggatta gtttatttac
     5101 caggagcata cacaaagaaa gaagaaatgg ctgaattatg taaggaattg ataccataca
     5161 atggatacta tatatctcat attagagatg aaggtgatga agaaatggaa gctcttgatg
     5221 aagcaattta tatagctaag actgcaggag ttcctctaca tatttctcat ttaaaagtta
     5281 tgggtcataa gaattttgga actattgatg aggtatttaa aaagctagat gaagctgagg
     5341 cagatggttt ggaagtaact tttgattgtt acccatatac agctggaatg acatctttgg
     5401 gtgcactttt acctccttgg gcttttgaag gcggtgtaga aaatatggta aaacgattag
     5461 aagtacaaga aaatagagac agaattataa aagagttgga ggaagggatt cctggatggc
     5521 aatgtttcta ccaattagca ggtgggtgga acggtgttgt actagcctct gtaatgactg
     5581 aagcaaataa atatgtagag ggtaaaactt taatggaggt agccaaaatc aatggtgaaa
     5641 atccatttga tacgttcttt aggcttttga ttgaagaaaa aagtaaaatt caagtagttg
     5701 ttcatacaca aggtcaagag gatacagata aggttgtttg ccatccaaag tcatgtatag
     5761 gttctgatag tatggacctt tctacagaag gtttactttc tcttggaaag cctcatcctc
     5821 gtgcatttgg tacttttgga cgaattttct cttactatgt tagagaaaaa ggtatgttga
     5881 cttttgagga agcagttaaa aagataactt atctttcagc aaaacgctta ggcatttata
     5941 aagaaagggg tctacttaaa gaaaattatt ttgctgacat tgtggtattt gacccagata
     6001 caatagaaga taaagcgact tatagtaatc ctaaacagta tacagtggga gtggaatatg
     6061 tgttggtgaa tggtaaaatt gcattggcag gaggaaagca aactgatgta tgtgctggac
     6121 gtgtaattaa aaatcctctt agcatagcaa aatagaatac attaagaact tttataaaaa
     6181 tagtctttct ttgaatatta aggaaggcta tttttgttaa attaataaag attactctac
     6241 ttgtgttttc taaagaattt aactttaagt agaaaaaaca atatattaat tgtaaaatgt
     6301 aattaatatt aatgaataag atttaaatac aaaaatttaa atacaaaaat gtttcaacag
     6361 atagtcgtaa ttaaaaaact taaagtaact ttttatatta ggtataattg attattcaaa
     6421 attctatgaa tcaggagtcc tgtggcttta gtcatggtag gtttagaaaa tgttttaaga
     6481 gataatagta tcattaggaa aggagagacg aaatgaaaat actttatgta ggaccaggta
     6541 tggtaatcga taaaatacta gcagtaagta aaaagaattt tcctaatatt gaagcagatt
     6601 ttcttagata tgatagatat attgaagtac ctgatttatt ggaaaaatat agaggtagag
     6661 tggatgcagt tctttttaca ggtaagtcgc catttaaatt ttttgaaaaa agacaaaaag
     6721 attttgtatt gtctgattat attcctagac atgaaacaac attgtacaga gtgttgcttg
     6781 aaattacata tttgcttaaa ttcgatatta gtagacttag tatagatact tatgataagg
     6841 ctatgatgaa gagattgtat aaagaaattg gagttgaatt taaggatgaa catatgtttt
     6901 ttgcagagca aaagtattta gaagaggact atataaagtt tgtgcttgaa tttcataaat
     6961 ataattatca aaataatgat gtatgttgtt gtattacagg tcttgaagag gcctatcata
     7021 taatgaaaaa agataatatt ccagtagttt tagccttgcc ttctgaggac gtaatggtgc
     7081 aaagtataaa aaacattcag atgcgttata ttgcaaagaa aaacaatgag aatcagatta
     7141 ttgttcttgc tattaaactg aacatgcctt cagaatattc actaatgaag gaagatgagt
     7201 acgcttatct ttcacaaagg attaagattc ttgataaact ataccatttc aatagccgta
     7261 tagatggtgt tttggtagag cagagtagaa gtgaatttat gatttttaca actaaaaaaa
     7321 tacttgagtt agaaacaaag aaatacagag atatttattt gttggatatg attcgtgaag
     7381 tttcagttat aaacacttat ataggaattg gttatggaaa aacagcaaat gaagctaaat
     7441 ttaacgctta tgagagtatt aagatggctc aaagtcaaaa aaatgatgca gcatatgtag
     7501 tttttgaaaa tggagaaatt atgggaccac taaagccaaa tcctactgtc aaagagaaaa
     7561 atagttttga tgaacgtttt tatcaaattg ctacagagac agggctaagt gctaatactg
     7621 tatataaagt attcaataaa ataataaata gtggaaaaac tgaatttaca tcaaaggaat
     7681 tagctactat atgtggtgta agtattagaa ctatggacag gattatactt agattgtgtg
     7741 attctggtta ttgtgaggta gtgagtgaac agcttatgag caagtatggt agacccagta
     7801 gaatattacg ttttaaacct tttctgttgt tttaaataga tataagtcaa aaattaaatt
     7861 tttatgataa cattttcttt acaatttttt agatatatgt taatatttag aaaaatgcat
     7921 atgtattcat aaaataaact taaaacagct aaaaaatcat taaaaaggaa atcgtatgca
     7981 tgaaatgaga aagttaattt ataacttaaa taaatctggg aggagataat tatgggaaaa
     8041 tatcaggatt caaaaaaagt tgtaagaaat tattttgaag cattagaaaa tgctactggg
     8101 gatgaggttg aaaatgtctt aaagcaacat atgaaatcag attataactg gaaaggtgtg
     8161 tatccatttc gtgaacaaga aggcactgaa aatgtagcag atgtattttg gaagccttta
     8221 aaaaaatcat tgagtaatat gcaaagacga caagatattt tcattgcagg aacaaatgag
     8281 attgacaata gtgaatgggt aatgagtatg gggcatttta tgggactttt tgataacgat
     8341 tggcttggca ttaaacatac tcgtaaaatg attagtttaa gatatgcaga atttaactgt
     8401 gttcaagatg gtaaaattgc taaaactgga ttatttgtag atataattgg gtttatgatt
     8461 caagcaggag ttaatccatt gccaccacaa acaggttcat attttgtata tccagggcca
     8521 attgaccata atggattact ttttgaagat gcagatgaag ttgaaggagt aaaaacttta
     8581 gcattagtaa ataaaatggt agatgattta tctgaactaa atgaaagtgg tgctatggga
     8641 tgcccaccag aaatacttga aaaatcttgg gctaaagata tgatttggta tggtccaggt
     8701 ggaattggtg cttcttatac tatacctaga tatcaagagc aacaccagct accatttaga
     8761 aataatttaa aaggaaagac ttttaatggg catgtatgta gatttgcaga aggtaatttt
     8821 gcttgtttct ttggatggcc taatctgact aatgtaccat gtggaggttt tttaggatta
     8881 ccaggtggag aggttaaagc agatatgcaa gttgttgatg tttattatcg taagggcgat
     8941 aaacttcaag aaaactgggt gttaattgat attccttatt ggttgaaaca acaagggctt
     9001 gatattcttg agagaactaa aagtattttt aatgcttagt tatgcatacg tattctaaaa
     9061 caaaggggga tataaagtgg catctaccaa caaaaataat gcaacaagta aagaggaaga
     9121 aaaagaagtt tctacaagtg ggcttgaatc atgtacggtt aaagaaaaca aagctaaaat
     9181 gaatcaaact aattttagta atataaatga aaatcaaaca aatgagaata aaacgaacac
     9241 atataaaaga aatatagata aaggaaatat agataaaggg aatatagata aaacaaacac
     9301 aaataaatcc gacataaata aagttaacat agatacagtg gatacagata aattaaatac
     9361 aaatactctt actgaaaaaa ataaaaaagt tatatttaag ggggaagata atatgtcatc
     9421 tacaaaaatt tactatggag attcattaga agtaacgcct gttggagtaa tggattacaa
     9481 tgatttttca aaacagacaa aaagggaaca ggaaatacct ggatttgatt ctaagtatag
     9541 agattttgta gattatatta tgaagattac acataatatc tgggaagaaa aaggtatagg
     9601 tgttatttat gatacatatc acaataatgt tacaatgcat tgtggctctt caaatttagt
     9661 tggtattaaa gatgttattt ccaatactct tcaaacatta catgcgtttc ctgatagaag
     9721 attaattggt caaaatgtaa tatggtctaa ctttggtgct gatggttttt tatcttctca
     9781 ccgtgttttg tctacagcaa ctaacttagg tgatagtaat tttggaccag caacaggtag
     9841 aaaaataaac tttagaactg taattgattg tgccacaact aacaatagaa tccatgaaga
     9901 atggttggtt agagataatt tatggattgt tactcaatta ggattaaacc ctcaggaagt
     9961 agcaaagagt atggcaaagg caagtgaatc taaagtgctt agtttacaat caacttatgg
    10021 tatctgtgaa tctatggatg gtcaatttat gccaactaag tatcaagcaa aagatgattc
    10081 tgttggcgaa atgatgcttg aaatgacaag ccgtatttat aattataaat atataaatga
    10141 agttaaaaaa tactatcatg ataatgcagt agtacatttt atatgtgata aagatttaaa
    10201 tggctatgat gagattcaag gaatgattgt aagtttattt gcatcaattc caaatggaag
    10261 ttatgaagtg gaaagagtaa cttgtaatca aagagaaaaa aatgaaggtt atgatgtatc
    10321 tgtaagatgg agattacgtg gaataaatga aggtatagga ttttttggac agccttctgg
    10381 aaagcatata gaagtaatgg gaatcaatca ttatcatata ctacaaggta aagtaaagga
    10441 ggagtggata acttttgatg gtatggatgt attaaaacaa atttatatgg gagtagaaga
    10501 ataaagctgt ttagagtata aatagttttg aaatatatag ggaagtattg ctttataaaa
    10561 tttaagtggg gaatgtatat gaaaaataga tgtttaaact tttagcaaaa ttatgactaa
    10621 atttaaaagg gggaattata aaaatatgaa aacaacggtt gaaatagaaa atgatatcat
    10681 attaggaaaa gactattcaa acctaaaaag aggaattgca tttttttcaa ttgctttaat
    10741 atactttttt tattgctaca attttatggt gggtactttt attaaaccaa caatgattta
    10801 tgctttagca gatgggggat ttggtttttc attaaaacaa acagaagaaa tatttgcagt
    10861 tatgtctttt ggtacaattc caggtacatt tatttttggg gtgatttcta caaaaattgg
    10921 gaagaagagg actttaatta gcgttgcttt actgattgga ttgacaacat ttataccaat
    10981 gttatctcct acaaatatta tgttatggaa aatagcacgt ttgtgtacag gagtagtgtt
    11041 gggaggcgta tttggaacag ctatgccttt agttgcagat atgtttccaa gtaaatatcg
    11101 tggtaagtta gctgctatat taactgcttt attttcatta gcaatgattt ttggtgggaa
    11161 agtatatggc gttttaggtg atgcaaattg gcaaatattg atgtatactg cgattatacc
    11221 acctatagtt ggagctatct tagcaatctt ctttgtgcca gatgatttgg agtatacaaa
    11281 ggaattagtt aaaaagggaa aagaaactgg tgaaaagatt agctatataa gtatgtataa
    11341 gggaaaatac ttatggattg gattgggaac gattctttta tcaggtgcaa attttacagc
    11401 atattcagca ttttcaaata atgcaactac atacctagtg acaggaatag gaatgtcagc
    11461 agcagtggct gagtctatat atagtttaca aggaattggt caattagtag gatatttatt
    11521 ctggggttca attgcagata aatttggaag aaaaatacct tttataggta tggcattagc
    11581 agcagtattt gtttttatat atacaaaatt aggtggagat aatacaacaa cattctatat
    11641 gatatcagta ttactaggtg ttgctgttgg atattctgga gcatggggag catactacac
    11701 agagttgttc ccaagtaaat atcgttcgtt gtcatctggt atgtcattta atggaggaag
    11761 gattatatct acgtttgcaa tacctgctat agctggtaca gcttctggta ctttaggtat
    11821 gatgactatc ttttacatat caatcgcagt ctttgtagca ggtgctattg tatggttatt
    11881 cttaccagag acgttaaata ataaaaaata aaatactagg aatttatttt gttacttaga
    11941 aaggggtggc ttatgatttc atcaattgat gtatgtatta taataggata tttgctatta
    12001 atgttagtta ttggttatta ttcaggaaaa gataataaaa atcaagaaga ttatttcttg
    12061 gcaggtaggt caatgccatg gattccaatt gctttatcag ttgcagctac tatgattagt
    12121 gcaaatggct ttataggtgg tcctggatgg gcatatgtag atggaatgta tccagtaatg
    12181 gttaatatta cagtaccttt agcaatattt tttgcactga gcattaccac cccagttatc
    12241 tataaattaa aaataacctc tatatatgaa tatatgggac ttcgtttagg aaattattca
    12301 agaggattaa ctattattca attttttatt aattcattga ttcaagctag ttctatggta
    12361 tatattccag tgcttattat tcaaatgatt actggctggt cactttatgc tttagtccca
    12421 atagttgtac ttgtatcaat tatatacact ctattaggtg ggattaaggc agttatatgg
    12481 actgatagta ttcaaatgat agtagtaata agtgctgtat ttatagtaat tattgtagcc
    12541 ttaaaaggaa tgaatctaag tttctttgat acattacaaa ttgcacaaca gagtggaaaa
    12601 ttaaatacat ttgatttcag tagggatatt tcagtgacaa atactttttg ggctacttta
    12661 attggtggaa ctgtaatgtg gataagatac ttttgttttg accaatctca agtacaaaga
    12721 gttttaacat ctaaatcttt aaaatgtgca aaaaattctt ttgtagttag tgcatttgtt
    12781 atgaatttag tttactactt tatgctattg gtaggagtaa ttttatttgt tttttatggg
    12841 ggaaggacat ttgaaacttc aaatgaaata atgattactt ttattttaaa tgaacttcca
    12901 gtaggagcca ttggaattat tattgcaggt gtgtttgcag cagcaatgtc tagtatcgac
    12961 tctatattaa acagtatgac aactgttttt acgaaggata tttatgagtt ttatttcaaa
    13021 aaagataaca aagaatcatc attaaaagtt acaatgatta taactgtagt tataggagtt
    13081 gttatgacag gttttattat tatgggattt ggaggaagca tcaaatcaat tcttgacctt
    13141 gttggtaact atatctcata ctttgctgga ccagctacag gtgcatttgt actagcaatg
    13201 tttacatata aagctaatga taaaggagta tctattggtt ttattgtagg gttagtttta
    13261 ggatttttta tttctaataa atataatgtg agttggttgt ggaatccagc aataggtgct
    13321 acaataacat tgatatttgg atatattttt agtgtcattt taaagaaaaa tcataatgca
    13381 gatgatatta aaaaatacac tgcatttggt atgagaaatc accttatttc tcaaggaatg
    13441 gataaagtag atggagtatc tatagtgcca ttttctttag gtaaacaaga attaattgta
    13501 ttagcttttt ttattatcca atatattata ttatttttga tacaatttta agaagaaata
    13561 ctagatattt aaggagaaat actaaatgaa tcaaaagaaa gttcattata ataaatcggc
    13621 tacctcaaaa gatgttgcaa gattggcagg agtatctcaa tcatctgttt caagagcatt
    13681 tggtagtgca aatggaaagg gcgtaaaacc tgaagtaaga gaaagaattt ttcgtgtagc
    13741 aaatgaaata ggatatgttc ctaatttggt agcacgggga atgattagtg gaaaaactaa
    13801 tgttatagga ttgatagttg gtgatagtct aggtccattt tacaatagaa ttatcaattt
    13861 atttgtagaa aaaattcaag agataggtaa acagtgtctc gtttttaaag ttcctagaca
    13921 acaaagtata gatagtataa tttcaaaagt aattcagttt caagtagaag ctgtgattat
    13981 tacagcttcg gccatgaata aggtgatggc tgagacttgt gaagaaaaca atattcctgt
    14041 gattcttttt aatcgtttta tacctggtat aaaaataagt acagtctatg tagacccaat
    14101 tgaaggtggg ggaatggctg ctgaatatct gcttaagaaa ggtcataaga atataggata
    14161 tattcaattt acgagagaaa caagtgaaga aatggaaaag aaaattggat tttactctaa
    14221 gttaagacaa agtgggattc ataaccttaa agaagaatct gcaaactatg attatgattc
    14281 tggatatgaa gcaggtaaga gaatgttagc attatcaaat cctccaacag caattttttg
    14341 cacaagtgac ttgatagcta ttggtgtaat ggatgctgca aggtttgagt acaagttgag
    14401 ggtgccagag gatttatcag tgataggata tgatgatatt caaatggctt catggaagag
    14461 ttataattta acgacgatta gacagccact agatttgttg attgaaaaaa caattaacat
    14521 acttaagtcc ttattaaatg aagaaaatcc agagtcagtc gttgaaatgt taaaacctga
    14581 attgattgaa agaggttcta cactttaagt ggtagatatt taattaagtg gtagatattt
    14641 aattaagtgg tagatattta attaaatggc agatatttaa tcaagtgata gatacctaat
    14701 taagtgatag atatttaatc aagtgataga tacctaatta agttgtagat atctgataag
    14761 aaatttttaa gttttcaatc gttctaaatt gtgagtataa aaatctacta aatttagtta
    14821 tgtaaaatat aaattataag tgtagaaact aggctactag cttttatacc acttcaaaca
    14881 ttactggagg tagttaagtt tacagcagtt tagttttatg tagatataat attctgtaaa
    14941 tagacaaaat taaaatatgt aattaaaata tgtaagtgtc tcaaaatgaa atttttagtt
    15001 tatgaggcac ttttttgtat aaaatcaaat gtatttttat aactttaaca ataaaaagag
    15061 gcttatctct attttgagac agcctcaaat ttttaaaact atattagttt acagcttctt
    15121 ttcctatcaa atcttttaac tcttcatgag tcatagtttc tttttcaaat aagaatttag
    15181 aaacagaatg taaatcttca agattatcct ttaacatctc taaagtctct ttataacaaa
    15241 tttcaactat acgatttgct tccttttgaa ccttatcaca taagaaagtc ttatcatttc
    15301 catcaatagt cataaatcct aagttactca taccatattc acaaaccatt tggttagcta
    15361 tttcagtgac tttctttaag tcgtcctttg caccagtaga tttatgatta aatattaatt
    15421 cttctgaagc ctttccagca agtaatattt ttatttttcc aatcaactca tccttagtat
    15481 aaatatatct atcttcatca ggtaattgaa gaacataacc taatgcttgt cctctaggaa
    15541 ctatagatat tttttgtata ggacaaattc ctactatatc acttacaaga gcatgtccag
    15601 cttcatgata tgctactatt tttttctctt tttcaaccaa tgcagagttt ttagattcta
    15661 gccctgcaat aactctttca agtgccttgt caaaatgttc tgatgtaata cactcactgt
    15721 catctctaac tgcaaatata gctgcttcat ttgctatatt tgaaagatga gccccattga
    15781 aaccatgagt ttttttagct aaaagttcta gatttacaga tttgtctata ggtttgtcat
    15841 ctgtatgtac ctttagaatt tcaaatctag tgtgatagtt tggtgcacct atatgaatgt
    15901 gtctgtcaaa tcttcctggt ctaagtaaag cttcatctaa taagtcaagt ctattggtag
    15961 cacctattat taatacattg gaatctttat taaatccatc catttcaact aaaagttggt
    16021 ttagtgtttg gtctttttcg ttgttacttt caagatgtct ctttgctcct acagcatcta
    16081 tttcgtctat aaatattata cttggagctt cttttctagc tttttcaaat agagttctaa
    16141 ctctttttgc tcccacacct acatattttt caacgaactc tgaaccagta acattgaaaa
    16201 atgatgagtt tgtttcacca gctacagcag atgcaagtag ggttttacct gttcctggag
    16261 gaccataaaa taatactcct ttaggtattt ttgcacccat tttttgatat ttttgtggtg
    16321 acttcataaa atcgactatt tcaaatagtt cttcttttac ttcgtctaat ccagcaacgt
    16381 ctctaaatgt agtctttggt tttgtagaaa gtgaatcatc tgtttgatta tctctttcca
    16441 tttttgtctg tgcaaagaca ggagctggat tgttgatttt cttaaaaaag ttgtttagca
    16501 ttttcatatt cacacctcag gtaattaatt attgttaatc aattttatat tttccatatt
    16561 tactattttt tattatttat ttttcatgga aaaaatatac atgttatcta aataaaaata
    16621 aatttgtaag atgtatgaaa aaatgaattt atgtatatag agatttctac ttatgtattg
    16681 aataaagtat tacaaattat aaattatgta ttgattatat attgagtaaa gtattataaa
    16741 gcatgtattg attaagtatt tattaagtat tgaataaaat attacaaatt tagttgaaat
    16801 gaaaatgata aaaaatgttg tactatattt tacaaatcat ataaatagag aaattgttgt
    16861 agcgcaaagt agtaggtagt atattagaat ataattatag gatttgttgt gtagtatagt
    16921 agcatattag aatataatat aggatttgtt atgtagtata gtagcatatt agaatataat
    16981 ataggatttg ttatgtagta tagtagcata ttagaatata atataggatt tgttatgtag
    17041 tatagtagca tattagaata taatgtagga cttgttatgt agtatagtaa catattagaa
    17101 tataatgtag gatttgttat gtagtatagt agcatattag aatataatgt aggacttgtt
    17161 atgtagtata gtaacatatt agaatataat gtaggatttg ttatgtagta taatagtata
    17221 ttagagtaca atatagaatt tgttatgtag tatagtagta tattagaata caatatagaa
    17281 tttgttgggt agtatagtgt tatattagaa tacaatatag gatttgttat gtagaatata
    17341 tagagtcatg tagaatatac atatataaaa ggattttggg tataaattta aaaattaata
    17401 gcaaaatgag tagtatagac atataattag tagtaggaag taattaaatt tttcctaaac
    17461 aaaaaaaata gttgcaaata atttgattaa gtgatatagt aaataaggta agaataaaac
    17521 aaagaagaag tccgcttctc accttacgcc ggaggttagt aaggtaaata taagttaaag
    17581 cttaatttta atttaagttt gtttaaattt ggtaacttat attgcggatg attacgtcat
    17641 ccgcttttta ttttataaaa attttggagg tgtcctacaa ttagcaaaga attagcaatc
    17701 aatgaacaaa taaaagataa ggaaataaga gtcctttcac caacaggtga acaacttgga
    17761 gtaatgccta ctaaagaagc tcaagcaatg gctaatagca aaaacttaga tttggttcaa
    17821 atctcgccga atgcaaatcc accagtatgt aagataatgg attatggaaa gtttagatat
    17881 gagcaagcga gaaaagagaa agaagctaag aaaaaacaaa aaactatagt tgtaaaagaa
    17941 gtaagactta gaccaggtat tgaacaaaat gatttaaata cgaaagctaa taatgctata
    18001 aagttcctta aaaaagggga taaagtcaaa gttgaactta gatttagagg aagagaatta
    18061 ggacataaag acataggtaa agaagttatg cttaagtttt tagatataat aaaagagttt
    18121 ggagaaccaa caaaagcccc tgcatttgag ggtaataata tggttgtaat tatagatcca
    18181 aaaaaataga tgattaactg agaggaggaa tttgtcatgc ctaaaatgaa aactcataga
    18241 ggtgcagcaa agagattaaa gaaaacagga actggaaagt taaagagagc taaagctttt
    18301 aaaaaacata tattaactaa aaaatctgca aaaactaaaa tgaacttaag aaaatcaact
    18361 ttagtaagtg atggagatgc taagagaata gcacaattat taccatacta agatacagat
    18421 tgtaaataat aactatagaa gagggatata aaaggaggtc ataacgatgg caagagttaa
    18481 aaaagctatg aacgctcgta aaaagcataa gaaaatatta aaacttgcaa aaggattcag
    18541 aggatctaga agcaaattat atagaccagc aaatacattc gtaatgaaag cattaaaaaa
    18601 tgcttatata ggaagaaagt taaagaagag agacttcaga aaactttgga tacaaagaat
    18661 aaatgcagct gctagaatga atggaatatc ttattcaaga ttaatgaatg gattaaaatt
    18721 atctggtgtt gaagttaaca gaaaaatgtt atctgaaatg gctatacaag acccagaagg
    18781 atttgcaaaa ttagcagaag ttgctaaagc aaaattagct tagtattgaa tcatgagatg
    18841 taaagtcttt gagttatata aataattcaa ggactttttt attttttgaa aaatacttat
    18901 tagtgtcatg tacaaccaat tttataatga taaataaaat aagcaataca atagaatttt
    18961 atgagattta ttataagaat tgttacaatt atttatgata gctataaaaa gtcatagtaa
    19021 aaatgatatt gatgtatgtt gatatatgta taaagtttcc aatcaatctt gaattaatat
    19081 tatcaacatg ataatattat cttattaata atataaataa gggggacata atatgaatga
    19141 aaaactaatg gattgttttg taaacccaat taaatgcaag ttattgattg aaattttttc
    19201 aaataatcga actacagcta aacaattagg agaaaaattt caagatatat caaaggcaac
    19261 actttatcgt taccttaata aaatgttgaa agatgatatt ataaaagtgg ttgaagaaaa
    19321 taaaataagg ggaactgttg aaaaagtata tgctatgaag ttagatatag ctgatgatgt
    19381 gaacaaaatt atagatgaga actctggaga agaatatgct caactgttta ctcaatatat
    19441 gatgggattt ttaagagaat tttatgaata cgctgaaaga aaggaaaagg atatcctaaa
    19501 agatggttca ggattttctg tctgtccagt atatgctagt attgatgaga tagttgaagt
    19561 gagtaaaaaa attgctagta tactagaacc tctaagaaat aactcacatg aagaaggtag
    19621 aagaatgcat tctataggag tgataattac tccacctaaa aaggatgaag ggaaataaat
    19681 ctgatttaca tggggtagag ccaatttata cagggaatcg ttttatgatt tacatatgta
    19741 tccagagcaa agtatctctg cttggattgt acttcattgt accatttcat ccaatgactt
    19801 ggagtggtac aatgaagttt tttaattttt catattaatg ctaaagaaat gaactttaga
    19861 ccacatatgc aattaggttg cattatacaa ttagaccaca tatacaaaat aaaaatcagt
    19921 atttttatag cagataaatt gactgtcatt cttcttaaaa atcatctatt agttattgtt
    19981 agcatattga aagaaattat agtaatctat tgaataaatg aatattgcct ataatcatat
    20041 aaactgctat agtttaaaat aaagcaggac gttaaatttt atagtaatct aaaaatttaa
    20101 gagggggatt ttatgaaaat aaaatttgtt attatgccat tggtgatatt tttaatggta
    20161 ttatttgtgg ggtgtcaaag tacaaaagat acagaagtta aagcaaaaga tacagaaatt
    20221 aaaatattag ggacaggtga tttacattca attttgacag actctatggt ttcgtatgta
    20281 aatgaggaaa gggaaaagaa taaaaatctg cttatggtag atgcgggaga tttctatggt
    20341 acacaaagta gagaaatgtg ggaatggtct tctggaaaaa agttaattaa tattaaaaga
    20401 aatggtagag cagagtatga tgacataatt aagagtagta aagatgaagt acctatagtt
    20461 aaagatatgg caaagttgaa atatgatgct gtagttttgg gagataatga atttgtttca
    20521 aatgataagc aaagtcttga taaactggtt tctgatttta aaaataataa tatgccattg
    20581 gtatcagcaa atatatatga acaatctgga gaaaattatg tacaaccata tgtaatgaaa
    20641 aatattaaga cagatgaggg caatgtaaaa gttggaatac taggtcttac tataaaagaa
    20701 gtaggggaaa cccttggtct tgaggatttt gaaaaggata aaaaagctag agagcttgga
    20761 gaacaagctg attacaaagg aaaattatat gcaaatgatt tagtagaaga tgcaaaaaaa
    20821 tggataaaag ttatggaaaa agaaagtcca gacataatag ttgcagttgt acactcaggg
    20881 gaagaaccta aaacagatag aaatccagga aatagaataa aagagttagc aactacagtg
    20941 gatggaatag atgcaattgt agctggtcat actcatgaaa aaatagatga acacaaatat
    21001 aaaaataaat ctggagatga agtaattgtt acacaaccag gttggcatgg agatagagta
    21061 tcagagatta atttcaaact aaataaagag aatggaaagt gggacataaa ggaaaagtct
    21121 agtaaattaa aaataattga taaagaagta agtatagttg ctactgcaga tttacattca
    21181 catttctctg ataaattaac agttgacctt gttaatgaac gaagtaaccc aattgaacct
    21241 gttgtagtag atgcaggcga cttcttagac cctcaaacag atgaaatgac tcaatggtat
    21301 aaagagtgga aaagtataag agataataat agtgataaaa cgattagtag atgcccaatg
    21361 gtcatggata tgaatcaggg aatgtatgat gcagtagtac ttggaaatca tgaatttgta
    21421 tctgaaaaag atgaattttg gtcagatgaa cgccttttag atgctgttat agaggatttt
    21481 gaacaggtgg caatttctgt gttatcggca aatatatata ggcaatctgg taaaaattat
    21541 gttaaaccat atattataaa agatgtggag acgagtgaag gtaatgtaaa agtaggaata
    21601 ttgggactta ctataaaaga agtaggtaaa ggtcttaaaa atgtaaactt acaagagcaa
    21661 tttcaatata aggataaatt gtatgcaaat gatttagtgg aagatgcaaa gaaatgggta
    21721 aaagttatga aaaagaaaaa accagatata atagttgcag ttgtacattc aggagaagaa
    21781 ccaaaaaatc caaaacatcc aggaaataga ataaaagaac tagcaactac agtagatggt
    21841 attgatgcaa ttgttgctag tcatactcat gaaaaaatag atgagcatga atataaaaat
    21901 aagtctggag aaaaagttat agtgacacaa ccaggtgagc atggtaaata ttattctaaa
    21961 attaccttta aagtaaataa agaaaagggt tcgtggacta ttaatgataa attcagtaaa
    22021 acaattgagg ttgaataact ttataaaatt tatagataaa gtaaattatc tgatattggc
    22081 atgatatata agataaagtt tctaatatta gtataatgca taatgtgaaa tattttaata
    22141 ataagagaat aaacaagaca aataaaaata ttttaacatg taagatatag tatgaactat
    22201 tatactaatg cttactttat aatttgaaca tgttagaata agtcttattt gaaaatttta
    22261 gtgtactaga gtaagtatta ttttactagt aaaaaatatt gagaactata tgtagatttg
    22321 cttaaaaagg aatttacata tagttctttt cgtataataa atagtacaat cttatatggt
    22381 aaagataaag tgtgatatta aattttatta tcatccaaaa tctaaggagg agtctcttat
    22441 gaaaataaaa tttattatag tagcatcaat aatattttta atagtattac ttgtggggtg
    22501 tcaaagtact aaagacacag aaatcaatgt actcgccaca agtgatttac attcaattat
    22561 tccagataat ttaatttcct atgtagaaga ggagagaaaa tatgatgaga atctacttat
    22621 ggtagatgca ggagattttt ttgacatgca aagtggtgag atgaataagt ggtttactgg
    22681 tcaaaaatta gtaagattta aagatgggat accagagtac aaaaaaatag ctgaaccacg
    22741 tgaaggagaa gttccattgg ctaaaaaaat ggcaaagctg aagtatgatg cagttacatt
    22801 tgggaatcat gaatttgtat ctaatgacaa gcagagcctt gataaattgg tttctgattt
    22861 taaaaataac aatataccat tagtatcagc aaacgtatat gaacaatctg gagaaaacta
    22921 cgtaaagcca tatgtaatga aaaatataaa tactgacaag ggaactgtaa aggttggtat
    22981 acttggtctg actataaaag aagtagggga acgttcaagg tttaaaagtt ttgaacaaga
    23041 taaaaaggct agagaacttg gggaacaacc tcaatacaaa gggaaattat atgcaaatga
    23101 tttagtagaa gatgcaaata agtgggtaaa tgttatggaa aaaaaagaaa agccagatat
    23161 aatagttgcc attgtacatt caggtgaaga gccaaaaaat cctaaaaatc caggaaatag
    23221 aataaaagaa ctagcaacaa ctgtagaggg aatagatgct attgtagcag gtcatacaca
    23281 tgaaaaaata gaacagcata catataaaaa taattctggt gaagaagtaa tagtaacaca
    23341 gcctggagct catggtaata gtatatcaaa gattaatttt aagttggata agaagaatga
    23401 taagtggttt ataaaagaca aatatagtga attaaaggtt tttggaaagg aagtaaatat
    23461 aattacaact tctggtctga atccaaaatt ttcagatgaa atggcagtta atttgtttaa
    23521 tgaaagagat agagatgttc aacctatctt tatagatact ggagattttt taaatactaa
    23581 tacaaaagaa atgactaagt gggctaaaga gtggcaatac aatgaaaata aaggcatcta
    23641 taaaaaaata aatgaatggc caattgtata tcttggttat gatgctgtag tacttggtag
    23701 ccatgaattt acagaggata aagagacttt tgaggaaaaa aaatctacat tggattatat
    23761 tatagaaaac tttgaagaaa tgaagatacc agtattatca gctaatatat atgatgaatc
    23821 tggagaaaat tatgtaaaac cttatataat tcataatgta gaaactaggg aaggaaatat
    23881 aaaggttgga gtattaggtc ttactactac tgataaggat gaagaagact taagtaactc
    23941 aagcttaagc aaagactcaa agacttcgaa aattcctgaa gaaacaggaa agttatatac
    24001 taataatcta gtaaaagatg caagcgaatg ggtaaaagtt atgcaaaagg aaagtcctga
    24061 tgtgattgtt gtagttgtac attcagataa taatagtact ctaaaaaatc caagtagtga
    24121 agtcaaaaaa cttgcaatgt cagtagatgg aatagatgct attgttgtag gtcatacgaa
    24181 aaataaggta gaagaacata tttacaaaaa taagtctggg gatgaggtta tagtaacaca
    24241 atctggagaa gattgttatt caaaaataag tcttgaacta agaaaagaaa atggcaagtg
    24301 gaatatcata aacaaatata gtaaagcaat aaaaatgaag tgaatttatt gatatataaa
    24361 ccattattaa cactgatttt tataatataa taaatactg
//
LOCUS       DAMJVK010000057        24187 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.058, whole genome shotgun sequence.
ACCESSION   DAMJVK010000057 DAMJVK010000000
VERSION     DAMJVK010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24187
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.058"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            81..2258
                     /locus_tag="PC414_002349"
     CDS             81..2258
                     /locus_tag="PC414_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF2464739.1"
                     /translation="MYNKYNNLYLLLGKENVNMDSYKQLKAKIPCKETGIEIKRTLCD
                     ICSPGVHCGIDAYVKDGKVIKIEGTKEHPMNKGLLCTKGLCNREYIYREDRIKTPLKR
                     IGKRGEGKFKPISWDEAYREIAEKLNRVKSEYGAKHVAFFTGYGKWYRPFLQRFASSF
                     GSPNYGTESSSCSTSAQMAGNVATGRNFTMQDMENAKTYLGWGFNPYYSKFLNSVNLI
                     KLKEKGLKFIIVDPRITPTTQKLADLHLRIKPGTDGALALGMANLFIQKDLIDREYIE
                     KHVHGFNEYKEYVKLFDLDTVSRITGVSKDNIVKAVEMLSQGPFCINESAAPIVHHQN
                     GFQNYRAMMSLSAITGNYDRIGGNIPKQDYFAGKIGGFQTLEKEFVKEVLPKNINERI
                     GGERFPLWNEMVTQFQAMDLPRQIEEKTPYEIRALFCHGINARMFPNSGRLFKALNSV
                     DFFVNIDLFMTDTSKYADILLPACTSFEREECKSYPGGYITYTKKVIDKLYDSKSDVE
                     ILSDLANAMNIDDDLLKAGYEASVRHMFKNTCVDVDKLKESDLPLKCKDFKPYIVGSY
                     TREGYDTSTSRFELKSTIIEKYKDFGLDALPTYRGFNDNIDDEYTHLLTSGARISNAL
                     HSRLHDIKLSRILKNEANCDISSLDAKELGVISGDYIKITTKIGSLDVKVNVTDRVLY
                     KNIHMYHGYREADINSIICDNFDPYSGFPAYRSCKCKIEKMNR"
     gene            2272..2694
                     /locus_tag="PC414_002350"
     CDS             2272..2694
                     /locus_tag="PC414_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2464740.1"
                     /translation="MKSLFKFNKELCTACGACLLACIDQNDNNIDNGELFRHVFTIEE
                     GENIEYFSLACIHCNEPKCLGVCPKNCFKKEEGFIVLDNQNCIGCKLCEKACEYGALI
                     YGNDGKANKCNGCIERYKCGMDSPCVMVCPTGALKFNK"
     gene            2868..3443
                     /locus_tag="PC414_002351"
     CDS             2868..3443
                     /locus_tag="PC414_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2464741.1"
                     /translation="MVKVLAFMGSPRKKGNVDTILDEIIKGVNDNNVEVKKYYLNDMN
                     IKGCQGCLYCRKVPTCAFKDDMIDIYEDIKSAEYIIIGSPVYICQVSAQTKLLLDRLY
                     PLTEINKSKHIPRFGQKKLIMVYTQAAPISFLFKKYFKYTAKHLKGMGLNHYKTIVVT
                     KAFERNSTKKNFKVLKKAYKLGMKIAIDING"
     gene            complement(3568..4284)
                     /locus_tag="PC414_002352"
     CDS             complement(3568..4284)
                     /locus_tag="PC414_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDF2464742.1"
                     /translation="MQQGSDYLKKFLEKHIDLLKKIPLFEGIKSDELEEMLECLGVVD
                     KTYPKDSIIFSVGSEVTSIGIMLKGSAHIIKEDIEGNRNIVAELLPGDLFGEVFACTR
                     LHKSNVTVTTTSSCEVLFIKFKSVTGICSSACVFHNRLVENMLQLIAEKNILLHNKIE
                     LLSRKTTREKLLMYFSKQIEQTGSHQFTIPFSRNEMADFLCVDRSSMSRELGKMRDEG
                     LLSFNKNKFEIFYFQNKLDI"
     gene            4449..5174
                     /locus_tag="PC414_002353"
     CDS             4449..5174
                     /locus_tag="PC414_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2464743.1"
                     /translation="MMRKIIKIDEEKCNGCGLCVEACHEEAIGMVNGKAKLLRDDYCD
                     GLGDCLPTCPTNAISFEYREAAEYDEAAVKANMEAKKAQKKTLACGCPGSQSKSINRE
                     VSNSTSISNDIVEIKGSQLNQWPVQIKLVPTNASYLKNASFLIAADCTAYAYGNFHNK
                     FMKNKVTLIGCPKLDEVDYAEKLTEILKENDIKNIVVTRMEVPCCGGIVNAVKTALQN
                     SGKMIPWQIVTISVDGKIVDGEI"
     gene            5234..6826
                     /gene="hcp"
                     /locus_tag="PC414_002354"
                     /gene_synonym="priS"
     CDS             5234..6826
                     /gene="hcp"
                     /locus_tag="PC414_002354"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HDF2464744.1"
                     /translation="MEKMFCFQCEQTAGGKGCTINGVCGKKGGTANLQDELTGVLIGL
                     SRATIGNKNRPTSETDKIMIEGLFTTITNVSFDDEAIKRQIEKTEVEKSKLVPRCSDC
                     STTCNRNDNYDMKDLWNDNEDIRSLKSLILFGLRGMAAYAYHAMVLGYTDKDVNEFFY
                     EGLIAVGGDLSIDELLGLVMKTGEINLKCMELLDRANTETYGTPEPTQVSMKIEKGPF
                     IVVTGHDLYDLHKLLEQTKDKGINIYTHGELLPAHAYPKLKEYKHLKGNFGTAWQNQQ
                     KEFDNIPAPILFTTNCLMPVKDSYRDRVFTTEVVAYPGMVHIDEDKDFSSVIEKALEL
                     GGYREDVHMTGINGGDTLTTGFARGTVSSVQDKVVDAVKSGAIRHFLLVGGCDGAKPG
                     RNYYTEFVKQAPKDTVILTLACGKYRFNDLDLGDIGGLPRLMDMGQCNDAYSAIKVAV
                     SLANAFECGVNDLPLSMVLSWYEQKAVCILLTLLHLGIKNIYIGPSLPAFLSKNVLDI
                     LVDKFALTPISTPEEDMKKILG"
     gene            7198..8103
                     /locus_tag="PC414_002355"
     CDS             7198..8103
                     /locus_tag="PC414_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDF2464745.1"
                     /translation="MKNSNFNILAFDGGGLKGALSISILERIVKEYPNLLNNINMFGG
                     TSTGSLIALGLAYGVSPKEIKELYSIENSKYIFNKSYAEILRPKYENKNLKEVLLSIF
                     PEELELKDLNKLVMIPSFYIGNEENAWKPVFYNNMPNSFTKTSKVVDVAMASSAAPVF
                     FPTYNRHVDGGIIATDPSLACIIHAIDSGFKLKNTRLFSIGTGYVYNSIKADTTEWGA
                     IDWIINKEPDLPIISITLEGNSQMSQLFSQKLLGDNYYRLNPKMEKDVAMDDCDALEY
                     LMSLGENCDIKDAFGWIKKKSFYRN"
     gene            8227..9180
                     /gene="msrA"
                     /locus_tag="PC414_002356"
     CDS             8227..9180
                     /gene="msrA"
                     /locus_tag="PC414_002356"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDF2464746.1"
                     /translation="MIKLATFSGGCFWCMVKPFDKYDGVIKVVSGYTGGHVENPTYQE
                     VCSGTTGHLEAIQITYDDDIISYDELLNIFWKQINPTDDGGQFGDRGTQYKTAIFYHD
                     EEQKNIALESREHLENSKIFDSKIVTDIIPATKFYEAEENHQDYYKKRPLNYNMYYKG
                     SGRYNFVKSHWDGNNINREELKKTLTPIQFEVTQNDMTEVPFENEYYDNNKEGIYVDI
                     VSGEVLFSSKDKFDSGCGWPSFSKPVIDKSIIERSDFSQGMYRVEVRSAKGNSHLGHV
                     FDDGPKELGGLRYCINSASLKFIPRENMEALGYGEYLKLFE"
     gene            9333..10463
                     /locus_tag="PC414_002357"
     CDS             9333..10463
                     /locus_tag="PC414_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2464747.1"
                     /translation="MNLKDFNGKRLKSARIFRAKTIEQLSKETKINKKDLKAFEENKY
                     VPNIENTLKLSNILNFPKEYFYKNENINIVVEDSHFNPQSKLPRVEEISYREKIIIIH
                     KIYSFMENYIKFPKENLPNRKVMSDININDIESLAYKTREFWDLNSTPIVNMVSLLES
                     KGIIISGMNVDRKGSTIFTQKQRISKESKYLISLGNDKKSASIRNYTLACELGYIISS
                     ELRIPSKQFSEDEYACAFLLPKESFLKDLTHPEDLDYYVELKKKWIVPISAMIFRAYN
                     LEKINYKKYNYLMNEMDKKGWLIEEPLDKMKGSSPTYLKRAVELLIENKIMSVNSIVS
                     SLEEFGINLYPEDLELLMGLKKGLLSQEVNKKSKVIKFDGKK"
     gene            complement(10580..11548)
                     /locus_tag="PC414_002358"
     CDS             complement(10580..11548)
                     /locus_tag="PC414_002358"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDF2464748.1"
                     /translation="MAIKPRKISIVGSGHVGSHCGFSLITQGVCDELFMIDIDESKSK
                     AQALDLADAVSYLPHKVHIEKGTFSDCKDSDIVVISVADSSEGPLRRQNTTRLDLLRP
                     TIGMIKSIVKPIVDSGFDGIFVVISNPVDVVTNYIWEKSGFPKNKVIGTGTALDSTRL
                     RRILSEETGIAQQSIQAYSMGEHGDSQMVPWSHVSIGGKPILDMIKDNPSTYSNLDLP
                     SIVEKNKKTGISIINGKDCTEFGIGTALVEIVKAILHNEKKVLPVSTLLEGQYNERNV
                     FAGVPCVIGKDGIEEIIEINMTEYEQNEFNKSCSVLREYIALSKTL"
     gene            11883..12071
                     /locus_tag="PC414_002359"
     CDS             11883..12071
                     /locus_tag="PC414_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464749.1"
                     /translation="MNYHTKEELVEALRVVSSSIINCGKGQKKFSEETSHHTRFKNII
                     EVMYISKSLIMDEISKRD"
     gene            12331..13530
                     /locus_tag="PC414_002360"
     CDS             12331..13530
                     /locus_tag="PC414_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein AlaX-L"
                     /protein_id="HDF2464750.1"
                     /translation="MEKLYYTNQYIRDFTAEIIEIKEVENKYHVLLDKTAFFPGGGGQ
                     FGDLGKIENDDVINVYEDGKKIYHVLDKKPIKIHKVKCSIDWDRREDGMHQHFGQHVL
                     SGCFFKTFNANTTGFHLGNEYSTVDIEGNLEEDKIREIELFANEIIRQNIQVETLLPT
                     KKELKKIWLRRDLPNTDEEIRVIKIGDLDTNACCGVHPKSTGELRMIKIKKWEKHKSS
                     TRIEFLAGKRAVDEVLKRDIYLTKICRYLSCSEEDAINGIVNLNSKVEEALIKKRRLE
                     EVVSTYQVKEMIEKANKVGNISVIKKIYDDEDLKYVNKIANKIAEYENSIALLAVKFN
                     DKINLVFACSKNLKDINMGVLLKDAINLIDGKGGGSKVLAQGGGKNNGNLEATLDYAF
                     MKIEKTL"
     gene            13582..14085
                     /locus_tag="PC414_002361"
     CDS             13582..14085
                     /locus_tag="PC414_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2464751.1"
                     /translation="MEQQKDYIELYENEELIEIREARMDDLDTIAKFNYNLAKETEGK
                     ELDMDVLTKGVKALLLDERKGKYHVYTVFDKVVAQIMYTYEWSDWRNGNFLWIQSVYV
                     DKEYRRKGIFNYLFNYIKNICDKDENIVGMRLYVEKENINAKATYESLNMYECDYNMY
                     EYEVVHS"
     gene            14192..14509
                     /locus_tag="PC414_002362"
     CDS             14192..14509
                     /locus_tag="PC414_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464752.1"
                     /translation="MGVLKGMFYVFLFLDLVSIFCFFFNKGKIASNKIVFNAIGVLTF
                     VLCFMLFSYYPNNNLIGKFIASLFFIFGVAGVMLKEKNFLYARLLLTVVIVFSTLRLF
                     VIQ"
     gene            14667..15626
                     /locus_tag="PC414_002363"
     CDS             14667..15626
                     /locus_tag="PC414_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDF2464753.1"
                     /translation="MIESKKSEKLLLSIIYIIFLVYILMIIRIILFRDVPIYAIFKGT
                     FRNVNLIPFYTIYQFIVDSNLDFMKATVNIIENIGIFIPMGIFLPIVCKNLNKKTIII
                     VIILVSLAFELTQYIFALGSSDIDDVILNSLGGIIGINIYINMNKLFPNDINRFKAII
                     GTSLVLGVIGLGVISKNYHNLLTFKFRPDRKISKILIEENREIIKDINKDTVDIVGTF
                     ESFKKGIITIKAGSNNKVKRPENLIDSDGNIRIYLNENTKLVSYIITKESKMDIVKYE
                     EFDVKNLDLLRRYDTINVWIDKENQPKDKHGIMASKLLIGLYE"
     gene            15741..16526
                     /locus_tag="PC414_002364"
     CDS             15741..16526
                     /locus_tag="PC414_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464754.1"
                     /translation="MEIIKYILYFFIYSFLGWTVESIGCSIASKRIINRGFLNGPICP
                     VYGFGAVIVISLLGRFNNVVIVFLLGMILTTILEYFTGFILETLFHAKWWDYSDRKFN
                     IKGRVCLKNAIYFGVMSVLIIRFIHPFIKYFVSIIPYRILISMAIITTLWTILDLIVT
                     IITLKKLDIKLNLLDDIITDLNDINVKLDKFDRGEIQALFKTINNDELEVREKINKIN
                     SKLDRIESNIILQKRVIKAFPHIKHKKQQEQLEHFKKIINEKR"
     gene            16818..18137
                     /locus_tag="PC414_002365"
     CDS             16818..18137
                     /locus_tag="PC414_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF2464755.1"
                     /translation="MSEITSQMISTEEKKYVAKTQKIPYYPVAFKSGDGAMLYDYEGN
                     EYVDFLASAGSANVGHGNKEISQAVKEQMDDITQYTLAYFHSDPPVKLAEKLVEIAPG
                     DNDKKVLYSATGSACIDAAIKLARGYTGRTKIISMCESYHGSTYGAISISALSTNMRR
                     KMGPLLPEVYHFHYPDKNRTAKECLDEIEYAFAHYLPAEEVAAIFIEPIAGDAGIIVP
                     PVEWVQGLSKICKENGILLVSDEIQQGMGRTGKWFGIENFGVEADLIVLGKSVGGGLP
                     LGAVVGRTEIMQSLDAPAHLFTLAGNTTVCVAALKSIEIIEKENLLQKSIEMGDYIKA
                     GFEKLKEKYDIIGEIRGIGLSIGVDIVKGKGSNEKHPDATAKICYRCIQTGLIMIFLG
                     QSTLRVQPPLVITKEQVDKAMNIIDSAIDDYLNGRIGDEVYEVTQGW"
     gene            complement(18212..18640)
                     /locus_tag="PC414_002366"
     CDS             complement(18212..18640)
                     /locus_tag="PC414_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464756.1"
                     /translation="MKDNNNTNKTIQFGEKKASLENNIQKNKNIKLDNTHKVIPYPKN
                     RKKDDKKKGNKKKNTNTSKVNKNNYGHSFFNNKYKYKRKPYVIALYTVILILLAILII
                     TKLTDSTNKGNYSRNTVSDTHKNDIRNIVFNESNNFLICG"
     gene            complement(18731..20107)
                     /gene="hydG"
                     /locus_tag="PC414_002367"
     CDS             complement(18731..20107)
                     /gene="hydG"
                     /locus_tag="PC414_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HDF2464757.1"
                     /translation="MFINHELINSLLEDAKNSTSDDIEKVLDKADRREKLSYKDIATL
                     LEVEDKKQLDRLFSIAGQIKNEIYGNRVVLFAPLYVSNYCVNECVYCGFSKCNKFKRK
                     KLTMEEIKEEVKILEKMGHKRLALEAGEDPKNCDINYILDCLDAIYSTYNENGNIRRV
                     NVNIAATSVDEYKLLKEKGIGTYILFQETYHKPTFIKMHGQSIKNDYYYHLTAFDRAM
                     EAGVDDVGAGVLFGLSDPKFEVLGLMMHNEHLEEKFGVGFHTISFPRLKKAEGMSLED
                     FPHLVSDDMFKKIVAITRLAVPFTGIIMSTRETAEMRNELLKYGVSQISAGSLTGVGG
                     YKAYEDGDNTEQFEVGDHRSPVEVLKELITDGYIPSYCTACYRKGRTGDRFMSLAKSR
                     QIHNVCTPNALTTLNEFLIDYGDEELKIMGKKLIAEEIGKIEREDIRNIVSNNMTALE
                     RGERDLYL"
     gene            complement(20108..21193)
                     /gene="hydE"
                     /locus_tag="PC414_002368"
     CDS             complement(20108..21193)
                     /gene="hydE"
                     /locus_tag="PC414_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HDF2464758.1"
                     /translation="MTKNKIKYFIDKLYSINSLSFDELLYLIENIDNEEYNFEQNLRN
                     YLYKQASDMRNKHYGNRVYLRGLIELTNYCKNNCYYCGIQSSNKNIIRYRLSLDEILE
                     CCDIGYKIGYRTFVLQGGEDAYFTDDKICEIVSSIKNKYEDCAITLSLGEKSYDSYKK
                     YFLCGADRYLLRHETATDSHYKKLHPPNIELRTRKECLKNLKEIGYQVGAGFMVESPF
                     QNNEDLVRDLLYLKELNPHMVGIGPFIPHHDTIFKDYKHGDLEKTLLMLSITRLLLPK
                     VLLPATTALASINPSGRNAGLLAGCNVIMPNLSPQEFRKQYSLYDNKAFTGQEACEYH
                     QSLEENIKSLGLEVDYSRGDNIEWRRL"
     gene            complement(21229..22437)
                     /gene="hydF"
                     /locus_tag="PC414_002369"
     CDS             complement(21229..22437)
                     /gene="hydF"
                     /locus_tag="PC414_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HDF2464759.1"
                     /translation="MSLNSTPQSVRVHIGLFGKRNAGKSSIINAITNQSAAIVSDIAG
                     TTTDPVFRPMEILPIGPCVLIDTAGLDDVGELGELRIGKSLDVLEKTDIALLVVDCQI
                     GISQEDLSLIEKFNDKNIPHILILNKIDTIKNQSEILNLTKNKVKCPVVSVSSTDKIG
                     IENLKNEIIKVLPKDSTEFKLVSDLIEPNDLVVLVVPIDKAAPKGRLILPQQQVIRDI
                     LDNGAISIVTKEDSLKETLSNLGKKPKLVITDSQVFPQVDKDTQKDIPLTSFSILFAR
                     QKGDLKELINGAYALENLKDGDKILMAEGCTHHRQTDDIGTVKIPNMIRKKTGKNITF
                     EFSSGVSFTEDINKYALVVHCGACMMNRAGMLSRIEKAKSFNVPIVNYGVLIAYVKGI
                     LERSLELFNY"
     gene            complement(22452..22703)
                     /locus_tag="PC414_002370"
     CDS             complement(22452..22703)
                     /locus_tag="PC414_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HDF2464760.1"
                     /translation="MKKVAVISAILEEPKECQFKFNEVVSGFKGIIKGRMGIPCEEEG
                     VSIVCIAVVGEMDTINSLTGKLGNIPHVSVKTSISKKEV"
     gene            complement(23084..24124)
                     /locus_tag="PC414_002371"
     CDS             complement(23084..24124)
                     /locus_tag="PC414_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HDF2464761.1"
                     /translation="MNLNLTNTINLMKKYLDIPSPAGYTENAVLEIKKDFENLSLDTI
                     LTKKGALIATLPGEDDSNQITISAHMDTLGAIVKEISSDGTLKYHKVGGGCWAAIEGE
                     NCTVITRKGKKIRGSVVFKYASTHIYGQTKAATERNEETMIIRLDEKVFTKQDVLDLG
                     ISVGDFVCLDHRFEATESGFIKSRYIDNKSAVAMVLEICRYFKENNLTPKHTTNFYIS
                     NYEEIGHGVSKCLPEKTKEFVAIDIAPTGPSQTSSEHGVTIVAKDNLSLYDFNLRNKL
                     VDVAEDNNLDYAVDVFTFYGSDASEAIRWGEDVQIACVGPGTNNSHHYERTHIEAIEN
                     TLKLLINYMLKE"
BASE COUNT         9168 a         2855 c         3900 g         8264 t
ORIGIN      
        1 aaatataaaa tcttattatt aaaaatattc ttagaaatta cactgtataa aatataaagt
       61 atgatatttg aataaaaaga atgtataata agtataataa tttgtattta ttattgggaa
      121 aggaaaatgt taatatggat agttataaac aacttaaagc taaaataccc tgtaaggaaa
      181 caggtataga aataaagagg actttatgtg atatttgttc gccaggagta cattgtggaa
      241 ttgatgcata tgttaaagat ggtaaagtaa taaaaataga aggtacaaaa gaacatccaa
      301 tgaataaagg gttgttatgt accaaaggtc tttgcaatag agaatatatc tatagagaag
      361 atagaataaa aacacctcta aaaagaattg gaaaaagagg tgaaggaaaa tttaaaccaa
      421 tatcatggga tgaagcttat agagaaatag cagaaaaatt aaatagagta aaatctgagt
      481 atggagcaaa gcatgtagct ttttttacgg gttatggaaa gtggtataga ccttttttac
      541 aaaggtttgc atcttcattt ggttcaccta attatggtac agaatctagt tcatgttcaa
      601 catcagcaca gatggctgga aatgttgcta ctggaagaaa ctttacaatg caagatatgg
      661 agaatgcaaa aacttattta gggtggggat ttaatccata ctactctaaa tttttaaata
      721 gtgtgaatct aataaaatta aaagaaaaag gtctaaaatt tataattgta gaccctagaa
      781 taacaccgac aactcaaaaa ctagcagatt tacatttaag aattaaacct ggaactgatg
      841 gagctttagc tcttggaatg gcgaatctat ttatacaaaa ggatttaata gatagagaat
      901 acatagaaaa gcatgtacat ggatttaatg aatataaaga atatgtaaaa ttatttgatt
      961 tagatactgt aagtagaatt acaggagttt ctaaagataa tatagtaaaa gctgttgaga
     1021 tgttgtcaca aggtccattt tgtataaatg aaagtgcagc tccaatagtt catcatcaaa
     1081 atggatttca aaattataga gcaatgatgt ctttatccgc tataacaggt aattatgata
     1141 gaataggtgg aaacatacca aaacaagatt actttgctgg taagataggt ggattccaaa
     1201 cactagaaaa agagtttgta aaagaagttc tacctaaaaa tataaatgaa agaattggtg
     1261 gagaaagatt tcctctatgg aatgaaatgg tgactcaatt tcaagctatg gatttaccta
     1321 ggcaaataga agaaaagaca ccatatgaaa taagagcttt gttttgtcat gggataaatg
     1381 ctagaatgtt tcctaattca gggagacttt ttaaagcgtt gaatagtgta gatttttttg
     1441 tgaatataga tttatttatg acagatacat caaaatatgc agatatactt ttacctgcat
     1501 gtacatcatt tgagagagaa gaatgtaaga gttatccagg agggtatatt acatatacca
     1561 aaaaagttat tgataaatta tacgattcaa aatcagatgt agaaatactt tcagatttag
     1621 caaatgctat gaatatagat gatgatttat taaaagcagg ttatgaagca agtgtaagac
     1681 atatgtttaa aaatacatgt gtagatgtag ataaacttaa agaaagtgat ttaccattaa
     1741 aatgtaaaga ttttaagcca tatatagtag gttcatatac aagagaaggg tatgatacta
     1801 gcacttctag gtttgaatta aaatctacaa ttattgaaaa atataaagat tttgggctgg
     1861 atgcactacc aacatataga ggatttaatg ataatataga tgatgaatat actcatttat
     1921 tgacatcagg agcaagaatt tcaaatgcac tacattcaag acttcatgac atcaaacttt
     1981 caagaatatt aaaaaatgaa gctaattgtg atatttctag cttagatgca aaagaattgg
     2041 gtgttatcag tggagattat ataaaaataa ctactaagat aggaagtttg gatgtaaaag
     2101 tcaatgttac tgatagagtc ttatataaga atatacatat gtatcatgga tatagagaag
     2161 ctgatataaa tagtattatt tgtgataatt ttgaccctta tagtggattc cctgcgtata
     2221 gaagttgtaa gtgtaagatt gaaaaaatga atagataaat ggagagatat aatgaaaagt
     2281 ttatttaaat ttaataaaga attatgtaca gcatgtggag catgtttgct tgcatgtata
     2341 gaccaaaatg ataataatat agacaatggt gaacttttta gacatgtatt tacaatagag
     2401 gaaggtgaaa atatagaata tttttcatta gcatgtatac attgtaatga gccaaaatgt
     2461 ttaggtgtat gtcctaaaaa ttgttttaaa aaagaagaag gattcattgt attagacaat
     2521 caaaactgta tagggtgtaa actttgtgaa aaagcttgtg aatatggagc cttaatttat
     2581 ggaaatgatg gtaaggcaaa taagtgtaat ggttgtattg agagatataa gtgtggtatg
     2641 gattcacctt gtgtaatggt atgccctaca ggagcattaa aatttaataa ataattgtat
     2701 taaattgcta tactactttt aaataattgt gttaagaatt attttttgat atacttttgt
     2761 aatataaaat gtaaataaaa caaatacaaa gtatataaaa tatattttta gaataaagtg
     2821 tgctttagat taaatttaga aaaataaatt agacaggaga acttaatatg gtaaaagtat
     2881 tagcctttat gggcagtcca agaaaaaaag ggaatgtaga cacaatactt gatgagataa
     2941 taaaaggtgt aaatgataat aatgttgaag ttaaaaaata ttatctaaat gatatgaata
     3001 ttaaaggatg tcaaggctgt ctatactgta gaaaagttcc tacttgtgca tttaaagacg
     3061 atatgattga catatatgaa gatataaaaa gtgctgaata tataataatt ggttcgccag
     3121 tatatatatg tcaggtatca gctcaaacaa agctactttt agatagattg tatcctttaa
     3181 cagagataaa taagtctaaa catataccta ggtttggtca aaaaaagtta atcatggtct
     3241 atactcaagc agctcctatt tcgtttttat ttaaaaaata ttttaagtat actgcaaaac
     3301 atttaaaggg catgggatta aatcactaca aaactatagt tgtaaccaaa gcatttgaaa
     3361 gaaattccac taaaaaaaat tttaaggtat tgaaaaaagc ctataaactt ggaatgaaaa
     3421 tagccattga tatcaatgga tagataaaag tttaatttat agtatagcta aaggagcatt
     3481 aattattaat actccttttt tgtataaagg tttctagaaa ataactaata aaaaacaata
     3541 ttatttgaat acagatatta tacaagccta gatatctaac ttgttttgaa aataaaagat
     3601 ttcaaattta tttttgttaa aagataacaa gccttcatct cgcatcttgc caagctctct
     3661 tgacatagaa cttctgtcaa cacataaaaa atcagccatt tcatttcttg aaaagggaat
     3721 tgtaaactgg tggctacctg tttgttctat ctgttttgaa aaatacataa gcaatttctc
     3781 gcgagttgtt ttacgagaaa gtaattctat tttattgtga agtaaaatgt ttttttcagc
     3841 tattaattgt agcatatttt caacaagcct attatgaaat acacatgcag atgaacaaat
     3901 accagttaca cttttaaact ttataaataa aacttcgcaa gaagatgttg tggttactgt
     3961 aacattactt ttatgtagtc tagtacatgc gaatacctct ccaaataaat ctccaggcaa
     4021 gagttcagct acaatattcc tattcccttc gatatcttcc tttattatat gagcacttcc
     4081 tttaagcata attccaatag aagtaacttc acttccaaca gagaatatta tactatcttt
     4141 tgggtaagtc ttatctacaa cacctaaaca ttccaacatt tcttcaagtt catcactttt
     4201 aattccttca aatagaggaa tttttttaag taagtctata tgcttttcta aaaatttttt
     4261 caaataatca cttccttgtt gcatatgcaa ccgataattt gttttcattg tactatacta
     4321 agtttataaa agcaagggga tataaaaatc ctactacaag aaatttgaaa taaagaaata
     4381 taggtattaa aaataaatat ataagaaatc aatactttaa attaaaaaaa ttttatgagg
     4441 tgatataaat gatgagaaaa ataattaaaa ttgatgagga aaagtgtaat ggttgtggac
     4501 tatgtgttga agcttgtcat gaagaagcta taggaatggt aaatggaaaa gcaaaacttt
     4561 taagagatga ctattgtgat gggctgggag attgtctgcc aacttgtcca acaaatgcga
     4621 tttcatttga gtatagagaa gcagcagaat atgatgaagc tgcagtaaaa gcaaatatgg
     4681 aagcaaagaa agcacaaaag aaaactttag catgtggatg tccaggttct cagtctaagt
     4741 caataaatag agaggtttca aatagtacgt ctataagcaa tgatatagta gagataaaag
     4801 gttctcaact taatcaatgg ccagtacaaa taaaattagt accaactaat gcatcatatt
     4861 taaagaatgc aagtttttta atagcagcag actgtacagc gtatgcatat ggaaattttc
     4921 ataataaatt tatgaaaaat aaggttacat taatcggatg ccctaaactt gatgaagttg
     4981 actatgctga aaaacttaca gagattttaa aagaaaatga tattaaaaat atagtagtaa
     5041 cacgtatgga agttccttgc tgtggtggta tagtaaatgc tgttaagaca gctctacaaa
     5101 atagtggaaa aatgatacca tggcaaatag ttactatatc tgtagatgga aaaattgtag
     5161 atggagaaat ttaattaagt gaattataga aaaatataat aaattttaat aaataaaaag
     5221 ggaggaataa aaaatggaaa aaatgttttg ttttcaatgt gaacaaacag caggaggtaa
     5281 aggttgtact ataaatggtg tctgtggtaa aaaggggggt acagctaact tacaagacga
     5341 acttacagga gtgttgattg gtctttcaag agcaactatt ggaaataaga atagaccaac
     5401 aagtgaaaca gataaaatta tgatagaggg gctattcact actataacga atgtaagttt
     5461 tgatgatgaa gctataaaaa gacaaattga aaaaactgag gtagaaaaaa gtaaacttgt
     5521 acctagatgt tcagattgtt caacaacttg taatagaaat gataactatg atatgaagga
     5581 tttatggaat gataatgagg acatacgttc attaaaatca ttaatcttat ttggattaag
     5641 aggtatggct gcctatgcat atcatgcaat ggtacttggt tatacagaca aagacgtaaa
     5701 tgaatttttc tatgaaggat taatagcagt tggaggagac ttgtctattg acgaactttt
     5761 aggtctagtt atgaaaactg gagaaataaa cttaaaatgt atggaattgc ttgatagagc
     5821 aaatactgaa acttatggaa caccagaacc gactcaggta tctatgaaaa tcgagaaagg
     5881 gccatttatt gtagttacag gacatgacct ttatgattta cacaaattat tagaacaaac
     5941 gaaagataag ggtataaata tatatactca tggtgagtta ttaccagcac atgcatatcc
     6001 aaaactaaaa gaatataaac atctaaaagg taattttggt acagcatggc aaaaccagca
     6061 aaaagaattt gataatattc ctgctccaat attatttaca acaaactgtt taatgcctgt
     6121 taaagatagt tatagagata gagtatttac tacagaagta gttgcttatc caggtatggt
     6181 tcatattgat gaagataaag atttttctag tgtaatagaa aaagctcttg aacttggagg
     6241 atatagagaa gatgttcata tgactggtat taatggagga gatacattaa ctactggttt
     6301 tgcaagaggt acagtttcat ctgtacaaga taaagtagta gatgctgtaa agagtggagc
     6361 tataagacac tttttattgg ttggaggttg tgatggtgca aaacctggaa gaaattatta
     6421 cactgaattt gtaaaacaag ctcctaaaga cactgttata ttgacacttg catgtggaaa
     6481 atatagattt aatgatttag accttgggga tataggtggc ttacctcgtt taatggatat
     6541 gggacaatgt aatgatgctt atagtgcaat taaagtggca gtttcattgg caaatgcttt
     6601 tgaatgtgga gtaaatgatt tgcctcttag catggtactg tcatggtatg agcagaaagc
     6661 tgtttgtata cttcttacac tacttcattt aggtatcaaa aatatctata tagggccttc
     6721 tttgccagca ttcttatcta agaatgtatt agatatattg gttgataaat ttgcattgac
     6781 acctatatct actcctgaag aagatatgaa gaagatttta ggataaataa acatatctat
     6841 acttaaagat atttatagtt aaaatatttt taagtatgcc acaattaaga taaggggtta
     6901 aacttaaaat ttgattaaaa aatcaatttt gagatgaccc tttatttatt ttttgtaata
     6961 attgaaaaat aaacctgctt tcagtatatt ctttttaaga atctttgtat taaatttata
     7021 atacttagat atataatatc ttatatattt tattttaagt attaaaggtt tgaatgtaac
     7081 ttaaatatta aaagaggaac attgtaatat gtaacttgca taaaatatat atactatagt
     7141 gaaattattt tgattaaaat ttaaaatata aggtgtttta tgtgggggta tatgtatatg
     7201 aaaaattcta attttaatat attggcattt gatggtggtg gattaaaagg ggcattgagt
     7261 atatccatac ttgagaggat agtaaaagag tatccaaatc tattaaacaa tataaatatg
     7321 tttggaggta catctacagg gagtctaatt gcactagggc ttgcgtatgg tgtaagccct
     7381 aaagagataa aagaacttta ctcaattgaa aattcaaagt atatttttaa taaaagttat
     7441 gcagaaatac tcagaccaaa gtatgaaaac aaaaacttaa aagaagtttt attaagtata
     7501 tttccagagg aactagaatt aaaagattta aataaattag taatgatacc aagtttttat
     7561 ataggaaatg aggaaaatgc atggaaacca gtgttttata acaatatgcc aaattctttt
     7621 acaaagacta gcaaagttgt agatgtagca atggcaagta gtgctgctcc ggtatttttt
     7681 ccaacatata atcgtcatgt tgatggagga ataatagcaa ctgacccgag tttagcgtgt
     7741 ataattcatg ccattgacag tggatttaaa cttaagaata caagactttt ttcaataggg
     7801 actggatatg tatataacag tataaaggca gatactaccg aatggggagc tattgactgg
     7861 ataattaata aagaacccga tttaccaatt atatctatta ccttagaagg aaattctcaa
     7921 atgagtcagt tattttctca aaaacttctt ggagataatt actatagatt aaatcctaag
     7981 atggaaaaag atgtagctat ggatgattgt gatgcattag aatatctaat gtctttagga
     8041 gaaaattgtg atataaaaga tgcttttgga tggataaaga aaaagagctt ttatagaaat
     8101 tagattcaat ttgcaattgt atttttacac atgatttcat tgagtaggta aaaataataa
     8161 aatgcagatt attaagttac ttcttaggga ataaataaag tataatttat ttaaggagtg
     8221 atgaaaatga taaaacttgc tactttttca ggtggatgtt tttggtgtat ggtaaaacct
     8281 tttgataagt atgatggagt tattaaagta gtttctggat atacaggtgg tcatgttgaa
     8341 aaccctacat atcaagaggt atgctcagga actactggtc atttagaagc aattcaaata
     8401 acatatgatg atgatataat aagttatgat gaacttctaa atatcttctg gaaacagatt
     8461 aatcctacag atgatggtgg acaatttggt gataggggaa ctcaatataa gactgctata
     8521 ttttatcatg atgaagaaca aaaaaatata gctttagagt caagagaaca tctggaaaat
     8581 agtaaaatat ttgatagtaa aatagtgact gatataattc cagcaactaa attctacgaa
     8641 gcagaagaaa atcatcagga ttattataag aaaagaccat taaactacaa tatgtattat
     8701 aaagggtctg gaagatataa ttttgtaaaa agccattggg atggaaacaa tataaatagg
     8761 gaagagttaa aaaagactct aactcctata caatttgaag tcactcaaaa tgatatgaca
     8821 gaagtacctt ttgaaaatga atattatgac aataacaaag agggaattta tgttgatatt
     8881 gtaagtggag aagtgttatt ttcttcaaaa gataaatttg attctggatg tgggtggcca
     8941 agtttttcaa agccagttat agataaatct atcattgaga gaagtgattt ctcacaaggt
     9001 atgtacagag ttgaagtaag aagtgctaaa ggaaattctc atttaggtca tgtatttgat
     9061 gatggtccaa aagaacttgg aggtttaaga tattgtataa attctgcttc tcttaaattt
     9121 ataccaagag aaaatatgga agctttagga tatggagaat atttaaaact ttttgagtaa
     9181 ttaggaatat ttattataaa cttatctaaa aggtgaaaat ataaaaaaat attttcacct
     9241 tttgtttttt tgtcaataat atgataaatt attaattgta atcttattta taccaataga
     9301 ttaagaattt aattttgaaa gagagaaaac aaatgaattt aaaagacttt aatggaaaga
     9361 gattaaaatc agctagaatt tttagagcaa agacgataga acagttgtct aaagaaacta
     9421 aaataaataa aaaagattta aaagcttttg aggaaaataa gtatgtacca aatatagaaa
     9481 atacattaaa attatctaat atattaaatt ttccaaaaga gtatttttat aaaaatgaaa
     9541 atataaatat cgttgtagag gatagccact ttaaccctca atctaaacta cctagagtag
     9601 aagagatttc ttatagagaa aaaataatta taatacataa aatatattca tttatggaga
     9661 attatattaa gtttccaaaa gaaaacttgc caaatagaaa agtaatgtca gatattaata
     9721 taaatgatat agaatctctt gcatataaga ctagagagtt ttgggattta aatagtactc
     9781 caatagtaaa tatggtaagt ttgttagagt ctaaagggat tataatatca ggtatgaatg
     9841 tagatagaaa agggtcaact atctttactc aaaaacagag aattagtaaa gaatctaaat
     9901 atctaatatc tctgggtaat gacaaaaagt cagcatcaat tagaaattat actttggcat
     9961 gtgagcttgg atatataata tcaagtgaat taagaatacc aagtaagcaa ttttcagaag
    10021 atgagtatgc ctgtgcattt ttattgccaa aagagagttt tttaaaagat ttgactcatc
    10081 cagaagattt agattattat gtagaactaa agaaaaaatg gattgtacct atatctgcta
    10141 tgatttttag agcatataat ctagaaaaga taaattacaa aaaatacaat tatcttatga
    10201 atgaaatgga taaaaaaggc tggttgatag aagaaccact agacaaaatg aaaggttcaa
    10261 gcccaacgta tttaaaaaga gcagttgaat tattgataga aaataaaata atgagtgtaa
    10321 atagtatagt atcaagttta gaggaatttg gaataaatct ttatccagaa gacttagaat
    10381 tattgatggg attgaaaaaa ggattgttat ctcaagaggt aaataaaaaa agtaaagtta
    10441 taaaatttga tggtaaaaaa taggtttata ttttagtatt aaaattaaaa gcaggtacaa
    10501 atgatttaaa cattaactta gttaaaggtc ttttcaataa gtcatttacc tgcttttagt
    10561 gtaagaaaga atattgaatc taaagtgttt ttgaaagtgc aatatattcc cttaaaacag
    10621 aacaagattt attaaattca ttttgctcat attcagtcat attgatttca ataatttctt
    10681 caataccatc ttttcctata acacatggaa cacctgcaaa tacatttctt tcgttatatt
    10741 gaccttctag taatgtagaa actggaagaa cttttttctc attgtgtagt atagctttta
    10801 caatttctac taaagcagta cctataccaa attctgtaca atctttacca tttattatac
    10861 ttatccctgt ttttttattt ttttcaacta ttgatggtaa gtctaaattg ctataagtac
    10921 ttgggttgtc ttttatcatg tctaagattg gtttaccacc aattgaaaca tgagaccaag
    10981 gaaccatttg agaatctcca tgttcaccca tagaataagc ttgaattgat tgttgagcga
    11041 ttccagtttc ttcagataaa atccttctaa gtcttgttga atctaaagct gttccagttc
    11101 caatgacttt gtttttagga aagccagatt tttcccaaat ataatttgta actacatcaa
    11161 caggattaga tatgactaca aatataccat caaagccaga atcaactatg ggttttacta
    11221 tagattttat cattccaata gttggtctta gtaaatctaa tctagttgtg ttttgtctac
    11281 gtaaaggccc ttctgaagag tctgcaacac ttatcactac tatgtcagaa tctttacagt
    11341 cagaaaaagt tcctttttct atatgtactt tatgtggcag atatgaaact gcatcagcta
    11401 aatctaaagc ttgagctttt gatttagatt catctatatc aatcataaag agttcatcac
    11461 aaactccttg agttattaaa ctaaatccac aatgagaacc aacatgccct gaacctacaa
    11521 tagatatttt tcttggcttt attgccattt taaatcactc cccctaaaat aacgatacat
    11581 tgaaaattat agatgttatt aaaagctgtt tttggatatt ttaatattct attatttcag
    11641 aaaatttatt taatatgatt ttatactaaa atttttaaaa aagcaatgtt aatatactct
    11701 ctattagtga atattaaagt attatatgta tattaataat tattaaaata tgtaatgtca
    11761 ctgatagtta gaatatttaa gtgctaaaag atgtgtataa ataataatga tgtcagttta
    11821 taatgatatt aaattgagat atactgtttc tctaaattaa aaaataaagg agtaaatgtg
    11881 ttatgaatta tcatacaaaa gaagaattgg tggaagcact gagagtagta tcttcaagta
    11941 ttattaattg tggaaaagga cagaagaaat tttctgagga gacatctcat catacacgtt
    12001 ttaagaatat tatagaagta atgtatattt caaaatcatt aataatggat gaaattagta
    12061 aaagagattg aagcttattt tgcaatttaa catgttttaa tatacatttt attatataag
    12121 aaaataagta aacatacatg ttataaaata ttgaaataat atacaattat aaatttgtta
    12181 acattaatat ctatatgtta aaatatagta gaagatgtta gtaaataata ggatgagttc
    12241 tattttaata caattaaatt aagcttagtt tgaaatttgt atctaaaaag taaagtaatc
    12301 tagaattaag aaaatggaga tagaattata atggaaaaat tatattatac aaatcagtat
    12361 ataagagatt ttacagcaga aataattgaa ataaaagaag tagaaaataa gtatcatgtt
    12421 ttattagata agacagcttt ttttcctggt ggaggaggtc agtttggaga cctgggaaaa
    12481 atagagaatg atgatgtaat aaatgtgtat gaagatggaa aaaaaatata ccatgtatta
    12541 gataaaaaac ctataaagat acataaggta aagtgtagta ttgattggga tagaagagaa
    12601 gatggaatgc atcaacattt tggacaacat gtattgtctg gatgtttttt taaaactttt
    12661 aatgcaaata cgacaggttt tcatttggga aatgagtata gtacagtaga tatagaaggt
    12721 aatttagaag aggataaaat tagagagata gagttgtttg ccaatgaaat tattcgtcaa
    12781 aatatacaag tagagacatt acttccaacg aaaaaagaac taaaaaagat ttggttaaga
    12841 cgtgatttgc caaatactga tgaagagata agagtaataa aaataggtga tttagatacg
    12901 aatgcatgtt gtggagtgca tccaaaatca actggtgaat tgagaatgat aaaaataaag
    12961 aaatgggaaa aacataagtc aagtacaaga atagagtttc tagcaggtaa aagggcagtg
    13021 gatgaagtat tgaagaggga tatatatttg actaaaatat gtagatattt aagctgtagt
    13081 gaggaagatg ctataaatgg tattgtaaat ctaaatagta aagtggaaga ggctttaatt
    13141 aaaaaaagaa ggcttgaaga agtagtatct acatatcagg tcaaagaaat gattgaaaaa
    13201 gcaaataagg ttggcaatat ttcggtcatt aaaaaaattt atgatgatga agatttgaaa
    13261 tatgtaaata agatagcaaa taaaattgca gaatatgaaa atagtattgc attattagct
    13321 gttaaattta atgataaaat aaatttagtt tttgcatgtt ctaaaaattt aaaagatatt
    13381 aatatgggag ttttattaaa ggatgcaata aatcttatag atggcaaggg tggtggaagt
    13441 aaagtattag ctcaaggagg aggtaagaat aatggaaatt tagaggctac acttgattat
    13501 gcatttatga agatagaaaa aactctataa atatattata taaaaaacct tagtagataa
    13561 aatagatatg gaggaataag tatggagcaa caaaaggact acatagagtt atatgaaaat
    13621 gaagaactaa tagagataag agaagcacgt atggatgatt tggatacaat agcaaaattt
    13681 aattataatt tggctaaaga aactgaagga aaagaacttg atatggatgt tttaactaaa
    13741 ggtgtaaaag cattgttatt agatgagaga aaaggaaaat atcatgtata tacagtattt
    13801 gataaagttg ttgcacagat tatgtataca tatgagtgga gtgattggag aaatggaaat
    13861 ttcctttgga ttcagagtgt atatgtagat aaggaatata gaagaaaagg tatctttaat
    13921 tatttattta attatataaa aaatatatgt gataaagatg aaaatatagt tggaatgaga
    13981 ttatatgtag aaaaagaaaa cataaatgca aaagcaacat atgagtcttt aaatatgtat
    14041 gagtgtgatt ataatatgta tgaatacgaa gtagtacatt cgtaatataa atttgtaata
    14101 ctgttatact gatgttataa agtattgatt atattgacga ttatatgaaa taaactggat
    14161 attataatta gaatttttag ggggtataaa aatgggcgtt ttaaaaggaa tgttttatgt
    14221 atttttattt ttagatttag tgtcaatatt ctgtttcttt ttcaataaag gaaaaatagc
    14281 atctaataaa attgtgttta atgcaatagg agtacttaca tttgtacttt gttttatgct
    14341 ttttagttat tatcctaata ataatcttat tggaaaattt attgcttctc tattttttat
    14401 atttggagtt gcaggagtta tgttgaaaga aaaaaatttt ttatatgcta gacttctttt
    14461 aactgtagtt atagtttttt ctacattaag attgttcgtt attcaataaa atgtatttgt
    14521 ataaaaattt attaaagtct acacatttaa gtgtaggctt ttttgatttt ttgtttgaaa
    14581 attaaggaaa ttttaaggaa attttaaaat cgtcttaaat atttattgat atacttaaaa
    14641 tattaatagc gaaaggagac ttatcagtga tagaaagtaa aaagtcagaa aaattgttac
    14701 tgtcaattat atacataata tttttggtat atattttaat gattataaga ataattttat
    14761 ttagagatgt accaatttac gctattttca agggaacgtt taggaatgta aatttaattc
    14821 cattttatac aatatatcaa tttatagtag atagtaatct tgactttatg aaagctactg
    14881 taaatattat agaaaatata ggtatattta ttcctatggg aattttttta cctatagtat
    14941 gtaaaaatct aaataaaaaa actataatta tagtaattat acttgttagc ctagcatttg
    15001 aattaacaca atatattttt gctcttggaa gtagtgatat agatgatgtt attttaaatt
    15061 cattaggtgg cataattgga attaatattt acataaacat gaataaatta tttcccaatg
    15121 atattaacag atttaaggct ataataggta ctagtttagt tttaggagtt ataggattag
    15181 gagttatttc taaaaattat cataacttac taacttttaa atttagacct gatagaaaaa
    15241 tatcaaaaat attaattgaa gaaaataggg aaataattaa agatataaat aaagatacag
    15301 tagatatagt tgggacattt gagagcttta aaaaaggaat aataactatt aaagcaggtt
    15361 ctaataacaa agtaaagaga ccagagaatt taattgattc tgatggaaat ataagaattt
    15421 atttaaatga aaatacaaaa ttagtatctt atataattac caaagaatca aaaatggata
    15481 tagttaaata tgaggaattt gatgtgaaga atttagattt attaaggaga tatgatacta
    15541 ttaatgtttg gattgataaa gaaaatcagc caaaggataa acatggtatt atggctagta
    15601 aattattaat tggattgtat gaataaaata ttaaacaaga ataaaagaat aacaaatata
    15661 aaaaacgaaa aaactatttc tataattatt tttacgttgt gcataaaata agaacattag
    15721 tgaaggagta gagataaatt atggagataa taaaatatat cttgtacttt tttatatatt
    15781 catttttagg atggacagta gaaagtatag gatgttctat agcaagcaaa agaataatca
    15841 acagaggttt tttaaatggt ccaatatgtc ctgtttatgg ttttggagcg gtaattgtga
    15901 tatcactatt gggaagattt aataatgtag taatagtatt tctattagga atgattttaa
    15961 ctacgatact agaatatttt acaggattta ttttagaaac cttatttcat gctaaatggt
    16021 gggattattc tgacagaaaa ttcaatataa agggtagagt atgtttaaaa aatgcaatat
    16081 actttggtgt aatgtctgtt ttaataataa gatttataca tccatttata aaatattttg
    16141 taagtataat tccatataga atattaattt caatggctat aattacaaca ctttggacta
    16201 tacttgattt gattgttact attataacac ttaaaaaatt agatataaag ttaaacttac
    16261 tagacgatat aattacagac cttaatgata taaatgtgaa attggataaa tttgatagag
    16321 gagaaataca agctttattt aaaacaataa ataacgacga attagaagtt agagaaaaaa
    16381 taaacaaaat aaatagtaag ttggaccgta tagagtctaa tattatttta caaaaaagag
    16441 ttataaaagc ttttccacat ataaaacata aaaaacaaca agagcaacta gaacatttta
    16501 aaaaaattat taatgagaaa aggtaattat acaatttaat gaagacgaat agaataatgg
    16561 tatttataag atgattttca gaggactcta taaaaataga atagagtcct ttattttttg
    16621 aaaaataatg tttatttgat aaaaataaaa tatattgttt ctgaataaca aatattttga
    16681 aaattataaa aacatatatt gacaatttgt gtcaaaaata ctaacattaa gtatataaag
    16741 aattttatat gagaatatct ttataaatcg caataatttt aaaacataaa tttatttaaa
    16801 gaggggatga tttgccaatg agcgagataa ctagtcaaat gataagtaca gaagaaaaaa
    16861 aatatgttgc taaaactcaa aagatacctt attatccagt agcatttaaa tcaggtgatg
    16921 gtgcaatgct atatgattat gaaggtaatg aatatgtaga ttttttagca agtgcaggct
    16981 ctgcaaatgt aggtcatggt aataaggaaa tctcacaagc agttaaagaa caaatggatg
    17041 atataacaca atatacactt gcatatttcc atagtgatcc tcctgtaaaa ttagctgaga
    17101 agcttgtgga aatagcccct ggagataatg ataaaaaagt actatacagt gcaactggtt
    17161 ctgcttgtat agatgctgct ataaaattag caagaggata tactggaaga acaaagataa
    17221 tatctatgtg tgaatcttat catggaagta cttatggagc tatatctata tctgctttaa
    17281 gtacaaatat gagaagaaaa atgggtccat tgttaccaga agtttatcac ttccattatc
    17341 ctgataaaaa tagaacagct aaagaatgtt tagatgagat agaatatgct tttgcacact
    17401 atctaccagc agaagaggta gctgcaatat ttatagagcc aattgcagga gatgcaggaa
    17461 taatagtgcc tccagtagag tgggtacaag gtttaagtaa aatatgtaaa gaaaatggaa
    17521 tattacttgt aagtgatgaa atacaacaag gtatgggaag aacaggtaag tggtttggta
    17581 tagaaaattt tggtgttgaa gcagacctta tagttcttgg aaaatctgtt ggaggtggat
    17641 tacctctagg agcagttgtt ggaagaacag aaataatgca aagtcttgat gcaccagcac
    17701 atttatttac tttagctgga aacacaacag tatgtgtggc tgctttaaaa tctatagaga
    17761 taattgaaaa agaaaactta cttcaaaaga gtatagaaat gggagattat attaaagcag
    17821 gatttgaaaa actaaaagaa aaatacgata taattggaga aataagaggc ataggattat
    17881 ctataggagt tgatatagtt aaaggaaaag gttctaatga aaaacatcca gatgctacag
    17941 ctaagatatg ttatagatgt atacaaactg gattaataat gatattctta ggacaatcta
    18001 ctttaagagt gcaaccacct ctagtaataa ctaaggagca agtagataaa gcaatgaaca
    18061 tcatagattc tgcaatagat gattatttaa atggtagaat tggtgatgaa gtatatgaag
    18121 taactcaagg ttggtaaaat aaatattaaa tgaaaatagg agttatctca attttagaaa
    18181 attatttctg aaatgagata actcttttaa gttacccaca aataagaaaa ttgtttgatt
    18241 cattaaagac tatatttcgg atgtcgtttt tgtgagtatc agagacagta ttacgagaat
    18301 aattaccttt attcgtacta tctgttaatt tagttataat taagatagct aaaagaataa
    18361 gaattactgt gtataaagca ataacatatg gttttctttt atatttgtat ttgttattga
    18421 aaaaagaatg gccataatta tttttattta ctttagatgt atttgtattc tttttcttgt
    18481 tacctttctt tttatcatcc ttttttctat ttttagggta aggaatgact ttgtgtgtat
    18541 tatcaagttt gatattttta tttttttgaa tattgttttc caggctagcc tttttttctc
    18601 caaattgaat agttttattg gtattgttgt tatctttcaa tgttaatctc cctcccttaa
    18661 actttccctt tagataatat tactactaac ctatggaata tacaataaat aatatataag
    18721 taataaaatt ttataggtat aaatctcttt ctccacgctc taaggcagtc atattgttag
    18781 atactatgtt tcttatatct tctctttcta tcttacctat ttcttctgcg ataagttttt
    18841 tacccataat tttaagttct tcatctccat aatcgattaa aaattcattt aatgtagtaa
    18901 gagcatttgg tgtacataca ttatggattt gtcgactttt agctaagctc atgaatctat
    18961 cgccagttct acctttacga taacaagctg tacaataact aggtatatat ccatcagtaa
    19021 tcagttcttt aagcacctca acagggcttc tatggtctcc aacttcaaat tgttctgtat
    19081 tgtcaccatc ttcatatgct ttatatcctc caacacctgt tagagaacct gcactaattt
    19141 gtgatacacc atattttagt aactcatttc tcatttcagc agtttctcta gtggacatta
    19201 tgattccagt aaaaggaaca gccaatctag ttatagctac tatttttttg aacatatcgt
    19261 cagaaactaa gtgaggaaaa tcttctaagc tcataccttc agcttttttc aatctaggga
    19321 aagatatagt atggaaacct acaccaaatt tttcctctaa gtgttcgttg tgcatcataa
    19381 gtcctagaac ttcaaattta gggtcagata aaccaaataa aactcctgca ccaacatcat
    19441 caacaccagc ttccatagct ctatcaaaag ctgttaaatg gtaataatag tcatttttta
    19501 tagattgacc atgcattttt ataaatgttg gtttgtgata agtttcttgg aataatatat
    19561 aagttcctat tcctttttct tttagtaatt tatactcatc tacacttgta gctgctatgt
    19621 taacgtttac ccttcttata tttccgtttt cattatatgt tgaataaatt gcatctaagc
    19681 aatctaagat gtaatttata tcacaatttt tagggtcttc accagcttct agagcaagtc
    19741 ttttatgacc cattttttct aatattttaa cctcttcctt tatctcttcc atggtaagtt
    19801 tttttctttt aaatttgttg catttactaa aaccgcaata aacacattca tttacacagt
    19861 agtttgatac ataaagaggg gcaaataaaa ccactctatt accatatatt tcatttttta
    19921 tttgcccagc tatactaaat aatctatcta gttgtttttt atcttctact tcaagaagtg
    19981 tagctatatc tttataacta agtttttccc ttctatcagc cttatcaagt accttttcaa
    20041 tatcatcaga agttgaattt tttgcatctt ctaataaaga attaataagt tcatggttta
    20101 tgaacattta caaacgcctc cattcaatat tatcaccgcg agaatagtca acttcaagtc
    20161 ctaatgactt gatgttttct tctaaacttt ggtgatattc acaagcttct tgaccagtaa
    20221 aggctttgtt atcgtaaaga gaatattgtt ttctaaattc ttgaggagaa aggtttggca
    20281 taattacatt gcaaccagct aaaagacctg catttctacc actagggttt atagatgcca
    20341 aggctgttgt agcgggtaat aaaacttttg gaagtagaag tcttgtaatt gaaagcataa
    20401 gtaatgtctt ctctaaatct ccatgtttat aatccttaaa aattgtatca tggtgtggaa
    20461 taaaaggtcc tattccaacc atatgaggat ttaactcttt taggtataat aaatcacgaa
    20521 caaggtcttc gttgttctga aatggtgatt caaccataaa tccagcacct acctgataac
    20581 caatttcttt aaggtttttt aggcattctt ttctagttct aagctcaatg tttggcggat
    20641 gtaatttttt ataatgactg tctgtagcag tttcatgtct taaaagatat ctatctgcac
    20701 cacataaaaa gtatttttta tacgaatcat aactcttttc tcctagtgat agagttatgg
    20761 cacaatcctc atatttattt tttatacttg aaacaatttc acatatttta tcatcagtaa
    20821 aatatgcatc ttcaccacct tgcaaaacga atgttctgta tcctatttta taacctatat
    20881 cacaacattc aagtatttca tcaagtgaaa gcctgtatct tatgatattt ttattagaag
    20941 attgtattcc acaatagtag caattatttt tacaatagtt tgtaagctct ataagtcctc
    21001 tcaagtagac tctatttcca taatgtttat ttctcatgtc gctagcttgt ttataaagat
    21061 agtttcttaa attttgttcg aagttatatt cctcattgtc gatattttct attaaatata
    21121 gtagttcatc aaaacttaat gaattaatgc tgtaaagttt atctataaag tatttaatct
    21181 tatttttagt catattcaaa tatgtaatta ttaagtatag ttaaattttt aataattgaa
    21241 aagttcaagt gatctttcca aaataccttt tacatatgct attaagacac catagtttac
    21301 aataggtaca ttgaaagatt ttgccttttc tattctagat aacattccag ctctattcat
    21361 catacaagct ccacagtgaa ctacaagggc atatttattt atatcttcag tgaaggatac
    21421 tccagaacta aattcaaaag taatgttttt gccagttttc tttctaatca tattaggaat
    21481 ttttactgtt ccaatatcat cagtttgtct atgatgggta catccttcgg ccatcaagat
    21541 tttatcacca tcttttaaat tttctaaagc ataagctcca ttaatcaatt ctttaaggtc
    21601 tcccttttgt ctagcaaaca atatagaaaa agatgtaaga ggaatatctt tttgtgtatc
    21661 tttatctact tgtggaaata cttgagagtc agtaataaca agttttggtt ttttgcctaa
    21721 attagataga gtttctttta aactatcttc ttttgtaact atagaaattg caccattatc
    21781 caaaatatct cttataactt gctgttgagg aagtataagt ctaccttttg gagctgcttt
    21841 atctattgga acaactaaaa caactaaatc atttggttca attaaatctg ataccaattt
    21901 gaactcagtg ctatccttag gcaaaacttt tattatttca ttttttaaat tttcaattcc
    21961 aattttatct gttgatgaga cagaaacaac tggacattta actttatttt tagttaagtt
    22021 taaaatttct gattgattct taatagtatc aattttgtta agaattagaa tatgagggat
    22081 attcttatca ttaaattttt caattagtga caaatcttct tgtgatatac caatttggca
    22141 atccactact agtaaagcta tatctgtctt ttctaaaaca tctagtgatt tacctattct
    22201 aagttcacca agttcaccaa catcatcaag accagcagta tctataagaa cacaaggtcc
    22261 tataggaagg atttccattg gtctaaatac tgggtctgta gtagttccag caatatctga
    22321 gactattgca gcactttgat ttgttatagc atttattatg ctagatttac ctgcatttct
    22381 tttaccaaag agacctatat gtactcttac cgattgtgga gtagaattta gactcatttg
    22441 tactcaccac cttaaacttc tttttttgat atactagttt ttacagatac atgaggtatg
    22501 ttgcccaatt taccagtcaa actattaata gtgtccattt cgccaacaac tgctatacat
    22561 acgattgaga caccttcttc ttcgcaagga atgcccattc ttcctttaat aataccttta
    22621 aaacctgata caacctcatt gaatttaaat tgacattctt ttggttcttc caaaatagca
    22681 cttataactg caaccttttt cataaatatc ctcctaactc agaaaatatg tcttaaacaa
    22741 atccaaaatc aaaaaacttt tctttgtaaa gaaaagtttc tacaaaagaa aagttattaa
    22801 ttacacaagt ataatgtgct atattaaact atttccttac agattagata actcttttct
    22861 ggtagggtta gcttgtaaaa atttaattta acaacaagta actgaagaaa atttttatac
    22921 aatttatata aattataata aaaattaact aaagaaaatt gaagttactt atataatagt
    22981 agtaaattga aattaagtct atagaaaaac caatttttgt ataaaaataa aaattaaagt
    23041 taattatttg ttaagaattg actttggtaa aatatataat taattattct ttaagcatat
    23101 aatttataag taattttagt gtattttcaa tagcttctat atgtgttctt tcataatggt
    23161 gactattgtt tgttccagga ccaacacatg ctatttgaac atcttctccc caacgaatag
    23221 cttctgaagc atctgaacca tagaaagtaa atacatcgac agcgtagtct aaattattgt
    23281 cttctgcaac atcgacaagc ttgtttctta aattaaaatc atataatgat aaattatctt
    23341 ttgcaactat agttacacca tgttcactag atgtttgact tggtccagta ggtgctatat
    23401 ctattgctac aaattctttt gttttttcag gtaaacattt agaaacacca tgtccaattt
    23461 cttcataatt acttatataa aagttagtag tatgcttagg tgttaaattg ttttctttaa
    23521 aatatctaca tatttcaaga accattgcta cagctgattt attatctata tatctagatt
    23581 ttataaaacc agattctgta gcctcaaatc tatggtctag gcatacaaaa tccccaacac
    23641 ttattcctaa atctaataca tcttgctttg taaatacttt ttcatcaagt ctaataatca
    23701 ttgtttcttc atttctttca gtggcagctt ttgtttgacc atatatatga gtagaagcat
    23761 acttaaatac aacacttcct ctgatttttt taccttttct agttataaca gtacagtttt
    23821 ctccctctat agccgcccaa catccaccac caactttatg gtatttaaga gtaccatcag
    23881 atgatatttc ttttactata gctccaagtg tatccatatg tgcagatatt gttatttgat
    23941 ttgaatcatc ttcaccagga agagtagcta taagtgcacc tttttttgta agaatagtgt
    24001 caagacttaa attttcaaaa tcttttttga tttctaaaac tgcattttca gtatatccag
    24061 ctgggcttgg aatgtccaaa tattttttca ttaaatttat agtattagtt aaatttaaat
    24121 tcatattgtt tcctcctaaa taaatatatt tatacaaatt ataacataaa tacaaatgtt
    24181 ctgaaaa
//
LOCUS       DAMJVK010000058        23800 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.059, whole genome shotgun sequence.
ACCESSION   DAMJVK010000058 DAMJVK010000000
VERSION     DAMJVK010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23800
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.059"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            301..471
                     /locus_tag="PC414_002372"
     CDS             301..471
                     /locus_tag="PC414_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464762.1"
                     /translation="MSALLYGVCNKSISVSIVINMQEFKIGVSMAYGVLVIGICYIAL
                     GIIFKLDRKRFT"
     gene            1014..1892
                     /locus_tag="PC414_002373"
     CDS             1014..1892
                     /locus_tag="PC414_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HDF2464763.1"
                     /translation="MLINMKEMLKVAQENQFAVPAFNIGSGQILKAVVQSANEKNAPV
                     ILAIHPNELSFLGDSFVASCIEEANKSKVPMVIHLDHGENKEQILRAIRCGFTSVMID
                     GSHLPYEENVAISREIVEIAKGLNVSVEGELGTIGTTGTSSEGGTDEIIYTDSKLAKD
                     FVEKTGVDTLAIAIGTAHGIYPKGFKPELKLDLLKEIREVVDIPLVLHGGSSNPDEEI
                     AQAVKLGVCKINISSDVKSAYYKKCRELLEQNPSLYEPDTIYPPCIKAAREVIEFKMN
                     LFNAIDKLKCFYKNKN"
     gene            1928..3823
                     /locus_tag="PC414_002374"
     CDS             1928..3823
                     /locus_tag="PC414_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2464764.1"
                     /translation="MIGKILRSDCIVLDKQLQTKKDVINYMANHLYHGNYINNKEKFI
                     ADINKREKESSTNMGNLIAIPHAKSNVVNQPVMMYLRTDREITDNDGQKLRSFFMIAV
                     PESGSDEHLKIISKLATYLMEENFLKHLLSAETPYEILSIFNNKAAESGDFSNNQNKG
                     LIVGITGCPTGIAHTFMAKKALEEAGQELGYEVKIETHGAIGVENKLTKEDIKRAEYV
                     IIACDKEVQLSRFIGKKVLQKKVVDAKNVSLAKNLIINAENGMAKEIKPGGKTLEDSK
                     EMLQGSEKKGVYKHLMSGVSEMIPFVVIGGIGIAIAFMFGIFASDPTHETYNPIAGFF
                     GQLGGDSAFKLFIPILAGFIAKSIAGRQGFAPAMIAGFMAVIGGSGFIGGMVAGFATG
                     YIALGVMKITENLPDVLKGVNAVLICPLLIAFTSGAIMFFVVNTPASWLNEALQSWLQ
                     SMSGTNKVLLGAILAGMMASDMGGPINKTASAFGLAMFSSNIFEPSAALMVGGMVPPL
                     AISLAMLIFKNKFTKEERKSSLPTAIMGASFITEGAIPFAAADPLRMIPANIIGSCIG
                     GAICMSLDITLMAPHGGIFVIPFACSAPVAYIACILIGTVITALIIGVTKPTIRSENS
                     EVELLGA"
     gene            4091..5998
                     /locus_tag="PC414_002375"
     CDS             4091..5998
                     /locus_tag="PC414_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2464765.1"
                     /translation="MLVSRQIEFLKLILNENKYRPIKYFKDKLNVSDKTLQKDLKMIE
                     KYLETFNIKIDIKRGYGILIEDLAKKNIDLINSLNIQSKENKSLSVEQRRMEILKYLL
                     LNSNNITSINQLADKYYVSKTSIVNDFKYIEAWIKEYNLKLNKTLEGTKIIGKEVDIR
                     KSIAKMMDDLLDENYEQHDISELTRLDSATFSALINLFDIDSIIFVETIITELEYNLG
                     YTINQPYYINLITHILICLKRIEEGNQIESQEEREISVDNLDELVYKNVTTLIDKIEN
                     RYRVKITKDEIMYIYIFLVSSGFSSENNSSYNDESDRLSESEKVSSIMIENMSRFLNI
                     NLKNDDLLQKSLASHVRPMLNRLRYDIQIKNPLLGEIQERFSDVLGLCMMTINIMTEY
                     DNLKNISIDEVSYLATYFQAAIERNMSSKRVIVVCHTGYGTSQLLAARLKREFPEWTI
                     VDIVPMHQLSKRNLDDVDFVISTVNLDLKDKLHIVVSVLLMESDINNIKNALLSKPKK
                     SMNLDTNLLGMYLEDNIYFNFDVAQMESLIGVNLGSKYESISLGKDLNIYIHKQSKLN
                     KGIMNINNVTRTIDIYLEISNQSFLKNILMEISNLYRQKNYINYIIDCKSKEDIKILF
                     KNNLLEKTNGN"
     gene            5988..6437
                     /locus_tag="PC414_002376"
     CDS             5988..6437
                     /locus_tag="PC414_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2464766.1"
                     /translation="MEIREILTEESIILDLKSTTKEGIIQELVDKLYDTGSITLKSKF
                     IKALKHREKIGSTSIGYQMAMPHGKTKYVKKASIVIGKSKKGVDWPSIDGLPVKVIIM
                     FAMPQSGGNEDMILISKIAYILGGKNIGERLLNAHTKKEILDIFYEV"
     gene            6537..7424
                     /locus_tag="PC414_002377"
     CDS             6537..7424
                     /locus_tag="PC414_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2464767.1"
                     /translation="MAIYLEMPKLIHEFPFRTLSNEGEVLTTPHWHKEIEILLITEGV
                     VNLFINDKPMQLKKGEIVIIKGGDIHYILPSPGSERLVFQFDISFFNDLILLNEEKES
                     LINLFSSIKKCSRDWSAKIRKSVFDILIDIYNEDLYKIEGYSYAIKGKMYNLIPILYR
                     QIPREIEENTVEYEINSKEILERLNNIFKYVEKNYKQPIKLEDVSYEVGFSVYYFTKF
                     FKKNTGKTFITFLNEYRLEKAKWILLNNNISIVDLVEEVGFGSTKTFYRVFKEKIGVS
                     PLEFKKRYNSLEKKGSVSK"
     gene            7829..9277
                     /locus_tag="PC414_002378"
     CDS             7829..9277
                     /locus_tag="PC414_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HDF2464768.1"
                     /translation="MKLGKDFLWGGAISANQSEGFYLDGGKGLSQFDKLPLNDKRLKN
                     VCLDEDNILNENNQYYPSHIGIKFCENYKEDIAMLAEIGFKCFRFSISWSRIFPTGEE
                     SEPNEKGLQLYDNIIKELKKYNIEPLITISHFDIPYSLVEKYSGWADRRVVNLYVKYA
                     EILMKRYKDDVKYWIPFNEMNMIMHIPYIGAGLTFIADENQLQKKYQAAHHQLLANAK
                     TIEIGRYINKEFKFGCMLAAGKTYPYTCKPEDVFATLEHERHNLFFSDVQVRGVYPSF
                     MSYYLKSNQIKLIIKEEDLSTLKDNTVDFVSFSYYSSACSSARAEGLEKSKANGPETI
                     KNPYLPKSNSVWQNDPVGLRITMNQLYERYQLPLFIVENGLGTQDIVNEDGKIIDDYR
                     IDYMKEHIKNMILGVNEDGIELIGYLMWGCIDVTSVSEGKMTKRYGIIHVDSDDYGRG
                     SFKRTKKQSAFWYKKVIETDGEILFNDMETHA"
     gene            9303..11693
                     /locus_tag="PC414_002379"
     CDS             9303..11693
                     /locus_tag="PC414_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HDF2464769.1"
                     /translation="MSLIFTTKDQLVELGFSKDTIKSNETLFTLANGHLGVRGNIEES
                     DFNNEYVDNMGTYVNGFYEESPITYGESAYGYAKQNQTICKLPNAHIVNFSIEGDCFD
                     LSKGITSEHTRILDLNEGILKRSFIWENSRGRKVKVSIERLVSQDIEELMMISYTITP
                     LNFNGKIKFKNKMENSMEKFNCEKVDDPRVASINKKQFKINVKKYNNKYFMNLVTERS
                     RLGLWCGFDSYITCGNITNETYNVSDECIEREIEVYAKKDERLNLEIVVGYSRVYSDL
                     NLNEERLKSLECLESLKCLESEAHLKSLELVLREACNIGFDKVKNIQRKKMNEFWTTS
                     DVEIEGDKTLQLGIKTNLFHIYQSAGRDGKTNISAKGLTGDGYEGHYFWDTEMYILPF
                     FIYTQPSIARALLDYRYSILDASRERARELGINKGCLYSWRTINGNECSAYYPAGTAQ
                     FHINADIAYGVRTYFEATNDEEFMMQNGLEILIETSRFWVEFGDYIESKNNKFCINGV
                     TGPDEYTAIVNNNYYTNLMAKYNLIYAVQMVDKFKHNLFSNEVFRKVKLQEYEVKNWI
                     NAAQNMYLPYDEEKKISKQDDSFLEKSVWDFKNTPKEKYPLLLHYHPLTIYRYQVNKQ
                     ADTVLSALLFPDEFSLEQKKRDFEYYEKITTHDSSLSRSIFGMMASEIGNHEKAYNYF
                     MDTALMDINNLQKNTSDGIHAANMGGTWMSLVYGFAGMKIKNDKLSFEPRIPKHWKNI
                     KFNILFRDNLVFVNIEEYKSYYKLIKGRNITILHDNKEIKLSKNIIEVNNCFKGSN"
     gene            11695..12366
                     /gene="pgmB"
                     /locus_tag="PC414_002380"
     CDS             11695..12366
                     /gene="pgmB"
                     /locus_tag="PC414_002380"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDF2464770.1"
                     /translation="MKAVLFDLDGVITDTAKYHFEAWHEIAKSLDIEIDETFNEKLKG
                     INREESLDKILLSANKVDSYTKEEKLKVCEEKNNIYIKLINSLTYKDILPGINELIKE
                     LKKNNVKLGIASASKNAPRILESLGVKDEFDCIVNPSLLKRGKPYPDIFIEGSKMLNL
                     KQYDCVGIEDSYSGIKAINAANMTSIGVGKKEVLGKCDHIVEDTSLLNYNTLLQAWSK
                     KINLD"
     gene            12405..13775
                     /locus_tag="PC414_002381"
     CDS             12405..13775
                     /locus_tag="PC414_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2464771.1"
                     /translation="MKNKYEDVAQLIIDGLGGKDNIIDLTHCMTRLRFILKDEKKVNA
                     DKLKSIDKVVGVNSTSTQYQVIIGNEVGVVFNAIINKGINTTGDIKSDIKNKNKKNSI
                     LNNIIDTITGCMTPMIPALTASGMIKVILSLCTTFNWLSSNSSTYRVLDIIGDVAFYF
                     MPIILAINAAKKFNVNTSIAVIVVGVFLHPNFSAWVSSGDPISFIGVPIQGVIYAASV
                     IPALLTVWMMSYIENFIDKLTPSMLKTILNPTLVLLISAPIALIVIGPIGNFLGEGLA
                     SIINLLQGRLGFIMVCLLAAAMPFIVMTGMHHALTPIFISAFATTGQESLILISQVCA
                     NLAQGGATLAVALRSKDSKMKQLASAAWISAIMGITEPALYGVTLKLKKPAMLASIGA
                     GIAGLFAGITHVTLYVPQNSIMAVLGLSGEKGISNIVNGLIVIFIALVAPFIMTYVFG
                     VEKNVK"
     gene            14122..14964
                     /locus_tag="PC414_002382"
     CDS             14122..14964
                     /locus_tag="PC414_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2464772.1"
                     /translation="MIINKILNNNVVITLDDNDEEVIVMGKGIGYQKSKGNLIDKTKV
                     NKVFRISNKEISNKLQELLNNIPIEHMKLSSEIIEYAQIKLNKKLNESIYISLSDHTY
                     SAIQRMKEGINVKNAILWEIKRFYKEEFEIGMKALDIIENKTGIKLPEDEAGFIAFHI
                     VNAQLSEGHTLASDITKLIQEVLSIVRYHFRIEFHEESVFYYRFIMHLKFFAQRLLLD
                     SAHEGETDKELLSIIKSKYNKEFECVVKIKNFIKKQYNYILTDDELIYLTIHLAKVVK
                     DSNM"
     gene            15109..17037
                     /locus_tag="PC414_002383"
     CDS             15109..17037
                     /locus_tag="PC414_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2464773.1"
                     /translation="MGKYKQLAQEIVNNVGGKENISGLVHCITRLRFTLKDESIANDN
                     VLKNMEGIVTVMKSGGQYQVVIGNHVEAVYKDVVEIIGLDDSSTSSETKKSGNILDKG
                     IDIISGIFQPVLGIMAACGMLKGFNALFVAMGLYSDVSGGYMVINAAGDALFTFLPLF
                     LGYTSAKKFGLKPMLGLALGAAVCYPAIQGSSISAGADVMYTLFKGTMFASPVYIDFF
                     GLPIVSIDYTGTVVPIIFIVYFASRCEKLFNKCVPNLVKFFFVPMLTLLITVPVALIV
                     IGPIATFGSTLISQIVISIRDFSPLLAGAIVGFTWQILVIFGMHWGFIPVYINNVMTL
                     GYDNVMMPFFACTFASSAVVLAIFFKTKDKKLKEMAIPNFISGIFGVTEPAIYGILLP
                     LKKPFIISCIASGIGGAFYGFFNLRKFITGGMGIFELPAMIEPDGGMGNLIVALSGIA
                     ISMVVAFVLTMILYKDEKVEESKKVRNKGKEEIKEVKSTKLEREIVTSPIKGEVLKLS
                     DIEDAAFASGVLGQGVAIIPSDGKVVAPVDGVVTTLFPSLHAIGILSDTGVEVLIHIG
                     LNTIQLEGRGFEACIKQGDRITKGQTILNFDVDAIKELGYSTVTPIVITNSSQFLDVV
                     ETESKNIELEDNLITVLF"
     gene            17098..18525
                     /gene="ascB"
                     /locus_tag="PC414_002384"
     CDS             17098..18525
                     /gene="ascB"
                     /locus_tag="PC414_002384"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDF2464774.1"
                     /translation="MSFKNDFLWGGATAANQCEGGYNEDERGLANVDVCPTGKDRTAV
                     ITGKLKMFDFDDEHYYPAKTGIDMYHNYKEDIKLFAEMGFKVYRMSIAWSRIFPKGDE
                     ETPNEKGLQFYENIFKECKKYKIEPLVTITHFDCPMHLVKKYGAWRNRKMIGFYEKLC
                     NVIFRRYKGLVKYWLTFNEINMILHAPFMGAGICFEEGEDVEKIKYQAAHHELVASAI
                     ATKIAHEVDSNNLIGCMFAAGSVYPYSCNPNDVWEATKLDRENYFFVDVQSKGKYPNY
                     ALKYMEQKGITPEMEPGDIELLNKYTVDFISFSYYNSRCVRTDENADDMAEGNIFTSA
                     KNPYLSYSQWGWPIDPLGLRITLNHVYDRYEKPLFVVENGLGAKDIADENGYVEDDYR
                     IDYIREHIKAMHDAVSIDGVDLLGYTTWAPIDLVSAGTGEMEKRYGFIYVDRDNSGNG
                     SLRRMKKKSFEWYKKVIASNGEDLY"
     gene            18936..19712
                     /locus_tag="PC414_002385"
     CDS             18936..19712
                     /locus_tag="PC414_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2464775.1"
                     /translation="MKYQHIIEVDKELCIGCGLCKNDCPVNNIIIENKKSVIKKQDCL
                     MCGHCAAICPTKAITLTGFDEPPIELTNKPKLDSDELLMAIKSRRSIRKFKDKEVSSE
                     IIKQIIEAGRYTPSAKNSQDVSYIVLDNKKSIYENEAVKFFRKIKPIANIAIKYSKEV
                     EIDDNFFFKHAPIAIMIITKDKISGSLAASNMALMAESYGLGVLFSGYFSDVANNSPK
                     LKKLLGLKRSNHVLTTLVIGYPDVKYRRTAQKEVATVRYL"
     gene            19728..20093
                     /locus_tag="PC414_002386"
     CDS             19728..20093
                     /locus_tag="PC414_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF2464776.1"
                     /translation="MEKECQQRMQEITSKFKECRKAISAMGDETRQLILIALLESDFN
                     GIRVGEITKKTHLSRPAVSHHLKILKEAEIVNLRREGTKNYYYLDSKKSQWRSLTELI
                     NLIFVGIQHISENDIRKGD"
     gene            complement(20269..21018)
                     /locus_tag="PC414_002387"
     CDS             complement(20269..21018)
                     /locus_tag="PC414_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440038.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HDF2464777.1"
                     /translation="MILYFSGTGNSRYVARKIAQELNDELISLNQLIKEEKTDELIST
                     DKPFIFVCPTYAWRLPIVVTDFIKETKFLGSNRVYFVMTCGGDTAKSINYIQKLCKYK
                     EWQLKGMAEIKMPENYIALFSTTDKETSKQMIKEADKQIYRIISDIRNENEFEIITPS
                     GLGGTIKSGIINTVFYKTIISAKGFHYTDKCIGCGKCVELCPLNNINLKNKKPVWKNN
                     CTHCMACICGCPTEAIEYKNKTQNRERYYLE"
     gene            21496..21921
                     /locus_tag="PC414_002388"
     CDS             21496..21921
                     /locus_tag="PC414_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2464778.1"
                     /translation="MINENLKSLRKINQYTQEELAEKLNVSRQSIAKWESGESIPDIG
                     SCIKLAKLYNVKLDDLVNHSEEKTGIIVPPKGKFFFGAVVVGERGQIVIPKEAREVFN
                     INAGDKLLVLGDEERGLGIVHQRDLINFIGHVGVVHKEE"
     gene            21953..23140
                     /locus_tag="PC414_002389"
     CDS             21953..23140
                     /locus_tag="PC414_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HDF2464779.1"
                     /translation="MSKIVFFSIPAYGHTNPTIEVVRELVDRGNEVLYYSFNEFKDKI
                     EGAGAKFICCDKYLPELLPGDEKKIGKDFPGLIEMIVDTTISLDEKVCRELKDFNPDC
                     IVSDSLSFWGKLFAQKLNIPYVCSTTTFAFNKHTAKLMKQNFIEIIRMIFGVRRINKK
                     IKLLQRKGYEVKNFISIVSNDNDTDTIVYTSKEFQPMVETFSSKYSFVGPSVSKLIIE
                     PKERKRKLIYISLGTINNKNVSFYKNCINAFKDSDVDVIMSVGRSTDIKSLGNIPNNF
                     EVKNSVEQIAILQKTDVFVTHSGMNSVNESLYYGVPMVLFPQHSEQRMVAKRVVHLGA
                     GIMLKEDKSESIKKAVFQVINDNEYKENATKLSKSFYNAGGSKKAADVILQIICNSRQ
                     VDI"
     gene            23332..23736
                     /locus_tag="PC414_002390"
     CDS             23332..23736
                     /locus_tag="PC414_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HDF2464780.1"
                     /translation="MKKYKVIFIVLIIGIISVTGFVVYKNCIKPWKDAETYIDKYMVK
                     QDISKDNIKSITKEKAKKASYEGILYKVYYKDDPRYEYQYFYSDNYYALYVNKVMLQI
                     YDMENNNKLLRSNEELKSVKYPPIYLKYKPDK"
BASE COUNT         9502 a         2466 c         4176 g         7656 t
ORIGIN      
        1 aggtcagatt ataattgttt taagataaaa tgtaataaaa tttaatggag aaattttata
       61 ggtagataaa ttttaatatt tataagatta tgttaaggaa acacttaaaa attagattaa
      121 aattgtgggt atttaaatca actaaaagat attgactatt tagtcaatat ctttttttat
      181 taaaaaggaa tatacatata aataggttag attaaaaatt gggataaata tgatttatat
      241 agcatcattt aattaaaagg agctatatta gtattcattc atacaattgg ataatataca
      301 atgtcagcct tgctttatgg tgtttgtaat aaatctattt cagtttctat tgtaattaat
      361 atgcaagaat ttaagatagg agtatctatg gcttatggtg tattagtaat tggtatatgc
      421 tatatagcat taggaataat ttttaaatta gatagaaaaa gatttacata agataacaaa
      481 ggctaattta taaattggtg cttagaaaac caaatcactt tattagctag agatgaattt
      541 ggagggtgtc actttggtgt tttatgtaaa gtttatggaa gtaaaacatt agataatcgt
      601 gagtaagatt tctataaatc gtatatacta ttggaatcca agaaaaggta ttggccaaat
      661 agctaatatc tttttaattt ttttgactct aatatagcat taactattgc atgagtatct
      721 ttaaatttat agttaatgta tcttaattgt ttattataaa atctaggaag gctaaagcat
      781 aaaacactta aaaagagaag atgtacatga gaaatataat taactatgat gcagacttct
      841 aaaaattatt tagtgaaaag gaacttagag tgtataataa aagttccttt tcactaagga
      901 aaacgattgt tattttctga cattcattac cctgatatta tttaaatata aacaacaaca
      961 aaattaacaa gaagcaaatt caacctaaaa tttaaaatat cgggaggtac aatatgttaa
     1021 taaacatgaa ggaaatgcta aaagtggcac aagaaaatca atttgctgta ccagcattta
     1081 atataggaag tgggcaaata cttaaagcag tagtacagag tgctaacgaa aaaaatgcac
     1141 cagtaatact tgcaatacac ccaaatgagc taagttttct aggagatagc tttgtagcta
     1201 gttgcataga agaagcaaat aagagtaaag tacctatggt aatacactta gatcatgggg
     1261 aaaataaaga acaaatatta agggcaataa gatgtggatt tacatcagta atgattgatg
     1321 gctcacattt accatatgaa gagaatgtag ctatatctag ggaaatagta gaaatagcaa
     1381 aaggattaaa tgtgtccgta gaaggagaac ttggaacaat aggaacaaca ggaacatcaa
     1441 gtgaaggtgg tactgatgaa ataatatata cagattcaaa attagctaag gattttgttg
     1501 aaaaaacagg agtagacaca ctagctattg caattggtac agcacatggt atatatccaa
     1561 aaggttttaa accagagctt aaactagatt tattaaaaga aataagagaa gtagtagata
     1621 tacctttagt tcttcatggt ggttcatcaa atccagatga agaaatagca caagcagtta
     1681 aactaggagt ttgtaaaata aatatttcga gtgatgttaa gtcggcatat tataagaagt
     1741 gcagagaact attagaacaa aacccaagct tatatgagcc agatactata tatccaccat
     1801 gtataaaagc tgcaagagaa gttatagagt ttaaaatgaa tctattcaat gctatagata
     1861 agttaaagtg cttttataaa aataaaaatt aattatgagg taacaaatta aaaggagggg
     1921 agaatttatg ataggtaaaa tactacgaag cgattgtatt gtactagata aacaattaca
     1981 aacaaaaaaa gatgtgatta attatatggc aaatcatttg taccatggaa attatataaa
     2041 taataaagaa aagtttatag ctgatattaa taaacgtgaa aaagaatcat caactaatat
     2101 gggtaattta atagctatac ctcatgctaa gtcaaatgta gtaaatcaac ctgtaatgat
     2161 gtatttaaga acagatagag agattacaga taatgatggt caaaaactta ggtcattttt
     2221 tatgatagca gttccagaat caggttcaga tgaacatctt aagattatat ctaaactagc
     2281 tacatatcta atggaagaaa attttcttaa gcatctacta agtgcagaaa ctccatacga
     2341 aatattaagt atatttaata ataaagctgc agaatcagga gattttagta ataatcaaaa
     2401 taaaggtctt atagttggga taacaggatg tccaacaggt atagctcata cttttatggc
     2461 aaaaaaagca ttagaggaag cagggcaaga acttggatat gaagttaaaa tagaaactca
     2521 tggagctata ggtgtagaaa ataagttgac taaagaagat ataaaaagag ctgaatacgt
     2581 aataatagct tgtgataaag aagttcaact gagtagattt ataggaaaga aagtgctaca
     2641 aaagaaagta gtagatgcta agaatgtttc attagctaaa aatttaatta taaacgctga
     2701 aaatggaatg gctaaagaaa ttaaaccagg tggaaagact ttagaagatt caaaagaaat
     2761 gcttcaaggg tcagagaaaa agggtgttta taagcactta atgagtggtg tatctgaaat
     2821 gattccattt gtagtgatag gtggaattgg tatagccata gcttttatgt ttggtatatt
     2881 tgcatcagac ccaactcatg aaacatacaa ccctatagct ggattctttg gacaacttgg
     2941 aggcgattca gcatttaagt tatttatacc gatacttgct ggttttatag ctaaatcaat
     3001 agcaggaaga caaggatttg ctcctgctat gatagctggt tttatggcag ttattggagg
     3061 ttcaggtttt ataggtggta tggtagctgg ttttgcaaca ggatatatag ctttaggagt
     3121 aatgaagata acagaaaatt taccagatgt attaaaagga gtaaatgctg tattgatatg
     3181 tccacttttg attgcattta cgagtggagc tataatgttc tttgtagtta atacgccagc
     3241 atcatggtta aatgaagctt tacaatcttg gttacaaagt atgtctggaa ctaataaagt
     3301 attattaggt gcaatattag cagggatgat ggcatcagat atgggaggac ctattaataa
     3361 aacagcttca gcttttggat tggctatgtt ttcaagtaat atatttgaac catcagcagc
     3421 attaatggta ggaggaatgg tacctccatt agcaatatca ttagctatgt taatattcaa
     3481 aaataagttt actaaagaag aaagaaagtc atctctacca actgctataa tgggggcatc
     3541 tttcataaca gaaggcgcaa taccgtttgc agctgcagac ccattaagaa tgattccagc
     3601 aaatataatt ggttcttgta taggtggggc aatatgtatg agtttagata taactttaat
     3661 ggcaccacat gggggaatat ttgtaatacc atttgcatgt agtgcaccag ttgcatatat
     3721 agcttgtata ttgattggta cagtaataac agcattgatt ataggggtaa caaagcctac
     3781 tataaggagt gaaaatagtg aagttgaatt acttggtgct taaattataa ataatagtaa
     3841 aaataattaa tggaataaca acaagtatat agaataataa taaggatatt tagaatcagt
     3901 taactatttc taaatatcct taatttttat tttgaaatta tattatctat tatatcaatt
     3961 tagtaaggtg tatcataaat tcttttatga tacagaagta tcttagtaat tttacatagt
     4021 tagtaaatat gttaaaatag tacttaatat aaacatagaa atatataaac ataggtaggg
     4081 agggctaagt atgcttgtgt cgaggcaaat tgaatttctt aaattgatac taaacgaaaa
     4141 taaatataga cctataaaat actttaaaga taaattaaat gtatctgata aaactttaca
     4201 aaaagatttg aagatgattg agaaatattt agaaactttt aatataaaaa ttgatataaa
     4261 aagaggctat ggaatattaa tagaagattt agcaaagaag aacatagact taattaatag
     4321 tttaaatata caatcgaagg aaaataaatc tttatcagta gagcagagaa gaatggagat
     4381 tttaaagtat ttattgctaa attcaaataa tataacttca ataaatcagt tagcagataa
     4441 atattatgta agtaaaactt ctatagtaaa tgattttaaa tatatagagg catggataaa
     4501 agaatacaat ttaaaactta ataaaacttt agaaggaact aagattatag gtaaagaagt
     4561 ggatataagg aaatcgattg ccaaaatgat ggatgattta ttagatgaaa attatgaaca
     4621 acatgatata tctgagttaa caagattaga ttctgctaca tttagtgcat tgataaattt
     4681 atttgatatt gattctatta tatttgtaga aactataatt acagaattag aatacaattt
     4741 aggatatact ataaatcagc cctactatat aaatttaatt acccatatac ttatatgttt
     4801 aaagagaatc gaagaaggta atcaaataga atctcaggaa gaaagagaaa tttctgtaga
     4861 taatttggac gagttagtat ataaaaatgt cactacactt atagataaga tagaaaatag
     4921 atacagagta aaaataacga aagatgaaat tatgtacata tatatttttt tggtatcatc
     4981 tggatttagt agcgaaaata atagtagtta caatgatgaa tcagatagat taagtgaaag
     5041 tgaaaaagtt tcatctataa tgatagagaa tatgtcacga tttttaaata taaatctcaa
     5101 aaatgatgac ttactacaga aaagtctagc atctcatgta aggcctatgt taaacagatt
     5161 aagatatgat atccaaataa aaaacccgtt gttaggagag atacaagagc gattttcaga
     5221 tgtgctggga ttatgtatga tgactataaa tattatgaca gagtatgata atctgaagaa
     5281 tattagtata gatgaagttt cgtatcttgc cacatatttt caagcggcaa tagaaagaaa
     5341 tatgagtagt aaaagagtaa tagtagtttg tcatacagga tatggaacat ctcagctcct
     5401 tgcagccaga ttaaaaagag aatttcctga atggactata gttgatatag ttcctatgca
     5461 tcagttgagt aagcgaaatt tagatgatgt agactttgta atatctactg taaacttaga
     5521 tttgaaagat aaattacata tagttgtatc agtactatta atggaaagtg atattaataa
     5581 tataaaaaat gctttacttt ctaaacctaa aaaaagtatg aacttagata caaacttatt
     5641 aggaatgtat ttagaagata atatatattt taattttgat gtagctcaga tggaaagttt
     5701 aataggtgtg aatttaggga gtaaatatga atctatttca ttaggaaaag atttaaatat
     5761 atatatacat aagcaaagta aattaaataa agggataatg aatatcaata atgttactag
     5821 aacgattgat atttatttag aaattagtaa tcaaagtttt ttaaagaata ttttaatgga
     5881 aatatcaaat ttatataggc aaaagaacta tataaactat ataatagatt gcaagagcaa
     5941 agaggatatt aaaatattat ttaagaataa tctattggag aaaacaaatg gaaattagag
     6001 aaatattaac agaagaaagc attattttag atttgaaatc aactacaaaa gaaggcataa
     6061 tacaggaatt agtagataaa ttatacgata caggaagtat aactttaaaa agtaagttta
     6121 taaaggcatt aaaacataga gaaaaaatag gctctactag tataggttat caaatggcca
     6181 tgccacatgg aaaaacaaaa tatgtaaaga aggcatccat agtaataggt aaatctaaaa
     6241 aaggtgtaga ttggccaagt atagatggct tacctgtaaa ggtaataatt atgtttgcta
     6301 tgccacaatc tggaggtaat gaagatatga tattaatatc taaaatagct tacattttag
     6361 gtggtaaaaa tataggtgaa agacttttaa atgcacatac aaaaaaagaa atattagata
     6421 tattttatga agtgtaggta ttgtataaat tataaatttg aggtttttaa aataaaatgt
     6481 gtattctttt aaatagtata gattaatttt tagacactag agaggagagg atatatatgg
     6541 caatatattt agaaatgcct aaattaatac atgaatttcc ttttcgtact ctatcaaatg
     6601 aaggagaagt attaacaaca ccacactggc ataaggaaat agaaatatta cttataacag
     6661 aaggtgtagt aaatctattt ataaatgaca agcctatgca attaaaaaaa ggcgagatag
     6721 tgataataaa aggaggagat attcattata tactgccatc accgggaagt gaaagattag
     6781 tatttcaatt tgatatatct ttttttaatg atttaatttt attaaatgag gagaaagaaa
     6841 gcttaattaa tttattcagt agtataaaaa aatgtagtag agattggtca gcaaaaataa
     6901 gaaagtcagt tttcgatata ttgatagata tctacaatga agacctatac aaaatagaag
     6961 gatatagtta tgcaataaaa ggtaaaatgt ataatttaat tcctatatta tatagacaga
     7021 ttcctagaga aattgaagaa aatacagtag aatatgaaat aaactctaaa gagatattag
     7081 agagattgaa caatatattc aaatatgtag aaaaaaatta taaacaacct ataaaattag
     7141 aagatgtgtc ttatgaagta ggatttagtg tatattattt tactaaattt ttcaaaaaaa
     7201 atacaggaaa aacatttata acttttttaa atgaatatag attagaaaaa gcaaagtgga
     7261 tattacttaa taacaatata tccattgtag atttggttga agaagttggt tttggaagta
     7321 caaaaacttt ttacagagta tttaaagaaa aaattggagt ctctccatta gaatttaaaa
     7381 aaagatataa tagcttggag aaaaaaggga gtgtctcaaa atgaacttag ttcatgaggc
     7441 actttctttt atatgaaatc aaatatattt tcagtatttc aacaatgaaa attccattca
     7501 tttataaagt ctaaatttta gttctcaata caacctctat ttcgaaataa atccttttat
     7561 atatataaat atacagcaat atatgagaat aagataacaa taatgaggat ataaaaatgt
     7621 atttgctata tatataatta tatacataga tatctaatat tgatatttaa tatgaaagta
     7681 ttgaaaatag acaatattaa ttatagtgag taaaaaatat aattggattt ttaaattgaa
     7741 ttatttaaaa ggtatagtat gaaaaaaatc ttataactaa atatgagata gaaattataa
     7801 aattttatac tgtgaaagag gtagatgcat gaaattaggg aaagattttt tatggggagg
     7861 ggcaatttca gccaatcaaa gtgaaggatt ttatttagat ggaggaaaag gtttatctca
     7921 gtttgataaa ctcccattaa atgataagag attaaaaaat gtatgtttag atgaggataa
     7981 tattttaaat gaaaataatc aatactatcc aagtcatata gggattaaat tttgtgaaaa
     8041 ttacaaagaa gatatagcaa tgttagcaga aataggtttt aaatgtttta gattttctat
     8101 atcttggtca agaatattcc caacaggaga agaaagtgaa ccaaatgaaa agggattaca
     8161 actttatgat aatataataa aagaattaaa aaaatataat atagagccat taataacgat
     8221 aagccacttt gatatacctt attctctagt tgaaaaatat tctgggtggg ctgatagaag
     8281 agtggttaat ttatatgtaa aatatgctga aattttgatg aaaagatata aagatgatgt
     8341 caaatactgg attcctttta acgaaatgaa tatgataatg catattccat atataggtgc
     8401 tgggttaact tttatagctg atgaaaatca acttcagaaa aaatatcaag cagcacacca
     8461 tcaattatta gctaatgcaa aaactataga aataggaagg tatataaata aagaatttaa
     8521 gtttgggtgt atgttagctg ctggaaaaac atatccatac acatgtaaac cagaagatgt
     8581 atttgcaact ttagagcatg agaggcataa tttgtttttt tctgatgttc aagttagggg
     8641 tgtgtatcca agctttatga gttattattt aaaatcaaat caaataaaat taataataaa
     8701 agaggaagat ttatcaacac ttaaggataa tactgtagat tttgtatctt ttagctatta
     8761 ttctagtgct tgtagttcag ctagagctga aggtttagaa aaatcaaagg caaacggacc
     8821 agaaaccata aaaaatccat atcttccaaa atcaaatagt gtatggcaaa acgaccctgt
     8881 agggttaaga ataactatga atcagttata tgaaaggtat cagttacctt tatttatagt
     8941 agaaaatgga ctgggtactc aagatattgt aaatgaagat ggaaaaataa ttgatgatta
     9001 tcgtatagat tacatgaaag aacatattaa aaatatgatt cttggagtaa atgaagatgg
     9061 aatagagctt ataggatatc ttatgtgggg atgtatagat gtaaccagtg taagtgaagg
     9121 aaagatgaca aaacgatatg gcataattca tgttgattct gatgactatg gaagaggctc
     9181 atttaaaaga actaaaaaac aaagtgcttt ttggtataaa aaagtaatag aaacagatgg
     9241 agaaatttta tttaatgata tggaaactca tgcataattc attttaagaa aggagtgtta
     9301 ttatgagttt aatatttact actaaggacc aacttgtaga gctagggttt agtaaggata
     9361 caataaaatc caatgaaacg ttgtttacac tagctaatgg acatttagga gttagaggga
     9421 atattgaaga gtctgatttt aataatgagt atgtagataa tatgggtaca tatgtaaatg
     9481 gattttatga agaatctcca attacatatg gagaaagcgc ttatggctat gcaaaacaaa
     9541 atcagactat ctgtaagtta cctaatgctc atatagtaaa tttctctatt gaaggagatt
     9601 gctttgattt atcaaaaggt attacaagtg aacatactag gatactagat ttaaatgaag
     9661 ggattcttaa acgttcattt atatgggaaa actctcgtgg tagaaaagtt aaagtatcta
     9721 tagaaagatt agtatcacaa gatatagaag aattaatgat gatttcatat acaataacac
     9781 cattgaattt taatggaaaa ataaaattta aaaataaaat ggaaaatagt atggagaagt
     9841 ttaattgtga aaaagtagat gacccaagag tggcatctat aaataagaag caatttaaaa
     9901 ttaatgtaaa aaaatataac aataagtatt ttatgaattt agttacagaa agaagtagat
     9961 tagggttatg gtgtgggttt gattcatata ttacttgtgg aaatattaca aatgagacct
    10021 ataatgtttc agatgaatgt attgagcgag aaatagaagt ctatgccaaa aaagatgaaa
    10081 ggttaaattt agaaattgtt gtaggctata gtagagtata ttctgattta aatctaaatg
    10141 aagaacgttt aaaatcatta gaatgtctgg agtcgttaaa atgtttagaa tcagaagcac
    10201 atttaaaatc attagaattg gttttaagag aagcatgtaa tattggattt gataaagtca
    10261 aaaatattca gagaaagaaa atgaatgagt tttggacaac ctctgatgta gagattgaag
    10321 gcgataaaac tcttcaactg gggataaaaa ccaatttatt tcatatttat caatcagcag
    10381 gaagagatgg aaagactaat atatcagcaa aaggtcttac aggtgatggc tatgaggggc
    10441 attatttctg ggatacagaa atgtatatat taccattttt tatatatact caaccatcca
    10501 ttgctagagc tttattagat tatagatact caatcttaga tgcatcaaga gaaagagcta
    10561 gagaacttgg aataaataaa ggttgcttat attcatggag aactataaat ggtaatgaat
    10621 gcagtgcata ttatccagca ggaacagcac agtttcatat aaatgcagat atagcttatg
    10681 gtgttagaac gtattttgaa gcaactaatg atgaagaatt tatgatgcaa aatggcttag
    10741 aaattcttat agaaacatca agattttggg ttgaatttgg agattatata gaatcaaaaa
    10801 ataataaatt ctgtataaat ggagtaacag gaccagatga atatacagca atagtaaaca
    10861 ataattacta cactaattta atggctaagt ataatttaat atatgcagta cagatggtag
    10921 ataaatttaa gcataattta ttttcaaatg aagtatttag aaaagttaaa ttacaagaat
    10981 atgaagtgaa aaactggata aatgccgctc aaaatatgta tttaccatat gatgaagaga
    11041 agaaaataag caaacaagac gattcatttt tagaaaaatc agtatgggat tttaaaaata
    11101 caccaaaaga aaaatatcca ttacttctcc attatcatcc attaacaata tatagatatc
    11161 aagtaaataa acaagcagat actgtacttt cagcgttgct tttccctgat gagtttagct
    11221 tagaacaaaa gaagagagat tttgaatact atgaaaaaat aactactcat gattcatctt
    11281 tgtcaaggtc aatatttgga atgatggcaa gtgaaattgg aaatcatgaa aaagcttata
    11341 actattttat ggacacagca ttaatggata ttaataacct acaaaaaaat acaagtgatg
    11401 gaatacatgc tgctaatatg ggtggaacat ggatgagttt agtatatgga tttgcaggaa
    11461 tgaaaataaa aaatgataaa ttaagttttg aaccaaggat acctaaacac tggaaaaata
    11521 ttaaatttaa tatattattt agagataatt tagtttttgt aaatatagaa gaatataaat
    11581 cttattataa gttaatcaaa ggtagaaata taaccatatt acatgataat aaagaaataa
    11641 aattatcaaa gaacataata gaagttaata attgttttaa agggagtaat taatatgaaa
    11701 gcagtcttat ttgatcttga tggagttata acagatactg ctaagtatca ttttgaagct
    11761 tggcatgaga tagcaaaatc gctagatata gaaatagatg aaacttttaa tgaaaaatta
    11821 aaaggcataa acagggaaga atcattagat aaaatattgt tatctgctaa taaagtagat
    11881 agttatacta aagaggaaaa gttaaaggtg tgtgaagaaa aaaataacat ttatataaag
    11941 ttaataaact cattaacata taaagatata ttaccaggga taaatgagct tataaaagaa
    12001 cttaaaaaaa ataatgtgaa attgggaata gcatctgcta gtaagaatgc acctaggata
    12061 ttagaatctt tgggagtaaa agatgaattt gattgtatag taaatccatc tttattaaaa
    12121 aggggaaaac catatcctga tatttttatt gaagggagca agatgctaaa tttaaaacaa
    12181 tatgattgtg taggaataga agattcttat tcgggtatta aagctataaa tgctgccaat
    12241 atgacatcaa ttggtgtggg gaaaaaagaa gtactaggaa aatgtgacca tattgtagaa
    12301 gatactagct tattaaatta caatacacta ttacaagctt ggagtaaaaa gattaatttg
    12361 gattaaaatt atattaaaaa ttattttaaa agagaggata aggtatgaag aataaatatg
    12421 aagatgtagc tcaattgata attgatggac ttggaggaaa agataatata attgacttaa
    12481 ctcattgtat gacacgatta aggttcatct taaaagatga aaagaaagtc aatgcagata
    12541 aattaaaatc tatagataag gtagttggag taaactcaac ttcaactcaa tatcaggtca
    12601 taataggtaa tgaggtaggt gttgtattta atgcaataat aaataagggg ataaatacca
    12661 caggagatat taaaagcgat ataaaaaata aaaataaaaa aaatagtatt ttaaataata
    12721 taatagatac aataactggt tgtatgacac ctatgatacc agcccttaca gcatctggga
    12781 tgataaaagt tatattatct ctttgcacca cttttaattg gctatcatca aattcaagta
    12841 catatagagt attagatata ataggagatg tagcatttta ctttatgcct ataatacttg
    12901 caataaatgc agctaaaaaa tttaatgtta atacatcaat agcagttata gtagtaggtg
    12961 tgtttttaca tccaaatttc tctgcatggg tatcaagtgg ggaccctata tcatttatag
    13021 gagtaccaat tcaaggtgtt atatatgcag catctgttat ccctgcactt ttaacagtat
    13081 ggatgatgtc atatatagaa aatttcattg ataaattaac acctagcatg ttaaaaacaa
    13141 ttttaaatcc aactttagta ttattaataa gtgcgccaat agcattaata gtaattggac
    13201 caataggaaa tttcttagga gagggattag ctagtataat aaatttacta caaggaaggt
    13261 taggatttat aatggtttgt ttattagcag ctgcaatgcc atttatcgtt atgacaggga
    13321 tgcatcatgc attgactcct atattcatat cagcatttgc aactacagga caagaatcgc
    13381 taatattaat atctcaagtt tgtgctaatt tagcgcaagg tggcgcaaca ttagctgtag
    13441 cattaagaag caaagatagt aagatgaaac agttagcatc agctgcatgg atatcagcta
    13501 ttatgggtat aactgaacca gctctttatg gagttacatt gaaacttaaa aaacctgcta
    13561 tgttagcatc tataggagca ggaatcgcag ggttatttgc aggaattact catgtgactt
    13621 tatatgttcc acaaaatagt ataatggcag tacttggatt atcaggggaa aaggggattt
    13681 caaatatagt aaatggactt atagttatat ttatagcatt ggttgctcca tttattatga
    13741 catatgtttt tggagttgaa aaaaatgtta aataaagtac agataatgta gtagaggaag
    13801 tataatatta actatttaca ttaatagaat ttttattatt attgactata acttgttttg
    13861 tatttatatt gatgcaaaga aataagttaa tattaataag aataattatt ataactgaca
    13921 aaaagcaaca taaaagattg atgaagtata tggaaataaa aagttgacaa aatatgtctg
    13981 atgttttatc atattaattg gaattaggta gtaattgttt aagtacaagc taagcttatt
    14041 cattaataat agcagtatcc taggaaggag tatgcccatt attatggata agcttttttt
    14101 atgcttaaaa ggaggataat catgataatt aataagatat taaacaataa tgttgtaatc
    14161 acattagatg ataatgatga agaagttatc gttatgggta aaggaattgg ttatcaaaaa
    14221 tcaaaaggca atctaataga taaaactaaa gtaaataaag tatttagaat atcaaataaa
    14281 gaaatatcaa acaaactaca agaattactc aataatattc ctatagaaca catgaaatta
    14341 tcaagtgaaa ttatagaata cgcacaaata aagttaaaca agaagctaaa tgaaagtatt
    14401 tacatatcct taagtgacca tacatattct gcaattcaaa gaatgaaaga aggaataaat
    14461 gtaaaaaatg caatcctttg ggaaattaaa agattttata aagaagagtt tgaaattgga
    14521 atgaaagcac tagatattat tgaaaacaaa acaggtataa aattaccaga agatgaggct
    14581 ggatttatag cctttcatat agtgaatgct cagttaagtg aaggtcatac tttggcatct
    14641 gatattacga agttaataca agaagtactt agcatagtta ggtatcattt taggatagaa
    14701 tttcatgaag aatcagtgtt ttattataga tttataatgc atttaaagtt ttttgcgcaa
    14761 agattgcttt tagatagtgc acatgaaggt gagacagaca aagaattact aagcattatt
    14821 aaatctaaat ataataaaga gtttgaatgt gtagttaaaa ttaaaaattt cattaaaaaa
    14881 caatataact acatattaac agatgatgag cttatatatt taacaataca tttagctaaa
    14941 gtagttaaag actcaaatat gtaagtgaaa taatttttca gcagtatatt aagaatgatt
    15001 gaaaggagtg atactaacaa aattatactt atggttggat ggtaacattg agttggccaa
    15061 accttcaaat ttcaaaaaat taatttaaac gaaatatgga ggagaaaaat gggaaaatac
    15121 aaacaattag cacaagagat agtaaacaat gttggtggaa aagaaaatat tagtggatta
    15181 gtacactgta ttacaagatt acgttttact ttaaaagatg aaagtatagc aaatgacaat
    15241 gtattaaaaa acatggaagg tattgttact gtaatgaaaa gtggaggaca atatcaagta
    15301 gttataggaa atcatgttga agctgtctat aaagatgtag ttgaaataat tggtttagat
    15361 gattcaagta caagttcaga aactaaaaaa tcaggtaata tactagataa aggtatagat
    15421 ataatatcag gaatttttca gccagtgtta ggaataatgg cagcttgtgg tatgttgaaa
    15481 ggatttaatg cattatttgt agcaatggga ttatatagtg atgtatctgg tggttatatg
    15541 gtaataaatg cagcgggaga tgcattattt acattccttc ctttgttctt aggatatact
    15601 tcagctaaga agtttggatt aaaaccaatg ttgggattag cactaggagc tgcagtatgt
    15661 tatcctgcta tacaaggtag cagtatatca gcaggagcag atgtgatgta cactttattt
    15721 aaaggaacaa tgtttgcatc accagtatat attgatttct ttggactacc aattgtatca
    15781 attgattata ctggaacagt cgtaccaatt atatttatag tgtattttgc ttctaggtgt
    15841 gaaaaattat ttaataagtg tgttccaaat ttagttaagt ttttctttgt gccaatgctt
    15901 acattattaa taacagtgcc tgttgcactt atagttatag gccctatagc aacatttggt
    15961 tctacactta tttcacaaat tgtaatttca ataagagatt ttagtccact attggcaggt
    16021 gctatagtag gatttacttg gcaaattctt gttatttttg gtatgcactg gggatttatt
    16081 cctgtatata taaacaatgt tatgacttta ggatatgata atgttatgat gccattcttt
    16141 gcctgtacat ttgcaagttc ggcagttgtg ttggcaatat ttttcaagac taaagataaa
    16201 aaactaaaag agatggcaat accaaacttt atatctggta tatttggagt tacagagcca
    16261 gcaatttatg gaatattatt gcctttaaag aaaccattta taataagttg tattgctagt
    16321 ggtattggag gcgctttcta tggattcttt aatcttagaa aatttataac tggaggaatg
    16381 ggaatatttg aacttccagc aatgattgaa ccagatgggg gaatgggtaa cttaatagta
    16441 gctctttctg gtatagctat atcaatggtc gtagcttttg tattaactat gattttatat
    16501 aaagatgaga aagtagaaga gtctaaaaaa gtaagaaata agggtaaaga agaaataaaa
    16561 gaagtcaagt ctactaaatt agaaagagaa atagtaacaa gtcctataaa aggtgaagta
    16621 ttaaaattaa gtgatataga ggatgcagca tttgcatctg gtgtactagg tcaaggggtg
    16681 gcaataatac ctagtgatgg aaaagtagtt gctccagtag atggggtagt tacaacctta
    16741 tttcctagtc tacatgctat agggattctt tctgatacag gtgttgaagt gcttattcat
    16801 attggattaa atactattca attagaggga agaggttttg aggcatgtat aaaacaaggt
    16861 gatagaatta caaaaggtca aactatatta aactttgatg ttgatgcaat aaaagagttg
    16921 gggtatagta ctgtaactcc aattgttatt acaaattcat cacaattttt agatgtagta
    16981 gaaacagaaa gtaaaaatat tgaattagaa gacaatctaa ttacagtctt attttagtaa
    17041 agattgaggt attagcataa tctagtgctt tggttataat tttaggaggc tttgactatg
    17101 agttttaaga atgatttttt atggggagga gctactgcgg ccaatcaatg tgaaggtgga
    17161 tacaatgaag acgaaagagg attggcaaat gtagatgtat gtccaacagg aaaagataga
    17221 acagcagtta ttacagggaa actaaaaatg tttgattttg atgatgagca ttattatcca
    17281 gctaaaactg gtattgatat gtatcataat tataaagaag atataaagtt atttgctgaa
    17341 atgggattta aagtatatag aatgagcatt gcatggagtc gaatatttcc aaaaggtgat
    17401 gaagaaacac caaatgaaaa gggacttcaa ttttatgaaa atatatttaa ggagtgtaaa
    17461 aaatataaaa tagagccttt agtcactatt acacactttg attgtccaat gcatttagtt
    17521 aaaaagtatg gtgcatggcg taatcgaaaa atgataggtt tctatgagaa attatgcaat
    17581 gttatcttta gaagatataa aggattagtg aagtactggc taactttcaa tgaaataaat
    17641 atgattttac atgctccatt tatgggtgct ggtatttgct ttgaagaagg tgaagatgtt
    17701 gaaaagataa aatatcaagc tgcccatcat gagcttgtag ctagtgcaat tgcaactaaa
    17761 atagcacatg aagttgattc aaataactta attggatgta tgtttgcagc tggaagtgta
    17821 tatccttata gttgtaatcc taatgatgta tgggaagcta caaagttaga tagagaaaat
    17881 tacttctttg tggatgtaca atctaaaggt aaatatccta actatgcgtt aaagtatatg
    17941 gagcaaaaag gtattacacc agaaatggaa cctggtgata ttgaattatt gaataaatat
    18001 acagtagatt ttatttcatt ttcatattac aatagccgtt gtgttagaac cgatgaaaat
    18061 gcagatgata tggctgaagg aaacatattt acatctgcta aaaatccata tctttcatac
    18121 agtcaatggg gatggccaat tgacccatta ggtttgagaa ttacactaaa tcatgtatat
    18181 gatagatatg aaaagccact atttgttgta gaaaatggat taggtgctaa agatatagct
    18241 gatgaaaatg gttatgtaga agatgattac agaattgatt atataagaga acatataaaa
    18301 gctatgcatg atgcagtttc tatagatggg gtcgatttat taggttatac tacatgggca
    18361 cctattgatt tagtaagtgc tggtacaggt gaaatggaaa agcgttatgg atttatatat
    18421 gtagatagag acaatagtgg taatggaagt ctaagaagaa tgaaaaagaa aagttttgaa
    18481 tggtataaga aagtaattgc ttcaaatggt gaagatttat attagacaag aaatctatgt
    18541 taaataagaa atttgtatta aataagaaga gtataaaaat taataataag tataattttt
    18601 aactttttta actatagaaa atcaatacag aattatttta cattgtattt atgcaatagt
    18661 atgattcaca aataaaattt tagaaaataa aattgttaag aagtatggga agtaagtgca
    18721 atttactttg taataaaaga tgaaaaaggc aagcttaggt tttaagcttg tcttttttat
    18781 tatgtataaa ctatgatact ttaaaattct atgaaaaatt tatttaatag agaaattaaa
    18841 aatttaatgg taatcaaatt ctttaccaat gaatataaaa aatgtctacg tgttataata
    18901 gtttaaacta ataaacgaaa ggtagatgaa tgaatatgaa ataccaacat ataatagaag
    18961 ttgacaaaga actttgcatt ggatgtgggc tttgtaaaaa tgattgtcca gtaaacaata
    19021 taattataga aaataaaaag tcagtaatta aaaaacaaga ttgtctaatg tgtggacatt
    19081 gtgcagcaat atgtcccaca aaagctatta ccttgacagg atttgatgaa cctcctatag
    19141 aacttacaaa taagccaaag ctagattcag atgaattact aatggcaatt aaatctagaa
    19201 gaagtatacg taaatttaaa gacaaagaag tttcttcaga aattataaag caaattattg
    19261 aggcaggtag gtatacccct agtgctaaaa attcacaaga tgtatcatat attgttttag
    19321 acaataaaaa gtcaatttat gaaaacgaag cagtgaaatt ttttagaaaa ataaaaccta
    19381 ttgcaaatat agctattaaa tattcaaaag aagttgagat tgacgataac tttttcttta
    19441 agcatgctcc tattgcaata atgataatta caaaagataa gattagtggt tcacttgcag
    19501 catcaaatat ggcattaatg gcagagtctt atggcctagg tgttttattt agtggatatt
    19561 tttcagatgt agcaaataat tcgccaaaat taaaaaagct tttaggtcta aaacgcagta
    19621 atcatgttct tacaacatta gtaattggat acccagatgt taaatataga cgtactgcac
    19681 agaaagaagt agcaactgtt agatacttat aagagagggt ggattttatg gaaaaagaat
    19741 gtcaacaaag aatgcaagaa ataacgtcta aatttaaaga atgtcgcaaa gctatctctg
    19801 ctatgggtga tgagactaga caattgattt taattgctct cttagagagt gattttaatg
    19861 gtatacgagt gggagaaatc acaaaaaaaa cacatctttc tcgtcctgct gtatctcatc
    19921 atcttaagat attaaaagaa gcagagattg ttaatttgcg tagagaagga acaaaaaatt
    19981 attattatct tgattcaaaa aaatcacaat ggaggtcact aacagagcta ataaatctta
    20041 tatttgttgg aatacaacat attagtgaaa atgatataag aaaaggtgat taaattatac
    20101 ttgatttaat acaaaaagat tgatttagga actaaaaaga actaaaaagt tcactttgag
    20161 acactatctt ttgaactata cccatcttag gagacagtta aaatatgtaa aactgtataa
    20221 aacaatgggt atagttcatt ttataatggt ttaaatatta agaatatatt attctaaata
    20281 gtatctttct ctgttttgag ttttattttt atactcaata gcttcagtgg ggcaaccaca
    20341 aatacatgcc atacaatgag tacagttatt tttccaaact ggttttttgt tttttaaatt
    20401 aatattattt aaaggacata gctctacaca ttttccacag ccaatgcatt tatcagtata
    20461 atgaaatcct ttggcactaa taattgtctt ataaaaaact gtatttatta taccactttt
    20521 aattgtacct cctaagccac taggagtaat aatttcaaat tcattttcat ttcgtatatc
    20581 agaaataatc ctatatattt gtttatctgc ttctttaatc atttgttttg atgtttcttt
    20641 gtctgttgta gaaaataaag ctatataatt ctctggcatt ttaatttctg ccataccttt
    20701 taattgccat tccttgtatt tacatagttt ttgaatatag tttatggatt ttgctgtatc
    20761 tccaccacaa gtcattacaa agtaaactct gttgcttcct agaaattttg tttcttttat
    20821 aaaatccgta accactattg gcaatctcca tgcataagta ggacatacaa atatgaatgg
    20881 tttatctgtt gatattaatt catctgtttt ttcctctttt ataagttgat ttagtgatat
    20941 cagttcatca tttaattctt gtgcaatttt acgagccaca tatcggctat taccagttcc
    21001 gctaaaatat aaaatcataa taatatctcc ctaactattt tttattttaa ctcttaattt
    21061 tttatttatt atagtaattt attgatatat tctattcaaa tgtatgtaaa atttagtagt
    21121 taagaaaatt ataacacatt agtaattttt atacttattg gtaaagaatt tgattaccat
    21181 taaatttagc aacaagacat caactggact tgtatatgag aagggataat aatttgctta
    21241 tgaaagatta taaatataaa ggttattttg aaggtgtaga aatatttgat aatgtaaatg
    21301 accttaactt aaatacttta acaacagtta gtagatttgt gtttgttttt ttgtttacga
    21361 taacttaagg aaatatgtta cctgaacaat aaatttatat aataaaagta tataaatata
    21421 tatatgaatg ttaagattag ttgatagaat ttaaataata aatccaatat tataaacata
    21481 ggaggtatga aaaatatgat taatgagaat ttaaaaagtc taagaaaaat caatcagtat
    21541 acacaagaag aattagctga aaaacttaat gtttctagac aatcaatagc aaaatgggaa
    21601 agtggagaat ctattcctga tataggtagt tgcattaaac ttgcaaaatt gtacaatgtt
    21661 aaactagatg acttagttaa tcattcggaa gaaaaaacag gtattatagt acctcctaaa
    21721 ggaaagtttt tctttggagc agttgtagtt ggtgaacgtg gtcagattgt cattccaaaa
    21781 gaagcaagag aagtatttaa tataaatgca ggagataaat tattggtact tggagatgag
    21841 gaaagaggat taggaattgt tcatcaacgt gatttgataa attttatagg tcatgtggga
    21901 gtagtacata aagaagaatg atttaaaaaa attataaaag gaggtatgaa atatgagtaa
    21961 aatagtattt tttagcattc ctgcatatgg tcatactaat cctactattg aagtagttcg
    22021 tgaattagtg gatagaggaa atgaggtatt gtattattct tttaatgaat ttaaagataa
    22081 aattgaaggt gctggagcaa aatttatttg ttgtgataaa taccttccag aattactccc
    22141 tggagatgag aaaaaaattg gtaaggattt tcctggatta attgagatga tagtagatac
    22201 tactatttct ttagatgaaa aagtttgtag agaacttaaa gattttaatc cagattgcat
    22261 tgtatcagat tcattaagtt tttggggaaa gttatttgca caaaaactta atattccata
    22321 tgtatgctct acaacaactt ttgcatttaa taaacataca gcaaagttga tgaaacaaaa
    22381 ctttatagaa attatacgta tgatttttgg tgtaagacgc attaataaaa aaataaagct
    22441 tttacaaaga aaagggtatg aagtaaaaaa ttttatttct atagtttcta atgataatga
    22501 tacggatacc attgtatata catcaaaaga atttcaacca atggtagaaa ccttctctag
    22561 taagtattca tttgtaggtc catcagtatc aaagttgatt attgaaccta aagagagaaa
    22621 aagaaagcta atatatattt cgcttggtac aattaataat aaaaatgtta gcttttataa
    22681 aaactgtata aatgcattta aagattctga tgttgatgtg attatgtctg ttggtagaag
    22741 tactgatatt aaaagtttag gaaatatacc aaataatttt gaggttaaaa atagtgttga
    22801 acagattgct atacttcaaa aaactgatgt gtttgttact cattctggga tgaatagtgt
    22861 taatgaaagt ttatattatg gagtacctat ggtattattt cctcaacatt cggagcagag
    22921 aatggtagct aagagagttg ttcatttggg agcaggaatt atgttaaaag aggataaatc
    22981 agaaagtatt aaaaaagcag tatttcaagt tataaatgac aatgaatata aagaaaatgc
    23041 aactaaacta tcaaaaagct tttataatgc aggaggctca aaaaaagcag cagatgtaat
    23101 attacagatt atatgtaata gtagacaagt tgacatataa tagatttgtt aaaaatatat
    23161 atacatacat gtaatttaat caaagtactg acagtggggt cttttaacct accagttggg
    23221 gcatatatat tgatgctaaa tatgagggat gttatatttt aataaaaaag ataacatcct
    23281 ttttttagtg cattatatcg agttttaaat aataaatgaa ggaggattaa tatgaaaaaa
    23341 tataaagtta tttttatagt attaattata ggtattataa gtgtaacagg gtttgttgta
    23401 tataaaaact gcattaaacc atggaaagat gctgaaacat atattgataa gtatatggta
    23461 aaacaagata tttcaaaaga taatataaaa agtattacaa aagaaaaagc caagaaggca
    23521 agttacgaag gaatactata caaagtgtat tacaaagatg accctcgata tgagtatcaa
    23581 tatttttata gtgataatta ttatgctcta tatgtaaata aagtaatgtt acaaatttat
    23641 gatatggaaa ataataataa acttttaaga agtaatgagg aattaaaaag tgtaaagtat
    23701 ccaccaatat atcttaaata taagccagat aaatgattta tgacaaaata gaataatttt
    23761 tattaaaatt tatttaaaaa tatcactaga gcgtttaaat
//
LOCUS       DAMJVK010000059        23747 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.060, whole genome shotgun sequence.
ACCESSION   DAMJVK010000059 DAMJVK010000000
VERSION     DAMJVK010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23747
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.060"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            128..1042
                     /locus_tag="PC414_002391"
     CDS             128..1042
                     /locus_tag="PC414_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HDF2464781.1"
                     /translation="MEENYQVIGLDIGRGYVKGYSKYNGMVKECLFKSVFGDGRNIDF
                     EKYENPIYIDFESVSYFVGSLAEKESITPIRNSDDSKVSFTMRILVAAALNEIAVADE
                     VKLMMGVPYKSFRKTTLKEVVDTYKGKTFKVKDKIKGGHKEIKISDISIFREGDAALF
                     HTLEGKVNEDKAVGMVSIGFRSTEMSFFEKGFVFNDKLSDTLEAGNQDALTMVQKQLK
                     DRGIIRELNEIDSSNDYDELKKVAYIMASESTAQRISSKWKNIDEMDVYVSGGTALHM
                     TFDNRFKVSKDAQMATAKGLFEVGMEQF"
     gene            1055..1327
                     /locus_tag="PC414_002392"
     CDS             1055..1327
                     /locus_tag="PC414_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464782.1"
                     /translation="MKKSSSFYLEEDIFGEIEKYQNDKNISSRNTALERIILEWKNLQ
                     EENKLLKQCLGNGVYRTNKKTNVTEEKKDIKRENPIIKNIFNNMPD"
     gene            1599..2462
                     /locus_tag="PC414_002393"
     CDS             1599..2462
                     /locus_tag="PC414_002393"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464783.1"
                     /translation="MKRGEVLEIGKRKKSFSPEQRAIINNKKELPYHNFKLGGFVFCT
                     YVKNELLFNEVNISKANISRLIYLATYLNYNTGEENLLVRYSQFKEMIPIDRKNMREI
                     LNLKDRAFRNFLADVKKCELLYESNNKFYINPKYFTKGIPNFENKEYTRIYINTSRFL
                     FEHCTPRQHKQLSYIYQLIPYIHFESNILCLNPHETDISKIKKISLYKICQLLNISTI
                     KQDMNKFKKELLRFYIKVEDDKYYFFKYVIVEGNNKNTDYFIINPCVIWKGNNLNLAK
                     KTIDLCFFNCI"
     gene            2640..2888
                     /locus_tag="PC414_002394"
     CDS             2640..2888
                     /locus_tag="PC414_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464784.1"
                     /translation="MKEKKIELIYLEDLDESEKTGVHKNRYQILNEEYEQMIDTYTEM
                     SKRSSYDIVNERHKEDFKDELWDYDDTDYDDGLQGKEV"
     gene            2891..4333
                     /locus_tag="PC414_002395"
     CDS             2891..4333
                     /locus_tag="PC414_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464785.1"
                     /translation="MIYNNNNFYEEIDGKSAQDYIKNLDYSIDNSKDRIKYIEERLGV
                     KHTWFEESNRNYIDTFYNTLTKRNENIITRVKYQEKKQFNDKFWENIFEQTSDSPLDK
                     DGVYYVTIDGEEVVMDYNRFITWCGYNQINPIEYINVKNPFSNSNGTWEYTHHNTSKI
                     KLILNKDDSIYSTSNIAKTLEIMGSYILSVDDKPKETKIRIYNSKELFNRACQEEALL
                     NKIAPANGGNINSNTSNKDGFLEDNSFAFFQLPKNYKKIKDIKVKPKDIKKYPIIKTY
                     SDTYEWYKAKYKELGHKQLSKEELKLKRMVRKNLNALKNDMTDVKNSIERPIIWKAPL
                     ADAGSPEWDYLDMFDKSHVKELLRIQKGNDLQDDLTCIVMDLNNIISKIEFTTVQKDI
                     LNLYRKDKSLEFISNLMNITPQAINNQINKIVNKIIDAYEKNYEENYYYISVCKGKYK
                     KCSRCGKIKLLSRFDKNGKKGYRSNCKNCN"
     gene            4467..4745
                     /locus_tag="PC414_002396"
     CDS             4467..4745
                     /locus_tag="PC414_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HDF2464786.1"
                     /translation="MKKVDFLKEVAEELGVSQIRVREILDVIEKKRNEVLQDGEELTL
                     MGVKYLTKIQKGKEGEITLKDGTKKPWKTEEKRVPKLKIAKSLKDAII"
     gene            5033..5863
                     /locus_tag="PC414_002397"
     CDS             5033..5863
                     /locus_tag="PC414_002397"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464787.1"
                     /translation="MNGEKLIENKELRDRNIDRVDVLEHVKEILTLANTDFSTVELVS
                     EYYEVDRDTIKKLIQRNRIELELDGLKLYKYNDIAEVLENTKKGLDVTFKIPPRGLIL
                     IPKRAILRIGMLLRDSEVAKEVRTRLLDIVHDAEEQTEIVIEEIRNEQNIKEDMLEAI
                     ISGDHNKLSVLQTELIGLKNKRISHLEDVITNSVTITESKAIINKCVRTIAVKKFNCM
                     FGKAWDEFYRFINYKLGINVRNRKGKGLARFSDEEILKMEKIAKSWLEENDIDFKLVI
                     "
     gene            5882..5955
                     /locus_tag="PC414_002398"
     tRNA            5882..5955
                     /locus_tag="PC414_002398"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5916..5918,aa:Ile,seq:tat)
     gene            5964..6054
                     /locus_tag="PC414_002399"
     tRNA            5964..6054
                     /locus_tag="PC414_002399"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5997..5999,aa:Ser,seq:gct)
     gene            6110..6727
                     /locus_tag="PC414_002400"
     CDS             6110..6727
                     /locus_tag="PC414_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464788.1"
                     /translation="MVKYNVHKALAEKKLLEDKIDRCINNFKIVGTKKGSDKNVYETK
                     TSVEDFNVEVSSKYQQIEDLIYNYNALDKAINISNAITNVQISNKNYTVLEAIKRKNS
                     IELDKSLLRQMVSNYDCMMSEVNRRNEEVQKNTDRMFEEKEKSKDGAELISFYKKQQE
                     WSLVDPLKVREKIEKLRDEIEEFEKEVDFALSTSNALTIIDVDLR"
     gene            7097..7285
                     /locus_tag="PC414_002401"
     CDS             7097..7285
                     /locus_tag="PC414_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464789.1"
                     /translation="MKNNKGTVLEKCEIIKQENNTYKIIEHLKDGDHEVLLSDVLDKY
                     TGDTDLNITIRLSETIVQ"
     gene            7362..8561
                     /locus_tag="PC414_002402"
     CDS             7362..8561
                     /locus_tag="PC414_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464790.1"
                     /translation="MEDILKKKEDESLVDYRIRIRLAKVNKEIDLDWGEIVELLGLEC
                     SPDHCRKVSYGLKEAFDYLNSKIQNNSTQEEIDKINEKILELKKIKVQLSDERSLVNK
                     KIREYSRIDNIIDLFNNKIDDISLHKPFLSDSSYKCYESSNQEAIMLISDIHYGLETI
                     NAFNRYNSKIFKIRIQYLKDKIIEYSKLHKVKRLHVMLLGDLISGYIHNSIRLENREN
                     IVEQIIEVSEILSEFIYELSKEIDKIIIYSVGGNHDRVLPKKDENLDKDNFTLLIDEY
                     IKLRIKNINNVIFQENIYDNDIIVAKICGNTCFGVHGDKDKMSTAIPKLTSLIKLIPD
                     YIFMAHLHNCKEDNYGESEICVNGSFSGTDSYAKNLRLSSHAMQKLMIFNESGRICTY
                     NINLSKL"
     gene            8692..9591
                     /locus_tag="PC414_002403"
     CDS             8692..9591
                     /locus_tag="PC414_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464791.1"
                     /translation="MAISKTKCLNCGEEKYTDRYFWKSHSEIFTLNKRLPVCKECFRA
                     RFLLLNGCYNGELVKALKHICFNFDVYFDEKLAKELADKKNKDELIDEYMKIINRNSK
                     YKGKTSLDNLLKEIYTGNNNDKNIVINDEIKLKWGRGFDDYEYKILERKYKEYKEYYE
                     PESLTERKLFEEICIIELEKDKSREKGDMKAFNDLSKLVSSKMQDAEIKPSQKKKAGD
                     SQDDTFGMKMMIYEKNQPVKDRLKEYEDVDGFEAYVNKHMKKPLAVALGLATGKYSIN
                     DGDKDIKFKDDVRDILEGNKNED"
     gene            9581..11329
                     /locus_tag="PC414_002404"
     CDS             9581..11329
                     /locus_tag="PC414_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464792.1"
                     /translation="MRTKYEKRELSSNDSSRNLLESIGEYWGAFYLANPHRFCMDYFG
                     FNLHLFQQILIYMMMKSDQFVFIASRGLGKSWLLGVFCCVIAVLKPGTCVLIAAKRKK
                     QAKLLITSKILGDLYLKSDTLKREIKSFQVNAQEVSIDFWNGSRIEAVVSNDDARGYR
                     ANVLIVDEYRMVDEGTVNDVLVPFLTNPRQPGYLQNPKYRYMQEENKEIYLSSGWYSQ
                     HWSYKKFMETVKGMLSGEDMFACSIPFTCSLEHGLLTKKRILKEMKKESMSDASFMME
                     YCGVFYNESDDAFFKSSWVNPCRVLESMFYPPSDIEYLENKKKRDKKYHLNKIKGEIR
                     IIGADIALARGVKNDNSIYTLMRMLPNEGTYKRCVVHIEAYNGMEAEKQAIRLKQLFS
                     DFQADYMILDTQGIGTTVWSYIQKANYDSDRDEWYDAYTCFNEDNTVDKSLAKKSLPV
                     VYSMKAYADENHKMAMSLRDVLTNRTLELPISDIEAKEMILEKEMIKADEIDKKAELE
                     AKYIAPYLQTTALVNELINLEYSADGGKIKIKEKSGARKDRYSSLAYTNFLADYLEEK
                     EKRKNRNNQKTVMIYW"
     gene            11346..12824
                     /locus_tag="PC414_002405"
     CDS             11346..12824
                     /locus_tag="PC414_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464793.1"
                     /translation="MGKKNNNKSKSKDNNSTQNQINMLNAQIGKYASVKESTTLATEM
                     YKLRSISRDKLRKALSNPYQISNTDILQDASMILKATSGTYRRVLNLISNMNTFDHIL
                     YPKDISRLKTKEKITKAYMNSASQLEKYNIKFTSAWITEKVLELGEVYLYKIEDNSGM
                     VLQQIPAKFCMITMIENNVMRYAINIKKLTDKNIISFPEEIKNIYKKYKSNLLTREEL
                     IDNTYFQLSDKAVAFNYDLDSVKGVPFFCFIFDDLMELEDMKDLKSTNAVIESIKLIH
                     QKIPFGKNDEPLVDLNLIPIYHNSTKANLPKGTAITTNPLELETHTLSDGKSKINDYV
                     KEAKEFIFDNAGINTALLNSDKINNESILNGIIADSLIPMRIQQMIENWVNFELNKDK
                     KANLFNMKFIGTTHFNKMNLSKQYREDMGYGGSKSLFIASTGFTPLQAINTLQAEKLM
                     GFDEFLIPQQTSHTQSSGRPDKSDIGTDNGNSTQAKGNGEND"
     gene            12827..13018
                     /locus_tag="PC414_002406"
     CDS             12827..13018
                     /locus_tag="PC414_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464794.1"
                     /translation="MSKFIYAFSEDDKKLLMEKGYRFICENKLNNKTLYVFENKSKLI
                     NNFSNEEMKRFIFTSKIRF"
     gene            13025..14458
                     /locus_tag="PC414_002407"
     CDS             13025..14458
                     /locus_tag="PC414_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464795.1"
                     /translation="MKKIKILKLPCNLKHYSDKKKLEELNSNLLPVYIYVMHEGTNPN
                     GTKFYEEAIDKAEPTLKNVPILGYVKINEDGKYDFDGHNVLTQVVQTDEGFILEEYYE
                     ERIIGVIPETNKYEKVEIDGQKYVKCKGYIYKSYSNHAYDIIMDSDEIEISMEIDIND
                     YQLDDSDGFYNIKDYVYHGITCLGSDVKGAMGSNCCLTKFSRKINYKEEISKICSEIY
                     ALEHGEGEKNLPNKNQKLNKNSEGYALAVSNLSTEIRNKLKEYKVETENWYGEKVEVQ
                     AFYYNDLIPEENIAIVEDEINWGYYYGIPYIVNEDAVILDYENKKSYIQTWREKQEGE
                     IVQVFSRQEKLKKEIIEKFTEKQKEISNLKASLEPLQAFKEEKEFELFKSKVDDVAQK
                     FELAEDEIKDIKIKAYNKEITLDEYKKELGYIFALKTLSNKQSEKENFSINDDKNNTI
                     KIPISNNNDSFSEPEEISFIKKYSDKE"
     gene            14473..14970
                     /locus_tag="PC414_002408"
     CDS             14473..14970
                     /locus_tag="PC414_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464796.1"
                     /translation="MKKDKAIIQTDKVKNPDVLTGKYVVPSEDTNPINLENGAVINIG
                     ELEDTEYGRDTHKIYKVTNDTLDWGIVDDPATMYDERLDERDYEVSPGQICRCRRLKK
                     GDEVTISLLHIADKSIAVKDKLQLKSDSFQFEKLPTADAKTPVAEVLELWNYEGQDSV
                     TIKVL"
     gene            15058..16068
                     /locus_tag="PC414_002409"
     CDS             15058..16068
                     /locus_tag="PC414_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464797.1"
                     /translation="MATAKTLKRIAHELYTDTFREYTDRDGNTITYRDAESAIKNKLK
                     EMMGGEKYNYYKFQENKWGFYNLISELISDDINRLNEEVFSPFCDFENFDLGDKKEFT
                     VENTNLFKVANIADGVNSTRRQRLLNKKVPTTAFKLSVAIYEEAERFITGRINWVVFV
                     NRVSDSYHYDIARRIAKTFEGAYSTINAKFQATGNSDKVLLELIAKVEGATGKKPIIY
                     GTPLALSNLEGVQTDLEKEEKRKYGFIQTFRGGYKVLSLPNAYDENAAEGKEWALDNK
                     AIYVIPDGEKIIKLGTEGDVLVIENTDEKERDDQQIEYFMAQKIHLGVVTAAKFGVYK
                     IQ"
     gene            16122..16754
                     /locus_tag="PC414_002410"
     CDS             16122..16754
                     /locus_tag="PC414_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464798.1"
                     /translation="MARAKKENISKTEVKVNTLEKDIKSTLNKKEARLNRLELQRKLK
                     QKKQEIDIEIKNISTWDVDYIDPREKQQIFSLSKVGSKDSTEFIDLDTLYRIVRRSPG
                     FFEEHRLIISDVDSLDVECTPMDIIDFLGLNSLYEYIRNPNEDYLEYFLSDKVDINSF
                     EKILNKNNVELTRRLAERAIDLHKKKKFDSGLKAKLLAKRIGMEDLYLFS"
     gene            16769..17398
                     /locus_tag="PC414_002411"
     CDS             16769..17398
                     /locus_tag="PC414_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464799.1"
                     /translation="MGTPLVKIYKKFLDGINDEEMLLLSNEIIEKMMYSYLEDAIVDF
                     NQCKKDLTIKYVDEKGGEIIPAAQLSYTSNYSNKNAEITLMGKDTKEEYELDKDYTIS
                     FEDEKFIISFVVETTEEIIFKYKYLGEIVSDLDLDEIKILSYGMQIHWLQPKINREEN
                     LKQMLTDSDYNAKSGANMLAKLQAREEQLRTRFNKYQQRYMLKNFEGWN"
     gene            17400..18389
                     /locus_tag="PC414_002412"
     CDS             17400..18389
                     /locus_tag="PC414_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464800.1"
                     /translation="MSYLSDTKKRIGLGCSTPKEKRILQLRLSFKKYLKETPTCIEVP
                     ITDIDEICITEDTKRAIVAINDITNNDKRALDEKNLLVESDLDVDVGCYLFYDNCYWL
                     TIFKEHKEMDTYKHFIIKRCNQFFNYKYKGQMYKIPIAVENLTLYSDGMADNKYTSIS
                     DTKRQLYFGSNPVTKTIDIDTRIMLTGKTVFRVTSINDFEYNGRETGADGLIKAICLQ
                     DALISKDDTINNIAWNDLSENESVIIPFSKIMGDGFINLGEENEYKIDHSQGVEWLLD
                     KQYGYCNIINQDEKKCVIQANTLAKYSGFEALLLAKDKDTNKTIDTKKITLRG"
     gene            18404..18898
                     /locus_tag="PC414_002413"
     CDS             18404..18898
                     /locus_tag="PC414_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464801.1"
                     /translation="MAFPNKMISNIGATLMSNQDFAKFMIYNDESQKDILSMPDIKNP
                     VKELRNKKVFLNRRVEKVLKEADISVFIIMSEYRPCFEGSRTIKKTKIEIGVVCHDEC
                     QSTANGLRDVALVCCIVDIVTQNEEIAGIGKIKLENVYQMYNLNTDYNGFVITVSAES
                     FGDM"
     gene            18898..19674
                     /locus_tag="PC414_002414"
     CDS             18898..19674
                     /locus_tag="PC414_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464802.1"
                     /translation="MLENYYITGLPIKLGETLGTVYQPTIEELIKFDMANLEIVNPFL
                     VLEKSYSQLCNEKIFELKCKYDAIPILDSSEEIELLSDKIKKSLSILYKTDIKNIEYM
                     NNIKIGILIKFDDKKKNAFISREDFGLVSDLILEMFCIDKKNLFKDDEDKWIENTGSE
                     REKQLIAHFKEKERKKREKEAYHLCDYINVVQNIDGYVPIDVILKMTYWQLINAYKTK
                     IQFKNYDESLGFAMSFKYQVEADKIKHWSKEIKIQTSTVK"
     gene            19714..20532
                     /locus_tag="PC414_002415"
     CDS             19714..20532
                     /locus_tag="PC414_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464803.1"
                     /translation="MMRFAIKDASNIIVKNKTTGEPLFYTADLNAFNFKLDSESVYAK
                     AKGANTIAFDGAITASLALEQEVIQMPQLAMLLASDMVEESAKVGKRKLLTSDGTKKV
                     TLENVKPVTNSISAYSVEKDGISLVKKLQFTSTVTGSNTEITISTADFNAGDKVAVFY
                     LEELPKAKVIKIKEESTAPNYVIEADVMAKTADGEYMVLYMTIPNAKAQRSIELNLTA
                     ENPSGFNMTLDVLPDENKEYVVFAFVGDEDVSPVRMASMLGAELIDEKDVKSKK"
     gene            20629..21414
                     /locus_tag="PC414_002416"
     CDS             20629..21414
                     /locus_tag="PC414_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464804.1"
                     /translation="MVFLDDKFLFDNISSESMNIKLVTLGDDEILNEYGLPYEEAIKS
                     DSTFNKNPCYSEDETTIEPITLQFCLCSEHGEAYEWDDYTLENICNWFWQREFKPFIS
                     YDNIEEIYYLKARKIIKRYTKDKKGVLEIEFQPYTNYAYRSFQKVITVKDTREIKLNN
                     VSNVDEEYAPVIDIECLKEGDITIRNSTISDNEEDNLVISGLELNEKITIDNLYYTVL
                     NSSGENRFNIVNRKWIRLRRGVNILKFTGNCKVSIKCKYPIIK"
     gene            21428..21877
                     /locus_tag="PC414_002417"
     CDS             21428..21877
                     /locus_tag="PC414_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464805.1"
                     /translation="MNKIQVDKLIQDEVRAIIPIIDENGKEEYIEVRNPDKETKEEIL
                     NKIWVGMENPDLALSQEDILKMLIDKLTNIELNIEIEDLVDGNISSELENVMYYIGQI
                     ENELTASLLMNTEIKLGQMKNEILQDRVLKETEEIEKMNNIKEKVVN"
     gene            21878..22315
                     /locus_tag="PC414_002418"
     CDS             21878..22315
                     /locus_tag="PC414_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464806.1"
                     /translation="MVFKSLDELVAYTKNKIAASMPEVGKEMKEIVKEEVNKQVYSDY
                     APSLYDRTYELLNAIDVSEITSDSVATEIKDSGSWFDIYSKAHAFPMERFENGGVWGH
                     GSTKYNPVFRPRTNIMEESNSKAQERVPKCFKSKMNELGVPVK"
BASE COUNT        10105 a         2212 c         4170 g         7260 t
ORIGIN      
        1 gtgctaattt taactaataa ctaaaaaatg tcattttata gttgaatttt tgtgcaaatt
       61 taatgtaatt atcaagtttt tatatggtat aatatattta gcaaatattt acttaggagg
      121 aatgaaaatg gaagaaaatt accaagttat aggcttagat ataggaagag gatacgtaaa
      181 aggatatagt aagtacaatg gaatggttaa agaatgttta ttcaaatcag tatttggtga
      241 tggaagaaac atagattttg agaaatatga aaatccaata tacatagatt ttgaaagtgt
      301 aagttacttt gttggttcat tagcagaaaa agaaagtata acacctataa gaaattctga
      361 tgattctaaa gtatctttta caatgagaat attagttgct gctgctctta atgaaatagc
      421 tgtagctgat gaagtaaaat tgatgatggg cgttccatat aaatcattta ggaaaacaac
      481 attaaaagaa gttgtagaca catataaagg taaaacattt aaagtaaaag ataaaataaa
      541 aggtggacat aaagaaataa aaatatctga tatatcaatt tttagagaag gagatgctgc
      601 attatttcat acattagaag gaaaagtaaa tgaagataaa gcggttggca tggtttcaat
      661 aggttttaga agtactgaga tgtctttctt tgaaaaaggt tttgtattta atgataaact
      721 atcagacaca ttagaagctg gaaatcaaga tgctttaaca atggttcaga agcaactaaa
      781 agatagagga ataataagag aacttaatga gatagatagt tctaatgatt atgatgaact
      841 taagaaagtt gcttacatta tggcttctga aagcacagct caaagaatat cttctaaatg
      901 gaagaacata gatgaaatgg atgtatatgt aagtggaggt acagcattac atatgacatt
      961 cgataataga tttaaagttt ctaaagatgc acaaatggct acagctaaag gattatttga
     1021 agtaggtatg gaacaattct aggagtgata ttatatgaaa aaatcatcta gcttttattt
     1081 agaagaagat atatttggtg aaatagaaaa gtatcaaaat gataaaaata taagcagtag
     1141 aaatacagct ttggaaagaa taatattaga gtggaaaaat cttcaagaag aaaataaatt
     1201 attaaaacaa tgtttaggca atggtgtata taggacaaat aaaaagacaa atgttactga
     1261 agaaaaaaaa gatataaaaa gagaaaatcc tattattaag aatatattta ataatatgcc
     1321 agattaaaag aaaatatcta ctcaagaaat tttgggtaga tattaaaaaa aatattgaga
     1381 acatagaggg tatatgatat aatagaaata gaacatagag ggtaatgaaa tttctgaagc
     1441 aacatgttgc gaagaacaat tgcaagatga tttttatctt gtaattgaaa taaaatttat
     1501 aagaaaagag gtagatattt atagttagag gtaatgaatt tataaaagat gttattgtaa
     1561 cagacaaaag aacaggagaa atattggagc ataaagggat gaaaagaggt gaggtacttg
     1621 aaattggaaa gagaaaaaag agtttttctc cagagcaaag ggctattatc aataataaaa
     1681 aagaattacc ataccataat tttaaattag gaggttttgt attttgtact tatgttaaaa
     1741 atgaattatt atttaatgaa gtaaatataa gtaaagctaa tataagtaga ttaatatatc
     1801 tcgcaacata tctaaactat aatacaggag aagaaaattt attggttaga tattctcagt
     1861 ttaaagaaat gattcctata gatagaaaaa acatgagaga aatattaaat cttaaagata
     1921 gggcatttag aaatttttta gcagacgtaa aaaaatgtga attattatat gaatctaata
     1981 ataaatttta tataaatcca aagtatttta ctaaaggaat tcctaatttt gaaaataaag
     2041 aatatacaag aatatatata aatactagta gatttctttt tgaacattgc acaccaagac
     2101 aacataaaca attaagttat atatatcaac tcataccata tatacatttc gagagtaata
     2161 tcttatgttt aaaccctcat gaaacagata tttcaaaaat aaaaaaaatt agtttatata
     2221 aaatttgtca attattaaat attagtacaa taaaacaaga tatgaacaaa tttaaaaagg
     2281 aattacttag attttatata aaggtagaag atgataaata ttattttttt aaatatgtaa
     2341 tcgttgaagg gaataacaaa aatacagact atttcattat aaatccttgt gtaatatgga
     2401 agggaaataa tttaaattta gcaaagaaaa caattgattt atgctttttt aattgtattt
     2461 agaaagtctg tctaagaata agaggtaatg aactgtaaaa attaacaaaa atataggaaa
     2521 aacagttaaa aaattaaatt taggggtggc acagaaatgt gaccaaaagg caaaaaaacg
     2581 gcacagaaat gtgaccaaga aaattaattt aaaatcttat aatatagaaa aggagaagta
     2641 tgaaagagaa aaaaatagaa ttaatatatt tagaagattt agatgaatca gagaaaacag
     2701 gcgtacataa aaataggtat caaatattaa atgaggaata tgaacaaatg atagatacat
     2761 acacagagat gagtaaaagg tcttcatatg atattgtaaa tgaaaggcat aaggaggatt
     2821 ttaaggatga actatgggat tatgatgata cagattatga tgatggattg caaggaaagg
     2881 aggtataata ttgatatata ataataacaa tttttatgaa gaaatagatg gcaaatctgc
     2941 acaagattac ataaagaatt tagattatag catagacaat agcaaagata gaattaaata
     3001 catagaggaa cgattagggg taaaacacac gtggtttgaa gagtcaaata gaaattatat
     3061 agatacattt tataatactc ttacaaagag aaatgagaat attattacta gagtgaaata
     3121 tcaagaaaaa aaacaattca atgataaatt ttgggaaaat atatttgagc aaacatcaga
     3181 tagtccttta gataaagatg gtgtatatta tgttactatt gatggagaag aagtagttat
     3241 ggattataat agattcatta cttggtgtgg ctataatcaa attaatccaa tcgaatatat
     3301 aaatgttaaa aatcctttta gtaatagtaa tggtacatgg gagtacacac atcataatac
     3361 atcgaaaatt aagttgatat taaataaaga tgacagtata tattctactt ctaatatagc
     3421 aaaaacatta gaaataatgg ggagctatat actctcagta gatgataaac ctaaggaaac
     3481 taaaataaga atatacaatt caaaagagtt atttaataga gcatgtcagg aagaagcact
     3541 tctaaataaa atagcacctg caaatggtgg aaatataaat tcaaacactt ctaataaaga
     3601 tggattccta gaagacaatt cctttgcatt ttttcaatta cctaaaaact ataaaaaaat
     3661 aaaagatata aaagttaaac ctaaagatat taaaaaatat ccaattataa aaacatactc
     3721 agatacttat gaatggtata aagctaaata taaagaatta ggacataaac aattaagtaa
     3781 agaagagtta aaacttaaga gaatggttag gaaaaattta aatgcattaa aaaatgatat
     3841 gactgatgtt aaaaattcta tagagagacc aattatatgg aaagcaccat tagcagatgc
     3901 aggaagccca gaatgggact atttagatat gtttgataaa tcacatgtta aagagctttt
     3961 aaggattcaa aagggtaatg atttgcaaga tgatttaaca tgtatagtta tggatttaaa
     4021 taatattata tctaaaattg aatttaccac agtacaaaaa gatattttaa atttatatag
     4081 aaaagataaa tcattagagt ttatttcaaa tttaatgaat ataactcctc aagcaataaa
     4141 taatcaaata aataaaatag tgaacaaaat aatagatgca tatgagaaaa actatgaaga
     4201 aaattattat tatatatctg tttgtaaagg aaaatataaa aaatgtagca gatgtggaaa
     4261 aattaagttg ctatctaggt ttgataaaaa tggtaaaaag ggatatcgct caaattgtaa
     4321 aaactgtaat tagctggtgg ggaaaagtgg tttaaaaaag ggtcgcttaa aacatattac
     4381 ttatgtaagg ggttaataaa aataataaaa ttattcctta ctattatcaa atgaaaatta
     4441 tataagaaaa gggagattaa gaagatatga aaaaagtaga ttttttaaaa gaagtagcag
     4501 aagaattagg tgtatcacag ataagagtaa gagagatact tgatgtgata gaaaaaaaaa
     4561 gaaacgaagt actacaagat ggtgaagaat tgacgttgat gggagttaaa tacctaacta
     4621 aaatacaaaa aggtaaagaa ggagaaataa ctcttaaaga tggaactaaa aaaccttgga
     4681 aaactgagga aaaaagagtt ccaaaattaa aaatagctaa atctttaaaa gatgcaatta
     4741 tatagagagc gtatgggtta aaagcccttt catatggtga catatggtag caaacctaga
     4801 atatgctaaa aattaataca attagcagag atagctcatt ttgagaagct ctagtggctg
     4861 gtaataggta tcttatattg taagatgatg atgccactaa aaatattaaa ctacaacgtg
     4921 aaatttgtgg gataattact caccatttca aaactggaag aaagcacatg tcgtgagata
     4981 gcagtaagtc tttcttcctt ttaaataaat actaaaaggg agatttgata aaatgaatgg
     5041 tgaaaaactt atagaaaata aagaattaag agatagaaat atagatagag tagatgtatt
     5101 ggaacatgtg aaagagatat taactttagc aaatacagat tttagtacag tagaacttgt
     5161 ttctgagtat tatgaagtag atagagatac aataaaaaaa cttatacaga gaaatagaat
     5221 tgaattagaa ttagatggtc ttaagttata taaatacaat gatatagctg aagtacttga
     5281 aaatactaaa aagggactgg atgtcacttt taaaattcct ccaagaggtc taatattaat
     5341 acctaaaaga gctattctta gaataggcat gttacttaga gattcagaag ttgctaaaga
     5401 agttagaact agattactag atattgtaca tgatgcagaa gaacaaactg aaattgtaat
     5461 agaagaaatt agaaatgaac aaaatataaa agaagacatg cttgaagcga taatttctgg
     5521 tgaccataat aaattatctg tactacagac agaattaata gggttgaaga ataaaagaat
     5581 atctcattta gaagatgtta taactaattc agtcactata acagaaagta aagctattat
     5641 taataaatgt gttagaacaa tagcagttaa aaaatttaat tgtatgtttg gtaaagcatg
     5701 ggatgaattt tatagattta ttaactataa acttggcatt aatgttagaa atcgaaaagg
     5761 taaaggatta gctagatttt cagatgaaga aatcttaaag atggaaaaga tagctaaaag
     5821 ctggttagag gaaaatgata tagattttaa attggtaata taatttaaat ttgttttcaa
     5881 aagggatata gctcaggtgg ttagagcatt cggtttatac ccgaaaggtc ataggttcga
     5941 ctcctattat tcctagcaaa tatggagagg tactcaagcg gtaaagagga tagtttgcta
     6001 aactattagt tcaattaatt tgatacgagg gttcaaatcc cttcctctct tccaataaaa
     6061 agagtttgtt atttttaaat aatattttaa ttaaaaggga gatgttttta tggttaaata
     6121 taatgtacac aaggctttag cagaaaagaa gttattagaa gataaaatag atagatgcat
     6181 taataatttt aaaattgtag gaactaaaaa agggtctgat aaaaatgtat atgaaacaaa
     6241 aacaagtgta gaagatttta atgtagaagt ttcaagtaaa taccaacaaa tagaagattt
     6301 aatatataat tataacgcat tagataaagc aattaatatc tctaatgcta taacaaatgt
     6361 tcaaattagt aataaaaatt atacagtttt agaagcaata aaaagaaaaa atagtataga
     6421 attagataaa tccttgttga gacaaatggt tagtaactat gattgtatga tgtctgaagt
     6481 aaatcgtaga aatgaagaag tgcaaaaaaa tacagacaga atgtttgagg aaaaagaaaa
     6541 aagtaaagat ggagcagaac ttatttcatt ttataaaaaa caacaagaat ggtctttagt
     6601 agacccttta aaagtaagag agaaaataga aaaattaagg gatgaaatag aagaatttga
     6661 aaaagaagtt gattttgcat tgagtacatc aaatgcactt acaattatag atgtagattt
     6721 aagatagtcc ttaattggac tattttttaa tttgcaggtt aggcgaataa cataaattat
     6781 aagttcccat ttggttttgg gttaaaaatc aaactgctta tataaaagtg atgggtacat
     6841 acaaacgaaa cgattgtact gtataaaaga gaacaataga tataaagctt aaagttaaaa
     6901 attcaaatat aaaatctcaa ggataaaaga ttaaattatt aaaatagtaa aaataaaata
     6961 aaaaagttat agaaaatcct tgataaatgc taaatgcgtt aacttatatt agctattgtt
     7021 agctacaagg ctgcctaatt tgcaaataaa tatattccca gtagagttaa taaaattata
     7081 aaaaaggtga aagtgcatga aaaataataa aggaacagta ttagaaaaat gtgaaataat
     7141 aaaacaagaa aataatacat ataaaattat agaacatttg aaagatggag accatgaagt
     7201 attacttagt gatgttttgg ataagtatac tggtgataca gatttaaaca taacgataag
     7261 attaagtgaa acaatagtcc aataaatcaa tattggacta tttatttttt acttaataca
     7321 ttgataatga tatttatgtg ataaataggg agatattaaa tatggaagac atattgaaga
     7381 aaaaagaaga tgagtcacta gttgactata ggattagaat acgactagca aaggttaata
     7441 aagagataga tttggattgg ggagagatag ttgaactatt ggggttagaa tgttctccag
     7501 accattgtag gaaggtttcc tatgggttga aagaggcttt tgattatctt aattctaaga
     7561 tacagaataa ttctactcaa gaagagattg ataaaataaa tgagaaaata ttagaattaa
     7621 aaaagataaa agtacaacta tctgatgaaa ggtcattagt aaataagaaa ataagagaat
     7681 attcaagaat agataatata attgatttat ttaataataa aatagatgat atatcacttc
     7741 ataagccatt tctaagtgat tctagttaca aatgctatga atcttcaaat caagaggcta
     7801 ttatgctaat ttcagacata cattatggat tagagactat taatgcattt aatagatata
     7861 attcaaaaat attcaagata agaatacaat atttaaaaga taaaattatt gaatatagta
     7921 agttacataa agttaagaga ctgcatgtaa tgttacttgg agatttaatt tctggataca
     7981 tacataattc tataagatta gaaaatagag aaaatatagt tgaacaaatt atagaggtgt
     8041 ctgaaatact tagtgaattt atatatgagt tatctaaaga aattgataaa attattattt
     8101 attcagtagg tggaaaccat gatagagttc ttcctaaaaa agatgaaaat ttagataaag
     8161 ataattttac attattaata gatgaatata taaagctaag aattaaaaat ataaacaatg
     8221 ttatatttca agaaaatata tatgataatg acataatagt ggctaaaata tgtggaaata
     8281 cttgttttgg agtgcatgga gacaaagata aaatgtctac agcaattcct aaactgacct
     8341 ctcttataaa attaattcct gattatatct ttatggctca tctacacaat tgtaaggaag
     8401 ataattatgg agaaagtgaa atatgtgtaa atggaagttt ctccggaacg gattcttatg
     8461 ctaagaatct aagattaagt tcacacgcaa tgcaaaaatt aatgatattt aatgaaagtg
     8521 ggagaatatg tacttacaat ataaatttaa gtaagctata agttatttat taagagctga
     8581 gttaagttta tattgtaaat ttaattcagc tcttaattga gttgaatctc cctattaacc
     8641 actagagatt taattctcta gtggtctttt ttaatggtgg tgatgttatg aatggcaatt
     8701 agtaaaacaa agtgtttgaa ttgtggtgaa gaaaaatata cagatagata tttttggaaa
     8761 agtcatagtg aaatatttac tttaaataaa agacttccag tatgcaaaga atgttttaga
     8821 gctaggtttt tactattaaa tgggtgttac aatggagaac ttgtaaaggc actaaaacat
     8881 atatgtttca actttgatgt ttattttgat gaaaaattag ctaaagaact tgcagataaa
     8941 aaaaataaag atgaattaat agatgaatat atgaaaatta ttaatagaaa ttcaaaatat
     9001 aagggaaaga catctttaga taatttattg aaagaaatat atacaggaaa taataatgat
     9061 aaaaatatag tcataaatga tgaaattaaa ttaaaatggg gaagaggttt tgatgattat
     9121 gagtataaaa tcttagaaag aaaatataag gaatataaag aatattatga accagagtca
     9181 ttaactgaaa gaaaattgtt tgaagagatt tgtattattg aattagaaaa agataaatca
     9241 agagaaaagg gagatatgaa agcttttaat gatttatcta aattagtatc aagtaaaatg
     9301 caagatgctg aaataaagcc tagtcagaaa aagaaagctg gagattcgca agatgataca
     9361 tttggaatga aaatgatgat atatgaaaaa aatcaacctg taaaggatag gttaaaggaa
     9421 tatgaagatg tagatggatt tgaagcttat gtaaataaac atatgaaaaa accacttgct
     9481 gttgcattag gtttagctac aggtaagtat tcgataaatg atggcgataa agatattaaa
     9541 tttaaagatg atgtaagaga cattttagaa ggtaataaaa atgaggacta aatatgaaaa
     9601 aagagagctg tcatctaatg attcatcccg aaatctacta gagagtatag gagagtattg
     9661 gggtgctttt tatctcgcaa atccacatag attctgtatg gattactttg gatttaatct
     9721 acatttattt caacagatat taatttatat gatgatgaag tctgaccaat ttgtatttat
     9781 tgcttctcgt ggactgggaa aatcatggct tctaggagtg ttttgttgtg ttatagctgt
     9841 attaaaacca ggtacttgtg ttttaatagc agcaaaaaga aagaaacaag caaaattact
     9901 tattacaagt aaaattttag gtgatttata tttaaaatca gatactctga aaagagaaat
     9961 taaaagtttc caagtaaatg cacaagaagt atctatagat ttttggaatg gaagtagaat
    10021 agaagctgtt gtatctaatg atgatgcaag aggttacaga gctaatgttc ttattgttga
    10081 tgaatataga atggtggatg aaggaactgt gaatgatgtt ttagttccat ttttaacaaa
    10141 cccaagacaa ccagggtatt tacagaatcc taaatataga tatatgcaag aagagaataa
    10201 agaaatttat cttagttcag gctggtattc tcagcattgg agttataaaa agtttatgga
    10261 aacagtaaaa ggtatgctta gtggtgaaga tatgtttgca tgtagtattc cattcacttg
    10321 ctctttagaa catggactat taactaaaaa aagaatactt aaagaaatga aaaaagagag
    10381 tatgagtgat gcttctttca tgatggagta ttgtggagtg ttttataatg aatcagatga
    10441 tgctttcttc aagtcatctt gggtgaatcc atgtagggta ttagaaagta tgttttatcc
    10501 tcctagtgat atagagtatc ttgaaaataa gaaaaaaaga gataagaaat atcatcttaa
    10561 taagataaaa ggagaaatac ggattattgg tgcagatata gctttggcca gaggagttaa
    10621 gaatgataat tctatttata ctttgatgag gatgcttcca aatgaaggca catataaaag
    10681 atgtgttgtc catatagaag cttataatgg tatggaagct gaaaaacaag caataagatt
    10741 aaaacaatta ttttcagatt ttcaggcaga ttatatgata ttagatacac aaggtatagg
    10801 aacaactgta tggagctata tacagaaagc aaattatgat tcagatagag atgaatggta
    10861 tgatgcttac acatgtttta atgaagataa tactgttgac aagtcactag caaaaaaatc
    10921 acttcctgtt gtatattcca tgaaagctta cgctgatgaa aatcataaaa tggctatgtc
    10981 tttaagagat gtccttacaa atagaacctt agaacttcct ataagtgata ttgaagcaaa
    11041 agaaatgata ttagaaaaag aaatgataaa ggcagatgaa atagataaaa aggcagaatt
    11101 agaagcaaaa tatattgccc cttatcttca aacaacggct ttagtgaatg agttaataaa
    11161 tttagaatat agtgctgatg gaggaaaaat taaaataaag gaaaaaagtg gagcaagaaa
    11221 agatagatat tctagtttgg cttatacgaa tttcttagca gattatctgg aagaaaaaga
    11281 gaaaagaaaa aatagaaata atcaaaaaac tgttatgatt tattggtaag agatgaggtg
    11341 aataagtggg caaaaaaaat aataataaaa gcaagtctaa agacaacaat tctacacaaa
    11401 atcaaatcaa tatgctaaat gctcaaattg gaaaatatgc ctcagtaaaa gaatccacaa
    11461 cattagcaac agaaatgtat aagttaagaa gtataagcag agacaaatta agaaaagctc
    11521 tctccaatcc atatcaaatt tcaaatacag atatacttca ggatgcaagt atgatattaa
    11581 aagctacatc tggtacatat agaagagttt taaatttaat atctaatatg aatacttttg
    11641 accatatttt atacccaaaa gacataagta gattaaaaac aaaagaaaaa ataacaaaag
    11701 catatatgaa ttcggcgagt caattagaaa aatataatat aaaatttaca tcagcttgga
    11761 taaccgaaaa agtattggaa ttaggagagg tttatttata taagatagaa gataattcag
    11821 ggatggtttt acaacagata ccagcaaaat tttgtatgat aacaatgata gaaaataatg
    11881 taatgagata cgctataaat ataaaaaaac ttactgacaa aaacataata tcctttccag
    11941 aagaaattaa aaacatatat aaaaaatata aatctaattt acttacacga gaagaattaa
    12001 ttgataatac atattttcaa ttaagtgata aagcagtagc atttaattat gatttagatt
    12061 cagtgaaagg agttccattt ttttgcttta tatttgacga tttaatggag cttgaagata
    12121 tgaaagattt aaaaagcaca aatgcagtaa tagaaagtat taaattaata catcaaaaga
    12181 taccatttgg caaaaatgat gaaccactag tagaccttaa tttaattccc atataccaca
    12241 attcaacaaa agcaaattta ccaaaaggaa cagcaataac tacaaatcct ttagagttgg
    12301 aaacacatac tctttcagat ggaaaatcta aaataaatga ttatgtaaaa gaagcaaaag
    12361 agttcatatt tgataacgct ggaataaata cagccttact taactctgac aagataaata
    12421 atgaatctat cttaaatggt attattgcag atagcttaat tcctatgagg attcaacaaa
    12481 tgatagaaaa ttgggttaac tttgaactaa ataaagacaa gaaggcaaat ttatttaata
    12541 tgaagtttat tggaactact cactttaata aaatgaattt atccaaacag tatcgagaag
    12601 atatgggata cggagggagt aaaagcttgt ttattgcaag tactgggttt actccacttc
    12661 aagctataaa cactttacaa gcagaaaaat taatgggatt tgatgagttt ttaatacctc
    12721 aacaaacatc tcatactcaa agcagtggta gaccagataa atctgatata gggacagata
    12781 atggtaattc tactcaagca aaaggaaatg gggagaatga ttaattatga gtaagtttat
    12841 atatgctttt tctgaagatg ataagaaatt attaatggaa aaaggatatc gttttatatg
    12901 tgaaaacaag ttgaataata agactttata tgtttttgaa aataaatcaa aattaataaa
    12961 taatttcagt aatgaagaaa tgaaaagatt tatatttact agcaaaatac gtttttaaaa
    13021 ggaggtgaaa aaaataaaaa tattaaaatt accttgtaat ttaaaacatt attcagataa
    13081 aaaaaaattg gaagaactaa atagtaatct tttgccagtg tatatctatg ttatgcatga
    13141 aggtacaaat ccaaatggaa ctaaatttta tgaagaagct attgataaag ctgaaccaac
    13201 attaaaaaat gttccaattc taggatatgt aaaaataaat gaagatggaa aatatgactt
    13261 tgatggtcat aatgttttaa cacaagtagt tcaaacagat gaagggttta tattagaaga
    13321 atattatgaa gaacgaataa ttggcgttat accagaaaca aataaatatg aaaaagttga
    13381 aattgatggg cagaagtatg ttaaatgtaa aggatatata tataaatcat atagcaatca
    13441 tgcttacgat ataattatgg attctgatga aatagaaatt tcaatggaaa tagatataaa
    13501 tgattatcaa ttagatgatt cagatggatt ttataatata aaagactatg tatatcatgg
    13561 aattacatgt cttggttctg atgtaaaggg agctatgggt tcaaattgtt gtttaactaa
    13621 attttcaaga aaaattaatt ataaagaaga aatatcaaaa atatgctcag aaatttatgc
    13681 attagaacat ggagaggggg aaaagaattt gccaaataag aatcaaaaac ttaataaaaa
    13741 ttctgaagga tatgctttag cagtgagtaa tcttagtact gaaataagaa ataagttaaa
    13801 agaatacaaa gtcgaaactg agaattggta tggtgaaaaa gttgaagtac aagcttttta
    13861 ttataatgat ttaataccag aagagaatat tgctatagtt gaagatgaaa ttaactgggg
    13921 gtattattat ggtattccat atatagttaa tgaagatgct gtcattttgg attatgaaaa
    13981 taaaaaatca tatatacaaa cttggaggga aaaacaagaa ggagaaatag tacaggtttt
    14041 tagtagacaa gaaaaattaa aaaaagaaat aatagagaaa tttactgaaa agcaaaaaga
    14101 aatatctaat ttaaaagcca gtttagagcc attacaggca ttcaaagaag aaaaagaatt
    14161 tgagttattt aaatctaaag ttgatgatgt agctcaaaaa tttgaattag cagaagatga
    14221 aattaaagat ataaaaataa aagcttataa taaggaaatt acattagatg aatacaagaa
    14281 agaattgggt tacatatttg cattaaaaac tcttagcaat aaacaaagcg aaaaggaaaa
    14341 ttttagtata aatgatgata aaaataatac gataaaaata cctataagta ataataatga
    14401 tagttttagt gaaccagaag aaatatcttt tataaaaaaa tattcagata aagaataata
    14461 ggaggaattt acatgaagaa agataaagca ataatacaaa cagataaagt aaaaaatcct
    14521 gatgttttaa caggtaaata tgttgttcca tcagaggata ccaatccaat aaatttagaa
    14581 aatggtgctg taataaatat tggagaactt gaagacacgg agtatggtag ggatacccat
    14641 aaaatatata aagttacaaa tgacacatta gattggggta tagttgatga cccagcaact
    14701 atgtatgatg aaagattaga tgaaagagat tacgaagttt ctcctggtca gatatgtaga
    14761 tgtagacgac taaaaaaagg agatgaggta actatatctc tattacatat agcagataaa
    14821 agtattgcag taaaagacaa gttacaatta aaatctgatt cgtttcagtt tgaaaaattg
    14881 cctacagcag atgctaaaac tccagtagca gaagtattag aattatggaa ctatgaaggt
    14941 caagattcgg ttacaataaa agttttataa aatcaaataa aattaacaaa caggagataa
    15001 cagtctataa ctgttatttt tttatgcaaa aaaatagaaa aggaagtgtg taaattaatg
    15061 gcaacagcaa aaactttaaa gagaatagct catgagctat atacagatac atttagagaa
    15121 tatacagata gagatggaaa cacaataaca tatagagatg ctgaaagtgc aataaagaat
    15181 aaattaaaag aaatgatggg aggagaaaaa tataattatt ataaattcca agaaaataaa
    15241 tggggatttt ataatttaat atcagaatta atatctgatg atattaatag attaaatgag
    15301 gaagtattta gtccgttttg tgattttgag aactttgatt taggagataa aaaagaattt
    15361 actgttgaaa atactaattt atttaaagtt gcaaatatag cagatggtgt taatagtaca
    15421 agaagacaaa gattacttaa taaaaaagtt cctacaacag catttaaatt atctgttgct
    15481 atatatgaag aagcagagag atttataaca ggaagaataa attgggttgt atttgtgaat
    15541 agggtatcag attcatatca ttatgatatt gctagaagaa tagcgaagac atttgaaggt
    15601 gcatattcaa caataaatgc taagtttcaa gctacaggta attcagacaa agtattattg
    15661 gaattgatag ctaaggtaga aggggctact ggtaaaaagc ctattatata tggtactcca
    15721 cttgcattaa gtaatttaga aggtgttcaa actgatttag aaaaagaaga aaaaagaaaa
    15781 tatggtttta tacaaacatt tagaggtgga tataaagtac ttagtttacc aaatgcttat
    15841 gatgaaaatg cagcagaagg taaagagtgg gctttagata ataaggctat ctatgtaata
    15901 cctgatggag agaagataat caaacttggt actgaaggag atgtattggt catagaaaat
    15961 actgatgaaa aagaaagaga tgaccaacaa atagaatatt ttatggctca aaaaatacat
    16021 ttaggagttg taactgctgc taaatttgga gtttataaaa tacaataatt gaattagaag
    16081 gtggttatcc accttctttt ttttatggga gggagattat tatggcaaga gctaaaaaag
    16141 aaaatatttc taaaacagaa gtcaaagtta atactttaga aaaagatata aagtctactt
    16201 taaataaaaa agaagctaga ttaaatagat tagaactaca aagaaaacta aaacaaaaaa
    16261 aacaggaaat agatatagaa ataaaaaata tatccacatg ggatgttgat tatattgacc
    16321 caagagaaaa acaacagatt ttttctttat ctaaagtagg ttcaaaagat tcaactgaat
    16381 ttatagattt agatactctt tatagaatag taagaagaag tccaggtttt tttgaagaac
    16441 atagattaat aatttcggat gtagattctt tagatgttga atgtactcca atggacataa
    16501 tagattttct aggattgaat agtttatatg aatatataag aaatcctaat gaagactatt
    16561 tagaatattt cttatctgat aaagttgata taaattcttt tgaaaaaata ttaaataaaa
    16621 ataatgttga gttaacaaga agattagctg agagggcaat agatttacat aaaaagaaaa
    16681 agtttgattc tgggctaaag gctaaactat tagctaaaag aattggaatg gaagatttgt
    16741 acttgtttag ttaaaggtgg tggaatattt gggtacacca cttgtaaaaa tatataaaaa
    16801 gtttttggat ggtattaatg atgaagagat gttattacta tcaaatgaaa taatagaaaa
    16861 aatgatgtac agttatttag aagatgctat agttgatttt aatcaatgta aaaaagattt
    16921 gactataaaa tatgttgatg aaaaaggtgg agaaattatt cctgctgctc aattaagtta
    16981 tacatctaat tatagtaata aaaatgcaga gataactttg atgggaaaag atacaaaaga
    17041 agaatacgaa ttagataaag attatacaat aagctttgaa gatgaaaaat ttataataag
    17101 ttttgttgta gaaactacag aagagataat tttcaaatac aaatatttag gagaaatagt
    17161 atctgattta gatttagatg aaattaaaat attatcatat ggtatgcaaa tacattggtt
    17221 acaacctaag ataaatagag aagaaaattt aaaacagatg ctaactgatt ctgattataa
    17281 tgctaaatca ggtgctaata tgttggctaa acttcaagca agagaagagc aattgagaac
    17341 tagatttaat aaatatcaac aaagatacat gctaaaaaat tttgaaggat ggaactagca
    17401 tgagttactt aagtgataca aaaaaaagaa ttggacttgg ttgttcaact cctaaagaaa
    17461 aaagaatatt acaacttaga ttaagtttta agaaatatct aaaagaaaca ccaacctgta
    17521 ttgaagtacc aataactgat atagatgaaa tttgtataac agaagatact aaaagagcta
    17581 tagttgcaat taatgatata actaataatg ataaaagagc tttagatgaa aaaaatctat
    17641 tagttgaatc tgatttagat gtagatgtgg gttgttatct tttctatgat aattgttatt
    17701 ggttaactat atttaaagaa cataaagaaa tggatacata taaacatttc ataataaaaa
    17761 gatgcaatca attctttaat tataaatata aaggtcagat gtataaaatc cctatagctg
    17821 ttgaaaattt aaccttatat tctgatggta tggcagataa taaatatact tctatctcag
    17881 atacaaaaag acagctttat tttggaagta atcctgtcac taaaactata gatattgaca
    17941 ctagaataat gttaactggt aaaactgtat ttagagttac tagtattaat gactttgagt
    18001 ataatggcag agaaacaggg gcagatggtc ttattaaagc tatttgttta caagatgcat
    18061 tgatttcaaa agatgataca ataaataata ttgcttggaa tgatttatct gaaaatgaga
    18121 gtgtgattat tccttttagt aaaattatgg gtgatggatt tattaatcta ggcgaagaaa
    18181 atgaatacaa aatagaccat tctcaaggag ttgaatggct cttagacaag caatacggat
    18241 attgtaatat aattaatcaa gatgagaaaa aatgtgtcat acaggctaat acattggcta
    18301 aatattctgg ctttgaagcc ttgctattgg ctaaagataa agatacaaat aaaacaatag
    18361 atactaaaaa aataacacta agggggtaga tatatgggtt tatatggctt tccctaacaa
    18421 gatgataagt aatataggag caactctaat gtcaaatcaa gacttcgcta aatttatgat
    18481 ttataatgat gaatcccaaa aagatatttt atccatgcct gatataaaaa atcctgttaa
    18541 agaacttaga aacaaaaaag tatttctaaa tagaagagta gaaaaagtat taaaagaagc
    18601 agatatttca gtttttataa taatgtcaga atataggcca tgctttgaag gaagtaggac
    18661 aattaaaaaa acaaagatag aaattggtgt tgtttgtcat gatgaatgcc aaagtacagc
    18721 aaatggctta agagatgttg cattagtatg ttgtatagtt gatattgtaa ctcaaaatga
    18781 ggaaatagct ggtataggta agattaagtt agaaaatgta tatcaaatgt ataatttaaa
    18841 tactgattat aacggatttg ttataactgt atcagctgaa agttttggtg atatgtaatg
    18901 ttggaaaatt attatatcac aggattacca attaaactag gtgaaacatt aggaactgta
    18961 tatcaaccta ctattgaaga acttattaaa tttgatatgg ctaatttaga gatagttaat
    19021 ccatttttag tacttgaaaa gagctattct cagctatgta acgagaaaat atttgaattg
    19081 aaatgtaaat atgatgctat acctatcttg gattcttctg aagaaattga gttacttagt
    19141 gataagataa aaaaatcttt atctatatta tataaaactg atattaaaaa tattgagtat
    19201 atgaataata ttaaaattgg tattttaatt aaatttgatg ataaaaagaa aaatgctttt
    19261 ataagtagag aggattttgg actggtatca gatttgattt tagagatgtt ttgtattgat
    19321 aaaaagaatt tatttaaaga tgatgaagat aagtggattg aaaatactgg ctctgaaaga
    19381 gaaaaacagt tgattgcaca ctttaaagag aaggaaagaa agaaaagaga aaaagaagca
    19441 tatcacttat gtgattatat aaatgtagtt caaaacatag atggatatgt accaatagat
    19501 gttattctaa aaatgactta ttggcaatta attaatgctt ataagactaa gatacaattt
    19561 aaaaattatg atgaaagttt aggctttgca atgtctttta aatatcaagt tgaggcagac
    19621 aagattaaac attggtctaa agaaataaaa atacaaacta gcactgttaa ataacagtgt
    19681 tattttttat gcaaaaaatg agaggatgat gaaataatga gatttgcgat aaaagatgca
    19741 agtaatataa tagtaaaaaa taaaacaact ggagaaccac ttttttatac agcagattta
    19801 aatgctttta atttcaaatt agattcagaa agcgtgtacg caaaagctaa aggtgcaaac
    19861 acaatagcat ttgatggagc tataacagca agtttagcac ttgaacaaga ggtaatacaa
    19921 atgccacaac tagcaatgct attagcttct gatatggttg aagaatcggc taaggttggt
    19981 aaaagaaaat tactaacttc tgatggtact aagaaagtta ctttagaaaa tgtaaaacca
    20041 gttactaata gtatatctgc atatagtgtt gaaaaagatg gtatatcttt agttaagaaa
    20101 ttacagttta cttcaactgt aacaggctct aacacagaaa taacaatatc tacagctgat
    20161 tttaatgcag gagataaagt agcagtgttt tacttagagg aattaccaaa ggcaaaagtt
    20221 ataaaaataa aagaagagtc tactgctcca aactatgtaa tagaagcaga tgttatggct
    20281 aaaactgctg atggtgaata tatggtctta tatatgacta taccaaatgc aaaggcacaa
    20341 agaagtatag aattaaatct tactgctgag aatccatctg gatttaatat gacactagat
    20401 gttttgccag atgaaaataa agaatatgta gtattcgctt ttgttggaga tgaagatgtt
    20461 agccctgtta gaatggcttc tatgttaggt gctgaattaa tagacgaaaa ggatgttaaa
    20521 tctaagaaat agtgaatttc cctactcaaa agagtagggg ttattttttt acgtttaaat
    20581 ctggttttaa tcggatttga acttaaaaaa atgaaagaag gtgataaagt ggtattttta
    20641 gatgataagt ttttatttga taatatttca agtgaatcca tgaacattaa attagttact
    20701 ttaggtgatg atgaaatact aaatgaatat ggattgccct acgaagaagc gattaaatct
    20761 gatagtactt ttaataaaaa tccttgttac tcagaggatg aaactactat tgaacctata
    20821 acactacaat tctgtttgtg tagcgaacat ggggaggctt acgaatggga tgactatacg
    20881 ctagaaaaca tatgtaattg gttttggcag agagaattta aaccttttat cagttatgac
    20941 aatatagaag aaatatacta tctcaaagcg agaaaaataa taaaaagata tacaaaagat
    21001 aaaaaaggcg tattagaaat agagtttcaa ccatatacaa attatgctta tagaagcttt
    21061 caaaaagtta taactgtcaa ggatacaagg gaaattaaac taaataatgt gtctaatgta
    21121 gatgaagaat atgctccagt aattgatatt gaatgtctaa aagaaggaga tataactatt
    21181 agaaattcta ctatcagcga taatgaagaa gacaatttag ttataagtgg attagaatta
    21241 aatgaaaaaa taacaataga taatttatac tatactgttt taaatagttc tggtgagaat
    21301 aggtttaata ttgttaatag aaagtggatt aggttaagaa gaggtgtaaa tatattgaag
    21361 tttactggaa actgtaaagt ttctattaag tgtaaatatc caataataaa ataagggaga
    21421 gatagatatg aataaaatac aagtagataa attaatacaa gatgaagtta gggctataat
    21481 accaattata gatgagaatg gtaaagagga atatatagaa gttagaaatc ctgataagga
    21541 gactaaagaa gagatattaa ataaaatatg ggttggtatg gagaatcctg atttagcatt
    21601 gtctcaagaa gatattctta aaatgttgat tgataaattg actaatatag agttaaatat
    21661 tgaaatagaa gatttagtag atggcaatat atctagtgaa ttagaaaatg tgatgtatta
    21721 cataggtcaa atagaaaatg aattaacggc atctttatta atgaatactg aaattaagct
    21781 aggtcagatg aagaatgaga tattacagga cagagtttta aaagagactg aagaaattga
    21841 aaaaatgaat aatattaaag aaaaggtagt gaattagatg gtatttaaat cattagatga
    21901 attggttgct tatacaaaaa ataagattgc tgcatctatg cctgaagttg gaaaagaaat
    21961 gaaagagata gtaaaagaag aagtaaacaa acaagtttat tctgattacg ctccatcact
    22021 ttatgataga acttatgaat tacttaatgc aatagatgtg tctgaaataa catcagattc
    22081 tgtagctaca gaaattaaag atagtgggag ctggtttgat atatatagca aagcacatgc
    22141 ttttccaatg gagcgatttg aaaatggagg tgtgtgggga catggaagta ctaaatataa
    22201 tcctgtattt agacctagaa caaatataat ggaggaaagt aattctaagg cacaagagag
    22261 agtaccaaag tgttttaagt ctaaaatgaa tgaattaggc gtgccagtaa aatagacaat
    22321 atcgagtgtc tattaagtta tagagttttc taagggttgt atgttatcca ctagtagatg
    22381 tatatcataa gtggaaaatt aaaaagcggt gatgctctaa catatagtga gtaatttagc
    22441 ctagataggt agtttagttg atttgcttag ataagtggtt tagaattgtt tctcaatgtt
    22501 ctaatacatt gattgatgca aaaatatata caagtatatt gtgaaatata cttgtatata
    22561 acatattgat ataaagagag aggtatagtt gtttcaatta acatttaaat ttgaatatat
    22621 cgaggaagta ttagaagtta caagagaatt agattatcct aatatacatt ttagggaatc
    22681 ttgtggtaag ataatagaaa agtaagaatt aaaaaaagat agttttacaa ataataaagt
    22741 caatattcca aaacatttaa tatataatta taatgatgat atttggactg gagatataat
    22801 aaaaatggct aatcaggcaa agaaaaatga ttatagcttc tttatgttca atgatataat
    22861 ttattttgta gatgatgaat gtataagtta taagataggt tttagagatt aaattgtaaa
    22921 atttataaga cgtttattat tattaaaata ttatagataa ggcgagtggc catagtacac
    22981 ctttaaggtg tggagataga ataaaccttc tattatctta taataataga aggtttattc
    23041 tattgtaata taaatatcaa gatgtattaa taaatattaa agataatcaa tttagattgt
    23101 tttttaaatt gaatcattta tgttgtagtt atttcaatga tgacatgtgt atgacaaata
    23161 taagacactt ggaaatattg aaagacatat atatctttat tgcttagaat cgaatatagg
    23221 aggttgattt ttatggttga aagtattgga atttcaacaa gtgttcctga gaggaacatt
    23281 ttgtttggtg tttagccacc ataagagctt attttgatgt ttgagtggtc actactttga
    23341 gtgtgatagg attgaagagc tactacttga gggtagatga tttactggaa tggtttatca
    23401 tgtgaattaa attctgtagc ttctaactag ccttaagttt aagggttacc tatttataaa
    23461 aaatgataga taatgagatt taatctgtag tcaaagatgt agtagaaaaa ataggatata
    23521 aagatgtatc tgacaccttg aaaaacatat tgatgaaaaa gttaaaaata ttaaggcagc
    23581 ttatatagct gtcttttttg tatagaataa aaaagtttat tataacagat agttattatt
    23641 ttattagtgt gttaccaaga ataaattcct tttatctaac atttattaca aataatatat
    23701 atttttatct aatgaatgat tttatatttt agtgtaaatt ggtatat
//
LOCUS       DAMJVK010000060        23712 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.061, whole genome shotgun sequence.
ACCESSION   DAMJVK010000060 DAMJVK010000000
VERSION     DAMJVK010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23712
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.061"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(536..1408)
                     /locus_tag="PC414_002419"
     CDS             complement(536..1408)
                     /locus_tag="PC414_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2464807.1"
                     /translation="MEETNEMKDSKHLISNIQSQYTRLSKGQKLIAQYILNNYDKVAF
                     MTACKLGETVGVSESTVVRFANALGYSGYPKLQAALQELIKNKLTTVQRVEMAHDYSD
                     DFAILNKVLKSDIDNIRSTLEEIDERAFKEASNKLLRARKIYILGMRSSFVVAQYLGF
                     YLDIILDNVHIIRMDMGDAFEQIVRINEEDVIVAISFPRYSKKSYQIVNYAKEKGAHV
                     ISLTDSLFAPVASLADNTLLVKSNMASFVDSLVPALSISNALAISVGMKEKEDIKQHF
                     DDLEQIWKRYSVYE"
     gene            complement(1802..2380)
                     /locus_tag="PC414_002420"
     CDS             complement(1802..2380)
                     /locus_tag="PC414_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDF2464808.1"
                     /translation="MAYAKYLTKITDINKFYLENIINEGDIVIDATMGNGYDTRYLAE
                     KVGEKGFVYAFDIQEEAIKSTRKKLEKEGYTDRVKLIFDGHEKMNQYIKEEVSCVLFN
                     LGYLPRAKHSIITKPDTTLKAIKLSLEMLKENGVISIAIYTGHDGGQEEKDCIYNLVN
                     NLNQDEFNVLESKFLNQVNNPPQLILIEKKKA"
     gene            complement(2438..3154)
                     /locus_tag="PC414_002421"
     CDS             complement(2438..3154)
                     /locus_tag="PC414_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDF2464809.1"
                     /translation="MRINKYIASCGIASRRKAEEIILGGRIKVNGSVVKELSFNIDEE
                     KDIVEFDNKKVKPSENYIYIVLNKPEGYITTVKDQFNRPSVIDILKDVRERVYPIGRL
                     DYETSGLLILTNDGDLTYKLTHPKHEIDKMYVASVKGIISDDEIRQFETGLKIEDYTT
                     APAKIRVTKENKEKNYSVCEITIHEGRNRQVRKMCKAINHPVLNLRRISVGKIVLKDT
                     KVGEYRYLTEDEIKYLKSIK"
     gene            3345..4430
                     /locus_tag="PC414_002422"
     CDS             3345..4430
                     /locus_tag="PC414_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDF2464810.1"
                     /translation="MIIISKENIKYYLIVVFIGILFFKFINTPSDFISSIEGFLKFFS
                     PFLLAILLALLLNPLVMLFEIKFKAHRLLAIFLSYIFIGFILAFAIRLLIPSIANTLN
                     RLINEMPMYTDYIDSFIEKNMSNIYFLKTLIPHIQHSLDNLLKEASNFVGKIPKNFLI
                     YTLSISSMLFNMTMGFILSIYIIYDKEKIALGFKRFLYSSTARNKADNIIEFFRTTHD
                     IFYDYLLGRILDSLIIGIIAFLGFQFVIRIENALFLASIIFLGNIIPYFGPFIGAIPP
                     IAMTILYSPQKTIWVIAFLFILQQLDGNFIEPKVMGNQVGIGAIWVISAILVGQSLFG
                     FIGVFLSVPIAAVVKTYVDKYIDNRLQ"
     gene            complement(4585..5244)
                     /locus_tag="PC414_002423"
     CDS             complement(4585..5244)
                     /locus_tag="PC414_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDF2464811.1"
                     /translation="MRGRVILLISILSIFLVGCSFNKKDEINLVEQGKTYLEKHNYKK
                     AMESLSSALEEDSTNENARAMYMQAMRMSNMTEFEELKNYEGAIKELKLIEKIKNGSS
                     VIKEEATKKKEELTKLEKEQNEAEEQRKKKAKDTAGEDRYRVESDARKSSYSGSSKNN
                     KSSSKNNDKKSDSNKSDNNNSSSQNQEKPNNSNPTPAPTPQDTTGGSDSGGNSQNSNN
                     Q"
     gene            complement(5461..6753)
                     /gene="lysA"
                     /locus_tag="PC414_002424"
     CDS             complement(5461..6753)
                     /gene="lysA"
                     /locus_tag="PC414_002424"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HDF2464812.1"
                     /translation="MKLHGTMTVNNDELYIGGVSCLELSKKYQTPLYVFDEALVRQNC
                     REYIEYFKAREGKNKVAYAGKAFLPLYMCNLINEEQMCLDVVSGGELYTAYKSNFPME
                     KVFFHGNNKTKEEINMGLDLGVGKFVVDNFYELDLLEALCEDKGKTQEIYFRITPGIE
                     AHTHDYIKTGQIDSKFGFALVNGDLFRAIEKLSEYKNIKLVGLHAHIGSQIFDVEPYI
                     DTVDIMMNLVKEIKEKFNIQLTEIDLGGGVGVYYSEGDKPKTIKEFCETIITKAETSC
                     KELGIEVPTLVIEPGRSIVANAGSTLYTVGSIKDIKDVRVYVSVDGGMTDNVRPSLYQ
                     ASYECSIVNKINHEAMNHVTIAGKCCESGDILIGDINIMDIKSGDILITTSTGAYGYS
                     MSSNYNKIPRAAVVSVFDGNDKIICKRQSYEDLLALEI"
     gene            complement(6771..7964)
                     /locus_tag="PC414_002425"
     CDS             complement(6771..7964)
                     /locus_tag="PC414_002425"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HDF2464813.1"
                     /translation="MSIIVQKYGGSSVADTEKIKSIAENIIERRKENPQMVIVVSAMG
                     KSTDEYITLAKELSNEPSKRELDALMSTGEMISASLLSIALNALGCKAISYNAYQLNI
                     KTSGLHGKSQIDDINVNRIKNSLDEGNVVIVTGFQGINEDGDVTTLGRGGSDTSAVAL
                     AVKLNGKCEIYTDVDGIYFTDPRKYSKASKLDEIEYEEMLELASLGAQVMHSRSIELA
                     QKYGVEIYVGRTCGTEKGTYIRGGKDMKLEDKVITGLATSDDDSSITIKDFKAENISS
                     LFEDIATIGISVDMISQTAPILDKISVSFTVPKEELGECKKIVSKYTDEEHLVIDNNI
                     TKFSLVGLGMKNTSGVAAKVFKIFNENGIMIKLITTSEIRITCAINSDDKQVAIEKIA
                     EVFNI"
     gene            complement(8325..8942)
                     /locus_tag="PC414_002426"
     CDS             complement(8325..8942)
                     /locus_tag="PC414_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464814.1"
                     /translation="MTNMEKEILLLISRVRCVTESQFRKIYGDQKRYNKKNFKKTLRK
                     MCSEYTLKKYPCDVDYYNYKDLSYVYYLNGSKLFKGDDLIKALVGSELVVRINTAGCD
                     VKRFYRNVSVDDVKYDLFIEYTDQFGNIKQVLVDVDLNNEIDISKYENLSKKIDKSTI
                     PFFKVPNVLVVTPDNLSELNDLERYDNEYSINFIDLGLNKLFNCI"
     gene            complement(9112..10812)
                     /locus_tag="PC414_002427"
     CDS             complement(9112..10812)
                     /locus_tag="PC414_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464815.1"
                     /translation="MKRYLKTYGVALIIVIIYAFIKLPVLRLDFTSLFSVGIIFFVVA
                     GILDMMLDRNERASKMAKYNFSIAIALLVYIVVVPFITSTPVLHAKSYRELLGKVTES
                     KFTDDISPVSVNDIRLVDEDMAMKLGDKKLGEVPAIGSVSKLGKFHIQNVDGELYWVA
                     PLVHRDIIKWITSLSGTSGYVKVSASNPQDVQLVQEIDGKPIKIVYQPEAYLHQDLQR
                     HLYIHGIVNVGMTDFTLEINDEGRPYWVVSLYEHKIGYGGANATGIATVDAETGKINV
                     YDVKNTPKWVDRIQPQSFVTDQIKDWGVYVNGFLNSVISEKGVLVPTEGTSLVYGNDN
                     RSYWYTGITSSGGDESTIGFMLVDSRTKEARLYKQPGATETAAMKSAEGNVQEKNYEA
                     TFPVMYNILGQPTYVSSLKDKAGLVKRVAFVSVEDYNVLGIGEDKNEALRNYKDALES
                     KGNDLKLDNDLKDEVLEGTVTRISPDVRGGNTNYYVTLDSNKDIIFRATSKVSSELPL
                     TQVGDKVKISYSKEESGVIEMSEFDNLNIANFEDKKAKEKTGTDENTDSKDSENQGNV
                     "
     gene            complement(11209..11772)
                     /locus_tag="PC414_002428"
     CDS             complement(11209..11772)
                     /locus_tag="PC414_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464816.1"
                     /translation="MNKVYIVTTYTGTILSYLIRNISKKLYTHVSISLNENLKPMYSF
                     GRLNPRNPFIGGFVEENINQGLYAIRKNTVCRVYSLEVDNLQYENLYKNIKLISDYRE
                     DYYYDTMALIYLLMNIQRQSEYKYVCSNFVADMLEKSEIDILNKQAFEVTPNDFYNLS
                     GLTLEYEGLLSEYNSRQHSYVSKIANI"
     gene            complement(11931..12644)
                     /locus_tag="PC414_002429"
     CDS             complement(11931..12644)
                     /locus_tag="PC414_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2140 domain-containing protein"
                     /protein_id="HDF2464817.1"
                     /translation="MGKVAKILLSLIVIVLVLAGIVVYILTPKERYDVSSQNNKIIDE
                     EYLSKGSYINSMEVVKNPLRMVGKISVSEEEFKNLIYTLMQKHGINELENNFVEMKNG
                     KIKVAGPYKVFGLVNSQYELELRPTLKDGNLVVSLENVKIGKFKLSDKMLEKILSNYN
                     KKVPFEVNGNKITLEKSYLYPITLKNISIKKGNVDLDMEVEIDLKSQINGALGYLKDS
                     GKVQDILNHLINLKKTSVG"
     gene            12936..14600
                     /locus_tag="PC414_002430"
     CDS             12936..14600
                     /locus_tag="PC414_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HDF2464818.1"
                     /translation="MSNETNSSNFIKNIIINDLETGKHDSIITRFPPEPNGYLHIGHA
                     KSICLNFGLAKEFNGKANLRFDDTNPLKEDVEYVESIKEDVKWLGFDWNELNFASNYF
                     DEMYKRALILIKKGKAYVCDLTQEEMREYRGTLTEPGKESPHRNRTIEENLDLFERMK
                     NGEFKDGEKTLRAKIDMSSPNINLRDPIIYRISHSTHHNTGDKWCIYPMYAFAHPIED
                     AIEGITHSICTLEFEDQRPLYDWFVKECEMENIPRQIEFARLNINNTVMSKRKLKQLV
                     DEGIVDGWDDPRVPTISGIRRKGYTAEALRNFCSEIGVSKVNSTVDSQMLDYFLRENL
                     QPKAPLTMGVLRPLKLIITNYPEDKIEMLEIENNAKDESQGKRLVPFSRELYIEQDDF
                     MEEPVKKYFRFFPGNEVRLKGAYFVKCTDVIKDENGNVVEIHGTYDPETKSGSGFTGR
                     KVKSTIHWVDAKSAIPCEFRLFEPLILDDIPENEGKHFLKQINPNSLEILQGFVEPTQ
                     IKDAKPFDKFQFVRNGFFSIDNKYTTDEKFVFNRIVPLKSSFKPGK"
     gene            complement(14707..15606)
                     /locus_tag="PC414_002431"
     CDS             complement(14707..15606)
                     /locus_tag="PC414_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HDF2464819.1"
                     /translation="MKKVKLIYNPNSGEKKILSNLDTIIELYQEKNYLLIPYRLTIKE
                     PVKNAFKEVDSSYEHILIAGGDGTVDLVVNAMKELDIKIPIGILPTGTANDFSNALQI
                     SFDIKEAINEIINSKPKKIDIGKVNNKYFINVASAGMFTDVSQRINTDLKNSFGRISY
                     YIKGIEEALYMRKFKIKVSSEEMYYDGDMYLMLIFNGKTAGNINLAYKAEIDDGYLDV
                     IIFKGMPIPKSIPVLISVLRGDYLDQYNGNEILYFKTKKVHIECEDSLITDIDGEKGP
                     DFPLNIECIKDGIEVMGICSEKS"
     gene            complement(15633..16271)
                     /locus_tag="PC414_002432"
     CDS             complement(15633..16271)
                     /locus_tag="PC414_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003356606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HDF2464820.1"
                     /translation="MDQNLNWLNEKRIEKTIKSLENNNMKGYLVSNKEELLKKIEEIV
                     KAGSLVGCGGSMTLFETGVIDFIREKYNFLDRYAKDIKPEEMKEIYRKSFSADAYFTS
                     TNAITEDGELYNLDGNGNRVAAMIYGPDKVIVIAGVNKIVKDVDEAIKRTKTIAAPAN
                     CKRLSTNTGCKTTGICIDCSSPGRICCSYTLIKRQLTPERIHVIFLNEDFGY"
     gene            complement(16608..17606)
                     /locus_tag="PC414_002433"
     CDS             complement(16608..17606)
                     /locus_tag="PC414_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4003 domain-containing protein"
                     /protein_id="HDF2464821.1"
                     /translation="MEQSFINGKVNLLIENYKALNEVKGSWQMGLIQHSCALAFTLKN
                     KRISPRLVEERIELIKKNTGLFSNFRGYNMFYMATLLSFESNPESSFKMILDIYKELK
                     SEKFWGDTYLPLTASIVYENREKMDYLTCISKMKIIYDYMRKKHPFLTSSDDYCNIAL
                     IAIHSKNLDEDLEYIEKCYEFLNENGFYKGNDLQALSQILLFSDDRTMLKCKKTIELK
                     KAFKENDCKMNYYGYPIIGAISLLDYREDEIIEEIKNVSNKLKEEKGFGNWSLGKSNR
                     VMISSAIVASIYADLIRNENNIGSITNNIFLNIVVAIQIACVMAATSAAIASSSSN"
     gene            complement(17704..18279)
                     /locus_tag="PC414_002434"
     CDS             complement(17704..18279)
                     /locus_tag="PC414_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464822.1"
                     /translation="MKLKEALITCVVCSVCLTPLLVNGETKIDADHCKESIVNIDTIV
                     KSGKSFNSKYNQGMEDYKNSDEYKLKVQKEKLQNQLGIKIKKLVPITFEVSYYTSLNQ
                     ENGYGAITATGDDLEDGFVANNHLSFGTKIIVDGKLKTVMDRGSSKYFSNSNAIDVFV
                     PKWSNESESEYYDRVNNMGRHYKKGYIIVEE"
     gene            18595..18858
                     /locus_tag="PC414_002435"
     CDS             18595..18858
                     /locus_tag="PC414_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464823.1"
                     /translation="MIKNTKQITVEVNEILLQEMSKITNDENNILTDLINESIVNYIL
                     ENKKHNIENKDLEEVIKFYKKENTEKPKSAARAIAEAKTHTLY"
     gene            complement(19295..19672)
                     /locus_tag="PC414_002436"
     CDS             complement(19295..19672)
                     /locus_tag="PC414_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2464824.1"
                     /translation="MTITKIPPAELKVMKFIWSSDFTVASKDVIEAMEKLYNWKQTTT
                     LTLLSRLVNKQFLSARKIDRHTHYTVLIGEEEYLHFETKTFLDNMHGSSIESFMKSLF
                     KKGVNEEELNSVEKWVKDIKKDE"
     gene            20013..21125
                     /locus_tag="PC414_002437"
     CDS             20013..21125
                     /locus_tag="PC414_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF2464825.1"
                     /translation="MNAFIRKRNKNYVVYLEFEDEKTGKRKQKNMGTFDKKREASKRL
                     NEVKESLYNDGFLVPSEITVSEFLLDFLNKYSENISLATYNNYVSICKNYINPSIGKY
                     KIQDLHPVHIQNYIDKLSYKLNPQSIKIHINILKLAIKRAYRLKLIKENVMDSIEAPR
                     YKKFKNEIYDREQMIKLLDLAKNTEMELPINLAIGLGLRISEVLGLTWDNIDFEENTI
                     TVNKITSRINGSVILKEPKTESSVRKISSPKELMSLLKEYKIKQNKNLLKSSIRNNNN
                     LLFFNKKCEPIAEDVMSKKFKRFLEKNELPHIRFHDLRHSHVTLLINSKVPIKVISER
                     VGHSNISTTLSVYSHVLKEMDKEASDKISESLFNAN"
     gene            complement(21333..21809)
                     /locus_tag="PC414_002438"
     CDS             complement(21333..21809)
                     /locus_tag="PC414_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464826.1"
                     /translation="MVKKRLVAVVISLAMISVGNFSYAHSGGHNSSGGHHNKNKSELT
                     RYYCGGHSAHQHNKGFCPYASTKENSGVTISSVHNEKNHKNYFAEKGYNKGYEDGCKG
                     LYNLSVTEAKSDNLYEKEILKSSYEKGYLIGYNEYKAKKVDDVMKKMLDIPMLASI"
     gene            complement(22008..23633)
                     /locus_tag="PC414_002439"
     CDS             complement(22008..23633)
                     /locus_tag="PC414_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2464827.1"
                     /translation="MLQVTGVGLRFGDKELFKDVNLKFTKGNCYGIIGANGAGKSTFL
                     KILSGEIEPNTGSISITDKERMSVLKQDHFEYEEETVLNVVIRGHERLWNIMKEKDAL
                     YMKEDFNEEDGIKAAELEGEFAELDGWDAETNAEKILMGLGITKDMHYKQMKELVGGE
                     KVKVLLAQSLFGKPEILLMDEPTNHLDFKSINWLNNFIMDLEESIVIIVSHDRHFLNQ
                     ICTNIVDVDFGKIQMYVGNYDFWYESSQLALQLAKDQNKKTEEKIAQLKEFIARFSSN
                     ASKAKQATSRKKQLEKLEVEDIQPSRRRYPYVGFTPAREIGNEVLEVHNLTKTIDGVK
                     VLDNVSFRLDRDDKVVFMGDEIATTALLNIVMGELEPDSGEYKWGVTTSQDYLPKNHN
                     KFFDGVEYSLVDWLRQFSEEKSESFIRGFLGRMLFSGEEALKEAQVISGGEKVRCMLS
                     KLMLSNANVLVLDDPTNHLDLESITSVNKGLEKFPGVLLFTSHDHEFISTIANRIIEI
                     TPNGIMDRKMDFDEYLESKEIQDQLAKMYGKDK"
BASE COUNT         7900 a         3857 c         2487 g         9468 t
ORIGIN      
        1 tacaaaagct ttatattcta tgtcttaata tttataaaaa ctttatttta tttttataaa
       61 gtattatatt tctaaaaaag tttccctttc cccaaaaatg gctacttaaa aaatcaagat
      121 ttcataccta actactaatt ctatgatttt taaatttttc aacttctttg tcaaagttaa
      181 aataaaatac taaaagacac atattatagt gaacaaaaaa gatagaaata catattttaa
      241 tataatttta ataagatatt atatacttaa atacaaataa aattaatttt ataattgtta
      301 attaaggaaa tgatattgtt tagagaaata aaaaaagcac tctccatgaa tggaaatgct
      361 ttctttaata aattagttga cgtagtaaag atgtattcag aatacatctt tatgttttga
      421 gtctcgaaat atttatttgt attatttaaa cgaaaattta ttttttaagt gataatcgag
      481 tatttactaa taaggctatg attctcaatt tcatagcctt attaatatac aaactttact
      541 catacacaga atatcttttc caaatttgct ctaaatcatc aaaatgctgt ttaatatctt
      601 ctttttcctt catcccaaca gatatagcaa gggcatttga aatactaaga gctggaacta
      661 gagaatctac aaatgaagcc atattactct taactagaag agtgttatct gcaagtgatg
      721 ctactggcgc aaataagcta tctgttaatg atattacatg ggctcctttt tctttagcat
      781 aattgacaat ttgataagat ttcttagaat aacgagggaa actaatagca actattacat
      841 cttcttcatt tattcttaca atctgctcaa aagcatctcc catatccatt ctaattatat
      901 gaacattgtc aagtattata tctaagtaaa aacctaaata ttgagctact acaaaagaac
      961 tcctcattcc aagtatatat atttttctcg ctcttaaaag tttgttagaa gcttctttaa
     1021 acgctctctc gtcaatttct tctagtgtag accttatatt atctatatca ctttttaaaa
     1081 ctttgtttag tattgcaaaa tcatcggaat aatcatgtgc catttcaact ctttgtactg
     1141 tagttagttt attttttatg agttcttgaa gtgcagcttg taatttagga tatccactat
     1201 aacctaatgc atttgcaaat cttacaactg tagactcact tactcctacg gtttcaccca
     1261 atttacatgc agtcataaat gcaactttat cataattatt taaaatgtat tgtgctataa
     1321 gtttttgacc cttactcaat ctcgtatatt gagattgtat atttgatatc aaatgtttac
     1381 tgtccttcat ttcatttgtt tcttccattt ttttctcctt ttagttatat aaactatttt
     1441 gttttattaa cactgtcttt ttctctaaaa attttatttt ttatctcaat ttcatcttag
     1501 tttttatcat agcttcttaa actcataaaa ttctaacttt tctgtacctt atacttcaca
     1561 tagtattttc gattaaattt attataccat ctttccttgc attttttaat atttatccaa
     1621 tatttctttt aaatttttca atttttactc atttaaaagt tatctatctt attttgcata
     1681 tttagatgta tattttgttt tgagaataaa ctgttgtata cttattattt aaagtatttt
     1741 taataggtca cactatatat attaactctt aatacaatct tgtcaagttt tttagtattt
     1801 attatgcttt tttcttttca attaaaatca attgtggtgg gttattgact tgatttaaaa
     1861 acttagattc taaaacatta aattcatctt gatttaaatt attgacaagg ttatatatgc
     1921 aatctttttc ttcttgacct ccatcatgcc ctgtatatat ggcaatgctt attactccat
     1981 tttcttttaa catttctaaa cttaacttta tagcctttaa agttgtatct ggtttggtta
     2041 taattgaatg tttcgctctt ggaagataac ccaaattaaa taaaacacat gaaacttctt
     2101 cttttatata ttgattcatt ttttcatgcc catcaaatat caattttact ctatctgtat
     2161 atccttcttt ctctagtttc tttcttgtag actttattgc ttcttcttgt atatcaaaag
     2221 catatacaaa gcccttttct ccaaccttct cagctagata ccttgtatca tacccatttc
     2281 ccatagtagc atcaattact atgtcacctt catttataat attttctaaa taaaatttat
     2341 ttatatctgt aattttagtt aaatactttg catatgccat aatactattt ctccctacta
     2401 ttaactttat ttcatttttt ctcaatttat ttttatctta ttttattgat tttaaatatt
     2461 ttatttcatc ttctgttagg tatctatatt ctccaacttt ggtgtctttt aatactattt
     2521 tccccacaga aatccttctt aaatttaaaa ctggatgatt tatagctttg cacatttttc
     2581 ttacttgtct atttctaccc tcatgtatag ttatttcaca cactgaataa tttttttctt
     2641 tattttcttt agttaccctt attttggcag gtgccgttgt gtaatcttct atttttagtc
     2701 ctgtttcgaa ttgtcttatc tcatcatcac ttataattcc cttcacactt gccacataca
     2761 ttttatcaat ttcatgcttt ggatgggtaa gcttatatgt taaatcgcca tcattagtaa
     2821 gtattaataa accactagtt tcataatcta atctccctat tggatatact ctttccctta
     2881 catctttaag tatgtctatt acactgggtc tattgaattg gtcttttact gtagttatat
     2941 atccctctgg tttatttaat actatatata tataattttc acttggtttt acttttttat
     3001 tatcaaattc tactatgtct ttttcttcat caatattaaa agataactct tttactacac
     3061 ttccatttac ctttattctt cctccaagaa ttatttcttc tgcttttctt cttgatgcta
     3121 ttccacaaga agctatatac ttattaattc tcatacttaa tccttcctta tcttaaatta
     3181 tgtgtgttta atatttttat caatgttaat tttgctacat tattatacta tactagacct
     3241 taattaaaaa caagcaaaaa aattatttaa ttttcctaaa taattgtaat ttcttttcac
     3301 atactattaa tatgtaatag ctgactaaac actattttaa ggaagtgatt attatttcaa
     3361 aggaaaatat aaaatattat ttaatagtcg tgtttattgg aattttattt tttaaattta
     3421 ttaatactcc atcagacttt atatctagta ttgaaggatt cttaaagttt tttagcccat
     3481 ttttattagc tattttactt gctttacttt taaatccact tgtgatgctt tttgagataa
     3541 aattcaaagc acatagactt ttagccattt ttttatctta tatatttata ggttttattt
     3601 tagcctttgc cattaggtta ttaattccat caatagctaa tacattaaat agattgataa
     3661 atgaaatgcc tatgtatact gattatatag atagttttat agaaaaaaat atgtcaaaca
     3721 tatattttct aaaaacttta attcctcata tacaacatag tttagataat ttattaaaag
     3781 aagcaagtaa ttttgttggt aaaattccta aaaacttctt aatctataca cttagtatat
     3841 cttctatgct atttaatatg acaatgggat ttatactatc tatatacatt atatatgaca
     3901 aagaaaaaat tgcattggga tttaagagat ttctatattc ttctactgct agaaacaagg
     3961 cagataacat tatagaattt tttaggacta ctcatgatat tttctatgat tatttacttg
     4021 gtagaatatt agactctctt attattggta taattgcatt tttaggattt caatttgtta
     4081 tcagaataga aaatgcttta tttttagctt caataatatt cttaggtaat ataattccct
     4141 attttggtcc ttttattggt gcaatccctc caatagccat gactatatta tatagtccac
     4201 aaaaaactat atgggtcatt gcatttctat ttatattgca acaattagat ggaaatttta
     4261 tagaaccaaa agttatgggt aatcaagttg gtataggagc tatatgggta atatctgcta
     4321 ttttggtggg acaatctcta tttggtttca taggagtttt tctttcagta ccaatagctg
     4381 ctgttgtaaa aacttatgtt gataaatata tagataatcg tttacaatag attatctcta
     4441 ctgtgacttt aatttttagt ttaaaattta actgctattt attaaattat ttctcaaaaa
     4501 atatctctta gtaagaaaaa attgctaaga gatatttttt ataatatata atttgttcat
     4561 taaatttatg catatatttt atacttattg attatttgag ttttgagaat ttccaccact
     4621 atctgaacca ccagtagtat cttgtggagt tggtgctggt gttggattac tattatttgg
     4681 cttttcttga ttttgagaag aactgttatt attgtcagac ttattactat ctgatttttt
     4741 atcattgttt ttagaactag atttattatt tttactactt ccactataag aagatttcct
     4801 tgcatctgat tcaactctat acctatcttc tccagccgta tcttttgcct ttttctttct
     4861 ttgttcctct gcttcgtttt gttctttttc taatttagtt aactcttctt tctttttagt
     4921 tgcctcttct ttaataacag aggaaccatt tttgattttc tctataagtt taagttcctt
     4981 gattgcaccc tcataatttt ttaattcttc aaactccgtc atatttgaca ttctcatagc
     5041 ttgcatatac atagctcttg cattctcatt agtactatct tcttccagtg cagaggataa
     5101 tgattccata gcttttttat agttatgttt ttctaaatat gtttttcctt gctctactaa
     5161 atttatttca tcttttttat taaatgaaca tcctacaaga aatatagata atatcgatat
     5221 taataaaatt actcttcctc tcattaaatc cctccttgtc atatacttta aattttatca
     5281 taattataaa gtttttaaaa tattttatat ttattacaag aaatttgtta gtaaaacaaa
     5341 aaataagcag atatgctacg aacgatacaa taaaccaaaa attaatttag agatacatat
     5401 cctagcaaat ctgctcataa taaactttaa tatttaattt ttattatata cttctctatt
     5461 ttatatctct aaagctaata agtcctcata gctttgtctc ttacaaatta tcttatcatt
     5521 tccatcaaat actgatacca ctgctgctct tggtatttta ttataatttg aagacattga
     5581 atatccatat gcaccagttg atgttgttat aagtatatct ccacttttaa tatccattat
     5641 gtttatatct ccaattaata tatctccact ctcacaacat tttccagcta ttgttacatg
     5701 attcatagcc tcatgattaa ttttattcac aatactacac tcataactag cttggtacaa
     5761 cgaaggtctt acattatctg tcatacctcc atctacactt acataaaccc taacatcttt
     5821 tatatctttt atagaaccta ctgtatacaa tgtacttcct gcatttgcaa ctattgacct
     5881 tcctggttct attaccaatg taggtacttc tattccaagc tctttacatg aagtttctgc
     5941 tttagtgatt atggtttcac aaaactcttt tatagtttta ggtttatctc cttcgctata
     6001 ataaacgcca actcctccac ctaggtcaat ttctgttagt tgtatgttga atttttcttt
     6061 tatttctttt accaagttca tcattatatc cactgtatct atgtatggtt ctacatcaaa
     6121 tatttgtgaa cctatatgag cgtgaagacc tacaagttta atatttttat attcacttaa
     6181 tttttctatt gctctaaata aatctccatt tactaaagca aatccaaatt tagaatctat
     6241 ctgtccagtt tttatataat catgtgtatg agcttctata ccaggagtta ttctaaagta
     6301 tatctcttga gttttacctt tatcttcaca taatgcttca agcaaatcta attcataaaa
     6361 attatccact acaaatttac ctacacctaa atctaatccc atgtttattt cttctttagt
     6421 cttattgttt ccatgaaaga aaactttttc cataggaaaa tttgatttat aagctgtata
     6481 taactcgcct cctgaaacta catctaaaca catttgttct tcatttataa gattacacat
     6541 gtaaagaggt aaaaaagctt tccctgcata agcaacctta ttttttccct ctctagcctt
     6601 aaaatattct atatattctc tacaattttg tctaactaaa gcttcatcaa atacataaag
     6661 tggagtttga tattttttac taagttctaa acaactcact cctccaatat ataattcatc
     6721 attatttaca gtcatagttc catgaagttt cataatagcc tccccaaatt ttatatatta
     6781 aatacttctg ctattttttc tatagctact tgcttatcat cagaatttat agcacaagtt
     6841 atacgaatct ctgatgtagt tataagtttt atcattatac cattttcatt gaatatctta
     6901 aatactttag cagcaactcc agaagtattt ttcataccaa gacctactaa agaaaattta
     6961 gtgatattat tatcaatcac taaatgttct tcatctgtat atttagatac tatcttttta
     7021 cattctccca actcttcttt tggaactgta aatgatacac ttatcttatc taaaataggt
     7081 gcagtttggc ttatcatatc tacacttata cctatagttg ctatatcttc aaataagcta
     7141 gatatatttt cagctttaaa atcttttatt gttatagaac tgtcatcatc actagtagct
     7201 aatcctgtta ttactttatc ctctaatttc atgtcttttc cccctcttat atatgttcct
     7261 ttttctgtac cacatgtacg tccgacatag atttcaacac catatttttg tgcaagctct
     7321 atacttcttg aatgcattac ttgtgctcct aaactcgcta actcaagcat ttcttcatat
     7381 tcaatctcat caagtttact agcctttgaa tattttcttg ggtctgtaaa ataaattcca
     7441 tcaacatctg tatatatctc acattttcca tttaatttaa ccgcaagagc tacagcagat
     7501 gtatctgaac cacctcttcc aagagtggtt acatcaccat cttcgttgat tccctgaaat
     7561 ccagttacta tcacgacatt tccttcatct aaactatttt taattctgtt tacgtttata
     7621 tcatcaattt gagactttcc atgaagacca ctcgttttta tatttaactg gtaagcatta
     7681 taacttatag ctttacatcc aagtgcattt aatgctattg ataataaaga tgctgaaatc
     7741 atttcaccag tagacattaa agcatctaat tccctcttac taggttcatt actcaattcc
     7801 tttgctaaag ttatatattc atcagtggat tttcccatgg cagatacaac tattaccatt
     7861 tgtggatttt cttttcttcg ttctattata ttttctgcta ttgattttat tttttcagta
     7921 tctgctactg aacttccacc atatttttga acaattatac tcatgctttc tccccctcaa
     7981 gctaaatttt gataaataaa aacgcccaaa ggtatacttt ggacgtgtaa atttcataga
     8041 ccaagtaacc ttgtagctca ccactttaaa agtgacagcc ttatgcatat tctgcataag
     8101 tccagaaaat ttgtccgtca acaaaatttc ttcggctata taccctttca tcaattatca
     8161 acgcttacca gctcctattg aatactattg aatacagcta cctctaccct aggctcatta
     8221 tttttaatta atttattaat atagttttat catattctat ataatattgc aacaattatt
     8281 ttacaaaatg tggcaaatcg atagttttct tttctaaagt tagcttatat acaattaaat
     8341 agtttattta atcctaaatc tataaaattt atgctatact cattatcata tctctctaaa
     8401 tcatttaatt cggatagatt atctggtgta actaccaaaa cgtttggtac tttaaaaaat
     8461 ggaattgtag atttatctat ttttttagaa agattttcgt atttagaaat gtctatttca
     8521 ttatttaagt caacatcaac taaaacttgc tttatattac caaattggtc tgtatattca
     8581 ataaataaat cgtatttcac atcatctaca ctaacatttc ggtaaaatct ttttacatca
     8641 caacctgcag tattgattct aacaactaat tctgatccta ctagagcctt tattaaatca
     8701 tctcccttaa aaagtttact accgtttaaa tagtatacat aagagaggtc tttataatta
     8761 taataatcca catcacaagg atattttttt agtgtatatt cactacacat ttttctcaaa
     8821 gtttttttaa aatttttttt attgtatctt ttttggtctc cataaatttt tctaaattga
     8881 gactctgtaa cacatctaac cctgctaatt aaaagtaata tctctttttc catattcgtc
     8941 atagtacatt ctccttgaag tattatttgc aaagttgtac taatattata ttaattttat
     9001 gacattaaat ttacaaagaa ttaaaataaa atagctaatt tttatagaat taaattctat
     9061 aaaaattagc tatcactatg ttaaatacta ttatcaatct ttttatttaa attatacatt
     9121 accttgattt tcagaatctt tggaatctgt attttcatca gttcctgttt tttcttttgc
     9181 ttttttatct tcaaagtttg caatatttag gttatcaaat tctgacattt ctattacacc
     9241 agattcttct tttgaatacg atattttgac tttatcacca acctgagtca atggcaattc
     9301 gctagatact tttgaagtag ccctaaatat tatatcttta tttgaatcta aagttacata
     9361 ataattagtg ttaccacctc gcacatctgg gcttattctt gtcactgtgc cttctaatac
     9421 ctcatctttt aaatcattat caagttttaa atcattaccc ttagattcta atgcatcctt
     9481 atagtttctt aatgcttcat tcttatcttc acctattcca agtacattat aatcttcaac
     9541 agatacaaat gcaactctct taacaagacc cgccttatct tttaatgatg atacatatgt
     9601 tggctgaccc aatatattat acattacagg gaacgtcgct tcataatttt tttcttgaac
     9661 attaccttca gctgatttca ttgcagccgt ttcagtagct cctggttgtt tatataatct
     9721 agcttctttt gttctagaat caactaacat aaatccaatt gtagattcat ctccacctga
     9781 agatgttata cctgtatacc aataagacct gttatcattg ccataaacta atgatgtacc
     9841 ttctgttggt actaaaactc ccttttctga tataacagag tttaaaaatc cattaacata
     9901 aacaccccag tctttaattt ggtctgttac aaagctttgt ggttgtattc tatccaccca
     9961 tttaggcgtg tttttaacat cataaacatt tatttttcca gtttctgcat caacagtggc
    10021 tattcctgtt gcatttgctc ctccatagcc aattttatgt tcatataaac ttacaaccca
    10081 ataaggtctt ccttcatcat ttatttcaag agtgaaatct gtcataccaa catttacaat
    10141 accatgtatg tataaatgtc tttgtaaatc ctgatgtaag taggcttctg gttggtaaac
    10201 tatctttatt ggctttccat ctatttcttg aactaattgg acgtcttgag gattactagc
    10261 tgatactttt acataaccac ttgtaccact tagagatgtt atccatttaa ttatgtctct
    10321 gtgaacaaga ggcgctaccc agtacaactc tccatctaca ttttgtatat gaaattttcc
    10381 taatttggat acactcccta ttgcaggaac ttctccaagc tttttgtcac ctaatttcat
    10441 tgccatatct tcatctacaa gacgaatgtc atttacactg acagggctaa tgtcatcagt
    10501 aaatttactt tcagttactt ttcccaatag ttctctatat gattttgcat gaagtacagg
    10561 tgtagaagtt ataaagggaa ctactacaat atacacaagc aaagctattg ctattgaaaa
    10621 attatattta gccattttag atgctctttc atttctatct aacatcatat ctagtattcc
    10681 tgctacaaca aagaaaatta ttcctactga aaataaagat gtaaaatcta atcttagtac
    10741 aggtaattta ataaatgcat aaattattac aattattaat gcaactccat atgttttaag
    10801 atatcgtttc atttaatact cctctctaaa aaatgttatt attactatta tatccatata
    10861 tttacaaaaa ataaatattt atcatattta ttatatatta agatatcttt atatatttat
    10921 agttctgctt attcaactat atttaacatt atacgtgtat gtataaatac tttttataca
    10981 ctgtttgtgc tatccatccc gcacctaaag gagtgggatt tttgcactat tctgtattat
    11041 atatatccaa aaatgttata aaaatacaaa tattagcaga aatttaaaaa ctgaagaaag
    11101 tattctaact aagatttaca aattcttaat cctatcgaag ttaaactact atcttcagcc
    11161 taattatcta gcaatatttt tttatatgaa aaactctaaa tcaagttttt aaatgtttgc
    11221 gatttttgat acataagaat gttgtctaga attatattct gataataaac cttcatactc
    11281 taaggttaag ccagataaat tataaaaatc atttggcgtt acttcaaatg cttgtttatt
    11341 taaaatgtct atctcacttt tttctaacat gtcagcaaca aaatttgaac atacatattt
    11401 atattcagat tgtctctgaa tattcattag taaatatatt aatgccattg tatcgtaata
    11461 gtaatcttct ctataatcac taattaattt tatattttta tatagatttt catattgtaa
    11521 attatctact tctaacgaat ataccctaca cacagtgttt tttcttatag catataatcc
    11581 ttgatttata ttttcttcaa caaatcctcc aataaatgga ttccttggat ttagccttcc
    11641 aaaagaatac attggtttta gattttcatt taatgatatt gacacatgtg tatacagctt
    11701 tttacttata ttccttatta agtaagataa tatagttcct gtatatgttg ttacaatata
    11761 gactttattc atcatttccc cccaatctag taactaaata atattataca cttacaattt
    11821 aatatcataa gatattctaa cataataaat gtggttttac aaattttgat tttaaggtac
    11881 tttaatatta tataatataa taaaaatata aatataagtt ttctatattt ttaaccaaca
    11941 gatgttttct tcaaattaat cagatgattt agtatatctt gaacctttcc agagtccttt
    12001 aaatatccta atgccccatt tatttgtgat tttaaatcta tttctacttc catatccaaa
    12061 tcaacatttc ctttttttat agatatattt ttaagtgtta ttgggtaaag ataacttttt
    12121 tccaaagtaa tcttatttcc attaacctca aatggaactt ttttattgta gttacttaat
    12181 attttttcta gcattttatc acttagttta aattttccaa ttttcacatt ctcaagactt
    12241 acaactaagt ttccatcctt taaagtaggt ctcaattcaa gttcatattg actatttacc
    12301 aaaccaaata ccttatatgg tcctgctacc tttattttac catttttcat ttcaacaaaa
    12361 ttattttcaa gttcattaat tccatgcttc tgcatcaatg tatatattaa atttttaaac
    12421 tcttcctctg aaacagatat ttttccaacc attctaagtg gattttttac tacctccata
    12481 gaatttatat atgagccttt tgataaatat tcttcatcta tgattttatt attttgtgaa
    12541 ctaacatcat atctttcttt tggtgtcaag atatatacaa caattcctgc aagtactaaa
    12601 acaataacaa ttaaacttaa caaaatcttt gcaacctttc ccataatcgt ctcctttaca
    12661 aatctgtcat cagatttaaa ataaatttag ataactaact tcacataata ttcttaacaa
    12721 tatacatact ataatacagt cttttaaaaa ttaattcaac caatctaatg ttttgtaaat
    12781 aaactgtaaa aacatgtttt tagtataaaa gttaatattt tgcataatac tcttaattac
    12841 acctttattt ttttctaatc ttggattaaa attaatatta tgcaatacag tgtagtgttc
    12901 tgtattataa tcaaattaaa taggaggtat agaatatgtc aaatgaaacg aactcatcta
    12961 actttataaa gaatattatt ataaacgact tagaaacagg aaaacatgac agtatcataa
    13021 ctcgttttcc acctgagcca aacggatatt tacatattgg tcatgcaaaa agtatatgtc
    13081 ttaattttgg attagctaag gagtttaatg gaaaagctaa tttaagattt gatgatacaa
    13141 acccattaaa agaggatgtt gaatatgtag agtccataaa agaagatgta aagtggttag
    13201 gatttgattg gaacgagtta aattttgcat ctaactactt tgatgaaatg tataagagag
    13261 ctttaatact aataaaaaag ggtaaagctt atgtatgtga tttgacacaa gaggaaatga
    13321 gagaatatcg tggtactcta actgaaccag gaaaagaaag tcctcataga aacagaacta
    13381 ttgaagaaaa ccttgattta tttgaaagaa tgaaaaatgg tgaatttaaa gatggtgaaa
    13441 aaacattaag agccaaaata gatatgtctt ctccaaatat aaaccttaga gacccaatta
    13501 tatatagaat atctcattca acacaccata atacaggaga taaatggtgc atatatccta
    13561 tgtatgcttt tgctcatcca attgaagatg caattgaagg aataactcac tctatatgta
    13621 ctttagagtt tgaagaccaa agacctcttt atgattggtt tgttaaagaa tgtgagatgg
    13681 aaaatatacc tagacaaata gaatttgcta gacttaatat aaataatact gtcatgagta
    13741 aaaggaaatt aaaacaactt gtagatgagg gtattgttga tggttgggat gaccctcgtg
    13801 ttcctacaat atccggaatc agacgtaagg gatatactgc agaagcattg cgcaatttct
    13861 gtagtgaaat aggggtatca aaagttaact ctacggtaga tagtcaaatg cttgattatt
    13921 tcctaagaga aaacttacaa ccaaaggctc ctcttactat gggagtttta agacctttaa
    13981 aacttattat aactaattac ccagaagata agattgagat gcttgaaata gagaataatg
    14041 ctaaagatga atctcaagga aagagattag ttcctttctc tagagaattg tatatagaac
    14101 aagatgattt tatggaagaa cctgttaaga aatattttag atttttccca ggaaatgaag
    14161 tccgtttaaa aggtgcttat tttgtaaaat gtactgatgt aattaaggat gaaaatggaa
    14221 atgtagttga aatacatggt acttatgacc ctgaaactaa gagtggctct ggctttacag
    14281 gacgtaaggt aaaatctaca atacattggg ttgatgctaa atcagctata ccatgtgaat
    14341 ttagattatt tgaaccatta attcttgatg atatacctga aaatgaaggt aaacatttct
    14401 taaaacaaat aaatcctaat tcattggaaa tattacaagg ttttgttgaa cctacacaga
    14461 taaaagatgc taaacctttt gataaattcc aatttgttag aaatggattc tttagtatag
    14521 ataataaata tacaactgat gaaaaatttg tatttaatag aatagttcct ctaaaaagtt
    14581 cttttaaacc aggtaaataa tcttacaatc aaaaaatgaa gacttaaaat ataaattact
    14641 tctactataa ataaacttag ttagaagtaa ttttttattt gagtcttcat catttacttt
    14701 aaatgattaa cttttttcac tacagattcc catgacttct attccatctt ttatgcattc
    14761 tatgttaaga ggaaaatctg gtcctttttc tccgtcaata tctgttataa gtgaatcttc
    14821 acattctata tgcacctttt tagttttaaa gtatagtatt tcatttccat tatattgatc
    14881 taaataatca cctctaagca cacttatcaa aactggtatt gattttggta ttggcattcc
    14941 tttaaaaata attacatcca aatatccatc atctatttca gccttatatg ctagatttat
    15001 atttcctgct gttttgccat taaaaatcaa cataagatac atatctccat cataatacat
    15061 ttcctcagaa gatactttta tcttaaattt cctcatatac aatgcttctt ctattccctt
    15121 tatataataa gaaattcttc caaatgaatt ttttaagtct gtgtttatcc tttgtgatac
    15181 atccgtaaac ataccagcac ttgcaacatt tataaagtac ttattattga ctttccctat
    15241 gtctattttc tttggttttg aatttatgat ttcatttata gcttctttta tatcaaatga
    15301 aatttgaaga gcatttgaaa aatcatttgc agttcctgta ggcaaaattc caattggaat
    15361 ttttatgtct aattctttca ttgcatttac tactaaatct acagtaccat caccaccagc
    15421 tatcaatatg tgttcataag aactatcaac ttccttaaat gcattcttta caggttcttt
    15481 aatagttaat ctatagggta tcaataaata atttttttct tgatataatt ctatgatagt
    15541 gtctaaattt gataatatct ttttttcacc tgaattagga ttgtatataa gttttacttt
    15601 tttcattatt tctccttact cattttattc ttttagtatc caaaatcttc atttaaaaaa
    15661 atcacatgta ttctttctgg tgttaattgt ctttttataa gtgtataact acagcatatt
    15721 cttcctggac ttgaacagtc tatacatata ccagtagtct tacatccagt atttgtactt
    15781 aatcttttac aattggctgg agcagctatt gtttttgttc tttttatagc ttcatctaca
    15841 tctttaacta ttttatttac tcctgctata actattactt tatcaggtcc atatatcata
    15901 gcagcaactc tattcccatt cccatctaaa ttgtaaagtt ctccatcctc tgttatcgca
    15961 ttagtacttg taaaataagc atctgcagaa aatgactttc tatatatttc tttcatttct
    16021 tctggtttga tatcttttgc atatctatca agaaaattat atttttctct tataaaatca
    16081 atcactcctg tttcaaataa ggtcatagaa cctccacatc ctactagaga acctgcttta
    16141 actatttctt caattttttt aagaagctct tctttgttac ttactaaata ccctttcata
    16201 ttattgtttt ccaaagattt tattgttttt tctattcttt tttcattaag ccaatttaaa
    16261 ttttgatcca ttttaacttc ccccttattt ttaatatgca cttaattatt tataaacttt
    16321 attttcttta taagtttatt ttagtacatc taaaaaaaaa tacaatataa aacttaaata
    16381 gttttacata attttttcca aaataactta ctcaatcaaa aaaggaaagt agatgtacta
    16441 ttctactttc cctatattgt atgtagtatt tattgcctaa aatctatata ttaattatat
    16501 gtttcatatt atataatatc acagttaatt aaaatgtaat actatcttct ttttaaacat
    16561 ataaattgta ttattaaatt taatatatcc aacttcaata aatcttatta atttgatgaa
    16621 gaagatgcta tagctgcact tgttgctgcc attacacatg caatttgaat agcaacaaca
    16681 atatttaaaa atatattatt agttatagag cctatattat tctcatttcg tatcaaatct
    16741 gcataaattg aagctacaat agcagaacta atcattactc tattactttt tcctaaagac
    16801 caatttccaa agcctttttc ttctttaagt ttatttgaaa cattttttat ctcttctatt
    16861 atttcatctt ctctataatc taataatgaa attgctccta ttattggata tccataataa
    16921 ttcattttac aatcattttc tttaaatgct tttttaagtt caatagtctt tttacattta
    16981 agcatagttc tatcatcact aaataaaaga atttgggata atgcttgtaa atcatttcct
    17041 ttataaaaac cattttcatt tagaaattca taacattttt ctatgtattc taaatcttca
    17101 tctaaatttt ttgaatgaat tgcaataagt gctatattac aatagtcatc actagaagtt
    17161 agaaatggat gttttttcct catgtaatca tatattattt tcatttttga tatacatgta
    17221 agataatcca ttttctctct attttcataa actattgatg ctgtaagtgg taaatatgta
    17281 tcgccccaaa acttttcact tttaagttct ttgtatatat ctaaaatcat cttaaaacta
    17341 ctttctggat ttgattcaaa cgaaagtagt gttgccatat aaaacatatt atatcctcta
    17401 aaattagaga ataatccagt atttttttta ataagctcta ttcgttcttc cactaatcta
    17461 ggacttattc tcttgttctt aagggtaaat gcaagtgcac aactatgttg tatcaatccc
    17521 atttgccaag aacctttaac ttcatttaat gctttgtaat tttctattaa taagtttact
    17581 tttccattta taaacgattg ttccatattg ccctcctagt aatatatctc attgtactaa
    17641 tagttttata tttattatat atatattaaa tactacaatc aatacatatt acttcaaaga
    17701 acatcattct tcaacaataa tgtagccttt tttgtaatgt cttcccatat tattgactct
    17761 atcatagtac tctgattcac tttcattact ccatttgggc acaaatacat ctattgcatt
    17821 agaattacta aaatacttac tagaccccct atccataaca gtttttaatt ttccatctac
    17881 aataatctta gttccaaaac ttaagtgatt gtttgctaca aaaccatctt ctaaatcatc
    17941 tcctgtagca gttatcgctc catatccatt ttcttgatta agacttgtgt aataactaac
    18001 ttcaaaagtt ataggtacta acttctttat tttaatacct aactgatttt gtagtttctc
    18061 cttttgtact ttcaacttat actcatcact atttttataa tcttccatac cttgattata
    18121 cttggaatta aagcttttac ctgactttac tatagtatct atatttacta tagattcttt
    18181 acaatgatct gcatctattt ttgtttctcc atttaccaat aatggagtta aacatacact
    18241 acatacaaca catgtaatca atgcttcttt aagcttcata aattcccccc ttattatttt
    18301 gatatattag catatttaat attatatgtc tacatttaga tttaaatacc acacccattt
    18361 gctttaagaa gttgtcttat atttaattca ccttaaacac ttataaatcc tctatcttag
    18421 aatcatatag aataaagctt ttatctttaa atatttttta ttaatctttt aaaatctaaa
    18481 actactttat ctaattttct cttttttaga atttaagaat taaaattcta taattttaaa
    18541 acatattgaa tttatttttt tgagatgata taattttctt aagaggtggt tgatatgata
    18601 aaaaatacaa aacaaattac agtagaagtt aatgaaattt tattacaaga aatgtctaag
    18661 ataactaatg acgaaaataa tattttgaca gatttgataa atgaatcaat tgtaaattat
    18721 attcttgaaa acaaaaaaca taatattgaa aataaagatt tagaagaagt gattaaattt
    18781 tacaaaaaag aaaatactga aaaacctaaa agtgcagcaa gagctatagc tgaagcaaaa
    18841 actcatactc tgtattaatc aaataaaaaa gttcctaaaa actaggaact tttttatatt
    18901 aataattatt aatatatctc aatatttatc tcaaatctat caatataagc atgtttaata
    18961 caatctattg tatacttatc ttaccctaga aataatcaaa acaaaaataa aattatgccc
    19021 tttatatcta aaacaattaa tttacatgat taatatacaa aaattctgac ttttaaattc
    19081 gtatacaata tcaaactaaa ataatattaa tgacaaaccc aatataaatt aaattcaaac
    19141 aggtttgatt ttacatcaaa cttattgtat taatattaaa taacaagaca aaaatattta
    19201 taaaaatcac aaattttaca taaaacttta tatttgattc atttaactaa ttattgtaaa
    19261 aactatttat tttatgataa ttaactacac caaattattc atctttttta atatccttaa
    19321 cccatttttc aacagagttc aattcttctt catttacccc ctttttgaac aaggatttca
    19381 taaaactctc aatagaactt ccatgcatat tatctaaaaa agtttttgtt tcaaaatgta
    19441 aatactcttc ttctccaata agaactgtat aatgtgtatg tctatcaatt tttcttgcac
    19501 ttaagaattg cttatttact aatcttgata aaagtgttag tgttgtagtt tgcttccaat
    19561 tatatagttt ttccatagct tcaatgacat cttttgatgc tactgtaaaa tctgaactcc
    19621 aaataaattt catgactttt aattctgctg gtggtatctt agtaatagtc atatgatttt
    19681 acactcctct caaacttcta cataattaaa tactatatta atagactttt cgagtcaaag
    19741 gttttattta aaatatctca tccaaattta ttaatacaaa aaatttatat ttttattcaa
    19801 aattaaacac acaatctatt aatataaaga taaatatcta ttctataagt ataacagatt
    19861 atcaaattaa aattctaata tttgacataa tatatacatt ttttaaataa atatatacaa
    19921 tgtattaaaa tgtatgcaac tctatttcaa aattattttg tgatatatat atgttataat
    19981 aaatgtataa aattttgatt ggtgggttat atatgaatgc atttattaga aaaagaaata
    20041 aaaactatgt tgtttacctg gaatttgaag atgaaaagac aggtaaaaga aaacaaaaaa
    20101 atatgggtac ttttgataaa aaaagggaag caagtaaaag actaaatgag gtaaaagaaa
    20161 gcttatataa tgatggtttt ttagtaccaa gcgaaattac agtgtcagaa tttctattag
    20221 attttctaaa taaatatagt gaaaatatat ctttagctac atataataat tatgttagta
    20281 tttgtaagaa ttacattaat ccatctattg gaaaatataa aatacaagac ttacatccgg
    20341 ttcacataca aaactacata gataaactat cttataaact aaatcctcaa tcaattaaga
    20401 ttcatataaa tatattgaaa cttgcaataa aaagagctta tagactcaaa ttaataaaag
    20461 aaaatgttat ggatagtata gaggctccaa gatataaaaa atttaaaaat gagatttatg
    20521 atagagaaca aatgataaaa ttattagatt tagcaaaaaa tacagaaatg gaattaccta
    20581 ttaatttggc tattggatta ggtcttagaa tttcagaagt tttaggtcta acttgggata
    20641 atattgattt tgaagaaaat acaataacag taaataagat aacttcccga attaatggtt
    20701 ctgttattct caaagagcca aagacagaaa gctctgttag aaaaatatct tctcccaaag
    20761 aattaatgtc tttattaaaa gaatataaaa taaaacagaa taaaaattta ttaaaatctt
    20821 ccattagaaa taataataat ttgttatttt tcaataagaa atgtgaacct attgctgaag
    20881 atgttatgag taaaaaattt aaaagatttt tagaaaagaa tgaattacct catattagat
    20941 tccatgattt aagacactca catgttactt tacttataaa ttctaaagta cctataaagg
    21001 ttatatccga gagagtagga cactcaaata taagtactac tctaagcgtt tattctcatg
    21061 tacttaaaga aatggataaa gaggcttctg acaaaatttc tgaaagttta tttaatgcta
    21121 attaatatta atttatctta gctttaaatt agcttagctt attgagatag attcattaat
    21181 attaaatgta tctatctcaa attaataact acttaaataa tctgttttat tttaatttat
    21241 cgcctcagtt attatacttt tttacgaaaa attatacttt acaataaaaa agatattccc
    21301 ttatagaaaa tatcttttta aatactatat tattatatac ttgctaacat tggaatatct
    21361 aacatcttct tcattacatc atctactttt tttgctttat attcattgta tccaattaaa
    21421 tatccttttt catatgaact ttttaaaatc tctttttcat ataaattatc agatttggct
    21481 tctgtgacac ttaaattata aagcccttta cacccatctt cataaccttt attataccct
    21541 ttttctgcaa aatagttttt atgatttttt tcgttatgta ctgaagatat tgttactcca
    21601 gaattttctt ttgtactagc ataaggacaa aaacctttat tgtgttgatg tgctgaatgt
    21661 cctccacaat aataacgtgt taactctgat ttatttttat tatgatgacc tcctgatgaa
    21721 ttatgccctc ctgagtgagc atatgaaaaa ttccctacac taatcatagc taaactaatt
    21781 actacagcta ctaacctttt cttaaccata atctattccc cccaaaatgt tatatatttt
    21841 tattatatca tctactatat tatatttaaa ttcttaatat acaaaatatt actctattct
    21901 gctaataaac atgtttaaac aatataaaaa acctcctaat atttgattaa aaatttacac
    21961 actcatgcat agcatgtctc aaatattaaa aggttttata ctaataatta tttatctttt
    22021 ccatacattt tagctaattg gtcttgtatc tctttacttt ctaaatactc atcaaagtcc
    22081 atttttctat ccattattcc atttggagtt atctctataa ttctatttgc tatagtagat
    22141 ataaattcat ggtcatgaga agtaaataaa agcactcctg ggaacttttc aagtccttta
    22201 tttacagaag ttatactttc taggtctaag tggttagtag ggtcatctaa tacaagtaca
    22261 tttgcatttg aaagcattag cttactaagc atacatctaa ccttttctcc tccagatata
    22321 acttgagctt cttttaatgc ttcttcacct gagaataaca ttctacctaa aaatcctctt
    22381 ataaaacttt cacttttttc ttcagagaac tgtctaagcc agtcaaccaa agaatattct
    22441 actccgtcaa aaaacttatt atgatttttt ggaagataat cttgagaagt agttactccc
    22501 catttatact ctccactgtc tggttcaagt tctcccataa ctatgtttaa aagtgcagta
    22561 gtagctattt catctcccat aaatactact ttatcatctc tatccaatct aaatgacaca
    22621 ttatcaagaa ccttaacccc atctatagtt ttagttaaat tgtgtacttc taaaacttca
    22681 ttcccaattt ctcttgcagg tgtaaatcct acatatggat atcttcttct tgatggttgt
    22741 atatcttcaa cttctaattt ctctaactgt ttctttcttg atgttgcttg tttagccttt
    22801 gaagcattag aactaaatct agctataaat tcctttaatt gagctatctt ttcttcagtc
    22861 tttttatttt ggtccttagc caattgtaaa gccaattgac ttgactcata ccagaaatca
    22921 tagtttccaa cgtacatttg tattttacca aaatctacgt caactatatt agtacatatt
    22981 tgatttagga aatgtctatc atgtgatact attataacta tagattcttc taaatccatt
    23041 ataaaattat ttaaccagtt tattgattta aaatctaagt ggtttgtagg ttcgtccatt
    23101 aatagtattt caggcttacc aaaaagtgat tgtgctaaca ataccttaac cttttcccca
    23161 ccaactagtt ccttcatttg tttgtaatgc atatctttcg ttatgccaag acccataagt
    23221 attttctcag cattagtttc tgcatcccat ccgtcaagct ctgcaaactc accttcaagt
    23281 tcggcagctt ttatcccatc ttcttcatta aagtcttcct tcatatacaa tgcatctttt
    23341 tctttcatta tgttccaaag tctctcatga cctcttataa caacatttaa aacagtttct
    23401 tcttcatatt caaagtggtc ctgttttaaa actgacattc tttctttatc agttatagat
    23461 atactacctg tattaggctc tatttctcct gataatattt ttaaaaaagt agattttccc
    23521 gcaccattag ctcctattat tccatagcaa ttacctttag tgaattttaa gttaacatct
    23581 ttaaatagtt ccttatcacc aaatctaagt ccaacacctg taacttgtaa cattaatata
    23641 ttttcctttc tttttgctca taatttattt tatcataaaa ctttgataac atttttatta
    23701 tatattatca cg
//
LOCUS       DAMJVK010000061        23656 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.062, whole genome shotgun sequence.
ACCESSION   DAMJVK010000061 DAMJVK010000000
VERSION     DAMJVK010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23656
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.062"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(43..582)
                     /locus_tag="PC414_002440"
     CDS             complement(43..582)
                     /locus_tag="PC414_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464828.1"
                     /translation="MLNEDVVKKLKNVPNNTNTEIEKVNTNIETAKTELNTKIDQLIA
                     GGSNVASTQTITIDDWVDDTENGFKATVTHGLLTQRIVVNIIDATTKENVVTNFKIID
                     DNSIEIRSETRSELNVYVINGNAETHFINATVDDNRVSEMTTYSSKKIEDRFVNLEEK
                     VNGGLSNIATSVNELITYC"
     gene            complement(662..985)
                     /locus_tag="PC414_002441"
     CDS             complement(662..985)
                     /locus_tag="PC414_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464829.1"
                     /translation="MIKIDKEKIEKSRQRKELDDLDSSQKTQDNDITDLMLATAEICE
                     MVLSSQQTSTMSLKNIKLNEGGSSMAAIYVGLIERGLKTIDQVPVKYREEVRKMCEAL
                     EISLS"
     gene            complement(997..6276)
                     /locus_tag="PC414_002442"
     CDS             complement(997..6276)
                     /locus_tag="PC414_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464830.1"
                     /translation="MKKNKLLQRGSLFGNKEFKIEEFNKNFDTFNVKDIFTDMDSGSV
                     FIIKDDLKLYAAGYNVFGNLGLGNKVSPIRYFSKIDIDNVKYVSSSGTHSLLLKNNGD
                     VYSTGLNTDGELGLGDNIDRNTFTKINISNVKQIACGNGYSMLVTNDNELYVCGNNKF
                     GGIGLGNTSTVNTFTKIDNLSVKEIFAGVSCSYILTLNNELFSTGLNNYGQLGLNDTT
                     NRNIFTKVNLDNVIKISTAQGAVNFLLENGDVYGCGDNSSGQVGVGGGATVTVLTKLS
                     ISNVKNIESGRYHKFYIANNNDIYVTGANPDGRLGDKINCDRSTYTPRKSSISIKNIS
                     KIKCAHAISFILTSNNELYMTGDNNYSFSYLKRYDKFIYGFEKFEDFDIEECNHIESI
                     DNRLLINKNDERVLDIGLENNFVNISLVKANDTSTFIVVNGNELYAAGYNAYGGLGLG
                     HNRHCYEFTRVPLELEEGVTIKDIYLNLGSYFTFILLSDNTLYSTGYNVFGVLGLGDN
                     INRNTFTKVNISSVDKISVGDNHALLKTTTNELFSTGLNNYGQLGLNDNTNRNIFTKV
                     STVTYVNEIIAYSNSSYCIDNSRISYAAGYNVFGELSLNDTSSRSVFVKMKYLRYKTD
                     TTVSDVSNIAEIIPFNNGCLIRTTSNNYYVSGRHNYYSTSIKNSVTDDYYSRFGEFVG
                     SKNSNANEVAVLIDEPKLSKVLVSNYNLCLLLDASDGGYKEIKFTGRPSTFGASSQDK
                     NLGLRSLISSSMPYYNHYLGTDNLLYTTSKTRMESKFEIRGRNAGGQRGDSSYYSNSQ
                     KYTLSNAFFAYNSQVIYASRLRIVIKDYFGNIYCSGVNSHGCFGIKKPYDVRHDGFVN
                     ISKQFSKKYIISNIKEIKSDRQALYILLNNSEGYVCGNNAGNKLGTIKARSEFIKVPI
                     DNIKEFIPSNDCLFILTNNNQVYSIGTNDLGQLGLGDNIDRGTFTKVPIDNVKKVITC
                     YKSSYILTYDNELFSTGSNGYGQLGLGDNINRNTFTKVNISGTIKDIFCGRYVFIILL
                     ENDNIISLMGCGNRNYISSNTNSNSLTKINIEGLTDPSTISNLMLEEGKTIITMKNRI
                     FVTGYNSLGGLGVGTSYNNSSGYIEGYKDLAFNSNIKEVALGEYTIFVLLENGDLYSG
                     GNADDGIGFNESGTVVLKKLTSNIKHIYANGKNAYYIDNDNALYVFGSRQLGQINNYS
                     DVVQKTPVRNCLNVKQVYTQHNGLIVQYTNNKLEGKGNAVVGQVGIFNGHEENQIANT
                     SGDNPSDLSFSNEMSIIRNNYGLLVNKKIFLHNDDSINYRIPFGTNAGNYSTSLPFDN
                     IKKMSLSSTHSILILENGDVYGCGSNQFGELAINKSELTTTSEFIKINLTNIIDIACG
                     DNFTYFLKNDGSLFSIGKNSEYQLGIGHNDEVNELQEVLTISNIREIYAYSNYILAIT
                     TEGQLYVQGYNINGILGLNENTKNTIIKLFTKVLENVKYIKSYDDKHIFVIKNDKTLY
                     VTGINKETYKIKDVEIGSMLYTFSQIYIPRDVNDIVDALIKDETLYIISKVDSEKTCL
                     EIKNKSYSSIEIDLQNPNNELTKIEMFINNISSSIIEDLSTGTVTFEINPENLVLGEN
                     KIVFKAHSDIGNNLYINVYIYKKETGATIVKDSTVLIDGNTYNVSSIVDNSQDVVLTL
                     NKGLLEDLNSNNPIYHLVNKLKVQLKINESDTFKDMVKVETRKTENGYKEIYELKDMN
                     IQSAQPKVIVEEGNTNTTIKKPSMLFNLDVETL"
     gene            complement(6287..8365)
                     /locus_tag="PC414_002443"
     CDS             complement(6287..8365)
                     /locus_tag="PC414_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDF2464831.1"
                     /translation="MKNYEIKNHTIEVDFSNYRINKRLLDYFIYNENDVKTAYIEAIL
                     KNKDEIIDLSEYDRVLVSITKSDGQKVNGECEVVDAENGIVEIELSRQALASVGINTF
                     QLSLVKDGTLLNTTNLYYRVEEGMINDDDITSTDEYGVLLVIIAQAEEIIKNNKELTK
                     RVEQLEITILGNEEVRDKAEQIRIYNEDIRNIQEEEREFNELTRQNQEANREESIQNM
                     QIQVDDKLTDCQLQLDEMIDAKSKEIDNIVDDKMLDVQVQTDKKFQDLDTRANNTFDL
                     YDKTFEDKLTDNQEQIDYKLDEVNQAISNVEDCIDESTTKLDTKIKEVDDKIVEVNTA
                     KTDMTTTVSNKITEFENRFEELESLDARGELIQARESVDGTVKDTLKDRLTYDFEKVN
                     EKIAEMTSAATNVAFSKSYVESDWVADGEYFKLIVNHNLVTENIFVAILDEATKKSMT
                     NSYTTVDSNTIEIFNESNIDVKVTVVNGNTNKEVIQATINDNITTLDSTYSSIKIDAK
                     FDESLTKINENKSNIATNLEKINLIQSKVGSSELSTVSKNISDAVNELDASVKVLESG
                     GNTNEQLKILKENYNKMSVEILKILFELELDKEATADEAGYWYDTLYDSKNISNLDGL
                     KLNKFKQQLELLSNSGTVLFKNVTVPFLCNKIRYIHDLGSNYSETKTEESYQKGDTEI
                     KINKYSYEVR"
     gene            complement(8377..8532)
                     /locus_tag="PC414_002444"
     CDS             complement(8377..8532)
                     /locus_tag="PC414_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464832.1"
                     /translation="MEVNLQKAYTVAFEEIKSLYNELILYKALNMQQQEEIENLKKEL
                     EEQNKEQ"
     gene            complement(8534..10456)
                     /locus_tag="PC414_002445"
     CDS             complement(8534..10456)
                     /locus_tag="PC414_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HDF2464833.1"
                     /translation="MILDNSPADSWIRITGVIVTYNNTLYQVVDTETNKKYIYWDADN
                     PGTLKVSNVRLPEGNTQFLVVVNDNGKHTEVPINSSIFDISFDGNSRKNTEEQIWGLY
                     ETDEKHNEKFVVIEKDIDGIHQTVLEVQEDASHIKENMSLIDQRAENVNILVKEVTKN
                     FGGSQENITLRENINKAIIKLNADLGTFSSNMSNYFNDNEVTDEEKEKIDIELNLLDT
                     DKASLYTELQKLIDRTTGVDLVAINTSKTALDAASTNLNSIINSVISDSIITPSDRIL
                     AINANAQYNLKINELKNTVDKIYITGMGGSISEEFSQINATAKEIKLEVAKVDGATKT
                     NAAEIKLTKDDITMMVTRNNSGSIVGIKPDKIEFGFNDISNYVEISRSGLTVNQGAIA
                     CDILTTPSGHEPIIRLFGSSRSGFAIDARQSDGSSQASAIRLKYDSNNYFWVGYDTAE
                     IYVDGEEHFIVERDDTFVRCGGATFTFTNGDSLGVGYSFYPERSVTDLGCERYKWRYL
                     YARSTLSESDKKFKENIVYIKDIKNRTRSSITPTPFLDFIRDDFKPATFDYIAEKDRT
                     IADSQIGFIANDFKDSYVGKTFLYDYGEENGLMFSPSGYTTVVATALQEEIQKREELE
                     MIINELNEKINNLGGY"
     gene            complement(10458..12173)
                     /locus_tag="PC414_002446"
     CDS             complement(10458..12173)
                     /locus_tag="PC414_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464834.1"
                     /translation="MHFNVNLKQIKSDYTLTIHKMNKSFLGQIPINFLNSIKRELGGV
                     DEIQLTIPKYITDRFLFNKIINPIFEEVKEERLICLNNKEYFVVKNVVTTDDKLKVVT
                     AKSKEVKLGKIDVNIEDYGLQMFTKDEEASIISLNDYLKQETGWKLGYVDDSIAYETD
                     SEGNKREKVRWQESINSNWLDYFNNELKEQFECIADFDTYNNLVNLYHIDSFGDNIQL
                     YLSHDNYIKSLERTTNSDDIVTRLKLEGSEDMDIIGATVTGYDYIENYSYFLDNKEMS
                     EELSRAIKKYQEMNEIREPIWRELIDTKLKKQRERDSKSNEWLHVIETIKAKKDIKKT
                     YDNPEHKDEVNSAKLAVEISELEDKKVILDVQIKHLEEEIAKLDESIKDINILCKRET
                     STDEDGYLIFNEALLDELNEFLYYDTYTNDAFLKVEDLIAEGKRQLSLKCIPTKEWTL
                     DVINFLDRIIDINFRQHWKGDLSLGDIIVLHSKESKEEELVYFTSFTQNFKNGKLDTL
                     ELTLSNKKVKEDDKRTIADYLTKAEHATRTLNSKRHLFIQQQKKRINLPDEYIPKKNI
                     QKELM"
     gene            complement(12526..12681)
                     /locus_tag="PC414_002447"
     CDS             complement(12526..12681)
                     /locus_tag="PC414_002447"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464835.1"
                     /translation="MINNIEDCKLLIEAEIRMKDIDEKELISQRDILKDLNINEKDLE
                     DIEVNFE"
     gene            complement(13831..19905)
                     /locus_tag="PC414_002448"
     CDS             complement(13831..19905)
                     /locus_tag="PC414_002448"
                     /inference="COORDINATES: protein motif:HMM:TIGR01760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDF2464836.1"
                     /translation="MEDKRKRGGSVLHEYGVSLGDTVGMITATNEAIQNPEKVGNGLK
                     SVAINLAGMKVSADDGTLSLNKTAKGLKEIAGITIYKNKDTGELKNMVQLLDELKGKW
                     GELREDEQKGLAEAIAGKQQAAIFQSLMGNYETFKEIRSEFSQGLQFGSAEAENKQYL
                     DSLAGRLNELKTIWADIFQTVFNSNTTYGFLDGLISVSNGINSVIKTLERLKLTTPAL
                     IGSFMTLKTLFSGMGMTNGTRNVARLINPLNMIGTPIKATTTILQSFRHAVMAANVAG
                     ANGANVITRAGTGLGALRQSLSRTSIGIGTLNIATSLLNGAMIGLATFAITKGIQWID
                     DTIHKYEKLAEKQKEVIGTTTSEISSLQQKKKSLEGISEEYNNLASKTEKTADETERF
                     SELKQQIADIAPELVLGVDENNDPILNMSGNLDEVINKLRKAIEEKNKLLKSQEDELA
                     ITTSKTAPDTKKDLDKEKKKLDKVLEDEHNLFKNALSKSNFKEQTEALKKAYNQQETD
                     FAEHHQKLLTEYEKYNEEEEVMQKRAINRLTNSDISKTYAKQSEQKKSKMNELVANMN
                     WHDYSVGQQNSIISAFDKISESIPVEKLDQWNKKITDANINYQASGDMNQYTKELQGL
                     ANELQKLSGGSISADTWLIGLQQGFSGLDLASMKLHTFLKSYGSSLAELENKDSFAIK
                     LKNQFDALNDFSQDLEVNGKVNIKLLEDIQSGEAGQDLPQQLKDIINGFTFGEDSQIE
                     AQAQLALQVTTTLQNEGQLSNDVVKQLNDLFKGKLQLTDDILKNGLKISDKLPALPGN
                     MVQALNEQFANAFTGDNAPEITPSVNGEKAKKELKELEKFKKDTFKSDKIVTEINNKG
                     LDFAKKDLSVLKEQLDRFKNEDTKLSFTTDCADAFKDVNSLEEARKKISAPMSLKYDI
                     DVDKKGLTEAKKVYDILPDNLKKKIETEVIGIDKLDTLQNLIDKAPNKKETIFEIINK
                     NPDKLNEIDEILNNVPNDKKEAVIDFIVNNLDQLEKAKNVQEFINNLPPEIKKQLGIE
                     VEGGENAEKTVNTVQNADGTKADLLVNAEVNGESQVVECIETLDKNGNKTYTPNLNPQ
                     VNNSEKVVQCMRILDEYGNESYIPIVSPQVTNEEGLNSTKQKMDELDNKTVTPTVQIK
                     TEGETEVEQATNFIENTNPTKVIELSINNSGKDEIDNTKQKLDELNNEKATPTIEATG
                     AEQASEQLNQLSQKAQEIGQGNYHINVSTSTEQGAKNISGLIARVKQFLALKVSTLVF
                     RTETAQAAKNVSGLLAKVQSYVSRYGGKTIKTTFSALTATAAKNISGLIRKIESFKSN
                     YAGKTFTTTFVTNKVTNSSGGGGGGNSSDKTNKTERTSVTNGTPIPANLSQPRTSEPA
                     PISDETPVTKPSLFSRAVSRATSAFKVIKPKVTINNDTIDSSVKYSIELFKELENAIS
                     AVTNEIALLDKKMEHATGDEKIKYLQEQNKLFAQQKDLIKQQQDGYKAESNRLKNRLK
                     EKKMGFKFDDTGNLTNYEEKLISIEKELDKLNSKSGDKENKKDESRKKELEETKKVLE
                     EYIKVTFTELPKCEQEWEEYNNKIKEHNEELRKAQEEMWKLNKNSTWTSMYKDVEQVN
                     NEIAMIDVLLKNATGDEQIELLEKKKELMAKKAKELSETTAYLQETQKELQQKLQGYG
                     FTFRDNGDITNFIQHKNQLKKNMTDEEFKRMEDAAEGYLDLLINKIPDANKEIAESKK
                     EIEALNKEIQDAYKDQLKEAQSLQEKIRDMYKKELEERLKEIDKETKAKIDSLKKQQD
                     AYNDSRKEAKYKDDYEEQQDVISDLEKQIAIAERDSSLSGQKKLKDLQKQLKEEQKKL
                     QDLVQDHVDDQVNDMYDKESDRLQEEADKLKEELEKKYSDENLVDLINEAISSGKFVG
                     LDGEVEKLQDAIIEYINKYEDGMLAMGSVTKQEWLDTLKEGKETLEDINDILDELDLS
                     KFAMPNYNPPSNSRSRSAFPTSSVNFNSPFFVVQGNVTKDTMKDLEKFGRDLENRVYR
                     KIVENIKS"
     gene            complement(20509..21987)
                     /locus_tag="PC414_002449"
     CDS             complement(20509..21987)
                     /locus_tag="PC414_002449"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464837.1"
                     /translation="MVEEFKIKTSIELDDKDAREKIEKLKNSGQDKPIKLNLKIGELD
                     KLSQQAEQLKGIFNKAFKIDNTALSNLKQIESTMKEINKLSRRSQESLLGGGSGNTGN
                     INNEQQDLIRQYKEISNVVSRLQNQIRTGLGDSSIERTTEQIERLKRQLDSVYAQMNS
                     SSQARMDMFDASRATRDLADMNRNLSRIEDMATQASARLNGLGDNIDTDKVNRLRNEL
                     QELKQTASRNIDLNLDVGNIVNRIQEINQEVRNLERVDSLRSNFSRIEDSIRQAFGNE
                     RVEAFRRSLEQLQGSAGNLNGDFNNIFDRLTREMRDARQEAERLNNTVSRGGGFFNDF
                     YSSVASYSIGDMLGDALTDGIRSMKDVILELDSSMTDLKKVAEPTDIDSTSELDRIRE
                     KAIQTAKDVGMSASQVQESIASSLQAGIGGMQESIAAARQSMILANVGDMDVEGASSA
                     VNTIINGFRIEPLKEMQVQVGNTTKKTTELTAAMDMLNCLAC"
     gene            complement(22067..22516)
                     /locus_tag="PC414_002450"
     CDS             complement(22067..22516)
                     /locus_tag="PC414_002450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464838.1"
                     /translation="MFKKILLILMISLLGFGVIGCSDSKEDTNEVVKKETEEKLDEES
                     TSEDIYNKKLQFLNDTKNQSEIIGDEIRLLTEKYNNYEIVKSDWKEDLISKKKIITDL
                     KSVLEEEKSNILDGVSDFQKTILQNKYNEIIEYYDYMLKGISQNLNI"
     gene            complement(22605..23579)
                     /locus_tag="PC414_002451"
     CDS             complement(22605..23579)
                     /locus_tag="PC414_002451"
                     /inference="COORDINATES: protein motif:HMM:NF024352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464839.1"
                     /translation="MDNKIVIQSNPIILKMLKCKHKLGDYLNLHKGWMSVPNKTICDG
                     IIYDKKILSSNDIKDNNQLNGILNKCLEGKDIHRYYIDKVDKFVNIENMDDKTMRWHK
                     SPKIITQRIVGQNQNKIIATVDIEGTIIFPSGNIINLIKEDNLEDIYFYIGVINSSLI
                     NYFYNKFYGESNTNLTADAVKNIPIPNLKNVDKSIVINNSKILINKYGKLYLYTNKFL
                     KNVFKILSIDNNPSNKLYNFYLLDFDEFYNELSKKKKLYFKNIDELEEYFNKYKDAIN
                     RIIYDINVCENTINTFVYDLYGITELESKSIEDICKTTKDTVVTSLAY"
BASE COUNT         7120 a         4082 c         2544 g         9910 t
ORIGIN      
        1 taaccaaaat tccattcagt ctgcattttc tcactctcct ctctaacaat aagttattaa
       61 ctcatttaca ctagttgcaa tattagataa accaccattt accttttctt ctagattaac
      121 aaatctatct tcgattttct tagacgaata agtagtcatt tcagatactc tgttatcatc
      181 tacagttgca ttaataaaat gagtttctgc atttccattt atcacataaa cgtttaattc
      241 tgaccttgtt tcacttctaa tttctataga attatcatct ataattttaa aatttgtaac
      301 tacattttct tttgtagtag catctataat atttacaact attctctgtg ttaacaaacc
      361 atgtgttaca gttgctttaa atccattttc tgtatcatcc acccaatcat caattgttat
      421 tgtttgagta gatgccacat ttgaaccacc tgcgataagt tggtcaattt tagtattcaa
      481 ctctgtttta gcagtttcta tatttgtatt aactttttct atttctgtat tagtgttatt
      541 aggtacattt tttaattttt ttactacatc ttcatttaac aatttatcac cttctttttt
      601 atttagagag ctacattagt aactctctat tttgaacata aaaaaaagaa ctcttacagt
      661 tctatgataa tgaaatctct agtgcttcac acatttttct tacttcttct cgatatttaa
      721 caggtacttg gtcaattgtt tttaaacctc tctctatcaa tcctacataa attgctgcca
      781 tactacttcc tccttcattt aattttatat tttttaatga catagtagat gtctgttgac
      841 tacttaatac catttcgcat atttcagcag tcgctaacat taaatctgtt atgtcattat
      901 cttgcgtttt ttgacttgaa tctaaatcat ctaattcttt tctttgtctt gacttctcaa
      961 ttttttcttt atctattttt atcatttaat cacctcttaa agtgtttcaa catctaaatt
     1021 gaataacata gatggttttt ttatagttgt attagtgttt ccttcttcta ctattacttt
     1081 aggttgtgca gattgtatat tcatatcttt taactcatat atctccttat atccattctc
     1141 agtttttctt gtctctactt taaccatatc tttaaatgta tctgattcat ttatctttaa
     1201 ttgtactttt aatttattta ctaaatgata aattggattg ttagaattta aatcttctaa
     1261 cagaccttta ttgagtgtaa gtactacatc ttgtgaatta tcaactatac tagatacatt
     1321 ataagtattc ccatcgatta atacagttga atcttttact atagttgctc ctgtttcttt
     1381 tttataaata taaacattta tatataaatt atttccaatg tcagaatgtg ctttaaatac
     1441 aatcttattt tctccaagaa ctaaattctc tggattaatc tcaaacgtga ctgttcctgt
     1501 agataagtct tctattatac ttgaactaat attatttata aacatctcta tttttgtcaa
     1561 ttcattattt ggattttgca aatctatttc aatagatgag tatgacttat tctttatttc
     1621 caaacaagtt ttctctgaat ctacttttga gattatatat aatgtttcat cttttattaa
     1681 agcatccact atatcattaa catctcttgg aatatatatt tgtgagaaag tatataacat
     1741 agaaccaatc tctacatctt taattttata tgtttcctta tttatccctg taacatacaa
     1801 agtcttatca ttttttataa caaatatatg tttatcatca taagatttta tatattttac
     1861 attttctaat actttagtaa ataatttaat aattgtattt tttgtattct cattcagtcc
     1921 taaaatccca tttatattat atccttgtac gtataattga ccttctgtag ttatagccaa
     1981 tatataatta ctataagcat atatctccct tatattagaa atagttaata cttcttgtaa
     2041 ctcattaacc tcatcattat gccctattcc aagttgatat tctgagtttt taccaataga
     2101 aaacaatgag ccatcatttt ttaagaagta agtaaaatta tcaccacaag caatatctat
     2161 tatatttgtt aagttaattt ttataaattc agaagttgtt gttagctcag atttatttat
     2221 agcaagttcc ccaaattgat ttgaaccaca accatagaca tccccatttt ctaatatcaa
     2281 aattgaatga gttgatgata aagacatctt ttttatatta tcaaaaggca atgaagttga
     2341 gtaatttcca gcatttgtgc caaatggtat tctataatta atactatcat cattatgtaa
     2401 aaatatcttc ttatttacta atagtccata attatttctt ataatagaca tttcattaga
     2461 aaaacttaaa tcactaggat tatcaccaga agtgtttgca atctgatttt cttcatgtcc
     2521 attaaaaata cctacttgac caactacagc attacctttt ccttctaatt tattattagt
     2581 atattgaact ataagaccat tatgctgtgt atatacctgt tttacattaa ggcaatttct
     2641 aacaggtgtt ttttgtacaa catcagaata attattgatt tgacccaatt gtctactacc
     2701 aaacacatat aaggcattat cattatctat ataataagca tttttaccat ttgcatatat
     2761 gtgctttata ttacttgtta atttttttaa tactacagta ccagattcat taaaacctat
     2821 accatcatca gcatttcctc cagaatataa atctccattt tcaagtaaaa caaaaatagt
     2881 gtattctcct agagcaacct cttttatatt actattaaac gctaaatcct tatatccttc
     2941 aatatatcct gatgaattat tatatgaagt accaacacct aaacctccta atgagttata
     3001 tcctgtgaca aatattctat ttttcattgt aattatagtt ttgccttctt ccaacattaa
     3061 atttgatata gttgatgggt ctgttaatcc ttcaatattt attttagtta aactgtttga
     3121 atttgtattt gaacttatgt aatttctatt tccacaaccc attaaagaaa ttatattatc
     3181 attctccaat aatataataa aaacatacct tccacaaaat atatccttta ttgttccaga
     3241 aatatttact ttagtaaaag tatttctatt aatattatcc cctaatccta attgaccata
     3301 gccatttgaa ccagttgaaa ataattcatt gtcataggtt agaatgtaag aagatttgta
     3361 acatgttatt acttttttta cattatcaat aggcacctta gtaaaagtac ccctatcaat
     3421 attatctcct aaacctaatt gccctaagtc atttgttcca attgaataga cttgattatt
     3481 atttgtaaga ataaataaac aatcattcga tggaataaat tcttttatat tatctatagg
     3541 aacttttata aattcagacc gagctttaat tgtccctaac ttgttaccag cattattacc
     3601 acagacatat ccttcgctat tatttaataa tatatataaa gcttgtctat cacttttaat
     3661 ttcttttata tttgatatga tatatttttt agaaaattgt tttgatatat ttacaaaacc
     3721 atcatgtcta acatcataag gtttttttat gccaaaacag ccatgagaat ttactccaga
     3781 acaatatatg ttcccaaaat aatctttaat tactatcctt aatctactag catatattac
     3841 ttgtgaatta tatgcaaaaa aagcatttga taatgtgtat ttttgactat tagaataata
     3901 actactgtct cctctttgcc cacctgcatt tctacctctt atttcaaatt tagactccat
     3961 ccttgtttta cttgttgtat ataaaagatt gtctgttcca agataatgat tataataagg
     4021 catagaagac gatataagac ttcttaaacc taaattcttg tcttgggatg aagctccaaa
     4081 agtcgaaggt cttcctgtga attttatttc tttatatcct ccatcactag catctaataa
     4141 taaacataaa ttatagttag aaaccagaac ttttgataat ttaggttcat ctattaaaac
     4201 tgcgacttca tttgcatttg agtttttact tcctacaaac tctccaaacc ttgaataata
     4261 atcatcagtt acagaatttt taatacttgt gctataataa ttatgtctcc cagaaacata
     4321 gtagttatta gacgtagttc taattaaaca tccattatta aaaggaataa tttcagcaat
     4381 gttagataca tctgatactg ttgtatctgt tttatatctt aaatatttca tcttaacaaa
     4441 aacgctccta cttgaggtat cattaagact taattcgcca aaaacattat atcctgctgc
     4501 ataagatatt ctgctattgt ctatgcaata tgaagaattt gaatatgcaa taatttcatt
     4561 tacataagtt acagtactta ctttagtaaa aatatttcta ttagtattgt cattaagtcc
     4621 taactgccca taattgttta aaccagttga aaataattca ttagtagttg tttttaataa
     4681 cgcatggtta tctcctacag aaattttgtc aacagaagaa atatttactt tagtaaaagt
     4741 atttctatta atattatccc ctaatcctaa cactccaaat acattatatc ctgttgaata
     4801 taatgtatta tcacttagta atataaacgt gaaatatgag cctagattta aatatatatc
     4861 ttttatagta actccttctt ctaattccaa tggcactcta gtaaactcat aacaatgacg
     4921 attatgacct aaacctaaac ctccataagc gttgtatcct gctgcgtata gttcattacc
     4981 attcacaaca ataaatgtag aggtgtcatt tgctttcact aaagagatat tcacaaaatt
     5041 attttcaagc cctatatcta aaactctttc atcattttta tttattaata atctattatc
     5101 aatagactca atatgattac attcttcaat atcaaagtcc tcaaattttt caaatccata
     5161 tataaattta tcatatcttt ttaaataaga aaaactataa ttgttatctc ctgtcatata
     5221 caattcattg ttgctagtta atataaagct tatagcatga gcacatttaa ttttacttat
     5281 atttttaata ctaatagaag attttctagg agtatatgtt gatctatcac aatttatttt
     5341 atctcctaaa cgcccatctg ggttggctcc agttacatat atatcattat tattagctat
     5401 ataaaattta tgatatctac cactttctat attttttaca ttagatatag atagttttgt
     5461 taagactgtt actgtagctc ctccaccaac accaacttgc ccacttgaat tatctccaca
     5521 accatagaca tccccatttt ctaaaagaaa atttacagca ccttgagcag tagaaatctt
     5581 tattacatta tctaaattta ctttagtaaa aatatttcta ttagtagtgt cattaagtcc
     5641 taactgccca taattgttta aaccagttga aaataattca ttatttaaag ttaatatata
     5701 tgaacaactg actcctgcaa atatttcttt aacactcaaa ttatcaattt tagtaaatgt
     5761 atttacagta gatgtatttc ctaagcctat ccctccaaat ttattattac cacatacata
     5821 aagctcgttg tcatttgtaa ctaacataga atagccattc ccacaagcaa tttgttttac
     5881 attacttata tttatcttag taaaagtatt tctatcaata ttatctccca aacctaactc
     5941 cccatctgtg tttaagccag tagaatacac atctccgtta tttttaagta aaagagaatg
     6001 agttccagaa gatgaaacat acttaacatt gtcaatatct atttttgaaa aatatcttat
     6061 aggtgatact ttatttccaa gtcctaaatt gccaaaaaca ttatatcctg ctgcatataa
     6121 ctttaaatca tccttaatta taaacacaga accagaatcc atatctgtaa atatatcttt
     6181 aacattaaaa gtatcaaaat ttttattaaa ctcttctatt ttaaattctt tgtttccaaa
     6241 taaagaacct ctttgtaata gtttattctt tttcatataa ttccccctat ctcacttcgt
     6301 aggaatactt gtttattttt atttcagtat cacctttttg ataagattct tctgttttag
     6361 tttcactata gttgcttcct aaatcatgaa tatatcttat tttgttgcat aaaaaaggaa
     6421 ctgttacatt tttaaataat acagttccac tatttgaaag taactctaat tgttgcttaa
     6481 atttattaag ctttaaacca tctaaattac ttatgttttt agaatcatat agggtatcat
     6541 accaataccc agcttcatct gctgtagctt ccttatctaa ttctaattca aatagaattt
     6601 taagtatttc cacagacatt ttattataat tttcttttaa tattttcaat tgctcgtttg
     6661 tatttccacc agattcaaga actttaacac tagcatctaa ttcatttacc gcatctgata
     6721 tatttttaga tactgtactt aattcactcg aaccaacttt actttgtatt agatttattt
     6781 tctctaagtt agtagctata ttacttttat tttcatttat cttagttaga ctctcatcaa
     6841 attttgcatc aattttaata ctagagtaag tactatctag tgttgttata ttatcattta
     6901 ttgtagcttg tataacttct ttatttgtat taccatttac aacagttact tttacatcta
     6961 tgttactttc attaaatatt tctatcgtat tagaatctac tgtagtataa gaattagtca
     7021 tacttttctt agttgcttca tctaatattg ctacaaatat attttctgta actaaattat
     7081 gattaactat aagtttaaaa tattctccat cagcaaccca atccgattca acataagatt
     7141 tactaaaagc tacattagtt gcagcagaag tcatttctgc tattttctcg ttaactttct
     7201 caaagtcata tgttaatcta tcctttaaag tatcctttac agttccatca acactttctc
     7261 tagcttgtat taattcgcct ctagcatcaa gactttctaa ttcttcaaac ctattttcaa
     7321 attcagttat tttattacta acagttgttg tcatatcagt tttagcagta tttacttcaa
     7381 ctattttgtc atctacttct tttatctttg tatctaattt tgtagtactt tcatcaatac
     7441 aatcttctac attagatata gcttgattta cttcatcaag tttgtaatca atttgctctt
     7501 ggttatctgt aagtttatct tcaaatgtct tatcatacaa atcaaaagta ttattagctc
     7561 tagtgtctaa gtcttgaaac ttcttgtctg tctgaacttg aacatcaagc attttatcat
     7621 cgactatatt atctatttct ttagacttag catctatcat ttcatctaat tggagttggc
     7681 aatctgtaag tttatcatca acttgaattt gcatattctg aatggattct tcacgatttg
     7741 cctcttgatt ttgacgtgtt aactcattaa actctctttc ttcttcttgt atgtttctta
     7801 tatcttcatt ataaatccta atctgctctg ctttatccct aacttcttca tttccaagta
     7861 tagttatttc aagctgttca actcttttag tcaactcttt attatttttt ataatctctt
     7921 ctgcttgggc tattattacc aataatactc catattcatc agttgaggtg atatcatcat
     7981 cattaatcat accctcttca actctataat aaagatttgt agtattgagt aaagttccat
     8041 ctttcacaag ggatagttga aatgtattta taccaacaga agcaagtgct tgtcggctaa
     8101 gttctatctc tacaatacca ttttctgcat ctacaacttc acattcacca ttaacctttt
     8161 gtccatcact ttttgtaatg ctaactaaaa ctctatcata ttcagataaa tctattattt
     8221 catctttatt tttcaatata gcttcaatat aagctgtttt aacatcattt tcattataaa
     8281 taaaataatc tagcaatctt ttgtttattc tataattact aaagtcgacc tctatggtat
     8341 gattctttat ttcataattt ttcaaagtat cacatcctat tgttctttat tttgttcttc
     8401 tagttctttt ttcaagttct caatttcttc ttgttgttgc atgttgagtg ctttataaag
     8461 tattagttca ttatataaac tttttatctc ttcaaatgct actgtatatg ctttttgtaa
     8521 atttacttcc atattaatat cctcctaaat tattaatttt ctcgtttaat tcattaatta
     8581 tcatctctaa ttcttctctt ttttgtattt cttcttgaag tgcagttgct acaacagttg
     8641 tataaccaga tgggctaaac attaatccat tttcttctcc atagtcatat agaaatgttt
     8701 ttccaacata actatcttta aaatcattag ctataaaccc tatttgacta tctgcaattg
     8761 tcctatcttt ttctgctata taatcaaatg ttgctggttt aaaatcatct cttataaaat
     8821 ctaaaaatgg tgttggagtg atacttgaac gagttctatt cttaatatct tttatgtata
     8881 ctatattctc tttaaacttt ttatcagatt cactcagagt agaacgagca tataaatatc
     8941 tccatttata acgctcacaa cctaaatcag ttacagaacg ctctggataa aatgaatatc
     9001 ctacacctaa actatctcca tttgtaaatg taaaagtagc tccaccacat cttacaaaag
     9061 tatcatctct ttcaacaata aagtgttctt ctccatcaac ataaatctca gcagtatcat
     9121 atcctaccca aaaataatta ttgctatcat attttaatct tattgctgag gcttgactag
     9181 aaccatctga ttgtcttgca tctattgcaa atccagacct gctacttcca aataatctga
     9241 ttattggttc atgtccagat ggagtagtta atatatcaca tgctatagct ccttgattta
     9301 ctgttagacc actcctgctt atctctacat aatttgatat atcattaaat ccaaattcaa
     9361 ttttatcagg tttaattcct acaattgagc cagagttatt cctcgtaacc atcatggtta
     9421 tatcatcttt agttaattta atttcagcag catttgtttt agttgcacca tcaacttttg
     9481 ctacttctaa ttttatttct ttagctgtag catttatctg tgaaaattcc tctgatatac
     9541 ttccacccat acctgttatg taaattttat cgactgtatt tttaagttca tttatcttta
     9601 agttatattg agcatttgca tttatagcta aaattctgtc tgaaggtgta attatactat
     9661 ctgaaataac tgaattaatt atagagttta gattggtact tgctgcatcc aatgctgttt
     9721 ttgaagtgtt tattgctact aagtctactc cagtagttct atcaataagt ttttgtagct
     9781 ctgtatataa acttgctttg tctgtatcta ataaattaag ttcaatatca atcttttctt
     9841 tttcttcatc tgtaacttca ttatcattaa aataattaga catattagaa ctaaatgtac
     9901 ctaaatctgc atttaactta attatagctt tatttatgtt ttctcttaat gttatatttt
     9961 cttgtgaacc accaaaattt ttagtaactt cttttactaa tatatttaca ttttcagctc
    10021 tttggtctat aagagacata ttttctttta tatgagatgc atcttcttgt acttctaaaa
    10081 ctgtttgatg tattccatct atatcttttt caataactac aaacttttca ttatgctttt
    10141 catcagtctc atataatccc caaatctgtt cttctgtatt ttttcttgaa ttaccatcaa
    10201 atgaaatatc aaaaatagaa gaatttatag gaacttctgt gtgttttcca ttgtcattta
    10261 caactactaa aaattgtgtg tttccctctg gtaatcttac attagaaact tttaatgttc
    10321 ctggattatc agcatcccag tatatatatt ttttatttgt ctctgtatct actacttgat
    10381 aaagtgtatt gttgtaggta acaattactc cagtaattct aatccatgaa tctgctggtg
    10441 aattatctaa tatcaattta catcagctcc ttttgtatat ttttcttagg aatatattca
    10501 tctggtagat taattctttt cttctgttgc tgaataaata aatgtctctt agagtttaat
    10561 gttctcgttg cgtgttcagc tttagtcaaa tagtcagcta tagtcctttt gtcatcttct
    10621 tttacttttt tattacttaa agttaattct aaagtatcca atttcccatt tttaaaattt
    10681 tgagtaaaag atgtaaaata gactaattct tcttctttag attctttgct atgtaacact
    10741 ataatatctc ccagacttaa atcacccttc caatgttgtc taaaattaat atctattatt
    10801 ctatctaaga agtttataac atccagagtc cactctttgg ttggaataca ttttaaactt
    10861 aattgtctct taccttctgc tattaaatct tcaactttta aaaaagcatc atttgtataa
    10921 gtgtcataat agagaaactc atttaactca tccaatagag cctcattaaa tattaaatat
    10981 ccatcctcat cagtcgaggt ttctctctta caaagaatat ttatatcttt tatactttca
    11041 tctaactttg ctatctcttc ttctaaatgt tttatttgaa catctaatat gaccttttta
    11101 tcttctagtt cacttatttc tactgctaac ttagctgaat ttacttcatc tttatgttct
    11161 ggattatcat aagttttctt tatatccttt ttagccttta tagtctctat tacatgtaac
    11221 cattcattac ttttactatc acgttctctc tgtttcttaa gttttgtatc tattagttct
    11281 ctccaaattg gttctcttat ttcattcatt tcctggtatt tctttatagc tctactaagt
    11341 tcttcactca tttctttatt gtctaagaaa taagaataat tctcaatgta atcatatcct
    11401 gtaactgtag ctcctattat atccatatct tcactacctt caagcttcag tctagttact
    11461 atatcatcac tatttgtagt tctttcaagt gattttatgt agttatcatg agataaatat
    11521 aactgaatgt tatctccaaa actgtctata tgatacaaat taactaaatt attataagtg
    11581 tcaaaatcag ctatacactc aaattgttct tttagttcat tattgaaata atctaaccaa
    11641 ttagaattaa tactctcttg ccatcttact ttttctctct tattaccttc actatcagtt
    11701 tcataagcaa ttgaatcatc cacataacca agtttccagc ctgtctcttg ttttaaataa
    11761 tcattaagag atataataga tgcttcttca tctttggtaa acatctgtaa tccatagtcc
    11821 tcaatattca catcaatttt tcctagttta acttctttag atttagctgt tactactttt
    11881 aatttatcgt ctgtagtcac aacattctta actacaaaat attctttatt attaagacaa
    11941 ataagacgtt cttcttttac ttcttcaaaa atgggattta ttattttatt aaataaaaat
    12001 ctatctgtaa tatattttgg aatggtcagt tgtatttcat ctactccacc aagctcacgc
    12061 tttatagaat ttaaaaagtt gattgggatt tgacctaaaa atgatttgtt catcttgtga
    12121 atggttaatg tgtaatcaga ttttatctgt ttaagattca cattaaaatg catattttat
    12181 caccttcttt gtattagaaa agcccatcaa ttaagatgag ctaaaatatt ttattaatct
    12241 aaatattgta acaagtttaa tagcttgtta catatatgat aaagcaaaaa accactcaaa
    12301 cgccaataag agtagtttca aaagataaat tgatttttaa ttaactttaa ggaaacaatc
    12361 actctttgta ttaagtagat taaattcctt acttttatta taccacagat acgttttttg
    12421 taaaacagat tcgtttacag ttaataaata aaaaatcact agataaagac gattactgat
    12481 atcaactaat acatatttaa aataggtata ttataaatca tttcattatt caaaattcac
    12541 ttcaatatct tcaaggtctt tttcattaat atttaggtct ttcagaatat ccctttgaga
    12601 tattagttct ttttcatcta tatctttcat tcttatttct gcctcaataa gaagcttaca
    12661 atcttctata ttatttatca tttgtaacac ccttaaatat ataccagaat agaaaaatag
    12721 ccacttttta aggtggcata gataagtagt aattcttatg taaaattttt aaattttctg
    12781 ttatctgtat tatatcttta ttctactcct gtcgtcaata taatattaaa tttaacatat
    12841 tggaaaatta tggtataata tttttagaga ctacagttta gactattagg gtgatggctc
    12901 tttagttaaa atccccagta agaaaggggg tgagactata gagtacttag ttttgttttt
    12961 aataatgata attatgatta caagaataat caatcatttg acaaagctaa tagatgcaat
    13021 cttaaacttg aaagcatcta ttaggaaact aaagaatcac cagactggcg ggtctagtga
    13081 ttctgaaagt aatacataaa tctattcttt ttattgttaa ctttaagaac tacaccctac
    13141 agcaaatagt ttgtagtctc ttttttatgt ccctatgata taaatttata tcatttatat
    13201 tactattata ctccaatatt atcaaaatag tcaatgttgt cattatcttc attgaaaaat
    13261 aaaaaagcta tattattaat agaataactt cttagaacac aatattcttt tacaattgta
    13321 gacaatataa tattaaattt aacatattgg taatttatgg tataatattt ttagagacta
    13381 cataaaaaaa actattaggg tgaatcttca tatacataag ccttcccatg tcaaatcaag
    13441 aggggaggtg atatgatatt atgaaatatc taatttgttt gttaacgata ataacatttc
    13501 ttacaactat aattattagt ttgattaagt tggttcagaa tttaaataga ctctttaaag
    13561 aaatagatga gtttaagaaa acgttgcaaa aaattaagaa tcacccgaac ggcaattcag
    13621 gtgattcttc ctctaattaa cacttcgctt atttatgaag agacacccta gcacaaatag
    13681 ttgtagtctc tttttttatg tctttatgat ataaatttat atcatttata ttactattat
    13741 accatataca tattattttt tgaaataaaa tctggatttt attagagaaa tctggatttc
    13801 tacttaaaat cctttgtcta tataaataat ctatgattta atattttcta ctatttttct
    13861 atatactcta ttctctaaat ctctaccaaa tttttccaag tctttcatag tatccttagt
    13921 tacatttcct tgaacaacaa agaatggtga attaaaatta actgaagatg ttggaaatgc
    13981 acttcttgaa cgagaatttg aaggaggatt ataattaggc atagcaaact tacttaaatc
    14041 taattcatct aatatgtcat taatatcttc taatgtctct ttaccttctt ttagtgtatc
    14101 aagccattct tgcttagtaa cagaacccat tgccaacatg ccatcttcat atttgtttat
    14161 gtattcaatg atagcatctt gaagtttctc aacttctcca tcaagaccta caaatttacc
    14221 agaactgatt gcttcattaa ttaaatcaac taaattttca tctgagtatt ttttctctaa
    14281 ctcttctttt aatttatctg cttcttcttg tagtctgtca gactccttat catacatatc
    14341 attgacttgg tcgtctacat ggtcttgtac taaatcctgt aatttcttct gttcttcttt
    14401 taattgtttt tgtaaatctt taagtttctt ctgaccactt aaagaactat ctctttcagc
    14461 tattgctatt tgtttttcta aatcacttat tacatcttgt tgttcttcat aatcatcttt
    14521 atatttggct tcttttcttg aatcattgta agcatcttgt tgctttttaa gagaatcaat
    14581 tttagcttta gtttccttgt ctatttcttt aagtctctct tcaagttctt ttttatacat
    14641 gtctctaatt ttctcttgta gactttgagc ttctttgagt tggtctttat aagcatcttg
    14701 aatttcttta tttaatgctt caatttcttt cttagattca gcaatttcct tattagcatc
    14761 aggtatttta tttattaata aatccaaata tccctctgct gcatcttcca ttcttttaaa
    14821 ttcttcatca gtcatgttct ttttaagttg atttttatgt tgaatgaagt tagttatatc
    14881 gccattatct ctaaatgtaa atccatagcc ttgtagcttt tgttgtagct ctttttgtgt
    14941 ttcttgaaga tatgctgttg tttcagatag ttctttagcc ttcttggcca ttaactcttt
    15001 tttcttttct aacaactcta tctgctcatc acctgtagca tttttaagta atacatctat
    15061 cattgctatt tcattattta cttgctctac atctttgtac atagaagtcc atgtggaatt
    15121 cttattaagt ttccacattt cttcttgtgc tttccttaat tcctcattgt gttctttaat
    15181 tttattattg tattcttccc attcttgctc acatttaggc agttctgtaa atgtaacttt
    15241 aatatattct tcaaggactt tcttagtttc ttccaactct ttttttctgg attcatcttt
    15301 tttattttct ttgtcaccag actttgaatt taatttgtct aattcttttt ctattgatat
    15361 taacttttct tcatagttag ttagatttcc agtatcatca aacttaaagc ccattttctt
    15421 ttcttttaat ctatttttaa gtctgttact ttcagcttta tatccatctt gttgttgttt
    15481 tattaaatct ttttgttgag caaataattt attttgctcc tgtaagtatt taatcttctc
    15541 atcacctgta gcatgttcca tttttttgtc taatagagct atttcatttg ttactgctga
    15601 tatagcattt tctaactctt taaataattc tatgctatat tttacagatg agtctattgt
    15661 gtcattgttt attgtaactt taggtttgat tactttaaaa gctgatgtgg ctcttgatac
    15721 tgctcttgag aataaggatg gttttgttac aggagtttcg tcacttattg gtgctggttc
    15781 tgatgttcta ggttgggata gattagctgg aattggagtt ccattagtaa cagaggtacg
    15841 ttcagtttta ttggtcttat ctgaactatt tcctccacct ccaccaccag aactattggt
    15901 tactttatta gttacaaatg tagtagtgaa tgtttttcct gcatagtttg atttaaatga
    15961 ctctattttc ctaataagcc ctgatatgtt ctttgcagca gttgcagtta atgcactaaa
    16021 tgtagtcttt atagtttttc caccatatcg gctaacataa ctttgtactt tggctaacaa
    16081 acctgataca ttttttgctg cttgtgcagt ttcagttcta aatacaagtg tagatacttt
    16141 caaagctaag aattgtttta ctctagctat aagtcctgaa atattcttag ccccttgttc
    16201 agttgaagta ctaacattta tatgataatt accttgccca atttcttgtg ctttttgtga
    16261 taattgattt aactgttcag atgcttgttc tgcacctgta gcttcaatag taggagtggc
    16321 tttttcatta ttaagttcat ctaacttttg ttttgtattg tcaatttcat ctttaccaga
    16381 gttgtttatg gataactcaa taaccttagt tggatttgta ttttctataa aattggttgc
    16441 ctgttcaact tcagtttctc cttctgtctt tatttgtaca gttggagtta ctgttttatt
    16501 atctaactca tccatttttt gtttagtact gttaagtcct tcttcattgg taacttgagg
    16561 acttacaatt ggaatataag attcatttcc atattcatct aatattctca tgcactgaac
    16621 aactttttca ctattattaa cttgaggatt taaattagga gtgtatgttt tattcccatt
    16681 cttatctaat gtttctatgc attcaactac ttgtgattct ccatttactt ctgcatttac
    16741 taaaaggtca gctttagttc catctgcatt ttgaactgta ttaacagttt tctctgcatt
    16801 ttctcctcct tcaacttcta ttccaagttg tttttttatt tctggaggta aattgtttat
    16861 aaattcttgt acatttttag ctttttcaag ttggtctaaa ttgtttacta taaaatcaat
    16921 aacagcttcc tttttatcat ttggaacatt attaagaatt tcatctatct catttagttt
    16981 atctggattt ttattgatta tttcaaatat agtttctttt ttatttggag ctttatctat
    17041 taagttttgt aatgtgtcta atttatctat acctattact tctgtttcta tctttttttt
    17101 gagattatca ggtaaaatat catagacttt ttttgcttca gtcaatcctt ttttatctac
    17161 atctatatca tattttagac tcattggagc agatattttt tttctagctt cctctaaaga
    17221 atttacatct ttaaaagcat ctgcacaatc tgtagtaaaa gacaactttg tatcttcatt
    17281 tttaaatcta tccaactgtt cttttaatac actcaagtct ttttttgcga aatctagtcc
    17341 tttattatta atttcagtca caattttatc tgatttaaaa gtgtcttttt tgaatttttc
    17401 taattctttt aattcttttt tagctttttc tccattgaca ctaggagtga tttcaggggc
    17461 attgtctcct gtaaaagcat ttgcaaattg ctcatttaat gcttgaacca tatttcctgg
    17521 taatgcaggt aacttgtcac tgatttttag tccattttta agaatgtcat ctgttaattg
    17581 taatttaccc ttaaataagt catttaattg tttaacaaca tcattagata attgaccttc
    17641 gttttgcaac gtagttgtta cttgtagggc taattgagcc tgagcttcta tttgtgaatc
    17701 ttctccaaat gtgaatccat ttattatatc ttttagttgt tgtggtaaat cttgaccagc
    17761 ttctccactt tgtatatctt caagcagttt tatatttact ttaccattaa cttctaaatc
    17821 ttgagaaaaa tcatttaatg catcaaattg gtttttaagc tttattgcaa aactatcttt
    17881 gttttcaagt tcagctaaag atgaaccata acttttcaag aaagtatgta atttcatact
    17941 ggctaagtct aatccagaaa aaccttgttg taatccaata agccaagtat cagcacttat
    18001 acttccacca cttagctttt gaagttcatt cgccaaccct tgtaattctt tggtatattg
    18061 attcatatca ccggatgcct gataatttat attagcatca gttatcttct tattccactg
    18121 gtctaatttt tcaacaggaa ttgactcact tattttatca aaagcactta taatgctatt
    18181 ctgttgtcct acactatagt catgccaatt catattagct acaagctcat tcattttact
    18241 tttcttttgt tcagattgtt tagcatatgt tttagatata tcagaattag ttaatctatt
    18301 aatcgccctt ttctgcatta cctcttcttc ttcattatat ttttcatatt cagtaagaag
    18361 tttttgatga tgttctgcaa aatctgtttc ttgttgattg taagcctttt ttaatgcttc
    18421 tgtttgttct ttaaaattag atttacttaa agcattttta aataaattat gctcatcttc
    18481 aagaacttta tcaagttttt tcttttcttt atcaaggtct ttttttgtat ctggtgcagt
    18541 ctttgatgta gttattgcaa gttcatcttc ttgagacttt aaaagtttat ttttttcttc
    18601 tatagccttt cttaatttat taatgacctc atctaagtta ccactcatat ttagaatagg
    18661 gtcattattc tcatctacac ccaatactaa ttctggtgct atatctgcaa tttgttgttt
    18721 aagttcgcta aatctttcag tttcatcagc agtcttttcg gtcttactag ctaaattatt
    18781 gtactcttct gatatacctt ctaaactctt tttcttttgt tgcaaggagc ttatctcgct
    18841 tgtagtagtt ccaataactt ctttttgttt ttctgctaat ttttcatact tgtgaatagt
    18901 atcatctatc cattgtatac ctttagttat agcaaaagta gccaaaccta tcatagcccc
    18961 gtttaacaaa cttgtcgcta tatttaatgt tccaatacca atagaagtcc tagataaact
    19021 ttgtcttaat gctcccaaac ctgttcctgc tctagtaatt acatttgctc cattagcacc
    19081 agctacattt gctgccatta ctgcatgtct aaatgattgt agtattgtag ttgtagcttt
    19141 aataggagtt cctatcatat ttaaaggatt tataagtcta gccacatttc tagtaccatt
    19201 tgtcattccc ataccagaga ataacgtttt taaagtcata aaacttccta ttaatgctgg
    19261 tgttgttaat tttaatcttt ctaatgtttt tataacacta tttattccat tagatacaga
    19321 tattaaacca tctaaaaatc catatgttgt atttgaatta aatacagttt ggaatatatc
    19381 tgcccatatt gttttcaact catttaaacg ccctgcaaga ctatctaaat attgtttgtt
    19441 ttcagcctca gccgaaccaa attgtaaccc ttgtgaaaat tcacttctta tttctttaaa
    19501 agtctcataa ttacccatta aggattggaa tatagctgct tgttgcttac ctgctattgc
    19561 ttcagctaat cctttttgtt catcctctct gagttctccc catttgcctt tcaactcatc
    19621 taagagttga accatattct tcaattcacc agtatcttta tttttataaa tagttattcc
    19681 agctatttcc tttaaacctt ttgcggtttt atttaaagaa agtgtaccat catcagctga
    19741 aactttcata cctgctaaat ttattgcgac actttttaaa ccattaccta ctttttctgg
    19801 attttgtatc gcttcatttg ttgctgttat catacccact gtgtcaccta aactgacacc
    19861 atactcatga agtacactac ctccacgttt ccgtttatct tcaattaagt tcgctacact
    19921 taatccgttc tcttatgaac tgctatatgt ttccatacag acgagactat atcttcatcc
    19981 tataaggatg ctccccattt ccatatccaa tagcttgata tgtacggttt ataacctagt
    20041 cgttgaacct tactctattg agtcttggct gctgattgtc cctatctttt agattattac
    20101 acttagtact aaaagcctaa caggagtttc cagcagttaa aggagtttat cattatatat
    20161 tactatataa ggcgactaaa attaatcgct tcggcaacac ctgctgttcc aattgcataa
    20221 ttattccctg catagtttca aattgttatc ctacaagctt tttatcttgt agttcttaca
    20281 tttcttattc atgtaagttc agcatatatt ttcatcttca agattacttg ttaagatgga
    20341 ggacactcgt gggaaaatta tattctatac tattttaaac ataaaaatag cataggttca
    20401 tttcctatgc gttacggtga ttaaagtctt ttaattcttt aatttacctc ggtggtaaca
    20461 tctcagtctt caccgatttt gccccctcat aatattagat atttctatct aacacgccaa
    20521 acaatttagc atatccattg cagcagttaa ttctgttgtt ttctttgttg tattcccgac
    20581 ttgtacttgc atttctttaa gtggttctat tctaaatcca ttgattattg tgtttactgc
    20641 cgaagatgca ccttcaacat ccatatcacc tacattggca agtatcattg attgtcttgc
    20701 agcagcaata ctttcttgca tgcctccaat tcctgcttgt aaagaagatg ctattgattc
    20761 ttgaacttgt gatgcactca ttcctacatc tttagctgtt tgtattgctt tttctcttat
    20821 tctatctaac tcagatgtac tatcaatatc tgttggttct gcaacctttt taaggtcagt
    20881 catagagcta tcaagctcta atataacatc tttcatagac ctaattccat cagtcaaagc
    20941 atcgcccaac atatctccaa tagaataaga tgctacagat gaatagaaat cattaaaaaa
    21001 tcctccacct cttgaaactg tattatttaa tctttcagct tcttgtctag catctctcat
    21061 ttctcttgtt aatctatcaa atatattgtt aaaatcacca tttagatttc ctgccgaacc
    21121 ttgaagttgc tctaaagaac gtctaaaagc ttcaactctc tcattcccaa atgcctgtct
    21181 aatagaatct tctattctgc tgaaattaga ccttaagcta tcaactcttt ctagatttct
    21241 aacttcttga tttatctctt gtattctatt aactatatta cctacatcaa gatttaaatc
    21301 tatatttcta ctagctgtct gttttaattc ttgtaactca ttcctaagtc tatttacttt
    21361 atctgtatct atattatcgc ctaatccatt aagcctagca gatgcttgtg ttgccatatc
    21421 ttctattctt gaaagatttc tattcatatc agctaaatct cttgttgctc ttgaagcatc
    21481 aaacatatcc attctagctt gagatgatga attcatttgt gcatacactg aatctaattg
    21541 tctttttaat ctttcaattt gttctgttgt tctttcaatt gaactatctc ctagacctgt
    21601 tctaatctga ttttgaagtc tagatacaac atttgagatt tctttatatt gtcttattaa
    21661 atcttgctgt tcattattta tatttcctgt gtttccagag ccaccaccaa gtaagctctc
    21721 ttgacttctt ctacttagtt tatttatttc tttcatagta gattcaattt gctttaagtt
    21781 gctcaaagca gtattatcaa tcttaaaggc tttattgaat atccctttta attgctctgc
    21841 ttgttgtgat aacttatcta attcacctat tttcaaattt aatttaatag gtttatcttg
    21901 accactattt tttaatttct ctattttttc tctagcatct ttgtcatcaa gctcaatact
    21961 ggttttaatt ttaaactctt caaccattta ctcaactcct tttttgtata aaaaaaagac
    22021 agttcatata gaaactgtac tttttcttta taaaaaatat ttaagtttaa atatttaagt
    22081 tctgacttat tcctttcaac atataatcat aatactctat tatttcatta tatttatttt
    22141 gtaatattgt tttttggaaa tcagatactc cgtctaaaat atttgacttt tcttcttcta
    22201 atacactttt taaatcagta attattttct ttttacttat taagtcttct ttccaatcac
    22261 ttttcacaat ttcatagtta ttatattttt cagtaagtaa tcttatttca tctcctataa
    22321 tttcactttg atttttagtg tcatttaaaa attgcaattt tttattataa atatcttcag
    22381 atgtactttc ttcatctaac ttttcttctg tttctttttt tactacttca tttgtatctt
    22441 ctttactatc actacatcca ataactccaa atcctaacaa actaatcatt aaaatcaata
    22501 atattttctt aaacataata tcccccctat attctgatac attaattata tcaattctaa
    22561 aggaaaattt atactacaaa aggaaagtga cctccaaatc tttattaata agctaaactt
    22621 gtaaccacag tgtcttttgt tgtcttacat atatcttcta tagatttact ttctagctca
    22681 gttataccgt acaaatcata tacaaaagta ttgattgtat tttcgcatac atttatatcg
    22741 tatatgattc tattaatagc atctttgtac ttattaaaat attcctctaa ctcatcaata
    22801 ttcttaaaat aaagcttctt ttttttgcta agttcattgt aaaattcatc aaaatctaat
    22861 aaataaaaat tatataattt attagatgga ttattatcta tgctcaatat cttaaataca
    22921 tttttcaaaa atttattagt gtataagtat aatttaccat atttatttat tagtatttta
    22981 ctattattta taacaattga tttatcaaca ttttttaaat ttggtattgg tatatttttt
    23041 actgcatctg ctgtcaagtt ggtatttgat tctccataaa atttattata aaagtaattt
    23101 attaaactag aatttataac tcctatataa aaatatatat cttccaaatt atcttcttta
    23161 attaaattta tgatatttcc actagggaat attatagtac cttcaatatc tactgttgca
    23221 attatcttat tttgattttg accaactatt ctttgtgtta ttatcttagg tgatttgtgc
    23281 cacctcatag ttttgtcatc catgttttca atatttacaa atttatctac tttatcaata
    23341 tagtatctat gtatatcctt accttccaaa catttattta aaattccatt taattggtta
    23401 ttatctttta tatcattact acttaatatt ttcttatcat atataattcc atcacaaatt
    23461 gttttattag ggacactcat ccaaccttta tgtaaattta aataatcccc taatttatgc
    23521 ttacatttga gcatttttaa tattattggg ttgctttgta taacaatttt attatccaaa
    23581 tcatttataa tactttttat agtaatttta ttttcagagt tgtatgtatt actattgaaa
    23641 atcttaatct ttttca
//
LOCUS       DAMJVK010000062        23212 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.063, whole genome shotgun sequence.
ACCESSION   DAMJVK010000062 DAMJVK010000000
VERSION     DAMJVK010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23212
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.063"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(162..530)
                     /locus_tag="PC414_002452"
     CDS             complement(162..530)
                     /locus_tag="PC414_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2464840.1"
                     /translation="MKKIPKSELLVMNFIWDKDIDKIPAREVANYMEISHGWTKGTTG
                     KVLSRLSDKGFLEFEKDGRNTLYTIKVKHEEYLKFETKDFLELIHKNSIHSFISSLGC
                     GNEISDDDIKELEQWLKKRK"
     gene            complement(1058..1480)
                     /locus_tag="PC414_002453"
     CDS             complement(1058..1480)
                     /locus_tag="PC414_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HDF2464841.1"
                     /translation="MNTFKTEVLLKYSAKDIFNIFTKSARLNFPNFNEKKAVGTFVQQ
                     KNNKRFKVEITNFEKNRVYEIKTSNKRESTITRYELVHIDLENTKLIFIESESKRNIF
                     GKINSALVRVLFGKRQPEEFNKFIKKLEMELENNSVNL"
     gene            1958..4861
                     /locus_tag="PC414_002454"
     CDS             1958..4861
                     /locus_tag="PC414_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2464842.1"
                     /translation="MELRDKLVNIINTENKKDPLTDVQIAKFLSTTRENITNLRKELN
                     ISNSRQRRYPYLKSAISAILQKNKNISISEITRELMTEGFNISRRVVEELLPKESLEM
                     DVVEEDEEESNDPFETLIGNKGSLRNAVEQAKSAILYPPKGLPTLIIGESGVGKSLFS
                     RHMYEFAKQKKIVKESSNFVVFNCADYSDNPQLLLSLLFGYKKGAFTGAEYDTPGLVE
                     EADEGVLFLDEIHRLPPKGQEILFSILDRGKFRRLGETNAERKVSIMLIGATTENVET
                     NLLLTFRRRIPMLITLPPLHDRLLKEKIDLIYNIFQQECNRINAKIFVDKNVIEILAL
                     KKFSGNIGQLQNMIQVLCARAFMKFINSKDEDSESIVVVDINEVLKLKDSFKDAAFQE
                     IEYTEIRKYLKNAIFIPFNLEESSVKGSLLSDEYILPEDIYKTIEKKYYDLKSLDIED
                     IDIENILWTFILNRFADLKFNLDSNEEIFSMSDLNSFVSDSLVKLVKELMNDIVEKNM
                     NLEVNKNMFKYLAIHLEEAIKRIKLNQKIINVNLEKIKIDFSKEYEISKSFAIKIEES
                     IGIKISDDEIGFITLYIKSALKNEVKKDKVGLIIISHGRIATETVNVVKELLGVKFPV
                     AIDMPLDEKPINIYNKAVELSKIIDQGKGILFLVDIGSLTNIGQIVNKRTNISTKTID
                     RVDLLMALEATRKVSMGEEELDEIFFSLLKDRMGYNYELEKHIDKSNAIITLCLTGEG
                     TAKYISKTLEEKYDNTKCYQMSALDESLFCKIENLKETNNILAIVGTINPKIPGINFI
                     PYNKELFKNLDIYLSKSKQQDDRPIKSYERMLDEDLVIFEPDIYFKKDLLEYVCSILI
                     NKEYVEKDYLDSVLHREEMLPTYSKGAIGVPHGDSSTVNSTRFVFVKLKNPIDWGVGN
                     VNFIFMPVFQANDKEIVKNVLEILKDAEFMNNANKCFDKDAFRRIIFDKFKHL"
     gene            4894..5340
                     /locus_tag="PC414_002455"
     CDS             4894..5340
                     /locus_tag="PC414_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2464843.1"
                     /translation="MNVDLISLGLESNDYESVIEELGSIMCKKEYVKETYINAVLERE
                     RTLPTGLDIGDMCVAIPHTDSKHVNESNVAVGILKNPVKFNSMIDPKDRLDVELVFLL
                     AVKNPDSQVKLLKDLMSVFQNIKLLKNIKNASTKEEVAKLLDFIEI"
     gene            5463..5765
                     /locus_tag="PC414_002456"
     CDS             5463..5765
                     /locus_tag="PC414_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2464844.1"
                     /translation="MKQFNVLSVCGSGTVTSSMVAGKLKEVLGNKGISITTTEARPTE
                     ALNLAQSGRFDFITFTSPLQTGDYGIPTINAFACLTGIGEEAFFEEVLTVIEGIDK"
     gene            5946..7352
                     /locus_tag="PC414_002457"
     CDS             5946..7352
                     /locus_tag="PC414_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDF2464845.1"
                     /translation="MLEFMKSIIDTFGSAIIVPIIIFIIAKIFKVTTKKSFLSAVYAG
                     VALQGFTLILNSFTPVITPVINRMVESTGVNLPVFDVGWQATSLVAFSTSAGMIYLGL
                     GILLQTILFLIKWTDVFQPSDLWNNYSYMVWGAMVIGVTGNFPLGIACMVLLNLYSLL
                     ISELVAKRWSSYYRYPNCTIIAMHNVEASVFAVFADPIYNKLGLNKIKLNPKELEKKL
                     GFLGEPITLGLFLGMFIGILGNMTRINTMEAWGEIMKVGISTSAVMAIFPKVASMFAQ
                     AFAPITEAARKIMQKAGNREWYIAVNDAVGYGEPATLISGLILIPIMLVIAMVLPGNK
                     VLPVVDLLAIPYMVQGLVAIHNGNIPKVLVSGIIWFGLGLYVCTSTAPLFTDMATNIG
                     VAIPAGAMLITSFNILGKPLMGLVFFAFLSANPIYIGLSVVIYFVLWALFRKNKTSIL
                     DYLEKQALKNVEEEPVAV"
     gene            7696..8754
                     /locus_tag="PC414_002458"
     CDS             7696..8754
                     /locus_tag="PC414_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDF2464846.1"
                     /translation="MGNKMRASVLYNVGDVRYEMVNIPEITDTQVLVNVKYVGICGSD
                     LPRSMVSGLSGNTKYPLILGHEFSGEVVKIGEKVKHINVGDRVAVAPLVPCGKCDYCN
                     EGNFGLCDDYNIIGTRVNGAFAEYVRVPEEHILKLPDTLDYETAAGIEPATIAYHGIS
                     KSNIRVGDSVVVLGCGPIGQFVIQWAKVFGASKIIAVDIFDEKLELSKLLGANYILNS
                     KEVNVIKEIKKITNGGADVVIETAGSRFTQEQSLFVAKKRGNIVFVGISHTELPLSAD
                     ATECILRGELTLKGSWNSYTSPYPGRAWTATLDFMEKGDIIFKPMISDKIGLNEVGDF
                     LSKMSKREINFNKILVEI"
     gene            8853..9539
                     /gene="araD"
                     /locus_tag="PC414_002459"
     CDS             8853..9539
                     /gene="araD"
                     /locus_tag="PC414_002459"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDF2464847.1"
                     /translation="MLENLKEEVLKANLELPKKKLITYTWGNVSGIDREKGLVVIKPS
                     GVEYSNMTVEDMVVVDLEGNIVDGKLRPSSDTPTHLVLYKEFEELGGIVHTHSSWATI
                     WAQIGKSIPSCGTTHADYFYGSIPCTRKMTREEICNEYEKETGNVIIEEFMGRNPIHC
                     PGIIVNDHGPFTWGKDANEAVHNAVVLEEVAKMAYYTELMSPDNIMDKVLMNKHFSRK
                     HGKNAYYGQK"
     gene            9557..10204
                     /locus_tag="PC414_002460"
     CDS             9557..10204
                     /locus_tag="PC414_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HDF2464848.1"
                     /translation="MLDSLKGKVVEIAIKAEHYGLCKEKTGNFSIRDKDTGYILITPS
                     GVGREELKVEHICVLDLDGNVIEVEKGIKPSSEVLMHIEIYKTRENINAISHTHSLYA
                     TAFSVAKKPIIPIVYESVNYGGYVDIASYERPGTLKLAKSVSKLLIDTDACLLERHGL
                     TTVGNDLDEALLKSRYVEEVAEIYYRSLVLNQFKELEVVDVDEFKSWKYPEEIKF"
     gene            complement(10377..11744)
                     /locus_tag="PC414_002461"
     CDS             complement(10377..11744)
                     /locus_tag="PC414_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HDF2464849.1"
                     /translation="MNLIDILNKVDAFIWGPPLLVLLVGTGILLTVKLGVVQITKLPR
                     ALKLIFSAENKGSGDVSSFAALCTALAATVGTGNIVGVATAIKAGGPGALFWMWIAAF
                     FGMATKYSEGVLAIKYRTKDKNGQVSGGPMYYIVNGMGEKWRPLAIFFAISGILVALL
                     GIGTFTQVNSITDAINNSFGIDPRITGVVLAVFVALVVFGGLKSISNVATKIVPFMAV
                     IYVVICGIILISFWNKIPETFMLIIKSAFTPTAATGGFLGATMSLAIRNGIARGVFSN
                     ESGLGSAPIAAAAAKTEWPAEQGLISMTGTFIDTIIICTLTGFSLVISGVWCSDLNGA
                     VMTQAAFNGAIPTFGPILLTVSLTLFAFTTILGWSYYGERCFEFLFGVKGMNGYRTVF
                     VAMVLLGAFLKLEVVWIIADIVNGLMAIPNLIALLALSPIIVSETKKYFEHINSPENQ
                     IKKNA"
     regulatory      complement(11934..12022)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            12450..14570
                     /locus_tag="PC414_002462"
     CDS             12450..14570
                     /locus_tag="PC414_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDF2464850.1"
                     /translation="MGKILVLAEKPSVGRDIAKVLGSKNEKNGYIEGPKYVITWALGH
                     LVTLADPESYGERYKSWSLEDLPILPKHLKTVVIKKSGKQFNTVKSQMNRNDIDEIVI
                     ATDAGREGELVARWIIEKSQVKKPIKRLWISSSTDKAIKDGFAKLRSGKEYENLYYSA
                     IARAEADWIIGINATRALTTKYNAQLSCGRVQTPTLAMLLKREEEIRNFKPKEYYGLE
                     LIATKGNSDIKFIWNDKNNNSSTFSKEKIESTLKKVKGVDIKISEVNKSYKKKYSPAL
                     YDLTELQRDANKMFGFSAKETLSIMQKLYEHHKVLTYPRTDSRYLTSDIVGTLKDRIK
                     AVNISDYSKICNRLLKSNIIANKSFVDNSKVTDHHAIIPTEQKVFMSDFSDKERKIYN
                     LVVKRFLAVLSPPFEYEQTTISATVEGEIFKAKGNKVINLGWKESYKDIEDEDEYESI
                     PNVLEKDVLKVKGLKITSGKTNPPPYLNEGTLLTAMEKNGLGTVATRADIIEKLFNSF
                     LMEKKNKDIYITSKGRQLLDLVPVDLKTPELTASWEKKLLDISKGKLSKNTFINDMKD
                     YSKDIVLEVKNSDNKFKHDNLTKNRCPECGKFMLEVNGKKGKMLICEDRECNTRKTIS
                     QVTNSRCPNCHKKLELRGEGEGRLFTCSCGYREKLSSFNKRKQEEKQKASKKDVNQYL
                     KNQNKNQETFNNPFAEALAKLKNK"
     gene            14674..15927
                     /locus_tag="PC414_002463"
     CDS             14674..15927
                     /locus_tag="PC414_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliS"
                     /protein_id="HDF2464851.1"
                     /translation="MDLSGIFKYYCKECENTWNNSSVELFENIETYSKDSQKKREKEL
                     DKLLNTISVHLERYPSDAVLRKMWVKKGEVFLQKTLEKENIFKLEKMDVEDRKKFLDI
                     TKQFIRDARKFDDDLPIGDIMQAMRNVWISNALQLLFGKEVYYSKANFAYSMLYPYTD
                     NYLDNTNIDKNDKILFNNWLEKRLLGEHIKSKDYHESKVSQMIDYIESVYPREKFTEV
                     YESLLLIFKSQVNSLKQHGKENHLCKEDLLSISIEKGGSSVLVDGYLISGLMTKEEIE
                     FCIGYGFLLQISDDLQDIKEDLKYNHKTIITEMSKEGTLDKVVNKLINFTIELIDSFK
                     INNKNKSVIAMIKNDCLMLILFSVVYNAEFFSVGYIKEVEKFIPYTIDYSLEIEEKIK
                     EKFKNIDVLNNENEYKEMIDIICAE"
     gene            complement(16081..17142)
                     /gene="ytvI"
                     /locus_tag="PC414_002464"
     CDS             complement(16081..17142)
                     /gene="ytvI"
                     /locus_tag="PC414_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HDF2464852.1"
                     /translation="MDIQKQKNFIIKFTYVVLITSIVYIVLKFLLPLLMPFVISFIIA
                     SILRPIIRLITNNTNLNRTLISIVILLIFYGLCIFLLVSFGVKIFASVSDVFFRLPEI
                     YKSSIQPTLNTLFSKIDASTPNVNLALILGWDNISQSMMSLVASISTNALNAIASIAS
                     KTPAFMLKLIITLIASFFFTFDYQKIVNFILKQFPEKSQFMIINIKNSSINALLKLLK
                     AYAILLSVTFIELLIGLTILKVENAFTISVIIALVDILPVLGTGSILTPWMIISLING
                     NINLAIGLLILYIIITVVRQILEPKVVGHQIGLYPLITLMCMFVGAQLFGIAGLFGFP
                     IAATIIKNLHDNGIITAFK"
     gene            17416..18156
                     /locus_tag="PC414_002465"
     CDS             17416..18156
                     /locus_tag="PC414_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HDF2464853.1"
                     /translation="MKNFRDLGGNKTEDGRTVKKGLFYRSAKLSNLSENDIKILKELN
                     IKYIFDYRSDEEARKHPSTIISNIKNIRIPAMRELEESGGSFGSIEDMIDGLFEKDGA
                     FNMLNNSYYNLPINNPSYKKLVELIRDYSNLPILNHCTAGKDRTGVGSAIILMILGVS
                     RENIMKDYLKSNDFADKEIERFIEYKPKFKDIPKENLKYIFGVNEEYMKTAFRRIDEE
                     YISVEAYLYGEFNLNKEEIRKLRNQYLE"
     gene            18168..19946
                     /locus_tag="PC414_002466"
     CDS             18168..19946
                     /locus_tag="PC414_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2464854.1"
                     /translation="MSLNITARQINIIKILLNSKDTISGIALSQEIGCSSKTIQNEIK
                     DINKQLRNGKILSIRGVGYKLEGSFDEVNLNSNLYDDVDRVEYIIRKILTLSNEEKNT
                     IKLEDLADSMYVSVSTVKNDLKEVKRMLEKYSISVKSKHKQGICVLESEDKILNCIVD
                     LSNKRDNQLSLNDFLNDDIKERKLLIKKLLLDILNENGLVLTDIEFKSVLNNILVMLS
                     RHKYNEKEFIENYIENYRSKRSSILENEQNKEIIINSIKTFCKNLKLATSIDISNDKV
                     FEEFLYKHISSLCKKIKLGINQSPIMAQDIKIKYPFAFELANIAKKTIEKDLKIEINE
                     DEVANIALHIGGAVERASYNNKDKVFKTIIVCTSGIGTSMLIKAKLENIFKEKLEILK
                     IIPSYLVEYIKVIDVDFVISTVPIEVGDVPVINISPILSEKEIRLIEKYIETGNIYLD
                     LDIKSLFDSELFFTDLVFKRKEEVIDFMASKLVQKGYIDEDMKKSYFEREKIATTEIG
                     NMVTIPHGAIGKIFDNKIAIGILKKPISWEISDVRLIIMLALDKDKILDYEVIFSKIY
                     KRVDSIAKVISICENSSFEKFVNLFN"
     gene            20113..21030
                     /gene="pfkB"
                     /locus_tag="PC414_002467"
     CDS             20113..21030
                     /gene="pfkB"
                     /locus_tag="PC414_002467"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDF2464855.1"
                     /translation="MIYTVTLNPSIDYIVKLDELKTGSTNRVKEEYVYPGGKGINVSR
                     IFKELGNDNTSLGFISGFTGEYIIRTLEEKELKTDFIKIKSGFSRINVKIKALKETEI
                     NGQGPNIDDEDIDTLYKKLDKLNQDDILILAGSIPSTLDEKLYENIMARLEKKNIKVV
                     VDATKNLLLNVLKYKPFLIKPNNDELEELFGVKLNSIEDMVKYARRLKEMGAINVLVS
                     MGKDGALLITEEDEVLISDVPKGKVKNSVGAGDSMVAGFISGYLNTGKYDYALKLGAA
                     SGSATAFSYDLAKREYIDKLVNEISVKQF"
     gene            21067..22986
                     /locus_tag="PC414_002468"
     CDS             21067..22986
                     /locus_tag="PC414_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2464856.1"
                     /translation="MKIIDLLDEKSIKLNLNSKTKSEAIEELVDLVANSGNLNDKENY
                     KKAILAREEMSTTGIGEGVAIPHAKNSSVTKACIAAAVSKEGIDYESFDGSLSNLFFM
                     IAAPDGANNTHLEVLSRLSTILMDEDFRNKLINSSSEKEFKEIIDKKEREKFSEEYQD
                     EKVTEKNIIDTKENDANKYPKVLAVTACPTGIAHTFMAAESLNKMAENKGVSIKVETN
                     GSAGVKNKLTKDEIENATCIIVAADKNVEMARFNGKKVIKTKVADGIHKAEELIDKAV
                     NGDAPIYHGGDGSHNESNEESESGFRKVYKHLMNGVSNMLPFVIGGGILIAIAFLLDD
                     YTINPSNFGSNTPIAAFFKGIGDKAFGFMLPVLAGYIAYSISDRPAFVVGFVGGALAG
                     DGGSGFLGALLAGFIAGYLVEGLKKIFSVLPASLEGIKPVLLYPLLGTLLMGIIMTFL
                     VIPPVTAINNAMVGFLNGLGGTSKIVLGLVLGGMMAVDMGGPVNKAAYVFGVASLESG
                     QFEIMAAVMAGGMVPPLAIALATTFFKNRFTKEERDSGKVNYVMGLSFVTEGAIPFAA
                     GDPLHVIPACVGGSAVAGALSMLFNAALRAPHGGVFVIPVVTHPFAYILAIAVGALVG
                     MMLLALLKKPLNQEV"
BASE COUNT         9244 a         2509 c         3932 g         7527 t
ORIGIN      
        1 ataaacatca ttctgactac tattaaaaga catataaaat aattaaactt aaaagtatat
       61 ttgcttatct ttttagtaaa taaaatgaaa aaaagaatta gtggacttat gtaaatatta
      121 aatttaataa cttctataaa taattccata aaatacgtct cctattttct tttttttagc
      181 cattgttcaa gttcttttat gtcatcatct gatatttcat ttccacaacc taatgatgat
      241 ataaagctat gaatagaatt tttatgtata agctctaaaa aatcctttgt ttcaaatttt
      301 agatattcct catgtttaac tttaatagtg tataaagtat ttctaccatc tttttcaaat
      361 tctaaaaaac ctttatcact taatctagaa agtactttac cagtagtacc ttttgtccat
      421 ccatgagaaa tttccatata attagctact tctctggctg gtattttatc tatgtcttta
      481 tcccatatga aattcataac taacaactcg gattttggta tttttttcaa tataatcact
      541 cacctttaac ttgattatat tagacaaatg tctaatagtc aatataatat atcatcatgt
      601 ataaataatc tttatattat tttatgcttg agaactaagt agtatagatt tatagataaa
      661 aaagatattg agagttttat ggaagagggg aatatatttt ctattagaga taagaatatg
      721 acatatattt tttattgatt tttacaaaaa tactatatag tgaataaatt aatatctttt
      781 ctaaattaga agaggagaat cttattagat aaagattatc ctctttattg aaagtttaag
      841 ggatattaga gattattaat ttttctctct ttttttaact aaaagcaata atacataaaa
      901 ttattattgc taatgatact cttaatgtgg tatatagtca tagtgtcaca tgtttatcaa
      961 tagttttttc attaattatt gtaacgaata gtaggactca taaaatactt agaaatatgt
     1021 ttttagatag attttaaata tataatctaa cttatatcta taaattaaca ctattattct
     1081 ccaattccat ttctagtttt tttataaatt tattaaattc ttctggttgc ctttttccaa
     1141 acaatactct tacaagagct gaattgattt ttccaaatat attcctcttg ctctcacttt
     1201 caataaaaat aagttttgta ttttctaagt caatatgtac taattcatat ctagtaatag
     1261 tactttctct tttattgctt gtttttatct catatactct atttttttcg aaattagtta
     1321 tttcaacctt aaatctttta ttattttttt gctgtacaaa tgttcctact gcttttttct
     1381 cattaaaatt aggaaaattt agtcttgcag attttgtaaa tatattaaat atgtcttttg
     1441 cagaatattt taataaaact tcagttttaa atgtattcat atcgttttcc cttctgtata
     1501 taaatttaat atttataagt taatttttaa gttaaaatag tataatatgc catattaaat
     1561 catgttaaaa ctatttatat agctaaaatc ttacctatat aagtatgtat actatcatat
     1621 aaaaataaaa tcttaaaata gaaagggtta aaaacacagt ttattataat ttttattgtt
     1681 gagtagatac gtactattat atatgattga gcaagttttt caatcataaa tttataaagt
     1741 attttgcatt aaaatttaaa caaagaatta aattatttaa gattatgaat aactaataca
     1801 tttagtaaat tactaaatat acaaaatgta aatacttatc aaaatgtata aatacttatg
     1861 acatctagtt acttacaggg tttaagtaga aaaaaatatc aaagtaagta tggcatgata
     1921 gttgcttata tatttatcaa gaaaggagtt attaagcatg gagttgagag ataaacttgt
     1981 aaacatcata aatacagaaa ataaaaaaga tccattaaca gatgttcaaa tagcaaagtt
     2041 tttaagtaca actagagaga acataactaa tttgagaaaa gaacttaata taagtaattc
     2101 aaggcaaaga agatatcctt atttaaaatc agcaatttcg gcaatattac aaaagaataa
     2161 gaatatttct atatcagaaa ttacaaggga attgatgaca gaaggcttta atatatcgag
     2221 aagagttgtg gaggaacttt tacctaaaga aagtttagaa atggatgtag tagaggaaga
     2281 tgaagaagaa tcgaatgacc catttgaaac tctgataggt aataaaggga gtcttagaaa
     2341 tgctgtagag caggctaaat ctgctatatt gtatccacca aaagggttac caacgttgat
     2401 tattggggaa agtggagttg gtaagagttt attttctaga catatgtatg agtttgcaaa
     2461 gcaaaaaaag atagtaaaag agagctccaa ttttgttgta tttaactgtg ctgattatag
     2521 tgacaatcca caattattat tatctttgtt atttggatat aaaaaaggtg catttacagg
     2581 tgctgaatat gatacaccag gtcttgtaga agaagctgat gaaggagttt tatttttgga
     2641 tgaaatacat agattgcctc caaaaggtca agaaatacta ttttctatct tagatagggg
     2701 aaagtttagg agattaggtg aaactaatgc tgaaagaaaa gtaagtatta tgttgattgg
     2761 ggcaactact gaaaatgtag agactaattt attattaaca ttcagaagaa ggataccaat
     2821 gctaataact ttgccaccat tacatgatag attactaaaa gagaaaatag accttatata
     2881 caatatcttt caacaagagt gtaatagaat aaatgcaaaa atatttgtag ataaaaatgt
     2941 gattgaaata cttgcactaa aaaagtttag tggaaatata ggacaattac aaaatatgat
     3001 tcaggttctt tgtgcaagag cttttatgaa gtttataaat tccaaagatg aagatagtga
     3061 atctatagta gtagtagata taaatgaagt tttaaagctt aaagattcgt ttaaagatgc
     3121 tgcctttcaa gagattgaat atacagagat aagaaaatat ttgaaaaatg ctatatttat
     3181 accatttaat ttagaagaat catcagtaaa aggaagtctt ttaagtgatg aatatatatt
     3241 accagaggac atatacaaaa caattgaaaa gaaatattat gacctaaaat ccctagacat
     3301 tgaagatata gatatagaga atattttatg gacttttatt ttaaatagat ttgctgactt
     3361 aaagtttaat cttgatagta atgaagaaat tttttcaatg agtgatttaa atagttttgt
     3421 aagtgatagt ttagttaaac tggtaaaaga acttatgaat gatatagttg aaaaaaatat
     3481 gaatttagaa gtaaataaaa atatgttcaa atatctagca atacacttag aagaagctat
     3541 aaaaagaatt aagctaaatc aaaagataat aaatgtaaat ttagaaaaaa taaagattga
     3601 cttttcaaaa gaatatgaaa ttagtaaaag ttttgcaata aaaattgagg aatcaattgg
     3661 tattaagatt tcagatgatg aaattggatt tataacgctc tacataaagt ctgctctaaa
     3721 aaatgaagtg aaaaaagata aagtagggtt aataataatt tctcatggac gtatagctac
     3781 agagactgta aatgtagtta aagaactttt aggtgtaaag tttcctgtgg ctatagatat
     3841 gcctttagat gaaaaaccaa taaatatata caataaggca gtagaattgt caaaaataat
     3901 agaccaaggg aaaggcatat tgtttttggt ggatattggt tcacttacaa acataggtca
     3961 aatagtaaat aagagaacca atataagtac taaaactatt gatagagtag acctcctaat
     4021 ggcactagaa gcaactagaa aagtttctat gggagaggaa gaacttgatg aaatattttt
     4081 ttctttgcta aaagatagga tgggatataa ctatgaatta gaaaaacaca tagataaatc
     4141 aaatgcaatt ataacattat gtcttactgg agaaggaact gctaagtata taagtaaaac
     4201 gttggaagaa aagtatgata acactaaatg ttatcaaatg agtgcattgg atgaaagttt
     4261 attttgtaaa attgaaaatc taaaagagac aaacaatata ttagctattg ttgggacaat
     4321 aaaccctaag ataccaggta taaattttat accctataat aaagaacttt tcaaaaattt
     4381 ggatatctat ctatcaaaat caaaacaaca agatgacaga ccaattaaat cgtatgaaag
     4441 aatgcttgat gaggatttag tgatttttga accagatata tattttaaaa aggatttatt
     4501 agaatatgta tgttcaattc ttataaataa agaatatgta gaaaaagatt acttagattc
     4561 tgttttacat agagaagaaa tgcttccaac atattctaaa ggagctatag gtgtgcctca
     4621 tggagattct tctactgtaa atagtacaag gtttgttttt gttaaactaa agaatcctat
     4681 tgattggggt gtaggtaatg taaatttcat atttatgcca gtttttcagg ctaatgataa
     4741 agagattgtt aaaaatgttc ttgagatact aaaagatgca gaatttatga acaatgccaa
     4801 taagtgtttt gataaagatg cttttagaag gattatattt gataaattta aacatctgta
     4861 gccatagcaa aaaaattgag aggagttaga gtaatgaatg tagatttaat atcattgggt
     4921 ttagaatcta atgattatga aagtgttatt gaagaattag gaagtataat gtgtaaaaaa
     4981 gaatatgtaa aagaaacata cataaatgct gtcttggaaa gagaaaggac attaccaaca
     5041 ggtctagata ttggagacat gtgtgttgct attccacata cagattcaaa gcatgtaaat
     5101 gagtctaatg tagctgtagg gatattgaaa aatcctgtaa agtttaattc aatgatagac
     5161 ccaaaagata gacttgatgt agaacttgta tttttattag cagtaaagaa cccagactca
     5221 caagtaaaac tgttaaaaga tttaatgtca gtttttcaaa atataaagtt attaaaaaat
     5281 ataaaaaacg catcaacaaa agaagaagta gctaaattat tggattttat agaaatttaa
     5341 tgtaggagaa ttttataaat aaaaaattaa aatttaaaca atcaaagtaa gtattatttt
     5401 tttaataggt ttaatcttat ataaaataaa aatttagagt gaaaaattag gaggaaatta
     5461 ttatgaaaca atttaatgta ttatcagttt gtggttcagg gacagttaca tcatctatgg
     5521 tagcaggtaa attaaaagaa gtactaggta ataaggggat ttctattact acaacagaag
     5581 ctagaccaac agaagcacta aatttagcac aatcaggaag atttgacttt ataacattta
     5641 cgagtccact tcaaacaggc gattatggaa taccaactat aaatgcattt gcttgcctaa
     5701 ctggtatagg ggaagaggca ttctttgaag aagttttgac tgttattgaa gggatagata
     5761 agtaagtttt ggagacgtga atttatagta tttaaatcaa tgatttagat actataataa
     5821 gctaatatta actaaatatt tgtggaaaaa acattttata tgttgtcata aattaaaaaa
     5881 ttataaaaat agtcacttaa gtattaaatt aagcttataa atatttataa gtaggaggga
     5941 aaaagatgtt agaatttatg aaatctataa tcgatacatt tggttctgca attatagttc
     6001 caattataat attcataatc gcaaagatat ttaaagtaac tactaagaaa tcgtttcttt
     6061 ctgctgtgta tgcaggagtt gctttacaag gttttacact gattttaaat tcatttacac
     6121 cagtaataac accagttatc aataggatgg ttgagagtac aggagtaaac ctaccagttt
     6181 ttgatgttgg ttggcaagca acatcattgg ttgcattttc aacaagtgca ggtatgatat
     6241 atttaggtct tggtatatta ttacaaacaa ttttattttt gataaaatgg acagatgtgt
     6301 ttcaaccatc agatttatgg aataactatt cttatatggt atggggagca atggttatag
     6361 gagtaacagg aaattttcct ttaggtatag catgtatggt tttattaaac ctatatagtt
     6421 tattaatatc agagttagtt gcaaagagat ggtctagtta ctatagatat cctaattgta
     6481 caataatagc tatgcataat gttgaggctt ctgtgtttgc agtatttgca gaccctatat
     6541 acaataaatt gggattaaat aaaataaaat taaatccaaa agaattagag aagaaattag
     6601 ggtttttagg tgaaccaatt actttaggtc tttttttagg tatgtttata ggtatattag
     6661 gtaatatgac gagaataaat actatggaag cttggggaga aataatgaaa gtaggtatat
     6721 ctacaagtgc agtgatggca attttcccta aagtagcaag tatgtttgct caagcatttg
     6781 cacctataac agaagcagct agaaagatta tgcaaaaagc tggaaaccgt gagtggtaca
     6841 tagcagtaaa tgatgctgtt ggatatggtg aacctgcaac tttaatatca ggtcttatat
     6901 taattccaat aatgcttgta atagctatgg tacttcctgg taataaggta ttaccagttg
     6961 tggatttatt ggcaattcca tatatggttc aaggtttagt tgccatacat aatggtaata
     7021 taccaaaagt attagtttca ggtataattt ggtttggatt aggattatat gtttgtacat
     7081 ctacagcacc attatttaca gatatggcta caaatatagg tgtagctata cctgcaggag
     7141 caatgttaat aacaagtttt aatatactag gaaaaccttt gatgggatta gttttctttg
     7201 catttctaag tgcaaatcca atttatatag gtctttcagt tgtaatttac tttgttctat
     7261 gggcattgtt tagaaagaac aaaacaagta ttcttgatta tttagaaaaa caagcactta
     7321 aaaatgtaga agaagagcct gtagcagttt aagtttataa aaatgaaata aaaataatta
     7381 aaggagttta taattgattt tatataagta tcagataata cctgagaaat atttaaatga
     7441 aatatagact tctttttatt tgataatatt tgtaattata atgcattgta tttttatagt
     7501 tgagttttct gtataacctg ctatttaaaa catactcttt tatatttata cagtaatgtg
     7561 gtttaatttt ataaaatgta atctctactg gtaaaagact ttgtatataa ggcatttgcc
     7621 tgaataaacc atttatcttt tgtatggcat agatattgct atatagaagt atatagcatg
     7681 aaaggagata tttatatggg aaataaaatg agagcatcag tattgtataa tgtaggtgat
     7741 gtcagatatg aaatggtaaa tataccagaa ataacagata cgcaagtgct tgtaaatgta
     7801 aaatatgttg ggatatgtgg ttcagattta cctaggtcta tggtaagtgg tttgagtgga
     7861 aatacaaaat atccattgat tttagggcat gaattttctg gtgaagttgt taaaattgga
     7921 gaaaaagtaa agcatattaa tgtaggagat agagtagctg ttgcaccatt agtaccttgt
     7981 ggaaaatgtg attattgtaa tgaaggtaat tttggcttgt gtgatgatta caatataatt
     8041 ggaacaagag taaatggagc gtttgcagaa tatgtaagag ttccagaaga acatatttta
     8101 aaactaccag atacattgga ttatgaaact gctgccggaa ttgaaccagc aactatagct
     8161 tatcatggga tttcaaagtc taatattaga gtaggagata gtgtagttgt gttaggatgt
     8221 ggtccaatag gtcagtttgt tatacaatgg gcaaaggtgt ttggtgcatc taagattata
     8281 gcagttgata tttttgatga aaaattagaa ctttctaaac ttctaggagc aaactatata
     8341 cttaactcaa aagaagtaaa tgtaatcaaa gaaataaaaa aaataacaaa tggtggtgct
     8401 gatgttgtaa tagaaactgc tggtagccga tttacacaag aacaatctct atttgtagca
     8461 aaaaagagag gaaatatagt gtttgtaggt atatcacaca cagaattacc acttagtgca
     8521 gacgctactg agtgtatatt acgtggagaa cttaccttga aaggttcttg gaattcatat
     8581 acaagccctt atcctggaag agcatggact gcaacattgg attttatgga aaaaggtgac
     8641 attattttta aacccatgat ttcagataaa ataggtctta atgaagtggg tgacttttta
     8701 agtaaaatga gcaagagaga aattaatttc aataaaatcc tagtagaaat ataaaaattt
     8761 aataaaaaat tctaaaagca attaaatttt ataactatga cattaaataa aataaaaata
     8821 attttcttaa ataaaatata ggaggaagaa aagtgttaga aaatttaaaa gaagaagtat
     8881 taaaagcaaa cttagaatta cctaagaaaa agctaattac ttatacttgg ggaaatgtaa
     8941 gtgggataga tagagaaaaa ggattagtgg tgataaagcc aagtggtgta gagtatagta
     9001 atatgactgt agaagatatg gttgtagttg atttagaggg aaatatagtt gatggcaaat
     9061 taagaccttc ttcagatacc ccgactcatc tagttttata taaagagttt gaagaattgg
     9121 ggggaatagt acatacacat tcaagttggg caacaatttg ggctcaaata ggaaaatcta
     9181 taccatcttg tggtactact catgcagatt atttttatgg aagtatacca tgtaccagaa
     9241 aaatgactag agaagaaatt tgcaatgaat acgaaaagga aactggaaat gtaatcattg
     9301 aagaatttat gggaagaaat ccaattcatt gtccaggaat aattgtaaat gaccacggac
     9361 cttttacatg gggaaaagat gcaaatgagg ctgtacacaa tgcagtagtt ttagaagaag
     9421 tcgcaaagat ggcttattat acagagttaa tgagtcctga taatattatg gataaggttc
     9481 ttatgaataa gcatttttca agaaaacatg gaaaaaatgc atactatggt caaaaatgat
     9541 tatctggagg tgaattatgc tagactcttt aaaaggtaaa gttgtggaaa tagcaattaa
     9601 agcagagcat tatggtttat gtaaagaaaa gactggtaat tttagtatta gagataagga
     9661 tactggatat atacttataa ccccatcagg tgtaggaaga gaagaattaa aagttgaaca
     9721 tatttgtgta ttggatttgg atggaaatgt aatagaagtt gagaaaggta ttaagccaag
     9781 tagtgaggta ttgatgcata ttgaaatata caaaacaaga gaaaatataa atgccatatc
     9841 acatactcat tctttatatg caacagcatt ttcagtagca aagaaaccaa ttataccaat
     9901 tgtatatgaa tcagttaatt atggcggtta tgtagatatt gcttcatacg aaagaccagg
     9961 aactttaaaa ttagctaaga gtgtttcaaa attactgata gatacagatg catgcttatt
    10021 agaaagacat gggttgacaa ctgtaggtaa tgatttagac gaagcattat taaaatcaag
    10081 atatgtggaa gaggtagcag aaatatacta taggtcttta gtattaaatc aatttaaaga
    10141 acttgaagta gtagatgttg atgagtttaa atcttggaaa tacccagaag aaataaagtt
    10201 ttaatctaaa taattttcat tgaagtggtt aattaaaata tatgtattat ttatatcaat
    10261 tactatgttt tattatgtta attaccgtat tttgcaaata tatttattac attttttcaa
    10321 aaaaatatag aatccgaaag ccatataaaa ttgacttggg attctatact ttttatttat
    10381 gcatttttct ttatctgatt ttctggagaa tttatatgtt caaaatattt tttagtttca
    10441 gatactatta taggtgataa tgcaagtaaa gctataaggt taggtattgc cataagccca
    10501 tttacaatat cagcaattat ccaaacaact tctaacttta agaaagcacc taataacacc
    10561 atagccacaa atacagttct atacccattc atgcctttta caccaaacag aaattcaaaa
    10621 catctttctc cataataact ccatcccaaa attgtagtaa atgcaaataa tgttaaacta
    10681 actgtaagta atattggtcc aaatgtagga attgctccgt taaaagctgc ttgtgtcata
    10741 acagctccat ttaggtctga acaccacact cctgaaataa caagagagaa tccagtaaga
    10801 gtacagatta taatagtatc tataaaagta cctgtcatag atataagacc ttgttccgct
    10861 ggccattcag ttttagcagc agctgcagct attggagcac tacctagacc agactcattt
    10921 gaaaatacac cacgagcgat accatttcta attgccagtg acatagttgc tcctaaaaaa
    10981 ccaccagtag cagcagtagg tgtaaatgca cttttaatta ttaacataaa agtttcaggg
    11041 attttattcc aaaaagatat taaaatgatt ccacagatta caacatatat aactgccata
    11101 aaaggtacta ttttagtagc tacattagat atacttttaa gtcctccaaa tacaactaaa
    11161 gctacaaata ctgctaatac aacccctgta attctagggt ctatcccgaa actattattt
    11221 attgcatctg ttattgaatt tacttgagta aaagtaccga ttcctaaaag tgcaactagt
    11281 ataccactta tagcgaaaaa tattgcaaga ggtctccatt tttctcccat accatttact
    11341 atgtaataca ttggcccacc agacacttga ccatttttat ctttagttct gtattttatt
    11401 gcaagaacac cttctgaata cttagtagcc attccaaaga atgctgctat ccacatccaa
    11461 aaaagtgcac caggtccacc agctttgata gctgtagcaa caccaacaat attaccagta
    11521 ccaactgtag cagctagagc agtacataag gctgcaaaac ttgaaacatc accactacct
    11581 ttgttttcag cagagaatat taacttaaga gctcttggca gtttagttat ttgaacaact
    11641 ccaagcttaa cagtaagtag tataccagta ccaactaata gtaccagtag gggtggtccc
    11701 caaataaaag catcaacttt gtttaaaata tcaattagat tcataaaaac actccctcat
    11761 tttttttgct ttacataatc aatagaattt ttaatgaagt aattgacagg aaataagtat
    11821 aatttattaa taatttctag tgtaataaaa aaagctaaat ctaaaaaaga ttcagctctg
    11881 gatgtacact aattagcaac caaaataaaa gtattagtaa atactgtttt tggtagataa
    11941 tccctgtcct tttgcctgag agattgagca taaaagcctt gccccttcgg cgcccataat
    12001 gggtctctcc agagttccgt aaattttaca gtcctacttt taaaagtacc tgagagtgtt
    12061 actccttcgg tgtgctatat agcaactctt ctataaaatc ttaaccggat attgatttgt
    12121 catagcttat tgtattaata atattaatat aagtttttat gttttgtcaa ctaaaaaatt
    12181 aaaaaacaaa gaaaaaatat aaaaatgtaa gcctatttac gaattataaa cttagaaaga
    12241 tgagtaatat acgtgaaaat gaattttgta atacacatac aaatgaagtg taaaataagt
    12301 tttaaataaa aaaataataa aatcttaaat aattaattag atatgattaa attatggtaa
    12361 tattatagtt acgatattaa gaaaagttct atttagttag attttaagcg taatatgaga
    12421 ttatgtatag agaaaggatg tatttttaga tgggaaaaat attagtgcta gcagaaaaac
    12481 cttctgttgg aagagatata gcaaaagttt tagggtcaaa aaatgaaaaa aatggttata
    12541 tagaggggcc aaagtatgtg ataacttggg cactaggtca tttggttaca cttgctgacc
    12601 ccgaaagtta tggagaaaga tataaatcat ggagcttaga agacctgcca atacttccca
    12661 agcaccttaa gactgttgta ataaaaaaga gtggaaagca gtttaataca gttaaatcgc
    12721 aaatgaatag aaatgatata gatgaaatag ttatagcaac agatgcaggt cgtgaaggag
    12781 aacttgtagc aaggtggata atagaaaaat cacaggtaaa aaaacctata aaacgtcttt
    12841 ggatttcttc aagtacagac aaagcaataa aagacggatt tgcaaaatta agaagtggaa
    12901 aagaatatga aaatttgtat tactcagcaa tagcacgagc agaagctgat tggattattg
    12961 gaataaatgc aaccagagct ttgacaacaa agtacaatgc acagctttct tgtggaagag
    13021 tacaaactcc tactcttgca atgttgctta aaagagaaga agaaataaga aatttcaaac
    13081 ctaaagagta ttatggatta gaattaattg ctacaaaggg aaattcagat ataaaattta
    13141 tttggaatga taaaaacaat aattcttcaa cttttagtaa agaaaagata gaatccacat
    13201 taaaaaaagt aaaaggtgta gacataaaaa tatctgaagt aaacaagtca tataagaaaa
    13261 aatattcacc tgcactctat gatttaacag aattacaaag agatgcaaat aaaatgtttg
    13321 gattttcagc aaaagaaact ttatctataa tgcaaaaact gtatgaacat cataaagtac
    13381 ttacttatcc tagaactgat tctagatatt taacatcaga tattgttgga acattaaaag
    13441 atagaataaa agctgtaaat attagtgatt attcaaagat ttgtaataga ttattaaaaa
    13501 gcaatattat agctaataaa tcgtttgttg acaattcaaa ggtaacagac caccatgcaa
    13561 taataccaac agaacagaag gtattcatga gtgattttag tgataaagag cgaaaaatat
    13621 ataatcttgt agtaaaaaga tttttagcag tactatctcc tccttttgaa tatgagcaga
    13681 caactataag tgcaactgta gaaggagaaa tatttaaggc taagggaaac aaagtaataa
    13741 atttaggttg gaaagaaagt tataaagata ttgaagatga agatgagtat gaaagtatac
    13801 caaatgtttt agaaaaagat gtacttaagg tcaagggatt aaagataact tcaggaaaaa
    13861 caaatccacc accatactta aatgagggaa cattattgac tgctatggaa aagaatggtc
    13921 tagggacagt tgctacaaga gcagatataa tagaaaaatt attcaattca tttttaatgg
    13981 aaaagaaaaa taaagatata tatataacat caaaaggaag acaattatta gatttagttc
    14041 cagtagactt aaagacacca gaacttacag cttcatggga aaagaaatta ttggatattt
    14101 caaagggaaa acttagtaaa aatacattca taaatgacat gaaagattat tcaaaagata
    14161 ttgtactaga agtaaaaaat agtgataata aatttaaaca tgataactta actaaaaata
    14221 gatgtccaga atgtggtaaa tttatgttag aggtaaatgg taaaaaaggt aagatgttga
    14281 tttgtgaaga tagagagtgt aatactagaa aaactatatc acaagtaacc aattcaagat
    14341 gtcctaactg ccataaaaaa ttagaacttc gtggagaagg tgaggggaga ctcttcactt
    14401 gtagttgcgg atacagagaa aagttgtctt ctttcaacaa gagaaagcaa gaagaaaaac
    14461 agaaggcatc aaaaaaagat gttaatcaat atttaaaaaa tcaaaataaa aatcaagaga
    14521 catttaataa tccatttgca gaagcattag ctaagctaaa aaacaaataa ttttaaagtt
    14581 attattatac acaattataa ctttaactgt atattagtta tatgtactga tataatatag
    14641 tgtatagttt aggcatgaaa gtagggctgt taaatggatt taagtggcat atttaaatac
    14701 tattgtaaag agtgtgaaaa tacatggaat aattcgagtg ttgaattatt tgagaatata
    14761 gaaacgtata gtaaagattc acaaaaaaag agggaaaaag aattagataa attgctaaat
    14821 acaatatcag ttcatttaga gaggtatcca agtgatgctg tattgagaaa aatgtgggta
    14881 aaaaagggcg aggttttctt acaaaagaca ttggaaaaag aaaatatttt taagttagaa
    14941 aaaatggatg tagaggatag aaaaaaattt ttagatataa caaaacagtt tattagagat
    15001 gctagaaaat ttgatgatga tttacctata ggtgatatta tgcaagctat gaggaatgta
    15061 tggatttcaa atgcattaca attattattt ggtaaagaag tatattattc aaaagctaac
    15121 tttgcatata gtatgttata tccatatact gataattatt tagacaatac aaatatagat
    15181 aaaaatgata agattttatt taataactgg ttagaaaaaa ggctcctggg agaacacatt
    15241 aaatctaagg attatcatga aagtaaagta tctcaaatga tagattatat tgaaagtgta
    15301 taccctagag aaaagtttac agaagtttat gaatcgttat tattaatatt taaaagtcaa
    15361 gtaaatagtt taaaacaaca tggtaaggaa aatcatttgt gtaaagaaga tttattatcc
    15421 atttctattg aaaaaggagg ttcatccgtt ttagtagatg gatatttaat aagtggattg
    15481 atgacaaagg aagaaataga gttttgtata ggatatggat ttttattaca aatatctgat
    15541 gatttacaag atataaaaga agatttaaaa tacaaccata aaactattat tacagagatg
    15601 tcaaaagagg gtactttaga taaagttgta aataaactaa taaattttac tattgagtta
    15661 atagatagtt ttaaaattaa taataaaaat aaatctgtaa tagctatgat aaagaatgat
    15721 tgcttaatgt taattttatt ttctgtagtt tataatgctg aatttttttc tgtaggatat
    15781 ataaaagaag tagagaaatt tattccatat acaatagatt attcattaga gattgaagaa
    15841 aaaataaaag aaaaatttaa aaatatagat gttttaaata atgaaaatga atataaagaa
    15901 atgattgata ttatttgtgc agagtagaaa aaaagggagt gtcttagaat agacttttta
    15961 gtttatgagg cactcttttt gtataaaatc aaatgtattt tcatcatttt aacaattaaa
    16021 aagagcttat ccctatttga gaaagccctt tttaatttaa ataaaaatgt caaggttaaa
    16081 ctatttaaat gcagtaataa tcccattatc atgtaagttt tttatgattg tagctgcgat
    16141 agggaatcca aataatccag ctattccaaa tagttgagct ccaacaaaca tacacattaa
    16201 tgttattaaa ggatataggc caatttggtg tcctactacc tttggttcta atatttgtct
    16261 aacaacggtt ataattatgt atagtattaa aagtcctatt gctaaattaa tatttccatt
    16321 aatgagtgag ataatcatcc aaggtgtcaa aatacttcct gtacccaaga ctggcaaaat
    16381 atcaacaagt gctatgatga cagatatagt aaatgcattt tcaaccttta ggatagtaag
    16441 tccaataagt agttctatga atgtcactga tagtagtatt gcataggctt ttagtagttt
    16501 taaaagagca ttaatactgc tattttttat atttattatc ataaattgac ttttttctgg
    16561 aaattgtttt aagataaagt ttactatttt ttgatagtca aaagtaaaga aaaaagaagc
    16621 aattaaagta ataataagtt ttaacataaa tgcaggtgtt ttacttgcaa tactagcaat
    16681 agcattgagt gcatttgttg aaatagaagc tactaaagac atcatagatt gacttatatt
    16741 atcccaacca agtattaatg ctaggttaac attaggtgta ctagcatcta tctttgaaaa
    16801 taaagtattt agcgttggtt gtatacttga tttatatatt tcaggaagtc taaaaaatac
    16861 atcacttaca cttgcaaata tcttcacacc aaagctaact aataaaaata tacacaaccc
    16921 ataaaaaatt aaaagtatta ctatagaaat aagtgtacga tttaaatttg tattatttgt
    16981 aataagtctt ataataggtc ttaagatgga tgcaatgata aaactgatta caaaaggcat
    17041 taataatggc aataagaatt ttaatacaat atatacaata cttgttatta aaactacata
    17101 tgtaaattta attataaaat tcttttgctt ttgaatatcc ataatataaa acctccaatg
    17161 tatttgatat tattctaaat atggtctttt ttacagaaaa tatactactg tatattgatt
    17221 ataaactata aagcttatgt aaagtaaata aattatgtaa aatttaggtt aattttgtat
    17281 aaagagatac ttttatattt tacaattaat aaccaaatac taaaaaatat tttaaataaa
    17341 gaacttaaaa ttgtgataaa attaatttat agatattaaa aaaagtataa tatatggttt
    17401 ttaagaggtg ctaagatgaa aaattttaga gatttaggtg ggaacaaaac tgaggatggc
    17461 agaactgtaa agaagggatt attttatagg tctgcaaaat tatcaaatct aagtgaaaat
    17521 gatataaaga ttttaaaaga gttaaatatt aaatatatat ttgattatag aagtgatgaa
    17581 gaagctcgta aacatccatc aacaataata tcaaatataa aaaatataag aataccagca
    17641 atgagagagc tagaagaatc aggaggaagt tttggttcta ttgaagatat gattgatgga
    17701 ttgtttgaaa aagatggtgc atttaatatg ttaaataata gttactataa tttgcctata
    17761 aataatccgt catataaaaa attagtagaa cttataagag actattctaa tttaccaata
    17821 ttaaatcatt gtacagctgg taaggataga actggtgtgg ggagtgctat aattcttatg
    17881 attttagggg tatcgagaga gaatataatg aaagattatc taaaaagtaa tgattttgca
    17941 gataaagaga tagaaagatt tatagaatat aagcctaaat ttaaggacat tccaaaagaa
    18001 aatttgaaat atatatttgg tgtaaatgaa gagtatatga aaactgcatt tagaagaatt
    18061 gatgaagaat acattagtgt agaagcatat ttatatggag aatttaattt aaataaagaa
    18121 gaaataagaa aattaagaaa tcaatatcta gagtaaaagg tgagtttatg agtttaaata
    18181 taacagcaag acagataaat ataataaaaa tattgttaaa ttcaaaagat acaattagtg
    18241 gtattgcctt aagtcaagag ataggatgtt cttcaaaaac tatacaaaat gaaataaagg
    18301 atattaataa acaacttaga aatggtaaaa ttttgtcaat aagaggagtt ggttataaac
    18361 tagaaggaag ttttgatgag gtaaatctaa atagcaatct ctatgatgat gtagatagag
    18421 tagaatatat aatcagaaaa atattaacct taagtaatga agaaaaaaat actataaaac
    18481 ttgaagattt agccgattct atgtatgtaa gtgtttcaac agtaaaaaat gatttaaaag
    18541 aagtaaaaag aatgttggaa aagtattcaa tatcagttaa atctaagcat aaacaaggta
    18601 tatgtgtttt ggaaagtgaa gataagatat taaattgtat agttgactta agcaataaga
    18661 gagataatca attgagttta aatgattttt taaatgatga tataaaggaa agaaaactat
    18721 taataaaaaa attattatta gacatattaa atgaaaatgg attagtatta actgatatag
    18781 aatttaagag tgtgttaaat aatatattag ttatgttaag cagacataag tataatgaaa
    18841 aagaatttat agagaattat atagaaaatt ataggagcaa aagaagctca attttagaaa
    18901 atgaacaaaa caaagaaatt ataataaatt caataaaaac attttgtaaa aatttaaaat
    18961 tagctacatc aatagatata agtaatgata aggtatttga agagtttttg tataagcata
    19021 ttagtagttt atgtaaaaaa ataaagttag gtataaatca atcacctata atggctcaag
    19081 atataaaaat aaaatatcca tttgcttttg aactagctaa tatagctaaa aagacaatag
    19141 aaaaagattt gaagatagaa attaatgaag atgaagttgc aaacatagct ctacatatag
    19201 gaggagcagt tgaaagagca tcttataata ataaagataa ggttttcaaa actataatag
    19261 tttgtacttc aggcataggc acatcaatgt taataaaagc taaattagag aatatattta
    19321 aggaaaaatt agaaatacta aagattattc cttcatactt agttgaatat ataaaagtta
    19381 tagatgttga ttttgtaata tctactgttc ctattgaagt aggagatgtg ccagtaataa
    19441 atatatctcc aattttaagt gagaaagaaa ttagattaat tgaaaaatat atagaaacag
    19501 gaaatatata cctagattta gatataaaaa gcctgtttga tagtgaattg ttttttactg
    19561 atttggtgtt taaaaggaaa gaagaagtta tagattttat ggcaagtaaa cttgtacaaa
    19621 agggctatat agatgaggat atgaaaaaat cttactttga gagagaaaaa atagcaacaa
    19681 cagaaatagg gaatatggta actataccac atggagctat tggaaaaatt tttgataata
    19741 aaatagcaat tggtatatta aagaagccta tttcttggga aatatcagat gttcgtttaa
    19801 taataatgtt agcccttgat aaagataaaa tattagatta tgaagtaata ttttcaaaaa
    19861 tatataaaag agtagattct atagcaaaag ttataagtat atgtgaaaat agtagttttg
    19921 aaaaatttgt aaatttattc aattagatgg gtagtattgt atatacacct gtaaatttgg
    19981 ataatataaa actaaataag ataaaaacga taaatttctc ttttaattaa gagaaatttt
    20041 ttgactttca aggatattaa tataaagtta agatataaac ataaagcaaa taaataaaaa
    20101 agaggtggca atatgattta tacagtgaca ttaaatccat caatagatta tatagttaag
    20161 ctagatgagc taaagacagg aagtacaaat cgtgtaaaag aagaatacgt ttatccagga
    20221 ggaaaaggta taaatgtatc tcgcatattt aaggaacttg gaaatgataa tacatcttta
    20281 ggttttataa gtggttttac tggagaatat ataataagaa cattggaaga aaaagaattg
    20341 aagacagatt ttataaagat aaaaagtggt ttttcaagaa taaatgtaaa aataaaggcg
    20401 ttaaaggaaa ctgagattaa tggacaaggt ccaaatatag atgatgaaga cattgatact
    20461 ttatataaaa aacttgataa gctaaatcaa gatgatattt taatattggc aggaagtata
    20521 ccatctactt tagatgaaaa actatacgaa aatataatgg ctagattaga aaaaaagaat
    20581 ataaaagttg tagtagatgc aactaaaaat cttcttctta atgttttaaa atataaacct
    20641 tttctaatta agcctaataa cgacgaatta gaagaactat ttggtgttaa attaaattca
    20701 attgaagaca tggtaaaata tgctagaagg ttaaaggaaa tgggagctat aaatgtcttg
    20761 gtatcaatgg gaaaagatgg agcattgttg attacagagg aagatgaagt gcttataagt
    20821 gatgttccaa aaggaaaagt aaaaaattct gtaggagcag gtgattcaat ggtagcagga
    20881 tttatatctg ggtatttgaa tactgggaaa tatgattatg ctcttaaatt gggagcagct
    20941 tcaggtagtg caactgcttt ttcatatgac ttagccaaaa gagaatacat tgataaattg
    21001 gtaaatgaga tatctgtaaa gcagttttaa aatattaaaa aatatataga aaggtaatta
    21061 atatttatga aaattataga tttattagat gaaaagtcaa ttaagttaaa tttaaattca
    21121 aaaaccaaat cagaagcaat agaagaatta gtagatttgg tcgcaaatag tggaaattta
    21181 aatgataaag aaaactacaa aaaagcaatt ttggctcgtg aagaaatgag tacaacagga
    21241 attggagaag gtgtagctat acctcatgct aaaaattctt cagttactaa agcttgtata
    21301 gcagcagcag tgtcaaaaga aggtatagat tatgaatcgt ttgacggaag tttatcaaat
    21361 ctatttttta tgatagctgc accagacgga gctaacaata ctcatcttga agtattatca
    21421 agattatcta caatacttat ggatgaagac tttagaaata aacttataaa ttcaagctct
    21481 gaaaaagaat ttaaggaaat aatagataaa aaggaaagag aaaaattcag tgaagaatat
    21541 caagatgaga aagttactga aaaaaatata atagatacta aagagaatga tgctaataag
    21601 tatcctaagg ttttagcagt aacagcatgt ccaactggaa tagcacatac atttatggca
    21661 gctgaaagtt taaataaaat ggcagaaaac aaaggtgtat caataaaagt agaaactaat
    21721 ggttcagcag gtgttaaaaa taagcttaca aaagatgaaa tagaaaatgc aacatgtata
    21781 atagtggcgg cagataaaaa tgttgagatg gctagattta atggtaaaaa agtcataaaa
    21841 actaaggtgg ctgatggtat acacaaagcg gaagaactta tagacaaggc tgtaaatgga
    21901 gatgctccaa tttaccatgg aggagatgga agtcacaacg aatcaaatga agaaagtgaa
    21961 agtggattta gaaaagtata caaacactta atgaatggtg tatctaatat gttaccattt
    22021 gttattggtg gagggatact tatagcgata gcctttttac ttgatgatta tactattaac
    22081 ccttcaaact ttggttcaaa tacacctata gcagcatttt ttaaaggaat aggagataaa
    22141 gcttttggat ttatgttacc tgtacttgct ggatatatag catactctat atctgataga
    22201 ccagcgtttg ttgtaggatt tgtaggaggt gctctagcag gtgatggtgg ttcaggtttt
    22261 ttaggtgcac tacttgcagg ttttatagca ggttatttag tagaagggct taagaaaata
    22321 tttagtgtac taccagcttc attggaagga ataaaaccag tattgttata ccctttactt
    22381 ggaacacttt tgatgggaat tataatgaca tttttagtaa tacctccagt tacagcaatt
    22441 aataatgcaa tggttggatt tttaaatggt ttaggtggaa cttcaaaaat agttttagga
    22501 ttagtattag gtggaatgat ggctgtggat atgggtggac ctgtaaataa agcagcatat
    22561 gtatttggag ttgcatcact tgaatcaggg cagtttgaaa taatggcagc agtaatggct
    22621 ggtggtatgg ttccgccact tgctatagct cttgcaacta cattttttaa gaatagattt
    22681 actaaagaag aaagagattc tggtaaagtt aattatgtaa tgggattatc atttgttact
    22741 gaaggagcta taccttttgc agctggagac ccacttcatg ttataccagc ttgtgttgga
    22801 ggttcagcag tggcaggagc acttagtatg ttgttcaacg cagctcttcg tgcacctcat
    22861 ggaggagtat ttgttatacc agtggttacc catccatttg catatatatt agctatagct
    22921 gtaggtgctt tggttggaat gatgctttta gcattactta aaaaaccatt aaatcaagaa
    22981 gtatagcaat taaaggatat tcaagaactt aaaatatgat gttttataaa aattaatata
    23041 ttgctgaatt attttaaaat aaggatttta tgaggctgtt atataacgat ttgtaattgt
    23101 tatcttgaca gtcttttatt tttatataag taaaataagt gtgttttgct ttatttgtat
    23161 ttaaattgtt tttaagttag aatataaaga aaattatata ttattttaat at
//
LOCUS       DAMJVK010000063        22877 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.064, whole genome shotgun sequence.
ACCESSION   DAMJVK010000063 DAMJVK010000000
VERSION     DAMJVK010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22877
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.064"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            223..1659
                     /locus_tag="PC414_002469"
     CDS             223..1659
                     /locus_tag="PC414_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDF2464857.1"
                     /translation="MICTIKPLKISIKKSTEPLIEFISDTTYKNKSSSYIKEVYFTRY
                     IGVDDSIDKYIEKIKNIDTYFFNNPTIPYIRLNNLKINFDKEQTDKMLKIFNIYNSSK
                     FNSWDLYNIDFPCKIENDTLNWTKKIAFKNTLDLFSTSMPNCTSSIIKNFAVKLLCWI
                     DYFLPKLFYNKVKTTISPKIVYFGNIKRQELFFLYFLSQLGCDILYINPNKDILDLYP
                     ECSKFSTLFELSRKTPNILEIPFERHITNINYSENTNKTSPTTNKPNSKNTLNNTNLT
                     SKKIVQDEEVELSYEDLAKLSASVVMIVVCNNENKPFKSGSGVVINNEGYILTNLHVV
                     NDGYSFLVRFENDDKVYTSYQIIKYHSDYDLAVIKVDRKCKPIPVKVSKKPVRGQKIV
                     AIGSPLGLFNTVSDGIISAFRDFETVQMIQFTAPISSGSSGGALLDMFGNLLGLISAG
                     YDDGQNLNLAVESSLVKIFANNFIEIVN"
     gene            complement(1751..3625)
                     /gene="asnB"
                     /locus_tag="PC414_002470"
     CDS             complement(1751..3625)
                     /gene="asnB"
                     /locus_tag="PC414_002470"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HDF2464858.1"
                     /translation="MDGNISIYYKGDISKKEDIKFKIKESVNNTRLSSRIEYKDEEKT
                     FDFERFNVIDAIYGNKPFEKDGKVIVFNGNIYNYHEIKEELIKKGHNFTTNDDIEVLL
                     ASYIEFGKNCVHKIKGMFNFIIYDRENESIFGARDLFGIKPLYYINKENAIIFSSEYK
                     FLLEYMKNLNINERSLQSYFSFQYVLPEDTMIQGIRLIPAGHYFRVENGILSLKRYNK
                     LEFRSSTKFFYTKNHLGNRDVNEDDIRNIVVDSIVTHMEEEKEIGTFLSGGIDSSIIT
                     TVASQINPNIKSFSAGFSVKGYSELEVAKKTADKLGIENIQINITQDEYIKSLPNVIY
                     SLDDPIADPSEVGLYFLIKEAGKHVKVALSGEGADELFGGYNIYKEYSTMKSVVNSPT
                     YIKSILGRVSELMPNIKGRNYLYRATTPLEKRYIGNAKVFENSEVKRFFFKYKEKNIY
                     EYLLSNLYRDAQKNNYDYISKMQHIDVNTWLQGDILQKISKLSTAEQVELRVPFLYKD
                     VFDVAKNLRMEQKINKNNTKVLLREAFREIVPEHVVQRKKLGFPTPIRVWLKDSLGDI
                     VKETIYNSNVDEFIDKKYAIKLLDTHLKGHRDNSRKIWTIFTFCLWHQLFIEHKNVEY
                     "
     gene            3828..4502
                     /locus_tag="PC414_002471"
     CDS             3828..4502
                     /locus_tag="PC414_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HDF2464859.1"
                     /translation="MSLFIISQCITSFAFTPENNKFKVKPLPYAYDALEPYIDKETMK
                     LHHDKHYQAYVDKLNAALEKYPELYNYSLCELLQNLDSLPKDIATTVRNNAGGAYNHK
                     FFFDIMTPEKTIPSESLKEAIDRDFGSFEKFKQEFQKSALDVFGSGWAWLVATKDGKL
                     SIMTTPNQDSPVSKNLTPIIGLDVWEHAYYLKYQNRRNEYIDNWFNVVNWNGALENYK
                     NLKSQD"
     gene            4615..5151
                     /locus_tag="PC414_002472"
     CDS             4615..5151
                     /locus_tag="PC414_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDF2464860.1"
                     /translation="MIKETHERGGYIFALLILPFINNLYLVKYDIAYKIVLIIIYIYF
                     AYLGSLFPDIDMRGSYISKKFMLIYKLFGSRFRHRGFTHSLIALLLIYSFFKSLTIFT
                     NNNIVFSCLSSGFIIGYLSHLCLDLITKEGIELFYPITINISLLPIKTNSKTEKFISK
                     LLNFIVIFLIGYQFYILL"
     gene            complement(5317..5937)
                     /locus_tag="PC414_002473"
     CDS             complement(5317..5937)
                     /locus_tag="PC414_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDF2464861.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
     gene            complement(6251..8389)
                     /gene="vanT"
                     /locus_tag="PC414_002474"
     CDS             complement(6251..8389)
                     /gene="vanT"
                     /locus_tag="PC414_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HDF2464862.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKRLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
     gene            complement(8407..9213)
                     /locus_tag="PC414_002475"
     CDS             complement(8407..9213)
                     /locus_tag="PC414_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDF2464863.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            complement(9210..10310)
                     /gene="vanG"
                     /locus_tag="PC414_002476"
     CDS             complement(9210..10310)
                     /gene="vanG"
                     /locus_tag="PC414_002476"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HDF2464864.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNDDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            complement(10571..11653)
                     /locus_tag="PC414_002477"
     CDS             complement(10571..11653)
                     /locus_tag="PC414_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2464865.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFINHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIIIFKVIIPIVGNL"
     gene            complement(11703..12404)
                     /locus_tag="PC414_002478"
     CDS             complement(11703..12404)
                     /locus_tag="PC414_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanG-Cd-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HDF2464866.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KNLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            complement(12838..15369)
                     /locus_tag="PC414_002479"
     CDS             complement(12838..15369)
                     /locus_tag="PC414_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDF2464867.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDENEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVNEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSNYKVNLFDVIEHKQEDILAKIAVSQGVLFGTPTILGDALKPIWDILISL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLYSY
                     TGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVV
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDGKAQKVVFKDGDSVDC
                     DMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTNVKDIYACGDVAQVGNISLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            complement(15477..16094)
                     /locus_tag="PC414_002480"
     CDS             complement(15477..16094)
                     /locus_tag="PC414_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDF2464868.1"
                     /translation="MCKKTYLLVVSMLIAGLLTACSGSPTKDSGQAQDNTKKEANASN
                     NALKDEKNNENLMEQDFKVPYTDAINIFKDKYKDADIVDLSLERDLNKFVYTVEGVDD
                     NNEYKMKIDANTKDVLEDKTEKLDSEDLNGVARKEKLDLNDIMTPQQAMEIALKEQNG
                     IVKEWSLDKDLDVTFYKIRIDKDKNEYDIKVDSKKGTVLKVEKED"
     gene            complement(16456..17340)
                     /locus_tag="PC414_002481"
     CDS             complement(16456..17340)
                     /locus_tag="PC414_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HDF2464869.1"
                     /translation="MDTTKIDDISKLDLNNLDINKMDMGGIMESLLEWATTSGVKLII
                     GILILSIGFKIIKKFVNHVMLLLSKRDIDLTLRKFLKSLLLSVLKVAVIIIVLEYWGM
                     SLSSFAAVIASAGVAIGLALQGSLSNFAGGFIILLIRPFKVGDYVEAAGHGGTVEQIG
                     LFYTQLVTPDNKQILIPNGSVSNDSLINYSAKNTRRVDLTFSVGYEDDILHVRRVLKD
                     IVNNHKLIINEPEPFIGVVEHGDNAIKFATRVWCKTEDYWTIYLDLLEEVKVRFDEEG
                     ITIPYPKMDLTVKELNKI"
     gene            complement(17531..18781)
                     /locus_tag="PC414_002482"
     CDS             complement(17531..18781)
                     /locus_tag="PC414_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2464870.1"
                     /translation="MKSYTKAKWSVWGIITFSFVLVLFLRMSTAVVSDNLANELGFNS
                     IQISNIASFCLYAYAFMQIPAGILIDKYGARKISSLGIIMASLGSILFGLIQSIELAY
                     ISRVIVGAGTSVILLCILKIQGRWFNKSEFASATAKFSFVGNLGGVLATFPLVFLSEL
                     VGWRNSFLLIGIIGVVIGCFMYIIVRDTPKEYGFNVDTEPYEKSEKVNIVDGIKSVIK
                     NKSTWYNSMIMFSFVGLTSAFISLWGVRYIMDVYGVSKSFSAFIVSFFTYGFIFGSII
                     MDFVFAKIRSSKFNIIKFGAMIDLFIWIVIVVVYQVKPPIIVLPISFFIMGCIVMSHL
                     QVFNDAKYKNKEIYSGLATSVINTFEFIGSGIINLIIAISLQVNSYNIVDGYKKGFVV
                     FIVLSIITIVSSHIGVKNDDFKAI"
     gene            complement(19003..19767)
                     /locus_tag="PC414_002483"
     CDS             complement(19003..19767)
                     /locus_tag="PC414_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2464871.1"
                     /translation="MLGKLLKYELKASGRIFIPLYIAILIVAVFNGIFMNTNILQVQG
                     IGILVLTSLFMALGVLTIVVTIQRFRKNLLGDEGYLMFTLPVSTSSLILSKCITALIY
                     AVLSFIVAVFTFGVLMLFGTSGILLPEILDLFNTSFKWISENFLDILLLVVVMFISYS
                     SFILLLYTSISMGQLPKFNKHRNIVAFASFIAINIVISIVGDAVGSILPNEDTNMVYH
                     LYQSPSFMLAILGSLVVAIALFFATKFILDKKLNLE"
     gene            complement(19761..20486)
                     /locus_tag="PC414_002484"
     CDS             complement(19761..20486)
                     /locus_tag="PC414_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2464872.1"
                     /translation="MDKNISNVSQDGIVEFKGVNKSYGTKNVLKNIDLNIPKGKIVGL
                     LGPNGSGKSTMIKLMNGLLQPDNGEIMINGMKPSIDTKKIVSYLPERTYLNDWMKVSD
                     LLKFFYDFYSDFDVRRANEMIKSLDIDVNEKLKTMSKGTKEKVQLILVMSRNASIYIL
                     DEPIGGVDPAARSYILKTILKNYSEDSTLLIATHLISEIENICDEIIFISKGEIVLQG
                     DVEAIREEKGKSIDALFREEFKC"
     gene            complement(20488..20856)
                     /locus_tag="PC414_002485"
     CDS             complement(20488..20856)
                     /locus_tag="PC414_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2464873.1"
                     /translation="MEWELDNNKPIYIQLVEHLKLKIISGEIKIGSKLETVRALAEDA
                     EVNPNTMQKALTELERQGLVYSQRTKGRFVTDDKEKIKAMKEEIANVEINTLKVTLEK
                     LGYDRDEMLKLITENLKGEL"
     gene            complement(21032..22576)
                     /locus_tag="PC414_002486"
     CDS             complement(21032..22576)
                     /locus_tag="PC414_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HDF2464874.1"
                     /translation="MRNLQKKLMFFLFICIILQITYTTPVYSLCKLYKNNSINNFESI
                     VSNNQLISKHAIQIILIMAIICVILIFYIIYDKLNFKIKLQRIAYTDNLTGANTIDKF
                     VIDANKILCKNTQVKYALLYIDIDKFKYINDLFGYEVGNEILRNLTKIIKSNIFEEEM
                     FARISADNFIIIMKYIEEEDITKRLKTIFEELDLFNNNQEEKYKLVLSCGIYFILPED
                     RDINSIIDRANIPHKMAKGGHKSSYAFYDNKIHDQEIKEKEMENTMFSSLENKEFIIY
                     LQPKIELNTGEIQGSEALVRWKRPDKGLIPPNEFIPFFERNGFVINLDLYVLEEVCIY
                     LRKWIDAGINPVTVSVNVSRIHLYCNNFIETYKNIIDKYNIPAKYIELELTESIIFDN
                     FDILIDIMNNLKKIGFLISMDDFGSGYSSLNMLKEIPMDILKLDQKFIMETYNSKRSK
                     IIVTKVIEMAKELGMKVISEGVETEEQFKLLKEVKCDMAQGYLFGKPMPIEEFEHLIV
                     SNLVRG"
BASE COUNT         7885 a         3838 c         2389 g         8765 t
ORIGIN      
        1 tagcggtatt agattacaag cattattcta acttttttta tattaagacc ttacctttca
       61 tccctacacc taaaggagag ggcttttcgg caattttatt gtaattatat atcatttata
      121 taaacaagta catattttat tttgaaacta caaaattatt gtactatatt gttttaagtt
      181 gttttataat agattatatc atcttattat aggagaaaaa atatgatttg tactattaaa
      241 ccattaaaga tttcaataaa aaagtctact gaacctctaa ttgaatttat atctgatact
      301 acctataaaa ataagagttc ttcatatatt aaagaagtct actttacaag atatataggt
      361 gttgatgatt caattgataa atacatagaa aaaattaaaa atatagatac ttattttttt
      421 aataatccta caattcccta tattagatta aacaatctaa aaataaattt tgacaaagaa
      481 caaacagata agatgcttaa aatttttaat atttataata gttctaagtt taacagttgg
      541 gatttatata atatcgactt tccatgtaaa atagaaaatg acaccttaaa ttggactaaa
      601 aagattgcat ttaaaaatac tttagattta ttttccacaa gtatgccaaa ttgtacatcc
      661 tctatcatca aaaactttgc agttaaactt ttgtgttgga tagactattt tctacctaaa
      721 ttattttata acaaggtgaa gactactatt tcaccaaaga tagtatactt tggaaatatt
      781 aaaaggcaag aacttttttt cttatatttt ttatcacaac ttgggtgtga tattttatat
      841 ataaatccaa ataaagatat tcttgattta tatcctgaat gttcaaaatt ttctactcta
      901 tttgaacttt ctaggaagac tccaaatata ttagaaatac cttttgaaag gcatattaca
      961 aatataaatt acagtgaaaa taccaataag acttctccta ctacgaataa acctaactct
     1021 aaaaatacac ttaataacac aaatctaaca tctaaaaaaa ttgtacaaga tgaggaagta
     1081 gaactttctt atgaagacct tgcaaagtta tcagcttctg ttgttatgat tgttgtgtgc
     1141 aataacgaaa ataaaccgtt taagagcggt tctggagtag taataaataa tgaaggttat
     1201 atacttacga atcttcatgt agtcaatgat gggtattcat ttttagttag atttgaaaat
     1261 gatgataaag tttatacatc ttaccaaatt ataaaatacc attcagatta tgatttagct
     1321 gtgataaagg ttgatagaaa atgcaagcca atacctgtta aagtcagtaa aaaacctgtg
     1381 agaggtcaaa aaatagttgc aattggaagc cctctaggtc tttttaatac agtttcagat
     1441 ggaattattt cagcttttcg tgattttgaa acggttcaaa tgatacaatt tacagctcca
     1501 atctctagtg gtagttcagg aggagctctt ttagatatgt ttggaaactt actaggattg
     1561 atttccgctg gatatgatga tggacaaaac ttaaatttag ctgttgaaag tagtcttgtc
     1621 aaaatatttg ccaataattt tattgagata gtaaactaat caatcaaaat tctacttatc
     1681 ctattccata ataatcaaga gggtgaaaac attgcatgct tttgccctct gattaatatt
     1741 gaaaaaagta ttaatactcg acatttttat gctcaataaa taattgatgc catagacaaa
     1801 atgtaaatat agtccatatt tttctcgaat tatctctatg acctttaaga tgcgtatcta
     1861 aaagttttat tgcatatttt ttatctataa attcatctac atttgaatta taaatagttt
     1921 cctttacaat atcccctaga gaatctttta accaaactct tattggagtt ggaaatccta
     1981 attttttcct ttgaacaaca tgttctggga ctatttctct aaacgcctct ctaagtaaaa
     2041 cttttgtatt attcttattt attttttgct ccattcttaa attttttgcg acatcaaata
     2101 catctttata taaaaatggt actctaagtt caacctgctc agctgtagaa agtttgctaa
     2161 ttttttgaag tatatcgcct tgaagccatg tattcacatc tatatgttgc attttactta
     2221 tataatcgta attatttttt tgtgcatctc tatacaaatt agacaacagg tattcatata
     2281 tattcttttc cttatactta aaaaagaatc tttttacttc gctgttttca aaaacttttg
     2341 catttcctat atatcttttt tctaatggtg ttgtagctct ataaagataa tttcttcctt
     2401 ttatgtttgg cataagttct gaaactcttc ctaatatact tttaatataa gtaggtgaat
     2461 tgacaactga tttcatagta ctatactctt tatatatatt atatcctcca aataattcat
     2521 ctgctccttc tccagacaat gctactttaa catgtttacc tgcctcttta attaagaaat
     2581 aaagacctac ttctgaaggg tctgctatag ggtcatccaa agaataaatt acattcggta
     2641 atgattttat atattcatct tgtgttatat taatctggat attttctata cctaacttat
     2701 cagctgtttt ctttgctact tctaattcac tatatccttt gacactaaag cctgcagaaa
     2761 aagattttat atttggatta atttgtgaag ccacagtagt tataatagat gaatctattc
     2821 caccagacaa aaatgttcca atttctttct cctcttccat atgagttact attgaatcta
     2881 ctactatatt tcttatatca tcctcattca catctctatt gcctaagtga tttttagtat
     2941 aaaaaaattt tgtactactt ctaaactcaa gtttattata tctctttaaa gatagtatac
     3001 cattttctac cctaaaatag tgacctgctg gtatcagcct tattccttgt atcatagtat
     3061 cttcaggaag tacatattga aatgaaaaat aactttgtaa gcttctttca tttatattta
     3121 aatttttcat atattcaagt aaaaacttat actctgagga aaaaattatt gcattttcct
     3181 tatttatata ataaagtggt tttatcccaa ataaatctct tgctccaaat atactttcat
     3241 tttctctatc atagattata aagttgaaca ttcctttaat cttatgtaca caattcttac
     3301 caaattctat ataagatgct aataagactt caatatcatc atttgtagta aaattgtgtc
     3361 cttttttaat taattcttct tttatttcat gatagttata tatatttcca ttaaaaacaa
     3421 taacttttcc atctttttca aatggtttat ttccataaat agcatcaatt acattaaatc
     3481 tttcaaaatc aaaagttttt tcctcatcct tatactctat tctactagaa agtcttgtat
     3541 tatttacaga ttcttttatt ttaaatttaa tatcttcttt tttagatata tcacctttat
     3601 aataaattga aatattgccg tccatataat caaaattcct ttctgtcctt tagtcttatt
     3661 ctatatttca tagttttgtc tttggcttta aaacttattc aatttaaaat gaaattgcta
     3721 ttaaattctt ataaattatc tgttaatatt tcatattttt tttatttcta gaaatactat
     3781 attaggaggt gatttttatg aagaaaaaaa tattaatacc agttattatg tctttattta
     3841 taatctcaca gtgcataact tcatttgctt ttacacctga aaataacaaa tttaaggtta
     3901 aaccattacc ttatgcatat gatgcacttg aaccttatat agataaagaa acaatgaaac
     3961 tgcatcatga taagcattat caagcttatg ttgataaatt aaatgctgct cttgaaaaat
     4021 atcctgagct ttataattat tctttatgtg aattattgca aaatttagat tctttaccta
     4081 aagatattgc tacaactgta agaaataatg caggtggagc ttataatcat aaattctttt
     4141 ttgatataat gacgccagaa aaaaccatac cttctgaatc tttaaaagaa gctattgata
     4201 gagactttgg ttcttttgaa aaatttaagc aagagttcca aaaatctgct ttagatgtct
     4261 ttggttctgg ttgggcttgg cttgtagcta ctaaagatgg caaattatct attatgacta
     4321 ctccaaatca ggatagccct gtaagtaaaa acctaactcc tataatagga cttgatgttt
     4381 gggagcatgc ttactattta aaatatcaaa atagaagaaa tgaatacatt gacaactggt
     4441 ttaatgtagt aaattggaat ggtgctttag aaaattacaa aaatttaaaa tctcaagatt
     4501 aattaaatat taaaatcctt ttgaaaaaaa cttatgtttg ctatataata taataaaaga
     4561 tagtctaaat ttaccaatat aatgtttata gtgacgattc aggaggattt ttatatgata
     4621 aaagaaacac atgaaagggg aggttatata ttcgcacttt tgatactccc ctttataaac
     4681 aatctatacc tagttaaata cgatattgca tataaaatag tattaattat aatttatatt
     4741 tattttgcat atttaggttc tttatttcca gatatagata tgagaggttc ttacataagt
     4801 aaaaaattta tgttaatata caaattattt ggaagtaggt ttagacatag agggtttact
     4861 cacagtctaa ttgccttatt acttatttat agttttttta aatctctgac aatcttcact
     4921 aacaataata tagttttctc atgtttgtcc agtgggttta taataggata tctatctcac
     4981 ttgtgtcttg accttattac taaagaaggt attgaattgt tttatcccat taccataaac
     5041 atttctttac tacctataaa aactaactca aaaacagaaa aattcatatc taagctatta
     5101 aatttcatag ttattttttt gattggatat caattttata ttttattata gttgtagaaa
     5161 aaataaataa caatattttg aactaagtat aaataagttt tatatatttt agtttacaaa
     5221 agaactaaaa attttgtacc ttaatacaaa aaataaaacg tatcttaaaa taatttagta
     5281 ctaaatcatt ttaagatacg ttttatttcg tacaagctaa atcattttat catctttatc
     5341 attgtgatgt tttttatgat ggaaatcata attatgattt tcaagatttt tatttactct
     5401 atttccaatc ttccatgaca aaaacaccaa taatataaat gatgctatct ttaaaggact
     5461 tgttataaaa ccttctatat cacttgccac gtaacttata actataaaca ttacaaattt
     5521 tcctgccaac attgctggca caaaatcttt taaatctttt ttaccaaaag ctgatgcgat
     5581 agtaactaaa caaccgggca taaatggaca ggcatacgct ataaataata acttaaatcc
     5641 ctgtttatcc atccaactta ttgctttttc agttttacta tttctcaact tattaaaaag
     5701 cttactatca ttaaatctgc ttattaataa gaataatgaa gttgtcccaa gtcctgagcc
     5761 aatccatgat aacaaacatc caataaataa tccttgtatt gcagcatttg ctgtaactat
     5821 agcaacaagt ggtaaggctg gtataaagct ttctataaat gcacttaata agccgattat
     5881 tgtggctaac acccaataat tttgagtcaa atcaagtagt tcttgtatat actgcatatt
     5941 gcttttcccc ttttttaact ttaactatta ttatatactc tatatgttta ttatatacca
     6001 atctcaaaaa atgaatctaa tattaaaaag taaataatct atataagttt acagttaagg
     6061 tataattatt ttattatata ttataacaaa aaaggtgatg tttcaaaata aaaatctgaa
     6121 acatcacctt tttataactt aaattgtcat gtaagttata aaaaatatat attagacata
     6181 atattactaa aaatgtctag tcagagtaaa ataaattaca cagtacaaca aatattacta
     6241 tttaattgag ttacttcttt ataacattta accttcttcc tactctgctc aataattcat
     6301 tcgatatact acatgagtta tttgcaactt cagaagcaga taattcagat aaattatctc
     6361 ttcctattat tattgccgta ttaccaactt ctacattagg tatatctgta atatcaacag
     6421 caagttggtc catgcaaatt cgacccacta ttgctgctct acaaccattt attattacgt
     6481 aactttttcc acaggataaa tttctaggta taccatcggc atagccaata gataatatag
     6541 caattacgct atctctgctt gcaataaaga ctcttccata tccaaaacta tcatcttttt
     6601 gtatttttct aataagaatt acttgtgatt ttaaggataa aacaggtcgt aaatcaagtt
     6661 gcaatttagt cgtatcattt ggagaactta atatcccgta taatgcaatt ccaattctta
     6721 cataattaca cttaagattg ggataattaa aaaaaccata actactttga atatgtactt
     6781 taggaatttt tatacctttt tttaacagaa aatcaattaa cttataaaaa cattcaattt
     6841 gtttatttgt aaagtctaca tcttcatctt tcagactatc tgaaacacac aagtgtgtaa
     6901 atatgccaca tatatctaag tattttaatt taaatgcctt taaaattgac ctcacatcat
     6961 taacatcaaa acccagtctg tgcatacctg tatcaatttt aatgtgtgat tttatgttgt
     7021 atttctgttt gtttaaagag atagcatgat taaggtcaat aacactctgt gtaagattgt
     7081 atttatgaag ttcctttgcc cttattgggt tagtatatcc aagaatcaat atttctccta
     7141 gtattccata tcgacgtaaa tcaatcccct catctattgt tgctactgca aatgctttta
     7201 cacctatttg gtttgcacaa gtcgctactt caaaagcact atgaccatag gcattagctt
     7261 ttacaactgc catcaattca caatcttctg gcatagcttt ttgtatcatc tttacattat
     7321 gttccaaatt atttaaatca atttcaatcc aagctctatt ggtatttacc ttatgttctc
     7381 ctttcaagtc tttttttaga ggtttaagaa tcaatataat aattatagaa gctataaaag
     7441 atgctatact taccactata taatgaataa ggctattgtc aaccaagaca gtttgtattc
     7501 cgaatatttt agaaaacata cgaataacaa caatcatcat aggatgtata atataaataa
     7561 ttaatgatga tgttcgcaat aacctagctc gagaaccttt caaaaatgtt aaggctgtaa
     7621 ataagaaata catacatggc aataacataa tatacatgct atcatgacgc tgtaccccta
     7681 gtttatgtaa aaacattccc tcacataaca taagtaagaa tgatataaga aatccaatca
     7741 agctattttt agttgacata taattagatt tattagatat tattccacca agtatgaaaa
     7801 atacaggtgc aaaaaatata ccatttcgtg tataatctga tacttcaaaa atatacccat
     7861 ataaattctt taatagtggt attttttcta aaaaaccata ataactatca ccaaacatac
     7921 caattacata aataacaata gtgattacta gtgagtactt taaacctagt ctttttacaa
     7981 gaaagtatgc aatacttgca ccaataattg atgcaggtag ataccataaa tgatataaag
     8041 tcccatcaaa tacaatgtcc ttaataatat tgggcactaa attattcatg gaaaaatatc
     8101 cagtgtaaat atttaaggga atataaatta caatagctat cccgtaaata aataatacat
     8161 tttttagaaa agatttcagt ttattacaat tataattata tgttgaaatt agaaaaaagc
     8221 ctgatagcat aaaaaagaat ggcacagcaa tacgagcaat tatactagtt aacacaaagt
     8281 cagccatttt actatatgat aataatggag atgtatgaat tgcaattata agtaatgcag
     8341 ctataatcct aaaataatca agccctgcat aagatttatt cctaatcatt cttgttcctc
     8401 catttcttag tcctttctat ttctccaaaa tgtcacaata aaaccatcct tattgttacc
     8461 cgatatttga tatacacatt gttcaggtat agagattctc acttcttctt tagtatgaat
     8521 aggcacataa aagatttcta taattttttc tttatcttct atttttaaac aatttgtata
     8581 cgaaaatctc ttaatatatt ctatatattc ttcaagagcg agattcattt tattgataat
     8641 ctcagaatgc ggatatccca cataacgaaa atgccatggt tcttgagata ttcctgttat
     8701 ttcttctttt ttacactgat aacgctctat aaaaccataa tgtagtgctg ccttacgaaa
     8761 ctcattacaa ataccattat aaggaaattc agggcagata aaatctataa cttctttctt
     8821 taaccctaaa tcaatagcaa gccctgtctg atgttcactg tgatttggaa gtgctacaaa
     8881 ctttttagta aagtcttttc cattgtcacg cagagaactt gaatatatat cttgttgttc
     8941 ctctgcagta cgatatccac ttacaggtac tatttcattt attccacata tactttttaa
     9001 aatattttgt aaaatgtttg tagcttcacg tttcataaga atacttggaa accttgtatc
     9061 tacaggaatt aaatctatat cttcagatgt tttaactggt aaaaaagcat ttactagaat
     9121 tagattccca tcataaactt tatctttagt caataagata gtatttgtca ttttatcttc
     9181 tttattttta tctaataaaa tattattcat cattttatgt acacctcaat tatcttgtcc
     9241 aatatttctt caaaagacaa tccaatacct ttcatcatat ttggataacg actatgagaa
     9301 gtaaagccag gaattgtatt gacttcatta aaaataattt caccagatgg agttaaaaac
     9361 atatctactc tagcaaatcc agaacatcca agagttctat aaataatttt tgctgtctct
     9421 tgtattcttt tctcacaatc caaatcaatt ctagctggca tatgaatttt tgaacttttc
     9481 aatgtatatt tttctgtata atcaaaaaat ccttttgaca attctatttc atctactctt
     9541 ccaacaagta agtcatcgtt tcctaaaact gcacacccta cctcaaaacc atcaatattt
     9601 tcctcaatta ttacttcatt atcatgttca aaagccaagt ctactgctac ttgtaactct
     9661 tcttttttat aaattttact aataccaaat gatgaacctg cacgcacagg ttttataaat
     9721 aatggataag ttaagtcttt tgacagttca gccacattaa atttatctga gtattttagt
     9781 aatattgatt ttggtacttt aataccttct gaatgaacaa gtttatgtga tttgtctttg
     9841 tccatacata atgctgaaga aagagtacca catcctataa cctgtatacc tgccaattca
     9901 aataacccct gcactgttcc atcttctcca ttttttccat gcagtacagg aaacacaaaa
     9961 tcaagtttag tgtatttaat atcattttca catgattcaa tagcactatt tggaatatga
    10021 ttctgagaaa cagcatcagg aattaaaaat tcaataatac catttttgac acggttctgc
    10081 gaaacaataa ctggaattat atcagtatcc ctttcaaacc aagtgttatt ctggatatta
    10141 ttataatctc caatataatg ataccaatta ccctcccttg taattccaat tggtataata
    10201 tcatattttt ccgtattcaa attctcaagt actgcaaaag ctgattgtaa tgatacatca
    10261 tattctgttg agtttccacc aaatataact gcaatttttt tattattcat ctttgattct
    10321 cctttgttga tttatttatt tttatacaat ctacagaaac atgttaaaat actttttcta
    10381 tttcaaatcc aatcaacctt cacttacaat tgcatctgtt ccacataata tattttcaca
    10441 tttttctatt gatataatta ctgtatcaaa ataaaagcac tcttgttttg atacagtaat
    10501 tatatcaata acaagagtgc tctttattta tttatcctaa agaatttcct atttttttat
    10561 acttaaattc ttacaaattt cctacaatcg gaataataac cttaaagata attatattgt
    10621 tctcgctatg agctgttatt gtcccgttat gaagctccac tatttcttta gctatagcaa
    10681 gtcccaaccc agctctacca ctgtttgaat tacgagatgt atccaacctg taaaattgtt
    10741 caaaaatacg ctcaagtttt tcctctggaa tcgtattgct atgatttata aatttaagct
    10801 ttatataatc atcctcctgt acagctgtta tttcaatagt agtatcttca aaactataat
    10861 aaacagcatt tcgtaataag ttatcaaata cacgttgcat tttattaaca tcacacctta
    10921 tcatcatatt tggtgtaaca ttcagtgtat attttaaatt cttactcatc aaagtaggtt
    10981 taaactcata tgttagttgt tcaagcatac gagttaagct tactgtactg tattcaagtg
    11041 atatatttga taaattaaac cttgtaattt caaagaattc attaattaaa tcttcaagac
    11101 gctctgcctt atccaacgaa atagataaat atttttttcg cagttcttca gaaatctgac
    11161 tttcatctcg aagtagtgtc agatacccta taactgatgt aagtggtgtt tttaaatcat
    11221 gtgcaagata aacaataagt tcattcttac gttgttctgc catttgtgtc tcaaatttac
    11281 gttgttcaag tatatgttta attgagttta ttttcttttc tattgccaat aactctggag
    11341 ataaagccac ttctccaaca tcttctttga taagagagtc aataccttga tttatttcac
    11401 taaaatattt tgtaaaccaa tttaaataaa cccggaatat tataaaaaac acaatcacca
    11461 tagctagtat catgaagagc tccatgctat tacggaaaat ctgctgatat aaattcaatg
    11521 cagctttata ttccatacga aaagcatttt caaagaatga tactatccag atagaaaact
    11581 taccatacaa tataaaagaa tataaaatcc atatgaaaat aatggctata aatgccatta
    11641 aagctgttct cataaaaatt ttatttctca cttgagtaaa gtcaggattg actttaatat
    11701 ttttactttt caattgtata acctactccc catacagttt ttataaattt cgggttttta
    11761 gaaggttcat gaattttttc acgtaatcgc cctatatgag ccataactgt gttattatta
    11821 tcaagatatt tttctcccca tacagcttca aacaattctt cagaaggaac cacttttcct
    11881 tgatgttcac ataaatacca aagaatagaa aattcaatcg gtgttaatgc tacttctttc
    11941 ccaaataaac tacatttatg tgtttcttta ttgataatca atcctttaat atcatactca
    12001 ttaacaatta tcgactgttg ttcataagaa ttattatatc gcatatatct tcttaattgt
    12061 gtctttactc ttgcaacaac ttccaaaggg ttaaatggct ttgttatata atcatcagca
    12121 cctatggtta aacccattat cttgtctata tcttcaattt tagctgttaa cataatgata
    12181 ggataatagt atttttctcg aattttctgg caaatatgaa atccatcaat atcaggaagc
    12241 ataatatcta aaatagccat atccaaattt ttagattcaa tacattctag agcttcctta
    12301 cctgtataaa acttgtatac tgtaaaacct tcatttttta aatatacttc aattaaatct
    12361 gctatttctt tttcatcatc cacaatcaaa atattttcgt tcatagatta tttttcactc
    12421 tccttattat cgaggtcatc atttataata ttggtaaatt tacatatcaa ataattacaa
    12481 gcatatttgt aactaatttt tataagaaaa gaactactac atcagaatgt gttgatgcta
    12541 ctattggaac acaaaaactg agattaattg ttattataaa aactccccta acacgtcctt
    12601 attatcgcat aacaggtatt aactttcaat aaaaatatct atcagtcaaa cacaattcaa
    12661 tttattatac cagagacaat ccatataaaa aacaattaaa cctatgtatg taaccacaga
    12721 cattactaaa atttccatgt tattttatat aaaaattcaa aaaaggagtt gaatttaaaa
    12781 tttgattaga aaaaaatcaa ttttgagaca actccttttt attttaatgt atttaaatta
    12841 tagtatatca gccttcataa tatcctcaaa atcacctttt tcagatatta atcttatcaa
    12901 tttaacagta cagcttaaat catttataag tatagctcca actaatgaac catctttcat
    12961 aaataatttt ttatatattc tttgaccttc atcttttgat gaaatttctt tatcaaaatt
    13021 ttgtatatta ccaatactaa atacttttac attcattgca tctaatgaaa ctggatatac
    13081 ttcactttca aatgtcaaat tatctccact tgcatttgcc cctgctattt ttcccatttc
    13141 tactgaagat ggccatattg ctaaactaat atttcccact tgtgcaacat ctccacaagc
    13201 atatatatct ttaacatttg tctgtaatgt cttatctaca acaatacctc tatcaatttt
    13261 tacagatgta tcctgaacca tttgagtgtt tgctcttaca ccaattgaaa atactaccat
    13321 atcacagtcg acactatctc catctttaaa tacaactttt tgagcttttc catcaccttc
    13381 aataccatca actgctacac ccaatcttac atctattcct gtatctctta cacattgttc
    13441 taatatttta ctaccttctt tatcaagttg ttttgatagt atgctatcca tggcttcaac
    13501 tactactact tctaatcctt ttagtctaaa ttcccatgca gcttcaagtc ccaataaacc
    13561 gccaccaatc actacaactt tcttagattt ttctagtgct tcttttacac tatataaatc
    13621 ttttatattt ctaagagtaa acacattttc taaatcatgg cccttaattg gtggtataaa
    13681 gtttctactt cctgtagcta ttacaagctt atcaaattta atagctccat cattcactat
    13741 aatagtttta ttcacttcat ctaatttatc tacattcgtt cctaatatta ctactatatt
    13801 atttttatca taccaatctt tatcttccat gtagaaatct gaaccaagtt cttcatttat
    13861 accatcagaa agtgcaggtc tatagtaagt caactcatct tcattagaaa tcatagttat
    13921 cttacaagtt ttatttcttt ttcttatagc atctgctgca taaaaaccag ctgctccatt
    13981 tcctactatt acgaaatatt catcagtatc ttttctaaaa gtaacttcat cttcttcaac
    14041 ttctataaat tggtcttcct tagctccaca tacagggcat acactaggag cactatcacc
    14101 ttcaaaaact tcattacaaa ttacacattt aaatttctta gttttctttt tacttccaaa
    14161 agtagaattt aacttatcta ggaatttacc tgtataagaa tataatttat caatctcttc
    14221 atttgatggt ttaaagttaa cagcaaaagg ttttactgct gtcattctaa gctgacttat
    14281 tctctccata gcattttcta taccttctcc actccatcca tatgaaccaa atactgaagc
    14341 aacttttcca ccatgaagaa ctgggtttaa cgatattaat atatcccata taggctttaa
    14401 cgcatctcct aatattgttg gtgttccaaa taatacacct tgtgatactg ctatttttgc
    14461 caaaatatct tcttgcttat gctctattac atcaaataaa tttactttat agttgctatt
    14521 tttttcaata tatgctttta tagcttctgc cataatcttt gtatatccat gtgcagaaac
    14581 ataacaaatt gtaacttctt tttctaactt tatttgttca tttacactcc agttgtaata
    14641 aaggtctata atttttctag gattttcagt taatactgga ccatgacctg gacatacagt
    14701 atctatatct aaatctttta tcttttctat agcagttacc atagatggtt taaatggacc
    14761 cattatacaa tcataatagt atcttaaagc atccaagtaa tccttttctt cattttcatc
    14821 aagtgtattt actatcttat cattactata gtgactacca aatgaatcac atgttacaag
    14881 agttttatcc tcttttatat atgtgtatat ggtatctggc caatgaagca taggtacaga
    14941 gaaaaattct aaagttttgt ccccaattga taatgtatct ccatctgtaa ctgcaacata
    15001 ttcaaagtct ttgtttacga tttccttaag atatttaatt gctgtttctg aagctactac
    15061 ttttgcattt ttagctaaat ccaaaagctt ttcaacgctt cctgcatggt ctggttcagt
    15121 atgacttaca acaatatagt ctattttttc aaaatctata tttaaatcat ttaatctttc
    15181 aatataattg tcaaaatgct tatctttaac tgtctcaaat aaaactgttt tttctgttcc
    15241 ttttaataca tatgaattat aagtagttcc ataaggtgta tacataatta tgtcaaatac
    15301 tcttaaatca gggtcaagcg aacctaccca atataaattt tcttttattt ttagtgattt
    15361 caatgacata ccgtactcct cctaaaatag ttatttagtc tctttatacc ctgcttgtta
    15421 tattttaaac aaaatttatt caaaaaagaa attattttaa agatagttta aatcttctaa
    15481 tcttcttttt ctaccttcaa tacagttccc tttttagaat ctacttttat gtcatattca
    15541 tttttatctt tatctattct tattttataa aaagtaacat ctaaatcttt gtctaaactc
    15601 cattctttaa ctattccatt ttgttctttt aatgcaattt ccatagcttg ttgaggtgtc
    15661 attatgtcat ttaaatctaa tttttctttt ctagcaactc catttaaatc ttctgaatcc
    15721 aacttttctg ttttatcttc taatacatct tttgtattag catcaatttt catcttatat
    15781 tcattattat catctacacc ttccacagta taaacaaatt tatttaaatc tctctcaaga
    15841 cttaaatcta ctatatcagc atccttatat ttatctttaa atatgtttat tgcatctgtg
    15901 tatggcactt taaaatcttg ttccattaaa ttttcattat ttttttcatc ttttaaagcg
    15961 ttatttgatg catttgcctc cttttttgta ttatcttgtg cttgtcctga atccttagtt
    16021 ggactcccac tacatgcagt taataaaccc gctataagca tacttaccac taataaatac
    16081 gtttttttac acataaaaac cctccttaaa tatattaatt tattctatat tatgccctct
    16141 aaacccttta tttacccttt tatattaaaa attcctccta ttttatcaat tttaaaactt
    16201 tatatttact aaaattaaag ttacataaaa attatactgt ctcaaaatgg aaacaagcct
    16261 ctttttcatt gttgaaataa taaaaatata ttttatttcg tacaaaaaga gtgcctcatg
    16321 aactaaaaag ttcattttga gacactccca tttttttata tttttactta caaaataaca
    16381 gtacttatta attttttaca aaccttataa gtatatctat atgatattct taaatttaaa
    16441 cactatatta aaaaattaaa ttttgtttaa ttcttttacc gttaaatcca ttttaggata
    16501 tggaatcgta attccttctt cgtcaaacct aactttaacc tcttctagta aatcaagata
    16561 aattgtccaa taatcttctg tcttgcacca aactctagtt gcaaatttaa tagcattatc
    16621 tccatgttct actactccta taaatggttc tggttcattt attattaatt tatgattatt
    16681 aacaatatct ttaagtactc ttctaacatg tagtatatca tcttcatacc ctacactaaa
    16741 agtcaaatca actcgtctag tattctttgc tgaatagttt attagactat catttgatac
    16801 agaaccatta ggtataagta tctgtttatt atctggagta accaattgtg tataaaaaag
    16861 acctatttgt tcaaccgttc ctccatgacc tgctgcttct acgtaatctc ctactttaaa
    16921 aggtctaatc aatagtataa taaatcctcc agcaaaattt gatagactac cttgtaaagc
    16981 caaacctatt gctacccctg ctgatgctat cacagcagca aaactggaca aactcattcc
    17041 ccaatattcc agaacaatga ttataacggc aactttaagt acactcaaaa gaagagactt
    17101 taaaaacttt cttaaagtta aatcaatatc tcttttacta agtaataaca taacatggtt
    17161 tacaaatttt tttattattt taaatccaat agaaagtata agtatgccta ttataagttt
    17221 tactccgctt gtagtagccc attctagcag actttccatt atacctccca tatccatctt
    17281 attaatatct aaattattta aatctaactt gcttatatca tctattttcg tcgtatccat
    17341 aaaaaactcc ttaccttata ttttagaacc aatattatag tttaaagttc ttattttcta
    17401 tttaatataa tacaatacca tttgtacata gtaaacaaga tttatattcc aattatacat
    17461 attaggaaaa tgcaaactat ttgttatata tatgcaaaat ataatactaa attaaactat
    17521 taataaaaca ctatatagcc ttaaaatcat cattttttac ccctatatga gatgatacta
    17581 tagttataat acttagtact ataaatacta caaaaccttt tttatatcca tcaactatat
    17641 tatatgaatt tacttgtaaa gagatagcta taatcaaatt aataattcca cttcctataa
    17701 attcaaaagt attaataaca ctagttgcca gtccagaata aatttcttta tttttatact
    17761 tggcatcatt aaaaacctgt agatgtgaca taactataca tcccataata aagaatgata
    17821 ttggtaaaac tataattggt ggttttacct ggtatacaac tacaattaca atccatataa
    17881 acaaatctat cattgctcca aattttatta tattaaattt gctagacctt attttagcaa
    17941 atacaaaatc cataattatt gaaccaaata taaatccata tgtaaaaaat gatactataa
    18001 acgcagaaaa actcttactc acaccataaa cgtccattat gtatcttact ccccacaagc
    18061 ttataaatgc acttgtaaga ccaacaaaag agaacattat cattgaatta taccatgttg
    18121 atttgttctt tataacagac ttaataccat caacaatatt aaccttctct gacttttcat
    18181 agggttcagt atctacattg aatccatact cttttggtgt atctctaaca ataatataca
    18241 taaaacatcc aattacaaca ccaattatac cgattaataa aaatgaattt ctccaaccaa
    18301 caagttcaga caaaaacaca agtggaaaag tagcaagaac tccacctaaa tttcctacaa
    18361 atgaaaattt agctgttgca gaagcaaatt ctgacttatt aaaccatctc ccttgaattt
    18421 ttaaaataca aagtaatatt actgatgttc ctgcccctac aattacacga gaaatatatg
    18481 ctaattctat actttgaatc aaaccaaata aaattgaccc taaacttgcc attattattc
    18541 caagactgct aatttttctt gctccatatt tatcaatcaa aattcctgca ggtatttgca
    18601 taaatgcata tgcatatagg caaaaggatg ctatatttga aatttgtata gaattaaaac
    18661 ctaactcatt tgctaaattg tctgaaacaa cagcagttga catccttaaa aataatacta
    18721 aaacaaatga aaatgtaatt ataccccaaa cactccactt agcctttgta taacttttca
    18781 tatcttctcc ctcaaatcca tatattttta aattttcttg atataactta tataaagtgt
    18841 atagttattc ttacaaattt ttacacgcaa tatcattata caacatatgt atatatttaa
    18901 ctagaaataa aacactattc atattatata catgaattaa ctacataaat aataagagcc
    18961 atcttttaac attactcaaa agatagctcc atataaaaac atttattcta aatttaattt
    19021 tttatctaat ataaattttg tagcgaaaaa taacgctatt gcaactacaa gactaccaag
    19081 tattgcaagc ataaaactag gactctgata aagatgatat accatatttg tatcttcatt
    19141 tggtaagatg cttccaactg catctccaac aattgatatt actatattta tagctataaa
    19201 agaagcaaat gccactatgt ttctatgttt attgaatttt ggcaattgtc ccattgagat
    19261 tgatgtatat aataacaaaa taaatgatga atatgaaata aacattacta ctactaataa
    19321 taaaatatct aaaaagtttt cagaaatcca tttaaaacta gtgttaaaca aatctaaaat
    19381 ttcaggtagt aaaattccag aagtaccaaa aagcataaga actccaaaag tgaatactgc
    19441 aactataaaa ctaagtacag cgtatataag tgctgttata catttagata atataagtga
    19501 actagtgcta acaggaagag taaacattaa ataaccttca tcccccaaaa gattttttct
    19561 aaacctttga attgtaacta caatagttaa aactcctaat gccataaaaa gacttgttaa
    19621 tactagtatt cctattccct gtacctgaag tatatttgta ttcataaata ttccattaaa
    19681 tacagctact atcaatatgg ctatatataa cggtataaat attcttccac ttgcttttaa
    19741 ctcatatttt aataatttac ctaacattta aattcctccc taaataatgc atctatagat
    19801 tttccttttt cttctcttat tgcttcaacg tctccttgta acactatttc tccttttgat
    19861 atgaatatta tctcatcaca tatgttttct atttcactta ttaagtgggt agcaatcagt
    19921 aaagtactat cttcagaata attttttaat atagtcttaa gtatatatga ccttgcagct
    19981 gggtcaacac cacctatagg ttcatccaat atatatatgc tcgcatttct tgacattact
    20041 aaaataagtt gaactttttc tttagttcct ttagacattg tctttagctt ttcatttaca
    20101 tcaatatcta agcttttaat catctcattt gctcttctaa catcaaaatc actataaaaa
    20161 tcatagaaaa acttaagtaa atctgatacc ttcatccaat catttaaata agttctttct
    20221 ggtaaatatg aaactatttt tttagtatct atggatggtt tcattccatt tatcataatt
    20281 tcaccattat caggctgtaa taatccattc attagcttaa tcattgtact tttgccactt
    20341 ccattaggtc ctaacagccc gactattttt ccctttggta tatttaaatc aatatttttt
    20401 aaaacattct tagttccata actcttgttt acacctttaa attcaactat tccatcttga
    20461 gaaacattgc ttatattttt atccatttta taattctcct ttcagatttt ctgtaataag
    20521 ttttagcatt tcatctctat catatccaag cttttctaag gttactttta atgtatttat
    20581 ctccacatta gctatttcct ctttcatagc ttttattttt tccttatcat ctgttacaaa
    20641 tcttccttta gttctctgac tataaactaa accttgtctt tcaagttctg ttagagcctt
    20701 ttgcattgta tttggattaa cttccgcatc ttctgctagt gctctcactg tttctaattt
    20761 agatcctatt ttaatttccc ctgaaattat ttttaacttt aaatgttcta ctaattgtat
    20821 atatataggt ttgttattat caagttccca ttccatgttc atgcctcctt attatattat
    20881 tgtattaagt tgttaataca ataatataat aatacaaagt atatgtcaat tactttttag
    20941 taaaagtata aaaaatttta caaaaaaaat gaacactaca ttagagtcca tttttaacac
    21001 aatttattaa attctttata ttacagatta attaccctct taccaagtta gatacaatta
    21061 aatgttcaaa ttcttcaatt ggcattggct ttccaaacaa atatccttgt gccatatcac
    21121 actttacttc ttttaaaagt ttaaactgtt cttctgtctc aactccttct gatatgactt
    21181 tcatacccaa ctcttttgcc atttctatta ctttagtaac aattattttg cttcttttac
    21241 tattataagt ttccataata aatttttggt caagttttaa gatatccata ggaatttctt
    21301 ttaacatatt taatgatgag taccctgaac caaaatcatc catagatatt aaaaatccta
    21361 ttttttttaa attattcatt atatcaatta aaatatcaaa attatcaaat atgatactct
    21421 ccgttaattc aagttctata tactttgcag gtatattgta tttatcaatt atatttttgt
    21481 atgtttctat aaaattattg caatataaat gtatccttga aacattaact gaaacagtaa
    21541 ctggatttat tcctgcatca atccattttc taaggtatat acatacttct tcaagcacat
    21601 ataaatctaa atttataaca aaaccgtttc tttcaaaaaa tgggataaat tcatttggag
    21661 gtattagccc tttatcagga cgtttccacc taacaagtgc ttctgaacct tgtatctctc
    21721 ctgtattaag ttctatctta ggttgtaagt atataataaa ttctttattt tctagacttg
    21781 aaaacattgt attttccatc tctttttctt ttatctcttg gtcatgaatc ttattatcat
    21841 aaaatgcata tgaactttta tgtcctccct ttgccatttt atgaggtata ttagccctat
    21901 ctatgattga gttgatatct ctatcttctg gaagtataaa atagattcca caacttaaaa
    21961 ctaacttata tttttcttct tgattattat taaacaagtc taattcttca aatatagttt
    22021 ttaacctctt tgttatatct tcttcttcta tatatttcat aattataata aaattatccg
    22081 cagatattct tgcaaacatt tcctcttcaa atatattgct cttaataatc tttgttaaat
    22141 tacgcaaaat ttcattccca acttcatatc caaataaatc atttatatat ttaaatttat
    22201 caatatctat atataataaa gcatacttta cttgagtatt tttacatagt attttgttag
    22261 catcaattac aaatttatct atagtatttg ctcctgtaag attatctgta tatgctatcc
    22321 tttgcaattt aattttaaaa tttaatttat catatattat atagaatata agtattacac
    22381 atattatagc cataataagt attatttgta ttgcatgttt gctaataagc tgattattag
    22441 acacaataga ctcaaagtta tttatggagt tgtttttata gagtttgcat aaagaataga
    22501 ctggtgttgt gtatgtaatt tgtaaaatta tacaaataaa taaaaaaaac atcaattttt
    22561 tttgtaaatt cctcataaaa atttcctccc attaaaacac ctctatttta ttagcaaata
    22621 agtacatctc aaaagtttcc atttcttgta aaatggatag tttatatcta ctttaatttt
    22681 gtaaaatttt ctgctactat aattatagaa tgtagcatat tcattgtctt aactattgat
    22741 ttatatattt catataggta taatagaaaa taaattggat tattttggca tataattatt
    22801 tattaaaagg tgattatttt tgtttaaatt gtctgaaaaa ttttattttt atattataat
    22861 tttaatattt tcttttc
//
LOCUS       DAMJVK010000064        22153 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.065, whole genome shotgun sequence.
ACCESSION   DAMJVK010000064 DAMJVK010000000
VERSION     DAMJVK010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22153
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.065"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            451..1521
                     /locus_tag="PC414_002487"
     CDS             451..1521
                     /locus_tag="PC414_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2464875.1"
                     /translation="MADIRLENVTKSYNKKNTVIENLNLTIEDGSFTVLVGPSGCGKS
                     TTLRMIAGLEDITSGRLKIEENDVTKTDASKRDIAMVFQNYALYPHMTVRENIEFGLK
                     NKKVEKEKREKLITEVIDVVGLREYLNVKPGNLSGGQRQRVALARAMVKNPKVFLMDE
                     PLSNLDAKLRNQMRTELIQLHQKLKSTFVYVTHDQVEAMSMADKIVIMNKGKIMQIGS
                     PKKIYQNPKNLFVAQFIGSPCMNILDIKNDIKFGFRPEKATLESINIDGKNDDVFSLF
                     GKVVTKEVLGNETIYCLSVLNREIRVKTENDIFDIGRTLRISVLEKDLYYFDKNGERI
                     EDVKVCKSMYSKIKGDIYEKAI"
     gene            1505..2374
                     /locus_tag="PC414_002488"
     CDS             1505..2374
                     /locus_tag="PC414_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDF2464876.1"
                     /translation="MKKQFNKAYLYITPAIIGTLLFIVYPIIKTISLSFKSGSLLSST
                     FENVGLNNYTELLKNPEFIQTITNTAIYTFFMVTISISLAIVLAVWLNKNSIIHNLTQ
                     SIIFTPHVISLVSVAVLWLWIMEPQYGFLNWILSTLHLPTSKWLSSESSALMSLILIG
                     IWKTLGYNILIVLSGLKSIPNYIYEASKLDNANKITNFFKVTLPLLSPTIFFLMITTT
                     TASFQVFDEISIMTQGGPVGSTNMLSYYIYESGFIYYDIGQASAASMFLLAIVMIATY
                     ANFRLLSKKVHYQ"
     gene            2418..3278
                     /locus_tag="PC414_002489"
     CDS             2418..3278
                     /locus_tag="PC414_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDF2464877.1"
                     /translation="MDIIQPIKKDKVDIKKIASKSIEILILLLLSAIFIFPFIWMLST
                     SFKNPQEMMQLPPTIIPNDIVLENYSTAWHSGPFFRYLLNSVFVTGSVLIVQFLVMVP
                     AAYAFSKIKFKGEKMLFGLLLVGLMIPPQVTFLPVYMMMSKFGFINTYVPLIIPFMTS
                     SFGIFLLRQNFMQISDEIIEAAKLDNASDLKIMFRIMVPMAKPAVITFILFNFIYHWN
                     NYFWPLVMTNTDAIRTLPIGVALLKSSEGITAWNVIMAGNIILILPIILVYIFANKKV
                     KEAFMYSGIK"
     gene            3309..4613
                     /locus_tag="PC414_002490"
     CDS             3309..4613
                     /locus_tag="PC414_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2464878.1"
                     /translation="MKLKRKLATGLAITLLLCSLVGCSNSNDANASKGEKTDSNITKI
                     DFWAPLGGTNGETAQKMVDQFNSEHKDVQVNMLKQKDYYENATKLQAALTSKDQPDVT
                     LLEITQTGTFASAGALVDMSKYFDKTYQERFFSGLLTNSYYEDKFVGMPFNRSTPILY
                     INKDMATKAGLDPSGPKSWEELKDYASKMTNKSEDTYGFETPIDIWFYEAMVMQSGGE
                     ITNGKKVAFNNEAGQAPVKFWQDMMKAGIMKMPPGEDYNAWDVAKQDFVNGKVGMIFT
                     STADLAGLMQQTEGKFEISTAFLPKNQKYATPTGGANLVMLEGGTDKEKEASAEFIEW
                     MTQTDKIVQFSSSTGYLPTTEDATNSEKLQTLYKEKPQYKVATDQLQYAVALPMLNGY
                     KEATDKLMDEIKKGLTDLNANPKDVVSKGTSAMQAVIDKTNK"
     gene            4676..5938
                     /locus_tag="PC414_002491"
     CDS             4676..5938
                     /locus_tag="PC414_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HDF2464879.1"
                     /translation="MTKVIVLSVDSLFEKDLEFVKNLPNFKSILENCSIVKNINCVYP
                     TLTYPCHTSMVTGVYPKKHGIYHNEKFDPNKENKDWYWYSKDIKAKTIIDVAKENNLT
                     TSSVLWPVMGANPNIDYNIAEIWAPSREDDPRETFERSSSKVIMDGIYNRHCHYIDWK
                     LEPNMDLFGVNCSVDIIKEYKPDLMLIHLATLDHTRHNYGLFNDEVDKALKMNDKWLG
                     DIIQATKDAGTYKDTNFIILGDHGHLRVDKVINPNVLLKSNGFIKVENGEVKDFDAYV
                     NSSGISAQIIVNNLDRLTELRELLEEFKEELFIENIFTKEEASNLGLEGDFEMVIEGL
                     EGISFGNDFEGSIIKDSDIKDYKFAVATHGHLPTKGNRPPFIAFGPNIKGGVVIEEGD
                     LRAHAATILKMFNLKLDGVEKEAFDFIM"
     gene            6125..8095
                     /gene="uvrB"
                     /locus_tag="PC414_002492"
     CDS             6125..8095
                     /gene="uvrB"
                     /locus_tag="PC414_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HDF2464880.1"
                     /translation="MDFKIKSDFKPTGDQPEAIKSIVDSINRNEKFSTLLGVTGSGKT
                     FTMANIIQQVKKPTLIMAHNKTLAAQLYSEFKEFFPDNAVEYFVSYYDYYQPEAYVAH
                     SDTYIEKDASINDEIDKLRHSATASILERRDTIIISSVSCIYGLGDPKDYKELMLSIR
                     PGMQRDRDDVIKRLIEIQYERNDINFTRGTFRVRGDILEIFPASNDEKAIRIEFFGDE
                     VDRITEIDYVTGKIVGTRNHVVIFPASHYVTTPERIEKAIVEIENELQEQIKFFKEND
                     RLLEAQRIEQRTKYDIEMLKEIGFCQGIENYSRHITGRSEGERPYTLMDFFPDDYLII
                     VDEAHVTIPQVRGMYAGDRSRKTSLIENGFRLPSALDNRPLNFQEFEGNINQMLFVSA
                     TPGPYEIQHSETIAEQIIRPTGLLDPIVEVRPINNQIDDLVGEITKTIEKNERVLITT
                     LTKKMSEDLTNYLKEIGIKVKYLHSDIVTLERTEIIRDLRLGKFDVLVGINLLREGLD
                     IPEVSLIAILDADKEGFLRSETALIQTIGRAARNENGRVIMYADRITDSMQNAIDETK
                     RRRDIQNLYNEEHNIIPKTIQKNIRDSIEATKVAEEEVVYGISDTDDKDEIRANIDKL
                     KSEMMEAAQNLQFERAAELRDKVKQLEEKLEK"
     gene            8114..10939
                     /gene="uvrA"
                     /locus_tag="PC414_002493"
     CDS             8114..10939
                     /gene="uvrA"
                     /locus_tag="PC414_002493"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDF2464881.1"
                     /translation="MEDKIIIRGAKEHNLKNIDLELPRDKFIVFTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGQMEKPNVEYIEGLSPAISIDQKTTSKNPRSTVGTV
                     TEIYDYLRLLFARVGDVYCPTCGEKISQMTIQEIVDKMLDFPDRTKLQILSPIVRGQK
                     GTHKKVFDNIKKEGFVRVRVNGENYEVSDDIILNKNQKHNIEVVVDRIVIKDGIESRL
                     ADSIETAVKLSDGLVIAQIIDGEEILFSTKFACPEHGIGIEELSPRMFSFNAPFGACD
                     VCNGLGESKEVDPELVVPNKDLSIKQGAVAAWGSTGVNDDTYYSKMIKSLAEHFNVSL
                     TTPFKDLPDDFVEELLYGKDNIIVEFTYESKFGGTRNYKSYFEGVIVNLERRYRETNS
                     DYMRDKIEEYMAERPCPKCKGMRLKKEVLSVLVDGKNIMEVTNLSVNELIDFMENINL
                     TEKQRFIAHEIIKEIKGRAMFLRDVGLDYLNLSRKAGTLSGGEAQRIRLATQIGSALV
                     GVLYVLDEPSIGLHQRDNDRLITTLRHLTDLGNTLIVVEHDEDTMREADYVIDIGPGA
                     GIHGGEIVAQGTLDEIIENENSITGQYLSGKKQILLPETTREGNGSFIEIVKASENNL
                     KNIDVKFPLGKFTCITGVSGSGKSTLINDILYKGVASKVNRLKQRAGKHKEIKGIESI
                     DKVINIDQSPIGRTPRSNPATYTGVFDFIRDIFASTNEAKARGYKKGRFSFNVKGGRC
                     EACKGDGIIKIEMHFLPDVYVPCEICKGERYNRETLQVKYKDKTISDILDMNVEEAVE
                     FFENIPNIKRKLETLMDVGLSYIKLGQPSTQLSGGEAQRIKLAAELSKRPTGKTLYIL
                     DEPTTGLHMADVDKLIQVLQRLADTGNTIIVIEHNLDVIKTCDYVIDLGPEGGDKGGT
                     IVATGTPKEVSKVDGSYTGQFLKKYFS"
     gene            11198..13015
                     /gene="uvrC"
                     /locus_tag="PC414_002494"
     CDS             11198..13015
                     /gene="uvrC"
                     /locus_tag="PC414_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HDF2464882.1"
                     /translation="MFDIQEHLKKLPSEPGVYLMKDKYDHIIYVGKAISLKNRVRQYF
                     QSSKNHTSKVKSMVKNIYKFEYIITDSELEALILECNLIKRYRPKYNVVLRDDKTYPY
                     IKVTTNEDYPRILKVRRVLKDKAKYFGPYTNITAVNDTLELISSTYPIRSCKIDIDKA
                     IKNKTRPCLNLHINKCLGPCTGNVSKEEYGKMIEEIIMCLSGKEEKLMELLKEKMNES
                     SMNFRFEEAAVYRDKIKSLEEMIQKQKIDATVSDLNQDVVAMARAHNEACVQVFFIRN
                     GKIVGREHFILEGVMDSPRASILSSFVKQFYNEQEYIPKELIIEDEIEDSSILEEWLS
                     SKKGQKVTIRVPQKGEKKSLVEMVRKNAVEYLEKFSDMNKRKYEKSIGALEELKQILN
                     LEKLPIRIEAYDISNIQGVDSIGSMVVYTNAKKDKKEYRRYKIKTVIGPNDYDSMAEI
                     VDRRLKHGNLPDLILLDGGKGQVSAVKKVLELNDVDIPLWGMYKDDKHRTKGLICKEK
                     EIELDKTTNLYRFIASIQEEVHNYAITYHRSLRNKVLTKSILDDIQGIGEKRKKSLLN
                     HFKDVDAIKKATMEELLEVDGMNKSIADNVYNFFRKEEN"
     gene            13015..13944
                     /locus_tag="PC414_002495"
     CDS             13015..13944
                     /locus_tag="PC414_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HDF2464883.1"
                     /translation="METSNKVSIREIIKELKLKVVYMPDDVDYYVYSQDINRPGLQFA
                     GYFDYFAYERIQIVGKTEYNFFGLMDSHIREEVLDRFFSYEIPALIISRDLEIKPDVI
                     EKAKKYKRILLSTNRNTTRLINRLSNYLDNKLAPHTTIHGVLVDIYGIGVLIKGESSI
                     GKSETALELIQRGNRLVADDAVEIRKLDESMLVGQSPELIRHFLEIRGIGIIDVRSLY
                     GVGAIKNSKMIDLVVHLEAWDETKYYDRLGLDKEYEEILGVNVEKLVVPVKPGRNTAM
                     ILEVAAMNFRQRGMGYDAAQEFTKKLSKLIDTK"
     gene            14156..15952
                     /locus_tag="PC414_002496"
     CDS             14156..15952
                     /locus_tag="PC414_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate oxidoreductase"
                     /protein_id="HDF2464884.1"
                     /translation="MGIEYFSKLLESSTEKELLLSKKSNNISTARLISFLIVIAGLAI
                     GFYNKNIVGIFVGVLSIIIFIALLVIHSKVKEEETYFKSKSEVLNKYIKRFGDGWKEF
                     KIDGKEYLKDENSQAKDLDLFGRASLYQYICVANTSYGKNFLAKYLWNENPDENIILE
                     RQRAIKELLSKDDFSIHIQTLSNIIGKEQKNNSDSSIESFIEYGENKNVYIPKWMHIF
                     TWGLPTATILSFIFCMLGFLPVLPVFLLFIIQLGFSGFGYPRLMQTLAPLFSFSRSIQ
                     VYEKMFEVLEKETFESAYLKELQDKLSKGSGVSRGVKQLNSIGNAVNLRYNQILYIIA
                     CGVLMWNYHCAEALERWKGIYGNQIRDWFESIGEFEALISLTVVSHVKENTCFPVIKY
                     EDTPRLKVEEVYHPLIAEKSVIANSIQLNSQTCIITGSNMSGKTTFLRSIGVNLVLAY
                     AGAPVCAKSFDATCMAIFTSMRIQDDVSQGISTFYAEILRIKSMIQYSLKELPMLVLV
                     DEIFKGTNSADRIIGASEAVKKLSKPWVISMVTTHDFELCDLSGSGDVEVVNYHFSEY
                     YVDDKIHFDYKIKDGRCKTTNAKQLMRMAGIL"
     gene            complement(16092..17873)
                     /locus_tag="PC414_002497"
     CDS             complement(16092..17873)
                     /locus_tag="PC414_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HDF2464885.1"
                     /translation="MSLVNLTINGKHVSAPSGTSILDAAKLINIKIHNLCHLHMNEID
                     KLDTCASCRVCMVETERGLVPACGTVIKEGMKVQTNSAKALNARRTIVELLLSDHPQD
                     CFVCEKNGDCELQTIAADLGVRKIRYQGSKSFAGKDTSTKSLVKDHSKCILCRRCETV
                     CNDIQTVGALSGVNRGFNTLVSTFFNSDMVETECTFCGQCISVCPTGALTEVDNVPKL
                     WDVLNKKEKTIVVQVAPAVRVAIGEEFGLEPGSISTGKMVAALKALGFEHVFDTNFGA
                     DFTIMEEATEFIERIQKGENLPILTSCCPAWVNFLEHNYPDKLNLASSCKSPQGMFGS
                     IAKNYYAPKILGINPEELYVVSVMPCVAKKYEASREELSESGILDVDLSITTRELAKM
                     IKEAAIDLPNLQDQDFDNPLGKSTGAASIFGASGGVLEAALRTSYEKITNKTLDNVNF
                     TNVRGLKGIREASIDVDGTTVNVCIVNTLKNARKIMDKVRSGECKYHIIEVMACPGGC
                     VGGAGQPYHHGNTEIVDRRANALYEIDRNKAIRKSHENPDLQAIYKDFFGEPNSDVAH
                     KYLHTHYFDKSCVYGECPQECACEEAK"
     gene            complement(17896..19782)
                     /locus_tag="PC414_002498"
     CDS             complement(17896..19782)
                     /locus_tag="PC414_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HDF2464886.1"
                     /translation="MRKVNSFDELKVLADELKPNLSLRKNYSKENVLRRELLVCCDTG
                     CTSSNSLEIVSELENEIKKSGIQDKVSVRLTGCFGFCAQGPIVKVYPDNVFYVKVEPS
                     DAEKIVQSHLIRNTVVEELLYEEESLSKKVEEQEEMSFYKKQLRIALKNCGLIDPDSI
                     EEYIANDGYLALGKCLTSLTPQEVIAEVKTSGLRGRGGAGFPTGSKWEAASKNPAGPT
                     DKKFVVCNADEGDPGAFMDRSVLEGDPHSVLEAMAICGYAIGSDTGYIYIRAEYPKSI
                     ERLKVAIAQAEKYGLLGENILGTGFNFKLELKYGAGAFVCGEGTALMHSIEGRRGEPR
                     MKTYSSSKSGLWKSPTCLNNVETFANIPAIILKGGDWFANLGTEDSSGTKVFALGGKV
                     ENVGLVEVPMGTTLRDIVFEIGGGIPEGKKFKAVQTGGPSGGCIPTEHLDTPIDFGSL
                     SSIGSMMGSGGMLVLDESDCMVDIAKFFLEFTVEESCGKCTPCRIGTTRLLEILTKIT
                     EGNGTLQDLDDLENLAETIQTASLCGLGKAAPNPVLSTLQYFKDEYIAHVVDKKCPAG
                     KCQNLLSYFITDDCKGCTKCSRVCPAGCITGSVKEQHTIDTSKCLKCGACIDNCTFNA
                     IIKK"
     gene            complement(19799..20296)
                     /locus_tag="PC414_002499"
     CDS             complement(19799..20296)
                     /locus_tag="PC414_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HDF2464887.1"
                     /translation="MCDFISHNKQLFDELDIFIDSLATKEGALIQVLHEAQGIFGYLP
                     KEVQLHVARKLGVAPAKVYGVVTFYSYFTTEPVGKYKISVCLGTVCFVKGADKILSAF
                     EKQLGIKVGETTSDFKFSLEGLRCLGACGLAPVVTVNGKVYGKVKPDQVSEILDTYRE
                     LELNC"
     gene            complement(20614..22095)
                     /gene="cls"
                     /locus_tag="PC414_002500"
     CDS             complement(20614..22095)
                     /gene="cls"
                     /locus_tag="PC414_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDF2464888.1"
                     /translation="MFDISFFEMSIYEIAVTTIYIINFMVILNLIFREKRNINTTLTW
                     LLILVLIPALGFILYIAFGRNISKNNMFRLKEKDDKIIKSNILDTQVKLQSTSEIDSD
                     IHQHKDMIYALANSNNAHYTNNNDVWIYAESSQFFNSLLEELKKAKKYINIQFYIFKD
                     DKIGTEIIDILIDKAKEGVEVRLLFDAVGGRTLKNSTLSRLKESGVKVGSFFPSFLKI
                     VNFNLNYRNHRKIVVIDGKVGFVGGYNVGDEYLGRNPKFGLWRDTHTKLTGDCVIDLN
                     MRFILDWRYTTKEDLDLEKYFIEEDTNSNHPSENIGIQIVSSGPDISELDEIKYGYLK
                     MIQKARKYIYIQSPYLILDSTFIDTLKIACLSGVDVRVMIPSKPDHPFVYWASYSYAG
                     ELLKFGAKIYTYGQNAFLHAKTIVIDDSICSIGTANMDIRSFELNFEVNAFMYSSKKA
                     LEQRIIFENDILNSKEITLDIYNSRSTYIKIKESISRLLSAVL"
BASE COUNT         8520 a         2969 c         3696 g         6968 t
ORIGIN      
        1 ttttttgggt aaatttataa aaattaaaaa atttttattt tttatgttta aaccttgtaa
       61 atttgggaca taatataatt gtaaaactta atatagcaaa aataaattac tcaatctccc
      121 ccacttaaag acctctatcc cccatagagg tcttttttac tttcaaacca aattatataa
      181 aaaataaata gttttatatg actcttcata aattaaaatt acattgtgat agcaaaccat
      241 gaatatgaaa aatatagaaa attatagcta acataagctg tatatcatct taacttcagt
      301 atatttaaca aaaaatgttt ataatttaac ctttatttta ataagtttac catatattca
      361 ctttgtctta atatatcctt tacaaattaa gagtattatt aatctataag taaatcgtag
      421 aattaaatta aatattttgg gaggataaaa atggcagata taagactaga aaatgtaaca
      481 aaatcttata ataaaaaaaa tacagttata gaaaatttaa atcttactat agaagatggt
      541 tcttttacag tattggtagg cccaagtgga tgtggtaaat ctactacgtt aagaatgatt
      601 gcaggtcttg aagatataac ttcaggtagg ttgaagattg aagaaaatga tgttacaaaa
      661 acggatgcta gtaaaaggga tatagctatg gtatttcaaa attatgcatt atatccacat
      721 atgactgtta gagaaaacat agaatttggt cttaaaaaca aaaaagtaga aaaagaaaaa
      781 agagaaaaac ttataacaga agttattgat gtagtaggat tgagagaata tttaaatgtg
      841 aaaccaggca acttatctgg aggtcaaaga caaagagtgg cacttgctag agctatggtt
      901 aaaaatccaa aggtattttt aatggatgaa ccattgtcaa atttagatgc taaattaaga
      961 aatcaaatga gaacagaatt aatacaatta catcaaaaac taaagtctac atttgtttat
     1021 gtaactcatg accaggttga ggcaatgtca atggcagata agatagttat aatgaacaaa
     1081 ggaaagataa tgcaaatagg ttctccaaag aagatatatc aaaatcctaa aaatttattt
     1141 gtagctcaat ttatagggag tccttgtatg aacatactag atataaaaaa tgatattaaa
     1201 tttggattca gaccagaaaa agcaacacta gagagtataa acatagatgg gaaaaatgat
     1261 gatgtattta gtctatttgg aaaagttgta actaaagaag tgttgggaaa tgaaactata
     1321 tattgtttat ctgttcttaa tagagagata agagtcaaaa cggaaaatga tatatttgat
     1381 attggaagaa ctttaagaat aagtgtatta gaaaaagatt tatattattt tgataaaaat
     1441 ggagaaagaa tagaagatgt aaaagtatgt aaaagtatgt attctaaaat caaaggagat
     1501 atttatgaaa aagcaattta ataaagcata tttatatata acacctgcta ttataggaac
     1561 tttattgttt atagtctatc caataattaa gacgatatct ctaagcttca aatctggtag
     1621 tcttcttagt tcaacctttg aaaatgtagg cttaaacaat tatacagaac ttttaaagaa
     1681 tccagagttt atacaaacta taacaaacac tgcaatttat acttttttta tggtgactat
     1741 atctatatca ctagccatag tacttgctgt ttggctaaat aaaaattcta taatacacaa
     1801 tttaacgcaa agtataatct ttacaccaca tgtaatttca ctagtatctg tagctgtatt
     1861 gtggctttgg attatggagc cacaatatgg ctttttaaac tggatactat cgacattaca
     1921 tttaccaaca tcaaagtggc tttcaagtga aagttcagct ttaatgtcac taattctaat
     1981 aggaatatgg aagactcttg gatataatat cttaatagtg ttatcaggtc ttaaatccat
     2041 accaaactat atatatgaag catctaaatt agataatgca aacaaaataa cgaatttctt
     2101 taaagtgaca ttgccactat tatcaccaac aatatttttt cttatgataa caacaacaac
     2161 tgcatctttt caagtatttg atgagataag tataatgaca caaggtgggc cagtgggttc
     2221 aactaacatg ttatcttatt atatatatga gtctggattt atatactatg acatagggca
     2281 agcgagtgca gcgagtatgt ttttgttagc tatagttatg attgctacat atgctaactt
     2341 tagactctta tcaaagaaag ttcattatca ataaagaaaa agtagatatt ataaggttta
     2401 tccaaaagga ggagacaatg gatattatac aacctatcaa aaaagacaaa gttgatataa
     2461 aaaaaatagc atcaaaatca atagaaatat taattctatt acttttatct gcaatattta
     2521 tattcccttt tatatggatg ctatctacat cctttaaaaa tccacaagaa atgatgcaat
     2581 taccgcccac tattatacca aatgatatag tgttagaaaa ttatagtaca gcatggcatt
     2641 caggtccatt ttttagatat ttgctaaaca gtgtatttgt aacaggaagt gttttaattg
     2701 tacaattttt agttatggtt ccagcagcat atgcattttc taagattaaa tttaaaggtg
     2761 aaaaaatgct atttgggctt ttgcttgtag gacttatgat tccaccacaa gtaacttttc
     2821 taccagtcta tatgatgatg agtaagtttg gatttataaa tacttatgtt ccacttataa
     2881 ttccatttat gacatcttca tttgggatat ttctgcttag acagaatttt atgcagattt
     2941 cagatgaaat tattgaagca gctaaactag ataatgcttc tgacttaaaa ataatgttta
     3001 gaataatggt tcctatggca aaaccagcag taataacatt tatattattt aattttatat
     3061 accattggaa taattatttc tggcccttag taatgacaaa tacagatgca ataagaactt
     3121 taccaatagg agttgcgctc cttaaatctt cagaaggaat tactgcatgg aatgtaataa
     3181 tggcaggaaa tataattcta attttaccaa taattcttgt ttatatattt gcgaataaaa
     3241 aagtaaaaga agcttttatg tattcaggaa taaaataaaa aattaaagac taaatttgga
     3301 ggatgaaaat gaagcttaaa agaaaattag caacaggatt agcaataaca ttattattat
     3361 gttctttagt tgggtgttca aattctaatg atgcaaatgc atctaaagga gaaaagactg
     3421 atagcaatat tacgaagata gatttctggg ctcctttagg tggaactaat ggagaaactg
     3481 ctcaaaaaat ggttgaccaa tttaatagtg aacataagga tgtacaagta aatatgttaa
     3541 aacagaaaga ttactatgaa aatgcaacaa aactacaggc agccttaaca tctaaagacc
     3601 aaccagatgt aacattatta gaaataactc aaacaggtac attcgcatca gcaggtgctt
     3661 tagtagatat gagtaaatat tttgataaaa catatcaaga aagattcttt tctggtcttt
     3721 taacaaattc atattatgag gataagtttg ttggtatgcc atttaataga agtacaccaa
     3781 tattatacat aaataaagat atggcaacta aagcaggtct tgacccttca ggtccaaaat
     3841 catgggaaga gcttaaggat tatgctagta aaatgacaaa taaatcagaa gatacttatg
     3901 gttttgaaac accaatagat atttggttct atgaagctat ggtaatgcaa agtggaggag
     3961 aaataactaa tggtaaaaaa gtagcattta ataatgaggc aggacaagca ccagttaagt
     4021 tctggcaaga tatgatgaaa gctggaataa tgaaaatgcc tcctggagaa gattataatg
     4081 cctgggacgt agcaaaacaa gattttgtaa atggtaaagt aggtatgata tttacatcaa
     4141 cagcagattt agcaggactt atgcaacaaa cagaaggtaa gtttgaaata tcaacagcat
     4201 tcttaccaaa aaatcaaaaa tacgcaactc ctacaggtgg agctaatcta gttatgcttg
     4261 aaggtggaac agataaagaa aaagaagcaa gtgcagaatt tatagaatgg atgacacaaa
     4321 cagataaaat cgttcagttt agttcatcaa caggatactt accaacaact gaagatgcaa
     4381 caaactcaga aaagttacaa acattatata aagaaaaacc tcaatataag gttgcaactg
     4441 accaattaca atatgctgtt gcacttccaa tgttaaatgg atacaaggaa gcaactgata
     4501 agctaatgga tgagattaaa aaggggctta ctgatttaaa tgcaaatcca aaagatgtag
     4561 taagcaaagg aacttctgca atgcaagcag taatagataa aacaaataaa taattatata
     4621 gacttgtaag ttgattaaaa gtattcaata atatataaat gaagaggtaa tgtaaatgac
     4681 aaaggttata gtattatcag tagattcttt atttgaaaaa gacttggaat ttgtaaaaaa
     4741 tctaccaaac tttaaatcta tactagaaaa ttgctccata gtaaaaaata taaattgtgt
     4801 ttatcccact ctgacatatc cgtgtcatac tagtatggtt acaggagtat atcctaaaaa
     4861 acatggtata tatcataatg aaaaatttga cccaaataaa gaaaataaag actggtactg
     4921 gtactcaaag gacataaaag caaaaactat tatagatgta gcaaaagaaa ataatctaac
     4981 tacctctagt gtattatggc ctgtaatggg agcaaatcca aatatagatt ataatattgc
     5041 agaaatatgg gctcctagta gagaagatga cccaagagaa acttttgaac gaagttccag
     5101 taaggttata atggacggta tttacaatag acattgtcat tatatcgatt ggaaattaga
     5161 acccaacatg gatttatttg gagtaaactg ttcagtagat ataataaagg aatataaacc
     5221 agatttaatg ctaatacatt tagctacatt ggaccataca cgacacaatt atggcttatt
     5281 taatgacgaa gtagataaag cattaaaaat gaatgataaa tggctaggag atattattca
     5341 ggctacaaag gatgcaggta catacaaaga tacaaatttt ataatacttg gggaccatgg
     5401 tcatcttaga gttgataaag ttataaatcc aaatgtcttg cttaaatcaa atggatttat
     5461 aaaagttgaa aatggagaag ttaaggattt tgatgcctat gtaaattctt ctggaatatc
     5521 agctcaaata atagtaaata acttagatag gttaacagag ttaagagaac tgcttgaaga
     5581 atttaaagaa gaattattca tagagaatat ttttaccaaa gaggaagcat ctaaccttgg
     5641 tcttgaaggt gattttgaaa tggtaattga aggtcttgaa ggaatttcat ttggaaatga
     5701 tttcgaagga agtattataa aagattcaga tataaaagat tacaagtttg ctgttgcgac
     5761 acatggtcat ttacctacta aaggtaatag acctccattt atagcttttg gaccaaatat
     5821 taaaggtggt gtggttatag aagaaggaga cctgagagca catgcagcaa ctatattaaa
     5881 aatgtttaat ttaaaattag atggagttga aaaagaagca tttgatttta ttatgtaagt
     5941 tgaataaagt atttattttc catgaattat agacttctat tgcaaagata gaagtctttt
     6001 tttatgtttt agatttaagt aaaatttaaa tgggtaaaat atttaaaata acttaaacaa
     6061 ttttataaag gtatgatata ataatacatg tcaggaacta atgttcgaaa aaaggagtga
     6121 tactatggat tttaaaataa aatcagactt caaacctaca ggtgaccaac cagaagctat
     6181 taaatcaata gtggattcta taaatagaaa tgagaaattt tcgacattat taggtgtaac
     6241 gggttcagga aaaacattca caatggcaaa tatcattcaa caagttaaga aacctaccct
     6301 aataatggct cataataaaa ctctagcagc acaactttac agtgagttta aagaattttt
     6361 cccagacaat gcagttgaat actttgtaag ttactatgat tattatcaac cagaagcata
     6421 tgtagctcac agtgacactt atatagaaaa agatgcaagc ataaatgatg aaatagataa
     6481 acttcgacat tctgctacag catctatttt agagagaaga gatactataa taatatcatc
     6541 tgtatcttgt atatatggac ttggtgaccc aaaagactat aaagaactta tgttatctat
     6601 aagaccaggt atgcaaagag acagagatga tgtaataaaa aggttaatag agatacaata
     6661 cgaaagaaat gacataaact ttacacgagg tacctttaga gttagaggag atatactaga
     6721 aatattccca gctagtaatg atgaaaaagc aataagaata gaattttttg gtgatgaggt
     6781 agatagaata acagaaattg attatgtaac agggaagatt gttggtacta gaaatcatgt
     6841 tgttatattc ccagcatctc actatgtaac aacgccagaa agaattgaaa aggcaattgt
     6901 agaaatagaa aacgagcttc aagaacaaat taaatttttc aaggaaaatg ataggctatt
     6961 agaagcacaa agaattgaac aaagaactaa gtatgatata gaaatgttaa aggaaatagg
     7021 attttgtcag gggatagaga actattctag acatattaca ggaaggtcag aaggagaaag
     7081 accatatact ttaatggatt tcttcccaga tgattatttg ataattgtag atgaggcaca
     7141 tgtcactata ccacaagtaa gaggtatgta tgcaggagac aggtctagaa aaacatcact
     7201 tatagagaat ggttttagat taccttctgc acttgacaat agaccattaa attttcaaga
     7261 atttgaagga aatataaatc aaatgttgtt tgtaagtgct acaccagggc cttatgaaat
     7321 tcaacactca gagacaatag cagaacagat aataagacca actggccttt tagacccaat
     7381 tgtagaagta agacctataa ataatcaaat agatgatttg gtaggagaaa taactaaaac
     7441 tatagaaaaa aatgaaagag tgcttataac cactttaact aaaaaaatga gtgaggactt
     7501 gactaactat cttaaagaaa tagggataaa agttaaatat ttacactcag atatagttac
     7561 attagaaaga acagaaataa taagagattt aagattaggg aagtttgatg tccttgtagg
     7621 aataaactta cttagagaag gattggatat accagaagtt tcacttatag caattttaga
     7681 tgcagacaaa gaaggtttct tacgttccga aacagcttta atacagacta ttggacgtgc
     7741 agctagaaat gaaaatggaa gagtaattat gtatgcagac aggataactg actcaatgca
     7801 aaatgctatt gatgaaacaa agagaagaag agatatacaa aatttatata atgaagagca
     7861 caatatcatt cctaaaacta tacagaaaaa tataagagat agtatagaag ctactaaggt
     7921 tgcagaggaa gaagttgtat atggaatatc tgatacagat gataaagacg aaataagagc
     7981 taatatagat aaattaaaat cagagatgat ggaagctgct caaaacttac aatttgaaag
     8041 agcagcagaa cttagagata aagtgaaaca attagaagaa aaattggaaa aatagaaagg
     8101 taaaaacaac attatggaag ataaaattat aataagagga gcaaaagaac ataatttaaa
     8161 aaatatagat ttagaattac caagagataa atttattgtt tttacaggtt tgtcagggtc
     8221 tgggaagtct tctttggcat ttgatacaat atatgcagaa ggacagagaa gatatgtaga
     8281 aagtctatct gcttatgcaa gacaattttt aggtcagatg gaaaagccaa atgtagaata
     8341 tatagagggt ttatcaccag ctatctcaat tgaccagaag actacctcaa aaaacccacg
     8401 ttctacggtt ggaacagtaa cagaaatata tgattatttg agactattat ttgcaagagt
     8461 aggagatgtg tattgcccta catgtggtga aaaaataagt cagatgacta ttcaagaaat
     8521 agttgataaa atgctagatt ttccagatag aacgaaatta caaattcttt caccaattgt
     8581 tagaggacaa aaaggtacac ataaaaaagt ctttgataat atcaaaaaag aaggatttgt
     8641 aagagttaga gttaatggtg aaaactatga agttagtgat gatataatct taaataaaaa
     8701 tcaaaaacat aatatagaag ttgtagtgga cagaatagta attaaagatg gaatagaatc
     8761 aagactagca gattcaattg aaactgcagt taagctatct gatggtcttg taatagccca
     8821 aataatagat ggagaagaaa ttcttttttc aactaaattt gcatgcccag aacatggaat
     8881 tggcatagaa gaattatctc caagaatgtt ttcatttaat gctccctttg gtgcttgtga
     8941 tgtttgtaat ggtcttgggg aaagtaaaga agttgaccca gaattagtag taccaaacaa
     9001 agatttatca ataaaacaag gtgcagtggc agcatggggt tcaacaggtg ttaatgatga
     9061 cacttattac agtaaaatga taaaaagttt agcagagcat tttaatgtat ctttgacaac
     9121 accgtttaaa gatttaccag atgattttgt ggaggaatta ctttatggta aagataatat
     9181 aattgtagag tttacttatg agtcaaaatt tggaggaact agaaattata aatcatactt
     9241 tgaaggtgta atagtaaact tagagagaag atatagagaa actaattcag attacatgag
     9301 agataagatt gaagaataca tggcagagag accatgtcca aagtgtaaag ggatgagact
     9361 taaaaaggaa gttttatctg ttttagttga tggaaaaaat ataatggaag ttacaaactt
     9421 atctgtaaat gaattgattg actttatgga aaatataaat cttacagaaa aacagagatt
     9481 tatagctcat gagattataa aagagataaa aggaagagct atgttcttaa gagatgtagg
     9541 tcttgattat ttaaatttat caagaaaagc tggaacttta tcaggaggag aagcgcaacg
     9601 tataagatta gctactcaaa taggttctgc attggttgga gttttatatg tacttgatga
     9661 gccaagtata gggcttcacc aaagagataa tgataggctt ataactactc ttagacatct
     9721 aacagattta ggaaatacct taatcgttgt agagcatgat gaagacacaa tgagagaagc
     9781 tgattatgtt atagacattg gtccaggtgc aggtatacat ggtggtgaaa tagttgcaca
     9841 gggtacttta gatgaaataa ttgaaaatga gaactcaata acgggtcaat atttaagtgg
     9901 taaaaaacaa atattgcttc ctgagactac tagagaagga aatggaagtt ttatagaaat
     9961 agttaaagca tcagaaaata atctgaaaaa tattgatgta aagttcccat taggtaagtt
    10021 tacatgcata acaggtgttt caggttctgg aaaaagtaca ttaataaacg atatacttta
    10081 caaaggtgtt gcaagtaaag tgaatagact taagcaaaga gctggtaagc ataaggagat
    10141 aaaaggaatt gaaagtatag ataaagttat aaatatagac caaagtccta ttggaagaac
    10201 acctcgttca aatcctgcta cttatacagg tgtatttgat tttataagag atatttttgc
    10261 atctactaat gaagctaaag caagaggtta taaaaaaggt agatttagtt ttaatgtaaa
    10321 aggtggtaga tgtgaagctt gtaagggtga tggaataata aaaatagaaa tgcatttctt
    10381 accagatgta tatgttcctt gtgaaatttg taaaggtgaa agatacaata gagaaacatt
    10441 gcaagtaaaa tacaaagata aaactatatc tgatatatta gatatgaatg tggaagaagc
    10501 tgttgagttt ttcgaaaata taccaaatat aaaaagaaaa ttagaaactt taatggatgt
    10561 aggactttca tatattaaat taggacagcc ttcaactcaa ttatcaggag gagaagcaca
    10621 acgtataaaa ttagctgctg aacttagtaa aagaccaaca ggaaagactt tgtatatatt
    10681 agatgaacct actactggac ttcatatggc agatgtagac aagctaatcc aagtattaca
    10741 aagactagca gatacaggaa atacaattat agtcatagaa cataatttag atgttataaa
    10801 gacttgtgat tatgtaatag acttaggacc agaaggcgga gataagggtg gaactatagt
    10861 tgcaactgga acaccaaaag aagtatctaa agttgatggt tcttatacag gtcaattctt
    10921 aaagaaatat ttttcttaag ttatataaat tgtgaaaaat tttagagcag atatacgata
    10981 aactgttttg ttataagtaa attagtcaaa tataagttgt aaatagtaat acaaagtaaa
    11041 gtccatatta ctcttcatgg ttaacattgt tttcaaatca attatagaag ggaatatggg
    11101 ctttaacttg tttaaagcac tggaaattgt ataacttttt aaaattcata ttttagggta
    11161 taattttagt ataaaattta atgttttgga gggcgagatg tttgatatac aagagcattt
    11221 aaaaaaatta ccttctgagc ccggagtata tttaatgaaa gacaaatacg accatataat
    11281 atatgtggga aaagccatat ctctaaaaaa tagggtaagg cagtattttc aatcttcaaa
    11341 aaaccataca tctaaagtaa aatctatggt taagaatata tataagtttg agtatattat
    11401 aacggattca gagttagagg cattaatact tgaatgtaat ttaataaaaa gatacagacc
    11461 aaagtataat gtagtgctta gagatgataa aacttatcca tatataaaag taactacgaa
    11521 tgaagattat ccaagaatct taaaagttag aagagttctt aaagataagg caaagtattt
    11581 tgggccctat accaatataa ctgctgtgaa tgataccttg gagcttataa gtagtacgta
    11641 tccaataaga agctgtaaaa tagatataga taaagcaata aagaataaga caagaccttg
    11701 tttaaatcta cacataaata aatgtttagg accttgtaca ggaaatgttt ctaaagaaga
    11761 gtatggaaaa atgatagaag agattataat gtgtttatct ggtaaagaag aaaaattgat
    11821 ggaactttta aaggaaaaaa tgaatgagag ttctatgaac tttagatttg aagaggctgc
    11881 tgtatatagg gataagataa agagccttga agagatgata cagaaacaaa aaatagatgc
    11941 tactgtaagt gacttaaacc aagatgtggt tgcaatggca agagctcata atgaagcttg
    12001 tgtccaggta ttttttataa gaaatggtaa gattgtaggt agggaacatt ttatattaga
    12061 aggggtaatg gatagtccaa gagcttctat attaagttca tttgtaaaac aattttataa
    12121 tgaacaagag tatataccta aagaattaat tatagaagat gagatagaag atagttctat
    12181 attagaagaa tggctgtcat ctaaaaaggg gcaaaaagta acaataagag ttcctcaaaa
    12241 gggagagaag aaaagcttag ttgaaatggt taggaaaaat gctgtagaat atcttgaaaa
    12301 attttctgat atgaataaaa gaaaatatga aaaaagtatt ggagcattgg aagaattaaa
    12361 gcaaatatta aatctagaaa agttgccaat aagaatagag gcttatgata tatcaaatat
    12421 acaaggtgta gattcgattg gttctatggt agtgtatacg aatgctaaaa aagataagaa
    12481 ggaatatagg agatataaaa taaagacagt tattggacct aatgattatg attctatggc
    12541 tgaaattgta gatagaagat taaaacatgg aaatttacct gacttgatat tgttggatgg
    12601 tggaaaagga caagttagtg cagtaaaaaa agtactagaa ctcaatgatg tagatatacc
    12661 tctttgggga atgtacaaag atgataagca tagaactaaa ggattgatat gcaaagaaaa
    12721 agaaatagaa ctagataaaa caactaattt atatagattt attgctagta tacaagaaga
    12781 agttcataat tatgcaataa catatcatag aagtctaaga aataaagttt tgacaaaatc
    12841 tatcttagat gatatacaag gaataggtga aaaaagaaag aaatcattat taaatcactt
    12901 taaagatgta gatgctataa aaaaagctac tatggaagaa cttttagaag tagatggaat
    12961 gaataaaagc attgcagata atgtttataa tttttttaga aaggaagaaa attaatggaa
    13021 actagcaata aagtatcaat aagagagata ataaaagagt tgaagttaaa agtagtatac
    13081 atgcctgatg atgtagatta ttatgtctac tctcaagata taaatagacc aggattacag
    13141 tttgcaggat attttgatta ttttgcatat gaaagaatac aaatagttgg aaagacagaa
    13201 tataattttt ttggattaat ggatagtcat attcgggaag aagtactaga tagatttttc
    13261 tcatatgaaa taccagcgtt aataatttct agagacttgg aaatcaagcc agatgtaatt
    13321 gaaaaggcta aaaaatataa aagaatactt ttgagcacaa atagaaatac cactagactt
    13381 ataaatagac tgtcaaacta tttggataat aagttagcac cacatacaac aattcacggt
    13441 gtgctagtag atatttatgg tataggtgta ctgataaaag gtgaaagtag tataggaaag
    13501 tcggaaacag cattagaact gattcaaagg ggtaatagat tagttgcaga tgatgctgta
    13561 gaaataagaa aattagatga aagtatgctt gtgggtcagt cacctgaact aataagacat
    13621 tttcttgaga taagaggaat aggaataata gatgttagaa gtttatatgg agtaggggca
    13681 atcaaaaatt ctaaaatgat agatttagta gttcatttag aagcttggga tgaaactaag
    13741 tattatgata gactaggact ggataaagaa tatgaagaaa tacttggtgt taatgtagag
    13801 aaattagttg tacctgttaa acctggaaga aatactgcta tgatattaga agtagcagca
    13861 atgaatttta gacaaagagg aatgggatac gatgcagctc aagaatttac taaaaaatta
    13921 tcaaaattaa tcgatactaa gtagtactga ataatagtgg actacacatc atttaattaa
    13981 atattgatta ggattatgtc ctacacctta taaattaaaa tcaagttgta tatataatgt
    14041 ttattaataa gatatattat ataaggataa tttaaagtac agtgttagat tataataata
    14101 ctagattaat tttatatttg agtttcatta tataacaaga aagttggaat tttttatggg
    14161 aatagaatat tttagtaaac ttttagaaag tagtactgaa aaagaattgc tcttaagtaa
    14221 aaagtcaaat aacataagta cagcaagatt aatatctttt ttgatagtga tagcagggct
    14281 tgctattgga ttttataata aaaatatagt aggtattttt gttggagttt tatcaataat
    14341 aatatttata gcactcttag ttattcatag taaagtaaaa gaagaagaaa catattttaa
    14401 aagtaagagc gaagtattaa ataagtatat taagagattt ggagatggtt ggaaggaatt
    14461 taaaatagat ggtaaagaat acttaaaaga tgaaaattcg caagcaaaag atttagactt
    14521 atttggaaga gcatcattgt atcaatatat ctgtgtagct aatacatctt atgggaaaaa
    14581 ttttttagcc aaatatttgt ggaatgaaaa tccagatgag aatataattt tagaaagaca
    14641 gagagctata aaagaacttt tatcaaaaga tgatttttct attcatattc aaacactcag
    14701 taatataatt ggtaaagagc aaaaaaacaa ttcagatagt agtatagaat cgtttataga
    14761 atatggagaa aataagaatg tgtatatacc aaaatggatg catattttta cttggggttt
    14821 accaactgca acaatcttat cttttatatt ttgtatgtta ggatttttac ctgttttacc
    14881 tgttttttta ctttttataa tccagttagg gtttagtggt tttggatatc caagacttat
    14941 gcaaactctt gctccattat tttcatttag tagaagtata caagtatatg aaaaaatgtt
    15001 tgaggtttta gaaaaagaga cgtttgagag tgcatatttg aaagaattac aagataaact
    15061 ttctaaggga agtggagttt caagaggtgt taaacaatta aattcaatag gaaatgctgt
    15121 aaacctaagg tataatcaga tactatatat aattgcctgt ggtgttttga tgtggaacta
    15181 tcattgtgca gaagctttgg agcgatggaa aggtatatat ggaaatcaaa ttagagattg
    15241 gtttgaatct attggagaat ttgaagctct aataagttta acagttgttt ctcatgtaaa
    15301 ggaaaataca tgttttccag taataaaata tgaagatact ccaagattga aagttgaaga
    15361 ggtataccac ccattaatag cagaaaaaag tgtcatagca aattcgatac aattaaattc
    15421 tcagacttgt attatcacag gttcaaatat gtctggaaag acaacatttt taagaagtat
    15481 cggtgtaaac ttagtgctgg catatgcagg tgctccagta tgcgctaaga gttttgatgc
    15541 gacctgtatg gctatattta cttctatgag aatacaagat gatgtaagtc aaggaatatc
    15601 aactttctat gctgagattc ttagaataaa atcaatgatt caatatagtt taaaagaact
    15661 gccaatgttg gttctagtag atgaaatttt taagggaact aactctgctg atagaattat
    15721 tggagcaagt gaggcagtaa agaaattatc aaaaccctgg gttataagta tggtgacaac
    15781 acatgacttt gagctatgtg acctttcagg tagcggagat gttgaagttg taaactatca
    15841 tttctcagag tactatgtag acgataaaat tcattttgac tataagataa aagatggaag
    15901 atgtaaaact acaaatgcaa aacaacttat gagaatggca ggtattcttt aattaataga
    15961 taggttaatt aataaataag tctatttaga aatataattt aatttgtgaa attaataagg
    16021 attgtcttaa attacgtaaa agtattttaa gacaatcctt ttttaagaat aaattttata
    16081 ctttgttatg actacttagc ttcttcacaa gcacattctt gtggacactc tccatagaca
    16141 caactcttat caaagtaatg agtatgaagg tatttatgag ctacatcact atttggttct
    16201 ccaaagaagt ctttataaat agcttgtaaa tcaggattct catgagattt acgaatagcc
    16261 ttatttctat ctatttcata taacgcattt gcacgtctat caactatttc agtattacca
    16321 tggtgataag gttgaccagc acctccaaca catccaccag gacatgccat aacttctatg
    16381 atatggtatt tacattctcc acttctgacc ttatccataa tctttctagc gtttttaagt
    16441 gtatttacaa tacatacatt aactgtagtt ccatcaacat ctatacttgc ttccctaatt
    16501 cctttaagac ctctaacatt agtgaagttt acattatcta aagtcttatt agttatcttc
    16561 tcatatgaag tacgtagggc agcttctaaa actccaccac ttgctccaaa tatagatgca
    16621 gcacctgtag attttcctag aggattatca aaatcttggt cttgaagatt aggtaaatcg
    16681 attgcagctt cttttatcat cttagctaac tctctagtag ttatagaaag gtcaacatct
    16741 agtattccag attcacttaa ttcttctctt gaagcttcat attttttagc tacacaaggc
    16801 attacagata caacataaag ttcttcagga ttgattccta atattttagg tgcatagtag
    16861 tttttagcta tagaaccaaa cataccttga ggagacttac agctagatgc taagtttaac
    16921 ttgtctggat aattgtgctc taagaagttt acccatgcag gacaacaact tgttaatata
    16981 ggaagatttt cacctttttg gattctttct ataaattcag tagcttcttc cattatagta
    17041 aaatctgcac caaagtttgt atcaaataca tgttcaaatc caagtgcttt taaagcagct
    17101 accattttcc ctgtagagat tgaccctggt tctaatccaa actcttcacc aatagcaact
    17161 cttacagcag gagcaacttg cactactata gtcttttctt ttttatttaa aacatcccaa
    17221 agtttaggta cattatctac ttctgttaat gctccagttg gacagacaga tatacattga
    17281 ccacagaatg tacactctgt ttcaaccata tcagagttaa agaatgtact aactaaagta
    17341 ttaaatccac gattaactcc agataatgct cctactgttt gtatgtcatt acatacagtc
    17401 tcacatcttc tacataatat acacttagaa tggtctttaa ctaaagattt agtagaagta
    17461 tctttacctg caaaagattt agaaccttga tatcttattt ttcttactcc taaatcagca
    17521 gctatagtct gtaattcaca atctccattt ttttcacaga caaaacaatc ttgtggatgg
    17581 tctgataaca ataattcaac aatagttcta cgagcattta atgctttagc actattagtc
    17641 tgaactttca taccttcctt tataactgtt ccacaagctg gaactaatcc tctctctgtc
    17701 tcaaccatac aaacacgaca agaagcacaa gtgtcaagct tatctatttc attcatatgt
    17761 aaatgacaaa ggttgtgtat ttttatattt attaatttcg cagcatctaa gatagaagtt
    17821 ccactaggtg ctgaaacgtg tttgccatta atagttaaat taactaaact cattgttatt
    17881 cctcctccaa ataagttatt tcttgattat agcgttaaat gtacaattat ctatacaagc
    17941 accacatttt agacacttag aagtatctat tgtatgttgt tccttaacag aacctgttat
    18001 acaaccagca ggacacactc tagaacattt tgtacaaccc ttacagtcat ctgttataaa
    18061 gtaagaaagt aagttctgac atttaccagc aggacatttc ttatctacaa catgagctat
    18121 atactcatct ttgaaatatt gaagagtact taaaacaggg tttggagcag ctttaccaag
    18181 accacataat gaagcagttt gtatagtttc agcaaggttt tctaggtcat ctaaatcttg
    18241 tagagtacca ttaccttctg taattttagt taatatttct aataatcttg tagtacctat
    18301 acgacaaggt gtacatttac cacaagattc ttcaacagta aactcaagga agaacttggc
    18361 tatatctacc atacagtcag attcatcaag aacaagcata ccaccagagc ccatcattga
    18421 accgatagaa cttaatgaac caaagtctat aggagtgtct aaatgttcag ttggtataca
    18481 tccaccagat ggtccacctg tttgaacagc cttaaatttc ttaccttcag ggattccccc
    18541 accaatttca aatacaatat ctcttaaagt agtacccatt ggaacttcaa caagtcctac
    18601 attttctacc tttccaccaa gagcaaagac tttagtacca ctagaatctt cagtacctaa
    18661 gttggcaaac caatctccac ccttaagtat aattgcaggt atgttagcaa aagtctcaac
    18721 gttatttaag caagtaggag atttccatag accactctta gaagaactat aagttttcat
    18781 tctaggttca ccacgtctac cttcaattga atgcattagc gctgtacctt caccacaaac
    18841 gaaagcacct gcaccatatt ttaattctaa tttaaagtta aatccagtgc ctaagatatt
    18901 ttcgccaagt aaaccgtatt tttcagcttg agctatggct acttttaatc tttctataga
    18961 ttttggatat tctgctctta tgtatatgta tcctgtatca gaacctatag cgtatccaca
    19021 tattgccata gcttctagta cactatgagg gtcaccttca agtacagatc tatccataaa
    19081 tgcacctggg tcaccttcat cagcattaca tacaacaaac tttttatctg ttggaccagc
    19141 tggatttttt gaagcagctt cccatttaga gccagtaggg aaacctgcac cacctctacc
    19201 acgaaggcca gaagttttta cctctgcaat aacttcttga ggagtaagag aagttaaaca
    19261 tttgcctaga gctaaataac catcattggc aatatattct tctatactat ctgggtctat
    19321 aagaccacaa ttttttaaag ctatacgtaa ctgttttttg tagaaagaca tttcttcttg
    19381 ttcttcaact tttttactta aagattcttc ctcatataat aattcctcga caactgtatt
    19441 tctaattaag tggctttgaa ctatcttttc agcatcagat ggctcaactt ttacgtagaa
    19501 aacgttatca ggataaactt ttacaatagg accttgagca cagaaaccaa aacaacctgt
    19561 caaacgaaca ctaactttat cttggattcc tgatttcttt atttcatttt ctaattcact
    19621 aactatttct agactgtttg aagatgtaca tccagtatca caacaaacaa gtaattctct
    19681 tcttaataca ttttccttag aataattttt tcttaagcta agattaggtt tcagctcatc
    19741 agctaaaact tttaattcat caaatgaatt tactttacgc attatttatt cacccctctt
    19801 aacaattcaa ttctaattct ctataagtat ctaatatttc gcttacttgg tcaggtttca
    19861 ctttaccata aactttacca tttacagtaa caactggagc taaaccacat gcccccaaac
    19921 atcttaaacc ttctaaagaa aatttaaaat cactagtagt ttctccaact tttatgccaa
    19981 gctgtttttc aaaagcactt aagattttgt cagcaccttt tacaaaacat acagttccta
    20041 gacagacact gattttatac ttacctacag gttctgtagt aaaataagaa tagaatgtaa
    20101 caaccccata tacctttgca ggagctactc caagttttct agcaacatga agttgaactt
    20161 cttttggaag atatccaaat ataccttggg cttcatgtag aacttgtatt aacgcacctt
    20221 cctttgttgc aagagagtca ataaatatat ctagttcgtc aaataattgc ttgttgtgtg
    20281 aaataaaatc gcacataaca aatccccctt ttctttttaa agttattatt tttcataaaa
    20341 taattaacca tgagttgacc ccaacattat gtgggtgaac aaaaccaaaa caataaagaa
    20401 agaataatat acaacaattt ctttatttat aaaaacaatg caaaaaagtt acctattaaa
    20461 aataaaaact atactagtct attataatat aaaataattc agaatagtta aaaaaaataa
    20521 aaaaaatgag ctaaattcaa taaaaaaaaa ataatacata taaaaaactt taaaaaataa
    20581 accaaaatgt aaaattttta taaaataaaa gatttataaa actgctgata atagtcttga
    20641 aattgattcc ttgattttta tataagtaga cctagaatta tatatatcta atgttatttc
    20701 cttagaattt aaaatatcat tttcaaatat aattctttgt tccaatgctt tttttgaaga
    20761 gtacataaat gcattaactt cgaaattaag ctcaaaactt ctgatatcca tattagctgt
    20821 tcctatagag catatagagt catctataac tattgtctta gcatgtaaaa atgcattttg
    20881 accataagta tagattttag ctccaaactt taaaagttct ccagcatatg aatatgatgc
    20941 ccagtataca aagggatggt caggtttaga aggaatcatc actctcacat caacaccaga
    21001 taaacaggct atttttaaag tatctataaa agttgagtca agtattaaat aaggactctg
    21061 tatgtatata tactttctag ctttctgaat cattttaagg taaccatatt taatctcatc
    21121 aagttctgat atatctggac cacttgatac tatctgaatc ccaatatttt cagaaggatg
    21181 attagaattt gtatcttctt ctataaagta tttttctaaa tctaagtctt ctttagttgt
    21241 atatctccaa tcaaggatga atctcatatt taggtcaatt acgcaatcac cagtaagctt
    21301 agtatgtgta tctctccata gaccaaattt tggatttcta cctaaatatt catcaccaac
    21361 attatatcca cctacaaatc ctaccttacc atcaatgaca acaatttttc ggtgatttct
    21421 atagtttaag ttaaagttta caattttcaa gaatgatggg aagaagcttc caactttaac
    21481 accactttct ttaagtctgg aaagtgtact attttttagt gttctaccac caactgcatc
    21541 aaataaaagt cttacttcaa caccttcttt agctttgtct ataagaatgt ctattatttc
    21601 agttccgatt ttgtcatctt tgaaaatgta aaattgtatg tttatatatt ttttagcttt
    21661 tttaagttct tcaagaagac tgttaaaaaa ttgactagat tcagcataaa tccacacatc
    21721 gttattattg gtataatgag cattatttga attagccaat gcataaatca tatccttatg
    21781 ttgatgtata tctgaatcaa tttcagatgt agattggagt ttaacctgag tatctagtat
    21841 attacttttg attatcttat catccttttc tttcaatcta aacatattgt tttttgaaat
    21901 atttctgcca aaagctatat aaagtatgaa tccaagagca ggaatcaata ctaatataag
    21961 aagccatgtt aaagttgtat ttatgtttct tttttctcta aagataaggt ttagtataac
    22021 catgaaatta attatatata ttgtagtaac tgctatttca tatattgaca tttcgaaaaa
    22081 tgatatatca aacataaaat tccctccttc aaatatttat taaaataagt aattatatat
    22141 aataacacaa aca
//
LOCUS       DAMJVK010000065        22136 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.066, whole genome shotgun sequence.
ACCESSION   DAMJVK010000065 DAMJVK010000000
VERSION     DAMJVK010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22136
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.066"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            41..376
                     /locus_tag="PC414_002501"
     CDS             41..376
                     /locus_tag="PC414_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2464889.1"
                     /translation="MYNIDDMIYNCPVEALSNILGKKWVAIIIWKIQEDKKRFGELQR
                     AIPDCSKKMLVQQLDFLIEQGIIVNQKKLVNNVVESTYFLSESGLRLLPVMEKMIMWS
                     NKNLNCDKY"
     gene            513..1790
                     /locus_tag="PC414_002502"
     CDS             513..1790
                     /locus_tag="PC414_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HDF2464890.1"
                     /translation="MQNVNLFLVFIEGIVSFFSPCILPILPIYLSILSNSSVENLKEG
                     KTSFIGSSLFKNTIFFALGISTTFFILGSSVKVLSMFFNENKDLIMFIGGIIIIIMGL
                     FYMGIIKSSILNREKRFNVKFKEMKAITAFILGFTFSFGWTPCIGPILASVLVMVSSS
                     SNHLSANLLIAVYTIGFILPFIITAMFYSKLFKTIDKIKSNMEIIKKIGGIILIVSGI
                     LMMVNGFGSISKHFNTSQNSKIESKQEENKRENSTDKEENSDGNDSQKDSNNDNNDKG
                     SNDEDRIKSIDFTLTDQYGKTHKLSDYEGKVVFLNFWATWCPPCKEEMPYIEQLYKDY
                     NKNNDDVVILGVASPNLGREGSREHVVNFLKDQGYTFPVVLDEDGALAYQYGINAFPT
                     TFIIDKEGYVTQYIPGAMDKATMASFIENQRNK"
     gene            1911..3299
                     /locus_tag="PC414_002503"
     CDS             1911..3299
                     /locus_tag="PC414_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDF2464891.1"
                     /translation="MKKTFDAIIIGFGKGGKTLAGDLANRGLKVALIEKSNKMYGGTC
                     VNVACIPTKSLENSANSVKTKNINSWDEVQAEYEKAIDKKETLITKLREANYNKLNSN
                     ENVTIFTGMGTFIDEKTVQVKTENEIYELVADNIFINTGSRPFIPNIKGIENKNIVYD
                     SESLMNLRTLPKKMTIIGAGFIGLEFAGIYSSFGAEVTILNSNNGILPNEDVEDSEEI
                     IKLLAKRNVKIVNNANIKEIKEVSELAIVEYEVDGKSKELTSNMILVATGRKANTEEL
                     GLENAGIELNERGFIKVSETLKTNKEHIWAIGDINGGPQFTYISLDDYRIVINQLFGD
                     KTRTTNDRKNIPNSIFISPAFSRVGLNVKQAKEKGYEVLVAKMPVEAIPRAKQIGKAD
                     GFIKIVIDKKSNKILGASMICENSSEIIHLIQLAVDLEVEYTYLRDRVYAHPTMTEAL
                     NDILSPNMIKEV"
     gene            3670..4383
                     /locus_tag="PC414_002504"
     CDS             3670..4383
                     /locus_tag="PC414_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017208807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF434 domain-containing protein"
                     /protein_id="HDF2464892.1"
                     /translation="MNKISKRGFDDSDKRWFSSKELTRLIKSKEEIEWLLNRDYKIDP
                     VVTFVGDRYQFSIRQRDALKRAVCTDEKNIIRQSKRLSLDKIKEDTVNIDGFNLIISI
                     EVALSGGTLVIGSDGNIRDLAGLRGTYKIIDKTEEALNLIGKFFNKYNAQNLKFYLDA
                     PVSNSGNLKYRILEHAKTWGIETEVELVKNADVVLEKLDRVVSSDAVIVDKCISYFNV
                     ARGIIEEYIKECNIINLNK"
     gene            4501..5370
                     /locus_tag="PC414_002505"
     CDS             4501..5370
                     /locus_tag="PC414_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDF2464893.1"
                     /translation="MRGWICVKLTKLNIKKYRAPIYKYALANVNLRSAKSTNSSIITV
                     IPQGAKMEVLDEEDDWIKVMYNSQEGYVYKDLVSVSEYAWSNLNLREDKSTTSNIITV
                     IPEKSRVEVLQVDGDWSKVVYDDKIGYVFNYFLSIDGNKPNELDYKYFYTDMIKFVNE
                     NNIKSTSDYLIVTDLRNKYTYIFKKDNGGWGQLYKWQCTIGKPETPTITGIFYISGRK
                     PSFGTDEYSVKYATRIKGGYYYHSVLYDSTGSYIIDGRLGEALSHGCIRLSTENAKWI
                     YDNIPDTTTVIIH"
     gene            complement(5525..6670)
                     /locus_tag="PC414_002506"
     CDS             complement(5525..6670)
                     /locus_tag="PC414_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HDF2464894.1"
                     /translation="MSIYYVPPINLLGKGCLNEAKDSIKSLGTKKAFIVSDKFLVSNG
                     TVKKVTDILSQIDVDFVVYDEVKQNPTVTNVNKGLKILKSENCDFVITIGGGSPQDCG
                     KAISILATNGGDIRDYEGINKTSKKCLPIIAITTTAGTSAEVTINYVITDEDRHVKMI
                     MVDTNSLATMTVNDPELMISKPSNLTAATGMDALTHAIEAVVANGAYDVTDSTALYAI
                     KQIFKYLPRAVKNGNDIEAREQMCYACFLNGIAFSNAGLGNVHAMAHQLGGLYDLPHG
                     VCNAMLLPIVEEENAKSAPAKFRPIAEVIGMDVNNKSDKECVDFVIDKIKALSEEVGI
                     PKSLKDVGVDNPNFELLAENSMKDACAGANPVFFDKDKIIELFMKIS"
     gene            complement(7003..7779)
                     /locus_tag="PC414_002507"
     CDS             complement(7003..7779)
                     /locus_tag="PC414_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDF2464895.1"
                     /translation="MTSDNHRPTARILDILEALAESENGYTLTEIADVINAPKSSIFP
                     IVHTLNARKYITIDKNTSKYSIGIRSYTLGSSFFEKRTIFNYIIDEMTNIVNSCSETC
                     QLGILDNNEVLYIGKVDSPEPIRLISYVGKKIPANCTGLGKALLCDFDKEKLISLYPD
                     GLKGFTEKSITDFDVLYDQLLEVQRTQIASECEESMEHLKCLAVPIRKNGCIRAAVSV
                     TLPLFRANDEKIELIKKLLLNAQKNIQSMMEERNEDIVIN"
     gene            8361..9335
                     /locus_tag="PC414_002508"
     CDS             8361..9335
                     /locus_tag="PC414_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HDF2464896.1"
                     /translation="MIMDFIKKIPAGMMIVPMFIAAFINTFCPDIVQIGSFTTAVFSS
                     VGSATIIGVQLVCLGTQLQFREMPKVLKRGGILLGSKFAIGALIGILIGKLFGMDGVL
                     GLTTLAVISAVTNSNGSIYLSLMTSYGDETDCATMSLLTLNDGPFFTLIALGTSGLAN
                     VPLLSLLAAIVPILVGMIIGNFDKKMKEFLEPGCNLLVPFVGFALGSSINLASILKGG
                     LSGVLLGLISVFVGGIFIVFCDKFIGKRPGYAGWAVATTAGNAIAVPAAVALVDPSWQ
                     PYVATATTQVAAAVVVTAILVPLITSWWAKKYGCPKFPKQNEDGTITA"
     gene            9433..9738
                     /locus_tag="PC414_002509"
     CDS             9433..9738
                     /locus_tag="PC414_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HDF2464897.1"
                     /translation="MINAVIIDEKDNVAVAIEAIAKGSEISFKLKDKTVKTITALDDI
                     TIYHKLAIKDMAQGDKVTKYGEHIGEANQEIKSGQHVHIHNVRSVREDLDKQVMTNI"
     gene            9894..11054
                     /locus_tag="PC414_002510"
     CDS             9894..11054
                     /locus_tag="PC414_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HDF2464898.1"
                     /translation="MKFYGYKRPDGRVGIRNHILILPTSVCASDTTRIIASQVQGAVT
                     FNNQNGCSQVHSDQQLTMDVMAGYAANPNVYGVIVVSLGCENCQNDLVVDAIKERTNK
                     PIKTLVIQEEHGTLKTIEKAVRYAREMAQEASLLRKEEFPISELILGTECGGSDPTSG
                     LAANPLIGELSDKLVDLGATSILSETTEFIGAEHILARRAVNEEVKEKILHIVHRYEN
                     SLKLVGEEVREGNPSPGNIAGGLTSLEEKSLGCIHKGGHRQISEVYDYAKQIDKKGLV
                     IMDTPGNDASSVAGMVAGGAQIVVFSTGRGTPSGNPIAPVIKITGNKITFANMEDNLD
                     FDASPVIYGPQTMEELTDDLLNMVVDVANGKQSKAESLGYTEMAIARVCNYV"
     gene            11104..12006
                     /locus_tag="PC414_002511"
     CDS             11104..12006
                     /locus_tag="PC414_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HDF2464899.1"
                     /translation="MKFVSFTEGENECKRTGVFSKDELFIIDVNSLNLSRNFKDLNEL
                     IQNVSSEDIIKLKTIINESTFYNYDVFKRERCTVHVPIEKPIHDILCVGVNYKDHLEE
                     TQTHFDDSFEEPQKTVYFTKRVTKAIGPEDEIDGHFEINNQLDYEVELGVVIGKTGVN
                     IRKEEVEDYIFGYTVINDISARKLQKEHVQWFRGKGLDTFTSLGPCILHKSAVPFPIK
                     LKVCSRVNGEERQSSNTGLFISDVSDIVSELSKGMTLEAGDIIATGTPSGVGMGFSPP
                     RYMSSGDVVECEIESIGILKNYIK"
     gene            12086..12979
                     /locus_tag="PC414_002512"
     CDS             12086..12979
                     /locus_tag="PC414_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HDF2464900.1"
                     /translation="MKAEFLTPVVTIFDEEGNLDKEGNKKVYDHLIDGGVDGLVIMGS
                     TGEFFNMSMDMQKELIDLVTSHVNKRVKVFIGTSRMCINESIELSNYAHNSGADGVMI
                     ISPYYFSLDDDSIELFYSEIAKNTEAEIYLYNYPDRTGYDLSPELTLRLARKHKNIVG
                     YKDTVTLMGHTRDLINTMKIEFPEFKVYSGYDENFIHNLMSGGAGCIGGLSNLIPEVC
                     SKWAEAYNNRDMEKVIEIQKYINKAMDFYLITNPFIPAMKKAMNIRGLQISDYMTAPF
                     VKPNEKQVCEIKKLMNELNIC"
     regulatory      13032..13213
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            13441..14571
                     /locus_tag="PC414_002513"
     CDS             13441..14571
                     /locus_tag="PC414_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2464901.1"
                     /translation="MKFKSNVLKQFFLIAMVIFMVGGLIGCSKSDKENKQASTGKKLE
                     LKYASGFSVEDLEDGIKKVTDGDNRELILIPKKIKIPEKYKNANIVRTPVDNVLIAST
                     TQACSLRTIDELDSIKAVTTQEQYWTIKEIKDLMKDGKIHYVGSNLAPDYEKIVDLNP
                     DLTIVSSGLSEADTKFIDKLKELGLNYVVDNEYKEQNPFGRMEWTSLIAAFYDKEDMA
                     TSELNKTVQKVEEVSKKIKNKAKPKVVWASVYEGSAYIAPKDSYVDNMIKMAGGINAC
                     ASIDSNEGRVSIEQLHEVAKDADIFVYSSSSDYAPNLDSIKSSAPVLADLDVVKNGNL
                     WVFAPDYYQSVDKTDELIVDLVEMFHPGTTGEKIKHYINYDK"
     gene            14643..15668
                     /locus_tag="PC414_002514"
     CDS             14643..15668
                     /locus_tag="PC414_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDF2464902.1"
                     /translation="MDFINKMRIRNTTIFIVLIISMILTFLVCVAFGSVKIEFKEVID
                     VVLRGMDNTEHYKIIYNIRIPRVLATMVGGACLAVSGILLQIFFKNPIVEPYILGVSS
                     GSTLSVALMILGGYTLGFESVNSMSIFVAAFIGALIVMSIVILFANKVKNITTLLIIG
                     IMTGYVCSGITNVLQSFANSDKLKEFTMWSMGSFSGFTWEKVEILIIVGILGLLMTVF
                     IIKPLNAFLLGEEYAKSIGINIRFFRVLIVFISSILAAIVSAFAGPIGFIGLAVPQIS
                     KMIFKTSDNRVLIPVVIFLGSIVTALCDLISRVILSPVELPISTITSLLGAPIVVMLL
                     VKRKDRA"
     gene            15671..16867
                     /locus_tag="PC414_002515"
     CDS             15671..16867
                     /locus_tag="PC414_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2464903.1"
                     /translation="MLKTNNLSVGYNNKVVISNINVEVKNGEILCLLGSNGAGKTTLL
                     RSLSKLISPIKGEIYLNGVNINCISRKALSKKMALVLTNRLLGDLMTVQDIVNIGRYP
                     YTGFFGSLSKKDLIMVDEALESVDALHLKKRYFDELSDGEKQKVLVARALVQEPEIII
                     LDEPTTHLDIKHRLELINILKKLSKEKSISVILSLHEIDIALKSCDKVALIKNNKVIA
                     YGQPEDVVDEDIINSLYELDDKNFNSLLGSVEISNKSKNEVFIIGGGGKATPIYRAFT
                     KKGIGLYSGIIHENDIDYEIGRTMGIKMFTENPFEPISDESFDLAIRNLNDSKIIIDT
                     GFSVGETNKRNIDIIKEALKLDKKVYSFRNRDESKKYYDSLDSKIEHIDKVSQIINSA
                     DINNLL"
     gene            complement(16995..17720)
                     /locus_tag="PC414_002516"
     CDS             complement(16995..17720)
                     /locus_tag="PC414_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDF2464904.1"
                     /translation="MKTISKQELEKTLEKHYLWLKNNNAGEKADLKELRLENMDLRGY
                     CLSNIDFSWSDLINLNLEKADLENSIFNNSYLSDCSLKNSILKNADLSGASFRFCDLS
                     NSDIRGANLENSNLEYATLNGVISDESTRFFKLYCPETGAFIGYKKCFNFRMVQLLIP
                     SDAKRVSATSNACRCDKAKVLSITSVDGKESFKSARAYADENFIYRVGETIVAEDFNE
                     NRWKESTTGIHFFLTREEAIGYL"
     gene            18198..20297
                     /gene="nrdE"
                     /locus_tag="PC414_002517"
     CDS             18198..20297
                     /gene="nrdE"
                     /locus_tag="PC414_002517"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HDF2464905.1"
                     /translation="MNWIELNNQVIIKNEQGKYQLEKDKEALSSYIEEFIQPKLRKFN
                     NLEERLKYLIDEGYYSKEVINQYSIDVIKNIYDMIDKHKFEFQSYMSANKFYQNYALK
                     SNDGKEILETYNDKVLIVALTLGNGDKDLALNLADKIVKQEFQPATPTFLNAGRKRAG
                     EMVSCFLLSVEDSTEGISYAISSSNHLSKIGGGVALNLSKLRASGESIKDIEGAAGGV
                     VGVAKMLEQSFSYFNQMGARQGSGAVYLTVFHPDFELLMDTKKINADEKIRLATLSLG
                     AIIPDKFMELAEKNEVAYAFYPHTVYKKYGVSLDEIEMDKWYDKLVNDSDIRKKEINP
                     RQMLTKIAQMQQESGYPYVVYIDTANREHTLKDVGIIKMSNLCCEIFQYQTPSEIEGY
                     GGKNEWGQDISCNLGSLNIANVMDNKTIESTVETAIRALSFVADSTDIKPVPTVSNSN
                     SKSHSIGLGAMNLHGYLVRENILYTSDDAIDFSNVFFAMVRYYSIKASMKIAIERNQT
                     FEGFDKSEYVKGRNSKVLSKYYEQSYLPKSEKVRALFEGIYIPTKEDWTKLLDEVKEK
                     GIYNAYLMAVAPTQSISYVQNATSSIMPITEPVEVRTYGDSTTIYPMPFLTNDNMLYY
                     QSAYRMDMRKVIDLVATVQNHVDQGISTTLFVTDEKTTRDIARHYIYAYKKGLKSLYY
                     TRTKMTRDTHECLVCSV"
     gene            20310..21281
                     /gene="nrdF"
                     /locus_tag="PC414_002518"
     CDS             20310..21281
                     /gene="nrdF"
                     /locus_tag="PC414_002518"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HDF2464906.1"
                     /translation="MTFNLHKIHNAVNWNREEDGFTQAFWEQNVKQFWLPEEISVSKD
                     IKVWNELSNKEKELYKKVLGGLTLLDTKQGNNGIPSMMSLTENLQRKAVLSFMGTMEE
                     IHAKSYSSIFMTLLSNLEIDELFEWIETEPTLQRKADLVLAQYENTTNQEGLYLSMVT
                     SVFLESFLFYSGFFYPLYLSGQGKMVASGEIISLILRDESLHGKYIGLLAQEIYDSFD
                     KMDKEMLEEKMYSILYSLMENEIEYTNVIYRESGLEKEVVNFLKYNANRALENLGFEK
                     LYTVDAINPIVLNGLSTETKTHDFFSTKGNGYQKGVYEELEDEDFII"
     gene            21357..21983
                     /locus_tag="PC414_002519"
     CDS             21357..21983
                     /locus_tag="PC414_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HDF2464907.1"
                     /translation="MKYEWRKRDKELYLPKKNPVIVEIPEQKFIMLKGSGDPNSKEFT
                     EAIGVLYSLAYAIKTMPKKGIVPEGYFDYTVFPLEGIWDKGEKSKGLDVLIKEDLIYT
                     IMIRQPDFVTQDLLDKATDIVKKKKPHNLLEKVRFGSINEGLCVQMLHIGSYDNEPES
                     FDIMNEFCKKNKLIRKSYTHREIYISDARKVSPESLKTVLRFEVEREV"
BASE COUNT         8576 a         2448 c         3881 g         7231 t
ORIGIN      
        1 gtttgtttaa taaattaaat aaagaaaaga ggagaataaa atgtacaaca tagatgatat
       61 gatatataat tgtccagtag aagctttatc aaatatttta ggtaaaaaat gggtggctat
      121 aattatatgg aaaatacaag aagataaaaa acgatttgga gagctacaaa gagctatacc
      181 agactgtagt aagaaaatgt tagttcaaca actagatttt ttaattgaac aggggattat
      241 agtcaaccaa aaaaagttgg ttaataatgt tgtagagtca acatattttt taagtgaatc
      301 aggattacga ttgcttcctg tcatggaaaa aatgattatg tggagcaata aaaatttaaa
      361 ttgtgataaa tactaaaatt tactctgtaa gtaatgaaaa tttaattaag ctatgagtag
      421 aaaaatgtat taaaattaca taatttttta gttagatttt aatttagaaa tgggattgta
      481 atattggaaa taaaataagg aggaatttta atttgcaaaa tgtaaattta tttttagtat
      541 ttatagaagg tatagtatca ttcttttcac catgtatact gccaattttg cctatttatt
      601 tgagtatatt gtcaaatagt agtgtagaaa atttaaaaga aggaaagacg agttttatag
      661 gaagttcttt atttaaaaat actatatttt ttgctcttgg aatctcaaca acattcttta
      721 tattgggttc atcagtaaaa gtattgagta tgttttttaa tgaaaataaa gatttaataa
      781 tgtttattgg tgggattata attattatca tgggattatt ttacatggga ataattaaat
      841 cttcaatatt gaatagagaa aaaagattta atgttaagtt taaagaaatg aaagcaataa
      901 cagcttttat attaggtttt acatttagtt ttggatggac accttgtatt ggtccaatat
      961 tggcatctgt attagtcatg gtttcaagtt caagtaatca tcttagtgcg aatcttttaa
     1021 tagcagtata tactattggt tttattttgc cattcataat tacagctatg ttttacagta
     1081 aactatttaa aactatagat aagataaaat ctaacatgga aattattaaa aaaataggtg
     1141 ggattatact tatagtatca ggaatattaa tgatggtaaa tggatttgga agtataagca
     1201 aacactttaa tacgtcacaa aatagtaaaa ttgaaagcaa gcaagaagaa aataagcgag
     1261 aaaattctac tgataaagaa gaaaattctg atggtaatga tagtcaaaaa gactctaata
     1321 atgacaataa tgataaaggg tctaatgatg aagatagaat aaagtctatt gattttactt
     1381 taacagacca gtatggtaaa acacataaat taagtgatta tgaaggcaaa gttgtattct
     1441 taaatttttg ggctacatgg tgtcctccat gtaaagaaga aatgccttat atagaacaac
     1501 tgtataaaga ctataataaa aataatgatg atgtagtaat tttaggagta gcttcaccta
     1561 atttaggtag agaaggttca agggaacatg tagttaactt cttaaaagac caaggttata
     1621 cattccctgt tgtacttgat gaagatggag cattagcata tcaatatgga attaatgctt
     1681 ttccaactac atttataatt gacaaggaag gatatgtaac tcagtatatt ccaggagcta
     1741 tggataaggc aactatggca tcatttattg aaaatcagag aaataaataa aagtatttaa
     1801 gtataagttt ataaatcaat ttaaggttac aaaaaagtgc ctaattgtac cacctaaata
     1861 atcttgttat agtaaaagta taaacaatac aagaaagggt tgaattttat atgaaaaaaa
     1921 catttgatgc tataataata ggatttggaa aaggtggaaa aacattagca ggggatttgg
     1981 caaacagggg attaaaagta gctttaatag aaaagtcaaa caagatgtat ggtggaacat
     2041 gtgtaaatgt ggcatgtata ccaacaaaaa gcttagaaaa ttcagcaaac agcgtaaaaa
     2101 caaagaatat aaattcttgg gatgaagtac aagctgaata tgagaaagct atagataaaa
     2161 aagaaacttt aattacaaaa ctcagagaag ctaactataa taaattaaat tcaaatgaga
     2221 atgtaactat attcacagga atgggaactt tcattgatga aaaaacagtt caagtcaaaa
     2281 ctgaaaatga aatttatgag ttagtagcag ataatatatt tattaatact ggttcaagac
     2341 catttatacc taatattaag ggaattgaaa ataagaatat agtatatgat agtgagtctc
     2401 ttatgaatct aagaacttta cctaaaaaaa tgacaattat aggagctgga tttataggtt
     2461 tagaatttgc tggtatttat agttcatttg gagcagaagt tacaatatta aactctaata
     2521 atggcatttt accaaatgaa gatgttgagg attcagaaga aataataaaa ttacttgcaa
     2581 agagaaatgt aaaaattgtt aataatgcaa atataaaaga gattaaagaa gtttctgaat
     2641 tagcaatagt agaatatgaa gtagatggta aatctaaaga attaacaagt aatatgatac
     2701 ttgtagctac aggaagaaaa gcaaatactg aggagctagg gctagaaaat gcaggtatag
     2761 aattaaatga aagaggattt attaaagtct cagaaactct taagactaat aaagagcata
     2821 tatgggctat aggagatata aatggaggtc cacaatttac ttatatctca ttggatgatt
     2881 atcgcatagt tattaatcag ttgtttggag ataagactag aacaactaat gatagaaaga
     2941 atatcccaaa ttctatattt ataagtccag cattttcaag agttggtctt aatgttaaac
     3001 aagctaaaga aaagggatat gaagtattag tggcaaagat gccagtagaa gcaattccaa
     3061 gagctaaaca gattggcaag gcagatgggt ttataaaaat agtgatagat aaaaaatcca
     3121 ataagatact tggagctagt atgatatgtg aaaattcaag tgagattatc catcttattc
     3181 aattagctgt agatttagaa gtagagtata catatttaag agaccgtgtt tatgcacatc
     3241 caactatgac agaagctctt aatgatattc tatctcctaa tatgataaaa gaagtataac
     3301 ataagtttaa tatataaacc taaaattatc attataagta gattttagaa agaggctatc
     3361 tcaaaaaata gagataagtc tcttttttac tgtttaaatg ataaaaatat atttgatttt
     3421 ataaaagaag attatcttat gaactaaaaa gtttatttta aaacactacc tatttatgtt
     3481 ataaacttat aaatataata tagaatctat tattctgtat tattaagtgg ggttaagcgt
     3541 tataattaat tataaatatt gaataatcta aatggataag ataatggtat taaaaattat
     3601 cttaaaaggt gaaataatct gtatttaatg tagtacagtt atatctttaa agcaaggtgg
     3661 tgaaatgata taaataagat atctaaaaga gggtttgatg atagtgataa aagatggttt
     3721 tcaagtaaag aattaacaag acttataaag tctaaagaag aaatagaatg gttattaaat
     3781 agggattaca aaatagaccc agtcgttact tttgttggtg atagatatca gttttctata
     3841 agacaaagag atgcattaaa aagggcagtc tgtactgatg aaaaaaatat tataagacaa
     3901 tcaaaaaggc tttctctaga taaaatcaaa gaagataccg taaatataga tggctttaat
     3961 ttgataatat ccatagaggt agcattatct ggtggtactc tagtaatagg aagtgatggc
     4021 aatataagag atttagcagg tcttagggga acctataaga ttatagataa aacagaagaa
     4081 gctttgaatc tgataggtaa attttttaat aaatacaatg cacagaatct taaattttat
     4141 ttagatgctc cagtttctaa ttcaggaaat ttaaagtata ggatattaga gcatgccaaa
     4201 acttggggaa ttgaaactga agtagaatta gtaaaaaatg cagatgttgt attggaaaaa
     4261 ttagatagag tagttagtag tgatgcagtc atagttgaca agtgtataag ttattttaat
     4321 gtggctagag gtataattga agagtatatt aaagaatgta atatcataaa tttaaataaa
     4381 tagataaata taggtgagaa tatataagta tatgtagcaa tattcttagt aatacaagta
     4441 aagtttgaaa ataggagaac gaatacatat cgaatttcat aaaatgttgt aaaaagacat
     4501 atgagaggat ggatatgtgt gaaattaact aaactaaata ttaaaaaata tagagcacct
     4561 atatataaat atgctttagc aaatgtaaat ttgaggtctg ctaaatctac taattcaagt
     4621 atcataacag taatacctca gggagccaaa atggaagtat tggatgaaga agatgactgg
     4681 attaaggtga tgtacaattc tcaagaagga tatgtgtata aggatttggt atcagtaagc
     4741 gaatatgctt ggagtaactt aaacttaaga gaagataaat ctacaacttc caatataatt
     4801 acagtaattc ctgaaaaatc tagggttgaa gtccttcaag tagatggaga ttggagtaag
     4861 gttgtatatg atgataaaat aggatatgtt tttaattatt ttttatcaat tgatgggaat
     4921 aaaccaaatg aattagatta taagtatttt tatactgaca tgattaaatt tgttaacgaa
     4981 aataacatta aaagtactag cgattatcta attgtaacag accttagaaa taaatatact
     5041 tatattttta agaaagataa tggaggatgg ggacaacttt acaagtggca atgtactata
     5101 ggcaagcctg agactccaac tataacaggt atattttata taagtggaag aaaaccttct
     5161 tttggaacgg atgagtattc agttaagtat gctactagaa ttaaaggtgg atattactat
     5221 cattctgtac tatatgattc aacaggaagt tatataatag atggcagact tggtgaagct
     5281 cttagtcatg gatgtataag attaagtacg gaaaatgcaa agtggatata tgataatata
     5341 ccagatacaa caacagttat aattcattaa gaaaaatttt gagtttttta ttgggataat
     5401 aattcttaaa ttaatagaat tatatattaa cttctcataa agagtactat ttaaaaagtg
     5461 taataagaga gtgctattaa caaaacaaag atattttgag gcactctctt ataaatatac
     5521 ataattaaga tattttcata aacaattcta taatcttatc tttatcaaag aaaacaggat
     5581 tagcaccagc acaagcatct ttcatagagt tttctgcaag caattcaaaa ttaggattat
     5641 caacacctac atcttttaaa gattttggga ttccaacctc ttcagaaaga gcttttatct
     5701 tatctattac aaagtcaaca cactctttat ctgatttatt atttacatcc attccaataa
     5761 cttcagctat tggtcggaat ttggcagggg ctgatttagc attttcctcc tcaactattg
     5821 gaagtagcat ggcattacat actccatgag gtaaatcata taaacctcct aattgatgag
     5881 ccatagcatg tacatttcca agacctgcat tactaaaggc aattccattt aaaaaacaag
     5941 cataacacat ttgttctcta gcttctatgt cattaccatt tttaacagct cttggaaggt
     6001 atttaaatat ctgctttata gcatataagg cagttgaatc tgtaacatca taagcaccat
     6061 tagctactac agcttctata gcatgagtta gtgcatccat accagttgct gctgttaaat
     6121 tagatggttt gctaatcatt aattctgggt catttactgt cattgtggct agtgaattgg
     6181 tatcaaccat tatcatcttt acatgtctgt cttcatcagt tataacatag tttattgtaa
     6241 cttcagcaga tgtaccagca gtagtagtta tagctatgat aggaagacat tttttagaag
     6301 ttttgttgat accttcatag tctctaatat ctcctccatt agtggctaat atagagatag
     6361 ctttaccaca gtcttgtgga gaaccacctc ctatagttat aacgaagtcg caattttctg
     6421 attttaatat tttaagtcct ttattcacat tagttacagt tggattttgt ttaacttcat
     6481 cataaacaac aaagtctaca tctatctgag ataaaatatc tgttactttt tttacagtac
     6541 catttgaaac tagaaattta tcactaacaa taaatgcttt tttagttcct aatgatttaa
     6601 ttgaatcttt agcttcatta agacatcctt tcccaagtaa atttattggt ggtacataat
     6661 aaatgctcat aactaaaatc tccttttatt ttcattgatt tttatgtaaa aatatatatc
     6721 taacaatttt cactaactta ctcataatat atcgtatctt agattgagat aggattgttg
     6781 gttcttatat aagaacttaa ttcgtaatta cgattatgat tagaatttaa catagtattt
     6841 ttatcatgtc aataaaaata taaaaaatat tataaaaaaa acaaagttac tgtagttaaa
     6901 tcagcaactt tgtttttata gtataaatca aataaataca ttagtttaaa acaaaatttt
     6961 taattattat gttttgatat cttccatata cagaatatta atctaattta ttacaatgtc
     7021 ttcattacgt tcttccatca ttgattgaat atttttctga gcatttaaga gtaatttttt
     7081 tatcagttct attttttcgt cattagctct aaataaaggc agtgttacac taacagcagc
     7141 tcttatacat ccattttttc ttattggtac ggcaagacat tttaggtgtt ccattgattc
     7201 ctcacactct gaagctattt gtgttctttg aacttcaaga agctggtcgt ataaaacatc
     7261 aaaatctgta attgattttt ctgtaaaacc ttttagacca tctggatata agcttattag
     7321 tttttccttg tcaaaatcac ataaaagagc ttttccaaga cctgtacaat tagcaggtat
     7381 tttttttcca acataagaaa taagtctaat tggttctgga gaatctactt ttccaatata
     7441 aagaacttca ttattgtcaa gtataccaag ctgacaagtt tcagaacagg aattaactat
     7501 attagtcatt tcgtcaatta tatagttaaa aatagttctt ttttcaaaaa atgaagaccc
     7561 taatgtgtaa gaacgtatcc ctatagaata tttggatgtg tttttatcaa tagtaatgta
     7621 ttttctagca ttaagtgtat gaactatagg aaagatgcta ctttttggag cattgataac
     7681 atctgctatt tctgtaagag tatatccatt ttcggattct gctaatgctt ctaaaatatc
     7741 taatatccta gctgttgggc gatgattatc tgaagtcata aatttccccc tctttttatt
     7801 aatttaaagt attagaatat gttgatttta gcacactata cgatatttta caatgtagaa
     7861 aataaatatg taaatttata tataaataag ctcttgattt agatacagat agatattttt
     7921 ttagactgta cattaaggaa taactaattt ccaaaatgta cctaaagaaa gtaagtgata
     7981 tttagttata taatttaagt aaatatatgc aatagttatt tttatatgta aatatcaaaa
     8041 tattatgaac ttatatttta tctatttttt acactgtata aaaactttat agttttgaat
     8101 actatataat tttaaatacg ctgtattttt gcatataagg aagtcatttg ataatataca
     8161 aggtattatc agaaagtttg aaaaaatttt gacaaaaatt tatttttttt attgactcat
     8221 aaataggaaa atgctatcat taattcaaag attcgtaatt aaaaacttag ttcttaaata
     8281 cgaaccgaaa cggccgttag aattaaagct aaaaacttaa taagagtaat taaatatcat
     8341 gttttaggag gattaacttt atgataatgg attttattaa aaaaattcct gcaggtatga
     8401 tgatagtgcc aatgtttata gcagctttca ttaatacctt ttgcccagat attgtacaaa
     8461 taggttcgtt tacgacagca gtattttcaa gtgttgggtc tgcaactata attggggttc
     8521 aactggtatg cttgggaact cagcttcaat ttagagaaat gccaaaagtt ttaaagagag
     8581 gtggtatact tcttggttct aaatttgcta taggtgcttt gataggtata cttataggaa
     8641 agctatttgg aatggatggt gtccttggtc ttacaacatt ggctgttata agtgcagtta
     8701 caaatagtaa tggtagtata tatctttcat tgatgacttc atatggagat gaaacagatt
     8761 gtgcaactat gagtttactt actttaaatg atggaccatt ttttacatta atagctcttg
     8821 ggacttcagg tcttgcaaat gtaccattgt tgtcattatt ggctgcaata gtgccaatac
     8881 ttgttggtat gataatagga aattttgaca aaaaaatgaa agagttttta gagccagggt
     8941 gtaatttact cgtacctttt gtagggtttg cacttggtag tagcataaat cttgcaagta
     9001 tactaaaagg aggtttatca ggagtattac ttggattaat atctgtattt gtaggaggta
     9061 tatttatagt attctgtgat aaatttatag gaaaacgtcc aggatatgca ggatgggctg
     9121 tagctacaac tgctggaaat gcaattgctg tacctgctgc tgttgcatta gttgacccat
     9181 catggcaacc atatgttgca acagctacaa cacaagtggc agccgcagta gttgtcactg
     9241 caatattagt acctcttata actagttggt gggctaagaa gtatggatgt cctaagttcc
     9301 caaaacaaaa tgaggatggt acaataacag cttaatttat attacagtga gttgttggta
     9361 aaataataaa ttctttaatt agattttagg atattaataa aattatatta tttataaagg
     9421 agagcaagaa taatgataaa tgcagtgata attgatgaaa aagataatgt tgcagtagct
     9481 attgaagcaa tagctaaagg tagtgaaatt agttttaaat taaaagacaa aacagtaaaa
     9541 acgattacag cactagatga tataactatt tatcataaat tggctataaa agatatggcc
     9601 caaggtgata aagttactaa atatggtgaa catataggag aagcaaatca ggagataaaa
     9661 agtggtcaac atgtacatat tcataatgtt agaagtgtta gagaggattt ggataaacaa
     9721 gttatgacta atatataaaa taatatttat agagttataa aagttgtatt cattgatttt
     9781 agtgtcaaat agtttttaaa taggttttta gctgtttgat tagtgagtgt aataaaaatt
     9841 ataatatata aaaattatag aaagtatata gactatttta ggaggaaatc agtatgaaat
     9901 tttatggata taaaagacca gatggaagag ttggtataag aaaccacata ttaatattgc
     9961 ctacgagtgt gtgtgcatca gatacaacta gaataatagc atcacaagtt caaggggcag
    10021 ttacctttaa taatcagaat ggatgttcac aagtacattc agaccaacaa ttaactatgg
    10081 atgttatggc tggttatgca gcaaatccaa atgtatatgg tgtaatagtt gtatctttgg
    10141 gatgcgaaaa ttgccaaaat gatttggtag ttgatgcaat aaaagagaga acaaataagc
    10201 caataaaaac tcttgtaata caagaagaac atggaacttt aaaaactata gaaaaagctg
    10261 ttcgttatgc tagagaaatg gcacaagaag cttctttgct tagaaaagaa gaatttccaa
    10321 tatctgaact tatactagga actgaatgtg gtgggtctga cccaacaagt ggattagcag
    10381 caaatccatt aataggtgaa ttaagtgata aacttgtcga tttaggagca acatcaattt
    10441 taagtgaaac tactgaattt ataggtgcag agcatatact agctaggaga gcagtaaatg
    10501 aagaagtaaa agaaaaaata ttacatatag tacatagata tgaaaattca ttaaaattgg
    10561 ttggagaaga agttagagaa ggtaatccat ctcctggaaa tatagctggt ggtttaacaa
    10621 gtttagaaga aaaatcactt ggttgtatac ataaaggtgg tcatagacaa ataagtgaag
    10681 tatatgatta tgccaaacaa attgataaga aaggtcttgt tataatggat acccctggaa
    10741 atgatgcttc atctgttgct ggtatggtag caggaggagc acaaatagtg gtatttagta
    10801 caggaagagg aacaccatca ggaaatccaa tagcaccagt aataaaaata actggaaata
    10861 aaataacttt tgcaaatatg gaagataatc tggattttga tgctagtcca gttatatatg
    10921 gtccacaaac aatggaagaa ttaacagatg atttgttaaa tatggttgta gatgtagcaa
    10981 atggaaaaca atcaaaagca gagtcccttg gatatactga aatggcaata gctagagtat
    11041 gcaactatgt ataatcttta agtaagaaaa taaatacata gaattttaat aaagggtgag
    11101 attatgaagt ttgtaagttt tactgaagga gaaaatgaat gtaaaagaac tggagttttt
    11161 tctaaagatg aattatttat tatagatgtt aactctttaa atctcagtag aaattttaag
    11221 gatttaaatg aattaattca aaatgtatct agtgaagata ttattaagtt gaaaactatt
    11281 ataaatgaaa gtacattcta taattatgat gtttttaaaa gagagagatg tactgtacat
    11341 gttcctatag aaaaaccaat acatgatatc ttatgtgtgg gagtgaacta taaagaccat
    11401 cttgaagaaa ctcaaactca ctttgatgat agttttgaag aaccacaaaa aacggtttat
    11461 tttacaaaac gtgtgacaaa ggcaataggt ccagaagatg agatagatgg tcattttgag
    11521 ataaataatc aactagacta tgaagttgaa cttggagtag taataggtaa aacaggtgtt
    11581 aatattagaa aagaagaggt agaagattat atctttggtt acactgttat aaatgatatt
    11641 tctgctagaa agttgcaaaa agaacatgtt caatggttta gaggaaaagg attagataca
    11701 tttacctctt taggtccatg tattttacat aaaagtgcag ttccttttcc cataaaactt
    11761 aaagtatgta gtagggtaaa tggtgaagaa agacaaagtt ctaatacagg gttattcata
    11821 tcagacgttt cagacatagt ttcagaactt tcaaaaggta tgactttaga ggctggagac
    11881 attatagcta ctggaacacc ttctggagta gggatgggat tttcacctcc tcgatatatg
    11941 agtagtggag atgtagttga atgtgagatt gagagtattg gaattttaaa aaattacatt
    12001 aagtaactgt tttaatataa ttaatattaa agctaaatct gatgtattaa tgaacatatt
    12061 aaaataaatt ttaggagttt aaattatgaa agcagaattt ttgacaccag ttgttacaat
    12121 atttgatgaa gaaggaaatt tagataaaga aggtaataaa aaagtgtatg accatcttat
    12181 agatggaggt gtagatggac tagtcataat gggaagtact ggagagttct ttaatatgtc
    12241 tatggatatg caaaaagaac ttatagattt agtaactagt catgtaaata agagagtaaa
    12301 agtgtttata ggtacaagtc gtatgtgtat aaatgaatct atagaacttt caaactatgc
    12361 acataatagt ggagcagatg gtgttatgat aataagccct tattatttct ctcttgatga
    12421 tgatagtatt gagttatttt atagtgaaat agctaagaat acagaggctg aaatatattt
    12481 gtacaattat ccagatagaa ctggttatga tttaagtcca gaacttacat taagacttgc
    12541 aagaaaacat aaaaacatag ttggatataa ggatacagta actttgatgg gacataccag
    12601 agatttaata aatactatga aaatagagtt cccagaattt aaggtttata gtggttatga
    12661 tgaaaacttt atacataatc taatgtctgg gggagcaggt tgtatagggg ggttatcaaa
    12721 tttaattcca gaagtttgct ccaaatgggc tgaagcttac aataatagag atatggagaa
    12781 ggttatagaa attcaaaaat atattaataa agcaatggat ttttacttaa taaccaatcc
    12841 atttatacca gcaatgaaaa aagctatgaa tatcagaggg ttacaaataa gtgattatat
    12901 gactgctcca tttgtaaaac caaatgaaaa acaagtatgt gaaattaaaa aattgatgaa
    12961 tgaattaaat atatgttaaa tcaaccttgc aaagaggtca acaataaatt acaattatct
    13021 taataaaaat taaattaaaa taatactagg gtactatatt agtttaatag ggaaagtgat
    13081 gaaaattcac tacagccccc gctactgtat acggatacga aatcaaaatc cactgaaatt
    13141 tataaaaatt ttgggaaggg tgatggagga aaagccgtga gtcaggagac ctgcccagta
    13201 ttatataagt tcaatttagg gtggagattg taatttataa taaaatgaag tatcaattcg
    13261 tttttgtaaa gttgatatat gaacttctcc ttattaaatt aggagaagtt ttttatttta
    13321 ttaaaaggtt atacttatta aagatgactt taggataact ttaataagta tactttaaaa
    13381 agcatatatt attaaagttt tttagccact cataaaaaat aagatattgg agtgaaagag
    13441 atgaaattta aatcaaatgt tttaaagcag ttttttttaa ttgctatggt aatttttatg
    13501 gtaggaggtc ttatagggtg tagtaaaagt gacaaagaga ataaacaagc tagtactggt
    13561 aagaaattag aattaaaata tgctagtgga tttagtgttg aagatttaga agatggaatt
    13621 aaaaaagtaa ctgatggaga taatcgagaa ctgattttaa tcccaaagaa gattaaaatt
    13681 cctgaaaaat ataaaaatgc taatatagtt agaacgcctg tggacaatgt attaatagca
    13741 tctacaacac aggcttgttc tctaagaaca atagatgaac tagatagtat aaaagccgta
    13801 acaacacaag agcagtactg gactattaaa gaaattaaag atttaatgaa agatggaaaa
    13861 atacattacg ttggttcaaa tttagcacca gattatgaaa aaatagtaga cttaaatcct
    13921 gacttaacta tagtgagtag tggtttatca gaggctgata caaaatttat tgacaaatta
    13981 aaagaattag ggcttaatta tgttgtagac aatgagtata aagagcaaaa tccatttgga
    14041 agaatggagt ggacaagttt aatagctgca ttttatgata aagaagatat ggctactagt
    14101 gaactaaata aaactgtaca aaaggtagaa gaagtaagta aaaaaataaa aaacaaagct
    14161 aaacctaaag ttgtatgggc atctgtatat gaaggaagtg cttatatagc tcctaaagat
    14221 tcatatgtgg ataatatgat aaaaatggct ggtggaataa atgcatgtgc ttcaatagat
    14281 tcaaatgaag ggcgagtaag tattgaacaa ttgcatgaag ttgcaaaaga tgcagatata
    14341 tttgtatact catcaagttc agattatgca ccaaatttag attctataaa atctagtgca
    14401 cctgtattgg ctgatttgga tgttgtaaaa aacgggaacc tatgggtgtt tgctccagat
    14461 tattatcaat cagtagataa gacagacgaa ttaatagtag atttagttga gatgttccac
    14521 ccaggtacta caggtgaaaa aatcaaacat tatataaatt atgataaata gtacagagag
    14581 gacacaaaaa gataggtttt atagatttta gttaatatag ctttgctaaa aggagtatac
    14641 aaatggattt tatcaataaa atgagaataa ggaatacgac aatatttata gtattaatta
    14701 taagtatgat acttactttt ttagtgtgtg tagcatttgg ctctgtaaaa attgaattta
    14761 aagaagtaat agatgttgta ttaagaggta tggataatac agaacattat aaaataatat
    14821 acaatataag aatccctaga gtacttgcaa ccatggtagg tggagcatgc ctagcagtat
    14881 caggtatttt attacagata ttttttaaaa acccaatagt tgaaccgtat atattaggag
    14941 tatcttcagg ttctacttta tcagtagctc tcatgatttt gggaggatac acattgggat
    15001 ttgagagtgt aaattcaatg agtatatttg tagcagcttt tataggagct ttaattgtaa
    15061 tgtctatagt cattttattt gcaaataagg taaagaatat aacaactctt ttaataatag
    15121 gaattatgac cggatatgta tgtagtggta taacaaatgt gttacaatca tttgcaaata
    15181 gtgataaatt aaaagaattt actatgtggt ctatgggaag tttttctgga tttacatggg
    15241 aaaaggtaga aatacttata atagttggta tattaggtct tttgatgact gtattcataa
    15301 taaaaccttt aaatgctttt ttactaggag aagagtatgc aaaaagtatt ggcataaata
    15361 taagattttt tagagttttg atagtattta tatctagtat attagcagca atagtatcag
    15421 catttgcagg acctataggg tttattggac ttgcagtgcc acaaatttca aaaatgatat
    15481 ttaaaacatc agataataga gttttgatac cagtagtaat atttttaggc agtatagtta
    15541 cagctctttg tgatttaata tcaagagtaa ttttatcacc agtagaatta ccaataagta
    15601 caataacatc tttattagga gctccaattg tagtaatgtt attggtgaaa aggaaggata
    15661 gggcttagac atgttgaaaa ctaataattt aagtgtagga tacaataaca aagttgttat
    15721 aagtaatata aatgtagaag taaaaaatgg agaaatacta tgtcttttgg gaagtaatgg
    15781 agctggaaaa actaccctat taaggtcctt atctaaactt atatcaccaa taaaaggtga
    15841 aatatatttg aatggtgtaa acataaattg tatatcaaga aaagcattat caaaaaaaat
    15901 ggctttagta cttacaaata gattattagg tgatttaatg actgtacaag atatagtcaa
    15961 tataggaagg tatccatata caggattttt tgggagtcta tctaagaaag atttaataat
    16021 ggtagatgaa gcattggagt ctgtagatgc cttacattta aagaaaagat attttgatga
    16081 actaagtgat ggagaaaaac aaaaggtgtt ggttgcaagg gctcttgttc aagaaccaga
    16141 aataattata ttagatgagc ctacaactca cttggatata aaacatagat tggaactcat
    16201 aaacatactg aaaaaattaa gtaaagaaaa gtcaatatca gtaatacttt cattgcatga
    16261 aatagacata gcattaaaaa gttgtgataa agttgctctt ataaaaaata ataaagtcat
    16321 agcttatggc cagcctgaag atgtagtaga tgaggatatt ataaattcgc tttatgaatt
    16381 ggatgataaa aactttaata gtcttttagg ttcagtagaa attagtaata aatctaaaaa
    16441 tgaagttttt ataattggtg gtgggggtaa agcaacacca atttatagag cttttactaa
    16501 aaaaggtata ggtctgtatt ctggaataat acatgaaaat gatattgatt atgaaattgg
    16561 tagaactatg gggataaaga tgtttacaga aaatccattt gagcctataa gtgatgagag
    16621 tttcgatttg gctattagaa atttaaatga ttccaagatt ataatagata caggatttag
    16681 tgtaggagag acaaataaga ggaatataga tattataaaa gaagctttaa aattggataa
    16741 aaaagtttat tcttttagaa atagagatga gagtaagaag tattatgaca gtttggatag
    16801 taagatagaa catatagata aagtttctca aataatcaat agtgcagata taaataattt
    16861 attataattt atgaaataaa atgtacttat gtgttataga aaaaagagta tagattataa
    16921 tttttttgaa attacaatct atactctttt gttattttgt ttatgtattt atatatttat
    16981 aaaactttag cattttatag ataaccaata gcttcctctc tagttaaaaa aaagtgaatc
    17041 cctgtagtcg attctttcca tctattttca ttaaaatctt cagctacaat cgtttctcct
    17101 actctatata taaaattttc atcagcatat gcacgagcac ttttaaaaga ttctttacca
    17161 tctacactag taattgacag tacttttgcc ttatcacatc tacaagcatt tgatgtagca
    17221 gacactcttt ttgcgtcgct gggtattaat aattgtacca ttctaaaatt aaaacatttt
    17281 ttatagccta taaatgctcc tgtttcagga caatacaatt taaaaaaacg agtactttca
    17341 tctgatatta ctccatttaa agtagcatat tctaaattgg aattttctaa attagctcct
    17401 ctaatatctg agttagataa gtcacaaaat ctaaaacttg caccacttaa gtctgcattt
    17461 tttaatatag aattttttaa tgaacaatcc gaaagataag aattattaaa aatagagttt
    17521 tctaaatctg ctttttctaa atttaaattg ataaggtcag accacgaaaa gtcaatatta
    17581 cttaagcagt atcctcttaa gtccatattt tctaatctta attcttttaa atctgccttt
    17641 tctccagcat tattgttttt aagccataaa taatgtttct caagtgtttt ttctaattct
    17701 tgtttagaaa tagttttcat taaaatcacc ttcataaatt tatttttata tatgtagtag
    17761 caaaatagtc atatatttaa ttatataaca ataaataaaa aacttctaaa taatatataa
    17821 aatatcatct tgaaagtact tttttcaata tatcagaata tatttttaag ctatttagag
    17881 ataatttaaa tttttaaaca ggacataata agcttgtatt gttttatatt tgagttgata
    17941 aaaatttctt taaataaaaa taatgtcaat tataaagtaa taaaagttta gatataaaaa
    18001 aatttgtgta tatactatat gtagtatata atagataaag cactgactat atgtagtgct
    18061 tttgtttttg aaaaatactt attgcatttc tataattata gattaatttt gggatttcaa
    18121 ctgataagta ccaaaagaaa taataagcaa attaagtgaa attatggcac aagattatgt
    18181 taaaaggacg gtaaataatg aattggatag agcttaacaa tcaagtgata attaaaaatg
    18241 aacaaggcaa gtaccaattg gaaaaggata aggaagcctt aagtagttac atagaagaat
    18301 ttatacagcc aaagttgaga aaatttaata acctagaaga aagattgaag tatttaatag
    18361 atgaaggtta ttattcaaaa gaagtaataa atcagtatag catagatgtt attaaaaata
    18421 tatatgatat gattgataaa cataagtttg aatttcaatc atatatgagt gcaaataagt
    18481 tttatcaaaa ttatgcatta aagagcaatg atggaaagga aatattagaa acatataatg
    18541 ataaagtgtt aattgtagct ttgacactag gaaatggcga taaagattta gctttaaact
    18601 tggcagataa aatagtaaaa caagaatttc aaccagctac acctactttt ttaaatgctg
    18661 gaagaaaaag ggctggagaa atggtatcat gttttttact atctgtagaa gatagtacag
    18721 aaggtatatc gtatgctata tcttcatcaa atcacttatc aaagattggt ggaggagttg
    18781 cactaaattt atctaaactt agagcatctg gcgaatctat caaagatata gaaggtgcag
    18841 ctggaggtgt tgttggagta gctaaaatgt tagaacaatc atttagttac tttaatcaaa
    18901 tgggagctag acaaggttct ggtgcggtgt accttactgt attccatcct gattttgaat
    18961 tgttgatgga tactaaaaag ataaatgcag atgaaaaaat aaggcttgct acattatcat
    19021 taggagctat aataccagat aaatttatgg agttggcaga aaaaaatgaa gtagcatatg
    19081 cattttatcc tcatactgtg tataagaaat atggagtttc tcttgatgag atagaaatgg
    19141 ataagtggta tgataaactg gttaatgata gtgatataag aaaaaaagaa ataaatccta
    19201 ggcagatgtt gacaaaaata gcacagatgc aacaagaaag tggatatcca tatgtagtgt
    19261 acatagatac agctaataga gaacatacat taaaagatgt tggaattatt aaaatgtcta
    19321 acttatgttg tgagatattt caatatcaaa caccttcaga aatagagggg tatggaggaa
    19381 aaaatgaatg gggacaagat ataagttgta acctaggttc tttaaacata gccaatgtaa
    19441 tggataataa aactatagaa agtacagttg aaacagcaat aagagcactg tcatttgtag
    19501 cagatagcac agatataaaa ccagtgccaa cggtaagcaa tagtaacagc aagtctcatt
    19561 ctataggatt aggagctatg aatttacatg gatacttagt aagagaaaat attttatata
    19621 cttcagatga tgctatagat ttttctaatg tattttttgc aatggttaga tactattcaa
    19681 ttaaagcttc tatgaaaata gctatagaaa gaaatcaaac ttttgaagga tttgataaat
    19741 cagaatatgt taagggaaga aatagcaagg ttttatcaaa atactatgaa caatcttatc
    19801 tacctaagtc agaaaaggta agagcattat ttgaagggat ttatatacca acaaaagaag
    19861 actggacaaa attattggat gaggtaaaag aaaaagggat atataatgca tatttaatgg
    19921 cagtagcacc aactcaaagc attagttatg ttcaaaatgc tacatcaagt ataatgccaa
    19981 ttactgagcc agtagaagta agaacatatg gagactctac tacaatatat ccaatgccat
    20041 ttttaacaaa tgataatatg ttgtattatc aatcagcata caggatggat atgagaaagg
    20101 tcatagattt agtggcaaca gttcaaaacc atgttgacca aggtatatca acaacactgt
    20161 ttgttacaga tgaaaagaca actagagata tagctagaca ttacatatat gcctataaaa
    20221 aaggtttgaa aagtttatat tacacaagaa caaagatgac aagagatact catgagtgtc
    20281 tagtgtgttc agtataggga gagaaatata tgacatttaa tttacataaa atacataatg
    20341 cagttaactg gaatagagaa gaagatggat ttacacaagc tttttgggaa caaaatgtaa
    20401 aacaattttg gcttcctgaa gaaatatctg tttctaaaga cataaaggta tggaatgaac
    20461 taagtaataa agaaaaggaa ttatataaaa aagtattggg tggtttaaca ttactggata
    20521 caaaacaggg gaataatgga ataccatcta tgatgagttt aacagaaaac ctacaaagaa
    20581 aagctgtctt gtcttttatg gggactatgg aagaaataca tgcaaaaagt tattcttcta
    20641 tatttatgac tctactttct aatttagaaa tagatgaatt atttgaatgg atagaaacag
    20701 aaccaacatt acaaagaaag gctgatttag ttttagctca atacgaaaat actacaaatc
    20761 aagagggatt atacctatca atggttacaa gtgtattttt agagagtttc ttgttttatt
    20821 caggattctt ttatccattg tatctatctg gtcaaggaaa gatggtagct agtggagaaa
    20881 taatatcgct tattcttaga gatgagtctt tacatggtaa gtatatagga ttgctagcac
    20941 aagaaatata cgattcattt gataaaatgg ataaggagat gttagaagag aagatgtatt
    21001 ctatacttta tagccttatg gagaatgaga tagagtatac aaatgttata tacagagaaa
    21061 gtgggcttga aaaagaggtt gtgaactttt taaaatacaa tgcgaataga gctttagaaa
    21121 atctagggtt tgagaagcta tatactgtag atgctataaa tccaattgta ttaaatggtc
    21181 ttagcactga aaccaaaaca catgattttt tttcaactaa gggtaatgga taccaaaaag
    21241 gagtgtatga ggaattagag gatgaagatt ttataatata atttataatt atgacacact
    21301 gttgtcactt ttagcgtgat aatatcaatg tataaaatat atatggaggt atttttatga
    21361 aatatgaatg gagaaaaaga gataaagagt tatatttacc taaaaagaat cctgtaatag
    21421 ttgaaatacc agaacagaaa tttatcatgt taaaagggag tggagaccca aattctaaag
    21481 aatttactga agcaatagga gtactttatt ctttggcata tgcaataaaa accatgccta
    21541 aaaaaggaat tgtaccagaa gggtattttg actatacggt ttttccttta gaaggtatat
    21601 gggataaggg agaaaaaagt aaaggcttag atgtacttat taaagaagat ttaatttaca
    21661 ctataatgat acgtcagcca gattttgtta cacaagacct gttggataaa gctactgata
    21721 tagtaaagaa gaaaaaacct cataatcttc ttgaaaaagt tagatttggg tctattaatg
    21781 aagggctatg tgttcaaatg cttcatatag gttcatatga taatgaacca gaaagctttg
    21841 acattatgaa cgaattttgc aaaaaaaata aacttataag aaaatcttac acacatcgtg
    21901 aaatttatat aagtgatgct agaaaagtat cgcctgaatc attgaagacg gttttacgtt
    21961 ttgaagtaga aagggaagta taaatatttt gaataattta taagagttat aaaaagtgaa
    22021 gtctgttgag acttcatttt ttgatgcaat tttaaaatta aatttgatgt ttcataatat
    22081 tttaaaaact ttctactttt attagtgtga ataactgttg ctattaaata ataata
//
LOCUS       DAMJVK010000066        21506 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.067, whole genome shotgun sequence.
ACCESSION   DAMJVK010000066 DAMJVK010000000
VERSION     DAMJVK010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21506
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.067"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(79..957)
                     /locus_tag="PC414_002520"
     CDS             complement(79..957)
                     /locus_tag="PC414_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDF2464908.1"
                     /translation="MRYDIAIIGSGPAGLSAAINAKIRNKTIIMFGNDNLSNKLVKAP
                     SIDNYLGFYDISGDELKDKFKSHIDSMDISIENKRINNIYAMGEYFTIMSGNDMYEAT
                     TVILATGVEYTRPIKGEEEFLGRGVGYCATCDAPLYRNKKVAVIGYNEESKEEANFLS
                     ELTSKTYFIPMYKKDNLMRSSDNLDDSIEVIHDRPVQIDGDKLVNKVSFKENHIEVDG
                     VFVIKDSTAPSALVPGIEIDGIHIKVDNNMKTSIDGCFAAGDCVGKPYSYIKAAGQGQ
                     IAALNAVYYLDKLKRA"
     gene            complement(974..1291)
                     /gene="trxA"
                     /locus_tag="PC414_002521"
     CDS             complement(974..1291)
                     /gene="trxA"
                     /locus_tag="PC414_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDF2464909.1"
                     /translation="MAKVINTSEFRSSVEGSKGVVVVDFFATWCGPCNMLGPVFAELG
                     EEMKDKARFVKVDIDESLEIAQQFNVSTVPTMIIFKDGKPVETLIGFMPKNKIEMQVK
                     SYL"
     gene            complement(1441..2673)
                     /locus_tag="PC414_002522"
     CDS             complement(1441..2673)
                     /locus_tag="PC414_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2464910.1"
                     /translation="MINKNVFTSTKNHLIKMYIIVVGSFLIIFSIFIYSYFRGLTYSG
                     IDSEINDELEYIVSQFKRTSFLNPIRLKDPKDMVYVYEDGRISYYTQNEYFDELLPDR
                     RLDKKNSFFKYTENGYTFRELNVDVGRYQIQIIRNIDSEMNSLRQLTSVLIIGILISV
                     IITYFVAVYLTRKALIPIETAWKNQAKFIQDASHELRTPITIVSSKLESMLKSPESTV
                     NDEVETIATAMKETRRLKKMITDLLSLTKEDSIVKVNLEEIDLEKLLEEISEDYIDIA
                     EFQEKRFVFNSKLKNKVIITDKNKLRQLILIFIDNAFKYTKLGDEIALELKEDIEDEV
                     TLLISDTGIGIKKEEIPLIFDRFFRSENVRNKDLEGSGIGLSIARMISLNLSIDINVT
                     SDVDIGTTFELSIPKKLK"
     gene            complement(2663..3334)
                     /locus_tag="PC414_002523"
     CDS             complement(2663..3334)
                     /locus_tag="PC414_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2464911.1"
                     /translation="MKVLIVEDNKILLESVVEELSKHFETEKCEDGEEALYLINQNIY
                     DLVILDLMLPNINGFDILKKMRINNIDTPVLILTAKETLDDKVEAFTIGANDYLTKPF
                     YMEELVARVYAILRTNGKIKERNGLEFKSLYLDTLEKRVYIEKEEIKLQNKQFNLLEY
                     FVLNKGSILLKEQIYDRIWGIDSDATIEIVEVYVSNLRKKLSKYGYDKYIKTKRKVGY
                     IFDDK"
     gene            complement(3350..4186)
                     /locus_tag="PC414_002524"
     CDS             complement(3350..4186)
                     /locus_tag="PC414_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464912.1"
                     /translation="MNKIVKKSISLMIILTIFIFMLTACEKDEQPDSVDIQTEDKNEI
                     KIDEGNAKVLNIGKSEIINIDEGDKVDTSTKVENNSTFNISNVELIYNEYDENKKIIS
                     SDSKALLDMTLMPGKVAYIECGHKTFVKGVEVYAYEYEAEGKIVYVNLKENTINIRNN
                     NIKLENSSQYEVLSTSELKKVNESNEGITYQVKVKNSSSKDLGNIILKTAEVNENGEY
                     LTVNRVPSYKILKPSEETDIDILCSTKAKSVEIVGYTYDDIKEKANVDIDLKSHKVKI
                     DK"
     gene            complement(4212..4886)
                     /locus_tag="PC414_002525"
     CDS             complement(4212..4886)
                     /locus_tag="PC414_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDF2464913.1"
                     /translation="MNNNEDRIDKFLELQSKGFKFAEIAQNLKISQSTLRTFLNKKGY
                     KSEKGKYILKDELNYNKSLDNRNLEEEKAKEYEKSKKNTISNRKKVSTKKKISNEKAL
                     SKSVEQIEFNDTGLKNAKPKQNIKPKKDRKINITQEDLDKLCEVYDWYLEVKDYNIKP
                     KKVSNKKDVHIDDEHIDELKTTRIRIDKEIWNDFERLCSNSNFEKHQILTQALKEFMK
                     KYKNLL"
     gene            complement(4900..5628)
                     /locus_tag="PC414_002526"
     CDS             complement(4900..5628)
                     /locus_tag="PC414_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HDF2464914.1"
                     /translation="MMFDRGKWNKLDYNELLEYMMSIQDIKYRDFNKKLIPGTENIIG
                     IRVPNLRKLSKEISQGNWKEFLEVAQDTYYEEVRLQGMVIGNINSNFEETLYYVKRFI
                     PKIDNWSVCDGFCSDLKSMKKYKENMYDILKKYVYSKNPWEIRFALVMFLIYYVDDKH
                     IKEIFEYCNNIQSEEYYVKMGMAWLLSICFIKCEEETFLYIKNNNLDDFTYNKMLQKI
                     IESNRVDLEKKNIIRSMKRKTRRC"
     gene            complement(5755..6444)
                     /locus_tag="PC414_002527"
     CDS             complement(5755..6444)
                     /locus_tag="PC414_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2464915.1"
                     /translation="MDRYSEITNKNQREIVLLKGFPCIWGKCSFCDYIDDNSNLEEEM
                     NKLNLKVLKNVTGKYGVLEVINSGSCFELPKDTLEKIKCIIKEKNIKKLFLESHWSYK
                     NRLKEMREYFEIPVVFKIGVETFDNDFRNNILNKNANFKTPQDVKEYFDSPCIMVGIK
                     GQTKEMIDKDIEIILNTFEKATVNVFINNSSSIKRDEELVKWFVSKYKFLDENPNIEV
                     LYNNTDFGVGD"
     gene            complement(6452..7015)
                     /locus_tag="PC414_002528"
     CDS             complement(6452..7015)
                     /locus_tag="PC414_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDF2464916.1"
                     /translation="MKRKICVKTITLIAFGISINIIGAFIAMGLRLPVYLDSIGTIMI
                     ASLLGPKYAVVTGVFGSLISGITFDVYSLYFAPVQISTGLLAGIAFKKNFLRGLKTPL
                     GVLLFAIPTSIISSIISAFLFGGMTSSGSSYIVQILKVLGLGDVFSVFVTQVFTDYGD
                     KLLAVVLVNLGLNAVPKTLKVSLTGGK"
     gene            complement(7017..7985)
                     /locus_tag="PC414_002529"
     CDS             complement(7017..7985)
                     /locus_tag="PC414_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HDF2464917.1"
                     /translation="MEKRKVIIDCDPGIDDSLAILLALNSPELEVIGITTCCGNVPAN
                     IGAENALKTLQMCSSLNIPVYIGEEAPLKRKLVTAQDTHGEDGIGENFYQKVVGAKAK
                     NGAVDFIINTLYNHEKVSIIALAPLTNIAKALIKDKKAFENLDEFVSMGGAFRIHGNC
                     SPVAEFNYWVDPHGADYVYKNLSKKIHMVGLDVTRKIVLTPNIIEFINRLDKKMAKYI
                     TEITRFYIDFHWEQEGIIGCVINDPLAVAYFIDRSICKGFESYVEVVEDGIAMGQSIV
                     DSFNFYKKNPNAIVLNEVDEKKFMYMFLKRLFKGYEDIIDSVEGVI"
     regulatory      complement(8086..8129)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            8495..9124
                     /locus_tag="PC414_002530"
     CDS             8495..9124
                     /locus_tag="PC414_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2464918.1"
                     /translation="MSIKERRKNEKEEMKKKIMDASIEIINQHGYENLSIRKIATKIE
                     YSPTTIYLYYKDKAEIISDMTNKLYNTVESNAIDIMNNCSSLPIDKQIIEIMTSFIKT
                     LSSEPEMAKSIMHSRMNIIFASENTNNTPSNNGIKMLDKFLSIGIEQGIFKKNIDKSS
                     WMLISALLGFVLCVVENKLYSLHNFSQLIENFLDILVGGLYNENSQQGY"
     gene            9099..9503
                     /locus_tag="PC414_002531"
     CDS             9099..9503
                     /locus_tag="PC414_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HDF2464919.1"
                     /translation="MKIRNKATNKKKKYVKISIALFFVFICIIIVIVSNLKPENLVVK
                     DIDINNVKNGIYTGSADNNLVKATVSVEVNNGKIQNINILKHDHLLGKPAEKITTSII
                     KQQSLDVDAITSATYSSNTIRKAVENALRKGE"
     gene            9506..9982
                     /locus_tag="PC414_002532"
     CDS             9506..9982
                     /locus_tag="PC414_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HDF2464920.1"
                     /translation="MNTIIIYSSKYGCTKDCANILKNKLSDNVTIVDINNNNNKIELS
                     KFDKIIIGSSIYVGSVSKKIQVLCNDNVELLNKKQVGIFLCCGFSEQADKYLKSNFPS
                     SLLESANAIGIFGSEARLEKMKFLDKLIMKAVSKGNYDSFRISQDNIDNFLINLNS"
     gene            10554..11219
                     /locus_tag="PC414_002533"
     CDS             10554..11219
                     /locus_tag="PC414_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HDF2464921.1"
                     /translation="MIKLIGILIVVIGFILKIDTLFTVLLAGVATGIVAGLDFNQIFT
                     ILGDSFVSNRGVSLFILTLPVIGVLERYGLRQRAVSLIEKLKRLTTGKVLTIYMIARQ
                     IAGALSIRMSGHPQFVRPLVNPMAQAAGLSNSDELKESDEEAIKALSAASENYGNFYG
                     QNLFAGSSGVLLIASTLTQFGYNVTGLNIVKASTIMAVIALVVATIQFTLYDKKLNKS
                     HKK"
     gene            11241..12173
                     /locus_tag="PC414_002534"
     CDS             11241..12173
                     /locus_tag="PC414_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HDF2464922.1"
                     /translation="MQNFINISLEIFYALMGFLMIVIAYKSFTTINNNKKYGTSLFWI
                     LISLPFIFGRLIPANIIGIILILSSLLTLSKQVVFAKYEEPDENFGKEQADKLKNKIF
                     IPSLILAFAAVVVAMSLSNFDNSSQFAIGVGSIIALISALIITKAKPATSVQDGSRLL
                     QQMGPASMLPQLLVALGALFTQAGVGEVISTMISGVVPADSRLFGVIAYVLGMVIFTM
                     IMGNAFAAFSVITAGIGIPFVLSQGGNPAIIGALALTAGYCGTLLTPMAANFNIVPAA
                     LLECKNDYIVIKYQAPVALVLIIAHILVMYFLGF"
     gene            12194..12835
                     /gene="pcp"
                     /locus_tag="PC414_002535"
     CDS             12194..12835
                     /gene="pcp"
                     /locus_tag="PC414_002535"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HDF2464923.1"
                     /translation="MKILLTGFDPFGGEPINPAQEAVERVNNNINGAEIIKITIPTVM
                     TKSVEAIDKAIQEHNPDIVISVGQAGGRFDITPERVAINIDDFRIKDNEGNQVIDTII
                     KEDGEPAYFSKLPVKAMVKHMNENKIPASVSNTAGTFVCNHVMYGILYMIDKKYPNIR
                     GGFIHIPYTTSQVIDKKNTPFMSLEEIVKGLELAIEACIIYKEDVKEIGGEIS"
     gene            complement(12955..13287)
                     /locus_tag="PC414_002536"
     CDS             complement(12955..13287)
                     /locus_tag="PC414_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2464924.1"
                     /translation="MSIDCISNTDLSETGFSYTLSLISGKYKMIILYCLVEFEVVRYN
                     ALKRYINTISYKTLSLSLKELEADNLIIRTEYPQIPPKVEYSLSERGKSLIPILDAMC
                     EWGERNRP"
     gene            13553..14173
                     /locus_tag="PC414_002537"
     CDS             13553..14173
                     /locus_tag="PC414_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2464925.1"
                     /translation="MKVLLINGSPNQYGCTYTALNEITKVLSKHDIKTEILYLGKETI
                     PGCISCASCFETGKCIRNDKVNELIEDLDNIDGIIIGSPVYFSSATGQLTSFLDRLFF
                     IAGSRMATKLGASVVSCRRGGASATFDQLNKYFSISNMPIVSSQYWNQVHGFTPEDVM
                     KDEEGLQTMRTLGENMAWLLKCISAGKKAGIKEPQYEETIMTNFIK"
     gene            complement(14498..15322)
                     /locus_tag="PC414_002538"
     CDS             complement(14498..15322)
                     /locus_tag="PC414_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HDF2464926.1"
                     /translation="MDFFKKGISGFTIKILALIFMTFDHIAAFMPQTMQIPIWFHWVG
                     RISAPLFIFMAVEGFYHTSNRKKYISRLYIWSVIMAIGNQIINNVFSHPEGAIIINNT
                     FSTLFLIAIYLQAIEFIKKFRKEKEIKYFIIGLLMIIIPIILGIFTVALLFKVTNRVI
                     ALFMILVPVPFLVEGGPIWIILGIIFYLCRGKKFSLSICYVLMCIFIFTTMSNGDYSL
                     KNSILQNYQWMMIASLPLMLLYNGEKGKSMKYLFYLYYPIHVYILYILGIYLINGF"
     gene            complement(15333..16736)
                     /locus_tag="PC414_002539"
     CDS             complement(15333..16736)
                     /locus_tag="PC414_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2464927.1"
                     /translation="MKWKITRNFIFTIVFVAISVVIINIISILYVISTNSFFKVVDSG
                     NNPEEFARSFEKDLYEKDGEFKLSKIGAEKLEKSNSWIQVLNDLGEEVYGVNVPKDTP
                     KKYTPFQMVNNYKYIETKYVNFVLEKYLNKKHLNIIVGIPSRDISRIILTYSQNNIKK
                     TLNKVIIITLVIDSVVALGVGYLFSRKLTKPISSVLWSIETMANGNYSLYLKDRGIYE
                     EVFKNINMLADTLRVNEVERKENEELREEWLANITHDIKTPLASIQGYAEIINDKDYE
                     FEEDEIQEYTEIIYNKSKYIKDLVDDLNLSTRLKNNTIVLDKKKINLVSLVRDIIIDI
                     LNDNRYKNRNIEFESNEDLIEVYVDSILFRRAITNLIFNSIVHNSEGTLISVEIVKKD
                     NIEIIIKDDGIGISKSDLKHIFKKYYRGTNTGEMHKGSGLGMAISKEIIEIHKGKIYV
                     SSEIGIGTKIIIEIKQN"
     gene            complement(16740..17465)
                     /locus_tag="PC414_002540"
     CDS             complement(16740..17465)
                     /locus_tag="PC414_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012450879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2464928.1"
                     /translation="MENRVLIIDDEVEILKLLETVLKKEGLNNIYTAKTKKEGLELFK
                     SINPDLIVLDIMLPDGEGYDICKEIRKTSNSPIIFLSAKTEELDKLLGLAIGGDDYVT
                     KPFSPKEVAFRVKAHLRRLSYFSDAQNESKNLNNNEEKIISFGPYILNESRAELIKDG
                     KSIGLSAKELKILSLFAHNQNQIISKEKLWDKVWGEDYVGFDNTIMVHIRKIREKLED
                     NPSKPEYILTIKGLGYKLAVKED"
     gene            complement(17577..18350)
                     /locus_tag="PC414_002541"
     CDS             complement(17577..18350)
                     /locus_tag="PC414_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2464929.1"
                     /translation="MMYLVKAEMIKILKSKSVWIIWLFLIFFGFFLIRKFDVLDTYAD
                     IFYKIEGSIPLIGLIMFIITSGNYIKEYDSNMTGLINTTKKGKKSVVLSKLVANGLIL
                     SIINISFILLVGIRGFSFFDFKNLNESIHNLWYFGNSNLNLTVIQMYIIVILTTIFAS
                     FIFAQIGLTLSSIFKSAVIPFILGGLIMAIPYFSVGFIPDKAIKFMSVTPNWIMMSQQ
                     MVKYNVPSILIVFSIVISIILMIVLTKITYENFTSSKRF"
     gene            complement(18356..19243)
                     /locus_tag="PC414_002542"
     CDS             complement(18356..19243)
                     /locus_tag="PC414_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2464930.1"
                     /translation="MNKLEIENLSKIYGKKVANDKITVTLENGVYGLLGPNGAGKSTL
                     MKQITTLIKPDEGQILYNGEDIFKMDADYRNILGYLPQEFGVYKNFTAKEFLQYIGAL
                     KGLRGKYLNSKIGELLDLVGLYDVRNKSIGKFSGGMKRRVGIAQVLLNDPKIIVLDEP
                     TAGLDPQERARFRNLIAKMSRDKIIILSTHIISDIESVAKETIMIKDGKLLMKGTHKD
                     ILSHMENKVYNVQVKNECEVDDIQSKYKVVSIQSDIDSVVLRIVSEDIPSEDNIQPVQ
                     AKFEDVYMFYFDLEDAREV"
     gene            complement(19257..20279)
                     /locus_tag="PC414_002543"
     CDS             complement(19257..20279)
                     /locus_tag="PC414_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464931.1"
                     /translation="MVNWEIKKLAKSKSMFISLGILVLILMISIFITPVLETESYYID
                     KEKGRIEDTRSGIDVGNEKFQNKINVLKQFSNQTDTGEFSKKIELMSKKKLDNLSVNE
                     YKDISFWKVFNYRVTNSLINVGMLIIISIIISNIYVDEVVTSVKDIILSSKEKKKALK
                     SKIFVALLVPLVIYSVYLMGIFIITCMQQGVPVNGDLESFRIVDNVVALKTNLSITKY
                     VILNIGIELLMFEGWAMIAIFGSFISKSSISSISFFIFIISITKIMSVIHILPKKLLS
                     VVSNVNYYDLIFGFNKIIGNYLGDIMIFKVNIDIVNIAIGLLMAILIGAMSLCFLVIK
                     SDYISR"
     gene            20854..21030
                     /locus_tag="PC414_002544"
     CDS             20854..21030
                     /locus_tag="PC414_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464932.1"
                     /translation="MFIGEIDKCTHILTAYISSSYDYCNFLDTQLDDFISEYGETVVE
                     TCLHQVLLLVSRYN"
BASE COUNT         7474 a         3380 c         2312 g         8340 t
ORIGIN      
        1 aaaaactgtc ttgaaataat aaaattattt caagacagtt ttttataaat atctttaaaa
       61 agttaatcaa tataaatatt aagccctctt taatttatct aaatagtaaa cagcatttaa
      121 agctgctatt tgcccttgac cagctgcttt tatataagaa tagggttttc caacacaatc
      181 tccagcagca aagcatccat ctatactagt cttcatatta ttgtcaactt taatatgaat
      241 gccatcaatc tctattccag gaaccaatgc acttggagcc gtactatctt ttataacgaa
      301 tactccatca acttcaatgt ggttttcttt aaaagatact ttgtttacaa gtttatctcc
      361 atctatctgt actggtctgt catgtataac ttcaatagaa tcatctaggt tatctgaact
      421 tctcataaga ttatcttttt tgtacatagg tataaaataa gtctttgaag tgagttcact
      481 taaaaaattt gcttcttctt tagattcttc attatatcct ataactgcta cctttttatt
      541 tctataaagt ggtgcatcac atgtagcaca gtagcccaca cctcttccta gaaattcttc
      601 ttcacccttg attggtctag tatactctac cccagttgca aggattacag tagtagcttc
      661 atacatatca tttccagaca ttattgtaaa gtattctccc atagcataaa tattatttat
      721 tcttttattt tcaattgaaa tatccatact atctatatgt gatttaaact tgtcttttaa
      781 ttcatcacca cttatatcat aaaaacctaa ataattatct attgaaggag ctttaacaag
      841 cttattactt aaattatcat taccaaacat gataattgtt ttatttctta tttttgcatt
      901 tatagcagca gacaatccag ctggaccact gccgattata gctatatcat atctcataat
      961 actcacccat tttttataaa tatgatttta cttgcatttc aattttgttc tttggcatga
     1021 atccaattaa agtttctact ggttttccat ctttaaatat aatcatagtt ggtactgtag
     1081 atacattgaa ttgttgagct atttctaaac tctcatctat atcaactttt acaaatctag
     1141 ctttatcctt catctcttca cctaattctg caaatacagg tcccaacatg ttacaaggtc
     1201 cacaccaagt tgcaaagaaa tcaactacaa ctactccctt actaccttca acactacttc
     1261 taaattcaga tgtatttata acttttgcca tttttaaatc ctcctattaa actttattat
     1321 ccctatataa ttatactatg aaatttataa atataaattt acttttataa ggtcttttta
     1381 tattttatac taattagtat acccattctt catttctaaa aacaattttt tatattttta
     1441 ttattttaac ttttttggta ttgacaattc aaatgtagtt ccaatatcta catcagaagt
     1501 tacatttata tcaatagata agtttaaact aatcatgcga gctattgaaa gtcctattcc
     1561 agaaccttct aaatctttat ttcttacatt ttcacttcta aaaaatctat caaatataag
     1621 aggtatctct tcttttttta tgcctatacc agtatcactt ataagtaaag ttacctcatc
     1681 ctctatatct tctttcaatt ctaatgctat ctcatcaccc aattttgtat acttaaaggc
     1741 attatcaatg aaaattaata taagttgtct taatttattt ttatctgtta ttataacttt
     1801 attcttcagt ttagaattaa acacgaatct tttttcctga aactcagcaa tgtcaatata
     1861 gtcttctgat atttcttcca ataacttttc taaatctatt tcttctaaat taactttaac
     1921 aatagagtcc tctttagtaa gagacaataa atcagttatc atttttttaa gtcttctagt
     1981 ttccttcatt gcagtagcta ttgtttcaac ttcatcattt actgtacttt ctggggattt
     2041 taacatactt tctaattttg atgaaactat tgttattggt gttcttaatt catgagaagc
     2101 atcttgtata aacttagctt gatttttcca tgctgtttct attggaatta aagcttttct
     2161 agtgagatat acagccacaa agtaagtaat tattacagaa attaatattc caattataag
     2221 aacagatgta agctgtctta aagaattcat ctcagaatct atatttctaa ttatctgtat
     2281 ttggtatcta cctacatcta catttaattc tctaaaagta tatccatttt ctgtgtattt
     2341 aaaaaacgaa ttttttttat ctagtcttct atctggaagt aactcatcaa aatattcatt
     2401 ttgagtatag taacttatcc ttccatcttc atatacatac accatatctt taggatcttt
     2461 gagtcttatt gggttcaaaa atgaagttct tttaaattga gagactatat attcaagttc
     2521 atcatttatc tcactatcaa ttccactata tgtaagtccc ctaaaatatg agtatataaa
     2581 tatagaaaag attattaaaa aactacctac tacaataata tacattttta ttaaatgatt
     2641 tttagtactg gtaaatacat tcttatttat catcgaatat atatcctacc ttacgtttag
     2701 ttttaatata tttatcatat ccatatttac ttagcttttt tcttagatta cttacatata
     2761 cttccactat ttcaatagtt gcatctgaat caattcccca tattctatca taaatctgct
     2821 cttttaataa aattgaacct ttatttaata caaaatactc aagcaagtta aactgtttgt
     2881 tttgaagttt tatttcttct ttttcaatat acactctttt ttctagtgta tctaaatata
     2941 aagatttaaa ttctaatccg tttctttctt ttatctttcc attagtcctt aatatagcgt
     3001 ataccctagc tactaactct tccatataaa aaggttttgt caaataatca ttagctccta
     3061 tagtaaatgc ttctactttg tcatctaatg tttcttttgc tgtcaatata agtacaggtg
     3121 tatctatatt attaattctc attttcttta ggatatcaaa tccatttata ttaggaagca
     3181 ttaagtctaa aatgactaaa tcatatatat tttgatttat taagtataaa gcttcttctc
     3241 catcttcaca cttttcagtt tcaaaatgct tgctaagttc ttctactaca ctttctaaaa
     3301 gtatcttatt atcttcaact attaaaacct tcataagtat cccctacctt tatttgtcta
     3361 tcttaacttt atgagatttt aagtcaatat ccacattggc tttttctttt atatcatcat
     3421 acgtatatcc cactatttca acactttttg cctttgttga acacaatata tctatatctg
     3481 tctcttctga aggctttaaa atcttatatg atggtacacg atttactgtt aaatattctc
     3541 cattttcatt aacttctgct gtcttaagaa taatatttcc taaatctttt gatgatgaat
     3601 tttttacttt tacttgatac gtaattccct catttgattc atttactttt ttaagttctg
     3661 atgtagatag cacctcatac tgactactat tttctagctt aatattatta tttcttatat
     3721 taatagtatt ttcttttaaa ttaacataca caatcttacc ctctgcctca tattcatatg
     3781 cataaacttc tacacccttt acaaaagttt tatgcccaca ttctatgtaa gcaactttcc
     3841 caggcattaa agtcatgtcc aacaaagcct tactatccga agatataatt tttttattct
     3901 catcatattc attatatatc aattcaacat tggatatatt aaatgttgaa ttattttcta
     3961 cctttgttga tgtatctact ttatcacctt catctatatt tataatttct gattttccta
     4021 tattcaatac ttttgcattt ccctcatcta tttttatctc atttttatct tctgtttgaa
     4081 tatcaactga atcaggctgt tcatcttttt cacatgctgt caacataaaa ataaatattg
     4141 ttaatataat cattagagaa attgattttt ttactatttt attcatatta agcacctcat
     4201 atagtagtat tttatagtag atttttatac tttttcataa attctttcaa tgcctgtgta
     4261 agtatttgat gtttttcaaa attcgaatta ctacataatc tttcaaaatc attccatatt
     4321 tctttatcta tacgtattct tgttgttttt aattcatcta tgtgttcatc atctatatgt
     4381 acatcttttt tattagatac ttttttaggc tttatattgt aatcttttac ttctaaatac
     4441 caatcatata cttcacaaag cttatctaaa tcttcttgag ttatatttat ttttctatct
     4501 ttcttaggct taatattttg ttttggcttt gcatttttta aacctgtatc attaaactca
     4561 atttgttcaa cacttttact taaagccttt tcattagaaa tctttttttt ggtgcttact
     4621 ttttttctat tacttatagt attcttttta cttttttcat actccttagc tttctcttct
     4681 tccaagtttc tattatccaa acttttatta taatttaatt cgtcttttaa tatgtattta
     4741 cctttttctg acttatatcc ttttttattt aaaaaagttc ttaaagtact ttgactaatc
     4801 tttagatttt gagctatctc tgcaaattta aatcctttgc tttgaagttc taaaaatttg
     4861 tcaattctgt cttcattgtt attcattaat attcctccgt caacatcttc ttgtctttct
     4921 tttcatactt cttattatat tcttcttttc taaatctact ctatttgatt caattatttt
     4981 ttgtagcatt ttattataag tgaaatcatc taaattatta ttttttatat agagaaaagt
     5041 ttcctcttca catttaataa aacaaattga tagcaaccat gccataccca ttttaacata
     5101 atattcttca ctttgtatat tattacagta ttcaaatatt tctttaatat gtttatcatc
     5161 aacataatat attaaaaaca taactaatgc aaatctaatt tcccatggat tctttgaata
     5221 aacatatttt ttaagtatat catacatatt ttctttatat tttttcatag actttaaatc
     5281 agaacaaaat ccatcgcaaa ctgaccaatt atcgatttta ggtataaatc tttttacata
     5341 gtataaagtt tcttcaaaat tactatttat attgccaatt accatacctt gtagcctaac
     5401 ttcttcatag tatgtatcct gtgctacttc taaaaattcc ttccaattcc cttggcttat
     5461 ttcttttgat aactttctta aattaggaac tctaatacct attatatttt ctgttccagg
     5521 tataagcttt ttattaaaat ctctatattt tatatcttgt atagacatca tatattctaa
     5581 aagttcatta taatccaatt tattccattt tcccctatca aacatcattt aattgctcct
     5641 taattccttt tttcttattg taacataaaa ctctacaaaa aaagcttcta actattagaa
     5701 gctttttcaa aaacataaga aacttttcaa ttttaaattt aaactaaatt aatgctaatc
     5761 tcctacacca aagtctgtat tattgtataa aacttctata tttggatttt catccaagaa
     5821 tttatactta ctaacaaacc atttcactaa ctcttcatct ctttttatac tagaagaatt
     5881 atttataaaa acattcactg tcgctttttc aaaagtattt aggataatct ctatatcttt
     5941 atctatcatc tcttttgttt gtccttttat tccaaccata atacaaggtg aatcaaaata
     6001 ttcttttaca tcttgtggag tcttaaaatt tgcattttta tttaaaatat tatttctaaa
     6061 atcattgtca aaggtttcaa ctccaatttt aaataccaca ggaatttcaa aatactctct
     6121 catttcctta agcctatttt tgtaagacca atgactttct aaaaataatt tttttatatt
     6181 cttctctttt ataatacact ttattttttc taaagtatcc ttaggtaatt caaaacagct
     6241 tccagaattt atgacttcaa gaactccata tttcccagtt acgtttttta aaacctttag
     6301 atttaattta ttcatctctt cttctaaatt agaattatca tctatataat cacaaaaaga
     6361 acactttccc catatacaag gaaatccttt aagcagaaca atttctcttt gatttttatt
     6421 tgttatctca ctatatctat ccattttcta tctattttcc tccagtcaat gataccttta
     6481 aagtttttgg aactgcattt aatcctaaat tcactaaaac aactgctaac agcttatctc
     6541 cataatcagt aaatacttgg gttacaaaca cactaaaaac atcccccagt cctaacactt
     6601 tcaagatttg cactatatat gatgaacctg aagaggtcat acctccaaat aaaaatgctg
     6661 aaattattga acttattata gatgttggaa tggcaaataa aagtactcct agtggagttt
     6721 ttaaacccct taaaaaattc tttttaaatg ctattccagc aagtaatccg gttgatattt
     6781 gaactggtgc aaaatagaga gaatacacat caaacgtaat tccacttatt aaacttccaa
     6841 acacacctgt aacaacagcg tatttaggtc ctaaaagtga agctatcatt atagttccta
     6901 ttgaatctaa gtatacaggt aatcttaatc ccatagctat aaaagcgcct attatattta
     6961 tagaaatacc aaaagctata agtgtaatag tttttacaca tatttttctt ttcacactat
     7021 atcactcctt ccacagagtc tataatgtct tcataacctt taaaaagcct ctttaaaaac
     7081 atgtacataa atttcttctc atcaacttca tttagaacaa ttgcattagg atttttttta
     7141 tagaaattga aagaatccac tatagactga cccatagcta ttccatcttc tacaacttct
     7201 acatatgatt caaatccttt acatatactt ctatctataa agtatgctac tgctagaggg
     7261 tcatttatca cacagccaat tattccctcc tgttcccaat ggaaatcaat ataaaatcta
     7321 gttatttcag ttatatactt tgccatcttc ttatcaagtc tatttataaa ctcaataata
     7381 ttaggagtaa gtacaatttt tctagttaca tctaaaccta ccatgtggat ttttttagat
     7441 aaattcttgt aaacataatc tgctccatgt gggtctaccc aataattaaa ctctgctact
     7501 ggagagcaat ttccatgaat cctaaatgct cctcccatag atacaaactc atcgagattt
     7561 tcaaatgctt tcttatcttt aataagtgct ttagctatat ttgtaagtgg tgcaagtgct
     7621 attattgata ctttttcatg attatataaa gtatttatta taaaatccac tgctccattt
     7681 tttgctttag ctcctacaac cttttgataa aagttttctc caataccatc ttctccatgt
     7741 gtatcttgag ctgttacaag ttttcttttt agtggtgctt cttctcctat atatactgga
     7801 atatttagtg aagaacacat ttgaagtgtt tttagtgcat tttctgcacc tatatttgct
     7861 gggacatttc cacaacatgt ggtaattcca attacttcta gctctggtga gtttaaagcc
     7921 agaagaattg ccaaagaatc atcaattcct gggtcacaat caattattac ttttctcttt
     7981 tccatttttt atcctccttg atttgtggtc gaggagaact acacatcttt tttccacttt
     8041 caaaaaggtg tgtctaaggg tattaattat atactgccac tatataattc cttagttttt
     8101 tatacttgga gttctcgaac aagtacagat gtaaaatcat atttttaggc tacttactat
     8161 aagactctta aaaactatag accgttaaaa aatttttaaa catctgattt aatagctata
     8221 aaataaattt tatagcataa aagaatttat tatattttaa tgtatcttat taaagtttat
     8281 ttagaattat gaaccaactt taataagatg tggcgcattt aaaccgatta ttattataca
     8341 ataatatctt aaatattaca attaataaca ttaatttatt atccataaaa atatattaac
     8401 ttatattatt taataattta ttgacagtat taaaattaaa ttatacaata acagtgtaaa
     8461 ttaacttaac accgttaagc aaagaggtga ttatatgagt atcaaagaaa gacgtaaaaa
     8521 tgaaaaagaa gaaatgaaga aaaagattat ggatgctagc atcgaaatta taaaccaaca
     8581 tggatatgaa aatttatcaa taagaaaaat tgctacaaaa atagaatatt ctccaactac
     8641 aatttatctt tactacaaag acaaagcaga gattatcagc gatatgacca ataaacttta
     8701 taacacagta gaaagtaatg ccattgacat tatgaataat tgctcatcac taccaataga
     8761 taaacaaatc atagaaatta tgactagctt tataaaaact ctttctagtg aaccagagat
     8821 ggcaaaatct atcatgcata gtagaatgaa tattatattt gcatctgaaa atacaaacaa
     8881 tacgcctagc aataatggta taaaaatgct tgataaattc ctttccattg gaattgaaca
     8941 aggaatattc aaaaaaaata tagataaaag ctcatggatg cttataagtg cattgttagg
     9001 attcgtttta tgtgttgttg aaaataaact atattcttta cataattttt ctcaacttat
     9061 agaaaatttc ttagatattt tagtgggagg actttacaat gaaaattcgc aacaaggcta
     9121 ctaataaaaa gaaaaaatat gtaaaaatat ctattgctct attctttgta tttatatgca
     9181 ttattattgt aattgtaagc aacttaaagc cagaaaactt ggtagtaaaa gatatagata
     9241 ttaataatgt taaaaatggc atttatactg gaagtgctga taataacttg gtaaaagcta
     9301 ctgtgtctgt tgaagttaat aatggtaaaa tccaaaatat taatatctta aaacacgacc
     9361 atcttttagg taaacctgct gaaaaaatta caacaagtat tattaaacaa cagtctttag
     9421 atgtggatgc tataacttct gcaacttata gcagtaatac tatacgaaaa gctgtagaaa
     9481 atgctttaag aaagggagaa taattatgaa tactattatt atatattcaa gtaaatatgg
     9541 ttgtacaaaa gactgtgcaa atatactaaa aaataaacta tcagataatg tcactattgt
     9601 tgatattaat aataataata ataaaattga gttatcaaaa tttgataaaa ttattattgg
     9661 aagttctata tatgttggtt cagtttcaaa aaaaatacag gtgctatgta atgataatgt
     9721 agagttatta aacaaaaaac aagttggcat tttcctttgc tgtggctttt ctgaacaagc
     9781 agataaatat ttaaaatcaa attttccatc atccttatta gaaagtgcaa atgctattgg
     9841 aatttttggt agtgaagctc gattagaaaa aatgaaattt ttagataaac ttattatgaa
     9901 ggctgtttca aaaggtaatt atgacagttt tagaatatca caggataata tagataattt
     9961 tttaataaat cttaatagct aaaataaagc taattatttc tatgataaat tcttttattt
    10021 ataagtaata ttgctcaaaa tagtctttaa actttatcaa aattttattt ttcttagtga
    10081 aatctaatga aactagcact taatatatgc tttattaatc ataacaaata tgttttatta
    10141 atcataacaa aaagagagtg tctaatgaac taaaaaattc attagacact ctctttttgt
    10201 atgaaatcaa atatattttt atcatttcaa cagagaaaaa tacacttgtc tctatgtttg
    10261 gcacattctc tttttataat ataatgttta atttaaaact actatttcaa atattgacat
    10321 atacttaaac tatagactta caaaatcaat tcatattagc ttaaaatata atctttgtta
    10381 ttcaaattcc tatacacaac cattatttac tttgatactt taaaacatct tccctatttt
    10441 ttattaaatt ttataataat agtcctatta acgcaaatac tataaaaaga gtttaacttt
    10501 atccattaaa taaatttaaa ttaaaacata tagaaatgga aggagaacaa tttatgataa
    10561 aactaatcgg tatattgata gttgtaattg gatttatact taaaattgac acactattta
    10621 ctgtattatt agcaggagtg gcaacaggaa ttgttgcagg attagatttt aatcaaattt
    10681 ttacaatttt aggagattct tttgtctcaa acagaggtgt atcattattc atattaacat
    10741 tgccagtaat cggtgtatta gaaagatatg gtttaagaca aagagctgtt tctttaatag
    10801 aaaaattaaa aagacttaca acaggaaaag tacttactat atacatgata gctagacaaa
    10861 tagctggtgc tctatcaata agaatgagtg gacatcctca atttgtaaga ccacttgtta
    10921 atcctatggc acaagctgct ggtttaagta atagtgatga actaaaagaa tcggatgaag
    10981 aagccataaa ggcattatct gctgcatcag aaaactatgg aaatttttat ggtcaaaact
    11041 tatttgcagg tagtagtgga gtattactta tagcatctac tctgactcaa tttggataca
    11101 atgtaactgg acttaatatc gtaaaggcta gtactattat ggcagtaata gcacttgtag
    11161 tggctacaat ccagtttacc ttatatgata aaaaattaaa caaaagtcat aaaaaatagt
    11221 tttatttagg aggaataaaa atgcaaaatt ttataaacat atcattggaa atcttttacg
    11281 cactaatggg atttttaatg attgttatag catataagtc ttttactacc ataaataaca
    11341 ataagaaata tggtacatca ttattttgga tacttatatc actgccattc atatttggta
    11401 gattgattcc agccaatata ataggaataa tattaatatt atctagttta ttaacacttt
    11461 ctaaacaagt tgtatttgca aagtatgaag aaccagatga aaattttgga aaagaacaag
    11521 cagacaaact taaaaataaa atatttatac catcattaat attagcattt gctgcagtag
    11581 tagtagctat gagtctttct aattttgata attcttctca gtttgcaata ggtgtgggtt
    11641 caataattgc attaatatca gcccttataa taactaaggc aaaacctgct acatcagttc
    11701 aagatggctc aagattactt caacaaatgg gacctgctag tatgttacca caacttttag
    11761 ttgcattggg agctttattt actcaagccg gagtcggtga agttatttca accatgatat
    11821 ctggtgtagt ccctgctgat agcagattat ttggtgttat agcttatgta ttaggtatgg
    11881 ttatatttac catgataatg ggaaatgcat ttgccgcatt ttcagtcata acggcaggaa
    11941 ttggtattcc ttttgtattg tctcaaggtg gaaatcctgc tataattgga gcattagcat
    12001 taacagcagg atattgtgga actctactta ctccaatggc tgctaatttt aatatagttc
    12061 cagcagcctt gctagaatgt aaaaatgatt atattgttat aaaatatcaa gctccagttg
    12121 ctttggtatt aattatagcc cacatattag taatgtactt tttaggattt taatataatt
    12181 aggaggtaaa ataatgaaaa ttttgttaac aggatttgac ccatttggcg gagaaccaat
    12241 aaacccagct caagaagcag ttgaaagggt caataataat ataaacggtg cagaaatcat
    12301 aaagataact ataccaacag ttatgacaaa atctgttgaa gctatagata aagctattca
    12361 agagcacaat ccagatatag taatatcagt tggacaagct ggaggtagat ttgacattac
    12421 acctgagaga gttgctataa atatagatga ttttagaata aaagataatg agggtaacca
    12481 ggttatagat actattataa aagaagatgg tgaacctgca tatttttcaa aattgcctgt
    12541 aaaagctatg gtaaaacata tgaatgaaaa taaaattcca gctagtgttt caaacactgc
    12601 aggaacattt gtttgcaatc atgtgatgta tggaattctt tatatgatag acaagaaata
    12661 tcctaatata aggggtggat ttatacacat cccatataca acttcacaag taatagataa
    12721 aaaaaataca ccattcatgt ctttagaaga aatagtaaaa ggcttagaat tagctataga
    12781 agcttgtatt atatataaag aagatgtaaa agaaattggt ggtgaaattt cataataaat
    12841 tttttagtat ttttataaat taaaaatact aactattaat ttaaatatag actgtgagta
    12901 gattattcaa acaattttac tcacagtctt tttatggtat ctacagatac aaaattaagg
    12961 tctattcctc tctccccact cacacattgc atctaaaatt ggaatcagag attttcccct
    13021 ttctgataga ctatattcta ccttaggagg tatttgagga tattcagtac gaataatcaa
    13081 gttatctgct tctaattctt ttaatgataa actcaaagtt ttataggaga tagtatttat
    13141 ataacgtttt aatgcattat atcttacaac ttcaaattct acaagacagt aaagaattat
    13201 catcttatat ttaccactaa tcagtgaaag tgtataacta aatcctgttt cacttaaatc
    13261 tgtatttgat atacagtcta tactcatata ttacactctc cttttggtta gtattgaaca
    13321 aaaatgtgcg tacttgtttt tagaataatt tataataaaa ttatagtgta agaataattg
    13381 aaagtcaatt ttataatagg aggtgtattt actaaaaaaa aagttatttt aaataggatt
    13441 ctaagattag acatttctat ataaattgat aatattcatg aaaatcctat tttataaaaa
    13501 ataatgtctc ttagaaaatc tattaaataa aattcattgg aggaaattta taatgaaagt
    13561 tttattgata aacggtagcc caaaccaata tggatgtact tatacagcac taaatgagat
    13621 tactaaagta ttgagtaaac acgatattaa aacagaaata ctgtatttag gtaaagaaac
    13681 aatcccagga tgtattagtt gcgcatcctg ttttgaaact ggaaaatgta ttcgaaatga
    13741 taaagttaat gaattgattg aagatctgga taatattgat ggtattatta ttggttctcc
    13801 tgtttacttt tctagtgcaa ctggtcaatt aacttctttt cttgaccgtc tatttttcat
    13861 agctgggagt cgcatggcta caaaattagg tgcttcagtt gtgtcatgta gacgtggtgg
    13921 tgcaagtgca acatttgacc aactaaataa atatttttca atcagcaata tgcctattgt
    13981 atcatcacag tattggaacc aggtacatgg ctttactcca gaggatgtta tgaaggatga
    14041 agaaggatta cagactatgc gaactctagg agaaaacatg gcatggcttc ttaagtgtat
    14101 atcagcaggt aaaaaagctg gaattaaaga accacaatat gaagaaacaa ttatgactaa
    14161 ttttataaaa taaataaaaa atgtactaat attaattaaa acaaaacact aatttataaa
    14221 taaaataagc ataaatgtag gataaaaagc actaaaacag aggtattgaa ttttatatca
    14281 aacctctgtt ttattattac attaataacc aaaccatgta taacatcata tatttctaaa
    14341 taaattataa tattatattc taatgttatt gaaattacaa taactgaatt tctgctcata
    14401 aatttaaata ttctacaaat ctcatctaaa taacaatttt aatagaccta aaacaatcct
    14461 catactgttc attttgtatt aaaaatatca cttacaatca aaaaccattt attaagtata
    14521 ttcctaagat atacaatata tatacatgga ttggatagta taaataaaat aaatacttca
    14581 tactcttccc tttttctcca ttgtaaagta acattaaagg tagagaagca atcatcatcc
    14641 attggtaatt ttgtaaaata gaatttttta aactataatc tccatttgac attgttgtaa
    14701 atataaatat acacattaaa acataacaaa tactcaaact gaacttttta cctctacaca
    14761 aataaaaaat aatcccaagt ataatccaaa ttggtccacc ttctactaaa aatggtactg
    14821 gtaccaaaat cataaataaa gcaataactc tgttagtgac tttaaaaagt aaagctactg
    14881 taaatattcc taaaattata ggaatgatta tcatcaataa tcctataata aaatatttaa
    14941 tttctttctc ttttctaaat ttctttatga actctattgc ttgtaaataa atcgctatta
    15001 aaaataatgt tgaaaaagtg ttatttataa ttatagctcc ttctggatgt gaaaatacat
    15061 tattaattat ttgatttcct atagccataa taacagacca aatatacaat ctacttatat
    15121 atttttttct gttactagtg tgataaaatc cttcaacagc catgaatata aacagtggtg
    15181 ctgatattct gcctacccaa tgaaaccaaa ttggtatttg cattgtttgt ggcataaatg
    15241 ctgctatatg gtcaaatgtc ataaatatta atgctaaaat cttaatagta aacccactta
    15301 tacctttttt aaaaaaatcc atatcatatc tcctaatttt gttttatctc tataataatt
    15361 tttgttccta ttccaatttc actacttaca taaattttac ctttatgaat ctctattatt
    15421 tctttagaaa tagccatacc aagacctgaa cctttatgca tctcacctgt attagtacct
    15481 ctataatatt ttttaaaaat atgtttcaaa tcacttttgc ttattccaat tccatcatct
    15541 tttattataa tttctatatt atcttttttt acaatttcta cacttatcaa agttccttcg
    15601 ctattatgaa ctattgaatt aaatataaga ttggttatag cccttctgaa taagattgaa
    15661 tctacatata cttcaattaa atcttcatta ctttcaaatt caatattcct atttttatat
    15721 ctattatcat ttaaaatatc aattattata tctctgacta aactgactaa atttattttt
    15781 ttcttgtcca gaactatagt attatttttt aatcgagtag ataaatttaa atcatcaact
    15841 aaatctttta tatacttaga cttattatat ataatttctg tatactcttg tatttcatct
    15901 tcttcaaact cataatcttt gtcattaatt atctctgcat acccttgtat agatgctaga
    15961 ggtgtcttta tatcatgtgt tatatttgcc aaccattcct ctctcaactc ttcattttct
    16021 tttctctcaa cttcatttac tcttaaagta tcagctaaca tatttatatt tttaaaaact
    16081 tcttcatata tacctctgtc cttaagatat aaactataat ttccattagc catagtctca
    16141 atactccata gtacgcttga aattggtttt gtaagttttc tactaaataa atatcctaca
    16201 cctaaagcta ccacgctatc aattactagt gttataatga taactttatt taaagttttt
    16261 ttaatgttat tttgagaata ggttaaaatt attcttgata tatctctact aggtatacct
    16321 actattatgt ttagatgttt tttgtttaaa tatttttcta atacaaaatt aacatacttt
    16381 gtttctatat acttgtaatt attcaccatt tgaaagggtg tatatttttt gggagtatcc
    16441 tttggcacat ttactccata aacttcctct cctaaatcat ttaaaacctg tatccaggaa
    16501 tttgattttt ctaatttttc agctccaatc ttagatagtt taaattctcc atctttttca
    16561 tataaatctt tttcaaaaga acgtgcaaat tcttcaggat tatttccaga atctacaact
    16621 ttaaaaaaac tattagttga aatcacatac aatatagata tgatatttat aattactact
    16681 gatatagcta caaacacaat tgtaaatata aaatttctag taatcttcca cttcatattc
    16741 taatcttcct ttactgctaa tttatatcca agacctttta ttgtcagaat atattcaggc
    16801 tttgaaggat tatcctctaa tttttcccta atttttctta tatgaaccat tatagtattg
    16861 tcaaatccaa cataatcttc tccccacact ttatcccata gtttttcttt acttataatt
    16921 tgattttgat tatgtgcaaa taaagatagg atttttagtt cctttgcaga cagtcctata
    16981 gattttccat cttttataag ttctgcccta ctttcattta atatatatgg gccaaatgat
    17041 ataatttttt cttcattatt atttagattt ttactttcgt tttgagcatc actaaaataa
    17101 cttaatcttc tcaaatgcgc tttaactcta aaagccactt ccttagggct aaaaggtttt
    17161 gtaacataat catctcctcc aatcgctagt ccaagtaact tatctaattc ttcggtttta
    17221 gctgataaaa atattattgg agaattggag gtttttctta tttctttaca aatatcataa
    17281 ccttcgccat ctggtaacat aatatccaat acaatcaaat caggattaat acttttaaat
    17341 aattctaatc cttctttttt agtttttgca gtatatatat tattaaggcc ctcttttttt
    17401 aggacagttt ctaaaagctt caatatttct acttcatcat ctattattaa aacacgattt
    17461 tccataaaca acctcctgta ataatttaca tatatattag catatatttt atctaaaatt
    17521 catttatctt atttttaatt aaactaaaat ataaataaaa ttactattat ttttagttaa
    17581 aaccttttgc ttgatgtaaa attttcatat gttattttag ttaacactat cattaatata
    17641 attgagatta ctatagaaaa aactatcaaa atacttggta cattatactt aaccatttgt
    17701 tggctcatca taatccaatt tggagtcact gacataaatt ttatagcttt atctggtata
    17761 aaacctacac taaagtatgg aattgccatt atcaatccac ctagtataaa tggaattaca
    17821 gccgatttaa atatagatga aagtgttaag cctatttgtg caaatataaa agaagcaaaa
    17881 atagtagtca atataacaat tatatacatt tgtattactg taagatttaa attactattt
    17941 ccaaagtacc ataagttatg aatcgattca tttaaatttt taaaatcaaa aaaactaaaa
    18001 cctctaattc ctactaacaa tataaaagaa atattaataa tagataaaat taatccatta
    18061 gctactagtt ttgataatac tactgatttt tttccctttt tagttgtatt tattaaacct
    18121 gtcatattag aatcatattc tttaatgtaa tttccagaag ttattataaa cattatcaaa
    18181 cctattagag gtattgagcc ttctatcttg taaaatatat ctgcataagt atctaaaaca
    18241 tcaaactttc taatcagaaa aaatccaaaa aatattaaaa acagccaaat tatccaaaca
    18301 ctctttgatt ttaatatttt tatcatttca gctttaacta aatacatcat ttctactaca
    18361 cctcccttgc atcttctaaa tcaaaataaa acatgtatac atcttcgaat tttgcttgaa
    18421 ctggttgtat attatcctcg cttggaatat cctctgaaac aattctaagt actactgaat
    18481 caatatcact ttgaatactt acaaccttat atttagattg tatatcatca acttcacatt
    18541 catttttaac ttgaacatta tatactttat tttccatgtg acttaatatg tctttgtgtg
    18601 ttcctttcat taggagtttg ccatctttaa tcattattgt ttcctttgca actgattcta
    18661 tatcggaaat tatatgagta gaaagtatta ttatcttatc tctagacatt tttgctatta
    18721 aattcctaaa tctagctctt tcttgtgggt caagtcctgc tgttggctca tcaaggacta
    18781 ttatcttagg gtcgtttagt aaaacttgtg ctatcccaac tctcctcttc atacctcctg
    18841 aaaattttcc tatagactta tttctcacat cataaagacc tactaaatct aataattctc
    18901 ctatcttact atttaaatat tttcccctta aaccttttaa agctccaata tactgtaaaa
    18961 attcttttgc tgtaaaattt ttataaactc caaattcttg tggcaaatat cctaaaatat
    19021 ttctataatc agcatccatt ttaaatatat cctcaccatt ataaagtatc tgaccttcat
    19081 caggttttat caacgttgtt atttgtttca ttaatgtaga ttttcctgct ccatttggtc
    19141 ctagaagacc ataaactcca ttttccagag ttactgtaat cttgtcgttt gctacttttt
    19201 taccatatat ctttgaaaga ttttctattt ctaatttatt catcttaact ccccttttat
    19261 ctacttatat aatctgattt tattactaaa aaacataatg acatagcacc tatcaatatt
    19321 gccatcaaaa gtcctattgc tatattgaca atgtctatat tcactttaaa tatcataata
    19381 tcacctagat aatttcctat tattttatta aaaccaaata tcaaatcata ataatttaca
    19441 tttgatacaa ctgaaagtaa ttttttaggt aatatatgga ttaccgacat tatcttagtt
    19501 atagatataa taaatataaa aaaacttatt gaagatattg aagacttact tataaaagac
    19561 ccaaatatgg ctatcatggc ccatccttca aacatcaaaa gctctatgcc tatatttaat
    19621 attacatact ttgttatact taaattagtt ttcaatgcaa ctacattgtc tactattcta
    19681 aaagattcta agtctccatt aacaggtact ccttgttgca tacaagttat aataaatatt
    19741 cccattaaat aaacagaata aattactaat ggaactaata aagcaacaaa tattttagac
    19801 tttaatgctt ttttcttctc ttttgatgat agtatgatat cttttaccga agtaacgact
    19861 tcatctacat aaatattgga tataattata gatataatga ttaacattcc tacatttata
    19921 agtgaatttg tgactctata gttaaatact ttccaaaaac taatatcctt atattcattc
    19981 acacttagat tatcaagttt tttctttgac attaattcaa tttttttaga aaattcacct
    20041 gtatctgttt gatttgaaaa ctgctttaaa acattaatct tattttgaaa tttctcatta
    20101 cctacgtcga taccacttct cgtatcttct atacgtcctt tttctttatc tatataataa
    20161 ctttctgttt ctaaaacagg cgtaataaag atactaatca ttagtattaa cactaatatc
    20221 cctagagata taaacatact cttagattta gccaattttt ttatttccca atttaccaat
    20281 atatttctcc tcctaaatca ttttaattac acatatatat taaaacttaa atcttatata
    20341 aatataatac taaccttaaa taaagcttaa attattttat aatttatagg tgtgtttatt
    20401 attggagtta catttattat ttttctggga aattatacat tttcaattat ttttattatt
    20461 tttgatattt ttattgattg gaaattttat tgttgttcaa attgtagtta ttctttaaat
    20521 ttaaagatgg agttttttaa gaatagaacc tactgtcctt attatggaaa gattattaaa
    20581 tagttgcata taactttatt tatcttgaat cttattttta aaaacaatta taattttaga
    20641 atattttcta ttctcaaaca agcatgttga ataatggagc atcttctagt attatatact
    20701 tgtagaataa aactaaattg gtaaaactgg gaatatttaa ccttaaggag ttaagttata
    20761 gaaactagta ctaaaaaatt ttataaatta tgttaattga ctaatagaat gcgacttttt
    20821 atttttacta aattttttag ggggatggat aatatgttta ttggagaaat agacaaatgt
    20881 acacatatac taactgctta catcagtagt tcatatgatt attgtaattt tttagataca
    20941 cagttagatg attttatatc agaatatgga gaaacggtag ttgaaacctg cttacatcaa
    21001 gtcttattat tagtaagtag atacaattaa aactaaattt ttctagaaaa acctttttag
    21061 atttatctac tctaataaat atataatttt ttgtacttta taagtaacta taacttctgc
    21121 taaagtaaag atgtattggg agtacatctt tactttccca ttatagaagt taaaagcata
    21181 caccataaaa ttaatcatcc aatacaaatt atatatttcc tatgtctaac ttaatttaca
    21241 agaatcattc tatatgcata ttctgcaatc cactaaacat ggctctaaac ctttactatt
    21301 ttatacattc tctataaatc atacaaatca tatataaaat ccccctaata tgtttaaacc
    21361 acctataaaa taacaccaat atttagaata ttcaatatca actgcataac tggtcaataa
    21421 aaacgtataa ctagacaaat ttcaatattt atataaataa tacaagaaaa acaagcattt
    21481 tatttatata agttttttat tttttg
//
LOCUS       DAMJVK010000067        20894 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.068, whole genome shotgun sequence.
ACCESSION   DAMJVK010000067 DAMJVK010000000
VERSION     DAMJVK010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20894
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.068"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            299..688
                     /locus_tag="PC414_002545"
     CDS             299..688
                     /locus_tag="PC414_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2464933.1"
                     /translation="MKINKLPHAELKLMKYIWGVDDVLASRDIIEDMKLKYDWKKSTT
                     LTFLKNLVDKGFLTTDKVDRCTHYTIAIKEKDYLKVETKSFFSFMHNNSFKSFISALH
                     DDEVLDSKSLDKLEEYFKNLKEEDIDD"
     gene            1008..1517
                     /gene="vanZ1"
                     /locus_tag="PC414_002546"
     CDS             1008..1517
                     /gene="vanZ1"
                     /locus_tag="PC414_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ1"
                     /protein_id="HDF2464934.1"
                     /translation="MKSRKHNITKGLFIVYIIILTWIILFKLQFDISSLETMNLRSIN
                     LIPFAGSLIINNRVDISEIILNVAIFVPFGIYVCMLKEEWSFIKKVIPIFITSLVFET
                     LQYIFALGASDITDLIGNTLGGIIGIAVFMLLSKIFKNNTIKIINVLALIVTIIVVLF
                     LGLVIFANL"
     gene            complement(1619..1813)
                     /locus_tag="PC414_002547"
     CDS             complement(1619..1813)
                     /locus_tag="PC414_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase"
                     /protein_id="HDF2464935.1"
                     /translation="MSVIVMCSRCGKEIDKYRNKYITYYEKNTNAEIKVCINCALDLL
                     EKTRENQELNIQEFQDTIIL"
     gene            complement(2329..2721)
                     /locus_tag="PC414_002548"
     CDS             complement(2329..2721)
                     /locus_tag="PC414_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2464936.1"
                     /translation="MAALKSFEKPLTPEEEIEYLTKFKIENDKSAKDTLIERNMRLVA
                     YIAKKYNNSTEDQDDLISIGTIGLIKAIETYNIDKGTRLATYASRCIENEILMNIRSN
                     KKNKTQVSLQDPIGTDKEGNEINTTLYM"
     gene            3288..3650
                     /locus_tag="PC414_002549"
     CDS             3288..3650
                     /locus_tag="PC414_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464937.1"
                     /translation="MKIRKSGFISIECIISIAILYVAVYLVSTSLYNCYSFISRNISD
                     REMLSTAKKYIEDEKYRIQNSKYELIEDKIEKNYINGYEINSKIEQILDYYQCYEINI
                     EIKNEFKKLRFNSYVTRK"
     gene            3634..4359
                     /locus_tag="PC414_002550"
     CDS             3634..4359
                     /locus_tag="PC414_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464938.1"
                     /translation="MLLENKGSILIITLIIFSIISTVCIMCIGLIYTHNNMFELGYKD
                     IQLKEISLSGIEISVSNVLSCVEEAIDVSNNEDEFREYFLGNNMIECIKKIKDTSYSS
                     LEELSLKVENNTIYDRGDFFEFDIESNVREKEFLKAIKVRVKIKNPWIGIDIEDYMDK
                     SNNNKIDDKTFNNYKASENNEILNQFKNESENIRNQEVGGFTEEDEEDEEYEEELKQN
                     IIENIKKNFDEKNLVQVYKYRGV"
     gene            4379..4798
                     /locus_tag="PC414_002551"
     CDS             4379..4798
                     /locus_tag="PC414_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464939.1"
                     /translation="MTVIELIVSISIILLIVTLSFPKDNLENHKINLFARQLCSDIRY
                     VRRINMFSNYNTYIYYINQGKHEGYVLRKDGKNIKSIMLPKNAKIARSIEIIKFKSDG
                     SPQKASTIEIYNNRLRKTITITPVSGRVLLKEGKYET"
     gene            4788..5312
                     /locus_tag="PC414_002552"
     CDS             4788..5312
                     /locus_tag="PC414_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464940.1"
                     /translation="MKHKYGYLLLESVVSLSSMVIIILVLYSIFLSTINLKLKVEDKI
                     ELQQQSLEIIKSMEGIISNSMGIMNVSNYEETFKKTTSIKCRYLDENNNEEGISNKEI
                     ILNERRNKLFVNSLNGESSQAGGYEIGDYVDEMYVSITNNGQYVNIKLKLSKRSQKYE
                     TDFKIKVWNFSESI"
     gene            5624..6181
                     /gene="efp"
                     /locus_tag="PC414_002553"
     CDS             5624..6181
                     /gene="efp"
                     /locus_tag="PC414_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDF2464941.1"
                     /translation="MVSAGDFRKGVTFEKDGQPCLVVDFQHVKPGKGAAFVRTKYKNL
                     KTGAIREESFNPSEKFPKAVIDTRQMQYLYNDGELYYFMDQENFEQIPLNYEQVEDAI
                     KFLKENEVATIRFYQGQPFQVEAPNFAELEVTDTEPGIKGDTASNVTKAATVETGAVV
                     QVPLFINTGDKVKIDTRTGEYLSRV"
     gene            6273..6728
                     /locus_tag="PC414_002554"
     CDS             6273..6728
                     /locus_tag="PC414_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF2464942.1"
                     /translation="MKHLYEEVAYLKGLAEGLEISAESKEGKMIHKIVDALEVFADAI
                     VTLDEEQEELQDFVESIDEDLADLEEDIADMEEDLYEEDDDEDDEDFSYIEMECPNCG
                     ELVEIDEDLLYDDEVDVVCPDCKAVILSSEDDCDDDCTCGGCSSCDDRE"
     gene            6891..7601
                     /locus_tag="PC414_002555"
     CDS             6891..7601
                     /locus_tag="PC414_002555"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HDF2464943.1"
                     /translation="MKISKKLLDNIQRFENVINYKFKNKEYILEALTHSSYSNENKKY
                     NFNERLEFLGDSVLGIVISDYLFNEEANLPEGELTKLRANIVCEDSLSEVANDINLGI
                     HMLLGRGEEATGGRHRTSILADAFEAVIAAIYLDGGFESARQFILHHMENIIYDSRKG
                     NIFRDYKTHLQEVLQGNGENNIWYRLIEEKGPDHNKRFVMEVGINDDVLGIGEGKSKK
                     EAEQLAAKIALKKKLWEK"
     gene            7655..8725
                     /locus_tag="PC414_002556"
     CDS             7655..8725
                     /locus_tag="PC414_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2464944.1"
                     /translation="MKKRIIPIFVPHKGCPHDCIFCNQKKITGVSTDVTSEDARNIIE
                     ECLETIDKDADVEIAFFGGSFTAIDIDIQKSLLSVAKEYVEKGLVKDIRMSTRPDCID
                     EDILSMLKEYKTSIIELGVQSLDEKVLLDSIRGHQSEIVYKSSKMIKNSGIKLGLQMM
                     IGLPADTEEKCIFTAKKFVELKPDCVRVYPTLVVKDTGLEKLMEQNKYNPFTLEESVN
                     IVKKVLVLFYVNNINVIRVGLQATDDIQIGKAVLAGPYHPAFRELVEADMIKDYLEFV
                     ILQNKNIKQMLVKSNKKNISKIIGNKKTNVKYMEEKFGVLLKTQESDLDINQLEIVLD
                     GKSLISANMRDIHRKLYDIYDL"
     gene            8753..12307
                     /gene="smc"
                     /locus_tag="PC414_002557"
     CDS             8753..12307
                     /gene="smc"
                     /locus_tag="PC414_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HDF2464945.1"
                     /translation="MYLKRLELKGFKSFPVKTDIIFKEGITAIVGPNGSGKSNISDAV
                     RWVLGEQSIKSLRGDKLEDVIFAGTDTKKPMNYCEVALTIDNSENQLELDFTEVTIRR
                     RAYRNGESEFFLNNKSCRLKDIKEVFLDTGIGKDGYSIIEQGKVDEILSNNPLSRRKV
                     FDEACGISKYRYKKQEAERNLSNTKENLERIDDVYIEIENQLKPLFNQQTKAKKYLEI
                     SEKLKTLEVNSFIREIEGIEKELSEVNEHRKVIEKELNEKEEQKNVVEKKQEDINKEV
                     EVLQDVIEKSVDYINSIKGVISKKESQINLIKERIRNFTNEISRKNLEIKDIKEKLNE
                     NKQYIKELESNKLSGSEELSTLQENIKVLEGSKDKQKIKLESLNNEIELLKESIIDIL
                     NKKQEFSNKLSTLNANKENMNIRDENINSEITELNKNIEIKSSELDTINKEFNMQNEN
                     LKNVNNRHKELSINLQDSISEHNKLEDEIQKSKYNLNGYNSKLNVYIDMENHYEGFNR
                     GVKEVLKNKNLKGVHGALGQIINVPEKYEKSIEAALGAYMQNIITDNEFSAKFAINYL
                     KQNNLGRVTFLPLNIIKSNKISLGNLKANTKFIGIASDLITFDEKYRNIIENILGRTI
                     LINNIDEGIKFAKETGHRFKIVTLDGEILNPGGSLTGGSLKTNGNILSRKRYINEYTE
                     KISNIKNEISHLELKRESLDKDVKNIKNEIDSHESKIKDLEKSIIIKSTSIKNVESEI
                     ESLKGSITKLENEKNDLNSNLNYTLEKSDDVRKDMEELDDLYNKNKEKIDALNEEIKR
                     YNDLYDKEKSEFDELNLSLVKKTEVYNSIVRDIKRISGENCELEEKNKQLEESLNYEE
                     HEIIKLQDSILTEEKEKENLTKQLGDSNRNLETRKIAKDDLKNSFDEINKELKTIDRQ
                     HIELKESLFKVGGRLERLKTSQDTYINKLFEQYDMTLVQALEIKDEDLDIDRKFLESL
                     KREIRSLGNINIDSIKEYEEIKERYDFYSEQKQDLEESMEEIEKLIHTLEENMKSEFE
                     IKFEEISKNFKYVYKRLFGGGCGELTILDKENLLESDILITAQPPGKKMKNLNLLSGG
                     EKALTAISILFAILITKPTPFCILDEIEAPLDDANIFRFGEFLKDLSKETQFISVTHR
                     RGTMEAADYIYGVTMQEKAISKVISLKLKEAQEITDII"
     gene            12334..13614
                     /gene="ftsY"
                     /locus_tag="PC414_002558"
     CDS             12334..13614
                     /gene="ftsY"
                     /locus_tag="PC414_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HDF2464946.1"
                     /translation="MLKKLFGFGKDKEKEIEKKDAEEEIEVEDSVDNLENLEETIFSG
                     LEEEVIDKVEDIEEKNEESVNNEGTEEIENFEKIEVEMDSNGKEVENISNNDSQEEII
                     EELENYDEIESKEIEDKEDKKVNLFERLKQGLTKAKQGITDRIDEVLKSYTKIDEELL
                     EDLEEILITADVGVNTTMDIIERLRDKIKQKGITEPIKVREELKSIVEDILTNENSTL
                     DIEPAPCIILMVGVNGVGKTTTIGKLANRYKKDGKKVLLAAADTFRAAATEQLEIWAN
                     RTNVDIIKHQEGADPGAVVFDAIKAAKARKTDVLICDTAGRLHNKANLMNELGKVFKI
                     VDREFPEAKREVLLVVDATTGQNAVVQAKTFKEVADITGIVLTKLDGTAKGGVVLAVK
                     SEVDVPVKLIGVGESVEDLQDFNAKSFSDALFGN"
     gene            13717..14085
                     /locus_tag="PC414_002559"
     CDS             13717..14085
                     /locus_tag="PC414_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HDF2464947.1"
                     /translation="MNIEKMVEIGLLFEQYKELLTDKQKEIVALYYEEDYSLGEISEN
                     LNVSRQGVYDTLKRSEKILRDYEEKLHLVSKIQEQEKNIKIIKDKIIDIKEDLLHNRD
                     CANLIPKLENIEDVCREMIK"
     gene            14082..15434
                     /gene="ffh"
                     /locus_tag="PC414_002560"
     CDS             14082..15434
                     /gene="ffh"
                     /locus_tag="PC414_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HDF2464948.1"
                     /translation="MIFEGLSDKLQGAFSKLKSKGKLTEVDVKNAMREVKLALLEADV
                     NFKVVKDFIKKVQERCVGQEVMQSLTPAQHVIKIVNEELTSLMGDVQSKVMISSKPPT
                     ILMMVGLQGAGKTTTAGKLGGYFKKQGKKPLLVACDIYRPAAIKQLQVVGEKLDIPVF
                     NMGDKENPINIAKAGLSHAIKNANDLVIIDTAGRLHIDEVLMDELKSIKSEVKPHEIL
                     LVVDSMTGQDAVNVAESFNEALGVDGVVLTKLDGDTRGGAALSIRAVTQKPIKFMGMG
                     EKLDDIEPFHPDRMASRILGMGDVLSLIEKAQENIDLEKAKELEQKIKKQELDFEDFL
                     QQMEQIQNMGPLDKILGMIPGMGNVKDQLGDVDLNGKEMKRTKAIIQSMTTYERRNPG
                     VLNASRKKRIAKGSGTSVQDVNRLIKQLNEMKKMMKMFAGSQKSMKKRGGLGGLPFFK
                     "
     gene            15472..15744
                     /gene="rpsP"
                     /locus_tag="PC414_002561"
     CDS             15472..15744
                     /gene="rpsP"
                     /locus_tag="PC414_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HDF2464949.1"
                     /translation="MAVKIRLKRMGANKKPFYRIVVADSRAPRDGKFIEEIGYYNPIS
                     EPKQVRINDEKAIKWLATGAQPTEVVKKLLVKNGVIEKFEASKQAK"
     gene            15809..16036
                     /locus_tag="PC414_002562"
     CDS             15809..16036
                     /locus_tag="PC414_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HDF2464950.1"
                     /translation="MKELVVDIAKALVDNPDSVVVEEFEDNDGIVLKLTVAQEDMGKV
                     IGKQGRIAKAIRTVVRSVANRENIKVSLEIV"
     gene            16144..16659
                     /gene="rimM"
                     /locus_tag="PC414_002563"
     CDS             16144..16659
                     /gene="rimM"
                     /locus_tag="PC414_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA processing protein RimM"
                     /protein_id="HDF2464951.1"
                     /translation="MKEKLTHFKVGQIVNTQGLKGEVRVYPLTDDIERFDELKDFYLG
                     KDLNNKLAVEKVRYKGSMVIMKIKGIDSIEKAEKLKNKFMYVAREDSRELEEGEFFIA
                     DMIGMEVLTVDGKYVGILEDVLQYSANDVYVVKGEEDKEFMIPAIKKFVPTIDIDERK
                     MIIDPIKGMID"
     gene            16668..17363
                     /gene="trmD"
                     /locus_tag="PC414_002564"
     CDS             16668..17363
                     /gene="trmD"
                     /locus_tag="PC414_002564"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDF2464952.1"
                     /translation="MRFHIMTLFPEIFNSYMDESIMKRAVEKGIIEVHIYNIRDFSNN
                     KHKKVDDYPFGGGAGMVMTPQPIYDTYKHIITTHNINNPSVIYLTPKGKVYNQSMAKQ
                     MSLKEDIILLCGHYEGIDERIIDLIVTDEISIGDYVLTGGELPALIMIDSISRLIPGV
                     LNQEESFEEESFKDNLLEYPHYTRPRDFEGLKVPEVLLSGNHKKIDEWRREESIRITK
                     ERRFDLYKKSNEK"
     gene            17548..17898
                     /gene="rplS"
                     /locus_tag="PC414_002565"
     CDS             17548..17898
                     /gene="rplS"
                     /locus_tag="PC414_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HDF2464953.1"
                     /translation="MNEMLRAIEQEQLKNEVPNFGPGDTVKVHVRIIEGKRERIQVFE
                     GVVLKRQGGGARETFTVRKMSFNVGVERTFPVHSPKIEKIEVTRKGKVRRAKLNYLRG
                     RVGKAAKIKEARNK"
     gene            18219..19115
                     /gene="ylqF"
                     /locus_tag="PC414_002566"
     CDS             18219..19115
                     /gene="ylqF"
                     /locus_tag="PC414_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HDF2464954.1"
                     /translation="MSNEYEEYLMNDNLHINWYPGHMKKTKELVKNNLKLIDVVIELL
                     DARIPFSSKNPDIDRLVGDKPRVVVLNKSDMADRDKLNQWIEYYKKINIKAIPVDTIK
                     GVGINKIIEECKNVTREKMSSLKDKGRKERAIRIMIVGVPNVGKSSLINKLTGRKSTQ
                     TGDKPGVTKGKQWVRLKGNLELLDTPGILWPKFEDQEVALNLAFSRAIKDEILDTETL
                     ALRLIEKLMKIEPEKLKARYKLDCLGETPIETMDMIGHKRGFITGKKELDYTRIATTV
                     LNEFRDGKIGNITLEVPENVKR"
     gene            19504..20796
                     /gene="brnQ"
                     /locus_tag="PC414_002567"
     CDS             19504..20796
                     /gene="brnQ"
                     /locus_tag="PC414_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDF2464955.1"
                     /translation="MGKTKDVIVFGFALFAMFFGAGNLIFPPYLGIITGPEWLIAFLG
                     FTFADAGLALLAVMATAKFDGNVVEMFKRCGMKLGILIGCADILCIGPFLAIPRTGAT
                     TYEMGIMPLFGTSIPVLLFCILFFAISYVLTIRPSKVVDIVGQFLTPALLIALAFIII
                     KGIISPLGDIVDKPMIPNVFAEGIGQGYQTMDAFAAIALASVLIVSLNDKGYSTISDK
                     LKMIGKAGVLACGGLALVYGGLCFLGATVSTMYGTDAVQSQVIVNITEGLLGNVGKAI
                     LAVVVSLACLTTSIGLTSATGQYFSRLTKGKLSYEKIVLAVSVFSAVVASFGVGTIIK
                     IASPILSIVYPPSIVLIILAFFNEKIKNDNVYKGAVYMSLFVSILTVISSYGVAVPVV
                     NSLPLNSLGFNWVVPVIIAGIIGNFIPSKSQSNTLGTN"
BASE COUNT         8778 a         1955 c         3753 g         6408 t
ORIGIN      
        1 aagtatatga aataaatatc ctgtatttaa gcttatagat tataatgaaa tataatacat
       61 gttgcaataa taaaacgaac taaaattatc acactaaagc ataaaacata ctctaatcat
      121 gttagataaa tttttgaata tataatccaa aagtactctc taaagtagag tacttctttt
      181 ttattaatta tttttatata gaataaattg gaatttatat ttattccaat ctaattgatt
      241 acatacactt attgttataa agtttagtta tacataaatt ggtaagaggt gtaaactaat
      301 gaagattaat aaattaccac atgcagaact aaaactaatg aagtatattt ggggagtaga
      361 tgatgtatta gcctcaagag atatcataga agacatgaag ttgaaatacg attggaaaaa
      421 gagcacaaca ttgacatttt taaaaaattt agtagataag ggctttttaa ctactgataa
      481 ggtagataga tgtacgcatt atactatagc tataaaagag aaagattact taaaagttga
      541 gacaaagagt ttttttagtt ttatgcataa caattcattt aagagtttta tttcagcact
      601 acatgatgat gaagttttag atagtaagag cttggacaaa ttagaagaat actttaaaaa
      661 tttgaaagaa gaagatattg atgactaata aagctatcag tatcttttta gtttgagaca
      721 attgagagta gcgtttggat ttaatgttag ttttatgtga tggtagaaca ttatataaag
      781 aaaagtgatt atttccttag tgtttgatag taaaaaatat agagaataat accaattaaa
      841 atttaaagat atatgaaata ctacaatagc ttaaatcatg taaatatgat aaagatattg
      901 aaattataaa aggaatataa ttttagaaga aatagttgac agataaatta ccttactata
      961 ttattgtatt aataaaataa tacaataata tagtaaggag gtaataaatg aaatctagaa
     1021 aacataatat tacaaaagga ttatttatag tttacataat tattttaaca tggataatac
     1081 tatttaagtt gcaatttgat atctcatctc ttgaaaccat gaatcttaga agcataaact
     1141 taataccttt tgctgggtca cttataatta ataatagagt agatatatca gagattatat
     1201 taaatgtggc tatatttgta ccatttggga tatatgtgtg tatgctaaaa gaagaatgga
     1261 gctttataaa aaaagttata cctattttta taactagttt ggtatttgaa acacttcaat
     1321 atatatttgc actaggtgca agtgatataa cggatttgat aggaaacaca ctaggaggaa
     1381 tcataggaat agctgttttt atgctattat cgaaaatatt taagaataat accataaaaa
     1441 ttatcaatgt attggcattg attgtgacaa ttattgttgt attattttta ggattagtta
     1501 tttttgcaaa cttatagaaa taagtatata tgtttagctt aaaatagaac gtaagataca
     1561 gttacatata aaattattta cacataaaaa gcacctatac aggtgctttc ctaaattatt
     1621 aaagaattat tgtatcttga aactcttgta tgttcaattc ttgattttct cttgtttttt
     1681 ctaataaatc taacgcacaa tttatacaaa ctttaatttc tgcattggta tttttctcat
     1741 aataagttat atatttattt ctatatttat ctatttcttt tccacaacga ctacacatta
     1801 caattacgct catatcttag ttcctccatc tctaattatt cttaatatat tcaatagtat
     1861 cacttttctg aaaataatga ttgttaaaac tgtatcaaat ttagatttac tttttattat
     1921 aatgcatttt aataaaaaaa taaaggttat gggtaaaata tttatagaaa aaagtatatt
     1981 attgaataga ttataaaatt ttattatttt ttagcagtgt taaatacaca ttaatatcta
     2041 taaagtgaca aaataaaaaa tactatttaa atagatatta aaaaattgat accaagtatt
     2101 tatttgctat atgaataaca atttatttag gaactttact ttaacactgt ataataataa
     2161 agtaattgct aaaaacgttt gatgatttgg atgtattttt agaaattaga aaaatatata
     2221 cttactgact aatgataatc ttatatgtat tttagtttga gtgatttaag aaaagtgttt
     2281 tgaatgggga ttattttata gagtaaataa atatataata gtagaaaatt acatataaag
     2341 agtagtgttt atttcattac cttctttatc tgttccgata gggtcttgta gagatacttg
     2401 agttttattt tttttgttag atctaatatt cattaaaatc tcattttcaa tacatctaga
     2461 agcatatgtt gctaatcgag ttcctttatc tatgttataa gtttctatag ctttaattaa
     2521 tcctattgta cctatggata ttaaatcatc ctgatcttcc gttgaattat tatatttctt
     2581 tgctatataa gctactaatc tcatgtttct ttcaattaat gtatcttttg cacttttatc
     2641 gttttctatt ttaaatttag ttaaatactc aatctcttct tcaggggtta agggtttttc
     2701 aaaagattta agagctgcca ataggtcacc cccttttcaa cggtcacttt aaattatatg
     2761 aaggagactt atccaagttg actgttatct aataaaataa tatgcatatt tttagaattt
     2821 aaaacatata tttgcaaact tttgaatgga atatgacata aagagtggga atttaaattt
     2881 aagtttttgt tgtatatata tgaaataagg cgaagaaatt attgatttat aaagtataaa
     2941 ttggaattga ggtgtcaata aatataaaag gaaatagtat ttatatatgt tttggtgttg
     3001 aagtaaaaga ttaatgcaaa gcattagagt ctataaaaat atgaatatag gaggtgtaat
     3061 tttacaatac ttaaaaaagt aaatgtaatt aattgtttgt ataaagttga ttacaagcaa
     3121 aagtatcagt catagttaat actgttaagg gtgaaggtgg tgtgttaatt tatatatgta
     3181 tgggaagaaa ttttgattac aatgaaaaaa cttacaaaag atatttatga aaaaacttat
     3241 agttaaatgt ttatatataa aatattattt aggggttgtt tttattattg aaaataagaa
     3301 agagtggttt tatatcaatt gaatgtataa taagtattgc tatattatat gtggctgttt
     3361 atttagtttc tacatcattg tataattgtt atagttttat cagtagaaat atatctgaca
     3421 gagaaatgtt aagtacagca aaaaaatata tagaagatga gaagtataga atacaaaata
     3481 gtaagtatga gttaattgaa gataagatag aaaaaaatta cataaatgga tatgaaatta
     3541 acagtaaaat agagcaaatt ttagattatt atcaatgcta tgaaataaat atagagataa
     3601 aaaatgaatt taaaaaactg aggtttaata gctatgttac tagaaaataa aggctctata
     3661 ttaataataa cattgataat attttctatt ataagtactg tgtgcattat gtgtataggt
     3721 ttaatttata cacataataa tatgtttgag ttaggataca aggacattca actaaaagaa
     3781 atttctttaa gtggaataga aatatcagta agtaatgtac tttcttgtgt tgaggaagct
     3841 attgatgtat ctaataatga agacgaattt agagaatatt tcttaggaaa taatatgatt
     3901 gaatgtataa aaaaaataaa agatacatct tatagtagtt tagaagaact ttctttaaaa
     3961 gtagaaaata atactattta tgacagagga gatttttttg aatttgatat agaaagtaat
     4021 gttagagaaa aagaattttt aaaagcaata aaagtaagag tgaaaatcaa aaatccatgg
     4081 ataggaattg acattgaaga ttatatggat aaatctaata acaataaaat tgatgacaaa
     4141 acattcaata attataaagc atctgaaaat aatgaaattc taaatcaatt taaaaatgaa
     4201 agtgaaaata taaggaatca agaggttggt ggatttacag aagaagatga agaagatgaa
     4261 gaatatgaag aagaacttaa acaaaacata attgaaaata taaagaagaa ttttgatgaa
     4321 aaaaacctag tacaggtata taagtatagg ggtgtataac tttgaaaaaa gaaggaatgt
     4381 gactgtaata gaattaatag tatctatcag tataatactc ttgatagtta cattatcttt
     4441 tccaaaagat aatcttgaaa atcataaaat aaatttattt gcacgtcaac tttgttcaga
     4501 tataaggtat gttaggcgaa ttaatatgtt tagcaactac aatacatata tatattatat
     4561 aaatcaaggt aaacacgaag gttatgtatt gagaaaagat ggtaaaaaca ttaaatcaat
     4621 aatgctacct aaaaatgcaa aaatagctag gagtattgaa ataataaagt ttaaatctga
     4681 tgggtcgcct caaaaggcgt ctacaataga gatatataat aatagattaa gaaaaactat
     4741 aacaataaca cctgtaagtg gaagagtact tctgaaagag ggaaaatatg aaacataaat
     4801 atggttactt gcttttggaa agtgtcgtat cattgtcttc aatggtgata attatactag
     4861 ttttatattc tatatttttg tctacaataa acttaaagtt aaaagtagaa gataagatag
     4921 aactacaaca acaaagtctt gaaatcataa aatctatgga aggaattatt agcaattcta
     4981 tgggaataat gaatgtatct aattatgaag aaacatttaa aaagacaaca tctataaaat
     5041 gtagatattt agatgaaaat aataatgaag aaggcataag taataaagaa attatattga
     5101 atgaaagacg aaataagtta tttgtaaatt ctttaaatgg cgaaagtagt caagcaggtg
     5161 gttatgaaat tggagattat gtagatgaga tgtatgtatc aattactaat aatggtcaat
     5221 atgttaatat taaattgaaa ttatcaaaac gtagtcaaaa atatgagact gatttcaaaa
     5281 taaaagtatg gaattttagt gaaagtatat agaaatacat aaatttaaga gaaagaagtg
     5341 acatcaataa gtcatataaa atataattat tgagaactat ataactaaat aatatcaata
     5401 tgtaaagtgt atagattaag aatatagagg ataataataa aaatatagaa taagtaattt
     5461 gccaaatact tgtttatatt gtataactta tgatattatg atattagtaa aaatttgtaa
     5521 tcctgacttg acagaattca agtaagagat ataattttaa aggcatagta atgtaaaatt
     5581 tactaaaaaa taagaaattt attttaatat ggaggaacaa aaaatggtat cagcaggtga
     5641 ttttagaaaa ggtgttacat ttgaaaaaga tggacaacca tgtttagtag ttgattttca
     5701 acacgttaag ccaggtaaag gagctgcttt cgttagaact aaatacaaaa acttaaaaac
     5761 aggagctata agagaagaaa gtttcaatcc aagtgaaaaa ttccctaaag ctgttataga
     5821 tacaagacaa atgcaatatc tatacaatga tggtgagtta tattatttta tggatcaaga
     5881 aaattttgaa caaataccat taaactatga gcaagttgaa gatgcaatta agttcttaaa
     5941 agaaaatgaa gttgctacaa taagatttta ccaaggacaa ccattccaag tggaagcgcc
     6001 aaactttgca gagctagaag ttacagatac agagccaggt ataaaaggtg atacagcaag
     6061 taatgtaact aaagcagcta cagttgaaac tggagcagtt gttcaagttc cattatttat
     6121 aaatactgga gataaagtaa agatagatac tagaacaggt gaatatttat caagagtata
     6181 aaaatttatg tatataaaag cttatatgtc ttaataatat aagatatatg agcttttagt
     6241 ttatatataa aaaagaaagc gaggggtgtt acatgaaaca tttatatgaa gaagttgcat
     6301 acttaaaagg cctagcagaa ggactagaaa taagtgctga aagtaaagaa gggaaaatga
     6361 tacataaaat agttgatgca ttagaagtat ttgcagatgc tattgtaaca ttggatgaag
     6421 aacaagaaga acttcaagat tttgttgaat ctatagatga agatttagca gatttagaag
     6481 aagatattgc tgacatggaa gaagatcttt atgaagaaga tgacgatgag gatgatgagg
     6541 acttcagcta tatagaaatg gaatgcccaa attgtggaga gttagtagaa atagacgaag
     6601 atttgttata tgatgatgaa gtagatgttg tttgtcctga ttgtaaggct gtgatattat
     6661 catcagaaga tgattgtgat gatgattgta catgtggtgg ttgttcaagt tgtgatgata
     6721 gagagtaaaa attcataaga attaaaactg cctatgatgc ctatgagata ttttgcttca
     6781 taggtagttt tatttttatg aggaagggta ttatgtattt tggttttgca atacttggac
     6841 aaaaatagta cactagaatt tatagaatag gattttggag ggatgtaaat atgaaaataa
     6901 gtaaaaaatt actagataat atacaaagat ttgaaaatgt tataaattat aaatttaaaa
     6961 ataaagaata tatactagaa gctcttactc atagttcata ttctaatgaa aataaaaaat
     7021 ataattttaa cgaaagattg gaatttttag gggattcggt tttaggcata gtgataagtg
     7081 attatttatt taatgaagaa gctaatctgc ccgaagggga actaactaaa cttagagcaa
     7141 atatagtttg tgaagattca ttaagtgaag tagctaatga tataaattta ggtattcaca
     7201 tgttattagg tagaggagaa gaagcaacag gaggaagaca taggacttca atattagcag
     7261 acgcatttga agctgtaata gctgctatat atcttgatgg aggttttgaa agtgcaagac
     7321 agtttatact tcatcatatg gaaaatataa tttatgattc aagaaaagga aatatattta
     7381 gagactacaa aacgcatctt caagaggtgt tacaagggaa tggagaaaat aatatctggt
     7441 acaggcttat agaagaaaaa ggaccagacc ataataaaag atttgtaatg gaagttggaa
     7501 ttaatgacga tgtacttggt ataggagaag gcaagagtaa aaaagaagct gaacagttag
     7561 ctgctaaaat agctttaaag aaaaaattat gggaaaaata aatataatat gtaaaaatat
     7621 aaaaagaaat atttatatta gaaggatgtt gtagatgaaa aagagaatta tacctatatt
     7681 tgttcctcac aaaggatgtc ctcatgattg catattttgt aatcaaaaaa aaataacagg
     7741 tgtgagtact gatgttacta gtgaagatgc aagaaatata attgaggaat gtttagagac
     7801 tatagataaa gatgcagatg tagaaatagc attttttggt ggtagcttta ctgctataga
     7861 tattgatata caaaaaagtt tattatcagt tgctaaagaa tatgtagaaa aaggtcttgt
     7921 aaaagatatt agaatgtcta caagaccaga ttgtattgat gaagatatat taagtatgtt
     7981 aaaagaatat aagacaagta taattgaact aggagtacaa tccttagatg aaaaagtatt
     8041 attagatagt ataagaggtc atcaatctga aattgtatac aagagttcaa agatgataaa
     8101 aaatagtgga atcaaacttg gattacagat gatgattggt cttccagctg acacagaaga
     8161 aaagtgtata tttacagcaa aaaaatttgt agagctaaaa ccagattgtg ttagagtcta
     8221 tccaactttg gtagtaaaag atacaggtct tgaaaagtta atggaacaaa ataaatataa
     8281 tccatttact cttgaagaga gtgtaaatat agtaaaaaaa gttcttgtac tgttttatgt
     8341 taataatatc aatgtaataa gagttgggct tcaagctact gatgatatac aaattggaaa
     8401 agctgtttta gcaggaccat atcatcctgc atttagagag ttggtagaag cagatatgat
     8461 aaaagattat ttagaatttg ttattttgca aaataaaaat ataaagcaaa tgttggtcaa
     8521 atctaataaa aagaatattt ctaaaatcat aggaaataaa aaaacaaatg ttaaatatat
     8581 ggaagaaaaa tttggtgtgc ttttaaaaac acaagaatca gatttagata taaatcaatt
     8641 agaaattgtg ttagatggga aaagtttaat tagtgctaat atgagagaca ttcatagaaa
     8701 attgtatgac atttatgacc tttagtttta ataatgaagg gagagaaaag atttgtattt
     8761 aaaaagacta gagttaaagg gatttaaatc ctttccagta aaaacagata ttatttttaa
     8821 agaaggaata acagccatag taggaccaaa tgggagtgga aaaagtaata tatctgatgc
     8881 tgtaagatgg gtattaggag aacagagtat taagagtctt agaggtgata aactcgaaga
     8941 tgtaatattt gcaggtacag atacaaaaaa acctatgaat tattgtgaag tggctcttac
     9001 tattgataat tctgaaaatc aattggaatt agattttaca gaagtaacta taagaagaag
     9061 ggcttataga aatggtgaaa gtgaattttt tctaaataat aaaagttgta gattaaaaga
     9121 tataaaagaa gtctttttag atactggtat tggaaaagat ggttattcaa tcatagaaca
     9181 aggaaaagta gatgaaatat taagtaataa tcctttaagc agaagaaaag tttttgatga
     9241 agcttgtggc atatcaaaat atagatataa aaaacaggaa gcagaaagga atttaagtaa
     9301 tacaaaggaa aatttagaaa gaatagatga tgtatatata gaaattgaaa atcaattaaa
     9361 gcctcttttt aatcaacaga caaaggcaaa aaagtacttg gaaataagtg aaaaattaaa
     9421 gacacttgaa gtaaatagct ttataagaga aattgaagga atagaaaaag aattaagtga
     9481 ggttaatgaa catcgtaagg ttattgaaaa ggagctcaat gaaaaagaag aacaaaaaaa
     9541 tgttgtagag aaaaagcaag aagatattaa taaagaggta gaggtattac aagatgtaat
     9601 tgaaaaatct gtagattata taaattcaat taaaggagtt atatcaaaaa aagagtccca
     9661 aataaattta attaaggaaa gaataagaaa ttttacgaat gaaattagta gaaaaaattt
     9721 agaaataaag gatataaaag agaagctaaa tgaaaataag caatacataa aagaattaga
     9781 aagcaataaa ttatcaggta gtgaagaact atctaccttg caggaaaata taaaagtatt
     9841 ggaaggaagt aaagataaac aaaaaattaa gttagaatcc ctaaataatg agatagaatt
     9901 attgaaagaa agtataattg atatattaaa taagaaacaa gagtttagta ataagctgtc
     9961 tactttaaac gcaaataaag aaaatatgaa tatcagagat gaaaatataa attcagagat
    10021 aacagagtta aacaaaaata ttgaaataaa atcatctgaa ttagatacta taaataaaga
    10081 atttaatatg caaaatgaaa atttaaagaa tgtaaataat agacataaag aattatcaat
    10141 taatttgcag gattcaatta gtgaacacaa taaactagaa gatgaaatac agaaaagtaa
    10201 atataattta aatggatata attcaaagtt aaatgtctat atagatatgg aaaatcatta
    10261 tgaaggattt aatagaggag taaaagaagt actaaaaaat aaaaatttaa aaggagttca
    10321 tggagcatta ggacagatta taaatgtacc tgaaaagtat gagaaatcta tagaagcagc
    10381 tcttggtgct tatatgcaaa atataataac tgataatgag tttagtgcta agtttgcaat
    10441 aaactatcta aaacagaata atttaggtag agtaacattt cttccattaa atataattaa
    10501 atcaaataaa ataagtctag gaaatttaaa agcaaatact aagttcatag gaatagcaag
    10561 tgatttaatt acatttgatg aaaagtatag aaatataatt gaaaatatac ttggaagaac
    10621 aatacttata aataatatag atgagggcat aaaatttgca aaagaaactg gtcataggtt
    10681 taaaatagta actttagatg gtgaaatctt aaatccagga ggttctttga ctgggggaag
    10741 tctaaaaact aatgggaata tactatcgag aaaaagatac attaatgaat atacagaaaa
    10801 gataagtaat atcaagaatg aaatttcaca tctagaatta aaaagagaat ctttagacaa
    10861 agatgtgaaa aatatcaaaa atgaaattga ttcacatgaa agtaaaataa aagatttaga
    10921 aaaaagtata ataataaaaa gtacaagtat aaagaatgtt gagtctgaaa ttgaatcgtt
    10981 aaaaggtagt attactaaat tagaaaatga gaagaatgat ttaaattcta acctaaatta
    11041 tacacttgaa aaaagtgatg atgttagaaa agatatggaa gagttagatg atttatacaa
    11101 taaaaataaa gaaaaaattg atgcattgaa tgaggaaata aaaagataca atgatttata
    11161 tgataaagaa aaatctgaat ttgatgaatt gaacttaagt ttagtgaaga aaacagaagt
    11221 ttacaatagt atagttagag atataaaaag aatatctggt gaaaattgtg agctagaaga
    11281 aaaaaataaa caattagaag agtcattaaa ttatgaagaa catgaaataa ttaaattaca
    11341 agattctata ctcactgaag aaaaagaaaa agaaaattta acaaaacaac taggggatag
    11401 taatagaaat cttgaaacta gaaaaatagc aaaagatgat ttaaaaaata gttttgatga
    11461 aattaataaa gaattaaaaa ctatagatag acagcatata gaacttaaag aaagtttatt
    11521 taaagttggt ggaagattag aaagattaaa aactagtcaa gacacatata taaataagtt
    11581 gtttgagcaa tatgatatga ccttagtaca agcattggaa attaaagatg aagatttgga
    11641 tatagataga aaattcttag aaagtctaaa gagagaaata agaagtcttg gaaatataaa
    11701 tatagactct ataaaggaat atgaggaaat aaaagaaaga tacgattttt atagcgaaca
    11761 aaagcaagat ttagaagagt ctatggaaga gatagaaaaa cttatacata ctttagaaga
    11821 aaatatgaaa tctgaatttg aaattaagtt tgaagaaata agtaaaaatt ttaaatatgt
    11881 atataagaga ttatttggtg gaggttgtgg agaattaact attttagata aagaaaattt
    11941 attagaaagt gatatactaa taacagctca acccccaggt aaaaaaatga aaaatttaaa
    12001 cctattatct ggtggagaga aagctttaac ggcaataagt atattatttg ctatattgat
    12061 tacaaagcca acaccatttt gtatattaga tgagattgaa gcgccacttg atgacgcaaa
    12121 tatatttaga tttggagaat ttttgaagga tttatctaaa gagactcaat ttatatcagt
    12181 aactcatagg agaggaacaa tggaagctgc tgattatatc tacggagtta caatgcaaga
    12241 aaaagccatt tcaaaagtta ttagtttaaa attaaaagaa gctcaggaaa taacagatat
    12301 tatataactt agattgacat aggaggaata acagtgttaa aaaaattatt tggttttgga
    12361 aaagacaagg aaaaggaaat agaaaaaaaa gatgctgaag aagaaataga agttgaagac
    12421 agtgttgata atttagaaaa cttggaagaa actatttttt caggacttga agaagaagtg
    12481 atagataaag tggaagatat tgaagaaaaa aatgaggaaa gtgtcaataa tgaaggcact
    12541 gaagaaatcg aaaactttga aaaaatagaa gttgagatgg atagtaatgg aaaagaagta
    12601 gaaaatattt caaataatga ttctcaagaa gagataatcg aagaattaga aaattatgat
    12661 gaaattgagt ctaaagagat agaagataaa gaagataaga aagttaactt gtttgaaaga
    12721 ctaaaacaag gattgacaaa agcaaagcaa ggaataactg atagaataga tgaagtttta
    12781 aaatcatata caaaaataga tgaagaatta cttgaagatt tagaggaaat tttaataaca
    12841 gcagatgttg gtgtaaatac aactatggat ataattgaaa gactaagaga taaaataaag
    12901 caaaaaggta ttactgaacc aataaaggta agagaagaac ttaaatctat agttgaagat
    12961 attcttacaa atgaaaactc tacactagat atagaaccag caccatgtat aatattaatg
    13021 gtgggtgtaa atggggtcgg taagactact acaataggta aactagcaaa tagatacaaa
    13081 aaagatggta aaaaagtttt attagcagca gcagatacat ttagagcagc agcgacagag
    13141 caattagaaa tttgggcaaa tagaaccaat gtagacataa taaaacatca agagggagca
    13201 gaccctggag ctgtagtatt tgatgctata aaagctgcaa aagctagaaa aacagatgta
    13261 ttaatatgtg atacagcagg aagattacac aataaggcta atcttatgaa tgaacttgga
    13321 aaagtattta aaatagtaga tagagaattt ccagaagcaa aacgtgaagt acttcttgtg
    13381 gtggatgcta caacaggtca aaatgcagtt gttcaagcaa agacatttaa agaagttgca
    13441 gatataacag gtatagtact tacaaaactt gatggaactg caaaaggtgg agtagtcctt
    13501 gcagtaaaat cagaagtaga tgtacctgta aaacttattg gagttggaga aagtgtagaa
    13561 gacctacaag atttcaatgc aaaatcattt tcagatgcat tatttggtaa ctaatgttct
    13621 tttaaaaata atggttgaca agagtgttgt tatgatataa aatacaatag tgtaaagtaa
    13681 ataaccttta cagtatactg ctaggaagtg taagatatga atatagaaaa aatggtcgaa
    13741 attggattgt tatttgaaca gtataaagaa ttattaactg acaagcagaa agaaatagtt
    13801 gctctatact atgaagaaga ttattcttta ggtgaaatta gtgaaaatct gaatgtatca
    13861 agacaaggtg tttatgatac cttaaagcgt tcagaaaaaa tactaagaga ttatgaagaa
    13921 aagttacact tagtatcaaa gattcaagag caggaaaaaa atattaagat tataaaagat
    13981 aaaattattg acattaaaga agatttattg cacaatagag attgtgctaa tttaatccct
    14041 aagctagaaa atatagaaga tgtatgtagg gagatgataa aatgatattt gaaggattgt
    14101 ctgataaact acaaggggca tttagtaagt taaaatctaa aggaaaactt acagaagtag
    14161 atgtaaaaaa tgctatgaga gaagttaaac ttgctcttct tgaggcagat gttaacttta
    14221 aagtagttaa agatttcata aaaaaagttc aagagagatg tgttgggcaa gaagttatgc
    14281 aaagcttaac tccagctcag catgtaatca aaattgtaaa tgaagagctt acaagtttaa
    14341 tgggagatgt acaaagtaaa gtaatgattt cctcaaagcc tcctacaatt ttaatgatgg
    14401 ttggattgca aggtgcaggt aagactacta cagcaggaaa acttggagga tattttaaaa
    14461 agcaaggtaa aaaacctcta ttagtagctt gtgatattta tagaccagca gctataaagc
    14521 aacttcaagt tgttggagaa aaattggata tacctgtatt caatatggga gataaagaaa
    14581 atccaataaa tattgcgaaa gcaggtttaa gccatgcgat taaaaatgct aatgatttag
    14641 ttattataga tacagctggt agacttcata tagatgaagt acttatggat gagttaaagt
    14701 ctattaaatc agaagttaag ccacatgaga tacttttggt tgtagattct atgacaggtc
    14761 aagatgctgt aaatgttgca gaaagcttca atgaggcact tggtgttgat ggagtagtat
    14821 taactaaatt ggatggagat actagaggtg gtgctgcgct ttcaataaga gcagttacac
    14881 aaaagcctat aaaatttatg gggatgggag aaaagctaga tgatatagaa cctttccacc
    14941 cagatagaat ggcatcaaga atattaggta tgggagatgt cttaagttta atagaaaaag
    15001 cacaagaaaa tatagactta gagaaagcta aagaattaga gcaaaaaatt aaaaaacaag
    15061 agcttgattt tgaagatttc ttacaacaaa tggaacaaat acaaaatatg ggacctcttg
    15121 acaagatact tggtatgata cctggtatgg gaaatgtaaa agaccaactt ggagatgtag
    15181 atttgaatgg caaagaaatg aagagaacaa aagccataat tcagtcaatg actacatatg
    15241 agagaagaaa cccaggagta ttaaatgctt caagaaagaa aagaatagct aaaggcagtg
    15301 gaactagtgt acaagatgta aatagactta taaaacaact taatgaaatg aaaaaaatga
    15361 tgaagatgtt tgctggttca caaaagagta tgaagaaaag gggcggctta ggcggtttac
    15421 ctttttttaa ataaaatata aaaaagtaaa aattattcgg aggtgactgt aatggcagtt
    15481 aaaataagat taaaaagaat gggagctaac aaaaaacctt tctacagaat agtagtagct
    15541 gattcaagag ctccaagaga tggaaaattt atagaagaaa taggatacta taatccaatt
    15601 tctgaaccta aacaagtaag aataaatgat gaaaaagcta taaaatggtt agctactggt
    15661 gctcagccaa cagaagtagt taaaaaatta cttgtaaaaa acggagtaat agaaaaattc
    15721 gaagcttcta aacaagcaaa ataatttttt cttggcaatt agtttaagta agggctcatt
    15781 gctaaggtta ctttaggaga tgaaatatat gaaagagcta gttgtagata tagctaaggc
    15841 tctagttgat aatcctgact cagttgttgt tgaggaattt gaagataatg atggtatcgt
    15901 cttaaagctt acagttgctc aagaggatat gggtaaggtt attgggaagc agggaagaat
    15961 agctaaagct ataagaactg tcgtaagatc tgttgcaaat agagaaaata taaaagtttc
    16021 tcttgagata gtataaaata ggaggattag gtataatgcc tagtcctttt tatataaatg
    16081 cgtagtttaa gattttatga tgatatgacc acataagatt aaatttttta agaaggtgat
    16141 aacatgaaag aaaaattaac tcattttaag gtaggtcaaa tagtaaatac acaaggttta
    16201 aaaggtgaag taagagttta tccgcttact gatgatatag agaggtttga tgaattaaaa
    16261 gatttctatc tgggaaaaga tttaaataat aaattggctg ttgaaaaagt aagatacaaa
    16321 ggttctatgg ttataatgaa gataaaaggc atagacagca tagagaaagc agaaaaacta
    16381 aaaaataaat ttatgtatgt agctagagaa gatagcagag agcttgaaga aggcgaattt
    16441 tttatagcag atatgattgg aatggaagtt ttaactgtag atggaaaata tgtagggata
    16501 ctagaagatg ttcttcaata ctcagcaaat gatgtctatg tagtaaaagg tgaggaagat
    16561 aaagaattta tgataccagc tataaaaaaa tttgtaccta caatagatat tgatgaaaga
    16621 aaaatgatta tagaccctat taagggaatg atagattagg tggtaatatg agatttcata
    16681 taatgacact ttttccagaa atatttaatt cgtacatgga tgaaagtata atgaagagag
    16741 cagttgaaaa aggtataatt gaggttcata tatataatat aagggatttt tctaataata
    16801 aacataaaaa agtagatgat tatccatttg gaggaggagc ggggatggta atgactcctc
    16861 aacctatata tgacacatat aagcatataa taacaactca taatataaat aacccaagtg
    16921 taatttatct tactccaaaa ggtaaggtat acaatcaatc tatggcaaag cagatgtctt
    16981 taaaagaaga tataatacta ctatgtggtc attatgaagg aatagatgag agaatcattg
    17041 atttaatagt tactgatgaa atttcaattg gagattatgt attaactggg ggagagctcc
    17101 cagcacttat aatgatagat tctatatcga gattaatacc aggtgtatta aaccaagaag
    17161 aatcatttga agaagaatca tttaaagaca acttattgga atatcctcat tacactagac
    17221 ctagagattt tgaaggatta aaagtaccag aagtgctttt atcagggaat cataagaaga
    17281 tagatgagtg gagaagagaa gaatctataa ggataaccaa agaaagacga tttgatttat
    17341 ataaaaaatc taatgaaaaa taaaaatata atgaaaaatt aatatatatg tatacaataa
    17401 agtacctata aatggaatta aaaacataaa agtgcttgta ataactattg aagtatagta
    17461 taatataaaa tgttgaaaat acgggcgttc ctctgtttac actggtcggg tagttaagaa
    17521 cgtctatgat atctaggagg aagaaagatg aatgaaatgc taagagcaat tgagcaagaa
    17581 caattaaaaa atgaagttcc taattttgga cctggggaca cagttaaagt acacgttaga
    17641 attatagaag gaaaaagaga aagaatacaa gtattcgaag gtgtagtatt aaagagacaa
    17701 ggtggaggag caagagaaac tttcacagtt agaaaaatgt ctttcaatgt tggtgtagaa
    17761 agaactttcc cagttcactc tccaaaaata gaaaaaatag aagttactag aaaaggtaaa
    17821 gtaagaagag ctaaactaaa ctacttaaga ggtagagtag gtaaagctgc taagataaaa
    17881 gaagctagaa ataaataatt aaaaacttta cttataaatg gggggctgat ttatcagtcc
    17941 tctttctatt ttaatgaaaa ttacagcaaa taattatact agaaataaat agaaatattt
    18001 gttgaaaata tacattattt atctatgaat ttatagcttt taaaatgaaa aataaaattg
    18061 gtaaatattg cattatattt tgtaacaata ttaaaaataa gagaacagta ttatgtaaag
    18121 tatgacgtta aaaagatgac aatattatag tttgttaaga tagatttttt aagtggaatt
    18181 aggatataat aatataatat taataaggag tgatacatgt gtcaaatgaa tacgaagagt
    18241 atcttatgaa tgacaatcta catataaact ggtatccagg tcatatgaaa aaaactaagg
    18301 agttagtaaa aaataactta aaattgattg atgtagtaat tgaacttttg gatgctagaa
    18361 tcccgtttag tagtaaaaat ccagacatag acaggcttgt aggagataag ccaagagttg
    18421 tcgtacttaa taagagtgat atggcagata gggataaatt gaatcaatgg attgaatact
    18481 ataagaaaat taatatcaaa gctataccag tagatacaat aaaaggcgtt ggtataaata
    18541 agataatcga agaatgtaaa aatgtaacta gagaaaaaat gagttcttta aaagacaaag
    18601 ggagaaaaga aagagctata cgaataatga tagttggagt tccaaacgtt ggtaaatcgt
    18661 ctcttataaa caaattaaca ggtagaaaaa gcactcaaac tggagataag ccaggagtaa
    18721 cgaaagggaa acaatgggtt agattaaagg gaaatcttga acttttagat acacctggaa
    18781 tactttggcc taagtttgaa gaccaagaag ttgcattaaa tttagcattt agtagagcta
    18841 taaaggatga gattttagat actgaaactt tggctttaag acttatagag aaacttatga
    18901 aaatagaacc tgagaagttg aaagccagat ataagttaga ttgtctagga gaaactccaa
    18961 ttgaaactat ggatatgatt ggccataaaa gagggtttat aacaggtaaa aaagaactag
    19021 attatacacg catagccaca actgtattaa atgaatttag agatggaaaa ataggaaata
    19081 taacattgga agttccagaa aacgttaaaa gatagatttt aaaacaaaat caaaaattta
    19141 atgttaaaaa tttaaaaaag ccttttagca aaggcttttt tatttttttg aaatatagac
    19201 aaatatctcc atataaaagt ataaattagg ttagaaaacc tttaaaaata ttaatttaag
    19261 acaaatatac ctaatatttt gaaaattaaa atgagtgtga ttatttaaaa ttttagcata
    19321 ttgacttgct tgtttcgtat aatatataat aagcacaaat aaatacaata tattaaaaat
    19381 ttaatttatt taattttatt aatttaataa taagttctta ttgtttaatt caacttactt
    19441 atgcaagcgt aaacaatcga gacattttat taatttaata aggttaaaaa gggggatttt
    19501 ataatgggta agaccaagga tgttatagta tttggatttg cgttatttgc aatgttcttt
    19561 ggagcaggta acttaatatt tccaccttac ttgggaataa ttacaggtcc agaatggtta
    19621 attgccttct taggatttac gtttgctgat gctggattgg cacttctagc agtaatggct
    19681 acagctaaat tcgatggaaa cgtagttgaa atgtttaaaa gatgtggtat gaagttagga
    19741 attcttatag gttgtgcaga tattttatgt ataggaccat tcttagctat accaagaaca
    19801 ggtgcaacaa cttatgaaat gggtataatg cctttgtttg gaacttcaat tccagtgtta
    19861 ttattctgta tacttttttt tgcgatttca tatgtactaa caataagacc ttctaaggtc
    19921 gtagatatag ttggacagtt tttaactcca gctcttctaa tagctttagc atttataatt
    19981 ataaaaggta ttatatcacc tcttggagat atagtagata aacctatgat acctaatgta
    20041 tttgcagaag gtataggtca aggatatcag acaatggatg cttttgcagc aatagcactt
    20101 gcatcagtgc taatagtatc tttaaatgat aaaggatatt ctactattag tgacaagttg
    20161 aaaatgattg gtaaggcggg tgttcttgct tgtggaggat tagctctagt ttatggagga
    20221 ttatgtttcc ttggggcaac tgtatcaaca atgtatggaa cagatgcagt acaatcacaa
    20281 gtaatagtaa atataactga aggattgctt ggaaatgttg ggaaagcgat tttagcagta
    20341 gtagtatcat tagcatgttt aactacatct ataggtctta catctgccac aggtcaatat
    20401 ttctctagac ttacaaaagg taaattgagt tatgaaaaaa tagtattagc tgtatctgta
    20461 ttcagtgcag tagtagcatc ttttggtgtt ggaactataa taaagatagc atctcctata
    20521 ctaagtatag tttatccacc atcaattgtt ttaataattt tagctttctt caatgaaaaa
    20581 attaagaatg ataatgtata caaaggagca gtatatatgt cattgtttgt aagtatacta
    20641 acagttatat ctagttatgg agtagctgtt ccagtagtaa attctttacc tcttaattca
    20701 ttaggattta actgggtggt gccagttata atagctggaa taattggtaa ttttatacca
    20761 tcaaagagtc aatctaatac acttggaaca aactaagttt tgtttaatta aaatataatc
    20821 aattgtaaat aaatgagtta actaaaagta aaaggagtta tttgtacaaa taactccttt
    20881 tatttttaga ttct
//
LOCUS       DAMJVK010000068        20778 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.069, whole genome shotgun sequence.
ACCESSION   DAMJVK010000068 DAMJVK010000000
VERSION     DAMJVK010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20778
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.069"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            60..1016
                     /locus_tag="PC414_002568"
     CDS             60..1016
                     /locus_tag="PC414_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HDF2464956.1"
                     /translation="MYILNLNTRETIEDFRDKFYVAENSYLILSAPKNLKLLKETLDI
                     DEITFNDCLKFDEITKLDLFDNYDFLSLNTFELRDGEAVIEEVNMYLSDNFILVVVNE
                     EHFLFEFVKNIILKNSQLEKNPVINLFKINYLILREVIKNGFESLEKVEELILQIEDE
                     MMDNINKNHVSRISDVRGLTRIIVKNTRPLLYIGDRIVKENIRYLKYSNVKKYNLENF
                     QGIDFGIDKLYNFALSTRELADKLLDIYSSRVGEKTNNLITKLTLLTAISAPLTIITG
                     IYGMNFRYMPELNWIYGYPATLFFMLCIIFVGIIIFKIKKLL"
     gene            1193..1348
                     /locus_tag="PC414_002569"
     CDS             1193..1348
                     /locus_tag="PC414_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2464957.1"
                     /translation="MKGILKELTAKLYSFFYFMFWGFYFSAGYFFYKNICTSFLVRPY
                     FSDFEHK"
     gene            1478..2491
                     /gene="aroF"
                     /locus_tag="PC414_002570"
     CDS             1478..2491
                     /gene="aroF"
                     /locus_tag="PC414_002570"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HDF2464958.1"
                     /translation="MIVVLKMGADKNEVKKLIEAIGREGVEVNPIDGTELTVLGLVGD
                     TSKIDAKRIEANKVVEKVMHVVEPFKKANRKFHPEPSIINVNGMEIGSKKIAMIAGPC
                     SVETEDQIVSIAKDVKKSGAGFLRGGAFKPRTSPYAFQGLKYDGLDLLKKAKEKTGLP
                     IVTEIMSTQDIDIFEENVDVIQVGARNMQNFDLLKELGKTNKTILLKRGLSATIEEWL
                     MSAEYIMAGGNENVVLCERGIRTFETYTRNTLDLSAILAVKKLSHLPVIVDPSHAAGK
                     SWMVDSLSKAAIAVGADGLIIEVHNDPAHALCDGKQSIKPNEYDELISELKTIASAVG
                     REI"
     gene            2513..3577
                     /locus_tag="PC414_002571"
     CDS             2513..3577
                     /locus_tag="PC414_002571"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HDF2464959.1"
                     /translation="MEEKLLVNLKENSYNIFIKKNILKNIGEEIKKIYFGNKIFIVTD
                     ENIAKLYLDKVKNNLSESGFEIKSLILKSGEETKSFNTLPKIYDSLLDFKLTRKDLII
                     TLGGGVIGDLGGFAAATFLRGISFIQIPTSLLAQVDSSVGGKVGVDLERGKNLVGSFY
                     QPKAVFIDPNVLLSLPEKFYRDGMAEVIKYGCIKDKTFFNKLLSLKNREEVMQHIEYI
                     IHKCCFIKKTVVEADEKDFGDRMLLNFGHTLGHAIEKYYNFTGYTHGEAVAIGMYKIT
                     LESEKIGITKKGTSELIKSILMNYNLPYEIELRDNKSIVDAISLDKKNLGDTLKIILL
                     KDIGESIIYNTTSKFFQEVR"
     gene            3577..4890
                     /gene="aroA"
                     /locus_tag="PC414_002572"
     CDS             3577..4890
                     /gene="aroA"
                     /locus_tag="PC414_002572"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDF2464960.1"
                     /translation="MGSLKIYPSKLSGDVKIPPSKSMAHRAVICSSLSNGKSRISNID
                     FSDDIIATIRAMTSLGAIIEKKEDILEISGIFSKEGILNRENQLNQPKLTIDCNESGS
                     TLRFLVPISLTFDGVKRFIGRGNLGKRPLDTYYEIFDRQNIKYSYKENQLDLIISGKL
                     KPDEFRVKGNISSQFITGLLFILPTLESDSKIIITTELESKGYLDLTLSTIKDFGVEI
                     INNNYKEFIIKGNQTYKARDYKVEGDYSQGAFYLSADAIGEDISILDLKEDSLQGDSE
                     VVEILSRMGMEILREGNKIKGITNGLNGTLIDASQCPDIIPVLSVVASLSIGRTTIIN
                     AGRLRIKECDRLHAINVELSKLGANIEEKEDSLIIEGVSKLNGGVEVWSHKDHRIAMT
                     LAIASCRCDKPIILKDFECVSKSYPHFFKDFKMLGGRIDEWNMGK"
     gene            4868..5947
                     /gene="aroC"
                     /locus_tag="PC414_002573"
     CDS             4868..5947
                     /gene="aroC"
                     /locus_tag="PC414_002573"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDF2464961.1"
                     /translation="MSGIWGNNLKVSIFGESHGNAIGINIDGLPSGIELDLDNIDKEM
                     KRRAPGKNSISTSRNESDIPEILSGYFNGRTTGTPLCAIIRNSDTRSKDYGELKNLMR
                     PGHADFTGNVRYSGFNDYRGGGHFSGRITAPLVFCGAICKQILSQKGIEIGAHIKKIK
                     NIEDMSFDYVNISKQQLSNLQTLELPLLDLSKEEAMKNTIIDAKNQGDSVGGIIECAV
                     VGINVGLGNPFFDSVESTLSHLLFSVPAVKGVEFGLGFELADMYGSQSNDEMYYEGNQ
                     VKSKTNNNGGIIGGITTGMPIIFKVAIKPTPSISRQQNTVNIKDKKDDILYIKGRHDP
                     CIVQRAIPVIEAVTAIGIFDLMKGR"
     gene            5950..7146
                     /gene="pheA"
                     /locus_tag="PC414_002574"
     CDS             5950..7146
                     /gene="pheA"
                     /locus_tag="PC414_002574"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HDF2464962.1"
                     /translation="MEDLTRYRNEIDNIDKELIQLFEKRMNTVLEIARHKMKNNTTIL
                     QKDREEKVLSRAVDNLDNKAYSQETVQFFNSIMEISRGLQKRLIDNDGEQKIEFSLDS
                     SNKKFDLSNVNRYKSLKDELNKKNILVGFPGKSGSFTEEALNKFFNKKISKKQFKEFE
                     DVFIALKNKEINYGIIPIENSSTGAISETYDLLRKYGFYIVGEECIKIDQNLIGIKDT
                     KLEDIKEIYSHPQGIGQCSKFLKQNSTWKLIPFNNTATSAELVRKLQDKTKAAIASKK
                     AASIYGLEIISPCINDIANNYTRFVVISNQIHIEEESNKMSVVFSVEHEAGKLYKVLG
                     YFAKNNINMTKIESRPMKNASWRYFFYIDFECSIYNAKVYDLLELIEHNTAYFKFMGV
                     YKNKTE"
     gene            7193..8002
                     /gene="aroE"
                     /locus_tag="PC414_002575"
     CDS             7193..8002
                     /gene="aroE"
                     /locus_tag="PC414_002575"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDF2464963.1"
                     /translation="MNFFGLVGEKLSHSVSPQIHKRVFEILNIESAYKNFEISKEDIS
                     KLDGAIKLLGIQGVNVTVPYKERIMKYLDFISPEAKRIGAVNTILLRENMLYGYNTDY
                     FGLDSMFKMANIDVQGKVAVILGTGGASKAALTYFIDSGIEKLYVSTRKKDDKKLLNS
                     KAILIDYEELKHIKGDIILNATPVGMYPNVGISPVSKSIIQNFDILIDLIYNPGETEF
                     LRIGNSMGKKTCDGLYMLVGQAIKSQEIWQDTKIDNSILDVIYNELKLEFL"
     gene            8014..8547
                     /locus_tag="PC414_002576"
     CDS             8014..8547
                     /locus_tag="PC414_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HDF2464964.1"
                     /translation="MINKTKEKLILIGMPGSGKTTIGKLLAKEYNCSFCDMDDYIIQI
                     SQKSIAELFSEGEDIFRNYETQACRELSISDKTVISTGGGVIKKDVNMEILKETGIII
                     FIDRPIQKILEDININSRPLLKNGKDRLYNLYNERINLYKKFSDIEILNDKSLNNAVY
                     NITNAVSENFKFDFKEK"
     gene            8568..9407
                     /locus_tag="PC414_002577"
     CDS             8568..9407
                     /locus_tag="PC414_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HDF2464965.1"
                     /translation="MNIVIVGLGVIGGSFAKALKKAGYENVFGVDVDLETLKKAEKAK
                     IIKKGCTTGKELFKKADLIILSIYPRLVVDFLNNNKNFFKKGTIITDTTGIKETLIND
                     VLQIIPDDIDFIFGHPMAGREKKGIDFASEQVFNGANYIITPTGRNNIKNLELVENLI
                     LEIGFKRVKKLTSQKHDEIIAFTSQLPHVMAVALINSDEEGRDTGKFIGDSYRDLTRI
                     ANMNEDLWSELFLGNRDNLLKVIENFESEVNLVKEAIFNNDKNKLIEYFKKSSIRREA
                     LEK"
     gene            9675..11156
                     /locus_tag="PC414_002578"
     CDS             9675..11156
                     /locus_tag="PC414_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HDF2464966.1"
                     /translation="MKKNRLIAKNISIAFIVLFFFSVFTFFYVGNINRVLEYETNDII
                     TVTIAGWIILSFLFLGIIIYILYSKANSQKTIEKVAYTDFVTGYSNWRKFELDVTNLL
                     KKTSQNNKYAMVIFDIDKFKAINDIYGHKKGNLILKDIADTLNELTDINETFARVSAD
                     NFNILLTYNKKEDIINIIKKIMANNELVNLSFGIYEIKDKDLSVSVYSDRASLAKSSI
                     KNNSDVNFAFFNDKLREKLLFEDKIEKEMEYALESGQFVMYLQPKYNIKLDKFCGSEA
                     LVRWQYTEKEVIYPGDFIPIFEKNGFIRKIDMYILEQACKEIRSLFDKGISPLPISVN
                     FSRVDFFKKDFIENIVNICDRYKIPYSLIEIEITESSMFGDTDTLFNVSRNLQDIGFI
                     VAMDDFGSGYSSVNMLKNIPLNVIKLDRGFFVDDKDVDKSQIVIKSIVSLIKQLGIRV
                     VAEGIETRSQIEMLKKANCDIVQGYYFSKPLPIKEFEKLVYKI"
     gene            11189..14623
                     /locus_tag="PC414_002579"
     CDS             11189..14623
                     /locus_tag="PC414_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HDF2464967.1"
                     /translation="MIRAKSLKNVLSIVLIFLFTIISFSDFAFKTNVQEEENSKINLR
                     EISLQSSNAIKIKLDSSLSFIKSVANMVSYFNTIDNSKIVPLLANISKESDFQRISIV
                     SPDGISHTSDGKIINVSKYQYFKKAIKGESNISEVVKSPIDGKDTFVIATPVFNKNNK
                     IIGVLYGSYYNDKLAEFIDITSFNSEGYIDIFESSGQFVLKSSHTNKLIKSSSNIYDI
                     FKKASFDDGYSYKKIYEDTKNNKSGFTSYEYKGENRYATYTPIGVNDWYVISVIPQYT
                     VRRNIDKINQLAFSLIFKVFLLFIMLIIYIIYKNNKSQKDILKANKEISSITNSIPGG
                     VQKCTLGETCNINFLSDGFIELFGYTREEIKTLFNNDFYSLIHKDDVERIKKEIFKQL
                     YKNKTVELEYRIITKNGNTVWVLDKGELITEPDGKEYFCCVTVDITRTKQLEQEMEQK
                     NLELEMISENVLGGIIITEFNDDFTIKYANSGYLSMIGYTREQLENELKYKACNLVFY
                     EDKKKALESIQHQLNTKGIAVYQHRIRRRNGNIIWVSIKGRLIVDDGIEKGIWILNDI
                     TDLKNAEQKLKINEERFRIALKQTSNTIFEYDIKTKSLLNTSKELDKYNIYKITKNVP
                     YSLVESGVIHKDFSDDFINMYENVINGAKKASCVVKMKLPDENYIWNKVTLTTIFDED
                     GNPVRAIGIQEDISKQKEAEIRYNQEGKYHSAILSEAMITYEINVSKDTFIKGNESWQ
                     KPFGIKPDNNYSQMVKTIANSIVHKDNKETFLNTFLCENLLDAYKNNVTEIKLEYRRM
                     NQENKMIWVICTMHLIKDSITNDIKALAYVKDIDKQKNEEIRLKLKAERDTLTGLYNK
                     GTTEKLINDFLNSEEASLGMHAFLIVDIDNFKSVNDNLGHVFGDKVLFEVSKNLKPIF
                     RKDDIVGRIGGDEFIVFLKNIQSFENIKKKAEKICDIFRNSYTENKKTYKISGTVGIS
                     FVPEHGTVFEELYKKADIALYYAKSKGKDKYALYNDCIYQNELENNISDNNIKLADKC
                     EKSKPVLKEILDSIDNYIYIVNPNTYELLFVNNKVSRLDLEIKLGETCYKSLLKSDRP
                     CNNCPLNGLEENKHSKFKIETNTFGKNISIASWINCIDGEYNCLIECIDSSLYKNK"
     gene            14641..15387
                     /locus_tag="PC414_002580"
     CDS             14641..15387
                     /locus_tag="PC414_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDF2464968.1"
                     /translation="MLNSITLILGIIFTIYYFYLKIVFGGISFSEIFLVVGIVLIIYQ
                     IFKKKIKEKKVFYRVLKIIISIFLIVFVVTESLIIFYPKNSLESKSDYLLILGASVKK
                     TTPSTTLKGRLDTALKYLKINDNCYVVVSGGKGSGEKITEAKAMKDYLIKNGIDKNRI
                     IEEDKSTNTYENFKYSKLKIEEHSQGKLSDLKVKIVTTDFHVLRSKILAYRNGYKNTS
                     FYASKSKLSFVPTYYTREFFALWKTIIFDR"
     gene            complement(15336..15644)
                     /locus_tag="PC414_002581"
     CDS             complement(15336..15644)
                     /locus_tag="PC414_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HDF2464969.1"
                     /translation="MNFFKENSTFYFATVEDNKPRVRPFGFIMDYEGKLYFGIVKYKT
                     SYKQLLVNPNVEISTTSKVGKWIRIKGIEKTRYEFISSLFIYYLSKMIVFHNAKNSLV
                     "
     gene            16448..20590
                     /locus_tag="PC414_002582"
     CDS             16448..20590
                     /locus_tag="PC414_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HDF2464970.1"
                     /translation="MATVSGQIVFDRNRSATIDSGDSGIANIPVVLQDIATGVRLVVL
                     TDASGNYVFTNVPNGNYRIVEAFGTTGGVPTPGNFATAIVGPVPIAEMPPISSVTSPP
                     IGSTNLDGTTPNTLLITVAGSDITNQNILNGPVIYTPIENILDSCVSVSNINLITDAD
                     NGTFGFFPPGTPSNTGPEIEPYPNVTPDFTYVVPDPTKFTPLDGEYTVQNIMTNAMSN
                     VIGAWWRIADHTTGNETGRMMVVNGFNPGAVFFKSTVQVTPNTNYLFSTWILNLFKVT
                     GYPPPQLGVRIFDQNNNILYQATLGAQIPVNTNAPEWKEVGTVINSQNNNQLTVEFFS
                     EGESAIGNDYAIDDVSLREIAVPTFTPIKTSNTETANVGDIITYTVTLDNTCDSPLTN
                     VFFRDLIPNGLEFIPGTVTVDGVSVPGVDPNTGFPLPDIGGGTGVEVTFDVVVESIPN
                     PNPTNNIATIDYSYTPVEGGIPNDFSVDSNPVPVEVISADIEVTKLSEPTIVNPGEEL
                     IYTIKVVNNGPFPSENVVLTDDVPASIVNPEYSLDGGVTFQPWTGSLNIGTLEVGETR
                     VIIIRGIVNPSTVGIITNTAVVSSTTADPNLNNNTSTIETEVNLLADILVMKTAEPNS
                     AVPGTLLRYTIQVENLGPANAENVILNDDIPASIINPEYSLDGGASFQPWNGSLNIGT
                     LNSGISLTVLIQGTVSLNSSEYIVNTATVSSTTPDPDLSNNISTIITPVNPQAGISII
                     KVADEDVAVPGEEFVYTIEIFNEGPSNATNVVLTDDIPDVILNPEYSLDGGATFQPWT
                     GSLNIGTVAPGQLIRIIIKGLVSSTATGDITNTAEVSADVPEPVTDSSTVTTPIVPSA
                     DIEVIKTSNMDTAVPGETFSYTITVTNLGPSAAQSIVLTDDIPDVILNPEYSLDGGVT
                     FQPWNGNLSIGTLDAGEIRSIIIRGTVSQTAVGTIINTATISSTTPDPNPDNNTSTDE
                     TDISPLADISVIKGNEPVAIPGGRFIYGIEIANAGPSFAENVTLTDNIPASILNPEYS
                     IDNGVTFQPWNGSLNIGTLDAGEIRNIIIRGTVSQTAIGTIINTATVSSTTPDPNLNN
                     NISTSEAEVSSSADISVVKRSNQTVVVPGDVLDYTIEVRNAGPSTAQNVTLTDNIPAS
                     ILSPEYSIDNGVTFQPWNGSLSIGTLDAGEIRNIIIRGIVSQTAIGTIINTATVSSTT
                     PDPNPDNNTSTSEIDISSSADISVIKLANKTEACVGEQIDFVIVVSNAGPENAQNVTL
                     IDNVSDKLKKAVFSLDRGVSFQPWTGSLNIGTLPAGTLRVIILRGIIKSTCLDRLTNI
                     AEITSTTPDSNLNNNISRVQVEIKQCCCNDCCCNNCSNCCCNDCGCKDCCCNCCKNDF
                     WC"
BASE COUNT         8463 a         2265 c         3452 g         6598 t
ORIGIN      
        1 attacagtaa ggattataaa aaatttttag aatgtaaatt gaaatttagg agtagatgta
       61 tgtatatact gaatttaaat acaagagaga ctattgaaga ttttagagat aaattttatg
      121 tagcagaaaa ttcttatctt atattaagtg ctccaaaaaa tttaaaattg cttaaggaaa
      181 ctttggatat agatgaaata acttttaatg attgtcttaa atttgatgaa ataaccaaac
      241 ttgatttatt tgataattac gattttttaa gcctaaatac atttgaacta agagatggag
      301 aagctgttat agaagaggta aatatgtatt tatcagataa ttttatattg gttgtagtga
      361 atgaagaaca ctttttattt gaatttgtaa aaaatattat tttgaagaac tctcaacttg
      421 aaaaaaatcc agttattaat ctatttaaga taaactactt gattcttaga gaagttataa
      481 aaaatggttt tgagtctttg gaaaaagttg aagagttaat tcttcaaata gaagatgaaa
      541 tgatggacaa cattaataag aatcatgtat ctagaataag tgatgtaaga ggattaacaa
      601 gaattatagt taagaatact agacctcttt tgtatattgg ggatagaata gttaaggaaa
      661 atataagata tttaaaatat tcaaatgtaa aaaaatataa tttagaaaat tttcaaggta
      721 tagattttgg aattgacaaa ttatacaatt tcgcattgtc aacacgagag ttagcagata
      781 aattattaga tatttattct tccagagtag gtgagaaaac aaataactta attacaaaat
      841 taaccttgtt aacagccata tcagcaccac ttacaattat aactggtata tatggaatga
      901 actttagata tatgccagaa ttaaactgga tttatggata tccagcaact ttatttttta
      961 tgctatgtat aatttttgtt ggaattataa tatttaaaat aaaaaaatta ttgtaaatta
     1021 cagatgatgt ttaattgatt ttataaaaat acatcaattg agaagctgat ttaagtaaat
     1081 aaaaaaatat tttaaattga aaatatttat agaaaatatt gacaattata ttttctttgt
     1141 tataattatt aaacaagaag aataataaat tataaaaatg gaggataata ctatgaaagg
     1201 aatattaaaa gaactaacag caaaacttta tagctttttt tactttatgt tctggggctt
     1261 ttattttagc gctggctatt tcttttacaa aaatatatgt acttcctttc tggtgaggcc
     1321 ttattttagt gattttgagc ataagtaaat tttataaaaa tgattatttt atatatttat
     1381 ataagattta gtgcttgata gattatttta gagaactcat tagtgagttc tcttttttat
     1441 tttaaatata aaattataat attgggaggt agtaaaaatg atagtagtat taaaaatggg
     1501 agcagataaa aatgaagtta aaaaattaat tgaggctatt gggagagaag gagtagaagt
     1561 taatcccata gatggaacag aattaactgt tttaggttta gttggagata ctagtaaaat
     1621 agatgccaaa agaatagaag caaataaggt agttgaaaaa gttatgcatg tggtagagcc
     1681 ttttaaaaag gccaatagaa aatttcatcc agaaccttca atcataaatg ttaatggaat
     1741 ggaaattggt tcaaagaaaa ttgctatgat agctgggcct tgttctgttg aaacagaaga
     1801 ccaaatagta tctatagcta aagatgtgaa aaaatcaggt gcaggatttt tgagaggtgg
     1861 agcattcaaa ccaagaacat caccatacgc tttccaagga ttgaaatatg atggattgga
     1921 tttattaaaa aaagcaaaag aaaaaacagg gcttcctata gttacagaaa taatgtcaac
     1981 acaagatata gatatatttg aagaaaatgt agatgtaatt caagttggag ctagaaacat
     2041 gcaaaacttt gatttattaa aagaattagg taaaacaaat aaaacaatac ttttaaaaag
     2101 aggattatct gctacaattg aagagtggct aatgtcagca gagtatataa tggctggagg
     2161 aaatgaaaat gtagttcttt gtgaaagagg tataagaact tttgaaacat atactagaaa
     2221 tactttggat ttaagtgcaa ttttagctgt gaagaaactt agtcatttac cagttatagt
     2281 tgacccaagt catgcagcag gtaaatcatg gatggttgac tctttatcta aggcagcaat
     2341 tgcagttgga gcagatggtc taataataga agttcataat gacccagcac atgcactttg
     2401 tgatggaaaa caatcgatta agccaaatga atatgatgaa ttgataagtg aattaaagac
     2461 tatcgcatca gcagttggta gagaaatata atctataaaa ggatggttaa acgtggaaga
     2521 aaaactatta gtaaatttaa aagaaaatag ctacaatata tttattaaaa aaaatatact
     2581 aaagaatata ggagaagaaa taaaaaaaat atattttgga aataagattt ttattgtaac
     2641 tgatgaaaat atagcaaaat tatacttgga taaagtaaaa aataatttaa gtgaatctgg
     2701 atttgaaatt aaatctttaa tattaaaatc tggtgaagaa acaaaatcat ttaatactct
     2761 tcctaaaata tacgatagtt tacttgactt taagttaact agaaaagatt tgattattac
     2821 tttaggagga ggggtaatag gtgatttagg tggatttgca gcagcaactt ttttaagagg
     2881 tataagtttt attcaaattc caacatcttt attagcacaa gttgatagtt ctgtaggtgg
     2941 aaaagttgga gttgaccttg agagaggcaa aaacttagtg ggaagctttt accaaccaaa
     3001 ggctgtgttt atcgacccaa atgtattatt atcacttcca gaaaaattct atagagatgg
     3061 aatggcagaa gttataaaat atggatgtat aaaagacaaa acttttttca ataaactttt
     3121 aagtttaaaa aatagagaag aagtaatgca acatatagaa tacattatac ataaatgttg
     3181 ctttattaaa aaaactgtag ttgaagctga tgaaaaagac tttggagata gaatgcttct
     3241 aaactttggc catacattag gtcatgctat tgagaaatac tacaatttta caggttatac
     3301 ccatggagaa gctgttgcca taggaatgta taagataact ttagaaagtg aaaagatagg
     3361 aattacaaaa aaaggtacat cagaacttat aaaatcaatt ttaatgaatt ataatttgcc
     3421 ttatgagatt gaattaagag ataataaatc tatagtagat gctatatctc ttgataaaaa
     3481 aaatctaggt gatacattaa aaataatact tttaaaagat attggagaga gtattatata
     3541 taatactact tctaaatttt ttcaggaggt aagataatgg gaagtctaaa aatatatcca
     3601 agtaaattat ctggagatgt aaaaatacca ccatcaaaaa gtatggcaca tagggcagtt
     3661 atttgttcat ctctaagcaa tggaaaaagt agaatttcta atatagattt ttctgatgat
     3721 ataatagcta ctataagagc aatgacatca ttaggagcta ttatagaaaa gaaagaagat
     3781 attttagaaa tttcgggaat tttttctaaa gaaggtattt taaatagaga gaatcagttg
     3841 aatcaaccta aattgacaat agattgtaat gaatcaggct ctacattgag atttttagta
     3901 cctatatctt taacatttga tggtgtaaaa aggtttatag gtagaggtaa tcttggaaaa
     3961 agaccacttg atacctatta tgagatattt gacagacaga atataaaata tagttacaaa
     4021 gaaaatcagt tagatttgat tatatcaggt aagcttaaac cagatgaatt tagagtaaaa
     4081 ggaaatataa gttctcagtt tatcacaggt ttactgttta tattgcctac gctagaaagt
     4141 gattcaaaga taataattac tactgagcta gaatctaaag gatatttaga tttaactctt
     4201 tcaacgataa aagattttgg ggtagaaatt ataaacaata actataaaga atttattata
     4261 aaaggaaatc aaacgtataa agcgagagat tataaagtag aaggagatta ttctcaagga
     4321 gcattttatt tatcagcaga tgctatagga gaagatatta gcatattaga ccttaaagaa
     4381 gattctcttc aaggggatag tgaagtagtt gaaattttat ctaggatggg aatggaaata
     4441 ttaagagaag gcaacaaaat aaaaggaata acaaatggtt taaatggtac tttgatagat
     4501 gcatctcaat gcccagatat aatacctgta ttatctgtag tagcatcctt aagtataggg
     4561 aggactacaa taattaatgc tggtaggctt agaattaaag aatgtgatag acttcatgca
     4621 ataaatgttg agttaagtaa acttggagca aatatagagg agaaagagga tagtttgata
     4681 atcgaaggag taagcaagtt aaatggtgga gtagaggtgt ggagtcacaa agaccataga
     4741 attgctatga cacttgcaat tgcttcttgt agatgtgata aaccaataat attaaaagat
     4801 tttgagtgtg tatcaaaatc ttatccacat ttttttaaag attttaagat gttaggaggt
     4861 cgaattgatg agtggaatat ggggaaataa tttaaaagta tcaatttttg gagaatctca
     4921 tggaaatgct ataggtataa atatagatgg tcttccttcg gggattgaac ttgaccttga
     4981 taatattgat aaagaaatga agagaagagc acctggaaaa aattctatat ctacttcaag
     5041 aaatgaaagt gatataccag aaattctaag tggatatttt aatggaagaa ctacaggaac
     5101 gccattatgt gcaatcataa gaaatagtga tactcgttca aaagattatg gagagcttaa
     5161 aaatttaatg agaccaggac atgcagattt cacaggtaat gtgagatatt ctggatttaa
     5221 tgactataga gggggaggtc atttttcagg taggataaca gctccattag tattttgtgg
     5281 ggctatatgt aaacaaattt tatcacaaaa aggaatagag ataggagcac acataaaaaa
     5341 aattaaaaat atagaagata tgagttttga ttatgtaaat atttcaaaac aacagttatc
     5401 aaatttgcaa actttggaac tacctttact tgatttatca aaggaagaag ccatgaaaaa
     5461 tacgattata gatgcaaaaa atcaaggaga ttcagttggt ggtataattg agtgtgcagt
     5521 agtaggtatt aatgtagggc ttggaaatcc attttttgac tctgttgaat ctactttatc
     5581 acatttattg ttttctgttc ctgctgtaaa aggtgtagag tttggtttag gttttgaact
     5641 tgcagatatg tatgggtctc aaagtaatga tgaaatgtat tatgaaggaa atcaagttaa
     5701 gagcaaaaca aataacaatg gaggaattat aggagggatt actactggaa tgccaataat
     5761 tttcaaagtg gcaattaagc caactccatc aattagcaga caacaaaaca cagttaatat
     5821 aaaagataaa aaggacgata ttttatatat taaaggtaga catgaccctt gtatagttca
     5881 aagggctata cctgtaatag aagcagtaac agccattgga atttttgatt tgatgaaagg
     5941 acgataataa tggaggattt aacgagatat agaaatgaga ttgacaatat agataaagag
     6001 cttattcaat tatttgaaaa gagaatgaat acagttcttg aaattgccag acataaaatg
     6061 aaaaataaca ctactatact tcaaaaagat agagaagaaa aagtactaag tagagctgta
     6121 gataatttag ataataaagc atattcacaa gaaacggtac agttttttaa ttctataatg
     6181 gaaattagta gaggattaca aaaaagattg atagataatg atggtgagca gaaaatcgag
     6241 tttagtttag attcatcaaa taagaaattt gatttgtcaa atgtaaatag atataagtct
     6301 ttaaaagatg aattaaataa aaaaaatatc ctagtaggat ttccaggaaa atcaggttca
     6361 tttacagaag aagcactaaa taaattcttc aacaaaaaaa tatcaaaaaa acaatttaaa
     6421 gaatttgaag atgttttcat agcacttaaa aataaagaaa taaactatgg aataatcccc
     6481 atagaaaatt catctacagg agctatctct gaaacttatg accttttgag aaagtacggt
     6541 ttttatatag ttggagaaga atgtataaaa atagaccaaa atttaatagg gataaaagac
     6601 acaaagcttg aagatataaa agaaatatat tctcatccac aaggtatagg acaatgtagt
     6661 aaatttttaa aacaaaatag tacatggaaa ttgataccat ttaataacac agcaacaagt
     6721 gcagaacttg taagaaaact tcaagataaa acaaaagcag caattgcaag caaaaaagct
     6781 gcaagtattt atggattaga aataataagt ccttgtataa atgatattgc aaacaactat
     6841 acaagatttg tagtaatcag taatcaaata cacattgaag aagaatcaaa caaaatgagt
     6901 gtagtatttt ctgttgaaca tgaagcagga aaactttata aagtacttgg atactttgct
     6961 aaaaataata taaatatgac aaaaatagaa tcaagaccta tgaaaaatgc ctcatggaga
     7021 tatttctttt atatagattt tgagtgttct atatacaatg ctaaagtgta cgatttatta
     7081 gaattgatag aacataatac agcatatttt aaatttatgg gagtatataa aaataaaact
     7141 gaatagataa gataaatttt tatttaattt ataattatta ggaggtgtaa gtatgaattt
     7201 tttcggtcta gtaggtgaaa aactctctca cagtgtttca cctcaaatcc acaagagagt
     7261 attcgagata ttgaatattg aaagtgctta taaaaacttt gaaatttcaa aagaagatat
     7321 atctaagcta gacggtgcta taaaattatt aggaatacaa ggggtaaatg ttactgtacc
     7381 atataaggag cgtattatga aatatcttga ttttatatca ccagaggcaa aacgtatagg
     7441 agcagttaat acaattttac ttagagaaaa tatgttatat ggttacaata cagattactt
     7501 tggtcttgat agtatgttta aaatggctaa tattgatgtt caaggaaaag tagcagttat
     7561 attaggaact ggaggtgctt ctaaagctgc tcttacatat tttattgatt ctggtataga
     7621 aaagctttat gtttcaacta gaaaaaagga tgataaaaaa ttattaaata gtaaagctat
     7681 attaatagat tatgaagaac ttaaacatat aaaaggagat ataatattaa atgctacacc
     7741 agtaggtatg taccctaatg taggaatttc accagtgtca aaatctatta tacaaaattt
     7801 tgatatatta atagatttaa tttacaatcc tggagaaaca gagtttttaa gaattggtaa
     7861 tagtatgggt aaaaaaactt gtgatggatt atatatgctg gtggggcaag caataaaatc
     7921 tcaggaaatt tggcaagata ctaaaataga taatagtatt ttagatgtta tatataatga
     7981 actaaaatta gaatttttgt aagggagatt gttatgataa ataaaacaaa agaaaaattg
     8041 atattaatag gtatgccagg tagtggaaaa actacaattg gtaaactttt ggccaaagaa
     8101 tataattgta gtttctgtga tatggatgat tatataattc aaatatctca aaaaagtata
     8161 gcagagttgt tttcagaagg agaagatatc tttaggaact atgagactca agcatgtaga
     8221 gaactttcta taagtgataa aacagtgata tctacaggtg gaggtgtaat aaaaaaggat
     8281 gttaatatgg aaatactaaa agaaacaggt ataataattt ttattgatag acctatacaa
     8341 aagattttgg aagatataaa tattaattca agaccattat taaaaaacgg aaaagataga
     8401 ttgtataatc tttataatga gagaataaat ctttataaaa aattttcaga tatagagatt
     8461 ttaaatgata aatcattaaa taatgctgta tataatatta caaatgcagt cagtgaaaat
     8521 tttaaatttg actttaaaga aaaataatct agggggaaat aaaaataatg aatattgtta
     8581 ttgtaggact aggagttata ggaggctctt ttgctaaagc attaaaaaaa gctggatatg
     8641 aaaatgtatt tggagtagat gtagatttag aaactttaaa aaaagcagaa aaagcaaaaa
     8701 tcataaaaaa aggatgtact acaggtaaag agttatttaa aaaagctgat ttaattatat
     8761 tatctatata tcctagatta gttgtagatt ttctaaataa taataaaaac ttttttaaaa
     8821 aagggactat aattactgat actacaggaa taaaagaaac acttatcaat gatgtgttgc
     8881 aaattattcc agatgatata gattttatat ttggacatcc aatggcaggt agggaaaaaa
     8941 agggtattga ttttgcaagt gaacaagttt ttaatggcgc aaattatata attactccaa
     9001 ctggcagaaa caacataaaa aatttagagt tagttgaaaa tttaatttta gagattggat
     9061 ttaaaagagt aaaaaagtta acttcacaaa aacatgatga gattatagct tttacttctc
     9121 agttacctca tgttatggca gttgctctta taaatagtga tgaagaaggt agagatacgg
     9181 gtaaatttat tggagatagt tatagagatt taactagaat tgcaaatatg aatgaagact
     9241 tatggagtga acttttttta ggaaatagag ataatttact aaaagttata gaaaactttg
     9301 aatcagaagt aaatttagta aaagaagcta tatttaacaa tgataaaaat aaattaatag
     9361 agtattttaa aaaatcatca ataagaagag aagctttaga aaaataaata ttaatattta
     9421 agctatatat agatgggtat aaatataagc gatagtgtat ttaattgtat taataaatat
     9481 ggattcaata taattaaata tgctattgat atatttgtac ccttaactat tgttatcttt
     9541 taaatgataa tgatatttat ttaaatactc tttattaaca atttataaaa cttaattgta
     9601 aaaaatactt attaatagta aaataaagtg aatcaattat attataattg ctaaagagag
     9661 tgaggtgtag ctatttgaaa aaaaatagat taatagcaaa gaatatatca atagcattta
     9721 tagtactatt cttttttagt gtatttacat ttttttatgt aggtaatatt aatagagtgt
     9781 tagaatatga aacaaatgac ataataacag ttacaattgc tggatggatt atactttcat
     9841 ttttattttt aggtattata atttacatat tatattcaaa agcaaatagt caaaaaacta
     9901 tagaaaaagt agcatatacg gattttgtaa caggttattc caactggaga aagtttgaat
     9961 tagatgttac aaatttatta aagaaaacat cacaaaataa taaatatgca atggtcatat
    10021 tcgatattga taaatttaaa gcaattaatg atatatatgg ccataaaaag ggaaatttaa
    10081 tattaaaaga tattgcagat actttaaatg aattgactga tatcaatgaa acttttgctc
    10141 gtgtaagtgc tgataatttc aatatattat taacatataa caaaaaagag gatataataa
    10201 atattatcaa aaagattatg gcaaataatg aacttgtaaa cttatctttt ggaatttatg
    10261 aaatcaaaga taaggattta tctgtaagtg tatatagtga tagagcttca cttgcaaaat
    10321 catcaataaa aaataatagt gatgtaaatt ttgcattttt taatgacaaa ttaagagaga
    10381 aattattatt tgaagataag atagaaaaag aaatggaata tgctcttgaa agtgggcaat
    10441 tcgtaatgta tttacaacca aaatacaata ttaaattaga taaattttgt ggaagtgaag
    10501 ccctagtcag atggcaatat acagaaaaag aggtcatata tccaggtgat tttattccta
    10561 tatttgaaaa aaatggattt attagaaaga ttgatatgta catattagaa caagcttgta
    10621 aagaaatccg gtctttattt gataaaggaa tttcaccttt acctatatct gtaaacttct
    10681 caagagttga tttttttaaa aaagatttta ttgaaaacat agtaaatata tgtgatagat
    10741 ataaaatacc atatagttta atagaaattg aaattactga atctagtatg tttggagata
    10801 ctgatactct atttaatgtt agtagaaacc tacaggatat tggctttata gttgcaatgg
    10861 atgattttgg ctctggatat tcttctgtaa atatgcttaa aaatatacca ttaaatgtaa
    10921 taaaattaga tagaggattt tttgttgatg ataaagatgt tgataagagt caaattgtta
    10981 taaaaagcat agtttctctc attaagcaat taggaataag ggttgttgct gaaggaattg
    11041 aaacaagaag tcaaatagaa atgttaaaaa aagcaaattg tgatattgtt caaggatatt
    11101 atttttcaaa accattacca attaaagaat ttgaaaaatt agtctataaa atatagtcag
    11161 cgagtcttaa atgggaggtg gtgattaagt gataagagca aaatcgttaa aaaatgtact
    11221 gagtattgtt ttaatatttt tatttacaat aatttctttt tctgattttg catttaaaac
    11281 taatgtccag gaagaagaaa atagtaaaat taatttaaga gaaatatccc tacaaagttc
    11341 caatgctata aaaataaaat tagatagttc tctatccttt attaaaagtg tggcaaatat
    11401 ggtttcttat tttaatacta tagataactc aaaaatagtt ccattattag ctaatatatc
    11461 aaaagaatct gactttcaaa gaatctctat tgtatctcct gatggaataa gtcataccag
    11521 tgatggcaaa attatcaatg tgtcaaagta tcagtatttt aagaaagcta ttaaaggaga
    11581 aagtaatata tctgaagttg tgaaatctcc aattgatgga aaagatactt ttgttatagc
    11641 tactcctgta tttaataaga ataacaaaat aattggtgtt ttatacggtt cttactacaa
    11701 tgataaatta gctgaattta tagatataac aagttttaat agtgaagggt acatagatat
    11761 atttgaatct agtggtcaat ttgttttaaa atcttcacat acaaacaaac tgattaagtc
    11821 aagtagtaac atatatgata tatttaaaaa agcttctttt gatgatggtt atagctataa
    11881 aaaaatatat gaagatacaa aaaataacaa gagtggcttt acctcatatg aatacaaagg
    11941 agaaaatcgt tatgctactt atacacccat tggtgtaaat gactggtatg taatttctgt
    12001 gataccacaa tatactgtaa gaagaaacat tgataaaata aatcaattag cattttctct
    12061 tattttcaag gtattcttat tgtttattat gcttataata tatattattt ataaaaacaa
    12121 taaatctcag aaagatatac taaaagctaa caaagaaatc tcatctatta caaatagcat
    12181 tcctggagga gtacaaaagt gtaccctggg tgaaacatgc aatattaatt ttttaagtga
    12241 tggatttata gagttgtttg gttatacaag agaagaaata aaaacattgt ttaataatga
    12301 tttttatagt ttaatacata aagatgatgt tgaaagaata aaaaaagaaa tttttaaaca
    12361 actttacaaa aataaaactg ttgaattaga atataggatt ataacaaaaa atggtaatac
    12421 agtatgggtt ttagataaag gggagcttat aacagaacct gatggaaaag aatatttttg
    12481 ttgtgtaact gtagatatta ctagaacaaa acaattagaa caagagatgg aacaaaaaaa
    12541 tctagaactt gaaatgatat cagaaaatgt tttaggtggt ataattataa cagaatttaa
    12601 tgatgatttt actataaaat acgctaactc tggttatctt tcaatgattg gatatacacg
    12661 tgaacaatta gaaaatgaac ttaaatacaa agcttgtaat cttgtttttt atgaggataa
    12721 aaagaaggct cttgaaagta tacaacatca attaaacaca aaaggaatag ctgtatatca
    12781 acatagaata agaagaagaa atggtaatat tatttgggtt tctataaaag gaaggcttat
    12841 tgttgatgat ggtattgaaa aaggtatttg gattctaaat gatataacag atttaaaaaa
    12901 tgcagaacaa aaacttaaaa taaatgaaga aagatttaga attgcattaa agcaaacatc
    12961 aaacactata tttgagtatg atataaaaac aaagtcactt ttaaatacta gtaaagagct
    13021 tgataaatat aatatttata aaattactaa aaacgttcca tattctctag tagaatcagg
    13081 agttattcat aaagatttta gtgatgattt tataaacatg tatgaaaatg taataaatgg
    13141 agcaaaaaaa gcaagttgtg tagttaagat gaaacttcca gatgaaaatt atatatggaa
    13201 taaagttacc ttgactacta tttttgatga agatggtaat cctgtaagag caattggtat
    13261 acaggaagat ataagcaaac aaaaggaagc agagataaga tacaatcaag aaggtaagta
    13321 tcattcagct atattgtcgg aagcaatgat aacttatgaa ataaatgtat caaaggatac
    13381 ttttatcaaa ggaaatgaaa gttggcaaaa accatttgga attaagcctg acaataacta
    13441 ttctcaaatg gtaaaaacta tagcaaatag tattgtacat aaagacaata aagaaacatt
    13501 tttaaataca tttttatgtg aaaacttatt ggatgcatat aaaaataatg tgactgaaat
    13561 aaaattagaa tatcgaagaa tgaatcaaga aaataaaatg atttgggtca tttgtacaat
    13621 gcacttaata aaagattcta ttacgaacga tataaaagca cttgcttatg ttaaagatat
    13681 tgacaagcaa aaaaatgaag aaattagatt aaaattaaaa gctgaaagag atactcttac
    13741 aggattatat aataaaggta ctacagaaaa attgattaat gatttcttga actctgagga
    13801 agcaagctta ggaatgcatg catttttaat tgttgatata gataatttta aatctgtgaa
    13861 tgataattta ggacatgtat ttggagataa agtattattt gaagtttcta aaaacctaaa
    13921 acctatattt agaaaagatg atatagtagg tagaattggt ggagatgaat ttatagtatt
    13981 cttaaaaaat attcagtctt ttgagaatat caaaaagaaa gcagaaaaaa tatgcgatat
    14041 atttagaaat tcttatacag aaaataaaaa gacatataaa atatctggaa cagttggaat
    14101 ttcatttgta ccagaacatg gaactgtatt tgaagaactt tataaaaaag cagacatagc
    14161 actttattat gcaaagagca aaggaaaaga taagtatgca ctttataatg attgtatcta
    14221 ccaaaatgaa cttgaaaata atataagtga taataatata aaattagcag ataaatgtga
    14281 gaaatccaaa ccagtactta aagaaattct tgattctatt gataattata tctatatagt
    14341 gaatccaaat acttatgaac ttctttttgt caataataag gtgtctcgtt tggatttaga
    14401 gataaaatta ggagaaacct gttataaatc actattaaaa tctgatagac catgtaataa
    14461 ttgccctctt aacggattag aagaaaataa gcattcaaaa ttcaaaatag agacaaatac
    14521 atttggtaaa aatatcagta tagcaagttg gattaattgc atagatggag aatacaactg
    14581 tcttatagag tgtatagatt cttctttata caaaaataaa taaaaaagga ctatgaagat
    14641 atgttaaata gtataacttt gatacttggt ataatattta caatttacta tttttatctc
    14701 aaaatagttt ttggaggaat ttcatttagt gagattttct tagttgtagg aattgtacta
    14761 attatctatc aaatatttaa gaaaaaaatt aaagaaaaga aggtttttta tagggtgtta
    14821 aaaataataa tcagcatttt tttaatagta tttgtagtta ctgagagtct aattattttt
    14881 tatcccaaaa atagtttaga atcaaaaagt gattatctct taattttagg agcttctgta
    14941 aaaaagacta caccaagtac aaccctgaag ggtaggttag acactgcatt gaaataccta
    15001 aaaattaatg ataattgtta tgtggtagta tctggtggta aaggaagtgg agaaaaaata
    15061 actgaggcaa aagctatgaa agattatctt ataaaaaatg gtatagataa aaataggata
    15121 atagaagagg ataaatccac gaatacttat gagaatttta aatattcaaa attgaagata
    15181 gaagaacata gtcaaggaaa actaagcgat ttaaaagtaa aaatagtaac aacagatttt
    15241 catgttctca gaagtaaaat attagcatat agaaatggat ataaaaatac gtctttttat
    15301 gctagtaaat caaaactttc atttgtacca acttactata caagagaatt ttttgcatta
    15361 tggaaaacaa tcatttttga tagataataa ataaaaagac tagatataaa ctcatatcta
    15421 gtcttttcta ttcccttaat tctaatccat tttccaactt tacttgttgt agaaatttca
    15481 acattgggat ttacaagtaa ttgcttataa gaagttttat attttacgat tccaaaatat
    15541 aattttcctt cataatccat tataaatccg aatggtctta ctcttggttt attatcttca
    15601 actgtagcaa aatagaatgt tgagttttct ttaaaaaaat tcattatttt ttcatgatta
    15661 aatcctcttt gtatcttata ctaatataat gaatataaat aaaatattca taatactaat
    15721 ataaatcgat ataaaaacaa ttacaatatt atattgatta ttcaaaatta tatataaaat
    15781 tcagaaaaaa attttagttt acataataaa aataaagtca actaaaatta ttgattttag
    15841 attaaagtac cggattaagc atttaagtag attttaagct tgatttgatt agaaaggtac
    15901 aaactatcac cgaaaaaaat taaacccatt taaaaagcaa tataaaagct tgttattttt
    15961 gagaaaaata gaccctctaa actcaattag attaaaaata aaatacagaa aattatatct
    16021 ctaaatatct taaataatta agtttaaatt ttacgatatt ttgtttgatg atttataata
    16081 taaaagcata ctcttaatct tcaaaattta aaaagagtaa aaaataacaa aaaaatcact
    16141 gacatttcac tgacaaattg taattttgtt attataaata cattgaaaat acttgattat
    16201 tgaaaaatgg cataaaataa tacattttat gccatttgct attatttgat agtttttaac
    16261 ttatgacata agatattttt taacatatac aataaaatat aaaaaaattg atattgcaaa
    16321 taaaaaaaaa tacatattag atgttataga aaaaaagcgc acttataggc ttaaaatgtc
    16381 acataaaaga gttttaaaga ataatatatt atgaacacaa attttgtaat cacaaaggag
    16441 tgataatatg gcaacagtat caggacaaat agtttttgat agaaatagaa gtgctaccat
    16501 tgattctgga gactcaggaa tagccaatat accagttgta ttacaagata tagcaactgg
    16561 agttagactt gtggttctca cagatgcatc aggaaattat gtttttacta atgttccaaa
    16621 tggtaactat agaattgtag aagcttttgg aactacagga ggagtaccaa caccaggaaa
    16681 ttttgcaaca gcgatagtag gtccagttcc aattgcagaa atgccaccta ttagttctgt
    16741 gacaagtcct cctattggtt caacaaactt agatggtaca acacctaaca cactattaat
    16801 aacagtagca ggttcagata taacaaatca aaatatatta aatgggcctg tgatttatac
    16861 acctatagag aatatattgg attcttgcgt atctgtatct aatataaacc ttattacaga
    16921 tgcagataac ggaacattcg gtttttttcc accaggtaca ccaagtaata caggacctga
    16981 aatagaacct tacccaaatg tgacgccaga ttttacgtat gttgtaccag acccaactaa
    17041 gtttacacct ttagatggag aatatacagt acaaaatatt atgacaaatg caatgagtaa
    17101 tgttatagga gcttggtggc gtattgctga ccatactaca ggaaatgaaa caggaagaat
    17161 gatggtggta aatggtttta atcctggagc tgtatttttt aaatcaacag tacaagttac
    17221 acctaacact aattatttat ttagcacttg gattttaaac ctttttaagg taacaggata
    17281 tccaccacca cagctaggtg taagaatatt tgaccaaaat aataatatac tatatcaagc
    17341 aacattagga gctcaaattc cagttaatac aaacgcacca gaatggaaag aagtaggtac
    17401 tgtgattaat tctcaaaata ataatcaact tacagtagaa ttttttagtg aaggagaaag
    17461 cgccattgga aatgactatg ctatagatga tgtttcttta agagaaatag ctgttccaac
    17521 ttttactcca ataaaaacaa gtaacacaga aactgctaat gtaggtgata taatcacata
    17581 tacagttaca ttagataata cttgtgatag ccctttaact aatgtatttt ttagggattt
    17641 aattccaaat ggtttagagt ttatacctgg aactgtaacg gttgatggag tatctgtacc
    17701 aggagtagac cccaatacag gtttcccact tccagatatt ggaggaggaa caggggttga
    17761 agtaactttt gatgttgtgg ttgaaagtat tcctaaccca aatcctacta ataatatagc
    17821 tactatagat tactcctata cacctgtaga aggcggtata ccaaatgatt tttcagtaga
    17881 ttctaatcca gttcctgttg aagtaatctc agcagatatt gaagttacaa aattatctga
    17941 acctactata gtgaatccag gagaggaatt aatatacact ataaaagtag taaataatgg
    18001 accattccca tctgaaaatg tggttttaac agatgatgta ccagcttcta ttgtaaatcc
    18061 agagtattca ttagatggag gagtaacttt tcaaccatgg actggaagtt taaatatagg
    18121 gacattagaa gtaggagaga caagagttat tataattagg ggtattgtaa atccatcaac
    18181 agtaggaatt ataacaaata cagctgtagt atcttcaact acagcagacc caaaccttaa
    18241 taataataca tctacaatag agacagaagt gaatttatta gcagatattt tagtaatgaa
    18301 aacagcagaa cctaattcag ctgttccagg tactttattg agatatacta ttcaagtaga
    18361 aaacttagga ccagcaaatg ctgaaaatgt aatattaaat gatgatattc cagcttctat
    18421 tataaatcca gaatattcat tggatggagg agcaagtttt caaccatgga atggaagtct
    18481 taatatagga actttaaact ctggaatttc attaacagtt ttaattcaag gaacagttag
    18541 tttaaattct tcagaatata tagtaaatac agcaacagtt tcttcaacta cacctgaccc
    18601 agatttaagc aacaatatat ctaccattat tacaccagtt aacccacaag caggtatttc
    18661 tattataaaa gtagctgacg aggatgtagc tgttccagga gaagaatttg tatatacaat
    18721 tgaaattttt aatgaaggac catctaatgc tacgaatgta gttttaacag atgatatacc
    18781 agatgttatt ttaaatccag aatattcatt ggatggagga gcaactttcc aaccatggac
    18841 tggaagtctt aatataggta cagttgcacc agggcaatta ataagaatta tcataaaggg
    18901 attagtaagt tcaactgcta caggagatat tactaataca gcagaagtaa gtgcagatgt
    18961 accagaacca gttacggatt catcaacagt gacaactcct atagttccat cagcagatat
    19021 agaagtaata aaaactagca atatggatac agcagttcca ggagaaactt tcagttatac
    19081 tataacagtt acaaatttag gaccatctgc tgcacaaagt atcgttttga cagatgatat
    19141 accagatgtt attttaaatc cagagtattc attggatgga ggagtaactt tccaaccatg
    19201 gaatggaaat cttagtatag gcactttgga tgctggagaa ataagaagta ttataataag
    19261 aggaacagtg agccagacag cagtaggtac aattataaat acagcaacaa taagttcaac
    19321 tacacctgac ccaaatccag ataataatac ttcaacagat gaaacagata taagtccatt
    19381 agcagatatt tctgtgataa aaggcaacga accagttgct ataccagggg gaagatttat
    19441 ttatggaatt gaaattgcaa atgcaggacc atcttttgca gagaacgtaa ctttaacaga
    19501 taatatacca gcttctattt taaatccaga gtactcaata gataatggag tgactttcca
    19561 accatggaat ggaagcctta atataggtac tttagatgct ggagagataa gaaatatcat
    19621 aataagggga acagtaagtc aaacagcaat aggcacaatt ataaatacag caacagtaag
    19681 ttcaactaca cctgacccaa atctaaataa caatatttca acatcagaag cagaggtaag
    19741 ttcatcagca gatatttctg tagtaaaacg aagcaatcag actgttgttg taccaggaga
    19801 tgtactagac tatacaattg aggtgagaaa tgcaggaccg tctactgctc agaacgtaac
    19861 tttaacagat aatataccag cttctatttt aagtccagaa tattcaatag ataatggagt
    19921 gacttttcaa ccatggaatg gaagtcttag tataggcact ttagatgctg gagagataag
    19981 aaatattata ataaggggaa tagtaagtca aacagcaata ggtacaatta taaatacagc
    20041 aacagtaagt tcaactacac ctgacccaaa tccagataat aatacttcaa catcagaaat
    20101 agatataagt tcatcagcag atatttctgt aataaaatta gcaaataaaa cagaagcatg
    20161 tgtaggagag caaatagatt ttgtaatagt agtatcaaat gcaggaccag aaaatgctca
    20221 aaatgttact ttaatagaca atgtttcaga taagcttaag aaagcagtat tttcacttga
    20281 tagaggtgta agtttccaac catggactgg aagtcttaat ataggtacac taccagctgg
    20341 aactttaaga gtgattatat taagaggaat tataaaatca acatgtcttg atagattaac
    20401 aaatattgca gaaattactt caacaacacc agattcaaac cttaacaata atatatcaag
    20461 agtgcaagta gaaataaaac aatgttgttg taatgattgc tgttgtaaca actgtagtaa
    20521 ttgttgttgc aatgattgtg gttgtaaaga ttgttgctgt aattgttgta aaaatgactt
    20581 ttggtgttga ttttataaaa taattttgta taccttaaat taaactgaaa taaaaaataa
    20641 attcacatat gagtaaaata ttttataaat attaataaat tttctatctt tgatatggac
    20701 cacattcgta atactagtta ttaaatgaag tggtcctttt taattcatta aaattattta
    20761 ttctaattaa attttaaa
//
LOCUS       DAMJVK010000069        20726 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.070, whole genome shotgun sequence.
ACCESSION   DAMJVK010000069 DAMJVK010000000
VERSION     DAMJVK010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20726
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.070"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            370..681
                     /gene="rpsJ"
                     /locus_tag="PC414_002583"
     CDS             370..681
                     /gene="rpsJ"
                     /locus_tag="PC414_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HDF2464971.1"
                     /translation="MAKNEKIRIRLKSYDHKLLDFSAGKIVETAKKAGSQVSGPVPLP
                     TEKQVVTILRAVHKYKDSREQFEIRTHKRLIDIANPTPKTVDSLMRLDLPAGVDIEIK
                     L"
     gene            773..1402
                     /gene="rplC"
                     /locus_tag="PC414_002584"
     CDS             773..1402
                     /gene="rplC"
                     /locus_tag="PC414_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HDF2464972.1"
                     /translation="MKGILGKKVGMTQIFTDKGVVIPVTAVEAGPMVVTQIKTVDKDG
                     YNAIQIGFEDAKEKALNKPKKGHLAAANVLKKHLKEFRVDSVEGYTVGQEIKADVFEA
                     GAKIDVTGISKGKGFQGPIKRHGQSRGPETHGSRYHRRPGSMGACSYPGRVFKNKKLA
                     GHMGSVKVTVQNLEVVKVDADKNLILVKGAIPGAKGSVVTIKEAIKVSK"
     gene            1432..2055
                     /gene="rplD"
                     /locus_tag="PC414_002585"
     CDS             1432..2055
                     /gene="rplD"
                     /locus_tag="PC414_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001127258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HDF2464973.1"
                     /translation="MPKLNVLNVSGQNVGEIELSDSIFGVEVNGHVLYEVVKNQLANK
                     RQGTQSAKTRAEVRGGGRKPWKQKGTGRARQGSTRSVQWVGGGVAFAPKPRSYKYTLP
                     KKVRRLAMKSALSSKVQNSEVIVLDALNMDAPKTKEFAQILNNINAAKKALVVIADKN
                     DNVIKSARNIEGVQTALVNTMNVYDILKYDSFIITTDAVKKVEEVYA"
     gene            2055..2345
                     /gene="rplW"
                     /locus_tag="PC414_002586"
     CDS             2055..2345
                     /gene="rplW"
                     /locus_tag="PC414_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HDF2464974.1"
                     /translation="MTNPHDVIIRPVVTEHSMAEMGEKKYTFVVAKDANKTEIKKAVE
                     KVFGVSVDKVNTLNYDGKVKRMGRTQGRTSSFKKAVVKLTADSKEIEFFQGM"
     gene            2375..3205
                     /gene="rplB"
                     /locus_tag="PC414_002587"
     CDS             2375..3205
                     /gene="rplB"
                     /locus_tag="PC414_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HDF2464975.1"
                     /translation="MAIKKFRPTSPALRQMTVLVSDEITCNQPEKSLLVNLKKNAGRN
                     VHGRITVRHRGGGQKRKYRIIDFKRDKDGIPAKVATIEYDPNRTANIALLNYADGEKR
                     YILAPVGINVGDTILSGLGADIKPGNCLALKDMPVGTIIHNIELKPGKGAQLVRSAGV
                     SAQLMAKEGKNALLRLPSGEMRLVSINCKATIGQVGNIEHGNVVIGKAGRKRHMGIRP
                     TVRGSVMNPNDHPHGGGEGRSPIGRPSPVTPWGKPALGYKTRKKNKASNKLIVSRRTK
                     "
     gene            3240..3521
                     /gene="rpsS"
                     /locus_tag="PC414_002588"
     CDS             3240..3521
                     /gene="rpsS"
                     /locus_tag="PC414_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDF2464976.1"
                     /translation="MSRSTKKGPFVHARLLKKIEAMNASGNKEVIKTWSRSSTVFPQM
                     VENTIAVHDGRKHVPVYITEDMVGHKLGEFVPTRTFKGHKDDEKSNKRK"
     gene            3553..3888
                     /gene="rplV"
                     /locus_tag="PC414_002589"
     CDS             3553..3888
                     /gene="rplV"
                     /locus_tag="PC414_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDF2464977.1"
                     /translation="MEAKATAKYVRVSPRKAGQICDLVRGKNVDEALAILKFTPRGAA
                     SIIAKVVKSAKANAENNHEMDTEKLYIASIVANQGPTMKRFMPRAMGRATTIRKRTSH
                     IEVVVKEKK"
     gene            3911..4726
                     /gene="rpsC"
                     /locus_tag="PC414_002590"
     CDS             3911..4726
                     /gene="rpsC"
                     /locus_tag="PC414_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HDF2464978.1"
                     /translation="MGQKVNPHGLRVGVIKDWDSRWFATDKKEFGNLLLEDHNIRKFL
                     KKRLYSAGVAKIEIERSANKIKMDLHVAKPGVVIGRAGAGIEALKAELEKMTKKTIIV
                     NIVEVRSTDKNAQLVAENIALAIERRVAFRRAMKQAIQRAMKSGAKGIKVSASGRLGG
                     AEMARTEGYSEGNVPLQTLRADIDYGFAEADTTYGKIGIKVWICNGEVLPTRDGVNPR
                     EESRKSDRRDNKRDNRRNDRRGNDRRGNDNRGNYRGQRPQGGSRPQRTENKGN"
     gene            4762..5193
                     /gene="rplP"
                     /locus_tag="PC414_002591"
     CDS             4762..5193
                     /gene="rplP"
                     /locus_tag="PC414_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HDF2464979.1"
                     /translation="MLMPKRVKRRRVHRGSMAGQAHKGNKVTYGEFGLVALEASWITS
                     NQIEAARIAMTRYIKRGGKVWIKIFPHKPVTRKPAETRMGAGKGSPEYWVAVVKPGRV
                     MFELAGVSEDKAREAMRLAAHKLPIKCKFVKKEDLEVKGGE"
     gene            5195..5398
                     /gene="rpmC"
                     /locus_tag="PC414_002592"
     CDS             5195..5398
                     /gene="rpmC"
                     /locus_tag="PC414_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HDF2464980.1"
                     /translation="MKAKELRDLTSEELMNKLNDFKSELFSLRFQLATGQLENTARIK
                     FVKKDIAKVKTVLAERKLYETRA"
     gene            5422..5676
                     /gene="rpsQ"
                     /locus_tag="PC414_002593"
     CDS             5422..5676
                     /gene="rpsQ"
                     /locus_tag="PC414_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HDF2464981.1"
                     /translation="MERGRRKVRIGRVVSDKMDKTIVVAVEEFVRHPLYNKRVKRTKK
                     FKAHDEKNICNIGDRVKIMETRPLSKDKRFRLVEVVEKVK"
     gene            5702..6070
                     /gene="rplN"
                     /locus_tag="PC414_002594"
     CDS             5702..6070
                     /gene="rplN"
                     /locus_tag="PC414_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007885948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDF2464982.1"
                     /translation="MIQQESRLRVADNSGAKELLCIRVLGGSKRRYGNIGDVIVATVK
                     SATPGGVVKKGKVVKAVIVRSKQGVRRNDGSYISFDENAAVIIKDDKTPVGTRIFGPV
                     ARELRDNEFMKIVSLAPEVL"
     gene            6094..6399
                     /gene="rplX"
                     /locus_tag="PC414_002595"
     CDS             6094..6399
                     /gene="rplX"
                     /locus_tag="PC414_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDF2464983.1"
                     /translation="MRVKKGDTVVVIAGKDKGKKGSVLKVYPKTSKVLVEGVNVITKH
                     QKPSAMNQQGGIINKEAPIHISNVMPFDPETGKGVRVRYEVKDGNKVRVSAKSGKEL"
     gene            6429..6971
                     /gene="rplE"
                     /locus_tag="PC414_002596"
     CDS             6429..6971
                     /gene="rplE"
                     /locus_tag="PC414_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDF2464984.1"
                     /translation="MASRLQEKYMKEVAPALMEKFGYKNVMEIPKLNKIVINMGIGDA
                     RENPKGLEKGVEELEMISGQKPVITKARKSVANFKLREGMPIGTKVTLRADKMFYFMD
                     KLVSVSLPRVRDFRGVNPNAFDGRGNYALGVKEQLIFPEIEYDKIDKVRGMDIIFVTT
                     AKTDEEARELLKLLGMPFSK"
     gene            6989..7174
                     /locus_tag="PC414_002597"
     CDS             6989..7174
                     /locus_tag="PC414_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HDF2464985.1"
                     /translation="MARKAMVVKQQRKQKYATREYTRCTICGRPHSVLKKFGICRICF
                     RELAYKGQIPGVRKASW"
     gene            7207..7605
                     /gene="rpsH"
                     /locus_tag="PC414_002598"
     CDS             7207..7605
                     /gene="rpsH"
                     /locus_tag="PC414_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDF2464986.1"
                     /translation="MTMTDPIADMLTRIRNANVVKHETVDVPASNMKKELARILLEEG
                     FIRGYDVIEDGKQGIIRIQLKYGQEGERVITGLKKISKPGMRVYAANHEIPKVLNGLG
                     ISVISTSKGILTDKQARKENVGGEVICYVW"
     gene            7636..8178
                     /gene="rplF"
                     /locus_tag="PC414_002599"
     CDS             7636..8178
                     /gene="rplF"
                     /locus_tag="PC414_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDF2464987.1"
                     /translation="MSRIGVKPIIIPAGVEVTIAEGNLVTVKGPKGTLTKQLSAELNI
                     KKEENTIMVERPTDNKKHRSLHGLTRTLLDNMVVGVNTGFEKKLELKGVGYRAQKQGK
                     KLVMNLGFSHPVEMEDPEGITVEAPNQTELIVKGIDKQLVGNYAAKIRAWRKPEPYKG
                     KGIKYVDEVIRRKEGKTGKK"
     gene            8214..8582
                     /gene="rplR"
                     /locus_tag="PC414_002600"
     CDS             8214..8582
                     /gene="rplR"
                     /locus_tag="PC414_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDF2464988.1"
                     /translation="MLKKADKNANRLQRHKRVRRKISGTSQRPRLCVFRSANNIYAQI
                     IDDTKRVTLVAASSLEAEVKSAVNHTGNKEAAKKVGELVAKKAVEKGITEVVFDRGGY
                     LYHGRIQELAEGAREAGLKF"
     gene            8603..9112
                     /gene="rpsE"
                     /locus_tag="PC414_002601"
     CDS             8603..9112
                     /gene="rpsE"
                     /locus_tag="PC414_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011109776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDF2464989.1"
                     /translation="MLRRKPIDAGQLDLQEKVVEVRRVTKVVKGGRNFRFAALVVVGD
                     ENGHVGIGAGKAMEVPDAIKKAVEDAKKNLIVVPIVGTTIPHEVRGHFGAGNILIMPA
                     VEGTGVIAGGPARAVLELAGLKDVRAKSLGSNNPRNMVNATIEGLNSLKTVEDIAKLR
                     GKKVEELLG"
     gene            9127..9312
                     /gene="rpmD"
                     /locus_tag="PC414_002602"
     CDS             9127..9312
                     /gene="rpmD"
                     /locus_tag="PC414_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDF2464990.1"
                     /translation="MAKLQIKLVRSVIGTTPNQKKNVEALGLRKREQVVVKEDNAQTR
                     GMINKVSHLLEVTEIAE"
     gene            9345..9788
                     /gene="rplO"
                     /locus_tag="PC414_002603"
     CDS             9345..9788
                     /gene="rplO"
                     /locus_tag="PC414_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDF2464991.1"
                     /translation="MKLHELKPAEGAVRAKRRLGRGTATGQGKTAGRGQKGQWSRSGG
                     GVRVGFEGGQMPLARRLPKRGFNNIFKKVYTEVNVEVLNRFENGTEITAELLKSTKTI
                     SKIGKDGIKILGEGNLEKALTVKAAKFTASAQEKIEKAGGKAELV"
     gene            9832..11100
                     /gene="secY"
                     /locus_tag="PC414_002604"
     CDS             9832..11100
                     /gene="secY"
                     /locus_tag="PC414_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDF2464992.1"
                     /translation="MLSKLKQAWKIKAVRKKVMYTLMMIVIFRIGTTIPVPGIDTSII
                     QKMVGGNSLLSLYNMFTGGAFSNFSLFALGISPYITASIIIQLLTVGFESLAELQKSG
                     EEGKKKINKYTKYTALALAVVQALGITLGIVRSALISNSVFFITTVVITLVSASMLVM
                     WIGDKITEKGIGNGSSVIIFAGIISRIPTDVIKISQQVKSGEVAPWVIVILAVVILLT
                     VTGVTFIQEATRKIPVQYAKRVVGRKMYGGQSSHIPMKVNQSGVMPIIFASSLLAFPQ
                     TIAMFMGPNAQAFVQKYLSMATEQGFWTYRSIEILLIIFFSYFYTTVSFNTEDISKNM
                     KNNGGFIPGIRPGEPTMDYLNRILTRLTLAGATFLAIIAMVPALTTHYMKVNMSLAGT
                     SLLIVVGVALELKRQLESNLVMRSYQGFLK"
     gene            11117..11767
                     /locus_tag="PC414_002605"
     CDS             11117..11767
                     /locus_tag="PC414_002605"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDF2464993.1"
                     /translation="MRIILLGPPGAGKGTQAAGIVEKYNIPHISTGDIFRKNIKEGTE
                     LGKKAKEYMDQGLLVPDELTVGLVTDRISQEDCKNGFMLDGFPRNVAQGEHLDIFLKN
                     AGISLDKVVNIEVDKSILVSRAVGRRICKSCGATYHVEFNPPKVEGVCDVCQGELYQR
                     ADDNEETVSKRIQVYLDETKPLVDYYSKQGIIADIKGDQAIDKVFEDIVAALGSGK"
     gene            11768..12514
                     /gene="map"
                     /locus_tag="PC414_002606"
     CDS             11768..12514
                     /gene="map"
                     /locus_tag="PC414_002606"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDF2464994.1"
                     /translation="MIILKSKKEIELLREAGKIVADTHEVLRKAISPGISTLELDKIA
                     EENIRKYNAEPSFKGYGGFPGSICASINREVVHGIPGETILQEGDIVSLDIGAYYKGY
                     HGDSAKTHGVGMISEEDRKLIEVTRESFYEGIKFAKLGYRLSDISHAVQTHVEKHGFS
                     VVRDLVGHGVGANLHEDPQVPNYGLPGKGPRLREGMVIAIEPMVNAGRYHVKTLSDGW
                     TTVTIDGKKSAHYEHTIAITEHEPLILTKL"
     gene            12536..12805
                     /locus_tag="PC414_002607"
     CDS             12536..12805
                     /locus_tag="PC414_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW domain-containing protein"
                     /protein_id="HDF2464995.1"
                     /translation="MLSDNLSIGQVVKVSLGRDKGNLFFVVKIINNEYVLIADGKKRK
                     LDKPKLKKVKHLKKYNFINDEVRKRVVSGQEITDSFLRAELTKLN"
     gene            12843..13061
                     /gene="infA"
                     /locus_tag="PC414_002608"
     CDS             12843..13061
                     /gene="infA"
                     /locus_tag="PC414_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HDF2464996.1"
                     /translation="MAKKDVIELEGTVSEALPNAMFKVKLENGHEILCHISGKLRMNF
                     IRILEGDKVNVELSPYDLTRGRITWRKK"
     gene            13097..13210
                     /gene="rpmJ"
                     /locus_tag="PC414_002609"
     CDS             13097..13210
                     /gene="rpmJ"
                     /locus_tag="PC414_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HDF2464997.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQKQG"
     gene            13363..13734
                     /gene="rpsM"
                     /locus_tag="PC414_002610"
     CDS             13363..13734
                     /gene="rpsM"
                     /locus_tag="PC414_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HDF2464998.1"
                     /translation="MARIAGVDLPREKRAEIGLTYIYGIGKATANEILAKAEINPDTR
                     IKDLSEDQVNELRKVIDDDFLVEGDLRREIALNIKRLRDIKCYRGIRHAKGLPLRGQR
                     TKTNARTRKGPRKTVSRKKKK"
     gene            13770..14168
                     /gene="rpsK"
                     /locus_tag="PC414_002611"
     CDS             13770..14168
                     /gene="rpsK"
                     /locus_tag="PC414_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HDF2464999.1"
                     /translation="MAKPKKKVTRIRRRERKNIERGHAHIQSTFNNTIITLTDVHGNA
                     ISWASSGQLGFKGSRKSTPFASQMAAETAAKAAMEHGLKSVEVFVKGPGSGREAAIRA
                     LQATGLEVTMIKDVTPIPHNGCRPPKRRRV"
     gene            14200..14823
                     /gene="rpsD"
                     /locus_tag="PC414_002612"
     CDS             14200..14823
                     /gene="rpsD"
                     /locus_tag="PC414_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HDF2465000.1"
                     /translation="MARYTGASCRQCRREGMKLFLKGDRCYTDKCAIVKRNYAPGQHG
                     QGRKKVSNYGLQLREKQKVKRIYGVLETQFRNLYERAENMPGKAGENLLSLLERRLDN
                     VVYRMGLASSRKEARQLVTHGHFTLNGNKVDIPSLIVKVGDVIEVKEKSRSSAKFKNL
                     VEVNSRIAPKWLEANVEGMTAKVVGVPTREDIDLEIAEHLIIELYSK"
     gene            14901..15848
                     /locus_tag="PC414_002613"
     CDS             14901..15848
                     /locus_tag="PC414_002613"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HDF2465001.1"
                     /translation="MIEIEKPKVDIVELSEDYRYGKFVIEPLERGYGITIGNALRRIL
                     LSSLPGVAVNAIKIDGVLHEFSTIPGVKEDVTEIILTLKELSATIDGEGSRTLKIEAQ
                     GPCSITGADIICPPDVEILSKDLAIATLDDNAKLNMEIFVDKGRGYVSAEENKTENVP
                     IGVLPVDSIYTPVEKVSYHVENTRVGQKTDYDKLVLEVWTNGSINPQEGISLAAKVLV
                     EHLNLFIDLTEHVSSVEIMVEKEEDQKEKVLEMTIEELDLSVRSYNCLKRAGINTVEE
                     LANKSEDDMMKVRNLGKKSLEEVIQKLEELGLGLKPSEE"
     gene            15869..16210
                     /gene="rplQ"
                     /locus_tag="PC414_002614"
     CDS             15869..16210
                     /gene="rplQ"
                     /locus_tag="PC414_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003357477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HDF2465002.1"
                     /translation="MAKYRKLGRETAHRNLMLRNLVTCLLRSGRIETTVTRAKETRRM
                     AEKMITLAKRGDLHARRQVLAYVMDETVVNNLFTDLAPKYAERNGGYTRIIKIGPRKG
                     DAAEMAFIELV"
     gene            16330..17163
                     /locus_tag="PC414_002615"
     CDS             16330..17163
                     /locus_tag="PC414_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HDF2465003.1"
                     /translation="MDNIVKVNNISFEYITDEAKLKAIDNLSLDVKKGEFVAIIGHNG
                     SGKSTLSKNLNAILMPTEGNILIDDMDTKEEERLWDIRQTAGMVFQNPDNQIVATIVE
                     EDVAFGPENLGIEPKEIRRIVEESLKSVGMYDLRDRQPHLLSGGQKQRVAIAGIIAMR
                     PKCIIFDEATAMLDPSGRKEVMKTIKRLNKEENITVIHITHFMEEAVEADRVVVMEKG
                     KKILEGTPREVFSKIKMLKEIGLDVPCMTELSSLLIEEGINISSDILTVDEMVMELCQ
                     L"
     gene            17151..18017
                     /locus_tag="PC414_002616"
     CDS             17151..18017
                     /locus_tag="PC414_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HDF2465004.1"
                     /translation="MSIIVKNLTHIYNEGMPFASKALDDVSFEIKDRDFVGLIGHTGS
                     GKSTLIQHLNGLLKPSSGEIFINDFNITDKNLNLTEIRKRVGVVFQYPEYQLFEETID
                     KDIAFGPSNLGLEESEIHNRVKASMEAVGLDYEGFKDKSPFELSGGQKRRVAIAGVIA
                     MNPEVLILDEPTAGLDPGGRDEIFNLIKDLHEKKNMTIILSSHSMDDMAKLAKTLIVM
                     NHGSVEFMGTPREVFKSNASKLKDIGLDIPQVLELALKLREKGFDISEDILTLEEAKQ
                     EILKVVRGRGLC"
     gene            18011..18814
                     /locus_tag="PC414_002617"
     CDS             18011..18814
                     /locus_tag="PC414_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDF2465005.1"
                     /translation="MLKDITIGQYYPTSSAIHKLDPRIKLVATIVFMVSIFVVNKFWP
                     YIVVLLCLLAMIKLANIPVKYIVKGVKPLKWIILFTFLINIFFLPGDEIWSFGFLAIT
                     KQGLRQAIFMAIRLIFLVVGTSLLTLTTSPIELTDGIERLLNPFKKIGLPVHELAMMM
                     TIALRFIPTLLDETDKIMKAQMSRGADFESKNLINRAKNLVPLLVPLFVSAFRRADEL
                     AMAMEARCYRGGHNRTKMRESVISRRDYMACVFQVVYLGAIIATRFIAI"
     gene            18846..19577
                     /gene="truA"
                     /locus_tag="PC414_002618"
     CDS             18846..19577
                     /gene="truA"
                     /locus_tag="PC414_002618"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDF2465006.1"
                     /translation="MRNIKIKIQYNGKNYCGWQKQPDSLGIQGTIERAIYDITKEETS
                     LIGSGRTDSGVHAIGQIANFKINSGISIESIPMALNAKLPKDISVIEACEVNDDFHSR
                     YSAKGKTYKYLVYNSKFRNPILSEISYQVKYELDFDKMCSEAKSLLGTHDFKGFMSSG
                     SSVKDTVRTIYDIDISKKDDLITFEISGNGFLYNMVRIIVGTLVDMGRGRINEPFLDI
                     IQSKTRSRCGHTAPAQGLFLKKVHY"
     gene            19697..20128
                     /gene="rplM"
                     /locus_tag="PC414_002619"
     CDS             19697..20128
                     /gene="rplM"
                     /locus_tag="PC414_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDF2465007.1"
                     /translation="MKSYIAKPADVQRKWYLVDAEGKTLGRLATEIATVLRGKHKPTF
                     TPHVDGGDFVVVVNAEKIVLSGKKLDQKYYRYHTGYVGGLKEISYRDMMDKKPEEVIS
                     HAVSGMLPKNKLRSRMMTRLRVFAGAEHTHAAQNPEVLNFK"
     gene            20157..20549
                     /gene="rpsI"
                     /locus_tag="PC414_002620"
     CDS             20157..20549
                     /gene="rpsI"
                     /locus_tag="PC414_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDF2465008.1"
                     /translation="MANVQYYGTGRRKSSVARVRLVAGEGNILVNGRALENYFNYETL
                     IRDVKQPLVLTGNENKYDVIVKVEGGGFTGQAGAIRHGISRALLKADLDLRPALKKEG
                     FLTRDARMKERKKYGLKAARRAPQFSKR"
BASE COUNT         8278 a         2447 c         4321 g         5680 t
ORIGIN      
        1 ctttactaaa ctaatggatt taatataaaa aaaagagggg gtaactcctc tcttttttgt
       61 tttttgttga caagtagcgc taaagcatat atactaaact agtgccttga gacagattaa
      121 caatcaagtt taaggttatg atgttgtaat agaaaataaa attgaaaaaa acagaaaaaa
      181 aacttgaaaa aatgtaacag atgatataaa atataatagt gtgcttgaga gattaagaaa
      241 ataataatta aatcctagtg aaatacgaac tgatatcgat ttaaaagtta caacacaccc
      301 ggagcagtgt atcggtattg gttgtacgca acttgaaaat taaacacgtg ataaaggagg
      361 gaaaacaaaa tggctaagaa tgaaaaaata agaataagat taaaatcata tgatcacaaa
      421 ttattagatt tttcagcagg taaaatagtt gaaactgcta aaaaggctgg atcacaagtt
      481 tcaggacctg tgcctctacc aacagaaaaa caagttgtaa ctatattaag agctgtacat
      541 aagtacaaag actctagaga acaatttgaa ataagaactc ataaaagact aatcgacata
      601 gctaatccaa cacctaagac agttgactca ttaatgagat tagacttacc agcaggtgta
      661 gatatagaaa taaaattata agaaccccta agatattatc ctatagatga ttatcttaga
      721 atgattactc taaaagatgt aatccgctgt aagaattata ggaggtgcta atatgaaagg
      781 aatattaggc aaaaaagttg gtatgactca aatattcact gataaaggtg tagttatacc
      841 tgtaacagct gttgaagcag ggcctatggt cgttactcaa ataaaaacag ttgataaaga
      901 tggatacaat gctatacaaa taggttttga agatgctaaa gaaaaagctt taaacaaacc
      961 taaaaaagga catttagctg ctgctaatgt tttaaagaaa catttaaaag aatttagagt
     1021 agattctgta gaaggataca cagtaggaca agaaataaaa gctgatgtat ttgaagcagg
     1081 tgcaaaaata gatgttactg gaataagtaa aggtaaggga ttccaaggtc caataaaaag
     1141 acatggacaa agtagaggtc ctgaaactca cggttctaga taccacagaa gaccaggttc
     1201 aatgggagct tgttcttacc caggtagagt atttaaaaac aaaaaattag ctggacatat
     1261 gggaagcgta aaggtaacag ttcaaaactt agaggttgtt aaagtagatg ctgacaagaa
     1321 ccttatatta gttaaaggag ctataccagg agctaaaggt tcagtagtaa ctataaaaga
     1381 agctataaag gtttctaaat aatgactaac ctagagaaag gaggaataac aatgccaaaa
     1441 ttaaatgtac taaatgttag tggacaaaac gttggagaaa tagaattatc agattctata
     1501 tttggcgtag aagttaatgg acatgtttta tacgaagttg taaaaaatca attagcaaat
     1561 aagagacaag gaactcaatc tgctaaaact agagcagaag ttagaggtgg cggaagaaaa
     1621 ccttggaaac aaaaaggaac aggtagagca agacaaggtt ctacaagatc tgtacaatgg
     1681 gtaggtggag gagttgcttt tgcacctaaa ccaagaagct acaaatatac attacctaag
     1741 aaggtaagaa gattagctat gaagagtgct ttatcttcaa aagttcaaaa tagtgaagtt
     1801 atagtattag atgcattaaa catggatgct cctaagacta aagaatttgc tcaaatatta
     1861 aacaatataa atgctgctaa aaaagcttta gtagttatag ctgacaaaaa cgataacgta
     1921 ataaaatcag ctagaaatat agaaggtgtt caaactgcat tagttaatac tatgaatgta
     1981 tatgatatat taaaatacga ttcatttata ataactacag atgctgttaa aaaagtggag
     2041 gaggtgtacg cataatgact aatccacatg atgtaataat aagaccagtt gtaactgagc
     2101 acagtatggc tgaaatgggt gaaaaaaaat acacttttgt tgtggctaaa gatgcgaaca
     2161 aaactgaaat aaaaaaagca gtagaaaaag tattcggagt tagtgttgat aaagtaaata
     2221 ctttaaacta cgatggaaaa gttaaaagaa tgggtagaac tcaaggaaga acatcaagct
     2281 ttaagaaagc agtagttaaa ttaactgctg atagtaaaga aatagaattc ttccaaggaa
     2341 tgtagtatta taacaaacga aggagggaaa acagatggct ataaaaaagt ttagaccaac
     2401 ttctcctgcc ttaagacaaa tgacagtttt agtttctgat gaaataacat gtaatcaacc
     2461 agaaaaatct cttttagtta acttaaagaa aaatgctggt agaaacgtac atggtagaat
     2521 aactgttcgt catagaggtg gaggacaaaa aagaaaatat agaataatag attttaaaag
     2581 agataaagat ggtatacctg ctaaggttgc tactatagaa tatgatccaa acagaactgc
     2641 aaatatcgca ttattaaact atgctgatgg agaaaaaaga tatatattag ctccagttgg
     2701 aattaacgtt ggagatacta tattatcagg attaggtgct gatataaaac caggaaactg
     2761 tttagcatta aaagatatgc cagttggtac tatcattcat aatatagaat taaagccagg
     2821 aaaaggagct caattagtta gatctgctgg agtttctgca caattaatgg ctaaagaagg
     2881 aaaaaatgct ttattaagat taccatcagg tgagatgaga ttagtaagca taaactgtaa
     2941 agctactata ggtcaagttg gaaatataga gcatggtaac gtagttatcg gtaaagcagg
     3001 tagaaaaaga catatgggaa taagacctac tgtaagaggt tctgtaatga accctaatga
     3061 ccatccacac ggtggagggg aaggtagatc tccaataggt agaccatctc ctgttactcc
     3121 atggggtaaa ccagctcttg gatacaaaac tagaaagaaa aataaagctt caaataaact
     3181 aatagtatca agaagaacta agtagtaaat ttatttttgc tcggaaagga ggaatataaa
     3241 tgtcaagatc aactaaaaaa gggccttttg tccatgcaag acttttaaag aagatagaag
     3301 ctatgaatgc tagtggaaat aaagaagtta taaaaacttg gtcaagatct tcaactgtat
     3361 ttccacaaat ggtagaaaac actatagctg ttcatgatgg aagaaaacat gttccagtat
     3421 atataacaga agatatggtt ggacataaat taggtgagtt cgttcctact agaactttca
     3481 aaggacataa ggatgacgaa aaatctaata agagaaaata attaaattat gactaagaaa
     3541 ggaggaataa caatggaagc aaaagctact gctaaatacg tacgtgtatc accaagaaaa
     3601 gcaggccaaa tatgtgacct tgttagagga aagaatgttg atgaagcatt agcaatatta
     3661 aagtttactc caagaggagc agcttcaata atagctaagg ttgtaaaatc agctaaagct
     3721 aacgcagaaa acaatcacga aatggatact gaaaaattat atatagcatc aatagttgct
     3781 aatcaaggac caacaatgaa gagattcatg cctagagcta tgggtcgtgc aactacaata
     3841 agaaaaagaa cttctcatat agaggttgtt gttaaagaaa aaaaataatt aatagatagg
     3901 agggaaagta atgggtcaaa aggttaatcc acacggctta agggtcggtg ttataaaaga
     3961 ttgggactca agatggttcg ctactgataa aaaagagttc ggaaacttat tattagaaga
     4021 ccataatata cgtaaattct taaagaaaag attatactca gctggagttg ctaagataga
     4081 aatagaaaga tcagctaaca aaataaaaat ggacttacac gttgctaagc ctggtgtagt
     4141 tataggaaga gctggtgcag gaatcgaagc attaaaagct gaattagaaa aaatgacaaa
     4201 gaaaactata atagttaata tagtagaagt aagaagtaca gataaaaatg ctcaattagt
     4261 agctgagaat atagctttag ctatagagag aagggttgct ttcagaagag caatgaaaca
     4321 agctatacaa agagcaatga aatctggtgc taaagggata aaagtttctg cttcaggaag
     4381 attaggtgga gctgaaatgg ctagaactga aggttatagt gaaggaaatg tgccactaca
     4441 aactttaaga gcagatatag attatggttt cgctgaagct gatacaactt atggaaaaat
     4501 cggtataaaa gtttggatat gtaatggtga agttttacca actagagacg gtgtaaatcc
     4561 aagagaagaa agcagaaaga gcgatagaag agacaacaag agagataata gaagaaacga
     4621 tagaagagga aatgacagaa gaggaaatga taacagagga aactacagag gacaaagacc
     4681 tcaaggtgga tcaagacctc aaagaactga aaacaaagga aattaataat agttaggttc
     4741 aaggaaggag gaaaaatact catgttaatg ccaaaaagag taaagcgtcg tagagttcat
     4801 agaggaagca tggctgggca agctcataag ggtaataaag ttacttatgg tgagtttgga
     4861 ttagtagcat tagaagcttc ttggataact tctaatcaaa tagaagctgc cagaatcgcg
     4921 atgactagat atataaaaag agggggaaag gtttggataa aaatattccc tcataaacca
     4981 gtaacaagaa aaccagcaga gactcgtatg ggtgcaggga aaggttctcc agaatattgg
     5041 gtagctgtag ttaagccagg aagagttatg tttgaattag caggtgtttc tgaagataaa
     5101 gcaagagaag ctatgagact tgctgcacat aaattaccaa tcaaatgtaa atttgttaaa
     5161 aaagaagatt tagaagtaaa gggtggtgaa taggatgaaa gctaaagaat taagagattt
     5221 aacaagcgaa gagctaatga acaaattaaa cgacttcaaa agtgaattat ttagcttaag
     5281 attccaatta gctactggtc aattagaaaa tacagctaga ataaagtttg ttaagaagga
     5341 tatagcaaaa gttaaaactg ttcttgctga aagaaagtta tacgaaacta gagcttaatt
     5401 tggaaaggag gctttttaaa catggaaaga ggaagaagaa aagttagaat aggccgtgtt
     5461 gttagtgata agatggataa aacaatagta gtagctgttg aagaattcgt acgtcatcca
     5521 ctatataata aacgtgttaa gagaactaag aaattcaagg ctcatgatga aaagaatata
     5581 tgtaacatcg gagatagagt taaaataatg gaaactagac cattatctaa agataagaga
     5641 ttcagactag ttgaagttgt tgagaaagtt aagtagtttt tgaagaagga gggattacga
     5701 tatgatacaa caagaatcac gtctaagagt tgctgataac tcaggagcta aagaactttt
     5761 atgtatacgt gttctaggcg gaagtaaaag aagatatggt aacataggcg acgttatagt
     5821 tgctactgtt aaaagtgcaa cacctggtgg agttgtaaaa aaaggtaagg ttgttaaagc
     5881 tgttatagta agaagcaaac aaggcgtaag acgtaatgac ggaagctata tatcttttga
     5941 tgaaaatgct gctgttataa taaaggacga taaaactcca gtaggaactc gtatattcgg
     6001 gcctgttgct agagagttaa gagacaatga attcatgaaa atagtttctc ttgctccaga
     6061 agtactataa ttaaggaggt gcaataggac atgatgcgtg ttaaaaaagg tgacactgtt
     6121 gtagttatag caggtaaaga taaaggtaaa aaaggttcag ttttaaaggt atatcctaaa
     6181 actagcaaag tgttagttga aggtgtaaat gtaataacta aacatcaaaa accaagtgct
     6241 atgaaccaac aaggtggaat aataaataaa gaagccccaa tacacatatc taatgtaatg
     6301 ccatttgatc ctgaaacagg aaaaggcgtt agagtaagat atgaagtaaa agatgggaac
     6361 aaagtaagag tatcagcaaa gagcggaaaa gaattataat aaatattgct tgaaaggagg
     6421 gaccttaaat ggcttctaga ttacaagaaa aatacatgaa agaagttgct cctgctttaa
     6481 tggagaaatt tggatacaaa aacgtaatgg agatacctaa gttaaataaa atagttatta
     6541 acatgggtat aggtgacgct agagaaaatc caaaaggatt agaaaaaggc gttgaagaat
     6601 tagaaatgat atcaggacaa aagcctgtta taactaaagc tagaaaatct gttgctaact
     6661 tcaaattaag agaaggaatg ccaataggaa caaaagttac attaagagct gacaaaatgt
     6721 tctactttat ggacaaatta gtttcagttt ctttaccaag agttagagac tttagaggag
     6781 ttaaccctaa tgcttttgat ggtagaggaa attatgcttt aggagttaaa gaacaattaa
     6841 tattccctga aatagaatat gataaaatag ataaagtaag aggaatggat ataatatttg
     6901 ttactacagc aaaaactgac gaagaagctc gtgaattatt aaaattatta ggaatgccat
     6961 tttctaagta agcaaaggag ggattcccgt ggctagaaaa gcgatggttg ttaagcaaca
     7021 aagaaagcaa aagtacgcta ctagagaata cactagatgt actatatgcg gaagaccgca
     7081 ttcagttttg aaaaaatttg gtatatgccg tatatgcttt agagaattag cttataaagg
     7141 tcaaatacct ggtgttagaa aagcaagctg gtagaagctt gattagaatg gaaggagggc
     7201 aacaaaatga caatgacaga tccaattgca gatatgctta ctcgtataag aaatgctaat
     7261 gttgttaagc atgaaactgt agatgttcca gcttctaata tgaagaaaga attagctaga
     7321 atcttattag aagaaggttt cataagaggt tatgatgtta tagaagatgg aaaacaaggt
     7381 atcataagaa tacaattaaa gtacggacaa gaaggcgaaa gagttataac aggtcttaag
     7441 aaaatatcta agcctggaat gagagtttac gctgctaacc atgagatacc aaaagtatta
     7501 aacggattag gaatatcagt tatatctact tcaaaaggaa tattaactga caagcaagct
     7561 agaaaagaaa atgttggtgg agaagtaatc tgctacgttt ggtaattaat atgaacaatg
     7621 taaggaggtg cgaccatgtc aagaataggt gttaagccaa taataatacc agcaggtgtt
     7681 gaagttacta tagctgaagg aaatttagtt acagtaaaag gtccaaaagg aactttaact
     7741 aaacaattaa gtgctgagtt aaacataaaa aaagaagaaa atacaataat ggttgaaaga
     7801 ccaactgata ataaaaaaca tagatcttta cacggactaa ctagaacttt attagataat
     7861 atggttgtag gtgtaaatac tggtttcgaa aagaaattag aattaaaagg tgttggatat
     7921 agagctcaaa aacaaggaaa gaaattagtt atgaacttag gtttctctca tccagttgag
     7981 atggaagatc cagaaggaat aactgttgaa gctccaaacc aaacagagtt aatagtaaaa
     8041 ggtatagaca agcaattagt agggaattat gctgctaaga taagagcttg gagaaagcca
     8101 gagccataca aaggtaaagg tataaaatac gttgatgaag taatcagacg taaagaagga
     8161 aaaactggta aaaaataata ccaaaactta aacgctagaa aggagtgagc tagatgttaa
     8221 aaaaggctga taaaaatgct aatagacttc aaagacataa gagagttcgt agaaaaataa
     8281 gcggtacttc tcaaagacca agattatgtg tatttagaag tgctaacaat atatatgctc
     8341 aaataataga tgacacaaaa agagtaactc tagttgctgc atcttcttta gaagcagaag
     8401 ttaagagtgc tgtcaatcat acaggaaaca aggaagcagc taagaaagtt ggagaattag
     8461 ttgctaaaaa agctgttgaa aaaggaatca ctgaagttgt atttgacaga ggtggatact
     8521 tatatcatgg aagaattcaa gaattagctg aaggtgctag agaagctggt cttaagttct
     8581 aatacgaagg agggaaaaat acatgctacg tcgtaagccg atagatgcag gacaacttga
     8641 cttacaagaa aaagttgttg aagtaagacg tgttactaag gttgtaaaag gtggtagaaa
     8701 tttcagattt gcagcattag tagttgttgg agatgaaaac ggacacgttg gtataggtgc
     8761 tgggaaagca atggaagtac cagacgctat aaaaaaagca gttgaagatg ctaagaaaaa
     8821 tctaatagta gtgccaatag ttgggactac aattcctcac gaagtacgcg gacactttgg
     8881 tgctggaaat atattaataa tgcctgctgt agaaggtaca ggagtaatag ctgggggacc
     8941 tgctagagcc gtacttgagc tagctggact aaaagacgtt agagctaaat ctttagggtc
     9001 taacaatcca agaaatatgg taaatgctac aatagaagga cttaattctc taaaaacagt
     9061 tgaagatata gctaaactta gaggtaaaaa agttgaagaa cttctagggt aaggaggtag
     9121 tgtaaaatgg ctaaattaca aataaagtta gttagaagtg ttataggaac tactcctaac
     9181 caaaaaaaga atgttgaagc gttaggatta agaaaaagag aacaagttgt tgtaaaagaa
     9241 gacaatgctc aaacaagagg tatgataaat aaagtaagtc acttattaga agtaactgaa
     9301 atagctgaat aatttttata aagtagtgca aggaggtgca atccatgaag ttacatgagt
     9361 taaaacctgc tgaaggtgca gtaagagcta agagaagatt aggtagaggt actgcaactg
     9421 gacaaggtaa aactgcagga cgtggacaaa aaggtcaatg gtctcgttct ggtggtggag
     9481 taagagtagg atttgaaggt ggacaaatgc cactagctag aagacttcct aagagaggtt
     9541 tcaataacat atttaagaaa gtttacacag aagttaatgt tgaagtttta aacagatttg
     9601 aaaatggaac agaaataact gcagaattat taaaatctac taaaactata agcaaaatag
     9661 gtaaagacgg aattaaaatc ttaggtgaag gtaatttaga gaaagcttta actgttaaag
     9721 ctgctaaatt tacagcttca gctcaagaaa aaatagaaaa agctggtgga aaagcagagt
     9781 tagtataata acctgctaat ctcctagcca gtacaggcgg ggtgaattag cgtgctgtca
     9841 aaattaaaac aagcttggaa gattaaagca gtaagaaaaa aagtaatgta tacacttatg
     9901 atgatcgtga tattcagaat aggtactact ataccagttc caggcataga cactagtatt
     9961 atacaaaaga tggtaggcgg caatagtcta ctttctctgt ataatatgtt tactggtgga
    10021 gcatttagta acttttcact atttgcttta ggtataagtc catatatcac tgcatcaatc
    10081 ataatacaac ttctaacagt aggttttgaa agtttagcag aacttcaaaa atctggtgaa
    10141 gaaggaaaga aaaaaatcaa taagtacact aaatatacag cactagctct agcagttgta
    10201 caagctttgg gaattacatt aggaattgta agaagtgctt taatatcaaa tagtgtgttt
    10261 tttatcacaa ctgttgtaat aacattagtt tcagcaagta tgttagtaat gtggattggg
    10321 gacaaaatta ctgaaaaagg aatcggtaat ggtagttcag taataatatt tgcaggtata
    10381 atatcaagaa tacctacaga tgttattaaa atatcgcaac aagttaaatc tggcgaagtt
    10441 gcaccttggg ttatagtgat tctagctgta gttatattac ttacagtaac aggagttaca
    10501 tttatacaag aagcaactag aaagatacct gtacaatatg ctaaaagagt tgttggaaga
    10561 aaaatgtatg gaggacaaag ttctcatata ccaatgaaag ttaaccagtc aggagttatg
    10621 cctataattt ttgctagttc acttttagct ttccctcaaa caatagctat gtttatgggt
    10681 ccaaatgctc aggcttttgt tcaaaaatac ttaagtatgg caacagaaca aggattttgg
    10741 acatatagat caattgagat tctacttata attttcttct catattttta tacaactgta
    10801 tcatttaata cagaagacat atctaaaaat atgaaaaata atggtggatt tataccaggt
    10861 ataagaccag gtgagcctac tatggattat ttaaatagaa ttttaactag attaacttta
    10921 gctggagcta ctttcttagc aataattgct atggtgccag ctcttacaac acattatatg
    10981 aaagtaaata tgagtctagc tgggacttca ttgctaatcg ttgttggagt tgctttggag
    11041 cttaagagac agttagagtc aaacttagtt atgagaagct atcaaggttt cttaaaataa
    11101 aatggagatg gataatatga gaataatatt acttggacct ccaggtgctg gtaaaggtac
    11161 tcaggcggca ggaatagtag aaaaatacaa tatacctcat atatcaacag gagatatatt
    11221 cagaaagaat ataaaagagg gaacagaact tggaaaaaaa gctaaagaat acatggacca
    11281 aggtttatta gtaccagatg agttaactgt aggtttagtt actgatagaa tatctcaaga
    11341 agattgtaaa aatggattta tgttagatgg atttccaaga aatgtagcac aaggagaaca
    11401 tttagatatc ttcttaaaaa atgctggtat atcactagat aaagttgtca atattgaagt
    11461 tgataagagt atattagtgt ctagagcagt tggtagaaga atatgtaagt cttgtggagc
    11521 tacttaccat gttgagttta atcctcctaa agtagaaggt gtatgtgatg tatgccaagg
    11581 agaattatat caaagagctg atgataatga agaaactgta tctaagagaa tacaagttta
    11641 tctagatgaa actaagcctt tagtagatta ttatagcaaa caaggtataa tagcagatat
    11701 aaaaggtgat caagcaatag ataaagtatt tgaagatatt gtcgcagcct taggaagtgg
    11761 aaaataaatg attatcttga aatcaaaaaa agaaatagaa cttttaagag aagcaggtaa
    11821 aatagttgct gacactcatg aagttttaag aaaagctatt tctccgggaa tatctacttt
    11881 agaattagat aaaatagctg aagagaatat aagaaaatac aatgcagaac cttcttttaa
    11941 aggatatgga ggttttccag gaagtatatg tgcttctatc aatagagagg tagtacatgg
    12001 tatcccagga gaaactatat tacaagaagg tgacattgta agtttggata taggagccta
    12061 ttataaagga tatcatggtg attctgcgaa aactcacgga gtaggcatga tatctgaaga
    12121 agataggaaa ttgatagaag taactagaga aagcttctat gaaggaataa aatttgctaa
    12181 attaggatat agactttcag atatctcaca cgcagtacaa acacacgttg agaaacatgg
    12241 tttctcggta gttagagatt tagtgggtca tggagttgga gcaaatcttc atgaagaccc
    12301 acaagtacct aattatggac tcccaggaaa aggaccaaga cttagagaag gaatggttat
    12361 tgctatagag ccaatggtta atgcaggtcg ttatcatgta aagactctat cagatgggtg
    12421 gacgactgtt acaatagatg gaaaaaaatc agctcattat gagcatacga tagcaattac
    12481 tgaacatgaa cctttgatat tgacaaagtt gtaatttaaa aagtaggtga aaaaagtgct
    12541 atcagataat ttaagtatag gtcaagttgt taaagtttca ttaggacgag ataaaggaaa
    12601 tttattcttt gtagtcaaaa taattaataa tgaatatgta ttaatagctg atggaaaaaa
    12661 gagaaaactt gacaaaccta agttaaagaa agtgaagcac ttaaaaaaat ataattttat
    12721 taatgatgaa gttagaaaaa gagtagtatc tggacaagag attacagatt cttttttaag
    12781 agctgaactt actaagttaa attagataat ttaacttatt gaaatggagg gctggttagt
    12841 taatggccaa aaaagatgtt atagaattag aaggtacagt ttcagaagct ttacctaatg
    12901 ctatgttcaa agttaaacta gaaaatggac atgagatatt atgccacatt tctggaaagc
    12961 taagaatgaa cttcataaga attcttgaag gtgacaaggt gaatgttgaa ctttctccat
    13021 atgaccttac aagaggaaga attacttggc gtaagaagta gactatatta atatttagtc
    13081 taaggaggga ttaataatga aggttagacc atcagtaaaa ccaatatgtg aaaaatgtaa
    13141 agtaataaaa agaaaaggaa aagtaatggt tatctgtgaa aatccaaagc ataagcaaaa
    13201 gcaaggataa gaaattatta ctagcatttt gttaaatttt atagtataat actatattgt
    13261 gatgttaaca aaagaacgtt aaaatcgttg aacaatctgg cgtaggattg gcatatgatg
    13321 tgaagtttta aacactaaaa actatgtgta ggaggtgcga tcatggcaag aatagctggg
    13381 gtagatttac ctagagaaaa aagagcggag ataggcttaa cttatatata tggtataggt
    13441 aaagcaactg ctaacgaaat attagctaag gctgagataa atccagatac tagaataaaa
    13501 gatttatcag aagatcaagt taatgagtta agaaaagtta tagatgacga tttcttagtt
    13561 gaaggtgact taagaagaga aattgcttta aacataaaga gattaagaga tataaaatgt
    13621 tatagaggaa taagacacgc taaaggtctt ccattaagag gacaaagaac taagactaat
    13681 gctagaacta gaaaaggtcc tagaaaaaca gtatctcgta aaaagaaaaa ataaataaat
    13741 aataactaga tgaaggaggg aaaaggaaaa tggctaaacc aaaaaagaaa gttacacgta
    13801 ttagaagaag agaacgtaaa aatatagaac gtggtcatgc tcatatacaa tcaactttca
    13861 ataatacaat aataacttta actgacgttc acggaaatgc tatatcttgg gcaagttctg
    13921 gacaattagg attcaaagga tcaagaaaat caactccatt tgcatctcaa atggctgctg
    13981 aaacagctgc aaaagctgcg atggaacacg gactaaaaag tgttgaggta ttcgtaaagg
    14041 ggccaggttc aggaagagaa gctgcaataa gagctttaca agcaactgga ctagaagtaa
    14101 ctatgataaa agacgttact ccaatcccac ataacggatg cagaccacca aagagaagaa
    14161 gagtgtaatt aataagagta caataggagg tgtagataaa tggcaagata tacaggtgca
    14221 tcatgtagac aatgccgtag agagggaatg aaattattcc ttaaaggtga tagatgttat
    14281 acagacaaat gtgctatagt aaaaagaaac tatgctccag gtcaacatgg ccaaggaaga
    14341 aagaaagttt ctaactatgg attacaatta agagaaaaac aaaaagttaa aagaatatat
    14401 ggagttttag agactcaatt tagaaattta tatgaacgtg ctgaaaatat gcctggtaaa
    14461 gcaggtgaga acttattaag tttattagag agaagattag ataatgtagt ttacagaatg
    14521 ggactagcat cttctagaaa agaagctaga caattagtga ctcatggtca tttcacttta
    14581 aatggaaata aagtagacat accttcatta atagttaagg ttggagatgt tatagaagtt
    14641 aaagagaaat caagatcttc tgcaaaattc aaaaatttag tagaagttaa ctcaagaata
    14701 gctcctaagt ggttagaagc taatgtagaa ggaatgacag caaaagtagt tggtgttcca
    14761 acaagagaag atatagatct tgagatagca gagcacttaa tcatagaact ttactctaaa
    14821 taataagcac acttttaaaa attattttaa aattttgttt accctcagtg gattactgaa
    14881 ttttaaggag ggttttgtcc atgatagaaa tagaaaagcc aaaagtagat atagttgagc
    14941 ttagcgaaga ctatagatat ggtaagtttg ttatagagcc tctagaaaga ggatatggaa
    15001 taactatagg taacgcatta agaagaatat tattatcatc attaccaggt gtagcagtga
    15061 atgctataaa aatagatgga gttcttcatg aattttcaac aatacctggt gttaaagaag
    15121 atgttactga gataatatta actttaaaag agctttcagc aactatagat ggtgaaggaa
    15181 gtagaacact taaaatagaa gctcaaggac catgctctat cacaggtgca gatataatct
    15241 gtcctccaga tgttgaaata ttaagtaaag atttagcaat agctacatta gatgataatg
    15301 ctaaacttaa tatggagata tttgtagata aaggtagagg ttatgtttct gctgaagaaa
    15361 ataaaacaga gaatgttcca ataggtgttt tacctgtgga ttcaatatat actcctgttg
    15421 agaaggttag ttatcatgtg gaaaacacaa gagttggtca aaaaacagat tatgataaat
    15481 tagtattaga agtttggact aatggtagta taaatcctca agagggaata tcattagctg
    15541 caaaggtatt agttgagcat ttaaacttat ttatagactt aactgaacat gtaagtagtg
    15601 ttgaaatcat ggtagaaaaa gaagaagatc aaaaagaaaa agttcttgaa atgactatag
    15661 aagaattaga tttatcagtt agatcatata actgtttaaa gagagcggga attaatacag
    15721 ttgaagaatt agctaataaa tctgaggatg atatgatgaa ggttagaaac ctaggtaaga
    15781 agtcattaga agaggtaata caaaaactag aagaacttgg attgggatta aaaccaagtg
    15841 aagaatagcg agcaaaggag ggataagaat ggctaagtac cgtaaattag gacgtgaaac
    15901 agctcacaga aaccttatgt taagaaactt agtaacttgc ttactaagaa gtggaagaat
    15961 agaaactaca gtgactagag cgaaagaaac tcgtagaatg gctgaaaaga tgataactct
    16021 tgctaagaga ggagatcttc atgctagaag acaagtttta gcttatgtta tggatgaaac
    16081 agtagttaat aacttattca cagatttagc tccaaaatat gctgagagaa atggtggata
    16141 cactagaata ataaaaatag ggccaagaaa aggcgatgct gctgaaatgg cttttataga
    16201 attagtatag taaggaaagg attaggcttg atggcttaat ctttttttta tttttttgac
    16261 taaggtatga tataataatg tgtaaaatga tttttataag ttattgctta ctaaaaggag
    16321 tatgagttaa tggataatat agtaaaggta aataatattt cgtttgagta tattacagat
    16381 gaagcaaaac ttaaagcaat agataattta agcctagatg ttaaaaaagg agaatttgtt
    16441 gcgataatag gacataatgg ttctggtaag tctactttat ctaaaaactt aaatgctatt
    16501 cttatgccta ctgaaggaaa tattcttatt gatgatatgg atactaaaga agaagaaaga
    16561 ttatgggata taagacaaac tgcaggcatg gtatttcaaa atccagataa tcaaatagtt
    16621 gctactatag tagaagaaga cgttgcattt ggacctgaga acttaggtat agaaccaaaa
    16681 gaaataagac gaattgtaga ggagtcttta aaaagtgtag gtatgtatga tttaagagat
    16741 agacagcctc atttgttatc aggaggtcaa aagcaaagag ttgcaatagc aggaattatt
    16801 gctatgaggc ctaaatgtat aatatttgat gaagcaacag caatgttaga tccatctgga
    16861 agaaaagaag ttatgaaaac aataaaaaga cttaataagg aagaaaatat aactgttata
    16921 catataacgc actttatgga agaagcagta gaagctgata gagttgtagt tatggaaaaa
    16981 ggaaagaaaa tcttagaggg aactcctaga gaagtattca gtaaaataaa gatgttaaag
    17041 gaaatcggtt tagatgtacc ttgtatgaca gagttatcta gtttattaat agaagaaggt
    17101 ataaatatta gtagtgatat attaactgtg gatgagatgg tgatggaatt atgtcaatta
    17161 tagtaaagaa tttaacacat atatataatg aagggatgcc ttttgcaagt aaggcacttg
    17221 atgatgtttc ttttgagatt aaagatagag attttgttgg tcttataggt catacaggct
    17281 caggaaaatc tacattaata caacatttaa atggattact aaagccttcc tcaggagaaa
    17341 tatttataaa tgattttaat ataacagata aaaacttgaa tttaactgag attagaaaaa
    17401 gagtaggggt tgtatttcaa tatccagaat accaattgtt tgaagaaacg attgataaag
    17461 atatagcttt tggaccctct aatttaggct tagaggagtc tgagatacat aacagggtaa
    17521 aagcatcaat ggaagctgta ggacttgatt atgagggatt taaagacaaa tctccttttg
    17581 aattatctgg gggacaaaag cgtagagtag caattgctgg agttatagcc atgaacccag
    17641 aagttctcat attggatgaa ccaactgctg gtcttgaccc tggtggaaga gatgaaattt
    17701 ttaacttaat aaaagactta catgaaaaaa agaatatgac tataatacta tcatcacata
    17761 gtatggatga tatggcaaag ttagcaaaaa ctttaattgt tatgaatcat ggtagcgtag
    17821 aatttatggg aactcctaga gaagtattta aatcaaatgc tagtaaatta aaagatatag
    17881 gattagatat tccacaagtt ttagagcttg cattgaaatt gagagaaaaa ggctttgaca
    17941 tcagtgagga tatattgact ttagaagaag caaaacagga aatattaaaa gttgtgagag
    18001 gacgaggatt atgttaaaag atataactat agggcaatac tacccaacaa gttctgctat
    18061 tcataaatta gatccaagga taaagcttgt agcgacaatt gtatttatgg tatctatatt
    18121 tgtagtaaat aaattttggc cttatatagt agttttatta tgtttattag ctatgataaa
    18181 attggctaat ataccagtca aatatattgt taagggtgtt aaacctctta aatggattat
    18241 tttgtttaca tttcttataa atattttttt cttaccaggt gatgaaatat ggtcatttgg
    18301 atttttagct attacaaaac agggtctaag acaagcaatt tttatggcaa taagattaat
    18361 atttttagta gttggtacat cattgcttac tttaacaact tcaccaatag aattaactga
    18421 tggaatagag agattgttga atccatttaa gaaaattggt ttgccagttc atgaacttgc
    18481 aatgatgatg acaattgctt tacgttttat tcctacattg ttagatgaga cagacaaaat
    18541 aatgaaggct cagatgtcta gaggtgcaga ttttgaaagt aagaatctta taaatagagc
    18601 aaagaattta gtaccacttt tggtaccttt atttgtaagt gcatttagaa gagctgatga
    18661 attggctatg gccatggagg ccagatgtta cagaggtgga cataatagga ctaagatgag
    18721 agagtctgtt atatcaagaa gggattatat ggcatgtgta tttcaggttg tttatctggg
    18781 agcaattata gctactagat ttatagcaat ttaattaata agttatagat ataatgaggt
    18841 aaagtatgag aaatataaaa attaaaatcc aatacaatgg taagaattat tgtggatggc
    18901 aaaagcaacc tgattcttta gggatacaag gtacaattga aagggctata tatgatataa
    18961 caaaagaaga aaccagtctt atagggtctg gtagaactga ttcaggagtt catgctattg
    19021 gtcagattgc aaattttaaa attaattcag gtataagtat agaaagtata ccaatggcac
    19081 taaatgccaa gttgccaaaa gacatatcag ttatagaagc atgtgaagta aatgatgatt
    19141 ttcactccag atacagtgca aaaggtaaaa catataaata tttagtatat aatagcaagt
    19201 ttagaaatcc aatacttagt gagatatcat atcaagtaaa atatgaactt gattttgata
    19261 aaatgtgttc agaagcaaaa agtttattag gaacacatga ttttaaaggt tttatgagtt
    19321 ctggttcatc tgtaaaagat acagttagaa cgatatatga tattgatata agtaaaaaag
    19381 atgacttgat tacttttgag attagtggta atggtttttt atataacatg gtgaggatta
    19441 ttgttggaac acttgttgat atgggtcgtg gcagaattaa cgaaccattt ttagatataa
    19501 tacaatcaaa aactaggtca agatgtggac atacagctcc agcacaagga ttatttctca
    19561 aaaaagttca ttattaactt gacacaccag gctacatgta ttaaaatata tgagaatgtg
    19621 ttaataagtc cacttgcccc ggacttgaca taaacggtaa aaaaatttta tgaaaagtag
    19681 ttaaggaggg acagttatga aaagttatat agctaagcca gctgatgtac aaagaaaatg
    19741 gtacttagtt gatgctgaag gaaaaacatt aggtcgttta gcaacagaaa ttgcgacagt
    19801 attgagaggt aaacataaac caacatttac tcctcacgtt gatggtggag attttgttgt
    19861 tgtagtaaat gcagaaaaaa tagttttaag tggtaagaaa ttagatcaaa aatattacag
    19921 ataccatact ggttatgtag gtggattaaa agaaatatct tatagagata tgatggataa
    19981 gaaaccagaa gaagttatat cacatgctgt aagcggtatg ttacctaaaa ataaattaag
    20041 aagcagaatg atgactagat taagagtatt tgcaggtgct gagcacactc atgcagctca
    20101 aaatccagaa gttttaaact ttaaataaga ttaataggtg aaaggaggag tttataatgg
    20161 ctaacgttca atattatgga actggaagaa gaaaaagttc tgttgctaga gtaagactag
    20221 ttgcaggtga aggaaatata ttagtaaatg gaagagcatt agaaaattat tttaattatg
    20281 agacattaat aagagatgtt aaacaaccat tagttttaac tggtaatgaa aataaatatg
    20341 atgttatagt aaaagttgaa ggtggaggat tcactggaca agctggagct ataagacatg
    20401 gaatatctag agctttatta aaagctgatt tagatttaag acctgcttta aagaaagaag
    20461 gtttcttaac tagagatgca agaatgaagg aaagaaagaa atacggatta aaagcagcaa
    20521 gaagagctcc acaattctca aaaagataat tacttatttt tttggaaaag caaaatcctt
    20581 tatttactgt tattcagaaa gtattggtat tttaaaaatg ttgggttaag ttaattattg
    20641 atatataata aaataataag atatcttatt aggatgtttt tcattaagaa gaacatcctt
    20701 tttattttga ctatttaata aaaaac
//
LOCUS       DAMJVK010000070        20623 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.071, whole genome shotgun sequence.
ACCESSION   DAMJVK010000070 DAMJVK010000000
VERSION     DAMJVK010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20623
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.071"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            96..1019
                     /locus_tag="PC414_002621"
     CDS             96..1019
                     /locus_tag="PC414_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2465009.1"
                     /translation="MEIRVLRYFLAIAREESISGAATFLHITQPTLSRQIMELEEELG
                     VKLLVRGNRKITLTNEGRLLHKRAEEIISLVDKVELEFDNPNEIISGDIYIGSGETEA
                     MRLIARTAHHFHQKYPNVRYHLFSGNADDVTEKLDKGLLDFGILIEPANIQKYDYAQI
                     PATDVWGLLMRKDSALASQSTINPKDLCNIPLIISRQTLVDKAISKWLQDSNEKLNII
                     ATYNLVYNASLLVEEGLGYALCLDRLVNTTENSNLCFRPLSPPLEAKLNIIWKKHQIF
                     SKAAEKFLLQIQKDFSSIMLDTSKLDTPKEK"
     gene            complement(1191..2333)
                     /locus_tag="PC414_002622"
     CDS             complement(1191..2333)
                     /locus_tag="PC414_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDF2465010.1"
                     /translation="MDFKIEKYLLKKAEELAFITIKHGGEFKLKSYEVPKGGLDVPIK
                     NEVLVKGIKEKTAQDKLNSMSIADAMIYIIGIDSKFKNNAEYEKFLNALSKDIDLDLK
                     SYMGYMSRKYFEIGEHTDSLIYIKAFITMYPDDLDAMYNYAIVCQEIAKQYQKDMDDK
                     AMNAFLLEAMAKLEKVIDVDENFALGYYHLGYHYYNQGQYLKTKLTWEEALRLGLDAD
                     LVAEVQENLGKMDFKVQYEEGYTLVFQGKFKEGLEKLLPLEEEHMDWWNLLFMIALGY
                     KGMGEIEQAKMYLEKILIIKPNQVDTIVELGLCEAYKNNLDKAIEYFEQAAKIKEDPE
                     ILCNLGMAYLNNGDIDDATYYIERAYELNPQDEITIACLRELRINK"
     gene            complement(2351..4105)
                     /locus_tag="PC414_002623"
     CDS             complement(2351..4105)
                     /locus_tag="PC414_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HDF2465011.1"
                     /translation="MKILLTTLNSKFIHTNLAIRYLKEFVRDLIEVNMKEYTINNDLD
                     YILKDIYKNEYDIILFSTYIWNVGDIVKLCDNLKKIRPNTKIALGGPEVSYDSYEAMK
                     KYDFVDYILYGEGELIFRDLVLHLQGKMEINDVNGLVYRQGNEIIVNKPMELLQDLDE
                     IPSPYENLNPKEYENRIVYYESSRGCPFNCQYCLSSTIPGLRYFSLDRIKSDLKALID
                     ARVSQIKFIDRTFNANRKVAMEIMDFLMKNDNGYTTYHFEVTAYLIDDKMLEFLADCK
                     EGLFQFEIGVQSTNEKTLDAVGRRDDFKKLSHVVQTVASYRNIHQHLDLIAGLPYEDY
                     KSFENSFNDVFNLGIEHLQLGFLKMIKGTGMRKVADEHGFKYKDYAPYEFLYNNYISY
                     EETLKLKDIEDILERYYNSKNFVLSMRYIIGRFYKKSPFKFFEVFAKYFDENGYFDLA
                     QGKNQLYKILMDFYNEKINIDNDVFNDILKYDYISLGKTSNIPQFFNKLDVDDFKNRC
                     HVFLQDNDNLSTYLPSFVDKPAKHIIKYVHFEPFRFNVVDLKNDINTEIREVENVVLF
                     EYDDKKIFEKSKTHKVEI"
     gene            4241..4447
                     /locus_tag="PC414_002624"
     CDS             4241..4447
                     /locus_tag="PC414_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465012.1"
                     /translation="MELIEKVSINSLSKRDLLLIIKSLEYTNENTQISDFIDLRNNIV
                     KELCFLTDTKEKDFLDYLEKNSTL"
     gene            complement(4452..5375)
                     /locus_tag="PC414_002625"
     CDS             complement(4452..5375)
                     /locus_tag="PC414_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465013.1"
                     /translation="MNDELLKKVYTSSFSSASRALKDLTIINTKSLIDDKTQRCVYID
                     ENRLRDELIYYRFYGEKIGNINFLNILLPLILSNTNIQKSEDEVIKLIKKYVIYFKKE
                     ELLFDYLLGSVVYNSIMHNLINNSKIEYVELLQSIKDKIIGFSIELDKSDVVKFQMAR
                     IKAIQLIDKYIDLKSEDYDEESILLNVLNVLYDVYMEDRTVENEGINSIKKSILSILG
                     EDSKLNEDNIDFIFSMSEYVVKLRKYKIGVKAYNKSIDPRSLIRLEEGNTIIDPIFNQ
                     ITVMSKTFNDNILSIKINSKSGIYILKFKKV"
     gene            complement(5629..7329)
                     /locus_tag="PC414_002626"
     CDS             complement(5629..7329)
                     /locus_tag="PC414_002626"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDF2465014.1"
                     /translation="MQDFKVAISNCLKEKIEDLSKEEIEALIEVPPNKDMGDYAFPCF
                     KLAKVFRKAPNMIASELAESIEPSGEITKVIQLGGYVNFFVNKSQLAETVIKKVLDEK
                     ENYGHSDFGKDKTVIVEYSSPNIAKPFHIGHIRTTVIGNALYKIYDSQGYKTIRINHL
                     GDYGTQFGKLIVAFKKWGEKEVVESNPIPELLKLYVRFHDEAEQHPEMEDEARAWFNK
                     LENGDEEAQELWQWFRNESLKEFNRVYKLLDIEFDSLAGESFYSDKMNRVIELLEEKN
                     LLKESKGARIVDLEEYKMPPALITKNDGSTLYMTRDLAAAIYRKETYDFDKCIYVVGS
                     QQNLHFQQWFKVIELMGYDWAKDLIHVGFGMVALEEGTMSTRKGRVVFLEDALNQAID
                     KTKEIILAKNPNAKNVDEISKQVGVGAVVFQELSNSRIKDYTFSWERTLSFDGETGPY
                     VQYTHARCCAVLRKAEVEVTSDIDYSLLADEDSAEVLRVIESFNKNILLALKKNEPHI
                     VTRFMLDLAQAFNKFYHDNPILVENLEIRKARLALVLATKQTLENSLKLLGMHAPERM
                     "
     gene            complement(7791..8204)
                     /locus_tag="PC414_002627"
     CDS             complement(7791..8204)
                     /locus_tag="PC414_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HDF2465015.1"
                     /translation="MILVSACLIGINCKYSGDNNDNEKVKEYLKGKEFTLVCPEQLGG
                     LSTPRPPAEIVDGKVITKDNKDVTENFVRGANETLKIANLYVCSEAILKEGSPSCGCN
                     LIYDGSFSGKKIPGKGITARLLEEEGIKVKSEKDL"
     gene            complement(8272..10650)
                     /locus_tag="PC414_002628"
     CDS             complement(8272..10650)
                     /locus_tag="PC414_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HDF2465016.1"
                     /translation="MNEKSLRVLEYNKIIDLLKKKASSSLGLKYIENLVPNTDFVEVK
                     SMLEETSEAQSIIIKRGSVGLEGIHDIEDKVKRAYIGASLDPGSLIMIADTLRVARRL
                     RNSLSSSDEEDFNYPIIQSLSNSLYVYKDIEDQIYNAIISEVEISDNASSTLRDIRRR
                     IAQKNQSIRSKLNSIISSTTYQKYLQDAIISLRGDRFVVPVKSEYRSQVAGIVHDQSS
                     SGATLFIEPMTIVEMNNELRQLKLGEQEEIERILSELSAMVGEVSEDLISNQEILGRL
                     DFAFSKGKLSIQMRGIEPTLNEDKYLNIKNGRHPLLDKKKVVANTIYLGRDFHTLVIT
                     GPNTGGKTVTIKTVGLFALMTQSGLHIPADYGSSMCVYDNVFADIGDEQSIEQSLSTF
                     SSHMTNIVSILQNVTADSLVIFDELGAGTDPVEGAALAIAVLEDINSVGAKCIATTHY
                     SELKNYALTKPGVENAAVEFDIETLSPTYKLLIGVPGKSNAFEISRKLGLSDYVISRA
                     KEYINTENIALEDVLQNVEKNRIKAVEDREEAERLKEEIEKLKVEYDEKLEKLVSQRD
                     KMIEKAKSEAFSIIRQAKEEVDIIIKELRSLEQERASKEKNRKIEELRKELTSSMGSL
                     QPTVKSMIVPKVSNKEIKDLKPGEEVKVITLNQNGSVVSVDKKRKEAVVQIGIMKMTL
                     PFKSLQKTRKDVSTNVTKSTRNIIRSKSGSVKNEVDLRGLNLEEAIMEVEKYLDDAYV
                     AGLESVTVIHGIGTGVLKAGLQDILRRNRHVKSQRGGQYGEGGAGVTIVKLK"
     gene            10969..12420
                     /locus_tag="PC414_002629"
     CDS             10969..12420
                     /locus_tag="PC414_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HDF2465017.1"
                     /translation="MGNVLEGLKPESVFKNFEKISQIPRGSGNEKGISDFLLSFGKNL
                     GLETIQDESLNIIIRKPATKGYENCPGVVLQGHMDMVCEKEKNVEHDFLKDPIKLRID
                     GDMIYATGTTLGADNGIAVAMGMAILEDNTLEHPALEVLVTVNEEDGMNGADALDPSL
                     IKGQYILNMDSEEEGYLLVSCAGGKTCVVSLPVEYKEVKGDKQGLLVEVTGLLGGHSG
                     MEIVLQRANANKAIARVLSVLNVDYELASVDGGTKHNAIPREAKCVIAVNKADVESAK
                     KQINDILTAFKHEFTTSDPGMTYSVAETSVDKVLTKDCKEKVVQMSCLTPHGVQSVSL
                     DIEGLVESSTNFAIIETKESTIEFLTSVRSSVMSIRDEIADRIRLLAQALGANYDLIA
                     QYPAWEFKKGSKLEKICSETYEKLTGKVPTVMALHAGLECGLLLDKLPHAEAISIGPD
                     MFDVHTPNEHVSIPSVANVWDYVIEILKSMNQY"
     gene            complement(12624..15137)
                     /locus_tag="PC414_002630"
     CDS             complement(12624..15137)
                     /locus_tag="PC414_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDF2465018.1"
                     /translation="MEDCVVNLKKLLLLIIVITSIFTSCIFSFGINAIGNDNRTIRVA
                     YPIQKGLTEIDEQGNYFGYTYEYLLEIAQYTGWNYEFVQVPGEINESITKLMKMLENG
                     EIDIMGGMLYNEPMQKIYNYAGNSYGVANTVLQALYENTDIDNIMSSQDVKKIRIAVI
                     ENSDTRLKELDEYCKMNLMSPELVYCKTNDEQLEALKSGKADVLLNVSMNPLEGVRTV
                     AKFSSKPFYFITPKGSTNLTQKLNSAINNIEQKDPYFSTTLYEKYFTPENNQMFLSDE
                     ETDYIKETEKLKVGVLLNKPPFQNIDKDTGELRGISIDLLNHICDKTGLEFELVPVKS
                     QEDLENMTKKREIDIVAGMIYDYEYSREHNIVMSRPFLSTQYTMMINNKLNENSLDGK
                     RVALAKGINYYGQFEENIVWYDNLEECIEAVNKGYADYTYGEGYSMQYYMNQPKYKNV
                     RLVPQTYKAKSICFGIIKKDKQELLNIFNKAIISISSEKMQSIVYQNTTYRQDFSLLY
                     IMQQNPIETIFIVASLLICIIGFMAFGLHTRTSMNEKISLELKKHTQLYEMTSEYFFE
                     YDYKQDKLIISMPEKGIETYYGLDKNDKESENGNIENERDKIRKEYNKKFFDVIKSQE
                     NGTTEMYSIMLDDTFHWIRVTSKIIYNDRKIPMYVIGKICNIDSEKEEKNRLLDKAQR
                     DSLTNIYNAAYIRKLTAQNLLNLSTEKNGALLIIDIDYFKSINDTYGHLLGDKVLIDV
                     AKVLDESFAKNDIVGRLGGDEFSVYINDIINKEELINKCNDVYNHIHLIKLPNGESLS
                     ISMGVVIAKPGQEYDELYQIADNALYDSKRQGRNRFKIV"
     gene            complement(15360..16181)
                     /locus_tag="PC414_002631"
     CDS             complement(15360..16181)
                     /locus_tag="PC414_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2465019.1"
                     /translation="MIDINLEKCVGCGMCESDCLVNAIKVKDDKAKVKNILCINCGHC
                     MAICPTDAIEMQGFDKNEVIEYNRESFELEPEKLLNFIKFRRSIRQYKDIEVEEEKIK
                     NIVEAGRYTPTGGNRQPIRYILVKEKLKEVKELAIQGLYNLALDTDDNDPVRSIYKNT
                     FKKMYKRYKENGNDSLFYDAPLLMVVVGDMSLGGSAYVDGGLAASNMELMAYSQGLGI
                     CYNGFFVMASNVEPKIKELLGMSENEAVITSFILGYPDVKYKRTVNRNTAKFEVR"
     gene            complement(16699..17175)
                     /locus_tag="PC414_002632"
     CDS             complement(16699..17175)
                     /locus_tag="PC414_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HDF2465020.1"
                     /translation="MKLTTICYIEKDDKTLMLYRNKKKDDIHEGKYVGVGGKFEQGET
                     PEECVIREVKEETGLTLKSLSYKGLITFPKFKDEEDWYMFLYFSDEFEGELSEKDLND
                     CKEGNLIWVDNDKIFDLNMWEGDRLFLNWAKTGNIFSAKIVYDNGKLKDYNVSFLD"
     gene            complement(17209..17787)
                     /locus_tag="PC414_002633"
     CDS             complement(17209..17787)
                     /locus_tag="PC414_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDF2465021.1"
                     /translation="MREEALEILFKYLETDRMRKHCYAVEAVMRELAKRLEPEKEEEW
                     AVAGLLHDLDSDIVGRKPGEICEGHATTTVELLKKENFGDEEMYRAILGHHDGMGVTR
                     TSLMEKAIYAADPITGFITAIALVYPDKKLTSVKTKSVIKRMKETRFASNVNRDAMRS
                     IEDIGIPFDEFAELSLNAMKNISDVLESAENN"
     gene            complement(17891..20473)
                     /locus_tag="PC414_002634"
     CDS             complement(17891..20473)
                     /locus_tag="PC414_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDF2465022.1"
                     /translation="MKKIELLAPVGSFDSLKAAVQNGANAVYLGGKDFSARASANNFD
                     RNELIEAVKYSHIRGVQVFVTVNTLIKQYEIEDFVEYVKFLYDIDIDALIVQDIGMAR
                     LIKKLLPDFELHASTQMVAHSLEDVKYLQSVGFDRVVLARELNVDEIKWICENTTVDI
                     EVFVHGALCVCYSGQCLMSSMIGNRSGNRGRCAQPCRQKYELIDINTGEVVKSNGDYL
                     LSPRDLSTIEDLDKIIEAGVLSLKIEGRMKKPEYVATVVSGYREAINEFVDKKKISIS
                     EEIINNLYTIFNRKFTKGYILGEVGKDIMNSNRPNNQGLYIGKVLDYNRKNKRLRVML
                     ENTLKKGDGINLGGGTIGRIIKDGEITTIAHKGDLIELDFVGEAKRNQLIYKTSDSEL
                     MDRVQATFQQDKEFIKTNIRAKVSIKLGKKPELFISDFCGNKSVVEGDKIVEKAIKVA
                     LSKEKIETQIQKLGNTPYKLKEIEIDLDEGVSMPISVLNQMRRDCIELLDEERIKIKD
                     RKFKKNRLKYQPNRVNKQKKLDTVPKIRVKVKNLEQLEAVLNYNVDLIYYEDINTLSK
                     ALQMVSSISIDSSISMDLYTSSSLGIPKELMGSNTNNIYTLSNTSKIDTLNKNHSNTA
                     VTDKKLIYSAPRIVRNKEYKQFEILDNIKGINKVQIGNWGSMEYFKKKDLNIDCYLNA
                     FNSESINHFKEEGASTVCLSQELNLNEIKQTLKYTDVEIEAVIYGYAPMMVTEYCPMG
                     VVVRDCKKDKRVAKCKESSYALRDFTDASYRITQDIFCRSTIYNSKVTCMLDNLYELE
                     EIGIDVFRIDFTVEDSEMVKKVIEAHIEVLSNNYKLGKRATDLYKKLDEIGTVAGHYY
                     KGVE"
BASE COUNT         6502 a         3685 c         2294 g         8142 t
ORIGIN      
        1 atcaaatatc tatagtaaat acttaactat acttaaaaag catataaatt gatataatta
       61 tataatagat aagttaatat tgaggaggga taattatgga aataagagta ttaagatatt
      121 ttttagcaat tgcaagagaa gaaagtatat ctggtgcggc aaccttttta catataacac
      181 aaccaacact ttctagacaa attatggaac ttgaagaaga gttgggagtt aaattattgg
      241 ttcgaggaaa tagaaaaatc accttaacaa atgaaggtag gcttttacat aaaagagcag
      301 aagaaattat atcattagtt gataaggttg aattagaatt tgacaatccc aatgagatta
      361 ttagtggtga tatatatata ggaagtggtg aaactgaagc tatgcgactt atagctagaa
      421 ctgcccatca ttttcatcaa aaatacccca atgtaagata tcaccttttt agtgggaatg
      481 ctgatgatgt gactgaaaaa ttagacaagg gactgcttga ttttggtatt ttaattgaac
      541 ctgcaaatat acagaagtat gattatgcac aaattcctgc tactgatgta tggggtttac
      601 taatgcgtaa agatagtgcc cttgcatcac aaagcacaat aaatccaaaa gatttatgca
      661 atatacctct tattatttca agacagacac ttgttgataa agcaatttct aaatggcttc
      721 aagacagtaa tgaaaaacta aacattattg caacatacaa tttagtttat aacgcttctc
      781 ttttggtaga agaaggtctg ggctacgctc tgtgtttaga tagactcgtc aatactacag
      841 aaaacagtaa tctttgtttt agacctctat ctcctccttt agaagctaaa ttaaatatta
      901 tatggaaaaa acatcaaata ttttcaaagg cagcagaaaa atttttactt caaatacaaa
      961 aagatttttc ttctatcatg ttggatacat ctaagttgga tacacctaaa gaaaaataaa
     1021 tcataataaa aggtgagagt gtctcaaaat gaactttgca ctgtataagg cactcttttt
     1081 tatacaaagt aaaatatatg ttcatcattt cagcactaaa aaagaggctt atctctattt
     1141 tgagatagcc ccacttaacc aataaaatta aatacaactt aacaatatcc ttacttattt
     1201 attctaagtt ctcttagaca agcaatagta atttcatctt gtggatttaa ttcataagct
     1261 ctttctatat agtaagttgc atcatcaata tcaccattat ttaaataagc cattcctaaa
     1321 ttgcaaagta tttctgggtc ttcctttatc ttagccgctt gttcaaaata ctctattgct
     1381 ttatctaaat tattcttata agcctcacaa agccccaatt ccactatagt atccacttga
     1441 ttaggcttta ttatcaatat cttctcaaga tacatcttag cctgctctat ttcgcccata
     1501 cccttatatc caagtgctat cataaacagc aagttccacc aatccatatg ttcttcttct
     1561 aatggaagta acttctctaa tccttcctta aacttacctt ggaatacaag tgtatatcct
     1621 tcttcatatt gaactttaaa gtccatcttg cccagattct cttgtacctc agctactaaa
     1681 tcagcatcta accctagcct taacgcttct tcccaagtca gcttagtctt taaatattga
     1741 ccttgattat aataatggta gcctaagtga taataaccta atgcaaaatt ctcatcaaca
     1801 tcaattacct tttcaagctt agccatggct tcaagtaaga atgcattcat tgccttatca
     1861 tccatatctt tttgatactg tttagctatc tcttggcaaa caatcgcata gttatacatt
     1921 gcatctaaat catctggata cattgttata aatgctttta tatatattaa agaatctgta
     1981 tgttcaccaa tctcaaagta ttttctcgac atatatccca tatacgattt caaatccaaa
     2041 tcaatatctt ttgataaagc atttaaaaac ttctcatact ctgcattgtt tttaaactta
     2101 ctatctattc caattatata tatcattgca tctgctatag acattgaatt taacttatct
     2161 tgagcagtct tttcttttat cccttttact aatacctcat tttttatagg tacatctaat
     2221 ccaccttttg gcacttcata gctttttaat ttaaactctc caccatgttt tatagttata
     2281 aaagctaatt cttctgcttt ttttagcaaa tatttttcta tcttaaaatc cattttatcc
     2341 tcctaaatac ttaaatctct actttatgag ttttagattt ctcaaatatt tttttatcat
     2401 catactcaaa taagactaca ttttctactt ctcttatttc tgtatttata tcatttttta
     2461 aatccacaac attaaatcta aatggctcaa aatgaacata ttttattata tgtttagctg
     2521 gtttatcaac aaaactaggt aaatatgttg ataagttatc attatcttgt aaaaatacat
     2581 ggcatctatt cttaaaatca tccacatcta gcttattaaa aaactgtggt atatttgatg
     2641 ttttacccag tgatatatag tcatatttta atatatcgtt gaatacatca ttatctatgt
     2701 tgattttttc attatagaaa tccatcaata ttttatataa ctgattcttt ccttgtgcca
     2761 aatcaaagta gccattttca tcaaaatact tggcaaaaac ctcaaagaat ttaaaagggc
     2821 tttttttata aaatctgcct ataatatatc tcatagataa cacaaagttt ttagaattgt
     2881 agtatctctc cagtatatct tctatatcct ttagcttcaa agtttcttca tagcttatat
     2941 aattattgta taaaaactca tatggagcat aatccttata tttaaaacca tgctcatctg
     3001 caacttttct catacctgtc cctttaatca tctttaaaaa gcctaattgc aaatgctcta
     3061 tacctagatt aaatacatca ttaaaagaat tttcaaagct cttataatct tcataaggca
     3121 gtcctgcaat taagtctaag tgctgatgta tatttctata agatgctaca gtttgtacaa
     3181 catgagacaa ctttttaaaa tcatcccttc tacctactgc atcaagtgtt ttttcattcg
     3241 ttgactgaac acctatttca aattgaaaaa gaccttcttt acaatcagcc aaaaactcaa
     3301 gcatcttatc atctatcaaa tacgctgtaa cttcaaaatg gtaagttgta tatccattat
     3361 catttttcat aaggaaatcc atgatttcca tagccacttt tctatttgca ttgaatgtcc
     3421 tatctataaa ctttatctgt gatactcttg catctattaa agcttttaaa tcactcttta
     3481 tcctgtctaa actaaaatac cttagacctg gaatagtaga tgataaacag tattggcagt
     3541 taaaagggca acctcttgat gattcataat acactattct attttcatat tcttttggat
     3601 tcaagttttc ataaggacta ggaatctcat ccaaatcttg aagtaattcc ataggcttat
     3661 tcactattat ctcattgcct tgtctatata caagtccatt tacatcattt atttccatct
     3721 ttccttgcaa gtgaagtact aagtctctaa aaattaattc tccctctcca taaagtatat
     3781 aatccacaaa atcatacttt ttcattgcct cataactatc ataactcact tccggtccgc
     3841 ccaaagctat tttagtatta ggtctaattt tttttagatt atcacaaagt ttaactatat
     3901 ctcccacatt ccagatatat gttgagaata atattatatc atattcattt ttatatatat
     3961 cctttaaaat atagtccaaa tcgttattta tcgtgtattc tttcatatta acttctatca
     4021 aatctcttac aaattctttt agatatctta tagccaaatt tgtatgaata aacttggaat
     4081 ttaatgttgt taataaaatt ttcaaatatt tctcacctct ttttaagatt atactgtctt
     4141 tttgtacccc ttataggtta aaatagtata tgtaaagtct aaataatatc tatataatta
     4201 atttattaat ttcaagtaat gtgaggaaag gaagtttttt atggaattaa ttgaaaaagt
     4261 atctatcaac tctttatcaa aaagagattt actacttatt ataaagtctc ttgaatacac
     4321 taatgaaaat actcaaatta gtgatttcat tgaccttagg aataatatag taaaggaatt
     4381 atgtttttta actgacacta aggaaaaaga ttttttagat tatctagaaa aaaatagcac
     4441 actctaataa cctaaacctt tttaaacttt aatatataaa tacctgattt agaatttatt
     4501 tttatgctta atatattatc attaaaggtt ttcgacataa ctgttatttg attaaatata
     4561 gggtctatta tagtattacc ttcttcaagt cttataagac ttcttgggtc tatgctttta
     4621 ttatatgcct taactccaat tttatatttt ctcaatttaa caacatattc ggacatagaa
     4681 aaaataaaat ctatattatc ttcatttaat ttactatctt cacccaaaat ggataatata
     4741 gattttttta tgctattaat tccttcattt tctacagttc tatcttccat atatacatca
     4801 tatagaacat ttaaaacatt taataaaata ctttcttcat cataatcttc agatttcaaa
     4861 tcaatgtact tatcaattaa ttgtattgct ttaatccttg ccatctgaaa ctttacaaca
     4921 tctgatttgt ctaattctat agaaaatcct attatcttgt ctttaatact ctgtagtaat
     4981 tcgacatact ctattttact attatttata agattgtgca taatggaatt atacaccaca
     5041 gaacctaata aataatcaaa taaaagttct tcttttttaa aataaataac atactttttt
     5101 attagtttaa taacctcatc ttcacttttt tgaatattag tatttgataa aattagtggt
     5161 aataaaatat ttaaaaaatt gatatttcct attttttctc cataaaatct atagtaaata
     5221 agttcatctc tcagtctatt ttcgtctata tacacacatc tttgcgtttt atcatctatt
     5281 aaggattttg tatttattat agttaaatct ttaagagccc tacttgcact actaaaagaa
     5341 gatgtgtaga ctttttttag taattcgtca ttcataaata tatcctttct gaaaaattga
     5401 atgtaaataa attgttatta aaaaaaaaga gacagcccgt ttaagaagct gtcatatata
     5461 gggggatagt agtgtacaaa aatcattgta atctactatc tatatattat tatacgatac
     5521 tactttttta tatgtaaagc atcagttttc gacatcccta tattttacag ttatacaaat
     5581 tgcgtactaa gttcaatttt attcttttta tttcaacatt ttatcatgct acattctttc
     5641 aggagcgtgc attcccaaca atttcagaga attttctaaa gtttgttttg ttgctaaaac
     5701 taatgcaagt cttgcttttc ttatttccaa attttctact aaaattggat tgtcatgata
     5761 aaatttatta aacgcttgag ctaaatctag cataaatcta gtcactatat gtggctcatt
     5821 tttctttaga gcaagtaaaa tattcttatt gaatgattct ataactctaa gaacttctgc
     5881 actatcttca tcagctaata gactataatc tatatcagaa gtcacttcta cttctgcttt
     5941 tcttaaaact gcacagcatc tagcatgtgt atactgaaca tatggtccag tctcaccatc
     6001 aaaactaagc gttctttccc aagagaaggt atagtcttta attctactat tagataactc
     6061 ttggaatact actgccccaa cacctacttg cttagatatt tcatccacat ttttagcatt
     6121 tgggttttta gctagtataa tttccttagt tttatctata gcttgattta aagcatcttc
     6181 taaaaatact actcttcctt ttctagtcga catagtacct tcttcaagtg caaccatacc
     6241 aaatccaaca tgaattaaat cttttgccca atcatatccc ataagctcaa ttaccttaaa
     6301 ccattgttgg aaatgtaagt tttgttgaga accaactaca tatatacact tgtcaaaatc
     6361 ataagtttct tttctatata ttgcagcagc taaatctctt gtcatatata acgttgagcc
     6421 atcattctta gtaataagtg ctggtggcat tttatactct tctaaatcaa caattcttgc
     6481 tcctttagac tcttttaaaa gatttttctc ttctaatagt tcgataactc tgttcatttt
     6541 atcagaataa aaactctctc ctgctaaaga atcaaattct atatctaaaa gtttatacac
     6601 tctgttaaac tcttttaaac tctcatttct aaaccattgc cataattctt gtgcttcttc
     6661 gtcaccattt tctaatttat taaaccatgc tcttgcttca tcttccattt ctgggtgttg
     6721 ttcagcttca tcatggaatc ttacgtataa ttttagtaat tctggaattg gattagactc
     6781 tactacttct ttttctcccc actttttaaa ggccactatt aacttaccaa actgagttcc
     6841 atagtcacct aaatgattta ttcttatagt cttgtatcct tgcgaatcat atatcttata
     6901 taaagcatta cctataactg tagttcttat gtgccctata tggaaaggtt ttgctatatt
     6961 tggagaagaa tattcaacta ttacagtttt atcttttcca aaatcactat gtccgtaatt
     7021 ttctttttca tctaaaactt ttttaattac agtttctgct aattgagatt tatttacaaa
     7081 gaaatttaca tatccgccta attgtattac ttttgttatc tctccacttg gttctatact
     7141 ttccgccaat tcacttgcta tcatgtttgg agcttttcta aacactttgg ctaatttaaa
     7201 gcatggaaaa gcatagtccc ccatatcctt atttggtgga acttctataa gtgcctctat
     7261 ctcttctttc gataaatctt ctattttttc tttaaggcaa tttgatattg ccaccttaaa
     7321 atcttgcatt ctgttgctcc tctctacatt gttcctggtt tatattgtgc ttatctcttt
     7381 atgttaaatc aaatttcgtg ttttttcaag tatttcatta cagtgtaaaa cactttagta
     7441 attatgtttt ctgtatatat taaaaatact ataattgcag tttttaatca cactattatt
     7501 gattttttat aagacttttt tctattttat tgtttcttgt actatccagt tgactttata
     7561 tacttttttt aatcttttat ctattttcat acgtcaatgc taattttata tacttttctt
     7621 ttaatctttt attaattttt atacatcaat gttaacttta tataagtttt tttaatcttt
     7681 tatctgtttt catacatcaa tgtcagcttt atatactttt tttacttttt tatctatttt
     7741 tatacatcaa tattagcttt atatactttt tttaatcttt tatctatttt ctataaatct
     7801 ttttcactct taacttttat tccttcttct tctaatagcc ttgctgttat acctttccct
     7861 ggtatctttt tacctgaaaa actaccatca tatattagat tacatccaca agaggggcta
     7921 ccctctttta gtattgcttc gctacataca tataaatttg ctatttttag agtttcatta
     7981 gcccctctta caaagttttc tgttacatcc ttattatctt tagtaattac ttttccatca
     8041 actatctcag caggtggtct tggtgtagat agaccaccta actgctctgg gcatacaagt
     8101 gtaaattctt tgccttttaa atattccttg actttttcat tatcattatt atcgccacta
     8161 tatttacaat ttattcctat taaacatgca gataccaata tcataaaaat gtgctcctat
     8221 acaaattctt aatatattct catacaaatt actaatgtat tcttatacaa attattttaa
     8281 tttaacaatt gttactcctg ctccaccttc tccatattga ccacctcttt gtgattttac
     8341 atgtctattt cttcttaaaa tgtcttgtag ccctgctttt aatactcctg tacctattcc
     8401 atgtattact gttacagact ctaaacctgc cacatatgca tcatcaagat acttttctac
     8461 ttccattata gcttcttcta ggtttaaccc tctaaggtca acttcatttt ttacactacc
     8521 agattttgac cttataatgt ttctcgttga tttagttaca tttgttgaaa catctttgcg
     8581 tgttttttgt aaagacttaa aaggtagagt catcttcatt ataccaattt gcactactgc
     8641 ttcttttctc tttttatcta ctgaaactac actaccattt tgatttaaag ttattacctt
     8701 aacttcttca cctggcttta agtcttttat ctccttattt gatacctttg gaactatcat
     8761 actttttact gttggctgaa gacttcccat agagctggtc aattcttttc taagttcttc
     8821 tatcttacga ttcttttctt ttgaagctct ttcttgttca agacttctaa gttcttttat
     8881 tataatatct acttcttctt tagcttgtct tataattgaa aatgcttctg atttagcttt
     8941 ttcaatcatc ttatctcttt gactgaccaa tttttcaagc ttttcgtcat actctacttt
     9001 cagtttttca atctcttctt ttaatctttc agcttcttct ctatcttcta ctgctttgat
     9061 tctattcttt tcaacattct gtaacacatc ttctaaagct atgttttctg tatttatata
     9121 ttctttagca cgtgaaatca cataatcact tagcccaagt tttctagaaa tctcaaacgc
     9181 atttgatttt ccgggaacac ctataagtaa cttatatgtt gggcttaaag tttctatatc
     9241 aaattcaact gctgcatttt ctacacctgg tttagtaaga gcataatttt taagctcact
     9301 atagtgagtg gtagctatac atttagctcc tacagaattt atatcttcta atactgctat
     9361 tgcaagggca gctccttcaa cagggtctgt tcccgctcct aactcgtcaa atataactaa
     9421 agagtctgct gttacgtttt gtaatataga tactatattc gtcatatgag atgaaaatgt
     9481 tgatagactc tgttcaatac tctgctcatc tcctatatct gcaaatacat tatcatatac
     9541 acacatacta cttccataat cagcaggtat atgaagtcca ctttgtgtca taagtgcaaa
     9601 tagacctaca gtttttattg taacagtctt accacctgtg tttggacctg ttatcaccaa
     9661 tgtatggaag tctcttccta aataaattgt atttgctact accttctttt tatccagcag
     9721 tggatgtctt ccatttttta tgtttaaata tttatcctca tttaaagttg gttctattcc
     9781 tctcatctgt atagaaagtt ttccctttga aaatgcaaag tccaatcttc caagtatctc
     9841 ttgattagaa attaagtctt cactaacttc tccaaccata gcagaaagct ctgataaaat
     9901 tctttctatt tcttcttgct cacctagttt taactgtctc aattcattgt tcatctctac
     9961 tatagtcatt ggctctataa aaagtgtcgc accagaagat gactggtcat gcactatacc
    10021 agctacttgc gacctatatt cagattttac aggtactaca aatctatcac ctcttagaga
    10081 aattattgca tcttgtaagt acttttgata agtagttgaa cttattattg aatttaattt
    10141 tgacctaatg gattgatttt tttgggctat tcttcttctg atatctctaa gtgtacttga
    10201 agcattgtca gatatttcta cttcactaat tatagcattg tatatctggt cttctatgtc
    10261 cttgtataca tatagagaat ttgatagaga ttgtattatt ggataattaa aatcttcttc
    10321 atcactacta gataagctat ttcttaatct tctagccact cttaaggtat ctgctatcat
    10381 aatcagactt cctgggtcaa gagaagcacc tatataagct ctctttacct tatcttctat
    10441 atcatgaata ccttccaacc ctactgaacc tctctttata ataattgatt gagcttcact
    10501 agtttcttct agcatagatt tcacttctac aaaatcagta tttggtacta aattttctat
    10561 atattttaga cctaaagaag atgaggcttt cttctttaac aaatctatta ttttattgta
    10621 ttctagaact cttaacgatt tctcattcat tttctatatt caccatcctc gatattttaa
    10681 cttttttatt taattttaaa aatgtatgtt ttcaagcatt tccaataaaa actgcaagtt
    10741 ttatttcttt ttcatttcta aaattattat attttttctc ttttagatat attttacaca
    10801 tattttgtat tatactatgt atgtagctat ctatatatta tacataaaca tgctttattt
    10861 gtaatataat ctgttaaaaa atcttcttta atatttttaa aggagtttat tttagcttat
    10921 aaaaaaaaat agctacttat atagataaat taaggaggaa catgagtcat gggaaacgta
    10981 ttagaaggat taaaaccaga aagtgttttt aaaaactttg agaaaatatc tcaaattcct
    11041 agaggttctg gaaatgaaaa aggcataagt gattttttac ttagctttgg taaaaattta
    11101 ggtcttgaaa ctattcagga tgaaagctta aacataataa taagaaagcc tgctacaaaa
    11161 ggatatgaaa attgtccagg tgtagttctt caaggacata tggatatggt ttgcgaaaaa
    11221 gaaaagaatg ttgaacatga ttttcttaaa gaccctataa aattaagaat tgatggtgac
    11281 atgatatatg ctactggaac tactctagga gctgacaatg gtatagctgt tgctatgggt
    11341 atggctatat tagaagataa tactttagag catcctgcac tagaagtttt agttactgtt
    11401 aatgaagaag atggtatgaa tggtgcagat gcactagacc ctagcttaat taaaggtcaa
    11461 tatatcctaa atatggactc cgaggaggaa ggatacttat tagttagctg tgctggtggt
    11521 aaaacttgtg ttgttagttt acctgttgaa tacaaggaag ttaaaggtga caaacaagga
    11581 ttattagttg aggttactgg actattaggt ggacactctg gtatggaaat tgttttacaa
    11641 agagcaaatg ctaacaaagc aatagctaga gtattaagtg tattgaatgt tgattatgaa
    11701 ttagcttctg ttgatggtgg tactaagcac aatgctatac ctcgtgaagc taaatgtgta
    11761 atagctgtta ataaagctga tgttgaatct gctaaaaaac aaataaatga cattttaact
    11821 gcatttaaac atgagtttac tacttctgac cctggcatga cttactcagt agctgaaact
    11881 tctgtagaca aagttcttac taaagattgt aaagaaaaag ttgttcaaat gtcttgctta
    11941 actcctcatg gagttcaatc agtaagtctt gatatagaag gattagttga aagttctact
    12001 aacttcgcta taatcgaaac taaagaatct actattgaat ttttaacttc tgttagaagt
    12061 tctgttatgt ctattagaga tgaaatcgct gatagaataa gacttttggc tcaagcttta
    12121 ggtgctaatt acgaccttat agctcaatac ccagcttggg aatttaaaaa aggctcaaaa
    12181 ttagaaaaaa tatgcagtga aacttatgag aaattaactg gtaaagttcc tactgttatg
    12241 gctctacatg ctggtcttga gtgtggatta ttacttgata aacttcctca tgctgaagct
    12301 atctctatag gaccagatat gtttgatgtt catactccaa atgaacatgt tagtatccct
    12361 tctgtagcta atgtttggga ttatgttatt gaaatattaa aatctatgaa ccaatattaa
    12421 atttaagttt tgattctaag tagattttaa ttttaagtaa tctatataaa attaactctg
    12481 taaattaata ctactgttag tataatattt actttcaata ttaacttata gaaataaagc
    12541 ctaatgatag ataataacat gtatcattag gctttattgt aatttaaata ttattaagct
    12601 ttattctgtt ttaaaattaa ttcttatact attttaaatc tatttcttcc ttgtctctta
    12661 gaatcataaa gagcattatc tgctatttga tacagttcat catactcttg accaggcttt
    12721 gctataacta cccccatact aatagataaa ctttctccat ttggcaactt aatgagatga
    12781 atatgattgt atacatcatt acatttatta ataagttctt ccttatttat tatatcattg
    12841 atataaacac taaattcatc tccacccaaa cgacctacta tatcattttt tgcaaaactt
    12901 tcatcaagta cctttgcaac atcaataagt actttatctc ctaacaagtg accataagta
    12961 tcattaatcg acttaaaata atcaatatct ataatcaata aagcaccatt tttttcagtt
    13021 gataaattca acaaattttg tgcagttaat tttctaatat atgctgcatt ataaatattg
    13081 gttaaactat ccctttgagc cttatctaac aatctattct tttcctcttt ttcagaatca
    13141 atattacata ttttcccgat tacatacatt ggtatttttc tatcattata aattattttt
    13201 gaagtaactc taatccaatg aaaagtatca tcaagcataa tagaatacat ttcagttgtt
    13261 ccattttctt gtgattttat tacatcaaaa aactttttat tatattcttt ccttatttta
    13321 tctctttcat tttctatatt gccattttca ctttccttat catttttatc caaaccataa
    13381 taagtctcaa tacccttttc tggcatagaa attatcaatt tatcttgctt ataatcatac
    13441 tcaaaaaagt attcacttgt catttcatac aactgagtat gttttttgag ttctaaagat
    13501 atcttctcgt tcatacttgt acgagtatgc aaaccaaatg ccataaatcc aataatacaa
    13561 attaataaac tggctacaat aaaaatagtc tcaattggat tctgttgcat aatataaagc
    13621 aacgagaagt cttgtctata tgtagtattt tggtagacaa ttgattgcat tttttctgat
    13681 gaaatactta taatagcttt attaaatata ttcaataatt cttgtttatc ttttttaatt
    13741 ataccaaaac aaatactctt tgctttataa gtttgaggaa ctagacgcac atttttatac
    13801 ttaggttgat tcatatagta ctgcatagaa taaccttcgc cataagtgta atctgcatag
    13861 cctttattaa cagcttctat acactcttct aaattatcat accaaactat attctcttca
    13921 aactgaccat aataattaat acctttggct agagctacac gttttccatc taaactattc
    13981 tcatttaatt tattattaat catcatagta tattgagtag aaagaaaagg ccgagacata
    14041 actatattat gttcacgaga gtattcataa tcataaatca ttcctgctac aatatcaatt
    14101 tcacgctttt tagtcatgtt ttctaaatct tcttgagatt ttacaggcac aagttcaaat
    14161 tctaagcctg ttttatcaca tatatgattt aacaaatcaa ttgatatacc tcttaattca
    14221 cctgtatctt tatctatatt ttgaaatggt ggtttgttta aaagtactcc tacttttaat
    14281 ttttctgtct ctttaatgta atcagtttct tcatcagata aaaacatttg attgttctct
    14341 ggtgtaaagt atttttcata taaagtagtt gaaaaatatg ggtctttctg ttcaatatta
    14401 ttaattgctg agttaagctt ttgagttaaa tttgtactac ctttaggagt aatgaaataa
    14461 aatggttttg atgaaaattt tgctacagta cgtacacctt ctagtgggtt catactaaca
    14521 ttaagaagta catctgcttt accactcttt agtgcttcta gttgctcatc atttgtttta
    14581 caatatacca attcaggtga cataagattc attttacaat actcatctaa ctctttaagt
    14641 ctagtatcag aattttctat tacagcaata cggatttttt tcacatcttg tgaactcatt
    14701 atgttatcaa tatctgtatt ttcatataat gcttgtagta ctgtatttgc tactccataa
    14761 ctatttcctg cataattata tattttttgc attggctcat tgtataacat acctcccatt
    14821 atatcaattt caccattttc taacatcttc attaattttg taatagattc atttatctct
    14881 ccaggaactt gaacaaattc ataattccat ccagtatact gtgcaatttc tagtaaatat
    14941 tcatatgtat acccaaaata gttaccttgc tcatctattt cagttaaacc tttttgaata
    15001 ggataagcaa cacgaatggt acgattatca ttacctatag cgttgatacc aaaagaaaat
    15061 atgcaagaag tgaatataga tgtaattaca ataattaata aaagtaattt ttttaaattc
    15121 actacacaat cctccatata gttaagattt acagtataaa ttactgcata aacttgtaga
    15181 catgtaaaaa tagattagta tataaaagga tatataaatc tacttagaaa tttaaatatc
    15241 cttaatatat acaatatatt taaattataa ctcatttata tgtctattac aacaaattta
    15301 ccctgctaaa tacctactct atatcccata attttacaag ttgtagtgaa gtttaagttc
    15361 tatctcacct caaatttagc agtgtttcta tttacagttc ttttatattt cacatctgga
    15421 taacccaata taaatgaagt gataacagct tcgttctcgc tcattcctaa cagttctttt
    15481 atctttggct ctacatttga tgccataaca aaaaatccat tataacaaat tcctaatcct
    15541 tgagaatatg ccataagttc catattagat gcagccagtc ctccatctac ataagcactt
    15601 ccacctagag acatatctcc tacaactacc ataagtagtg gggcatcata aaataaagaa
    15661 tcatttccgt tctctttata tcttttgtac attttcttaa atgtattttt atagatagaa
    15721 cgtacagggt cattgtcatc agtatctaat gccaaattat aaagaccttg tattgccagt
    15781 tctttcactt cttttagctt ttcttttact aatatgtatc ttataggctg tctattacca
    15841 cctgttggtg tgtatctacc agcttccact atgtttttta ttttttcttc ttctacctct
    15901 atatctttat attgtcttat acttcttcta aatttaataa aattcaataa cttttcgggt
    15961 tctaattcaa aactttcacg attgtattct attacttcat ttttatcaaa tccttgcatt
    16021 tctattgcat ctgttgggca aattgccata cagtgaccac agttaataca gagaatattt
    16081 ttaacctttg ctttatcatc cttaactttt attgcattta caagacagtc tgattcacac
    16141 atcccacagc ctacacattt ttctaaattt atgtctatca ttccaaatta ttcctccttt
    16201 taaaaaattt actttagtat aaaacaaata gttgtctaag tcaattttta tttttttaac
    16261 aatatactag ccttaactac actttaatgg agaaaaatat acataaattg gaacttaaat
    16321 tagaacttat tcaaagtaaa agataaaaga agtagatgtc taaaagtgaa ctttttagtt
    16381 aattagatat tctttattgt atgaaatcaa gtggatattt atcatttcaa caataaaaaa
    16441 ggcttacctc tattttggat atgtccctgt caagtagaca gactaaaaaa aggctatctt
    16501 tatgcactga gatagcccct tttcccaact actattgttt atcttaatat tagtcaataa
    16561 atttaatttg ctataacctg taagttacat tttaatttga tgtaacttta aattacattt
    16621 ttaagtaata tctttaaatt atattcttaa aatatatcat taaactacat tttaattaat
    16681 ctaaaaacta tatcttaatt aatctaagaa acttacatta taatctttta gtttcccatt
    16741 atcatataca atctttgcac taaagatatt tccagtctta gcccaattta agaacagcct
    16801 gtcaccttcc cacatattta aatcgaatat tttatcatta tcaacccaaa ttaaatttcc
    16861 ttctttacaa tcatttaaat ctttttcaga taattcacct tcaaactcat ctgagaaata
    16921 caaaaacata taccaatctt cttcatcttt aaattttgga aaagttatca accctttata
    16981 tgacaatgat ttaagagtaa gaccagtctc ttcttttact tctctaatta cacattcttc
    17041 tggagtctct ccttgctcaa actttcctcc aactcctaca tacttacctt catgtatatc
    17101 atctttcttt ttatttctgt acaacatcaa agtcttgtca tctttttcta tataacaaat
    17161 agttgtaagt ttcatgtatc tcgccttgct ttctctttat attctcaact aattattttc
    17221 agcactttca agcacatcac ttatattctt cattgcatta agagaaagct ctgcaaattc
    17281 atcaaatgga atccctatat cttctatact tctcattgca tctctattta catttgaagc
    17341 aaatctcgtt tctttcattc ttttaataac agacttagtc tttacactgg taagtttttt
    17401 atctggataa actaaagcta tagcagttat aaacccagtt atagggtctg ctgcatatat
    17461 tgctttttcc attaggctag ttcttgtaac tcccattcca tcatgatgac caagtattgc
    17521 tctatacatt tcttcgtcac cgaagttttc ttttttaagc agttctactg ttgttgtagc
    17581 atgtccctca catatttctc ctggctttct tccaactata tctgaatcca aatcatgtaa
    17641 aagacctgcc actgcccact cttcttcttt ttctggctct agtcttttgg ctaattctct
    17701 cataacagct tcaacagcat aacaatgttt tctcattcta tcagtttcta aatacttaaa
    17761 taaaatttct aaagcttctt ctctcatttt caccaatccc cctgtttttt cacatatatg
    17821 atatttttat atattaatac acataaattc aagtttttat atatattcat gtatattttt
    17881 catgttatat ctattctaca cctttatagt agtgtcctgc aactgttcca atttcatcta
    17941 atttcttata caagtctgta gccctttttc caagtttata attattactc aatacctcaa
    18001 tgtgagcttc aataaccttt ttaaccatct cactatcttc aactgtaaaa tctatcctaa
    18061 atacatctat tccaatttcc tctaactcat atagattgtc caacatacaa gtaaccttcg
    18121 aattgtaaat tgtacttcta cagaatatat cttgtgttat cctataactt gcatccgtaa
    18181 agtctcttaa agcataacta ctttccttac atttagcaac tctcttgtcc tttttacaat
    18241 ccctaacaac tactcccata ggacagtatt ctgtaaccat cataggagca tatccatata
    18301 ttactgcttc aatttctaca tctgtatatt ttaaagtttg tttaatttca tttaagttta
    18361 gttcttgtga caaacaaact gtacttgctc cctcttcttt gaagtggttt atactttcac
    18421 tattgaatgc atttaagtag caatctatat ttaagtcttt tttcttaaag tattccatag
    18481 agccccagtt accaatttga accttattaa ttccttttat attatccaat atctcaaatt
    18541 gcttgtactc tttatttcta acaatccttg gtgctgaata gattaatttt ttgtcagtga
    18601 ctgctgtatt agagtggttc ttattcaaag tatctatttt acttgtattt gacaaagtat
    18661 atatgttatt tgtatttgaa cccattagtt ctttaggaat acctaaagat gaagaagtat
    18721 ataaatccat agaaatactt gaatctatag aaatacttga gaccatttgt aaagcttttg
    18781 ataatgtatt tatatcttca taatagatta aatctacatt ataatttaat actgcttcta
    18841 attgctccaa gttcttaact ttaactctaa tcttaggaac tgtatcaagt tttttctgtt
    18901 tatttactct atttggctgg tattttaatc tattcttctt aaattttcta tcttttatct
    18961 ttattctttc ttcatctaaa agctcaatac aatcccttct catctgattt aacacactta
    19021 taggcatact tacaccttca tccaggtcaa tttctatctc ttttaactta tatggtgtat
    19081 ttccaagctt ttgaatttgt gtttctattt tttctttact taaagccacc tttatagctt
    19141 tttcaactat tttgtctcct tctaccaccg atttatttcc acaaaaatca cttataaaaa
    19201 gctctggctt tttacctagt ttaatactca cttttgcacg tatattagtt tttatgaact
    19261 ctttatcttg ctgaaatgta gcttgtactc tatccatcaa ttcgctgtca gaagtcttat
    19321 atataagctg atttctcttt gcttctccaa caaaatctaa ttctatcaaa tcccctttat
    19381 gtgctattgt agttatctct ccatccttta ttattcttcc aatagtacct ccacctaagt
    19441 taattccatc acctttctta agcgtatttt ctagcattac tctaagtctt ttgttctttc
    19501 tattataatc taaaactttt ccaatatata gcccttgatt attaggtcta ttagagttca
    19561 ttatatcctt accaacttca cctaatatat atccttttgt aaactttcta ttaaatatgg
    19621 tatacaaatt atttattatt tcctcagaaa tacttatttt tttcttatct acaaattcat
    19681 taatcgcttc tctatatcct gaaaccacag tagctacata ctctggcttt ttcattctac
    19741 cttcaatttt taaagataat acacctgctt caattatttt atccaaatct tctatagtac
    19801 ttaaatctct aggactcaac aaataatctc cattactttt tactacttct cctgtattta
    19861 tatctatcaa ctcatacttt tgtctacatg gctgtgcaca cctacctcta tttcccgacc
    19921 tattgcctat catactactc ataaggcatt gacccgagta acatacacat aaagctccat
    19981 gtacaaaaac ttctatatct acagttgtat tttcacaaat ccattttatc tcatcaacat
    20041 ttaattctct tgctaaaaca actctatcaa accctacact ctgaaggtat tttacatctt
    20101 ctaaggaatg tgctaccatt tgagtgcttg catgtagttc aaagtcaggt aatagttttt
    20161 ttataagtct tgccattcct atatcctgta ctattaaagc atcaatatct atatcgtata
    20221 aaaattttac atactctaca aaatcttcta tttcatactg ttttatcaat gtatttacag
    20281 taacaaagac ttgcactccc cttatatgag agtatttaac agcctcaatc agctcatttc
    20341 tatcaaagtt atttgcagat gctctagcac taaagtcttt accacctaaa tacacagcat
    20401 ttgctccgtt ttgaactgct gcttttaatg aatcaaaaga gcctactggt gcaagtaatt
    20461 ctattttttt catatttttc tcctctattt atacttgtta ggttaatata attatatcta
    20521 taacttaaat atttgtatta aatttatata tttcttacta tattttaaat tcttcaatca
    20581 atttatatat tagttattta tatttggttc atttgagtta aca
//
LOCUS       DAMJVK010000071        20561 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.072, whole genome shotgun sequence.
ACCESSION   DAMJVK010000071 DAMJVK010000000
VERSION     DAMJVK010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20561
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.072"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            79..3027
                     /locus_tag="PC414_002635"
     CDS             79..3027
                     /locus_tag="PC414_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2465023.1"
                     /translation="MRQRIKNLIENEDKKNPLTDEAISSRLNIRREDVTFLRNELKIE
                     DSRQRRKVVLIKAIKDILKEEKTINKNDITRRLNSIGFTVSRFTVIQYLKEIQDSGDL
                     KLDKESDDKKEEIINNRENKDIAFDKLIGSSGSLSTIIKLAKAAILYPPHGLHTIIMG
                     PTGVGKSELAECMYKFAVESSRFPKNAPFIVFNAADYSENPQLLLSQLFGHVKGAFTG
                     ADENKEGLVSKADGGILFIDEIHRLPHEGQEILFQLIDKGMVRKLGETRLTHKIDVMI
                     ISATSEPIDSHLLNTFKRRIPVTIEIPELVARPLNERFDIINNFFLIEAQRMGANIHI
                     KSDVLKALMLYDCIGNVGQLRSDIQVACARGLLNQLTNKLKEVNISISDVPGYVKQGL
                     IKIRNCRGKIENYVDGDLIIDSNLQGEIEKKNEDIYTFPDEIYKLTERRHIDLLEQGL
                     DFDVINRIIGGELESCIQKYIKQVKKVSISNNLPDISPIVGKYIVELTYEVIKIASQY
                     LSSLEPNLHLCLAVHLKAAFERLKEGKVISNAHLKEIIGNYPLEYSVAEKVAQYLKER
                     YNINLPKEEIGIIAIYFKMSSRRDMSEQKKIGVLLMAHGRVASSVCEVTNKLLGINHA
                     RYLDMPFEKKPEEMIEEATTIIKDIDEGRGVIILVDMGSLSYFGDIISGKTGINIRTI
                     SRFDTLLSIEVIRRAVLPDLTIDEIVDEVKGIEPQVKEKVSKCNNTKVDFRKPIILTL
                     CITGLGGACKIKKILEENIKSITEKVDIEPIGMIEYPSLDLEIKQISNDKNVIAIVGT
                     IDPQIANIPFFSLVDINDKAKLNQINNLIDTNQIGETNKKYELSDLIHKEDIFVDLQV
                     KSKEEVLKYMSKKLREKGYVTKNFYKKVVERDNLFPTELSDEIAIPHTDGIDVIRPAI
                     SIAILKKPIVWSKSKVKIILLLAVDQKCFNPLSTLLSFIETDEFKKIKNIKDRDFIRE
                     MILDGVNENN"
     gene            3005..3469
                     /locus_tag="PC414_002636"
     CDS             3005..3469
                     /locus_tag="PC414_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2465024.1"
                     /translation="MESMKIISSNLIFKNIEVSNNEDALKFLGQRLFDEQYVKESYIQ
                     AVVAREKKYATGLPTEIYGVAIPHTDIVHVNEPGIAIGILNKPVKFIMMGTDDTEIDV
                     KVVFMLAVKEPQEQLQLLEKLMTIFQDKNILNNIVDLSEESVSDLLNSKLKN"
     gene            3582..3884
                     /locus_tag="PC414_002637"
     CDS             3582..3884
                     /locus_tag="PC414_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2465025.1"
                     /translation="MGVTNVAKKILVACGTGVCTSTIAINKLKKALQDIGKLDMVNIT
                     QCKVVEVASKAPDYDLIICTTQVSSSIKTPVINGLPFLTGVGMDKLINDVLEELEL"
     gene            3946..5310
                     /locus_tag="PC414_002638"
     CDS             3946..5310
                     /locus_tag="PC414_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425524.1"
                     /note="with GatAB forms a phosphoenolpyruvate-dependent
                     sugar phosphotransferase transporter for galactitol;
                     subunit IIC forms the translocation channel and contains
                     the substrate binding site; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDF2465026.1"
                     /translation="MEGLYMEILNFIVGLGAQVMMPIIICIFGLILGTKLGKSLRAGL
                     TVGVGFIGLNTIIALLTDNLGPATQQMVKNLGLSLSIIDVGWPAASAIAFASTVGALI
                     IPIGLVVNIVMLITNTTQTVDVDIWDYWHFAFTGALVAGATQSVMWGVFAAVANMVIV
                     LVMADLTAPGIEEYLGMPGISLPHGFTQAFVPIAIVVNKLLDLIPGINKIEINADTLQ
                     KKFGLFGEPLIMGSVIGVIIGIAAKYDIKGILQLGVTMGAVLILIPKMAALLMEGLLP
                     VSEAAQEFIEKRFKNRGKIYIGLDSAVGIGHPVTLSVALVLVPLTILIAAILPGNKVL
                     PFADLAVIPFALVLIVPITKGNVFRTLIIGIIIITSGLLIATNLAPLFTQMALNASFK
                     MPEGATMISSICDGANPLSWVFVKVMNYKVIGGVVFGVIALGMAIYNRNRIINENKKL
                     SIEE"
     gene            5345..5818
                     /locus_tag="PC414_002639"
     CDS             5345..5818
                     /locus_tag="PC414_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465027.1"
                     /translation="MKKYKYDSKRYLLSVTIPGFFMILILIYALFNNYTNFNLNIYSL
                     LIIVCTYGIFNTFISSSNPKKIIIDSKCIHFTSFMTTHKYEIKDIEKIRIREFYNKKI
                     YLKINDGKLFKGRYWIRTNMFNNSIALYSYFIELEECLYPDSLKFRNKKFENKNI"
     gene            5990..6640
                     /locus_tag="PC414_002640"
     CDS             5990..6640
                     /locus_tag="PC414_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HDF2465028.1"
                     /translation="MLLEKLRKEVLQASLDLLNYNLVTLTGGNVSGRDEQTGYIAITP
                     SGMDYRNLTPSDIVIVDVDGNIIDGKWKASVDLSDHLYIYKHREDINSIIHTHSTYSS
                     CFAILNEPIECASTTLANEVGGSVPVAKFSPPTSKKMGKCVIEAIGDKRACLLANHGV
                     IAVGPSVGHALTAAVMLEDSAKVYYLAKSIGTPVLLPDEEIQRARDVFFNVYGQDK"
     gene            6814..7746
                     /locus_tag="PC414_002641"
     CDS             6814..7746
                     /locus_tag="PC414_002641"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HDF2465029.1"
                     /translation="MYYLLNKSTNPYFNLALEEYLFLNDKYNDDIIIIWRNEESIFIG
                     KNQNPYQEVYHDVIEKGEIPILRRISGGGTVYHDLGNINMSFIQKDRQLHEIDFLEHT
                     KFMQKMLSTLGLDVSITERKDLFLNGKKISGSAQSIKRKNSLYHGTLLYDSDLNKLTK
                     YLNSNKATESNATKSVSSKVTNIKFLLEKDINYFLDYCVEYLKSNIKNIKELELDEED
                     IKSVYNIGEDKYKKIEWTYGKTPKFQVVLPFNNESKINIHVNRWKVSKFFISYDDNVI
                     NMDKLLDLNFFKEEIKESILENYPHYIDLINLIF"
     gene            8020..8733
                     /gene="ispD"
                     /locus_tag="PC414_002642"
     CDS             8020..8733
                     /gene="ispD"
                     /locus_tag="PC414_002642"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HDF2465030.1"
                     /translation="MYSVIIVAAGSGRRMNLDINKQFIKLREKEIIAHTIQVFYENIN
                     IDEIVVCIKKEEEDFFKENIINKYNFKNIKIAYGGKERQDSIYNGLKKLDKNCDIVLI
                     HDGARPFVDHRIINESIKVAKEKKAVVVGVPVSDTIKIVSDGTVQETPERNLLWAAQT
                     PQTFEYNLIIDAYEQAYKNNYYGTDDSMLVENIGQSVTMVMGSYENIKITSPEDLNIA
                     EQILNMEKRDEVNSRRRII"
     gene            8730..9215
                     /locus_tag="PC414_002643"
     CDS             8730..9215
                     /locus_tag="PC414_002643"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HDF2465031.1"
                     /translation="MRIGLGYDVHKLTEDRKLIIGGVEIPHDKGLLGHSDADVLIHAI
                     MDSILGALALGDIGKHFPDTDEEYKGADSMKLLEHVYNLITSKGYKIGNIDSTIIAQS
                     PKMAPYIESMRSNISKVLNTDIDNINIKATTEEGLGFTGAKQGIASQSICLLLLTSQN
                     N"
     misc_binding    9310..9518
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            9582..11297
                     /locus_tag="PC414_002644"
     CDS             9582..11297
                     /locus_tag="PC414_002644"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDF2465032.1"
                     /translation="MKMSKMFMPTLKEIPADAEITSHQLMVRSGMIKKMTSGVYNQLP
                     MGLRVFKKIEQIIREELNKKDCQEILCAALLPSELWKESGRWTAMGEEMFRLKDRTER
                     EYCLGPTHEEAFTDIIRQEITSYKQLPLNLYQIQVKYRDERRPRFGVMRTKTFTMKDA
                     YSFDVDDKGLDKSYQDMFDAYVSIFNRCGLENSPVQADSGAIGGSTSAEFMVKSEVGE
                     DEVVFCSGCDYAANVERAESCNLASQKEEMKELEEVHTPGAATIKELEEFLKTSPDKF
                     AKTLVYEADGKTVVVVVRGDREVNEIKVSNAIGSVIEFALATDDVVRKVTNAEVGFAG
                     PIGINADYVFIDKEIVEQRNIVVGANKTEYHIKNANYGRDFEGIVGDFRNVQEGDKCI
                     VCGKPLEIARGVEVGHIFKLGTKYSESMNANFIDKDGKSKPIVMGCYGIGVERTAAAI
                     IEQHNDEKGIIWPLSVAPYHVVIVPANMKNEEQISIAENIYNDLQAMGVEVLLDDRDE
                     RIGVKFNDSELIGIPMRITVGKNINEGKVEFKLRHKEDKEIIDIEEINEKVKAEFIRN
                     NVRLG"
     gene            11419..12864
                     /locus_tag="PC414_002645"
     CDS             11419..12864
                     /locus_tag="PC414_002645"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDF2465033.1"
                     /translation="MAKNEKQFVEEITKMEDDFPQWYTDVITKTDLVDYAPVKGFMVV
                     KPYGYALWEKMQEFMDKKFKETGHKNCYFPLLIPESLLNKEAEHVEGFAPEVAWVTHG
                     GNKKLEERLCVRPTSETIICTMYAKWLKSYRELPYLYNQWCSVVRWEKSTRPFLRTSE
                     FLWQEGHTLHETAEEAQEETIQQLEVYKALCEELLAMPVVAGQKSESEKFAGGERTYT
                     IEAMMHDGKALQSGTSHFLGQHFTKAFDITFADREGNLANPYHTSWGASTRLIGGLIM
                     THSDNRGLVLPPRVAPIQVVIVPIAAKKGNVMETVDKIYADLKAKGVAVEVDDRDNYT
                     TGWKFNEWEMKGVPVRVEIGPKDIENNQAMVFRRDTLEKDSMPLEGLADAICDLFDVI
                     HNDMFEKARKHREDNTSIVENMDEFRKALEEKPGFIKTMWCGDAECEAKIKEETGATI
                     RCLPFEQENLGHKCVYCGKEADSMVVMAKAY"
     gene            13302..14783
                     /locus_tag="PC414_002646"
     CDS             13302..14783
                     /locus_tag="PC414_002646"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HDF2465034.1"
                     /translation="MNVRVRFAPSPTGFVHIGSLRTALYNYLFAKKMGGEYILRVEDT
                     DQSRLVEGAIENMLNAMKWAGVNHSEGVILDDSGKVVQKGEYGPYIQSQRLDIYQEYI
                     KQLLDSGKAYYCFCTKERLDEVRDAQRAAGETAKYDGHCKNLSKEEVEANIKAGIPYV
                     IRLRLPENHTIKFTDLVRGDMEFNTNDLDDQVLMKTDGFPTYHFAVVVDDYLMKITHV
                     IRGEEWVSSTPKHVYLYEAFGWEAPVFVHLPNILNKEKKKLSKRQGDVAVEDFKKKGY
                     LPEGLVNYVALVGWSPEDNQELFTMEELEKAFSVERVSKSGGVFDTEKLNWVNQHYIK
                     DGDDAYLTDLAIPFLIEDGFITEEEATNKYDFLKSMISVLKEKLQYVKEVTEHASIFF
                     GDKIEVETEEGREFLRLEHIPTLIDALREKIEKTEVLNAEFVQAMLKEIQKEYKIKGK
                     NLFMGSRIILTGQMHGPDLPKVMEVLGKETCLNRIAYVKNNIL"
     gene            15004..15159
                     /locus_tag="PC414_002647"
     CDS             15004..15159
                     /locus_tag="PC414_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465035.1"
                     /translation="MYSNIDDVKKELKELCLEYVTILEKLKDEKMITEETFEKCSSQK
                     KIFLEEQ"
     gene            15304..16701
                     /locus_tag="PC414_002648"
     CDS             15304..16701
                     /locus_tag="PC414_002648"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HDF2465036.1"
                     /translation="MKVYNTLTRTKEEFVPLEEGKVKMYVCGPTVYNYIHIGNARPFI
                     IFDTLRRYLEYRGYDVTYVQNFTDVDDKIINRSHEEGISPEEVAAKYIKEYFVDCDGL
                     GIKRATVHPQVTDNIQQIIEFIKELEDKGYAYAVNGDVYFDTNKFEGYGKLSGQKQED
                     LEAGARIEVNDQKRHPMDFVLWKAKKEGEPGWDSPWGEGRPGWHIECSVMSKRYLGET
                     IDIHAGGQDLTFPHHENEIAQSEARSGKTFSKYWMHNGYININDEKMSKSKGNFFTVR
                     DISKLYDLEIVRFFMLSAHYRNPVNFSDEMLNQAKAGLERLYNTKEKLEFTLSNLVES
                     PLTEKEVELVKELDDFRQKFIDAMDDDVNTADAVSVIFELAKLINSNVDENSSLEFAK
                     KCLDEFNELTGVLNIVNKKKDTVLDKDIEELIQKRTDAKKNKEFQLADDIRQQLLDMG
                     IVLEDTRQGVKWKRI"
     gene            16686..17099
                     /locus_tag="PC414_002649"
     CDS             16686..17099
                     /locus_tag="PC414_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HDF2465037.1"
                     /translation="MEKDIKMEKTELVTMSPLVLAYLGDTVYETYIREYLIRQNTQRK
                     VNDLHKLAIKYVKAKAQATIIHEVEIELTEEESKIYKRGRNQKSNTSPKNADIIDYKH
                     ATGFEALVGYLYLNNEIERLQYIINKGIKIIERDM"
     gene            17205..17963
                     /locus_tag="PC414_002650"
     CDS             17205..17963
                     /locus_tag="PC414_002650"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HDF2465038.1"
                     /translation="MKVKLISHTPEPEKVIAMAAKLCYSPVGTDEIEKDLTDESIEKF
                     LNMLLSIGHGSILEHASFTFSIEGISRACSHQIVRHRIASFSQQSQRYVKLEQFEYII
                     PPEIEKIEKAKELFIDSMKKDQENYDKLVEILFENHYNDLIKNGKNEKTAKRQAEKKA
                     IEDARYVFPNACETKMVFTINARSLFNFFEHRCCERAQWEIRNLAVEMLREVKKVAPI
                     LFKKTGPSCVNGSCPEGTMTCGDIVQVREKFKAL"
     gene            17976..18713
                     /gene="rlmB"
                     /locus_tag="PC414_002651"
     CDS             17976..18713
                     /gene="rlmB"
                     /locus_tag="PC414_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HDF2465039.1"
                     /translation="MASIEGRNPVIEAIKSDREIDKILIANSAKEGSIKKIIGMAKDK
                     NIIIQYVDKHKLDEVSTSHSHQGVIAYASEYKYYELDELIDLAKNKDEDPFFIILDEI
                     TDPHNLGSIIRTADAVGAHGVIIPKRRSVHITPVVAKASAGAVEYMPVCKVTNIVNTI
                     KRLKEEGLWIAAADMDGETFYKQNLTGPLGVVIGSEGFGISRLVKQNCDFIVKMPMIG
                     NVTSLNASVAGGILLYEIFRQRLDKSK"
     gene            18716..19243
                     /locus_tag="PC414_002652"
     CDS             18716..19243
                     /locus_tag="PC414_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HDF2465040.1"
                     /translation="MKKNINHYLIIDGYNIINAWDNLKELAKEDLEDSREKLIDDIIE
                     FSEFMGYKTIIVFDAYNVKNSREKVEKRKHITIVYTREHQTADSYIEKFITSLSKYDD
                     VKVATNDYAEQQMILGKGATRMSARELKLELDRSKNKMKEKNIGLRKKIQRNWLEERL
                     DKETLSKLENIRRKR"
     gene            19308..19955
                     /gene="sigH"
                     /locus_tag="PC414_002653"
     CDS             19308..19955
                     /gene="sigH"
                     /locus_tag="PC414_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HDF2465041.1"
                     /translation="MLVAKEKSYELVDNCQQDEYNIVLRASEGDKIALEYIITKYRNF
                     VKAKAKSYFLIGADKEDIIQEGMIGLYKAVRDFDGSKTNSFKCFAEICITRQIITAIK
                     TATRQKHIPLNSYVSLNKPIYDEESDRTLLDIIATSIVTDPEELIISKEELKNIESKM
                     NELLSDLELEVLELYLNGKSYQFIADKLKRDVKSIDNALQRVKRKLEKHLENRND"
     gene            20029..>20561
                     /locus_tag="PC414_002654"
     CDS             20029..>20561
                     /locus_tag="PC414_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDF2465042.1"
                     /translation="MAKAKYERTKPHVNIGTIGHVDHGKTTLTAAITKTLYDRYQLGE
                     AVDFANIDKAPEERERGITISTAHVEYETPNRHYAHVDCPGHADYVKNMITGAAQMDG
                     AILVCSATDGPMPQTREHILLSRQVGVPYIVVFLNKCDMVDDEELLELVEMEVRDLLT
                     EYDFPGDDTPIVRGSAL"
BASE COUNT         8508 a         2150 c         3850 g         6053 t
ORIGIN      
        1 aaaaaaataa taagtacaaa aaaaacaata tatggcatat aagttgctat ataaataata
       61 aggagggaac aattataaat gaggcaaaga ataaaaaact taattgaaaa tgaagataag
      121 aaaaatcctt taacagatga agcgatttca agtaggttaa atattcgaag agaagatgtt
      181 acctttttga gaaatgagtt aaaaatagaa gattcaagac aaagaagaaa agttgtttta
      241 attaaagcta taaaagatat tttaaaagaa gaaaaaacta taaataaaaa tgatataaca
      301 agaagattaa acagcattgg gtttacagta tcaagattta cagtcataca atacttaaaa
      361 gagattcaag attctggtga cctaaaactt gataaagaga gtgatgataa aaaagaagaa
      421 ataatcaata atagagaaaa taaagatata gcatttgata aactcatagg tagtagtggt
      481 agcttaagta caattataaa actagccaaa gcagcaattc tttatccacc tcatggtctt
      541 catactatta ttatggggcc aactggtgtt ggaaagagtg agttagcaga gtgtatgtat
      601 aaatttgcag tagaatcatc cagattccct aagaatgcac catttatagt ttttaatgca
      661 gcagattatt cagagaatcc acagcttttg ctatcacagt tatttggaca cgttaaaggt
      721 gcttttacag gagctgatga gaacaaagaa ggattagtat caaaggcaga tggaggaata
      781 ttgtttatag atgaaataca tagattacct catgaaggtc aagaaatact atttcaactg
      841 attgataaag gtatggtcag aaagttagga gaaacaagac taacacataa aatagatgta
      901 atgattattt cagcaacttc tgagccaata gattctcatt tacttaatac atttaaaaga
      961 cgtatacctg taacaattga aataccagaa ttagttgcaa gaccattaaa tgagaggttt
     1021 gacattataa ataatttctt cttaatagaa gctcaaagaa tgggagcaaa catacatata
     1081 aagtcagatg tattaaaagc tcttatgctt tacgattgta taggtaatgt aggtcaactt
     1141 agaagtgata ttcaagtagc ttgtgctagg ggactattaa accaacttac aaacaaacta
     1201 aaagaagtaa atataagcat atcagatgta cctggatatg taaaacaagg tcttataaaa
     1261 atcagaaatt gtagaggaaa aatagaaaat tatgtagatg gggatttaat tatagactca
     1321 aatcttcaag gtgagataga aaagaaaaat gaagatatat atacttttcc agatgaaata
     1381 tacaaactta ctgaaagaag acatattgat ttattggaac aaggtttaga ttttgatgtt
     1441 ataaatagaa tcattggagg agaattagaa agttgcattc aaaaatacat aaaacaagtt
     1501 aaaaaagttt ctattagtaa caatcttcca gatataagtc caattgttgg aaaatatatc
     1561 gtagagctaa cttatgaagt aatcaaaata gcatcacaat acctaagttc actagaaccg
     1621 aatctacatt tatgtttagc agttcaccta aaagcagctt ttgagagatt aaaagaagga
     1681 aaagtaatat caaatgctca tttgaaagaa ataataggaa attatccact agaatattct
     1741 gtagcagaaa aggtagctca atatttaaag gaaagatata acataaactt accaaaagaa
     1801 gaaataggta taatagcaat atattttaaa atgagtagta gaagagatat gtctgaacaa
     1861 aaaaagatag gtgtacttct tatggctcac ggaagagtag catcttctgt atgtgaggtt
     1921 acaaataaac tattaggaat taatcacgca aggtatttag atatgccatt tgagaagaaa
     1981 ccggaagaaa tgattgaaga agctactaca ataataaaag atattgatga aggtagaggt
     2041 gtgattatat tagtagatat gggttcactt tcatattttg gagatattat ttctggaaag
     2101 actggaatta atataaggac aatatctaga tttgatacat tgctttctat agaggttata
     2161 cgaagagcag ttttaccaga tttaacaata gacgagatag ttgatgaagt taaaggtata
     2221 gagccacaag taaaagaaaa ggttagtaaa tgtaataata caaaagtaga ttttagaaaa
     2281 cctataattt taacattatg tattactgga ttagggggag cttgtaagat aaaaaaaatc
     2341 ctagaggaaa atattaaaag tataactgaa aaggttgata tagaacctat aggtatgatt
     2401 gaatatccaa gtcttgattt agagataaaa caaataagca atgataaaaa tgtaatcgcc
     2461 atagttggaa caattgaccc tcagatagca aatatccctt tttttagtct agtagacata
     2521 aatgataaag caaagttaaa tcaaataaat aacctaattg atacaaatca aattggtgaa
     2581 acaaataaaa aatatgaatt atcagattta atacataaag aagatatatt tgtggattta
     2641 caggttaaat caaaagaaga agttctaaaa tatatgagta aaaaacttag agagaaaggt
     2701 tatgtgacaa aaaatttcta taaaaaagta gtggaaagag ataacttatt tccaactgaa
     2761 ttatcagatg aaatagcaat acctcacaca gatggaatag atgtaattag gccagctata
     2821 agtatagcca tcttaaagaa gcctattgtg tggagtaaaa gtaaagtaaa aataatactt
     2881 ttattagctg tagaccaaaa gtgttttaat ccattaagca ctctactctc atttatagaa
     2941 acagatgaat ttaagaagat aaaaaacatt aaagatagag attttattag ggagatgata
     3001 ttagatggag tcaatgaaaa taattagtag caatttaatt tttaaaaata ttgaagtgtc
     3061 taacaatgaa gatgcattaa agtttttggg acaacgtttg tttgacgaac agtatgtaaa
     3121 agaaagctac atacaagctg ttgtagcaag agaaaaaaag tatgctacag ggttaccaac
     3181 agaaatatac ggagtggcaa taccacatac agatatagta catgtaaatg agccaggaat
     3241 agcaattgga atactgaata aacctgtaaa atttataatg atgggtacag acgatactga
     3301 aattgatgta aaagttgtat ttatgttagc agtgaaagag ccacaagaac aattgcaact
     3361 attagaaaaa cttatgacta tatttcaaga taagaatata ctaaataata ttgttgactt
     3421 aagtgaagaa agtgtgagtg atttattaaa ctctaaattg aaaaattaaa catgtaaagt
     3481 atattaaaaa tgtaaaatta aacatgcaaa gtatgttaaa aaatataaaa agattattaa
     3541 agatttctaa ctatagatat ctttaaaata aaataatttc aatgggggtg actaatgtgg
     3601 ctaaaaagat attagttgca tgtggaacag gagtatgtac ttcaacaatt gctataaaca
     3661 aattaaagaa agcacttcaa gacattggta aattggatat ggttaatatt acacaatgta
     3721 aagtagtaga agttgcatca aaagcacctg attatgattt aataatttgt actacacaag
     3781 tatctagtag tataaaaaca ccagtgataa atggattacc ttttttaact ggtgtcggaa
     3841 tggataaatt aatcaatgat gtattagaag agttagaatt ataattaaaa aaattaagga
     3901 acatggtctc taaaatcata agttttagag acttaaaatt aaataatgga gggtctttat
     3961 atggagatat taaatttcat tgttggtcta ggtgcacaag taatgatgcc aataataata
     4021 tgtatttttg gtttgatatt aggtacaaaa ctaggaaaat ctttaagagc tggtttaact
     4081 gtgggagttg ggtttatagg tcttaacact attatagctt tgctaacaga taatttaggt
     4141 ccagctacac aacaaatggt taagaattta gggctaagtt tatcaataat agatgtagga
     4201 tggccagcag catcagctat agcttttgca tcaacagtag gggctttaat tattccaata
     4261 ggtcttgtgg taaatatagt tatgctaatt accaatacta ctcaaactgt tgatgtagac
     4321 atatgggatt actggcattt tgcttttaca ggagctttag tggcaggggc tactcaaagt
     4381 gtaatgtggg gagtatttgc agcagtagca aatatggtaa ttgtattggt aatggcagat
     4441 ttaactgctc caggtataga ggaatattta ggtatgccag gtatatctct tcctcatgga
     4501 tttacacagg catttgttcc aattgctata gtagtaaata aattattaga cttaatacca
     4561 ggcataaata agattgagat aaatgctgat acattgcaga aaaaatttgg attatttgga
     4621 gaacctttaa taatggggtc tgtaataggt gtaataattg gtattgctgc gaaatatgat
     4681 ataaaaggaa tattacaatt aggtgtaact atgggagctg tattaatatt aattccaaag
     4741 atggcagcat tattaatgga aggattatta ccagtatcag aagctgcaca ggaatttatt
     4801 gagaagagat ttaaaaacag aggcaagatt tatataggct tagattcagc agtaggaata
     4861 ggtcatcctg ttacattatc agttgcacta gtgttggttc ctttgactat acttatagca
     4921 gctatattgc caggaaataa agttttacca tttgcagatt tagcagtaat tccatttgca
     4981 cttgtattaa tcgtaccaat tacaaaggga aatgtgttta gaacattaat aataggaata
     5041 attataatta cttctggatt attaatcgca actaacttag ctccattatt tacccaaatg
     5101 gctttaaatg ctagttttaa aatgcctgaa ggagcaacta tgatttcaag tatttgtgat
     5161 ggtgcgaatc cattaagttg ggtatttgta aaagttatga actataaagt tattggagga
     5221 gtagtttttg gagttatagc tttaggaatg gctatttaca ataggaatag aataataaat
     5281 gaaaataaaa agctatctat agaagaataa ggttaaaaca attttattaa ggagtgtata
     5341 ggttatgaaa aaatataaat atgattctaa aagatactta ctcagtgtta caataccagg
     5401 attttttatg attttaatat tgatttatgc actgtttaat aactatacaa attttaattt
     5461 gaatatatat agtttgttaa taattgtctg tacttatggt atattcaata cttttatatc
     5521 atcaagtaat cctaaaaaaa ttattattga tagcaaatgt atacatttta cctcatttat
     5581 gactacacat aagtatgaaa ttaaagatat agaaaagata agaataagag aattttataa
     5641 taaaaaaatt tatctaaaaa ttaatgatgg aaaacttttc aaaggtaggt attggataag
     5701 aacaaatatg tttaataata gtatagcatt atatagctat ttcattgagt tggaagagtg
     5761 cttatatcca gattcactaa aatttagaaa taaaaaattt gagaataaga acatataaaa
     5821 ataaaaaatt gaattgaagc ctagcttaat cttttttgat aagatataag tttgaacttt
     5881 atactaaatt tttagaattt tcaatcaaga caataacata atagctaagc aattagaaca
     5941 caactaagat gtataaggca tgtatatgat gaatgggagg atttaattaa tgttattaga
     6001 gaaattaaga aaagaagtat tacaagcatc tttagacctt ttaaactata atttagtgac
     6061 attaacagga ggaaatgtaa gtgggagaga tgaacaaact ggctatatag caatcacccc
     6121 aagtggaatg gattatagaa atcttacacc aagtgatatt gttatagtag atgtagatgg
     6181 aaatattata gatggaaaat ggaaagcatc agtggattta tcagaccact tgtatatata
     6241 taagcataga gaagacataa atagtataat tcatacacat tctacatact caagttgttt
     6301 tgctatttta aatgaaccta tagaatgtgc aagtacaact cttgcaaatg aagtaggggg
     6361 ttcagtacca gttgcaaaat tttcgccacc aacatctaaa aagatgggaa aatgtgttat
     6421 tgaagcaatt ggtgacaaaa gagcatgtct tttagcaaat catggcgtaa ttgcagttgg
     6481 tccatctgta ggacatgctc ttacagccgc tgtaatgtta gaagattcag caaaagtata
     6541 ttacttagca aaatcaattg gcacaccagt gctactgcct gatgaagaaa ttcaaagagc
     6601 gagagatgta ttttttaatg tctatggtca ggataaataa attagataaa attttaaaag
     6661 taatatgaaa atatagatat tcaatgatat taaggagatt tataatgata taataagttt
     6721 aatagttaag aagtaaaata tgaaatgtaa tagtatataa attcatttaa gactaaaaag
     6781 aataagataa aataaaaagg agtctgtatt tctatgtatt acttattaaa caaaagcaca
     6841 aatccttact ttaatttggc attagaagaa tatctatttt taaatgataa atataatgat
     6901 gatattataa tcatatggag aaatgaagaa tccattttta taggtaaaaa tcaaaatcca
     6961 tatcaggaag tatatcatga tgttattgaa aagggagaaa ttcctatttt aagaagaata
     7021 tctggaggag gaactgttta tcatgactta ggtaatatca atatgtcatt catacaaaaa
     7081 gataggcaac ttcatgagat agattttttg gagcatacta agtttatgca aaagatgctt
     7141 tcaaccttag gactagatgt atctataact gaaagaaaag atttattttt aaatgggaaa
     7201 aaaatttcag gctctgcaca gtctataaaa agaaaaaatt ctctttatca tggaactctt
     7261 ctttacgatt cagatttaaa taaattaact aaatatttaa attcaaataa agcaactgaa
     7321 tcaaatgcaa ctaaatctgt ttcaagtaaa gttacaaata ttaagttttt actcgaaaaa
     7381 gatataaatt actttttaga ttattgtgtt gaatatttaa aatctaatat aaagaatata
     7441 aaagaacttg aattggatga agaagatatt aaaagtgtat acaatatagg agaagataaa
     7501 tataaaaaga tagagtggac atatggtaaa acgccgaaat ttcaagtagt gctaccattt
     7561 aataatgaaa gtaaaataaa tatacatgtt aacagatgga aagtatcaaa attttttatt
     7621 tcttatgatg ataatgtaat taatatggac aagttgttag atttgaattt ctttaaagaa
     7681 gaaataaaag agagtatttt agaaaattat ccacattaca tagatttaat caatttaata
     7741 ttttaatata aaacctctaa tttgataaaa ttgtcttata tcaaactaga ggtttttaat
     7801 attcttgcat attaacaacc attacaaaat tgtaaaatta aaaacaacac tattgaaaaa
     7861 tcaatatata ttgtaataaa tatatgtgtt tttcattagc aaatagacat taactattta
     7921 tacaactgaa aatttaatat ataattaatc agatggttaa aataaaaata ttaaaagttt
     7981 taataaatga tggttaaaaa taaaatacga ggtgatacca tgtatagtgt tataattgtt
     8041 gcagcgggaa gcggaaggag aatgaactta gatataaaca aacagttcat aaaattaaga
     8101 gaaaaagaaa ttattgctca tacaattcaa gttttttatg aaaatataaa tatagatgaa
     8161 atagtggtat gtataaaaaa agaagaagaa gattttttta aagaaaatat aataaataag
     8221 tataacttta aaaatataaa aatagcatat ggcggaaaag aaagacagga ctcaatatat
     8281 aatggtttaa aaaaattaga taaaaattgt gatatagtac ttatccatga tggagcaaga
     8341 ccatttgtcg accatagaat aatcaatgaa tctataaagg tagctaaaga aaaaaaggct
     8401 gtggttgtag gtgttccagt aagtgatact ataaagatag tgtcagatgg aacagtacaa
     8461 gaaactccag aaagaaattt gctgtgggca gcacaaaccc ctcaaacctt tgaatataat
     8521 ctcataatag atgcttatga acaagcatat aagaacaatt attatggtac tgatgattca
     8581 atgttggtag aaaatatagg tcaaagtgtc actatggtaa tgggttctta tgaaaacata
     8641 aaaataacta gtccagaaga tttaaatata gcagaacaaa tattaaatat ggaaaaaaga
     8701 gatgaagtta acagtagaag gagaattata tgagaatagg attaggatat gatgtacaca
     8761 aattaacaga ggatagaaaa cttataatcg gtggtgtaga gataccacac gataaaggtt
     8821 tgttgggtca ttctgatgct gatgtattga tacatgcaat aatggattct attttaggtg
     8881 ctctagcatt aggagatata gggaagcatt tcccagatac agatgaagaa tataagggag
     8941 cagatagtat gaaattatta gaacatgtgt ataatctaat aacaagtaaa ggatataaaa
     9001 ttggtaatat agacagtact ataattgctc aaagccctaa gatggctcca tatattgaga
     9061 gtatgagaag taatatatca aaagttttaa acacagacat tgataatata aacataaagg
     9121 caacaacaga agaaggtctt ggttttacag gagcaaaaca gggaatagct tcacaaagta
     9181 tttgtttatt attgttgaca agtcagaata attaatatat tattagtaat aagtttaatg
     9241 gatatcaagt aataggccta tatagaatat ctataatgta tgaatttaag tgaaattaat
     9301 ataaatataa aattagctat gataaagaaa tagtaaaatg atgaaatctt cacagagaga
     9361 aaacggtggt gagagttttc aagaggaagt ctttgaacct gtcttttagc ttaaaggtat
     9421 aaaaatcttt acggtaggaa ccgttatatt cccaaaagag agctattcat atagctaact
     9481 agagtggtac cgcggaaatt aaacctttcg tctctatact tgtatagaga tgagaggttt
     9541 tttatatttt caggaagtta atattcagga ggaatctatt aatgaaaatg tcaaaaatgt
     9601 ttatgccgac attaaaagaa atcccagcag atgctgagat tacaagccat caattaatgg
     9661 ttaggtcagg catgataaaa aaaatgactt caggtgttta taaccaatta ccaatggggt
     9721 taagagtgtt caaaaaaatt gaacaaataa ttagagaaga attaaataaa aaagattgtc
     9781 aagaaatact ttgtgcagcc ttacttcctt ctgaattatg gaaagagtct ggaaggtgga
     9841 ctgctatggg tgaagaaatg tttagactaa aagacagaac agaaagagag tactgtcttg
     9901 ggccaacaca tgaagaagca ttcactgata taataagaca agaaattact tcatataaac
     9961 aattgccact gaacttgtac caaatacaag taaaatatag agacgagaga agaccaagat
    10021 ttggtgttat gagaactaag acttttacaa tgaaggatgc ctatagtttt gatgtagatg
    10081 ataaaggttt agataagtca tatcaagata tgtttgatgc atatgtaagt atatttaata
    10141 gatgtggatt ggagaatagc cctgtacagg cagattcagg agctatagga ggttctacat
    10201 cagccgaatt tatggttaag tcagaagttg gtgaagatga agttgtattt tgtagtggat
    10261 gtgattatgc agccaatgta gaaagagcag aatcttgtaa tctagcatca caaaaagaag
    10321 aaatgaaaga attagaagag gttcatacac caggagcagc tacaataaaa gagttagaag
    10381 aatttttaaa aacttctcca gataagtttg ctaaaacttt agtatatgag gcagatggaa
    10441 aaacagttgt agtcgttgta agaggagata gagaagttaa tgaaatcaaa gtatctaatg
    10501 ccataggttc tgttatagaa tttgctcttg ctacagatga tgtagtgaga aaagtaacta
    10561 atgcagaagt aggatttgca ggtccaatag gtataaatgc tgactatgta tttatagata
    10621 aagaaattgt tgaacaaaga aacatagttg ttggggctaa taaaactgag taccatataa
    10681 aaaatgctaa ctatggaaga gattttgaag gaatagttgg agactttaga aatgttcaag
    10741 aaggcgacaa gtgtatagta tgtggtaagc cacttgaaat agctagaggt gttgaagtag
    10801 gacatatatt taaactagga actaagtact cagaatctat gaatgcaaac ttcatagaca
    10861 aagatggaaa atcaaagcct atagtaatgg gatgttatgg aataggtgtt gaaagaacag
    10921 cagcagctat aattgaacaa cataatgatg agaaaggtat aatatggcct ttgtcagtag
    10981 ctccatacca tgttgtgata gttccagcaa atatgaaaaa tgaagaacaa atatcaatag
    11041 ctgaaaatat atataatgat ttgcaagcta tgggtgtaga agttttatta gatgacagag
    11101 atgaaagaat aggtgtcaaa ttcaatgatt cagaattaat aggaatacct atgaggatta
    11161 cagtaggtaa aaatattaat gaaggtaaag tagaatttaa acttagacat aaagaagata
    11221 aagagataat tgacatagaa gaaataaatg aaaaagtaaa ggcagagttt atcaggaata
    11281 atgttaggtt aggataataa cttaaataag atattgtata gataatcaaa gttgtacttt
    11341 atagacacca ttttatctat atataaggat ataaaatggt gtttttttat aaaacaaata
    11401 aatgaggagg taattaaaat ggcaaaaaat gaaaaacaat ttgttgaaga aataacaaag
    11461 atggaagatg attttccaca atggtacaca gatgtaataa ctaagactga cttagttgat
    11521 tatgccccag taaaaggttt tatggttgta aagccatatg gatatgcatt atgggaaaag
    11581 atgcaagaat ttatggataa aaaatttaaa gaaactggac ataaaaactg ttattttcca
    11641 ttattaatac cagagagttt attaaacaaa gaagcagaac acgttgaagg ttttgctcca
    11701 gaagttgcat gggtaacaca tggaggaaat aaaaaattag aagaaagatt atgtgtaaga
    11761 cctacttcag agactataat atgtacaatg tatgcgaaat ggttaaaatc atatagagaa
    11821 ttaccatatc tttataatca atggtgctca gttgttagat gggaaaaatc aactagacct
    11881 ttcttaagaa catctgagtt tttatggcaa gaaggtcata cgttacatga aacagcagaa
    11941 gaagcacaag aagaaacaat acaacaatta gaagtatata aagcgctatg tgaagaatta
    12001 cttgcaatgc cagttgtagc tggtcaaaaa agtgaaagtg agaaatttgc aggtggtgaa
    12061 agaacatata ctatagaagc tatgatgcat gatggtaaag cattacaatc aggtacaagt
    12121 cacttcctag gtcaacactt tactaaggct tttgatatta cttttgcaga tagggaagga
    12181 aatttagcta atccatacca tacatcttgg ggagcatcta caagacttat aggtggactt
    12241 ataatgactc atagtgataa cagaggttta gtattaccac caagagtagc tccaattcaa
    12301 gttgttatag ttcctatagc tgctaaaaaa ggaaatgtaa tggagactgt tgataaaata
    12361 tatgctgatt taaaagctaa gggagtagct gtagaagttg atgatagaga taactataca
    12421 actggatgga aatttaatga gtgggaaatg aaaggtgttc cagtaagagt tgaaattgga
    12481 ccaaaagata tagaaaataa tcaagctatg gtatttagaa gagatacttt agaaaaggat
    12541 tctatgcctt tagaaggatt agctgatgct atatgtgatt tatttgatgt tatacacaat
    12601 gatatgtttg aaaaagcaag aaaacataga gaagacaata catctatagt tgaaaatatg
    12661 gatgagttta gaaaagcttt agaagaaaag ccaggattca taaagactat gtggtgtgga
    12721 gatgcagaat gtgaagctaa gattaaggaa gaaacaggag caactataag atgcttacct
    12781 tttgaacaag aaaacttagg tcacaagtgt gtgtactgtg gaaaagaagc agatagtatg
    12841 gtagtaatgg caaaagctta ctaaaataca tttaaataaa aactggggta gaaagtattt
    12901 aatactttat cccagttttt tgttttttat gagtaataaa atgtatttgt aactatgtca
    12961 taagataagt catagatact tatattaaaa agaatataat agagaactat ttttaataat
    13021 tacatattaa tgaaagcaaa atattttgtt atatagattt tcaataaagc aaacaataaa
    13081 tataaatgaa tattaataag gagtgctttt aatttttaga aaataaatga cttaaaagaa
    13141 agatttatga aattaaaact aggattttct gatataataa acatgtgtat ttttaaataa
    13201 aaaggatatt attattagat ttgattaaaa ataaacttgc aaaacaatgt actagtttaa
    13261 tattatagtt atattgaata aaatatcagg aggaagtaaa aatgaatgta agagtaaggt
    13321 ttgcaccaag tccaacagga tttgtacata taggtagttt gagaactgct ttatataact
    13381 atttatttgc aaaaaaaatg ggcggagaat atatattaag agtggaagat acagaccaaa
    13441 gtagattagt agaaggtgct atagagaata tgcttaatgc tatgaaatgg gctggggtca
    13501 accatagtga aggtgttata ttagatgata gtggaaaagt agtacaaaaa ggagaatatg
    13561 gtccatatat tcaatctcaa agattagata tataccaaga atatataaag caacttttag
    13621 atagtggaaa agcttattac tgtttctgta caaaagaaag actggatgaa gtaagagatg
    13681 ctcaaagagc agcaggagaa acagctaaat atgatggtca ttgtaagaat ctttctaaag
    13741 aagaagtaga agcgaatata aaagcaggaa taccatatgt tataagatta agattgccag
    13801 agaatcatac aattaagttt acagacttag ttagaggaga tatggaattt aatactaatg
    13861 acttagatga ccaagtatta atgaaaacag atggatttcc aacttatcat tttgcagtag
    13921 ttgttgatga ctatttgatg aaaattacac atgttataag aggagaagaa tgggtatcat
    13981 ctactccaaa acatgtttat ttatatgaag cttttggatg ggaagcacct gtatttgtgc
    14041 atttaccaaa tatacttaat aaagaaaaga aaaaattaag taaaagacaa ggtgatgtag
    14101 ctgttgaaga ctttaagaaa aaaggatatt taccagaggg attagtaaac tatgtagcat
    14161 tagttggttg gtctcctgaa gataatcaag aattgtttac aatggaggaa ttagaaaaag
    14221 ccttctctgt agaaagagta tctaaaagtg gtggagtatt tgatactgaa aaattaaatt
    14281 gggttaatca gcactatata aaagatggtg atgatgctta tctaacagac ttagcaatac
    14341 catttttaat agaagatgga tttataacag aagaagaagc tactaataaa tatgatttct
    14401 taaagagtat gatatcagta cttaaagaga aattacaata tgtaaaagaa gttactgaac
    14461 atgcaagtat attttttgga gataaaatag aggtagaaac tgaagaggga agagagttct
    14521 taagattaga acatatacct actttaatag atgctttgag agaaaaaata gaaaaaacag
    14581 aggtattaaa tgctgagttt gttcaagcta tgcttaaaga aatacaaaaa gagtacaaaa
    14641 taaaagggaa aaatttattt atgggctcaa gaataatttt gacagggcaa atgcatggtc
    14701 cagatttacc aaaagtaatg gaagttttag gaaaagaaac ttgcttaaat agaatagctt
    14761 atgtaaaaaa taatatatta tagtcataaa agtccgctaa tttagaagcg gacttttttt
    14821 gtgttagtat ttggtgaaaa tatctgctta agaaaatgaa ggtatttaag gtaataaatt
    14881 taatatataa aaagcaaagc aaaaatataa ataaaatatg aataataaac taataaaata
    14941 ttaagcatag aagaagggaa tctattattt tagtttgatt taattattaa gggggtaatt
    15001 actatgtata gtaatattga tgatgttaaa aaggaattaa aggagctatg cttagaatat
    15061 gttacaattt tagaaaaatt aaaagatgaa aaaatgatta cagaagaaac ttttgaaaag
    15121 tgctcttctc aaaaaaaaat atttttagaa gaacaataga ggatggtaat agtattatta
    15181 ttatgtagtt tgaaagatag tacgaaaaat gatagtaata ctattattta ttgcaatttt
    15241 caactataat ataatatata atgttaagaa gataatttat aaaaatacag tgaggtgatg
    15301 agtgtgaaag tttataatac attgactaga acaaaagaag aatttgttcc attagaagaa
    15361 ggaaaggtaa aaatgtatgt gtgtggtcca acagtttata actatataca cataggtaat
    15421 gcaagaccat ttataatatt tgatactcta agaaggtatt tagagtatag aggatatgat
    15481 gtaacttatg tacaaaactt tacagatgtt gatgataaga ttataaatag aagtcatgag
    15541 gagggaatat ctcctgaaga agttgcggct aagtatataa aagaatattt tgtagactgt
    15601 gatgggctag gaataaaaag agctactgtt catcctcagg ttacagataa tatacaacag
    15661 attatagagt ttataaaaga gttggaagat aagggatatg catatgcagt aaatggtgat
    15721 gtatattttg atacaaataa gtttgaaggt tatggaaaac tttcaggtca aaaacaagaa
    15781 gatttagagg caggggctag aattgaggtt aatgaccaaa aaagacatcc tatggacttt
    15841 gttctttgga aggcaaaaaa agaaggtgag ccaggatggg atagcccttg gggagaaggt
    15901 agaccaggat ggcatataga atgttctgtt atgtctaaga gatatttagg tgaaacaatt
    15961 gatatacatg caggtgggca agatttaaca ttcccacatc atgaaaatga aatagctcaa
    16021 agtgaagcta gaagtggaaa aacattctca aaatactgga tgcataatgg atatataaat
    16081 ataaatgatg aaaagatgag taaatcaaaa ggtaatttct ttacagtaag agatatatca
    16141 aaattatatg atttagagat agtaagattt ttcatgttat cagcacatta tagaaatcca
    16201 gtaaacttta gtgatgagat gttaaatcaa gctaaagcag gtcttgaaag actatataat
    16261 actaaagaaa aattagaatt tacgctaagt aatttagtag aatctccttt aacagaaaaa
    16321 gaagtagagc ttgttaaaga attagatgac tttagacaaa aattcataga tgctatggat
    16381 gatgatgtta atacagcaga cgcagtaagt gtaatatttg aattagctaa gttaataaat
    16441 tctaatgtag atgaaaattc ttctttggag tttgctaaaa aatgcttaga tgaatttaat
    16501 gaacttacag gagtattaaa tattgtaaat aagaaaaagg atactgtatt agataaagac
    16561 attgaagaat tgatacaaaa aagaactgat gcaaagaaaa ataaagagtt tcaattagct
    16621 gatgatatta gacaacaatt actagatatg ggaattgtat tagaagacac aagacaaggt
    16681 gttaagtgga aaaggatata aaaatggaaa aaactgaatt agttactatg tcaccattag
    16741 ttttagcata cttaggtgat acagtgtatg agacatatat aagagaatat ttaattagac
    16801 agaatacaca aagaaaagtt aatgatttgc ataaactagc tataaagtat gtaaaagcta
    16861 aagctcaggc aactataatt catgaagttg aaattgagtt aactgaagaa gagagtaaaa
    16921 tttataaaag gggaagaaat caaaagtcaa atacttctcc taagaatgca gatattatag
    16981 actacaaaca tgcaactggg tttgaggcat tagttggata tttgtattta aataatgaaa
    17041 tagagagact tcaatatatt atcaataaag gaatcaaaat tattgaaaga gatatgtaaa
    17101 tattaaagag tgttgtctta ataataagta ataaggcaac acttttttta taactaatgt
    17161 ataataaata tatagaaatt agtaatgaga aaggataaaa agttatgaaa gtaaaattaa
    17221 tatcacatac acctgaacca gaaaaagtaa tagctatggc agctaagcta tgttattcac
    17281 cagttgggac agacgaaata gaaaaagact taacagatga aagtatagag aaatttttaa
    17341 atatgttatt aagtataggt catgggtcaa tattagaaca tgcttcattt acattttcaa
    17401 tagaaggtat ttcaagggca tgttcacatc aaattgttag acatcgtata gctagttttt
    17461 cacaacaaag tcaaagatat gttaaattag aacaatttga gtacataatt cctccagaaa
    17521 tagaaaaaat agaaaaggca aaagagttat ttatagactc catgaaaaaa gaccaagaga
    17581 attatgataa gttagttgaa atattgtttg aaaatcatta taatgaccta ataaaaaacg
    17641 gtaaaaatga gaaaacagct aaaagacaag ctgagaagaa agcaatagaa gatgctagat
    17701 acgtatttcc aaatgcatgt gaaacaaaga tggtatttac tataaatgct agaagtttgt
    17761 ttaatttctt tgaacataga tgctgtgaaa gagcacagtg ggaaataaga aatttagctg
    17821 tggagatgtt aagagaggtt aaaaaggttg ctcctatatt atttaaaaag actggtccaa
    17881 gttgtgtaaa tggaagttgt ccagaaggaa ctatgacttg tggagatata gttcaagtta
    17941 gagaaaaatt taaggccttg taggaggtga ataatttggc gagtatagaa ggaagaaatc
    18001 cggtaataga agcaataaaa agcgacagag aaatagataa aatattaatt gcaaattcag
    18061 ctaaagaagg ttcaattaag aaaataatag gaatggctaa agataagaat ataattattc
    18121 agtatgttga taaacataag ttagatgaag taagtacaag tcattcacat caaggtgtaa
    18181 tagcttatgc aagtgagtat aagtattatg aattagatga gctaatagat ttagcaaaga
    18241 ataaagatga agacccattt ttcattatat tagatgagat aacagaccca cataatttgg
    18301 gttcaataat aaggacagca gatgcagtag gagcacatgg agttatcatt ccaaaaagaa
    18361 ggtctgtaca cataactcct gtagttgcca aagcatctgc tggagcagta gaatatatgc
    18421 cagtttgcaa ggtaaccaat atagttaata caataaaaag actcaaagaa gaagggctgt
    18481 ggattgctgc agctgatatg gatggagaaa ctttttataa acaaaatctt actggtcctc
    18541 ttggtgtagt aattggaagt gaaggttttg gaatatctag attggttaaa caaaactgtg
    18601 actttatagt aaaaatgcca atgataggta atgttacatc tttaaatgca tctgtagcag
    18661 gtggaatact tttatatgag atattcagac aaagactgga taaaagtaaa taggcatgaa
    18721 aaaaaatata aatcattact taataataga tggctacaat attataaatg cgtgggacaa
    18781 tttgaaagaa ctggctaaag aggacttaga agattctcga gaaaagctta ttgatgacat
    18841 tatagaattt tctgagttta tgggttataa gactataata gtttttgatg cttataatgt
    18901 taaaaattct cgagaaaaag ttgaaaaaag aaaacacata acaatagttt atacaagaga
    18961 acaccaaaca gcagatagtt atatagagaa atttataact tctttatcaa agtatgatga
    19021 tgtaaaagta gccactaatg actatgcaga acagcaaatg attttaggaa aaggtgctac
    19081 tagaatgtct gcaagagaat taaagttaga gttagatcgt tccaagaata aaatgaagga
    19141 aaaaaatatc ggtttgagga aaaaaattca gcgaaattgg ttggaggaaa ggttagataa
    19201 agaaacattg tcgaaacttg agaacattcg tagaaagcgt tgaaattact aggttcttta
    19261 gactataata tacctataaa tttgttttat atatggggga gatttacatg ttggtagcaa
    19321 aagaaaaaag ttatgagtta gtggacaatt gtcagcagga tgagtataac attgtattaa
    19381 gagcaagtga aggggataaa atagcactag agtacattat tactaaatac agaaattttg
    19441 taaaggcaaa agcaaagtca tattttttaa ttggagcaga taaagaagat ataatacaag
    19501 aaggaatgat aggcctttat aaggcagtta gagattttga tggaagtaag accaattcat
    19561 ttaaatgttt tgcggagata tgtataacaa gacaaataat aactgctata aaaactgcaa
    19621 caagacaaaa acatatacct ttaaattcat atgtttcttt gaataaacca atatatgatg
    19681 aagagtcaga cagaacactt ttagatatta tagctacaag tatagtaaca gatccagaag
    19741 agcttataat tagtaaagaa gagttaaaaa atatagaatc aaaaatgaac gaattgttaa
    19801 gtgacttgga attagaagta ctagaattat atttaaatgg aaagtcatac cagtttatag
    19861 cggacaagct taaaagagat gtaaagtcaa tagataacgc tttacaaagg gttaagagaa
    19921 aattagaaaa acatcttgaa aacagaaatg attaatagta aaatacttac cataagctta
    19981 ttgtgagcaa aattaattta tatcgaaaga taagggagga aattcaaaat ggctaaagct
    20041 aaatacgaaa gaacaaaacc tcatgttaat atagggacaa taggacacgt agaccacggt
    20101 aaaactacat taacagcagc aataacaaaa acattatatg acagatatca attaggagaa
    20161 gcagtagatt tcgcaaacat agataaagct ccagaagaaa gagaaagagg aatcacaata
    20221 tcaacagcac acgttgagta tgaaacacca aatagacact atgcacacgt tgactgccca
    20281 ggacatgctg actacgttaa gaacatgata acaggagcag cacaaatgga cggagcaata
    20341 ttagtttgtt cagcaacaga tggaccaatg ccacaaacaa gagagcatat actattatca
    20401 agacaagttg gagtaccata tatagtagta ttcttaaaca aatgtgacat ggtagatgat
    20461 gaagagttat tagagttagt agagatggaa gtaagagatt tattaacaga atatgatttc
    20521 ccaggagatg acactccaat agtaagaggt tcagcattaa t
//
LOCUS       DAMJVK010000072        20346 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.073, whole genome shotgun sequence.
ACCESSION   DAMJVK010000072 DAMJVK010000000
VERSION     DAMJVK010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20346
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.073"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            121..675
                     /gene="tcdR"
                     /locus_tag="PC414_002655"
     CDS             121..675
                     /gene="tcdR"
                     /locus_tag="PC414_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin sigma factor TcdR"
                     /protein_id="HDF2465043.1"
                     /translation="MQKSFYELIVLARNNSVDDLQEILFMFKPLVKKLSRVLHYEEGE
                     TDLIIFFIELIKNIKLSSFSEKSDAIIVKYIHKSLLNKTFELSRRYSKMKFNFVEFDE
                     NILNMKNNYQSKSIFEEDICFFGYILKELSGIQRKVIFYKYLKGYSDREISVKLKISR
                     QAVNKAKNRAFKKIKKDYENYFNL"
     gene            993..8093
                     /gene="tcdB"
                     /locus_tag="PC414_002656"
                     /gene_synonym="toxB"
     CDS             993..8093
                     /gene="tcdB"
                     /locus_tag="PC414_002656"
                     /gene_synonym="toxB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_074049122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdB"
                     /protein_id="HDF2465044.1"
                     /translation="MSLVNRKQLEKMANVRFRTQEDEYVAILDALEEYHNMSENTVVE
                     KYLKLKDINSLTDIYIDTYKKSGRNKALKKFKEYLVTEVLELKNNNLTPVEKNLHFVW
                     IGGQINDTAINYINQWKDVNSDYNVNVFYDSNAFLINTLKKTVVESAINDTLESFREN
                     LNDPRFDYNKFFRKRMEIIYDKQKNFINYYKAQREENPELIIDDIVKTYLSNEYSKEI
                     DELNTYIEESLNKITQNSGNDVRNFEEFKNGESFNLYEQELVERWNLAAASDILRISA
                     LKEIGGMYLDVDMLPGIQPDLFESIEKPSSVTVDFWEMTKLEAIMKYKEYIPEYTSEH
                     FDMLDEEVQSSFESVLASKSDKSEIFSSLGDMEASPLEVKIAFNSKGIINQGLISVKD
                     SYCSNLIVKQIENRYKILNNSLNPAISEDNDFNTTTNTFIDSIMAEANADNGRFMMEL
                     GKYLRVGFFPDVKTTINLSGPEAYAAAYQDLLMFKEGSMNIHLIEADLRNFEISKTNI
                     SQSTEQEMASLWSFDDARAKAQFEEYKRNYFEGSLGEDDNLDFSQNIVVDKEYLLEKI
                     SSLARSSERGYIHYIVQLQGDKISYEAACNLFAKTPYDSVLFQKNIEDSEIAYYYNPG
                     DGEIQEIDKYKIPSIISDRPKIKLTFIGHGKDEFNTDIFAGFDVDSLSTEIEAAIDLA
                     KEDISPKSIEINLLGCNMFSYSINVEETYPGKLLLKVKDKISELMPSISQDSIIVSAN
                     QYEVRINSEGRRELLDHSGEWINKEESIIKDISSKEYISFNPKENKITVKSKNLPELS
                     TLLQEIRNNSNSSDIELEEKVMLTECEINVISNIDTQIVEERIEEAKNLTSDSINYIK
                     DEFKLIESISDALCDLKQQNELEDSHFISFEDISETDEGFSIRFINKETGESIFVETE
                     KTIFSEYANHITEEISKIKGTIFDTVNGKLVKKVNLDTTHEVNTLNAAFFIQSLIEYN
                     SSKESLSNLSVAMKVQVYAQLFSTGLNTITDAAKVVELVSTALDETIDLLPTLSEGLP
                     IIATIIDGVSLGAAIKELSETSDPLLRQEIEAKIGIMAVNLTTATTAIITSSLGVASG
                     FSILLVPLAGISAGIPSLVNNELVLRDKATKVVDYFKHVSLVETEGVFTLLDDKIMMP
                     QDDLVISEIDFNNNSIVLGKCEIWRMEGGSGHTVTDDIDHFFSAPSITYREPHLSIYD
                     VLEVQKEELDLSKDLMVLPNAPNRVFAWETGWTPGLRSLENDGTKLLDRIRDNYEGEF
                     YWRYFAFIADALITTLKPRYEDTNIRINLDSNTRSFIVPIITTEYIREKLSYSFYGSG
                     GTYALSLSQYNMGINIELSESDVWIIDVDNVVRDVTIESDKIKKGDLIEGILSTLSIE
                     ENKIILNSHEINFSGEVNGSNGFVSLTFSILEGINAIIEVDLLSKSYKLLISGELKIL
                     MLNSNHIQQKIDYIGFNSELQKNIPYSFVDSEGKENGFINGSTKEGLFVSELPDVVLI
                     SKVYMDDSKPSFGYYSNNLKDVKVITKDNVNILTGYYLKDDIKISLSLTLQDEKTIKL
                     NSVHLDESGVAEILKFMNRKGNTNTSDSLMSFLESMNIKSIFVNFLQSNIKFILDANF
                     IISGTTSIGQFEFICDENDNIQPYFIKFNTLETNYTLYVGNRQNMIVEPNYDLDDSGD
                     ISSTVINFSQKYLYGIDSCVNKVVISPNIYTDEINITPVYETNNTYPEVIVLDANYIN
                     EKINVNINDLSIRYVWSNDGNDFILMSTSEENKVSQVKIRFVNVFKDKTLANKLSFNF
                     SDKQDVPVSEIILSFTPSYYEDGLIGYDLGLVSLYNEKFYINNFGMMVSGLIYINDSL
                     YYFKPPVNNLITGFVTVGDDKYYFNPINGGAASIGETIIDDKNYYFNQSGVLQTGVFS
                     TEDGFKYFAPANTLDENLEGEAIDFTGKLIIDENIYYFDDNYRGAVEWKELDGEMHYF
                     SPETGKAFKGLNQIGDYKYYFNSDGVMQKGFVSINDNKHYFDDSGVMKVGYTEIDGKH
                     FYFAENGEMQIGVFNTEDGFKYFAHHNEDLGNEEGEEISYSGILNFNNKIYYFDDSFT
                     AVVGWKDLEDGSKYYFDEDTAEAYIGLSLINDGQYYFNDDGIMQVGFVTINDKVFYFS
                     DSGIIESGVQNIDDNYFYIDDNGIVQIGVFDTSDGYKYFAPANTVNDNIYGQAVEYSG
                     LVRVGEDVYYFGETYTIETGWIYDMENESDKYYFNPETKKACKGINLIDDIKYYFDEK
                     GIMRTGLISFENNNYYFNENGEMQFGYINIEDKMFYFGEDGVMQIGVFNTPDGFKYFA
                     HQNTLDENFEGESINYTGWLDLDEKRYYFTDEYIAATGSVIIDGEEYYFDPDTAQLVI
                     SE"
     gene            8215..8715
                     /gene="tcdE"
                     /locus_tag="PC414_002657"
     CDS             8215..8715
                     /gene="tcdE"
                     /locus_tag="PC414_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin-like glycosylating toxin export protein
                     TcdE"
                     /protein_id="HDF2465045.1"
                     /translation="MHSSSPFYISNGNKIFFYINLGGVMNMTISFLSEHIFIKLVILT
                     ISFDTLLGCLSAIKSRKFNSSFGIDGGIRKVAMIACIFFLSVVDILTKFNFLFMLPQD
                     CINFLRLKHLGISEFFSILFILYESVSILKNMCLCGLPVPKRLKEKIAILLDAMTDEM
                     NAKDEK"
     gene            8794..8955
                     /locus_tag="PC414_002658"
     CDS             8794..8955
                     /locus_tag="PC414_002658"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465046.1"
                     /translation="MKLIMKTREQMPRDTQVLNTYNFEASVHYYIDDKVVYQTLVHKD
                     GAWSVGKIY"
     gene            9020..9226
                     /locus_tag="PC414_002659"
     CDS             9020..9226
                     /locus_tag="PC414_002659"
                     /inference="COORDINATES: protein motif:HMM:NF022413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465047.1"
                     /translation="MKLFKNTKIRDEGQNDIENKYQTLINDLIDSLKVIGKIYKEIKT
                     LKKYKICLQITIRQSPYLIEESIN"
     gene            9443..17575
                     /gene="tcdA"
                     /locus_tag="PC414_002660"
                     /gene_synonym="toxA"
     CDS             9443..17575
                     /gene="tcdA"
                     /locus_tag="PC414_002660"
                     /gene_synonym="toxA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_105161403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdA"
                     /protein_id="HDF2465048.1"
                     /translation="MSLISKEELIKLAYSIRPRENEYKTILTNLDEYNKLTTNNNENK
                     YLQLKKLNESIDVFMNKYKTSSRNRALSNLKKDILKEVILIKNSNTSPVEKNLHFVWI
                     GGEVSDIALEYIKQWADINAEYNIKLWYDSEAFLVNTLKKAIVESSTTEALQLLEEEI
                     QNPQFDNMKFYKKRMEFIYDRQKRFINYYKSQINKPTVPTIDDIIKSHLVSEYNRDET
                     VLESYRTNSLRKINSNHGIDIRANSLFTEQELLNIYSQELLNRGNLAAASDIVRLLAL
                     KNFGGVYLDVDMLPGIHSDLFKTISRPSSIGLDRWEMIKLEAIMKYKKYINNYTSENF
                     DKLDQQLKDNFKLIIESKSEKSEIFSKLENLNVSDLEIKIAFALGSVINQALISKQGS
                     YLTNLVIEQVKNRYQFLNQHLNPAIESDNNFTDTTKIFHDSLFNSATAENSMFLTKIA
                     PYLQVGFMPEARSTISLSGPGAYASAYYDFINLQENTIEKTLKASDLIEFKFPENNLS
                     QLTEQEINSLWSFDQASAKYQFEKYVRDYTGGSLSEDNGVDFNKNTALDKNYLLNNKI
                     PSNNVEEAGSKNYVHYIIQLQGDDISYEATCNLFSKNPKNSIIIQRNMNESAKSYFLS
                     DDGESILELNKYRIPERLKNKEKVKVTFIGHGKDEFNTSEFARLSVDSLSNEISSFLD
                     TIKLDISPKNVEVNLLGCNMFSYDFNVEETYPGKLLLSIMDKITSTLPDVNKNSITIG
                     ANQYEVRINSEGRKELLAHSGKWINKEEAIMSDLSSKEYIFFDSIDNKLKAKSKNIPG
                     LASISEDIKTLLLDASVSPDTKFILNNLKLNIESSIGDYIYYEKLEPVKNIIHNSIDD
                     LIDEFNLLENVSDELYELKKLNNLDEKYLISFEDISKNNSTYSVRFINKSNGESVYVE
                     TEKEIFSKYSEHITKEISTIKNSIITDVNGNLLDNIQLDHTSQVNTLNAAFFIQSLID
                     YSSNKDVLNDLSTSVKVQLYAQLFSTGLNTIYDSIQLVNLISNAVNDTINVLPTITEG
                     IPIVSTILDGINLGAAIKELLDEHDPLLKKELEAKVGVLAINMSLSIAATVASIVGIG
                     AEVTIFLLPIAGISAGIPSLVNNELILHDKATSVVNYFNHLSESKKYGPLKTEDDKIL
                     VPIDDLVISEIDFNNNSIKLGTCNILAMEGGSGHTVTGNIDHFFSSPSISSHIPSLSI
                     YSAIGIETENLDFSKKIMMLPNAPSRVFWWETGAVPGLRSLENDGTRLLDSIRDLYPG
                     KFYWRFYAFFDYAITTLKPVYEDTNIKIKLDKDTRNFIMPTITTNEIRNKLSYSFDGA
                     GGTYSLLLSSYPISTNINLSKDDLWIFNIDNEVREISIENGTIKKGKLIKDVLSKIDI
                     NKNKLIIGNQTIDFSGDIDNKDRYIFLTCELDDKISLIIEINLVAKSYSLLLSGDKNY
                     LISNLSNTIEKINTLGLDSKNIAYNYTDESNNKYFGAISKTSQKSIIHYKKDSKNILE
                     FYNDSTLEFNSKDFIAEDINVFMKDDINTITGKYYVDNNTDKSIDFSISLVSKNQVKV
                     NGLYLNESVYSSYLDFVKNSDGHHNTSNFMNLFLDNISFWKLFGFENINFVIDKYFTL
                     VGKTNLGYVEFICDNNKNIDIYFGEWKTSSSKSTIFSGNGRNVVVEPIYNPDTGEDIS
                     TSLDFSYEPLYGIDRYINKVLIAPDLYTSLININTNYYSNEYYPEIIVLNPNTFHKKV
                     NINLDSSSFEYKWSTEGSDFILVRYLEESNKKILQKIRIKGILSNTQSFNKMSIDFKD
                     IKKLSLGYIMSNFKSFNSENELDRDHLGFKIIDNKTYYYDEDSKLVKGLININNSLFY
                     FDPIEFNLVTGWQTINGKKYYFDINTGAALTSYKIINGKHFYFNNDGVMQLGVFKGPD
                     GFEYFAPANTQNNNIEGQAIVYQSKFLTLNGKKYYFDNDSKAVTGWRIINNEKYYFNP
                     NNAIAAVGLQVIDNNKYYFNPDTAIISKGWQTVNGSRYYFDTDTAIAFNGYKTIDGKH
                     FYFDSDCVVKIGVFSTSNGFEYFAPANTYNNNIEGQAIVYQSKFLTLNGKKYYFDNNS
                     KAVTGWQTIDSKKYYFNTNTAEAATGWQTIDGKKYYFNTNTAEAATGWQTIDGKKYYF
                     NTNTAIASTGYTIINGKHFYFNTDGIMQIGVFKGPNGFEYFAPANTDANNIEGQAILY
                     QNEFLTLNGKKYYFGSDSKAVTGWRIINNKKYYFNPNNAIAAIHLCTINNDKYYFSYD
                     GILQNGYITIERNNFYFDANNESKMVTGVFKGPNGFEYFAPANTHNNNIEGQAIVYQN
                     KFLTLNGKKYYFDNDSKAVTGWQTIDGKKYYFNLNTAEAATGWQTIDGKKYYFNLNTA
                     EAATGWQTIDGKKYYFNTNTFIASTGYTSINGKHFYFNTDGIMQIGVFKGPNGFEYFA
                     PANTHNNNIEGQAILYQNKFLTLNGKKYYFGSDSKAVTGLRTIDGKKYYFNTNTAVAV
                     TGWQTINGKKYYFNTNTSIASTGYTIISGKHFYFNTDGIMQIGVFKGPDGFEYFAPAN
                     TDANNIEGQAIRYQNRFLYLHDNIYYFGNNSKAATGWVTIDGNRYYFEPNTAMGANGY
                     KTIDNKNFYFRNGLPQIGVFKGSNGFEYFAPANTDANNIEGQAIRYQNRFLHLLGKIY
                     YFGNNSKAVTGWQTINGKVYYFMPDTAMAAAGGLFEIDGVIYFFGVDGVKAPGIYG"
     gene            complement(17910..18608)
                     /gene="tcdC"
                     /locus_tag="PC414_002661"
     CDS             complement(17910..18608)
                     /gene="tcdC"
                     /locus_tag="PC414_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin anti-sigma factor TcdC"
                     /protein_id="HDF2465049.1"
                     /translation="MFSKKNEGNEFSNEGKGSSKKIIKFFKSTKGIALLAFILGVFFG
                     NISSPACSEDHEEVISNQTSVIDSQKTEIETLNSKLSDAEPWFKMKDDEKKAIEAENQ
                     RKAEEAKKAEEAKKAEEQRKKEEEEKKGYDTGITYDQLARTPDDYKYKKVKFEGKVIQ
                     VIEDGDEVQIRLAVSGNYDKVVLCSYKKSITPSRVLEDDYITIRGISAGTITYESTMG
                     GNITIPGIAVEKIN"
     gene            19034..19279
                     /locus_tag="PC414_002662"
     CDS             19034..19279
                     /locus_tag="PC414_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pathogenicity locus"
                     /protein_id="HDF2465050.1"
                     /translation="MGELQKIPGVGKATEKSLIMLGYTTIKSLKDANPAQMYEKECLM
                     RGQHIDRCQLYVYRCAVYFASTENPEPEKLKWWYWKD"
     gene            complement(19497..19766)
                     /locus_tag="PC414_002663"
     CDS             complement(19497..19766)
                     /locus_tag="PC414_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HDF2465051.1"
                     /translation="MFESRCGVCCDSCTKKEQVNCTGCPTMEKPFWGGECKVKTCCES
                     KELNHCGECDTFPCDMLLNKGKDQGFDPMVNIEQCRKWLEETVSN"
BASE COUNT         8060 a         2292 c         3080 g         6914 t
ORIGIN      
        1 atattttcat ataaaattta atttatttgc cgattatata attataatga ctgatttaat
       61 tccaatgttg tcaaaatttt caaataaatc atcattataa atataagaga ggatgatttt
      121 atgcaaaagt ctttttatga attaattgtt ttagcaagaa ataactcagt agatgatttg
      181 caagaaattt tatttatgtt taagccatta gtaaaaaaac ttagtagagt tttacattat
      241 gaagagggag aaacagattt aataatattt tttattgaat taataaaaaa tattaaatta
      301 agtagctttt cagaaaaaag cgatgctatt atagtcaaat atattcataa atcattactg
      361 aataagactt ttgagttgtc tagaagatat tctaaaatga agtttaattt tgtagaattt
      421 gatgaaaata tcttaaatat gaaaaataat tatcaaagta agtctatttt tgaggaagat
      481 atttgttttt tcggatatat tttgaaagaa ttatctggta ttcaaagaaa agttattttt
      541 tataaatatt taaaaggata ttctgataga gaaatatcag tgaaattaaa aatatctaga
      601 caagctgtta ataaggctaa aaatagagca tttaaaaaaa taaaaaaaga ctatgaaaat
      661 tattttaact tgtaattaat gagtttaaag aaatatttac aatagaaatc aaattttata
      721 attaacttta ttgtaaaatt aataacttaa tctaagaata tcttaatttt tatattttat
      781 atagaacaat gtttacatat ttatttcaga caacgtcttt attcaatcga agagcaaatt
      841 aatcaactga gtgtcttaaa tttaaaatgt taggaagtga atgtatatga aaacctaagt
      901 agatattagt atattttata aatagaaagg aggatatata aaagagtttt agcatttaga
      961 tgtaaaaata ttatagtaaa ggagaaaatt ttatgagttt agttaataga aaacagttag
     1021 aaaaaatggc aaatgtaaga tttcgtactc aagaagatga atatgttgca atattggatg
     1081 ctttagaaga atatcataat atgtcagaga atactgtagt cgaaaaatat ttaaaattaa
     1141 aagatataaa tagtttaaca gatatttata tagatacata taaaaaatct ggtagaaata
     1201 aagccttaaa aaaatttaag gaatatctag ttacagaagt attagagcta aagaataata
     1261 atttaactcc agttgagaaa aatttacatt ttgtttggat tggaggtcaa ataaatgaca
     1321 ctgctattaa ttatataaat caatggaaag atgtaaatag tgattataat gttaatgttt
     1381 tttatgatag taatgcattt ttgataaaca cattgaaaaa aactgtagta gaatcagcaa
     1441 taaatgatac acttgaatca tttagagaaa acttaaatga ccctagattt gactataata
     1501 aattcttcag aaaacgtatg gaaataattt atgataaaca gaaaaatttc ataaactact
     1561 ataaagctca aagagaagaa aatcctgaac ttataattga tgatattgta aagacatatc
     1621 tttcaaatga gtattcaaag gagatagatg aacttaatac ctatattgaa gaatccttaa
     1681 ataaaattac acagaatagt ggaaatgatg ttagaaactt tgaagaattt aaaaatggag
     1741 agtcattcaa cttatatgaa caagagttgg tagaaaggtg gaatttagct gctgcttctg
     1801 acatattaag aatatctgca ttaaaagaaa ttggtggtat gtatttagat gttgatatgt
     1861 taccaggaat acaaccagac ttatttgagt ctatagagaa acctagttca gtaacagtgg
     1921 atttttggga aatgacaaag ttagaagcta taatgaaata caaagaatat ataccagaat
     1981 atacctcaga acattttgac atgttagacg aagaagttca aagtagtttt gaatctgttc
     2041 tagcttctaa gtcagataaa tcagaaatat tctcatcact tggtgatatg gaggcatcac
     2101 cactagaagt taaaattgca tttaatagta agggtattat aaatcaaggg ctaatttctg
     2161 tgaaagactc atattgtagc aatttaatag taaaacaaat cgagaataga tataaaatat
     2221 tgaataatag tttaaatcca gctattagcg aggataatga ttttaatact acaacgaata
     2281 cctttattga tagtataatg gctgaagcta atgcagataa tggtagattt atgatggaac
     2341 taggaaagta tttaagagtt ggtttcttcc cagatgttaa aactactatt aacttaagtg
     2401 gccctgaagc atatgcggca gcttatcaag atttattaat gtttaaagaa ggcagtatga
     2461 atatccattt gatagaagct gatttaagaa actttgaaat ctctaaaact aatatttctc
     2521 aatcaactga acaagaaatg gctagcttat ggtcatttga cgatgcaaga gctaaagctc
     2581 aatttgaaga atataaaagg aattattttg aaggttctct tggtgaagat gataatcttg
     2641 atttttctca aaatatagta gttgacaagg agtatctttt agaaaaaata tcttcattag
     2701 caagaagttc agagagagga tatatacact atattgttca gttacaagga gataaaatta
     2761 gttatgaagc agcatgtaac ttatttgcaa agactcctta tgatagtgta ctatttcaga
     2821 aaaatataga agattcagaa attgcatatt attataatcc tggagatggt gaaatacaag
     2881 aaatagacaa gtataaaatt ccaagtataa tttctgatag acctaagatt aaattaacat
     2941 ttattggtca tggtaaagat gaatttaata ctgatatatt tgcaggtttt gatgtagatt
     3001 cattatccac agaaatagaa gcagcaatag atttagctaa agaggatatt tctcctaagt
     3061 caatagaaat aaatttatta ggatgtaata tgtttagcta ctctatcaac gtagaggaga
     3121 cttatcctgg aaaattatta cttaaagtta aagataaaat atcagaatta atgccatcta
     3181 taagtcaaga ctctattata gtaagtgcaa atcaatatga agttagaata aatagtgaag
     3241 gaagaagaga attattggat cattctggtg aatggataaa taaagaagaa agtattataa
     3301 aggatatttc atcaaaagaa tatatatcat ttaatcctaa agaaaataaa attacagtaa
     3361 aatctaaaaa tttacctgag ctatctacat tattacaaga aattagaaat aattctaatt
     3421 caagtgatat tgaactagaa gaaaaagtaa tgttaacaga atgtgagata aatgttattt
     3481 caaatataga tacgcaaatt gttgaggaaa ggattgaaga agctaagaat ttaacttctg
     3541 actctattaa ttatataaaa gatgaattta aactaataga atctatttct gatgcactat
     3601 gtgacttaaa acaacagaat gaattagaag attctcattt tatatctttt gaggacatat
     3661 cagagactga tgagggattt agtataagat ttattaataa agaaactgga gaatctatat
     3721 ttgtagaaac tgaaaaaaca atattctctg aatatgctaa tcatataact gaagagattt
     3781 ctaagataaa aggtactata tttgatactg taaatggtaa gttagtaaaa aaagtaaatt
     3841 tagatactac acacgaagta aatactttaa atgctgcatt ttttatacaa tcattaatag
     3901 aatataatag ttctaaagaa tctcttagta atttaagtgt agcaatgaaa gtccaagttt
     3961 acgctcaatt atttagtact ggtttaaata ctattacaga tgcagccaaa gttgttgaat
     4021 tagtatcaac tgcattagat gaaactatag acttacttcc tacattatct gaaggattac
     4081 ctataattgc aactattata gatggtgtaa gtttaggtgc agcaatcaaa gagctaagtg
     4141 aaacgagtga cccattatta agacaagaaa tagaagctaa gataggtata atggcagtaa
     4201 atttaacaac agctacaact gcaatcatta cttcatcttt gggggtagct agtggattta
     4261 gtatactttt agttccttta gcaggaattt cagcaggtat accaagctta gtaaacaatg
     4321 aacttgtact tcgagataag gcaacaaagg ttgtagatta ttttaaacat gtttcattag
     4381 ttgaaactga aggagtattt actttattag atgataaaat aatgatgcca caagatgatt
     4441 tagtgatatc agaaatagat tttaataata attcaatagt tttaggtaaa tgtgaaatct
     4501 ggagaatgga aggtggttca ggtcatactg taactgatga tatagatcac ttcttttcag
     4561 caccatcaat aacatataga gagccacact tatctatata tgacgtattg gaagtacaaa
     4621 aagaagaact tgatttgtca aaagatttaa tggtattacc taatgctcca aatagagtat
     4681 ttgcttggga aacaggatgg acaccaggtt taagaagctt agaaaatgat ggcacaaaac
     4741 tgttagaccg tataagagat aactatgaag gtgagtttta ttggagatat tttgctttta
     4801 tagctgatgc tttaataaca acattaaaac caagatatga agatactaat ataagaataa
     4861 atttagatag taatactaga agttttatag ttccaataat aactacagaa tatataagag
     4921 aaaaattatc atattctttc tatggttcag gaggaactta tgcattgtct ctttctcaat
     4981 ataatatggg tataaatata gaattaagtg aaagtgatgt ttggattata gatgttgata
     5041 atgttgtgag agatgtaact atagaatctg ataaaattaa aaaaggtgat ttaatagaag
     5101 gtattttatc tacactaagt attgaagaga ataaaattat cttaaatagc catgagatta
     5161 atttttctgg tgaggtaaat ggaagtaatg gatttgtttc tttaacattt tcaattttag
     5221 aaggaataaa tgcaattata gaagttgatt tattatctaa atcatataaa ttacttattt
     5281 ctggcgaatt aaaaatattg atgttaaatt caaatcatat tcaacagaaa atagattata
     5341 taggattcaa tagcgaatta cagaaaaata taccatatag ctttgtagat agtgaaggaa
     5401 aagagaatgg ttttattaat ggttcaacaa aagaaggttt atttgtatct gaattacctg
     5461 atgtagttct tataagtaag gtttatatgg atgatagtaa gccttcattt ggatattata
     5521 gtaataattt gaaagatgtc aaagttataa ctaaagataa tgttaatata ttaacaggtt
     5581 attatcttaa ggatgatata aaaatctctc tttctttgac tctacaagat gaaaaaacta
     5641 taaagttaaa tagtgtgcat ttagatgaaa gtggagtagc tgagattttg aagttcatga
     5701 atagaaaagg taatacaaat acttcagatt ctttaatgag ctttttagaa agtatgaata
     5761 taaaaagtat tttcgttaat ttcttacaat ctaatattaa gtttatatta gatgctaatt
     5821 ttataataag tggtactact tctattggcc aatttgagtt tatttgtgat gaaaatgata
     5881 atatacaacc atatttcatt aagtttaata cactagaaac taattatact ttatatgtag
     5941 gaaatagaca aaatatgata gtggaaccaa attatgattt agatgattct ggagatatat
     6001 cttcaactgt tatcaatttc tctcaaaagt atctttatgg aatagacagt tgtgttaata
     6061 aagttgtaat ttcaccaaat atttatacag atgaaataaa tataacgcct gtatatgaaa
     6121 caaataatac ttatccagaa gttattgtat tagatgcaaa ttatataaat gaaaaaataa
     6181 atgttaatat caatgatcta tctatacgat atgtatggag taatgatggt aatgatttta
     6241 ttcttatgtc aactagtgaa gaaaataagg tgtcacaagt taaaataaga ttcgttaatg
     6301 tttttaaaga taagactttg gcaaataagc tatcttttaa ctttagtgat aaacaagatg
     6361 tacctgtaag tgaaataatc ttatcattta caccttcata ttatgaggat ggattgattg
     6421 gctatgattt gggtctagtt tctttatata atgagaaatt ttatattaat aactttggaa
     6481 tgatggtatc tggattaata tatattaatg attcattata ttattttaaa ccaccagtaa
     6541 ataatttgat aactggattt gtgactgtag gcgatgataa atactacttt aatccaatta
     6601 atggtggagc tgcttcaatt ggagagacaa taattgatga caaaaattat tatttcaacc
     6661 aaagtggagt gttacaaaca ggtgtattta gtacagaaga tggatttaaa tattttgccc
     6721 cagctaatac acttgatgaa aacctagaag gagaagcaat tgattttact ggaaaattaa
     6781 ttattgacga aaatatttat tattttgatg ataattatag aggagctgta gaatggaaag
     6841 aattagatgg tgaaatgcac tattttagcc cagaaacagg taaagctttt aaaggtctaa
     6901 atcaaatagg tgattataaa tactatttca attctgatgg agttatgcaa aaaggatttg
     6961 ttagtataaa tgataataaa cactattttg atgattctgg tgttatgaaa gtaggttaca
     7021 ctgaaataga tggcaagcat ttctactttg ctgaaaacgg agaaatgcaa ataggagtat
     7081 ttaatacaga agatggattt aaatattttg ctcatcataa tgaagattta ggaaatgaag
     7141 aaggtgaaga aatctcatat tctggtatat taaatttcaa taataaaatt tactattttg
     7201 atgattcatt tacagctgta gttggatgga aagatttaga ggatggttca aagtattatt
     7261 ttgatgaaga tacagcagaa gcatatatag gtttgtcatt aataaatgat ggtcaatatt
     7321 attttaatga tgatggaatt atgcaagttg gatttgtcac tataaatgat aaagtcttct
     7381 acttctctga ctctggaatt atagaatctg gagtacaaaa catagatgac aattatttct
     7441 atatagatga taatggtata gttcaaattg gtgtatttga tacttcagat ggatataaat
     7501 attttgcacc tgctaatact gtaaatgata atatttacgg acaagcagtt gaatatagtg
     7561 gtttagttag agttggggaa gatgtatatt attttggaga aacatataca attgagactg
     7621 gatggatata tgatatggaa aatgaaagtg ataaatatta tttcaatcca gaaactaaaa
     7681 aagcatgcaa aggtattaat ttaattgatg atataaaata ttattttgat gagaagggca
     7741 taatgagaac gggtcttata tcatttgaaa ataataatta ttactttaat gagaatggtg
     7801 aaatgcaatt tggttatata aatatagaag ataagatgtt ctattttggt gaagatggtg
     7861 tcatgcagat tggagtattt aatacaccag atggatttaa atactttgca catcaaaata
     7921 ctttggatga gaattttgag ggagaatcaa taaactatac tggttggtta gatttagatg
     7981 aaaagagata ttattttaca gatgaatata ttgcagcaac tggttcagtt attattgatg
     8041 gtgaggagta ttattttgat cctgatacag ctcaattagt gattagtgaa tagataaaaa
     8101 tatgttaaat atatcctctt atacttaaat atataaaaat aaacaaaatg atacactaca
     8161 taaagtgttc tatctaatat gaagatttac caataaaaag gtggactatg atgaatgcac
     8221 agtagttcac ctttttatat ttctaatggt aacaaaatat ttttttatat aaacctagga
     8281 ggcgttatga atatgacaat atctttttta tcagagcata tatttataaa gttagtaatt
     8341 ttaactatat catttgatac attattagga tgtttaagtg caataaaaag tcgtaaattt
     8401 aattctagtt ttggaataga tggaggaatc agaaaagtag caatgatagc atgtatattt
     8461 tttttatcag tagttgacat tcttacaaag tttaactttt tatttatgtt accacaagat
     8521 tgtatcaatt ttttaagact aaaacatctt ggaatatctg aatttttctc tattttattt
     8581 attttatatg aaagtgtaag tatattaaaa aatatgtgct tatgtggatt accagtacct
     8641 aagagattaa aggaaaaaat agcaatttta ctagatgcaa tgacagatga aatgaatgct
     8701 aaggatgaaa agtaagtaat ggtagatata ataaagatat taacaaataa aaagtgttat
     8761 ccaaataaga atagctgaaa gttatcataa ttcatgaaac taataatgaa aacgagggag
     8821 cagatgccaa gagacacaca agtattaaat acatataatt tcgaagcaag tgttcattac
     8881 tatatagatg acaaggtagt atatcaaaca ttggttcaca aagatggtgc atggtcagtt
     8941 ggtaaaatct attaagctac attagttaca gatatcacaa actataatag ttaaacatag
     9001 aaatatgtgt aaattgtgat tgaaattatt caaaaacaca aaaatacgtg atgaaggaca
     9061 aaatgatata gaaaataagt atcaaacctt aataaatgat ttaattgata gtttaaaagt
     9121 tataggaaaa atatataaag aaataaaaac attaaaaaaa tataagatat gtttacaaat
     9181 tactatcaga caatctcctt atctaataga agagtcaatt aactaattga gtatctttaa
     9241 attgaaatgt taggaagtga tttaaatatg aaaacttaaa ttataaaaaa tcaatattaa
     9301 tttattttta aaaaatagaa aggagtgtat aagatttatt ttcaaagttt aaaaacaaga
     9361 aaatcaattt aaatttcaga aggaataaat gtggttatag aagtggattt attatcaaaa
     9421 ataataatac taggaggttt ttatgtcttt aatatctaaa gaagagttaa taaaactcgc
     9481 atatagcatt agaccaagag aaaatgagta taaaactata ctaactaatt tagacgaata
     9541 taataagtta actacaaaca ataatgaaaa taaatattta caattaaaaa aactaaatga
     9601 atcaattgat gtttttatga ataaatataa aacttcaagc agaaatagag cactctctaa
     9661 tctaaaaaaa gatatattaa aagaagtaat tcttattaaa aattccaata caagccctgt
     9721 agaaaaaaat ttacattttg tatggatagg tggagaagtc agtgatattg ctcttgaata
     9781 cataaaacaa tgggctgata ttaatgcaga atataatatt aaactgtggt atgatagtga
     9841 agcattctta gtaaatacac taaaaaaggc tatagttgaa tcttctacca ctgaagcatt
     9901 acagctacta gaggaagaga ttcaaaatcc tcaatttgat aatatgaaat tttacaaaaa
     9961 aaggatggaa tttatatatg atagacaaaa aaggtttata aattattata aatctcaaat
    10021 caataaacct acagtaccta caatagatga tattataaag tctcatctag tatctgaata
    10081 taatagagat gaaactgtat tagaatcata tagaacaaat tctttgagaa aaataaatag
    10141 taatcatggg atagatatca gggctaatag tttgtttaca gaacaagagt tattaaatat
    10201 ttatagtcag gagttgttaa atcgtggaaa tttagctgca gcatctgaca tagtaagatt
    10261 attagcccta aaaaattttg gcggagtata tttagatgtt gatatgcttc caggtattca
    10321 ctctgattta tttaaaacaa tatctagacc tagctctatt ggactagacc gttgggaaat
    10381 gataaaatta gaggctatta tgaagtataa aaaatatata aataattata catcagaaaa
    10441 ctttgataaa cttgatcaac aattaaaaga taattttaaa ctcattatag aaagtaaaag
    10501 tgaaaaatct gagatatttt ctaaattaga aaatttaaat gtatctgatc ttgaaattaa
    10561 aatagctttc gctttaggca gtgttataaa tcaagccttg atatcaaaac aaggttcata
    10621 tcttactaac ctagtaatag aacaagtaaa aaatagatat caatttttaa accaacacct
    10681 taacccagcc atagagtctg ataataactt cacagatact actaaaattt ttcatgattc
    10741 attatttaat tcagctaccg cagaaaactc tatgttttta acaaaaatag caccatactt
    10801 acaagtaggt tttatgccag aagctcgctc cacaataagt ttaagtggtc caggagctta
    10861 tgcgtcagct tactatgatt tcataaattt acaagaaaat actatagaaa aaactttaaa
    10921 agcatcagat ttaatagaat ttaaattccc agaaaataat ctatctcaat tgacagaaca
    10981 agaaataaat agtctatgga gctttgatca agcaagtgca aaatatcaat ttgagaaata
    11041 tgtaagagat tatactggtg gatctctttc tgaagacaat ggggtagact ttaataaaaa
    11101 tactgccctc gacaaaaact atttattaaa taataaaatt ccatcaaaca atgtagaaga
    11161 agctggaagt aaaaattatg ttcattatat catacagtta caaggagatg atataagtta
    11221 tgaagcaaca tgcaatttat tttctaaaaa tcctaaaaat agtattatta tacaacgaaa
    11281 tatgaatgaa agtgcaaaaa gctacttttt aagtgatgat ggagaatcta ttttagaatt
    11341 aaataaatat aggatacctg aaagattaaa aaataaggaa aaagtaaaag taacctttat
    11401 tggacatggt aaagatgaat tcaacacaag cgaatttgct agattaagtg tagattcact
    11461 ttccaatgag ataagttcat ttttagatac cataaaatta gatatatcac ctaaaaatgt
    11521 agaagtaaac ttacttggat gtaatatgtt tagttatgat tttaatgttg aagaaactta
    11581 tcctgggaag ttgctattaa gtattatgga caaaattact tccactttac ctgatgtaaa
    11641 taaaaattct attactatag gagcaaatca atatgaagta agaattaata gtgagggaag
    11701 aaaagaactt ctggctcact caggtaaatg gataaataaa gaagaagcta ttatgagcga
    11761 tttatctagt aaagaataca ttttttttga ttctatagat aataagctaa aagcaaagtc
    11821 caagaatatt ccaggattag catcaatatc agaagatata aaaacattat tacttgatgc
    11881 aagtgttagt cctgatacaa aatttatttt aaataatctt aagcttaata ttgaatcttc
    11941 tattggtgat tacatttatt atgaaaaatt agagcctgtt aaaaatataa ttcacaattc
    12001 tatagatgat ttaatagatg agttcaatct acttgaaaat gtatctgatg aattatatga
    12061 attaaaaaaa ttaaataatc tagatgagaa gtatttaata tcttttgaag atatctcaaa
    12121 aaataattca acttactctg taagatttat taacaaaagt aatggtgagt cagtttatgt
    12181 agaaacagaa aaagaaattt tttcaaaata tagcgaacat attacaaaag aaataagtac
    12241 tataaagaat agtataatta cagatgttaa tggtaattta ttggataata tacagttaga
    12301 tcatacttct caagttaata cattaaacgc agcattcttt attcaatcat taatagatta
    12361 tagtagcaat aaagatgtac tgaatgattt aagtacctca gttaaggttc aactttatgc
    12421 tcaactattt agtacaggtt taaatactat atatgactct atccaattag taaatttaat
    12481 atcaaatgca gtaaatgata ctataaatgt actacctaca ataacagagg ggatacctat
    12541 tgtatctact atattagacg gaataaactt aggtgcagca attaaggaat tactagacga
    12601 acatgaccca ttactaaaaa aagaattaga agctaaggtg ggtgttttag caataaatat
    12661 gtcattatct atagctgcaa ctgtagcttc aattgttgga ataggtgctg aagttactat
    12721 tttcttatta cctatagctg gtatatctgc aggaatacct tcattagtta ataatgaatt
    12781 aatattgcat gataaggcaa cttcagtggt aaactatttt aatcatttgt ctgaatctaa
    12841 aaaatatggc cctcttaaaa cagaagatga taaaatttta gttcctattg atgatttagt
    12901 aatatcagaa atagatttta ataataattc gataaaacta ggaacatgta atatattagc
    12961 aatggagggg ggatcaggac acacagtgac tggtaatata gatcactttt tctcatctcc
    13021 atctataagt tctcatattc cttcattatc aatttattct gcaataggta tagaaacaga
    13081 aaatctagat ttttcaaaaa aaataatgat gttacctaat gctccttcaa gagtgttttg
    13141 gtgggaaact ggagcagttc caggtttaag atcattggaa aatgacggaa ctagattact
    13201 tgattcaata agagatttat acccaggtaa attttactgg agattctatg cttttttcga
    13261 ttatgcaata actacattaa aaccagttta tgaagacact aatattaaaa ttaaactaga
    13321 taaagatact agaaacttca taatgccaac tataactact aacgaaatta gaaacaaatt
    13381 atcttattca tttgatggag caggaggaac ttactcttta ttattatctt catatccaat
    13441 atcaacgaat ataaatttat ctaaagatga tttatggata tttaatattg ataatgaagt
    13501 aagagaaata tctatagaaa atggtactat taaaaaagga aagttaataa aagatgtttt
    13561 aagtaaaatt gatataaata aaaataaact tattataggc aatcaaacaa tagatttttc
    13621 aggcgatata gataataaag atagatatat attcttgact tgtgagttag atgataaaat
    13681 tagtttaata atagaaataa atcttgttgc aaaatcttat agtttgttat tgtctgggga
    13741 taaaaattat ttgatatcca atttatctaa tactattgag aaaatcaata ctttaggcct
    13801 agatagtaaa aatatagcgt acaattacac tgatgaatct aataataaat attttggagc
    13861 tatatctaaa acaagtcaaa aaagcataat acattataaa aaagacagta aaaatatatt
    13921 agaattttat aatgacagta cattagaatt taacagtaaa gattttattg ctgaagatat
    13981 aaatgtattt atgaaagatg atattaatac tataacagga aaatactatg ttgataataa
    14041 tactgataaa agtatagatt tctctatttc tttagttagt aaaaatcaag taaaagtaaa
    14101 tggattatat ttaaatgaat ccgtatactc atcttacctt gattttgtga aaaattcaga
    14161 tggacaccat aatacttcta attttatgaa tttatttttg gacaatataa gtttctggaa
    14221 attgtttggg tttgaaaata taaattttgt aatcgataaa tactttaccc ttgttggtaa
    14281 aactaatctt ggatatgtag aatttatttg tgacaataat aaaaatatag atatatattt
    14341 tggtgaatgg aaaacatcgt catctaaaag cactatattt agcggaaatg gtagaaatgt
    14401 tgtagtagag cctatatata atcctgatac gggtgaagat atatctactt cactagattt
    14461 ttcctatgaa cctctctatg gaatagatag atatatcaat aaagtattga tagcacctga
    14521 tttatataca agtttaataa atattaatac caattattat tcaaatgagt actaccctga
    14581 gattatagtt cttaacccaa atacattcca caaaaaagta aatataaatt tagatagttc
    14641 ttcttttgag tataaatggt ctacagaagg aagtgacttt attttagtta gatacttaga
    14701 agaaagtaat aaaaaaatat tacaaaaaat aagaatcaaa ggtatcttat ctaatactca
    14761 atcatttaat aaaatgagta tagattttaa agatattaaa aaactatcat taggatatat
    14821 aatgagtaat tttaaatcat ttaattctga aaatgaatta gatagagatc atttaggatt
    14881 taaaataata gataataaaa cttattacta tgatgaagat agtaaattag ttaaaggatt
    14941 aatcaatata aataattcat tattctattt tgatcctata gaatttaact tagtaactgg
    15001 atggcaaact atcaatggta aaaaatatta ttttgatata aatactggag cagctttaac
    15061 tagttataaa attattaatg gtaaacactt ttattttaat aatgatggtg tgatgcagtt
    15121 gggagtattt aaaggacctg atggatttga atattttgca cctgccaata ctcaaaataa
    15181 taacatagaa ggtcaggcta tagtttatca aagtaaattc ttaactttga atggcaaaaa
    15241 atattatttt gataatgact caaaagcagt cactggatgg agaattatta acaatgagaa
    15301 atattacttt aatcctaata atgctattgc tgcagtcgga ttgcaagtaa ttgacaataa
    15361 taagtattat ttcaatcctg acactgctat catctcaaaa ggttggcaga ctgttaatgg
    15421 tagtagatac tactttgata ctgataccgc tattgccttt aatggttata aaactattga
    15481 tggtaaacac ttttattttg atagtgattg tgtagtgaaa ataggtgtgt ttagtacctc
    15541 taatggattt gaatattttg cacctgctaa tacttataat aataacatag aaggtcaggc
    15601 tatagtttat caaagtaaat tcttaacttt gaatggtaaa aaatattact ttgataataa
    15661 ctcaaaagca gttaccggat ggcaaactat tgatagtaaa aaatattact ttaatactaa
    15721 cactgctgaa gcagctactg gatggcaaac tattgatggt aaaaaatatt actttaatac
    15781 taacactgct gaagcagcta ctggatggca aactattgat ggtaaaaaat attactttaa
    15841 tactaacact gctatagctt caactggtta tacaattatt aatggtaaac atttttattt
    15901 taatactgat ggtattatgc agataggagt gtttaaagga cctaatggat ttgaatattt
    15961 tgcacctgct aatacggatg ctaacaacat agaaggtcaa gctatacttt accaaaatga
    16021 attcttaact ttgaatggta aaaaatatta ctttggtagt gactcaaaag cagttactgg
    16081 atggagaatt attaacaata agaaatatta ctttaatcct aataatgcta ttgctgcaat
    16141 tcatctatgc actataaata atgacaagta ttactttagt tatgatggaa ttcttcaaaa
    16201 tggatatatt actattgaaa gaaataattt ctattttgat gctaataatg aatctaaaat
    16261 ggtaacagga gtatttaaag gacctaatgg atttgagtat tttgcacctg ctaatactca
    16321 caataataac atagaaggtc aggctatagt ttaccagaac aaattcttaa ctttgaatgg
    16381 caaaaaatat tattttgata atgactcaaa agcagttact ggatggcaaa ccattgatgg
    16441 taaaaaatat tactttaatc ttaacactgc tgaagcagct actggatggc aaactattga
    16501 tggtaaaaaa tattacttta atcttaacac tgctgaagca gctactggat ggcaaactat
    16561 tgatggtaaa aaatattact ttaatactaa cactttcata gcctcaactg gttatacaag
    16621 tattaatggt aaacattttt attttaatac tgatggtatt atgcagatag gagtgtttaa
    16681 aggacctaat ggatttgaat actttgcacc tgctaatact cataataata acatagaagg
    16741 tcaagctata ctttaccaaa ataaattctt aactttgaat ggtaaaaaat attactttgg
    16801 tagtgactca aaagcagtta ccggattgcg aactattgat ggtaaaaaat attactttaa
    16861 tactaacact gctgttgcag ttactggatg gcaaactatt aatggtaaaa aatactactt
    16921 taatactaac acttctatag cttcaactgg ttatacaatt attagtggta aacattttta
    16981 ttttaatact gatggtatta tgcagatagg agtgtttaaa ggacctgatg gatttgaata
    17041 ctttgcacct gctaatacag atgctaacaa tatagaaggt caagctatac gttatcaaaa
    17101 tagattccta tatttacatg acaatatata ttattttggt aataattcaa aagcggctac
    17161 tggttgggta actattgatg gtaatagata ttacttcgag cctaatacag ctatgggtgc
    17221 gaatggttat aaaactattg ataataaaaa tttttacttt agaaatggtt tacctcagat
    17281 aggagtgttt aaagggtcta atggatttga atactttgca cctgctaata cggatgctaa
    17341 caatatagaa ggtcaagcta tacgttatca aaatagattc ctacatttac ttggaaaaat
    17401 atattacttt ggtaataatt caaaagcagt tactggatgg caaactatta atggtaaagt
    17461 atattacttt atgcctgata ctgctatggc tgcagctggt ggacttttcg agattgatgg
    17521 tgttatatat ttctttggtg ttgatggagt aaaagcccct gggatatatg gctaaaatat
    17581 atgtttgata aaaaattatt cctgtgctac taagaaatta tttttatata ataaatattg
    17641 agatttaatt aaaagtcatg tgttattgta atacatgact tttagttaaa atttttctat
    17701 catttaataa tctattattc ttgactattt tataataaaa ttcatatatg gaaatattaa
    17761 tactaaataa ttaatagttg ataaaaaata gataatatgc taaaagcaaa aactaattta
    17821 gagccttgta actgtttatt tgcaattata aaaacatctt taaacatatt gactataata
    17881 taaaatatta actataatac aaaacaatat taattaattt tctctacagc tatccctggt
    17941 atagttatat ttccacccat agttgattca taagttatag ttccagcact tatacctctt
    18001 atagttatgt aatcatcctc taatactctt gaaggagtta ttgatttttt ataactacat
    18061 agtacaacct tatcataatt tccagacaca gctaatctta tttgcacctc atcaccatct
    18121 tcaataactt gaataacctt accttcaaat tttacctttt tgtacttata atcatcaggt
    18181 gttctagcta attggtcata agtaatacca gtatcatatc ctttcttctc ctcttcttct
    18241 tttttgcgtt gttcttcagc ctttttagct tcttcagctt ttttagcttc ttcagcttta
    18301 cgttgatttt cagcttcaat agctttcttt tcgtcgtctt tcattttgaa ccatggttca
    18361 gcatcagaca atttgctatt taaagtttct atttctgttt tttgagaatc tataactgat
    18421 gtttggttag aaatgacctc ctcatggtct tcagaacaag ctggtgagga tatattgcca
    18481 aaaaacacgc ctaaaataaa tgccagtaga gcaataccct ttgtgctctt aaagaattta
    18541 attattttct tagagcttcc ttttccttca ttactaaatt cgttaccctc atttttttta
    18601 gaaaacataa tacaatctcc cttttattag atatatttta ttttaaaagt taaaaactta
    18661 aaataactag ttgactaaaa attatatatt catattttcc taaaatatat ttcccctaag
    18721 cataatttta caaaataaag taagaagatt caatatattt tgaaaaaata agataaaaat
    18781 gtcagattat aatataattt tcttgaaggt gaattttata tattaaatta aacataaagt
    18841 attttatttg tataaaacta tattatgatg taattaagtg actcagtgat ttagaggtgg
    18901 taaaatatat atatatgaaa gagcataaat aaattgatat aataaaaaat taaatattaa
    18961 atattaacta taataaaaaa atatctggta ataggtataa tataaaaaga atataccaga
    19021 aagaaggtga agtatgggag aactacaaaa aattccaggg gtagggaaag caacagaaaa
    19081 atctttaatc atgttaggat atacaactat taaatcactt aaagatgcaa acccagcaca
    19141 aatgtatgaa aaggaatgtt taatgcgtgg acagcacata gacagatgtc agttatatgt
    19201 atacagatgt gcagtatatt ttgcatcaac tgaaaatcca gaaccagaaa aattaaagtg
    19261 gtggtattgg aaagattaaa aaatcaaaaa agttagacat aaaaaagagt tgaatatccg
    19321 ttttaaataa taaaaactaa tactcaactc ttttttagtc gtaaatttta tgtaaatcta
    19381 tttgtatggc taaatatacc catcaataaa tgtataaata tactaatcaa caaatgacta
    19441 aacatactac ctaacaaagg attaaataaa ttccttaaca aagcattcta aaacaattaa
    19501 ttagaaactg tttcttcaag ccattttcta cattgctcaa tatttaccat agggtcaaaa
    19561 ccttggtctt ttcccttatt taaaagcata tcacaaggga aagtatcaca ttcaccacaa
    19621 tgattaagtt ccttactttc acagcaagtt ttaaccttac attctccacc ccaaaatggt
    19681 ttttccatag ttgggcaacc agtgcaattt acttgttctt tttttgtaca actgtcacaa
    19741 cagacaccac atcttgattc aaacatattc aaacatcctt tccattattt aaaaatatta
    19801 taaaatgatt ttagatgaat acaaaagtgc aaatagttga ttttaggtca tctaaatgtt
    19861 aaacataaaa ttattagtac tggtaaattt ataaaaacta ttccaaaaat ctttttgaat
    19921 atttcaaaac acaataaatt acttcaaatc cccttgaata tattttacag cattatagat
    19981 tgctttaaat ctctttgaat attctacaag actatagatt agtttaaatc cctttgaata
    20041 tattttacaa cattatagat tgttttaaat ctctttgaat attctacaag actatagatt
    20101 agtttaaatc cctttgaata tttcaaaaca ccatagatta ctttaaatcc ctttgaatgt
    20161 tctataacac tataaattac ttttaacttt tatataacca taaagttttt atctggcact
    20221 ttaaatggag ttttatatca aatttgcctt ccatcgaata accaaactta atttaattac
    20281 acattccaag agtcaaatat tcattcaata tatttgataa ttctatgtta tagctataat
    20341 taaata
//
LOCUS       DAMJVK010000073        19810 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.074, whole genome shotgun sequence.
ACCESSION   DAMJVK010000073 DAMJVK010000000
VERSION     DAMJVK010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19810
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.074"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            123..1316
                     /locus_tag="PC414_002664"
     CDS             123..1316
                     /locus_tag="PC414_002664"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HDF2465052.1"
                     /translation="MDTLKELFKIGSGPSSSHTMGPQRAAERFKNENPDAESFRAILY
                     GSLAATGKGHLTDYIIEKTIAPKKVEIVWEEDIIKDFHPNGMKFEALDKDKNVTAEWT
                     VYSVGGGTIAEEGQRNSKSNSIYHLDTMDEIVKWCKENNKTLVDFVLECEPKDIKDYI
                     KTIKDAMRKSIDDGLSTDEIIPGKLLLKRRASTFYNAYKKDKSFSTLVYAYALAASEQ
                     NASGNIIVTAPTCGSAGVIPGIFFAMQDFYNYDDEKIIEALLVAGIIGNIIKTNASIS
                     GAEVGCQGEVGAACSMAAAAVAYLKGGTIDHIEYAAEIALEHHLGMTCDPVYGYVQIP
                     CIERNAMAAQRAYDAANYALLTDGSHSVSLDQVVETMKETGIDMMDKYKETAKGGLAK
                     HFFSC"
     gene            1522..2640
                     /locus_tag="PC414_002665"
     CDS             1522..2640
                     /locus_tag="PC414_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HDF2465053.1"
                     /translation="MREASYLQEQLYKHRKSLNPIAEIGRKEYKTSKYIRDYLDKLGI
                     EYEVYLETGIVGVIEGKRPKKDIAFRADIDGLHSEEGVKHLCGHDGHATILLGLIEYL
                     NDNKEDLNDNIVFIFQPAEEGPGGAKALIDEGVLRKYNIDEIYGLHIYPELPEGYVGT
                     REGYFMAQIGDIDIEIVSKSGHGAMPQNGIDGIVIASNFVSSLQTIVSRNISPIDNAV
                     LTIGRIEGGARRNIIAENIKMEGTMRCFSPEVYEKMKLRVRELARGFELAYNCKVNIN
                     IIDDYIAVKNDKDLYQEFVEAIGSDTVVELEPLMISEDFSYYQKEVPGLFFMLGSRNE
                     EKGFVNGLHNINFNFDEKICVNALNVYSKLLKYKEAID"
     gene            2771..3016
                     /locus_tag="PC414_002666"
     CDS             2771..3016
                     /locus_tag="PC414_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HDF2465054.1"
                     /translation="MKEKVQREKKITNGKNTKILTNDDIMKYEIASELGLMDKVGERG
                     WAGLTAKEAGRIGGMLTSRKKRLKKIVEEQNKKDESI"
     gene            3003..3749
                     /locus_tag="PC414_002667"
     CDS             3003..3749
                     /locus_tag="PC414_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDF2465055.1"
                     /translation="MSQYSDFAFVYDELMNEVDYNGWVKYIEDIIDSEGVKVKNILEL
                     ACGTGNLTIPLTKKNYDIAGIDISDEMLSVAREKAEKEGVELVLLQQDISELDFEISD
                     LDCVLCACDGFNYITYDDDLENVFSKTYELLKKDGIFIFDISSFYKLANILGNNMYGE
                     NREDIAYMWQNYFDEEENLIEMELTFFIKDECGKFERFEEIHQQRAYTEDEIVDMLEK
                     SGFKDIKYYGDFTFDKPSEESQRIFFVCKK"
     gene            4034..4909
                     /gene="hslO"
                     /locus_tag="PC414_002668"
     CDS             4034..4909
                     /gene="hslO"
                     /locus_tag="PC414_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HDF2465056.1"
                     /translation="MRDYVLRATSGNGQVRAFVATTRNTVEEARRLHETTKVATAALG
                     RTLTATSIMGLMMKNDSDKLTVIIKGGGPIGTIIATSDSKGMVKGYVGNPQVEVEDYP
                     NGKLNVAAAVGTEGVVKVIKDLGLREPYNGTYPLVSGEIAEDFTYYFAVSEQTPSVVA
                     LGVLTKEDEVEFAGGFIVQLMPDAEEETIAKLEENVAKLPSITNMLKEGKSPEDILNI
                     VLDGLEPKILDTCEVGFMCECSKERVKTALVAIGKKSLAQIIEEDKKAEVGCQFCNKK
                     YMYSEEELLEILKEM"
     gene            5300..6100
                     /locus_tag="PC414_002669"
     CDS             5300..6100
                     /locus_tag="PC414_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HDF2465057.1"
                     /translation="MESKTKYIGISILILLMIFLIAKNFNGKLEIPIPESNSRKFKSA
                     AVFYPQHQDDEVLWGGSAIVDAIKQCGVDNVYVVLVSDGSGVNVFKANTKFRNLTRKQ
                     KEELRNNEFKSALRELGVKPQNVIILADIDKKEGTHYELMEKTILDFEHKFKGNVTHI
                     AHHYKYDDHIMHRKNGEVLKKLKREGKIKDDLYFVKPKYVKYIPRNERAIYKAMDFDD
                     YNKVKSACYEYKVVNSKRKMYGIGYISAHSYFEHLLNDPELTSVLSED"
     gene            6628..8868
                     /locus_tag="PC414_002670"
     CDS             6628..8868
                     /locus_tag="PC414_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDF2465058.1"
                     /translation="MNNEFIILKGCKENNLNNISLKIPKRKITIFTGVSGSGKSSIVF
                     ETIAKESQRQLNERFSTFVRSFLPKYGEVKADCIENLSTPIIIDQSRLGGNSRSTLGT
                     ITDVNSFLRALYSRFGSEYIGNANMFSFNNIDGMCPHCHGLGKKLVPNMNQILDMNKS
                     LNEGAILLSGFGVGSWHWNIFAESGFFDINKRICDYSEVELEKFLHGKAEKIKIENAG
                     QTNMTYEGLMVKFNRLYLSREGEISEATKKKLSKLLIEDRCPICDGRRLNERVYRSLI
                     NGYNIADLTSMQIDELAEVIKSIDEPEAQPLIKGIIEKLNSIIDIGLGYLTLDRETSS
                     LSGGESQRIKMVKYLNSNLVDLMYIFDEPSVGLHPKDVYKLNNLLKKLRDKGNTIIVV
                     EHDPDVIKIADHIVDVGPKAGKYGGEVVYEGSYENLLTSGTLTGNALSKFLPIKESVR
                     EHSGYLEVKNCNKNNLKNVSIKIPKGVLTLVTGVAGAGKSTLIKDEFLKQNPSAVLID
                     QSPVSANSRSSLATYSGIMNNIRSIFSKTNGVNASLFSSNSEGACDNCKGSGIVEMNL
                     AFMESIKSTCNICEGKKFKKEVLEYRFQGKNIIEVLEMSVLEAIEFFNLKQIKTKLQC
                     IEKMGIGYLTLGQTLDTLSGGECQRLKLASELNKESSVYILDEPTTGLHMADIENFIS
                     IIEEIVDNGNTVIIIEHNIDVIKRADWIIELGPEGGTKGGRVIFEGIPKQLCNNKISL
                     TAKYIV"
     gene            9282..10817
                     /locus_tag="PC414_002671"
     CDS             9282..10817
                     /locus_tag="PC414_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDF2465059.1"
                     /translation="MINDILTLLSERSDFFIKLSIEHLMISIISIIIAIVLGGIIGII
                     VSEFHNSSKPILGTINFLYTIPSISMLGFLIPFSGIGNTTAVIALTIYALLPMVRNTY
                     TGITNVDKNILEAARGMGSTDFQILYKIKLPLALPVIMSGIRNMVIMTIALAGIASFI
                     GAGGLGVAIYRGITTNNTIMTITGSILIALLALIFDFILGLVEKFFQRKRKKKNRKKV
                     FAILGSLILIVIIFTSYLFNPSKTQTIHIATKPMTEQYILGEMLDILIEKNTGLNVEI
                     TQGVGGGTSNIQPAMENGEFDIYPEYTGTGWNMVLKKNELYTESMFSKLQSEYKTKLK
                     MQWTGMYGFSNSYALAVRKEVADKYNLKNITDLKDVSSKLTFGAEYDFYEREDGYTPL
                     CNTYGLNFKDTVDLDIGLKYQAINEGKIDVMPIFTTDGQYSVSDVVVLKDDKNFYPSY
                     QCGNVVRLEVLEENPELSKVFKMVGDILTDKEMAKMNYDVENNNLEPREVAREFLNKK
                     GLL"
     gene            10833..11594
                     /locus_tag="PC414_002672"
     CDS             10833..11594
                     /locus_tag="PC414_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2465060.1"
                     /translation="MTPIIQFKNIKKQYNDKTIIDNLNLDIEKGEFLTVIGSSGSGKT
                     TLLKMINGLILPDGGNILINQTDIKNEDLIKLRRRIGYCVQGSVLFPHMTVEENISYV
                     PNLLSKKNKLEVKSAVNKWMEIVGLPNDMKVRYPSELSGGQQQRVGIARALASSPEIL
                     LMDEPFGAVDEITRKQLQKEIKEIHKKTGVTIIFITHDIYEALILGTKTLVLNHGVVQ
                     QYDTPENILNTPANQFVDQLLNIRKSIIDDDNLKE"
     gene            11932..12801
                     /locus_tag="PC414_002673"
     CDS             11932..12801
                     /locus_tag="PC414_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2465061.1"
                     /translation="MDNNLITIEKTLDYIEKHLLDEMLDLNVISKEIGYSKYHLHRMF
                     TSVVGFSVHNYIQRRRLTEAARMLIFTEKPIIEIALISGYDTQRSFSRGFKKMFKRSP
                     NFFRRHQDFLPLQLKFDIHNRKKLRGDMVLDVKKVNCADINIVGYSGKVRKGFHIIGK
                     CWRDLHKNKEMIENKVDLGFLIGVSDYSNFKEIENTPTFTYIAGAQVATFGKLPNGME
                     KFVLPKSNYIVFTFIGKNEDSMQNVVEYIYQEWFPNSSCQFNENNLYDFVKYGEKTDE
                     NGLSEIQFWVPII"
     gene            13130..13381
                     /locus_tag="PC414_002674"
     CDS             13130..13381
                     /locus_tag="PC414_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HDF2465062.1"
                     /translation="MNVENELLKNLDRLHTTELGVVRIKKNLSLETNDVVNWCKTKIE
                     SPNAIINRKGKNWYISVYDCIITVNAHSYTIITAHKEKK"
     gene            13493..14272
                     /locus_tag="PC414_002675"
     CDS             13493..14272
                     /locus_tag="PC414_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDF2465063.1"
                     /translation="MNDTVKEKLAESLTSDTTDILPYLPYLLQDFWELGSSPEQMLQL
                     FRKHIPFNEDTKVLDLACGKGAVSVKLAKELNIHIKGIDLIPEFINYARRKAIEYKVD
                     ALCDFEMNDANSSVKIEHDYDCVIFGAVGDILGNYKETISKLLPTIKSKGFILIDDAY
                     VVDKSTNEQLNFERVYPTYNEWCILFKELGLKLIECIPCLELENDVEEEMNWIITRAN
                     ELIKKYPSQKDMFEQYVISQKNEYTDLQQDLVGATWILQKE"
     gene            complement(14407..15057)
                     /locus_tag="PC414_002676"
     CDS             complement(14407..15057)
                     /locus_tag="PC414_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465064.1"
                     /translation="MNIVMINGSPKVNKNNSQYFLSELKQLIQNDNKVFTFKVDSTSK
                     YPDALNAILNCDRLIIASPLYVDGIPSHLLHFLEKCEKFLKTQNKKPIPVYAIVNCGF
                     NEGEHNHLAIQMIEHWCKKINFTWVQGIGIGAGEMFGSLESVPLGKGPKKDLGIALKE
                     LSKNILSDNIHIDDSNKSTIFISPNFPRFLYKLMGNHQWNTQAKSNGLTKSDILKK"
     gene            complement(15054..15557)
                     /locus_tag="PC414_002677"
     CDS             complement(15054..15557)
                     /locus_tag="PC414_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2465065.1"
                     /translation="MRLLIHDLTDKELEKLLPKLDDNIKILPNTKEIHKCIGCFGCWI
                     KTPSECVVKDYYSNMGKLINDSDGIIIISRCCYGGFSPFVKNVLDKSINYILPYFVIK
                     DNMMRHKSRYDKQINLKAIFYGDCITEAEKETATKLVNGNAINFNIKEHSVCFYENIQ
                     DIRGESI"
     gene            complement(15606..16121)
                     /locus_tag="PC414_002678"
     CDS             complement(15606..16121)
                     /locus_tag="PC414_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDF2465066.1"
                     /translation="MLEYIILGFLMEKELSGYDLKQIMSESTSYFFDASFGSIYPALK
                     RLETKGYIHYHEVIDGSKLKKLYSITNTGKEVFLEWLKKPINFSKTKQDHLVNIFFYK
                     YLPKEIIETNLKMLITEVELLLNKLSKQKNDAEENHCVDQYTYMYATMIYGVDYYNFV
                     INWCKDLLKNL"
     gene            16401..16928
                     /locus_tag="PC414_002679"
     CDS             16401..16928
                     /locus_tag="PC414_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2465067.1"
                     /translation="MHEQIDYCERKKIRTIDIKGENMRELLKWVSIINRYNTNYIDRK
                     LADIGINNSQYFYVLHICNNPGITQDTIFEKILVNPSNITRALANLEKEGFITREPGV
                     KDKRTWHLYPTEKSLGCYDEILKITNGYVEELLSSFEKEEQELFMSMLKKVAFLAIDM
                     NEEAKLEAKENGRKI"
     gene            16912..18315
                     /locus_tag="PC414_002680"
     CDS             16912..18315
                     /locus_tag="PC414_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2465068.1"
                     /translation="MEEKYKEPLEYEKVSKLLTKYAIPSIIAMLVSALYNIVDQIFIG
                     QGVGVLGNAATNVTFPLTTICTAIALLLGIGGASKCSLELGAKNSEKATKAAGNSICL
                     MAVFGISLFIVVSIFLTPLLKFFGSTSEILPYAHTYTKITSIGLPFLIMSTGMSKLIL
                     ADGRPKASMFCMLIGAVINTILNPLFIFVFNMGIAGSALATVIGQLISFGISVHYIKN
                     FQHIKLHKKSFKLDSIIYKHIFALGSSSCFNQLAMTVTQIVMNNTLAYYGARSVYGSE
                     IPLACVGIIIKVNMIFISIIIGISQGMQPIIGYNYGAEKYNRVKEAYKFAVGSATIIS
                     VIAFLCFQFFPRQITSIFGNGNETYFIFAEHYFRTFLFLTFINGVQIVSSTFFTSIGK
                     SVLGLFTSLTRQILFLIPLIVILPMFMGIDGVMFAGPIADAAAAMVCIYFAVHEMKSL
                     TIKQNNLMSNHNKIAEV"
     gene            complement(18698..19009)
                     /locus_tag="PC414_002681"
     CDS             complement(18698..19009)
                     /locus_tag="PC414_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465069.1"
                     /translation="MKKIIKSFTSWFFLIALFEIYMHQIGQDSKSIVLIGLNPILSII
                     SRVDSFFVFMDSGMQIPCRTITGSISIYWYIASILSFLIYGIILDLIRIVISKIGNKT
                     K"
     gene            complement(19065..19658)
                     /locus_tag="PC414_002682"
     CDS             complement(19065..19658)
                     /locus_tag="PC414_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2465070.1"
                     /translation="MISDSISKRRSIRKYKNQSISHETIEKIIEAGINAPSSKNRQPW
                     RFVVITEKEKESMLKAMSKGIQNEINDNGLLPGSRQHIAGANYTVEIMKQAPVTIFIL
                     NILGKSPLEKLSPEERFYEMANMQSIGAAIQNMSLTAVELGLGSLWICDVYFAYRELC
                     EWLNTDSQLVAAISLGYPDEEPSRRPRLQLSDVTEWR"
BASE COUNT         7544 a         2152 c         3515 g         6599 t
ORIGIN      
        1 tggaaataaa aaataaattt tatgagaatt taataactaa taattaatat atttaggtta
       61 taataaatat gtgcgtaaaa tctaacaata agcaataata ccgtaaattt aggaggaata
      121 atatggatac tttaaaggaa ctttttaaaa taggaagtgg accttcaagt tctcatacta
      181 tgggaccaca aagagcagca gagagattta agaatgaaaa cccagatgca gaaagtttta
      241 gagcaatatt atatggtagt ttggcagcta cagggaaagg tcacttaact gactatataa
      301 ttgaaaaaac tatagcacct aaaaaagtcg aaattgtttg ggaagaagat attataaaag
      361 attttcaccc aaatgggatg aagtttgaag ctttagataa agataaaaat gtaacagctg
      421 aatggactgt atattctgta ggtggaggga ctatagcaga agaaggtcaa agaaacagta
      481 aaagcaatag tatatatcac ttagatacta tggatgagat agtaaaatgg tgcaaagaaa
      541 ataataagac tttggtagat tttgtgttgg aatgtgaacc aaaagacata aaagattata
      601 taaaaactat aaaagatgct atgagaaaat ctattgatga tggactaagt acagatgaaa
      661 taataccagg caagttatta ttaaaaagaa gagcaagtac attttataat gcatataaaa
      721 aagataagag cttctcaaca cttgtatatg catatgcact agcagcttca gagcaaaacg
      781 cttctggtaa tataatagtt acagcaccaa cttgtggttc tgctggagta ataccaggaa
      841 tattctttgc aatgcaggat ttctataatt atgatgacga aaaaataatt gaagcactac
      901 ttgtagctgg aataataggt aatataataa aaactaacgc ttcaatatct ggagcagagg
      961 tagggtgtca aggtgaagtt ggagccgctt gttctatggc agcagcagca gtagcatact
     1021 taaaaggtgg aactatagac catattgaat atgcggcaga aatagcactt gaacatcatt
     1081 taggaatgac ttgtgaccct gtatatggat atgttcaaat tccatgtata gaaagaaatg
     1141 caatggcagc tcaaagagct tatgatgcag ctaattatgc attattaact gatggctcac
     1201 attctgtctc cttagaccaa gttgttgaaa caatgaaaga aactggtata gatatgatgg
     1261 ataagtacaa agaaacagct aagggtggac ttgctaaaca tttcttctct tgctaaaaat
     1321 aaaattttcc aataataatt cctctgtttt gatttatgaa acaggggaat tattattaat
     1381 ggtgaattat ttaataatgc aaaatatttt tatattcaat ttagcttttt atttttaatt
     1441 agtataacat gctatataat attttaatta taaagaaata tagaaatatt ttttaataaa
     1501 ttagaaattg gaggagatta catgagagag gcatcatatt tacaagaaca actttataaa
     1561 cataggaagt cattaaatcc tattgcagag attgggagaa aagagtataa gacttcaaaa
     1621 tatataagag attatcttga taaattagga attgagtatg aagtttattt agaaacaggt
     1681 atagtaggtg taatagaagg taaaagacct aaaaaagata ttgcttttag agctgacata
     1741 gatggtcttc atagtgaaga aggggtaaaa catttatgtg gtcatgatgg acatgctact
     1801 atattacttg gattaattga gtatttaaat gataataaag aagacttaaa tgataatata
     1861 gtatttatat tccaaccagc agaagaaggt cctgggggag caaaagcttt aatagatgaa
     1921 ggagtgctta gaaaatacaa tattgatgaa atatatgggc ttcatatata tccagagtta
     1981 ccagaaggtt atgtaggtac aagagagggc tattttatgg ctcaaatagg agatattgac
     2041 atagaaatag tgtctaaaag tggacatggg gctatgccac aaaatggtat tgatggtata
     2101 gtgatagctt caaactttgt aagctcactt caaactatag tgtctagaaa tataagccca
     2161 atagacaatg cagtgttgac tattggtaga attgaagggg gagctagaag aaatataata
     2221 gctgaaaata taaagatgga aggtactatg agatgtttca gcccagaagt gtatgagaaa
     2281 atgaagctta gagttagaga acttgctaga gggtttgaat tggcttataa ctgtaaggtt
     2341 aatataaata ttatagatga ttatattgct gttaaaaatg acaaggattt gtatcaagag
     2401 tttgtagagg ctattggaag tgatactgtt gtagagttag agccacttat gatatcagaa
     2461 gatttttctt attatcaaaa agaagttcca ggattatttt ttatgttggg ttcaagaaac
     2521 gaagaaaaag gatttgtaaa tggtcttcat aatataaact ttaattttga tgaaaagata
     2581 tgtgtaaatg cattgaatgt ttattcaaaa ttattaaaat acaaagaagc aatagattag
     2641 gaagataagt tataaagatt aaaaagcata aatacctaaa tttaaaaaaa gtgtattaat
     2701 aaatacccct tacatatttg tataaattta ttttaattga caaaaataca aataagaagg
     2761 ggtgattttt ttgaaagaaa aagtacaaag agaaaagaag ataactaatg gcaagaatac
     2821 taaaatattg acaaatgatg atataatgaa atatgagata gcctcagaac taggtcttat
     2881 ggataaagtt ggtgaacgtg gatgggcagg acttacagct aaggaagcag gaagaattgg
     2941 aggtatgctt acatcacgca aaaagaggct taaaaagatt gtggaggagc agaataaaaa
     3001 ggatgagtca atatagtgat tttgcgtttg tatatgatga acttatgaat gaagttgatt
     3061 ataatggttg ggttaaatat atagaagata taatagatag tgaaggtgta aaagtaaaaa
     3121 atatattgga attggcatgt ggcacgggta atcttacaat tccattgact aaaaaaaatt
     3181 acgatatagc tggtatagat atatctgatg agatgcttag tgtagcaaga gaaaaggcag
     3241 aaaaagaagg agtagaatta gttttacttc aacaagatat atcagagctt gattttgaaa
     3301 tatctgattt ggactgtgta ctttgtgctt gtgatggatt taattatata acttatgatg
     3361 atgatttgga aaatgtattt tcaaagacat atgaattatt aaagaaagat ggaatattca
     3421 tatttgatat aagttctttt tataaattag ccaacatatt gggaaataac atgtatggag
     3481 aaaatagaga agatatagcg tatatgtggc aaaattactt tgatgaagaa gagaatctaa
     3541 ttgaaatgga acttacattc tttattaaag atgaatgtgg aaagtttgaa agatttgaag
     3601 agatacacca acaaagagca tatactgaag atgagatagt tgatatgctt gaaaaatctg
     3661 gatttaagga tataaaatat tatggggatt ttacatttga caaacctagt gaagaaagtc
     3721 aaagaatctt ctttgtatgt aaaaaataag atattcttga ctatataaga taattattaa
     3781 aaataaccat gatataaaaa tatataaaat gcagttttat agattaaaag taaagtgatg
     3841 atattttatg atttttattt tctaataatt ataaagtttt atagataaaa attaaaatat
     3901 aataactatt gcttagtaaa attaaaataa aatgggtttt gcccaaggtt aagatagata
     3961 agtatttata gattaaaatt aaggtagtaa tataataaaa ataagacaat taacgggaca
     4021 tggaggaata ttaatgagag attatgtact aagagcgaca tcaggaaatg gtcaagttag
     4081 agcatttgtc gcaactacta gaaatacagt agaagaagct agaagattac atgaaactac
     4141 aaaagtagca acagcagcac ttggaagaac attaacagcc acatctataa tgggattaat
     4201 gatgaaaaat gatagtgata aattgactgt aataataaaa ggtggaggtc caattggaac
     4261 tattatcgcc acatcagact ctaagggaat ggtaaaagga tatgtaggaa atccacaagt
     4321 agaggttgaa gactatccaa atggaaagtt aaatgtagca gcagcagtag gtacagaagg
     4381 tgttgtaaaa gttataaaag acttaggtct tagagaacca tataatggga cttatccatt
     4441 ggtaagtgga gaaattgcag aagactttac ttattatttt gctgtatcag agcagactcc
     4501 atcagtagta gctttaggtg ttcttacaaa agaagatgaa gtagagtttg ctggaggatt
     4561 tatagttcaa ttaatgccag atgcagaaga agagactatt gctaagttag aggaaaatgt
     4621 agctaaatta ccttctataa ctaacatgtt aaaagaagga aaatcaccag aggatatatt
     4681 aaatattgtt ttggatggtt tggaacctaa aatacttgat acctgtgaag ttgggtttat
     4741 gtgtgaatgt tcaaaagaaa gagtaaaaac tgctttggtt gcaattggta aaaaatcatt
     4801 ggctcaaata atagaagaag ataaaaaagc agaagttggt tgccagttct gtaataagaa
     4861 gtatatgtat agtgaagaag agttgcttga aattttaaag gaaatgtaga ctataaaaat
     4921 agttgtattg atataagtaa tgttatagta atatagtaaa gtcgtattga tatagtaatt
     4981 aaagtataat ttattaataa tagacatatt tatattaaaa aataagttta ataaattttt
     5041 aagcttaata aattttaaga attaagaagc tttctggctg taatgtttgc aatgttgcag
     5101 ttagaaggct tttttatttt attatagttc gtgtcaaaga ttgtatgaaa caataaaata
     5161 ttgtaaaata atttgtatat ttagacctga ttgacacaaa ggtttataag atatttgagt
     5221 tgattatacg aaattctatg aagtagtaac cctgtgactt taatcatggg aggttcagaa
     5281 gaaggagaaa attttaaata tggaaagtaa aacgaagtac ataggaataa gtatattaat
     5341 attattgatg atatttttaa ttgcaaagaa ttttaacgga aaacttgaaa tacctattcc
     5401 tgagagtaat agtagaaaat ttaaatcagc agcagtgttt tatcctcaac atcaagatga
     5461 tgaggtttta tggggaggaa gtgccattgt agatgctata aaacagtgtg gtgttgataa
     5521 tgtatatgtg gtattagtat cagatggttc tggagttaac gttttcaaag ctaacacaaa
     5581 atttagaaat ttaactagga agcaaaagga agaacttaga aataacgagt ttaaatcagc
     5641 tttgagagag cttggagtga aacctcaaaa tgtgattata cttgcagata tagataaaaa
     5701 ggaaggcaca cattatgaat taatggaaaa gactatattg gattttgaac ataagtttaa
     5761 aggcaatgtg acccatatag cccatcacta taagtatgat gaccatataa tgcatagaaa
     5821 gaatggggaa gttttaaaga aacttaaacg tgaaggtaag ataaaagatg acttgtattt
     5881 tgttaagcca aaatatgtaa agtatatacc aagaaatgaa agagctatct ataaagcaat
     5941 ggattttgat gactacaata aggttaagag tgcttgttat gaatataagg ttgtaaattc
     6001 aaagcgtaaa atgtatggta taggatatat ttcggcacat agttattttg aacatcttct
     6061 aaatgaccct gaacttacgt ctgtattaag tgaagattaa tttcttataa gtagtaagtt
     6121 atgtttgata agttgattgg aattttctat agttttgaag gtattggaaa tttagtttta
     6181 tttaaagatt taaagataga ggtttttagt gtttaaaatt ttaaagttaa gttgaataat
     6241 tgtatagaat tatttgtgaa agaagaaaca gtgtaaaaca tagtatctta tttatggttt
     6301 taaaatcata ggcaagatac tttttttatg taactaatac cattttgaga ttattaactt
     6361 aagaatttta tataatttaa cactcatata taatgatgat atttaggtag aatagatgta
     6421 attacgctta aataaaataa ctgtattttt tataaaaatc caaaccctac actatactac
     6481 cagataaaat ctacatgcta taatttgacc tgtgtttata aaaaaaggag taatctaata
     6541 aaatagtatt tcacttatgt ataactaaca actattatta cgattttctc taaataattt
     6601 aaagaaaaat aatgcaggag gtaaaatatg aacaatgaat ttataatttt aaaaggttgc
     6661 aaagagaaca atttgaacaa tatttcctta aaaataccaa aaagaaaaat aaccatattc
     6721 acaggggtgt cgggttctgg aaagtcatct attgtatttg agacaattgc aaaagaatca
     6781 caacgccaac taaatgaaag gtttagtaca tttgtaagaa gttttttacc taagtatgga
     6841 gaggttaaag ctgattgtat agaaaatttg tcaacaccaa ttatcataga ccaaagtaga
     6901 cttggtggaa attcaaggtc aactctaggt actattacag atgtaaattc ctttttaaga
     6961 gctttatatt caaggtttgg tagtgagtat ataggtaatg ccaatatgtt ttcttttaat
     7021 aatattgatg gtatgtgccc acactgtcat gggcttggaa aaaagctagt tcctaatatg
     7081 aaccaaattc ttgatatgaa taaatcatta aatgaaggtg ctatattgct ttcagggttt
     7141 ggagtgggtt cttggcattg gaatatattc gctgaatcag gattttttga tattaataag
     7201 aggatatgtg attattcaga agtagaattg gaaaagtttt tgcatggtaa agcagaaaag
     7261 attaagatag aaaatgctgg tcaaactaac atgacatatg agggattaat ggttaaattt
     7321 aatcgcctat acttaagtag agagggagaa atatctgaag ctactaagaa aaaattgagt
     7381 aaacttctta tagaagatag atgtcctata tgtgatggaa gaagacttaa tgaaagagta
     7441 tataggtctt taattaatgg ttataatata gcagatttaa ctagtatgca aattgatgaa
     7501 ttggctgaag ttattaaaag tatagatgag ccagaagcac aacctttaat taaaggaata
     7561 atagaaaagt taaacagtat catagatata ggtcttggat atttaacact agatagagag
     7621 acatcttctc tatcaggagg ggaatctcag cgtataaaga tggttaagta tttaaatagt
     7681 aatcttgttg atttaatgta tatttttgat gaaccaagtg taggacttca tccaaaagat
     7741 gtgtataaat taaataatct tttaaaaaaa ttgcgtgata aagggaatac aattatagtt
     7801 gttgaacatg acccagatgt tattaagatt gcagaccata ttgtagatgt aggaccaaag
     7861 gcaggtaagt atggtggaga agttgtgtat gaaggcagtt atgaaaatct acttacaagt
     7921 ggtactttga cagggaatgc tttaagtaaa tttcttccta ttaaagaaag tgtaagagaa
     7981 catagtggat atttagaagt taaaaattgt aataaaaaca atttaaaaaa tgtatctatt
     8041 aagataccta aaggtgtatt gactttagtt actggagtag caggagcagg taaaagtaca
     8101 ttgattaagg atgaattttt aaagcaaaat cctagtgctg tacttataga ccaaagccct
     8161 gtttcagcaa attcaaggtc gagtttggca acatatagtg gaattatgaa caacataaga
     8221 agcatatttt caaaaacaaa tggcgtaaat gcttctttat tcagttcaaa ttcagaagga
     8281 gcttgtgata actgtaaggg ttctggaata gtagaaatga atcttgcttt tatggagagt
     8341 ataaagagta cctgcaatat ttgtgaaggt aaaaaattta aaaaagaagt tttagaatat
     8401 aggtttcaag ggaagaatat aattgaagtt cttgaaatgt ctgttttaga ggcaattgag
     8461 ttttttaatt taaaacagat taaaacgaaa ttacaatgta ttgaaaaaat gggtattgga
     8521 taccttactt taggacaaac tcttgatact ttgtctggag gagagtgtca aagattaaag
     8581 cttgctagtg aattaaataa ggaaagttca gtgtatatat tagatgaacc aacaacaggt
     8641 cttcatatgg ctgatattga gaattttata agtataattg aagagattgt agataatgga
     8701 aatacggtca taataattga acacaatata gatgtaatta aaagagctga ttggattatt
     8761 gagctgggac ctgaaggagg aacaaaaggc gggagagtaa tatttgaagg tattccaaaa
     8821 caattatgta acaataaaat atcattaaca gcaaaatata tagtataata aataaaatat
     8881 aataaaatta taatgatatt ttaataaata taatgggtat ataaaatgat aattaaactt
     8941 aacagtaata aataatgtga gaaatattca aatttaatta aataaagtgt cagaaatttg
     9001 tttaaatttt agtattttaa cggttctgac cttttttctt atttttatgg agaatatcga
     9061 ggatggagtc tctaatagtt acttttttgt gtctacttgc aagtttgatt aaaaaatggc
     9121 tatacacact tgtgacttca gtcatgggag gttcagatta taatcttaat atataatttg
     9181 tgaaataatt tgatgacgat ttgctgatgt ataaacaata aagattaact acattaaaat
     9241 ttaaatcttg ttaagtaaaa cttataaaag gagagagatg gatgataaat gatattctaa
     9301 ccttattatc tgaaagaagt gattttttta taaagctatc catagaacat ttgatgattt
     9361 ctattatttc tataataatt gcgattgttt tgggtggtat aattggtatt attgtaagtg
     9421 aatttcataa ttcttctaaa cctatattgg gaactatcaa ttttctatac accattccat
     9481 ctatttctat gctaggattt ctgataccat tttccggaat tggaaatact actgctgtta
     9541 ttgcacttac catttatgca ttattgccaa tggttagaaa tacatatact ggaattacta
     9601 atgtggataa aaatattctt gaagcagcta ggggaatggg aagtacagat tttcagatat
     9661 tgtataaaat aaaacttccg ttagccctac ctgttattat gtctggtatt agaaatatgg
     9721 tgattatgac aattgcatta gctggtatcg cttcatttat aggtgctgga ggcctaggtg
     9781 tagccatata tagaggaatc acgacaaata acacaataat gacaattact ggaagtatac
     9841 ttattgctct attagcttta atatttgatt ttattttagg gctggtagaa aaattttttc
     9901 aacgaaaaag aaaaaagaaa aataggaaaa aagtttttgc cattctagga agccttattc
     9961 ttatagtaat tatctttacg tcttatttat ttaatccttc aaagactcaa actattcata
    10021 ttgctactaa acctatgact gaacaatata ttcttggtga gatgttggat attttaattg
    10081 aaaaaaacac aggtctaaac gtggaaatta cacaaggtgt aggaggggga acatctaata
    10141 tccagccagc tatggagaat ggagaatttg acatttatcc agagtataca ggtacaggct
    10201 ggaatatggt acttaagaaa aatgaattat atactgaaag tatgtttagt aaactacagt
    10261 cggaatacaa aactaaactt aaaatgcaat ggactggtat gtatggtttt tctaatagct
    10321 atgcattagc agttagaaaa gaagttgcag ataaatacaa tttaaaaaat ataacggact
    10381 taaaagatgt atcttctaaa ttgacatttg gagctgaata tgacttttat gagagagagg
    10441 atggatatac tccattgtgt aatacatatg gactgaactt taaagataca gttgatttag
    10501 atattggatt aaaataccaa gctatcaatg aaggtaagat tgacgtaatg cctatattta
    10561 caactgatgg tcagtatagt gtttcagatg ttgtagttct aaaagatgat aaaaatttct
    10621 acccatctta tcaatgtgga aatgttgttc gtttggaagt attagaggaa aatccagaac
    10681 tatcaaaggt atttaagatg gttggagata ttttgactga taaagaaatg gctaaaatga
    10741 attatgacgt agaaaacaac aatttagaac ctagagaagt tgctcgtgag tttctgaata
    10801 agaaaggtct tttataaatt ggaggaaata taatgacacc tattatacaa tttaaaaata
    10861 ttaaaaaaca atataatgat aaaactataa tagataatct taatttagat atagaaaaag
    10921 gagagttttt gactgtaatt ggttcgtctg gttctggtaa gacaacactc ttaaaaatga
    10981 ttaatggtct gattttgcca gatggaggaa atattttgat aaaccaaaca gatatcaaaa
    11041 acgaggactt aattaaatta agaagaagaa taggatattg tgtacaggga agtgttcttt
    11101 ttcctcatat gactgttgaa gaaaatattt catatgtacc aaatctccta agtaaaaaga
    11161 ataaattaga agtaaaaagt gctgtaaaca agtggatgga aatagtggga ttgccaaatg
    11221 atatgaaagt gcgttaccca tcagaattgt caggtggtca gcaacaacgt gtagggattg
    11281 caagagcttt agcttcttct ccagaaattt tattaatgga tgaacctttt ggtgcagttg
    11341 atgagattac acgaaagcaa ttacaaaaag agataaaaga aattcataaa aaaacaggag
    11401 ttacgattat ctttattacg catgatattt atgaagcttt gattttggga acaaagactt
    11461 tggtattaaa tcatggagtt gttcaacagt atgatactcc tgagaatatt ttaaatactc
    11521 ctgctaacca atttgtagac cagttattga atataagaaa atctataata gatgatgata
    11581 atttaaaaga ataaatttaa atttaaaatc aaattgttgt gataaaaact attgatttag
    11641 agctaacttc aagttgtata cttgaagtgg aaaagttaga ttaaggtata gaagggcatt
    11701 tagcagttgg ctgtagacga ggtaactgct gaaggtgctt tcaataaatg gctagaacct
    11761 gtatctgttg aagattataa taaattgaaa taagaagctc aatatacaga gtagataggc
    11821 acttcaaata gaagtgtctt ttgtgtgcca taaaataaat caaaatatta gcaaagaatg
    11881 tgaaaaacaa aataaaataa tatgctatct ttaaaatagg agggatgcta tatggataac
    11941 aatttgatta ccattgaaaa aactttagat tatatagaaa aacatctttt agatgagatg
    12001 ttagatttaa atgtaatttc taaagagata ggctattcaa aataccattt acatagaatg
    12061 tttacatcag ttgtcggatt tagtgtacat aattatatac aaagacgtag acttactgaa
    12121 gcagcgagaa tgctaatttt tacagaaaaa ccaattattg agattgcttt aatctcgggg
    12181 tatgatacac aacgctcttt ttcaagaggg tttaaaaaaa tgtttaaacg tagtcctaat
    12241 ttttttagaa gacatcaaga ttttttacca ctacaattaa aatttgatat tcataataga
    12301 aaaaaattac gtggagatat ggttttagat gttaaaaaag ttaattgtgc agatataaat
    12361 attgttggct attctggtaa agtaagaaaa ggatttcata ttattggaaa atgctggagg
    12421 gatttgcata aaaataaaga gatgatagaa aataaggtag atttaggttt tttaattgga
    12481 gttagtgatt atagcaattt taaagagatt gagaatactc ctacatttac atacattgca
    12541 ggtgctcaag tagctacttt tggcaaatta cctaatggaa tggaaaaatt cgtattaccg
    12601 aaaagtaatt atattgtttt tacttttatt gggaaaaatg aggatagtat gcaaaatgtt
    12661 gtagaatata tctatcaaga atggttccct aattcaagtt gccagtttaa tgaaaataat
    12721 ttatatgact ttgtaaaata tggagaaaaa actgatgaga atggtttgag tgaaattcag
    12781 ttttgggttc caatcattta aaagaaggta ttatgtataa agtagatgga tttatatagg
    12841 caaagtttta cagcagtatg tatttataaa taatttatag tttgtaggaa agaaataatt
    12901 ttactataag attatgataa aatacacaat actatgacaa aatagtgtta aataatagat
    12961 ttaattttgc ataggaaaag ttctataatg gtgtgtattc ataaataatt tataatctgt
    13021 aggaaagaaa tagttttact ataagattat gataaaatac acaatactat gataaaatag
    13081 tactaaataa tagatttaat tttgcaaagg ataaggtgtt ttaataaaaa tgaatgtaga
    13141 aaacgaatta cttaaaaacc tagatagatt gcatacaaca gagttaggcg ttgtaagaat
    13201 taagaaaaat ctttctttgg agacaaatga tgtagtgaac tggtgtaaaa ctaaaataga
    13261 atctccaaat gccattatta atagaaaggg aaaaaactgg tatataagtg tatatgattg
    13321 tattataaca gtaaacgctc acagttatac aattattaca gctcataaag aaaagaaatg
    13381 aattgcaaaa tacaaaggtc aaaagcattt tccactgttt gtagtaccta tgaagaagta
    13441 tataaaataa tattactttt gaataataat ttaaattttg gaggttgaaa aaatgaatga
    13501 tactgtaaaa gaaaaattgg cagagtcact aacttctgat actactgata ttttacctta
    13561 tttgccttat ttattacaag atttttggga attaggaagt tcaccagaac aaatgttaca
    13621 attatttaga aagcatatac cttttaatga ggatacaaaa gttttggatt tagcttgtgg
    13681 aaaaggtgct gtatctgtga agcttgcaaa agagttgaat atacatatca aaggtattga
    13741 tttgatacct gaatttatta attatgctag aagaaaggct attgaatata aggttgatgc
    13801 tttgtgtgat tttgaaatga atgatgctaa tagttctgta aaaattgagc atgattatga
    13861 ttgtgtgatt tttggagctg taggggatat tttaggtaat tataaagaaa ctataagtaa
    13921 gctattacct acaataaaaa gtaaggggtt cattttaatt gatgatgcat atgtagtaga
    13981 taaatctaca aatgaacagc ttaattttga gagggtgtat cccacttaca atgagtggtg
    14041 tatattgttc aaagaattag gtctgaagct aattgaatgt ataccatgtt tagaacttga
    14101 aaatgatgtt gaagaggaaa tgaattggat tataactcgt gcaaatgagc taattaaaaa
    14161 atatccttct caaaaagata tgtttgaaca atatgttatt agccaaaaaa atgaatatac
    14221 tgatttacag caggaccttg ttggagcaac ttggatttta caaaaagaat aagataaata
    14281 ttagactaga taatcaatgt tatagtgaat tatcccatgt ctagtgaata taaataacat
    14341 aaaaaaccgt caagatgaat agtttattcg tgcttgacgg tttttagtat ttgttgaagt
    14401 aaaattttat ttctttagta tatcactttt agtcaatcca tttgatttag cttgagtatt
    14461 ccattgatga ttccccataa gtttatatag gaatctaggg aagttaggtg aaataaaaat
    14521 agtacttttg ttggaatcat ctatatgtat attatcacta agtatatttt ttgaaagttc
    14581 ttttaatgca attcctaaat cttttttagg tcctttaccc aaaggtacgg attctagtga
    14641 accaaacatc tccccagctc caattccaat accttgaacc catgtaaagt tgattttttt
    14701 acaccaatgc tctatcattt gaattgccaa atgattatgt tcaccttcat tgaatccaca
    14761 gttaactatt gcatacactg gaataggttt tttattttgt gtttttaaaa atttttcaca
    14821 tttctctaaa aaatgaagta aatgagatgg aataccatct acataaagag gagatgcgat
    14881 tattaatcta tcacaattaa gtatggcatt aagagcgtca ggatacttag atgtgctatc
    14941 tactttaaat gtaaaaactt tattatcatt ttgaataagt tgttttaatt cagataaaaa
    15001 atactgtgaa ttatttttat ttacttttgg actaccattt atcattacta tattcatatg
    15061 gattcacctc ttatatcttg tatattttca tagaaacaaa cagagtgttc ttttatattg
    15121 aaatttatag catttccatt tactaatttc gtagcagttt ctttttcagc ttctgtaata
    15181 caatctccat agaatatagc ttttaaattt atctgtttat catatctgct tttatgacgc
    15241 atcatattat cttttattac aaagtatgga agaatataat taatgctttt atcaagtacg
    15301 tttttgacaa aagggctaaa ccctccgtaa cagcatctgc ttattataat aattccatca
    15361 gagtcattta taagtttacc catgttgctg taataatctt ttacaacaca ttcagaagga
    15421 gttttaatcc agcaaccaaa acaaccaata catttgtgga tttcttttgt gtttggaagt
    15481 atttttatat tgtcatcaag tttgggtaat aacttttcta attctttgtc agttaaatca
    15541 tgtattaaaa gcctcataaa gtccatctcc ttttattatg tatgtttact tctaaaaatt
    15601 atatcttata gatttttcag taaatcttta caccaattaa taacaaaatt ataatagtca
    15661 actccataaa tcattgttgc atacatatat gtatattggt caacgcaatg attttcttct
    15721 gcatcatttt tttgtttact taatttattt agaagtaatt ctacttcagt tattaacatt
    15781 tttaagtttg tttcaattat ttctttgggt agatacttgt aaaagaatat attaacaagg
    15841 tggtcttgct ttgttttaga aaaatttata ggtttcttta accattcaag aaatacttct
    15901 ttaccagtat tagttatgga atataatttt tttaatttgc taccatctat aacttcgtga
    15961 tagtgtatat atccttttgt ttctaatctt ttaagtgcag gatagatact tccaaagctg
    16021 gcgtcaaaaa agtacgaggt actttctgac attatttgtt ttaagtcgta tccacttaat
    16081 tctttttcca ttaaaaatcc taaaataatg tattctaaca tattacctcc tggtatcaat
    16141 tagatatata tcgattcgat atatttatta ttacagataa taaatcttac gtcaagatgc
    16201 tttttaagat aagctaaaaa gaattaggag ttattgataa atatgcaaaa aaattaatat
    16261 aaaaggtaat taaatatcaa tgaagattta aaaatagtta aatcaaagta agagtctaat
    16321 atataaaatt tatagtaact ttatggttta aatataaact tgcgtgctat tgacaaagta
    16381 taaaattagc agtaaaataa ttgcatgagc aaatagatta ttgcgaacgc aaaaaaataa
    16441 ggacaataga tattaaagga gagaatatga gagagctatt aaaatgggtt tccattataa
    16501 atagatacaa tacaaattat attgacagga aattggcgga tattggaatc aataatagcc
    16561 aatattttta tgtattacac atatgtaata atccaggtat tacacaggac actatatttg
    16621 agaaaattct tgtgaatcct agtaatataa caagagcttt ggcaaattta gaaaaagaag
    16681 gctttataac aagagaacca ggtgtaaagg ataaaagaac atggcattta tatcctacag
    16741 aaaaatcttt aggatgttat gatgaaatac taaaaataac aaatgggtat gttgaggagt
    16801 tgttgagttc atttgagaaa gaagagcaag agttgtttat gtcaatgtta aagaaagttg
    16861 catttttggc tattgatatg aatgaagaag caaaattgga ggcgaaggaa aatggaagaa
    16921 aaatataagg agccattgga atatgaaaag gtttcaaagt tattaactaa gtacgcaatt
    16981 cctagtatta tagcaatgtt agtaagtgct ttatataaca ttgttgacca aatatttatt
    17041 gggcaaggtg ttggtgtatt gggtaatgct gctacaaatg tcacttttcc actaacaaca
    17101 atatgtactg caattgcatt attgttagga attggaggag cgtctaagtg ttcattggaa
    17161 ttaggtgcta aaaattcaga aaaagctaca aaagcagcag gaaattctat atgcttgatg
    17221 gctgtttttg gaataagttt atttatagta gtatcaatat ttttaacacc actattaaaa
    17281 ttctttggct ctacatcaga aatactacct tatgcacaca catatacaaa aattacatca
    17341 attggattac catttcttat aatgtccact ggaatgagta agttgatact tgcagatggg
    17401 agaccaaaag cttctatgtt ttgtatgtta ataggagcag ttattaatac aattttgaat
    17461 ccattattca tttttgtttt taatatggga attgctgggt ctgctttagc aactgttatt
    17521 gggcaactta tatcttttgg aataagtgtt cattatataa agaattttca acatattaaa
    17581 ttacataaga aaagttttaa attagatagt ataatttata aacatatatt tgcgctaggt
    17641 tcaagctcat gttttaatca attagcaatg actgtaacac agatagttat gaataataca
    17701 ttggcatatt atggggctag gtcagtttat ggaagtgaga ttccacttgc ttgtgtcggt
    17761 atcattatta aagtaaatat gatttttata tctataatta ttggtatatc acaaggtatg
    17821 caaccaatta tagggtataa ttatggggct gaaaagtata atcgtgtaaa agaagcatat
    17881 aagtttgcag tgggaagtgc aactattata tctgttattg cattcttatg tttccaattc
    17941 ttcccaagac aaattacgtc tatatttggt aacggaaatg agacttattt tatatttgca
    18001 gaacattatt ttagaacttt cttgttctta acatttataa atggtgttca aatagtatca
    18061 tcaactttct ttacatcaat tgggaaatca gtgttgggac tttttacttc tttgactcgt
    18121 caaattttat ttttaattcc attgatagta attttaccaa tgtttatggg aattgatgga
    18181 gtaatgtttg caggaccaat agcagatgca gcagcggcaa tggtatgtat atactttgca
    18241 gttcatgaaa tgaaatcatt gactataaag caaaataatt tgatgagtaa ccataataag
    18301 attgcagagg tataagacaa tatttaagaa gtatagaaaa ttaatttaaa aaatataata
    18361 aattaattta ggaagtgtag aaaattaatc taaagaagta taataaacta atttaggaag
    18421 tatagaaaat taatctaaag aagtgtaata aactaattta ggaagtatag aaaattaatt
    18481 taaagaagta taataaacta atttaagaaa tatagaaaat taatttaaag aaatacaata
    18541 cattaatttt taagtaacat taaattaatg taagtaaaat aatgttcata tgaatattta
    18601 aaggtgcatt ttctgaagct aagtatatgt tgtagagtgt tatgtctctc tatcctgtga
    18661 agtttaatag ctcaagaaaa tgcactattt atgatgatta ttttgtttta tttccaattt
    18721 tggaaataac tattctaatt aaatctaaaa ttataccata aataagaaaa gaaagtatag
    18781 atgctatgta ccaataaatc gaaattgacc ctgtaattgt tctacatggg atttgcatac
    18841 ctgaatccat aaaaacaaaa aaactatcaa ctctggaaat aatagacaaa ataggattta
    18901 aacctattag aacaatactt tttgagtctt gaccaatttg atgcatataa atctcaaata
    18961 atgcaattaa aaaaaaccaa gatgtaaaag atttaataat ttttttcatc aaaatacctc
    19021 acataatata aattctgttt gcactatttg cttactacaa ctatttatct ccattcagtc
    19081 acatcactta attgtaatct aggtcttctt gatggttctt catcaggata acctaaagat
    19141 attgctgcca ctaattgact atctgtattt aaccattcac ataattcacg atatgcaaag
    19201 tatacatcac aaatccaaag actacctaga cctaattcca cagctgtaag cgacatattc
    19261 tgaattgcag ctccaattga ttgcatattt gccatttcat agaatcgttc ttcaggggat
    19321 agcttttcta aaggtgattt acctaatata tttaaaataa aaattgttac tggagcttgt
    19381 ttcatgattt caactgtgta atttgcacca gcgatatgtt gacgacttcc tggaagaagc
    19441 ccattatcat taatttcatt ttgaatacct ttgctcatgg ctttaagcat agattctttt
    19501 tccttttcag tgatgacaac aaaacgccat ggttgcctgt ttttagatga aggtgcattt
    19561 attccagctt caattatttt ctctatagtt tcatgtgaaa tgctttgatt tttatatttt
    19621 ctgatacttc gtctttttga tatagaatca cttatcattt taagtaacct ccgtttattt
    19681 tttggaaaaa tctatttaat ttttattttc tattttaatt attttagcat taatcagaaa
    19741 aaaagtcatt gattttagta tacaattatt aatgattata ttaagaattg tatcaaaaag
    19801 tatgtgatta
//
LOCUS       DAMJVK010000074        19339 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.075, whole genome shotgun sequence.
ACCESSION   DAMJVK010000074 DAMJVK010000000
VERSION     DAMJVK010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19339
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.075"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            335..913
                     /locus_tag="PC414_002683"
     CDS             335..913
                     /locus_tag="PC414_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF2465071.1"
                     /translation="MGITLSKGQKVDLTKGNPGLKNILVGLGWDTNKYDGGFDFDLDT
                     AAFLTGASGSVTNDGDFVFYNNLKHTSGAVEHLGDNRTGEGDGDDEQIVVDLSKIPGE
                     ISKISFTVTIHDATERRQNFGQVSNSYIRIVDKDTNEELIKYELGEDFSIETAIVVAE
                     IYKHNGEWKFNALGSGFEGGLAALCGNFGINL"
     gene            952..1530
                     /locus_tag="PC414_002684"
     CDS             952..1530
                     /locus_tag="PC414_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF2465072.1"
                     /translation="MAIVLKKGQKIDLTKGNPGLKNIKLGLGWDTNSFDSGYDYDLDV
                     SVFMVGESQRVEKDEDFIFYNNLKHPSGAVEHLGDNRTGEGDGDDEEILVNLETMPKH
                     IQKIAVTVTIYEAAERRQNFGQVSNSYIRVLNSENDEEILRYDLGEEFSIETAITVCE
                     IYKYNGEWKFSAVGAGFEGGLEALCRNYGLNV"
     gene            1616..2245
                     /locus_tag="PC414_002685"
     CDS             1616..2245
                     /locus_tag="PC414_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF2465073.1"
                     /translation="MSINLSKGDKIDLKKSNPGLSNILVGLGWDPVQQSGGGFFKSLF
                     GGGQADIDCDASVFMLNQEGKLSGIKDLIYFGNLKSACKSVLHTGDNLTGEGTGDDEQ
                     ILVNLDKVPSNIHKLLFVVNIYNCVDRKQHFGMIENAYIRVEDQGNKKEIAKYNLSDN
                     YSEKTTLIVGAIYRKDGSWQFKAIGEGTKDAGLKEVMQNLDRIECAYGI"
     gene            2235..4994
                     /locus_tag="PC414_002686"
     CDS             2235..4994
                     /locus_tag="PC414_002686"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HDF2465074.1"
                     /translation="MEFNNDIKYYNVDKEEVTKDLSVNPEKGLSESEVKTRREKYGLN
                     EFTPKEEGSFWDDLKESLSEPMIVILIIAAVVSAVIGETHDAIGIVGAIAIGIAIGMI
                     TEGRSKKAAEALSKLTENIEVKVLRDGEVHQISKSELVPGDIVYIETGDMVPADGRLI
                     ESINLKIREDMLTGESDDVSKKCDVVVSMEEIESKGAVVVQEPIPAKQINMVFGGTLV
                     AYGRGALVVTSIGDNSEMGKIAQNLSETDEQTPLQIKLGNLGGLIAKVSSAVAGLLFI
                     FMVFQMISKNVLNVDMSGILPFLESIDPVKTAFTVCVALIVAAVPEGLPTMINMTLAI
                     TMQKMAKINALVTKKEACETIGSVSVICSDKTGTLTENRMTVEVAYVDGRYIESSEEE
                     INSYFEENCMINSTADVEHNDGDIKYLGSATECALLLYYKNVDYRQVRKNSNIVAQNP
                     FTSDSKRMTSVIGQDNHHVLLSKGAPEVLLELCSHIQRGKDIVPLTENIKHEILEEIK
                     KLQIKSMRTLGFAYKEISQAEEEAAVTAESDAMNVSAMENNLVFSGFVGIRDPLRKDV
                     IESVNTANKAGVSVKMLTGDNINTARAIGEELGLLKNNMRVVEASYIDTLDDEELKQE
                     IQSISIVARSKPDSKMRIVSALQKSGDVVAVTGDGINDAPALSKADVGIAMGISGTEV
                     SKNAADIILTDDSFSTIVKGIKWGRGIYDNFQRFVQFQLTVNVIAFLVAIISQVMGQE
                     MPFTTIQLLWVNIIMDGPPALALGLEPVRDHVLNRKPVDRKANIISKSMVYTIVLNAF
                     YITAILMLQSTFNFLGVDEAHKGTVMFALFAFSALFNAFNCREFNADSIFPNFTHNKL
                     ALQIIVLTGIAQVIFTQLFQDFFNSVAMDFMTWVKVLCVAASVIVVNEVVKCIIRLVK
                     LKKTA"
     gene            5081..6643
                     /locus_tag="PC414_002687"
     CDS             5081..6643
                     /locus_tag="PC414_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceG family protein"
                     /protein_id="HDF2465075.1"
                     /translation="MGDSKVFEKIFSSQSDRGNYTPSKGYLSYFISYIGLEDEVLYNL
                     EIFKTKQNIDSKKDIALFTDVIANPSDFDIINYFKSGLQKYRTSMEDVDINILGFEEI
                     DYKIKQAMDRVLKEEEKEFTNDRVKQNFIVKIMAWIKIYIGALDINKNEAPKVIFYGD
                     IKKHEVYLLLILYLAGFDVLYLNPNSKSNIDILKSERYNIEFEEANIIEEKISFEERV
                     ILGEKIDKSSVKKAFTVGAEASKRISEELLNDAGFIKPWQLQDRKIKNLLLSSTVDEI
                     SIYWNQPLKLRPGFKFNDAIVEAPNFLSKINGIYNDKNEYIKFLDLLRDSESSTFIEF
                     NGDVDRFSKAFTREAFSLSFLLDSKGAIDKNSVLNNKDYSISTLALNQQIMILEKVEE
                     LLEGSMFLNGLSGEDKIKGLFTVLHMDKKFVHMMNNFDYSLINPKLIIYMYKSIVFDK
                     EIVFLMLLLSKIGFDIIILCPGGENNIENVINNQLIDVHRLDKMVYDLKLNSLENDIP
                     LLKKIFGKRRRF"
     gene            6644..7780
                     /locus_tag="PC414_002688"
     CDS             6644..7780
                     /locus_tag="PC414_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HDF2465076.1"
                     /translation="MGININSFSKDKNSVEVTENLPTAVQEENFDIMEYTNNKKNELR
                     KSKEVEALTSLIEVENPDTILQFGRKASEGVARVSDSLLNTIKLNRNEENSKMLVHLT
                     KIMDKFDLDDFQETKEPNFVQKLFKKANNAIEMMFQKYETLGGEVEKIQIELEQYERD
                     IALSNKQIGAMLNENFEFYNELQKYIVAGEMAIEEMDNEILPFFKQKSETSGDQMDVV
                     NYQELLKVYDMLNQRVYDLRIAENIAIQTIPMLRGMQHNNYGLIRKINSAFVVTLPVF
                     KQCLSQAILLKKQELQAKSLKALDDKTNELLLRNAQNVSTQSAQIARMAGTSSVQIET
                     LEKMYNTIKSGIDETMRIEENNRALIKDNTKRLEELNTTIIYNK"
     gene            7844..8920
                     /locus_tag="PC414_002689"
     CDS             7844..8920
                     /locus_tag="PC414_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HDF2465077.1"
                     /translation="MKYFDYICKDDLERIFLKEPEDFSAKTEKDVLKYALGAFLYVPA
                     TQYNMIYKSIIGDVKGVRPLAICLEDAVGVNGELEAIENLRLILKNISNESITNKDGI
                     PLIFVRIKDVEQLLRIKEIIIKNSHFITGILIPKANSELIENCIEALDFMNLQDMYVI
                     PIVETKEFIYNEKKELSFTNLYNAILRHKSRILSIRVGITDILGMYGIRRDKNFSIYN
                     NLICSSFILDIITYLQRPELDIPISGGVSEFFDMTNKDIRNKYIEEILLDKYHGLIGK
                     TVIHPMQIQIVQALSTVSYEDFTDALDILDSTDSKYGVSKGVLGERMNETNPHFLWAK
                     KTLILSKIYGVLNKGVDYEELLKF"
     gene            8898..10136
                     /locus_tag="PC414_002690"
     CDS             8898..10136
                     /locus_tag="PC414_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HDF2465078.1"
                     /translation="MKNCLNFNCEIDIIDNPLNLELNDFLDVGIRNNSKRRFLFISKK
                     LGKHLACKPSEMDNLGKLMATIYNEKNKEESNGTVIAFAETGTAVGHSFFDYLCGDYE
                     FIHTTREQFDELENLEFLEEHSHATDQNLYFGMLNNFKFGDEIILVDDEITTANTCMN
                     IIRKIQSIYPKKKYTICSILNWVSDENLSKVTRLERELDCKIDFVYLFKGNFKFYIDE
                     DIISNYNDNYSITKLETISSDKVNLVVNHIYVDLEDYIGNKKYVKYTGRFGINRREQF
                     RLKEIVKRESKKITMASKESLKNEKEKILFLGTEEFMYIPMLFAKEKENQYDIYYHST
                     TRSPIINIDKNNYPIKSKFMLKSFYNIDIQNYIYNIDKHNFSKCYLFVELNKSENSYL
                     EFIDIIKKTSIKELTIVCCS"
     gene            10190..11278
                     /locus_tag="PC414_002691"
     CDS             10190..11278
                     /locus_tag="PC414_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HDF2465079.1"
                     /translation="MRDFSTYSKDDVIFLLKDISNLIEEEGNKERELKIQNGRHYSEM
                     IPIEYEVSEDYLNLYHEKLRENKDKLAFSIGVMCEKIIKKNGNDVVLVSLARAGTPIG
                     ILAKRYIRSKYNISLPHYTISIIRDKGIDMNAIEYIVKNHPSSKIQFLDGWTGKGTIS
                     KELEKACDELNVKFKKYFDSTLAVLADPAGYSGLYGIREDFLIPSACLNSTVSGLVSR
                     TVLREDLIGKNDFHGAKFYRHLKDKDESMNYIETIEECFKNQFKNISDEVENWESDII
                     TRDGYFDVLNIKEKYNITDINFIKPGVGETTRVLLRRVPYKILVKDLNDKSLDHIFIL
                     AKEKNVEVEQMDLKAYKCCGIIKNMKDI"
     gene            11306..12097
                     /locus_tag="PC414_002692"
     CDS             11306..12097
                     /locus_tag="PC414_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HDF2465080.1"
                     /translation="MIVFSDLDRSIIYSNKFLNADSKYTNIEIYREKEISYISLDTIN
                     LIKQIQYYGMFIPTTTRTVEQFKRIEFNKYGIYFPWSITSNGGVILKDNEILKSWSEK
                     IDKLKCDYEPIESMIHKFKDYLNVDGITNFKVAEDTFFYIVVDLSRFNLDSIKEYTNI
                     LESKNWKFYVSGRKIYFIPKEISKENAIKYLTKELGIEYFYAVGDSIMDYGMLNISNK
                     SYVLKHGDINKNEIENSFISSSFNGMSGTEEILSNILDENCLLNI"
     gene            complement(12212..12766)
                     /locus_tag="PC414_002693"
     CDS             complement(12212..12766)
                     /locus_tag="PC414_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDF2465081.1"
                     /translation="MNIDVGSKIRSLRKSKNISISTLAKNSDLSTGLISQIERNMVVP
                     SIVAMWKISKALDINIGYFFEEIGREDSDIVVKKNNRKKIVTNDSTKFYELLVPNLSG
                     KKIEFILVTLDGHTQKNSEFVTHEGEECGYIIKGKMKITLGNKEYLLEEGDSFYFNST
                     IPHVYENYDDEVCISVWAMTPPSF"
     gene            12962..14164
                     /locus_tag="PC414_002694"
     CDS             12962..14164
                     /locus_tag="PC414_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 domain-containing protein"
                     /protein_id="HDF2465082.1"
                     /translation="MKSFVSPDNIWVLWAVVTGWAAFSIYLEQKYNWASKVSGAIIAL
                     VGAMLLSNLNIIPVESVVYDQVWGYVVPLAIALLLYQCNIKKIWKESGRLLIIFLVGS
                     VGTVLGAMIGFLALKNVVPDLNIVAAMMTGSYIGGNVNFAAMSGAFDAPGELVSATVV
                     ADNLLMALYFFVLIAIPSIGFFRKHFKHPHVDEMESIGITEGETVAANYWGRKEISLK
                     DIAIAIGSAFIIVAVSVELSTWFKAIIPLSNPFLAMLNTLFGNQYLIITTLTMLFATF
                     MPNFFGDIKGAQELGTFLIYIFFVVVGVPASITLIIQKSPLLLLYCGIMVLINMLVTF
                     IVAKIFKFSLEEAILASNANIGGPTTAAAMAISKGWSKLVGPILIVGTFGYIVGNYFG
                     LLVGNILI"
     gene            14221..15201
                     /locus_tag="PC414_002695"
     CDS             14221..15201
                     /locus_tag="PC414_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HDF2465083.1"
                     /translation="MIFDGHSDTWADVTIRRLNGEKDVFRRIHLDNFKRANIEGAIFV
                     IWIDPPYDKEPEKRVKQIISSIKDEIEDMKDVVNIVKKYSDIDKGISENKINILIGLE
                     GLSHIGRNIDLLDYFYKEVGARHASLTWNEQNELATGCLGDENRGLSEIGKKAVNKME
                     KLGMIVDVSHLNEKSFWDLMSVASKPVIASHSNARSLCDAKRNLTDNQLKEISNTGGL
                     VGINSFRGFTNSNPKKQDLIHLVDHIDYMVNLIGIEHVSFGFDFSDYLGKETIGSFTG
                     NASVPSVDGLESTHTAQNIVVELENRGYKKKDIELISSGNFYRLFKDILK"
     gene            15444..16397
                     /locus_tag="PC414_002696"
     CDS             15444..16397
                     /locus_tag="PC414_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDF2465084.1"
                     /translation="MKKRYLVIGIIILSYFSLFIGAEDINIMHIFAKNQHKLMIFIMS
                     RIPRLISILIAGVGMSIAGLIMQQISKNKFVSPTTGATIDAAQFGIVICMLLVPTASI
                     FTKTIIAFVFSLVGTFTFMKIIGKLQFKNIIFVPLVGIMFGNIIGSMTDFIAYKYDLS
                     QNVSSWMQGDFSMILKGNYEILYITIPLIILAYIYANKFTVVGMGMDFATNLGLSYKR
                     IVNIGLIIVALVTVCVVVTAGNIPFIGLIVPNIVSLYMGDNIRASIWYTGLLGAIFVL
                     ICDIFGRIIIYPYEISIGLTVGVIGSILFLYLILRRNVNEA"
     gene            16387..17343
                     /locus_tag="PC414_002697"
     CDS             16387..17343
                     /locus_tag="PC414_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDF2465085.1"
                     /translation="MKLSLNQKIYFVSILLFISMALFIIYGIDMNHLEYALSQRIPKI
                     FAMVLGGGCIAFTTVVFQTITNNQILTPSVLGLDSLYVMIQTIIVFIFGSSSSLIINE
                     NYNFIINVAMMIGASLLLYKTLFEKNKNNIFFLILVGMIFGTLFKSATTFIQVMIDPN
                     EFLALQTSIMASLNNINTNVLLIAFIIIIAIIPFIYDEIKYLDVLSLGKEQAINLGVD
                     FDKAVKKMIILIAILVSISTALIGPMTFLGLLLANITREIFKTYKHTYLISGSMLIGM
                     ITLIIGQFFIQHVFKFDTTLSVVINFIGGIYFIQLLLKGANR"
     gene            17340..18095
                     /locus_tag="PC414_002698"
     CDS             17340..18095
                     /locus_tag="PC414_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2465086.1"
                     /translation="MIEIKNIFKRYKNKNVVDDVSFSIEKGKITSFIGPNGAGKSTVL
                     SIVTRLIGGDGGEVIIEGKSLTNYSNKELAKKIAILKQSNNITLKLTIRELVGFGRFP
                     YSEGNLTKEDENYIDEAIEYMKLTDIQHKYLDELSGGQRQRAYIAMVIAQDTEYILLD
                     EPLNNLDMNHSVQMMKVLRGLCDELDKTIVLVMHDINFASCYSDNIVALKNGKVEKVG
                     RTDEIVNEKVLEDIYEMNFNIKNINGNRICIYF"
     gene            18187..19152
                     /locus_tag="PC414_002699"
     CDS             18187..19152
                     /locus_tag="PC414_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2465087.1"
                     /translation="MNKKAAIVAAVAIIGLVTVFALGGSKKNESKTSENSNNTMKITH
                     NLGETDVKLNPKKVVVFDYSALDTMDALGVAETLVGLPKASLPASLEKYKDDKYADLG
                     GLKEPDLEGIKSANPDLIIINGRQEDFYEQLSKIAPTISTSKDDKKYLESVKNNIDKI
                     AKIFGVEEKANQEFSKIEKKIETLNKKVTDKNLNALTIMVNEGNLSVFGEESRFSILY
                     NSFGFENKDKNIKESSHGQNITFEYIAKQNPEVMFVIDRGIATGSDVKESSTAKSVLN
                     NDIIKSMDAYKNDNIIYLDSPTWYVNDGGLTSLNKMIDDASKAVN"
BASE COUNT         7649 a         1970 c         3457 g         6263 t
ORIGIN      
        1 ggtagtagtg gcgaagacac cgtccgaagt agcttggctt tgtaaccacc tctgttgtac
       61 tgtttaagta taaataagtg tgttagccac caagaatccc cttgctttag ctatggggag
      121 tgtgtcaaat attaaacata gcttttatac atttaataag gtatgtaaaa tctatgtaaa
      181 aagtaagaaa tataaacatt tttttaatta tattttaaaa atatcaagca agttttcttc
      241 acaaatgaat aaaaaatgtt ataatatgat taaagaaaaa gataaataat tagcaagatt
      301 tgtttagcta aaattaacaa gggagcgata aattatgggt ataacattat caaaaggcca
      361 aaaggttgat ttaactaaag gaaatccagg tcttaaaaat attttagtag gtttaggatg
      421 ggatactaac aagtatgatg gtggatttga ctttgattta gatacagcag catttttaac
      481 aggagcaagt ggaagtgtta ctaatgatgg agattttgta ttttacaaca atttaaaaca
      541 tacatctgga gcagttgagc atctaggaga taacagaact ggcgaaggtg atggagatga
      601 tgaacaaata gttgttgatt tatctaagat tcctggagaa ataagcaaga tttcttttac
      661 tgttacaatt catgatgcaa cagaaagaag acaaaatttt ggacaagtaa gtaattctta
      721 tataagaata gtagataaag acacaaatga agagttaata aagtatgaat taggagaaga
      781 ttttagtata gaaacagcta tagtagtagc tgaaatatat aagcacaatg gagaatggaa
      841 gtttaatgca ctaggttctg gctttgaagg tggtcttgct gcattatgtg gaaactttgg
      901 aataaactta taatttcaac tataacaata aactaaaggg ggatattttc catggctata
      961 gtattaaaaa aaggacaaaa aatcgattta actaagggaa atccaggtct taaaaatata
     1021 aaacttggac taggatggga tacaaattca tttgatagtg gctatgatta tgatttagat
     1081 gtaagtgttt tcatggttgg agaatctcaa agagttgaaa aagatgaaga ttttatattc
     1141 tacaataact taaaacatcc atctggagca gttgaacact taggtgataa tagaactggt
     1201 gaaggtgatg gagatgatga ggaaatatta gtgaatttag agacaatgcc aaagcatata
     1261 caaaagatag ctgtaacagt tacaatatat gaggctgctg aaagaagaca aaattttgga
     1321 caagtaagta actcatatat aagagtatta aattctgaaa atgatgaaga aatattaaga
     1381 tatgacttag gtgaagaatt tagtatagaa actgcaatta cagtatgtga gatatataag
     1441 tataatggag aatggaagtt tagtgcagtt ggagcaggat ttgaaggtgg cttagaagca
     1501 ctttgtagaa actatggctt aaatgtttaa tttaagcaat ataatatatt aaatataaat
     1561 aacctataca aataatgtat aggttatatt atataaaatt ttgtggaggt ttagtatgtc
     1621 gattaattta tcaaaaggtg ataaaataga tttaaaaaaa tctaacccag gactgagtaa
     1681 catacttgta ggtctaggat gggaccctgt gcaacaatct ggtggaggtt tttttaaatc
     1741 gttatttggt ggaggacaag ctgatataga ttgtgatgca tctgtattta tgcttaatca
     1801 agaaggtaaa ttaagtggaa taaaagattt aatttatttt ggaaatctaa aaagtgcctg
     1861 taaatcagtt ttgcatacag gagacaatct aactggtgag ggtacaggtg atgatgaaca
     1921 aatcctagtt aatttggata aagttccatc aaatattcat aaattattgt ttgtagtaaa
     1981 tatatacaat tgtgtagata gaaaacaaca ctttggaatg atagaaaatg cctatataag
     2041 agttgaagac caaggtaata agaaagagat agcaaaatac aatctatctg ataattattc
     2101 tgaaaaaact actcttatag taggagctat atatagaaaa gatggaagtt ggcaatttaa
     2161 agcaattggt gaaggaacaa aagatgcggg tcttaaagaa gtaatgcaaa atttagacag
     2221 gattgagtgt gcatatggaa tttaataatg atatcaaata ttataatgta gataaagaag
     2281 aagttacaaa agacttatct gttaatcctg aaaaaggttt atcagaatca gaagtcaaga
     2341 ctagaagaga aaaatatggt ttgaatgaat ttactccaaa agaagagggt agtttttggg
     2401 atgatttaaa ggaaagttta agtgaaccaa tgatagttat cttgattatt gcagcagtag
     2461 taagtgcagt aattggggaa actcatgatg caatagggat tgtaggtgct atagcaatag
     2521 gtatagcaat aggtatgatt acagaaggtc gttctaagaa agcagcagag gctctttcta
     2581 agttgactga aaacatagaa gtaaaagtat tgagagatgg ggaagtacat caaatttcta
     2641 agagtgaatt ggtaccaggt gatatagtat atattgaaac tggtgacatg gtacctgctg
     2701 atggtagact tatagaaagt ataaacctta aaattagaga agatatgtta acaggagaat
     2761 ctgatgacgt atctaaaaag tgtgatgtag tagtttctat ggaagagata gaatctaaag
     2821 gagcagtagt agtacaggag ccaatacctg caaaacagat aaatatggtg tttggtggca
     2881 cacttgtagc ttatggtaga ggagctctag ttgtaacttc aattggagat aatagtgaaa
     2941 tgggtaaaat tgctcaaaac ttatcagaaa cagatgagca aactccactt cagataaaac
     3001 ttggaaattt aggtggtttg atagccaaag tttctagtgc agtagcagga ttactattta
     3061 tatttatggt tttccaaatg ataagtaaga atgtattaaa tgtagatatg tctggaatat
     3121 taccgttttt agaatcaata gacccagtta aaacagcatt tacagtatgt gttgcgttga
     3181 tagttgcagc agtgccagaa ggtcttccaa ctatgataaa tatgacactt gcaataacaa
     3241 tgcaaaaaat ggctaaaata aatgcattgg ttactaaaaa agaagcttgt gaaacgatag
     3301 gttctgtttc tgtaatatgt tcagataaaa ctggtacatt aactgaaaat agaatgactg
     3361 tagaagttgc atatgttgat ggcagatata ttgaaagttc agaagaggaa ataaatagtt
     3421 attttgaaga aaactgtatg ataaactcta cagctgatgt agagcacaat gatggtgata
     3481 ttaagtattt aggaagtgct actgaatgtg cattgctttt atattacaaa aacgtagatt
     3541 atagacaagt tagaaaaaat tctaatatag tagctcaaaa tccttttact tcagattcaa
     3601 agagaatgac ttctgttata ggtcaagata atcatcatgt actattatca aaaggagcac
     3661 cagaagtctt acttgaatta tgttcacata ttcaaagagg aaaagacata gttccattaa
     3721 ctgaaaatat aaaacatgag attttagaag aaataaagaa attgcaaata aaatcaatga
     3781 gaactttagg gtttgcatat aaagaaatct cacaagctga agaagaagca gcagtaactg
     3841 ctgaaagtga tgctatgaat gtaagtgcaa tggaaaacaa tttagtattt agtggttttg
     3901 ttggtataag agacccactt agaaaagatg ttatagagtc tgtaaataca gcaaataaag
     3961 ctggtgtttc tgtaaaaatg cttacaggag ataatataaa tacggcaaga gctataggtg
     4021 aagagttagg cctattaaaa aataatatga gagtggttga agcttcatat atagatactt
     4081 tagatgatga agaattaaaa caagagatac aaagtatttc aatcgtggca agaagtaaac
     4141 cagatagtaa aatgagaata gtttcagctc tgcaaaagag cggagatgtt gttgcagtaa
     4201 caggtgatgg tataaatgat gctccagcac tttcaaaagc agatgttggt atagcaatgg
     4261 gtatatctgg tacagaggta tctaaaaacg cagcagacat aattttaact gatgatagtt
     4321 ttagtaccat tgtaaaaggt attaaatggg gtagaggaat ttatgataac ttccaaagat
     4381 tcgttcaatt ccaattaact gtaaatgtaa ttgcattttt agtggctata atatctcaag
     4441 ttatgggaca agagatgcca tttactacaa tacaattatt atgggtaaac atcattatgg
     4501 atggaccacc agcactagca ttaggtttag agccagttag ggaccatgtg ttaaatagaa
     4561 aacctgttga tagaaaagcc aatataattt ctaagtcaat ggtgtataca atagtattaa
     4621 atgcatttta tataacagca atactaatgt tacaatctac atttaatttc ttaggagttg
     4681 atgaagcgca taagggaaca gtaatgtttg cgttatttgc ttttagtgca ctgtttaatg
     4741 catttaactg tagagagttt aatgcagata gtatcttccc taattttaca cataacaaat
     4801 tagcactaca aataatagtg ttgacaggaa ttgcacaagt aatatttact cagctattcc
     4861 aagatttctt taactctgta gcaatggatt ttatgacatg ggtaaaagtt ttatgtgtag
     4921 cagcaagtgt aattgtggtt aatgaagttg taaaatgtat aataagactg gtaaaactaa
     4981 aaaagactgc ttaaaacaaa ttttttagaa gaaaacagac tgactcaaaa tatgaatttt
     5041 gggtcagttc tgaataaaca tttaaattgg gaggatagtt atgggtgact caaaggtatt
     5101 tgaaaaaata ttctccagcc aatcagatag aggaaattac actccttcaa agggatatct
     5161 aagttatttt atttcgtata ttggtttaga agatgaagtt ttgtacaatc tcgagatatt
     5221 taagacaaaa caaaatattg atagcaaaaa agatattgct ttatttactg atgttatagc
     5281 aaatcctagt gattttgata ttataaatta ttttaaaagt ggacttcaaa aatatagaac
     5341 tagcatggaa gatgtagata taaatatttt gggatttgaa gagattgatt ataaaataaa
     5401 gcaagcaatg gatagagtgt taaaagaaga ggaaaaagaa tttactaatg atagagttaa
     5461 acaaaatttt atagtgaaaa taatggcatg gataaaaatt tatatcggtg ctttagatat
     5521 aaataaaaat gaagctccaa aagtaatatt ttatggagat ataaaaaaac atgaagttta
     5581 tttactctta atattatact tggcaggttt tgatgtttta tatcttaatc caaattcaaa
     5641 gagtaatata gatattttaa aatcagaaag atacaatata gagtttgaag aagccaacat
     5701 aatagaagaa aaaatttctt ttgaagaaag ggttatttta ggggaaaaaa tagataaaag
     5761 ttctgtaaaa aaagctttta ctgttggggc agaagcatca aagcgtataa gtgaggaatt
     5821 gttaaatgat gcaggattta taaaaccttg gcaactccaa gatagaaaaa taaaaaatct
     5881 acttctaagt tcaactgttg atgaaatatc aatatactgg aatcaaccac ttaaattaag
     5941 accaggattt aagtttaatg atgctattgt agaagctcca aattttcttt ctaagattaa
     6001 tggaatatat aacgataaga atgaatatat taagttttta gatttattaa gagattcgga
     6061 aagttcaaca tttattgaat ttaatggaga tgtagataga ttttcaaaag cttttacaag
     6121 agaagctttt agtttatcct ttttattgga ttcaaaaggt gcaatagata aaaactctgt
     6181 tttaaataat aaagattatt ccatatcaac actagcattg aatcaacaaa ttatgatttt
     6241 agagaaagtg gaagagttgt tagaaggaag tatgtttttg aatggtttaa gtggagaaga
     6301 caagataaaa ggtctattta cagtacttca catggataag aaatttgttc atatgatgaa
     6361 caatttcgat tactcactta taaacccaaa attgattata tacatgtaca agagtattgt
     6421 atttgataaa gaaatagttt ttcttatgct tttacttagt aagattggat ttgatattat
     6481 catattatgt ccaggaggag aaaacaatat agagaatgtt ataaataacc aattgattga
     6541 tgtacacaga ttggataaaa tggtatatga ccttaaatta aattcgcttg aaaatgatat
     6601 acctttactt aaaaaaattt ttggaaagag gagacgattt tagatgggga taaatataaa
     6661 tagtttttca aaagataaga actctgtaga ggttactgaa aatttgccta cagcagttca
     6721 agaagaaaac ttcgatataa tggaatatac aaataataaa aaaaatgaac tgagaaagtc
     6781 taaggaagtt gaggcattga catctcttat agaagtagaa aatccagata cgattttaca
     6841 gtttggtagg aaagcatctg aaggagtagc aagagtttct gatagccttt taaatacaat
     6901 aaaattaaat agaaatgaag aaaattctaa aatgttggtt catttaacta aaataatgga
     6961 caagtttgat ttagacgatt ttcaagaaac taaggaacca aatttcgttc aaaaactttt
     7021 caaaaaagct aataatgcca ttgaaatgat gtttcaaaag tatgaaacat tgggaggaga
     7081 agttgagaaa attcaaattg agcttgaaca atacgaaaga gatatagcac tttctaataa
     7141 gcaaataggt gctatgttaa atgaaaactt tgagttttac aatgaacttc aaaaatatat
     7201 tgttgctgga gaaatggcaa tagaagaaat ggataatgaa atactacctt tctttaaaca
     7261 aaaatcagag actagtggag accaaatgga tgttgtcaat tatcaagaac tattaaaagt
     7321 ttatgatatg ttaaatcaaa gagtctatga tttaaggata gcagaaaata tagctatcca
     7381 aacaatacct atgcttagag gtatgcaaca taataattat ggtttaatta gaaaaataaa
     7441 tagtgcattt gtcgtaactt taccagtatt taaacaatgt ttatctcaag ctatattact
     7501 taaaaagcaa gaattacaag caaaatctct aaaagcactt gatgacaaaa ctaatgaatt
     7561 attgcttaga aatgcacaaa atgtaagtac tcaaagtgca caaatagcta gaatggctgg
     7621 aacaagttct gttcaaatag aaacattgga aaaaatgtac aatactatca agtctggtat
     7681 agatgaaact atgagaatag aagaaaacaa tagagcttta attaaggata atacaaagag
     7741 attggaagaa ttaaatacga ctataattta taataagtag tattatatta agggctaatt
     7801 tgtataaagt tggccctttc tatgtatatg aggaggctag acattgaaat atttcgatta
     7861 tatctgcaaa gatgatttgg agagaatttt tttgaaggag ccagaagatt ttagtgctaa
     7921 gactgaaaaa gatgtgttga aatatgcact gggcgctttt ttatacgttc cagctactca
     7981 atataatatg atttataaga gtattatagg ggatgttaag ggagttagac cattagctat
     8041 ttgtttagaa gatgctgttg gagtaaatgg agagttggag gctatagaaa atcttagatt
     8101 aattttaaaa aatattagca atgaaagtat aactaataaa gatggaatac cactaatttt
     8161 tgtaagaata aaagatgtgg agcaactgtt gagaattaaa gagattataa ttaaaaatag
     8221 tcattttata acaggaatat taattcctaa agcaaattcg gagttaattg aaaattgtat
     8281 agaagcttta gattttatga atcttcaaga tatgtatgtt attccgatag tagagacaaa
     8341 agaatttata tataatgaaa aaaaagaact tagttttact aatttgtaca atgctatttt
     8401 aagacataag tcaagaatat taagcattag agtaggcata actgatatat tagggatgta
     8461 tggtataagg agagataaaa acttttctat atataataat ttgatttgtt cttcatttat
     8521 attagatata atcacatatc ttcaaagacc agagttagat atacctataa gtggtggagt
     8581 atcagaattt tttgatatga cgaataagga tataagaaat aagtatatag aagaaatatt
     8641 attggataaa tatcatggat taattggaaa gaccgtaatt catccaatgc aaattcaaat
     8701 tgtacaagcg ctatctacag tatcatatga agattttact gatgctttgg atatactgga
     8761 tagtacagac agtaagtatg gtgtgagcaa gggtgtatta ggagagagaa tgaatgaaac
     8821 aaatccacat tttttatggg ctaaaaaaac attgattcta tctaaaatat atggtgtatt
     8881 aaataaagga gtagactatg aagaattgct taaattttaa ttgtgagatt gacattattg
     8941 ataatccatt gaatttagaa ctaaatgatt ttttagacgt aggaataaga aataatagca
     9001 aaagacgttt tttgtttatc tcaaagaaac tcggtaaaca tttagcatgt aagccaagtg
     9061 agatggataa tttgggaaaa ttaatggcta caatttataa tgaaaaaaat aaggaagaat
     9121 caaatggtac tgttatagct tttgctgaaa ctggtacagc tgttgggcat agtttttttg
     9181 attacttatg tggagattat gaatttattc atactactag agagcagttt gacgagttag
     9241 aaaatctgga atttttagag gaacattccc atgcaacaga ccagaattta tactttggta
     9301 tgcttaacaa ctttaaattt ggagatgaga taatattggt tgatgatgag attaccacag
     9361 ccaatacatg catgaatata attaggaaaa ttcaaagcat ttatcctaaa aagaaataca
     9421 ctatatgctc aatactcaat tgggtaagtg atgaaaattt aagtaaagtt actcgtttag
     9481 aaagagagct agattgtaag atagattttg tatacttatt taaaggaaac tttaaatttt
     9541 atatagatga agatattatt tcaaattaca acgataatta tagtattact aaattagaga
     9601 ctatttcaag tgataaagta aatttagtag taaatcatat ttatgttgat ttagaagatt
     9661 atattggaaa taagaaatat gtaaaatata caggaagatt tggaattaat agaagagaac
     9721 aatttagatt aaaagaaatc gttaagagag aaagtaaaaa aataactatg gccagtaaag
     9781 aatctttaaa aaatgaaaaa gaaaagattc tatttttagg tactgaagaa tttatgtata
     9841 tacctatgct gtttgcaaaa gaaaaagaaa atcaatatga tatatactat cattcaacaa
     9901 caagaagtcc aattattaat attgataaga ataattatcc tattaaaagt aaatttatgc
     9961 taaagagttt ttataatata gatattcaaa attatattta caatatagat aaacataatt
    10021 tttctaagtg ttatttattt gtagagctga ataaaagtga aaattcttat ctagaattta
    10081 ttgatataat taaaaaaaca agtataaaag aattaactat agtatgttgt agctaaagtg
    10141 ttttaacagg ataaaatata tcacgatttt aagataggag ttgagtcata tgagagattt
    10201 tagcacgtat tctaaagatg atgttatttt tttattaaaa gatatatcaa atttaattga
    10261 agaagaagga aataaagaaa gagaattaaa aatacaaaat ggtagacatt attcagaaat
    10321 gataccaata gagtatgaag ttagtgaaga ttatctgaat ttatatcatg agaaactaag
    10381 agaaaataaa gataaacttg cattttctat aggagttatg tgtgaaaaaa taattaaaaa
    10441 aaatggaaat gatgtagtac tagtttcact tgctagagca ggaacaccta ttggtatttt
    10501 ggcaaaaaga tatataagaa gcaagtataa tataagttta cctcactata caatctctat
    10561 aataagagat aagggaatag atatgaatgc aattgaatat atagtaaaga atcatccgag
    10621 ctcaaaaatt caatttttag atggatggac tggtaaggga actatatcaa aagaacttga
    10681 aaaagcatgt gatgaactta atgttaaatt taaaaaatat tttgactcaa cacttgctgt
    10741 attagctgac cctgctggat attcaggact ttatggaatt agagaagatt tcttaatacc
    10801 aagtgcatgt ttaaattcaa cagtttctgg tttagtaagt agaactgtac taagagagga
    10861 tttaattggt aaaaatgact tccatggagc taagttttat agacatttaa aagataaaga
    10921 tgaatctatg aactatatcg aaactataga agaatgtttt aaaaatcaat ttaagaatat
    10981 atctgatgaa gttgaaaact gggaaagtga cattataaca agagatggat actttgatgt
    11041 tttaaatata aaagaaaagt ataacataac agatattaac tttatcaaac caggtgtagg
    11101 ggaaacgaca agggttttac ttagaagagt tccgtataag attcttgtta aagacttaaa
    11161 tgataaatct ttagaccata tattcatatt agctaaggag aaaaatgttg aagttgaaca
    11221 aatggattta aaagcctata aatgttgtgg aataataaaa aatatgaaag atatttagga
    11281 aatatattag acaggagaat attgaatgat tgttttttca gatttagaca ggagtatcat
    11341 ttactccaat aaatttttaa atgctgatag taaatatact aatatagaaa tttataggga
    11401 aaaagaaata tcttatatat ctttagatac tataaattta ataaagcaaa tacaatatta
    11461 tggtatgttt atacctacaa ctactagaac agtggagcaa tttaagcgta tagaatttaa
    11521 taaatatggg atttattttc cttggagtat aacttcaaat ggaggagtta tacttaaaga
    11581 taatgaaata ttgaaatcat ggagtgaaaa aatagataaa ctaaaatgtg attatgagcc
    11641 cattgaaagt atgattcata aatttaaaga ctatttgaat gtagatggaa taactaattt
    11701 taaagttgca gaagatacat ttttttatat agttgttgat ttatcaagat ttaatttgga
    11761 ttcaatcaaa gaatatacaa atatattgga atcaaaaaat tggaagtttt atgtgagtgg
    11821 cagaaaaata tattttatac caaaagaaat ttcaaaagaa aatgctataa aatacttgac
    11881 taaagaatta ggaatagaat atttttatgc agttggagat tctattatgg actatggtat
    11941 gcttaatatc tcaaataaat cctatgtatt gaagcatggt gatataaata aaaatgaaat
    12001 agaaaattca tttatatcaa gttctttcaa tggaatgagt ggaactgaag agatactatc
    12061 taacatttta gacgagaact gcctacttaa tatataatga ctgatatata aataataaac
    12121 tagaaaaatc tgaaataaat tatagtaaag tttatcatac taatttattt cagattttta
    12181 tcaatttata tttaagtaag aactcaatat gttaaaatga aggtggagtc attgcccaga
    12241 ctgagataca tacctcatca tcataatttt catacacatg tggtatagta ctgttaaaat
    12301 aaaaactatc tccttcctcc aatagatatt ccttatttcc aagtgttatc ttcattttac
    12361 cttttattat atagccacat tcttctcctt cgtgtgtcac aaattcactg tttttttgtg
    12421 tatgaccatc tagtgtaaca agtataaatt ctattttttt cccagataaa ttaggaacaa
    12481 gaagttcata aaattttgta gaatcattag ttactatttt ttttcgatta tttttcttta
    12541 ctactatatc agagtcttct cttccaatct cttcaaaaaa ataacctata tttatatcaa
    12601 gagcttttga tattttccac attgctacaa tagatggaac aaccatatta cgctcaattt
    12661 gacttattaa gcctgtactt aaatctgaat tttttgctaa agtagagata cttatatttt
    12721 tagattttct aagagatcta attttgcttc caacatctat attcattaga tacaaacccc
    12781 ctcagacatg attataacat ataatttata aagattaata aaataaaaaa aaagttttac
    12841 aatacaagta ttttttatgc tatattgaat tttctgaaaa gatgcattat aatagtatta
    12901 atattcaata tattgaatat tgataacgat attttaattt aatgaaatgg aggtaattaa
    12961 gatgaagagt tttgtaagtc ctgataatat ttgggtatta tgggcagttg taactggatg
    13021 ggcagcattt agtatttatt tggaacaaaa gtacaattgg gcatcaaaag tgtcgggagc
    13081 aataatagct cttgtaggag caatgttatt atcaaatcta aatattatac cagtagagtc
    13141 tgttgtatat gaccaagtat ggggatatgt tgttccttta gccatagctt tattgttata
    13201 tcaatgtaac attaaaaaga tatggaaaga aagtggaaga ttgctcatta tatttcttgt
    13261 aggttctgta ggaactgtac ttggagctat gattggattt ttagcattaa aaaatgtagt
    13321 accagattta aatattgttg cagctatgat gacaggttct tatataggtg gaaatgtaaa
    13381 ctttgctgct atgtcagggg cgtttgatgc gccaggagaa ttagtatcag caacagttgt
    13441 agcagataat cttttaatgg cattatattt ctttgtctta atagctatac catcaattgg
    13501 attttttaga aaacatttta aacatccaca tgtagatgaa atggaaagta taggtataac
    13561 tgaaggagaa acagtagcag caaattattg gggaagaaaa gaaatatctt taaaagatat
    13621 agcaatagcc attggtagcg cctttataat tgttgcagta tctgtagaac tatcaacttg
    13681 gtttaaagct ataataccat tatcaaatcc ttttttagca atgttaaata ctttatttgg
    13741 aaatcaatat ctaataataa ctactttgac tatgttgttt gccacattta tgcctaattt
    13801 ctttggtgat ataaaaggag cacaggaatt gggtacattt ttaatatata tattctttgt
    13861 tgtagtaggt gttcctgctt caataacatt aataattcaa aaatcaccat tactactttt
    13921 atactgcgga ataatggtac taataaacat gctagttaca tttatagttg caaaaatatt
    13981 taagtttagt ttagaagaag ctattcttgc atctaatgca aatattggtg gtcctacaac
    14041 agcagcagct atggcaatat caaaaggatg gtctaagcta gtagggccaa tacttatagt
    14101 tggaacattt ggatacatag tcggtaatta ttttggtctt ttagtaggaa atattttgat
    14161 ataattttac gtgaaaaaaa gttgtttatt agaaagattt ttatttaatt ggaggttgtt
    14221 atgattttcg atggacattc agatacttgg gcagatgtaa caataagaag gcttaacggg
    14281 gaaaaagatg tatttagaag aatccatctt gataatttta aaagagcaaa tatagaagga
    14341 gctatttttg taatatggat agacccacca tatgataaag agcctgaaaa aagagttaaa
    14401 caaattataa gttctataaa agatgaaatt gaagatatga aagatgttgt aaacatagta
    14461 aaaaaatatt cagatataga taaaggtata agtgaaaaca aaataaatat tttaattggt
    14521 cttgaaggat taagccatat tggaagaaat atagatttac tagattattt ttataaagaa
    14581 gttggtgcaa gacatgcatc attaacttgg aatgaacaga atgaacttgc tacaggatgt
    14641 ttaggggatg aaaatcgtgg tttaagtgaa ataggcaaaa aagcagtcaa taaaatggaa
    14701 aaattgggca tgatagtaga tgtgtcacat ttaaatgaaa aatcattttg ggatttaatg
    14761 tctgttgcaa gtaagccagt tatagcttct cattctaatg ctaggagttt atgtgatgct
    14821 aaaagaaatc ttacagataa tcaattaaaa gaaatttcaa atacaggtgg attagttgga
    14881 ataaatagtt ttagaggatt tactaatagt aatcctaaga agcaagattt aatacatttg
    14941 gttgaccata tagactatat ggtaaatctt ataggtatag aacatgtatc ttttggtttt
    15001 gattttagtg attacttagg gaaagagact ataggttctt ttactggaaa tgctagtgtg
    15061 ccaagtgtag atggtcttga aagcacacat acagcacaga acatagtagt agagcttgaa
    15121 aatagaggtt ataaaaagaa agatatagag ttaatctcta gtggtaattt ttataggctc
    15181 tttaaagata tactaaaata atataataaa gaacttaaat ttataaaata gtatatttat
    15241 acaaataata tctacatatt taaattggcg ttcataattt attaaatttt aatgttacta
    15301 gaagtttcaa ttctagtaac attttttatg ttaaaaacag atatagatat gtattgacaa
    15361 tgataattat tattgataaa ctataaaagt gaaaatgata ataattttca ttttaattaa
    15421 taaaagattt ttaaggaggg agaatgaaga aaaggtattt agtcatagga ataataattt
    15481 tgtcttattt ttctttattt ataggagctg aagatataaa tataatgcat atatttgcca
    15541 aaaatcagca taaacttatg atatttatca tgagtagaat accaagatta ataagtatat
    15601 tgattgctgg tgtaggaatg agcatagcag gtcttataat gcaacagata agtaagaata
    15661 aatttgtatc acctacaaca ggtgctacaa tagatgcagc acaatttggg atagtaatat
    15721 gtatgctttt agtaccaact gcctctattt ttacaaagac aataatagca tttgtatttt
    15781 ctcttgtagg gacttttacg tttatgaaaa taatagggaa gttacaattt aaaaatataa
    15841 tctttgtacc attagtaggt ataatgtttg gaaatataat agggtctatg acggatttta
    15901 tcgcgtataa atacgatttg agtcaaaatg ttagttcttg gatgcaaggt gacttttcta
    15961 tgatacttaa aggaaattat gagatattat atatcacaat accccttata atattagcat
    16021 atatttatgc aaataaattt actgttgttg gtatgggtat ggattttgcg acaaacttag
    16081 gtcttagtta caaaagaatt gtcaatatag gtctgataat agtagcactt gttacagtat
    16141 gtgtagtcgt tacagctggt aatatccctt ttataggtct aatagttcca aatatcgtat
    16201 ccttgtacat gggtgataat ataagagcaa gcatatggta tactggttta cttggagcga
    16261 tatttgtact tatatgtgat atttttggaa gaattattat ttatccatat gaaatatcta
    16321 tagggcttac agttggagtt ataggaagta tactattcct ttatttaata ttaaggagga
    16381 atgtgaatga agcttagtct caatcaaaaa atttactttg taagtatatt actattcata
    16441 tcaatggcac tttttataat ttatggaata gacatgaatc atttagaata tgcattatca
    16501 caaagaatac caaaaatatt tgctatggta ttaggtggag gatgtatagc atttactact
    16561 gtagtgtttc aaacaattac aaacaatcaa atattaacac caagtgtact aggtttagat
    16621 tcattgtatg taatgattca aactataata gtattcatat ttggttcaag cagtagtctg
    16681 attataaatg aaaattataa ttttattatt aatgtagcta tgatgattgg agcttcattg
    16741 ttattatata agacgttatt tgaaaaaaat aaaaataata ttttcttttt aatcctagta
    16801 ggaatgatat ttggaacctt atttaaaagt gcaacaacat ttattcaggt aatgatagac
    16861 cctaatgaat ttttagcttt acaaactagt ataatggcaa gtttaaacaa tataaacact
    16921 aatgtattgt taatagcatt tataataatt attgccataa taccttttat atatgatgaa
    16981 ataaagtatt tagatgtatt gtcgttaggt aaagaacaag caattaattt aggtgtagat
    17041 tttgataaag cagttaaaaa aatgataata ttaatagcaa ttttagtctc aatatcaaca
    17101 gcacttattg gacctatgac atttttagga cttttacttg ctaatataac aagagaaatt
    17161 tttaaaacat ataagcatac atacttgatt tcgggttcta tgttgatagg gatgattaca
    17221 ttgataatag gtcaattttt tatacaacat gtatttaagt ttgatacaac tttaagtgta
    17281 gtaataaatt ttataggagg aatttatttt attcaattac tattaaaggg ggcaaataga
    17341 tgatagagat aaaaaatata tttaaaagat ataagaataa aaatgttgta gatgatgtct
    17401 cttttagtat agaaaaggga aagataacat cttttatagg accaaatgga gctggaaaaa
    17461 gtacagtact atcaatagtt acaagactta taggaggtga tggtggagaa gtaataatag
    17521 aaggaaaaag cttgactaat tatagcaata aagaattagc aaaaaaaata gctattctta
    17581 aacaatctaa caatataact ttaaagctta caataagaga attagttgga tttggaagat
    17641 ttccatatag tgaaggtaat ctaactaaag aggatgaaaa ttatattgat gaagcaatag
    17701 aatatatgaa gttgactgat atacaacata agtatttaga tgaattgagt ggaggtcaaa
    17761 gacaaagagc ttatatagca atggtaatag ctcaagatac agagtacata ttacttgatg
    17821 aaccactgaa caatttggat atgaaccatt ccgtacagat gatgaaggta cttagaggtc
    17881 tttgtgatga attagataag actatagtat tagtaatgca tgatataaat tttgcatcat
    17941 gttattcaga taatatagtt gcattaaaaa atggaaaagt tgaaaaagta ggtcgtacag
    18001 atgagatagt aaatgaaaaa gttttagaag atatttatga gatgaatttt aacattaaga
    18061 atataaatgg aaatcgaatc tgtatttatt tttaaaatat tgtatatgaa aataaacttg
    18121 ctatatgatt ttttataaag tttttaaatt aaaataaaga ttaataggag agaggaaaat
    18181 attgatatga ataaaaaagc agcaatagta gcagcagtag ctattatagg attagtgaca
    18241 gtgtttgcat taggtggaag taaaaagaat gaatcaaaaa catcagaaaa ttcaaataat
    18301 actatgaaga taactcataa cttgggagaa acagatgtaa aattaaatcc taaaaaagta
    18361 gtggtatttg attattcggc tttagataca atggatgcat taggtgtagc tgagacttta
    18421 gtaggacttc caaaagcgag tttaccagca tccttagaaa aatataagga tgacaagtat
    18481 gcagatttag gaggattaaa agaaccagat ttagaaggta taaagtcggc aaatccagat
    18541 ttaattataa taaatggaag acaagaagat ttttatgagc aattgtcaaa aatagcacca
    18601 acaataagta caagtaaaga tgataagaag tacttagaat ctgttaaaaa taatatagac
    18661 aaaatagcaa aaatatttgg agtagaagaa aaagcaaatc aagaatttag taaaattgaa
    18721 aagaaaatag aaactttgaa taaaaaagta acagataaaa atttaaatgc tttaactata
    18781 atggtaaatg aaggtaattt aagtgtattt ggtgaagaat caagatttag tatattatac
    18841 aatagttttg gatttgaaaa taaagataaa aatataaaag aatcaagtca tggacaaaat
    18901 attacttttg aatatatagc taaacaaaat ccagaagtta tgttcgttat agatagagga
    18961 atagctactg gcagtgatgt aaaagaaagc tcaactgcaa aatctgtttt gaacaatgat
    19021 ataataaaat ctatggatgc atataagaat gataatataa tatatttaga ttcaccaaca
    19081 tggtatgtaa atgatggtgg tctaacatct ttaaataaaa tgatagatga tgcttcaaaa
    19141 gcggtaaatt aataatccga tataaccaaa atgtattttt tataaaatga ttatcaaata
    19201 aactaggagt ttttaagaac tcctagttta ttttttgcat aaaaaagata tagtttaaac
    19261 aataatatta tctataataa gataaatcta ttcaaaatct aataaaatat gaagttatac
    19321 tagacaaaaa gaataactc
//
LOCUS       DAMJVK010000075        18975 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.076, whole genome shotgun sequence.
ACCESSION   DAMJVK010000075 DAMJVK010000000
VERSION     DAMJVK010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18975
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.076"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(71..607)
                     /locus_tag="PC414_002700"
     CDS             complement(71..607)
                     /locus_tag="PC414_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HDF2465088.1"
                     /translation="MEYKSPVYGIIAVPIEKIESNDYNPNAVAPPEMQLLYKSIKEDG
                     YTMPIVCYYNEEKDKYIIVDGFHRYRVMLENEDIYDRENGLLPVSVIDKSLDERMAST
                     IRHNRARGSHNVDLMSKIITELCEIGKSDEWISKHLGMEVDEILRLKQITGLAYLFKD
                     KEFGNAWIPFGSDIEEEN"
     gene            complement(625..1938)
                     /locus_tag="PC414_002701"
     CDS             complement(625..1938)
                     /locus_tag="PC414_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HDF2465089.1"
                     /translation="MDKRYLESDVFEAFQERLSYLFNEFDYIYVSFSGGKDSGLLLNL
                     VLDYKRKNNISSKIGVFHQDFEAQYSLTTEYVEKVFEDNIEDIEPYWVCLPMATRTAV
                     GNYEMYWYPWDDEKEELWVREMPVKEYIINQKNNPFTYYKYKMRQESLAKQFGRWIRD
                     IHEGAKVVCLLGTRAAESLQRYCSIVNKQHGYKGKCWITKEFKNVWTASPIYDWEIED
                     IWLAHYKFDYDYNKIYDLFYKAGVAPRKMRVASPFNDSAKESLNLYRILDPDVWVKLV
                     ARVQGVNFTSIYGKTKAMGYRSVKLPYGHTWKSFTYFLLDTLPENIRNSYIKKFSTSI
                     EFWQNTGGGLSEIVIRELLRKGYKIELNGVSNHTKDKKSRVIFLDDIPDDTDDIKFTK
                     DIPSWKRMCYCILKNDHMCRFMGFGPTKEQRIRTEYIKKKYEKIH"
     gene            2293..2733
                     /locus_tag="PC414_002702"
     CDS             2293..2733
                     /locus_tag="PC414_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HDF2465090.1"
                     /translation="MYERMLDKSNKPCLSQIIEYIGKDGYARLCKLETYLKTQYNLSR
                     ELRFPFGNNYGWGYKYSHKSFHLFYAFFEKGAFTVLIQIGDKQVPAVEKTLVALTPKA
                     KELWVTRYPCGNNGGWIKYRILEDADLLDIIQYIRAKKSPPKQN"
     gene            complement(2917..3105)
                     /locus_tag="PC414_002703"
     CDS             complement(2917..3105)
                     /locus_tag="PC414_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDF2465091.1"
                     /translation="MNKNNNLVIICMFIGMILGMAIGCAIGISKGNVGITMCYGLIFG
                     MIIGICIGTIIKNSNKKE"
     gene            4127..4522
                     /locus_tag="PC414_002704"
     CDS             4127..4522
                     /locus_tag="PC414_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465092.1"
                     /translation="MNILKMKKTGIFLGGVLFGTVGVKILSSDCVKKTCTRATAGALK
                     VKDSVLETTTKIQENVEDILAEAKEINEQEDLSTSKVNVVQDTLKCEQEVLKTETIKT
                     VEDTNSNSIKCEEIKDNVIEEKNSTCTTE"
     gene            4565..6664
                     /locus_tag="PC414_002705"
     CDS             4565..6664
                     /locus_tag="PC414_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HDF2465093.1"
                     /translation="MNFYIKHEIRGRIRFDLQLGKLNNKQSDTLLYYLTSINGITKAK
                     VYDRTGDAVIYYTGEREYIIREITKFSFNNSKIENLVPEHTSRELNNYYRDKLANKLL
                     VKSISKIFFPKSLRFFLIGLHSIKYFKKGLSSLLNKKIEVSVLDATAIGISIFRKDIN
                     TAGSVMFLLGIGELLEEWTRKKSIDDLAQSMSLNIEKVWLRKGDTEILIPISEIKEGD
                     LVSVTMGNMIPLDGVIVSGETMVNQASLTGESLAVNKKEGSYIYAGTVIEQGNIVMCV
                     KEKAGTTRFQKIVTMIEESEKLKSSVESKFEHLADTLVPYSFLGSILTYAITRNPIKS
                     LSILMVDFSCALKLSIPISVLSAMRECNNNNITVKGGKFLEGVACADTIVFDKTGTLT
                     KAQPTVSDIITFQNYNKEDMLRLAACLEEHFPHSIANAVVYEAEKQGLSHKEMHTEVE
                     YIIAHGISTKVDDKKVVIGSKHFVFEDEKCTIPDGEEDKYNNLSDEYSHLFMAISGKL
                     SAVICINDPLRKEAKYVISNLRECGIKKIVMMTGDSEKTAKSIASKVGVDEYYSEVLP
                     EDKANFVKSEKLKGRKVIMIGDGINDSPAISESDVGIAMSEGAEIAREISDITISADN
                     LNNLIVLKQISNKLMKRVDLSSKFIIGFNLGLILLGVGGFVRPSTSAFLHNTSTVGIS
                     LNSMTNLLENTNTYNYH"
     gene            complement(6889..12099)
                     /locus_tag="PC414_002706"
     CDS             complement(6889..12099)
                     /locus_tag="PC414_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HDF2465094.1"
                     /translation="MANKLYSEIVNLLEEGRDELRKYDLKEKSILLFLGASGVGKSTC
                     INYLKGCVMEEKTDEETGQIYITAKDSAVEIGNGVYSKTLCPEVVDIANRDFSLCDCP
                     GFFDNRGAEYMIAGAMLVRETISTSSKVKGMVVILDYQSLLDSRHTLLIETGKNLKDM
                     LGDYAKYKENFFFLINKIPKTLIGNVTEEKIRNILEKAKEDLDSQEKDYDGCKELVYL
                     LNVALSDGVSIKLCNPLDKQPEKLLDEMKNLPETFEKTDLGLMLTASCKIEIQKAVDV
                     TYKDLEKLLVPYIESVKKGMEDSISILSSINSLKDKKIQLDKMYTDLCSRTNNMKELM
                     EFVQELSYGDGVQKVNILEETRLLLELNSYCSETHIVGLSELLKSLLKNTLEDTLKDI
                     KYWIAEEEYREEEAKKLEDAEYRIEKIQSCTYIKSYEKENVLSIYIPIPKVNLSDLTK
                     IISGYQLNILSSIYMYGDCELKIDCNMEKEVFSGKNIVLLTEKLHIPEGTYSINVSGK
                     DAEKLVGESSIYDGMNGRNGNNAGTSGDIILSFKEIEKRGELKLYVDGGRGGNGQDGK
                     KGDTGITGEAGANAKIGSKKESKEFANVKGTKYYVVKAEKGKQGGTGGTGGNGGNAGL
                     GSKSGQVYLLGKAPLLKSAISKKDGNHGLAGKGGSGGAGGAGGLGGIRGIYEETIRYI
                     GTSPPMDNSDRNYYYFDRYVFNHALNQKQAADDGEKLNTLYEEHKDWTISKREDSGSA
                     GAEGKAGHSGDALKEKELLKQEYSLSIDTFFYEFFERFGFSGIKGYRNLRLLCAVGIL
                     EADILEFSHIYEIFYAVEKIEMQLNREILQKYYSFEKKNKAVLSMDDMNIKKEFYKEA
                     KEIYEYLINKLMQSDRIESFVGKNQNFSEWSMILFALEQKTAFYVQMIEESEIGGNRV
                     TDIRSLTDYLDKTLRELEQGKKDELKEGYFNTYKESILTQIESGNQQIEVLKNELENK
                     MLSVNAKMNLLIQEIIKMKEDEQEDIEELKKKQAKLEEQMKTQLILSGIQSALSLLTA
                     FSGVGQACLGAFDSAKSLKENLSAKEEEYQWTNRMVDFSDFKLDSDGMRFVLGEQLSE
                     DRKNALDKMTYNLAGVDEERRTKFDQIKEIIVKSIDDDNYADKADDIQRAKEGLSESE
                     YKEIYTKIQKKEIGMLEANYNNKQQKDKLAALEKSKQAIERQQKTSKIKNALSIGSSA
                     CSLINTAISGVNNCLNVKSSFESKIEVVKEAIKDKENNIKKLDELNTQMVEFKDKTLK
                     ESVIPFVEKFDSTVKNQDIFENEISKLKMKEVLTELSTKFGSLKMENSEEISIIFKKV
                     QDMMETQIIITDKIDKKKDNLAMGELIYQMSDSGNQMNEYQKKLIHQIELNQVRYLCQ
                     VEAAAFQLWTFPFGSNIVNILNRCHVGITEDENEVINQAKEQNRQIREFLDQDAYQWQ
                     KTDNHILKVALTGSLDGKSRVGFENTYSLYDCNEEEVKKLLTGDEISFEVPLNKNFDA
                     IKYVTMYANIPNLRRNILEDSLKVKVHIHLYDSGYFSLYDDKKDVYYQYVFPQNYINI
                     THGLDIKYNDSDSINSIWSSVEDKGIEKFKNAGLNSGRSPYSMAKISLKAHFNKKLSY
                     DSLTNEQKKKLESYFDKPSKYAWSDSIDILRSLGYIDLKGILSKSWYEIKKDIDSDGE
                     IMKHFSILQVDLLERIIYEQTSILKENIPNLTIELVGCGIYVESEFNYLKQNDLKCYE
                     ERLVENTIIG"
     gene            complement(12114..14609)
                     /locus_tag="PC414_002707"
     CDS             complement(12114..14609)
                     /locus_tag="PC414_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2465095.1"
                     /translation="MPENNNQLLEEITLLLNEGKVAINKAENQNLILILGGTGCGKST
                     AINYLAGCVMMEVEDEDTGISYIDCLDPVAEIGSGAVSQTLYPNIVNMRKPIEGMDAK
                     LCDTAGFSDNRGVAHDISAAVSLGEVFKNSASICAVIVLIPEADILDSRLTKVLDLFQ
                     QLNMCLHKENFKGSITFFISKSFLGRTEKQIYNVIKRKYEQLCENLDSNIEEKAWLFE
                     ELLKDNGKNIHLCKPLASDEREGLLKQVLELDEVKDKTSAFQYPISADARLVLDTLIK
                     EIQNNIVSSLEDYVKAYTEDFINIIGSITMIQEIAKYDEVVKVFKNWLESTTSLTIES
                     FINVLAKQSGLFLQDSCMKLQNKIKGNLDIIDALSKYTEHGTGEILLPSVHMEFIIEK
                     ASKKIMEQNFIIIRKNLEKVLVSYEVQKQVFTLPNREKLMEVANKKDYVYSVSDFNSI
                     LTNVTVTQNEELEDSITRLSAYGEEGNEIIREFIGKYILNPFIVKTININDKKGIEIR
                     ALIPNLVISSVLEYFKNRKVDDNVSTFMFFAKDTIYMDDNLGMNFASEKNIILASNNL
                     DVGSNVKINVSGKSGELVDNSMYGKNGEDGRDAGNIYLYIKEEIRNYRLTLIANGGNG
                     SKGIDGENGETGASGSNGADAGYTSYFHGQMFKGYFEKGTSGKKGEKGGAGGNAGRGG
                     NAGKKGNIYFISDSEEQKNRIINHCDITVKDGEAGPNGRPGLGGYGGSGGYHGYNTLS
                     FSPSGIDKTVYYTGYFTSWDYKLDSIIKYHIEFSGGSWNEGKTGPAREYAEYGDRGDN
                     GGFNAPNTKLSTAVSKVEENLIKEKYQGYCSEI"
     gene            complement(15270..15557)
                     /locus_tag="PC414_002708"
     CDS             complement(15270..15557)
                     /locus_tag="PC414_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465096.1"
                     /translation="MRLNITKQNTIEVENSRKEDVYFVIVDNVFFERQGDVFNSITNN
                     NLNNYDVKILPTDNIFLELKITSTKDLEPVFIVVEDRQYNHGSIMFKPVLV"
     gene            complement(15577..15852)
                     /locus_tag="PC414_002709"
     CDS             complement(15577..15852)
                     /locus_tag="PC414_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465097.1"
                     /translation="MSLFVGGWSKFRAVEEEDLKVFNEAVGMLEGVGYEPLIVSTQVV
                     AGTNFKFICNATSVTNPPQNYLAEIVVFKPLPCDEHNATITSINIIK"
     gene            complement(16487..16666)
                     /locus_tag="PC414_002710"
     CDS             complement(16487..16666)
                     /locus_tag="PC414_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465098.1"
                     /translation="MGKDRFEKVYSQGKINATEIWVDKETGVNYVFHYYGNAAGFTPL
                     LDREGKPVISPIVSK"
     gene            complement(16951..18426)
                     /locus_tag="PC414_002711"
     CDS             complement(16951..18426)
                     /locus_tag="PC414_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2465099.1"
                     /translation="MKRKPQYLIIFEHIIQQIYTGKITIGDRLDSIKSMSEEYMVSKN
                     TIKTVIKMLSEKGFIETKAGSRPVLIQSTSKQSIESDLLYEKILQISEIYKIFSLIFP
                     SIAMYNIKRFTSKDFEELNEIIDCMEKNSSNYLVFKEQRLKYINKLISKTNNFLIHHF
                     CEIIQHDVIISQLAYNFDNLDNLDNCCIDFFRNKYIEQVRKTYLYGLDGNLIEFKKLL
                     TYMYKACRESILKMVENRAPQSIDTSSFNSVTLEKCYLYDLIVSDVICQIFEGNIKIG
                     DCLPSITSIINRYNVSLPTVRSAYNELNELGIAKTINGKGTYVTLFSEYDDDYINTEE
                     GAKRLSLLLDAMEFVTITLEDVVLLLGNKMDLIAVDKIETHLRYLQNNCEECKVYPDF
                     VLLCKIVDYTQIYVLQETFLHLKQYMIFGIYLERFFHEEYNEILKTRFDVCFEILRYL
                     KKGDVERFAESFSNIFKESLQYLKKVISSLNVDMRLKLGIL"
BASE COUNT         6303 a         3320 c         2074 g         7278 t
ORIGIN      
        1 aactctttat tttttaatat atattttaaa ttttcttttt taataaatct ttttctttta
       61 ataaatctat ttaattttct tcttctatat cagagccaaa gggaatccat gcattaccaa
      121 actctttatc tttaaataaa taagctaatc cagttatttg cttcaatcta agaatttcat
      181 caacttccat acccaggtgc tttgatatcc attcatcaga ttttccaatt tcacaaagtt
      241 cagttataat tttactcatt aaatccacat tatgagaacc tcttgcacga ttgtgtcgaa
      301 tagtacttgc cattctttca tcaagagatt tatcaattac tgaaacagga agaagtccat
      361 tttctctatc atatatatcc tcattttcaa gcattactct atatctatga aaaccatcta
      421 caataatata cttatccttt tcctcattat aataacagac aattggcatt gtatacccat
      481 cttcttttat acttttatac aacaactgca tttctggtgg ggctactgca tttggattat
      541 agtcgttaga ttctatcttt tcgattggca ctgcgatgat tccatataca ggacttttat
      601 attccatagc atcttaactc ctatctaatg aattttttca tatttcttct taatatattc
      661 cgttctaatt ctctgttctt ttgtaggacc aaatcccata aatctacaca tatggtcatt
      721 ttttaaaata caataacaca ttcttttcca acttggtata tctttggtaa atttaatatc
      781 atccgtatca tcaggtatat catccaaaaa tatgactcta ctctttttat cttttgtatg
      841 atttgaaact ccatttaatt ctattttata accttttctc aacaattcac gtataactat
      901 ttctgacaaa ccaccacctg tattctgcca aaactcaata gatgttgaaa attttttaat
      961 atagctattc cttatatttt caggtaaggt atcaagcaaa aaatatgtga aacttttcca
     1021 tgtgtgtcca tatggcaatt taacactacg atatcccata gcttttgttt tgccataaat
     1081 agaagtaaaa ttaactcctt gtactctagc aaccaatttc acccatacat ctgggtccaa
     1141 tattctgtat aaatttaaac tctcttttgc tgaatcatta aaaggtgatg ccactctcat
     1201 ttttcttgga gctactccag ctttatagaa taagtcataa attttattat agtcatagtc
     1261 aaatttatag tgtgctaacc atatatcttc tatttcccaa tcatatatag gagatgccgt
     1321 ccagacattt ttaaattcct tagttatcca gcactttcct ttatatccat gctgtttatt
     1381 cacaatagaa caataacgtt gtagagattc tgctgctcgt gttcctaata agcaaacaac
     1441 ttttgcacct tcatgtatat ctctaatcca tctaccaaat tgttttgcca aactttcttg
     1501 tctcatttta tatttataat aagtaaaagg attatttttt tgatttatta tgtattcttt
     1561 gactggcatt tcccttaccc ataactcttc tttctcatca tcccatggat accaatacat
     1621 ttcataattt cctacagcag ttcttgtagc cattggtaaa cacacccaat atggttcaat
     1681 atcttcaata ttatcttcaa atactttttc aacatattca gtagtcaaac tatattgagc
     1741 ctcaaaatct tgatgaaata ctcctatttt ggaacttata ttattctttc tcttataatc
     1801 caaaactaaa tttagcagta aaccactatc ttttcctccg ctaaaagata catagatata
     1861 atcaaattca ttaaaaagat aagaaagtct ttcttgaaaa gcttcaaata catcggattc
     1921 taaatatctc ttatccattt gaattatccc ctttcaatta gaagtttata aaaccttaca
     1981 atgcaatatt attatgcttt tttagcagta atcagcataa attatacatt atttaaatac
     2041 acaaatccaa ctaactacat ataattataa tgtaattata cttttgtatt ataaactata
     2101 ttttatcatt attttacatt tttttccaat atatataaat atcatatttt tctattagct
     2161 aaaactaatt actttatatt tctacgtatt agtataataa tacataaaaa cttgaataat
     2221 gcataaaaac ttgacatact gttgtcataa ttactttgct ataattaaaa gtaaaaatgg
     2281 aggtaaattc taatgtatga acgtatgctt gataaaagca ataaaccctg tttgtcccaa
     2341 ataatagaat atataggtaa agatggatat gcacgattgt gtaagttgga aacttacttg
     2401 aaaactcaat ataatttatc aagagaactt cgatttcctt ttggaaacaa ttatggttgg
     2461 ggttacaaat atagccataa atcttttcat ttattttatg ctttttttga aaaaggtgca
     2521 tttactgttc tgattcagat aggtgacaag caagttccag cagtagaaaa aacgttggta
     2581 gctttgacac caaaagctaa agaactctgg gtcactcgtt atccttgtgg caataatgga
     2641 ggctggatta agtatcgtat tttagaagat gcagacttac ttgatattat tcaatatata
     2701 agagcaaaaa aatctccacc taaacaaaat taacttttat tttttgtgat ttaaatatag
     2761 tgtatataaa ttccattctt tccatattaa aaaataatat aaaaaatatc tattagtata
     2821 acttatttag tatgtcttat tctacttttt acagataatt aaaaagtttt atcataaata
     2881 aatcataact agacatccta gtaaatcaaa attaatttat tcttttttat tcgaattttt
     2941 gataatagtc cctatacata tgccaattat cattccaaaa attagaccat aacacattgt
     3001 aatacctaca tttcctttag aaatacctat agcacaacct atagccattc caagtatcat
     3061 gcctataaac atacatataa taactaaatt gttgttttta ttcatagttt ttctccattc
     3121 aatatatttt aaattgccta gctcgccttc ttagcttaaa acaaagaaat ataaattaac
     3181 tttctttcat tctaatatct gcctgtataa atgtcaaaca acacatttct tgtcataacc
     3241 tcctcttttt gaagtaagta caatctccca gacgcaataa tatcaatttc ctaaaaccat
     3301 ttaagtaact tttatattta cataatattt acaatcataa actatattgt tattcattta
     3361 tacatcataa acactccctc ctaatcatta ttatacttaa aataatgttc atttataata
     3421 atatatctaa cgcactttaa ttgaaaaaac taaaactttg cctttcattc ccatatataa
     3481 ataagtatac tttttgatgc tctattacaa atcaactaaa ttaattgata taaattatca
     3541 ttttctgttg aaaataccat tcgcacatgc tatacttaat tgcataaatg gtaataaatg
     3601 tcatatgatt ttacaaatct attaataaca attattactt ttactgtatt ttaaagtttt
     3661 tggactaaat atcaagaact ttctaaaata caaagtacaa aaaatttata taagttataa
     3721 atttatatga actagataag ctcaaacaat atgttatgta taaactgtgt agtattcggt
     3781 tataaattta tataaattat ataagctaga taaagtatta cctcaactat ataagctaga
     3841 tatataaatt gcttatataa gctagataag aattatttca actatataaa ctacatatat
     3901 aaattacata cataagctag atatataaac tgcatatata agctatataa gaattatttc
     3961 aaccatataa actacacatg taaaattcat ataaatttac taatataaat caaataaaat
     4021 tactaaactc tctaaactaa ttttgtaaaa catactctat ttttaacatt tgttacctta
     4081 tacataagta caaacaaaat ctaactaaat gaaaggataa tgtaaaatga atattttaaa
     4141 aatgaaaaaa acagggatat ttttaggagg agtacttttt ggtactgtag gtgttaagat
     4201 actttcaagt gattgtgtta aaaaaacttg cactcgtgca acagcaggag cattgaaagt
     4261 taaagattct gttcttgaaa caactacgaa aattcaagaa aatgtagaag atattttagc
     4321 tgaagcaaaa gaaatcaacg agcaagaaga cttatctaca tctaaggtaa acgttgtaca
     4381 agatactctt aaatgcgaac aagaagttct taaaactgaa actataaaaa ctgtagaaga
     4441 tactaattct aatagcatta aatgtgaaga aatcaaagat aacgtaattg aagaaaaaaa
     4501 tagtacttgt acaacagaat aaatttaatg attattatga ttaataatat caggaggaag
     4561 taaattgaat ttttatataa aacacgaaat aagaggtcgt atacgctttg acttacaact
     4621 tggcaaatta aacaacaagc agtccgatac tctcttatat tatttaactt ctataaatgg
     4681 tattaccaaa gcaaaagttt acgatagaac tggtgatgca gtcatttatt acactggtga
     4741 aagagaatac ataataagag aaatcactaa attcagtttt aacaatagca aaatagaaaa
     4801 cctagttcct gaacatacca gcagggaatt aaacaattat tatagagaca aattagcaaa
     4861 taaactgcta gtcaaaagta tctccaaaat attttttcca aagtccttaa gatttttttt
     4921 aattggactt cattctataa aatattttaa aaaaggtctc tcttccctgc taaataaaaa
     4981 aatagaagtt tctgttttag atgccactgc tataggtatt tctattttta gaaaagatat
     5041 caatacggct ggttcagtta tgtttcttct tggtatcggt gaactattag aagaatggac
     5101 tagaaaaaaa tctatagacg acttggctca aagtatgtca ctaaacattg aaaaagtatg
     5161 gttaagaaaa ggtgacactg aaatcttaat tccaatttca gagataaagg aaggagattt
     5221 agtaagtgta actatgggaa atatgattcc attggatgga gttatagtat caggagaaac
     5281 aatggtcaat caggcatcac ttacaggtga atcccttgcc gttaacaaaa aagaaggctc
     5341 ttacatatat gctggaactg taatagagca aggcaatatt gttatgtgcg tcaaggaaaa
     5401 agctggtact actagattcc agaaaatagt aactatgatt gaggaatctg aaaaactaaa
     5461 atcttctgta gagagtaaat ttgaacatct agcagataca ttagttccat acagcttttt
     5521 aggaagtata ttaacctatg caattactag aaaccctata aaatcactct ctatattaat
     5581 ggtagatttc tcttgtgcat taaaactctc tatccctatt tcagtactat cagccatgag
     5641 agaatgtaac aataacaaca ttactgtaaa aggtggaaaa ttccttgaag gtgttgcatg
     5701 tgcagatact attgtatttg ataagacagg cacattaaca aaagctcaac caactgtttc
     5761 agatataatt acattccaaa attataataa ggaggatatg ttaagacttg cagcttgctt
     5821 agaagaacac tttccacact ccatagcaaa tgcagtagtt tacgaagctg aaaaacaagg
     5881 gcttagtcac aaagaaatgc atacagaggt agaatatatt attgctcatg gcatttcaac
     5941 aaaagttgat gataaaaagg ttgtaatagg tagcaaacat tttgtatttg aagatgagaa
     6001 gtgtactatc ccagacggtg aggaggataa gtacaacaat ctatcagatg agtattcaca
     6061 cctatttatg gcaatatcag gtaaattatc agcagttatt tgcataaatg accctttacg
     6121 aaaagaagca aaatatgtaa tttcaaattt aagagaatgc ggaataaaaa agattgtgat
     6181 gatgactgga gatagcgaaa aaacagctaa atctatagca agtaaagttg gtgttgatga
     6241 atattattca gaagtcttac cagaagacaa agccaatttt gttaaaagtg aaaaactcaa
     6301 aggtcgcaaa gtaattatga ttggtgatgg aatcaatgat tctcctgcta tttcagaatc
     6361 agatgtcgga attgcaatga gtgaaggtgc tgaaatagca agagagattt cagatattac
     6421 catatcagca gataatttga ataatttaat cgttttaaag cagattagta ataagcttat
     6481 gaaaagagtt gatttaagtt ctaagtttat tattggcttt aatttaggtc tgattttact
     6541 tggtgtaggt ggatttgtta gaccaagtac atctgccttt ttacacaaca catctactgt
     6601 cggaataagt ttaaacagta tgacaaattt attggaaaac accaatactt ataactacca
     6661 ttaatctcca ttacataaaa catatgatat aaaaaattta taattcatct ttttaaaaga
     6721 atatgtctta aaattagctt ttttctaatc aaaattatat aaagcatact ttacaaattt
     6781 taaactcaac ttatttctat tttttacaaa aaaggagatg attcaaattt tcattttgaa
     6841 acatcccctt ttgtaaattt ctttaaaatt tatatattaa ttatattttc aaccaataat
     6901 tgtattttca accaatcttt cttcatagca ttttaaatca ttttgcttta gataattaaa
     6961 ttcagactct acataaatac cacagcctac caattcaatt gtcaaattag gtatattttc
     7021 ctttagtata ctagtctgct cataaataat tctttccaat aaatcaactt gtaatataga
     7081 aaaatgcttc ataatctccc catcactatc tatatctttc ttgatttcat accaactctt
     7141 tgacaaaatt cccttcaaat caatataccc tagactcctc aatatatcta tactatcgct
     7201 ccatgcatac ttactaggtt tatcaaagta tgattctagt ttcttcttct gctcatttgt
     7261 aagtgaatca taagaaagct tcttattaaa atgtgccttc aaagaaatct tagccataga
     7321 atatggactt ctaccagagt ttaatcctgc atttttgaac ttttcaattc ctttatcttc
     7381 tactgaagac catattgagt ttattgaatc tgaatcattg tatttaatat caagaccatg
     7441 tgtaatattt atatagtttt gtggaaatac atattgataa tagacatctt ttttatcatc
     7501 atatagagag aagtatccac tatcatataa atgaatatgc acctttactt ttaaactatc
     7561 ctctaaaata tttcgtctaa ggttcgggat atttgcatac atggttacat attttatggc
     7621 atcaaagttt ttatttaatg gaacttcaaa actaatttca tctcctgtca aaagcttttt
     7681 cacttcttct tcattacaat catatagaga atatgtattt tcaaatccca cacgtgattt
     7741 cccatctaaa ctgcctgtta atgctacctt caaaatatgg ttatctgttt tttgccattg
     7801 gtaagcatct tggtcaagaa actctctaat ctgtctattt tgctctttcg cctgatttat
     7861 aacctcgttt tcatcttctg taatccctac atggcatcta tttaagatat ttactatatt
     7921 gcttccaaat gggaatgtcc atagttgaaa agctgctgcc tcaacctgac acaaatatct
     7981 aacctgattt aattcaattt gatggataag ttttttctga tattcattca tctgattacc
     8041 actatctgac atttgataaa tcaattctcc cattgcaaga ttatcttttt tcttatcaat
     8101 cttatcagta atgataattt gagtttccat catatcctgt acttttttaa atattataga
     8161 aatctcctct gagttttcca tttttaagct tccaaacttc gtgcttaatt cagttaaaac
     8221 ttccttcatc tttaatttac ttatctcatt ttcaaaaata tcttgatttt taactgtact
     8281 atcgaatttt tctacaaatg gtataacaga ttctttcaaa gttttatctt taaattccac
     8341 catttgagta ttcaattcat ctaatttctt aatattattt tctttatctt taattgcctc
     8401 tttaactacc tctattttac tctcaaaact agattttaca ttaagacaat tattaacacc
     8461 actaatcgca gtatttataa gagaacaagc agaacttcca atactcaatg catttttaat
     8521 tttagatgtc ttttgctgtc tttcaatagc ttgtttactt ttctctagtg cagctaactt
     8581 atctttttgc tgtttattat tgtaatttgc ctccaacata cctatttctt ttttctggat
     8641 ttttgtgtaa atttccttat actcgctttc acttaacccc tcttttgctc tttgaatatc
     8701 gtcagctttg tctgcatagt tatcatcatc tatagactta actataatct ccttaatttg
     8761 gtcaaattta gttcttcttt cttcatcaac acctgccaaa ttataagtca ttttatctaa
     8821 agcatttttt ctatcttcac ttagctgttc tccaagtaca aatctcatac catcagaatc
     8881 caatttaaaa tctgaaaaat caaccatcct gttagtccat tggtattcct cttctttagc
     8941 tgaaagattt tccttcaaag attttgcact gtcaaatgcc cctagacaag cttgaccaac
     9001 accactaaat gcagtcagta aactcaatgc gctttgtatt ccagaaagaa ttaactgtgt
     9061 tttcatttgt tcctcaagtt ttgcctgttt cttttttagt tcctctatgt cttcttgttc
     9121 atcttctttc atctttataa tttcttgaat caacaggttc atttttgcat taacagaaag
     9181 cattttattc tcaagttcat tttttaaaac ctctatttgt tgatttccag attcaatttg
     9241 agttaggata ctttccttat atgtattgaa atatccttct tttaactcat ctttctttcc
     9301 ttgttccaat tctcttaagg tcttatccag atagtctgtc aaagaacgaa tatctgtaac
     9361 acgatttcct ccaatttcac tttcttcaat catctgcaca taaaatgctg tcttctgctc
     9421 aagtgcaaat aaaatcatac tccattcgct aaaattctga ttttttccta caaaagattc
     9481 tattctatca gactgcatca atttatttat aaggtattca tagatttcct ttgcctcttt
     9541 ataaaattct ttctttatat tcatgtcatc catagaaagt actgctttat ttttcttctc
     9601 aaaactatag tacttctgaa gtatttctct atttaattgc atctctattt tctcaactgc
     9661 ataaaaaatt tcataaatat gtgaaaattc taaaatatca gcttctaaaa ttcctactgc
     9721 acacaacagt ctaagatttc tataaccttt aattccagaa aaaccaaatc tctcgaaaaa
     9781 ttcatagaag aatgtatcta ttgacaaaga atactcctgc tttaacaatt ccttttcttt
     9841 tagtgcatct ccagagtgac cggcttttcc ctctgctccg gctgaacctg aatcttcacg
     9901 tttgctaatt gtccagtcct tatgttcttc ataaagagta tttaatttct ctccatcatc
     9961 tgctgcttgc ttctgattta aagcatgatt aaacacatat ctatcaaaat aatagtagtt
    10021 tctatcacta ttatccattg gaggagaggt acctatatat cttattgttt cttcatatat
    10081 acctctaatt ccaccaagac ctcctgcacc accagctcct ccagagcctc cttttcctgc
    10141 taaaccatga tttccatctt ttttacttat agcacttttt aataaaggag cttttccaag
    10201 gagataaacc tgtccagact tgcttccaag acctgcattt ccaccattac ctcctgtgcc
    10261 tccagttcca ccttgttttc ctttttctgc ttttacaaca tagtattttg tacctttaac
    10321 attagcaaat tcttttgact cttttttaga acctatttta gcatttgccc cagcttctcc
    10381 agttatccct gtatctcctt tttttccatc ttgtccattt ccaccacgtc caccatctac
    10441 gtaaagttta agttctcctc ttttttctat ttctttaaaa gataatatga tatcacctga
    10501 agtacctgca ttattgccat ttcttccatt cattccgtcg taaatagaac tttctccaac
    10561 caatttttct gcatccttac cagatacgtt gattgaataa gtcccttctg gtatatgtaa
    10621 tttttctgta agtagtacaa tattcttacc tgagaagact tctttctcca tattacagtc
    10681 aattttcaac tcacaatctc catacatata tatagatgac aaaatattaa gttgataacc
    10741 agatattatt ttagtcaaat ccgataaatt cactttaggt ataggaatat atatagaaag
    10801 tacattttct ttctcatatg attttatata tgtacaactt tgaatcttct ctatcctata
    10861 ctctgcatct tcaagctttt tggcttcttc ttccctatat tcttcttctg ctatccaata
    10921 tttgatatct tttaatgtat cttctaaagt attttttagc aatgatttta acaactcact
    10981 tagaccaaca atatgagtct cactacagta actatttaac tctaaaagaa gacgtgtttc
    11041 ttctaatata ttcactttct gtacgccatc accatatgaa agttcttgta caaactccat
    11101 aagctctttc atattattag tcctactaca taaatctgta tacatcttat ctaattgaat
    11161 ctttttatct tttagactat ttatagaaga tagaatggaa atagaatctt ccataccttt
    11221 tttaacactt tctatataag gaactaaaag tttttctagg tctttatagg ttacatcaac
    11281 agctttttgt atttctattt tacaagaagc tgtaagcatc aatcccaaat ctgttttttc
    11341 aaatgtttca ggtagatttt tcatttcatc caacaacttt tctggctgtt tatctagtgg
    11401 attacataat ttaatagata caccatctga taatgctaca tttaataagt acactaattc
    11461 cttacaaccg tcgtaatctt tctcttgact atctaagtct tcttttgctt tttccaatat
    11521 atttcttatt ttttcttctg ttacattacc gattagtgtt ttaggtattt tattaataag
    11581 aaagaagaaa ttttctttat actttgcata atcaccaagc atatctttca aatttttgcc
    11641 tgtttctatc aataatgtat gtctagagtc cagtagggat tgatagtcta agataactac
    11701 catccctttc acctttgaag atgtggaaat agtttccctt acaagcatag caccagcaat
    11761 catatactct gctccacggt tgtcaaaaaa acctgggcaa tcacataatg aaaaatctct
    11821 atttgcaata tctacaactt ctggacataa agtcttagaa tatacaccat ttccaatttc
    11881 gacagctgaa tcttttgcag ttatatatat ttgtccagtc tcttcatccg ttttttcttc
    11941 catcacacag ccttttaaat aatttataca agtactcttt ccaacgcctg aagcacctag
    12001 gaataacaaa attgactttt cttttaagtc atattttcta agctcgtctc ttccttcttc
    12061 caaaaggttt acgatttcac tataaagttt atttgccata catccatccc tttctatatt
    12121 tcggaacaat agccttgata tttttcttta ataagattct cttctacctt tgaaacagct
    12181 gttgataact tagtatttgg agcattaaat cctccattgt ctcctctatc tccatattca
    12241 gcgtattctc ttgctggtcc tgttttacct tcattccagc ttccaccact aaattctata
    12301 tgatatttaa ttatactatc taacttataa tcccaacttg taaaatatcc tgtatagtat
    12361 acagtcttat ctataccact tggagaaaat gaaagtgtat tatatccgtg atagccacca
    12421 cttcctccat atccacctaa acctggtcta ccatttggac ctgcttctcc atctttcact
    12481 gttatatcac aatgattaat tattctattt ttttgttcct ctgaatctga aatgaagtaa
    12541 atattacctt tcttaccagc atttccacct cttccagcat ttccaccagc tccacctttt
    12601 tcaccttttt tgccacttgt acctttttca aaataccctt taaacatctg tccgtggaaa
    12661 taacttgtat atcctgcatc agctccatta cttccagaag ctcctgtttc accattttct
    12721 ccatctattc ctttagaacc atttcctcca tttgcaatca gtgttagacg ataatttcta
    12781 atttcttctt taatatataa gtaaatatta cctgcatctc taccatcttc accatttttc
    12841 ccatacatag aattatctac aagttctccg ctctttccac ttacattaat ttttacatta
    12901 ctacctacat caagattgtt agaagcaaga ataatatttt tctctgatgc aaagttcatt
    12961 ccaaggttat catccatata aatagtatct ttagcaaaga acataaaagt agaaacattg
    13021 tcatctactt ttctattttt gaaatattcc aatacactgc taattactaa gtttggaatt
    13081 aatgctctta tttcaatacc tttcttatca ttaatattaa ttgtttttac tataaatgga
    13141 tttaaaatgt atttaccaat aaattctcta attatctcat taccttcttc tccatatgca
    13201 cttaatcttg ttatactgtc ttctaactct tcattttgtg taactgtaac atttgtcaaa
    13261 attgaattaa aatcacttac actatataca taatcctttt tatttgcaac ttccattaat
    13321 ttttctctat ttggtaatgt aaatacctgt ttttgcactt cataagaaac taaaactttt
    13381 tctaaattct ttcttattat aatgaagttt tgttccatta tcttcttact tgccttttct
    13441 ataataaatt ccatatgtac agaaggtaat aaaatttccc cagttccatg ttctgtatat
    13501 ttacttaaag catcaattat atctaagttt ccttttatct tattttgtaa tttcatgcaa
    13561 ctatcttgta agaataatcc actttgtttg gctaatacgt tgataaaact ctctatagta
    13621 agacttgttg tactttccaa ccagttttta aatactttta caacctcatc atattttgct
    13681 atttcttgaa tcattgttat acttccaata atattgataa aatcttctgt atacgcctta
    13741 acataatctt ctaggctaga tacgatattg ttttgaattt cttttatcaa cgtatctaaa
    13801 actaatcttg catctgcact aattggatat tggaatgctg atgtcttatc tttaacttca
    13861 tctaattcta gtacttgctt taataagcct tccctctcat ctgatgcaag tggcttacaa
    13921 agatgaatat tttttccatt atcttttagc aattcttcaa ataaccaagc tttttcctct
    13981 atattactgt ctaaattttc gcataattgt tcatattttc tttttataac attatatatt
    14041 tgtttttctg ttcttcctaa gaaacttttt gaaatgaaaa aagttatact acccttaaaa
    14101 ttttctttat gaagacacat atttaactgt tggaacaggt ctagtacttt tgtaagtcta
    14161 gaatctaata tatctgcttc tggtatcaat acaattactg cacatataga tgcagaattt
    14221 ttaaatactt cacctaatga aactgctgca ctaatatcat gggctactcc cctattgtcg
    14281 ctaaaacctg cagtatcaca taattttgca tccattcctt ctattggttt tctcatattt
    14341 acaatgttcg ggtataaagt ttgagaaaca gctcctgaac caatctctgc cacagggtct
    14401 aagcaatcaa tatatgatat accagtatct tcatcttcta cttccatcat tacacatcct
    14461 gcaagatagt taattgcagt acttttgcca catcctgtcc caccaagaat tagaattaaa
    14521 ttttgatttt cagccttatt tattgctact tttccttcat ttaataaaag tgttatttct
    14581 tctaaaagtt gattgttatt ttctggcatt tattttcccc cttggttata tattttattt
    14641 aaataaagtt taatccctta agtagattgc aaagttttta gactatattt aaatttgttt
    14701 tttatttttt atttttaata aatttgcatt ttcactaaat aattattctt ctttgtttat
    14761 taaaataact gtgttgtata atataatctt tatcaacaca gttattttat ttaatgcgct
    14821 tattttattg tattttatgt aatacagttg ttttttatat tatatgtcca ttatttttct
    14881 atggttatta catatagatt tttaattata tttgtaccat taatttaaac attcctatac
    14941 tactgcttaa gttttacacc tataccagct tgaatatttt atttttattt gcttaaatag
    15001 ttcaactata ctggtttaaa taggttatct ttatttgctt aaatagttca actatactgg
    15061 ttaaataggt tatctttatt tgcttaaata gttcaactat actgatttaa ataggttatc
    15121 tttatttgct taaataagtt caactatact gatttaaata ggtttccttt agtttaaata
    15181 attcacctat actggtttaa ataggtttcc tttagtttaa ataattcacc tatacctact
    15241 tgaatagttc acttttattt gcttaaattc tataccaata ctggcttaaa cattatacta
    15301 ccatgattgt attgtctatc ttcaactaca ataaaaacag gttctaaatc tttagtagat
    15361 gttattttta attctaaaaa aatattatca gttggtaata tttttacatc ataattattt
    15421 aaattattat tcgtgatact attaaataca tcaccttgac gttcaaaaaa aacattatca
    15481 actattacaa aataaacatc ttcttttcta ctattttcaa cctctatagt attttgttta
    15541 gttatattta gtctcatctt ccacctccta ttaatattac ttaattatat taatacttgt
    15601 aattgttgca ttatgttcat cacaaggaag aggtttaaaa acaactatct ctgctaaata
    15661 attctgtgga ggatttgtaa ctgaagtagc attgcaaata aatttaaaat ttgttcctgc
    15721 tacaacttga gtagaaacaa taagtggttc ataaccaacc ccttctaaca ttcctacagc
    15781 ttcattaaat acttttaaat cttcttcttc gacagctctg aattttgacc atccaccaac
    15841 aaaaagactc atataaaaac ctcctttata tatatattta tttatatata gtagtaccaa
    15901 ttttctcaat attcaatatt ttctgtgtaa ctggtcaatg aaaatgcata actggataaa
    15961 aatacttata aaataactta actttacata taaatagctt actttacatg taataagatt
    16021 attctacaat atgaaatgca tcttgttttg aatatattct aaatacaata atggatattt
    16081 tcaattatta taattagaaa atatccatta cttaacttgt tttatttaaa atcacataga
    16141 gactttatta ttttttttat tgcatgaata gctatttcag gtatcataag gtgatatacc
    16201 ccctccttga aaccacaaaa aacatgacaa actttacgaa tcttgtctta aaaaactaca
    16261 ggtacattcc ttatttgtgg attggagtag ccataacaag caaattttac aatttttatg
    16321 atgacaaaaa tcaataaaat cgcttagtat ataacacgaa atactacttg ttactattaa
    16381 gaatagttag ataggaagta tgcattatac aaataaagga atcaataaag aaagttaaaa
    16441 catccctaca aattaaaaat ctgttacatt tttctatatt taaacgttat ttacttacaa
    16501 ttggagaaat aacaggtttt ccttctcggt caagtaaagg cgtaaatccg gctgcattgc
    16561 cataatagtg gaatacatag ttcacacctg tttctttatc cacccaaatc tccgttgcat
    16621 ttattttccc ttgtgaatat actttttcaa atctatcttt tcccatttta taatcctcca
    16681 tatttttgat tggtctatgc cttcttcact taagtagaaa aaatacataa cttatttttt
    16741 taattctaat atgagcacgt aaaaatgtca aacagcttat ttaatttcat aacttcatgt
    16801 tactgttcgt aaccttgtct gtttacttac actgcattac agcaccattt tatcctttac
    16861 attattgtat tgcacacagt aaaaatgctt actttatggg atttttcaac taaaaaaagg
    16921 attttatctc cacaaaacat ttactatttg ttacaatata cctaatttta atctcatatc
    16981 aacatttaat gaagatataa cctttttcaa atattgtaaa gactccttaa aaatattaga
    17041 aaaagattct gcaaatctct caacatctcc tttttttaaa tacctaagta tctcaaaaca
    17101 tacatcaaaa cgtgttttca aaatttcatt atattcttca tgaaaaaatc tctctaaata
    17161 gattccaaat atcatatatt gttttaaatg caaaaatgtt tcctgtaata cataaatttg
    17221 tgtataatct actatcttac aaagtaaaac aaaatctgga taaaccttac actcttcaca
    17281 attattttgt aagtacctta aatgagtttc tattttatct acagctatca aatccatttt
    17341 atttcctagt aaaagtacta catcctcaag agtaatcgta acaaactcca tagcatccaa
    17401 aagcaaactc aaccttttag ctccttcttc tgtatttata taatcatcat catattcaga
    17461 aaaaagcgtt acatatgttc ccttgccatt aattgtttta gcaattccca attcatttag
    17521 ctcattatag gcacttctaa ctgttggaag tgatacatta tatctattaa ttatcgaagt
    17581 aattgatggt agacaatctc ctattttaat attgccttca aaaatttgac aaattacatc
    17641 tgaaacaatt aagtcatata aataacattt ttccaacgtt acagaattaa aactacttgt
    17701 atctatactt tgtggagcac gattttctac cattttaagt atggattctc tacaagcttt
    17761 atacatatat gttaatagtt ttttaaattc aattagattc ccatccaatc catataagta
    17821 agtttttctt acctgctcta tgtatttatt acgaaaaaag tctatacaac aattatccaa
    17881 gttatccaag ttatcgaaat tataggccaa ttgtgaaata atgacatcat gctgaataat
    17941 ctcacaaaaa tggtgaatca ggaaattatt tgttttactt attagtttat ttatatattt
    18001 tagcctttgt tctttaaaga ctaaataatt agaactattt ttttccatac aatcgataat
    18061 ctcgtttaat tcttcaaagt ctttagatgt aaatctttta atattgtaca tagcaataga
    18121 aggaaatatt aaactaaaaa tcttatatat ttctgatatt tgtaaaatct tctcatataa
    18181 taagtcagat tctattgatt gtttactcgt actctggatt aaaactggtc tagaaccagc
    18241 ttttgtctct ataaagccct tttctgagag catttttatt acagttttta tagtattttt
    18301 tgataccata tattcttctg acatgctttt tatagaatca agcctatctc caatagtaat
    18361 tttaccagta taaatttgtt gaattatatg ctcaaaaata attaaatatt gtggttttct
    18421 cttcatattt ttcctcttcg ataaattaga tttattgttt tttgtaaggt atatttactt
    18481 ttatttaata gggcaaaata gttgcttatt ttttcctcat aatctataat taatataaac
    18541 catttatata tatggtctaa gttttataat actgtaatgc agtaaaatag catgttgtag
    18601 ctatactaca gcaaattaac ataccattgt agtatattaa ttggaataat tttaagtact
    18661 tcaaaattcc agcaaaacat atatgtgttt ttgatattat gtatattcgt taatagaata
    18721 gctatacttg ttttacataa taaattatat gatacttgct atatgtaatc actatattta
    18781 atacacataa ctattatact tgctatatgt aattactata ctttatacac atagttatca
    18841 tatatgctat atgtaattac tatactttat acacatagtt atcatatata ctatatataa
    18901 ttactatact tggacacata gttatcatac atgctatatg taattactac acttggacac
    18961 ataactacta tattt
//
LOCUS       DAMJVK010000076        18897 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.077, whole genome shotgun sequence.
ACCESSION   DAMJVK010000076 DAMJVK010000000
VERSION     DAMJVK010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18897
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.077"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(208..1785)
                     /locus_tag="PC414_002712"
     CDS             complement(208..1785)
                     /locus_tag="PC414_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp5"
                     /protein_id="HDF2465100.1"
                     /translation="MKAPKTILTILTIALTLSSISIIPSYALTEEKLIGNGRYETAVK
                     ISQKAYSSSPNVVLVNDNSLADALSATPFAKAKGAPILLTESDKLDDRTEKEIKRLGA
                     KDIYLIGGTAVLNKDIENKLKSNGLNVERINGKDRYETSLILANKLKDIKDIKEVAVV
                     NGEKGLSDAVSVGAPAAQNKMPIILSSPKDGVEAFDKFIRDEKVIKAYVIGGTNSVSR
                     AVEKSLPNAERVSGKDRNETNAKVIEKFYTDTNLSNLYVTKDGSKNENQLIDSLAVGV
                     LAAKNESPVVLVGNKLNTKQRDILSTKKLNTITQVGGNGNEEAFDEIKSLQEKTVFEA
                     KTVEELTDMINIASPNDIINFKPKENTVNEAFRMVTNKPITVNIKGDCSKTLTVDMPN
                     GEVNNYATLVNVIVRNIGEGGFNNHDTITILSVRDKNGRVIENTRNSDIDTLMILASA
                     NDTKLINDGYIGKLIDNSSNSDITNNGTIDKKVNQVEDLEAKVDSIEKAIDSISQKVN
                     KIQDILDKLGFLKKFLS"
     gene            complement(2082..4493)
                     /locus_tag="PC414_002713"
     CDS             complement(2082..4493)
                     /locus_tag="PC414_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HDF2465101.1"
                     /translation="MRKYKLKKLSKLLVLLTVCFLIVSTIPVSAEKNKILTGVETAEY
                     SESYLQYLEDVKNGDIAKYNGVIPTPYEMEGTTLKTNVRSSLPASYKSSVSYDPRKLD
                     LTTPAKDQGKLNTCWAFSGMSTLEAYLKLKGYGTYDLSEEHFRWWSTGGIHGWNLTDM
                     TGSSNVTAIGYLTAWAGPKLEKDIPYNFKSEDEGATRPQNMDTAPTQFNVTDVVRISK
                     DKTSVKNAIMQYGAVTSGYAHYSAYLSDDENSYNCNDKSEPLNHSVSIVGWDDNYSKD
                     KFKPSVRPESNGAWLVKSSWGEFNSEKGFFWISYEDKTLLKDTDNYAMKSVSKPDSDK
                     KMYQLEYAGLSKIMSNKVTAANVFDFSRDSEKLDSVMFETDSVGAKYEVYYAPVVNGV
                     PQNNSMTKLASGTVSYSGYINVPTNSYSLPKGKGAIVVVIDNTANPNREKSTLAYETD
                     IDGYYLYEAKANLGESYILQNNKFEDINTYSEFSPCNFVIKAITKTSSGQATSGESLT
                     GADRYETAVKVSQKGWTSSQNAVLVNGDAIVDALTATPFTAAIDSPILLTGKDNLDSK
                     TKAELQRLGTKKVYLIGGENSLSKNVQTQLSNMGISVERISGSDRYKTSISLAQKLNS
                     IKSVSQVAVANGVNGLADAISVGAAAADNNMPIILTNEKSELQGADEFLNSSKITKSY
                     IIGGTATLSSNLESKLSNPTRLAGSNRNETNAKIIDKFYPSSDLKYAFVVKDGSKSQG
                     DLIDGLAVGALGAKTDSPVVLVGNKLDESQKNVLKSKKIETPIRVGGNGNENAFNELN
                     TLLGK"
     gene            4683..5084
                     /locus_tag="PC414_002714"
     CDS             4683..5084
                     /locus_tag="PC414_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HDF2465102.1"
                     /translation="MILKKHLETILYLFFGALTVLINIITYLFFTRIINFTFLLSNSL
                     SWSIAVLFSYITNKFFVFKSEKTEFLFLLKEFVSFLSCRLFSGFIEILLMYLMIDLFS
                     LNDILVKIGTNIIVIILNFVFSKVVVFKHRN"
     gene            complement(5149..7266)
                     /locus_tag="PC414_002715"
     CDS             complement(5149..7266)
                     /locus_tag="PC414_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HDF2465103.1"
                     /translation="MQISKKIISLLTAAFLSIVLPSNVVNASNKDSLIGSGRWETAIK
                     ISQNGWLRSDNAVLVNDNSIADALSATPFAKLKDAPILLSGSKELDFRTKSELKRLGV
                     KNVFLVGGSNALGVNVENELKSEKITVERIFGNDRYETSLKLAEKLNNEKDISDIIVV
                     NGEKGLADAVSVGSIAAQNYMPIILSDSNLGVEVANKFIKAQNIKNSYIIGGLNSISN
                     EIEKNLPNSKRISGNNRNETNANVIKEFYKETDLRNAYITKDGMKKQSDLIDSLSVGV
                     LAAKNNSPVVLVGNKIDSIQKDVLNTKIFSTVTQVGGIGNENVYKDILDMQEETKYTV
                     ETIEELDSALKKVDANDIIKFRPDKKISESFKLETEKAIKVDLYGEYSQSITINMPNG
                     ELNNTGDISKTLILKDIKDGTLTNSGYINQMDISDDNGCKIENENNGQIWIVTIFSSA
                     KDVNIRNDGTITKIDNDSSSTNIRNSGKIDTVSGNKEPGISGNKPNTNNTDKDDDSDT
                     AAGLSPKISACSPLENNQIMLTVPQKPKNSNYKIYYRVLSYKPSAMRVGDRININDWD
                     LVEDTEPLKVNAKNGSYIEVVEIDYTRKKVSRWGRAGATDDGFKIVANGIKSTVSVDE
                     NSKVTISVTGYNGNKVYYRIKTTKPEAMNVGDEIDSSWKSTSNSDIILEGNKVNGLYV
                     ELASGENNKVEKWGATDKIEIVS"
     gene            complement(7297..8004)
                     /locus_tag="PC414_002716"
     CDS             complement(7297..8004)
                     /locus_tag="PC414_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HDF2465104.1"
                     /translation="MVDATLFFTPHQDDETLSMGSAIIEHVEKSDTHVILCTDGSKSI
                     IRKVLDDGGSCSYHIKDVHKYSLSESEFSKDRDEEFKDSCEAMGVKESNIHIEDNRAH
                     DGELSKEKAKEIILKYLEEYPEAKVKTVTPFKASGIHEDHRALGEAALELYREGKIKD
                     LRFYVEPYDYKDFKKVNPNVEVWKVLPSQEEKLLSAMNAYKKWNPESGHYAIGYHSVK
                     SHFDDLELNKTQYVHAP"
     gene            complement(8111..9982)
                     /locus_tag="PC414_002717"
     CDS             complement(8111..9982)
                     /locus_tag="PC414_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp2"
                     /protein_id="HDF2465105.1"
                     /translation="MNKKNLSVIMAAAMISTSVAPVFAAETTQVKKETITKKEATELV
                     SKVRGLMSQKYTGGSQVGQPIYEIKVGETLSKLKIITNIDELEKLVNALGENKELIVT
                     ITDKGHITNSANEVVAEATEKYENSADLSAEANSITEKAKTETNGIYKVADVKASYDS
                     AKDKLVITLRDKTETVTSKTIYVGIGDEKVDLTANPVDSTGTNLDPSAEGFRVNKIDK
                     LGVAGAKNIDDVQLAEITIKNSDLNTVSPQDLYDGYRLTVKGNMVANGTSKSISDISA
                     KDSETGKYKFTIKYTDASGKATELTVESTNEKDLKDAKAALEGNSKVKLIAGDDRYAT
                     AVAIAKQTKYTDNIVIVNSNKLVDGLAATPLAQSKKAPILLASDNEIPKVTLDYIKDI
                     IKKSPSAKIYIVGGESAVSNTAKKQLESVTKNVERLAGDDRHMTSVAVAKAMGSFKDA
                     FVVGAKGEADAMSIAAKAAELKAPIIVNGWNDLSADAIKLMDGKEIGIVGGSNNVSSQ
                     IENQLADIDKDRKVQRVEGETRHDTNAKVIETYYGKLDKLYIAKDGYGNNGMLVDALA
                     AGPLAAGKGPILLAKTDITDSQKNALSKKLNLGAEVTQIGNGVELTVIQKIAKILGW"
     gene            complement(10326..12671)
                     /gene="secA"
                     /locus_tag="PC414_002718"
     CDS             complement(10326..12671)
                     /gene="secA"
                     /locus_tag="PC414_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDF2465106.1"
                     /translation="MSVLDTILDKADEQEIRRLNVIVDKIESLEKDIENMSDDELKKM
                     TDLFRDRLKKGETLDSLLPEAFAVAREASKRVLGMRQYRVQLIGGIVIHQGKIAEMKT
                     GEGKTLVEVAPVYLNALTGKGVHVVTVNDYLAQRDKELMQPVYEFLGMSVGVIIADLI
                     PEVRKVQYNCDITYGTNNEFGFDYLKDNMVTKKFDKVQRELNFAIVDEVDSILIDEAR
                     TPLIIAGEGDEDSKLYELANSFIKTIKEEDYELDRKDKTVALKESGIDKAESFFGIKN
                     LTDIENIELYHHINQALRGHKLMEKDVDYVVVNQEVMIVDEFTGRVMDGRRYTDGLHQ
                     AIEAKEGVEIKDESKTMATITFQNFFRLYNKLSGMTGTAKTEEGEFESIYKLNVVQIP
                     TNKPIIRADLHDKVFRTEKEKYQAVVEEVIRIHKTKQPILIGTVSVEKSEMLSGMLKK
                     EGIKHQVLNAKENDKEALIVSKAGKLGSVTIATNMAGRGTDIALGAGDKEEESRVKEL
                     GGLYVIGTERHESRRIDNQLRGRSGRQGDPGTSRFFVSLEDDVIKLYGGKSIEKLMKR
                     PNSNKNNSIESKSLTKAIERAQKGVEGKNFELRKNVLKYDDTINEQRKVIYNERNKVL
                     NDEDIQVDIQNMVKDIIKEAAAEYLTKRKRDYYGYFKHLYNTFMPADTLLIPDLDNKS
                     IEDIIDSTYEISKRVYDLKKMVVGLDKITEMEKIVLLKVVDQYWVEHIDAMEQLKQYV
                     GFKSYAQKDPFKEYAMEGYEMFESLNKNIRETTIQYLYKFN"
     gene            complement(12907..15087)
                     /gene="slpA"
                     /locus_tag="PC414_002719"
     CDS             complement(12907..15087)
                     /gene="slpA"
                     /locus_tag="PC414_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein SlpA"
                     /protein_id="HDF2465107.1"
                     /translation="MNKKNLAVVMSAVTVIGSAAPVFAAAEDSFPNGNTTVSSSKYSD
                     IERILKKYVADGVTGITVNFFDKKGNPKGVSISSLEASQISYVKDEIKDLKAGEYAKI
                     SVKITDGTEKKYDDTLLKELDSNAVVTKVGTATTVKIVDQDGNSIENDAKLKLQKYDT
                     TTSAYVDGATKDEVLAATKAAVKTSKFTDKTGNNATGTVAITATYADAVAGAIKLKYD
                     TQAAVAPTLDTAVASLDGTKEIYDFSKPVITVNTTDGTSKLSFEKTGEKVGAVESNVT
                     VIAASDETVTISGDAKEKAEALAKKYVFKDTELEDAYKTVTASDFEKTDNDYYEVVLY
                     PTGKRLNTASTYASSNYKPELPTSDRVDTPAIITLRSTNKNNLKSALDELRTYNNSYS
                     NNSVLAGDDRIETAIEISKDSYNADGGIKGDYVEANEVVLVGSQSIVDGLVASPLAAV
                     KDAPLLLTSKDKLDSSVKSEIKRVMGLDDKTGITSKKTVYIAGGENSVSKEVANELKD
                     MGLKVERLSGDDRYATSLEIADEIGLNHNKVFVVGGTGLADAMSIASVASNKEMPIVV
                     VDGKGKDLSTDAKDFIGSAYVDIIGGKSSVSEDMEDAIDDATGKSPERVSGDDRQDTN
                     AEVIKTYFEKDNSDSVISTGVKNFYVAKDGSTKEDQLVDALAIAAVAGHNEAPIVLAT
                     DSLSSDQSVAISKVTNSDDSKKLTQVGKGIADSVIKRIKDLLEL"
     gene            complement(15380..16978)
                     /locus_tag="PC414_002720"
     CDS             complement(15380..16978)
                     /locus_tag="PC414_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HDF2465108.1"
                     /translation="MKVNKRVLSIGLTISLIMAGAPNINALSSIEKIQGKDRYETSAL
                     IADKQIYDTVILVNTDNSIVDGLSASGLSGVVKAPIMLVQRDKIPTDVEKRLKDVKNA
                     YVIGTEDTIGKSVENQLKNKGIEVKRIGGEDRIKTSYLIAKEISAIKPVNDGDKVFLV
                     NGYTGEADAMSVSSVAARDGVPVILTDGKSIPFKVDGVQCYSLGSEEIMSNELVSKTN
                     SVRIAGKDRFETNKKVIQRFYKGTKKFYVSQGYKLVDAVAGSPLAKDKPIVLVNDGSD
                     KSVLRGADEVTSLGGMDKKVADQCISSASDKNTMPTITANDVEISVGDKFDNSMLNIV
                     ATDYYGNNLKPNIEGTVDTSKAGTYVLNVSAFDNFGQKCEINVNVKVIINTSTKDPNS
                     YEFKAMVSNEMYNLVNSYRKEKGKKSLRELDSLAGMANAWSKYMDEKKIFAHEIDGKN
                     AAEVFSGFGMRTDENIAYLPMNVKSVYTSKDAKEIAKSVFDLWKKSSKYNDNMLKEEF
                     YSFGFGMYVSNQREVNATMEFLNS"
     gene            complement(17197..18798)
                     /locus_tag="PC414_002721"
     CDS             complement(17197..18798)
                     /locus_tag="PC414_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp11"
                     /protein_id="HDF2465109.1"
                     /translation="MKMNKKILSLGLAVSLILVNFKSVNASSVVEKIYGKDRYETAAK
                     IADKQTYETVILVNTEKSLADGLSASGLSGATKAPILFTQQNKIPADTNRCLKNIKKA
                     YIIGTEDTISKSVEKELDSKNIEVKRIGGEDRLKTSYLIAKEIATIKKVDKVLLTNAY
                     SGEADAMSVSSVATRDGAPIILTDGKSVPFDVKNIQSYCIGSEEIMSNPLVKNTNSVR
                     IEGTDRFETNKNVIDYFFNSADGFYVSDGYQLVDAIAAAPLTKNSPMVLVNDGSDKTV
                     LEGAKNITSVGEIDEKVIQQCISASKSNGQPPAITVGSTEVYKGEKFDTGKLNIVAKD
                     NTGKVLPIEVDGFIDTNRVGTYILTLKATDEWGKSTGKRVEIKVLDDKSHDYNSPEFK
                     KMVSTEMYNLINSYRKEKGKEPLVVSSKLEGMANAWSKYMMDNKVFEHYIDGKNAPQV
                     FSEFGMRSEENIAYIYIDSKSVQTTQDAKDLAKAIFEVWKKSPEYNANMLSDEFYSTG
                     FGLYILSDGQVHATQEFLNGNEGSL"
BASE COUNT         5562 a         3554 c         2016 g         7765 t
ORIGIN      
        1 agattatttt tctttattct agttttgtca atacttttat ttattttttt gttacattca
       61 attccaatcc ccatgttacc tccccaaagc ttattcaaat atatactaaa ctacatattt
      121 atctataaat ctatttgtat ataaacctgt ttaactacat aactttagaa caattacaac
      181 aaacacttca cttaaatata tatattctta gctaagaaat ttctttagaa atccaagctt
      241 atctagtata tcttgtattt tattgacttt ttgacttata gaatctatag ctttttcaat
      301 tgaatctact ttagcctcta aatcttcaac ctgattaact tttttatcta tagtaccatt
      361 attagttata tcactattag atgaattatc tataagtttt cctatatatc catcattaat
      421 caattttgta tcattagcag aagctaatat catcaacgta tctatatctg aatttctagt
      481 attttctata actctaccat ttttgtcacg aactgacaat atagtgattg tatcatggtt
      541 attaaatcca ccttctccaa tatttctaac tataacattt accaaagtag catagttatt
      601 cacttcacca ttaggcatat ctactgtaag agtctttgaa caatcaccct ttatattcac
      661 agttattggt ttatttgtta ccattctaaa agcttcattt acagtattct cttttggttt
      721 aaagtttata atatcattag gacttgctat atttatcata tcagttaatt cctcaacagt
      781 ttttgcttca aacacagttt tttcttgtag acttttaatt tcatcaaaag cttcttcatt
      841 tccatttcca cctacctgtg ttatagtatt taatttttta gtactaagta tgtccctttg
      901 ttttgtgttt aatttattac caaccaaaac aacaggagac tcattttttg cagccaatac
      961 tccaacagct aacgagtcta ttagttgatt ctcattttta ctaccatcct tagttacata
     1021 taaattactt aaatttgtat cagtgtaaaa cttttctatg accttagcat tagtctcatt
     1081 tctatctttc ccactcactc tttctgcatt aggaagactc ttttctacag ctcttgaaac
     1141 agaatttgta cctccaatta catatgcttt tataactttc tcatccctta taaatttatc
     1201 aaatgcttct actccgtcct taggactaga caaaattata ggcatcttat tttgagctgc
     1261 tggtgcccca acgctaacag catcactcaa tcctttttca ccatttacta cagcaacttc
     1321 cttaatatct tttatatcct taagcttatt agccaaaatc agtgaagttt catatctatc
     1381 cttaccatta attctttcta cattaagtcc attacttttt aacttgttct ctatatcttt
     1441 attaagtaca gcagtacctc ctatcaaata aatatcttta gctcctagac gttttatctc
     1501 tttttcagtc ctatcatcta gcttatcact ttctgtcaaa agtattggtg ctcctttagc
     1561 ctttgcaaat ggtgtagctg aaagtgcatc agctaatgaa ttgtcattaa ctagcactac
     1621 attaggacta gaactgtatg ccttttgact tatctttaca gcagtttcat atctaccatt
     1681 acctattaat ttttcctctg taagtgcata tgatggtatt atagaaatgc tacttaaagt
     1741 aagcgctatt gtcagtattg ttaaaatagt ttttggtgct ttcattatta ttccccctta
     1801 ttttgtttaa aatcttacta tatttatatt ataaaataac tttacactat tttacagttt
     1861 aaaattatta catttttcat caataataag aaaactatct aaaaatatag acaatcctat
     1921 ttttcattat ttaaatgata aaaatataaa acaacaaaaa agagtgcctc atgaactaaa
     1981 aagttcattt tgagacaccc cgttttgtta ttttatattt ataaacttct attgaatatt
     2041 ttttaataat aaaatgtata tccttactat ctttccattc actattttcc taaaagagta
     2101 tttagttcat taaaagcatt ttcatttcca tttccaccaa ctctaatagg agtttctatt
     2161 ttcttagact taagtacatt tttttgactt tcatctaact tatttccaac taaaactact
     2221 ggtgaatcag ttttagcacc taatgctcct actgctagtc catctatcaa gtctccttga
     2281 ctctttgaac catctttaac aacaaaagca tatttcaaat ctgagctagg atagaattta
     2341 tctattatct tagcattagt ttcatttcta ttacttcctg caagtcttgt tggatttgaa
     2401 agcttacttt ctaaatttga tgataaagta gctgttccac caattatata agacttagtt
     2461 attttagatg aatttaaaaa ctcatcagca ccttgcaact cactcttttc attagtaagt
     2521 attattggca tattgttatc agcagctgct gcaccaacac ttattgcatc tgcaagtcca
     2581 tttacaccat ttgccactgc aacttgtgaa acagatttta tactatttaa cttttgagct
     2641 agagatatac tggtcttgta tctatcacta cctgaaattc tttctactga tatacccata
     2701 ttactaagtt gagtttgtac attcttgctt aaagaatttt ctccacctat tagataaact
     2761 tttttagttc ctaatctttg taactctgcc ttagtttttg aatctaagtt atcttttcct
     2821 gtcaaaagaa ttggagagtc aattgctgct gtaaatggtg tagctgttaa agcatccact
     2881 attgcatctc catttactaa tactgcattt tgtgaagaag tccatccttt ttgactaact
     2941 ttaactgctg tttcatatct atctgctcca gttaaagatt ctcctgaagt agcttgtcca
     3001 gaagatgttt ttgttatagc ttttataaca aagttacaag gagaaaattc actatatgta
     3061 tttatatctt caaatttatt gttttgaagt atgtagcttt cacctaagtt tgctttagcc
     3121 tcatatagat agtatccatc tatatcagtt tcatatgcta aagttgattt ttctctatta
     3181 ggatttgctg tgttgtctat aactactact attgctcctt taccttttgg taagctgtaa
     3241 gaattagtag gtacatttat gtatccagaa tatgatactg ttccacttgc aagttttgtc
     3301 attgaattgt tttgaggaac tccatttact actggtgcat aatatacttc atattttgct
     3361 cctacagaat ctgtttcaaa cataacagag tcaagttttt cagaatctct gctaaaatca
     3421 aatacatttg ctgctgttac tttatttgac attatcttgc taagaccagc atattcaagt
     3481 tggtacattt ttttatcgct atctggtttt gatactgatt tcattgcgta gttatctgta
     3541 tcttttaaaa gagttttgtc ttcataagaa atccagaaaa agcctttttc tgagttaaat
     3601 tctccccaac tactttttac taaccatgct ccatttgatt ctggtctaac acttggtttg
     3661 aacttatctt ttgaataatt atcatcccat cctacaattg aaacagaatg atttaaaggt
     3721 tcacttttat cattacaatt atacgaattt tcatcatcac ttaagtaggc tgaatagtgt
     3781 gcatatcctg atgtcactgc accatattgc attattgcat ttttaacaga agttttatct
     3841 ttactaattc ttacaacatc tgttacatta aattgtgtag gtgcagtatc catattttga
     3901 ggcctagttg ctccttcatc ttctgattta aaattatatg gaatgtcttt ttccagctta
     3961 ggtcctgccc aggctgtaag atatccaatt gcagttacat tcgaactacc tgtcatatca
     4021 gttaagttcc atccatgtat tcctccagtt gaccaccatc taaaatgctc ttctgaaaga
     4081 tcataagttc cataaccttt taatttcaaa tatgcttcta gtgttgacat tccagaaaaa
     4141 gcccaacatg tattaagttt tccttggtct tttgctggtg tagttaaatc taattttctt
     4201 ggatcatatg atacacttga tttatatgat gctggaagac tacttcttac attagtttta
     4261 agtgttgttc cttccatttc ataaggagtt ggtattactc cattatattt tgctatatct
     4321 ccatttttta catcttccaa gtattgaaga tatgattcag aatactctgc agtttctact
     4381 ccagtcagaa ttttattttt ttctgctgag acaggtattg ttgacacaat taaaaaacaa
     4441 actgtcaaca acaccagtag ctttgacaat ttttttaatt tatactttct catatttacc
     4501 cccttgtatg tatttaattt ttattatatc agatttctaa atctaggtca tattttattt
     4561 ttataaaaat aggaattata attatacata tataaaatac tattttatac tgcatattta
     4621 ttgattaata tgtggtatac taattacata ataatatata ccaaataatg gaggaaaatt
     4681 ttttgatatt aaaaaaacat ctagaaacaa tattatatct tttttttggt gctttaacag
     4741 ttttaataaa cattattact tacctatttt ttacaagaat tattaatttt acttttttat
     4801 tatcaaactc tctatcttgg tctatagcag ttttattttc atatataaca aacaaatttt
     4861 ttgtattcaa aagtgaaaaa actgagtttt tatttctatt aaaagaattt gtatcttttt
     4921 tatcttgtcg tttattttct ggttttatag aaatattact tatgtattta atgattgatt
     4981 tattttcttt aaatgatatc ttagtaaaaa taggcactaa tataattgtg ataattttaa
     5041 acttcgtctt cagcaaggta gtagttttta agcatagaaa ttaacattat tatatttatc
     5101 tttatgcaga ataaggagtt gcctaaaaaa aacaactcct tattagcttt aagaaacaat
     5161 ttctatttta tctgttgctc cccatttttc aactttatta ttttctcctg atgctaattc
     5221 tacataaagt ccatttacct tatttccttc taaaattata tcactattag aagtactttt
     5281 ccaactggag tctatttcat ctcctacatt catagcttct ggttttgtag tctttattct
     5341 atagtaaact ttattaccat tgtatccagt tacactaata gttactttag aattttcatc
     5401 aacagaaaca gttgatttta ttccatttgc tactatttta aaaccatcat ctgtcgcacc
     5461 agctcttccc catcttgaaa ctttctttct tgtataatca atttcaacaa cttctatata
     5521 tgatccattc ttagcattta cttttaatgg ttccgtatct tcaaccaagt cccaatcatt
     5581 tatgttaatt ctatcaccaa ccctcatagc tgatggttta tatgacaaaa ctctataata
     5641 aattttataa tttgaatttt taggtttctg aggtacagtt aacataattt gattattctc
     5701 aagaggagaa catgcactta tctttggaga taatcctgct gctgtatcac tatcatcatc
     5761 tttatctgta ttattagtgt taggtttatt gccacttatt cctggttctt tatttccact
     5821 aacagtatca atctttcccg aatttcttat attagtactt gaagaatcat tatctatctt
     5881 tgttatagtt ccatcatttc ttatatttac atcttttgca gaactaaata ttgttacaat
     5941 ccatatttgt ccattatttt cattttcaat cttgcaacca ttatcatcag aaatgtccat
     6001 ttgatttata tatcctgaat tagttaaagt tccatctttt atatctttaa gtatcaatgt
     6061 tttacttata tcacctgtat tattcaactc tccatttggc atatttatag ttattgactg
     6121 actatattct ccatataaat caacctttat agctttttca gtctctaatt taaaagattc
     6181 acttattttt ttatcaggtc taaactttat aatatcatta gcatccactt tctttaaagc
     6241 agaatccaat tcttcaattg tttcaactgt atatttagtt tcttcttgca tatccaatat
     6301 atctttatag acattttcat ttcctattcc accaacctga gtaactgtac taaatatctt
     6361 agtatttagt acatctttct gaatagaatc aattttattt cctactaata ccactggaga
     6421 attgtttttt gcagccaaca ctccaactga tagtgaatct attaaatcac tttgtttttt
     6481 cattccatcc ttggttatat atgcatttct taaatctgtt tctttataaa attcttttat
     6541 aacattagca tttgtttcat ttctattatt tcctgatatc ctcttagaat ttggtaaatt
     6601 tttttctatt tcattagaaa tggaatttaa tcctccaata atataagaat tttttatatt
     6661 ttgggctttt ataaatttat tagctacttc aactccaagg tttgaatctg aaagtataat
     6721 tggcatgtaa ttttgagctg ctattgaacc aacacttaca gcatctgcta atcccttttc
     6781 tccatttact acaattatat cagaaatatc tttttcatta ttcaattttt cagccaattt
     6841 taaggatgtt tcatatctat cattcccaaa aattctttca actgttattt tctctgattt
     6901 taattcattt tctacattta cacctagtgc atttgaacct cctactaaaa atacattttt
     6961 tactccaagc ctttttagtt ctgattttgt cctaaaatcc aattctttac ttccagatag
     7021 taaaataggt gcatctttca atttagcaaa tggagttgca gataatgcat ctgctattga
     7081 gttatcattc actaatacag cattatcaga tcttaaccaa ccgttttggc ttatttttat
     7141 tgctgtttcc catctacctg aacctattaa actgtcttta ttagatgcat ttaccacatt
     7201 actaggtaaa acaattgata aaaatgctgc tgttaataaa gatattattt tttttgatat
     7261 ttgcattaat tatctcctcc taatttaaat ataattttat ggagcatgta catactgtgt
     7321 tttatttaat tctaaatcat caaaatgaga ttttactgaa tggtatccta tagcataatg
     7381 tccactttca ggattccatt tcttatatgc attcatcgca cttaataact tctcctcttg
     7441 actaggtaat accttccaga cttctacatt tggattaact tttttaaaat ccttatagtc
     7501 atatggttct acataaaatc ttaaatcttt tatttttcct tctctatata actctagtgc
     7561 agcttctcct aatgctctat gatcttcatg tattccacta gctttaaacg gagtaacagt
     7621 ttttactttt gcttctggat attcttctaa gtatttaagt attatttcct ttgccttttc
     7681 tttgcttaac tcaccatcat gagctctatt atcttctata tgtatattac tttcttttac
     7741 acccattgct tcacagctat ccttaaattc ttcatctcta tcttttgaaa attcactttc
     7801 tgataaagag tatttatgaa catctttaat atgataagaa catgaccctc catcatccaa
     7861 aacttttctt attatagatt tactaccatc tgtgcacaat atgacatgtg tatcactttt
     7921 ttcaacatgt tcaattatag cacttcccat acttaaggtt tcatcatctt gatgtggtgt
     7981 aaaaaacaaa gttgcatcta ccactactac atcctcctac tttataaaat gttataattt
     8041 acaataaata gcataattat agaaaaagat tcctgtatta attaaggaat cttctccaca
     8101 gtataagtta ttaccaacct agtattttag ctatcttttg tattactgtc aattcaactc
     8161 cattacctat ttgagttact tctgcaccaa gatttaattt tttactaagt gcattctttt
     8221 gtgagtctgt tatatcagtt ttagctagaa gtattggacc tttacctgct gctagaggtc
     8281 ctgctgccaa tgcatctact agcataccat tatttccata tccatctttt gctatatata
     8341 gtttatctaa tttgccataa tatgtttcta taactttagc attagtatcg tgtcttgttt
     8401 ctccttcaac tctttgaact tttctatctt tatcaatatc tgcaagttga ttttcaattt
     8461 ggctagatac attattagag ccaccaacta tgccaatctc ttttccatcc atcaatttga
     8521 tagcatctgc tgatagatca ttccaaccat ttactattat aggagcctta agttcagcag
     8581 ctttagcagc tatactcata gcatcagcct ccccttttgc gcctactaca aatgcatctt
     8641 taaaagaccc catagctttt gctactgcta cagaagtcat atgtctatca tctccagcta
     8701 gtctttcaac attcttagtt actgattcta attgcttttt agctgtattt gatactgctg
     8761 attctccacc tactatatat attttagctg atgggctttt cttaattata tcttttatat
     8821 aatctaaagt tacttttggt atttcattat cagatgctaa taatataggt gcttttttag
     8881 attgagcaag tggtgtagct gctaatccgt caactagttt atttgaatta actataacta
     8941 tattgtcagt atattttgtt tgttttgcta tagccactgc agttgcatat ctatcatctc
     9001 cagctatcaa tttaaccttt gaattacctt ctaatgcagc tttggcatct tttaaatctt
     9061 tttcattggt actttctaca gtaagctctg ttgcttttcc agatgcatca gtatacttaa
     9121 tagtaaattt atattttcct gtttctgaat cttttgctga aatatcacta attgactttg
     9181 atgtaccatt tgctaccata ttacctttaa cagtcaatct atatccatca tataaatctt
     9241 gtggtgaaac tgtatttagg tcactatttt ttatagttat ttcggccaat tggacatcat
     9301 caatattttt agctcctgct acacctagtt tatcgatttt atttactcta aatccttctg
     9361 cagaagggtc taaatttgtt ccagttgaat caactggatt tgctgttaaa tcaacttttt
     9421 catcaccaat acctacatat atagttttag aagttactgt ttctgtttta tctcttaaag
     9481 ttataactaa cttatcttta gcactatcat atgaagcttt tacatctgca actttataaa
     9541 ttccattggt ttcagtttta gctttttctg ttatagaatt agcctcagcg gaaaggtctg
     9601 ctgaattttc atatttttca gttgcttctg caactacttc atttgcacta tttgttatat
     9661 gtcctttatc tgttatagtt acaataagtt ctttattttc tcccaaagca tttactaatt
     9721 tctctaattc atctatatta gttattattt ttaattttga taaagtctca ccaactttta
     9781 tttcatatat tggttgtcca acttgagaac ctcctgtata cttttgagac attaaacctc
     9841 taactttcga aactaattct gtagcctctt tcttagttat tgtttctttt tttacctgtg
     9901 tagtttctgc agcaaaaact ggagctactg atgtacttat cattgcagca gccataatta
     9961 cagaaagatt ttttttattc atggtatttt ccccctaaaa aattcaaatt tttattattt
    10021 tttccttatt taccaattat aatatatatt tgatattatt tcaataaatg agacttattg
    10081 taataattaa ccaaaagata taaacttttt cttatatttt atttttttaa tatttacgta
    10141 tacaaaaaag taacaaaatt gtaatttttt ttaataatcc agtttataac taatagactt
    10201 ttcatcttct ttatagtata atattaaatg gaagaaagaa atttcaagca tctctttttt
    10261 ccgtatttct cacatagaaa aagaggctga ttatcagcct ctttttccta tacagtttaa
    10321 atttcttaat taaatttata taaatactgt atagtcgtct ctcttatatt tttatttaat
    10381 gattcaaaca tctcataacc ttccatagca tattctttga aaggatcttt ttgagcatat
    10441 gatttaaatc ctacatattg ttttaattgc tccatagcat ctatatgttc tacccaatat
    10501 tgatcaacta cttttaaaag aacaattttt tccatttcgg ttattttgtc aaggcctact
    10561 accatctttt ttaaatcata aactcttttt gatatttcat aagtactatc aattatatcc
    10621 tcaatacttt tattatctaa gtcaggtatt agtaaggtat cagctggcat aaatgtatta
    10681 taaagatgct taaaatatcc atagtaatcc ctctttcttt ttgttaaata ttcagcagca
    10741 gcttctttta ttatatcttt aaccatgttt tgaatatcta cttgtatatc ttcatcattt
    10801 agaactttat ttctttcgtt atagattact tttctttgtt catttatagt atcatcatat
    10861 ttaagaacat ttttccttaa ttcaaaattt ttaccttcca cccctttttg agctctctct
    10921 attgcctttg taagagattt actttcaata gaattatttt tatttgaatt tggtcgtttc
    10981 attagtttct ctatactttt tccaccatac aatttgatta catcatcttc aagactaaca
    11041 aaaaaccttg atgttcctgg atcaccttga cgtccagaac gccctctaag ctgattatcg
    11101 attcttctag attcatgtct ctcagttcct attacataaa gtcctcctaa ttcctttact
    11161 ctactttctt cttctttatc acctgctcct aaagcaatat ctgttcctct acctgccata
    11221 ttagttgcaa tagttactga acccaactta ccagcctttg acactatcaa tgcttcttta
    11281 tcattttcct tagcatttag aacttgatgt tttattcctt ctttcttaag cataccagat
    11341 aacatttctg atttctcaac agaaacagta ccaataagaa ttggctgttt tgttttatgt
    11401 atccttatta cttcttctac aacagcttga tacttttctt tctctgttct aaacacttta
    11461 tcatgaagat ctgctcttat aattggtttg ttagtaggaa tttgtacaac atttaattta
    11521 taaattgatt caaattctcc ttcttcagtt ttagcagtcc ctgtcatacc agaaagttta
    11581 ttatataatc taaagaaatt ctggaatgta attgtagcca tagttttaga ttcatctttt
    11641 atttctaccc cttcttttgc ttctatagct tggtgtaagc catctgtata tcttcttcca
    11701 tccattactc ttcctgtaaa ttcatcaact atcattactt cttgattaac aacaacataa
    11761 tcaacatctt tttccataag tttgtgaccc ctaagagcct gatttatatg atgatataat
    11821 tctatatttt ctatatctgt aagattttta atcccaaaaa atgattctgc cttatcaata
    11881 ccactttcct ttagagctac ggtcttatct tttctatcta attcataatc ttcttctttt
    11941 attgttttta taaatgaatt tgctaattca taaagttttg aatcttcatc accctctcca
    12001 gcaattataa gtggagttct agcttcatct ataagaattg agtcaacctc atcaactatg
    12061 gcaaaattca actctctttg tactttatca aactttttag ttaccatatt atcttttaaa
    12121 taatcaaatc caaattcatt atttgtacca taagtaatat cacaattgta ttgaactttt
    12181 ctaacttcag gtataagatc tgcaataatt actcctacag acatacctaa aaactcatac
    12241 actggttgca ttagctcttt atcacgttgt gctagatagt catttactgt aacaacatgc
    12301 acacctttac ctgttaaagc atttagataa acaggtgcaa cttctaccaa agttttacct
    12361 tcaccagttt tcatttctgc tatttttcct tgatgaatta caatgcctcc aattaattga
    12421 accctatatt gacgcattcc taaaactctt tttgaagctt ctcttgccac tgcaaatgct
    12481 tctggtaata aactatctaa agtttctcct ttttttaatc tatctctaaa taggtctgtc
    12541 atttttttta attcgtcatc agacatattt tctatatctt tttctaaaga ttctatttta
    12601 tcaactatca cattaagcct tcttatttcc tgctcatctg ctttatccaa tattgtatcc
    12661 aaaactgaca tatttaattt acctcctttt ttatcataca attactatta taccttaaaa
    12721 ttatagaata gtagacaatt atttttattt atagtataat aatcatactt aaataaatta
    12781 catctttaat tatttaggta tttctcacaa aaaaaggaac tatttttagt tcctttttta
    12841 atttcttttt ataaaaaaga gaacttcttc aaagaagttc cctcttattt atagtttaat
    12901 tttatcttat aattctaata aatcttttat tctcttaata actgaatctg ctattccttt
    12961 accaacttga gtcaatttct tagaatcatc agaatttgta actttactta ttgctactga
    13021 ttggtcagaa gataaactat cagttgctaa aactattgga gcttcattgt gacctgctac
    13081 tgctgctatt gctaacgcat ctactaattg atcttctttt gtagaaccat ctttagcaac
    13141 atagaagttt ttaacacctg tgctaattac actgtcacta ttatcttttt caaaataagt
    13201 ttttataact tctgcattag tatcttgtct atcatctcca ctaactcttt ctggactctt
    13261 acctgtagca tcatctatag catcttccat gtcttcagaa acactagatt ttccacctat
    13321 tatatctaca taagcacttc ctataaagtc ttttgcatca gttgataaat cttttccttt
    13381 tccatcaact actactattg gcatttcttt attagaagca actgatgcta tactcattgc
    13441 atctgctaat ccagtaccac caactacaaa tactttatta tggtttaaac ctatttcatc
    13501 agcaatttct aaagatgtag catatctatc atcaccagat aatctttcaa cttttaatcc
    13561 catatctttt aattcatttg ctacttcttt agatacagaa ttttccccac cagctatata
    13621 aacagtcttt ttagatgtta taccagtttt atcatctaat cccattactc tctttatttc
    13681 agattttact gatgaatcta acttatcttt tgaagttaat aataatggag catctttaac
    13741 agcagctaaa ggtgaagcta ctagaccatc tactattgat tgtgatccta ctaatactac
    13801 ctcattagct tctacataat ctcctttaat tcctccatct gcattataag agtctttact
    13861 tatttctatt gcagtctcta ttctatcatc accagctaat acagaattat ttgaataaga
    13921 attattataa gttcttaatt catctaaagc tgattttaag ttattcttat tagtagatct
    13981 taatgttatt atagcaggag tatctactct atcacttgta ggtaattctg gcttatagtt
    14041 agatgatgca tatgttgaag ctgtatttaa tcttttacct gttggataaa gaactacctc
    14101 ataataatca ttgtcagttt tttcaaaatc acttgctgtt actgtcttat atgcatcttc
    14161 taattcagta tctttaaata catatttttt agctaaagct tctgcttttt cttttgcatc
    14221 tccagatatt gtaactgttt catctgatgc tgctataact gttacatttg attcaactgc
    14281 tccaactttt tctccagttt tttcaaaact taattttgat gtaccatcag tagtattaac
    14341 cgttataact ggtttactaa aatcatatat ctctttagta ccatcaagag atgctactgc
    14401 tgtatctaaa gtaggtgcta ctgctgcttg tgtatcatat tttaatttaa ttgcaccagc
    14461 tactgcatca gcatatgttg ctgtaatagc tactgttcct gtagcattat taccagtctt
    14521 atcagtaaat tttgaagttt tgactgctgc ctttgtagct gctaaaactt catctttagt
    14581 agctccatct acataagctg acgtagttgt atcatatttt tgtaacttta atttagcatc
    14641 attttctatt gaatttccat cttgatctac aatcttaact gttgttgcag taccaacttt
    14701 agtaactaca gcattactat caagttcctt taataatgta tcatcatatt ttttttctgt
    14761 tccatctgta atttttacag atatttttgc atattctcca gcttttaaat ctttaatttc
    14821 atctttaaca taacttattt gacttgcttc taaacttgat atagataccc cttttggatt
    14881 tccttttttg tcaaaaaagt taactgttat gcctgttaca ccatctgcaa catatttttt
    14941 aagtattctt tctatatcac tatacttact actagataca gtggtatttc catttggaaa
    15001 actatcctct gctgcagcga aaactggtgc tgctgatccg attactgtaa cggcagacat
    15061 tacaactgcc aaatttttct tattcattcc ttaaattcct cccaacatta tattaattac
    15121 atctctttaa ttatgtacat taaaattcca tgttcatata aaccaaatat ttctcacact
    15181 cttatgatag actatataaa atagaatttc aataatttat gcaaaattaa tagaattttc
    15241 tttcaacatt tttccccttt tttttgctat ttcatatttt ttaaattaaa ttttcaattt
    15301 taattttatt aatacattta tttatctgat ttaatcaata tatattaata ttttctacca
    15361 taaaatatta atatatactc taactattta aaaattccat tgtagcatta acttctcttt
    15421 gattagaaac atacattcca aagccaaaac tataaaactc ttcctttaac atattatcat
    15481 tatacttaga agacttcttc cataaatcaa atacagactt agcaatctct tttgcatctt
    15541 tagatgtata tacagactta acattcatag gtaaatatgc aatattttca tcagttctca
    15601 ttccaaagcc agaaaatact tcagctgcat ttttcccatc tatttcatga gcaaatatct
    15661 tcttctcatc catatactta gaccatgcat ttgccattcc agctaaagaa tctaattctc
    15721 ttaaactttt cttacctttt tcttttctat atgaattaac tagattatac atttcattag
    15781 ataccatagc cttaaactca taactattag ggtcttttgt acttgtgttt ataattactt
    15841 tgacatttac attaatctca cacttttgcc caaaattatc aaaagcactt acatttaaaa
    15901 catacgttcc tgccttactg gtatccacag ttccctcaat attaggcttc aaattatttc
    15961 cataataatc agtggcaact atattcaaca tactattatc aaacttatcc cctacagaaa
    16021 tctctacatc attagcagtg attgtaggca ttgtattttt atcagatgca gaactaatac
    16081 attggtcagc aaccttctta tccattccac ctaaagaagt aacttcatca gcacctctaa
    16141 gcacactctt atcacttcca tcattaacaa gtactatagg tttgtcctta gccaacggag
    16201 aaccagccac agcatcaact aacttatacc cttgagaaac ataaaacttc tttgttcctt
    16261 tataaaagcg ttgtataacc ttcttattag tttcaaatct atctttacca gcaatcctaa
    16321 cagaattagt cttactcaca agttcattac tcattatctc ctctgaccca agagaataac
    16381 attgaacgcc atcaacctta aatggtatac tctttccatc agtaagtata acaggaactc
    16441 catctctagc agctacagaa gatacactca ttgcatcagc ctcacctgtg tatccattta
    16501 caagaaaaac cttatcacca tcatttactg gtttaatagc tgaaatctcc ttagctataa
    16561 gatagctagt ctttattcta tcttccccac caattctttt aacttcaatc cctttattct
    16621 taagttgatt ttctacagat ttacctattg tatcctctgt acctataaca taagcattct
    16681 taacatcctt tagtcttttt tcaacatctg ttggtatctt atctctttgc actaacatta
    16741 taggagcttt tacaactcca gataatccac ttgcacttaa tccatctact atagaattat
    16801 cagtatttac taatatgact gtatcatata tttgtttatc tgctatcaat gcagaagtct
    16861 catatctatc tttcccttgt atcttttcta tactcgaaag tgcattgata tttggtgctc
    16921 ctgccattat taatgatatt gtcaatccta ttgataatac tctcttattt accttcatcg
    16981 aactcctcct actaattttt ttatataatt cagctaatca taaattacac cctcagtagt
    17041 gattccctca aaatttataa actactaaaa caaaaaacac ctaaaatacc ataacctgta
    17101 aagcaataat aaaacagatt acaaaaagta ctttagatgt gattttcatt ttataatatc
    17161 agtaaattaa caaaaaaaca agatttatgg aattttttac aagctacctt cattgccatt
    17221 taaaaactct tgagtagcat gcacttgacc atcagataat atataaagcc caaatccagt
    17281 actataaaac tcatcactaa gcatgtttgc attgtattct ggagatttct tccacacttc
    17341 aaatatagcc tttgccaagt cttttgcatc ttgagttgtt tgaacactct tagaatctat
    17401 ataaatatat gctatatttt cctcacttct cattccaaac tcagaaaata cttgtggagc
    17461 atttttgcca tctatataat gttcaaaaac tttgttatcc atcatatact tagaccatgc
    17521 atttgccata ccttctagct tactagatac taccaaaggc tctttacctt tttcttttct
    17581 ataggaattg attaagttat acatttcagt agataccatt tttttaaatt ctgggctgtt
    17641 gtaatcatga gatttatcat ccaacacttt tatttctact ctttttcctg tactttttcc
    17701 ccattcgtct gtagctttta atgtcaatat gtatgtacct actctatttg tatctataaa
    17761 cccatcaact tctattggta ataccttccc agtgttatct ttagctacta tatttaactt
    17821 gccagtgtca aacttttcac ccttatatac ctctgtactt ccaactgtaa ttgctggggg
    17881 ttgtccattt gactttgaag cacttataca ttgttgtatt accttctcat ctatttcacc
    17941 tacagaggtt atattcttag ctccttctaa tacagtttta tcgcttccat catttactag
    18001 taccattgga gagtttttag taagtggtgc agctgctatt gcatctacta attggtatcc
    18061 atctgataca tagaatccat ctgcactatt aaaaaagtag tctattacgt ttttgttagt
    18121 ttcaaatcgg tcagttcctt ctattcttac tgagtttgta tttttaacta gaggattact
    18181 cattatctcc tctgaaccta tacaatatga ttgaatattt tttacatcaa aaggcacact
    18241 ctttccatct gtaagtatga ttggagctcc atctctagta gctactgatg atacactcat
    18301 tgcatctgct tctccactat atgcattagt tagtagtact ttatcaactt tttttatagt
    18361 tgctatttct ttagctatta aatagcttgt ttttagtcta tcttctccac caattctttt
    18421 gacttctata tttttagaat ctagttcttt ttctactgat ttacttatag tatcttctgt
    18481 acctattata tatgcttttt tgatattttt tagacatcta tttgtgtctg ctggtatctt
    18541 attttgttgt gtaaatagta ttggagcttt tgtagctccc gataatccac ttgcacttaa
    18601 tccatctgca agtgattttt ctgtatttac taaaattact gtttcataag tctgtttatc
    18661 agctatcttt gctgctgttt catatctatc tttaccgtat attttttcta ctactgatga
    18721 tgcatttaca cttttgaagt ttactaagat taatgatact gctagaccta atgataatat
    18781 ttttttgttc attttcatat ccccctgatg atttttatat tttatattta tattgtgatt
    18841 attttccatt tattttctct ttattttcca tatattatag agtatatatg atatgaa
//
LOCUS       DAMJVK010000077        18684 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.078, whole genome shotgun sequence.
ACCESSION   DAMJVK010000077 DAMJVK010000000
VERSION     DAMJVK010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18684
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.078"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(166..1527)
                     /gene="rlmD"
                     /locus_tag="PC414_002722"
     CDS             complement(166..1527)
                     /gene="rlmD"
                     /locus_tag="PC414_002722"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDF2465110.1"
                     /translation="MSVKKKDVIEFEVDKMEFGGTSLSQVGDRVIHMKGGISGQKVRA
                     GVKKVRSKKAEVKMIELLENSPLETEETCKHFRECGGCTLLSVPYEKQLEIKEKQVMD
                     LFLKQDLFGFQFLGIEGSPENKYYRNKMEYTFGDEVKNGPLTLGLHKKGKHIDIQTVE
                     ECMLVDEDFSKILVSSVEFFNEKKLPYYRTMNHKGYLRHLVVRKGIHTNEIMVNIVTS
                     SQEDFDMYEFKDMLLGIDLKAELVGVLHTINDGLADAVQCDELRVLYGRDYIQEEILG
                     LKFKISPFSFFQTNTKGAEVLYSIARDFIGDYNDKVVFDLYSGTGSIGQVMAGAAKKV
                     YGIEIVEEAVVAANENAKLNGLTNCEFIAGDVAKVVKDLKDKPDLIIVDPPRPGIHKD
                     AIRDICGFGAKEIVYISCNPKSLVVDLVDFKGYGYEIKMVKCMDMFPNTPHCETVVLL
                     RRK"
     gene            complement(1554..2276)
                     /locus_tag="PC414_002723"
     CDS             complement(1554..2276)
                     /locus_tag="PC414_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDF2465111.1"
                     /translation="MAKKQRIDKILSNLGYGSRSEIKKYCKQGSVVVNGSEVSNPGTQ
                     VDTENDEILFNGEEVIYREYIYLMMNKPDGYISATTDKYDPTVLDLIDLSYLAFEPFP
                     VGRLDKDTEGLLVLTNDGKLSHRVLSPKKHVPKTYYAKIDGVVTEEDVEAFLEGVVLD
                     DGYKTMPSQLNILKSDDESEIELTIHEGKFHQVKRMFESVGKKVVYLKRLSMGNLKLD
                     ESLELGEYRELTDEEVKLIEER"
     gene            complement(2276..2605)
                     /locus_tag="PC414_002724"
     CDS             complement(2276..2605)
                     /locus_tag="PC414_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1904 domain-containing protein"
                     /protein_id="HDF2465112.1"
                     /translation="MPQIKIRGINENDICKISEKMINDLVEAVKCPRDYFEIECIKSV
                     AIRDGKIADVYPFVEVAWFDRGQEVQDIVARIITDSIRNNLDVESIDLAFTVFEKEKY
                     YENGEHF"
     gene            2935..3861
                     /gene="fba"
                     /locus_tag="PC414_002725"
     CDS             2935..3861
                     /gene="fba"
                     /locus_tag="PC414_002725"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HDF2465113.1"
                     /translation="MALITTKEMFKKAYEGGFAIGAFNISDLEQLQGVLKAAKAKNSY
                     VMIQASMSAVKYAGPHTLVEMVKAASDEIGVDVALHLDHGPNMDAIKTCIDAGFSSVM
                     IDGSHFDFEENVRITKEAVEYAHSKGVVVEAELGVLAGTEDDVTSDVHKYTQPAEAVE
                     FVERTGVDSLAIAIGTSHGAFKFKGEAKLRFDILEEIQSKLPGFPIVLHGASAVDQNA
                     VATCNEFGGNIAGAKGVPVDMLRKASSMAVCKINMDTDLRLAMTAAIRKFLAENPKEF
                     DPRKYIGAGRDAIQAVVESKIDDVLGSANSIN"
     gene            complement(3996..5627)
                     /locus_tag="PC414_002726"
     CDS             complement(3996..5627)
                     /locus_tag="PC414_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2465114.1"
                     /translation="MDNNLIGLIEHVENPAILCKESGEIIYCNHLIDSIFSFLDIKKP
                     RNINELDSNFDKTEILTDSKKKIAFRELRMTAHIYNMKDNNNENNIVYLFEKSLISDK
                     VIEDIIEHIDEVVVVFNKDGVIEKMNTVSDEILPFKRTEVLGRNITDLVRQGLVEEPI
                     ILNMLKVKKKIYRNIVYPDGKLIAYTAVPRWDSKGKLTGGVLTGRDISRVIKLESQIK
                     YSDISEDTEYISQSKIMDNIKKVVKRAAASDSSIFINGESGVGKEIIARTIYKYSSRR
                     DKPFIAINCGAIPNELLESEFFGYEEGSFTGAKKKGKKGLFEEANGGTIFLDEIGELP
                     MQMQKKLLRVIQENTITRIGGSKPIKIDVRYISATNISHEDLRNNLKFRQDLYYRLSV
                     IPVKIPPLRERKEDIVPLVNYFLKLYNEKYNREVEVSPKVIELLEEYSWPGNIRELKN
                     IIERFVVLSAKNVIGEDEFNMLINLDMIDNETDDLSPIVVNGIMNLNDAYKIVDQIMI
                     SKAINKYGSITKAAEVIGIYPSTIHRKIKSGHIHV"
     gene            complement(6110..7147)
                     /locus_tag="PC414_002727"
     CDS             complement(6110..7147)
                     /locus_tag="PC414_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDF2465115.1"
                     /translation="MNDIKDLSSYKNVWIFAEQREGKIAPVVIELLGEGRKLAKEVDA
                     ELCAILLGKDVDGLAKELITFGADKVYVADDALLEKYTTDAYTKVIKDAIDEIKPEIM
                     LFGATHIGRDLAPRIASRVGTGLTADCTKLEIDPEDKKIKQTRPAFGGNIMATIICPN
                     HRPQMSTVRPGVMDKAEKDETRTGEVIALDYKITQDDIRTTVLETVKTKKDLVSLTDA
                     NVIVSGGLGLGGPEGFEMLKKLADKLGGVVGSSRAAVDAGWIDHSHQVGQTGTTVKPN
                     LYIACGISGAIQHLAGMQSSDFIIAINKNPAAPILEIADYGVVGDLHEIVPMLIEKLD
                     SVDDLLEAIKA"
     gene            complement(7166..7951)
                     /locus_tag="PC414_002728"
     CDS             complement(7166..7951)
                     /locus_tag="PC414_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HDF2465116.1"
                     /translation="MKILVCVKQVPDTNEVRINKETGTLIRDGVPSILNPDDANALEE
                     ALKIKDEHDDVTITVITMGPPQADFMLRECLAMGADDAILLSDRAFGGADTWATSNTI
                     AAGISKVGDYDIIFAGRQAIDGDTAQVGPQIAEKLDIPQVTYVQDFKIEGKDIIVQRQ
                     LEDGYELIKVSTPVLLTAVKELNTPRYMSVDKIVKAYKHDVKVWTIDDLDVNKEEVGL
                     KASPTKVFRSFTPEPRGKGEILEGKADEIASKIIIGLKQKHII"
     gene            complement(7984..9117)
                     /locus_tag="PC414_002729"
     CDS             complement(7984..9117)
                     /locus_tag="PC414_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HDF2465117.1"
                     /translation="MLYNKEQELLRKAVRDFVSKELDTLPAEMDKTGVMPKELIKKLA
                     DAKFISSNIPEEYGGGGAGYVSYAIVMEEIARRCASTATFVTAGSSLASLPILYNGTE
                     EQKQKYLKGIATGELIGAFGLTEPGAGSDAGGQQTTAELVGDHYILNGRKTFITNGPF
                     CDVAIVIAVTDRSKGLRGTSAFIVESKWDGFSTGAHEDKMGIRGTETSDLIFENVKVP
                     KENLLGKEGQGFKIAMGTLEVGRIGVAALALGIAQGALDEAVKYTKQRVQFGKPIAKF
                     QNTQFTIADMETKVCAARGLVYDAAQKRDAGMRVAQESAMAKYYASEIANEVAYKALQ
                     LHGGYGFIKDYEIERMYRDARIVSIYEGTSEVQKMVISSNVLK"
     gene            complement(9165..10292)
                     /gene="hadC"
                     /locus_tag="PC414_002730"
     CDS             complement(9165..10292)
                     /gene="hadC"
                     /locus_tag="PC414_002730"
                     /EC_number="4.2.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     beta"
                     /protein_id="HDF2465118.1"
                     /translation="MEAILSKMKEVVENPNAAVKKYKSETGKKAIGCFPVYCPEEIIH
                     AAGMLPVGIWGGQTELDLAKQYFPAFACSIMQSCLEYGLKGAYDELSGVIIPGMCDTL
                     ICLGQNWKSAVPHIKYISLVHPQNRKLEAGVKYLISEYKGVKRELEEICGYEIEEAKI
                     HESIEVYNEHRKTMRDFVEVAYKHSNTIKPSIRSLVIKSGFFMRKEEHTELVKDLIAK
                     LNAMPEEVCSGKKVLLTGILADSKDILDILEDNNISVVADDLAQETRQFRTDVPAGDD
                     ALERLARQWSNIEGCSLAYDPKKKRGSLIVDEVKKKDIDGVIFCMMKFCDPEEYDYPL
                     VRKDIEDSGIPTLYVEIDQQTQNNEQARTRIQTFAEMMSLA"
     gene            complement(10292..11518)
                     /gene="hadB"
                     /locus_tag="PC414_002731"
     CDS             complement(10292..11518)
                     /gene="hadB"
                     /locus_tag="PC414_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     HadB"
                     /protein_id="HDF2465119.1"
                     /translation="MSEKKEARVVINDLLAEQYANAFKAKEEGRPVGWSTSVFPQELA
                     EVFDLNVLYPENQAAGVAAKKGSLELCEIAESKGYSIDLCAYARTNFGLLENGGCEAL
                     DMPAPDFLLCCNNICNQVIKWYENISRELDIPLIMIDTTFNNEDEVTQSRIDYIKAQF
                     EEAIKQLEIISGKKFDPKKFEEVMKISAENGRLWKYSMSLPADSSPSPMNGFDLFTYM
                     AVIVCARGKKETTEAFKLLIEELEDNMKTGKSSFRGEEKYRIMMEGIPCWPYIGYKMK
                     TLAKFGVNMTGSVYPHAWALQYEVNDLDGMAVAYSTMFNNVNLDRMTKYRVDSLVEGK
                     CDGAFYHMNRSCKLMSLIQYEMQRRAAEETGLPYAGFDGDQADPRAFTNAQFETRIQG
                     LVEVMEERKKLNRGEI"
     gene            complement(11533..12333)
                     /gene="hadI"
                     /locus_tag="PC414_002732"
     CDS             complement(11533..12333)
                     /gene="hadI"
                     /locus_tag="PC414_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyisocaproyl-CoA dehydratase activator
                     HadI"
                     /protein_id="HDF2465120.1"
                     /translation="MYTMGLDIGSTASKGVILKNGEDIVASETISSGTGTTGPSRVLE
                     KLYGKTGLAREDIKKVVVTGYGRMNYSDADKQISELSCHARGVNFIIPETRTIIDIGG
                     QDAKVLKLDNNGRLLNFLMNDKCAAGTGRFLDVMAKIIEVDVSELGSISMNSQNEVSI
                     SSTCTVFAESEVISHLSENAKIEDIVAGIHTSVAKRVSSLVKRIGVQRNVVMVGGVAR
                     NSGIVRAMAREINTEIIVPDIPQLTGALGAALYAFDEAKESQKEVKNI"
     gene            complement(12372..13571)
                     /gene="hadA"
                     /locus_tag="PC414_002733"
     CDS             complement(12372..13571)
                     /gene="hadA"
                     /locus_tag="PC414_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocaprenoyl-CoA:2-hydroxyisocaproate
                     CoA-transferase HadA"
                     /protein_id="HDF2465121.1"
                     /translation="MLLEGVKVVELSSFIAAPCCAKMLGDWGAEVIKIEPIEGDGIRV
                     MGGTFKSPASDDENPMFELENGNKKGVSINVKSKEGVEILHKLLSEADIFVTNVRVQA
                     LEKMGIAYDQIKDKYPGLIFSQILGYGEKGPLKDKPGFDYTAYFARGGVSQSVMEKGT
                     SPANTAAGFGDHYAGLALAAGSLAALHKKAQTGKGERVTVSLFHTAIYGMGTMITTAQ
                     YGNEMPLSRENPNSPLMTTYKCKDGRWIQLALIQYNKWLGKFCKVINREYILEDDRYN
                     NIDSMVNHVEDLVKIVGEAMLEKTLDEWSALLEEADLPFEKIQSCEDLLDDEQAWAND
                     FLFKKTYDSGNTGVLVNTPVMFRNEGIKEYTPAPKVGQHTVEVLKSLGYDEEKINNFK
                     DSKVVRY"
     gene            14248..15246
                     /locus_tag="PC414_002734"
     CDS             14248..15246
                     /locus_tag="PC414_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HDF2465122.1"
                     /translation="MKILVFGARDYEEPVIKKWSEEHKDVQVDIYPENMTEENVVKAK
                     GYDGISIQQTNYIDNPYIYETLKDAGVKVIASRTAGVDMIHFDLVNENGLIVTNVPSY
                     SPNAIAELAVTQAMNLLRKTPLVKKKVCEGDYRWIAELLGTEVRSITVGVIGTGKIGA
                     TSAKLFKGLGANVIAFDQYPNSDLNDILTYKDSLEDLLKEADLITLHTPLLEGTKHMI
                     NKDTLAIMKDGAYIVNTGRGGLINTGDLIEALESGKIRAAALDTFETEGLFLNKKMNP
                     GELTDPEINKLLSMEQVIFTHHLGFFTSTAIENIVYSSLSSAVEVIKTGTATNRVN"
     gene            15608..16135
                     /locus_tag="PC414_002735"
     CDS             15608..16135
                     /locus_tag="PC414_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04002 family protein"
                     /protein_id="HDF2465123.1"
                     /translation="MEKMNKVTSKKTTYIVTSALFASIICLTIAYILHIPVGGNNGYV
                     HIGDAFIYLAATILPTNYAIAASAIGAGLADLSTGAAIWVIPTIIIKPILVLFFTSKS
                     DKIINKRNIVASVVAGIVGLVLYMFAEGIIIGSFTSAFVMSLLGLLQPIGSFIVFIIL
                     GMALDKLDFKKRYFN"
     gene            16162..16758
                     /locus_tag="PC414_002736"
     CDS             16162..16758
                     /locus_tag="PC414_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04100 family radical SAM protein"
                     /protein_id="HDF2465124.1"
                     /translation="MNILYTIENSIYVNITNTCPCSCVFCIRNEKDEVANSGSLWLEH
                     EPSVDEVKEAFNKYNLDDYDEIVFCGYGEPLMRINELIEIAKFIKEKSSIKIRINTNG
                     LSDLIHNKKTAILLKNVIDAVSISLNAPNKEAYNRVTQPKFGEKSFDYMLDFARDCKK
                     YIKEVAFSVVDEISPEEIEESKQLAKKLDIPLRVRHKN"
     gene            complement(16823..18406)
                     /gene="asnB"
                     /locus_tag="PC414_002737"
     CDS             complement(16823..18406)
                     /gene="asnB"
                     /locus_tag="PC414_002737"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HDF2465125.1"
                     /translation="MINLEEKYLTRKDTYMIKILEYNGHIRNWKVLCEELGIDSSLCR
                     DERERAILVEAYKTWGYDMANHMHGMFAFALWDTVEKKLFCLRDQFGVKPFYYYETEN
                     GKLLYGSSIRKIMEQPGFVKELNEDMLQIYMSFTYVAGENTFFRGVKKLMPGHYLVYQ
                     NKTVCIGRYWKPEFHPDHSKSIEEWTDEIHTTLQKIMPEVKSENETAELFLSGGVDSS
                     YILAMSDIEKTGSCGYEEERFDESKLAQQTANILGCKNSRYIITPEEYFASVPYVMYH
                     MEQPTGDASAIVFAIGCKAAAENTNICYSGEGADEFFGGYNIYHKAECYGENLKTFYI
                     GNTNIMNEGEKRKILKKYNPDVLPIEVVKGIYEETEELDTLTNMSNVDIQIWLEGDIY
                     FNIDKMSTAVGLEVRMPLTDTRIFDIASRIPSEYKVKGKENKIAFRTAASKTLPEEIA
                     FRNKLGFIVPIRIWMADDRYNKDIRDKFNSEIADKFFNIDEINEIFNDYISGNSDNWR
                     KIWMIYTFLVWYEIYFVKC"
BASE COUNT         5807 a         3432 c         2292 g         7153 t
ORIGIN      
        1 tctatttcta taattaaaac tttttttatt tcgtattccc acactccata tatataacta
       61 cagacacatc aaaacacctt ttcaattctt tattcctaat tttattaccc tctaatctaa
      121 atattatatg tctatgaaac tgtctaaagc tcaagatttc tagcattatt ttcgacgtag
      181 caacactaca gtctcacaat gtggcgtatt tgggaacata tccatacact taaccatctt
      241 aatctcataa ccatacccct taaaatcaac caaatcaaca accaaagact taggattaca
      301 agaaatataa acaatctcct tagcaccaaa tccacaaata tccctaatag catccttatg
      361 aatgcctggt ctaggagggt caacaataat caaatcaggc ttgtccttta aatccttaac
      421 aaccttagcc acatccccag caataaactc acaattagta agtccattca acttagcatt
      481 ctcattagca gcaacaacag cttcctcaac tatctcaata ccatacactt tcttagcagc
      541 accagccata acctgtccaa tagacccagt accactatac aagtcaaata caactttatc
      601 attgtaatct ccaataaaat ccctagcaat actataaaga acctctgctc ccttagtatt
      661 ggtttggaaa aacgaaaatg gtgaaatctt aaacttaaga cctaaaattt cttcttgtat
      721 ataatctctt ccatacaata ctcttaactc atcacattgg actgcatctg ctaaaccatc
      781 attaatagta tgaagtacac ctactaactc agcttttaaa tcaatcccta aaagcatatc
      841 tttaaactca tacatatcaa aatcttcttg agaagaagtt actatattaa ccataatctc
      901 attagtatga atcccttttc taactacaag atgtctcaaa tatcctttgt gattcattgt
      961 tctataataa ggaagctttt tttcattgaa aaactctaca ctagaaacta atatctttga
     1021 gaagtcttca tcaaccaaca tacactcctc aacagtttgt atatctatat gctttccttt
     1081 tttatgaaga cctaatgtca aaggtccatt ttttacttca tctccaaaag tatattccat
     1141 cttatttcta taatatttgt tttctggact tccttctatc cctaaaaact gaaatccaaa
     1201 taaatcttgc tttaaaaata aatccataac ttgtttttct tttatttcta attgtttttc
     1261 atatggcaca gacaatagtg tacatcctcc acactctcta aagtgcttgc aagtttcttc
     1321 agtttcaagt ggtgagttct ctaataattc tatcatctta acttcagctt tcttacttct
     1381 aactttttta actccagctc tcaccttttg accacttatt ccacccttca tatggataac
     1441 tctatctcca acttggctta aagatgtacc accaaattcc attttatcta cttcaaattc
     1501 tataacatct tttttcttca cagacattcc tccaatttac tatagtatct ttattatctt
     1561 tcttcaatca attttacttc ttcatctgtt aactctctat actcacctaa ctctaaactt
     1621 tcatccaatt ttaaattgcc catagacaat ctttttaagt atactacttt tttgcctacg
     1681 ctttcaaaca ttctttttac ttggtgaaac ttaccttcat gtattgtaag ctctatttct
     1741 gattcatcat cactttttaa tatatttaac tgtgatggca tagttttata tccgtcatca
     1801 agcactacac cttctaaaaa agcttctaca tcttcttcag taactactcc atcaattttt
     1861 gcataataag tcttaggcac atgcttctta ggagataaaa ctctatgaga aagctttcca
     1921 tcattagtta aaactaataa tccctctgtg tctttatcta accttccaac aggaaatggt
     1981 tcaaaagcta agtatgataa atcaattaaa tctaatactg ttgggtcata tttatctgta
     2041 gttgctgata tatatccatc aggtttattc atcatcaaat atatatattc tctatatata
     2101 acttcttctc cattaaataa tatctcatca ttttcagtgt ctacttgagt tcctggattt
     2161 gaaacttcac ttccatttac tactacagac ccttgtttac aatacttctt tatttcactt
     2221 ctacttccat aacctaaatt tgaaagaatt ttatctatac gttgcttttt agccattaaa
     2281 aatgctctcc attctcataa tatttttctt tttcaaatac tgtaaatgct aaatctatac
     2341 tttctacatc taaattattt cttatactat cagttattat tcttgctact atatcttgta
     2401 cttcttgtcc tctatcaaac caagctactt caacaaaagg atatacatct gctattttac
     2461 catctcttat tgcaactgac tttatacatt ctatttcaaa gtagtctctt ggacatttta
     2521 cagcttcaac taaatcattt atcatttttt cacttatttt acatatatca ttttcattaa
     2581 ttcctcttat ttttatctgt ggcatagttt atctccttta ttttaaattt ttagtgaatt
     2641 atcatccact taattccttt cgataacgtt tgaagtggta acttcttggt caaggcaact
     2701 attgttatca gtatctccaa actaataagg tagttcctac ccttatagtt agcattatat
     2761 agctagtttt cctaaattgt ttatattctt tattatttta tttaatccta ttgtcttcta
     2821 tttaacatta tacttgtaat ttatagttat tacaatttac tatatacttt ttttgattta
     2881 acgttataat ataagtgatg ggtaaatttt aaaagttaat aggaggagtt taaaatggca
     2941 ttaattacta caaaagaaat gtttaaaaaa gcttacgaag gtggatttgc tataggtgca
     3001 tttaacataa gtgacttaga acaattacaa ggtgttttaa aagctgctaa agctaaaaat
     3061 tcttatgtta tgatacaagc ttctatgtca gctgttaaat atgctggacc tcatacatta
     3121 gttgaaatgg ttaaggctgc ttctgatgaa ataggtgtag atgtagctct tcacttagac
     3181 catggtccaa atatggatgc tattaaaact tgtattgatg ctggtttctc ttcagttatg
     3241 atagatggtt ctcattttga ctttgaagaa aatgttagaa taactaaaga agctgttgaa
     3301 tatgcacact ctaaaggtgt tgttgttgaa gcagaacttg gtgttctagc tggaacagaa
     3361 gatgatgtta cttctgatgt tcacaagtat actcaaccag ctgaggcagt tgagttcgtt
     3421 gaaagaacag gtgttgattc attagcaata gctattggaa cttctcatgg tgctttcaaa
     3481 ttcaaaggag aagctaaatt aagattcgat atactagaag aaatccaaag caaattacca
     3541 ggtttcccaa tagttcttca tggtgcatct gctgttgacc aaaacgctgt tgctacatgt
     3601 aatgaatttg gtggaaatat agctggtgct aaaggtgtac ctgtagatat gttaagaaaa
     3661 gcatcttcta tggctgtttg taaaataaac atggatactg acttaagact agctatgact
     3721 gctgctatca gaaaattctt agctgaaaat ccaaaagaat ttgacccaag aaaatacatt
     3781 ggtgctggta gagatgctat acaagctgta gttgaaagca agatagatga tgttttaggt
     3841 tctgctaact ctataaacta atataacttt taaatattta taaaagaaaa gagatgactt
     3901 aaagtaatta aatttatttt taagccatct cttttaagtt gtataaatta tgtatcttat
     3961 aatattttaa ttatattgtc tttttaaact gacagctaaa cgtgaatatg tccactcttt
     4021 atctttctat ggatggtaga aggatatata cctataactt cagcagcctt tgttattgaa
     4081 ccatacttat ttatagcctt acttatcatt atttggtcca ctattttata tgcatcatta
     4141 agattcataa ttccatttac cactattggt gataaatcat ctgtttcatt atcaatcata
     4201 tctaaattta tcagcatgtt aaattcatct tctccaatta catttttagc tgataatact
     4261 acaaatcttt caatgatgtt ttttaattct cttatgtttc ctggccaaga atattcttct
     4321 aataactcaa taactttagg cgaaacttct acttctctat tgtacttttc attgtaaagt
     4381 tttaagaaat aatttactaa tggtactata tcctcttttc tttctctaag aggaggtatt
     4441 tttactggaa taacacttaa tctatagtac aaatcttgtc taaatttaag gttatttcta
     4501 aggtcttcat gagaaatatt agtagcactt atatatctaa catctatttt tataggctta
     4561 ctaccaccta ttctagtaat agtattttct tgtataaccc taagtaattt tttttgcatc
     4621 tgcattggta actcacctat ctcatccaag aaaatagttc caccattcgc ctcttcaaaa
     4681 agtccctttt tacctttttt cttagctcca gtaaaagaac cttcttcata tccaaaaaac
     4741 tctgactcta ataattcatt tggtatagct ccacagttta tagctataaa aggtttgtct
     4801 ctccttgaac tatacttata tattgttctt gctattattt cttttcctac acctgattct
     4861 ccatttataa atatagaaga atctgaggct gcagctctct taacaacttt ttttatattg
     4921 tccataatct tactttgact tatatactct gtgtcttctg atatatcact atattttatc
     4981 tgagattcaa gcttaataac ccttgaaata tctctccctg ttagtactcc tcctgtaagc
     5041 tttcccttag aatcccatct tggaactgcc gtatatgcaa taagcttccc atctgggtaa
     5101 acgatatttc tataaatctt tttcttcact ttaagcatgt ttaatattat tggttcttca
     5161 actaatcctt gtctcacaag gtctgttata tttctaccaa gtacctcagt tcttttaaaa
     5221 ggtaatatct catcactgac agtgttcatc ttttcaatta ccccatcttt gttaaacaca
     5281 acaacaactt catctatatg ctctattata tcttcaatta ctttatcaga tattagagac
     5341 ttctcaaata aataaactat attattttca ttattattat ctttcatatt gtatatatgt
     5401 gcagtcatcc ttaactctct gaaagctatt ttctttttac tatctgtcag tatttctgtt
     5461 ttgtcaaaat ttgaatctaa ttcatttata tttctaggct tttttatatc tagaaaacta
     5521 aaaatactat ctattagatg attacagtat attatttcgc ctgattcttt acataaaatg
     5581 gctggatttt ctacatgttc tataagccct attaaattat tatccattaa tattcgcacc
     5641 tttcattaag taatttattg aactacatac aaaatttatt gtttgcttac aataaatacc
     5701 atcagcacat ataatcaact ccttataata ttcgtctttt caagtagttt atttcattaa
     5761 aacgtaacta agatataatt ttaatgatta tagtttgtta aaaatttcat tttgctttta
     5821 attgtatttt atcacatatt tagtaattat ttgtctattt aatacaattt aattaaattt
     5881 aagatttatt taaaaaaaca aagataatcc ttacagaata taactcacaa tttgtaagca
     5941 atatacatat aaggattatc cagattatct tgtattataa aattttattc aaacagactt
     6001 tttaataact gacaaataca cactagcaat aacttatcaa agggttaata attatgttac
     6061 taactaataa gacttattca atcagtaata tttatatagt ttgtatctat taagctttta
     6121 tagcttctaa taaatcatca acgctatcta atttttctat aagcattgga actatttcat
     6181 gtaagtctcc aactactcca tagtcagcga tttctaaaat tggagctgct gggtttttgt
     6241 ttatagcaat tatgaaatct gatgattgca tacctgctaa atgttgtatt gctcctgata
     6301 taccacaagc tatatatagg tttggtttaa cagtagttcc tgtttgacct acttggtgag
     6361 aatggtctat ccatccagca tcaacagccg cacgagaaga accaactact ccacctaatt
     6421 tgtcagctaa tttcttaagc atttcaaatc cttctggtcc acctaatcct agaccacctg
     6481 atactataac atttgcatct gtaagagata ctaaatcttt cttagtttta actgtttcta
     6541 aaacagtagt tcttatatca tcttgagtta ttttgtagtc taatgctata acttcaccag
     6601 ttcttgtttc gtctttttca gctttatcca taacaccagg tctaactgta gacatttgag
     6661 gtctatggtt tggacaaatg attgtagcca ttatgtttcc accaaatgct ggacgagttt
     6721 gttttatttt cttatcttct gggtctattt ctaacttagt acagtcagct gttaatccag
     6781 ttccaactct tgaagctatt ctaggtgcta agtctctacc tatatgagtt gctccgaaaa
     6841 gcattatttc tggttttatt tcatctattg catcttttat tacttttgta tatgcatcag
     6901 ttgtatattt ttctaaaaga gcatcatctg caacataaac tttgtcagct ccaaaagtga
     6961 ttaattcttt agctaatcca tcaacatctt ttcctaataa tattgcacaa agttctgcat
     7021 ctacttcttt agctaatttt cttccttctc ctaataattc tataactact ggagctattt
     7081 ttccttctct ttgttctgca aatatccata cgtttttata agaacttaaa tcttttatat
     7141 cattcatagt ttgtactcct ccaacttata taatgtgctt ttgttttaga ccgattatta
     7201 ttttactagc tatttcatca gctttacctt ctaagatttc tccttttcct cttggttcag
     7261 gagtaaatga tctaaatact ttagttggtg atgcttttaa accaacttct tctttattta
     7321 catctaaatc atcaattgtc caaactttta catcatgttt atatgctttt actattttat
     7381 caacagacat gtatctaggt gtatttaact cttttactgc tgttaaaagt acaggtgtac
     7441 ttacttttat taattcgtat ccatcttcta attgtctttg aactattata tcttttcctt
     7501 ctattttgaa atcttgtaca tatgtaactt gaggaatgtc taatttctct gcaatttgtg
     7561 gtccaacttg tgcagtatct ccatctattg cttgtcttcc tgcaaatatt atgtcatagt
     7621 cacctacttt agatattccc gctgcaatag tatttgatgt agcccaagta tcagctcctc
     7681 caaatgctct atcacttaat aatattgcat catcagcacc catagctaaa cactctctta
     7741 gcataaagtc tgcttgagga ggtcccattg ttataactgt tatagttaca tcatcatgct
     7801 catctttaat ttttaaagct tcctctaatg catttgcatc atctgggttt aatatacttg
     7861 gaactccatc tcttataaga gtacctgttt ctttatttat tcttacttcg ttagtatctg
     7921 gaacttgttt tacgcaaact aatattttca aagtctatac ctccaatttc attagattat
     7981 ttattatttt aatacgtttg atgaaattac cattttttga acttctgatg ttccttcgta
     8041 tattgatacg attctagcat ctctgtacat tctttcgatt tcataatctt ttataaatcc
     8101 atatccaccg tgaagttgta atgctttata agcaacttca tttgcaattt ctgatgcata
     8161 gtatttagcc atagcagatt cttgagcaac tctcattcct gcatctctct tttgtgctgc
     8221 atcataaact aatcctctag ctgcacaaac ttttgtttcc atgtcagcta tagtaaattg
     8281 agtattttgg aatttagcta taggcttacc aaattgaact ctttgttttg tatatttaac
     8341 agcttcatct aaagcacctt gagctattcc tagagctaag gcagcaacac ctattctacc
     8401 aacttctaga gtacccattg ctatcttaaa tccttgacct tcttttccaa gtaagttttc
     8461 ttttggaact ttaacgtttt caaatattaa gtcagaagtt tcagttcctc ttatacccat
     8521 cttatcttca tgagctcctg ttgagaaacc atcccattta ctttctacta taaacgctga
     8581 tgttcctcta agtcctttac ttctatctgt aactgctatt actatagcta catcacagaa
     8641 tggtccatta gttatgaaag tttttctacc atttaatata tagtggtctc caactaattc
     8701 tgctgttgtt tgttgtccac ctgcatcaga accagctcct ggctcagtta aaccaaatgc
     8761 tcctattaat tcacctgttg ctataccttt taagtatttt tgtttttgct cttcagtacc
     8821 attgtaaagt attggtaatg aagcaagaga agaaccagct gtaacaaaag tagctgttga
     8881 agcacatctt ctagctattt cttccattac tatagcgtaa gaaacatatc ctgctccccc
     8941 accaccatat tcttctggga tattactgct tataaatttt gcatcagcta attttttgat
     9001 taattcctta ggcattacac ctgttttatc catttctgct ggtaaagtat ctaattcttt
     9061 actaacaaaa tctcttactg cttttcttaa aagttcttgt tctttgttat ataacatagc
     9121 ttcctccaat tttcatattt tatagtaaat ttattttaat gaacttacgc taaactcatc
     9181 atctcagcaa aagtttgaat acgagttctg gcttgttcat tattctgagt ttgttggtcg
     9241 atttcaacat ataaagtagg tattccacta tcttctatat cttttctaac taaaggataa
     9301 tcgtattctt ctgggtcaca gaatttcatc atacagaaga taacaccatc tatatctttc
     9361 tttttaactt catctactat aagtgaccca cgttttttct tagggtcata agctaatgaa
     9421 catccttcta tgtttgacca ttgtcttgct aatctttcta acgcatcatc acctgctggt
     9481 acatctgttc tgaattgtct tgtttcttgt gctaagtcgt cagctacaac tgatatattg
     9541 ttgtcttcta aaatgtctaa tatatcttta gaatcagcta atatacctgt taataaaact
     9601 ttctttccag aacagacttc ttctggcata gcatttaatt ttgctattaa atctttcact
     9661 agctcagtat gttcttcttt tctcataaag aacccactct taattactaa gcttcttatt
     9721 gatggtttta tagtattaga atgtttataa gctacttcaa caaagtctct catagttttt
     9781 ctatgttcat tgtaaacttc tatactttca tgaatttttg cttcttctat ttcatatcca
     9841 caaatttctt caagttctct ttttacgcct ttgtactcac tgattaagta ttttacacca
     9901 gcttcaagtt ttctattttg tgggtgtact aatgatatat attttatatg aggtactgct
     9961 gatttccagt tttgtcctaa acaaattagt gtatcacaca tacctggtat aataactcca
    10021 gataattcat cataagcacc ttttaatcca tattctaaac atgattgcat tattgaacat
    10081 gcaaatgcag ggaaatattg tttagctaaa tctaattctg tttgccctcc ccatatacca
    10141 actggaagca ttccagctgc atgtataatt tcttctgggc aataaactgg gaaacaacct
    10201 atagcttttt taccagtttc acttttatat ttttttacag ccgcatttgg attttcaact
    10261 acttctttca ttttagataa aatagcttcc attatatctc acctctatta agtttttttc
    10321 tttcttccat tacttcaact aaaccttgaa ttcttgtttc aaattgagca ttagtgaaag
    10381 ctctagggtc tgcttggtca ccatcaaatc cagcatatgg taatccagtt tcttcagctg
    10441 ctcttctttg catttcatat tgtattaaac tcataagttt acagcttctg ttcatatgat
    10501 agaatgctcc atcacattta ccctctacta aagaatcaac tctatatttt gtcatacggt
    10561 ctaggtttac attgttaaac atagtactat atgctactgc cattccatct aaatcattaa
    10621 cttcatattg taatgcccaa gcatgtgggt aaacactacc tgtcatgtta actccaaatt
    10681 tagctaatgt tttcatcttg tatcctatat atggccaaca aggtatacct tccatcatta
    10741 ttctgtattt ttcttcccct ctgaaagaag atttaccagt tttcatgttg tcctctaatt
    10801 cttctataag taacttaaat gcttctgtag tttctttttt acctctagca caaactatta
    10861 cagccatgta agtaaataag tcaaatccat tcattggaga aggagaagaa tctgctggta
    10921 aactcataga atacttccat agtcttccgt tttcagctga tattttcatt acttcttcaa
    10981 acttcttagg gtcaaatttc tttcctgata taatttctag ttgttttata gcttcttcaa
    11041 attgagcttt aatataatct attcttgatt gagtaacttc gtcttcatta ttgaaagttg
    11101 tatcaatcat tattaaaggt atatctaatt ctcttgaaat attttcatac cattttataa
    11161 cttggttaca tatattgtta cagcaaagta ggaaatctgg agctggcata tccaaagctt
    11221 cacatccacc attttctaaa agaccaaaat ttgttcttgc atatgcacat aggtcaatag
    11281 aatatccttt agattcagct atttcacata attctaaaga acctttttta gctgctactc
    11341 cagctgcttg gttttctgga tataatacgt ttaagtcaaa tacttctgct aactcttgag
    11401 gaaatactga tgttgaccaa cctacaggtc ttccttcttc tttagcttta aatgcatttg
    11461 catattgttc agctaataaa tcattaatta ctactctagc ttcttttttt tcagacattt
    11521 tcatccctct ttttatatat ttttcacttc tttttgtgat tcttttgctt catcaaaagc
    11581 atataacgct gctcctaaag caccagttaa ttgaggtata tcaggtacaa taatttctgt
    11641 gttgatttct cttgccatag ctcttacaat accactattt ctagcaactc caccaaccat
    11701 aactacattt ctttgtactc ctattctttt tactaggcta gaaactctct ttgctactga
    11761 agtatgaata cctgccacta tatcttcaat ttttgcattt tcagataaat gtgatataac
    11821 ctcagactct gcaaatactg tacatgtact gcttattgat acttcatttt gagaattcat
    11881 agatatactt ccgagttcag atacatcaac ctctattatt tttgccatta catctaaaaa
    11941 tcttcctgta cctgcagcac atttgtcatt cataagaaag tttaatagtc ttccattatt
    12001 atctaatttt aatacctttg catcttgacc acctatgtca ataatggttc ttgtctctgg
    12061 aattatgaaa tttacccctc tagcatgaca gcttaattca cttatttgct tatcagcatc
    12121 tgaatagttc attcttccat atcctgtaac tacaactttt ttaatatctt ctcttgcaag
    12181 acctgtcttg ccatataatt tttctaaaac tcttgatggt ccagtagtcc cagtaccaga
    12241 ggatattgtt tcagaagcta caatatcttc cccattcttt aagattactc cctttgatgc
    12301 agttgaacct atatctaatc ccattgtgta catctttagc attctccttt taaaaaatct
    12361 agaattacta attaatatct tacaacttta ctatctttaa agttatttat tttctcttca
    12421 tcgtagccta aagattttaa tacttctaca gtatgttgac ctacttttgg tgctggtgta
    12481 tattctttaa ttccttcatt tctaaacata actggagtat taactaagac acctgtattt
    12541 ccgctatcgt atgttttctt aaataagaag tcatttgccc aagcttgttc gtcatctaat
    12601 aaatcttcac agctttgaat tttttcaaat ggtaagtctg cttcttctaa taaagctgac
    12661 cactcgtcta atgttttttc taacatagct tctccaacta tcttaactaa atcttcaaca
    12721 tgattaacca ttgaatctat gttattatat ctatcatctt ctaatatata ttctctattt
    12781 ataaccttac agaatttgcc taaccacttg ttgtattgta ttaaagctaa ttgaatccat
    12841 cttccatctt tacatttata tgtagtcatt aatgggctgt ttggattttc tcttgataaa
    12901 ggcatttcat ttccgtattg tgctgttgtt atcattgttc ccattccata tatagctgta
    12961 tggaaaagac ttactgttac tctctcacct ttaccagttt gagctttttt atgtaatgct
    13021 gctaaacttc ctgctgctag tgctagacct gcatagtggt caccaaatcc tgctgctgta
    13081 tttgctggag atgttccttt ttccataaca gattggctaa ctcctcctct tgcgaagtat
    13141 gcagtatagt caaatcctgg tttatctttt aaaggtcctt tttcaccata tcctaatatt
    13201 tgagagaata ttaatcctgg atacttatct tttatttggt cataagctat acccattttt
    13261 tctaatgctt gaactctaac attagttaca aatatgtctg cttctgataa taatttatgt
    13321 aatatttcta ctccttcttt tgattttaca ttaatactta cacccttttt atttccattt
    13381 tctaattcaa acatagggtt ttcatcatct gatgctggag atttaaatgt tccacccata
    13441 actcttattc catcaccttc tataggttca atcttaataa cctctgcacc ccagtcacct
    13501 aacatttttg cacaacatgg tgctgcgatg aaacttgaaa gttctactac tttaactcct
    13561 tctaaaagca taacgttttc ctccttaata gtatcaatta taaaatttcg attaaatcac
    13621 tcataaaata cttttttctg atattaactg tctgatgtca aagttcctaa tcttatctaa
    13681 aataacttaa aaattattaa aattattttt tattgtttta ttgtcttaca cctatattgt
    13741 taagcattta tcgtgccaac tttatattta atcaaaatat cgcttgattt gaatgttttt
    13801 taacaatatg caattgaata atctttttca tatcgtggca tcttgccact atccattgca
    13861 ttttgctatt ctttctatgc ttataaaagt tttttttata tattttttta tgaaaaagct
    13921 tgacatttca aaaaaagttt tcattgctaa actcaaaaac acatgttatc attgtaatta
    13981 atcagcaagt tagttaacta atttcaaact tagataattt tttaataata tttaatctaa
    14041 aaaataacaa tttctttgat aaattttaga gtaattatta cattaaataa aaaattacta
    14101 aatatcaaaa taacacaggg aaaacaattt caaaactaaa tttttaacct taaaaatttt
    14161 gtataaaaaa tataacctta tatgctacat attatttcta aatgaaaaat taccataata
    14221 atttaataat tacaggaggg tattgatatg aaaatactag tatttggagc acgcgattat
    14281 gaagaaccag taataaaaaa atggtctgaa gaacataagg atgttcaagt ggatatttat
    14341 cctgaaaaca tgactgaaga aaatgtagtt aaagctaaag ggtatgatgg tatatctata
    14401 caacaaacta actatataga taatccttat atttatgaaa ctttaaaaga tgctggggtt
    14461 aaagttatag cttcaagaac tgcaggggtt gacatgatac attttgattt agttaatgaa
    14521 aatggactta tcgttacaaa cgttccttct tattcaccta atgcaatagc tgaattagct
    14581 gttactcaag ctatgaacct tttaagaaag actcctctag taaagaaaaa agtctgtgaa
    14641 ggtgattacc gttggatagc tgaacttctt ggaacagaag ttagatctat tacagttggt
    14701 gttataggta caggaaaaat aggtgctact tctgcaaaat tattcaaagg cctaggagct
    14761 aatgtaattg catttgacca atatccaaat agtgatttaa atgatatatt aacttacaaa
    14821 gattctttag aagaccttct aaaagaagct gaccttataa cattacatac tcctttactt
    14881 gaaggaacaa aacatatgat aaataaagat actctagcta taatgaagga tggagcttac
    14941 atagtaaata ctggccgtgg tggtttaatt aatacagggg atttaataga agcactagag
    15001 tcaggaaaaa ttagagctgc tgcccttgat acatttgaaa ctgaaggatt gttcttaaac
    15061 aaaaaaatga atcctggaga attaactgac ccagaaataa ataaacttct ttctatggaa
    15121 caagttatat tcactcatca ccttggtttc ttcactagta cagcgattga aaatatagtt
    15181 tattctagtt taagtagtgc tgtagaagtt ataaaaacag gaactgctac taatagagta
    15241 aattagtatt ttataaatat attttttcat taattagcta ttatctaaca aaatagcctt
    15301 taatttccca aatataagag agtcagctca gtatatattg agtagactct cttatattat
    15361 tttttatatt aaacttgtac tcttcttatt cataccaaat taaatttata ttaacttatt
    15421 ctaactactc ctactctata cattttacct atttttactt ttctaattat atacatttga
    15481 atgggtacaa tctgtatttt acaaaaaaaa cacctaacaa actatgaaaa ttgtatgatt
    15541 aatattaata tatttataag aaaacataac aattatgtta gctctggatt tttagggagg
    15601 gagtatattg gaaaaaatga ataaagttac ttctaaaaaa actacctata ttgttacttc
    15661 agcattattt gcatccataa tatgcctgac aatagcatat atattacata ttccagttgg
    15721 tggaaacaat ggatatgttc atataggaga tgcttttatt tatctagctg caactatttt
    15781 gccaacaaac tatgctatag ctgcttctgc aattggtgct ggtctagctg atttatctac
    15841 aggtgctgct atctgggtaa taccaactat aatcataaaa ccaatacttg tattattttt
    15901 cacttctaaa agtgacaaga taataaataa gagaaatata gttgcttcgg ttgtagcagg
    15961 tatagtagga ttagttttat acatgtttgc tgaaggtata attattggaa gttttacaag
    16021 tgcatttgta atgagcttac ttggcttatt acagccaata ggaagtttta ttgtttttat
    16081 aatcttaggt atggcacttg ataaacttga ttttaaaaaa cgttatttta attaagaatt
    16141 taaatttatg gagggaaaat tatgaatatc ttatatacaa ttgaaaatag tatctatgta
    16201 aatataacta atacatgtcc atgtagttgt gttttttgta taagaaacga aaaagatgaa
    16261 gttgcaaata gtggtagttt atggttggag catgagccaa gtgtagatga agtaaaagaa
    16321 gccttcaata aatacaattt agacgactat gatgaaatcg tattttgtgg atatggcgaa
    16381 ccattaatga gaataaatga attgatagag atagcaaagt ttataaaaga aaaaagtagc
    16441 ataaagataa gaataaatac aaatggtcta agtgatttaa tacataacaa aaaaacagct
    16501 attctgttaa aaaatgttat agatgcagta tcaataagtc ttaatgcccc aaataaagaa
    16561 gcttataata gagttactca accaaagttt ggagaaaaat catttgatta tatgttagat
    16621 tttgcaagag attgcaaaaa atatataaaa gaagtagctt tttcagtagt agatgaaata
    16681 tctccagaag aaatagaaga atctaaacaa ctggcaaaaa aacttgatat accattaaga
    16741 gttagacata agaattagca agtaaaatta taaaacatta agaatcacct gaatggtatt
    16801 tcaggtgatt cttcctcttg tattaacatt ttacaaaata gatttcatac cacactagga
    16861 atgtgtaaat catccaaatc tttctccaat tatcagagtt tccactaatg tagtcattaa
    16921 aaatctcatt aatctcatct atgttaaaga atttatctgc tatctcacta ttgaatttat
    16981 ctcggatatc cttattatat cggtcatcag ccatccatat acgaattggc acaataaatc
    17041 ctaatttatt tctaaaagca atctcttctg gtaatgtttt tgatgcagct gtacgaaagg
    17101 caattttatt ctcttttcct ttcactttat attctgatgg tattctggat gcaatatcaa
    17161 atattcttgt atctgtaaga ggcattctga cttccaatcc tactgcagta ctcattttat
    17221 ctatattaaa ataaatatct ccttccaacc atatttgaat atctacattt gacatgttag
    17281 taagtgtatc tagttcttct gtttcttcat aaattccttt taccacttca ataggaagca
    17341 catcaggatt gtatttctta agaatttttc ttttctcacc ctcattcata atgtttgtat
    17401 ttccaatata aaatgtcttc aaattctctc cataacactc tgccttatgg tatatattat
    17461 atccaccaaa aaactcatct gctccttctc cagagtaaca gatatttgtg ttttcagctg
    17521 ctgccttgca tccaatagca aaaacgatag cagatgcatc tccagttggt tgctccatat
    17581 ggtacattac ataaggtact gacgcaaaat actcttcagg tgtaattata tatctcgaat
    17641 ttttacaccc aagaatatta gctgtctgtt gtgctaattt tgattcatca aaacgctctt
    17701 cctcataacc acatgaacct gttttttcta tatctgacat tgccaatata taagatgaat
    17761 caacacctcc tgataggaat aactctgcag tttcattctc tgattttacc tctggcataa
    17821 ttttctgaag tgttgtatga atttcatccg tccattcctc aatagacttg ctgtggtcag
    17881 gatgaaattc aggtttccaa taacgaccaa tacatactgt tttattctga tagacaaggt
    17941 aatgtcctgg cataagcttc tttactccac ggaagaatgt attttcacct gctacatatg
    18001 tgaaagacat ataaatctgt aacatgtctt catttaactc tttcacaaat cctggttgct
    18061 ccataatctt acgaatagat gaaccataga gaagcttccc attttcagtc tcataataat
    18121 agaaaggttt taccccaaac tggtctctta ggcagaagag tttcttttcc acagtatccc
    18181 ataatgcaaa agcaaacatt ccatgcatgt gatttgccat atcatatccc catgtcttat
    18241 atgcttccac taaaattgct cgttcacgct catctctaca taatgaagaa tcgattccaa
    18301 gttcttcaca aagaactttc cagtttcgaa tatgcccatt gtattctaga atttttatca
    18361 tatatgtgtc ttttcgtgtt aaatactttt cttctaaatt tatcatatgt gtatcctttc
    18421 gtattaaaac atttaacttc taaatttatt aaatgtttat atatatttat tcacttataa
    18481 tcactgaaaa atcgactttt ttcaaagtaa attctttttg aattagtact aatctgctct
    18541 ttattaatgg cataaatatg attatcaagt attatcagtg ttataacatt gaatttttta
    18601 ttcttcataa atattgcccc ctttatatta ccaactaaat ataatatatc ttatgttact
    18661 tacatattct ttaagaaatt ctga
//
LOCUS       DAMJVK010000078        18576 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.079, whole genome shotgun sequence.
ACCESSION   DAMJVK010000078 DAMJVK010000000
VERSION     DAMJVK010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18576
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.079"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            835..2505
                     /locus_tag="PC414_002738"
     CDS             835..2505
                     /locus_tag="PC414_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF and EAL domain-containing protein"
                     /protein_id="HDF2465126.1"
                     /translation="MREESNSLSLDHIEIFFELLSQSTEDYIFFWDINRNKFKISSAI
                     FDEFNLSKEIESDLVNCWSKIVYPDDVQIWKDDIQELLHGKKGEHNLEYRLINKYKEI
                     VWISCRGKVYVSDDPKTIFLVGRIKNIGEKNKFDSITGTWNREQFEHRMNYLIKEKIY
                     KNGAMFIMDIDNFKNINEKYGHSYGDKVLRAIATEVLEYLPKDVRLYRLDGDEFAFFY
                     PMCTKETIEKIYEKIQMYTNTQHEIESNKYYCTVTAGVAMYPEDGDNYLDLFKHADIA
                     LDIAKISGKNRIKFFSQELYENKLKVISMQQKLRECVENNFNDFELFFQPQVNAVTKE
                     VIGAEVLLRWHSSTYGEVSPVEFIPILEQSNLIIPVGKWIIKEAVKQCKEWHKINPDF
                     KISVNVSYIQLKEDFFRDFIVECLVEYQLRPEFLILELTENCWIPDINLLNDKFISLK
                     GIGVYIAIDDFGTGYSSLNYLKELSVNIIKIERSFVKNITYNSYEYTFLEYIIKLAHI
                     INLKVCVEGIESYEEYDIVKSLGVDIIQGFLFGRPVSASEFYKFQLSK"
     gene            complement(2627..3361)
                     /gene="pflA"
                     /locus_tag="PC414_002739"
     CDS             complement(2627..3361)
                     /gene="pflA"
                     /locus_tag="PC414_002739"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HDF2465127.1"
                     /translation="MIKGKIHSIETFGTVDGPGIRYILFFQGCPLRCKYCHNRDTWDI
                     KSGKEYTVDEIITDALKYTSFMKFSGGGITASGGESTLQPEFLSELFKKAKENDIHTC
                     LDTSGFVDIETIDPVLDNIDLVLLDLKHMVEEKSIDLTGVGMDKALKLARHLESRNIP
                     VWIRHVLVPGITDDIDNLEKLGQFVATLKNVERFELLPYHSMGIHKWESLGIDYELKD
                     VPDATKEDVQKASEIISKFGVKVYNS"
     gene            complement(3521..5752)
                     /gene="pflB"
                     /locus_tag="PC414_002740"
     CDS             complement(3521..5752)
                     /gene="pflB"
                     /locus_tag="PC414_002740"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDF2465128.1"
                     /translation="MNAWQGFKTGRWTKEINVREFIQLNYSPYEGNDSFLAGATENTK
                     KLWDEAMVLFKKERENGGTLDVDTKTVSGIAAYAPGYLDKELETIVGLQTDAPLKRAV
                     MPYGGIKMVENSCEAFGYELDPEIKDIFTKYRKTHNQGVFDVYTPEMRAARKSGIITG
                     LPDAYGRGRIIGDYRRVALYGVDALIEDKNEQKKSLEVSCMDEEVIRLREEITEQISA
                     LNELKKMAESYGFDISKPATNSKEAVQWLYFGYLGAVKDQNGAAMSLGRTSTFLDIYF
                     ERDLKAGIVTEEELQEYMDHFVMKLRMVKFLRTPDYNNLFSGDPTWVTECIGGMGIDG
                     RTLVTKNSFRMLNTLYTLGPSPEPNLTVLWSTKLPQGFKDFCSKVSIDTSSVQYENDD
                     LMRAYWGDDYGIACCVSAMRIGKQMQFFGARVNLAKTLLYAINGGVDEKSGVQVGPRF
                     EPITSEYLDYDEVMSKFEPFTDWLATLYVNTLNVIHYMHDKYSYEALEMALHDRDIFR
                     TMACGMAGLSVCADSLSAIKHAKVKTIRNEQGIAVDFEIEGDYPKYGNNDDRVDSIAV
                     ELVESFMNKIRKNKTYRNSYPTQSILTITSNVVYGKKTGNTPDGRRAGAPFAPGANPM
                     HGRDTNGALASLSSVAKLPYEHAQDGISNTFSIVPGALGKDMTERINNLSAMMDGYFA
                     QNAHHLNVNVFDRATLEDAMEHPEEYPQLTIRVSGYAVNFIKLTKEQQLDVINRTFHG
                     KMA"
     gene            6045..7106
                     /locus_tag="PC414_002741"
     CDS             6045..7106
                     /locus_tag="PC414_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HDF2465129.1"
                     /translation="MEKRKSIHLHKLNIYLIGGGDRLFITVVLFLVGFLLITKGADIF
                     INCTVEIGKKTNISEIILGATIVSFATTLPEFTVSLLASIDGHTTMSLGNAVGSIICN
                     TGLALGLVVFISPFNVDKKMFFSKSLLLIVSVIVLILLSLDGVITRGDSLLLIIILIF
                     YMVNNYRSVVGKSDTKNRNIKNNTIKDISTKKGKNYRGFSVLEIVKILLLFATGLIMM
                     IIGSQILIESGVIIASFLNIPQGIVSLTIIALGTSLPEIVSSITAIRKNHHEISVGNI
                     LGANILNIVSVIAVSAIPNNIPILSQNRQLDIPFMILLLLIVIIPTLKSNKLSRIQGI
                     LMLFTYFLYISILYFMYII"
     gene            7197..8810
                     /locus_tag="PC414_002742"
     CDS             7197..8810
                     /locus_tag="PC414_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF2465130.1"
                     /translation="MNITKFEDLPISEGIKKAIAEMGFEEPSPIQAQSIPAILSGKDV
                     IGQAQTGTGKTAAFSIPILETIDPNNRSLQAVVLCPTRELAIQVSTEIRKLAKYSHGI
                     KTLPIYGGQPIDRQIKSLKSGVQVVIGTPGRTIDHINRKTLKMDNVKMIILDEADEML
                     DMGFREDIEMILSKIPEERQTTFFSATMPRGILELTKRYQKDPEHIKVVRKELTVSNT
                     KQYYIETRSSNKLEVLCRLVDVYDPKLSVVFCNTKRKADELVGDLQARGYFADALHGD
                     LKQTQRDIVMDKFRNGTIDILVATDVAARGIDVDDVECVFNYDLPQDEEYYVHRIGRT
                     GRAGREGMSFTFVFGKEMRKMKDIERYTKSKLIKHNIPTITDVEEKKVGTFFAQVKQT
                     IEEGHLTKQLQWLEGFCNDEDYAMVDIAAALVKLSLGEEMKEEIIEEKPRRERGDRKG
                     GTGAKDGMIRLFINIGRNQRVQAKDIVGAIAGEVGIPGKVVGTIDIYDKYTFVEIPKK
                     DAKTVIEKMKDIKIKGNKINIEKANKKKK"
     gene            9085..11070
                     /locus_tag="PC414_002743"
     CDS             9085..11070
                     /locus_tag="PC414_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDF2465131.1"
                     /translation="MSMTYMDPRVFLILEMVLEEDTNTLEQFSNVLGVSTRTIRNYIN
                     QINRELKGGIAKIVKNEKGTYSIRIEDESKLIEIVNFNRGKLSNFINLNSPDERINYV
                     LDILTMTNNPITIDDLAEEICIGRTTLIKDLKKADRILAEYDLELKRKPNIGMILVGE
                     EMDIRLLILDRLYENYIDVLENVNQINLVKDYDIECLRDELKKLFKKEELYITEQVLR
                     DVERYIIMSVLRNLNGYKFEKIDKRFEVIRCSDEYTLGLKLKALLEKIFNIVLNEKET
                     IFLTMPLIGRKAPSTKLALSSIKINDSVKEVVDEVTSFLLETSGIDFKEDKKLIENLE
                     YHLYFALNRMRFNIRVKNPLLQEIKERYTFPYSVAKIAAMIIEEKFDLKICDHEIGYI
                     ALHFGSYFEKNSRKVLNVNRVAVVCGTGLGTAQLIRIKIGKILGESVEVNTFSDIEAK
                     ANLDLLKYYDLVFTTVDFKTDLDVPIFRVNALFNEESIKKEIENAILLKNSINLKSIK
                     KIEKNIPFIGKLIEEDKFFILNKSTFMENLEDMLEKLVHKGIIDIEFKKKVLKREKKS
                     STALDNYIALPHSVNTNGENLYLAFGILEKPVIWDTKEIRIIILMIIPDKNVDSTDLI
                     IKSYEEVLEVGRDKKMVEELAKAKSFDEFYKIITKRR"
     gene            11075..11509
                     /locus_tag="PC414_002744"
     CDS             11075..11509
                     /locus_tag="PC414_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HDF2465132.1"
                     /translation="MDPVIFIIASLITVYLLNIFLGYLQLKDFNKNYIELKRKGRVAI
                     GRKKGRISSGTIVLILINDDGVIVETRKMQGVTVLARVKSFEGLVGKNLEYIEKSDLT
                     VYNKLLKKAILDAVKQYITFEKNKCEDTNNLSTDEECRMEVV"
     gene            11538..12098
                     /gene="srlA"
                     /locus_tag="PC414_002745"
     CDS             11538..12098
                     /gene="srlA"
                     /locus_tag="PC414_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418408.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDF2465133.1"
                     /translation="MTQFFQVLASGADSFMNLFRAGGQQFVGFVTDIVPLLVALLVTM
                     NAIINFIGTDRVERLAKKCSANIFTRYLILPVLGTFVFANPMTLSLGRFLPEKFKPSY
                     FAAASFSCHTMNGLFPHINPGELFIYLGIANGITTLGFSTADLAVRYLLVGIVANFIK
                     GVVTDYTTKFVEKQQGIALNSDIKIG"
     gene            12162..12530
                     /locus_tag="PC414_002746"
     CDS             12162..12530
                     /locus_tag="PC414_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIB"
                     /protein_id="HDF2465134.1"
                     /translation="MAFNKVKIVKGSGGWGGPLIIEPTEKKNKVVYVTGGAKPETAVK
                     IAELTGCELIDGFSQGVKDDEIACVIINCGGTLRCGIYPQKKIPTINIMKTGKSGPLA
                     MFIKEDIYVSGVKPTNVEQL"
     gene            12555..13121
                     /locus_tag="PC414_002747"
     CDS             12555..13121
                     /locus_tag="PC414_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDF2465135.1"
                     /translation="MAYDTKKKISEQSQGIVAKIGIALGNVTSTLFQAGRDTIDTLIH
                     TILPFMAFVAVLIGIINQSGIGNWVANLLTPLIGNVGGLVVLSIICSFPLLSPFLGPG
                     AVIAQIVGVLVGTEIGKGNIPPSLALPALFAINSQAACDFIPVGLGLAEAEPDTVQVG
                     VPSVLYSRFLTGAPTVLLAWLASFGLYK"
     gene            13162..13527
                     /locus_tag="PC414_002748"
     CDS             13162..13527
                     /locus_tag="PC414_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDF2465136.1"
                     /translation="MELIYSTQINKIGDNANEFLQHNMFVTFKDNAPQELENYCYIHS
                     ENNLVKEILTDDVLSINNVDYKITSVGELVNQNLSELGHITFKFTGEKDGAIGGTLYL
                     EEKEIAPIGVGTILKIIRK"
     gene            13628..14401
                     /gene="srlD"
                     /locus_tag="PC414_002749"
     CDS             13628..14401
                     /gene="srlD"
                     /locus_tag="PC414_002749"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HDF2465137.1"
                     /translation="MRKVALVVGGGRSLGEYLSKHLAKVGYDVAIADLNEDNAKKVAE
                     EIKTEFGCKSVGYSCNAIDERQVISTVKAIENDFDRIDLLVYNAGVAVSKKITDFGYK
                     DFKFIVDVNLNGYFLFAREVSKVMIAKGIEGRIIQINSKSGRVGSKHNSGYSAGKFAG
                     VGLTQSLALDLAEHKITVNALMLGNLLDSDMFESLIPQYAVKLGIPESEVKQVYIDKV
                     PLKRGCRFIDVANMLTFYASKEAEYCTGQSINITGGQVM"
     gene            14750..16894
                     /locus_tag="PC414_002750"
     CDS             14750..16894
                     /locus_tag="PC414_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF2465138.1"
                     /translation="MDIIKKLSHGCTLDCHDCCKFNVYTKGNNVVKIEGDKNHPYTKG
                     FICKKGMAHLDRLNHKDRIKTPMLKVDGVWKEISFDKAIEIMAEKLTYYKEKYTSKSV
                     MHYDQYGSGSVLKYIGDIFFNFYGGVSRHKGGPCWSAGMHAQKYDFGVAKSHAIEDML
                     NSKSIFVWGKNPAYTTIHTMQIIKKAKEKGIKIVVIDPIYTKTAQIADKYVQVNPGTD
                     GALAIAMAKIIVEDKLYDEEYINSYVIGFEEYKKYLSSLELSFLIDECGVKENDIREL
                     VDLYTNKYSSINVGYGLQKYKNGGNTIRAIDALGAITGQIGFSGGGVNYANKVYPSVI
                     NSDPYNSQSYGEDREFYVSNISKFIEESLKNTSNKVNYASDELDMTSNKVNYVSDELD
                     ITSNKTDYISNELYNLSNKSIKGNIPIKMAVITKSNMLNQLPDLVELERVFSKIEFKV
                     CFDMFITDTATLCDMFIPCTNTLESEDIIYSSMTNPYITYNERAVKPAHKLMDEYYFF
                     MELAKKMGLNDYPFVEKRTYLEKVIEPLKRFDKNLDIEKLKNNYFTIHNPVAWEDKKF
                     ETPSGKYELYSESIKNLGISPTPVYISNKYKEIEDKNISFRLLTNHHADTLFSQHFMD
                     KKSIAQAYINRRMAKKVGIEDKDIVILRSKKAKINVQINIDDGVGNYIVKMYVGWWKK
                     HGNPNSLTDTGISDFGGQVTYNESMVEIIRQN"
     gene            16982..17317
                     /gene="spoIIAA"
                     /locus_tag="PC414_002751"
     CDS             16982..17317
                     /gene="spoIIAA"
                     /locus_tag="PC414_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor antagonist"
                     /protein_id="HDF2465139.1"
                     /translation="MVNYSLEHKNLYIEFMCSELDHHVANEIREEIDNLLSVNQVKNV
                     VFNFENINFMDSSGIGVIIGRYKKISNEGGRVSVINISSRVKKIFDLSGLNKIIGIYD
                     TYEEALSSL"
     gene            17331..17759
                     /locus_tag="PC414_002752"
     CDS             17331..17759
                     /locus_tag="PC414_002752"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HDF2465140.1"
                     /translation="MNNIMEVKFSAISENESLARVIVASFAAKLDPTVDELVDIKTAV
                     SEAVTNAIIHGYEEDSSKFVFLRCEIEGNTIKIIVEDEGYGIENVEKAMEPLYTSKPE
                     LDRSGMGFTVMKSFMDDVEVSSVKDNGTRIEMTKKINVPK"
     gene            17782..18546
                     /gene="sigF"
                     /locus_tag="PC414_002753"
     CDS             17782..18546
                     /gene="sigF"
                     /locus_tag="PC414_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigF"
                     /protein_id="HDF2465141.1"
                     /translation="MEVTVAREEKKPLLSHEETLELIEKVQNGDEEAKEILISSNLGL
                     VRSVVSRFLNIGYDREDLFQLGSIGLIKSIYKFDPKFNVKFSTYAVPMILGEIKRYLR
                     DDGMIKVSRSLKQIAIKAKMESEALTKKLGREPSIEELAKAIDVEKEDLVMAMEANFN
                     VEYLQGVIHEEEGSPICLIDKISMKGESEEEKVVDNILLKDILGRLDKRERQIIVLRY
                     FEDMTQSEIGEMLNISQVQVSRIEKKVLSKLKEYIS"
BASE COUNT         7237 a         2102 c         3203 g         6034 t
ORIGIN      
        1 atggatgtaa taattaaaat aaaaaatatt attttatatt tataaacatt gacattctaa
       61 ttttgtagtg taatcgtatt ttttattaat tcttacaaaa tcaatgaaaa taagtatatc
      121 cattttaaag tacataaatt taaaaatcca gaaaaatata cttaaattat gtaagtaaaa
      181 gaggggttga atcagttgaa aatgtaatat tagtaaatag tacatcaatc ctatataccc
      241 tgtaagcaac tctattttca tgtactaaaa atgtacctat tttattaact gaaaagaata
      301 aattaaatgt aagtacataa gaagatagaa aaaattaaaa acaaaaaata tttatataaa
      361 taaatataaa aaatgatata atccctatta aaaaatttat agatgatgaa gaaatatcaa
      421 aatcttatat tataggaaga gacacttctt tgccaaaatc tatagaaaag caataccaaa
      481 tccaaagaga ataggcgaag aaaaaaataa aataaattca agagtaatag aaaagtttta
      541 tagaataaag aactgataac tctttatata acgaaaaata gaattttaag agaaaatgat
      601 ttaataaatg atttaacatg gtgttttagc aggtaaaaat aaatcaccag taatgattat
      661 agaaaaatta ctttatgaat ctcaaaaaac ttagttaaga gtaagagatt tgataagata
      721 actaaagttg gggaaatgtg tttgatgaga caaaaaatta gtaaaatagt ataatctaaa
      781 taaggatatt ttgttgatta aactacacta atagtaatat tgtaggggga aaagatgaga
      841 gaagaatcga actctttaag tttagaccat atagaaattt tctttgaact tttatctcaa
      901 agtacagaag attatatatt tttttgggat ataaatagga ataaatttaa aatttcttca
      961 gcaatttttg atgaatttaa cttaagtaaa gagatagaat ctgatttggt caattgttgg
     1021 agtaaaattg tttatccgga tgatgttcaa atatggaaag atgatataca agaattacta
     1081 catggaaaaa aaggtgagca caatttagaa tataggttga ttaataaata taaagaaata
     1141 gtatggattt cttgtagagg taaagtttat gtgtcggatg acccaaaaac tatatttcta
     1201 gtcgggagaa taaagaatat tggtgaaaaa aataaatttg attctatcac aggaacgtgg
     1261 aatagagaac aatttgaaca tagaatgaat tatcttataa aagagaaaat ttataaaaat
     1321 ggtgctatgt ttattatgga tattgataat tttaaaaata taaatgaaaa atatggacat
     1381 tcttatggtg acaaagtatt gcgtgcaatt gctactgaag tattagaata tcttccaaaa
     1441 gatgtaagat tgtataggtt agatggagat gaatttgcat ttttttaccc tatgtgtact
     1501 aaagaaacta tagagaaaat atatgaaaag atacagatgt atacaaatac tcagcatgaa
     1561 attgaatcaa ataagtacta ttgcacagtg actgcaggcg ttgctatgta tccagaagat
     1621 ggagataatt atttagattt atttaaacat gctgatattg cattggatat agcaaaaatt
     1681 agtggtaaaa atagaattaa gtttttttca caagagctct atgaaaataa actaaaggta
     1741 atttcaatgc aacaaaaact aagagagtgc gttgaaaata attttaatga ttttgagttg
     1801 ttttttcaac cccaggtaaa tgctgttaca aaagaggtaa taggggcaga ggtactttta
     1861 agatggcatt cgagtactta tggagaagta tctcctgttg aatttatacc aatactagaa
     1921 cagagtaatt taattatacc agttgggaag tggattataa aagaagctgt aaaacagtgt
     1981 aaagaatggc ataaaataaa tccagacttt aaaatatctg taaatgtatc gtatattcag
     2041 ttaaaggaag atttttttag agattttata gtagaatgtt tagtagagta tcaattaaga
     2101 cctgaatttt taattttgga attaacagag aattgttgga taccagatat aaatttacta
     2161 aatgataaat ttattagttt aaagggtata ggagtatata ttgcaataga tgactttggt
     2221 acaggatatt catctttaaa ttaccttaaa gaactgtctg ttaatataat aaaaattgaa
     2281 agaagttttg ttaagaatat tacatataat agttatgaat atacttttct tgaatatatt
     2341 attaaattag ctcatattat aaatttaaaa gtttgtgttg agggaataga gtcttatgaa
     2401 gagtatgata tagtaaaaag cttaggtgta gatattattc agggtttttt atttggaaga
     2461 cctgtaagtg cctctgaatt ttataaattt cagctatcaa agtgatgtat ataaaatgag
     2521 atatatagta tataaaaata gggcaaagtg gaagttttga agtttattaa aaatttacac
     2581 tttaccctat tttgttatgt cttctataaa ggattaatat actacactaa ctattatata
     2641 ctttcactcc aaatttagag attatttcac tagctttttg gacatcttcc ttagtggcat
     2701 caggaacatc ttttagttca taatcgatac ctaaactttc ccatttgtgt attcccatag
     2761 aatggtatgg tagcaattca aatctttcta catttttcaa tgtagctaca aattgaccta
     2821 gtttttctaa attatctata tcatcagtaa tgccaggaac taaaacatgt cttatccata
     2881 caggaatatt tcttgattct aagtgtctag caagttttaa tgccttatcc ataccaacac
     2941 cagtcaaatc aatagatttt tcctcaacca tatgttttaa atcaagtaaa accaaatcaa
     3001 tattatctaa aactgggtct atagtttcta tatctacaaa tccagatgta tccaagcaag
     3061 tgtgtatatc attttctttt gcttttttaa ataattcact taaaaattct ggttgaagag
     3121 tggattcacc accagaggca gttatacctc caccagaaaa cttcataaaa gaagtatact
     3181 taagagcatc cgttattatt tcatcaactg tgtattcttt tcctgattta atatcccatg
     3241 tatccctgtt atgacagtat ttgcatctaa gaggacaccc ctgaaaaaat aatatatatc
     3301 ttattcctgg tccatctaca gtaccaaacg tttctataga atgtatcttt cccttaatca
     3361 tagagttcac ctcacaatat attttaataa ccttatcaat agttataata tttctctaaa
     3421 aaaggccatc ttataccaga aataattcaa atataagata gccttttatt tttatttttt
     3481 acacagtagt aatcttagtt aaagtataaa aatttaaaga ctaagccatt ttaccgtgga
     3541 atgttctgtt tataacatct aattgttgct ctttagttaa ttttatgaag ttaactgcat
     3601 atccagatac tcttatagtt aattgtggat attcttctgg atgttccata gcatcttcta
     3661 aagtagctct atcaaataca tttacattta agtgatgagc attttgagcg aagtatccat
     3721 ccatcattgc agaaagattg tttattcttt cagtcatatc ttttcctaaa gcaccaggta
     3781 ctatagagaa agtattagat ataccatctt gagcatgttc atatggtaat ttagcaactg
     3841 aagataatga agctaaagca ccattagtat ctctaccatg cattgggtta gcaccaggag
     3901 caaatggagc accagctctt ctaccatctg gtgtattacc agttttctta ccataaacaa
     3961 catttgaagt tattgtaagt atagattgag ttggataaga atttctataa gttttattct
     4021 ttcttatttt attcataaag ctttcaacta actctacagc tatactatca actctatcat
     4081 cattatttcc gtattttgga taatctcctt ctatttcaaa atcaacagct attccttgtt
     4141 catttctaat agttttaacc ttagcatgtt ttatagcaga taatgagtct gcacaaactg
     4201 ataacccagc cataccacaa gccatagttc taaatatatc tctatcatgt aatgccattt
     4261 ctaatgcttc ataagaatat ttatcatgca tataatgtat tacatttaaa gtatttacat
     4321 ataatgttgc taaccaatca gtgaatggtt caaatttact cataacttca tcataatcta
     4381 agtactcaga agttataggt tcaaatcttg gtccaacttg aacaccagat ttctcatcta
     4441 caccaccatt tatagcgtat aataaagttt tagctaagtt aactctagct ccaaagaact
     4501 gcatttgttt accaattctc attgcagata cacaacaagc tataccatag tcatctcccc
     4561 agtatgctct cattaaatca tcattttcat actgtactga acttgtatct atagatactt
     4621 tagaacagaa atctttgaat ccttgaggta atttagttga ccatagaact gttaagtttg
     4681 gttctggaga aggtcctaat gtatatagag tatttaacat tctaaatgag tttttagtaa
     4741 ctaatgttct accatctatt cccatacctc ctatacactc agttacccaa gttgggtctc
     4801 cagagaatag gttattgtaa tcaggagttc ttaggaactt aaccattctt aatttcataa
     4861 caaaatggtc catatactct tgtaattctt cttctgttac gattccagct tttaaatctc
     4921 tttcaaagta tatatctaag aaagtagatg ttctacctaa agacattgca gcaccatttt
     4981 ggtctttaac agcacctaaa taaccaaagt ataaccattg tacagcttct tttgagttag
     5041 ttgctggttt tgatatatca aatccataag attcagccat ttttttaagt tcatttaaag
     5101 ctgatatttg ctcagttatt tcttctctta atctgataac ttcctcatcc atgcaagaaa
     5161 cttctaatga tttcttttgc tcatttttat cttctattaa agcatcaaca ccgtatagag
     5221 caactcttct gtagtcacca attattctac ctctaccata agcatctgga agacctgtta
     5281 ttataccaga ttttctagca gctctcattt ctggagtata aacatcaaaa actccttggt
     5341 tgtgtgtttt tctgtattta gtaaatatat cttttatttc tgggtctaat tcatatccaa
     5401 aagcttcaca tgaattttca accatcttta taccaccata tggcatgaca gctcttttta
     5461 aaggggcatc cgtttgtaac ccaactattg tttctaattc tttatctaaa tatcctggag
     5521 catatgcagc tattccagaa acagtttttg tatcaacatc taaagtacca ccattttctc
     5581 tttctttttt aaataggacc attgcttcgt cccataattt tttagtattt tcagtggcac
     5641 cagctaagaa agagtcattt ccctcatatg gagaatagtt taattgtata aactctctaa
     5701 catttatttc tttagtccat cttcctgttt taaatccttg ccatgcattc attaagtatt
     5761 tcctccttag atttaaatga taaataatac tattaaaatt aatttttata tacttaattt
     5821 attttatata tttttttaaa aatatatttt ctaatataat aatagcatat tattcgtatc
     5881 ctgtatacat aaaaaataaa aaattgtcgc tttaaagtat aaaacagatt atatgttaat
     5941 aatgtggtga aattataccc atatataaat aaaataaaac aaataaatag ctaattaagg
     6001 agaaaatatt ttttattttt tgtttaggat aaataaattt tattatggaa aaaagaaaaa
     6061 gcatacatct tcacaaactg aatatttatt taataggggg aggtgataga ttgtttatca
     6121 cagtggtatt atttttagta ggtttcctat taattactaa aggcgcagat atctttataa
     6181 attgtactgt agagataggt aaaaaaacga atatatcaga aattatactt ggagctacta
     6241 tagttagttt tgcaacaaca ttacctgagt ttacagtgtc tctacttgct tctattgatg
     6301 gtcatactac tatgagttta ggaaatgcag ttggttctat aatatgtaat acagggttag
     6361 cattagggtt ggttgttttt ataagtccat ttaatgtaga taaaaaaatg tttttttcta
     6421 agtctttact attaatagta tccgttatag ttttaatttt attgagttta gatggagtta
     6481 taacaagagg tgattcatta ttacttataa taatattaat cttttatatg gttaataatt
     6541 atagaagtgt cgttggaaaa tctgatacta aaaatagaaa tattaaaaat aacactatta
     6601 aagacatatc tactaaaaaa gggaaaaatt atagaggttt ttccgtatta gaaattgtaa
     6661 aaatactatt actttttgct acaggattga taatgatgat aataggttca caaatcctta
     6721 tagagagtgg agtaataata gctagttttt taaatatacc tcaaggtata gtaagtttaa
     6781 ctataatagc acttggaact tctctgccag agatagtttc gtcgattact gctataagaa
     6841 aaaatcatca tgaaatatca gtaggaaata ttttgggtgc aaacatattg aatatagtat
     6901 cagtaatagc tgtatcagca ataccaaata acataccaat actatctcaa aatagacaat
     6961 tggatatacc attcatgata ttattattat taatagtaat aataccaaca cttaaatcta
     7021 ataaacttag tagaatacaa ggtatattga tgttattcac atattttctg tacatatcaa
     7081 ttttgtattt catgtatatt atttaactat actgtttaaa atattaattg cagaatatcc
     7141 taaaaaagta tataatggat aggttaagat taattgataa cggagaggaa taatatatga
     7201 atataacaaa gtttgaagat ttaccaataa gtgagggaat aaaaaaagct atagctgaaa
     7261 tggggtttga agaaccatct cctatacaag cacagtctat acctgcgatt ttgtcaggta
     7321 aagacgttat aggacaagct caaactggta cgggaaaaac agctgctttt agtatcccta
     7381 tactagaaac aatagaccca aataatagaa gtttacaagc tgtagtgctt tgtccaacaa
     7441 gagaactggc aatacaagta tcaacagaaa taagaaagtt agctaagtat tcgcacggaa
     7501 taaagacatt acctatatat ggtggtcagc caatagatag acaaataaaa tctctaaaga
     7561 gtggtgtgca agttgtaata ggtacaccag gacgtacaat agaccatatc aatcgtaaaa
     7621 cattaaagat ggataatgta aaaatgatta tattagatga agcagatgaa atgctagata
     7681 tgggatttag agaagatata gaaatgattt taagtaagat tccagaagaa agacaaacaa
     7741 ctttcttttc agctactatg ccaagaggaa ttttggaatt aactaagaga tatcaaaaag
     7801 acccagagca tataaaagtt gttagaaaag aacttacagt ttcaaataca aagcaatact
     7861 atatagaaac taggtcatca aataaattag aagtattatg tagattagta gatgtatatg
     7921 accctaaact atctgttgtt ttctgtaata ctaagagaaa agcagacgaa ttggtaggag
     7981 atttacaagc aagaggatat tttgcagatg cattacatgg tgacttaaaa cagactcaaa
     8041 gagatatagt aatggataag tttagaaatg gtacaataga tatacttgtt gctactgatg
     8101 ttgcagctag aggtatagat gttgatgatg ttgaatgtgt attcaactat gacttaccac
     8161 aagatgaaga atattacgtt catagaattg gtagaactgg tcgtgctggt agagaaggta
     8221 tgtcatttac ttttgtattt ggaaaagaaa tgagaaagat gaaagatata gaaagatata
     8281 ctaaatctaa actaataaag cataatatac caacaataac tgatgttgaa gagaaaaaag
     8341 taggtacttt ctttgcacaa gttaaacaaa ctatagaaga aggtcactta actaaacaat
     8401 tacaatggtt agaaggtttc tgtaatgatg aagattatgc aatggttgat atagctgctg
     8461 cacttgtaaa actatctcta ggtgaagaaa tgaaagaaga aataatagaa gaaaaaccta
     8521 gaagagagcg tggagataga aaaggtggta ctggagctaa agatggtatg attagattat
     8581 ttatcaatat tggtagaaat caaagagtac aagcaaaaga tatagttggt gccatagctg
     8641 gagaagttgg aataccaggt aaagtagtag gtactataga tatatatgat aagtatactt
     8701 ttgttgaaat acctaaaaaa gatgcaaaaa cagtaattga aaagatgaaa gatattaaga
     8761 taaaaggtaa taagattaat atagaaaaag caaataagaa gaaaaaataa cgtatagtat
     8821 acaaaactat aattagaaaa tatataaaat aatatattgg atatttaaaa agcctgatgt
     8881 gttttcaaaa cagatagtag ataatctgta agagttaaca gcataaggct tttcttgttt
     8941 tatatgatgt tataattttc cagattgttg tgaaaaatgt atattagttc ctttttttca
     9001 aatctgattt gaaaaagaga atattaatta tctatatagt ttagattata ataaaaaatg
     9061 taagataaca aaggggagaa aaagatgtca atgacttata tggaccctag ggtttttttg
     9121 attttagaaa tggtgttaga agaagacaca aatactctag aacaattttc aaatgttttg
     9181 ggtgttagta ctaggactat cagaaactat ataaaccaga ttaatcgaga attaaaaggt
     9241 ggtattgcta aaatagttaa aaatgaaaaa ggtacatata gtataagaat tgaggatgaa
     9301 agtaaattaa ttgagattgt aaattttaat agaggaaagc tatcaaattt tataaattta
     9361 aattcacctg atgaaagaat aaattatgtt cttgatattt taactatgac caacaatcca
     9421 attaccatag atgatttggc tgaagagata tgtataggaa gaacaacatt aataaaggat
     9481 ttaaaaaaag cagacagaat tttagctgaa tatgatttag aattaaaaag gaagccaaat
     9541 ataggtatga ttttagttgg tgaagaaatg gatataagat tattaatcct tgatagattg
     9601 tatgaaaatt atattgatgt cttggaaaac gttaatcaga taaatttagt taaagattat
     9661 gatattgaat gtcttagaga tgaattaaaa aagttattta aaaaagaaga actctacata
     9721 acagaacagg tcttgagaga tgttgaaaga tatataatca tgtctgtctt aagaaatcta
     9781 aatgggtaca aatttgaaaa gatagataag agatttgaag tgataagatg ttcagatgaa
     9841 tatactttag gattaaagct caaagcttta ttggaaaaga tatttaatat agttttaaat
     9901 gaaaaagaaa ccatattttt gacaatgcca ttaataggta gaaaagctcc tagtacaaaa
     9961 ttagcactta gcagtataaa gataaatgat agcgtaaagg aagttgtaga tgaagtaact
    10021 agttttttat tagagacctc agggattgat tttaaagaag ataaaaagct tatagaaaat
    10081 ttggaatatc acttgtattt tgcacttaat agaatgagat tcaacataag ggtaaaaaat
    10141 cctcttttac aagaaataaa ggaaagatat acatttccat atagtgttgc aaagatagct
    10201 gcaatgataa ttgaggaaaa gtttgacttg aaaatatgtg accacgaaat aggatatata
    10261 gcattacatt ttggaagtta ttttgaaaaa aattcaagaa aagttctaaa tgtaaatagg
    10321 gtagctgttg tttgtggaac aggtcttggg actgcacaac ttattagaat aaagatagga
    10381 aaaatacttg gagaaagtgt agaagtaaat actttttctg atatagaagc taaagctaat
    10441 ttagatttat taaagtatta tgatttagta tttacaacag tagatttcaa aacagactta
    10501 gatgttccaa tttttagagt aaatgcgtta tttaatgaag aaagtataaa gaaagagata
    10561 gaaaatgcaa tacttttgaa aaatagtata aatcttaagt ctataaaaaa gattgaaaaa
    10621 aatatccctt ttataggtaa attaattgaa gaggataagt tctttatatt gaataagagt
    10681 acgtttatgg aaaatttaga agatatgtta gaaaaattag ttcataaagg aattattgat
    10741 atagagttca aaaaaaaggt attaaaaaga gaaaagaaat cttcaacagc attagataat
    10801 tatatagcat tgcctcactc agttaatact aatggggaaa atctttatct ggcatttgga
    10861 atattagaaa aaccagtgat ttgggataca aaggaaatta gaataataat attgatgata
    10921 attccagata agaatgtaga tagtacagac ctgataataa aaagttatga agaagtactt
    10981 gaagtaggga gagataaaaa gatggtagaa gaacttgcta aggcaaaatc atttgatgaa
    11041 ttttataaaa taattaccaa gaggaggtaa taatatggac ccagtaattt ttattatagc
    11101 atcacttatt acagtgtatt tattaaatat ttttcttgga tacttacagc ttaaagattt
    11161 taataaaaat tatattgaac taaaaagaaa aggaagagtg gcaattggca gaaaaaaagg
    11221 aagaatttca tcgggtacaa ttgtgttgat acttataaat gatgatggtg tgattgtcga
    11281 gactagaaaa atgcaaggtg ttacagtatt ggcaagagta aagtcatttg aaggtttggt
    11341 aggaaagaac ttagagtata tagaaaaatc agatttaact gtttacaata aactacttaa
    11401 aaaagcaata ctagatgcag tcaaacagta cataacattt gagaaaaata aatgtgaaga
    11461 tacaaataat ctgagtactg atgaagagtg taggatggaa gtagtttaaa aaaatatatt
    11521 tataaggagg agttaatatg acgcaatttt ttcaagtatt agcaagtggt gcagatagtt
    11581 ttatgaattt atttagggca ggtgggcaac aatttgtagg ttttgtaaca gatatagtac
    11641 cacttttagt agcattatta gttacaatga atgcaattat caactttatt ggtacagata
    11701 gagtcgagag acttgcaaaa aaatgttctg caaatatatt tactagatat ttgatattac
    11761 cagtccttgg tacatttgta tttgcaaacc caatgacact atcattagga agattcttgc
    11821 cagaaaagtt taaacctagt tactttgcag cagcaagctt ttcatgccat acaatgaatg
    11881 gattattccc acatatcaat ccaggtgaac tatttatata tctaggaatt gcaaatggta
    11941 taacaacttt gggattctca acagcagatt tagcagttag atatttgtta gtgggtatag
    12001 ttgcaaactt tattaaaggt gtagttactg actatacaac aaaatttgta gagaaacaac
    12061 aaggaatagc attaaattca gatataaaga ttggataatt actttaaggt ataaataaat
    12121 ttttcaaata aaaagatagt gtatagagga ggataaatta tatggcattt aacaaagtta
    12181 aaatagtaaa aggttctgga ggatggggtg gaccattgat aatagaacca acagaaaaga
    12241 agaataaggt agtatatgtg actgggggag caaagccaga aactgcagta aaaatagctg
    12301 aacttacagg atgtgagtta atagatggat tttcacaggg tgttaaagat gatgaaatcg
    12361 catgtgtaat aataaattgt ggtggaacat tgagatgtgg tatatatcct caaaagaaaa
    12421 taccgactat aaatataatg aaaacaggaa aaagtggacc attagcaatg tttataaaag
    12481 aagatatata tgtttcaggg gtaaaaccaa caaatgtaga gcagttataa ttgaggtagt
    12541 aggagggaag aaatatggct tatgatacta aaaagaagat aagtgagcag agtcaaggaa
    12601 ttgttgcaaa aataggtata gcactaggaa atgtgacaag tactcttttt caagctggta
    12661 gggatactat agatacatta atacatacta tactgccttt tatggctttc gttgcagtcc
    12721 taataggtat aataaatcaa tcaggtatag gaaattgggt agccaattta cttactccat
    12781 taatcggaaa tgttggaggt ttagttgtct tatcaataat atgttcattt ccgttgttgt
    12841 ctccgtttct tggaccagga gctgttatag ctcagatagt gggagtattg gttggtacag
    12901 aaataggaaa aggaaatatt ccaccaagtt tagcattacc agcattgttt gcaataaact
    12961 cacaagcagc ttgtgacttt ataccagtag gtcttggtct tgcagaagct gaacctgata
    13021 cagttcaagt aggagttccg tcagttttat attctagatt tttaacaggt gcaccaactg
    13081 ttctattagc atggttagca agctttgggt tatataaata gaagaatact ttataaataa
    13141 ttagagagag gttcagttga tatggaatta atatacagta ctcaaataaa taaaatagga
    13201 gataatgcaa atgaattttt acaacataat atgtttgtaa ctttcaaaga taatgctcct
    13261 caagagttag aaaattactg ttatatacat tctgaaaata atttagtaaa agaaatactt
    13321 acggatgatg tgctatcaat aaataatgtt gattacaaaa ttacttctgt tggtgagtta
    13381 gtaaaccaaa atctttcaga attaggtcat ataacattta agtttacagg tgaaaaagat
    13441 ggagcaattg gaggaacctt atatcttgaa gaaaaagaaa tagcacctat tggcgtaggt
    13501 actatactaa aaataattag aaaataaaat ttaaaacgaa tctaatcata ttataagtaa
    13561 attatagata aaagtcaatt taactaatca agttaacttt taatgtgata aaaaagggga
    13621 aatgaaaatg agaaaagtgg ctttagtcgt tggaggagga agaagtttag gtgaatattt
    13681 atctaaacat ttagctaaag taggttatga tgttgctatt gcagatttaa atgaagacaa
    13741 cgcaaaaaaa gtagctgaag aaataaaaac tgaatttggt tgtaaatctg taggatatag
    13801 ttgtaatgcc atagatgaaa gacaggttat aagtactgtc aaagctattg aaaatgattt
    13861 tgatagaata gatttattag tgtacaatgc aggagtggct gtaagtaaaa aaataactga
    13921 ttttggatac aaagatttta aatttattgt agacgtaaat ttaaatgggt actttctatt
    13981 tgcaagagaa gtatctaaag tcatgatagc aaaaggtatt gagggtagaa taatacagat
    14041 aaattcaaaa tcaggtagag tgggaagcaa gcataattca ggatattcag caggtaaatt
    14101 tgctggagta ggacttactc aaagtttagc gctagatttg gcagaacata agataactgt
    14161 aaatgcactg atgctcggta atttacttga ttctgatatg tttgaaagtt taattcctca
    14221 atatgcagta aaattgggaa tacctgaatc agaagtcaag caagtttata tagataaagt
    14281 acctttaaaa agagggtgta gatttataga tgttgcaaat atgcttacat tttatgcttc
    14341 aaaggaagct gaatactgca ctggtcaatc tataaatatt actggtggac aagttatgta
    14401 aggtttttat ggagtgtctc aagatgaact ctttagttca tgaggcactt ttttgtataa
    14461 aattaaatat attttcagca tttaaacaat gaaaaagagg tttatcttta ttttgagata
    14521 gcctcatttt tgtaattaaa aaacatagtg tttaagtaaa catataagat tgttagtttt
    14581 ttaaaagaga attttttaat aatattatat ttatgaagta tttttaaact tattttgata
    14641 aaatatagct atgttttata tactgtagtt ttgaacatag aaagtttaat aaattatagt
    14701 atatatgtta atttttaata aggtaagaaa gtagaaagaa ggatatttta tggatataat
    14761 aaaaaaatta agtcatgggt gtacattaga ttgtcatgac tgttgtaaat tcaatgttta
    14821 tactaaagga aataatgtag taaaaattga aggagataaa aatcatcctt atacaaaagg
    14881 ttttatatgc aaaaaaggga tggcccattt agatagatta aatcataaag atagaataaa
    14941 gactcctatg cttaaagttg atggagtatg gaaagagatt tcttttgata aagctataga
    15001 aataatggca gaaaagctta catactataa agaaaagtat acatcaaagt ctgtcatgca
    15061 ttatgaccaa tatggaagtg ggtctgtgct aaaatacata ggagatatat tttttaattt
    15121 ttatggaggt gttagcagac ataaaggtgg tccctgttgg agtgcaggga tgcatgctca
    15181 aaaatatgat tttggagtag ctaagagtca tgctatagaa gatatgttaa atagtaaaag
    15241 tatatttgtg tggggaaaaa atccagcata tactacaatt catactatgc aaataattaa
    15301 aaaagctaaa gaaaaaggga taaagatagt agtgattgac cccatatata caaaaacagc
    15361 tcaaatagca gataagtatg tacaagtaaa tcctggaaca gatggagctt tagctatagc
    15421 tatggcaaaa attatagtag aagataaatt atatgatgaa gaatatataa actcgtatgt
    15481 tattggtttt gaagaatata aaaaatattt atcttcattg gaattaagtt ttttaattga
    15541 tgaatgtggt gttaaagaga atgatataag agagttagtg gatttatata ctaacaaata
    15601 ttcaagtata aatgttggat atgggcttca aaaatataaa aatggtggaa acacaataag
    15661 agcaatagat gctttaggtg ctataactgg tcaaatagga tttagtggtg gaggggtaaa
    15721 ctatgctaat aaagtttatc cgagtgttat aaattctgac ccttacaata gccaatcata
    15781 tggtgaagat agggagtttt atgtaagtaa tatcagtaaa tttatagaag aatctttaaa
    15841 aaatacatca aataaagtaa attatgcatc agatgaactt gatatgacat caaataaagt
    15901 aaattatgta tcagatgaac ttgatataac atcaaataaa acagattata tatcaaatga
    15961 attgtataat ttatctaata agtcaatcaa aggtaatatt cctataaaga tggctgtaat
    16021 aactaaaagt aatatgctga atcaactgcc agatttagta gaattggaga gggtgttttc
    16081 aaaaattgaa tttaaagtat gttttgatat gtttataact gatacagcta ctttatgtga
    16141 catgtttata ccgtgtacaa atacactaga gagtgaagat ataatttata gctcaatgac
    16201 caatccatat ataacctata atgaaagagc tgtaaagcca gcacataaat tgatggatga
    16261 atattacttc tttatggagc tagctaaaaa gatgggatta aatgattatc cttttgtaga
    16321 aaagagaact tatttagaaa aagtgattga accacttaag cgttttgaca aaaatctgga
    16381 tatagaaaaa cttaaaaaca attactttac tatacataac cctgttgctt gggaagataa
    16441 gaagtttgag actccatctg gaaaatacga gctttattca gaaagtataa aaaacttggg
    16501 aataagtcca acacctgtat atataagcaa taaatataaa gaaatagaag ataaaaatat
    16561 ttcttttaga ttattaacta atcatcatgc agatacttta tttagtcagc attttatgga
    16621 caaaaaatct atagctcaag catatataaa tcggagaatg gctaaaaaag taggtattga
    16681 ggacaaagat attgtgatat tgaggtcaaa aaaagcaaaa ataaatgtac aaataaatat
    16741 agatgatgga gtaggtaatt atatagtaaa aatgtatgtt ggttggtgga agaaacatgg
    16801 aaatcctaat agtttaacag acactggtat atcagatttt gggggtcagg taacatataa
    16861 tgaaagtatg gtagaaataa tacgacaaaa ctagttaaat ttcattgaag gaataaaaat
    16921 ataattatag aattgattaa aagttactat ttttacaaaa ctattgaagg agggattttt
    16981 gatggtaaat tattccttag aacataagaa tctatacata gaatttatgt gctcagaact
    17041 agaccaccat gtagccaatg aaataaggga agaaatagat aacttattaa gtgtaaatca
    17101 agtaaaaaat gttgtattta attttgaaaa tattaatttt atggattctt caggtatagg
    17161 cgtcatcata ggtagatata aaaagatttc taatgaaggg ggacgagttt cagtaattaa
    17221 tataagttca cgagttaaga agatttttga tttatctggg ttaaataaaa ttattggcat
    17281 ttatgatacg tatgaagaag ctctaagttc tttgtaggga ggagtatgtg atgaataata
    17341 ttatggaagt taaattttcg gcaatatctg aaaatgaaag tttagcaaga gttatagtag
    17401 catcctttgc agctaagtta gacccaacag tagatgagtt agtagatata aaaactgctg
    17461 tctctgaggc tgttacgaat gctataatac atggatatga agaagacagt tctaagtttg
    17521 ttttcttaag atgtgagata gaaggaaata ctataaagat tatagttgaa gatgaagggt
    17581 atggaataga aaatgtggag aaggcaatgg aaccacttta tacatcaaaa ccagaacttg
    17641 atagatcagg aatgggattc acagtaatga aaagctttat ggacgatgta gaggtaagtt
    17701 ctgtaaaaga taatgggact agaattgaaa tgactaaaaa aataaatgtg cctaaataaa
    17761 gtccaaaagt gaggttttat tatggaagta actgttgcca gagaagaaaa aaaacctctt
    17821 cttagtcatg aagagactct agaacttatt gaaaaagttc aaaatggaga tgaagaggca
    17881 aaagaaatat taatatcaag caatttaggt ctagttagga gcgttgtatc tagattttta
    17941 aatataggat atgatagaga agatttattt cagttaggtt ctataggctt aataaaatca
    18001 atatataaat ttgacccaaa atttaatgta aaattctcta cttatgcagt accaatgatt
    18061 ttaggtgaaa taaagagata cttaagagat gatggtatga taaaagtatc aaggtcgtta
    18121 aagcaaatag ctataaaggc aaagatggaa agtgaagctt taactaaaaa attaggtaga
    18181 gagccgagta tagaagaatt agctaaagca atagatgtag aaaaagaaga tttagtaatg
    18241 gctatggaag ctaatttcaa tgttgagtat ttgcaaggag ttattcatga agaggaaggt
    18301 tctccaatat gccttattga taaaataagc atgaaaggag aaagtgaaga agaaaaagta
    18361 gtggataaca tattgttaaa agacatactt ggaagactgg ataagagaga gcgtcaaata
    18421 atagttctca gatactttga agacatgact caaagtgaaa taggagagat gctcaatata
    18481 tcacaagttc aagtatctag gatagagaag aaagtattgt ctaagttaaa agaatatata
    18541 tcgtgataat acttatgata tgtagaataa taaaat
//
LOCUS       DAMJVK010000079        18089 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.080, whole genome shotgun sequence.
ACCESSION   DAMJVK010000079 DAMJVK010000000
VERSION     DAMJVK010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18089
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.080"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            575..1435
                     /locus_tag="PC414_002754"
     CDS             575..1435
                     /locus_tag="PC414_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2465142.1"
                     /translation="MKIIQILNNNVALVKRGKNEIIVFVKGIGFKKRVGQVINENEIE
                     RTYILDSYDMLEHFSYLLQNSDSNNIVLINEIIEYGEKTLNIKASDYLSLTLLDHLEF
                     LLKRCKKNQFIKSPLVWNVKRFYPKHFEIGMHALKLIGDSKGINLPDDEAVSIALHFV
                     NMEVNKESHDSTIVELRTLADIVSIIKYHFNVELDETSTNYMRFTTHLQYFIQRIMSE
                     EASIDEDNSIDLYKQVSQLYPKSFGSVQKIRKYIKSQFNVDISISEETYLMLHINRVT
                     QRLEAKSDEI"
     gene            1425..3296
                     /locus_tag="PC414_002755"
     CDS             1425..3296
                     /locus_tag="PC414_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2465143.1"
                     /translation="MKYKDLNENIIKLIGGKENIKSVAHCVTRLRFTLNDRSIAQTEE
                     IKKLKGVIDVVSNDVAYQIIIGTHVVDVYNEFMSMIGLSSSESSIKSERKKGIRGIKG
                     IFNSIFVVVSETMTSVIEVLLAAGILASILALLNMSGILQSDSPTYQILDTLRSATFH
                     FLPVLIAIASAKRLNVNPYIAVVLAVTLLSSSIDGVEGLSLFGINLQSTTYANSFIPI
                     LLGVWAMGIIIEGLKKILPKALHYFLVPVLSLIITLSVTLTVFGPIGMWIGDGLNYAC
                     MFLIDTLGNWSVVALYAALQPLLIVFGAANFTMPISLNSVTTLGYDPIFMGAATISDI
                     AVCGAMFGYFLKTRNKEQKQLFGTVSFSALMGVTEPSVFGVFMKYRRPFVAVMIGGGI
                     GGLIAGLAQVKTYGMVWGLAALPTYLVGGEVSNFTFMIISVVVSFVAATAASYLLGIP
                     SDEKEEVKGDNEKELELIINENNSRTISIGNVAKGQVIALGEVNDKAFSSGALGKGVG
                     VIPSESKSTVISPVDGTITIVFPTKHAYGIVTDEGLEILIHIGIDTVNLDGKFFESLV
                     TQNQRVKKGDALAVFESDKIIEEGFDSTIITVVTNTSDYLDVISSNDESEELLTVIV"
     gene            3316..4770
                     /locus_tag="PC414_002756"
     CDS             3316..4770
                     /locus_tag="PC414_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HDF2465144.1"
                     /translation="MNIKDTFFWGGSIAAHQCEGSWDSDNKGPAIMDFVTKGSYEMPR
                     VITDKIEEKLDYPSHNGIDFYNRYKEDIALFKEMGFSALRISIDWSRIFPNGDDENPN
                     ELGIKYYEGLIDTLIENNIEPIVTLYHFELPMNLVHKYGSWNNRKLIDLYLKYSETVI
                     RRFDDKVKYWVTFNEMNHIDPQTEHSDIFTYMIAGIKYSEIENKVQTLANVGYNMTLA
                     GVKVAKLAREINPNNKVGCVFGLNPIYPYNCNPSNVLNAFLDNDRDYYQIDAMCNGKF
                     PQYKLKEYEKNNITIEVTDEDKKAFKEGKLDFIGLNYYFSSVSTPKENLSGDKSLFGG
                     IQNPYLEQSKWGWAIDPIGLRFTLNYIYRKYQLPILITENGLGAYDKIEEDGTINDDY
                     RIEYLGKHLSQMKSAIEEDNVECFGYLMWGPIDLVSATTGQMSKRYGFIYVDLDDEGK
                     GSFKRYKKKSFDWYKEVIKSNGEVLDFSTDIVVG"
     gene            5795..7003
                     /locus_tag="PC414_002757"
     CDS             5795..7003
                     /locus_tag="PC414_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465145.1"
                     /translation="MENFWNPLTCFTVCVMIYAFSEFLSKKTKGAISTLLFVCIIFLL
                     GFWTNILPKDITGASGILAAMSNFGIALLITNLGTLINLEDLCHEWKTVVIALVGIVG
                     IGVGCLTVGSMLFGREYALIAAPPIAGSTVAGIIVTTVAEAANRPELAAFVILVLSFQ
                     KFFGIPITTYCINKELKFKRDNGDFLKDESKSNFKLPSMRIFKEDLGKKSSTLVYLAK
                     LSFVAMIGNFVGLATLIPGFTPANYYLNPNIACLLVGLIFTRIGFLEKGILDKSQSNG
                     IIMFGCMLMLPGGLAKVTPTALFGMIVPTIGILVVGSVFIILFTAIVGKLLGYSPRMS
                     AAIGVTCMLAYPATQIISNEGIDAMEGTEEEKQRATDYLLPKMIIGGFVTVTIASVAF
                     ASIIGPIIFG"
     gene            7034..8413
                     /locus_tag="PC414_002758"
     CDS             7034..8413
                     /locus_tag="PC414_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2465146.1"
                     /translation="MIGSISINEIDKKRFKLDDLSKKIWENPEKAFKEFKACENTANF
                     LRSEGFDVEVGVGGLATAIRASFGSGKPVIGFMGEFDALPGLNQKVSTKQEAFELGAY
                     GHGCGHNLLCTAHVGAVVGLKREMIENNLSGTIVFYACPGEEQLTGKGFMARGGAFEG
                     LDLAINFHPNKISEATIGTSTAVNSVKFHFKGKTAHAGSDPQNGRSALDAVELTNVGA
                     NYLREHVTSDVRIHYTITDGGVAPNIVPDKASVWYYTRALSREAVESTYERLVKVAKG
                     AAMMTETEVEVEFLGGCYNTLNNHVLANLVSECMNEIKQEPWTREELDFAAELDRQSP
                     QQYKAMISKYNLPEGTHLYYGGGNVTNFNSYGSTDIGDVMHIVPTAYFFTGCTNLGAP
                     GHSWQFTSCAGSSIGEKGMIYASKIMAMFGAKILNNPEIAKKAKEEFDKSMNGKTYKC
                     PIPDDIPTP"
     gene            8519..9709
                     /locus_tag="PC414_002759"
     CDS             8519..9709
                     /locus_tag="PC414_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2465147.1"
                     /translation="MKNFFKEAQSIEGEIIRWRRYLHENPEIGCNLPKTVTFVTSTLE
                     KMGYSPKLTSNNGIVAVLDSENGGKTLLLRADMDALPIREETDLPFKSKNKDASHSCG
                     HDNHTAMLLGAAKILMDNKHLLKGRVKFVFEPDEETTRGAKSMIDDGILENPQVDAAM
                     AFHSMVGKFLNTGQVAYSRGPAMASADIFEITVEGKATHGASPEFGVDPINIMSHIQI
                     ALNTILSREKPQKEPVVLTIGMFNAGNVPNAIPNKASMSGTLRTFDQFVREKLKNRII
                     EISTGIAKSFGGNAKVEFKMGTPAVINDLDVGDEIVSYVKEVIGNENVIDFPSAMGSE
                     DFSEVLLRVPGVFLWIGMGSISQGYNFGMHNPKAIFNEEGLVYGSALFAHCAVKWLEN
                     NFSR"
     gene            complement(9837..11165)
                     /locus_tag="PC414_002760"
     CDS             complement(9837..11165)
                     /locus_tag="PC414_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465148.1"
                     /translation="MMKIAVITTEFLKEFVDNSIKKLNINAEIEIYIYRDFSHVGDLY
                     LEIEDRFDGFAVSGPIPKKAITKKAGTIKKPLVDFGTDLQSYYDMFLKLIFKYNTLDF
                     SRAYFDLTDWADNPKDISYYLSNGTFGDLMDSIDENASKMSLSDIAKLEKKIAKKHIN
                     LWNEGKIDFSTTRFSSIVPMLQNAGVNFHFIYPDIDLLKNTFNNLIKDIHLHRMKGNQ
                     PSVIYITVKDTNYDDSTLDQVYKILNSIKKDKLTDFIIEKNSNSFKIFTKCETIKSLT
                     KNFTACYIKESIENSTNIKVCIGYGIGNDIKQAMSSAISANKESKINPLNYSFLVNEN
                     YETIGPLLCDKLLTVSNKVTPHINTIAKSSKLSTLTIQKIISVTEILQRDEVTPKELS
                     SCLNVTVRTANRFLSSLLKSGNATILYEKQNSTKGRPERVYKLFISENKY"
     gene            complement(11783..12997)
                     /locus_tag="PC414_002761"
     CDS             complement(11783..12997)
                     /locus_tag="PC414_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDF2465149.1"
                     /translation="MRNCIIAQSGGPTAVINASAVGIYEKNLETKYFDKVYFGINGIE
                     GILNKNIIDSDSLNKDNVLNLKQTPSSGLGSCRYKLADYKINSYDYDRVINILDEYKI
                     DTLFYIGGNDSMDTVMKFSQYCKNTTCKIKFIGIPKTIDNDLLGTDNCPGFGSAAKLI
                     STLIMETYLDFSIYKNNGIFIVETMGRDTGWLAASSSLARLNDTPLVDLIYLPEIDFY
                     EDKFIEDVRKIFKEKNKAYIVASEGIRDKYGNFICEQKTSAHDKFGHSQLGGVSNYLK
                     NLIVSSSITTRVKTLELGVIQRCAMHLASDTDLKQAAEVGAYGIELSKKGYTGVMAII
                     NRTCNNPYKYEVSHTDISKIANKTKYFPKEWINEECNYITDEALNYLYPLIQGKPDIK
                     YKNGLPYFSIIY"
     gene            13423..14139
                     /locus_tag="PC414_002762"
     CDS             13423..14139
                     /locus_tag="PC414_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentose-5-phosphate 3-epimerase"
                     /protein_id="HDF2465150.1"
                     /translation="MKHIKIAAGLAHVNYGHISAVVKEAMDAGVDYVHSDAADMHDLK
                     NMQLMGGHQIIEAIRPVTDKPIECHIYTRDCDRLFIEKIAAAGCNLLIIPAEHFLGAP
                     LAYIINYCREFGMKVGLTIGCYTPLCFVDEAIYDIDRLQIVVHGVDETDGKDNWGWRK
                     SAVDLVKRARKMIDEKNPKCELAIDGGLRADNMEPLIECNPDVVILSSAIFKDKDGIT
                     AGVKNCRKAIDEAATKFGLE"
     gene            14274..14900
                     /locus_tag="PC414_002763"
     CDS             14274..14900
                     /locus_tag="PC414_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF2465151.1"
                     /translation="MKKEMIKDYLLITISAFLMALAVNFFFAEHTLAPGGITGLSVVL
                     STFLSMPVENISLGISMPLLVMGLLFLGKGFGIKTLYITLMGPLFLKVIPQTHITDNL
                     LVAGVIGGLLVGTAIGIAIIRGCSTGGTDLVAMLINKVIKFLKLPVILFMLDGFIIVA
                     SGIISKNFMISIYSLISLLLIIKTIGFVTTKFGKNTEEINNINNACAQ"
     gene            complement(15100..16644)
                     /locus_tag="PC414_002764"
     CDS             complement(15100..16644)
                     /locus_tag="PC414_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2465152.1"
                     /translation="MNKRLLDIAELLLNSSDYITVDTIAEELKVSNKTIRNDLVILDE
                     WLLEFNLSLDKKTGSGVIILGNEDIKLKVIRDINDKSNCIYAYSPEDRKRYILSKLFI
                     KNSKFRIRDICNELHVSRATVHKDLVVIQNFLQNFKVTLVRKTNNGVYLEGKEKDIRK
                     AIFELATVNKSYTELKEILFSKSNECKDTPSTKIFKELFNYDFEKLSQITLHTLQVEK
                     SNLSDEYYINFLIHIAICIKRISIKRYINLSEGFFRELLEHEYFEKSKILCANLSEAF
                     DVEFIDEEVCYILLHIEGLLKSIKKTTTLEPLKNEEITEYNLSEAIAVHWGSILNLNL
                     KDDLILIKSLANHLKSVLHRINYGFTITNPILNDIKRTFPYTYKAAKESNTVIKQLMN
                     YEVNDDEIGYLALYLISAIDRSKSPLNTILICHCSDGVSNLLVQKLSFEFNQINIVKS
                     IPLSSISFTNFDDVDLILTTAPVDFEHNAELININALLSKNDISRLSTIIKKLYSEKN
                     KILSYK"
     gene            complement(16670..17311)
                     /locus_tag="PC414_002765"
     CDS             complement(16670..17311)
                     /locus_tag="PC414_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HDF2465153.1"
                     /translation="MKYLIDSANLDEIQKCLKYGFKGITANPSMYLKEKVNFHEFIET
                     CANLNPEILTAEVIGNNLYELINSCDKILNINKNTIIKINFSEVGLELINILNSKGVK
                     TACTLIFNVNQASLAINAGADYLFPFIGRNDENGNDGIKNLTEICNLISINGYNTKVV
                     AASIKNVFHLTQASLCGCDYAAVTYDLFKKASFNQLTIDGANTFERDWSKVNK"
     gene            17581..17772
                     /locus_tag="PC414_002766"
                     /pseudo
     CDS             17581..17772
                     /locus_tag="PC414_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422425.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
BASE COUNT         6698 a         2132 c         2984 g         6275 t
ORIGIN      
        1 aatttaagta tttatatatt agtatatctt actagcattt aaatgaaact aaatttaagt
       61 atttatatat tagtatatct tactagcatt taaatgaaac taaatttgaa gctttaccta
      121 ttaatatatc ttaatagtga ttaaataaaa ctaaacttaa gactttatat gttaatatat
      181 cttaagaatt tatttgttaa aatacagttc attatattta aaagcattac tatgataatt
      241 cataatagta aaatagataa tggttcagac aatattataa atactagtga cggtaaaata
      301 gtatcaattt agtatataaa ccttgtcaga taatcgcata acagagcttg tagttataga
      361 ataaaaaaat atctaggaaa ccgttgacag atttatatgg atagtataac atatagaata
      421 tgaaattaaa caaaaaagaa ttttaattat ggcgacgtag tcataatttt gagtatgtta
      481 ctgattagca ggcagaactc aagtatatat gtgaaaaata gagggatact ctatacatat
      541 atgcttgagt tctttttatt gaagggagga atatatgaaa ataattcaga ttttaaataa
      601 taatgtagcc ttagtaaaaa gaggtaaaaa tgaaattatt gtatttgtta aaggaattgg
      661 ctttaaaaaa agagtgggac aagtaataaa tgaaaatgaa atagagagaa catatatact
      721 agattcatac gatatgctag aacactttag ttatctacta caaaactcag actccaataa
      781 tatagttctt attaatgaaa taattgaata tggtgagaaa actttaaata taaaggcaag
      841 tgattatcta agcttaacct tactagacca tttagagttt ttacttaaaa gatgtaaaaa
      901 gaatcaattt ataaaaagtc ctttggtatg gaatgttaaa agattttatc caaagcattt
      961 tgaaattgga atgcatgcac taaaattaat tggtgatagt aagggaatta atcttcctga
     1021 tgatgaggca gtatcaatag cacttcattt tgtaaatatg gaggtaaata aagagtctca
     1081 tgatagtaca attgtagagt tgagaacttt agcagatata gtttctataa taaaatatca
     1141 ttttaatgta gagctagatg aaacttcaac aaattatatg agatttacta ctcatctaca
     1201 gtactttatc caaagaatta tgtcagagga agcttcaata gatgaagata attctataga
     1261 tttatataaa caagtaagtc aactttatcc aaagtcattt ggaagtgttc aaaagataag
     1321 aaaatatata aaatcacaat ttaatgtcga tatatctata agtgaagaaa cttatttaat
     1381 gttgcatata aatagggtga ctcaaagact ggaggcaaaa tcagatgaaa tataaagatt
     1441 taaatgaaaa tataataaaa cttatagggg gaaaagaaaa tataaagtcg gtagcgcatt
     1501 gtgttactcg tttaagattt actttaaatg ataggagtat cgctcaaaca gaagaaatta
     1561 aaaaattaaa aggagtaatt gatgtagttt ctaatgatgt tgcttatcaa attataatag
     1621 gtactcatgt tgtagatgta tataacgagt ttatgtctat gataggttta agttcaagtg
     1681 aatcaagtat aaaaagtgaa agaaaaaagg gaattagagg tatcaaaggt atatttaatt
     1741 ccatctttgt ggtagtctca gagactatga cttcagtaat tgaagtacta cttgcagctg
     1801 gtatacttgc aagtatatta gcacttttaa atatgtctgg catacttcag agtgacagtc
     1861 ctacatatca aatattagat acattaagaa gtgctacatt tcacttctta ccagtattaa
     1921 ttgcaatagc atcagcaaaa agacttaatg taaatccata tatagcagta gtattagcag
     1981 ttacgttatt atcttcatca atagatggtg tagaaggatt aagtttattt ggtataaatt
     2041 tacagtctac aacttatgca aacagtttta ttccaatact tttaggagta tgggcaatgg
     2101 gaattataat tgaaggtttg aaaaaaatat tacctaaagc actacactat tttttagttc
     2161 cagtattatc tttaataata actctatcag ttacattaac tgtatttgga cctattggta
     2221 tgtggattgg tgatggatta aactatgcat gtatgttttt aattgataca ttgggtaatt
     2281 ggagtgttgt tgcactgtat gcagctttac aaccactctt aatagtcttt ggtgcagcaa
     2341 actttactat gcctatatct ttaaattctg tgacaacatt ggggtatgac ccaattttta
     2401 tgggcgctgc aacaatatct gatattgcag tttgtggtgc aatgtttgga tatttcttaa
     2461 aaactaggaa taaagaacaa aaacaattat ttggtacagt aagttttagt gcacttatgg
     2521 gagttacaga accttcagtg tttggtgtgt ttatgaaata tagaagacca tttgtagcag
     2581 taatgatagg tggaggtata ggtggtttaa tagcaggttt agctcaagtt aaaacatatg
     2641 gaatggtttg gggattagct gcattaccaa cgtatcttgt tggaggagag gtatctaact
     2701 ttacatttat gataataagt gtagtagtat catttgtagc agcaacagca gcttcctatt
     2761 tactaggaat accttcagat gaaaaagaag aagttaaggg tgataatgaa aaagaattag
     2821 aactaataat caacgagaac aattcaagaa ctattagtat tggaaatgtt gcaaaaggtc
     2881 aggtaattgc acttggggaa gttaatgaca aagcattttc ctctggagca ttaggtaaag
     2941 gggttggagt tatacctagt gaatcaaaat caactgttat atctcctgta gatggaacta
     3001 taactatagt atttccaact aaacatgcat atggtatagt gactgatgaa ggattagaaa
     3061 tattaattca tattgggata gatactgtaa acttagatgg aaaattcttt gaaagtcttg
     3121 taacacaaaa tcaaagggtt aaaaaaggag atgctttagc agtatttgaa tctgataaaa
     3181 ttatagagga aggctttgat tctactataa taactgtagt tacaaatact agcgattatt
     3241 tagatgtaat ctcatcaaat gatgaatcag aagaattatt aactgtaata gtataaattt
     3301 taggagggaa aattcatgaa tattaaagat acatttttct ggggaggaag tattgcagcc
     3361 catcaatgtg aaggttcatg ggatagtgac aacaaaggac ctgcaattat ggattttgta
     3421 actaagggct cttatgaaat gccaagagta attactgata aaatagaaga aaaattggat
     3481 tatccatctc ataatggaat agatttttat aatagatata aagaagatat agctttattt
     3541 aaagaaatgg gatttagtgc attaagaata tctatagatt ggtcaagaat ttttccaaat
     3601 ggagatgatg aaaatcctaa tgaattaggg attaaatact atgaaggtct gatagataca
     3661 ttaatagaaa ataatataga acctattgta acactatatc attttgaatt acctatgaat
     3721 ttagtacata aatatggttc atggaataac agaaaactta tagatttgta tttaaagtac
     3781 agtgagactg taataagaag atttgatgat aaggtaaagt actgggttac atttaatgag
     3841 atgaaccata ttgaccctca aacagagcac tcggatattt ttacttatat gatagcaggt
     3901 attaagtata gtgaaataga aaataaggtt caaacattgg caaatgtagg gtataatatg
     3961 actttggcgg gtgtaaaggt tgcaaagttg gctagagaaa tcaatcccaa caataaagtt
     4021 ggatgtgtat ttggactaaa tccaatttat ccatacaatt gtaatcctag taatgtgtta
     4081 aatgcatttt tagataatga ccgtgattat tatcaaattg atgcaatgtg caatgggaag
     4141 tttccacaat ataaattaaa agaatatgaa aaaaataata taactattga agtgactgat
     4201 gaagacaaaa aagcatttaa agaaggtaag ttagatttta taggacttaa ttattatttt
     4261 tcaagtgtat ctactccaaa agaaaattta tcaggtgata aatctttatt tggtgggatt
     4321 caaaatccat atttggaaca aagtaagtgg gggtgggcaa tagaccctat aggtttaaga
     4381 tttaccttga actatattta cagaaagtat caattaccta tcttaattac agaaaatggt
     4441 ttaggggcat atgataaaat cgaagaagat ggaactataa atgatgatta tagaattgaa
     4501 tatttaggta agcatctatc tcaaatgaag tctgcaattg aagaagataa tgttgaatgt
     4561 tttggatatt tgatgtgggg accaattgat ttagtcagtg caactacagg tcagatgagt
     4621 aagagatatg gatttatcta tgttgactta gatgatgaag gcaaaggaag ttttaagaga
     4681 tataagaaaa aatcatttga ttggtacaaa gaagtaataa aatctaatgg agaagtttta
     4741 gatttttcta cggatatagt agtagggtag gaataagata aatagaagtt aggaactaat
     4801 gttaattagt gtctagtaaa gcatttaaaa tagagtatag ggtctttaaa gtaaagtgtg
     4861 tattcttctt aaaagtaatg gcaactaaaa ttactataaa aaggagaata cacatttttt
     4921 atgtttatac ttaagtaaat atttagacat attcatagta taatttactc attatataaa
     4981 aatagtatga tttactcact atataacttt ataaattttc tggaaaaatg tcagaatata
     5041 ttgacaaaga aaagatttaa aattataatc tatattaaga atagtttgac aatataaaaa
     5101 caagtatttt tataaatagt gaaattattt ttattgtcaa tttaagaata atttaggact
     5161 actataaaga taaaggtata gatgtcatta tatgataaat tatacttgta tagaaagaat
     5221 tttaaagtat ggcttatcat tgatttaata taataaataa tttttgtttc tagtaatagt
     5281 tcctaggaat aagatatcta aattcgttta gaaatagtct aatttacaaa ggaggggatg
     5341 cactaataga taaaatttct atataaagaa gacttaaagt gaagtatatt ctgataatat
     5401 tggtttaatg ggtatattat tatttcgttt tagatatttt aagaataatt taagacgttt
     5461 ataaaaatgt attctatatc taatatttaa ataaatataa gtcttccaac atgtatacta
     5521 atatagagag tagttgaagt acaattctaa ctcgtaaaat tatgtagaaa gtaaaaatca
     5581 aaaagaaatc tagtaagttg ataactatag ttaatattta gagagttaac tctagttata
     5641 tttatttaga tattttagga ataatttagg acgtatataa tatcatcaaa aaatatcaag
     5701 ctataccata tgtagttaag aataactgca taatatacat tttataatta ttgttttatt
     5761 gataaaaaat tattatattt aggaggaaac aacaatggaa aatttttgga atccacttac
     5821 ttgctttact gtatgtgtaa tgatttatgc ttttagtgag tttttatcta agaaaacaaa
     5881 aggtgcaatt tcaacattgt tatttgtatg tattatattt ttactaggat tttggacaaa
     5941 tattttacca aaagacatta caggagcatc agggatatta gctgcaatga gcaactttgg
     6001 tatagcttta ttaataacta acttaggaac acttataaat cttgaagact tatgtcatga
     6061 atggaaaacg gttgtaattg cattagtagg tattgtagga ataggagttg gctgtttaac
     6121 agtgggtagt atgttatttg gcagagagta tgctttaata gcagcacctc caatagcagg
     6181 ttctactgtt gctgggatta tcgtaacaac tgttgcagaa gctgcaaata gacctgaact
     6241 tgctgcattt gtaattctag tattatcatt tcaaaagttc tttggtattc cgataactac
     6301 atattgtata aataaagaat taaaatttaa aagagataac ggggattttt tgaaagatga
     6361 gtcaaaaagt aattttaaac ttccaagtat gagaattttt aaagaagatt taggtaaaaa
     6421 atcatctact ctagtgtatc ttgcaaagct ttcatttgtt gctatgattg gtaattttgt
     6481 aggattggct actttaatac caggatttac ccctgcaaac tattatttaa atcctaatat
     6541 agcatgtttg ttagtagggt tgatttttac acgaataggt tttttagaaa aaggaatttt
     6601 agataaatct caatctaatg gaattataat gtttggttgt atgttgatgt tacctggagg
     6661 acttgctaag gtaactccaa cagcattgtt tgggatgatt gttccaacta taggaatact
     6721 tgttgtaggt tctgtattta taatattatt tactgcaatt gtaggaaaac tattgggata
     6781 ttctccaaga atgtcagctg caattggtgt aacttgtatg ctagcttatc ctgcaacaca
     6841 gataatttcc aacgaaggta ttgatgctat ggaaggaact gaagaagaaa aacaaagggc
     6901 aacagattat ttattaccaa agatgattat aggaggattt gttactgtaa ccattgcatc
     6961 agtggcattt gctagtataa taggtccaat tatatttggt tgatagtaaa tatacttata
     7021 ggaggaatag gaaatgatag gaagtatatc tataaatgag atagataaaa aaagattcaa
     7081 acttgatgat ttaagtaaga aaatatggga aaatccagaa aaagcattta aagaatttaa
     7141 agcatgtgaa aatacagcaa actttctaag aagtgaagga tttgatgtgg aagtaggtgt
     7201 aggaggttta gccacggcta ttagagctag ttttggttca ggtaaaccag ttataggttt
     7261 tatgggagaa tttgatgcat tacctggttt aaatcaaaaa gtttcaacaa agcaagaagc
     7321 atttgaattg ggtgcatatg gtcatggatg tgggcataat ttattatgta cagcacatgt
     7381 aggggcagta gtagggctta aaagagagat gatagaaaat aacttaagtg ggactatagt
     7441 attttatgca tgtccaggag aagaacaatt aacaggtaaa ggatttatgg ctagaggtgg
     7501 agcgtttgaa gggttagact tagctataaa ttttcatcca aataaaatta gtgaagctac
     7561 aattggtact tcaacagctg ttaactctgt aaagtttcac tttaagggaa agactgcaca
     7621 tgcagggtca gaccctcaaa atggaagaag tgcactagat gctgtggaac ttactaatgt
     7681 gggagctaat tatttgagag aacatgttac ttctgatgta aggattcatt atacaattac
     7741 agatggagga gtagcaccca acatagttcc tgataaagca tcagtatggt attatacaag
     7801 agctttaagt agagaagctg tagaaagtac atatgaaaga cttgtaaagg tagcaaaagg
     7861 agctgcaatg atgactgaaa ctgaagttga agtagagttt ttaggagggt gttataatac
     7921 tttaaacaat catgttttag caaatcttgt atcagaatgt atgaatgaga ttaaacaaga
     7981 gccttggact cgagaggaat tggattttgc agctgaatta gatagacaaa gcccacaaca
     8041 atataaggct atgataagta aatataattt gccagaagga actcatctat attatggtgg
     8101 aggaaatgtt acaaacttta atagttatgg ctctactgat attggagatg taatgcatat
     8161 agttccgaca gcatactttt ttacagggtg tacgaatcta ggagcaccag gtcatagctg
     8221 gcagtttaca tcttgtgcag gaagttctat aggagaaaaa ggtatgatat atgcatcaaa
     8281 gattatggct atgtttggag ctaaaatatt aaataatcca gagatagcta aaaaggcaaa
     8341 agaagaattt gataaatcta tgaatggtaa aacatataaa tgtcctattc cagatgatat
     8401 tccaactcct taaaaagaca tgaaatttaa tatataaaat tataattttt atatcagcat
     8461 catttcatta ctaaaagaaa gtgagatttt gctgactatc tgattaggag gaaggaatat
     8521 gaaaaatttt tttaaagaag cgcagtcaat agaaggggaa attattagat ggagaaggta
     8581 tttacatgaa aatcccgaaa ttggttgcaa tctaccgaaa acagtaacct ttgttaccag
     8641 tactttagaa aaaatgggat attctccgaa attgacgtct aataatggaa tagtagctgt
     8701 tttagacagt gaaaatggtg gaaagacctt attattaagg gcagatatgg atgcacttcc
     8761 tattagggag gagacggatt taccttttaa aagtaagaat aaagatgcat ctcatagttg
     8821 tggacacgat aatcacacag caatgctctt aggagcagct aaaatactta tggataataa
     8881 gcacttactt aaaggtagag taaagtttgt atttgaacca gatgaagaga ctaccagagg
     8941 tgcaaaatca atgattgatg atggtatatt agaaaatcct caggtcgatg cagctatggc
     9001 atttcacagt atggtgggta aatttcttaa cacaggtcaa gtagcttatt cacgtggtcc
     9061 agctatggca tctgcggata tatttgaaat aacagtagaa ggaaaagcaa ctcatggagc
     9121 aagccctgaa tttggagtgg acccaataaa cataatgtcg catatacaaa tagcattaaa
     9181 cactatactt tcaagagaaa aacctcagaa agagcctgta gttttaacta ttggtatgtt
     9241 taatgcagga aacgtaccaa atgctatacc aaataaagca tctatgtcag gaactttaag
     9301 aacatttgac caatttgtta gagaaaaatt aaaaaataga attattgaaa tatctacagg
     9361 aatagctaaa tcatttggag gaaatgctaa agtggagttt aaaatgggga ctcctgcagt
     9421 tataaatgat ttggatgtag gagatgagat tgtatcttat gttaaagaag ttataggaaa
     9481 tgaaaatgta atagactttc catctgcaat gggttcagaa gatttttcag aggttttact
     9541 aagggttcca ggagtatttt tatggattgg aatgggaagt attagtcagg gatataattt
     9601 tggtatgcat aatcctaaag caatttttaa tgaagaggga ttagtttatg gttcagcttt
     9661 atttgctcat tgtgcagtaa aatggttaga aaataatttt agtagataat aacataaaat
     9721 attattaaat atcataaaag gtatatctta aaaagatgca aagatatacc ttttagaaag
     9781 tcatatataa ttttacttaa tattagatga taaaatattt ttatgcattt tggattttaa
     9841 tatttatttt ctgatataaa taatttgtat accctttcag gtctgccttt tgttgagttt
     9901 tgtttttcat ataatatagt agcgtttcca gactttaaga gactacttag aaatctatta
     9961 gcagttctaa ctgttacatt taaacatgaa gataattcct taggagtaac ttcatctctt
    10021 tgtagaattt cagtaacaga tataatcttt tgaatagtaa gagtagacaa tttagaactt
    10081 ttggcaattg tatttatatg tggagttact ttatttgaaa cagtaagtaa cttatcacac
    10141 agtaaaggtc ctattgtctc ataattttcg ttaactaaga aactgtaatt taaaggattt
    10201 attttagatt ccttattagc acttatggca ctagacatag cttgctttat atcattacca
    10261 attccatatc caatacatac tttaatattt gtactatttt caatagattc ttttatataa
    10321 catgcagtaa aatttttagt taatgattta atcgtttcac atttagtgaa aattttaaaa
    10381 gaattactat ttttttcaat tataaaatcg gttaatttat cttttttaat actatttaat
    10441 attttataaa cttggtctag agtagagtca tcataatttg tatctttaac tgttatatat
    10501 attacacttg gttgatttcc cttcattcta tgtaaatgaa tatcttttat taagttatta
    10561 aaagtatttt ttaataaatc tatatcaggg tatatgaaat gaaagtttac tcctgcattt
    10621 tgtaacatag gaacaatact actaaaacga gtagtagaaa aatcgatttt cccttcattc
    10681 catagattaa tatgtttttt tgcaatcttt ttttcaagtt ttgctatgtc agataaactc
    10741 attttagatg cattttcatc aatactatcc attaagtctc caaatgtgcc atttgataaa
    10801 taatatgata tatcttttgg attatctgcc cagtctgtta agtcaaagta tgctctggaa
    10861 aaatctaatg tattatattt aaagataagc tttagaaaca tatcatagta actttgtaag
    10921 tcagtaccaa aatcaactag aggtttttta atagttccag ccttttttgt aatagctttt
    10981 ttggggatgg ggccactaac tgcaaatcca tcaaacctat cttctatttc taaatataaa
    11041 tctccaacat gagaaaaatc tctatagata tatatttcaa tttctgcatt aatatttaac
    11101 ttttttatag aattatctac aaactctttt aaaaattcgg ttgttatgac tgctattttc
    11161 atcatagatt acttcctttt caattattat aattatttta tattaaatat tacattaatc
    11221 tattgttcta gttaaaatcg tatttcaatt aaattcaatt aaaataaata actctttata
    11281 aaaagcaatt atttaggtag aggaatattt gctaacttta atagtctttg ttaactttaa
    11341 ttttatttaa gttattatag tcttactaat tttataattg tagatttaac tactttatgt
    11401 tcttttattg tttatgtagt tgatttagaa gttattttaa ccatttattt taattgaagt
    11461 tgtttgactt ttaaatcctt tgtgtaatct ttgtttttct agccatgata aaagtatttc
    11521 tatttgtatc actatatata ataatatcat gacaaaatat ttattgtcta actaattata
    11581 atctatccaa aatgaaaatg ggtgtgagat attattacaa caaattgtat tagtattaaa
    11641 aagattaaaa aattaaaaaa ctaaacagtt aaataattaa acgattaagt gactgtcata
    11701 taacaattta taattttatt taacagccac ttaggtattt aagagcgata gaattgagtg
    11761 tatatcaaat tataataatt tcctagtaaa taattgaaaa ataaggtagt ccatttttat
    11821 atttgatatc tggtttacct tgaataagtg gatatagata atttagggct tcatcagtta
    11881 tataattaca ttcttcattt atccactctt taggaaaata ttttgtcttg ttagctattt
    11941 tagaaatatc agtatgacta acttcatact tataaggatt attgcatgtt ctattaatga
    12001 tagccataac tcctgtatat ccttttttag atagttctat cccatatgca ccaacttcag
    12061 ctgcttgttt taaatcagta tctgaagcta aatgcatagc acatctttgt attacaccaa
    12121 gttctaaggt ttttactctt gttgtaattg atgatgatac tataagattc tttagatagt
    12181 tgcttactcc acctaattgg gaatgaccaa atttatcatg agcagaagtt ttttgttcgc
    12241 atataaagtt gccgtattta tcccttatac cttctgatgc aactatataa gccttatttt
    12301 tttctttaaa tatttttcta acatcttcta taaacttatc ttcatagaaa tcaatttctg
    12361 gaaggtatat taaatcaaca agtggagtat cattaagtct agctagtgaa gaagaagctg
    12421 ctaaccatcc agtatctcta cccatagttt ctactataaa tataccatta tttttgtata
    12481 tagaaaaatc taagtatgtt tccattatta gtgtagaaat aagctttgca gcagaaccaa
    12541 atccaggaca gttgtcagtt cctaataaat cattatctat tgttttagga atacctataa
    12601 attttatttt acaagtagtg tttttgcagt attgagaaaa tttcatcact gtgtccatag
    12661 aatcatttcc gcctatataa aataatgtat ctattttata ttcatctagt atatttataa
    12721 ctctatcata atcataagaa tttattttat aatcagccag tttgtatcta caagaaccaa
    12781 gaccagacga aggtgtttgc tttaaattta atacattatc tttgtttaag gagtctgaat
    12841 ctattatatt tttgtttaat ataccttcaa tcccattgat accaaaatat actttatcaa
    12901 agtatttagt ttctaaattt ttttcataaa taccaactgc agaagcattg ataacagctg
    12961 tagggcctcc agattgggca ataatacagt ttctcatttt acatctccct ttcattaata
    13021 ttgttatgaa tggtactttt tatcgagtaa atttatattt atagtctgat tatatattaa
    13081 atgaactagc tatatcaatt gagttagtat agacaataaa tgccataaga atgtggtaaa
    13141 aaactaactt tttatataca attaatgtct ataatttacc acaatataaa ggtactttta
    13201 aggagtagtc aaagtgttgt gaaaaaaaat atatcagtgt aatattaatt tacaaagcaa
    13261 aataagttca cagttaacaa aagcagaata agttcataaa agaaatatat acagcaataa
    13321 aagtaatgct aaataaaagt aatactggat taaaaaatat taaaaaaata ttaaaaacta
    13381 atatttaata ctaattaaag taaaatatgg aggtatacaa atatgaaaca cataaaaata
    13441 gcagcaggat tagcacatgt aaattacggt catatatcag ccgtagttaa agaagcaatg
    13501 gatgcaggtg tagactacgt acattcagat gcagcagata tgcatgactt aaaaaatatg
    13561 caacttatgg gtggtcatca aataatagaa gcaataagac cggtgacaga taaaccaata
    13621 gaatgccata tatacacaag agattgtgat agattattta tagaaaaaat agcagcagca
    13681 ggatgtaact tgttgataat accagcagaa cattttttag gtgctcctct tgcatacata
    13741 ataaattact gtagagaatt tggtatgaaa gtaggattaa caattggatg ttacacacca
    13801 ctatgttttg ttgatgaagc tatatatgat atagatagat tgcaaatagt cgttcatggt
    13861 gttgatgaaa ctgatggaaa agataactgg ggatggagaa agagtgctgt tgatttagta
    13921 aaaagagcta gaaaaatgat agacgaaaag aatcctaagt gtgaattagc aatagatggt
    13981 ggactaagag cagacaatat ggagccatta atagaatgta atccagacgt tgtaatatta
    14041 tcttcagcaa tattcaagga taaagatggt ataacagcag gtgttaagaa ttgtagaaaa
    14101 gctatagatg aagcagctac aaagtttggt ttagaataag ataataaact atgataaaag
    14161 agatgctttc ttaatgtttt aaggaagcat taattttatg aaaaactaca ggaagctaga
    14221 ctaatttaat tgatttttta tactgaaaca atataatcat aggaggttat taaatgaaaa
    14281 aagaaatgat aaaagattat ttactgataa caatatcagc atttttaatg gcattagcag
    14341 ttaatttttt ctttgcagaa catacattag ctccaggagg gataacagga ttatcagtcg
    14401 tacttagcac attcttatca atgcctgttg agaatatatc attaggaata tctatgccac
    14461 ttttagtaat gggattatta tttttaggga aaggttttgg aataaaaaca ttatatataa
    14521 ctcttatggg cccattattc ttaaaagtaa taccacaaac tcacataact gacaatctat
    14581 tagtagcagg agtgataggt gggttattgg taggaactgc tatagggata gcaataataa
    14641 ggggctgtag tactggtgga actgatttag ttgcaatgct tattaataag gtaattaagt
    14701 ttctgaagtt gccagttata ttatttatgc tagatggatt tataatagta gcgtctggaa
    14761 taataagtaa gaattttatg atatctatat attcattgat atctttatta ctcataataa
    14821 aaacaatagg atttgtaact acaaagtttg gtaagaatac tgaagaaatt aataatataa
    14881 ataatgcttg tgctcaataa aaataataaa atttacttta aatataaaag tttaaaaaat
    14941 ataaaagctt aaataaaaag attaaatata aaaaatgaag gtgtctcaaa atgaattttt
    15001 tagttcatga ggcacttttt ctatgaaatc aaatatattt ttatcatttg gacaatgaaa
    15061 aataggttta tctctatgtt gagacaagcc tatttttgtt tatttataag ataatatttt
    15121 atttttttca gaatatagtt tttttataat tgtactaagt ctagaaatat cattcttact
    15181 taaaagagca tttatattta tcagttcagc attatgttca aagtctacag gagcagtggt
    15241 tagtattaag tccacatcat caaagttcgt aaatgaaata gaagatagag gtatagattt
    15301 aactatatta atctgattaa attcaaaact taacttttgg actaataaat tagatacacc
    15361 atcactacag tgacatatta aaatagtatt taggggagat ttgcttctat ctattgctga
    15421 tattaaatat aaagcaagat atcctatttc gtcatcattg acttcataat tcatcaactg
    15481 tttgataaca gtattagact ctttagcagc cttataagtg taaggaaatg ttctctttat
    15541 atcatttaat atgggatttg taatagtaaa cccatagttt attctgtgta gaactgattt
    15601 aaggtgatta gcaagagact ttattaatat taaatcatcc tttaagttta aatttagtat
    15661 acttccccaa tgaacagcta ttgcttcaga tagattatac tctgtaattt cttcattttt
    15721 aagaggttca agagtagtag ttttctttat tgatttaagt aatccttcta tatgaagaag
    15781 gatataacag acctcttcat caataaattc tacgtcaaaa gcttcactta ggttagcaca
    15841 taaaattttt gatttttcaa agtattcatg ttctaataat tctctaaaaa aaccttcaga
    15901 taagttaata tatcttttaa ttgaaattct tttaatacat atagctatat gtattaagaa
    15961 gtttatataa tactcatcac taaggttact tttttctact tgtaaggtgt gtagagtgat
    16021 ttgagataat ttctcaaaat catagttaaa tagctcctta aatatttttg ttgatggggt
    16081 atctttgcat tcattagatt tcgagaataa tatttcttta agttcagtat aacttttatt
    16141 aaccgtagca agttcaaaaa ttgcttttcg aatatctttc tcttttccct ctaaatatac
    16201 tccattattg gtttttctaa ctagagtcac tttaaagttt tgcaaaaaat tttgaataac
    16261 taccaagtct ttatgcacag tagctctact aacatgtagt tcattgcaaa tatcacgaat
    16321 cctaaactta gaatttttaa taaataattt acttagaata tacctttttc tatcttcagg
    16381 agagtatgca tatatacaat ttgatttatc attgatatct ctaataactt taagttttat
    16441 atcttcatta cctagaataa taacccctga accagttttt ttatctaggg ataaattaaa
    16501 ctctaaaagc cattcatcta aaataacaag gtcattacga atagttttat tactaacttt
    16561 taattcttcg gcaatagtat ctacagtaat atagtctgag ctgttgagta ataattcagc
    16621 aatatctaat aatcttttgt tcaatgcaaa ccctcctaaa tcttagttac tatttattaa
    16681 ccttggacca atctctttca aaagtattgg caccatctat tgtaagctgg ttaaaagaag
    16741 cttttttaaa taaatcatat gttacagctg cataatcaca tccacaaaga gatgcttgag
    16801 ttaagtggaa tacattttta atagaagctg caacaacttt agtattatat ccatttattg
    16861 atataaggtt acatatttct gtaaggtttt ttataccatc attcccattt tcatcatttc
    16921 tacctataaa agggaatagg taatcagcac ctgcatttat agctaatgat gcttgattta
    16981 cattgaaaat caaggtacat gcagttttta cacctttact atttaatatg tttataagtt
    17041 ctaagccaac ttcagagaaa tttattttta ttatagtgtt tttatttata tttaatatct
    17101 tatcacatga attaattaat tcgtataaat tatttccaat gacttcagca gttaatattt
    17161 ctggatttaa attagcacat gtctcaataa attcatggaa gtttactttc tctttaagat
    17221 acatagatgg attagcggtt attcccttaa atccatactt tagacacttt tgaatttcat
    17281 caagattagc actgtctatc aaatatttca taatttaatt ctcctttaag ctatttgtca
    17341 taaaaaagtg tatatactct atttttatta ttaagtcaat tatacaataa gattgattat
    17401 aaaattataa catgggtact taataaaatt tacataatga tatggtaata ataaaacatt
    17461 gattgtctaa taattaccat aagctttagt aaataagagt taacagtgaa ggtattgcct
    17521 taaaaatcct atgatatgta taatttgaat atgaaggcaa taatttaagg gaggaaaaat
    17581 atgattaact tttatcaacc atcaagaata aattttggac aaggaacttt aagtgaatta
    17641 cctagaataa taggaaaata tggaaagaaa tgtttgttag ttactactcc aaatgtagag
    17701 ccattagaca agttatataa aagagttaca ttgaatttaa tagaaaaagg tatagaagtt
    17761 gtacactttg attaaaaaat agctacgcac actcgtgact ttagtcatga gttaatagct
    17821 aaaatagtca gcatataggg aaacctatat gtagagatag gatagaacct atccaatact
    17881 acttgaattg ctggaaaccc ctaaagctaa ccaaactaaa acataaggat gaaataaacc
    17941 taagtgtgaa agttacgaaa gtagaaaaaa ttggttagat ggtataaggt taaatcctaa
    18001 gtactttaaa atgggcaatc agcaggtaag ctccgaatag gagaaacttc aacgactatt
    18061 cctcttgagg gaagtacact acaagttat
//
LOCUS       DAMJVK010000080        18012 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.081, whole genome shotgun sequence.
ACCESSION   DAMJVK010000080 DAMJVK010000000
VERSION     DAMJVK010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18012
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.081"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            30..1310
                     /locus_tag="PC414_002767"
     CDS             30..1310
                     /locus_tag="PC414_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HDF2465154.1"
                     /translation="MNILGLIVEYNPFHNGHIYHLSKSIEKTNATHTVAIMSGNFLQR
                     GEPALFDKYTRAKIAVENGVDLVIELPTLFACQSAEIFSHGAITILNSLNCINSICFG
                     SEEGNTEILYNLAEILANEPNEFKLLLKKYLDDGLLFPTARANALFDYIHQDKKNNFI
                     QNYSKEQLSKTLNSSNNILGIEYIKNLIHLNSSIKPFTISRVQSEYNSEEIESDICSA
                     TAIRTSLKENTDLFHLKNVIPEKTYNILNEKIDNGFLPMFDDFFFDILKTIILRDMDT
                     LKEYFEVNEGIENRIYKDIFTSEFLHELQLAIKSKRYTLTKIKRTLNNILLGIKKSDV
                     QLIKNIDTIPYIRILAFNDKGREILKEIKNKSEIKIINKFSNISFSLDDTIFKTLISY
                     DIKATNIYNSIYYKNNKDLVKGPMDFYTHPIYVK"
     gene            complement(1573..2964)
                     /locus_tag="PC414_002768"
     CDS             complement(1573..2964)
                     /locus_tag="PC414_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDF2465155.1"
                     /translation="MYKLIDKKDIDFLIDTCGEENVLVGSDINEDFSHDELGGIEKYP
                     EVLVNVLETEQVSKIMKYAYKNNIPVTPRGQGTGLVGAAVAINGGIMINLCKMNKILE
                     VDYENLTLTVEPGVLLMTIGQYVQDRDLFYPPDPGEKSATIAGNINTNAGGMRAVKYG
                     VTRDYVRGLEVVLPNGEIINVGGKVVKNSSGYSIKDLLVGSEGTLGIVTKAILKLLPL
                     PKKSISLLIPFPDLSMAIETVPKIIKSKSIPTAIEFMERDVILAAEEFLGKKFPDNTS
                     DAYLLLTFDGNSTEDIEKEYEKVANLCLENGALDVFISDTQERNDSIWSARGAFLEAI
                     KASTTQMDECDVVVPRDKIAEFIRYTHELQDKLKIRIKSFGHAGDGNLHIYILKDGMD
                     DNTWKIRLKETFDYMYKKSRELSGQVSGEHGIGYAKKEYLHESNSDAYMMLIKNIKLA
                     FDPKNILNPGKIY"
     gene            complement(2975..4195)
                     /locus_tag="PC414_002769"
     CDS             complement(2975..4195)
                     /locus_tag="PC414_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HDF2465156.1"
                     /translation="MSKIVVNQDKITDLKRILEICPFGAIEEKSGIVEISAGCKMCKL
                     CVKSGPKGAFEFIESSKVQINKDEWRGIAVYVEHHNGNIHPVTYELIGKAREMASKIK
                     QPVYCVFVGKDIKDKCSNLISYGVDEVFVYDEDEFKDFRIEPYSKAIENFIDKIKPTI
                     VLVGGTTLGRSLAPRLAARFRTGLTADCTILDIQSNTDLDQIRPAFGGNIMAHINTPN
                     NRPQFATVRYKIFSAPEKIENTTGKITLCKVEKKELKSKIKVLSVKEKNKEVGLEEAE
                     VIVVASRAIKKQEDMEMIYKLADKLNAQVAGTRPVIEAGWIDAKKQIGLSGRTVKPKL
                     IITCGVSGAVQFVAGMQGADYIVAINKDDKAPILDVAHLALIGDIYDIIPKLIEKIEN
                     NKDNNQKYMASAAK"
     gene            complement(4209..4997)
                     /locus_tag="PC414_002770"
     CDS             complement(4209..4997)
                     /locus_tag="PC414_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HDF2465157.1"
                     /translation="MEILTCIKQVPGTTSVEVDETTGVLKRDGVDSKMNPYDLYALET
                     ALRIKEDKKANLKVLSMGPPQAKKVIEESFMMGADEGALISDRRFGGADVLATSYTIS
                     QGIKKMGKVDLIICGKQTTDGDTAQVGPEVAEFLDIPHVTNVTKLIEVKDESIVVEID
                     MPNDLQVCEIEYPCLITVEKDIFQPRLPSFKLKLNTKDREIPVYSLDDFEDKDENNYG
                     LNGSPTQVVRIFPPKPNTDKNIVRGNADELSFALVNKLEELKLV"
     gene            complement(5014..6288)
                     /gene="larA"
                     /locus_tag="PC414_002771"
     CDS             complement(5014..6288)
                     /gene="larA"
                     /locus_tag="PC414_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HDF2465158.1"
                     /translation="MVKLKVPYSKQGMILEIPDERFLGVLESKSDNYIEHKTQEEIVE
                     ESMNNPIGSNSLEELVKGKKNVVIITSDHTRPVPSRITMPIILRRIRNVNPEIDVKII
                     VATGFHRPSTREELIYKMGEEIVDNEDIVMHISTDDKSMCKVGILPSGGDLYINKLAY
                     EAELLIAEGFIEPHFFAGFSGGRKSVLPGIASAKTIMYNHCSDFIDSDNSRTGKLNNN
                     PIHEDMVYAAKVAKLAFILNVVIDKDKKIIASFAGDVEKAHTKGCRFVTELSKVNSIK
                     SDIVVTTNGGYPLDQNIYQSVKGMTAAEATCKDGGVIIMISACNDGHGGQSFYENVAN
                     ADSPKQLLDKIRSVDRVDTIPDQWEFQILARILSKYTVIMVTDKCNPEMIKKMHMKHA
                     FNFKEALEMANSIVGKESKVVVIPDGVSVIVK"
     gene            complement(6406..7119)
                     /locus_tag="PC414_002772"
     CDS             complement(6406..7119)
                     /locus_tag="PC414_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDF2465159.1"
                     /translation="MFNSIASKKVYEQVIEQIQYKILNGELKKGDKLLSERELSEQMN
                     VSRTSIREAIRVLETMGVIESRQGEGNFICTNIEKTLIEPLSMIFKLNNGTLEDILEL
                     RIILEIEIAKLASKRITSSEVIELKHIIDEMRVETNKKDNNRVLVLLDQKFHSKLATL
                     SKNYLIQSLFMTASKLFDGFIEDAREKIIAEPFNENILLKQHEAIYNAVVENDVELAC
                     EKAKEHMDFISKNYRKNEN"
     gene            7244..8503
                     /locus_tag="PC414_002773"
     CDS             7244..8503
                     /locus_tag="PC414_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YlbJ"
                     /protein_id="HDF2465160.1"
                     /translation="MKKNKIIAFFAPSIIVGVLVLGIILYPNEAIKSAREGFSIWSNV
                     LVPSLLPFIIGANLIVDLKIVDIIGFIINPITRFVFNVSGKSALAFAISTVSGYPVGV
                     SLASEFRSNGQISKYEAQRLVSFCSTSGPLFIIGSVGTGMFQNSNLGYLMILCHYLGT
                     ILVGLFFRNYGNENLPKTKLDLKTNIKNVINIRNSSGNGFFVLFGNAVFNGVNTLLTV
                     GGFVIVFSVVFKILSLFNVISLIASVIYLPLSFLGVSKELCQAFVSGLFEITIGCNKV
                     SSITSSPEILRASLASFLIGFSGLSILAQCCTFLSKTDIDLKLYILSKFAHGVLAAIF
                     IFILYPIANSAVLVSSFADTYNVIYNNLIWFYYLSYYDTILQIVVIIYLISAVFIAKK
                     HMKTLSTNGKLMRYKNTFFTKKFSRKK"
     gene            8783..9349
                     /locus_tag="PC414_002774"
     CDS             8783..9349
                     /locus_tag="PC414_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF2465161.1"
                     /translation="MATRPIEIDEYKKIMELLHTGFTYSENGVEKRFRKNPKVALALM
                     LEANLGLRISDILRLKIGNFKGNMLEINEKKTGKLQYRPVNKNIIESINEHARKYNLK
                     SNDYLVNIKTKAIQKQLRIICKYLNLYNISTHSFRKLYATTQFEKSNNNLELVKELLN
                     HSSVATTQRYIRVTQQAINEASENFFIG"
     gene            complement(9550..10755)
                     /locus_tag="PC414_002775"
     CDS             complement(9550..10755)
                     /locus_tag="PC414_002775"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HDF2465162.1"
                     /translation="MKIAILGYGTVGSGLIDLIENNTSKRDIEVVGILVKNKEKHKYK
                     KYFDKITTDIEDIFDKDIDILVEVIGGLNPAFDYVTRALNKKIHVVTANKDLLAEKGS
                     DLIELANLNNVSIKFEASVAGGIPVLKPLIESLEGNNIKSINAILNGTCNFILSKMYD
                     ENLPYDVALKQAQELGFAEVNPDADVLGYDSARKLSILSTLSYGKIVYWKDLLLEGIT
                     SIDEKDIEYAKKLNCKIKLVARSKYESGEVSGFVRPALVDNNNMLSKIDNEFNVVILV
                     GDSVGELSFVGKGAGRGATGSAVYADIIDIIDNRSSNIKSFSKGKLDLSGLIEDECSA
                     VVRFGGYNNKDGILECLDKYVDSYDIIDDEELAIFVSAKSEHEIDRVLEIVKRNNYSE
                     SAKKLLKID"
     regulatory      complement(10858..10964)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(11056..11424)
                     /locus_tag="PC414_002776"
     CDS             complement(11056..11424)
                     /locus_tag="PC414_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HDF2465163.1"
                     /translation="MLKGKQRAYLRSLANTLKPTTQIGKEGVTESFLEQLDGMLRTRE
                     LVKVTILENAGLDTKETANAVCEALRAEFVQAIGFKFTIYKRNIEEPKILFPGHEQAK
                     AKTKNNNVTKKGKPTKRAVR"
     gene            complement(11476..12753)
                     /gene="obgE"
                     /locus_tag="PC414_002777"
     CDS             complement(11476..12753)
                     /gene="obgE"
                     /locus_tag="PC414_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HDF2465164.1"
                     /translation="MFIDKARIFVKAGNGGNGSVAFRREKYVPAGGPDGGDGGRGASI
                     IFEVDLGLRTLMDFKYQKKYQAQNGGDGSKGKRAGKNGENLVLKVPAGTVIRDEATGL
                     VLADLKKEGDTAIVAKGGIGGKGNQHFANAVRQAPAFAKSGTDGEERWITLELKMIAD
                     VGLLGFPNVGKSTFLSVVTKAKPKIANYHFTTLTPNLGVVQTKFGDSFVLADIPGIIE
                     GASEGIGLGHEFLRHVERTKVLIHIVDISGLEGRDPIEDFDKINDELKLYNEKLSKRP
                     QVVVANKFDILEDESKFEKFKSELEGRGYTVFKMSAATRQGIDEVIAYVSKMLKEVED
                     VELVSEEEMYRPELDIGTEEELSIDIEDGVYVVTGKALRRIMYSVNFDDMESLQYFQK
                     AMESQGVFDRLREMGIEDGDVVKIYELEFEFYN"
     gene            complement(12864..13154)
                     /gene="rpmA"
                     /locus_tag="PC414_002778"
     CDS             complement(12864..13154)
                     /gene="rpmA"
                     /locus_tag="PC414_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDF2465165.1"
                     /translation="MLNMNLQLLASKKGVGSSKNGRDSISKRLGVKRFDGQLVTAGSI
                     IVRQRGTKIHPGTNVGKGSDDTLFALVDGTVKFERKDKKRKKVSIYPVAIAE"
     gene            complement(13168..13485)
                     /locus_tag="PC414_002779"
     CDS             complement(13168..13485)
                     /locus_tag="PC414_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HDF2465166.1"
                     /translation="MIKVKYYYNDDFLLQGFCLKGHADFAEIGYDIVCAAVTSNAIAV
                     INSLDKLQKIEFEKVVGEEGHIECIVKDTHVKDAQLLLNHFQLAVKEIKREYPKNIKI
                     LKK"
     gene            complement(13500..13811)
                     /gene="rplU"
                     /locus_tag="PC414_002780"
     CDS             complement(13500..13811)
                     /gene="rplU"
                     /locus_tag="PC414_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDF2465167.1"
                     /translation="MYAIVKTGGKQYKVSEGDVLFVEKLEANAGDVVTLNEVLACSKD
                     GELKLGSPVVEGASVQAKVVEQGKAKKVIVFKYKAKKDYRRKQGHRQSYTKIVVEKIN
                     A"
     gene            complement(13912..15333)
                     /locus_tag="PC414_002781"
     CDS             complement(13912..15333)
                     /locus_tag="PC414_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HDF2465168.1"
                     /translation="MKKIVIESLIGSQKTAVLEDERLTELFVEDNLNKKTVSNIYRGI
                     VKKVIPGIEACFVDIGFKKLAYLQLKKGSTIKSGQDILVQINKEEIGTKGAKLNTEIS
                     ISGRYIVYIPSNDRTTISNKITDEKERFRLKKITKAVNKENLGLIIRTEAQGCTHDEI
                     KKDIEELKLKYENILKEYKLGIGPKLLYKSLDFATKYVKDNVNDDIESIITNSYDKYS
                     ELKSILRGIDKTYVDKLCLEENRDVFDLYRIESKIEKLLNKKVWLKSGGYLIIEKTEA
                     LTVIDVNTGKFIGTGKLDETVYKTNLEAAKEIVRQLRIRDIAGIIIIDFIDMHKKKHQ
                     KEILHILEEEFNKDKRKAEVLGMTKLGLVEVARRREKESIDKYYLMSCPCCDGEQTIK
                     SVHYILDSIEKEIMRISEHTVYKNIMVEFNDFIFEQIKEYYMDIIDKIGEKYNIKISL
                     NANSTLKHNKTNVIFDKIVDNKM"
     gene            complement(15351..16052)
                     /locus_tag="PC414_002782"
     CDS             complement(15351..16052)
                     /locus_tag="PC414_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2344 domain-containing protein"
                     /protein_id="HDF2465169.1"
                     /translation="MSKIIRVKFKKEGDMIYISHLDLQRLLQRAFRRAEINLSHSQGF
                     NPHPKMSYGNALALGAESQGEYVDIEIEEDDLSVEEFLNKVSIQLPDGIDFIKAKEID
                     RQTPSLSSVIDYGEYLFNIDLKRPMTKEFVKRKVIDFMNNKEIIITKKNKKGKMVEVD
                     IRPMIRTFDVLNLEDEHITLTATIATGSKTNLNTNILIPKILEMFELDIDPLDVDILR
                     RDLYVLEDGELVTPM"
     gene            complement(16054..17898)
                     /locus_tag="PC414_002783"
     CDS             complement(16054..17898)
                     /locus_tag="PC414_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HDF2465170.1"
                     /translation="MNKVDLKKILKKVEKPARYLGNEINSIHKDTSNSELIRYAHCFP
                     DLYEVGMSHLGSHILYDVINKDEDVFCERVYSPAVDMENIMREKSIPLFALESREPIT
                     NFDFVTFTLQYELSYTNILNILDLANIPILKEERTLEDPFIMVGGPCAYNSEPLADFV
                     DVVILGEGEEVNLEVVNEYKEWKKNKTTREDFLYKISSIEGVYIPSFYDVKYNEDGTV
                     QSVTPNREGITKNPTKRIIKDVETVDYPEKLIVPYIDTVHDRIVLELFRGCTRGCRFC
                     QAGMIYRPIREKSVKRLKEILDKLVKNTGYDEISLSSLSTSDYSKLSELTDYLVDEYA
                     SNNIGISLPSLRLDNFSMEIADKIQQVRKSGLTFAPEAGTQRLRDVINKGVTEEDLEN
                     ATERAFEMGWNSVKLYFMIGLPTETYEDLDGIAKLAYKVIDIYRKVNGGKLKRSFSVT
                     VSTSTFVPKPFTPFQWHGQDTTEEVINKQRHLVNKLRNNNIKYNYHDSKTSLMEAVVA
                     RGDRRIGKVIYDAFRLGAKFDGWAEHFNLDIWKEAMEKNNLSIDFYAHRNRNYEEVFP
                     WDHIDVGISKKFLIREDENAKKEKITSDCRHNCNGCGINIHDIGRGLC"
BASE COUNT         5734 a         3293 c         1923 g         7062 t
ORIGIN      
        1 aaaataatta aagataaaag gagtatttta tgaacatact tggattaata gttgaatata
       61 acccatttca taatggacac atatatcatt tatctaaatc tatagaaaaa acaaatgcaa
      121 cacatactgt agcaataatg agtggaaatt ttctacaaag aggcgaacca gctttatttg
      181 ataaatacac tagagcaaaa attgctgtcg aaaatggtgt agacttagtt atagaattac
      241 caactttatt tgcttgtcaa agtgctgaaa ttttttctca tggtgctata acaattttaa
      301 attcactaaa ctgcataaat tctatatgtt ttggtagtga agaaggaaat acagaaattc
      361 tttataatct agctgaaatt ttagcaaatg aaccaaatga atttaagtta cttttaaaaa
      421 aatatttaga tgatggtctg ttatttccaa cagctagagc taatgcactt tttgattata
      481 tacatcaaga caaaaaaaat aattttatac aaaattattc aaaggaacaa ttatcaaaaa
      541 cattaaattc atcaaacaac atactgggta tagaatacat aaaaaacctc atccatttaa
      601 acagtagtat aaaaccattt actatctcca gggtccaatc agaatataat tccgaagaaa
      661 ttgagagtga tatttgttct gctactgcta taagaacttc acttaaagaa aatacagacc
      721 tttttcattt aaaaaatgtt attccagaaa aaacttataa catcctaaat gaaaaaatag
      781 ataatggttt tttacctatg tttgatgact tcttctttga tattttaaaa acaattatac
      841 ttagagatat ggacacttta aaagagtatt ttgaagtaaa tgaaggcata gaaaacagaa
      901 tctataaaga tatctttaca tctgaatttc tacatgaact acaattagca attaaatcca
      961 agcgatacac tttaaccaaa atcaaacgaa ctctaaacaa tatattgtta ggtattaaaa
     1021 aaagcgacgt gcaacttata aaaaacatag atactatacc ttatataaga atacttgctt
     1081 ttaatgataa aggtagagaa attctaaaag agataaaaaa taaatctgaa ataaaaataa
     1141 taaacaagtt ttcaaatata tcattttcat tagatgatac gatttttaaa acactcataa
     1201 gttatgatat aaaagctaca aatatatata attcaatata ttataaaaac aataaagatt
     1261 tagtaaaagg ccctatggat ttttatactc atccaatata tgtaaagtag ttttttaaaa
     1321 taactatatt tataaaaatc attttattaa ctctttatta tataagatac aatagtctta
     1381 ctttatttat gtacacatgc ttttaggatg acaacatcaa cttaaatatt atgttacttt
     1441 ctaaatttaa gtaaatattg ccatcctaaa aattttacaa tttagcttca gatttaaaag
     1501 taaagtttaa aatattatat ttaaatctaa tttaatattt tttgttttat aaatattatt
     1561 aaattaatat ttctaatata ttttccctgg atttaaaata tttttagggt caaaagctaa
     1621 ctttatattt ttaattaaca tcatataagc atctgaattt gattcatgta agtactcttt
     1681 tttagcatat cctatgccat gttcaccaga aacttgacca ctaagttctc tagatttttt
     1741 atacatatag tcaaaagttt cttttaatct tattttccat gtattatcat ccataccatc
     1801 ttttaaaata tatatgtgta gatttccatc tcctgcatga ccaaaacttt ttattcttat
     1861 ttttaatttg tcttgaagct cgtgagtgta tcttataaac tctgctatct tatctcttgg
     1921 aacaacgaca tcacattcat ccatttgtgt cgtagatgcc tttatagctt ctaaaaatgc
     1981 acctctggct gaccatatag aatcgtttct ttcttgtgta tctgatataa atacatctaa
     2041 tgcaccattt tctaaacata aattagctac tttttcatat tctttttcta tatcttcagt
     2101 gctatttcca tcaaatgtaa gcaaaagata agcatctgat gtattatctg gaaacttttt
     2161 acctaaaaat tcttctgctg ccaatatcac atctctttcc ataaattcta tagccgttgg
     2221 tattgatttt gattttatta ttttaggaac agtttctatt gccattgata aatctggaaa
     2281 tggtataagc aaacttatac ttttctttgg taaaggaagc aattttaata tagctttagt
     2341 aactattcct aaagtacctt ctgagcccac taataaatcc tttatactat atccagaact
     2401 atttttaacc acctttccac ctacatttat aatttctcca tttggtagta caacttcaag
     2461 tcctcttaca taatcccttg taacgccata ttttactgct ctcataccac ctgcattagt
     2521 gtttatatta cctgcaattg tagcactttt ttcacctggg tcaggtgggt aaaataaatc
     2581 tctatcttga acatattgac ctattgtcat caataaaaca cctggttcta ctgtcaatgt
     2641 taaattttca taatcaactt caagtatttt attcatttta catagattaa tcattattcc
     2701 accatttatg gcaacagcag cacctacaag accagtacct tgacctcttg gtgtgactgg
     2761 tatattattt ttatatgcgt atttcattat tttagatact tgttcagttt caagtacatt
     2821 tactaaaact tcagggtact tctcaatccc tccaagttca tcatgtgaaa aatcttcatt
     2881 tatatcactt ccaactaata cattttcttc tccacaagta tctattaaaa aatctatatc
     2941 ttttttatcg attaatttat acatttctat ccccctattt agctgctgat gccatatatt
     3001 tttgattatt atctttgtta ttttctattt tttcaatcaa tttaggaata atatcataaa
     3061 tatcacctat taaagctaaa tgtgctacat caagtatagg cgctttatca tctttattta
     3121 ttgctactat atagtcagca ccctgcatcc ctgcaacaaa ttgaactgct ccagatacac
     3181 cacaagttat tatcaattta ggttttacag ttctaccact aagacctatt tgttttttag
     3241 catctatcca acctgcttct attacaggtc ttgttcctgc cacttgagcg tttaacttat
     3301 cagctaattt atatatcatc tccatatctt cttgtttttt tattgctcta cttgccacaa
     3361 ctatcacttc agcttcttcc agaccaactt ctttattttt ttcttttaca ctcagtacct
     3421 taattttaga ttttagttct tttttttcta ctttacataa agttatcttt cctgtagtat
     3481 tctctatttt ttctggtgct gaaaatattt tatatcttac agtggcaaat tgtggtctat
     3541 tattaggtgt gtttatatga gccattatat ttccaccaaa tgctggtctt atttggtcta
     3601 aatcagtatt tgattgaata tcaagtattg tacaatctgc tgtaagacct gttctaaatc
     3661 tagcggcaag tcttggagct aatgacctac ctaatgtagt tccaccaact aatactatcg
     3721 ttggttttat tttatctata aagttttcta ttgcttttga atatggttct atcctaaaat
     3781 ctttaaattc atcttcatca tatacaaata cttcatccac cccataagaa atcaaattac
     3841 tgcacttatc ttttatgtct tttcctacaa atacacaata cacaggttgt tttatttttg
     3901 atgccatctc acgagcttta cctataagct cgtacgttac aggatgtata tttccattat
     3961 gatgttcaac atatactgca attcccctcc attcatcttt attaatttgt actttactac
     4021 tctctataaa ttcaaatgcg ccttttggtc cacttttgac acacaacttg cacatcttac
     4081 agccagcact tatttctact atacctgact tttcttctat agccccaaaa ggacatattt
     4141 ctaaaattct ctttaagtca gttattttat cttgatttac aactatcttt gacatttatt
     4201 actccccctt acaccaactt taattcctct aatttattta caagtgcaaa acttagttca
     4261 tctgcatttc ctcttacaat atttttatct gtatttggct ttggaggaaa tattcttaca
     4321 acttgagtag gagaaccatt aagtccatag ttattttcat ctttatcttc aaagtcatct
     4381 aagctatata caggtatttc tctatcttta gtattaagtt ttaatttaaa tgaaggtagt
     4441 cttggttgaa aaatgtcttt ttctacagtt attaaacatg gatattcaat ctcacatact
     4501 tgtaaatcat ttggcatatc tatttcaaca actatacttt catctttaac ttcaattaat
     4561 tttgtaacgt tagtaacatg aggtatatct aaaaattcag ctacttcagg tcccacctgt
     4621 gctgtatcac catcagtagt ttgttttcca caaattatta ggtctacctt tcccatcttc
     4681 ttaatacctt gtgatatggt ataggaagta gccaatacat ctgctcctcc aaatctccta
     4741 tcacttatta atgctccttc atcagctccc atcataaaac tttcttcaat taccttcttt
     4801 gcctgaggag gacccatgct aagtactttt agattcgctt tcttatcttc ctttattcta
     4861 agagctgttt ctaaagcata taggtcatag ggattcattt ttgaatctac tccatctctt
     4921 tttagtactc ctgtagtttc atcaacttct actgaagtcg ttccaggaac ttgtttaata
     4981 catgtcaaaa tttccataaa gtacactcct tttttattta actataactg atactccatc
     5041 aggtataact actactttgc tctccttacc aactatacta ttcgccattt ctaaagcttc
     5101 tttaaagtta aatgcatgtt tcatatgcat tttttttatc atttcaggat tacacttatc
     5161 tgttaccatt atcacagtat atttagataa tattctcgct agtatctgaa attcccattg
     5221 gtctggaatc gtatctactc tatcgacact tctaatttta tctaaaagtt gttttggaga
     5281 atctgcattt gctacatttt cataaaaaga ttgacctcca tgaccatcat tacatgctga
     5341 aatcattatt attactccac cgtctttaca agtagcttca gctgctgtca ttcctttaac
     5401 agattgataa atattttggt ctagtggata tccaccattt gtagttacaa caatatcaga
     5461 ttttatagaa tttacttttg ataactctgt tacaaatcta catcctttag tatgtgcttt
     5521 ttcgacatca ccagcaaagg aagcaattat ttttttatct ttatctatta caacattcaa
     5581 tatgaatgct aattttgcaa ctttagctgc atataccata tcctcatgaa taggattatt
     5641 attcaatttt ccagttcttg aattatcact atctataaag tcactacaat gattgtacat
     5701 tatagtcttt gcagatgcga ttcctggaag tacacttttt ctgccacctg aaaaacctgc
     5761 aaagaaatga ggttctataa aaccttctgc tatcaaaagt tctgcctcat atgctagttt
     5821 gtttatatat aaatctccac ctgaaggcaa tatccctact ttacacatag atttatcatc
     5881 agtagatata tgcataacta tatcttcatt atctactatt tcttctccca ttttataaat
     5941 tagctcttcc cttgtacttg gcctatgaaa tccagttgcc actataatct tcacatcaat
     6001 ttctggatta acattcctaa tccttcttaa tataattggc atagttattc tacttggtac
     6061 aggtcttgta tggtcacttg taattatgac tacattcttt ttccccttaa ccagttcttc
     6121 cagagaatta gagcctatag gattattcat agattcttca actatctctt cttgagtctt
     6181 atgttctata taattatccg attttgactc caaaacaccg agaaaacgtt catcaggtat
     6241 ttctaaaatc attccttgtt ttgaatacgg tacttttaac tttaccattt ttaatctccc
     6301 attataataa actataaaat tggtatgtgg tcataccagt tttacattta tatgatagta
     6361 ccattatcta ccatttgcaa gaataattta ttttataaca aacttttaat tttcattttt
     6421 tctataattt ttacttataa aatccatatg ttcctttgct ttctcacatg caagttctac
     6481 atcattttct acaactgcat tgtatatagc ttcatgctgt ttaagcaata tattttcatt
     6541 aaaaggttct gcgattattt tttctcttgc atcttctata aatccatcaa ataattttga
     6601 tgctgtcata aaaagacttt gaattaaata atttttactt aatgttgcta gtttactgtg
     6661 aaatttttgg tctaataaca ccagtactcg attattatct tttttattag tttctaccct
     6721 catttcatct attatatgtt tcaattctat aacttcagaa gaagttattc tctttgaagc
     6781 aagttttgca atctcaattt ctaggattat tctaagctct aatatatctt caagtgtacc
     6841 attattaagt ttaaaaatca tagataaagg ttctattaaa gttttttcta tatttgtaca
     6901 tataaaattt ccttcaccct gtctgctttc tatcactccc atagtctcta ataccctaat
     6961 agcttccctt atggaagttc tactcacatt catttgttca gacagttctc tttctgatag
     7021 taatttatcc ccctttttta actctccatt tagtatttta tattgtattt gctcaattac
     7081 ttgttcataa acttttttac tagctatact attaaacatt ttttcctcca atatgataat
     7141 cgttgttgta atatatatct attatatatc ttaagccatt acattcatat atttttatta
     7201 cttttaatat aatatcttat aactcaaatt tacaggtgat attatgaaaa aaaataaaat
     7261 tattgctttt tttgctccat ctatcatagt tggagttcta gttttaggga ttatcctata
     7321 cccaaatgaa gctataaaat ctgcaagaga aggcttttct atatggtcta atgttttagt
     7381 tccatcacta ctaccattta taattggagc aaacttaatt gttgatttaa aaatagtgga
     7441 tattataggg tttatcatca atcccataac tagatttgta tttaatgttt caggaaagag
     7501 cgctttagct tttgctatat ctacagtatc tggttatcca gtaggagtaa gtttagcttc
     7561 tgaatttagg agtaatggac aaatttctaa gtatgaagca caaagacttg tgtctttttg
     7621 ttctacatca ggacctttat tcataatagg ttcggttggt acaggtatgt tccaaaattc
     7681 aaacttaggt tatttaatga ttctctgcca ttatctagga actatattgg ttggtctttt
     7741 ctttagaaat tatggtaatg aaaatcttcc aaagacaaaa ttggatttaa aaacaaatat
     7801 aaaaaatgta atcaacatta gaaattcaag tgggaatggt ttttttgttt tatttggaaa
     7861 tgctgtattt aatggagtaa atacattact tacagttggt ggatttgtaa ttgttttttc
     7921 agtagtattt aaaatactat ctctttttaa tgtaatctca ttaatagcat cagttatata
     7981 tttgcctctt tcatttttag gtgttagtaa agaactctgt caagcatttg taagtggtct
     8041 atttgaaatt actataggat gcaataaagt atctagtata acaagttctc cagaaatttt
     8101 aagagcatct ttagctagtt ttttgatagg atttagtgga ttatccatcc tagctcaatg
     8161 ttgtacattt ctatcaaaaa cggacataga tttaaaatta tacatactaa gtaaatttgc
     8221 gcatggagtt ttagctgcta tctttatatt tatactttat ccaattgcaa actctgctgt
     8281 gttggtttct agctttgctg atacatacaa cgttatatat aataatttaa tctggtttta
     8341 ttatcttagc tattatgata caattcttca aattgtagta ataatttatc taatctcagc
     8401 cgtatttatt gcaaaaaaac atatgaaaac tttatctaca aatgggaaac taatgcgata
     8461 taaaaatact tttttcacta agaaattttc acgaaaaaaa taaaactaaa aataaaacta
     8521 ataaaactta actaaatact tccatttttt tattatattt gtcataattt taataacctt
     8581 tttaattagt ttaacagcat agttatttca tcacaattta ataattttat tagaaatatt
     8641 tttcaaaaaa ttctccttaa tcacatatat cacacttgta taagtttact gggaaatttt
     8701 tccattatat aatatttcat ttatattata taataaatac cgacctaaac ttaccaaatc
     8761 aaactaagag gagtggttga aaatggcaac aagacctata gaaatagacg aatataaaaa
     8821 gataatggaa ctattacata ctggctttac ttatagtgaa aatggcgttg aaaaaagatt
     8881 tagaaaaaat cctaaagttg cattggcact aatgttagaa gctaatttag gtctaagaat
     8941 atcagatatt ttaagattaa agataggtaa ttttaaaggt aatatgttgg aaataaatga
     9001 aaaaaagacg ggcaaattac agtatagacc agttaataaa aatataattg agtccataaa
     9061 tgaacatgca agaaagtata atcttaaatc aaatgactac ttagtaaata taaagacaaa
     9121 ggctattcaa aaacagttga ggataatttg taaatactta aatctgtata atataagtac
     9181 acatagcttt agaaagctct atgctactac tcaatttgaa aagagtaaca ataatttaga
     9241 gttggttaaa gaacttttaa atcacagctc tgtagccact acacagcgat atataagggt
     9301 aactcagcaa gctattaatg aagctagtga aaatttcttt attggttaat tataacgtat
     9361 acatttttct attatcttat taattcacca gatattaaaa aagagtgtct caaaatgaac
     9421 tttttagttc atgagacact ctttttttat aaaatcaaat atatttttat tatttcaaca
     9481 ctggaaaaaa ggcttatctc tctattttga gacaccctat taaattttca taattcttgc
     9541 ataaacatat taatctatct tcaataattt tttagctgat tcactgtaat tatttctttt
     9601 tacaatttcc aaaactctat caatctcatg ttctgacttt gcacttacaa aaatcgccaa
     9661 ttcttcatca tctataatat cataactatc tacatattta tctaaacatt ctaaaattcc
     9721 atctttatta ttatacccac caaatctaac tactgcacta cactcatctt ctataagtcc
     9781 acttaaatcc aatttccctt ttgaaaaaga ctttatattt gaacttctat tatctattat
     9841 atctataata tctgcataaa cagcacttcc tgttgcacct cttccagctc ccttacctac
     9901 aaaagaaagt tcacctactg aatcacctac taaaataact acgttgaact cattatctat
     9961 ctttgaaagc atattattat tgtccactaa tgctggtcta acaaatccac ttacttctcc
    10021 tgattcatac ttacttctag caaccaattt tatcttacaa tttaattttt ttgcatattc
    10081 tatatctttt tcatctattg aagttattcc ttctaataat aaatctttcc aatatactat
    10141 ttttccataa gataaagtag acaatataga taactttcta gcactatcat atcctaaaac
    10201 atctgcatct ggattaactt ctgcaaatcc caactcttgt gcctgtttta aagcgacatc
    10261 atacggtaaa ttttcatcat acatctttga taatataaaa ttacaagtcc catttaaaat
    10321 agcgtttatg ctctttatat tgttgccttc cagtgattct atcaatggtt ttaaaactgg
    10381 aataccacct gccacagatg cttcaaactt gatgctaaca ttatttaagt tagctaattc
    10441 tataaggtca ctaccttttt cagccagtaa atctttatta gctgtcacta catgtatttt
    10501 tttatttaac gctcttgtta cataatcaaa agctggattt aatcctccaa ttacttcaac
    10561 tagtatatca atatccttgt caaatatatc ttctatatca gtagttatct tatcaaaata
    10621 cttcttatac ttgtgttttt ctttattttt aactaaaatt ccaacaactt ctatatctct
    10681 ttttgaagta ttattttcta ttaaatcaat taatccactt cctactgttc cataccctaa
    10741 tattgctatc ttcactgtaa cataccccca tcattttgat tatattaaat tatttatata
    10801 tataaatata ccaataaaaa aagcttgagt ataaactcaa gccatatgac gggtttatac
    10861 tcatctttca gcataaatgc tgcaggattt agcaccaatg tataattaaa tatactggtt
    10921 gccgggcttc acagggccag tccctctgcc actcttgata agattgctaa tttaaattta
    10981 tgtttaatag tataatacaa tttcttccaa ttgtcactat ttttttaact tatttcccta
    11041 aaaggatata aactattatc taacagctct tttagttggt ttaccctttt ttgtaacatt
    11101 attattttta gtctttgctt tcgcctgttc atgtcctgga aaaagtattt taggctcttc
    11161 aatattcctc ttgtaaatag taaatttaaa accaatagcc tgcacaaatt cagctcttaa
    11221 agcttcacat actgcattag cagtttcttt agtatcaaga cctgcatttt ctaatatagt
    11281 aacttttact aattctctag ttcttaacat accatctaat tgctctaaga aactctctgt
    11341 aacaccttct ttaccaattt gtgtagtagg ctttaaagta tttgccaaag atcttaaata
    11401 cgccctttgt tttcctttta acatattctc acctctataa ttctatactt aaattatcta
    11461 taatctatat ttaaattaat tgtaaaactc aaattctaat tcataaattt taaccacatc
    11521 tccatcttct attcccattt ctctaagcct atcaaaaaca ccttgactct ccatagcttt
    11581 ttggaaatat tgaagggatt ccatatcatc aaaatttaca gaatacatta ttcttctaag
    11641 tgctttacct gtaactacat acacaccatc ttcaatatct atacttaact cttcttcagt
    11701 accaatatca agttctggtc tatacatttc ttcttctgaa actagctcca catcttctac
    11761 ttcttttagc attttagaaa cataggctat tacttcatct atgccttgac gagttgctgc
    11821 tgacatttta aatactgtat atcctctgcc ttctaattca ctcttaaact tctcaaactt
    11881 actctcatct tctaatatat caaatttatt tgcaactaca acttgtggtc tctttgaaag
    11941 cttttcattg tacaatttaa gctcatcatt tatcttgtca aaatcttcaa tagggtctct
    12001 tccctctaat ccagatatat ctacaatatg tatcaatacc tttgttctct caacatgtct
    12061 taagaactca tgacctaatc ctatgccttc agaagcaccc tctataattc caggtatatc
    12121 tgcaagtaca aaactatctc caaacttagt ttgaacaaca cctagatttg gtgtaagtgt
    12181 tgtaaaatga tagtttgcaa ttttgggttt agctttagtt acaactgata gaaatgttga
    12241 tttacctaca tttggaaatc ctaaaagacc aacatctgct atcattttaa gttcaagagt
    12301 tatccatctt tcttctccat ctgtaccaga ttttgcaaaa gctggagctt gtcttactgc
    12361 atttgcaaag tgttggttac ctttaccacc tataccacct tttgcaacta tagctgtatc
    12421 tccttctttt tttaaatctg ctaatacaag acctgtagcc tcatctctta ttactgtacc
    12481 tgctggtact tttaatacta aattttctcc atttttacca gctctttttc ctttgcttcc
    12541 gtcgccacca ttttgagctt gatatttttt ctggtactta aagtccatta gagttctaag
    12601 ccccaaatca acttcaaata tgatgcttgc tccacgacca ccatctccac cgtctggacc
    12661 accagctgga acgtattttt ctcttctaaa tgctaccgaa ccatttccac catttccagc
    12721 cttaacaaat attcttgcct tatctataaa caaattcttc acctacttaa ttaaaaactt
    12781 ttaattagca ttaccaaaaa taccattata aattcatcag tattataaga aaaaggagtg
    12841 cctcaacact ccttttatgt atattactca gctattgcta ctggatatat actaactttt
    12901 tttctcttct tgtcttttct ttcgaattta acagtaccat caactaatgc aaataaagta
    12961 tcatcactac cttttccaac attagttcct ggatgtattt tagttcctct ttgtctaact
    13021 attatacttc cagcagttac taattgacca tcgaatctct taacacctaa tcttttagat
    13081 atagaatctc taccattttt agaagaacct accccttttt tagatgctaa taattgtaaa
    13141 ttcatgttta acatatggag tcacccccta cttttttaaa atttttatat tttttgggta
    13201 ttcccgttta atttctttaa ccgctaattg aaagtgattt agtaataatt gtgcgtcttt
    13261 tacatgtgta tctttgacta tacactctat atgaccttct tctcctacta ctttttcaaa
    13321 ttcaatcttc tgtaatttgt ctaaagaatt tataactgca atagcattag atgtaactgc
    13381 agcacatact atatcatagc ctatttcagc aaaatcagca tgtcccttta aacaaaaacc
    13441 ttgcaacaag aaatcatcat tataataata tttaactttt atcataactt aaccaataac
    13501 taagcgttta ttttttctac aactatctta gtgtatgatt gacgatgtcc ttgttttctt
    13561 ctgtaatctt tttttgcttt atacttgaaa actataactt tcttagcttt accttgttca
    13621 acaactttag cttgaacaga tgctccttct actactggag aacctaattt taattctcca
    13681 tctttagagc aagctaatac ttcgtttaaa gtaacaacat cacctgcgtt agcttctaat
    13741 ttttcaacga ataatacatc accttcagaa actttatatt gctttccacc agtctttact
    13801 atagcgtaca ttaaatacac ctcctcggcc tcaaactcgc cagtacaagg tacttcattt
    13861 gaagatttaa acctttactg tgcggcatta caattattaa ttataaaata tttacatttt
    13921 attgtcaact attttatcga aaataacatt ggttttattg tgctttaatg tactatttgc
    13981 gtttaaagaa atctttatat tatatttttc accaatttta tcaattatat ccatataata
    14041 ttcttttatt tgttcaaaaa taaaatcatt aaattctacc attatgtttt tgtaaacagt
    14101 atgttcactt attctcataa tttctttttc aatactatct agtatataat gtacagattt
    14161 tattgtttgt tcaccatcac aacatggaca agacattaaa taatacttat caatggattc
    14221 tttttctcta cgcctagcaa cttctactaa accaagtttt gtcataccta atacttcagc
    14281 ttttctttta tctttattaa attcttcttc taggatgtgt aaaatctcct tttgatgctt
    14341 ctttttatgc atatcaataa aatcgattat tattatccca gctatgtctc taattctaag
    14401 ctgtcttaca atttcttttg cagcttcaag attagtttta taaacggttt catcaagttt
    14461 accagttcca ataaacttac ccgtatttac atctataacc gtaagagctt ctgttttttc
    14521 aattattaaa taacctccac tctttaacca tactttttta tttaataatt tttctatttt
    14581 agattctatt ctgtataaat caaatacatc tctattttcc tcaagacaca gtttatcaac
    14641 ataagtctta tctatacctc ttaaaataga cttaagttca gagtacttat cataactatt
    14701 ggtaattata gattcaatat catcattcac attatctttt acatatttag ttgcaaaatc
    14761 taaagactta tataataatt tcggacctat accaagttta tattctttta aaatgttttc
    14821 atactttaat ttcaactctt ctatatcttt ctttatttca tcatgagtgc aaccttgagc
    14881 ctcagtcctt attattaatc ctagattttc cttatttact gccttagtaa ttttttttaa
    14941 tctaaatcgt tctttttcat cagtaatttt atttgatatt gtagttctat cattggaagg
    15001 tatatagaca atataccttc cagaaatact aatctcagtg tttaattttg cgcctttagt
    15061 tcctatttct tctttattta tctgaaccaa aatatcctga ccagatttta tagtacttcc
    15121 cttttttaat tgtaaataag caagtttctt aaatccaata tctacaaaac aagcttctat
    15181 tcctggtata acttttttta cgattccacg atatatattg gatacagtct tcttatttaa
    15241 attgtcttca acaaaaagtt ctgttaatct ttcatcttca agtactgctg ttttctggga
    15301 ccctattagg gactcaataa ctatcttttt cacaacttca cctctcgagc ttacatagga
    15361 gtcactaatt ccccatcttc taatacatat aaatctcttc tcaatatatc aacatctaat
    15421 gggtcaatat caagttcaaa catctctaat atctttggta taagtatatt agtatttaaa
    15481 ttagtttttg agcctgtagc tatagtagca gtaagagtaa tatgttcatc ttctaaattt
    15541 aaaacatcaa aggttcttat cataggtctt atatctactt caaccatctt acctttttta
    15601 ttcttttttg ttatgattat ttctttatta ttcataaagt caattacttt tctttttaca
    15661 aactctttcg tcataggtct tttcaaatct atattaaaca aatattctcc atagtctatc
    15721 acagaagaaa gagaaggtgt ttgtctatca atttcttttg ctttgataaa atctatccca
    15781 tctggaagtt gaattgatac tttatttaaa aattcctcta ctgataaatc atcttcttct
    15841 atttcaatat ctacatactc accttgactt tctgccccta aagccaaagc attgccataa
    15901 ctcattttag ggtgaggatt aaatccttga gaatgtgata agtttatttc agctcttcta
    15961 aacgctcttt gtaacaatct ctgaaggtca agatgtgata tatatatcat gtcgccttcc
    16021 tttttaaatt ttactcttat tatcttactc atattagcaa agcccccttc ctatatcatg
    16081 tatatttatt ccacaaccat tgcagttatg tctacaatca gaagtgattt tttctttttt
    16141 agcattttcg tcttctctaa ttaagaattt tttactaata cctacatcta tatggtccca
    16201 aggaaatacc tcttcatagt ttctatttct atgtgcataa aaatctatag aaagattatt
    16261 cttttccata gcttctttcc atatatctaa attaaaatgt tctgcccatc catcaaattt
    16321 agcacctagt ctaaatgcat catatataac ttttcctatt cttctatcgc ctctagctac
    16381 tactgcttcc atcaaactag tctttgaatc atgataattg tattttatat tgttatttct
    16441 taatttattt actaaatgtc tctgcttatt aataacttct tctgtagtat cttgaccatg
    16501 ccattggaaa ggtgtaaatg gctttggtac aaatgtagaa gtactcactg taacactaaa
    16561 acttctcttt agctttcctc catttacttt tctataaata tcaataactt tataagccaa
    16621 tttagctata ccatctaaat cttcataagt ctcagttggt aaaccaatca taaaatacaa
    16681 cttaacacta ttccacccca tctcaaaagc tctttctgtt gcattttcca aatcttcttc
    16741 tgtaacacct ttatttataa catctctaag tctttgagtt cctgcttctg gagcaaaagt
    16801 aagtccagat tttcttactt gttgtatttt atcagcaatt tccattgaaa aattatctaa
    16861 tctaagggat ggaagcgaaa tcccaatatt attagatgca tattcatcta ccaaataatc
    16921 agtcaactct gataacttac tataatcact cgtactaagt gatgataatg atatttcatc
    16981 atatccagta ttcttaacta atttatctaa tatttcttta agtcttttaa cactcttttc
    17041 tcttattggc ctgtatatca tacctgcttg acagaatcta catcctctag tacatcctct
    17101 aaataattct agtactattc tatcatgaac agtatctata taaggtacta ttagtttttc
    17161 tggataatct acagtctcaa catcttttat tatcctttta gtaggatttt tagtaatccc
    17221 ttctctatta ggagttacac tttgaactgt accatcttca ttgtacttga catcataaaa
    17281 acttggtatg tatactcctt ctatgctaga tattttatat aaaaaatctt ctctagtcgt
    17341 tttatttttc ttccattctt tatactcatt tactacttct aaattaacct cttcaccttc
    17401 acctaatatt actacatcta caaaatctgc taatggttcg gaattataag cacaaggacc
    17461 accaaccatt ataaatgggt cttccaaagt tctttcttct tttaatattg gtatattggc
    17521 taaatctaat atatttaaga tattagtgta tgaaagttca tattgaagtg taaaagtgac
    17581 aaaatcaaag tttgttatag gctctcttga ctctaatgca aataatggta tacttttttc
    17641 tctcattata ttttccatat caactgcagg agaatacact ctctcacaaa atacatcttc
    17701 atccttattt attacatcat ataatatatg actccctaag tgactcatac caacttcata
    17761 taaatctgga aagcaatgtg catatcttat caattcacta ttactagtat ctttatgaat
    17821 tgaatttatt tcatttccaa gatatctagc aggcttttct acttttttta agattttttt
    17881 caagtctact ttattcattt attaaaattc ctccaaacaa gctctttatt tttattctta
    17941 tttataagta atattataca cgataaaata ttttctatca aaaatataac taaaatattg
    18001 aatttaaaat ac
//
LOCUS       DAMJVK010000081        17973 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.082, whole genome shotgun sequence.
ACCESSION   DAMJVK010000081 DAMJVK010000000
VERSION     DAMJVK010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17973
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.082"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(127..1557)
                     /gene="dcm"
                     /locus_tag="PC414_002784"
     CDS             complement(127..1557)
                     /gene="dcm"
                     /locus_tag="PC414_002784"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF024835.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007157505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HDF2465171.1"
                     /translation="MKKITIDKNRVRKIMKEKNIRSQNQLALNMGITKNQLSAILSEK
                     FTPIKSNVEKLCEVLDIGFDEIMCLEDVNEKQLKFTNICSSNKDYIEHRDCTYKELSE
                     YELNPRERNKNNKHIDISNVLAKRAYTCVELFAGAGGLALGLEKAGFKEVGLVEWDKY
                     ACDTLKLNRPDWNVIQGDIVKIAENGIKNYIDNNIEIDLLSGGYPCQAFSYAGKKLGL
                     EDVRGTLFYSYAKILEELKPKVFLAENVRGLVSHDGGKTLKTMIDVFSEIGYNVKYEI
                     LKAVNYGVAQKRERVIIIGTRKDLSHVDFKFPKSFEYVATLRDALKNVPKSEGARYPE
                     RKKKVLDMVPPGGCWIDLPDEVARDYMGKSYYSGGGKRGMARRISWDEPCLTLTCSPA
                     QKQTERCHPDETRPFTTREYARIQSFPDEWEFTGSVSQVYKQIGNAVPVKLGKAIGLS
                     IVDYLNKIEKIYVTEEVAITEEEYVI"
     gene            1770..2486
                     /locus_tag="PC414_002785"
     CDS             1770..2486
                     /locus_tag="PC414_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002601887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HDF2465172.1"
                     /translation="MTELEKQEILDKAKDFFTNILVANHKKNTIKLNLKAFNINPFLV
                     KYLASFLTGSNKPEDIAKALIYPRVLGTSINTSFGTNLQKFCITTLDGFASTTSGIDI
                     EFIDCIDGRRKFCQIKAGPNTINKDDVKTIIDHFSSVKRLARTNGLSLSYNDLIVGVF
                     YGTENELNAHYKRINEEYPVVVGQDFWYRLTGDENFYFDLIDTIAQSALEEDCTVLLK
                     KTISNLADEISKDKSLFNQK"
     gene            complement(2731..3009)
                     /locus_tag="PC414_002786"
     CDS             complement(2731..3009)
                     /locus_tag="PC414_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465173.1"
                     /translation="MYDEIIEIYFDYWINEHMYRKVLEKDEYYNDRCKEIKKVNKLLE
                     KNLSEEKKMDVLIELNDLFNSVIEIERELGYKLGFKAGARFMIELLNS"
     gene            complement(3257..4591)
                     /locus_tag="PC414_002787"
     CDS             complement(3257..4591)
                     /locus_tag="PC414_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2465174.1"
                     /translation="MDNMSNLVKNTEAEQSILGSILLDSRKIEEIEILISEDMFYHQE
                     HKHIFNSVKDIHKRDEPIDIITLTEDLKKKGLIDYVGGISYLTKLSTIVPTIGNVSHY
                     IEIVIEKYKNRNIIDKFNKFKLGEIDDNTLIYQLEKYVNEVNQKLVKKDSSITAISER
                     AFEYINDEIEEGIDIGIKFLDETIGGLYSGELITIAAKSGRGKTALALQIMRNVVFQG
                     KKVLFISGEMSDIQILFRNIASLTGISVVKMKNRNLNEEEIIKYINALSTLNQENNLY
                     INDSVNTISGIKREIKSIKPDVVIIDYLQILDYEGKEVGEAKFADLSRQIKKITLDFN
                     IPIIQLAQLNDELKDERPKGDRVMRSSKQIYMDSNSVIYIHRPTEKEALDYCKTMGIE
                     DIKELRRREKGFGANLSEIIVAKNRDGESGFRPYWYIGSLFSFKECKKVKEV"
     gene            complement(4604..5347)
                     /locus_tag="PC414_002788"
     CDS             complement(4604..5347)
                     /locus_tag="PC414_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465175.1"
                     /translation="MSNLGWIKLHRKIKESPLYKDCNSKQRDILINLLLSVNHKPNKW
                     VYGGKEYEVKSGQLITSLESIANMCSKDVSIQNVRTALKKFERYGFLTNKSTNKNRLI
                     TIENWELYQSNEENQQTNQHAINKQLTTNKNVNNEKSKIYSEIIEKFNITCTELSKVK
                     KLTEARKKVINARIKEYDKETVLEIIHIVSKTSFLNGSNEKGWKANFDWIMSPNNFIK
                     ILEGNYQDKSINVKVDAEAYSPDDSQLKW"
     gene            complement(5368..5682)
                     /locus_tag="PC414_002789"
     CDS             complement(5368..5682)
                     /locus_tag="PC414_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465176.1"
                     /translation="MADTNNLEVLKTLQATLNILVKQEQEAQEKEKGYKEIQTYRRDK
                     AQLITCKEASKIYPIGESKFRQLCHSKNKGFPCIWIDNRVYIIKNKLDNWFIENANGI
                     KL"
     gene            complement(5686..5880)
                     /locus_tag="PC414_002790"
     CDS             complement(5686..5880)
                     /locus_tag="PC414_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2465177.1"
                     /translation="MKLKLKRIEKGIKQYELAEKVGISRYYLSSLERGKSKNPSNMLM
                     LKLAQELDTSVEELFFDKEE"
     gene            6164..6880
                     /locus_tag="PC414_002791"
     CDS             6164..6880
                     /locus_tag="PC414_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2465178.1"
                     /translation="MIKKISTALSIPINRLIIDESDLDTSTNNYTNLKELDLVVKTFD
                     NYSAKLIGNNISKFRNINNISLKELSEKINCSENEIINYENGTKMPRIEILKKIALAT
                     YTKIEDLFGFPLEEIKIDNQTILYRNPQTGYIIDNFLIDFINKDINIDNTPVSYYIDK
                     SEFDLVIHILKNYGYDVKTDYKTKFEIADNADTIKPISLSFSDFKDFSKNLNWVINGL
                     VENFIQENSNGLIEDEDEEY"
     gene            6977..7729
                     /locus_tag="PC414_002792"
     CDS             6977..7729
                     /locus_tag="PC414_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDF2465179.1"
                     /translation="MNKSYIPKYTHKFPTNLPHGDKKYTLTYIRRMISEFVYDMGNLE
                     NNPFTFPEVQTLLDGITVGGHKLSDQNQILNIKSSWDYVIKLSNKENLSLNKDTICSI
                     HKIVAKDEALIVGDFRNGNIGIAGTTQYECIEATLLNDLFVSDINIINKITNPLEKAI
                     IINLWLSYCQFFYDGNKRTARLTSNLILISNDIGVLSIPAKHKQEYNTLMLNFYETLE
                     ADEVIKFLLEKCITFFDEYNYKSYKELFKNGN"
     gene            7719..8885
                     /locus_tag="PC414_002793"
     CDS             7719..8885
                     /locus_tag="PC414_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDF2465180.1"
                     /translation="MATKRANGEGSIGKYKDGWRSRIMIGYNEDGKPIRKEFYGKTQK
                     EVKDKLDAYKKQYYLSSDISDDKITLEHWFYNWLFEYRIKDLKPKSFERYESIYRNYI
                     KDTDLGNVKLKDLRVSHIQKYYNRLLDSNKSIPTIRQINTKLKTCLSEAEKQGLIQRN
                     YCTMVNIPVEKKENKLEILTLEQQKSFIKAIDGHKLEVLFLLALGTGLRLGELLGLKW
                     FDIDFKKSNLTVKRTLQRTYFIDKTGNRELKVLEQEPKTSNSYRTVPIPKDVLNKLKE
                     HKVNQNSDILKAGELYRNDNYVFCNELGAPIDDKRPLRNLKSILNSLDIEPIKFHGLR
                     KTYATRLFENDVPPKTVQVLMGHYDISITLNIYTQVMEDKKIEAVEKLDKIFSL"
     gene            complement(9002..9232)
                     /locus_tag="PC414_002794"
     CDS             complement(9002..9232)
                     /locus_tag="PC414_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465181.1"
                     /translation="MEANKTLLQSLYKKIILEFSKQTGKSLEESMDYLYTSETYKLIS
                     EGISDMHCKGHIYLAQELMLENGLMYHKGYPR"
     gene            complement(9481..14052)
                     /locus_tag="PC414_002795"
     CDS             complement(9481..14052)
                     /locus_tag="PC414_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007062489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HDF2465182.1"
                     /translation="MTFEEIMEFNKPYNQKNFDELVNAIKDGYVVPYIGAGMSMLFKD
                     IYPSWSTFLNNTSEEYLEDEDKILFEKIEDYEKKAEFLYEELAKVRFSKHLKKVFGAG
                     YLDAKDKDDFIDKCVYLIPIIFGSGLIITTNYDKVLEKNYGLHNKTVLVSHPGHFEAL
                     NNALRNNELVLYKIHGDITEPTTSIVLTKEQYEKAYSNDELRKMLTQVYTSKELLFLG
                     CSLEKDRPLELLQQVSEAGTGHYAIIPVSSEKKKHRRIELENEYYIQTIMYPEGKHEC
                     VRIILERILEIVLPDEYRRLKDRSLKESRLTNLSCRLTEKWFQNQNNIQIKNLGDRYL
                     PELNVELSLENTFNALGKNEEFKQRFDEKTDKVLSSIKKQKINEVEVDVLVIEEIIQQ
                     FYDKANERLDIKLLKKHFEKISAALDDRINNFKNIIINEDCEKSKKREDEIYKLKQTQ
                     KDIYVYIKYLDSKEIKVVNSPYILLHSAGGMGKSHIIAHTIEKRNMDGHKSILFLGQH
                     FKLDKTPIKHMLEILEIECEFEELLEILNEIGEKEQQRVILFIDALNEGQGKELWKYY
                     LNGIVEQIKKYPWIGLILSIRSEYIPSLIDNNNKIKENLVFIEHQGFSSIEHKAMKKY
                     FDYYGIPYSNIPMPNQEFRNPLFLRIMCETFKGEEINLDTISFTDIYKNYLNILNLRI
                     SESCNYSRHANVVTEVLNEMIKYKYGDGLGNNLIPANKSVEIIINIASKYKINNVIID
                     ELIANGIVNKTVNLNDEEYMHVTYEKLEDYLYANLLVKELKEIGVVDFSHKYKYLVEY
                     EDILEVLAIVLSEETGSDIPNEIFEVFKNKETNYNVISAFINALKWRKKSTITEKTLD
                     YINKIVLGKSYYKIDFYNVLILISTKVNHTLNADYIVKYILQNTMADRDAIFIPIFNE
                     VYEEEESSIKRLLGWCFDFEGFDNTSNETIRLSAMMIATFLISSNRDLRDKSTKALVN
                     LLTGKMDIVIDLLKTFEDVDDPYIIERLYAVAFGCVVSEQSEEMIEALAVYVYNKIFD
                     VDYVYPNILLRDYAKNIVDYAKYKIDSSVLKDMNVEPPYKSIFPKVPTDEEIEGYKYD
                     YKASDFKDYYWSQNTILNSMEVEYTRDGQPGWYGDFGRYMFQHYFHKWKGLDYNDLKN
                     IAIKKVFDMGYDVNKHGKFDRTVQNGRLRDSKYERIGKKYQWIALYELAAQAADNYKI
                     EVYTDAYGTKKEIYCKGTFEPDIRNIDPTVMTVINNNNNKRVHNQLFQFSPIPNNEWL
                     GDFSDLPSINDMVNSVYKNQDFLLLNGWYTWKEENDFKNKNYKNPEKDMWVLINSYII
                     KNDQLEPIVNALKGKDFMGRWLTEPNSNYTLYNKEYYWADGYYFFQNSYYCGEEWVQI
                     DEHKNILEKDYKVLLPTYSYTTEREGDIFQEDFSGTWHKPCSTLFYGLKLRYGQGDSI
                     LYNDYGEVVCFESSELLNEEIGFFINKEIFLKYLNEQGYSVFWTMLAEKRVLEEPYMD
                     RKYIQPTISGVYTLNGENRLVGETIESEY"
     gene            complement(14457..15371)
                     /locus_tag="PC414_002796"
     CDS             complement(14457..15371)
                     /locus_tag="PC414_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HDF2465183.1"
                     /translation="MELVKNIHKNKSKDEIFKELLENLKGTIASWDYYVNWNKALENT
                     RKIEVQLNILNYLIGKEDIENETKNLLKEYPEVLSVIPIVIASRDNEFKILDPSDEDM
                     FKYKLFNFKKRKSISDEESELATEFLKKIKFLDLLKQKNIKNLVDYVFGVEVGLDTNA
                     RKNRTGSSMETLVERYVKNMCEKNNWSYLTQATVKKISEKWSIEVPTDKSKRKYDFAI
                     NNNGKIVLVEVNFYNGGGSKLKSVANEFTKLDKYIKDGGFTFCWITDGLGWLESSIPL
                     RDAFDKMDYIFNLDMVDKGSLEYIIKNL"
     gene            complement(15390..16304)
                     /locus_tag="PC414_002797"
                     /pseudo
     CDS             complement(15390..16304)
                     /locus_tag="PC414_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909111.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
     gene            complement(16732..17081)
                     /gene="ssrA"
                     /locus_tag="PC414_002798"
     tmRNA           complement(16732..17081)
                     /gene="ssrA"
                     /locus_tag="PC414_002798"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(17239..17694)
                     /gene="smpB"
                     /locus_tag="PC414_002799"
     CDS             complement(17239..17694)
                     /gene="smpB"
                     /locus_tag="PC414_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HDF2465184.1"
                     /translation="MAGKKILATNKRARHEYFIEETYECGIELKGTEVKSIRQGKVNL
                     SDGFASVDNSEVFLKQVHISPYEQGNIFNVDSLRVRKLLLHKHEIRKLIGATTIKGYS
                     LIPLSLYLKNGKVKVEIALAKGKKLYDKRQDMAKKDAQRRIEREMTGKY"
BASE COUNT         6066 a         2994 c         1826 g         7087 t
ORIGIN      
        1 aaaattaaag agagcaattt tttttgctct ctttcaaaat ttatattaaa ttgtaataat
       61 aaaaataaaa acgctagtta tctaaggcta atattttcaa cgattatata aatatctaca
      121 taacagttaa attacatact cttcttcagt tattgcaact tcctcagtga catatatttt
      181 ttctatttta ttaagataat ctactatact taatccaata gcctttccta gcttaacagg
      241 aactgcattt cctatctgct tgtacacctg actaactgac cctgtaaact cccactcatc
      301 aggaaaactt tgaattctag catactctct agttgtgaat ggtctagtct catctggatg
      361 acacctttca gtttgctttt gtgcaggact acatgttaaa gttaggcatg gttcatccca
      421 acttattcgc cttgccatac ctctttttcc gccaccgcta taataacttt ttcccatata
      481 atctcttgcc acctcatctg gcaaatctat ccaacaccca cctggtggta ccatatctaa
      541 cacttttttc ttcctctcag gatatctagc tccttccgat tttggaacat ttttaagtgc
      601 atctcttaac gtagcaacat attcaaacga cttagggaac ttaaaatcca catgtgacaa
      661 atcttttctt gttcctatta taataactct ttccctcttt tgtgcaactc catagttcac
      721 agcctttagt atctcatatt ttacattgta tccaatctcg ctaaatacat ctatcatagt
      781 ctttaatgtt tttccaccat catgagatac aagacctcta acattctctg ctaaaaacac
      841 cttaggcttt aactcttcta gaattttagc atatgaataa aacaatgtcc ctcttacatc
      901 ctctaaccct agcttcttac ccgcatagct aaatgcttgg catggataac cacctgatag
      961 caaatctatc tctatattat tatcaatata attctttatt ccattttccg ctattttaac
     1021 aatgtcaccc tgtataacat tccagtctgg tctatttaat tttaatgtat cacaagcata
     1081 tttatcccat tctactaatc caacctcttt aaaaccagct ttttcaaggc ctaatgctaa
     1141 tcctcctgct cctgcaaata attcaacaca tgtatatgct cttttagcta gtacattact
     1201 tatatctata tgtttattat ttttatttct ctctctagga tttaattcat attcactgag
     1261 ttctttatat gtacagtctc tatgctctat ataatcttta ttactgctac aaatatttgt
     1321 aaatttcagt tgcttttcat ttacatcttc caaacacata atttcatcga atccaatatc
     1381 taacacttca cataattttt ctacattact ttttataggt gtaaattttt ctgataatat
     1441 tgcagataac tgattcttag ttatgcccat atttaatgcc agttgattct gacttcttat
     1501 atttttctct ttcataattt ttctcactct atttttatca atagttattt ttttcattta
     1561 gttcacctct taataaataa tataccctta aatagttaat aaaatacatt aactatttaa
     1621 ggatatatta tcataattct aatttttatg taagtagtta taaaaactta aaataaaata
     1681 tctgtaaaca acctaatatt acctcataat tttacacatt atggaatata ttgtataatt
     1741 acattaatac tttttagggg gattatttta tgactgaatt agaaaaacaa gaaatactag
     1801 ataaagccaa agattttttt acaaatattt tggttgctaa tcataaaaag aatactatta
     1861 agttaaattt aaaggcattt aatataaatc cttttttagt taaatactta gctagtttct
     1921 taacaggatc aaataaacct gaagatattg ctaaagcatt gatttatcca agagttctag
     1981 gcacttcgat taatacgtcc ttcggaacta atttacaaaa attctgtatt accactttag
     2041 atggttttgc ctctacaact tctggcattg atatagagtt tattgattgt attgatggta
     2101 gaaggaaatt ctgtcaaatt aaagctggac caaacactat taataaagac gatgttaaaa
     2161 ctataattga ccactttagc tcagttaaaa gacttgctag aacaaatgga ctttcattat
     2221 cttataatga cttaattgtt ggtgtttttt atggaacaga aaatgagctt aatgctcact
     2281 ataaaagaat taatgaagag tatccagtag ttgttggtca agatttttgg tataggctta
     2341 ctggtgatga aaatttttat tttgatttaa tagatacaat tgctcaatct gctttagaag
     2401 aggactgtac tgttttactt aaaaaaacaa tatccaatct agctgatgaa atttctaaag
     2461 ataaatcttt attcaatcaa aagtaatata aaaatgatta aatagcataa tttacatttt
     2521 tagatattta aaaataatat tcaccaaaca aaattattaa gtaaaatgaa atgtatctta
     2581 taaaagctga gctataagta aatctttgta aaaacaaatt tacttatagc ttttttatgt
     2641 tcaaaaaaca caaattatat actatttaat atattataaa cttttatttg ttaatactct
     2701 ctactaaatt tctaacatct cttatataaa ttaactattt aataattcaa tcataaatct
     2761 agctcctgcc ttaaaaccaa gcttataacc aagctctctt tcaatctcta taactgaatt
     2821 aaataaatca tttaactcta tcaatacatc cattttcttt tcttctgata aatttttttc
     2881 tagtaattta ttaactttct taatttcttt acatctatca ttataatact catctttttc
     2941 tagtactttc ctatacatgt gttcatttat ccaataatca aaatatattt ctataatttc
     3001 atcatacatg ttagtacctc cttgactttc atctggtact taatatatac ttaacttgtg
     3061 tagattagtg tacattaagt acctaattta taaaggtagt tgttatattt atatggcaac
     3121 tacctttttc taataaattt ttcaatctaa atactatata ttttaaatct tcattgctta
     3181 ctattgagga ttatatactt ttcttctaac actccttaaa caatagttta tatatcactt
     3241 aataaattat attttttcat acctccttta ccttcttaca ttccttaaaa gaaaacaaac
     3301 ttcctatata ccaataaggt ctaaagcctg attccccatc tctattttta gcaactataa
     3361 tttcacttaa attagctcca aaaccttttt ctctacgtct taattctttt atatcctcta
     3421 ttcccattgt cttacaataa tcaagagcct ctttctctgt aggtctatga atatatatga
     3481 ctgaattaga atccatatat atctgttttg aacttctcat tactctatct ccttttggcc
     3541 tttcatcctt caattcatca tttaattgag ccaactgtat aattggaata ttaaagtcta
     3601 aagtaatctt ttttatttgc cttgataaat cagcaaactt agcttctcca acttctttac
     3661 cttcgtaatc caaaatctga agataatcaa ttataacaac atctggttta atacttttta
     3721 tttctctctt aattcctgat attgtattga ctgaatcatt aatatagaga ttattttcct
     3781 gatttaaagt acttaaagca tttatgtatt taattatttc ttcttcattt agatttctat
     3841 tcttcatttt tacaactgat attccagtta aagaagctat gtttctaaac aaaatttgta
     3901 tatcactcat ttccccactt ataaaaagta cttttttacc ttgaaatacc acatttctca
     3961 ttatctgtaa ggctagtgct gtttttcctc ttccactttt ggctgctatt gtaattaatt
     4021 caccactata tagacctcca atagtttcat ctaaaaattt tattcctata tcgatacctt
     4081 cttcaatttc atcatttatg tattcaaagg ctctttctga tatagctgtt atactagaat
     4141 ccttcttaac caatttctga ttaacttcat taacgtattt ttctaattga tatataagtg
     4201 tattatcatc tatttcacct aatttgaatt tattaaattt atcaattata tttctatttt
     4261 tatatttttc aataactatc tcaatataat gggatacatt acctatagtt ggaactattg
     4321 ttgatagttt tgtcaaataa cttatacctc caacataatc tataagcccc tttttcttta
     4381 aatcttctgt aagagttatt atatctatag gctcatccct tttatgtata tctttcactg
     4441 aattgaatat atgtttgtgc tcttgatgat aaaacatatc ttcacttatt aatatctcaa
     4501 tctcttctat tttcctagaa tctagtaaaa tactacctaa tatagattgt tctgcttctg
     4561 tgttcttaac taaattactc atattatcca taatataaac tccctaccac tttaattgtg
     4621 aatcatctgg actatatgct tctgcatcta ctttgacatt aattgattta tcttgataat
     4681 tgccttctaa tatcttaata aaattatttg ggctcattat ccaatcaaaa tttgccttcc
     4741 aacctttttc attgcttcca tttaaaaagc tagttttact aactatatga ataatttcta
     4801 atacagtctc tttatcatat tcttttattc tagcattaat tacttttttt ctagcttctg
     4861 taagtttctt tacttttgat agttctgtac aagtaatatt gaatttttct ataatttcac
     4921 tgtatatttt acttttttca ttatttacat tcttgttagt tgttagttgc ttgttaattg
     4981 catgttgatt agtctgttga ttttcttcat tagattgata tagctcccag ttttcaatag
     5041 ttataagcct atttttgttt gttgatttat ttgttagaaa tccatatctt tcaaatttct
     5101 ttaaagcagt tcttacattc tgaatactta catcttttga acacatattt gctatacttt
     5161 ccaaagatgt tattaactgt cctgatttaa cttcatattc tttacctcca taaacccatt
     5221 tattaggctt atgattaact gacagtaata ggtttatgag tatatctctt tgtttggaat
     5281 tacaatcttt atatagtggg ctttctttta tctttctatg aagctttatc caacctagat
     5341 tactcaaata ctatcaccca cttaagtcta taattttata ccatttgcat tttcaataaa
     5401 ccaattatct agtttatttt ttattatata cactctatta tctatccata tacaaggaaa
     5461 tcctttattt ttagaatgac ataactgtct aaattttgat tcccctatgg gataaatttt
     5521 agaagcttct ttacaagtga taagttgagc tttatctctt ctatatgtct gtatctcctt
     5581 gtaccctttt tccttttctt gtgcttcttg ttcttgcttg acaagtatat tcaatgtagc
     5641 ttgtagtgtt ttaagaactt ctagattatt tgtatctgcc atacatcatt cctccttatc
     5701 aaaaaataat tcttctacac ttgtatctag ttcttgagcc aattttaaca tcaacatatt
     5761 acttggattt tttgatttac ctctttctaa agaagataaa taatatcttg agatgccaac
     5821 tttttcagct aattcatatt gctttatacc tttttcaatt ctttttaatt tgagtttcat
     5881 tgatttcctc ctttctttca tacccatttt gtgtatcctc atttctataa aatattctaa
     5941 cacccatttg gtgtattgtc aatatattta agtgtatcaa tagacccatt tagtgttata
     6001 atgtatttga ggtgaaatta tgatagataa tacaattgga aaaaagatta aacaatatag
     6061 aaaatctata aatatgtcaa gggatgagtt agctaataag attggttgct cagtttatgc
     6121 tgtagctaaa tatgagcaag gccaaagaaa gcctagttta gatatgataa aaaaaatatc
     6181 tactgcatta tcaattccta taaataggct tattattgat gaaagtgatt tagatacttc
     6241 aacaaataat tatactaatt taaaagaatt ggatttagtt gtaaaaacat ttgataatta
     6301 ctcagcaaaa ctcatcggaa ataatataag caaatttaga aatataaata atatatctct
     6361 caaagaactc tcagaaaaaa taaattgttc tgagaatgaa attataaatt atgagaatgg
     6421 aacaaaaatg cctagaattg aaatattaaa aaaaatagca cttgctactt atactaagat
     6481 tgaagactta tttggatttc cattagaaga gataaaaata gataaccaaa caatcctcta
     6541 tagaaatccc cagactggat atattataga taactttctt atcgatttta taaataaaga
     6601 tattaacata gataatactc cagtttctta ttatatagat aagtcagaat ttgatttagt
     6661 aattcatata ttaaaaaatt atggttatga tgtaaaaaca gattataaga caaagtttga
     6721 aattgcagat aatgcagata caataaaacc aattagctta agcttctctg acttcaaaga
     6781 cttctccaaa aatctaaatt gggtgataaa tggtcttgtt gaaaatttta tacaagaaaa
     6841 ctctaatggt cttattgaag atgaggatga agaatattag tattgcacat tttcaaaata
     6901 tgagtttacc tattaacttt tatacattca tagttaataa ataaaaatta gatatgatat
     6961 gaaaggagct aaaatcatga ataaatcata tataccaaaa tacacacata aattccctac
     7021 taatctacct catggagata aaaaatatac tttaacatac attagaagaa tgataagtga
     7081 atttgtatat gatatgggta acctagaaaa taaccccttt acattcccag aagtacaaac
     7141 attacttgat ggtataaccg taggtggaca taaattaagt gaccaaaatc aaatactaaa
     7201 tataaaatca tcatgggatt atgtaataaa attaagtaac aaagaaaact tatctttaaa
     7261 taaagataca atttgctcta tacataaaat agttgcaaaa gatgaagctc taatagttgg
     7321 agattttaga aatggtaaca taggtatagc tggtactact caatatgaat gcatagaagc
     7381 cactctatta aatgatttat ttgtaagcga cataaatatc ataaataaaa taactaaccc
     7441 cttagaaaag gctataataa taaatttatg gttgtcctat tgccaattct tctatgatgg
     7501 caacaaaaga actgctagac ttacctcaaa cctaatactt ataagtaatg atataggagt
     7561 tctatcaata ccagctaaac ataaacaaga atacaacaca ttaatgctaa acttttatga
     7621 aacactagaa gctgatgaag ttataaaatt cttattagag aaatgtatta cattctttga
     7681 tgaatataac tataaatcat ataaggagct gtttaaaaat ggcaactaaa agagctaatg
     7741 gagaaggctc tatcggaaaa tataaagatg gttggcgttc ccgtataatg attggctata
     7801 atgaagatgg taaaccaata agaaaagaat tttatggtaa gactcaaaag gaagttaaag
     7861 ataaacttga tgcttataaa aaacaatatt atttaagctc tgatatttca gatgataaaa
     7921 taactttaga gcattggttt tacaattggt tatttgaata tagaataaaa gatttaaaac
     7981 ctaaatcatt tgaaagatat gaaagcatct atcgtaatta tattaaagat actgatttag
     8041 gcaatgttaa attaaaggac ttaagagtat ctcacataca aaaatattac aatagattgc
     8101 ttgattccaa taaaagtata cctactataa ggcaaataaa cactaaactg aaaacctgct
     8161 tatcagaagc tgaaaaacaa ggtcttatac aacgtaatta ttgtactatg gtaaatatac
     8221 cagttgagaa aaaggagaat aagcttgaaa tattgactct tgaacaacaa aagagtttta
     8281 taaaagctat agatggacat aaattagagg tattattttt acttgctctt ggtactggac
     8341 ttagattagg agaattatta ggacttaaat ggtttgatat tgattttaaa aagtctaatt
     8401 taacagttaa aagaaccctt caaagaacat attttataga taaaaccgga aatagagagc
     8461 ttaaagtatt agagcaagag ccaaagacat caaattctta cagaactgta cctattccaa
     8521 aagatgtttt aaataaattg aaagagcata aagtaaatca aaattcggat atattaaagg
     8581 ctggagaatt atatagaaat gataattatg tattttgcaa tgaattaggt gcccctattg
     8641 atgataaaag acctttaagg aatctaaaat ctatattaaa cagcttagat atagagccta
     8701 taaaatttca tggtcttagg aaaacttatg cgactagact ttttgagaat gatgtccctc
     8761 ctaaaactgt acaagtttta atgggtcatt atgatatatc aataacttta aatatatata
     8821 ctcaagttat ggaagataag aaaattgaag ctgtagaaaa gttggataaa atattttcat
     8881 tgtaaaaatc aaatatataa aaatactctg atttcatata gttaattaca aaaccaaaat
     8941 aaggatacaa attaatttta tattatcttg tatccttatt cttgtctagt tttttaaata
     9001 tttatcttgg atagccttta tgatacatta atccattttc taacattagt tcttgggcta
     9061 aatatatatg acccttacag tgcatatctg atataccctc acttataagt ttatatgttt
     9121 cagaagtata taaataatcc atactttctt ccaagctctt acctgtttgt ttagaaaatt
     9181 ctaatatgat ttttttatat agcgattgta acaatgtctt attagcttcc ataaaagcta
     9241 tacctcctct ttcaaatatt tgtttatcct attttgacta actatatatt tattagttat
     9301 ttaattagcc ataataattc ggctataaga tatttataat ctaatctcaa ttatataaat
     9361 ctttttgcca atatttaata atcaaaatat tgcacttaat taactatctt ataagtattg
     9421 ataataaaat ctgtcttctt aaatatactt aaataaaact tctataatta tatcaaagta
     9481 ttaatattca ctctctattg tctctcctac aagtctattt tctccattta gggtatatac
     9541 tcctgatata gttggttgta tgtattttct atccatatat ggttcttcta aaactctttt
     9601 ttctgctagc atcgtccaaa atacactata tccttgttca ttaagatatt ttaagaaaat
     9661 ttccttatta ataaagaatc ctatctcttc attaagtaat tcactacttt caaaacaaac
     9721 aacttcacca taatcattat atagaatcga atctccctga ccatatctca atttcaaccc
     9781 ataaaataga gtactacaag gtttatgcca tgtgccagaa aagtcttctt gaaatatgtc
     9841 accttctcgt tcagtagtat aactataagt tggcaataat actttataat ccttttctaa
     9901 gatattctta tgttcatcta tttgtaccca ctcttctcca caataatatg aattttgaaa
     9961 aaagtaatat ccatctgccc aataatattc cttattataa agagtataat tactattagg
    10021 ttcagtaagc catctaccca taaagtcttt tccctttaga gcattcacta ttggttctag
    10081 ttggtcattt ttaattatat agctattgat taatacccac atgtcttttt caggattttt
    10141 atagttctta tttttaaaat cattttcctc cttccaagta taccatccat taagtaataa
    10201 aaagtcttga tttttgtaaa cactatttac catatcattt atgcttggta aatcactaaa
    10261 atctcctaac cactcattat taggtattgg agaaaattga aataattgat tatgtactct
    10321 tttattgtta ttattattta ttactgtcat gacagttgga tcaatattcc taatatctgg
    10381 ctcaaatgta cccttacaat aaatttcttt tttagtacca taagcatctg tatacacttc
    10441 tatcttataa ttatcagcag cttgcgctgc aagttcatat aatgcaatcc attgatattt
    10501 ctttcctatt ctttcatatt tactatctct taatctacca ttttgtactg ttctatcaaa
    10561 tttaccatgt ttatttacat catatcccat atcaaaaact ttcttaatag ctatattctt
    10621 taaatcgtta tagtctagac ctttccattt atggaaatag tgttggaaca tatatcttcc
    10681 aaaatctcca taccatcctg gttgtccatc tctagtatat tctacttcca tagaatttaa
    10741 gatagtgttt tgactccaat aataatcttt aaaatctgat gccttatagt catacttata
    10801 tccctcaatt tcttcatcag ttggtacttt aggaaaaatt gatttatatg gtggttcaac
    10861 attcatatct tttaatactg aactatcaat tttatatttt gcataatcta caatattttt
    10921 tgcataatct cttaataaaa tattgggata aacataatct acatcaaata ttttattata
    10981 aacataaact gccaatgcct ctatcatctc ttctgattgc tctgatacaa cacatccaaa
    11041 tgcaactgca tataatcgtt caataatata tgggtcatct acatcttcaa atgttttaag
    11101 taaatctatt acaatatcca ttttccctgt taatagattt acaagagctt ttgtactttt
    11161 atctcttaag tctctatttg aagaaattaa aaacgttgca atcatcatag ctgacaatct
    11221 aattgtttca tttgaagtat tatcaaaacc ttcaaaatca aagcaccatc ctaataatcg
    11281 cttaatagaa gactcttctt cttcataaac ttcattaaat ataggtataa agatagcatc
    11341 tctatctgcc atagtatttt gtaaaatata ttttactata taatctgcat ttaatgtatg
    11401 attaaccttt gtagaaatta agataagtac attataaaaa tctattttat aataagattt
    11461 acctaaaact attttattaa tataatctag tgttttttca gtaattgtag atttcttacg
    11521 ccattttagt gcattgataa atgcactaat aacattataa tttgtttctt tatttttaaa
    11581 aacttcaaaa atttcattag gtatatctga gcctgtctct tcagataaaa caattgccaa
    11641 tacttctaat atatcctcat attctactaa atatttatac ttatgtgaaa aatctactac
    11701 cccaatttct tttaactctt taaccaacaa atttgcatat aaataatctt ctaacttctc
    11761 atatgtcaca tgcatatatt cttcatcatt taaattgact gttttattaa ctattccatt
    11821 tgctatgagt tcatctatta tcacattgtt aatcttatac ttacttgcaa tatttattat
    11881 aatctctaca ctcttgtttg cgggtattaa attattccca agaccatctc catatttata
    11941 tttaatcatt tcattaagta cttctgtaac tacattggca tgtctactat aattgcatga
    12001 ttcagatatt ctaagattaa gtatattcaa ataattttta taaatatctg taaaactaat
    12061 agtatctaag ttaatctctt ctcctttaaa agtttcacac ataattctca aaaaaagagg
    12121 gtttctaaat tcttgatttg gcattggaat attagaataa ggtattccat aataatcaaa
    12181 atactttttc atagctttat gttctatact agaaaatcct tgatgctcta taaaaaccaa
    12241 attctctttt atcttgttat tattatctat taaagatggt atatattcac ttcttattga
    12301 taatattagt ccaatccatg gatatttctt tatctgttct acaataccat ttaaataata
    12361 tttccaaagt tcttttcctt gaccttcatt caatgcatct ataaataata tcacacgctg
    12421 ttgctctttt tctcctattt cattaagtat ttctaataat tcttcaaact cacactcaat
    12481 ttctaatatt tctaacatat gtttaattgg tgttttatcc aacttaaaat gttgccctaa
    12541 aaataatata gacttatgcc catccatatt tcttttctca atagtatgtg caattatatg
    12601 agatttcccc ataccacctg cactatgtaa taaaatataa gggctattaa ctacttttat
    12661 ttccttagaa tccaaatatt ttatatatac atagatatct ttttgagtct gcttcaattt
    12721 gtatatttca tcttctcttt ttttactttt ttcacagtct tcatttataa ttatattttt
    12781 aaaattattt attctatcat caagtgctgc acttattttt tcaaagtgct tttttaacaa
    12841 cttgatatct aatctttcat tagctttgtc ataaaattgt tgtattattt cttctataac
    12901 tagtacatct acttccactt catttatttt ctgtttcttt atacttgata gaactttatc
    12961 tgttttttca tcaaaacgtt gtttaaattc ttcatttttt cctaatgcat taaacgtatt
    13021 ttctaaactc aattctacat tcaattctgg taaatatcta tctccaaggt tttttatctg
    13081 tatattattc tgattttgaa accatttctc tgttaatcta caagataaat tagttaacct
    13141 actttccttt aaagacctat cctttaatct tctatattca tctggcaaaa ctatttccaa
    13201 aattcgttca agtattattc ttacacactc atgcttacct tcaggataca taatggtttg
    13261 tatataatac tcattttcta actctattcg tctatgtttc tttttctcac tactaactgg
    13321 aataatagca taatgacccg ttcctgcctc tgatacttgc tgtaataatt ccaacggtct
    13381 atccttttcc aaactacatc caagaaataa caactcttta cttgtatata cttgtgtaag
    13441 catctttctc aattcatcat tactatatgc tttttcatat tgttcttttg ttaacacaat
    13501 agatgttgta ggctctgtaa tatccccatg aattttatac aatactaatt cattatttct
    13561 aagtgcatta tttaatgctt caaagtgacc tggatgtgac actaaaactg ttttattgtg
    13621 taatccataa tttttttcta gtactttatc ataattagta gttataatta atcctgagcc
    13681 aaaaattata ggaattaaat agacacattt atcaataaaa tcgtctttat cttttgcatc
    13741 cagataacct gccccaaaaa ctttttttaa atgtttagaa aatctaactt tcgccaattc
    13801 ttcatataaa aattctgctt ttttctcata atcctctatt ttttcaaata aaattttatc
    13861 ctcatcttct aaatattctt cacttgtgtt atttaaaaat gtactccatg aaggatatat
    13921 gtctttaaat aacatagaca tgcctgctcc aatatatggg actacataac catctttaat
    13981 tgcatttacc agttcatcaa aatttttttg attgtaaggc ttattaaatt ccataatttc
    14041 ttcaaatgtc attattccct cctaaactat ctcttaataa tttcaaccat aattcctatt
    14101 ttataatttt attatatcat tccattccat ttttttacta caattctatg ttatagaatg
    14161 aattcactta cttacatttg ttaataatat aaaactttct tttaagataa ttaaaattta
    14221 tacttttaaa ttatggataa aaatggaatg ttataacttt taatgtatta ctaatattaa
    14281 caattttaaa aaataatact attcttattt tatgctataa ttatattttg cataaatatt
    14341 attgatattt tataaatata attatactta tatctcatac tcaaatattt tatataatat
    14401 ataagtctaa attaacttgc aatataaaag cgcctcttaa tataggcgct ataatgttat
    14461 aaatttttaa ttatatattc caatgaacct ttatcaacca tatctaaatt aaatatataa
    14521 tccatcttat caaatgcatc tcttaatgga atacttgatt caagccatcc taacccatct
    14581 gtaatccaac aaaatgtaaa acctccatct tttatatact tatctaactt agtaaactca
    14641 tttgcaacag attttaactt agaacctcca ccattataaa aattaacttc tactaaaaca
    14701 atttttccat tattatttat agcaaagtca tactttcttt tactcttatc tgttggaact
    14761 tctatagacc atttctcact tatttttttc actgtagctt gtgttaaata actccaatta
    14821 ttcttttcac acatattctt tacatatctt tctacaagag tttccatact acttccagtt
    14881 ctattttttc tagcattcgt atcaagccca acctctactc caaacacata atctactaaa
    14941 ttttttatat ttttttgttt taataaatct aaaaacttta tttttttaag aaattctgta
    15001 gctaattcgc tttcttcatc acttatactc tttctcttct taaaattaaa cagtttatat
    15061 ttaaacatat cctcatctga tggatctagt attttgaatt cattatctct actcgctatt
    15121 actattggta tcacactaag aacttctgga tactctttta ataaattttt agtttcgttt
    15181 tctatatctt cttttcctat aagatagttt aagatattta actgaacctc tatctttcta
    15241 gtattttcca aagctttatt ccagttaaca taataatccc agctagctat agtacctttt
    15301 aaattttcaa gtaattcctt aaatatttca tccttactct tatttttatg aatattctta
    15361 accaattcca tttgattccc cacctcttca taattagtaa ttagcaattc agttataaaa
    15421 cctcttccat tccccttaga atttatgaat cttttagcat gtactctttt tatattaaaa
    15481 tctgaataca aatcatcaaa gaaagtatct tcttcatcta aaacttttgg atcagaatta
    15541 cttaacatca attttgtatc tattttatct agaactttat aaaatttact taactcaatt
    15601 tgactatcat cattaaaatt acctttgtta taggaattaa atcctcctat agttactggt
    15661 ctatatggtg gatctagata cacaaaacaa tttttatcta ttatattttc tagatttaaa
    15721 taattcttat ttaatatttt tacttttagc tctcctcgag aatctctttt atttaaaagc
    15781 atggataatt tttcaagttc atttttatca aaaaaactag gatttttata tcttccaaaa
    15841 ggtacattaa attcacctcg agaattttct ctatataatc cattaaaaca tgttttattt
    15901 aaaaatataa aatatgaagc ttgctttata caatcttcat tttctatatt tttttcattt
    15961 gtaagtatat taaatctatc tctaatatca tagtacatct tttctctatc tgaagaacct
    16021 tcttcgtaag atatgtattg ttcttttaaa ttagttagtt ctgttattaa ctcttccaaa
    16081 tcatctctga ctttaatata taaattaatt aacttactat ttatatcatt aattacatac
    16141 ttgttgaaaa tgtcctcttc taacaaatat agaaatagtg cacctgcacc tacaaaaggt
    16201 tctatataag tcatggaact aaaatcacaa cctgtaggca tttgttttct tagaatatca
    16261 agtagctgtg ttttacctcc agcccatttt aaaattggtt tcatgcaaca cctctctcag
    16321 ttatattgtt atataattat ataaaataag attctaaaat acaagtactc atcagaacac
    16381 tatatttata tcaaatcttt ccaaaatcta agtattattt tatcctaaca cttttcaaaa
    16441 cctctaaata atatacaata ttctctaata tttacttact aaaaatttaa atctatcttt
    16501 aagtataacc taaaacagat tataagttac gtagttttta atataaaata tacttctaca
    16561 agtaaagctt taaatatcaa ttacttattt cataattaat gactatgtac cctcaaaaaa
    16621 acctcatttt ctatggttgg ggtacggttg gtggatttca tcaccaaaat aaaaaatact
    16681 agtcaataaa gactagtatt tccaagtatt tcatcattct tttttcaaaa ttggtggaga
    16741 cgaggggagt cgaacccctg tccgcaaatc ttttccttgc tgcatctaca tgcttagcca
    16801 acttcactaa tatctcacct cacatagcgc caagtggcag gctctatgct tagccagtcc
    16861 caattaatct acttcaggtc gagacacccc taaagtagtt ccctgcataa tgacgtccag
    16921 tcttagtcgg caggaggact aagagggacg agcagcaact aggctgctat cgcgaaatta
    16981 tcgtctgcgt ttattttaaa gttccccagt ttttacgtga cccagagtaa cacgacatgc
    17041 agcctcaagt tccaaaccca cgtcgaaacc tttacgcccc cttgagtagt ttatagtcca
    17101 gtagactttt cgcaaacttt atcaccattt ttattaaatt tttaatgtat atataatata
    17161 atacttttaa gaataaaaat caatgttatg ttctagataa taaagaattt tattctaaaa
    17221 aatacagttt tatctagttt aatacttacc tgtcatttct ctttctatac gtctttgtgc
    17281 atctttctta gccatatctt gacgtttatc atatagtttt ttaccttttg ctaaggcaat
    17341 ctctacttta actttaccgt tttttagata caaactaagt ggtataagtg aatacccctt
    17401 tatagtagtt gctccaataa gctttcttat ttcatgctta tgaagtaata gttttctaac
    17461 tcttaaagaa tcaacattga atatatttcc ttgctcatat ggacttatat gaacttgttt
    17521 taaaaatact tcgctattat ctacagatgc aaatccatca cttaggttaa ctttgccttg
    17581 tcttattgat tttacttctg ttccttttaa ttcaattcca cattcataag tttcttctat
    17641 aaaatattca tgtcttgctc ttttgtttgt tgctaatatt tttttaccag ccatataaat
    17701 ctcaccttct ttgctagaaa tttattccat aaatattaga aatttactaa atatttaatg
    17761 ttgccaaatt atttgactaa cttgtatgat aacatattta ggcattttag tcaatatttc
    17821 actgatatat taagttagtt aggctatata taattaagta atataaatgt atgaaaatag
    17881 ctaaaaaact acttcgaata tacttaatac taaactaact ctaaccagta atactaattt
    17941 ataaatttca aaacaaaaat attacatatt ata
//
LOCUS       DAMJVK010000082        17669 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.083, whole genome shotgun sequence.
ACCESSION   DAMJVK010000082 DAMJVK010000000
VERSION     DAMJVK010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17669
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.083"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     misc_binding    222..453
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            561..2042
                     /locus_tag="PC414_002800"
     CDS             561..2042
                     /locus_tag="PC414_002800"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HDF2465185.1"
                     /translation="MYYSSTRGTEEKVTASQAIIKGISNDGGLYVPSEFPNVKNELIN
                     LVNLTYSQIAFFVLSKFLCDFTEDEIKNCIENAYDEKFDCSSIAPLNKVNDTYFLELY
                     HGPTLAFKDMALTIMPHLLKTSIKKDNLEKDVVILTATSGDTGKAALEGFKDIDKIKI
                     IVFFPEDGVSPVQKLQMKTQTGKNTYVVGIKGNFDDAQSGVKKIFSDKEFNQKLLDNN
                     YILSSANSINIGRLLPQVAYYFYSYMKLVSNSDINLNDKINFVVPTGNFGNILAGYYA
                     KQMGLPINKLICASNDNNVLYDFFKTGVYDKNRTLKLTTSPSMDILISSNLERLLFEI
                     SNRNTDIVNKLLSDLSDKGVYKISDDMEKNLASFYGEYSNEEEVKSTINNVFKNYNYL
                     IDTHTAVAYNCYEKYKKETSDNTKTVIVSTASPFKFSEDVLKSIDCDFKDLDDFAIID
                     RLSSISKIDIPKPIKNLKNAEILHKDIYEKDELKLAIKKFLKV"
     gene            2052..2945
                     /locus_tag="PC414_002801"
     CDS             2052..2945
                     /locus_tag="PC414_002801"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HDF2465186.1"
                     /translation="MLEIIVPATSANIGPGFDCLGIALNIYNKFYVEEIESGLEIEGC
                     EDAYKNENNLVYTSMKYFFDRVKPEKIPAGIKIKIQSEVPICRGLGSSASCIVAGVIA
                     ANALSGANLDKNQLLNIASEIEGHPDNVAPAILGNMIVSVTDNENIHYDIIKIPEELK
                     FCAMIPNFKLSTEKARGVLPKEIPYSDGVFNVSRVALLISALLNKNFDLLKVACQDKL
                     HQDYRGTLIENYNDIVEKSEQLNSIGVFLSGAGPTIMSLIKENDDSFVDNMKNYLQKL
                     KSDWEIKELCCDSNGAVLNII"
     gene            3069..4130
                     /locus_tag="PC414_002802"
     CDS             3069..4130
                     /locus_tag="PC414_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HDF2465187.1"
                     /translation="MIIKELIIRDLVAKVPIIQGGMGVGISRSSLAGAVAKLGGVGVI
                     SGVQIGYDEEDFETNTINANLRAIKKHISKAKEISNGGIIGINFMVAMKEYETYVKEA
                     VKAGVDLIISGAGLPNKLPSLVKGSNVKIAPIVSTAKAANVILKMWDRKEKTTADLIV
                     VEGPKAGGHLGYSNEELDNIDSIDYDKEFVEILKVANTYGEKFGRNIPVVAAGGITSS
                     SDVKKYIDMGASGVQVGTRFVATYECDAHENFKMAYINASEEDVHIVKSPVGLPGRAI
                     RNKFIEEVKINRPEIKKCYNCLIPCNPKETPYCISQALINAVKGDVENSLLFCGNDAY
                     KIDKLSTVEDVINELISEL"
     gene            complement(4285..4797)
                     /locus_tag="PC414_002803"
     CDS             complement(4285..4797)
                     /locus_tag="PC414_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HDF2465188.1"
                     /translation="MSDFKEILAEEIKNNPFGLIGKDWTLITAENEGKVNTMTASWGG
                     LGVMWGKDVAFVVIRPQRYTKEFIDNTDKFSLTFFDEDFRKELSYCGKVSGREEDKIS
                     QIGFNIEHLNDTPYFKESKMAIICKKMYSQKLEPQCFIAEGIDGRWYPQKDYHTLYIA
                     EITNVLVKED"
     gene            5317..6252
                     /locus_tag="PC414_002804"
     CDS             5317..6252
                     /locus_tag="PC414_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDF2465189.1"
                     /translation="MIRYYKTIDSKLEKLSFFEDGCWINLVEPNHSEINEISNLLNID
                     VESIESALDEEERSRIDVEDNHTLILIDIPVDESDSNSSHYTTIPLGIILTEEAIVTV
                     CDAQTKILNDFIVGHIKDFFTFKKTRFLLQILHKNAAYYLHYLRKINKMTIIIEREIY
                     KSMKNKELVQLLELEKSLVYFSTSLKSNELVLNKMVRTAGIKKYPDDEDLLEDVIVEN
                     RQALDMAKIYGDILSRIMDAFSAIISNNQNNVMQILTVVTLIFSIPTIISGFFGMNVI
                     NMPFSNNPNGFWIILLITSIICIVITFFMSRNKLL"
     gene            complement(6344..8215)
                     /locus_tag="PC414_002805"
     CDS             complement(6344..8215)
                     /locus_tag="PC414_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HDF2465190.1"
                     /translation="MKNTKFSIKKIKNISNDPKVVHTKGALLEKMGKMESAIELYKSA
                     ALDNYVKSQYTLGNIYESKKQFKEAEKWYSMAYKSGSEDAAFDLGNMYYKLDGYEYAI
                     YWYEKIANLGYLQAQNNLGVCHFKMKDFVRCEKWLKTAADKNLGKACFNLGVLYTFLE
                     KDDEAYEYYKKGSVLLDDDAKYNLAILNGKKKDNKSTVSLYKQLYKSGHMKGCFNLGM
                     IMEINDNLEDAEKYYKKSADRDDVKSEYRLAYVYDRKEELGDAIYYYEKAIEKNHILS
                     KYRLANLYNRENEIEKAKTYYKMAAEDDITEAKNNLAGIYFEEKDYENAIKYYEDAIA
                     VGCKSSLENLGDLYYQNQDIEKAISYYSRIPNNVSCQIKLGNIYEDLNNIEEAISWYK
                     KASENGDTRSSYRLGCIYESLGNTKNARKYFEMASSKNHMNARIHLGRIYFREGKLEE
                     SKMMFDTPANENNVYAQHMVGLIYDMFYKDYVNSKFWYEKARAQGCVESIYNLGQIYL
                     KLNDDAEAEKYYKEGIKYGSKKCEYMLAGLYYKKSLDMYISLANEEYDNCEEIVEGFP
                     NLNIDFDEVLIPPFKLQEEVIDEEEYVPIYILNIKESLDSMLEGLETEMIIDEEHDS"
     gene            8405..9331
                     /locus_tag="PC414_002806"
     CDS             8405..9331
                     /locus_tag="PC414_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDF2465191.1"
                     /translation="MNFSNIFIQVAVLFIIILVGYFVRKFNLLDDHCTSKLSTLTMTV
                     FLPSMIISSMQINFDSKMIQKILLLLFTSLIMYIVSIIIAFLLKYILKCNDKKDLGIY
                     QYIVVFSNVAFMGYPVIEAVLGHEAIFYTAIFNLPFNLFSFTLGIYLLSKGSTSRGFS
                     IKSLISPATIAIVIGLFLFVTGLRLPQFINEPLEMLGSITTPISMIIIGSLLANSSAL
                     DCFVNKKLYIVTFIRLLVLPVIVYFILKGWINDKMILAIPVVISSMPAAANTAIMANQ
                     YDSNITLASQCVFFTTLFSVISIPFISILLLS"
     gene            9470..9616
                     /locus_tag="PC414_002807"
     CDS             9470..9616
                     /locus_tag="PC414_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465192.1"
                     /translation="MDMDLLFKIVFAIVAIGVSVKIIKTVASLAFKIALILLIILFVY
                     RMFL"
     gene            complement(9721..10245)
                     /locus_tag="PC414_002808"
     CDS             complement(9721..10245)
                     /locus_tag="PC414_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2465193.1"
                     /translation="MSKPIDITIRPIRREDAQAFNEIRRTYSVMRNTLALMSDRVDKT
                     EAMLASLGENDYMFVAETNNNGEKDVIGFAGLHVNSSPRLRHSAEIGISVAENYQGRG
                     VGKKLMEQLLDIADNWIMLTRVGLEVIVDNEKGLNLYKKLGFEIEGTKKYAVIRDGKF
                     EDVYIMGRYNKNLI"
     gene            complement(10298..10522)
                     /locus_tag="PC414_002809"
     CDS             complement(10298..10522)
                     /locus_tag="PC414_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465194.1"
                     /translation="MIIKLTKDNFNFRFKQYEGMFFHAEVVEGMESIRYKITKDKEGT
                     ELKEFGSTHIKDNMIYVPQVEAYIDLNSIK"
     gene            10719..11843
                     /locus_tag="PC414_002810"
     CDS             10719..11843
                     /locus_tag="PC414_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
                     /protein_id="HDF2465195.1"
                     /translation="MSKKTKYFSLILFPIALLLNFIASKVPNIVEKYYSQFIDKIIVQ
                     ILSKVSGIFPFSLYEITMYIIVISTFLFLCCTLSTIFNKKKKLNIYLKNSILNILSIV
                     SIVYFLFVVLWGINYNRIPLETTLINNYNLKNNTSIQSKQYSVKELTKLYKFLVIKAN
                     ETRKLTLQDKNGIVKSNTDYNGVINRAQLGYDNILDILPGVSDSYSKPKYIISSNLMC
                     YTGITGIYFPFTGEANVNIAIPDLYIPCTVEHEMAHQRGFASEDEANFIAYLTSIKHP
                     NIDFNYSGYILALNYTASALSKVDYNAYVDISAGISDSVRRDLKNESEFWQKYEGKIN
                     EISNEFNNSYLKANGVTEGTQSYGKMVDLLLTYYELYPYN"
     gene            complement(12317..13141)
                     /locus_tag="PC414_002811"
     CDS             complement(12317..13141)
                     /locus_tag="PC414_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465196.1"
                     /translation="MRYKKISSLPILVVALLITGTTTTFSLSTTNSEKTNVYLANTVS
                     TVTKKVSDIQSSKGLVYNENIPLFINNSKIQYDDTPYHWPSNVISLTNSSEKAIMDYE
                     ITCLAYDKNGKPLELYWDAQNVAADGEVGSVGFSPAGVDYGIVTGISPVSPKSYSHTY
                     RKMQQSPPQDIISMFEKQQGKAWVENWLKEWKQMEKEYAKQNAIAPGKNQNDAFLLFD
                     KWKQSTGEHGVKYIISCVKQVTFNDGSVWKNSAYENWLKSFQGKEVSNSVLENYYK"
     gene            complement(13489..14544)
                     /gene="ispG"
                     /locus_tag="PC414_002812"
                     /gene_synonym="gcpE"
     CDS             complement(13489..14544)
                     /gene="ispG"
                     /locus_tag="PC414_002812"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HDF2465197.1"
                     /translation="MSYERRKTREVSVGAVKIGGENPISIQSMTNTDTRDADATIAQI
                     KRLEEAGCDIVRVAVPDIKAAKNIAKIKSSVNIPIIADIHFDYKLALEAIEQGVDGIR
                     INPGNIGSIERVKMVVEKCKERNLKIRIGVNGGSLEKELLKKYGSATAEALVESAMGH
                     IKILEDLDFHNIVISLKSSDIYKTVDAYELISKKVDYPLHIGITESGSVHKGTIKSSI
                     GVGALLLKGIGDTVRISLTGDPVEEVIVGKQILRSLGLLNDKIKVISCPTCGRCNIDL
                     ISVVNEVEEKIGSMEKNITVAIMGCAVNGPGEAREADIGIAGGKGEGLLFKKGEIVRM
                     IDGNKLVDELLEEIEKL"
     gene            complement(14727..15731)
                     /gene="rseP"
                     /locus_tag="PC414_002813"
     CDS             complement(14727..15731)
                     /gene="rseP"
                     /locus_tag="PC414_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HDF2465198.1"
                     /translation="MTIIAALILFSIIVLIHELGHFIFAKRSGIKVNEFSIGMGPKIY
                     SVKKDTEYSIRALPIGGYVSMEGEDEEQISPNSFGNKSILQRFSTIVAGPIFNIILAA
                     ILLVPVFLYIGSPTTKLGKIMPDTPAQAVGLQVGDKINKINGNSVKTWDEVANIINTS
                     SGGELKLSITRDGSDKVVNVTPKNNNGKYEIGIQPQREKDFLGSIVNACKTTVDMTKQ
                     MLTFLGQMITGRVPGGIGNAVAGPVGVIGMVSDAARTGIINVVYLAAVISLNLGIVNL
                     LPIPALDGWRILMLLLEAVRGGKKLDPNKEGMINVVGFGALMLFMLFITYKDILRLFQ
                     "
     gene            complement(15744..16898)
                     /locus_tag="PC414_002814"
     CDS             complement(15744..16898)
                     /locus_tag="PC414_002814"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HDF2465199.1"
                     /translation="MKKISILGSTGSIGKQTLDVVREHRDKFEIVAISANSNIELLLE
                     QIVEFKPKYVTVFEENKALKLKEMLPKNIEIEVLAGMEGLKIISSLDEVDVLLTAVVG
                     MIGLVPTLCAIKKGIDIALANKETLVTAGELVMKEAEKYNVNILPVDSEHSAIFQCLN
                     GENKKNIEKIILTASGGPFRGKKKGELANITKNEALKHPNWSMGRKISIDSSTLMNKG
                     LEVIEARWLFGVEQENIDVVVHPQSIIHSMVQYTDSSIIAQLGCPDMRLPIQYALTYP
                     DRMESSFERMNFSKFSTLTFEEPDLETFPCLKLAYECLKMGGTYSSVLNSANEVLVSE
                     FLEDKIGFYDIPYYIEKTLEVHSSISEPTLEEILETDRWSRAYVANLIKK"
     gene            complement(17062..17265)
                     /locus_tag="PC414_002815"
     CDS             complement(17062..17265)
                     /locus_tag="PC414_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2465200.1"
                     /translation="MISYAPLWKLLIDRKIKKMEFVNISGISISVLGRLGNDKSVSMD
                     TMEKICLALDCKIEDVVEIKKGI"
BASE COUNT         6106 a         2728 c         2087 g         6748 t
ORIGIN      
        1 gatatttaat agttatcttt taattacttt cattaaaaac agcttcatca ctatgaaaat
       61 ctaggttttt aaacttttaa aaaggttaaa aaaatatttt tctaatactc aatttgatta
      121 caattttttt acaatttaaa aaatattttt tccaaatttt tatattaata cttgactttg
      181 tctgttttat aaactagaat aattaataat attaagaata ttgaaaggca ttgattagga
      241 agagtaaatc tgaatcgatt accagagaag gaaactacta gctgaaatgt ttccctaatt
      301 agaaggattg aaaactacct aggagcttct atccgaagtt atcttaatga ttttagtagg
      361 aatagacgtt tttattactt gcgttaaagt atcaagtgga ttttttaaaa tcaatttggg
      421 tggaaccgcg ggaaacagat tctcgtccct tttttaggga tgagggtctt tttttattac
      481 ctaaattcag tttttaaaat attctgtatc taatttaatt agctaattaa aaatttaaaa
      541 tatatggagg attataaaaa atgtattatt caagtacgag aggcaccgaa gaaaaagtaa
      601 ctgcatcaca agcaataata aaaggaatat caaatgatgg tggtctttat gttccaagtg
      661 aatttcccaa tgtaaaaaat gaacttataa atttagtaaa tttaacgtat tctcagatag
      721 cattttttgt acttagtaaa tttttatgtg attttactga agatgaaatt aaaaattgta
      781 tagaaaatgc atatgatgaa aagtttgatt gtagtagtat agctccatta aataaagtaa
      841 atgatactta tttcttagaa ctatatcacg gtccaacttt agcatttaaa gatatggcac
      901 ttactataat gcctcatctt cttaaaacat ctataaagaa agataattta gagaaagatg
      961 ttgtgattct tactgctaca tctggagata caggtaaagc tgcacttgag ggatttaaag
     1021 atatagataa aataaagata attgtattct tcccagaaga tggtgtaagc ccagtacaaa
     1081 aacttcaaat gaaaactcaa actggaaaaa atacatatgt agttggtata aaaggaaatt
     1141 ttgatgatgc acaatcaggt gtaaagaaga ttttttctga taaagaattt aatcaaaaac
     1201 tattggataa taattatata ttatcttctg caaattcaat taatatagga agacttcttc
     1261 cacaagttgc atactatttt tattcatata tgaaattagt tagcaatagt gatataaatt
     1321 taaatgataa aataaatttt gttgtcccaa ctggtaattt tggtaatatt ctagctggat
     1381 attatgctaa acaaatgggt cttccaataa acaaattaat atgtgcatca aatgataata
     1441 atgttcttta tgatttcttt aaaactggtg tatatgataa aaatagaaca ttgaaattaa
     1501 ctacttctcc ttctatggat atattaattt caagtaattt agaacgatta ctgtttgaaa
     1561 tatcaaatag aaatactgat attgtaaata agcttttatc agaccttagt gataaaggag
     1621 tctacaaaat aagtgatgat atggaaaaaa atttagcctc attctatgga gaatattcaa
     1681 atgaagaaga agttaaaagc acgataaaca atgtgtttaa aaattataat tatttgatag
     1741 atactcatac tgctgttgca tacaattgtt atgaaaaata taaaaaagaa acttctgata
     1801 acacaaaaac tgttattgta agtactgcaa gtccattcaa gttttctgaa gatgttctaa
     1861 agtctataga ttgtgatttt aaagaccttg atgactttgc tataattgat agattatcaa
     1921 gtatttctaa aatagatatt ccaaaaccta ttaaaaatct taaaaatgca gaaatactac
     1981 ataaagatat ttatgaaaaa gatgaattaa aactagctat aaaaaaattc ttaaaggtat
     2041 aggtgattat tatgttagag attatagttc ctgcaacaag tgctaatata ggtccaggat
     2101 ttgattgtct tggaattgcg ttaaatatat acaataaatt ttatgttgaa gaaattgaaa
     2161 gtggtcttga aatagaaggt tgtgaagatg cttacaaaaa cgaaaacaac ttagtttata
     2221 catctatgaa gtactttttt gatagagtta aaccagaaaa aatacctgct ggtatcaaaa
     2281 taaaaatcca aagtgaagta cctatatgta gaggtcttgg cagtagtgcc tcttgtatag
     2341 tagctggagt tatagctgca aatgctctat caggtgctaa tttagataaa aatcaattac
     2401 taaatatagc ttcagaaata gaaggtcacc cagataatgt tgctcctgct attttaggaa
     2461 atatgattgt atctgttact gataatgaga atatacatta tgatattata aaaattcctg
     2521 aagaacttaa attttgtgca atgataccta attttaagtt atcaacagaa aaagctagag
     2581 gtgttttacc aaaggaaata ccatattctg atggggtatt caatgttagt agagttgctt
     2641 tactaatatc tgccttacta aacaaaaact ttgacctact taaagtagct tgtcaagaca
     2701 aattgcatca agattataga ggaactctaa ttgagaacta caatgatatt gttgaaaaat
     2761 ctgaacaatt aaatagtatt ggagtatttt taagtggagc tggacctact ataatgtctt
     2821 taataaaaga aaatgatgat agttttgtgg ataatatgaa aaattaccta caaaaactaa
     2881 aatctgattg ggaaataaaa gagttatgtt gcgattctaa tggagctgtt ttaaatataa
     2941 tataattatc gtcttctata caaaatataa ctactctttt attataattt gtagtataat
     3001 aattaggact aggtaataat attaagtatt ataatctagt tctttaaaca tgtttacatt
     3061 gaggagggat tatcataaaa gagttaataa ttagagattt agttgcaaaa gttcctataa
     3121 ttcaaggtgg aatgggcgtt ggtatatcta ggtcatcact agctggtgca gttgctaagc
     3181 ttggtggtgt tggtgtaatt tctggagttc aaattggata tgatgaggaa gattttgaaa
     3241 ctaatactat aaatgcaaat ttaagagcta taaaaaaaca tatttctaag gccaaagaaa
     3301 tctcaaatgg tggtattatt ggtattaatt ttatggtagc tatgaaagaa tatgaaacat
     3361 acgtaaagga agctgttaaa gctggtgtag accttataat atctggagct ggattaccta
     3421 ataagctacc atcattagta aagggaagta atgtaaaaat agcacccata gtttcaactg
     3481 caaaagctgc taatgtaatt ttaaaaatgt gggatagaaa agagaaaact actgctgact
     3541 taatagttgt tgaaggtcca aaagctggtg gtcaccttgg atactctaac gaggaacttg
     3601 ataacataga ttctatagac tatgataagg aatttgttga aattttaaag gttgcaaata
     3661 cttatggaga aaaatttggt agaaacatac cagtagttgc tgctggtggt ataacttcta
     3721 gtagtgatgt taaaaaatat atagatatgg gtgcaagtgg tgtacaagtt ggtacaagat
     3781 ttgttgctac ttacgaatgt gatgcccatg agaactttaa aatggcatac ataaatgcta
     3841 gtgaagaaga tgttcatata gttaaaagtc ctgtaggtct tcctggacgc gccataagaa
     3901 acaaatttat agaagaagtt aaaataaatc gtccagaaat aaagaagtgc tataattgtt
     3961 tgattccttg taatccaaaa gaaactcctt actgtatttc tcaagctcta atcaatgcag
     4021 ttaaaggaga tgttgaaaat tcacttttat tttgtggaaa tgatgcctat aaaattgata
     4081 aattaagtac tgttgaagat gttatcaatg aattgatttc tgaattataa tttaaagaaa
     4141 actaaattaa aattttatca agctaaggag tttaaatacg ttatttatta atctttataa
     4201 acatatttat gtccttagct tttttgtgtc aatttccttt tatggaaatt ttaatttaat
     4261 tagagatagt attaaccttt ataactaatc ttcttttaca agtacattgg ttatttcagc
     4321 tatatataaa gtatgataat ctttttgtgg ataccatctt ccatctatac cttcagcaat
     4381 aaaacattga ggttcaagtt tctgactata catttttttg catataatag ccattttaga
     4441 ttctttaaaa taaggtgtat catttaagtg ttctatatta aatccaatct gtgatatctt
     4501 atcctcttct ctaccagata cctttccaca atatgaaagc tctttacgaa aatcttcatc
     4561 aaaaaatgta agagaaaatt tatctgtatt atctataaat tccttagtat aacgctgtgg
     4621 tcttattact acaaaagcaa catcttttcc ccacataact ccaagccctc cccagctagc
     4681 agtcattgta ttcactttac cttcattttc tgctgttata agtgtccaat ctttacctat
     4741 caatccaaat ggattatttt taatctcttc tgccaaaatc tctttaaaat cactcatatc
     4801 tctaataacc tctcattctc ttatatttat attcctttta ttttcgtgat aaattataaa
     4861 tgaagtctag ttttgaaaat aaaatacttt tttaaatata ttattgcaaa taagtaaata
     4921 tatatatact ctcttatttt ttaatatatt taataatttc actcagaata tatatattct
     4981 ttactcaaat taaatataac ttatatactt tatgtgtaaa ttatggtaaa aatattattt
     5041 aatcaaatca aaacttgatt tatatacctg gaaaaggtat tatattaaag agtgcttaaa
     5101 atacgcattt cataaatagc tatatataaa ataataaata aggagagtga tgggcaatgg
     5161 atactagtcc gttgcggaga gaatttttta cattttcatc acttaataat atctaaaatc
     5221 aaaactaata catatattca gttatataaa ttagttaatt aatagatact attaaatgat
     5281 gagatttata taaaactgaa taaaggatgt gttgccatga taagatatta caaaactata
     5341 gattcaaaat tagagaaact tagttttttt gaagatggct gttggataaa tcttgttgaa
     5401 cctaatcata gtgaaatcaa cgaaatttct aatttattaa atatagatgt agaaagcata
     5461 gaatctgcac tagatgaaga agaacgctca agaatagatg ttgaagacaa tcatactctt
     5521 atacttatag atataccagt agacgaaagt gactctaatt cttctcatta tactacaata
     5581 cccttaggta ttatactaac tgaagaagct attgtaacag tttgtgatgc acaaactaag
     5641 attttaaatg actttatagt tggtcatatt aaagattttt ttacgtttaa aaagactcgc
     5701 tttttgcttc aaattcttca caaaaatgca gcttattact tgcattactt aagaaaaata
     5761 aataaaatga caatcataat agagcgagaa atttacaaat caatgaaaaa caaagaattg
     5821 gttcaattat tggaattaga aaaatctttg gtatacttct ctacctcact aaaatctaat
     5881 gaacttgttt taaacaaaat ggttagaact gctggtataa agaagtatcc agatgatgaa
     5941 gacctattag aagatgttat agttgaaaat agacaggctc ttgatatggc taaaatatat
     6001 ggagatatat tgagtagaat tatggatgct ttcagtgcaa ttataagtaa taatcagaat
     6061 aatgttatgc aaattttaac agtagttaca ctaattttct caatacctac aataatatca
     6121 ggattctttg gtatgaatgt catcaacatg ccattttcta ataacccaaa cggattttgg
     6181 ataatacttc ttattacctc tataatatgt atagtgatta catttttcat gtcaagaaat
     6241 aagctattat aaaatattaa taaaaaggcc attagaatta attgatttac atttatccta
     6301 atggctttta tatatttaaa tctatataat cttataatac tttttaacta tcatgttctt
     6361 catctataat catttcagtt tcaagccctt ctagcattga atctaaactt tcttttatat
     6421 ttagtatata aattggtaca tactcttctt catctattac ctcttcttgc aacttaaatg
     6481 gaggaattaa tacttcatca aagtctatat ttaaatttgg aaagccttct actatctctt
     6541 cacaattatc gtactcttca tttgctaaag aaatgtacat atctaggctc tttttataat
     6601 aaagacctgc aagcatatac tcacacttct tacttccata tttaatacct tctttataat
     6661 atttctcagc ttctgcgtca tcatttaatt ttaaataaat ttgacctaaa ttatatattg
     6721 attcaacaca accttgtgct ctagcctttt cataccaaaa tttagaattt acataatctt
     6781 tatagaacat atcataaatt aatcctacca tatgttgagc atacacatta ttttcattgg
     6841 caggtgtatc aaacatcatt ttagactctt ccaatttacc ttctctaaaa taaattctac
     6901 ctagatgtat acgagcattc atatggtttt tgctacttgc catttcaaaa tactttctag
     6961 cattttttgt atttcctaag ctctcatata tacaccccaa tctataacta gacctcgtat
     7021 ctccattttc agatgctttc ttataccaac ttattgcttc ttcaatatta tttaaatctt
     7081 catagatatt tcctaatttg atttgacaag atacattatt tggaatcctc gaataatacg
     7141 aaatcgcttt ttctatatct tgattctgat aatataaatc ccctagattt tcaagggaac
     7201 ttttgcatcc aacagctata gcatcctcat aatatttaat agcattttca taatcttttt
     7261 cttcaaaata tatacctgcc agattatttt ttgcttctgt tatatcatct tcagcagcca
     7321 ttttataata agtttttgcc ttctctatct cattttctct attatataaa ttagcaagtc
     7381 tatacttaga aagtatatga tttttttcta ttgctttctc ataataataa attgcatctc
     7441 ccagctcttc ttttctatca tatacatatg caagcctata ctcagacttc acatcatctc
     7501 tatcagcact ctttttataa tacttctctg catcttctaa attatcattt atttccataa
     7561 tcatacccaa gttaaagcaa cctttcatat gacctgactt gtacaactgt ttatataaac
     7621 ttactgttga tttattatct ttcttttttc catttaatat tgctaaatta tattttgcat
     7681 catcatctaa aagcacagaa ccttttttat agtattcata tgcttcatca tctttctcca
     7741 aaaaagtata taatacccct aaattaaaac aagctttacc taaattcttg tctgctgctg
     7801 tttttaacca tttttcacac cttacaaaat ctttcatttt aaaatggcat acacctaaat
     7861 tattttgtgc ttgtaaataa cctaaatttg ctatcttttc ataccaatat atagcatatt
     7921 catagccatc taacttatag tacatattac ctaaatcaaa agcagcatct tcacttccac
     7981 ttttatatgc catcgaatac cacttttctg cttctttaaa ttgtttttta ctttcatata
     8041 tatttcctaa agtatattga gattttacgt aattatccaa tgcagcactc ttatacaact
     8101 ctattgcgct ttccatctta cccatctttt caaggagggc acccttagta tgtacgactt
     8161 tcgggtcatt agatatattt tttatttttt ttatagaaaa ttttgtattt ttcattattc
     8221 gctacctacc cattttataa aatatattct ctcaaacatt attatacatt ataatctaca
     8281 attaatgcta taatattgaa tttttacctt ttgtcactat attgtaacat taaattttaa
     8341 attttataat cattagattt tattatgtaa ttggtatata atgtatatat aggggggaca
     8401 ttttatgaat ttcagcaata tttttatcca agttgcagtt ttatttataa ttattttagt
     8461 tggatatttt gtcagaaaat tcaacttatt agatgaccac tgcacatcta agctttcaac
     8521 tttgacaatg acagtttttt taccatcaat gataattagc tccatgcaaa ttaactttga
     8581 tagtaaaatg atacaaaaaa tactattgtt attatttaca tctttgatta tgtatatagt
     8641 ttcaattata attgcatttt tattgaaata cattttaaaa tgtaatgata aaaaagattt
     8701 gggaatatac cagtatatag ttgttttttc aaatgttgct tttatgggtt atcctgtaat
     8761 tgaagcagtt ttaggtcatg aagcaatatt ctatactgca atattcaatt tgccttttaa
     8821 cttattttct ttcactttag gaatttatct tttatctaaa ggcagtacaa gtagaggttt
     8881 ttcaataaaa tcacttataa gccctgctac aatagctatt gttattggac tttttttatt
     8941 tgtaacagga cttaggttac cccaatttat aaacgagcca cttgagatgt tgggaagtat
     9001 aactacacca atatccatga ttatcattgg aagcttactt gctaattctt cagctctcga
     9061 ttgttttgtc aacaaaaaat tatatatagt cacttttata agattattgg tacttcctgt
     9121 gattgtgtat tttattctaa aaggatggat aaatgataag atgattttag ctataccagt
     9181 agtaatttct tcaatgcctg ctgctgcaaa tactgctata atggctaatc aatatgactc
     9241 caatataaca ctagcttcac aatgtgtgtt ttttacaaca ctattttcag ttatatctat
     9301 accttttata agtattttac tcttatcttg atttattttt ttatgtatta tataataaca
     9361 tattcaataa attacaaata taaacatttt attgtagtat attgatggaa ttttttccta
     9421 gatatttgtc aataatatct atatgtatta tagaatagga ggcttaaata tggatatgga
     9481 tttactcttt aaaatagtat ttgctatagt tgctataggt gtttctgtaa aaatcattaa
     9541 aactgttgct agtttagctt ttaaaatagc gttgatattg ctaataattt tatttgtata
     9601 tagaatgttt ttgtaaacat ttaaaaataa gttttttaac acaatagaga agtaaaatag
     9661 attatcttta atgtaaattt tttattaaag acactctatt ttacttctct attagcttct
     9721 ttaaattaaa tttttattat atcttcccat aatataaaca tcttcaaatt ttccatctct
     9781 gattacagca tattttttag ttccctctat ttcaaaacct agctttttat ataaatttaa
     9841 tcccttttca ttatccacaa ttacttctaa tcctactcta gttaacataa tccaattatc
     9901 tgctatatct aaaagttgtt ccataagctt cttacctact cctcttccct gataattctc
     9961 tgcaacactt attcctattt ctgcgctatg tctcaatcgt ggagaagaat ttacatgtaa
    10021 cccagcaaaa cctattacat ctttttctcc attattatta gtttcagcta caaacatata
    10081 atcattttct cccaaagaag ctaacatagc ttcagtttta tcgactctgt cactcattaa
    10141 tgccaaagta tttctcataa cactgtatgt tcttctaatt tcattaaaag cttgtgcatc
    10201 ttctcttctt ataggtctta ttgttatatc tataggctta ctcatattgg attccccctc
    10261 aaacataatc tttattttta attaaaattt tacatcttta ttttatactg ttcaaatcta
    10321 tgtatgcttc tacctgaggt acatatatca tattatcttt tatatgtgta cttccaaatt
    10381 cttttaattc tgtaccttct ttatccttag ttattttata tctaatggat tccatacctt
    10441 caactacttc agcatgaaaa aacatacctt catattgttt aaatctaaaa ttaaaattgt
    10501 ctttagtaag ttttattatc atatttttat ctactcctta tataattgat atttttctta
    10561 ttattagttg tatatatact gcttattcat attaatatat tataacttat catttattta
    10621 gtataaagtt ataaatagat atatttatac ttattttaca atctttcttc aattgatata
    10681 taatatgttt atataaacta atgtatgtga ggtgaaatat gagcaaaaaa acaaaatatt
    10741 tttctttaat tctctttcct attgctttgc ttttaaattt tatagccagt aaagttccaa
    10801 atattgttga aaaatattac tcccaattca tagataaaat aattgtacaa atattgagta
    10861 aagtttctgg aattttccca ttttctctct atgagattac aatgtacata atagttatat
    10921 ctaccttctt atttttatgt tgtactttat ctacaatttt taataagaaa aaaaaattaa
    10981 acatatattt aaaaaattcc attttaaata tattatccat tgtatcaata gtctactttc
    11041 tttttgtagt gctttgggga ataaactata ataggattcc actagaaacc acacttataa
    11101 ataactataa tttaaaaaac aatacctcta tccaatcaaa acaatatagt gttaaagagc
    11161 ttactaaatt atataaattc cttgtaatta aagcaaatga aactagaaaa ctcacattac
    11221 aagataaaaa tggtattgta aaatctaata cagattataa tggtgtcata aatagagctc
    11281 aactaggata cgataatatc ttggatatat tacctggtgt tagtgatagt tattctaagc
    11341 ctaagtatat tatttcatca aatctaatgt gctatacagg aataactggt atatactttc
    11401 cttttacagg tgaggcaaat gtaaatatag caatacctga cttatatatt ccttgtacag
    11461 ttgaacatga aatggcacat caaaggggat ttgctagtga agatgaagct aattttatag
    11521 cgtatcttac ttctataaaa caccccaata ttgattttaa ctattctgga tatattttag
    11581 ctttaaatta tactgcttct gctttaagta aggttgatta taatgcatat gttgatattt
    11641 ctgctggcat ttcagattct gtccgtagag atttaaaaaa tgaaagtgaa ttttggcaaa
    11701 aatatgaagg taaaattaat gaaatttcta atgagtttaa caattcttac ttaaaagcta
    11761 atggcgttac tgaaggtact cagagttatg gtaaaatggt tgatttattg ctaacttatt
    11821 atgaattata tccatataac taaacactac aattcaaaat tttattcata tacagctgat
    11881 attaaatcag ctgttttcta tttaaataaa ttatttaact tcaagcaatc ttatataatt
    11941 tgatagtact tatactatct gtataacatt tcaattctaa ctattttagt taaaacattt
    12001 ctattatctt ttttaataaa tgttttaact aaaattttta aaacataaaa cttccacatt
    12061 atttcactat ctatataaaa atctaaaaat tcacatataa aattacataa ctaaaaaatc
    12121 ataaatttat agaaaacata tattcaaaat aaaaaagcat gtatcaaaac ttgacacatg
    12181 cttataatct tatgtatata tatcattttg tcacaataaa aatttacttg taacactgat
    12241 atttttaaac aaaatagtcc ttatttaatt aaattaacat taattaaatt cgtactcatt
    12301 ttgctatcat taaacattac ttatagtaat tttctaatac actatttgaa acttccttcc
    12361 cttgaaaact tttcaaccaa ttctcatatg ctgagttttt ccatacacta ccatcattaa
    12421 atgttacttg ttttacacaa gatataatgt actttacacc atgctctcca gttgattgtt
    12481 tccatttatc aaacaataga aaggcatcat tttgattctt acctggtgct attgcatttt
    12541 gctttgcata ttctttctcc atttgtttcc attctttcaa ccaattttct acccatgcct
    12601 tgccttgttg tttttcaaac atacttatta tatcctgtgg tggtgattgt tgcatttttc
    12661 tataagtatg tgaatatgat tttggagaca caggagatat gcctgtaaca ataccatagt
    12721 ctacaccagc tgggctaaat ccaacacttc ctacttcgcc atctgctgcc acattttgag
    12781 catcccaata tagctcaagt ggttttccat ttttatcata tgctaagcat gtaatttcat
    12841 aatccataat tgccttttca ctgctatttg ttaagcttat aacattgcta ggccaatgat
    12901 atggagtatc atcatactgt attttgctat tatttataaa taatggaata ttctcattat
    12961 aaacaagacc tttactactt tgaatatctg ataccttctt tgtgacagta cttaccgtat
    13021 ttgctaagta tacatttgtt ttttctgaat tggtcgtact aagagaaaat gttgttgtcg
    13081 ttccagtaat cagcaaagca acaactaaaa taggtaagct agatattttt ttatatctca
    13141 caattcttca cctctttttc tattaactta taataaaaca atttaccaaa acattaaaat
    13201 ctatgttaac ataatttacc aactgttcta gtaactatat cctatcatag ttactatcaa
    13261 gtttcaatac tattgatgga aattttcact atattaaatc tttttttaat tttataaatt
    13321 tacaatatga tttaactcat acatatatag tcaaattaga atataagttt cttacattta
    13381 atgaagttta taaaataaaa aatgactaga taattaaata ctgtaaataa aaacttatta
    13441 caaaagtatt taaaaatcta gtcatttaat atatatatga attgtatact ataatttttc
    13501 aatctcttct agcaactcat caactaattt gttaccatca atcattctaa ctatttcacc
    13561 ctttttaaat aaaagacctt cacctttacc cccagcaata cctatatctg cttctctggc
    13621 ttcaccaggt ccatttacag cacagcccat aattgcaaca gtgatatttt tttccatact
    13681 accaatcttt tcttcaacct catttacaac actaattaag tctatattac atctaccaca
    13741 tgtagggcat gatataactt taatcttatc atttaaaagt cccaaacttc ttaaaatctg
    13801 tttaccaaca ataacctctt caacagggtc gcctgttaaa gatattctaa cagtatctcc
    13861 aattccttta agaagaagtg ctcctacccc tatagaagac tttattgttc ctttgtgaac
    13921 acttccagat tctgtaattc caatatgaag aggataatca actttttttg atattaattc
    13981 atatgcatca acagttttat aaatatcgga tgattttaga gatataacta tattatgaaa
    14041 atctaagtct tcaagaatct taatatgtcc cattgcactt tcaactaatg cttcagcagt
    14101 tgcagagcca tactttttta aaagttcttt ctctaaagaa cctccattaa ctccaattct
    14161 aatttttaaa tttctttcct tgcatttctc aactaccatc ttaactctct ctatacttcc
    14221 aatattacca ggatttattc ttattccatc cacaccttgt tctatagctt caagggctag
    14281 cttataatcg aaatgtatat ctgcaataat tggtatattt acactagact ttatctttgc
    14341 tatattttta gcagctttaa tatcaggaac agcaactcta actatatcac aaccagcttc
    14401 ctctaatctc tttatttgag ctattgtagc atctgcatct cttgtatcag tatttgtcat
    14461 agattgtata cttataggat tttctcctcc tatttttaca gctcctacac ttacttctct
    14521 cgtctttctt ctctcgtaac tcatattcca cctctatata ttattttata ttgaatctct
    14581 ttaaatatta aaatattata acctacatgt atattataaa ttaaaaagct atctagttaa
    14641 ataagaataa atttcatata taaaaattaa tttattctta tttaaaacta catagcctat
    14701 tttcttaaaa tctctaaaaa actaaatcat tgaaataatc ttagtatatc tttatatgtg
    14761 ataaaaagca taaataacat taaagctcca aaaccaacaa catttatcat gccttcttta
    14821 tttgggtcga gttttttacc acctctaaca gcttctagta acaacataag tattctccat
    14881 ccatcaagtg ctggtattgg taatagattt actataccta aatttaagct tatgacagct
    14941 gctaaataca ctacatttat gatgcctgta cgagcagcat ctgataccat accaataaca
    15001 cctactggtc ctgctacagc attgccaatc cctcctggta cacgtcctgt aatcatttga
    15061 cctagaaatg ttagcatctg ttttgtcata tctacagtag ttttacatgc atttactatt
    15121 gaccctaaaa agtctttttc tctttgtggt tgaattccta tttcatactt tccattgtta
    15181 tttttaggtg ttacatttac aactttatca cttccatctc tagttatact aagttttaat
    15241 tctccaccag aagaagtgtt tattatattt gcaacttcat cccaagtctt aacagaatta
    15301 ccattaattt tatttatctt atctcctact tgaagcccta cagcctgtgc aggagtatct
    15361 ggcattatct ttcctaattt agtcgttgga gaacctatat acaaaaatac tggaactaaa
    15421 agtattgctg ctaatattat attgaatatt ggtcctgcta caatagtact aaatctttgt
    15481 aatatagact tgtttccaaa agaatttgga cttatttgtt cttcatcttc accttccatg
    15541 ctgacatatc caccaattgg aagtgctctt atagaatact ctgtatcttt ttttacacta
    15601 tatatcttag gccccatacc tatagaaaac tcatttactt tgataccact tctttttgca
    15661 aatataaaat gtcctaactc atgtattaat acaattatac taaatagtat taatgcggct
    15721 attatagtca taaattcacc ttcctatttt tttatcagat ttgcaacata agctctactc
    15781 catctatctg tttctaagat ttcttctaat gttggttcac ttatgctact atgaacttct
    15841 aaagtcttct ctatgtaata tggtatgtca tagaaaccaa ttttatcctc taaaaattca
    15901 cttactaaaa cttcatttgc tgaatttaaa acagaagaat aagttcctcc catttttaaa
    15961 cactcatatg ccaattttaa acatggaaaa gtttctaaat ctggttcttc aaaagttaaa
    16021 gtactaaatt ttgaaaaatt cattctttca aagctacttt ccattctatc tggatatgta
    16081 agagcatact gtattggtag tctcatatct ggacatccta attgagcaat tattgagcta
    16141 tctgtatact gcaccattga atgaattatg ctttgtgggt gaactactac atctatattt
    16201 tcttgttcta ctccaaataa ccatctagct tctatcactt caagtccttt attcataagc
    16261 gttgaagaat caatacttat ttttcttccc atactccaat ttggatgctt caaagcttca
    16321 tttttagtta tatttgcaag ttctcccttt ttctttcctc taaatggacc accagatgct
    16381 gtaagtatta tcttttctat atttttttta ttttctccat ttagacattg aaatatagca
    16441 ctatgttcac tatcaacagg aagaatattt acattgtatt tttctgcttc tttcattaca
    16501 agttcaccag cagttaccaa cgtttcttta ttcgctaatg ctatgtctat tcccttcttt
    16561 atagcacaaa gagttgggac taatcctatc atacctacta cagcagttaa aaggacatcc
    16621 acttcatcaa gtgatgatat tatttttaat ccttccattc ctgctaaaac ttctatttct
    16681 atatttttag gcaacatttc ttttaatttt aatgctttat tttcctcaaa aactgttaca
    16741 tatttaggtt taaactctac tatctgttct agcaataatt caatattact gttagctgat
    16801 attgcaacta tctcaaactt atctctatgt tctctaacca catccaaagt ttgtttacct
    16861 atagagcctg tagaacctaa aattgatatc tttttcatat aataatcctt tctaataata
    16921 aatataccct gtcaaaacat tataacacta ctaaaacaat tatttttact attcatagaa
    16981 atttcatata ttattccaca gattaaaatt aatctattat tttttgtatt ttgtggaata
    17041 ttataaaata ttctagtatt attaaatgcc cttttttatt tccacaacat cttctatttt
    17101 acagtctagt gctaaacata tcttctccat tgtatccata gaaacagatt tatcatttcc
    17161 tagtctgcct aaaacagaaa tacttatacc tgatatattt acaaattcca ttttctttat
    17221 ttttctgtca attaacaatt tccataatgg agcgtatgaa atcatgttat cacctcgcaa
    17281 aatatattct atcacatttt atctattttg gaaaattttc atctatttag ataaataatc
    17341 acctgcttaa gtgtcaaata attttatcct tctaatatga aaatttaata ctatactaaa
    17401 tgattggtat ataacacata tgatgcatta ttattaacat ataaaaattt ttattggtgc
    17461 taaattaaag taatatacta ttattatatt tgcagatata taagaaagat tttattatat
    17521 atttaaagta cttgattata ataagagaca agggctatga tggatactat agaaagttag
    17581 aactatacta ttttagaatg aacaattaaa ataattaaaa ttagtttaat cttttattct
    17641 tgataaaatc atattaaata aatacaaaa
//
LOCUS       DAMJVK010000083        17548 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.084, whole genome shotgun sequence.
ACCESSION   DAMJVK010000083 DAMJVK010000000
VERSION     DAMJVK010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17548
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.084"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            128..874
                     /locus_tag="PC414_002816"
     CDS             128..874
                     /locus_tag="PC414_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HDF2465201.1"
                     /translation="MKIENLSIDDIKRGYIFNDDLNCYSCIKCTKTYEVGEIYKIGNR
                     FFEASKAIELHMELEHSDYLEQLLNSDSKYNTLTENQKQLFQLFISDLPDKEIAKQVG
                     VSTSTIRHQKFIFREKAKQAKLYLALYESVFEDKSNKSSTIIPIHEHARMVDERYVVT
                     EEERKHILDTSFESMNPLKLKVFSSKEKKKVVILSKILEQFERGKKYSEKEVNQILES
                     IYDDFATIRRYLIEYGFMSRNKECTKYWLT"
     gene            935..1609
                     /locus_tag="PC414_002817"
     CDS             935..1609
                     /locus_tag="PC414_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HDF2465202.1"
                     /translation="MNDELKSILYNKGVDIVRFVDISEFPINQTQGFSKAILFCIGLS
                     KKFITDIYNNLPTDSDEFLEKEEKAEELADWISEYIQNKGYRAYSQSEKNNLEHGYFE
                     KAYINPEMQSGISPLPHKTIANISGIGFMGKNNLFVTEEYGCAFSMCTVLTDAPISVE
                     RYPLIDSKCMDCNVCVENCPAKAIHGNEWTLPGKRESIIDVSKCFCVLKCMMSCPWSL
                     RYANQK"
     gene            1977..3323
                     /locus_tag="PC414_002818"
     CDS             1977..3323
                     /locus_tag="PC414_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HDF2465203.1"
                     /translation="MEIETKTPDWINVLELNSKIDITGKYDVSNIIQDIVSDKSLEGS
                     IIYFPKGNYLFEKGIKISQQITLQGDSYYGGDNQVSNLDKKQSIIGTTNFITRGVSNM
                     SIIALTGTSQCIKNINFYYDSHDIEKIPPKNVSAITEYGETQGLSHFEHLFISGFSGI
                     GIEIPYYSTGNDIIVSSCGLGMRLGEKSMLSSSKIYECKNGMEITTGVSLNNVRIEEI
                     KEIGINNKGFGFNLIMNLMVDQCGYCGFMFEKMCYSQITARITRCGQYYKSVDYNAYE
                     NMEDRKEKAYSIFYGDILESCNIVLMNRNIDTLESDSLNEHKIYVIKANETNNVMLTC
                     YAEADNFIQCAKGNLLLENGENTYKFYNGEICSVGGVQISDIDEKDLIKIIENTLYID
                     DKGKYLMVPRLKKNTVIYSVLDSTEEINKLHGGIWEQIDVKVLAGESMYYYKKIKD"
     gene            3350..4750
                     /locus_tag="PC414_002819"
     CDS             3350..4750
                     /locus_tag="PC414_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HDF2465204.1"
                     /translation="MEESIIIKDISGWINVLDYGICNDETKDVSFKLQEIMDAVPEDS
                     IIYFPKGKYLFESGVKIKKRLTLCGDSYILANVPKNTAGVTQFTFKGKKNPKSENRAI
                     ITAKSVRHCIKNICFYSDSCDRDMTSNHEPPTDGKPRFHHNVIINYRNISAVICEDKQ
                     GEPGHYENLSFSGFSGIALDMPNNSTANDITIFTSGLGISTGENSTIANSKVWGCVDG
                     MIISTGTFINGMRVEEIKKVGIKNIGRGLNFITNITIDQCGYCGVWFDSISNVQISGN
                     ITRCGQYYYDVSYDNYLMLDNRVKEAYSLFYGNLLESSNIVLSNTNEDNWEDFKEDKH
                     KVYLIEALKTKNVLLTCNIDATDWIVKSEKGNLTYNNMRQTVIFYDGKLSSINGIGIS
                     DEDYGDKVKIKKGNIYINSNDKDIKMHKPEVGDIMSTIIKDKSILSAHYGGTWEKIGE
                     KIEFGITVHYYKLIEK"
     gene            4960..6396
                     /locus_tag="PC414_002820"
     CDS             4960..6396
                     /locus_tag="PC414_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HDF2465205.1"
                     /translation="MEGRTGIKDGRWINVVERGIKNDGSYNIGPALQDIIDEIETDFC
                     VLYFPKGKYLFTSGVKINKEIVLQGDSNSSTGTTQFITRGVENMSIITLTGKKQCIKN
                     INFYSDSCEIQVNKEPPTKGNPKYHYEMIINKNEKNVEMNNVSAILYDNSKNMIKGLG
                     HYENIYISGFSGTGIRIPYYSIVNDITVSSCGLGIDTGIDTIISNSRISKCQNGMRIR
                     TGTSINNVRIEKIQKVGLISSQVKGYEGYGSYKINNITIDQCGYCGFSFDSMKDSYFS
                     GIIRRCGQYYYNTDYDTYLSIKDRVEEAYSLFYGNYFTECNIDLVSDNKDNWDDGLEY
                     KHKVYVFKTLQIPKLTLRCNIDAYDYIVKSVHGGGNLMLKNNFNNYIFIDSSNPEVIN
                     GINFNYQKNGNLLRLNNGKLSYKNGKEETAIFRPNYGLIVASTDSECEQIEATYGEKW
                     EKLGSEVIGEETVYYYKHHEPDKTNQIQ"
     gene            6740..7684
                     /locus_tag="PC414_002821"
     CDS             6740..7684
                     /locus_tag="PC414_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HDF2465206.1"
                     /translation="MIVSVSRRTDIPAFYSKWFFNRLEEGFVYVINPMNPKQVSKIEL
                     NPHTVDCFVFWTKDVTPMLYDLNKLRDYKYYFHYTITSYGKEVETCILDKRKVIDSFK
                     ELSRKIGKERVILRYDPIFLSEKYTVDYHIKAFASLCNQLDGFTEKCIISFIDLYKKT
                     KFNTKALHIKPIEITEIETLSKEISKIAHKHGIILEMCSEEYNLSKFGIGKSKCIDDR
                     LISKIIGCEVEVKKDSTQRDICGCVKSVDIGQYNTCRHYCLYCYANFNYAKVEENCRL
                     YKEDNKLLIDTLRDDAKISIRDMKTIKVDKNKQISIFD"
     gene            7853..8785
                     /locus_tag="PC414_002822"
     CDS             7853..8785
                     /locus_tag="PC414_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HDF2465207.1"
                     /translation="MNLLKKYPIPIASLILSIFTLGNLLQSYSESLRNVIGFIGFILY
                     AIYIVKIILLRKGVKEQLENPLISSTFPTITMATMIFATYIKPLSLELSIMIWSIGIV
                     GHLVLIVLFSKKFLVKFSIKTVFPSWYIVYVGIVAASVTAPAFNQLLIGKIAFWIGFV
                     SYIVLIPVILKRVWIIKEMPEPSLPSIAILAAPGSLLLSGYINSFSDKNNVVLYLLFI
                     LSIVFYIIVLGYLPKLLRLPFYPSFAAFTFPMAISAMGMKLMNGYLTKSGHFISWISY
                     ASKLEEIIATVIILYVLVLFIIYLSKSETTSKAK"
     gene            8890..9357
                     /locus_tag="PC414_002823"
     CDS             8890..9357
                     /locus_tag="PC414_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C_GCAxxG_C_C family protein"
                     /protein_id="HDF2465208.1"
                     /translation="MKTKEEYLKEVRNISEEYFRKGEFFCSEAVLQTINDALGQPLSP
                     EITKLASGFPIGLGKAQCLCGAISGGEMALGIVYGRVHGEAMNPKMFEHAKGLHDYIK
                     KEYGATCCRAITKKWDGDNFMSPERKEHCIKITGQVTEWVADKLIEDEKLNIK"
     gene            complement(9601..10455)
                     /locus_tag="PC414_002824"
     CDS             complement(9601..10455)
                     /locus_tag="PC414_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009912760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HDF2465209.1"
                     /translation="MLTIIIALLPVMGWGLMPIVANLKKSSPHEQLLGTSISAFIFST
                     IITLVIHPEITFFSFCISMISGIFWSLGQLMQFKAIQIASVSKVMPISNGSQLTFTTL
                     LAVILFNEWTSSKMLLIGSLSILCIILGILLTNYQTKKSTDKNMLKCVGVVLLSSLFL
                     SLYVVTNQIFLIEGYRIILPQSVGMLITSSIIVKYFSKEIVYRENVLFNLITGILWSI
                     ANLGMFITTNTLGVTISFSISQSCVIVATLGGIIFFKEKKNKKEWLAIFIGIILIMSG
                     VFMLSIIK"
     gene            10621..12585
                     /locus_tag="PC414_002825"
     CDS             10621..12585
                     /locus_tag="PC414_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2465210.1"
                     /translation="MLTDMIQNREIKIIKFLLKEGHTTVKAIADDLDVSEKTVSKSLK
                     EVEAVLKKIGLSLIRKPKIGVYIDGDLKKILPYLDNKGSQIPTTREERVIYIYSVLLK
                     SNDYITIQQLSDELYISRGTIERDLAEVEKLLKNEGISIYKKPSKGIKLNISERDKRQ
                     LTAKFIHKFWSRNWYIKQEQESFYQAFEKIQADVNGIFSEDDLSIIIDILRNFSRERK
                     FEFSDYEFQSLVIHLAIAVERIRKGEYIEESLYTGNIENNQMSNTNYLAKQLEEKFSI
                     LLPKSEIGYINLHLVASDQHRDTNELKNTSTNPCETSTNNISNLREIIKNTLYETAYD
                     KDLIDGLTSHVHSSINRLKYGLSIKNPYVNTIKQNFSLSFEQALDLKKVIEFIYDIRI
                     NDDECAYIALHFEAFRERMKELPQNIRVILVCSTGLGSSRLLSARIKKYFPMIKIEKV
                     LSVQELVSSKIDADLVISTIYLELETIPTIVVSPILNESDIRTLERNIQNVSSYTSKK
                     YKSFRNLIFEENILLNIEASNYEDAIKYITDNLIKKGFAKEGIAKSAIERENLSYTSF
                     QSIATPHANPSYIKKSNISVATLKNPITWGNEKVEVIFFISLENDKNLELDEIYEVFY
                     DFIDNKKNINDILSATSTKEVYDLLVEESI"
     gene            12585..13046
                     /locus_tag="PC414_002826"
     CDS             12585..13046
                     /locus_tag="PC414_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2465211.1"
                     /translation="MKISDVLKKEQIVFNLDTNTKEETIKKLSSIFVENGIVDNEEEY
                     VKSVLEREEHSTTGIGNGIAIPHGKSNAVKKSSIIFAKTKHKIEWDALDEKPVDSIFL
                     LAISNIDKDSNHLVLLSKLATKLMDDDNVDALKLANSEQEIINIFSEEGEM"
     gene            13049..13369
                     /locus_tag="PC414_002827"
     CDS             13049..13369
                     /locus_tag="PC414_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HDF2465212.1"
                     /translation="MKRKIIAVTACATGVAHTYMAAQALKKASKKMGHLIKVETQGAT
                     GIDNELTTKDAQIAEVVIFAVDTKVRNAERFEGKEILEVPVNAPIKDAEKVINDALKL
                     IDTK"
     gene            13385..14485
                     /locus_tag="PC414_002828"
     CDS             13385..14485
                     /locus_tag="PC414_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDF2465213.1"
                     /translation="MLKKIVSELKKHVLTGISYMIPLVIAGAMIMAISRVGGSFYNIP
                     DIWDAKYAESASSIVRLLHDLDGFGGTALGLMFPVIAAFIGFSIADKLAIVPGLVGGM
                     IAKDIGAGFLGALAVGLIAGYTCLFIKNHVKLPKSAASIVPIFIVPVFGTLITVVLIN
                     YVIGIPFATLNTGLENWLNGLSGTNQILMAAIIGAMMAVDLGGPVNKAALTTSLALLT
                     SGIYAPNTAAMVGIVIPPLGLGLATILAKQKYNKQLREAGKSSLIMGLIGVSEGAIPF
                     AVESPLKVIPSCVIGTAIASAMAVGLGSVNATPISGFYGWFTVENWPMYVLSIAVGTV
                     IVAGLVIVLRGNPVIEDDEFEDDDFDEAEWES"
     gene            14605..17205
                     /locus_tag="PC414_002829"
     CDS             14605..17205
                     /locus_tag="PC414_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HDF2465214.1"
                     /translation="MIKAHIVNHTHWDREWYFTSGDALVLSEQLFTDVIDELERNPDV
                     SFVLDGQLSILDDYVQLHKEKIEIIKKLIKEDRLHIGPWFTQTDAFFARGESILRNLM
                     IGIFESKKYGKYMKIGYLPDTFGFNAQMPILLKHVGLDNIFFWRGIHLGKQVQSPYFK
                     WKSLNGESYIYAVNMPHGYGTGMLLEPSLKYVNGRLDPAIDFIKSYTDVDEVLIPSGN
                     DQLNIIGDFKNKIKAINDIGKYSYITSSYQEFLKYIKSIESLESYRGEFREPVLARIH
                     KSIGSSRMDIKLACDKLENKLIKRIEPLLVIAKKSKIEISNQLLINTWKKLLEGQAHD
                     SLAGCVTDTVTDDILHRIREANEICDSIENTIVKKISEGLKLSKNDILVFNTEAKRFN
                     GYKEIQVVSDSKNIYFKDNLDATIIEETYVKPRENVLEETPAGNIFIEEPGYYILKVR
                     INVQLPALGYKVVSFESSDKEMVSLNKSNDAFISNDNYKIIYEDGSLNLQLKDGTYIS
                     NFLTLKDSGNAGDTYDFSPLKNDEDIKLSFDKVYTEKALGYEKMVVKGTTLLPLTLED
                     RKNKYLNGKLNVKVSIVLSKENPLMDVKLCVDNTIYNHRLRVHIKTDIKDNKNIASLP
                     FGYITRENGVLDNWEDIYSEMPIDLEPLESNITLTNQNRSCTIFTKGIKEYQHIEDEI
                     ALTLLATTDELGKPDLLYRPGRASGDTTKKGHIRIKTDKAQMLKKLEFSFAIYMDSKS
                     FDELEIANLTHNYLRECVNYQLQDYNFFLYRIDNKIQKSIVNKTVSRELEIISLPENY
                     LISACYPSYYSKDKFIIRVENPSSNKMILDEQIFKNRNGKIVNAIEDVEEEQVYEIAP
                     FDVISILLDL"
BASE COUNT         6990 a         1793 c         3025 g         5740 t
ORIGIN      
        1 tattttgtaa tttcaaatta ataaataatt agatttgata aacaaaaaat aaaaatgttg
       61 attatattaa taaactttaa tataatatat ttatgaaaaa tgtaaactgt gaaatggagg
      121 tagtaacatg aaaattgaaa atctttctat tgatgatatt aaacgaggct atatatttaa
      181 tgatgattta aattgttata gttgcattaa atgtacaaaa acatatgagg taggagaaat
      241 atataaaata ggtaatagat tctttgaagc ttcaaaagct attgaacttc atatggaatt
      301 agaacatagt gattatttag agcaattgct taatagtgat tcaaaataca acacattgac
      361 tgaaaatcaa aaacaattat ttcaattatt tattagtgat ttaccagata aggagatagc
      421 aaaacaggtt ggagtatcca catctactat acgccatcaa aaatttattt tcagagaaaa
      481 agcaaaacag gctaagctct atttagcact atatgaatct gtatttgaag ataaatctaa
      541 taaaagtagt actataatac caatccatga acatgctcga atggtagatg aaagatatgt
      601 agtaactgaa gaagagagaa aacacatttt ggatacatca tttgaaagta tgaatccttt
      661 aaaattaaaa gtattttctt caaaagaaaa gaaaaaggtc gtaattttat ctaagatttt
      721 ggaacaattt gaacgtggga agaaatattc agaaaaggaa gttaatcaga ttttagaatc
      781 tatatatgat gattttgcaa caattagaag atatctaatt gagtatggtt ttatgagtag
      841 aaataaagaa tgtacaaaat attggttaac ataattaaaa taataaattt tactaatgtg
      901 taatgttatt tttatctagg gggaggagtc aaatatgaat gatgaattaa aatctatttt
      961 atacaataaa ggtgtggaca ttgttcgctt tgttgatata tctgaatttc caataaatca
     1021 aacgcaaggt ttttctaaag caattttatt ttgtataggg ttatcaaaaa aatttattac
     1081 agatatatac aataacttac ctacagatag tgatgagttt ttagaaaaag aggagaaagc
     1141 agaagaatta gctgactgga tttcagaata tattcaaaat aaaggatata gagcgtattc
     1201 tcaatcggag aaaaacaatc tagaacatgg atattttgaa aaagcctata ttaatccaga
     1261 aatgcaatca gggattagtc ctttaccaca taagacaata gcaaatatta gtggcattgg
     1321 atttatggga aaaaataatt tgtttgtaac agaggaatac ggatgtgctt ttagcatgtg
     1381 tacagtactt acagatgctc caatttctgt tgaaagatat cctttgattg attcaaaatg
     1441 tatggattgt aatgtttgtg tagaaaactg ccctgcaaaa gctatacatg gaaatgagtg
     1501 gactttgcca ggaaaaagag agtctattat tgatgtttca aagtgttttt gtgtattgaa
     1561 gtgtatgatg agttgcccat ggtcattaag atatgcaaat caaaaataat tttactagag
     1621 aaggtattaa attgaatatt ataaaatttt taaaatgtaa gattatataa gatttttcaa
     1681 aataaaaatt tgtatagaac actcagaaat gagtgttttt ttattggaat taaattatat
     1741 tgatacaaag tttaactatt ttaatcatta tataaaattt gtattaaaaa attaagaata
     1801 acttggtaat agtgaaaata taatgagatt ataattgatt tataatataa ctataatgat
     1861 gttgtaaaat gaaaattgtc atttcaaaat tcccaaaaag agtaaaagtc acttcggtta
     1921 tagtcaaaaa attttttcag tgaaaagaat ttagatttaa aagggaggaa ttaatcatgg
     1981 aaattgaaac taaaactccg gattggatta atgtactaga attaaacagt aaaatagata
     2041 ttactgggaa atatgatgta agtaatatta tacaagatat tgtctctgat aagtcactag
     2101 aaggttccat tatatatttt ccaaaaggta attatttatt tgagaaaggt attaaaatta
     2161 gtcaacaaat aacacttcaa ggagattctt attatggagg agataatcaa gtatcaaatt
     2221 tagataaaaa acaatcaatc ataggaacaa ccaattttat aactcgagga gtttctaata
     2281 tgagtattat tgcattgaca ggaacaagtc aatgtatcaa aaatattaat ttttattatg
     2341 atagtcatga tatagagaaa ataccgccta aaaatgtatc tgctattaca gaatatggag
     2401 aaacacaagg actatctcat tttgaacacc tgtttatttc tggattttca gggataggga
     2461 ttgaaattcc atactattca actggcaatg atataattgt tagttcatgt ggtcttggta
     2521 tgaggcttgg agagaagtca atgctttcta gtagcaagat ttacgaatgt aaaaatggaa
     2581 tggagattac tactggtgtt agcttaaata atgtgagaat agaagaaata aaagaaatag
     2641 gtattaataa taaaggattt ggttttaacc tgattatgaa tcttatggtc gaccaatgtg
     2701 gatattgtgg atttatgttt gaaaaaatgt gctatagcca gataactgcc agaattacta
     2761 gatgcggtca gtattataaa agtgttgatt ataatgctta tgagaatatg gaagatagaa
     2821 aagaaaaagc ttattccatt ttttatggag atattttaga aagttgcaat attgtattga
     2881 tgaatagaaa tattgatacc ttagaaagtg attcacttaa tgaacataag atatatgtaa
     2941 tcaaggcaaa tgaaacaaac aatgtaatgc taacatgtta tgctgaagca gataacttta
     3001 tacaatgtgc aaagggcaac ttgctgttag aaaatggtga aaatacttat aaattttata
     3061 atggtgagat ttgttctgta ggaggtgttc aaatttctga tatagatgaa aaagatttaa
     3121 tcaaaataat agagaacact ttgtatatag atgataaagg taagtattta atggttccta
     3181 gattaaaaaa gaatactgta atatattcag tactagattc tacagaagaa ataaataaat
     3241 tacatggtgg aatatgggaa cagattgatg taaaagtatt agctggggaa tccatgtact
     3301 attataaaaa gataaaagat taaattatca aactaggagg tagtgattaa tggaagagtc
     3361 tataataatt aaagatataa gtggctggat taatgtactt gattatggaa tttgtaatga
     3421 tgaaacaaaa gatgtaagtt ttaagttgca agagattatg gatgctgtac cagaagattc
     3481 tattatatat tttccaaaag gaaaatactt atttgaaagt ggtgttaaga ttaagaaacg
     3541 attaacctta tgtggagatt cttatatttt agcgaatgtt cctaaaaata ctgctggagt
     3601 aacacagttt acttttaaag gaaagaaaaa tcctaaaagt gaaaatagag caatcataac
     3661 agcaaaaagt gtaagacact gtattaagaa catttgcttt tattcagata gttgtgatag
     3721 agatatgaca tctaatcatg aacctccaac agatggaaaa cctagatttc atcataatgt
     3781 aattataaat tatagaaata tatctgctgt tatatgtgaa gataaacagg gagaaccagg
     3841 tcattatgaa aatctatctt ttagtggttt ttctggaatt gcacttgata tgcctaataa
     3901 ttctacagca aatgatatta caatttttac atccggactt ggaattagta caggggaaaa
     3961 ttcaacaatt gctaacagta aggtttgggg ctgtgttgat ggaatgataa tttctactgg
     4021 aacttttata aatggtatga gggtagaaga aataaaaaaa gttggaatta aaaatattgg
     4081 tagaggttta aattttatta caaatatcac aattgaccaa tgtggatatt gtggagtttg
     4141 gtttgattct atatccaatg ttcagatatc aggaaatatc acaaggtgtg ggcagtacta
     4201 ttatgatgta agctatgata attatttaat gttggataat agagttaaag aagcatattc
     4261 tttattctat ggtaatttat tggagtcaag taatatagtt ttatcaaata ccaatgaaga
     4321 taattgggaa gatttcaaag aagataagca taaggtgtat cttatagaag cacttaaaac
     4381 caagaatgta ctattaacat gcaatataga tgctactgac tggattgtta agtcagaaaa
     4441 aggaaattta acgtacaata atatgcgaca aactgttata ttttacgatg gaaaactaag
     4501 tagtatcaat ggaattggta tatctgatga agattatggg gataaagtca agataaaaaa
     4561 aggaaatata tatataaata gtaatgataa ggatattaag atgcataaac cagaggtagg
     4621 tgacattatg tcaacaatta ttaaagataa aagtattctt tctgctcatt atgggggaac
     4681 atgggagaaa attggtgaaa aaatagagtt tggaataact gtacactatt ataaattgat
     4741 agagaaataa gtaaatacgt agtatttgaa tagatgtatt ttattggaga gttaggacta
     4801 tagttttaat ataaatggtt tggtaatagc atggattgaa taatataaaa ataatataaa
     4861 aaattaattt gattatgtaa atttctatga agtagtaaaa ctgtgacttt agtcatagga
     4921 ggttcaaact aagtaaaagg gaaaggaata tataatatta tggaaggaag aactgggatt
     4981 aaagatggaa gatggattaa tgtagtagaa agaggtatta aaaatgatgg ttcgtacaac
     5041 ataggtcctg ctttgcagga tattattgat gaaatagaaa cagatttttg tgtcttatat
     5101 tttccaaaag gtaaatacct atttacaagt ggtgttaaaa ttaataaaga aattgtattg
     5161 caaggtgatt ctaattcatc tacaggtact acacaattta taactcgtgg agtagaaaat
     5221 atgagtatta ttacattgac agggaagaaa caatgtatta aaaatataaa tttctattct
     5281 gatagttgtg agatacaggt aaataaagaa ccaccgacaa aaggtaatcc taaatatcat
     5341 tatgagatga ttattaataa gaatgaaaag aatgttgaga tgaataatgt atcagcaatc
     5401 ctgtatgata acagtaagaa tatgattaaa ggtctaggtc attatgaaaa tatttatata
     5461 agtggttttt cgggtacagg aattaggata ccatattact caattgtaaa tgatattaca
     5521 gtttcatctt gtgggctggg tattgataca ggaatagaca caattatttc taatagtaga
     5581 atatctaagt gtcaaaatgg aatgaggatt agaactggaa ctagtataaa taatgtaagg
     5641 attgaaaaaa tacagaaagt tggtcttata agtagtcaag ttaaggggta tgaggggtat
     5701 ggaagttata aaattaataa tattacgatt gaccaatgtg ggtattgtgg cttttcattt
     5761 gattcaatga aagatagtta tttttctggt attataagaa ggtgtggtca gtattattat
     5821 aatacagatt atgacacata tttaagtatt aaagatagag tagaagaagc atattcttta
     5881 ttttatggaa attattttac agaatgtaat attgatttag ttagtgataa taaggataac
     5941 tgggatgatg gcttagaata taaacataaa gtttatgtat ttaaaaccct tcaaatacca
     6001 aaattaacat tgagatgcaa tatagatgcc tatgattata tcgttaaatc ggtacatgga
     6061 ggaggtaatt taatgttaaa aaataacttt aacaattata tatttattga ttcatctaat
     6121 ccagaagtta ttaatggaat taattttaat tatcaaaaga atggaaactt gttaaggtta
     6181 aataatggta aattaagtta taaaaatgga aaggaagaaa cagctatatt cagacctaat
     6241 tatggtctta ttgtagcatc aacagattca gaatgtgaac aaatagaggc tacctatgga
     6301 gaaaaatggg agaagcttgg ttctgaagta attggagaag aaacggtata ttattataaa
     6361 catcatgaac cagataaaac aaaccaaata caataaaatt tgtattatat aatgatataa
     6421 agttttaata tatagtgttt acctttaatg taatattggt atatttgtat agtattaaat
     6481 ttgttaaaaa gtaaaattgt agggtgcttg aaaatagcac cttttttagt ttcattataa
     6541 aattagtttt gaaagtttag gaataatgta tatttatttt taaagaatta taaccttatt
     6601 aaggatattt taaatgctag aaatgaatca gtaaatcata ctggaattat cttaaatgtt
     6661 ttgattaaat taagtattta gagctataat tatattaaaa atatattaga acataattat
     6721 attactatgg aggttaaata tgatagtaag tgtgagtaga agaactgata tacctgcttt
     6781 ctatagtaag tggtttttta acagacttga agaaggtttt gtttatgtaa ttaatccaat
     6841 gaatccaaaa caggttagta agatagagtt aaatccacat actgttgatt gttttgtatt
     6901 ttggacaaag gatgtaactc caatgttata tgatttaaat aaattaagag actataaata
     6961 ttattttcac tatactataa catcatatgg aaaagaagta gagacatgta ttttagataa
     7021 aagaaaggta atagattcat ttaaggaatt gtctagaaaa ataggcaaag aaagagtaat
     7081 tttaagatac gacccgattt tcttaagtga aaagtacaca gtagattatc atatcaaagc
     7141 atttgcgagt ttatgtaatc agcttgatgg atttacagaa aaatgtataa taagttttat
     7201 cgatttgtat aagaaaacaa aatttaatac taaagcatta catataaagc caatagaaat
     7261 tacagaaata gaaacattat caaaagagat ttctaagatt gctcataagc atgggataat
     7321 acttgaaatg tgttctgaag aatataattt aagtaaattt gggattggaa aaagtaagtg
     7381 tattgatgat agattaatat caaaaattat aggatgtgaa gtagaggtaa aaaaagatag
     7441 tacacaaaga gatatttgtg gatgtgttaa aagtgtcgat ataggacaat acaatacttg
     7501 cagacattat tgtctgtatt gttatgctaa ctttaattat gcaaaagtag aagaaaactg
     7561 taggctttat aaggaagata ataaattact catagatact ttaagagatg atgcaaaaat
     7621 aagcattaga gatatgaaga ctattaaagt agataagaat aaacaaatta gtatatttga
     7681 ttaaaataaa tatactaatt accttaatat attaagaata tagatacttt tgataacaaa
     7741 attaaactta atagatattt tctatataag aaagatggtc ataatatgtc aatttgacca
     7801 tctttttgat ataattaaaa cacaaataat ttagtaacaa aggaggcaga acttgaattt
     7861 attaaaaaaa tatcctatac ctattgcaag tttgatttta tcaattttta ctttaggtaa
     7921 cttattacaa tcttatagtg aatcattacg taatgtaatt ggatttatag gatttatttt
     7981 gtatgcaatt tatatagtca aaataatatt acttagaaag ggtgttaaag agcaattaga
     8041 gaacccttta atatcaagta catttccaac tataacaatg gcaacaatga tatttgctac
     8101 atatataaaa ccattatctt tagaattaag tattatgatt tggagcatag gaattgtggg
     8161 tcatcttgtg ttaatagtct tatttagtaa gaagttctta gttaaattct caataaaaac
     8221 ggtatttcca tcttggtata tagtatatgt tgggatagtt gcagctagtg ttacggcacc
     8281 agcatttaat caacttttga ttggcaaaat tgccttttgg ataggatttg tatcttatat
     8341 agttcttata ccagtaattt taaagagagt atggataatc aaggaaatgc ctgaaccatc
     8401 cttaccaagt atagctatat tagctgcacc agggtcacta ttattgtctg gatatattaa
     8461 ttctttttca gataaaaata atgtagtatt atacttgtta ttcatattat caattgtatt
     8521 ttatattata gttttgggat atttaccgaa attattaagg ttgccttttt atccgagttt
     8581 tgctgcattt acattcccta tggcaataag tgcaatgggt atgaaactaa tgaatggtta
     8641 tttaactaaa tcaggtcatt ttataagttg gatttcatat gcttctaagt tagaagagat
     8701 aattgctaca gtgattattt tatatgtttt agttttgttt attatatatc ttagtaaatc
     8761 tgaaactaca tctaaagcta aataagaatt attaaatatg tgtatttaat aggaatattg
     8821 atataaatat atgaagataa gataaaccat agaaacttaa tttagcttaa ttttattgga
     8881 ggaaaacaaa tgaaaacaaa agaagaatat ttaaaagaag ttagaaacat atcagaagaa
     8941 tactttagaa aaggagagtt tttttgctct gaagcagttc tccagacaat aaatgatgct
     9001 ttaggccaac ctttaagtcc agagataact aagttagcat caggatttcc gataggactt
     9061 ggaaaggcac aatgtctttg tggagcaata tctgggggag agatggcttt aggtatagta
     9121 tatggaagag ttcatggtga agctatgaat ccaaagatgt ttgagcatgc taaaggtttg
     9181 catgattata ttaaaaaaga gtatggtgca acatgttgta gagctattac aaagaaatgg
     9241 gatggtgata actttatgtc tcccgaaaga aaagagcact gtattaaaat aacaggtcaa
     9301 gttacagagt gggttgcaga taagcttata gaagatgaaa aacttaatat caaatagtgt
     9361 aatattagtt ggtgaattta taaagttaaa tttacttata gtataagata cttgatattc
     9421 tcgtattgat agaaaaattt atagcatatg tatttaatga aatatactta tatttagagt
     9481 gtggtttaat ttcataaata catatgctat tattttaagt atataagcaa atttatttaa
     9541 ataaatagaa acagttaatt tatatgtaca aaactatata tgattagtgt aaataaatac
     9601 ctattttata atactcaaca taaagacacc agacatgatt agtattatac caataaaaat
     9661 agcaagccat tcttttttgt tcttcttttc tttaaaaaat ataatccctc ctaaagtggc
     9721 tactatcaca caactctgag agatagaaaa acttattgta actcccaatg tattagtcgt
     9781 tatgaacata cctaaatttg ctattgacca caatatacca gtaattaaat tgaataaaac
     9841 attttcccta tatacaatct ctttagaaaa atacttgaca ataatagatg aagttatcaa
     9901 cataccaaca gattgaggta atataattct atatccttca attaaaaata tttgattagt
     9961 tacaacatat aatgataaaa atagagatga taataataca actccaacac attttaacat
    10021 atttttatct gtagattttt tagtttgata atttgtgaga agtataccaa gtattataca
    10081 taatattgat aaagaaccaa tcagtaacat tttagaactg gtccattcat taaacaagat
    10141 tacagctaat aatgtcgtaa aagttaattg tgaaccattt gaaattggca ttactttaga
    10201 tacactagca atttgaattg ctttaaactg cattaattgt cccaaactcc agaatatgcc
    10261 agaaatcata cttatacaaa atgaaaaaaa tgtaatttca ggatgtataa ccagagttat
    10321 tatagtggaa aatataaacg cacttatgga tgtacctaaa agctgttcat gaggagaact
    10381 tttctttaga tttgcaacta tgggcatcaa tccccatccc attacaggaa gtaaagcaat
    10441 aattattgtc agcattaaac tatttccttt catattaaat ttagacagct tttaatttat
    10501 catctaaaat taaagtgttc aatacataaa aattaccgta tttaatcagg aaatattagt
    10561 agttttttag aaaaacattt tccattaaaa taaagacaaa gggaaggagt aaaattgaaa
    10621 atgttaactg atatgattca aaatcgtgaa attaaaataa taaagttttt attaaaagaa
    10681 ggtcatacaa ctgtaaaagc aatagcagat gatttagatg tttcagaaaa aacagtttca
    10741 aaatctctta aagaagtaga agcagtgctt aaaaagatag gtctttcttt aataagaaaa
    10801 cctaagatag gtgtttatat tgatggagat ttaaaaaaaa tacttcctta cctagataat
    10861 aaaggaagtc aaattcctac aaccagagag gagagagtta tctatatata ttcagtactt
    10921 ttaaagagta atgattatat tactatacaa caactttctg atgaactata tataagtcgt
    10981 ggaactattg aaagggattt agcagaagtt gaaaaactgc taaaaaacga agggatttcc
    11041 atatataaga aacctagtaa gggaataaag ttaaatataa gtgaaagaga taaaagacaa
    11101 cttactgcta agtttataca taaattttgg agtagaaact ggtatattaa gcaagagcaa
    11161 gaaagctttt atcaagcatt tgaaaagata caagcagatg taaatggtat tttttcggaa
    11221 gatgatttat caataattat tgatattctt aggaatttca gcagagaaag aaaatttgag
    11281 ttctctgatt atgaatttca gtcattagtt attcatttgg caatagctgt agagagaatt
    11341 agaaaaggcg aatatataga agaaagtttg tatactggta atatagaaaa taatcaaatg
    11401 tcaaatacca attatttagc aaaacaatta gaagaaaaat tttcaatatt attaccaaaa
    11461 tctgaaattg gatatataaa tttacattta gtagcgtctg accaacatag agatactaat
    11521 gaactaaaaa atacttcaac taatccttgt gagacaagta caaataatat atcaaatctt
    11581 cgggaaatta taaaaaatac attgtatgag acagcttatg acaaggattt aattgatgga
    11641 ttaacttcac atgtacattc atcaataaat agactgaaat atggacttag tattaaaaat
    11701 ccatatgtaa atacaatcaa acaaaacttt tctttatcat ttgagcaagc tttagattta
    11761 aagaaagtaa ttgaatttat atatgatatt aggataaatg atgatgaatg tgcttatata
    11821 gcattacatt ttgaagcttt tagagaaaga atgaaagaac ttccacaaaa tattagagta
    11881 attctagtat gcagtacagg attaggttca tctaggttat tgtctgcaag aataaaaaaa
    11941 tactttccaa tgataaagat tgaaaaagta ctttccgtac aagaattagt atccagtaaa
    12001 attgatgcag atttagtaat aagtactatt tatttagagt tagaaacaat tccaaccata
    12061 gtagttagtc ctattttaaa tgaaagtgat ataagaacct tggaaagaaa tatacaaaat
    12121 gtttcaagtt atacttctaa gaagtataaa tctttcagaa acttaatctt tgaagaaaat
    12181 atattgttga atattgaagc ttcaaactat gaagatgcaa ttaagtatat aacagataac
    12241 ctaataaaaa aaggttttgc aaaagaaggt atagctaaaa gtgcaattga gagagaaaat
    12301 ttatcttaca cttctttcca atcaatagct acacctcatg ctaatcctag ttatattaaa
    12361 aaatctaata tatctgttgc aacgttaaaa aatcctatta catggggaaa tgaaaaggtg
    12421 gaagttattt tctttatttc attagaaaat gacaaaaacc tagagcttga tgaaatatat
    12481 gaagtttttt atgacttcat tgacaataaa aaaaatataa atgacatatt aagtgcaact
    12541 tccacaaagg aagtgtacga tttgctagtg gaggagagta tatagtgaaa atatcagatg
    12601 tattgaaaaa agagcaaatt gtttttaatc ttgatacaaa tacaaaggaa gaaacaataa
    12661 aaaaattaag tagtatattt gttgaaaatg ggattgtgga caatgaagaa gaatatgtta
    12721 agtctgtttt ggaaagagaa gaacattcaa ctacaggtat tggtaatggt attgctattc
    12781 ctcatgggaa aagtaatgct gtcaaaaaat cttctataat atttgcaaag actaagcata
    12841 agattgaatg ggatgcttta gatgaaaaac cagtagatag tattttctta ttggctataa
    12901 gcaatattga taaggatagc aatcatcttg tactattatc taaattagca actaaactta
    12961 tggatgatga caatgtagat gcactaaaat tagccaattc agaacaagaa ataatcaata
    13021 tatttagtga agaaggagag atgtagaaat gaaaagaaaa attatagcag ttacagcatg
    13081 tgcaacagga gttgcacata catatatggc agctcaagcg ttaaagaaag cttcaaaaaa
    13141 gatgggtcac cttataaaag ttgaaacaca aggagctaca ggaatcgata atgagttaac
    13201 tacaaaggat gcacaaattg cagaagtagt aatatttgca gtagatacta aagtcagaaa
    13261 tgctgaacgt tttgagggaa aagaaatact agaagtacca gttaatgcac ctataaagga
    13321 tgcagagaaa gtaataaatg atgcactaaa gttaattgac actaaataaa tggagggaaa
    13381 tgtaatgttg aaaaagatag tctctgagtt aaaaaagcat gtcttaactg gaatctcata
    13441 tatgattcca ttagtaattg caggtgctat gataatggcg atatctcgtg taggtggttc
    13501 tttctataat ataccagata tatgggatgc taaatatgca gaaagtgcta gtagtattgt
    13561 tagattatta catgatttag atgggtttgg aggtactgca ttaggattaa tgtttcctgt
    13621 aattgctgca tttataggat tttctatagc tgataaatta gctattgtac caggtcttgt
    13681 tggaggtatg attgctaaag atataggtgc aggattttta ggagctttgg cagttggtct
    13741 tatagcagga tatacatgtt tgtttattaa aaatcatgtt aaattaccaa aatcagcagc
    13801 ttcaatagta ccaatattta tagtaccagt atttgggaca ttaatcactg ttgtattaat
    13861 caattatgtg ataggtatac cttttgccac attaaatact ggattagaaa attggttaaa
    13921 tggattatct ggtacaaatc agatactaat ggcagctatt ataggggcta tgatggcagt
    13981 tgacttaggt ggaccagtta ataaagcagc tttaacaaca tcacttgcat tattaacaag
    14041 tggaatttat gcgcctaaca ctgctgcaat ggtaggaata gttataccac cactaggatt
    14101 gggacttgct actatactag caaaacaaaa atataataaa cagttacgtg aagctggaaa
    14161 atcatctctt ataatgggtt taattggagt aagtgaagga gcaattcctt ttgcggtaga
    14221 atctccatta aaagtaatac cttcatgtgt aattggaaca gcaattgctt cagctatggc
    14281 agttggttta ggttcagtaa atgcaactcc aattagtgga ttttatggat ggtttacagt
    14341 agagaattgg ccaatgtatg tgttatcaat agcagttgga acagtaattg tagctggatt
    14401 agttatagta ttgcgtggaa atccagtgat tgaggatgat gaatttgaag atgatgattt
    14461 tgatgaagca gagtgggaat cttaaaattt aataactatt taagacgtgt tataagctat
    14521 attcaatatg aatcttatac taaataaagt gttaaacatt cagtttagac ttaaatataa
    14581 aagataaaga acagaggaga ataaatgata aaagcacata ttgttaatca tacacactgg
    14641 gatagagaat ggtattttac ttctggagat gcattagtac ttagtgaaca actatttaca
    14701 gatgtaattg atgaattaga acgtaatcca gatgtaagtt ttgtacttga tggacaactt
    14761 tctattttgg atgattatgt acaattacac aaagaaaaaa tagaaatcat taaaaagtta
    14821 ataaaagaag atagactaca tattggacca tggtttactc aaactgatgc attctttgct
    14881 agaggagagt ctattttaag aaacttaatg attggaattt ttgaaagtaa aaagtatggt
    14941 aagtatatga aaattgggta tttgccagat acatttggat ttaatgcaca gatgcctata
    15001 ctattaaagc atgtaggtct tgataatata ttcttttgga gaggtataca tcttggtaaa
    15061 caggttcaat caccatattt taagtggaaa agcttaaatg gagaaagtta tatatatgca
    15121 gtaaatatgc cacatggata tggaactggt atgcttttag agccttcatt aaagtatgtt
    15181 aatggacgct tagacccagc tattgatttt attaaatcat atactgatgt agatgaggtg
    15241 ttaatacctt ctgggaatga ccagttaaat ataataggtg atttcaaaaa taaaattaaa
    15301 gcgattaatg atatcggaaa gtattcatat ataacgagtt catatcaaga gtttttaaaa
    15361 tatataaagt ccatagaatc actagaaagc tatagaggag aatttagaga acctgtatta
    15421 gcaagaatac ataaatcaat tggttctagt agaatggata taaaattagc atgtgataaa
    15481 ctagaaaaca agctgattaa aagaatagag ccattattag taattgctaa aaagagtaaa
    15541 attgaaataa gcaatcagtt acttataaat acatggaaaa aacttttgga aggacaagca
    15601 cacgatagtt tagctggatg tgtaactgat actgtaacag atgatattct tcaccgtata
    15661 agagaagcaa atgaaatatg tgacagtatt gaaaatacta ttgtaaaaaa gatttcagaa
    15721 gggcttaaat tatctaaaaa tgatatttta gtatttaata cagaagctaa gcgttttaat
    15781 ggatataaag agatacaagt tgtaagtgat agtaaaaata tttattttaa agataattta
    15841 gatgctacta taattgaaga aacttatgta aaaccgagag aaaatgtatt agaagaaaca
    15901 cctgctggta atatatttat tgaagaacca ggatattata tattaaaagt aagaataaat
    15961 gtacagcttc ctgcacttgg ttataaagtt gttagttttg aatcaagtga taaagaaatg
    16021 gtgtcactta ataaatctaa tgatgctttt atatctaatg ataattataa gataatatat
    16081 gaagatggaa gcttaaattt acaacttaaa gatggaactt acatatcaaa ctttttaaca
    16141 ttaaaagata gtggtaatgc aggtgatacc tatgactttt ctccactaaa aaatgatgag
    16201 gatataaaac tttcgtttga taaagtgtat acagaaaaag ctttaggata tgaaaaaatg
    16261 gtggttaaag gtactacttt actaccactt acgttagaag atagaaaaaa caaatatctt
    16321 aatgggaaat taaatgtaaa agtaagtatt gtcttatcca aagaaaatcc attgatggat
    16381 gtaaaacttt gtgttgataa tacgatatat aatcatagac tacgtgttca tataaaaact
    16441 gatataaaag ataataaaaa catagcatca ttgccatttg gatatataac aagagaaaat
    16501 ggagttttag acaactggga agatatttac agtgaaatgc ctatagattt agaacctttg
    16561 gaaagtaata ttactctaac aaaccaaaat agaagctgta ctatatttac taaaggaata
    16621 aaagagtatc aacatattga agatgaaatt gcattgacct tacttgctac tacagatgaa
    16681 ctaggaaagc ctgatttatt atatagacca ggacgtgctt ctggtgatac aactaaaaaa
    16741 ggtcacataa gaattaaaac agataaagct caaatgctta agaaattaga attttcattt
    16801 gcaatatata tggattctaa atcatttgat gaattggaaa ttgcgaattt aacccacaat
    16861 tatctaaggg aatgtgtaaa ttatcaatta caggattaca atttcttttt atatcgtata
    16921 gataataaga ttcaaaaatc aattgtcaat aaaactgtaa gtagagaatt agaaataata
    16981 tctttaccag aaaactatct tatatcagca tgttatcctt cttattacag taaagataaa
    17041 tttataataa gagtggaaaa tccaagtagt aataaaatga tattggatga gcaaatattt
    17101 aaaaatagaa atgggaaaat tgtaaatgca atagaagatg tagaagagga acaggtatat
    17161 gaaatagcac cttttgacgt tatttcaata ttattggatt tataagtgta aaagtaagtt
    17221 tattgtttaa agtctgacag atattaatct gccagacttt ttaatttgta taaatttggg
    17281 taaatattaa tgaatggttt tagtatttaa gtatgaattt agtatttaat atagaattta
    17341 ttgaagtaga ataattagtt taacaggata agcagtttaa ataaagaatt attatattag
    17401 agtgatttta tatataattt aagataataa aattttaata aaatgagtta aagattgtat
    17461 attaatagag ttgatgtagt tacaaaaaag ttaccaattg ttatgtattt acaaaactga
    17521 tatattaatt acataaaatc taaaatag
//
LOCUS       DAMJVK010000084        17446 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.085, whole genome shotgun sequence.
ACCESSION   DAMJVK010000084 DAMJVK010000000
VERSION     DAMJVK010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17446
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.085"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            141..503
                     /locus_tag="PC414_002830"
     CDS             141..503
                     /locus_tag="PC414_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HDF2465215.1"
                     /translation="MIKLYGYTKCSTVKKAKNWLKENNLEFEDIDMVQNPPSKEELKS
                     IYKTSGYDIKKFFNTSGMKYRELGLKDIVKTESDDKLLEILVSDGMLIKRPLLLDGKN
                     VLLGFKEDVWKSTLLKED"
     gene            939..1640
                     /locus_tag="PC414_002831"
     CDS             939..1640
                     /locus_tag="PC414_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4253 domain-containing protein"
                     /protein_id="HDF2465216.1"
                     /translation="MSIEDLYDFECVPLNNEIDIDVLFEKMIEDGKEKEYTPIIVIDE
                     KVGLLRKNIDMITKEYGSLENYREYCLTKYNEIDVNQFFDFRKSEISELLKELIDLED
                     DFYDYKHFRPIMNIDIFHEYNKVYIAKIPTIKPYEVFAYIPIGGFNDCPRDEEHIAIA
                     KYWYEKHGIFPIAIGCDTVQYSVKNLTKDGKKFDELCIELVFYCEDIIIQGYETLKAL
                     KDVLKRSTIVLFWWD"
     gene            1911..2729
                     /gene="larE"
                     /locus_tag="PC414_002832"
     CDS             1911..2729
                     /gene="larE"
                     /locus_tag="PC414_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HDF2465217.1"
                     /translation="MEVNFVKEKEKLDKLKKMLLELGSVVVAYSGGVDSNFLLKVAKD
                     TLGENVIAVTIHAMMHSSREIEEAKQYTQNFGVKHIILNIENFDLKEFKENGVDRCYH
                     CKKYIFSKIKEVAKEHNIKYIVDGTNIDDLGDYRPGLKALSELGVISPLKDSSLKKEE
                     IRSLSKTLGLNTFNKPSFACLASRIPYGVEITDENLRIVEKSEEYLSNLGFSQFRVRM
                     HGDIARIEVGQEELGKFFENNNFNKVDTKLKIFGFKYVTLDMSGYKMGSMNLNV"
     gene            2784..3932
                     /locus_tag="PC414_002833"
     CDS             2784..3932
                     /locus_tag="PC414_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HDF2465218.1"
                     /translation="MKNYTLISPCFFGMEKMLAREITNLGYEIIKTEDGRITYKTDEF
                     GIAKSNMWLRCAERVHLKIAEFEAKSFDELFENTKRINWSRYIPYGAQFPISKASSIK
                     SKLYSTPDVQAIVKKAIVESLKKSYLEDGLLKEDKEKYPIFVFIHKDKVTISIDTTGD
                     ALHKRGYREKANKAPIRETLAAGLIYLTPWKAGRVLVDPMCGSGTILIEAAMIGINMA
                     PGLNREFISEKWRTLDKKIWWDVRKDAFNKIDNESKFKIYGYDIDEESIDIARENAEI
                     AGVDEYIEFNVGDATQFKSEDEFGFIITNPPYGERLEDKDSVKQLYKELGYAFRKLKN
                     WSYYLITSYEDFEYEFGQKADKKRKLYNGMLKTNFFQYPGPKPPRNNK"
     gene            4068..5456
                     /locus_tag="PC414_002834"
     CDS             4068..5456
                     /locus_tag="PC414_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465219.1"
                     /translation="MKVLKLITVVALMMLFVTGCNIKTESPEQLAKIPDYDDTKKVLY
                     DGIDQLLKPDSSMILPSNTKEVGKINKVDLNSDGIDELVVFEQKEDLSNNVSQVGFVT
                     LSYNGEKYVLGDHFLENGESIEYANFYDLDSDGYKEVILLVKSKDKTNMHIYKVVDNE
                     ITKIYDLDASWLQNKEDFNDMKVKIGYIDGDDKLDILMLHLNNKTNEMFASVANFDGK
                     MKIKDSVKFENVKNLSELYITLGNVASSVGNSSAAIKGIVLDIPILKDNNYITQILYM
                     KDGKINKAFSDYDKTITKSYYIPVDDIDKDKIIEIPIVAGSTGNNKNTYSSKSSATIS
                     WYRWNGKEGDDSSLIFTSQIYYNYKYNFKLFIPNNLFDKILVEQEFVGEKAVFKFYYF
                     DYSDRKNLFNIVVESKNKLEDRKNTGTQNSIVLQESDEYTFLLVVNNANEMDKLDMTI
                     DALKEYFSLIYG"
     gene            5485..6183
                     /locus_tag="PC414_002835"
     CDS             5485..6183
                     /locus_tag="PC414_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2465220.1"
                     /translation="MKEKILILEDEIGIRSFVSINLKREGYEIVEAGTGREAIEKMTT
                     EKDITIALLDVMLPDISGIEVCKFIRENFDQVGIIMLTAKAQEDDKIEGFISGADDYI
                     IKPFSIKELLVRVSALLRRVAKDDSSVKSSEIVSPPFILDIDKRKLFKNGKEIELTPT
                     EFSIVKYLISNAKQSLSRDQILDEVWGTNYLYDFKIVDVNIRRIRNKIEDDPSKPKYI
                     QTIWGYGYCFRKEE"
     gene            6186..7619
                     /locus_tag="PC414_002836"
     CDS             6186..7619
                     /locus_tag="PC414_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2465221.1"
                     /translation="MLHFEGLRKKVIKNYFIIIIIMVTLFEGLFMFYIQNYYYDSVKQ
                     LLESEIKYADEYNAITMETTSFEKKVKNIFDKQPLTKNSEFGISIIDKDKNIILDQYG
                     FKSKEKANYEDVNNALKDIKTKNLTPYTYRIPDTGEHVMSISLPLKVNNIIEGVVRYT
                     VSLDAIDNAILKQATWLILAGIFILIIAILISLKFAETLIKPLRELKKFANELAVGNY
                     NIKLEKMKIVDDEIGDLAQTFEHMAHEIDKSEKLKEEFISSVSHELRTPLTSIKGWSE
                     TLGYESITREELDLGLGIIQDETERLIKLVEELLDFSRLSSDRIKLHVDIVDVEGLIV
                     GVVNQLKVKAAEKDISLLFEFENEFIENIQGDKNRLRQVLINLIQNSFKFTSQGGYIK
                     VVASQDEEITTISVEDNGSGIEKQNLNKVLDKFFQEDYNKAGSGLGLAISNEIVKLHG
                     GRMKIESEKNVGTKITFNIKNKFAKQA"
     gene            7641..8378
                     /gene="truA"
                     /locus_tag="PC414_002837"
     CDS             7641..8378
                     /gene="truA"
                     /locus_tag="PC414_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDF2465222.1"
                     /translation="MRNIKMIVAYDGSRYKGYQKLGDNNMTIQEKLENVLSKMTNETV
                     EIIGSGRTDMGAHARGQVVNFRTNCMDSLDKIQKYLYEYLPEDIVVKTVEEVDERFHS
                     RYNVKSKTYMYKIDNNKYHSPFIRKYATHVSKKLDLDRMRKASEYLVGEHDFTSFASS
                     KSKKKSNVREIYSINIKEDDNVIEIYVEGNGFLYNMVRIIVGALIDVGLKRRAPQDIK
                     YMLESKDRCQSSDTAPAKGLCLWKVTY"
     misc_binding    8489..8696
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8949..10148
                     /locus_tag="PC414_002838"
     CDS             8949..10148
                     /locus_tag="PC414_002838"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HDF2465223.1"
                     /translation="MKEKVVLAYSGGLDTSIIIPWLKENYEDIDVIAVCGNVGQEDKM
                     EDVYEKALQSGASKAYVDDISEEFVTETIFKAVKAEAKYEGKYLLGTSLARPIIAKKL
                     VEVAHKEGAKYICHGCTGKGNDQVRFEATIAALDPTIKVIAPWRIWDIKSREDAIDYA
                     EKHNIKVTATKAKIYSVDANLWHVSTEGGDIEHLENEHKKDVYKQCVDPEDACDVAEY
                     VEVYFEKGVPKKVNGEELSPVALIHKLNELGCKHGIGVIDIVENRLVGMKSRGIYETP
                     GGTILYEAHNILESATLDKDTLHFKQMVSYKYGELIYNGLWYCKLRESIDAFMEQTQD
                     NVTGTVKVKLYKGNIKPAGIFTENALYDEGISSFGNSELYDHKDAEGFINLFTLPLKI
                     RAMKAGK"
     gene            10395..11441
                     /locus_tag="PC414_002839"
     CDS             10395..11441
                     /locus_tag="PC414_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4872 domain-containing protein"
                     /protein_id="HDF2465224.1"
                     /translation="MRKIIEIKHHSHDYECMWNGIEDLYINKTGECLPNDFFFLLSGF
                     GSFCYMKTNKADLKRMVALGDGRTKQMYKFLAPIVGFEYKHHEFKKYEQALKKAKSEI
                     DDGYPVILGALDMYYLPYYKKLYHKEHIPFHYILMVGYDDEGIYLYDCGRTELLHLSY
                     DKLRESMNCNSPGLSKENTICTIRMNTSKNKYNIAREAIAIKRDMFLNPPKGFLGYKG
                     FEKFIKELPQWNTELDKDDYDKILTNMVMFFGTVPTIPNAIKGIKEPDTVQFKGGFDK
                     MSRMLNSIGEEYKNDSFIQVADIFDKTAIIIEKISNIIIDYLTQTCDDTGQLPLLFSE
                     VLEHMKTGYLILDV"
     gene            11549..12712
                     /locus_tag="PC414_002840"
     CDS             11549..12712
                     /locus_tag="PC414_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2465225.1"
                     /translation="MRTVKQVSDLTGISVRALHYYDEIGLLKPNKITDAGYRLYDDES
                     IKTLQQILFFKEIDIPLREVKEIMSSQYFDKVEALKNQKKLLILKRKRLDELIELINQ
                     TLRGEGNIDFKEFDMSEYFDVLEEFKREHRNKVIKIYGSVEKYNEYIERVKSNEEKIA
                     KMAIKQYGTIEKFAKAIKTNFSSDILNLGEKFDRYKNDCLKEKHPKLKELYRKLVEDL
                     NRNPSSTDLQEIAKEITDISKKDYEIFSMDTGDDNWYYMVQNYLVNPMWIEEVDKKYG
                     SGAGKFIGQVLKTYLRDRKPKINTLYEKLVEDLSRDCSSREVQSIVEEIDNEMKRSNE
                     FYKIDNGERYFDYMSELYLQDSNYIKVTDKNYGDGASKFIGEAFKIYFDNNNC"
     gene            complement(12801..13181)
                     /locus_tag="PC414_002841"
     CDS             complement(12801..13181)
                     /locus_tag="PC414_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4363 family protein"
                     /protein_id="HDF2465226.1"
                     /translation="MKSTTFVILWTILFVLFGFYVNNKLYDFTEGYKDNISVLEKSIE
                     NEDWEKAQKEADSISTSWNKEKNHWYKVLNHEYFDEIGLRFNILDKAIYTENKLKSLE
                     EVESIKTYLGNIIESVKFDVNYIF"
     gene            complement(13184..13843)
                     /locus_tag="PC414_002842"
     CDS             complement(13184..13843)
                     /locus_tag="PC414_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HDF2465227.1"
                     /translation="MTVVLIRSIILYITVLIALRVMGKGEIAEMNCFDLVITLLIAEV
                     ASVPMENNNIPIINGVAAISGLVIMQTLISFLSLKSRKLSSFLSGKPSVLIDKGKIVY
                     KELKKERISIDELLEQLRIQGYFNLKDVQYAILETDGNLSVVPASSYNSTPPRAFNHL
                     PIPLILDGRIINKNLDIAQKDTNWLMGILKSNHIETFKDVLICVLDENDKIFIQNKKG
                     D"
     gene            complement(13949..14923)
                     /locus_tag="PC414_002843"
     CDS             complement(13949..14923)
                     /locus_tag="PC414_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HDF2465228.1"
                     /translation="MKYKLFIDFEFNILDDNKNKPKEYNGAELISIGGVLVDNEFNTI
                     DNYYSLVKPKYNKILSNKCKNLTKLNQLDINNAPNLLYVMDDFYKWFCKFDDVTIYNW
                     GDFDITGLLTSFRVYKYTGKCLELFNMMIDIQPFISQHITYNNRILSKQISLLNMKKI
                     FSVEGDIKHNSLSDAVDLMNVCKCFFFNSPKNIDILEELYNKLPALKTPLYYIPYFED
                     ENFELKFDRTPEDIIIYLKQILTILNISKKEIYFKKRSVLVNNSKIINFKNLSSSFKL
                     IKLNEFEEYPDFILKLGDKKSFVESVIKINKSNRKSIKNLILKLSKKP"
     gene            complement(14963..15310)
                     /locus_tag="PC414_002844"
     CDS             complement(14963..15310)
                     /locus_tag="PC414_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465229.1"
                     /translation="MLNLKEIREEIIKKYIGDSLISELTEELKNILITDLQSNPFKSN
                     IELSIIPANIEIFKKIENILIDSKVNLKMGWERILINYILKDLFELNYEINKSCNEPL
                     MFRVIISIDSYDA"
     gene            complement(15528..16484)
                     /locus_tag="PC414_002845"
     CDS             complement(15528..16484)
                     /locus_tag="PC414_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HDF2465230.1"
                     /translation="MKKILITGALGQIGSELTIKLRNEYGEQNVIASSRRVKEGNPVC
                     ESGIFEILDVTDKNRFFEIAKKYDVDTIIHLASLLSAVAESKPLEAWNLNMNGLINGL
                     EIAKELDCKFFTPSSIAAFGENSPKNMTPQDTLQRPNTMYGVTKVSGELLCDYYHSKF
                     GVDTRGVRFPGLISYVTPPGGGTTDYAVDIYYEALKNKRYKSYIAEGTKMDMMYMPDA
                     LQSIVDLIEAPADKLIHRNAFNITAMSFSPEEIADSIKKYIPDFVIEYDVDPVRQSIA
                     DSWPNSLDSSSAVKEWNFKFSYDLDKMTKDMLEKLSEKGIGK"
     gene            16929..17297
                     /locus_tag="PC414_002846"
     CDS             16929..17297
                     /locus_tag="PC414_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HDF2465231.1"
                     /translation="MTRPSIYHSQGYTCAEALIKSYNEEHNTDIPISIGSGMGVGMNV
                     GSVCGAVNAAVVIIGYLNGRNSNLDENIARKYSRELLNRVRNKYSSEICVSLKKNKVT
                     CEEIINFTYDALNDMLKNQK"
BASE COUNT         6897 a         1807 c         2813 g         5929 t
ORIGIN      
        1 atataatgtg aataaatgtt taataatttg aaatatgata attttttatg ttgaaagctt
       61 ataatgatta tgatataaat tggttatagt atacaattta tgggtataac aataaaaaat
      121 tagaaaggaa tttttataaa atgattaaat tatatggata tacaaaatgc tctactgtta
      181 aaaaagctaa aaattggctt aaggaaaata atttagaatt tgaagatata gatatggttc
      241 aaaaccctcc tagtaaagag gagttaaaat caatttataa gactagtgga tatgatataa
      301 agaaattttt taatacaagt ggtatgaaat atagagaatt gggtcttaaa gatattgtaa
      361 aaactgaatc tgatgataaa cttttagaaa tattggtaag tgatggtatg cttattaaaa
      421 gaccattact tttggatggt aaaaatgtat tattgggctt taaagaagat gtatggaaaa
      481 gtactttgct aaaagaagat taggaagaga taggtgtgtt gattaaaatt attaattaat
      541 acacctataa atttatgtat taaatctaat ttttataaat attaattagc aaattcaaat
      601 caaaaaaatt catatcaaca aacatattac ttcttattac aaattatatc atatgtttta
      661 tattaaaata ttataatatt acaacgaatt gttacaaaat tacttattat taaccatgaa
      721 catttgtttt gtgctatcat atttatataa gggaattgtc ataaaaaata tcttgttcat
      781 acagttttat atagaaaaaa gaaatgaatc ttttctttat ataagtatat tttgtaaatt
      841 gcctctatat ttatagttta aataatagtg gtattaaaaa tctgacaaca aaaataaaac
      901 tttcaaaaaa ttaaatctaa aaagaatagg agataaatat gagtattgaa gatttatatg
      961 attttgaatg tgttcctttg aacaatgaga ttgatattga tgttttattt gaaaagatga
     1021 tagaagatgg aaaagaaaaa gaatatacac caattatagt cattgatgaa aaagtagggc
     1081 tattaagaaa aaatatagat atgattacta aagagtatgg ttctcttgaa aactatagag
     1141 agtattgttt aacaaaatat aatgaaattg atgtaaatca attttttgat tttaggaaga
     1201 gtgagatttc agaattatta aaggagctaa ttgatttaga agatgatttt tatgattata
     1261 aacattttag accaattatg aatatagata tatttcatga atataacaaa gtttacatag
     1321 ctaaaattcc aacaattaaa ccatatgaag tatttgcata catacctatt ggcggattta
     1381 atgattgtcc tagagatgaa gaacatatag ctattgcaaa atattggtat gaaaagcatg
     1441 gaatctttcc aatcgcaatt ggatgtgata cagttcaata ttctgtaaaa aatttaacta
     1501 aagatggtaa aaaatttgat gaattatgta ttgaattagt attttattgt gaggatatta
     1561 ttatacaagg gtatgaaacc ttaaaggctc ttaaggatgt attaaaacgc tctacaatag
     1621 tgcttttttg gtgggattga tattccatca tttagatagt attaaattgt tagtcaccaa
     1681 gaatccactt gctttagtta tggggagtgt gtcaaactag gtttttatac acagtgtagc
     1741 atagtaatat gtttaaatta caaaggttaa aaaaatactt taatattttt aatattataa
     1801 ttaatagatt taaaggtaat tataatttat aaaataaata ctttatatgt tataatcatt
     1861 aagaatgtaa aaaaataata tatagtttta tagttcatat ttttccagat atggaggtaa
     1921 attttgtgaa ggaaaaagaa aaattagata aattaaaaaa aatgttactt gaattaggca
     1981 gtgttgtagt tgcttattct ggtggagtag atagtaattt tttgcttaag gtcgcaaaag
     2041 atacattagg tgaaaatgtt atagctgtga ctattcatgc aatgatgcat tcaagtaggg
     2101 aaattgaaga ggctaaacaa tatactcaaa attttggtgt taaacatatc atattaaata
     2161 tagaaaactt tgatttaaaa gaatttaaag aaaatggtgt tgatagatgt tatcattgta
     2221 aaaaatatat attctctaaa ataaaagagg tagcaaaaga acataatata aagtacatag
     2281 tagatggaac aaatatagat gatttaggag actatagacc tgggcttaag gcattgagtg
     2341 aattaggagt aattagtcca ttaaaagata gtagtttaaa aaaagaagaa attaggtcgc
     2401 tttcaaaaac attaggatta aatactttta ataaaccatc atttgcatgt cttgcgagta
     2461 ggatacctta tggagttgaa ataacagatg aaaatttaag aatagtagaa aagagtgaag
     2521 aatatctatc caatttaggt ttttctcaat ttagagtgag aatgcatgga gatatagcta
     2581 gaatagaagt tggacaggaa gaactaggta aattttttga aaataacaat tttaataaag
     2641 ttgatactaa attaaaaata tttggcttta aatatgttac actagatatg tcaggatata
     2701 aaatgggaag tatgaattta aatgtgtaga ttattttaaa aataaataag ttttatagtt
     2761 aatataagga aacaggaggt tttatgaaaa attatacgtt aatatcgcca tgtttttttg
     2821 gaatggaaaa aatgctggct agagagatta ctaatttagg atatgaaata ataaaaacag
     2881 aagatggaag aataacatat aaaactgatg agtttggaat agcaaagtca aacatgtggt
     2941 tgagatgtgc tgaaagagta catcttaaaa ttgctgaatt tgaggcaaaa agctttgatg
     3001 aattatttga aaatacaaaa agaataaact ggtctagata tataccttat ggagctcaat
     3061 ttcctatatc aaaagcttcc tctataaaat caaaattata cagtacacca gatgtacaag
     3121 ccatagtaaa aaaggctata gtagaaagct taaagaaaag ttatttggag gatggtttgc
     3181 ttaaagaaga taaggagaaa taccctattt ttgtatttat acacaaagat aaagtaacca
     3241 tttctataga tacaacagga gatgctttgc ataagagagg atatagagaa aaggcaaata
     3301 aagctccaat aagagagact ttagctgctg ggcttattta tttaactccc tggaaagcag
     3361 gtagagtttt agtagaccct atgtgtggtt ctggtactat attgattgaa gctgctatga
     3421 ttggtataaa tatggcacct ggcttaaata gagagtttat atcagaaaag tggagaactc
     3481 ttgataagaa aatatggtgg gatgtaagaa aagatgcttt taataagata gataatgaat
     3541 ctaaatttaa gatttatgga tatgatatag atgaagaatc tatagatata gctagagaaa
     3601 atgctgaaat agcaggtgta gatgaatata tagagtttaa tgtaggagat gcaactcaat
     3661 ttaaaagtga agatgagttt ggatttataa tcactaatcc tccttatgga gaaagattag
     3721 aagataaaga cagtgttaaa cagttgtata aggagcttgg ttatgcattt agaaaattaa
     3781 aaaattggtc gtattatttg ataacttctt atgaagactt tgaatatgag tttgggcaaa
     3841 aagcagataa aaaaagaaaa ttgtataatg gtatgttaaa aactaacttt ttccaatatc
     3901 cgggtcctaa gcctcctaga aataataaat aagttttaca tgatttattt gtaaaatata
     3961 aaattttatt aaaataagta aaacattatg atgccttgaa acgtctaatt attgtgagga
     4021 gatttttgtg aacaaattta aagatttata ggattaggag gagttaaatg aaagtgttaa
     4081 agcttattac tgtagtagcc ctgatgatgt tatttgttac tggatgtaat ataaaaactg
     4141 aatctccaga acaattagcg aagatacctg actatgatga tacaaaaaaa gtattgtatg
     4201 atggtattga ccagttacta aaaccagatt catctatgat attgccttct aatacaaaag
     4261 aagtaggaaa aataaataag gtagatttaa acagcgatgg aattgatgaa ttagtagtat
     4321 ttgaacaaaa agaagattta agtaataatg tgagtcaggt agggtttgta accttgagtt
     4381 acaatggaga aaaatacgta cttggagacc attttttaga aaatggcgaa tcaatagaat
     4441 atgctaattt ttatgatttg gactcagatg gatataagga agttatctta ttggttaaat
     4501 ccaaagacaa aacgaatatg catatatata aagtagtaga taatgaaata acaaaaatat
     4561 atgacttgga tgcatcatgg cttcaaaata aagaagattt taatgatatg aaagtaaaaa
     4621 ttggatatat agatggagat gacaagttag acatattgat gcttcatttg aataataaga
     4681 ctaatgaaat gtttgcaagt gttgcaaatt ttgatgggaa aatgaaaatc aaagattctg
     4741 ttaagtttga aaatgtaaaa aacttaagtg aattatatat aacattaggt aatgttgctt
     4801 catctgttgg aaattcttct gcagctataa aaggtattgt acttgatatt cctatattga
     4861 aagataataa ctatattacg cagatacttt atatgaaaga tgggaaaatt aacaaagctt
     4921 ttagtgatta tgataaaact attactaaat cttattatat acctgtagat gatattgaca
     4981 aagataagat tatagaaata cctatagttg caggcagtac aggtaataat aaaaatactt
     5041 atagttctaa atcatctgca actataagtt ggtatagatg gaatggaaaa gaaggtgatg
     5101 actcaagttt aatatttaca agtcagatat attataatta taaatataat tttaaattat
     5161 ttattccaaa taatttattt gataaaatac ttgttgaaca ggaatttgtt ggagaaaaag
     5221 cagtatttaa attttactat tttgactatt cagaccgtaa gaatttattt aatatagtag
     5281 tagagagtaa aaataaactt gaagatagaa aaaatacagg tactcaaaat tctattgtac
     5341 ttcaagagag cgatgaatat actttcttac tagttgtaaa caatgcaaat gaaatggata
     5401 agctagatat gactatagac gcattaaaag aatatttctc attaatatat ggttaatatt
     5461 atactcaatt ggaggtttta ttttatgaag gaaaagatac ttatactaga agatgaaatt
     5521 ggtattagaa gttttgttag tataaactta aaaagggaag gctatgaaat agttgaggca
     5581 ggtactggaa gagaagctat agaaaaaatg acaacagaaa aggatattac aatagctctt
     5641 ttagatgtaa tgcttccaga tataagtggt atagaagtat gtaaatttat aagggagaat
     5701 tttgaccaag taggtataat aatgcttact gctaaagctc aagaagatga taaaatagaa
     5761 ggatttatct caggagcaga tgattacatc ataaaaccat ttagtataaa agagctttta
     5821 gtcagagttt ctgctcttct tagaagagta gcaaaggatg attcaagtgt taagtcgagt
     5881 gagatagttt ctccaccatt tatacttgat atagacaaaa gaaaattatt taaaaatgga
     5941 aaggaaattg agcttacacc aactgaattt tcaatagtga aatatctaat atcaaatgct
     6001 aagcagtcgt taagtagaga ccagatatta gacgaagtat ggggaactaa ctatttatat
     6061 gattttaaaa tagtagatgt aaatataaga aggataagaa ataaaataga agatgaccca
     6121 tcaaaaccaa aatatataca aactatttgg ggttatggat actgttttag aaaggaagaa
     6181 taatagtgtt acattttgaa ggtctgagaa aaaaagtaat aaaaaactat tttataataa
     6241 ttattataat ggttactctg tttgaaggat tgttcatgtt ttacatacaa aactactatt
     6301 atgattctgt caaacagcta cttgaatctg aaataaaata tgctgatgaa tataatgcta
     6361 ttacaatgga aactacaagt tttgagaaaa aagtaaaaaa tatatttgat aaacaaccct
     6421 taactaaaaa ttcagaattt ggaatttcaa taatagataa agataaaaat attatattag
     6481 accaatatgg ttttaaaagt aaagaaaaag caaactatga agatgttaac aatgcattaa
     6541 aagatataaa aactaaaaat ttaacacctt atacttatag aataccagat acaggggagc
     6601 atgttatgag tatctcctta ccacttaagg taaataatat aatagaaggt gttgttagat
     6661 atacagtatc attagatgct attgataatg caatattaaa gcaagctaca tggcttatat
     6721 tagcaggaat atttatactt attatagcta tactaataag tcttaaattt gctgagactc
     6781 ttataaaacc attacgagag ctaaagaaat ttgcaaatga attggctgta ggaaattata
     6841 atataaaatt agaaaagatg aagattgtag atgatgaaat tggtgattta gcacagacat
     6901 ttgaacacat ggcacatgag attgacaaaa gtgaaaaatt aaaagaggaa tttatatcat
     6961 ctgtatctca tgaactaagg actcctctta catctataaa ggggtggagt gaaaccttag
     7021 gttatgaaag tataacaaga gaagaactag acttaggttt aggcattata caagatgaaa
     7081 cagaaagact tataaaacta gttgaagaat tattagactt ttcaaggctt tcttcagaca
     7141 ggataaaact tcatgttgat atagttgatg ttgaaggttt aattgtaggt gttgtaaatc
     7201 aacttaaagt taaagcagct gaaaaagata tatcactttt atttgaattt gaaaatgaat
     7261 ttatagagaa cattcaagga gataaaaata gattaagaca ggttctcata aatttaatac
     7321 aaaattcatt taaatttacg agtcaaggtg gttacataaa agttgtagct tctcaagatg
     7381 aagagataac aaccatatca gtagaggata atggttcagg tatagaaaag caaaatttaa
     7441 ataaagtgtt agataaattt ttccaagaag attataataa ggctggtagt ggattaggac
     7501 tagcaattag taatgaaatt gtaaaacttc atggtggaag aatgaaaata gaaagtgaaa
     7561 agaatgttgg aacaaaaata acttttaata taaaaaataa atttgctaaa caagcataaa
     7621 ttcttagaaa gaggattaag atgagaaaca taaaaatgat agtagcatac gatggttcaa
     7681 gatataaagg atatcaaaaa ttaggtgaca ataatatgac tattcaagaa aaacttgaaa
     7741 atgttttaag taaaatgact aatgaaacag tggagataat aggttctgga agaactgata
     7801 tgggagctca tgcaagagga caagtggtaa attttagaac taattgtatg gattctttgg
     7861 ataaaattca aaaatattta tatgaatatt taccagaaga tatagttgta aaaactgttg
     7921 aagaagttga tgaaagattt cattcaagat acaatgtaaa atcaaaaact tatatgtata
     7981 aaattgacaa taataagtat catagtcctt ttataagaaa atatgctact catgtaagta
     8041 aaaaattaga tttagataga atgagaaaag caagtgagta tttagtagga gaacatgatt
     8101 ttaccagttt tgcttcttct aaatctaaga aaaaatcaaa tgtaagagaa atttattcta
     8161 taaatataaa agaagatgat aatgttatag aaatatatgt agaaggaaat ggatttttgt
     8221 ataacatggt aaggataata gtaggtgctt taatagatgt tggtctaaaa agaagagctc
     8281 cacaagatat taagtatatg cttgaatcaa aagatagatg tcaatcatca gatactgcac
     8341 cagctaaagg actatgtctt tggaaagtaa catattaatc aattatataa aactctcttt
     8401 aaaataaaaa taaaataaat tatatagttg acataaatgg aaaaataaac tattatatat
     8461 tttataagag agatataaaa ctttatattt aaatcctgtg agaaagagag taacttctat
     8521 gtgttttata gagagctgag gatggtggaa gcttggcaaa taagtattag tgaaaagaac
     8581 tttggaggat actaattgaa tttagtatgt tagtaggtaa tctcgcctta agagaaagag
     8641 tggataagtt tatcttatca attagagtgg taacgcggat atatttcgtc tcttggtttt
     8701 tccaagaggc gttttttaat atatttaatt attagtgttt atctggtatg ttgaacattt
     8761 actaattgat tagctaagtt tatcaataag agtggtaccg cggatatttc gcctcttagt
     8821 attttaaaat actaaggggt ttttttagta ttaaaatgat tttatgtgag tctaaattct
     8881 cagagtcatt caatattaag aatttctata aaataaaaat tataatttaa gattgggagg
     8941 aattaaaaat gaaggaaaaa gtagtattag catattcagg aggacttgac acatcaatca
     9001 taataccttg gttaaaggaa aattatgaag atatagatgt tatagctgtt tgtggaaatg
     9061 taggtcagga agataaaatg gaagatgtat atgaaaaagc tttacaaagt ggagcatcaa
     9121 aggcatatgt ggatgatata agtgaagaat ttgtaactga aactatattt aaagccgtaa
     9181 aagcagaggc taaatatgaa ggaaaatact tattaggaac ttctttagca agacctataa
     9241 tagctaaaaa attagttgaa gttgctcata aagaaggtgc aaaatatata tgccatggat
     9301 gtacaggaaa aggtaatgac caagttagat ttgaagctac aattgcagct cttgacccga
     9361 ctataaaagt tattgcacct tggagaattt gggatataaa atcaagagaa gatgctatag
     9421 actatgctga aaaacataat ataaaggtaa cagcaactaa agctaaaata tattcagtag
     9481 atgctaacct ttggcatgtt tcaacagaag gtggagatat agagcaccta gaaaatgaac
     9541 ataaaaaaga tgtttataaa caatgtgtag acccagaaga tgcatgtgat gtagctgaat
     9601 atgtagaagt ttactttgaa aaaggtgtac ctaaaaaggt aaatggtgaa gaattatctc
     9661 cagttgcact gattcataag ttaaatgagt tgggttgtaa acatggaatt ggtgttatag
     9721 atatagttga gaatagactt gttggtatga aatcaagagg tatatatgag actccaggag
     9781 gaacaatcct ttatgaagca cataatattt tagaaagtgc tactttagac aaagatacat
     9841 tacatttcaa acaaatggta tcttataaat acggagaact tatttacaat ggactatggt
     9901 attgtaaatt aagagaatct atagatgcat ttatggaaca aactcaagat aatgttacag
     9961 gaacagtaaa agttaaactt tacaaaggaa atataaaacc tgctggaata tttactgaaa
    10021 atgctcttta tgatgaagga atatcttctt ttggaaatag tgaactttat gaccacaaag
    10081 atgcagaagg atttattaat ttattcactt taccattaaa aatcagagct atgaaagctg
    10141 gaaaataata aaataaatta tttaagagtg tataaaaact atttttaggt ttttatatgc
    10201 tcttttttta tttgaattta ttaacttata cagtaaatag tataaaaaaa tatattatag
    10261 ttccaaatgg cttaataatg tgaaaatata agacctaaaa tataaatttt ataaatacaa
    10321 tagttattga ctctctcatt gtaagagggt ttatactgaa tctaatgaaa atttatagtg
    10381 aggagataga ttatatgaga aaaataattg aaattaaaca tcattcacat gattatgagt
    10441 gtatgtggaa tggtatagaa gatttatata taaataaaac cggtgaatgc cttccaaacg
    10501 attttttctt tttactgtca ggttttggtt cattttgtta tatgaaaaca aataaagctg
    10561 atttgaaaag aatggtagct ttgggagatg gaaggacaaa acagatgtat aaatttttag
    10621 caccaattgt tggatttgaa tacaaacatc atgagtttaa aaagtatgag caagcattaa
    10681 agaaagcaaa atctgaaatt gatgatggat atccagttat tttaggagct ttagatatgt
    10741 attatctacc atattataag aagctatatc ataaagaaca tattcctttt cattatatat
    10801 taatggtagg atatgatgat gaaggaatat atctgtatga ttgtggtaga acagaattat
    10861 tacatctttc ttatgataaa ttacgtgaaa gcatgaattg caattcccct ggattaagca
    10921 aagaaaatac tatttgcaca ataagaatga atacttcaaa gaataaatat aacattgcta
    10981 gagaagctat tgctataaag cgtgatatgt ttttaaatcc acctaaagga tttttgggat
    11041 ataaaggttt tgaaaagttt attaaagagt tgccacagtg gaacactgaa ttggataaag
    11101 atgattatga taagattttg accaatatgg ttatgttttt tggaacagta ccaacaattc
    11161 caaatgcaat caaaggaatt aaagaaccag atactgttca atttaaaggt ggatttgata
    11221 aaatgagtag aatgctcaac agtattgggg aggaatacaa aaacgactct tttatacaag
    11281 tagctgatat ttttgataaa actgctatta taattgagaa aatatccaat attataatag
    11341 attacttaac tcaaacatgt gatgatacag gacaacttcc gttattgttc tctgaggtct
    11401 tagagcatat gaaaacagga tatttaatct tggatgtata aaaaatatat tgttgaccct
    11461 aacattacgt aatagtgtaa tatgaaaata tagcttgaat atattagtag gtattgatgg
    11521 ctgtaaaatt aaaatttgag gtgtggatat gagaacagtg aaacaagttt ctgatttaac
    11581 tggaataagt gtgagggcat tacattatta tgatgaaata ggtcttttaa aaccaaataa
    11641 aattacagat gcaggatata gactttatga tgatgaatct attaaaactc ttcaacaaat
    11701 tttatttttt aaggagattg atattccttt aagagaggtt aaggaaataa tgtcaagcca
    11761 atattttgat aaggttgaag ctctaaaaaa tcaaaaaaaa ttacttattt taaaacgaaa
    11821 gagactggat gaattgatag agcttataaa tcaaactcta agaggagaag gaaatattga
    11881 ttttaaagag tttgatatga gcgagtattt tgatgtttta gaagaattta aaagagagca
    11941 taggaataag gtaattaaaa tttatggtag tgtagaaaaa tacaatgaat atatagaaag
    12001 agttaagtct aatgaagaga aaattgctaa aatggctata aaacaatatg gaactattga
    12061 gaagtttgct aaagctataa aaacaaattt tagcagtgat atattgaatt taggagaaaa
    12121 atttgataga tataaaaatg attgtttaaa agaaaaacat ccaaaattaa aagaattata
    12181 tagaaagctt gtagaagatt taaataggaa tccttcttca acagaccttc aagaaatagc
    12241 aaaagagata acagatatat ctaaaaaaga ttatgaaatt ttctctatgg atactggtga
    12301 tgacaactgg tattatatgg ttcaaaatta tctagtaaat cctatgtgga tagaagaagt
    12361 agataaaaag tatggaagtg gagcaggtaa gtttattgga caagtactaa agacttatct
    12421 aagagataga aaaccaaaga taaatacatt gtatgaaaaa cttgtagaag atttgagtag
    12481 agattgttct tcaagggaag ttcaaagcat tgttgaagaa atagacaatg aaatgaaaag
    12541 aagtaatgaa ttttataaaa tagacaatgg tgaaagatac tttgattata tgtcagagct
    12601 ttatttacaa gattctaact atataaaagt aactgataag aattatggtg atggtgcatc
    12661 taagtttatt ggagaagctt ttaagattta ttttgataat aataattgtt gaaaatttgg
    12721 aagtaaaaaa tatgcaagtt ttataaaagc acaaaaataa ggatgtagat tagctccatc
    12781 tatatcctta tttaaagtta ttaaaatata tagtttacat caaacttaac actttcaata
    12841 atatttccca aatatgtttt tatactttca acttcttcta aagattttaa tttattttca
    12901 gtataaattg ctttgtccag aatattaaac cttaatccta tttcatcaaa gtactcatga
    12961 tttaaaactt tgtaccaatg gtttttttct ttattccaac tcgtagatat tgaatcagct
    13021 tctttttgag ctttttccca atcttcattt tctatagatt tttctaaaac agatatattg
    13081 tctttatatc cttctgtaaa atcatatagt ttattgttaa cataaaatcc aaataataca
    13141 aataagatag tccatagtat aacaaatgtt gtagatttca atattaatca ccctttttat
    13201 tttgaataaa tattttgtca ttttcatcta aaacacaaat aagaacatct ttaaaagttt
    13261 ctatgtgatt agattttaaa atacccatta accagttagt atctttttga gctatgtcta
    13321 gatttttatt tattattcta ccatccagta taagtgggat aggtaaatga ttaaaagctc
    13381 ttggaggagt actattgtag cttgatgctg gtacaacact tagattacca tcagtctcca
    13441 aaatagcata ttgtacatct tttaggttaa agtatccttg aatcctaagt tgttctaata
    13501 attcatctat actaattctt tcttttttaa gctctttata tacaattttt cctttatcta
    13561 ttaatacaga aggtttacct gataaaaatg aacttaattt tctacttttt aatgataaga
    13621 atgaaattag agtctgcatg attacaagtc cagatattgc tgcaacacca tttatgattg
    13681 gtatattatt attttccatt gggacagatg caacttctgc aattaatagt gttataacta
    13741 aatcaaaaca gttcatttca gctatttcac cttttcccat gactctaagt gcaattagca
    13801 ctgttatgta taatataata cttcttatta atactactgt catatctatc aattactcct
    13861 ttaaaaaata tttacactta gttagtatat cctatataaa aaatttaatt caaagtataa
    13921 aaaaccttaa ataattggat tcaactattt aaggtttttt tgataatttt aatattaaat
    13981 tttttatgga ttttctgtta cttttattta ttttaataac agattcaaca aaactctttt
    14041 tatctcctaa ttttaagata aaatcaggat attcttcaaa ttcattgagt tttattaatt
    14101 tgaaagaaga agatagattt ttaaagttaa taattttaga gttattcact aatacacttc
    14161 tttttttaaa ataaatttct tttttactta tatttagtat agttaatatt tgtttaagat
    14221 atattattat atcttctgga gttctatcaa atttgagttc aaaattttca tcttcaaagt
    14281 agggaatata atataaaggt gttttgagag caggtaattt attatataat tcttcaagaa
    14341 tatcgatatt tttaggggag ttaaagaaaa agcatttaca tacattcatt aaatccacag
    14401 catctgaaag tgagttatgt ttaatatctc cttctacaga aaaaattttt ttcatattca
    14461 gtaaagatat ttgcttagat aagattctat tattgtatgt aatatgttga gatataaaag
    14521 gttgaatgtc aatcatcata ttaaataact ctaagcattt acctgtatat ttatatactc
    14581 taaatgaagt tagaaggcca gttatatcaa aatcacccca attatatatt gtaacatcat
    14641 caaatttaca aaaccattta taaaaatcat ccataacata taaaagatta ggagcattat
    14701 tgatatctag ttggttcaat ttggttaaat ttttacattt atttgaaagt attttattgt
    14761 attttggctt aactaaagag taataattat ctattgtatt aaattcgtta tcaactaaaa
    14821 ctccaccaat agaaattaat tccgcaccat tatattcttt tggtttattt ttattatcat
    14881 ctagtatatt aaattcaaag tctataaata acttgtattt cataaatcta aagcacatcc
    14941 tcaggtaaat tatttattga gcctaagcat cataagaatc aatagatata atgactctaa
    15001 acatcaaagg ttcattacaa cttttattta tttcataatt taattcaaat aaatctttta
    15061 agatataatt tataagtatt ctttcccaac ccatttttaa attaacttta gagtctatta
    15121 atatattttc tatcttttta aatatttcta tatttgctgg tattatactg agctcaatat
    15181 tacttttaaa cgggttagac tgtaaatctg ttataagtat attttttaat tcctctgtaa
    15241 gttcagaaat taaactatca cctatgtatt tttttatgat ttcctctcta atttctttta
    15301 aatttagcaa tattagtacc tgctacttac atagtagctt ttcctttcct tttaatacta
    15361 ttattcattt ttgaatctta tttagatata ttttaagatt aaaagtcttt agctatataa
    15421 ttttttaccc atttttaact tatattaaca tattacaggc ttatatctcc accctgttta
    15481 cattgaggat ggagtatatt gcctattttg ataaaaatga taggttgcta tttgcctatt
    15541 cctttttctg ataatttttc taacatatct ttggtcattt tatctaaatc ataactaaac
    15601 ttaaagttcc attcttttac tgcactacta ctgtctagag agttaggcca agaatcagct
    15661 atactttgtc ttacagggtc tacatcatat tctatcacaa aatcaggaat atattttttt
    15721 atagaatcag ctatttcctc aggagaaaag ctcatagcag taatattaaa agcatttcta
    15781 tggatgagtt tatcagctgg agcttcgatt aaatccacta ttgattgaag tgcatcaggc
    15841 atatacatca tatccatttt tgtaccttct gctatgtaag atttatatct cttatttttt
    15901 aatgcctcat aatatatatc aactgcataa tctgtagttc ctccaccagg aggcgtaaca
    15961 taagaaataa gacctggaaa tctaacacct ctagtgtcta ctccaaattt tgagtgataa
    16021 taatcacata gtaattctcc agaaacttta gtcacaccat acatagtatt aggtctttgt
    16081 aatgtatctt gaggagtcat attttttgga gaattttcac caaaagcagc aattgaacta
    16141 ggtgtaaaaa atttacaatc caattctttg gctatttcaa gaccatttat taatccattc
    16201 atatttaagt tccaagcttc tagaggttta ctttcagcaa ctgcagaaag taaagatgct
    16261 agatgaatta tagtatctac atcatatttt tttgcaattt caaaaaaacg attcttatct
    16321 gtaacatcta atatctcaaa tataccagat tcacatactg gattaccttc ttttactctc
    16381 cttgaactag cgattacatt ttgttctcca tactcatttc taagctttat tgttaattct
    16441 gaacctattt gtcctaatgc acctgttata agtatttttt tcaattttac aacctcctaa
    16501 gggtttaatt tatgtagtac tacttatttt tattttagca aatcttttgt ttaaatacaa
    16561 agaattaaag ataataatat acgacttaat tgaatgaagt gcagtaagtg cctaaaaatt
    16621 taaagctaat atataatggt atattgtata acaataaaag agtagattaa attattatat
    16681 atagattgtg tgactaagtc acagaaaata gagttaattt aatatataat aaaagcatat
    16741 caagtaaagc tttaaattaa agtaacttta taaataaaaa gtaatataaa ttgataaaat
    16801 tataaattta tttatagtga atatgggttg gattttatag atacaaaaat agtatatatg
    16861 atatgtagac cattagcagg tttaaagtag caaaacatat aaaataaaat ttgaaaggaa
    16921 gtataaaaat gacaagacca tcaatatatc atagccaagg atacacttgt gcagaggcat
    16981 taataaagtc ttataatgag gaacataaca cagatatacc aatctcaata ggaagtggta
    17041 tgggggttgg aatgaatgtt ggaagtgttt gtggagctgt aaatgctgct gttgttataa
    17101 tagggtattt aaatgggcga aatagtaatt tagatgaaaa tatagcaagg aagtattcaa
    17161 gagagctatt gaatagagta agaaataaat acagctcaga aatatgtgta tctcttaaaa
    17221 aaaataaagt aacttgtgaa gaaataataa atttcacata tgatgctcta aatgatatgc
    17281 taaaaaatca aaaataattc taagaaacaa gtattttaca ttcgatagaa tacttgtttt
    17341 tttatgatat ttttaataag tattagttta ttataatttt taaatcaatt ttattatatt
    17401 ttaattgaaa caatctactt tgaaaattac taactgacaa aagaat
//
LOCUS       DAMJVK010000085        17335 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.086, whole genome shotgun sequence.
ACCESSION   DAMJVK010000085 DAMJVK010000000
VERSION     DAMJVK010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17335
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.086"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            115..2178
                     /locus_tag="PC414_002847"
     CDS             115..2178
                     /locus_tag="PC414_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2465232.1"
                     /translation="MTKIILSNYRCFGEEPTTIEINNLTGFIGHNSSGKTALLSALMK
                     LFGEKSSDRIIERSDFHIPESIDPDSISKNELYIEAQFEFPELERGKDSISIPVFFKN
                     MVVSEPGGKPYLRIRLEANWEKSSNPEGSIDSNVYYITSTTEEINPEDKRNIKRYELD
                     QIKMIYVPAIRRPDEQLKNVSGTIISRLLNGISWSEQTKARIKTKASETEEIFFEEKG
                     VESLKKSLETQWNSYHKDIRYSNPKINFNSTDLETILKKVEVTFSPTGTHRDYRVEEL
                     GDGLRSLFYLSLVNTFLEIEEEILKSINDGKEEDIFTLIPPVLTIVAVEEPENHISPH
                     LLGRVVEKLNFISTKENSQTILTSHSPAIVKRIEPTDIRYFRICKEYECTKVKSIILP
                     NEEKEAEQFKFVKESVLAYPELYFAKLVVLGEGDSEEIIIKQMIELKAESVDSSEISI
                     VPLGGRHVNHFWRLLNDLEIPFITLLDLDRERDGGGWGRIKYAIQQLINIGHDKNVLL
                     YTCEGISQDNEFKEMHTWDVTDTENMAKWIEMLKGYDVYFSAPLDIDFAMLQSFKDKY
                     ISALTSAEGPRIKDHGKIQDIVINEDAEDESLKAAYKDRIDSDIKSTLKQEGGDGSTY
                     TEGEKELMIWYSYFFLGRGKPTTHLSLFSNNTDIDFESFPYCLKEFVNSIIEKMIDDS
                     GGGRK"
     gene            2175..4040
                     /locus_tag="PC414_002848"
     CDS             2175..4040
                     /locus_tag="PC414_002848"
                     /inference="COORDINATES: protein
                     motif:HMM:NF024642.1,HMM:NF024754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDF2465233.1"
                     /translation="MKRNWVSKEDWSPAAGFRLEVEAMNTVINDKNTLVVAGPGAGKT
                     ELLAQRACFLLETNTCRYPKKILAISFKKDAAENLKERLELRCGKELASRFESKTYDA
                     FAKEIVDRFRNSLDEAYRPLNNYNIAENRDVRRAFEIAGLNFRGNQTEFNKVYGNKLS
                     INKLPLHTDFVDKIFIDAWNVLLKGNKDTNFVSSLTFGMISRLAEYLIRTNRYIKKAI
                     EMTYSHVFLDEFQDTKSIQYDLVKACFFKTETIITAVGDGKQRIMLWAGARKSIFEDF
                     QNDFLSQKRTLIMNHRSAPRLVEIQKAMYNSLNEEAIDLKTNEKWNYEDGEVYLRLFE
                     DYIKEADILSEEIVELVETGIEINEICILVKQSVDIYSKEIINKLSERNIKARNEAMY
                     QEILKEDIIKTLVSLFRLSLSDRNPDDWDYIYDILGELYGIDEGYNPKILNDFITKVE
                     LMINDLKVNLNITDEKQFSDLVDSKVEYISYEKFASKFQKYKSKDYFDDLVDKFKKLV
                     WIEYNETKDWIKAIDNFEGKNSIPIMTIHKSKGLEYDTIFFVGLEDSAFWNFKNQPDE
                     DRCAFFVALSRAKRRIDFTFSLDRPAGFSNSQKHYCINEFYELFDNTEIVKKIEY"
     gene            4164..4978
                     /locus_tag="PC414_002849"
                     /pseudo
     CDS             4164..4978
                     /locus_tag="PC414_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728762.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5003..5491
                     /locus_tag="PC414_002850"
     CDS             5003..5491
                     /locus_tag="PC414_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDF2465234.1"
                     /translation="MYEKDYKQYIKLDIPIDEAKIKNRFEIRLKNERAYHAAVDLLAN
                     RDEEKTAFSIINRYIRFVDKDDEKIRSEWKTNDQWSYFIGEGRAKLKLTTNPEPYNIQ
                     KTLNWLSRQVAPTLKTIKRLDSLNNSTLIEGLIRYARLTDKHKKIIEQNSVNVEDIIY
                     DE"
     gene            6005..6232
                     /locus_tag="PC414_002851"
     CDS             6005..6232
                     /locus_tag="PC414_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2465235.1"
                     /translation="MDIVKVFANNVRKYRNKKGLSQEAFAEKAGLHRTYICAIERKKR
                     SIALDNVQKIADALEIETYLLFIEGEEKECL"
     gene            6229..9168
                     /locus_tag="PC414_002852"
     CDS             6229..9168
                     /locus_tag="PC414_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HDF2465236.1"
                     /translation="MKKLPIEKLIENLGFSGSKNLVNIDMLTKLDISKHDVKTLKVIN
                     PYAIYLVNSKPFILFIESSFEEKKLQQISKLIWNAQIPIAFICDSNSVKIFNGKALDL
                     KSYLIEEIYNQSIEDFSEESEFSYLKISDPLFWDQYTKQYTKTHLNDYLLDNITSLTN
                     KLKKKYKVKFATKLVLRLIFIRYLIDRGVDIAYKNFNHDIKASQKELLKITKNKVELY
                     ELFKYLKEKFNGNLFELNDEINNTCLTDEVFILLSDFLSGKISIDNGQMSLFSMYDFN
                     IIPVELISNIYEILLGKEIRNKDNAFYTPNYLAEYILDKTTLEFLKNNRQYKILDPSC
                     GSGVFLVNSFRRIIDMNLGDKLFCEDDKLLKELLLSNIYGIDINEDAIDVTIFSLYLT
                     ILDYKDPKILSEFKLPNLKGCNLIASDFFDDNKLESLKKINFDFIIGNPPWGSVSDGL
                     HLDYCNKNGYNDMQQNKEISRSFIFRAKDFSSRNTVCSFVVHSKILYTQKKPSKKFRE
                     FLLKKSQIFSVVEMSSVRKLVFKNADAPAAVISFKYNESEENLNNRIIYTSLKPNVFF
                     KLFNIIVIEKYDVKYISQKLLLENDWAWKTIVYGFSKDIDIIMNIKKKYQTVNEIILK
                     NSFVHGTGIQINGNESHDATHLKGKWYVDSKKGIDSFYVDINKGKELTQDKMRRPKAN
                     QQEIFIPPYVLLKKGVDTKTYRYRAAFSNDAFVYSDAVTGICGKKDNLTTLYCLTGML
                     NSSFYSYLNIMLGTSIGIEREQGFSTEIFTYPAIESDEIANLVVKIQEATKLEKTTMF
                     LNEKNSCKYIKQLDKLILKSFNLNDDKFIDYAINIQIPELTNSKSTNIYRKITKEDLI
                     NYSECFKEQFSSIYRRVGKSISISVYPKILNRFTVFELEVTDEKTDREIMVKAEADNN
                     MELFTKIIRYDYNDVFHQIRDIIHFGENSFYIIKPNCYKYWHPAIAEMDLADVLDQIL
                     SDTGGEE"
     gene            9168..9842
                     /locus_tag="PC414_002853"
     CDS             9168..9842
                     /locus_tag="PC414_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465237.1"
                     /translation="MGGFKNSSLNFYLKQGLYEIIIDGLIDCCLLMSKSCIEQSRKIL
                     NHEEKIRTHLVENYLENDIERRKTSLSKQHLRFEVEAQENYDNKNESYEGRVDIKVIS
                     KDTLADRRAYYIIECKRIDGKETLNKKYITEGVQRFITEKYSSHYGKNIMLGFIVKDI
                     NIQSNIQKINSLNQNILYTYIDTDMLTVRSDSENNYAVCESKYKVKSKLLLLNHIFYN
                     FSSIIR"
     gene            10003..10419
                     /locus_tag="PC414_002854"
                     /pseudo
     CDS             10003..10419
                     /locus_tag="PC414_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455547.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            10379..12826
                     /locus_tag="PC414_002855"
                     /pseudo
     CDS             10379..12826
                     /locus_tag="PC414_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005423199.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            12966..13388
                     /locus_tag="PC414_002856"
     CDS             12966..13388
                     /locus_tag="PC414_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465238.1"
                     /translation="MRLEDIIDKESYEEKYDDLTVEIKKLSKEKKTLKASLINEKSIK
                     ARLREFRKILERNELLEKFDRYVFASIVDKVIVGVINEDGIVDPYKITFVYKTSFDDK
                     QDGHKFINKSPLETYSKDNNDNKKKCSYTNNETLRDCS"
     gene            13926..14960
                     /locus_tag="PC414_002857"
     CDS             13926..14960
                     /locus_tag="PC414_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH domain-containing protein"
                     /protein_id="HDF2465239.1"
                     /translation="MFKKKLIAVAMSITMISVGSFSYAHSGRTDSSGGHRDNKNKSGL
                     GSYHYHCGGYPAHLHNNGGCPYTGGGSSSGTTTSVNNEEKQKRSVGEKGYNQGYEDGY
                     KGNYSSSNYSGDYSDTYESKYSEGYEKGKAKLEEEEKVAKETGYNLGLTGAKSNNTYE
                     KEVLKNAYDTGYSTGYNEYKTKKIEEYKAKGIEDSNKDKEKMTFEENIDSEFIDAYNN
                     AYDEIQEQLKNDYTTQGFESAIKGERFDTSTIGNVKYANWFKEGYDNGKVKLPKAKES
                     VYNQGYNEEDFSIPDEMKSIETKLKGVYDEGLEKREEEKSRNATYGVGAGTAAVVAGV
                     VYKKKKRKKI"
     gene            15047..15250
                     /locus_tag="PC414_002858"
     CDS             15047..15250
                     /locus_tag="PC414_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HDF2465240.1"
                     /translation="MGFNFRKSINLGGGLKLNLGKKSVGISAGVKGARVSVNSKGRKS
                     ATLSIPGTGISYTKTSTKKRKPK"
     gene            15421..16581
                     /locus_tag="PC414_002859"
     CDS             15421..16581
                     /locus_tag="PC414_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp27"
                     /protein_id="HDF2465241.1"
                     /translation="MKLSKILLAIGMSVSLLIASCPTSNALSSINSIKGTNKYETAGL
                     IADKQNYDIAILINSENGLSDGLSASGLSGAKNAPILLTKKDTIPSETLKRLEKVKEV
                     YLIGGKNSIGASVQKILSDKKISINRISGTDRIDTSHKVAKEIGRVSTVKKVILTNAF
                     KGEPDAMSVASIAVRDKSPIVLTDGNTTSFATDGLETYAIGGKVSMSDKLINETKATR
                     IGGVDRYDTNKQIINKFYNGSKEFYIASGTDLVYSLVGSTIAQKTPIVLVGNGSNKSI
                     LKNASKITAIGNIDDVVIKQCLNITNNVGDSNTGIVKDTNSNNTNKPSNEPSSNNPGL
                     TDKSTVYVTPNGKSYHLTKNCSTLKRSKTINKVTLKQAKSQGKIDPCNVCVK"
     gene            16840..16980
                     /locus_tag="PC414_002860"
     CDS             16840..16980
                     /locus_tag="PC414_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465242.1"
                     /translation="MENLMMSIITGVIASYIYDKLKSHSERPTKSGWELNIKFQKSKS
                     SK"
BASE COUNT         7098 a         1680 c         3006 g         5551 t
ORIGIN      
        1 tattcgtata ataatagaaa tattttagca taagatgtat aataaaggaa aaaatacaga
       61 attaaaaaac ataagataga taagtggact taggggagag aaattattat gcaattgaca
      121 aaaataattc ttagtaacta taggtgtttt ggagaagaac ctacaactat tgaaattaat
      181 aatttaactg gatttatagg tcataatagc agtggtaaaa ctgctttatt aagtgcactt
      241 atgaaacttt ttggagaaaa gtctagtgat agaattattg aaagatcaga tttccatata
      301 cctgaaagta ttgatccaga tagtatttct aagaatgaat tatatattga agcacaattt
      361 gagtttcctg aattggagag aggtaaagac agcatatcta taccagtatt ttttaaaaat
      421 atggttgtta gtgaaccagg tgggaaacct tatttaagaa ttagacttga agcaaattgg
      481 gagaaaagct caaatcctga aggaagtatt gattcaaatg tatactatat aacatctaca
      541 acagaggaga taaatcctga agataagcgt aatatcaaaa gatatgagct tgatcaaata
      601 aagatgattt atgtacctgc cattaggaga ccagatgaac agttaaaaaa tgtctcagga
      661 acaataattt caagattatt aaatggtata agttggtcag aacaaacaaa ggcaagaatt
      721 aaaacgaaag caagtgaaac ggaggaaatt ttctttgaag aaaaaggggt tgaatcatta
      781 aagaaatcac tcgaaactca atggaatagt tatcataagg atattagata ttctaatcca
      841 aagataaact tcaatagtac tgatttagag acaattttaa aaaaagttga agtaactttt
      901 tcacctacag ggacacatcg agattatcgg gttgaagagc ttggagatgg gttacgttct
      961 ttattttatc tttcacttgt aaatacattt ttagaaattg aagaagaaat tctaaaatct
     1021 attaatgatg gtaaagaaga agatatattt acattaatac cgcctgtatt aacaattgtt
     1081 gcggttgaag aaccagaaaa tcatatttca cctcatcttt taggaagggt agttgaaaaa
     1141 ttaaatttta tttctacaaa agaaaattca cagacaatat tgacatcaca ttcaccagca
     1201 atagtaaaaa gaattgaacc aacagacata aggtatttta ggatttgtaa ggaatatgaa
     1261 tgtacaaaag tgaagtcaat tatattacca aatgaagaaa aggaagcaga acaatttaaa
     1321 tttgtaaagg aatcagtatt agcatatcct gaactttatt ttgcaaaact tgttgtattg
     1381 ggcgaaggtg atagtgaaga gatcattatt aagcagatga ttgaattaaa agcagaatct
     1441 gtagattcat cagaaatttc aatagtacca ttaggtggta ggcatgttaa tcatttttgg
     1501 cgtttattaa atgatttaga aattccattt attactttgt tagatttaga tagagaacga
     1561 gatggaggtg gttggggacg tataaagtat gcaatacaac aattaataaa tattggtcat
     1621 gataagaatg tattacttta tacatgtgaa ggaatttcac aagataatga atttaaagag
     1681 atgcatactt gggatgtaac tgatactgaa aatatggcta aatggattga aatgctaaaa
     1741 ggttatgatg tttatttttc agctccgtta gatatagatt ttgcaatgtt acaaagtttt
     1801 aaagataaat atatttcagc attaacaagt gcggaaggtc ctaggataaa agatcatgga
     1861 aaaatacaag atattgtgat caatgaagat gctgaagatg aatcattgaa agctgcttat
     1921 aaagatagaa tagatagtga tattaaatca accttaaaac aagaaggtgg tgatggatct
     1981 acttatactg agggagagaa agaactaatg atatggtatt catatttctt tttaggaaga
     2041 ggtaaaccaa ctactcattt atctttattt tctaataata ctgatataga ttttgaaagt
     2101 tttccatact gtctaaagga gtttgtaaat agtattattg aaaagatgat agatgattca
     2161 gggggtggaa gaaaatgaag aggaattggg ttagtaaaga agattggagt ccagcggctg
     2221 gattcagact agaagttgaa gcgatgaata cagttattaa tgataagaat acacttgttg
     2281 tagctggtcc tggcgctgga aaaacagagt tattagctca gagagcctgc tttttactag
     2341 agactaatac ctgtagatat cctaaaaaaa tccttgctat tagttttaaa aaggatgctg
     2401 ctgaaaattt aaaagagcgt ttagaattga gatgtgggaa agaattagct tcaagatttg
     2461 aatcaaagac ctatgatgct tttgcaaaag aaatcgtaga tagatttaga aatagtttag
     2521 atgaagcata tagaccttta aataattaca atatagctga aaatcgtgat gttagaagag
     2581 catttgagat agcaggatta aatttcagag gaaatcaaac tgaatttaat aaagtatatg
     2641 gaaataaact atcgataaat aaattgcctt tacatacaga ttttgtagat aagattttta
     2701 tagatgcttg gaatgtacta cttaaaggca acaaagatac taattttgta agttcactta
     2761 cttttggtat gatatcacgt ttagccgaat atttgataag aaccaatagg tatattaaga
     2821 aagcaattga aatgacttat agtcatgtgt ttttagatga gtttcaagat actaaatcta
     2881 ttcaatatga tcttgttaaa gcttgttttt ttaaaacaga aacaataatt actgctgtag
     2941 gggatggcaa acaacgaatt atgttatggg ctggtgctag aaagtcaata tttgaagatt
     3001 ttcaaaatga ttttttaagt caaaaaagaa cattaattat gaatcatcgt tcagcgccaa
     3061 gacttgtaga gattcaaaaa gcaatgtata acagtttgaa tgaagaggca attgatttaa
     3121 aaactaatga aaagtggaat tatgaagatg gagaagtgta tttaagactt tttgaggatt
     3181 atataaaaga agctgatata cttagtgagg aaatagtcga attggtggaa acaggtatag
     3241 aaattaatga aatatgtata ttagtaaaac agagcgtaga tatttattca aaagagatta
     3301 taaataaact ttctgaacgt aatattaagg cgcgtaatga agcaatgtat caggagatat
     3361 taaaagaaga tataataaag acactggttt cgctttttag gttgtcgtta agtgatagaa
     3421 atcccgatga ttgggattat atttacgata ttctaggtga attatatgga atagacgaag
     3481 ggtataaccc taaaatatta aatgatttca ttaccaaagt agaattaatg attaatgatc
     3541 tcaaggtaaa tttaaatatt actgatgaaa aacaattttc agatttagtt gattctaagg
     3601 ttgaatatat ttcatatgaa aaatttgcat caaagtttca aaaatataaa tctaaagatt
     3661 attttgatga tttggtagat aaatttaaaa agctggtatg gattgaatat aatgaaacta
     3721 aagattggat taaagcgatt gataactttg aaggtaaaaa ttcaattcca ataatgacaa
     3781 ttcataaaag taaaggactt gaatatgata ctatcttttt tgttgggtta gaagattcag
     3841 cattttggaa ttttaaaaat caaccagatg aagatagatg tgcatttttt gttgcattat
     3901 caagagctaa aagaagaatt gattttacat ttagcttaga tagaccagca ggattttcga
     3961 attcacagaa gcattattgt attaatgaat tctacgaatt gtttgataat actgagattg
     4021 taaaaaagat agagtattga ttaagatgat aaaaacttta gtatattata ttttaagata
     4081 ttagttatat aagtagacat tgaatttatg tatttgatgt ctatttttta tataaaaata
     4141 tattaaagaa aggatgaatt tgcatgaaga aaaaaacaaa aatatctaaa aagattgtaa
     4201 gttttattat ggtttttata actatagtta ctactatact tacatcatca gtttcagctt
     4261 atgctagcga gttggatatg agtgaacaga caggctttaa ttatttaggt attagtccaa
     4321 ttacaggaaa aaaattaatc ataacattta tcgtatgaag atggataata aaattgtatt
     4381 ttgtgtagag ccaggaatta aaacttcaag tggaggagga tatatatcgg aggagttagg
     4441 agataaattt atatctacaa cacttccaaa ttatcataaa agaaaagctg aaatacttga
     4501 gaaggtaaat agacataata tattaccttc atggaataat gaaaaagttg cagttaaaag
     4561 tggagaaaca attgagttag aagataagaa tggtattatt agagaaatgc aattagaatc
     4621 aaataatact ggaacagata tattacttga agaaaatact ttgaaaatta cagcaaataa
     4681 aaattctaat tcaggagtta ttagttatat aaagattcct aaaaatgagg ttggaatatc
     4741 aatagtttat cgtaagcata actatcaaag cttatcagaa tttcatatgg aaagttcgat
     4801 gagtgcaaat ttaaatataa atgtaatcaa gttaggaatt gttaaagtaa aaaagattga
     4861 tgaaggcaca ggaaaggaat tagcaaatgc aaaacttaga tttgagtatg atggtgatag
     4921 taaagaaatt ataacgggtt ctaatgaaat tgctagtata gaagatatta ttggttgatt
     4981 aaaaagtgaa gtatactttt gtatctatga aaaagactat aagcaatata taaaattaga
     5041 tatacctatt gatgaagcaa aaataaaaaa tagatttgaa ataaggttaa aaaatgaaag
     5101 agcatatcat gcagcagttg atttgttagc aaatagagat gaagaaaaga cagcattttc
     5161 aattattaat cgttatatac gttttgttga taaagatgat gaaaaaatta gaagtgaatg
     5221 gaaaactaat gatcagtgga gttattttat tggtgaaggt agagcgaagt taaaactaac
     5281 aacaaatcca gaaccatata atattcaaaa aactttaaat tggttatcaa gacaagttgc
     5341 accaacgtta aaaacaatta aaaggttaga tagtttgaac aactcaacat taattgaggg
     5401 cttaataagg tatgcaagac taacagataa acataaaaaa ataattgaac aaaattcagt
     5461 aaatgttgaa gacattattt atgatgaata aaaaaatgta attagtattg cagattttaa
     5521 tataaaagaa gggagatata cgaatgaatt ttggtcaaaa tttatataat tagtttttat
     5581 caaatgcaca atcattggta ttaatggcta tagctataat tggtgtatat ttaggatttt
     5641 agagagagtt ttcaaaactt ataggatttt tagtagttgc attaattgca gtagggcttg
     5701 tgtttaatac agcaggagtt aaagatatat tattggagct atttaataaa ataataggtg
     5761 tgtaaggatt aaaagagaat agaaaattat gagtatatga tgaagggttc taataagttt
     5821 tattaaaata tatattgaaa gacaagtaga gctatttcga gttattacat gtaatgctaa
     5881 tatatataac tgcattttgg aaattaaata cataattaga aatagcaaat tttataggtg
     5941 acttattaat atgtttactt attgtatact atttgatata tttaatatga ggtgattctt
     6001 gaaaatggac atagtgaaag tttttgctaa caatgtgaga aaatatagaa ataaaaaagg
     6061 tctatcacaa gaagcttttg ctgaaaaagc tggattgcat agaacttata tttgtgctat
     6121 tgaaagaaaa aagagaagta ttgctttaga taatgtgcag aaaattgctg atgcgcttga
     6181 gattgagact tacttactat ttatagaagg tgaggagaaa gaatgtctat gaaaaaatta
     6241 ccaatagaaa agcttataga aaatttaggt ttttcagggt caaagaatct tgtgaatata
     6301 gatatgctta caaaattaga tatatctaaa catgatgtaa aaactttaaa agttataaat
     6361 ccttatgcaa tttatttagt aaatagtaag ccatttatat tatttataga gtcttctttt
     6421 gaagaaaaaa aattacaaca aattagtaag cttatatgga atgctcaaat accaattgct
     6481 tttatatgcg attctaattc tgttaaaata tttaatggaa aagctttgga tttaaagtca
     6541 tatttaattg aggaaatata taatcaatct attgaggatt tttcggaaga atctgagttt
     6601 tcttatttga aaatatctga tccattattt tgggatcaat acactaaaca atataccaaa
     6661 acgcatttaa atgattattt gctagataat attacttcat tgacaaataa attaaagaaa
     6721 aaatataagg tgaaatttgc aacgaagctt gttctaagat taatatttat tagatattta
     6781 atcgatagag gtgtcgatat agcttataaa aattttaatc atgatattaa agcttcacaa
     6841 aaagaactgc taaagattac taaaaacaaa gttgaattat atgaactgtt taaatactta
     6901 aaagaaaagt ttaatggtaa tttatttgag ttaaatgacg aaattaataa tacttgttta
     6961 acagatgaag tgtttatttt actatctgat tttttatcag gtaaaataag tattgacaat
     7021 ggacaaatgt ctttattttc aatgtatgac tttaatatca taccagttga actaattagt
     7081 aatatatacg aaattctttt aggaaaagaa attcgtaata aagataacgc attctatact
     7141 ccgaattact tagcagaata tattttagat aaaacaacac ttgaatttct gaaaaataat
     7201 aggcaataca aaattctaga tccttcttgt ggatcaggag tatttttagt aaatagtttt
     7261 agacgtatta ttgatatgaa tttgggtgat aaactttttt gtgaagatga taaactttta
     7321 aaagaacttt tattatctaa tatttatgga attgatatca atgaagatgc tatcgatgtt
     7381 actattttct cgttatatct aactatttta gattacaaag accctaagat attatcagaa
     7441 tttaaactgc ctaatttaaa agggtgtaat ttaatagcga gtgatttttt tgatgataat
     7501 aaattggaga gtttaaagaa aattaacttt gattttatca ttggaaatcc gccttgggga
     7561 agtgtctcag atggattaca tttagattat tgcaataaaa atggctataa tgatatgcaa
     7621 caaaacaaag aaatcagtag aagttttatt tttagagcaa aagattttag ctctagaaat
     7681 acagtatgtt cttttgtagt gcattctaaa atactatata ctcaaaaaaa gccatcaaaa
     7741 aaattccgtg aatttttgct taaaaaaagt caaatattta gtgtagttga aatgtcatct
     7801 gtaagaaaat tagtgtttaa aaatgcagac gctcctgcag cagttatatc atttaagtat
     7861 aacgaaagtg aagaaaattt aaataataga atcatctata catctttaaa accaaatgta
     7921 ttttttaaat tatttaacat tattgtaatt gaaaaatatg atgttaaata tatttcacaa
     7981 aaactattat tggagaatga ttgggcttgg aaaactattg tgtatggatt cagtaaagat
     8041 atagatataa ttatgaatat caaaaaaaaa tatcaaactg taaatgaaat tattttgaaa
     8101 aacagttttg ttcatggaac agggatacaa atcaatggta atgaaagcca tgatgcgaca
     8161 catttaaaag gaaagtggta tgttgattca aagaaaggaa tagattcatt ctatgttgat
     8221 ataaataaag ggaaagaact tactcaagat aaaatgagaa gacctaaggc taatcagcaa
     8281 gaaatattta ttcctccata tgtattatta aaaaagggag ttgacacgaa aacatataga
     8341 tatagagcag cattttcaaa tgatgcattt gtatattcag atgcagttac aggaatatgt
     8401 ggaaaaaaag ataatttaac tacactttat tgcttgactg gtatgcttaa ctcttctttt
     8461 tactcatact taaatataat gctaggtaca tcaattggaa tagaaagaga acaaggattt
     8521 tcaacggaaa tttttacgta tccagcaata gaaagtgatg aaattgctaa tctggtagta
     8581 aaaatacaag aagcgactaa attagaaaaa acaactatgt tcttgaatga aaaaaatagt
     8641 tgtaagtata tcaaacaatt agataagtta attttgaagt cttttaattt aaatgatgat
     8701 aaatttattg actatgcaat aaatattcaa attcctgagc ttacaaattc taagtcaact
     8761 aacatatata gaaaaataac taaggaagat ttaattaatt atagtgaatg tttcaaagaa
     8821 cagttttcat cgatatatcg tagggttggg aaaagtattt caatttcggt atatccaaaa
     8881 atattaaatc gttttacagt atttgaacta gaagtaacag atgaaaaaac agatagagag
     8941 attatggtta aagcagaagc tgataataat atggagttgt ttacgaaaat aattagatat
     9001 gattataatg atgtttttca tcaaattaga gatattattc attttggaga aaattcattt
     9061 tatatcataa agcctaattg ctataaatat tggcatcctg ctatcgcaga aatggatctt
     9121 gctgatgttt tggatcagat tttatctgat acagggggtg aagaataatg ggaggattta
     9181 aaaattcttc actaaacttt tatttaaagc aagggcttta tgaaataatt atagatggat
     9241 taatagactg ttgtttactt atgagcaaat catgtattga acaatctaga aaaatattaa
     9301 atcacgaaga aaagataaga acgcatttag ttgaaaatta tttagaaaat gatattgaaa
     9361 gaagaaaaac atctttatct aaacaacatc taagatttga agtagaagca caagaaaatt
     9421 atgacaacaa aaatgaatct tatgagggta gggttgatat aaaagttata tctaaagaca
     9481 ctttagcgga tagaagggct tattatatca ttgaatgtaa aaggatagat ggtaaggaaa
     9541 ctttaaataa aaaatatata accgaaggtg tacaacgatt tattactgag aaatattctt
     9601 cgcattatgg caaaaatata atgcttggat ttatagtaaa agatataaat atccagagca
     9661 atatacaaaa aattaattca ctgaatcaga acatattata tacatatata gatacagata
     9721 tgttgacagt tagaagtgat tctgaaaata attatgctgt ttgtgaaagt aaatataaag
     9781 taaaatctaa actactattg ttaaatcata ttttttataa cttttcgagt ataataagat
     9841 aatcttatga atataaaata ttactatgtt aaaattatag tttggttttg ataatattat
     9901 aaaaataagt ttaagaataa aatataaagg aaaaaagggg cgtagcacta ttttttagtg
     9961 tgacagcccc tttaatttta aaatcggtaa ggaggagtgt atttgaaaaa aattaagtca
    10021 tatacaggga ttttgaatgt ggagaaggtt atatatgcaa taaatgattt tcagcttcca
    10081 tttccagtta cttttacgca gatgacatgg tttgtattaa cactttttat aattatatta
    10141 tttggtaagt tacctccatt atttatgata gaaggagcat ttttaaagta tcttggcata
    10201 ccaattttta taacttagtt tatgtctcaa aagacttttg atggaaagaa accatatgga
    10261 tttttaaagt caataatact atattcatta agacctaaaa taacatacgc aggaaaaaac
    10321 attaaattta aaaataaaat tattgatgaa aatataacta tagtaaggag tgaattatat
    10381 gttcccgata aaatatatag agaacaatct tgtatttaat aaggaaggag aatgttttgc
    10441 atattatgaa ttaataccat ataattattc attcttataa gctgaacaaa aatttattgt
    10501 acatgataat tttaggcaat taatatcaca aagtagaaat ggtaaaattc atgcacttca
    10561 aatagccact gaaagttcta taagagttac acaagaaagg ttaaagaaaa atattacagg
    10621 aaaattaaaa gaaattgctt atgaaaaaat agacgagcaa acagaggcac ttgtttctat
    10681 gattggcgat aatcaaattg attatcgttt ttttattggg tttaagcttt tagttacaga
    10741 gaaagaaatg aatataaaga gtataaaaaa gtcaatatta gctaatctat cagattttat
    10801 atatgatgtt aataataaat tgatggatga ctttgtcagt ataaataatg atgaaattat
    10861 gagattttta aaaatggaaa agttattaga aaataaaatc tcaagaagat ttaaagttag
    10921 aaggttagat aaaaatgatt ttggatattt aattgaacat aattatggaa agaaaggcac
    10981 agtttttgaa gattatcaat accatttacc tacaaagaaa cttaaaagag aaacattagt
    11041 aaagaaatac aatcttataa agccaactag atgtcttata gaagagaagc aaagatactt
    11101 aaaaattata catgaggatt cagaaagttt tgtagcttat tttactattg aaattattat
    11161 tggagaaatg aattttccat ctagtgagat attctattat cagcaacaac agtttgcatt
    11221 tcctattgat actagtatga atgtggaaat tgtaacaaat aaaaaggctt taactacagt
    11281 tagaaataag aaaaaagaat taaaagattt agacaatcat gcttggcagt cagggaatga
    11341 aacagtaaca aatgtagtag aagcattaga ggctgtaaat gagcttgtaa ataatttaaa
    11401 tcaaacaaaa gaatcaatgt ataagttaag ctatgtaatt agggtatcag cagatagcat
    11461 tgatgaatta aaacgccgtt gtgatgaagt taaagatttt tatgacgatt taaatataaa
    11521 attagtacgc ctaatgggag atatgatagg atttcacaat gaatttatac cttcaagcaa
    11581 aaggtatata aatgattata ttcaatatgt aactagtgat tttctagcag gattagggtt
    11641 tggagcaact caatagcttg gagagaacga aggaatatat attggatata gtttagatac
    11701 aggaagaaat gtctatcttc aaccatctct tgcaagtcaa ggagtaaaag aatctgtaac
    11761 taatgcgttg gtagcagcat tctttggatt tttaggaggt gggaaatcat ttgctaataa
    11821 cttacttgtt tactattcag tattgtttgg agggcaagct gttttggtgg atccaaaagc
    11881 tgaacgtggc aaatggaaag aaaatttacc agatatatat catgaagtaa atattgttaa
    11941 tttaacaagt gatatagaaa ataagggaat gctagatcct tatgtaatta tgaaagaagt
    12001 aaaagatagt ggaagtctag ctattgatat attaactttt cttacaggaa taagtagtca
    12061 tgatggagag cagtttccta tacttagaaa atcaatcata aatgttactc aagacaaatg
    12121 cagaggactt ttgaaggtaa tagatgaatt aaggaaagat aattctcaaa tatcaaataa
    12181 tattgcagat catattgaaa gttttacaga ttatgatttt gcacaattac ttttctcaga
    12241 tggaaatatt aaaggctcaa ttaatctaga aaaacaaatg aatataatac aagttgcaga
    12301 tcttgtttta ccgaataaag atactttatt tgaagaatat acaacaatgg aaagtctttc
    12361 agtagctatg ttgatagtaa ttagtacttt tgcattagat tttatccata cagatagaag
    12421 tacttttaaa atagtagatt tagatgaagc ttggacattt cttaatgtag cacaaggaaa
    12481 aactttatct aataaattag ttagagcagg atgtgctatg aatgcaggag tgtattttgt
    12541 aactcaaaat gcaggtgatg tatcagatga aaatttaaaa aacaatattg gattaaaatt
    12601 tgcattcaga agcacagata tcaatgaaat taagagaata cttgaatttt ttggagttga
    12661 taaggaagat gagaataatc aaaaaaggtt aagagagtta gagaatggac agtgtttaat
    12721 acaagattta tatggacgag ttggggtaat acaaatacat ctagtttttc aagaactcct
    12781 tactgcattt gatacaagac cacctataag aaaagaggtg gattaatgaa atcgaaagta
    12841 tttagaaatt taagctggat aactatttta atagtagtac ttttactatt ttttatttat
    12901 ataaatttat agatttagag aaacaaatag atacattaga taagaaaaga aaaaaactca
    12961 tagatatgag attagaagat ataattgata aagaatcata tgaagaaaag tatgatgatt
    13021 taacagttga aataaagaaa cttagtaaag agaaaaaaac tcttaaagca tcattaataa
    13081 atgaaaagag tataaaagca agacttagag aatttagaaa gatacttgaa agaaatgaat
    13141 tgttagaaaa atttgataga tatgtatttg caagtatagt agataaggtt atagttggag
    13201 ttattaatga agatggtata gttgatcctt ataaaataac ttttgtttat aaaactagct
    13261 ttgatgataa acaagatgga cataaattta taaataagag tccgttagaa acgtattcaa
    13321 aagataacaa cgataataaa aagaagtgtt catatactaa taatgagaca ttgcgagact
    13381 gtagttaaac ttattagaga ataacaaggt ttcagacttt tatattgtgt cttaaaagta
    13441 gatttgccac ctatttgcca ccataattat ttttgggtgg caaatttatt ctacaatgat
    13501 ttcaaaaata tttaccgtct ggtttctcat gttgttagtt acatgtaaat atgtatccat
    13561 agttgttgaa gttttagcat gtccgagtat atgctggata tcttttatat tagtaccatt
    13621 ttctaataac atagtatcag ggtttttaaa ataaagtgtg tattcttcta aagtagtgat
    13681 aattaaaata tcaaatatca ctatacaaag gaaagtgcac gctttttatg tttaacaaaa
    13741 acgaaatatt aaaaaactta ttgaagaata tgatgtcaaa acaactacag atattcaaga
    13801 tatgctaaaa gatttattta caaatacaat tcatcaaata tttacattgt cgaattttag
    13861 cggtttaaac ataatatatt gactgatata ataaaatcat agaatatttt agggggaaat
    13921 agattatgtt taagaaaaag ttaatagctg ttgcaatgag tataactatg attagtgtag
    13981 ggagcttttc gtatgctcat tcaggaagaa ctgattcatc aggaggtcat cgtgataata
    14041 aaaataaatc tggattagga agttatcact atcattgtgg tggttatcca gctcatttac
    14101 ataataatgg tggttgtcct tatactggag gaggaagcag ttctggaaca acaacttcgg
    14161 ttaataatga agaaaagcaa aagaggtctg ttggagaaaa aggatataat caagggtatg
    14221 aagatgggta taaaggaaat tatagttcat caaattatag tggagattat agtgatacat
    14281 atgaatcaaa gtactcagaa ggatatgaaa aaggtaaggc taaattagaa gaggaagaaa
    14341 aggtggctaa agagactgga tataatttag ggcttacagg tgctaaatct aataatacat
    14401 atgaaaaaga agttttaaaa aatgcttatg ataccggata ttcaactgga tacaatgaat
    14461 ataagactaa aaagatagaa gaatataaag ctaaagggat agaggatagc aataaagata
    14521 aggaaaaaat gacatttgaa gaaaacatag attctgaatt tatagatgct tataataatg
    14581 cttatgacga aatacaagaa caattaaaaa atgattacac tactcaagga tttgagagtg
    14641 ctatcaaagg agaaagattt gacacatcaa ctataggtaa tgttaaatat gctaattggt
    14701 ttaaagaagg atatgataat ggtaaggtta agttacctaa agcaaaagaa agtgtatata
    14761 atcaaggtta caatgaagaa gattttagta taccagatga aatgaagtct attgaaacta
    14821 agttaaaagg tgtatatgat gaaggtttag aaaaaagaga agaagagaaa agtagaaatg
    14881 ctacttatgg ggttggagct ggaacagcag ctgtagttgc tggagtagta tataagaaga
    14941 aaaaacgtaa aaagatatag atgctagata taccaatgat gacaagtgta taacaaatat
    15001 aattttaagt ataaatattt aaaatataga agggaaggtt atatctatgg gatttaactt
    15061 taggaaaagc ataaatctag gaggaggact caaattaaat ttaggtaaga agagtgtagg
    15121 tataagtgct ggtgttaaag gtgctagagt gtcagttaat tcaaaaggaa gaaaatcagc
    15181 tacattgagt atacctggaa ctggaatatc atatacaaaa actagtacaa agaaacgtaa
    15241 acctaaataa atatttgata tactaagaaa tagactgtta caaatagtct attttttagt
    15301 atatagattt aaaaagtgac aaaattttga aatataaact aatttcataa aggaaaatat
    15361 tattttatgt agaattataa tatttaagta atattataaa atttatagga ggatttcatt
    15421 atgaaattaa gcaaaatact attggcaata gggatgtctg tttcattatt gattgctagt
    15481 tgtccaacat caaacgctct tagttctatt aattcaataa aaggtacaaa taagtatgaa
    15541 acagcaggtt taatagcaga taaacaaaat tatgatattg caatacttat taattctgaa
    15601 aatggattat ctgatggatt gtcagcaagt ggattgtcag gagctaaaaa tgccccaata
    15661 ttgttaacta agaaagatac aatcccttca gaaacattaa aaagattaga aaaggtaaaa
    15721 gaagtatatc taattggagg aaaaaattcg ataggagcat cagttcagaa aatattaagt
    15781 gataaaaaaa taagcataaa tagaataagt ggaactgata gaatagatac aagtcataaa
    15841 gttgcaaaag aaataggaag agtaagtacg gttaaaaaag taatattaac taatgctttt
    15901 aaaggcgaac cagatgctat gagtgttgct tctatagcag ttagagataa atctccaata
    15961 gtattaactg atggaaatac tacaagtttt gctacagatg gattagaaac ttatgcaatt
    16021 ggtggaaaag tcagtatgag tgataagtta ataaatgaaa ctaaagcaac aagaataggt
    16081 ggagtagata gatacgatac aaataaacaa ataataaata aattttataa tggctcaaaa
    16141 gaattttaca tagcaagtgg gactgattta gtttattctt tagttggttc tacaattgca
    16201 caaaaaacac ctatagtgtt agttggaaat ggaagtaata agagtatatt aaaaaatgca
    16261 agtaaaataa ctgctattgg aaatattgat gatgttgtta taaaacaatg tttaaatata
    16321 actaataatg ttggggattc aaatacaggt attgtaaaag atactaattc aaataatacg
    16381 aacaaaccaa gtaatgagcc tagctctaat aatcctggat taactgataa aagtacagta
    16441 tatgtaactc ctaatggaaa atcatatcat ctaacaaaaa attgttctac actaaaaaga
    16501 agtaaaacaa taaataaagt aacattaaaa caagctaagt cacaaggtaa aatagatcct
    16561 tgtaatgttt gtgtaaaata atgtattagc caaatatata ttaaaaaaga caattaatta
    16621 agattgtctt ttttattgaa gaaatataaa tttatattat gtattattaa aaaaaaatta
    16681 ttaattataa aaaataataa taaagacgat aagtattaaa attttatgtt tccaatattt
    16741 atgatataat taatatggca aggaataaat atttgaaaaa gctatgagtg gtgtttccat
    16801 ggaagtttct tcactttctt atgagaggag gtgaaaacta tggaaaatct aatgatgagt
    16861 attataactg gtgttatagc cagttacatt tacgataaat taaagagcca ctctgagcga
    16921 ccaactaaga gtggctggga acttaatata aagttccaga aaagtaaatc ttcaaaataa
    16981 tttgttgaag gaaacatcac tcaaagttaa gtaaaatatg tttccttgct tttattatat
    17041 cacaaattaa aaatattaac acaaaatttt gtgaatatat acttattatt gttctaaaaa
    17101 aatatgtaaa taattgattt gattgcatgg aattattgat gtaatattta agttaaatat
    17161 acacaatctc aattaaagaa gataagtact ataaattcat atcaaaaaaa tacataaact
    17221 tttaagttat aattttataa aaagtctttt agtatcatta tttcaagagg aattatcata
    17281 tgaaaaaata acttcattag aaaagtggat aaaaaactgg taagaagatg actaa
//
LOCUS       DAMJVK010000086        17185 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.087, whole genome shotgun sequence.
ACCESSION   DAMJVK010000086 DAMJVK010000000
VERSION     DAMJVK010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17185
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.087"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            171..800
                     /locus_tag="PC414_002861"
     CDS             171..800
                     /locus_tag="PC414_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465243.1"
                     /translation="MNKYLLINPVAERMYGEKFHLIKANLIKKGYIIAECEPQLEYVK
                     KQYKEYTKKTENTMLDCRCPESISLLKRNNLINDFEVPMIEPILVRTCRVLYDKYIKS
                     KDDMLVVTCPCTQLRDFASEKFKDKGNAMFYTWKEFAEQEGVKSLGKIDESPIPLGYF
                     KDTFEKVLEVSSEDEILSEIQKIRNGFEDKYDIVEMFYCKDGCNNGDGL"
     gene            801..1583
                     /locus_tag="PC414_002862"
     CDS             801..1583
                     /locus_tag="PC414_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2465244.1"
                     /translation="MVNKFNNKFLRLVKSVLIFFIILLLWKITNYLGIWSDYILPSPE
                     KVYSTFLNMISDGSIFINVYASMKRVLIGFAISTAIGVPLGIFFGIYSGVYEYFKSLI
                     NFLRNTPPLALIPMLILWFGIGEESKIIIIVLASFFPIFTSTLKGIKNCDSKLIEVGR
                     VFEFSKLQIIFKIIIPNAILDIAVGLKLALGYSFRAIIGAELVAASSGLGYLISDGKE
                     MSRTDVVIVGIIVIGLLGIITDYIFSIIVKKVSKGKMVEAYE"
     gene            1576..2454
                     /locus_tag="PC414_002863"
     CDS             1576..2454
                     /locus_tag="PC414_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2465245.1"
                     /translation="MNNLVCDSGYIIRNLYKSYKVDNEEHVVLSDISLNIDRNHITVI
                     LGESGCGKTTLLRAIASLEGINSGEIRFINDNKEYRPNVGFVFQESRLMPWLNVSENI
                     AFHNQRKNTILNKVLRKFKHNKRISSKIINDNFRSLETNDNLNKSNKYNRKSNIDVEK
                     YLKMMNLQKFKNSYPNELSGGMAQRVSIARALSFNPDMLLMDEPFSALDYFTRIDMQN
                     EVIRIHKSTNKGVIFVTHDIDEALKIGKKIVVFTDERKVKEFNIEDGYNRDLTSDYYI
                     KLKKDILMTMKSNKGI"
     gene            2487..3446
                     /locus_tag="PC414_002864"
     CDS             2487..3446
                     /locus_tag="PC414_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2465246.1"
                     /translation="MKLKKLLIGITILTLATASLAGCAKKNNGEKLSEINLTYVKSPL
                     NVPSIIQKQDDLFGKEFKKDNIKVNFYEITTGPEQTQALAAGEIDFLHALGGTSALIA
                     ASNGVELKILNTYSRSPKGFMILTNNNSIKSAADLVGKKVAGPKGTILHQVLISALDK
                     EGLSMDDVEFVNMGIPEASAALSDGSVDAALIAGPAALKAMKSGSKLVANGEGLVDGI
                     IVTAVSTDFAEKHPEIVERFMKVEKETLEYVNNNFDEAMEKVAKEVDLSLEETKELYA
                     WYDFSLDITDKDISSLEDTQDFLIKNKLQEKKVNIKELIYNAK"
     gene            3761..4453
                     /locus_tag="PC414_002865"
     CDS             3761..4453
                     /locus_tag="PC414_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2465247.1"
                     /translation="MSYKVYIVEDDISISTLLKDYIEKYGFEVIVEENFDDIMITFES
                     FKPHLVLLDVNLPKFDGFYWCRCIRQQSNLPIIFISARDGNVDQIRALESGADDYITK
                     PFYYDVVMAKIKSHIRRVYGEYSPKIDERIVELDGLKFYPERLELELNGENLIITKKE
                     GILIECLIKRYPKVVSRDYLLEKIWDDIEFVEENTLNVNVSRIRKRFEELGIDNAVQT
                     VRGLGYKLNKNW"
     gene            4463..5467
                     /locus_tag="PC414_002866"
     CDS             4463..5467
                     /locus_tag="PC414_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2465248.1"
                     /translation="MKLFLKDYKGYIFIYYIGIVMTIIYCNLMKFISFGESLYILLFS
                     SFILLCFLIYKYYKNREVYNLFEKGVHSLEESFLYLGSSFLGRNISVILKQQHKLYQS
                     MIQEHKKIHSEHLTFINQWIHQMKTPISVISLQLQDYEGEEVTENIRKEIDRLNKGLD
                     MAMHFARADNFQKDFIVEKAKLNEIVSSIVNEEKRLFIKSGMIPKIQVDKFTYVYTDV
                     KWMKFIIGQLITNGVKYSKDYSNYLTIKSEENNKYVILSIIDEGIGIVSKDLKRVFDP
                     FFTGENGRKFGESTGMGLYLVKKVCDDLGHKVSIKSEVGKGTEVSIYFNKDVNSLIKV
                     "
     gene            5735..6586
                     /locus_tag="PC414_002867"
     CDS             5735..6586
                     /locus_tag="PC414_002867"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDF2465249.1"
                     /translation="MSLDVIFILKSVIIAIVEGLTEFIPVSSTGHMILVGNLIDFKGQ
                     FAEMFEVVIQLGAILAVVVLYWKKIKDSVIEFFKFIFTGGKEGKIGFRFGMNVIIGCI
                     PFAIIGVLFYDNIKSLFNLQSVIIGFIVGGILLLVVETLFRKKNHSTDNIDKITPIQA
                     LKVGTLQVLSAWPGMSRSASTIMGGWIAGLNSPTAAEFSFFLAVPAMVASSGKDLFEF
                     DYSIMTPTLWIALVVGFIVAFIVSIIVMEKFVNFLKKKPMRVFAVYRIIMGVVLAVLA
                     FTNIISV"
     gene            6690..7436
                     /locus_tag="PC414_002868"
     CDS             6690..7436
                     /locus_tag="PC414_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001637630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2465250.1"
                     /translation="MKILTVNNLSKVYGKKIIFNALNDINFSIEDGEFVGIMGPSGSG
                     KTTLLNMISTIDKPTTGTMELKGKNPLLLRGEELALLRRRELGFVFQDFNLLDTLTIG
                     ENIVLPLTLDKVSVKEQDERLNEVSTILGIKDLLGKRTFEVSGGQAQRTAIARALINN
                     PSILLADEPTGNLDSKSSKVVMELFQKINKENKVTTMMVTHDPLAASYCSRILFIKDG
                     SIYNEIYKGSSREQFYQEIMDVLTLLGGDN"
     gene            7436..9352
                     /locus_tag="PC414_002869"
     CDS             7436..9352
                     /locus_tag="PC414_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2465251.1"
                     /translation="MNIKQFAINNISRNIKAYLGYLASIVISSSLLFSFIMFTSHPDL
                     DVSQFPDYLKEGLKMSKIIAYLFLFFFVFYSVSVFLKSRYKEFGILYITGISKRQVMK
                     LIFIENMIINIVSSVIGIIIGLIFAKIFLVIMSTFLELSPLKFYIPFNAILSTLIYFM
                     ILAVLTSIFTSFIVKENQVLKLLKGTKTPKPEPKTSLMLAILSICLFIIAYYNAITST
                     DQYTTYLRLVPVTSLTIVATYLFFSQFSVFFMKKLKLNKRFYRKNTNMLWISNLIYKV
                     KDNARIFFLITITSAVAFTAIGTVYSFWKDVERQINLIYPNTIYYSTMTLHNDPKKPD
                     SKEKDKERMMFIENNLKKENVDYTRTNGEFKTVFPKKSDFNVRIMKESKYLEITETIG
                     VKSISFEDNECISLLSSSLPGNNNVKENIIVGNRGLKVAKQVEECVMPAYYKNMYVVK
                     DNFYDSIESKFIVDRFTAFEVEDSSKTIEICREFENKFNNESGMQPYLFFSKELMIGT
                     GKLMYSTFIFLAIFIGLIFFVTTSSFLYNKFYMDCQVDKKKYEQLNKLGMTYNEIKKA
                     STIEIGIVFLFPYVVAVIHSIFALTALKNSFDIEVASSAVLVMGSLFLVQIVYFFIVR
                     NNYLKEIRLNLVNF"
     gene            9632..9865
                     /locus_tag="PC414_002870"
     CDS             9632..9865
                     /locus_tag="PC414_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HDF2465252.1"
                     /translation="MMILIQSRGKMKCKELSEELEVSERQIKSYKMYLEQAGIFINST
                     PGIYGGYEIDKCNSISLIKLLDSEVSIFISTKW"
     gene            10061..10489
                     /locus_tag="PC414_002871"
     CDS             10061..10489
                     /locus_tag="PC414_002871"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDF2465253.1"
                     /translation="MIKKLNSKDINKIMEIWEKSTIKAHDFISKEYWQNNYNTVKNEY
                     IPISDTFVYDDGDEIKGFISIIDKNFIGALFIKPKYQNLGIGGKLLDYATKKYKSLSL
                     AVYKDNKKAVVFYNKKGFNIVKEQVNEDSGFKEYIMEYSK"
     gene            complement(10943..11713)
                     /locus_tag="PC414_002872"
     CDS             complement(10943..11713)
                     /locus_tag="PC414_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465254.1"
                     /translation="MKVNENNIDEELDELSRKVYLESPDFWISISDKIIDKVFDEMLL
                     FFAVKYNYFNIVCFAIENNYLDLNMPSKNKKYTNIMEHLLDTSKQNEDKKIYNYLLSL
                     NKGNNLDERPILDELNCNISSDTFEKKDIYLPKYLCPSCNSNIFDTGYKVSNDITYKF
                     SSKEAEPIEMCSEVSSIRCCNCDELLEDITLDKLYNLCKIQNCNNCGSNLTKVGIIAK
                     KKMNFDENTEKFKDVSTFYCCGNCDSEISLSQREHFKI"
     gene            12037..12582
                     /locus_tag="PC414_002873"
     CDS             12037..12582
                     /locus_tag="PC414_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HDF2465255.1"
                     /translation="MKNTKVNIQDLTKMSICVALLCISSYIYIPLPFTPAGVTAQTIM
                     INLIALILTPRQAFSTVGVYIMIGLIGIPVFGGGTSGIGKLLSPTGGFYFGFLFAALI
                     ISLLKGRNNDIKRYILITIFVGMPIIYFMGTVFMCYFNQMTVEAALFAAVVPFLIGDT
                     IKSVIASFLGTKLNNVLRRNL"
     gene            12614..13750
                     /locus_tag="PC414_002874"
     CDS             12614..13750
                     /locus_tag="PC414_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HDF2465256.1"
                     /translation="MKEVFILGGLRSHIGLKNGIFQFVQPELLGASVLKDLIKKYEID
                     KIDEIICGNAVGTGGNIARLMTLTAGVSNEVPAFTVDMQCASAMMSIDIAFSKVKSGQ
                     CDLIIAGGFESSSLQPMRTYHKNDKRYNTNNPNYTVAQFSPDDNSQNSMLEGAERVAE
                     LYQIEKADLDFWVKESHKRAKEAREEKILEDIISPINNSTYDEGIRDKMSQRLLDRMP
                     SILGKETITNAANSCLINDGASFIIICSKKYLEHVKKKPKAKIINTCTIGTDATLSPT
                     SAIQAMDKLLEIESLNYLDVSAVEFNEAFAVIDVLFQRKYPELIDRYNIFGGALAYGH
                     PYGASGAIIALHLLKALEKTKGRYGICSIAAAGGLGSALLLERV"
     gene            13754..15145
                     /locus_tag="PC414_002875"
     CDS             13754..15145
                     /locus_tag="PC414_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HDF2465257.1"
                     /translation="MNYYELLRIKKERHKNKNFLIIDGEKYTYENILSYSEELGKQIS
                     LGENIPILIYSKNIMFQLISFFAINYSKNIPIICHYNLSKKVLDDILLKNNISIIISD
                     ESMDVIDLYDDNNNEVAKKVNFSIFNPKFNIYMYQYKKLINYLDKSICMGALSSGSTS
                     VPKVLYRTYESWAGFFPIQNGIFNISGESILFVNGTLSFTGNLNSIMSVLYEGGSIVI
                     SSGLNCKSWIRTIKQYNVTNIYLIPTKLQLLVKHLKEPVLKVVSIFTGSQLLFEDTAR
                     NLKKYMPKSEIILYYGASELNYITYLCYDELIEKPLSVGRPFPGIDIYIREGKIFVNT
                     EYAVYNATKPYSVNDIGYYDNDGYLIFEGRSDDIINIGGFKVSSTKIENEVKKIPQIE
                     DAIVLPYSDTIRGNQIVLFVITIDKITKKDLLIKMKDNLMKNEIPKKIIFLNSFPYTS
                     SEKIDRLALLKML"
     gene            15221..16096
                     /gene="dpsA"
                     /locus_tag="PC414_002876"
     CDS             15221..16096
                     /gene="dpsA"
                     /locus_tag="PC414_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit DpsA"
                     /protein_id="HDF2465258.1"
                     /translation="MSNQYDITVIGGDLRQVYMVKKLINKGYSVVTYGISNEIINGIV
                     GKSTSLAEALSKSSIILCPIPFTKNQVNIENTDDCLDATIDNLLINLSPNQILFGGNI
                     PTKVCKFCEQHSIQIYDFMKMNDVAILNAVATAEGAIAEAIKRSIINIHQSNCLVLGY
                     GRCAQILAKKLYALDANVCVGARNNDARSTANAFGYSSISLSQLDEKLLQFDYIFNTI
                     PECILTKERLEKVSQEVTIIDIASSPGGVDYSVVKEAGINATLCLGLPGKYSPKTSGE
                     ILVNAIENIINERRD"
     gene            16099..16689
                     /locus_tag="PC414_002877"
     CDS             16099..16689
                     /locus_tag="PC414_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit B"
                     /protein_id="HDF2465259.1"
                     /translation="MRLEGLTIGVGFTGSFCTYDKIFIELENLVKEGANVHTIFSDVS
                     QNIDCRFGNSEEFMKKAYELTGNKPIVTIEEAEPFGPKGIADIIIIAPCTGNTAAKLA
                     NGITDSPVLMAAKGHLRNDKPLVISISTNDALSFNFKNIGILLNSKNIYFVPFGQDNC
                     KAKPNSMIAHTELIIPTIELALENKQLQPVIKSPHQ"
     gene            complement(16896..17096)
                     /locus_tag="PC414_002878"
     CDS             complement(16896..17096)
                     /locus_tag="PC414_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465260.1"
                     /translation="MEKLKTISVFSLIISVILTIGGIGIVTYYVDNLFIRGLSVFVLI
                     MSSSFVSTTVRLIFEESKRYKF"
BASE COUNT         6710 a         1760 c         2828 g         5887 t
ORIGIN      
        1 attattaatt aaaataaatt acatatacta tttaatattt catttaaaat attaaaaaat
       61 gtagataaaa attgttttat taagaaaaaa ttaatatatt gacaaaattt aagtgctcat
      121 ttaaactatt aattgataat aatattcaat attaaataat aggaggcgtt atgaataaat
      181 atcttctaat aaatccagtg gcagaaagaa tgtatggaga aaagtttcac cttattaaag
      241 caaatttgat aaaaaagggg tacataatag cagaatgtga accacagtta gaatatgtaa
      301 aaaagcagta caaagagtat acaaaaaaga ctgaaaatac aatgttggat tgtagatgtc
      361 cagaaagtat aagtctttta aaaagaaata atttgataaa tgattttgaa gtacctatga
      421 tagaaccaat attagtaagg acttgtaggg ttttatacga taaatatata aaaagtaaag
      481 atgatatgtt ggtagtaacc tgtccatgta cacaattaag agactttgca agtgaaaagt
      541 ttaaggataa gggtaatgct atgttttata catggaaaga gtttgctgaa caagaaggtg
      601 taaaatcact tggaaaaata gatgaatcac caattccatt aggatatttc aaagatactt
      661 ttgaaaaagt tcttgaagta tcaagtgaag acgaaatatt aagtgaaata caaaaaataa
      721 gaaatggctt tgaagataag tatgacatag tagaaatgtt ttattgtaaa gatggttgta
      781 ataatggaga tggtttgtaa atggtcaata aatttaataa taagttttta aggctagtaa
      841 agagtgtttt gatatttttt ataattcttt tattatggaa aattacaaat tatcttggta
      901 tttggagtga ttatatatta ccatcacctg aaaaggtata tagtacattt ttaaatatga
      961 taagtgatgg ttcaatattt attaatgtat atgcaagtat gaaaagagtt ttgataggtt
     1021 ttgcaataag tactgctata ggtgtgcctt tagggatatt ctttggaatt tatagtggag
     1081 tatacgaata ctttaaatct ctaataaatt ttttaaggaa tacacctccc cttgctttaa
     1141 taccaatgct tatattatgg tttggtatag gagaggaatc aaaaattata attatagttt
     1201 tagcatcctt tttcccaatc tttacaagca cattgaaagg gattaaaaat tgtgactcaa
     1261 aactgattga agtgggtaga gtatttgagt tttcaaagct tcagataata tttaaaataa
     1321 taataccaaa tgccattctt gatattgctg ttgggttaaa gttagctctt ggatatagct
     1381 ttagagccat tattggggca gaacttgtag cagcttcatc tggtcttgga tacttgatat
     1441 ctgatggaaa agaaatgtct agaacagatg ttgttattgt aggtataatc gtaattggac
     1501 ttttaggaat aattacagat tacatatttt cgattattgt gaagaaggta agtaagggaa
     1561 agatggtgga agcatatgaa taatttagta tgtgactctg gatatataat tagaaattta
     1621 tataaaagtt ataaagttga caatgaagaa catgtagttc ttagtgatat ctctttaaac
     1681 atagatagaa accatataac agtaatttta ggtgaaagtg gttgtggaaa gactaccttg
     1741 cttagagcaa ttgcatcact tgaaggtata aatagtggtg aaattaggtt tattaatgat
     1801 aataaagagt atagacctaa tgttggtttt gtttttcagg agagcagatt aatgccttgg
     1861 ttaaacgtaa gtgaaaatat agcttttcat aatcaaagaa aaaatactat tttaaacaag
     1921 gtgcttagaa aatttaaaca caataaaaga attagcagta aaattataaa tgataatttt
     1981 agaagcttag aaacaaatga taatttgaac aaatcaaata aatataatag gaaaagtaat
     2041 attgatgtag aaaaatatct taagatgatg aacttgcaga aatttaaaaa ctcttatcca
     2101 aatgaattgt caggaggtat ggcgcagaga gtaagcatag ctagagctct ttctttcaat
     2161 ccagatatgt tgcttatgga cgagcctttt tcggcattag attattttac aaggattgat
     2221 atgcaaaatg aagttataag aatacataag tcaactaata aaggcgtgat atttgtaact
     2281 catgatattg atgaagcgtt aaagataggc aagaaaatag ttgttttcac agacgaaaga
     2341 aaagtaaaag aatttaatat agaagatggt tataatagag atttaacttc agattactat
     2401 attaaattaa aaaaagatat tttaatgaca atgaaatcaa ataaaggcat ttaaagaaat
     2461 taataatatt ttaaggagta aagtaaatga aattaaaaaa gctattgata ggtataacaa
     2521 tattaacact tgcaacagca tctttagctg gatgtgctaa aaaaaataat ggagaaaaac
     2581 tttcagaaat aaatttaact tatgtaaaat caccattaaa tgttccttct ataattcaaa
     2641 aacaggatga tttatttgga aaggaattta aaaaggataa tataaaagtt aatttctacg
     2701 aaattactac aggtccagag caaacacaag ctttagctgc tggagaaata gacttcttac
     2761 atgcactagg tggtacatca gctttaatag ctgcatctaa tggagtagag ttaaagatat
     2821 taaacactta tagcaggtca ccaaaaggat ttatgatttt gactaataat aattcaataa
     2881 aatcagcagc agatttagta ggtaagaagg tggcaggccc aaaagggact atattacatc
     2941 aggtattgat ttcagcactt gataaagaag gattaagtat ggatgatgtg gaatttgtta
     3001 atatgggtat tccagaggca tcagcagctc tttctgatgg cagcgtagat gctgcattga
     3061 ttgctggtcc agcagctctt aaagctatga agtcaggtag taaacttgta gcaaatggtg
     3121 aaggtttagt agatggtata atagtaactg ctgttagcac agactttgca gagaaacatc
     3181 cagaaatagt tgaaagattt atgaaggttg aaaaggaaac tttagaatat gtaaataata
     3241 attttgatga agctatggaa aaagtagcta aagaagttga tttaagtctt gaagaaacta
     3301 aagaattata tgcttggtat gattttagtt tagatataac agataaagac atatcttcgc
     3361 ttgaagatac acaagacttc ttaataaaaa ataagttaca agagaaaaaa gtgaacataa
     3421 aagaattaat ctataatgct aagtagaaac tatgataaaa taatactaag taagaaatat
     3481 agtaaaagtt taagttacct tattaaggta actttttcta ttttgaatta gtaatttcaa
     3541 tttattttag aatgattagt tgataataat gattaattaa tcataaaaag atactttttt
     3601 atagagtttt attaagatgt ctaataatat ggttatactt atgaagtcag taattgtgaa
     3661 gttagtaata tgattaaatt tatatattgt aaaaagatta taatatatta aaattggact
     3721 gttaatatga tgagtaaata ggtatattag gaggaattat atgagttata aagtatatat
     3781 tgtggaagat gatatatcaa taagtacatt acttaaagat tatattgaga aatatggatt
     3841 tgaagtgatt gttgaagaaa actttgatga tataatgatt acatttgaaa gttttaagcc
     3901 acacttagtt ctattagatg taaatttacc taaatttgat ggtttctact ggtgtagatg
     3961 tattagacaa cagtcgaatt tacctataat ttttatatca gctagagatg gtaatgtaga
     4021 ccaaattaga gctttagaaa gtggagcaga tgattatata acaaaacctt tttactatga
     4081 tgtagtaatg gcaaaaataa agagtcatat aagaagggta tatggtgaat attcacctaa
     4141 aatagacgaa agaatagtgg agctagatgg attaaagttt tatccagaga ggttagaact
     4201 agaattaaat ggtgagaatc taattataac taaaaaagaa ggtatactga ttgaatgtct
     4261 tatcaagaga tacccaaaag tagtaagtag agattatctt ttagaaaaga tatgggatga
     4321 tattgagttt gtagaggaaa atactttaaa tgtaaatgtt agcaggataa ggaaaagatt
     4381 tgaggaatta ggtatagata atgctgttca gactgtccga gggttaggat ataaattaaa
     4441 taagaattgg taaggagaaa gtatgaagtt atttttaaaa gattataaag gttatatttt
     4501 tatatattat ataggcatag ttatgacaat tatttactgt aatcttatga agtttattag
     4561 ttttggagag agtttatata ttcttttatt tagttctttt atattgttat gttttttaat
     4621 atataagtat tataaaaata gagaagtata taatctgttt gaaaaaggag tacattcttt
     4681 ggaggagtca tttttgtatt taggaagctc attcttgggg agaaatatat cagttatttt
     4741 aaaacaacaa cataaattgt atcaatcaat gatacaagag cataaaaaga tacatagtga
     4801 acatttaact ttcatcaacc aatggattca tcagatgaaa acaccaatat cagttataag
     4861 ccttcagctt caagattatg agggagaaga agtaactgaa aatataagaa aagaaataga
     4921 tagattaaat aaagggttag atatggctat gcattttgca agggcagata attttcagaa
     4981 agactttata gttgaaaaag ccaaattaaa tgaaattgta tctagtattg taaatgaaga
     5041 aaaaaggtta tttataaaaa gtggaatgat tcctaagatt caagtagaca aatttactta
     5101 tgtttataca gatgtaaaat ggatgaaatt tataataggt caattaatta caaatggagt
     5161 gaaatattct aaagattata gtaactacct aactataaaa tctgaagaaa ataataaata
     5221 tgtaatttta agtataatag atgaaggaat tggaattgtt tctaaagatt taaaaagggt
     5281 atttgaccca ttttttacag gagaaaatgg tcgtaagttt ggagaatcta caggtatggg
     5341 tctttatcta gtaaaaaagg tatgcgatga tttaggtcat aaagtaagta taaaatcaga
     5401 agtaggaaaa ggtacagaag tttcaatcta ctttaataaa gatgtaaata gtttaataaa
     5461 ggtgtaaata gtttgataaa gatgtaaatt atctgttaca atagggtttt tagttaattg
     5521 tactttttta ttatacaatt tattaaatga aatattcata gttaaataat caaaatttaa
     5581 gagcgtttat ttgcaaacct tacatagatg taaggtttgt gtaatgtttg cagatagtta
     5641 aattttctaa tctaaattac aattaatata agaaataaca taagagaaat aacataaaaa
     5701 aagaaatttt gtaaaaaagg aaggatgttt attgatgagt ttagatgtta tttttatatt
     5761 gaaatcagtc ataatagcta tagtagaggg gcttacagaa tttattccag tttcatctac
     5821 aggtcatatg attttggttg gtaatttaat agattttaag gggcaatttg ctgaaatgtt
     5881 tgaggttgta atacaacttg gggctatatt agcagtagta gtactttatt ggaaaaagat
     5941 aaaagatagc gttatagaat tttttaaatt tatatttact ggtggaaaag aaggaaagat
     6001 tggttttaga tttggaatga atgtaattat aggttgtata ccatttgcga ttataggagt
     6061 tctattttac gataatataa aatcattgtt taatttacaa tctgtaatta taggatttat
     6121 agtgggtggg attttattat tagtagtaga aactttgttt agaaaaaaga atcattccac
     6181 agataatata gataaaatta ctccaataca agctttaaag gttgggacat tacaagtttt
     6241 gtctgcatgg ccaggtatgt caaggagtgc atctactatt atgggtggat ggatagcagg
     6301 acttaattct cctacagcag ctgagttttc tttcttttta gcagtaccag ctatggtggc
     6361 ttcttcagga aaagatttat ttgaatttga ttattcaata atgacaccaa cattgtggat
     6421 tgctttggta gttgggttta tagtagcttt tattgtatca ataatagtta tggaaaaatt
     6481 cgtgaatttt ttaaagaaaa aaccaatgag agtgtttgct gtatacagaa taataatggg
     6541 tgtagtactt gcagtattag cttttactaa cataattagt gtatagaaaa aatagattta
     6601 tcatctataa aaattaaact aagataaatt ataattaaca attatatata tgttatttca
     6661 aaaattaaat taggaaggat agttaaataa tgaaaatttt aactgtaaat aatttaagta
     6721 aagtatatgg taaaaaaata attttcaacg cattaaatga cataaatttt agtatagaag
     6781 atggagaatt tgtaggaatt atgggtccat ctggtagtgg aaaaactact cttttaaata
     6841 tgatttctac tattgacaag ccaactacag gaactatgga attaaagggc aagaatcctt
     6901 tattgttaag aggtgaggaa cttgcattac ttagaagaag agagttaggt tttgtatttc
     6961 aggattttaa tttattggat acattaacta taggagaaaa tatagtattg ccattgacat
     7021 tagataaggt cagtgtaaaa gagcaagatg agagattgaa tgaagtatct acaatattag
     7081 gtataaaaga tttattagga aaaagaactt ttgaagtatc aggtggtcaa gctcagagga
     7141 ctgctattgc tagagcattg attaacaatc catcaatact tttagcagat gaaccaacag
     7201 gtaatttaga ttcaaagtca tcaaaggttg taatggagct atttcaaaag ataaataaag
     7261 aaaataaagt tactactatg atggtaactc atgacccatt agcagcgagc tactgtagta
     7321 gaatcttatt cataaaagat gggtctatat ataatgaaat atataagggt agtagtagag
     7381 agcagtttta tcaagagata atggatgtac ttacattatt gggaggcgat aactaatgaa
     7441 tataaaacag tttgctatta acaatatttc aagaaatatt aaagcgtatt taggatactt
     7501 agcaagtatt gttatatcat cttcactgct attttctttt attatgttta caagtcatcc
     7561 agatttagat gtatctcaat ttcctgatta tttaaaagaa ggtctcaaga tgagtaagat
     7621 aattgcatat ctattcttat tcttctttgt gttttattca gttagtgtgt ttcttaaaag
     7681 tagatataaa gaatttggaa tactttatat tacaggtata tcaaagcgcc aagtaatgaa
     7741 attaatcttt atagaaaata tgataataaa tatagtatct tctgtaattg gaataataat
     7801 aggcttaatc tttgcaaaga tatttttagt gattatgtct acttttttag aattatcacc
     7861 attaaaattc tatataccat ttaatgcgat attgtctact ttaatctatt ttatgatttt
     7921 agctgtatta acatcaatat ttacttcttt tatagttaaa gaaaatcagg tgttgaagtt
     7981 acttaaaggt acaaaaactc caaaaccaga gcctaaaaca tcactaatgc ttgctatatt
     8041 aagtatttgt ttgtttataa tagcgtatta taatgctatt acctcaaccg accaatatac
     8101 aacatatctg cgcttagttc cagttacatc ccttactata gtagcaactt acttgttctt
     8161 ttctcaattt agtgtatttt ttatgaagaa gctaaaacta aacaagagat tttatagaaa
     8221 aaatacaaat atgctttgga tttcgaactt gatttataag gtaaaggata atgctagaat
     8281 atttttcttg ataactataa cttctgcagt agcatttact gctataggta cagtttattc
     8341 cttttggaaa gatgtagagc gtcaaattaa tttaatatat cctaacacaa tatattattc
     8401 aacaatgaca ttgcataatg accctaaaaa gcctgatagc aaagaaaaag acaaggaaag
     8461 aatgatgttt atagaaaata atctaaaaaa agagaatgta gactatacaa ggactaatgg
     8521 tgaatttaaa actgtatttc ccaagaaaag tgattttaat gtcagaatta tgaaagaatc
     8581 taagtactta gaaattacag aaactatagg ggttaagtct ataagttttg aagataatga
     8641 atgtatatca ttattatcat caagtttacc aggaaataat aacgtcaaag aaaatattat
     8701 tgtagggaat aggggtttaa aggtggcaaa acaagtggaa gaatgtgtta tgccagcata
     8761 ttacaaaaat atgtatgtgg taaaggataa tttttatgat agtatagaat caaaatttat
     8821 agtggataga tttactgcat ttgaagtaga ggattcaagt aaaactatag agatatgcag
     8881 agaatttgaa aataaattta ataatgagtc agggatgcaa ccatatttgt ttttctctaa
     8941 agaattaatg ataggaactg ggaaattaat gtattctaca tttatatttt tagcaatatt
     9001 tataggttta attttctttg ttactacaag tagtttttta tataataaat tttatatgga
     9061 ttgccaagtt gataagaaaa agtatgaaca gttaaataaa ttgggtatga catataatga
     9121 gattaaaaaa gcttcaacaa ttgagattgg aatagtattt ttgtttcctt atgtagtggc
     9181 tgttatacat tcaatatttg cacttactgc acttaaaaat tcttttgata tagaagtagc
     9241 atcatcagct gttttggtaa tgggaagctt gttcttggtt caaatagttt atttttttat
     9301 tgtaagaaat aattatttaa aagagataag gttaaattta gtcaattttt agttactatt
     9361 attaaatgat aataaaaagg gtttaaaatt aattaatatt actggaatta tagatgttta
     9421 ttttttcagt aatatttttt attgtattaa ctattaaaat tttgtacaaa tataaaatat
     9481 aatatggtaa atgtcaaaat tataaaaaaa ttctacttat ataaaatata aagaggttat
     9541 atgtacatat gtgtagaaat aaataataaa aatatcaatt aatgttatta ggggggggtt
     9601 actcattagt aaattagcta atacacttag aatgatgata ttaattcaaa gcagaggcaa
     9661 aatgaaatgc aaagaattat cagaagagtt ggaagtaagt gaaaggcaaa taaaaagtta
     9721 caagatgtat ttagagcaag ctggtatatt tataaattca acacctggga tttatggtgg
     9781 ttatgaaata gataaatgta atagcatatc attaattaaa ttattagata gtgaagtttc
     9841 aatttttata tcaactaagt ggtagataag tagaatatta gcaatatcaa cgagtattta
     9901 gaaacttatt ttatattaat aaagcagtat ataaaattgt agaatcaaca atagcatata
     9961 ctaaaacatc caccttatat taactaaatc atatctaaat aaaatacact tacctacaaa
    10021 cattataaaa tcaatacaaa aatgaggtga aacaaaattt atgataaaga aattaaacag
    10081 taaagacata aataaaatca tggaaatatg ggaaaaaagt acaatcaaag cacatgactt
    10141 tataagtaaa gaatactggc aaaataacta taatactgtt aaaaacgaat atatacctat
    10201 atcagataca tttgtatatg atgatggaga tgaaataaaa ggatttataa gcataataga
    10261 taaaaacttt ataggagctt tatttataaa gcccaaatac caaaatctag gtatcggagg
    10321 taaactttta gattatgcaa ctaaaaaata taaaagtcta agcttagcag tatataaaga
    10381 taataaaaaa gcagttgtgt tttataataa aaaaggtttt aatatagtaa aagaacaagt
    10441 aaatgaagat tcaggattta aagagtacat aatggaatat agtaaataat atgattacat
    10501 tctattaaaa ggataattaa tttattttct agttgatttt ttaagagtaa tttttaaaat
    10561 agactatatg aagataaaga tatttattat agatgtaagt taatcagaat tgatatagct
    10621 acaaaagagg aaaataaaat tgtatggtgt atttcagatt taacaaattt actatttaaa
    10681 gataataagt ttgatataat attaaagata ctttcaccta taaactatag agtattgaaa
    10741 gaattttaaa taattatgga atgctaataa aggtttgtta aaatttaata attattagat
    10801 tatagaaaat tgccatgaaa ttagttcatc aagaactagt ttcatagcaa ttttaattct
    10861 tataagtgta tttattttta cagatttaaa aaacttatac agtatctaat aatgtaatct
    10921 tattacttta gcctataaat atttaaatct taaaatgttc tctttgagat aagcttattt
    10981 cagagtcaca atttccacaa cagtaaaatg ttgagacatc tttaaatttt tcagtattct
    11041 catcaaagtt catttttttc tttgcaataa ttcctacctt agttaaatta gaaccacaat
    11101 tattacagtt ttgaattttg cataggttat atagtttatc aagtgtaata tcttctaata
    11161 attcatcaca attacaacat ctgatagaag aaacttcact acacatttca ataggttcag
    11221 cttctttaga agaaaattta tatgttatgt catttgatac tttataacct gtatcaaata
    11281 tattagaatt acaacttgga cataagtact ttggaagata tatatctttt ttttcaaaag
    11341 tatcactact aatattacag tttaattcat ctaaaattgg tctttcatct aggttattac
    11401 ctttatttaa gcttagaaga taattataaa tttttttatc ttcattctgt tttgatgtat
    11461 ctaacaaatg ctccattata ttagtgtatt ttttgttttt tgaaggcatg tttaaatcca
    11521 gataattgtt ttcaatagca aagcatacta tattgaaata gttatattta actgcaaaaa
    11581 ataataacat ttcatcaaat actttatcta ttattttgtc agaaatactt atccaaaaat
    11641 ctggtgactc taagtaaact ttcctactaa gttcatctaa ctcttcatct atattatttt
    11701 cattaacttt catatcatca cttcctttta aatatcttgt gtataaacta tttgttacta
    11761 ttttttatat aaaataaaat cttatataaa aatatatcat attataattt acaagtatag
    11821 tctaaataga gctgttttta agcaaagttt tataaatgac tattaaaata taaagtaaca
    11881 gtataaagtt tacttataaa ttaaatgtaa acctaaataa ttataatagt tgacaaaaaa
    11941 acgtctttat tttatactaa aacaaagctt taatagaagc tttgtatatg tacatataaa
    12001 ctaaatgaaa taagacatat gaagggaatt ataaaaatga aaaatactaa agtaaatatt
    12061 caagatttaa ctaaaatgtc aatatgtgta gcattattgt gtatatcatc ttacatatat
    12121 ataccattgc catttactcc agcaggagta acagcacaaa caataatgat aaatttaata
    12181 gctttgattt taacaccaag acaagcattt agtacagttg gtgtatatat aatgataggt
    12241 ttaattggga ttccagtatt tggtggaggt acatctggta ttggaaagtt attaagccca
    12301 acaggtggtt tttattttgg gtttttgttt gcagcactaa ttattagttt attaaaagga
    12361 cgaaataatg atattaagag atatatatta ataacaattt ttgttggaat gcctattata
    12421 tattttatgg gaacagtatt tatgtgctat tttaatcaaa tgacagtaga agcagcactt
    12481 tttgcagcag tagtaccatt tttaattgga gatacaataa agtcagttat tgcatctttt
    12541 ttaggcacta aactgaacaa tgtactcaga agaaatctat gataaatcta taatataagg
    12601 aagggtgtaa tgtatgaagg aagtttttat attaggagga ttgagaagtc atataggtct
    12661 aaaaaatgga atttttcaat ttgttcaacc tgaattacta ggagcaagtg ttttaaaaga
    12721 tttaattaaa aagtatgaaa ttgacaaaat tgatgagatt atatgtggaa atgcagttgg
    12781 gacaggaggg aatatagcta gattaatgac tttaacagca ggagtgtcaa atgaagtacc
    12841 tgcttttact gtggatatgc agtgtgcttc tgctatgatg agtatagaca tagctttttc
    12901 aaaggttaaa tctggtcagt gtgatttaat tattgcaggt ggttttgaaa gtagttctct
    12961 gcaaccaatg cgcacatatc ataagaatga taagcgatat aatacaaata atccaaatta
    13021 tacagttgct caattttctc ctgatgataa tagccaaaat agtatgctcg aaggtgcgga
    13081 aagagtagca gagttatatc aaattgagaa agcagacttg gatttttggg taaaggaaag
    13141 tcataaaaga gcaaaagaag caagagagga aaaaatactt gaagatatta tatctcctat
    13201 aaataatagt acctatgatg aaggtatacg tgataagatg agtcaaaggt tattagatag
    13261 gatgccatca attttaggaa aagaaactat tacaaatgca gcaaattcat gtcttattaa
    13321 tgatggagct tcatttatta taatatgttc taagaagtat ttagaacatg taaaaaagaa
    13381 gcctaaagca aaaattatta acacatgcac gataggaaca gatgcaacct taagtccaac
    13441 atcagcgata caagcaatgg ataaactttt agagatagaa agccttaatt atttagatgt
    13501 atctgctgtt gaatttaatg aagcatttgc agttatagat gtgttgtttc aaagaaagta
    13561 tccagagtta atagatagat ataatatatt tggtggagct ttagcgtatg gacatccata
    13621 tggtgcttca ggtgcaatta ttgcattaca tcttttaaaa gcattagaaa agactaaggg
    13681 tagatatgga atttgctcta tagctgcagc tggtggtcta ggttcagctt tactgcttga
    13741 aagggtgtag attatgaact attatgaact tttaagaata aaaaaagaga ggcataaaaa
    13801 taaaaatttt ttaattatag atggagaaaa atatacttat gaaaatatat tgagttatag
    13861 tgaggaacta ggaaagcaaa tttctttagg tgaaaatatc ccaattttaa tatattctaa
    13921 aaatataatg tttcagttga ttagtttttt tgctattaat tattcaaaaa atattcctat
    13981 tatttgtcat tataatcttt ctaaaaaagt attagatgat attttattaa aaaataatat
    14041 ttcaatcatt atatctgatg aatctatgga cgttattgat ttatatgatg ataacaataa
    14101 tgaagtagca aaaaaagtta atttttctat ttttaatcct aaatttaata tatatatgta
    14161 tcaatataag aagcttataa attatttaga taaaagtatt tgtatgggag ctttatcttc
    14221 aggttctaca agtgttccaa aggtattata tagaacatat gagagttggg ctggattttt
    14281 tcctatacaa aatggtattt ttaatatctc tggggaatca atcttgtttg ttaatggaac
    14341 acttagtttt acagggaatc taaattctat aatgtcagta ttatatgaag gaggtagtat
    14401 tgtaataagt tcaggtctta attgtaaatc atggataagg acaataaaac aatataatgt
    14461 aactaacata taccttatac ctacaaaact tcaacttttg gttaaacact taaaagaacc
    14521 agtactcaaa gttgtaagta tttttacagg gtctcaatta ttatttgaag atactgcaag
    14581 aaatctaaaa aaatatatgc caaagtctga aattatttta tactatgggg caagtgaatt
    14641 aaattatatt acatatttat gctatgatga actaattgaa aaacctctaa gtgtaggcag
    14701 accatttcca ggaatagata tatatattag agaaggaaaa atttttgtga atacagaata
    14761 tgcagtatat aatgcaacca aaccttattc tgtcaatgat attggatatt atgataatga
    14821 tggatactta atatttgaag gaagaagtga tgacattata aatattggag gatttaaagt
    14881 tagttctact aagattgaaa atgaagttaa aaaaattcct caaattgaag atgcaattgt
    14941 attgccgtac tcagacacta taagaggaaa tcaaattgta ttatttgtaa taactataga
    15001 caaaattacg aagaaagatt tgttgattaa aatgaaagat aatctaatga aaaatgaaat
    15061 cccaaagaaa attatctttt taaattcatt tccatatacc tcttctgaaa agatagatag
    15121 attagcatta ttaaaaatgt tataagtata atatttagta attttagtga accaatgtat
    15181 tgataaaaac atattgtata tagtaaagga ggcttacaat atgtcaaatc aatacgatat
    15241 aactgttatt gggggagacc tgcgacaagt gtacatggta aaaaaactaa ttaataaagg
    15301 gtattctgta gtcacttatg ggatatcaaa tgaaattata aatggaatcg ttggaaaatc
    15361 aacttcacta gctgaagcac tatccaaaag ttccattatt ttatgtccaa ttccatttac
    15421 aaagaatcaa gttaatattg aaaatacgga tgattgtctt gatgctacta ttgataactt
    15481 gctaattaat ttatctccta atcaaatatt atttggaggt aacataccaa ctaaagtttg
    15541 taaattttgc gagcaacact ctattcagat ttatgatttt atgaaaatga atgatgttgc
    15601 tattttaaat gcagttgcta cagctgaagg ggctatagca gaagctatta aaagaagtat
    15661 aatcaatata catcaaagta actgtttagt attaggttat ggaagatgtg cacaaatact
    15721 tgcaaaaaaa ttatacgctt tggatgcaaa tgtatgtgtt ggcgcaagaa ataatgatgc
    15781 aagaagtacg gctaatgcat ttggatattc cagtatttct ttaagtcaac ttgatgaaaa
    15841 acttttacaa tttgattata tttttaacac cataccagag tgtattttaa cgaaagaaag
    15901 gttagaaaaa gtatcacaag aagtaactat tatagatata gcttcttctc ctggaggagt
    15961 agattattct gttgttaaag aagcagggat taatgcgact ctttgccttg ggcttcctgg
    16021 taaatattca ccaaagacat ctggtgaaat tcttgttaac gctatagaaa atattataaa
    16081 tgaaagaagg gattagaaat gagacttgaa ggacttacaa ttggagtagg atttactggt
    16141 tcgttttgta cttatgataa aatatttata gagttagaga atttggttaa agaaggagct
    16201 aatgtacaca ctatattttc agatgtatca caaaatatcg attgtagatt tggaaattct
    16261 gaagagttta tgaaaaaagc atatgaactt acaggaaata aacctatagt tacgattgaa
    16321 gaggcagagc catttggacc taaaggaatt gctgatatta taataatagc tccttgtact
    16381 ggaaatactg ctgcaaaatt agcaaatggt attacagatt caccagtatt aatggcagca
    16441 aaaggtcatc ttcgtaatga taaaccacta gtaatatcaa tatcaacgaa tgatgctcta
    16501 agctttaatt tcaaaaatat tggcattctc cttaattcta aaaatatata ttttgtacct
    16561 tttggtcaag ataattgcaa agcaaagcct aattctatga ttgcacatac tgagctaata
    16621 attccaacta tagaacttgc tctagagaac aaacagctac agcctgttat aaaaagtcca
    16681 catcaataga attggttctt ataatattat gtattagata caatactagc aaagaggctg
    16741 tctcaaaata gagagaagcc tctttttctt gttgaaatga tgaaaatata tttaatttca
    16801 tacaaaaaaa tgcctcatgg actaaaaagt ctattttgag ctaatctccg taatttttgc
    16861 tatattaaaa ttatttactt aatccaattt ttaaattaga atttatatcg ttttgactct
    16921 tcaaatatca atctaactgt tgttgataca aaagaagagg acataattaa aacaaatact
    16981 gatagtcctc taataaacaa attatctaca taataagtta ctattccaat acctccaatc
    17041 gttaaaatta cacttataat caaagaaaaa acgctaattg tttttaattt ttccatgctt
    17101 atcctccttc ttataaactt caaatttatc tatataatat taaatatatt ttatttcata
    17161 ttatataaaa atcatgaata tatat
//
LOCUS       DAMJVK010000087        17116 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.089, whole genome shotgun sequence.
ACCESSION   DAMJVK010000087 DAMJVK010000000
VERSION     DAMJVK010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17116
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.089"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            78..845
                     /locus_tag="PC414_002879"
     CDS             78..845
                     /locus_tag="PC414_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDF2465261.1"
                     /translation="MGEIINALDRALDIILLLYHEKREMGITEISKAMGVYKSTVHRT
                     LVTLENKGFVIQNAENGKYWLGINLYAIGMVVGEKMSLTEIVKPYTKKLNQEFNEVVN
                     VSILEERAQDSPRSIIIHKEYGSNQLLSVNPSVGSSSECYCSAVGKCLMAFNDSIDFE
                     KYRKTPIHKYTEHTIDNWDDMMLFLAKIKEQGYAIDDEELEHGLTCIGAPILDKNNKA
                     IAAISLSGPTIRMREGDFEYKIKRVIETAKSISELFR"
     gene            1128..1325
                     /locus_tag="PC414_002880"
     CDS             1128..1325
                     /locus_tag="PC414_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2465262.1"
                     /translation="MEKVKLSVERCKGCYLCIEACKKDAISLSDYTNKKGFVTIKVDE
                     EKCIQCGACYTMCPDLVFEIL"
     gene            1354..2430
                     /gene="vorB"
                     /locus_tag="PC414_002881"
     CDS             1354..2430
                     /gene="vorB"
                     /locus_tag="PC414_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HDF2465263.1"
                     /translation="MQSLLIKGNEALAEGAIRGGCRFYAGYPITPQSEILEMMAWRQK
                     EVGGVFIQGESEIASVNMVMGASALGARAMTSSSGPGFSLKQEGIAYWVSAGLPAVVA
                     CVQRYGIGDGFIGAGQDNYWQAVKGGGNGDYHLIVLAPNSVQENMDMAYESFELAEKY
                     MHPVLILSDATIGQMVEPCIAPPMKEYDMDKAPWAAKGTPVGEKNKVITDITYFEDQD
                     TWQKGYIEKMRKVQEDEQRWEEIEIEDAEVVFVAYGISSRIAEGAVRDARAKGMKLGL
                     IRPKTLWPYPRKAFKKLSDSLKGLVTVEMNMMAQMKEDVIIACDNRFPVYSVATAQYM
                     AEVNQLIDFAQQIIDGKAKQEEVF"
     gene            2433..3152
                     /locus_tag="PC414_002882"
     CDS             2433..3152
                     /locus_tag="PC414_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HDF2465264.1"
                     /translation="MAKKAPAIMMCENMFCGGCGHSIVDRIIGEVLTEMDICQDTIIC
                     SDIGCNHMFQFSMNVDVVVPPHGKMGAAMAAMKRVRPDKYIFTIAGDGGAYAIGLGET
                     MSAATRNDNVIFLVMNNNMFAMTGGQMAPTTIEGQYTASTPAGRNYADHGSTFDVVKV
                     MGNLDIAYLARGTITNPKEINTTKRYIKKALEKQKEKKGFCLIEVLVPCPTNWGLKPV
                     DAMKRIENELVPKYPLGEFID"
     gene            3165..3704
                     /locus_tag="PC414_002883"
     CDS             3165..3704
                     /locus_tag="PC414_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HDF2465265.1"
                     /translation="MLNEIICAGFGGQGVLTAGKIIMYVAYKAEKKITWFPSYGNEMR
                     GGSANCNVVVSDEKIANPCVKNPDILLGMNSVSVDKYESLMKKNGYMFVNESLVSKDR
                     KYREDINVIKVPVTEIAQNLGNEKAANICMLGAMVKSLDLFTKEEFESGMCEYFENQG
                     KGKFNSKNVEAFEAGYDYE"
     gene            3861..4919
                     /gene="buk"
                     /locus_tag="PC414_002884"
     CDS             3861..4919
                     /gene="buk"
                     /locus_tag="PC414_002884"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HDF2465266.1"
                     /translation="MKVLVINPGGTSTKISVFQDENEILKKNITHTREDLKNFSKVFD
                     EYDYRKQLIVDILSSENHSINSFDAVVGRGGLMKAIKGGTYTVSEEMIEDMRNEINGE
                     HASNLGALLAKTIADEIGVQSFVVDPVSVDEFDDVSRITGISDIEKASWLHALNHKAV
                     SRKIAQKLGGKYEDYNFIVAHLGSGISIVAHKQGKMIDGSGGRSDGPFSPERSGGLLA
                     YPLIELCYSGKYTHDEMVAKVSSIGGMYDYLGTKDMIEIENRIKEGDEKAKLIMDAFI
                     YQVAKEISMYGASLSGNVDRVILTGGISHSEMVVKGVAEKVSYLAPIEIVPGEMEMEA
                     LALGALRVLNGEEIAKTY"
     gene            4937..5830
                     /locus_tag="PC414_002885"
     CDS             4937..5830
                     /locus_tag="PC414_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HDF2465267.1"
                     /translation="MLKDFNNLLEEAKKLEPVIVSVAVAEDKEVLKAVKSATDLNIIK
                     PILVGDKDKIESISREIGLKGHEIIGCQNPDESVKKAVEIVKNKEAKVLMKGLVNTSV
                     YMRAILNKETGLRTGRLLSLLAVYELPQYHKLLYCTDSGINVSPNLNQKKDIMTNALL
                     TMKSIGMENPKTAILTANEMVDPKIQSTVDANLLVEMEKSGEIPKCIVEGPIAFDVAF
                     DAHAAEHKGIKSNIAGDVDLLVFPNIEAGNILGKSWLRFNKAKWAGIVLGATNPIILG
                     SRSDTAEIKINSIALACLASQ"
     gene            5860..7068
                     /locus_tag="PC414_002886"
     CDS             5860..7068
                     /locus_tag="PC414_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF2465268.1"
                     /translation="MKYAKRMDMVKASAIRDSQKKIAQKVASGGTVISFAAGLPDPNL
                     FPIEEISEITQKVLNERGKFALAYGPTKGEDELLDLLVKRMKEEENIDTKAENIIITT
                     GSQQGIALSAMILLEKGDVVVTENPSYLGAINAFRPYECDFLGVDTDEEGMVTDDLDK
                     LLSENSNIKVIYVIPTFQNPTGKTWSLQRRKDFMEVVNKYDVIVIEDNPYGEIRFTPE
                     PLPTLKSLDTKDKVLYLGSFSKVLCPGFRVAWMCGNVDIIDKAELLKQGIDLQSNQFS
                     QVQVIEFLKKYNLNEHIEKIRAEYKKRCLLMLDAMKKHFPEEAKYTEPDGGMFIWVEL
                     PESINVDDLLDKAIDAGVAYVSGESFFANNGPKNTMRLNYTTMSEEQIVKGVEILAEV
                     IKKELNYACE"
     gene            7092..8351
                     /locus_tag="PC414_002887"
     CDS             7092..8351
                     /locus_tag="PC414_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2465269.1"
                     /translation="MGEKENGIKFGKLMFQLVIIAISWELVYIIPFIQYTLYDPILKA
                     LECTNTQLGFLLTIYGLGNIFGAPIGGWLADRFDYRKIILGSVFLNGVVSFLFAFNMN
                     YPFAVATWVGCAITSLVMNYPSMVKILRVIGKDNQGKVYGFNEAMVGVSGVVMGAIFL
                     YIYTCFATPTLGMRWVMISLGILSIAMCPVLWFVIKDVDIKEEREEAGEKMSAGDFIT
                     VLKSPNTWLVGISIFCVYSFTVTMSYFTPYITSVLGGSVALSGALAIIRQHGLKLFGA
                     PFGGYCADKLKSPTKVLLPIYVFGIAVIVLFLVLPASTPMTIFIALTFVVGILGYMGK
                     GIYYAVQDEVKVPVKYSATTIGIAAALGFSPDVFQFALIGHWIDTYGNKGYTYTFIFQ
                     IAILVIGILSCLYILKVKKRRASKLEA"
     gene            8996..10459
                     /locus_tag="PC414_002888"
     CDS             8996..10459
                     /locus_tag="PC414_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HDF2465270.1"
                     /translation="MTDLVNTINGIVWSPVLVAMCLCIGLYFSLKLKFFQIRDVKEMF
                     TLLLEGDSSHVGISSFQGFATSMAGRIGTGNIAGVAVAIAMGGPGALVWMCIMATLGS
                     ATAFVESTLAQIYKDEHDGQYRGGPPYYFEKGLGWKKCSVLFSIVAIISYTFFMPGTQ
                     SNTLYLAMNQAFGFSKMTIAVASSIALAIIIFGGIKRIGLFAEKVVPIMGGAYLIMTL
                     VIIITNISLVPEAIVTMFKSAFGFGSVFGGMLGSAISWGVKRGIYSNEAGEGSGTYGA
                     AAAEVSHPAKQGLVQAFSVYVDTLLICMATGIMIVITGMYNVTGVCTALPGVEPGPQY
                     VQSAINTLHPQLGDIFIAIVLLLFTFTTLLAFSYMMETNVSYLVTRIKAEKHEKYIIN
                     VCRFMLIACAAMCCFIEPLTSWALGDICIGVLTYINLIAIFTLKKPAIKAYYDYIRQK
                     KMGIKRSDRTFNPIELDIRNAEFWENRYLKEDKHELN"
     gene            10515..11651
                     /locus_tag="PC414_002889"
     CDS             10515..11651
                     /locus_tag="PC414_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2465271.1"
                     /translation="MKERIIQVADSKKEKILGLCQSLYDEPEIALQEYKSAKKISEFL
                     REEGFDVEENLAGMATAFKATKKNGDGPKIAFIAEYDALPGNGHACGHHLIASMGVGA
                     GIALSSILDTYKGEVSIIGTPAEETGDGKPYLIEHGVFDGYDAAMMIHPNSKTCVTPE
                     IIAIGGLDFIFTGKASHAGAKPYNGINALDAVVLLYNNINALRQQLVDGTRIHGIILE
                     AGTAANVIPDMGKVRLEIRAKEQNYFDEVVEKVKNCARGAAIATGCELEFYHFEPTCQ
                     GLNENKVLVDIFTKIMEEFGIYEDEQVMLGSTDMGNLSQIMPCIHPLMKFSENGEELH
                     TKEFLEASINSYAKDRVIDGIKILALTGFNLFENPELLKKMKEE"
     gene            11887..13002
                     /locus_tag="PC414_002890"
     CDS             11887..13002
                     /locus_tag="PC414_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HDF2465272.1"
                     /translation="METLEVRNIELSGTNYEIGYRLGELVANMPEIIEGQINKSNLVS
                     KKEEKEMIELFDKYCPGLNEELQGFADAIQVNCNQILYYTMTYLKPGCSQVALAPELT
                     ENGHVLFARNFDFSHNMEDFVLCKTKVNGKYAHIGTTIMQFGRGEGMNECGLGVSQSS
                     CGIPVGNSDGLRKPAIVGLQFWAVIRYLLENCKDVDEALEYLKDMPIAYNINLLLADK
                     SGNIALVETLDGKKEVNIINSLESKREYFLHSTNHTHIDKLHKLDPQSMKNSIHRYKL
                     IKEYINKSKKIGEKELMNLLSSKYPNGLSCNYYNDFFGTLKSIVMDLNIGKFNILWGG
                     LENKWESYYLKNDIKSITQRININIEKAPSDFFDFIE"
     gene            13154..14023
                     /locus_tag="PC414_002891"
     CDS             13154..14023
                     /locus_tag="PC414_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2465273.1"
                     /translation="MDYLKYFEKAVMYIENNLHENITVNDVAKETGYSYYHLTRLFKS
                     MFGESVGSYIKKRRLVSSTKELLYSDKKVIDIAIGSGFESSEAFSRAFKSIYKVSPIE
                     YRKNRIDVFVGKKKKLELDFMRHLVGNITIKPVIKEIEKIKVIGIKDKVILENNSLPD
                     LWEKFRKVHHIVPNTLPSRRVFGICEAISEIHLVSESMEFNEIIGLEVNSYDVIPNSF
                     VSKTIKEGKYAIFTHTGSLDNLDKTYEYIWGTWFLSSKEELDIRDDFEVYDERFLGPS
                     NVNSQIDIYIPIK"
     gene            14215..15105
                     /locus_tag="PC414_002892"
     CDS             14215..15105
                     /locus_tag="PC414_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2465274.1"
                     /translation="MAKLTLEHIKGNTYYIPLPTIVGVYVDGKDAILIDSGNNKDTAR
                     QVLRLLEEHNLNPKLIINTHSNADHIGGNAYLKNQTKCKIATTKIEGYFTENPILESA
                     FLYGGYPSKALKNKFLLAKESEVDYIIPSNGKIVDTELEAISLPGHYFEMIGVRTPDN
                     VLFVADSLTPENIITKYHFFFLLDIDSQFKTLEKLRMLEADFFVPSHSVKTTDIKSLI
                     NINKKKMEEIIDNIKKVCYEPVMIDEVIEKMCDLYNVKLDANQYVLVGSTIRSYITYL
                     YENNMVEYIFDGGKMMIKAV"
     gene            15323..17077
                     /locus_tag="PC414_002893"
     CDS             15323..17077
                     /locus_tag="PC414_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp23"
                     /protein_id="HDF2465275.1"
                     /translation="MYIMNKKFKKFSKKLSILILVLIMFLFNNLTANAQIKEDSIIGI
                     DRYETAGLIADRQTYDTVILVNGDKNLSDGLSSSGLAGAINAPILLTKKSEIPKATLN
                     RLDNKSLNVVKKVYIIGGYNTIENSVEKDIKRKGIQVERINGNNRIETSYNVAKKINE
                     VSSVKEVMLTNGFVGEADAMSIAPVVAKNKGAIILTDGKSVPFKTESLNVYAIGGKSA
                     INEDLVNETKATRIGGNDRFETNKKVMEKFYNGATDFYITNGYQLVDALTLSPLAKEK
                     PIVLVADGSNKGILKGAKSITKVGGIDANIYKQCLDMIKYNNMDMTPNILKHLTSVEG
                     NEVSEILIESDNLGVVVEKTNTNKFEFDYVSVTNEKNCTFSVYKENSSNNIKNGKLVV
                     SAKKKIDKKDSDLQDMNGDNMINANKDRMVNVIKIGVPDKMYSNFKVDVESGSVELYN
                     IKGGATVDVNDGIANIVDNNVTNPFNINTNSGISGVTAETISSEIKFRANNGIVNVTA
                     TNINGNISLFGSDNKGTFDGIFKLNLKKEPSNLHLKLIGNGINKLPEGWSKDYILGNG
                     QPIIEVKNNGINNISIGE"
BASE COUNT         6718 a         1790 c         3260 g         5348 t
ORIGIN      
        1 aattttgtaa ttttaatata taatatttag tagagtgtgg gagtagcact taatatttga
       61 aggaggagtt tttgtatatg ggagaaataa taaatgcatt agatagagcc ctagacataa
      121 ttttgctgtt atatcatgaa aagagagaaa tgggaataac tgaaataagc aaggctatgg
      181 gagtatataa aagtactgta catagaactt tggttactct tgaaaacaaa ggttttgtta
      241 tacaaaatgc agagaatggt aagtattggc taggaataaa tttatatgca ataggtatgg
      301 ttgtgggaga aaaaatgtca ttgactgaaa tagtaaaacc atatacgaaa aaattaaatc
      361 aagaatttaa cgaagtagtt aatgtatcta tattagaaga aagagctcaa gattctccaa
      421 gaagtatcat aatacataaa gagtatggtt caaatcaatt actttcagta aatccatccg
      481 ttggttcaag tagtgaatgt tattgttcag cagttggtaa atgtcttatg gcatttaatg
      541 attctataga ttttgaaaaa tatagaaaaa caccaataca caagtataca gaacatacaa
      601 ttgataattg ggatgatatg atgctttttt tagcaaaaat aaaagaacaa ggatatgcta
      661 tagatgatga agaactagaa catggactaa cttgtattgg agcacctatc ttagataaaa
      721 ataataaagc tattgcagcc ataagtttgt caggtccaac tataagaatg agagaagggg
      781 actttgagta taagattaaa agagttatag aaactgcaaa aagtatatct gaacttttta
      841 gatgattttt aatctcgata ataaaaatag gctatattta aattgttatt tgtaaatatc
      901 agtttaaatg tagcctattt ttctttaatc taaaattaaa tattcttaaa atttaaaatg
      961 ttattgaaat atataagcga atgtgttatc ttttaattaa gcaataaaaa tagaacaagg
     1021 ttctatacta tagaacaaaa ttgatgcata tatagtattt atacaaaata taaatattat
     1081 atatttaaaa ttaagtaaaa atattacata aaggggagat ttgagaaatg gaaaaagtca
     1141 agttgagtgt tgaaagatgt aagggatgct atctttgtat tgaagcttgt aaaaaagatg
     1201 caataagtct ttctgactac acaaataaaa aaggctttgt aactattaaa gtcgatgaag
     1261 aaaaatgtat tcaatgtggc gcttgttata caatgtgccc tgatttagta tttgagatat
     1321 tataaattat ttttattaga ggggagaaaa ggtatgcaaa gtttattaat taaaggaaat
     1381 gaagctttag cagaaggtgc tatacgtggt ggctgcagat tttatgcagg ttatccaata
     1441 acgccacaat cagaaatatt agaaatgatg gcatggagac aaaaagaagt tggaggagta
     1501 tttatccaag gtgaatcaga aatagccagt gtaaatatgg ttatgggagc aagtgcactt
     1561 ggagcaagag ctatgacttc ttcatctggt ccaggatttt cattaaaaca agaaggtatt
     1621 gcatattggg tatcagctgg tttgccagca gttgtagcat gtgtgcaaag atatggtata
     1681 ggtgatggat ttataggagc tggtcaagat aattattggc aagcagtaaa aggcggtgga
     1741 aatggagatt atcatttaat cgtgttagca cctaatagtg ttcaagaaaa tatggatatg
     1801 gcatatgaat cttttgaatt ggctgaaaaa tatatgcatc cagtacttat actttctgat
     1861 gctacaatag gtcaaatggt agaaccttgt atagcaccac caatgaaaga atacgatatg
     1921 gataaagctc catgggctgc aaaaggaaca cctgtaggag aaaaaaataa agtaataact
     1981 gatataacat attttgaaga ccaagataca tggcaaaaag ggtatataga aaaaatgaga
     2041 aaagttcaag aagatgaaca aagatgggaa gagatagaaa tagaagatgc tgaagtggta
     2101 tttgtggcat acggtatttc atcaagaata gcagaaggtg cagttagaga tgctagagca
     2161 aaaggaatga aattaggtct aataagacca aaaacattgt ggccttaccc aagaaaagca
     2221 tttaagaaat taagtgattc attgaaggga cttgtaacag ttgaaatgaa catgatggca
     2281 cagatgaagg aagatgtaat tatagcatgt gataatagat ttccagtata ttcagtagca
     2341 acagctcaat atatggcaga agtaaatcaa ttaatagatt ttgctcaaca gattatagat
     2401 ggaaaagcta agcaagagga ggtattttaa ttatggctaa aaaagcacca gcaataatga
     2461 tgtgtgaaaa tatgttctgt ggaggctgtg gacactctat agtcgataga attattggag
     2521 aagtattaac agaaatggat atatgtcaag atacaattat atgttcagat ataggttgta
     2581 atcatatgtt tcaatttagt atgaatgtag atgttgtcgt tccacctcat ggtaaaatgg
     2641 gagcagcaat ggcagctatg aaaagagtta gaccagataa atatatattt acaatagctg
     2701 gagatggtgg agcttatgca atagggcttg gagaaactat gtcagctgct actagaaatg
     2761 ataatgtaat atttcttgtt atgaacaata atatgtttgc aatgacagga gggcaaatgg
     2821 ctccaactac tatagaaggt caatatacag cttctactcc agcgggtaga aattacgcag
     2881 atcatggttc aacatttgat gttgtaaaag ttatgggaaa tttagatata gcatatctag
     2941 caagaggaac aataactaac cctaaagaaa taaatacaac aaaaagatac atcaaaaaag
     3001 ctctagaaaa acaaaaagaa aagaaaggtt tttgcttaat agaagtgctt gtaccatgtc
     3061 caacaaactg gggattaaag ccagtagatg ctatgaagag aattgaaaat gaacttgttc
     3121 caaagtatcc acttggagaa tttattgatt agggaggaaa taatatgttg aatgaaatta
     3181 tatgtgctgg ttttggagga cagggagttc ttacagcagg aaaaattata atgtatgttg
     3241 catataaggc agaaaagaaa attacatggt tcccatctta tggaaatgag atgaggggag
     3301 gaagtgcaaa ttgcaatgtt gttgtaagtg atgaaaaaat tgcaaatcct tgtgtaaaaa
     3361 atccagatat actattggga atgaacagcg tatcagtaga taagtatgaa agtttaatga
     3421 aaaaaaatgg ttatatgttt gtcaatgaat cactagtaag taaggataga aagtacagag
     3481 aagacattaa tgttataaaa gtacctgtta ctgaaatagc acaaaattta ggcaatgaaa
     3541 aagctgctaa catttgcatg ttaggtgcta tggtaaagag tttggattta tttacaaaag
     3601 aagaatttga aagtggtatg tgtgaatact ttgaaaatca aggcaaaggt aaattcaatt
     3661 ctaaaaatgt agaagcattt gaagctggat acgactacga atgaatattt taaatattta
     3721 aaataattat tgaaatactt atttttatat gttagtattt aattaataaa atatagaacg
     3781 tcgttctgga caatgggacg aagtctttag gtcggaatac aagaacggcg ttctgtataa
     3841 tagaactagg gggtgttaaa ttgaaagttt tagtaataaa tcctggtggt acatccacaa
     3901 aaatttctgt tttccaagat gaaaatgaaa ttttgaaaaa aaatataacc catacaagag
     3961 aagatttaaa aaatttttct aaagtatttg atgaatatga ttatagaaaa caactgatag
     4021 tagatatttt aagtagtgag aatcatagta taaattcttt tgacgcagta gttggtcgtg
     4081 gtggacttat gaaggctata aaaggtggta cctatactgt gagtgaagaa atgattgagg
     4141 atatgagaaa tgaaataaat ggggaacatg catcaaattt aggtgcatta ttagctaaaa
     4201 ctatagctga tgaaatagga gtacaatcat ttgtagtaga cccagtttct gtagatgaat
     4261 ttgatgatgt atcgaggatt acaggaatat cagacataga aaaagcaagt tggttacatg
     4321 cattaaacca taaagctgtt tcaagaaaaa ttgcacaaaa actaggggga aaatacgagg
     4381 attataactt tattgtagct catttaggtt caggaatatc gattgtagca cataaacaag
     4441 gcaaaatgat tgatggaagt ggtggtaggt ctgatggtcc attttctcca gaaaggagtg
     4501 gtggattact tgcatatcct cttatagaat tgtgttactc cggaaaatat actcatgatg
     4561 aaatggtcgc aaaagtaagt tcgattgggg gaatgtacga ttatttagga acaaaggaca
     4621 tgatagaaat tgaaaacaga atcaaggagg gtgatgaaaa ggcaaaactt ataatggatg
     4681 catttattta tcaagtagca aaagaaattt ctatgtatgg agcttcactt tcagggaatg
     4741 tagatagagt tatcttgaca ggagggatat ctcactcaga aatggtagtc aaaggagtag
     4801 cagaaaaagt ttcatatcta gctccaatag agatagtacc aggagagatg gaaatggagg
     4861 cacttgctct aggggcactt agagtactta atggagaaga aatagcaaaa acttattaat
     4921 atttaggaga aagagtatgt taaaagattt taataattta ttagaagagg ctaaaaagct
     4981 tgaacctgtt attgtaagtg ttgctgttgc agaagacaaa gaagtattaa aggctgtgaa
     5041 atcagcaaca gatttaaaca ttataaagcc aattcttgta ggagacaagg ataaaataga
     5101 aagtatatca agagaaattg gattaaaagg tcatgaaata ataggttgtc aaaatcccga
     5161 tgaatcagtt aaaaaagctg tagagatagt taaaaataag gaagctaaag ttcttatgaa
     5221 gggattagta aatacaagtg tctatatgag agctatatta aataaagaaa cgggtcttag
     5281 aacaggaaga ttattaagtt tgcttgcagt atatgaatta cctcaatatc ataaacttct
     5341 ttattgtact gatagtggaa taaatgtatc gccaaactta aatcaaaaaa aagatataat
     5401 gacaaatgca cttcttacta tgaaaagtat aggcatggaa aatcctaaaa ctgcgatact
     5461 tactgcaaat gaaatggtag accctaagat acaatctaca gtggatgcca acctacttgt
     5521 agagatggaa aaatcgggag aaataccaaa gtgtatagtg gaaggtccaa tagcatttga
     5581 tgtagcattt gatgcacatg cagcagaaca taaaggtatt aaaagtaata ttgctggtga
     5641 tgttgattta ttagtatttc caaatataga agcagggaat atattaggaa agtcatggct
     5701 tagatttaat aaagctaagt gggcaggtat agttttagga gcaacaaatc cgataatttt
     5761 aggttcacgt tctgacacag ctgaaataaa aataaattca atagcattag cgtgcctagc
     5821 atcacaataa gaaattatta ttaaggagga aaaagtatta tgaaatatgc aaaaagaatg
     5881 gatatggtaa aagcttcagc tataagagat tctcaaaaga aaatagctca gaaggtagca
     5941 agtggtggaa ctgttatatc ttttgctgca gggcttcctg acccaaattt atttccaata
     6001 gaagagataa gtgaaataac tcaaaaagta cttaatgaaa ggggaaaatt tgccctagca
     6061 tatggaccta ctaaaggtga agatgagttg ttagatctat tagtaaaaag aatgaaagaa
     6121 gaagaaaata ttgatacaaa agcagaaaat attataataa ctactgggtc acaacaaggg
     6181 atagcattaa gtgctatgat tttattagaa aagggagatg tagtagttac agaaaatcca
     6241 agttacttag gtgcaataaa tgcatttaga ccttatgaat gtgatttttt aggagtagat
     6301 acagatgaag aaggaatggt aacagatgat ttagacaaat tattatctga aaattctaac
     6361 ataaaggtga tatatgtaat accaacattt caaaacccta caggaaaaac atggtcttta
     6421 caaagaagaa aagattttat ggaagtcgtg aataaatatg atgtcatagt aatagaggat
     6481 aatccttatg gagaaataag atttacacca gaacctctac ctacacttaa atctcttgat
     6541 actaaagata aagtattgta ccttgggtca ttttcaaagg tattatgtcc aggatttaga
     6601 gttgcttgga tgtgtggtaa tgtagacatc atagataagg cagaactttt aaaacaaggt
     6661 atagatttac agtcaaatca attttcgcaa gtacaagtaa tagaattttt aaagaaatac
     6721 aacttaaatg aacatataga aaaaataaga gcagagtata agaaaagatg tctgctaatg
     6781 ttagatgcta tgaagaaaca tttcccagag gaagctaaat atacagaacc agatggagga
     6841 atgtttatat gggttgaact tcctgaaagt ataaatgtag atgatttatt agataaagcc
     6901 attgatgctg gagttgctta tgtttctgga gagtccttct ttgcaaataa tggtccaaaa
     6961 aatactatga gacttaacta tacaacaatg tcagaagaac aaatcgttaa aggtgttgag
     7021 atacttgcag aagttataaa aaaagaactc aattatgctt gtgaataaat ataaaaagtg
     7081 agggtttaaa tatgggagaa aaagaaaacg gtataaagtt tggaaagtta atgtttcaac
     7141 ttgttattat agcaatatct tgggaattag tttatattat accatttatc caatatacat
     7201 tatatgatcc aatcctaaaa gcattagaat gtacaaacac tcaactaggt tttctactta
     7261 caatttatgg gttaggtaat atatttggag ctcctatagg aggctggtta gcagatagat
     7321 ttgattatag aaagataatt ttaggttctg tatttcttaa tggagtagta tcatttttat
     7381 ttgcatttaa tatgaactat ccatttgcag ttgctacttg ggtaggttgt gcaatcacat
     7441 cattagttat gaactatcct tctatggtaa aaatacttag agttatagga aaagataatc
     7501 aaggtaaggt ttatggattt aatgaagcta tggttggagt ttctggagta gtaatgggag
     7561 caatattctt atatatttat acatgctttg caactcctac attgggtatg agatgggtaa
     7621 tgatatcttt aggaatatta tcaatagcaa tgtgtccagt attatggttt gtaattaaag
     7681 atgtagatat taaagaagaa agagaagaag caggtgaaaa aatgtctgct ggtgatttta
     7741 taacagtact aaaatcacca aatacttggc tagtaggaat aagtatattc tgtgtatatt
     7801 cattcacagt tacaatgtct tattttacac catatataac atctgtactt ggaggttctg
     7861 tagcattatc tggtgcatta gcaataataa gacagcatgg tctaaaatta tttggagcac
     7921 catttggagg atattgtgca gacaaattaa aatctcctac aaaagtactt ttaccaatat
     7981 atgtatttgg tatagcagta atagttttat tcctagtatt acctgcatca actccaatga
     8041 ctatattcat agctttaaca tttgtagtag gtatattagg ttacatggga aaaggtatat
     8101 attatgcagt acaagatgaa gtaaaagttc cagttaaata ttcagcaact actataggta
     8161 tagctgcagc tttaggattt tcaccagatg tattccaatt tgcattaata ggacactgga
     8221 tagatactta tggcaataaa ggttacacat atacatttat attccaaata gcaattctag
     8281 ttattggaat actatcttgt ttatatatat taaaggttaa aaagagaaga gcaagtaaat
     8341 tagaagctta aatattcaat ttggtaatta agtatatata aatacaatac ttattttgat
     8401 gaatgtgatt gtataaataa cataaaaaat atcaatagat taagttttat aataatgttt
     8461 agagaataaa gtataaaaat caaaatgtta tcaagttttc taggttttaa cataaagtta
     8521 cccctgaagg tgtaaaattg agtggcaatt attatctatt ttcttaccta cataaaccta
     8581 tttaatttaa attctaagag attttcaaaa tagatggtag ttgtactcaa ttttaaacct
     8641 cataataaat acgaaaattt aaaatctaac tagatactat ttaattatat attaataaat
     8701 atatattgta ctatttatat aataataatt tcaggctttt cattatatgt attccaattt
     8761 atattgtttg attattattc aaacacacgt cgttaaaaat tttataaatt tatgtttaga
     8821 tatgattaat actcttgatt ttagaaataa atatttgtag attgtagatt aaagataata
     8881 taaataaata gaaggtagat tttttagata tattaatata aagattatat aaaatattga
     8941 aatatagctg tttaaattat tgtctataaa tataaaatga atggtggtga gttaagtgac
     9001 cgatttagtt aacaccatta atggaattgt ttggtcaccc gtattagttg caatgtgttt
     9061 gtgtatagga ttatattttt cattgaaatt aaaatttttt caaataagag atgtaaaaga
     9121 aatgtttact ctcttgcttg agggagatag ttctcatgtt ggtatatcat catttcaagg
     9181 ttttgctaca tctatggctg gaagaatagg gactggaaat atagctggtg tagctgtagc
     9241 aatagcaatg ggaggtccag gagcattagt atggatgtgt ataatggcta cattaggttc
     9301 agcaactgca tttgtagaat ctactttagc tcaaatatat aaagatgagc atgatgggca
     9361 gtatagagga ggacctccat attattttga aaaaggtctt gggtggaaaa agtgttcagt
     9421 tttattttca atagttgcaa taataagtta tacatttttt atgcctggta cacaatcaaa
     9481 cactttatat ctagcaatga atcaagcatt tggattttca aaaatgacaa tagcagtagc
     9541 atcttctatt gcattagcga taataatttt tggaggaatt aaaaggattg gtttatttgc
     9601 tgaaaaagta gtacctataa tgggtggagc ataccttatt atgacattag taataattat
     9661 aacaaatatt agtttagttc cagaggctat tgtaacaatg tttaaatctg catttggatt
     9721 tggttcagtt tttggaggaa tgctaggttc agcaatatct tggggagtaa aaagaggtat
     9781 atactcaaat gaagcaggag aaggttctgg gacttatggt gcggcagcag cagaagtaag
     9841 tcatccagcc aaacaaggat tagttcaagc attttctgtt tatgtagaca cattattaat
     9901 atgtatggct acaggtatta tgattgtaat aacaggaatg tataatgtta caggtgtttg
     9961 cacagcacta ccaggtgtag aaccgggacc acaatatgta cagagtgcaa taaatacatt
    10021 acatccacaa ttaggtgata tttttatagc aatagtttta ttattattta catttacaac
    10081 tttattagcc ttctcatata tgatggagac taatgtatct tacttagtaa ctagaattaa
    10141 agcagaaaaa catgaaaagt acataattaa tgtctgtaga tttatgctta tagcatgtgc
    10201 agcaatgtgt tgttttatag aaccacttac aagctgggca ttaggtgata tatgtatagg
    10261 tgtattaaca tatataaatt taattgccat ctttacacta aaaaaaccag ctataaaggc
    10321 atattatgat tatataagac agaagaagat gggaataaaa aggagtgata gaacttttaa
    10381 ccctatagag ttagatataa gaaatgctga gttttgggaa aatagatact taaaagagga
    10441 taagcatgag ttaaattaaa gttataataa aatataaaaa ttactaaata aatttagtgg
    10501 gaggtaagat tggtatgaaa gaaagaataa tacaagtggc tgattcaaag aaagaaaaaa
    10561 ttctagggct atgtcaatct ttatatgatg aaccagaaat tgctttgcaa gaatataaat
    10621 ctgctaaaaa aatatcagaa tttctaagag aagagggttt tgatgttgag gaaaacttgg
    10681 caggtatggc tacagctttt aaagcaacca agaaaaatgg agatggtcct aaaatagcat
    10741 ttatagcaga atatgatgct cttccaggca atggacatgc atgtggacat catttaattg
    10801 catctatggg agttggagct ggaatagctc tttcaagtat actggatact tacaagggag
    10861 aagtaagtat aattggaaca cctgctgaag aaactggaga tggaaagcct tacttgatag
    10921 aacatggtgt atttgatggc tatgatgcag caatgatgat acatccaaat tcaaaaactt
    10981 gtgtaacacc tgaaattata gcaataggag gtctagattt catatttact ggaaaagcat
    11041 ctcatgcagg agctaagcca tataacggaa taaatgcttt agatgcagta gtactactat
    11101 ataataatat aaatgcacta agacaacaat tagtagatgg aacgagaata catggtatta
    11161 tattagaagc aggaactgct gcaaatgtaa tacctgatat gggaaaagta agattagaga
    11221 taagggcaaa agaacaaaat tactttgatg aagtagtaga aaaagtgaaa aattgtgcta
    11281 gaggtgctgc aatagccact ggatgtgaac ttgaatttta tcactttgaa ccaacttgcc
    11341 aaggattaaa tgaaaataaa gttttggtag acatatttac aaagattatg gaggaatttg
    11401 gaatatatga agatgaacaa gtaatgttag gttcaacaga tatgggaaat ttaagtcaaa
    11461 taatgccatg tatacatcct ttgatgaagt tttctgaaaa tggagaagaa ctacatacaa
    11521 aagaattttt ggaggcttct ataaattcat atgcaaaaga tagagtaatt gatggtataa
    11581 aaatattagc tttaacagga tttaatttat ttgaaaatcc agaattattg aaaaaaatga
    11641 aggaagagta gaacttagac tatttttaat tatactcttt aaaataagaa gagtgtctca
    11701 aaatgaactt tttagtttat gagacactct tttttgtatg aaattaaata tatttttatt
    11761 attttaaaca tgaaaaagag gcttatctgt ttttatatga ccttttttaa cttgtagcac
    11821 aaaatatcat gaatgattac aattcgaatg ataaaatcga ttataagata tataggaggg
    11881 gtacatatgg aaactttaga agtaagaaat attgaattga gtggtacaaa ttatgagata
    11941 ggatacagac taggagaatt ggttgctaat atgccagaga taatagaagg tcagattaat
    12001 aaatctaatc tggtaagcaa aaaagaagaa aaagagatga tagagttgtt tgataaatat
    12061 tgtccaggtc ttaatgaaga attgcaaggt tttgctgatg caattcaagt gaattgtaat
    12121 caaattttat attatactat gacttatctt aaaccaggtt gtagtcaagt tgcattagca
    12181 ccagaattaa ctgaaaatgg gcatgtacta tttgcacgta atttcgattt ctcacacaat
    12241 atggaggatt ttgttttatg taaaactaag gtaaatggaa aatatgcaca tataggtact
    12301 accattatgc aatttggaag aggtgaaggt atgaatgaat gtggcttagg tgtaagtcag
    12361 tcatcatgtg gtattcctgt aggcaattca gatggtttaa gaaaaccagc aattgtaggt
    12421 ttacagtttt gggctgttat tagatatttg ttagaaaatt gcaaagatgt agatgaagca
    12481 ttagaatatt taaaagatat gccaattgca tacaatatta atttattgct tgcagataag
    12541 tctggaaaca ttgcacttgt ggagacttta gatgggaaaa aagaagtaaa tataatcaac
    12601 agtttagaaa gtaaaagaga atatttttta cactctacta atcatacaca tattgataaa
    12661 cttcataaat tagatccaca atcaatgaaa aattctattc atagatataa attaattaaa
    12721 gaatatataa ataagtctaa aaaaattgga gagaaagagt taatgaattt attgtcttca
    12781 aaatatccaa atggcttatc ttgtaattac tataatgatt tttttggaac tttaaaaagt
    12841 atagttatgg atttaaatat aggaaagttt aatattttat ggggtggact agagaataaa
    12901 tgggagagct actatttaaa aaatgacatt aaatctataa cacagaggat aaacataaat
    12961 attgaaaaag caccatctga cttttttgat ttcattgaat aaaaaatgta tttaattatt
    13021 ctaaataatt aggacatata aatcaactaa aagtttttat ttatcgtatt gaattagtta
    13081 taatatttta gtatagtaaa aaatagtgct attttacata tttaaagagt ttaagtagaa
    13141 gagaggtgcc atattggatt acttaaagta ttttgaaaaa gctgtaatgt atattgagaa
    13201 taatttacat gaaaatataa ctgtaaatga tgtggcaaaa gaaactggat attcttatta
    13261 tcatttaaca cgcttattta agtctatgtt tggtgaaagt gtaggaagtt atataaaaaa
    13321 aagacgatta gtaagtagta caaaagaact tttatatagt gataaaaaag tgatagatat
    13381 agctattggt agtggatttg aatcttcaga agcatttagc agagcattta aatctatata
    13441 caaggttagc cctatagaat atagaaaaaa tagaattgat gtatttgttg gtaaaaagaa
    13501 aaaattagag ttagatttta tgagacatct tgttggaaat ataacgatta aaccagttat
    13561 aaaagaaata gaaaaaataa aggtaatagg tataaaggat aaagtaattt tagaaaataa
    13621 ttcattacct gacttgtggg agaaattcag aaaagtacat catattgtcc caaatacttt
    13681 gccatcaagg agagtttttg gaatctgtga agcaatctct gaaatacacc tagttagtga
    13741 aagtatggag tttaatgaga taataggact tgaagttaat agttatgatg tgattccaaa
    13801 ttcatttgtt tcaaaaacta ttaaggaagg aaagtatgct atatttactc atactggtag
    13861 cttagataat ttagacaaaa cttatgaata tatatggggt acttggtttt taagttcaaa
    13921 agaagaactt gatataagag atgattttga agtatatgat gaacgatttt taggtcctag
    13981 taatgtgaac tcacaaattg atatttatat acctataaaa taatctataa gaaaactaaa
    14041 agataatttg attaagaaat agatgttcgt aaaagagcat ctatttttta tttttataag
    14101 atttagataa ttttaataat aaaagaaata aaaatatatt ataaaaacaa tttgattgga
    14161 attttcagaa aattaatgct ataattaaaa gtaaatttaa agggaggtta taaaatggct
    14221 aaattaactc tagaacatat aaaaggaaat acatattata ttccactccc tacaatagta
    14281 ggtgtatacg tagatggtaa ggatgcaatt ttaattgaca gtggaaataa taaggataca
    14341 gctagacaag ttttaagatt attagaagaa cataatttaa atcctaaatt aataataaac
    14401 actcattcaa atgcagacca tataggagga aatgcatatt tgaagaatca aacaaaatgc
    14461 aagattgcta cgactaaaat tgaaggatat tttacagaaa atccaatatt agaatcagca
    14521 tttttatatg gtggctatcc aagtaaagca ctgaagaata aatttttatt agcaaaggaa
    14581 agtgaagttg attatattat cccttcaaat ggcaaaattg tagatacaga acttgaagcg
    14641 atttctcttc caggtcatta ttttgaaatg attggtgtta gaactccaga taatgttttg
    14701 tttgttgcag atagtcttac accagagaat ataattacta aatatcattt tttcttccta
    14761 ctggatatag attcacagtt taagacacta gaaaaattga gaatgttaga ggctgatttc
    14821 tttgttccaa gtcactctgt caaaactaca gatataaaaa gtttaattaa tattaataag
    14881 aagaagatgg aagaaataat agataatatt aaaaaggttt gttatgagcc agttatgatt
    14941 gatgaagtta ttgaaaaaat gtgtgatttg tacaatgtca agcttgatgc taatcaatat
    15001 gtgttggttg gaagtacaat acgttcttat ataacttatt tatatgagaa taatatggtt
    15061 gaatatatat ttgatggtgg taagatgatg ataaaagctg tgtaaatagt aataattttc
    15121 aaattatatg atgaaattgt tttagtatga ctgttatttg aaaagtaata taaaaaaatt
    15181 tatataaata agctaagatg attgatataa agttactgat aattttaagg taatatatac
    15241 gttactataa tattatgtta ttatttgtaa ataaggggat aatttataag caaattaaag
    15301 accttaattt acaggaggaa tagtgtatat tatgaataaa aaattcaaaa aattcagtaa
    15361 aaaactaagt atattaattt tagttttgat tatgtttctt tttaataatt taactgcaaa
    15421 tgcacagatt aaagaagatt caataatagg aattgataga tatgaaacag caggacttat
    15481 agcagataga caaacttatg atactgtaat attagttaat ggagataaaa atttatctga
    15541 tgggctttca tcaagtggat tagctggagc tattaacgca ccaatacttt tgactaaaaa
    15601 aagtgaaata ccaaaagcta ctttaaatag actagataat aagagtttaa atgttgtaaa
    15661 aaaagtatat ataattggtg gttataatac tattgaaaat tcagtagaaa aagatataaa
    15721 aagaaaaggt attcaagtgg aaagaataaa tggtaacaat agaattgaaa ctagttataa
    15781 tgtagctaaa aaaataaatg aagtaagtag tgtaaaagag gtaatgctta caaatggatt
    15841 tgtaggagag gctgatgcta tgagtatagc acctgtagtt gcaaaaaata aaggagctat
    15901 aatacttaca gatgggaaat cagttccatt taagactgaa agtttaaatg tttacgctat
    15961 aggtggcaaa tctgcgataa atgaggattt agtaaatgaa actaaagcaa ctagaatcgg
    16021 aggaaatgat agatttgaga ccaataaaaa ggtaatggaa aagttttata atggagcgac
    16081 agatttttat ataacaaatg gatatcaatt agtagatgca ctaacattat ctcctttagc
    16141 caaagaaaaa ccaatcgttt tagttgctga tggaagtaat aaaggtattt taaaaggagc
    16201 taaaagtata acaaaagttg gaggtattga tgccaatata tataaacagt gtttagatat
    16261 gattaaatat aataatatgg atatgactcc aaatatttta aaacacttaa cttcagttga
    16321 aggaaatgaa gtttctgaaa tacttataga atcagataat ttaggtgtgg ttgttgaaaa
    16381 gacgaatacc aataaatttg agtttgatta cgtaagtgta actaatgaaa aaaattgtac
    16441 tttttctgta tataaagaaa attcttctaa taatataaaa aatggaaagc tagttgtaag
    16501 tgctaagaaa aaaatagata agaaagatag tgatttgcaa gatatgaatg gcgacaatat
    16561 gataaatgca aataaagata ggatggtaaa tgtgataaaa attggagttc ccgataaaat
    16621 gtattctaat tttaaagtag atgttgaaag tggttcagta gaactttata atattaaagg
    16681 aggagctact gttgatgtca atgacgggat tgctaacatt gttgataaca atgtaacaaa
    16741 tcctttcaat ataaatacta atagtgggat ttcaggtgta acagctgaaa ctatatcatc
    16801 tgaaataaaa tttagagcaa acaatggaat tgtaaatgta acagctacga atataaatgg
    16861 caatataagt ttatttggaa gcgataataa aggtaccttt gatggaatat ttaaattaaa
    16921 tctgaaaaaa gaaccatcaa atttacactt gaagttaatt ggaaatggga taaataaatt
    16981 gccagaaggt tggtctaaag attatatttt aggaaatgga caaccaatta ttgaagttaa
    17041 aaataatggc ataaataata tatcaattgg agaataaaaa catatgaaaa taaagtttat
    17101 atagttttaa aaatta
//
LOCUS       DAMJVK010000088        16902 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.090, whole genome shotgun sequence.
ACCESSION   DAMJVK010000088 DAMJVK010000000
VERSION     DAMJVK010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16902
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.090"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            160..1008
                     /locus_tag="PC414_002894"
     CDS             160..1008
                     /locus_tag="PC414_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDF2465276.1"
                     /translation="MNNQKNLNIPITISSYTLGTEVSFRDRVRIAKEAGFDGIGLRAE
                     NYIDAKNAGVTDEEMIAILNEYDMKVTEVEYITQWGREEDRTEAQREKEQNVYHMAHL
                     FNVKHINCGLLEMIPEEQIITALGELCDRAGELIIGLEFMPYSGVPDLATAWRVVKGC
                     NRENAMLILDTWHWARAKQTAEDLAPVPADKIVSIQVCDVLETPYEKLRDESLHDRLA
                     PGEGYGNTEEFVKIIKEHNISPRVIGAEVISDSLISKGLSTAAETVFNATKKVLDKAW
                     SEVSPK"
     gene            1072..2994
                     /locus_tag="PC414_002895"
     CDS             1072..2994
                     /locus_tag="PC414_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDF2465277.1"
                     /translation="MKISSMFTPIRIGSMTVPNRFVVPPMGNNFANTDGTLSETSKAY
                     YLERALGGFGLITIESSVVDKKAKGGPRKPCLYDDSTIDSFKNVIDACHDAGSKVSIQ
                     LQHAGSEGNEKVAGHPLKAASAIPASNGRNTPLAITTEEIYELIESYGDAALRAQKAG
                     ADAVEVHCAHGYLVSSFISQRTNKRVDEFGGCFENRMRLPRLIIENIRKKVGNSLAIL
                     CRINSTDDVPGGIDVHDSSVIAAYLEDCGIDGLHVSRSIHIHDEYMWAPTTLHAGFSA
                     ELVTEIKKAVSIPVITVGRYTEPQFAELMVRQGRCDLVAFGRQSLADPEMPNKAKNGK
                     LDEMIPCIACLQGCVPNMFQGKPIACLANPILGHEAELKPAEISKEVLVVGGGVGGML
                     AAWVCAKRGHNVTLVEKSEVLGGQMRLAAYPPGKGDITNLVRSYISKCNQYGVKICTN
                     TEATVELIKEKSPDVVIIATGATPLVLPIPGINDSGLIHAVDLLDGKKSCGKKVLVVG
                     GGMVGCEVAAFLGEQEHEVTVIELREEVGADVISEHRKFLMNDFENYNIHTITGAKVS
                     KFYDDGVDYLLTDGTEHDLKGFDSVVLAMGSRNYDPLSEVIKEVVKETYIVGDAVKAR
                     RALDATKEAFEVALNI"
     gene            3076..3939
                     /gene="aroE"
                     /locus_tag="PC414_002896"
     CDS             3076..3939
                     /gene="aroE"
                     /locus_tag="PC414_002896"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDF2465278.1"
                     /translation="MEISGRTGLFALIGTPVGHSKSPVMYNYSFKKLDLDYRYLAFDI
                     TVDKVKEALLAIKTFNIKGANVTMPCKSAVTEYMDELSPAARIIGACNTIVNDNGKLV
                     GHITDGVGYVRNLKENGVEVKGKKITIMGAGGAATAIQVQCALDGAREISIFNPKDDF
                     YKRAEQTVENIKKDVPECVVNLYDLEDTNKLYEEIQSSDILTNATLIGMKPYDNETNI
                     KDTSVLRKDLVVTDVVYNPKKTKMIEDAEANGCKAIGGLGMLLYQGAEAFNLYTGLEM
                     PVEEVNELCFK"
     gene            4139..5356
                     /locus_tag="PC414_002897"
     CDS             4139..5356
                     /locus_tag="PC414_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2465279.1"
                     /translation="MNNKKYYPTAIALYFSYFLLGIGISILGQYKPEFSSMWGAKTLS
                     DGTLDVSIVLAVIAALGLGRLISYPFAGPISDKYGRKVSGLIGNFLHAIFFVGIVFSP
                     NFYIAYVFAIIGGAANSFLDTCVTPSCMEIFASLGTIANMFTKFTIALAQFLLPFIIG
                     FVAANSISFKVIFIVTAILIVVDAFLIAVLPFPPANNVIDNKGKTVKSEKMKFTPTSI
                     ALVCIGFTCTSTFVLWLNCNQELGKLYGMADPTKIQSFYSMGVICAVLITSLLIKKYI
                     KPIRILVIYPIIALLMLLVVYFVQTPTICMLGGFVIGYSAAGGVLQLTTSTANEMFPT
                     NKGKITSIVMIASSIANYVILNIAGIITKSGGVNGPKYVVLFNVAITFVGILLALFVN
                     MRYEKEKVYDYDV"
     gene            5592..7457
                     /locus_tag="PC414_002898"
     CDS             5592..7457
                     /locus_tag="PC414_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HDF2465280.1"
                     /translation="MENVFKRLQEFNGYDGYKESFEMNYLCIYESIPLREQVELANNL
                     VDEILNMYKSESNEIYLLEGSNSKSLICYFEIFMKKINTLVKEMIIDEKWLYKLTKEL
                     IYKSKKVEYVKLGLVLSEKYLNVENLREVVDTFSKSGEYVFYLSNTIKKLEFYNTYLF
                     NLSKKATGSIKVFAIVNMENLDSKINSYLIEDGYKDTKYERLLMNYIISIVDLNEYLE
                     KRDLDKEKINNLARLICNYLLSVEFKYIGNKLELVNRFLPTVVNYGTNFESLYSIFLI
                     AINVLKDENIECNKIEFEKEINGILLSEKWKNIYFEALRDASGKTEDIIKMSEIYDVN
                     LSFDDLLPYLNRDIRDFEVYWHISKKGTTSSRLKLLNFFEETFKIDDLIGKMKDIEKD
                     KLTQEYYDDMLFFIVLKGSKSLYPEGKNISLKGIFGNINEVRKESINILKRYREKLSL
                     EELKIVKEAYEKEKNVILKDELRRVLYESNNLKKEFVNIEKIKVDEHGKDIYLTSIAV
                     AGSRFRNREYLEKELEKSKIYYLTREKDNLYDEKAIKIVGETGYVIGYVPRKENYILS
                     NLLDGGKLLYCRVTEYNLYEDCIYANVYLSYKDVIETVENSLKMVLDKSRIKLIN"
     gene            complement(7635..8999)
                     /locus_tag="PC414_002899"
     CDS             complement(7635..8999)
                     /locus_tag="PC414_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2465281.1"
                     /translation="MSQLLIKNAYLITMNADREVYENGSILIENNIIKEVGKFDINIV
                     DKDAEIYDAKGKILMPGLVNTHVHLSQQLGRGLADDVVLLTWLRERIWPYESSFNYED
                     SLISSTACCVELIKSGVTTFLESGGQYVDAMVEAVDKTGLRACLAKSVMDTGDGLPEA
                     WQKTTDEEINTQLDLFHKFNNTSNERIKIWFALRTIFNTTEELLVRTKELADKYNTGI
                     HMHIAEIADEISFVKQNNGVGTVEYLDKLGVLGPNLLAAHTVWLTHKEIDLFRLHDVK
                     VSHDPGSAMKVVLGFASIPEMLEKGIPVSIGTDGAPSNNRMDLMRDMYLTSLIHKGRT
                     LNPTVVPAEEVLEMATINGAKCALLEKEIGSLEVGKKADMIILNPDTIHCLPMHNPIG
                     NIVYSMTSENVDSTICDGKWLMKERKVLVVDESELLNKVKKQASKIKDKAEVKIPSKF
                     KIIK"
     gene            complement(9178..10521)
                     /locus_tag="PC414_002900"
     CDS             complement(9178..10521)
                     /locus_tag="PC414_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDF2465282.1"
                     /translation="MVPNTNNVQPGGNNSSLSFLDKMFKFSERGSSLKTEVIGGVTTF
                     LTMAYIVFVNPAILSEAGMDKAALITVTILATAIGTLIFAILGNAPFALAPGMGLNAF
                     FTYSLVIGAGIPWQTGLGVVFLSGCVFFTLAVTGLRKTIADAIPRELAVASSAGIGLF
                     LAFVGLKNMGVIIANPDTIVSLADFTPTVLIALFALILMAILEYKKVKGGILISIVVA
                     TIISVIFGFVELPTAIISTPPSIAPVAFKLDIVGALKFSLIGPIFSFMFIDMFDSLAF
                     LITCCKQMGLEDENGEIKDLGRMLYADVSSTLIGSFLGTSTVTTFGESASGIAAGART
                     GLASIVTAALFLATLIFTPLLGIVPTYAAAPALVMVGVFMFESIKQIDFADSKIAVPA
                     FVTVLLMPLTYSISIGLCFGFISYIIMHVVAKETDKISITLWIIGLLAVVNLVLS"
     regulatory      complement(10594..10695)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            11024..11677
                     /locus_tag="PC414_002901"
     CDS             11024..11677
                     /locus_tag="PC414_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDF2465283.1"
                     /translation="MTKIIKVQKPKFTGELEIIENLEDILEDIFNDEKIFNKIIENEV
                     ISDLDSVRVSFESCIFRNVSFENCNLKKIDLLDVVFENCNLSNTFFDGGSIYRVEFKN
                     SKLIGVRFDDCILKNVLFKDVLGKYSNFSFSKFKIVSILDSNFENSVFQEVKGDNLVF
                     EYTNLKKANFNGTKLSGIDFTTNNIEGIEIGIDDIRGAIFDVSQAIDLTKLMGIIIK"
     gene            11818..13089
                     /gene="brnQ"
                     /locus_tag="PC414_002902"
     CDS             11818..13089
                     /gene="brnQ"
                     /locus_tag="PC414_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDF2465284.1"
                     /translation="MRDIFIVGFALFSMFFGAGNLMFPPFLGMESGKDWLIPLIGFVV
                     ADAGIAMLVIIATARCKGSMDDVLRRAGKGLARVMSVAALACLALLVIPRTCATTYEM
                     GIMPIFGDHGPVAKAVFSIIFFGLTLAFTIRSSKVIDIIGKYLTPALLLVLFVLIVKG
                     IISPAGPMSPEHMIDKNLFGEGISQGYQTMDALGAASMATIMLMSIIAKGYTSDKDQI
                     GMTIKAGFVACVFLAVVYGGLTYLGATVSTLYDTSIPQASLLVEITSILLYGQVGKVI
                     LGVIVALACLTTSTGLTASISTYFEGISKASYKQLVIGICVASMLISNLGVDSIIAIS
                     VPILQTIYPVLLAIVVMELAGKHIKNDNAFKGAAYVTLVISLLSAINGMTGAVPFIQS
                     LPLAGLGFNWVIPAILGGIVGNFIKSNKQVA"
     gene            13291..14478
                     /locus_tag="PC414_002903"
     CDS             13291..14478
                     /locus_tag="PC414_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HDF2465285.1"
                     /translation="MLRKITAFMLSICLIIGLTGCSLLVGKKDKEKIIDDFKVAVVTQ
                     PLSENKVQYNMVEEMAKEYEEENKIDKDKDGQTKVKQTIKHVVLPENFTSNIDSAINK
                     IVKLADDKEVQAIVVSTDQAGLLPALQKVKEKRPEIITISAPMGDDKNQLSQFVDVNL
                     GVSAEERGKVLAERSKEMGAKAFIHYASTDDLKDVNIAKRLEMIKETCKNIGLPFVQV
                     NTPNINTEEDKNKVKQFLNEDIEKQVKKYGKDINVFGVNEYMDEVILTKALELKYIVA
                     EQSNPSPIQTYPSVMGLKISEKDAQNYDKINDMISEKAKAFGMSNRLGGYPMPMDAFL
                     PSVAIYLATEMVKQDLTQEDVCDPDYLEAFTELRFGIGSEFTPLTEVLYNYQSVILSQ
                     LIY"
     gene            complement(14708..16732)
                     /locus_tag="PC414_002904"
     CDS             complement(14708..16732)
                     /locus_tag="PC414_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2465286.1"
                     /translation="MDKKKLILITYDKLNSDHYKEELTNFFGDEIIIETQNILDGIKE
                     NLEGDVVLSLSPLTSNFLIKHFKEDIEIIHGTKALSKLGYEKMMKLPPGTKSLLMTTN
                     KTSAFEMATYLYKIGINHIDFVPTYPDCDEIYDLDTAITPGQIRFIPKYIKNIVDLGW
                     RKISLDTYMSLLVVLKLKNEKFIEKLYKLSKETLSHDFLNTSLDNISKLKTILYMTID
                     EIGDGLIFFNTFNKVTFVNKSLLNMLELDEKLIKSPSLMEYMPKSFLDKITKNLNIDN
                     MIIYIDEIDKKFILSKKPFYLYKNIEGCLITLKDVNNIEILEQKIRSDSVKRGYVAKY
                     KFNNIIGNSSIIKDCIKKAKKMALTDNPILITGETGTGKEAFTQSIHNHSNRKNKPFV
                     AINCASLPSELLESELFGYEDGSFTGAKKGGKKGLFELAHTGTIFLDEIGDMPHDLQV
                     KLLRVLQEKEIRKIGGTSIIPIDVRILAATNKDLEKLIIENKFRMDLFYRISMFTLDL
                     PPLRKRLEDIPLLLESFLKELPYKNIKLDKSLLEALNSYTWMGNIRELRNCVEYMAYM
                     GSNYLTINDLPQNISSKLNNNHMSSNMSIFNDLNQYDKNICISILKSLHMKPMGRTKL
                     MKFMEYNVTEYEVRNMLEYLTRNGYLISSKGRKGSSITEKGIKIIENNII"
BASE COUNT         6155 a         2084 c         2796 g         5867 t
ORIGIN      
        1 aaagtctcgt gatacaataa atttataaaa tctagataaa tagaaatcaa aaaatacttt
       61 tatttttaag gtaaaataat actactagta ggataaataa aaatataaaa tcaaaaatac
      121 tattaattat aaagaaaata aagggggcaa atttaagcta tgaataatca aaaaaatctt
      181 aatattccaa ttacaattag ttcttataca ttgggaacag aagtttcatt ccgtgataga
      241 gttcgtattg caaaagaagc tgggtttgat ggtattggat tgcgtgctga aaattatatt
      301 gatgcaaaaa atgcaggtgt tacagatgaa gaaatgattg caattcttaa tgaatatgat
      361 atgaaagtta cagaagtaga atatataact caatggggaa gagaagaaga tagaacggaa
      421 gctcaaagag aaaaagagca aaatgtttat catatggctc acttatttaa tgttaaacat
      481 ataaattgtg gattattaga gatgatacca gaagaacaaa ttataactgc attgggagaa
      541 ttatgtgaca gagcaggtga acttattata ggtcttgagt ttatgccata tagtggtgta
      601 ccagatttgg ctactgcatg gcgtgttgtg aaaggatgta atcgtgaaaa tgcaatgctt
      661 atattagata cttggcactg ggctagagca aagcagacag cagaggacct tgcacctgtt
      721 ccagcagata agatagtatc aattcaagtg tgtgatgttt tagaaactcc ttatgaaaaa
      781 cttagggatg aatcactaca tgaccgtttg gctccaggtg aaggatatgg aaacacagag
      841 gaatttgtga aaataataaa agaacacaat atatcaccaa gagtcatagg ggcagaagta
      901 atatctgatt ctctaatatc taaagggtta tccactgctg ctgaaactgt atttaatgca
      961 actaaaaaag ttttggataa agcatggtct gaagtatctc caaaataaac ttatataata
     1021 taaaaatata cagagtatta taatataaaa cttgaaagga aggaaaacct tatgaagatt
     1081 agttctatgt ttacacctat aagaattggt tcaatgacag ttccaaatcg ttttgtagtt
     1141 ccccctatgg gaaacaattt tgcaaacaca gatggtactt taagtgaaac ttctaaggct
     1201 tattatcttg agcgtgcttt aggtggtttt ggattaataa ctatagaatc tagtgttgta
     1261 gacaaaaaag caaaaggagg tcctcgtaag ccttgtttat atgatgatag cacaatagat
     1321 agttttaaaa atgtaataga tgcttgtcat gatgcaggtt cgaaggtatc tatacaatta
     1381 caacatgctg gttctgaagg aaatgaaaaa gtagcaggac atccattaaa agctgcttct
     1441 gcaatacctg catcaaatgg tcgtaataca ccactagcaa taacaacaga agaaatatat
     1501 gaacttatag aatcttatgg agatgctgca ttacgtgcac aaaaagctgg tgcagatgct
     1561 gttgaagttc actgcgctca tggatatctg gttagtagtt ttatatcaca aagaacaaat
     1621 aagcgtgtag atgagtttgg tggatgtttt gaaaatagaa tgagattacc tcgtcttatc
     1681 attgaaaata tacgtaaaaa agtaggaaat tctctagcaa tattatgtcg tataaacagc
     1741 actgatgatg ttccaggagg aattgatgta cacgatagtt cagtaatagc agcatatcta
     1801 gaagattgtg gaatagatgg acttcatgtt tcacgtagta tacatataca tgatgaatat
     1861 atgtgggctc ctacgacttt acatgctgga ttcagtgcag aattagttac tgaaataaaa
     1921 aaagctgtta gtataccagt aataacagtt ggacgttata cagagccaca gtttgctgag
     1981 ttaatggtca gacaaggaag atgtgattta gtagcattcg gtagacaaag cttggctgac
     2041 ccagaaatgc ctaataaagc taaaaatgga aaactagacg aaatgatacc atgtattgca
     2101 tgcttacaag gatgtgtacc aaatatgttc caaggcaaac ctatagcctg tcttgcaaat
     2161 cctatattag gacatgaagc agagcttaag ccagctgaaa ttagcaaaga agttcttgtt
     2221 gttggtggag gtgttggagg tatgcttgca gcatgggtat gtgctaaaag aggacataat
     2281 gtaactttag ttgaaaaatc tgaagttctt ggtgggcaaa tgcgtttagc tgcatatcct
     2341 ccagggaaag gagatattac taacttagtt agaagttata tttcaaaatg taatcaatat
     2401 ggtgtaaaaa tatgtactaa tacagaagct acagtagaac ttataaagga gaaatcacct
     2461 gatgtagtta ttatagctac tggagcaact ccacttgtac ttcctatacc aggaatcaat
     2521 gattcaggat taattcatgc tgtagacctt cttgatggta aaaaatcttg tgggaaaaaa
     2581 gtacttgttg ttggaggtgg aatggttgga tgtgaagtgg cagccttcct tggagaacaa
     2641 gaacatgagg ttactgttat tgagcttaga gaagaagttg gtgcagatgt aatttcagaa
     2701 caccgtaaat tccttatgaa tgattttgaa aattataata tacataccat tactggtgca
     2761 aaagtttcta aattttatga tgatggtgtc gactatttac ttacagatgg cacagagcat
     2821 gatttaaaag gttttgattc agtagtactt gctatgggtt ctcgtaatta cgacccatta
     2881 agtgaagtta taaaagaagt tgtaaaagaa acttatattg ttggtgatgc agtaaaagct
     2941 cgtagggcat tagatgcaac aaaagaagct tttgaagtag cattaaatat ataaataata
     3001 aataaatttt tctttgtaaa taactgaata ggttatttgc aaagaaaatg ataatattga
     3061 gaggagatta tagttatgga aatttcaggt agaacaggat tatttgcatt aataggtaca
     3121 ccggttggtc actctaaatc accagtgatg tataattata gttttaagaa attagattta
     3181 gattatagat atttagcctt tgatatcaca gtagataagg taaaagaggc acttttagca
     3241 ataaaaactt ttaatataaa aggagccaat gttactatgc catgtaaatc tgctgttaca
     3301 gaatatatgg atgaactttc tccagcagct cgtataattg gagcatgtaa tacaattgta
     3361 aatgacaatg gaaagttagt tggtcatata acagatggag taggctatgt tcgtaattta
     3421 aaagaaaatg gggtagaagt taaaggtaaa aagattacaa ttatgggtgc tggtggtgca
     3481 gcaactgcta tacaagtaca atgtgcttta gatggagcaa gagaaatatc tatctttaat
     3541 cctaaggatg atttttataa aagagcagag caaacagttg agaatataaa aaaagatgta
     3601 ccagaatgtg ttgttaatct atatgattta gaagatacaa acaaacttta tgaggagata
     3661 caatcaagtg atatattgac aaatgcaact cttattggta tgaagcctta tgacaatgaa
     3721 acaaatataa aagatactag tgttttaaga aaagatttag tagtaacaga tgtcgtttat
     3781 aatcctaaga aaacaaaaat gattgaagat gctgaagcta atggatgtaa ggctattggt
     3841 ggattaggaa tgttattata ccaaggagca gaagctttta atctatatac tggattagaa
     3901 atgcctgtag aagaagttaa tgaattatgc tttaaataga taagagtaat atcttaaaca
     3961 atgaaaaaac atacgtattt caatattttt aataataaat tacaaattgt catagtgggt
     4021 tttgcctcat agtccagtta aatttgaagc tttaactgac tatactttaa atccattatg
     4081 gggatgagtt tattgtacac aaaaatgtta ataaattaac aagaatggga gaaaaattat
     4141 gaacaataaa aaatattatc ctactgccat agcgttgtat ttttcatatt ttttacttgg
     4201 tattggaatt tcaattttag gacaatacaa accagaattt tcatcaatgt ggggggctaa
     4261 aacattatca gatggaacat tggatgtaag tattgtttta gctgttattg ctgcactagg
     4321 attagggaga cttatatctt atccatttgc aggacctatt tcagataagt atggaagaaa
     4381 agtaagtggt cttataggaa actttctaca tgcaattttc tttgttggaa ttgtattttc
     4441 accaaatttt tatatagcat atgtatttgc aataattgga ggggcagcaa attcattctt
     4501 agatacctgt gtaactcctt cttgtatgga gatatttgct agtttaggaa caatagcaaa
     4561 tatgtttact aaatttacaa tagctttagc acaatttttg cttcctttta taattggatt
     4621 tgttgcagca aattcaatat catttaaagt tatttttata gtaacagcga ttcttattgt
     4681 agtagatgcc tttttgatag ctgtgttacc atttccacca gctaataatg ttattgataa
     4741 caaaggcaaa acagttaaat cagagaagat gaaatttaca ccaacaagta tagctcttgt
     4801 ttgtattgga tttacatgta catctacatt tgtattatgg ttaaattgta atcaggaatt
     4861 gggtaaattg tatgggatgg ctgaccctac aaaaattcaa tccttttact caatgggagt
     4921 aatatgtgca gtattaataa cttctttact tattaaaaaa tatattaaac caataagaat
     4981 attagttata tatcctataa tagctttact tatgctatta gttgtatatt ttgttcaaac
     5041 tccaactatt tgtatgttag ggggatttgt aataggttat tcagcagcag gaggtgttct
     5101 tcagttaaca acgtcaactg caaatgaaat gtttccaact aataaaggaa aaataacatc
     5161 tatagttatg attgcatcta gtatagcaaa ctatgttatt ttaaatatag caggtattat
     5221 tacaaaatca gggggagtta atggaccaaa atatgttgta ttgtttaatg tggcaataac
     5281 atttgttgga attttacttg cattgtttgt aaacatgaga tatgaaaaag aaaaagtgta
     5341 tgattatgat gtctaatatt agtataatat atgcatagag aagtctaaat ctaggataga
     5401 tttttattaa aagtaattaa aattaataag gatattattt acagttataa gatagggtat
     5461 ttacataatt aataataagg atattactag aatttcaatt tctagtaata tttttttgcg
     5521 tataaatgta aacttgatga aataagtagt ataatcaata taaattacat catgtaaagg
     5581 aggataattt ggtggagaat gtttttaagc gattgcagga atttaatggt tatgatggat
     5641 acaaagaatc atttgaaatg aattacctat gtatatatga aagtataccg ttaagagaac
     5701 aagtagagct tgcaaataat ctagtagatg aaattttaaa tatgtataaa agtgaatcta
     5761 atgaaattta tttattggag ggtagtaatt ctaagtcttt aatttgttat ttcgaaattt
     5821 ttatgaaaaa gataaatact ttggtaaaag aaatgattat tgatgagaaa tggttatata
     5881 aattaactaa agaattaata tataagagta aaaaagttga gtatgtaaaa ctagggttag
     5941 ttttaagtga gaaatatcta aatgtagaaa acttaagaga agttgtagat acattttcta
     6001 aatctggaga atacgtattt tatctgagta atactattaa aaaacttgaa ttttacaata
     6061 catatttatt taatttatct aaaaaggcta caggttcaat aaaagtattt gctattgtta
     6121 atatggaaaa tttagattct aaaataaatt cctatctaat tgaagatggt tataaggata
     6181 caaagtacga acgattattg atgaactata ttatttcaat tgtagatttg aatgaatatc
     6241 ttgaaaaacg agatttagat aaagaaaaga taaataattt agcacgtctt atatgcaatt
     6301 acttgctaag tgtagaattt aaatatatag gtaataaatt agaattagtg aatcgatttt
     6361 tacctactgt agtaaattat gggactaact ttgaaagttt atattcaatt ttcctaatag
     6421 ctataaacgt cttaaaagat gaaaatatag agtgcaataa gattgaattt gaaaaagaaa
     6481 taaatggtat cttgctcagt gaaaaatgga aaaatatata ttttgaagca ttaagagatg
     6541 ctagtggaaa aacagaagat attataaaaa tgtcagaaat ttatgatgta aacttatctt
     6601 ttgatgactt gttgccatat ctaaatagag atattagaga ttttgaggta tattggcata
     6661 tatctaaaaa gggaacgaca agttctagat taaaattatt aaattttttt gaagagacgt
     6721 ttaaaataga tgatttaatt ggaaaaatga aagatataga aaaagataaa ttgactcaag
     6781 aatattatga tgatatgtta ttttttattg tgcttaaagg aagtaaatca ctatatcctg
     6841 aaggaaagaa tatatcttta aaaggaatat ttggaaatat aaatgaagta agaaaagaat
     6901 ccataaatat cttaaaaaga tacagagaga aactatctct tgaagaatta aaaattgtta
     6961 aagaagcgta tgaaaaagaa aaaaatgtta tattgaagga tgaattaaga agagtattat
     7021 atgaaagtaa taatttaaaa aaagaatttg taaatattga aaaaataaaa gtagatgaac
     7081 atggtaagga tatctatttg acttcaatag ctgttgctgg ttcaaggttt agaaatagag
     7141 aatatcttga aaaagaattg gagaaaagta agatatacta tttaactaga gagaaagata
     7201 atctatatga tgaaaaagct attaaaatag ttggtgaaac tggatatgtg ataggctatg
     7261 tcccaagaaa agaaaactat attttgagca atttattaga tggaggaaaa ctgctgtatt
     7321 gtagagtaac tgaatataat ttatatgaag attgtatcta cgcaaatgtg tacttgagtt
     7381 ataaagatgt aattgaaaca gttgaaaact ctttaaagat ggttttagat aagagtagaa
     7441 taaaacttat aaactaagac gtttatatat tgcttagatg atgtaatcct aactgattgg
     7501 ttattttttc tggaattatg gtgtatttta tgagcaatgc taatgaatat aaaataaaaa
     7561 aacaattgtt ttgtatatat gatgtatata aatatattta tatattcata tttacaaaac
     7621 aattgaaatt tatgttattt tataatttta aatttagatg gaattttaac ttctgcttta
     7681 tcttttattt tagaagcttg ttttttaact ttatttaata attcagattc atctacaact
     7741 aatacttttc tttccttcat caaccattta ccatcacaaa tagtagagtc tacattttca
     7801 ctagtcatag aataaactat atttccaatt ggattatgca ttggtaaaca atgaattgta
     7861 tctggattta gaataatcat atcagctttt tttcctactt caagactacc aatttctttt
     7921 tctagtaaag cacattttgc tccatttata gtagccattt ctaagacttc ttcagctggt
     7981 acgacagttg ggtttaaagt tcttccttta tgtattagtg atgtaagata catgtccctc
     8041 ataaggtcca ttctattatt tgaaggagct ccatcagtac caattgaaac aggtattcct
     8101 ttttctaaca tttcaggaat tgatgcaaac cctagaacca ctttcatagc tgaacctggg
     8161 tcatgagaaa cttttacatc atgtaatcta aataaatcaa tttccttatg agtaagccaa
     8221 actgtatgag cagctaaaag atttggtcct aatacaccta gtttatcaag gtattcaact
     8281 gttccaactc cattgttttg ctttacaaat gaaatttcat cagctatttc agcaatatgc
     8341 atatgtatac ctgtattgta tttatcagct agttctttag ttctaacaag taattcttca
     8401 gttgtattaa aaatagttct taatgcaaac catattttaa ttctctcatt agatgtatta
     8461 ttaaacttat ggaataagtc taattgagtg ttgatttcct catcagtagt tttttgccat
     8521 gcctcaggaa gaccatctcc tgtatccata actgatttag ctaaacaggc tctaagtcca
     8581 gttttatcaa cagcttcaac catagcatct acatattgac caccagattc taaaaatgta
     8641 gtgacaccag attttatcaa ttcaacacaa catgcagtag atgaaatcaa ggaatcttca
     8701 taattgaagc tactttcgta tggccaaatt ctttctctaa gccaagttaa aagtacaaca
     8761 tcatctgcaa gaccacgtcc taattgttga gataagtgaa catgagtatt gactagccct
     8821 ggcattagta tttttccttt agcatcatat atctctgcat ctttatctac aatattaata
     8881 tcaaacttac caacctcttt tataatatta ttttcaataa gtatgcttcc attttcatat
     8941 acctctctat ctgcattcat agtaattaga tatgcatttt ttataagtaa ttgtgacata
     9001 aacaccactc cttttctata tatttttata tctatatatt atgataataa attttctata
     9061 caaagttaat agcaaaatca atttacatat aaagtgattt tgctatttat aaatttaaaa
     9121 taaataagat tttattctat ataatttaaa atattgacct agaactattt aaaaatatta
     9181 acttagaact aagtttacaa ctgcaagtaa acctataatc caaagtgtaa tactgatttt
     9241 atcagtttct ttagctacta catgcattat tatatatgaa ataaatccaa agcataaacc
     9301 aatacttata ctataagtta aaggcatcaa taaaactgta acaaaagcag gtacagctat
     9361 tttgctatca gcaaaatcaa tctgttttat gctttcaaac ataaacacac caaccattac
     9421 aagtgcagga gctgcagcgt aagtaggaac tataccaagt aatggtgtaa atataagtgt
     9481 agctaagaat aatgctgctg ttactattga agcaagtcct gttcttgcac ctgctgcaat
     9541 accagaagca gattctccaa atgttgtaac agtactagtt cctaagaatg agcctattaa
     9601 agtagaagaa acatctgcat ataacattct tcctaaatct tttatttcac cattctcatc
     9661 ttcaagaccc atttgtttac aacaagtaat aagaaatgct aatgaatcaa acatgtctat
     9721 aaacatgaaa gagaatatag gtccaattaa tgagaatttc aatgcaccta caatatctaa
     9781 tttaaaagca actggtgcta tactaggagg tgttgagatt attgctgttg gcaactccac
     9841 aaatccaaat ataacagata ttattgttgc aactactata cttatcaata ttccaccttt
     9901 aactttttta tattctagta ttgccattaa gataagagca aaaagggcta ttaaaactgt
     9961 tggtgtaaaa tctgcaagtg aaactatagt atctggatta gctattataa cacccatatt
    10021 ttttaaacca acaaaagcaa ggaaaagacc tatacctgct gaagatgcta ctgccaattc
    10081 tctaggaatt gcgtctgcaa ttgtctttcg aagacctgtt actgcgagtg taaagaagac
    10141 acatccagat agaaaaacta ccccaagacc tgtttgccat gggattcctg cacctataac
    10201 caaagagtat gtgaaaaatg catttaatcc cataccagga gctagagcaa atggtgcatt
    10261 tcctaaaatc gcaaatataa gtgtaccaat ggctgtagct agtatggtaa ctgttataag
    10321 ggcggcttta tccattccag cttcagataa tattgctgga tttacgaaaa ctatgtatgc
    10381 cattgttaag aatgtagtaa ctccacctat tacttctgtt ttaaggctag agccacgttc
    10441 gctaaattta aacattttgt ctaaaaagct taatgatgaa ttatttccac ctggttgtac
    10501 attgttagtg tttggtacca tttttacctc caaaaaactt taagatataa acaaaaaaat
    10561 atatattcat atagatagaa tagaacatat attcagagtt gacattctat tcatagaaaa
    10621 cccatttaag gtgagtttgt agaaactatt agaccgtatt actaatatta tatgaacatt
    10681 ttctatttgt ttttctgttg tattatatcg taatattgta ttatttacaa taaatataat
    10741 aaaattattc gtttttttgt cgattttata aaaaattaag gttatttatt gaaaaaaatt
    10801 actttttcca ataattgaat atgtatgagt ataatgtcaa ttattctaac ttgttttata
    10861 ctattaatgg aagtttatta taaatgagtt tgtgatataa ttataaaagt actgaataca
    10921 tggatatttc gtaaaaactt tttaatatat caaatatgta tattaagtta atatattaaa
    10981 aaaaataata tcaaaaataa tataatttat agtggagggg aaaatgacta aaattattaa
    11041 agtgcaaaaa cctaaattta caggagaatt agaaattatt gaaaatcttg aagatatact
    11101 tgaagatata tttaatgatg aaaaaatatt taataaaatt atagaaaatg aagttataag
    11161 tgatttagat tctgtaagag ttagctttga atcttgtata tttagaaatg tgtcttttga
    11221 aaattgtaat ttaaaaaaga ttgatttact tgatgtggta tttgaaaact gcaatttatc
    11281 aaatacattt tttgatggag gaagtatata cagagttgaa tttaaaaata gtaaattgat
    11341 aggagttaga tttgatgatt gtatacttaa aaacgttttg ttcaaggatg ttttaggtaa
    11401 gtattctaac ttttcattct ctaaatttaa gatagtttct atcttagatt caaattttga
    11461 aaattctgta tttcaagaag taaaaggtga caatctagta tttgagtata caaatttaaa
    11521 aaaagcaaac tttaatggaa ctaaactttc tggcatagat tttactacta ataatattga
    11581 gggaattgag attggtatag atgatattag aggagcaatt tttgatgtgt cacaagcaat
    11641 tgatttaacg aaattgatgg gaattatcat aaaatgaaaa aaatattcat aaaataaatt
    11701 gggaaaacgt taacaatgtg tgaattttta ccagattgac atttttctga aaataaagta
    11761 agattatctt aatattcaaa atattttcaa agggggtaat ttagtgtgag taagcagatg
    11821 agagatattt ttatagttgg ttttgcttta ttttctatgt tctttggtgc aggaaatctt
    11881 atgtttcctc catttttagg gatggaatcc ggcaaagact ggttaattcc tcttattgga
    11941 tttgttgttg cagatgctgg tatagcaatg cttgttatta tagcaacagc aagatgtaag
    12001 ggaagcatgg atgatgtact taggagagct ggtaaagggt tagcaagagt aatgagtgta
    12061 gctgctctag catgtttagc acttcttgta ataccaagaa cttgcgcaac aacatatgaa
    12121 atgggtatta tgcctatatt tggagaccat ggtccagtag ctaaagctgt attttcaata
    12181 atcttctttg ggctaacttt agcatttact attaggtctt ctaaggttat agacataata
    12241 ggtaagtatt taactccagc gttattatta gtattatttg ttttaatagt aaagggtata
    12301 atttctccag caggacctat gagtcctgag catatgatag ataaaaatct atttggtgaa
    12361 ggtatatcac aaggttatca aacaatggac gctctaggtg cagcatctat ggcaactata
    12421 atgttgatgt ctataatagc taaaggatat acatcagata aagaccaaat aggaatgact
    12481 attaaagctg gttttgtagc ctgtgtattt ctagcagtag tatatggtgg tcttacttat
    12541 ttaggtgcaa cagtttcaac tttatatgat actagtatac ctcaagcatc tttattagtt
    12601 gaaataacta gtatattgtt atatggtcaa gtaggaaagg taattttagg tgtaattgtg
    12661 gcacttgcct gtctgactac ttctacaggt ttaacagcat caatatcaac ttattttgaa
    12721 ggaataagta aggcgagtta taaacaactg gttataggta tatgtgtagc tagtatgctt
    12781 atatcaaatt taggtgttga ttctataata gccatatcag ttccaatcct acaaactata
    12841 tatcctgttt tattagcaat agttgttatg gagttggctg gtaaacatat aaagaatgat
    12901 aatgctttta aaggtgcagc atatgttaca ttagtgatta gtttattatc agctataaat
    12961 ggaatgacag gagctgttcc atttatacaa agcttgccac ttgcaggttt gggctttaac
    13021 tgggtaatac cagctatatt gggtggaata gttggtaact ttataaaaag caacaaacaa
    13081 gtagcataat aatttgtaat taatttaatt ttataatata ttaattttta gtactatggg
    13141 taggtttaat ctaaacctaa tctacccata gtacttttta gattggatta taatagcatg
    13201 tatataattt tttataatat aatttttctt tgaagtatga taaaattagt aatagtgata
    13261 agaatgtaca aatttaggag gttttaatta atgcttagaa aaataacagc atttatgcta
    13321 tccatatgtt taattattgg acttacaggt tgtagtttac ttgtaggcaa gaaagataaa
    13381 gagaaaatta tagatgattt taaagttgca gtggtaactc aaccattatc tgaaaataaa
    13441 gtacaataca atatggtgga agagatggca aaagaatatg aggaagagaa taaaattgat
    13501 aaagacaaag atggtcaaac taaagttaaa caaactataa agcatgttgt attgccagag
    13561 aattttactt ctaatataga cagtgctatt aataagatag taaagttggc tgacgataaa
    13621 gaagttcagg ctattgtagt ttcaactgac caagcagggc ttttacctgc tttacaaaag
    13681 gtaaaggaaa agcgtccaga aataataact atttctgccc ctatgggaga tgataaaaac
    13741 caattaagcc aatttgttga tgtgaatcta ggggttagtg cagaggaaag aggaaaagta
    13801 ttagctgaac gttcaaaaga aatgggagca aaagctttta ttcattatgc ctctacagat
    13861 gatttgaaag atgttaatat agccaaaaga ttagaaatga ttaaagagac ttgcaaaaat
    13921 ataggattgc catttgtaca agttaatact ccaaatataa atactgaaga agacaaaaac
    13981 aaagttaagc agtttttaaa tgaagatatt gaaaaacaag ttaaaaaata tggaaaagat
    14041 ataaatgtat ttggtgtaaa tgagtatatg gatgaagtta tattgactaa ggctttagaa
    14101 ttaaagtata tagttgcaga gcaaagcaat ccatcaccaa tacaaacata tcctagtgtt
    14161 atgggtctaa aaatatctga aaaagatgct caaaattatg ataaaataaa cgacatgata
    14221 agtgaaaagg ctaaggcttt tggcatgtca aatagacttg gtggatatcc tatgccaatg
    14281 gatgcttttc taccatcagt agcaatttat cttgctactg aaatggtgaa acaagattta
    14341 acacaagaag atgtatgtga cccagattat ttagaggcgt ttacagaatt aagatttgga
    14401 atcggttcag aatttactcc tttaacagag gtactttata attatcaatc agtaatttta
    14461 agccaattaa tttattaata ttaaggacgt gtctcaaaat gaacttttta gtttatgagg
    14521 cactcttttt ctgttaaatc aaatatattt ttatcttttt aacaattaaa aagaagctta
    14581 tccatatgtt gagatagctt cgtttttaat ataaataagt tttattattt tataaaaaat
    14641 ttatagaaaa ataaaaaatt ataattatat ataaattttg ataaagtaaa gatgaattac
    14701 tataggttta aattatatta ttttctatta tttttatgcc tttctcagtt attgatgagc
    14761 cttttcttcc cttactagaa attaagtatc cattgcgtgt caaatattct agcatatttc
    14821 ttacttcgta ttctgtgaca ttatattcca taaatttcat taactttgtc ctccccattg
    14881 gtttcatgtg caatgatttt aggatagaaa tgcatatgtt tttgtcatat tggtttaaat
    14941 cattgaatat ggacatatta cttgacatat gattattatt taattttgaa gatatgtttt
    15001 gaggtaaatc atttattgta agataattgg aacccatgta agccatatat tcaacacaat
    15061 ttctcagttc tctaatattc cccatccaag tgtatgaatt taaagcttct aaaagactct
    15121 tatcgagttt tatgttttta tagggaagtt cttttaaaaa tgattctaaa agaagaggta
    15181 tatcttctaa tctctttcta agaggaggta aatctaaagt gaacatactt atcctataaa
    15241 ataaatccat cctaaattta ttttctataa ttaatttctc taaatcttta tttgtggcag
    15301 ctaatattct cacatcaatt ggaataatac ttgtaccacc aatttttcta atttcttttt
    15361 cttgtaagac tcttaaaagt ttaacctgta aatcatgtgg catatctcct atctcatcta
    15421 aaaatatagt gccagtatga gctaattcga ataatccttt ttttccacct ttttttgctc
    15481 cagtaaaaga accatcttca tatccaaata gttcactttc aagtaattca cttggtaatg
    15541 aagcacaatt tattgctaca aaaggtttat tttttctatt tgaatgattg tgtattgact
    15601 gagtaaaggc ttctttacct gttcctgttt cacctgttat taatatagga ttatctgtaa
    15661 gagccatttt ttttgccttt ttaatacaat cttttataat agaactattt ccaataatat
    15721 tattaaattt atatttagct acataacctc ttttgacaga atcacttctt attttttgtt
    15781 ctaatatttc tatattgttt acatctttta atgtaattaa gcaaccctct atatttttat
    15841 aaagataaaa tggtttttta gaaagtatga attttttatc tatttcgtct atgtatataa
    15901 tcatattatc tatatttaag ttctttgtaa ttttatctaa gaaactcttt ggcatatatt
    15961 ccattaagga aggtgatttt ataagttttt catctagttc caacatatta agtaaagact
    16021 tatttacaaa agtaacctta ttaaaggtat tgaaaaatat tagaccatca cctatttcat
    16081 caatggtcat gtaaagtata gtttttaatt tagatatatt atctaatgat gtatttaaga
    16141 aatcatgact tagtgtctcc ttggacaatt tgtataattt ttctataaat ttctcatttt
    16201 ttagttttaa tactactaat aatgacatat atgtatctaa tgagattttt ctccaaccta
    16261 aatcaacaat attttttatg tattttggta taaatctaat ctgacctggt gttatagctg
    16321 tatctaaatc atatatttca tcacaatctg gatatgtcgg tacaaaatct atatgattta
    16381 taccaatttt atatagataa gtagccatct caaatgctga agttttattg gttgtcataa
    16441 gtaacgattt tgtacctgga ggcagtttca tcattttttc atatccaagt tttgaaagtg
    16501 ctttcgttcc atgaataatt tcaatatctt ccttaaaatg ttttattaaa aaattactag
    16561 ttaaaggtga tagagaaagc actacatctc cctctaaatt ttcttttatt ccatcaagta
    16621 tattttgtgt ttctatgatt atttcgtctc caaaaaagtt tgttaattct tctttgtagt
    16681 ggtcactatt caatttatca tatgttataa ggattaactt ctttttatcc attttatttc
    16741 tcctaacaaa atagtattta tttttaaagt atttaaagta taaggcaagt tatattatac
    16801 aacaaatagt agtataaatt tatatatttg attaaataat aggttttttt aggattttat
    16861 cctataaaaa cctattttga aattattttt gcaagataag ga
//
LOCUS       DAMJVK010000089        16831 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.091, whole genome shotgun sequence.
ACCESSION   DAMJVK010000089 DAMJVK010000000
VERSION     DAMJVK010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16831
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.091"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            293..751
                     /locus_tag="PC414_002905"
     CDS             293..751
                     /locus_tag="PC414_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2465287.1"
                     /translation="MEFINFVSDKVIDLKMDCKTKEDFFKEIHNKVFDLGFVKEEFGE
                     KILARENVFPTGLNLGDYGVAIPHTDAEYIKEQFISVCTFNEPVVFSSMEDQDEKVPV
                     NLAFVLGLNQPHSQLSVLTELMGIMQNKELVDKLMSSTDKEEVLKTLKSL"
     gene            812..1087
                     /locus_tag="PC414_002906"
     CDS             812..1087
                     /locus_tag="PC414_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2465288.1"
                     /translation="MKKILVACGAGIATSTVVCDKVERLVKENNIQAEVIQCKIAECA
                     TKQEGADLIVSTTILPTTYNIPAIKATAYITGVNTAALDKKILDALK"
     gene            1146..2420
                     /locus_tag="PC414_002907"
     CDS             1146..2420
                     /locus_tag="PC414_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012951111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDF2465289.1"
                     /translation="MDKLLAVVQYILNMGPTVILPITIVVLGMIFRIKFTKALKSGLT
                     IGIGFVGINLVVNLLTSTLGPAAQGMVERFGLNLTVIDVGWPTTASATWASPVAPILI
                     PVCLLVNLLLLYFNKTKILNVDIWNYWHFIVAGAVGYIVTGSYVWAIFCAILMELLVL
                     FIAEKTAKNVQEFYDLEGIALPTGSTASFAPLGYLVGKFVEKIPGINKIQADPDSIQK
                     RFGIFGEPMMMGIILGIILGALAGYDVAGIFNVGISMGAVMLLMPRMVRILMEGLIPI
                     SESIRDFLQAKYGDRELYIGLDAAVATGHPAVISTALILVPITLFLAVILPGNKVLPF
                     GDLATIPFYMAFIVCFRKGNIVQSVITGTIVIAISLYMATNFADVHTQLLQQANMMPE
                     GTTMVTSIDTGGNLLKWGILKLSQLVSSFGIF"
     gene            2550..3602
                     /locus_tag="PC414_002908"
     CDS             2550..3602
                     /locus_tag="PC414_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDF2465290.1"
                     /translation="MKAAVLHGTNDMRFEDIEIKPCESDEVKIKVMAAGICGSDPPRV
                     LKHWKYPVPAIPGHEFSGVIAEVGKDVKNVKVGDRVVAIPFIPCNECEYCKRGLFSLC
                     DDHGMLGAKSFGAFAEYVNIKATNVLPIGDMNFEDAAMIEPLAVAMHGVLNIGVQVGD
                     TVAVMGSGTMGQLVIQGLKIAGAGTIIAVDISDNKLRESKELGADIIINAKDINPVEK
                     IKELTGGKGVDIALECAGSKITQEQCLLITKKKSKIGFLGIAYSDITLSEEAFENIFR
                     KELELKGFWNSYSAPFPGQEWTKGINLVNEGKIKLKEMVSHRFSLEDTYKAFEMIRDR
                     KEEFNKILILPQGVEK"
     gene            3602..4633
                     /locus_tag="PC414_002909"
     CDS             3602..4633
                     /locus_tag="PC414_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HDF2465291.1"
                     /translation="MKAVVKTKPGYDNVEVLEVEEPKATGDKVKIKVEYSGICGSDIH
                     SFKGEYANIKAPVTLGHEFSGIVVEVGEDVKNIKVGDRVTSETTFATCEKCIYCETKD
                     YNLCPTRKGIGTQVNGSFAEYVLSREESIHVLPENVSLLSAALTEPLACCVHAALEKT
                     TIESSDTVLIIGPGPIGLLLAQVVKSQGATVIMSGVTKDKERLEIAKNLGVDRTVNVM
                     EESLADVVNEMTDGNGVNKGFDCSGFMPAVNEALRLLRKKGTFVQVGIFAKKLNEMDQ
                     EAIIQRELQYIGSRSQKPSSWITALDLMANGKVNTEALVTRTVGLDEFKEGITALMNG
                     EEIKVAVKS"
     gene            4725..5537
                     /locus_tag="PC414_002910"
     CDS             4725..5537
                     /locus_tag="PC414_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDF2465292.1"
                     /translation="MSTLRDHSNNIRKNIIKMVAEAKSGHPGGSLSIADMLTVLYFDK
                     MNVCAENPKMADRDRFVLSKGHAAPALYATLAEKGFFPEEELITLRKFGSKLQGHPDM
                     KKVAGIDMSTGSLGQGLSAANGMALAGKLDNKDYTVYTILGDGEIQEGQIWEAAMTAS
                     HYKLDNLIAFVDLNGLQIDGSNEEVMNVSPVDDKFKSFGWNVIVINGHSFEEIGNAVD
                     EAKKVTGKPTVIIAKTVKGKGVSFMENNAAWHGTAPNAEQAEQALKDLEGGL"
     gene            5540..6460
                     /locus_tag="PC414_002911"
     CDS             5540..6460
                     /locus_tag="PC414_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HDF2465293.1"
                     /translation="MGIATREAYGQVLKELAENKDIVVLDADLGKATKSISFKEVAPD
                     RFFDMGIAEGDMIGTAAGLATCGKIPFASTFAIFAAGRGYEQIRNSVAYPNLNVKIAA
                     THAGVTVGEDGGSHQAIEDISLMRGIPNMVVLNPADALEARQAILTSIDYNGPVYIRL
                     GRAATPDVNSENYKFEIGKGTVLREGSDITVIATGIMVAKALEAAEELAKEGINVEVV
                     NISTIKPLDEALIKETAKKTGKVVTAEEHSIIGGLGSAVCEALAETKDVVVRRIGVKD
                     VFGQSGTPADLLKHYGLTTEDIVKNIKELL"
     gene            6635..7081
                     /gene="rpiB"
                     /locus_tag="PC414_002912"
     CDS             6635..7081
                     /gene="rpiB"
                     /locus_tag="PC414_002912"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HDF2465294.1"
                     /translation="MKISIGCDHGGYELKEYIKENLINKGIEVEDVGTNSKESVDFPI
                     YAKKVSQNVQSKKSDLGILCCGTGIGMSIAANKFKGIRAAVVSDCFSAKATREHNNTN
                     VLCLGERVIGKGLAMRIVEEWLNSSYNGERHARRLQMIDDIEKENL"
     gene            7111..7776
                     /locus_tag="PC414_002913"
     CDS             7111..7776
                     /locus_tag="PC414_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDF2465295.1"
                     /translation="MSIICASIMCADQMKLKEELEALEEAGVKLLHCDVMDGIFVKNL
                     AMGPELLKSISENTTIPLDIHLATETPDKYIDMMSYIKPKYISFHVESSTNVKADIQK
                     LRNYGIGPVLAISPQTSVDKIEEYISLVEGILVMTVNPGFAGQKFNLSVLDKLDKLTE
                     ILKDYDNPPFIEVDGNINKDTIKLMNGKKVDIYVVGTSALFNDKPPISYKDKIEELKE
                     SIK"
     gene            7886..9388
                     /locus_tag="PC414_002914"
     CDS             7886..9388
                     /locus_tag="PC414_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HDF2465296.1"
                     /translation="MGDLYKLQNGTDIRGIAYENDSKEVNLTVEEVKKIAKAFHIWLK
                     EKTKKDKVTVAIGTDSRITGSQFRSTVIETLTNDDCNVIDCEIATTPAMFMTTIMDGY
                     NSDGAIMITASHLPYYYNGLKFFTESGGLEKTDIKEMLDIAVKNDSVDYEEVNKKGEV
                     IVKNLIEDYSNLLIDKIRKGVNSSKNYEKPFSGLKILVDAGNGAGGFFAEKVLHILGA
                     DTTGSQFLNPDGMFPNHIPNPENKEAMESICKAVLDNKSDLGIIFDTDVDRAAIVGKN
                     GKPINKNALIAVISSIVLEEHPKTAIVTDSITSEGLAKFINELQGRHHRFKRGYKNVI
                     NEAIRLNSEGEECHLAIETSGHAALKENYFLDDGAYLIAKILIKVAKLSLEGKTIEEL
                     IENLEEAQEEKEVRIGINKEDFRPYAEDILKDLDSHVKSIDGWSVAPNNFEGIRVNCD
                     ESNGDGWFLLRISLHEPLLALNIESNKIGGAEQIYSKLKLFLEKYDLKGL"
     gene            complement(9700..11292)
                     /locus_tag="PC414_002915"
     CDS             complement(9700..11292)
                     /locus_tag="PC414_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2465297.1"
                     /translation="MVTKLKNLIINDSKAKFILLLLLFLLVSILFEAVYDLNNNFSYN
                     INYYYKEFALPYFYFFGTPVHLSKVELEFVCIGIILSSFLLTILCFTLYKKNKQTKVK
                     FLENVISLFSKVKIELTLILTLILLTLRFHAFTSNKMFYNTNLIIYNFIIISIVYIIY
                     ENIKYNNYNLKDMSITYVFFSNLLNMYNKKTINKKLAIVFLISIVVQAIIQYLVLIFF
                     MYIYYAEAIAIVILFTLVNTVFHFYIYNFLSVKFNYINNISKNIKNIENGNLKYKLEV
                     IGDDEISTLAKSINNITGGLETAVDANIKNERMKAELITNVSHDLKTPLTSILSYVDM
                     LKNNDFDRETINDYINILDKKSQRLKLLIDDIFEASKLSSGDVEFNITKTDIRELLIQ
                     SIVEFEDKIQNSSLDFIIETPEYAVFTMIDGKKTWRVFENLICNILKYSMPNTRVYID
                     MFIKEENIILTFKNISNDKLNLKPEELIERFRRGDISRKTDGSGLGLSIAQNIIELEN
                     AHMEIIIDADLFKVMLTFRRVN"
     gene            complement(11261..11977)
                     /locus_tag="PC414_002916"
     CDS             complement(11261..11977)
                     /locus_tag="PC414_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2465298.1"
                     /translation="MNQNILVVDDDREIVDAIEFYLRPDNFNVLKAYDGLEALDILIE
                     NDIKLIIMDVMMPNLDGLKTTLKIREKRNIPIILLSAKSEDMDKIIGLNMGADDYITK
                     PFNPLELTARVKSQLRRYINLGDFSNTNNSNDTNYILKSGGLELNTDTKIVSLDGEEI
                     KMTPLEYKILTLLMSRKGKIFSSHEIYENVWNEDAYSCERTIAVHIRRIREKIEINPK
                     EPKYLKVVWGIGYKIEKLNY"
     gene            complement(12517..13317)
                     /locus_tag="PC414_002917"
     CDS             complement(12517..13317)
                     /locus_tag="PC414_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HDF2465299.1"
                     /translation="MTFKNKKFTINKYKILSIILVLCLILTAFSSYVNYSHKKFKDYV
                     VFYVQHQDDEVLWAGSAIVNAIKERGKNHVFVVLVSTGCGISVFDKYKKYENLTNIQK
                     SEYRNREFLASLNSLGVIRENIILLPETNTNGNTDFNYMEKIALTFEEKFKNVTHIAH
                     TYKLDDHLQHLKNGSVIQNLYNAGKIKDAKYFVKPKFADKITFNNKIVYKAHSTEDYE
                     KVKNACQQYKIVDELEYREGIGYKSDHKSFDKLLNNKDVPAILHTPNL"
     gene            complement(13631..14362)
                     /locus_tag="PC414_002918"
     CDS             complement(13631..14362)
                     /locus_tag="PC414_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465300.1"
                     /translation="MNIFDILRDYLLVQVDKYNLNMDMISIVSKSLSSKEAIGNTKRK
                     DFPIIVGKEIMLEADFKGAKGQAFTSTPSTFEGSLKDILSLDLHDNPHDRSLFIASLN
                     AVMKYLGKTDRTIHCKNNEPEICAKKFPEFIKMEFGNPKVAIIGYQPAIIDNIKDFFE
                     TRVLDLNPEFVDTIQYNVKIEDGIRDYEDVISWADLVICTGSTLCNNSIINFLSLNKP
                     VYYYGTTIAGASNILGLKRLCFCSK"
     gene            complement(14349..15044)
                     /locus_tag="PC414_002919"
     CDS             complement(14349..15044)
                     /locus_tag="PC414_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2465301.1"
                     /translation="MLKIKNLSLDLPNFKLQENSLNINNHEYFVLLGASGSGKTLFLE
                     TLAGRYPSAKGNVSYKENLISNLPPENRNIGFVYQNFELFPNMNVEDNIRFPLKLRKI
                     PIDEQILKANHLSKLMQIEDIKNRYPKNLSGGEKQRVAFARALIAEPDILLLDEPMSS
                     LDYITKKHISKILKDVYIKYKPTVIHVTHDISEAMFFAHKIGIMNNGKINHIFDLNEE
                     IKNKGESFFYEYI"
     gene            complement(15046..15837)
                     /locus_tag="PC414_002920"
     CDS             complement(15046..15837)
                     /locus_tag="PC414_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2465302.1"
                     /translation="MKNKFFTTLLTGFFAIVFLFIVSPLLMLIIKGISYVPICLKSVE
                     VQYAIILSIKTSLISTIVCLLLAIPVAYFLHITKLPFKKLIIQIINLPMSLPHLVSGI
                     ALLLLFGRMGIGDSIYKIFKLDFIFTKQGIVLAQVFVNLPLTIKILYTSLNESNEKMI
                     FVARTLGCNSWEAFRFIILPNLKTGIISATVMTWSRALGEFGAVAMIAGSTRMKTEII
                     PTSIYLNMSTGDIDIAIGIAVILIFISLTCLMLFEIFFNREVDKN"
     gene            complement(15847..16659)
                     /gene="modA"
                     /locus_tag="PC414_002921"
     CDS             complement(15847..16659)
                     /gene="modA"
                     /locus_tag="PC414_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2465303.1"
                     /translation="MFKKLSVLALAMIISLSITACNSKENKANDESNTKVTTADDSKE
                     KETLFVYSGAGLKKPMDEIAKKFEKENNVKVEYSYAGSAQLIAQIETSHKGDVFIVGS
                     EPIYSKAFEKELVGEHKTVAHHTPAIVVPKGNPANIQKLEDLNKDGVKLILGDKESNA
                     IGKTTQKILEKNNLKSINDNVVSTAATVNEMIVQLTSSKADATIATKDSVFGNEDVEV
                     IEIAQDKNIDQLISAGIVNYSDKKDLANKFINYIESDEAKQIFKKYGFEPVK"
BASE COUNT         6413 a         2069 c         2704 g         5645 t
ORIGIN      
        1 gttaaacata aaaaaccatt tgtaaaaaac aaaaaaatca tttctctaac aaaaaaaacc
       61 acaaaaaata taaaaaagta tggctagtta agtgattaac aagtaaaaat taaaataaaa
      121 atcaaaaaaa atttatcaat gaaagtcagc taaaatcctg gaagagagca ttatttttgg
      181 gaaaacattt tcttttgttt cacaaaaaaa gccacttttt ggcacacaac atgctttata
      241 taaagtcaag aaaaactaag gtataagaca aaggcaatta ggaggtgtaa atatggagtt
      301 tataaatttt gtaagtgata aagttataga ccttaaaatg gattgtaaaa cgaaagaaga
      361 tttctttaaa gaaatacata ataaagtttt tgatttaggc tttgtaaaag aagaatttgg
      421 agaaaaaata ttagcaagag aaaatgtatt tccaacaggt ttaaatttag gtgattatgg
      481 agtagcaata ccacatacag atgcggaata tattaaagag caatttatat cagtctgtac
      541 ttttaatgaa ccagtagtat ttagttctat ggaagaccaa gatgaaaaag tacctgtaaa
      601 tttggctttc gtattaggac ttaatcaacc acacagtcaa ttatctgtat taacagaatt
      661 aatgggaatt atgcaaaata aagagttagt tgataagttg atgagttcta ctgataaaga
      721 agaagtttta aagacactaa agtctttata aaaataaaaa agtactaaaa ataaaaaata
      781 taaaaataaa aattattagg aggaaataaa aatgaaaaaa atattagtag catgtggagc
      841 aggaatagca acatcaacag tagtttgtga caaggtagag agattagtta aggaaaataa
      901 tatacaagca gaagttatac aatgcaaaat agctgaatgt gcaacaaagc aagaaggagc
      961 agatttaata gtatcaacaa ctatactacc aacaacttat aatataccag caataaaagc
     1021 aacagcatat ataactggag taaatacagc agcattagat aaaaaaatat tagatgcttt
     1081 aaaataatcc tatcatagat acattgaaat aatattttat aaaacagaaa aaggaggatt
     1141 tataaatgga taaattatta gcagtagtac aatacatttt aaatatggga ccgacagtaa
     1201 tactaccaat aacaattgta gtactaggaa tgatatttag aattaagttt acaaaagctt
     1261 taaagtcagg tcttacaatt ggtataggat ttgtgggtat aaacttagtt gtaaacctat
     1321 taacatcaac actagggcca gcggctcaag gaatggttga aagattcgga cttaatttaa
     1381 cagtaataga tgttggttgg ccaacaacag catcagcaac atgggcttca ccagttgcac
     1441 caatattaat accagtttgt ttattagtaa acttactatt attatacttc aataaaacaa
     1501 agatattaaa cgttgatata tggaattatt ggcactttat agtagcaggt gcagttggat
     1561 atatagtaac aggaagttat gtttgggcta tattctgtgc aatactaatg gagttattag
     1621 tactgtttat tgcagaaaag acagcaaaaa atgttcagga gttttatgat ttagaaggta
     1681 tagcattacc aactggttca acagcttcat ttgctccact tggatattta gtaggtaaat
     1741 ttgttgaaaa aataccagga ataaataaaa tacaagcaga cccagactct atacaaaaaa
     1801 gatttggtat atttggagaa ccaatgatga tggggattat actaggtata atacttggag
     1861 cacttgcggg atatgatgta gcaggaatat tcaatgtagg tatatcaatg ggtgcagtaa
     1921 tgttacttat gcctagaatg gttagaatat taatggaagg tttaattcca atttcagaat
     1981 caataagaga tttcttacaa gcaaaatatg gagacagaga actttatata ggtctggatg
     2041 cagcagtagc aactggacat ccagcagtta tatcaacagc tcttatactt gttcctataa
     2101 ctttgttctt agcagttata ctaccaggaa acaaagtttt accatttgga gacttagcga
     2161 caataccatt ttatatggca tttatagtgt gttttagaaa aggtaatata gttcaatcag
     2221 ttataacagg aacaatagta atagctatat cactttatat ggctacaaac tttgcagatg
     2281 ttcatacaca attattacaa caagcaaata tgatgccaga aggaacaaca atggttacaa
     2341 gtatagatac aggtggaaac ttgctaaaat ggggaatatt gaaattatct caattagttt
     2401 cttcatttgg aatattctag tatgtagtta atagagatta ctctttttta gtaaagtatg
     2461 cgaaaattta acatttagca gctactaaaa aagagtaata ttttttataa atttttaaaa
     2521 atacaaaata caagaggagg agatttatcg tgaaagcagc agtattacat ggcacaaatg
     2581 atatgagatt tgaagatata gaaataaaac catgtgaaag tgatgaagta aaaataaaag
     2641 ttatggcagc aggaatatgt gggtcagatc ctccaagagt attaaaacat tggaaatatc
     2701 cagttccagc tataccagga catgagtttt ctggagtaat tgctgaagtt ggtaaggatg
     2761 taaaaaatgt aaaagttgga gatagagtag ttgcaattcc ttttatacct tgtaatgaat
     2821 gtgaatattg taaaagagga ttattttcat tgtgtgatga ccatggaatg ttaggagcta
     2881 aaagttttgg agcttttgca gagtatgtaa atataaaagc tacaaatgta cttccaatag
     2941 gagatatgaa ctttgaagat gctgcaatga tagagccact agcagttgct atgcatggag
     3001 tacttaatat aggtgttcaa gttggagaca ctgtagctgt aatgggtagt ggaacaatgg
     3061 ggcagttggt aatacaagga cttaaaatag caggggcagg aacaattata gcagttgata
     3121 tatctgataa taaattaaga gaatcaaagg aattaggggc tgatataata attaatgcta
     3181 aagatattaa cccagtagaa aaaataaaag aacttactgg tggaaaaggt gttgacatag
     3241 ctcttgaatg tgcaggttca aaaataactc aagaacaatg tttacttata actaaaaaga
     3301 aaagtaagat aggattttta gggatagcat attcagatat aactttatca gaagaagcat
     3361 ttgaaaatat atttagaaaa gaactagaat taaaaggttt ttggaactct tattctgcac
     3421 catttccagg acaagaatgg actaagggaa tcaatctagt taatgaagga aagataaaat
     3481 taaaagaaat ggtatctcat agattctcac ttgaagatac ttacaaagca tttgaaatga
     3541 taagagatag aaaagaagaa tttaacaaaa tattaatttt accacaaggg gttgaaaaat
     3601 aatgaaagca gtagttaaaa ctaaaccagg ctatgataat gtagaagttt tagaagtaga
     3661 agaaccaaaa gctacaggag acaaggttaa aataaaagta gaatatagtg gtatatgtgg
     3721 ttcagatata cattcattta aaggtgaata tgcaaatata aaagccccag taacattagg
     3781 tcatgaattt tctggaattg ttgtagaagt aggagaagat gttaaaaata taaaagtagg
     3841 agatagagtt acatcagaga ctacttttgc aacttgtgag aaatgtatat attgtgaaac
     3901 taaagattat aacttatgcc caactagaaa aggtattggt actcaagtaa atggaagttt
     3961 tgctgagtat gtattatcaa gagaagaaag catacatgta cttcctgaaa atgtatcttt
     4021 attatcagca gcccttacag agccactagc ttgttgtgtt catgcagctc ttgaaaagac
     4081 tactatagaa agttctgata cagttttaat aataggacca ggaccaattg gtttattatt
     4141 agctcaagtt gttaaatctc aaggtgctac agttataatg tctggtgtta caaaagataa
     4201 ggaaagatta gaaatagcta aaaatcttgg agtagataga actgtaaatg taatggaaga
     4261 aagtttggca gatgtagtga atgaaatgac tgatggaaat ggagtaaaca aaggatttga
     4321 ttgttctggg tttatgccag cagttaacga agccttaaga ctgcttagaa aaaaaggtac
     4381 tttcgtacaa gttggaatct ttgctaaaaa attaaatgaa atggaccaag aagcaataat
     4441 ccaaagagag ttacaatata taggaagcag gtctcaaaag ccaagttctt ggattacagc
     4501 attagattta atggcaaatg ggaaagtaaa tacagaagct ttagttacta gaactgttgg
     4561 tttagacgaa tttaaagaag gtattacagc tttaatgaac ggggaagaaa taaaagtagc
     4621 agtaaaatct taaaaaaaca tttgtattat tttaaaaaaa attgtaaaat ctactgtagg
     4681 ataaaaaaat gtaaggtctt aaaaaataac aggaggaaga aagtatgagc actcttagag
     4741 atcattctaa taatattaga aaaaacataa taaaaatggt tgcagaagct aaaagcggac
     4801 atccaggtgg ttcattatca atagctgata tgttgacagt attatatttt gataagatga
     4861 acgtatgtgc agaaaatcca aaaatggctg atagagatag atttgttcta tcaaaaggtc
     4921 atgcagcacc agctttatat gctacattag cagaaaaagg atttttccca gaagaagaat
     4981 taataacact aagaaaattt ggttctaagt tacaaggtca cccagacatg aaaaaagtag
     5041 caggaatcga tatgtctact ggttctcttg gtcaagggct atctgcagca aatggtatgg
     5101 cacttgcagg aaaattagat aacaaagact atacagtata tactatatta ggtgatggag
     5161 aaatccaaga aggtcaaata tgggaagctg caatgacagc atcacattac aaattagata
     5221 atttaattgc atttgttgac ttaaatggat tacaaataga tggttctaat gaagaagtaa
     5281 tgaatgtaag tccagtagat gacaaattca aatcttttgg atggaatgta atagtaataa
     5341 atggacattc ttttgaagaa ataggaaatg cagttgatga agctaaaaaa gttacaggaa
     5401 aaccaactgt aataatagct aaaacagtaa aaggtaaagg tgtttcattt atggaaaata
     5461 atgctgcatg gcatggaaca gctccaaatg cagaacaagc agagcaagca ttgaaagatt
     5521 tagaaggagg tctataggaa tgggaatagc aactagagaa gcttacggac aagtgttaaa
     5581 agaacttgca gaaaataaag atatagtagt attagatgca gatttaggaa aagctacaaa
     5641 aagtatatca tttaaagaag ttgccccaga tagatttttt gacatgggga tagcagaagg
     5701 tgacatgatt ggtacagcag caggtcttgc tacttgtgga aaaatacctt ttgcgagtac
     5761 ttttgcaata tttgcagcag gaagaggcta tgagcaaata agaaactctg tagcatatcc
     5821 aaatttaaat gtaaaaatag cagctactca tgcaggtgta acagtaggtg aagatggtgg
     5881 tagccatcaa gctatagagg atatatctct aatgagaggt ataccaaata tggttgtatt
     5941 aaatccagca gatgctttag aagcaagaca agctattctt acatctattg attataatgg
     6001 acctgtttat ataagattag gtagagctgc aactcctgat gtaaacagtg aaaattataa
     6061 atttgaaata ggaaaaggaa ctgttttaag agaaggttct gacataacag taatagctac
     6121 aggaataatg gtagcaaaag ccttagaagc agcagaagaa cttgctaaag aaggtataaa
     6181 tgtagaagtt gttaatatat ctacaataaa accattagat gaagcattaa taaaagaaac
     6241 tgctaagaaa actggaaaag tagttacagc agaggagcac agtataatag gtggtttagg
     6301 ttcagctgta tgtgaagctt tagcagaaac taaagatgta gtagttagaa gaattggtgt
     6361 taaagatgta tttggtcaat caggtacacc agctgattta ttaaaacact atggtttaac
     6421 tacagaagat atagtaaaaa atataaaaga gttgttatag atataaatat atctaaataa
     6481 caaattgatt tgacataagt caattaaagc tgtcttaaaa atagaaagaa atttctacaa
     6541 tgggacagct ttatttttac aatactttat ttttatgata attataaaat agtctgaaag
     6601 taatcaaaaa aattataaaa agaggtgcta tttaatgaaa atatctattg gatgtgacca
     6661 tggtggttat gaattaaagg aatatataaa ggaaaattta ataaataaag gtatagaagt
     6721 tgaagatgtg ggtacaaatt caaaagaaag tgtagatttt ccaatatatg caaaaaaggt
     6781 aagtcaaaat gttcaaagta aaaaatctga tttaggcata ctttgttgtg gaacaggaat
     6841 aggtatgtct attgcagcaa ataagtttaa aggaatcaga gcagctgttg tttctgactg
     6901 tttttcagct aaggcaacta gagaacacaa taatacaaat gtactttgtt taggagaaag
     6961 agttattggg aaaggattag caatgagaat tgttgaagaa tggttaaatt caagttataa
     7021 tggagaaaga catgcaagaa gattacaaat gattgatgat atagaaaaag aaaatttata
     7081 aatatataat ttataacaag ggggagcgtt atgtctatta tttgtgcttc aataatgtgt
     7141 gcagaccaaa tgaagttaaa agaggaatta gaagctcttg aagaagcagg tgtaaaatta
     7201 cttcattgtg atgttatgga tggaatattt gttaaaaact tggctatggg accagagtta
     7261 ttaaaatcaa taagtgaaaa tacaacaata cctttagaca tacacttagc cacagaaact
     7321 ccagataaat atattgatat gatgtcttat ataaagccaa aatatatttc ttttcatgta
     7381 gaatcaagta caaatgtaaa agctgatata caaaagttaa gaaattatgg tatagggcca
     7441 gtgttagcaa taagccctca aacaagtgtg gataaaatag aagaatatat aagtttagtt
     7501 gagggtatct tagtaatgac agtaaatcca gggtttgcag gtcaaaagtt taatctttct
     7561 gtattggata agttggataa acttacagag atactaaaag attatgataa tcctccattt
     7621 atagaggttg atggaaatat aaataaggat actattaaac ttatgaatgg taaaaaagta
     7681 gatatatatg ttgttggtac ttcagcttta tttaatgata agcctccaat ttcatataaa
     7741 gataaaatag aagaactaaa agaaagtata aagtaaccat gtaataaaat tataatatgt
     7801 ttctgtttaa tagtaaaaat ttaggtggaa acaagaaatt ttataatatt taataaaaat
     7861 tttggttgag taaggagaat tttttatggg agacctatat aaactacaaa atggtactga
     7921 tattagagga atagcgtatg aaaatgattc taaagaagtt aacctaactg tagaagaagt
     7981 aaaaaaaata gctaaagcgt ttcatatttg gttaaaagaa aagactaaga aagataaagt
     8041 aactgttgcc ataggaactg attctaggat aacaggaagc caatttagaa gtactgttat
     8101 tgaaacttta actaatgacg attgtaatgt aattgattgt gagattgcca caactccagc
     8161 tatgtttatg acaactatta tggatggata caattctgat ggggcgatta tgattacagc
     8221 aagtcactta ccttattact ataatggttt aaagtttttc acagaaagtg gaggtttaga
     8281 aaaaactgat ataaaagaga tgttggatat agctgttaaa aatgatagtg tagactatga
     8341 ggaagtaaat aaaaaaggtg aagttatagt aaaaaattta atagaagatt attctaattt
     8401 attgatagat aaaattagaa aaggtgtaaa ttcaagtaaa aattatgaaa agcctttttc
     8461 tgggcttaag atattggttg atgcaggaaa tggggctggt ggattctttg ctgaaaaagt
     8521 tttacacata ctgggagcag atacgacagg tagtcagttt ttgaacccag atggtatgtt
     8581 cccaaatcat atacctaatc ccgaaaacaa agaagcaatg gaaagcatat gtaaagctgt
     8641 gttagataat aagagcgacc taggaataat ctttgataca gatgtagaca gagctgctat
     8701 tgtaggtaaa aatggcaaac caataaataa aaatgctctt atagctgtta tatcatcaat
     8761 agtattagaa gaacatccaa aaacagctat agtaacagat tcgattactt ctgaaggact
     8821 tgctaaattt ataaatgaat tacaaggaag acatcataga tttaaaagag gatataaaaa
     8881 tgtaataaat gaagctataa gattgaatag tgaaggtgaa gagtgccatt tagctataga
     8941 aacgtctgga catgctgctt taaaagaaaa ttatttctta gatgatgggg catatttaat
     9001 agctaaaatt ttaataaaag tagcaaagct atccttggaa ggaaagacta tagaagaatt
     9061 gatagaaaac cttgaagaag ctcaagaaga gaaagaagtt agaataggca ttaataaaga
     9121 agattttaga ccatatgcag aggatatatt aaaagattta gattctcatg tcaagagtat
     9181 tgatggatgg tctgtagcac caaataattt tgaaggcata agagttaatt gtgatgaatc
     9241 aaatggagat ggatggtttt tacttagaat atcactacat gaaccattat tagctttaaa
     9301 tattgaatca aataaaattg gcggagcaga acaaatttat tctaagttaa aacttttctt
     9361 agaaaaatat gatttaaaag gtctttaggg taaatagaaa gaatatattt atattgaaga
     9421 ataaatgtaa atttgtacct aagattacaa atattacaaa attgtaatct taggtaacag
     9481 agatgtaact gattatggaa acactatgtg atattataga tacagagaga tgaaatatgt
     9541 gatgaattat agagaaatat aatttaaaat gtattaatat cacaccaaaa aggtataatc
     9601 aaaaaatatg taaatataaa ccccaacatt tagaagaaga cctctaagga ggtttttttc
     9661 ttgtttaaat aacttaaaat atatttttat atcaatatat caattaactc ttctaaaagt
     9721 aagcattacc ttaaataaat cagcatctat aattatttcc atgtgtgcat tttctaattc
     9781 aattatattt tgagcaattg ataaaccaag accagagcca tctgtttttc tagatatatc
     9841 accccttcta aatctttcta ttaactcttc tggctttaga tttagtttat catttgaaat
     9901 atttttaaac gttaatatta tattttcttc ctttataaac atatctatgt agaccctagt
     9961 gttaggcata gagtatttta gtatattgca aattagattc tcaaaaactc tccaagtttt
    10021 tttaccatct atcatggtaa aaacagcata ttcaggtgtt tctattataa aatctaaact
    10081 gctattttga attttgtctt caaactctac aatactttga attaaaagtt ctcttatatc
    10141 ggtttttgtt atattaaatt ctacatctcc actacttagc ttagatgctt caaatatgtc
    10201 atctattaat aattttaatc tttgtgattt tttatctaaa atatttatat agtcatttat
    10261 agtctctctg tcaaagtcat tattttttaa catatcaaca tagcttaata tagatgtaag
    10321 tggagttttt aagtcatgag atacgtttgt gataagctct gccttcatcc tttcattttt
    10381 tatattagca tctacagcag tttcaagacc acctgttata ttatttatac ttttggctaa
    10441 agtagatatt tcatcatctc ctataacttc taatttatac tttaaattgc cattttctat
    10501 attttttata ttttttgata tgttatttat gtaattaaac ttaacagata aaaagttgta
    10561 tatataaaaa tgaaaaactg tgtttacaag tgtaaataaa attactatag ctattgcctc
    10621 agcataatat atatacataa agaatatcaa aacaagatat tggataatag cttgtacaac
    10681 tatagatatt aaaaatacta tagctaattt tttatttata gtttttttat tatacatatt
    10741 aagcaagttt gaaaaaaata catatgttat agacatatct tttagattat agttattata
    10801 tttaatattt tcatatatta tatagactat agatataatt ataaaattgt agattattaa
    10861 attagtgttg taaaacattt tattacttgt aaatgcatga aaccttaagg ttaatagtat
    10921 caaagttaat ataagtgtaa gttctatttt taccttagaa aaaaggctta tgacattctc
    10981 taagaatttg actttagtct gtttattttt tttatacaat gtaaaacata gtatagtcaa
    11041 taaaaaactt gataatataa tacctatgca gacaaactct aattctactt ttgacaaatg
    11101 tacaggagta ccgaaaaaat aaaaataagg gagagcaaac tctttataat aataattaat
    11161 attataagaa aagttattat tcaaatcata aactgcttca aataatatag aaactaatag
    11221 aaacagtaat aataataaaa taaattttgc tttagaatca ttaataatta agtttttcaa
    11281 ttttgtaacc aatgccccac accaccttta aatattttgg ttcttttgga ttaatttcaa
    11341 tcttttctct aattcttcta atatgaacag caatagttct ctcacaacta taagcatcct
    11401 cattccagac attttcataa atctcatgag aagagaaaat tttacctttt cgtgacataa
    11461 gaagtgtaag tattttatat tcaagtggtg tcattttaat ttcttcacca tctaaagaaa
    11521 ctatttttgt atctgtattt agctctaggc caccactttt tagaatataa ttagtatcat
    11581 ttgagttatt tgtattacta aaatcaccta aatttatata ccttctaagt tgagatttta
    11641 ctcgagcagt aagttctagt ggattaaatg gtttagttat atagtcatca gcacccatat
    11701 taagacctat aattttgtcc atatcttctg attttgcaga taagagaatt attggtatat
    11761 tccttttttc tcttatttta agtgtagttt taagcccatc taaattgggc atcattacat
    11821 ccattattat taacttaata tcattttcga ttagtatgtc caatgcttca agtccatcat
    11881 atgcctttaa aacattaaag ttatcaggtc tcaaatagaa ctctatagca tcaactatct
    11941 ctctatcatc atctacaacc aatatatttt gattcaactt attaaccccc ttgaaatttt
    12001 atttttaacc aatttttatt ttataccaaa aagaggtatt agaaaacagt aagcccgttc
    12061 attatgaaat aaaaactagt acttttaata tattgtcaat gcatatagta aatttaaaag
    12121 tatagacgcc catattaatc tgaaggtaaa agtttatgaa aatatctagg gtaatctatc
    12181 acactcctta tgttattttg gtttattaat tttttaggtt taaacatata ttaacaaata
    12241 taaataacaa atgagtttca taattattaa gaatttataa caaatattta ttttaatgta
    12301 gtttaatata aataggtagc ttaatgtaga tatgtataaa gcatttaata aggcttatag
    12361 gttttaatga tgatatcttt atatatttta agagatgatg aaattagata tgggtaatag
    12421 ttgtatgaga aaaagtagta tagcacaaaa agtgctatat cacaaaatct caaataattt
    12481 cctttttata acttaaatta aaagaataaa tatttcttat aaattaggtg tatgaagtat
    12541 agcaggaaca tctttattat ttaataactt atcaaatgat ttatggtcag acttatagcc
    12601 aataccttct ctatattcta attcatcaac aattttgtat tgttgacagg catttttaac
    12661 cttttcatag tcttcagtag aatgagcttt ataaactatc ttattattaa aagttatttt
    12721 atcagcaaat ttaggtttta caaaatattt agcatctttt attttaccag cattatataa
    12781 attttggata acagaaccat tttttaaatg ttgtaaatgg tcatctaact tataagtatg
    12841 tgctatatga gttacatttt taaatttttc ttcaaatgta agagcaattt tttccatgta
    12901 attgaagtca gtattaccat ttgtatttgt ttctggtaat aatattatat tttcccttat
    12961 tacaccaagg ctattaagag aagctaaaaa ttctctattt ctatattctg atttttgtat
    13021 atttgttagg ttttcatatt ttttatattt atcaaagacg gatataccac atccagtaga
    13081 tactaaaacc acaaatacat gattttttcc tctttctttt atagcattaa caatagcact
    13141 cccagcccac aacacttcat catcttgatg ctgaacatag aagacaacat aatctttaaa
    13201 ttttttatga ctataattca catagctact aaaagcggtt aaaatcaaac ataaaactag
    13261 aataatagat agaattttat atttatttat agtaaatttt ttattcttaa atgtcattta
    13321 atcatctcct gaaaagttgt cttagttata ttatcaaaca aagtactaaa aattatttct
    13381 taaatgctga aaaatataga gtagattaat ggaataaaat ttttttatta gtttaaaata
    13441 aagaaaacat ttatgtataa gactaatcaa aatattaatg attgatagga attgagattt
    13501 aaagggattg tacatttatt cgggattcat tttaatagaa ttttacttta gtacttagaa
    13561 taaaagtaat aactttttaa atttaaagag tacacatata tatttgtgta ctccttgatg
    13621 atatgattgc ttatttacta caaaaacaga gtctttttaa acctaaaata tttgatgcac
    13681 cagctatcgt agttccataa tagtaaactg gtttgttaag tgatagaaaa tttataattg
    13741 agttattaca taatgtactt cctgtgcaaa tcactaaatc agcccaagat attacatctt
    13801 cataatctcg tataccatct tcaatcttaa cattatattg gatagtatcc acaaattcag
    13861 gatttaaatc taatactctt gtttcgaaaa agtccttaat attatcaatt atggctggtt
    13921 gatatcctat aattgctact tttggattac caaattccat ttttataaac tcagggaatt
    13981 tttttgcaca tatctctggc tcgttatttt tacaatgaat ggttctatcc gtcttgccta
    14041 agtatttcat aactgcattt aatgatgcta taaaaagact tctatcatga ggattgtcat
    14101 gtaaatctaa tgataatatg tcttttaaag aaccttcaaa tgtagatgga gtagaagtaa
    14161 atgcttgtcc tttagctcct ttaaagtcag cttcaagcat tatttcttta ccaactataa
    14221 ttgggaaatc tttacgctta gtatttccaa ttgcttcttt tgaagataga gatttggaca
    14281 caatacttat catatccata tttaaattat atttatcaac ttgtactaat agataatctc
    14341 tcaaaatatc aaatatattc atagaaaaaa gattctcctt tattttttat ttcttcattt
    14401 aaatcaaaaa tatgatttat ttttccattg ttcattattc ctatcttatg agcaaaaaac
    14461 attgcttcag aaatatcatg agtaacatga atcactgttg gtttgtattt tatgtagaca
    14521 tccttcaaaa ttttgcttat atgttttttt gtaatataat ccaatgaact cataggttca
    14581 tctaaaagta atatatcagg ttcagctatt aaggcacgtg caaaagcaac tcgttgcttt
    14641 tcacctccac ttaaattttt aggataccta ttttttatat cttctatctg cattaattta
    14701 gataagtggt tagccttaag aatctgttca tctataggta tttttctcaa tttaagtgga
    14761 aaccttatat tatcttcaac attcatattt ggaaataatt caaaattttg atatacaaat
    14821 cctatatttc tattttctgg aggtaagttt gatattaaat tttctttgta tgatacattt
    14881 cctttagcag atggatatct tcctgctaat gtttctagaa atagagtttt acctgaccca
    14941 ctagctccta ataaaacaaa gtattcatga ttattaatat ttaaactatt ttcttgtaat
    15001 ttaaaattag gtaaatcaag agataaattt tttattttta acatattaat tcttatcaac
    15061 ctctctatta aaaaatatct caaaaagcat caagcatgta agtgagataa atatcaaaat
    15121 tacagctatt ccgattgcaa tgtctatatc accagtagac atatttaaat atatagatgt
    15181 aggaataatt tcagttttca tacgtgtaga acctgctatc atagctactg caccaaattc
    15241 accaagtgct cttgaccaag tcattacagt tgcagatatg attccagttt ttagatttgg
    15301 aagaattata aatctaaatg cttcccaact attacatcca agggtacgtg caacaaatat
    15361 catcttttca ttactttcat ttagtgaagt gtaaagtatt ttaatagtaa gtggaagatt
    15421 tacaaatact tgtgctaaaa ctataccttg ctttgtaaat ataaaatcaa gcttaaaaat
    15481 cttataaatg gaatctccaa tccccattct tccaaacaaa agtaaaagag caattcctga
    15541 tactaagtgt ggaagagaca tagggagatt tattatctgt ataatcagtt ttttaaatgg
    15601 taatttagta atatgtaaaa aatatgctac tggaatagct aataaaaggc atactatagt
    15661 agatattaaa gatgttttaa tactaagtat tattgcatac tgtacttcca ctgattttaa
    15721 gcatatagga acataactta tccctttaat gattaacata agaagtggtg atacaataaa
    15781 caaaaagact atagcaaaaa atccagtaag tagagtagta aaaaatttat ttttcaagtt
    15841 taatcactac ttaacaggtt caaaaccata ttttttaaat atctgtttag cttcgtctga
    15901 ctctatataa tttataaatt tattagctaa atctttttta tcactatagt ttacgattcc
    15961 tgctgatata agttggtcta tatttttatc ttgggcaatt tctataactt ctacatcttc
    16021 attgccaaag accgaatctt ttgtggctat tgtagcatca gctttacttg atgttaactg
    16081 tacaatcatt tcatttacag ttgcagctgt agagacaaca ttatcattta tagattttaa
    16141 attatttttt tcaagtattt tttgtgtagt cttacctata gcattagact ctttgtctcc
    16201 caaaataagt ttaactccat ctttatttaa gtcttccaat ttttgaatat tagctggatt
    16261 tcctttaggt acaactattg caggtgtatg atgtgcaacc gttttatgtt cacctacaag
    16321 ttctttttca aaggcttttg aataaattgg ttcagaacca acaataaata cgtccccttt
    16381 gtgacttgtc tcaatttgag ctattagctg tgcagaacct gcatagctat attcaacttt
    16441 gacattattt tctttctcaa atttttttgc tatttcatcc attggtttct ttaaacctgc
    16501 ccctgaatag acaaaaagag tctctttttc ttttgaatca tcagctgttg tcactttagt
    16561 attagattca tcattagctt tgttttcttt agaattacat gcagtaatac ttaatgaaat
    16621 aatcatagct aaagctagta ctgaaagttt tttaaacata agatattccc ccttagtaaa
    16681 ttaatgtaaa ttctttttta gtatatcata ctaagaaaac gatgtctaag tttttgttat
    16741 gttttataat gttttgttat gttttgttat gtatagttaa gaaaaaatct atttataatg
    16801 ttatttctat aggtagttat tttattttta a
//
LOCUS       DAMJVK010000090        16597 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.092, whole genome shotgun sequence.
ACCESSION   DAMJVK010000090 DAMJVK010000000
VERSION     DAMJVK010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16597
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.092"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            161..829
                     /locus_tag="PC414_002922"
     CDS             161..829
                     /locus_tag="PC414_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465304.1"
                     /translation="MSKLESTTEKVEVKWYGYVALILGALFFSGIFKDAPGPLKVLDF
                     NNVLGSFGTLGTINDGVGTLAANFRGDGGTGPRDGWLYALTLIPSVMFALGVVRVIDH
                     LDGMKAAQKLLSPLLKPLLGLPGFAGLTLIASLQSTDAAASMTKELKDDGYIDEKQKA
                     VFCAFQFSGASAITNFFASGAALFPFIGDVPIFIPLALILIMKFVGANLLRLYLNKFD
                     KGEA"
     gene            831..1316
                     /locus_tag="PC414_002923"
     CDS             831..1316
                     /locus_tag="PC414_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HDF2465305.1"
                     /translation="MSEVAKKEVKVKKGNVIDAFIGGARNGFQISTNSMAPNVIFGFA
                     IISVFNLTGLLDVIGTIFTPVMSIFGLPGVAATAIMTIFISMGGASGVIAGLFTAGQI
                     SQAHVAILLPMLLLSGAQIQFMGRLLGTAELKTKYYPHMFVIATLNGCIAMLIMNYFV
                     V"
     gene            1377..2498
                     /locus_tag="PC414_002924"
     CDS             1377..2498
                     /locus_tag="PC414_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 peptidase aminoacylase family protein"
                     /protein_id="HDF2465306.1"
                     /translation="MGTNKDYIEYVHKVYDYLHTIPEKGFEEYKTSEFVLKELEKMGF
                     ENINKGVAGTGIIVTIDSKKEGPVFAIRADMDALEFIIDGEKKMIHACGHDGHTSMLL
                     TAAKLIKERNLVKRGKLKILFQPGEEKLYGALRVIDSGLLDDVEEMVGMHLRPGQDCR
                     LGKAIPAMYHCASCIMEFTIKGITCHGSKPHLGVNAVDAVCLAVNAINAIRVNPAIPN
                     SIKVTNISVKGETYNNIPDEAFMALDIRAESNDIIEELKEKAIHAVKTVVSSINAEAV
                     LISKDGVPAAEFDEELVNLAEETIKDVLGADASIGIFKNSGGEDFHYYTQKLKCKTTY
                     IGLGADATPGFHNPNVTFDTKALEYGVDIWCRLVEKRLG"
     gene            2779..3969
                     /locus_tag="PC414_002925"
     CDS             2779..3969
                     /locus_tag="PC414_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2465307.1"
                     /translation="MSKYNFDRVIDRVGTDCVKWDFRTNCSTKAQKDGLPFWIADMDF
                     ECAEPIIEALRKKVEHKIFGYSSNDSDKYFNAVCDWYKRRFNWEISRKDIIFSPGVVP
                     AVAILVRILTNSNEGVIVQKPVYYPFEAKIKSNNRDVVNNPLIYENGTYRMDYDDLEE
                     KAKCSNNKVLILCSPHNPVGRVWREDELKKVVEICKKYDLWIIADEIHSDLIRKGFKH
                     TPLQSLCPEYKDKIVTCIAPSKTFNLAGMQLSNIIVNNDELKKKYQEEVTAVGVATSP
                     NPFAIVATIAAYNESEDWLNELNDYLDNNIQFIDEYLKENLPKVKLVYPEGTYLAWLD
                     FTAYGLNEVELEDLMFKKANVLFDEGYIFGKEGIGFERINVACPQSLLKECMDRLKTI
                     FENL"
     misc_binding    4103..4358
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4417..5418
                     /locus_tag="PC414_002926"
     CDS             4417..5418
                     /locus_tag="PC414_002926"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HDF2465308.1"
                     /translation="MSTIIPKDYKSSLNVIDTQIAIKKLKDFFEMRLSNELNLIRVSS
                     PLFVLPETGANDNLNGEKAVSFDIPFMNKNAEIVQSLAKWKRLALKKYGFEVGSGLYT
                     DMNAIRKNEELDNIHSLYVDQWDWELVIDKSSRNEKTLKDVVKRLYGVFKDTEIFVCS
                     MYEGIKEILPEEITFITSQELEDMYPELSPKEREDKIVKEKKAVFITQIGKTLISGEK
                     HDGRSPDYDDWELNGDILFYNPVLDSALELSSMGIRVDEESLDRQLKLAGCEERKEFD
                     YHKMLLNGELPYTIGGGIGQSRICMYFLQKAHIGEVQVGVWPQDMIQNCSSAGIELL"
     gene            5522..5911
                     /locus_tag="PC414_002927"
     CDS             5522..5911
                     /locus_tag="PC414_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HDF2465309.1"
                     /translation="MEFLKIIINIVLDILKKILVRFKNAKFGLVFVFDLLKLPDFMTD
                     KRINIVDKIKVISVLIFTISYFVSGVDIIPEMIAGAFGFIDDAIVLIWSIGIVNEEIN
                     KYRVIAKKDKHSNIIENVEFSIKDEEE"
     gene            6072..7280
                     /locus_tag="PC414_002928"
     CDS             6072..7280
                     /locus_tag="PC414_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HDF2465310.1"
                     /translation="MKKKIGLVPKLIIGIIVGILIGSYAPEIIVQILVTVSTLFSAFL
                     KFVIPFIIIGFVTAGIADLATGAGKLLGITTGIAYGSTLIAGLLSFAVSTLIFPNFID
                     ASVASQIGDPEAGMLAPIFTIPLEPMVDVTAAIVFAFVMGLGISALRNHGKGETLFNF
                     FQEFQGIVTKTLSTVIIPLLPLYIAGTFANITIAGEVWTILGVFWKVYLVVIPLHFVY
                     MACQFTAAGVFSGKNPVRMLKNQVPGYLTAIGTQSSAATIPVNVGCAEKNGVSKQIRE
                     FVVPLCATIHLSGSMISITGFTTAVLMMNGMPHGIGVLFPYIAMLGIAMVAAPGAPGG
                     AIMSALPFLPMVGIPSDGGLASLMIALYLTQDSFGTACNVSGDNAIAVVVDKINDGMS
                     KKGNKEEQSA"
     gene            complement(7366..8442)
                     /locus_tag="PC414_002929"
     CDS             complement(7366..8442)
                     /locus_tag="PC414_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF2465311.1"
                     /translation="MSTNHGANLYSLSSKYGFSKEEFMDFSSNINPFGTSSLAKQYIV
                     NNIDMVSMYPDPDYIDLKTSISNYCKCSIDNIVLGSGATELISSFIHTINPKQALLLS
                     PAYSEYEKELSKINCSIEKYFAKEEDNFHINLENLIKTINAKDYDLVVICNPSNPTGF
                     AFTKVEVREILKNTDSFLMIDETYVEFTDTDTYSCTQLVDDYSNLFVIRGTSKFFSTP
                     GIRLGYGLISNTNVKNEINKNLDLWNINIIASKMGEIMFSDLDFISNTISLMNTERDY
                     LLKELKNIKSLDIYNTKGNFILCKIKTKELTAKSLREQLLPQKIIIRDCCSFEGLDEY
                     FFRVCILKPNENKLLISSLKAIFK"
     gene            8671..9198
                     /gene="hpt"
                     /locus_tag="PC414_002930"
     CDS             8671..9198
                     /gene="hpt"
                     /locus_tag="PC414_002930"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDF2465312.1"
                     /translation="MYKVTGKMLTEEQIKEKVYELGKKIEEDFKGEDLLVVGILKGAS
                     VFVSDLIRCIDLDVNIDFMSVTSYGNSTESSGTVKILKDLDVDIEGKNVLIVEDIIDS
                     GLTLSNLVAALKTRNPKSLKLCTLLDKPQRRKANIPVDYVGFVIEDKFIVGYGIDYAE
                     KYRNLPYIGIVEDVE"
     gene            9287..10219
                     /locus_tag="PC414_002931"
     CDS             9287..10219
                     /locus_tag="PC414_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDF2465313.1"
                     /translation="MDIQLNILLFFQNLRNPILNFIFLVFTISTEAPLLILITTIIYW
                     CINKKCGQKILFAIIGNFVVNLGIKEFVKAPRPIGIKGLESLRVSTAGGYSFPSAHTQ
                     TATTFWVSIMTIFKKRYLHLIGAVMVLGVGLSRLYLAVHWPIDVIAGWILGIFFTVVF
                     VKIFDYIDDNKNYILLVVLLIPFIIVAIFLNSPEYFEKFGIIVGFVFGYMVEDRFVKF
                     NTDNNNKKINFSNKNKGSKNIIFSRICRFLVGIVTIGMLYIGIKLFISMLIVTLNISD
                     STMSIIFMNFIKNTVVVFYGIAGAPALFKLLKLN"
     gene            10281..11507
                     /locus_tag="PC414_002932"
     CDS             10281..11507
                     /locus_tag="PC414_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDF2465314.1"
                     /translation="MKKVVIIGGGPAGMIAASTACEKGYDVTLIEKNHKLGKKLAITG
                     KGRCNITNACEIEELIENVPTNGKFLYSAFYTFTNDDVISMFNNLGVKTKTERGKRVF
                     PESDKAFDIVNALERQLKSKKVNILLNSKVEKIISKNNKIEKVILNDKKEIKCDSVVV
                     ATGGLSYPLTGSTGDGYKFAISQGHTIIDTKPSLIGIEVQESFTKDLEKLSLRNVEIR
                     VFNSKQKKVYSDFGELEFTKFGLDGPIIKSASCRMKDTRKENYTILLDLKPALDEEKL
                     DKRVQKDFQKYTNKKFEKALDDLLPKKLIPIIINLSEINPNTVVHQISREQRKNLVHL
                     LKNLKFTVKRYRPIEEAIITSGGVKVNEINSSTMESKLVEGLFFAGEVIDIDAYTGGF
                     NLQIAFSTGYLAGFNC"
     gene            11591..11812
                     /locus_tag="PC414_002933"
     CDS             11591..11812
                     /locus_tag="PC414_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HDF2465315.1"
                     /translation="MASNNNNNRTVVPGAKEALNQMKLEIANELGMSNYQQVDKGNLT
                     ARENGYVGGYMTKKLVEMAEQQMAGKSNR"
     gene            12001..12171
                     /locus_tag="PC414_002934"
     CDS             12001..12171
                     /locus_tag="PC414_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465316.1"
                     /translation="MSSVNFEDLKNKFINSDLDEKIRIYTTTEGLSVEQFKELLKYYP
                     IQHLSKLEKALG"
     gene            complement(12264..12725)
                     /locus_tag="PC414_002935"
     CDS             complement(12264..12725)
                     /locus_tag="PC414_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465317.1"
                     /translation="MQIEQLDLETRNKIYCYTKKILRKYQKGITSGKLTADKFADNIL
                     SQDFISSILNEKIVNETNFKISYRNYIETLINIQNENLSNLRKKSTKTAKCFNISQIT
                     QLKNLLSNTGYNLLIPYKYLTARDIEGIVTLINTGSIELGNERIYNYISKV"
     gene            12909..13376
                     /locus_tag="PC414_002936"
     CDS             12909..13376
                     /locus_tag="PC414_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465318.1"
                     /translation="MKMLNGIIKLFYSEIVKTKINNLDSSGYSLRDECKEIRNITLKI
                     DSVFFKLLKLAFGSILLMVAFNLNMVLGIGVFLVGVVYILYKRNMEEQVKEHINNVKN
                     NIDISKITFMDEKGRTGLNALITLLLIGLLTGFNWILVASFVVVFVFTIKNVC"
     gene            13419..15182
                     /locus_tag="PC414_002937"
     CDS             13419..15182
                     /locus_tag="PC414_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HDF2465319.1"
                     /translation="MTNGSYKMKNLVEISAMVTESTDFFNIKDDIIEKMLEVVHPTKA
                     CVNLFYKNDSKYAYLVCSQTLEYVPQIFPINSLKGAKIDFDTYPEYIHEAVNEKKIIY
                     VENVFEDSRAEGERDLAKAEGYIGRIVFPFIINNVVVGFMTCFLKEDDYITEQDIDFI
                     SSVASLLSLSIEITNKNNNVQILIDKLRGSISAINEATKKLYLNKSIDGFLENLSKQA
                     CNITNSKEALIIINSHHNKDAIVSCYNVEQKKKTNLYPMIDMFLKKESLGGYLNNLKP
                     CSKKGINLESYIYYKLQDKNDTIGCIVCANSKNYTSDDLNILSILSKQVSVGMQLYEH
                     NEKEVKHKVLENELNILNKQQQLIMDKGKMDCNDEKELYFYHKPARVVGGDFYYATKV
                     DDEHIVYIVADVMGHGMVSNYVVAMIKGAFKVLCNQYNTPREIMTNLNRMLYDEFDKM
                     GVFTTCLIGIIDTKENMITVSNAGHYSPVVVKKDGTIENNLNCKKGIPIGIMEDATYE
                     NNTLSMEDYAMVCMYTDGILEIKNSSKEEYGINRLENFLKENFRLNQQLIVENLKLDL
                     KNFSSKDNYDDDILIVMLKDR"
     gene            15190..15798
                     /locus_tag="PC414_002938"
     CDS             15190..15798
                     /locus_tag="PC414_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2465320.1"
                     /translation="MDSNDKKGLKSPEIIIINGSPRINKNCSFIANEVKAMLEECGLS
                     CKIFNIYNMDIEYCTACGFCEKTGYCKFNDDMKPLYKMFDDSKGTIVISPVHFDSISA
                     KLKTVVDRTQAIYASKYVLNKPSIDRSKKRIGMYIAVGGSKSYDSQFKGGQIVMDFFF
                     KSINTKLLHNIYLNNTDDVSYIDNQEFKDIIYKNLKIYIDSI"
     gene            15870..16439
                     /locus_tag="PC414_002939"
     CDS             15870..16439
                     /locus_tag="PC414_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HDF2465321.1"
                     /translation="MKLPIALSNKHVHLSQADIDVLFGAGHELTKFKPLSQPGQYACE
                     EKVDLVGPKGTLKGVRVLGPARAHTQVEISLADGFPLGVKAPIKESGDIAGTPGVKLV
                     GPAGEVEMKEGVIVASRHIHMSNEDAAKFGVTDKQIVKVKTSGPRALVFENVLVRASD
                     SFNLEMHVDMEEGNAAGVKNGDLVELIVD"
BASE COUNT         6488 a         1685 c         2934 g         5490 t
ORIGIN      
        1 ttaaacttga aaagtaagta attataagct atttaatcac tggaataaat ttacaataaa
       61 aaaataaata caaaggcttt atcattaagg tattggcata agttttgctt attaaacaag
      121 tatactaata aaattcattt aaacaggagg gagaatttat atgagtaaat tagaatcaac
      181 aacagaaaaa gttgaggtaa aatggtatgg gtatgtagca ttaatattgg gagcattatt
      241 tttttcagga atatttaaag atgcaccagg accattaaaa gtattggatt ttaacaatgt
      301 attaggttct tttggaacat tgggaacaat taatgatggt gtaggtacat tagctgcaaa
      361 ctttagaggt gatggaggta ctggccctcg agatggatgg ctatatgcat tgacattaat
      421 accatcagtt atgtttgcac ttggagtagt tagagttata gaccacttag atggaatgaa
      481 ggcagctcaa aagcttctta gtccactatt aaagccatta ttagggttac ctggatttgc
      541 aggactaact ttgatagcta gtttacagtc tacagatgca gctgcatcta tgactaaaga
      601 acttaaagat gatgggtata tagatgaaaa acaaaaagct gtattttgtg catttcaatt
      661 ttcaggtgcg tctgcaatta cgaatttctt tgcttctgga gcagcactat ttccatttat
      721 aggagatgta ccaatattta taccacttgc tttaatatta attatgaagt ttgttggggc
      781 gaacttactt agattatatt taaataaatt tgacaagggg gaagcttaac atgagtgagg
      841 tagcaaaaaa agaagtaaaa gttaaaaaag gaaatgtgat agatgcgttt attggaggag
      901 ctagaaatgg attccaaata agtacaaatt caatggctcc aaatgtcatc ttcggttttg
      961 ctataatttc tgtgttcaac ttaacaggtc ttttggatgt aatagggaca atatttacac
     1021 ctgtaatgag tatctttgga cttccaggtg ttgcagcaac agctataatg acaatattta
     1081 tatcaatggg aggagcatct ggagtaatcg caggtttatt tacagcaggt caaataagtc
     1141 aagcacatgt tgcaattcta ttgccaatgt tattgctttc aggagcgcag atacaattta
     1201 tggggagact tttaggaaca gctgagttaa aaactaaata ttatccacat atgtttgtta
     1261 tagcaacttt aaatggttgt attgcaatgc ttataatgaa ttatttcgta gtataggtaa
     1321 ttataataaa aatttaatat aaatggaaag tttaaatttt aaggaggagc tagattatgg
     1381 gaactaacaa agattatatt gagtatgtac ataaagtgta tgattatcta cacactatac
     1441 cagaaaaagg atttgaagaa tataagacat cagagtttgt tttaaaagag cttgaaaaaa
     1501 tggggtttga aaatattaat aaaggagttg caggaactgg tattatagta actatagatt
     1561 ctaaaaaaga aggaccagta tttgctataa gagcagatat ggatgcattg gaatttatca
     1621 tagatggaga aaagaaaatg atacatgctt gtggtcatga tggtcatact tctatgcttt
     1681 taacagctgc aaagttaata aaagagagaa atttagtaaa aagaggtaag ttaaaaatac
     1741 tttttcaacc aggtgaagaa aaattatatg gagcattaag agtaattgat tctggtttgt
     1801 tggatgatgt agaagaaatg gtagggatgc atttaagacc tggacaagat tgtaggttgg
     1861 gaaaggcgat acctgcaatg taccattgtg ctagctgtat aatggaattt acgattaaag
     1921 gtatcacttg tcatggttct aaaccacatt taggagtaaa tgcagtagat gctgtctgtt
     1981 tagcagtgaa tgcaataaat gcaataagag taaatccagc tataccaaat tctataaaag
     2041 taacaaatat atcagttaag ggagaaactt ataataatat accagatgaa gcatttatgg
     2101 ctttagatat aagggcagaa agtaatgata taatagaaga attaaaagaa aaagcaatac
     2161 atgctgtaaa aactgttgta agttctataa atgcagaagc tgtactaatt tccaaagatg
     2221 gagtaccagc agcagaattt gatgaagaac ttgtaaactt ggctgaagaa actataaaag
     2281 atgtattggg agctgatgca tctataggta tatttaaaaa ttctggtgga gaagacttcc
     2341 attattatac acaaaaacta aaatgtaaaa ctacatatat tggattagga gctgatgcta
     2401 caccaggatt tcataatcca aatgtaacat ttgatacgaa agctttagaa tatggtgtag
     2461 atatatggtg tagattggtt gaaaagagat tgggataaca tccaaactaa taattctaca
     2521 tttaaaatta taattataga agttatagaa aaagattgct tatgttaatt agaaaataat
     2581 taatatgagc aatcttattt tgatataatg aaactgattt tggaatattg aaaaaatatg
     2641 atggaaaagt aattatattt taaatctatt ggacaaaaaa gtgatattat tataatatta
     2701 agaattgtaa aataatacca aagtgcatct agtaatagac ttaacaatta taaaaatttt
     2761 attcttagga gggaaaaaat gagtaagtat aattttgaca gagtaataga tagagtagga
     2821 acagattgtg taaaatggga ttttagaact aattgttcaa caaaagcaca aaaagatggt
     2881 ttgccttttt ggattgctga tatggatttt gaatgtgcag aaccaataat agaagcatta
     2941 cgtaaaaaag ttgaacataa aatatttgga tatagttcaa atgatagtga taaatacttt
     3001 aatgcagttt gtgattggta taaaaggaga tttaactggg aaataagtag aaaagatatt
     3061 atttttagtc ctggtgttgt acctgctgtt gctattttag taagaatatt aactaattct
     3121 aatgaaggtg taatagtcca aaaaccagtg tattacccat ttgaagctaa gataaagagc
     3181 aataataggg acgttgtaaa caatcctctg atatatgaga atggtactta taggatggat
     3241 tatgatgatt tagaagaaaa agctaagtgt agcaacaata aagtactaat actttgtagt
     3301 cctcacaacc ctgttggaag agtttggaga gaagatgaat taaaaaaggt tgtagaaata
     3361 tgtaaaaaat atgacctatg gataatagct gatgagatac attctgattt aattagaaaa
     3421 ggatttaaac atactccttt acagtcatta tgcccagagt acaaggataa aatagttaca
     3481 tgtatagctc caagtaaaac atttaatctg gcaggaatgc agttgtctaa tataatagtg
     3541 aataatgatg aactaaaaaa gaaatatcaa gaagaggtta cagctgttgg agttgcaact
     3601 tcaccaaatc cctttgctat tgtagctaca atagcagcat ataatgaaag tgaagactgg
     3661 cttaatgaat taaatgatta cttagataat aatatacaat ttattgatga atacttaaaa
     3721 gaaaacctac caaaagtaaa acttgtttat ccagaaggaa catatctagc atggttagat
     3781 tttactgctt atggattaaa tgaagtagaa cttgaagatt taatgtttaa aaaagctaat
     3841 gtattatttg atgaaggtta tatatttgga aaagaaggaa taggatttga aagaataaat
     3901 gtagcttgtc cacagtcttt attaaaagaa tgtatggaca gattaaagac tatatttgaa
     3961 aatttataaa gtataggtaa aaatttataa agtgtttaaa aataaaaaaa taataaactt
     4021 gacaaatttt aactaatcac ttatcatact attatgaata aatagaaaac ataagagagc
     4081 taaattttat tgaattaaac tcttaaagac tttgaaagga agagtagtta tagaatgtaa
     4141 ttaaagcgag ttggtgatgg tgtgagacca atatgaagtc tatagtgaag aacatcctgg
     4201 agttgctaac cgaaatttta aaagagtagg cttagtcgga accaaaccgt tatcatattg
     4261 gggtgtatcg agtaatatct gtacattgtg agctgggttt gaatttattt caaactaatc
     4321 agggtggtac cgcggaaaag caacctttcg tcccttttat atagggatga agggtttttt
     4381 taatggaaaa atatataaat atagggagtg aatgatatga gcacaataat accaaaggat
     4441 tataaaagta gtttgaacgt aattgataca caaatagcta taaaaaaatt aaaagatttt
     4501 tttgaaatga gactaagtaa tgaattaaat cttataagag tttcttctcc gttatttgta
     4561 ttgccagaaa caggtgcaaa tgataattta aatggagaaa aggctgtaag ctttgatata
     4621 ccatttatga ataaaaatgc agaaattgtt caatcacttg caaaatggaa aagattagcc
     4681 cttaaaaaat atgggtttga agttggtagt ggtttatata cagatatgaa tgcaataaga
     4741 aaaaatgaag aattagacaa tatacattct ctttatgtag accaatggga ttgggaattg
     4801 gttatagata aaagtagtag aaatgaaaag actctaaaag atgttgtaaa aagattgtat
     4861 ggagtattta aagatactga aatttttgta tgtagtatgt atgaaggtat aaaagaaata
     4921 ttaccagaag aaataacttt tataacatca caagagttag aagatatgta tccagaatta
     4981 tcaccaaagg aaagagaaga taaaattgta aaagagaaga aagcagtatt tataactcaa
     5041 ataggaaaaa cattaatatc aggagaaaaa catgatggaa gaagtcctga ttatgatgat
     5101 tgggaattga atggggatat attattttac aatcctgttt tagattcagc attagaatta
     5161 tcttcaatgg gaattagagt tgatgaagaa tctcttgata gacaattgaa acttgctgga
     5221 tgtgaagaga gaaaagagtt tgactatcat aagatgcttt taaatggcga acttccgtat
     5281 acaattggtg gaggaattgg tcagtcaaga atatgtatgt atttcttaca aaaagctcat
     5341 ataggagaag tacaagttgg agtatggcca caggatatga ttcaaaattg tagtagtgca
     5401 ggaattgaat tattataatt taaatataaa ctaattgata aaatttatat ctaatatgtt
     5461 catcaaatta attatatcgg ttataatgta gaataaccta taattataag aggtgataaa
     5521 tatggagttt ttaaaaataa tcattaatat agttttagat attttaaaaa aaattttggt
     5581 cagatttaaa aatgctaaat ttggtttggt ttttgtattt gatttactta aactacctga
     5641 ttttatgaca gataaaagaa tcaatatagt tgataaaata aaggtaattt ctgtattaat
     5701 ttttactata tcctactttg tatctggggt tgatataatt ccagagatga ttgcaggggc
     5761 atttggtttt atagatgatg ctatagtttt aatttggagt ataggtatag tgaatgaaga
     5821 aattaataag tatagagtta tcgcaaaaaa agataaacat tcaaatataa ttgaaaatgt
     5881 agaattttct ataaaagatg aagaagaata acaatttata tatgttattc ttttttttat
     5941 taacaaaaaa tggttaaatt gttgacggag aggtaaaact ctattatcat aataagtgga
     6001 tatccaatat ttaactaagt attcaatgtg caagaatgct taatttaaaa aagttatgga
     6061 gggtaaatat tatgaagaaa aaaataggtt tagtgcccaa actaatcatc ggtatcatag
     6121 tcggtatttt aatcggaagt tatgcaccag aaataatagt acaaatatta gtaacagtta
     6181 gtactttatt ctcagcattc ctaaaatttg ttataccatt tataattata ggttttgtaa
     6241 cagcaggtat agctgattta gcaacaggtg caggaaagtt attggggata actacgggaa
     6301 tagcatatgg ttctacctta atagcaggat tattatcatt tgcagtatct acattaatct
     6361 tcccaaattt tattgatgca agtgttgcaa gtcaaattgg agacccagag gcaggaatgt
     6421 tagctccaat attcacaatt ccattagagc ctatggtaga tgtaacagct gcaatcgtat
     6481 ttgcatttgt aatgggactt ggaatatcag ctttaagaaa tcatggaaaa ggagagactt
     6541 tatttaattt cttccaagaa tttcaaggaa tcgtaacgaa aacgttgtca acagtgatta
     6601 tacctttatt acctttatac atagcaggaa cttttgcaaa tataactata gcaggggaag
     6661 tatggacaat attaggggta ttctggaaag tatacttagt agtaatacct ttacattttg
     6721 tatatatggc atgtcaattc acagcagcag gtgtattttc aggaaaaaat ccagtaagaa
     6781 tgttaaaaaa tcaagtacct ggatatttaa cagctatagg aactcaatct tcagcagcaa
     6841 ctataccagt taatgttggt tgtgcagaaa aaaatggagt aagtaagcaa ataagagaat
     6901 ttgttgtacc tctttgtgca acaatacatt tatcaggtag tatgatatca ataacaggat
     6961 ttacaactgc agttttaatg atgaatggta tgccacatgg aataggtgta ttattccctt
     7021 atattgcaat gttaggtata gcaatggttg cagcaccagg agcaccaggt ggggctataa
     7081 tgagtgcact tcctttccta ccaatggttg gaataccatc agatggtggg cttgcaagtt
     7141 taatgatagc tttatactta actcaagaca gttttggtac agcatgtaat gtatcaggag
     7201 ataatgcaat agcagtagta gtagataaaa taaatgatgg aatgagtaaa aaaggaaata
     7261 aagaagagca aagtgcttag ttttttaata atcaaaaatg agttatctca aatagagata
     7321 gctcattttt tatggtataa aaaattataa aataaagtga tttttttatt taaaaattgc
     7381 tttaagagat gatattaata atttattttc attaggtttt aatatacata ctctaaagaa
     7441 atactcatct agaccttcaa atgaacaaca atctcttatt attatttttt gtggaagcag
     7501 ttgctctcga agagatttag cagtcaattc ctttgtcttg attttacaca atatgaaatt
     7561 acctttagta ttgtatatat ctaagctttt tatattctta agctctttaa gcaggtaatc
     7621 tctttcagtg ttcattaagg aaatagtatt tgatataaaa tctaaatcac taaacatgat
     7681 ttcacccatt ttagaagcta ttatgtttat attccataaa tctagatttt tatttatttc
     7741 atttttgaca ttagtgttag atataagacc atatccaagc cttattccag gcgttgaaaa
     7801 aaattttgat gtacctctta taacgaataa attagaataa tcatcaacta attgagtaca
     7861 agagtatgta tctgtatcag taaactctac ataagtctca tcaatcatta aaaatgagtc
     7921 tgtatttttt aaaatttctc tcacttctac tttagtaaat gcaaatccag ttggattact
     7981 aggattacag attactacca aatcataatc tttagcattg atagttttta taagattttc
     8041 taaatttata tgaaaattat cttcttcttt tgcaaagtat ttttctatag aacaattaat
     8101 tttagataac tctttttcat actctgaata ggctggagag agtaaaagag cctgtttagg
     8161 gttaattgta tgtataaatg atgaaattag ttctgtagct ccactgccaa gtactatatt
     8221 atctatagaa catttgcagt aattggatat agaagttttt aagtctatgt aatcagggtc
     8281 aggatacata gataccatat ctatattgtt tacaatgtat tgtttggcta aacttgatgt
     8341 accaaaagga tttatatttg agctaaaatc cataaattct tcctttgaaa agccatactt
     8401 actagataaa ctatataaat tagctccatg gtttgtgctc ataaaagtac ctccaaaaat
     8461 aaaattatat aatcgataaa aataactgat taatatatgt acatatagat tataatacaa
     8521 aggaatcgtt attccaaatt tttaatgtaa aaagcaaaaa agtatggata agctgttaat
     8581 ttaggtttaa aaatgatgaa aaagatttta attgatattt tggtatgcta taatatataa
     8641 gttaatagaa ggaaagcggg aggatacaaa atgtacaaag ttacaggaaa aatgttgaca
     8701 gaagaacaaa taaaagaaaa agtttatgaa ttaggaaaaa agatagaaga agactttaaa
     8761 ggcgaagatt tactagtggt aggtatactt aaaggagcta gtgtatttgt ttcagattta
     8821 ataagatgta tagatttgga tgtaaatata gattttatga gtgttacaag ttatggaaac
     8881 tcaacagagt cttctggaac agttaaaata ttaaaagatt tagatgtaga tattgaagga
     8941 aagaatgtgt tgattgtaga ggatataata gattctggtt taacattaag taatctagtt
     9001 gcagctttaa aaactagaaa tccaaaatct ttaaaactat gtactctact tgataaacct
     9061 cagagaagaa aagctaatat acctgtagac tatgttggat ttgttataga agacaaattt
     9121 attgttggtt atggaataga ctatgctgaa aagtatagaa atttacctta tataggtata
     9181 gttgaggatg tagaatagat ttaaatgtga atcaaaagca ctaatttcta agggagttag
     9241 tgctttttag ttaattatat aaaattttag taaaggagac atctatatgg atattcagtt
     9301 aaatatatta ttatttttcc aaaatttaag aaatccaatt ttgaacttta tatttcttgt
     9361 ctttacaata agcacagaag caccgttatt aatattaatt acaacaatta tatactggtg
     9421 tataaataaa aaatgtggac agaaaatact gtttgctata attggaaatt ttgttgtaaa
     9481 tttaggcatc aaagagtttg taaaagctcc cagaccaata ggaataaaag gtcttgaatc
     9541 attgagagtt tctactgctg gtggttattc ttttccaagt gctcatactc aaactgcaac
     9601 aacattttgg gttagtatta tgactatatt taaaaagaga tatcttcatt taataggtgc
     9661 agttatggtt ttaggagtag gattgtctag gctgtattta gctgttcatt ggccaataga
     9721 tgtgatagcg ggttggatat taggaatatt ttttacagtt gtatttgtaa aaatatttga
     9781 ctatattgat gataataaaa attatatttt acttgtagtt ttacttatac catttataat
     9841 agtggctata tttctaaata gccctgaata ttttgaaaaa tttggtataa tagtaggatt
     9901 tgtatttgga tatatggttg aggatagatt tgttaaattt aatacagata ataataacaa
     9961 aaaaataaat tttagcaata aaaataaggg gagtaaaaat ataatttttt caagaatatg
    10021 taggtttttg gttgggatag taacaatagg aatgttatat attggcatta agttgtttat
    10081 ttcaatgtta atagtcactt taaatataag tgactctact atgagtataa tttttatgaa
    10141 ttttattaag aatacagtag ttgtttttta tggaatagca ggagctccag cattatttaa
    10201 acttttaaaa ttaaattgat tttaaggtat agagtcaata gtttaaatta aaaaaattaa
    10261 gggtagaaaa gagtgattat ttgaagaagg tagtaataat aggtggtgga ccagcaggga
    10321 tgatagccgc atcaactgca tgtgagaaag gctatgatgt aacattaatt gagaaaaatc
    10381 ataaattagg aaaaaaactt gcaataacag gaaaaggtag atgtaatatt actaacgctt
    10441 gtgaaataga ggaattaata gaaaatgtac ctacaaatgg aaaatttctt tatagtgctt
    10501 tttatacttt tacaaatgat gatgtaataa gtatgtttaa taatcttgga gtaaagacta
    10561 aaacagaaag aggaaaaagg gtttttccag agagtgataa agcatttgat attgtaaatg
    10621 ctcttgaaag acaacttaaa agtaagaaag taaatatatt attaaattcg aaagtagaaa
    10681 aaataatatc taaaaataat aaaattgaaa aagtaatttt aaacgataaa aaagaaatta
    10741 agtgtgattc agttgtagta gcaactggtg gattaagtta tccacttact ggttctactg
    10801 gtgatggata taaatttgca atttcacagg ggcacacaat tatagataca aaaccatctt
    10861 taattggtat agaagtacaa gaaagtttta caaaagattt agaaaaatta tcattgagaa
    10921 atgttgaaat aagggtgttt aatagcaaac aaaagaaagt ttatagtgat tttggagaac
    10981 ttgaatttac taaatttggt ttagatggac ctataatcaa gtctgcaagt tgtagaatga
    11041 aagatacaag gaaagaaaat tacactatat tattagattt aaaacctgca ttagatgagg
    11101 aaaaattgga taaaagagtt caaaaagatt ttcagaagta tacaaataaa aaatttgaaa
    11161 aagcattaga tgatttactt ccaaaaaaac taatacctat aataataaac ttaagtgaaa
    11221 ttaatccaaa tacggttgtt caccaaattt ctagagagca gagaaaaaac ttagtccatt
    11281 tattaaaaaa tttaaagttt acagtaaaga gatacagacc tattgaagaa gctataataa
    11341 cttctggtgg tgtaaaagta aacgaaataa attcaagtac aatggaatca aaattagtag
    11401 aaggtttatt ttttgctgga gaagtaatag atatagatgc atatacaggt ggattcaatt
    11461 tgcaaatagc tttttctact ggatatttgg caggttttaa ttgctaaaaa ttacctgaat
    11521 taaaaatgtt taaaatcaca taatattatc agcaacacaa aataaattaa acagaaggaa
    11581 ggtaatcaac atggcaagta acaataacaa caacagaaca gtagtaccag gagcaaaaga
    11641 ggctttaaac caaatgaaat tagaaatagc taatgaactt ggtatgagta attatcaaca
    11701 agttgataaa ggaaacctta ctgcaagaga aaatggttat gtaggtggat acatgactaa
    11761 aaaattagtt gaaatggctg aacaacaaat ggctggaaaa agcaacagat agttataaat
    11821 aattatttat aactattggc tattagttta gctagagcta tctcaaatat agaaagtaat
    11881 ttctaaattg agatagcttt tttttattaa attattaaat agatatattt ttatgtaata
    11941 aattttatgt atttggataa aataatacaa atgaatatgt tacataagga gtgatgtaaa
    12001 atgagtagtg taaattttga ggatttaaaa aacaaattta ttaatagtga cttagatgaa
    12061 aaaataagaa tatatacaac aacagaaggt ttatcagtag aacaatttaa agagctacta
    12121 aaatattatc caatacaaca tttatctaaa ctagaaaaag cacttggata gcagtattta
    12181 atccaaaatt aaaaaggcaa tttatttaat attaaaaata ttttttatta aataaattgc
    12241 ctttttataa taggtttaaa ttattatact tttgatatat aattgtagat tctctcattt
    12301 ccaagttcaa tagacccagt attaataaga gtaactattc cttctatatc tctagcagtt
    12361 aagtatttat atggaattaa taaattataa ccagtattac ttagtaagtt ttttaattgg
    12421 gttatttgag agatattgaa acattttgca gttttagtag atttttttct taagttagat
    12481 aaattttcat tttgaatatt tattaaagtt tctatatagt ttctataaga tatcttaaag
    12541 tttgtctcat tgactatttt ttcattaagt atactactga taaaatcttg agacaatata
    12601 ttatctgcaa atttatctgc tgttaatttt ccagatgtta tgcctttttg atattttcta
    12661 agaatttttt tagtatagca gtatatttta tttctcgttt ctaaatcgag ttgttctatt
    12721 tgcacaaaac cacttccttc taaattataa aaatatttat taaaaatcta tacttaacta
    12781 atcaagcttc aataacttat ttacaataaa tactaaataa taaaatattt gttatttttt
    12841 attattacca agtataaaaa atattataat tgtaaaactt acaaatatat gtaaaatatt
    12901 taggagtgat gaaaatgtta aatgggataa taaagctttt ttattctgaa attgtaaaaa
    12961 caaagattaa taacttagat tcaagtggat attctttaag agatgaatgt aaagaaatca
    13021 gaaatataac tctaaagata gatagtgtat tttttaagct tttaaagtta gcttttggtt
    13081 caatattgct tatggtagct tttaatttaa acatggtttt aggtatagga gtgtttttag
    13141 ttggtgtagt ttatatacta tataaaagaa atatggaaga acaagtgaaa gagcatataa
    13201 ataatgtgaa aaataatatc gacatctcta aaataacttt tatggatgaa aaagggagaa
    13261 caggtctaaa tgcattaatt acattacttc ttataggatt attgacagga tttaactgga
    13321 tattagttgc tagttttgtc gttgtttttg tttttactat aaagaatgta tgttaaaata
    13381 ctaataaata gtttaatata tagggggaga aattgagtat gactaatggc agttataaaa
    13441 tgaagaattt agtggaaata tccgctatgg tgactgaatc tacagatttt tttaatataa
    13501 aagatgacat aatagaaaaa atgttagaag ttgttcaccc tacaaaggca tgtgtgaatc
    13561 tattttataa aaatgattct aaatatgcct acttagtatg ttcgcaaacc ttagaatatg
    13621 tacctcaaat ttttccaata aatagtctta aaggtgcaaa aattgatttt gacacatatc
    13681 cagagtatat acatgaggct gtaaatgaaa aaaagataat atatgtggaa aatgtatttg
    13741 aagattctag agcagaaggt gaacgagatt tagcaaaagc tgaaggttat attgggagaa
    13801 tagtttttcc ttttataata aataacgtag ttgttggatt tatgacttgt tttttaaaag
    13861 aggatgatta tataacagaa caagatatag attttatatc atctgttgca tcactcttaa
    13921 gtttatcaat agaaataact aataaaaata ataatgtaca aattttaatt gataaactaa
    13981 gaggctcaat aagtgccata aatgaagcta ctaaaaaact ataccttaat aagagtattg
    14041 atggcttttt ggaaaattta agtaaacaag cgtgtaatat tactaatagt aaagaagctt
    14101 taataattat aaatagtcat cataataagg atgctatagt tagttgttat aatgttgagc
    14161 aaaaaaagaa aacaaattta tatcctatga ttgatatgtt tttaaagaaa gagtcattgg
    14221 gggggtatct aaataattta aaaccatgct caaaaaaggg aataaatttg gaaagttata
    14281 tatattacaa acttcaggat aaaaatgata ctataggatg tatagtttgt gcaaatagca
    14341 aaaactatac tagtgatgat ttaaatattc ttagtatatt gtccaaacaa gtttctgttg
    14401 gtatgcaatt atatgaacat aatgaaaagg aagtaaaaca caaagttctt gaaaacgagt
    14461 tgaacatatt aaataaacaa caacagctta ttatggataa aggtaaaatg gattgtaatg
    14521 atgagaaaga actttatttt taccataaac cagcaagggt agtaggtgga gatttttatt
    14581 atgctacaaa agtggatgac gaacatatag tttatattgt tgctgatgta atgggacatg
    14641 gaatggtatc aaattatgtt gttgcaatga taaaaggtgc ttttaaagta ttatgcaatc
    14701 aatacaatac accaagagaa ataatgacaa atttaaatag gatgttatat gatgaatttg
    14761 acaagatggg agtatttaca acttgtttga taggtattat agatactaaa gaaaatatga
    14821 ttactgtgtc taatgcaggt cactatagtc ctgtagttgt aaagaaagat ggcacaatag
    14881 aaaataattt aaattgtaaa aagggcatac ctattggaat aatggaagat gctacttatg
    14941 aaaataatac tctttcaatg gaagattatg caatggtatg tatgtacact gatggaattt
    15001 tagagataaa aaattcatca aaagaagagt atggtataaa ccgattagaa aactttctaa
    15061 aagaaaattt tagattaaat caacagttaa tagtagaaaa tctaaaattg gatttaaaga
    15121 acttctcatc aaaagataat tatgatgatg atattttaat agttatgtta aaggataggt
    15181 agattgatta tggattcaaa cgataaaaaa ggacttaaaa gtccagagat tataataata
    15241 aatggaagtc caagaattaa taaaaattgc agttttatag caaatgaagt aaaagctatg
    15301 ctagaagagt gtggattaag ttgtaaaata tttaatatct acaatatgga tattgaatat
    15361 tgcacagcat gtggattttg tgaaaaaaca gggtattgca agtttaatga tgatatgaag
    15421 ccattatata aaatgtttga tgattcaaaa ggaactatag taataagtcc tgtacatttt
    15481 gattcaatat cagctaaatt aaagactgtt gttgatagaa ctcaagctat atatgcaagt
    15541 aaatatgtat taaataaacc ttcaattgat agaagtaaga aacggatagg aatgtatatt
    15601 gcagtggggg ggagtaaatc atatgattca cagtttaaag gtggacagat tgtgatggat
    15661 tttttcttta agagtataaa tactaaactt ttacacaata tatatttaaa caatacagat
    15721 gatgtatctt atatagataa tcaagaattt aaagatataa tatataaaaa tttaaagata
    15781 tatattgatt caatataatt tgattttgta ggtaatatta tgtataattg tattgcaata
    15841 atttaaataa ttaaaaggag ggaatttcaa tgaaattacc aatagcatta tctaataaac
    15901 atgttcattt gagtcaagct gatatagacg tattatttgg ggcaggtcat gagttaacta
    15961 aatttaaacc attatctcaa ccagggcaat atgcctgtga agaaaaagtg gatttagtag
    16021 gaccaaaagg aactttaaaa ggagttagag tactaggacc agctagagct catactcaag
    16081 ttgaaatatc tttagcagat ggtttcccat taggagtaaa agcaccaata aaagaatctg
    16141 gtgatatagc tggaactcca ggtgttaaat tagtaggacc tgctggagaa gttgaaatga
    16201 aagaaggagt tatagttgct tctagacata tacatatgag caatgaagat gctgctaaat
    16261 ttggtgtaac tgataagcaa atagtaaaag ttaaaacatc tggaccaaga gcattagtat
    16321 ttgaaaatgt attagtaaga gctagtgaca gctttaatct agaaatgcat gttgatatgg
    16381 aagagggaaa tgcagcaggc gttaaaaatg gagatttagt agaattaata gttgattaat
    16441 attataaata gactttatgc tagtagatat tttatttgct agcatatttt taaatgtaat
    16501 aaatttctat aggataaatt taatcgatta gattaattta taaaataaag gggtattata
    16561 aaaaatgtaa taaaaactta caaaaatatg atgtaat
//
LOCUS       DAMJVK010000091        16252 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.094, whole genome shotgun sequence.
ACCESSION   DAMJVK010000091 DAMJVK010000000
VERSION     DAMJVK010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16252
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.094"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            <1..502
                     /locus_tag="PC414_002940"
     CDS             <1..502
                     /locus_tag="PC414_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2465322.1"
                     /translation="KKSLNKLAKLQRELSRKTIGSLNRNKARLKVARLQEHIANQRND
                     FLQKLSTKLIKENDIICIEDLQVKNMIRNRKLSRSISDVSWSEFIRQLEYKANWHGRQ
                     IVKVGKFFASSQICNKCGYKNEEVKDLNIREWIYPSCNETHDRDINASINILKEGLRL
                     ITIQNK"
     gene            934..1188
                     /locus_tag="PC414_002941"
     CDS             934..1188
                     /locus_tag="PC414_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465323.1"
                     /translation="MENKDIKLTICIFAFVIGIIVLFCCIGLGQFNMDCIIKQNGGSI
                     ATEQYNIYLEQSISQYRNFGSILVLLGGIGILFDKSVRKY"
     gene            1224..1547
                     /locus_tag="PC414_002942"
     CDS             1224..1547
                     /locus_tag="PC414_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465324.1"
                     /translation="MARDVFSVKMTPLEAQKMVEKNLEADLVSVDIYNLENNKSIIVT
                     IFEKYYARSNSDAGLVVICENTTGKTLVKITSTGSAIGLFQIDWGTGNNLIKRVKKIL
                     IDYII"
     gene            1821..1973
                     /locus_tag="PC414_002943"
     CDS             1821..1973
                     /locus_tag="PC414_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465325.1"
                     /translation="MDNFLFNVLASVTASYIVYLISKLIKKVKSHSTAKSDLKIDINF
                     KYHRKK"
     gene            2167..3090
                     /locus_tag="PC414_002944"
     CDS             2167..3090
                     /locus_tag="PC414_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HDF2465326.1"
                     /translation="MVGAFVLPNYAIILSEMSNLWGNFIMWILVPIITIVLLIIAVSS
                     MQYILVMIAFLLIIYSFIEKKIVMGLVSVLFFTYSIYLCATWEDKSLIADNKVETVRA
                     QRETVEREKEMERRRIQEEVDKERYIEKHGMEISENDLKVKLEALVPQEYKGKKYELK
                     VGKFKRYSMYFDLTVQNEKFSNSEECKKFVKEIANDLKKIKISKAYFKFHSKDDGGIY
                     NSVYIDYFRNIQNNVDNVENLEFNEFELKTEEEEKREQEKIEQEKNSYNNYIQNRVVD
                     PLDRIKKLKELLDSGAITQEEYNKKKKELLE"
     repeat_region   3357..4107
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=3357..3385
                     /rpt_unit_seq="gttttagattaactatatggaatgtaaat"
     gene            4298..4450
                     /locus_tag="PC414_002945"
     CDS             4298..4450
                     /locus_tag="PC414_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465327.1"
                     /translation="MKNCRKDKRGFSVLLDKEKLDKFDEVLEEKNLTKKEWLEEKIDE
                     ELEQKE"
     gene            4570..5383
                     /locus_tag="PC414_002946"
                     /pseudo
     CDS             4570..5383
                     /locus_tag="PC414_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894376.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxin Bro"
     gene            5435..5596
                     /locus_tag="PC414_002947"
     CDS             5435..5596
                     /locus_tag="PC414_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465328.1"
                     /translation="MCENLLDNMYIEKRKEEYRIKLLKIRETDIDIYNKLESIVYKLS
                     EKKLEKNNK"
     gene            complement(5924..6388)
                     /locus_tag="PC414_002948"
     CDS             complement(5924..6388)
                     /locus_tag="PC414_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465329.1"
                     /translation="MDFGEVKSELSDKDLKDNEEALNKYKKYYLKVYKYYSEMDSIKS
                     IQDIEIEQSRLKGQLQKNSNPIFALYISIMAVVFSTTINLTFDLSDITNYFIRMIMTL
                     LFIVLLIFLVNKGILPIAIPYRNNIQFYTTALDVLEDMKNKILRERKYKKRR"
     gene            complement(6524..6841)
                     /locus_tag="PC414_002949"
     CDS             complement(6524..6841)
                     /locus_tag="PC414_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465330.1"
                     /translation="MDFGNINLILIGIIVIIGTTIIYLIKPKTAFCSKKYFNKLESIY
                     GNIDKKKTVKLEVLYRYVTGLEYIAIGLFTRRLDITIIAIILVAIITTALYYLIRKKY
                     ITI"
     gene            complement(7124..7420)
                     /locus_tag="PC414_002950"
     CDS             complement(7124..7420)
                     /locus_tag="PC414_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465331.1"
                     /translation="MYFKTYILKREVNNKQLEITRSFDLDNLICTATEILKDNEIDLI
                     ITDEVNNVVWRNEKSTPPARECLVIAIEVYWWYTSIFLLYHINYVIKKNYLNRF"
     gene            complement(7439..7852)
                     /locus_tag="PC414_002951"
     CDS             complement(7439..7852)
                     /locus_tag="PC414_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465332.1"
                     /translation="MKVKSKRSFIVGIIVCVLCCASLVIYCILKDKRFLISSFLLIVI
                     AIFNFCNAFSRKGIVEELHDSTDERDLYLTMKTSHILVKIMNYTLFTFTFLFIIAYSA
                     WKNQSLLVIAITLCVIEIFLFVAYLLINIFLEKKE"
     gene            complement(7836..8069)
                     /locus_tag="PC414_002952"
     CDS             complement(7836..8069)
                     /locus_tag="PC414_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2465333.1"
                     /translation="MKTKVREFRTNMGLTQQQLADLVHVSNRTIISIEKEQYSPSLML
                     AYRMAQVFGTTIEELCCLKENKEMEDKKHESKE"
     gene            8288..8662
                     /locus_tag="PC414_002953"
     CDS             8288..8662
                     /locus_tag="PC414_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2465334.1"
                     /translation="MKISKLPEAELKVMRYICEANKVLTSRELVAAMEEKYEWNESTT
                     FTVLKRLERREFLSTEKIGKLTHYNILVKEKKYLRFETKEFLKNIHKNSISSLMSALH
                     GEDDEVDEDKLSELEECFKNLK"
     gene            9274..9966
                     /locus_tag="PC414_002954"
     CDS             9274..9966
                     /locus_tag="PC414_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2465335.1"
                     /translation="MYNILVVDDDKEIVDSIEIYLKSEGFKIYKAYDGMKALEIITEN
                     DIHLILMDIMMPKLDGIKATVKIREERNIPIILISAKSEDTDKIIGLNIGADDYITKP
                     FNLLELIARVKSNLRRYVTLGNYEDTCKEILKSGALELNTLTKEVKIDGENIKMTPIE
                     YKIIELLLENKGRVFSIDEIYEKVWNEESFNSENTVAVHIRRIREKIEINPKEPRFLK
                     VVWGVGYKIEKF"
     gene            10162..12222
                     /locus_tag="PC414_002955"
     CDS             10162..12222
                     /locus_tag="PC414_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2465336.1"
                     /translation="MEKYRVLKLVSLIIISISFPLTILLVTIGLAEPKYNIQKTSTKS
                     GIFDISDDFYDSNIFDEGTMASLLPKFKLSVGGKLTSKEKGKFQQEGKSIDLSSSDSE
                     YDKIMINEQASEEYLSYLKNIKVYVINTYTGVFYTNTSYASLYDFKQKTKSYCNVEII
                     NKIGKSSYIKTINGEKFELNNLSLELNEVDEGFEAYVSFPEEPTIEDGIVYTNFQIFK
                     QATEKVRLFFVASLSLAIICLLSIILYIKIKGEVLKRNNVFLLIYSKIPMEFNVILVS
                     ISIAILTIYALAVSYHIDVIALVSLSLLIVSNNIFFINVQLSYLENKMCIFKTSIIVR
                     VILWLIKTVREVSNAIKKVSLARKVIVIVIISLALIMAISTRSIGMGLLFSICIFLGF
                     TIYITKRLSYLSYVIEGTERIKKGELDYKIKITGNDNFTSLAENINNIGEGLDRAIEE
                     QVKSERMKSDLITNVSHDLKTPLTSIINYVDLIKKEESIQPEYINDYINVLESKSKRL
                     KLLIEDLFEASRVSSGNIELQISKIDLVQLLRQSIGELEEKLSKNNLYLKLNVPNDKV
                     YIWADGRRMYRVFENLLSNIAKYSLEETRVYIDVYDYGENVKVTMKNISSYELNFDPS
                     EIMERFKRADESRNTEGSGLGLAIARDLVALQGGKFYIEIDGDLFKANLEFQKYEEEI
                     TNME"
     gene            complement(12427..12750)
                     /locus_tag="PC414_002956"
     CDS             complement(12427..12750)
                     /locus_tag="PC414_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDF2465337.1"
                     /translation="MNNNYIFFIIIGMYIVTYLPRVLPMLIFSKKEMPDFLKKIMKFI
                     PVAILTSLTAKDVFFNGDSLYLSIANPKIISFMIVLLVAYKFKSIGISIITGVISIYL
                     FGIIL"
     gene            complement(12743..13444)
                     /locus_tag="PC414_002957"
     CDS             complement(12743..13444)
                     /locus_tag="PC414_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDF2465338.1"
                     /translation="MERTQQLKESFIASIPIFLGYIPIGIVGGILLQKSGLSPFQIAL
                     MVTLVFGGSSQFIAASMISTGASVTSIVLTTFIVNLRHFLMSSNLNMYIKNKSPKFIL
                     PFCHTITDETFAVNYEKFTAGNWSDRNAIYLNFFCLISSVLANFIGAFLGESISIDSS
                     ISGFILTSMFIALIVSQIKNKIYIVVFISSIIISVILYALFKSNLVIIVASILASLIG
                     FFIEESYCKKEESYE"
     gene            complement(13722..15107)
                     /locus_tag="PC414_002958"
     CDS             complement(13722..15107)
                     /locus_tag="PC414_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF2465339.1"
                     /translation="MPINSFENYPMNWKRKRPSKGQILYKALAEQLEQDINNGFLLPG
                     TKLPPQRELADFLDVNVSTISRAFKICEKKGLISGVTGSGTFVSYDTRSNLFLMSSNN
                     KITFIEMGTMNPDFTLEEMNTLFKHIIKEIDFKTIFQYGQRDGAKWQKEAIAKLIYKA
                     GLETTSDSLLPASGGQNAIVAILAGLFQHGDRIGVDPLTYPGIKTAAKMLGIQLIPIK
                     QEDNEISEEGLLYACKNENIKGLYIIPDYQNPTTHIMSQNGRKMIANIASKYNLIVIE
                     DAIHSLLNETHLNPVASYLPNQTIYITSLSKIIAPSLRLAYISTPKQYREALSSALYN
                     INLSQSYFLTEIAYRMITSGEADKLINARRKSARRRNKIINQYLSGYNLLGNEECIFR
                     WLILPDGIMAEKFEIQALKEGVQVYASERFAVGKEKPISAIRIAVCATESTEELKAGL
                     SVLKRLLEEKK"
     gene            complement(15215..>16252)
                     /locus_tag="PC414_002959"
     CDS             complement(15215..>16252)
                     /locus_tag="PC414_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HDF2465340.1"
                     /translation="YKHNIDNYIPYDQGITLDIFMYIWILGIIAISFYYLRTNFKFYN
                     YIENFKKEILEIDITSILDEQAKELSINKKLQIYKVKGIYSPAIIGLTRCKIIIPEKK
                     YNNKELRFIFKHELIHYKRKDNLFKLLISIICIIYWFNPILYLLKKYFHELCELSCDE
                     IVIDKCTTSEIKEYAYIILDTIKYQKSLKYSSFVSQFLNKKNIILKKRLDSMFNQDKK
                     KINLGLKFLLTFIIIGSIFSINSNFKQNEDVEYTIGKINYNKNGETYGTHIIDSNGNS
                     IEPELIQTIGRGKKVGYVKKRDLYDCDNQPKTPEEAVLYTRKRNFNIFKKTIPLYDKE
                     GIKIIGRFEIN"
BASE COUNT         6411 a         1780 c         2460 g         5601 t
ORIGIN      
        1 taagaagtca ttaaacaaac ttgctaaatt acaaagagaa ttatcaagaa aaacaatcgg
       61 tagtttaaat agaaataaag caagattaaa agttgcaaga cttcaagaac atatagctaa
      121 tcaaagaaat gattttctac aaaaactatc tactaaatta ataaaagaga acgatattat
      181 ttgcatagaa gatttacaag taaagaatat gattaggaat cgtaaactat ctcgctcaat
      241 ttctgatgtg tcatggtcag agtttattcg tcaattagaa tacaaggcta attggcatgg
      301 tagacaaatt gtaaaggtgg gtaaattctt tgcaagttca caaatatgca ataaatgtgg
      361 gtataaaaac gaggaagtga aggacttaaa tataagagaa tggatttatc ctagttgtaa
      421 tgaaactcac gatagagata taaatgcaag tataaatata ttaaaagaag gattaagact
      481 aataacaatt caaaataaat aacgtatgta agaaccgtag gaactacggg gatagcctgt
      541 ggagaattag taagacatat gttagtatgc aaaattctgt gaagcaggaa acctgtgact
      601 ttagtcatgg gaggttcagg ctagtattac tcattattgt attcaaaagc taaagtggaa
      661 acctagtgaa tatatgaatt tagaagttaa tgagagagca ttagcagccg actcaatact
      721 tattaaaatt gaagatgaag aagaaacaat gaaagaagct gaaagagaga aaaagagagg
      781 tagaagaagg tagaatttaa agttaaaata gttacattgc taatatatga aaatgttaca
      841 aatttggaaa tagatggata tatatgttgt atagtataat gtagataata tatcaccaag
      901 taaaaaacaa caaataaatc taaattatga ggggtggaaa ataaagatat taaacttaca
      961 atatgtatat ttgcttttgt aattggaata atagtgttat tttgctgtat aggtctaggt
     1021 caatttaata tggattgtat tataaaacaa aatggtgggt caatagctac cgaacaatac
     1081 aacatatatt tagaacaatc tatttcacaa tatagaaatt ttggttctat actggtttta
     1141 ctaggaggaa taggaatact gtttgataaa agtgtcagaa aatactaaaa aagtatattt
     1201 ttatttagga tgtgatgaat gatatggcaa gagatgtatt tagtgtaaag atgacaccac
     1261 tagaagcaca gaaaatggta gaaaaaaatt tggaagcaga tttagtttct gttgatattt
     1321 ataacctaga aaataataag tctataatag ttacaatctt tgaaaaatat tatgcaagaa
     1381 gcaatagtga tgcaggtttg gtagtgattt gtgaaaatac tacaggaaaa acacttgtta
     1441 aaattacctc aactggtagt gcaattggac tatttcagat tgattgggga acaggtaata
     1501 atttgattaa aagagtaaaa aaaatactta ttgattatat tatttaatta ttttatgtag
     1561 atattcttag gttggtatgg aatttgtact aacctattaa gtaatcaata aaactaaata
     1621 aacatagaaa gcacttacaa atatgtaggt gctttttatt gaaaaaatag aaaataaaat
     1681 aaaaagtaag atataggaaa aatatgtaag aattatatgt tataataatt gtagcaagaa
     1741 gatgtaatct acaatttata gagtggagtt catgtattaa aaaattatcc tcccaacgat
     1801 aagaagggag gtgaatatac atggataatt ttttatttaa tgtgttagct agtgtaacag
     1861 ctagttacat agtttatttg attagcaagt taatcaaaaa agtaaaaagc cactctaccg
     1921 caaagagtga cttaaagatt gatataaatt ttaaatatca tcgtaaaaaa taactttcag
     1981 ttatagaact tcactctacc gcaaatagat tgtagttctt cttgttttta ttataccaca
     2041 aattagaaaa aatattgcat ataatatttt ttatagtcaa taaaaatatg aaatttttta
     2101 tacacaatga tgatgaatct aactagatga ataaatttaa ttgtaaataa gaaagtactt
     2161 acttaaatgg taggtgcttt tgttttgcca aattatgcta taatattgag tgagatgagt
     2221 aatttgtggg ggaattttat aatgtggata ttagtaccaa taataacaat agtattactt
     2281 ataatagctg tttcttctat gcaatatatt ttagttatga tagctttttt gttgattata
     2341 tattctttta tagaaaagaa aatagttatg ggtcttgtgt cagttctttt tttcacttat
     2401 tcaatttatc tttgtgcaac ttgggaagat aaatccttga tagcagataa taaagttgag
     2461 actgttaggg cacaaagaga aactgtagaa agagaaaaag aaatggaaag aagaagaata
     2521 caagaagaag tagacaaaga aagatatatt gaaaaacatg gaatggaaat atcagaaaat
     2581 gatttaaaag ttaagctaga ggctttagta cctcaagaat ataaggggaa gaaatatgaa
     2641 ttaaaagtag gaaaatttaa aagatatagt atgtattttg atttaactgt acaaaatgaa
     2701 aaatttagta attctgaaga atgtaaaaaa tttgtaaaag aaattgctaa tgatcttaaa
     2761 aaaattaaaa taagcaaggc gtactttaag tttcattcaa aagatgacgg cggtatatat
     2821 aattctgttt atattgatta ttttagaaac atacaaaata atgtagataa tgttgaaaat
     2881 ttagaattca atgagtttga acttaaaacc gaagaagaag agaagcgaga acaagagaaa
     2941 atagaacaag aaaaaaacag ttataataac tacattcaaa acagagtggt tgacccacta
     3001 gatagaataa agaaactaaa agagttgcta gattcaggag caattacaca ggaagaatat
     3061 aataaaaaga aaaaagaatt attagaatag ataatatagt aagcacttac aagtatgtag
     3121 gtgcttttaa atttacaaag tattccattt taattttata gtttagattt tatgatataa
     3181 taaaaatata gaagttttgc agtgtgcgat atttgttaca aagtagggct taatacttga
     3241 aatctaagat gttgagggtg cgtgataagt gttatcaatt gcactattgc ccgctcactg
     3301 caattttaag agtattgtat atatgtaggt attggaaatg ctaagtttat tttggggttt
     3361 tagattaact atatggaatg taaatattac atgggtaaaa attttgatga tttttatgac
     3421 agttttagat taactatatg gaatgtaaat attacattta atttgcattg ctttgatgac
     3481 ttctttgttt tagattaact atatggaatg taaatgcgta caaggttgag aagctccgca
     3541 atctcaacgt cgtgttttag attaactata tggaatgtaa atatttgaaa ggaataaatt
     3601 ttaaatgaat gaaaggatag gttttagatt aactatatgg aatgtaaatc agccaaaaac
     3661 ttcgaatttg aactaaaatg cgtgggtttt agattaacta tatggaatgt aaattctcat
     3721 gactattgaa taaatataat aaacaagcat acgttttaga ttaactatat ggaatgtaaa
     3781 tttagaatca aaagcactta atcctagttc tttcatagtt ttagattaac tatatggaat
     3841 gtaaatagct ttttagcttc gtctactact ttataagcaa agcgttttag attaactata
     3901 tggaatgtaa atgatgaaca ataaaacaat catctaaaga cgaagaaagt tttagattaa
     3961 ctatatggaa tgtaaatgtt cctgttgtgt tttttactaa tcccatttta tatgttttag
     4021 attaactata tggaatgtaa attgctgtga tttcactaat aaaacaatta ataattgtgt
     4081 tttagattaa ctatgtggat tcaaaattaa ataaacaaag aaagcactta caaatatgta
     4141 ggtgctttta ttctgctcaa aattggtcgg ttgggtaaaa tcattagaaa aaattagtaa
     4201 aaacctcttt tctgtaactc gttacaatat tattattaat gtaacgagtt acagaaaaga
     4261 ggtgaataaa atagcaacta aaagtagggc agagtatatg aaaaattgtc gaaaagataa
     4321 aagaggtttt agtgtacttt tagacaaaga aaagttagat aaatttgatg aagtattaga
     4381 ggaaaagaat ctaaccaaga aagaatggct agaagaaaaa atcgacgagg aactggaaca
     4441 aaaggaataa aaaataaggg tcactcccac cgaccaaagt ttgagtaacc cctatgatgt
     4501 atactatcgt atatcaatta taatatatgt cattccttaa aaaaatcaat tattaaggag
     4561 tgtaatatta tgaaaaatga attaatgatg tttgaaggaa aagagattga agtatttgaa
     4621 tttgaaggaa gaattttatt caactcaaaa gatgtagcaa attgtttaga cataaaaaat
     4681 gttaatgaga atataatact tatgaatgaa aagcaagttg taaagttaag aaattcggat
     4741 atcagtaata ctgacatccg aaaacttaat aatgctggag aaaattttct tactgaaagt
     4801 ggagtgtata aattaatctt taaatcaaga aaagaagaag ctgagagatt ccaagactgg
     4861 ataagcgatg aagttttacc aagcataaga caaactggtg catacataac taataatgta
     4921 atcccgaaaa gttgagagaa aaagcaagtg agattgaaaa attacagttg cttataacag
     4981 tacatctatg ttaaaagaat tgctagatga tgcaggcttt gacaataaat caaaactatt
     5041 aacagctaaa acattatata agaaagcaga aattgattta ccaatagaga taaacgaaga
     5101 agaacattat tttgatacaa aacaaatagc atctaaactg aaaatatatt ctaagagtaa
     5161 taaaccagct cagatggctg tttgtgagat tattaaaaag attgatttag aagatggtga
     5221 agttaaaggt gtttgggaaa ttaatggttc ttggactggt actgtaaata aatatacaaa
     5281 gagtgtaata gataaggtga gaacttggat agaggaaaat aatagaccta ccaagattgc
     5341 aggtgagaag aagaattatt atgtatttta caaaattgag taaatttatc agttgtatta
     5401 aataatttag tttagttttg gggggattaa tacaatgtgt gagaatttac ttgataatat
     5461 gtatattgaa aagagaaaag aagaatatag aattaaactt ttgaaaataa gagaaacaga
     5521 tatagatata tataataagt tagaaagtat agtatataaa ctttctgaga aaaaattaga
     5581 gaaaaataat aaatagacat ataaagcact tggatattct actgtttcaa gtgcttgttt
     5641 ggtataaaat ggtataatgg agatagagaa ttatattaac caaaaaatgg agaaaataga
     5701 gaagatgatt tagttgacgc tttatcagta ggagtacctg caggtaagac agaatctcta
     5761 gtacgattag ttggagatgg aataagtgat actcaaaaaa gtttaataaa taaaagagaa
     5821 tttagaaata taactcaaat tggtggaaat ggaaatgaga aagcatttag tgaagtagaa
     5881 aacttagtta aatagattag aaggtagcaa ctagagttgg ttgttacctt ctttttttat
     5941 actttctctc tctaagtatt ttatttttca tatcttctaa tacatctaat gcagtagtat
     6001 aaaattgtat attatttcta taaggtatag ctattggaag aatgcccttg ttaactaaaa
     6061 aaataagaag tacaataaat aaaagtgtca taatcattct aataaaataa ttagtgatat
     6121 ctgataaatc aaatgttaag ttaattgttg tagaaaatac aacagccata atagatatat
     6181 acaaagcaaa aatggggtta ctattttttt gtaactgtcc tttaagccta gattgctcta
     6241 tttctatatc ttgaatactt tttatggaat ccatttcact ataatactta taaactttta
     6301 aataatattt tttatacttg tttaaagctt cttcattatc tttaaggtct ttgtcactta
     6361 actctgattt aacttcccca aaatccataa aatccctcct acaatgttat ataatatact
     6421 ttaatttata gcatatttaa gaacttatat cagcaatttg tcgaacgatt ttagctaaaa
     6481 atagagtata atgataggct tattatttat aagatattat tttttatata gttatatatt
     6541 tctttctaat tagataatat aatgctgttg ttataatggc tactaatatt attgctatta
     6601 ttgtaatatc taatcttctt gtaaataagc ctattgctat atactcaaga cctgttacat
     6661 agcgataaag tacttctagt ttaacagttt ttttcttatc tatatttcca tatattgatt
     6721 ctaatttatt aaaatatttt ttactacaaa aagcagtttt aggtttaatt aaatatatta
     6781 ttgtagttcc tattataact attattccaa taagtataag attgatattt ccaaaatcca
     6841 tagcagatac ctcctattaa atttgctttt cttaatcaaa gtatatcagt aacacaagta
     6901 caaagagtat ctaaaatatt acaaatatta acaggataga taaaactata gtaaaaattg
     6961 taataataaa gcgaacgaaa attatcatac taaaacataa gacatgttat aattgtatta
     7021 gatagaagat taacatacac caaagcactc cttataataa agagtgctta tttttttgaa
     7081 attcatcaat atataaacta tcaagaacct tattcaacac accttaaaat cgatttaaat
     7141 agttcttttt gataacataa tttatatgat ataataaaaa aatagaagtg taccaccaat
     7201 acacttctat agctataact aagcattctc ttgctggggg agtgcttttt tcatttctcc
     7261 aaacaacatt atttacttca tcagtaatta tcaaatcaat ctcattatct ttcaaaatct
     7321 ctgttgcagt acaaattaga ttatctaagt caaaacttcg agttatctct agttgtttgt
     7381 tatttacttc tctttttagt atataagttt taaaatacat ttatatcacc ccttgtattt
     7441 actctttttt ctctaaaaat atattgataa gtaaatatgc aacaaataaa aaaatctcaa
     7501 tgacacatag tgttatagct ataactagaa gtgattgatt tttccatgca ctataagcta
     7561 tgatgaaaag aaatgtaaaa gtaaataatg tataattcat aattttaact aagatatgac
     7621 ttgttttcat tgttaagtac aagtctcttt catctgtact atcgtgtaat tcttcaacaa
     7681 tgccttttct tgaaaaagca ttacaaaaat taaagattgc tattacaatt aaaagaaatg
     7741 atgaaattag gaatctttta tcttttaaaa tgcaataaat aactaaacta gcacaacata
     7801 acacacaaac tataataccc actataaaac ttcgtttact ctttactttc atgtttttta
     7861 tcctccattt ctttattttc ttttaaacaa catagttctt caatcgttgt accaaacact
     7921 tgtgccatac gataggcaag cataagagaa gggctatatt gctctttttc aatagaaata
     7981 attgttctgt ttgatacatg gactaaatca gcgagttgtt gctgtgttag acccatattt
     8041 gttcgaaatt ctcgtacttt tgttttcatt ccataacctc tttctatatg aagctaactt
     8101 catgtgaagt taacttcata atatcctaaa atgagtaaaa tgtcaatctc attagataag
     8161 tattataaaa cctaaaagaa taatatggta aaaattggat ggaattattt accttaattc
     8221 tcttgtttgt aaaactttat tgatatagaa tttaacatat acataaatcg gcaaaggggc
     8281 gcgaattatg aaaattagta aattaccaga agcggaatta aaagttatga gatatatttg
     8341 tgaagctaac aaggtattaa cttctaggga attagttgct gcaatggaag aaaaatatga
     8401 gtggaatgaa tcaactacat ttacagtttt gaaaagatta gagcgaagag agtttttaag
     8461 tacagagaaa ataggcaaac ttacacatta taatatacta gtaaaagaga aaaaatatct
     8521 aaggtttgaa acaaaggaat ttttgaagaa catacataaa aattcaattt caagtttaat
     8581 gtcagcgtta catggtgaag acgacgaagt ggatgaagat aaattaagtg aactcgaaga
     8641 atgttttaag aatttgaaat aagaaaatga caaattgata cataataaat aaaatgaagg
     8701 tatgaaatta taactttttc taccttcatt ttgtttatta ttctgaacat gttaagaaaa
     8761 tgaaataagt ttgtatttta gttatataaa ttttaataga atatgtacag tatatagtag
     8821 atagaatgta taaaaataga atttaaataa gtagctatta ttgcagtgaa atgaatgttt
     8881 ctaagtatat ttgggtggaa gaatggtgga agaaaattag tgaaatgtaa ttcttccacc
     8941 attcttccac ctagaaaata gaaactgttt aataagaatt aatatacatt aattcagctg
     9001 aatgcaatca aaatacttaa atgaaagtaa tctgaaaaca acataattca aatcaattta
     9061 ttcaaataaa cctcaattta tcttaaatct taagaaatat ttaagaatta ggataatatt
     9121 ttcttaagat ttatatttta ttatatagtt ataagggaaa gttacaaaaa tgtaagccaa
     9181 agtaatataa caatttagtc attgttgaga aagggagtga aagatattgt ataatataga
     9241 taagtattat tatacaatag aggaagtgaa aatatgtaca atatattagt agtagacgat
     9301 gataaagaaa ttgttgattc gatagaaata tatcttaaat cagaaggatt taaaatatat
     9361 aaagcatatg atggaatgaa agctttggaa ataataacag agaatgacat tcatctaata
     9421 ctaatggata taatgatgcc taaattagat ggaatcaaag caactgtaaa aatacgagaa
     9481 gaaagaaata ttccaataat tttaatttca gcaaaaagtg aagatacaga taaaataata
     9541 ggattaaata ttggagctga tgattatata acaaagccat ttaatctatt agagcttata
     9601 gctagggtta aatcaaactt aagacgatat gtaactttag gtaattatga agatacatgt
     9661 aaagaaattc taaaaagtgg agctttggag ttaaatacac taacaaaaga agttaaaatt
     9721 gatggtgaaa acataaaaat gactccaatt gagtataaaa taattgaact tcttttagaa
     9781 aataaaggac gagttttctc aatagatgag atatacgaaa aagtatggaa tgaggaaagt
     9841 tttaattcag aaaatacagt agcagtacat atcagacgaa taagagaaaa gattgaaata
     9901 aatccaaaag aaccaagatt tttaaaggtg gtgtggggag ttggttataa gattgaaaaa
     9961 ttttagtgga aaaattataa aagaaaagag tttaatattt aattgcattt tgattttagt
    10021 catttttaca tttacacaaa tgttaggaat tttaatatgt tttaattttt tctctgagag
    10081 tttaatttac tctatgggga ttatgctttt cttaaacttt attttatata aaatactgac
    10141 aatgtagatg aggtgggcaa cttggagaaa tatagagtat taaagttagt ttcattaatc
    10201 ataatatcta tctcatttcc attaactata ttgttggtga caataggact tgctgagcct
    10261 aaatacaata tacaaaaaac ttctacaaag agtgggattt ttgatatatc tgatgatttt
    10321 tatgatagta atatatttga cgaaggaaca atggcaagtc tattacccaa gtttaagcta
    10381 tctgtaggtg gtaaattaac tagtaaagaa aaaggtaaat tccaacaaga aggtaaaagt
    10441 atagatttaa gtagtagtga ttctgagtat gataaaatca tgattaatga acaagcttct
    10501 gaagaatatt taagttatct aaaaaatata aaagtgtatg ttattaatac atacacagga
    10561 gtattttata caaatacgtc atatgctagt ttatatgatt ttaaacaaaa aactaagagc
    10621 tattgtaatg tagagataat aaataagata ggaaagagct catatattaa aacaataaat
    10681 ggagaaaaat ttgaactgaa taatctgagt ctggaactaa atgaagtaga tgaaggtttt
    10741 gaggcatatg tatcttttcc agaagagcca actatagaag atggaatagt atacactaat
    10801 tttcaaatat ttaaacaagc tacagaaaaa gtaagattat tttttgtagc tagtttaagt
    10861 ttagcaatta tatgtttatt atctataatt ttatatatca aaataaaagg tgaagtttta
    10921 aaaagaaata atgtattttt actaatatac agtaagatac ctatggaatt taatgtaata
    10981 ttagtatcta tttcaattgc aattttaact atatatgctc ttgctgtatc ttatcatatt
    11041 gatgtaattg cattagtttc tttatcttta ctgatagtat caaataatat attttttata
    11101 aatgtgcaat tgagctattt agaaaataaa atgtgtatat ttaagacaag tattatagta
    11161 agagtgattt tatggttaat aaaaactgta agggaggtca gtaacgctat aaagaaagta
    11221 tctttagcaa ggaaagttat tgttatagtg ataatatctt tagctttaat catggcaatt
    11281 agcactcgaa gtataggtat gggattactg ttttctatat gtattttttt aggatttaca
    11341 atatatataa ctaaaagact ttcatatctt agttacgtaa tagaaggtac agaaagaata
    11401 aaaaagggag aattagatta taagataaaa attactggga atgataattt tacgtcttta
    11461 gctgaaaata taaataatat tggagaaggt cttgatagag ctatagaaga gcaagtaaaa
    11521 agtgaaagaa tgaagtctga ccttataaca aatgtatctc atgatttgaa aactccactt
    11581 acttctataa taaattatgt agatttaatt aaaaaagaag aatcaataca accagagtat
    11641 ataaatgatt atataaatgt acttgagtca aagtcaaaaa gactaaaatt attgatagaa
    11701 gatttatttg aagcgagtag agtaagtagt ggcaatatag aattacaaat tagcaagatt
    11761 gatttagtgc aactattaag acaaagtata ggtgaacttg aagaaaaatt atctaaaaat
    11821 aatttatatt taaaattaaa tgttccaaat gataaggtgt atatatgggc agatggaaga
    11881 agaatgtata gagtatttga aaatctttta tctaatatag caaaatattc cttagaagaa
    11941 actagagtct atattgatgt atatgactat ggtgaaaatg taaaggtaac tatgaaaaat
    12001 atatcttctt atgaattaaa ttttgaccca agtgagatta tggaaagatt taaaagagct
    12061 gatgaatcaa ggaatactga aggaagtgga ttagggcttg ctattgcaag agatttagta
    12121 gctttacaag gtggaaaatt ttatatagaa atagatggag atttatttaa agcaaattta
    12181 gagtttcaaa aatatgaaga agaaataaca aatatggaat gagatagtat ttaagtatat
    12241 atattttttt actgtcgaaa gcttttatta taatatatta gaatatttac agtagagaat
    12301 gaagagtaaa aacagagtaa ctttaagtca ctctgttttt tatttaaaat caataatata
    12361 acaacttaaa ttaaaattgt acacatagtt ttaaaataac ttatacaata aaacattatc
    12421 caaatatcac aagataattc caaataaata tatagatatc actcctgtaa taatagaaat
    12481 accaatagat ttaaacttat atgcaacaag aagtacaatc ataaaagata taattttagg
    12541 gttagctata gataaatata gactatctcc attaaaaaat acatctttag cagttaagga
    12601 agtaagtatt gctacaggta taaatttcat gatttttttc aaaaaatcag gcatctcttt
    12661 ttttgaaaaa attaacatag gaagcactct tggtagatat gtaacaatgt acattccaat
    12721 tataataaaa aatatataat tattattcat aagattcctc ctttttacag taagattctt
    12781 ctataaagaa accaattaaa gatgctaaaa ttgatgcaac tattattact aaattgcttt
    12841 tgaataatgc atataaaata acagaaataa tgattgatga tataaatact acaatataaa
    12901 tcttattttt tatttgagaa actattaaag ctataaacat agaagtcaat atgaagccag
    12961 aaatagaact gtctatagat atactttctc ctaaaaatgc tccaataaaa ttagctaaaa
    13021 ctgatgaaat taagcagaag aaattaagat aaatagcatt tctatcagac caatttccag
    13081 cagtaaattt ttcataatta actgcaaaag tttcatctgt tatagtatga caaaatggca
    13141 atataaattt tggactttta ttttttatgt acatgttcaa attagaactc attagaaaat
    13201 gtcttaagtt aactataaat gtagtcaaaa caatagatgt tacacttgca ccagtagaaa
    13261 tcattgaagc tgcaatgaat tgagaacttc ctccaaatac taaagttacc attagtgcta
    13321 tttgaaaagg tgaaagacca gatttttgta atagtattcc tcctactata cctataggta
    13381 tataacctaa gaatatagga attgaagcaa taaagctttc ttttaattgt tgagttcttt
    13441 ccacaataaa tcactccttt tgtattaaat gtattaaagt attcgtaaaa cagaaaaata
    13501 gattaattgt ataattgcaa ttataattaa aaataaattt ggattaatat ataaaatact
    13561 atacaacaaa tagataatct taaacggtta ttaaatttta tctatttgtt attatgatgt
    13621 aataatacat attaatctct ataagttaat tactatctgg tgtaatttta ttttaacaac
    13681 aaaaaaaaat acacacaatt aaaatattgt gtgcaataca attatttttt ctcttctaat
    13741 agtcttttta atacgctcaa accagcttta agttcttctg tactttctgt agcacataca
    13801 gctattctta ttgcagaaat tggtttttct tttccaacag caaaacgttc agaagcatag
    13861 acctgtacac cttctttaag tgcttgtatc tcaaattttt ctgccattat tccatcaggt
    13921 agaatcaacc atctaaatat acattcttca tttcccaaaa gattatatcc agataaatat
    13981 tgattaataa tcttgtttct tcttcttgca gatttacggc gtgcattaat taatttatcg
    14041 gcttcacctg atgtaatcat gcgataagca atttctgtta aaaaataaga ttgagataga
    14101 ttaatgttgt ataatgcgct tgataatgcc tctcgatact gtttaggtgt agagatatat
    14161 gctagccgta aactaggtgc aataattttt gataaactcg taatataaat tgtttgattg
    14221 ggcagatagg aagccactgg atttaaatgt gtttcattaa gtaaactatg aatagcatcc
    14281 tcaataacaa ttaaattgta tttagatgca atatttgcaa tcatttttcg tccattttgt
    14341 gacattatat gtgtagttgg attttgataa tctggtatta tgtatagacc tttaatattt
    14401 tcatttttac aagcatataa tagtccttct tcactaatct cattatcttc ttgtttaatt
    14461 ggaattagtt gaatcccaag cattttagct gctgtcttta ttccaggata agttaatggg
    14521 tctacaccta ttctatctcc atgttgaaac aaaccagcta aaatagcaac aattgcattt
    14581 tgtccaccac ttgcaggaag taagctatct gatgtagttt caagcccagc tttataaatt
    14641 aatttggcaa tagcttcttt ttgccattta gcaccatctc tttgtccata ttggaatatg
    14701 gttttaaaat ctatttcctt aattatgtgt ttaaacaaag tattcatttc ttctagtgtg
    14761 aaatctggat tcattgtgcc catttcaata aatgtaattt tattattact ggacatcaag
    14821 aatagatttg aacgtgtatc ataagataca aaagttccac ttcctgtaac tccacttatt
    14881 agaccttttt tttcacaaat tttaaaagct ctagagatag tactcacatt aacatctaag
    14941 aaatctgcca attcacgttg gggtggtaat ttagtacctg gtaataaaaa tccattgttg
    15001 atatcttgtt ctagttgttc tgcaagcgcc ttatataata tttgcccttt tgaaggtctc
    15061 tttcgtttcc agttcatagg ataattttca aaagaattaa ttggcataag ttttcacctc
    15121 acattattaa tttgattttt tctttatcta aaacaataaa ttctaaagat acaaataaaa
    15181 actgatagga aataattcta tcagtttttg tcttttaatt gatttcaaat cttcctataa
    15241 tttttatacc ttctttatca taaagaggga ttgttttttt aaatatatta aaatttcttt
    15301 ttcttgtata taaaacagct tcttctggtg tctttggttg attatcacaa tcgtacaaat
    15361 ctcttttttt tacgtagccg acttttttac ctctacctat tgtttgtata agttctggtt
    15421 caatggaatt tccatttgag tctattatat gtgtaccata ggtttctcca tttttattat
    15481 aattaatctt accaatagta tattctacat cttcattttg tttaaaattg gagttgatag
    15541 aaaatattga ccctattatt ataaaggtta ataaaaactt aagacctaaa ttaattttct
    15601 ttttatcttg attaaacata gaatctaacc tcttttttaa aataatattt tttttattta
    15661 gaaactgaga tacgaatgat gaatacttca aagatttttg atactttatt gtatctaata
    15721 ttatatatgc atactcttta atttcactcg tggtacactt gtcaatcaca atttcatcac
    15781 aagataattc acataattca tgaaaatatt tttttaataa gtataaaatt ggattgaacc
    15841 aatatatgat acaaattata ctaattagaa gcttaaataa gttatctttt cttttataat
    15901 gaattaattc atgtttaaag atgaatctta actctttatt attgtacttt ttttctggaa
    15961 ttattatttt acagcgtgtt aaacctataa tggcaggaga ataaatacct ttaaccttat
    16021 atatctgaag ttttttattt atacttagtt cttttgcttg ttcatctaag atactagtaa
    16081 tatctatttc taaaatttct tttttaaaat tttctatgta attataaaat ttaaaatttg
    16141 ttcttaaata gtaaaaagag attgctataa ttcctaatat ccagatgtac ataaatatat
    16201 ctaaagtaat gccctggtca tatggtatat aattatcgat attatgctta ta
//
LOCUS       DAMJVK010000092        15978 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.095, whole genome shotgun sequence.
ACCESSION   DAMJVK010000092 DAMJVK010000000
VERSION     DAMJVK010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15978
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.095"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            248..397
                     /gene="rpmG"
                     /locus_tag="PC414_002960"
     CDS             248..397
                     /gene="rpmG"
                     /locus_tag="PC414_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004402360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDF2465341.1"
                     /translation="MRVKVTLACTECKQRNYNTTKNKKNNPDRIELQKYCRFCKKHTT
                     HKETK"
     gene            423..644
                     /gene="secE"
                     /locus_tag="PC414_002961"
     CDS             423..644
                     /gene="secE"
                     /locus_tag="PC414_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HDF2465342.1"
                     /translation="MAAQKNDGAKTKKRFSLFGYLKETKQELKRVTWPTKKELFKNTS
                     IVLTVVISCTILVWGIDTILSGALALLLK"
     gene            664..1206
                     /gene="nusG"
                     /locus_tag="PC414_002962"
     CDS             664..1206
                     /gene="nusG"
                     /locus_tag="PC414_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination factor
                     NusG"
                     /protein_id="HDF2465343.1"
                     /translation="MSELQEASWYVVHTYSGHENKVKATIEKAVKTRGMEDCIRQVVV
                     PTEEVVETTKTGKEKTRQRKVYPSYVLVKMIITDESWYVVRNTKGVTGFVGPGSKPVP
                     LSEDEVKAMGIDTTDPKVVNSDIDFEIGDTVKVSQGPFSGQIGNIEEIDLENREVKVC
                     INAFGKRTLFVIELEGIEKI"
     gene            1245..1670
                     /gene="rplK"
                     /locus_tag="PC414_002963"
     CDS             1245..1670
                     /gene="rplK"
                     /locus_tag="PC414_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDF2465344.1"
                     /translation="MAKKVIGQIKLQIPAGKATPAPPVGPALGQHGVNIMGFTKEFNA
                     KTADQAGMIIPVVITVYQDRSFSFITKTPPAAVLIKKALNLKSGSGEPNKKKVAKMTS
                     AQVREIAELKMPDLNAASVEAAMSMIAGTARSMGVVIED"
     gene            1741..2439
                     /gene="rplA"
                     /locus_tag="PC414_002964"
     CDS             1741..2439
                     /gene="rplA"
                     /locus_tag="PC414_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDF2465345.1"
                     /translation="MAKKGKRYAGALQKVDRTKFYDASEALTLVSDIAGAKFDETVEA
                     HIKLGVDSRHADQQVRGAVVLPHGTGKTKRVLVFAKGEKAKEAEQAGADFVGAEELVQ
                     KIQGENWFDFDIVVATPDMMGVVGRLGRVLGPKGLMPNPKSGTVTFDVAKAIDEIKAG
                     KVEYRLDKTNIIHVPVGKVSFGGEKLTENFTALMDAIIKAKPAAAKGQYLRSITVASS
                     MGPGVKINPARTAE"
     misc_feature    2491..2619
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            2662..3168
                     /locus_tag="PC414_002965"
     CDS             2662..3168
                     /locus_tag="PC414_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDF2465346.1"
                     /translation="MRKAIEIKSEVVSEIVEKLQKSSAAVVVDYKGLTVEEVTELRKQ
                     MREAGVDYKVYKNTLVRRAAKEVGIEQFNDELLVGTNAIAFGYDDPVAPARILKGFMD
                     SHPKMKLKMGIVEGAFYDESKIVEMANIPSREVLIAKLLGSLKAPVSNFAYLIDAIAK
                     KAEGQEEA"
     gene            3226..3591
                     /gene="rplL"
                     /locus_tag="PC414_002966"
     CDS             3226..3591
                     /gene="rplL"
                     /locus_tag="PC414_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDF2465347.1"
                     /translation="MTIEQILEAIENMKVLELNELVKAAEEKFGVSASAPVMVAGAAA
                     GGPAAEEKTEFDVVLTDVGSSKVGVIKAVREITGLGLKEAKEVVDNAPKTVKEGASKE
                     EADQIKEKLEAAGAKVEVK"
     gene            3982..4770
                     /locus_tag="PC414_002967"
     CDS             3982..4770
                     /locus_tag="PC414_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDF2465348.1"
                     /translation="MEKLQGKIAVVTAATKGIGLASAEILAKNGATVYLAARSEELAN
                     EVINKISAEGGCAKFVYFNAREEETFTSMIEEVVKKEGKIDILVNNFGSTNPSLDKDL
                     VTGDTDNFFDTVNTNLKSVYLPCKAAIPHMIKNGKGSIVNISSIGSVLPDLSRIAYCV
                     SKAAINSLTQNIATQYAKDNVRCNAVLPGLIATKAALDNMSPEFIKEFLKHVPLNRIG
                     EPDDIAKAVLFYASDDSSFITGDLLEVAGGFGLPTPQFADNILG"
     gene            5241..8957
                     /gene="rpoB"
                     /locus_tag="PC414_002968"
     CDS             5241..8957
                     /gene="rpoB"
                     /locus_tag="PC414_002968"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDF2465349.1"
                     /translation="MPHPVTIGKRTRMSFSKIKEIADVPNLIEIQVDSYEWFLKEGLK
                     EVFDDISPIEDYTGNLILEFVDYSLDDKPKYDIEECKERDATYCAPLKVKVRLINKET
                     GEIKEQEVFMGDFPLMTERGTFVINGAERVIVSQLVRSPGVYYAEERDKTGKRLISST
                     VIPNRGAWLEYETDSNDVISVRVDRTRKQPVTVLLRALGIGTDAEIIDLLGEDERLSA
                     TLEKDNTKTVEEGLVEIYKKLRPGEPPTVESASSLLNALFFDPKRYDLAKVGRYKFNK
                     KLALCYRIMNKISAEDIINPETGEVFVKAGEKISYDLAKAIQNAGINVVNLLMDDDKK
                     VRVIGNNFVDIKSHIDFDIDDLNIKEKVHYPTLKEILDGYSDEEEIKEAIKSRIKELI
                     PKHILLDDIIASISYEFNIFYNIGNIDDIDHLGNRRIRSVGELLQNQVRIGLSRMERV
                     IKERMTVQDMEAITPQALVNIRPVSAAIKEFFGSSQLSQFMDQTNPLSELTHKRRLSA
                     LGPGGLSRERAGFEVRDVHHSHYGRMCPIETPEGPNIGLINSLGTYAKINEFGFIESP
                     YRKFDKETSTVTDEIHYLTADEEDLFVRAQANEPLTEDGKFVNHRVVCRTVNGAVEMV
                     PESRVDYMDISPKQVVSVATAMIPFLENDDANRALMGANMQRQAVPLVRREAPIIGTG
                     IEYRAAKDSGAVVVARNSGIAERVTADEIIIKREDGNRDRYNLLKFKRSNSGTCINQT
                     PIINKGDQIIKGDVIADGPATDLGEVALGRNCLIAFMTWEGYNYEDAILINERLVKED
                     RLSTIHIEEYECEARDTKLGPEEITRDIPNVGDSAIKNLDDRGIIRIGAEVDSGDILV
                     GKVTPKGETELTAEERLLRAIFGEKAREVRDTSLKVPHGESGIIVDVKVFTRENGDDL
                     SPGVNELVRCYIAKKRKIKVGDKMAGRHGNKGVISRVLPEEDMPFMENGTPLDIILNP
                     QGIPSRMNIGQVLEVHLGLAAKTLGWYVATSVFDGANEYDIMDALEEAGYPRDGKLTL
                     YDGRTGESFDNRITVGYMYYLKLHHLVDEKLHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAHILQEILTVKSDDVVGRVRTYEAIVKGENIPEPGIPESFKVLIK
                     ELQSLCLDVKVLTDEDQEIEVRESVDEDDTIGEFELDVVNHMGEVEESNIIEEIEDDF
                     AENAEDEDIENLEEFTEDDLFEEEIDFDSDDFDM"
     gene            8999..12484
                     /gene="rpoC"
                     /locus_tag="PC414_002969"
     CDS             8999..12484
                     /gene="rpoC"
                     /locus_tag="PC414_002969"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HDF2465350.1"
                     /translation="MFELNNFESIKIALASPEKIRQWSRGEVKKPETINYRTLKPEKD
                     GLFCERIFGPQKDWECHCGKYRRVRYKGVVCDRCGVEVTKSKVRRERMGHIELAAPMS
                     HIWYFKGIPSRMGLLLDMSPRSLEKILYFASYVVVDPGETGLNEKQLLTEKEYRTALE
                     KYGYTFTVGMGAEAVKTLLQNIDLEQQSKDLRAELKDSTGQKKVRTIRRLEVVEAFKK
                     SGNKPEWMILDAIPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLELGA
                     PDIIVRNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPELKFYQCGLPKKMALELFKPFVMDKLVKEGYAHNIKSAKSIVEK
                     VKPEVWDVLEDVIKSHPVLLNRAPTLHRLGIQAFEPILVEGKAIKLHPLVCTAYNADF
                     DGDQMAVHVPLSVEAQAEARFLMLSVNNILAPKDGSPITTPSQDMVLGCYYLTIEAQD
                     GAKGTGMVFKDFNELLLAYYNKSVHLHALVKLKVTLEDGRSSLVESTVGRFIFNENIP
                     QDLGFVDRKENPFALEVDFLADKKSLGKIIDKCFRKHGNTETAELLDYIKALGFKYST
                     LGGITVAVDDMSVPEEKKVFIAEAEAKVDKYEKAYRRGLISDEERYEKVIETWTETTD
                     KVTDALMGGLDRLNNIYIMAHSGARGSKNQIRQLAGMRGLMANASGKTVEIPVKSNFR
                     EGLSVLEYFTSSHGARKGLADTAIRTAESGYLTRRLVDVSQDVIVREIDCGTEDTTEI
                     YAIKEGNEVIEEIYDRIVGRYTIDPILNPETGEVIVEADSMIQEDEAETIVALGIEKI
                     RIRTVLNCKTNHGVCSKCYGRNLATGKEVNIGEAVGIIAAQSIGEPGTQLTMRTFHTG
                     GVAGADITQGLPRVEELFEARKPKGLAVITEVSGRVEIDETGKRKEVNVIPEEGETQT
                     YVIPYGSRLKVKQGQMLEAGDPLTQGFINPHDIVRVNGVKGVQEYIVKEVQRVYRLQG
                     VDVNDKHIEVIVRQMLSKVKVEDPGDTDLLPGGYEDVLTFNECNKDAIDKGLRPAVAK
                     RVLLGITKASLATDSFLSAASFQETTRVLTEAAIKGKEDHLIGLKENVILGKLIPAGT
                     GMKKYRNIAVEKIED"
     gene            12773..13195
                     /gene="rpsL"
                     /locus_tag="PC414_002970"
     CDS             12773..13195
                     /gene="rpsL"
                     /locus_tag="PC414_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HDF2465351.1"
                     /translation="MPTINQLVRKSRKALEKKSTAPALQKGYNSLNKKVTDASAPQKR
                     GVCTSVKTVTPKKPNSALRKVARVRLTNGIEVSAYIPGEGHNLQEHSVVLIRGGRVKD
                     LPGVRYHILRGTLDTAGVDKRRQSRSKYGAKRPKEAKK"
     gene            13319..13789
                     /gene="rpsG"
                     /locus_tag="PC414_002971"
     CDS             13319..13789
                     /gene="rpsG"
                     /locus_tag="PC414_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HDF2465352.1"
                     /translation="MPRKGNIPKREVLPDPMYGSKVVTKLINNLMVDGKKGKSQRIVY
                     DAFAIVAEKTGEEALEVFNKAMDNIMPVLEVKARRVGGANYQVPIEVRPERRQTLGLR
                     WLVKYTRARGEKGMVEKLAKEIMDAANNTGASVKKKEDTHKMAEANKAFAHYRW"
     gene            13838..15904
                     /gene="fusA"
                     /locus_tag="PC414_002972"
     CDS             13838..15904
                     /gene="fusA"
                     /locus_tag="PC414_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDF2465353.1"
                     /translation="MARKFPLERTRNIGIMAHIDAGKTTTTERILFYTGQTHKIGETH
                     EGASQMDWMEQEKERGITITSAATTASWKDHRINIIDTPGHVDFTVEVERSLRVLDGS
                     VAVFCAKGGVEPQSENVWRQAETYGVPRIAFVNKMDILGADFYNVVSMMKSRLNSNAV
                     PMQLPIGKEDSFIGIIDLLKMDAVIYKDDLGVEMEETDIPEDMKELAAEWREKLVESV
                     AETDEELMMKYLEGEELTIDELKVAIRKATIACEMNPVFCGTAYRNKGVQLVIDAVLD
                     YLPAPTDIPAIKGILADGEEAERHSSDEEPFSALAFKIMTDPFVGKLAFFRVYSGTLE
                     SGSYVLNATKNKRERIGRILQMHANTREEITKVYAGDIAAAVGLKDTTTGDTLCDPAN
                     PIILESMEFPEPVISVAIEPSSKAAQEKMGIALQKLAEEDPTFTVKTDQETGQTIISG
                     MGELHLEIIVDRLLREFKVEAKVGAPQVAYRETITQPVDVEYKYSKQSGGRGQYGHVK
                     IRVAPQEPGEGYKFTNKTVGGSVPKEYVGPVDMGIQGAMQSGIVAGYPVVDVAVELYD
                     GSYHEVDSSEMAFKMAGSMAFKDAMKKGNAVLLEPYFKVEVVTPEDYMGDVMGDLNSR
                     RGLIQGMEARSGAQVINAFVPLSEMFGYSTDLRSSTQGRATYTMIFDHYEQVPASVAK
                     KIAEGK"
BASE COUNT         6257 a         1989 c         3249 g         4483 t
ORIGIN      
        1 ctttactaaa ctgatggatt aaaaggaata acaaaaagag gggttatgct cctctttttt
       61 taacatagaa atacaaaaac attgacacag cataggattt tgtgataact tatacaaggt
      121 aatgattata ggaagttaat aatatcttga atactgtaga tttttaataa atacatattt
      181 taaagatatt atattcacat atattaacaa tatatattat tgtattacaa agttggaggt
      241 gtatcagatg agagttaaag taactttagc atgtacagag tgtaagcaaa gaaactacaa
      301 cactactaag aataagaaaa ataatccaga cagaatagaa ttacaaaaat attgtagatt
      361 ctgtaaaaag catacaactc ataaagaaac aaaatagttt tttataagga tgtgagtatg
      421 gaatggctgc ccaaaaaaat gacggtgcaa aaactaaaaa aaggtttagt ttgtttggat
      481 acttaaaaga aactaaacaa gaattaaaaa gagttacatg gccgacaaaa aaggaactgt
      541 ttaaaaacac aagtatagtt ttaactgtag ttatttcatg cactatatta gtatggggta
      601 tagacactat attatctggt gcactagcgt tattactaaa atagacgaaa agaggtaagt
      661 taaatgtcag aattacaaga agcaagttgg tatgtagttc atacctattc gggacatgaa
      721 aataaagtta aagctacaat tgagaaagca gttaaaacaa gaggtatgga agactgcata
      781 agacaagtag ttgttccaac agaagaagta gtggaaacta ctaaaactgg aaaagaaaaa
      841 actagacaac gtaaagttta tccaagttat gttttggtta aaatgataat aactgatgaa
      901 tcttggtatg tagtaagaaa tactaaggga gttacaggtt tcgttggacc tggttcaaaa
      961 ccagtgccat taagtgaaga cgaagtcaaa gctatgggga tagacacaac agaccctaaa
     1021 gtagtaaata gcgatattga ttttgaaata ggagatacag taaaagtatc tcaaggacca
     1081 tttagtggac aaataggtaa catagaagaa attgacttag aaaatagaga agtcaaagta
     1141 tgcataaatg catttggtaa aaggactcta tttgtaatag agcttgaagg tatagaaaaa
     1201 atctaatact atattataga aaaatctgag gaggtgcgct caaaatggct aaaaaagtta
     1261 taggtcaaat aaaattacaa atacctgcag gaaaggctac tccagcgcca ccagttggac
     1321 cagcattagg acaacatggt gttaatataa tgggattcac aaaagaattt aatgctaaaa
     1381 ctgcagatca agctggaatg ataataccag ttgttataac tgtatatcaa gatagatcat
     1441 tcagttttat aacaaaaact ccaccagctg cagttttaat taaaaaagca ttaaacttaa
     1501 aatcaggttc tggagaacca aacaagaaaa aagttgctaa aatgacttca gctcaagtaa
     1561 gagaaatagc tgaattaaag atgcctgact taaatgctgc atcagtagaa gctgctatga
     1621 gtatgatagc tggtactgct agaagcatgg gtgttgtaat cgaagactaa ttaaaagata
     1681 cacttttttt tataaagtgg gaggctagaa gccgaaatta accacgagga ggaaaataaa
     1741 atggctaaaa aaggtaaaag atatgcaggt gcattacaaa aagtagatag aactaaattt
     1801 tatgatgcat cagaagcatt aacattagtt tcagatatag ctggagctaa atttgatgag
     1861 acagtagaag cacacattaa attaggtgtt gactcaagac atgctgacca acaagtaaga
     1921 ggtgcagttg tattacctca tggaactggt aaaacaaaaa gagttttagt ttttgctaaa
     1981 ggtgaaaaag ctaaagaagc tgaacaagct ggtgctgact ttgtaggagc tgaagaatta
     2041 gttcaaaaaa tacaaggtga aaactggttt gactttgata tagtagttgc tactccagat
     2101 atgatgggtg tagtaggtag attaggtaga gtattaggac ctaaaggttt aatgccaaac
     2161 cctaaatcag gaacagttac atttgatgta gctaaagcta tagatgaaat aaaagctggt
     2221 aaagttgaat acagattaga caaaactaac ataatacacg ttccagtagg aaaagtatca
     2281 tttggtggag aaaaattaac tgaaaacttt actgcattaa tggatgctat aataaaagct
     2341 aaaccagctg ctgctaaagg acaatattta agaagtataa cagtagcttc ttcaatggga
     2401 cctggagtta aaataaaccc agcaagaaca gctgaataat aaaactgttg acacaggttg
     2461 acaaaaatgt tattatatca cttgtcaatg taaaaaagaa tagatttgcc gtagatagtg
     2521 ggtgcgaaag cttaatatcc cgccgaggtt ttagataaat tgagattact aaagcttttg
     2581 atgtctactt ttggcaacaa aagcttttta taatgttgcg gtaaatacta agcctatttg
     2641 gtgaaggagg tgcacatcta tatgagaaaa gctatagaaa ttaaatcaga agttgtttct
     2701 gagatagttg aaaagttaca aaaatcttct gctgctgttg ttgtagatta caaaggatta
     2761 acagttgaag aagtaactga gttaagaaaa caaatgagag aagctggtgt agattacaaa
     2821 gtatacaaaa atactttagt tagaagagca gctaaagaag ttggtataga gcaatttaat
     2881 gatgaattat tagttggaac taatgccata gcatttggat atgatgatcc agttgctcca
     2941 gctagaatat taaaaggatt catggattct catccaaaaa tgaaattaaa gatgggtatt
     3001 gtggaaggtg cattctatga tgaatctaag attgttgaaa tggctaacat accatcaaga
     3061 gaagttctta tcgctaaatt acttggtagc ttaaaagctc cagtatcaaa cttcgcatac
     3121 ttaatagatg caatagcgaa aaaggcagaa ggtcaagaag aagcttaatt gcttaactaa
     3181 aaaattataa atattaattt gaaataaaat tcggaggtgc taaaaatgac aatagaacaa
     3241 atattagaag ctatagaaaa tatgaaagtt ttagaattaa atgaattagt taaagctgct
     3301 gaagaaaaat tcggtgtatc agcttcagct ccagtaatgg tagctggtgc agctgctggt
     3361 ggaccagctg ctgaagaaaa aactgagttt gatgtagtat taactgacgt tggttcttca
     3421 aaagttggag ttataaaagc agttagagaa ataacaggat taggattaaa agaagctaaa
     3481 gaagtagttg acaatgctcc taagacagtt aaagaaggag cttctaaaga agaagctgat
     3541 caaataaaag agaaattaga agctgctgga gctaaagtag aagttaagta gtttctttaa
     3601 aaagataccc attgggtatc tttttttatt tataaaaaac tataaagaaa gtatttggaa
     3661 ttataaagaa agtattttta taaaataaaa aaaatttata taatagtact aaattaaggg
     3721 tatcatatat ggtgtaatgg tattttaatt taacattatt atcaaattaa aatataaaca
     3781 attgttgtaa tgtctgtata taaagtatat aattaaacta aaatgtaaaa ttttacattt
     3841 tagtttaaaa aaatacacga gtgtttataa ataaaatgta ataaataatt aattaacaaa
     3901 tataaatttt aaaataagtt agatatttag aaaaatcgaa atttgtagga tttaaaggac
     3961 atattcaagg aggagttaat aatggaaaaa ttacaaggaa aaattgcagt agttactgca
     4021 gcaacaaaag gtattggatt agcatcagca gagatattag caaaaaatgg agcaactgtg
     4081 tacttagcag ctcgttcaga agaattagct aatgaagtta taaataaaat aagtgcagaa
     4141 ggtggttgtg ctaagtttgt ttactttaat gctcgtgaag aagagacctt tacctcaatg
     4201 atagaagagg tagttaaaaa agaaggtaag atagatatac ttgtaaacaa ttttggttca
     4261 acaaaccctt ctcttgacaa agaccttgta actggggata cagataattt ctttgacaca
     4321 gtaaatacta atttaaaaag tgtgtattta ccatgtaaag cagcaattcc tcatatgata
     4381 aagaatggaa aaggaagtat agtaaatata tcaagtatag gttcagtatt acctgattta
     4441 tctagaatag cttactgtgt atcaaaagca gcaatcaact cattaactca aaacatagct
     4501 acacaatatg ctaaagacaa tgttagatgt aatgctgtac ttccagggct tattgcaact
     4561 aaagcagcat tagacaatat gtctccagag ttcataaaag aatttttaaa gcatgttcct
     4621 ttaaatcgta taggggaacc agatgatata gcaaaagctg ttctattcta tgctagtgat
     4681 gactcatcat ttataacagg ggatttactt gaagttgcag gaggatttgg tttacctact
     4741 ccacaatttg cagataatat attaggataa ttaaattact aatatgcaat gaaagcttat
     4801 gcattagtta tagtagaaat gatattttta taaaggccat agatataatt tatattggaa
     4861 aggtttgagg ttcaaaattc caagttaaat ttatttatgg tctttatttt gtatatttta
     4921 ataaattgta tatatttgta taaatgtaat tatttacaat cattttaata tggaattata
     4981 attaaatagg ggggatggta cttgtataaa attttttata gaaaattaga cctataaatg
     5041 aaaaatatgg attttatatt ggaaaattgt acataataca taatatatgc ttaaaataat
     5101 ttacaaaaaa tcaaaaataa tgcatagttt acatataaga taaaaaatat attgaaaaat
     5161 acttgacatt atgctaaaat tattagatgc gttagaatga actattgttt tatttttaat
     5221 tattgagagg tgaaaagtga atgccacatc ctgtcacgat aggtaaaaga actagaatga
     5281 gcttttctaa aattaaggaa atagctgatg tgccaaatct tatagaaatt caagtagatt
     5341 cctatgagtg gtttttaaaa gaaggtttaa aagaagtgtt tgatgatatt tcaccaatag
     5401 aagattatac tggtaatctt atattggaat ttgtcgacta ttctctagat gataaaccaa
     5461 agtacgatat agaagagtgt aaagagagag atgctacata ttgtgcacct ctaaaggtta
     5521 aggtaagact tataaataaa gaaacaggtg agataaaaga acaagaagtg tttatgggtg
     5581 acttcccttt aatgacagaa agaggaactt tcgttataaa tggagcagaa agagttatag
     5641 taagtcaatt agttagatct cctggtgttt actatgcgga agaaagagat aaaacaggta
     5701 agagattgat ttcatctact gtaattccta atagaggagc atggttagaa tatgaaacag
     5761 attctaatga tgtaatatct gtaagagttg atagaacaag aaaacaacca gttacagttt
     5821 tacttagagc tttaggaata ggaacagatg cagagataat tgacctttta ggagaagatg
     5881 agagattatc tgctacatta gaaaaagata atactaagac agtagaagaa ggtcttgtag
     5941 agatatacaa aaaattaaga ccaggtgaac ctcctactgt agaaagtgca tcatctttat
     6001 taaatgcttt attctttgac ccaaaaagat atgacctagc aaaagttggt agatataaat
     6061 tcaataagaa acttgcttta tgttatagaa taatgaataa aatttcagca gaagacataa
     6121 taaatccaga aactggagaa gtatttgtaa aagctggaga aaaaataagc tatgatttgg
     6181 ctaaagccat acaaaatgca ggtataaatg tagtaaatct actaatggat gatgataaaa
     6241 aagttagagt tataggaaat aattttgtgg atattaagtc tcacattgat tttgacatag
     6301 atgatttaaa cataaaagaa aaagtacatt atccaacttt aaaagaaatt ttagatggat
     6361 atagtgatga agaagaaata aaagaggcta taaaatcaag aattaaagaa cttataccaa
     6421 aacatatatt attagatgat ataatagctt caataagtta tgagttcaat atattctata
     6481 atataggaaa tattgatgat atagatcacc taggaaacag aagaataaga tctgtaggtg
     6541 aattgttaca aaatcaagtt agaattggtc tttcaagaat ggaaagagtt ataaaagaaa
     6601 gaatgacggt tcaagatatg gaagctataa cgcctcaagc gttagttaac ataagaccag
     6661 tttcagctgc aataaaagaa ttttttggta gttctcagtt atctcagttc atggatcaaa
     6721 caaatcctct atctgagtta acgcataaga gaagattatc agcccttggg cctggaggtc
     6781 tttcaagaga aagagctgga ttcgaagtgc gtgacgttca ccattcacac tatggtagaa
     6841 tgtgtccaat agagactcca gagggaccaa atatcggtct tataaactct ctaggaactt
     6901 atgcaaaaat aaatgaattt ggatttatag aatcaccata tagaaaattt gataaagaaa
     6961 catcaacagt tactgatgaa atacattatt taactgctga cgaagaagat ttattcgtaa
     7021 gagctcaggc aaatgagcca ttaacagaag atggtaaatt cgtaaaccat agagttgttt
     7081 gtagaactgt aaatggtgct gttgaaatgg ttcctgaaag tagagtagat tacatggata
     7141 tatctcctaa gcaggttgta tctgttgcca ctgctatgat acctttctta gaaaatgatg
     7201 atgccaaccg tgcccttatg ggagcgaaca tgcagcgtca ggcagtgcct ctagttagaa
     7261 gagaagcacc aattatagga actggtatag aatacagagc agcaaaagac tctggagcag
     7321 ttgttgttgc tagaaattct ggtatagcag aaagagtaac agcagatgaa ataattataa
     7381 aaagagaaga tggaaacaga gatagatata atctacttaa gtttaaacgt tcaaactcag
     7441 gtacttgtat aaatcaaaca cctataataa ataagggtga ccagataata aaaggtgatg
     7501 ttatagcaga tggtccagca actgatttag gagaagttgc acttggaaga aactgtctta
     7561 tagcattcat gacttgggaa ggttataact acgaggatgc tatattaata aatgaaagat
     7621 tagttaaaga agatagatta tcaacaattc atatagaaga atatgaatgt gaagctagag
     7681 atacaaaact agggccagag gaaataacta gagatatacc taatgttgga gatagtgcaa
     7741 ttaagaactt agatgacaga ggtataataa gaataggtgc agaagtagac tcaggtgata
     7801 tattagttgg taaagtaact ccaaaaggag aaactgaact tactgctgag gaaagattac
     7861 ttcgtgcaat atttggagaa aaggctagag aagttagaga tacttcactt aaagtgcctc
     7921 atggtgaatc tggtataata gttgatgtaa aagtatttac tagagaaaat ggagatgatt
     7981 tatctccagg agtaaatgaa ttagtaagat gctatatagc taaaaagaga aaaataaaag
     8041 ttggagataa aatggctggt cgtcatggta ataaaggggt tatctcaaga gtattacctg
     8101 aagaagatat gccattcatg gaaaatggaa caccactaga cataatactt aacccacaag
     8161 gtataccatc acgtatgaac attggtcagg ttttagaagt tcatttagga cttgctgcta
     8221 agacattagg atggtatgta gcaacatcag tattcgatgg tgctaatgag tatgacataa
     8281 tggatgctct tgaagaagct gggtatccta gagatggtaa attaacttta tatgatggta
     8341 gaacaggtga gtcttttgac aacagaataa ctgttggata tatgtactac ttgaaactac
     8401 atcacttagt agatgaaaaa ctacatgcaa gaagtactgg accatactca ttagttactc
     8461 aacagccact aggtggtaaa gcacaatttg gtggacaaag atttggagag atggaggttt
     8521 gggctctgga agcatatgga gctgctcaca tacttcaaga aatacttact gttaagtctg
     8581 atgacgttgt aggtcgtgtt agaacatatg aagctatagt taaaggtgaa aatatacctg
     8641 aaccaggaat cccagaatca tttaaggttc ttataaaaga acttcaaagt ttatgcttag
     8701 atgtaaaagt attgacagat gaagaccaag aaatagaagt aagagaatct gtggatgaag
     8761 atgatacaat aggtgaattt gagctagatg ttgtaaatca tatgggagaa gttgaagaaa
     8821 gtaatataat agaagaaatt gaagatgatt ttgcagaaaa tgcagaagat gaggatatag
     8881 agaatttaga agaattcact gaagatgatt tatttgaaga agaaatagat tttgatagtg
     8941 atgactttga tatgtaaatt taattaatgt aaatctcaaa tagaagggag agaactcctt
     9001 gtttgaatta aacaatttcg agtcgataaa aatagcattg gcttctccag aaaaaataag
     9061 acaatggtct aggggagaag ttaaaaagcc agaaactata aattaccgta ctttaaaacc
     9121 agaaaaagat ggtcttttct gtgaaagaat atttggacca caaaaagact gggagtgcca
     9181 ctgtggtaaa tatagaagag ttagatataa aggtgtagtt tgtgatagat gtggagtaga
     9241 agtaactaaa tcaaaagtaa gaagagagag aatgggacat atagagctag ctgctcctat
     9301 gtctcacatc tggtacttca aaggtatacc aagtagaatg ggacttttac ttgatatgtc
     9361 accaagatcg ttagaaaaaa tattatactt tgcctcatat gtggtagttg atccaggaga
     9421 gactggatta aacgaaaaac aattgcttac agaaaaagaa tacagaactg ctcttgaaaa
     9481 gtatggatat acttttactg taggaatggg tgctgaagct gtaaaaacat tactacaaaa
     9541 tatagattta gagcaacaaa gtaaagacct aagagcggag ttaaaagata gtacagggca
     9601 aaagaaagtt agaacaataa gaagattaga agttgtagag gcatttaaaa agtctggaaa
     9661 taaaccagaa tggatgattt tagatgcaat accagtaata ccaccagatt taagaccaat
     9721 ggtacaactt gatggtggaa gatttgcgac ttcagaccta aatgatttat atagaagagt
     9781 tataaataga aacaacagac ttaaaagatt attagagctt ggagctccag atataattgt
     9841 aagaaatgaa aaaagaatgc ttcaagaagc tgttgatgca ttgatagata atggtagaag
     9901 aggtagacct gtaacaggac ctggaaatag accacttaaa tctttatcag atatgttaaa
     9961 aggtaagcaa ggtcgtttcc gtcaaaactt acttggtaag cgtgttgact actcaggacg
    10021 ttctgttata gttgttggac cagaacttaa attttatcaa tgtggacttc caaagaagat
    10081 ggcattagaa ttattcaagc catttgttat ggataaatta gttaaagaag gttatgcaca
    10141 taatataaaa agtgcgaaat ctatagtaga aaaagttaag ccagaagttt gggatgtttt
    10201 agaagatgtt ataaaaagtc atccagttct tcttaaccgt gcaccgactc tacatagatt
    10261 aggtatacaa gcatttgaac caatcttagt tgaaggtaag gctataaaac tacatcctct
    10321 tgtatgtaca gcttataatg cagactttga tggtgaccaa atggcagttc atgtaccttt
    10381 atcagtagaa gcacaagcag aagcaagatt cttaatgctt tctgtaaata atatacttgc
    10441 tcctaaagat ggttcaccta taactactcc atctcaggat atggttttag gttgctatta
    10501 tctaacaata gaagcccaag atggagctaa aggaacaggt atggtattta aagactttaa
    10561 tgaactatta cttgcttatt acaataaatc agttcatcta catgcattag tgaaacttaa
    10621 ggtaacactt gaagatggaa gaagttcatt ggttgaaagt actgttggta gatttatatt
    10681 taatgaaaat atacctcaag acttaggttt tgtagataga aaagaaaatc catttgcact
    10741 tgaagttgat ttcttagcag ataaaaaatc tcttggtaaa ataatagata aatgctttag
    10801 aaaacacgga aatacagaaa cagctgaatt actagattat ataaaagctc taggatttaa
    10861 atattctaca ttaggtggca taacagttgc tgttgatgat atgagtgtcc cagaagaaaa
    10921 gaaagtattt atagctgagg cagaagctaa agttgataag tatgaaaaag catacagaag
    10981 aggtctaatc tctgatgaag aaagatatga aaaagttata gagacatgga cagaaacaac
    11041 tgataaagtt actgatgctc ttatgggtgg actagataga ttaaataata tatatataat
    11101 ggcacattca ggagccagag gttctaaaaa ccaaattaga cagctggcag gtatgcgtgg
    11161 tcttatggca aatgcatctg gtaaaacagt tgagatacca gttaaatcta atttccgtga
    11221 aggtttatca gtactagaat actttacatc ttcacatggt gccagaaaag gtcttgccga
    11281 tacagctata cgtacagctg aatctggata tttaacaaga agacttgttg atgtaagtca
    11341 agatgttatt gtaagggaaa tagattgtgg aacagaagat actacagaga tttatgctat
    11401 aaaagaagga aatgaagtta tagaagagat atatgataga attgtaggaa gatatactat
    11461 agaccctata ttaaatcctg aaactggaga agttatagtt gaggctgatt caatgataca
    11521 agaagatgaa gcagaaacta tagtagcttt aggaattgaa aaaattagga taagaacagt
    11581 tcttaactgt aaaactaatc atggagtttg ttctaagtgt tatggtagaa acttggcaac
    11641 aggaaaagaa gttaatatag gtgaagcagt tggtataata gcagctcaat ctatcggtga
    11701 gccgggtact cagcttacaa tgcgtacatt ccatactgga ggagttgcag gagctgatat
    11761 aactcaaggt ctaccaaggg ttgaagaatt atttgaagct agaaaaccaa aaggattagc
    11821 tgtaataact gaagtatctg gtagagttga aattgatgaa acaggaaaga gaaaagaagt
    11881 aaatgtaata ccagaagaag gcgagactca aacatatgta attccatatg gttctagatt
    11941 aaaagttaag caaggtcaaa tgctagaggc tggagaccct ctaacacaag gatttataaa
    12001 tcctcatgat atagtaagag taaatggtgt taagggagtt caagaatata tagttaaaga
    12061 agttcaaaga gtgtatagac ttcaaggggt tgatgttaat gataagcata tagaagttat
    12121 agtaagacaa atgctatcta aagtaaaagt tgaagaccca ggagatacag atttattgcc
    12181 aggtggatat gaagatgtat taacattcaa tgaatgtaat aaagatgcta tagataaagg
    12241 tctaagacca gcagttgcta aaagagtttt acttggtata actaaagcat ctcttgcaac
    12301 tgattcattc ttatcagcag cttctttcca agaaacaaca agagtattaa cagaggcagc
    12361 aataaaaggt aaagaagacc acttaatagg acttaaagaa aatgttatat taggtaagtt
    12421 aataccagca ggaacaggaa tgaagaaata tagaaatata gctgttgaaa aaattgaaga
    12481 ttaatagcat atagagtaaa gaaatcttga caaccattat ttgtggtgtt aaaatagtaa
    12541 agtgtgttgt ttaaaagtac actaagggct tgaacaagtt cttagtgact tttagatggc
    12601 aataaatgag aactaaaaag catcaagcta gaaaagtgct aatatcacat atttatgtgt
    12661 tatagacatt aaattgtaag taacgaatgt tacaatcagt cagtatgaat attcatattg
    12721 atgtagttgg cggtcactga taagatcgaa aatttggaag gaggtgcaga tgatgccaac
    12781 aattaaccaa ttagttcgta aaagtagaaa agcgctagag aagaaatcta ctgctccagc
    12841 attacaaaaa ggttacaact ctttaaataa gaaagtaact gacgctagtg ccccacaaaa
    12901 aagaggggtt tgtacttcag taaaaacagt tactcctaag aagcctaact cagctttaag
    12961 aaaagttgcc agagttagat taactaatgg tatagaagtt tctgcttata taccaggaga
    13021 aggacacaac ttacaagagc atagtgttgt tcttataaga ggaggaagag taaaagacct
    13081 tccaggggtt agataccata tactaagagg tactctagat acagcaggtg ttgataagag
    13141 aagacaatca agatctaaat acggtgcgaa gagacctaaa gaagctaaaa agtaaaaaca
    13201 attaatgatt gattatagtt cggtgcttta aatatatata tatttacggc atcacggcat
    13261 taaatgattt atgtcgagta cctatgattt aagttaaata attaaggagg gaagcgaaat
    13321 gccaagaaaa ggtaatattc caaaaagaga agttttacca gatcctatgt atgggagtaa
    13381 ggtagttact aaattaataa ataatctaat ggtagacgga aaaaaaggaa agtctcaaag
    13441 aatagtttat gatgctttcg caattgttgc tgaaaagaca ggagaagaag ctttagaagt
    13501 atttaataaa gcaatggata atataatgcc tgttttagaa gtaaaagcaa gaagagttgg
    13561 tggggcaaac taccaagtgc caatcgaagt tagacctgaa agaagacaaa cattaggttt
    13621 aagatggcta gtaaaatata ctagagctcg tggagaaaaa ggaatggttg aaaagctagc
    13681 taaagagata atggacgcag ctaacaacac tggagcttca gttaagaaaa aagaagatac
    13741 tcataaaatg gcagaagcta ataaagcatt tgctcattac agatggtagg atatatagat
    13801 atcttataaa attgaggata tcataaggag gataaccatg gctagaaagt ttcctttgga
    13861 aagaactagg aatataggaa tcatggctca tatagatgca ggaaaaacta ctactacaga
    13921 aagaatcctg ttttatactg ggcaaactca taaaatagga gaaactcatg aaggagcttc
    13981 tcaaatggac tggatggagc aagaaaagga aagaggtata acaataactt ctgccgctac
    14041 tactgcttca tggaaagacc atagaataaa cataatagat actcctggac acgtggactt
    14101 cacagttgag gttgaaagat ctctaagagt tcttgacggt tcagttgctg tattctgtgc
    14161 aaaaggtggg gttgagcctc aatcagaaaa tgtatggaga caagctgaaa cttatggagt
    14221 acctagaata gcttttgtaa ataaaatgga tatattaggt gcagatttct acaatgttgt
    14281 aagcatgatg aaatctagat taaattctaa tgcagttcct atgcaattac caataggtaa
    14341 agaagattca tttataggta taatagactt attaaaaatg gatgctgtta tatacaaaga
    14401 cgacctaggg gttgaaatgg aagaaacaga tataccagaa gatatgaaag aattagctgc
    14461 tgagtggaga gaaaaattag ttgaatcagt agctgaaact gatgaagaat taatgatgaa
    14521 ataccttgaa ggtgaagaac ttactataga tgaattaaaa gttgctataa gaaaagctac
    14581 tatagcttgt gaaatgaacc cagttttctg tggtacagct tatagaaaca aaggtgttca
    14641 gttagtaata gatgcagttt tagattactt accagcgcca actgatatac cagctataaa
    14701 aggtatatta gcagatggag aagaagctga aagacattct tctgatgaag agccattctc
    14761 tgcattagca ttcaaaataa tgactgaccc attcgttgga aaactagcgt tcttcagagt
    14821 ttattctgga acattagaat caggttctta tgttttaaat gcaactaaga ataagagaga
    14881 aagaattggt cgtatattac aaatgcatgc taatacaaga gaagaaataa ctaaagtgta
    14941 tgctggagat atagcagcag cagtaggatt aaaagatact actactggag acacattatg
    15001 tgaccctgct aatccaataa tacttgaatc tatggaattc cctgagccag ttatatctgt
    15061 tgctatagaa ccaagttcta aagcagctca agaaaaaatg ggtatagctc ttcaaaaatt
    15121 agctgaagaa gacccaactt tcacagttaa gactgaccaa gaaacaggac aaactataat
    15181 atctggtatg ggtgaattac acttagagat aatagtagat agattattaa gagaatttaa
    15241 agtagaagct aaagttggtg caccacaagt tgcatataga gagactataa ctcaaccagt
    15301 tgatgttgaa tacaagtact ctaagcaatc aggtggtaga ggacaatacg gtcacgttaa
    15361 gattagagtt gctcctcaag agccaggtga aggatacaaa ttcacaaaca aaactgttgg
    15421 tggatcagta ccaaaagaat atgttggtcc agttgacatg ggtatacaag gagctatgca
    15481 atctggtata gttgctggat atccagttgt agacgttgct gttgagttat atgatggttc
    15541 ttaccatgag gttgactctt ctgagatggc gtttaagatg gctggttcaa tggcattcaa
    15601 agatgctatg aagaaaggta atgcagttct tttagagcct tacttcaaag tagaagttgt
    15661 tactccagaa gattacatgg gagatgttat gggtgactta aactcaagaa gaggattaat
    15721 ccaaggtatg gaagctagaa gtggtgcaca agttataaat gcattcgttc cactttcaga
    15781 aatgtttgga tactcaacag acctaagatc ttcaactcaa ggtcgtgcaa cttacacaat
    15841 gatattcgat cactatgagc aagttccagc ttcagttgct aagaaaatag ctgaaggaaa
    15901 ataatttaaa aataattaat aatataaata taaaaaataa tcagctagat gagagatatc
    15961 tctcatctag cacaagat
//
LOCUS       DAMJVK010000093        15933 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.096, whole genome shotgun sequence.
ACCESSION   DAMJVK010000093 DAMJVK010000000
VERSION     DAMJVK010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15933
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.096"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            55..651
                     /locus_tag="PC414_002973"
     CDS             55..651
                     /locus_tag="PC414_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3793 family protein"
                     /protein_id="HDF2465354.1"
                     /translation="MSCCNSQFCCKNKIESSYIKWLLEVLGPVFLGSKPAEIINISFT
                     DVNREEKINDIYKYLYKCKKIDFIVIDKEKKGLKILFVNKKALSEKLKCKKTVNFLKF
                     LGYKQNTNVNAYLEHLVDKLKSDVFPDEIGIFLGYPLKDVIGFMGYSNYEVSMIKYWK
                     VYGDTKQSEDTYSKFLLHRKKMRKLLDYISVDKIVSCF"
     gene            complement(749..1708)
                     /locus_tag="PC414_002974"
     CDS             complement(749..1708)
                     /locus_tag="PC414_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2465355.1"
                     /translation="MTSLIMVLVGTVGCSTTNDKKDEPAKKEASLPKEINLTYVKSPL
                     NVPSILEKKDDLFGKEFKKDNIKVNFHELTTGPEQTQALAAGELDFLHALGGTSAIIA
                     ASNGVGLKITNIYSRSPKGYMLITNNDNIKSIKDLKGKKIVGPKGTILHQLLIAALAK
                     EGYTIDDVEYVNMDIPSAASALESKSADVAMLAGPVALKTINSGAKMIVNGEGLVSGI
                     IVTAVSDDFAEKYPELVKRFMKVHEETLKYMNENKDEVMDVVSKEVGLSLDETKEMYS
                     WYDFSSKITDKDIKELEDTQEFLMSNGMQQKKINIKDMLYNQN"
     gene            complement(1742..2473)
                     /locus_tag="PC414_002975"
     CDS             complement(1742..2473)
                     /locus_tag="PC414_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2465356.1"
                     /translation="MVRSGFLLENIYKKYLVDNKEHLVLDNISLNISSEEITVILGES
                     GCGKTTLLRILAGLENATSGNVYFFNNDKKCTPKVGMVFQESRLMPWLNVSENILLHT
                     EKDNRNKVDLDKYLKMMKLEKFKNSYPNELSGGMAHRVSIARALSFNPDILLMDEPFA
                     ALDYFTRRKMQKEVVNIHKNTKKGVVFVTHNIEEAMEIAKKIIVFSKNKRIKQFSVED
                     EYNRDLTKNYYINLKKEILRELGEF"
     gene            complement(2466..3236)
                     /locus_tag="PC414_002976"
     CDS             complement(2466..3236)
                     /locus_tag="PC414_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2465357.1"
                     /translation="MFEKIHKRLKGYYIFLIILILWKIVCVLGIWSSYILPPPEIVLD
                     TFIKMIYDGSIFLNVYASVRRILIGFLISSLMAIPLGVLFGANKKMYEYFKPLFEFMR
                     STPPLALVPMLILWFGIGEESKIIIIILASFFPIFLNTLKGIKNCDNKLIEVGKSFNL
                     SKKRIFYKIIIPNSMLDIVVGLSLGLGYSFRAIIGAELIAASSGLGYLISDGKDMSRT
                     DVVLVGIFVIGFLGILSDYLFSKIITKFSKGKQVDLNG"
     gene            complement(3321..4031)
                     /locus_tag="PC414_002977"
     CDS             complement(3321..4031)
                     /locus_tag="PC414_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HDF2465358.1"
                     /translation="MFKFDVTNELMCIARGSGKFFAKKGAMVAFKGNFNFEKLLLGPS
                     NGGGLGRALLGHVQRSLTGEQMPIMVVEGEGEIYLAQNAYHVDVISIDPGDSIYVESE
                     NLLAFSEQLNYSVKLIGSGVISQKGLFTTHLVNKTGQNQDVAIITDGNPLILEGPCCV
                     DPDALVAWTGREPYPKVSKLSWKTFIGQTSGESYHMEFVEPGQIVIIQPSERLSGLKT
                     DSTPSDRMSSGGLRLGID"
     gene            complement(4211..4363)
                     /locus_tag="PC414_002978"
     CDS             complement(4211..4363)
                     /locus_tag="PC414_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HDF2465359.1"
                     /translation="MATFIIAAIVVVLMALAVMYMVKNSKKNGSSCGCGCSGCSSSKS
                     CHSAKR"
     gene            complement(4389..6545)
                     /gene="feoB"
                     /locus_tag="PC414_002979"
     CDS             complement(4389..6545)
                     /gene="feoB"
                     /locus_tag="PC414_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HDF2465360.1"
                     /translation="MSIKIGLIGNPNCGKTTMFNGLTGSSQYVGNWPGVTVEKKGGKL
                     KGNKDVEIVDLPGIYSLSPYTLEEVVTRNFMLDDKPDAVINIVDASNIERNLYLTTQV
                     LELGIPTVIALNMMDIVNKNGDKINIKELSEVIGCPVVEVTAVKGQGIMEAAEKAVEL
                     ASSNNKLNFKLPFVDESKDAIEKIEKIIEEKTPYIDVETRWLAIKLFERDENVIQKLD
                     ISKTILNSIEEITRNCEDELDDDSESIITANRYEFISSIISSIIKKNRKGKETVSDKI
                     DKIVTNRILALPIFALIMWGVYYIAVSSLGTIATDWTNDVLFGEIIQGNVSNFLASLN
                     VAEWLQGLVVDGLIGGVGAVLGFVPQIMLLFLLLSILEDCGYMSRVAFIMDRIFRKFG
                     LSGKSFIPMLISSGCGVPGVMSTRTIENDRDRKMTIMLTTFIPCGAKIPIIALFAGAL
                     FGGASWVAPSMYFLGIAMIIICGIILKKTSLFAGEPSPFVMELPQYHIPSAKGVLIHM
                     WDRGKAFIIKAGTIIFVACGVIWFLQSFNWSLQMVDAGDSILASLGNIVAPIFAPLGF
                     GNWQSSVATVTGLVAKENVVGTFGVLFGISDATEQDPTLLASVASMFTVASAFAFMAF
                     NMLCAPCFAAIGAIKREMGSWKWTWITLGFQTLTAYIIALLINQVGSLVLGTGGSIAG
                     AIISIFIAVAVVFVVLTYSNKNMKKEKMGKLSYMKN"
     gene            complement(6645..6866)
                     /locus_tag="PC414_002980"
     CDS             complement(6645..6866)
                     /locus_tag="PC414_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDF2465361.1"
                     /translation="MNRLKDIKCGETVKVKKLEGEGATRRRIMDMGITRGVNIFIRKV
                     APLGDPIEVTVRDYELSIRKSDAEKIIVE"
     gene            complement(6879..7091)
                     /locus_tag="PC414_002981"
     CDS             complement(6879..7091)
                     /locus_tag="PC414_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDF2465362.1"
                     /translation="MMPLILSNTGEEVVIKKISGSDKTRTFLNSIGFVVGTNVKIVSK
                     IDGNLIIDVKDTRVALDKKMASRIII"
     gene            complement(7412..10309)
                     /locus_tag="PC414_002982"
     CDS             complement(7412..10309)
                     /locus_tag="PC414_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2465363.1"
                     /translation="MKAFKKHFLILSLIFLLIISCFNNILCFADESENSKKIRVGYCD
                     DYGIISSSKEHSYTGYGYDYLREISKYTRWEYEFVKGSWEECLDRLEKGEIDLLGPLQ
                     KNDERNKLFNFPKLSSGYEYSALYTKDSNNNMFYEDISSFKGMRVAVLRGNFHNTAFE
                     KYREENNFSVEYIYCNSIDELIESVNEKKADAFVCGSIINAKGMKIVSKFSVEPFYFA
                     TAKDKPNLVKELDYALKELKINDMYYELELYKKYFKREVNNSIAFTRQEMNFIKANPK
                     LTMVYDSEWSPVEYYDEESNSFKGISSDLMSIISKKCGIKFEYIKTKNYNESLDYIKS
                     GKATMLCGSINENDKVKRFNMKLTNPYINIPMIMVGKLDTNLNNDLNIALTSSYKSID
                     SYIEKSFENAKTTSYKSVESCLNAINEGKANLMILSSYQFDELLREGKSENLSVISVL
                     DTSYGMRIGVSNKTDPILVSILNKSIDKITEEELSDCIYSNTIDKPYKVPFGVIFKEY
                     SIQIISFVCILFLIAIKYIIYNKKKKEDYLRKIAYTDPLTGADSIDKFKINSNKLFAK
                     NNPEEYALFYIDVDKFKYINDMFGYDMGNDTLIHISNTIASELKEDEIFARVSADHFV
                     LLIKYKTDDDIKTRLNNIYNKVQILSNPKINYYKLILDCGIYKISKSDNDINTIMDRA
                     NTARKTIKGGHKNSFAFYDKEMHKKILKEKEIENSMVDALNNGEFIVYFQPKYSLSDY
                     QIIGAEALVRWDNPQKGLIPPIEFIPVFERNGFIVNIDFYVFEEVCKKIREWMDEGQK
                     VVPISVNLSRMHFVNSNFIEEFKLIVDKYKIPTRLIELELTETAVLDNIEGLLDTMNN
                     LKEKGFVISMDDFGTGYSSLNLLKELPVDILKLDRAFFTEKDESNNEKIVISNVIKMA
                     KELKMKVISEGVETISQVEFLKQIGCDMVQGYLFSKPMPVKEFEKIAFKKE"
     gene            complement(10636..11244)
                     /locus_tag="PC414_002983"
     CDS             complement(10636..11244)
                     /locus_tag="PC414_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HDF2465364.1"
                     /translation="MVDNLIKYIHKSDVAIVVLHEIYGINSFIEDVCQKYYKYGYDVF
                     CPELLGEKRVFPYSKVQEAYDFFINNVGFDIYKKVEKLINDLKSEYKYVFVAGYSVGA
                     TIAWRCSEDSFCDGIICCYGSRIRDYMDVIPKCPTLLVFAKHDSFDVDSISYQLSRKQ
                     NIQIEILDANHGFIDRYSNNYSDIQTKVFTIKEKSFLSKYLA"
     gene            complement(11504..12049)
                     /locus_tag="PC414_002984"
     CDS             complement(11504..12049)
                     /locus_tag="PC414_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HDF2465365.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEKNWADE
                     HRIGVAQGVDEEIIEGLRANFVGECTEVGMYLAMSRQADREGYPEVGEAYKRIALEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKKAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRYFGK"
     gene            complement(12167..13990)
                     /locus_tag="PC414_002985"
     CDS             complement(12167..13990)
                     /locus_tag="PC414_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2465366.1"
                     /translation="MNLSTGNQKVSRLKKKEKPKDFKKTFKRIFMYFKKDKKITRFIF
                     LLVIIDSIISTVIPYTIGKVVDIIDIDTYSKSIFLKGIIILLSFYIIEFSVKLSKGIC
                     TARLSQSIVKEMRSNLFLKFKNLPITFFDKSSTGDIMSRVTNDVDNISTGISNSLVQL
                     ITGILNILMTFIMMFVLSPILTFCSVILIPIVMFVSNFIAKRTRVFFKQQQVELGKLN
                     AHIEEMISNINVVKSFNYERKSVNDFKEVNDRILNISLKSQIYTSLLMPIMNVISNIG
                     FATICIIGGILASKDIITIGVIASFLSYVRQFTRPLNELANLFNTLLSAIAGCERVFE
                     IIDEQEEQEVHNMFKSEINDNKDGNLNSHFVKGEIELKDVSFSYDSKKKILDNINLKI
                     KAGTSNAIIGETGSGKTTIINLITNFYHIDEGSILLDGKDIYSINRNYLRTCFGVVPQ
                     EGYIFNDTVMENIRYGNLEATDEEVIYASKIANAHKFIVKMREGYSTVLSDRGEDLSE
                     GQKQLISIARAVLKNAPILILDEATSNIDIATEAKIQEAINNLTYGKTSIIIAHRLST
                     ISNCDNIIVLENGRIVESGNHKELIKLKGHYYRNILLTQGK"
     gene            complement(14003..15778)
                     /locus_tag="PC414_002986"
     CDS             complement(14003..15778)
                     /locus_tag="PC414_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2465367.1"
                     /translation="MRYFKKYIKKYIVLFLTAVFFLTLEAFCDLAQPTIMAKIIDIGV
                     SNKNLDYILSTGAIMIGITFLGAIFAIIRSVISARVSQHFSYDLRQDVFEKINTYSLK
                     QIDKFGRASLITRITNDINQMQQFVNGMMRVFIKSPIMCIGSLIIAIKLNLKMSIIIL
                     IAVLIIFIIILLNMKIGYRLFKNVQESTDKVNSKLRQFLSGIKVVKLFCRYDYENQSF
                     EELNDELFKNSRKATTMMSFFRPTITIIINVSIIIILLIGRHLIYSGEIKIGIIVAYV
                     NYMTRILTSLIMISHVFNVFVRAKASYERISEVLVDENEEVIEREESLDVGEKDLKFV
                     NCDIIFENVDFSYNKNTDEKILKNVSFKIEEGQNIGVIGQTGSGKTSLVNLIQKLYII
                     DKGDIKIGGRSIYSISEESLRARIGVVFQNSSVFGKSILENVVMGNRDICKEDVVKSC
                     EIANANQFITKLENTYDEVLFQRGKNLSGGQRQRITIARAIAKKPQILILDDCFSAMD
                     INTETIIRKNIKEYISNLREKESKKCTVITISQKISSIIDCDKILVLDEGEVVGFATH
                     EDLLVSSPIYKKIFALQNLVSEDVN"
BASE COUNT         5288 a         2845 c         1566 g         6234 t
ORIGIN      
        1 atagtataat ctaattactt gatattaatt ttcatttgaa ggaggttatc ttttatgtct
       61 tgttgtaatt cacaattttg ttgtaaaaat aaaattgaat catcatatat aaaatggctt
      121 ttagaagttt taggtccagt ctttttaggc tctaaacctg ctgaaataat aaatatttcg
      181 tttactgatg taaatagaga ggaaaaaatt aatgatattt ataaatatct atataagtgt
      241 aagaaaatag attttatagt tattgataaa gaaaaaaagg gattaaaaat cttatttgta
      301 aataaaaaag cattatcaga aaaattaaaa tgtaaaaaaa cagtaaactt tttaaagttt
      361 ttaggataca aacaaaatac gaatgttaat gcttatttag agcatttagt agataaactt
      421 aagagtgatg tttttcctga tgaaataggt atttttttag gatatcctct taaagatgtg
      481 attggattta tgggatatag taattatgaa gtttctatga ttaaatattg gaaagtttat
      541 ggtgacacaa aacaatccga ggacacatat tcgaagtttt tacttcaccg taagaaaatg
      601 agaaaattac tagactacat aagtgtagat aaaatagtaa gctgttttta attgtgtttt
      661 tgtataataa taaaaataca ccctctttta ccatacagtt tccataataa ataagggtgt
      721 attttccact ttaatatttt aaagtattct aattttgatt gtacaacata tctttaatat
      781 ttattttctt ttgttgcatt ccattagaca tcaaaaattc ctgtgtatct tctaattctt
      841 ttatatcctt gtccgttatt ttagaactaa agtcatacca agaatacatt tcttttgttt
      901 catctaaaga taaaccaact tctttagata caacatccat cacttcatcc ttgttttcat
      961 tcatatattt taaagtttct tcatgaacct tcataaatct ttttactaat tcaggatatt
     1021 tttcagcaaa gtcatcactt acagcagtaa ctattattcc acttactaat ccttctccat
     1081 ttacaatcat tttggcacct gaatttatcg ttttcaatgc cactggacct gcaagcattg
     1141 ctacatcagc acttttactt tctaatgcag aagcagcact aggaatatcc atatttacat
     1201 attcaacatc atctatagta tagccttctt tagcaagtgc agcaatcaac agttgatgaa
     1261 gtatagtccc ttttggacct acaatttttt taccttttaa gtcttttata gatttaatat
     1321 tatcattatt agttataagc atatatcctt ttggtgaacg gctataaata ttcgtaattt
     1381 taagaccaac accatttgat gcagctatta ttgcagacgt tcctcctaat gcatgtaaaa
     1441 agtctagctc acctgctgct aatgcttgag tttgctctgg tcctgttgtc aattcatgga
     1501 aattgacttt tatattgtcc tttttaaatt cctttccaaa taaatcatcc tttttttcta
     1561 atatactagg aacattaagt ggtgatttta cataagttaa atttatttcc ttaggtaaac
     1621 tagcctcttt ttttgcaggt tcatctttct tatcatttgt tgttgaacat cctacagtcc
     1681 caaccaacac cattattaaa gatgtcatta ctgctaaaat ctttttgttt cttttaatca
     1741 attaaaactc ccccaattcc cttaatattt cttttttcaa gtttatataa tagtttttag
     1801 ttaaatctct attatattca tcttcgacac taaattgttt aatcctttta tttttactaa
     1861 aaacaattat tttctttgct atttccatag cttcttcaat attgtgagtt acaaatacta
     1921 cacctttttt agtattttta tggatattta caacttcttt ttgcattttt cttcttgtga
     1981 agtaatctag tgctgcaaat ggttcgtcca tcagcaaaat atcgggattg aaggaaagtg
     2041 ctcttgctat acttactctg tgagccattc ctcctgataa ttcatttgga tatgagtttt
     2101 taaacttttc aagcttcatc atttttaaat acttgtctaa atcaacttta tttctattat
     2161 ctttttcagt atgtaaaagt atattttcac ttacattcag ccatggcata agtctacttt
     2221 cttgaaatac cattcctact tttggagtac actttttatc attattaaaa aaatatacgt
     2281 ttccactagt agcattttca agccctgcta aaattctaag tagcgttgtt ttcccacatc
     2341 cactttcacc taatataaca gttatctctt cactagatat atttaaagat atattatcaa
     2401 gaactaaatg ctctttatta tcaactaaat atttcttata tatattctct agcaaaaatc
     2461 cacttctaac catttaaatc aacctgcttc cctttgctaa attttgttat tatctttgaa
     2521 aatagataat ctgaaagtat acctaaaaat ccaataacaa aaattcctac caatacaaca
     2581 tcagttctag acatatcttt tccatcagaa atcaaatatc ctagtcctga agaagctgca
     2641 ataagctctg ctccaattat agccctaaaa ctatacccaa gccctaaact aagtccaaca
     2701 acaatatcca gcatagaatt agggattatt attttataaa aaattctctt tttagataaa
     2761 ttaaaagatt tacctacttc tattaattta ttgtcacaat tttttatacc tttcaatgta
     2821 tttaaaaata ttggaaaaaa ggatgccaaa ataataatta taattttaga ttcttcacct
     2881 ataccaaacc ataaaataag cattggcacc aaagctagtg gaggtgtact tcgcataaat
     2941 tcaaatagtg gtttgaaata ttcatacatt tttttatttg ctccaaataa aactccaagt
     3001 ggtatagcca tcaatgaact tattaaaaaa cctattaata tccttcttac actggcataa
     3061 acatttaaga aaatagaacc gtcatatatc atttttataa aagtatctaa gactatttca
     3121 ggaggaggca gaatatagct actccatata cccaaaacac atactatctt ccataaaatt
     3181 aaaattatta aaaaaatata atatccttta agccttttat gaattttctc aaacatataa
     3241 aactcctttt aacatatcta tccctgctac cctttttatt ttttaatatc ttaaataata
     3301 attaagctat atcataagat ttaatctatg ccaagtctta atccacctga actcattcta
     3361 tcagaaggtg tagaatctgt ttttaagcca gacaatcttt cagatggttg tataatgact
     3421 atttgtccag gttcaacaaa ttccatatga taagattctc ctgaagtctg accaataaaa
     3481 gtcttccatg atagttttga tactttagga tatggttctc ttccagtcca agctactaat
     3541 gcatctgggt caacacaaca aggtccttca agaattagag gattaccatc tgttattata
     3601 gcaacatctt gattttgacc tgttttattt actaaatgtg tagtaaaaag acctttttga
     3661 gatattaccc ctgaaccaat caattttaca ctgtaattta attgttcaga aaatgccaat
     3721 aagttttcac tctcaacata aatggaatca cctgggtcaa ttgatattac atctacatgg
     3781 taagcatttt gagctaggta aatttcacct tcaccttcaa ccaccattat tggcatttgt
     3841 tctcctgtca atgacctctg aacatgacct aataatgctc ttcccaatcc accgccatta
     3901 cttggaccta acagcaattt ttcaaaatta aaatttcctt taaaggctac catagcacct
     3961 ttttttgcaa aaaatttacc acttcccctt gctatacaca taagctcgtt tgtaacatca
     4021 aatttaaaca taatattcac ttatattgct taatttagca ataatccctc ctaatcatat
     4081 atttatatat aattatacag taaaattaca gcatagtcta acctacttca aatatgaaat
     4141 ttgttattta tacaaaaaat aagaacctga taataaagca atttaacttt ttatcaggct
     4201 cttatcctat ttatctctta gcgctatgac aactcttact acttgagcat ccagaacaac
     4261 cacatccaca actgctacca ttcttttttg aatttttaac catatacatt acagctagtg
     4321 ccattaatac tactacaata gctgctataa taaatgtagc catattcatc actccttatt
     4381 tatatgaatt aatttttcat ataacttaat ttacccattt tttctttttt catattttta
     4441 tttgaataag ttaaaactac aaagactact gctactgcta taaatataga tattattgcc
     4501 ccagcaatac ttcctccagt acctaaaact aaacttccaa cttgatttat aagaagagca
     4561 attatatacg ctgttaaagt ttggaatcct aatgtaatcc atgtccattt ccatgaaccc
     4621 atttctcttt ttatggctcc aatagctgca aaacaaggtg cacaaagcat attaaatgcc
     4681 ataaatgcaa acgcactagc tactgtaaac atacttgcaa ctgaagcaag aagtgttggg
     4741 tcttgctctg ttgcatctga tataccaaat aaaactccaa atgttccaac tacattttcc
     4801 ttagctacta gacctgtaac tgttgctact gatgattgcc aatttccaaa tccaagagga
     4861 gcaaatattg gtgcaactat atttcctaag ctagctaata tactatctcc agcatctacc
     4921 atttgtaatg accaattaaa tgattgtaag aaccagatta ctccacaagc aacaaatata
     4981 attgttcctg ctttgattat aaatgctttt cctctatccc acatatgtat taaaacacct
     5041 tttgcacttg gaatatgata ttgaggtaat tccataacaa atggagatgg ttctcctgca
     5101 aataaactag ttttctttaa tataattcca caaatgatta tcattgcaat acctaagaaa
     5161 tacattgatg gagctaccca tgaagcacct ccaaataatg cccctgcaaa taatgctatt
     5221 attggaatct tagctccaca tggaatgaat gtagttaaca taatagtcat ttttctatct
     5281 ctatcattct ctatagtacg agttgacata actcctggaa ctccacaacc tgaacttata
     5341 agcattggta taaatgattt tcctgaaaga ccaaacttac ggaaaattct atccattata
     5401 aaagctacac gtgacatgta cccacaatct tctaatattg ataataataa gaataaaagc
     5461 atgatttgtg gtacaaatcc tagtactgct ccaactccac caattagacc atcaactaca
     5521 agtccttgta accactctgc tacatttaaa gaagctagaa aattacttac attgccttga
     5581 ataatttctc caaacagtac atcatttgtc caatcagttg ctatagtacc caatgaactt
     5641 actgctatat agtaaactcc ccacattata agtgcaaaaa taggtaatgc tagtatacga
     5701 tttgttacaa tcttatctat cttatctgat acagtttcct taccttttcg attcttcttt
     5761 attatacttg aaattattga actaataaat tcataacgat tggctgttat tatactttca
     5821 ctatcatcat ccaattcatc ttcacaattt ctagttattt cttcaatact attcaatatt
     5881 gttttagaaa tatcaagttt ttgtataaca ttttcatctc tctcaaatag ctttatagct
     5941 aaccagcgtg tttctacatc tatatatgga gtcttttctt ctattatttt ttcaattttt
     6001 tctattgcat ccttactttc atcaacaaag ggtaatttga aatttaattt attgttacta
     6061 cttgcaagtt caactgcctt ttcagcagcc tccataattc cttgaccttt aactgctgtt
     6121 acttctacca ctgggcaacc tataacttca gatagttctt taatatttat cttatcacca
     6181 ttcttattta caatatccat catattaaga gctataacgg tcggtatacc tagctctaaa
     6241 acttgagttg taagatataa atttctctct atatttgatg catctacaat atttattact
     6301 gcatccggct tatcatctag cataaaatta cgtgtaacta cttcttctaa tgtatatgga
     6361 gataaagaat aaatacctgg taaatctact atttccacat ctttatttcc ttttaatttt
     6421 ccaccttttt tttcgactgt tactcctggc cagttaccaa catattgtga tgagcctgta
     6481 agcccattga acattgttgt cttaccacag ttaggatttc ctatgagacc tatttttatt
     6541 gacatttcca ttccctccat tattatttaa atacatcact gtttgtgata cagaaaatca
     6601 ttctcattat ataattacat taaccctact ctaattaatc tttattattc cacaataatt
     6661 ttttctgcat ctgacttacg aattgatagt tcataatctc tgactgttac ctcaattggg
     6721 tcaccaagag gagctacttt tcttataaat atattaacac ctcttgtaat ccccatatcc
     6781 attatacgtc ttcgtgtagc tccttctccc tctaactttt tgacttttac agtctctcca
     6841 cacttaatat ccttcagtct gttcatgtca ttacctccct atattattat tctacttgcc
     6901 atttttttat ctaatgcaac tctagtatct tttacgtcta ttattaagtt accatctatc
     6961 ttagaaacta ttttaacatt tgtacccact acaaaaccta tactatttaa aaaagtcctc
     7021 gtcttatcac ttcccgaaat tttctttata actacttctt cacctgtatt tgataatatc
     7081 aatggcatca tataatatcc ctcctcttaa agtaatttaa atctacaagc aatcgttatc
     7141 ttatatataa attacaatac cttaaatctt aatctattaa aataacatcc tattttttcc
     7201 ttctttttac aattcctaat tttgatttaa gtctatcaca atagtatatt ttttgcaagt
     7261 gattattgta ttcttttgat tttttttctc atttgattta ttttattaat aaaatcgtaa
     7321 aaattaatac aaaaataaag atgctctaaa atagtatact caactaatat taaagcacct
     7381 ttattatata tgtcattatt tacacattgc tttattcttt tttaaaagcg attttttcaa
     7441 actcttttac aggcattggc ttagaaaaca aatatccttg taccatatca cacccaattt
     7501 gctttagaaa ttctacttgc gaaatagtct ctacaccttc tgatattact ttcattttca
     7561 gttcctttgc cattttaatg acattagata taacaatttt ttcattatta ctctcatctt
     7621 tttctgtaaa aaatgctcta tctagtttta atatgtcaac aggcaattct tttaaaagat
     7681 taagagagga atatcctgta ccaaaatcgt ccatagagat tacaaatccc ttttctttca
     7741 aattgttcat tgtatcaagt aatccttcta tattatctaa tactgctgtt tctgttagct
     7801 ctaattctat aagtcttgtc ggaattttgt atttatctac aattaattta aactcctcta
     7861 taaaattact atttacaaaa tgcattcgag acaagtttac agatataggt actacttttt
     7921 gtccttcatc catccattct ctaatctttt tacacacttc ctcaaataca tagaaatcaa
     7981 tatttacaat aaaaccattc ctctcaaata caggaataaa ttctattggt ggtattagac
     8041 ctttttgtgg attatcccaa cgaacaagag cctctgcccc tataatttga taatctgaaa
     8101 ggctatattt aggttgaaaa taaacaataa attctccatt attaagagca tctaccattg
     8161 aattttcaat ttctttctct tttaatattt ttttgtgcat ttccttatcg taaaaagcaa
     8221 atgaattttt atgaccacct tttatagttt ttcttgcagt attagctcta tccataatag
     8281 tatttatgtc attatcactt ttacttattt tatatatacc acaatctaaa atcaatttat
     8341 aatagtttat tttaggatta ctaagtattt gaaccttatt gtaaatattg tttaaccttg
     8401 ttttaatatc atcatctgtc ttatacttaa taagaagaac aaaatggtca gcagaaactc
     8461 ttgcaaaaat ttcatcttcc tttaattcac ttgcaatagt atttgatata tgtatcaaag
     8521 tatcatttcc catatcatat ccaaacatat catttatata tttaaatttg tcaacatcta
     8581 tataaaacaa cgcatattct tctggattat tcttagcaaa cagtttatta gaatttattt
     8641 taaatttatc tatactatct gctcctgtca atgggtctgt atatgctatc ttccttagat
     8701 aatcctcttt cttcttttta ttataaatta tatatttaat agcaattaag aataaaatac
     8761 atacaaaaga aataatttgt attgaatatt ctttaaaaat cacaccaaat ggtactttat
     8821 aaggtttgtc aatagtatta gaatatatac aatcacttaa ttcttcttca gtaattttgt
     8881 ctattgattt atttaaaata gacactaata tagggtcagt tttatttgat acccctatac
     8941 gcatcccata gcttgtatct aaaacagata taacagataa attctcactc tttccttctc
     9001 ttaaaagttc atcaaactga taagaactaa gtatcattaa gtttgcttta ccttcattta
     9061 tagcgtttag acaactttct acactcttat aagatgttgt cttagcattt tcaaaactct
     9121 tttctatata agaatcaata gacttataac tactagttaa agcaatattt aaatcattat
     9181 tgagattggt atccaatttt cctaccataa tcattggaat atttatgtat ggatttgtca
     9241 atttcatatt aaatcgtttt accttatcat tttcattgat tgagccacat agcatagttg
     9301 ccttaccact ttttatgtaa tcaagactct cattataatt ttttgtttta atatattcaa
     9361 attttatacc acattttttt gatataattg acattaaatc ggaacttata cctttaaatg
     9421 aattgctttc ttcatcataa tactctacag gagaccactc tgaatcatat accatagtaa
     9481 gttttgggtt tgctttaata aaattcattt cttgccgagt aaatgctatg gaattattga
     9541 cctctctttt gaaatatttt ttatataatt ccaactcata atacatatca tttattttta
     9601 attcttttaa agcataatct aattctttta ctaaattagg tttatctttt gcagtagcaa
     9661 aataaaaagg ttctactgaa aatttgctaa ctattttcat tcctttagca tttataatac
     9721 ttccacaaac aaatgcatct gccttttttt cattgacgga ctcaattaat tcatcaatag
     9781 aattacagta tatatattca actgaaaaat tattttcttc tctatacttc tcaaatgcag
     9841 tattatgaaa gtttcctcta agtacagcta ctctcattcc cttaaaggaa cttatatctt
     9901 cataaaacat attattgtta ctatcctttg tatatagtgc actatattca tatcctgaac
     9961 ttagtttagg aaaattaaat agtttatttc tttcatcatt cttctgtaaa ggtccgagta
    10021 agtctatttc acccttttct agtctgtcta aacattcttc ccaagaccct tttacaaatt
    10081 catattccca tcttgtatat tttgagattt ctcttagata atcataacca tatcctgtat
    10141 aagaatgctc ttttgatgag gatattattc cgtagtcatc acaatatcca actcttattt
    10201 tttttgaatt ttcagactca tcagcaaagc atagtatatt gttaaaacat gatataataa
    10261 gtaaaaatat caaagaaagt atcaagaagt gctttttaaa tgctttcata tttaaaaacc
    10321 tccaatttgc caacttactt taacttattt cgttttctag ttgtaactac ttttattgta
    10381 atggtaaata tatcaaattg caatattctc atataatatt cttattcaac tataatgaat
    10441 ttattattat ctatttaaat acactatcta atattagttt cttataagtc ataaaagcac
    10501 aactaacact ataattctag tgattaattt tatttcaaaa aatataataa cactactaat
    10561 attttaaata tcaatagtgt cataaaaact tttcagatta attaaatctt tctatatatt
    10621 ttataataat ctaaatcatg ctaaatattt agacagaaaa cttttttctt taatagtaaa
    10681 tacttttgtt tgaatatcag aataattatt tgaatatcta tctataaaac catgatttgc
    10741 atctaaaatc tcaatttgga tattttgttt cctagaaagt tggtaagaaa tagaatcaac
    10801 atcaaatgaa tcgtgctttg caaatactaa taaggtagga cattttggaa ttacatccat
    10861 gtaatctcta atccgagagc cataacaaca aattatgcca tcacaaaaag aatcttcact
    10921 gcatctccat gcaatagttg ctcccacgct atacccagca acaaatacat atttatactc
    10981 tgattttaaa tcatttataa gcttttcaac ttttttataa atatcaaaac caacattatt
    11041 tataaaaaaa tcatatgcct cttggacttt tgaatatgga aaaactcttt tttctcccag
    11101 aagttcagga caaaatacat catatccata tttataatac ttttgacaaa catcttctat
    11161 aaagctatta atcccataga tttcatgcaa aacaacaata gctacatcac ttttatgtat
    11221 atactttatc agattatcca ccacctttac aaaacagcaa attttttata cttagtttaa
    11281 tatattcttc tttacatgtc aatttacttt tctaaaatca aatttatttt tggattaatc
    11341 atttattttt aaaagttgtg cattattact attaaattat tctctattat taaaaatata
    11401 atcgttagaa ataaaaaata aagtacaaat aattaaaaaa tatctcactc ataactttaa
    11461 aaattataag tgagatactc ctaaaatcta ctttatatta aatttatttt ccaaaatatc
    11521 tatttaataa tcctaaaaat gctttaccat gtctagcttc atctttacac atttcatgta
    11581 ctgtatcatg tatagcatct aatcctaatt cttttgcttt cttagctaat tttaattttc
    11641 catcagttgc accatactca gcatcaactc taactcttaa gttttctttt gtatctgcaa
    11701 ctacaacttc tccaagaagt tctgcaaatt tagcagcatg ttcagcttct tcaagagcta
    11761 ttctcttata agcttctcct acttctggat aaccttctct atcagcttgt ctactcattg
    11821 ctaaatacat tcctacttct gtacactcac caacaaagtt agctcttaat ccttcgatta
    11881 tttcttcatc tacaccttga gctactccta ttctgtgctc atcagcccaa tttttttcgc
    11941 ctttcatttc ttcaaattta tcagctccaa ctttacatac tggacattgt gctggtgcag
    12001 catctccttc atgtatatat ccacatactg tacaaacaaa tttcttcata attactttcc
    12061 tcctaaattt tatatatcaa attaaatttt ttatttatta agttagtcta tatatacccg
    12121 caccatatac ctttaaacaa tttttttaaa agttttttct aaatacttat tttccctgtg
    12181 tcaataatat gtttctataa taatgtccct ttaattttat caattcttta tgatttcctg
    12241 attccacaat tctaccattt tctaaaacta ttatattatc acaatttgat atggtactta
    12301 atctatgagc tataattata cttgtctttc cataagttaa attatttatt gcttcttgaa
    12361 ttttagcttc tgttgctata tctatgttac ttgtagcctc atccaaaata agtattggag
    12421 cattttttaa tactgctcta gcaatactta ttaattgttt ttgaccttca cttaaatctt
    12481 cacccctatc cgataataca gtactatatc cttcacgcat tttaactata aatttatgtg
    12541 catttgctat tttactagca tatataactt cttcatcagt agcctccaga tttccatacc
    12601 ttatattttc cataactgta tcattgaata tataaccttc ttgtggaact actccaaaac
    12661 aagttctcaa atagtttctg ttaatagagt atatatcctt accatctaaa agtatacttc
    12721 cttcatctat atgataaaaa tttgtaatta gatttataat agttgttttt ccacttcccg
    12781 tctctcctat gatggcattt gatgtgccag ctttgatttt taggtttata ttatctaaaa
    12841 ttttcttttt tgaatcataa gaaaaactta catctttaag ctcaatttct ccctttacaa
    12901 aatgagagtt taaattacca tctttattat cgttaatctc acttttaaac atgttatgca
    12961 cttcttgctc ttcttgttca tctataattt caaaaactct ttcacatcca gctatggctg
    13021 aaagtaatgt attaaataga tttgctaatt catttaaagg tcttgtaaat tgtcttacat
    13081 aacttaaaaa actagctata acccctatgg ttataatatc tttagatgca agaatgcctc
    13141 ctataataca tatagttgca aatcctatat tactaatcac attcataata ggcattaaaa
    13201 gagaagtgta aatttgcgat ttaagggaaa tatttagaat tctatcattg acttctttaa
    13261 aatcatttac agattttctt tcataattaa aagattttac tacatttatg tttgatatca
    13321 tttcttcaat atgtgcattt agttttccca actcaacttg ttgttgctta aaaaaaactc
    13381 tagttctctt tgcaataaaa ttactcacaa acattactat cggtattaat attacactgc
    13441 aaaaggttaa aattgggctt aatacaaaca tcataataaa agtcatcaat atgtttaaga
    13501 ttcctgtaat taactgtact aatgaatttg atattcctgt actgatatta tcaacatcat
    13561 ttgtcactct actcataata tctccagtag aacttttatc aaaaaaggtt attggtagat
    13621 ttttaaattt taaaaataaa ttacttctca tttctttaac tatactttga gataatctag
    13681 ctgtacatat ccccttagac agtttaactg aaaactcaat tatataaaat gaaagtaaaa
    13741 taattattcc ttttaagaaa atggattttg aataagtatc gatatctatt atgtcaacta
    13801 cctttccaat agtatatggt attacagttg atataattga atcaattatt actagcaaaa
    13861 aaataaatct agtaatcttc ttatcttttt taaagtacat aaaaattctt ttaaacgttt
    13921 ttttaaaatc tttaggcttt tcttttttct ttaatctact gaccttctga tttccagtac
    13981 ttaaattcaa tatacttcct ccttaattta catcttcact tactaaattc tgcaacgcaa
    14041 aaattttttt ataaattgga gaactaacca gtaaatcttc atgtgtagca aatccaacca
    14101 cctcaccctc atccaagact aaaatcttat cacagtcaat tatagaacta atcttttgtg
    14161 aaatagttat tacagtacat ttcttacttt ccttttctct taaattactt atatactctt
    14221 ttatattctt tcttataatt gtctctgtat taatatccat tgcactaaaa caatcatcaa
    14281 gaattaatat ttgtggtttt ttggctatag ctctagctat agtaattctt tgtctctgtc
    14341 ctcctgataa atttttaccc ctttggaata aaacttcatc ataagtattt tcgagttttg
    14401 ttataaattg atttgcattt gctatttcac atgattttac aacatcctct ttgcatatat
    14461 ctctatttcc cataactaca ttctccaata tactttttcc aaacacacta ctattttgaa
    14521 atactacacc tattcttgcc cttaaacttt cttcagaaat actgtatata ctacgtcctc
    14581 caatcttaat atcaccttta tcaattatat ataatttttg aattaaattt actaaagaag
    14641 tctttcctga accagtttga ccaatcacac caatattttg accttcttct attttaaagg
    14701 atacgttttt taatattttt tcatctgtat ttttgttata cgaaaaatct acattttcaa
    14761 aaattatatc acagtttaca aactttaaat ctttttctcc aacatctaaa ctttcttctc
    14821 tctcaattac ttcttcattc tcatcaacca aaacctcact tattctctca taagatgctt
    14881 ttgccctaac aaaaacatta aaaacatgtg aaatcattat aagagatgtt aatattctag
    14941 tcatatagtt tacataagca actataatcc caattttaat ttctcctgaa taaattaaat
    15001 gccttccaat tagtaatatt attattatgg aaacattaat gattattgta attgtcggtc
    15061 taaaaaatga catcatagtt gtagcttttc tactattttt aaaaagttca tcatttaact
    15121 cttcaaagct ttggttctca taatcgtatc tacaaaatag tttaactact tttattccac
    15181 ttaaaaactg tcttaatttt gaatttactt tatctgtact ttcttgtaca tttttaaata
    15241 atctataccc tatcttcata ttaagcaata ttataataaa tataattaaa actgcaatta
    15301 gaataattat tgacatcttt aaatttaatt ttatagctat aattaaactt cctatacaca
    15361 ttattggtga ctttataaaa actctcatca ttccattaac gaattgttgc atttgattga
    15421 tatcattagt aattcttgtt attaatgaag ctctaccaaa tttatctatt tgttttaatg
    15481 agtatgtatt tatcttttca aatacatcct gtcttaaatc ataagaaaaa tgctgagaaa
    15541 ctcttgccga gattacactt cttattattg caaatattgc acctaaaaaa gttataccta
    15601 tcattatagc tcctgttgat agtatgtagt ctaagttctt atttgaaacc cctatatcta
    15661 ttatttttgc cattatagtt ggttgagcta aatcacaaaa agcttccaat gttaaaaaaa
    15721 atactgctgt tagaaataat actatatatt tttttatgta ttttttaaaa tacctcattc
    15781 aatcatccct tcatagaatt ttctcattac caaaagtata tcataatggc taatttaatt
    15841 gattatatca attataaata tttgatttat tattaatttt tataccatat attttataaa
    15901 ttatttgttt ttatttccta tatttatata tat
//
LOCUS       DAMJVK010000094        15488 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.097, whole genome shotgun sequence.
ACCESSION   DAMJVK010000094 DAMJVK010000000
VERSION     DAMJVK010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15488
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.097"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            51..902
                     /locus_tag="PC414_002987"
     CDS             51..902
                     /locus_tag="PC414_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HDF2465368.1"
                     /translation="MKNIISAKELISKLEKNDNLVVIDCRFDLINRTYGIDSYKKGHI
                     KGSFILDIDKDLSSPTKEHGGKNPLQDPLILKEKLEKMGVDNDTTIVTYDDGDLNGAC
                     RLFFQLKHLGLKNVYVLDGGITSFVKEGGELEEKVNIPSCTGKEIRPNINNDLVVPME
                     YVKSKLYKKDTVIIDCRANERYQGLVEPAYSKAGHIPSAKNYFCKDLIKSDFENGSLK
                     DIEFLKKFFKDLNNYDEVILSCGSGISACVNSLALRELDIPHKIYIGSFSDWISYDDN
                     EIKTGQE"
     gene            complement(1035..2648)
                     /gene="ggt"
                     /locus_tag="PC414_002988"
     CDS             complement(1035..2648)
                     /gene="ggt"
                     /locus_tag="PC414_002988"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HDF2465369.1"
                     /translation="MKFNAYQHKYSSIRNVVYAKNGAVATSTPLASQAGLEILKKGGN
                     AVDAAVATAATLAVVEPTSNGIGGDAYALVWIEEEKRLYGLNSSGFAPENMELKNYNN
                     MKEMPKYGFGAVTVPGIPAAWSELNKKYGKLSLMECLSPAINYAREGYVVSPNVAKVW
                     KKSYELYEKELIGEEFKPWFDTFSKDGKAPEAGDIFICEEQASTLEEIANTQAESFYR
                     GRLADKIDEYSKKFNGAIRKSDLECFYPTWVEPISTEYKGYKIFEIPPNGHGITVLMA
                     LNILKELELEGNIENVEDIHKIIESLKLAFADSKTYVTDIEHMKVKIQELLSQEYAKK
                     RSLLIDNKEALYPTAGEPYCGGTVYLCTADKDGNMVSYIQSNYINFGSGIVIPRTGIA
                     LHSRGNNFNLDPKHHNVVKPFKKPYHTIIPGFLGKEDKAIGPFGVMGAFMQPQGHIQV
                     LTNMIDFGLNPQEALDAPRWQWIKGKEIEVEPEMPKHIIDSLIEKGHEIKVIHDTVDM
                     GRGQIIFKTEQESYICGTESRCDGHIAVY"
     gene            complement(2873..4810)
                     /locus_tag="PC414_002989"
     CDS             complement(2873..4810)
                     /locus_tag="PC414_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2465370.1"
                     /translation="MTLFRIAMQNVKNSFFNYLMYFISIVFSVFVFFSFKSIEYNEAL
                     SSLGEKTRMSINTSSIVIVVFVFLFIYYSNSFFFNRRRREVGTYSLLGMRKNQIGKIF
                     LYETFLMGIVAILIGIFLGLLFSKLMTMMLVKLMNEMIVVNMNLSIKALVQTLAVFLI
                     IFIVIGIRNTIVIRNKRIIELFNKYPEKYKFKKFIKLKCVLGVLLIAISYLMSISYFI
                     AENIMLSVFILVTIIPGTFLFFSSTMYMIIDAVKKRKSFYYKGQNLIAFSELGFKLKS
                     NSSVLAVIAILIATSVTMLGFTISLYYDIDRNISENYKYSYTINLGNSYVNNEIDKLL
                     YKYKKDNKIIFDKTIELIDKDINIEMYYKNGNSYIEDRTSVDIIRESDFKKLRQYQNN
                     NYEELTSKESVYYVSDSYKKIFFKDFEVEKINLHIKDTNEYDINLFKVQKSILEPEIN
                     SQATFDLIVAKDDVFNQLKLHGNSRLIRLIDIKNEKKELDLTLKLKNIVSENMKFTYP
                     FNFTSSIESYNNLIKLSGLMLFIGIFLSVVFLLCTGSIILFKQLSNIYDDKERYIMLI
                     KLGANNKDIEKIISKQLKVIFLMPLVVGTVHNLFAMSIAQKFIPRSLLVPIIITLVIY
                     FIGYFIYYFLTLKYALNMIKE"
     gene            complement(4797..5564)
                     /locus_tag="PC414_002990"
     CDS             complement(4797..5564)
                     /locus_tag="PC414_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003722224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2465371.1"
                     /translation="MEEILSVENIKKEYGRKGSKHEALRGITFKVYKGEFVGIMGSSG
                     AGKSTLLNIISTIDLPSSGDIYINGKNTIKMKQNELADFRRDNLGFVFQDSNLLDTLT
                     IKENIMLPLSLKNERVSVIENRIKEISKELNIESILDKYPGEVSGGQKQRGAVCRAIA
                     TKPSLVLADEPTGALDSKSARDLLNCLLKLNKDSNKTILMVTHDAISASFCNRILFIK
                     DGIIFTEIVKGESNREFYNKIVNTVSLIGGVNKNDFI"
     gene            5852..6526
                     /locus_tag="PC414_002991"
     CDS             5852..6526
                     /locus_tag="PC414_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2465372.1"
                     /translation="MFKIMVVEDDVSLKNIIAKCLTKWGHDVHQIENLENIIEEFKNY
                     NPELVLLDINLPFYDGFHWCNEIRKISKVPIIFISSRNSNMDVIMGVNLGADDYIQKP
                     FSVDVLVAKVNALLRRTYNFVDNNSNQIIHNGVTLDLSTATINYEDNTIELTKNEIKI
                     LHELMKYKGQIVSRNKLMKKLWDNDWFVDDNTLTVNVNRIRSKLNEIGLEDFIETKRG
                     LGYIIS"
     gene            6550..7554
                     /locus_tag="PC414_002992"
     CDS             6550..7554
                     /locus_tag="PC414_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2465373.1"
                     /translation="MSFKDFLKDKLGFLVYNFTFLIFTVSVIIFSPVDVFLTDTILYI
                     LVVNVVFLFLYLFISYIKKNKFLDKIKNDTFKINMNDIELARCKNEEKIYLNIIKNHQ
                     YQCNNIINNNIEKFEENKEILNMWVHDVKIPIAIIKLILEQNENLIDEKISDDIDSEI
                     FRIENSVEKILYLSRLEDFHKDFLIQEVNIEKIIRNIIKKYSKYFISKKIILDLNNLN
                     FKVLSDEKWLYFIFEQLLSNSLKYSSLGDNISIYCKRTKNYLQLRVEDTGCGIKKEDL
                     NRIFNKGFTGNNGRNNSKSTGLGLYLVKELCDKLGHKIDVDSEYNQYTKFTITFKCDL
                     "
     gene            7666..8895
                     /locus_tag="PC414_002993"
     CDS             7666..8895
                     /locus_tag="PC414_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HDF2465374.1"
                     /translation="MSFLKKFKNKRFRNNSFKSNNFRNSSFGSSGFKSKNKGFKNNPF
                     KNKRFKIYAGIAVIILLILGVLAYVDSKNTELQANENFIETYTIPENEKIFINGMVVP
                     KQTKDFNISGDYELSDVNVTNGQKVNQGDLLFTAKNPTIIAEIDSLKSQLSQYKKQKI
                     SLSDIAENKDAIASINAQITALSSQIASLDKKAYDRVTAPFDGTVYLNDQTGNPDQPV
                     SFMTIQGLEFYMKGQASEQDLPKMKIDQMVNILVFSTDQKLTGRISFISDKPSTPNTE
                     MGAQQNTLSYYDINIAFDNQEGLVNGFHLQASLEVANSSFKIPASCVLKDNKHSYVFK
                     DLDGILKKQIVDVASQNDDFAVVRGGLEQGDIIIKHPTKEMKEGDPVQGDGVTAGSNN
                     GDTTPNKKVEEVTMDVN"
     gene            8903..9577
                     /locus_tag="PC414_002994"
     CDS             8903..9577
                     /locus_tag="PC414_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2465375.1"
                     /translation="MLIKLENIQKYYKVGKDELHVLKSLNLEIESGEFVMIMGKSGSG
                     KTTLLNILGFLDVFDEGRYIFDGTDVTNLSENERSVFRNINIGFVFQQFNLIETLNVY
                     QNVELPLIYNKALKKSNREEIVKDKLSSVGLLDKLKQKPLQLSGGQQQRVAIARCLAN
                     DPQIIFADEPTGALDSETSREIMELLTRLNKQGKTIIMVTHDQDLTKYATKVIRLKDG
                     VFTSEV"
     gene            9580..10812
                     /locus_tag="PC414_002995"
     CDS             9580..10812
                     /locus_tag="PC414_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2465376.1"
                     /translation="MSLLKNSLANLKGHKLRVFVALLWIIIGITSVILVSSIGNGFQK
                     EIKKSVNNVNPNKTTISFESADNTGLTDDMSIFLKPFNAKDLEELSFVEGVERIAPSR
                     DGFNLDSVYSSQASFDKKTTYVDVGPVKKDSKINLICGRDFSLDDEKRKVILLTLQST
                     SEIFENPEDALGHGININGTIFEIIGVLDDSQQNQAGGFFGGYQDMQFTTSLVPKKAF
                     DTLMSQNSYSNEIYQLDLVSSKGYNVNEVANNVIAKLYEMHPGINGSYTTPDPTEQTA
                     YLESINSNVNKYVSIITVVAMFVGGIGVMNIMYVSVMERQREIGIRRAIGAKPRSILF
                     QFLVEAVFITVCGGILGTIVGFTATNYVSKYIGFEAIPSLNSLFYAIVATILTGVVFG
                     LIPAFKASKLDPIKAIYK"
     gene            complement(11098..12363)
                     /locus_tag="PC414_002996"
     CDS             complement(11098..12363)
                     /locus_tag="PC414_002996"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HDF2465377.1"
                     /translation="MILIKNGRVIDPKSQRDENIDLIIKENKIYKIGKFDESDEYEKI
                     IDASGNVVAPGLVDVHVHFRDPGFTYKEDIESGAKSAARGGFTTVICMANTNPIVDNE
                     DTFNYVKEKSKNACINVLQAAAITKGFEGKELVDMEALKKAGVPGFTDDGLPLMDSNL
                     IMEAMIKAKELDVPLSFHEEDPSLVGNPGVNAGKVASELGLKGASSVAEDVMVARDCM
                     LALKTGAKVDIQHISSGVSVDMVRFAKYLGANVVAEASPHHFTLTEEDVLEFGTNAKM
                     NPPLRSKWDRDKIIEGLNDGTIEIIATDHAPHSKEEKDREFIKAPSGIIGLETSLALG
                     ITNLVHKNHLSMMQLIEKMSINPAKLYNLNIGFIEEGAVADIVIFNPEEEWTVESFVS
                     KADNSPFKGKSLYGKVNYTICNGEIVYNA"
     gene            complement(12416..13564)
                     /locus_tag="PC414_002997"
     CDS             complement(12416..13564)
                     /locus_tag="PC414_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HDF2465378.1"
                     /translation="MKSVSVRSCKTYNYEEVKLSIEKNLEDIGGIDKFVKKGSKVLIK
                     PNLLMKKKPEEATTTHPMVVKVVCEKLLELNCDITIADSPGGPYNQSSLKSIYKASGI
                     EEVANELGIKLNYDVSDVKIHSEKAHVLKYMDIIKPIVDSDYIINLCKLKTHIMATFT
                     GGTKNLYGCIAGLKKAEIHYRFPTEELFCENVLLDICDYIKPTLTIMDGIVGMEGDGP
                     SAGTTREIGVLLASQNPYAIDVVACKIISLLPNRVATIRGAIKRGYIQEDFSDIEILG
                     EDIKDLIIKNYKIPNVSKDLRLFSVKLPKFLNEPISKLITPKPVVRFQDCIKCGKCKE
                     ACPASTIEIGTKGAIIDLSKCIRCYCCHELCPKKAIDVKRNFVFKYVK"
     gene            complement(13655..14200)
                     /locus_tag="PC414_002998"
     CDS             complement(13655..14200)
                     /locus_tag="PC414_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HDF2465379.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEMVWADE
                     HRIGVAQGVDAEIIEGLRANFTGECTEVGMYLAMSRQADREGYPEVAEAYKRIAFEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKRAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRHFGK"
     gene            complement(14423..15367)
                     /locus_tag="PC414_002999"
     CDS             complement(14423..15367)
                     /locus_tag="PC414_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDF2465380.1"
                     /translation="MQKNKKEGALLKKYFGEIGLIFIAIIWGSGFVATQFALDGGLTP
                     LQIITLRFFLAAIIMNLLFFKQIRANMGKKLLKAGGILGIFLFLAFTVQTIGLMYTTP
                     SKNAFITAANVVIVPFIGFILYRRKLDKIGIISSLVALIGIGILSLEADFSINFGDFL
                     TLICSFGFAFHIFFTSEFAKDNNPMALTAIQFTVAFLMSVVVQTFAGQLKMEAELSGY
                     MGTMYLAVFSTTIGFLFQTICQKRVDGTRTAIILSTEAVFGTIFSIIILKELITAKLV
                     IGSILIFVAIITAETKLSFLKSKKVKLKDSEESSLESI"
BASE COUNT         5418 a         2419 c         1949 g         5702 t
ORIGIN      
        1 aattaatata aattaaaata aagataatat aaacacagga ggtacattct ttgaaaaaca
       61 ttatttcagc aaaagaactt atatcaaaac tagaaaaaaa tgataatcta gttgtaatag
      121 attgtagatt tgatcttatc aatagaactt atggtattga ttcttataaa aaaggacaca
      181 tcaaaggcag ttttatctta gatatagaca aggatttatc atctcctaca aaagaacatg
      241 gtggtaaaaa tcctttacaa gaccctttaa tactaaaaga aaaattagaa aaaatgggtg
      301 ttgataatga cactacgatt gtaacttatg atgatggaga tttaaatgga gcttgcagat
      361 tatttttcca attaaaacat ttggggctta aaaatgtcta tgtactagat gggggaataa
      421 catctttcgt aaaggaaggt ggagaattgg aagagaaagt gaatatacca agttgtacag
      481 gtaaggaaat tagaccaaat ataaacaatg atttagttgt tccaatggaa tatgttaaat
      541 ctaaattata caaaaaagat actgtaataa tagattgccg tgctaatgag agataccagg
      601 gtttagttga acctgcttac tcaaaagctg gtcatatacc aagtgcaaaa aattattttt
      661 gtaaggattt aataaaaagt gactttgaaa atggctcact taaagatata gaatttttaa
      721 aaaaattctt taaagattta aataattatg atgaagtaat tttatcttgt ggttcaggga
      781 tatctgcttg tgttaacagt cttgctctta gagaactgga tatacctcac aagatttata
      841 ttggtagctt tagtgactgg attagttatg atgacaatga aatcaaaact ggtcaagaat
      901 aaggctcata atctatataa aatcagcata tgatagctta aattttgtaa aggcgtcagt
      961 cattgtagac tttcgccttt acttattatt tcatatactc tttaaattta ttatatatat
     1021 tttagttgat aatattaata aactgcaata tgaccatcac atcttgattc cgtcccacaa
     1081 atataacttt cctgttctgt tttaaatatt atttgacctc gtcccatgtc gacagtatca
     1141 tgaattactt ttatttcatg acccttttca attaatgaat ctattatatg ctttggcatc
     1201 tctggttcaa cttctatttc ttttcctttt atccactgcc atcttggagc atctagcgcc
     1261 tcttgtggat ttaatccaaa atcaatcata tttgttaaca cttgaatatg tccttgtggt
     1321 tgcataaaag ctcccattac accaaatgga cctattgctt tatcttcttt acctaaaaat
     1381 ccaggtatta tagtatgata aggtttttta aatggtttta ctacattgtg gtgtttaggg
     1441 tctaaattaa aattatttcc tctactatgt aaagcaatcc cagttcttgg tatcacaatt
     1501 cctgaaccaa aatttatgta attgctctgt atatatgaaa ccatatttcc atccttatct
     1561 gcagtacaaa ggtatactgt accaccacaa tatggctctc ctgcagttgg atacaaagcc
     1621 tctttattgt caatcaataa acttcttttt ttagcatact cttggcttag taattcctga
     1681 atctttacct tcatatgctc tatatcagtt acatatgttt tagaatctgc aaaagctaat
     1741 tttaaactct ctataatttt atgtatgtct tctacatttt ctatatttcc ttctaattcc
     1801 aattctttta atatgttaag tgccattaaa actgttattc catgaccatt tggtgggatt
     1861 tcaaatattt tatatccctt atattctgta cttataggtt ctacccatgt tggataaaaa
     1921 cactctaagt cactctttct tatagcccca ttaaattttt tagaatattc atctatttta
     1981 tcagcaagtc ttcctctata aaagctttca gcctgagtat ttgctatctc ttctaatgta
     2041 cttgcctgtt cttcacatat aaatatatct ccagcctctg gagctttacc atctttagaa
     2101 aatgtatcaa accatggttt gaactcttct ccaataagtt ctttttcata gagttcatat
     2161 gattttttcc ataccttagc tacattagga gatactacgt atccctctct tgcatagttt
     2221 atcgctggag ataagcattc cattaagcta agtttaccat attttttatt taattctgac
     2281 caagctgctg gtattccagg tacagttact gctccaaacc catattttgg catttctttc
     2341 atattattat aattttttaa ttccatattt tctggagcaa agccactaga atttaaacca
     2401 tacaatcgtt tttcttcctc tatccaaaca agggcatatg cgtctcctcc aatcccattg
     2461 cttgtaggtt ctactactgc taatgtggcg gctgttgcaa ctgctgcatc aacagcattt
     2521 ccaccctttt ttagtatttc aagtccagct tgagatgcta atggtgttga tgttgcaact
     2581 gctccatttt tagcatacac gacatttctt attgatgagt atttatgttg gtaggcatta
     2641 aacttcataa atttcagctc ctcgtcattt tttatttgat attctgctta gatttattat
     2701 ataacatcaa gtaatgtaac tgatacaaaa tgatataaaa tatagacttt ttcctaactt
     2761 taattttgtc tttaaataaa tatcttaaac tcaattctag tattttctat ctgcacttaa
     2821 ataagttatt ttattcaaaa catatatcta attcttttga cttaaaaata atttattctt
     2881 ttatcatgtt tagagcatat tttaaagtta aaaaataata tataaaatat cctataaaat
     2941 aaatgactag agttatgata attggtacaa gtaatgacct tggtataaac ttttgtgcta
     3001 tagacatagc aaataaatta tgaactgtac ctacaactaa tggcattaaa aatataactt
     3061 ttaactgttt tgatattatt ttctcaatat ctttattatt tgctccaagt tttattaaca
     3121 taatatatct ttccttatca tcatatatat ttgataattg tttaaataag attatactac
     3181 ctgtacataa aagaaatact acagacaaaa atatgcctat aaacagcatt agaccactta
     3241 attttatcaa gttattataa ctttcaatac ttgatgtaaa attaaaagga taagtaaatt
     3301 tcatattttc acttactata ttttttaatt ttaaagtcaa atccagttct tttttttcat
     3361 tttttatatc aattaatctt attaatctag aatttccatg taattttaac tgattaaata
     3421 cgtcatcttt tgctacaatt aaatcaaacg tagcttgtga atttatttct ggctccaaga
     3481 tgcttttttg tactttaaac agatttatat catactcatt tgtatcttta atatgcaagt
     3541 ttattttttc aacctcaaag tctttaaaaa atattttttt gtatgaatca gaaacatagt
     3601 acacagattc ctttgaagtt aattcttcat aattgttatt ctgatattgt cttaattttt
     3661 taaaatcact ttctctaata atatctacag acgttctatc ttctatataa ctatttccat
     3721 ttttataata catttcaata tttatatctt tgtctataag ttctattgtt ttatcaaaga
     3781 ttatcttatt atctttttta tatttatata ataatttgtc aatttcgtta tttacataag
     3841 aatttcctaa atttatggta taactgtatt tataattttc acttatgttc ctatctatat
     3901 cgtaataaag tgatatggta aaccctagca tagttacact tgtagctatt aatattgcta
     3961 ttacagccaa tacactacta ttgctcttta atttaaatcc aagttcagaa aatgcaatta
     4021 aattctgtcc cttataataa aaactttttc tcttcttaac agcatcaata atcatataca
     4081 tcgttgatga aaaaaataaa aaagttccag gtattattgt aactaatata aaaactgaaa
     4141 gcataatatt ttcagcaata aaataactaa ttgacattaa ataagaaata gctattaata
     4201 aaacaccaag tacacatttt aactttataa attttttaaa cttatacttt tctggatatt
     4261 tattaaataa ctctataatt ctcttatttc tgataactat agtatttctt attccaataa
     4321 ctatgaatat tattaaaaat accgctagtg tctgaactaa ggcctttata cttaagttca
     4381 tatttactac tatcatttca ttcattaatt ttacaagcat catagtcata agctttgaaa
     4441 ataacagtcc taaaaatata cctattagta ttgcaactat acccatcaaa aaagtttcat
     4501 ataaaaatat ttttcctatc tgatttttcc tcattccaag taaactataa gttcctactt
     4561 ctcgcctacg tctattgaaa aaaaatgagt ttgaataata tataaataaa aatacaaata
     4621 ccactattac aattgaactt gtattaatac tcatcctagt tttctctcca agtgaactca
     4681 acgcttcatt atactctata gatttaaaac taaaaaatac aaatacacta aatacaattg
     4741 aaataaagta cattaaataa ttaaaaaaac tattctttac attttgcatt gcaattctaa
     4801 ataaagtcat ttttattaac tcctccaatt aaggaaactg tatttactat tttattatag
     4861 aactctctgt tactttctcc ctttacaatt tctgtgaata ttattccatc ctttataaag
     4921 agtattctat tgcaaaaact cgcacttata gcatcatgag ttaccatcaa tattgtttta
     4981 ttgctgtctt tgtttaattt taatagacaa ttcaacaaat ccctagcaga tttagaatct
     5041 aatgctcctg taggctcatc tgcaagtact aatgatggtt ttgttgctat agctctacat
     5101 acagctcctc tttgcttttg tccaccagaa acttcgccag ggtatttatc taatatagat
     5161 tctatattta attcttttga aatctctttt attctatttt ctattactga tactctttca
     5221 ttttttaaag aaagaggcaa cattatattt tcttttattg taagtgtatc caaaagatta
     5281 gaatcttgaa atacaaagcc tagattatca cgtctaaaat ctgccaattc attttgcttc
     5341 attttaatag tattctttcc atttatataa atatctcctg atgaaggtaa atctattgta
     5401 gaaataatat ttaaaagtgt tgatttacca gcaccggaag accccataat accaacaaat
     5461 tctcctttat aaaccttgaa agttattcct cttagagctt catgttttga accttttctt
     5521 ccatactcct tttttatatt ttcaacagat aatatttctt ccatagttaa taatcacctt
     5581 tcatcacaat tagttattta tcaattctca caaatatatt atatatatta aaatggcttt
     5641 taaacattta attatattaa tttaaggtta cattttcgta agtattaatt atatttcttc
     5701 aaatttattg caattatgtt taaaatataa tttatcaagt aaaatatagt tatattaata
     5761 taattatttt aggtaataag gtagaaatat tttaatttat caagtagaat atagttatgt
     5821 taatataact attttgaatt aggaggtaag aatgtttaaa ataatggtag ttgaagatga
     5881 cgtctcattg aaaaatatca tagcaaagtg tcttactaaa tggggacatg atgttcatca
     5941 aatagaaaat cttgaaaata taatagaaga gttcaaaaac tataacccag aattagtttt
     6001 attggacata aaccttccat tttatgatgg ttttcattgg tgtaatgaaa taagaaaaat
     6061 ttcaaaagtt cctataattt ttatttcttc aagaaattct aatatggatg taataatggg
     6121 tgtaaattta ggtgcagatg attatataca aaaacctttt tctgtagatg tcttagtagc
     6181 taaagttaat gcccttttaa gaagaactta taattttgtt gataataact caaatcagat
     6241 tattcacaat ggtgttactt tagatttatc aactgcaaca ataaactatg aagataatac
     6301 tattgagcta acaaaaaatg aaatcaaaat tcttcatgag cttatgaaat ataaggggca
     6361 gatagtaagt aggaataaac tcatgaaaaa attatgggat aatgattggt ttgtagatga
     6421 taatacatta actgtcaatg taaatagaat acgctcaaag ttaaatgaaa ttggtttaga
     6481 agattttata gaaacaaaaa gaggattggg ttatataatc agttaaaaca attctaagta
     6541 ggtgaattta tgagctttaa agattttctc aaggataaac taggtttttt agtatacaat
     6601 tttactttct taatttttac agtatcagtt attatatttt ctcctgtaga tgtttttctg
     6661 actgatacta ttttatatat attagttgta aatgttgtgt ttttattttt atatctattt
     6721 attagctaca tcaagaagaa caaattttta gataagataa aaaatgatac attcaaaatt
     6781 aatatgaatg atatagaatt agctagatgt aaaaatgaag aaaaaatata cttgaacatt
     6841 attaaaaatc atcaatatca atgtaataac ataataaata ataatatcga gaaatttgaa
     6901 gaaaacaaag agattttaaa tatgtgggta catgatgtaa agatacctat agctataata
     6961 aaactcattc ttgaacaaaa tgaaaattta attgatgaaa aaatttcaga tgacatagat
     7021 agtgaaatat ttagaattga aaactccgtt gaaaaaattt tatatttatc aagactcgaa
     7081 gattttcata aggatttttt aattcaagag gtaaatattg aaaaaatcat tagaaatatt
     7141 ataaaaaaat actcaaaata ttttatttca aaaaaaatta ttttagactt aaataatttg
     7201 aattttaagg ttttatctga tgaaaaatgg ctatacttca tattcgagca attattgagt
     7261 aattccctaa aatactcctc attaggagat aatatatcta tctattgcaa aagaaccaaa
     7321 aattatttac aattaagagt ggaagatact ggttgtggta taaaaaagga agatttaaat
     7381 cgaatattca ataagggatt tacaggtaat aatggaagaa acaattctaa atctacaggt
     7441 cttgggcttt atttagtaaa agaactttgt gataagcttg gtcataaaat tgatgtagat
     7501 tcagagtaca accaatatac aaaatttact attacattca aatgtgattt atagtatctt
     7561 attattgata gtttattttc attaacttat attcaacttc ttatagtaat tctatatgta
     7621 catcccagta tataattaag tagtttttat aaaaaggagg aagacatgtc ttttctaaaa
     7681 aaatttaaaa ataaaagatt tagaaataac agttttaaaa gtaacaactt tagaaatagc
     7741 agttttggaa gtagtggctt caagtctaaa aataaaggtt ttaaaaacaa tccctttaaa
     7801 aataagagat tcaaaatata tgcaggaata gcagtaatca tacttttaat tctaggagtt
     7861 cttgcttatg ttgattcgaa aaacactgaa ttacaagcta atgaaaattt tatcgaaaca
     7921 tatactattc ctgaaaatga aaaaatattt ataaatggga tggttgttcc taagcaaact
     7981 aaggacttca atatatctgg agattatgag ttaagtgatg ttaatgttac aaatggtcaa
     8041 aaagtaaatc aaggagacct tctttttact gctaaaaatc caactataat agcagaaata
     8101 gatagtttaa aatcacaact tagtcaatac aaaaaacaaa aaatatcact ttctgatatt
     8161 gctgaaaata aagatgctat tgcttctata aatgcacaga taactgcttt aagctcccag
     8221 atagcttctc tggataaaaa agcttatgac agagttactg ctccttttga tggcacagta
     8281 tacttgaatg accaaactgg aaatccagac caaccagttt catttatgac tattcaaggt
     8341 ctagagtttt atatgaaagg acaagcaagt gaacaagatt tacctaaaat gaagattgac
     8401 caaatggtca atatactagt tttttcaact gaccaaaagc ttactggtag aataagtttt
     8461 atatctgata aaccatctac tccaaatact gaaatgggag ctcagcagaa tacactatct
     8521 tattatgata taaacatagc atttgataat caagaaggat tagtaaatgg attccatctt
     8581 caagcatcac ttgaagtagc taattcaagc tttaaaatac cagcatcttg tgtacttaaa
     8641 gacaacaaac actcatatgt atttaaggat ttagatggta tattgaaaaa gcaaattgta
     8701 gatgtagcaa gtcaaaatga tgattttgct gtagttagag gcggtcttga acaaggcgat
     8761 ataataataa aacaccctac taaagaaatg aaagaaggag acccagttca aggcgatggc
     8821 gtaactgctg gttctaataa tggagatact actccaaaca aaaaagtaga agaagttact
     8881 atggatgtga attaatttac atatgttaat taaattagaa aatattcaaa aatattacaa
     8941 agtagggaaa gatgaattac atgttttaaa atccttaaat ttagaaattg aatctggaga
     9001 atttgtcatg ataatgggta agtctggtag tggtaaaact actttactta atatcttagg
     9061 ttttctagat gtatttgatg aaggaagata tatatttgat ggaacagatg taactaattt
     9121 gagtgaaaat gaacgttctg tttttagaaa tattaatatt ggatttgttt tccaacaatt
     9181 taatttaata gaaactctaa atgtatatca aaatgtagag ttgcctctaa tttacaataa
     9241 agcactaaaa aaatctaata gagaagaaat tgttaaagat aaattaagtt ctgttgggct
     9301 tttggacaag cttaagcaga aaccacttca attatctggt ggtcaacaac agcgtgtagc
     9361 cattgcacgt tgcctagcaa atgaccctca gataatcttt gctgacgaac cgactggggc
     9421 attagatagt gaaactagca gagaaattat ggagctttta acaaggctca acaaacaagg
     9481 taagactata ataatggtta cacatgatca agatttaact aagtatgcaa ctaaggttat
     9541 acgacttaaa gatggtgtgt ttacttcgga ggtgtaatca tgagtttatt aaaaaattct
     9601 ttggccaatt taaaaggtca caagcttcgt gtatttgtag ctttactctg gattataata
     9661 ggaataactt cagtaatact tgttagttct ataggtaatg gatttcaaaa agaaattaag
     9721 aaatctgtaa acaatgtaaa ccctaataaa actactattt cgtttgaatc agccgataac
     9781 acaggtctta ctgatgatat gagtattttt ttaaaaccat ttaatgcaaa agatttagaa
     9841 gaattatcat ttgttgaagg agtagaaaga atagctcctt caagagatgg gtttaacctt
     9901 gattcagttt actcttcaca agcatctttt gataaaaaaa ctacgtatgt tgatgttgga
     9961 ccagtaaaaa aagattctaa aataaattta atttgtggta gagatttttc tttagatgat
    10021 gaaaaaagaa aagtaatttt acttacatta caaagtacaa gtgaaatttt tgaaaatcca
    10081 gaagatgcct taggtcatgg tatcaacata aatggaacta tatttgaaat aataggggta
    10141 ttagatgatt ctcagcaaaa ccaagctgga ggattctttg gaggttacca agatatgcaa
    10201 tttactactt ccctagtacc taaaaaggct tttgacactt taatgagtca aaattcttat
    10261 tcaaatgaaa tttaccagtt agacttagtt tcttctaaag gctataatgt aaatgaagtt
    10321 gcaaacaatg ttatagctaa attatatgag atgcaccctg gtatcaatgg ttcttatact
    10381 acaccagacc caactgaaca gacagcttat cttgaaagca ttaattccaa tgtaaacaaa
    10441 tatgtatcta taataactgt agttgcaatg tttgttggtg gtattggtgt aatgaatata
    10501 atgtatgtat ctgttatgga aagacaaaga gaaattggta taagacgtgc tattggagca
    10561 aaacctaggt ctatattatt tcaattttta gttgaagctg tttttataac agtatgtggt
    10621 ggaattttag gaactatagt tggatttact gctactaact atgtttctaa atatataggt
    10681 tttgaagcta tacctagctt aaatagctta ttttatgcaa ttgtagctac tatattgact
    10741 ggagttgtat ttggtctgat tccagccttt aaagcctcta aactagaccc tataaaggca
    10801 atttataagt agaaattgta taaagatttt cgatttttag attagtttat ataaaacata
    10861 cttaaatata taacgcacta ttatatatga tagattagga acttaatctt tccaaatata
    10921 atagtgcgtt ctttcataaa aaggattctc ttccttacta tatctaaatt accaagatgt
    10981 tatgttctaa ttgttcaaac taatacttcc aattttgatt aatcaatctc attctcctaa
    11041 tatacaatct tttactttca atcattgtac actccgccta aaaaaaataa tcttaaatta
    11101 ggcgttatat acaatctccc cattacatat tgtataatta acctttccat ataaagattt
    11161 acctttaaat ggtgaattat cagctttaga aacaaagctt tcaactgtcc attcttcttc
    11221 tggattgaat ataacaatgt ctgctacagc cccttcttct ataaatccaa tatttaaatt
    11281 gtacaacttt gcaggattta tagacatttt ttctattaac tgcatcatac taagatgatt
    11341 tttatgcacc aaatttgtta ttccaagagc taaagaagtt tcaagcccaa taattccact
    11401 tggagctttt ataaactctc tatctttttc ttctttacta tgaggtgcat ggtccgttgc
    11461 tattatttca atagtaccat catttaatcc ctctataatc ttatctctgt cccatttact
    11521 tcttagaggt ggattcattt ttgcatttgt tccaaactcc aatacatctt cctcagtcaa
    11581 tgtaaagtga tgtggtgaag cttctgcaac tacatttgca cctaagtatt ttgcaaatct
    11641 aaccatatca acagatacac ctgaacttat atgttgtata tcaactttag caccagtctt
    11701 tagtgccaac atacaatctc tggctaccat aacatcttct gctacacttg atgcaccttt
    11761 taatccaagt tctgaagcta cctttcctgc atttacacct ggatttccaa ctaaagaagg
    11821 gtcttcttca tgaaaactaa gtggtacatc caactctttt gccttaatca ttgcctccat
    11881 aattagattg ctatccatta aaggtaatcc atcatcagta aaccctggta caccagcttt
    11941 tttaagagct tccatatcaa ctaactcttt accttcaaaa cctttagtta tagctgctgc
    12001 ttgtaaaaca tttatacatg cattttttga cttttcttta acataattaa aagtatcttc
    12061 attatctaca ataggattag tatttgccat acatataaca gttgtaaatc cacctctagc
    12121 agcagattta gctccacttt ctatatcttc tttatatgta aacccagggt ctctaaagtg
    12181 tacatgtaca tccactaaac ctggagcaac tacattacca ctagcatcta ttattttttc
    12241 atattcatca ctttcatcaa acttgcctat tttataaatt ttattttctt ttataattaa
    12301 gtcaatattc tcatctcttt gacttttagg gtctattact ctaccatttt taattaaaat
    12361 catattattt ctcctttata tttttaattt atatagactc atataatatt atacattatt
    12421 ttacatactt aaatacaaaa ttacgtttga catcaatagc ctttttagga caaagttcat
    12481 ggcaacagta acatcttata catttactta agtctataat agcaccctta gtccctatct
    12541 ctatagtgga tgcaggacaa gcttccttgc attttccaca ttttatgcaa tcttgaaatc
    12601 ttaccacagg ctttggtgtt ataagctttg aaataggttc atttaaaaat tttggtagct
    12661 ttacagaaaa caatcttaaa tccttactta catttggaat cttgtaattt tttataatta
    12721 aatcttttat atcttcacct aaaatttcaa tatctgaaaa atcttcttgt atatatcctc
    12781 tcttaatagc acctctaatt gtagcaactc tatttggaag taaacttata atcttacatg
    12841 caacaacatc tatagcatat ggattttgtg atgctaataa aacgcctatt tctcgagtag
    12901 taccagctga aggtccatca ccttccatac ctactatacc atccataatt gtcaatgttg
    12961 gttttatata atcacaaata tctaaaagta cattttcgca aaacaactct tctgtaggaa
    13021 atctataatg tatctctgct tttttcaatc cagctatgca tccatataag ttttttgtac
    13081 caccagtaaa agttgccatt atatgagtct ttaatttaca aagatttatt atataatctg
    13141 aatctactat tggtttaata atatccatat attttagaac atgtgctttt tcactatgta
    13201 tcttaacatc tgaaacatca taatttaact taatgcccag ttcatttgct acttcttcaa
    13261 ttccagatgc cttataaatg ctcttaagag aactttgatt atatggacca ccagggctat
    13321 ctgctattgt aatatcacaa tttaactcta aaagtttttc acatacaact tttacaacca
    13381 taggatgtgt agttgtagct tcttcaggct tttttttcat taataaattt ggttttataa
    13441 gtactttgct tcctttttta acaaacttat ctataccacc aatatcctct aaattttttt
    13501 caatagataa ctttacttct tcataattgt aagttttaca actcctaaca gataccgact
    13561 tcatattatc tccatccttc tatcacaaca gtttatttgt ataatttaaa aaaggagaaa
    13621 ccaaagtttc tccttttaat ctatgtttta attattattt tccaaaatgt ctgtttaata
    13681 atcctaagaa tgctttacca tgtctagctt cgtctttaca catttcatgt actgtatcat
    13741 gtatagcatc taatcctaat tctttagctc tcttagctaa ttttaatttt ccatcagttg
    13801 caccatactc agcatcaact ctaactctta agttttcttt tgtatctgca actacaactt
    13861 ctccaagaag ttctgcaaat ttagcagcat gttcagcctc ttcaaaagct attctcttat
    13921 acgcttcagc tacttctgga taaccttctc tatcagcttg tctactcatt gctaaataca
    13981 ttcctacttc tgtacactca ccagtaaagt tggctcttaa tccttcgatt atttctgcat
    14041 ctacaccttg agctactcct attctatgtt catcagccca aaccatttcg cctttcattt
    14101 cttcaaattt atcagctcca actttacata ctggacattg tgctggtgca gcatctcctt
    14161 catgtatata tccacatact gtacaaacaa attttttcat aattaatttc ctcccaattt
    14221 tatataatta aatttttatt tttaagttta ataacttgct acaagaatat atatacccca
    14281 gactttatag cttaaacact aaaaataaaa tttatttcct aattaatttt atcactttga
    14341 aacaccagta tcaaccaagt tttgatgtca aatttctaca taatacataa tttatttgat
    14401 taattaggaa atagttgtca tattaaatac tttctaatga agactcctct gaatctttaa
    14461 gtttcacttt tttgctcttt aaaaaagata atttagtttc tgcagttatg attgcaacaa
    14521 atatcaagat actaccaata accaattttg ctgtaataag ctcctttaaa attattatag
    14581 aaaatattgt accaaataca gcctctgtgg ataatattat agctgttcta gttccatcaa
    14641 ctcttttttg acatatagtc tgaaataaaa atccaattgt agtactaaat acagctagat
    14701 acatagttcc catgtaacca gataattcag cttccatctt taattgacct gcaaatgttt
    14761 gaactacaac agacattaaa aacgctactg taaattgaat agctgtcaat gccattgggt
    14821 tgttatcttt tgcaaactca cttgtaaaga atatatgaaa tgcaaatccg aatgaacata
    14881 taagtgttag aaaatctcca aaattaatag agaaatcagc ttctaaagaa agtatcccaa
    14941 ttcctataag agctaccaaa ctacttatta ttcctatttt atcaagtttt cttctataaa
    15001 gtataaatcc tatgaatgga actattacaa cattagctgc tgttataaac gcatttttag
    15061 atggtgtagt gtacataagt ccaatagttt gtactgtgaa tgctagaaat aaaaatattc
    15121 ctaaaatccc tcctgctttt aataattttt tacccatatt agcacgaatc tgtttaaaaa
    15181 acagtaaatt cataattata gctgccaaga aaaatcttaa agttatgatt tgtagaggtg
    15241 taagcccacc gtctaatgca aattgggttg ctacaaagcc acttccccat ataattgcta
    15301 taaatattag tcctatttct ccaaagtatt ttttcaataa agccccctcc tttttatttt
    15361 tttgcaaaat tataaacccc ctaaacagca agagagtttt tctataagca ttactataaa
    15421 ttaatatagt aaattttttc agtattttaa atctaaattt acatacattt tgagctcaaa
    15481 tttatatc
//
LOCUS       DAMJVK010000095        15006 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.099, whole genome shotgun sequence.
ACCESSION   DAMJVK010000095 DAMJVK010000000
VERSION     DAMJVK010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15006
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.099"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(222..605)
                     /locus_tag="PC414_003000"
                     /pseudo
     CDS             complement(222..605)
                     /locus_tag="PC414_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     regulatory      831..928
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            983..1159
                     /locus_tag="PC414_003001"
     CDS             983..1159
                     /locus_tag="PC414_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465381.1"
                     /translation="MFGNKMDKCTHVLTAYISSSYDYCNFLDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     gene            1385..1585
                     /locus_tag="PC414_003002"
     CDS             1385..1585
                     /locus_tag="PC414_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDF2465382.1"
                     /translation="MNNGIVKWFNNEKGFGFISMEGRDDVFAHFSAIQTSGFKSLEEG
                     QQVSFDIVKGARGPQAENITIL"
     gene            2337..2519
                     /locus_tag="PC414_003003"
     CDS             2337..2519
                     /locus_tag="PC414_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HDF2465383.1"
                     /translation="MFKFYNNATLMFLSGTNIKTISERLGHADIKITMNSYSQFLEEM
                     DREAFENLSKILFIIF"
     gene            2590..3435
                     /locus_tag="PC414_003004"
     CDS             2590..3435
                     /locus_tag="PC414_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDF2465384.1"
                     /translation="MNIIKSITLLLTLLIVILMTGCSPRESASEVAKKYIDKMNSGDY
                     ETAYSLLSKDSKKKISIENLEEFQNILVSTKRIISYKIGKEKIIKKYKHDGKEYKNVV
                     KLEETFNIKNLLYKKDDSLKVNRYFVVENNKYKLLLYESFKKEFGINSMDLAQLKLND
                     FGLKYFKEVDLILKKAIAINPTDSKLYYAQACNYMNFDGFPGYSDTTYNALDSINKSL
                     KYLDKNDSDYKKNASNIYNLKGVILMFHKRFDEAQQYLNKALYFNKDNEDPKYNLNTI
                     KKHLN"
     gene            complement(3659..3823)
                     /locus_tag="PC414_003005"
     CDS             complement(3659..3823)
                     /locus_tag="PC414_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465385.1"
                     /translation="MFGKPKESVRFLDFNDVAEKFGEKKAVAIFDAIAGKKSKEHIIK
                     DIEKLVKNEV"
     gene            complement(4365..4697)
                     /locus_tag="PC414_003006"
     CDS             complement(4365..4697)
                     /locus_tag="PC414_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDF2465386.1"
                     /translation="MNTREMKEYRGFYNKKLSETDEFFRVFGSLDDKTYSEGAISKKN
                     KELMGLAISILSRCDECICYHIEGCINSGASKDEIIEAIKIGVIGGGSITYPNARFAI
                     KILEEFNI"
     gene            complement(4941..5582)
                     /locus_tag="PC414_003007"
     CDS             complement(4941..5582)
                     /locus_tag="PC414_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HDF2465387.1"
                     /translation="MKKAINKQFILSTLICFIPFIVSIYFYNRLPNEVAIHFDNYGNP
                     DNYAPKVIAAFGVPLLMLCIHLYTWFRLENEPKKANFSNVIQNLSKWGVAILSVIIQI
                     MMVLYSTGVSLESNFYTGLILGIIVILFGNYLPKCKRNYSVGIILPWTLNDEDNWNKT
                     HHLAGWIWLIGGILLIINAFINIPFYNIFVIFVIVILPFIYSYLMYKISGKNN"
     gene            complement(5563..5856)
                     /locus_tag="PC414_003008"
     CDS             complement(5563..5856)
                     /locus_tag="PC414_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF2465388.1"
                     /translation="MGFPETFKALSDPIRREILVMLRENKMSAGEISEKFNMTNATIS
                     YHLSQLKKAGLIFETKYKNFIFYEINISVFEEVMLWFSQFMEKGEKDNEKSDK"
     gene            6249..6308
                     /locus_tag="PC414_003009"
     CDS             6249..6308
                     /locus_tag="PC414_003009"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDF2465389.1"
                     /translation="MTEYIYYYNNKRIKKINLA"
     gene            complement(6824..7576)
                     /locus_tag="PC414_003010"
     CDS             complement(6824..7576)
                     /locus_tag="PC414_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HDF2465390.1"
                     /translation="MFSVIFLCIGGFLAAFVDSIAGGGGLISMPVLMAIGVPVHLAIG
                     TNKFAASAGCISSAYRYAKSGKINNNLLKKLVPFTIIGSVLGVRCVLSISEEILNVLV
                     VVMILIVAIYTFISKNLGQEDNFEAVNKKNLRLGMLMALIMGFYDGFFGPGTGTFLTF
                     GFIKIYGYDFLHASANTKILNLTSNITSLLLFMINGQVDYKIAIVFALVMIMGAYVGA
                     KVAIKKGSKMIKPIFLVMALFMVVKLVYQTLV"
     gene            complement(7635..9290)
                     /locus_tag="PC414_003011"
     CDS             complement(7635..9290)
                     /locus_tag="PC414_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2465391.1"
                     /translation="MEYFSESNNEVQKFDWGEVMWIHEPSKTQFNRLSAGIVRFFPGN
                     YQEKHFHLSEEQLLYVIQGEGIQIIDGKKVNIKETSIVYCPPYSEHEIINTGKIDLVI
                     LITYVPHKFSSLRQIPIVFSENNIQELVNVNIIENLANQISNILKLRISVYDLKYKEI
                     FETKKENKFCDICKNIKQCTKKLVKSINNDNFEKVYHCEYGVSSLEIPIVLEENIVGY
                     IECGNFIVYKSIDIDKNLSELSSSSGIDIKYINKIYNEFPLNPKSRLYVLKENLLMMS
                     EFIQEIAKRNFFENQLSIKDEEILKSRKENIRLEEALKKANSRLLEEEYIINPIDMFN
                     NSSKEYPFELELYIEKEIKNMNLEGVYNLIDTNKLRYNDVRDTIQEMIFVLSRTVLRD
                     LEDLKLISYLRNKYNKKISLVNSDEDLWNVLFEFSKECIDKNRAFWRKDKGNLIENIN
                     EYIKKYYKENINLNSISDVFFISPNYLSSIFNERNKVSITEYINLLRIEESKKYLLDR
                     SMSISDICKKVGFNNSSYFSQIFKKFNSITPNEYRKNMLDNKY"
     gene            complement(10563..11642)
                     /locus_tag="PC414_003012"
     CDS             complement(10563..11642)
                     /locus_tag="PC414_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2465392.1"
                     /translation="MILITLCILVAVFVLAFAGLRLIAPTIIRTSHKIEAPGIDRMEM
                     VEIGGIQQALYIRGQNADNPIILWVHGGPGNSMMPFLHLYQYEWENDFTVVNWDQRNV
                     GKTYFANDPAAVLQTMSAERVLQDVHEVTAYLKQEFNKEKIILIGHSWGTVLGTMAVQ
                     TYPEDYSAYIGIAQTVNMVDNERVGYEKTLEMVKAAGKQKDIKALEAIAPYPPAKYGP
                     SYLAALMTVRTYQSKYGLSINMDLGNIISILATPYYSLRELKNTAIFNPNAIDNQGDV
                     LRFLVEEADIRNYGVDYQVPVYYIMGENDYNSPYLPAKKFFDEIVAPDKQIFFIPDAD
                     HLPFLSKKAEFDCVLLEEIAPKLAK"
     gene            complement(11726..12382)
                     /locus_tag="PC414_003013"
     CDS             complement(11726..12382)
                     /locus_tag="PC414_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2465393.1"
                     /translation="MTRKNNPKQAIENIITISAKLFAEKGYDKTSMQNIVDASGMSKG
                     GIFHHFSSKEDIFNVVMERRFEQIIETVNQWLGEMHGLTAKEKLRSLVRRHLTDEVIK
                     ESSNMITSAIESPHIILAFTQDNLKKLAPILANVLREGIEDRSIITEFPDECAEVILL
                     LFNFWCDTDVFQGDFPTLRKRFQFLQFLMRQIGVDILEDEIIESINIFYERKPMGQVE
                     "
     gene            12704..12910
                     /locus_tag="PC414_003014"
     CDS             12704..12910
                     /locus_tag="PC414_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465394.1"
                     /translation="MISHAAEICKKYKMPFIIFPTIEKKSIEEWVNIGANMLCLAQDT
                     TFITYVINQLLSFYNEIVPIQKQH"
     gene            complement(13063..13491)
                     /locus_tag="PC414_003015"
     CDS             complement(13063..13491)
                     /locus_tag="PC414_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDF2465395.1"
                     /translation="MSKIYIVYWSGTGNTEKMANFVAEGVKLKGKTPEVLDVSLLKPS
                     DLKEEDKFALGCPSMGAEQLEEGYMEPFVSELESMVSGKQIGLFGSYGWGNCEWMRDW
                     EERMQNAGATIIGGEGITAMEDPNEEAKDECIELGKTLAE"
     gene            complement(13967..14905)
                     /locus_tag="PC414_003016"
     CDS             complement(13967..14905)
                     /locus_tag="PC414_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HDF2465396.1"
                     /translation="MNKRMKLIPYEINENLRGAKNKFPYGIKQMNARGMWDEGYTGKN
                     IVVGIIDTGCDISHPLLKGKIIGGANFSDDSNGNKNIYEDFNGHGTHVAGIIAASNYN
                     NEVMGVAPDCKLLIAKALNKDGTGTYQSIINAINFAVNNKVDIISMSLGGNKDDKNLK
                     NAVMQAVKNNISVVCAAGNNGDGDSSTSEYSYPASYAEVIEVGAINENYLVEKFSNSN
                     TTIDLVAPGRNIISTYMDNKLAIMSGTSMSAPYVSGSLALIKEWAREEFERDLDEAEL
                     YAQLIKCTRALGIPRTEQGNGYLYLNLYKYKNNSKR"
BASE COUNT         5214 a         2432 c         1607 g         5753 t
ORIGIN      
        1 aatataaaag acagtaattt atctatagtg ttacttatat tttatatata tggctatttt
       61 tcaacatttt acaatatata acatttattt ttttaacttt tgtcagtaaa atgtcagcaa
      121 aacactttat ccacaatttt tatataagtt ttccacaact tatcaccaat attatccaca
      181 taaaaaaggt agcacttata ttattagcta ccttttattt attaatcttt atatttccag
      241 ctttttatcc aacttcctat ttctattatt ttttcttcag ttatttcttc tccatcatgt
      301 aattttgaaa ttaattctga taaaggattg ttatgtaaac ctctaaaaac tctttttccc
      361 tctattttga gatattctct ctcttttatt aatactgtat agtgtacatg cctcccaatt
      421 ctctatgata ttaaaaaacc ttttttggtt aatctagcta aaagtataat tgtagttgta
      481 tgcttccaac catatgtttt ttctatttca tctgcaacat cttttgatat tacagtttta
      541 taaccagtat gccatatgca tttcataatt tttaattctg tatctggtaa ttttttattt
      601 agcataatat attccttctt tcaatttatg tcacttcttc aaatttatct agcttaaatt
      661 ataataggag ctttttagaa caaaaactat atttaatttt taccaggtac tctacgtgtt
      721 tagtctgttt ttaagtgtaa aataatgttc atatctataa atttatttaa ctttcaaaca
      781 atcctgttga ataataacta attctattgt attattttag ttgtagaata aaactaaatc
      841 ggcaaaacta gagaaattta gtgacgcaaa gctataggga ctaagactta tataaatatc
      901 ttatgagtta tgtcagccag ttgccaaaga gatagtgttc tttttgtttt tatgaaagtt
      961 ttttaggggg ataataattt atatgtttgg aaataaaatg gataaatgta cacatgtatt
     1021 gactgcttat attagcagtt cgtatgatta ttgtaatttt ttagatacac agttagatga
     1081 ttttatatta gagtacggag aaaatgtagt agagtcttgt ttacaccaag taatggtatt
     1141 ggtaagtaag tataattaga tagacattga tttctagaaa acccttttta gttttatcta
     1201 catttatatt aatatatagc aatgtaaaac gtttaaatat atttttaata ttaagaataa
     1261 ttaaaatgtt aactaaaaaa tataattaag tagacatttc tattaatcta tgatacaata
     1321 gtatttgtgg taatacacaa ttaaatcaag tatatcttga tataggttaa taggagtatt
     1381 tttaatgaat aatggaatag tgaaatggtt taataatgaa aaaggattcg gttttatatc
     1441 aatggaaggt agagatgatg tgtttgctca tttctcagct atacaaactt caggatttaa
     1501 atcattagaa gaaggtcaac aagtaagctt tgatatagtt aaaggtgcta gaggtcctca
     1561 agcagaaaat ataactatat tataatatat agatttttcg ggaaagactc tttttagagt
     1621 ctttttttat tgttaatata tgtaagataa tcattggtga agtaaataag gtatattgag
     1681 taatgttctt gatagtttat atgctaatga atttcaaaaa aataagcact cctataaaaa
     1741 gagtgctctt aatgtaaata taaaccttca ttggtccagc ctaatacccc gtattctcca
     1801 agttctaact attttttata tacattatca ctttcatcca tatctaacac aattatttct
     1861 tctttagaat caatttgtaa aatatgagga ctgtgtgttg tgacaattat ttgtgttttt
     1921 aggagaattg cttttaatgt aataaaaatt ttacatacca catagttgat ataaaactat
     1981 aataattaca taagattgta ataataagac ttatctttac ataccacata gttgatataa
     2041 aacaatgaaa ttaacataca agagactaag acaattaaaa tctttacata ccacatagtt
     2101 gatataaaac cccaaaataa acttagcatc tccaatgttt acacatatac aatgctctta
     2161 aaattgcagt gaacgcgcaa tagtgcaatt gataatactt attatatatt ctcaatgcct
     2221 tatatttcaa ctgctaaacc tcaatttgtc acaaatatcg ctcactgcaa aatttctata
     2281 tttttattat atcataacat ataattaata ttttcaatta atttttacat ttaattatgt
     2341 ttaaattcta taataatgca actttaatgt ttctttctgg aactaatata aaaactattt
     2401 cagaaagatt aggtcatgca gatataaaaa taactatgaa tagctactct cagtttttag
     2461 aagagatgga cagagaagca tttgaaaatt tgagcaaaat actatttatt atattttaaa
     2521 gaagatgact tattcaatat ttgatgctag aatataatta ataaaaattt aattataggt
     2581 gattgtatta tgaatattat taaaagcata actttactct taacattatt aattgtgata
     2641 ttaatgacag gctgttcccc aagagaaagt gctagtgaag ttgcaaaaaa atatattgat
     2701 aagatgaact ctggtgacta tgaaacagca tatagtttat taagtaaaga tagtaaaaaa
     2761 aaaattagta tagaaaatct tgaagaattt caaaatattt tagttagtac aaaaagaata
     2821 attagttata aaataggtaa agaaaaaatc ataaaaaaat ataaacatga tggaaaagaa
     2881 tataaaaatg ttgttaaatt agaagaaact tttaatataa aaaaccttct ttataaaaaa
     2941 gatgattctc taaaagtaaa tagatatttt gtagttgaaa ataacaaata caagctatta
     3001 ttgtatgaaa gtttcaaaaa agaatttggt attaatagta tggaccttgc tcaacttaag
     3061 cttaatgatt ttggactaaa atactttaaa gaagttgacc ttatactaaa aaaagctatt
     3121 gctattaatc ctactgattc aaagctttat tatgcccaag catgtaatta tatgaatttt
     3181 gatggatttc ctggttattc agatacaaca tataatgctt tagattcaat taataaatct
     3241 ttaaagtatt tagataaaaa tgattctgac tacaaaaaaa atgcaagtaa tatatataac
     3301 ttaaaaggag ttattcttat gtttcacaaa agatttgatg aagcacaaca atatttaaat
     3361 aaagccttat actttaacaa agataatgaa gacccaaaat ataatctaaa tacaataaaa
     3421 aaacatctaa actagatatt taatttttga atttataaaa tattttttta tgtataattc
     3481 agcactgaat cataatttct actaaactaa ttacaagttt aaattataaa gacttatttc
     3541 tatataaata ttactctata acaataagtt tcttatttaa attttaaaat attatctaaa
     3601 aatcataaaa aagaaggtat aaagagctac cctcaagctc aatatacctt ataaatatct
     3661 aaacttcatt ttttactaat ttttctatat ctttgataat atgttcttta gatttttttc
     3721 cagctatggc atcaaaaatt gcaacagcct ttttctctcc aaacttttct gctacatcat
     3781 taaagtctaa aaatctaaca ctttcttttg gtttaccaaa catataacac ctctcataca
     3841 tttttatata tttattataa cttctataga cttatttttc accaataaac gttagacaaa
     3901 ttagtccctt tattttttta aactagcttt accttcaaaa ttcttaacaa aaacagttta
     3961 ttctactcta taaataaaaa tagacaattt aatttgtagg aaactgataa tagaatacag
     4021 taaataaaac tttgtttaat ctatctgaaa aagttatcta ttaaaactgc tatgaattta
     4081 taaagaatat tctagttcat aaaaatacaa atatgtaaat ataatcacct taatattcaa
     4141 tttatttaaa ataaaaaatg tataaaagat ttaataatga caatatacta ttctcaatac
     4201 aatctatgat gtaaataatt taaaactact atattattta aaaatttata tttatatatg
     4261 caattctaaa aacttaataa gaagtgaact tagaaaataa agcttaattt caaagactta
     4321 aatagactat tattattagt ctaaataaac tacgtctatc aaaattatat attaaattct
     4381 tctaaaattt ttattgcaaa tcttgcgttt ggatatgtaa tagacccgcc accaattaca
     4441 cctattttta tagcttctat aatttcatct ttacttgctc cagaattaat acaaccctca
     4501 atatgataac aaatacactc atcacatcgg cttaaaattg aaatagctag tcccattaat
     4561 tccttatttt ttttgcttat agccccttct gaataagtct tatcatcaag acttccaaat
     4621 actctaaaaa actcatctgt ttcgcttaat tttttattgt aaaatcccct atattctttc
     4681 atttctctag tattcatctt ctacctccta tgtttttctt ataagaatat cacaccgtac
     4741 ttatattttc aatattaaat acaaattttc attttatgtt cattagccta atttatatta
     4801 ttttttgaaa tctcaataag ttatcatata agtaagattt tttcagaact aaaatagaaa
     4861 tcttagtaat ttctttatta tctatctcag ttctgaaata tattattctt atccaaacat
     4921 tattatagtg attaattata ttagttattt ttacctgaaa ttttatacat caaataagaa
     4981 tatataaatg gtagtattac aattacaaag attacaaaga tattataaaa aggaatatta
     5041 ataaatgcat taataattaa taatattcca ccaataagcc aaatccatcc agctaaatgg
     5101 tgtgtcttat tccaattatc ttcatcatta agagtccatg gtaaaattat tcctactgag
     5161 taatttcgtt tacatttagg aagatagttt ccaaataaaa taactattat tcctaaaatt
     5221 aatcctgtat aaaaattaga ctccaaacta actcctgttg aatataaaac catcattatt
     5281 tgtataatta ctgaaagtat agcaactccc cattttgata aattttgtat aacatttgaa
     5341 aaattagctt tcttaggctc attctcaagt ctaaaccatg tatacaaatg gatacatagc
     5401 attaacaatg gtacaccaaa agctgctata acctttggag cataattatc tggatttcca
     5461 taattatcaa aatgaattgc aacttcattt ggtaatctat tataaaaata tattgataca
     5521 ataaatggta taaaacatat taaagtagat aaaataaatt gtttatttat cgcttttttc
     5581 attatctttt tctcccttct ccataaattg tgaaaaccaa agcatgacct cttcgaaaac
     5641 tgaaatattt atttcgtaga aaataaagtt cttatacttt gtttcaaata tgagtcctgc
     5701 cttttttagt tgagatagat gataagaaat tgtagcattc gtcatattaa atttttcact
     5761 aatttctcct gctgacattt tattctccct caacataacc aatatttctc ttcttatagg
     5821 gtctgaaaga gctttgaatg tttctggaaa ccccaacacc atcacctcgc tatttagaat
     5881 ttcatctaaa tagaatataa cataacatcc attttatttc aatagctatt tagaaatttt
     5941 tctaaatagt tatttctaaa tatttattga aaatttttat attaagattc tcaatttact
     6001 taagtcgctt ttatttaatc ttactttatc taattttttt aacttaacaa aagtgtattg
     6061 caccataatt gttaaagcaa aaacctaatg attgaggtac ttatatatat aacaatactt
     6121 ttaattttaa attaaaagta gctaaaccca atttaaaaag tagttaagtc ctatttaaat
     6181 agaaaagaat gaaacgagaa atatactgta taaaagtaca taaaaacatc tactaattag
     6241 atacaactat aacagaatat atttattatt ataataacaa aagaataaaa aaaattaatt
     6301 tagcataacc ttgttaaata tagatttgaa tatgaattta aagttatgta aaattaaaaa
     6361 tacaggaatg aaaattccat tcctgtataa aatctaactt ttgagtctca gtacaatctt
     6421 attttgagac aatctttttt gttttatatt ttcttatatt taaaatataa cgcatatctt
     6481 atgcaacata ttcatatttt tctttatatt ctttcatcgc atcattccaa gcgtcttgta
     6541 ctgttacacc tcttttatta gcaatttttt gtgccagttc aattatcttc atagctcttc
     6601 ttgatatgtt cataaacatt cctcctacaa attaccatat atttcatata tataaatcaa
     6661 ggttatattt ttatgatata taatccttct attgtaagat tacagtttat atgtgtatta
     6721 tatatgaact atattagaag ataattagaa tttttataaa gctatatgat tagattaaat
     6781 ataaaagctt tacaattact tataaattta aaaagtctat aagctaaact aatgtttgat
     6841 ataccaattt tacgaccata aataatgcca ttactaaaaa tattggtttt atcatctttg
     6901 aacctttctt tatagcaact ttagcaccta cataagctcc cattatcatc actaatgcaa
     6961 acactatagc aattttataa tctacttgac catttatcat gaataataac aatgaagtta
     7021 tattacttgt taagtttaat atttttgtat ttgcagaagc atgtaaaaaa tcatatccat
     7081 atatttttat aaatccaaaa gtcaaaaaag ttcctgtacc tggaccaaaa aaaccatcat
     7141 aaaaacccat tataagagcc ataagcatcc ctaatcttaa atttttctta ttaacagctt
     7201 caaaattatc ttcttgacct aaatttttag aaataaaggt gtaaatagcc actattaaaa
     7261 tcataaccac aaccaaaaca tttaatatct cttcacttat tgataataca catctcacac
     7321 ctagaacact accaataata gtaaatggta ctaatttttt taaaaggttg ttattaattt
     7381 ttccagactt tgcatatcta taggcactac ttatacatcc tgctgatgct gcaaatttat
     7441 ttgtaccaat ggcaagatgt actggcactc caattgccat aagaacaggc atgcttataa
     7501 gtcctcctcc tcctgctata gaatctacaa aagcagctaa aaacccccct atacataaaa
     7561 aaataactga aaacataaaa ttcctccatc attctctttc aatttttctt acaatatata
     7621 ttatataatc ttatttaata cttattatcc agcatattct ttctatattc atttggagtt
     7681 atactattaa acttcttaaa tatttgtgaa aaataactac tgttattaaa acctactttc
     7741 ttacaaatat cactaatact catgcttcta tctaacagat attttttaga ttcttctatc
     7801 ctaagaagat ttatatactc agttattgaa actttatttc tttcattaaa aatagaactc
     7861 aaataatttg gacttataaa aaaaacatct gatatactat tcaaatttat attttcttta
     7921 taatattttt ttatatattc gtttatattt tcaattagat ttcctttatc ttttctccaa
     7981 aatgctctat tcttatcaat acactctttg gaaaattcaa ataatacatt ccataaatct
     8041 tcatcactgt ttacaagtga tattttttta ttatatttat ttcttaaata agatataagt
     8101 tttaaatcct ctaaatctct aagcactgtt ctagacaata caaatatcat ttcctgaata
     8161 gtatctctaa catcattata tcttaatttg tttgtatcta ttagattata aacaccttct
     8221 aaattcatat ttttaatttc tttttcaata taaagttcta attcaaaggg atattctttt
     8281 gaagaattat tgaacatatc tatcggatta ataatatact cttcttctaa taatctacta
     8341 tttgcttttt taagtgcctc ttcaagtctt atattttctt ttctactctt caatatctcc
     8401 tcatctttta tactaagttg attttcaaaa aaattcctct tagctatttc ctgaataaat
     8461 tcagacatca ttaataaatt ttcttttaat acatataatc tacttttagg atttaatgga
     8521 aattcattgt aaattttgtt tatgtatttt atatcaatac cagaactact tgataattca
     8581 gataaatttt tatctatatc aatagactta tatactataa aattaccaca ttctatatat
     8641 ccaactatat tttcttctaa tactataggt atctctaaac tggaaacacc atactcacaa
     8701 tggtacactt tttcaaagtt atcattattt atgcttttaa caagtttttt agtacactgt
     8761 tttatatttt tacaaatatc gcaaaattta ttttctttct tagtctcaaa tatttcttta
     8821 tactttaagt catatacaga tattctaagc ttcaatatat tagatatttg atttgcaaga
     8881 ttttcaatta tgtttacatt aactaactcc tgtatattgt tttcagaaaa tactataggt
     8941 atttgtctca aactcgaaaa tttatgagga acataagtaa ttaatattac taaatcaatt
     9001 ttaccagtgt ttataatttc atgttctgaa tatggtgggc aataaactat agatgtttcc
     9061 tttatattga cctttttacc atcaattatt tgtattcctt ctccctgaat cacatataaa
     9121 agctgttctt cacttaaatg aaaatgcttt tcttggtagt ttcccggaaa aaatcttaca
     9181 attccagcac taagtctatt gaattgtgtt tttgatggtt cgtgtatcca cataacctct
     9241 ccccaatcaa atttttgtac ttcattattt gactcactaa aatactccat aagctcacct
     9301 cttagttttt gtctaaatat tttttcttcc cttcttatat taaaataata tcatcatcat
     9361 taactttcat tactataaaa tcatcatact cttttaatac tcctgtaatt ttagttgcac
     9421 ttagtttact acaattcagt tttttaatat attgataaat tctataatta cttttttatt
     9481 ttgtaaaatc agtgttttcc caaaataatt atatctataa atgaaagtat ttttactgct
     9541 tacatcaata tatgttatca taattttcct ttttattgca tatataaata actgtatata
     9601 acattgtcca atactatata gaaatacatc taagaaagaa tattattaaa ttgttccaat
     9661 tagtattatt tttcttatag aaataaaaaa actgttggag tcttttagaa aaattatttt
     9721 ttacatacct tttttgctta gatagtctat cttcacagct attttgtata tacactcttc
     9781 tagttcatga ttttatttta atatcttgct tatattgctg aaataattga attcttttat
     9841 ctatcatatt gtctcttata tcacaaacat attgtttctt atatcacaaa aatgaaagca
     9901 ttcttatatc ttcaatatat aaacctgctt tttttaatct tgtaataaat ataggatact
     9961 ctatattctc ttcctagaaa taactaatac cacttatttt tctattatat agaaagtaat
    10021 aaatcttttt cttaataaaa ttcaatagta agtggaataa aattttatat gaataagaac
    10081 attataaaaa tactttatac aatagtcaat aaaagatatt agcttcaaat tatgttaaag
    10141 ttgtattatt taaagtgaaa aaacaaataa aaaagaaata atagtataag tagaaaaata
    10201 tacttaaaac aaggcgaagc ctctcttgta tctaaatcaa gtagagaatt aaatctaaaa
    10261 aagaaaataa atataaaaga tgactcctgt acattacaag aatcatctta tggtttcata
    10321 atcactccat ggtttttaac tgtctgatat aaaggtctca gttcactcat agcatgacca
    10381 ccttttccga ttgctgaccc cgtcacttat ggacaaaatg tccataagtg atagtcctct
    10441 cttaaaaccc tctaaataga tatatctcaa gcaccgcatt gaaaccagtt gctggaaaca
    10501 aactgaagcg ttctggaata gtaaaagaaa ttgccaccat atccacgtta ctgtcctagt
    10561 tgttactttg ctaacttcgg agcgatttcc tctaacaata cgcagtcaaa ttctgccttt
    10621 ttgctaagaa atggcagatg gtctgcatct ggtataaaaa aaatctgttt gtctggcgcc
    10681 actatttcat caaaaaattt cttcgctgga agataggggc tattatagtc attttctccc
    10741 atgatgtagt atacagggac ctgatagtca actccataat ttcttatgtc agcttcttcc
    10801 actaaaaacc gcaatacatc accttgattg tcaatcgcat ttggattgaa aatggccgta
    10861 ttttttaact ctctcaatga ataatacggg gttgccagta tactgattat attgcccaaa
    10921 tccatattta tagaaagccc atacttgctt tggtatgttc ttactgtcat aagagctgcc
    10981 aaatagcttg gaccatattt tgctggcgga tatggtgcaa tagcttccag cgccttgata
    11041 tctttttgct tccctgcagc tttcaccatt tcaagtgtct tttcatagcc cacgcgctca
    11101 ttgtccacca tattaacggt ctgcgcaatc ccaatgtacg cgctgtaatc ctccggatag
    11161 gtttgcacag ccatagttcc cagaactgtt ccccagctat gacctataag gataattttc
    11221 tccttgttga actcttgctt caaatatgct gtcacctcat ggacgtcttg cagaacacgc
    11281 tccgcgctca tggtctgcaa aactgctgcc ggatcgttcg caaagtatgt tttgcctacg
    11341 tttcgctgat cccagttcac cacagtaaaa tcattttccc actcatactg gtacagatgt
    11401 aaaaatggca tcattgaatt accaggaccc ccatgaaccc aaaggatgat ggggttgtct
    11461 gcgttttgtc cccggatata taacgcctgc tgtatgccgc cgatctccac catttccatg
    11521 cggtcgattc caggagcttc tattttatgg cttgtacgta taatcgtggg ggctatcaga
    11581 cgcaaaccgg caaacgccag aacaaaaaca gcaactagta tgcaaagtgt gattaatatc
    11641 attttaagta ccttctttca tgaatttttt tagccataga tatctcctct aacattaaaa
    11701 aaaatatgtt gctattttag ttttgttact ctacctgtcc cattggtttc ctttcataaa
    11761 aaatattaat tgattctata atctcatctt ccaggatatc aactcctatt tgtctcataa
    11821 gaaactgtaa gaattgaaac cgtttacgaa gtgtaggaaa gtccccctga aatacatctg
    11881 tatcacacca aaagttaaag agcagcaaaa ttacctctgc gcattcatct gggaactctg
    11941 tgatgatgct tctatcctct atgccttctc gtaaaacatt tgctaatatt ggtgctagtt
    12001 ttttcaaatt atcctgtgta aatgccagta tgatatgcgg actttctatc gcgcttgtaa
    12061 tcatgttact agattctttt atcacctcat ccgttaaatg ccgcctaact aaacttctaa
    12121 gcttctcttt tgctgtaagt ccatgcattt caccgagcca ttgatttact gtctctatta
    12181 tctgttcaaa gcgcctttcc atgaccacat taaaaatatc ttcttttgaa ctgaagtgat
    12241 gaaaaattcc gccctttgac attcctgaag catcaacaat attttgcata cttgttttgt
    12301 catatccttt ttcagcaaac aactttgcag aaatggtaat gatattttca atagcttgct
    12361 ttggattgtt ctttctagtc attatataac tccttcttat taaaaaccga ctgttggttt
    12421 ttattatata agctattcct gcttctgtca agtaacattg tgtttatggc aaaacaataa
    12481 ggtttggtgg ttagacaggt attaattaga ctaatgcgac tttcccctag atggaaccct
    12541 tggtaaattt gcaatcttta ccggctttga ttatatctgt aagcaaggca ggtatggcca
    12601 cacctgccct gtttgcccct cggatattca ttcctatccc tcctctcctt agccattaca
    12661 ccaaaacgcc gctcattgtc tccaatggcg gcgttttaaa gatatgattt ctcatgctgc
    12721 tgaaatatgc aaaaaatata agatgccctt tattattttt cctacaatag aaaagaaaag
    12781 tattgaagaa tgggttaata ttggagcaaa tatgctttgt ttggcacaag atactacctt
    12841 tatcacttat gttatcaatc aattattatc tttttataat gagattgtac caattcaaaa
    12901 acaacattaa attataaatc taattttata ttaaaagaga ctaatatttt atctatctct
    12961 attaaggata ttttcttatg tatcataata ttagtctctc ttattattat atactgctat
    13021 catcattagt tgctatttat actattttta taatatacaa atttattcag ctaacgtttt
    13081 gcctaattct atacactcat cttttgcttc ttcatttggg tcttccattg ctgtaattcc
    13141 ttctccacca ataattgtag caccagcatt ttgcatacgt tcttcccagt ctctcatcca
    13201 ttcacaattt ccccatccat atgaaccaaa taatccaatc tgtttacctg ataccataga
    13261 ttctaattct gaaacaaatg gctccatata cccctcttct agttgctctg ctcccataga
    13321 tgggcaacct aatgcaaatt tatcttcttc ttttaaatca cttggtttca gtaagctcac
    13381 atctaaaact tctggtgtct taccttttaa cttcacacct tcagccacaa aatttgccat
    13441 tttttcagta tttcctgttc cactccaata aacaatatat attttactca tttttacctc
    13501 ctgtagtatt ttttaattaa tcaatagtta gttatagcta actaacatct aaaaaacaaa
    13561 agatttcttt atctgaaatc tctttatttt tctaaatata gaatatcatg gttgattttg
    13621 aaattcaata cttattgata atatttatca tttatatttt atattcattt tataatatca
    13681 aaacactgta tcgtatttct aatctttcat agaaaaattt ctaacttata gtacttttgc
    13741 atctcttata aatcttttat atgtataagt acatagaaaa acacaccttt tccagatgtg
    13801 ctatctataa tatcgtatat ttatttttca attctaacta gctaatatgt ttataaattt
    13861 tatcacttaa tacctttata cattgatgta aaatcttaca cctaagtaat ttttattatt
    13921 tgtctcgact ctatataata tagctatttt atcatcaaat caaaaattat cttttgctat
    13981 tattcttgta tttataaaga tttaaatata agtatccatt tccttgttcc gttctaggta
    14041 ttccaagcgc tctcgtacat tttattaatt gtgcatacag ttcagcttca tctaaatctc
    14101 tttcaaactc ctctcttgcc cattctttaa ttagtgctaa tgagcctgat acgtatggtg
    14161 cactcatact ggtaccactc ataatagcaa gtttattatc catataagtc gatataatat
    14221 ttcttcctgg agctaccaaa tctattgtag tatttgaatt actaaacttt tcaaccaaat
    14281 agttctcatt tattgcacct acttctatta cctcagcata actggctgga taactatact
    14341 cacttgtact agaatcacca tctccattat tacctgctgc acacactaca gaaatattat
    14401 tttttactgc ttgcatgaca gcatttttta aattcttatc atctttgttt cccccaagag
    14461 acatagatat aatatcaacc ttgttattta cagcaaagtt aatagcatta attatacttt
    14521 gatatgttcc agtaccatct ttatttaatg cttttgctat taataattta caatctggag
    14581 ctactcccat aacttcatta ttataattag atgcagctat aatacccgcc acatgagttc
    14641 catgaccatt aaaatcctca tatatatttt tatttccatt actgtcatca ctaaaatttg
    14701 caccaccaat tatttttcct tttaaaagag gatgagatat atcacaacct gtatctatta
    14761 taccaactac aatattttta ccagtataac cttcatccca cattccccta gcattcattt
    14821 gttttattcc atatgggaat ttattttttg cacctcttag attttcattt atttcatatg
    14881 gaattagttt cattctttta ttcatcatat atcctcctaa aaattttatt gcttataaaa
    14941 atcatgaatt ttattttata ataggtttat aaatcaaatt aaaacctata tctacttatt
    15001 tatatt
//
LOCUS       DAMJVK010000096        14505 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.100, whole genome shotgun sequence.
ACCESSION   DAMJVK010000096 DAMJVK010000000
VERSION     DAMJVK010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14505
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.100"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            165..1076
                     /locus_tag="PC414_003017"
     CDS             165..1076
                     /locus_tag="PC414_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HDF2465397.1"
                     /translation="MKINRLTEIIVILLNKKLVTAKELADRFEVSTRTIYRDIETLSM
                     SGVPVYMTKGKGGGISLIEEYSIDKAILSKKDKESLIVALKTLQATKYPEINSVVNKI
                     GSIFGEQNFSNWIEIDFTEWGSNFNEDDKFTKIKEAILRRNTINFNYVNSLSSQTNRT
                     VEPLKLMYKSKTWYLYGFCKLKDDFRIFRISRIRNLSIKDEVFSRKIIEEVCLNDSKV
                     IKENTITLKLRFKEKMLFRVFDDFNKDLITKNEDDTYDVITEFPIGEWIYGYILSFGD
                     NVEVLEPKDVRDNVITRLRELSKIYSL"
     gene            1251..1748
                     /locus_tag="PC414_003018"
     CDS             1251..1748
                     /locus_tag="PC414_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HDF2465398.1"
                     /translation="MNYEIVEINEKIVIGISKETTNKDGQAVNDIGELWKKFMGKGIY
                     NAIKGKKNDKTIGLYTDYQGDFTSPYSFVACCEVNSNSDKEKNLEELNTNNTVGESII
                     SKVIPAGKYAKFVIVGGQKEVGDFWFEFWQMDFDRTYISDFEEYQCNTFDTKKQEIHI
                     YIGIK"
     gene            2492..2854
                     /locus_tag="PC414_003019"
     CDS             2492..2854
                     /locus_tag="PC414_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDF2465399.1"
                     /translation="MENKILIVDDAIFMRMIIKEALSKSGYNNLIEANDGEEACNIFK
                     YEKPDLMLLDITMPKKDGLEVLREIKKLDSSAKVVMCSAIGQENMIVEAIKLGALDFI
                     VKPFKTETLVKVVDNALK"
     gene            2919..3536
                     /locus_tag="PC414_003020"
     CDS             2919..3536
                     /locus_tag="PC414_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HDF2465400.1"
                     /translation="MINYLELDDVHIDALREIGNIGSGNAITALASMINSNVEVLIPM
                     VKILEYNEATNLLGGPENKVVCILLDMKGDINGMFMFLLDESITQLMLSSLFNKEEAF
                     LDEIEAIEISAIKEIGNIMASSYVNAIASMLNMTISVSIPDICIDMVGAVLNVPMIRF
                     SDVGDKVLFIENKFKMSDNYFTSHILMIPEMSSLREILVRLGLEV"
     gene            3533..4018
                     /locus_tag="PC414_003021"
     CDS             3533..4018
                     /locus_tag="PC414_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HDF2465401.1"
                     /translation="MNREIVVNIADMKIAYRPNVLVTYALGSCVGVCLIDKVAGIGGM
                     LHVMLPYSKDAINIENKCKFADTGINELIKSMENIGANRIYIKAKIAGGAQMFLGSSN
                     SVIASIGHRNVEAVKKTLLGLNIPILAEDTGMNYGRTIRFYTETGELLVDSINKGKKK
                     L"
     gene            4061..4579
                     /locus_tag="PC414_003022"
     CDS             4061..4579
                     /locus_tag="PC414_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-binding chemotaxis protein CheW"
                     /protein_id="HDF2465402.1"
                     /translation="MRFPDDPYLDYGLIDEGIKYLKFKVDEQDFGVELEKVIEITGNQ
                     EIIFIPELPTYSKGIINLRGKIIPIIDMRLRLKKDELMTTNCMYIVVTEIKDLVVGFV
                     VDRVDDIVSLEKSKISPPPRVTIEYSDYFVSGVGELEGKIITLLDLEKLLTDKDEVLI
                     DELINGFDCTIM"
     gene            4715..5674
                     /locus_tag="PC414_003023"
     CDS             4715..5674
                     /locus_tag="PC414_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF2465403.1"
                     /translation="MKNKQQGTILVVDDSVLMCDLIDKTLTKDNFKVKKAFNAIEAKE
                     FIEEFIPDIILLDIILPDNSGFEFCKEVKSNARTADIPIIFITSKNTDTDIVKGFGVG
                     AIDYMVKPFSMTELKARVIAHLEVKKSQDKLKKINNELESSLEKLNRLVVRDYLTGLY
                     NRRHIINQLMEQRRINKYSETSISLILGDIDDFKVINDTYGHEAGDFVLTVISSIIKK
                     NCRQIDTVSRWGGEEFLIMLTNTPIEGAKILSERIRKEIKEFDFNYKEHKIKCTITLG
                     VVEVNSNISLEENISLADKAMYEGKNLGKDCSVVSTMKGLKKI"
     gene            5700..7403
                     /locus_tag="PC414_003024"
     CDS             5700..7403
                     /locus_tag="PC414_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HDF2465404.1"
                     /translation="MKREVKLSKLAIVLLIIIVLAVLAGIFSSISSYKAFEKVEKSIN
                     DMNNLRTQSQYIKDTTREISDVLRRYVFTGEKKNKDIYEKELYGKTRESALNKLEKIG
                     LTKSESNKILEAKEISDSLVPTEQSAIKAVESNDKSKAQSIIFGSDYINSQEKMDSLI
                     NEFQEDIVTRSNSKIVEVKKELQSLILRTIFILSLLIILTIIEYTVIKLKIVIPINKV
                     EKHFSRIASGDLRTSIDVEESKSEIGLLVSSVKKMQSMLLNYIDLIDSTLTSIAKGDL
                     RVEIDQEFIGDFKSIKISLESIINSFNGDFSEINLSAEQVASASEQVAAGAQALSQGA
                     TEQASSVEELASTINEISDGIKKCADSASLACEVSDQSASQVGFGSRCMDDMMTSMGE
                     ISEKSSEISKIIKTIDEIAFQTNILALNAAVEAARAGQYGRGFAVVADEVRSLAGKSA
                     KAAQNTAILIEEAIKTVENGVRIAKETANALDLVVNGVEKTTNYINGISSAVHSQEVA
                     ITQILSGIEQISIVVQMNSATAEESAAASEEMSAQAQILKELVEKFHLKESEVRESSW
                     V"
     gene            7664..9766
                     /locus_tag="PC414_003025"
     CDS             7664..9766
                     /locus_tag="PC414_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HDF2465405.1"
                     /translation="MRQMLDMFIFETSLLLEQLDEIMIQIEKEKVFTHNNINETFRIM
                     HTIKGSASMMGLDNISALAHSVEDLFYAIREGKIEVLDNDLLFDLVFQSLDFIRRDID
                     ELQSRGCISSDYSNFIEGIEEYVSTLELSSSMKSKDNANLESKDEKEVIDSNFEYKED
                     SDVVKIIFEQGCMMENVRAFMLVFELRDYCEFIDFYPKDVETNAESTEYIKSYGFYIQ
                     FVAKEDKAIVYKTIEDNLYVKEYINISKKEIEMKSTSNNDAVENKLKDNNKVSKSYQN
                     NYTEDEIQEDVLNEKTDMEKDSLSEENEHLPISKQSIMSVNLEKLDLLQNIVGEIVIM
                     ESMVVNSVNSTGVDIDNFNKATRQLHKLTDELQDIVMSMRMIQIAGVFQKMHRIVRDM
                     NKKLDKNVELITIGAETEVDKSVIDNLAEPFMHLIRNAMDHAIEPIEERIAKGKEEVG
                     KIVLSAENLGGEAVISISDDGRGIMRDKILAKAKEQGLLKHQSNEYTDDEVNSLIMTP
                     GFSTKESITEFSGRGVGMDIVRKSIEKVGGSIEVKSKQDEGTTFTIKIPLTLSIVEGM
                     KFKVGDSLFTLPISSIRRTFKLTDLNQIITEGNKTELITIYGACIPIIRLHKIYNIQT
                     KVTNLMNGNMIIIENEHKAVCIFVDEIIGNQQVVVKPFPVYFNRFNIKNKGLSGCTIL
                     GDGSISLIIDADSIIEIY"
     gene            9779..10219
                     /locus_tag="PC414_003026"
     CDS             9779..10219
                     /locus_tag="PC414_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HDF2465406.1"
                     /translation="MAKDEIIKQVLTFYVNDVIYGIELENVIETIRFQSITYVPCLPT
                     YISGVINLRGRIIPVINMHIKYNLPKADYNERTCIIITKVDEYQVGIIVDKVVDVIHI
                     ENLNLLETTNSNNTNINKDIKEIAKVEDNSILILDIRKFLINSI"
     gene            10255..11058
                     /locus_tag="PC414_003027"
     CDS             10255..11058
                     /locus_tag="PC414_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HDF2465407.1"
                     /translation="MIKLTDEEFIVLVNHVKKEYGIDLSKKRALIEGRLYNTMIEKKL
                     SSFSQYMNLLFKDRTGNEAINLINRLSTNHTFFMREPQHFEFIQNSILPFWEENNKIR
                     NINIWSAGCSSGEEAYSIAMTLDDYFGYNKELWNIKIIATDISTNSLEKAKRGIYIES
                     NVNNVPELWKKKYFIDNKDGTFKICDKIRKSVIFKPFNLMNDFSYQHFDLIFCRNVMI
                     YFDLKTREELINKFYNATKKDSFLFVGHAEVINRSSTKYKYVKPAIYKR"
     gene            11098..11694
                     /locus_tag="PC414_003028"
     CDS             11098..11694
                     /locus_tag="PC414_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HDF2465408.1"
                     /translation="MERFNNMVIAIGASVGGTEAILEIIKDLPKSTPGIVVVQHMPAI
                     FTYMYAQRLDKQCIMNVREAKNNDRVEQGNVLIAPGGYQMKLCTDKQGYYVTCEKGER
                     ISGHCPSVDVLFDSVAEVAGKNSIGIILTGMGYDGASGLLKMKENGSFTIGQDENSCI
                     VYGMPMVAFDRGSVMLQLPLGEISNCLIRHINILKQKD"
     gene            11800..12663
                     /locus_tag="PC414_003029"
     CDS             11800..12663
                     /locus_tag="PC414_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HDF2465409.1"
                     /translation="MKFFKVDTKAKVLRYVFVLTLTIMAVLAIKHDYKYNNLRKDASI
                     NLNSNKEAVTVMSNQSLPKGNTNSKQTLSVSTIGSKTIVKNDEYLESTINMPVITNSN
                     KIVERSTNDKIKNDIFEFYNKSYKEAKQYLKDNPDEKNKFVANVDFELKKNTDSALSI
                     KVRYYTYSGGAHGFYQDIAYNVDMRTGKFLELMDLFKDNTKYKEVIDEEIKRQIAELE
                     KKDEENIGIYNFKGIKENQNFYLQDENLVVYFDLYDITPYAAGIPEFSINKALISSML
                     KPEYVDLFDLK"
     gene            12730..13719
                     /locus_tag="PC414_003030"
     CDS             12730..13719
                     /locus_tag="PC414_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HDF2465410.1"
                     /translation="MLLLKKDDIKKVFTMRDAIEADKEAFRIYCEGKSVNPLRTNISV
                     PSQDASMLFMPGYVEELGCGGIKIVSVFPKNAQKGKPVIPATVLLLDGETGEVSAVLD
                     GTYVTQIRTGAASGAAIDVLAKKDSKIGALIGLGGQGEPQLEAMIEARNLDEVRVFSR
                     NKESREKFAEEMNLKLSKYNTKIVAVESSDEAIDNADVIVLATPSKQPVLNGNLVKKG
                     ALISAVGSYMPDMQELCPNCLTRASKIFFESTDAVLSESGDILIPLKEGKISKDDFSG
                     DLGNVINGTIPGREDDDEIIVFKTVGIGVQDVVTAKRIYDKAKENGIGIDWKW"
     gene            13811..14422
                     /locus_tag="PC414_003031"
     CDS             13811..14422
                     /locus_tag="PC414_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDF2465411.1"
                     /translation="MKMVKKLILSTIMALVLVLTIGCSNAEEKVKSNFEANLAALEMY
                     KYDEKGISDALTEAEYKQIQTYNSKDKVSMIAQLTPLYGKENAEKIYETIVKLIQGVE
                     KTVTVESSSKDKITLKVVSKALDTSKVMSEANTLVKSYVTDEKNYDKKEKEVQDDMTK
                     MIIDLYNKKPTKEVTTMITYTKKDGKWQPPSNIQKLCEGIYTI"
BASE COUNT         5904 a         1370 c         2695 g         4536 t
ORIGIN      
        1 aataaataaa ttaagatgat aatatccaaa gtgagaaaga taatagaaag taaataaaat
       61 catgtaaaaa taatttagat aagattctaa gattttattt atttaaaaat tagtatgatt
      121 aatttagatt aaataaattt aataatttta gtaggtggta atgaatgaaa ataaatagat
      181 taacagaaat aatagtaatt ctattaaata aaaaattagt aacagctaaa gagttggcag
      241 atagatttga agtttctaca agaaccatat atagagatat agaaactctg tctatgtcgg
      301 gagtaccagt atatatgacg aaaggcaaag gaggagggat atctttaata gaagaatatt
      361 ctatagacaa ggctatattg tcaaaaaaag ataaagagag tttaatagtt gcactgaaaa
      421 cattacaggc gactaagtat ccagaaataa attcagtagt aaataaaatt ggctcaatat
      481 ttggagagca gaatttttca aactggattg aaatagactt tactgaatgg ggaagtaatt
      541 ttaatgaaga tgataaattt acaaaaataa aagaagctat tttaaggcgt aatactataa
      601 attttaatta tgtaaatagt ttgtcaagcc agacaaacag aactgtcgaa ccactgaagc
      661 ttatgtataa gagtaagact tggtacttgt atggattttg taaactaaaa gatgatttta
      721 ggatatttag aattagtagg ataaggaatt tatctataaa ggatgaagta ttttcaagaa
      781 agataataga agaagtatgt ttgaatgatt ctaaagtaat taaggaaaat actataacgt
      841 tgaaattgag gtttaaagaa aagatgcttt ttagagtatt tgatgatttt aataaggatt
      901 taataactaa aaatgaagat gatacatatg atgtaattac agaatttcct attggtgaat
      961 ggatttatgg atatatttta tcatttggtg acaatgttga agtacttgaa cctaaagatg
     1021 ttagggataa tgtaataact agactaaggg agttgtctaa aatttattca ttataatata
     1081 atgctagatt aaagatttta ttaaatatat aatatcttgc atttagtata taatatctca
     1141 ctgagtttaa ttatattaaa aatatgacat aaaggtgtcg tatattataa attataattg
     1201 aattagttca aaataaaata gattaataaa aacaaagtga ggtataaagt atgaattatg
     1261 aaatagtaga gataaatgag aaaatagtta taggaatatc gaaggagact acaaataaag
     1321 atggacaagc ggtaaatgat ataggtgaat tgtggaaaaa atttatgggc aaagggatat
     1381 ataatgctat aaagggtaaa aagaatgaca agacaatagg tctatataca gattaccagg
     1441 gtgattttac aagtccgtat agctttgttg catgttgtga agtaaatagt aattctgata
     1501 aagagaagaa tttagaagaa ttaaacacta ataatactgt tggagaaagt attattagta
     1561 aagttatacc agcaggtaag tatgcaaagt ttgttatagt aggaggtcaa aaagaagtag
     1621 gagatttttg gtttgagttt tggcaaatgg attttgatag gacttatata agtgattttg
     1681 aagagtatca atgtaatact tttgatacaa agaaacaaga aattcatatt tacataggaa
     1741 taaagtagat ttgttattac tatattgaat gaaatattga gtatagtaca tttattatag
     1801 atagttttat tatttataac tataagcagt aaatctgtaa aagatttact gcttttttag
     1861 taattaatag tatcagtatc actttgaatc ttcagacata ttcttcattt aatattgttt
     1921 attgataaat gatacttgta aatataatga tttgttattt aatttaaaat atgaaaaaaa
     1981 gtattataaa cacattagaa tacttctgga tttatgagta tacaatttga aaaatataaa
     2041 aaataatgta taaagctaaa tctaatttaa tcgataatac atatagagaa atataattaa
     2101 gtaaataaac aactatctat tagatattga gaaagtcaaa tcctatatga atctacagaa
     2161 atgattttaa tgaatctgaa aatttttttt atacaaacat aaaaaaggta taaagttgtt
     2221 ttattataat ttaattactt ttgataactt tttatgtttt atacaattat atttttaata
     2281 actcgtcaaa acgccaaaca tatattacaa tagatatata agtttttttg ttgacaaatt
     2341 atgttaagag aattaagtat aatatggcat aaataattac ttttatttat caatgaaatg
     2401 tatttattgt gtagcgaaaa atagtcagat agtatagaca ataactgaag ataaaagcgt
     2461 ttattgttgt acatatggag gagttagttt tatggaaaat aaaattttga tagtagatga
     2521 tgctattttt atgagaatga taattaaaga agctctgagt aaaagtggct ataataattt
     2581 aatagaagca aatgatggag aagaagcgtg taatatattt aaatatgaaa agcctgattt
     2641 gatgttgctt gatattacaa tgccaaaaaa agatggttta gaggtgctaa gagaaataaa
     2701 aaagttggat tcaagtgcta aggttgtaat gtgctctgca ataggacaag agaatatgat
     2761 tgtagaagcc ataaaacttg gagcgttaga ttttatagtg aagcccttca aaacagaaac
     2821 attagttaaa gttgtagata atgctttaaa ataaaagtgt aaatatacac taatacaatg
     2881 aaaagcaaat aaatgcaata ataaagggag gggtttatat tataaattat ttagaacttg
     2941 atgatgttca tatagatgct cttagagaaa ttggtaatat aggctctggg aatgcaatta
     3001 ctgcacttgc atccatgata aatagcaatg tagaagtatt aatacctatg gtcaagatac
     3061 ttgaatataa tgaagcgaca aatttgcttg gtggtccaga aaacaaagta gtatgtattt
     3121 tactcgatat gaaaggtgat atcaatggaa tgtttatgtt tttgttggat gaatcaataa
     3181 ctcaacttat gctaagttct ctttttaata aagaagaagc ttttttagat gagattgagg
     3241 caattgaaat ttcagcaatt aaggaaatag gaaatattat ggcaagttca tatgtaaatg
     3301 ctattgcatc tatgttaaac atgactataa gtgtttcaat tccagatata tgtattgata
     3361 tggttggagc tgttctaaat gtacctatga taagattttc agatgttggt gacaaagtct
     3421 tatttataga aaataaattt aaaatgagtg ataattattt tactagtcac attttgatga
     3481 ttccagaaat gagctcactt agagaaatac tcgtgaggtt gggattagaa gtatgaatag
     3541 agagatagta gtgaacattg cagatatgaa gatagcctat agaccaaatg ttttagttac
     3601 ttatgcactt ggttcttgtg taggagtatg tttgatagat aaagtagctg gaattggtgg
     3661 aatgttacac gttatgttgc catatagtaa agatgctatt aacattgaaa ataagtgtaa
     3721 atttgctgac acaggaataa atgagctcat taaatctatg gagaatatag gtgcaaatag
     3781 aatatatata aaagcaaaaa ttgctggagg tgctcagatg tttttaggaa gtagcaattc
     3841 agtaattgca agtataggac ataggaatgt tgaggcagta aaaaaaactc ttttaggatt
     3901 aaatattcca atattagctg aggacacagg tatgaattat ggtagaacaa tacgttttta
     3961 cactgagacg ggtgaattac ttgtagattc aataaataaa ggtaagaaga aattataata
     4021 tatatttcat agaaagtgta ttttaggagg gaggtattaa atgagatttc cagatgaccc
     4081 atatttagat tatggactaa ttgatgaagg aattaagtat ctaaagttta aagtagatga
     4141 gcaagatttt ggggttgaac ttgaaaaggt aatagaaatt actggaaatc aagaaattat
     4201 atttattcca gaactaccta catattctaa aggcataata aatctaagag gaaaaatcat
     4261 tccgataata gatatgcgtt tgagacttaa aaaagatgaa ttaatgacta caaattgcat
     4321 gtatatagta gtaactgaaa taaaggactt ggtagttgga tttgtggttg atagagttga
     4381 tgatatagta agcttagaaa aaagtaaaat atcacctcca ccaagagtta ctatagaata
     4441 ttcggattat tttgtaagtg gagttggaga attagaaggt aaaataatta ctttactaga
     4501 tttggagaaa cttttgacag ataaagatga agttttaata gatgaattaa tcaatgggtt
     4561 tgattgcact atcatgtaac atagggatgt agttggagtg ttattataaa aagattgtga
     4621 tgagaacatt taagaatata gtggtataat tattaagtat aaaaatttta aatcattaaa
     4681 atttgaatat attatgttta agagaggttt gtatatgaaa aataagcaac agggaacaat
     4741 tttagtcgtt gatgatagtg ttctgatgtg tgacttaata gataaaacac ttacaaaaga
     4801 taattttaag gttaaaaaag catttaatgc aatagaggca aaagaattta ttgaagaatt
     4861 tataccagac attattttgc ttgatataat tttaccagat aactctggtt ttgagttttg
     4921 taaggaagta aaatctaatg ctcgtacagc agatatacct ataattttta taacttcaaa
     4981 aaatacagat acagacatag taaaaggatt tggtgtagga gcaattgact atatggtgaa
     5041 accttttagt atgacagaat taaaagctcg tgttatagct catcttgaag taaagaaatc
     5101 tcaagataaa ttaaaaaaga taaataatga actggaatct tctcttgaaa aattgaatag
     5161 actcgttgta agagattatc ttacaggtct ttacaatagg cgacatataa ttaatcagct
     5221 tatggagcaa agaagaatta ataaatatag tgaaacatca atatccctta tccttggtga
     5281 tattgatgac tttaaggtta tcaatgatac ttatgggcat gaagcagggg atttcgtgtt
     5341 aacagtaata tcaagtataa taaaaaagaa ttgtagacaa attgatactg tatcaagatg
     5401 gggtggagaa gagtttttga taatgctcac aaatactcca atagaaggtg ctaagatact
     5461 ttcagaaaga atccgtaaag agataaagga gtttgatttc aactataaag aacataaaat
     5521 aaagtgcact attacattag gagtagtaga agtaaactca aatatatcat tagaagaaaa
     5581 catatcctta gcagataaag caatgtatga gggtaaaaac ttaggaaaag attgtagcgt
     5641 agtgagtaca atgaaaggat taaaaaagat ataaacttac tttatgaagg agtacagata
     5701 tgaaaagaga agtaaagctt tctaaattag caattgtatt gctgattatt atagtattgg
     5761 ctgttctagc agggattttt tcttctattt catcttataa agcatttgaa aaagtagaaa
     5821 aaagtattaa tgatatgaat aatttgagga ctcaaagtca gtatataaaa gatacaacta
     5881 gagagataag tgatgtatta agaagatatg tatttacagg agaaaaaaag aataaagata
     5941 tttatgaaaa agaattatac ggaaaaacaa gagaatcagc tttaaacaaa ttagaaaaaa
     6001 ttggtcttac aaagagcgag tcaaataaaa tcttagaagc aaaagaaata tcagattctt
     6061 tagtaccaac agaacaaagt gcaataaagg cagtcgaaag taatgataag agtaaagctc
     6121 aaagtataat ttttggtagt gactatataa actcccaaga gaaaatggat tctttaataa
     6181 atgaatttca agaggatata gttacccgtt caaattccaa aattgtagaa gtaaaaaaag
     6241 aattacaaag tctaatatta agaacaatat ttatactttc ccttttaata atattgacaa
     6301 taatagaata tacagtaata aaattaaaaa tagtgattcc tattaataaa gtagaaaaac
     6361 acttttcaag aattgcatct ggggacttaa gaacaagtat agacgttgaa gaaagtaaat
     6421 ctgaaatagg gctattagtg agttctgtca aaaagatgca aagtatgctt ttaaattaca
     6481 tagaccttat agacagtaca ttaactagta ttgcaaaagg agatttaaga gtagagatag
     6541 accaagaatt tataggtgat tttaaatcaa ttaaaatatc tttagaaagt ataataaact
     6601 cttttaatgg tgatttttct gaaataaatt tatcagcaga gcaagttgca agtgcttcag
     6661 aacaagttgc agcaggtgca caagctcttt cacagggtgc tactgaacaa gctagttcag
     6721 tagaagaatt agcttccacg attaatgaga tatcagatgg aattaagaag tgtgcagata
     6781 gtgctagtct tgcatgtgag gtatctgacc aatctgcatc acaagttggt tttggaagta
     6841 gatgtatgga tgatatgatg acatcaatgg gtgaaataag tgaaaaatcg agtgaaatat
     6901 caaaaataat caaaacaata gatgagatag cattccaaac taatattctt gcattaaatg
     6961 cagctgttga agcagcaaga gcaggtcaat atggtagagg atttgcagtt gtagcagatg
     7021 aagttaggtc tttggctggt aaaagtgcaa aagcagctca aaatacagct attctcatag
     7081 aagaagctat taaaacagta gaaaatggtg tcagaattgc taaagagact gcaaatgcat
     7141 tggacttggt agtaaatggt gtagaaaaaa ctactaatta tattaatggt atttcttcgg
     7201 ctgtacatag tcaagaagta gcaattacac agattttatc tggaatagag caaatttcca
     7261 tagtagtcca aatgaactca gcaacagcag aggaaagtgc agcagcctca gaagagatgt
     7321 ctgctcaggc tcaaatttta aaagaattag tagaaaaatt tcatctaaag gaaagtgaag
     7381 taagagaatc atcatgggtt taaattatga ggtgaatatt gaagtaagag aattatcatg
     7441 tatttaaatt atgagatgta tatatattga aataagagaa ttatcaagga tttaaattat
     7501 gagatgtata tatattgaag taagagaata attgtgtatt taaattatga ggtacatata
     7561 gtgagataag agaatcatca aggatttaaa ttatgagatg catatataat agaaaattat
     7621 ttgtaggtcg taggtgataa atgtgaatta tattgaaccg agtatgagac aaatgttaga
     7681 tatgtttata tttgagacaa gtttattact cgaacaatta gatgaaatta tgattcaaat
     7741 agaaaaagag aaagtattta ctcataacaa cattaatgaa acttttagaa taatgcatac
     7801 gataaaaggt tcagcatcta tgatggggtt agacaatata tctgctttag cacatagtgt
     7861 agaagacttg ttttatgcta tacgtgaagg taaaatagaa gttttagaca atgacctatt
     7921 atttgattta gtctttcaat cattagattt tataagaaga gatatagatg agttacaatc
     7981 aagaggttgt atatcttctg attactctaa ttttattgaa ggaattgaag agtatgtgag
     8041 tactttggaa ttatcaagtt ctatgaaatc aaaggataat gcaaatttag aaagtaaaga
     8101 cgaaaaagaa gtaatcgatt caaattttga atacaaagaa gactctgatg tagtaaaaat
     8161 aatctttgaa caaggttgca tgatggaaaa tgtgagagct ttcatgcttg tatttgaact
     8221 tagagattac tgtgaattta tagattttta tccaaaagat gtagaaacaa atgcagagtc
     8281 tacagagtat attaaaagtt atgggtttta tatacaattt gtagctaaag aggataaggc
     8341 aatagtttat aaaactatag aagataatct ctatgtaaaa gaatacataa atattagtaa
     8401 aaaagaaata gaaatgaaaa gtacatcaaa caatgatgca gtagaaaata aattaaaaga
     8461 taataataaa gtaagtaaaa gttatcaaaa taactataca gaagatgaga tacaagaaga
     8521 tgttttaaat gaaaaaactg atatggaaaa agatagtcta agtgaagaaa atgaacattt
     8581 accaataagt aagcagagta ttatgagtgt aaatttagaa aaattggact tacttcaaaa
     8641 tatagttgga gaaattgtaa ttatggaatc aatggttgta aatagcgtaa attctacagg
     8701 tgttgatata gataatttca ataaggcaac aagacaatta cacaagctta cagatgaatt
     8761 gcaagatata gtaatgtcta tgcgtatgat tcaaattgct ggtgtatttc aaaaaatgca
     8821 tagaattgta cgagatatga ataaaaaatt agataaaaat gtagagttaa taactatagg
     8881 agcagaaaca gaggtggata agagtgtaat tgataattta gcggaacctt ttatgcacct
     8941 tataagaaat gctatggacc atgcaataga gcctattgag gagcgtatag cgaaaggtaa
     9001 agaagaagtg ggaaagatag ttttatctgc tgaaaatcta ggcggagaag ctgtgataag
     9061 tatttcagat gatggtagag gcattatgag agataagatt ttggcaaaag ctaaggaaca
     9121 aggtcttctt aaacatcaaa gtaatgagta tactgatgac gaggtcaatt ctttgattat
     9181 gactccaggc ttttctacaa aagaatctat aactgaattt tctggaagag gagtaggcat
     9241 ggacattgtt cgtaaaagta ttgaaaaagt tggaggttca attgaagtaa aaagtaaaca
     9301 ggatgagggg acaaccttta ctataaaaat accattaact ctttctatag tagagggtat
     9361 gaagtttaaa gttggagatt cactatttac tttaccaata tcatccataa gaagaacatt
     9421 taaacttaca gacttaaatc aaataattac agaaggaaat aagacagagt taattacaat
     9481 atatggagca tgtattccca ttataaggtt acataaaata tataatattc aaacaaaagt
     9541 aaccaatctg atgaatggta atatgataat tattgaaaat gagcataaag cagtatgcat
     9601 ttttgttgat gaaataatag gtaatcagca agttgttgtg aaaccatttc cagtttattt
     9661 taaccgattt aatataaaaa ataagggttt atcgggatgt acaatacttg gggatggaag
     9721 tataagttta attatagatg cagatagcat tatagaaata tattagggga tttaaagtat
     9781 ggctaaagat gaaattatta aacaagtatt aacattttat gtcaatgatg ttatttatgg
     9841 aatagaatta gaaaatgtga ttgaaaccat aagatttcaa tcaataacgt atgtaccatg
     9901 tcttccaaca tatataagtg gagttattaa tttgagagga agaataatac cagttattaa
     9961 tatgcatata aagtacaatc ttcctaaagc tgattataat gagagaactt gtataataat
    10021 tacaaaggtt gatgagtatc aagttggaat tattgtagat aaagttgttg atgttattca
    10081 tatagaaaat ctaaatttac tagagacgac taattctaac aacactaata ttaataaaga
    10141 tataaaagag attgcgaaag ttgaagataa ttctatatta attttagata ttcgaaagtt
    10201 tttgattaat agcatatagt tagttctgat aaattagcag gaggaagttt ttgcatgata
    10261 aaactcacag atgaagaatt tatagttctt gtaaaccatg taaaaaaaga atatggtatt
    10321 gatttaagta aaaaaagagc gttaattgaa ggtcgcttat acaatacaat gatagagaaa
    10381 aaacttagtt cttttagtca atatatgaat ctattattta aagataggac aggaaatgaa
    10441 gctataaacc taataaatag attgagtact aaccacacat tttttatgag agaacctcaa
    10501 cattttgagt ttatacagaa tagtatactt cctttttggg aagaaaataa taaaataaga
    10561 aacataaata tatggagtgc aggatgttct agtggagaag aagcgtattc aatagctatg
    10621 actttggatg attattttgg atataataaa gagttgtgga acattaaaat catagccaca
    10681 gatatttcaa ctaactcact tgaaaaagct aaaagaggta tatacataga atcaaatgtt
    10741 aacaatgttc ctgagttatg gaagaagaag tattttatag acaacaagga tggtacattt
    10801 aaaatttgtg ataagattag aaaaagtgta atttttaagc cgtttaattt aatgaatgat
    10861 ttctcatatc aacattttga tttgattttt tgtagaaatg ttatgattta ttttgattta
    10921 aaaacaagag aagaacttat aaataaattt tataatgcta ccaaaaaaga tagttttttg
    10981 tttgtaggac atgcagaagt aatcaacaga agtagtacta aatacaaata tgtaaaacct
    11041 gctatatata aacgataaaa aggtattttt gatatatgat tttttggagg cggtaatgtg
    11101 gaacgtttta ataatatggt tattgctata ggcgcatctg taggtggtac agaggcaata
    11161 cttgaaatta taaaagacct accaaaatca acaccaggga ttgttgtagt tcaacatatg
    11221 ccagctatat ttacttatat gtatgcacaa cggcttgaca agcagtgtat tatgaatgta
    11281 agagaagcta agaataatga tagagtagaa caaggaaatg tgttaattgc acctgggggt
    11341 taccagatga aattgtgtac ggataaacaa ggatattatg taacttgtga aaaaggagaa
    11401 agaataagtg gtcactgtcc atcagtagac gttttgtttg attcagtagc agaagtagca
    11461 ggtaaaaatt caataggaat aatacttaca ggtatgggtt atgatggagc aagtggactt
    11521 ttaaaaatga aagaaaatgg ttcatttaca attggacagg atgaaaattc ttgtatagta
    11581 tatggaatgc caatggtagc ttttgataga ggttctgtta tgttacaatt acctcttggt
    11641 gaaatttcaa actgtttgat aagacatatt aatattttaa aacaaaaaga ttaatttttt
    11701 ataaataaaa attaatcttt tattaaatta tatggataaa aaaccctaga aactccaaat
    11761 ataataagag agatttatat ttaggaggaa aatgataata tgaaattttt taaagtcgat
    11821 acaaaagcta aagttttaag atatgtattt gttttaacat tgactataat ggctgttttg
    11881 gcaattaaac atgactataa atataataat ttaaggaaag atgcttcaat taatttaaat
    11941 agtaataagg aagcagtgac tgttatgagt aaccagtcat taccaaaggg taatacaaat
    12001 agtaaacaaa ctttatcagt aagtacaata ggaagtaaaa caatagtgaa aaatgatgag
    12061 tatttagaaa gtactataaa catgcctgta ataacaaatt caaataaaat tgttgaaagg
    12121 tctacaaatg ataagataaa aaatgacata tttgaatttt ataataaatc atataaagaa
    12181 gcaaagcagt atcttaagga taatcctgac gagaaaaata agtttgttgc aaatgtagac
    12241 tttgaactta aaaagaatac tgattctgct cttagtataa aagtgagata ttatacttat
    12301 agtggtggag cacatggatt ttatcaagat atagcatata atgtggatat gagaactgga
    12361 aaatttttag aattaatgga cttgtttaag gataatacta aatataaaga agttatagat
    12421 gaagaaatta aaagacaaat agctgaactt gaaaaaaagg atgaagaaaa tatcggaata
    12481 tataatttta aaggtataaa agagaatcaa aacttttact tgcaagatga gaatctagtt
    12541 gtatattttg atttgtatga tataactcca tatgcagcag gaataccaga attttcaata
    12601 aataaagcgt tgataagttc tatgctaaaa ccagagtatg tagatttatt tgatttgaaa
    12661 tagaagatta aattaaatgt tatgctataa tatttagcat agagacttat taaaattggg
    12721 ggtataaaaa tgctactttt aaaaaaagac gacataaaaa aagtatttac aatgagagat
    12781 gcaattgaag ctgataagga agcttttaga atttattgtg aaggaaagag tgtaaatcct
    12841 ttgagaacca atatatctgt tccaagtcaa gatgctagta tgttatttat gccaggatac
    12901 gtagaagaat taggctgtgg gggaataaaa attgtatctg tatttcctaa aaatgctcaa
    12961 aaaggcaaac cagtaattcc agcaacagta ctattattag atggagaaac tggagaagtt
    13021 agtgcagtat tggatggtac ttatgtaact cagattcgta ctggagcagc ctctggagca
    13081 gctatagacg tattggctaa aaaagattct aagattggag ctttgatagg tctaggagga
    13141 caaggtgagc cacaacttga agccatgata gaagctagaa atttggatga ggtaagagtt
    13201 tttagtagaa acaaggaaag tagagaaaaa tttgcagaag aaatgaactt aaaattaagt
    13261 aaatataata ctaaaatagt tgcagttgaa tcatctgacg aagccataga taacgcagat
    13321 gtgatagttt tagctactcc atcaaaacaa ccagtactta atggaaactt agtgaaaaaa
    13381 ggagctttaa taagtgcagt aggttcatat atgccagata tgcaagagtt gtgcccaaac
    13441 tgtttaacta gagcgtctaa gatatttttt gaatcaacag atgcagtttt atctgagtca
    13501 ggagatatat taattccatt aaaagaaggt aagataagta aagatgattt tagtggtgat
    13561 ttaggaaatg ttataaacgg aactattcct ggaagagaag atgatgatga gataattgta
    13621 tttaaaactg ttgggatagg tgtacaagat gtcgtaacag caaaaagaat atacgataaa
    13681 gcaaaagaaa acggtattgg aatagactgg aagtggtaaa atattcaaaa taattctata
    13741 gttagacaaa tttatgttat aatgttgata aataaatttt ttattatttt aattgaggga
    13801 ggatgtttgt atgaaaatgg taaaaaagtt aatattatct acgattatgg ctctagtttt
    13861 agttctaact atagggtgtt ctaatgcaga agaaaaagta aagtcgaatt ttgaagctaa
    13921 tttagctgca ttagaaatgt ataagtatga tgaaaaagga atttctgatg cattaacaga
    13981 agctgaatat aaacagattc aaacttataa cagcaaggac aaggtttcaa tgattgctca
    14041 attgacacct ctttatggta aagaaaatgc tgaaaagata tacgaaacaa ttgttaaatt
    14101 aattcaaggt gttgaaaaga cagtaacagt ggaatcttct agtaaggata agataacttt
    14161 gaaagttgta agtaaagctt tagatactag taaagtaatg agtgaagcga acactttagt
    14221 aaaatcttat gtaactgatg aaaagaatta cgataaaaaa gaaaaagaag ttcaagatga
    14281 tatgacaaaa atgattatag acctttacaa taagaaacca actaaggaag ttacaactat
    14341 gattacatat actaaaaaag atggtaaatg gcaaccacct agtaatatac aaaagttatg
    14401 tgaaggaata tatacaattt aatttatttt aaagacacaa aactatcttt taagcatata
    14461 ataaaatgtt tggaagatag tttattttat tttcaaagta taaat
//
LOCUS       DAMJVK010000097        14414 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.101, whole genome shotgun sequence.
ACCESSION   DAMJVK010000097 DAMJVK010000000
VERSION     DAMJVK010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14414
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.101"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            <1..995
                     /locus_tag="PC414_003032"
     CDS             <1..995
                     /locus_tag="PC414_003032"
                     /inference="COORDINATES: protein
                     motif:HMM:NF025159.1,HMM:NF037393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4179 domain-containing protein"
                     /protein_id="HDF2465412.1"
                     /translation="KKKMNRRKSITVTLISFFILGLIALNSETTWAYIRDTTKQIESF
                     FGREKNIFDKYKFEGEQIIESNGLKFSLGEVILDDRQLIVSMSADYTDFKIKFKNIGK
                     VGISPSFPTIYIDDLVFPGQSYSTITEKVKGEKKYNILLKVSLLQIDTNKDGIADTEY
                     EILNKINSNKNYSLKISFTDIDYGIKNGIENNVGNWKFNTTINASNIIKDTKVYEVDK
                     EIKIDEKEYKGTLNIKEVRVSPISIKVKYNYDSYTEIGPAKRSDPYLIIKDENGKKLN
                     NGTGAGGILQNKKNYINGEFELRGSEKKITIIPCIYVNDKEKCLFNKTVNLNID"
     gene            complement(1050..1643)
                     /locus_tag="PC414_003033"
     CDS             complement(1050..1643)
                     /locus_tag="PC414_003033"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465413.1"
                     /translation="MNKDKFNELDISEQIEYINNQLENNETLTYICKNIGIGRSTVRD
                     RFKKANYSYSKDLNKYIHECITDVLQEPSIKNNRCITTDIQKEKNNISNTNITQSDAV
                     REVINKSDKEIKDNLLDIVSNYDVLKEIIELHKCNTSVIKQQIVIDLEDSDSKLTTLR
                     VNSKELELFNRFCDDNKYFKKIDLISQALKEFREKYS"
     gene            2659..2940
                     /locus_tag="PC414_003034"
     CDS             2659..2940
                     /locus_tag="PC414_003034"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VbhA family protein"
                     /protein_id="HDF2465414.1"
                     /translation="MKDYSIKNTTKEQRKEIVNSALGLGSIDSHEPSIVVLELLDRYV
                     EGELEIKEIQDIVLNSYKRKYLDGDEEGIEKMMDEIIDQNIDALKKLSE"
     gene            2955..3179
                     /locus_tag="PC414_003035"
     CDS             2955..3179
                     /locus_tag="PC414_003035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465415.1"
                     /translation="MYIVAKKIDKVGCIASEVESGERIGALIEYLSLTMLDKGIEIVV
                     LDKPNMYGEYAPYEFLSEVEFVCSVLSMSH"
     gene            complement(3399..3665)
                     /locus_tag="PC414_003036"
     CDS             complement(3399..3665)
                     /locus_tag="PC414_003036"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465416.1"
                     /translation="MDDKRNPLLKQLIVIEEFYGEEKITDIKKFIEFLKNNNLEFINL
                     QGCSIEINLKKEMISIIQNITNERVLEFTYEYLKQIDNKTKTYI"
     gene            3877..4068
                     /locus_tag="PC414_003037"
     CDS             3877..4068
                     /locus_tag="PC414_003037"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465417.1"
                     /translation="MLFSNREKRDKYIQIRLTETEKQNLKDIIDRLNEDSSKKISLTD
                     FILNAIDYYIEHECKNYKK"
     gene            4088..5128
                     /locus_tag="PC414_003038"
     CDS             4088..5128
                     /locus_tag="PC414_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HDF2465418.1"
                     /translation="MKGIKKLTSLGLVGLLFSMSVFHSNAFTLERIQGKNKYETAGLV
                     ADKQQYTTAILINADSTMSDGLSASGLAGVNNAPILLTQKNNIPKETLERVERAKKVY
                     IIGGENSIDKSTEGILKDKGKEIKRLQGDDRIKTSYSVAKEINAIKKVNTIMLTNAFK
                     GEVDTMSIAPIAIMKNAPIILTNGSTIPFKFDKTITSYVVGGNKSMTDDLLIKTNSQR
                     LTGVDRYATNRNIIKSFYNRAVEEFYIASGTDLVYPLIASTIVKDKPMVLVNESNNKE
                     ILRNASKITVVGDLDESIIKKCLDVKDGKSETKTKKEKIYVEGFGWVEPTDSEGEEGN
                     SDGDFNKIVANM"
     gene            5495..6073
                     /locus_tag="PC414_003039"
     CDS             5495..6073
                     /locus_tag="PC414_003039"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465419.1"
                     /translation="MKFKEIKKKNYVIGGVLLIMISVSIFIFKDSSNKGESLTNQVKN
                     ETGHKIKEEVKNVDVPKINMDEGSSESKMKMAEKINKENNITQEDYRNFREGIEKKEN
                     EPIKKPNKPNKPNKPNSNIINPEVKPSNNENSVKNNNPEENKNNESENKSEENKKEKV
                     YVEGFGWVEPSESQGKEGTSNGDFNKVVGQMD"
     gene            6093..7868
                     /locus_tag="PC414_003040"
     CDS             6093..7868
                     /locus_tag="PC414_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008423834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465420.1"
                     /translation="MKKRLLIVFLCVTLFIPILVFADGSGNVDGGGGSTGSGGDKKNY
                     WTAGDEGVRITIIDNKTQKPVSTPIDFTNRNINVKVHFGKVSKISYREGKTLNLDSGK
                     YNYINPKNKLPVIISSKTYGKSDIKKIKEYFCNDWTQRIIAKEANFNYEEMQNGEYKL
                     LLEPVAYFTFNGNKMAMTATEAALYNKKLGGNKLRNKFASISHKNLPLAMFLEKDDLG
                     FKAWKGISDTDKAKAKDDDTIIKMLGLGIVYFNNEPTKDDEVTIPPVEPSTPENPDKP
                     SIDGKEYEYHTDTDVISSVKIKGSNRSPDNPVTVKFKIKNQTYNMNNIYLPEEGGEQY
                     AWVKWHTPKTPEKIIIEVSSNATTDKAKIIADVVELEEKTPPDPVAKDKRPNNFKVPT
                     LPKKEETKTLEWGKWDCNWKAVWVYYSDGEGGGEWVDEGYWVWKWIPYSATINATMDT
                     IPSEHCMTQKKTRDVYEMKSAYGIETLVNSKITFNAPEDSVTGGQNVIVTFPEFGYEK
                     YNRVLQNTKIGYDSSFEFKKNKYSTYNAKTHFTPLWYLDGKYSTYATVIDSWTPAGQL
                     KTNIYNDKITIKGNVYDDWHIGPVK"
     gene            7890..9299
                     /locus_tag="PC414_003041"
     CDS             7890..9299
                     /locus_tag="PC414_003041"
                     /inference="COORDINATES: protein motif:HMM:NF013258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HDF2465421.1"
                     /translation="MAFFVYHLAKYSKNNIVGIQKHNQRQNKSYKNKDIDMSKSKLNI
                     DFENKDRIKYLEKVEKIIKENRTKGKVIRKDAILMCESVISASPEFFLGMEQSEKEKY
                     FKYSYEFLKDRYGEKNVISATVHFDETTPHMHFSFVPITSDGKLSARDLITRIELKKI
                     QDELPIYLQSKGFDIKRGREHSEARHMQPEDYKKQQIQELRELENKLEYVKKLFKGFE
                     KPLNSFNELEPANIKKNLITGKETVYKNDYDEVIDILKGTMKENLELKEKLHNEQLKT
                     RNLEVKLNTVNEYERLTHEKEKKLKLEKEEFEEQKQLISEREKNIEKVLENKDKNNKK
                     ILDEKEVEINELEKQYKNLSRKYNNTIREYEKVIEEFSNNDIYIIFNKSSTKEIQEND
                     TYKFKDANNFIKELNKMCFTNNETREIGFQMSFGINNGVFMEKNIILGQYRDMDLKRE
                     IISINKSNNKVLSKDLFRY"
     gene            complement(10086..10730)
                     /locus_tag="PC414_003042"
     CDS             complement(10086..10730)
                     /locus_tag="PC414_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF2465422.1"
                     /translation="MIIVYERVSTRKQDNSRQERVLENEGIKFDKIYRDKITGATKER
                     KALNRMLLEVNEGDVVYCESISRLGRNLKDLIDIIDTLVQKGVMVKILKEGIDTGNNT
                     YKLLLGIFGAVAEMERETIQERTQQKIDQLKEDKKKGEINTKTGKWFGRQKVTKELLE
                     TNKEFIKYYNKILDGNLKKNEVAKILNIGRTTLYEWIKIFEGGEIKRSNRSDKN"
     gene            complement(10936..11373)
                     /locus_tag="PC414_003043"
     CDS             complement(10936..11373)
                     /locus_tag="PC414_003043"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465423.1"
                     /translation="MKLKLKKSFIFITTLSFIFTNLSISLPVFADSLQENIMTIQEPR
                     YTNISKATSELSISGSNAIIKTTLLGKLGITKTTITSTLQKKVNNNWQTVDSWTVSKN
                     DKLCTLSKTKSISKGTYRVSSVVIAYKGSTSERDTLISASKTY"
     gene            complement(11436..12137)
                     /locus_tag="PC414_003044"
     CDS             complement(11436..12137)
                     /locus_tag="PC414_003044"
                     /inference="COORDINATES: protein motif:HMM:NF025643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4367 domain-containing protein"
                     /protein_id="HDF2465424.1"
                     /translation="MNDFKITDEELRKALENVELILISKVPKEDDIDYTFSKSFENKM
                     EKLIKQETKKTLRKNTYSYSKNIVAALLIIIIGLFAMTMSVDAMREQFFNVIKEVYTK
                     FTTYKFSINENIDIGKFNELNLSYLPKGFKEINKTNISNETFVFFSNDDGDEIVFNYS
                     KIADTTMKIDTEEAIIENVKINGINAEYIIKNNDFSLIWNDNTYSYYITLSYIEYIKL
                     QDTKNEIIKIAENIK"
     gene            complement(12140..12670)
                     /locus_tag="PC414_003045"
     CDS             complement(12140..12670)
                     /locus_tag="PC414_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HDF2465425.1"
                     /translation="MILYLNLLESENDISKFELLYNTYKKTMFYIANEILKDECLAED
                     AVHESFLKIIKNLNKINDIKSYKTKAFVIIIVKNTSKDIYRKRKRENEKIKSLEKEFQ
                     SDKFHIDEYSIGYLEEAIMKLSEKHRDVLLLKLQHELNDKEISQILDIKPENVRKRLS
                     RAREELKYIFIKDVGV"
     gene            12810..13577
                     /locus_tag="PC414_003046"
     CDS             12810..13577
                     /locus_tag="PC414_003046"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465426.1"
                     /translation="MFLKKLKKSTVIALTGLMLISSTGNLAFALDNKNEGAKNVSENS
                     IVEPRVVSNSKTFSVGGKSYTNYTSANITLSSGAYGSTTIKSNSGAVGENRLYAHANL
                     YNKNGQVVKTADWKSNGSSTSTLTNTTGNSFSKGTFYTKGTTKVYNGNGYTTQSANQS
                     PNVVVNNLGTRISKEELKERMDLYEIKNMLPAVGIDNISGYISMDDLYDEPNFPETPE
                     EAITYQKIRAKASYRMIPLYDNDGKTIIGEYRIDSAN"
     gene            13774..14352
                     /locus_tag="PC414_003047"
     CDS             13774..14352
                     /locus_tag="PC414_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2465427.1"
                     /translation="MRKLSSFKNLINQNQNDNDNMVISAINGNKHAIEQLINEQKEYL
                     YKTAFLYTKDENEAMDICQETIYKTILNIHKLREPSCFKTWMTKILINNVNEVNRNKI
                     KIFDNDFQIELIKDISYEKLEEKLDLYSAIDLLEEKFKTPIIFQYFQDMTIKEIAEVL
                     ECNENTVKTYLRRGKKKLYEILMRGEIDEKVR"
BASE COUNT         5727 a         1670 c         2098 g         4919 t
ORIGIN      
        1 aaaaaaaaaa aatgaataga agaaaaagca taacagtaac attaataagc ttttttattt
       61 taggattaat agctttaaat agtgaaacaa cctgggcata tataagagat actactaaac
      121 aaattgaatc tttttttggg agagaaaaaa atatttttga taaatataaa tttgaaggtg
      181 aacaaattat tgagagtaat ggattaaaat ttagtttagg tgaagtaatt ttagatgata
      241 gacaattaat agttagtatg agtgctgatt atacagattt taaaatcaag tttaaaaata
      301 taggtaaagt aggcatttct ccttcattcc caactattta tatagatgac ttagtatttc
      361 caggacagag ttattcaaca ataacagaaa aggtaaaagg agaaaagaaa tataatatac
      421 tattaaaagt tagtttattg caaatagata ctaataaaga tggaatagca gatactgaat
      481 atgaaatttt aaataaaatc aattctaata aaaattatag tttgaaaatt tcttttacag
      541 atattgacta tggtataaaa aatggaattg agaataatgt aggtaattgg aaatttaata
      601 ctacaattaa tgcatcaaat ataattaaag atactaaagt atatgaagta gataaagaaa
      661 ttaagataga tgaaaaagaa tacaaaggga cattgaatat aaaagaagtt agagtatctc
      721 caatatcaat taaagttaaa tataattatg atagttatac agaaattggt cccgctaaaa
      781 gaagtgatcc atatttaatt ataaaggacg aaaatggaaa aaaactaaat aatggcactg
      841 gagcaggagg aattcttcag aataagaaaa attatataaa tggggaattc gaactaagag
      901 gaagtgagaa aaaaataact ataatacctt gtatatatgt taatgataaa gaaaaatgcc
      961 tttttaataa gactgttaat ttaaatatag attaaatcaa aataaaacca taggttatta
     1021 acctatggtt ttattttgat ttaataattc taagaatact tctctctaaa ctcttttaag
     1081 gcctgactaa ttagatcaat ttttttaaaa tatttattat catcacaaaa tctattaaat
     1141 aactctaatt ctttactatt aactcttaga gtagtaagtt tactgtcaga atcctctaaa
     1201 tctattacta tctgttgctt tataacggac gtattacatt tgtgtaactc tataatttct
     1261 tttaatacat cataattaga aactatatct aacaaattat cttttatctc tttgtcagat
     1321 ttattaatta cttctcttac tgcatccgat tgtgtaatat ttgtattact aatgttattt
     1381 ttttcttttt gtatatcagt cgtaatacat ctgttatttt ttatgcttgg ctcttgtaat
     1441 acatctgtta tacattcatg tatatacttg tttaagtctt tggaataact gtaattagcc
     1501 tttttaaatc tatcccttac tgtacttctg cctattccta tatttttaca tatgtatgtc
     1561 aaggtttcat tattctctaa ttgattattt atatattcta tttgttctga tatatctaat
     1621 tcattaaatt tatccttgtt catattacac ccccacaatt ataacagatg tataacgtct
     1681 gttatttgaa attatatcat aatattattt taaataaatt tggagtttta aaatataatt
     1741 agcaaaaatt acaaaaaata tgttgttata tcctcagaaa gaatttatac ttaaatatat
     1801 aaaaactaaa taaaaaagca cttacaaaaa gtaagtgctg tctgtaactt cagttcttta
     1861 aatcgcctag gaaacgattt aaggtaacaa ctggtataca aagtaccaag aagttaagga
     1921 ataatccaat cgcaagatag gctaaatcta tcaaggagag ttgacccaac tgaaattatt
     1981 tacaaaatat aattatagta tataaagtaa aataatttta gtcaaggaga aatgttgtac
     2041 acctaaagta tagatataga ctttaggtgt tttttaatgc aataaatgag gttatatgtt
     2101 acctatatga catatagtgg gacagccctt aaccacttaa aaaaaaggtc aggtgagttc
     2161 agccaatgca taaagtgaac ctctgtacaa tgtagctaat ccctttttgt agctagtaca
     2221 ggattgagtg gtgacgacaa cctaaaggtc gggtgtggga gttctaggag cataagtgct
     2281 tgggaactat gaacagaatc attgcattag gatagaacca taatgtagaa agtttatagg
     2341 tgtttacacg taataaaaag ggtacagatg ataaggtgac cgaagtatac ggtttataga
     2401 tttgtaatta cttccttatg atagtcaatt taaaagtaat gactattata agggaataat
     2461 tacgtccaat ttcagttccc tcgtgccggt ttattatttt agtgaacttg tcaagataaa
     2521 atttaaatag ggataacaaa attcgatata ggacaagccc ctctggcttg gcatatggag
     2581 aatgcgttag taaccactta aagcctaagg ttaaagtggt tactaaccgt taggttcaat
     2641 aaaaggaagg tgaaaattat gaaagattat tcaattaaaa atacaactaa ggaacaaaga
     2701 aaagagattg ttaattctgc acttggatta ggctcaattg attctcatga acctagtata
     2761 gttgttttag aattgttaga tagatatgtt gaaggagaat tggagataaa agaaattcaa
     2821 gatatagtgc ttaattctta taagagaaag tatcttgatg gtgatgaaga aggtattgaa
     2881 aaaatgatgg atgaaataat agaccaaaat atagatgctt taaaaaaatt atctgaatga
     2941 tatgaggtga actaatgtac attgttgcaa aaaaaattga caaagtaggt tgcattgcat
     3001 cagaggtaga aagtggagaa aggataggtg cattgattga atacttatca ttaacaatgc
     3061 ttgataaggg gattgaaatt gttgttttag ataagccaaa tatgtatgga gaatatgctc
     3121 catatgagtt tttgagcgag gttgagtttg tatgtagtgt tttatcaatg agccattagt
     3181 ggtctaaata gaccactagg gcgttccaaa agtgaccact tttggtatat aggtttttaa
     3241 tgcctcgcag agcttaccac tttgccaaca tagtgcaaag tatagtaagt tagacttata
     3301 tatataaata tgttgtttta aattacattg gtaatttaaa acttccagaa catggacagt
     3361 acatttgtaa gtacatccat gttctggaag tcatattttt atatataagt cttggttttg
     3421 ttatctattt gtttcagata ttcgtatgta aattctaata ccctttcatt agtaatgttt
     3481 tgaattatag aaatcatttc tttttttaag ttaatttcta ttgagcagcc ttgtaaatta
     3541 ataaattcta agttgttgtt ttttaaaaat tctatgaatt ttttaatgtc tgtgattttt
     3601 tcttcaccat aaaattcttc aatcacaatt aattgtttaa gtagtggatt tcttttatca
     3661 tccatattat cctccttttt gactataact caaaaaagat atatactgta cttgcgatgt
     3721 atagtgctat atgctccttt ggggctatag tgcttttttt attataataa caatgcaaat
     3781 acgatttgtc aatacagaaa gtaattattg aatgtattga catgaacggt attctgtgtt
     3841 attttataaa tataaaatta taaaaggaga aattaaatgt tattttcgaa tagagaaaaa
     3901 agagataaat atatacaaat acgtcttaca gaaacagaaa aacagaattt aaaagacata
     3961 attgatagat tgaatgaaga tagttctaaa aaaataagtt tgacagattt tatattaaat
     4021 gctattgatt actacataga gcatgaatgt aaaaattata aaaagtaaaa aagagggggt
     4081 attttttatg aagggtatta aaaaattaac atcattaggt ttagtcggat tattattttc
     4141 aatgagtgta tttcactcaa atgcttttac tcttgaaaga attcaaggaa aaaataaata
     4201 tgaaactgct ggtcttgttg cagataagca acaatatact actgctatat taattaatgc
     4261 agatagtaca atgtcagatg gtctttcagc aagtggactt gcaggggtta ataatgcacc
     4321 aattttatta acacaaaaaa ataatatacc aaaagaaact cttgaaaggg tagaaagagc
     4381 caaaaaagta tatataattg gtggagaaaa ttctatagat aaatcaacag aaggtatttt
     4441 gaaagataag ggaaaagaaa ttaaaagact tcaaggtgat gatagaataa aaacaagcta
     4501 tagtgttgcc aaagaaatta atgcaataaa aaaagttaat acgataatgc tgactaatgc
     4561 ttttaagggc gaagtagata caatgagcat agcaccaatt gcaataatga aaaatgcacc
     4621 aattatactt acaaatggaa gtacgatacc atttaaattt gataaaacta ttacaagtta
     4681 tgttgttgga gggaataaaa gtatgactga tgatttactt ataaagacta attcacaaag
     4741 actaactgga gttgataggt atgcaactaa tagaaacata ataaaatcat tttataatag
     4801 agcagtagaa gaattttata ttgcaagtgg aacagattta gtatatcctc ttattgcttc
     4861 aactatagtt aaagataaac caatggtttt agttaatgaa agtaataata aagaaatatt
     4921 aagaaatgca tctaaaatta ctgtagtagg agatttagat gaatctataa taaaaaaatg
     4981 tttagatgtt aaagatggaa agtcagaaac caaaactaaa aaagaaaaaa tctatgtaga
     5041 aggatttgga tgggttgagc caacagactc agaaggagaa gaaggtaact ctgatggaga
     5101 ttttaataaa attgtagcta atatgtagtt gtatactata cattaaaaga atataatagt
     5161 attaagagat ttattaggtg tcccaaaatg taataaatgt aataaaaacc aaataactct
     5221 atatttatgt tcattttgga atgtttgtta aatgtcccaa aatgtccgga atgtccgaaa
     5281 tgtccgaaat gtaataaaat tcaaataact ctatatttgt gttcatttta aggtatttgt
     5341 tgaatgtctg aaatgtctta aaaatgaaaa aacactaaag gtttgtaaat ttttagaaca
     5401 aatattttta taaaataaat aagtttaaat taagagagca gataatatct gctctctttt
     5461 ttaatacaaa aaattattta ggaggtaaaa atctatgaaa tttaaagaaa ttaaaaagaa
     5521 aaattatgtt attggaggtg ttctattaat tatgatttca gtttcaattt ttatttttaa
     5581 agattcttcg aataaaggag aatccttaac taatcaagtg aaaaatgaga caggtcataa
     5641 gataaaagaa gaagtaaaaa atgttgatgt tccaaaaatt aatatggatg aaggaagtag
     5701 tgaatcaaaa atgaaaatgg cagaaaaaat taataaagaa aataatataa cacaggaaga
     5761 ttacagaaat tttagagaag gtatagaaaa aaaagaaaat gaacctataa aaaaacccaa
     5821 taaacctaat aaacctaata aacctaactc taatattatt aacccagagg taaaaccaag
     5881 taataatgaa aattctgtta aaaataacaa tccagaagaa aataaaaata atgagagtga
     5941 aaataaatca gaagagaata aaaaagaaaa ggtctatgta gaaggatttg gatgggtaga
     6001 accatctgaa tcacaaggca aagaaggtac ttctaatgga gattttaata aagttgtagg
     6061 tcaaatggat taagaaagag agggtaagga ttatgaaaaa aagattatta attgtatttc
     6121 tttgtgtaac tttatttatc ccaatacttg tttttgcaga tggtagtggt aatgtagatg
     6181 gaggtggtgg aagcacaggg agtggtggag ataagaaaaa ttattggaca gctggtgatg
     6241 aaggcgtaag aattacaata attgataata aaactcaaaa acctgtgtca actccaatag
     6301 attttacaaa taggaatata aatgtaaaag tacattttgg aaaagtatct aaaatatcat
     6361 atagagaagg gaaaacatta aatcttgatt caggtaaata taattatatt aatcctaaaa
     6421 ataaattgcc tgtcataatt tctagtaaaa cttatggaaa atctgatata aaaaagatta
     6481 aagaatattt ttgtaatgat tggacacaga gaatcatagc aaaagaagca aattttaatt
     6541 atgaagaaat gcaaaatggc gagtataaat tattattaga gcctgttgca tatttcactt
     6601 ttaatggtaa caaaatggct atgactgcaa cagaagcagc gttatacaat aaaaagttag
     6661 gtggaaataa attaagaaat aaatttgcaa gtatttctca taaaaaccta cctttagcaa
     6721 tgtttttaga aaaagatgat ttaggtttca aggcttggaa aggtatctct gatacagata
     6781 aggcaaaggc aaaagatgat gatactataa taaagatgct agggttaggt attgtatatt
     6841 ttaataatga gcctacaaaa gatgatgaag ttacaatacc accagtagaa ccatcaacac
     6901 cagaaaaccc agataaacct tctatagatg gcaaagaata cgaatatcat acggatacag
     6961 atgttatatc ttctgtgaaa ataaaaggct ctaatcgctc tccagataac cctgtaacag
     7021 ttaaatttaa aataaaaaac caaacttaca atatgaataa tatatatttg ccagaggaag
     7081 gtggagaaca atatgcatgg gttaagtggc acacacctaa gacaccagaa aaaataatta
     7141 tagaagtaag ttcaaatgca accactgata aagcaaaaat tatagctgat gttgttgagt
     7201 tagaagaaaa aacccctcct gatccagttg caaaagataa aagaccaaat aattttaagg
     7261 taccaacttt gccaaagaaa gaagaaacta aaacattaga atggggtaaa tgggattgta
     7321 actggaaagc agtttgggtg tattatagtg atggagaagg tggtggagaa tgggtagacg
     7381 aaggttattg ggtttggaag tggataccat atagtgcaac tataaatgca actatggata
     7441 caatcccaag tgagcattgc atgacacaga aaaaaacaag agatgtatat gagatgaaat
     7501 cggcttatgg aatagaaact ttggtgaata gtaaaatcac ttttaatgct ccagaagata
     7561 gtgtaacagg ggggcaaaat gttattgtta cttttccaga gtttggctat gaaaaatata
     7621 atagagtttt acaaaacaca aagataggtt atgattctag ttttgagttt aagaaaaaca
     7681 aatattctac atataatgca aaaactcatt ttacaccact ttggtattta gatggcaaat
     7741 actctacata tgccaccgta atagattctt ggacacctgc aggtcaatta aaaacaaata
     7801 tttacaatga taaaattact ataaaaggga atgtatatga tgattggcat ataggtcctg
     7861 taaaataagt tgaagaaagg atttttttta tggcattttt cgtttatcat ttggcaaagt
     7921 atagtaaaaa taatattgtt ggtattcaaa aacataatca aagacaaaat aaatcttata
     7981 aaaacaaaga tattgatatg tcaaaaagta aactaaatat agactttgaa aataaagata
     8041 gaataaaata tttagaaaaa gtagaaaaaa ttataaaaga gaataggaca aaaggaaaag
     8101 ttataagaaa agacgcaatt ttgatgtgtg aaagtgtgat ttcagcaagt ccagaatttt
     8161 tcctaggtat ggagcaatca gaaaaggaaa aatattttaa atactcatat gaatttttaa
     8221 aagatagata tggtgaaaaa aatgtaattt ctgctacagt acactttgat gaaacaacac
     8281 cacacatgca ttttagtttt gttccaataa catcagatgg gaaactaagt gctagagatt
     8341 taataacaag aattgaatta aaaaaaatac aggatgaatt accaatatac ttacaatcaa
     8401 aggggtttga cataaaaaga ggacgtgagc atagcgaagc aaggcacatg caaccagaag
     8461 attataaaaa acaacaaatt caagaactta gggaattaga aaataaatta gaatatgtaa
     8521 aaaaactatt taaaggcttt gagaagcctt taaatagttt taatgaacta gaacctgcaa
     8581 atatcaaaaa aaaccttata acaggcaaag aaacggtata taagaatgat tatgacgagg
     8641 taatagatat actaaaggga acaatgaaag aaaatctaga acttaaagaa aaattacata
     8701 atgagcagtt gaaaacaaga aatttagaag ttaagttaaa tacagtaaat gaatatgaaa
     8761 gattgactca tgaaaaagaa aaaaaattaa aattggaaaa agaagaattt gaagaacaaa
     8821 aacaactaat atctgaacgt gaaaaaaata ttgagaaagt actagaaaat aaagataaaa
     8881 ataataaaaa gattcttgat gaaaaggaag tagaaataaa tgagctagaa aaacaatata
     8941 aaaatttatc aagaaaatac aataacacaa taagagaata tgaaaaagtg attgaagaat
     9001 ttagtaataa tgatatatat attattttta ataagtctag taccaaagag atacaagaaa
     9061 atgacacata taagtttaaa gatgccaata atttcattaa agaattaaat aaaatgtgtt
     9121 tcactaataa tgaaacaaga gaaatagggt ttcaaatgtc atttggaatt aataatggtg
     9181 tttttatgga aaaaaatatt attcttggac aatatagaga tatggatttg aaacgtgaga
     9241 ttattagtat aaacaaatct aataataaag tactttctaa agatttattt agatattagt
     9301 aataatttag gcttagaata tataattatg caacttctaa gcctagcgaa atttaactcc
     9361 ttaaactatt tcgttgttta taaagaatta gcccaactgt aagaatagcc aataataata
     9421 tggtttttat aatttcagca tatatcgtag tgttgaatgc tataccatta atagtaaaca
     9481 tatactttct attaatcatt gtatagatac atatgagtaa aaataaaata tttttttatg
     9541 ctttgaaaga aagtattttc ttaaaaaaaa tatttcaagt attataaata ataaaaataa
     9601 acctatatcg tttacaccta ttttaattcc cacaatatca cttccccccc gtaatatatt
     9661 tatacttact ttagagttta tcaaatattt ctgaaaattt atatatacat attttcaaat
     9721 taaactagtt ctaaaattga cgtttagaaa ccatgagatg agatgtagca attctaatat
     9781 tttaataatt tatattttat aactattttt ttagttatgc taaacgaagg cgttaaacta
     9841 aatttattta aggtatattg ataacaactt ttttcttctg tagtattaca taaaaaaata
     9901 ttataattta ttatttttat tagttctttg ttatgaaata aatagcttat taaacaaaat
     9961 ctaaaatcac ttatattgac taatcatctt tgtttatttt tatacattaa tctggtcacc
    10021 caaaaaataa aattaaatat tctaagccat catcattcta aaataaaaaa gaactagcta
    10081 taactctagt tcttatctga cctattactt cttttaattt ctccaccctc gaatattttt
    10141 atccactcat acaatgtagt tctgccaata tttaatatct ttgccacttc attcttcttt
    10201 aagtttccat caaggatttt attgtaatat tttataaatt ctttgttagt ttctaaaagt
    10261 tccttagtta ctttttgtct accaaaccat ttgcctgttt ttgtatttat ctcccctttt
    10321 ttcttatcct cttttaactg gtctattttc tgttgagttc tctcttgtat tgtttctctt
    10381 tccatttctg caactgcccc aaatatacca agtaataact tataagtatt gtttcctgtg
    10441 tctatacctt cttttaatat ttttaccatt acaccttttt gaactaatgt atctataata
    10501 tcaattaagt ccttcaagtt cctacctaga cgacttatag actcacagta aacaacatct
    10561 ccctcattta cttctaataa cattctattt aatgcttttc tttcttttgt agctcctgta
    10621 attttatccc tatatatttt atcaaactta attccctcgt tttctaaaac tctttcttgc
    10681 ctactattat cctgttttct tgtgcttact ctttcatata ctattatcat aactataccc
    10741 ccgtttattt tgttataata attatacaat atattttttg tccgtttaaa ctatattttt
    10801 tgcattttaa ataccgaaca tatcttgata cttattgcca acacttttag tttaaatcta
    10861 tataaaatat tacagtacgg ttaaacgttg gtttattcaa acataattat gcaataatat
    10921 gactatttat ataatttaat atgtttttga agcacttatt aacgtatctc tttcagatgt
    10981 agatccctta tatgctataa ctacagatga aacacgatat gttcctttac ttatagattt
    11041 tgttttactt aacgtacaaa gtttatcatt ttttgaaacc gtccaactat caacagtttg
    11101 ccagttatta ttaacttttt tctgaagtgt agatgttata gttgtttttg taattccaag
    11161 ctttcctaga agtgtagttt ttataatcgc attacttcca gatatactaa gctcactagt
    11221 agctttactt atattagtat atctgggctc ttgaattgtc ataatatttt cttgtaaact
    11281 atctgcaaaa actggtaaag atatagataa attagtgaaa ataaatgata atgttgttat
    11341 aaatataaaa ctttttttta attttaattt catagaaatt acctcctaat aatatttata
    11401 tatataacga tataaaaagt aattttgtga catttttatt ttatattttc tgctattttt
    11461 attatttcat ttttagtatc ttgtaattta atgtattcaa tataactcaa tgtaatataa
    11521 tatgaatatg tattatcatt ccatattaaa gaaaagtcat tattttttat gatatattct
    11581 gcatttattc cattaatctt tacattttcg ataatagctt cttctgtatc tattttcata
    11641 gttgtgtcag caattttaga gtaattaaaa actatttcat ctccatcgtc attactaaaa
    11701 aatacgaatg tctcatttga aatattagtt ttgttaattt ctttaaaacc ttttggtaaa
    11761 tatgaaagat ttaattcatt aaattttcca atatcaatat tttcattaat tgagaatttg
    11821 tatgtggtaa actttgtata tacttctttt attacattga aaaattgttc tctcatagca
    11881 tcaacactca ttgtcattgc aaataaacca attataatta ttaataatgc agctactata
    11941 ttttttgaat agctataggt attttttctt aaagtttttt ttgtttcttg ttttattaat
    12001 ttttccattt tattttcaaa actttttgaa aaagtatagt ctatatcatc ttcttttgga
    12061 actttactaa ttagaatcaa ttcaacattt tcaagtgctt tacgaagttc ttcatctgtt
    12121 attttaaaat cattcaatat taaacaccca catccttaat aaaaatatat tttagctctt
    12181 ctctagctct agatagtctt tttctaacat tttcaggttt tatatctaat atttgactaa
    12241 tttctttatc atttaattca tgttgtaatt ttaagagcaa tacatctcta tgcttttcag
    12301 aaagcttcat tatagcttct tctaaataac ctattgaata ttcatcaatg tgaaatttat
    12361 cagattgaaa ttctttttca agggatttta ttttttcatt ttctcttttt cgttttcgat
    12421 agatatcttt ggaagtattt tttactatta tgataacaaa agctttggtt ttataacttt
    12481 tgatatcgtt tattttattc aaatttttaa ttatctttaa aaaactttca tgaacagcat
    12541 cttctgccaa acattcatct tttaaaattt cattcgctat ataaaacata gtttttttat
    12601 atgtattata taataattca aatttggaga tatcattttc agattctaat aaatttaaat
    12661 ataatatcat gtataaattt tccccttaat catatatata ttttccttat acattatact
    12721 cttttaaata aaaaatttaa aaaaattgtc acaagattaa tttatataac gttttatatt
    12781 atgaaaacaa taataaaggg ggaaaaacta tgtttttaaa aaaattaaaa aaatctacag
    12841 ttattgcttt aacaggacta atgctaataa gttcaacagg aaatctggct tttgcattag
    12901 ataataaaaa tgaaggggct aaaaatgtat ctgaaaattc aatagtagaa ccaagggttg
    12961 ttagtaattc aaaaactttt tctgttggag gaaagagtta tacaaactat acatcagcta
    13021 atattaccct ttcaagtggt gcctatggtt caactactat aaaatctaat tctggagcag
    13081 ttggagaaaa tagattatat gcacatgcca atttatataa caaaaatgga caagttgtta
    13141 aaaccgctga ttggaaatct aatggtagtt ctacttctac acttacaaat acaacaggaa
    13201 atagcttttc aaaaggtaca ttttatacaa aaggaactac taaagtatat aatggaaatg
    13261 gatatactac acaatctgca aatcaaagtc ctaatgtagt ggttaataat ctaggtacta
    13321 gaatttcaaa agaagagtta aaagaacgta tggatttata tgaaattaaa aatatgctac
    13381 cagcagtagg gattgataat ataagtggct atatttcaat ggatgaccta tatgatgaac
    13441 ctaattttcc tgaaactcca gaagaagcta ttacatatca aaagataaga gctaaagcta
    13501 gttatagaat gattccactt tatgataatg atggtaaaac aataatagga gagtatcgta
    13561 ttgattcagc taattaatgt aatgttacaa aattttatta atttataaat taaaaggcta
    13621 tgaattatag aaataaaatt ctattttcat agctttttat aaaattatat aagttaataa
    13681 atatagagtt aattaaaatt attttactta aactgaaact atatatgttt ctaattaatc
    13741 taattaatat agaatacagt aaaggagatc tacatgagaa aattatcatc atttaaaaat
    13801 ttaataaatc aaaatcaaaa tgataatgat aatatggtaa taagtgccat aaatggtaat
    13861 aagcatgcaa ttgaacaact tataaatgaa caaaaagagt atctatataa aacagcattt
    13921 ctatatacaa aagatgaaaa tgaagcaatg gatatatgtc aagagaccat ttataaaaca
    13981 attctaaata tacataagtt gcgggaacca agttgtttta aaacatggat gacaaaaata
    14041 cttattaata atgttaatga ggtaaataga aataaaatca aaatatttga taatgatttt
    14101 caaattgaat taattaaaga tatatcatat gaaaaattag aagaaaagtt agacttatat
    14161 agtgcaattg atctattaga agaaaaattt aaaacaccta taatctttca atattttcaa
    14221 gatatgacta taaaagaaat agcagaggta cttgagtgta atgaaaatac agtcaaaaca
    14281 tacttaagaa gaggtaaaaa aaaattatac gaaatattaa tgagggggga gatagatgaa
    14341 aaagttagat aatataaaaa tgcccgataa ttttgatctt gctatacaaa catctataag
    14401 caaagctttg aatg
//
LOCUS       DAMJVK010000098        14205 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.102, whole genome shotgun sequence.
ACCESSION   DAMJVK010000098 DAMJVK010000000
VERSION     DAMJVK010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14205
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.102"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            431..1324
                     /locus_tag="PC414_003048"
     CDS             431..1324
                     /locus_tag="PC414_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HDF2465428.1"
                     /translation="MSNKIVSKRQLTDSIYLMEIEAPRVAKSSQPGQFIIIKNDEKGE
                     RIPLTIADYDREKGTVTIVFQTVGASTKKLAMFEENDFVMDFVGPLGQASEFIHEDIE
                     ELRNKKILFVAGGVGSAPVYPQVKWFKEHGLDVDVIIGARTKELIILEDDMKKVAKNV
                     YVSTDDGTYGFNGRVTDLLKDLVDNQGKKYDQAIVIGPMIMMKFMCQLTKELNIPTIV
                     SLNTIMIDGTGMCGGCRVSVGNETKFACVDGPEFDGHLVDFDQAMRRQSMYKTQEGRA
                     MLKLEEGDSHHHSNCGCGGNK"
     gene            1324..2718
                     /gene="gltA"
                     /locus_tag="PC414_003049"
     CDS             1324..2718
                     /gene="gltA"
                     /locus_tag="PC414_003049"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HDF2465429.1"
                     /translation="MDAKKVKVPVREQEPAVRATNFDEVCLGYNKEEAMAEANRCLAC
                     KKPKCVGGCPVGIDIPGFITKIKEDDIEGAAKVIAKSSSLPAVCGRVCPQESQCEGVC
                     ILGIKSDAVSIGKLERFVADWSKENDINLSDTEPKKNQKVAVIGSGPAGLACAGDLAK
                     KGYDVTIFEAMHEPGGVLTYGIPEFRLPKQAVVQPEIDNIRKLGVKIETNVIVGKTIT
                     VDELIEDEGFEAIFIGSGAGLPMFMNIPGENANGVFSANEFLTRVNLMKAYRDDYDTP
                     ISSGKKVAVVGGGNVAMDAARTALRLGSESYIVYRRSEKELPARAEEVHHAKEEGIIF
                     NTLTNPKEILVDENGYVKGMVCIRMELGEPDDSGRRRPIEIEGSEFVLDVDTVIMSLG
                     TSPNPLISSTTKSLDINKKRCLITDENGQTSKEGVFAGGDAVTGAATVISAMGAGKTA
                     AASIDEYLKAKVNA"
     gene            3084..5303
                     /locus_tag="PC414_003050"
     CDS             3084..5303
                     /locus_tag="PC414_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2465430.1"
                     /translation="MKKREKVFDGLVFKHILILLILLVLIDLTSTIIYASNSMEITSR
                     NMIEASKRELENYLEVNISLLKALSQDDRFSNGETSLIEKGKLLSPYQKEYNLFMIGI
                     TDTKGNASSTYRENVNSIKDKPSFEKAIKTKQVVVSDIEVSNVTGDKVFIIYVPIVKN
                     NEMIGTIFASFYFQDVNNLISRSNFDDSIKFLMIDKDYTIISHPNKKYVNDKSKMLDL
                     EGNIIGTTKSEILKNINKKCQGDFLSWDNWRLYNVKYTNIKWTNWTLVSKCNIFKNFK
                     NLIVNFMIKLYFYIVIFMILWKLSNAKLIEQLKKLAYYDSLSGIKNKEKFRKDSMYIL
                     KNYYQDNFYLVQLDVNKFKYINEMFGYAEGNKILIHISQVLNNNTNKYEICARMDNDH
                     FILLIACNTEDELLNRLSKINKEICNLNTTNSSKYKIVMSSGIYKITKKDDIKKIDLL
                     IDRANIAAKSKKEKYEHSYSFFNEDTRNRLYKEKRLEDNMNKALEKGEFIVYYQPKYS
                     LDDVNEIEGAEALIRWNSPEFGFISPIDFVPLFEKNGFIVNIDMFVFEEVCKTLNKWI
                     NKGYTPVPISVNMSRVHLYRDNFIENITDLISKYNISPEFIELELTESVVFDNLNILI
                     DIMKKIKEIGFLISMDDFGSGYSSLNLLKDLSFDILKLDRGFLIETTDTKRGKIIISK
                     IVEMAKAIDIKVICEGVETYEQVEFLKEIGCDKVQGYLFAKPMVLDEFEKHLNFKFD"
     gene            5450..6196
                     /locus_tag="PC414_003051"
     CDS             5450..6196
                     /locus_tag="PC414_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2465431.1"
                     /translation="MNILSIRNISKTYCGNIPFKALDKVSLNIEKGEFVSVMGPSGSG
                     KSTLLNIISTVDRQSEGEVVLDGYDVSKLKGEKLAEFRRKQLGFVFQDFNLIDTLTVG
                     ENIMLPLTLEGASIKDMNIQTKSISKFLGIDKILDRKTYEISGGQAQRCAIARAIINK
                     PAILLADEPTGNLDSKSTDDVLKLFTRINKEQKVTTLMVTHEAYSASYSDRVIFIKDG
                     CIYTEIKKSESSNSFYSDILAVLSQIGGVR"
     gene            6196..8076
                     /locus_tag="PC414_003052"
     CDS             6196..8076
                     /locus_tag="PC414_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2465432.1"
                     /translation="MDFRKFAYNNIVKNFRAYLAYFLSSVFSIMIFFVFAVSMFHPII
                     TKSGMQSGSTAFMALMTSELIIFGFSLLFILYSLGNFLKYRLKDFGVLMIIGMSNRQL
                     KKLILIENLIIGFLAVMLGILLGLSISKLFLLYLSKLFYMNLTEFYFPVKAMLLTIVS
                     FMILFLITGPMSLKLLSKKSILELLVGTKKPKKEIKSSKLFGIIGVICLILGYAMILF
                     GNKLKINGGMYTTALLITLGIFLFFSQFTILILKYLKNKEKFYKNKVNMLLISNLIYK
                     LKDNTRLLFLITILLSATLVAFTTTSTLVSSQGEASKNNFPMVYSYFSEHNNEKEYEE
                     LQQIREAIKGYDYKELSFPVLKYNREVLLSVKNYNKLSKELGLDKISSKLEKYEGVII
                     PSSNSQEHINSLKQLKNYSIKNLNIEVKKSKNKIIFPTGLFSKVVVISDDLYNEVKGE
                     FSKINFHGFDYKNWQSSAQITENLKEKHFNLDREYNRSYFLTLPDMYLVELQQSKILQ
                     FMGVFIGVILFIAVFSFLYFRLYTDEIEDNVKYKKLSKIGLSFKNMNKIVSIEIGTLF
                     FIPYFIAIINSIISLLFLNQVLNNSFKLENLGILLLISSIYSLYFYLLKKIYTRKVWF
                     FD"
     gene            8195..9586
                     /locus_tag="PC414_003053"
     CDS             8195..9586
                     /locus_tag="PC414_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2465433.1"
                     /translation="MENQQLLGTERISKLLLKYSIPAIIGMLVNSLYNVVDRIFIGNI
                     PGVGPLAITGLGVTMPIMTIILAFGMLIGIGTTTTISIKLGQGKVEEARKLIGNAMTL
                     SVITGIIIMILGILFANKILTLFGASENTLIYAKSYINIILLGTVVNLLSFSLNHSIR
                     ADGSPKISAGIMIVGCLTNIVLDWILIFGFNLGIQGAAIATVTSQALTAILTIGYYIS
                     GKSNLRFSKSNLKLDKKLIKAVFAIGMSPFAMQLAASLVQVISNIALKTHGGDLAIGA
                     MATISSIAMVFLMPIFGINQGAQPIIGFNYGAEKYDRVKKAYLGSLVVATIILCMGMV
                     VVMLFPEAIIGIFNKDPELMNISVNGLRIYLLMLPIVGLSVTGTNFIQSIGKAKMAML
                     LSLLRQVILLIPAVLILPTFLGLQGVWTAQPVSDFIATVITGIVVFRELKRYTPKTEK
                     LNENERLNEITTE"
     gene            9693..10733
                     /locus_tag="PC414_003054"
     CDS             9693..10733
                     /locus_tag="PC414_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDF2465434.1"
                     /translation="MEFTKKSKLKNMYVNMNVARMLSKINEYKGRQLLYKKQPKEILE
                     NLEKKSLVDCSESTYIGNQRDSSNFNLEKLISNEVTPRSREELSIVEYRDVVKTINSA
                     YESIPISSQTILELHGYLYKFSSTRGGSYKSDNDFIEHNLKPSEFISIDSSVNKVEKA
                     VEEICEAYNTLIEEDEIDILILISAFVLDFILIHPFKEGNIKMARVLILLLLNKNGYE
                     VGRYISLGKIFDDSSYEYYSNLNYLKASIGSEKADMNAWIEYFLETILTAYEKLDDSL
                     NISDKKRQTKTSRIEKIINSTLGYFTKEDIRDLCPDIPEPTINRVFNNLRKQDKIEVV
                     ARGRSAKWKKKY"
     gene            11222..11875
                     /locus_tag="PC414_003055"
     CDS             11222..11875
                     /locus_tag="PC414_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDF2465435.1"
                     /translation="MKKKLLYINVNSKPEDLSSSKTVARKFINKFMEKNKDFEVEEID
                     LYKEHIPRLEYQYFEKRNSIVSEENAKNLDDKDRKELTKIRNLCDQFVSASVYVIAAP
                     MWSLSFPAPLKEYIDCIIQDGKTISFEGNDKPQGILNDIDRSMVYIQSSGGHIPWVLK
                     PVMNKGLNYVESIMKFIGIKKFDELLVDGTGTSEEERQAAIEKAAEKIDGIIDGMKF"
     gene            11951..12160
                     /locus_tag="PC414_003056"
     CDS             11951..12160
                     /locus_tag="PC414_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465436.1"
                     /translation="MREEKSNEKYDCYWCNQENNFCVEIKDNIVMIDDGTGTLKQAVF
                     IGYKQIQINLNCSHCQNLNRIKLNL"
     gene            complement(12300..12866)
                     /locus_tag="PC414_003057"
     CDS             complement(12300..12866)
                     /locus_tag="PC414_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HDF2465437.1"
                     /translation="MMNNELNTIILETLNNADITSNDIPSIDLYMDQIISLIDNKLSA
                     NKRFESDKILTKTMINNYSKEGLIKPVKGKKYTKEQILQMIIIYSMKNTLTIQEIKRI
                     LHGVYEKDNFSEKDLVSCYEKFMLIKENQRKNIPDFIESNFENISINPENKDDLLIAL
                     LSLTSMADQLKNISEKLVDRYFPDITKK"
     gene            13027..13680
                     /locus_tag="PC414_003058"
     CDS             13027..13680
                     /locus_tag="PC414_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HDF2465438.1"
                     /translation="MYQFFLKGRDPISSLTHFIGACLSLLATIILVFQSVTLQETSLL
                     MIVSVSVFGFSLIALYSASSYYHFLKGTPEQELFFRKVDHAMIYVLIAGSYTPICLNF
                     MEKKEGIIFVTAIWIVAFIGIIIKIFWMDAPRWLSTSIYLLMGWAIVFDINAFNSIPK
                     DCLRLLIMEGVSYSIGAIIYIIKKPNISPEFGFHEIFHIFIMIGSLFHFLAVLLYVL"
     gene            complement(13838..14014)
                     /locus_tag="PC414_003059"
     CDS             complement(13838..14014)
                     /locus_tag="PC414_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465439.1"
                     /translation="MYKYDDYVNLTNDLDLPVEIRNKCVRIVSVSENLEDIIIVHKFK
                     LYVVNEKYIKGIME"
BASE COUNT         5558 a         1402 c         2387 g         4858 t
ORIGIN      
        1 aatatgaaag ctggtattgc aatagattaa agccttatac acgtttagtt tttaaagaaa
       61 ttagtgttat tatgaaaacc taagttaatt tattaaataa ataattgaat aaaaaaattt
      121 tattatgtat ttataaaatt tttgttacaa atttggcata atatagtata ctttaagtgt
      181 gtattaggtt aaatctataa caaattagac gtatgatata gacttagtgt ttattggcat
      241 ttacaagaaa tttgttaata tcaactattt gtagtttggg cagttataat tattataggg
      301 gggatgtcca atacaaaaca atcaaccaag aactaatatt atattataaa gagttatagt
      361 tttgtgtgtt tatgttttta attaaattta aagtgggaaa aatatatttg ttagaatgga
      421 gaatagcgag atgagtaata aaatagttag taagagacaa ttaacagata gtatttattt
      481 aatggaaata gaggcaccta gagttgcaaa atcttctcaa ccaggtcaat ttataataat
      541 taaaaatgat gaaaaaggag agaggattcc tcttacaata gctgactatg atagagaaaa
      601 aggaacagta actattgtat tccaaacagt tggtgcttca actaaaaagt tagcaatgtt
      661 tgaagaaaac gattttgtaa tggattttgt agggccatta ggacaagcaa gtgaatttat
      721 acatgaagat atagaagaat taagaaataa aaagatatta tttgtagctg gtggagttgg
      781 ttctgctcca gtttatccac aagttaaatg gtttaaagag catggattag atgtagatgt
      841 aataattggt gcaagaacta aagaattaat tatattagaa gatgatatga aaaaagtagc
      901 taaaaatgta tatgtatcaa cagatgatgg tacatatgga tttaatggaa gagttacaga
      961 cctattaaaa gatttagttg ataatcaagg taaaaaatac gaccaagcaa tagtaattgg
     1021 accaatgata atgatgaaat ttatgtgcca attaactaaa gaactaaata tacctactat
     1081 agtaagttta aatacaataa tgatagatgg aacaggtatg tgtggtggtt gtagagttag
     1141 tgttggaaat gaaactaagt ttgcatgtgt agatggtcct gaatttgatg gtcatttagt
     1201 tgattttgat caagcaatga gaagacagtc tatgtacaag actcaagagg gaagagctat
     1261 gctaaaactt gaagaaggcg atagtcatca tcatagtaat tgtggatgtg gaggtaacaa
     1321 ataatggatg ctaaaaaagt aaaagtacca gttagagaac aagaacctgc tgtgagagca
     1381 acaaattttg atgaagtatg cttagggtat aataaagaag aagctatggc agaagctaat
     1441 agatgtttag cttgtaaaaa accaaaatgt gtgggtggat gtccagtagg aatcgacata
     1501 ccagggttta taacaaaaat taaagaagat gatatagaag gtgctgctaa agtaatagct
     1561 aagagcagtt cattaccagc tgtttgtggt agagtatgcc cacaagaaag tcaatgtgaa
     1621 ggtgtatgta tacttggaat aaaatcagat gctgtatcaa taggtaaatt agaaagattt
     1681 gtagcagatt ggtcaaaaga aaatgacata aatttatctg atacagagcc taagaaaaat
     1741 caaaaagtag cagtaatagg aagtggtcca gcgggtctag cttgtgctgg tgatttagct
     1801 aaaaaaggct atgatgtaac aatctttgaa gcaatgcatg agccaggtgg agttttaact
     1861 tacggaatac ctgagtttag acttccaaaa caagctgttg ttcaacctga aatagacaat
     1921 ataagaaaat taggtgtaaa aatagaaact aatgtaattg ttggtaaaac aattacagta
     1981 gatgaactta tagaagatga aggatttgaa gctatattca taggttcagg agctggtctt
     2041 ccaatgttta tgaatatacc aggggaaaat gcaaatggag tattttctgc aaatgagttt
     2101 ttaactagag taaacttaat gaaggcttat agagatgatt atgatacacc tattagttct
     2161 ggtaaaaaag ttgctgtagt aggtggtgga aatgttgcta tggacgctgc tagaactgca
     2221 ttaagattag gttcagaatc atatatagtt tatagaagaa gtgaaaaaga gcttccagca
     2281 agagctgaag aagttcatca tgcaaaagaa gaaggcatta tatttaatac attaacaaat
     2341 cctaaggaaa ttttagtaga tgaaaatgga tatgttaaag gtatggtttg tataagaatg
     2401 gaattaggag agccagatga ttctggaaga agaagaccta tagaaataga aggttctgaa
     2461 tttgtacttg atgtagacac agttataatg tcacttggaa caagcccaaa tccactaata
     2521 tcttctacaa caaagagtct tgatataaat aagaagagat gcttaataac agatgaaaat
     2581 ggtcagactt ctaaagaggg tgtatttgca ggtggagatg ctgttactgg agcagcaaca
     2641 gtaatatctg caatgggtgc tggtaaaaca gcagcagcat caatagatga atatttaaaa
     2701 gcaaaagtta atgcttaatt tgtaaattaa agagagggtg tctcaaaatg aactttttag
     2761 ttcatgaggc actctttttg tacaaaatca aatatgtttt aatcatttca acaatgaaaa
     2821 tttcattcct gtagaaagtc taacttttag gtcttagtac aatctctatt ttgatgtagc
     2881 cactttttta gatggtttaa taatttatca gttattattg taatatttga tactaagtaa
     2941 cataactttg tctaactatg ataaaatagt tatacaataa aggtcttgta aggagtgaat
     3001 aatgtgtggc taataaaatc agatttgtca aatctaacat taaataatag aaaaataaat
     3061 tgtgtaatta atggtatttt aatatgaaaa aaagagaaaa agtatttgat gggctagttt
     3121 ttaagcatat attaatctta ttaatactat tagttttgat tgatttaaca agtaccatca
     3181 tatatgcaag taactctatg gaaattactt ctagaaatat gattgaggca tcaaaaagag
     3241 aacttgaaaa ctatttagaa gtaaacatat ctttattaaa agctcttagt caagatgata
     3301 gattttctaa tggtgaaaca tcactaattg aaaagggaaa attattaagt ccatatcaaa
     3361 aggaatataa tctttttatg attggtataa cagatacaaa aggaaatgct tcaagtacat
     3421 atagggaaaa tgttaactca ataaaagata agcctagttt tgagaaagca attaaaacaa
     3481 agcaagtagt agtttcagat attgaagtca gtaatgtaac aggtgacaaa gtatttatca
     3541 tatatgtacc aattgtaaaa aataatgaga tgattgggac catttttgct tctttttatt
     3601 ttcaagatgt aaataattta ataagtagaa gcaattttga tgattctatt aaatttctaa
     3661 tgatagataa agattatact attatttctc atccaaacaa aaaatatgta aatgataaaa
     3721 gtaagatgct tgatttagaa gggaatatta taggaaccac taaaagtgaa atcttaaaaa
     3781 atataaataa aaaatgtcaa ggtgattttt tatcttggga taattggaga ttatataatg
     3841 taaaatatac gaacataaag tggactaatt ggactttagt atcaaaatgt aatatattta
     3901 agaactttaa aaatctaata gttaatttta tgataaagtt gtatttttat atagtaatat
     3961 ttatgatatt gtggaaatta agtaatgcta aattaataga acaacttaaa aaattggctt
     4021 attatgattc tttaagtgga ataaaaaata aagaaaagtt tagaaaagat tctatgtata
     4081 ttttaaagaa ttattatcaa gataattttt acttagtaca attggatgtc aataaattta
     4141 aatatataaa tgagatgttt ggatatgctg aagggaataa gatattaatt catatatcac
     4201 aggttttaaa taataataca aataaatatg aaatttgtgc taggatggat aatgaccatt
     4261 ttatattgtt gattgcttgt aacactgaag atgaactttt aaatagatta tctaaaataa
     4321 ataaagaaat ttgtaactta aatactacta actcatctaa gtataaaatt gttatgtcaa
     4381 gtggaatata taagattact aaaaaggatg atattaaaaa gattgacttg ttaattgata
     4441 gagcaaatat tgcagcaaaa agtaaaaagg aaaaatatga acattcatat tcatttttta
     4501 atgaagatac aagaaatcga ttgtacaagg aaaaacgatt ggaagataat atgaataaag
     4561 ctttagaaaa aggtgaattt atagtatatt atcaacctaa gtatagttta gatgatgtaa
     4621 atgaaattga aggagcagaa gccttgataa gatggaatag tccagaattt gggtttattt
     4681 cacctataga ttttgtacct ttatttgaaa agaatggttt tattgtaaat attgatatgt
     4741 tcgtgtttga agaagtatgc aaaacattaa acaagtggat aaataaaggg tatactcctg
     4801 taccaatctc tgtaaatatg tctagagttc atttatatag ggataatttt atagaaaata
     4861 taactgattt gataagtaaa tacaatatat ctcctgagtt tattgaactt gaactaacag
     4921 aaagtgttgt gtttgataat ttaaatatat tgattgatat aatgaaaaag attaaagaga
     4981 taggattcct tatatcaatg gatgattttg gttcgggata ttcatcttta aatctgttaa
     5041 aagacctttc ttttgatatt ttaaaattag atagaggatt tttaatcgaa acaacagata
     5101 caaaacgtgg aaaaattatt atttctaaga ttgtggaaat ggcaaaagcg attgatatta
     5161 aagtaatctg tgagggcgta gaaacttatg aacaagtaga gttcttgaaa gagataggtt
     5221 gtgataaggt tcaaggatac ttatttgcta aacctatggt attagatgaa tttgaaaaac
     5281 acttaaactt taaatttgat taatcattaa taattattag actataatat accctaacaa
     5341 cttacttaca aatatgtaag ttaattgtta gggtattttt atggttaaag tagtatattc
     5401 acgatattat ggtattgtag taaaaataaa aaattatgga gaatataaaa tgaacatatt
     5461 gagtattaga aatatatcaa aaacatattg cggaaatatt ccatttaaag cattagataa
     5521 ggttagtcta aatatagaaa agggagaatt tgtgtctgtt atgggaccat ctggaagtgg
     5581 taaatctact cttttaaata taatatctac agttgataga cagagtgaag gtgaggttgt
     5641 attagatgga tatgatgtaa gtaaattgaa aggagaaaaa cttgctgaat ttagaaggaa
     5701 acaacttggt tttgtatttc aggattttaa tttgattgac actttaactg ttggcgaaaa
     5761 tattatgttg ccacttacac tagagggggc aagtatcaaa gatatgaata tacaaactaa
     5821 aagtatatct aaatttttag gaatagataa aattttagat agaaaaacct atgaaatttc
     5881 aggaggtcaa gctcaaaggt gtgctattgc tagagctatt ataaataaac ctgccatact
     5941 tcttgcagat gagccaactg gaaatcttga ttcaaaatct actgatgatg ttttgaaatt
     6001 atttactagg ataaacaaag aacaaaaggt aacaacactt atggtaactc atgaagcata
     6061 tagtgcaagt tattcagata gagtaatttt tataaaagat ggttgtatat atacagaaat
     6121 aaaaaaatct gaaagcagta attctttcta ttctgatata ctagctgttt tgtctcaaat
     6181 aggaggtgta agatagtgga ttttagaaag tttgcttata ataatatagt taaaaatttc
     6241 agggcatatt tagcatattt tttaagttct gttttttcta taatgatatt ttttgttttt
     6301 gcagtgtcta tgttccatcc aattataaca aaatctggaa tgcagagtgg ttctactgca
     6361 tttatggctc ttatgacttc agaattaata atattcggtt ttagtctact ttttatattg
     6421 tactctttag gaaatttctt gaaatataga ttgaaagatt ttggagtatt gatgataatt
     6481 ggaatgagta acagacaact gaaaaaattg atattaattg aaaatttgat aataggattt
     6541 ttagcagtga tgttaggaat attacttggt ttatcaattt caaaattgtt tttattgtat
     6601 ttaagcaaac tgttttatat gaatttaact gaattttatt ttccagtaaa agcaatgcta
     6661 ttgactattg tatcatttat gattttattt ttgataacag gaccaatgag cttaaaatta
     6721 ttgagtaaaa agagcatttt agagctttta gtaggtacaa aaaagcctaa aaaagagata
     6781 aaatcttcaa agctatttgg aataattggt gtaatatgtc ttatattagg atatgctatg
     6841 atactatttg gcaataagtt aaaaataaat ggaggtatgt atacaacagc actattaatt
     6901 actttgggga tatttttatt tttttctcaa tttactattt tgatattaaa gtatttaaaa
     6961 aataaagaaa aattttataa gaacaaagta aatatgttac ttataagtaa tttgatatat
     7021 aaattaaagg ataatacacg attgttattt ttaataacaa tacttttatc ggctacttta
     7081 gtagcattta caacaacttc aacacttgta agttctcaag gagaagcttc taaaaataat
     7141 tttccaatgg tttactcata tttttcagag cataataatg aaaaagaata tgaagaacta
     7201 caacaaatta gagaagcaat caaaggatat gattataagg agttaagttt tcctgtactt
     7261 aaatataata gggaagtctt actaagtgta aagaattata acaaattatc aaaggaattg
     7321 ggattagata aaattagttc aaagttagaa aaatatgaag gggttataat tcctagtagc
     7381 aattcacaag aacatataaa ttctttaaaa caattaaaaa attatagtat aaaaaattta
     7441 aatatagaag taaaaaaatc aaaaaataaa ataatttttc ctacaggact gtttagtaaa
     7501 gttgtagtta taagtgatga cttatacaat gaagtaaaag gtgaattttc aaaaattaat
     7561 tttcatggat ttgattataa aaactggcaa agcagtgcac agataactga aaatttaaaa
     7621 gaaaaacatt ttaatttgga tagagaatat aacaggtcat attttttaac attaccagat
     7681 atgtatttag tagagttaca acaaagtaaa attcttcaat ttatgggtgt ttttatagga
     7741 gtgatattat ttatagcagt ttttagtttt ttatacttta gattatatac agacgagata
     7801 gaagataatg taaagtacaa aaaactatca aaaataggtc tatcttttaa aaatatgaat
     7861 aagattgtta gtatagagat aggtacattg ttttttatac cgtattttat agcaattata
     7921 aatagcataa tttctttact gtttttaaat caagtattaa ataatagttt taagttagaa
     7981 aatttgggta tattactact gatatcatct atatattcac tttattttta cttattaaaa
     8041 aaaatttata ctagaaaagt ttggtttttt gattaaagtg gtatataata gatatataaa
     8101 ttaatattta aaaagtagtt aatattaggg gagggcattt gcacctccct tgtaatatgt
     8161 ggaaaattga atattagaag aaggaggaga cttgatggaa aatcaacaat tattaggaac
     8221 agaacgaatt agtaaattac tactaaaata ttctatacca gctataatag gtatgttggt
     8281 aaatagttta tataatgtag tagatagaat ttttattgga aatataccag gggttggacc
     8341 acttgcaata acaggtcttg gtgtaacaat gccaataatg acaataatac ttgcatttgg
     8401 gatgttgata ggtataggaa ctactactac catatcaata aaacttgggc aaggtaaagt
     8461 agaagaggca agaaaactta taggaaatgc aatgacgtta tctgtaataa caggaattat
     8521 cataatgata cttggaatat tatttgcaaa taaaatactt acattgtttg gagcaagtga
     8581 aaatacttta atatatgcaa aatcatatat caatattata ttattaggta cagtagtaaa
     8641 tcttctttcc ttttctttaa accactcaat aagagctgat ggtagtccta aaatttcagc
     8701 aggaataatg atagtaggat gtttaactaa tatagtttta gactggatat taatatttgg
     8761 attcaatttg ggaatacaag gagctgcaat tgctacagta acttctcagg ctcttacagc
     8821 tattttaact atagggtatt atataagtgg aaaatcaaac ttacgattca gtaaatctaa
     8881 tctaaaatta gataaaaagc taataaaagc agtgtttgca ataggtatgt cgccatttgc
     8941 tatgcaactt gcagcaagtt tagttcaagt aatatcaaat attgcactta aaactcatgg
     9001 tggagattta gctatagggg ctatggctac tatttcttct attgcaatgg tatttttgat
     9061 gcctatcttt ggaataaatc aaggagcgca acctattata ggtttcaatt atggagctga
     9121 aaaatatgat agggttaaaa aagcatattt aggttcatta gtagtagcta ctataatttt
     9181 atgtatgggt atggtagtag ttatgctttt cccagaagct attataggta tatttaataa
     9241 agaccctgaa cttatgaata tatctgttaa tggattaaga atatacttac ttatgttgcc
     9301 aattgtaggc ttatcagtta caggaacaaa ctttatacaa tctataggaa aagcaaaaat
     9361 ggctatgtta ttgagccttt taagacaagt aattttattg ataccagcag tattaattct
     9421 tcctacattc ttaggtttac agggtgtatg gacagctcaa ccagtgtctg attttatagc
     9481 tacagtaatt acaggtatag ttgtatttag agaattaaaa agatatactc ctaaaactga
     9541 aaaattaaat gaaaatgaaa gactaaacga gataactact gagtaaaaat ataaattcag
     9601 ataattgagc aagaaattgc aaaaaatacg attatatgat aaaataaaac cgctgtcaag
     9661 gataagagaa attctaatta tggagtgatt ttatggaatt tacaaagaaa agcaaattaa
     9721 aaaatatgta tgtaaacatg aatgtagcta gaatgttgtc taagataaat gagtataagg
     9781 gtagacaact gctttacaaa aaacaaccaa aagaaatttt agaaaactta gaaaaaaaat
     9841 ctttagtaga ttgttctgaa tctacataca ttggaaatca aagagatagt agtaatttta
     9901 atctagaaaa gttgataagt aatgaagtta cacctagaag tagagaggaa ttgagtattg
     9961 ttgaatacag agatgtggta aaaactatta acagtgctta tgagagtatt cccatcagtt
    10021 cacaaacgat tcttgagctt catggatact tatataaatt ttcatctact agaggaggaa
    10081 gttataagtc agataatgat tttattgaac acaatttaaa acctagcgaa tttataagta
    10141 ttgattcaag tgtcaataaa gttgaaaaag ctgtagaaga aatatgtgaa gcttataata
    10201 cattgataga ggaagatgaa attgatatat taatattaat ttctgcattt gtgttagatt
    10261 tcatattgat acatccattc aaagaaggta atataaaaat ggcgagagta cttatcctat
    10321 tgttactaaa taaaaatggg tatgaagttg gaagatatat aagtttagga aagatatttg
    10381 atgatagttc atatgaatat tatagtaatt taaattattt gaaagcctca ataggtagtg
    10441 aaaaagcaga tatgaatgca tggattgagt attttttaga gacgatatta actgcatatg
    10501 agaagctgga tgatagttta aatatatctg ataagaaaag acaaacaaaa actagtagaa
    10561 tagagaaaat aataaattct acccttggat attttacaaa agaagatata agagatttat
    10621 gtcctgacat tccagaacct actataaata gagtttttaa taatctaaga aaacaagata
    10681 agatagaagt tgttgcaaga ggaagaagtg caaaatggaa gaaaaaatat taaaaaatgt
    10741 ttagataact aaaatggtat agtgttttac actataccat ttttaatgtc attagcatga
    10801 gcaataattt aacaacttaa gcaatatcaa ttttatttat tgattatttt tgaactgaaa
    10861 aatggcgtta tatggtatac ctcagtggta attaattttg gatgagaaga tataatatta
    10921 cagaatatat taggagtata tgtagaaaaa atataataaa agtgtgaggc ttagtaatta
    10981 acaaattatg tggcttcaca cttttttata aaataaagat acttagcaga agaatatttt
    11041 agttgatatg tttataagat ggtacttgtt tgggtaaaag tataaatagt aaaaaagtag
    11101 aagtaatgtg tataaaaacg taagttacat tctgtaataa tagttatttt ataattcaac
    11161 aaaagaatag aatgggtata tttgtgaaat aataatataa aataatctta ggagtgagct
    11221 tatgaagaaa aaattattat atataaatgt aaattcaaaa cctgaagact tatcttcaag
    11281 taaaactgta gcaagaaaat ttataaataa atttatggaa aaaaataaag attttgaggt
    11341 tgaagagata gacctttata aagaacatat accaagactt gagtatcagt actttgaaaa
    11401 aagaaatagc atagttagtg aagaaaatgc taaaaattta gacgataagg atagaaaaga
    11461 acttacaaag attagaaatc tttgtgacca atttgtgagt gcaagtgtat atgtaatagc
    11521 agcacctatg tggagtttat cttttccagc accacttaaa gagtatatag attgtataat
    11581 tcaagatgga aaaactatat cttttgaagg caatgataaa ccacaaggaa ttttaaatga
    11641 tattgataga agtatggtat atatacaatc ttctggtgga cacataccat gggtgttgaa
    11701 gccagttatg aataaagggt taaattatgt ggaaagtatt atgaaattta taggaattaa
    11761 aaaatttgat gaattgttag tagatggaac tggaactagt gaagaagaaa gacaagctgc
    11821 tatagaaaaa gcagctgaaa aaatagatgg tataatagat gggatgaaat tttaattata
    11881 atattttatt aaaaatataa aggaaaaacc tcttttaatg tagaaactta tattgaagga
    11941 ggcgagaatc atgcgagaag aaaaaagtaa tgaaaagtat gattgttatt ggtgtaatca
    12001 agagaataat ttctgtgtag aaataaaaga taatatagtc atgatagatg atggcactgg
    12061 tacgttaaaa caagccgttt tcatagggta taaacaaatc caaattaatt taaactgttc
    12121 acattgtcaa aacttgaata gaataaaatt aaatttgtag aattttaaaa tggaaattat
    12181 tgaaggaatt aaaaatagaa agtgttaata tttagactga aaattatatt ttatacaata
    12241 aaagtttatt taaataacat tgtattttaa tatttatttt cagtcttact ttattaaaat
    12301 tattttttgg ttatgtctgg aaaatatctg tctactaatt tttctgaaat attttttaat
    12361 tggtcagcca ttgaagtcag acttaataag gctatcaata aatcatcttt attttctgga
    12421 tttatactaa tattttcaaa attagactct ataaagtccg gtatattctt tctttgattt
    12481 tcttttatta acatgaattt ttcgtaacat gaaactaaat ctttttcact gaaattatcc
    12541 ttttcataca caccatgtaa gattctttta atttcttgta ttgtaagtgt atttttcata
    12601 gaataaataa ttatcatctg taaaatttgt tctttagtat acttttttcc cttaactggt
    12661 ttgattaatc cctctttgct gtaattattt atcatggttt tagtaagaat tttatcactt
    12721 tcaaatcttt tattggcaga aagcttattg tcaattagag aaataatttg atccatatat
    12781 aagtcaatag atggaatatc gtttgaagtt atatctgcat tatttagtgt ttctagtatt
    12841 atagtattaa gttcattatt catcatatgt tacctccata atatatataa tacactaaat
    12901 tagaaagtaa aacaatatta aggcttgtaa aaaaaacata aagtggtata attaaatata
    12961 taatatagta atggaaacta tatttgtgaa tctgattttt aaaatacata attgtaggag
    13021 gacgaaatgt atcaattttt tttaaagggt agagacccaa taagcagttt aactcatttt
    13081 ataggtgctt gtttatctct tttagcaact ataattttag tttttcaatc tgttacatta
    13141 caggaaactt ctctcttgat gatagtatct gtatcggtat ttggattttc actaattgct
    13201 ttatatagcg caagttcgta ttatcatttt ttaaagggga caccagaaca agaattgttt
    13261 tttagaaaag tagaccatgc aatgatttac gtattgattg ctggctctta tacaccaata
    13321 tgtctgaatt ttatggaaaa aaaagaaggc ataatatttg ttacagcaat atggatagtt
    13381 gcatttattg gtataataat aaaaatattt tggatggatg cacctagatg gttatctacc
    13441 agtatatatt tacttatggg gtgggcaatt gtttttgata taaatgcatt caattcaatt
    13501 cctaaggatt gccttagact attaattatg gaaggtgtat cttactctat aggtgcaatt
    13561 atttatataa ttaaaaagcc taatataagt cctgagtttg gatttcatga aatatttcat
    13621 atttttatta tgataggttc tttatttcac tttttagcag ttttattgta tgttttatag
    13681 ttttaaaaga tttgattaat tgatactaaa attctacaat gaataaaatg gaactgcctt
    13741 atatagatta tatcttcgac aataagacag ttccttatta taaatattaa attctactta
    13801 taataaaatt ttgataaaaa tagtccattc aaaatgccta ttccataatt cctttaatgt
    13861 acttttcatt tacgacatat agtttgaatt tatgtactat aattatatcc tctaaattct
    13921 ctgaaacact aacaattctg acacatttgt ttctaatttc tacaggtaaa tctaaatcat
    13981 ttgttaaatt cacatagtca tcatatttat acataaaatc atccctttac attaagatat
    14041 acttattata ctatatttta ataagttttt ttaaattatt acaatttttc ccgaaatttc
    14101 attttttata cataaaattt ccaaattagg taaagattaa attttttatt gtataaacaa
    14161 aagttacata taaaatattt tattcaattt tttaaataac tattg
//
LOCUS       DAMJVK010000099        13749 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.103, whole genome shotgun sequence.
ACCESSION   DAMJVK010000099 DAMJVK010000000
VERSION     DAMJVK010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13749
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.103"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            90..1883
                     /locus_tag="PC414_003060"
     CDS             90..1883
                     /locus_tag="PC414_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF2465440.1"
                     /translation="MNIKDRLSGLRKFMEEKNIDAYMIPSSDNHQSEYVGDYFKSREF
                     ISGFNGSAGTVIVTKDEAGLWTDGRYFIQAESQLEGSTIKLFKMGQEGCPTTDEYLYK
                     NIPEGGTLGFDGRVISAREGATLAEKLSKKGIKIEYQYDLIDSIWPDRPALSDSKAFL
                     LDVKYCGESFSSKLARLREKMSEKGTSTHVITTLDDIAWLFNIRGGDVKYNPVVLSYA
                     VITLKEVYLFVDESKLNEEILNELAKENVQIKPYNDVYEFVKNIDKTEKVLLDGTKLS
                     YTIYNNIPCEVEKVDEFNPVMFFKAQKNEVELENIRNSHVKDGVAFTKFMYWLKKNVG
                     KMEITEISATQKLEDLRREQEGFFEPSFNTIAAYKEHAAMMHYSATPESNYKLEAEGL
                     FLVDSGGQYYDGTTDITRTTVLGPISDELKLHFTSVARGMINLSKAKFLHGCRGYNLD
                     ILSRSCMWNMGIDYQCGTGHGIGFVLNVHEAPNGFRWRVVPERFDSAVLEEGMVTTNE
                     PGIYIEGSHGIRTENEIVVRKAEKNFYGQFMEFEVVTLAPIDLDGIAPELMNKDEKDY
                     LNWYHKLVYDKISPFLTDEEREWLKVYTRAI"
     gene            complement(1964..3346)
                     /locus_tag="PC414_003061"
     CDS             complement(1964..3346)
                     /locus_tag="PC414_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HDF2465441.1"
                     /translation="MSVLKTQEKLKQPRDLYFCCSIFTFVGLAYYGMKLVLLLFLAEQ
                     VSKGGLGLTPAESASMLASFLAWTYFSPVIGGWVSDRFLGPKNCIILGMLMVSIGYFL
                     GYIANSKMDINIMIFLSGLGIGFYKGNLHTMVGNLYTNDDPRKDGAFSITYMATNLGT
                     LFGPLICGLVANEWFAMKSGLSISMYGYRYVFLFSSITVLIGLFFFIFGNRKFYKPLN
                     NDPINGRESIRKSLEYKKMLASRPLTLIEKKRIIVILVLAFFTVFFWIAYNQASMSIA
                     LYTKEHINLTVGNFQIPTSWIDSYNGLLCVILGPISALVWVKLSQTKKGDLSVPKKMS
                     LGFILLAIAFLFMIVAVIQTGTDPNSIHKASVFWVVGFLTFQSIGEICFSPVGYGMVN
                     KLSPEKYISFLMGVWFLGKFAANKLSGYTQAIIDQLGMLQVFIVIPSFLLIFGIILLI
                     MNKKLVELSN"
     gene            3604..3951
                     /locus_tag="PC414_003062"
     CDS             3604..3951
                     /locus_tag="PC414_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDF2465442.1"
                     /translation="MIRKKSELQVEVKENLNGGIGKVKLENIIQNEELKGKGRLFKRV
                     ALPVGSSIGVHDHTTDFEVYYILKGKGKVFDNGEFVEVNEGDVVYTADGEKHSIENIG
                     EEDLEFVALVLYM"
     gene            3969..5003
                     /locus_tag="PC414_003063"
     CDS             3969..5003
                     /locus_tag="PC414_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465443.1"
                     /translation="MTGKIEEIKDKINRFCNSNLNQEYKDISFKILQNLLDNNKEIIH
                     SSRADIWSSAILNIVLEQNLLYNKKHPLHITKKEFSKQIGVSLNTINNKSSLIKDVCS
                     IDFLNQDTIAISNVEFWVKETNSKSKSVDLDLEKKKILYKRYIKLSQDANSDIESIRY
                     MEEAVNIGKSMITKEDKELGFWKGLSTRAYMIALESLARKLESINNLKESKKVLEYLI
                     EINPSDEQGIRYKLLNVLIRLNDRTSINKLFELYKEEKSATWMYSKALYYFKNKSMFL
                     ANDSIKVARSKNKYVGLYLLDWRNAFGREFVTEEEKAEAVYYYDENIVIWNEVKGSMD
                     WLLKKMLEFS"
     gene            complement(5052..6101)
                     /locus_tag="PC414_003064"
     CDS             complement(5052..6101)
                     /locus_tag="PC414_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2465444.1"
                     /translation="MEINYKKRNFKSFAKRKNIEISVQRYLIDALSYMALGLFSSLLI
                     GTIFNTLGDNFNISLFTEVISPLAKQVTGPAIAVAIAYGLQAPPLVLFSCALVGACGN
                     ELGGPVGAFVATLFAVEVGKLVSKETKIDILVTPAVTILIGVLVAELIGPAVSAFMTS
                     FGNIIMQATTMQPIIMGALVSALVGIALTLPISSAAICMMLSLGGLAGGAATVGCCCQ
                     MVGFAVMSFRENGVGGLVAQGLGTSMLQVPNIIKNWKIWIPPTVASILLGPLSTTIFK
                     MENIPIGSGMGTCGLVGQFGTVTAMQSAGKGGISMWVGILLLNFILPAIVTLLISEFM
                     RKKGLIKPEDLKLDV"
     gene            6376..7911
                     /locus_tag="PC414_003065"
     CDS             6376..7911
                     /locus_tag="PC414_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465445.1"
                     /translation="MIFVKNKKYRKIAIIISIPILLVAQYYLFKFGILRPMVKGVEIE
                     IVDGEYVKDIDKYVVKLNETVTLSTGEYIKVPSYAKNPNIWFNVLDNSGTLKIDGNKM
                     TALKVGYSSVGIMKNSRVLKKATIKVVDPEVESLDMEINGDLKHVGDSATIESTIEVD
                     YDKFKKSYKPVYKSSNENILKIKGNKVSAVGVGKATIYAKCGNKEIERTFRIQARVAE
                     INMDDIKIEVDQNEKLKPDIITSPPGLEPPKIYYELVDSKLPVERAISLSSNGNVVGL
                     REGKDRVRITCGDKSKVITVTVVKETITNDYIQNLVGSHKIVNNKVIIKLEWDYMKDV
                     FDYDIYLKDKLSGESFKKIKSITINESDLGKSNKVYATIEVDMKGNQNIDFDIYVIGK
                     KDSDTTKPSNVINITNGNGNSNNDNNIKDMRVENISANVDRDNNVIRVNWSSISHKDC
                     TYSIYVKNNTNGDGEFTLYQSDIQGNEHTISIPEGDLNLDIYVVANYNGNSSQNSDVI
                     NVR"
     gene            complement(8096..8782)
                     /locus_tag="PC414_003066"
     CDS             complement(8096..8782)
                     /locus_tag="PC414_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HDF2465446.1"
                     /translation="MKTIGLIGGMSWESTITYYQVINTVIKERLGGLHSSKCILYSVD
                     FQEIEECQSSGNWEKSAKILADAAIKLQEAGADFIVICTNTMHKVSDKIQESIHIPLL
                     HIADVTATVLREKEIKKVALLGTKYTMEQDFYKNVIINNGIEVLIPNEEDRIIVNDTI
                     FNELCLGIISESSKKAFLSIIDKLGKQGAEAVILGCTEIGLLIKQNDTSIPLFDTTVI
                     HAIEAALSSI"
     gene            8907..10289
                     /locus_tag="PC414_003067"
     CDS             8907..10289
                     /locus_tag="PC414_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF2465447.1"
                     /translation="MPINSFENYPMTWKPKLDNRKPPIYKTLAMLLEEDIKRGNLNPG
                     DKLPPQRELADFLDLNLSTITRAFKLCEEKGLICAKVGKGTFISSDVNVSNTLLYQTE
                     SKDIIELGTVHPPYEQNTYIIDFIKNVLKQPEMDRFLQYMSPSGTYMQKKSIAKWIER
                     NNVYTSEENILLSTGGQNAICATLLGLFKAGDRIATDSLSFSGIKSIAKMIGIQLVPI
                     PQENNEISIEYLESYCKNENIKGIYLIPDYHNPTTHTMSDLSRKKIAKIAKQYNIIII
                     EDAINSIFRDGIQTPIFSLASDNTIYIFSTSKFLCAGLRVAFVVAPKRYIENLENALY
                     NMNLMVSPFTAEIVHRLLYSPIIDKIIEEKRDAIIERNEVADKVLSDYNLIGDKNCSF
                     RWLLLPDELDGKSFEISAKNLGVQVYCAERFSVGNSTVPKAVRICVTAPKDVEELEKG
                     LNIIKSLLKS"
     gene            10691..11959
                     /gene="eam"
                     /locus_tag="PC414_003068"
     CDS             10691..11959
                     /gene="eam"
                     /locus_tag="PC414_003068"
                     /EC_number="5.4.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 2,3-aminomutase"
                     /protein_id="HDF2465448.1"
                     /translation="MNEQTRISLERAAELKSKIDDYIQARKTINRGLEKEEEINKRKQ
                     KILSILNGTEEDWNNYKWQLSNRITDVDTLSKIITLTKKEKEYIKEVGTQFRWAISPY
                     YLSLIDPEDTCDPIKLLSIPTHIELEDEQEDLDPMGEEYTNPAGCITRRYPDRLIINV
                     TNECAMYCRHCQRRRNIGQQDSHKSKAIIQESIDYIRENEEIRDVLVTGGDALTLKDD
                     YLEWILSQLKEIPHVDYVRLGTRTLVTMPQRITDEFCNMLKKYHPVYINTHFNHPMEI
                     TKESKEACEKLANAGVPLGNQAVLLNGINNDKFVMRCLNQELLKIRVKPYYIFQSKHV
                     KGTKHFNTSVDDGLEIMEYLRGYTSGMAIPTYIVNAPKGGGKTPLLPQYLVSKGTDYV
                     MLRTWEGKVIKMEDEPAVDIKKLIKEQAQD"
     gene            complement(12052..12408)
                     /locus_tag="PC414_003069"
     CDS             complement(12052..12408)
                     /locus_tag="PC414_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465449.1"
                     /translation="MKKALISLLVLSCLLLTAPVNESSYADSQKNVPVSQNTKLTQEQ
                     AKELLVKYNNEVDYIYQGNASDFEALKAKNLNGYVFLPDADGDIGYFVNENTSEIYYF
                     HPSGYLELVNQQQNNK"
     gene            complement(12559..13653)
                     /locus_tag="PC414_003070"
     CDS             complement(12559..13653)
                     /locus_tag="PC414_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDF2465450.1"
                     /translation="MQDKKIYLNIVLTVVVSYILIKVIDNYKYFFGVISLLMSLLTPF
                     IIAFVLAYIFNPLVKFLESKLNFKRIYSLLLTYGVLIVILISFILFTVPSIVNSLADL
                     VAQIPTYIDKTEQFLFDLGKSLQSVDPNTLKEYGDKIMSVMPKFSNLLIGSLGGIFST
                     TFSVGKFIVQFLLGFIICFYILLEKEKFLLFTKKVVYISLGKKYGDFIIELCQSLNLN
                     IGKYFTGKILDSFIVGVLSGIGLYFLKSEYALLFGTLMGVMNMVPYFGPVIGMAPVVI
                     INLFSNPTIALTSLIYLIIIQQVEVTFIEPKIVGGQLGLSPFFTILAVTVGGGFFGIP
                     GMILSAPVMGVIKIYFCEFVNRRHDKIQME"
BASE COUNT         5389 a         1734 c         2175 g         4451 t
ORIGIN      
        1 atataaatta tacaaaaaat atttatgtaa taaactttac atacttatat atttagttat
       61 tcaaactatt atttcgagga ggtcttgttg tgaatataaa agatagatta agtggattaa
      121 gaaaatttat ggaagaaaaa aatatagatg cctatatgat tccatcttct gataatcacc
      181 aaagtgaata tgtaggagat tacttcaaat caagagaatt tatatcaggg tttaatggtt
      241 cagcagggac ggttattgtg actaaagacg aagctgggtt atggacagat ggaagatatt
      301 ttatacaggc agaatctcaa ttagaaggaa gtacaataaa attattcaaa atgggacaag
      361 aaggttgccc aactacagat gaatatttat ataaaaatat tcctgaagga ggaactttag
      421 gatttgatgg tagagttata tctgctagag aaggagctac attagctgaa aaattatcta
      481 aaaaaggtat aaagatagaa tatcagtatg atttaataga tagtatatgg ccagatagac
      541 ctgctttatc agatagtaaa gcatttttat tagatgttaa atattgtgga gaaagtttta
      601 gctcaaaatt agctagatta agagaaaaaa tgagtgaaaa aggtacttct actcatgtaa
      661 taactactct tgatgatata gcatggttat tcaatataag aggtggagat gttaaatata
      721 atccagttgt actttcttat gcagtaataa ctttaaaaga agtttatcta tttgttgatg
      781 aaagtaagtt aaatgaagaa atactaaatg aactagctaa ggaaaatgtt caaataaagc
      841 catataatga tgtgtatgaa tttgtaaaaa atatagacaa aactgaaaaa gttttattag
      901 atggaactaa gcttagttat acaatttaca ataatatacc ttgtgaagtt gagaaagttg
      961 atgagttcaa tccagtaatg ttttttaaag ctcaaaaaaa tgaggtagaa ttagaaaata
     1021 taagaaattc acatgtaaaa gatggtgtag ctttcactaa atttatgtac tggttaaaga
     1081 aaaatgtagg taagatggaa atcactgaaa taagtgctac tcaaaaatta gaggacttaa
     1141 gaagagagca agaaggtttc tttgagccaa gtttcaatac aattgctgca tataaagagc
     1201 atgctgcaat gatgcactat agtgcaactc cagagtcaaa ttataaatta gaggcagaag
     1261 gattattttt agtagattca ggtggacaat attatgatgg aactactgat ataactagga
     1321 ctacagtttt aggacctata agtgatgaat taaaactaca ctttacttct gtagcaagag
     1381 gaatgataaa tctttctaag gctaagttct tgcatggttg tagaggatat aacttagaca
     1441 tactatctag aagttgcatg tggaatatgg gaattgatta tcaatgtgga actggacatg
     1501 gtataggatt tgtgctaaat gttcacgaag ctccaaatgg atttagatgg agagtagtac
     1561 ctgaaagatt tgatagtgca gttcttgaag aaggaatggt aactactaat gaaccaggta
     1621 tatatataga aggttctcat ggtataagaa cagagaatga aattgtagtt agaaaagctg
     1681 aaaagaattt ctatggtcaa tttatggaat ttgaagttgt tacgttagct cctattgact
     1741 tagatggtat agcaccagaa ttaatgaata aggatgaaaa agattattta aattggtatc
     1801 ataagttagt ttatgataaa atatcacctt tcttaactga tgaagaaaga gaatggttaa
     1861 aagtttacac tagagctata tagtttgtac tagatacaat aaaaatcgag gtattataat
     1921 gcctcgattt tttattgtat attttattaa ctttaaaaaa tttttaattt gataattcta
     1981 ctaatttttt attcattata agtagtataa ttccaaatat caataaaaat gatggtatca
     2041 ctatgaagac ttgtaacata ccaagttggt ctataatagc ttgcgtatag ccagataatt
     2101 tatttgctgc aaatttgcct aaaaaccaaa cacccattag gaatgaaata tacttttcag
     2161 gcgaaagttt atttaccata ccatacccga ctggagaaaa gcaaatttct cctatacttt
     2221 ggaatgttaa aaatccaact acccaaaata cacttgcttt gtgaattgaa ttagggtctg
     2281 ttcctgtttg tataactgct actatcataa acaaaaatgc tatggctaat agtataaaac
     2341 ctaagctcat tttttttggc acacttaaat cgcctttttt agtttgagat aattttaccc
     2401 atactaaagc agagataggt ccaagaataa cacaaaggag accattgtat gaatctatcc
     2461 atgaagtagg aatttgaaaa ttgccaacag taagatttat gtgttccttt gtatagagtg
     2521 caatagacat agaggcttgg ttatatgcta tccaaaaaaa tactgtgaaa aaagctaaca
     2581 ccaatattac tattattctt tttttctcaa tcaaagttaa aggtcttgaa gcgagcattt
     2641 tcttatattc tagtgatttt cttatagact ctctcccgtt aatagggtca ttattcaaag
     2701 gtttataaaa ctttctgttt ccaaatataa agaagaataa acctattaga accgtaattg
     2761 atgaaaataa aaatacatat ctatatccat acatacttat agataatccg cttttcatag
     2821 caaaccattc attagcaact aaaccgcata ttaaagggcc aaataaagta cctagattag
     2881 tagccatata cgtaatagag aaagctccat cttttcttgg gtcatcattg gtatataagt
     2941 ttccaaccat agtatgtaag tttcctttat aaaaacctat tcctaatcca gataaaaata
     3001 tcatgatgtt tatatccatc ttactatttg ctatgtatcc tagaaaataa ccaattgaaa
     3061 ccattagcat tccaagtata atacagtttt tgggacctaa aaatctatca ctgacccatc
     3121 caccaataac tggagagaaa taagtccatg ctaaaaaaga tgcaagcatt gatgcggatt
     3181 ctgctggtgt taaccctaaa ccacctttgg agacttgttc agctaaaaac aataataata
     3241 ccaatttcat accatagtat gcaagtccta caaaggtaaa tattgaacag cagaaatata
     3301 aatctctagg ttgtttaagt ttttcttgcg tttttaaaac tgacatatgt cgagaacctc
     3361 cagataaata gtttaacaaa attttaccat actatatgag tatatgtttt tttcatatag
     3421 acgtcaactt gtaagataat ttaattaagt cgaaatatat gtaaaaatag tatcattttt
     3481 gtgtattcag taatattttt ttatgataat atatgttatt attatattaa aatacaaaca
     3541 tatttatgga aaggatatgc caatttagta gttagattga ttgtttggca ggtgggatga
     3601 gctatgataa gaaagaaatc tgaattacaa gtagaagtaa aagaaaattt aaatggtggt
     3661 ataggaaagg ttaagcttga aaacataata caaaatgaag agcttaaagg aaaaggaaga
     3721 ctatttaaaa gagtagcttt accagtagga agttcaattg gtgtgcatga ccatacaaca
     3781 gattttgaag tatattatat actaaaggga aaaggtaaag tatttgataa tggggaattt
     3841 gtagaagtaa atgagggtga tgtagtgtat acagctgatg gcgaaaaaca ttctatagaa
     3901 aatataggag aagaagatct tgagtttgta gcactagttt tatatatgta aaatatggag
     3961 gaggagtcat gacaggcaag atagaagaaa ttaaggacaa gataaataga ttttgcaact
     4021 ccaatttaaa tcaagaatac aaagatataa gctttaaaat tttgcaaaac cttttagata
     4081 ataataaaga gataattcat tcttctagag ctgatatatg gtcttcagct atactaaata
     4141 tagttttaga acaaaatctt ttatacaata agaagcatcc actacatata acaaaaaaag
     4201 agttttcaaa acaaataggt gtttctttaa atactataaa taataaatcg tctttaataa
     4261 aagatgtttg tagtattgac tttttaaatc aagatactat agctatttct aatgtagaat
     4321 tttgggtaaa agagactaat tccaaatcaa agtctgtaga tttagattta gaaaagaaga
     4381 aaatactata taaaagatat attaagctat cacaagatgc gaatagtgat attgaatcaa
     4441 ttagatatat ggaggaagct gtaaatatag gtaaaagtat gattactaaa gaagataaag
     4501 aacttggatt ttggaaaggt ttatctacca gagcctatat gatagcactt gaatcccttg
     4561 ctagaaagtt agaaagtatt aataatctaa aggagtccaa aaaagtatta gaatatttaa
     4621 tagaaattaa tcctagtgat gagcagggaa taagatacaa attattaaat gtattaatta
     4681 gattaaatga tagaacttca ataaataaat tatttgaact ttataaagaa gaaaaatctg
     4741 ctacatggat gtacagtaaa gctctttatt actttaaaaa taaaagtatg tttttggcaa
     4801 atgattctat taaggttgct agaagtaaaa ataagtacgt tggattatat ttgttagatt
     4861 ggcgaaatgc ttttggtaga gaatttgtta cagaggaaga aaaggcagaa gctgtctatt
     4921 actatgatga aaacatagtg atttggaatg aagttaaagg ttctatggat tggctattaa
     4981 aaaaaatgtt ggaattttca taaaaaaaac tactccttac ttttatataa gtcaaaggag
     5041 tagtttttaa tttatacatc tagttttaaa tcttcaggtt ttattagacc tttttttctc
     5101 ataaattctg aaatcaacaa agttacaata gcaggaagta taaaattaag taaaagtata
     5161 ccaacccaca tagatatacc tccttttcca gcactttgca tagcagttac tgttccaaat
     5221 tgtccaacca atccacaagt acccatacca gaaccaatag gaatattctc cattttgaat
     5281 attgttgtag ataatggtcc aagtaatatt gaagctacag taggtggtat ccaaattttc
     5341 caatttttta ttatattagg tacttgcaac atagatgtcc caagaccttg tgccacaagg
     5401 ccaccaactc cattttctct aaagctcata acagcaaagc ctaccatttg gcaacaacaa
     5461 cctacagttg ctgcaccacc agcaagacca cctaaactta acatcataca aatagctgca
     5521 ctacttatag gtaatgtaag agctattcca acaagtgcag agactaaagc tcccattatt
     5581 ataggttgca tagttgtagc ttgcattatt atatttccaa atgaagtcat aaaagcagaa
     5641 acagctggac caataagttc agctacaagt actccaatta gtattgttac agcaggtgta
     5701 accaatatat ctatttttgt ttcttttgaa actaattttc caacctctac agcaaaaagt
     5761 gtagcgacaa atgctccaac aggtcctcct aattcattac cacaagctcc aacaagtgca
     5821 caagaaaata gtacaagtgg aggtgcttga agtccatacg caatagccac tgctatagct
     5881 ggaccagtaa cctgtttagc aagagggctt ataacttcag taaaaagaga aatattgaaa
     5941 ttatctccta atgtattgaa aattgttcca attaaaaggg acgaaaaaag accgagtgcc
     6001 atataactta gtgcgtcaat aagatacctt tgaacagaaa tttcaatatt ttttctttta
     6061 gcaaatgatt tgaaatttct ttttttataa ttgatttcca atttaaaatc ctcctcatat
     6121 aatataagta taatttcttt ttaattaatt atataacttt tcagctgaca agacaaatgt
     6181 tatttcacta aaattagact attgtaatag taagaaatta acattaattt tagttttaaa
     6241 ttttaaattt atggtttata attagtatag ggatattctg aaatataata attttccagt
     6301 ttatcccaat atttaactta ggtagattgc ttacaaaaca aatgattata tattaggcta
     6361 aaaggaagtg gatagatgat atttgtaaaa aataagaaat acagaaaaat tgccataatt
     6421 atatctattc ctatattgct tgttgcgcag tactaccttt ttaaatttgg aatacttaga
     6481 ccaatggtaa agggagtaga aattgaaatt gtagatgggg aatatgttaa agatatagat
     6541 aaatatgtag taaaattaaa tgaaacagtt actttgtcta caggtgaata tattaaggtt
     6601 ccttcgtatg ctaaaaatcc aaatatttgg tttaatgtat tggataactc agggacttta
     6661 aaaatagatg gaaataaaat gacagcatta aaggttgggt attcttctgt gggtatcatg
     6721 aaaaattcaa gagtattaaa aaaggctact ataaaagtag tagacccaga ggtagaatca
     6781 ctagatatgg agattaatgg ggatttaaaa catgttggtg atagtgctac tatagaaagt
     6841 acaatagaag ttgattatga taagtttaaa aaatcatata agccagttta taagagtagc
     6901 aatgaaaata ttttaaaaat caaaggaaat aaagtaagtg cagttggagt tggtaaagct
     6961 acaatatatg ctaagtgtgg aaataaagaa attgagagaa catttaggat tcaagctaga
     7021 gttgctgaaa taaatatgga tgatataaaa atagaagttg accagaatga aaaattgaaa
     7081 ccagatataa taacttctcc gcctggttta gaaccaccaa aaatatatta tgagttagta
     7141 gattcaaaat tacctgtaga aagagcaata agtttaagta gtaatggaaa tgttgtaggg
     7201 cttagagaag gtaaagatag ggtgagaata acttgtggag ataaatctaa agtaataaca
     7261 gtaacagtag tcaaagaaac tataacgaat gactatatac aaaacttggt tggttcacat
     7321 aaaatagtaa acaataaagt tattattaaa cttgaatggg attatatgaa agatgtcttt
     7381 gattatgata tatatcttaa agataagttg tcaggagaat catttaagaa aattaaatct
     7441 ataactataa atgaaagtga tttaggtaag tctaacaagg tatatgcaac tatagaagta
     7501 gatatgaagg gtaatcaaaa tatagatttt gatatttatg tcatagggaa aaaggattca
     7561 gatacaacta agccaagtaa tgtaataaat attaccaatg gaaatggaaa ttcaaataat
     7621 gataataata ttaaagatat gagggtagaa aatatatcag ctaatgttga tagagataat
     7681 aatgtcatta gagttaactg gagtagtata tctcataagg actgtactta cagtatatat
     7741 gtaaaaaata atactaatgg agatggagaa tttactctat atcaaagtga tatacaggga
     7801 aatgagcata ctataagtat tcctgaagga gatttaaatc tagatattta tgttgttgca
     7861 aattataatg gaaatagctc tcaaaatagt gatgttataa atgtaagata gatttgtata
     7921 caaattgaaa gtgttgagtt tatttaaata ttaaattaag aaaaaggata gtatagggta
     7981 attatactat ccttttttgt tacaatatat aggtttaaaa tctctagtaa tataaattat
     8041 aaaaagtctt atttacacaa atctatagat atttttttta ttaatcaata ttgtcttaaa
     8101 ttgaacttag tgcggcttct attgcatgta ttactgtcgt atcaaatagt ggaatagaag
     8161 tgtcattttg tttaattaaa agacctattt ctgtacatcc taatataaca gcctctgcac
     8221 cttgtttgcc aagcttatct ataatagata agaaagcttt ttttgaagat tcagatataa
     8281 ttcctaagca aagttcatta aatattgtgt catttactat tatacggtct tcttcatttg
     8341 gaattaagac ttcaattcca ttattaataa ttacattttt ataaaaatct tgttccattg
     8401 tatatttagt acctaataag gccacttttt ttatttcttt ttcccttaat accgtagcag
     8461 ttacatctgc tatgtgaagt aatggaatat gtatactttc ttgtatttta tctgatacct
     8521 tatgcatagt attagtacag ataacaataa aatctgcccc tgcctcttgt aatttaattg
     8581 cagcatcagc taaaattttg gcgctttttt cccaatttcc actagattgg cattcttcaa
     8641 tttcttgaaa atctacactg taaagtatac atttagaaga gtgtagtcca cctaatcttt
     8701 cttttattac tgtattaata acttgataat atgtaatggt actttcccaa ctcataccac
     8761 caattaagcc tatagtcttc atattatacc tctcttattt atttattttg aaatagctat
     8821 ttacaaatac aagtatagca tctaaaatag aagcatacaa tgataaaatt gtatggagta
     8881 caattataat taagaggagt aattatatgc caataaattc ttttgaaaac tatcctatga
     8941 catggaaacc aaaattagat aaccgaaaac caccaatata taaaacttta gcaatgttat
     9001 tagaagaaga tattaaaaga ggaaatttaa atcctggaga taaactgcct ccacaacggg
     9061 aattagcaga ttttctagat ttaaacttaa gtacaataac acgagcattt aaactttgcg
     9121 aagaaaaggg actaatatgt gcaaaagtgg gaaagggaac attcatttct tctgatgtaa
     9181 atgtaagtaa tacattatta tatcaaacgg aatcaaaaga cataattgaa ttagggacag
     9241 ttcatcctcc gtatgagcaa aatacatata ttattgactt tattaaaaat gtactaaaac
     9301 aaccagaaat ggataggttt ttacaataca tgtctccatc tggcacatat atgcaaaaaa
     9361 agtcaattgc aaaatggata gaaagaaata atgtatacac cagtgaagaa aatatattat
     9421 tatctacagg agggcaaaat gctatatgtg caactttact aggattattt aaagctggtg
     9481 atagaattgc tacagattct ttaagttttt ctggaattaa gtcaatagct aaaatgatag
     9541 gtattcaatt agtcccaatt cctcaagaaa ataatgaaat atcgattgaa tatcttgaaa
     9601 gttattgtaa aaatgaaaat ataaaaggca tatatcttat accagattat cataatccaa
     9661 cgacacatac tatgtctgat ttatctcgta aaaagatagc caagatagca aagcaatata
     9721 atataataat tattgaagat gctataaata gcatttttag agatggtata caaactccca
     9781 tattttcttt agctagtgat aatactatat atatttttag tacatctaaa tttttatgtg
     9841 ctggtcttag agtagcattt gtagttgctc caaaacgata tatagaaaat ttagaaaatg
     9901 cactatataa tatgaatcta atggtttctc catttacagc agaaatagta caccgtttat
     9961 tatattctcc tattatagat aaaattatag aagagaaaag agatgcaatt atagaaagaa
    10021 atgaagttgc tgataaagtt ttgtcagatt ataatttgat tggtgataag aattgcagtt
    10081 ttcgttggtt acttcttcct gatgaattgg atggaaaatc atttgaaatt tctgcaaaaa
    10141 atctaggagt tcaagtatat tgtgctgaac gattttcagt aggaaactct acagttccaa
    10201 aagcagttcg tatttgtgta acagctccaa aagatgtaga agaattagaa aaagggctta
    10261 atataataaa gtcacttctt aagagttaaa tttattttac tattatttaa ttaaataaat
    10321 aaaaaggcgg agaaatttat tataataaac ctccgctttt attatttaat gaaatacttt
    10381 actttttatt aaaaaattta attttaataa tcttgaaaat ataatcgttt tatggtaaaa
    10441 taattcttaa aaatagaaaa aattaatcat ttgttaaaaa aaatgcataa tttattgtaa
    10501 tttattgacg aaggttgaaa aatattcata aatgtgcgaa ataacagaaa aaatgcaaaa
    10561 attaattcaa tttcatttgt tataaaatct atatggcaaa attaaatctg cctatcagaa
    10621 tgatataaac acaccagaaa ggaggacgct atcaaataat acaagtagtt aactttgata
    10681 gcaaaaaatt atgaatgaac aaactagaat atccttagag agagctgctg aattaaaatc
    10741 aaaaattgat gattatattc aggctagaaa aacgattaac agaggtcttg aaaaagaaga
    10801 agagataaat aaacgaaaac agaaaatatt aagtatctta aatggaactg aagaggattg
    10861 gaataactac aaatggcaat tatcaaatag aataacagat gtagatactt tatcaaaaat
    10921 tataactcta actaaaaaag aaaaagaata tataaaagag gttggtactc aatttagatg
    10981 ggcaatatct ccatattatt tgagtcttat agacccagaa gatacatgtg acccaataaa
    11041 attactgtct ataccaacac atatagagtt ggaagatgaa caagaagatt tggacccaat
    11101 gggagaagag tatacaaacc cagcaggatg tataactaga agatacccgg atagattaat
    11161 aataaatgta acaaatgagt gtgctatgta ttgtagacac tgtcagagaa gaagaaatat
    11221 tggacaacaa gattctcata agtcaaaagc tattatccaa gaatctatag actatatcag
    11281 agaaaatgaa gaaataagag atgtactagt aactggtgga gatgctctta cattaaaaga
    11341 tgattattta gagtggattc ttagccaact taaagagata ccacatgttg attatgttag
    11401 attaggtact aggactcttg ttacaatgcc acaaagaatc acagatgaat tttgcaatat
    11461 gctaaaaaaa tatcaccctg tatatataaa tactcatttt aatcatccaa tggaaataac
    11521 taaggaatct aaagaagctt gtgaaaagtt agcaaatgca ggagttccat taggaaatca
    11581 ggcagtatta ttaaatggaa taaataatga taaatttgta atgagatgtt taaatcaaga
    11641 attactgaaa ataagagtaa aaccttatta tatattccaa agtaaacatg taaagggaac
    11701 aaaacatttc aatacatcag tagatgatgg tcttgaaatc atggagtatt taagaggata
    11761 tacatcagga atggctatac caacatatat agtaaatgct ccaaaaggag gcggaaagac
    11821 tcctttgctt cctcaatacc ttgtatcaaa aggaacagat tacgttatgc ttagaacatg
    11881 ggaaggaaaa gttataaaaa tggaagatga acctgctgta gatataaaga aacttataaa
    11941 agaacaagcg caggattaaa aaagtattta aagtttataa aaagaaacaa ctttaaaata
    12001 aaaagaaaat ggatgtcaaa tttatatcaa tttgcatcca ttttttatta gttatttatt
    12061 attttgttgt tgattaacta attcaagata tccacttggg tggaagtaat atatctcaga
    12121 agtattttca tttacaaagt aaccaatatc accatctgca tctggtaaaa atacataacc
    12181 attaagattt ttagctttta atgcttcaaa atcactagca tttccttgat atatgtaatc
    12241 aacttcattg ttgtatttta ctaaaagttc tttagcttgt tcttgagtca atttagtatt
    12301 ttggcttaca ggaacattct tttgagaatc tgcataagaa gattcattta ctggtgcagt
    12361 taataacaaa caagataaaa ctaataaaga tataagtgct tttttcatac taaaaccctc
    12421 tttcatgttt aattatataa gattcattac ttaataatat tgtatgtttc ctataattaa
    12481 atattaccat taaagagaat aatattacag ttacacagct gtaacttttg attacaaaat
    12541 cgtaattaaa ttgtattttt attccatttg tattttatca tgtctacgat ttacaaactc
    12601 acagaagtat atctttataa cgcccatcac aggagcagat agaatcatac caggtatacc
    12661 aaagaatcct ccaccaacag taactgctag gattgtaaag aatgggctaa gacctagttg
    12721 tccacctacg atttttggct ctataaatgt aacttcaact tgttgaatta ttattaagta
    12781 aattaaagaa gttaaagcaa ttgttggatt ggagaataaa ttaattatta caactggtgc
    12841 catacctatt actggaccaa aatatggaac catattcatg acacccataa gtgtaccaaa
    12901 taatagtgca tattcagatt ttaaaaagta aagaccaatt ccagaaagta ctccaactat
    12961 aaaagaatct aaaatcttac ctgtaaagta tttaccaata tttaaattta agctttgaca
    13021 tagttctatt ataaagtctc catatttttt acctaaggaa atataaacta ctttttttgt
    13081 aaacagtaag aatttttctt tttctaataa tatatagaaa caaattataa aacctaaaag
    13141 aaattgtact ataaattttc caactgaaaa tgtagtgcta aatatcccac caagtgaacc
    13201 aataagcaag ttactaaatt taggcattac actcataatt ttgtcaccat actcttttaa
    13261 tgtattaggg tcaactgatt gtaaactctt gcctaagtcg aataaaaact gctctgtttt
    13321 atctatgtat gtaggaattt gagcaactaa atcagctaaa ctatttacta tggatggaac
    13381 tgtaaataat ataaacgaaa ttagtataac tatgagtaca ccataagtaa gaagtaacga
    13441 atatattctc ttaaaattta atttactttc taagaacttt actagtgggt tgaatatata
    13501 tgctaataca aatgctatta taaatggtgt tagtagtgac attaataaac ttatcactcc
    13561 aaaaaaatat ttgtaattat caataacttt aatcaagata tatgaaacaa ctacagtgag
    13621 tacaatattt agataaattt ttttatcttg caaaataaca caacctttct tatatttatt
    13681 aaatttagat atacttaaat ctaagttaaa gttaatattt atagcttatt atacaataaa
    13741 aagcagtat
//
LOCUS       DAMJVK010000100        13710 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.104, whole genome shotgun sequence.
ACCESSION   DAMJVK010000100 DAMJVK010000000
VERSION     DAMJVK010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13710
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.104"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(82..543)
                     /locus_tag="PC414_003071"
     CDS             complement(82..543)
                     /locus_tag="PC414_003071"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HDF2465451.1"
                     /translation="MIYEGKLIGKDLKIGIINSRFNEFITSKLLSGAEDCLLRHDVSP
                     ENIEIVWVPGAFEIPLVAQKMAKSGKYDAIICLGCVIRGATSHYDYVCSEVSKGIAKV
                     SLDSELPVIFGIVTTENIEQAIERAGTKAGNKGYDCAMNALEMANLFKSLN"
     gene            complement(648..1841)
                     /locus_tag="PC414_003072"
     CDS             complement(648..1841)
                     /locus_tag="PC414_003072"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HDF2465452.1"
                     /translation="MFNTIEEAIEDIKNGKMVIVVDDESRENEGDLLMAAEMATPESI
                     NFMATYGRGLICLPATEKKFKSLNIPLMVRENTDTFQTAFTVTIDGADTLTGISAYER
                     AETVKLFCDENSTSKDFKTPGHIFPLIAKTGGVLVRDGHTEASVDLARLAGFKEIGLI
                     CEIMKDDGTMARVDDLMIFKEKHNLKIITIKDLIEYRKINETTIEKVSSAFLPTKYGN
                     FEIIGYRDTYSNEEHIALTYGNINIENTLVRLHSECLTGDVFHSLKCDCGLQLESSMK
                     KIVENGSGVLIYMKQEGRGIGLLNKIKAYKLQEEGYDTVEANLMLGFEEDMRDFYMAA
                     QILKNLNIKSINLLSNNPDKINQLEKYGIKIENRIPINEEINDFNKLYLKTKKDKMGH
                     LLDII"
     gene            complement(1864..2529)
                     /locus_tag="PC414_003073"
     CDS             complement(1864..2529)
                     /locus_tag="PC414_003073"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HDF2465453.1"
                     /translation="MFTGIVEEVGILRKITANGKSGKVTILSNKILDGTNLGDSIAVN
                     GVCLTVSNLGKNEFTADVMMETIRSTNLGLLNANDKVNLERAMSLSSRFGGHIVTGHV
                     DGKGTICKFEKDENAVLVSIRPDKKLLSSMILKGSVAIDGVSLTISYLDDEIFKVSII
                     PHTKINTILLTKNVGDFVNLESDVIGKYVNNFMANNYKELNSNSNNHKSNIDKDFLFK
                     NGF"
     gene            complement(2563..3675)
                     /gene="ribD"
                     /locus_tag="PC414_003074"
     CDS             complement(2563..3675)
                     /gene="ribD"
                     /locus_tag="PC414_003074"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HDF2465454.1"
                     /translation="MNFVNQKEKDIYYMKKAIELAKNGEGFVNPNPLVGCVIVKASNI
                     IGKGYHEKFGSNHAEVNAINSAKQSLKDSTLYVNLEPCSHYGKTPPCVDKIIQNKIKR
                     VVISTLDPNPLVCGNGVKKLKDNNIDVTVGILEDEARDLNEAFFYHIKNKRPLCIVKS
                     AVSLDGKIATKSLESKWISNESSRYLTHKYRNKYQSIMVGINTVLNDNPLLTCRLNQE
                     KVSHPTRIVIDTHLKLPLNSNLVKDKTSKTIVFTCCKESIKLSMLKENNVETIISPSK
                     NNLVDLEFVMYKLGELNIDSVLVEGGATLNDSLFRNKLVDKVKLFLSPKIIGGKDAPT
                     FVSGEGINHLSDSTQLTINNVTLIDGDILIESDVLN"
     regulatory      complement(3807..3928)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            4117..6600
                     /gene="recQ"
                     /locus_tag="PC414_003075"
     CDS             4117..6600
                     /gene="recQ"
                     /locus_tag="PC414_003075"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HDF2465455.1"
                     /translation="MNKKLLEILSKYYGYTSFRKGQESIINSILSEKDVLAIMPTGGG
                     KSICYQIPALILDGMTIVISPLISLMKDQVDALKTMGISAAFINSSLSSKEFNEILNN
                     IRKNNYKILYIAPERLDAQEFIELINNNNVSQVAIDEAHCVSQWGHDFRLSYRRISDF
                     IKNLPKRPIVTAFTATASEEVRTDIINLLCLENPDYYITGFDRENLSINIVKSSSKNK
                     YILDYIQNHKNESGIIYVATRKEVENIYNGLSKRNISVSKYHAGLSKNERSKNQEDFI
                     NDNVDIMVATNAFGMGIDKPNIRWVIHYNMPQSIENYYQEIGRAGRDGEKSECVLLFS
                     PGDVHIQKYLIDIGVENPERKLFQHKKLQYMIDLVYSNSCYRKTILSYFGENYIDNCN
                     NCSNCLVEGEIVDKTIDAQKVISCVARMKRSYGVTTIVDVLRGSKNKKILQLGLNTLS
                     TYNIMRDYSSEDLKNFVNTLVSHGFLDLVETLGNGNRGSFPTIRLNEMSMKVLKGEVK
                     VEFKEIVMTKSLEVEDELYNALRELRHSIASEERIAPYMVFGDGTLRAMSSSYPINKE
                     EMLDISGVGEIKYQKYGRDFEAVIKEYVEKNNINKKIDEKNVDSKNVNSDFLEVNTDK
                     VLYEKLLVIREEYATKEKLPAYMILANKALKEISGRYPLDEEQLKDISGIGGVKFEKY
                     GASILAEVNKYVKENNINVIWEKKGKRKLILDGESRKNNEIALDLLNQGKDIQSISQE
                     IEVSLSTIIGYVHDYVKDGHSISFELGLENFYNEDKKKMILEVCEKVGYDNLNNIKRK
                     LPDSIKYENIRAVILDSYLENLKNNIIAE"
     regulatory      6689..6800
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6942..8243
                     /gene="thiC"
                     /locus_tag="PC414_003076"
     CDS             6942..8243
                     /gene="thiC"
                     /locus_tag="PC414_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HDF2465456.1"
                     /translation="MNYTTQMDAARKGIITKEMEIVSQKEQVDVNELRELIANGQVVI
                     PANKNHKSLSAEGVGKNLRTKINVNLGISRDCKDIEKELEKVRVAIDMKAEAIMDLSN
                     YGKTREFREKVVEMSPAMIGSVPMYDAVGYLEKELKDITEEEFLNVIRQHAIDGVDFI
                     TIHAGLTRSVCQKIKNHERLTHIVSRGGSLLFAWMELNNKENPIYTNFDKILDICEEY
                     DVTLSLGDACRPGCIKDSTDGVQIQELVVLGELTKRAWERNVQVMIEGPGHMAIDEIE
                     ANVVLEKRLCHGAPFYVLGPLVTDIAPGYDHITSAIGGALACAKGVDFLCYVTPAEHL
                     RLPNLDDMKEGIIAAKIAAHAGDIAKNVKGAREWDNKMSKARADLDWCEMFRLAIDPE
                     KAKRYRDESTPTHEDSCTMCGKMCSMRTVKKILNNEELNLI"
     gene            8297..8491
                     /gene="thiS"
                     /locus_tag="PC414_003077"
     CDS             8297..8491
                     /gene="thiS"
                     /locus_tag="PC414_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HDF2465457.1"
                     /translation="MKVNGKEIEFEKDLTVIDLLNKYNLKSDRVVVEVNLEIIEESNY
                     NTYVLKDEDIVELISFIGGG"
     gene            8484..9287
                     /gene="thiF"
                     /locus_tag="PC414_003078"
     CDS             8484..9287
                     /gene="thiF"
                     /locus_tag="PC414_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HDF2465458.1"
                     /translation="MVKIKIFVNELLTDVEENTTAYKVRDSYNNKCDVLVLNGYPIKT
                     DMTLCENDKLTLIQKGVKPSLDELESLLIARHTPNIHNKLKKGKVAILGLGGLGSNIA
                     ISLARIGVGELLLIDYDVVEPSNLNRQQYFIDDIGKLKTDAMIENIKKINPFIKLNKR
                     DIFLNKNNMDTIKDSDLIIEAFDDASCKAQVCNYVLINLKDKYLIASSGMAGYYDSNI
                     ITTKKIKDRFYICGDFVNEAKFGEGLMAPRVAICANHMANLATQILIDM"
     gene            9350..10120
                     /locus_tag="PC414_003079"
     CDS             9350..10120
                     /locus_tag="PC414_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HDF2465459.1"
                     /translation="MDKLVLGGHEFNSRLLVGTGKYGSNNILPEVIKESGSEIITMAL
                     RRVDLDNKQENILTYIPKEMTILPNTSGATNAEEAVRIARISRKMGCGDFIKIEVISD
                     TRYLLPDNEETIKATKILADEGFIVLPYMTPDLYAGRRLIEANAAAVMPLGAPIGSNR
                     GLQMKEMIRIMIDELDIPIIVDAGIGKPSQAMEAMEMGADAVLVNTAIASAGDPVQMA
                     RAFKLAVEGGREAYIAKTGNVSEFANASSPLTGFLGNL"
     gene            10137..11240
                     /gene="thiH"
                     /locus_tag="PC414_003080"
     CDS             10137..11240
                     /gene="thiH"
                     /locus_tag="PC414_003080"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HDF2465460.1"
                     /translation="MSFYDVIEKYRDFDFDGYLNNVTDNDVLRSLSKDKLEDFDILNL
                     LSKTAVKHLEDMAQKAHKLSVQYFGKTVCLYTPMYIANYCVNQCVYCSYNIKSGIKRK
                     KLTMDEIREEGEVISKEGFKHLLVLTGESSFHSSVEYIGEAIEILREKFPSIGIEVYP
                     MEVEEYKYIVDKGVEGLTVYQETYDEEIYKRVHIKGPKSNYKFRLDAPERGAKAGMRT
                     LSIGALLGLNDFRKETFFTILHGKYLKTKYPHIELSYSTPRMRPFKGCFEELVDISDT
                     DLVQAMVCMRLFDPHAAINISTRENLEMRSHIIPLGVTKLSAGVSTDVGGHSQDEHDT
                     AQFKINDESTVKDVEKMLNSIGYQHVFKDWERF"
     gene            11241..11930
                     /locus_tag="PC414_003081"
     CDS             11241..11930
                     /locus_tag="PC414_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HDF2465461.1"
                     /translation="MYLITNRKLCSEERYLEVIKESILSGVENIIIREKDLEYQELRK
                     LYMKIKTKINCIDFQEQISDESLKTNINQKEFRNKFKVNFIINSNIEFFEKVDCQGIH
                     LPFKLFLNLIENKYNFNENKILGLSLHKVEEVDYLEKLIRNQNIKIDYITLSHIYETK
                     CKEGLNPKGIELLKEAKKITDIKIIALGGILPSNVKETLKYCDDFAIMSTIMRSKDIK
                     KTISNYNEKLN"
     gene            12118..13512
                     /gene="yfcC"
                     /locus_tag="PC414_003082"
     CDS             12118..13512
                     /gene="yfcC"
                     /locus_tag="PC414_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HDF2465462.1"
                     /translation="MTDSKKKRFIIPHTFTIIFLLIVLMAILTWIVPSGEFSREDVEG
                     RMVVVPGTYQSVESNPQGFADVFKAPIEGFIDSAEVVGFVLIVGGAFGIVNRTGAIEA
                     GIASVISKFKGKELLIIPISMILFGLGGTTFGMAEETLPFYMIFVPLMTSLGYDSLTA
                     IAIVFIGAAAGAAASTINPFSVGIAQALAQLAPGSGVTYRVVIFIAYILISIAFVMYY
                     AKKVKENPESSIVYEIDKNNKSILNQTSTEIKEFTFKEVSVLAIFGIGMAIMIWGVLS
                     QGWYIPEISMCFAAIGVLSGLVGRLSQIEISESFIDGAKDLASAALAIALARGIVIIA
                     QNGFIIDTILNSAAAFLGGLPKVIFVYLSFFLEAALAFLIPSSSGLASLTVPVLAPLG
                     DLVNISAQVIVTAYQFGVGLTNFITPTSGVLMGALAVAHIPFSKWVRFIMPILLILTA
                     VSLVFLTIGIYIGF"
BASE COUNT         5124 a         1668 c         2291 g         4627 t
ORIGIN      
        1 ttaaaagttt ttattattaa aaatagctta tttatcgtaa agtgaatgat attgggtttt
       61 aattattatg aatgttagta tttaatttaa agacttaaat aaatttgcca tttctagcgc
      121 attcatagca caatcataac ctttatttcc agcttttgta cctgctcttt caatagcttg
      181 ttcaatattt tctgtagtta cgattccaaa tataacaggt aattcactgt ctaaagatac
      241 tttggctatc cctttagaga cttcactaca tacataatca taatgagaag tagcccctct
      301 tataacacag cctaaacaga ttatagcatc atacttacca cttttagcca ttttttgagc
      361 aacaagagga atctcaaaag ctccaggtac ccaaactatt tctatatttt ctggagaaac
      421 gtcatgtctt aaaagacagt cttcagctcc tgataaaagt tttgatgtta taaattcatt
      481 aaaacgagaa tttattatac ctatttttaa atcttttcca ataagttttc cttcatatat
      541 catttttact tcctccaatt ttatttatgt tatagattta tatttataaa tatctttaat
      601 ttaattataa aaaactactt agtagaaaaa tatcatcata taatcattta tataatatct
      661 aaaagatgtc ccattttatc ttttttagtt tttaaatata gcttattaaa gtcattaatt
      721 tcttcattaa tagggattct attttcgatt tttattccat atttctctaa ttgatttatt
      781 ttatctgggt tattacttaa aagatttata ctttttatgt ttaaattttt taatatttga
      841 gcagccatat agaaatctct catatcttct tcaaatccaa gcattaaatt tgcttcaaca
      901 gtatcgtaac cttcttcttg aagtttataa gccttaattt tatttaaaag accaatacct
      961 ctaccttctt gcttcatata aattaaaact ccacttccat tttccacaat tttcttcata
     1021 gaagattcta gctgtaatcc acaatcacat tttagagagt ggaacacatc tcctgttaaa
     1081 cattcagaat gaagtctaac tagagtattt tctatattta tatttccata agtaagggca
     1141 atgtgttctt catttgaata agtatctcta tatcctatga tttcaaaatt tccatatttt
     1201 gtcggaagga aagcactaga tactttttct atagtagttt catttatttt gcggtattct
     1261 ataaggtctt ttatagtgat gatttttaaa ttatgctttt ctttgaaaat cattaaatca
     1321 tcaactcttg ccattgtacc atcatctttc attatttcac agataagacc aatctcttta
     1381 aaacctgcaa gtctagcaag gtctactgag gcttctgtat gtccatccct gacaagaacc
     1441 ccaccagttt ttgctattaa aggaaagatg tgaccaggcg ttttgaaatc tttacttgta
     1501 gaattttcat cacagaataa ttttacagtt tcagctcttt catatgcaga tataccagtt
     1561 agagtatctg ctccatctat agtaacagta aaggctgttt gaaatgtatc tgtattttct
     1621 ctaaccataa gaggtatatt aagactttta aatttttttt cagtggcagg tagacaaatt
     1681 agacctctac catatgtagc cataaaattt attgattcag gagtagccat ttcagctgcc
     1741 atcaataaat ctccttcatt ctctctactt tcatcatcaa ccacgattac cattttccca
     1801 ttttttatat cttcaatagc ttcttcaata gtattaaaca tgtaattcac tcctttatta
     1861 tttttaaaaa ccatttttaa ataaaaaatc tttatctata tttgatttat ggttattact
     1921 attagaattt aattccttgt agttattagc cataaaatta ttaacatact ttccaattac
     1981 atcgctttct aaattaacga aatctccaac attctttgtc aagagaatag tgttaatttt
     2041 agtatgaggt ataatagaaa ccttaaatat ttcatcatct aaatatgata tagttaaact
     2101 tacaccatca atagcaacag aacctttgag tatcattgag gataaaagtt ttttatcagg
     2161 tcgtatactt actaaaactg cattttcatc tttttcaaat ttgcagatag ttcctttacc
     2221 atcaacatga cctgttacta tatgaccacc aaatcttgaa gaaagactca tagctctttc
     2281 aagatttacc ttgtcatttg catttaataa acctaaattt gtggaacgaa ttgtctccat
     2341 cataacatcg gctgtaaatt cattttttcc gaggtttgaa acagttagac aaactccatt
     2401 tacggctata ctatctccaa gatttgtgcc atctaatatt ttatttgata gtatggttac
     2461 ttttccactc ttaccattag cagtaatttt tctaagtatt ccaacttctt ctactatgcc
     2521 agtaaacatc ctaacctcct atatgaatgt attaacaaaa atttaattta aaacatctga
     2581 ttctataaga atatcgccat ctatcaaagt tacattattg attgttaact gtgttgaatc
     2641 tgacaaatga tttattcctt ctccagaaac gaatgtaggt gcatctttac cacctataat
     2701 cttgggtgat agaaataatt tgaccttatc aactaactta tttctaaaga gactgtcatt
     2761 gagagtagca ccaccttcaa ctagaacaga atcaatgttt aactctccta atttatacat
     2821 tacaaactcc aaatctacta agttgttttt tgaaggagaa attattgttt caacattatt
     2881 ttcttttaac atagataatt ttatactttc tttgcaacat gtgaaaacta tagttttact
     2941 ggttttatct ttaaccaaat tactattaag tggcaacttt aggtgtgtat ctattactat
     3001 tcttgtagga tgagacactt tttcttgatt gagcctacaa gttagtaaag gattatcgtt
     3061 caaaactgta tttatgccaa ccattatact ttggtattta tttctgtatt tatgagttag
     3121 ataccttgat gattcattag atatccattt tgattctagt gattttgtag caattttacc
     3181 atctagactt actgctgatt ttacaataca aagaggtctt ttgtttttta tatgataaaa
     3241 aaatgcctca tttaaatctc ttgcttcatc ttctaatatt cccacagtaa catcaatatt
     3301 gttatctttt aattttttta ctccattacc acaaacaagt ggatttgggt ctaatgtaga
     3361 tattacaact ctcttaatct tattttgtat aattttatct acacaaggag gcgttttccc
     3421 ataatgagaa catggttcta agtttacata aagagtagag tcttttagac tttgtttagc
     3481 actgtttata gcatttactt cagcatgatt agagccaaat ttctcatgat atcctttgcc
     3541 tattatatta ctagctttaa caattacaca cccaactaat ggatttggat ttacaaagcc
     3601 ttctccattt ttagccaatt caatagcttt tttcatataa taaatatctt tttctttttg
     3661 attcacaaaa ttcacaatat gtacacctcc tgtttaattg gataattttt aaacaaaaaa
     3721 ccctgagaat taactcaggg tttagaataa tctacaataa atgtttaata aatgtagaac
     3781 aaaattgctc tacaaaaaca ctcattaaat atcttcttcc atccagactt tactgtcggc
     3841 tttggaattt aaccaaatca accaagtttt accttggctc gcgggctata ccgccgattg
     3901 ggaatttcac cctaccccga agattaagta ttaaatttat atttagaatg tatcataaat
     3961 tattttactt tgcaaactat ttttttaaaa aatatatgtt aatatataat atagaatgtc
     4021 tataaacttt gaaataccac gattgtgaat ataaaaataa aaatttgaac ataatcagga
     4081 aagaagtcta attaatttaa attaggagag taatatatga ataaaaaact tttagaaata
     4141 ctatcaaaat actatggata tacaagtttt agaaaaggtc aagaatctat aataaactct
     4201 atcttaagcg aaaaagatgt gttagctatt atgccaacag gagggggaaa atcaatatgt
     4261 tatcaaattc cagctttgat tttagatgga atgacaatag taatttctcc acttatatca
     4321 cttatgaaag accaagtaga tgccttaaag actatgggaa ttagtgctgc ttttataaat
     4381 agttcattaa gtagcaaaga atttaatgaa atattaaata atattagaaa aaataattat
     4441 aaaatactct atattgctcc agagagatta gatgcacaag agtttattga gctaataaat
     4501 aataacaatg taagtcaagt tgcgatagat gaagcacatt gtgtatctca atgggggcat
     4561 gattttaggt tgagttatag aaggatttct gattttataa aaaatttgcc taaaagacct
     4621 atagttacag catttactgc aacagcttca gaagaggtaa gaactgatat aataaattta
     4681 ctgtgtttag aaaatccaga ttattatata actggatttg atagagagaa tttatcaata
     4741 aatatagtaa aatcatctag taaaaataag tatattttag attatattca aaatcataaa
     4801 aatgagtctg gaattatata tgttgcaact agaaaggaag ttgaaaatat atataatggt
     4861 ttatctaaga gaaatatatc tgtgtcaaaa tatcatgcag gtttatctaa aaatgaaaga
     4921 agtaaaaatc aagaagattt tataaatgat aatgtggata taatggtagc aacaaatgca
     4981 tttggaatgg gtattgataa acctaatata agatgggtta tacattacaa tatgcctcaa
     5041 agtatagaaa actactatca agaaataggt agagctggta gggatggaga aaaaagtgag
     5101 tgtgtacttt tattttctcc aggagatgta catatacaaa aatatttaat tgatattgga
     5161 gttgaaaatc ctgaaagaaa attatttcag cataagaagc ttcaatacat gattgactta
     5221 gtttacagta attcatgcta tagaaagaca atattgagtt attttggaga aaattatata
     5281 gacaattgca ataattgtag caattgttta gtagagggtg aaatagtaga taaaactata
     5341 gatgcacaaa aagtcatttc atgtgtagct agaatgaaaa gaagttatgg tgttactact
     5401 attgtagatg tgcttagagg ttctaagaat aaaaagattt tacagcttgg attaaatact
     5461 ttgtctacat ataacattat gagagattat tctagtgaag acttaaagaa ttttgtaaat
     5521 acattggttt ctcatggttt tttagattta gttgagactc taggcaatgg aaatagaggt
     5581 agttttccta ctataagatt aaatgagatg tctatgaaag ttttaaaagg tgaagtgaaa
     5641 gtagagttta aagaaattgt tatgacaaaa tcattggaag tagaggatga gttatataat
     5701 gctttaagag aacttagaca ttcaattgca agtgaagaaa ggatagctcc atatatggta
     5761 tttggagatg gtacacttag agccatgtct agtagttatc caattaataa agaagaaatg
     5821 ctagatatct ctggagtagg agaaattaaa tatcaaaaat atggtagaga ttttgaagca
     5881 gtaattaaag aatatgtcga aaaaaataat ataaataaaa aaatagatga aaaaaatgtc
     5941 gattcaaaga acgttaatag tgatttctta gaggttaata cagataaagt tttatatgaa
     6001 aaactgttag tcattagaga agaatatgca acaaaggaaa aattacctgc atatatgatt
     6061 ttagccaata aggctttgaa agaaataagt ggaagatatc cacttgatga agaacaatta
     6121 aaagatattt ctggcatagg tggagtaaaa tttgaaaaat atggagctag tattttggca
     6181 gaggttaata aatatgtaaa agaaaataat attaatgtta tttgggagaa aaagggcaaa
     6241 aggaagttaa tattagatgg agaaagtaga aaaaataatg aaatagcatt agatttgcta
     6301 aatcaaggaa aagatataca aagtatcagt caagaaatag aagtttcact gtcaaccatt
     6361 attggatatg tgcatgacta tgtaaaggat ggacatagta tatcatttga attaggacta
     6421 gaaaattttt ataatgaaga taaaaagaag atgattttag aagtttgcga aaaagttggc
     6481 tatgataatt taaataacat aaaaagaaaa cttccagatt caataaagta tgaaaatata
     6541 agagctgtga tacttgactc ttatcttgaa aacttaaaaa ataatattat agcagagtaa
     6601 tttttaactt aataaaaaat aaaatttgta ttattgtgtt gaactaataa ttttgctgtg
     6661 ttatactgaa tttgtaaaaa taatgaaaaa ttagagctag gggcgctaga attggctagc
     6721 tgagagataa agccgtaact tacaaaccct aataacctga tcaagataat gcttgcgaag
     6781 ggaagctttt taatataatt ttattatata aataggcaaa ttattcaatg ttaggagaaa
     6841 tttgtctaag tgtatatacg attataagag tgctttccaa acgaggaaaa gcactttttt
     6901 tattttcaaa aactataact ttaatcttgg aggattgtaa tatgaactat acaacacaaa
     6961 tggatgctgc tagaaaaggc ataataacta aagaaatgga aatcgtttct caaaaagaac
     7021 aggtagatgt aaatgaatta agagaattaa tagcaaatgg acaagttgtt ataccagcta
     7081 ataaaaatca taaatcttta agtgcagaag gtgtaggtaa aaatttaaga acaaaaataa
     7141 atgtaaatct aggaatttca agagattgta aggatataga aaaagaactt gaaaaagtta
     7201 gagtagcaat agacatgaaa gctgaagcta taatggattt aagtaattat ggcaaaacta
     7261 gagaatttag agaaaaggtt gttgaaatgt cacctgcaat gataggttca gtacctatgt
     7321 atgatgcagt aggatatttg gaaaaagaat taaaagatat aacagaagaa gagtttttaa
     7381 atgtaataag acaacatgct attgatggag tagactttat tactatacat gcaggtctta
     7441 caagaagtgt ttgccaaaaa ataaaaaatc atgagagatt gactcatata gtatctcgtg
     7501 gaggttcttt attgtttgca tggatggaat taaataataa ggaaaatcct atttatacta
     7561 attttgataa aatattggat atatgtgaag aatatgatgt tacattgagt ttaggagatg
     7621 cttgtagacc aggttgtata aaagattcta ctgatggtgt tcaaattcaa gaacttgtag
     7681 tattaggtga acttactaaa agagcatggg aaagaaatgt acaagtaatg atagaaggtc
     7741 ctggacacat ggctattgat gaaatagaag caaatgtagt attagaaaag agattatgcc
     7801 atggagcacc tttttatgtt cttggaccat tggttacaga tatagcacca ggttatgacc
     7861 atataacaag tgcaatagga ggagctttag catgtgccaa aggagtagat tttttatgtt
     7921 atgtaactcc agcagaacat ttaagattgc ctaatttaga tgatatgaaa gagggtataa
     7981 tagctgcaaa gatagctgct catgcaggag atatagctaa aaatgttaaa ggtgctcgtg
     8041 aatgggataa taagatgagt aaagctagag ctgatttaga ctggtgtgag atgtttagac
     8101 ttgcaataga ccctgaaaaa gctaagagat acagagatga atcaactcct actcatgagg
     8161 atagttgtac tatgtgtgga aaaatgtgct caatgagaac agttaagaaa atattaaaca
     8221 atgaggaact taatctaata taatttttgg ctagtatgac tggaataaaa ataagctgaa
     8281 aagaggaggt gatttagtga aggttaatgg gaaggaaatt gaatttgaaa aagatttgac
     8341 tgttatagat ttattaaata aatataattt aaaaagtgat agagttgtag ttgaagtgaa
     8401 tttagagata attgaagaat ctaactacaa tacatatgta ttaaaagacg aggacattgt
     8461 agagcttatt agctttattg gaggtggtta aaataaaaat atttgtaaat gaattattaa
     8521 cagatgttga ggaaaataca actgcttata aagttagaga tagctataac aataaatgtg
     8581 atgtattggt gttaaatgga tatccaatta aaacagatat gaccctttgt gaaaatgata
     8641 aattaacact tatacaaaaa ggagttaagc catcacttga tgaattggaa agcttgttga
     8701 ttgctagaca tactccaaat atacataata aattaaaaaa agggaaagtt gctatattgg
     8761 gattgggggg acttggttcc aatatagcta tctctttagc tagaatagga gtgggagaac
     8821 ttttacttat agattatgat gttgtagaac catctaattt aaatagacag cagtatttta
     8881 tagatgatat aggaaaacta aaaactgatg ccatgataga aaatataaaa aaaataaatc
     8941 catttataaa attaaataaa cgagatattt ttttaaacaa gaacaatatg gatactatta
     9001 aggattcaga tttaataatt gaggcctttg atgatgcttc atgtaaagca caggtatgta
     9061 attatgtatt aataaattta aaagataaat atttaatagc atcatctggt atggcaggtt
     9121 actatgattc aaatataata actactaaga agataaaaga caggttttat atatgtgggg
     9181 attttgtgaa tgaagctaaa tttggtgaag ggcttatggc tccaagggtt gccatctgcg
     9241 caaatcatat ggcaaatttg gcaacgcaaa tacttataga tatgtaaata ttaaaattag
     9301 atataaaaaa gattgaatat taaaaaaatt taacgtggag ggaaataata tggataaatt
     9361 ggtacttggt ggacatgaat ttaatagcag attattggtt ggaacaggta aatatggttc
     9421 aaataatata ttgcctgagg taataaaaga aagtggaagt gaaataataa caatggcact
     9481 tagaagagtg gatttagaca ataaacaaga aaacatatta acgtatattc caaaagagat
     9541 gacaatactt ccaaatacat caggagctac taatgcagaa gaagctgtaa gaatagctag
     9601 aatatctaga aaaatgggat gtggagattt tataaaaata gaagtaatat cagatactag
     9661 atatttactt cctgataatg aagaaactat aaaagctaca aagatacttg ctgatgaggg
     9721 gtttatagtt cttccatata tgacaccaga cctttatgct ggaagaagac ttatagaagc
     9781 taatgcagcg gcagttatgc cacttggagc tccaattggt tcaaatagag gtcttcaaat
     9841 gaaagaaatg ataagaataa tgatagatga gttggatata ccaataatag tagatgcagg
     9901 tattggaaaa ccatctcaag ctatggaggc tatggaaatg ggagctgatg cagtacttgt
     9961 caacactgct atagcttcag caggagaccc tgtacaaatg gctagagcat ttaaattagc
    10021 tgtagaaggt ggaagagaag catatattgc aaaaacagga aatgtgtcag aatttgcaaa
    10081 tgcatcttct cctttaacag gttttttagg caatttatag aaagtggtga ttggtgatga
    10141 gtttttatga tgtaatagaa aaatataggg attttgattt tgatggatat ttaaataatg
    10201 ttactgataa tgatgtttta agaagtttat caaaagacaa gcttgaagat tttgatatat
    10261 taaatttgct ttctaaaact gcagtaaaac atttagaaga tatggcacaa aaagcacata
    10321 aattaagtgt tcaatacttt ggtaaaacag tatgtttata tacaccaatg tatatagcaa
    10381 attattgtgt taatcaatgt gtttattgta gctataatat aaagagtgga ataaaaagaa
    10441 aaaaattaac tatggatgaa attagagaag agggagaagt aatttcaaaa gaaggtttca
    10501 aacatctact tgttttgact ggagaaagta gttttcactc aagtgtagaa tatataggtg
    10561 aggctataga aatattaaga gagaaatttc cctctatagg tatagaagtt tatccaatgg
    10621 aagtagaaga atataagtac attgtagata aaggtgttga aggattaaca gtatatcaag
    10681 aaacttatga cgaggaaata tataagagag tacatataaa aggcccaaaa tcaaattata
    10741 aatttagact agatgctcct gaaagagggg caaaagcagg tatgaggact ttatcaatag
    10801 gtgcactttt agggctaaat gattttagaa aagaaacttt ttttacaata ttgcatggga
    10861 aatacttaaa aacgaaatat cctcatattg agctttcata ttcaacacca agaatgagac
    10921 cttttaaagg gtgttttgag gaattggtag atataagtga tactgattta gttcaagcta
    10981 tggtatgtat gagattattt gaccctcatg cagctataaa tatatccact agagaaaatt
    11041 tagaaatgag aagtcatata atacctttag gagtaactaa attgagtgca ggtgtatcta
    11101 ctgatgtagg aggtcattct caagatgaac atgatactgc tcaatttaag ataaatgatg
    11161 aaagtactgt taaagatgtg gagaaaatgc taaactcaat tggttatcag catgtgttta
    11221 aagattggga aagattttaa atgtatctga taacaaacag aaaattatgt agtgaggaac
    11281 gatatttaga agttattaaa gagtctatat tgagtggtgt agaaaatata ataataagag
    11341 aaaaggattt agaatatcaa gagctaagaa aattatatat gaaaataaaa actaaaatca
    11401 attgtataga ttttcaagag caaatatcag atgaaagttt gaaaactaat ataaatcaaa
    11461 aagagtttag aaataaattc aaggttaatt ttataataaa tagtaacatt gaattttttg
    11521 aaaaagtgga ttgtcaaggt atccatcttc cttttaaatt attcttgaat ttaattgaaa
    11581 ataagtataa ttttaatgaa aataaaatac taggattatc tttacataaa gtagaggaag
    11641 tagattattt agaaaaatta ataagaaatc aaaatataaa aatagattac ataacattat
    11701 cacatatata tgaaactaaa tgtaaagaag gattaaatcc aaaaggaatt gagcttttaa
    11761 aagaagctaa aaagattact gatattaaaa taattgcact tgggggaatt ttaccatcta
    11821 atgtcaagga aacattaaaa tactgtgatg attttgctat aatgtcaact ataatgaggt
    11881 ctaaagatat taaaaaaacc atatcaaatt ataatgaaaa gttgaattag taaatatagt
    11941 atattgaatt tttaacttaa ataaaaataa aaaagttatc ttttaaattt taaaaggtag
    12001 cttttttatt ttatatcttt gctaataaaa aattgtaaaa agggaaaact ttagtcagag
    12061 agattaaaca atatcctaga aagatttaaa atttagtaaa gtatggagga cattataatg
    12121 actgacagta aaaagaaacg atttattata ccacacactt ttacaataat atttttacta
    12181 atagtcttga tggcaatatt gacttggata gtaccaagtg gtgaatttag tagagaagat
    12241 gtagaaggta gaatggtagt tgtaccaggg acttatcaaa gtgtagaaag taatcctcaa
    12301 ggatttgcag atgtttttaa agcaccaata gaaggcttta tcgattcagc tgaagttgta
    12361 ggttttgttt taatagttgg aggtgctttt ggtattgtaa ataggactgg agctatagaa
    12421 gcaggaatag cttcggttat ctctaagttt aagggaaaag aacttcttat aattccaata
    12481 tcaatgattt tatttggact tggaggtaca acatttggga tggcagaaga gacattacca
    12541 ttttatatga tatttgttcc actcatgaca tccttgggat atgattctct aacagcaata
    12601 gcaattgtat ttataggagc agcagcagga gcagccgctt caacaataaa tccattttca
    12661 gttggtattg cacaggcact agctcaactt gctcctggtt caggagtaac ttacagagta
    12721 gttattttta tagcatatat actaataagt atagcatttg ttatgtacta tgctaagaag
    12781 gtaaaggaaa atcctgaaag ttcaatagta tatgaaatag ataaaaataa taaatcaata
    12841 cttaatcaaa cttcaacaga aataaaagaa tttactttta aagaagtatc tgttcttgct
    12901 atatttggaa tagggatggc aataatgatt tggggtgttt taagtcaagg atggtacatt
    12961 ccagaaatat caatgtgttt tgctgcaata ggtgtgcttt ctggattggt tggtagatta
    13021 tctcaaattg aaatctcaga aagttttata gatggagcaa aagatttagc atctgcagct
    13081 cttgctatag ctttggctcg tggaatcgta ataattgctc aaaatggatt tataatagat
    13141 actatattaa attccgcagc agcctttcta ggtgggcttc caaaggttat atttgtatat
    13201 ttatcatttt tcttggaagc tgcattggca tttttaattc catcatcttc aggtcttgct
    13261 tcacttacag tacctgtact tgctcctctt ggtgatttgg ttaatatatc ggctcaagtg
    13321 atagtaaccg cttatcaatt tggtgtgggt cttacaaact ttataactcc aacttcagga
    13381 gtactgatgg gagcacttgc agtagcccat attccatttt caaaatgggt aagatttata
    13441 atgcctatct tgttgatttt gacagctgta tctctagtct ttttaacaat aggtatttat
    13501 atagggtttt aattaagtgt agttttattt aagatattta gtaatggtat aaaatgataa
    13561 tatgttttca ataaattgcc actaatttaa ttagtggtaa tttgtttttt gtatttaaaa
    13621 ataaatgtat ttacttgtat tttgaattaa tatctactta gacattgttt taattgttat
    13681 atttttattt aattaagcat tgatttatca
//
LOCUS       DAMJVK010000101        13401 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.105, whole genome shotgun sequence.
ACCESSION   DAMJVK010000101 DAMJVK010000000
VERSION     DAMJVK010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13401
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.105"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            179..2038
                     /locus_tag="PC414_003083"
     CDS             179..2038
                     /locus_tag="PC414_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2465463.1"
                     /translation="MKRFLRRIILVLFILLIFISFISIKLIHNVGDYGKLINYVGIVR
                     GASQRLTKLEMNHQPNDELIEYIDEILQELITGHGDYGLVITDCNEYNEDLLLLEKKW
                     EDLNIEIKKVRMKEQNNQLLSISEEFFSLANDTVFKIENFSKEKSNYLMTLIIIISII
                     GILACIILILQYSKKMVRLEKLNVDLKNIAYKDELTGVNTIEKFKLDANQNICIHKDK
                     KFAVFYIDFENFKYINDIFGYDYGDMILKRYANLMMNDIGKYEIFAREIADRFVALRC
                     YVDKEDLVVRQRDVDNELINITNEIKNKHSITVVSGICCIEDVNEKLSIDGLINRANF
                     AQKTVKNKPGTNYAFYNDSIRKKMIEENTIKSRIHEAIEKREFIVYLQPKVNLHSQKI
                     NCAEALVRWLTPDKGIISPAIFIPVLEKNFFIALVDKYVFEEVCKWIRKRLDENKPFV
                     QISVNVSRIQFYNTKFVETYSNIQNKYRIPKNTIEIEFTESVAFENQKHLLEIIHDLH
                     ENGFTCSLDDFGKGYSSLSVLKDLPFDVLKLDSMFFKASLDKDKEKIVIKNIVHMLKE
                     LNITTVAEGIECKEQVEFLRDIGCDLVQGFVFYKPMPILEFEKILDKEFVYNS"
     gene            complement(2330..2929)
                     /locus_tag="PC414_003084"
     CDS             complement(2330..2929)
                     /locus_tag="PC414_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF2465464.1"
                     /translation="MAIELKKGQKINLTKKENSDLGEILVNLNWNQKVQKKGFFGSLR
                     SSNIDLDLGCLFEMKNGVKGAVQALGNAFGSLDNPPFAQLDGDDRTGSNTQGENLRIN
                     GNKIKDIKRILIYAFIYEGVANWSEADGIVTIKQKQDSDLVVKLDEHKNGYNMCSIAL
                     IENVNDETFSVEKVVKYFKGHREMDTEFHWGLKWVAGRK"
     gene            complement(3032..3829)
                     /locus_tag="PC414_003085"
     CDS             complement(3032..3829)
                     /locus_tag="PC414_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDF2465465.1"
                     /translation="MKFKKLLCLLLCLVLTLAVVGCSKAKDDKKIVVGATLVPGGELL
                     EELKPLIKEKGYTLEVKNFDDYILPNEALNNGEIDANLFQHEPYLKEAVKAKGYKIMA
                     GKKLYVCPAILYSYKIKSVDEFKKGDTIAISNNPSSCSKNLRYLESIGLLTLPKGDGL
                     VSPKDIIENPKGIQFKELDIAQIPSSLPDVTAAFIDTTYAVPAGLDAKKNGIYTAPIN
                     DEYANLLAFRTEDKDSEKIKVLQDVLTSDKARSLIEEKYKGIVIPTF"
     regulatory      complement(3942..4047)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(4139..5125)
                     /locus_tag="PC414_003086"
     CDS             complement(4139..5125)
                     /locus_tag="PC414_003086"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HDF2465466.1"
                     /translation="MLLIYNEKTNPYFNLAMEEYLLKNFDEDLFILWRNEPSIIVGKN
                     QNTLSEINLEYIKEYSIPVVRRQSGGGAVFHDLGNINFTFIACNNNNFSDFRRFTQPI
                     IDLLKTLDVNAEFSGRNDLLIDGKKFSGNAQYNYKNKVMHHGTLLFSSEINDLSKALK
                     VKPIKFEGKGIKSVKSRVTNISEHLHSKMDILEFKDAIMDYLSKTNTDNTNYCLSEHD
                     IKQIEKLTKSKYETWDWNFGNSPKYTLSNELKYSGGNVEFNLNVDKGIITSIKFFGDF
                     FGKCDVAFVENKLIGIRHEENALRNTLDSIEINDYFLGADTDILVSGILGVK"
     gene            5609..7339
                     /locus_tag="PC414_003087"
     CDS             5609..7339
                     /locus_tag="PC414_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HDF2465467.1"
                     /translation="MRNKKINIIFLTTIMFIMSTVMVFAEEDIDTIALANAEKFGILT
                     LIPPLVAIILAFITKNVIISLLIGILSGSFIIKASGINVFATFIQAFLDLVDRALVSL
                     ADPWNAGIILQVLSIGGVINLVAKMGGAKAIAEALAKRAKSAKGTQLITWFLGLLVFF
                     DDYANSLIVGPMMRPVADKMKISREKLAFIIDATAAPVAGLAIISTWIGLEVGLIHDA
                     FESISIDVDAFGIFLNTIPFRFYNILILAFIVISALLLKEFGPMRKAEIKSRSRKISI
                     DLDEGVEELDDLAPKNGVKLSVWNAIIPIGTLIIVALASFYYSGYTSIMGGDDKALIQ
                     LFTNSPYSFEAIKEAFSASDASRALFQSALVASLVAIIMAVVKKIFTISEAIDVWIDG
                     MKSLVITGVILILAWSLSSVIKELGTAKFLIHLLSGSLPPFLLPSLIFGLGAIISFAT
                     GTAYGTMGILMPLAIPLAYSLNPDMSYVIVSTSAVLTGAIFGDHCSPISDTTILSSMG
                     AGCNHIDHVNTQMPYAIFTAVITIVFGYIPAGLGLPIYIVLPVAIAAIFVGIQIIGKK
                     VDEAEIELVE"
     gene            7449..7529
                     /locus_tag="PC414_003088"
     tRNA            7449..7529
                     /locus_tag="PC414_003088"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7483..7485,aa:Leu,seq:tag)
     gene            7864..8379
                     /locus_tag="PC414_003089"
     CDS             7864..8379
                     /locus_tag="PC414_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HDF2465468.1"
                     /translation="MEKYIWLFPLLFIFHDMEEIIGFGIWLKKNKSMLDKKYPFISKV
                     YENYSTEGMAFAVFEEFILCIIFCILTVITENQYVYLLWLGSFIAYTLHLVIHIGQSI
                     IIRKYIPSLITSIICLPISIWCISKSIYIVDCEMSTTILYSIIGIIIVALNLKFAQSL
                     IGKFTKWMSNI"
     regulatory      8871..8975
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            9185..11659
                     /gene="gcvPA"
                     /locus_tag="PC414_003090"
     CDS             9185..11659
                     /gene="gcvPA"
                     /locus_tag="PC414_003090"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HDF2465469.1"
                     /translation="MNELKRVSLYNIHKELGAKLVEFAGWEMPLEYEGINKEHEKVRK
                     SAGIFDVSHMGEVQIKGAESEKFIQNLVTNDISALKINDIIYTPMCYENGGVVDDLLI
                     YKFGEEDYLLVINAGNIDKDVAWIIKQSEGYNVDIKNISSEVSQLAIQGPKAEEILQK
                     ITDIDLNSIKFYKSIPSTKVCGCPCLVSRTGYTGEDGFEIYCKNKYVEIIWNEVLKVG
                     GEDICPAGLGCRDTLRFEAALPLYGHEINEHISPIEGGLSIFVKTNKESFIGKSILSK
                     EKESGAKRKLVGFEMQGKGMPRNGYDIRIGDKTVGFVTTGCASPTTGKILGMGIIDSE
                     YAKVGNEIGIAIRKKVVPAVIVKKPFYKKQYKKDNIILNKENKFSYIPATSEDKSKML
                     KVVGLNSVDELFSDIPEEVKLKRDLNLEIGKSELEVSKIVKRLSEENLSLEDLTCFLG
                     AGAYDHYIPSIIKHITSRSEFYTAYTPYQAEISQGTLQVVFEFQSMIAEITGMEIANA
                     SMYDGATAAIEACIMAMNQTRKSKIVVSKTIHPETLSVLRTYLQYKDCEIVEIDFCNE
                     YGTTDIEKLKASVDKDTACVLIQTPNFFGIIEEMEEIEKITHENKAMLIMSVDPISLG
                     VLKTPGEIGADIVVGEAQSLGNPLNFGGPYVGFLASKSKYTRKMPGRIVGQSLDVEGK
                     IAYVLTLQTREQHVRREKATSNICSNQALNALVASIYMATMGKEGFKEVGMQSMKKAH
                     YTYNKLVQTGKYKPIFKGKFFKEFAVQGNLNIETINDKLLEENILGGYNLEYNYPELK
                     NSTLLCVTEKRSKEEIDKLVGIMEGL"
     gene            11659..13116
                     /gene="gcvPB"
                     /locus_tag="PC414_003091"
     CDS             11659..13116
                     /gene="gcvPB"
                     /locus_tag="PC414_003091"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HDF2465470.1"
                     /translation="MKEYNSLLIDISKKGRKAYSLPKLDIDDIKIENMIDQDMARQSE
                     LNLPEVGELELVRHYTLLSNKNFGVDTGFYPLGSCTMKYNPKINEDMAALPNFTGMHP
                     YQSSDTAQGSLSLMYDLSRRLAEITGMDEVTLQPSAGSHGEFTGLMIIKAYHENRGDH
                     KRTKVIIPDSAHGTNPASAAMANFDVIQIASDKNGAVDINALKEVLNDEVAALMLTNP
                     STLGLFEKNIKEIATLVHEAGGLLYYDGANMNAIMGITRPGDMGFDVVHLNLHKTFST
                     PHGGGGPGAGPVGVKKELVPFLPIPVIERKEDKYVLNYDREKSIGKIKNFYGNFGVLV
                     RAYTYILTMGRDGLKEASEMAVLNANYIKESIKDDYILPIDTLCKHEFVLGGLPRGEA
                     NIKTIDIAKRLLDYGYHPPTMYFPLIINEALMIEPTESESIETLDSFIEAMKSVAKEA
                     KEEPELLKTAPHNTLVKRVDDARAVKKPILTWSQR"
BASE COUNT         5032 a         1589 c         2203 g         4577 t
ORIGIN      
        1 tatattagaa aaaaattttt gtatatataa aggactctaa agatgaacta tgtaaaacaa
       61 ggtttatata ttctaattgt atagaaaaat ttattgtaaa cgcatgtata ttattagaaa
      121 aaatagtttg gaggtattca ataaacataa aaaacaatat aggggataaa ggtgttttat
      181 gaagagattt ttgaggagaa ttatcttagt tttatttatt ttgttgattt ttataagttt
      241 tatttctatt aaattaattc ataatgttgg agattatgga aaattgatta attatgttgg
      301 gattgttcgt ggagctagcc aacgtctaac taaactagag atgaatcatc aaccaaatga
      361 tgaattaata gaatatattg atgaaatcct acaggagttg attacagggc atggagatta
      421 tggtttagta atcactgatt gtaatgaata caatgaggac ttacttttat tagaaaaaaa
      481 gtgggaagat ttaaatattg aaattaaaaa agtaagaatg aaagaacaga ataaccagtt
      541 attaagtata agtgaagagt ttttttctct tgcaaatgat acagtgttta aaatagaaaa
      601 cttttctaaa gaaaaaagta attacttaat gactttaata ataattatat ctattatagg
      661 tatattagca tgtatcatat tgattttaca atatagtaaa aaaatggtta gactggaaaa
      721 attaaatgta gatttaaaaa atattgcata taaagatgaa ctaacaggag taaatactat
      781 agaaaaattt aaattggatg caaatcaaaa tatttgtata cataaggata agaaatttgc
      841 tgtattctat attgattttg aaaactttaa atacattaat gatatatttg gatatgatta
      901 tggtgacatg atactgaaaa gatatgctaa tcttatgatg aatgacatag ggaaatatga
      961 aatatttgca agagaaattg cagatagatt tgttgcgtta agatgctatg tagataagga
     1021 agatttagtg gtaagacaac gagatgtaga caatgaacta attaatataa caaatgaaat
     1081 taaaaataaa catagtatta ctgttgttag tggaatttgt tgtattgaag atgtaaatga
     1141 aaaattaagt atagatggtt taataaatag agcaaatttt gcacaaaaaa ctgttaagaa
     1201 taaaccaggt actaattatg ctttttataa tgacagtatt cgtaaaaaaa tgattgaaga
     1261 aaatactatc aaaagtagaa tacatgaagc tattgaaaaa agagaattta tagtttattt
     1321 acagcctaaa gttaatttac atagtcaaaa aataaattgt gcagaagctt tagttcgttg
     1381 gcttactccg gataaaggta ttatatctcc agcaatattt attccagtac ttgagaagaa
     1441 tttttttatt gccttagtag ataagtatgt ttttgaggag gtttgtaaat ggattagaaa
     1501 gaggttagat gaaaataaac cttttgttca gatatcagtt aatgtttcaa gaatacaatt
     1561 ttataacact aaatttgtag aaacttattc caatatacaa aataaataca ggatacctaa
     1621 aaatactata gaaattgaat ttacagagag tgttgctttt gagaatcaaa aacatctttt
     1681 ggaaattata catgatttgc atgaaaatgg atttacttgt tctttagatg attttggaaa
     1741 aggttattcg tctttaagtg ttttaaaaga tttaccattt gatgttttaa agcttgattc
     1801 tatgtttttc aaggctagtt tagataaaga taaagagaaa atagtaataa aaaatattgt
     1861 acatatgtta aaagaactta atatcacaac tgttgctgag ggcattgaat gcaaggaaca
     1921 agttgaattt cttagggata ttgggtgtga tttagttcag ggatttgtat tttataaacc
     1981 aatgccaata ttagaatttg aaaaaatatt ggataaagag tttgtttata atagctaaaa
     2041 tgtactttat aaaatatgaa ctaattaagt aaaattattt tgtactgaat aaatttttac
     2101 tttatttaca gaataatgat tatttacaga aaagcgcgaa aagtgtattc ctttaagtgt
     2161 gggatggata gcacaaatag aggagaaaaa atattggcca tataagaatg cataataata
     2221 taaatattta aaaggtcagc attatataaa aattaaaaag aaaataaata atttttctat
     2281 aaaaaagaga ctatgagaaa atttactcat agtctcctaa ataatgtatt tattttcttc
     2341 ctgcaaccca ttttaatccc caatgaaatt cagtatccat ttctctatga cctttgaagt
     2401 atttaactac tttttcaaca ctgaatgtct catcatttac attttctatc aatgctattg
     2461 aacacatatt atagccattt ttatgttcat caagtttgac aactaagtct gaatcttgtt
     2521 tttgtttgat tgttacaatt ccatcagctt cagaccagtt agctacacct tcgtatataa
     2581 aagcataaat aagtattctt tttatatctt ttattttatt accatttatt cttaaatttt
     2641 ctccttgtgt attacttcct gttctgtcat ctccatctaa ttgagcaaaa ggtggattgt
     2701 ctaaactacc aaaggcattt cctaatgctt gaacagcacc ttttacacca tttttcatct
     2761 caaataggca acccaaatct aaatctatat tactgcttct taaagagcca aaaaaaccct
     2821 ttttttgtac tttttgattc caatttaaat taactaaaat ttcacctaaa tcgctatttt
     2881 ccttttttgt aaggtttatt ttttgtccct tttttaattc tatagccata gtaatcttcc
     2941 tccacaaaca tagatttatt taaatggtag cacttaataa gtcaatattc aacaagatta
     3001 gaataataat attaaaatta tactaaatat actaaaaagt aggaatcaca atacctttgt
     3061 atttttcttc aattaagctt cttgctttat ctgatgtaag aacatcttgt agaactttaa
     3121 tcttttcact gtctttatct tcagttctaa atgctaaaag atttgcatac tcgtcattta
     3181 taggagctgt ataaattccg tttttcttgg catcaagacc agcaggtaca gcataagtag
     3241 tatctataaa tgcagcagtc acatctggaa gagaagatgg aatttgagca atatctaact
     3301 ctttgaattg tattcccttt ggattttcta ttatgtcctt aggactaact aatccatccc
     3361 ctttaggtaa cgttaaaagt ccaatacttt caaggtatct aaggttctta gaacaagaag
     3421 atgggttatt gcttattgca attgtatctc cttttttaaa ttcatcaaca gattttatct
     3481 tataagagta aagtattgct gggcatacat atagtttttt accagccatt attttgtaac
     3541 ctttggcctt aacagcttct tttagataag gttcatgttg aaatagattt gcatcaattt
     3601 caccattatt taaagcttcg tttggaagta tgtaatcatc aaagttttta acttctaaag
     3661 tatatccttt ttctttaata agtggtttta attcttctaa taactctcct cctggaacta
     3721 aagtagcacc aacaacaatt tttttatcat ctttagcttt agaacatcca accacagcta
     3781 aagttagtac tagacataat aacaaacata ataatttttt gaatttcata taaattccct
     3841 cctgaatata attgattagt taaaaataaa ataccattct ttatcacact gcaaaataaa
     3901 aataagcctg agtttaaact caggctttaa aagctaattt agactcatct ttcagtataa
     3961 tactgcagga tttagcacca atgtatataa aaatatactg gttgctgggt ttcatagggc
     4021 cagtcccttc accactctgg ataagaagta tcaattttta attatgttat aaatattatc
     4081 atgatttttt acgtctgtaa acataatttt ataaatattt taaaatcttt aatgtatttt
     4141 atttaacacc taaaattcca gaaactagaa tatcagtatc agcacctaag aaatagtcat
     4201 ttatttcaat agaatccaga gtatttctca aagcattttc ttcatgcctt ataccaataa
     4261 gtttattttc tacaaatgca acatcacatt ttccaaaaaa atctccaaaa aattttatac
     4321 tagttattat gcctttatca acatttaaat taaactcaac atttccacca gaatatttta
     4381 attcatttga aagagtatat tttggtgaat taccaaaatt ccaatcccaa gtttcgtatt
     4441 ttgatttagt aagcttttct atttgtttta tatcatgctc actcaaacaa taattagtat
     4501 tatcagtatt tgttttagaa agataatcca ttatagcatc tttaaattct aatatgtcca
     4561 tttttgaatg taaatgttca cttatattgg ttacacgaga ttttacagat tttattcctt
     4621 ttccttcaaa ttttataggt ttaaccttta atgcttttga gaggtcattt atttcagatg
     4681 aaaataaaag agtcccatga tgcattactt tgtttttgta gttgtattga gcatttccag
     4741 aaaatttctt gccatctata agaaggtcat ttcttccaga aaattcggca ttgacatcta
     4801 gtgttttaag taaatctatt atgggctgag tgaatcttct gaaatcacta aaattattat
     4861 tgttacaagc tataaatgta aaatttatat ttcccaaatc atgaaataca gctccaccac
     4921 cagactgtct cctaacgaca ggtatagaat attcttttat atattctaaa tttatttcag
     4981 aaagtgtgtt ttggtttttt ccaacaatta tagaaggttc attcctccat aaaataaata
     5041 agtcttcatc aaaatttttt agaagatatt cttccattgc taaattaaaa taagggtttg
     5101 ttttttcatt ataaattaat aacattaaag tagttcctcc atagaattga taaaattttt
     5161 attatgaata caattaattt tataattcga caataatata aaaactcctt taaattagaa
     5221 attttaaaaa ctaccttgca aagtttaaat aaataatata tactcaaaat atgtttaata
     5281 aaagtgaata gaagggtaga ggcgcaataa tctatcagta ctaataagga gttaagcaca
     5341 gagagttatt aggaaaggag atattgccga agttttataa tcgatgcttt aagattatat
     5401 tgctgggggt acatagaaaa tatgtacaac tgtcacaggt ttatttgtgg tgagctatca
     5461 tttaaggaat tatattatat ttatttttac tattgttgat atattgtaat gtttaagcct
     5521 tgagtgttta ccactcaagg cttttttagt tttattgtaa cataataccc tgtacagttc
     5581 acaaaattaa aaggagaggg agatttatat gaggaacaaa aaaattaata ttatttttct
     5641 gacgacaatc atgttcataa tgtcaacagt aatggttttt gcagaagaag atatagacac
     5701 tattgcacta gccaatgcag aaaaatttgg gattttaact ttaataccac cattagttgc
     5761 tataatactc gcatttataa ctaaaaacgt aataatttct ttacttatag gtattctttc
     5821 tggtagtttt ataattaaag ctagtggtat taatgttttt gcgacattta tacaggcatt
     5881 tttagattta gtagatagag ctttagtatc tttagcagac ccttggaatg cggggataat
     5941 actacaggtt ttgtcaattg gaggagtaat aaatcttgtt gctaaaatgg gaggagctaa
     6001 agctatagct gaagcacttg ctaaaagagc aaagtcagca aaagggacac agcttataac
     6061 atggtttttg gggcttttag tattctttga tgattatgcc aattcactaa tagtaggtcc
     6121 tatgatgaga cctgttgcag ataaaatgaa aatatcgaga gaaaagctag catttataat
     6181 agatgccaca gcagcaccag ttgctggttt agctataata tctacatgga taggtcttga
     6241 ggttggttta atacatgatg cttttgaaag tataagtata gatgtagatg catttggtat
     6301 atttttaaat acaataccat ttagatttta caatatatta atattagctt tcattgtaat
     6361 atcagcatta ttattaaaag aatttggtcc aatgagaaaa gctgagataa aatctagaag
     6421 tagaaaaatt agtatagacc ttgatgaagg cgtagaagag ttggatgatt tagctccaaa
     6481 aaatggagta aaattgagtg tatggaatgc tataatccca ataggaacat tgataatagt
     6541 tgcattagca tcattttatt atagtggata tacatctata atgggaggag acgataaggc
     6601 tctgatacaa ctatttacaa attctccata ttcttttgaa gcaataaaag aagcatttag
     6661 tgcatctgat gcatctagag cattattcca gtctgcactt gttgcaagtt tagttgctat
     6721 tataatggct gtagtaaaaa agatttttac aatatcagaa gcaattgatg tttggataga
     6781 tggaatgaaa agtttagtaa tcactggtgt aatattaata cttgcatggt cattaagttc
     6841 agtaataaaa gagttaggta cagctaaatt tttaatacac ctactatcag gttcactacc
     6901 accattctta ttaccaagtt taatatttgg tttaggagca ataatatctt ttgctactgg
     6961 aacagcttat ggaacaatgg gtatacttat gccacttgca attccattag catactcatt
     7021 aaatcctgat atgtcttatg taatagtaag tactagtgca gttttaacag gtgcaatatt
     7081 tggagaccat tgttctccaa tttcagatac aacaatactt tcttcaatgg gagctggctg
     7141 taatcatata gaccatgtta acactcaaat gccatatgct atatttacag cagtaatcac
     7201 tattgtattt gggtatatac cagctggact tggattgcca atatatatag tattaccagt
     7261 ggcaatagct gctatatttg taggtattca aataattggt aagaaagttg atgaagcgga
     7321 aatagaactt gtagaataaa gatataaata agtttcacct tttattatca ctattttgtt
     7381 agtttttaga taattttaaa aagttattga aaataatggc gaaatatagt aaaataaaag
     7441 agtgataagc ggatgtggcg gaattggcag acgcactaga cttaggatct agcgccattg
     7501 gcgtgggggt tcgactccct tcatccgcac tttacagtac ttaattgtac tgaaaaaaac
     7561 tggggttgct ggctttcgga gtgggctatc ccgtttttat ttttttgtat atataataga
     7621 ttggcacttt agtataaggt gcttttttta taggcaaaaa cagtacattt atttctgttt
     7681 tattatcatt catctattag aatatacttt agttaagaat gtgttaataa gcagtgttca
     7741 cattcttgat aaaatgtgga ataatatgta tttttactaa gacggaaatg aaataattaa
     7801 accattaaat aatattataa gatgatgaat aagtagataa atcaaatttt gtaggaggat
     7861 attatggaaa aatatatatg gttgtttcca ttacttttta tttttcatga tatggaagag
     7921 ataataggtt ttggaatatg gcttaaaaaa aataaatcaa tgcttgataa gaagtatcca
     7981 tttatcagta aagtttatga aaattatagt actgaaggca tggcatttgc tgtgtttgaa
     8041 gaatttattt tatgcattat tttctgtata cttacagtta taacagagaa tcagtatgta
     8101 tatctactat ggttaggtag ttttatagca tataccttac atttggtaat tcatataggg
     8161 cagtcaatca taataagaaa atatatacct tcacttatta ccagtatcat atgcttacca
     8221 ataagcattt ggtgtataag taagagtatt tatatagttg attgtgaaat gagtacaaca
     8281 attctgtaca gtataattgg cattataatt gtagcattga atttaaagtt tgcacagtcg
     8341 ctgattggaa aatttacaaa gtggatgtca aacatataaa ttttagttta ttggacagtt
     8401 caaggaatca gaaattggaa ttttatagta ttaatatttt atggtattaa tataatgtat
     8461 aaaattttat agcaatgtat ttcataagta taaagtgact agaattttaa gaaatataca
     8521 aattctataa aaactaaata atttcaagta tattttaaaa atcttatttt tcacaagttt
     8581 aaaattgtaa acgatttcat caagtctatg aatatacttt taacatgtta tagtaaatct
     8641 caatataaat ttacatttgg ttctttgtgt gttattttaa taataaatta agtgaaaatg
     8701 ttattttaca ttaatatata atatctttgc ataagagtga ataatataaa agagaaataa
     8761 ttgataataa aaacttaaca aaaaaattaa tacataaaaa aaaataaata taatgtgttg
     8821 acatttaatt gaaatattca tacaataaaa ttgaaaataa atatttccaa atgagattag
     8881 cgagagagtc ctttatacga aaaggcgccg aagaagaaat ttaagaaatg ccaattttta
     8941 gagaagcttt caggcaaaaa tatcgctaat tgatgggact ctggagagac ttaagttaag
     9001 atactataag tcaccgaaga agcaaaataa tattaaatta ttaaatcttt caggtataca
     9061 aacagggata tataggctag gattattttc catgctgata tatctttttt ttatgaaaaa
     9121 gtacaggaaa taatatcaaa gttacttaaa tacagtaggt ctaattttct agggagggtt
     9181 atatatgaat gaattgaaaa gagtatcttt gtacaatata cacaaagagc ttggagcaaa
     9241 acttgttgaa tttgctggtt gggaaatgcc tttagaatat gagggtataa ataaagagca
     9301 tgagaaggtc agaaaaagtg caggtatatt tgatgtatct catatgggag aggtacagat
     9361 aaagggagca gagtcagaaa aatttataca aaacctagtt acaaatgata taagcgcatt
     9421 aaagataaat gatattatat atacacctat gtgttatgaa aatggtggag tagttgatga
     9481 tttattaata tataaatttg gtgaagaaga ttatttatta gtaataaatg caggtaatat
     9541 agataaagat gtagcatgga taataaaaca aagtgaagga tacaatgtag atataaaaaa
     9601 tatatctagt gaagtgagtc aattagcaat tcaaggacct aaagcagaag aaattcttca
     9661 aaaaatcaca gatatagact taaactcaat aaaattttat aagtcaattc catcaacaaa
     9721 agtatgtggt tgtccttgtt tagtttcaag aacaggatat actggggaag atgggtttga
     9781 gatatactgt aaaaataaat atgtagaaat aatttggaat gaagttctaa aggttggagg
     9841 agaagatata tgtccagctg gtctaggttg tagggatact ttgagatttg aagcagctct
     9901 acctctatat ggacatgaaa taaatgaaca catatctcca attgaaggtg gtttatctat
     9961 ctttgtgaaa acaaataaag aatcatttat tggcaaatca attttatcaa aagaaaaaga
    10021 aagtggagct aaaagaaagt tagtaggatt tgaaatgcaa ggtaaaggaa tgcctagaaa
    10081 tggatatgat ataaggattg gtgataaaac agttggattt gtaacaacag gatgtgcatc
    10141 tccaacgaca ggaaaaattt taggtatggg aattatagat tctgaatatg caaaagtagg
    10201 aaatgaaata ggtatagcaa tcagaaaaaa agttgttcct gctgtgatag ttaagaaacc
    10261 tttttataaa aaacaatata aaaaagataa tattatatta aacaaagaaa ataaattttc
    10321 atatatacct gctacaagtg aagataaatc aaaaatgtta aaagtagttg gattaaattc
    10381 agttgatgaa ttattttcag atataccaga agaagtaaaa ttaaaaagag atttaaattt
    10441 ggaaattgga aaatctgaat tagaggtaag caaaatagta aaaagattgt cagaagaaaa
    10501 tttaagttta gaagacttga cttgttttct tggtgctggt gcatatgacc attatatacc
    10561 atcaattata aaacatataa cttcaagatc tgaattttat acagcatata ccccatatca
    10621 agctgaaata agtcagggaa ctttacaagt agtttttgaa tttcaatcta tgattgcaga
    10681 aattacaggt atggaaattg caaatgcatc aatgtatgat ggagcaactg ctgcaataga
    10741 agcatgtata atggcaatga atcagactag aaaaagtaaa atagtagtgt caaaaactat
    10801 tcatcctgaa actctgtcag tattaagaac ttacttacaa tataaagatt gtgagatagt
    10861 agaaattgac ttttgtaatg aatatgggac tacagatata gaaaaattaa aagcttctgt
    10921 agacaaagat acagcatgtg ttcttataca aactcctaat ttctttggaa ttattgaaga
    10981 aatggaagaa atagaaaaga taactcatga aaataaggct atgttaatta tgagtgtaga
    11041 cccaatatca ttaggagttt taaaaacacc aggtgagata ggtgcagata ttgttgttgg
    11101 agaagctcaa tcacttggaa accctcttaa ttttggaggt ccttatgtag gatttttagc
    11161 tagcaaatct aaatatacta gaaaaatgcc aggaagaata gttgggcaaa gtttagatgt
    11221 agaaggtaaa attgcatatg tattaacttt acaaactaga gaacagcatg taagaagaga
    11281 aaaagcaact tctaatatat gttcaaatca agctttaaat gctcttgtag cttcaatata
    11341 tatggctact atgggtaaag aaggatttaa agaggttggt atgcagtcta tgaaaaaagc
    11401 acattatacc tacaataaac ttgtacaaac tggaaaatat aagccaatat ttaaaggtaa
    11461 gtttttcaaa gaatttgctg tacaaggaaa tcttaatata gaaactataa atgataaact
    11521 cttagaagaa aatatattag gaggttataa tttagaatac aattatccag aattaaaaaa
    11581 ttcaactttg ctttgtgtga ctgagaaaag aagcaaagag gaaatagata agttagttgg
    11641 aataatggag ggattataat gaaagaatac aatagtttgt tgatagatat atctaaaaaa
    11701 ggtaggaaag catactcact tccaaagtta gatatagatg atataaaaat agaaaatatg
    11761 attgaccaag atatggcaag acaaagtgag ttgaatttgc cagaagttgg tgaattagaa
    11821 ttagttagac attatacatt attatcaaat aaaaactttg gagttgatac aggtttttat
    11881 cctttgggtt cttgtactat gaaatataat cctaaaataa atgaagatat ggcagcactt
    11941 ccaaacttta ctggaatgca tccataccaa tcttcggata cagctcaagg ttctctttct
    12001 ttgatgtatg atttatcaag aagacttgca gaaattacag gtatggatga agttacacta
    12061 caaccttcag caggttctca tggtgagttt acagggctta tgattataaa agcatatcat
    12121 gaaaatagag gtgaccataa gagaaccaaa gtgataattc cagattctgc acatggaaca
    12181 aatccagcaa gtgcagctat ggcaaacttt gatgtgattc aaatagcatc tgataaaaat
    12241 ggagctgtag acataaatgc attaaaagag gttttgaatg atgaagtagc agctcttatg
    12301 cttactaatc caagtactct tggtttattt gaaaaaaata taaaagaaat agctactctt
    12361 gtacatgaag caggaggact tttgtactat gatggtgcaa acatgaacgc tattatgggg
    12421 ataacaagac ctggagatat ggggtttgat gtggttcatc ttaatcttca taagactttt
    12481 tcaactcctc atggaggtgg aggtccagga gctggtcctg ttggagttaa aaaagaatta
    12541 gtaccttttt taccaatacc agtaattgaa agaaaagaag ataagtatgt actaaattat
    12601 gatagagaaa aatctattgg gaaaattaaa aatttctatg gtaattttgg agttcttgta
    12661 agagcttaca catatatact aactatggga agggatggac ttaaagaggc tagtgaaatg
    12721 gcagttttaa atgctaatta tataaaggaa agtataaaag atgattatat tttgccaata
    12781 gataccttgt gtaagcatga gtttgtatta ggtggattgc caagaggaga ggccaatata
    12841 aaaactatcg atattgcaaa aagactacta gattatggat accatcctcc aacaatgtac
    12901 tttcctctta ttataaatga ggcacttatg atagaaccta cagaaagtga gagtatagag
    12961 acattagata gttttataga agctatgaaa agtgttgcta aagaggcaaa ggaagaacca
    13021 gaactattga aaactgctcc acataatact ttggtcaaaa gagtagatga tgcaagggca
    13081 gtaaagaaac ctatactaac atggtcacaa agataaatag ttaaaaagta gagatttaaa
    13141 gtataaagag aattaaattt gtaagaaaaa gctatctaac ttaatctagt ttagatagct
    13201 tttttgtata ttagtatgta tggtattttc tatataaagt tttaaattat attagtgctt
    13261 atatagaata ttgatatgat gattatgtat agatattaat aagaattgtt gaatttatct
    13321 tgccttaact ttatttactc ttttatatta agaaaaactt aactaaaata tagcgaaatt
    13381 cataaaaatt taggaaatct t
//
LOCUS       DAMJVK010000102        13117 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.106, whole genome shotgun sequence.
ACCESSION   DAMJVK010000102 DAMJVK010000000
VERSION     DAMJVK010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13117
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.106"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            42..506
                     /gene="bcp"
                     /locus_tag="PC414_003092"
     CDS             42..506
                     /gene="bcp"
                     /locus_tag="PC414_003092"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HDF2465471.1"
                     /translation="MLSIGTKAPEFTLEDKDGNKVSMSDFKGKKVVVYFYPKDNTPGC
                     TRQACAFRNAYDGFKKEDIQVIGISKDSIKSHQKFAEKHELPFILLSDPDLVAIKAFD
                     VWKEKKMYGKTALGVVRATYIIDENGIIEKVFEKAKPDTNAQEILEYLEKQE"
     gene            516..1259
                     /gene="yaaA"
                     /locus_tag="PC414_003093"
     CDS             516..1259
                     /gene="yaaA"
                     /locus_tag="PC414_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HDF2465472.1"
                     /translation="MIIMLSPAKNMKNIEVFDRDLSLPCFIDNTKEIVENIKTFGIED
                     FKNKMKINEKLAVLNKNRFESIKFDRLGNPAILTYDGIQYKNIEAENFTRKDEEFANS
                     CIRIISGLYGVVKPYDSIYEYRLEMQTKLQVGEFKNLYEYWGNRIYKELIKEKTAIVN
                     LSSNEYSKSIEKFIKDSDTYITCTFKVNKNGILKVESTQAKKARGMMTKYIVKNRIRD
                     IEELKKFDLEGYKYKENLSNNSEYIFVKE"
     gene            1387..4044
                     /locus_tag="PC414_003094"
     CDS             1387..4044
                     /locus_tag="PC414_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDF2465473.1"
                     /translation="MENHKQMPWHSRATHEILESLDSSEEGLTDTEAEKRLKENGLNE
                     LRKKPPRTTLQMLWSQITDAMVMILIGAAILSLIFGEFTEAFVILIIVTVNAVIGIVQ
                     EKKAESSLEALKNMNSPTARVMRDGEENVIPASNLVVGDIVFLEDGAMVPADLRLIET
                     SSLKIQEASLTGESVPSEKDADTILPKECVLGDRSNMAYTSSIVTYGRGVGVVVATGM
                     DTEVGNIAHLLDSQDDFDTPIKRKLNTVGKTLSVIGIIVCVVIFAIGAFYQRPLLPMF
                     MIAISLAISIIPEGLPATATIVMALGVQRMAKRNALIRKLPAVETLGGATVICCDKTG
                     TLTLNKMTVTHIAVNGDFESGKVTSIDTASEKHPLVYKEIVYAGALCNDACINPDKTD
                     EILGDPTEGALIFMAKKFGIDQEALEEEYPRVFEQPFDSDRKCMTTVHNIDEKIIAYT
                     KGAVDEILDLCTKILTSKGERNITEVDKKNIHELCLNMSKDALRVLGFAKHEISSIPK
                     EDSENIEYDLTFIGMVGMIDPPRTEVIDAVSTCKEAGIRTIMITGDHKVTATTIAHEL
                     GIWSEENTVISGDELDNLSDDELDEAVKNTTVFARVSPFDKLRIIQSLKRIGEVPAMT
                     GDGVNDSPALKSADIGIAMGISGTDVAKDSSDMILMDDSFTTIAYAIKEGRRVYRNIQ
                     KVIQFLLVGNIAEILTLFVAILLNWDTPLLAVHILWVNLATATLPALALGVDPPSRNI
                     MKHQPVKSTTLFEKNLIQRVVTQGIFVAIMTILAYKIGLNIENHSVGQTMAFCVLAFS
                     QMLRAFSQRSNTDSMFNRNNGKNPFLLISFLTSAFLIAVILFVPVFRNAFNLTVLNNL
                     EWLVTIVLAIMSVIQVEVGKIIKRKKLKK"
     regulatory      4098..4200
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            4310..5575
                     /locus_tag="PC414_003095"
     CDS             4310..5575
                     /locus_tag="PC414_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HDF2465474.1"
                     /translation="MYNKETICVQGNYKPGNGEPRVLPLYQSTTFKYSSIDQLAELFD
                     LKVDGHIYSRISNPTIQAFEEKISLLEGGVSSVAVSSGQSANMLAVLNICKSGDSILC
                     SSKVYGGTFNLLGPSLKKFGIDLISFDLDSSEDEIVELAKENTKVVFAETLANPTLEV
                     IDFEKIANVAKRINVPFIVDNSLASPVLCNPLKYGANIVTHSTTKYLDGHASSVGGII
                     VDGGNFNWDNGKFPELVEPDPTYHGISYTQKFGNAAYATKARVQLLRDYGNCLSPFNA
                     YLTNLNVETLHLRMERHSENALKIARFLEKHENVDWINYPGLEDNKYYENAKKYLSRG
                     CSGVLSFGVRGGLENAKKFVEKLQIASLVTHVSDVRTCVIHPASTTHRQLTEEQLIAS
                     GVLPSLIRLSVGIENVEDLIADLNQALNF"
     gene            5605..6495
                     /gene="metA"
                     /locus_tag="PC414_003096"
     CDS             5605..6495
                     /gene="metA"
                     /locus_tag="PC414_003096"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HDF2465475.1"
                     /translation="MALILPKGLPVINKLLDEGIDVIYKEDFKKELKYEENIDTKIAI
                     LNLMPIKIDTELDLLRRIDKTGFNVSVEFIKISTRESKRSCNEYVKEFYKTFDEAKGE
                     YFDGFIITGAPVEQMEFEEVDYWNELEEIMDYSKRKTKSTLYICWAAQASLYKYYNVK
                     KLPLSQKCFGVFKHKVDKDSKIVDGFENEFFAPHSRHTTVNIEALKENKELSIVSHSK
                     EAGPYIITNSRDVFVMGHSEYDKYTLDKEYKRDINKGDKISIPQNYYINDDPSEEPTV
                     KWKKHSELLFRNWIKNYLIQ"
     gene            6986..7429
                     /locus_tag="PC414_003097"
     CDS             6986..7429
                     /locus_tag="PC414_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2465476.1"
                     /translation="MDEMSFKKELLELTRDINMKFTTLLSDFYQPLGITAVQALILSE
                     LCEHGEKKISDLGKNLNMTNSNVSVICQRLEKNGFLNRIRDIEDQRIVKVKVTNKSLD
                     IQEHISSSIFDSYFENMTSEKLQDMEDIIEGLEKLNKLLTYIDCK"
     gene            7460..9277
                     /gene="ftsH"
                     /locus_tag="PC414_003098"
     CDS             7460..9277
                     /gene="ftsH"
                     /locus_tag="PC414_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDF2465477.1"
                     /translation="MDEHKPQKRSALYYYTVIIIVVMFLNALIFPSLMDKKMVEIDYG
                     KFLTMLDTGKVKSVEIENNRIAINPSSSKDKNIYITGRMDDPELVDRLKNAKVEFTKV
                     IPKENSPLLSILLTWIIPIGILMLFGNLMMKSMQKRMGGNAMQFGKSNAKMYVSAQSG
                     KRFNDVAGQDEAKEALTEIVDFLHNPEKYKKIGAQMPKGALLVGPPGTGKTLLAKAVA
                     GEANVPFFSISGSEFVEMFVGMGASRIRDLFKQAKEKAPCIVFIDEIDTIGKKRDNGS
                     GLGGNDEREQTLNQLLSEMDGFDGGIGVVILAATNRPDSLDKALLRPGRFDRRIPVEL
                     PDLGGREAILKVHAQNVNVSSDIDYNAIARSTSGASGAELANIVNEAALLAVKSGRDI
                     VEQQDFDESVETVIAGYQRKGAVISDKEKKIIAYHEIGHALVAAMQKHSAPVHKITII
                     PRTSGALGYTMQVAEDESVLMSKEEAIDKITTFTGGRAAEEVIFNTCTSGASNDIEQA
                     TKIARAMVTRLGMSEEFDMVALETVSNQYLGGDTSLACSSDTAAKIDEEVFKIVKACH
                     KNAIEILENNIDKLHELANYLLEKETITGDEFMEILNLN"
     gene            9455..12157
                     /locus_tag="PC414_003099"
     CDS             9455..12157
                     /locus_tag="PC414_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HDF2465478.1"
                     /translation="MDRPNPDILLEKIKNEEEKLSRGQLKIFFGYAAGVGKTYSMLES
                     AQNLKKVGVDVVVGYIEPHTRPETLALLDGLEILPVKEIEYKSIKLKEFDLDAALARK
                     PEVILVDEFAHSNVAGLRHTKRWQDIEELLLAGINVYTTVNVQHLESLNDIVEIITNV
                     SVKETIPDKFLDTNTQLELIDVEPDVLLERFNEGKIYKKEQATRAKNNFFIKDNLVAL
                     REIALRKTAERVNKEVQMTRLSKGDVTVIPTSDTLLACISPSPSSAKVIRTASRISDS
                     TFAKWIALYVETPNTARLPFDEQKQLQNNLKLAKKLGGEIIVLHGENIIEQILRIAKL
                     RNVTKIVIGRNHSSNKRFSKRFKKDIVDKLIDEVDYIDIHIIPYKFASDVKYKPKKDK
                     SSIKSKFKISKVDFIKLIFITLMISILAYAVQSMGFIRENILLIYMLGVVLVSMWTKG
                     YSTGIISSVFNIVLLNYFFTAPLYTLSIADSNYIVTLVVFSIVGIITSTLTSKIQHEA
                     ETAAKREENTKMIYQIIKGFLRLSNKDDIVNKGIELLSLSLSRDVICYLNVDKHNKSK
                     LYKKNTNNKDKNDLDSEDEKAVASWVLSNSTVAGNDTDTLPGSKGYYIPIVGMNLTLG
                     VVGISCIDSKLDTEDISLIETIIAQMAIALDREILSEAKENTNLEIERERLRSNLLRA
                     VSHDLRTPLAGISGAVSTIIKNKGTIGQDIIDELLSGVYEDTQWLIRLVENLLSMTKI
                     DEGKLEVKKHSELVEEIISEALQKIKKRIENALIDINIPEQILFVPMDAKLIEQVLIN
                     LIDNSLKYSKEDCKIEINVYEKDDYVWFEVSDNGPGISKELKKHIFDRFFTGEEGAKD
                     SRKGVGLGLSICKSIIQAHKGEIMVENNKDKGSTFKFSLPKENE"
     gene            12191..12889
                     /locus_tag="PC414_003100"
     CDS             12191..12889
                     /locus_tag="PC414_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2465479.1"
                     /translation="MSKKLILLVEDDKTIRKFISTALLTQDYDVKEAITGKEGISIAV
                     SYSPDVVLLDLGLEDMDGIEVIKAIRQFSNIPIIVVSAREQDRDKVEVFDAGADDYLT
                     KPFSIVELLARVRVAFRHSQVEAQQKDDVKSTFEVDKLLIDFDKRKVIVDDVEVHLTP
                     IEYNILSLLAKYHGKVLTHNFIIKEIWGSVIGNETKSLRVFMATLRRKIEKQPANPRY
                     IITEVGVGYRLNDE"
BASE COUNT         5150 a         1504 c         2398 g         4065 t
ORIGIN      
        1 atagtataaa attattaata aaaacaaaag gagagatatt tatgttgagt ataggaacaa
       61 aagcaccaga atttacacta gaagataaag atggaaataa ggtaagtatg tcagatttta
      121 aaggcaaaaa agtagtagta tatttttatc ctaaagataa tacaccagga tgtacacgtc
      181 aagcttgtgc ttttagaaat gcatatgatg gatttaaaaa ggaagatata caagttattg
      241 ggataagtaa agatagtata aaatcacatc aaaaatttgc agaaaagcat gaacttcctt
      301 ttattttact ttctgaccct gacttagttg ctataaaagc ttttgatgta tggaaagaga
      361 aaaaaatgta tggaaaaact gcattgggag ttgtacgtgc gacatatatt atagatgaaa
      421 atggaattat tgaaaaagtc tttgaaaaag caaaaccaga cacaaatgca caagagattc
      481 ttgaatattt ggaaaaacag gagtaataag aatttatgat tataatgtta tcaccagcta
      541 aaaatatgaa gaatatagaa gtatttgata gagatttgag tttgccttgc tttatagata
      601 acacaaaaga aatagtggaa aatataaaga catttggtat agaagatttt aaaaataaaa
      661 tgaaaattaa tgaaaaatta gctgtattaa ataaaaatag atttgaaagc attaaatttg
      721 atagattagg aaaccctgca atcttaactt atgatggaat acaatataaa aatatagaag
      781 cagaaaattt tacaaggaaa gatgaagagt ttgcaaatag ctgtattcga atcataagtg
      841 gattgtatgg tgttgtaaaa ccatatgata gtatatatga atatagatta gaaatgcaga
      901 caaaattaca agtaggtgag tttaagaatt tatacgaata ttggggaaat cgtatatata
      961 aagaactaat caaagaaaaa acagctatcg ttaatctttc atcaaatgaa tacagcaaat
     1021 ccatagaaaa atttattaaa gatagtgata catatattac atgtactttt aaagtaaata
     1081 aaaatggcat attgaaagtg gaatctacac aagcaaagaa agctagaggt atgatgacaa
     1141 aatacattgt aaaaaatagg ataagagata ttgaagaact gaaaaagttt gatttagaag
     1201 gttataagta taaagaaaac ttatctaaca atagtgaata catatttgta aaagagtaga
     1261 agttttgtta aatattaaat ggcattgtta aatttaggta aaatatactt agtgtgtatt
     1321 gaattgaact tgattttaaa atataattat tcttaaatta aaaaaagaaa ggagtggtct
     1381 tccaaaatgg aaaatcacaa acagatgcct tggcattcaa gagcaacgca tgaaatttta
     1441 gaaagtctag attcatcaga ggaggggctc accgataccg aggcggaaaa acggcttaaa
     1501 gagaatggct taaatgaact tagaaaaaaa ccacctagaa caactttaca aatgctttgg
     1561 agtcaaatta cagatgctat ggttatgata ctaataggag cagcaatatt atctttgata
     1621 tttggcgaat ttacagaagc ttttgtaata ctaataattg taacagtaaa tgcagttatt
     1681 ggaattgttc aagagaaaaa agcagaatca tcattagaag cattaaaaaa tatgaattca
     1741 cctacagctc gtgttatgcg tgatggtgaa gaaaatgtta ttccagcaag taatcttgta
     1801 gtaggagata ttgttttttt agaagatgga gctatggttc cagcagacct tcgtttaata
     1861 gaaacatcaa gtttaaaaat tcaagaggct tctttaacag gagaatctgt tccatcagaa
     1921 aaagatgcag atacaatctt acctaaagaa tgtgtattag gagaccgtag caatatggcg
     1981 tatacatctt caatagtcac atatggtaga ggtgtaggtg ttgtagtagc aactggtatg
     2041 gatacagaag tgggtaacat agcacatctt ttagattcac aagatgattt tgatactcct
     2101 ataaaacgta aacttaatac tgttggaaag acattaagtg tcataggtat tattgtatgc
     2161 gttgttatat ttgcaatagg tgcattttat cagagacctc tcttgccaat gtttatgatt
     2221 gcaatttccc ttgccatatc aattattcca gagggattac ctgcaacagc tactattgta
     2281 atggctttag gcgtacagcg tatggctaaa agaaatgctt taattagaaa attacctgct
     2341 gttgaaacat taggaggagc tacagtaata tgttgtgata aaactggtac attaacctta
     2401 aataaaatga ctgttacaca tattgctgtc aatggagatt ttgaatctgg aaaagtgaca
     2461 tcaatagata ctgcatctga aaaacatcca ttagtgtata aagagattgt ttatgctggt
     2521 gcactttgta atgatgcatg tattaatcca gataaaacag atgaaatatt aggtgaccca
     2581 acagagggcg cattaatttt tatggccaaa aaatttggaa tagatcaaga agctcttgaa
     2641 gaagaatatc cacgtgtatt tgaacagcct tttgattcag atagaaaatg tatgacaact
     2701 gttcataata tagatgaaaa gattattgct tatactaaag gagcagtaga cgaaattctt
     2761 gacctatgta ctaaaatatt aacttcaaaa ggtgaaagaa acattactga agtggataaa
     2821 aagaatatac acgaattatg tcttaacatg tctaaagatg cattgcgtgt acttggtttt
     2881 gctaagcatg agatttcatc tataccaaaa gaagatagtg aaaatataga atatgattta
     2941 acatttatag gtatggttgg tatgattgac ccaccaagaa ctgaggtaat tgatgcagta
     3001 agtacttgta aagaagcagg tataagaacc ataatgataa caggtgacca taaagttact
     3061 gccacaacaa tcgctcatga gttaggaata tggagtgaag aaaatactgt gatatctggt
     3121 gatgaacttg ataatttgag tgatgatgaa ctagatgaag ctgttaaaaa tactacagta
     3181 tttgctcgtg tatccccatt tgataaactt agaattatac aatctctaaa aagaattgga
     3241 gaagtaccag caatgactgg ggatggtgta aatgactctc ctgccttaaa gtctgctgat
     3301 attggtatag ccatgggtat aagtggtaca gatgttgcaa aagattcttc tgacatgatt
     3361 ttaatggacg atagttttac aacaattgct tatgcaataa aagaaggtag acgtgtatat
     3421 agaaacatac aaaaagttat acaatttttg cttgttggta atatagcaga gatacttacg
     3481 ttgtttgtag caatattatt aaattgggat actccgcttc ttgctgttca tattctatgg
     3541 gtaaaccttg caactgctac gcttccagcc cttgctcttg gagttgaccc accaagcaga
     3601 aatataatga agcaccagcc agtgaaatca accactttgt ttgaaaaaaa tcttatacaa
     3661 agagttgtta cccaaggtat atttgtggca ataatgacta ttttagcata taaaattggt
     3721 cttaatatag aaaatcattc tgtaggtcaa acaatggcat tttgtgtatt agcattttca
     3781 cagatgttac gagcatttag tcagcgttca aatacagatt caatgtttaa tagaaataat
     3841 ggtaaaaatc catttctttt aatctcattt ttaacttctg catttttaat agcagtaata
     3901 ctatttgtac ctgtttttag aaatgctttt aacttaacag ttttaaataa cttagagtgg
     3961 cttgtaacga ttgttttagc cataatgtct gtaattcaag tagaggttgg gaaaattatt
     4021 aaaagaaaaa aattaaaaaa atagttgaca aaattaggaa aaatatataa gatataaaac
     4081 atagaaaaat taaataaatc ttatccagag aggtggaggg actggcccta tgaaacccgg
     4141 caaccagtat atttatatac attggtgcta aatcctgcgg cgaaagctga gagatgagta
     4201 taattaagct tatacagagc ctgagttata tactcaggct ttatttattt taactctcat
     4261 ctggtaaata gtttaatttt ttttactaaa atctaagggg gatacaagaa tgtataataa
     4321 agaaacaata tgtgtgcaag gaaattataa accaggtaat ggagaaccaa gagtactacc
     4381 tttatatcaa agtacaactt ttaaatatag cagtatagac caacttgctg aattatttga
     4441 tttaaaagtt gatggacata tatattcaag aataagcaat cctactattc aagcttttga
     4501 agaaaaaata agtttactag agggtggagt atcttctgta gctgtatcat cagggcaatc
     4561 tgcaaatatg ttggcagttt taaatatatg taaatcagga gatagtatac tttgttcttc
     4621 aaaagtatat ggaggaacat tcaatttact aggacctagt cttaaaaaat ttggtataga
     4681 tttaatatcg tttgacttag attcaagtga agatgagata gtagaacttg caaaggaaaa
     4741 tactaaggtt gtgtttgcag aaacacttgc aaatccaact cttgaagtca tagattttga
     4801 aaaaatagca aatgtagcta agagaattaa tgttccattt attgttgata attcattagc
     4861 atctccagtg ctttgtaacc ctttaaagta tggagcaaat atagttactc attctaccac
     4921 aaaatattta gatgggcatg cttcaagtgt tggaggaatt atagtggatg gtggaaactt
     4981 taactgggat aatggaaaat ttccagaatt agttgagcca gacccaacat atcatggtat
     5041 aagctatact caaaaatttg gaaatgccgc atatgcaact aaagcaagag ttcagttgct
     5101 tagagactat ggaaattgtt taagcccatt caatgcgtat cttactaatt taaatgttga
     5161 aacactacat cttagaatgg agagacatag tgaaaatgca cttaaaatag ctagattttt
     5221 agaaaaacat gaaaatgtag attggattaa ttacccagga cttgaagata acaagtatta
     5281 tgagaatgcc aaaaagtatt tatcaagagg atgtagtggt gttttatcat ttggagtaag
     5341 aggtgggtta gaaaatgcca aaaaatttgt ggaaaaatta cagatagcat ctttggttac
     5401 acatgtttca gatgtaagaa cttgtgttat acatccagct tcaactactc atagacaatt
     5461 aacagaagaa caattaattg catctggagt attgccttca ctaataagat tatctgttgg
     5521 aatagaaaat gtagaggatt taatagctga tttaaatcaa gctttaaatt tctaatattc
     5581 taagagaaag agaagtgaaa agatatggct ttaatactac caaagggatt acctgttatt
     5641 aataaattat tagatgaagg aatagatgta atttataaag aagattttaa aaaggaatta
     5701 aagtatgaag aaaacataga cactaagata gctatactaa atctaatgcc tataaagata
     5761 gatacagaat tagatttact aagaaggata gataaaacag gattcaacgt gtctgtggaa
     5821 tttattaaaa tttcaacaag agaaagtaaa agaagttgta atgaatatgt taaagaattt
     5881 tataaaacat ttgatgaagc caaaggagag tattttgatg gctttataat aacaggagct
     5941 ccagttgaac aaatggagtt tgaagaagtt gattattgga atgaactaga agaaattatg
     6001 gattattcta agagaaaaac taaatctaca ttatatatat gttgggcagc tcaagcaagt
     6061 ctttacaaat actacaatgt aaaaaaactt ccactaagtc aaaaatgttt tggagtattt
     6121 aagcataagg tagataaaga tagtaaaata gtagatggat ttgaaaatga attttttgct
     6181 ccacattcaa gacatactac tgtaaatatt gaagctttga aagagaataa agagttgagc
     6241 attgtgagtc attctaaaga agctggtccc tatataatta caaattcaag agatgtgttc
     6301 gtaatgggtc atagcgagta tgacaaatac actcttgata aagaatataa gagggatata
     6361 aataaaggag ataaaatatc tatacctcaa aattactata ttaatgatga cccttcagaa
     6421 gagccaactg taaaatggaa aaaacattca gaattgttgt ttagaaattg gataaaaaac
     6481 tatttgatac aataaatttt aagagtatat aaatgttaac taaaaaaata tagaaattta
     6541 taggtataaa gttaatctta atgatacttt ataccttatt tttttaaaat ttgaccttat
     6601 aatagtagtt ttatatttta agaaaatcac tttaggtgta tgtagataga ggtggcttta
     6661 attttatata tggtgcgata atgggagact aaacctttat agtatcaata aatttgtatt
     6721 ttaactttta aaagagatta aaaagggaga aatcagtgta aaatatatat atagattttt
     6781 tattaaattt tattaaaaat atcctgtata aaattaaata tcaataagat aacaaaattt
     6841 caattgaagt tacaatattg taattgacaa taaatactta atactataat atttaataca
     6901 ttaaacatta tagtgttaag tatttattta cagtatgcac ttcagcctaa atttgaaata
     6961 aagtattttc gtagggagcg tttgtatgga tgagatgagt tttaagaaag agctactaga
     7021 gttgactaga gatataaata tgaaatttac aactttatta agtgattttt atcagccact
     7081 tggtattaca gcagtgcaag ctttgatttt atctgagtta tgtgaacatg gagagaaaaa
     7141 aataagcgat ttagggaaga acttaaacat gacaaatagt aatgtatcag taatctgtca
     7201 aaggcttgaa aaaaatggct ttttaaatcg cataagagat atagaagacc aacgaattgt
     7261 aaaagtaaaa gttacaaata aatcattgga tatacaagaa catatttcaa gtagtatttt
     7321 tgatagttat tttgaaaata tgaccagtga aaaattgcaa gatatggaag atataataga
     7381 aggtttagag aaattaaata aacttttaac ttacattgat tgcaaataac ataaaaataa
     7441 tttaaaggag aataaaatta tggatgaaca taaaccccaa aaaaggtcag cactctatta
     7501 ttatacagtt ataataatag tagtaatgtt tttaaatgct ttaatatttc caagtttaat
     7561 ggataaaaaa atggttgaga ttgactatgg aaagtttctc acaatgttgg atacaggaaa
     7621 ggttaaatct gttgagatag aaaataatag aatagcaata aatccatcta gtagcaagga
     7681 taagaatata tacataactg gtagaatgga tgacccagaa ttggtagata gactaaaaaa
     7741 tgcaaaagta gaatttacta aggttattcc taaggaaaat tcacctcttt taagtatttt
     7801 acttacatgg ataataccta taggtattct tatgctattt ggtaacttaa tgatgaagtc
     7861 tatgcaaaag cgaatgggtg gtaatgctat gcaatttgga aaaagcaacg ccaaaatgta
     7921 tgtatctgct caaagtggaa aaagatttaa tgatgtagct ggtcaagatg aggcaaaaga
     7981 agctttaact gaaatagtag atttcttaca caatccagaa aagtataaaa agataggagc
     8041 acaaatgcct aaaggagcac ttttagtagg acctccaggt acaggaaaaa cactccttgc
     8101 aaaagctgta gcaggtgaag caaatgtacc atttttctca atatctggtt ctgagtttgt
     8161 tgagatgttt gtaggaatgg gagcttctag gattcgtgat ttatttaaac aagcaaaaga
     8221 aaaagctcca tgtatagtgt ttatagatga gattgatact attggtaaaa agcgtgataa
     8281 tggaagtgga ttaggtggaa atgatgaacg tgaacaaact ttaaatcagt tgttaagtga
     8341 gatggatggt tttgatggtg gaattggagt agtaatactt gctgctacaa atagacctga
     8401 cagtcttgac aaagcattac ttcgtccagg aagatttgat agacgtatac cagttgaact
     8461 tccagacctt ggaggacgtg aagctatatt aaaggtacat gctcaaaatg taaatgtaag
     8521 ctcagatata gattataatg caattgcaag gtctacatca ggagcatcag gtgcagaact
     8581 tgcaaatatt gtaaacgaag cagcactttt agcagttaag tctggcagag atatagtaga
     8641 acaacaggat tttgatgaat ctgttgaaac tgtaattgct gggtatcaac gtaaaggagc
     8701 tgtaatttca gataaggaaa agaaaattat agcatatcat gaaataggtc atgcacttgt
     8761 tgctgctatg caaaaacatt ctgcacctgt ccataagatt actataatac ctagaacttc
     8821 aggagcattg ggatatacta tgcaagttgc tgaagatgag agtgtactta tgtctaaaga
     8881 agaagcaatt gataaaataa ctacttttac aggtggtcgt gctgctgaag aggttatctt
     8941 taacacttgt acatcaggag catcaaatga tatagaacaa gctactaaaa ttgcaagagc
     9001 tatggttact cgccttggta tgagtgaaga gtttgatatg gtcgctcttg aaactgtaag
     9061 caatcaatat ttaggaggag atacttctct tgcttgttct tccgacactg ctgctaaaat
     9121 agatgaagag gtatttaaaa tagtaaaggc ttgtcacaaa aatgcaattg aaatcttaga
     9181 aaataatatt gataaattgc atgaacttgc taattattta ttagaaaaag aaactattac
     9241 aggtgatgag tttatggaaa tacttaactt aaactaaaat gattatacac aagataaaaa
     9301 tgcctattgt taggcatttt tattttacaa ctaaattttt taaatttata tttaaatctt
     9361 tacataatct taatcattaa aaatacatct taactacaat tttatatcgg aattgatatt
     9421 ataatagtaa tgaatggtgt aaggaggttt tttaatggat agacctaatc ctgatattct
     9481 attagaaaaa attaaaaatg aagaagaaaa actaagtaga ggtcaattaa aaatattctt
     9541 tggttatgca gctggagtag gaaaaaccta ctctatgttg gaatctgcac aaaatttaaa
     9601 aaaagtgggt gtagatgtag tagttggata tattgaacca cacactagac cagaaacatt
     9661 ggctttactt gatggcttgg aaattcttcc tgtaaaggag atagaatata aatctataaa
     9721 actaaaagaa tttgatttag atgctgcttt agcaagaaaa ccagaagtta tattagtaga
     9781 tgaatttgcc cattctaatg ttgctgggtt aaggcataca aaaagatggc aagatataga
     9841 agaattgctg ttagcaggca taaatgtcta cactactgta aatgtacaac atttagaaag
     9901 tctaaatgac attgttgaga taattacaaa cgtatcagtt aaagagacta taccagataa
     9961 gtttttggat acaaatacac agttagaact tatagatgtt gaacctgatg tacttcttga
    10021 aaggttcaat gaaggtaaga tatataagaa agaacaagct actagagcaa aaaataattt
    10081 ttttataaaa gataacctgg ttgcacttag agaaattgca cttagaaaaa cagcagagag
    10141 agttaataaa gaagttcaaa tgaccagact atcaaagggt gatgtaactg taatacctac
    10201 atcagataca cttcttgcat gtataagtcc atctccatct tcagcaaaag ttattagaac
    10261 tgcatccaga atatcagaca gtacatttgc aaaatggata gctttatatg ttgaaactcc
    10321 aaacacagca agacttccat ttgatgaaca aaaacaacta caaaataacc taaaattagc
    10381 taagaaatta ggtggagaaa taattgtttt acatggtgaa aatataatag agcaaatact
    10441 tagaattgca aagctgagaa atgtaacaaa aattgtaata ggtagaaatc attcaagtaa
    10501 taaaagattt agtaaaagat ttaaaaaaga tatagttgac aaattaattg atgaagttga
    10561 ttatatagat atacatataa ttccatataa atttgcctca gatgttaaat acaaacctaa
    10621 aaaggataaa tcatcaataa aatctaaatt taaaattagc aaagttgatt ttataaaact
    10681 tatatttatt actttgatga taagtatttt agcatatgca gtacaaagca tgggatttat
    10741 aagagaaaat atattgttaa tatatatgtt gggtgtagtt ttagtttcta tgtggactaa
    10801 agggtatagt acaggtataa taagttctgt atttaatata gttcttttaa attatttttt
    10861 tacagcacca ttatatacat tatctatagc agattcaaac tatatagtaa cactggttgt
    10921 attctcaata gtaggtataa taactagcac tcttacgtct aagatacagc atgaagcaga
    10981 aactgctgct aaaagagaag aaaatacaaa aatgatatat caaataatta agggattttt
    11041 aagattatca aataaagatg atatagtaaa caaaggtata gaacttttgt ctcttagctt
    11101 aagtagagat gtaatttgtt atttaaatgt agataagcat aataaatcaa agctttacaa
    11161 aaaaaatact aataataagg ataaaaatga tttggatagt gaagatgaaa aagctgttgc
    11221 aagttgggtt ttatccaatt caacagtagc aggaaatgac acggatacac ttcctggttc
    11281 aaagggatac tatataccaa ttgtaggaat gaatttaact ttaggagtag tgggaatatc
    11341 atgtattgat tcaaaattgg atacagaaga tataagctta attgaaacaa taattgctca
    11401 aatggcaata gcgcttgata gagaaatctt gtcagaagct aaagaaaata ccaatcttga
    11461 gattgaaagg gaaagattaa gaagtaatct tttaagagca gtttcgcatg accttagaac
    11521 tccactagca ggaatatctg gtgcagtaag tacaattata aaaaacaagg gtactatagg
    11581 tcaagatata atagatgaat tacttagtgg tgtatatgaa gatactcagt ggttaataag
    11641 acttgttgaa aatttactta gtatgaccaa gatagatgaa gggaaattgg aagttaaaaa
    11701 acattctgaa ctggttgagg aaattatatc agaagcgtta caaaaaataa aaaaaagaat
    11761 tgaaaatgca cttatagata ttaatatacc agaacagata ttatttgttc ctatggatgc
    11821 aaaattaata gaacaagttt taattaattt aatagataat tctttgaaat attcaaagga
    11881 agattgtaag atagagataa atgtatatga gaaagatgat tatgtttggt ttgaagtatc
    11941 tgataatggt cctggtatat caaaggaact taaaaagcat atctttgaca gattttttac
    12001 tggagaagaa ggagctaaag attccagaaa gggtgttgga cttggattat caatttgtaa
    12061 atctataatt caagctcata aaggtgaaat tatggtagaa aacaataaag ataagggttc
    12121 aacttttaag tttagtttgc ctaaggaaaa tgaatagttt taaattttaa aattagtttt
    12181 ggaggaagaa atgagtaaaa aacttatatt gttagtagag gatgataaga caataagaaa
    12241 atttatatct actgcacttt taacacagga ttatgatgtc aaggaggcta taactggaaa
    12301 agaaggtata tctatagctg tctcatattc tccagatgtt gttcttttag atttaggctt
    12361 agaagatatg gatggaatag aggtaataaa agctatcaga caattttcaa atataccaat
    12421 aattgtagtt tcagctagag agcaagatag agacaaggta gaagtatttg atgcaggggc
    12481 agatgattac ttaacaaaac cattctccat agtagagtta ttagcaagag taagagtagc
    12541 atttagacat tcacaagtag aagctcaaca aaaagatgat gttaaatcta cgtttgaagt
    12601 agataaatta ttgattgatt ttgataaaag aaaagttatt gttgatgatg ttgaagtcca
    12661 tttgacacct attgaatata atattctttc attacttgca aagtatcatg gaaaagtgct
    12721 tactcataat tttataataa aagaaatatg gggtagtgtt atagggaatg agactaagtc
    12781 tttaagggtc tttatggcaa cgttaagacg aaaaatagaa aaacaacctg caaacccaag
    12841 atatataatt acagaagttg gtgtaggata cagattaaat gatgaatagt tgtaatatat
    12901 taattattag attatgtgct tattaatata tattaaaatt atatagttta gttaaaataa
    12961 gaatgaagtg tatgtgcttg ttgtcagttt agcacatgca ttttattttt tagctttttt
    13021 agtatcaata attttttagt atcaataata tgaaatatgt tttaaaataa gtatatagtg
    13081 taatatgtaa taaaaacttt ggttaaataa taaatgt
//
LOCUS       DAMJVK010000103        13053 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.107, whole genome shotgun sequence.
ACCESSION   DAMJVK010000103 DAMJVK010000000
VERSION     DAMJVK010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13053
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.107"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            129..1010
                     /locus_tag="PC414_003101"
     CDS             129..1010
                     /locus_tag="PC414_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2465480.1"
                     /translation="MNLYHLRYFVTLAHLEHYTKAAENLSITQPSLSHAISLLENELG
                     VALFEKEGRNIVLTKYGKIFLKDVEKSLEILDSSVKSLKITGTGEGQIDLAFLRTLGT
                     DFIPDIVHKFLKSNPAKSIDFKFHTGVTTDIIQGLKERKYDIAFCSKLEKEKGIEFIP
                     VAKQDLVLIVPYSHPLAAKDTIDLKETIPYPQIVFNQRSGLRYIIDDMFKKINQQPNI
                     VYEVEEDQVIAGLVAKNFGIAVVPNMNMLSFTKVKVIQIIHPSWERNFYLAFIKDRYL
                     PPAIKNFKNFVIKNAQL"
     gene            complement(1218..2081)
                     /locus_tag="PC414_003102"
     CDS             complement(1218..2081)
                     /locus_tag="PC414_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HDF2465481.1"
                     /translation="MLSSYVKRVSKIIKKKYIMFIDESGRADINHEDPFTLTGVIFEY
                     KYAVSQGDYVCSLRKEMDAFKEYCFGTSNIGIHLTDISRKSRAFEKFDDSQIKLFYDE
                     LPLFLSRLECTIISVTVDKARLKKYYAPSKEPYVVAFIHVLQNFYSFVNNETVESARI
                     VIESRDDVSNLKVQKAFFDVFNNGTIYLDIDEELRDKVKGFIIAKKEDADYREGLEIA
                     DILCNPLSRARRGLIEANPKCMQYGEKNKIFKSVRNKIYTPTSVNDIRNWGFKEVPIV
                     EPVGPWLEKMR"
     gene            complement(2236..4170)
                     /locus_tag="PC414_003103"
     CDS             complement(2236..4170)
                     /locus_tag="PC414_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp22"
                     /protein_id="HDF2465482.1"
                     /translation="MKKSLKKYLVLALTLVLVFACGVPESSAASKHVIIVNSRKNTLG
                     YFVNNKLVKEFRVATGKKGSETPTGKTKVVNKIKNRPYYKGNIPGGSPRNPLGDRWMG
                     LALKGTYGDTYGIHGNNNESSIGKHISGGCIRMHNKDVRWLFDQVPVGSDVIIDYSND
                     SYVKIAAKYKINLNQTGWKTENGKKYYVKSDGTYQKNSWLKVNGKMYYFDASGVMQTG
                     WKTINNKKYYLGTDGARVSGWKVIDGKTYYFNSDGVMQTGWQEKNGKKYYLGSDGLAV
                     TGWQEIDGNKYYFDKTGIMQTGWQQIDGKSYYLDKDGKMLTGSQKIDGKDYTFNEDGT
                     INPTWDTIIGANRFDTAKKISSVGNWNADSSDTVILVNGNAIADGITATPLASSYDST
                     ILLTNTANLPTETVEEMKLLAPKTVILIGGENAISSKLEQEIKTTFNTETKRIAGQDR
                     YQTATRIAEELGNREEIKTAYMVSGNGEADALSVASKAGEEKQPIILVNKDGITEESY
                     KWLTERKLENAYFIGGPSAINDSVIAKMNDITTEDISGNRIYGDSRVDTNAKVIEKFY
                     GDTDLQAVLVSKSDALVDALSAGPLAVKLHSPIVLMDNSGLSSEQQRVFANKKVETPY
                     QIGGGVSYIVMDKLMDILAK"
     gene            complement(4282..5295)
                     /gene="galE"
                     /locus_tag="PC414_003104"
     CDS             complement(4282..5295)
                     /gene="galE"
                     /locus_tag="PC414_003104"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HDF2465483.1"
                     /translation="MAVLVAGGAGYIGSHTAIELLESGYEVVIVDNLSNSNSIVVDRI
                     KELSKKPVKFYNIDIRNKDEMHVVFKENNIESIIHFAALKAVGESVEKPIEYYSNNLI
                     STLNLFELMREYGVKKFVFSSSATVYGDPHTCPILEDFPLSVTNPYGRTKLMIEQMLV
                     DISKADKSLDIALLRYFNPVGAHKSGRIGEEPNGVPSNLMPYITKIAVGKLKELSVYG
                     NDYPTHDGTGVRDYIHVLDLAAGHVKALQKLEENPGLVVYNLGTGKGYSVLDLVKAFS
                     KASGKEIPYKIVGRRAGDVAMCYADSSKAEKELGWKAKYELEEMCEDSWRWQSMNPNG
                     YEE"
     gene            complement(5319..6188)
                     /gene="galU"
                     /locus_tag="PC414_003105"
     CDS             complement(5319..6188)
                     /gene="galU"
                     /locus_tag="PC414_003105"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDF2465484.1"
                     /translation="MTVKKAIIPAAGLGTRFLPATKSQPKEMLPIVDKPTLQYIIEEA
                     IESGIEEILIITGRNKKSIEDHFDKSVELELELEQKGKTEMLEMVRDISNMVNIHYIR
                     QKEPKGLGHAIYCAKSFIGDEPFAVLLGDDIVDSDVPCLKQLIDTYNEYKTTVLGVQK
                     IAKEDTNKYGILDVKHIEDRVYKVKDMVEKPAIEEAPSDIAILGRYIITPAIFSILEK
                     QTPGKGGEIQLTDALQTLGKQEAIYAYNFEGRRYDVGDKIGFLEATIDFALKRENLKD
                     DLMDYMRKKVSEK"
     gene            complement(6386..6706)
                     /locus_tag="PC414_003106"
     CDS             complement(6386..6706)
                     /locus_tag="PC414_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465485.1"
                     /translation="MSSKYNVWTFNYEFLGLDSGDEKNNEVYYEIDLDEILNENYIDE
                     DIDSGLDSDGNSILGELISEMDIDEDVSVDMTYQELEVDLEDLDSYLDEDIDSDIKGI
                     LDEI"
     gene            complement(6696..8687)
                     /locus_tag="PC414_003107"
     CDS             complement(6696..8687)
                     /locus_tag="PC414_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465486.1"
                     /translation="MLKLGEKIIYELSDGFELSGISRYSKEYKKLHITNLGNIIISKS
                     DEVNEDTGVKYIYSFSDNKEKLEAVLEDSQIIVYGNNLPFYIKSENSSKNVTANLTMK
                     ITLDEYDLICKSQREFIFYLKDNMTILSLDDNKFYMGGINKDNEKFIFISGKNRFEIN
                     FDDIERYILEDKRVSLKGYFHMEREGIIVRSVSIFNNNIDSVVPSDLNERVKDNQKIG
                     NLPKDCEIVFCKISGNIDGFDYKNTNMLLVKYQDQLIFINKKSKKTIVKSAKDNCSKL
                     NLGEDIILYDNKNVFNLHINDKNREIMQIDDLKDIENEIVGYTLKHAPFFIQEDFDSL
                     TILKSFQKEIISIKNSDIKDIVINKELENENSNFVETEIKFNNQKVLLNLSKSMVQKL
                     MQDVFIYAKQPLLKENSIEVIYKNWSKAMNDMIIFNFFGNIYYMKSEFDKILEKELND
                     EIRIEVINSLYKQIQEQRNNLDLLSAYMPRILENQEIDLFEKYNTKLDVQVFKQIKNL
                     LSDLSYNISSYLNEVEKSLDNIIFVISGEDKKKYNYRMLKESESASLDVFLKQAISRL
                     NHLVENMYPYYVDETSREMFKLFELLWKNYRNIDDDSIKEILFERITNTYVFKQLTLN
                     NSTKERRKDIIEKIYNSVDYGTNKLDENMFFTGGIKYVK"
     gene            complement(8810..11353)
                     /locus_tag="PC414_003108"
     CDS             complement(8810..11353)
                     /locus_tag="PC414_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HDF2465487.1"
                     /translation="MKVLSENNIIRNTVLNVTASFLKQESKINEKLDGVLEKKFEKVE
                     FNEAKYAELLKFNILFYKTLARNTEPLIGKWIVDKYIPEIDELEKDLELTTAKCRKYV
                     NKAMKEGLDSLKANDLNSFLAYDKMELSERRRRLEKDYKVLNLYKDLLNISLRKISLE
                     KKECGDLFLKNQADAKRELKREIIFCVNKILASNKDVVPNNVEENNEQNKVEIKATEI
                     EDLQLGKDNSENDMLLNEIKNSKVKHESKSNDIESKFIDKLNSLNNESISQTIHNEYK
                     KLCGLEELHSVEAYGFGKEIIKDFACATVVLEFLKRRNRDLIEGAMRLTIIGEFGPEN
                     FKEFVDYVIKNKTEISEDTWNKAQNLIKDNYSELENHEIASKRTRRNKDIDIEEYIYM
                     IKNADKDICFRSSISIEDDAEEGTKEEVDSNNQDIGDVVEDKDTTDKEYDSNKEDIIE
                     PENKKSKKKAKLFGFIKKDNEEAEQEEENLNDISPDIILDKPVENHQVKSEEIEQNEL
                     KEIKQEEPSQHIEEERSVKIEKPINNNLDEKVSSNNESKLEKESKNLEDKKAKEIKEE
                     KLENEKSVVIPIKKKENSNKKSKNSSKDKYRENKKEMKNYVSDKEDSLDDEEVVSKKS
                     RLKETIIAVVIVVIVGVGYFITVGNNKKNDKENIPKSSTQQQANNKLTEEEKKAQAEK
                     EKKEAEEKAKAEKEAQEKAQAEKKAKEMEAYKDGKGVYYTVYAGSLKVEKTAKETAKE
                     YEAKGISSTIIQENGYYKIKIGDYSQYGEAQEKCNELAKKSIDTYIAMYDKYYDYKLE
                     ELKESAPSLSAEELKQKYEDLRSELKNKSGYREYVKHLDKLYEEIVEGA"
     gene            complement(11631..11965)
                     /gene="rnpB"
                     /locus_tag="PC414_003109"
     ncRNA           complement(11631..11965)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PC414_003109"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            12097..12774
                     /gene="srtB"
                     /locus_tag="PC414_003110"
     CDS             12097..12774
                     /gene="srtB"
                     /locus_tag="PC414_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtB"
                     /protein_id="HDF2465488.1"
                     /translation="MKKLYRIVINIILVLVILYSGFNIYSKLTKYNHDTKISSELQKK
                     EYKKEDLSKINSDFKFWLSVENTNINYPVVQSKDNSYYLDKDFYKKDSISGTLFMDYR
                     NKSIDDKNIIIYGHNMKNKTMFNNLNKFKDADFFKKNNKIKITLNGKEFLYDVFSAYI
                     VESDYDYLKTNFNNESDYQNYINDITSKSLYKSPIKVNSNDKIVTLSTCTYEFDDARM
                     VIHGRLI"
     gene            complement(12934..13008)
                     /locus_tag="PC414_003111"
     tRNA            complement(12934..13008)
                     /locus_tag="PC414_003111"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12974..12976),aa:Thr,seq:tgt)
BASE COUNT         4019 a         2291 c         1332 g         5411 t
ORIGIN      
        1 aacgataaat ttttttaaaa gcttttattc ttactctata atcatatata ataaaaatgt
       61 aaaaatatct taaaggttaa cttctacaaa tatataaata aaaataccta tgaaaggatg
      121 aacaaaaatt gaatttatat cacttacgct attttgttac attggcacat ttagaacact
      181 atacaaaagc ggcagaaaat ttatctataa cacaaccaag cttaagtcat gctatttctt
      241 tactagaaaa tgaacttggg gtggctctat ttgaaaaaga aggtagaaat atagtcctta
      301 ctaagtatgg aaaaatattt ttaaaagatg tggaaaaatc attagaaatt ttagattcaa
      361 gtgtaaaatc attaaaaata actggtactg gtgaaggaca aatagactta gcttttctta
      421 gaacacttgg tactgatttt atcccagaca tagtgcacaa atttttaaaa tctaatcctg
      481 ctaaatcaat agattttaaa tttcatactg gtgtaactac tgacattatt caaggtctta
      541 aagaaagaaa atatgatatt gcattttgtt cgaaacttga aaaagaaaag ggaattgaat
      601 ttatacctgt tgccaaacaa gatttagtac taattgtacc atatagtcat ccactagctg
      661 caaaggatac tatagattta aaagaaacca taccttatcc tcaaatagta tttaatcaga
      721 gaagtggtct tagatatata attgatgata tgtttaaaaa aataaatcaa cagcctaata
      781 tagtctatga agtagaggaa gaccaagtaa ttgctggatt agttgcaaaa aatttcggaa
      841 ttgctgtagt cccaaatatg aatatgctta gctttacaaa agtaaaagta attcagatta
      901 ttcacccaag ttgggaaaga aacttttact tagcatttat aaaagataga tatttgcctc
      961 ctgcaattaa aaactttaaa aactttgtca ttaaaaacgc tcaattataa tttattaata
     1021 aaggggttat ttttaaatat cctcttttat tctttttata aaataaaaaa atctatactt
     1081 ttaaaagtat agattcctaa ctgacagtcg gcatccgagt ctcagtaatt atcgttatac
     1141 aacagcaact ggattcagtt cctgaataac taggttactt acattatatc agatttttta
     1201 aaatttgtca attgtatcta acgcattttt tcaagccatg gtccaacagg ctctacaata
     1261 ggaacttctt taaatcccca atttcttata tcattcacac tcgttggagt atatattttg
     1321 tttctgacag atttgaatat tttgtttttt tctccatatt gcatacactt tggattagct
     1381 tctattaacc ctcttcttgc tctgctaaga ggattacata aaatatctgc tatttctaaa
     1441 ccttctctat agtcagcatc ttcttttttg gcaattataa aacccttaac cttatctctt
     1501 aattcttcat ctatatctaa gtatattgtt ccattattaa atacatcaaa aaacgccttt
     1561 tgtactttta agtttgagac atcatccctt gactctataa ctattcgtgc agattctaca
     1621 gtttcattat ttacaaatga ataaaaattc tgaagaacat gtataaaagc cacaacatac
     1681 ggttcctttg aaggtgcata atatttcttt aatctagctt tatctactgt tactgatatt
     1741 atagtacact ctaatctaga tagaaataaa ggcaactcat catagaataa cttaatctgt
     1801 gaatcatcaa acttctcaaa tgctcttgat tttcttgata tatctgtcaa atgtattcct
     1861 atgttactgg ttccaaaaca atactcttta aatgcatcca tttctttcct caaagaacat
     1921 acataatcgc cttgacttac tgcatattta tattcaaaaa ttactcctgt caatgtaaat
     1981 gggtcttcat gatttatatc agctcttcca ctttcatcaa taaacattat atattttttc
     2041 tttataatct tactcaccct cttaacatat gatgatagca tctatattca ttttatatga
     2101 gttttaaaaa tattccaaga tttttatata acttaaactt aattttatac taaaaaattg
     2161 agatgtaaaa caatcaagct tttattgatt gaatttcaca tctcaattat aattattttt
     2221 caatatatat ttttactatt ttgcaagaat atccattaat ttatccatta ctatataaga
     2281 tactccgcct cctatttgat aaggagtttc aactttttta tttgcaaata ctctttgttg
     2341 ctctgatgat aatcctgaat tatccattaa aactattggt gaatgtaatt ttacagctaa
     2401 tggtccagct gataaagcat ctactaaagc atctgattta ctaactaata cagcctgtaa
     2461 atcagtatct ccatagaatt tttctatcac tttggcatta gtatctactc tactatctcc
     2521 atatattcta tttcctgaaa tatcttcagt agttatatca ttcatttttg ctattactga
     2581 atcgttaatt gcacttggtc cacctataaa gtatgcattt tctaattttc tttctgttag
     2641 ccatttataa gattcttctg taattccatc tttattgact aatattatag gttgtttttc
     2701 ttccccagct ttagaagcta cagataaagc atctgcctcc ccatttccag aaaccatata
     2761 tgcagttttt atttcttctc gatttccaag ttcttcagct attcttgtag cagtttgata
     2821 tctatcttga cctgctattc tctttgtttc tgtgttgaaa gtagttttta tttcttgctc
     2881 taatttagaa cttattgcat tttctccacc tatcaagatt actgttttag gtgctaataa
     2941 cttcatttct tcaacagttt cagttggtaa attagctgta ttagtaagta gtattgttga
     3001 gtcatagcta cttgctaaag gagttgctgt tataccatca gctattgcat taccatttac
     3061 taatattaca gtatctgaac tatcagcatt ccagtttcca actgaactta tctttttagc
     3121 tgtatcaaat ctatttgcac ctataattgt atcccatgta ggatttatag ttccatcttc
     3181 attaaaagta taatctttcc catctatttt ttgagaacca gttagcatct ttccatcttt
     3241 gtctaaatag taagacttac catctatttg ttgccatcct gtctgcatta ttcctgtttt
     3301 gtcaaagtaa tacttattac catctatttc ttgccatcct gttactgcta aaccatcaga
     3361 tcctagatag tatttctttc cattcttttc ttgccatcct gtttgcatta ctccatctga
     3421 gttaaagtaa taagtttttc cgtctattac tttccatcca gatactcttg ctccatcagt
     3481 tcctaaataa tattttttgt tattaattgt tttccatcct gtttgcatta ctcctgaagc
     3541 atcaaagtaa tacatttttc catttacttt tagccaacta ttcttttggt atgttccatc
     3601 agattttaca taatacttct taccattttc tgttttccat cctgtttgat ttagatttat
     3661 cttatatttt gccgctatct ttacatagct gtcgttacta taatctataa ttacatctga
     3721 acctacagga acttggtcaa ataaccatct aacatcttta ttgtgcattc ttatacatcc
     3781 accagaaata tgctttccta tagaactctc attattattt ccatgtattc catatgtatc
     3841 tccataagtt ccttttaatg ctaatcccat ccatctatct cctaatggat ttcttggact
     3901 tcctccaggt atattacctt tataatatgg tctatttttt atcttgttaa ctacttttgt
     3961 tttccctgtt ggtgtttctg accccttttt acctgttgca actctaaatt cttttacaag
     4021 tttattattt acaaaatatc ctagagtatt ttttcttgaa tttactataa tcacatgttt
     4081 actggctgcg cttgattctg gtacgccaca tgcaaatact aaaactaaag tcagtgctag
     4141 tactaaatac ttttttagac tttttttcac tataacttcc ccctctttta gaaacattac
     4201 tacatgtgtc taattataac ataaaattac caatacctta tatttacaaa tttgttacat
     4261 ttatacatat aagtatgtat tctactcttc atacccatta ggattcatac tttgccatct
     4321 ccatgaatct tcacacatct cttcaagttc atacttagct ttccatccaa gctctttttc
     4381 tgcctttgaa gaatctgcat aacacatggc tacatctcca gcccttcttc ctacaatctt
     4441 atatggtatt tcttttccac ttgctttaga aaatgctttt actaaatcaa gaacactata
     4501 cccctttcca gttcctaaat tataaacaac aagtcctgga ttttcttcta atttttgtaa
     4561 tgcttttaca tgaccagcag ctaaatctaa tacatgtata tagtctctaa ctccagttcc
     4621 atcatgtgtt ggatagtcat taccataaac actcaattcc ttcaatttac ctactgctat
     4681 ttttgttata taaggcatta gattgctcgg aactccattt ggttcttcac ctattctacc
     4741 acttttatga gcaccgactg gattaaagta tcttaaaaga gctatatcca atgacttatc
     4801 tgcttttgat atatctacta acatctgctc tatcataagt ttagttcttc catatggatt
     4861 ggttacagaa agtggaaaat cctctaatat aggacaagta tgtgggtctc catatacagt
     4921 agctgaagaa ctaaatacaa actttttaac accatattct ctcattaatt caaataaatt
     4981 taaggtactt attagattat tgctgtaata ttcaattggt ttttctactg attcaccaac
     5041 tgcttttaaa gctgcaaagt gtataattga ttcaatatta ttttctttaa atacaacatg
     5101 catttcatct ttatttctga tatcaatatt ataaaatttt actggttttt tagataattc
     5161 cttgattcta tcaaccacta ttgagttgct attacttaag ttatcaacta taacaacttc
     5221 ataacctgat tctaaaagtt ctattgctgt atggcttcct atatatcctg ctcctccagc
     5281 tactaaaact gccattgtaa ttacctcttt tctaatattt atttttcact tacttttttt
     5341 ctcatatagt ccattaaatc atcttttaaa ttttctctct ttagagcaaa gtctattgtt
     5401 gcctctaaaa aaccaatttt atctcccaca tcatatcttc taccttcaaa gttataagca
     5461 taaatagcct cttgttttcc aagagtttga agtgcatctg tcagttgtat ctctccacct
     5521 ttaccaggtg tctgtttctc taatatgcta aatattgcag gagttattat atatcttcca
     5581 agtatagcta tatcagatgg agcttcttca attgcaggtt tttcaaccat atctttaact
     5641 ttatatactc tatcttcgat atgttttaca tccaatatac catatttatt agtatcttct
     5701 ttagctatct tttgaacacc taatacagtt gttttatact cattataagt atctattaat
     5761 tgttttaagc acggaacatc tgaatctact atatcatcac ctaataatac ggcaaaaggt
     5821 tcatcaccta taaaactttt tgcacagtaa attgcatgtc caagaccttt tggctctttt
     5881 tgtcttatat aatgaatatt taccatattt gatatgtctc ttaccatttc taacatctct
     5941 gtcttacctt tttgctctag ttctaactct aattcaactg atttatcaaa atgatcttct
     6001 atagattttt tatttctacc tgttattata agaatttctt caattcccga ttctatagct
     6061 tcttctatta tatactgtaa agttggttta tctactatag gaagcatttc tttgggctgt
     6121 gattttgttg ctggtaaaaa tctagttcct agccctgctg ctggtattat tgctttttta
     6181 actgtcatct gcattttttc acccttcttc gtaaattttc tattgtacca aattaatata
     6241 ccatatttat actttttctt caaactctat attatcccaa tatttaatgc tataagataa
     6301 tataaggtag atgaaatttc atctaccttt agttttataa gttaatattg tattgtttat
     6361 attttatcca aaacttatac aggtactaaa tttcatctaa gatgcctttt atatcactat
     6421 ctatatcttc atctagataa gaatctaaat cttctaagtc aacttctagt tcttgatatg
     6481 tcatatcaac actgacatcc tcatctatat ccatttctga tattaattct cctaaaatac
     6541 tatttccatc agaatctaaa ccactatcta tatcttcatc tatatagttt tcatttaaaa
     6601 tttcatctaa atcaatctcg taataaactt cattattttt ttcatcccct gagtccaatc
     6661 ctaagaactc atagttaaaa gtccaaacat tgtatttact tgacatactt tattcctcct
     6721 gtgaaaaaca tattttcatc tagtttattg gttccataat caacagagtt atatattttt
     6781 tcaattatgt cttttcttct ttcttttgta ctattattta atgtcaattg tttaaataca
     6841 tatgtatttg ttattctctc gaataatatt tcttttatac tatcatcatc aatatttcta
     6901 tagtttttcc acaataactc aaataactta aacatttccc tactagtttc atctacataa
     6961 taaggataca tattttctac taaatggttt aatcttgata tggcttgttt taaaaataca
     7021 tcaagacttg cactttcact ttcttttaac attctataat tgtatttctt cttatcttct
     7081 cctgaaatta caaagatgat attgtctaaa cttttttcaa cttcatttaa ataactgctt
     7141 atattatatg ataaatcaga caatagattt ttaatctgct taaacacttg tacatcaagt
     7201 ttagtattat acttttcaaa caaatctatt tcttgatttt ctaatattct tggcatatat
     7261 gctgacaata aatctaaatt gtttctttgt tcttgtattt gcttataaag tgaatttata
     7321 acttctattc ttatttcatc atttaactct ttttctaaaa tcttatcaaa ttcagatttc
     7381 atatagtaaa tatttccaaa gaaattaaat attatcatat cattcatagc tttagaccaa
     7441 ttcttatata tcacttcaat agaattttct ttaagtagag gttgctttgc atatataaat
     7501 acatcttgca taagtttttg aaccatactt ttacttaagt ttaaaagtac tttttgatta
     7561 ttaaatttta tttccgtttc tacaaaatta gaattttcgt tttcaagttc tttattaatt
     7621 acaatgtctt ttatatcact attttttata gatatgattt ctttttgaaa agactttaga
     7681 atagtcaagc tatcgaaatc ttcttgaata aaaaatggag catgttttaa tgtatatccc
     7741 actatttcat tttctatatc ttttaggtca tctatttgca taatttctct atttttatca
     7801 tttatatgta ggttaaatac atttttatta tcatataata ttatatcttc tcctaaattt
     7861 aacttagaac aattatcttt cgctgacttt acaatagtct tcttagattt cttatttata
     7921 aaaattaatt ggtcttgata ttttactaaa agcatgtttg tgtttttata atcaaatcca
     7981 tctatatttc ctgatatctt gcagaatact atttcacaat ctttaggaag atttcctatt
     8041 ttttggttat ctttaactct ctcatttaaa tcagatggga ctacactatc tatattgtta
     8101 ttaaatatag atacagacct tactataatc ccctctcttt ccatatgaaa gtatcccttc
     8161 aatgacactc ttttatcttc taaaatgtat ctttctatat catcaaaatt tatttcaaat
     8221 ctatttttcc cactgataaa tataaacttc tcattatctt tattaattcc acccatataa
     8281 aacttattgt catctaaact taatatagtc atattatcct ttagatagaa tataaattct
     8341 ctttgacttt tgcagattaa atcatattca tccaaagtta ttttcatagt caaatttgct
     8401 gtaacatttt tacttgaatt ttcacttttt atgtaaaaag gcaagttatt tccatagaca
     8461 attatttgag aatcttctaa aactgcctct aatttttctt tattgtctga aaaactatat
     8521 atatatttta cacctgtatc ctcattaact tcatctgatt tgctgattat tatattccct
     8581 agatttgtta tatgaagctt tttatattct tttgagtatc tacttatgcc actaagctca
     8641 aacccatctg aaagctcata tatgattttt tctcctaatt tcaacatacc ttcaccacct
     8701 aaacagaatt ataagtattt tataaaggtg aataaaatgt ctcaaaacaa ataatatttt
     8761 ttttaatact ttttattctg agacatagtt tctccttttt tataatgctc taagcacctt
     8821 caactatttc ttcataaagt ttatctaaat gtttaacata ttcacgatat ccagatttgt
     8881 tctttaactc acttcttaaa tcttcatatt tttgtttaag ttcttcagca ctcaaagaag
     8941 gagctgattc ttttaattct tctaacttat aatcatagta cttatcatac atagctatat
     9001 atgtatcaat agacttctta gctagttcat tacatttttc ttgtgcttct ccatattgag
     9061 aataatcacc tatttttatt ttatagtatc cattttcttg aattattgtt gaagaaatac
     9121 ctttagcttc atattctttt gctgtttctt ttgctgtttt ttcaacttta agtgaacctg
     9181 cataaacagt gtaatataca cctttaccat ctttatatgc ttccatttct tttgctttct
     9241 tttcagcttg tgctttttct tgagcctctt tttcagcttt tgccttttct tctgcttctt
     9301 ttttctcttt ttcagcttgt gcttttttct cttcttcagt taatttattg ttagcttgtt
     9361 gttgagttga tgactttgga atattctctt tatcattttt cttattgttg cctactgtta
     9421 taaaatatcc tacaccaaca ataaccacta taactactgc tataatagtt tcttttaatc
     9481 tactcttctt agaaacaacc tcttcatcat ctaaagaatc ttctttatct gatacataat
     9541 ttttcatttc tttcttgttt tctctatatt tgtccttaga agaattttta gattttttat
     9601 tactattttc tttcttttta ataggtatta ctactgattt ttcattttct aatttttctt
     9661 cttttatttc ttttgctttt ttatcttcta agtttttact ttctttttca agcttcgatt
     9721 cattatttga agatactttt tcatctaagt tattatttat tggtttttct atttttacac
     9781 tacgttcttc ttctatatgt tgacttggtt cttcttgttt tatttctttc aattcatttt
     9841 gctctatttc ttctgatttc acttgatgat tttcaactgg tttatctaaa attatatctg
     9901 gagaaatatc atttaaattc tcttcttctt gttcagcttc ttcattgtct tttttaataa
     9961 atccaaataa tttagccttc tttttactct ttttattttc tggttcaatt atatcttctt
    10021 tattgctgtc gtactcttta tctgtagtat ctttatcttc tacaacgtca cctatatctt
    10081 gattattact atctacttct tctttagtcc cttcttcagc atcatcttct attgaaatac
    10141 tacttctaaa gcaaatatct ttatctgcat tttttatcat gtaaatgtat tcttcaatat
    10201 cgatatcttt atttcttcta gttctttttg atgcaatttc atgattttct aattcactat
    10261 agttatcttt tattagattc tgtgctttat tccatgtatc ttcactaatt tctgttttat
    10321 ttttaattac atagtccaca aattccttaa aattttcagg accaaattct ccaattatag
    10381 tcagtctcat tgcaccttct ataaggtctc tatttcttct ctttaaaaat tctaatacaa
    10441 ctgttgcaca agcaaaatct ttaataattt ctttgccaaa accatatgct tcaacagaat
    10501 gaagttcttc taatccacat aacttcttat attcattgtg tattgtttga gatatacttt
    10561 cattatttaa gctatttaac ttatctataa acttcgattc tatatcattt gattttgatt
    10621 catgttttac ttttgaattt ttaatttcat ttagtagcat gtcattttct gaattatctt
    10681 tacctaattg caaatcctca atttcagttg cttttatttc tactttgttt tgttcgttat
    10741 tctcttccac attattaggt accacatctt tattactagc taatattttg tttacacaga
    10801 atataatttc tctttttaat tctcttttcg catctgcttg atttttaaga aataaatctc
    10861 cacattcttt cttttctaaa cttatttttc taagagatat attaagaagg tctttatata
    10921 ggtttaaaac cttataatcc ttttctaaac gtcttcttct ctctgatagt tccattttat
    10981 cataagctaa aaaactattt aaatcattgg cttttagaga gtctaatccc tctttcatag
    11041 ccttatttac atactttcta cattttgctg tagttaactc taaatctttt tctaattcat
    11101 ctatttcagg tatgtactta tctactatcc atttgccaat aagtggttct gtatttcttg
    11161 ctaatgtctt ataaaacaat atattaaatt ttaaaagctc tgcatatttt gcttcattaa
    11221 attctacttt ttcaaatttc ttttccaaaa caccatctag cttttcattt attttagact
    11281 cttgttttaa aaaacttgct gtgacattta aaacagtgtt tcttattata ttattttcac
    11341 tcaaaacctt cactccttaa atcaattatt ttattgtaat atttacattg tgcaaagttg
    11401 ctaatttgat tatgatatct aaataattat ttactaaatt tgctacatct tatatcatat
    11461 catattattt acatcatttt actttttctt tttatatcta tcttatatta taccattttt
    11521 aatatctgta ctaacttata ttacaatatt cctcaaaatt ttaaaagtag attagacaaa
    11581 tttacaaata aaaaaacatc agtttaaact gatgtttttg actatcgaaa taattaagca
    11641 agtctataag cggagttctg tattagataa tcatctttct aggcctattg tcaccaatag
    11701 gctcaagcga cctacctacc ggagagtacg agcagcactc tttattccta tcttggtctt
    11761 gctccaggtg gggtttacat agccaactag tcgcctagct gctggtgagc tcttacctcg
    11821 cctttccatc cttaccacaa tagtggcggt ttatttctgt tgcactatcc ttaggatcgc
    11881 tcccactagg cgttacctag caccctgctc tatggagctc cgactttcct catgtaagat
    11941 tacatgcgat tatctggctt acttaatatt tattttactt agttatgtta acacaaattc
    12001 ttttacatgt caaagtaata ttatactaaa aatttaaatt tattcaaaaa tagtgtaata
    12061 tagatatata aactacattt aactcgaggt gatctattga aaaaattata tagaatagta
    12121 attaacatta tactagtatt agtaatactt tacagtggat ttaatatata ctcaaaactt
    12181 accaaataca atcatgatac taaaatatcc tcagaacttc aaaaaaaaga atataaaaaa
    12241 gaggatttat caaagattaa cagtgatttt aaattttggc tttctgttga gaatacaaat
    12301 ataaattatc cagttgtaca atctaaagat aattcttact atcttgacaa agatttttat
    12361 aaaaaagatt ctatttctgg tacactgttt atggattata gaaataaatc tattgatgac
    12421 aaaaatatca taatatatgg acataatatg aaaaataaaa ctatgttcaa caacctaaat
    12481 aagtttaaag atgctgactt ctttaaaaaa aataataaaa taaaaataac cttgaatgga
    12541 aaagaatttt tatatgatgt tttttctgct tatattgtag aatctgatta tgattatcta
    12601 aaaacaaatt ttaacaatga atctgattat caaaattaca taaatgatat tacttctaaa
    12661 tcattgtata aatctcctat taaggtaaat agcaatgata aaatagttac actttctaca
    12721 tgtacctatg aatttgatga tgccagaatg gtgattcatg gtagattgat ttaaaactca
    12781 cagacacagt ttaaaacaat aattttaaac tgtgtcatct tttataccta taatttgtaa
    12841 attagaccac taaaagtatt taatacaaaa taacaaatac atcaaacaat aaaaaacaat
    12901 aaaaaaagac tatgctaaaa acatagtctt tactggagct ggtgatagga atcgaaccta
    12961 caacctgctg attacaagtc agctgctcta ccgttgagcc acaccagcac acctatttta
    13021 ttgtaaatta gcaaaactaa tttttaaaag tta
//
LOCUS       DAMJVK010000104        12747 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.108, whole genome shotgun sequence.
ACCESSION   DAMJVK010000104 DAMJVK010000000
VERSION     DAMJVK010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12747
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.108"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            131..319
                     /locus_tag="PC414_003112"
     CDS             131..319
                     /locus_tag="PC414_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HDF2465489.1"
                     /translation="MAKVCSVCGKGKVSGNQVSHSNKHNKRTWSANLRSVRAIIDGAP
                     KRVKVCTRCLRSGKIERA"
     gene            complement(479..631)
                     /locus_tag="PC414_003113"
     CDS             complement(479..631)
                     /locus_tag="PC414_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate translocator"
                     /protein_id="HDF2465490.1"
                     /translation="MRNNKSKVLLGILCLATGMAVILSMLLPGWIWSALTALILIGCG
                     ALLFFC"
     gene            complement(729..1205)
                     /locus_tag="PC414_003114"
     CDS             complement(729..1205)
                     /locus_tag="PC414_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HDF2465491.1"
                     /translation="MNLKRSNEKREEYRRMYSDKESFLSLIQNFTSVSIAKELTLKNI
                     ELETSFICEYKGKEVDIIYKVFSKSGKVSHYIVLEFQTEMDTEIVPRLKSYREQIWKS
                     FIMKKSLEEIEDKNFKLPKVIPVVLYSGPERLSRKKGILDIVESISEFSNNSSMKN"
     gene            1424..2185
                     /locus_tag="PC414_003115"
     CDS             1424..2185
                     /locus_tag="PC414_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2465492.1"
                     /translation="MEYTVQKLSKLAGISTRTLRYYDEIGLLKPLKINSSGYRIYGQN
                     EVNKLQQILFYRELGINLENIKNIINSPNFDSLSALKEHHNKLLAKRKQIDLLIENVT
                     KTIALKEGKYTMTDIEKFEGFKEKMIDENEKNYGTEIREKYGKDIIDTSNKKLKNMSK
                     QDYENWQNLNIEIISKLKKAFKTGDASSELAQEVAKLHHKWLCYTWNTYSKEAHSSLA
                     QMYVYDERFTSYYDREQPGLAKFLRDAIVIYTRDK"
     gene            complement(2445..4373)
                     /locus_tag="PC414_003116"
     CDS             complement(2445..4373)
                     /locus_tag="PC414_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2465493.1"
                     /translation="MHKRLLTLFKLLNESDDKITCKTLSNHLKVSERTIRNDITSING
                     TLEKNGAIIKIKKGEGYYIDILNLALYQQYLALISDDIMDSSEIPDSPIERNQYILKY
                     ILYNNTYIKLEDLANSLYVSKFTILNDIKRIKPILSKYNLILVSKPYYGVKVEGKEID
                     IRRCISNNMINRNFENYIIGITDREIELFNNVDLIELQRVVLSEINKFNINFLDFNLK
                     NFIIHLAITVSRILDGYCLDNVLDVVLTDFQSNTTVENIFNYIESKYTIIISKADRVY
                     LYNHFITKSSLLDNVSNRVDTKIIEYVEEILEVINNQYTFDLRNDSVLFDDLVLHFKS
                     ILNSKSYNLNKVNPLINTIKSNYPLAFEITLNAIEKVFKNSIYSLTEDEIGYVSLHIG
                     AGIERFFQSNIKCKNVVLVCGSGYGSSRLLEVQLNKVFHDKINILQCLSFNQFLASEL
                     SDVDIIISTIPLNHDSIPIVLVDLKLLKKDIENISKSITNNSHIYSNLLDNFFDKNLF
                     IVNPKIKDKDELIKLMCNKLTQSEIVFPSFAESVFYRESLSSTNIDDFLAIPHPMELS
                     SIRTKICISILNEPIYWSENSTVKLIMMLAINKDDYIKINSIYDILLKIIHDNDIRDS
                     ISNCNDFDNFLSIIKSIV"
     gene            4607..5059
                     /locus_tag="PC414_003117"
     CDS             4607..5059
                     /locus_tag="PC414_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2465494.1"
                     /translation="MVLQEKYIFIDKDLKSKEEVLNFIADSAYILGITTNKEALLKDL
                     LKRENEFPTGIKDGFAIPHTKSVNVKEASILYIKTTNELEWKTLDDSKVRYVFSLLAP
                     HKNEGNIHLKTLSKLATCLMEDDFTSEVKNASDASHLVKYITNKMKEE"
     gene            5062..5370
                     /locus_tag="PC414_003118"
     CDS             5062..5370
                     /locus_tag="PC414_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HDF2465495.1"
                     /translation="MKIVAITSCIAGLAHTPMAAKALEQAASKMGHEIKVEQQGAMGR
                     VNTITKEEAQDADFILIASDQTISGMERFDGKKVIKVKIGIALKKPEAVLTKCIEAIS
                     "
     gene            5484..6590
                     /locus_tag="PC414_003119"
     CDS             5484..6590
                     /locus_tag="PC414_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDF2465496.1"
                     /translation="MSKKNKSLLSELKGHLMTGISYMLPLVIGSSLVVAIPKLTALAV
                     GITSLDAYKNVSGFYHILYLMEQVGWTGIGLLNTVLAGFIAFSIGEKPAMGAGFIGGL
                     IASNTNAGFLGAVIAGFFAGYVCKFLKKKIKISGSASGMMPLIIMPLITVGLTGFLMS
                     VVLAGPLGNINTSLNAWVAEMCQNGTNSVVLALILGAMIGFDLGGPVNKAAWMAGNAL
                     LLEGIYLPAILVNCAIVIPPLGYGLATFIRKSKFSNALAETGKGNIVMGIIGITEGAI
                     PFTLTNPLKLVPVNMIGCALGVGLSALLGVHAIMPPVGGLYGFISIGSGWAYLVGAIF
                     GALVIAILSTTLVDFNEDETTENTEFDEIELDIM"
     gene            6665..9343
                     /gene="mngB"
                     /locus_tag="PC414_003120"
     CDS             6665..9343
                     /gene="mngB"
                     /locus_tag="PC414_003120"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HDF2465497.1"
                     /translation="MKKTKVHVVPHSHWDREWYFTTSRSKIYLMKDFQDILDILEEKE
                     DFKYFTMDAQASLLDDYLKWRPQDKYRIRDLVQRRRLVIGPWYTQTDQLVISGESIIR
                     NLYYGIDRCNEFGEPMRVGYVPDSFGQSAQMPQIYRGFGIDSSLFWRGVSDDMVDTTE
                     FIWKGSDGSKVLAVQIPFGYYYGGNIPEDDSDLKEYLKDIIVKLKVKASTNNVYFPNG
                     FDQAPIRKNLPDILKKANEIDNENEYEISDIENYIDSVRNERNEFTELKGEFLNAKHM
                     RIHKSIFSSRSDLKIMNNKLENYITNVLEPLLTLSYSLGNEYPHLVIKDIWKLMFENA
                     AHDSIGSCNSDTTNEDVYTRYKQARDLSINLLDLHMRLISTQIKNNNEEITLTVFNGF
                     PEVRNEVVEFDTYIPGQDFVIKNKNGKILNYIIKEKEDITNYILTQTIRLNPSKKIYI
                     PKTVYRAKIALSVKDIPPMGYTQLVFELDTKNKCEIETSNCKEIENKYYKITANKNGS
                     LEILDKESNKTYKNQAVIEDNGDDGDSYNYSPPRKDIYVSSLDSVSSVEVLKSNIQEE
                     MILKYSLTIPASLEERAIGKVSVKMPVTMKITLEPKEQIIKFNVNIKNNQALSHRVRV
                     LFNTEIASKFSIADQQFGIIQRPTVLEKDLALWKRDNYSWQEKPITIEPMQSFVTLED
                     GQRGIALITGCVREYQIVGDNLDTIALTLFRSFGYMGRENLLYRPGRASGEKIIATPD
                     AQLLKELNFDFGLYIYNNKFDEANVANTAKRFLTPIQIYEYADFLNGRLIFAFNDEKQ
                     IYENEFSLMNVNNSNFTVSAIKKEEKGDGIVVRIFNGMKNEDAKGSLNINKNVNDAYS
                     AMLDEIYDNTSKLKVNTKTVEVNSLSHCKVQTIVIK"
     gene            complement(9660..11993)
                     /locus_tag="PC414_003121"
     CDS             complement(9660..11993)
                     /locus_tag="PC414_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2465498.1"
                     /translation="MDTKLKKFKDKYIVTIIVFILMLSASLGMISQYSTIQASAKGGA
                     ENPFEQESFVDSIYKGSYVLYHNMKEEQEGKMIQPSKLFINEETMNYIKNVSKEKDTY
                     DGSETYYTDDSINADFNENFENWEHFIKASGKNLKYYLVDKESNFEFFNDNKELGVLD
                     TTKNDASKENSDENQSKNTEMNDEERQKQITDIKENYRFYVVMNFDKDGKVNIVDSYG
                     VNQKIINQMLLSKAREDLMDTSLGGNSISYKLSPIKNITLVYAVPKVISKGDSVEYGY
                     GNTYFDDGIISYISNVENNSYYKISGMILKIILAIVVIVALAFPYRKSKELLGFDTLS
                     RIPLEILFIALAIIQGLVDLYPTVIISNTLSGNYVEYLINNDISAKMASILVSLMNLT
                     YWFVIFSIIFVFIILLKHILNVGIREYFTKHTVTGMFFVWLKKAGKTVINQIRTINLK
                     EKPNKTIVTILGINLLIIVIMCSMWFFGIILALIYSVVLFKILSDYSKKTIYEYNQLL
                     DVTKKISDGNLDVNMEKDLGFFNPIKDELGNIQTGFKKAVDEEVKSQKMKTELISNVS
                     HDLKTPLTSIITYIDLLKDESITDENRKLYIDTLDRKSQRLQHLIEDLFEVSKANSGD
                     VYLNIVNVDIVSLMKQTLLELDDKLADSSLIVKNNFSNEKIILPLDSQRTFRVFENLI
                     INISKYAMPNSRVYIDILETDNKVNIMLKNMSASEIDFSVDDIMERFVRGDKSRNTEG
                     SGLGLAIAKSFIELQGGKMNISVDGDLFKVTITFNKN"
     gene            complement(11968..12684)
                     /locus_tag="PC414_003122"
     CDS             complement(11968..12684)
                     /locus_tag="PC414_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2465499.1"
                     /translation="MNSYNILVVEDEKEIADAIEIYLLNQGYNVFKGYNGLEGLKVIE
                     NQEIHLAIVDIMMPKMDGITLTMKLRENYNFPVIMLSAKSEEVDKIMGLNIGADDYVT
                     KPFKPLELLARVNSQLRRYTKYLNMVGNKEQKVESDDSVFAIGGLELNENTKEVSIDG
                     KYIKTTPIEFKILSLLMRNAGRVFSADEIYERVWNENAVNTDTVMVHVRNIREKIEID
                     PKNPKYLKVVWGVGYKIEKI"
BASE COUNT         4762 a         1779 c         1664 g         4542 t
ORIGIN      
        1 tatgataata agaaattctt caatatgtgt aaggcaaata tatatttcta ttgcaaattt
       61 ttgttattta tgataatata aatatgtttt tagtctttaa atagttatca ttttaaggag
      121 gtgtgcgtag atggcaaaag tatgtagcgt atgtggtaaa ggtaaggttt ctggaaacca
      181 agtatcacac tcaaataaac ataataaaag aacatggtca gctaacttaa gaagcgtaag
      241 agcgattata gatggagctc ctaagagagt aaaagtttgt actagatgtt tacgttctgg
      301 taaaattgaa agagcttagt aaccacgttt aaaagcctac ttccgtaagt aggcttttta
      361 atttaatcat ttttactctt tctcataaat tttaggatta tacttgaaat ccattttggt
      421 aattttattg ttactacttt catcaatact caccccacac tttatagtta aaactatttt
      481 aacaaaaaaa taataatgct ccacatccaa taagtattaa tgcggtaagt gctgaccaaa
      541 tccagccagg aagcaacatt gacagaatta cagccattcc tgttgctaga caaagtattc
      601 ctagcaatac cttagacttg ttatttctca tggaacacct ccacatagaa cctgtatcta
      661 taaatatttt catattcaag tttatgcgac agttttccat tttagaccaa taaacacatt
      721 atgtaatttt aatttttcat actagaatta ttactgaact ccgatataga ttctacaata
      781 tccaaaattc ctttttttct acttaacctt tcaggtccac tatataaaac aactggtata
      841 actttaggaa gtttaaaatt tttatcttct atttcttcaa ggcttttttt cataataaaa
      901 cttttccaaa tttgttctct atacgatttc aatcttggga caatttcagt atccatttct
      961 gtttgaaatt ctaacactat ataatgtgat actttgccgc ttttagaaaa taccttataa
     1021 attatatcaa cttcttttcc tttatattca catataaaag atgtttctaa ctctatattt
     1081 ttaagagtta attcttttgc tattgagaca cttgtaaaat tttgaatcaa acttaaaaaa
     1141 ctttctttat ctgaatacat tcttctgtac tcttctcgtt tttcattgct tctttttaaa
     1201 ttcatacagt tcctcctaga ctatagacct tctatttttt agtaaagata gtctataaat
     1261 tttatttttt aattttcact agtgagtatt gacagttgtg tgtaaaatat ccagtaaaga
     1321 tattttttta tatttttatt aaaagatata aatttttata ttttaatgta ttgacattta
     1381 cgttgcgtaa aagtgtatat ttaatttata ggaggtacta cttatggaat atacagttca
     1441 aaaattaagt aaactagctg gtataagtac tagaacactt agatattatg atgaaatagg
     1501 tcttcttaag ccacttaaaa taaattcttc aggttatcgt atatatggtc aaaatgaagt
     1561 taataaactt caacaaatac ttttttatag agaattagga attaacttag aaaatataaa
     1621 aaacattata aattcaccaa acttcgatag tttaagtgcg ttaaaagaac accataataa
     1681 attactagcc aaaagaaagc aaattgattt actaatagaa aatgtaacta aaactatagc
     1741 tctgaaggag ggaaaataca ctatgacaga tatagaaaaa tttgaaggtt ttaaagaaaa
     1801 gatgattgat gaaaatgaaa aaaattatgg aactgaaatt agagagaaat atggtaaaga
     1861 tattatagat acatcaaata aaaaacttaa aaatatgtct aagcaagatt atgaaaattg
     1921 gcaaaaccta aatatcgaaa taattagtaa acttaaaaaa gcttttaaaa ctggtgatgc
     1981 ctctagtgaa ctggctcagg aagttgcaaa attacatcat aaatggttgt gttacacttg
     2041 gaacacatac tctaaagaag ctcattcctc tctagcacag atgtatgtat atgatgaacg
     2101 ttttacatca tattatgata gagaacaacc tggattagct aagtttttaa gggatgctat
     2161 agtaatatat acaagagata aataatataa aataataaag gtggtgtctc aaaatgaact
     2221 ttttagttca tgagacactc ttttttgtat gaaatcagat atatttttat cattttatca
     2281 atgaaaaaca ggtttctctc tctattttga gacagaatct ttattgtata ataattataa
     2341 tcaatacaaa ataataacaa attatacaaa acttacttat actatttatg ctaaaaacaa
     2401 aaaatatttt attaaagttt ttaaaactta tgtaatataa aaatctatac tatagacttg
     2461 attatggaaa gaaaattatc aaaatcatta caattagata tactatctct aatatcattg
     2521 tcatgaatta tctttaaaag gatatcataa atggagttta tttttatata atcatcttta
     2581 tttatagcca acatcatgat taatttaaca gtagaatttt cactccaata tattggttca
     2641 ttcaatatgg aaatacaaat cttagttctt atagagctta attccatagg atgaggtata
     2701 gctaaaaaat catctatatt ggtagaagat aaactttctc tgtaaaatac agattcagca
     2761 aatgatggaa atacaatttc actttgagtt aatttgttgc acatcaactt tataagctca
     2821 tctttatctt tgattttagg attaacaata aataaatttt tatcaaagaa attgtccaaa
     2881 agattactat aaatatggga attattagtg attgacttag atatattctc tatatctttt
     2941 tttaatagtt ttaaatccac taatacaata ggaatactat catgatttaa aggaattgtc
     3001 gatattatta tatctacatc tgacaattca ctagctaaaa attgattaaa tgatagacat
     3061 tgaagtatat ttattttatc atgaaaaact ttgtttaatt gaacctctaa taacctactt
     3121 gaaccatacc ctgaaccaca tactaatact acgtttttac acttaatatt actttgaaaa
     3181 aatctctcta ttccagcacc tatatgcagg gatacatatc caatttcatc ttctgttaaa
     3241 gagtaaatac tatttttaaa aactttttct atagcattta aagttatttc aaatgctaaa
     3301 ggataattac tctttattgt atttataagg ggattcacct tatttaaatt ataagattta
     3361 gaattgagaa ttgatttaaa gtgcagtact aaatcatcaa ataatacact atcatttcta
     3421 aggtcaaaag tatattgatt gttgattact tctaaaattt cttcaacata ctctataatt
     3481 ttagtatcta ccctattgga aacgttatcc aaaagagaac ttttagttat aaaatgatta
     3541 tatagataaa ctctgtctgc ctttgaaata attatagtat atttactctc aatataatta
     3601 aatatatttt caacagttgt attcgattga aaatctgtta aaacaacatc taaaacatta
     3661 tctaagcagt aaccatcaag tattctactt acagtaatag ctaaatggat aataaagttt
     3721 tttagattaa aatctaggaa attgatatta aatttattaa tttcacttaa cactaccctt
     3781 tgaagctcta ttaaatctac attattaaat aattctattt ctctatctgt aattccaatt
     3841 atatagtttt caaaattcct atttatcata ttattagaaa tacatcttcg aatatctatt
     3901 tccttacctt caactttaac tccataatat ggcttagaaa ctaatattaa attatacttt
     3961 gacaaaatag gctttattct ttttatgtca tttagtattg taaatttact cacatacaag
     4021 ctattagcta aatcttctag ttttatatat gtattgttat agagaatata ttttaaaata
     4081 tattgatttc tttctatagg agaatctggt atttcacttg aatccataat atcatcagat
     4141 attaatgcta gatactgttg gtataatgct aaattaagaa tatctatata atatccttct
     4201 ccttttttta ttttaataat agcaccattt ttctctaaag ttccatttat agaagtaata
     4261 tcatttctta tagttctctc actcactttt aaatgattac ttaaagtttt acaagtaatt
     4321 ttatcatcac tttcatttaa taatttaaac aacgtgagca atctcttgtg cataaatatc
     4381 cccccttata gaatttcttc taaattatac tatttacact catataatac aattactcta
     4441 ataaaattgt attataataa tttatttcca cttctatatg gaaattagtt attatcataa
     4501 tataaatttc catgtcgaag tggaaattag ttatatttaa tttaattagg ataatgatta
     4561 caatataaat ataagttaag acattatgaa tggagtggaa aaatatatgg tactacaaga
     4621 aaaatatata tttatagata aagacttaaa aagcaaagaa gaagtattaa attttattgc
     4681 agatagtgct tatatactag gtattacaac taataaagag gctcttttaa aagaccttct
     4741 aaaaagagaa aatgaatttc caacaggaat aaaagatgga tttgcgatac ctcatacaaa
     4801 gagtgtaaat gtaaaggaag cttccatact ctatattaaa acaactaacg agttagaatg
     4861 gaaaacttta gatgatagta aggttagata tgtatttagt ttgttagccc cacataaaaa
     4921 tgaaggaaat atacatttga aaactctatc taaattagcc acatgtttaa tggaggatga
     4981 ttttacaagt gaagttaaaa atgctagtga tgcaagtcat ttagtaaaat atataactaa
     5041 taaaatgaag gaggaataat tatgaaaata gtagctataa catcatgtat agcaggatta
     5101 gcacacacac ctatggcagc aaaagcttta gaacaagctg catcaaaaat gggtcatgaa
     5161 atcaaagtag aacaacaagg tgctatggga cgagtaaata ctataacaaa agaagaagca
     5221 caagatgcgg actttatact tatagcatct gaccaaacta tatctggaat ggaaagattt
     5281 gatggtaaaa aagtgataaa agtcaaaata ggtatagcgt taaaaaaacc agaagctgtc
     5341 ttaacaaagt gtatagaagc tatttcttaa taaaaaaatc tcactctaat atagaatgag
     5401 aaaaatcaaa aattaaaaaa ttgtctaatg taattataac atattatcaa aaaatataac
     5461 aaacttatat aggaggatta aaaatgagta aaaaaaataa atctctttta agtgaactta
     5521 agggacactt aatgacaggt atctcatata tgttgccact tgtaataggc tcatctttag
     5581 ttgtagctat cccaaagctt acagctttag cagtgggtat aacaagttta gatgcttata
     5641 aaaatgtatc tggtttttat catattcttt acttaatgga acaagttggt tggactggca
     5701 taggtctatt aaatacggta ttagcaggat ttatagcatt ttcaataggt gagaaaccag
     5761 ctatgggtgc aggttttatt ggtgggctca tagcaagtaa tactaatgca ggattcttag
     5821 gagcagtaat agcaggtttc tttgctgggt atgtttgtaa gtttttaaag aaaaaaatta
     5881 aaatatctgg gtcagcatca ggaatgatgc cacttataat aatgccactt ataacagttg
     5941 gattaactgg ttttttaatg agtgttgtat tagcaggacc tttaggaaac ataaatactt
     6001 cattaaatgc ttgggtagct gaaatgtgtc aaaatggaac aaattcggtg gtattggctt
     6061 taattttagg cgcaatgata ggattcgatt taggtggacc agtaaataaa gcagcttgga
     6121 tggcaggaaa tgcattattg ttagagggaa tctatctacc agcgatattg gtaaactgcg
     6181 caatagttat acctccacta ggatatggtt tagctacatt cataagaaaa tcaaaatttt
     6241 ccaatgcatt agctgaaaca ggaaaaggca atatagttat gggtataatt ggaataacag
     6301 aaggtgcaat tcctttcact ttaacaaatc cattaaaatt agttcctgtt aatatgatag
     6361 gatgcgcttt gggtgtagga ttatcagctt tgttaggtgt acatgctata atgcctccag
     6421 ttggtggatt atatggattt atatcaatag gttctggatg ggcttactta gtaggtgcaa
     6481 tatttggagc tttagtaata gcaatattat caacaacttt agtagacttc aatgaagatg
     6541 aaactacgga aaatacagaa tttgatgaaa tagagttaga tataatgtaa attaaaaact
     6601 taattttaga taattgagaa ttgagtgtac tctcaattct caattaaatt ttgggaggat
     6661 ttttatgaaa aagacaaaag tacatgtagt tccacacagt cattgggata gagaatggta
     6721 ttttacaaca tcaaggtcaa aaatatattt aatgaaagat tttcaagata tattagacat
     6781 attggaagaa aaagaagact ttaaatactt tactatggat gcacaagctt ctctcttaga
     6841 tgattattta aaatggagac ctcaggataa atatagaata agagatttag ttcaaagaag
     6901 aagattagtt attggacctt ggtatactca aacagaccaa ttggttatat caggagaatc
     6961 tataattaga aacctttact atggaataga tagatgcaat gaatttggtg aacctatgag
     7021 ggttggatat gtaccagatt cttttgggca atcagctcaa atgccacaaa tatacagagg
     7081 ttttggaata gatagttcat tattttggcg tggagtttct gatgatatgg tagatacaac
     7141 agagtttata tggaaaggtt ctgatggttc aaaagtatta gcagttcaaa taccatttgg
     7201 ttattactat ggaggtaata taccagaaga tgattcagat ttaaaagaat atttaaaaga
     7261 tataatcgta aaacttaagg ttaaagcttc tactaataac gtatattttc caaatggatt
     7321 tgaccaagca ccaataagaa aaaaccttcc tgatatatta aaaaaagcta atgaaataga
     7381 taatgaaaat gaatacgaaa taagtgatat agaaaactat atagattcag taagaaatga
     7441 aagaaatgaa tttacagagc ttaaaggtga atttttaaat gctaaacata tgagaataca
     7501 caaatccata ttctcctcaa ggtcagacct taaaataatg aataacaaat tagaaaatta
     7561 tattacaaat gtacttgaac cgttattaac attaagttat tcacttggaa atgaataccc
     7621 tcacctagtt ataaaagata tatggaaact tatgtttgaa aatgcagctc atgacagcat
     7681 aggttcttgt aatagtgaca caacaaatga ggatgtatac acaagataca aacaagccag
     7741 agatttgagt ataaatttat tagatttgca tatgagactt ataagtactc aaatcaaaaa
     7801 taataatgaa gaaataacat taacagtatt caatggattc ccagaagtca gaaatgaagt
     7861 tgtagaattt gatacttata ttccaggtca agattttgtg ataaagaata aaaatggaaa
     7921 aattcttaat tatataataa aagaaaaaga agatattaca aattatatat taactcaaac
     7981 aataagatta aacccaagta aaaaaatata tatacccaaa actgtatata gagcaaaaat
     8041 tgcactttca gtaaaagata taccaccaat gggatatact caactagttt ttgaattgga
     8101 tactaaaaat aaatgtgaaa tagaaacaag taattgcaag gaaatagaaa acaaatacta
     8161 caaaataaca gccaataaaa atggttcatt agaaatatta gataaagaat ctaataagac
     8221 ttataaaaac caagctgtaa ttgaagacaa tggagatgat ggagattcat acaactattc
     8281 tcctccaaga aaagatattt atgtttcatc tttagattct gtatcttcag tagaagtgct
     8341 aaaatcaaac attcaagaag aaatgatact taaatattca ttgaccatac cagcttcact
     8401 ggaagaaagg gctataggta aagtttctgt gaaaatgcca gtaacaatga aaatcacctt
     8461 agaacctaaa gaacaaataa ttaagtttaa tgttaatata aaaaataacc aagcactatc
     8521 tcatagagta agagttttat ttaatacaga aatagcatct aagttttcta tagcagatca
     8581 acaatttgga ataatacaaa gaccaacagt attagaaaaa gaccttgcat tgtggaaaag
     8641 agacaattac tcatggcaag aaaaacctat aactatcgaa ccaatgcaaa gttttgttac
     8701 tttagaggat ggacaaagag gaatagcatt aataactggt tgtgtaagag aatatcaaat
     8761 agttggtgat aatttagata caatagcatt aacactattc aggtcatttg gatatatggg
     8821 aagagaaaat ttactttata gaccaggaag agcttctgga gaaaaaataa tagcaacacc
     8881 agatgcacaa ttacttaaag agttaaactt tgactttggt ctttacatat ataacaataa
     8941 atttgatgaa gcaaatgtag ctaatacagc taagagattc ttaacaccta tacaaatata
     9001 tgaatatgct gattttttaa atggtagact tatatttgca ttcaatgacg aaaaacaaat
     9061 atatgaaaat gaatttagtt tgatgaatgt aaataattca aatttcacag taagtgcaat
     9121 aaagaaagaa gaaaaaggag atggaattgt agttagaatc ttcaatggta tgaaaaatga
     9181 ggatgctaag ggaagtctta acatcaataa aaatgtcaat gatgcttata gtgcaatgct
     9241 cgacgaaatc tatgataata ctagcaagct taaagtaaat actaaaactg tagaggtaaa
     9301 ttctttatca cattgtaaag tacaaacaat agtaattaaa taatataaaa tagcttatac
     9361 aagatttcaa aataaattgc tagtaccatg ttaatagtaa agccagatat tatgctattt
     9421 ataagctctg taacactaaa taacattttg taaaataaaa aggtgaacct tgtatattag
     9481 gattcacctt ttttaaataa atcaacattc tgttttaaaa taacatttat aagctgtttt
     9541 gaaataatgc ttacaaatta ttttaaaaca atacctataa atttttattt aaagaataca
     9601 cataaatatt tccagactct tttatcctat agactttata attattaaag aagtctaatc
     9661 taatttttgt taaaagtaat tgtaacttta aataaatcac catccacact tatattcatc
     9721 ttaccaccct gtaattctat aaaacttttt gctatcgcta aaccaagacc agaaccttct
     9781 gtgttacgtg atttatctcc tcttacaaat ctctccatta tatcatctac actaaaatca
     9841 atctcagatg cagacatatt tttaagcata atatttactt tattatctgt ttctaaaata
     9901 tcaatataca ctcttgaatt tggcattgca tatttgctta tatttattat aagattttca
     9961 aatactctaa atgtacgctg actatctaat ggtaatatta ttttttcatt agaaaagtta
    10021 ttctttacta ttagagatga atctgcaagt ttgtcatcta attctaaaag tgtctgcttc
    10081 attagagata caatatccac atttactata ttcaaataaa catctccact atttgccttg
    10141 cttacttcaa acaaatcttc tattagatgt tgcaatcttt gtgactttct gtctaatgta
    10201 tcaatataca acttacgatt ttcatctgtt atactttcat cttttaataa atctatataa
    10261 gtgattatag atgttagagg tgtttttaag tcatgtgata catttgatat aagctctgtt
    10321 ttcatctttt gactcttaac ctcttcgtca acagcttttt taaatccagt ttgaatattt
    10381 cctaattcgt cttttatagg attaaaaaat cctaaatctt tttccatatt tacatctaag
    10441 tttccatctg aaattttctt tgtaacatct aaaagttgat tatattcata tattgtcttt
    10501 ttagagtaat ctgacagtat tttaaataat actactgaat atattaatgc gagtattatt
    10561 ccaaagaacc acatactaca cattataact ataatcaata aatttattcc caaaattgta
    10621 actatagttt tatttggctt ttcttttaga tttattgttc taatttgatt aataactgtt
    10681 tttcctgctt tttttagcca tacaaaaaac attcctgtca cagtatgttt tgtaaaatac
    10741 tctcttattc caacatttaa aatatgtttt aaaagtatta taaatacaaa tattattgag
    10801 aatattacaa accaataagt taaattcatt aaacttacca atatactagc cattttagca
    10861 cttatgtcat tgtttataag gtactctaca taattcccac tcaaggtatt acttattata
    10921 acagtaggat ataaatctac aagcccctgt ataatagcaa gagcaataaa gagtatctct
    10981 aacggtattc ttgataatgt atcaaatcct agcaactctt tagattttct atatgggaat
    11041 gctaaagcta caataacaac tatagctaat ataattttta aaatcattcc agaaatctta
    11101 taataagaat tattttctac attgcttata tatgaaatta taccatcatc aaaataagta
    11161 tttccataac catactctac actatcacct ttacttatta ctttaggtac agcatatact
    11221 aatgttatat tttttattgg agataactta tatgaaattg aatttcctcc taaacttgta
    11281 tccattaaat cttctcttgc tttagataac aacatttgat taataatttt ttgatttact
    11341 ccataagaat ccacaatatt tacttttcca tctttatcaa aattcattac aacgtagaat
    11401 ctatagtttt cttttatatc tgtaatctgc ttttgtcttt cctcatcatt catttctgta
    11461 ttttttgact gattttcatc agaattttct tttgaagcat cattttttgt tgtatcaagt
    11521 actcctaatt ctttattatc attaaaaaat tcaaaattgc tttctttatc aactaaatag
    11581 tactttaaat tctttcctga cgctttaatg aaatgctccc aattttcaaa gttttcatta
    11641 aaatctgcat taatactatc atctgtatag taagtttctg aaccatcata agtatctttt
    11701 tctttactta cattttttat atagttcata gtttcttcat tgataaatag ttttgatggt
    11761 tgtatcatct taccttcttg ttcttccttc atattatgat ataaaacata acttccttta
    11821 tatatactat ctacaaagct ttcctgttca aatggattct ctgctccacc tttagcactt
    11881 gcttgtatag tagaatattg actaatcata cctaatgatg ctgataacat aagtataaaa
    11941 actataatag taactatata tttatcttta aattttttca attttgtatc caacacccca
    12001 caccaccttt aaatatttag gattttttgg gtcaatttct atcttttccc ttatattcct
    12061 cacatgtacc ataactgtat ctgtatttac agcattctca ttccaaactc tttcatatat
    12121 ttcatcagca gaaaaaactc ttcctgcatt tctcataagt aaacttagta ttttaaattc
    12181 tataggagtt gtttttatat actttccatc aatactaact tcttttgtat tttcatttaa
    12241 ttctaatccg cctattgcaa acacactatc gtcactttct accttttgct ctttatttcc
    12301 aaccatgttt agatacttag tgtaccttct aagttgtgaa ttaactctag ccaatagttc
    12361 taatggctta aaaggttttg ttacataatc gtctgctcct atattaagcc ccattatttt
    12421 gtcaacttcc tctgattttg cagatagcat tattactgga aaattatagt tttctctaag
    12481 cttcattgta agtgttatac catccatttt aggcatcatt atatcaacta tagctaaatg
    12541 tatttcttgg ttttctataa ccttaagacc ttctaatcca ttgtatccct taaaaacatt
    12601 atatccttga tttaatagat atatttctat agcatctgct atctcctttt catcttctac
    12661 caccaaaata ttatatgaat tcattctaat ctcctttata ttaatttata aattttcttt
    12721 acatatatga ttatacaata tgataga
//
LOCUS       DAMJVK010000105        12420 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.109, whole genome shotgun sequence.
ACCESSION   DAMJVK010000105 DAMJVK010000000
VERSION     DAMJVK010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12420
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.109"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            <1..321
                     /locus_tag="PC414_003123"
     CDS             <1..321
                     /locus_tag="PC414_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDF2465500.1"
                     /translation="VEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYKGLKEAGIDS
                     EITQYSFCTNGSHYAGEAGIRTIGFGPSKENLAHTIDEYIELDQLFKGAEGYYGILKS
                     VYKK"
     gene            337..1920
                     /locus_tag="PC414_003124"
     CDS             337..1920
                     /locus_tag="PC414_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HDF2465501.1"
                     /translation="MRILIKHGLIVDGNKTPAYEGDILIENEKILKISQDLNEEADKV
                     IDAKGRVICPGFIDTHSHSDLVILVNPYNEVKIRQGITTEVLGQDGISMAPLPQEHIS
                     SWRKNLAGLDGESDEIDWKYETTENYLKMMDYNGVGLNETYLVPHGNVRMEAMGLEDR
                     PATKEEIQKMCEITERELKAGAIGLSTGLIYIPCAYSLTEEIIEMCKVVAKYDGVFVV
                     HQRSEADTILTSMEEIIEIGKQSGVKVHFSHFKVCGKANWKYIPQVIELLEKAEKEGI
                     RVSFDQYPYAAGSTMLGVVLPPWAHSGGTDKLIERLSDENERAKMKKDIANGIEGWDN
                     FIEFAGIDQIFVTSVKTEKNKDTIGKNLLEIGKMRGKDPLDATFDLLKEEENAVGMVD
                     FYGLEEHIIGFMKRDEQNVCTDGLLAGKPHPRAYGSFPKILGRYVRELNVLTIEEAVY
                     KMTKKAATSFSIKDRGELKEGYFADIVIFDKDTVSGCDDFINSMQYPTGIDYVIINGN
                     CVVEEGKYNKIKAGKVLKN"
     gene            1948..3276
                     /gene="ssnA"
                     /locus_tag="PC414_003125"
     CDS             1948..3276
                     /gene="ssnA"
                     /locus_tag="PC414_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HDF2465502.1"
                     /translation="MILVGNGRLISQDSLNPYMEDGCVVINDNLIEDIGTTEEMKAKY
                     PVHEFIDAGKKIIMPGLINSHMHIYSSFARGMAVPGKPSENFLEILNNLWWRLDKQLT
                     LEDTKYSAYATYIECIKNGVTTVYDHHASPNAIEGSLFTISDVAKDLGIRTCLCYEVS
                     DRDGGNTIQQGINENVNFIKYAQKDDTDMIKGMFGLHAAFTLSDDTLNKCASEMAGLD
                     TGYHVHVAEGLADLQYNVDKYGKRVLERFNDFGVLKEKTIAVHCIYISAEEISLLNKT
                     NTFVVHNPESNMGNAVGCSPAIELLKRGVTVGLGTDGYTSDMFESLKVANIIHKHHLK
                     DPRVGFEESQTMLFDNNRKIAKAYYKNDLGVLKKGAYADVIVVDYIPHTPLTENTIGG
                     HTIFGMSGRSVDTTIINGKVLMKDRKLINIDEESILAKSRELSQNLWNRI"
     gene            3475..4827
                     /locus_tag="PC414_003126"
     CDS             3475..4827
                     /locus_tag="PC414_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HDF2465503.1"
                     /translation="MSRDKSPKIAPVDERLPVSKSWVFSLQHVLAMCAGAVAVPMMIG
                     SAARLDHASIVFLINACLFMAGIGTLIQSVGIGNIVGAKIPVIEGTSFAAVAGMTAIA
                     SSYSDSQVAIQTVFGSVIVAGLFCILLAPVFEKLIRFFPSVVTGTVVMVIGISLFPVG
                     IKWITDNKLEAVEPNSLALALAVLAITLLSFKFLKGIWNSTAILVGIVLGTLLSMAVG
                     MADFSAVNGVGWLNLNVPLKFGMPRFEPSAILSLCLIMLVLMTESVGNMIAIHEMVDK
                     EVTGKNIKRGLFGDGISTALSGIFNSFPITPFGQNTGIVGLTGIKSRFIAVYAGVILL
                     VLGFLPKFAAIIGAIPKPVLGGVGFAMFGMVMVGGVKTLSKVKFDGTKNGVIVAVSVG
                     LSMIPLTNPDFYHNFPTWVQTIFHSGITTGSLSAVLLNIFFNELGSKKEKNNKVEEAK
                     "
     gene            5019..6128
                     /locus_tag="PC414_003127"
     CDS             5019..6128
                     /locus_tag="PC414_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2465504.1"
                     /translation="MANLKVNLYGMEFLNPIMTAAGPGAKDGALCQEAAKGGAGAICT
                     KTISVLPADVPRPCMAKTNSGFLNTELWSELPKEQWIEEEYKKVKETGLPVIVSMGYS
                     AEQIAEVAPLVKPYADAVELSTHYVGTDVAPIVNALKAAKAVLDVPVFMKMSPHTDIQ
                     TIAKAVEEAGADGLVMINSFGPCMAIDVNTGYPIMGSKAGYGWLSGAPIRPLAIRCIY
                     EASQVVNIPIIGVGGITCGKDVAEMMMAGASAVQVCTEAILKGPSIYGKIAKELNEFL
                     DENGYADVNEIKGLTHKKMAARGFRTHSVAPVVDDNKCIKCKQCVTSCVYEALEVTDK
                     LNIDADKCFGCGLCVTRCPKGALSICYNQVEAMNE"
     gene            6411..7802
                     /gene="hydA"
                     /locus_tag="PC414_003128"
     CDS             6411..7802
                     /gene="hydA"
                     /locus_tag="PC414_003128"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HDF2465505.1"
                     /translation="MGIVLKGGKVVTSEKTYCADVRIEDEKIVDIGLDISKENDEIVD
                     VSDSYVIPGGIDTHTHFDLDTGVTKTADNFETGTKAAIVGGTTTILDFATQAKGCSLK
                     DGLEEWHEKAKDKSYSDYGFHMAITDWNDSVCNEMEDMVKEGVSSFKLYMAYKGSLQV
                     DDGVIFEALRKAEEIGGIIGFHCENGDIICELVDKAKSENNLSPKYHQLTRPAIMEAE
                     ATSRLMKIAEVANSKVYVVHLSCKESLEEVLKAKQRGVDVIVETCPQYLLLNDELYDL
                     DGFESAKYVMSPPLRNKENNEILWNAILDNQIDTIGTDHCSFNYKGQKELGINDFSKI
                     PNGSPGVEHRMSLLYTYGVCKNKISINKMVEVTSTNAAKLFGMYPNKGTIEIGSDADI
                     LVLNPNKKSKIEAKKQLQNVDYTPYEGYEVDCQIEHVYLRGKEVVRDGELICNTPSGR
                     YINRRLTKGAREW"
     gene            7796..10357
                     /gene="xdh"
                     /locus_tag="PC414_003129"
     CDS             7796..10357
                     /gene="xdh"
                     /locus_tag="PC414_003129"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent xanthine dehydrogenase"
                     /protein_id="HDF2465506.1"
                     /translation="MVEFILNGEKVVAKEGKKLLDFLREDMNITSVKNGCSEGACGTC
                     MVIIDGKATKACVFKTDKLAGKNITTIEGLSERERDVFAYSFASQGAVQCGFCIPGMV
                     ISSKALIDVNNNPTEDDIKKALKGNICRCTGYVKIIKAVDLAAKIFRENIDVPKVNCK
                     GLVGENLPRVDAVGKALGYSEYVDDMRIEGMIYGSAVRAKYPRALVKKIDIEEAKAYP
                     GVVAVLTAKDIPGTIKVGHLKKDWDTLIPEGEITRYLGDGIVLVAAETREALEEAKKL
                     VKIDYEELTPLSTTADALREDAPKIHETGNILVKEHLVRGNAKEKIENSKYVVTKRYT
                     TPATEHAFLEPECAVAGPYEDDGVIIYSTDQGVFATQHECADMLGLPYEKVRVINKMV
                     GGGFGGKEDMSVQHHAALLAYHTKRFVKVQLTRKESIIVHPKRHATEMEFTTACDENG
                     YLTGMKATIISDTGAYASLGGPVLQRLCTHAAGPYNYQDIDITGTAVYTNNPPGGAFR
                     GFGVTQSCFATECNLNLLAEMVGISPWEIRYKNAIRPGQVLPNGQFADEGTAMAETLE
                     AVKEEYEKHPRAGIACAMKNSGVGVGIPDVGRCRLVIKDGKVHIRTSASCIGQGLGTV
                     MTQVVCETANLLPENIVHEVPDTHLTPDSGNTTASRQTVFTGEATKVASNKLKEALDS
                     GKTLLDLEGEEFYGEYESITDKMGCDKENPVSHVAYGYATQVVILDENGKLEKVVAAH
                     DVGRAINPISLEGQIEGGVVMGLGYALTEDYPVVNSIPTAKFGTLGLFKSTDIPEIQA
                     LIVEKNKDLLAYGAKGVGEICTVPTAPAVQNAYYVFDKEFRTELPLQNTPYSKKK"
     gene            10509..11855
                     /gene="rpoN"
                     /locus_tag="PC414_003130"
     CDS             10509..11855
                     /gene="rpoN"
                     /locus_tag="PC414_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HDF2465507.1"
                     /translation="MNFNNSLELTQSQKLIMTTQLKQSLSILNMSKLELEEEIKREAE
                     NNPLVEVEKSGEINWEEYIKDMDRSRRLDRTEISYNPDNEVNLENLVKYSSNLYEDLK
                     FQISLYKLTDKELEVCEYIIDSLDEDGYLRTEEKVIVDTLKIDEELFEKCLISVQQLE
                     PSGVGARSLSECLMIQMASLGIYNEILEEIVTKDLNLIANNKYKEISKKYNMSLQKCV
                     ELINIIKTLDPKPGRTCSVEKSVYIQPDVTVEKIDDEFIVYLNEKDSYQIRINSYYKD
                     ILKNSQSDESAKEFIKERLNSATGLMKNIESRKTTVLKIAEEIVKAQDEFLRKGTKYI
                     KPLKMKDIAEKLEFHESTISRGVNGKYMLTPFGVYEFRYFFSSAIETENNEMASSTSI
                     KKIIKETIKDENKKKPLSDDHISKLLKEKGINVARRTVAKYREELGIPSSSKRKEY"
BASE COUNT         4970 a         1428 c         2512 g         3510 t
ORIGIN      
        1 gttgaaaaag ctgattgcta tacaggaaat actatagaaa gtgaaagatt cttcccaggt
       61 tggctatatg atgaagaaga tgaatttgtt caagcagcgt ataagggatt aaaagaagct
      121 ggaatagatt cagaaataac tcaatactca ttctgtacga atggaagtca ctacgcaggt
      181 gaggctggaa taagaactat aggatttggt ccttctaagg aaaatcttgc tcatacaata
      241 gatgagtata tagaattaga ccaattattc aaaggtgcag aaggatatta tggtatatta
      301 aaatcagttt ataaaaaata attaggagat ggttttgtga gaattttaat aaaacatggg
      361 cttatagtag atggaaataa aacaccagca tatgaaggag atattttaat agaaaacgag
      421 aaaatattaa aaatatcaca agatttaaac gaagaagctg acaaagttat agatgcaaaa
      481 ggtagagtta tatgtccagg tttcatagat actcatagcc attcagacct agttatttta
      541 gttaatccat ataatgaggt aaagattaga cagggaatta caacagaagt gttgggccaa
      601 gatggtatat ctatggcccc tctacctcaa gagcatatat cttcttggag aaaaaattta
      661 gcaggtctag atggtgaaag tgatgaaatt gattggaaat atgaaacaac agagaattat
      721 ttaaaaatga tggattacaa tggggtaggt ttaaatgaaa cttatttagt acctcatgga
      781 aacgttagaa tggaagctat gggacttgaa gatagacctg ctactaaaga agaaattcaa
      841 aaaatgtgtg aaatcacaga gagagaatta aaagcaggag ctataggatt gtctactgga
      901 cttatatata taccttgtgc atactcattg actgaagaaa ttatagagat gtgtaaggta
      961 gtagcaaaat atgatggagt atttgtagtt caccaaagaa gtgaagcaga tacaatttta
     1021 acttcaatgg aagaaatcat agaaattgga aaacaatcag gagtaaaagt tcatttttca
     1081 cattttaagg tttgtggtaa agcaaactgg aagtatatac cacaagttat agagctttta
     1141 gaaaaagcag aaaaggaagg aataagagta tcttttgacc aatatccata tgcagcagga
     1201 agtacaatgc ttggagtagt tttacctcct tgggcacatt ctggaggaac tgataagtta
     1261 atagaaagat taagtgatga aaacgaaaga gcaaaaatga aaaaagatat agcaaatgga
     1321 atagaaggct gggataattt tatagaattt gctggaatag accaaatatt tgtaacaagc
     1381 gtaaaaacag aaaagaataa agatacaatt ggtaagaact tattagaaat tggaaaaatg
     1441 agaggaaaag acccattaga tgcaacgttt gatttattaa aagaagaaga aaatgcagtt
     1501 gggatggtag atttctatgg attagaagaa catataatag gattcatgaa aagagacgag
     1561 caaaatgtgt gtacagatgg tcttttagca ggtaaaccac atccaagagc ttatggttct
     1621 ttccctaaaa tactaggaag atatgttaga gagttgaatg tgttaacaat cgaagaagct
     1681 gtttataaaa tgactaaaaa ggcagctaca agctttagta taaaagatag aggcgaatta
     1741 aaagaaggat attttgcaga tatagttata tttgacaagg atacagttag tggttgtgat
     1801 gattttatca actctatgca gtaccctaca ggtatagatt acgtaattat aaatggaaat
     1861 tgtgttgttg aagaaggaaa atacaataaa attaaagcag gaaaagtact taaaaattaa
     1921 atcttagctt taaggaggaa taaatttatg attttagtgg ggaatgggag gctaataagc
     1981 caagattctt taaatcccta tatggaagat ggatgtgtag taataaatga taatctaatt
     2041 gaagatatag gtactacaga agaaatgaaa gctaaatatc cagtacatga atttatagat
     2101 gcaggcaaga aaataatcat gccgggtcta ataaattcac atatgcatat atacagctca
     2161 tttgctagag gaatggcagt tccaggaaaa ccttctgaga actttttaga gatactaaac
     2221 aatttatggt ggagacttga caaacagctt acactggaag atacaaaata cagtgcatat
     2281 gcaacgtata tagaatgtat aaaaaatggt gtaacaacag tttatgacca tcatgcaagt
     2341 ccaaatgcaa ttgaaggcag tttgtttaca atatcagatg ttgcaaagga tttgggaatt
     2401 agaacgtgtc tttgttatga agtgtctgat agagatggtg gaaatacaat ccaacaaggt
     2461 atcaatgaaa acgtaaactt tataaaatac gctcaaaaag atgatacaga catgataaaa
     2521 ggaatgtttg ggcttcatgc tgcatttaca ctatctgatg atacattaaa taaatgtgct
     2581 tcagagatgg ctggtcttga tacaggttac catgtacatg ttgctgaagg actagcagac
     2641 ttacaatata atgtagataa atatggaaaa agggttttag aaagattcaa tgattttgga
     2701 gtactaaaag aaaaaacaat agcagttcat tgtatatata taagtgcaga agaaatcagt
     2761 cttttaaata aaacaaatac atttgtagtt cataatccag aatctaatat gggaaatgca
     2821 gttggatgtt ctcctgcaat agagttatta aaaaggggag ttacagtagg attaggaact
     2881 gatggatata ctagcgatat gtttgaatca ttaaaagttg ccaatataat tcacaaacat
     2941 cacttaaaag acccaagagt tggatttgaa gaatcacaaa caatgttatt tgataacaat
     3001 agaaaaatag ctaaagctta ttataaaaat gacctaggag tacttaaaaa aggtgcttat
     3061 gcagatgtta tagtagttga ttatatacca catactccat tgacagaaaa tacaataggg
     3121 ggacacacta tttttggaat gagtggaaga agcgtagata caactattat aaatggaaag
     3181 gtacttatga aagatagaaa gttaataaac atagatgaag aaagtattct agcaaaaagt
     3241 agagaattaa gtcaaaatct atggaataga atttaaataa ttgattatgc aaaattctat
     3301 gaagcaggaa ccctgtgact ttagtcatgg gaggttcaga ttaatataca agtaagaaac
     3361 aagaatttag atgttaaaaa cagagcttaa acaaaaatag gaatttaaat gaagtatata
     3421 aataaaaaat atacggaata gaatattaaa ttaaatatag aagggagacc caaaatgtct
     3481 cgagataaaa gccctaaaat agctccagta gatgaaaggt tacctgtatc caaatcttgg
     3541 gtattttcac tacagcacgt tttagcaatg tgtgcaggag cagtagcagt tcctatgatg
     3601 ataggaagtg ctgcacgact agatcatgct agtattgttt ttttaataaa tgcttgcttg
     3661 tttatggcag gtataggaac actcatacaa agtgttggta taggaaatat tgtaggtgct
     3721 aaaataccag taatagaagg aactagtttt gccgctgtgg caggtatgac agcaatagcc
     3781 tcatcatata gtgattcaca agttgcaata cagacagtat ttggctctgt aatagtagct
     3841 ggattgtttt gtatattact tgctccagta tttgaaaaat taataagatt tttccctagt
     3901 gtagttactg gaacagttgt tatggtaata ggtatttcac tatttccagt tgggataaaa
     3961 tggataactg acaacaaatt ggaagcggtt gaaccaaact cattagcatt agctttagca
     4021 gtattggcaa taactttact ttcatttaaa ttcttaaaag gaatttggaa tagcacagct
     4081 atattggtag gtatagtact tggtacacta ctttccatgg cagttggaat ggcagatttt
     4141 agtgcagtaa atggagttgg ttggcttaac ttaaatgtac ctttaaaatt tggaatgcct
     4201 agatttgaac catctgcaat actatcattg tgtttgatta tgttggtact tatgacggaa
     4261 tcagttggta atatgatagc tattcatgaa atggtagaca aagaagtaac aggtaaaaat
     4321 attaaaagag gtctatttgg agatggaata tctacagcat tatctggaat attcaattca
     4381 tttccaataa ctccatttgg tcaaaataca ggaatagtag gtcttacagg tataaaatca
     4441 agatttatag cagtttacgc aggggttata ttgctagtac ttggattctt accaaaattt
     4501 gcagcaataa taggagcaat acctaagcct gttcttggag gcgtaggatt tgcaatgttt
     4561 ggaatggtaa tggttggagg agtaaaaact ttaagcaaag tgaaatttga tggaactaaa
     4621 aatggggtta tagtagcagt tagtgttggt ttgtctatga taccactaac aaatcctgat
     4681 ttctatcata atttcccaac ttgggtacaa acaatattcc atagtggaat aacaacaggt
     4741 agtttatcag cagtgctttt aaacatattc ttcaatgaac taggttctaa aaaagaaaaa
     4801 aataataaag ttgaagaagc aaaataaaaa taaaacaagt aaaaatctat attgacttaa
     4861 agtcattaag tattaataaa aataattcag agatattaat aattcaacaa aactatctta
     4921 tattttaaat aatataaaaa agtaaataaa taacaaatac aaataaaaga atacaatcta
     4981 aatattaaaa atatttaatt taagggagag ataataatat ggctaattta aaagttaatt
     5041 tatatggaat ggaattttta aatccaatta tgacagcggc aggacctgga gcaaaagatg
     5101 gtgctctctg tcaagaggca gcaaaaggtg gagcaggagc tatatgtaca aaaactatat
     5161 ctgtattacc agctgatgta ccaagaccat gtatggcaaa aactaatagt ggttttttaa
     5221 acacagaatt atggtcagag cttccaaaag agcaatggat agaggaagaa tacaaaaagg
     5281 taaaagaaac agggcttcca gtaatagtaa gtatgggata ttcagcagag caaatagctg
     5341 aggttgctcc tttagtaaag ccttatgcag atgcagttga attatctact cattatgtag
     5401 gtacagacgt tgcaccaata gtaaatgctt taaaagctgc aaaagctgtt ttagatgtac
     5461 cagtatttat gaaaatgagt cctcatactg acattcaaac tattgcaaaa gctgttgaag
     5521 aagcaggtgc agatggactt gtaatgataa actcatttgg accatgtatg gcaatagatg
     5581 taaatacagg ataccctata atgggaagta aagctggata cggatggtta tcaggtgcac
     5641 caataagacc tctagcaata agatgtatat atgaagcttc tcaagttgta aatataccaa
     5701 taatcggtgt tggtggaatc acttgtggaa aagacgtggc tgaaatgatg atggctggag
     5761 caagtgctgt tcaagtatgt acagaagcaa tccttaaagg gccttctatc tatggaaaaa
     5821 ttgctaaaga attaaatgaa tttttagatg agaatggata tgctgatgtt aatgaaataa
     5881 aaggactaac tcataagaaa atggcagcta gagggtttag aactcattca gtagctccag
     5941 tagtggatga taacaagtgt atcaaatgta agcaatgtgt aacaagctgt gtatacgaag
     6001 ctttagaagt tacagataag ttgaacatag atgcagacaa gtgttttgga tgtggattat
     6061 gtgtaactag atgtccaaaa ggagctttaa gcatctgcta caatcaagta gaagctatga
     6121 atgagtaata attatataga attttggtaa ttaacttaga gtaataggta ttaggataat
     6181 aaaatatatc agataaacaa acacacatta tgcattaata atacagtcta aagttaataa
     6241 ttttattata ttgatttaac taaatattag tttagctgaa tattaattta attaggtatt
     6301 ggtttaatct taatatagat ttactgcgta taagtgaact aactagatta ctttaagtta
     6361 attttttcat ataaaataaa ttgatacatt acaagtaagg tggagtctat atgggaattg
     6421 ttttaaaagg tgggaaagta gtaacaagtg aaaaaactta ctgtgcagat gtaagaatag
     6481 aagatgaaaa aatagtcgat ataggtcttg atatatcaaa agaaaatgat gaaatagttg
     6541 atgttagtga ttcttatgtt ataccaggag gtatagatac acacacacac tttgacttag
     6601 atacaggcgt tactaagaca gcagataact ttgagacagg aacaaaagct gctatagtag
     6661 gaggaactac tactatattg gattttgcta cacaagctaa aggatgttct ttgaaagacg
     6721 gattagaaga atggcatgaa aaagctaagg ataaatccta ttcagattat ggctttcata
     6781 tggctataac agattggaat gactcggttt gtaatgaaat ggaggatatg gtgaaagagg
     6841 gagtatcttc atttaaactg tatatggcgt ataaaggaag ccttcaagtt gatgatggtg
     6901 taatctttga agcattgaga aaagcagaag agataggagg gattattggt ttccattgtg
     6961 aaaatgggga cataatatgt gaacttgtgg ataaagcaaa gtcagaaaat aatttatctc
     7021 caaaatatca tcaattaact agaccagcta taatggaggc agaagctaca tcaagactta
     7081 tgaagatagc tgaagttgca aactctaaag tatatgtagt tcacttaagc tgtaaagaat
     7141 ccttagagga ggttttaaaa gctaagcaaa gaggtgtgga tgtaatagtt gaaacttgtc
     7201 ctcaatacct tctattaaat gatgaattat atgatttaga tggatttgaa agtgctaagt
     7261 atgtaatgtc accaccttta agaaataaag aaaataatga aatattatgg aatgctattt
     7321 tagataatca aatagacact ataggaacag accattgttc ttttaattat aagggacaga
     7381 aggaacttgg gataaacgat ttcagtaaga taccaaatgg ttctccaggt gtagaacata
     7441 ggatgagtct tttatacaca tatggagtct gtaaaaataa aatatctatc aacaagatgg
     7501 tagaagtaac atcaactaat gcagcaaagt tgtttggaat gtatcctaat aaaggaacta
     7561 tagaaatagg cagtgatgca gatatattag tcttaaatcc aaacaaaaaa tccaaaatag
     7621 aagcaaaaaa acaattacaa aatgtagatt atacacctta tgaagggtat gaagtggatt
     7681 gtcaaataga acatgtttat ttaagaggaa aagaagtagt tagagatgga gaattaattt
     7741 gtaatactcc aagtgggaga tatataaata ggcgtctaac aaagggggct agagaatggt
     7801 agaatttata ttaaatggag agaaagttgt tgcaaaagaa ggcaaaaaac ttttagactt
     7861 ccttagagaa gatatgaata taacttcagt taaaaatgga tgttcagaag gtgcttgtgg
     7921 aacatgtatg gttataatag atggtaaagc tactaaagca tgtgtattta aaactgataa
     7981 attagctggg aaaaacataa caactataga gggattatca gagagagaaa gagatgtttt
     8041 tgcatattct tttgctagtc aaggagctgt acaatgtgga ttttgtattc caggtatggt
     8101 aattagttca aaagcactta tagacgttaa taataatcct actgaagatg atataaagaa
     8161 agctttaaaa ggtaatatat gtagatgtac agggtatgta aaaataatta aagctgtcga
     8221 tttagcggca aagattttta gagaaaatat agatgtgcca aaagtaaact gtaagggtct
     8281 agtaggtgaa aatcttccta gagttgatgc agttggcaaa gcacttggat attctgaata
     8341 tgtagatgat atgagaattg agggtatgat ttatggttca gcagttagag ctaagtatcc
     8401 tagagcttta gttaaaaaaa tagatataga agaagcaaaa gcttatccag gagttgttgc
     8461 agttctaaca gctaaagata ttccaggaac tataaaggta gggcacttaa agaaggattg
     8521 ggatacttta ataccagaag gtgaaataac tagatactta ggagatggaa tagttttagt
     8581 agcagcagaa actagagaag cgttagaaga ggccaaaaag ctagttaaga tagattatga
     8641 ggaattaaca ccattaagta ctacagcaga tgcacttaga gaagatgctc caaagataca
     8701 tgagacaggg aatatacttg ttaaggaaca tttagtaaga ggaaacgcta aagaaaagat
     8761 agaaaattca aaatatgttg taactaaaag atatactaca cctgctacag aacacgcatt
     8821 tttagagcct gaatgtgcag tagctggtcc ttatgaagat gatggagtaa taatctattc
     8881 gacagaccaa ggggtgtttg ccactcaaca tgaatgtgca gatatgttag ggttaccata
     8941 tgagaaggta agagttataa acaaaatggt aggaggaggg tttggtggaa aagaagacat
     9001 gagtgttcaa caccatgcag ccttacttgc ataccataca aagagatttg taaaagtaca
     9061 gttaacaaga aaagaaagta ttattgttca tccaaaacga catgccacag aaatggaatt
     9121 tacgacagca tgtgatgaaa atggatactt aacgggtatg aaggcaacga taatatctga
     9181 cacaggtgca tatgcctctc ttggaggacc agtgctacaa agactgtgta ctcatgcagc
     9241 aggaccatac aattatcaag atattgatat aacaggaaca gcagtatata ctaataaccc
     9301 tcctggagga gcatttagag gatttggagt tacacagagt tgttttgcaa ctgaatgtaa
     9361 tctaaattta cttgctgaaa tggttggaat atctccttgg gagataagat ataaaaatgc
     9421 aataagacct gggcaagttt taccaaatgg tcaatttgca gatgaaggaa cagcaatggc
     9481 agagacacta gaagctgtaa aagaagagta tgaaaagcat ccacgtgcag gtatagcttg
     9541 tgctatgaaa aatagtgggg ttggagttgg tatacctgat gttggaagat gtagattggt
     9601 gataaaagat ggcaaagttc acataagaac aagtgcttct tgtataggtc aagggcttgg
     9661 tactgttatg actcaagtag tttgtgaaac tgctaaccta cttcctgaaa atatagttca
     9721 tgaagttcca gatactcatt taacaccaga ctctggtaat actacggctt caagacagac
     9781 agtatttacg ggagaagcta ctaaggttgc atcaaataag ttaaaagaag ctttagactc
     9841 tggcaaaact ttacttgatt tagagggaga agagttttat ggagaatatg aaagtattac
     9901 agataaaatg ggttgtgaca aagaaaatcc agtaagtcat gttgcttatg gatatgcaac
     9961 acaagttgta attctggatg aaaatggaaa actagaaaaa gtagtagcag cacatgatgt
    10021 tggaagagct ataaatccaa tcagtttaga aggtcaaata gaaggtggag tagttatggg
    10081 tcttggatat gctctaaccg aagattaccc agtagttaac tctattccta cagctaagtt
    10141 tggtacatta ggtttattta aatctacaga tataccagaa atacaagctt taatagttga
    10201 aaagaacaaa gatttattgg cttatggggc aaaaggtgta ggagaaattt gtacagttcc
    10261 tactgcacca gcagtacaaa atgcctatta tgtatttgat aaagaattta gaactgagtt
    10321 accactacaa aatactccat atagtaaaaa aaagtaacaa aaaaaagaaa tattaaataa
    10381 aaaaatagat gaaattgtaa ataaaaaaaa cccttttgca taaggtatat tttaaatgat
    10441 agaatataca tatacaggag ggtttttctt ttataaaata agtttaataa aattgagagg
    10501 tgagcagaat gaattttaat aatagtctag aattaactca gtctcagaag ctaataatga
    10561 ctacacaact taagcaatca ttatcaatat taaatatgag taagttagag ttagaagaag
    10621 agataaaaag agaagcagag aataacccat tagttgaagt cgagaaaagt ggggagataa
    10681 attgggaaga gtatataaaa gacatggata ggtcaagacg tttagataga acagagataa
    10741 gctataatcc tgataatgaa gttaatctag aaaatttagt taaatattcg tcaaatttat
    10801 atgaagattt aaaatttcag ataagcctgt ataaacttac agacaaagaa ttggaagtct
    10861 gtgagtatat aattgatagt ttagatgagg atggatattt aagaactgag gaaaaagtaa
    10921 ttgttgatac cttaaagata gatgaagaac tttttgaaaa atgtttaatt agtgttcagc
    10981 agttggaacc tagtggagta ggggcaagaa gtctttcaga atgtctgatg atacaaatgg
    11041 ctagtttagg aatatataat gaaattttag aggaaatcgt tacaaaagac ttaaatttaa
    11101 ttgcaaataa taaatacaaa gagataagta aaaagtacaa tatgtcatta caaaaatgtg
    11161 ttgaactaat aaatataatt aagacattag accccaaacc aggaagaact tgttcagtag
    11221 agaaaagtgt atacattcaa cctgatgtaa cagtagaaaa aattgatgat gaatttattg
    11281 tatatttaaa tgagaaagat tcttatcaaa taagaatcaa tagttattat aaagacatat
    11341 taaaaaattc acagtctgat gaaagtgcaa aagaatttat aaaagaaaga ctaaattcag
    11401 caacaggact tatgaaaaat atagagagta ggaaaactac agtacttaag attgcagaag
    11461 agatagtaaa agctcaagat gaatttttga gaaaagggac aaaatatata aaacctttga
    11521 aaatgaaaga catagcagaa aaattagaat ttcatgaatc tactataagt agaggtgtaa
    11581 atggtaaata tatgctaaca ccttttggcg tatatgaatt taggtacttt ttcagtagcg
    11641 ccattgagac tgagaataat gaaatggcat ctagtacaag cataaaaaaa attataaagg
    11701 aaacaataaa agacgaaaat aaaaagaaac ctttaagtga tgaccatata tctaagttat
    11761 taaaagaaaa aggaatcaat gttgcaagaa ggacagttgc caaatatcgt gaagaattag
    11821 gaatcccatc ttcaagtaag agaaaagaat actaataagt aaaagacaaa tatcagtatg
    11881 gtaaggggaa aaggctaatt aagagatgaa tgtagattgg gctaaaaatt ttataaagta
    11941 aaataatata atatgaaaaa gtattaaaat gctataaggt attaaaaaac tggggaacat
    12001 taaaacatta agatagcaaa gagcataaaa aactaaagaa gattaaaaca ttaaagaaca
    12061 ttagagcttt gaggaatatt agaactttaa agaatattaa aatttataaa atataaaaat
    12121 actaaaaaga attgaaaaac tggggaacat taaaatattg taaaatgcta aaaaactaga
    12181 aaacataatt aaataagtgc ttataaagaa tgattaaatt aaataagtgt ttgtaaagaa
    12241 taattagata ttagaaaaag aagaatacct agataaataa actattatta aatttaataa
    12301 tcacaaaagg aaaacacgta gggtataaat aaaaatctta actagataaa agtaaactta
    12361 gttgtagata aaaataacta gaaataaaat tatttatagt aataaatatt agaatgaaaa
//
LOCUS       DAMJVK010000106        12334 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.110, whole genome shotgun sequence.
ACCESSION   DAMJVK010000106 DAMJVK010000000
VERSION     DAMJVK010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12334
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.110"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(22..2133)
                     /locus_tag="PC414_003131"
     CDS             complement(22..2133)
                     /locus_tag="PC414_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2465508.1"
                     /translation="MTINKLYSKLRAYNWKNYTTLFFCIILSVVLVTSYALIYFSPTI
                     QNILPTGGDSRKQATMIFSVAILGCAVFTTYASSLFLKFKSKEIGIFMALGTDKKKLK
                     KLLFSEILLISIVCCFLGLILSIPVSFGIWKIFQLVIIDTREMTYHFGTQGFIYGFIF
                     SVFVTLCIFILSVKFINRTNIIDILNDQRISEPIQDVKDWYGILGIIFIILGIFLGYL
                     VPSLTINKLNYKLPSIWSFTYLLSLIGIYMIMTYIVVHAKRGKHPEKYYKNIISTSMM
                     RFMGRQTVKNMCVISFLIAGALFAVFYVPNMVTGIYDTLKNNPIDYTFTYNQRDKQVS
                     KKDIEQLADKHNIKIQNYMEIPSIELIVDGVESIYHKDGTIEKVYMKKSSYDMFFNSK
                     DFSKLIGKPITIKNGEYLAVVNKGNENQDYKYTKFISSPVTEKEIPFRYAGKIIFNGS
                     FFQNRSMNAYVLNEEDYKSFTKDLSIKNYNNSILFNVDKDTYAFANDLKKEIIKRTSK
                     EMGMFSGYDEYVKKRYESTGRKYFMDEEYPSGEGHLKLDPNNNQLYLNWKYYPNFKIL
                     QSQDMIKNMAVFLMLFIYVAIICFTAVGIIAYTRGVTIAINHKQVFMDLNRLGANNDY
                     IKFCIRNQLKKVFSYPLIAGSFLIYLFNFMIMLNNDGRIVNSEVNALLINLGFIGICG
                     VYMYLIYLLTYKKFKKIIGIL"
     gene            complement(2123..2875)
                     /locus_tag="PC414_003132"
     CDS             complement(2123..2875)
                     /locus_tag="PC414_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2465509.1"
                     /translation="MLKVNQLCKSYKTGSKTYPVLKNISFEINKGEFVAIMGSSGSGK
                     TTLLNCISCFIPIDNGEIILGNNNIRNLKEKELSEVRNQQLGFVFQDFMLFDGLSVFE
                     NICVPQIIKGSQVLQMENRAINLCELFGIEHIRKKYPAEISGGEKQRTAVARALMNNP
                     DLILADEPTGNLDSKSCRTVIESFLKAKQELDATILMVTHDSFAASFCDRVIVLKDGI
                     IYKEVVKSNSRHKFLDELLEVLKEMGGAQDDN"
     gene            complement(4141..4317)
                     /locus_tag="PC414_003133"
     CDS             complement(4141..4317)
                     /locus_tag="PC414_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465510.1"
                     /translation="MFFTFIGVISAILLLCTIICGLWIKLNNRNNDSNSHFTFSILSI
                     AFSLLIVVAFLVRN"
     gene            complement(4421..5029)
                     /locus_tag="PC414_003134"
     CDS             complement(4421..5029)
                     /locus_tag="PC414_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2465511.1"
                     /translation="MEKLLRGKIMKADSTKEQLINATIQLLSSHRDVSSITAREIVAK
                     ANVNLAMINYYFKSKEELINISIKKFLKRHMDEYKATHDTKNLAPKQQLREILIAFFD
                     FIGEHSQFIKISVPYILIQEEIVYPFEILPLIKAHYENQNNKKTEAEYRTIAYQITSF
                     IQLLFLRSNAFFKYSGIDILNIEQRNGLIDFQLDLLLVDITK"
     gene            complement(5128..5952)
                     /locus_tag="PC414_003135"
     CDS             complement(5128..5952)
                     /locus_tag="PC414_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2465512.1"
                     /translation="MFKIGEVSKLTQISIRMLRYYDELGILKPAKTDKYTGHRLYSVE
                     QISILQRIVLLRDSKFSVAEIANIVHNWNDEFVIKELNRKKNEIQKEIKQEQQRINKI
                     DKFIEAINCDKDEIHYNVVFKKIPSYKIISLREIIPDYQSEGILWEKLSKFIKEEHIE
                     VSRQSNNNIAFYHDEEVKDNGVDIEVGMVVKKIGKNKSGFIYRETEEIDMMACTMVYG
                     PYENIAGAYESFCYWLDKNSDYQISGINRQIGHKGEHNEINPENYLTEIQIPLIKV"
     gene            complement(6130..7023)
                     /locus_tag="PC414_003136"
     CDS             complement(6130..7023)
                     /locus_tag="PC414_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF2465513.1"
                     /translation="MQLYNTPFNTNKHLIYFFTFTLVLNWIFSLTPALLGITNTILGE
                     IIFYLAYTSPAIVGLFFISTIYPQSAKQDFIYRYLSFKEIGIKWILLTILFFALTFIT
                     SLIIGNYFNVKTSDIDWIHIAVFRPSKIAYMLLASFILSILSQEAGWRGYAIDKLLVR
                     FGFIGSSIILGLVCGIWYLGSYFTPNQIPYNLAQYSLFDAFLFIPNIVLLNFIINFVY
                     INTSRSILSAGLVHMMYYFFNIQLLLHYPIKLCVIAQYVQIIFGLIFLVYMISSNKFK
                     QKVNSEIEQIKSDDFEFELDW"
     gene            complement(7280..7717)
                     /locus_tag="PC414_003137"
     CDS             complement(7280..7717)
                     /locus_tag="PC414_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HDF2465514.1"
                     /translation="MKIHVEQGINCDDIEITIKCSALDNNIEKIISLLSIPLLDIKGK
                     KSGEIYKLNVCDILYFETVGNKTFAYYEDDVFEIDLKIYKLSKLLANTSFSRISKSMI
                     LNMDYIKSIKVISHNRMQATLKNNEKVIINRRYIKNIKEKLNI"
     gene            complement(7974..8123)
                     /locus_tag="PC414_003138"
     CDS             complement(7974..8123)
                     /locus_tag="PC414_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvrJ family protein"
                     /protein_id="HDF2465515.1"
                     /translation="MDDSLQLLITNVGFPIALSMYLLVRIEGKLNTLSNSIDTLSKNI
                     LIFKK"
     gene            complement(8136..8366)
                     /locus_tag="PC414_003139"
     CDS             complement(8136..8366)
                     /locus_tag="PC414_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HDF2465516.1"
                     /translation="MEIKKKLVMSFKNVKDKQVSFSIENPKDDLTEENIKSVMDLIVS
                     KNIFSVGGFDLASVVEAKVVETNTTPYDLVIE"
     gene            complement(9225..9446)
                     /locus_tag="PC414_003140"
     CDS             complement(9225..9446)
                     /locus_tag="PC414_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HDF2465517.1"
                     /translation="MEVVSTKNLSSIKLKLDAGFDDKGKAVVKSKSFANVKAEALNED
                     VYAVAEAIASLQENPIVDILKLDSTSLSK"
     gene            10026..10283
                     /locus_tag="PC414_003141"
     CDS             10026..10283
                     /locus_tag="PC414_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF2465518.1"
                     /translation="MKIKVEDIPVQFHAMVEIIGIDKFIEVAKLYGGTNTYIPTYKGL
                     FRYARNRDIVKQFNGVNHNELAIRYNMCVSNIKRILNENSV"
     gene            10752..11990
                     /locus_tag="PC414_003142"
     CDS             10752..11990
                     /locus_tag="PC414_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HDF2465519.1"
                     /translation="MNKGFYKLNSVVLNFDIKNDVNKIRFNHYIMRKENIQRLQGTLP
                     NGQFYMTVRRAATDLQLSISTISRLVKEFLDLGIIRLVSKGVKGNRSVYSYVSIEFNG
                     SLFDDSKYMELDCVCNDEFIDNSGIEFDCDSKDNSFNGDINVETVHCINEYFSDDDVE
                     HNYNNFHNKDTIKDKAISNISTIKKYIDESFDTNKSENSNLQQESINFNFNDTCNEID
                     KRTNNSIENNQNKRPMRLSDISLLSDKGMTNKKNYFNHNHGIQKGTKSVTKKKESLNK
                     FNKKNIQQSIINMLNKKSGKNFKIDSPISIKLINERLKEGYVLEDFYKVIEVKVAKWK
                     NTIMEMYIRPETLFSYKFESYVNENISMKSQSNVHPCKEGNSNDSSNYIHSDYITYDG
                     SVRERKKYENVRYWNPICEI"
BASE COUNT         4579 a         1458 c         1672 g         4625 t
ORIGIN      
        1 aaaatctaat aacctttttt attataaaat accaataatt tttttgaatt tcttataagt
       61 taatagatat ataaggtaca tataaacccc acaaatccct ataaaaccta agtttatcaa
      121 aagagcattg acctcactat tgacgattct accatcatta tttaacataa tcataaaatt
      181 aaacagataa attaaaaaac ttcctgcaat aagagggtat gaaaatactt tctttaattg
      241 atttctaata cagaacttta tataatcatt gtttgctcct aatcgattta aatccataaa
      301 gacctgcttg tgatttatag ctatagtaac accccttgta tatgcaatga ttcctactgc
      361 tgtaaaacat atgattgcca catatatgaa taacattaag aagactgcca tgttttttat
      421 catatcttgg ctttgtaaaa ttttaaagtt aggatagtat ttccaattaa gatacaactg
      481 attgttgttt gggtctagtt ttaaatgacc ttcaccactt ggatattctt catccataaa
      541 atattttctt ccagttgatt cataacgttt ttttacgtat tcatcataac cactgaacat
      601 gcccatttcc ttagatgttc tttttataat ttctttcttt aagtcattgg caaaagcata
      661 ggtatcttta tctacattaa atagtataga attattgtaa ttttttatgg ataaatcttt
      721 agtaaatgat ttataatcct cctcattaag gacataggcg ttcatacttc tattttgaaa
      781 aaaagaacca ttaaatatta tttttccagc atatctaaag gggatttctt tttctgtgac
      841 tgggcttgat ataaatttgg tatatttata atcttgattt tcatttcctt tatttacaac
      901 agcaagatat tcaccatttt ttatagttat tggtttgcca attagcttgg aaaaatcttt
      961 ggaattaaag aacatatcat aggagctttt tttcatatat accttttcga tagttccatc
     1021 tttatgatat attgattcta caccatcaac aattaattct atagaaggaa tttccatata
     1081 gttttgaatt tttatattgt gtttatctgc taattgttca atatcttttt ttgatacctg
     1141 tttatctctt tgattgtagg taaaagtata atctataggg ttatttttaa gagtatcata
     1201 gatacctgta accatattag gaacatagaa tacagcaaat aatgctcctg caatcaaaaa
     1261 tgaaataaca cacatgtttt ttactgtttg tcttcccata aatctcatca tgctagttga
     1321 tatgatgttt ttatagtatt tctctggatg ttttcctctt ttagcatgta caactatgta
     1381 tgtcataatc atatatattc caataagaga gagtagataa gtaaagctcc atatagatgg
     1441 tagtttataa tttaatttgt ttatagttag tgaaggtacg agatatccta gaaaaatacc
     1501 gagtataata aaaattatac ctaatatacc ataccagtcc tttacatcct gtattggttc
     1561 agatattctc tggtcattta atatatcaat tatatttgta cgattaataa attttacaga
     1621 aagtataaat atgcataatg tcacaaatac actaaatata aatccatata tgaatccttg
     1681 agttccaaaa tgatatgtca tttctctggt atcaataatc actaattgaa atattttcca
     1741 tataccaaaa gatacaggaa tacttaatat aagacctaaa aaacagcata ctattgaaat
     1801 taacaatatc tctgaaaata gaagtttttt taatttcttc ttatctgttc ctagagccat
     1861 gaaaatacca atttccttgc ttttaaattt taaaaagagg gaagaagcgt atgtagtaaa
     1921 gacagcgcat cctaatatag caactgagaa tatcattgta gcctgttttc tgctatctcc
     1981 accagttgga agtatgtttt gtattgttgg gctaaaataa attagagcat atgaagttac
     2041 taataccaca gaaagtatta tacaaaagaa taaagttgta tagttcttcc aattataggc
     2101 tcgaagttta ctgtataatt tattaattgt catcttgagc gcctcccatt tcttttaaaa
     2161 cttctagtaa ttcatctagg aatttatgtc ttgaatttga ttttacaact tctttataaa
     2221 ttattccatc tttaagcaca ataactcggt cacagaagct tgctgcaaag ctatcgtgtg
     2281 taaccatgag tatagtagca tctaattctt gctttgcttt taaaaatgat tcaatgactg
     2341 tacgacaaga cttactatca aggttaccag ttggttcatc tgctaatatt aaatctgggt
     2401 tgttcatgag tgctcttgct actgctgtac gctgtttttc tccaccagat atttcggcag
     2461 ggtatttttt tctgatatgt tcaataccaa atagctcaca tagattaatt gcacggtttt
     2521 ccatttgtaa gacttgacta cctttgataa tttgtggaac acatatattt tcaaagacac
     2581 taagaccatc aaatagcatg aagtcttgga atacaaatcc tagttgttga tttctaactt
     2641 ctgaaagttc cttttctttt aaatttctta tattattatt tccaagaatt atttccccat
     2701 tatcaattgg gataaaacat gaaatacaat ttaaaagagt agtttttcca cttcctgatg
     2761 aacccatgat tgcgacaaat tctcctttgt tgatttcaaa agaaatattt tttaatacag
     2821 gatatgtttt actgcctgtt ttatatgatt tacataattg gtttacttta agcatatata
     2881 tcatcctcac tttcatttat cttgtaagtc aatgatagca tttgaaaggg gaggagggat
     2941 atgagctata ttgtaatagt tggatgtttt tttgtaatgt ttgaaaatat attgtttgga
     3001 tagtgtgaga ggttattatt atagttaaat tgattatatt attggaggat ttagagggtt
     3061 atttttaggt attaattgtt gtgctaaagt aatatgatgt ggaacattga aggtgtaatt
     3121 agaaaagaag tatatagtaa aagtaaaaag gttatagaat aacgaatgtt gtattgaaaa
     3181 aaatgatgta aataatgtat agtagggtta aaaagaaaaa aagaattata gagcagtaag
     3241 attatttaat ggaatgtatt gtaaataatt tgaaaggatt taggggctat taacacaatg
     3301 taattgagaa aatatattgg agttttggaa ataaataggt tgttagaaac aattgaaatg
     3361 tataataaaa aggttaaaaa gtattattag tataggtatg atagataatt ttagaaagtg
     3421 gaagtatttt agactatgct gaaaagtgat tgatatgatt ttgagatatt aaaatattac
     3481 tatattatag taaagaagtt aaaataataa aatgttgtaa taataaaggt ttgtagtata
     3541 ataaagaact gtaataaata agactttgca atgtaataga aatgctgtaa taataaaagt
     3601 ctgtagtata ataaagaact gtaataaata agactttgca atgtaataga aatgctgtaa
     3661 taataaaggt ctgtagtata gcaaagaact gtaataaata agagattgta ataataaaag
     3721 tctgtagtgt aataaagaac tgtaataaat aagactttac aatgtaatag aaatgctgta
     3781 ataataaagg tctgtagtat aataaagaac tgtaataaat aagactttac aatataatag
     3841 aaatgttgca ataacaaaac accataataa ataagagttt gcaatataat agaaacactg
     3901 caataaataa gagatttcaa tataataaaa gtctgtaata taacaaaatc ctataataaa
     3961 taagatttta caatataata aaaatcctat aataaatagg actctgtaat ataataaaat
     4021 gctgtaacaa caaaacacta taataaataa gaccctgtaa tataataaaa atactataat
     4081 atagtaaaaa tcatgcaata ataaaaatct ataataataa acctcagcat aacaaagaca
     4141 ttaatttctt actaaaaagg ccaccacaat taacagtgaa aaagcaattg aaagtattga
     4201 aaaagtaaag tgagaattag agtcattatt acgattatta agttttatcc ataaaccaca
     4261 tataattgta cacaacaata aaattgctga tattactcct ataaatgtaa aaaacattaa
     4321 aattcctccc ccttatttaa tgcaagtatt tttagtcaat gagaatattt tcaaacgtta
     4381 ccataaaata aaaaaatgta cataaaaaat taatatagta ttattttgta atatctacca
     4441 ataataaatc taattgaaaa tctattaaac catttctctg ttcaatatta agaatatcaa
     4501 taccactata cttgaaaaaa gcattagaac gaagaaaaag caactgaata aatgaagtta
     4561 tttgatacgc aatagttctg tattcagctt cagttttttt gttattctga ttttcatagt
     4621 gagccttaat taaaggtagt atctcaaatg gataaacaat ttcttcctgt atcaatatat
     4681 agggtacaga aatctttata aattgactat gttcacctat aaaatcaaaa aatgcaatta
     4741 gtatttccct taattgctgt ttaggtgcta aatttttagt atcatgtgta gccttatatt
     4801 catccatatg acgctttaaa aattttttaa tagaaatatt aattaactcc tctttagatt
     4861 taaaataata attaatcata gctaaattga catttgcttt agctacaatt tctctagccg
     4921 taatgcttga aacatctcta tgtgaagata gtaactggat tgtggcatta attaattgtt
     4981 cctttgttga atctgctttc ataatttttc cccttaaaag tttctccata aaaaaattaa
     5041 acatgtttaa tacacgttta gatactatca tgaaaatttt gggatgtcaa tgggtgatta
     5101 gtacttaagt atttaagtac taatctatta gacttttata agggggattt gaatttctgt
     5161 aagataattt tcaggattta tttcattatg ttcaccttta tgtccgattt gtctatttat
     5221 acctgatatt tgataatcac tatttttatc taaccaataa cagaacgatt catatgcacc
     5281 agctatattt tcatatggac cataaaccat agtacatgcc atcatatcaa tttcttctgt
     5341 ctcacgatat ataaatccag atttgttctt tccaatcttc tttacaacca ttccaacttc
     5401 aatatctaca ccattatctt tgacttcttc atcatggtaa aaagctatgt tattgttgga
     5461 ttgacgtgat acttctatat gttcttcttt gataaattta gatagctttt cccagagtat
     5521 accttcagat tggtagtctg gaattatctc tctaagtgat atgattttat aacttggaat
     5581 ctttttaaag acaacattgt aatgaatttc atctttatca cagtttatag cttctataaa
     5641 tttatcaatt ttatttattc tttgttgttc ctgctttatt tccttttgta tttcattctt
     5701 ttttctattc aattccttaa ttacaaattc atcattccaa ttatgtacga tatttgctat
     5761 ttctgcaacg ctaaattttg aatctctcaa taatacaatt ctttgtaata ttgaaatctg
     5821 ttcaactgag tataatctat gacctgtata tttgtctgtt tttgcaggtt ttaaaatacc
     5881 aagttcatca taataacgca acatacgaat agaaatttgt gttagttttg aaacctctcc
     5941 tattttaaac atgaaaatat gacctccttt tcacttataa tcagtatata ttttgttttt
     6001 atatacttca agcgtttgtg gaaatacaat tattttccag atgttgcaat aaattataga
     6061 gagtaaatta tatttaagta gtaatcgtat caaatcatag attatcttaa tttataattc
     6121 atattaaaat taccagtcta actcaaattc aaaatcatcc gactttatct gttcaatttc
     6181 agaatttacc ttctgtttaa atttattaga agaaatcata tatacaagaa atattaaacc
     6241 aaatattatt tgtacatatt gagctattac acataattta atagggtagt gtagaagtaa
     6301 ttgaatgttg aaaaaatagt acatcatatg aactaatcca gctgataaaa tgcttctact
     6361 tgtattgata tatacaaaat taattataaa gtttaaaagt acaatgtttg gtataaataa
     6421 aaaagcatca aatagggagt attgtgccaa attatatggt atttgattag gtgtaaaata
     6481 cgaccctaga taccagattc cacaaacaag ccctaaaata attgaagagc ctataaatcc
     6541 aaatctaact aataatttgt caatggcata tcctctccat ccagcttctt gactaagtat
     6601 actcaatata aaacttgcta atagcatata agctatttta gaaggtctaa acactgctat
     6661 gtgtatccaa tctatatcag aagtttttac attaaagtaa tttccgatta ttaacgaagt
     6721 aataaaagtt aaagcaaaaa ataatattgt taatagtatc cattttattc cgatttcttt
     6781 aaagcttagg tatctataaa taaaatcttg ttttgcactt tgtggataga ttgtagaaat
     6841 aaaaaataga cctacaatag ctggtgatgt atatgcaaga taaaaaatga tttctccaag
     6901 tattgtattt gtaattccga gcaacgcagg tgtaagactg aatatccagt tcaatactag
     6961 agtaaatgta aagaaataga ttaagtgttt gtttgtatta aagggggtgt tatatagttg
     7021 cattttttca ctatccttcc gttttataat gaagttttaa tttttattaa ttgtttattt
     7081 atttttatat gtattaagtt atcgcttgat gttagttatt gcctataata atatcctatt
     7141 ttttatattt tgattaatta tttttacact aaatataatt aattagtatt taatattgaa
     7201 tatattatat tattattaat tattaaatag gtcaataaat ttgcaattag tgatattaat
     7261 aaattacttt agatttttat taaatgttta gcttttcttt aatatttttt atataacgac
     7321 gatttataat aactttttcg ttgtttttta aagtagcttg catgcgatta tgtgatatta
     7381 cttttatact ttttatgtag tccatattta aaatcatgga tttagatatt cgtgagaaag
     7441 atgtattagc taataatttt gataatttat atatttttaa atcaatttca aatacatcat
     7501 cttcataata agcaaatgtt ttatttccaa ctgtctcaaa gtataaaata tcacagacat
     7561 tgagtttata aatctctcct gattttttac cttttatatc aagtaacggt atactcaata
     7621 atgaaattat tttttcaatg ttattatcta gagcagaaca ttttatggtt atttcaatat
     7681 catcacaatt aataccttgt tcgacatgta ttttcattaa tcatcacctc attttattac
     7741 tgtcattaga atagcattat atcttattga gtccaactag atgcgttcta aatttcaaat
     7801 taagcatagt gaaattcaaa aataaaacag tgaaattcaa atttgaggtg gtaaaattca
     7861 aaaaaatact atggatattt ggataaatta tccaaatatc ctagtatttc acatagaata
     7921 tgtctaatta ttttacaaat agacctattt tatgacttac atatattgtc aaattacttc
     7981 ttaaatatca aaatattttt ggataatgta tcaatactgt ttgataaagt atttaattta
     8041 ccctcaattc taaccaacaa gtacatggat agagctatag gaaaaccaac attggttatt
     8101 aacagttgta aactatcatc cataaaatta gccccctatt ctataactaa gtcataagga
     8161 gtggtatttg tttccacaac cttggcttca acaacagaag ctaggtcaaa tcctccaacc
     8221 gaaaaaatat ttttagatac tatcaaatcc atgacagatt ttatattttc ttcagttaaa
     8281 tcatcctttg gattttctat agaaaaagaa acttgtttat cctttacatt tttaaagctc
     8341 atgactaatt ttttcttaat ttccatataa ttacctccga tataataatt tatattttaa
     8401 ttgtttgctt gttaatattg gtattttaat tatcaatatt aatgttttgc ttgttagtat
     8461 taatatttca attgtcaata ttaatgtttt gcttgttagt attaatattt caattgtcaa
     8521 tattagtgtc ttgcttgtta gtatcagtgt ttcaattgtc aatattagtg ttttgcttgt
     8581 tagtattaat atttcaattg tcaatattag tgttttgctt gttagtatta atatttcaat
     8641 tgtcaatatt agtgtcttgc ttgttagtat taatatttca attgtcaata ttagtgtttt
     8701 gcttgttagt attagtattt caattgtcaa tattagtgtt ttgtttgtta gtatcagtgt
     8761 ttcaattgtc aatattagtg ttttgcttgt tagtattagt atttcaattg tcaatattag
     8821 tgttttgctt gttagtatta gtatttcaat tgtcaatatt aatgttttgc ttgttagtat
     8881 tagtatttca attgtcaata ttagtgtttt gcttgttagt atcagtgttt caattgtcaa
     8941 tattagtatt ttaattgttg actctagtta ttgctgatat agaaaatatt taatttattt
     9001 caattaatta gattaaagta gggtagcatc taaaatagct aacatgttta agttgaaata
     9061 cgtttaatat attaattttt caaagcgtta atatattaaa gttataacca aaattgaagt
     9121 tataatctgc caaatctata gcgtattaag tgcattatat aacttaatct tttatataaa
     9181 gagttaattt tttaatttgt aaagcagagt acgtttaaaa gacactattt agatagtgat
     9241 gtactatcta attttaaaat atctacaata gggttctctt gaagacttgc tattgcttct
     9301 gcaacagcat aaacatcttc atttaatgcc tctgctttta catttgcaaa tgatttactt
     9361 ttaacaactg ccttaccctt atcatcaaaa ccagcatcca attttagttt tatactagat
     9421 aaattttttg ttgaaacaac ttccataaaa ttacctccta aaaatttaat ttcaaagatt
     9481 tgtcgacttt ctttgttagc tataatacta tcatctaaga taatatcgga gttaaacagt
     9541 aatttctaaa gattttttgt gtaaattttt ataggaaaaa ttttactttt ttgctgatga
     9601 aaaagtttac aagttatttg tgaagatatg ttcttagtgt tttgaaatag tatttgtata
     9661 tattcatgct tagttgagat agttttgtat ggattttcat atgtgtagag ttaaatgtta
     9721 ggtactaaag tttatgttaa attaaatgtt aaagcttatg ttaaaaattt tattaaattt
     9781 aatctgtaaa taaaaaagtt aaaagtccaa tattaacatt ttacttttaa accattaaaa
     9841 tctttattaa agctaacaaa gttataagtt atgagaaatt ttattgaagt taatacaaaa
     9901 attaatttta aagctaagaa attttttatt agattcaata aaactaataa atatagttta
     9961 gtcaataatg tattaaataa aaactttgtt aagtctataa aaatatctta aaggaggtgt
    10021 aacttatgaa gataaaagtg gaggatatac cagttcaatt tcatgcaatg gtagaaataa
    10081 tcggaattga taagttcata gaggttgcca agttgtatgg aggaacaaat acttatatac
    10141 ctacgtataa ggggcttttt agatatgcta gaaataggga tatagtaaaa caatttaatg
    10201 gagtcaacca caatgaattg gctattagat ataatatgtg tgtaagcaat attaagcgaa
    10261 tactcaatga aaatagtgtt taaatataat aaaataatgt ttaaacataa tgaaaatagc
    10321 cttgttaaac gtaagagtaa agacaaagaa gatgttttat aaagtttttt tagacaaatt
    10381 aactaaattt actacttatt aatttaaata cattgtatat tctataaaaa aatttgttaa
    10441 atactggaga tatattgaga atacaaagaa aatggtcgaa aatatttgtt ggctaaaaaa
    10501 tagataaaat gatacaattg cttgtataaa ggggaaaaat aaataaataa actatataaa
    10561 taaagaaata gcaattaata tgacataata tatttataaa attaagaata taaatagata
    10621 ttttaaggga ttagttgtta aattaggaaa tattaaaaaa aatatatatt tatatttaaa
    10681 ctattttgtt atttatataa aaatacttat ctagttacta gcaattatta gtagaaatgg
    10741 gggaaataga gatgaataag ggattttaca aattaaattc agtggtattg aactttgata
    10801 ttaagaatga tgttaacaag ataagattta atcattatat aatgagaaaa gaaaacatac
    10861 aaagacttca aggaactctg ccaaacggac aattttatat gacagtgaga agagctgcta
    10921 cagatttaca actttctatt tctactataa gtaggcttgt aaaagagttt ttggatttag
    10981 ggataataag attagtgtct aagggtgtaa aaggtaatcg ttctgtatat agttacgtaa
    11041 gtatagagtt taatggaagt ttatttgatg atagtaaata tatggaactt gactgtgtat
    11101 gtaatgatga atttattgat aatagtggca tagagtttga ttgtgatagc aaagataatt
    11161 cttttaatgg agatataaat gtagaaactg tgcattgcat taatgagtat ttctctgatg
    11221 atgatgtaga gcataattat aacaatttcc ataacaaaga tactataaag gataaagcca
    11281 tttctaatat tagcactatc aaaaaatata ttgatgaatc ttttgataca aataaatcag
    11341 aaaattctaa cttacaacag gagtcaataa atttcaactt taatgataca tgcaatgaaa
    11401 tagataaaag aacgaataat agtatagaaa ataatcaaaa taaaaggcct atgagattga
    11461 gtgacatcag tcttttaagt gataaaggta tgacaaataa aaagaattac tttaatcata
    11521 atcatggtat acaaaaaggg actaaatctg taacaaagaa aaaagaatcc ttaaataaat
    11581 ttaataaaaa aaatatacag caaagcatta taaatatgct aaataaaaaa tcaggtaaga
    11641 attttaaaat cgattctcca ataagcataa aacttatcaa tgaaaggcta aaggaaggct
    11701 atgtcttaga agatttctac aaagttattg aggtaaaagt ggctaagtgg aaaaatacca
    11761 taatggaaat gtatataaga ccagaaactt tgtttagcta taagtttgaa tcctatgtaa
    11821 atgaaaacat atctatgaaa agtcaaagta atgttcaccc atgtaaagaa ggcaatagta
    11881 atgatagcag taattatata cattctgatt atataacata tgatggcagt gtgcgtgaga
    11941 gaaaaaaata tgagaatgta agatactgga atcctatatg tgaaatttaa ttagtttaaa
    12001 aatgtattta tgataaataa gatattaata gagaatagtg agctatagca aatgatatat
    12061 caatagttgg tagtaagtta tattaagtaa gatattacta tcaggtagtg aattatattg
    12121 aacgatatat taataattgg cagtaagtta tatcaaataa gatattacta tagagtaatg
    12181 aattatattg aacgatatat taataattgg cagtaaatta tattaaataa gatattacta
    12241 tagagtaatg aattatattg gacgatatat caatagttgg tagtaagtta tattaagcaa
    12301 gatattacta tcaggtagtg aattatattg aacg
//
LOCUS       DAMJVK010000107        11886 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.112, whole genome shotgun sequence.
ACCESSION   DAMJVK010000107 DAMJVK010000000
VERSION     DAMJVK010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11886
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.112"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            232..1113
                     /locus_tag="PC414_003143"
     CDS             232..1113
                     /locus_tag="PC414_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HDF2465520.1"
                     /translation="MVKKRKKKTVTLTILVVIVIGIFSMAWADMSRKKEMKETQERLK
                     AERLEKERIEKEKNEPIIGAKKEAVKEYGYDAKIVWDKLNKYDYSNNGEKIVFLTFDD
                     GPSTTNTPQVLDILKRHGVRGTFFIKGDSLERKGANEILKRTFDEGNAIAHHSYTHDY
                     KKLYPNRSLNLDTFVNELNKTDEAMKKVLGKNFSSNVVRCPGGYMSWKNMEPLGNYLK
                     EKNMASIDWNALNADAEGKKKNAQELFEHAKKSSEGKEMVVLLMHDTYGKQETVNALD
                     QIITYFKDNGYQFKILI"
     gene            complement(1174..2382)
                     /locus_tag="PC414_003144"
     CDS             complement(1174..2382)
                     /locus_tag="PC414_003144"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HDF2465521.1"
                     /translation="MKSIDEQMRIIMKGVDDLIDEKELREKLIKSEKEGKPMIVKLGL
                     DPSAPDIHLGHTVVLRKMKQLQDLGHQIVIIIGDFTGKIGDPTGKSKARKALTTEQVL
                     ANAKTYEEQIFKVLDKEKTIVRFNSEWLAKLNFEDVIKLAATITVARMLEREDFKKRY
                     EGQMPISVHEFFYPLMQAYDSIALEADIELGGTDQRFNLLMGRSLQREFGMESQIVIM
                     MPLIEGLDGKEKMSKSLGNYIGIDEEAGIMYQKSMEIPDELIIKYYNLVTDVHPDEVN
                     KIESQLKEGSVNPRDIKMNLAREIVTLYHGEESAKEAEERFKSVFQKGQIPEDIQTIQ
                     VKEDGFDLIEVLVSNEIVKSKSEVRRLASQGGVKVNGEKVEDLSTIVKESELVVQIGK
                     KKFVKIELVK"
     misc_binding    complement(2455..2707)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(2859..3848)
                     /locus_tag="PC414_003145"
     CDS             complement(2859..3848)
                     /locus_tag="PC414_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HDF2465522.1"
                     /translation="MIYGGSVLNTTKKLTEAALLSSLFIVVTIIAVSTGFGYAIYLDF
                     IVPVFFCIICLKCDLKYTILSGITSLLIISLVLGNLGTAIWASQSVLLGIMCGYLINK
                     PTMVMDDLVYGSVFGLIVMVFIDIYASTLIGYSFMKEFQGYSKWIYFNGYTNFIYYLM
                     IALFPFGMVFCIYLLSLILGNKLHILDSNSLKKFLIFKNFRSLNQFLCCSKKAFYICV
                     SYLAIFEVLKLLNVKVDSVYLKTIFISITYISLYFIIRDSCMSLQNFIIAKFRKLLYA
                     RILFLIIILLLFFIFDVTVIGLIIINAFLNKKINIRLSQSNIVNKQINLLIEK"
     gene            4000..4440
                     /locus_tag="PC414_003146"
     CDS             4000..4440
                     /locus_tag="PC414_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2465523.1"
                     /translation="MFKIKHFNDLSLDEFYEIAKSRYEVFACEQKIFSLNDYDDIDKN
                     SYHIFLKENGLICAYARIIPKEYSSYNDVSIGRVLVLSSHRRKGLAKQMMDCAIDFIK
                     VNLHENNITLSAQTYIKNLYLSCGFKEISEVYDEAGIEHIKMRL"
     gene            4767..4994
                     /locus_tag="PC414_003147"
     CDS             4767..4994
                     /locus_tag="PC414_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDF2465524.1"
                     /translation="MKNLNDIQVKKTVKVEDILSSGNLRERMLALGLTRGAVIDVVRK
                     GPKNNLTVYKIRGSKIALRQEESSLILVSDI"
     gene            5020..7140
                     /gene="feoB"
                     /locus_tag="PC414_003148"
     CDS             5020..7140
                     /gene="feoB"
                     /locus_tag="PC414_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HDF2465525.1"
                     /translation="MGLTHNSTKMSSLKDMFDIDNKEDQFVIALAGNPNTGKSTVFNH
                     LTGLRQHTGNWPGKTVATARGNFKYKNTEYALIDLPGTYSLFALSQEEIVARDFICFG
                     NPDAVIVVCDATCLERNLNLVFQVMELTDKVILCINLIDEARKKGITIDKKLLEDSLG
                     IPVILTAARNGSGMDELLDTLNDVSFDKYKLNNKPVRYNENIENVVKSIQPELDNIIP
                     GINSRWLGLRLIDGDESIFESMSNYIDKNSIDAINEVKKKIPDNINKQKIRDEFTKIN
                     YDYAKKLSDECCSNVAKKSTDREEKVDKILTSKIFGLPIMLLLLGTILWITIEGANYP
                     STLLSNLLLGFEPSISGILNSINCPSWLNDMLVLGLYRTLAWVISVMLPPMAIFFPLF
                     TLLEDFGYLPRVAFNLDHLFKKACAHGKQCLTMCMGFGCNAAGVIGCRIIDSPRERLI
                     AILTNNFVPCNGRFPTLIAISTIFFSSVITNSFVSSVATALCITLLIILGVIITLLVS
                     YTLSKTLLKGVPSTFTLELPPYRVPQIGRTLYTSIIDRTIFVLGRAVMVAIPAGVITW
                     IFANIYIGDLSILSHVANFLDPLAKLIGLDGFILLAFILGFPANEIVVPILLMAYLAT
                     GSMIELDSFSALGQVLREHGWTYLTALNVMLFSLLHWPCATTLLTIKKETGSLKWTAL
                     GFLMPTILAFVVCFLTTTVYNLFI"
     gene            complement(7273..8091)
                     /locus_tag="PC414_003149"
     CDS             complement(7273..8091)
                     /locus_tag="PC414_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2465526.1"
                     /translation="MEKQYFTTGEFAKLCGISKQTLIFYDKIGIFSPEYKDKNNYRYY
                     SVYQYDTLDILQSLREIGMSLEEIKEYIQNRTPQLCVKMLKEEEKKIREKIKKLRKIS
                     SKMQNRINITVEGISKMNSEEIYISKKFEEYLVLSSDLSNMSNSDFMMELIDFTNYCK
                     SKNLYQGYELGVIVSNENIKNGDYLSISSFYLKIDKKIKDKKLYVKPEGMYACIKHIG
                     KYEDSYKSYEKLKKYIYENEYKIVGNSYEESILDFFCESNEDNYMTEISIQVSR"
     gene            8298..8582
                     /locus_tag="PC414_003150"
     CDS             8298..8582
                     /locus_tag="PC414_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465527.1"
                     /translation="MLVRVKIVIGATIRYLIKHKVNYCNKKSLDKLELKYGNIDREKA
                     VKLEIFYQYLIELEYIEMSLLIRRLDTAITSLALVSIITIASYYLIRKNV"
     repeat_region   8750..8976
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=8817..8845
                     /rpt_unit_seq="atttacattccatatagttaatataaaac"
     gene            complement(9635..11728)
                     /locus_tag="PC414_003151"
     CDS             complement(9635..11728)
                     /locus_tag="PC414_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2465528.1"
                     /translation="MNKHNFEVILNQLQINIYVTNIHTNEIIFMNKKMKEEYNILDPE
                     GKVCWQVLYPEKNSTCSFCKVLELLKNDKKGVLIKWYEKCNKLNRVFENYDSLITWQD
                     GTVVHMHQSIDIANSTSLNKPIKINEFHEISNNKEEKGVFNFSRDNFDYNSTLLYDAL
                     IRGTDEYIYICNMKTGVFRYSPSQVELFDLPGEIVKNPLVYWKKIVHPEDWNRFYKSN
                     TEIGKNQMDYHTVEFRAKNRSGEYIWLRCRGQLMRDEFGEPSIFAGIMTQLGKQNKID
                     SLTQLLNYHEFMSVFEDKISNPMIEKLCIVLLDIDDFKNVNEMYDRDFGDNIIKTLAQ
                     SVQSILPDNAELYKLDGDEMGILVDNVEENEILTLYNQIQNMIIHLQLWRKYGLNITI
                     SAGCVIYPKHGDTVKELYKCASYSLQYAKEHGKNRLVFFSQEILKNKMYSLEMMRDLK
                     ASINDDFRGFSLRFQPQVDTESHKIIGVEVLLRWTNDKCKAISPLEFIPILEENDMIN
                     IVGAWVLRMALRTFRKWIDYYPFFKVSVNVSAVQILEDTFIEDIVKIIDDENFPYQNL
                     VLELTESHTVQNMSILQFKFKALQDLGIYIAMDDFGTGYSSLEVLKFSPIDIVKIDRV
                     FVKDILKSKFDATFIHFIVAICHDVGIKVCLEGVETQEEYDLVKQIKPDYIQGYLFGK
                     PQTATEIFDLLKLDN"
BASE COUNT         4211 a         1749 c         1420 g         4506 t
ORIGIN      
        1 gtaaacacct ttttaataaa tttttatatt ttttatattt ttttacctat actaaatttt
       61 tttatagata tttttaatat atatgatact ttaatattat gcaaataatg tcatataagt
      121 tacactttag ttatcttagt tacaactttg tcggtttact ttttctactt tcttcgatat
      181 aattataata tatttttgat acaattcgac aaaaggggga atgctgtaat tatggtaaaa
      241 aaaagaaaaa agaaaactgt tacacttact atacttgttg taatagttat tggtattttt
      301 tctatggcat gggcggacat gtctagaaaa aaagaaatga aagaaactca agaaagactt
      361 aaagctgaaa gattagaaaa agaaagaatt gaaaaagaaa aaaatgaacc tattattggt
      421 gcaaaaaaag aagctgtaaa agaatatgga tatgacgcaa aaatagtttg ggacaaactt
      481 aacaaatacg actattcaaa caatggagaa aagatagtct ttcttacatt tgatgatgga
      541 ccatctacta ctaatactcc acaagttcta gatatcttga aaagacatgg tgttagagga
      601 actttcttta ttaagggtga ttctcttgaa agaaaaggag ctaatgaaat tttaaaaaga
      661 acatttgacg aaggaaatgc aatagcccat cactcttata ctcatgacta taaaaaacta
      721 tatcctaaca gaagcctaaa tctcgataca ttcgtaaatg aacttaacaa aactgatgaa
      781 gctatgaaaa aagtcttagg aaagaatttt tcctcaaatg tagttagatg cccaggcggg
      841 tatatgtcat ggaaaaatat ggaacctcta ggaaactact taaaagagaa aaacatggca
      901 tcaattgatt ggaacgcttt aaatgctgat gcagaaggta aaaagaaaaa tgcacaagaa
      961 ttatttgaac atgcaaagaa atcttctgaa ggcaaagaaa tggttgttct tttaatgcat
     1021 gatacttatg gaaaacaaga aacagtaaat gcattggacc aaattataac atattttaaa
     1081 gacaatggtt atcaatttaa aattttaata taataaaaaa gcaagtttat tgaatgtaca
     1141 ttttatactc aatagacttg cttttacttt actctatttt actagctcga tttttacaaa
     1201 ttttttcttt cctatttgaa caactaattc actctctttt acaatagtgc ttaaatcctc
     1261 tactttttca ccatttacct ttactccacc ttgagaagct aatcttctaa cttcactctt
     1321 actcttaaca atctcattag aaactagtac ttcgattaag tcaaatccat cttccttaac
     1381 ttgaattgtt tgtatatctt caggtatttg acctttttgg aatacagatt taaatctttc
     1441 ttcagcttct ttagcagatt cttctccatg gtataatgtt actatttctc ttgctaagtt
     1501 catctttata tctcttggat ttactgaacc ctctttcaac tgtgattcta ttttattaac
     1561 ttcatcagga tgaacatctg tcactaaatt gtagtattta attataagtt catcaggaat
     1621 ttccatagat ttttggtaca ttattcccgc ttcttcatct attccaatat agttacctaa
     1681 actcttactc attttttctt taccatctaa tccttctatt aaaggcatca ttattactat
     1741 ttgagactcc ataccaaatt ctctttgtaa tgatcttccc attagtaaat tgaatctttg
     1801 gtcagttcca ccaagttcta tatcagcttc taatgctata gagtcatatg cttgcattaa
     1861 tgggtagaag aactcatgta ctgatattgg catttgtcct tcatatctct ttttaaaatc
     1921 ttctctttct aacattctcg caacagtaat agttgctgct aattttatta catcttcaaa
     1981 atttaactta gctaaccact cgctattaaa tcttacaata gttttttctt tatctaaaac
     2041 tttgaaaatt tgttcttcat aagtttttgc atttgcaaga acttgctctg ttgttaaagc
     2101 ttttctagct ttagattttc cagttgggtc tccaattttt cctgtaaaat ctccaattat
     2161 tattactatt tgatgaccta aatcttgtaa ttgtttcatc tttctaagaa ctactgtatg
     2221 ccctaaatgt atatctggag cacttgggtc tagacccaat tttactatca taggctttcc
     2281 ttctttttct gatttaataa gcttttccct taactctttc tcatctatta aatcatctac
     2341 acctttcata attattcgca tttgctcgtc tatcgatttc atttttcatt cctcctaaaa
     2401 tatttatata atctataatt tttattttta taataaaaaa agctttcatc ctcttaaaag
     2461 gacgaaagca ttgcttacgt gttaccacct tagttcatca atatctcacg atattaatct
     2521 cttagagtac aattacatat actcgtgtac tataacgtgt actaatccga tatctcttaa
     2581 tggttgttat ctttcagaga tagtgctctg agatgtattc aaactaaatc aatttattcc
     2641 atttcaccaa actggaactc tctgttaaat aaaatctagc cctacttctt ctcttcatag
     2701 catttatata ttcagattat ttcatcaatt acaagaaatt tttcttaact tactcataat
     2761 atattattat ttttatattt tgtcaacctt ttttattatt atatacatgt ttttttacat
     2821 tatactactt ttttatattt tttaccaata cccaaaattt atttttcaat caaaagattt
     2881 atttgtttat tgactatatt actctgactc aatcttatat ttattttctt atttaaaaat
     2941 gcgtttataa ttattaaacc aataactgtt acatcaaata taaaaaacag caataaaatt
     3001 attatcaaaa ataaaattct agcatataat aattttctaa acttagctat tataaagttt
     3061 tgtagtgaca tacatgagtc tctaatgata aaatagagac ttatataggt tattgatata
     3121 aagattgttt ttaaataaac agaatcaact ttaacattca acagttttag aacttcaaaa
     3181 atggctagat aagaaacaca tatgtaaaaa gctttttttg agcaacataa aaattggttt
     3241 aaacttctaa aattcttaaa tattaaaaat ttttttagag aattagaatc taaaatatgc
     3301 aatttattcc ctaaaattaa acttaataaa tatatgcaaa acaccatacc aaaaggaaac
     3361 aatgctatca ttaaataata aataaaattt gtatacccat taaaataaat ccatttgcta
     3421 tatccttgaa attctttcat aaaactataa cctattaatg ttgaagcata tatatcaata
     3481 aaaaccatta caataagccc aaatactgaa ccatatacta aatcatccat aaccattgta
     3541 ggcttattta ttagatagcc acacattata ccaagtaata cactctgact tgcccaaata
     3601 gcagttccta gatttccaag taccagagaa attattaaaa gcgaagtaat tccagataaa
     3661 atggtatact ttaaatcaca tttcaagcag attatacaaa aaaatactgg cactataaaa
     3721 tctaagtaaa tagcatatcc aaatccagta ctcacagcta taattgttac tactatgaat
     3781 aaagatgaca ataaagctgc ttcagttaat tttttagtag tattcaaaac actgcctcca
     3841 taaatcataa attttctaac taaagttttg tttttaatat agtatatcac tttttaaaaa
     3901 tatttttaat aaatataaat tttaataaag gtatttatgt taaaaatggt ataatgtatc
     3961 ttaatattca gttttttaag atgctaggag gtaattacta tgtttaaaat taaacacttt
     4021 aatgatttga gtttagatga attttatgaa atagcaaaat caagatatga ggtatttgct
     4081 tgtgaacaga agatattttc attaaacgat tatgatgata tagataaaaa ctcttatcac
     4141 atatttttaa aagaaaatgg attgatttgt gcttacgcta gaataattcc taaagaatac
     4201 agctcttata atgatgtttc cattggtaga gttttagttc tttctagtca tagaagaaaa
     4261 ggattagcaa aacaaatgat ggattgtgct atagatttta taaaagttaa cttacacgaa
     4321 aataatataa ccttatcagc acaaacttat ataaagaacc tttacttatc ttgtggtttt
     4381 aaagaaatct cagaagtata cgacgaagca ggtattgaac atattaaaat gagattatag
     4441 tgttgacaag ctaatattgt taatatataa tttaagtata aattaaatat tgaattgata
     4501 tttaggtatt tgaaaatacc ttacatagag tcagaaggct tttgctttct gactttttta
     4561 ttatcaaaat tcaataatta tatggattca aagtgttaat ttttattcta tattctactt
     4621 tccccaaaag taagaattat acccaacttt gtatgatagg catcttgcct atcttttttt
     4681 tgtaaaagta acaaggtaat aatataatgc atagtttaaa ataagaagat tagcccaggc
     4741 taaatttcaa gcatggaggt gctattatga aaaaccttaa cgatattcaa gtgaaaaaaa
     4801 ctgtcaaagt tgaagatata ttatctagtg gtaatttaag agaaagaatg ttagcgcttg
     4861 gtcttactag aggagctgtt atagatgtag ttagaaaagg tccaaaaaat aatttaactg
     4921 tctataaaat cagaggtagc aagatagctc tacgacaaga agaaagtagc ttaatattag
     4981 tttctgacat ttaaaaattt ctagcaggag gatattaata tggggttgac acataactca
     5041 actaaaatga gttctttaaa agacatgttt gatatagaca ataaggaaga ccagtttgta
     5101 attgcattgg ctggcaatcc aaatacaggt aaaagtactg tatttaatca tcttacagga
     5161 cttagacagc atactggaaa ctggccagga aaaactgtag ctacagctcg tggtaatttt
     5221 aaatataaaa ataccgaata tgcattaata gatttaccag gaacatattc tttatttgcc
     5281 ttatcacaag aagaaattgt agctcgtgat tttatatgtt ttggtaatcc agatgcagta
     5341 atagtagtat gtgatgctac atgtcttgaa agaaatttaa atttagtatt tcaagtgatg
     5401 gaacttacag ataaagttat tttgtgtatt aatttaatag atgaagctag aaaaaaaggc
     5461 attactattg ataaaaaact tttagaggat tctcttggta ttccagttat tttaactgct
     5521 gcaagaaatg gctctggtat ggatgaacta ttggatactt taaatgatgt atcttttgac
     5581 aaatataaat taaataataa acctgttaga tacaatgaaa atatagagaa tgtggtaaaa
     5641 tcaattcaac cagaattgga taatattata ccaggtataa attctagatg gttgggactt
     5701 agacttattg atggcgatga aagtatattt gaatctatga gtaattatat agacaaaaat
     5761 tctatagatg ctattaatga agtaaagaaa aagattccag acaatataaa taaacagaaa
     5821 ataagagatg agtttactaa aatcaactat gattatgcta aaaaactgag tgatgagtgt
     5881 tgttctaatg ttgctaaaaa atcaactgat agagaagaaa aagtagataa aatattaaca
     5941 tctaaaatat ttgggcttcc aataatgctc ttattattag gtactatatt gtggattact
     6001 atagaaggag ctaactatcc atccacttta ttatctaatt tattactcgg atttgagcca
     6061 agtatttcgg gtatattaaa tagtattaat tgtccatcat ggttaaatga tatgttggtt
     6121 ttaggattat acagaacatt agcatgggta atctctgtaa tgctacctcc tatggcaata
     6181 ttttttccat tatttaccct acttgaggat tttggatatt tgccaagagt tgcatttaac
     6241 ttagaccacc tatttaaaaa agcttgtgca catggaaaac agtgtttgac tatgtgtatg
     6301 ggatttggat gtaatgcagc tggagttatt ggttgtagga taattgactc tccaagggaa
     6361 agacttatag ctatacttac aaacaacttt gttccttgta atggtcgttt tcctactttg
     6421 atagctatat ctacaatatt ttttagctca gttataacca actctttcgt atcaagcgta
     6481 gcaactgcac tttgcataac tttactgata attctaggtg ttataataac attgttggtt
     6541 tcttacactc tttctaagac gctattaaaa ggagttccgt ctacatttac attagaactt
     6601 cctccttata gagttcctca aataggaaga actttatata cctctataat agatagaaca
     6661 atatttgttt taggaagggc tgtaatggta gccatacctg ctggtgtcat aacttggata
     6721 tttgcaaata tatatatagg agatttaagt atattatcac atgttgcaaa ctttttagac
     6781 cctttagcta aattaattgg tcttgatggt tttatattac ttgcttttat tcttggattc
     6841 ccagcaaatg agatagttgt acctatactt ttaatggctt acctagcaac tggttctatg
     6901 atagaattag atagctttag tgctttaggt caagttctta gagaacatgg atggacttat
     6961 ttgacagctt taaatgttat gctgttcagt ttactgcatt ggccttgtgc aacaacactt
     7021 ttaacaataa aaaaagaaac tggtagttta aaatggactg ccttaggatt cttaatgcct
     7081 actatacttg cttttgttgt ttgtttttta acaactacag tttacaattt atttatataa
     7141 ttagtattaa ctgacagtag acatatatta gagaaaccat ttaaaaattt atatacttta
     7201 ttataaagca tagagatata ttcaatgata agtaaaatta cttaacattt gaatatatct
     7261 ctattttaat tcttatctgg atacttggat tgaaatttca gtcatataat tatcttcatt
     7321 actttcacaa aaaaagtcaa gtatactctc ctcatacgaa tttccaacaa ttttatattc
     7381 attttcatat atatattttt tcaatttttc gtaagattta tatgaatcct catattttcc
     7441 aatatgtttt atgcaagcat acattccttc tggctttaca tatagctttt tatcctttat
     7501 ttttttatct atcttcaaat aaaaacttga tatacttaaa tagtccccat tttttatatt
     7561 ttcattactt actattactc ccaactcata tccttgatac aaatttttcg atttacaata
     7621 attggtaaaa tctattaatt ccatcataaa atcactatta ctcatattac tcaaatcgct
     7681 tgatagaact aaatattctt caaacttttt ggatatataa atttcttcac tattcatttt
     7741 agaaattcct tcaactgtta tatttattct attttgcatc ttagaactta tctttcttag
     7801 tttttttatt ttttctctta tctttttttc ttcttcttta agcattttca cacaaagctg
     7861 gggagttcta ttttgtatat actcttttat ttcctctaaa ctcattccta tttctcttaa
     7921 tgattgtaat atatcaagtg tatcatattg ataaactgag taatatctat aattattttt
     7981 atctttatat tcaggagaaa aaatacctat tttgtcatag aaaattaatg tctgcttaga
     8041 tataccacac aatttagcaa attcacctgt tgtaaaatac tgtttttcca agggttatac
     8101 actccttttc tttttgttgt ctatatagtt actatattct ttattattta ctaaatcaag
     8161 tagttattat tttagaaaat caatatggtt ttgattttaa agcttaaata tcagttagat
     8221 gtcagttata aatattttac cctttcccat cataaaaaag atatcccctc ttaacttttg
     8281 gatggaatat tatatttata cttgttagag tgaaaattgt aataggtgct acaataagat
     8341 atttaattaa acataaagtt aattattgta ataaaaaatc tttagataaa ttagagttaa
     8401 agtacggaaa tatagataga gaaaaagctg ttaaactaga aatattttat cagtatttaa
     8461 tagaacttga atatatagaa atgagtttat taataagaag attagatact gcaataacat
     8521 cattagcatt agtatctatt ataacaatag catcatatta tttaatcaga aaaaatgtgt
     8581 aattatgtaa gctatttata ataaaacact gataaaatta ataataaaaa ggtaatagga
     8641 tttctatact acctttttgt ttttataata tagtgtaatt ttattatatt tttgtatgct
     8701 aatgtaacta gataatcata agataacaca ttaaaagaaa attatgaaaa cttacattct
     8761 gtatagttaa tataaaacat aagtaatata aatttatgta aagcaacaaa agatagattt
     8821 acattccata tagttaatat aaaaccttag agaaagcgaa aacgtaccat taaaatcaaa
     8881 tatttacatt ccatatagtt aatataaaac gttgtaatca tagttccaat tagccacttt
     8941 aacatgcatt tacattccat atagttaata taaaacacca aaataaactt agtattttca
     9001 acacttacac gtatacaact ctcctaaatt tgcagtgaac gagcaatagt gcaattgata
     9061 acgcttatca tgcactctca acgccttata tttcaactgt tacgccaaat tttttcacaa
     9121 atatcgctca ctgcaaaatt cctataattt tattatatca taaatatatt acttttgaat
     9181 attgatacca atttatacca taataaaagc aaaaaaacgt aatctatcta cattagattt
     9241 aaattctata acttaaagtt ttttacataa tatttatgct tgtttaatca atctaatata
     9301 tcattcttgt atattgcttt taatagtctt ttttcttaaa taaatatcta tctgaacaag
     9361 catagtttaa ttaaaaactt ttgtattccc ttaaaactgt atctacctct tattatttta
     9421 tttatgatat attattttta atctaaaaat agatatctat ttttattatg gttatccctt
     9481 tttaaaatac tttaatcata tactattccc aatttaacat ccattgaaat gctattttct
     9541 tatattgcaa tttataattg atatatcttc aatataataa tttaatgtat ttttgatttt
     9601 aaagcttttc agtgatatta ttgataaatt agaattaatt atctagcttt aaaaggtcaa
     9661 agatttctgt tgctgtctgc ggttttccaa ataaatatcc ttgtatgtaa tctggcttta
     9721 tttgtttaac taaatcatac tcttcttgtg tttctacacc ttctaaacat acttttattc
     9781 ctacatcatg acaaattgca acaataaaat gtataaatgt agcatcaaac ttactcttta
     9841 atatatcttt aacaaatact ctatcaattt tcactatatc tattggagaa aattttaaaa
     9901 cttctagaga cgagtatcca gtaccaaaat catccatagc aatataaatt cctaaatctt
     9961 gtaaagcctt aaatttaaac tgtaaaatac tcatattttg tactgtatga ctttctgtca
    10021 attctaaaac aagattttga taaggaaaat tttcatcatc tataattttt actatatctt
    10081 caataaatgt gtcttctaat atttgaacag ctgaaacgtt tacactcact ttaaaaaaag
    10141 gatagtaatc aatccatttc ctaaaagtac gtaatgccat tcgaagcacc caagccccta
    10201 caatatttat catatcattt tcttctaata ttggaataaa ttccaaagga gaaattgctt
    10261 tgcatttgtc attagtccac cttaaaagaa cttctacacc tattatttta tgactttctg
    10321 tatctacttg tggctggaat cgaagactaa accccctaaa atcatcatta attgatgcct
    10381 ttaaatcccg catcatttct aatgaataca ttttattttt taatatttct tgggagaaaa
    10441 aaacaagcct gtttttacca tgctctttcg catattgtaa agagtaacta gcacacttat
    10501 aaagttcttt tactgtatct ccatgttttg gatatattac acaaccagca gaaattgtta
    10561 tatttaatcc atattttctc catagttgaa ggtgtataat catattctgt atttgattat
    10621 atagagttag aatttcattt tcttcaacat tatcaacaag aatgcccatt tcgtccccat
    10681 caagtttata aagttcagca ttatctggta aaatagattg tacagactgt gctagtgttt
    10741 ttattatatt atctccaaaa tccctatcat acatctcatt aacattttta aaatcgtcaa
    10801 tatctaataa tactatgcat aatttttcaa tcataggatt tgaaatttta tcttcaaata
    10861 cactcataaa ttcatgataa ttcagtaact gtgtcaatga atcaatctta ttttgtttac
    10921 ctaattgcgt cattattcct gcaaaaatac ttggctctcc aaattcatca cgcattagtt
    10981 gacctctaca tcttaaccat atatattctc cactacgatt tttagcccta aattccactg
    11041 tatgataatc catctggttt ttacctattt cagtgtttga cttataaaaa cgattccagt
    11101 cttctggatg tacaattttt ttccagtaaa caagcggatt tttaacaatt tctcctggta
    11161 agtcaaataa ttcaacttgt gatggtgaat atcgaaatac gcctgttttc atattgcaaa
    11221 tgtatatata ttcatctgta cctctaatca aagcatcgta aagcaatgta gaattataat
    11281 caaaattgtc tcttgaaaaa ttaaaaactc ctttttcttc tttattattt gatatttcat
    11341 gaaattcatt aatcttaatt ggtttattta agcttgttga gtttgcaata tctattgatt
    11401 gatgcatatg aacaactgtg ccatcttgcc atgtaattaa actatcataa ttttcaaaaa
    11461 cacgatttaa cttattacat ttttcatacc actttattaa tactcctttt ttatcattct
    11521 ttaataattc taaaacttta caaaaactac aagtagaatt tttttcagga tataatactt
    11581 gccaacaaac ttttccttct ggatctaaaa tattatattc ttctttcatt tttttgttca
    11641 taaaaataat ttcattagta tgaatatttg taacatatat attaatctgt agttgattta
    11701 atataacttc aaaattatgt ttgttcatat acttatctcc tccatagtta ctgctcttta
    11761 taacaacatt gttaaaatta ttctttttag aagatttact tataacttat acatatacac
    11821 taaatacctt aatttaatta gaaagtttat tacttcatat tttaaattat aattgataaa
    11881 aaaaaa
//
LOCUS       DAMJVK010000108        11873 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.113, whole genome shotgun sequence.
ACCESSION   DAMJVK010000108 DAMJVK010000000
VERSION     DAMJVK010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11873
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.113"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            227..691
                     /locus_tag="PC414_003152"
     CDS             227..691
                     /locus_tag="PC414_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HDF2465529.1"
                     /translation="MATMTDIIEKFIKDLMEEDNSIQIQRNELANLFSCAPSQINYVL
                     TTRFTIDRGYYIESKKGGGGYVQIEKIRKSKDGHIRELLNEKIGSQISYKKAKELLEG
                     LKESDLINERELKLILYAIDDKSLCMPIYELKEKVRSNILKNIIIGLFSIEE"
     gene            698..1192
                     /locus_tag="PC414_003153"
     CDS             698..1192
                     /locus_tag="PC414_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HDF2465530.1"
                     /translation="MLCQKCNKNKASVYYNKIVNGEKTEMYLCSECAKENTEMNFNLD
                     MPFSMMDIFSNLGFQPKKELEEKLVCPKCNTTYSEFKNNGRFGCSECYNAFSSQVNPM
                     LQNIHGHIEHTGKAPKKSFYKISVENEIKELKEDLDRAIKNEEYELAAQFRDKIKYLK
                     GSID"
     gene            1208..2233
                     /locus_tag="PC414_003154"
     CDS             1208..2233
                     /locus_tag="PC414_003154"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HDF2465531.1"
                     /translation="MKENIVMKSRVRLARNLNNYPFPNKLDKECAMEIIEKVKNAFIN
                     SNLEQKEEFDFYKIEDLDQSKKMLMVEEHIISPDLAENDKSAVIVKKDKTISIMINEE
                     DHIRIQTICDDLNLEYAYSVANEIDDLLESSLEYAFNTKLGYLTSCPTNTGTGMRASV
                     MMHLPALSQLGYMDELYKISSQIGIAIRGIYGERTEALGNIYQISNQLTLGRTESNII
                     ENVSGLTKDAISKEIKAREILQKKLGIKLEDKIFRSIGTLENSRVMSSAEAMSHLSNI
                     KMGIEMNYIDKLDLRAIEQLMIGIQPAHQSIMYKSDDVENRDINRATYIRETLEKLRG
                     GMNCELQ"
     gene            2220..4667
                     /locus_tag="PC414_003155"
     CDS             2220..4667
                     /locus_tag="PC414_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HDF2465532.1"
                     /translation="MNFNRFTQRAKKAIDLAFESAKSLGHNIVGSEHILLGLLREEEG
                     IAAKVLSKVGFTEAYLEGKIVDMEGKGEEISEDIVLSPRSKQILELSGMFANKLKTNY
                     IGTEHILLAIIQEGEGIANKILNYAGVNDRTLAQLTIDMMGISDKNQYKAENSYTGNQ
                     NQAESKVLDKYGRNLTLYAKQNKIDPVIGREKEIQRVIQILSRRTKNNPVLIGDPGVG
                     KTAIAEGLATNIALGNVPETLKNKTLYSLEMGSLLAGAKYRGEFEERIKEVVDEVVKN
                     GNIILFIDEMHTIIGAGSTGEGSIDASNILKPALARGEIQVIGATTIDEYRKHVEKDS
                     ALERRFQPVMVDEPTKEDSIKILEGLRDKYEAHHKVKITDDAIKAAVELSTRYISDRY
                     LPDKAIDLIDEAASKVRLKENTPPSEIKKLELEIENIDKEKEEAVRCQDFEKAAKIRD
                     EQGLLKKQLEDVRERWNKSSKHSDLVDGEVIAEVVGLWTGIPVNKILEEEADRLLRLE
                     EILHNKVIGQEQAVKSISKAIRRSRAGLKDPNRPIGSFLFLGPTGVGKTELSKALAEV
                     QFGDENQIIRIDMSEYMEKHAVSRMIGSPPGYVGHDEGGQLTEKVRRNPYSVILFDEI
                     EKAHPDVFNILLQILDDGRLTDSKGRTVDFKNTIVIMTSNVGASTIGRQKTLGFSIAK
                     GDEEEKSQYEKMKENIMGELKQRFRPEFLNRIDDIIVFHSLNENHISKIVLLMAAKLQ
                     ERLKEMDIKLEMSDEAVKLISKSGFDLEYGARPLKRALQKELEDELSEAILKGNVKKG
                     SNVVAKVKDEKIVFETK"
     gene            4847..6220
                     /gene="radA"
                     /locus_tag="PC414_003156"
     CDS             4847..6220
                     /gene="radA"
                     /locus_tag="PC414_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDF2465533.1"
                     /translation="MAKIKTKYVCQSCGYETAKWLGKCPECTKWNTFVEEIDQKSTKK
                     EVFIIDKSSSKPVSINSIESKEEERFTTDINELDRVLGGGIVKGSLVLVGGDPGIGKS
                     TLLIQVSSNVANLGKTVLYITGEESESQIKMRAKRLGINSEKLYIFAENNLSIIESYL
                     ESVNPELIILDSIQTVFSPEISSAPGTVSQIKEGTSKFMKISKKMGISTFIVGHVTKE
                     GSLAGPKLLEHMVDTVLYFEGERYNTYRLVRAVKNRFGSTNELGVFEMRDLGLVELDN
                     PSKILISEKPKDVAGSVIISTVEGTRPMLLELQALVSPTSFGIPKRTSTGVDYNRVGM
                     LLAVLEKRVGLQIQNQDVYINIVGGIKINEPSIDLGIAISVASSFRNIPIDEDIAVTG
                     EVGLTGEVRAVSFIEKRIAECKKLGFKKIVVPRSNYDVVKETKGIEIWPVDNLRQAIN
                     IVLGRNQ"
     gene            6223..7293
                     /gene="disA"
                     /locus_tag="PC414_003157"
     CDS             6223..7293
                     /gene="disA"
                     /locus_tag="PC414_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning protein DisA"
                     /protein_id="HDF2465534.1"
                     /translation="MENFLDNKNMLYALKMISPGTPLRLGLNNVLRAKTGGLIVIATN
                     EDVMKIVDGGFAINAEYSPSYLYELAKMDGAIVLSGDVKKILFANAQLIPDYFIETSE
                     TGTRHRTAERVAKQTGAIVIGISQRRNVITVYRGNEKYVVEDISKIFTKANQAIQTLE
                     KYKTVLDQAVTNLNALEFNDLVTIYDVALVMQKMEMVMRVTSIIEKYVIELGDEGTLV
                     SMQLEELMGTTRIDQKLIFKDYNKENTEIKELMKKVKNLNSEELIELVNMAKLLGYSG
                     FSESMDMPIKTRGYRILSKIHRLPTAIIENLVNYFENFQQILDASIEELDEVEGIGEI
                     RATYIKNGLIKMKQLVLLDRHI"
     gene            7382..8476
                     /locus_tag="PC414_003158"
     CDS             7382..8476
                     /locus_tag="PC414_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HDF2465535.1"
                     /translation="MIRKVTRILFILLGFTIGITTYLTLMKDFEILTFGKETYGYIAA
                     VVAGIIIAILGYLIEPWVVNKVKEIAKIVDKELSKYPQTDILLGSMGLIVGFVIAYLL
                     SGLVNRIPIVGGILSLLLYLFLGYLGMKVALKSKNDLFNIGKLGRLANPIKDKDKDKE
                     NKKEVKAIPPKVLDTSVIIDGRIADICKTGFIEGKLIIPAFVLEELRHIADSSDDLKR
                     VRGRRGLDILNIIQKELNIEVEISERDFDDIAEVDSKLLKLAQVLNGKVVTNDYNLNK
                     VAQFQGVEVLNINELANAIKPVAIPGEDMVVQVVKEGKEAQQGVAYLDDGTMIVVDGG
                     RKYMNETIKVLVTSVLQTPAGRMIFAKPKN"
     gene            8605..10782
                     /locus_tag="PC414_003159"
     CDS             8605..10782
                     /locus_tag="PC414_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465536.1"
                     /translation="MRKKIISFLATFIIILTSASQYSFADDISTRGKVIFIDMNRTSM
                     SNMLRIKSLREELDNRGYIGLMNIRGDKGSDDRRSYASMGAGGRANVANEEDINFESS
                     SKDRNIVFESATGKSAKGINNLTINKSINENLNFGEYGSVLGSLGQSLSDNGLKASVL
                     GNSDIIENGQLIKNRNLCLTAMDEYGRIPNGNVDTINKKDLSMPYGISTDYDKLIVET
                     KEAYKNNDVIFVELGDTYRLDLYKPNLNEKTYESMKDNIEKNIDIYLKNIFSMVEEND
                     TVYIASAFPSDLDYKNKRRLSPIIKLNGEGKGILSSSTTRREGIVTNLDVGVEILDNF
                     NIKNQNMVGRKYELINRDDNKEFLMDEYQKIVSISSIRSTILNGFVSIVFLSWIVAMI
                     AILFRKHISKNYKETTFFILKELLKIGIVMPLSFMITPIMNFKTPLAISLGIIIITLT
                     IYLISKVLIKNDLKNMLFFTGLTIVIMVIDAGFGSYLMKSNVMSYDCIIGARYYGVGN
                     EYQGVAIGSAIFTFAILLTYKNIPKWSIIVFSLVILITSASPIMGANVGSAISECVAF
                     LLFILLIYNVKIDFKKIVLLGIAVVFVLGVFVAIDMILGSNSHLGMFVKEIYFNGPGE
                     IIQTFSRKIEMNLKLAQTSAWVNILLTGIGVILVLMINQIRYFKQLMDEYPIVFKGFI
                     ASIAGCLVTLLVNDSGIVSSATAFIYVIVPMITLSINLTALKE"
     gene            complement(10962..11813)
                     /locus_tag="PC414_003160"
     CDS             complement(10962..11813)
                     /locus_tag="PC414_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2465537.1"
                     /translation="MNRNNFSHEIIEPIDNLDVNFKLFDDSGSYVANHWHNSLEIIYI
                     TSGDLQINMEGYTYNLKANECMFINSGIIHSTRCIYQNTSILLQVPLSFLNKYIPDFK
                     NCYFDFKVNANDNNYKKNSSKVKLILENMREIKLSSPPAANLLFTSLLFELLFELYTN
                     FKISVGNKNMKKTVLDLSKFEPVLEYTKINYKMPISINKIAKVAHLQPEYFCRKFKQY
                     MGQTYLEYLNDVRISHIYKDLINTNDTLCSILEAHGFTNTKLFYRIFKEKFKCTPKHI
                     RKNLPKI"
BASE COUNT         4920 a         1212 c         2125 g         3616 t
ORIGIN      
        1 tataaagtat ttaaagttct gaaatctaat catgaagcaa aaaattagca tttggttatc
       61 aataaatata ataaataaaa actcataatt aatgaaagaa taaattcata tctaaatttc
      121 tataaaaaat ttaaatttat acaatttagg gctttaaaaa tttaaataat aggtgtaaaa
      181 taatagtcaa agaaagtcaa aaataaaaag gtgtgtggtg atattaatgg caactatgac
      241 agatataatt gaaaaattta ttaaagattt gatggaagaa gataatagca tacaaattca
      301 aagaaatgaa ctagcaaact tgtttagttg tgcaccatca caaataaatt atgtcttaac
      361 aacaagattt acaatagata gaggatatta cattgaaagt aaaaaaggtg gcggaggata
      421 tgttcaaata gaaaaaatac gaaaaagtaa agatgggcat attagagaac tactaaatga
      481 aaaaatagga agtcagatat cctataaaaa agcaaaagaa ttattagaag gtttaaaaga
      541 gtcggatttg attaacgaaa gagaattaaa attaatatta tatgcaatag atgataaatc
      601 attgtgtatg ccaatatatg aattaaaaga gaaggtaagg tctaatattt taaagaatat
      661 aataatagga ttatttagta tagaggagtg ataatttatg ctatgtcaaa aatgcaataa
      721 gaataaagca tctgtatatt ataataagat agtcaatgga gaaaaaacag agatgtattt
      781 atgtagtgag tgtgcaaaag agaatacaga aatgaatttt aacttagata tgcctttttc
      841 aatgatggat atattttcta atttaggttt tcaacctaaa aaagaacttg aggaaaagct
      901 agtttgtcct aaatgcaata caacatatag tgagtttaag aataatggga gatttggatg
      961 tagtgaatgt tataatgcat ttagtagtca agtaaatcca atgcttcaaa atatacatgg
     1021 gcatatagaa catacaggaa aagcacctaa aaaatctttt tataagataa gtgtagaaaa
     1081 tgagattaaa gaactaaaag aagatttaga tagagctata aaaaatgaag agtatgagtt
     1141 agcagctcaa tttagagata aaattaaata tttaaaaggt tctattgact aagattggag
     1201 tgatattatg aaagagaaca tagtcatgaa atcaagagtt aggctagcta gaaatttaaa
     1261 taactatcca tttccaaata aattagataa agaatgtgcc atggaaataa tagaaaaagt
     1321 aaaaaatgcg tttattaatt ctaacttaga acaaaaagaa gaatttgatt tctataaaat
     1381 tgaagattta gaccaatcta aaaaaatgct tatggttgaa gaacatataa taagcccaga
     1441 tttagcagaa aatgataaaa gtgcagttat agtaaaaaaa gataaaacta taagtataat
     1501 gataaatgaa gaagaccata taaggataca aactatatgt gatgatttaa acttagaata
     1561 tgcatatagt gtagcaaatg aaatagatga tttgttagaa tcaagtttag aatatgcctt
     1621 taacacaaag ctaggttatc taacatcttg tccaactaat acaggaacag gaatgagagc
     1681 ttctgtaatg atgcatttac cagcattaag tcaactaggc tatatggatg aactttataa
     1741 aatttcatca caaataggta tagcaattag agggatatat ggagaacgaa cagaagcgtt
     1801 gggaaatata tatcaaatat ctaatcaact tacacttgga agaacagagt ctaatattat
     1861 agaaaatgta agtggactta caaaagatgc aatttcaaaa gaaataaaag ctagagaaat
     1921 attgcaaaaa aaattaggta taaaactaga agataaaata tttagatcga tagggacact
     1981 tgaaaattca agagtaatga gtagcgcaga ggcaatgagc catctatcaa atataaaaat
     2041 gggaatagaa atgaactata tagataaact agatttaagg gctatagaac aacttatgat
     2101 aggtatacaa ccagcgcatc agtcaataat gtataagagt gatgatgttg agaatagaga
     2161 tataaataga gcaacatata ttagagaaac tttggaaaaa ttaagaggag ggatgaattg
     2221 tgaacttcaa tagatttaca caaagagcta aaaaggcaat tgatttagcg tttgaatctg
     2281 ctaaaagtct aggacacaat attgtcggaa gtgaacatat acttttaggt cttttaagag
     2341 aagaagaagg tatagctgct aaagttctaa gtaaagttgg atttacagaa gcttatttag
     2401 aaggcaaaat agttgatatg gaaggtaagg gagaagaaat ctcagaagat atagtattaa
     2461 gtccaaggag taaacaaata cttgaattat caggaatgtt tgcaaataaa ttaaaaacaa
     2521 attatattgg gactgagcat atattattag caattattca agaaggtgaa ggaatagcta
     2581 ataagatttt aaattatgct ggagtaaatg ataggacttt agcccaatta acaattgata
     2641 tgatgggtat tagtgataaa aatcaataca aagcagagaa tagttacacg ggcaaccaga
     2701 atcaagcaga atctaaagta cttgataagt atggaagaaa tcttacacta tatgcaaaac
     2761 agaataaaat agaccctgta ataggtagag aaaaagaaat acaaagagta atacaaatat
     2821 taagtagaag aactaaaaac aatccagtgt tgataggaga cccaggtgta gggaaaacag
     2881 ccatagctga aggtctggct acaaatatag ccttaggaaa tgttccagaa acactaaaaa
     2941 acaaaacttt atactcttta gagatgggtt cattattggc tggagctaag tatagaggag
     3001 aatttgaaga aagaattaaa gaagttgtag atgaagttgt taaaaatgga aacataatct
     3061 tatttataga tgagatgcac acaataatag gggctgggtc tactggagaa ggctctatag
     3121 atgcttcaaa tatattaaag ccagccttag caagaggaga aatacaagtt ataggtgcaa
     3181 ctacaattga tgaatataga aagcatgttg aaaaagattc tgctctagaa agaagattcc
     3241 aaccagttat ggtagatgaa ccaactaaag aggactcaat aaaaatatta gaaggattaa
     3301 gagataaata tgaagctcat cataaagtta aaataactga tgatgctata aaggcagctg
     3361 tagaattatc aactagatat atatcagaca gatatctgcc agataaagct atagacttga
     3421 ttgatgaagc tgcatcaaaa gtaagattaa aagaaaatac acctccttca gaaataaaaa
     3481 aattagaact agaaatagaa aatatagata aagaaaaaga agaagcagta agatgtcaag
     3541 actttgagaa agcagccaaa ataagagatg aacaaggtct acttaaaaaa caattagaag
     3601 atgttagaga aagatggaac aagtcatcta aacattcaga tttagttgat ggagaagtta
     3661 ttgctgaagt agtgggctta tggacaggaa taccagttaa taaaattctt gaagaagagg
     3721 ctgatagact tttgagactt gaggaaatac tgcacaataa agttataggt caagaacaag
     3781 cagtaaaatc tatttcaaag gcaatcagaa ggtcaagagc aggtcttaaa gaccctaata
     3841 gaccaattgg ttcattttta tttttaggtc caacaggagt aggtaaaaca gaattatcta
     3901 aggcattagc agaagtgcaa tttggagatg aaaatcaaat aattagaata gatatgtctg
     3961 aatatatgga aaaacatgct gtgtcaagaa tgataggttc acctccaggt tatgtaggtc
     4021 atgatgaagg tggtcaatta actgaaaaag taagaagaaa tccatattca gttatcttat
     4081 ttgatgagat agaaaaagct catccagatg tatttaatat tctgttacaa attctagatg
     4141 atggtagact aactgattca aaaggaagaa ctgtagattt taagaatact attgtaataa
     4201 tgacatcaaa tgttggagca tctacaattg gtagacaaaa aactttagga tttagtatag
     4261 ctaaaggaga cgaagaagaa aaatctcaat atgaaaaaat gaaagaaaat ataatgggtg
     4321 aattaaaaca aagattcaga ccagagtttt taaacagaat agatgatata attgttttcc
     4381 actcactaaa tgaaaatcat atatcaaaaa tagtactttt aatggcagcg aaactacaag
     4441 agagattaaa agaaatggat ataaaattag aaatgagtga tgaggctgtt aaattaattt
     4501 ctaaatctgg atttgactta gaatatggag caagacctct taaaagagct ttacaaaaag
     4561 agttggaaga tgagctatct gaagcaatct taaaaggtaa tgtgaaaaag ggaagtaatg
     4621 tagtagcaaa agtcaaagat gaaaaaatag tttttgaaac taaataattt aatagagttg
     4681 cattttaaaa tattgcaaat ataagtttta taatctaaac aaaattttaa gggcaatgaa
     4741 tctataataa ttcattgtcc ttaattattt tatttaatct ttacatattg tctttcagat
     4801 atgctaaaat tatagtatag aataaaaaat taaggatgat aaaagtatgg caaaaataaa
     4861 aacaaaatat gtatgtcaat cttgcggata tgaaacagct aaatggcttg gtaaatgtcc
     4921 agaatgtaca aaatggaata catttgtaga agaaatagac caaaaaagca ccaaaaaaga
     4981 ggtctttata atagataaat cttcatctaa accagtcagt ataaactcaa tagagagtaa
     5041 agaagaagag cggtttacaa cagatataaa tgaattagat agagtgcttg gaggaggtat
     5101 agttaaaggc tctttagtgc ttgttggtgg tgaccctggt ataggtaaat caacactttt
     5161 aatccaagta tctagtaatg ttgccaattt aggaaaaact gttttatata taactggaga
     5221 agaatcagaa tctcaaataa aaatgagagc taaaagatta ggtattaact cagaaaaatt
     5281 atatatattt gctgaaaata atttaagtat aattgaatct tatttagaaa gtgtaaatcc
     5341 agaattaata attctagact caattcaaac tgtcttcagt ccagaaatat cctcggcacc
     5401 aggaactgtc agccaaatta aagaaggtac ttcaaaattc atgaaaattt ctaaaaaaat
     5461 gggaatttca acgttcatag ttgggcatgt tacaaaagaa ggttcactag caggaccaaa
     5521 attattagag catatggttg atactgttct atattttgaa ggagaaagat ataatactta
     5581 tagattagta agagctgtta aaaatcgatt tggttcaact aatgaacttg gagtatttga
     5641 aatgagggat ttaggattag tagaacttga taatccatct aaaattttaa tctctgaaaa
     5701 accaaaagat gtagcaggtt cagtaataat atctactgta gaaggtacga ggccaatgtt
     5761 acttgaattg caagcattag tttctcctac aagttttggt ataccaaaaa gaacatctac
     5821 aggagtcgat tacaatagag taggtatgtt attagcagta ttagaaaaac gtgttggttt
     5881 gcaaatacaa aatcaagatg tctatataaa catagttgga ggaataaaga taaatgaacc
     5941 ctcaattgat ttagggatag ccatttctgt agcatctagt tttagaaata taccgataga
     6001 tgaagatata gctgttacag gagaagtggg acttacagga gaagttagag cagtaagctt
     6061 tatagaaaag agaatagctg agtgtaaaaa acttggattt aaaaagatag tagttcctag
     6121 aagtaattat gatgttgtaa aagagaccaa aggcatagaa atatggccag tagataactt
     6181 aagacaagct ataaatatag tacttgggag gaatcaataa atatggagaa ttttctagat
     6241 aataaaaata tgctatatgc attaaaaatg atatctcctg gaactccact tagattaggt
     6301 ctaaacaatg tactaagagc taagactggt ggattaattg taattgcaac aaacgaagat
     6361 gtaatgaaaa tagtagatgg aggatttgct ataaatgcag aatattcacc atcatatcta
     6421 tatgaattag ctaaaatgga tggagctata gttttaagtg gtgatgtaaa gaaaatatta
     6481 tttgctaatg cacaacttat acctgactat tttatagaaa catcagagac aggaacaaga
     6541 catagaacag cagaaagagt agcaaaacaa actggtgcta tagtcatagg aatttcacaa
     6601 agaagaaatg ttataacagt ttatagagga aatgagaagt atgtagtcga agatatatct
     6661 aagatattta ctaaggcaaa tcaggctata caaactctgg aaaaatataa gacagtattg
     6721 gaccaagctg taacaaattt aaatgcctta gagtttaatg atttggtaac tatttatgat
     6781 gttgcattag tcatgcaaaa gatggaaatg gtaatgagag ttacaagtat aattgaaaaa
     6841 tatgtgatag aattgggtga tgaaggaact ttagtaagta tgcaattaga agaattaatg
     6901 ggtacaacca gaatagacca gaaattaata ttcaaagatt ataataaaga aaacacagaa
     6961 ataaaagaac ttatgaaaaa ggtcaaaaat ttaaattcag aagaactaat agaattggtc
     7021 aatatggcaa aactattagg gtatagtggt ttttcagaaa gtatggatat gcctataaaa
     7081 acaagaggtt ataggattct tagcaaaata catagactac caacagcaat aatagaaaac
     7141 ttagtaaatt attttgaaaa ctttcaacaa attttagatg catctattga agaattagat
     7201 gaggttgaag gaataggtga aataagagca acatatataa aaaatggact cataaaaatg
     7261 aaacaattag tcttattaga tagacacata tgatgaattt taaatgaatt ttactacttt
     7321 tttcttgaat ataatataca ctttggtcta taatagataa gtataactag ggggtgaagc
     7381 attgataagg aaagtaacaa gaatattgtt tattttactt ggatttacaa taggtataac
     7441 aacctattta acattaatga aagattttga aatattaaca tttggaaaag aaacatatgg
     7501 ttatatcgca gctgtagtag caggtataat aatagccatt ttaggatatc ttatagagcc
     7561 atgggttgta aataaagtaa aagaaattgc taaaatagtg gataaagaat tgtcaaaata
     7621 tcctcagact gatatattgt tagggtctat gggtcttata gtaggatttg ttattgcgta
     7681 tttattaagt ggattagtaa atagaatacc aatagtaggt ggaatactat ccttactatt
     7741 atatttattc ctaggatatc ttggaatgaa agttgcgctt aaaagtaaaa atgatttatt
     7801 taatattgga aaactaggaa gattagcaaa tcctattaag gataaagata aggataaaga
     7861 aaataaaaaa gaagtaaaag ccattcctcc aaaagtatta gatacaagtg taataattga
     7921 tggaagaata gctgatattt gtaagactgg gtttatagaa ggaaaactta ttattcctgc
     7981 ttttgttttg gaggaactaa gacatattgc agattcttca gatgatttaa aaagagtaag
     8041 aggtagaaga ggtctggata tattaaatat aattcagaaa gaattaaata tagaagttga
     8101 aataagtgag agagactttg atgatatagc agaagttgat agtaaattat taaagcttgc
     8161 tcaggtttta aatggaaaag ttgttacaaa tgattataac ttaaataaag ttgctcaatt
     8221 ccaaggtgtt gaagtattaa atataaatga gttagctaat gctataaaac cagttgctat
     8281 accaggtgaa gatatggttg ttcaagtggt taaagaagga aaagaggctc aacaaggtgt
     8341 tgcatatcta gatgatggaa ctatgatagt tgttgatgga ggaagaaaat atatgaatga
     8401 gactataaaa gtactcgtta cttctgttct tcaaactcct gcaggtagaa tgatatttgc
     8461 aaaacctaaa aattaatatg ttcaaagaaa cctataattt gggctgttta ttttaaatag
     8521 atggtccaaa tttatttatt tataacgata atgtttaatg tattcttaaa gatggggatt
     8581 taaatcagaa agcagggaga atttatgaga aaaaagatta tatctttctt agcaacattt
     8641 atcataatat taacatcagc aagtcagtat agttttgcag atgatatatc aacacgtgga
     8701 aaagtaatat ttatagatat gaatagaact agtatgagta atatgttaag aataaaatca
     8761 ttaagagaag aactagacaa tagagggtat attggcttga tgaatataag aggcgataaa
     8821 ggaagtgatg atagaaggtc atatgctagt atgggtgctg gtggtagagc taatgtggct
     8881 aatgaagaag atattaattt tgaaagctcg agtaaggata gaaatatagt ttttgaatca
     8941 gctacaggaa aaagtgcaaa aggtataaat aatttaacta taaataagtc tataaatgaa
     9001 aatttaaact ttggagagta tggttctgta ttagggtcat taggacaaag tttatctgat
     9061 aatggattaa aagcatctgt attgggtaat tcagatataa ttgaaaatgg tcaacttata
     9121 aaaaatagaa acttatgtct aactgcaatg gatgaatatg gaagaatacc taatggaaat
     9181 gtagatacta taaataaaaa agacttatct atgccttatg gaatatcaac tgattatgat
     9241 aaattgattg tagagactaa agaagcttac aaaaataatg atgtgatttt tgtggagctt
     9301 ggagacacat atagattaga tttatataaa ccaaatttaa atgaaaaaac atatgagagt
     9361 atgaaagata acatagaaaa aaatatagat atatatttaa aaaatatatt ttctatggtt
     9421 gaagaaaatg acactgtcta catagcaagt gcatttccaa gtgatttaga ttataaaaat
     9481 aaaagaagac tttcgccaat aataaaatta aatggagaag ggaaaggtat attatcatct
     9541 tcaactacta gaagagaagg tattgtaaca aatttagatg ttggggttga aatattagac
     9601 aactttaata taaaaaatca aaatatggtt ggaagaaaat atgaacttat taacagagat
     9661 gataataaag aatttttgat ggatgaatat caaaagatag tatctatttc aagcataagg
     9721 tctacaatac tgaatggttt tgtaagtata gttttcttgt catggatagt tgctatgatt
     9781 gcaattttat ttagaaagca tatatcaaaa aattataaag aaacaacatt ttttatatta
     9841 aaggaacttt taaaaatagg aatagtaatg ccactgtcat ttatgataac accaataatg
     9901 aatttcaaaa caccattagc aatatcttta ggaataatta taataacact tactatatat
     9961 cttataagta aagttttaat aaagaacgat ttaaaaaaca tgctgttttt tactggatta
    10021 accatagtaa ttatggttat tgatgctggg tttggctcat atttaatgaa aagcaatgtt
    10081 atgagttatg attgtataat tggagctaga tattatggag taggaaatga gtatcaaggt
    10141 gtggcaatcg gtagtgcaat atttacattt gcaattttac ttacatataa aaatatacca
    10201 aaatggtcta taatagtttt ttctttggtt atcttaatta caagtgcatc tccaattatg
    10261 ggagcaaatg ttggaagtgc tatttctgaa tgtgttgcat ttttactatt tatactattg
    10321 atatataatg taaagataga tttcaaaaaa atcgtattat taggtatagc agtagtattt
    10381 gtattaggag tttttgtagc aatagatatg attttgggaa gtaattcaca tttaggcatg
    10441 tttgtaaaag aaatttactt taatggacca ggagaaataa tacaaacatt tagtagaaag
    10501 atagagatga atttaaagct agctcaaacc agtgcatggg taaatatact gttaactgga
    10561 ataggtgtaa ttttagttct tatgattaat cagattagat actttaagca actgatggat
    10621 gagtatccaa ttgtatttaa aggatttata gcatcaatag caggatgttt agtaactctt
    10681 ttagtgaatg actctgggat tgtgtcatca gctacagcct ttatttatgt aattgtacct
    10741 atgataacat taagtataaa tcttacagca ttaaaagaat aagtactgta tgaataatat
    10801 gtaaatatta atacattaat ttaacttctt tatttacaaa ataaagaaga aagtttataa
    10861 aaaataaatt ataataaaaa agagtttatc tcaaaattga ttattctaat caaattttaa
    10921 gataaactct tttttattat aaagataaat aacatataac ttcatatttt aggtagattt
    10981 tttcttatat gtttaggagt gcatttaaat ttttccttaa aaattctgta aaataatttt
    11041 gtattagtaa atccatgagc ttctaatata ctgcataagg tatcatttgt attaattaag
    11101 tctttataaa tatgagaaat acgcacatca tttaaatact ctaagtaagt ttgtcccata
    11161 tactgtttaa attttctaca aaaatattct ggttgtaagt gtgctacctt agcgattttg
    11221 ttaatagaaa taggcatttt gtaattaatc tttgtatatt ctaaaactgg ttcaaatttt
    11281 gacaaatcaa gtactgtttt tttcatattt ttattaccta cagatatttt aaagttagta
    11341 tataattcaa ataaaagttc aaataataag cttgtaaaga gcagattagc tgcaggagga
    11401 gaactaagtt taatttctct catgttttct aatataagtt taactttact tgaatttttt
    11461 ttatagttat tgtcattggc attaacctta aagtcaaaat agcaattttt aaaatcaggg
    11521 atatatttgt ttagaaaaga caatggtact tgcaataata tagaggtatt ttgataaata
    11581 catctagttg agtgaataat tccagaatta atgaacatac actcattagc ttttaaatta
    11641 taagtgtaac cctccatgtt aatttgtaaa tcaccagaag ttatataaat tatttctaat
    11701 gagttatgcc aatgatttgc tacataacta ccactatcat caaatagttt gaaattaaca
    11761 tctaaattat caataggttc aattatttca tgtgaaaaat tatttctgtt cataataata
    11821 tcctccttat aattttaaat tataaatagt aaatttataa tgttcaatat taa
//
LOCUS       DAMJVK010000109        11777 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.114, whole genome shotgun sequence.
ACCESSION   DAMJVK010000109 DAMJVK010000000
VERSION     DAMJVK010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11777
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.114"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(348..893)
                     /locus_tag="PC414_003161"
     CDS             complement(348..893)
                     /locus_tag="PC414_003161"
                     /inference="COORDINATES: protein motif:HMM:NF012798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF2465538.1"
                     /translation="MRVEPIENKKDIEKLKRYFKNKGKIRDLTLITLGLNIGFRIEDI
                     LKLKVKDVYKKREINIKESKTGKRIRKPINDSLKEILDIYCKDKPTNEYLFKSQKGEN
                     EAISTTQAYRIIKKASKACRIKLNIGTHSMRKTHGLHIFKDKGIEVAQRALNHANQID
                     TLTYIGVVQKQADDAIMKLNL"
     gene            complement(909..1157)
                     /locus_tag="PC414_003162"
     CDS             complement(909..1157)
                     /locus_tag="PC414_003162"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465539.1"
                     /translation="MEGTKRSYRKIPKLDLRKVKLADVFEKNEPGVFRVKESDEQLLY
                     DCLEETQNIRQEKHIAYAIRFRGKSVYCVFYESPFWDL"
     gene            complement(1513..2148)
                     /locus_tag="PC414_003163"
     CDS             complement(1513..2148)
                     /locus_tag="PC414_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF2465540.1"
                     /translation="MIIGYERVSTRKQDNSRQERVLENEGIKFDKIYKDVITGATKER
                     EALSRMLVEVNKGDTVYCESISRLGRNLKDLIDIIDTLVQKGVRVKILKEGIDTNNNT
                     YKMLLGIFGAVAEMERETIQERTQQKIDQLKEDKKNTGEINTKSGKWFGRQKVTKELL
                     ETNKEFIKYYNKILDGSLKKNEVAKILNVGRTTLYEWIKIYEGGEIKRNRS"
     gene            complement(2738..3106)
                     /locus_tag="PC414_003164"
     CDS             complement(2738..3106)
                     /locus_tag="PC414_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004068822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2465541.1"
                     /translation="MELDFKFIGQRIKIARIKKKLTQEVLSEKINVSPQHVSNIETGN
                     SSVSLPTLVAIANTLGVSVDELLCDTILSSKAVFENELEEVLRDCNEYEIRFITDIVK
                     ASKKSIRDIKLFQSNLNDRY"
     gene            3222..3554
                     /locus_tag="PC414_003165"
     CDS             3222..3554
                     /locus_tag="PC414_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2465542.1"
                     /translation="MDIPKIPQTELKVMKFIWSKNDIVTSKEVAKAMELEYSWKMTTT
                     LTILSRLVIKHFLASERIGRMTHYTILIAKKDYKISETKRFLNDIHSNSLESLLNSLK
                     DCNITHKK"
     gene            complement(3702..4139)
                     /locus_tag="PC414_003166"
     CDS             complement(3702..4139)
                     /locus_tag="PC414_003166"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465543.1"
                     /translation="MRVKNIISIFVCALIIIMSVNVVSFAEQFNSSDIIFMEDGKEEI
                     NPFSVYILNGRCNLSFSNGKANVNASVVGKSGVTRTSIVTKLQELKGGSWVNLKTSTK
                     KTGGKTCSVLNSYSVTKGHTYRAVAIITAENESKTLISTSQKY"
     gene            complement(4208..4909)
                     /locus_tag="PC414_003167"
     CDS             complement(4208..4909)
                     /locus_tag="PC414_003167"
                     /inference="COORDINATES: protein motif:HMM:NF025643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4367 domain-containing protein"
                     /protein_id="HDF2465544.1"
                     /translation="MNKDKLDIEKLLYEQLPKIEEKILYELPCDEDIDYEFSIEFESK
                     MDKIIKENEKKVKKNTYKNLKKIAIVFIIILSSIVTITTIKTEAFPENLIRIMKKVYK
                     EFTDYNFINPHKSNVSFKYIEPKYISTGYKEVERIEEENYLSILYKDKLDNYIIYQMM
                     LYSRDVIVLDTEDAFVENIKFNNQYAQYVEKGNTQQLLWHDKNNIYMLSKELPTKKIT
                     KKDKKEFIKIAKNIK"
     gene            complement(4902..5447)
                     /locus_tag="PC414_003168"
     CDS             complement(4902..5447)
                     /locus_tag="PC414_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2465545.1"
                     /translation="MLIYLAIIDSKEERIKFEEIYIKYKKLMFYVANQILKDDVLAED
                     AVHSSFLKIIDNLDKVNDVNSPKTKGFVVIIVKRISINIYNRRKKEEISNINDDNYKF
                     SSLDLSIEKLGECSYLGEALKKLSETDLQVILLKYSHGFSIREISKLLNIKEVNLYKR
                     NKRALEKLKKILSEMEALSNE"
     gene            5583..6311
                     /locus_tag="PC414_003169"
     CDS             5583..6311
                     /locus_tag="PC414_003169"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465546.1"
                     /translation="MKSKRINKMLATTMTMAMLAIPAVTATSYAMESTGLGTEYTYNN
                     ESKQQEEWVKSFDTETGKEILLNKTTNEKVSEVYENVNGKLVKLSINEALEKFNLNEE
                     IITKSPKSIFGVSSVKKITGSARKISQDVSGGKQGATISIGNSTTVTESFTVGGTTEA
                     IKAAVRANAGFTWSKSLTRSVTTTHKVPAGKIGYVAFKPYYKEVRGTVNGILAGTSIT
                     QKIVAKSPLKAGKVCDGLEYLAYK"
     gene            6337..6585
                     /locus_tag="PC414_003170"
     CDS             6337..6585
                     /locus_tag="PC414_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004035436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465547.1"
                     /translation="MENKNMYLRVSLILAVLGFIILFFNNDLALSLKATYLADKGFED
                     IVENQILKNYSYMFLIIGGVLFSIGIYNLTKLKQINKK"
     gene            complement(6771..9380)
                     /locus_tag="PC414_003171"
     CDS             complement(6771..9380)
                     /locus_tag="PC414_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011572948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF2465548.1"
                     /translation="MKIDIKQLIDIIKNANIDELYLRDIGVDTTQKRNVPCPIHGGKD
                     KNFQFDLNKRIYTCYSHNCVVGADIIELCRTYERFERPIEAILFLANKYNISLPINKS
                     KTNIKKTNNTIKNIYIKKKDINSFIEEKKQEAFKNNDIELAFEFECMTDKDKRKYYLD
                     NNIKIKSMLEKQNYSSYKADSIINIDKYISENLKGIRESIKHATEGKNVLMVAPTGSG
                     KTYSIINTLKELDIKALFIFPNSANVQQAIVEYKIAGAYDKIPTKHALEGNKLVAMTW
                     DKTEQLLKEDLSEYIIVIDEIHQTYTDAFRSKAIKSLNNITNKCRGRLDITATPSKLE
                     FEIYNKVIEYKQNIQTEYKVKLYDKINIGKVIEILNNSNNSMLLKDSISTLNYISKKV
                     NKKSGVVISDTKDTSKLYDRIVSYSDMEGYEVLLNTSVITAGVNINNPNVTDIIVIGE
                     KDVGKIKQYVARARGAKSINVHIFNSYKTTNEDSNVYSVEWCINENIKDVESLTNIYN
                     KASKRDLPYRAIGIDISPINIKNIDSNIYYDKKDMCYKTDKLYIKSNAYNNYYQTRTI
                     NSFKVLLEEYFNKIEIVETEKETKKIEEEIKEHEEAIKEFKKSAIEQLEGHKKYLVGY
                     SEIKKSMTSSNLLKYHHDMRIDNNICLKYYMEHDLYSLIINNNCRKTIDLFSDFVLDN
                     SYSIDLSWKLATMSDEKRNLFFEQINTTIYRKINKKYANSLLNDDNIGTYTYNYINFN
                     FGVGTSYTKEHLEALAVDLQTFLATKKKLTIKKISLILQSIFVIEVKQHRGVTGLPYK
                     YYKNILPNPVTDKKVIRVYTIKKHIDIEDIKKELGLIDNDLSIEHLVEARINKLLNAI
                     DETEKEVLLEGLI"
     gene            complement(9710..10012)
                     /locus_tag="PC414_003172"
     CDS             complement(9710..10012)
                     /locus_tag="PC414_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011572949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465549.1"
                     /translation="MLKDDLLRIGFDTYGVNLEDKLLENNEIYLNDKKESERIMNRLL
                     EILEEKEHKKMLIDFEEKVFGNSVIEKEYAYRMGVLDGLDLAEFIDLNKKLCLKRN"
     gene            10181..10384
                     /locus_tag="PC414_003173"
     CDS             10181..10384
                     /locus_tag="PC414_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003366523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2465550.1"
                     /translation="MDIQINKLLENQNKTRYWLAKQIGMTHQNLTKLANNNTNSIKFD
                     SLEKICMALKCSPNELFGWEQDK"
     gene            10464..11009
                     /locus_tag="PC414_003174"
     CDS             10464..11009
                     /locus_tag="PC414_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011572951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465551.1"
                     /translation="MDRYTFDNLPIKEQIQYINNKLLEGNTLTNTCKDIGIGRTTIRN
                     RFKQHGYEFNPTKKLYISIIEVVELESSSKVVAGDTSNDNTLVDATNIDFNNILSKFN
                     DMNNKLNEVYSWYESQSSNKVVGADKLNIDDFKGNIVVRSYKVYEDIQKEFVAFCEAN
                     KKYRVQDIISQALRDFMDKYK"
BASE COUNT         3901 a         1820 c         1281 g         4775 t
ORIGIN      
        1 aaaaaaaaat aaccatacgc attgatttta cttaaattaa aagtgttttt cgagttgttt
       61 tttcacttag tttagtaatt ttgaaatatt ataatattaa gcttcaatat gtatctatta
      121 tcatttaata atcaattaat aatagatatt ttttctaaac aagataaaaa caatatctat
      181 accagtaata gcaatagata gaagtatata aaaaaatgta cgattactat aaactattac
      241 atttatttta gttatcttta tccattgcaa ttacttatac ataagcttat ttacttaatc
      301 attaactata aaatgtaaaa acaacaaaga aaatcataca tttttaacta taaattaagt
      361 ttcataatgg catcatcagc ttgtttttgt actaccccaa tataagttaa tgtatctatt
      421 tgattagcat ggtttaaagc tctttgtgcg acttcaattc ctttatcctt aaatatatgt
      481 aaaccatgtg ttttcctcat gctatgagta cctatattta attttatcct acaagctttt
      541 gaagcttttt taataattct atatgcttgt gtggtgctta ttgcttcatt ctctcccttt
      601 tgacttttaa ataagtattc attagttggt ttatctttac aatatatatc taatatttct
      661 ttcaaactat catttatagg ctttcttatt cttttccctg tcttactttc ttttatattt
      721 atttctcttt ttttgtatac atctttaact tttagtttta aaatatcctc tatcctaaag
      781 ccaatattta accctaaagt tattagggtt aaatctctta ttttaccttt atttttaaaa
      841 tatcttttaa gcttctctat atctttttta ttttcaatag gttctactct catttaactc
      901 actcctattt ataaatccca aaatggactc tcataaaaaa cacaatatac actttttcct
      961 ctaaatctaa tcgcataagc aatgtgcttt tcttgtctaa tgttttgagt ttcttctaaa
     1021 caatcatata agagttgttc atcactttct tttactctaa aaactcctgg ctcattcttt
     1081 tcaaatacat ctgctaattt tacttttctt aaatctaatt ttggtatttt cctataactt
     1141 cttttagttc cttccataat ttcccctctc atttttctat ttatataaat tattttaata
     1201 tatttttaca atccaaaagt ctctattttg ttcaaaatat gtagtttttt attacttgtt
     1261 aatttacaaa atcatttgat gtgattttat cattctatct actataccaa aaaaaatctt
     1321 attttcttaa gtaaatcaaa atttaatatt tataataaaa aaaagagcct aaaaaattag
     1381 actcttgttt gcaaaagcta ttaaattgta aagctttcaa taatataatt atactatata
     1441 ttttgttctt attttgtcta attttgttca atttttgttc aaaataataa cacttaaata
     1501 tattataatc aattagcttc tattcctttt tatttctcca ccttcatata tctttatcca
     1561 ctcgtaaagt gtagtcctac caacatttaa tatttttgcc acttcattct tctttaagct
     1621 tccatcaagg attttattat aatattttat aaactctttg ttagtttcta aaagctcctt
     1681 cgttactttt tgtctaccga accattttcc actctttgta tttatctcgc cagtattttt
     1741 cttatcctct tttagttggt ctattttctg ctgtgttctc tcttgaattg tttctctctc
     1801 catttctgca actgccccaa atataccaag taacatcttg tatgtattat tatttgtgtc
     1861 tataccttct tttaatattt ttactcttac acctttttga actaatgtat ctataatatc
     1921 aattaaatct tttaaattcc ttcctaaacg gcttatagac tcacagtaaa cagtatcccc
     1981 cttatttact tctactaaca ttctgcttaa tgcttctctt tcctttgttg ctcctgttat
     2041 tacatcttta tatattttat cgaacttaat tccctcattc tctaaaactc tctcttgtct
     2101 gctgttatct tgttttcttg tacttaccct ttcatatcct attatcataa ccctaccccc
     2161 atttgtcttg ttataataat tatacaatat attttttgtt cggtcaaact atatttttca
     2221 tttttgcaat gccgaacata ttttattgtt gatttccaat gattctatgt aaaaatcata
     2281 caaatattgt gtgtacggtc aaacgttggt ttttccgaac acgaacatat cacctttttt
     2341 atatcaacta ttccttaaat attttatata aaatcgccaa aatcttttta tattttttag
     2401 gcaaccaagt ttttaaaaca aaaatggtat atcagtaaga attatgtttt cacacaatat
     2461 aattcttaat gatataccat ttaagaacct cataagaggt agatagagat aattacataa
     2521 tcatcacata cgttttatca aatctgtttt gtaatagggt ttatacttaa ataataacaa
     2581 aaataccgta ttttttctac atttatactt tttttgttga tagtaaaata tggaaattag
     2641 tcatattttc agttattaac tcataagttt atacaacttt tatttaaata taataaaaac
     2701 atcaataata attaaattat tgaagcatat atttatatta atatctatca tttaaatttg
     2761 attgaaacag ttttatatct ctaatggatt ttttagaagc ttttacaata tcagttataa
     2821 atcttatttc atactcatta caatccctca aaacttcttc taactcattt tcaaagacag
     2881 ctttagaact taagattgta tcacataata attcatcaac cgacacccca agagtatttg
     2941 caatagcaac gagcgttggc aaacttacac ttgaatttcc cgtttcaata ttgctgacat
     3001 gttgtggact aacattgatt ttttctgata aaacttcttg tgttagcttt ttcttgattc
     3061 ttgcaatttt tatcctttga cctataaatt tgaaatcaag ctccatatgc gtattccctt
     3121 cctacaagta ttcacacata ttgtaacctt tcccaaagta tattataata aattaataga
     3181 atgtttcatt atgatagtta tagtgtatag aaaggaatta tatggatata ccaaagatac
     3241 ctcaaacaga actcaaagtc atgaaattta tttggagcaa aaacgacatt gtaacatcta
     3301 aagaagttgc aaaagctatg gaacttgaat atagttggaa aatgacaaca accctaacaa
     3361 tcttatcaag gcttgttatt aaacactttt tagcatctga acgaataggc agaatgacac
     3421 attatacgat actcatagca aaaaaagatt ataaaatatc tgaaacaaaa agatttctaa
     3481 atgacataca ttcaaattca ttggaaagcc ttttaaactc tttgaaagat tgcaatatta
     3541 cacataaaaa atagtacatg agtataaatt atattattat acctatgtac tatttaagtt
     3601 tttctataat gttcctatat ctatctaatt ttgattgatt tagatacttc tgtagtagtt
     3661 ttcaaaacgt ttcttaactc ttgtaagtgt attttctttt tttaatattt ttgagatgta
     3721 cttattaatg tttttgactc attttcagca gtaattattg caacagctct ataagtatgt
     3781 ccttttgtca cagaatatga gtttaaaaca gaacaagttt ttcctccagt tttctttgta
     3841 gatgttttta aatttaccca acttccgcct tttaattctt gtaacttagt tacaatagat
     3901 gttctagtta ctccagattt tccaacaaca gaagcattta catttgcttt tccattagaa
     3961 aatgataaat tacatctacc atttaatatg taaactgaaa aaggatttat ttcttcttta
     4021 ccatcttcca taaaaataat atcagatgaa ttaaactgtt cagcaaaact aacaacattt
     4081 actgacatta ttattatcaa cgcacataca aatattgaga ttatattttt tactctcatt
     4141 aagtacctcc ttataaaatc ttatatattc aatataacaa ctaactagca ctattttaga
     4201 caaaaattta cttaatattt tttgcaattt taataaattc ttttttatct ttcttagtta
     4261 ttttttttgt aggtagctct ttagataaca tataaatatt atttttatca tgccacaata
     4321 attgttgagt atttcctttt tcaacatact gtgcatactg attattaaat tttatattct
     4381 caacaaaagc atcctctgta tccaagacta taacatccct agaatataac atcatttgat
     4441 aaattatata gttatctaat ttatctttat ataatattga tagataattt tcttcctcaa
     4501 ttctctcaac ttctttatat ccagtagaaa tgtatttagg ttctatatat ttaaatgata
     4561 catttgattt atgagggttt ataaaattat agtctgtaaa ttctttatat accttcttca
     4621 ttattctaat taaattttct ggaaaagctt ctgtttttat agtagttata gtgactatac
     4681 ttgataaaat tattataaaa acaatagcaa ttttttttaa gtttttatag gtgttctttt
     4741 ttactttttt ctcattttct tttataattt tatccatttt actttcaaat tctatagaaa
     4801 attcatagtc aatatcttca tcacaaggta attcatacag tattttttct tctatttttg
     4861 gaagctgttc gtataataat ttttctatgt ctaatttatc tttattcatt tgataatgcc
     4921 tccatttcgc ttaaaatttt ttttaatttt tctaatgctc ttttatttct cttatacaaa
     4981 ttaacctctt ttatattcag tagtttagaa atttctctga tagaaaaacc atgactatat
     5041 tttaagagaa tcacctgtaa atctgtctct gacagttttt ttaaagcttc tcctaaataa
     5101 ctacactcac ctaacttttc aatcgataaa tctaaagagc tgaatttata gttatcatca
     5161 ttaatattac ttatttcctc tttttttctt ctattatata tatttattga aatccttttt
     5221 actataataa caacaaaacc tttagttttt ggactattca catcatttac tttatcaaga
     5281 ttatcaatta tttttaaaaa tgaactatga acagcatcct ctgctaaaac atcatctttt
     5341 aaaatttgat tagctacata aaacattaat ttcttatatt taatatatat ttcttcaaat
     5401 ttaattcttt cttcttttga atctataata gctaaatata ttaacataaa ttattctccc
     5461 ttaaaaaata ttatccttaa aaaaattata tacaatttat aaaaaattat atataaatgt
     5521 ctaaaaataa aaacttcatt tgttatatat ataaataaaa acataaaagg atggtgtttt
     5581 atatgaaatc aaaaagaata aacaaaatgt tagcaactac aatgacaatg gcaatgctag
     5641 caataccagc agtaacagca acatcatatg caatggaatc aacagggtta ggaacagaat
     5701 atacatataa taatgaatct aaacagcaag aagagtgggt taaatcattc gatactgaaa
     5761 caggaaaaga aatattatta aataaaacta ctaacgaaaa agtttctgaa gtatatgaaa
     5821 atgtaaatgg aaaattggtt aaattaagta ttaatgaagc actagaaaaa tttaatttaa
     5881 atgaggaaat tatcacaaaa tctcctaagt ctatttttgg agtatcaagt gttaaaaaaa
     5941 ttactggttc tgcacgcaaa atatcacaag acgtatcagg tggaaaacaa ggtgctacta
     6001 tatctatagg aaattcaaca actgtaactg aaagttttac tgttggtgga actacagaag
     6061 caataaaggc agcagtaaga gcaaatgcag gatttacatg gagcaaatct ttaacaagaa
     6121 gtgtcacaac tacacacaaa gtacctgcag gtaaaatagg atatgtagca tttaaaccat
     6181 attataaaga agtaagaggt acagtaaatg ggatacttgc aggaacttct ataactcaaa
     6241 aaatagtagc aaaaagtcct cttaaagcag gaaaagtttg tgatgggtta gaatatcttg
     6301 catacaaata ataaataata gaaagagttg attaaaatgg aaaataaaaa tatgtactta
     6361 agggttagtt taatattagc agtattaggc tttataatat tattttttaa taatgactta
     6421 gcattatcac taaaagcaac atacttagct gataaaggct tcgaagacat agttgaaaat
     6481 caaatattaa aaaactattc ctatatgttt ttaataatag gtggtgtact attttccata
     6541 ggaatctata acttaaccaa actgaaacaa attaataaaa aataaataaa ataagaggac
     6601 ttataaatta taagtccttt tattttacac ttataagcta ctttcaacct ttcatgattt
     6661 aacctttatt tatacaacac ccaaaactcg acctcaaaaa gagaacagga cttcgtcccc
     6721 cttaatacaa agcaacattt tttagtacta ttcgtactta agattagcta ttagattaat
     6781 ccttccaaaa gaacctcttt ttccgtttca tcaatcgcat ttaaaagttt atttattcta
     6841 gcttcaacta aatgctctat gcttaaatca ttgtctatta gtcctaattc tttttttatg
     6901 tcctctatgt ctatatgttt cttaatagtg taaactctaa ttactttttt atctgtcaca
     6961 gggtttggaa gtatattttt atagtattta tagggcaacc ctgtgacacc cctgtgttgt
     7021 ttgacttcaa taacaaaaat tgattgtaat attagactta ttttcttaat agttagcttt
     7081 ttcttagttg ctaaaaacgt ctgtaaatca acggctagag cttctaagtg ttcttttgta
     7141 taacttgtac caactccaaa attgaagttt atataattgt aagtataagt accaatatta
     7201 tcatcattta gcaaagaatt tgcgtatttt ttattaattt ttcgataaat tgttgtattt
     7261 atctgttcaa aaaatagatt tcttttctcg tctgacattg tcgcaagttt ccaagatagg
     7321 tcaatactat aactattatc taatacaaaa tcactaaata agtctatcgt ttttctacaa
     7381 ttattattaa ttattaggct atataaatca tgttccatat aatattttaa acagatatta
     7441 ttatctatcc tcatgtcatg atgatatttt aataagttac tgctggtcat tgacttctta
     7501 atttcactat agccaactag atacttttta tgtccttcta gttgttctat agctgatttc
     7561 ttaaattcct taatagcttc ctcatgctct tttatttctt cctctatttt tttagtttct
     7621 ttttcagttt ctactatctc aattttatta aaatattcct ctaatagcac tttgaagctg
     7681 tttattgttc ttgtttggta atagttattg taagcattac tttttatgta taacttgtcc
     7741 gttttatagc acatatcttt tttatcatag tatatattac tatcaatgtt ttttatgttt
     7801 atagggctta tatcaattcc tatagctcta tatggtaagt ctctcttaga agctttattg
     7861 tatatatttg ttaggctctc aacgtctttt atgttctcat ttatgcacca ttcaaccgaa
     7921 tatacattgc tatcctcatt tgtcgttttg taactattaa aaatatgtac atttatactt
     7981 tttgcccctc tagctcttgc aacatactgt tttatttttc ctacatcctt ttctccaatt
     8041 acaataatat ctgttacatt aggattattt atgtttacac ctgccgtgat tacagacgta
     8101 tttaaaagca cctcatagcc ttccatatcg gagtaactaa caattctatc atatagtttg
     8161 ctagtgtctt tagtatctga aataacaact ccactctttt tattaacttt ctttgatata
     8221 tagttaagtg ttgatatact atcttttaaa agcattgaat tatttgaatt atttagtatc
     8281 tctattactt tcccaatatt gattttatcg tataacttaa ctttgtattc cgtttgtata
     8341 ttttgtttat attcaataac tttgttatat atctcaaatt ctaatttaga gggtgttgca
     8401 gttatatcta gtctacctct gcacttatta gttatattat ttaagctctt tattgctttg
     8461 cttctgaacg catctgtgta agtctgatgt atttcatcaa taactattat gtactcgctc
     8521 aaatcctctt ttagaagctg ttcagtctta tcccacgtca tagccactaa cttgtttccc
     8581 tctaaagcgt gtttagtagg tatcttatca tatgcaccag ctattttata ttcgactatt
     8641 gcttgttgca cattcgcaga gttggggaat ataaagagtg cttttatatc cagctctttt
     8701 aatgtattta taattgagta tgtttttcca cttcctgtag gagctaccat taatacgttc
     8761 ttaccttctg tagcatgttt aattgactct ctaatacctt ttagattttc acttatgtat
     8821 ttatctatat taattatcga atcagcttta taacttgaat agttttgttt ttctagcatg
     8881 gattttattt taatattgtt atctaagtaa tactttcttt tgtccttatc agtcatacac
     8941 tcaaattcaa aagctaactc aatatcattg tttttaaaag cttcttgttt cttttcttct
     9001 atgaagctat ttatatcttt tttctttatg taaatgtttt taatagtatt atttgttttt
     9061 ttaatatttg tcttactctt gttaattggt aatgagatat tatatttatt ggctaggaat
     9121 aaaatagctt ctataggtct ttcaaagcgt tcatatgtgc gacataattc aattatatca
     9181 gcaccaacaa cacaattatg gctatagcat gtatatattc ttttgtttaa atcgaattgg
     9241 aagtttttat ccttaccacc atgaattggg caaggaacat tccttttttg agttgtatcg
     9301 actcctatat ctcttaaata tagttcatct atattggcat tttttattat atcaattaat
     9361 tgttttatat ctattttcat ttgtaaaaat cccccttttt tagttagggg ctaatcaagt
     9421 tttatcaagc tatgtattta cattctgccg taaatctcgt ataattaaca tataaaagaa
     9481 taaggaaata tatattttat atgttaatta agcctaagtg attagctcct agtattaacc
     9541 cctagtggct aactcctaat cttgattaac ccttaaatgg aaagaatgag tggatgccgt
     9601 caaacaaata ctcgttcttt ttcgtttttt ttgttattag tattatatca ctattaaagt
     9661 tataattcat tgtttagaat tatttttctg acggttttaa tatatatttt taatttcttt
     9721 tcaagcataa ttttttattt aagtctataa attcagctag gtcaagccca tctaaaaccc
     9781 ccattcggta tgcgtattct ttttcaatta cactgttacc aaatactttt tcctcaaaat
     9841 caattagcat ttttttatgt tctttttctt ccaatatttc taacaatctg ttcattattc
     9901 tttcagattc ttttttgtca tttaaatata tttcgttatt ttctagtagt ttatcttcta
     9961 aatttactcc gtatgtgtca aatccaattc ttaataaatc atcttttaac attactttta
    10021 acactccttt ttatgcacac ttaaatcttt tgcaccccta aatatggtaa tatattctat
    10081 ttattattat gtaattattt actatgtatt gtcaagcata tatattgttt ttggtacata
    10141 tttccgatac tatataataa aaaaggtggc gattaaattg ttggatatac aaataaataa
    10201 attattggaa aatcaaaata aaacacgtta ttggttagca aagcaaattg gaatgactca
    10261 tcaaaactta actaaactag ccaacaataa tacaaattct attaaatttg attctttaga
    10321 aaaaatttgt atggctctaa aatgttctcc taacgagctg ttcggttggg agcaagacaa
    10381 ataaaaaaat ataaatccta aagggtttat ttataactac tagaaaacta ctttagtagt
    10441 tattttattt tggaggttta aacatggata gatatacatt tgataactta cctatcaagg
    10501 agcaaataca atatattaat aacaagctct tagagggcaa tacattaact aacacatgta
    10561 aagatattgg tattggtcgt acaactataa gaaatagatt taaacaacat ggatatgaat
    10621 ttaatccaac taagaagcta tatatatcta ttattgaggt tgtcgaatta gaaagtagtt
    10681 caaaagtagt tgcgggcgac actagcaatg acaatacttt agttgatgca accaatattg
    10741 attttaataa cattttatct aaattcaatg atatgaataa taaactaaat gaagtatata
    10801 gttggtacga atcacaaagt agtaataaag tagttggggc tgataaacta aatatagacg
    10861 attttaaagg caatatagtt gttagaagct ataaggtcta tgaggatatt caaaaagagt
    10921 ttgtagcctt ctgtgaagct aataagaagt acagggtaca agatattatc agccaagctc
    10981 ttagggattt tatggataaa tataagtagt tttacttact ttctgatatt ctaaaaactt
    11041 ttcaatgaaa atctcatttt ttggcttcct gtgaaaaata tgtactcctt ccagtcctct
    11101 atctttattt actttattaa tccgataggg ggcgtattat atatatttct atcgagcata
    11161 tactcatcaa aacatagcat tagtttgatt attatatttc tatttatttt aagattatat
    11221 atacaatttg ccccttaata cattgcaatt actaacttct ttagttttga tattaatatt
    11281 taaatcattc ctttttaaag gtgctataag tgctatatag gcattgcaac ctatatactt
    11341 tttaggggtg cagttgcata tgcaagctct ttatttccca acacttaacc agttataaat
    11401 atttatatcc tttattagcc aaatatagca ttttaaacaa ctccgactat ctttagtagt
    11461 aacattttac atagctttat taatgtattt ttgcatctgc aaccatgttt ttaagttgca
    11521 acaccccacc cccacacctt acacaaaaca atacttatct ccgaagccat atacctacct
    11581 cggttcacat atttttcttc aacagcgtta ttttcagcaa ggggggctaa acattatact
    11641 ttttttcagt gcattaaata gccttacatg tattggtata actttatttc aaacccttaa
    11701 ttgaattgtt tcaatctaaa ttaagtaaat tttaataaga taaaatatat agtttgtaac
    11761 tttatttcaa aatacag
//
LOCUS       DAMJVK010000110        11359 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.115, whole genome shotgun sequence.
ACCESSION   DAMJVK010000110 DAMJVK010000000
VERSION     DAMJVK010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11359
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.115"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(23..211)
                     /locus_tag="PC414_003175"
     CDS             complement(23..211)
                     /locus_tag="PC414_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465552.1"
                     /translation="MESGLFVSRILCVVLSFPHFKKIADGFTDEIALYFPHKTNIYLK
                     IFLRFLLLNYILNKENIK"
     gene            707..865
                     /locus_tag="PC414_003176"
     CDS             707..865
                     /locus_tag="PC414_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465553.1"
                     /translation="MRITKGKIKELIELCHQLEKLFVQVFKSLEHILISLCSCIGWLL
                     ILIYIIKH"
     gene            1118..1309
                     /locus_tag="PC414_003177"
     CDS             1118..1309
                     /locus_tag="PC414_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2465554.1"
                     /translation="MKLEKLIDIRKEKKYSQEDIARMLDISLRNYHRKEKGENQFTVS
                     ELEKICSYLDIESKELLDI"
     gene            complement(1523..1834)
                     /locus_tag="PC414_003178"
     CDS             complement(1523..1834)
                     /locus_tag="PC414_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2465555.1"
                     /translation="MNRLKEVRKERGYNIQEIADKLNVAYNSVLRWENGNRKIDIDKL
                     VELSNIYNVSIDYILCKTDLKENIQLKEEEVNLLDNYRELDTKSKTIVQEQINTLKKL
                     L"
     gene            2563..2886
                     /locus_tag="PC414_003179"
     CDS             2563..2886
                     /locus_tag="PC414_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465556.1"
                     /translation="MDEKILELLQKMDTSINDIQGEIKWINTRLDSMDGRFDGIENQL
                     SDLKEGQEEIKKKLDLTYNQVARNMEGITEVGEKIDTLKNDMNFVEMATSKNWNEIAK
                     LKSIK"
     gene            2898..3131
                     /locus_tag="PC414_003180"
     CDS             2898..3131
                     /locus_tag="PC414_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465557.1"
                     /translation="MDEKTLELLREIQESIKILNDKLDEFDYTQDKIKSNVEGLLECF
                     SRIDFRIEDLENGQTSLYTKLDIVQNETAKSIK"
     gene            3226..3414
                     /locus_tag="PC414_003181"
     CDS             3226..3414
                     /locus_tag="PC414_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465558.1"
                     /translation="MYEQRKAKVIFNKGAGNSRGESYTNRVTIPTTWIKHMDITKLDR
                     EVLLTFDGNKIVIEKIDE"
     gene            3535..4248
                     /locus_tag="PC414_003182"
     CDS             3535..4248
                     /locus_tag="PC414_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDF2465559.1"
                     /translation="MLNIYITNLGKYNEGELIGEWAEFPVNEEELEEILDRIGINEEY
                     EEYFITDFETDIEGLEISEHSNIEQLNELAKELEELEEYEIEQLKALLGCGYIDFYDM
                     LEGDICRLSGNYTFIQLDDRSPLDDENLGRSYIEEICCGDLSCIENIAYYFDIEAFSR
                     DLRFDRDMIIENLYEEDIEYYENMTDIEFAENYIERLGDIEELGQETLERYFNYELYG
                     TDLRYENICIASNYLAIVS"
     gene            4316..4456
                     /locus_tag="PC414_003183"
     CDS             4316..4456
                     /locus_tag="PC414_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465560.1"
                     /translation="MKKLIIKIKSLFKKDLDSSYWSTTESIILKCGNSNQLELLEQAN
                     KL"
     gene            4736..5431
                     /locus_tag="PC414_003184"
     CDS             4736..5431
                     /locus_tag="PC414_003184"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465561.1"
                     /translation="MNKIIVFYDIEGHPGFQVLVRNVKKYTRQNIIFELVEDYIKNNI
                     GEIDLKDEKIYSSVSWKVWTISDVAIYNTLFDLRLDYNKLIESEEEDTDNKECVKELF
                     CMGKGCKWMETVLLLRQNKIDSSQYNILTINNYTYVKTWAEQCKKILLANNYSKVFKF
                     NEIMKDCDNNLIGDICFKYNNKEYKHKFTFNIKKDKLVNIDCEYKIPFLRYVFNDIEK
                     FLTRYSENISNLK"
     gene            5571..5756
                     /locus_tag="PC414_003185"
     CDS             5571..5756
                     /locus_tag="PC414_003185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465562.1"
                     /translation="MQELCSMGLTYPFDFGRGKGKYQTNIDEETIYKRAKENTKIQEY
                     SKKYLNYIAKIKKSISK"
     gene            5790..6086
                     /locus_tag="PC414_003186"
     CDS             5790..6086
                     /locus_tag="PC414_003186"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465563.1"
                     /translation="MKKIINGYSIYKDGTWCEVIKEGKFIFAGSVNEKLSYEDIYIAL
                     TAKLEFRLHGKLLLSYSLYGTFQGEKQSTLELLAYENNCNINDIEVKQVVENIQ"
     gene            6223..6768
                     /locus_tag="PC414_003187"
     CDS             6223..6768
                     /locus_tag="PC414_003187"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465564.1"
                     /translation="MKKIANLNKEELKIIWEKNSQLREDVRKACEDNDMYWIDEILCH
                     LNKSLDDWSIGFYNDNYIKIEDNHKFLLELKSVCEKFSFLSEEEQRPLEYAISLIDKL
                     HCMDFENKRFNMLENKINNMVKKLEEKVIEEFNKMTEPLDKEYLLENFIEFYVEAYLN
                     DNEFYIDNEYVLYEKIIKSYA"
     gene            6794..7057
                     /locus_tag="PC414_003188"
     CDS             6794..7057
                     /locus_tag="PC414_003188"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465565.1"
                     /translation="MLANQIKGIKTSLETSSLRELKGYIKDLRKALSGYKYKNKIDEI
                     TEYQIDKLCYKIDNTDIFDLKYKEIEKYIDSLILILDFIFMEM"
     gene            7095..7679
                     /locus_tag="PC414_003189"
     CDS             7095..7679
                     /locus_tag="PC414_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465566.1"
                     /translation="MNEYILISYTKGEGTCDHCGRTIKNIATIKNNVTGEVFHVGLTC
                     VEKIMKLNVTFYKALSREIKKHYKCMEYYSKGLDIETNLNKIVKNNTKYKEGSYAYKS
                     NEQMLEDAIAEVAWSLARMIDSCMRMNKLSKSGLIDIDILNSLFIKYKEYMSKFDENY
                     NKNKYKYKSCYTMKPLKVILDENEDLKELYKSMQ"
     gene            7983..8504
                     /locus_tag="PC414_003190"
     CDS             7983..8504
                     /locus_tag="PC414_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465567.1"
                     /translation="MKFKNIVMGMLLGLIIVGVSGVTKVKAAELDNFYIESTDRVIEV
                     LEDESIVLYDTKEQVYNFYPICMGDWNYSFKNKKDLDSAVATYKELSSNINHLSNIYV
                     VNKLNDNGNIKVFLNDGSSIVYIKQDNKYYFYPSCMGDWYLTLDSKIYLTNCINTYIE
                     YYYSLSTGGGTNE"
     gene            8497..8727
                     /locus_tag="PC414_003191"
     CDS             8497..8727
                     /locus_tag="PC414_003191"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465568.1"
                     /translation="MNNNDKLSILNQLEYIENDIDARNEMIEKFKDLLVKYPKSRDIR
                     RYIKQQTQFLNGIRYIYDNLDILKNMINNDKK"
     gene            8898..9707
                     /locus_tag="PC414_003192"
     CDS             8898..9707
                     /locus_tag="PC414_003192"
                     /inference="COORDINATES: protein motif:HMM:NF022027.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HDF2465569.1"
                     /translation="MNKLKVKGKQKFMNLEIPVIEGGFGEDKKCMTDKTISEIHNQPN
                     FKIRERIKDNIKRFKEDIDYIDLKVIRDTDDNLELLQTLGYSKMQISKADNIFLLSER
                     GYSKLIKIMDTDLAWEIHDKIMDEYFSMRKKLNDPFENLSPELKVIIKLEQRQAELES
                     GQHELEEKVNVLYDFSTIDYNQQETIRSLVNRMVIQVYQCLNFSAYEDAKLRKRVYFS
                     LWKDYRKKFGVNSYKNTAKKNYDNAIKFIENWEPNYDLSNEIYMFQKVENI"
     gene            9985..10701
                     /locus_tag="PC414_003193"
     CDS             9985..10701
                     /locus_tag="PC414_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDF2465570.1"
                     /translation="MNILIENKFIEKLKYTKNIPNSLYKDLRHNFLFHSNKIEGSTFT
                     SESLQDLIEHNIVKGDHTLDDVITTVNSSYVFETVIDTLGEYLTEKYIKDLHGQLMYN
                     SNIHNMGLSGIYKKIPNRIIGTNSVLAQPFEVEPKMLELIEWYNNLKDITLKDLAEFH
                     YRFEIIHPFQDGNGRIGRFILLKQILENNLKLIIISSDTVDDYRQALKGCHNEYYKPL
                     EQYFESLDDYKEKNKDLWSF"
     gene            complement(10943..11242)
                     /locus_tag="PC414_003194"
     CDS             complement(10943..11242)
                     /locus_tag="PC414_003194"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465571.1"
                     /translation="MVIKVNFGGNYSPDYIYVPNEISLKKYEIRNEFHIWIDNTEDEH
                     PFWCYENGVQIGLHYRGDAIVYWLNNYILKNANDKARLIESFSNKKLDYDLIINL"
BASE COUNT         4779 a         1124 c         1706 g         3750 t
ORIGIN      
        1 tatgtaatca atacatacac ttttatttta tattttcctt gttaagaatg taatttaaga
       61 gtaaaaatct aagaaatatc tttagataaa tatttgtttt atgaggaaaa tacagagcta
      121 tttcgtctgt aaatccgtct gcaatttttt tgaaatgtgg aaatgaaagg actacacaca
      181 aaatcctact gacaaacaag ccactttcca taaaataccc cctacttcat ttttatttat
      241 tttaatatgt aaaacactct atttacttta tttaattaat aaaagtatta cttttatctt
      301 attttaaaaa tctatgtttt tgtcagcaaa aaatggcata aaattaagta ttttatgcca
      361 tttttctaaa acctagtatt ttaaacactt acaacaccta caccttctat tttgtcagta
      421 aaatgtcagt cattataatt cttagactac aaacctgttg taatcactgc atttcaattt
      481 ttagttattg atatctatat aaaatattat ttgctataga ataaattttt ttgttatgga
      541 ttataaagtc ttatatacat cattttagca ttaatctgac atcgtcgaat atcaactcac
      601 aacctcattg ccatattttt cttgacaaat ttgtcattat tgtatataat ttaattaaga
      661 taagaattaa tcttatctat acaattcaat attagggggt gacgttatga gaataacaaa
      721 aggcaaaata aaagagctaa tcgagttgtg ccaccaactt gaaaagctct ttgtacaagt
      781 atttaaatca cttgaacata ttttgatttc tttatgttct tgtatcggtt ggttattaat
      841 acttatatac ataattaaac attaataacc aatgcaatat acctataaaa atatatctct
      901 ataggtatat tgtaacacac ctaatattga aagtaaacga ggtgacgaca tgagtcaaca
      961 tattaaaaac actttaaaaa taataaagat actaggttta ttattcttct tatattttgc
     1021 acttagagca atatatattg tttatcttta atatctttat tgagttgctt aatgtataag
     1081 taactctttt ttgttataat attaatgagg tgatagcatg aaattagaaa aattaattga
     1141 tataagaaaa gaaaaaaaat attctcaaga ggatattgct agaatgttag acatatcttt
     1201 aagaaattat cacagaaaag aaaaaggaga gaatcaattt acagtaagtg aactagaaaa
     1261 aatatgtagc tatttagata tagaatcaaa agaactactt gacatatagt aatataatct
     1321 actcaaggta gtacttaaca ttaaataaaa taatttaata atataaaata gactagctat
     1381 aatctagtct attttattat aataacatat tgtacaagcc atttaaatcc attctaagca
     1441 catttcaatg cactcacctt atgtttatac ctttaatctt taaaattaaa tataaaaata
     1501 agctagagta tatatgtcta gcttacagta gtttttttaa tgtgtttatt tgttcttgaa
     1561 ctatcgtttt actcttagta tctaactctc tataattgtc taatagattg acttcttctt
     1621 cttttagttg tatattctct tttaaatctg ttttacacaa tatataatct atacttacat
     1681 tgtatatatt agacaactca actaatttat ctatatctat ttttctatta ccattttccc
     1741 accttaagac actattataa gctacattta gtttatcggc tatttcttgt atattatagc
     1801 ctctttcttt tctaacttct tttagtctat tcattttctc accttctttt ttacatttat
     1861 aatatcatat aagataactt taaacaacac caaaaaggtt agtactataa aacattcaaa
     1921 cccttttcgg tattattttt tattttttat attgacatac accctttttg gttgtataat
     1981 tgaatcatag caaaggagaa caagaaaaca caaagccacc tctcaaatga agtaacaggc
     2041 aaagaacctt cttaaaactc taaagctaat atcttaaagg attctattaa aaaatattaa
     2101 actactatta aaaaattact acctttaagt tgacctttta aatggtgtga atggtgaaag
     2161 gcacacaaca caaatattac acatagtttt ttcttcattt tttaactaca aaaatgaatt
     2221 agtcatacca gttggtgagg tgttcccaac attaagtaat taaataaata aaattaataa
     2281 aggtttccaa ttaaagccta caaaaaattg aggtatcaac ttagttgctt ctataatggc
     2341 actcaataca atttaatatg acatagcagt tgtgaggtac tgcgttataa aaagtactaa
     2401 agattgagca ttaaaattat agtttgtact gcttgacacc tacatggtta taaactatct
     2461 aatatttact aaaattattt attatttatt agattatttt agtaagtgtt agaatacaac
     2521 acttaaatta tgatataata ataaaatagg agtgtgaacg gcatggatga aaaaatatta
     2581 gaattattac aaaaaatgga tactagtatt aatgatatac aaggagaaat aaaatggatt
     2641 aatactagac tagatagtat ggatggtcgt tttgatggga ttgaaaacca acttagtgat
     2701 ttaaaagaag gacaagaaga aattaaaaag aaactagact taacttataa tcaagttgct
     2761 agaaatatgg aaggtataac agaagttggc gaaaaaatag acactttaaa aaatgacatg
     2821 aactttgtag aaatggctac atctaaaaat tggaatgaaa tagcaaagtt aaaatcaatt
     2881 aaataggagt agataaaatg gatgagaaaa cattagaatt gctacgagaa atacaggaaa
     2941 gtattaaaat tttaaatgac aaacttgacg aatttgatta tacacaagat aaaataaaat
     3001 ctaatgttga gggactttta gaatgtttta gtcgtattga ttttagaatt gaagacctag
     3061 aaaatggtca aacctcattg tatactaagc ttgatatagt gcaaaatgaa acagcaaaat
     3121 caattaaata gcagtacata aaaagacact tgaattattg agtgtctttt ctacaagctc
     3181 ttagtatggt attattaaac aataatatca ttggaggtat aacgaatgta tgagcagaga
     3241 aaagcaaaag taatattcaa taaaggagct ggtaattcta gaggagaatc atatacgaat
     3301 agggttacaa ttccaactac atggataaaa catatggaca taactaaact agacagggaa
     3361 gttttgttga cttttgatgg taataaaata gtaatagaaa aaatagatga gtagataaaa
     3421 aatctactca aaaaaatact tgacaactcg tgacgagtcg attataatta aatcataaaa
     3481 taaaattttt tacaaaatga ctagtaacga gtcagtaaaa agggaggata taaaatgcta
     3541 aatatataca taactaacct aggaaaatat aacgaaggtg aactaatagg ggaatgggct
     3601 gaatttccag taaacgagga agaattagaa gaaatattag atagaatagg gataaatgaa
     3661 gaatatgagg agtatttcat aacagatttt gaaacagata tagaagggtt agaaataagt
     3721 gaacatagca atatagagca actaaatgaa ttagcaaaag agcttgaaga gttagaagaa
     3781 tatgagattg aacaattaaa ggctttactt ggatgtggat acattgattt ttatgacatg
     3841 cttgaagggg atatttgcag attatcaggt aattatactt ttatacaact tgatgacagg
     3901 tcaccattgg atgatgaaaa tttaggacgt agttacatag aagaaatatg ttgtggtgat
     3961 ttatcttgta tagaaaatat tgcttactac tttgacatag aagcattttc aagagattta
     4021 cgttttgata gagatatgat tattgaaaat ttatacgaag aagatataga atattatgaa
     4081 aatatgacag atattgaatt tgcagagaat tatattgaac gattaggaga tattgaagag
     4141 ttaggtcaag aaacacttga aagatacttt aattatgagc tatatggaac tgacttacgt
     4201 tatgagaata tttgtatagc atcaaattat ttagctatag tttcttaaat aaaaagtttt
     4261 agggcgttct ttaaaaacgc cacttccaat aaaaaaattg aaaggggaat aaatcatgaa
     4321 aaaattaata attaagataa aatcattgtt taaaaaggat ttagatagta gctattggag
     4381 tactacagaa agtattattt taaaatgtgg aaacagcaat caattagaat tattggaaca
     4441 agctaataaa ttataaggag gtaataacat ggtcaattat tctgaatata tgacacagat
     4501 ttaataaata tatgtgacct tttagaaggt gaaataatag gagaagcagg aacttataat
     4561 tctttaagta ctagactagg aaatttaatc ggattagatt gggctttaaa aggagaagaa
     4621 ttgagacagc aattaataca aaatattaat aatatgatgt atttagataa tgaaaataaa
     4681 attcaatata aagaatttta agtaaaaaat aattaattta taggaggaaa ataaaatgaa
     4741 taaaataatc gtattttatg atatagaagg gcatccaggt tttcaagttc ttgtaagaaa
     4801 tgtaaaaaaa tatactagac aaaatataat atttgaattg gtagaggatt atattaaaaa
     4861 taatataggt gaaattgatt taaaagatga aaaaatatat agtagtgtaa gttggaaggt
     4921 ttggactata tcagacgtag cgatatataa taccttattt gatttaagat tagattacaa
     4981 taaattaatt gaaagcgaag aagaggacac tgataataag gaatgtgtaa aagaattgtt
     5041 ttgtatgggt aaaggatgta aatggatgga aacagtttta ttattaagac aaaataaaat
     5101 tgactcttct caatataata ttttaacaat aaataattat acatatgtca aaacatgggc
     5161 agagcaatgc aaaaaaatat tattggctaa taactattct aaagtattta aatttaatga
     5221 gataatgaag gattgtgaca ataatttaat tggggatata tgttttaaat ataataacaa
     5281 ggaatataag cataagttta catttaatat aaaaaaagat aagttagtaa atatagattg
     5341 tgaatataaa ataccttttt taagatatgt ttttaatgat atagaaaaat ttttaacaag
     5401 atattcagaa aatataagta acttaaaata acaggaggga aaatatgaat aaaacaattg
     5461 aaaacaattt aaatatagag cttaacaata ctatagaaca cttaatagga ataggttttt
     5521 gagaacaaaa atattataaa ccaactgtat ggaaatatgc aaatttagta atgcaagaat
     5581 tatgcagtat ggggcttaca tatccttttg attttggaag aggtaaggga aagtatcaaa
     5641 caaatattga tgaagagaca atttataaaa gagctaaaga aaatacaaag atacaagaat
     5701 atagtaaaaa gtatttaaac tatattgcta aaattaaaaa aagtataagt aaataaaatt
     5761 caacttttaa tataattgga ggtaataaaa tgaaaaaaat aataaatgga tattcaatat
     5821 ataaagatgg aacatggtgc gaagtaataa aagaaggaaa atttatcttt gctggttctg
     5881 taaatgaaaa actatcatat gaagatatat atatagcttt aactgcaaag ttagaattta
     5941 gattacatgg taagttacta ctttcatata gtctgtatgg tacatttcaa ggggagaaac
     6001 agtccacatt agaactttta gcatatgaaa ataattgtaa tataaatgac attgaagtta
     6061 aacaagttgt tgaaaatata caatagtctg caaattcaaa aaggaaacaa aatattaaat
     6121 ttaatagatt acataagtta ctatcattgt cgatataaag ttatatatta attgataatg
     6181 atagtaattt gataaaacaa acatattagg ggaggtataa aaatgaaaaa aatagctaat
     6241 ttgaataaag aggaattaaa aataatatgg gagaaaaata gtcaactaag ggaggatgta
     6301 aggaaagctt gcgaagacaa tgacatgtat tggatagatg aaatattatg ccatcttaat
     6361 aaatcattag atgattggtc tattggattt tacaatgata attatataaa aatagaggat
     6421 aatcataaat ttttattaga acttaaatct gtatgtgaaa aattttcttt tttatcagaa
     6481 gaagaacaga gacctctcga gtatgcgatt tcattaattg ataaattaca ttgtatggat
     6541 tttgaaaata aaagatttaa tatgttggaa aataaaatta ataatatggt caaaaaactt
     6601 gaagaaaaag taattgaaga atttaataag atgacagagc cattggacaa agaatattta
     6661 ttagaaaatt ttattgaatt ttatgttgaa gcctatctca atgataatga attttatata
     6721 gataatgagt atgtgttata cgaaaaaatt attaaaagtt atgcttaaaa gaatgtaatt
     6781 ggagggtttt aaaatgttag caaatcagat aaaaggtatt aaaacatcac ttgaaacaag
     6841 cagcttaaga gaactaaaag gatacataaa agatttaaga aaagcactat caggatataa
     6901 atataagaat aaaatagatg aaattacaga atatcaaata gataaattat gttacaaaat
     6961 tgataataca gatatatttg acttgaaata taaagaaata gaaaagtata tagatagttt
     7021 aattttgata cttgatttta tctttatgga aatgtaatca aaagaatgat tttaatataa
     7081 ctggaggttt aagcatgaat gagtatattt tgataagtta tacaaaaggc gaaggaactt
     7141 gtgaccactg tggaagaact ataaaaaata tagctacaat aaaaaataat gttacaggag
     7201 aagtttttca tgttggatta acatgtgtag aaaaaataat gaaattgaat gttacatttt
     7261 acaaagcatt gtcgagagag ataaaaaaac attacaaatg catggagtac tacagtaagg
     7321 gtttagatat agagacaaat cttaataaaa tagtgaagaa taatactaaa tacaaagaag
     7381 gttcttatgc ttataaatct aatgaacaaa tgttagaaga tgcaattgca gaagtggctt
     7441 ggtctttggc tcgtatgata gatagttgta tgagaatgaa taaacttagt aagtctggat
     7501 tgatagatat agatatattg aacagtttgt ttatcaagta taaggaatat atgagtaaat
     7561 ttgatgaaaa ttacaataaa aacaaatata aatataaaag ttgttatact atgaaacctt
     7621 taaaagtaat attagatgaa aatgaagatt taaaagaact atataaaagt atgcaataaa
     7681 tgtatgtagg cgatgaatta atgacaatga attttatata gatgaggatt atattttata
     7741 taaaagagtg tcatatcttt aatataggac atgaagtaat aaaagagttt aaaattatat
     7801 ttttaatatg atatatgagt tatcaaatca ctatcattat agctatatta ttataattta
     7861 aatgataatg atagtgattt gataaaaagg ttttaaagat tgtaaaaata cattaagatg
     7921 attttcaaac agcttataga ggcttgtacg gggtcataat tataaactag gaggatataa
     7981 aaatgaaatt caaaaatata gttatgggaa tgttattagg tttaatcatt gtaggagttt
     8041 caggagtaac aaaagttaag gctgctgaat tagataattt ttatatagag tccacagata
     8101 gagttattga agttttagaa gatgaaagta tagttttata tgatactaaa gagcaagttt
     8161 ataatttcta tccaatttgt atgggagact ggaactatag ctttaaaaat aaaaaagatt
     8221 tagatagcgc agttgcaact tacaaagagc tatcaagtaa tataaatcat ttaagtaata
     8281 tatacgtggt taataagtta aatgacaatg gaaacattaa agtattttta aatgatggaa
     8341 gttcaattgt ttatataaaa caggataata aatattactt ttatccttct tgtatgggtg
     8401 actggtactt aacattggat agtaaaattt atttaactaa ttgtattaat acttatatag
     8461 aatattatta ttccttgtca actggaggtg gcacaaatga ataataatga taagttatct
     8521 atattaaatc aattggaata catagaaaat gatattgatg caagaaatga aatgattgaa
     8581 aaatttaagg acttattggt caaatatcca aagagtagag atataagaag atatataaaa
     8641 caacaaactc aatttttaaa tggtattaga tatatatatg ataatttaga tattttgaag
     8701 aatatgataa ataatgacaa gaagtgagaa cattactaga cataacaatg tctactgaat
     8761 gtcaaagaga ttcatttagt aaatacaaag ttattatgtc aatttttaat taaattgtaa
     8821 tttaacataa aaatgtagtt actattttaa aactcataat aactacattt aataatttaa
     8881 aaagggagat ggaaaaagtg aataaattaa aagttaaagg aaaacaaaaa tttatgaatt
     8941 tagaaatacc agttatagaa ggtggttttg gtgaagataa aaaatgtatg acggataaga
     9001 ctatatcaga aatacataat cagcctaatt ttaagataag agagagaata aaggataata
     9061 taaaaagatt taaagaagac atagattata tagatttaaa agttatccgt gacacggatg
     9121 acaatttaga gctattacaa acacttggat atagtaaaat gcaaatatca aaagctgata
     9181 atatattttt attatctgaa agaggatatt caaaattaat taagataatg gatactgatt
     9241 tagcttggga aatacacgat aaaataatgg atgaatattt tagtatgaga aaaaagttaa
     9301 atgacccttt tgaaaactta tctccagagt taaaagttat aatcaaacta gaacaaagac
     9361 aagcagaact tgaatctggt caacatgaat tagaggaaaa agttaatgtc ctatatgatt
     9421 tttctactat agactacaat caacaagaaa ctattagaag tttggtaaat agaatggtaa
     9481 tacaagtata tcaatgttta aatttttcag cttatgaaga tgctaaatta agaaaaagag
     9541 tatacttttc actatggaaa gattatagaa aaaaatttgg tgttaatagc tataagaaca
     9601 ctgctaaaaa gaactatgac aatgcaatta aattcataga aaactgggag cctaattatg
     9661 atttaagcaa tgaaatatac atgtttcaaa aagttgaaaa tatataagaa taatgattgg
     9721 agattattat gatatgttat gtcaaagata tacatactat aagcctatat gatgctttta
     9781 gatggcatac aatggggttg tcattcatta ttaaagatgg taaattaaaa ggttttacaa
     9841 tgtaactaat taattgagaa gagaattatg ataacattct tttctcaatt aattatcatt
     9901 cctaataaat aaagttcaaa agatagtgta aaagataata cattttgtta atattagtaa
     9961 tataaaataa atttaaaaat ggagatgaat atattgattg aaaataagtt tatagaaaaa
    10021 ttaaaatata ccaaaaacat accaaactct ttatacaaag acttaagaca taatttttta
    10081 tttcattcaa ataaaataga aggaagtaca ttcacaagtg aaagtttaca agatttaatt
    10141 gagcacaaca ttgtaaaagg tgaccatact ttagatgatg ttataacaac agtaaattca
    10201 agctatgtat ttgaaactgt aatagataca ttgggcgaat acttaactga aaagtacata
    10261 aaggatttac atggacaact tatgtataat tcaaatattc ataatatggg gctatctggt
    10321 atatacaaaa aaataccaaa tagaataata ggaactaata gtgtattagc ccaaccattt
    10381 gaagtagaac caaagatgtt agaacttatt gagtggtata ataatttaaa agatattaca
    10441 ttaaaagact tagcagaatt tcattataga tttgaaatca ttcatccttt tcaagatggc
    10501 aatggaagaa taggaagatt tatattactt aaacaaatac ttgagaataa tttgaaattg
    10561 attataatat cgagtgatac tgtagatgat tatagacagg ctttaaaagg ttgtcataat
    10621 gaatattata aacctttaga gcaatatttt gaaagtttag atgactacaa agaaaaaaat
    10681 aaagatttat ggagttttta acctaataat taaacaaatg aatggaagtg aaaactatgc
    10741 tttacagaac ggaagattta aatacttttg caggtattgg aagttctaaa ggtgtttatt
    10801 gtcaactgcc actaccctaa agggtagtag cttgtaaaag ctagttgaat agcctaagtt
    10861 tacactacgt tatgtaagaa tatatagcta ctttagaata ctttaaactt aaaggaatat
    10921 tccacatatt aaaatactct ttttacaaat ttataattaa atcataatct aactttttat
    10981 ttgaaaaact ttcaattaac cttgctttat cattagcatt ctttaaaatg tagttattta
    11041 accaataaac tatcgcatca cccctataat ggagtccaat ttggactcca ttttcataac
    11101 accaaaaagg atgctcatct tctgtattgt caatccaaat atgaaattcg tttctaattt
    11161 catatttttt taaactaatt tcattaggaa catatatata atcaggacta tagttgccac
    11221 caaaatttac ttttataacc ataattatag tctccttttt aaataattat aacatatttt
    11281 ataaaaaact ggtaattaca cttgtagttt aattccaaaa tatagcattc tacaagtgta
    11341 tctaaattta ttaaagcaa
//
LOCUS       DAMJVK010000111        11250 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.116, whole genome shotgun sequence.
ACCESSION   DAMJVK010000111 DAMJVK010000000
VERSION     DAMJVK010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11250
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.116"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            232..927
                     /gene="cas6"
                     /locus_tag="PC414_003195"
     CDS             232..927
                     /gene="cas6"
                     /locus_tag="PC414_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HDF2465572.1"
                     /translation="MIKCLEMSVKVYLLENISLDNTSKEIRKLIDDTLIIEEKYKEFH
                     NKAGFKNYSFNLLYPLEKDKVYKKDCIYTFIIRTVDSELGMYFMNNLKNQHSDKIKVI
                     TMSKKVIPKKHIESIYSVTPIIAKFEDGYWRSNKTVEDLEKRIRENLIKKYNEFTNSK
                     IDEDFELFQVIKFINRKPISTKYKNISLLGDKISFKVSENDMAQEIANFALGVGVGEI
                     NSRGFGFVNYRWL"
     gene            918..2678
                     /locus_tag="PC414_003196"
     CDS             918..2678
                     /locus_tag="PC414_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cse4"
                     /protein_id="HDF2465573.1"
                     /translation="MVIGGENLLKDCLEIFEKEYKSLGNRLITDSYVLSYGTYILVKN
                     FSNIDILEVSKNNVDSTEDIYHKFCELDYLSKLVSIDKSVDSKKIIHSNNYLSFFVKK
                     ENLRPDKNNNVKLTEEIIDNYYDALLNPRIKYKSDKRKKEIYDSIENKYGKVDENKLN
                     KIRLWIKTNIFDLVESVKKDKNYLKIFFDEKIELYNDENQKYLYPNIYNKNDFNVIID
                     GKNYGLPNDNMQLNEKKPFLKLKTRIRNSTPYLISDDEVLLQKAFFDYLTNKAANGEE
                     VIYISSEKGIHRKTDIPNEFSGYILRIKKGKELEIHDFDTVVNLNTKIKRINVECIIP
                     IEYERHNLKPNLEIGYINNLIKLEKTIDTVFFNKFMCNSYFKEIKNINLSDLVLRDIL
                     IRHREGLYTWFYKGRDSIVKQTFSNYSMGIIENSIKNNNYIRAKEQYNLRIAIINYFH
                     GGSNMADKIKVISESLFEKINSIKTKCIQSDEEYYFAVGQLASYFISKNKSKNKNHSL
                     INPILNAQNRKKIMLEIEKLFKKYNYDISYDNNRRFKNLYSMIVGYEVEEKKVNRDLL
                     IGGYLYYNLIYTKSEENENE"
     gene            2665..3660
                     /locus_tag="PC414_003197"
     CDS             2665..3660
                     /locus_tag="PC414_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I CRISPR-associated protein Cas7"
                     /protein_id="HDF2465574.1"
                     /translation="MKMNRRVYGVLGIKSIMSNWNADFSGYPKTTSTGEVFGSDKALK
                     FPMKKMWENEGEKVLYVKSLKIDESKKGEIKIRPKSLKERYEELFDIEDLKLEKNQQK
                     VLENLFKAIDVKNFGATFAEEGNNIGIMGAVQIGQGFNKYEDINVEEQQILSPFRDPK
                     AKDKKKSTNEEEKEDANSTTLGKKIVSDEAHYFYPFSINPKAYEVFVNMGVTDGYTKN
                     DYKKFKEAALVSATAFNTNSKLGCENEFALFVETDEELYLPDLAQYITFEKREKSKDL
                     ICINIIHLIAELKDDIKSVEIYYNPYKIEIKPFKDEILPKIDKVKFMNIFTRKEV"
     gene            3662..4402
                     /gene="cas5b"
                     /locus_tag="PC414_003198"
     CDS             3662..4402
                     /gene="cas5b"
                     /locus_tag="PC414_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas5"
                     /protein_id="HDF2465575.1"
                     /translation="MEVLKFNLSGKTAFFKKPDVNSIIYFTYGNIHKIALMGILGACL
                     GLGGYNQQSKEDEYPEFYSLLKDIKMSIVPKHEFGYINKKVHIFNNSVGYASKEEGGN
                     LIIKEQWLENVSWDIYIQLEDSTTSRELKRRFLSNKFVYIPYLGKNDHIANISEIEII
                     TDFYKTEDINRVDSFFIREDFEFEKSYFDDNDDYKEPFKYQEKLPYRLHRMTNQYILE
                     TIIFTNMSVKLINKKEVYQVKGLNLIFI"
     gene            4426..6984
                     /locus_tag="PC414_003199"
     CDS             4426..6984
                     /locus_tag="PC414_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HDF2465576.1"
                     /translation="MYFKKVELFDISNNINNCKNIYAHINKDRKPETLKEHSDLSYSY
                     LMKLCVKKDLSSCLERIENKFFKKDSFLSKNFYREIILNMVYMHDIGKINSSFQFVKM
                     QNKNFKKISLRYTKHSFISSMLYFDYYFEKLKKYDFEEKKILSLFLMINTYTISKHHG
                     VLKSFKEFLFLFKDEFINIKENFKDLFKNNYNLELKSVPKTIEKMVRGVEAYLDDSHI
                     KDNLAIDLYIYSKLMFSIVTSCDFYATSEYNSGNSIDDFGTIDNYNKYYDVLKKSEIY
                     KGMKKYREYLNNNSECPYKENDINRLRTEMSIEAEDAITHNTRKRIFYLEAPTGSGKT
                     VTSINLALKSIELNQSLKKIFYIFPFNTLVEQTKDVFINEIFNSVKDIQKDVVVINSI
                     TPIQTEDKEEDNKNVEYCVTKQEIDYDKSLLNRQFLHYPIVLTTNIGFFNYLFGVSRE
                     ECFPLIHMINSVIILDEIQNYKNEIWKEIILFLEKYADILNIKFIIMSATLPRLNKLG
                     CNDDNFAYLVKNREKFFKNHLFKDRVNISFEMLNSEINDIEEISEKVIEEAEIYNKIL
                     IEFIKKSSALKFYKNIKEKLKYKLEDVFLLTGDDNKLERKKIINKIKDMNSVILVATQ
                     VVEAGVDIDMDLGFKDISTIDSDEQFLGRINRSCKKLNSKVYFFNLDDASKIYKKDVR
                     KERHLTLNEESNRKIILDKDFEKYYDKIIARIEENKHKHNDKNIEVFIKDIVGNLNFT
                     EVKKWMALIPDLKEYTIFLNTIVKDESGNMIVGSDVWYEYISLLKNDNVEYSEKRVKM
                     SEIMEKLDYFTYKVQKFDNSFNDLVGDIFYIDDGSKYFTEGKFDRSKFNQNEFL"
     repeat_region   7109..9581
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=7174..7203
                     /rpt_unit_seq="gttaaacagtaacatgagatgtatttaaat"
     gene            9666..10160
                     /locus_tag="PC414_003200"
     CDS             9666..10160
                     /locus_tag="PC414_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HDF2465577.1"
                     /translation="MKITGTLINYYFHCKRQCWLLGNRINLEENSEDVKIGRLLHELK
                     EEKTKYKEIAIENIKIDKLTREYLVEVKKSDADIEAVKWQVLLYLKKLKEKGIVRKGK
                     IEFIEKNKTNKKIVFVDLSEVSEEQLISIEREIVNLINSDKAPCPDLKKGCKKCAYYE
                     YCYI"
     gene            10195..11193
                     /gene="cas1b"
                     /locus_tag="PC414_003201"
     CDS             10195..11193
                     /gene="cas1b"
                     /locus_tag="PC414_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated endonuclease Cas1"
                     /protein_id="HDF2465578.1"
                     /translation="MGKTKYITSMGELTRKDNSICFRKNGRNVYIPVENTSEIFCLNE
                     VSLNSKLLDFLSRANIVVHFFNYYGGYSGTFYPKEYLISGKVTVAQVKTFEDRREEIA
                     KSIVNGIRINAINTLSHYYKHGNSEIQPLLSHLKKDSINKLEKSNNIKEILSVEGDIW
                     QTFYTSFKYILREEFIFNKRVKRPPDNPLNAMISFGNSILYAKTVTAIYNTHLNQSVS
                     FLHEPSESRFSLSLDLSENFKHVITFKTIFDLVNNRKINIEKHFDKNLNYCVLNDEGK
                     QIFINSLEERLESVKEHPKLKRKVSLKTCIKLDAYKMLKCIMEEKSFIPYNIKDGL"
BASE COUNT         4875 a          981 c         1717 g         3677 t
ORIGIN      
        1 gaaatttatt atcaataatc taaatataga aattaaaaat gataaagaga ttgtaattat
       61 atttttttga aactttgtga ttttatttgt cgaataaaaa ataaaaaatt ttaaaggaaa
      121 aagttaaaaa ataaagaatt ttataactat aaattatatt ttagagttta ttgttattaa
      181 atagatgaaa aaatcctaat atggattatc agaaaatgag gaggttgtta attgataaaa
      241 tgtcttgaaa tgtctgtaaa agtgtatctt cttgaaaata tttctttaga taatacatca
      301 aaagaaatac gtaaattaat agatgataca ttaattatag aagaaaaata taaggaattt
      361 cataataaag ctggatttaa gaattattct tttaatttat tatacccatt agaaaaagat
      421 aaggtttata agaaggattg tatatatact tttattatta gaactgtaga tagtgaatta
      481 ggaatgtatt ttatgaataa tttaaaaaat cagcattctg ataaaataaa ggttattaca
      541 atgagtaaaa aagtgatccc taaaaaacat atagaaagta tttactctgt cactccaata
      601 atagctaaat ttgaggatgg gtattggaga agtaataaaa cagtagaaga tttagaaaaa
      661 agaatcagag aaaatttaat aaaaaaatat aatgaattta ctaacagtaa aatagatgaa
      721 gattttgaat tatttcaagt tattaaattt ataaatagga aacctatttc tactaaatat
      781 aagaatatat ctttacttgg tgataaaata agttttaaag tatcagaaaa tgatatggct
      841 caggaaattg caaattttgc acttggagtt ggagtcggag aaattaattc tcgtggattt
      901 ggttttgtaa actatagatg gttataggag gtgaaaactt gttaaaggat tgtttagaga
      961 tttttgaaaa agaatacaaa tctttaggaa atagattgat aactgatagc tatgtcctat
     1021 cctatggaac atatatattg gtgaaaaatt tttcgaatat agatatatta gaagtttcta
     1081 aaaataatgt ggatagtaca gaagatatat accataaatt ttgtgaactg gattatttat
     1141 caaaactggt gtctatagat aaatcagttg attccaaaaa aataatacat agtaataatt
     1201 atctttcatt ttttgtaaaa aaggaaaact taagacctga taaaaataat aatgtaaaac
     1261 ttacagaaga aattattgat aattattatg atgctttgtt aaacccaaga attaaatata
     1321 aatctgataa aagaaaaaaa gaaatctatg attcaataga aaataaatat ggtaaagtgg
     1381 atgaaaataa attaaataaa ataagactat ggataaaaac aaatatattt gatttagttg
     1441 aatctgttaa aaaagataaa aattatttaa aaatattttt tgatgaaaaa atagagttat
     1501 acaatgatga aaaccaaaaa tatttatatc caaatatata taataaaaat gatttcaatg
     1561 taattattga tggtaaaaac tatgggttac caaacgataa tatgcaatta aatgaaaaaa
     1621 agccattttt gaaattaaaa acaagaatta gaaattcaac tccttatttg attagtgatg
     1681 atgaagtttt gttacagaaa gctttttttg attatctaac gaataaagca gcgaatggag
     1741 aagaagtaat atatataagt agcgaaaagg gaattcatag aaaaactgat ataccaaatg
     1801 aatttagtgg ttatatactg agaataaaaa agggtaaaga attagaaata catgattttg
     1861 atactgtggt aaatttaaac actaaaataa aaagaataaa tgtagaatgt ataataccaa
     1921 tagaatatga aagacataat ttaaaaccaa atttagagat aggatatatt aataatctta
     1981 taaaattgga aaaaacgatt gatactgtat tctttaataa atttatgtgc aatagttatt
     2041 ttaaagaaat taaaaatata aacttaagtg atttagttct aagggatata cttattagac
     2101 atagagaagg tttatatacc tggttttata aagggagaga tagtatagta aagcaaactt
     2161 tttcaaacta ttctatgggt ataattgaaa attctataaa aaacaataac tacataagag
     2221 caaaggagca atataattta agaatagcta taataaatta ttttcatgga ggaagtaata
     2281 tggcagataa aataaaagtt attagtgaat ctttatttga aaaaataaat tctataaaaa
     2341 cgaaatgtat acaaagtgat gaagaatatt attttgcagt tgggcaatta gcatcgtatt
     2401 ttatatctaa aaataagtca aaaaataaaa atcattctct gattaatcct atactaaatg
     2461 cacaaaatag aaaaaaaatc atgttagaga tagaaaaatt atttaaaaaa tataattatg
     2521 atataagtta tgacaacaat aggcgattta agaatctata ttctatgatt gtaggatatg
     2581 aggtagaaga aaaaaaagtt aatagagatt tattaattgg tggatatcta tattacaatt
     2641 taatatacac aaaaagtgag gagaatgaaa atgaatagaa gagtatatgg agtattaggt
     2701 ataaaatcta taatgtcaaa ctggaatgct gatttttctg gatatccaaa gacgacatca
     2761 acaggagaag tttttgggag tgataaagca cttaagtttc ccatgaaaaa aatgtgggaa
     2821 aatgaaggtg aaaaagtttt atatgttaaa tcattaaaaa tagatgaatc taagaaagga
     2881 gaaataaaaa ttagaccaaa atcattaaaa gagagatatg aagaattgtt tgatatagag
     2941 gacctaaaac tagaaaagaa tcaacaaaaa gtactggaaa atctatttaa agctattgat
     3001 gttaaaaatt ttggtgcaac atttgcagaa gaaggaaata atataggaat tatgggcgca
     3061 gtacaaattg gacaaggatt taataagtat gaagatatta atgttgaaga acaacaaata
     3121 ttatccccat ttagggatcc aaaagcaaaa gataagaaaa agtcaacaaa tgaagaagaa
     3181 aaagaagatg caaattcaac tacattagga aaaaaaatag taagtgatga ggctcattac
     3241 ttttatccat tttcaataaa tccaaaagct tatgaagttt ttgttaatat gggtgtgact
     3301 gatggatata ctaaaaatga ttataaaaaa tttaaagaag ctgctttagt aagtgcaact
     3361 gcattcaata caaattcaaa gcttggttgc gaaaatgaat ttgcattatt tgttgaaact
     3421 gatgaagagt tatacctacc agatttggca caatatataa cttttgaaaa gagagaaaaa
     3481 tcaaaagatt taatttgtat aaatataatt catcttatag cagaattaaa agatgatata
     3541 aagagcgttg agatatatta taacccatat aaaatagaaa taaagccttt taaagatgaa
     3601 atattaccta agatagataa agttaaattt atgaacatat ttacaagaaa agaggtataa
     3661 tatggaggtt ttgaaattta acttaagtgg aaaaactgct ttttttaaaa aaccagatgt
     3721 aaatagcata atttatttta cgtatggaaa catacataaa attgcactta tgggtatttt
     3781 aggagcttgt ttaggacttg gtggatataa tcaacagagt aaggaagatg agtatccaga
     3841 attctactct ttacttaagg atataaaaat gtcaatagtt ccaaagcatg aatttggata
     3901 tataaataaa aaagtccata tatttaataa ttctgtagga tatgcatcaa aagaagaagg
     3961 tggaaattta ataataaaag aacagtggct tgaaaatgtt agttgggata tatatataca
     4021 attagaagat tcaactacaa gtagagagct taaaagaaga tttttatcta ataaatttgt
     4081 atatatacct tacttaggca aaaacgatca tatagctaat ataagtgaaa tagagataat
     4141 tactgatttt tacaaaacag aagatattaa cagagtggac agtttcttta taagagagga
     4201 ttttgagttt gaaaaatctt attttgatga taatgatgat tataaagagc catttaaata
     4261 tcaggaaaaa cttccttata gactgcaccg aatgactaat caatatattt tggaaactat
     4321 catatttact aatatgtcag ttaaattgat taataaaaaa gaggtatatc aagtaaaggg
     4381 tttaaattta atctttattt aaatttatag gtttaggaga attatatgta ctttaaaaaa
     4441 gttgagttgt ttgatataag taataatata aataattgca aaaatattta tgctcatata
     4501 aataaagaca ggaagccaga aactttaaaa gaacattcag acttatcata ctcatatctt
     4561 atgaaattat gtgtaaaaaa agatttaagc tcttgcctag agagaataga gaataaattt
     4621 tttaaaaaag attctttttt aagtaagaat ttttatagag agattatctt gaatatggtt
     4681 tatatgcatg atataggtaa gattaattct tcatttcagt ttgtaaaaat gcaaaataaa
     4741 aattttaaaa aaatttcact tagatatact aaacattctt ttatctcttc aatgctatat
     4801 tttgattact attttgaaaa gcttaaaaaa tatgattttg aagaaaaaaa aatattgagt
     4861 ttatttttga tgataaatac atatacaata tcaaaacatc atggggttct gaagtcattt
     4921 aaagaatttt tatttttatt taaagatgag tttataaaca ttaaagaaaa ttttaaagat
     4981 ttatttaaaa acaattataa tttagagctt aaatcagttc ccaaaactat agaaaaaatg
     5041 gtaagaggtg tagaagcgta tttagatgat agccatataa aggataactt agctatagac
     5101 ttatatatat attcaaaatt gatgttttca attgttacat cttgtgattt ttatgctaca
     5161 tcagaatata atagtggtaa ttctatagat gattttggga caattgataa ttataataag
     5221 tattatgatg tattaaaaaa atctgaaata tataagggga tgaaaaaata tagggaatac
     5281 ttaaataaca atagtgaatg cccatataaa gagaatgata taaacagatt gagaacagaa
     5341 atgagtatag aagctgaaga tgcaattaca cataatacga gaaaaagaat attttattta
     5401 gaagcaccaa ctggaagtgg aaaaactgtt acatctataa atctagcact gaaatctata
     5461 gagttaaatc aatctttaaa aaagatattt tatatatttc catttaacac attagtagag
     5521 caaacaaaag atgtatttat aaatgaaata tttaattctg ttaaagacat acaaaaagat
     5581 gttgtagtta taaattcaat aacaccaata caaacagaag ataaagaaga agataataaa
     5641 aatgttgaat attgtgttac taaacaagaa attgattatg ataaatcatt gttaaataga
     5701 caatttttac actatcctat agtacttaca actaatatag gtttttttaa ctatttgttt
     5761 ggtgtatcaa gagaagaatg ttttccattg atacatatga taaatagtgt aattatatta
     5821 gatgaaatac aaaattataa aaatgagata tggaaagaaa ttattttgtt tttagaaaaa
     5881 tatgcagata tattaaatat aaaatttatt ataatgtctg caactttacc tagattaaat
     5941 aaattaggat gtaatgatga taattttgca tatctagtaa aaaacagaga aaaatttttt
     6001 aaaaatcacc tttttaaaga tagagtaaac ataagttttg agatgttaaa ttcagaaatt
     6061 aatgatattg aagaaatatc agaaaaggtt atagaagaag cagaaatata taataagata
     6121 ctgatagagt ttataaagaa atcatcagca ctaaaatttt ataaaaatat taaagaaaaa
     6181 ttgaagtata agttagaaga tgttttttta ttaacaggtg atgataataa attagaaaga
     6241 aaaaaaataa taaataaaat taaagatatg aactctgtaa ttttggttgc tacacaagtt
     6301 gtagaggctg gagtagatat agatatggat ttgggattta aagatatatc tacaattgat
     6361 tctgatgaac aatttttggg aagaataaat agatcttgta aaaaattaaa ttcaaaagta
     6421 tactttttta atttagatga tgcaagtaag atatataaga aagatgttag aaaagagaga
     6481 catttaactc ttaatgagga aagtaataga aaaattattt tagataaaga ctttgaaaag
     6541 tattatgata aaataatagc cagaatagaa gaaaataagc ataaacataa tgacaagaat
     6601 atagaggttt ttataaaaga tattgtggga aatttgaatt ttacagaggt aaaaaaatgg
     6661 atggctttaa taccagattt aaaagagtat acaatctttt taaatactat agttaaagat
     6721 gaatctggaa atatgattgt aggaagtgat gtatggtatg aatatatatc actactaaaa
     6781 aatgataatg ttgagtattc tgaaaagagg gtgaaaatgt ctgaaattat ggaaaaacta
     6841 gattatttta cgtataaagt tcagaaattt gataattcat ttaatgattt agttggagat
     6901 attttttata ttgatgatgg aagtaagtat tttacagagg gaaagtttga tagaagtaaa
     6961 tttaatcaaa atgaatttct ataatgtatt tagattatat agagaatatt tatcccaagt
     7021 taattaatta gaattagttt aaaactattg aaatatagat attttatagc ttatatattt
     7081 tttctaggat tagctgggat aaaaaatctg tatattagtg ttttcagtgt atttaagggg
     7141 attatatttt taagaagtac tgattttaaa gtggttaaac agtaacatga gatgtattta
     7201 aatttttact acataattaa atcgtagtca ataccgtaca ggttaaacag taacatgaga
     7261 tgtatttaaa tctctgaata gccaatcaac tttgctgcat tactcttgtt aaacagtaac
     7321 atgagatgta tttaaatgat aatagttata tatatttttt acaatatatt attgttaaac
     7381 agtaacatga gatgtattta aatttacttc tcctgttttg aaatctatat attcatcatg
     7441 ttaaacagta acatgagatg tatttaaata taatattgat acaaatattt tgggtaaatg
     7501 gacttgttaa acagtaacat gagatgtatt taaattcgat atggtaacaa atttataaaa
     7561 gatatagaat gggttaaaca gtaacatgag atgtatttaa attaataatg cacctatttt
     7621 aacagtacct ggtctaaagt taaacagtaa catgagatgt atttaaattc aagtctagga
     7681 gacaacataa atagtacaga tgggttaaac agtaacatga gatgtattta aattagtaaa
     7741 accagatgga actgttgtaa taccaccaaa gttaaacagt aacatgagat gtatttaaat
     7801 ttaaaaacat cttgaggaat atctttaaca gtaattgtta aacagtaaca tgagatgtat
     7861 ttaaatgcat tttcaggtgc atgataaata ggtaaattag atgttaaaca gtaacatgag
     7921 atgtatttaa attttttgta attagccaga ttatcaactc ccaaaatagt taaacagtaa
     7981 catgagatgt atttaaatta aatatcgtcc ttcgtagtta aagcaacacc agaaggttaa
     8041 acagtaacat gagatgtatt taaatcgtaa acaaaattta tcaagagaag aatttctaag
     8101 gttaaacagt aacatgagat gtatttaaat ttatatttga taagagtatc aaatcgactt
     8161 aaaatagtta aacagtaaca tgagatgtat ttaaattcaa tcttaataca cagacatttt
     8221 taaaaacaaa acgttaaaca gtaacatgag atgtatttaa ataaatagac aaatattgaa
     8281 tagctctgtc ttcatcaagt taaacagtaa catgagatgt atttaaatat atttacaaaa
     8341 tctctaacat attttttaac ttctgttaaa cagtaacatg agatgtattt aaattctttt
     8401 aaagagtcct cgggagcctc gaaaacttca gttaaacagt aacatgagat gtatttaaat
     8461 tactcaaata aatttctaat ttgtttatct actaacgtta aacagtaaca tgagatgtat
     8521 ttaaattaga atttacaaca atataagcct ctttaaccat aagttaaaca gtaacatgag
     8581 atgtatttaa atatgttttt agtagtacat ttaataaaac tttttaaagt taaacagtaa
     8641 catgagatgt atttaaatga aggaaccgta ccttgataaa tcacgaccgt acccgttaaa
     8701 cagtaacatg agatgtattt aaattgtata tttgatgttg ttatttctcc tgtgtataat
     8761 gttaaacagt aacatgagat gtatttaaat gaagttccac ccccaccacc cccactactt
     8821 tgagaagtta aacagtaaca tgagatgtat ttaaattaac aatagagtaa atataataaa
     8881 caagcataca aagttaaaca gtaacatgag atgtatttaa atcccctttt gcatggtact
     8941 agacacgcat atatggtggt taaacagtaa catgagatgt atttaaatac gcctatatgc
     9001 ggagtaacag atttttgcct ggatggttaa acagtaacat gagatgtatt taaatactgt
     9061 tttgggatag actggaatta aatggtctgt gttaaacagt aacatgagat gtatttaaat
     9121 ttttggaatg aagcatcaac agataataaa gaaaaagtta aacagtaaca tgagatgtat
     9181 ttaaatcaag gaaacaaaag agaagtgaaa agaaatataa tgttaaacag taacatgaga
     9241 tgtatttaaa taatctttta aatggaaatt aagtaataaa agagagggtt aaacagtaac
     9301 atgagatgta tttaaataac acaatattat ctttaataga attaaacaaa tgagttaaac
     9361 agtaacatga gatgtattta aatttttgtt ggtcttgtgc ttcttttaaa agctgattag
     9421 ttaaacagta acatgagatg tatttaaatt taaaaagttc tgttattaaa acatccaaac
     9481 caatcgttaa acagtaacat gagatgtatt taaattctgt accatctagc caaaccgttt
     9541 ttgaagttaa agttaaacag taacataaga tgtatctaaa ttactctatc ataaaatttt
     9601 aatatattaa acaaactaat atgtgatata cttatgtata aataacaaaa taaataggtg
     9661 atgttatgaa aattacagga actttgataa attactattt ccattgtaaa agacaatgct
     9721 ggttgcttgg taatagaata aatttggaag aaaatagtga agatgtaaaa ataggtagac
     9781 ttcttcatga actaaaagaa gaaaaaacaa aatataaaga aattgcaatt gaaaatataa
     9841 aaatagacaa attaacaaga gaatatttag tagaggttaa aaagtctgat gctgatatag
     9901 aagctgttaa gtggcaagtt cttttatatt taaaaaagtt aaaagaaaag ggaattgtaa
     9961 gaaaagggaa aattgaattt atagaaaaaa ataaaactaa taaaaaaata gtctttgtag
    10021 atttgagtga agtatctgaa gaacagctta tatctataga aagagaaatt gttaatttaa
    10081 taaatagtga taaagcacca tgtcctgatt taaaaaaagg atgtaaaaaa tgtgcctatt
    10141 atgaatattg ttatatataa agaggtataa gaaacatata aggaggtgtt aatattggga
    10201 aagacaaaat atattacctc aatgggtgaa ctcacaagaa aagataattc tatatgtttt
    10261 agaaaaaatg gaaggaatgt atatatacca gtagaaaata ctagtgaaat attctgtcta
    10321 aatgaggtat ctttaaattc aaaattactg gattttttat caagagcaaa tattgttgtt
    10381 cattttttta attactatgg tggatatagt ggaacttttt atcctaaaga atatttaata
    10441 agtggaaaag taacagtggc acaggtaaaa acctttgaag atagaagaga agaaatagca
    10501 aaatccatag tcaatggtat aaggattaat gcaataaata cattatcgca ttactataag
    10561 catggaaatt cagaaataca accattatta tcacatctaa aaaaagattc tataaacaag
    10621 ttagaaaaat caaataatat aaaagaaata ttatcagtcg aaggagatat ttggcaaaca
    10681 ttttatactt cctttaagta tattttaaga gaggaattta tttttaataa aagagttaag
    10741 agaccaccag ataatccctt aaacgcaatg atatcatttg gtaacagtat tttatatgct
    10801 aaaactgtaa ctgctatata taatacacat ctaaatcaaa gtgtaagttt tttacatgaa
    10861 ccaagtgaaa gtagattttc tttaagtcta gatttaagtg aaaattttaa acatgttata
    10921 acttttaaaa ctatatttga tttagttaat aatagaaaaa taaatattga aaaacatttt
    10981 gataaaaatt tgaactactg tgttctaaat gatgaaggaa agcaaatttt tataaattct
    11041 ttagaagaaa gattagaatc agtcaaagaa catccaaaat taaaacgaaa agtaagttta
    11101 aaaacctgta taaaattaga tgcttacaaa atgttaaagt gtataatgga agaaaaaagt
    11161 tttattccat ataacataaa agacggatta taatttaaac ataaagtaag tattttaata
    11221 tagaactttg atatatataa gaatgataat
//
LOCUS       DAMJVK010000112        11124 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.117, whole genome shotgun sequence.
ACCESSION   DAMJVK010000112 DAMJVK010000000
VERSION     DAMJVK010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11124
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.117"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(<1..210)
                     /locus_tag="PC414_003202"
     CDS             complement(<1..210)
                     /locus_tag="PC414_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465579.1"
                     /translation="MQQKCKKKALSMILSISMGLGPVASFNASNTFADTIEESTNAGN
                     AEGGTNAGNAEGDTNAGDAEGDTNAG"
     gene            479..1051
                     /locus_tag="PC414_003203"
     CDS             479..1051
                     /locus_tag="PC414_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HDF2465580.1"
                     /translation="MKKLVKILINIICIITLIFSSLSIYIKLSEYKKADEVYTELREN
                     TINNSKHQELYDKNNDYRFWLKINNVNIDYPVVQGYNNDFYLTHDFYKNYLPLGSIFM
                     DYRNNFENDKSLIVYGHYMKNKTMFGQLENYTDEAFFKENNLVEINYKVQTYTYEIFS
                     VYTADLINRDYLSIHFNSNYEFKDYLNYIT"
     gene            complement(2064..2726)
                     /locus_tag="PC414_003204"
     CDS             complement(2064..2726)
                     /locus_tag="PC414_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2465581.1"
                     /translation="MKMLKVIDIKKIYGKGENEIKAVDGITLEIEPHKFSAIIGQSGS
                     GKSTLLHCMAGLDKPTLGNVFMDNLDLYTLSDEKLSNLRSKEFGFIFQNYNLIPVINV
                     YDNIVLPILIAKGKIDNNYINDLITKLGLESQINKFPNELSGGQQQRVAIARSLANKP
                     SIIFADEPTGNLDSKTTNEVMNLLKYCVNEYKQTLVMITHNDEIAKMADCIITISDGK
                     VI"
     gene            complement(2743..5298)
                     /locus_tag="PC414_003205"
     CDS             complement(2743..5298)
                     /locus_tag="PC414_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2465582.1"
                     /translation="MGIYLKMSLAYLKKNKLRTLLLIIGVMLGVMLIFGPSVIKDSQN
                     ENNVVAIHELYSGYHVEFSDLTLDDAKKLETDEKVSKGNSVQNLGRISDKKGNSFILT
                     SSNEEYLKSRYNKIIEGRLPKNNNEIVLEKKALEAMNLSEGVGSTLNLTIKKNYKDTN
                     GSNQLFSINKEFKIVGVMEKPKGYYDVVWQYEAVTYGNDETNNIIPHDAISYNSILSF
                     KSGWRNIEGECDYLMKKYKLGQKSFLPNVPLVRELRYVEDDKNDPNVFKRNVLITTVS
                     IIFIFNIFNITLNQSVKEMGLLRLMGAKKRNIRCMIIYQALIITIIGILLGLVAGVTY
                     SYIGISNYNEVLYSEAGLNPKLYINNEIVIKAILVGMISVFISCIIPTFKIGKISPIE
                     SIINTDKIKNHRSRLGIDRLVSKIFGFYGFMGLKNIGRNKVRAAISMLSIALGGYIFI
                     TTFSSMQEEVNSKIEDMQNRYNITMQFGVNADPQISKYTDSDVENIEKIKGVKSIHKI
                     QITNGMFTFNNKDINKEFTTYNGVEKKEIMEDKLVLKLYDDDYIDEKLNDFVEEGSLD
                     DIKKDTDGYPNVAVYNYFYDIVDDHTIKPIFKELNIGDIITVKIPVEENNTIVYKEMK
                     VRVAVILKDEWIAMGDGDFMPDLEIITSNIHSKNITGEQKYTQLGINLENPYDEIVNN
                     EIQKISRSIPGSLFNSRLSYHEMSESIYKSYIKSQIAIIALVLIIASINIFCTIRTNL
                     LIRKKEISTLRALGVSLKNMKKMLIYEALAYAVFSFIIAMIPSFINLVKFTNWNNDAY
                     INYGIENFMSFTFPIKESLLFFAISVFVCLISVVVSNRDFESMNIIEGIKENE"
     gene            complement(5538..5726)
                     /locus_tag="PC414_003206"
     CDS             complement(5538..5726)
                     /locus_tag="PC414_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HDF2465583.1"
                     /translation="MRKYKAILLTLIISIIAIIGFIIYNNYFRKWNEAEKYIDKYMTY
                     QGVSGDDIERITKEKYTK"
     gene            complement(5738..5917)
                     /locus_tag="PC414_003207"
     CDS             complement(5738..5917)
                     /locus_tag="PC414_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465584.1"
                     /translation="MIHIEISEKEFFRKIEEVFDKGEVNVEVDNLGDIKIQVKKFDDK
                     TKSLELKLIKEFKLK"
     gene            complement(5922..6179)
                     /locus_tag="PC414_003208"
     CDS             complement(5922..6179)
                     /locus_tag="PC414_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465585.1"
                     /translation="MSKKISESIENSGLRVAENKSDGSFVLSLGNLEYTKEQPSQVLH
                     YSITKEKKDTKEILSIQCVKDFSYNEKLSEDDKFIMAIYNI"
     gene            complement(6152..6292)
                     /locus_tag="PC414_003209"
     CDS             complement(6152..6292)
                     /locus_tag="PC414_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465586.1"
                     /translation="MVIAISLTLGLITGCTNKVSGTNEKIYTIEQISSIKKTCQRKYQ
                     NQ"
     gene            complement(6439..7832)
                     /locus_tag="PC414_003210"
                     /pseudo
     CDS             complement(6439..7832)
                     /locus_tag="PC414_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428529.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            complement(7826..8501)
                     /locus_tag="PC414_003211"
                     /pseudo
     CDS             complement(7826..8501)
                     /locus_tag="PC414_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438019.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(9049..10578)
                     /locus_tag="PC414_003212"
     CDS             complement(9049..10578)
                     /locus_tag="PC414_003212"
                     /inference="COORDINATES: protein motif:HMM:NF012862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HDF2465587.1"
                     /translation="MKSLKALIVGVSKYSIVKNKDLLFCKNDIIEINNVLHKRLGITS
                     NNIRTLGMEYEVRKDLFFKTFQEISDSIGDDDTFIFYFSGHGCICNNGVHYLVLSDEL
                     VSIQFVIDFIEGLNCKNKIVFIDCCFAGKFNVSDKQKINIKDIFSLFDRKGCAVLVSS
                     SEYQESFSLPDSSISRFTSLLSEALRNEYIVRKGELSLRAISKYINLRMSILNKNYIN
                     CKQNPIFRSNILGDIYFNVKDYKSYIKKKVFQEHDDYIIYNVKSLHISIIKRYSVEVI
                     LKNKFSYCDISEISKEVFDIVKSVEVYNSEIGEDRLKGKDANIIWVYFGKDEIDMING
                     IYMCKTTWIDKYQEKDIWYSVNGKNKFFINGVHTETYDNYESLKSYISQNTITKEKAV
                     YEVKEILTNMINISEEIISFYNEYENKKICEDNIFQIIDSRKKKMDECYNKVINLDFP
                     PIEIKDWFDKCINLVSTIQNFGVYWDHSRTEENRKQCMEMNIEDYYKDLREVIRLSEE
                     I"
BASE COUNT         3720 a         1646 c         1084 g         4674 t
ORIGIN      
        1 tcctgcatta gtatctcctt ctgcatctcc tgcattagta tctccttctg catttcctgc
       61 attagtacct ccttctgcat ttcctgcatt agtactttct tctatagtat ctgcgaaagt
      121 atttgacgca ttaaatgatg ctactggtcc aagtcccatt gagatactta aaatcattga
      181 tagtgctttc tttttacatt tttgttgcat aaactccccc tccccttaat aatttttaat
      241 atacactgta tacatcctta tatacactct aataattctt aatatacgct gtatacattc
      301 taatgcacac tctaataatt tttaatatat acttaaaact tatatttgtc aatgaatata
      361 ctgttttttc aaccttatat attataagta ttcattattt gctaaataac tacaataaaa
      421 attttaatta tataaaattt atttaatttt ttatataata atactaaggg gtaattaatt
      481 gaaaaaacta gtaaaaatat taataaatat tatttgcatt attacgctaa ttttctctag
      541 cctttcgatt tacataaaat tatctgaata taaaaaagct gatgaagtat atacggaact
      601 cagagaaaac actatcaata atagcaaaca tcaagaactt tatgataaaa ataatgatta
      661 tagattctgg cttaaaataa acaacgtaaa tatagactat cctgtagtac aaggatataa
      721 caatgatttc tacctaactc atgattttta taaaaattat cttcctttag gtagtatttt
      781 catggattat agaaataatt ttgaaaatga taagagttta atagtctatg gtcattatat
      841 gaaaaataaa actatgtttg gtcagctaga aaactatact gatgaagcat ttttcaaaga
      901 aaataactta gtagaaataa attataaagt acaaacttat acttatgaaa ttttttcagt
      961 atatactgct gacttaatta acagagatta tttaagtata cactttaata gtaattatga
     1021 gtttaaagat tatttgaatt atataaccta aaaatcagta cacaaatctg atataaaggt
     1081 agatatttca gataaaatta taactttgta tacttgtaac tatgaattta aagatgctcg
     1141 tactatagtt catgctaaat taatttcaaa aaaagagact gataagtagg tttgtgtaaa
     1201 tgcagactta cctacagtct tttttgaacg taaaattagt aagtatggaa taatattcat
     1261 attaaacttt tgtaggcaaa gaaatgaaaa atcaaagaga aataattatt acagatttag
     1321 attaccaagc tgaattaaga aagtgtaaaa ctatggagga tgtagttggt gaagatagta
     1381 tataatagtt taacttataa attatcttgg tgatattaat gaagtttcaa agtatatatt
     1441 atttattgat ataaataata ttaaatctgt tgctaacaga tgtacctttg tttggagaca
     1501 aactatttaa gaaatataac taaaatgttg aagtagctac ctgactttat tttgaaatgc
     1561 gaacaagatt ttggcattaa gataatttag aaagaatcac tggtacacaa ggatgtgaac
     1621 ttagaatgaa tagaagtatt caggcataag gagctttcac ccaaataaaa catagtatga
     1681 actttaaaag aattctaatc agaggcaatc aaaatgttct ttcatagttt atacctgtag
     1741 tgttagacat agtatttata agtttcacaa ataagataat cattaataaa tgtagtatgt
     1801 atattcatcc tctcaaaaat attttttaat cttacacttt gtaaattatt ctattttaaa
     1861 atggtatttt tataccttta tttcttatga ccgttttcaa gctcaaattt tgcataaata
     1921 taaattaatg aaaagcaagt aaaaaaagaa ctgtcgcaca gcaatttaaa attactcatt
     1981 cgacagtcct tatttcgtgg atttaaatag tggagaagaa tcacaagaat aatacccacg
     2041 attaaacctt tatacaatac attttatatg actttaccat cactaatagt tattatacaa
     2101 tcagccatct tagctatttc atcattatga gttatcataa ccaacgtctg cttgtattca
     2161 tttacacaat actttagtaa attcatcacc tcattagttg ttttactgtc taaatttcca
     2221 gtaggttcat ctgcaaaaat tattgatggc ttattggcaa gtgatcttgc tattgcaact
     2281 ctttgttgtt gaccacctga taattcatta ggaaatttat taatctgact ttctaatcct
     2341 aacttagtta ttaaatcatt aatataatta ttatctatct ttcctttagc aattaaaata
     2401 ggaagaacta tattatcata aacattgata acaggtatta gattatagtt ttggaatata
     2461 aacccaaatt ctttacttct aagattagat agcttttcat cacttaaagt atataaatct
     2521 aaattatcca taaatacatt acctaaagtt ggtttatcaa gccctgccat acagtgaagt
     2581 aatgtgcttt ttccagaacc actttgtcca attattgcac taaatttatg gggttctatc
     2641 tctaaagtaa ttccatcaac agcctttatt tcattttctc cttttccata tattttttta
     2701 atatctataa cttttaacat cttcatacat ttacctccca aattattcat tctcttttat
     2761 accttcaata atattcatac tttcaaaatc tctattactt acaactactg atattaaaca
     2821 tacaaataca gatatagcaa agaatagtaa agattcctta attgggaatg taaaagacat
     2881 aaaattctct attccatagt ttatatacgc atcattattc caatttgtaa atttaactag
     2941 atttataaat gatggaatca tagctattat aaagctaaaa actgcatatg ctaatgcttc
     3001 atatataagc atttttttca tatttttaag actaacacct aatgctctaa gcgttgaaat
     3061 ttcttttttt ctaattagta gatttgttct tatagtacag aaaatattaa ttgatgctat
     3121 tattaaaact agtgcaatta ttgctatttg tgatttaatg taacttttat atatactttc
     3181 tgacatttca tgataactta atctactatt aaatagagac cctggtatac ttcttgaaat
     3241 tttctgtatc tcattattaa caatctcatc atatggattt tctaaattta tgcctaattg
     3301 agtgtatttt tgttctcctg ttatgttttt agagtgaata ttacttgtta taatttctag
     3361 atctggcata aaatcgccat ctcccatagc aatccattca tcctttaaaa ttacagccac
     3421 tcttaccttc atctctttat aaacaattgt attattttct tcaacaggaa tttttacagt
     3481 tattatgtct cctatattta attctttgaa tataggcttt atagtatgat catcaactat
     3541 atcataaaaa taattatata ctgctacatt tggatatcca tcagtgtcct ttttaatatc
     3601 atctaagcta ccttcttcaa caaagtcatt taacttttca tctatataat catcatcata
     3661 aagctttaaa actaatttat cttccattat ctcttttttt tctactccat tataggttgt
     3721 aaattctttg tttatatcct tattattaaa agtaaacatt ccatttgtta tttgaatttt
     3781 atgtatactt ttaacaccct taattttttc tatattctct acatcactat ctgtgtattt
     3841 tgatatttgc gggtctgcat ttactccaaa ttgcattgtt atattatatc tattttgcat
     3901 atcttcaatt ttactgttga cctcttcttg catacttgag aatgtagtaa tgaatatata
     3961 tcctcctaaa gcaatagaca acatagatat agcagctcta accttatttc ttccgatatt
     4021 tttaagtccc ataaatccat aaaatccaaa tattttgctt actagtctgt ctattccaag
     4081 tctacttctg tgatttttaa ttttgtctgt atttattatt gattcaatag gtgatatctt
     4141 gccgatttta aaagttggta taatacaaga tataaaaaca gaaatcattc caaccaatat
     4201 agcttttata actatttcat tatttatata taatttaggg ttaagaccag cttcactgta
     4261 taaaacttcg ttataattac taattcctat atatgaatat gtaaccccag ctactaaccc
     4321 aagtagtata cctattattg ttataattaa tgcttgatag attatcatac accttatgtt
     4381 tcttttcttt gcgcccatca gtctaagcag tcccatttct tttactgatt gatttagtgt
     4441 aatattgaaa atattaaata tgaaaattat agatactgta gttattaaaa catttctttt
     4501 aaaaacattc ggatcattct tatcatcttc tacataacga agttccctta caagaggtac
     4561 atttggtaga aaactttttt gtcccaactt atattttttc ataagataat cacattcacc
     4621 ttcaatattt ctccatccac ttttaaaact taatatacta ttataactaa tcgcatcatg
     4681 aggtataatg ttatttgttt catcattgcc ataagttact gcttcgtatt gccaaaccac
     4741 atcatagtac ccttttggtt tttccataac tcctactatt ttaaattctt tatttataga
     4801 aaataattga ttacttccgt tagtatcttt atagttcttt tttattgtta aatttaatgt
     4861 agatcctact ccttcactta aattcattgc ttctagtgct tttttctcta atactatttc
     4921 attgttgttt ttaggtagtc taccttctat tattttgttg tatctacttt ttagatactc
     4981 ttcgtttgat gaagttaata taaatgagtt tcctttttta tctgaaatac gtcctagatt
     5041 ttgtaccgaa ttaccctttg atactttttc atcagtttct aattttttag catcatcaag
     5101 tgtcaaatca ctaaactcta catggtatcc actgtaaagt tcatgtattg ctactacatt
     5161 gttttcattt tgtgaatctt ttataacact aggtccaaat atcaacataa cacctaacat
     5221 aactcctatt attaaaagta atgttctaag tttatttttc tttaaatatg ctagtgacat
     5281 ttttaagtaa atccccatat aatcctccta ttttactata aattatttat ccaactcatc
     5341 tgttaagtag attggtggat attttaaatc ttttaattct tcattatttt ttgaagctta
     5401 ctaccatctt cctcatgata aacttgcagt atcaccttat ttacatgtag tgcataataa
     5461 tcaccattat aaaaatatta gtatttataa cctggataat ttttgtaaaa tgatttataa
     5521 agtatcccat cctaattcta ttttgtatac ttttcttttg taatacgctc tatatcatca
     5581 cctgaaactc cttgatatgt catatattta tctatatatt tttcagcttc attccatttt
     5641 ctaaaatagt tgttgtatat aataaatcct attatggcta taatgcttat aattaaagtt
     5701 aataaaatag ctttgtattt tctcatagtg aatcttccta cttcaattta aattctttaa
     5761 taagttttaa ttctaaagac ttagttttat catcaaattt cttaacttga atttttatat
     5821 ctcccaaatt atcaacctct acatttactt cacctttatc aaatacttct tcaatttttc
     5881 taaaaaattc tttctcactt atttctatat gaatcaatga tctatatgtt ataaatggcc
     5941 attatgaatt tatcatcttc tgacagcttt tcattataag aaaaatcttt aacacattga
     6001 attgataata tttccttcgt atccttcttt tccttagtta ttgaataatg taaaacttgg
     6061 ctaggttgtt cttttgtata ctccaagttt cccaaagaca aaacaaaact tccatctgat
     6121 ttattttcag ctaccctaag accactattt tctattgatt ctgatatttt ctttgacaag
     6181 tcttcttgat actagaaatc tgttcaatag tatatatttt ttcatttgta cctgaaacct
     6241 tatttgtgca tcctgttatt aaacctagtg ttaagcttat agctataacc atagttattg
     6301 taattttttt atcttcatat aatcctcctt atatattgaa aactcgttat ttctcataaa
     6361 aaaaagacgc tatctttttc tattaaaaga taacatccct atatgtttag tatcaatata
     6421 tgttccccaa ctggtaggtt aatcgaccca actatcaaaa ttttgattta atggtatata
     6481 aatattcaat ataaatttgt cttttttatc ttcaaatgat aaatctcctc tatatttttt
     6541 tatagaagat ttgatgcttt aaatccctat tccatgtaca agtttatctt ttttatctgt
     6601 taataataaa cttttattct ttctaattga atttatttta ctattctcac atttaattac
     6661 gaaataggac tttgagatag ttccttttat ttgtatatat ttatcaatac ttttatcatg
     6721 tatcttatcg caagcttcta ttgcattatc taagtatttg caaatatatt agatacatct
     6781 attggtttta taaaattcac tttggaaaaa ttgatatcac aaatgaattt aatttttctt
     6841 ttttcactta catcactttt ttcatttatt attatatcca atataacatt acctgtattg
     6901 taaattgatt taaattcttt aatttaatct tttaaactat ttacataccc atcaattttc
     6961 cttttttata tttgagattt tctatacaat atatatgatt atttatatca tgataaagct
     7021 tcttaacttt agcgtgtgat tcttgaatac ttagataatg agaatactgc atatctaact
     7081 tattttttat tacttcattt tgcaatttta acttattttc cttaattatt tttataaaca
     7141 ctataataat gactaagcta ctagctagta ttaacctagg tatcacaata tacccaacta
     7201 tatatatttc ctaataccca ccaaatcgat acatttttct atttatataa attattaata
     7261 tagtaaataa atttgttatt aaagcaacag ataagtatat atagtttatt ttattatctt
     7321 tatctagtcc caatctttta ttaataaatt taaatatagc acaagctact atataactat
     7381 tattataaga taatagtata tatctacttt agcattatca taactaaaca ctaatagagc
     7441 ttcctctgat gatattacat tacttgtaac tacaaaaaat aaatttatag atataacatc
     7501 ttttattaaa gggtgatata cgtcaaataa gaaaaatgaa actatatatt taacaattcc
     7561 tatcttataa aaataattat agtaagttcc caagtaataa atatcaaata atagattata
     7621 tcatatagat ttttcttcat attataatca tgggtcttat caacattaaa taatataatg
     7681 atcttagtta taagataagc taataataca gtaaaaaaag atattttctt agtcttatct
     7741 gtatctttat cgtaagttaa tatacttttt agtatcatta ttggtgtaaa caatgataat
     7801 attaataata ttttatttat atggtttaac atattctatc accaataaat tttagaaacc
     7861 cctcttttat atctttatat ctatatctac taataggtac ttcttcttga ctgtctaata
     7921 ttgcagtatt tgactttata ttttctacat agtttaagtt agttatataa cttttatgac
     7981 atcttacaag ttcctctata tttaaatctt tttctatttt ttctaggata catatagcat
     8041 caagagtttt attttttgta tgtattagca tgttcttttt ttgaacttct atatattttt
     8101 ttgctagatt agattttgta tgtgttagat ttatttttat ttttttcaat ttttttggta
     8161 catgttaata tgtgtttttc tagttcttga aaatttattg gctttaataa gtatcaataa
     8221 gctcttactt catatctttc ctgtacataa tttattagag atgttataaa tattattatc
     8281 tacttttctt atgtttcttg cgatatctat actatttagt tctgccattt ttatatctaa
     8341 gagtagtgta tcctctcaat taactttgta atgattcttc ttttgagcta tattttttta
     8401 attcgatatt ctttgttttt attttttaat atattatcaa cttgattttt tattacgatt
     8461 tgtatttctt tttcatcatc acaagctgtt attgtaatga taacctctcc taatattgta
     8521 tttttattta gattcttcac tttaggttaa aactttttaa atgtatttat caataatatt
     8581 taaattaaaa atgtataaaa atacctatat ccaattggat ataggtattc gtggatttca
     8641 atggtggaga cgaggggagt ctataagtgg ttatttgatt gttttgttgt tttttgcaag
     8701 tgtaggtata tagctattta gttatgatgt taaactgatt ttttcggata ttttttattg
     8761 tttatttttg tagttggggt attattgggg gggggtggat gatataatct tttaatttta
     8821 taaaagttcc attaacagca tattatacgt ttttaaatat ttactagact ttttcaaaat
     8881 aaatatctaa ttatttaaat caataccaat ataatttata aatggattta taattgtatc
     8941 ttctatcatg tttagtgtaa acaacttatt ttaatttaaa ttcgtaacca acaaagtacc
     9001 aatctgactt ataattctat attagtacaa ctatggaaaa atttaattct atatctcctc
     9061 acttaatctt atgacttctc ttaaatcttt gtaataatcc tctatattca tttccataca
     9121 ttgctttcta ttttcctcag ttctactatg atcccaataa actccaaaat tttgtatagt
     9181 tgacactaag tttatacatt tatcaaacca atctttaatt tcaattggtg gaaaatctaa
     9241 atttataact ttattgtaac actcatccat ttttttcttt ctactatcta taatttgaaa
     9301 aatattatct tcacatattt tcttattttc atattcatta tagaaggata taatttcttc
     9361 agatatgttt atcatatttg ttaaaatttc tttaacttcg tatactgcct tttctttagt
     9421 gattgtattt tgacttatat atgattttaa gctttcgtag ttatcatatg tttcagtgtg
     9481 aacaccgttg ataaaaaatt tatttttccc atttacacta taccatatat ctttctcttg
     9541 atatttatca atccaagtag ttttacacat atatattcca tttatcatat caatttcatc
     9601 tttgccaaaa tatacccata ttatattagc atcttttcct tttaatctat cttctcctat
     9661 ttcactatta taaacttcaa cagacttaac tatgtcaaaa acctccttag atatttctga
     9721 tatatcacaa taggagaatt tattttttaa tataacttct actgaatatc tttttattat
     9781 acttatatgt aaagatttga cattgtatat tatgtaatcg tcatgctctt gaaaaacttt
     9841 cttttttata taagatttat aatcttttac attaaaatat atatctccca gtatgttaga
     9901 cctaaagata ggattttgtt tacagtttat ataatttttg ttcaatatac tcatacgcaa
     9961 attaatatac ttgcttattg ctcttaaaga taactcccct tttcttacta tgtattcatt
    10021 tcttaacgct tctgataata aacttgtaaa tctactaatt gaactatctg gaagagaaaa
    10081 tgattcttga tattcgctac ttgatactaa cactgcacat ccttttctat caaacaagga
    10141 aaatatgtct ttaatattta ttttttgttt gtcacttaca ttaaatttac ctgcaaagca
    10201 acaatcaata aatacaattt tatttttaca atttaatcct tctataaaat caattacaaa
    10261 ttgaatagaa actaattcat cacttaatac taagtaatgc actccgttat tacatatgca
    10321 tccatgccct gaaaagtaaa atataaatgt atcatcatct cctatagaat cgcttatttc
    10381 ttgaaaagtt ttaaaaaata aatcttttct gacttcatac tccataccta aagttcttat
    10441 attattcgaa gtaatcccca gtcttttatg taacacatta ttaatttcaa ttatatcatt
    10501 tttacaaaat agtaaatctt tatttttaac tatactatat ttacttactc ctacaattaa
    10561 tgcttttaag cttttcatat tccattcccc ctttataatt tttcaattta atatacttaa
    10621 aatgatgttt taattgtttt attgatttta taaaagctag tatcacacta cttttttgac
    10681 tttttcatta tttacttttg tagttggatt ataatttttc aaggtaagta tatatatatt
    10741 tatttacatc gcacccatat aatttatatc tctgatttat ctgacctgaa cttagtgtaa
    10801 tttaaaccaa tgtaaaatca tctatctctt caccatatat tttttaaaat tccccaaaat
    10861 attgatgtta atagaatttc atgtgttttt tgtattctac tatccataag aattttaaag
    10921 tgagtaaata tttccaatca tttatatctt atttaatatc attttattcc ctcaagtatt
    10981 tatttaaatt gttatgcttt atttataaaa ttatacatac atttctaaag ttagacatac
    11041 atctttaaaa acctttaatt tgtgctactt tttttctaaa aataataaat atataaaaat
    11101 tatatataca aaaaattaag ttat
//
LOCUS       DAMJVK010000113        11019 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.118, whole genome shotgun sequence.
ACCESSION   DAMJVK010000113 DAMJVK010000000
VERSION     DAMJVK010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11019
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.118"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(<1..628)
                     /locus_tag="PC414_003213"
     CDS             complement(<1..628)
                     /locus_tag="PC414_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HDF2465588.1"
                     /translation="MTIPSSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVPPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKL"
     gene            complement(700..1053)
                     /locus_tag="PC414_003214"
     CDS             complement(700..1053)
                     /locus_tag="PC414_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase DNA-binding domain-containing protein"
                     /protein_id="HDF2465589.1"
                     /translation="MSTEKRRDNKDRILRTGESQRKDGRYAYKYVYSFGKPQFVYSWK
                     LVPTDKPPKGKRDDISLRDKEKAIQNDLDDSIDTIGKKMTVCQLYAKKNGLRKNIRLN
                     TKKGRHYLMKILNKE"
     gene            complement(1131..1334)
                     /locus_tag="PC414_003215"
     CDS             complement(1131..1334)
                     /locus_tag="PC414_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HDF2465590.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKRADENLDSGWVI
                     MNSNRIQIKRKQFEKIVDTLDVI"
     gene            complement(1724..1954)
                     /locus_tag="PC414_003216"
     CDS             complement(1724..1954)
                     /locus_tag="PC414_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2465591.1"
                     /translation="MKPTKEVLPFTIILLAVHGDVNAINVILKHFEHYIIRLSQKTLF
                     DEYGNPYIHVEEETKRMLGTKLITAILRFNLS"
     gene            complement(1956..2363)
                     /locus_tag="PC414_003217"
     CDS             complement(1956..2363)
                     /locus_tag="PC414_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2465592.1"
                     /translation="MVDEKTIEHQFDSFCKTVLRNYARDIYDENKRRNDYLVSLESLS
                     LAELSKLSILDDYDSNYICMVSYDYNIRIEDVLMAQAIGKLTKRKQDIILLSFFFNMT
                     NADIATLMDLAENTIHYHKTNALKELKELMQEH"
     gene            complement(2723..3172)
                     /locus_tag="PC414_003218"
                     /pseudo
     CDS             complement(2723..3172)
                     /locus_tag="PC414_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729090.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(3186..4112)
                     /locus_tag="PC414_003219"
     CDS             complement(3186..4112)
                     /locus_tag="PC414_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465593.1"
                     /translation="MSDILSRITNKIGDKNIVDKLSALSKSDLNSLLLEVFDRQANTL
                     TATDILKSYQLNRFTIPSGIDPEEIHALESKLLRKAFNMDIKTIMLSPSAPLGSCSVF
                     GSVDQYNVVSALRGTETLADPSNMLAIIIADKLKRKEENNTIPIHMATTARVIRAQNF
                     VGKGLYSHFGLYCMVSSGIDTGSYTCEKMLLEKHLNYYKDILSEIENVRFSIILRKRN
                     GYKDNDGFFDRMVETIKLIFPNIELSIHNDDVDNNYYKGINFKLSVKQGNETVEIVDG
                     GFVDWTYQMLGNKRERCLISGIGLERFLVAVS"
     gene            complement(4237..5019)
                     /locus_tag="PC414_003220"
     CDS             complement(4237..5019)
                     /locus_tag="PC414_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HDF2465594.1"
                     /translation="MTKTQQKMIQNVLEFSKRDSRIIGLAISSSYITKSLDEYSDLDF
                     LIVADDKSYEEVIEERLLIVEQFGTLVSAFTGEHVGEPRLIISLYDSPVLHVDFKFTT
                     LDGLLDRVEDSAILYEENNCITEIYSKKEAYFPTPDLQWIEDRFWIWVHYACTKIGRR
                     ELFETIDFLSFLRQSVLAPLIQLLLGKLPRGVRKIELDAPQYIEALEKTVANHEYKSC
                     VNALQATINLYLELREELSQSPIVRKVEAERVALQYFQDLIK"
     gene            complement(5175..5795)
                     /locus_tag="PC414_003221"
     CDS             complement(5175..5795)
                     /locus_tag="PC414_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDF2465595.1"
                     /translation="MHLIDFMNEDIKKKLVKKTYKKKETILFAEQENEYVILMIDGIA
                     EAFILNVKGNISTIHLYRSGSFFGEMEQFNDGKKPVEIVAFTDCNVYKLHRDDFLNWL
                     RGDFEATKFLIREISSKLVVNAELIEELSSLSVKERLLRCISLHYYRSELNLLTKKQL
                     ASEVNAPIRSINRAINECVQEHLIAYENKRFSVINQREVLKNLPTT"
     gene            complement(5883..6581)
                     /locus_tag="PC414_003222"
     CDS             complement(5883..6581)
                     /locus_tag="PC414_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2465596.1"
                     /translation="MYKLSYVVRVSTKNLDELKRRCDEVKDFYDDLSIKLVRPFGDML
                     GLHGEFIPVSKRYINDYIQYVTSDFLAGLGFGATQTLGEHEDIYLGYNLDTGKNVYLK
                     PALASQGVKGSITNALASAFIGSLGGGKLFSNNMLVYYAVLFGGQAVIVDPKAERGKW
                     KETLPEIAHEINIVNLTSNDENKGLLDPYVILSRKKDSESLAIDILTFLTGISSRDSD
                     KFLQKNDNWKIIME"
     gene            6682..7497
                     /locus_tag="PC414_003223"
                     /pseudo
     CDS             6682..7497
                     /locus_tag="PC414_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897377.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            8169..8936
                     /locus_tag="PC414_003224"
     CDS             8169..8936
                     /locus_tag="PC414_003224"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDF2465597.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKKDIIKEAKELKDACLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYIHDIPLLFTFRSVVEGGEKLISRDYYTTLN
                     KEISNTGLVDLIDVELFMGDEVIDEVVNFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELGADLPKIAVMPQNEKDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELNSVLNLLHKSIN"
     gene            complement(9264..9941)
                     /locus_tag="PC414_003225"
     CDS             complement(9264..9941)
                     /locus_tag="PC414_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465598.1"
                     /translation="MIVIEGSDKFKIAKEYIDVEYTLFSKVTFRYEKLKFKDNAELEK
                     IKMFKYKNGYIPNKLNLSFGYGFSSYKKQIIRETVDTLRLTEIFSSENIEDIKFIKDG
                     TKKLEISIEKVVKFKRRKKRNYVCCYCPDMYRDIKLDKESINKIYNRKIKIEREVNIF
                     EDEDVIINKKVLKFPKSWTKNMQKYWLSENKYPIHSTVIDDDRYKCCNVQYTKNRVII
                     LYYIYNH"
     gene            complement(10091..10408)
                     /locus_tag="PC414_003226"
     CDS             complement(10091..10408)
                     /locus_tag="PC414_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2465599.1"
                     /translation="MNYIGKRLKEERKKANLTSKEFANMVGVSPWYITQIESGKKNPS
                     LKTFIKFVNILNISADVLIKDITSTGKTYLENDINEELKDLNSRELNLISQIIDIIKK
                     NKI"
     gene            complement(10430..10768)
                     /locus_tag="PC414_003227"
     CDS             complement(10430..10768)
                     /locus_tag="PC414_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2465600.1"
                     /translation="MANIGKIIGDRIKVYRTKLGYSQEFLAEKAGLHPTYIGQIERAE
                     TNTSINIIMKIATALDIPLELLFANIITTEGVDNSVPLECYEMINKLTEKEQIAMLEL
                     IKCIIKYKEI"
BASE COUNT         3651 a         1743 c         1567 g         4058 t
ORIGIN      
        1 ctaatttatc aatatttcct gattgaatat gcttaatgtt tgattgaatt tttttatatg
       61 gccaatccca ccatttaatt tctaataatt ttgaaattgt atcattatta aatctctttt
      121 ttataatttt tgcaggtact cctccaacaa tagaatacgg tggaacgtca ttagtaacta
      181 ctgctcttgt tccaataatt gcaccatcac caatatttac acctgacata attatagaat
      241 cataacctat ccacacatca tttccaatta taatgttacc cttattgtcc caagcgtccg
      301 ttatatggtt gatatcgagt ttccattctt cataaaagat gggaaaagta tatgtggatt
      361 ttgatttcat tgagtgattt ccactagtca ttagaaattt tgcattacat gctattgagc
      421 aaaacttacc aattattaat ttatcattgt ttatagggta atgatacaat acattgtttt
      481 tctcaaaatc tcttgggtca ttataaaagt cattatatat tgtgtaatct ccaacttcta
      541 tattttccct tgttattaca tttttaagat atacagtttg ataatcatta cttcttggat
      601 ataccttatt tgaacttgga atagtcatat ttaaatctct cctttaatat atattatgtt
      661 tttgactatt ccactactac aatacatctc ttcacagaat cactccttgt taagaatctt
      721 cataagataa tggcgtcctt ttttagtgtt aagtcggata ttctttctta gtccattctt
      781 tttagcgtat aactgacaaa ctgtcatttt cttaccaata gtgtcaatgc tatcatcaag
      841 gtcattttgt attgcttttt ccttatccct taatgatata tcatcacgtt ttccctttgg
      901 gggtttatca gtaggtacaa gtttccaaga gtaaacaaat tgtggtttac caaatgaata
      961 tacatactta tatgcgtatc ttccatcttt tctctggctc tctccagttc ttaaaattct
     1021 atctttatta tctcgtcttt tttcagttga catgataaga agctcctctc aataaccgaa
     1081 ttgagcttga tacgtcatat gaatattata acatataagg ctcaatttca ttagataacg
     1141 tcaagtgtat caacgatttt ctcaaattgt tttctcttta tttgaatgcg attgctattc
     1201 ataatgaccc agccagaatc aaggttttca tcagcacgtt tacgtaattt tttctctcca
     1261 atacgaaagt attttgacgc ttcttcaatc gtcaaggtat atttttccca aataggtaca
     1321 tcagtattgt tcatataatg tatcactcct ttctatgttc gtagttcaga agtagataga
     1381 tggctttgat aagctccatt ggtatttcaa ccaatcccat tcttatggac agaaccgtgt
     1441 catacaggtg tatcattata ctgtggtaca ggttacggca aaacaaccgt tccaaaggtt
     1501 attcacaaat cactatatag tagctctctt gattaaggtc atggcgtata gtatttgttg
     1561 gctatctgta tcacaaacgt atctatctac tttattcagt tgtcaaagaa cagtcggcat
     1621 tgatttgcct ataaaaaatc acaatagctt gtgggctttt atagacaaat aattagtgat
     1681 tctccatgta ccaaatagct tgatacatgg agagagggcg agattaagac aaattaaatc
     1741 tcaaaatagc agtgattagt tttgttccta gcattcgctt tgtttcttct tctacatgaa
     1801 tatagggatt tccgtattca tcaaataaag ttttctgtga aagacgaatg atatagtgtt
     1861 caaaatgttt aagtattacg tttattgcat ttacatcgcc atgtacagca agtaaaataa
     1921 ttgtaaaagg taatacttct ttagtaggtt tcatttcaat gttcctgcat gagttctttc
     1981 aactctttta gtgcatttgt tttgtgataa tgtatggtat tttctgctaa atccatcaat
     2041 gttgctatgt cagcatttgt catgttgaaa aagaatgaaa gtagaattat atcttgtttt
     2101 ctttttgtaa gttttccgat tgcttgcgcc attaatacat cttcaattcg tatgttgtag
     2161 tcgtaagaaa ccatacagat gtagttagaa tcataatcat ctaaaatact caatttactt
     2221 aattctgcaa gtgacaaaga ttcgagagat accaaataat catttctgcg tttattttcg
     2281 tcataaatat ctctagcata gttacgcaaa actgttttac agaaactgtc gaattgatgt
     2341 tcaattgtct tttcatcaac cataagattt caccctcttt ccatcgcaag gagttaaata
     2401 accttgctta ctcctatcac ggtgggaggg cgttaaatct tcgataaatt tcaaaaaaaa
     2461 gagaaaaaaa tacacactct gcctataggg caaaaatgtg tattctttaa aacgtattta
     2521 atttatattg gttgtaagcc accaccatat tgatgtatgg aaatatgcat tggctagctt
     2581 ttcataaaaa tacaaagatg cataagaaat taatagaggg gaaaaactta ttctgttatt
     2641 aaaaagtagg ttaattctaa taaatgctat tgcggtcgaa ttaaactata tacatggcaa
     2701 tcaatcatgt ttctattctt tattatgaat tgtgacagta ttcttcaatc ttaaagccac
     2761 atttttccaa tactttactt gactctctat tgtaggaata tacattagca tgaattttgt
     2821 taaggttgta tttttcaaag gcaattttgc atatttcttt tactgcattt gttgctatcc
     2881 ctttgcctca atagttccta gcaagccagt aacctaattc agcattttta ttaatagagg
     2941 tatcttcttc aaatgttagg ctgatacttc caatagccct ttcttgataa aaaatagcat
     3001 agctaagttg tttaacatct gaagttgcca aacaaaaact aacaaaatct ctagcgtttt
     3061 gctccctata agggtggggg aagttgtcac gaagattctt tgcaatttct ttatcattcg
     3121 caaactcaac taaatcggta atataagcat aattccactc tataagttcc atttttcttc
     3181 tccttttatg aaacagctac aagaaatctt tctaagccaa taccacttat caaacagcgt
     3241 tctcttttat ttccaagcat ttgataagtc cagtcaacaa atccaccgtc aactatttca
     3301 acggtttcat ttccttgttt gaccgataat ttgaaattga tacctttata ataattatta
     3361 tcaacatcat cattatgaat tgataactct atattaggaa aaataagttt gatagtttca
     3421 accattctgt cgaaaaatcc gtcattatct ttgtatccgt ttcttttgcg tagaataata
     3481 gagaaacgaa cattttctat ttcacttaat atatctttat aatagtttaa gtgcttttct
     3541 aacaacattt tttcacaagt gtaagaccct gtatctatac cagatgaaac catacaatat
     3601 aagccaaaat gtgaatataa acctttacct acaaaatttt gtgctcttat taccctagca
     3661 gtagtagcca tgtggatagg tatggtatta ttttcttctt ttctttttaa tttatcagca
     3721 ataattattg ccaacatatt tgaggggtct gcaagtgttt ctgtccctcg tagagcactt
     3781 actacgttat attgatctac ggagccaaaa acgctacagc tacctaatgg ggcagaggga
     3841 gaaagcataa tcgtcttgat atccatattg aatgcttttc tcaataattt tgattccaaa
     3901 gcatgaattt cttctggatc tataccgcta gggatagtaa atctatttag ttggtatgac
     3961 tttagaatat cagttgcagt taaagtattt gcttgtctat caaacacttc taatagaagt
     4021 gagttcaaat ctgattttga tagagctgat aacttatcaa ctatattctt atccccaatt
     4081 ttatttgtaa ttctacttaa aatatcactc atagttatcc tccatactgt tactttcaaa
     4141 ataattatag aattgttaca acaactaatc taggtcattt gaccaaaaat gaaaaaacac
     4201 tgaatattta ctattcaata tttttttact ttgggtttat ttgattaaat cttgaaaata
     4261 ctgtaatgca accctttcag cttcaacctt tcggacaata ggactttgtg ataattcctc
     4321 tcttaactca agatacaggt tgatagtagc ctgcaatgca ttaacacagc ttttatattc
     4381 atggttagca accgtctttt ctaatgcttc aatatattgg ggtgcgtcca attcaatttt
     4441 tctaactcca cgaggtaatt tacctaagag aagctgtatt aatggggcaa gtactgattg
     4501 tcttaaaaaa gataaaaaat caattgtttc aaatagttct ctacgtccaa tcttagtgca
     4561 agcgtaatga acccaaatcc aaaatctatc ttcaatccac tgtaagtctg gagttgggaa
     4621 gtatgcttct tttttagaat aaatttctgt tatgcagttg ttttcttcat agagaatagc
     4681 tgaatcttca accctatcta gtaaaccatc taaggttgta aacttaaaat caacatgaag
     4741 aacaggggaa tcatagaggg aaattatcaa tcgaggttct ccaacgtgtt cgccagtaaa
     4801 agcagaaact aatgttccaa actgttccac aataagtaat ctctcttcga taacttcttc
     4861 ataggattta tcatctgcta caatcagaaa atccaaatca gaatattcat ctaacgattt
     4921 tgtgatataa gaactgctaa ttgcaagtcc tattatccta ctatctcttt ttgaaaattc
     4981 taatacgttt tgtatcattt tctgttgtgt tttagtcata atataacacc tctcactgtc
     5041 ttttattagc tatcaatcca acataattat gcataggagt attatataat gaagaaataa
     5101 aaaaaaatag gtcacttgac ccgaaataaa aaagcattga ataattatta ttcaatactt
     5161 ttttgaaatt atacttatgt tgtaggaaga ttctttaata cctctcgctg attaattaca
     5221 ctaaatcttt tgttttcata agcaattaga tgttcttgta cacattcatt tatagcacga
     5281 ttaatgcttc taattggagc gtttacttcg ctagcaagct gttttttggt aagcagattt
     5341 aattcacttc tataatagtg taaggatata cacctcagaa gtcgttcttt aacacttaaa
     5401 gatgataatt cttcaattaa ttcagcattt acaactaatt tactagatat ttctcgaatc
     5461 agaaattttg tagcttcaaa atctcccctt aaccagttta aaaaatcatc tctatgaagt
     5521 ttatatacat tacaatctgt gaaagcgaca atttcaacgg gttttttccc atcattaaat
     5581 tgttccattt ctccaaaaaa actaccactt ctataaagat gaatcgttga gatgtttcct
     5641 ttcacattta agatgaatgc ttctgcaata ccgtcaatca taagaattac atattcattt
     5701 tcttgctcag caaaaagaat agtctctttc tttttatacg tttttttaac aagcttcttt
     5761 ttaatatctt cattcataaa atcaattaaa tgcatattca tccctcctta ccaaatcttt
     5821 gattaaatta tatcatcttt caattagtta ggtaagatag taaaaatata tttacataga
     5881 aactattcca ttattatttt ccagttgtca tttttctgca aaaacttatc actgtcacga
     5941 ctggagatac cagtaaggaa tgttaggata tcaatcgcta aactttcaga atccttttta
     6001 cggctcaaaa tgacgtaagg gtcaagcaat cctttgtttt catcattact ggttaaattg
     6061 acaatgttta tctcatgggc tatttctggt agcgtttctt tccatttgcc gcgttctgct
     6121 ttagggtcaa ctatgaccgc ctgtccgccg aatagtaccg catagtaaac aagcatattg
     6181 ttactgaata atttaccacc acccaatgaa ccgataaacg cagacgctaa cgcattggta
     6241 atagaaccct ttaccccttg actagcaagg gcaggtttta gatagacatt ctttccagtg
     6301 tccagattgt agccaagata gatatcctca tgttcgccaa gggtttgtgt cgctccaaaa
     6361 ccaagtccag ccaaaaaatc cgaagtgaca tattgaatat aatcattgat gtaacgtttg
     6421 ctgacaggaa taaattcgcc atgcaatccc aacatatccc caaagggacg cacgagcttg
     6481 attgataagt cgtcgtaaaa gtctttgact tcatcacaac gcctttttag ctcgtcaaga
     6541 ttctttgtag atacacggac aacatagcta agtttataca tagattcttt acagttaaaa
     6601 gtctgttttt aaaaaaatgt ataaattata aatcaaaata tagatactat caatgaccca
     6661 ctaggtgcaa aaagcattga tatggttaaa ttatcagacg ctaaaagctg ggctatccga
     6721 atgagtgaag atgggtgtgc ttataaatcc attgacaatt ataagcgctc actgaaagcg
     6781 tcgtttttca tggcaattca agacgactgt attagaaaga atccatttaa tttttagtta
     6841 agtgatgttt tggaagatac aacagaacca aaaatcgctc taacaccaga acaggaaatg
     6901 ggcttattat cctttatggg gagcgataag acttattgta aatattatga tgatgttgtt
     6961 atcctcttag aaacaggact acgcatttct gaattatgcg gtttgacttt agaattggat
     7021 atgaaaaata aagccattat ggtagaccat caattgttga gtgatactaa gattggctat
     7081 tatgtagaag ttccaaagac cacacagggt aagcgagaaa ttccaatgac tgaaagagct
     7141 tatcaatcat ttgaacgtat cgtggaacgg cgtaagaacg ctaaagcaga accaatcatt
     7201 gttgatggtt accagaactt cctatttctt aaacaagatg gattaccaaa acaagcaact
     7261 cattatcaaa gcatgttaag agcgttagtc aagaagtata ataagaccca tgaggacaag
     7321 ctaccaaaga ttacgccaca tacctttagg catacactct gtacaaatat ggctaacaaa
     7381 ggaatgagtc ctaatatatt gcaatatatt atggggcata aaaacattac tatgacgctt
     7441 ggctattatg cacatggttc atttagttct tcgagagctg aaatggaacg gttataatcc
     7501 tagtagtatg actagagttt actacttatt tactactttt gatggcattc ttatacggtt
     7561 aaatgccaac ttatgcaaag tatcttccaa aaagaaaatg ccgatactcc ttgtagatac
     7621 aggatatatc gacatttacc aacttatgaa aagataatca aatttaatat aagtttttca
     7681 aataaaaaga taccaaattc tttttcgaga atctatataa cattacttaa gcttggttgg
     7741 ggtataaaca attgagtagc tacttttgta tggtgttcaa ctttagtcaa aactcttaag
     7801 taatgcaaat gatttagatt cattaatatt caatctcttc ataagtagtg aaattcgagt
     7861 tatttctgca taattatact atgatttata gattaattct attagcatcc agaataatat
     7921 tgtttatatg agtaaaattt aaagaatatt gtgaaaatta taggatgtta aaataaattt
     7981 aggtaaaaaa gaagtgtttg tatattatag agtacctttt ctaaaatatg tgggattgtt
     8041 ttaagaatta gaataaaaat tatttttaaa ttagtatttt tataatgtat agtattttta
     8101 atgtataatt aaattgtgca gaaaatagaa tttaaagtcc tatagaattt ctgattatgg
     8161 aggagaatat gaaaagaaaa gttcaagtaa aaaatattac tattggagaa ggtagaccta
     8221 aaatttgtgt accaattatt gggaaaaata aaaaagatat tataaaagag gctaaagagt
     8281 taaaagatgc atgtttggat attatagagt ggcgagttga tttttttgag aatgtagaaa
     8341 atattaaaga agttaaggaa gttctctatg aattaagaag ttatatacat gatatccctt
     8401 tattattcac atttagaagt gtagtagaag ggggagaaaa attaatatca agagattatt
     8461 atactacttt gaataaagaa atttctaata caggtttagt agatttaata gatgtagagt
     8521 tatttatggg agatgaggtc atagatgaag tagttaattt tgctcataaa aaagaagtga
     8581 aagtaattat atctaatcat gattttaata aaactccaaa aaaagaagag attgtatctc
     8641 gtttatgtag aatgcaggaa ttgggcgcag atttacccaa aatagctgtt atgccgcaaa
     8701 atgaaaaaga tgtgttagtg ctacttgaag ctacaaatga aatgtttaaa atttatgcag
     8761 atagaccaat aataacaatg tcaatgtcag gaatgggtgt tataagtaga ttgtgtggtg
     8821 aaatatttgg ttctgcattg acttttggag ctgcaaaaag tgtttcagca ccagggcaaa
     8881 tatcatttaa agagttaaat tcagtactta atctattaca taagagtatt aattaaatta
     8941 attatataga aatattagac ggtatttttg aagatataaa ttaaatataa aaaacttttc
     9001 agatagtaaa atttaaattg ggcaattata tctgaaaagc aactatttag tgtgtatttt
     9061 gaaagtgata tagaaaatct tataaatcaa ctatggcata tttaaaatat gccattaaaa
     9121 cctactagac attagattat agatggtata ctagcatttc tattatttat agatagtaag
     9181 ttttcataat aactgtttta tctaaagtag aagatatatt tgtattatgt atatttgtca
     9241 ttattttttt gtaattatgt atattaatgg ttatatatgt aatataatat tatcactcta
     9301 ttttttgtat attgtacatt acaacattta tatctatcat catcaattac agtagaatgt
     9361 atgggatact tattttcact taaccaatat ttttgcatat tctttgtcca agactttgga
     9421 aacttcaata ctttcttgtt tattataaca tcctcatctt caaaaatatt aacttctctt
     9481 tctattttta tttttctgtt gtatatctta ttgatagatt ctttgtcgag ttttatgtct
     9541 ctatacatat cagggcaata gcaacaaaca taatttcttt tttttcgacg tttaaatttc
     9601 acaactttct ctatgctaat ttctaatttt tttgtaccat cttttataaa tttaatatct
     9661 tctatgttct cgcttgaaaa aatttctgtc aatcttaaag tatctacagt ttctctaatt
     9721 atttgctttt tataagaaga gaatccatat ccaaaagaaa ggtttaattt attagggatg
     9781 tagccatttt tatatttaaa catcttaatt ttttccaatt cagcattatc tttaaatttc
     9841 aacttttcat acctaaaagt tactttgcta aaaagagtat attctacatc aatatattct
     9901 tttgcaatct taaatttatc gctaccttca atcacaatca tagcctatct ccaatcgttt
     9961 tatattaaaa taaggccata gaacatggtc taatagtact cattagcact ataagacaat
    10021 gttaacacaa aaagacaaaa aaaggttatt tatttcaaat aacctttttt tttatataaa
    10081 atataaagaa ttatatttta ttctttttta tgatgtctat aatctgtgat atcagattta
    10141 gttctcttga atttaagtct tttaactctt catttatatc attttctaaa tatgtttttc
    10201 cagtggaagt aatatctttt ataagaacat ctgcagaaat atttaatata tttacaaatt
    10261 ttatgaatgt tttaagactt ggattctttt taccactttc gatttgagtt atataccaag
    10321 gcgatactcc aaccatattt gcaaattctt ttgaagttaa atttgctttt ttccgttctt
    10381 ctttaagtct tttacctata taattcatta ttgttactat ttccctcact tatatttctt
    10441 tgtatttaat gatgcacttt attaactcta gcattgcaat ttgttctttt tcagttaatt
    10501 tattaatcat ttcataacat tctaaaggta cagagttatc aacgccttct gttgtaataa
    10561 tatttgcaaa taacaattca agtggtatgt ctaaagcagt agcaattttc attataatat
    10621 tgatagatgt atttgtctca gctctttcaa tctgaccaat atatgttgga tgtaaacctg
    10681 ccttttctgc tagaaactct tgactatatc ccaattttgt tctatagact tttattctat
    10741 ctcctattat ttttcctata tttgccaatt attatccctc cactttagat tatattcata
    10801 gtttaagttg ttttatatag actatttata cttttttagt ctaaatatag actatatatg
    10861 tttttatact gaaataagtg ctatttattt ttgtatagtc tgtatctaca ataaacaaat
    10921 atggataaat catattttta attagagaaa tattttcata atttcatagc catactacaa
    10981 taataaaacg aattaataat atgttaatta tagcatttt
//
LOCUS       DAMJVK010000114        11009 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.119, whole genome shotgun sequence.
ACCESSION   DAMJVK010000114 DAMJVK010000000
VERSION     DAMJVK010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11009
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.119"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(207..1937)
                     /gene="lpdA"
                     /locus_tag="PC414_003228"
     CDS             complement(207..1937)
                     /gene="lpdA"
                     /locus_tag="PC414_003228"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDF2465601.1"
                     /translation="MSVEVIMPKAGVAMEEGTIVSWLKQEGEEVKIGEPILEITTDKV
                     NMEIESEGEGTLAVIIHKEEGEVLPVFTVIGVIAEKGENQEEVKAKYLSGNVSKEDIV
                     EENQNIEFKEEKINKKECNHDYDVVVIGGGPGGYLSALKAALLGGRVALVEENILGGT
                     CLNRGCIPTKTYIKTAEILEEIDQLSKRGVKVTVDKEQDIKKAIKYKNRVVKKLTAGV
                     GGLLKSRDVDVFNLKASVKEEHKVILSDGKVLDTENIIIATGSKVRILPIKGIESNLI
                     ITSTEALDLETVPEELVIIGGGVIGCEFAEIFNSRGSKVTIVEMEDRVIPRMDKELSE
                     SLKYSLSKKGINVLTKKKVSEFKEEGNNILVCIEGEEPIKADLCLYAIGREANLSGIE
                     DLDIKIDKGSIVVNSKMETSIPSIYAVGDVTGGVMLAHAAFKMGEVAASNALGVNKEV
                     DLGALPSCVYTIPEVASVGITEEDARKKYNVKVGKFNFAGNGRALASGQEQGYVKVVA
                     DAKYGEILGIHMFGCGVAELINHAASFKALEIPTDEASELIFGHPCTSEALMEALADV
                     NGECLHLPKK"
     gene            complement(2104..3150)
                     /locus_tag="PC414_003229"
     CDS             complement(2104..3150)
                     /locus_tag="PC414_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HDF2465602.1"
                     /translation="MVANKIKATPAARSQARKDNIKLDRLIGSGENGRIHLVDVLNYL
                     KDNKANTTPLARRIAEDLNIDLETIVGTGYNGKIRKCDVEKLTAKETIVSTNTSKSSE
                     KKELKIENENSSMFNTVEGIFEKPNPMRATVAKRMSESYFSAPVFTFNIEVDATELKV
                     LRAKLIDTVKESTGVKLTMTDLIVMAVSKILPNHQALNSAWTDEGIFRYKDVNIAIAV
                     GLDEGLYVPVVKNANKKSLKEIAKESKELAEKVKTGKLMPADQEGNTFTISNVGMYGI
                     TTFTPIINMPSSAILGVGATQDKFVPVNGEAKIKPIMNLSLTSDHRVIDGTVAAKFLK
                     DLKELLENPLSMLV"
     gene            complement(3163..4149)
                     /locus_tag="PC414_003230"
     CDS             complement(3163..4149)
                     /locus_tag="PC414_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HDF2465603.1"
                     /translation="MSTRELTYAQAIKEAMSEEMRRDENVIFMGEDIGIYGGAFGVSV
                     GMIDEFGPERVRDTPISEAAIAGAAAGAAATGLRPIMEVMFMDFVTISMDAIVNQAAK
                     MRYMFGGKAQVPMVVRCPGGSGTGSAEQHSQSLEAWFCHVPGVKVVAPSTPADAKGLL
                     KAAIRDNNPVIFVENKLLYRKKGFVPEDDYVIEIGKADIKREGTDVTVITYGRMLQSV
                     EEAAETLSKENINVEIIDLRTLYPLDKETIVKSVCKTGRVLICHEAAKTGGLGGEISA
                     LITESESFDYLDAPVKRICGKDVPIPYNPELEKAVVPRVDEIEEAIKSLIVR"
     gene            complement(4174..5136)
                     /locus_tag="PC414_003231"
     CDS             complement(4174..5136)
                     /locus_tag="PC414_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HDF2465604.1"
                     /translation="MSISKETLLEMYKRMNQARKFEEKVSWFFARGMVHGTTHLSVGQ
                     EASSVAAVMALEKGDLVSLTHRGHSQFIGMGIDLNKMMAELMGKETGFCKGKGGSMHI
                     ADIESGNLGANGVVGGGLTIAPGAALTQQYKKTGKIVLCSFGDGASNEGTFHEGINLS
                     SIWKLPIIFYCENNLYGMSTSIKRHMNIESIATRAASYGIEGISIDGYNPIEVYETVQ
                     KAAEKCRRGEGPVLIESRTYRWLGHSKSDANVYRTKEEIESWKAKDPIEFLKNYLIEN
                     NLSNEDELDKIQEFAKQSIEDAVEFAQNSPNPKIESLLEDVYAD"
     gene            complement(5174..6127)
                     /locus_tag="PC414_003232"
     CDS             complement(5174..6127)
                     /locus_tag="PC414_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HDF2465605.1"
                     /translation="MKKIGDLRLMMKCCSLYYEDNLNQQEIANQLGISRPTISRILKE
                     AFEQGIVKIQIVDVLKNDYQKIERSLERKYKLKEVIVVDDKQDALTQKQELARAVSEY
                     LTRVVKENDIIGVSIGTTLKEIPRYVEKSNCKNVTFIPLLGGIGDNEIDIHANQIAVS
                     LARAFGGDFKLLHAPAVMSDLSTKEKLCKDEKIKEVLDLIDKTTIAIVGIGNPMSLNS
                     TIMASGYMNEADIEDLKKYNSIGAICLQAFDKEGKTSILEFNQRVLGVKLEDLKKIKR
                     TIGVASGDEKIEAIKASLKAKFINSLAINHSLALKLLEDCD"
     gene            complement(6572..7945)
                     /locus_tag="PC414_003233"
     CDS             complement(6572..7945)
                     /locus_tag="PC414_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2465606.1"
                     /translation="MNTEVNVKATTIRYEKISFFERISYGCGDLGCNIIYSAMSAFLL
                     FYYTNYADVSAAAVGSIMLVSRILDGFSDLTMGIIVDRTKSKYGKARPWILRMAIPFA
                     IAAVLLFSVPSNLGITSKLIYIFITYNLVSTVIYTAINVPYATLNSLITQNQYERGVL
                     SIFRMILATCGTLLINGLTLPLVEYFGNNLSAWTKTFFVFGIASIMVFFITFTGTKER
                     VKAVKQDRNEVIPFKIGIKSLFRNKYWIQITLCLVCIFIVFAINGGSSVYYAKFILGD
                     EKLFGPINMVSNISQIIAMFMVAPFIKKFGKRNVLIVGSIILISSNVMFIIVGQNHIG
                     VIFASAIKGIGSAGIASTMFAIVSDTIEYGEWKTGYRTEGLINSASSFGFKVGNGLGS
                     AILGAVLSIGGYVGTSATQSDLAIVSIKVCFIYLPIFITILQIIIMSFYKLDKEYSTI
                     LNELNTK"
     gene            complement(8042..9688)
                     /locus_tag="PC414_003234"
     CDS             complement(8042..9688)
                     /locus_tag="PC414_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HDF2465607.1"
                     /translation="MIKNPILPGFNPDPCICRKGDDYYLVVSSFEWFPGIPVYHSKDL
                     KNWELYTHILTDETKIDLKKLPSSKGIWAPCLTYCEEEDLFYIVYGIMNSMNARYFDV
                     DNYLITSKDIKGEWSEPVYLHSSGFDASIFHDDDGKKWIASLDWETREGYEKPGVICL
                     VEYCTKKKEIVGYPKRIWSGGTDRGCIEAPHITKRGDYYYIMCAEGGTGYGHSVTMGR
                     AKNIWGPYEKDSMNPIVTSIPGDFYERHDPDHLKPKYYNPESKLQKSGHGSYIETTSG
                     EVYLVHLTSRPFVPELRCTLGRETAIQKMKWTKDNWLRMEDESNLAKEYVSESKLEEH
                     LVSSIPSFDDFDSNELGLQYYAPRISPLSFADVKSRPGYVRIRGQESRTSLNKVSILA
                     RKLTSVYARITTKMEFYPEVHQHSAGLIMYYDNMNYINLRKYYSETLGQSALSIIHLE
                     NGEKTEFLNTRIPIKDIPIYLRLYVQGRKSYFEWSYDEKNYQRIGKVFDTTKFSDEYC
                     KYGEFTGTFIGLTCADRVKHKHYADFDFFEYIVDESKDVD"
     gene            complement(9710..10876)
                     /locus_tag="PC414_003235"
     CDS             complement(9710..10876)
                     /locus_tag="PC414_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HDF2465608.1"
                     /translation="MYIKGVNLGGWLVLEKWMTSSLFEGTEAEDEYYLPRQLSREAYE
                     SRIKTHRSEYITERDFAMIKSMGFNSVRIPVPYFIFGDCEPFIGCVKELDKAFAWADK
                     YGLSILIDLHTVPGSQNGFDNGGISGICSWSQNPEYVAFTLNVLERLAKRYGMRHELY
                     GIQILNEPITERMWNIMNVPNRFKAVDKEMARGSKPNSLEFLRDFYIKAYKVMRPYMR
                     EENVIVFHDAFELKAWKDFMREEEFKNVVLDTHQYLMLAEADGCEQSIDSYLKYIREN
                     YAKDILQMQKYFPVICGEWSLFNSYACGIDTNGGQSPLNGIESNIDKLSKDDKRELYR
                     KIAKAQLDAWRNGSGHYYWNYKLLLDTVNEEGWIGWDSWDLVKCVAQEWYPIEY"
BASE COUNT         3278 a         2201 c         1175 g         4355 t
ORIGIN      
        1 aatgataatg ttattttata ttactatctt ttttgtacaa caaaaactac aaggattcta
       61 tattctatat ttttccataa aaaatacccc ttattatgca aataaattat ttgcataata
      121 aggggtatgg ctaagtaagt gttttaaaat tttatgatat aaattaatat taataataga
      181 cttaaattaa taatttatgt ttagatttat tttttaggta agtgtaaaca ttctccattt
      241 acatctgcca aagcttccat taaagcttct gaagtacatg gatgaccaaa tatcaactca
      301 cttgcttcat cagtaggtat ctctaatgct ttaaatgatg ctgcatgatt tataagttct
      361 gctacaccac atccaaacat atgtatacct aaaatttctc catatttagc atctgctact
      421 accttcacat atccttgttc ttgaccagaa gctaaagctc ttccatttcc agcaaaatta
      481 aacttaccaa cttttacatt atattttttt ctagcatcct cttctgtaat accaactgat
      541 gctacttctg gaattgtata aacacaacta ggtaatgcac ctaagtcaac ttctttattc
      601 acacctagag catttgaagc tgctacctct cccattttaa aagcagcatg agctaacatt
      661 acacctccag taacatcacc tactgcatat atgcttggta tacttgtttc cattttacta
      721 tttacaacta ttgaaccttt atctatttta atatctaagt cctctatgcc agaaagatta
      781 gcttctcttc ctatagcata tagacataaa tctgctttta ttggctcttc accttcaatg
      841 cacactaata tgttatttcc ttcttcttta aattcagaaa ctttcttttt agttaataca
      901 tttatacctt ttttactaag agaatatttt aaagattcgc ttaactcttt atccattctt
      961 gggattactc tatcttccat ttctactata gttactttag aacctcttga attgaatatc
     1021 tctgcaaatt cacaaccaat cactccacca ccaattatta ctaattcttc tggcactgtt
     1081 tccaaatcta atgcttctgt actagttatt attaaatttg attctattcc ttttataggt
     1141 aatatcctta ctttggaacc tgtagcaatt attatatttt ctgtatctaa aacttttcca
     1201 tctgataaaa ttactttatg ctcttcttta acactagctt taagattaaa cacatctaca
     1261 tctctacttt ttaataatcc gccaacacca gctgtaagtt ttttaactac tctattttta
     1321 tattttatgg ccttcttaat gtcctgttct ttatctacag ttacttttac acctctcttt
     1381 gataattggt caatttcttc taatatttct gctgttttta tataagtctt tgtagggata
     1441 caacctctat ttaagcaagt acctcctagt atattttctt caactaaagc aactcttcca
     1501 cctaaaagag ctgcttttaa tgctgataaa tatcccccag gtcctcctcc tattaccact
     1561 acatcataat catgattaca ctcttttttg tttatttttt cttctttaaa ttctatattt
     1621 tgattctctt caactatatc ttcttttgaa acatttccag ataaatattt agcttttact
     1681 tcctcttgat tttccccttt ttctgctatt acacctatta ctgtaaatac tggtaatact
     1741 tcaccttcct ctttatgtat gattacagca agtgtacctt ctccttctga ctctatctcc
     1801 atattaactt tatctgttgt tatttctagt ataggttctc caatctttac ttcttcgcct
     1861 tcttgcttta gccaagatac tatagtacct tcttccatgg ctactcctgc ttttggcatt
     1921 attacttcta ctgacatagg ctctcctcct atttattttc ttatatttta attaagcaaa
     1981 tcatattcac atatagttac tgttaaaaat aattcttaat atataaaagc tttaaattta
     2041 ctgaaacaat tataataata taatctattc aaactattta tatcagtaac aaaatatcaa
     2101 aacctataca agcattgata aaggattttc taacaattct tttaaatctt ttaagaattt
     2161 tgcagctact gttccatcta ttactctatg gtctgatgtt aatgataagt tcattatagg
     2221 ttttatttta gcctctccat ttactggaac aaacttatct tgtgtagcac ctacccctaa
     2281 gattgcactt gaaggcatat ttattatagg tgtaaatgta gttataccat acattccaac
     2341 attacttatt gtaaatgtat taccttcttg gtctgctggc ataagtttcc ctgtctttac
     2401 tttttccgca agttctttgc tttcttttgc tatttctttt aaagactttt tgtttgcatt
     2461 ttttactacc ggaacatata agccttcatc taaaccaact gcaatagcta tatttacatc
     2521 tttatatcta aatataccct catctgtcca tgctgaattt aaggcttgat gatttggaag
     2581 tattttagat actgccatta ctattaaatc tgtcatagtt aattttactc cagttgattc
     2641 ttttactgtg tcgattaact ttgctctaag taccttcaat tctgtagcat ctacttctat
     2701 attgaatgta aatacaggag cactaaaata actttctgac attcgttttg ctacagtagc
     2761 tctcattgga tttggctttt caaatattcc ttctactgtg ttaaacatgc ttgaattttc
     2821 attttcaatt ttcagctctt ttttctcact agatttagat gtattagtcg atacaatagt
     2881 ttcttttgca gttaattttt ccacatcaca ctttcttatc tttccattat atccagtacc
     2941 tacaatagtt tctaaatcta tatttaaatc ttctgctatt cttctagcta aaggagtagt
     3001 atttgcttta ttatctttta aataatttaa cacatctact aaatgaatcc ttccattttc
     3061 tccacttcca atcagcctat ctaattttat attgtctttt cttgcctgac ttctagctgc
     3121 tggtgtggct tttattttat ttgccaccat atctatccct ccttatctaa caattaaaga
     3181 ttttattgct tcttctattt catcaactct tgggacaact gctttttcta actctggatt
     3241 atatggtatt ggcacatctt ttccacaaat tctttttact ggagcatcta aataatcaaa
     3301 tgattcactt tctgttatca gtgctgatat ttctccacct agcccaccag tcttagctgc
     3361 ttcatggcaa attaaaactc tcccagtttt acatacactc tttactatag tctctttatc
     3421 taatggatat aaagttctta aatctataat ttcaacattt atattttctt ttgatagagt
     3481 ctctgctgct tcttcaacac tttgtaacat tcttccataa gtaattactg taacatcagt
     3541 accctctctt ttaatatctg cctttccaat ttcaattaca tagtcatctt ctggcacaaa
     3601 accttttttt ctgtataaca atttattctc aacaaatatg actggattat tatctcttat
     3661 agctgctttc aacaatccct tagcatctgc tggtgtagaa ggtgcaacta cttttactcc
     3721 tggtacatga caaaaccaag cttctaaact ttgtgaatgt tgctctgctg aaccagttcc
     3781 agaacctcct gggcatctaa caaccattgg aacttgtgct tttcctccaa acatatatct
     3841 catttttgcc gcttgattta caattgcatc catagaaatt gttacaaagt ccataaacat
     3901 tacttccata ataggtctta agccagttgc tgctgcacct gctgccgctc ctgctatagc
     3961 tgcttctgat ataggagtat cccttactct ttctggtcca aattcatcta tcataccaac
     4021 tgatacacca aatgctcctc catatattcc tatatcttct cccataaata ttacattttc
     4081 atctcttctc atttcttctg acattgcttc ctttattgct tgtgcatatg ttaattccct
     4141 tgtactcatt tttattttcc tcctttacca ttcttagtct gcatatacat cctctaataa
     4201 agactctatt tttggatttg ggctattttg agcaaactct actgcatctt ctattgattg
     4261 ctttgcaaat tcttgaatct tatctaattc atcttcatta gatagattat tttctataag
     4321 ataatttttt aaaaattcaa tagggtcttt tgctttccat gattctattt cttcttttgt
     4381 tctatataca tttgcatctg attttgaatg acctaaccat ctataagttc tactttcaat
     4441 taatacaggt ccttctcctc ttctacactt ctcagcagct ttttgtactg tttcgtatac
     4501 ttctattggg ttatatccat ctattgatat tccttctatt ccataagaag ctgctctagt
     4561 ggcaatactt tctatattca tatgacgttt tatgcttgtt gacataccgt ataaattatt
     4621 ctcacagtaa aatattattg gcaatttcca tattgaagat aaatttattc cttcatgaaa
     4681 agtaccttca tttgatgctc catcaccaaa actacataaa actattttgc ctgttttttt
     4741 atactgttgt gtaagtgcag ctcctggagc tattgtaaga cctccaccta caactccatt
     4801 tgctcctaag ttacctgact caatatctgc tatatgcatt gagcctcctt ttcccttaca
     4861 aaatccagtt tctttaccca taagttcagc catcatttta tttaggtcta ttcccattcc
     4921 tataaattga ctatgtcctc tatgagttaa tgatactaaa tctccttttt ctaaagccat
     4981 tacagccgcc acacttgacg cttcttgacc tacagataag tgagttgttc catgaaccat
     5041 acctcttgca aaaaaccaac taactttctc ttcaaatttt ctagcctgat tcattctctt
     5101 atacatttca agcaatgttt cttttgatat tgacatatac attccctcct gttaactttt
     5161 atctatcttt ctattagtca caatcttcta ataatttaag tgctaaactg tgattaattg
     5221 ctaaactatt tataaatttc gcttttaatg atgcctttat tgcctctatt ttttcatctc
     5281 ctgaagcgac acctattgtt ctttttatct tttttaaatc ttccagtttc actcctaata
     5341 ctctttgatt aaattcaagt atagaagttt ttccttcttt atcaaaggct tgtaaacaaa
     5401 ttgcacctat tgagttgtat ttttttaaat cttctatatc tgcttcattc atgtatccag
     5461 aagccattat tgttgagttt aaactcatag gatttcctat acccacaatt gctatagttg
     5521 ttttatctat taaatctagt acttctttaa ttttttcgtc tttgcaaagt ttctcttttg
     5581 tagacaagtc agacataaca gctggagcat gtaataattt aaaatcacct ccaaaagccc
     5641 tcgctaaact tacggctatt tgatttgcat gaatatctat ttcattgtct ccaatccctc
     5701 ctaatagagg tataaatgta acatttttac agttactttt ttccacatat cttggtattt
     5761 cttttagtgt tgttcctatt gaaacaccta ttatatcatt ttcctttact actctagtta
     5821 aatattcaga tacagctctt gctagttctt gtttttgtgt tagtgcatcc tgtttatcat
     5881 ctactactat tacttctttt aatttatatt ttctctccaa acttctctct attttttggt
     5941 aatcattttt caaaacatca actatctgta tttttactat tccttgttca aaagcttctt
     6001 ttaatattct tgatattgtt ggtcttgata ttcccaattg atttgctatt tcctgttgat
     6061 tcaaattatc ttcgtaataa agtgaacaac acttcatcat taatcttaag tctccaattt
     6121 ttttcatttg ctttcctcca aaatcaagct tttcaattat ctttacattt gttcaatcat
     6181 tttacttaat ttcagtaaca ttatagaagg ggttcatata aaaagtcaac tgtttttttg
     6241 tataacaaaa aaaaggtgat acttttcttg taccaccacc ttcataaata gttgaaaaat
     6301 tgcttcttag tgcatattat atattacttc taattgttaa atatcatctc cataaattaa
     6361 aaattaattg tccacttata atgctttaaa taacaataac ttattaaatc acttctaaaa
     6421 aaccaaccat ttcatcattt aaagtcttct tatattttta ttattcgctt ctctaaaaat
     6481 tcttcaataa tcataaatat ataatagttt ttatagggaa catttacaat agaccttaat
     6541 taaacttaag actttataag aaactttttc attatttagt atttaattca ttaagtatag
     6601 tactatattc tttatcaagc ttataaaaag acattatgat aatttgtaat attgttataa
     6661 atataggtag gtatataaaa catactttta ttgatactat agctaaatca ctttgagtag
     6721 ctgatgttcc tacataacca ccaattgata atactgcacc taatattgct gaacctaatc
     6781 catttcccac tttaaaccca aaactagatg cactatttat aagcccctca gttctatatc
     6841 cagtcttcca ttctccatat tcaatagtat ctgaaacaat agcaaacatt gttgatgcta
     6901 ttccagcact tcctattccc tttatagcac ttgcaaatat tactcctata tgattttgtc
     6961 caactattat aaacataaca tttgacgata ttaatattat tgaacctact attaatacat
     7021 ttctttttcc aaattttttt ataaaaggag caaccataaa catagctata atttgggata
     7081 tatttgaaac catatttata ggaccaaata atttttcatc tcccaaaata aactttgcat
     7141 aatatacgga tgaaccacca tttatcgcaa atacaataaa tatgcaaact aaacataatg
     7201 ttatttgtat ccagtactta tttctaaata aagatttaat acctatttta aatggtatta
     7261 cttcattcct atcttgctta actgctttta ctctttcctt ggttccagta aatgttataa
     7321 aaaataccat tatagatgct attccaaata caaagaatgt tttagtccat gctgataaat
     7381 tatttccaaa gtattcaact agaggtagtg taagtccatt tattagtaag gtaccacaag
     7441 tggctaatat cattctaaat atacttaata ctcctctttc atactggttt tgagttataa
     7501 gtgaatttag agttgcatag ggtacattga ttgctgtata aataacagta gaaactaagt
     7561 tatatgtaat aaaaatataa attaatttag aagtgatacc aagatttgat ggaacagaaa
     7621 ataataatac tgctgctata gcaaaaggaa tcgccattct caaaatccaa ggcctagcct
     7681 taccgtattt ggatttagtt ctatccacta taattcccat tgttaagtca ctaaacccat
     7741 ccagtatcct agatacaagc ataatactac ctacagctgc tgcactaaca tctgcataat
     7801 tagtataata aaacagcaaa aatgctgaca tagctgagta tatgatattg caccccaaat
     7861 ccccacaacc ataagaaatt ctttcaaaaa atgaaatctt ttcatatcta atcgtagttg
     7921 ctttaacatt aacttctgta ttcataatac catactcctt tttgtaagat ttgccttcta
     7981 atgtttctta tttcagagca taagatttac ctttgctctg aaaattttag taatgatttt
     8041 attaatcgac atcttttgat tcatctacta tatattcaaa aaaatcaaaa tcagcatagt
     8101 gtttatgctt tacacgatct gcacaagtta atcctataaa cgtacctgta aattctccat
     8161 atttacagta ctcatctgaa aacttagttg tatcaaatac ctttcctatt ctctggtagt
     8221 ttttttcatc ataactccat tcaaaatatg acttacgacc ttgtacatac agacgaagat
     8281 atattggtat atcttttatt ggtatcctag tatttaaaaa ttccgttttc tctccatttt
     8341 caagatgtat tattgatagt gcactttgac ccaatgtttc actataatac tttcttaaat
     8401 ttatataatt catattatca taatacatta tcaaacccgc actatgctga tgtacctctg
     8461 ggtaaaactc catcttagtg gttattcttg catatactga agttaatttt cttgccaata
     8521 tgctcacttt atttaatgat gttcttgatt cttgtcctct aatccttaca tatcctggtc
     8581 tagacttaac atctgcaaat gataatggtg aaatcctagg agcatagtac tgtaaaccta
     8641 attcatttga atcaaagtca tcaaaacttg gtatagatga aactaaatgt tcttctagtt
     8701 tactttctga tacgtattct tttgccaaat tactttcatc ttccattcga agccaattat
     8761 cctttgtcca tttcattttt tgaatagctg tttctctacc taaagtacac cttaactctg
     8821 gtacaaatgg tctagatgtt agatgaacta aatatacttc acctgatgtt gtttctatat
     8881 agcttccatg accagatttt tgaagttttg attctggatt atagtattta ggctttagat
     8941 ggtcagggtc atgtctttca taaaaatcgc ctggaattga agttacaatt ggattcattg
     9001 aatctttttc ataaggtccc catatatttt tagcccttcc catagttaca ctatgaccat
     9061 atccagttcc accttctgca cacattatat aatagtaatc tccacgcttt gttatatgtg
     9121 gtgcttcaat acaacctcta tcagttcccc ctgaccatat tctctttgga tatcctacta
     9181 tttctttctt ttttgtacag tattccacta aacatataac accaggtttt tcatatcctt
     9241 ctcttgtctc ccagtctaat gatgcaatcc actttttacc atcatcatca tgaaaaatag
     9301 acgcatcaaa acctgatgag tgtagatata ctggttcact ccactctccc tttatatctt
     9361 ttgatgtaat taaataatta tcaacatcaa aatatcttgc attcatagag ttcataattc
     9421 cataaactat ataaaataaa tcttcttctt cacaataagt caaacagggt gcccaaattc
     9481 ctttagatga aggaagcttc ttcaaatcta tttttgtttc atctgtcaaa atatgagtat
     9541 ataactccca attttttaag tctttagagt gataaacagg tatacctgga aaccattcaa
     9601 aagatgaaac tacaagataa taatcatctc cctttctaca aatacaagga tctggattaa
     9661 aacctggaag tatagggttt ttaatcatat cttttcctcc aattttagtt taatattcta
     9721 taggatacca ttcttgagct acacatttga ctaggtccca actatcccat cctatccaac
     9781 cttcttcatt aactgtatct agtaataact tataattcca atagtagtga ccacttccat
     9841 ttctccatgc atcaagctgt gcttttgcta tcttcctata taattctctt ttatcgtctt
     9901 ttgataattt atctatattt gattcaatcc catttagagg tgattgacct ccatttgtat
     9961 ctataccaca agcatatgaa ttaaataagc tccactcccc acatataaca ggaaaatatt
    10021 tttgcatttg taaaatatct ttagcataat tttccctaat atatttgaga tagctatcaa
    10081 tactttgttc acatccatct gcttcagcta acatcaaata ttggtgtgta tctagaacaa
    10141 catttttaaa ttcttcctct ctcataaagt ctttccatgc ttttaattca aatgcatcat
    10201 gaaatactat aacgttttcc tccctcatat aaggacgcat taccttatat gcttttatat
    10261 aaaaatcacg taaaaactct aatgaattag gtttactccc ccttgccatc tccttatcta
    10321 cagccttaaa ccgatttgga acattcatta tgttccacat tctttctgtt ataggctcat
    10381 ttaaaatttg gattccatac aattcatgcc tcatcccata acgttttgca agtctttcta
    10441 atacatttaa tgtaaacgct acatattctg gattttgtga ccagctacat atacctgata
    10501 ttcctccatt atcaaatcca ttttggcttc ctggaactgt atgtaagtct atcaatatac
    10561 taagcccata cttatctgcc caagcaaatg ctttatcaag ttctttaaca catcctatga
    10621 atggttcaca atctccaaaa ataaagtatg gtactggtat acgaactgaa ttaaatccca
    10681 tagatttaat cattgcaaaa tctctctctg tgatgtattc actacgatga gtcttaattc
    10741 tagattcata tgcctctctt gaaagctgtc ttggtaaata gtattcatct tcagcttctg
    10801 tgccttcaaa taaacttgag gtcatccatt tttctaaaac taaccaacct cctaaattta
    10861 caccttttat atacacataa aatcctcccc tcttatctta tttaaatagg tatattttgc
    10921 acttataata aaataatttt tttaagttat tttaatatac agttattata tttgatttta
    10981 tccgttttct taagtatgtt tattatcta
//
LOCUS       DAMJVK010000115        10912 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.120, whole genome shotgun sequence.
ACCESSION   DAMJVK010000115 DAMJVK010000000
VERSION     DAMJVK010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10912
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.120"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(688..1422)
                     /locus_tag="PC414_003236"
     CDS             complement(688..1422)
                     /locus_tag="PC414_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2465609.1"
                     /translation="MNLAKGLDVLKISSNVFGEDKVMYIPVIYTEDDATLIDTGLPGQ
                     GDLIIDALNKSNTSFDKLKNIIITHHDIDHIGNINYLREKSKNNIKVYAYKSEISYIT
                     GEETPFKLYMLEQMVDKIDDKMLSMLNVMGLGFKSSYTKVDVSLDNHEKLNLGEEIEV
                     IHTGGHTRGHICLYLKESKVLIAGDLLQVENGELKPVDVMHSNKQELKDAIKNISNYD
                     IETIVFSHGGLYQRNIIGTLKNLIIE"
     gene            complement(2433..3071)
                     /locus_tag="PC414_003237"
     CDS             complement(2433..3071)
                     /locus_tag="PC414_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HDF2465610.1"
                     /translation="MLIHLVKKDILIVKKYVLLILFIAIGIPLFTLWRLPPFAQILGF
                     VLSIIFSEFMLCQYLLLKESQYSKASVLLCSTPYPRRGLVQSKYVLFAMVFIYCTLVY
                     WIENLLIPQIGTFDLTYILIVLLISSIIYGVYMPIQYKLGYETTKFFFVVIIMASSFV
                     LPLTIAHSNFEIPFIYQYPSMIVNIVLFLLSLIILLASMFISIKIFSNKELV"
     gene            complement(3074..3931)
                     /locus_tag="PC414_003238"
     CDS             complement(3074..3931)
                     /locus_tag="PC414_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2465611.1"
                     /translation="MENILEVYNLNKTYTDFSLSNISFALPEDCITGFIGVNGAGKTT
                     TIRSILDVVKRDSGKIKIFGKDFDGNEKELKNRIGVVFDDSYFYEELSMAEMKSIIAP
                     SYDNWCEEDYQQNMKRFNLNPKQKIDTLSKGMKMKFALALALSHKAEFLIMDEPTSGL
                     DPLIRSQILDILTGYMKKCGRGVFFSTHITSDLDKIADMIVMIDNGRIILEREKDTLL
                     DEHRKVKGDIQFLNDEIKKLFLNLEITGYGFIGITSKINEVQKNMPDILIERPTIEDI
                     MLAYIERRE"
     gene            complement(3933..4307)
                     /locus_tag="PC414_003239"
     CDS             complement(3933..4307)
                     /locus_tag="PC414_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2465612.1"
                     /translation="MKVLISNTSDKPLYQQIKEQIKEAIFTEELKEGDAMPSIRNFAN
                     DLKVSILTIRRVYEELENEGFLTRQVGIGTFISTGNLEILRDSKRRIVEQKMADMIKD
                     AKALGITQIELQEMMDILYQED"
     gene            complement(5469..6659)
                     /locus_tag="PC414_003240"
     CDS             complement(5469..6659)
                     /locus_tag="PC414_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDF2465613.1"
                     /translation="MSDMLLWFSVVLVLFAIGDLIASKTKAKVSAVFVTLLLFLILFV
                     TKAIPADIIEKAGMTAAASWSVPMIMFSMGTMLNVKQFIDEWRTVLTAWLGIVAVIVC
                     VSLCIPLFGKSTVLTSIPVINGALPATTIMTQAALEKGLTLAAATATVVFAIQKFIGT
                     PIASRAALQEANRLLVEYHEAKSKGIDLANVDTDKKEAENTGTKVKQAFCEKYDKYYS
                     TNVCIFFIALFSYLGYELSEIIHVNYSIVCLVVGVIVTRIGIVPKDILEKGKIKGFIN
                     MVVFAAVIPSLAKVSLTDLISLFVPIVGMFAASIIGIFLMMKVLPGWKIIGSKPLAFG
                     VGFCQMLGFPTTYLISNEVCNAVGETEEERAYLMSKIMPKLVVGGMACMISIVVAGLM
                     VPML"
     gene            complement(6675..8630)
                     /locus_tag="PC414_003241"
     CDS             complement(6675..8630)
                     /locus_tag="PC414_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HDF2465614.1"
                     /translation="MEKIKIESSDIFEYIFPHDISCSPDGKHIAYIISNINEEKDCYE
                     HDLYVMDIKTEKQIHMTQTKDVTSFSWISNTELLFTSKRNKPKAGTTDFYTISIEGGE
                     AKKAFSIPKACSVPVSLGNKLWLLTTKNPTDSKKSEPDRAVEGVDYWTFTDKPFIRDG
                     ENFSQRRRVTLELYQEGENITKAITPKFCEVAGIDVSSDKNRILYTGQIYEDCATPFS
                     GLWEYHIDSGETKELVPQGKYQISLAKYIGKDKVMLQASTLDRSITQNHDIFILDLST
                     SEINMIASPDGMYATLLDVDAVYGGGRSNKVIGDKFIGARICRTMTEFNEFDTKTGNI
                     RIITKVDAFTSFDIYDNTMYTVMLKDYELAEIYSIDMTTGTMKKMTAFSKPYLDTHKV
                     SLPEKLTFVAKNKEEVDGFVIPPIDAKEGEKYPAVLFIHGGPKWAYGYMFTHLKQCVT
                     SKGMYVIYCNPHGGDGYGEKFLEMVERWGYVDYEHLMEFVDTCIEKYPGIDADRLGVA
                     GGSYGGYMTNWIIGHTDRFKAAVSQRGISNLITASLIIDFGDRIMKQTCGDKTPWNHE
                     EVLWNHSPIKYVKNVKTPTLFLHSDRDYRCFMGDTFQMFTALKQLGVDTEMYLFHGDT
                     HGLSRNGRPSNRIARANAIVDWFERYL"
     gene            complement(8647..10713)
                     /locus_tag="PC414_003242"
     CDS             complement(8647..10713)
                     /locus_tag="PC414_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HDF2465615.1"
                     /translation="MKEVVGSLKKITKERIENTMKKLFTSETFLEYQFISDCQISPDG
                     GYTAFIVKKADIKENGYTSQVYILNNKTGELKQITSINSVGAYAWEDENTILFPALRN
                     EKVKEAVKNGKQCMSYYALSLNGGEAEELFRLPIKGGKLNPIGKGLYAVIDSYDNDRP
                     IVEGLPEEEQQKKINAYNKRHYEHFKEIPYAVNGEGYISRKRKRLYVYDSNINELKAI
                     TAPMFNVVGMKISDGKILYIGQEFKDVKGLKNGVYVFDTNTNTNVCILEKDKYIIKGF
                     ELYQNQVILNLTDALSYGNGENGDFYTIDIDTKEMKLLSGHQHHCIGNTVTSDVKMGA
                     GQTTKVDGEYIYYTSTVDMDCIIERIHIPTGKQEKVTQTGSVDFIDVKDGNIVCVGCL
                     GNGLPEVYTVENGTLCKKTHLNDHILEEYKISVPEYIESKGSSKWEIQGYVYKPVDYE
                     VGKKYPAVLAIHGGPRLTYGPYFMHEIQVFTSAGYFVFFCNPRGSEGRGNAFADIRKQ
                     FGEIDYIDFMEFTDTVLEKYTDIDKTKLAVEGGSYGGFMTNWIIGHTNRFAVACAQRS
                     IANWSGMEGTTDIGYYFCKGQTGASHMENHELQWKQSPLAYVDKCVTPTLFLHGEKDY
                     RCYMQEAFQMFSALKIHGCPTKLCLFEGENHELSRSGRPKQKLQRLVEMLDWFSVYIK
                     KDNIGQ"
BASE COUNT         3695 a         1900 c         1243 g         4074 t
ORIGIN      
        1 tttactcact ctacattatg atatattatt taaatatttt tcttcactct gcttatatta
       61 tgatacataa tttaaacatt tttcttcact ttgcattatg atatatggtt taaataatct
      121 tctttacttt gtattatgat acatgcttta aatattttta cgcactttat attataatac
      181 atgctttaaa taatcttatt cgctttgtat tataacacat tatttaaata tttttactca
      241 ctctgcatta tagtacatta tttaaatatt tttcttcgct ttgcattatg atacattatt
      301 taaatatttt tactcacctt gtattatggt acattatcta aatatttttc ttcactctgt
      361 attatgatat ataacttaaa tattttacct cactctacaa tattatatat aatttaaata
      421 tttttcttca ctttgtatac taaagtttat ttaaagtatt ttctttccca ttatactatg
      481 ctgttaaata tttttcaact gaagtattgt agaaccttaa aattactata aaaaactata
      541 ttctaaaata cttgtaatac tacgtatcac ccttaacact taagtataat tttaaagcaa
      601 aattcttatt acttatattt aattttgatt ctacatcttt agatataatt ttaagtaatt
      661 taagaaatta ttgtaaatca tatagtacta ctcaataatt aggtttttca atgttccaat
      721 tatgtttctt tgatataatc caccatgact aaaaactatc gtttctatat cataattact
      781 tatattttta atagcatctt ttaactcttg tttgttgctg tgcattacat caacaggctt
      841 aagttctcca ttttctacct gtaacaaatc tccagcaata agtactttag actcttttaa
      901 ataaagacat atgtgacctc tagtgtgacc tcctgtatgt ataacttcta tttcctcacc
      961 tagattcaat ttttcatggt tatctaatga cacatctact ttagtatatg atgatttaaa
     1021 tccaagtccc ataacattta gcatactaag catcttatca tcaattttat ctaccatttg
     1081 ttccaacata taaagtttaa aaggagtttc ttctcctgtt atatagctga tttcactttt
     1141 gtatgcatat actttgatat tattcttaga tttttctctt agataattta tgtttcctat
     1201 atggtctata tcatgatgag taattattat attttttaac ttatcaaagc tcgtgttact
     1261 tttattaaga gcatcaatta ttaaatcacc ttgacccgga agacctgtgt caataagtgt
     1321 tgcatcatct tctgtgtaga ttacaggaat atacataact ttatcttcac cgaatacatt
     1381 tgatgaaatt tttaaaacat ctaatccctt tgctaaattc ataatttttc tccttataat
     1441 tacttaatta gaggtcaatt taattaaaga aattgagaaa atgtagattt tcctctagtt
     1501 atattttatt aattaaaaat tattaatacc tagtatatca cttaagttga aggattactc
     1561 gacttgattt ttttgttatt ttatggtata gtaattaata tagaagtaag taattgcaag
     1621 ctttgcaatt acttttattt ttttctaata actattttat ttttgataat tcgtataaaa
     1681 ttaaaagtaa agtaatatgg aaaatatgaa tatagaaaaa ctttaataag aaaataaatt
     1741 atgaataaat aaaagaaacg caatactgta tatagatgat tatttagaaa aataaaatct
     1801 cttttaataa aaataagctt caaaataaac ttttagttca taaagcttag gaggaactaa
     1861 attgagtgca agtgtatcta ctgttcaaaa taaatagttc ataaagcact cttttttata
     1921 ttaaatcaat tatattttcg tcatttcagc aatgaaaaag aaacttatct ctattttgag
     1981 acagcttctt tttattaact tcctactttt tattagtttt ctaaaattga aaagctttat
     2041 ttataggatt ccttagattt atattttgac gatataattt tttaaaaaca tcttaaaaga
     2101 tggtatacaa tacacacaat catactcata attgaaaaat aatcaatgga aaataaaatt
     2161 actggatatt atctattaaa aaatacaaat tgattcacaa gaaacatata atctataata
     2221 ttatgatgtt actctctcgc accaagtaac tttattttca agtaaccttt taaattcata
     2281 ccatctaact tcactcaata aaaatggcaa tataaatatg tactctattt tttactttta
     2341 aatatctgac aagctttata tactattgga agtaaaatac aaagtaaaac agcccaatct
     2401 ggtaaatgtg gcatataaaa gtccccctta ccttatacaa gctccttatt actaaaaatt
     2461 ttaatagaaa taaacattga agctaacagt ataattaaac ttaaaaggaa caatacaata
     2521 ttcactatca tggatggata ctgatatata aatggaattt caaaattgct gtgagctata
     2581 gttaatggta atacaaatga tgaagccatg attatcacca cgaaaaagaa ttttgttgtt
     2641 tcatatccta atttatattg aataggcata taaactccat aaataataga acttattaat
     2701 aaaacaatca aaatatatgt taagtcaaaa gttcctattt gtggaataag taaattttca
     2761 atccagtaaa ctaatgtaca atagataaat accattgcaa ataaaacata cttagattga
     2821 actaagccac gtctaggata aggagttgaa caaagtaata cagatgcttt tgaatattga
     2881 ctctccttta ataatagata ctggcacagc ataaattctg aaaatataat ggatagtaca
     2941 aatcccaata tttgtgcaaa tggaggtaaa cgccacaatg taaataatgg gattcctatt
     3001 gcaataaaca atatcaacaa tacatatttt tttacaatca gaatatcttt tttaactaaa
     3061 tgaatcaaca taattactct ctcctttcaa tatatgcaag cataatatct tcaattgtag
     3121 gacgctctat taaaatatct ggcatatttt tttgcacttc attaattttt gaggtaatcc
     3181 ctataaaacc ataacctgta atttctaaat ttaaaaataa ttttttgatt tcatcattta
     3241 gaaattgaat atcccctttt acttttctat gttcgtccaa tagagtatct ttttcacgct
     3301 ctaatataat acgtccattg tcaatcataa caatcatatc tgctattttg tctaaatcag
     3361 atgttatatg agtggaaaag aaaacaccac gaccacattt tttcatatat cctgttagta
     3421 tgtcaagtat ctgactacgg attagtgggt ctaaaccact tgtaggttca tccataatta
     3481 aaaactcagc tttatgcgat aatgctaatg ctaatgcaaa tttcattttc attccttttg
     3541 aaagtgtatc aatcttttgt tttgggttaa gattaaaacg cttcatattt tgttgataat
     3601 cttcttcaca ccaattatca tatgaaggtg caataatact tttcatttct gccattgaca
     3661 actcctcata gaaatatgaa tcatcaaata caactccaat tcgatttttt aattccttct
     3721 catttccatc gaaatcttta ccaaagattt ttatttttcc actatcacgc tttacgacat
     3781 ccaatatcga acggattgtt gttgtttttc ctgctccatt aacacctata aatccagtta
     3841 tacaatcttc tggtaatgca aagcttatat tagataatga aaaatcggta taagtcttat
     3901 ttaagttata aacttctaaa atattttcca tattaatcct cctgatatag aatatccatc
     3961 atttcttgta actctatctg tgtaattcct agtgcttttg catccttaat catatctgcc
     4021 attttttgtt caactatacg tctctttgag tctcttagta tttctaaatt tccagttgaa
     4081 ataaatgttc ctattccaac ttggcgtgta agaaagcctt cattttcaag ttcttcatac
     4141 actcgtctaa tagtcaaaat actaactttt aaatcattag caaaatttct tatggatggc
     4201 attgcatccc cttctttcag ttcttcagta aatattgctt ctttaatttg ctctttaatt
     4261 tgttggtata atggcttatc tgatgtgtta gaaatgagca ctttcatttt ttatcacccc
     4321 ctttttatca ttttcagtgt gatgttgtta tatacacact atacacttag agcacttttt
     4381 tgtcaatata aaagatgtaa aatattacta tgacaaaata aatatgaatg aaatattaga
     4441 gattgttgat agctataaac actttttaaa accacataag atagaatgta ttaaatattt
     4501 attaaaagta agaattgaat atctaaaaaa aagaggaatc ctgtatgaat gatttgtttg
     4561 atgataaatt aagttataaa ctatttataa aaaacataaa ggaataaatc catttctaat
     4621 tacaaacata ctaaaaaatt tagatagtaa taaatagata gtaataaata gatagtaata
     4681 aatgggatga atttataaga atttggatag aggaaagagt tattcaagag tgtagactag
     4741 aggtaaactc tatattaaaa aaatttaaat ttggatatta tgatgcatgg aatatttgca
     4801 gaagtaacct tgctataagt atagaagatt tttttgatta caaaagataa tactctaaaa
     4861 tgtgaggatt tcaacattca atactgtact ttaaataata tcactgcaaa ataccatcca
     4921 ccttttacca taattgaata ccctaattat tttaaaaaag aaaacattag aaacagaaaa
     4981 ccttggatat ataaaataaa aaatactaac ttctaaaaca cggatactct ttgataaata
     5041 aaacaaaaaa taattttaat aattaaaatt ggctatctca aactagagat aagtctcttt
     5101 ctcactttta aaacgttgaa aatatattcg atttcgtaca aaaaagagtg ccttagaact
     5161 aaaaagttca ctttgtggca ctcccttttt tctcctcttt ttaatttttt ataaaaattc
     5221 ttcaaaaata ttattaaata ccctatatta tacataaatc aaattaaagt tgcattttag
     5281 caaataaaaa caaattataa ctattagcat agcaaataaa aaatctgctc tcttataaaa
     5341 tcaaattata attataaaaa gaaaatattc ttataacaaa ttagctaata taaaattatt
     5401 ttataaatta atactacttt ttagcttctc ctataaatta ataccacttt tttaactttg
     5461 aaacctatct acaacattgg taccataaga ccagctacca caatagaaat catacaagcc
     5521 ataccaccaa ctaccagctt aggcataatc ttagacatca aatatgctct ttcttcttcc
     5581 gtttctccaa cagcattaca cacttcattt gaaattaaat atgtagttgg gaatcccaac
     5641 atctgacaaa accctactcc aaaagcaaga ggtttagaac cgataatttt ccaacctggt
     5701 aacaccttca tcataaggaa tataccaatg atagaagctg caaacatacc cacaattggt
     5761 acaaaaagac taattaaatc tgtcaaagaa acttttgcta aagaaggaat aacagctgca
     5821 aacacaacca tgttgataaa tcccttgatt tttccttttt ccaaaatgtc ttttggcaca
     5881 ataccaatac gtgttacaat aacaccaaca acaagacata caatagaata atttacatga
     5941 ataatctctg aaagttcata ccctagataa gagaataatg ctataaaaaa tatacaaaca
     6001 tttgtagaat aatatttatc gtatttttca cagaaagctt gttttacttt tgtgcctgta
     6061 ttctctgctt ctttcttatc agtatcaaca tttgctaaat caatgccttt tgattttgct
     6121 tcatgatatt ctactaaaag acggtttgcc tcttgtaacg ctgctcttga agctattggt
     6181 gtaccaataa acttctgaat tgcaaataca actgttgctg tagctgctgc aagtgtcagt
     6241 cctttttcta gtgctgcttg tgtcataata gtagtagcag gtaaagctcc atttataact
     6301 ggtatgcttg tcaatactgt tgattttcca aacaaaggaa tacataatga gacacagact
     6361 ataactgcta caattccaag ccatgctgtc aatactgttc tccattcgtc tataaattgt
     6421 ttaacattca acattgttcc catactaaac ataatcattg gaacactcca actagctgct
     6481 gcggtcatac ctgctttttc aataatatca gctggtattg cttttgtgac aaatagaatc
     6541 aaaaataaaa gtagtgtaac aaatacagct gatacttttg cttttgtttt gcttgcaatt
     6601 aaatccccaa tagcaaataa aactaatacg acactaaacc ataataacat atcactcata
     6661 gaataacccc cttattataa atatctttca aaccaatcta caatagcatt tgctcttgca
     6721 attctattag atggtcttcc atttctagac aatccatgtg tatctccgtg gaacaaatac
     6781 atttctgtat ctacaccaag ctgttttaat gctgtaaaca tttggaaagt atcccccata
     6841 aaacatctat aatccctatc actatgtaag aacaatgttg gtgtcttaac atttttcaca
     6901 tatttaatag gtgaatgatt ccaaagaacc tcctcatgat tccaaggcgt cttatctcca
     6961 caagtctgct tcataattct atccccaaag tcaataatta aagaagctgt aatcaagtta
     7021 ctaatacctc tttgagaaac tgctgctttg aaacggtctg tatgaccaat tatccagttt
     7081 gtcatataac caccgtaact tccccctgca acccctaagc ggtctgcatc aatcccagga
     7141 tatttttcaa tacaagtatc tacaaattcc ataagatgct catagtcaac atacccccat
     7201 ctttctacca tttctaagaa tttttcacca taaccgtcac caccatgagg gttacaataa
     7261 atcacataca ttccctttga tgttacacac tgcttcaagt gtgtaaacat atagccatat
     7321 gcccatttag gcccaccatg aatgaacaat acagcaggat atttttcgcc ttctttagca
     7381 tctataggtg gtataacaaa accatcaact tcttctttat tttttgctac aaacgtcaat
     7441 ttttcaggta atgaaacctt atgagtatct aaatatggtt tagaaaaagc agtcattttt
     7501 ttcattgtac ctgtagtcat atcaatgcta tatatttctg ctaactcata atctttcaac
     7561 attactgtgt acatggtgtt gtcgtaaata tcaaaagatg taaaagcatc tacttttgtg
     7621 atgatacgta tattacctgt ctttgtatca aactcattaa attctgtcat agttctgcaa
     7681 attcttgcac caataaattt atctcctata actttattac tacgtccacc accatatact
     7741 gcatctacat ccagtaatgt agcatacata ccatctggac tagcaatcat atttatttca
     7801 ctagtactca aatcaagtat aaaaatatca tgattctgtg taatagaacg gtctaatgtt
     7861 gaagcttgca acattacttt atctttgcca atatattttg ccaaacttat ttggtacttt
     7921 ccttgtggta caagttcttt ggtttcacca ctgtctatat gatactccca aagtccagaa
     7981 aaaggagttg cacaatcttc ataaatctga ccagtatata aaattctgtt tttatccgaa
     8041 gaaacatcta tacctgctac ttcacaaaat tttggtgtaa tggcttttgt tatattttca
     8101 ccttcttgat ataattctaa tgttacacgt cttctttgag aaaaattttc tccatctctt
     8161 ataaaaggtt tatctgtaaa tgtccaataa tctacaccct ctactgccct atctggttct
     8221 gattttttag aatccgttgg attttttgtt gtcagtaacc acaatttatt tccaagtgat
     8281 acaggtacgc tacaagcttt tggaatagaa aatgcttttt ttgcttctcc tccttctata
     8341 gaaatagtgt aaaaatctgt tgtacctgct tttggcttat ttcttttaga tgtaaaaagt
     8401 agttctgtat tagaaatcca actaaatgat gttacatctt ttgtttgagt catatgtatt
     8461 tgtttttctg ttttgatgtc catcacatat aagtcatgtt catagcagtc tttttcctcg
     8521 ttgatatttg atataatata agcaatatgc tttccatcag gtgaacaaga aatatcatga
     8581 gggaatatat attcaaaaat atctgaactt tcaatcttta ttttttccat ttttatattc
     8641 tcctttctac tgtcctatgt tatctttttt tatatatacc gaaaaccaat ctagcatttc
     8701 aactaatctt tgtagctttt gttttggtct accagaacga gacagttcat ggttttctcc
     8761 ttcaaataga caaagttttg taggacaccc atgaattttc aatgctgaaa acatctggaa
     8821 tgcttcttgc atatagcatc tatagtcttt ttcaccatgt aaaaataaag tcggtgtaac
     8881 acatttatca acataagcaa gaggtgactg tttccattga agctcgtggt tttccatatg
     8941 agaagcacct gtttgtcctt tgcagaaata ataaccaatg tctgttgtac cctccatgcc
     9001 actccagttt gcaatagaac gctgagcaca agcaacagca aaacggtttg tatgaccaat
     9061 tatccaattc gtcataaatc caccataact tccaccttca acagccaatt ttgttttatc
     9121 aatgtctgta tatttttcaa gtacagtatc tgtaaattcc ataaaatcta tataatcaat
     9181 ttctccaaac tgtttcctaa tatctgcaaa agcattgcca cgaccttcag acccccttgg
     9241 gttacagaag aatacaaagt aacctgcact tgtaaatacc tgtatctcat gcataaaata
     9301 tggtccatat gtcaatcttg gtcctccatg aatcgccaat acagctggat attttttccc
     9361 cacctcataa tctactggtt tatatacgta tccttgtatt tcccatttag aacttccttt
     9421 tgactctatg tattctggca cagaaatttt atattcctct aaaatatggt catttaaatg
     9481 tgttttctta cacaatgttc cattctctac tgtataaact tctggtaatc catttcctaa
     9541 acaacccaca catacaatat tgccatcttt tacatctata aaatcaactg aacctgtctg
     9601 tgttactttt tcctgttttc ctgttggaat atgtattctt tcaataatgc agtccatatc
     9661 tactgtagaa gtgtaataaa tatattctcc atctaccttt gttgtctgac cagcacccat
     9721 tttaacatca gatgttactg tatttccaat acaatgatgt tgatgccctg aaagaagttt
     9781 catttctttc gtatcaatat caatagtata aaaatctcca ttttctccat ttccataaga
     9841 taaagcatca gttaggttta gtatcacttg attttgatat aattcaaaac cttttataat
     9901 atacttatct ttttctaata tacagacatt tgtgtttgtg tttgtatcaa atacatagac
     9961 cccatttttc aaacctttta catctttaaa ttcttgccct atatataaga tttttccatc
    10021 acttattttc atacctacaa cattaaacat aggtgctgta attgccttca actcatttat
    10081 attactatcg tatacatata ggcgttttct ttttctgcta atatatccct ccccattgac
    10141 agcataagga atttctttga aatgttcata atgtcttttg ttataagcat ttattttttt
    10201 ctgttgttct tcttctggca atccctctac aataggtcgg tcattgtcat aagaatcaat
    10261 aactgcatac aaaccttttc ctataggatt caatttacca cctttgatag ggagtctaaa
    10321 aagttcttct gcttctccac catttaaaga taaagcatag taactcatgc attgcttacc
    10381 gtttttgaca gcttctttta ctttttcatt tcgcaaagca gggaacaaga tagtattttc
    10441 atcttcccag gcatatgctc caacagagtt tatagacgtt atttgtttta attcgccagt
    10501 tttattgttt aatatataaa cttggcttgt gtaaccattc tctttgatat cagctttttt
    10561 tacaataaat gctgtatatc ctccatctgg tgatatttgg caatcactta taaattgata
    10621 ttccaaaaat gtttcactcg taaatagctt tttcatagta ttttctatcc tttctttagt
    10681 aatttttttc aaactcccta ccacctcttt cattttttat tttaaattta aaataaaaaa
    10741 ttttaaattt aaaataaaaa attttataaa ggttattttt ttaggaagcc ttctggcttc
    10801 cttgtaatat ttgtaattta aagtatattt aatccatgct taattcatgc ttaattcata
    10861 cttaatctat acttaatcta tacttaatct atacttagtc catacttaat ct
//
LOCUS       DAMJVK010000116        10886 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.121, whole genome shotgun sequence.
ACCESSION   DAMJVK010000116 DAMJVK010000000
VERSION     DAMJVK010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10886
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.121"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(293..1342)
                     /locus_tag="PC414_003243"
     CDS             complement(293..1342)
                     /locus_tag="PC414_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465616.1"
                     /translation="MVMQVQEKILELLYTINEDVVSIKNDMSEVKQEINVLKEDMSEV
                     KQEMTVMKEDMSEVKQEINVLKEDMSEMKQEMTVMKEDMSEVKQEINVLKGDMSEVKQ
                     EMIVMKEDMSEVRQEINIMKEDMSEVRQEINTMKEDMSEVRQEMTVMKEDMSEVKQEI
                     NVLKEDMSEVRQEINIMKEDMSEVRQEMTVMKEDTSEVKQEINVMKKDMSEVRQEITV
                     MKEDVSEVKSEVNFMQNKINNINEDMNGIKDEVSIANEKLYGIEIKIDSLECEDKSMK
                     EIQIEQNNILGSLLHNSEINKATHDNIEHNIAYIKGDTNSIKEDIAEIRRDLNLVELA
                     TSKNWSDIVKLKSVK"
     gene            complement(1572..2657)
                     /locus_tag="PC414_003244"
     CDS             complement(1572..2657)
                     /locus_tag="PC414_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HDF2465617.1"
                     /translation="MYNFLGKELKSPLIIGSGPLTYSAAGCKILSDAGAGAVVTKTIR
                     KERAINPAPHMVRNTANALLNNEKWTDFEPEQWIDFEIPQMKRDGTVCIASIGHTIEE
                     SSELVEKVANAGADFIELVSYDYRDLIPMLKDAKERVNIPVIVKLPPMIDEIGDFAKK
                     LEEAGADAITACDSVGPAFRIDIETGQPLLGGNGIGYLSGETIKPITLQRIYEIKKQV
                     NIPIIGLGGCVSGDDALEMIMAGADFVGICSVVILKGAQVISKIHDDLKSNLNRLGYN
                     TIENACGIVHNHMGDVNERLSFEFTYDEEKCTKCKMCERVCAYQARKLNDKMELNEEE
                     CRYCGLCISVCKPKALGRKISCSELNV"
     gene            complement(2693..4006)
                     /locus_tag="PC414_003245"
     CDS             complement(2693..4006)
                     /locus_tag="PC414_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDF2465618.1"
                     /translation="MIAIKSKYLISSADEIYTNSAVVVENDIIKDILPNEEVEIKYKN
                     IEEIIDKSDAIIMPGFVNGHMHQYGVLSRGIPANVHFTDFEGFLNDYWWPFIENRIGL
                     KEVKATTKASAIELIESGVVAFCDTLEAPNTEEGTLIEQGKILEEIGMKAILSLESCE
                     RISFENGLRCLDENSNLIKWSRENSKLINGIMCTHTSFTCSDRFIKKAKEDAKKLNAP
                     MQFHLCESIYEPNYAEKHFGKKAVDYYNDLDILDETVLASQCVKVNDEEIDILKEKGV
                     KVVHMPLSNCEVGGGFSPVPKMIKKGIKVALGTDGYINDFFEVMRGAFLMHKSVEEDA
                     SVMPANLVFRMATEHGAYVLGLQNSGKIAVGNKADIIVMEDEFKTPVTLDNIFDQIVV
                     QGKKEFISNVYIDGRHILKEKQLVDLDKKAIVKEMKEVACEFWKF"
     gene            complement(4007..6298)
                     /locus_tag="PC414_003246"
     CDS             complement(4007..6298)
                     /locus_tag="PC414_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF2465619.1"
                     /translation="MVNKEVRKIDSEAIVTGKPIYTEDLIFHKDVLTIKLLRSPHAFA
                     KIKNIDIKNALKVKGVVNIFTYKDVPNYRYSMVGESYPEASPHDQLILEDVVRYVGDE
                     VAIIAAEDERAAIKAMKLIKVEYEVMTPILDARESEGNKILIHEEDDLFCPFDFGLDA
                     TKNIVSREKMGKGDVEKEFKESDVIVEHSYMTQSQAHCMMETHRAYTYIDTNGRLVVT
                     SANQSAYHMRRQVSKALGIPLSKVRVIKPRIGGGFGGKNIAVTEIYASFVTWMTKRPA
                     KLIYTREETFAMTNTRHQMFFDIKIGADKEGNIKAIDMKALNNTGAYGDNGPSVCSES
                     GHNVLPTYNNVPAIKFDGKTVYTNLVPGGALRGYGATQGTFALDCAIDELAHKLNMDP
                     TEIKLKNIIKKQTEGGIMNFPIRSCALDECIIRGKKLIGWDEKYPVRDIGNNKVRAVG
                     MSVGTHGSGIANIDMAIVNMRMDEDGTYKLFSGSSDLGTGSDTILAQIAAKALNTSID
                     RISVYTGDTDMCPYDTGAYASCTTYVTGNATIKSAESLKQKILKVAQKKLDLPIDDLI
                     LYEDRVCYSEDVEKFVLLSELGKESVGGGNQEVLYSSESYGIQESAKPFLAGFAEIEL
                     DKTTGEFKVINYACSTDCGTVMNPALARIQVEGGVTQGIGLAMYEDPRHGSDGKLQTS
                     NLLQYKVPTKKDIGNIIVDFVDNYEPTGPYGAKSLGEIVIHTPAPAIANAIYNATGVR
                     IRELPITSEKVYMAMKKLNQGGK"
     gene            complement(6288..7082)
                     /locus_tag="PC414_003247"
     CDS             complement(6288..7082)
                     /locus_tag="PC414_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HDF2465620.1"
                     /translation="MITIKEYVVPKSLDEAYELLISRKNNIILGGCGFLKLGSKNIGS
                     AIDLKDLALDYINETDDSILIGADTSLRTLELNKVIKNYCNGVISNAVSNIVGVQFRS
                     GARVGASVFAKYGFSDLIPSLLVVDAKVKLYKKGVMNLADFLENELEKDILIEVILPK
                     KDAIGVFDSIRKCTGDFAVLNGAMLKEGSIYKIAIGARPRRAKIAYKASEILSLENDI
                     EKAGEVSSEELSFGSNIRGSKEYRKDMTKALVVRMYNSIGGECDGK"
     gene            complement(7121..7567)
                     /locus_tag="PC414_003248"
     CDS             complement(7121..7567)
                     /locus_tag="PC414_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HDF2465621.1"
                     /translation="MIIKCTINNKEKELSIRPDDFLADTLRENGYVSVKKGCDTGSCG
                     LCTVMVNDKAVLSCNYLTARVEGQHITTLEGVLDEAKKFGDFLADEGADQCGFCAPGF
                     IMMVLAMKRELQNPTEEEVIDYVNGNLCRCTGYKGQLRAIHKYLNT"
     gene            complement(7639..9045)
                     /locus_tag="PC414_003249"
     CDS             complement(7639..9045)
                     /locus_tag="PC414_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2465622.1"
                     /translation="MMDILIKNAIIVTVNKEREVIFDGALVVKDNKIADIGNSKEIES
                     KYTDVKKIIDAKGKVLFPGFINTHNHLFQTLLKGLGDDMVLKDWLETMTFPAANYLEP
                     KDTYDAAMLGCIEGLRSGITTMVDYMYPHSKPGLCDGIIDAYKELGIRGILGRGCMNT
                     GAQFGVHPGIMQDVETVEKDVRRLFEKHHNTENGRIKIGVAPAAIWSNSQEMLEMLWR
                     VVKEYDDALFTVHISETPFDREAAKELHGQYDIDVLEKLGILGPNVLMVHCVYLTEKD
                     MELTKKYDMKVSHNTASNMYLSSGVAPVPEMLKKGITVSLGVDGAASNNSQDMLELMK
                     LTALQHKVNKCDPLAMSAEKVLELATIDGARAIGMEDEIGSLEIGKKADLLIFNPMLS
                     PKAIPMHNPVSTLVYSSSMKNIESVIVDGNIIMEDSKILTANEEKALKDAQDTAERLC
                     IRGTIKNRMEGHKWNSLY"
     gene            complement(9063..10433)
                     /gene="hydA"
                     /locus_tag="PC414_003250"
     CDS             complement(9063..10433)
                     /gene="hydA"
                     /locus_tag="PC414_003250"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HDF2465623.1"
                     /translation="MGTILRGGTIITSDKTYISDLRIEDEKIVEIGNNLEINGDKVID
                     ATGKIVIPGGIDTHTHFDMNAGSITTADNFETGTRAAIAGGTTTILDFAEANEGENLL
                     QGVEAYHKKASGNCYCDYGFHMTITCLNKDTFNHMEKLISDGIVSFKMYMAYDGMKVD
                     DGTIYRVLKKARDLGCIVGFHCENGDLLDVLIEENIQNGNLEPKYHPLTRPNIVEKES
                     VSRLADITKLSNSKSYVVHLSCRESLETVKIAREKNIDMIVETCPQYLLLEDNLYNKD
                     KFEGAKYVMSPPLRKKEDINYLWEGLSSGDIQTVGTDHCSFNFKGQKDLGIDDFSKIP
                     NGAPGVEHRLALLYTYGVLENRISVNKFVEVTSTNAAKIFGMYPKKGEIAVGSDADIV
                     ILNINKEETISYKTQKQNVDYTPYEGFKVKCKVEDVFLRGNHVVQSCNVKEHPTGQYI
                     KRKITK"
     gene            complement(10566..>10886)
                     /locus_tag="PC414_003251"
     CDS             complement(10566..>10886)
                     /locus_tag="PC414_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDF2465624.1"
                     /translation="VEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYKGLKEAGIDS
                     EITQYSFCTNGSHYAGEAGIKTIGFGPSKENLAHTIDEYIEQEQLFIGTEGYYGILKS
                     VYGK"
BASE COUNT         3160 a         2194 c         1135 g         4397 t
ORIGIN      
        1 attttagtgc caatatttgc atacatttgt atagtataaa atttttatgt tctcattgat
       61 aaaattttac ttatttcaag cgttctatta gattttatca tctaaatttt aggttttatt
      121 tttataaatt aattgtattt acataaaaaa aaagacctaa aaacaaattt ttaacggtct
      181 tttctcaatt ttttattttc atttctaaaa tttataataa atagtaagaa cactataaat
      241 tcttttatat aaaattctta gtttgtggat gataaattag tgaattaaaa tactacttta
      301 ctgattttag tttgacaata tcactccaat ttttactagt ggctaactct actaaattta
      361 agtctcttct tatttcagct atatcctctt ttatagaatt agtgtcacct tttatatatg
      421 ctatgttatg ttctatatta tcatgagtag ctttatttat ttcagaatta tgtaataaac
      481 ttcctaaaat attattctgt tctatctgta tctctttcat tgatttatct tcacattcta
      541 gactatcaat cttaatttca attccatata atttttcatt tgctatggat acctcgtcct
      601 taattccatt catatcttca ttaatgttat ttattttatt ttgcataaag tttacttctg
      661 atttaacttc ggaaacatct tctttcatta cagttatttc ttgtctcact tctgacatgt
      721 cttttttcat tacatttatc tcttgtttta cctctgatgt atcttctttc attacggtca
      781 tttcttgtct tacttcggac atatcttctt tcattatatt tatctcttgt cttacttcgg
      841 acatatcttc ttttaataca tttatctcct gttttacttc cgacatatct tctttcatta
      901 cagtcatttc ttgtcttact tccgacatat cctctttcat tgtatttatc tcttgtctta
      961 cctcggacat atcctctttc attatattta tctcttgtct tacctcggac atatcttctt
     1021 tcattacaat catttcctgt tttacttctg acatatctcc ttttaataca tttatttcct
     1081 gttttacttc cgacatatct tctttcatta cagtcatttc ttgtttcatt tccgacatat
     1141 cttcttttaa tacatttatt tcctgtttta cttccgacat gtcttctttc attacagtca
     1201 tttcttgttt tacttccgac atatcttctt ttaacacatt tatttcttgt tttacttccg
     1261 acatatcatt ttttatgcta actacatctt catttatagt atataataac tctaaaatct
     1321 tctcttgcac ttgcatcacc atccctatta attattataa cattttacac aaatgctata
     1381 caatgttaac atatatatca gaattattaa aattttaatt aaacaaaatt gattcacttt
     1441 attacttcaa aactaattaa tatctaaaat aactacaaat aaacaaaaat aaggtcaaca
     1501 acaaaaaagg cttatctctt tgagatagcc tttcttattt ttatttaaaa ctttgttttt
     1561 tataaaataa tctatacatt cagctctgaa catgaaattt ttcttccaag tgcttttggt
     1621 ttacaaactg atatacaaag tccacaatat ctacactctt cttcattcaa ttccatctta
     1681 tcattcaatt ttctcgcttg atatgcacaa actctctcgc acatcttaca tttagtacat
     1741 ttttcttcat cataagtaaa ctcaaagcta agtctttcat taacatctcc catatgatta
     1801 tgtactatac cacaagcatt ttctatagta ttatatccta atctattaag attagatttt
     1861 aaatcatcat gtattttaga tattacttgt gcacctttta aaattactac tgaacatata
     1921 ccaacaaagt cagcacctgc cataatcatt tctaaggcat catctccact tacacatcca
     1981 cctaagccaa ttattggtat attaacttgt ttttttattt catatattct ctgtaaagtt
     2041 attggcttga tagtttctcc acttaaatat cctattccat ttccacctaa taaaggttgc
     2101 cctgtttcta tgtctattct aaatgctggt cctacagagt cacaagcagt tattgcatct
     2161 gctcctgctt cttctaattt tttagcaaaa tcgcctatct catctatcat aggaggaagt
     2221 ttaactatta caggtatatt tactctttcc tttgcatctt taagcattgg tatcaaatct
     2281 ctatagtcat aagatactaa ttcaataaag tcagctcctg catttgcaac tttctctact
     2341 aattcgctac tttcttcaat tgtatgacct attgaagcta tacagacagt cccatctctt
     2401 ttcatttgtg gtatttcaaa atctatccat tgctctggct caaaatctgt ccatttttca
     2461 ttatttaaaa gagcattagc tgtatttcta accatatgtg gtgctggatt tatagctctt
     2521 tcttttctta tagtcttagt cacaactgcc cctgctcctg catcacttaa tatcttgcat
     2581 ccagctgcac tatatgttaa tggtcctgaa cctattataa gtggtgattt taattcttta
     2641 cctaaaaaat tatacattat ctgcttcccc ttttcatata tatttacatt cgttaaaatt
     2701 tccaaaattc acaagcaact tctttcattt ctttaacaat agctttttta tctaaatcaa
     2761 ctaactgttt ttcttttaaa atatgtctac catctatgta tacatttgat ataaattctt
     2821 ttttaccctg aactactatc tggtcaaata tattatcaag agtcacagga gttttaaatt
     2881 catcttccat aactattata tcagccttat taccaactgc tattttacca gaattttgaa
     2941 gtcctaatac ataagcacca tgctctgttg ccattctaaa tactaaattt gctggcatta
     3001 ctgatgcatc ttcttcaact gatttatgca ttaaaaatgc acctctcata acttcaaaaa
     3061 aatcatttat atatccatca gtacccagtg ccacttttat gcctttttta atcatttttg
     3121 gtactggaga aaaacctcca cctacttcac agttactaag aggcatatga accactttta
     3181 cgcctttttc ttttaatata tctatttctt catcatttac ttttacacac tgagatgcta
     3241 atacagtttc atccaatata tctaaatcat tatagtagtc aactgctttt ttgccaaagt
     3301 gtttttcagc atagtttggc tcatatatac tctcacataa atgaaattgc attggtgcat
     3361 ttaatttttt tgcatcttct ttagctttct taataaatct atcagaacat gtgaagcttg
     3421 tatgtgtaca cattattcca tttattaatt tactattttc tctactccac tttattaaat
     3481 tagaattttc atctaaacat ctaagtccat tttcaaagct tattctttca caactttcta
     3541 aggataaaat cgctttcatg cctatttctt ctagtatttt tccttgctca attaaagttc
     3601 cttcttctgt atttggagct tctagggtat cacaaaaagc taccacacca gattctataa
     3661 gttctattgc tgatgcttta gttgttgcct tcacttcttt taatccaatt ctattttcta
     3721 taaatggcca ccaatagtca tttaaaaaac cttcaaagtc agtaaaatgt acatttgccg
     3781 gtattcccct agaaagaact ccatattgat gcatatgacc attgacgaat cctggcatta
     3841 ttatagcatc tgatttatct attatttctt caatattttt atattttatt tcaacttctt
     3901 catttggcaa tatatctttt ataatgtcat tttctactac aacagcagaa tttgtatata
     3961 tttcatcagc agaagatatt aagtatttac ttttaatagc aatcatttat ttacctccct
     4021 gatttaattt tttcatggcc atataaactt tctcagaagt tataggaagt tctcttatac
     4081 gaactcctgt ggcattgtat attgcatttg ctattgctgg tgcaggtgta tgaataacta
     4141 tttctcctaa agatttagct ccatatggac ctgttggctc ataattatct acaaaatcaa
     4201 caattatgtt tcctatatct ttttttgttg gaactttgta ttgtaataga ttagaagttt
     4261 gaagctttcc atcagaacca tgtctagggt cttcatacat tgcaagccct ataccttgag
     4321 ttacaccacc ttcaacttgt attctagcaa gtgcaggatt cataactgta ccacaatctg
     4381 tagaacaagc ataatttatt actttaaact cccctgttgt tttatctaac tctatttcag
     4441 caaatcctgc taagaatggt ttagcactct cttgtatacc ataagattca gatgaatata
     4501 aaacttcttg atttccacca ccaacagatt cttttcctaa ttcacttaac aaaacaaact
     4561 tctctacatc ttcactataa caaactctgt cttcatatag tattaaatca tctattggta
     4621 aatcgagttt cttctgtgct acttttaata tcttttgttt taaactttca gcagatttta
     4681 ttgtagcatt tccagttaca taagtagtac aagaagcgta tgcaccagta tcatatggac
     4741 acatatctgt atctcctgta taaacggaaa ttctatcaat agatgtattc aatgcttttg
     4801 cagctatttg tgcaagtatt gtgtcagaac cagttcctaa gtctgaagac ccagaaaaca
     4861 acttataagt tccatcttca tccatcctca tatttactat tgccatgtct atgttggcta
     4921 taccagaacc atgagttccc acagacattc caacagccct caccttattg tttccaatat
     4981 ccctaacagg gtacttttca tcccatccaa taagtttttt acctctaatt atacattcat
     5041 caagtgcaca acttcttatt ggaaaattca ttattccgcc ttcagtttgt ttttttataa
     5101 tattctttaa ctttatttct gttgggtcca tattaagttt atgcgctaat tcatcaattg
     5161 cacaatcaag tgcaaatgta ccttgtgttg ctccatatcc tcttaatgct cctcctggaa
     5221 ctaaattagt atatacagtt ttcccatcaa attttattgc aggtacatta ttatatgttg
     5281 gaagtacatt atgacctgat tcagagcaaa ctgaaggtcc attatcgcca taagcgcctg
     5341 tattgttcaa tgctttcata tctatagctt ttatgtttcc ttctttatct gccccaattt
     5401 ttatatcaaa aaacatctga tgtctagtat tagtcattgc aaaagtttct tctctagtat
     5461 aaattagttt tgctggtctt tttgtcatcc aagttacaaa tgatgcatat atctctgtta
     5521 cagctatgtt ttttccacca aaacctccac ctattcttgg ttttattact ctcacttttg
     5581 aaagaggtat tcccagtgct ttagaaacct gtcttctcat atgataagct gattggtttg
     5641 cagaagttac tactaatctg ccattcgtat ctatatacgt ataagctcta tgagtttcca
     5701 tcatacaatg ggcttgtgat tgagtcatat aagaatgttc tactattaca tcactttctt
     5761 taaactcttt ttctacatcc cctttaccca ttttttctct cgatacaata ttttttgtag
     5821 catccaatcc aaaatcaaat ggacaaaaca aatcatcttc ttcatggatt aaaattttat
     5881 ttccttccga ttctcttgca tccaatatag gagtcattac ttcatattca acttttataa
     5941 gtttcatagc tttaatagca gccctttcat cttcagcggc tattatagca acttcatctc
     6001 ctacatatct aactacgtct tcaagtatta attggtcatg aggtgatgct tctggatatg
     6061 actctccaac cattgaatat ctataatttg gaacatcttt gtaagtgaat atgtttacta
     6121 caccttttac ctttaatgca ttttttatat ctatgttttt tatcttcgca aaagcatgtg
     6181 gacttctaag aagcttaatt gttaaaacat ctttatgaaa aattaaatct tctgtatata
     6241 ttggttttcc agtaacaatg gcttcactat ctatttttct tacctcttta tttaccatca
     6301 cactcacctc caatactatt atacattctc actactaagg cttttgtcat atcttttctg
     6361 tattctttac ttcctcttat attacttcca aatgaaagtt cttcacttga tacttcacca
     6421 gctttttcta tatcattttc taaacttaat atttcacttg ctttataggc aatttttgct
     6481 cttcttggtc tagcgcctat cgcaatttta taaatacttc cttcttttaa cattgctcca
     6541 tttaatactg caaaatcacc tgtacatttt cttatagaat caaatacacc tatagcatct
     6601 tttttaggta atataacttc tattaatata tctttttcaa gctcattttc taaaaaatca
     6661 gctaagttca taaccccttt tttatataac ttaacctttg catctaccac tagtaatgaa
     6721 ggtattaaat ctgaaaatcc atacttagca aatacgctag ctcctactct agctccactt
     6781 ctaaattgaa ctcctactat attagaaaca gcgtttgaaa ttaccccatt acagtaattt
     6841 ttaataacct tatttaattc taaagttctt aaacttgtat ctgctccaat taatatgcta
     6901 tcatcagttt catttatata atctaaagct aagtctttta aatctattgc tgaaccaata
     6961 tttttactac ctagttttaa aaatccacat cctccaagta ttatgttgtt ttttcttgat
     7021 attagcagtt cataagcttc atctaaactt tttggaacta catattcctt tatagttatc
     7081 atactctctc cccatatcaa cttaattttc acttaaaagt ctaagtgttt aaatatttat
     7141 gtattgctct taattgacct ttatatcctg tacatctaca aaggttacca tttacataat
     7201 ctataacttc ttcttctgtt ggattttgaa gctctctttt cattgccaag accatcatta
     7261 taaatccagg agcacaaaat ccacactggt cagccccttc gtctgcaaga aaatctccaa
     7321 acttctttgc ttcatccaag acaccctcaa gtgttgtaat atgctgacct tctactctag
     7381 cagtaagata attacatgaa agcacggcct tgtcatttac catcactgta cataacccac
     7441 atgagcctgt atcacaccct tttttaacac ttacatatcc attttctctt aatgtatctg
     7501 ctaaaaaatc atctggtctt atacttaatt ctttttcttt attatttatg gtacatttga
     7561 taatcaaata aatcacctca aaactcatat ttttaagcta aagttttact atactttttt
     7621 atgaataaat tacatttatt aataaaggct attccattta tgaccttcca ttctattttt
     7681 tatagttcct cttatacaaa gtctctcagc tgtatcttga gcatctttta atgctttctc
     7741 ttcattagct gtcaatattt ttgaatcttc cattattata tttccatcta ctataacact
     7801 ctctatattt ttcatactag aagaatatac taatgtagat accggattat gcataggtat
     7861 cgcttttggt gaaagcattg gattaaatat tagtaagtct gcttttttac ctatctccaa
     7921 tgaacctatc tcatcttcca ttccaatagc tcttgctcca tctatagttg ccaactcaag
     7981 aactttttct gccgacattg ctaatgggtc acacttatta actttatgtt gaagtgctgt
     8041 taatttcata agttcaagca tatcttgaga gttattacta gcggctccat ctactcctaa
     8101 actaactgtt attccttttt taagcatttc tggcactggt gcaacacctg aagataagta
     8161 catattactt gctgtattat gagacacttt catatcgtat tttttagtaa gttccatatc
     8221 tttttcagtt agatacacac aatgtaccat aagcacattt ggtccaagta ttcctaattt
     8281 ttctaaaaca tctatgtcat attgcccatg caattctttt gctgcttctc tatcaaaagg
     8341 agtctctgat atatgtactg taaataaagc atcatcatat tcttttacta ctctccatag
     8401 catttcaagc atttcttgag aatttgacca tattgctgca ggagctactc ctattttgat
     8461 tcttccattt tctgtattat ggtgtttttc aaacaatctt cttacatctt tttcaaccgt
     8521 ttctacatct tgcattattc caggatgaac tccaaattga gctcctgtat tcatacatcc
     8581 tcttccaagt attcctctta ttcctaattc tttataagca tctattattc catcacaaag
     8641 acctggctta ctatgaggat acatgtagtc aaccattgtt gttataccac ttctaagtcc
     8701 ttcaatacat ccaagcattg cagcatcata agtatctttg ggctctaaat agttagctgc
     8761 tggaaatgtc atagtttcta accagtcttt aagtaccata tcatctccca atccttttaa
     8821 taaagtttgg aataaatgat tatgtgtatt tataaaacct gggaataaaa ctttaccttt
     8881 agcatctata atttttttaa catcagtata tttactttct atctctttag aatttccaat
     8941 atcagcaatc ttgttatctt ttactactaa tgccccatca aaaataactt ctctctcttt
     9001 attcacagtt actattatgg cattcttaat aagtatatcc atcattctta tttccccccg
     9061 cattatttag taatttttct tttaatatat tgacctgttg gatgttcttt aacattacaa
     9121 ctttgtacta catgatttcc tctcaaaaat acatcttcaa ctttacattt aaccttaaat
     9181 ccttcatatg gagtataatc tacattttgt ttttgagttt tataactaat tgtttcttct
     9241 ttattgatat ttaaaatgac tatatctgca tcacttccta cagctatctc acctttttta
     9301 ggatacattc caaatatttt tgcagcattc gtagaagtaa cttccacaaa cttatttaca
     9361 cttattctat tttctaagac cccatatgta tataaaagtg ctaacctatg ttcaacacca
     9421 ggtgctccat ttggtatctt actgaaatca tcaattccca agtctttttg ccccttaaaa
     9481 ttaaatgaac agtggtctgt gccaactgtt tgaatatctc cactagacaa tccttcccaa
     9541 aggtaattta tgtcttcttt ttttcttaaa ggaggagaca ttacatactt agcaccctca
     9601 aatttatcct tattatataa attgtcttct aataatagat actgtgggca tgtttctact
     9661 atcatatcta tatttttttc tctagctatt tttacagttt caagtgactc cctacaactt
     9721 aagtggacta cataggattt tgaattagat aatttagtta tatcagcaag tcttgaaaca
     9781 gactctttct caactatgtt aggtcttgta agtggatgat attttggctc caaattacca
     9841 ttttgtatat tttcttctat caatacatct agtaaatccc cattttcaca atgaaatcca
     9901 actatacaac ctaaatccct tgctttcttt aaaaccctgt atattgttcc atcatctact
     9961 ttcatgccat catatgccat atacatctta aaagatacta tcccatctga aataagtttt
    10021 tccatatgat taaatgtatc tttatttaaa catgtaattg tcatatgaaa accataatca
    10081 caataacaat ttccacttgc ttttttatga tatgcttcta caccttgcag tagattttcc
    10141 ccctcattgg cttctgcaaa atctagtatt gtagtagttc ctccagctat agctgctctt
    10201 gttcctgttt caaaattatc tgcagttgta atagaacctg cattcatatc aaaatgagta
    10261 tgtgtgtcta ttccaccagg aattacaatt ttacctgtag catcaataac cttatcacca
    10321 tttatttcta agttatttcc tatttcaact atcttttcat cttctatcct taaatcactt
    10381 atataagtct tatcagaggt aattatagtt ccacctctta gtattgttcc catatatacc
    10441 tcctaacttt taagctttaa agtattttct aagtattgta aaactttatt taaaattgta
    10501 ttttctaaag gtgctatatt cagcacctct agactatatt taaataattt attgtgaact
    10561 aaatattatt ttccgtatac agattttaat attccatagt aaccttctgt tcctataaat
    10621 aactgttctt gctctatata ctcatctatt gtgtgagcaa gattttcctt agaaggacca
    10681 aatcctatag tttttattcc agcttcacct gcatagtgac ttccattcgt acagaatgag
    10741 tattgagtta tttctgaatc tattccagct tcttttaatc ccttatacgc tgcttgaaca
    10801 aattcatctt cttcgtcata taaccaacct gggaagaatc tttcactttc tatagtattt
    10861 cctgtatagc aatcagcttt ttcaac
//
LOCUS       DAMJVK010000117        10742 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.122, whole genome shotgun sequence.
ACCESSION   DAMJVK010000117 DAMJVK010000000
VERSION     DAMJVK010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10742
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.122"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            153..1337
                     /locus_tag="PC414_003252"
     CDS             153..1337
                     /locus_tag="PC414_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465625.1"
                     /translation="MQKSVKKAKVTGSMLAIFGVASVLFSSHAGGGFATGNQETQYYV
                     QYGWTAPLMAILAMIILTATMREVIIMYNNNNCRNYKDLFCELWRPYPKLEIIWEIYY
                     YLMVLIAVSAVIAGAAAVFQSIGVNYFVAVFIIGVVLLVFTIFGAMLVSKAATAMTIA
                     ILVCTLTIFIVGIKAKVPEITEILSNRTSFTPGYIKPILNTFIYAGFQSVVIPTLAGC
                     SRPLLKNSKEATKAMIISFVMNAIALGLAVTMLMGWYREIIAAGQTTLPTLYVAGQSG
                     NHTIYIIYNVALFLCLMSTGVTTIFGLVNRFEDHKALSFLSSRMKRRVFTACAIMVVS
                     MLISLTGLSNIVKYGYGYCGYLGLFTIVIPFLTLGHYKNKKFAKENPEAKWPAELNEN
                     VN"
     gene            complement(1714..2823)
                     /locus_tag="PC414_003253"
     CDS             complement(1714..2823)
                     /locus_tag="PC414_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF2465626.1"
                     /translation="MDFKSLGISENTINILKKSGITTPTPIQKESIKLIKEGKDVIAE
                     AQTGTGKTLAFLLPIFENISLDINDIQVLILSPTRELAIQITEEAMKLKESKDVSILA
                     AYGGKDIGSQIKKLKGNIHMIIATPGRLLDHLNRKTIDLSKLKTFVLDEADQMLLMGF
                     KNEVEAILKETSNKKQTLCFSATINSQVKKLAYRYTKNPVVVSIQKEEITLNNIKQEV
                     VETTDRKKLDALCKVLDEDNPFMAIIFCRTKRRVDNLEEALAIRGYNCQKLHSDIAQS
                     KRERIMKSFRNLDIQYLIATDVASRGLDISGVSHIYNYDLPETPEDYIHRIGRTGRAG
                     EEGYTCAFIDPKNERMLSEIETAIESKISRRIIEL"
     gene            3286..4479
                     /gene="nifV"
                     /locus_tag="PC414_003254"
     CDS             3286..4479
                     /gene="nifV"
                     /locus_tag="PC414_003254"
                     /EC_number="2.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HDF2465627.1"
                     /translation="MCVISKDRAKEIKIVDTTLRDGEQTAGVVFANREKIMIAEMLSD
                     LGVDQIEVGIPTMGGDEKNVIKHICSRNLKSDIMAWNRAVIKDVEESISCGVDAVAIS
                     ISVSDIHIENKLRTSRGWVLENMAKTVEFAKKNGLYVSVNGEDASRADIDFLTEFINV
                     GKQAGADRFRYCDTVGVMNPFSIKNAIETLYERTNFDIEMHTHNDFGMATANALAGIA
                     AGANYVGVTVNGLGERAGNAALEEVLMALKCVYKCDLNNIDTRKFRGICEYVAQASGR
                     ILPTWKPVVGDNMFIHESGIHADGALKDPHNYEPFDPSEVNLERKIVIGKHSGRAAVV
                     NKLSEYEMYISPENATKLLNAIRATSIRLKRSLMDKEILQLYCDILAHEKGTTEEEAV
                     RGSYI"
     gene            4504..6429
                     /locus_tag="PC414_003255"
     CDS             4504..6429
                     /locus_tag="PC414_003255"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HDF2465628.1"
                     /translation="MGDNIVYKIIKKHIVDGEAVAGSSIGIKIDQTLTQDSTGTMTYL
                     QLEAMGIDKVKTKRSVAFVDHNMLQQGFENADDHKYIQTVADKYGVYFSKPGNGICHQ
                     VFLERFSTPGDTLLGSDSHTPTAGGVGMMAIGAGGLDVALAMAGGAYYIKAPKVCKVN
                     LVGKLNNMVSSKDIILEVLRKQTVKGGVGKVYEYGGEGVKSLSVPQRATITNMGAELG
                     ATTSIFPSDEKTLEFFKSQGREDAWVELKPDADAVYDEEITINLDELKPLAAKPHSPD
                     NVDEVENIGKIKIDQVAIGSCTNSSYEDLMKVAQILKGNKVHKDVSLVIAPGSRQVME
                     MIARNGALADIISAGARILENSCGPCIGMGQSPGTDSVSLRTFNRNFYGRSGTLSAQV
                     YLVSPEVAAVSAIKGVLTDPREFDIKFTNLDVNEFLIDDSMIIKPADVGSDVEVVRGP
                     NIKPFPLNTELSQSIGGKVILKTEDNITTDHIMPSNAKLLPFRSNIPYLANYCFNTVD
                     TEFPQRAKDNNGGFIVGGDNYGQGSSREHAALAPLYLGVKGVIVKSFARIHKANLINS
                     GIIPMEFCDEKDYENISLLDNLEIPNILDNLGSGILEVKNTTKGTSFKVKVELSAKEV
                     DVLKAGGKLNYTKNQAN"
     gene            6451..7446
                     /locus_tag="PC414_003256"
     CDS             6451..7446
                     /locus_tag="PC414_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HDF2465629.1"
                     /translation="MYKVTLIPGDGIGPEVAKAMKKVVEATGVEIEWEEVNAGEAVIE
                     EYGTPLPEYIIDSIKKNKIAIKGPITTPVGKGFRSVNVTLRQALDLYVNLRPIKSFKG
                     IKSRYEDVDLVVVRENTEDLYAGIEHKIGDYAAESIKIITRSASERIVDFACNYVKDN
                     KRKKVTAIHKANIMKMSDGLFLDVFREVASKHGVEYDDLIVDAAAMNLVLNPENYDVM
                     VMPNLYGDILSDLGAGLVGGLGIIPSANIGKDCAIFEAVHGSAPQIAGQNKANPTALI
                     QSSVMMLRYLGEYENAQKIETALEKVFLEGSKLTVDLGGSASTTEFADEVCKYIV"
     gene            7771..8244
                     /locus_tag="PC414_003257"
     CDS             7771..8244
                     /locus_tag="PC414_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2465630.1"
                     /translation="MVVSVEVCLIKTLDSNILREVGTLSRAVNSINDIKYKELKLQKG
                     QFTFLTRICENPGINLVELSNMLKVDKATTTKAIQKLIKAGYVDKKQDKFDKRGYNLT
                     PTDKSLEVYELIIEEENRSIEICFDNFTDEEKQVVTKLLEKMSKNVENEWFKVKR"
     gene            8278..8772
                     /locus_tag="PC414_003258"
     CDS             8278..8772
                     /locus_tag="PC414_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2465631.1"
                     /translation="MIRKANMNDLESIMKIIKSTVEEMKTYNNTQWDENYPLEKDFVS
                     DIKKQDLYIYEVDGEVAGFICLNYEEPEEYLGLNWSSNKKAMVIHRMAVNPNFRKTGI
                     ASKLVDFAEKLAVENNVSYLKSDTYSINSKMNLLLTKCGFIKIGEMSFLGKEKSFYCY
                     DKIL"
     gene            8869..9390
                     /locus_tag="PC414_003259"
     CDS             8869..9390
                     /locus_tag="PC414_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2465632.1"
                     /translation="MIGFLKKRRSIRKYKDVEVEKEKLDKILKAALLAPSSKGLRTWE
                     FIVVDDKEKLINLSQCRTKGGGFFLKNAPLAIVIIADKEKNDVWIEDASIAASYIQLQ
                     AHELGLGSCWIQVRNRMHDDNIEADKYIREELKVPSKYSVECIISIGYPDEEKKAYGD
                     SDLDYKKVHFNNF"
     gene            9579..9890
                     /locus_tag="PC414_003260"
     CDS             9579..9890
                     /locus_tag="PC414_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDF2465633.1"
                     /translation="MGIFKTKMDEDWKVNYIKEFNEMRDSYESKLQKKQFEVDSLKSE
                     LDRLRSYKNSLKPKEKQITDDDINNIKSLRRDGLSYKEISNQTSWSKATVSRVLNGLY
                     D"
     gene            9918..10604
                     /locus_tag="PC414_003261"
     CDS             9918..10604
                     /locus_tag="PC414_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF2465634.1"
                     /translation="MKYSYKKCIIDSILLMFIVQILRMILNYVLLSQFEFTLENFNII
                     NLISFTLVGLSLILFLKDNSLYNKVRNRKITEAFEENKNNILIEKCKLILFVVVLSLA
                     IIVTYCTKGYVLFNVTMMTLSVLIVPIFEELFFREYIWNYLSNFIKSKSKIICITSIL
                     SGIYNIGYIDVIRNYVILYNNSSYTFEVIISKIMIGTVFGIVLGLVKYRFRDVGFCIL
                     LRSLFAIFIR"
BASE COUNT         4101 a         1169 c         1942 g         3530 t
ORIGIN      
        1 tataaaaaat atttaatata aaggtggaaa taagttgaaa atgtaaagtc attggaaaaa
       61 tatagttgaa tttaatgagc aaaaatgtta ttgttttatc aataaagtgt taaaataaaa
      121 actttcaata tcattttagg gaggatgatt tcatgcaaaa aagtgtaaag aaagcaaaag
      181 taacgggctc tatgttagct atttttggtg ttgcaagcgt tctttttagt tcacatgcag
      241 gaggaggatt tgctacaggg aatcaagaaa ctcagtacta tgtgcagtat ggatggacag
      301 caccacttat ggcaatttta gcaatgatta tacttactgc taccatgaga gaagttatca
      361 taatgtataa taataacaat tgtaggaatt ataaggattt attttgtgag ctttggagac
      421 catatccaaa acttgaaata atatgggaga tatattacta tctaatggtt ttaatagctg
      481 taagtgctgt tatagcaggt gcagcagcag tatttcaaag tataggagta aattattttg
      541 tagctgtatt tattattgga gttgtacttc ttgtctttac tatatttggt gctatgcttg
      601 tatctaaagc agctacagca atgacaatag ctattctagt atgtactctt actattttta
      661 ttgttggtat aaaagctaaa gtaccagaaa taactgaaat attatcaaat agaacgagtt
      721 tcacacctgg ctatatcaag ccaatactta atacttttat ttatgcaggt tttcaatcgg
      781 ttgttatacc tacacttgca ggatgttcaa gaccacttct taagaactca aaagaagcta
      841 ctaaggcaat gataatttca tttgttatga atgcaattgc attaggactt gctgtaacta
      901 tgctaatggg atggtataga gaaattattg ctgctgggca aacaacactc cctactcttt
      961 atgttgcagg tcaatctgga aatcatacta tatacataat ttataatgtt gcattattcc
     1021 tttgtttaat gtctacagga gtaactacta tatttggtct tgtaaataga tttgaagacc
     1081 ataaagcact ttctttcctt tcttcaagaa tgaaaagaag ggtatttaca gcttgtgcaa
     1141 taatggtagt gtcaatgctt atatcactta caggattaag taatattgtt aaatatggat
     1201 atggatattg tggttatctt ggtctattca ctatagtaat tccatttctt acactaggac
     1261 attataagaa taagaagttt gcaaaggaaa atccagaagc taaatggcca gcggaattaa
     1321 atgaaaatgt aaattagatg tagtagcaaa aaatattgct aaagagaata ctataaataa
     1381 gaatactata gataatatat ctgcatgttt attaagatta tttgcaaaat ctaatattaa
     1441 acaactataa acaagcaata taaaactaag aataaaagat aaaaagaggg tatctcaaaa
     1501 tggactttta gtttataagg cactcttttt tatatgaagt caaatatatt ttcattattt
     1561 tgacaataaa agagactaat cctactttgg atagcctctt ttttctttaa tataaaatag
     1621 tataacagca aagtatagat tgatacatgt tttctgtagc tttctatata ttacagtgtt
     1681 tgtgtggcat tggtatttta agcaatctat aaattataat tctataattc ttctactaat
     1741 tttagattct atagccgtct ctatttcact taacattcgt tcatttttag ggtcaataaa
     1801 agcacatgta tagccttctt caccagctct accagttcta ccaatacggt ggatgtagtc
     1861 ttctggtgtt tctggtaaat cgtaattata tatatgactt actccagata tatcaagacc
     1921 acgagaagct acatctgtag ctattaagta ttgtatatct aaatttctaa atgatttcat
     1981 tattctttct cgttttgatt gagcaatatc actatgaagt ttttgacaat tatatcctct
     2041 tatagcaaga gcttcttcaa gattgtcaac ccttctctta gttctacaaa atattattgc
     2101 cataaaagga ttatcttcat ctagtacctt acataaggca tctaattttt ttctatctgt
     2161 agtttctact acttcttgtt ttatattatt aagagtgatt tcttcttttt gaatagaaac
     2221 tacaacagga ttttttgtgt atctataagc taattttttt acttgagaat ttatagttgc
     2281 agaaaagcat agtgtttgct ttttgtttga agtctctttt aaaatagctt ccacttcatt
     2341 tttaaatccc ataagcaaca tttggtctgc ttcatctaaa acaaaagtct ttaacttact
     2401 aaggtctatt gtttttctat ttagatggtc taaaagccta cctggtgtag ctataatcat
     2461 atgaatattt ccttttagtt tctttatttg agaaccaata tcttttcctc catatgctgc
     2521 aagaatactt acatctttag attctttaag tttcatagct tcttctgtta tttgaatagc
     2581 tagttctctt gtaggagaaa gtataagtac ttgtatatca tttatatcta atgatatatt
     2641 ttcaaatatc ggaagtaaaa aagctagtgt ttttcctgtt ccagtttgag cttctgctat
     2701 tacatctttt ccttctttta ttagtttaat actttctttt tgtataggtg taggtgttgt
     2761 tattcctgat ttttttagaa tatttattgt attttcacta atccctaagc ttttgaaatc
     2821 cattgttatc ttacctttca tgaaaaattt tcttttttat taaccaatta ataatatcat
     2881 atttgtaagt atttttagta aattttttaa ttattattta tatagcttaa aaatatatta
     2941 agatacttta aaatggaatg taacttaaat taaattttag taggaatttt ataggtttat
     3001 ttttaaaggt tttttgaaaa taaatctatt taaaaattaa attaaaatgc aagaaaaaca
     3061 taataacttt catttttaag aagttttcac aaaaaagaaa aaatatagtg caataaatga
     3121 tacaaagtaa tataatagat tacaagatat gaaaagctaa aaaaataaat tgcaaatgaa
     3181 attaaaatga ataaaaaata aaaaaataat cataagttag aagtatcaat gaaaaataag
     3241 acttaatgtt tttaaaatta aaagtatact aaggggggca ttaatatgtg cgttatatca
     3301 aaagatagag caaaggaaat aaaaatagta gatactactt taagagatgg agagcaaaca
     3361 gctggggttg tatttgcaaa tagagaaaaa ataatgattg ctgaaatgtt aagtgaccta
     3421 ggtgttgacc aaattgaagt tggtatacct actatgggtg gagatgaaaa aaatgtaata
     3481 aagcacatat gtagtagaaa tttaaaatca gatataatgg cttggaatag agcagtaata
     3541 aaagatgttg aagaatctat ttcttgtggt gttgatgcag tagctatatc aatatcagta
     3601 tctgacattc atatagaaaa taaattgaga acttctagag gctgggtttt agaaaatatg
     3661 gctaaaacag ttgaatttgc aaagaaaaat ggattatatg tttcagtaaa tggagaagat
     3721 gcttcaagag cagatataga cttcttaact gaatttataa atgttggaaa acaagcaggg
     3781 gcagatagat ttagatactg tgatacagta ggagttatga atcctttttc tataaagaat
     3841 gcaatagaaa ctctttatga aagaactaat tttgatatag aaatgcatac acataatgac
     3901 tttgggatgg caactgcaaa tgcacttgca ggaatagcag caggagcaaa ttatgttggt
     3961 gttacagtaa atggattagg tgaaagagca ggaaatgcag cattagaaga agttttaatg
     4021 gcacttaagt gtgtttataa atgtgattta aataatatag atactagaaa atttagagga
     4081 atatgtgagt atgtagcaca ggcatcagga agaatacttc ctacatggaa accagtagtt
     4141 ggggataata tgtttataca tgaatctggt atacatgcag atggagcttt aaaagaccca
     4201 cataactatg aaccatttga cccaagtgaa gtaaatcttg aaagaaaaat agtaataggt
     4261 aaacattcag gaagagctgc agttgtaaat aagttgagtg aatatgaaat gtatatttct
     4321 ccagaaaacg ctaccaagct attaaatgct ataagagcta catctataag attaaaaaga
     4381 agtttaatgg ataaagaaat tcttcaatta tattgtgata tactagcaca tgagaaaggc
     4441 acaacagaag aagaagcagt tagaggttca tatatttaag atataattgt ttaggaggaa
     4501 aatatgggag ataatatagt ttataaaata attaaaaaac atatagtaga tggagaagct
     4561 gttgctggaa gttcaattgg tataaaaata gaccaaactt taacacaaga ttctactgga
     4621 acaatgacat atcttcaact agaagctatg ggaatagata aagtaaagac taaaagaagt
     4681 gtggcatttg tagaccataa tatgttacaa caaggatttg aaaatgcaga cgaccataag
     4741 tatatacaaa cagtggcaga taaatatgga gtttatttct ctaagccagg aaatggtatt
     4801 tgtcatcaag tatttttaga aagattttca acaccaggag atacactttt aggttctgat
     4861 agccacacac ctactgctgg aggtgtgggc atgatggcta ttggagctgg tggattagat
     4921 gttgctttag ccatggcagg aggagcatac tatataaaag caccaaaagt ctgtaaggtg
     4981 aatctagttg gaaaattaaa taatatggta tcatcaaaag atataatatt agaagtgtta
     5041 agaaaacaaa ctgtaaaagg tggagtaggt aaagtttatg aatatggtgg agaaggtgtc
     5101 aaaagcctat ctgttccaca aagagctaca ataacaaata tgggggctga acttggagca
     5161 actacttcaa tcttcccaag tgatgaaaaa acactagaat tttttaagag tcaaggaaga
     5221 gaagacgcat gggtagagct aaaaccagat gcagatgcag tttatgatga agagattact
     5281 ataaacttgg atgaattaaa accattagct gcaaaacctc atagcccaga caatgtagat
     5341 gaagttgaaa atataggcaa gattaaaata gaccaagtgg caataggaag ttgtacaaat
     5401 tcttcttatg aagacttaat gaaagtagca caaatcctga aaggaaataa agtacataag
     5461 gatgtgagtt tggtaatagc accaggttca agacaagtta tggagatgat tgctagaaat
     5521 ggggctttgg cagatataat aagtgctgga gctagaatac tagaaaattc ttgtggtcca
     5581 tgtattggta tgggtcaatc tccaggtact gactctgtgt ctttaagaac tttcaataga
     5641 aatttctatg gtagaagtgg gactttgtca gcacaagtat atttagtaag tccagaagtg
     5701 gcagcagtaa gtgcaatcaa gggagtgctt acagacccaa gagaatttga tattaaattt
     5761 acaaatttag atgtaaatga gtttttgata gatgatagca tgataattaa acctgctgat
     5821 gtaggaagtg atgtagaagt agttagagga cctaatataa aacctttccc tcttaataca
     5881 gagctaagtc agtctatagg tggaaaagtt atattaaaga cagaagataa tataactact
     5941 gaccatataa tgccatcaaa tgctaagtta cttcctttta gaagtaatat accgtatctt
     6001 gcaaactatt gtttcaatac tgtagataca gaatttcctc agagagcaaa agacaataat
     6061 ggtggattta tagttggtgg agataattat ggtcaaggtt caagtagaga acatgcagct
     6121 ttagcaccat tatatctagg tgttaaaggg gtaatagtta agagctttgc aagaatccac
     6181 aaggctaact tgataaatag tggtataatt ccaatggagt tttgtgatga gaaagattat
     6241 gaaaatatat ctcttttaga taatctggag ataccaaata ttttagacaa tctaggtagt
     6301 ggaattttag aagttaagaa tacaactaaa ggaacttctt ttaaggtcaa agtagaattg
     6361 tcagcaaaag aagttgatgt attaaaagct ggtggaaaat taaactacac taaaaatcaa
     6421 gctaattaat atgaaaagag ggaatattga atgtataaag tgacacttat accaggagat
     6481 ggaataggac cagaagttgc gaaagctatg aaaaaggtag tagaagctac tggagtagaa
     6541 atagaatggg aagaagttaa tgcaggagag gcagttattg aagagtatgg tactccactt
     6601 cctgaatata ttatagatag tataaaaaag aacaaaatag ctataaaagg cccaattact
     6661 actcctgtag gtaaaggatt taggagtgta aatgtaactt taagacaggc attagatttg
     6721 tatgtcaact taagacctat taaaagtttt aaggggataa aatcaagata tgaagatgtt
     6781 gatttagtgg ttgtaagaga aaatacagaa gatttatatg ctggaataga acataaaata
     6841 ggtgattatg cagctgaaag tataaagata ataacaagaa gtgcaagtga aagaatcgtt
     6901 gattttgcat gtaactatgt taaagataat aagagaaaaa aagttacagc tatacataag
     6961 gcaaatataa tgaaaatgtc tgatggattg tttttagatg tgtttagaga agttgcatca
     7021 aaacatggtg tagaatatga tgatttgatt gtagatgcag cagctatgaa cttagtttta
     7081 aatccagaaa attatgatgt aatggtaatg ccaaatcttt atggagatat actatcagat
     7141 ttaggtgctg ggcttgttgg aggtttaggt ataattccaa gtgcaaatat tggtaaagat
     7201 tgtgcgatat ttgaagctgt tcatggaagt gctccacaaa tagcaggtca aaataaggct
     7261 aatccaacag cacttattca atcatcagta atgatgctta gatacttagg tgaatatgaa
     7321 aatgcacaaa aaatagaaac agctttagaa aaagtatttc ttgaaggaag taaattgact
     7381 gtagatttag gtggctcagc ttctactaca gagtttgcgg atgaggtgtg taagtatatt
     7441 gtatagattt tatttaggga aaaagtaaaa tatagattga agaaaagagg ctttctcaaa
     7501 atagagataa gcctttttta ttggaatgat aaaatatatt tgattttata caaaagagag
     7561 ttcctcataa acgaaaaagt taattttggg atatccattt taattttaaa attaattgat
     7621 taatacgcaa agaaagatta aataaattta ttatttttaa ttgaactttc aaccaaaata
     7681 tggtaaaatt taaaaagttg aactttcaac taaaaatgaa aatattgatt atgtgaaatt
     7741 ctataaaaca gaaactattt gactttagcc atggtagttt cagtggaggt gtgtttgatt
     7801 aagactttag atagtaatat attaagagaa gtaggtactt tatctagagc agtaaattca
     7861 ataaatgata ttaagtacaa ggaattaaag ttacaaaaag gacaatttac atttttgaca
     7921 agaatctgtg aaaatcctgg tataaatctt gtggaattat caaatatgtt aaaggtagac
     7981 aaggctacaa caaccaaggc tatacaaaaa ttaataaagg caggatatgt agataaaaag
     8041 caggataagt ttgataaaag aggatataat ttaactccaa ctgataaatc tttggaagtt
     8101 tatgagttga taattgaaga agagaataga agtatagaga tatgttttga taattttaca
     8161 gatgaagaaa aacaagtggt aactaagtta ttagaaaaaa tgagcaaaaa cgtagaaaat
     8221 gaatggttta aagtaaagag atagtaaata aatacattaa cttaaggaga tatttttatg
     8281 attagaaaag cgaatatgaa tgatttagaa agcattatga aaattataaa atctacagtg
     8341 gaagaaatga agacttataa taatacacaa tgggatgaaa actatccatt agaaaaagac
     8401 tttgtttcag atattaagaa acaagattta tatatttatg aagtggatgg agaagtagca
     8461 gggtttatat gtcttaatta tgaagagcca gaagaatatt taggcttaaa ttggtcatct
     8521 aataaaaaag caatggtaat acatagaatg gctgtaaatc caaactttag aaagacagga
     8581 atagcatcta agttagttga ttttgctgaa aaattagctg tagaaaacaa tgtatcatac
     8641 ttaaagagcg atacatattc aataaactca aaaatgaatt tattacttac aaagtgtggt
     8701 tttataaaaa tcggagaaat gagtttttta ggaaaggaga aatctttcta ctgttatgat
     8761 aaaatattat aaagagcatt tataatacaa ggggtaaata atagattttc agcattagta
     8821 agggcataat ataacctagt atatgctatt atgaaaggga tgatttatat gattggattt
     8881 ctaaagaaaa gaagaagtat tagaaaatac aaggatgttg aagtagaaaa agaaaaatta
     8941 gataaaatat taaaagctgc tcttttagct ccatcatcaa aggggcttag aacatgggaa
     9001 tttatagtgg tagatgataa agaaaagcta attaatttat ctcaatgtag aactaaaggt
     9061 ggtggatttt tcttaaaaaa tgcaccttta gctattgtaa ttattgcaga taaagaaaaa
     9121 aatgatgttt ggatagagga tgcaagtata gctgcttcat acatacaact tcaagctcat
     9181 gaactaggtt taggttcttg ttggatacaa gtaagaaata gaatgcatga tgataatata
     9241 gaagctgata agtacattag agaagaatta aaagtaccta gcaagtacag tgtagaatgt
     9301 ataatttcta taggatatcc tgatgaagaa aagaaagcat acggtgactc tgatttagac
     9361 tataaaaagg ttcattttaa caacttttag tatataatat gttgtattaa gacattatat
     9421 ttttttctat tgttaatata tacttaaaat tgtaaaaata tatctgaatt ttactaactt
     9481 atctaggggt tatgtacaag gattttaaag ataaatatag aaattaatgt ttataatcga
     9541 tacataactt gagtaacaaa atttaaggag gttaaagtat gggtatattt aaaacaaaaa
     9601 tggatgagga ctggaaagta aactacatta aagagtttaa tgaaatgaga gattcttatg
     9661 agagtaaact tcaaaaaaaa cagtttgagg tagatagttt gaaatcagaa ttggatagac
     9721 taagaagcta taaaaatagc cttaagccaa aagaaaaaca aattactgat gatgatataa
     9781 ataatataaa gagtttaaga agagatggct taagctataa agaaatttca aatcaaacaa
     9841 gttggagcaa agcaactgta agtagggtat taaatggact ttacgattag catatacatg
     9901 gggtgattta aattgggatg aagtatagtt acaaaaaatg cataatagat tctattttat
     9961 tgatgtttat agttcaaata ttaaggatga ttttgaatta tgtacttcta agtcaatttg
    10021 aatttactct tgaaaatttt aatattataa acttaatttc ttttacatta gttggtttat
    10081 cgttaatttt atttttaaaa gataattctt tgtataacaa ggttagaaat agaaaaatca
    10141 cagaagcttt tgaagaaaat aaaaacaata tacttattga aaagtgtaag ctgatacttt
    10201 ttgttgtggt tttatcttta gcaataattg taacttattg tactaaggga tatgtacttt
    10261 ttaatgtaac tatgatgact ttgtctgtat tgatagtccc aatatttgaa gagttatttt
    10321 ttagagaata tatatggaac tatctaagta attttataaa gtcaaagagt aaaattatct
    10381 gtataacatc tatactatct ggaatttata atataggata catagatgtt ataagaaatt
    10441 atgtaatatt gtataacaat tcaagctaca cctttgaagt tattatttca aaaattatga
    10501 taggtactgt ttttggtatt gtacttggat tagttaagta taggtttaga gatgtagggt
    10561 tttgtatatt attgaggagc ttatttgcta tatttattag gtagatagta taatcggttt
    10621 ataaggttgt tttaaattta attttaaatc aaccttattt tatttgtgtt ttataaagca
    10681 cttatatatt aataactaat aaatttaata aaaattattt gaatattttt aaaatatata
    10741 aa
//
LOCUS       DAMJVK010000118        10689 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.123, whole genome shotgun sequence.
ACCESSION   DAMJVK010000118 DAMJVK010000000
VERSION     DAMJVK010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10689
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.123"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            106..453
                     /locus_tag="PC414_003262"
     CDS             106..453
                     /locus_tag="PC414_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDF2465635.1"
                     /translation="MHENKKNLLDGSLAQYNDTAVPKIPVFAGNDITSEVYYFKPNQV
                     LNAHRHPNGEQIFVFLKGEGKMKLGEHECDVKNGDTVFVPTGEWHEITNGSNEEMVAV
                     QITKINAGAEYRG"
     gene            complement(603..1103)
                     /locus_tag="PC414_003263"
                     /pseudo
     CDS             complement(603..1103)
                     /locus_tag="PC414_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897038.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(1130..2635)
                     /locus_tag="PC414_003264"
     CDS             complement(1130..2635)
                     /locus_tag="PC414_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF2465636.1"
                     /translation="MNVAIYLRKSRADEEAEHRGEFETLSRHKTTLLKLAREQNLNVI
                     EIKEELVSGESIEYRPKMLELLGEIKNNNYDAVLVMDIDRLGRGNMQDQGLILDTFKK
                     SKTKIITPRKTYDLNDEFDEEYSEFEAFMARKELKLISRRMQRGKIKSIEEGKFIAQA
                     PPYGYDIKYLNNKERVLVINEDKATVVKIIFDMYLKGDGACKISNYLNELGYKTTTGR
                     TWYEKGIRDIIKNKVYCGYIVWNKVERKRNSSRTRGIEEQIEVKGKHEPIISEDIWNK
                     AQNIRKKRINSPTPNNKITNPLAGIVKCGYCNHTLIAKSNTTSNGYEKYMVCTNCKNK
                     ATKLYILENKILEVLNKWLVNYRLSLKDEKDNSKPENTLEHTLINLNKELDTLEKQKE
                     RLHDFLEQGIYDSNTFGERLKILLSRIENAREAINKTNIDIEMQKERNTVKTQIVPQL
                     EKILSEYTEEKEPYLKNILLKSVINYAVYKKEPNQKSDDFSLILYPNIPRD"
     gene            complement(2834..3688)
                     /locus_tag="PC414_003265"
     CDS             complement(2834..3688)
                     /locus_tag="PC414_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2465637.1"
                     /translation="MEWIERLNKAINYIEENITKEIEYEQVAKIACCSTYHFQRMFAY
                     MADVPLSEYIRRRRMSLAAVELQNDDKKIIDVALKYGYSSPTAFNRAFKSIHGVAPSV
                     IKKREITTLKAFPPISFKISIKGAEEMNYRIEKKEAFRIVGVSQPLHNELEKNFEIVP
                     QMWQKVALDGTLQKLIPMMDSQLQGVLGISICNNSEEWKYFISVSSTKSIDNTLEEYT
                     VPAFTWAIFSGEGQCPQAIQELEKRIVTEWLPTSGYEYDNGPDIELYLNPDPQNAKFE
                     VWIPVVKK"
     gene            4073..4354
                     /locus_tag="PC414_003266"
     CDS             4073..4354
                     /locus_tag="PC414_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465638.1"
                     /translation="MPNIEKDLDGTTTITYLNDDIGVPTDENGYKISMTILHGKTICH
                     VVSPETILGRKFTLEEKQEKDTIIYGAIEKIMVDRQLQIKNSNKEVASH"
     gene            4504..5352
                     /locus_tag="PC414_003267"
     CDS             4504..5352
                     /locus_tag="PC414_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003442793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HDF2465639.1"
                     /translation="MIKSFLMLGQSNMAGRGFISEVTPIYNERIQMLRNGRWQMMTEP
                     INYDRPVSGVSLAASFADAWCCENQEDRIGLIPCAEGGSSLDEWNIDGILFKHAISEA
                     KFAIQSSELTGILWHQGENDSNNGNYKFYYKKLLSIIETLRKELNIPDIPIIIGGLGD
                     FLGKVGFGKSCTEYVFINQELQKFAFEQDNCYFVTATGLTSNPDGIHIDAISQRKFGL
                     RYFEAFHKKKHIMEALANESELIIPSNSKTYTKTEKIYINSMDLALGKISYDEFESKL
                     IKLNDN"
     gene            5404..5925
                     /locus_tag="PC414_003268"
     CDS             5404..5925
                     /locus_tag="PC414_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003442791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2465640.1"
                     /translation="MIPLLILGLLKQNPNSYGYELLALMEKKHYKYIVNFTKGSFYYN
                     LQQLEEKKFIEKVENKQSNKNRETHNYIITELGCQEFEKLMYKYGSKTEYINLSFYAT
                     LLFANDYKNEKLIELLETQIKQTQVRISLLNESIENDKTNSHYFNRMLENSRSHHLVN
                     IEWFEKLLNDIRD"
     gene            6276..6809
                     /locus_tag="PC414_003269"
     CDS             6276..6809
                     /locus_tag="PC414_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HDF2465641.1"
                     /translation="MTEVIIVNDARIISQIKAGKKELFDTLIKKYYDSIYCYCYRHVD
                     NKMTAQDLTQDVFVTVINNIENYKHYGKFENYLYVIAKNKCKDFWKKKQPIYLESLDK
                     EISNQELSDIENKMFIKEALTKLPELEFEVIILRYYHDLRFKDIAKIMESSVSVTKYR
                     MSRALKKLELYLEGGKL"
     gene            6806..7567
                     /locus_tag="PC414_003270"
     CDS             6806..7567
                     /locus_tag="PC414_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465642.1"
                     /translation="MNNKEIKRSLQKYKVPKYDSSALEKTIKTIHELTLSNTYKRMNN
                     KDFFISQIGFIRKRTWIMQFLILCTICIFLFSTSADSNSFYNSLSLISIASPLFVLTN
                     IEELTKVYNSSILEIEFSTKNTLHKIIATRMLIFGISDLLFMIIILVSAKQIIDVNIL
                     YIIIYILVPFNLMCIGCMEIINRNKGKHTNYYCMIYGISLVLLIVTLSNFRDNLYQFE
                     AINQWLIVFLVTAVTLIYEIRKTWRHLCSFENIII"
     gene            7605..8471
                     /locus_tag="PC414_003271"
     CDS             7605..8471
                     /locus_tag="PC414_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2465643.1"
                     /translation="MELSVERLTKQYNNKIIVDKIDFKLNEGIYGFLGENGAGKTTLM
                     RMICGILSPTSGEVKVDGKNNLDMGEDFRDMIGYLPQNFGYYPDFTAKEFMLYIASLK
                     GLMPSYAKRKTQELLEMVGLSEVINKKIRTFSGGMKQRLGFAQTLLNDPKILILDEPT
                     AGLDPKERVRFRNLISEFSKEKIVILSTHIVSDVEYIADKILIMKKGRFVLTGSIEEV
                     TSKIKDKVWKCVVNENEIDYMSDRYCLANIHHSGGVANLRIVSDIKPHDNACNLKPTL
                     EDLYLYCFNEVL"
     gene            8492..9733
                     /locus_tag="PC414_003272"
     CDS             8492..9733
                     /locus_tag="PC414_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2465644.1"
                     /translation="MSTLIKFELKKIIKRKSTIAVILGSLFLSIILFALMPIQEKAND
                     ESGKEYRGLQAIELKKQQSKLNEGYITDKKVKNSIRDYQKMFNNPNNITTDEGGFSNF
                     KPDIYWKFFYPNKAFYNFIIESYSNPNDYNPEIIKSMPLEDGVNFYKFRNEKVNTILN
                     MEYPDKNYTQKEKDFWLSKNKDIEEPYLNGYYQGWNYFLLARDLLIFTIVAVCISTAS
                     VFSGEYQSGADSIILSSKYGKTKVITAKIVSSFLFGTIIFTINTIVSIGILLLSFGID
                     GWNLPLQLTTSSIPYPYTFLQAILLSVLIIYMILILLISFTLLLSSYMKSPFPVLITN
                     IILLFVPIFLKYSETNKLYNQILNVLPSNAISFSFTNYVDYKFGNLIIALPTMIIFTC
                     LVLTVVFLPFIKNNFKKHQVN"
     gene            complement(9957..10529)
                     /locus_tag="PC414_003273"
     CDS             complement(9957..10529)
                     /locus_tag="PC414_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2465645.1"
                     /translation="MFKKIVAIASSIIVSTICLTGCDFNSNDKKGESSSSKNLDKVTI
                     AEVTHSVFYAPQYAAITKGFFEDEGIKIDLINTQGADKTMAALISGEANVGLMGPEAS
                     IYVYNQNKNDYAVNFAQLTKRDGSFLVARKKMPNFSYNDLKGTEILGGRKGGVPLMTF
                     EYVLKEKGLTIGENLKAGNVNVRTDVQKSV"
BASE COUNT         3951 a         1368 c         1466 g         3904 t
ORIGIN      
        1 tttaaaaata aaagaatttt attcctattg tgtaaagtga gaatactatt ttcttatatt
       61 ggtataataa tagtaaatct aatatagaaa agaggtattt ctagtatgca tgaaaataaa
      121 aaaaatctat tagacggaag cttagctcaa tataatgata cagcagttcc aaaaatacca
      181 gtatttgctg gtaacgatat aacttcagaa gtttattatt ttaaacctaa tcaagttctt
      241 aatgctcata gacaccctaa tggtgaacaa atatttgtgt tcctaaaagg agaaggtaaa
      301 atgaaacttg gtgagcatga atgtgatgta aaaaacggcg atactgtatt tgtaccaaca
      361 ggagagtggc atgaaattac taatggaagc aatgaagaaa tggtagccgt tcaaattaca
      421 aaaattaatg ctggagcaga atacagagga tagatatata agtacagtta gtactttaat
      481 atctatttta ttatataaac tttaatctca aatataaaaa gagtctttta aaaggctctt
      541 tttatatttt tgttaatttt cattataaaa acaagaaaat atctagctaa aactaatata
      601 ttttatttaa tattttttat agcattgttt gcaaactcag tatttacgat atctttataa
      661 ggtgcttcct tatctagttc tttagcttct ttcataacag tcatcaattt cttcaaacct
      721 tcctctttta atacaggatt ttgagaccat gcatctattg atttatatct atctacaact
      781 gttgttaaat cttctaaaga tatatcttca aagaaaggtt gcatagcctt agctacttct
      841 ttgctagatg cttgttgaat ccataattga cctttataaa cagcatttgt aaatctttga
      901 attaaatctt tattgttttt catgtatgat ctcgtagtag agtatgctgt aaaaggtatt
      961 tctccagaat cttttcctat agatgtaact atatatccac taccttcttt ttccattgct
     1021 gttgctgtag gttcaaatgc tgttgtaaag tcaccctctc cacctgcaaa agctccagcc
     1081 attacaccaa attggtaatc agtctattta taacatttaa aacatataat taatctcttg
     1141 ggatattagg gtaaagtatt aaagaaaaat catcagattt ttgattaggt tcttttttat
     1201 agactgcgta atttattaca cttttaagca atatattttt tagataaggt tctttttcct
     1261 ctgtatattc acttaaaatt ttttcaagtt gaggaacaat ttgtgtttta actgtatttc
     1321 tttccttttg catttcaata tctatatttg ttttattaat agcttctctt gcattttcta
     1381 tacgagataa gagtattttt aaacgttccc caaatgtatt actgtcataa attccttgtt
     1441 ctaaaaaatc atgtagtcgt tctttttgtt tttctaaagt atccaattct ttatttaaat
     1501 ttatcaaggt atgttctaaa gtattttcag gtttagagtt gtctttttca tcttttaaag
     1561 atagtctata atttactaac catttattta agacctctaa tattttattt tctaatatgt
     1621 atagtttagt agctttattt ttacaattag tacaaaccat atatttctca tatccatttg
     1681 atgtagtatt agatttggct attaaagtat gattacagta tccacatttt acaattccag
     1741 ccaatggatt tgttatttta ttgtttggag taggggaatt aatacgtttt tttcttatat
     1801 tttgtgcctt attccatatg tcttcactta ttattggttc atgctttcct tttacctcaa
     1861 tttgttcctc aattcctctt gttctactag aatttctttt cctctcgact ttattccata
     1921 ctatatatcc acaatacact ttgtttttta taatatctct tatacccttt tcataccaag
     1981 ttctacctgt tgtggtctta taaccaagtt cgtttaaata attggatatt ttacatgcac
     2041 catctccttt taaatacata tcaaagatta ttttaacaac agttgcttta tcttcattta
     2101 ttacaagtac tctttcttta ttatttaaat atttaatatc atacccataa ggaggagctt
     2161 gagctataaa tttaccctct tcaatacttt tgatttttcc tctttgcata cgtctagata
     2221 ttaattttaa ttccttacga gccataaaag cttcaaattc agaatactcc tcatcaaact
     2281 catcatttaa atcatacgtt tttctaggag taataatttt cgttttagat tttttaaatg
     2341 tatctagtat taacccttgg tcttgcatat taccacgacc caaacggtca atatccatca
     2401 ctaaaacagc atcatagtta ttatttttaa tttcacctag taattctagc atcttaggtc
     2461 tatattctat actttcccca gaaacaagtt cttctttaat ttcaatgaca tttaaatttt
     2521 gttctcttgc tagttttaaa agagttgtct tatgtctact tagagtttca aattcacctc
     2581 tatgttctgc ttcttcatca gctctgcttt tgcgtaaata aattgccaca ttcattaaat
     2641 cacacccctt gaatatatta tattcagtaa cagattgatt atatttaaaa cacaaaaata
     2701 aaccttcaaa atacaagata tattttttag gtttattttt atacagtaaa ttttatagta
     2761 tatttaaaag cttgtaatct aagtaaatat ttatactatt aattttatgc tttcttcatt
     2821 ttttaatttt aaactatttt tttactacag gtatccatac ttcaaattta gcattttgag
     2881 ggtctggatt taagtacagt tcaatatcag gaccattatc atattcataa ccagaagtag
     2941 gtaaccattc tgttacaata cgcttttcta gttcttgtat tgcttgtgga cattgtcctt
     3001 ctccagaaaa tattgcccaa gtaaaagctg gaacagtgta ttcttctaat gtattatcta
     3061 tagattttgt acttgaaaca gatataaaat acttccattc ttctgaatta ttacaaatac
     3121 ttatacctaa tacaccttga agttgactat ccatcatagg gattaatttt tgaagcgttc
     3181 catctaaagc aaccttttgc cacatttggg gaacaatttc aaaattcttt tctagttcat
     3241 tatgaagtgg ctgagataca ccaactatac gaaatgcttc tttcttttca attctataat
     3301 tcatttcttc ggctccttta atactaattt taaaactgat tggaggaaag gcttttaaag
     3361 ttgttatttc tctttttttt ataactgatg gggcaacacc atgaatactt ttaaatgcac
     3421 gattgaatgc tgttggagaa gagtatccat attttaaagc cacatcaatt atctttttat
     3481 catcattttg taattctaca gctgctaaag acatccgtct acgacgaata tattcagaaa
     3541 gtggtacatc tgccatataa gcaaacattc tttgaaaatg atatgtagaa caacaagcta
     3601 tttttgccac ttgttcatac tcaatttctt ttgttatgtt ttcttcaata tagttaattg
     3661 ctttgtttaa tctttcaatc cattccataa aatcagctcc ttgtaattta tataatatct
     3721 attttaaaat agttaatcct ctctttctat gcactaatta actagcttaa taaagaatga
     3781 acatgagaaa gtctgtaatt aactacaaaa atgaacaaat agtattgttt ttcacattaa
     3841 agtatatcac aaattagtat ctaattttta tataagataa cagttttaat ttattatgaa
     3901 atttttataa tattttagct tagacattta attatatata acattttatt tatagttaac
     3961 tatttttgaa gtttaccctt aatcaagcaa cacttagtta ttcaattata aataatgatt
     4021 gagaatagga caatttttaa gatacataaa atataaagag gaggggattt aaatgccaaa
     4081 catagaaaaa gacttagatg gtacaactac tataacatac ctcaacgacg atataggtgt
     4141 tccaacagac gagaatggat ataaaatatc tatgacaata ctacatggaa agacaatctg
     4201 ccatgtagta tctcctgaaa caatcttagg aagaaagttc acattagaag aaaaacaaga
     4261 aaaagataca ataatatatg gagcaataga aaaaataatg gtagacagac aattacaaat
     4321 aaaaaattca aacaaagaag ttgctagtca ttagcacttc tttaataagt aaagtaggac
     4381 aagtattttt tataatatta ttgacttata gtctaattag actataatat aaacatagat
     4441 agtctaatta gactactatt aaaaataaat acaataagaa tttaagaaag gataataata
     4501 ataatgatta aatcattttt aatgttagga cagtccaata tggctggaag aggattcata
     4561 agtgaagtga ctccaattta taatgaaaga atacagatgt tacgtaatgg aagatggcaa
     4621 atgatgacag aacctattaa ttatgacaga ccagtttctg gggtaagtct tgctgcatca
     4681 tttgcagatg catggtgctg tgaaaatcaa gaggatagaa ttgggctaat tccttgtgct
     4741 gagggaggca gttccttaga tgaatggaat atagatggta tactttttaa acatgctata
     4801 agtgaagcta aatttgccat acaaagcagt gaattgacag gaattttatg gcatcaggga
     4861 gaaaatgaca gtaataatgg caactataaa ttttattata aaaaattact ttctattatt
     4921 gaaactctta gaaaagaatt aaatattcca gatataccaa ttattattgg tggattggga
     4981 gacttcttag gaaaagtagg ttttggaaaa agttgtactg agtatgtatt tatcaatcaa
     5041 gagttacaga aatttgcttt tgaacaagat aattgttact ttgttacagc aacaggtctt
     5101 acatctaatc ctgatggtat tcatattgat gcaatatctc aaagaaaatt tggattacgt
     5161 tattttgaag cttttcataa gaaaaagcat attatggaag cattagctaa tgaaagtgag
     5221 ttgataatac cgagtaattc caagacatat acaaaaacag aaaaaatata tataaatagt
     5281 atggacttgg cactaggaaa gatatcatat gatgagtttg aatctaaact tattaaactt
     5341 aatgataatt agataaaata ttcattaata tactaataaa tatagattga aagaggtgat
     5401 tttttgatac cattattaat tttaggatta ttaaaacaaa atccaaattc ttatggatat
     5461 gagttattag cattaatgga aaagaagcat tacaagtaca tagtaaattt cactaaaggt
     5521 tctttttatt ataatttaca gcaacttgaa gaaaaaaagt ttattgaaaa agttgaaaat
     5581 aaacaatcta ataaaaatag agaaactcat aactacataa ttactgaact tggatgccag
     5641 gaatttgaaa aattaatgta taagtatggt tctaaaacag agtatataaa tctatcattt
     5701 tacgcaacac tattatttgc aaatgattat aaaaatgaaa agttaataga attattggaa
     5761 acgcaaatta agcaaactca agtaagaatt tctctattaa atgaatccat agaaaatgac
     5821 aaaacaaatt cacattattt taatagaatg ctagaaaatt ctcgttctca ccatttagtt
     5881 aatattgaat ggtttgagaa gttgctaaat gatattagag attgatttta ataataaaaa
     5941 aacatacata tatattttat ataaatgtat gatatataga ttaaaaataa ggcagataat
     6001 tttcaattga atatctgtct ttttgttata taagatgtta aaagaaactt atactatgta
     6061 agtaaaattt ataaatataa agttgttcat tgttgaatta cagacactta agatgaaaag
     6121 tatgacattt gatataataa aattgtttaa gtattaatta tgagtattaa ttaatggtat
     6181 agataatttt aatataactg tatttataaa taattataaa ataataaatt aattttttta
     6241 tactgacttt tttatagtat aaatcgttat atataatgac ggaggtgata atagtgaatg
     6301 atgctcggat tatatcacaa ataaaagcag gaaaaaaaga attgtttgat actctaataa
     6361 aaaaatacta tgatagcatc tactgttatt gttatcgcca tgtagacaat aaaatgactg
     6421 cacaagattt aactcaagat gtatttgtaa cagttataaa taatatagaa aattataaac
     6481 attatggtaa gtttgaaaac tatttgtatg ttattgcaaa aaacaagtgt aaggattttt
     6541 ggaagaaaaa acaaccaata tatttagagt cattagataa ggagatatca aatcaagaat
     6601 tatctgatat agaaaataaa atgtttataa aagaagcttt gactaaatta ccagaattag
     6661 aatttgaagt gataatttta cggtattacc atgatttaag atttaaggat attgctaaaa
     6721 ttatggagtc aagcgtctct gtcacaaagt acaggatgag tagggcatta aaaaaactag
     6781 aattatatct ggaaggaggt aaattatgaa taataaagaa ataaaaagaa gtctacagaa
     6841 gtataaggtt cccaaatatg attcttcagc tcttgaaaag actattaaaa ctatacatga
     6901 gttaacgctt agtaatactt ataaaagaat gaataataaa gactttttta tatctcaaat
     6961 tggttttata agaaaaagaa cttggattat gcaatttcta atactatgta caatatgtat
     7021 atttttattt agtacatcag cagatagtaa ttcattttat aatagtttat ccttgatttc
     7081 tatagcatca ccactttttg tactgacaaa tatagaagaa ttaacaaaag tgtataatag
     7141 tagtatttta gagatagaat tttcaacaaa aaatacatta cataaaatta ttgctactag
     7201 aatgcttata tttggaataa gtgatttatt gtttatgatt attattttgg tatctgcaaa
     7261 acaaatcatt gatgtaaaca ttctgtatat aataatttat atactagttc cttttaacct
     7321 aatgtgtata ggatgtatgg aaattataaa tagaaataaa ggtaagcata caaattatta
     7381 ttgtatgata tacggtatat ctcttgtatt attaatagtt acactgagta actttagaga
     7441 taatctatat caatttgaag caataaatca atggctaatt gtttttctag tcacagcagt
     7501 aacattaata tatgaaataa gaaaaacgtg gagacatctt tgttcatttg aaaatattat
     7561 tatctaagta aaaaataaga atacacagta tcaggaggaa tagtatggag ttaagtgtag
     7621 aacgtttaac aaaacagtat aacaataaaa ttatagtaga taaaattgac tttaaattaa
     7681 atgagggtat atatggattt ttaggagaaa atggagctgg aaaaacaact cttatgagaa
     7741 tgatttgcgg aatattaagt cctacatctg gagaagtaaa ggtggatggt aaaaataatt
     7801 tggatatggg agaagatttt agagatatga ttggatacct tcctcaaaac tttggatact
     7861 acccagattt tactgccaaa gaatttatgc tatatatagc atcattaaaa ggattaatgc
     7921 ctagctatgc aaagagaaaa acacaagaat tattagaaat ggtaggatta tcagaggtaa
     7981 ttaataaaaa aataagaaca ttttctggag gtatgaagca aaggctagga tttgctcaaa
     8041 cactacttaa tgaccccaaa attttaatat tagatgagcc aacagcaggg ctagacccta
     8101 aagaacgagt tagatttcgc aacctaattt cagaattttc taaggaaaag attgttatat
     8161 tgtctactca tattgtgtct gatgtagaat atattgcaga taaaatttta ataatgaaaa
     8221 aaggaagatt tgttttaact ggctcaatag aagaagtaac ttcaaagata aaagataagg
     8281 tatggaaatg tgtggtcaat gagaatgaaa tagattatat gtctgataga tattgtttag
     8341 caaatatcca tcattctggt ggagttgcaa atcttagaat tgtaagtgat ataaagccac
     8401 atgacaatgc atgcaattta aaaccaacat tagaagattt atatttatat tgtttcaacg
     8461 aagtactata aatatacata aggagatagg tatgtctaca ttaattaaat ttgagttaaa
     8521 aaaaataata aaaagaaagt ctacaatagc agttatttta ggaagtttat tcttaagcat
     8581 cattcttttt gcattaatgc caattcagga aaaagctaat gatgaaagtg gaaaagagta
     8641 taggggttta caggctattg agctaaaaaa gcaacaatct aaattaaatg agggatacat
     8701 aacagataaa aaagtaaaaa actcaatcag agattaccaa aaaatgttta ataatccaaa
     8761 taatattaca acagatgaag gtgggttttc aaattttaaa ccagatattt attggaaatt
     8821 cttctatcca aataaagctt tttataattt tataatagag tcatatagta atccaaatga
     8881 ttataatcca gaaataatta aatcaatgcc tttagaagat ggagttaatt tttataaatt
     8941 tagaaatgaa aaagtaaata caatattaaa tatggaatat cctgataaaa attacacaca
     9001 gaaagagaaa gatttttggt tgtctaaaaa taaggatata gaagagcctt atttaaatgg
     9061 atattatcaa ggatggaatt attttctatt ggctagagat ttactaattt ttactatagt
     9121 agctgtctgc ataagtacag cttctgtatt ttcaggagaa taccaatccg gtgctgattc
     9181 aataatatta tcttctaaat atggaaaaac aaaagttata acagctaaaa tagtaagttc
     9241 ctttttattt ggtacgatta tttttacaat aaatacaatt gtgtctattg gtatacttct
     9301 tttatctttt ggaattgatg gatggaattt accattacaa cttacaacca gttcaattcc
     9361 atacccatat acatttttac aggcaatatt actaagtgtg ttaattattt atatgatatt
     9421 aatactatta attagtttta cacttttatt atcatcttat atgaaatcac cttttccagt
     9481 tttaattaca aatataatat tactatttgt accaatattt ttaaaatata gtgaaacaaa
     9541 taagttgtat aatcaaattc tcaatgtttt accatcaaat gctatttcat tttcatttac
     9601 taattatgta gattataaat ttggtaattt aataatagct ttacctacaa tgattatttt
     9661 tacatgctta gttcttacag ttgttttttt accttttatt aaaaataatt ttaaaaaaca
     9721 tcaagttaac taaaatatat attataaaaa gaaggtgtct taaaatgaac tttttagctt
     9781 attaggcact cttctttgta ttaatttaaa tatattttag tattagatta aatatgtttt
     9841 catcatttta acaatgaaaa agagctttat ctctattttg agatagctct tataaagtta
     9901 aatattaaat tgataactat aaaatatgta gtttgaattt ataatttgtg ttataatcat
     9961 actgattttt gtacatcagt tcttacatta acattgcctg cttttagatt ttctccaata
    10021 gtaagacctt tttcttttag aacatattca aaagtcatta gtggaactcc accttttcta
    10081 ccacctaata tttcggttcc ttttaaatca ttgtaagaga aattaggcat ttttttccta
    10141 gcaactagaa aactaccatc tctttttgtc aattgagcaa agttcactgc ataatcgttt
    10201 ttgttttgat tatatacata tatagatgct tcaggaccca ttaaacctac gtttgcttca
    10261 cctgaaatca aagctgccat agttttatct gcaccttggg tatttataag gtcaatcttt
    10321 ataccttcat cttcaaagaa tcctttcgta atagcagcat actgtggagc ataaaaaaca
    10381 gagtgagtaa cttctgctat agttacttta tccagatttt tagacgaaga agattcacct
    10441 tttttgtcat tagagttaaa atcacatcca gttagacata ttgtacttac aatgatagag
    10501 cttgctattg cgactatttt tttaaacata ataaatacct cccataagac atacgaatag
    10561 tatatagtat taaaaattta ttgtagttgt tacaaaaatg tatctatgct tataatattt
    10621 ctacaaaaac ttaataataa ttttcagctt tatgtttagt tttttcagta atgtgtaata
    10681 ttggataaa
//
LOCUS       DAMJVK010000119        10658 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.124, whole genome shotgun sequence.
ACCESSION   DAMJVK010000119 DAMJVK010000000
VERSION     DAMJVK010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10658
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.124"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            134..1294
                     /locus_tag="PC414_003274"
     CDS             134..1294
                     /locus_tag="PC414_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HDF2465646.1"
                     /translation="MIETGFVYCSFLVVFVGVIFFMVDKLKWKVFDVINPMLLIYLGS
                     AILGSFGLFAQNEDVGTYQNIITVNFLPLMLVFLLIQCDLRKILKMGPKMLLSFFCAT
                     FTFMLGFVIAFIIFKNSMGADSWKILGAASGSWIGGSSNMIAAANALGVAEEGLSYAI
                     LMGSIGYTVWMSVCLMSVKFGPKFNKWTKCDTSFIDEVTSKLEKENKDNTAPTFVELM
                     ILLSVGFGVASFAQIVSKILPTSGILNETMWVVLVASTVSILLGMTKISKLKGSTLVG
                     NVFMYLLLAATGSKANFFGLSEAPVYIMFGLVVVLVHAISFIGLAKLFKLDLFTCEVG
                     SIANIGGVTTAPMIAGVHNPVLIPVGVLMGLLGNIIGTYCALMVTQILHMLA"
     gene            1636..1797
                     /locus_tag="PC414_003275"
     CDS             1636..1797
                     /locus_tag="PC414_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465647.1"
                     /translation="MIIPSRRIQDITLTNLKNGEVTLIELDEIYKKMGFLFVVSEGKL
                     KKIKKENHH"
     gene            1990..2619
                     /locus_tag="PC414_003276"
     CDS             1990..2619
                     /locus_tag="PC414_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HDF2465648.1"
                     /translation="MKIVTYNIHKGMDSNNRLTLTKMGLYLKQLDCDVICLQEVLYPQ
                     FLALKAVLNMDGVFATNVKKVNMIYGICTFTTSKMLNSNHFFLTSKKEQRGALCITID
                     AYGRIINVINTHLGLDRQERAKQLDEIIDYRNRLVGIVILCGDFNEKNVFLSMFNDMA
                     ISLNKSYLPTFEKSNSRIDYIFVNKNTELKGYTVEKIYLSDHYPVIGYI"
     gene            2858..4117
                     /locus_tag="PC414_003277"
     CDS             2858..4117
                     /locus_tag="PC414_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HDF2465649.1"
                     /translation="MGRRTTRVSRLGKEKKRSVLIVLAALVLIMGGFTYFFNSKFLYN
                     GKIAKNVYIEGVNVSDMTKAEALKAITDKYTPEDLNLAYDGKKYTISPKDIDLKYDTE
                     DVVKDAYESTKKGSYFQNLKKYIDIRVNKANMKIKAEYDEAKLSSKVSSIADSINVKM
                     KNASISVGSGGLSYTDSVVGREFDLAANKESIYNMIKNKEHKTLELKVNLQKPDITTE
                     QVKTVNSVIGQYSTTYSQAVEGRSYNVGLSARKTSDVLLMPGEEFSYNKLTGPSNKAN
                     GYKDAPVIVYGKLEQSAGGGVCQTSSTVYNAALLSGMEITQVTNHSSASTYVPKGRDA
                     TVSDGGLNLKFKNPYKHPVYIKNYAGGGSVSSVIYGNSGDKPNISIEVKQTGQNKYST
                     YRIFKDSSGKVIKKEHISNSSYKELKK"
     gene            4376..5371
                     /locus_tag="PC414_003278"
     CDS             4376..5371
                     /locus_tag="PC414_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HDF2465650.1"
                     /translation="MYEEVTWLNFIGNRYEVVNSDDVLEINKIYKARDVFYRKNVLIK
                     VIKHNNYICEDFVSNLIDESTALDEINSPYILKIIDVGIHCTEETTLYYVVSEDFNGI
                     GLDELILGNYLHLEAIVNIMIQVLKALEMIHRNHSYHGSLKSSSIIVDTEYNIKICDF
                     GITKANNGVNTRSYGNRRYLCPHQLCINYTDKESDFFATGLILFESIFKKLPFGESNS
                     EEKMLQSIDKGLDWNSIKAINGNTELINVIKRLLGRNNKYTRANDIIIDLSKVMYEKA
                     YIEVEDEKEEEQKQDKVIQVKKEYKSKMRKKLHKKLVVAGLAIVMISMIIISSII"
     gene            5617..6684
                     /locus_tag="PC414_003279"
     CDS             5617..6684
                     /locus_tag="PC414_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HDF2465651.1"
                     /translation="MSNIKIIASNKCWIEDIARKQLEDISKLNGILRVVGLPDLHAGK
                     IPVGLAVETKNIIYPHIIGNDIGCGMTLFKTGVLKKKFKKDKWIKSLSKIKDLSDIEI
                     KNTYKEECPILNLGTIGSGNHFAEIQCISEIYNKEQFEQLKFSSDDIMMLVHCGSRNY
                     GEDILKKFYDKDGLEVESEKAIDYIKKHDNALMWAERNREAISKKIMQSIGTSGDVET
                     IFSINHNFIEKREDKFIHRKGAVSSERGAVIIPGSRGSLSYIVMPTENTEISLYSLSH
                     GAGRKWSRSVCKSRLKSKYSKDTIKQTKFKSQIICNDVNLLFQEAPEAYKNIEQIIES
                     LIEYELIQVVATMKPLITYKG"
     gene            7218..9128
                     /locus_tag="PC414_003280"
     CDS             7218..9128
                     /locus_tag="PC414_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HDF2465652.1"
                     /translation="MNSNTFERLQLNEVKDLIKIHCVSSLGKNLIDKLTPSGNIGVVR
                     RKLAENKEARKIIENSNHIPLEGLFNVGSTIDKIEKGMIIEPVELVNIEDFLRGCRKM
                     KAFMLEKEFYSPTLSSYALNITECKSIEDEINYCIKSNKVDSNASKELKKIRRNIEIT
                     EGKIKDRLNKFITSTVNKKYIQEFIISKRNDRYVIPIKSSYKNEVNGTILDTSSKGNT
                     VFIEPISVSNLSTELTMLKADETIEEYKILSYLTELIFEKISQIKLNIEILSEYDMVF
                     AKAKYSQKIKGITPKINNNGYIKIIKGKHPLLTGDAVPLDFEIGKNYRSLIITGPNAG
                     GKTVTLKTVGLLTLMVQCGFDISAKEGSEFSVFEKVFVDIGDNQSIENALSTFSSHIK
                     NIAEIMSLSNNSTLVLFDEIGSGTEPNEGAGLAISLLEEFYKMGCITIASTHYGEIKK
                     FATLHPEFENAGMMFDKETLEPLYKLTIGKSEDSNALFISKKMGIKNRVLDRAKEYVI
                     DRNYNLDLVKRSKLQSNIEDKNSSEVILNYTDYNVGDKVKLLDEDDFGIVYKPMDKFN
                     NVEVLFNDNFISVNIKRLQLSIKASELYPEGYDLDVLFTSFKERKLEKDIQRGSKKAL
                     KKIQKEIRENRK"
     gene            complement(9191..9814)
                     /gene="ytaF"
                     /locus_tag="PC414_003281"
     CDS             complement(9191..9814)
                     /gene="ytaF"
                     /locus_tag="PC414_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation membrane protein YtaF"
                     /protein_id="HDF2465653.1"
                     /translation="MIESILLVTSLCIDAFVTSFGYGVNKVKVPLYSNIIISVVCSAI
                     LAISLFAGTIVRDFLPQNITMLICFFILFLLGSARLIESFFKSYFKKKSIHSKNYNFN
                     MLDLKLNFNVSIEPDVLDTSKSKVLKSAEAFTLALALSLDGLAIGFGSGLVGVNYIQV
                     ILFSLISNIIAVLLGCLLGNMFVKNVNLNLSWLSGLILLVIGFMKIC"
     gene            10002..10646
                     /locus_tag="PC414_003282"
     CDS             10002..10646
                     /locus_tag="PC414_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2465654.1"
                     /translation="MEQNNKVGNFGDLLKILLDEKELSMGELSKKSGIDKSTISRIAN
                     NKQKPNINHLEKMAIHLNINLEELLKASGYEFRNSNNQIFNADSDFSNFDDILGFANL
                     INDKNFNGNIEKELSKCKLYVQTDEGKKLLFDNFNKKIDSIEKQGQFTDRLRQMYADF
                     CTDGLTIKKYLLIGSMLLYFVISTDVIPDFVFPIGFMDDLVALNIVTKLLKNDK"
BASE COUNT         4360 a         1053 c         1753 g         3492 t
ORIGIN      
        1 agttaggtat atatgtattt aattagcatg atatagataa aagtatttat tttaattgtc
       61 tattatctga aaaagtcttt acttagaaat cataaaaatc aaattaatta ggaaaagaaa
      121 gggtgtgtat aatgtgattg agactggatt tgtatattgt agtttcttgg ttgtattcgt
      181 aggtgtaatt ttttttatgg ttgataaatt gaaatggaag gtatttgatg ttattaatcc
      241 aatgctttta atatacttag gttcagctat acttggatca tttggtttat ttgcacagaa
      301 tgaagatgtt ggaacgtatc aaaacataat aacagtgaac ttccttccat taatgttagt
      361 gtttttgtta attcaatgtg atttaagaaa aatattaaag atggggccta aaatgttatt
      421 atcatttttc tgtgctacat ttacatttat gcttggattt gttatagcat tcataatttt
      481 taaaaattca atgggtgcag attcatggaa aattcttggg gcagcatctg gttcatggat
      541 aggtggctca tcaaatatga ttgcagcagc taatgctcta ggagttgcag aagaaggact
      601 tagttatgca atacttatgg gttctatagg atatacagta tggatgtctg tttgtctaat
      661 gtctgtgaaa tttggaccta aatttaacaa atggacaaag tgcgatacaa gttttataga
      721 tgaggttaca tcaaaacttg aaaaagaaaa taaagataat acagcaccta cgtttgtaga
      781 acttatgatt ttactttctg ttgggtttgg agttgcgtct tttgcacaaa ttgtatccaa
      841 gatacttcca acttcaggta tacttaatga aactatgtgg gtagtacttg ttgcatctac
      901 agtatcaata ttattgggta tgacaaaaat atcaaagctt aaagggagta cactggtagg
      961 aaatgtgttt atgtatttac tccttgcagc tacaggtagt aaagcaaatt tctttggatt
     1021 gtcagaagca cctgtttaca taatgtttgg attggtagta gtccttgtac atgctatttc
     1081 atttataggg cttgctaaac tatttaaact tgatttattt acttgtgaag taggaagtat
     1141 agctaatata ggaggagtaa ctacagctcc aatgattgct ggggttcaca atcctgtatt
     1201 aataccagta ggtgtactta tggggcttct aggaaatatt atagggactt attgtgctct
     1261 tatggtaact caaatattac atatgttagc ttaataaaaa tatggtataa aattaataat
     1321 ttaaataaga aaagtcattt caatttaagc agaagctttt actgaaatgg cttttttgtt
     1381 tgacaagata atctatttca agaagatgtt ttgacatatt tagtgtataa tattgtaaaa
     1441 tttactaaaa acgagatata tttaattttt atattgaatc gaacgtctgt atgatgtata
     1501 ataaatatac agaacgaatg tttgcagaac gggtgttttt tgaatttatt ttaataattt
     1561 atgaatatat atcagaatgc ataaatatat ttaaatataa agattaaaaa gaatttaaag
     1621 gagtgttgat aacttatgat aattccatca agaagaatac aagatataac attaacaaat
     1681 ttaaaaaatg gtgaagtaac tttaattgaa ttggatgaaa tatacaagaa gatgggattt
     1741 ttatttgttg ttagtgaagg caaattgaaa aagataaaaa aggagaatca tcattagaaa
     1801 tgaatgaaat aataaatatt ataatagttt ataaaaccta acttatgtag aatataaata
     1861 cttaagttag gtttttttgt gataaagttt ataagtactc aattttataa gataaataat
     1921 ggtcttaagt gaaaagtaaa taagtttaaa ttttagtaag tattatattg cataagaggt
     1981 gatattatta tgaaaatagt aacctacaat attcataaag gtatggattc aaataataga
     2041 ttaactctaa ctaaaatggg gttatattta aaacaattgg attgtgatgt aatttgtttg
     2101 caagaagtat tatatcctca atttttagct ttaaaagctg ttttaaatat ggatggtgtc
     2161 tttgcgacaa atgtaaaaaa agtaaatatg atatatggta tttgtacatt tacaacatct
     2221 aaaatgttaa acagtaatca ttttttctta actagcaaaa aagaacaacg gggagctctt
     2281 tgtataacta tagatgcata tggaagaatt ataaatgtaa taaatactca cttgggactt
     2341 gataggcagg agagagcaaa acaattagac gaaataatag actataggaa cagattagtg
     2401 gggatagtta ttttatgtgg tgattttaat gagaaaaatg tctttttaag tatgtttaat
     2461 gatatggcta tttcactaaa caagtcttac ttaccaacat ttgaaaaatc aaactctaga
     2521 atagattaca tttttgttaa taaaaatact gaactaaaag gatacacagt agaaaaaatt
     2581 tacctgtcag accattatcc tgtaataggt tacatttaaa tttcaatata aatgaaatta
     2641 gttgttataa aataaacaat tttttgttaa gaaatagtaa aagaaatata ataaaaaata
     2701 tcaaaaataa aactaattat aagacataaa tttacaataa agttacattt gattaaattc
     2761 cttgtttaac aaaagctatt caaatataat ctaaggtagt tttgaaatgg gaactctatt
     2821 atgcaaattt aagctagtgt taaaatgagg tgaagttatg ggcagaagaa caacaagagt
     2881 ttcaagatta ggtaaagaaa aaaaacgaag tgttttaatt gtattggcag ctttagttct
     2941 aataatgggt ggatttactt atttttttaa cagtaaattt ctgtacaatg ggaaaatcgc
     3001 taaaaatgta tatatagaag gagtaaatgt atctgatatg actaaagcag aggcattaaa
     3061 agctataaca gataagtata ctccagaaga tttaaatctt gcttatgatg ggaaaaaata
     3121 tacaatatca ccaaaagata ttgatttaaa atatgataca gaagatgttg taaaagatgc
     3181 ttatgaaagt acaaaaaaag ggtcctattt tcaaaactta aaaaaatata ttgatataag
     3241 agtgaataag gcaaatatga agataaaagc tgaatatgat gaggcaaaac ttagttccaa
     3301 agtgtcttct atagcagata gtataaatgt taagatgaaa aatgcttcta ttagtgttgg
     3361 aagtggtgga ctaagttata cagactctgt tgtaggtaga gaatttgatt tggcagcaaa
     3421 taaagagtct atatataata tgataaaaaa taaagagcat aaaactttag aacttaaagt
     3481 aaatcttcaa aaaccagata taacaactga acaagtaaaa actgtaaatt ctgttatagg
     3541 tcaatattca actacttatt cacaagctgt agagggtagg tcatataatg taggattatc
     3601 agcaagaaaa actagtgatg tattattaat gccaggtgaa gaattttcat acaataaact
     3661 tacaggacct agtaataaag caaatggtta caaagatgca cctgttatag tttatggtaa
     3721 gctagaacaa tcagcaggag gaggtgtttg tcaaacatct tcaacagttt ataatgcggc
     3781 tcttctttcg ggtatggaga taactcaagt tacaaatcat tcatctgcat ctacttatgt
     3841 gcctaaaggg agagatgcta ctgtgagcga tggaggatta aacttaaagt ttaaaaatcc
     3901 atataagcat cctgtatata ttaaaaatta tgcaggtgga ggaagtgtat catcagtaat
     3961 ctatggtaat tctggtgaca aaccaaatat atctatagag gtaaaacaaa ctggtcagaa
     4021 taaatattct acatatcgaa tatttaaaga ttcaagtgga aaggtcataa aaaaagaaca
     4081 tatatcaaat agttcttata aagaattaaa aaaatagata attttttaga gaaatatttg
     4141 taatataaaa gggagtgtct caaaatgaac tttttagttt atgagacact ctttttctgt
     4201 gaaatcaaat atattttcat catttcaaca atacaaaaga agcgtatctc tattttgaga
     4261 cagccttttt tgtaaaaatt aacactatta gttcttgttt taatattaca atcattaata
     4321 tatagaaaat attcctatat attggtataa ttgtaaatag atttagttat ataaaatgta
     4381 tgaggaggtg acatggttga attttatagg caatagatat gaggtagtaa attctgacga
     4441 cgttttagaa attaacaaaa tctataaagc tagagatgtt ttttacagga aaaatgtgtt
     4501 aataaaagtt ataaagcaca ataattatat ctgtgaagat tttgtttcaa atttaattga
     4561 tgaaagtact gctttagatg agataaactc tccttatata ctaaaaataa tagatgttgg
     4621 gattcactgt acagaagaaa caacattata ttatgttgta agtgaagact ttaatggtat
     4681 cggactagat gaattaattc taggtaatta tcttcattta gaagcaattg taaacataat
     4741 gatacaagtt ttaaaagctt tagaaatgat acataggaat cattcatatc atggtagttt
     4801 aaaatcgagt agtataatag tagatacaga atacaatata aaaatttgtg attttggaat
     4861 tacaaaagca aataatgggg ttaatacaag aagctatgga aacaggagat atttatgccc
     4921 acatcaacta tgtataaatt atactgataa agaaagtgat ttttttgcaa caggtcttat
     4981 tctatttgaa tctatattta aaaaattgcc ttttggagaa tcaaattcag aagaaaaaat
     5041 gctacaatct attgataagg gattagattg gaatagtata aaagcaatta atggaaatac
     5101 tgaacttata aatgttataa aaaggcttct tggaagaaat aataagtata ctagagccaa
     5161 tgatattatt attgatttaa gtaaagtaat gtatgaaaaa gcatatatag aagttgaaga
     5221 tgagaaagaa gaagagcaga aacaagacaa agttatacaa gttaaaaaag aatataaaag
     5281 taaaatgaga aaaaaattac ataaaaaact tgttgttgcc ggtctagcta tagtcatgat
     5341 ttcaatgatt attattagtt ccataatata aaaattgtat attaagtatt ttaaaatcaa
     5401 tgtctataaa ggatatacat agaaatttta gtgtagtctt aaattatgaa aggaagtggt
     5461 tattatgtta acaaaaaata atgaggtatt aaacgtctag gagtatttta acaaatagcc
     5521 acctgctttt acagtttaga ataaaaaagc tgtggctaaa ggtcacagct ttttgctact
     5581 ttttaaaggc aggagaatat taaaaaggag caaattatga gcaatataaa aataatagca
     5641 agtaataaat gttggattga agatatagca aggaaacaac ttgaagatat ttcaaagtta
     5701 aatggaattt taagagtagt aggattacct gacttacatg caggtaaaat acctgtaggt
     5761 ttagcagttg agactaaaaa tattatttat ccacatataa ttggaaatga tataggatgc
     5821 ggaatgactt tatttaaaac tggggttcta aaaaagaaat ttaaaaaaga caaatggatt
     5881 aagtcactta gtaagattaa agatttgtct gatatagaaa taaaaaacac atataaagaa
     5941 gaatgtccaa ttttaaattt aggaactata gggagtggaa atcattttgc agaaattcaa
     6001 tgtataagtg aaatttataa taaagaacaa tttgaacaat taaaattttc atctgatgat
     6061 attatgatgt tagttcactg tgggtcaaga aattatggag aagatatttt aaaaaaattt
     6121 tatgataaag atggacttga agttgaaagt gagaaagcta tagattatat taaaaaacat
     6181 gataatgctt taatgtgggc agagcgtaat cgtgaagcta tcagtaaaaa aattatgcag
     6241 tcaataggta catctggaga tgttgaaaca atattctcta taaatcataa ttttatagag
     6301 aaaagggaag ataaatttat acatagaaaa ggtgctgttt caagtgaaag aggagctgtg
     6361 attataccag gttcaagagg cagtctatca tatatagtta tgcctactga aaatacagag
     6421 atatctttat attctctatc acatggagca ggtagaaaat ggtctcgttc agtttgtaaa
     6481 tctcgcctta aaagtaagta tagtaaagat actataaaac aaactaaatt taaaagtcag
     6541 attatatgta atgatgtaaa tttattattt caagaagctc cagaggcata taagaatata
     6601 gagcagatta ttgaaagttt gatagaatat gaacttattc aagtagtggc aactatgaag
     6661 ccattgatta cttataaagg ataattttta aagttaaaaa agattaaaat tatattataa
     6721 atagctaatt ttatggaaca tttaaaagtg ttttattatt agctattttt ttattttgta
     6781 gagacgataa tttatagagc taaattcttt gatatataat gattacaaag ttagactgta
     6841 ttggaaactg cacatacatg aattgatgac aatataagtg attcccaaat ataaatgaaa
     6901 gtataagctt atttttacta taaaaatttt cactgtacaa aaacattaat gacattattt
     6961 ttattgacat aaatatataa tttatggaat aatattagaa gcactgatat aagtgtttaa
     7021 ataagaatag aaaaggagaa gtgttttatg aaaaaaaata ctaacattaa tgagtccgga
     7081 gttaaacttc ttaataaata aggagtttga tatataataa ctccttattt attaagttgt
     7141 tcgcaaaata actattatca aagtaaatga tagggtgatt taaaagacga tgacttataa
     7201 ataaaggaga aaaaataatg aatagtaata catttgaaag attacaacta aatgaagtta
     7261 aagacttaat aaaaatacat tgtgtaagtt cgctaggaaa aaatttaata gataaattaa
     7321 ctcctagtgg gaatattgga gtagtaagaa ggaaattagc agaaaataaa gaagctagaa
     7381 agataattga aaatagtaat cacataccac ttgaaggatt gtttaatgta ggttctacaa
     7441 tagataaaat tgaaaaagga atgataatag aacctgttga acttgtaaat attgaagatt
     7501 ttttaagagg ttgtaggaag atgaaagctt ttatgttaga aaaagaattt tattcaccta
     7561 cattaagttc ttatgcctta aatataacag aatgtaaaag tatagaagat gagataaatt
     7621 actgtataaa atctaataaa gttgattcta atgctagtaa agaattaaaa aagataagac
     7681 gaaatataga aattacagaa ggaaagataa aagatagatt aaataaattt ataacatcaa
     7741 ctgtaaataa aaagtatata caagagttta taataagtaa aagaaatgat agatatgtaa
     7801 taccaattaa atcttcttat aaaaatgaag ttaatggtac tatattagat acatcatcaa
     7861 aaggaaatac tgtatttatt gaacctatta gtgtttcaaa tttaagtaca gagttaacta
     7921 tgctaaaagc tgatgaaact attgaagaat acaaaatatt atcttattta acagaactta
     7981 tatttgagaa gataagtcag ataaaattaa atatagagat actttcagaa tacgatatgg
     8041 tatttgcaaa agctaagtat agtcaaaaaa taaaaggaat tactccaaaa ataaataata
     8101 atggctatat aaaaataatt aaaggtaaac atccacttct aacaggagat gcagtacctt
     8161 tagattttga aataggcaaa aattatagaa gtcttataat aacaggtcct aatgcaggag
     8221 gaaaaactgt aacccttaag actgttggtt tactaacttt aatggttcaa tgtggatttg
     8281 atatttctgc aaaagaaggt agtgaattta gtgtatttga aaaagttttt gtagatatag
     8341 gggataatca aagtattgaa aatgccttaa gtactttttc atcacatata aaaaatattg
     8401 cagagattat gagtttatca aataattcta ctcttgttct gtttgatgaa ataggaagtg
     8461 gaactgaacc taatgaagga gctggtttag caatatcatt attagaagag ttttacaaaa
     8521 tgggatgtat aactatagca tcaactcatt atggagagat aaaaaagttt gctacattgc
     8581 atccagaatt tgaaaatgct ggtatgatgt ttgataaaga aacgttagaa ccattgtata
     8641 agttaactat tggaaaatct gaagatagta atgctctttt tatttctaaa aaaatgggaa
     8701 taaaaaatag agttttagat agagctaaag aatatgtaat tgatagaaac tacaatttag
     8761 atttagttaa aagaagtaag ctacaatcta atattgaaga taaaaatagt tctgaagtca
     8821 ttttaaacta tacagattat aatgtagggg ataaggtaaa attactagat gaagatgatt
     8881 ttggaattgt atataaacct atggacaagt ttaataatgt agaagtattg tttaatgata
     8941 attttatttc agtaaatata aaaagattac aacttagcat aaaagcatca gaattgtatc
     9001 cagaagggta tgatttagat gtattattta ctagttttaa agaaagaaaa ttagaaaaag
     9061 atattcaaag aggttcaaaa aaagctctta aaaagattca gaaagaaatt agagagaata
     9121 gaaaataaat ataaaaagca caaaagaaag caatctaaag agaaaagttt atctttagat
     9181 tgctttctta ttaacatatc ttcataaatc caatgactaa caagattaaa ccacttaacc
     9241 aagataagtt taaatttacg tttttaacaa acatatttcc tagtaaacaa ccaagtaata
     9301 ctgcaattat atttgatatt aaagaaaata gaataacttg tatataattc acacctacaa
     9361 ggccactacc aaaaccgata gctaatccat ctaaacttaa agcaagtgca agtgtaaatg
     9421 cttcagcaga tttaagcact ttagatttac ttgtatctaa gacatcaggt tcgatactta
     9481 cattaaaatt taatttaagg tctaacatat taaaattata attctttgag tgtatagatt
     9541 ttttcttaaa ataagattta aaaaaacttt caattagtct agcacttcct aataaaaata
     9601 aaataaagaa acatattaac attgttatat tttgaggtaa aaagtctcta actattgtac
     9661 ctgcaaaaag cgatattgct aaaatagctg aacaaactac gctgataatt atatttgaat
     9721 ataagggaac tttaacttta tttacgccat atccaaagct tgtcacaaag gcatctatac
     9781 aaagagaagt aactaataaa attgattcaa tcacagtaga ttaacatcct ttctcttaaa
     9841 ttttgtactt atattagtta tagtattgta aaaatggata aattgttaca gataatccaa
     9901 taaattgttg ataaaattcc aacatcatac aacacctaaa taacaaaaga atcatatcat
     9961 ataataatat aaaaccaatc ataataaagc gggtgaaaat aatggaacaa aataataagg
    10021 ttgggaattt cggtgatttg ttgaagattt tattagatga aaaagaatta tcaatgggag
    10081 aactaagtaa aaaaagtgga attgataaat ctactatatc tcgtatagct aataataaac
    10141 aaaagccaaa tataaatcat ttagaaaaga tggcaataca tttaaatatt aacctagaag
    10201 aactgttgaa agcttcagga tatgaattta gaaacagcaa taatcaaata tttaatgcag
    10261 atagtgattt tagtaacttt gatgatattc ttggatttgc aaatttaatc aatgataaga
    10321 actttaatgg aaatattgaa aaagagttaa gtaagtgtaa gttatatgtc caaacagatg
    10381 aaggtaaaaa attacttttt gataatttta ataaaaaaat agattctatt gaaaaacaag
    10441 gtcaatttac agatagatta agacaaatgt atgctgattt ttgtacagat ggacttacaa
    10501 taaagaaata tttattgatt ggaagtatgc tactttattt tgttatatca acagatgtga
    10561 ttccagactt cgtattccca ataggattta tggatgattt agtagcatta aatatagtta
    10621 caaaattact taaaaatgat aagtaagttt tagaatca
//
LOCUS       DAMJVK010000120        10345 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.125, whole genome shotgun sequence.
ACCESSION   DAMJVK010000120 DAMJVK010000000
VERSION     DAMJVK010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10345
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.125"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            286..1716
                     /locus_tag="PC414_003283"
     CDS             286..1716
                     /locus_tag="PC414_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2465655.1"
                     /translation="MSSQESKPKGNLISNFRDDMRDPKKRAIYTYSSSINLAYNFIGY
                     FVSTVTNIFLTDVAKLGVIMAGGMQIIQSFIKIAVAPITGTVFDKQPFKKGKYYPWLR
                     YAPAALALTYGIFFIVPLMKISPALLLPLMTALLLIASCAQQVVFTIIYAIYPVVAKT
                     PRDRVIGSTTTNIFKEVGKFLVGFSYPLLLVLFADVLGGEGMGYLGTYLFFAAGCLLV
                     FWFSSTEIIKSGAEEEVLQKEEKPKIKASEMAKALFTNVPLMMAFCLEFLICIRSIGL
                     GPLAPYYFKYVIEDERGLAVFLSIMPLVSVAFMFFAPVFIKICREQKLASIISFSICA
                     LCHLLVAWTPWGKTTLGVTMLLAIGGGFSNVVSIINLNFFAGSCDYGHWKSGKDLPGL
                     SMSLYPVAIQVGVLLATTIRTVLMNSMGYQADMVVTEAVKSGFINMISYSMAIPLIIA
                     VVIAILYPVSDKKLNQIREELNQRNA"
     gene            1819..2922
                     /locus_tag="PC414_003284"
     CDS             1819..2922
                     /locus_tag="PC414_003284"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HDF2465656.1"
                     /translation="MIEKLLRNTVKELHQYVPGEPIEKVKEKYGVKEIIKLASNENPL
                     GPSPKAIEAMIEMLKQGQLYPEPEANELRRKLAEKLDLKPENFIVANGADNVITLIGE
                     AFINRGDEVIYCNPTFPSYRTATIKNEGIPVEVPLTEDYKYDLQGILDKITDKTKLIC
                     VCNPNNPTGTIVDDKELEEFLKKVPENIITILDEAYIEFLTVPNYVDGLKYVRENYNV
                     IVTRTFSKIYGLAGLRVGYGIAKDEIIRTLFTVKEPFSANRVAISGATAALDDEEFIK
                     ETYELNRVGMAYFKEEFTKMGFDVVDSQSNFLYVDMKTDIPKLFEDLKKRGFVIRPSA
                     THARVSIGTMEENRKFVAVLKEILNIKGEPSIG"
     gene            2937..3173
                     /locus_tag="PC414_003285"
     CDS             2937..3173
                     /locus_tag="PC414_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2465657.1"
                     /translation="MKRGVDMNLKINAERCKSCEYCVISCKKGALKISSKINKEGYAH
                     VEVDESKCILCGICYQVCPDNVFEIIKEEACQEA"
     gene            3190..4239
                     /gene="vorB"
                     /locus_tag="PC414_003286"
     CDS             3190..4239
                     /gene="vorB"
                     /locus_tag="PC414_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HDF2465658.1"
                     /translation="MKKMWKGNHAIAEAALRGGCEFYAGYPITPQTEVMEFLSHRMSE
                     LGRTFIQSENEMAAIYMVYGAYASGMRSMTSSSGPGISLKQEGISYLCANHYPCVIVN
                     VQRWGPGLGSLDSAQTDYLRDTRGGGNGDYHLIVYAPNSIQETVDLMYNSFDVAEKYR
                     VPVEILTEAALGQMMEPVEFPEFKKREGDLGWTYDGSNRDHAKVADNQKPTFCMEKRM
                     RITENEQQWEDYQVEDAEYVFVAFGIPSRTTMNAVRRLREQGEKVGIIRPITVWPFPY
                     KAFEKVSKDVKGFISVESTDTGQLVEDVALASKKVCKENVPVYGLFSGNHIPKTLQVM
                     DTYSKIKSGEIKEVF"
     gene            4241..4981
                     /locus_tag="PC414_003287"
     CDS             4241..4981
                     /locus_tag="PC414_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HDF2465659.1"
                     /translation="MATEKLTPSVVSIPNKFCPGCGHGIVNRIIAEVIEEHGYEKNHV
                     LTLGVGCVCNMNFSWNGDKMQTAHGRASSTAIGVKVALPDTLVMTYQGDGDAYVIGLS
                     ETLNTAYRNHNVTVFVINNNNFAMTGGQMSWTTMPGQVTTTSVNGRDIKTTGSPLKVP
                     EMVANFFDVAYVARGSVHSPKEIINLKKYIKNAIEAQLNGEGYSLVEILAPCPTNWGV
                     SLEKSIKWMEEEIVPYYALGEFKQRDGE"
     gene            4984..5523
                     /locus_tag="PC414_003288"
     CDS             4984..5523
                     /locus_tag="PC414_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HDF2465660.1"
                     /translation="MEKELVFAGFGGQGVLTLGLIVAESALELGKQVTWMPAYGPTMR
                     GGKAYSVVKFSDESIGGPDMEEIDVLVAMNKPSLDYINLVKEGGTVVINTSAIDENVT
                     LREDISVVKINCQELAQKVNNPKAANIVVLGALIAKTGLFEKELALKTMCDFFEEKGK
                     GKFNVQNEAAFMEGYNNAI"
     gene            5647..7746
                     /locus_tag="PC414_003289"
     CDS             5647..7746
                     /locus_tag="PC414_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HDF2465661.1"
                     /translation="MSLEKLIRPKSIAIVGVTDKLGFGRSAALSIVKSKETDRVYYVN
                     PKREELFGRKCYKTIQEVPEVVDCVVVCTPRNVVPSVLKDSGELGTKAAVVYASGFAE
                     EGTEEGTDLENQLIEISNTYDMKILGPNCMGLLNCIDKVNLWAGGSKWDLDTKKPGIG
                     IVGQSGFITAEIVSSDYFNISYGFSTGNGNIVTLEDAVDFLVDDDYASVIAIYLEGLK
                     NPQKFIDALKRAAQKRKPVIILKSGRSEKGAISAASHTGNLAGSSKAFESIFEKYGVI
                     SVENLEQFMCLAQAFSVLDGNLPANSNFAAINFSGGENTICADLAEENGVELAEISSE
                     TKEEMEKYLPGFATPKNPLDATTALFHEKDMIVGLLHTFEKDDSVGFTMVGANIRDEE
                     NEMHETLCKAVSEAREQGLKKPVFAVPTLEGNRYLDYRTRLEDNQVPIMSSVGTTFTC
                     FNKMAKFIDYDYSKRTLEFKAVKKRESNNVVALSELDSKIEMKKYGVPVPGQGNAKSI
                     DELDEILKYIKYPVVLKINSSEILHKSDVGGVKIGIKNRDEAVDAYNEILTNVKKAKP
                     DANIDGILVQEMVESGIEIIIGITNDDQFGPMLLVGLGGVFVEVFKDTTLYPLPINHD
                     EAIMMLKKLKSFKLLNGYRGSEPCDIDALADMMVKLGKYAYENKDEVKEIDLNPVFVY
                     PKGKGVCAVDALIVKYK"
     gene            complement(8013..8528)
                     /locus_tag="PC414_003290"
     CDS             complement(8013..8528)
                     /locus_tag="PC414_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HDF2465662.1"
                     /translation="MISAKMEKLLNEQINHELYSAYLYLSMSSYLEAEGLKGFSNWFY
                     VQYKEETDHAMFFYKYLHNVGGKVQLDAIPMPDSDFTSAMDILERTLAHEKKVTALIN
                     NLAAVANSESDFRTSQFLLWFISEQAEEETNCEDNIKRVKLAGEGGLFFVDQEFANRT
                     YAAPTNPPVTI"
     gene            complement(8620..9831)
                     /locus_tag="PC414_003291"
     CDS             complement(8620..9831)
                     /locus_tag="PC414_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HDF2465663.1"
                     /translation="MSNKQGSNIKDVKIKNKLIILKLLSTNTPMSRVDLAKSTGLTKM
                     TLSNLVTELINENMIREIESSTLNAHSSGRKPILLDISNISPCICGILIRRNICKVII
                     SDLKGNIVKQLSETYPKFLSNDVLKDIIKRMFTKIMKINKRDIIGIGISSIGPVDDIN
                     GTILNPPDFGNVSNLNIVDFIKEMSDLPIFLINDANSGALAEKMYGLGKNISNYIYLH
                     IMNGIGAGLVLENKLHTGNLGQSGEIGHTSINFSGPLCSCGNNGCLDYYTSVSNLIKR
                     IESLSHIYPNSPLINCKDFSLVKLIDEANNKDSLAMFLLDEFCTYVSYALVNTLTLID
                     CSSIIIGYDFNIPGTFIENTLLKKLTASASFSKYKKISVRHSNFGANAPLIGSIAIVS
                     YNFFNGSINFY"
BASE COUNT         3921 a         1255 c         1880 g         3289 t
ORIGIN      
        1 tgataaaata atttaaaata cgcatttcaa tgacatttca aaacaagaaa ataataaaaa
       61 acaaacctaa attaagtcta aatagctttt tagttaattt tagtagtaaa aaataaaact
      121 taaaaagtgt ttgacaatta tgaattttga gaatataatt aaattaaaat tatacacata
      181 taaaaatata ctcctgaatc aagaatgaga tatttaagat tcaaaatata tattattata
      241 cgtgaataat aaaaagtaag taaattttat aagggagggg tagatgtgag tagtcaagaa
      301 agcaaaccaa aaggaaatct tatatcaaat tttagagatg atatgagaga ccctaagaaa
      361 agagcaatat acacatattc aagttctata aatttagcgt acaattttat aggttacttt
      421 gtatcaactg ttacaaatat ttttttaact gatgtggcaa aacttggtgt aattatggca
      481 ggtggtatgc aaattataca atctttcata aaaattgctg tagctcctat aacaggtaca
      541 gtatttgaca aacaaccatt caaaaaagga aagtattatc catggcttag atatgcacca
      601 gcagcgttag cattaacata tggaatattt ttcatagttc cattaatgaa aataagtcca
      661 gcattacttt tacctttgat gacagcttta ttattaatag catcttgtgc tcagcaagta
      721 gttttcacta taatttatgc tatttaccct gtagttgcta agacacctag agaccgagtt
      781 ataggctcaa ctactacgaa tatatttaaa gaagttggta aattcttagt aggtttttca
      841 tatccgttat tattagtttt atttgcagat gtattaggtg gagaaggaat gggttattta
      901 ggtacctact tattctttgc agcaggatgc cttttagtat tttggttttc ttcaacagag
      961 ataataaaat ctggagcaga agaagaagtt cttcaaaaag aagagaagcc aaagattaaa
     1021 gcaagtgaaa tggcaaaagc tttattcact aatgtaccac ttatgatggc attttgttta
     1081 gaatttctta tatgtataag atctatagga ttaggacctt tggctccata ttattttaaa
     1141 tatgtaatag aagatgaacg tggattagca gtatttttat caatcatgcc tttggtaagt
     1201 gtagcattca tgtttttcgc accagtgttt ataaaaatct gtagagagca aaaactagct
     1261 tcaataattt ctttttctat atgtgcacta tgccacttat tggttgcatg gactccttgg
     1321 gggaaaacta ctttaggagt aactatgttg ttggcaattg gtggaggttt ttcaaatgta
     1381 gtatcaataa taaatcttaa tttctttgcc ggttcttgtg attatgggca ttggaaatca
     1441 ggaaaagact tacctggctt aagtatgtca ctatatccag ttgcaataca agtaggagta
     1501 ttacttgcaa ctactataag aacagtttta atgaactcaa tgggatatca agctgatatg
     1561 gtagtcacag aagcagttaa atcaggtttt ataaatatga tttcttattc tatggcaata
     1621 cctttaataa tagctgttgt tatagcaata ctatatccag taagtgacaa gaaattaaac
     1681 caaataagag aagagttaaa tcaaagaaat gcttagttaa aagaaagttg agtagatggt
     1741 taatttaagc tattaaagta agtagacgca atagcgttta tataaatgaa aatacatata
     1801 cagagggggc ttttaaaaat gatagaaaaa ttattgagaa ataccgtaaa agaattacat
     1861 caatatgttc caggtgaacc aatagaaaaa gttaaggaaa aatatggtgt aaaagaaata
     1921 ataaaattag cttcaaatga aaatcctcta ggaccatcac caaaagcaat agaagctatg
     1981 attgaaatgt taaaacaagg tcaactttat ccagagccag aagctaacga acttagaaga
     2041 aaattagctg aaaagctaga tttaaaacca gaaaatttta ttgttgctaa tggtgcagat
     2101 aacgttatca ctctaatagg tgaggctttt ataaatagag gagatgaagt tatatattgt
     2161 aacccaacat ttccatcata tagaactgca actataaaaa atgaagggat accagtagaa
     2221 gtacctttaa cagaagatta taagtatgat ttacaaggta tattagataa gattacagat
     2281 aaaacaaaat taatatgtgt atgtaaccct aataatccaa caggaacaat tgtggatgat
     2341 aaagagttag aagaattttt aaagaaagtc cctgaaaata ttataactat attggatgaa
     2401 gcatatatag aatttttaac agttcctaat tatgtggatg gacttaagta tgtaagggaa
     2461 aattataacg ttatagtaac aagaactttc tctaaaatat atggtcttgc agggcttaga
     2521 gtaggatatg gtattgctaa agatgagata ataagaactt tatttacagt aaaagagcct
     2581 ttctctgcaa atcgtgttgc tataagtgga gcaacagcag cacttgatga tgaagagttt
     2641 ataaaagaaa catatgaatt aaatagagtt ggaatggctt actttaaaga agagtttaca
     2701 aaaatgggat ttgatgttgt agattcacaa tcaaatttcc tttatgtaga tatgaaaaca
     2761 gatataccta agttatttga agacttgaaa aaaagagggt ttgtaataag accaagtgct
     2821 acacatgcac gtgtaagtat tggaactatg gaagaaaata ggaaatttgt agcagtttta
     2881 aaggaaatat taaatattaa aggagagcca agtattggat aatagataga aatattatga
     2941 aacggggtgt agatatgaac ttaaaaataa acgcagagag atgtaaaagt tgtgaatact
     3001 gcgttatatc ttgtaaaaaa ggagcattaa aaatatcaag taagataaat aaagaaggtt
     3061 atgctcatgt agaagttgat gagagtaaat gcatactttg tggaatttgc tatcaggtgt
     3121 gtccagataa tgtttttgaa ataattaaag aagaagcatg tcaagaggct tagaaggagt
     3181 tgggataata tgaaaaagat gtggaagggg aaccacgcca ttgcagaagc agccctaaga
     3241 ggaggttgtg aattttatgc aggatatcca attacaccac aaacagaggt aatggaattt
     3301 ttatcacata gaatgtctga acttggaaga acttttatac agtccgaaaa tgagatggct
     3361 gctatatata tggtatatgg agcatatgca agtggaatga ggtctatgac gagttcttca
     3421 ggaccaggaa tatctttaaa acaagaaggg atttcttact tatgtgcaaa tcattaccct
     3481 tgtgtaatcg taaatgtaca gagatggggg cctggtcttg gctcattgga ctcagcacag
     3541 acagactatc taagagatac tagaggggga ggaaatggag attatcattt gatagtttat
     3601 gcaccaaaca gtattcaaga gactgtagat ttgatgtata actcatttga tgtagcggag
     3661 aagtacagag tgcctgtcga aattttgaca gaagcagctt taggtcaaat gatggaacca
     3721 gtagaatttc cagagtttaa gaagagagaa ggagatttag gttggactta tgatggaagt
     3781 aatagagacc atgcaaaggt tgcagataac caaaagccaa ctttttgtat ggagaaacgt
     3841 atgagaataa ctgaaaatga acaacaatgg gaagattatc aagtagaaga tgcagaatat
     3901 gtgtttgtag cgtttggtat accatcaaga acaactatga atgctgttag aagacttaga
     3961 gaacaaggtg aaaaagttgg aataataaga ccaataacag tatggccttt cccatataaa
     4021 gcatttgaaa aggtaagtaa agatgtaaaa ggatttatct cagtagaatc aacagacaca
     4081 ggtcaattag tagaagatgt tgctttagcc tcaaagaaag tatgtaaaga aaatgtacct
     4141 gtgtatggtt tatttagtgg aaatcatata ccaaaaacat tgcaagtaat ggacacttat
     4201 agcaaaataa aatctggcga aatcaaggag gtgttctaat atggctacag aaaaattaac
     4261 acctagcgtt gtttcaatac caaacaagtt ttgcccaggt tgtggtcatg gaatagtaaa
     4321 tcgtataata gcagaggtaa tagaagaaca tggatatgaa aaaaatcatg tattaacatt
     4381 aggagttggt tgtgtctgca atatgaattt tagctggaat ggtgataaga tgcaaactgc
     4441 acatggtaga gcttcatcta cagctatagg agttaaagta gcacttccag atacattagt
     4501 aatgacttac caaggtgatg gagatgctta tgttataggt ctttctgaaa ctttaaatac
     4561 agcatataga aatcataatg tgactgtatt tgtaataaac aataataatt ttgcaatgac
     4621 tggtggacaa atgtcatgga ccactatgcc gggtcaagtc acaacaacaa gtgtaaatgg
     4681 aagagatata aagactactg gtagtccact gaaagttcct gagatggtgg caaacttctt
     4741 tgatgtagca tatgtagcaa gaggttcagt tcatagccca aaggaaataa ttaatctaaa
     4801 aaaatatata aaaaatgcaa tagaagctca attaaatgga gagggatatt ccttagttga
     4861 aatattagca ccttgcccaa ctaactgggg tgtatcacta gagaaatcta tcaaatggat
     4921 ggaagaagaa atagtgccat attatgctct tggcgaattt aagcaaaggg atggtgaata
     4981 gttatggaaa aagagctagt atttgcaggg tttggtggtc aaggagtttt aactcttggt
     5041 ctgattgtag ctgagtcagc acttgaattg gggaaacaag taacttggat gcctgcatat
     5101 ggtccaacta tgagaggtgg aaaagcctat agtgtagtaa agttttctga tgagagtata
     5161 ggtgggcctg atatggaaga aatagatgtt ttagtagcaa tgaataaacc atctttagat
     5221 tatataaatt tagttaaaga aggtggaaca gtagttatta atacatctgc aatagatgag
     5281 aatgtaacat taagagaaga tataagtgta gttaaaataa attgtcagga attagctcaa
     5341 aaagtaaaca atccaaaagc agcaaatata gtagtacttg gagcattaat tgctaaaact
     5401 gggttatttg aaaaagaatt agctttaaaa actatgtgtg atttctttga ggaaaaagga
     5461 aaaggaaagt ttaatgttca aaatgaagca gcattcatgg aaggctataa taacgccatt
     5521 tgataaatta cccttatctt aagtgattta tctcaagatt taataatctt tagtgccaag
     5581 agaaacttca tcttgagata aaattcactt aaaatcacaa ctataaacat ataggagcgt
     5641 gaatttatga gtttggaaaa acttattaga ccaaaaagta ttgctatagt aggagtgact
     5701 gataaacttg gttttggaag aagtgcagca cttagtatag taaaaagtaa ggaaacagat
     5761 agagtatatt atgttaatcc caaaagagaa gaattgtttg ggagaaaatg ttataagacg
     5821 atacaagaag ttccagaagt agtagactgt gtagtagtat gtacgcctag aaatgtagtt
     5881 ccatctgtat taaaagattc tggagagtta ggaacaaagg cagcagtggt atatgctagt
     5941 ggatttgcag aggaaggtac agaggaaggt acagatttag aaaatcaact tatagaaata
     6001 tcaaatacat acgatatgaa aatattgggt ccaaactgca tggggttgct aaattgtata
     6061 gataaggtta atctatgggc aggaggttct aaatgggatt tagatactaa aaaacctggt
     6121 ataggaattg taggtcaaag tggatttata actgctgaaa ttgtttcaag tgattatttt
     6181 aatatatctt atggtttttc aacaggaaat ggaaatatag ttacactaga agatgcagta
     6241 gatttccttg tagatgatga ttatgcatca gtaatagcaa tttaccttga aggtttaaaa
     6301 aatcctcaaa aatttataga tgctttaaaa agggcagcac aaaaaagaaa gccagtaata
     6361 atattgaaat caggaaggtc agaaaaaggt gctatttctg ctgcttctca tacaggaaat
     6421 ttagctggtt cgagtaaagc ttttgaaagt atttttgaaa aatatggagt aatttctgtg
     6481 gaaaatttag aacagtttat gtgtttagca caagctttta gtgttttaga tggtaattta
     6541 cctgcaaatt ctaattttgc agcaataaac ttctcaggag gagaaaatac tatttgtgca
     6601 gatttagcag aagaaaatgg tgtagaactt gcagaaatat cttctgaaac taaagaagaa
     6661 atggaaaaat atcttccagg atttgcaaca cctaaaaatc cactagatgc tacaacagca
     6721 ttattccatg aaaaggatat gatagtaggt ttattacata cctttgaaaa ggatgatagt
     6781 gttggattta ctatggtagg tgcaaatata agagatgaag aaaatgaaat gcatgaaacc
     6841 ctttgtaaag ctgtaagtga agctagagaa caaggattga aaaaacctgt gtttgcagtt
     6901 cctacacttg aaggaaatag atatttagat tataggacta gattagaaga taatcaagtg
     6961 cctataatgt catcagttgg aactacattt acttgtttta ataaaatggc taagtttata
     7021 gattatgatt attcaaaaag aactttagaa tttaaagctg ttaagaaaag agagtcaaat
     7081 aatgtagtag cattatcaga attagatagt aagattgaga tgaaaaaata tggagtacca
     7141 gttccaggac aaggtaatgc aaaaagtatt gatgaattag atgaaatctt aaaatatatt
     7201 aagtatcctg ttgttttaaa aataaattct tctgaaatat tgcacaaatc tgatgttgga
     7261 ggagttaaaa taggaattaa gaatagagat gaggcagtag atgcatataa tgagatttta
     7321 actaatgtaa aaaaagcaaa accagatgct aatattgatg gtatattagt tcaagaaatg
     7381 gtagaaagtg gtatagaaat tattattggt ataactaatg atgaccaatt tggaccaatg
     7441 ttattagtcg gtttaggagg agtatttgta gaggtattta aagatactac attatatcca
     7501 ttacctataa atcatgatga agctattatg atgcttaaaa aactaaaatc atttaagctt
     7561 ttaaatggct atagaggaag tgagccatgt gatattgatg ctcttgcaga tatgatggtc
     7621 aagttaggaa aatatgctta tgaaaataag gatgaagtca aggaaataga cttaaatcca
     7681 gtgtttgtat atcctaaagg aaaaggcgta tgtgcagtag atgccttaat agttaaatat
     7741 aaatagttta taagttttgg tattcaacca taattaaagc accaactgaa aatcacattt
     7801 tgtaagttag atagtgtgat tttcagtcac cccaaattta atttaaaaat attttaaaat
     7861 gaaagtatag ttttaagttt cattgtgaaa tagcactgta taaacaataa aatactacgt
     7921 ataaaacgaa taataaaagg tgttttaatg ataagaatca atcttgattt tcattaaaac
     7981 accttcttta ttatattaaa aatttttaaa cgttatattg tcacaggtgg gtttgttgga
     8041 gctgcatatg tacgattagc aaattcttgg tcaacaaaga atagcccacc ctctccagca
     8101 agtttaacac gttttatatt gtcttcacaa tttgtttctt cttctgcttg ttcagatata
     8161 aaccaaagta agaattgaga agttctaaag tctgattcag agtttgctac agctgctaag
     8221 ttgtttatta aagctgtaac ctttttttca tgagctaacg tacgctcaag aatatccatt
     8281 gcagaagtaa agtctgaatc tggcattggg atagcatcaa gctgaacctt tcctcctaca
     8341 ttatgtaagt atttgtaaaa aaacatagcg tggtctgtct cttctttata ttgaacataa
     8401 aaccaattag aaaatccttt aagtccttct gcttcaaggt aagaagacat agaaagataa
     8461 agatacgcag aatataattc atggttaatc tgctcattaa gtaatttttc cattttagct
     8521 gaaatcattt ttatacctcc aaattttaaa tgctcttata aaatataata atattaaatg
     8581 gaaaaatatt caataaaagt gtttaaatat tttacatatt taataaaaat ttatactacc
     8641 attaaaaaaa ttataactta caatggctat agagccaatt aatggtgcat ttgctccaaa
     8701 atttgaatgc cttacactta tttttttata tttagaaaaa cttgctgaag cagtcaattt
     8761 ttttaataat gtattttcta taaatgtacc aggtatatta aagtcatagc ctattattat
     8821 agaactacaa tcaatcaatg taagtgtatt aactaatgca tatgatacgt atgtacaaaa
     8881 ttcatcaagc aaaaacatgg caagactatc tttattattt gcctcatcta ttaacttgac
     8941 taaagaaaaa tctttacagt taattaatgg ggagttagga taaatatggc tcaatgattc
     9001 aattctttta attagattac tgacacttgt ataataatct aaacatccat tgttaccaca
     9061 actgcacaat ggtccactaa aattaattga tgtatgtcct atttctccac tttgtcctag
     9121 gttgccagta tgaagtttat tttctagaac tagaccagct ccaataccat tcattatgtg
     9181 taggtatata taatttgata tatttttacc taaaccatac attttttctg ctaatgcacc
     9241 cgaattagca tcatttatca aaaatattgg caaatcagac atttctttta taaaatctac
     9301 tatatttagg tttgatacat ttccaaaatc tgggggattt agtatggtac catttatatc
     9361 atctactgga cctattgaag aaataccaat tccaattata tctctcttgt tgattttcat
     9421 tattttagta aacattcttt ttattatatc ttttaaaaca tcatttgata gaaatttagg
     9481 gtaagtttca gaaagttgtt ttacaatatt accttttaaa tctgaaataa ttaccttaca
     9541 tatatttctt cttattagga ttccacaaat acaaggcgat atattagaaa tatctaataa
     9601 aataggttta cgacctgatg aatgtgcatt taaagttgat gactctattt ccctaatcat
     9661 attttcatta ataagttcag taactagatt tgataaagtc attttagtaa gtcctgtaga
     9721 cttagctaaa tcaactctgg acataggtgt atttgtagaa agtaatttca atattattaa
     9781 cttattttta atttttacat ctttgatgtt agaaccttgt ttgttactca tcataaatct
     9841 ccttaaataa agttaagtgt tttatttatt ttcaagtata ctatcacctt ataatagagt
     9901 aacatttttt tacttgaaaa tatcattaat tagaatatat cacattatat atcataaaac
     9961 atataaaaaa atattagaat taaattaaaa gtgaaaagta gattgatata taaatagtaa
    10021 atacttaatt aaaaaaagat aatgatagca ataattattg ttaccaagaa tctactactt
    10081 caagtgttac cttaggtatt taagttgata gtggtttgca cgtaataaac ttgatgtaat
    10141 atatattctt aataatatcg ataaaagatt tacttaaaaa taatatagat tatgtaaaca
    10201 tataatctat ttattgtata caataaaatt tcattttgta aaacagtgaa ttaaatatgt
    10261 aatatagata tcgattttta ttacaaattg ttactaaata taggtacatt agaacaaaaa
    10321 tctagtaaat tgtaactaaa atgta
//
LOCUS       DAMJVK010000121        10294 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.126, whole genome shotgun sequence.
ACCESSION   DAMJVK010000121 DAMJVK010000000
VERSION     DAMJVK010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10294
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.126"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            196..1620
                     /locus_tag="PC414_003292"
     CDS             196..1620
                     /locus_tag="PC414_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HDF2465664.1"
                     /translation="MSSSNQSTIKTSIQRVGKFLSGMVMPNIGAFIAWGLITALFIPT
                     GWMPNEKLSTIGDPMIKYLLPLLIAYTGGKAIAGQRGGVIGAAAAMGVIVGADIPMFI
                     GAMIMGPFAGWVIKKFDKFVDGKIPTGFEMLVNNFSIGIIGMLLAILGFYAIGPAIVA
                     GTALIESGVQFIVSKSLLPLVSVFIEPGKVLFLNNAINHGILGPIGIAEAKEAGKSIM
                     FLLESNPGPGLGVLLAYWMFSRGSVKQSAPGAVIIHFFGGIHEIYFPYILMNPVLILA
                     TMAGGAAGILTFSILGAGLVAAPSPGSIFALMALAPKGGLLPVLAGVAVATVVSFLVA
                     APFVKRASANQSEEDSTSLEEAKAKMSDMKSASKNSEKNIEEKQLEVNEIKKIVFACD
                     AGMGSSAMGASRFKNRIKNLDLNIEITNSSVDNLPDDTQIVVTHNTLVERVAKNNSSV
                     EIVSINNFLNDPNLDTLFKRLESK"
     gene            1655..3784
                     /locus_tag="PC414_003293"
     CDS             1655..3784
                     /locus_tag="PC414_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDF2465665.1"
                     /translation="MKKKKITSRQKKIILMIVANSKKNIPITISEIAGTLELSSRTVL
                     RDMSGIEKWFDENDFNFVKKPGVGLILEENIENQNFIIELLEEEKIEKEYSKEERNLI
                     ILSKLLVSNEPVKSYYFAKILKVSEWVLNNDFALASKWLERFDIELVRKPGLGVYLKG
                     QEKNFREAYVNLIYDSFNEKEILDMVRNISENIQTDKAIEILSENRLLNLMDRCIIRK
                     VEATLTKKLSDLDVNLADSAYIGLVVHISLALQRIKNGENITMDKEFLKELSITEEFK
                     LAKEIVKGMETDFNMDIPVDEVGYITMHIRGAKQRSSSNHKALNLDDIEIMEITNKMI
                     DLAEDEFKISLKNDERLFKDLANHLGPSINRLNMGLEIRNPLLDEIKSKYSYAYDVVE
                     KISRIIKDKLNINSIPESEIGYIAMHFASAIEKNLMMNTNINIVVACPTGIGTSRFLS
                     TKIENKFPNLNILETISAINIDEEYLKEKDVDLIVSTVELNTSLNYICVGPFMSLDDE
                     QIIKEKIKSIAQNKLINLNAKNDTKSKNKVYEQITESMNIGKDILQFLEEIRFEKFES
                     KDLSELIEDSSKIFAKSSEDIISIKESLKERLKISIPYIEESKILLLHCMSERIDIMK
                     LSIIKLENRIVLDSNEEIDNVVFMLLPKNSPSYQRQIMSEISGSLIDNIIFTNKINKF
                     SIEEMALEVKDIVFNFYTNRLKAFIDK"
     gene            3814..4236
                     /locus_tag="PC414_003294"
     CDS             3814..4236
                     /locus_tag="PC414_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2465666.1"
                     /translation="MSKVLNENNIFLGLDSVSKEEAITLAGRKLVENGYVKEEYIPAM
                     LEREKVMTTYMGMGVAIPHGVNEAKKEILSSGIVILQFPNGIDFDGEKAYLLIGIAGV
                     GDEHLEILSNIAVVLDDDLTERLKNSNDKQAFMEAFAN"
     gene            4255..5406
                     /locus_tag="PC414_003295"
     CDS             4255..5406
                     /locus_tag="PC414_003295"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDF2465667.1"
                     /translation="MKKAIQFGAGNIGRGFIGGLLVKSGYHVVFADVNEEILNSINKD
                     KKYTIHIRDVECIDEVIDNISAVSSIKEEIIDEIVQAEIITTAVGPLVLTKIASTIAK
                     GIKARKEKGLTSNLNIIACENAIYASSSLKEEVLKYLNKEEVEYLEMYVGFPNCSVDR
                     IVPPGKNENPLDVTVENFYEWNVEKQGFKGEIPTIVGMNLADNLMAYIERKLFTLNTG
                     HAITAYIGYLKGYKTIEESIKDKFICDIVKSAMVESGEGLIKKYNFDSEVHYKYIDKI
                     LNRFKNPYLNDDVLRVGREPLRKLSDKDRLIKPLMTAKSYGLSVDNLILGIGAALHYN
                     NSEDTQSVELQELIKSIGVKKAVAKIANISNDEELLNNIEKSYIFMKNL"
     regulatory      5512..5613
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            5796..6368
                     /locus_tag="PC414_003296"
     CDS             5796..6368
                     /locus_tag="PC414_003296"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDF2465668.1"
                     /translation="MKALQEKILREGSVSGNDILKVDSFLNHQIDVAFLNEIGREFKE
                     RFKGEKVDKIFTIEASGIAIASIVSQYFDNAPVVFAKKSESKNLDKDVYETNVYSFTK
                     AREYSVKVSKKYINKGENILIVDDFLANGRAALGLKDLIEQAEANLVGVGIVIEKGFQ
                     AGGALLKANDVRLESLAVVESIDNGTVKFR"
     gene            6432..7082
                     /gene="fsa"
                     /locus_tag="PC414_003297"
     CDS             6432..7082
                     /gene="fsa"
                     /locus_tag="PC414_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDF2465669.1"
                     /translation="MRFFIDTANIEEIKEANDLGVICGVTTNPSLIAKEGRDFIEVVK
                     EISEIVDGPISAEVISLEHKGMIEEAEKLSKIHKNIVIKIPMTAEGLKAVKVLSSKGI
                     KTNVTLIFSAGQALLAARAGATYVSPFVGRLDDISQNGLDLIEEIVDIFSVNSIEAQI
                     IVASVRNPIHVLQAARMGADIATVPLKVINQMIKHPLTDKGIDSFMKDWEGASLKL"
     gene            7234..10158
                     /locus_tag="PC414_003298"
     CDS             7234..10158
                     /locus_tag="PC414_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2465670.1"
                     /translation="MDKILREIQREDKKNPYTDQELAEMLNVARSEVISVRKKNNILD
                     SRERRKKILIKDISKIVSENPQMSERKITEILIENGYNISRSAVSKLLKEENLNQSKI
                     GIKDKDPASSGKYVKVSKVVEEDGFEELIGIKGSLKEKVNLAKSAIMYPPNGLHTIIY
                     GETGVGKSELATCMYKYAIKNNIKEENSPFIVFNCADYAENPNLLIAQLFGVVKGAYT
                     GADANREGLVEQANNGILFLDEIHRLPAAGQEILFSLIDRGEFRRLGESSSNRKANVL
                     IISATTENPDSNLLQTFRRRIPMVINLPNMSERPKSERYEIIIKFFEREAKRVNKNFI
                     ITKEVMCALMNYKCTGNIGQLKSDIQVTCARAFSKSIFSSDKVIIDLDSLRDYIKSGY
                     YDCNCKNEKYSEFSVEDIYIDISELVSGKNRILSDNEISEIYNYAEKELKVLESKCFS
                     EEELKNAFIEKLDKKFNAIKNNKNLSDRRSRIDWGGQLKESTLEIMDKVIVFIKEQFK
                     HVNQGLYLALAIHIEHAIGRIKDGKTIINPSLDKIKASMPMEYELSRYIMGIVEDLTQ
                     VTFPEDELGYLAYYINKFCYNEESIKDKVKVVIVTHGKVGIEMSKVVNHILGIECTLG
                     IEIALTDSPSEGIEHVLEELQKIEARKGILVLIDMGSLVILGDEVEKRLGIRCKTVNR
                     VDTLLAMEAGKLATIEGKSLDGIIADLKKNKNYAMINTNKFSYRKNEYGKKNVIITLC
                     LSGVGTALNLKEHIEKQIKEYDTLIEVKPVAFLNNNLEEELNDIESEYNIIAICGTID
                     IDYKNVPFISYNEVLGKNGINKVLEHLNSKQPALNIENQLNNLIHEDLILYNFEGISK
                     EYIIDTLVSKLEEGGYVDSKYILSVYKREAMGSVVMASKVAVPHGLPENVMKPAIAIA
                     RLNKPIVWDNKFMVDLVVLLALKENNKKEIRSLFSKINDQHTLEVLLNTEDKNEIKEL
                     LS"
BASE COUNT         4216 a         1007 c         1940 g         3131 t
ORIGIN      
        1 ttatttacaa aatcatatac tccaaaatac tacataaata aaattaaaca aaaactaaaa
       61 tgacaaatgt cattatctaa agtgacatag aaacggtttc ttaaagttgt tactataaaa
      121 tataaactct ataataaaag tataacaaaa gggaatcata tcgatataga atataattca
      181 agaggaggta aaattatgag tagttctaat caatcgacaa taaagacatc tatacagcga
      241 gtagggaaat ttttaagtgg tatggtaatg ccaaatatag gagcttttat agcatggggt
      301 cttataacag cattatttat accaacagga tggatgccta atgaaaaatt gagtactata
      361 ggtgacccaa tgataaaata ccttttacca ctattgatag cttatactgg tggtaaagca
      421 atagcaggac aaagaggtgg cgtaataggt gcagcagcag ctatgggagt aattgttggt
      481 gcagatatac caatgtttat tggagcaatg ataatgggac catttgcagg atgggtaatt
      541 aagaagtttg ataaatttgt agatggaaag ataccaacag gatttgaaat gttagtaaat
      601 aacttttcaa taggtataat aggtatgctt cttgcaatac taggatttta tgcaattgga
      661 ccagcaatag tagcagggac agctcttata gaatctggag ttcaatttat agttagcaaa
      721 agtttattgc ctctagtttc agtatttata gaacctggaa aagtattatt cttaaacaat
      781 gcaataaatc atggtatatt agggcctata ggtatagctg aggcaaaaga agcaggtaaa
      841 tctataatgt tcttactaga aagtaaccct ggaccaggtt taggagttct attagcatat
      901 tggatgttta gtagaggaag tgtaaaacaa tcagcaccag gagctgtaat aatacatttc
      961 tttggtggta tccatgaaat ttacttccca tatattttaa tgaatccagt tttaatattg
     1021 gctacaatgg caggtggagc agcaggaata ttgacttttt caatattagg agctgggctt
     1081 gtagcagcac catcaccagg aagtatattt gcacttatgg cactagctcc aaagggagga
     1141 ttacttccag tattagcagg agttgcagtg gcaacagttg tatcattctt agtggcagca
     1201 ccatttgtta agagagcttc agcaaatcaa agtgaagaag acagtacttc tttagaagaa
     1261 gctaaagcaa aaatgagtga tatgaaatct gcaagtaaga actctgagaa aaatattgag
     1321 gaaaaacagc ttgaagtaaa tgagattaag aagattgtat ttgcatgtga tgcaggaatg
     1381 ggttcgagtg caatgggagc ttccagattt aaaaatagaa taaaaaactt agacttaaat
     1441 atagaaatta caaattcatc tgttgataat cttccagatg atacacaaat agtagtaact
     1501 cacaatacat tagttgaaag agttgctaag aataattcaa gtgttgagat agtttcaata
     1561 aacaattttt taaatgaccc aaatttagat acattgttta aaagattaga atctaaataa
     1621 aaataaaaag taccactaat taggagtgga taatttgaaa aaaaagaaga ttacatctag
     1681 gcaaaaaaag ataatactaa tgatagtagc aaattcaaag aaaaacattc caataactat
     1741 ttctgagatt gcagggactt tagagttaag ttcaagaact gtacttagag atatgtctgg
     1801 aattgaaaaa tggtttgatg aaaatgattt taactttgta aaaaaaccag gagtgggatt
     1861 gattttagag gaaaatatag aaaatcaaaa ctttataatt gagttgttag aagaagaaaa
     1921 aatagaaaaa gaatacagca aggaagaaag aaatttaatt atattatcaa agctactagt
     1981 ttctaacgag ccagtaaaat cctattattt tgcaaaaata ttaaaggtat ctgaatgggt
     2041 actgaataat gattttgcct tagcaagtaa atggttggaa agatttgata ttgagcttgt
     2101 tagaaaacca gggctcggtg tctacttaaa aggtcaagaa aaaaacttta gagaggcata
     2161 tgtaaatctt atatacgatt cattcaatga aaaagaaata ctagatatgg tcagaaatat
     2221 aagtgaaaat atacaaacag ataaagcaat agaaatttta agtgaaaata gacttttaaa
     2281 tttaatggac aggtgcataa taagaaaagt tgaagctaca ttaacaaaga agctttcaga
     2341 tttagatgta aatttagctg atagtgcata tatagggctt gtagttcata tttcattagc
     2401 cttacagagg ataaaaaatg gtgaaaatat aactatggat aaagagtttc taaaagaatt
     2461 atctataaca gaagagttta aattagctaa agaaatcgta aaaggtatgg aaacagattt
     2521 taacatggat ataccagtag atgaggttgg atatataact atgcatataa gaggtgcaaa
     2581 gcaaaggtca tcttcaaacc ataaggcttt aaacttagat gatatagaaa taatggaaat
     2641 tacaaataag atgatagatt tggcggaaga tgaatttaaa ataagtttaa aaaatgacga
     2701 gaggttattt aaagacctag caaatcactt agggccttct ataaataggt tgaatatggg
     2761 attagaaata agaaatcctc ttttagatga aataaaaagc aagtattcat atgcctatga
     2821 tgtagttgaa aagatttcta gaattataaa agataaacta aatataaatt caatacctga
     2881 atcagaaata ggatatattg caatgcactt tgcatcagcc atagaaaaaa atcttatgat
     2941 gaatactaat ataaatattg ttgtggcatg tcccactgga ataggtactt caagattctt
     3001 atcaactaag attgaaaata aatttcctaa tttaaatata cttgaaacca tatcagctat
     3061 aaacatagat gaagagtatt taaaggaaaa agatgttgat ttaatagtgt ctacagttga
     3121 gttaaataca agtttaaatt atatatgtgt aggtccattt atgagtttag atgatgaaca
     3181 gataatcaag gaaaaaataa aatctatagc tcaaaataaa ttaatcaatt taaatgcgaa
     3241 aaatgatact aagagtaaga ataaagtata tgaacagata acagaaagta tgaatatagg
     3301 aaaagatatt cttcaatttt tagaggaaat tagatttgaa aaatttgaaa gtaaagattt
     3361 aagtgagctt attgaagact catcaaaaat atttgcaaaa tctagtgaag atataatctc
     3421 tataaaagaa tcgttaaaag aaagattaaa aatttcaatt ccatatatag aagaatcaaa
     3481 aattctattg ttacattgta tgagtgaaag aatagatatt atgaagttat caataataaa
     3541 actagagaat agaatcgtgc tagattcaaa tgaagaaatc gacaatgtag tttttatgtt
     3601 acttcctaaa aattcaccaa gttatcaaag acaaataatg agtgaaataa gtggttcatt
     3661 aatagacaat ataattttta ctaataagat aaataaattc tctatagaag agatggcatt
     3721 agaggtaaaa gatatagttt ttaattttta tacaaacaga ttaaaagcat ttatagataa
     3781 ataaaattaa tattaaaaag gaaggttgat actatgagta aagtattgaa tgagaataat
     3841 atatttttag ggttagatag tgtttcaaag gaagaagcta taactttagc aggaagaaaa
     3901 ttagttgaaa atggatatgt aaaagaagaa tatataccag caatgttaga aagagaaaaa
     3961 gtaatgacta catatatggg aatgggagtt gctattccac atggtgtaaa tgaagcaaaa
     4021 aaagaaatat tatcttctgg aatagttata ttacaattcc ctaatggtat agattttgat
     4081 ggagaaaaag catatttatt aataggaata gctggtgttg gagatgaaca tttagaaata
     4141 ctatctaata tagcagtagt tttagatgat gacttaacag aaagactaaa aaattcaaat
     4201 gacaaacaag cttttatgga agcttttgcc aactaatctt aggagggatt taaaatgaaa
     4261 aaggcaattc agtttggagc aggaaatatt ggaagaggtt ttattggagg cttgttagtt
     4321 aagtcgggtt atcatgtagt atttgctgat gtaaatgagg agatattaaa ctcaataaac
     4381 aaagataaaa agtatactat acacataaga gatgtagaat gtatagatga agtaattgat
     4441 aatataagtg cggtttcatc tataaaagaa gaaataatag atgagattgt acaggctgaa
     4501 ataataacta cagcagttgg acctttagtt ttgactaaaa tagcctctac aattgcaaaa
     4561 ggtataaagg ctagaaaaga aaagggatta acaagtaatc taaatataat agcatgtgaa
     4621 aatgcaatat acgcaagttc ttctttaaaa gaagaggtac ttaagtattt aaataaggaa
     4681 gaagtagaat atttagagat gtatgttggt tttccaaact gttcagttga tagaatagtt
     4741 cctcctggta aaaacgaaaa tcctcttgat gtaacagttg aaaattttta tgagtggaat
     4801 gttgaaaaac aaggatttaa aggggaaata ccaactatag ttggtatgaa tttagctgat
     4861 aatcttatgg cttatataga acgtaaattg tttacactga atacaggtca tgctataaca
     4921 gcttatatag gatatttaaa aggatataag actatagaag agagtataaa agataaattt
     4981 atctgtgata tagtcaaaag tgcaatggta gaaagtggag aaggattgat taaaaaatac
     5041 aattttgact cagaagtgca ctataaatat attgataaaa ttttaaatag atttaaaaat
     5101 ccatatctta atgatgatgt attaagagtc ggaagggaac cattaagaaa actaagtgat
     5161 aaagatagat tgattaaacc tttaatgaca gcaaaatcat atggattatc agttgataat
     5221 ttgattcttg gaataggtgc agcgcttcat tataataata gtgaagatac tcaaagtgta
     5281 gaattacaag agttgataaa gtctattgga gtaaaaaaag ccgttgctaa aatagctaat
     5341 ataagcaatg acgaagaact tttaaacaat atcgaaaaaa gttatatatt tatgaaaaat
     5401 ttataaattt acttgaaaaa aagtagttct gataaagtaa ctataatata ttattctaca
     5461 cagtttgaat tatttgttga ataatagaga attataatat ataattaaaa ggaataaatt
     5521 aatatattta cctgtatatg tttgataata tggttcaaat gtttctacta agctaccatt
     5581 aatagcttga ctacaggagt gaagaagata tagtttatac ttattttaaa ttatatatta
     5641 agttaattac tttgaatttt aagctcctgt ctatgatata gacaggagct tttttagtat
     5701 tttaatataa aatacttaca agaatagata gcaaaattag aacaaatata atttttttat
     5761 taatataatt ttagaaaggg ctaggtggaa gttcaatgaa ggctttacaa gaaaagatat
     5821 tgagagaagg cagtgtttca ggaaatgata tactaaaagt tgatagtttt ttaaaccatc
     5881 aaattgatgt agctttttta aatgaaatag gaagagaatt taaagagaga tttaaaggag
     5941 aaaaggtgga taagatattt acaatagagg cttcaggaat agctatagca tcaattgttt
     6001 cacagtattt tgataatgca cctgtagtat ttgcaaaaaa atcagaatct aaaaatttgg
     6061 ataaagatgt atatgaaact aatgtatatt catttactaa ggcaagagag tattcagtta
     6121 aagtttctaa aaagtacata aacaaaggcg aaaatatatt gatagtggat gattttttgg
     6181 caaatggaag agctgctctt ggacttaaag atttaattga gcaagcagaa gctaacttag
     6241 ttggagtagg aattgtcatt gaaaaaggat ttcaagctgg aggagcactt ttaaaggcta
     6301 atgatgtaag attagagtct ttggcagtag ttgaatctat agataatgga actgtaaaat
     6361 ttaggtaatt tatgatatta tatacttgtg tttagcacat attttaacta tgtaattatg
     6421 gaggattttt tatgagattt tttatagaca cagccaatat tgaagaaata aaagaagcaa
     6481 atgatttagg tgtaatctgt ggtgtaacta ctaatccgtc tctaatagct aaagaaggta
     6541 gagattttat agaggtagtt aaagaaataa gtgaaatagt agatggacca ataagtgcag
     6601 aagtaataag tttagaacat aaaggtatga tagaggaagc agaaaaatta tctaagatac
     6661 ataaaaatat agttataaag atacctatga cagcagaagg tctaaaagcg gtgaaagtac
     6721 tatcaagtaa aggtataaag actaatgtaa cgcttatatt ctctgctgga caagctcttt
     6781 tagctgctag agctggagct acatatgtaa gtccttttgt tggtagattg gatgatatat
     6841 cacaaaatgg tcttgattta attgaagaaa tcgttgatat atttagtgta aatagtattg
     6901 aggctcaaat aatagttgct agtgttagaa atccaataca tgttttacaa gctgctagaa
     6961 tgggagcaga tatagcaaca gttcctctta aagttataaa ccaaatgatt aagcatcctt
     7021 tgacagacaa aggaatagat agctttatga aggattggga aggtgcttct ttaaaattat
     7081 aattataaac tacgctttat aagtatatta aagaggttat tatggaaatt ctaagtaata
     7141 aggctaggct attttgccta gtcttatttt ggtgtataat atattgaaac atattattga
     7201 aatagattaa ttttttaagt gaggaggcga aaaatggata aaatattaag agagatacaa
     7261 agagaagata aaaaaaatcc atatactgac caagaacttg ctgaaatgtt aaatgtagct
     7321 aggtctgaag tgatatcagt taggaaaaaa aataatattt tagattcgag agaaagaaga
     7381 aaaaaaatat taataaaaga tatatcaaaa attgtaagtg aaaatcctca aatgtctgaa
     7441 agaaaaataa ctgaaatatt aatagaaaat ggatataata tatcgagaag tgcagtttca
     7501 aaattattaa aagaggaaaa tttaaatcag tcaaagattg ggattaagga taaagaccca
     7561 gcatcatcag gaaagtatgt gaaggtgagt aaagttgtag aagaagatgg atttgaagaa
     7621 ttgataggta taaagggaag tttgaaagaa aaggtaaatc tagctaagtc agcaataatg
     7681 tatcctccta atggccttca tactataata tatggagaaa caggagtagg taaaagtgaa
     7741 ttagctacat gtatgtataa atatgctata aaaaacaaca ttaaagaaga gaacagccca
     7801 tttatagtgt ttaactgtgc agactatgca gaaaatccta atctattgat agctcaactc
     7861 tttggggtag taaaaggtgc atatacagga gcagatgcaa atagagaagg tcttgtagaa
     7921 caagctaata atggaatatt gtttttagat gaaattcata gattgcctgc tgctgggcaa
     7981 gaaatcttat tttcattaat agatagagga gaatttagaa ggttaggaga aagtagttct
     8041 aatagaaagg caaatgttct cataataagt gctacaacag aaaatccaga ttctaattta
     8101 cttcaaacat ttagaagacg tataccaatg gtaataaatt tacctaatat gagtgaaaga
     8161 ccaaaatctg aaagatatga aataataata aagttttttg aaagagaagc aaagcgagta
     8221 aacaagaatt ttataataac taaagaagtt atgtgtgcac ttatgaatta taagtgtact
     8281 ggaaatatag gtcagttaaa gagtgatata caagtaactt gtgctagagc ttttagtaaa
     8341 tcgatatttt ctagcgataa agtaattata gatttagata gtttaaggga ttatataaaa
     8401 agtggatatt atgattgtaa ttgtaaaaat gaaaagtatt ctgagttttc agttgaggat
     8461 atatatatag atattagtga attggtgagt ggaaaaaata gaattttatc tgataatgag
     8521 ataagcgaga tatataatta tgcagaaaaa gagcttaagg ttttggaaag taagtgcttt
     8581 tcagaagaag agttaaaaaa tgcatttata gaaaagttag ataagaagtt taatgctatt
     8641 aaaaacaata aaaatttatc tgatagaaga agtcgaatag actggggagg acaattaaaa
     8701 gagtctacac tggagataat ggataaggta attgtattta ttaaggagca gtttaagcat
     8761 gtaaatcaag gtctgtactt ggctctagca attcatattg agcatgctat aggtaggata
     8821 aaagatggaa aaactataat aaatccgagt ttagataaaa taaaagcatc aatgccaatg
     8881 gaatatgagt tgtcaagata tataatgggt atagtagaag atttgacaca agtaacattt
     8941 ccagaagatg agcttggata tttggcttat tatataaata aattttgtta taatgaggaa
     9001 agtataaaag ataaagttaa agtagtaata gttacgcatg gtaaagttgg aattgaaatg
     9061 tctaaagttg taaatcatat tttaggtata gaatgtacac taggtataga aatagcactt
     9121 acagattcac catcagaggg gatagagcat gtattagaag aacttcaaaa gatagaagct
     9181 agaaaaggta ttctagttct aattgatatg ggttctttgg taattcttgg tgatgaggtt
     9241 gaaaaaaggc ttggaattag atgtaaaact gtaaatagag tagatacatt attggcaatg
     9301 gaagcaggaa aacttgcaac aattgaagga aaaagtttag atggtataat agctgattta
     9361 aagaaaaata agaattatgc tatgataaat acaaataaat ttagttacag aaaaaacgag
     9421 tatggaaaga aaaatgtaat cattacttta tgtttgagtg gtgtaggtac agctttaaat
     9481 ttaaaagagc atatagaaaa acaaattaag gaatatgaca ctttaattga agtaaagcca
     9541 gttgcttttt taaataataa tttagaagaa gagttaaatg atattgaatc tgaatataat
     9601 attattgcta tctgtggaac tattgatata gattataaaa acgtgccttt tatttcttat
     9661 aatgaggtac ttggtaaaaa tggtattaat aaagtattag agcatttaaa tagtaaacaa
     9721 ccagctttaa atattgaaaa ccagttgaat aatttaatac atgaagattt gattttatac
     9781 aattttgaag gaatttcaaa ggagtatata attgatactc ttgtttcaaa acttgaagaa
     9841 ggtggatatg ttgactcaaa gtatatatta agtgtatata aaagagaagc aatgggaagt
     9901 gttgtaatgg cctctaaggt agccgtgcca catggacttc cagaaaacgt tatgaagcct
     9961 gcaatagcaa tcgcgaggtt gaataaacct atagtatggg acaataaatt tatggtggat
    10021 ttggtggtac tactggcact taaagaaaat aataagaaag aaatacgaag cttattttca
    10081 aagattaatg accagcatac tttagaagtt ttattaaata ctgaagataa aaatgaaata
    10141 aaagaacttt taagctaatt attagtaccg agaagaaaga caaaaaaatt tgctttagta
    10201 taaaatttaa aactactcaa ttgctaagta gttaattact ctatataagg ctagttctat
    10261 gaaatttatt tatatgattt tatagaatta gctt
//
LOCUS       DAMJVK010000122        10257 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.127, whole genome shotgun sequence.
ACCESSION   DAMJVK010000122 DAMJVK010000000
VERSION     DAMJVK010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10257
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.127"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            384..1544
                     /locus_tag="PC414_003299"
     CDS             384..1544
                     /locus_tag="PC414_003299"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HDF2465671.1"
                     /translation="MKKEMLAMILAGGQGSRLGVFTKRIAKPAVSFGGKYRIIDFVLS
                     NCSNSGIDTVGVLTQYRPLILNSHIGMGSHWDLDRINGGVYVLQPFMNEKEGNWYNGT
                     AHAIYQNMDFVDTYNPEYVLILSGDHIYKMDYSKMLKFHKEKGSKATIAVIEVPWDEA
                     SRFGIMNTNEDSSIYEFEEKPSEPKSNLASMGVYIFDWKMLRNYFKEAEKNPEINYDD
                     FGKNLIPKMLEDNVGMYAYPFKGYWRDVGTIQSLWDANMDIIKSPETLDLADPKWKIY
                     TNTMAMPPQYIGKNANVHRSMIADGCRILGEVGNSVLSHGVVVGKGSKVIDSVIMPNV
                     VIGENVTIEKAMIGECATINDNVQIKNVNNEINVVSEYENIEPRCVLIEGGL"
     gene            1546..2676
                     /gene="glgD"
                     /locus_tag="PC414_003300"
     CDS             1546..2676
                     /gene="glgD"
                     /locus_tag="PC414_003300"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HDF2465672.1"
                     /translation="MRNECLGIINLNKKGDPAINKLNYGRPIASTPIAGRYRIIDFAL
                     SNMINSGITKVGIFAKEKYRSLTDHIGSGKDWDLSRKKGGLSIFSPENTKYRNTYSHR
                     EGDIYTILANLDYIEKSEEEYILIAPSYMLCNLNYSQALEYHKKSHNDITIIYKNVNN
                     ANKDFAGNLTLNLDSNNRVINVGNNLGKFPRANICMETYIMKREDFVECIYNIVNKGN
                     YCYLEEFIIEEAENMKIGAYEYTGYLKCVNSVESYFEMSKDLLNLEVADELLYSERKI
                     FTKEKNESPTIYTDSAKVENSFIASGCLIEGTVKDSIIFRKVNVEKGTVIENSIVMQN
                     CVIKSNAKLYNAILDKNTSVSNGKELRGDEKYPLVVEKNTNI"
     gene            2705..4147
                     /gene="glgA"
                     /locus_tag="PC414_003301"
     CDS             2705..4147
                     /gene="glgA"
                     /locus_tag="PC414_003301"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011053260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HDF2465673.1"
                     /translation="MKVFYVTAECWPFAKTGGLGDVSYALPKELKKEGVDVRVIMPKY
                     STIPSYLKDQLKEIAVFSVRVGWRNQYCGLLEMELDGVKFYFIDNEFYFRREDERKSI
                     YGYGDDAERYTFFTDAVLEAISRIDFYPDVIHINDWHTGMLPLILKERYATLEGYKNI
                     KTMYTIHNLQYQGVFDKHVLYDILDLPQKYFDNGDIEYYGSINFMKAGINFADKIITV
                     SPTYANEIQTSFYGEQLDGLLRKESGKLKGILNGIDYDLNDPAKDKDIFVHYDVDSID
                     KKVENKLRLQDILGLKKDSSIPLIGIVSRLVSQKGFDLIAYMMPELMREDLQIVVLGT
                     GEHQYQSMFNYYDSNFSDKVSARITFNASLAQQIYAASDMFLMPSLFEPCGIGQMLAM
                     RYGSLPIVRETGGLRDTVTPYNKFTGEGNGFSFKNYNAHEMFFCLKNAIKVFKDKEKW
                     IKLVENAMKTDNSWKKSAKEYIETYRDICD"
     gene            4161..6581
                     /locus_tag="PC414_003302"
     CDS             4161..6581
                     /locus_tag="PC414_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HDF2465674.1"
                     /translation="MVTISKKKFKQKFEVKMYSLYAQSIKEATDEQLLNVLCSLLKDE
                     IAKKWVATKLDKKKEVYYFSLEFLIGRQLKSNLLNLNIEEEVREGLSELGINLDDLIE
                     AEVDPALGNGGLGRLAACFLDSMASLNISGQGYGIRYKYGLFEQKFVNGYQVEVPDNW
                     LTEGRYAWETVRPNEATMVKFGGEVELIKEGSHLKVIHKNYLPVMAMPYDIPIIGYQN
                     QCINTLRLFKSEIPKRDFGELTSNALNYSGSYEEALKHKYYTEEISQVLYPDDSNYAG
                     KLLRLKQEYFFVSAGIQDIIRKYKKNKLNINNLFDKVAIHINDTHPTLCIPELMRVLL
                     DEENLSWDEAWEITKKTVSYTNHTIMSEAMEKWPVSMMKELLPRIYMIIEEINRRYVE
                     ELNNKGYDQDRIKRMSIIDCDNINMANLCIVTSHSVNGVAKLHTHILETEVLKDFYQD
                     EPNKFNNKTNGIAHRRWLISSNPQLSNLITDLIGDSWKKDTLQLKNIEKFKNDSSVLQ
                     RLDDIKYNNKANLAKFIKDKYDLNVDPSSIFDVQVKRLHAYKRQLLNIFNVLHMYHEL
                     LDNPNLNLDPRTFIFGAKAAPGYYLAKCIIKFINSVASTINNDVRVKDKLKVVFLENY
                     GVSLAEIIIPAANVSEQISTTTKEASGTSNMKFMMNGAITLATLDGANVEICEQVGKE
                     NMFLFGLSAEQVLNYNKYGGYSSLDLYHSNMDIKRVVDDLINGFIPNLGEEGRSIYNS
                     LTTYNDEYFVLRDFENYGQAQADINRLYRDKEKWNKMSLVNIANSGFFSSDRTISEYA
                     KDIWFKRV"
     gene            6586..8451
                     /locus_tag="PC414_003303"
     CDS             6586..8451
                     /locus_tag="PC414_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HDF2465675.1"
                     /translation="MQSVIEYNSWDKNFKAPFGALKFDEELTICVKVNEGYNIKSISL
                     EINREEEMRTITLNEELDNDKLGKCFCGKIEKFDGTGVYFYYFKVDVEMDGQIKTLFY
                     GKNRDNGYSCEYNYSDINKYQITVYKDFKVPTWYKEGILYHIFVDRFNNGNRSGKVDN
                     PKKNSFIYGNWEDTPMYIKDSQGDVIRWDFHGGNLRGIINKLGYLKKLGVSILYLSPI
                     FEASSNHKYDTGDYKKIDPMFGDEDTFKELIDKAKEKGISIVLDGVFSHTGADSKYFN
                     MYGNYNSLGAYQSKESPYYSWYMFEEFPQKYKSWWDVKTLPNINELEHSYMDYIIYDN
                     DSVINKWVNMGIKGWRLDVADELPTKFIRELKKELKKADDDSILIGEVWEDASNKISY
                     GQRRSYLLGEELDSVMGYPFRNNMFSFLKGEINSYELCNRYMQIKENYPKESFRSNLN
                     LIGTHDVTRAKTELNDDVDLVKLAVAIQMTFEGVPYIYYGDEAGLCGGVDPDNRRTYP
                     WKNEDEDMLNFYRDVIRIRNKNKLLSSGNTEFIYTNNDSVFSFIRVNENNDRMLILVN
                     RSENVESISLCIESSFIEEIPIKYSLKNANSTIQIENNELKVDLDSKSFRIFRVN"
     gene            8696..9349
                     /locus_tag="PC414_003304"
     CDS             8696..9349
                     /locus_tag="PC414_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2465676.1"
                     /translation="MKVDPLTTQVYDYISKKIQNGEYEANQRITESEICKSIGVSRTP
                     AREALTRLAGENLLEKIPNKGFVVKEFQEKEKLDTYSVIGVLDALAGSSALQNLTESD
                     LVKMEELTEMMAVSIKYKNYNSYLKLSNEFHDIYITKSDNQVLVNLLNSLRYNFMSKS
                     YTSDNEDELYKMLTYSNNQHIEVVKSMRENDLENVERILREHWKTIPMEEMKKEKLL"
     gene            9445..10044
                     /locus_tag="PC414_003305"
     CDS             9445..10044
                     /locus_tag="PC414_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HDF2465677.1"
                     /translation="MYPNKEIAIEELEVAGKMNPGPWIKHSYNVANVAKLIAEQCENL
                     NSEKAFVCGLLHDIGRRTGISSVRHIIDGYDYVISKGWDEVARVCLTHSFPVKDIEAD
                     IGNKDISKEQYDFINHYLNNLEYNDYDKLIILCDALADANGFCILEKRFVDTTRRYGI
                     YPFSVERWNKTYEYKEYFEKIINKSIYTLLPNIEKSIYY"
BASE COUNT         4117 a         1002 c         1867 g         3271 t
ORIGIN      
        1 acttgatttt ccttggataa ttatttaaca aatattagat tatattttgc tattacgaat
       61 ttaacaaatt aaaaaataag catgttcata tgacacaaag gatttataaa aaatgttttt
      121 atatttataa aataaaattt gtattatttt ttaaccgata attgaatatt aataaatact
      181 atagtgctta tggaaaataa tgtatacgtc ggtaagtcca ttatggggta tacattatat
      241 caattgtata gttgatatta ttttattttt attaaggtat tatttaagca acatttatac
      301 acgacattta ttgactgata aagtttataa aagtaaaaat gcaattataa atattaatta
      361 ctagatgagg ggagtgtcat taaatgaaaa aagagatgtt agctatgatt ttggcaggag
      421 ggcaaggttc aagattaggt gtttttacaa aaagaattgc caaaccagct gtatcatttg
      481 gaggtaagta tagaattata gattttgtgt taagcaattg ttcaaactct ggtattgata
      541 ctgtaggtgt tttaactcaa tatagaccgt tgatattaaa ttcacatata ggcatgggaa
      601 gtcattggga tttggacaga ataaatggtg gtgtctatgt tttacaacca tttatgaatg
      661 aaaaagaagg aaattggtat aatggtacag cacatgcgat atatcaaaat atggactttg
      721 tagataccta taatccagaa tatgtattga tattatctgg tgaccatata tacaagatgg
      781 attatagcaa aatgttaaaa tttcataagg aaaaaggctc taaagctaca atagcagtga
      841 ttgaagttcc ttgggatgaa gcttcaagat ttgggataat gaatacaaat gaagattcaa
      901 gtatctatga atttgaagaa aaaccaagtg aacctaagag caatttggca tctatgggag
      961 tatacatatt tgattggaaa atgttgagaa attattttaa ggaagcagaa aaaaatccag
     1021 aaataaatta tgatgatttt ggaaaaaatt taatacctaa aatgttagaa gataatgttg
     1081 gaatgtatgc ttatccattt aaaggatact ggagagatgt aggaactata caaagtctat
     1141 gggatgctaa tatggatata ataaaatctc cagaaacact tgatttagca gaccctaagt
     1201 ggaagatata tacaaatact atggcaatgc ctccacaata tataggtaaa aatgcaaatg
     1261 ttcatagatc aatgatagct gatggttgta gaattttagg tgaagtaggt aactctgtat
     1321 tatcacatgg agttgttgtt ggtaaaggaa gtaaagtaat tgattcagta ataatgccaa
     1381 atgttgtaat aggtgaaaat gtaactatag aaaaagctat gataggtgag tgtgcaacta
     1441 taaatgataa tgttcagata aagaatgtaa ataatgaaat aaatgtagtt tctgagtatg
     1501 aaaatatcga acctagatgc gtattaatag aaggaggtct ttaagatgag aaatgaatgt
     1561 ttagggataa taaatttaaa caaaaaagga gacccagcaa taaacaaact taactatgga
     1621 agaccaatag catctacacc aatagctgga agatatagaa taatagactt tgctttatct
     1681 aatatgataa attcaggaat cactaaagtt ggaatattcg caaaagaaaa atacagatca
     1741 ttaacagacc atataggtag tggtaaagat tgggatttaa gtagaaaaaa aggtggccta
     1801 tcaattttta gtccagaaaa cactaagtat agaaatactt actcacatag agaaggtgat
     1861 atatatacta tcttagctaa tctagattat atagaaaaga gtgaagaaga atatatttta
     1921 atagctccaa gctatatgct atgcaattta aattattctc aagcattgga ataccataaa
     1981 aaatctcata atgatataac tataatatat aaaaatgtaa ataatgcaaa taaagatttt
     2041 gctggtaact taactctaaa tttagatagt aacaataggg taataaatgt aggaaataat
     2101 ttaggtaagt tccctagagc aaacatatgt atggagacat atattatgaa gcgtgaagat
     2161 tttgttgaat gtatatataa cattgtaaat aaaggcaatt attgttattt agaagaattt
     2221 attattgaag aagcagaaaa catgaaaata ggagcctatg aatatacagg atatctaaaa
     2281 tgtgtaaatt ctgttgaatc atattttgaa atgagcaaag atttattaaa tttagaagtt
     2341 gcagatgaat tattatattc tgaaagaaag atatttacaa aagaaaagaa tgaatcacct
     2401 acaatttata cagatagtgc taaagtagaa aatagcttta tagcatctgg ttgtttaata
     2461 gaaggtactg taaaagatag tatcatattt agaaaggtaa atgttgaaaa aggaacagtt
     2521 atagagaact caatagtaat gcaaaattgt gtaataaagt caaatgcaaa actatataat
     2581 gcaatattag ataaaaatac aagtgtatca aatgggaaag aattaagagg agatgaaaaa
     2641 tatcctcttg tagtggaaaa gaatacaaat atataaataa attttttaga agggggaatg
     2701 actaatgaag gttttttatg taacagcaga gtgttggccc tttgcaaaaa ctggaggatt
     2761 aggagatgtt tcttatgcat tgcctaaaga attaaaaaaa gaaggtgtag atgtaagggt
     2821 aataatgccc aaatattcaa ctataccaag ttatttaaaa gatcaactaa aagaaatagc
     2881 tgtgtttagt gtacgtgttg gatggagaaa tcaatactgt ggtcttttag aaatggaatt
     2941 ggatggtgta aaattttact ttatagataa tgaattttat tttagaagag aagatgaaag
     3001 aaagagtatc tatggatatg gagatgatgc agaaagatat actttcttta cagatgcagt
     3061 tttagaagct ataagtagaa ttgattttta tccagatgta atacatataa atgattggca
     3121 tacagggatg ttaccattaa ttctaaaaga aagatatgca acccttgagg ggtataaaaa
     3181 tataaaaact atgtatacta tacataatct ccaatatcaa ggtgtatttg ataagcatgt
     3241 tttatatgat atattggact taccacaaaa atactttgat aatggagata tagagtatta
     3301 tggctcaata aattttatga aagctggtat aaattttgca gataaaatta taactgttag
     3361 tcctacttat gcaaatgaaa tacaaacatc tttttatggc gaacaattag atggattact
     3421 tagaaaagag tcaggaaaat taaaaggtat attaaatggt atagattatg atttaaatga
     3481 ccctgcaaaa gataaagata tatttgttca ttatgatgta gactcaattg acaaaaaagt
     3541 agaaaataaa ttgagattac aagatatatt gggactaaaa aaagattcat ctattccgtt
     3601 gataggtata gtttcgagat tagtttctca aaaaggattt gatttaatag catacatgat
     3661 gccagaactt atgagagaag atttacagat tgtcgtttta ggaactggag aacatcaata
     3721 tcaatcaatg ttcaactatt acgattctaa tttttcagat aaagtatcag ctagaattac
     3781 ttttaatgca tcattggctc aacagatata tgctgcaagt gatatgttct taatgccatc
     3841 attatttgag ccttgtggta taggtcaaat gttagctatg agatatggtt ctttaccaat
     3901 agttagagag actggagggc ttagagatac agtaacacct tataataaat ttactggaga
     3961 aggcaatgga tttagtttta aaaattacaa tgctcatgag atgtttttct gtttgaaaaa
     4021 tgcaatcaaa gtatttaagg acaaagaaaa atggattaaa ttagttgaaa atgcaatgaa
     4081 aacagataat agttggaaaa aatcagctaa ggagtatata gaaacatata gagacatatg
     4141 tgattaaagg agtggttttt ttggtaacaa taagcaaaaa gaaattcaag caaaaatttg
     4201 aagtaaaaat gtatagctta tatgcacaat ctatcaagga agctactgat gaacaattgt
     4261 taaatgtatt atgtagtcta ttgaaagatg agatagcaaa aaaatgggtt gctacaaagt
     4321 tagataagaa aaaagaagtt tattatttta gtttagaatt tttaataggg agacagttaa
     4381 aatctaattt attaaattta aatattgaag aagaagttag agaaggttta tctgaattag
     4441 gaatcaactt ggatgattta attgaagctg aagtagaccc agccttagga aatggtggac
     4501 ttggtagatt agcagcttgc tttttagatt ctatggcatc tttgaacata agtggtcaag
     4561 gatatggaat aaggtataaa tatggtctgt ttgaacaaaa atttgtaaat ggttatcagg
     4621 tagaagtgcc tgataactgg cttacagaag gacgatatgc atgggaaact gtaagaccaa
     4681 atgaagcaac aatggtgaag tttggtggtg aggtagaatt aatcaaggaa ggtagtcatt
     4741 taaaagtaat tcataaaaac taccttcctg taatggcaat gccttatgat ataccaataa
     4801 tcggatacca aaaccaatgt ataaatactt taagattgtt taagagtgaa ataccaaaga
     4861 gagattttgg tgaacttacc tcaaatgctc taaattattc tggtagctat gaagaggctt
     4921 taaaacataa gtattatact gaggaaatat cacaggtatt atatcctgat gattcaaact
     4981 atgcaggtaa gttgttgagg ttgaaacaag aatacttttt tgtaagtgca gggatacaag
     5041 atataataag aaaatataaa aagaacaaat taaatattaa taatttattc gataaggtag
     5101 caattcatat aaatgataca catcctacct tatgcatacc agaacttatg agagttttgc
     5161 ttgatgaaga aaatttatct tgggatgaag catgggaaat aactaaaaaa acagtgtcct
     5221 atactaatca tactataatg tcagaagcaa tggaaaaatg gcctgtaagt atgatgaaag
     5281 aactattacc aagaatttat atgataatag aagaaataaa tagaagatat gtagaggaat
     5341 taaataataa aggatatgac caagatagaa taaaaagaat gagtataata gactgtgaca
     5401 atatcaatat ggcaaactta tgtattgtca caagtcatag tgtaaatgga gttgctaagc
     5461 ttcatactca tattcttgaa acagaggtat taaaagattt ctatcaagat gaaccaaata
     5521 aatttaataa taagactaat ggaattgcac ataggcgatg gcttataagt tcaaatcctc
     5581 aacttagcaa tttaattaca gatttaattg gtgattcatg gaagaaagat acattacaat
     5641 taaaaaatat tgaaaaattt aaaaatgatt cctctgtttt acaaagactt gatgatataa
     5701 aatacaataa taaagcaaat ctagcaaagt ttataaaaga taaatacgat ttaaatgtag
     5761 acccaagttc gatatttgat gttcaagtta agagattgca tgcgtataaa agacagttac
     5821 tgaatatatt taatgtactt catatgtatc atgaattact tgataatcct aatttgaatc
     5881 ttgacccaag aacttttata tttggcgcaa aagcagcacc aggatattat ttggctaaat
     5941 gtataattaa atttataaat tctgttgcaa gtactataaa taatgatgtt agagtaaagg
     6001 acaagttaaa agtagtattt ttagagaatt atggagtatc attagcagaa ataataatac
     6061 cagcagcaaa tgttagtgag caaatttcaa ctacaactaa ggaagcatct ggaacaagta
     6121 atatgaagtt tatgatgaat ggtgctataa ctttggctac acttgacggt gcaaatgtag
     6181 agatatgtga acaggttggt aaagaaaata tgtttttatt tggacttagt gcagaacaag
     6241 tactaaatta taataaatat ggtggatatt cttcacttga cctatatcat tctaatatgg
     6301 atataaaaag ggtagtagat gatttaataa atggatttat tcctaatctt ggcgaagaag
     6361 gaagaagtat ttataattca ctcactactt acaatgatga gtattttgta ttgagagatt
     6421 ttgaaaatta tggtcaagct caagcagata taaacaggtt atatagagat aaagaaaaat
     6481 ggaataaaat gtctttagtt aatatagcta attctggatt tttttcttct gatagaacaa
     6541 tttctgagta tgcaaaagac atttggttta aaagggtgtg attaaatgca aagtgtaata
     6601 gaatataact cttgggataa aaattttaag gctccgtttg gagctttaaa atttgatgag
     6661 gaattgacca tttgtgttaa agtaaatgag ggatacaata tcaagtctat ttccctagaa
     6721 ataaatagag aagaagaaat gagaacaata actttaaatg aagaattaga taatgataaa
     6781 cttggaaagt gcttttgtgg taagattgaa aagtttgatg gtacaggtgt ttatttttat
     6841 tactttaaag ttgatgttga aatggatggt cagataaaga ctttatttta tggaaaaaac
     6901 agagataatg gatattcttg tgaatataat tatagtgata ttaataaata tcaaatcact
     6961 gtttacaaag attttaaagt accaacatgg tacaaagaag gaatacttta tcatatattt
     7021 gtagatagat tcaataatgg aaatcgtagt ggtaaggttg ataatcctaa aaaaaatagt
     7081 ttcatatatg gaaattggga agatacacct atgtacataa aggatagtca aggggatgtt
     7141 ataagatggg atttccatgg tggaaattta agagggataa tcaataaact tggatattta
     7201 aaaaaattag gtgtaagtat actatatcta agccctatat ttgaagcctc tagtaatcat
     7261 aagtatgata caggtgacta taaaaaaatt gacccaatgt ttggggatga agatacattt
     7321 aaagaactta ttgataaagc aaaagaaaaa ggtatatcaa tagtattaga tggagttttt
     7381 agtcatactg gagcagatag taaatatttt aatatgtatg gaaactataa tagtttaggg
     7441 gcatatcaat ctaaagaatc tccatattat tcatggtata tgtttgaaga atttcctcaa
     7501 aaatataaaa gttggtggga tgttaagaca ttgccaaata taaatgaatt agaacatagc
     7561 tatatggatt atataatata tgacaatgat agcgttataa ataaatgggt aaatatgggt
     7621 attaagggct ggagactaga tgttgcagac gaattaccaa ctaagtttat aagagagtta
     7681 aaaaaggaat taaaaaaggc agatgatgac tcaattctta taggtgaagt atgggaagat
     7741 gcatcaaata agattagcta tggtcagaga agaagttatt tattaggtga agaattagat
     7801 tctgttatgg gttatccttt tagaaataac atgttttctt tcttaaaagg agaaattaat
     7861 tcatatgaac tttgtaatag atatatgcag attaaagaaa attatccaaa agaatcattt
     7921 aggtctaatt taaatttaat tggaacacat gatgttacaa gagctaaaac ggaattaaat
     7981 gatgatgtag atttagtgaa gcttgctgta gctatacaga tgacatttga aggagtacca
     8041 tatatatatt atggagatga agcaggtctt tgtggaggtg ttgacccaga taatagaaga
     8101 acttatccat ggaaaaatga agacgaagat atgcttaatt tttatagaga tgttataaga
     8161 attagaaata agaataaact tttaagttct ggaaatacag aatttatata tactaataat
     8221 gattctgtat tttcttttat tagagttaat gaaaataatg atagaatgtt aattttagta
     8281 aatagaagtg aaaatgtgga aagtatatca ttatgtatag agagtagttt tattgaggaa
     8341 atacctataa agtatagtct taaaaatgca aatagtacta tacaaattga aaataatgag
     8401 ttgaaagtag atttagactc taaatcattt agaattttta gagtaaatta aatatctttt
     8461 tttagaaaaa taaatagaaa ataaggtgaa tgtggaaaga gttttgtttt atcaaaactt
     8521 tttctacttt ttttgtataa attatcataa gaattttagt atttcaaaat attacaaaga
     8581 agtataaaaa atatcataat tgtgatatta tataaattag taagataaaa ctgaactctc
     8641 taccaaagtt gtagggagat ttaactttta taatcttaat atggaggaac gtcttatgaa
     8701 ggtagatcca cttacaactc aagtatatga ctatatatca aagaaaatcc aaaatggaga
     8761 atatgaagct aatcagagaa ttactgaatc tgagatttgt aaatctatcg gagttagtag
     8821 aacaccagcg agagaagcat taactaggtt agcaggtgaa aatttactag aaaaaatacc
     8881 taataaggga tttgtagtaa aagaatttca ggaaaaggag aaattagaca cctattccgt
     8941 aattggagta ttggatgcgt tagccggttc ttcagcatta caaaatttaa ctgaatcaga
     9001 cctagtaaaa atggaagaat taacagaaat gatggcggtc tctataaagt ataaaaatta
     9061 caatagctat ttaaaattaa gtaatgaatt tcacgatata tacataacta aatcagataa
     9121 tcaggtgtta gtaaatctat tgaactcttt aagatataat tttatgtcaa agtcatatac
     9181 tagtgataat gaagatgagt tatataaaat gttgacatac agcaataatc aacatattga
     9241 agtagtaaaa tctatgaggg agaatgattt agaaaatgtt gaaagaattt tgagagaaca
     9301 ttggaaaacg atacctatgg aagagatgaa aaaagagaag cttttataaa gtttctcttt
     9361 attttttata ccattttatc actatttttt tgaattatta agtagaaaat aataattaat
     9421 ttaaacttga ggaggaaaac taatatgtat ccaaataaag aaattgcaat agaagagtta
     9481 gaagtggctg gcaaaatgaa tcccggtcca tggataaaac attcttacaa tgttgctaat
     9541 gtagctaaat tgattgcaga gcaatgtgaa aatttgaata gtgaaaaggc ctttgtatgt
     9601 ggattattac atgacattgg aagaagaact ggtatttctt ctgtaaggca tattattgat
     9661 ggatatgatt atgttatttc taagggatgg gatgaagtgg cgagggtatg tttgacacat
     9721 tcttttcctg ttaaggatat agaagctgat attggaaata aagatattag taaagaacag
     9781 tatgatttta tcaatcacta tttaaacaac cttgaatata acgattatga taagttgatt
     9841 attctttgtg atgcattagc agatgcaaat gggttttgta ttttagaaaa aagatttgtt
     9901 gatacaacac gaagatatgg aatatatcca ttttcagttg aacgatggaa taagacttat
     9961 gagtataaag aatatttcga gaaaataatc aacaagtcta tatatacatt gttacccaat
    10021 attgaaaaat ctatatatta ttaaatccta agttaataat ttaatatcca gtatcaccag
    10081 cagttggtca ttatgattga ctgcttttat tatgcttata ttaaaaaaat acactatggt
    10141 ttgtgtatat caaagttata tggaatgaaa agagaattta ttttatcaat taaaaaattt
    10201 aataaatttt atattaaaat taaaatacaa aaataggaaa attatttagt ccaacta
//
LOCUS       DAMJVK010000123        10124 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.128, whole genome shotgun sequence.
ACCESSION   DAMJVK010000123 DAMJVK010000000
VERSION     DAMJVK010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10124
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.128"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            210..1091
                     /locus_tag="PC414_003306"
     CDS             210..1091
                     /locus_tag="PC414_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2465678.1"
                     /translation="MPAYLFDSNSIANIYRVFPISFSFIVTGLYFSYKNNKNNKINFE
                     AVLEDKKNLIFLLSLMFGMYLYLCLSLSIYYTVGNVLILKLYGIKSAIIVLVGNYIAE
                     RYSFRITQIKGYDIKNQIEYVESVKTEYEINKLNDIVYTDLLTGFYNRPFVNQKLSEW
                     TSNHYKFTLCFTDLNSLKPVNDKFGHQFGDKYISTTAQIIKKLINVENSLLFRYGGDE
                     FLFLLQNTSCVEAEKIMININYELSKLSNTDEYPYALSISYGLVEWDGISDIETLISK
                     ADSLMYENKLKNKSTLN"
     gene            1076..1449
                     /locus_tag="PC414_003307"
                     /pseudo
     CDS             1076..1449
                     /locus_tag="PC414_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418025.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(1709..4276)
                     /locus_tag="PC414_003308"
     CDS             complement(1709..4276)
                     /locus_tag="PC414_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HDF2465679.1"
                     /translation="MIYYVLPLEHKQATIEIVGGKGMSLSKLLTAGIPVPDGFHVTTA
                     SYQIFVETNHIQSRINKLLDGIDSNNTSQLEDVSKKIGELFHNGEMPQEVSDAIKMAY
                     AGLGNISVAVRSSATAEDLPDASFAGQQETYLNIQGEDKVIDSVKRCWASLWTARAIA
                     YRVKNNIKHEIVALAVVVQKLAFSDSSGIMFTLNPINGRRSEMIINAAWGLGEAVVSS
                     LVTPDTIVVDKDSERIISYEVANKEIMTVRTSEGTEETMVPERLRKKYALTRNQVMQL
                     IQLGKKIEKYYQMPMDVEWALEKDKLYIVQARPITVLPPEWVLPEQDVVYTKGSLAEH
                     LPNPVTPLFATLGLEIVNRASELLWIDMFGKSAKKLLPKNGAYTVINGYVYLSAKSKP
                     LLIAIKSLSPRSLRRTLMNSVARWETARKGYEDAIKQWEEKPLHTMNAHQIMEGIQTI
                     FYAACIYFTRIQLTLPAASISETLFTKLFQGAVRRAGITDTSVFLLGFDTIVLQSEKE
                     LWGISEWVKQNNSINLYLQNSPAIKIAEDFMSSVVPVGVSQEVWIEWKNRINKYFKEF
                     GRTAYEFDFAHSTPQETLTPTFESVKTFVEGKGESPFLRQTTFEKRRKHAEEEILQHI
                     GGLRKKLFFKLLHWAQETSPMRENAIYLMGMGHPLIRCMFQEISERFIRGGAISHLDD
                     IYWLTKSELEVLIEQLDKNKSLSDMNGIIPVRKAELKKYMGYMSPSKLPEKNKKTISQ
                     AQQKQKDGKIVLTGIGTSTGVVTAPACVLNSPADFESFQPGSVLVAVTTTPAWTPLFS
                     SASAIVTDIGGPLSHSSIVAREYGIPAVMATHIATRTIKSGQMITVDGLAGTVTFDE"
     gene            complement(4279..4884)
                     /locus_tag="PC414_003309"
     CDS             complement(4279..4884)
                     /locus_tag="PC414_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2465680.1"
                     /translation="MPKGIVLTPEQQAERREKIVAVALSLMAENGFQKTSMREIAVLA
                     NMGKSSLYDFFKTKDEIVVYAVEKEIEKTIQQVHRIIDGEPSPQQCLRKIMLNHLRFP
                     EQYRTVLMWLNTESDYLEEEYRKRLKDARYAYQDIIKSVIDNGVTTGIFRKTDTALVT
                     RLLINSMLSISYTSRPLDSSEKMLDETMNIFLNGIMNDGGE"
     gene            complement(5258..6718)
                     /locus_tag="PC414_003310"
     CDS             complement(5258..6718)
                     /locus_tag="PC414_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HDF2465681.1"
                     /translation="MFNELKTKENVYKNLINGKWVESNSRKPIEIYSPIDNSLVGKVQ
                     SMTKHEVDEVIKNTKESIKVWAEMPVYKRGNIFHKAADLLLENIDEIANILVLEIAKD
                     IKSARAEVERTADFLRYTADVGKNMEGEAISGDNFPGGTRNKMSYVSRVPLGTVLAIS
                     PFNYPVNLSMSKIAPALIGGNAVVLKPATQGAISALHVVEIMRKAGIPDGVLNTITGR
                     GSEIGDYVVTHKGINFINFTGSTEVGQHISKISGMVPLLLELGGKDAAIVLEDADLDF
                     AAKNIVSGAYSYSGQRCTAVKRILVQESVADKLVGKIKPLVEKLTIGNPMDEVVITPL
                     IDNKATDFVQGLVDDALHKGAKLITGNVRKNNLFYPTLLDNVNVDMKIAWEEPFGPVL
                     PIIRVKDINQAIEIANQSEYGLQSSVFTSDIDKAFYIADKLEVGTVQINNKTERGPDH
                     FPFLGVKASGMGTQGVKYSIEAMTRPKAVVVNVREL"
     gene            7088..7666
                     /locus_tag="PC414_003311"
     CDS             7088..7666
                     /locus_tag="PC414_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2465682.1"
                     /translation="MAKSFTEIEKDNIRKRLISECEINWSKYGYKKTNIDELCSKSGI
                     AKGSFYTFFDSKEKLFFEVLNGIQNRLISSVDEILSNMPPKEGFAKSLKMLYRLYDKN
                     PFLYSPNNPDFISLINKLPQDMFLQLESNNIQSFYDLIERHNLKLKIEKEKVFGVCNA
                     LLSSLLVKENIEYNYIEVFDFMVDNLVEHILE"
     gene            7957..9207
                     /locus_tag="PC414_003312"
     CDS             7957..9207
                     /locus_tag="PC414_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2465683.1"
                     /translation="MVKSSNTFNKNFNLMILGQIISLFGASILKFALSLYILDITGKA
                     EIFATILAVSSIPIIVFSPIGGAIADIFNRRNLMVIFDFSSSLTVLILAVFLFNNNGS
                     ILLVGIIMTILSVISTMYQPTVQSSTPLLVDNEHLMNANGIVAGVASLTNIAGPVLGG
                     VLYGVIGINAIIVISCVSFFLSAIMEIFIQIPFIKQEQNSNIIKTIFTDIKDGIVHIC
                     KENRFILKILFLAIAINLFLSSMLIVGIPYIVKITMGASNFLYGLAEGAISFSSLVAA
                     VSIGIFSKHLKITNLHISFIICAVMFIPMAVSVYPFILNTGFPVPFLIFIVSSMLIFF
                     TTTLISIYVITVTQLKTPNELLGKVMSILFAGTAIAMPLGQVMYGKLFDLLSDRVYII
                     ILGVGFITLLIAFVSKITLRNENL"
     gene            9368..9937
                     /locus_tag="PC414_003313"
     CDS             9368..9937
                     /locus_tag="PC414_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDF2465684.1"
                     /translation="MSYMPTLEQAWEILRKYNKEEFHIRHAQIVSGVMRYYAKEYDPE
                     RVEFWEIVGLLHDLDFEMYPDEHCVKQRELMTELDLDKSIIDSTISHGYGLTGSDVKP
                     EQFMEKVLFAVDELTGLIGAAAIMRPSKSVSDLELKSVKKKFKDKSFAAGCSRDVIRS
                     GAEMLGWELDKLIQTTIEAMRSLIPEMAI"
BASE COUNT         3390 a         1735 c         1478 g         3521 t
ORIGIN      
        1 tatttaatta tttaattttt attatttgta cttatttaaa gtttcatcta attataaatt
       61 ggacaatttt tatgttgatt ttattcatac aaattaaagt aacttataaa atctccacaa
      121 taaaaaacat tttttattca ctaaactgct ccatcattgg tttagcaata aatatatttt
      181 ttaggtcttt aatgtcaata gtgttaaata tgccagcata tttatttgat agtaattcta
      241 ttgcaaatat ttacagagta tttcctatat catttagctt tatagttaca ggactatatt
      301 tcagttataa aaataataaa aataataaga ttaactttga ggcagtttta gaggacaaaa
      361 aaaacttaat tttcttattg agccttatgt ttggaatgta cttgtatcta tgtttgagtt
      421 tgtcaatata ctatactgtt ggaaatgtat tgattttaaa attatatgga atcaagtctg
      481 ccataatagt tttggtaggt aactatattg cagaaagata tagctttaga attactcaaa
      541 taaaaggtta tgatattaaa aatcaaatag aatatgtaga aagtgtaaag acagaatatg
      601 agattaataa attgaatgat attgtatata cagatttact gacaggtttc tacaatagac
      661 cttttgtgaa tcaaaaacta tcagaatgga catcaaatca ctacaaattt accttatgtt
      721 ttactgattt aaatagctta aaacctgtaa atgataaatt tggacaccaa tttggtgata
      781 aatacatatc cactactgct caaataataa aaaaacttat aaatgtagaa aattccttac
      841 tatttagata tggtggagat gaatttttat tccttttaca gaatacaagt tgtgtagaag
      901 cagaaaagat aatgattaat ataaattatg aattatcaaa attaagtaat actgacgagt
      961 atccttatgc tctttccata agctatggat tagttgagtg ggatggaata tccgatattg
     1021 aaactttaat atcaaaggca gattctttga tgtatgaaaa taagcttaaa aataaatcta
     1081 ctttaaacta gctagttaag atattctaca aaatatatgt tataagagag tactaagaat
     1141 atcaaaatat atagattttg caaatattga atacagttta ctaatgaatg cactggatgt
     1201 aagtgttagt aaacacctat tagatgaaca ctttactgtt gtttgggcta atgaccgtta
     1261 ttatgaaatg ttcagctata cctagctaaa aagttaaaaa tgaccactgt agccgaggga
     1321 atagagacca gttttcaaaa agatttcttg caagaaataa actgtgatat gttacaagga
     1381 tacgtgttct ctagaccttt accaataaaa gactttgaaa aacttatgtt tcaaaactcc
     1441 tccaattaac aatataaaat tgcttattat aagtgatatt attgtatcaa caaataaagg
     1501 aattgtctca aaattgattt tttctaatca aatttttatt ttgagacatt ctcatttttt
     1561 tgcataagaa aatacccacc aagctacggt ttttcttacc gctgttcggt gggtattagt
     1621 agtattatga aatctgaaaa ctttaagtat tactacttcc gctaacgcaa tcacaaaggt
     1681 cgaaagcttc aaagcgaaaa taggcttttt actcatcaaa cgtcaccgta cctgccaacc
     1741 cgtccaccgt tatcatttgt ccgctcttaa tagttcgtgt ggcaatgtgt gtagccataa
     1801 cggctgggat gccatattca cgtgcgacaa tgcttgaatg gctaagagga ccaccaatgt
     1861 ccgttacaat cgcacttgca gatgaaaata atggtgtcca tgctggagtt gtggtaactg
     1921 caaccaaaac acttccaggt tgaaagcttt caaaatctgc tggactattc agtacacaag
     1981 ctggagccgt cacaacacca gtactggtac caatgcccgt caacacaatt ttcccatctt
     2041 tctgcttctg ttgtgcttgt gatatagttt ttttgttttt ctctggcaac ttggatggtg
     2101 acatataacc catgtatttt ttaagttcag cttttcgaac aggtattatc ccgttcatat
     2161 cagatagaga cttgttttta tccaactgct ctataagtac ctccaattcc gattttgtga
     2221 gccaataaat atcatctaaa tgtgaaatag ctcctcctcg tatgaatcgt tctgagattt
     2281 cctgaaacat acaacgaatc agaggatgtc ccattcccat caaataaata gcattttcac
     2341 gcataggaga ggtttcctgt gcccaatgga gcaacttaaa aaatagtttt ttacgcaatc
     2401 cacctatgtg ttgtaaaatc tcttcttctg catgttttct acgcttttca aacgtggtct
     2461 ggcgtaaaaa aggactttcc ccttttcctt ccacaaatgt ttttacggat tcaaaagttg
     2521 gtgtaagtgt ttcctgcggt gtggaatgcg caaaatcaaa ctcataagcg gtacgaccaa
     2581 actctttaaa gtacttattg attcgatttt tccattctat ccatacttcc tgtgaaactc
     2641 caacaggcac aactgaagac atgaaatctt ctgctatctt tattgcagga ctgttttgca
     2701 gataaaggtt gatgctgtta ttttgcttta cccattctga aataccccac aactcctttt
     2761 ccgattgcag aacaatcgta tcaaacccaa gcaggaaaac ggaagtatcc gttataccag
     2821 cacgtcgaac tgctccttga aataattttg taaataatgt ttcactgata gaagcagcag
     2881 gcaatgtaag ctgaatcctt gtgaaataaa tacatgcggc ataaaatatt gtctgaatac
     2941 cctccataat ttgatgggcg ttcatagtat gcaggggctt ttcttcccat tgtttaatag
     3001 catcctcata tccttttcgt gccgtttccc agcgtgcaac gctgttcata agtgtcctac
     3061 gtaaagagcg tggggaaagg gatttgatag caatcaaaag tggctttgat ttagcagaaa
     3121 gataaacata tccattaata accgtatatg ccccgttttt tggtaataat tttttcgcac
     3181 ttttaccaaa catatctatc cataaaagct ctgatgcacg gtttacgatt tcaagaccaa
     3241 gtgtggcgaa caaaggtgta acaggatttg gcaaatgttc tgccaaactt ccctttgtgt
     3301 atacaacatc ctgttctggc aatacccatt ctggtggcag aacagtaatt ggacgagcct
     3361 gaacaatgta caatttatct ttttccagtg cccactctac atccataggc atttgataat
     3421 acttctcaat tttctttcca agctgtatca gttgcattac ctgattacga gtaagagcat
     3481 atttttttct caacctttca ggaaccatgg tttcttctgt tccttctgaa gtacgaaccg
     3541 tcataatctc tttatttgcc acttcatacg atataattcg ttcggagtcc ttatccacta
     3601 cgattgtatc gggtgtaact aagctagaaa ccaccgcttc accaagcccc catgcagcgt
     3661 taataatcat ttcgctacgt ctaccattga ttgggtttaa cgtaaacata ataccagatg
     3721 aatcggaaaa tgcaagcttt tgtactacaa cagcaagtgc gacaatctcg tgctttatat
     3781 tattcttcac acgataagca atagcacgag ctgtccataa ggaagcccag catctcttta
     3841 cagagtcaat aactttatct tcaccttgta tgttaagata agtttcctgc tgacctgcaa
     3901 aagaagcgtc aggtaaatct tcagcagtcg cagacgaacg gacagccacc gatatgttcc
     3961 ctaatccagc ataagccatt ttaattgcgt ccgacacttc ctgtggcatt tctccattat
     4021 ggaaaagctc ccctattttc ttagatacat cttcaagttg gctggtatta ttggaatcaa
     4081 taccatctag cagtttatta attcgagact gaatatggtt cgtctcaaca aaaatctggt
     4141 atgaagcagt cgtaacatga aaaccgtctg gcacagggat gcctgctgtc aatagttttg
     4201 ataaagacat cccttttcca ccaacaattt caatggttgc ttgtttatgt tccagtggca
     4261 agacataata aatcataatc attcacctcc atcattcata attccgttca gaaatatatt
     4321 cattgtttca tccaacattt tttccgaact atccaatggt cgtgacgtgt agctgattga
     4381 taacatggag ttaattaaca aacgtgtcac aagagcggta tccgttttgc gaaagatgcc
     4441 tgtggtcaca ccattatcaa ttaccgattt tataatatct tgatatgcat aacgagcatc
     4501 tttaagtctc tttcgatact cttcttctaa ataatcagat tctgtattta accacatcag
     4561 tacagttcta tactgctctg gaaatctaag atgattaagc atgatttttc taaggcactg
     4621 ttgtggtgaa ggttcaccat caataatcct atgaacttgt tggatagttt tttctatttc
     4681 tttctcaact gcatatacaa caatctcgtc tttcgtttta aaaaaatcgt atagacttga
     4741 ctttcccatg ttggctaaaa ctgcaatttc tctcattgat gttttttgga atccattttc
     4801 agccatgaga ctcaaagcaa cagcaactat tttttctcgg cgttctgctt gttgttcggg
     4861 tgtaagcaca attccttttg gcatagtgcg atacctcctt ttagcgacca ctggtcgttt
     4921 cttttttatt tatcatacag caaccaacgg tcacttgtca aggttaattt ttttcattgt
     4981 atatggtact cgagataaaa atgttcattt tagaaattac tctatttaag tagaaatagg
     5041 cttcatcatt agaaagcaag actataaatt tgagatattt atcatgttca aatttcatca
     5101 atatcaaagt ttaaaatatt aataaaaaaa gaattactaa gaaataacat ataaagattc
     5161 aaaaaaggag ttgaacttaa aattcaatta caaaaaatca aatttaagac aactccttta
     5221 ttctttatct ctactattct ttatctctat ttatttatta caactcccta acatttacca
     5281 ctactgcctt aggtctagtc atagcctcaa ttgaatactt aacaccttga gttcccatac
     5341 cagatgcctt tacacctaaa aatgggaagt ggtctggacc tctttcagtc ttattattta
     5401 tttgaacagt accaacttca agtttatcag ctatataaaa tgccttatca atatcacttg
     5461 taaatactga tgattgaagc ccatactctg attgattagc aatttcaatt gcttgattta
     5521 tatctttaac tcttataatt ggaagtactg gaccaaatgg ttcttcccaa gcaattttca
     5581 tatcaacatt tacattatct aacaatgttg gatagaataa attatttttt ctaacattgc
     5641 ctgttattaa ctttgctcct ttatgtaatg catcatcaac caacccttga acaaaatcag
     5701 ttgctttatt atctataaga ggagtgataa ctacttcatc cattggattt ccaattgtca
     5761 acttctctac caatggcttt atttttccga ctaatttatc cgccacactc tcttgtacta
     5821 aaattctctt tactgccgta catctttgtc ctgaatatga atacgctcca gatactatat
     5881 tcttagctgc aaaatctaag tctgcatctt ctaatactat agccgcatct tttccaccaa
     5941 gctccaataa taatggtacc attccagata ttttagatat atgctgacca acttcagtac
     6001 taccagtaaa gttaataaaa ttaattcctt tatgcgttac aacataatcc cctatctcac
     6061 tacccctacc tgtaatagta ttaagtactc catctggtat cccagctttt ctcataattt
     6121 ctaccacatg aagtgcgcta atagcccctt gtgtagcagg ttttaatacg actgcatttc
     6181 caccaataag tgctggagca atctttgaca ttgataaatt tacaggataa ttaaaaggag
     6241 atatagctaa aacagtacct aatggaaccc ttgaaacata agacatctta tttcttgttc
     6301 caccagggaa attatctcca ctaatggctt ctccctccat attttttccc acatcagctg
     6361 tgtatcttaa aaaatctgct gttctctcaa cttctgccct tgcagatttt atgtctttgg
     6421 ctatttcaag taccagtata ttagctatct catctatatt ttctaacaat aaatcagcag
     6481 ccttatgaaa tatattgcct ctcttgtata caggcatttc agcccatact tttatactct
     6541 cttttgtgtt ttttataacc tcatctacct catgttttgt catagattgt actttcccaa
     6601 caagagaatt atctattggt gagtaaattt ctattggctt tctcgaatta gattctaccc
     6661 attttccatt tattaaattt ttatatacat tttcttttgt tttaagttca ttaaacatat
     6721 ttttattcct ctctctgata taactttgtg tatttaaatt atagccattt attcgttatg
     6781 taaacatttc tttttttcat aaacgtttac ctataaaaaa attttaagtt aacgtttttc
     6841 tacattcttc tattttaata acatactcgc tccaaatcta tataggtcac actttaatac
     6901 taatatatca atataccaat ataccaatat accaatatac caatatacca atataccaat
     6961 ataccaatat aaatattata aacataaatc acttgacaaa tttataagta gacaatatta
     7021 tatatctatg actcaaaata tttttaaagt catatatatc atatatagac agaaaggatt
     7081 gataaaaatg gctaaaagtt ttacagaaat agaaaaagac aatataagaa aaagactaat
     7141 ttcagaatgt gaaatcaact ggtctaaata tggatacaaa aaaactaata ttgatgaact
     7201 ttgttcaaaa tcaggaattg caaagggttc tttctataca ttttttgatt caaaagaaaa
     7261 gttatttttt gaagtattaa atggaatcca aaacagactt atatcatcag tagatgaaat
     7321 tttaagtaac atgcctccaa aagaaggctt tgcaaaatcc ttaaaaatgt tatatcgatt
     7381 atatgataaa aatccatttt tatattcacc aaataatcct gatttcatca gcctaataaa
     7441 taaactacct caagatatgt ttttacaact tgagtccaat aacatacaaa gtttttacga
     7501 cttaattgag agacacaatc tcaagttgaa gatagaaaaa gaaaaagttt ttggtgtttg
     7561 taatgctcta ctatcaagtc tgttggtaaa agaaaatatt gaatataact atattgaagt
     7621 gtttgacttt atggtggata atcttgtaga acacatactt gaataacaat ttaaaataat
     7681 ttttctaagt atttttttat actaaatagg attaatatga ttgaattaat gcaaaaaata
     7741 aacaatataa tttataaaat aaatgataaa ctaaatatca cacaaaactt atagacccta
     7801 gagcaaaccc attccactct actctaagcc atctataata gtttgtatat ttctaataat
     7861 attagatatt aaagtaagaa aaaaattcct tataataatt gctattatat caattattgt
     7921 aaatgtttta ataatcttag ataggtaggt acatatatgg taaaatcctc gaacactttt
     7981 aataaaaact tcaacttgat gatattaggt caaataatct cattgtttgg tgcatcaata
     8041 cttaaatttg ctttatcttt atacatactt gatataactg gtaaagcaga aatatttgct
     8101 actatactag cagtatcttc aatcccaatt atagtattct cccctattgg tggagctatt
     8161 gcagatatat tcaacagaag aaacttaatg gttatttttg atttttcaag tagtcttaca
     8221 gtactaatct tagcagtatt cttattcaac aataatggtt caatattact tgtaggaatt
     8281 attatgacca tattatcagt cataagcaca atgtatcaac caactgttca gtcaagtaca
     8341 ccccttttag ttgataatga gcatttaatg aatgcaaatg gtattgtagc tggagttgca
     8401 tctcttacaa atattgcagg acctgtatta ggaggtgtct tgtatggtgt gattggaatt
     8461 aatgcaatta tagttataag ttgtgtaagt ttctttctat ctgcaattat ggagatattt
     8521 atacagattc cttttatcaa acaagagcaa aacagcaaca ttataaaaac tatatttact
     8581 gatataaagg atggtattgt tcatatatgt aaagaaaata gatttatact aaaaatactt
     8641 tttttagcta tagcaataaa cttattttta agctctatgt taatagttgg tatcccctat
     8701 atagtaaaaa tcactatggg agctagtaac ttcttatatg gtctagcaga aggagcaatt
     8761 tcttttagtt cactggtagc tgctgttagt attggtattt tctcaaaaca tctaaaaatc
     8821 actaatttgc atatttcctt tattatatgt gcagttatgt tcataccaat ggcggtttca
     8881 gtatatccat ttatattaaa cacaggattt ccagttccat ttttaatctt tatagtatca
     8941 tctatgctaa tattttttac aacgactctt atctcaatat atgttataac agtcacacaa
     9001 ttaaaaactc caaatgaact cttaggaaaa gtaatgtcaa ttctttttgc aggaacagca
     9061 attgcaatgc cccttggtca ggttatgtat ggtaaacttt ttgatttatt atcagataga
     9121 gtgtacatca ttatactagg agttggattt attacattac ttatagcatt tgtatcaaag
     9181 ataacattga ggaacgaaaa tctttagata caaagaattt agatataaag atttaatttt
     9241 cactaatttc tgaatttaaa tgcaaaattg tgtatcagta tgttgacgtt ttagaaattg
     9301 attgatataa tcaatggaaa ataaacttta taaaaattca aaatattcag ctagaggagt
     9361 gataaaaatg agttacatgc caactttaga acaagcatgg gaaatattaa gaaaatacaa
     9421 taaggaagaa tttcatataa gacatgctca aattgtatct ggagttatga gatactatgc
     9481 aaaggagtac gaccctgaaa gagtagaatt ttgggaaata gttggacttt tacatgattt
     9541 agattttgaa atgtatcctg atgaacattg tgttaaacaa agagaattaa tgacagagct
     9601 tgatttagat aaatcaataa tcgattctac aataagtcat ggatatggat taactggttc
     9661 agatgttaaa ccagagcaat ttatggagaa agtattattt gctgtggatg aacttactgg
     9721 tttaattggt gctgctgcaa taatgcgtcc ttcaaagagc gtttctgatt tagaattaaa
     9781 atctgttaag aagaaattta aagataaatc atttgctgct ggctgttctc gagacgttat
     9841 aagaagtggt gcagaaatgc ttggatggga acttgataag cttatccaaa caactataga
     9901 agctatgaga agcttgattc cagagatggc aatatagctc ctccatacat ataccataga
     9961 agttacatct ttaaagagca tttctctttt tgatgcaact tctttttata ttttgtagaa
    10021 taaaaagttt aatattttaa actcactata taataaataa tataatgtat aatagtcata
    10081 aactttctaa ccttaaaagt ttctaataat caataaacaa ttct
//
LOCUS       DAMJVK010000124        10061 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.129, whole genome shotgun sequence.
ACCESSION   DAMJVK010000124 DAMJVK010000000
VERSION     DAMJVK010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10061
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.129"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(516..1673)
                     /locus_tag="PC414_003314"
     CDS             complement(516..1673)
                     /locus_tag="PC414_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HDF2465685.1"
                     /translation="MYLVISKKKAIFLGVILVIITAMVTSAFQLTLGNKVVISKELYE
                     DYKKYDKLLGLESIIKQDFYKKVSDTDLVNGAAKGLFLGTNDKYSGYYTKDEMENLIN
                     DSEGSYVGVGMYIGASKDGGLVVVPMKDSPAEKAGVKSGDKLVKVNGKSVSYKNSDEA
                     VRMMKGKKGKTVELTILREDKQLNFKVKTDQIIEKSIESKVIDNDLGYIEITQFISST
                     YTDFDKALKELKAKNIKGLVIDLRNNPGGMLDICKEVADELIGEGTIVYTKDNKGNTE
                     YLKSDKEKLGLPIVVLTNGESASAAEILTAAIVDNKEGISVGTTTFGKGLVQSVVRLK
                     DGTGYKLTTAQYFTPNGDYINEKGIKPTIEEKDENKQLDVATKWLREQIDK"
     gene            1827..2396
                     /locus_tag="PC414_003315"
     CDS             1827..2396
                     /locus_tag="PC414_003315"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HDF2465686.1"
                     /translation="MKKEFRKKVIESRKNKSANFVLSNSNLITEKLLQMEDIRNATNI
                     MLYLNFNNEVQTDNLIKKLLSLKKIVSSPITIKENHTLIPTQITDLKEGLRIGAYGIR
                     EPNEKSPTIDIKALDVVIVPAVAYDIHCYRLGYGGGFYDRFLENLRKDAITIGIAFDF
                     QIFDSIPKETHDAQLDYIVTETRIITPNN"
     gene            complement(2578..2994)
                     /locus_tag="PC414_003316"
     CDS             complement(2578..2994)
                     /locus_tag="PC414_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465687.1"
                     /translation="MNVKFNIKGIIYGVIALVFVIGGFIFIPSMFVEKSKPIDYTVLQ
                     RNSIPEKILDVMDKYTNEERALAAKIDNKIYIIVTRGNNKYGIEMDKIESVVEEGKDV
                     LRVKIKYKDKEDSFPYIVVETNMSELPDRIELNATK"
     gene            complement(3220..4314)
                     /locus_tag="PC414_003317"
     CDS             complement(3220..4314)
                     /locus_tag="PC414_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465688.1"
                     /translation="MSEKVNVKTVISFAGAYVATVIGSGFATGQEIMQFFSFYGFAGI
                     IGGIISMVLFSWMGASVMSKGKELQLKEPIKIYRVYCGKYLGIFFEWFGPLFLFGVFV
                     VMISGAGATLTEYYGLNPFVGRVGMAVVALLTVSLGLDKLSKILGGIGPIIIIFTLLV
                     GGISLAKNIGNIGEAAQVLSTVKIAKPVPNAYLSGVIYTTYNTIVVMAFLTGLGASAA
                     NKKEAICGGILGGVALMAAAIMMHLAILSDIGNLYSKAIPSLFLADKISPVIGVLFSI
                     ILILGIYTTAVPLLWSVTNRFVDDKHPKFKLITLIAAILGLIGGFLPFDKLVGILYPY
                     TGYMGVIILVCVLYRQITKTSGYQEGISDK"
     gene            complement(4737..5678)
                     /locus_tag="PC414_003318"
     CDS             complement(4737..5678)
                     /locus_tag="PC414_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HDF2465689.1"
                     /translation="MSKMATREAYGKALVKLGQINDNVVVLDADLSKSTKTHDFYKSF
                     PDRFFNMGIAEQNLIGAACGLSTAGKIPFASTFAMFATGRAFEIIRNSVCYPKLNVKI
                     CATHAGLTVGEDGASHESVEDIAIMRAIPNMTVLVPADGVETEKIIFEIAKYNGPVYV
                     RLGRSSVPVLFDEDYKFEIGKGTVLREGKDVSIIACGIMVNEALLAQEKLQEEGISAR
                     VINMSSIKPIDKDLILESAKETNAIVTVEEHSIIGGLGSAVSEVVGESCPTIVKKVGI
                     KDTFGESGTPNELLKKYELTCDDIIKTVKEVIIAKRM"
     gene            complement(5671..6498)
                     /locus_tag="PC414_003319"
     CDS             complement(5671..6498)
                     /locus_tag="PC414_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDF2465690.1"
                     /translation="MRDHKGLNEIARIIRRDIVSMIHRAKSGHPGGSLSVVEILTALY
                     FDEMNVDSSNPKMEDRDRFVLSKGHAAPALYATLAEKGYFDKEELNGLRKIGRMLQGH
                     PDMKGTPGVEISTGSLGQGFSVACGMAMASKLDNAPWNVYTLLGDGEVQEGIVWEAAM
                     SAAHYKLDNLIAFLDNNGLQIDGDIESVMSLGSIVDKFKAFGWNVIEIDGHDFDQIFA
                     ALDIAKSTVKKPTMIVAKTIKGKGISFMENQAGWHGTAPSDEELEKALLELGGADNE"
     gene            complement(6669..7052)
                     /locus_tag="PC414_003320"
     CDS             complement(6669..7052)
                     /locus_tag="PC414_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDF2465691.1"
                     /translation="MKGRLPKVSTNCEIKRGDLYYADLSPVVGSEQGGVRPVLIIQND
                     VGNKYSPTVIVSAITSQINKAKLPTHVEISSNEYGLNKDSVILLEQIRTIDKKRLREK
                     IGCLDENMMIKVDDGLQISLGLFTF"
     gene            complement(7021..7308)
                     /locus_tag="PC414_003321"
     CDS             complement(7021..7308)
                     /locus_tag="PC414_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HDF2465692.1"
                     /translation="MYDKSKEKIEVTLPDSLISEVDSIVQMENSNREDFAKAAFQFYI
                     TQKKKIHIKESMKTGYREMGQINLSLAELGMTVEEVSSDDKNEGKIAQGEY"
     gene            complement(7341..8498)
                     /gene="alr"
                     /locus_tag="PC414_003322"
     CDS             complement(7341..8498)
                     /gene="alr"
                     /locus_tag="PC414_003322"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDF2465693.1"
                     /translation="MQKITVPTWAEINLDNLRFNLNNIKNLLEEDIKICGVIKADAYG
                     HGAVEVAKLLEKEKVDYLAVARTAEGIELRQNGITLPILNLGYTPDEAFEDSIKNKIT
                     MTVYSLETAQKINEIAKSLGEKACVHVKIDSGMTRIGFQPNEKSVQEIIELNKLEYID
                     LEGMFTHFATADEVSKEYTYKQANNYKFMSDKLDEAGVKIAIKHVSNSAAIMDCPDLR
                     LNMVRAGIILYGHYPSDDVFKDRLELRPAMKLKSKIGHIKQVEPGVGISYGLKYTTTG
                     KETIATVPIGYADGFTRIQKNPKVLIKGEVFDVVGRICMDQIMVRIDKDIDIKVGDEV
                     ILFGEGEVTAERIAKDLGTINYEVLCMISRRVDRVYMENNELVQINSYLLK"
     gene            complement(8513..9103)
                     /locus_tag="PC414_003323"
     CDS             complement(8513..9103)
                     /locus_tag="PC414_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465694.1"
                     /translation="MRKKWTIVCIMFLALVIIVIGCQKRQSTKEEVYKDFQKQISDMN
                     YYSCKAEVEVVGNKSPHNYVLIHTYKKTDNYKLEVISPKHLKGKSIEYQDDKILVKNP
                     KISDVVELPNTGKNNQYLFVGDFIKNYLQNEEMKVKLSKGHLVLETFIPGDNKYFNKQ
                     VLYVNADTKNPEKMEVLDKEGVPRFTVKYKDFEYRN"
     gene            complement(9124..9585)
                     /locus_tag="PC414_003324"
     CDS             complement(9124..9585)
                     /locus_tag="PC414_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDF2465695.1"
                     /translation="MMDKTAKEIMTTDVIVAKQDDSIADVANMLIAEKIGGLPVVDSE
                     NRVVGIISETDILKKEKYIEAPLYINLLQGLIFLDDLKKVEKDIKQVAAYKVGELMSK
                     DIIKVHEDDKFDDVANVMIKKSINRVPVVDDDNKLKGIICRYDIIKALYNE"
     gene            complement(9600..9980)
                     /locus_tag="PC414_003325"
     CDS             complement(9600..9980)
                     /locus_tag="PC414_003325"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HDF2465696.1"
                     /translation="MNIFDIGVDIIEIDRIRKAVDKNNRFLEKIFTDREIEYFNSKNF
                     KAESIAGNFAAKEAISKSIGTGIRLFNFKDIEVLRNEMGKPIVKTYNNLAKMCIDYNV
                     LEIKVSISHSKDYAIANAITIIKD"
BASE COUNT         3237 a         1860 c         1061 g         3903 t
ORIGIN      
        1 attcacatgt tttaggaata aattatataa aataggttat aagataatga acatttatat
       61 tataacctat ttttatatcg ataagtatga aattgtttaa ccaagtcaaa atgatatata
      121 aatttatatt taaatgtgtt tagagtttaa tttattttca atgtttaaaa aagttattct
      181 atgcatctaa tttaattagt aaactgaggt tctagttaga taaattatat tataatgaat
      241 atatagtatg cagtttacga tattttaaaa gtaaatgata taatatttgg aacaactggg
      301 ttgcaatgtg tttttaaaaa atatcaaaat aaggctatga tttttaaaac catagccctg
      361 tctgtaaaaa tttaattatc acttaaaaaa taaattttta tttaaagaat acacataaat
      421 atttccagac tccctcatga actaaaaagt tcattttgag acatctcttt tataatgttc
      481 tttattttat agtataagtt tataaaaatt aaatcctatt tatcaatctg ttctctaagc
      541 cacttagttg ctacatctaa ctgtttattt tcatcttttt cttcaatagt aggctttata
      601 cctttttcat tgatataatc tccattaggt gtaaaatatt gagcagtagt taacttgtat
      661 cctgttccat ccttcaatct tacaactgat tgtaccaacc ctttaccaaa agttgtagtc
      721 cctacagaaa taccttcttt attatcaacg attgcagctg ttaatatttc agcagcagaa
      781 gcactttctc cattagttaa cactactatt ggaagtccca atttttcttt atcagatttc
      841 aagtactcag tatttccctt attatctttt gtatatacta tagttccttc accaattaac
      901 tcatcagcaa cttctttaca tatatctagc attccaccag gattatttct aaggtctatt
      961 actaatccct tgatattttt tgcttttaat tcttttaaag ctttatcaaa gtcagtatat
     1021 gtacttgata taaattgggt aatttctata tatcctaaat cattatcaat tactttactt
     1081 tctatactct tctcaattat ctggtctgtt tttactttaa agttcaattg tttatcttct
     1141 cttaatatag tcagttcaac tgttttacct tttttaccct tcatcatacg aacagcttca
     1201 tctgaatttt tatatgagac agattttcca ttgactttta ctaatttatc ccctgatttt
     1261 acacctgctt tctctgctgg agaatctttc atgggaacta ctactaagcc accatctttt
     1321 gatgccccta tatacatacc tacaccaaca taagaacctt cactatcatt aattagattc
     1381 tccatttcat ctttagtata ataaccagaa tacttatcat ttgttcctag aaatagacct
     1441 tttgctgctc catttaccaa atcagtatct gatacttttt tataaaaatc ttgttttata
     1501 atagattcta gtcctaacaa cttatcatat tttttatagt cttcatagag ttctttcgat
     1561 attacaactt tattccccaa tgtcaactga aatgctgatg taaccatagc tgttataatt
     1621 actaaaatca cccctaaaaa aatagctttc ttctttgaaa tcacaagata catctccttt
     1681 tacataaact attattatta tatgactagc tgttattttt attacattta cccaaatata
     1741 ttgctttatt acaataaatt atactaaaat ttatattaaa tacaattgct gtacacatta
     1801 taacatatta agggaggaat tattttatga aaaaagaatt tagaaaaaaa gttattgaat
     1861 caagaaaaaa taagagtgca aacttcgtac ttagtaattc aaatttaatc actgaaaaat
     1921 tacttcaaat ggaagatata agaaatgcaa ctaatataat gctttatcta aattttaaca
     1981 atgaagttca aacagataat ttaatcaaaa aacttttgtc tctaaagaaa atagtatcaa
     2041 gtcccatcac aataaaagaa aatcatacat taatccctac tcaaataaca gatttaaaag
     2101 agggattgag aattggagca tatggaataa gagaacctaa cgaaaaatca cctacaattg
     2161 atattaaggc cttagatgtt gtaattgtac cagctgtagc atatgatata cattgctata
     2221 gattaggata tggtggagga ttttatgata gatttttaga aaacttaaga aaagatgcta
     2281 taactatcgg catagcattt gacttccaga tattcgattc tattccaaaa gaaactcatg
     2341 atgcacaatt agactacata gttactgaaa ctagaattat cactccaaat aattagaata
     2401 ttaagtattt ccagacccca tttaatatag taataaatac tactgttaat atcttacttt
     2461 ataagcattt taagtcatgg cttttacaac aataagaacc atatacaatt tacttacatt
     2521 aataaaaacc atgactttat tgtacaatat gcagttattt aattataaaa taccttctta
     2581 tttcgtcgca tttaattcta ttctatctgg aagttcactc atatttgttt caacaactat
     2641 gtatggaaaa gaatcttcct tatctttata ctttatcttg actctcaaaa catcctttcc
     2701 ttcctcaacc acactttcta ttttgtccat ttctatacca tatttattat ttcctctagt
     2761 cactataatg tatattttgt tatcgatttt agccgctaat gctctttctt catttgtata
     2821 cttatccatc acatctaata tcttttctgg tattgaattt ctttgtaata ctgtataatc
     2881 tatgggttta gatttctcta caaacatact tggtatgaat ataaatcccc ctataacgaa
     2941 tacaagagct ataactccat aaattatacc tttaatatta aactttacat tcaatcttat
     3001 cactcctccc atatattcta aatatatgat ttataaactt atcttagact ctaaattatt
     3061 gattagtata tatttataca taattataaa tatatgtaat gtaactaaaa aacatgtttt
     3121 ttctatttca taataaacga gctaaaaaat aaaaagagct atatgaaaac attttcattg
     3181 ttttcatata gctcttgagt ttacaatatt aaaaaatact tatttgtcac tgattccttc
     3241 ttgatatcca gatgtttttg taatctgtct ataaagaaca caaaccagta ttatgacgcc
     3301 catatatcca gtatatggat ataaaatacc aaccagctta tcaaatggta ggaagccacc
     3361 tataagacct aatatagctg ctattaaagt tattaattta aacttaggat gtttatcatc
     3421 tacaaatcta ttagttactg accataataa aggtacagct gttgtatata tacctaatat
     3481 aagaattatt gagaataaaa ctccaattac tggtgaaatc ttatctgcta agaataatga
     3541 tggtattgct tttgaatata agtttcctat atcagaaaga atagctaagt gcatcattat
     3601 agctgcagcc ataagtgcta ctccacctaa tataccacca caaatagctt cttttttgtt
     3661 agcagcactt gctcctaatc cagttaagaa tgccataaca acaattgtgt tataagtagt
     3721 atatattaca ccagatagat aggcatttgg aactggttta gctatcttta cagtgctaag
     3781 aacttgtgca gcttctccta tattacctat gtttttagct aaacttatac caccaactaa
     3841 aagtgtaaag attattatta taggccctat tccacctagt atctttgata atttatcaag
     3901 acctagtgaa acagttaata atgctactac tgccatacct actctaccta caaatggatt
     3961 taatccatag tattcagtta aagttgctcc tgctcctgaa atcattacta cgaaaacacc
     4021 aaataagaat aaaggtccaa accattcaaa gaatattcct aaatacttac cacaataaac
     4081 acgataaatt ttgataggtt ctttaagttg aagctcttta ccttttgaca taactgaagc
     4141 acccatccat gagaataata ccattgaaat tatcccacca ataatacctg caaaaccata
     4201 aaaagagaag aattgcatta tttcctgtcc tgttgcaaat ccagaaccga ttactgttgc
     4261 tacatacgca cctgcgaaac tgatgactgt tttaacattt actttttctg acatatctca
     4321 gttacctccc catgtgaaat tttttcttct gattaaacaa ttcgataaac tacctacaat
     4381 ttcctttaac ataaaataca tgataactgt tttatagctt tttagtataa taacttaaaa
     4441 cttataatct gattttatac cctactcctc ctctcataat ttttttagat tcttttatct
     4501 aagtagtcat tatatataaa attagtttct ctataaatta tatattagtc atatttttct
     4561 gaatagtcaa tatatatttg aatattttct ctatttttgt aaaaatttga atattttatt
     4621 acatttttga tatattgata tcatataaaa aaaagagaga tataaattaa tataacactt
     4681 tatacctctc ttttaaatat attatattat taacaaacat gaataattta tatctcttac
     4741 attctttttg caataataac ttctttaaca gtctttatta tgtcatcaca agttaattca
     4801 tattttttta acaattcatt tggagtacca gattctccaa atgtatcttt aattccaact
     4861 ttctttacaa tggttggaca agactctcct actacctcac ttacagcaga acctaatccc
     4921 ccaattatgc tatgttcttc tacagttaca atagcattcg tttcttttgc tgattctaat
     4981 attaaatctt tgtctatagg ttttatcgag gacatgttta taactctagc acttatacct
     5041 tcttcttgca acttttcttg tgctaataga gcttcattta ccataattcc acaagctata
     5101 attgatacat cctttccttc tcttaagact gtaccttttc ctatttcaaa tttataatct
     5161 tcatcaaata aaactggtac tgaacttctc cctaatctaa cataaacagg tccattatac
     5221 ttagctattt caaaaataat tttttctgtt tcaactccat cagctggaac aagaactgtc
     5281 atatttggta tagctctcat tatagcaata tcttccacac tttcatgaga tgctccatcc
     5341 tctccaacag taagacctgc atgagtagca catattttta catttagctt tggataacaa
     5401 actgaatttc ttataatctc aaaagccctt cccgtagcaa acatagcaaa agtacttgca
     5461 aaaggtattt taccagcagt tgataatcca caagcagcgc ctattaaatt ttgttcagct
     5521 atacccatat taaaaaatct atctggaaat gacttgtaaa aatcatgtgt ttttgtagac
     5581 tttgataaat ctgcatctaa tactacaaca ttgtcattta tttgacctaa ttttactaat
     5641 gctttaccat atgcttctct cgtcgccatt ttactcattg tcagcacctc ctaattcaag
     5701 caaagctttt tctagctcct catcacttgg agctgtccca tgccatcctg cttgattttc
     5761 cataaaagat attcctttgc cttttatagt ttttgcaact atcattgtag gttttttaac
     5821 tgttgattta gctatatcaa gagccgcaaa tatttggtca aaatcatgtc catctatctc
     5881 aattacattc caaccaaaag ctttaaattt atcaactatt gaacctaaac tcattacgct
     5941 ttctatatct ccatctattt gaagtccatt attatctaaa aaagcaatta aattatcaag
     6001 tttataatgt gcagcactca tagcagcttc ccaaacaatt ccttcttgaa cctcaccatc
     6061 accaagcaaa gtatacacat tccacggagc attatccaac tttgatgcca tagccatacc
     6121 acatgcaact gaaaaacctt gacctaaaga accagtagat atttccacac ctggcgtgcc
     6181 tttcatgtct ggatgacctt gtaacatcct tcctatcttc cttaatccat tcagttcttc
     6241 cttatcaaaa tagccttttt cagccaatgt agcatataat gctggtgcag catgtccttt
     6301 tgataataca aatctatctc tatcctccat tttaggattt gaagaatcca cattcatttc
     6361 atcaaagtat aacgcagtaa gtatttcaac tactgataat gaccctcctg gatgtccaga
     6421 tttggctcta tgtatcattg aaactatatc tcttcttatg atacgcgcaa tctcatttaa
     6481 tcctttatgg tctctcatgc aattacctcc cttgagttcg taattttata tcaattccat
     6541 attattatat cacttttatg ctattttttt gcgttatttt taattttatt aacatagtac
     6601 attaaaatta aaccttaaga gatatttaaa agaaaaaagg gattgtctaa taaaagacaa
     6661 atccctcttt aaaaagtaaa caatcctaaa cttatttgaa gtccatcatc tacttttatc
     6721 atcatatttt catctagaca accaattttt tccctcagtc tctttttatc gatagttcta
     6781 atttgctcta aaagaattac tgagtcttta ttaagcccat attcattgga acttatctct
     6841 acatgagtag gtaatttggc tttattaatc tgagatgtta tagctgatac tataactgta
     6901 ggactatatt tattacctac atcattttgt attatcagaa caggtctaac gcctccctgc
     6961 tctgaaccaa caactggact taaatcagcg taatataaat caccacgctt tatttcacaa
     7021 ttagtactca ccttgggcaa tcttcccttc atttttatca tcagaagata cttcttcaac
     7081 tgtcatacct aattcagcta gtgacagatt aatttgaccc atttctctat aacccgtttt
     7141 catagattct ttaatatgaa ttttcttttt ctgagttata tagaactgaa aagcagcttt
     7201 tgcaaaatct tctctgttac tgttctccat ctgaactata ctatcaactt cagaaatcag
     7261 agagtcaggt aaagtaactt ctattttttc tttactctta tcgtacacaa aatcgcctcc
     7321 cagattataa tatcatggtt ttattttagc aaataactgt ttatttgtac aagctcatta
     7381 ttttccatat aaacacggtc aactcttctt gatatcatac ataacacttc atagtttata
     7441 gttcctaagt ctttagctat acgctcagct gtaacttcgc cttctccaaa tagtataacc
     7501 tcatctccaa cttttatgtc tatatctttg tcaattctaa ccattatttg atccatacat
     7561 attctaccaa ctacatcaaa cacttctccc ttaataagaa cctttggatt tttttggatt
     7621 ctagtaaatc catctgcgta tcctattgga actgtagcta ttgtttcttt acctgtagtt
     7681 gtgtatttta gtccataact tattcctaca cctggttcaa cttgttttat atgtccgatt
     7741 tttgatttta atttcatggc tggtcttaat tctaatctat ctttaaatac atcatcagat
     7801 ggataatgac catataatat tattcctgct cttaccatat ttaatcttaa atcagggcaa
     7861 tccataatag ctgcactgtt tgatacatgt tttatagcta tttttacacc agcctcatct
     7921 aatttatcag acataaattt ataattatta gcttgtttat aagtgtactc tttacttact
     7981 tcatcagctg tagcaaaatg agtaaacata ccttctaaat ctatatattc taatttattt
     8041 aattctatta tttcctgtac tgacttctca ttaggttgga aacctattct agtcatccct
     8101 gagtctattt taacatgaac acaggctttt tctcctaaag attttgcaat ttcatttatc
     8161 ttttgtgctg tttctaaaga ataaactgtc atagttattt tattttttat agaatcttca
     8221 aaagcttcgt ctggagtata tcccaagttc aaaataggaa gtgttatgcc attttgtcta
     8281 agttcaattc cttcagcagt tcttgctact gctaagtaat ctactttttc tttttctagc
     8341 aattttgcaa cttctactgc accatgtcca tatgcatcag cttttattac tccacaaatc
     8401 ttaatatctt cttctaataa atttttaata ttatttaagt taaatcttaa gttatctaga
     8461 tttatctctg cccatgtagg cactgttatt ttttgcatgt ctttagtccc ccttagtttc
     8521 tgtattcaaa atctttgtat tttactgtaa atcttggcac tccctcttta tctaacactt
     8581 ccattttttc aggatttttt gtgtcagcat ttacatataa tacttgcttg ttaaaatatt
     8641 tattatcccc aggaataaaa gtctctaaaa ccaaatgtcc tttggataac tttactttca
     8701 tctcctcatt ttgaagatag ttttttataa agtctcctac aaaaagatat tgattatttt
     8761 ttcctgtatt aggtaactcc actacatcac taatctttgg atttttaact aatattttat
     8821 catcttgata ttctatactt ttccctttta aatgttttgg ggaaattact tctaatttat
     8881 agttatcggt ttttttataa gtatgaatta aaacataatt gtgtgggctt ttatttccaa
     8941 ctacttctac ttcagcttta caactgtaat aattcatatc agaaatctgc ttttgaaaat
     9001 ctttgtatac ttcttctttt gtggactgtc gtttttggca tcctattaca attataacca
     9061 gtgccaagaa cataatacat actatggtcc acttttttct cacatcacac ctcctacttc
     9121 agtttattca ttgtataaag cttttattat atcatatcta caaattatac ctttaagttt
     9181 gttgtcatca tctactacag gaactctgtt tattgatttt ttaatcatta cattagctac
     9241 atcatcaaac ttatcatctt catgaacctt tattatatct ttagacataa gttctccaac
     9301 cttataagca gctacttgtt ttatatcttt ttcaactttt tttaagtcat ctaagaaaat
     9361 caatccttgt aatagattta tatagagagg tgcttctata tatttttctt ttttaagaat
     9421 atctgtttca gaaattattc caacaactct attctctgaa tctacaactg gcagacctcc
     9481 tattttttct gcaattagca tattagctac atctgctata ctatcatctt gttttgctac
     9541 tattacatct gttgtcatta tttcttttgc tgttttatcc atcataatgt aatcactcct
     9601 tagtccttaa ttatggttat cgcatttgct atagcataat cttttgaatg tgatattgaa
     9661 acttttattt ctaaaacatt ataatctatg cacatttttg ctagattatt ataagttttt
     9721 actattggtt ttcccatttc atttctgaga acttctatat ctttaaaatt aaacaatcta
     9781 attcctgtac ctatagattt acttatagct tccttggctg cgaaattacc tgctatactt
     9841 tctgctttaa aattcttaga attaaaatac tctatttctc tatctgtaaa tatcttctcc
     9901 aaaaacctat tatttttgtc tacagccttt cttattctat caatttcaat tatatctacg
     9961 ccaatatcaa atatattcat tgttcctcca gaaaatttat aatacttctt tcaataatta
    10021 ataatgaatt tataaattta ctatcgataa tacacattat t
//
LOCUS       DAMJVK010000125         9822 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.130, whole genome shotgun sequence.
ACCESSION   DAMJVK010000125 DAMJVK010000000
VERSION     DAMJVK010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9822
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.130"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            538..1446
                     /gene="trxB"
                     /locus_tag="PC414_003326"
     CDS             538..1446
                     /gene="trxB"
                     /locus_tag="PC414_003326"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDF2465697.1"
                     /translation="MVDIIVIGAGPAGLTSAIYAMRAGLSVTVFEKNIYGGQVASTSE
                     VENYPAVQKISGVEFSNNIYNQAVAQGVDIQFDEVEEINLEGKVKVVKTSSGEHKAKA
                     VILANGVERRKLGCAGEQEFTGRGVSYCATCDGAFFKDKEVAIVGGGNTALEDALFLA
                     NNCTKVYLIHRRDSFRGEEVLEKSVKARENIEILYSHGVEKIEGEKTVSKIEVKNLKT
                     EEKRTIDVSGIFIAIGLKPNNKMFENVLDLDEGGYIISDESCITSVEGVYVAGDSRTK
                     FLRQIITAASDGAIAAVQAANYINVE"
     gene            complement(1536..3359)
                     /gene="typA"
                     /locus_tag="PC414_003327"
     CDS             complement(1536..3359)
                     /gene="typA"
                     /locus_tag="PC414_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HDF2465698.1"
                     /translation="MSQKHKIINIAVIAHVDAGKSTLVDAFLSQSGVFRKNEVVKDCV
                     MDSNDLEKERGITIYSKNCAINYEDYKINIVDTPGHSDFSSEVERVMKTVDTVILLVD
                     ASEGPMPQTRFVLQKSLEFGLKPILFINKIDKKDQRAEEVVNEVFDLFVDLNATDEQC
                     EFPIIYGIAKQGIAKLEMDDDSEDLSPLFKTIVNHVEAYPNYDNEPLQFQISALAYDD
                     YVGRLGIGRIYKGTLKNNTQVAICREDSVVSKGKVSKLSVYEGLKQVEVDEATSGEIV
                     VIAGIPDISIGETICDLDSPLPMEMIKIEEPTLSMNFLVNDSPFVGKSGKFVTTRHLK
                     DRLEKELEVNVGLKVEPLDTTDGYKVSGRGELHLSILLENMRREGYEVGVSKPEVLMH
                     KEDGKLMEPIERVVVNCPEVYSGTIINELNMRKGMMESMSIEGDYVKIEFLAPTRGLL
                     GYRSEFINATRGEGTLVRSFEKFEEFKGEIPSRGNGVLIAQGPGVTMGYSLNALSDRA
                     VMFVDPGVEVYEGMIIGMNSRKDDMVVNPCKNKKMSNVRASGSDDAIKLSPPRIFTLE
                     EALEFIEDDELVEITPDSIRLRKRFLNEHDRLRYNKSRQGK"
     gene            3571..3813
                     /locus_tag="PC414_003328"
     CDS             3571..3813
                     /locus_tag="PC414_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465699.1"
                     /translation="MLEVTKIIIKYFASESDTFVKEEVCNFEELLKWSHKFIEGKKIE
                     DVLYIKSKENYKVNVIKSLEIKELEIVRKQQIPPYM"
     gene            3961..4152
                     /locus_tag="PC414_003329"
     CDS             3961..4152
                     /locus_tag="PC414_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1450 domain-containing protein"
                     /protein_id="HDF2465700.1"
                     /translation="MIRVCPNCSNVDVNKLKTLVGEENVKTGCIGQCRAFKKEAVGII
                     DNELEIKETEAEFFEACKE"
     gene            4436..6937
                     /locus_tag="PC414_003330"
     CDS             4436..6937
                     /locus_tag="PC414_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HDF2465701.1"
                     /translation="MSSNKVVESYKITGMTCAACAKAVERVTKKMDGVYDQSVNIATE
                     KLKIEYDNSKVNFDDIKQVVEKAGYGIIKEESNKKIDMKIDGMTCAACAKAVERVVKK
                     LDGVESISVNIATDKANIDYDPSKVKLSQIKAAIEKAGYKPIEEVKNKVDVDEDKLRK
                     EREMKSLFVKFIVAIVFAVPLFYIAMGPMIIKPIGPWPLPEIINPMTNTFNYALIQLI
                     LVIPVMIAGYKFYINGFKSLFSLSPNMDSLVAIGTLAAFLYSLYTTLQIANGQIQGMH
                     HHQLYYESAGIIIALILLGKYLESKSKGKTSEAIKKLMGLQPKTAIVLVDGKEVETPI
                     EEVEIGDILLVKPGTKIPVDGVVIEGYTSVDESMLTGESIPVEKNVGSKVTGASINKN
                     GVIKFKAEKIGGDTALAQIIKLVEDAQGTKAPIAKLADTVSGYFVPIVIAIAVVASLL
                     WFLIGGKDIVFVLTIFISVLVIACPCALGLATPTAIMVGTGKGAENGILIKGGEALES
                     AHKVNTVIFDKTGTITEGKPKVTDIVLNNNVKEEYLIKIASSAEKGSEHPLGEAIVKY
                     GEEKNIKFEKVDNFKAIPGAGIQVTINDESILLGNRKLMNDNNIKLGDLEEKSNILAS
                     QGKTPMYIAVDGNLSGIIAVADVVKESSKKAIEILHDMGIKVAMVTGDNAKTANAIAN
                     QVGIDMVLAEVLPEDKSKEVEKLQNQGKFVAMVGDGINDAPALAKADIGIAIGSGTDV
                     AIESADIVLMKSDLIDVPTAIKLSHETIKNIKQNLFWAFGYNTIGIPVAAGILYVFGG
                     PLLNPMIAAAAMSLSSVSVVSNALRLKNFKAYKRD"
     gene            7097..7765
                     /locus_tag="PC414_003331"
     CDS             7097..7765
                     /locus_tag="PC414_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2465702.1"
                     /translation="MNSSILVIEDDSNIQELISEFLSAEGYQVDTANDGLEGIQKFKQ
                     GSYDLVILDIMMPNLDGYGVCKMIRKSSSVPIIFLTALNDEGDQLKGFDLECDDYITK
                     PFSFNLLIKRVEAILRRSNKTINDKFIVFEKLKLDLNTYIAEIDGEPIELTLKEFNIL
                     KALIEKYPQVITREGLLDSIWGYDYYGDTRIVDAHIKNIRKKISLPYIKTVKGIGYTL
                     EKDI"
     gene            7749..9026
                     /locus_tag="PC414_003332"
     CDS             7749..9026
                     /locus_tag="PC414_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HDF2465703.1"
                     /translation="MRRIFDKWEKLSIKYKLFSITTSLLIALALIIYLILYFLLPSYY
                     HEYKIESLQESLKSLVDSSIHFDTYTLEERLYYMAKDQNLAILLKDNQGKIVYGKNEV
                     VILRYSKYMINSLEDEYRTSIPIYTKDAKDGPYTLELVMPLQPIDEANEVIRKLMPYI
                     ISIAILIAIIGAYIYSIVITKPLINIIESEREQEYRRKDFVATISHELKTPITIISGQ
                     IEGMIYSVGKYKDRDTYLKKSYECTQELKDLVNEMIEVSKSEILEKDLKLVSINISEL
                     LNRLVKRQVFLIEEKHMKTILKIEENLEIKADQERITKAINNIINNAIKYSPEESELI
                     IRLYDKNKRISKKTSNQRVVLEIENTGVTIEKRYLEEIFNPFYRIEKSRSRKTGGSGL
                     GLYIVSQIFKSHGFDYSIKNKENSVVFTVEFKN"
     gene            9427..9648
                     /locus_tag="PC414_003333"
     CDS             9427..9648
                     /locus_tag="PC414_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465704.1"
                     /translation="MDRLKRDMKNMKEDLKEGVKKTAYVMKNTAEDLKDDAQGAMVNL
                     EMKKDEMMERYEQKKFSKELEKQMKNEMK"
BASE COUNT         3912 a         1108 c         1658 g         3144 t
ORIGIN      
        1 ttacaaaact ttaatttgaa aaatgtaatt atatggtata tcatataaat ataagtaaat
       61 agtgctaata tttaaactat taatcataat tggttcaaca aaagtatata gttaataatg
      121 tttaatttga agaaattgtt agtgtgaaat tacatatata ttcaaattaa attcataaat
      181 atttcacata ataatagtat actgtaaaca aaaggaggta ttaaatatgg gaatagtact
      241 tataacagca gcattagttt tggttatgta tggactttca attctatcat atatatcaga
      301 aagcaatata ttagaagaca ctgtaaatga taaccaataa ggttttgaat tatataaata
      361 agtgagaact gtaaataaaa gttaattttt atttgcagtt tttttatata tatgtaaaat
      421 aaagttaatt aataaaaaat aatgttttgt ttatagtata tatggataat aatagtaaag
      481 tataagaatg atgagaataa tatataagaa ataataatat aaagagaggt aaacgttatg
      541 gtagatatca ttgtaattgg tgctggacca gcaggtttaa cttcagcaat ttatgctatg
      601 agagcagggt taagcgttac agtatttgaa aaaaatattt atggaggtca agttgcaagt
      661 acctctgagg tagaaaatta tccagcagtt caaaagattt ctggggtaga attttctaat
      721 aatatataca atcaagcagt tgctcaaggt gttgatattc agtttgatga ggttgaagag
      781 ataaatttag agggaaaagt aaaggttgta aaaacttctt caggtgaaca taaagcaaag
      841 gctgttattc ttgcaaatgg agttgaaaga cgtaaattag gttgtgcagg ggaacaagaa
      901 tttacaggta gaggagtatc ttactgtgca acatgtgatg gagccttttt taaagataaa
      961 gaagttgcta tagtaggtgg aggaaatact gcactagaag atgctttatt tttagctaat
     1021 aattgtacaa aagtgtattt aattcacaga agagatagtt ttagaggtga agaagtgttg
     1081 gagaaatctg tgaaagcaag agagaatatt gagatacttt acagtcatgg tgtagaaaaa
     1141 atagaaggag aaaagactgt atcgaaaata gaagtaaaaa acttaaagac tgaagaaaaa
     1201 agaactatag atgttagtgg tatttttatt gctataggtc ttaaacctaa taataaaatg
     1261 tttgaaaatg ttcttgattt ggatgaaggt ggatatataa tttcagatga gtcttgtata
     1321 acatcagtag aaggtgtata tgtagcagga gatagtagaa ctaagttttt acgtcaaata
     1381 ataacagcag catcagatgg agctattgca gccgttcaag cagctaatta tataaatgtt
     1441 gaataatata aatttcaaat aaaaatagcc atataaactg aaataatttt tcagtactat
     1501 atggctattt tgttaattag aggtaaatgt acgttttatt taccttgtct agatttgtta
     1561 tatcttaatc tatcatgttc atttaagaat ctttttctaa gtcttataga atcaggtgta
     1621 atttcaacta attcatcatc ttctataaac tctagtgctt cttcaagagt aaatattcta
     1681 ggaggagata attttatagc atcatctgaa ccagaagcac gtacgttact cattttcttg
     1741 tttttacaag ggttgactac catatcatct tttctggaat tcataccaat tatcatacct
     1801 tcgtaaactt ctacaccagg gtctacaaac attacagctc tatcacttaa ggcattaaga
     1861 gaatacccca tagtaacacc aggtccttgt gctattaaaa caccatttcc tcttgaaggt
     1921 atttctcctt taaattcttc aaatttttca aatgaacgaa ctaaagtacc ttctccacga
     1981 gtagcgttta taaattcact tcgatatcct aaaagaccac gagtaggtgc taagaactct
     2041 atttttacat agtctccttc tattgacata gactccatca taccttttct catgtttaat
     2101 tcatttataa tagtaccaga gtaaacttca ggacagttta caacaactct ttctattggt
     2161 tccattaatt ttccatcttc tttatgcatt aaaacttcag gtttagaaac accaacttca
     2221 taaccttcac gtctcatatt ttcaagtagt atagataagt gaagctctcc acgtccagaa
     2281 actttgtatc catcagtagt atcaagaggt tcaactttaa gaccaacatt tacttcaagt
     2341 tccttttcaa gtctatcttt caaatgtcta gttgtaacga attttccact ttttccaacg
     2401 aatggagagt cgtttactaa gaagttcata gaaagagtag gctcttctat ttttatcatt
     2461 tccataggta gtggactatc taaatcgcat atagtttcac ctatagatat atcaggaata
     2521 ccagctataa caactatttc tccactagta gcttcatcaa cttcaacttg ttttaaacct
     2581 tcatacacag aaagttttga tacttttcct ttagatacaa cagaatcttc tctacatata
     2641 gcaacttgtg tattgttttt aagagtacct ttgtaaattc tacctatacc aagtctacct
     2701 acataatcat catatgcaag agctgatatt tggaactgta gaggttcatt atcgtaatta
     2761 ggataagctt caacgtgatt tacaatagtt ttaaatagag gagaaaggtc ttcactatca
     2821 tcatccattt caagtttagc aataccttgt ttagcaatac catatattat aggaaattcg
     2881 cattgctcat ctgttgcatt taagtctaca aataaatcga aaacttcatt aactacttct
     2941 tccgctctct ggtctttttt atcaatcttg tttataaata atattggttt taaaccgaac
     3001 tctaaagatt tttgtaaaac gaatctagtt tgaggcattg ggccttcact agcatcaact
     3061 aatagaataa ctgtgtcaac agttttcata acacgttcaa cttcagaaga aaagtcactg
     3121 tgtcctggag tatcaactat gtttattttg taatcttcat agtttattgc acagtttttt
     3181 gaatatatag ttatgcctct ttctttttca aggtcgttac tgtccataac gcagtcttta
     3241 actacttcgt tctttctaaa aacaccgctt tgagaaagga aagcatcaac caatgttgat
     3301 tttccagcgt caacgtgggc tataactgcg atatttataa ttttatgttt ttgtgacatt
     3361 tttgttgctc ccttcgatta aaataactta ttaattatag cacaatatat tattaggtac
     3421 aaggaggaat atattaaatt gtattaatat agtttaaatt gaataaaact gttagttttg
     3481 catctgaaaa ttattttgtt taaaaaaatt ttttagacaa ataataacct taatagataa
     3541 aaagattaat aaaagagtaa aggatgatat atgttggaag taaccaaaat aataattaaa
     3601 tattttgcaa gtgaaagtga tacttttgta aaagaagaag tttgtaattt tgaagaactt
     3661 cttaagtggt ctcataaatt tattgaaggt aaaaagatag aagatgtttt gtatataaag
     3721 tctaaagaaa actataaagt aaatgtgatt aagtctttag aaattaagga attggaaata
     3781 gtgagaaaac aacagatacc accgtacatg tagtattaat aatatataaa attaatatgt
     3841 aatactcgca ataggatata aatggtatat atggtaataa tttatattaa aaaaactttt
     3901 ataaagttta tatttgggta taaaagtatt aattagacat taattaggag gaacttaaat
     3961 atgataagag tatgtccaaa ctgttcaaat gtggatgtaa ataagctaaa aactttggtt
     4021 ggagaagaaa atgtaaagac tggttgtata ggtcagtgta gagcatttaa aaaagaagct
     4081 gttggaataa tagacaatga gcttgaaata aaggaaacag aagcagagtt ttttgaagca
     4141 tgtaaagaat aaattttaaa taaaatataa taaatagagc atctaaattg tataagcatt
     4201 aaacaattta gatgctctat ttttatatat acatttaatt atgagtatac ttttctatta
     4261 aatacaaata aatataacta atacatttat ttattaaaaa aatattgatt taatatacct
     4321 atagggggta taataaaaat atagatttct aaataagtaa tttatcaata ttacatatca
     4381 aaataaaacg ttacaaatta gctgaaaata tattgaaaga aaggaagatt ttattatgag
     4441 ttcaaataaa gtggtagaaa gttataaaat tacaggtatg acatgtgcag cttgtgcaaa
     4501 ggcagtagag agagtaacta agaagatgga tggagtatat gaccaaagtg taaatatagc
     4561 tactgaaaaa cttaaaatag aatatgataa ttctaaggtt aattttgatg atataaagca
     4621 ggttgtagaa aaagcaggat atggaattat aaaggaagaa agtaataaaa aaattgatat
     4681 gaaaattgat ggaatgacat gtgcagcttg tgcaaaagct gtagagagag tggtaaaaaa
     4741 attggatgga gtagaaagta ttagtgtgaa tattgcaaca gataaagcta atatagacta
     4801 tgacccatca aaagtaaaat tatctcaaat aaaagcagct attgaaaaag ctggatataa
     4861 accaatagaa gaggtaaaaa ataaggttga tgtggatgaa gataaattga gaaaagaaag
     4921 agaaatgaag tctttatttg ttaaatttat agttgcaata gtatttgcag tacctctgtt
     4981 ttatatagca atgggaccta tgataataaa gcctataggg ccatggcctt tgccagaaat
     5041 tataaatcct atgacaaata cttttaatta tgcattaatt cagttaattt tagtaatacc
     5101 agtaatgata gctggttata aattttatat aaatgggttt aaatcacttt tttctttaag
     5161 ccctaatatg gattctttag ttgcaatagg aacattagcc gcatttttat atagtctata
     5221 tacgacacta caaatagcca atggacaaat tcaaggtatg caccatcatc agttgtatta
     5281 tgaaagtgca ggaataataa tagcacttat cttacttggt aaatatttag aatcaaaatc
     5341 aaaaggaaaa acatcagaag caattaaaaa gcttatgggg ctacaaccta aaactgcaat
     5401 agtgttagtt gatggtaaag aagttgaaac tcctatagaa gaagtagaga taggagatat
     5461 actattagta aaaccaggaa ctaaaatacc tgtggatgga gttgtaatag aaggatatac
     5521 atcagttgat gaatcaatgc ttacaggtga aagtatacca gtagaaaaga atgttggaag
     5581 taaggtgaca ggagctagta ttaataaaaa tggtgttatt aagtttaagg ctgaaaaaat
     5641 tggtggagat actgctcttg cccaaataat aaagttagtt gaagatgcac aaggtacaaa
     5701 agcacctatt gctaaacttg ccgatactgt atctggatat tttgtaccaa tagtaattgc
     5761 aatagctgta gttgcatcat tattgtggtt tttaatagga ggaaaagata tagtatttgt
     5821 acttactata tttatatcag tacttgtaat tgcttgtcct tgtgcattag gattagcaac
     5881 accaactgct ataatggttg ggactggaaa gggcgcagaa aatggaatct tgataaaagg
     5941 tggagaggca ttagaatctg cacataaagt aaatacagtg atatttgata aaacaggtac
     6001 tataacagaa ggaaagccaa aagtaacaga tatagtatta aataataatg taaaagaaga
     6061 atatcttatc aagattgctt caagtgcaga aaaaggttca gaacatcctc ttggagaagc
     6121 aatagttaag tatggagaag agaagaatat aaaatttgaa aaagttgata attttaaagc
     6181 cataccaggt gctggtatac aagttactat taatgatgaa agtatattac ttggaaatag
     6241 aaagcttatg aatgacaata atataaagct tggggattta gaagaaaaat caaatatatt
     6301 ggcaagccaa ggtaaaacac ctatgtatat agctgtagat ggaaatttat caggtataat
     6361 agcagttgca gatgtagtca aagaaagtag taaaaaagca atagaaatac ttcatgatat
     6421 gggaataaaa gttgcaatgg taactggaga taatgcgaaa actgcaaatg ctatagcaaa
     6481 tcaagtcggt atagatatgg ttttggcaga ggttttacca gaagataagt caaaagaagt
     6541 tgaaaaacta caaaatcaag gaaagtttgt tgcaatggta ggggatggaa taaatgatgc
     6601 cccagctctt gcaaaagcag atatagggat agcaatagga agtggaacgg atgtagccat
     6661 tgaatctgcg gatatagtac ttatgaagag tgatttaatt gatgttccaa cagcaattaa
     6721 acttagtcat gaaactataa aaaatataaa acaaaaccta ttttgggcat ttggatataa
     6781 tacaattggt atacctgtag cagcaggaat attatatgta tttggaggac ctttattaaa
     6841 cccaatgatt gcagcagcag caatgagctt aagttcagtt tcagtcgtat caaatgcact
     6901 tagacttaaa aacttcaagg catataagag agattagatt aataaattaa ataaaatgta
     6961 tccaaaagag ctatctttaa attaaaagga ttaatttaca aattaagata gctctttttt
     7021 catgtaaaat tcacaacaat aagataaaat ataagtaata tacgaaattt taaataaaat
     7081 agaaaggatt gatagaatga attcgtcaat acttgtaatt gaagatgatt caaatataca
     7141 agagttaata tcagaatttt taagtgcaga aggttatcaa gttgatacag caaatgatgg
     7201 gttggaaggt atacaaaagt ttaagcaagg aagttatgat ttagtaatat tagatataat
     7261 gatgccaaac ttagatggtt atggagtttg taaaatgata agaaagtcat caagcgttcc
     7321 aattatattt ttaactgctt taaatgatga aggagaccaa cttaaagggt ttgacttaga
     7381 atgtgatgat tatataacaa aaccattttc atttaatctt cttataaaaa gagtagaagc
     7441 aattttaagg cgaagtaaca agactataaa tgacaaattt atagtttttg aaaaattaaa
     7501 attagattta aacacatata ttgcagagat agatggagaa cctatagaac ttacactaaa
     7561 agagtttaat atattaaaag ctttgataga aaagtatcca caagttataa ctagggaagg
     7621 tcttttggat agtatatggg gttatgacta ttatggagat actaggattg ttgatgcaca
     7681 tataaaaaat ataagaaaaa aaatatcatt accatatatt aaaactgtaa aaggaatagg
     7741 atatacactt gagaaggata tttgataagt gggaaaagtt aagtataaag tataagttat
     7801 tttcaataac gacatcatta ttaatagcat tagctttaat aatatatcta attttatatt
     7861 ttttattgcc atcatattat catgagtata aaattgagtc tcttcaagaa agtttgaaat
     7921 ctttagtaga tagttcaatt catttcgata catatacttt agaagaaaga ctttattata
     7981 tggctaaaga ccaaaatttg gctattttat taaaagataa ccaaggtaaa attgtatatg
     8041 gtaagaatga agtagttatt ttaagatata gtaagtacat gattaatagt cttgaggatg
     8101 agtatagaac ttctatacct atatatacta aagatgcaaa agatggtcca tatactttag
     8161 aactcgttat gccattacaa ccaattgacg aagcaaacga agttataaga aaactaatgc
     8221 catacatcat atcaatagca atattgatag cgattattgg agcatatata tattcaattg
     8281 taataacaaa gcctcttata aacataatag agagtgagag agaacaggaa tatagaagga
     8341 aggattttgt tgctacaata tcacatgaac taaaaactcc aataaccata ataagtggtc
     8401 aaattgaagg tatgatttat agtgtaggta agtataagga tagggatact tatctgaaaa
     8461 aatcgtatga gtgtacacaa gaattaaaag atttagtaaa tgaaatgata gaggtttcaa
     8521 aatcagaaat acttgaaaaa gatttgaaat tggtatcaat aaatataagt gaattattaa
     8581 atagacttgt aaagagacaa gtatttttaa tagaagaaaa acatatgaaa actattttaa
     8641 aaatagaaga aaatttggaa attaaagcag accaagagag aataactaag gcaatcaata
     8701 atataataaa taatgccata aaatattctc ctgaagaatc agagcttata attagactct
     8761 atgataaaaa taaacgaata agtaaaaaga cttctaatca aagagtggtt ttagaaattg
     8821 aaaatactgg tgtaactatc gagaaacgtt atttagaaga aatatttaat ccattttata
     8881 gaatagaaaa atctcgttcg agaaaaactg gaggaagtgg acttggactt tatatagtca
     8941 gccaaatttt taaaagtcat gggtttgatt atagcataaa aaacaaagaa aactctgtag
     9001 tattcacagt agaatttaaa aattaattac ttattataaa gaatatataa ctttcaataa
     9061 tagttcattt taaaatcaca aaaaattcat aatgaaactg tattatataa atataaataa
     9121 gcttaaaacc ataaaataca ccaactataa tgatgagatt tccttacatc ctccatataa
     9181 gtcatcatta tagtaaccca acaattaagt tactatgaat ttaaaaaata acaccgagca
     9241 tataatctat gcttagtgtt atttttattt tgaaataaaa aatatttaat ttattgaaat
     9301 gtatagattt agatagttta aactatattt aagacttaaa aattataatt gatattacaa
     9361 ataaatatat gtactttggg aaaaataaat ataggaagta ttatatttac agaggaggta
     9421 gttttgatgg atagattaaa aagagatatg aaaaatatga aagaagattt aaaagaaggt
     9481 gttaaaaaga cagcttatgt tatgaaaaat acagctgaag atttgaaaga tgatgcacaa
     9541 ggtgctatgg taaacttaga aatgaaaaaa gatgagatga tggaaagata tgaacaaaag
     9601 aaattttcta aagaattaga gaagcaaatg aagaatgaaa tgaaataagc ttttatttat
     9661 gaataacaaa ttattgtatt catataaaaa gtagccagta atattaaata gtttatttaa
     9721 ggaaatagac tttctaaaaa taactgtatt agatttagaa agtctatttc aaattagttt
     9781 tataaataga cataaggata aagtagatgt tatagaacta ga
//
LOCUS       DAMJVK010000126         9312 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.131, whole genome shotgun sequence.
ACCESSION   DAMJVK010000126 DAMJVK010000000
VERSION     DAMJVK010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9312
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.131"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            345..1361
                     /gene="gap"
                     /locus_tag="PC414_003334"
     CDS             345..1361
                     /gene="gap"
                     /locus_tag="PC414_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDF2465705.1"
                     /translation="MKIKVGMNGFGRIGRAVLRIAQEELGDNIEIVAINARATTESLA
                     HLFTYDSCYGTFRGEVEAKDEDTLVVNSKEIKILRYNDPEELPWKELGVDIVIESTGL
                     FTQREKAEKHIKAGAKKVIITAPGKNEDITIVIGVNEKEYDNEKHNIISNASCTTNCL
                     APFAKVLDEKFGIVKGLMTTVHSYTNDQRILDKSHKDLRRARAAAESIIPTTTGAAKA
                     VSRVLPQLEGKLNGFSLRVPTPTVSLVDLVCELKENVTSEQVNSVLKDAAEGELKGVL
                     GYCDKPLVSIDYKGDSRSSIIDALSTMVIEDNMVKVVSWYDNEWGYSSRTVDLVKYVA
                     EKLK"
     gene            1756..2925
                     /locus_tag="PC414_003335"
     CDS             1756..2925
                     /locus_tag="PC414_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465706.1"
                     /translation="MEKLTDRRQVKAVSMETFIFLILLVVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLETVFLIMAMAVLAGALSALLSEFGVIALINKIFAVFMKPLYGLPGASIA
                     GAITTYLSDNPAIIPFAKDKTFTQYFKQYQVPALCNLGTAFGMGLILTTFMISQGTEY
                     VLPALIGNLGAIIGSVISVRIMLTFTKKFYKYSPEEDKATGTLEKKDEFREIREGNVF
                     QRALDAILEGGKMGVDMGMAIIPGVLVVCTLVMLLTFGPSTDPVTGQEVYTGAAYEGI
                     KLLPVIGDKLGFILEPLFGFTSPEAIAFPITSLGAVGAAMSLVPEFIKSGAITPNDIA
                     VFTAMGMCWSGYLSTHIGMMDALNARQLAGKAILSHTIGGLCAGAAAHFIFTLVG"
     gene            3305..7558
                     /locus_tag="PC414_003336"
     CDS             3305..7558
                     /locus_tag="PC414_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HDF2465707.1"
                     /translation="MDELFHIGIDVGSTTVKVVVLNNSNNIVHKEYRRHYSDVKKSVK
                     EVLNGIYEKLGDINTTIIITGSGGIGISKKLGVKFVQEVISSTKSIEYFHPETDVVIE
                     LGGEDAKITYLSGGIDQRMNGICAGGTGAFIDQMASLLKTDASGLNELAKGYNVIYPI
                     ASRCGVFAKTDIQPLINDGANQTDIAMSIFNAVVVQTVSVLSCGRKIEGNVAFLGGPL
                     YFLSELREAFKRVLDLKDENIIFPENAQLYIAIGAGLLSVDEDNIQLKSLIEKLDSIK
                     NIEDGEVNLLEPLFKDKNEYEEFSRRHEKEKINYVDINSIKSNCYLGIDAGSTTTKAA
                     LIDEDGRLVYSYYNSNEGNPLKTTIKVINEVYDILPKNIKILSSTVTGYGEGLIKKAL
                     KIDNGEIETIAHYKAAKFFNKDVDFILDIGGQDMKCLKIKDGVIDNIILNEACSSGCG
                     SFLETFASSLSMTIEEFAHEGIYSQNPVDLGSRCTVFMNSRVKQAQKEGASVGDISAG
                     LSYSVIKNALFKVIKIRDLNEIGNNIVVQGGTFYNDLVLRSFEKLIGKNVIRPNISGI
                     MGAFGSALIAKEKYESGYKTSLLSKEELNNIKLEASVTRCKGCSNHCLLTINKFSDNE
                     IFISGNRCEKGEAIYGDKKIGLEKKKPINLFKYKYNRIFRYKPLEKEEAKNGEIGIPR
                     VLNMYEDYPFWFTFFNAMGFRVVLSDRSSKQLYETGITSIASETVCYPGKLVHGHIEN
                     LIAKGIKNIFYPSVTNENKEDSNADNYYNCPVVISYSEVIKNNVENIRNKNINYINPF
                     ISLNDKEKLKKRLYDELSRHFSDIKITKEEVNHAVDKATDEQNLFKMEIQAAGERALK
                     EIKERNMKGIVLCGRPYHIDPEINHGMPELINSLDMAVLTEDSICHLANVQRPLRVVD
                     QWVYHSRLYKAASFVRDKNYLELVQLNSFGCGLDAVTTDQVQEILNEKSKIYTIIKID
                     EGNNLGAAKIRMRSLKAAMFERESKNIDVKNIKVNRIQYAKNNQITNKHTILAPQMSP
                     IHFQFLEKAVNLSGYNIEILKDTDSSVIEEGLRYVNNDACYPAIIVVGQLITALKSGK
                     YDLNNTSVTITQTGGGCRATNYIGFLRKAIYDAGFKDVPVIALSVNGIEDSGIMDNIS
                     LKLINRLFMSVVYGDLLMKVLYRVRPYEKVTGSANALYEKWVDICKSSLVKAKISVFT
                     RNIKDIVKEFDNLEILDIKKPKVGLVGEILVKFHPIANNNLVDILEREGAEAVVPDLT
                     NFFLSCAFNTIYKHTHLEGSRKSRMIGEAFIYITGIYQRVYKKALDKSERFYAPANIK
                     NVAKSTEPVVSLGNQTGEGWLLTGEMVELLNEGVENIICMQPFGCLPNHIIGKGSIKE
                     LKRLYKNANIIPIDYDPSASEVNQLNRIKLMLSKAFKNI"
     gene            7707..8201
                     /locus_tag="PC414_003337"
     CDS             7707..8201
                     /locus_tag="PC414_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDF2465708.1"
                     /translation="MSLVYDVYKSKLGNLYILSEDGFIVSIYIGDEKFNNLKNECIKK
                     GIDIKRSKEDLKEAISQIDDYFNGKRKVFNLNIRIEGTEFQKSVYREMLKIPYGETLC
                     YSDIARNINNPKSVRAIGQASKSNKIPIIIPCHRVVGKKDIGGYMGNHSDLKEILLNL
                     ERQS"
     gene            8317..8694
                     /locus_tag="PC414_003338"
     CDS             8317..8694
                     /locus_tag="PC414_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HDF2465709.1"
                     /translation="MCDKVIASKYHEKGFNCAESVIKAYNEEFNTDIPVCLGSGLGSG
                     CGVASLCGAVNASNIIIGYVKGRNHEDESTKAKVYAKDLTTTVRKEYGSELCIDLKKD
                     LVACREIMDFAYDSLKETLKKEL"
     gene            8900..>9312
                     /locus_tag="PC414_003339"
     CDS             8900..>9312
                     /locus_tag="PC414_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufBD protein"
                     /protein_id="HDF2465710.1"
                     /translation="MKNLNLLINKLYSKNHNEAYKTFLFLENESLKSNITYCFFDSFL
                     EMIDNENSYIRTRGLLLISANAQWDIDNKIEINIDSILSHIVDKKPSVSRMFIKSIPN
                     ITKYKENLINRIKMELSNADISIYNNNMKPLVEKD"
BASE COUNT         3774 a          962 c         1668 g         2908 t
ORIGIN      
        1 tatatttttt gaactaaata aaaatttatt tttattattt taaattagag gagattcttg
       61 aagatatatt atttttaaaa ataagtatag tttaaaaatt caaaaaaaag atggcaaaat
      121 atgtcccaag ggacattgag taaaataaat gatttcagct tatttacact aaaaagtaca
      181 tactaaatga ggggtaaaat aaagaaaaat gatatactaa ttgtcttttg ataataaaaa
      241 aacatggaat ttgattattt aatcaagaaa aagtattgaa caaatgattt atttagtgta
      301 caataatcat gtaaaaacat agtaatggag ggagacattt cataatgaaa attaaggtag
      361 gaatgaacgg atttggaaga ataggaagag ctgtattaag aatagctcaa gaagaattag
      421 gagataatat agaaattgta gccataaatg caagagcaac aacagaatct ttggcacatt
      481 tatttacata tgattcatgc tatggaactt ttagaggtga agtagaggca aaggatgaag
      541 acactctagt agtaaactct aaagagataa aaatattaag atataatgac ccagaagaat
      601 taccatggaa agaactgggg gttgatatag taatagaatc aacaggatta tttacacaaa
      661 gagaaaaggc agaaaagcat attaaagcag gagcaaaaaa agtaattata acagctccag
      721 gaaaaaatga agatataact atagttatcg gagttaatga gaaagaatat gataatgaaa
      781 agcacaatat aatttcaaat gcatcttgta ctacaaattg tctagctcct tttgctaaag
      841 tattggacga aaaatttggt atagtaaaag ggttaatgac tacagtacat tcatatacta
      901 acgatcaaag aatattagat aaaagccata aagatttgag aagagcaaga gcagcggctg
      961 aatctataat acctaccact acaggagcag caaaagctgt gtcaagagta ctaccacaat
     1021 tagaaggaaa attaaatggt ttttcactta gagtaccaac accaacagtt tcacttgttg
     1081 atttagtgtg cgaacttaaa gaaaacgtta catctgagca agttaactct gtgttaaaag
     1141 atgctgctga aggagaatta aaaggtgttt taggatattg tgacaagcca cttgtttcta
     1201 tagattataa aggagattca agatcatcta ttatagacgc tctatccaca atggttatag
     1261 aagacaatat ggttaaggta gtttcttggt atgataatga atggggatat tcttcaagaa
     1321 ctgttgactt agtaaaatat gtagctgaaa aattaaaata atatatattt cttaaagcaa
     1381 attttaaaaa aattttttaa aaaaattata taaaactaaa aaaaaagatt atgaaaaata
     1441 ttgaaggaaa aattttttca taatcttttt tctttcatgc aaaaagatac aagttacaac
     1501 ttatatttgc atattaggtc gttagagaag tatatatata tttagtaagg aatcatactt
     1561 tttataaaat gaaagataag atgtcgaaaa cttgcataaa tctgaaaaat ttttattatt
     1621 aaaatactga tttccatttt aaaaatgata aaaaaatgac aaataaagtt gcaaatagta
     1681 atacttataa gtataattaa aaaagagaaa aggttttgtt aaactttata acaaaaaaag
     1741 aagggatgat tcaaaatgga gaagttaaca gacaggagac aggttaaggc agtaagcatg
     1801 gaaaccttca ttttcctaat attactagta gttggttttg ggtatgttgg tagtattatg
     1861 ggagcaggaa tgatgttcaa agtaataatg agtacagcac atgctttact attggagaca
     1921 gttttcttaa ttatggctat ggcagttctt gcaggcgcat taagcgcact actttcagag
     1981 tttggagtaa tagcgttaat taataaaatt ttcgctgtat ttatgaagcc tttatatgga
     2041 ctacctggtg ctagtatagc aggagctata acaacttatt tatcagataa tccagctatt
     2101 ataccttttg ctaaagataa aacattcaca cagtatttta aacaatacca agttccagca
     2161 ttatgtaact taggaacagc ctttggtatg ggacttatct taacaacatt tatgatttct
     2221 caaggtacag aatatgtact tccagcatta attggtaatt taggtgcaat tataggtagt
     2281 gtcataagtg ttagaataat gttgacattt actaagaagt tttataaata cagtccggaa
     2341 gaagataaag caacaggaac attagagaaa aaggatgaat ttagagaaat cagagaagga
     2401 aatgtatttc aaagagcttt agatgcaatt cttgaaggtg gtaaaatggg ggtagatatg
     2461 ggaatggcta taattcctgg agtcctagta gtatgtacat tggtgatgtt attaactttt
     2521 ggaccatcta cagaccctgt tacaggtcaa gaagtttata caggagctgc atatgaagga
     2581 ataaaattac ttccagttat tggagataaa cttggattta tattagaacc attatttgga
     2641 tttacatcac cagaggcaat agcattccct ataacttcat tgggagctgt tggcgcagca
     2701 atgtctctag ttccagagtt tataaagtca ggagctataa ctcctaatga tatagcagta
     2761 tttacagcta tgggtatgtg ttggagtgga tatttaagta cacatattgg tatgatggat
     2821 gcattaaatg ctagacagtt ggcaggtaaa gctattcttt ctcatacaat aggtggttta
     2881 tgtgcaggag cagctgctca ctttatattt acactcgtag gataaatgta atcagctaag
     2941 ataaaaaata atatacaaaa aatggttgtt gatatgtaat aagaatttac atatcaacaa
     3001 ccattttttt ctgactaaag ataatttata atacataaac attaagtgga tattagagtt
     3061 aagttatatt tatgtaaaaa gtaacaaaaa tctgtagaaa tttatgacaa aatttagtac
     3121 actaactata tgttcttatt taaaatttaa ggttagaatt aggaattacc tagtagagta
     3181 agctttgatt aataaaagaa tcataaaata aaaatattag tcataaagat tataaataac
     3241 ttacttaaaa taaagccata gaacttacaa aaattaaaaa agcatataaa tatggagggg
     3301 tacaatggat gaattatttc atataggaat tgatgtaggt tctacaacgg tgaaggttgt
     3361 ggtacttaat aatagtaata atatagttca taaagaatat agaagacatt attctgatgt
     3421 aaaaaaatca gttaaagaag tactaaatgg tatatatgaa aaattgggag atataaatac
     3481 taccattata ataactggtt caggtggaat tggaatatca aaaaaattag gtgtaaaatt
     3541 tgttcaggaa gttatatcta gtacaaaatc aatagagtat tttcatcctg agactgatgt
     3601 tgtcatagaa cttgggggtg aagatgcaaa gataacatat ctaagtggag gaatagacca
     3661 aagaatgaat ggaatatgtg caggtggcac tggagcattc atagaccaga tggcatcact
     3721 cttaaagaca gatgccagtg gattaaatga acttgctaaa gggtataatg ttatatatcc
     3781 catagcttca agatgtggtg tttttgctaa aacagatatt caacctctta taaatgatgg
     3841 agccaatcag actgatatag caatgagtat tttcaatgca gttgtcgtac aaactgtgag
     3901 tgtcctttct tgtggaagaa aaatagaagg aaatgttgcg tttttgggtg gtccattgta
     3961 ctttttatct gaacttagag aagcatttaa aagagtatta gatttaaaag atgaaaacat
     4021 aatcttccca gaaaatgcac aactttatat tgctattgga gcaggattat tatcagtaga
     4081 tgaagataat atacaactaa aatctctaat agaaaaattg gatagtataa agaatattga
     4141 agatggagaa gtaaatttat tagaaccttt atttaaagat aaaaatgagt atgaagaatt
     4201 ttcaagaaga catgaaaaag aaaaaataaa ttatgtggac ataaattcaa taaaaagcaa
     4261 ttgttattta ggaattgatg caggctcaac tacaactaaa gcagcactta ttgatgagga
     4321 tggaagactt gtatattctt attataatag caatgaagga aatccattaa aaacaaccat
     4381 taaagttata aatgaagtat atgatatatt accaaaaaat attaaaatat tgagttcaac
     4441 tgtaactgga tatggagaag gtcttattaa aaaagctctt aaaatcgata atggagagat
     4501 agagactata gcacattaca aagctgctaa atttttcaac aaagatgtag attttatact
     4561 tgatattggt ggtcaagata tgaagtgcct aaaaataaaa gatggagtaa ttgataatat
     4621 aatattaaat gaggcttgtt catcaggttg tggctcattt ttagagacat ttgcaagttc
     4681 tttatctatg acaatagaag aatttgcgca tgaaggtatt tattctcaaa atcctgtaga
     4741 tttaggttca agatgtactg tatttatgaa ttctagagta aaacaagctc aaaaagaagg
     4801 tgcaagtgtt ggagatatat cagcagggct atcttattca gtaataaaaa atgctttatt
     4861 taaagtaata aagattagag atttaaatga gattggtaat aatattgttg tacaaggtgg
     4921 aaccttttat aatgatttgg tgcttagaag ctttgaaaaa ctaattggaa aaaatgtaat
     4981 tagacctaat atttcaggaa ttatgggagc ttttggttca gcacttatag ctaaagaaaa
     5041 atatgaaagt ggatacaaaa catccttgtt atctaaggaa gagttgaata atatcaagct
     5101 agaagcaagt gtaactagat gtaaaggttg ttcaaatcat tgtcttctta caataaataa
     5161 attttctgat aatgaaattt ttatttcagg taatagatgt gagaagggtg aagctattta
     5221 tggtgataaa aaaataggat tagaaaagaa aaaaccaata aatttattta agtacaaata
     5281 caatagaatt tttagataca aaccattgga aaaagaagaa gcaaaaaatg gggaaatagg
     5341 gataccaaga gtattaaata tgtatgaaga ttatccattt tggtttactt ttttcaatgc
     5401 tatgggcttt agagttgtac tttcagatag gtcatcaaaa cagttatatg aaactggtat
     5461 aactagtatt gcatctgaaa cagtttgtta tccaggaaaa ttagttcatg gacatataga
     5521 aaatttaatt gcaaaaggta taaaaaatat attttatcca agtgtaacaa atgaaaataa
     5581 agaggattca aatgcagata attattataa ttgtcctgtt gtaatatcat attctgaagt
     5641 aataaaaaac aatgtagaga atataagaaa taagaacata aattatataa atccatttat
     5701 aagtctaaat gataaggaaa aattgaagaa aagactctat gatgaattat caagacattt
     5761 ttctgatata aaaataacta aagaagaagt taatcatgca gttgataaag ctactgatga
     5821 acagaattta tttaaaatgg agatacaagc agcaggtgaa agagcactta aagaaataaa
     5881 agaaagaaat atgaagggga ttgtgttatg tggaagacca tatcatatag acccagaaat
     5941 aaatcatgga atgccagagc ttataaattc attagatatg gcagtgctta ctgaagactc
     6001 tatatgtcat ttagccaatg tacaaagacc acttagagtt gtagaccaat gggtatatca
     6061 ttcaagattg tataaagcag ctagttttgt aagagataaa aactatttgg aacttgttca
     6121 acttaattca tttggctgtg gattagatgc agtaactaca gaccaggttc aagaaatact
     6181 taatgaaaaa tcgaagatat atactattat aaagattgac gaaggcaaca atttaggagc
     6241 agctaagata aggatgaggt ccttaaaagc agctatgttt gagagagaaa gcaaaaatat
     6301 agatgtaaaa aatataaagg taaacaggat acagtatgct aaaaataatc agataacgaa
     6361 caaacatact atattagcac cacaaatgtc tccaatacat ttccaattcc ttgaaaaagc
     6421 tgttaactta agtggttata atatagaaat tctaaaagat actgatagta gtgtaattga
     6481 agaagggtta agatatgtaa ataatgatgc atgttatcca gctataatag ttgtaggtca
     6541 gctaataaca gcattaaaat ctggcaaata tgatttaaat aacacttctg ttactataac
     6601 tcagactggt ggaggttgta gagctacaaa ttatattgga ttcttaagaa aggctatata
     6661 cgatgctgga tttaaagatg ttcctgtcat tgcacttagt gtaaatggaa tagaagatag
     6721 tggtataatg gataatatat cactaaaatt aatcaataga ctctttatgt ctgttgttta
     6781 tggtgatttg ctgatgaaag tactatatag agttagacca tatgaaaaag ttactggaag
     6841 tgcaaatgct ctatatgaaa aatgggttga tatatgcaaa tcttctcttg ttaaagcaaa
     6901 aatttctgta tttactagaa atataaaaga tatagtaaaa gaatttgata atttagagat
     6961 actagatatc aaaaaaccaa aagtaggttt ggttggagaa atattggtga aattccatcc
     7021 aattgcaaat aataatttag tagatatatt agagcgagaa ggtgctgaag cagtcgtgcc
     7081 agaccttaca aatttcttct taagttgtgc ctttaatact atatacaaac acactcattt
     7141 agaaggaagt agaaagagta gaatgatagg agaggcattt atatacataa ctggaatata
     7201 tcaaagagta tataaaaaag cacttgataa gagtgagaga ttttatgctc cagccaatat
     7261 aaaaaatgtt gctaaaagta cagaaccagt agtgtcatta ggtaatcaaa ctggagaagg
     7321 ttggctttta acaggtgaaa tggttgaatt attaaatgaa ggtgtagaaa acattatttg
     7381 tatgcaacct tttggatgtt tacctaatca cataattggt aagggttcta taaaagaatt
     7441 aaagaggttg tataaaaatg caaatataat acctattgat tatgaccctt ctgcaagtga
     7501 ggtaaatcaa cttaacagga taaaacttat gctttctaag gcttttaaaa atatataaga
     7561 tttatatttt gatatgttaa aagttaatga atttaaaaat atactctata ttaagtatct
     7621 ataaaaaaga gttatcctat tttttagggt aactctttat tagttaaaaa attatatgta
     7681 tactgatatt aaagggggga agtaatatga gtttagtata tgatgtttat aaatcaaaat
     7741 taggaaattt gtacatatta agtgaagatg gttttatagt tagtatatac ataggagatg
     7801 aaaaatttaa taatttaaaa aatgaatgta ttaaaaaagg aattgatata aagagaagta
     7861 aggaagattt aaaggaagca ataagccaga tagatgatta ctttaatgga aaaagaaaag
     7921 tctttaattt aaatattcgt attgaaggga ctgaatttca aaaatcagtt tatagggaaa
     7981 tgttaaaaat accttatgga gaaacattgt gttattcaga tatagcaaga aatataaaca
     8041 atccaaaatc agtaagagca ataggacaag caagtaaaag taataaaatt ccaataataa
     8101 taccttgtca tagagtagtt ggcaaaaaag atattggtgg atatatgggg aatcattctg
     8161 atttgaaaga gatactatta aatttagaaa gacagagtta acttagaaag aaatgttttt
     8221 attgttaata tttgatataa taatagtaaa aagaagtatt ttttacaaaa acaatgaaga
     8281 atttatttta atttacaaaa atgaggggga attttaatgt gtgataaagt tatagcatca
     8341 aaatatcatg agaagggatt taattgtgca gaatcagtta taaaagcata taatgaggaa
     8401 tttaatacag atataccagt ttgtttagga agtggactgg gttcagggtg tggggttgct
     8461 agtttatgtg gtgcagtaaa tgcatcaaat ataataattg gatatgtaaa aggcagaaac
     8521 cacgaagatg aaagtactaa agctaaagta tatgctaaag atttaactac aacagtaaga
     8581 aaagagtatg gttcagagtt atgtatagac ctaaaaaaag atttagttgc ttgtagagaa
     8641 ataatggatt tcgcatatga tagtctaaag gaaactttaa aaaaagaatt ataagaaaat
     8701 aagctatcaa atataaatta tttgatagct tatttttata gccgatacta aataaatcaa
     8761 atatatttta gaattattat ttgtaaataa acagcaaata attttaggct ttttatttaa
     8821 tatagttttt atttatattt atttttgaat atgttatact ttgtttaaat aaatatttgt
     8881 aatgagggag atgagaagat tgaagaatct caatttactt attaataaat tatattcaaa
     8941 aaatcataat gaagcatata aaactttttt attcttagaa aatgaaagtt taaaatcaaa
     9001 tattacatat tgtttttttg actcattcct agaaatgatt gataacgaaa actcatatat
     9061 tcgaacaagg ggattgttat taatctctgc aaatgctcag tgggatattg ataataaaat
     9121 tgaaataaat atagatagta tactaagtca tatcgtagat aaaaaacctt ctgtatcaag
     9181 aatgtttatt aaatccatac ctaatattac aaagtataaa gaaaatctta ttaatcgcat
     9241 aaaaatggaa ttaagtaatg cagatataag tatttataat aataatatga aaccattagt
     9301 agaaaaggat at
//
LOCUS       DAMJVK010000127         9037 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.132, whole genome shotgun sequence.
ACCESSION   DAMJVK010000127 DAMJVK010000000
VERSION     DAMJVK010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9037
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.132"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(81..689)
                     /locus_tag="PC414_003340"
     CDS             complement(81..689)
                     /locus_tag="PC414_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HDF2465711.1"
                     /translation="MEKDFFRKNGIDLAKSILGKYLIRKYENKVIVTKIIETEAYMGV
                     NDKGAHVYGNKKTDRTKPLYLDGGHIYVYLIYGMYNCLNLSANIENVPECVLIRGVEP
                     ITSLDEISMNRYNKAYTELSKYQVKNITNGPGKLCKALKIDRSLNSKSIMGEELYISD
                     FYYDDKGKKVFSKDELDIKTSKRINIDYAEEAKDFLWRFYIE"
     gene            complement(798..3536)
                     /locus_tag="PC414_003341"
     CDS             complement(798..3536)
                     /locus_tag="PC414_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HDF2465712.1"
                     /translation="MESKGTLSKNKVYILANGLFLVIALLNINLYTVSINMYRIILLW
                     IFGYSIVYISSFKTDEFINILKVGCLVLVFVSILGVVNLKIQKFQIVLLNLSIINMST
                     FIYLTISKVKKLRISPILIFYSAFVILDYFIRNIFSDINWNMIIILINICISMFNLLY
                     LLNKLDSNIEHYKLIKDLTYFVFFTTIICFVAISKINIVDSDRLIGVIYLLLCNHTYY
                     VYVYSLNNRVVYPYYELDSINKKLSKKSEQLGKINLAIEKDMIIQRTLKNYIDQRKEL
                     LRQALDTIPNVWIVTDYDFNISYTNNKFKDEFSKDMNNYYKILTFIKEDEKVAEKLKK
                     FDNDISIIDKLVELNDKIYLLSLSNNKDESNYLISLNDITNEIKIDEEIRNINKDYEN
                     IILNIPSPILVRSAEGGIKKNKVISINKKYEKSFKCVSSDLVDMTLEQYFETFNIDFF
                     DNREFKRLNLTQEKKAEIVSYNDTANCIVNFVMSDSEGEEHVEEVRVGDYWSDNKLFK
                     LLTFRDITKEINILRTVNEQKIIYEKLLDVIPEAIFLEDLSTSRVLYTNRAFRELFGI
                     SSDVLGVTTQKYRNILVKKYINNLNIGEREKSIHIVNENNHIKEVKMISRTLYFGQKR
                     SRVRIIKDLSVQRESERLKKALIKQRQYDQMKMEFYANISHELKTPLNNIYSSVQLIE
                     NLYKKGKIIDFQDILKEHIKITKQNMFRLLRLIDNIINISQVKSDIYKIKAVNFDIID
                     ITERIVTSISSYAKSKGIDLIFDTDEETVMVGLDPESIERIILNILSNAIKFTLEGGE
                     ILVGIYKKDETVEIIIKDTGVGIDKEKLKDIFNRFKQIENSGISNEFGSGIGLCLTKS
                     LIEIQNGKIYIDSKVGEGTNVKVIFPIKEVVEEVYDNSNYNDNIEKFEIEFFDIYK"
     gene            complement(3684..4475)
                     /locus_tag="PC414_003342"
     CDS             complement(3684..4475)
                     /locus_tag="PC414_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2465713.1"
                     /translation="MKLKKLLSVALVSAIAISAVGCSNKEDKKILVGASSNPHAKILE
                     VAKPLLKEKGYDLEVKIFDDYVLPNTALDEGSLDANFFQHIPFLEETVKEKGYKLTYT
                     SKVHIEPMGFYSEKVKSLDELKDGAVIAVPNDATNGARALKLLAKNKLIEVKDGELIT
                     KKDITKNPKNIVIKEMNAEQLPTVLKDVDGAVINSNYALTANLNPTKDAIVIESSDSP
                     YVNIIACRENNKDSDKIKALSEAMNSKEVKKFIQDEYKGSIVPAF"
     gene            complement(4511..5167)
                     /locus_tag="PC414_003343"
     CDS             complement(4511..5167)
                     /locus_tag="PC414_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2465714.1"
                     /translation="MNSLIDFLTTLFPNALLQTLYMVIVPTIVATILGFILAIILVVT
                     KPDGLKPNSTINSALGFIVNIFRSFPFMILIVAMIPITRLIVGTSIGETAAIVPITIG
                     AAPFIARIIESSLNEVDKGLIEAAKSFGATKRQIVFKVMIKEAMPSIVSGITLSIISI
                     LGYTAMAGAVGAGGLGNIALIYGYQRFDTAVMVYTVIALIILVQIIQGVGNLAYKKLK
                     "
     gene            complement(5160..6125)
                     /locus_tag="PC414_003344"
     CDS             complement(5160..6125)
                     /locus_tag="PC414_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HDF2465715.1"
                     /translation="MISIKNVNKYYGKIQVLKDVSIEIESGEIFGIIGHSGAGKSTLL
                     RCINGLEEYQEGSVLVSDKEVKSLNEKQMRDLRKELGMIFQHFSLLERKTVFDNVALP
                     LECFGYSKAEIKKRVLELLEVVGISEKKNDKPRNLSGGQKQRVAIARALALNPQVLLC
                     DEATSALDPNTTKSILSLLEDINKKLGITIIVVTHQMEVIKQICGRVAIMENGEVLEV
                     GDTEEIFLRNTKGLRKLIGEESIILPKGTNIKILFPKDISNEAIITTMARELNIDVSI
                     IFGKLEQFKDDILGSLIINISDKSGEQVKQYLTSKGIRWEEMINE"
     regulatory      complement(6231..6337)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(6627..7571)
                     /locus_tag="PC414_003345"
     CDS             complement(6627..7571)
                     /locus_tag="PC414_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HDF2465716.1"
                     /translation="MSRAERLIELMITINAKRSFTAGELAEEFSVSKRTILRDLQVLE
                     SIGFPLYSKVGAAGGYHVLKERILPPIAFSESEVKSIFFAYQSLEYYNDLPFEQETIS
                     VLKKFLNCIPNDIQYNIENIRRKFVFWTPDRHCSTPLLKELFNIVMNEFTIKIEYSSK
                     QKNSVRTIVPIGLYAMNGLWYCPAYCIKSESIKEFRVDRIVKILSIENLSSKKYKVLS
                     SIHDYLKNMEVGTDYHIKINLTDEGVKRCETEFLLARGLKILSKGGYIDMYIPKSTLN
                     WVAEYFLTFGKNATIIEPIELKHLIKSKVLELYNHHCI"
     gene            7682..8149
                     /locus_tag="PC414_003346"
     CDS             7682..8149
                     /locus_tag="PC414_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465717.1"
                     /translation="MENDTIKADDILNYCLSNLDDVVLMDSWGERAIYYNPNGVLKRG
                     VYVLTIKEKDSNNDKGSLVSRPNVYRVNIGLKKETFIEMFGYIPKRPGVGQIVDMDFD
                     FTKLDTIMPHPIYSWMGWICVLSPTEKTFENFKTLIGESYNFAKQKFKKRKNK"
     gene            complement(8319..8918)
                     /locus_tag="PC414_003347"
     CDS             complement(8319..8918)
                     /locus_tag="PC414_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465718.1"
                     /translation="MKLKRTLFVGLALVLAIGITACTKNNEKPSENTGKNPNESVNNN
                     EGYMNYYSTSYNDYIAGLNRYSIYDTPESINNQFKDKEYPGNEKYLSDVKAAYKDSRD
                     KIQSFVTSLKKDGKTEDTELKKMNDDLIAEGERLVKDIDKRIEKLDKVSDEDMKKGQN
                     DFIKLVHGTEDVGNDIGSGFRKMIKDMNDRLGITGNNKK"
BASE COUNT         2933 a         1493 c          876 g         3735 t
ORIGIN      
        1 aactaaaaat tctataaaaa aataagatac atacctataa aataccttcc tatagataca
       61 tatcttacct atataaaaac ttattctata taaaaacgcc ataaaaaatc ttttgcttct
      121 tctgcataat ctatatttat acgtttacta gtttttatat ctaactcatc tttactaaat
      181 actttttttc ctttatcatc ataataaaaa tcacttatat ataattcttc acccattata
      241 ctcttactat ttagacttct atctattttt aaagctttgc aaagttttcc tggtccgtta
      301 gtaatatttt ttacttgata tttactcaac tcagtgtaag ctttgttgta tctattcata
      361 gatatctcat ctaaagaagt tataggctct acccctctta ttaatacaca ctctggcaca
      421 ttttctatat ttgcagataa attcaagcaa ttatacatac cataaattaa ataaacatat
      481 atatgtccac catctaaata taatggctta gttctatctg tctttttatt gccatataca
      541 tgtgctcctt tatcatttac acccatgtat gcttctgtct ctatgatttt ggtaacaatt
      601 actttatttt catatttcct tattagatat ttacctaata ttgatttggc taaatcaatt
      661 ccattttttc taaaaaaatc cttttccaaa atttcacctc ataaaattta tttaaaacgc
      721 atcaaataca actaaagtaa taaataaaat taactcttat taatgtgata tatataaaca
      781 ataaatttag acatttatta tttatatata tcaaaaaatt ctatctcaaa cttttcaata
      841 ttatcattgt aatttgaatt atcatatact tcttcaacaa cctctttaat tgggaatata
      901 acttttacat tagttccttc tcctacctta ctatctatgt aaatcttccc attttgaatt
      961 tcaatcaatg attttgtaag gcataaacct ataccacttc caaattcatt tgatatacca
     1021 ctgttttcaa tttgtttaaa tcgattaaat atatccttta atttttcttt gtctatgccc
     1081 actccagtat cttttataat tatctctact gtttcgtctt ttttatatat acctacaagg
     1141 atttctcctc cttctaaagt aaattttata gcatttgata atatatttag tattattctt
     1201 tctatcgatt ctgggtcaag ccctaccata acagtttctt catctgtatc aaaaattaaa
     1261 tctattcctt tagacttagc ataactacta atagatgtaa ctattctttc tgttatgtca
     1321 ataatatcaa aattaactgc tttaatctta tatatgtctg acttaacttg agaaatattt
     1381 attatattat ctattaatct caataatcta aacatatttt gtttagttat ttttatatgt
     1441 tcttttagta tatcctgaaa atcaattatt tttccttttt tataaaggtt ttcaataagt
     1501 tgaactgaac tatatatatt atttaaaggt gtcttaagtt catgagatat attagcataa
     1561 aactccatct tcatttggtc atactgtctt tgttttatta atgctttttt aagtctttca
     1621 gactctcttt gcacacttaa atctttaata attctcactc tacttctttt ctgtccaaag
     1681 tataatgttc ttgatatcat ttttacttct ttaatgtgat tattttcatt tactatatgg
     1741 atactcttct ctctttctcc tatatttaaa ttgtttatgt atttttttac taatatattt
     1801 ctatattttt gtgttgtaac tcctaataca tcactagaaa ttccgaacaa ctctctaaag
     1861 gctctatttg tatatagtac tcttgatgta cttaaatcct caagaaaaat agcctctggt
     1921 ataacatcca gtaatttttc atatattatt ttttgttcat ttactgttct aagaatattt
     1981 atctctttag ttatatctct aaatgtaagc aatttaaaga gtttattatc tgaccaatag
     2041 tctccaactc taacttcttc aacatgttct tcaccttctg agtcagacat cacaaaatta
     2101 actatacagt ttgctgtgtc attgtaactt actatttcag ctttcttttc ttgtgttaga
     2161 tttaatcttt taaattccct attgtcaaag aaatctatgt taaaagtttc aaaatattgt
     2221 tctaatgtca tatcgactaa atcacttgaa acacatttaa aactcttttc atatttttta
     2281 tttatactta ttaccttatt cttctttatg ccaccttcag cacttcttac aagtatagga
     2341 gatggaatat ttagtataat gttttcatag tctttattta tatttcttat ttcttcatct
     2401 attttgattt catttgtaat atcatttaaa cttattaagt aattgctttc gtctttatta
     2461 ttacttaaac ttaacaaata aattttatca tttaactcta ccagtttatc aattatactt
     2521 atatcgttat caaatttctt caatttttca gcaacttttt catcttcctt tataaaagtt
     2581 aatattttat aataattatt catatccttt gaaaattcat ctttaaattt attatttgtg
     2641 taacttatat taaaatcata gtctgttact atccatacat ttggtattgt atccagagct
     2701 tgtctaagta attcttttct ctggtctatg tagtttttta atgttctttg tataatcata
     2761 tccttttcta ttgctaaatt tatctttcct aattgctctg actttttact taatttttta
     2821 ttgattgaat caagttcata atatggatac actactctat tattaagact gtatacatat
     2881 acataataag tatggttaca taatagtaaa tatattactc ctattaatct atctgaatct
     2941 actatattaa tcttagaaat tgctacaaaa catataatag ttgtaaaaaa aacaaaatat
     3001 gttaaatctt ttattaactt atagtgctca atgttactat caagtttatt caacaaatat
     3061 agtaaattaa acatagatat acaaatatta attaatataa taatcatatt ccagttaata
     3121 tcactaaaaa tatttcttat aaagtaatct aatatcacaa aagcactata aaaaattagt
     3181 attgggctta ttcttagttt ttttacttta gaaatggtta agtatataaa agtagacata
     3241 ttaattatgc ttaagttaag caatacaatt tggaattttt gaatttttaa atttactact
     3301 cctaaaatac ttacaaaaac taatacaaga caccctactt tcaatatatt tataaattca
     3361 tctgttttaa agctacttat ataaactata gaatatccaa atatccaaag taaaattatt
     3421 ctatacatat ttatagaaac tgtatacaaa tttatattta aaagtgcaat aaccaaaaat
     3481 aacccattag ctagtatata gaccttattc ttacttaaag ttcctttaga ttccatattc
     3541 cccaccactt cttcattttt atatagtttt acctataaat atgttaccac aattcttcca
     3601 aaaaatttgt cactttctag ttataaaaaa aaatgagtaa atatttttaa ttttactcat
     3661 tttgtagatt ttgtatattc agtttaaaaa gctggaacta tacttccttt atattcatct
     3721 tgtataaatt ttttaacttc tttactattc atagcctctg ataaagcctt aatcttatca
     3781 ctatctttat tattttctct acaagctatt atatttacat atggagaatc acttgattct
     3841 attactattg catccttagt aggatttaag ttagctgtta aagcataatt agaatttatt
     3901 actgctccgt caacatcttt taatactgtt ggtaattgct ctgcattcat ttcttttatt
     3961 actatatttt taggattttt agttatatct tttttagtta taagctctcc atcttttact
     4021 tctatcaatt tattttttgc taataattta agtgctctag ctccattagt tgcatcattt
     4081 ggcacagcta taacagctcc atcttttaat tcatctaatg acttaacttt ttctgaatag
     4141 aatcccattg gctctatatg aactttagat gtataagtaa gtttatatcc tttctccttc
     4201 acagtttcct ctaaaaatgg aatatgttga aagaagttag catccaaaga cccttcatct
     4261 aaagctgtat ttggaagtac ataatcatca aatattttta cttctaaatc atatcctttt
     4321 tcttttaaca aaggttttgc aacttccaat atctttgcat gtggatttga tgaagctcct
     4381 actagtattt ttttatcctc tttgtttgaa caacctactg ctgatattgc tattgctgac
     4441 accaatgcta ctgataataa tttctttaat ttcattttta ctccccctct ttaaaatgtt
     4501 tataattaat ttattttagt ttcttataag ccagatttcc tactccctgg attatctgaa
     4561 ctaatattat aagtgctatt acagtatata ccattactgc tgtatcaaat ctttgatatc
     4621 cataaataag tgctatatta cctaatcctc cagctcctac tgcacctgcc atagctgtat
     4681 aaccaagtat agatattatt gataatgtaa ttccagatac tattgaaggc atagcttcct
     4741 ttatcataac tttaaatact atttgtcttt ttgtagctcc aaaagatttt gctgcttcta
     4801 tcaatccttt atcaacttca tttaatgagc tttctataat tctagcgata aacggtgctg
     4861 ccccaatagt tattggcact attgcagcag tttcccctat acttgttcca acgattaatc
     4921 tagttattgg aatcattgca actataagta tcataaatgg gaaacttcta aatatattta
     4981 ctataaatcc taatgcacta tttattgtgc tatttggttt taatccatct ggttttgtaa
     5041 ctactaatat gattgctaat ataaatccta atattgttgc cactatagtt ggtactataa
     5101 ccatatacaa agtttgtaac aatgcatttg gaaatagtgt tgttaaaaag tcaattaaac
     5161 tattcattta tcatttcctc ccatcttatg cccttactag ttagatattg ttttacttgc
     5221 tctccacttt tatctgaaat gtttataata agtgaaccta aaatatcatc tttaaattgt
     5281 tctaatttcc caaatattat agacacatct atatttagtt ctcttgccat agtcgtaata
     5341 atagcttcat tagaaatatc tttagggaat agtatcttta tatttgttcc ttttggcaat
     5401 attatacttt cttcacctat taattttcta agtcctttag tatttcttaa gaatatttct
     5461 tctgtatctc ctacctctaa tacttctcca ttttccatta ttgctactct tccacaaatc
     5521 tgctttataa cttccatttg atgagttaca actattattg ttattcccaa ttttttattt
     5581 atatcttcaa gcaatgaaag aattgacttt gtagtatttg ggtctaatgc tgaagtcgct
     5641 tcatcacaaa gaagtacttg tggatttagt gctaaagctc tagcaattgc aaccctttgt
     5701 ttttgtccgc cacttagatt tctgggttta tcatttttct tttcagatat tccaactact
     5761 tctaataact ctaaaactct ttttttaatt tctgcctttg agtatccaaa acattctaat
     5821 ggcaatgcta cattatcaaa tacagttttt ctttcaagca atgaaaagtg ttgaaaaatc
     5881 attcctaact cttttcttaa atctctcatt tgcttttcat tcaatgactt tacttcttta
     5941 tcagaaacaa gtacactgcc ttcttggtat tcttctaatc cgtttataca tctcagtagt
     6001 gttgatttcc ctgcaccact atgacctatt attccaaaaa tttctccaga ttctatttca
     6061 atacttacat ctttaagcac ttgaatcttt ccataatact tattaacatt ttttatgcta
     6121 atcataaaat ttcctcccat atattctgct cagtttttat gtttatcctc tcttatgtat
     6181 tacaacaaaa taaaagcccg agtataaact caggcttaga aagctattta tactcatcta
     6241 tcaactatat tagttgcagg atttagcacc aatgtatata aaatatactg gttgccgggt
     6301 ttcaaagggc cagtccctcc accactcttg ataagacgtt atttaattta attctattat
     6361 catttgttgc tatgttatat atgatatatt cattaatagt atttgtcaat aaatttattt
     6421 aaatttttta ttttttatta tcgttaatta actattttta attagttttc tagctcctaa
     6481 cactttatat tttaatgtct atataactat agaacatcat tataagaaat aaattaaata
     6541 acatcaatct atctaaatca tctgtaaagt aaattaagaa aattaacatt ttatccaaac
     6601 tctatcctac tattaaataa aataatttaa atacaatgat gattatatag ctctagtact
     6661 ttagacttta tcaaatgttt aagttctata ggttctatta ttgtagcatt tttcccaaaa
     6721 gttaaaaaat attcagccac ccagttaagt gtagattttg gtatatacat atctatgtat
     6781 ccacctttag acaagatttt caaccctctt gccaacaaaa attctgtttc acaacgtttt
     6841 actccctcat ctgtcaaatt aattttaata tgataatctg tacctacttc catattctta
     6901 agataatcat gtatagaaga taaaacctta tatttcttac ttgataagtt ttctatactc
     6961 aaaatcttca caattctgtc tacacgaaat tccttaatag attctgattt tatacagtaa
     7021 gctggacaat accaaagtcc attcatggca tacaaaccaa taggaacaat ggtacgtaca
     7081 ctattttttt gctttgatga atattcaatt ttaattgtaa attcattcat tactatatta
     7141 aacaattctt tcaataatgg tgttgaacag tgcctatctg gtgtccaaaa aacaaattta
     7201 cgccttatat tctcaatatt atattgaata tcatttggta tacaatttaa aaattttttt
     7261 aaaacagaaa tagtttcttg ctcaaatggc aaatcattat aatattctag tgactgatat
     7321 gcaaagaata tagattttac ctcactttct gaaaatgcaa tcggtggaag tatacgctct
     7381 ttaagtacat gatatccacc agccgctcca acttttgaat ataatggaaa tccaatactc
     7441 tccagcacct gtaaatctct tagtatagta cgttttgata cagaaaattc ttctgctaat
     7501 tctcctgctg taaaacttct ttttgcattt attgttatca tcagttcaat taaacgctca
     7561 gctcttgaca tatttatact cctaatttaa atatagtgac aataattgtc actattatat
     7621 gataagatat tcttgcatct aaagcaataa taatagtaaa gaaagaaggg attgatactt
     7681 tatggaaaat gatactatta aggctgatga tattctcaat tattgtctat caaacttaga
     7741 tgatgttgta ctaatggata gttgggggga acgagcaatt tactacaatc ctaatggtgt
     7801 tttaaagcga ggggtatatg ttcttaccat taaggaaaaa gacagtaata atgataaagg
     7861 ttcgttagtt agtcgtccaa atgtataccg tgtgaatata ggattaaaaa aagaaacttt
     7921 tattgaaatg tttggatata ttccaaagcg tccaggtgta ggtcaaatag ttgatatgga
     7981 ttttgatttt acaaaattgg acacaatcat gccacatcct atctactcat ggatgggatg
     8041 gatatgtgtc ctaagcccta ctgaaaagac atttgagaac tttaaaactt taataggaga
     8101 atcttacaat tttgcaaaac aaaaatttaa aaagagaaaa aacaagtaaa aacgtgtgaa
     8161 aataacctct aaataaatta taataaaaat attaaggtta caagataaaa attaatatct
     8221 taataacatg caaaaaagcg aagccaaaaa atgtattatt tataaggctt cgctatatct
     8281 caaataattt atttcaaatt tcaaaactat ttattctatt atttcttatt atttcctgtt
     8341 attcctaatc tatcattcat atcttttatc atttttctaa aaccactacc tatatcattt
     8401 ccaacatctt cagttccatg aactaatttt ataaagtcat tttgaccttt tttcatatct
     8461 tcatcagata ccttatctaa tttttctatt ctcttatcta tatcttttac taatctttcc
     8521 ccttcagcga ttaggtcatc attcattttc tttaactctg tatcttctgt tttaccatct
     8581 tttttaagag atgttacaaa tgattgtatt ttatctctac tatctttata tgcagccttt
     8641 acatcactta gatatttttc atttcctgga tattctttat ctttaaactg attatttata
     8701 ctttctggag tatcataaat agaatatcta tttagacctg ctatataatc attatagctt
     8761 gttgaataat aattcatata tccttcatta ttatttacag attcattagg attttttcct
     8821 gtattttcac ttggtttttc attattttta gtacatgcag ttatacctat agctaatact
     8881 agtgctaatc ctacaaataa agttcttttt aatttcattt ttattaccct cctagatgtt
     8941 ttatactata aaaaatttta ataattttct ttcttaattt agtatttcta tatttttata
     9001 ttttatttac taaaattaaa caagttataa tttattt
//
LOCUS       DAMJVK010000128         8994 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.133, whole genome shotgun sequence.
ACCESSION   DAMJVK010000128 DAMJVK010000000
VERSION     DAMJVK010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8994
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.133"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            155..496
                     /locus_tag="PC414_003348"
     CDS             155..496
                     /locus_tag="PC414_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator"
                     /protein_id="HDF2465719.1"
                     /translation="MKEENFINVEYEDKRFSINSKEVLYIEKQEYEVKIYTESGNYEV
                     GENAPCIRKLLKEENFFKCSNSYIVNLDKCEFYLSNNCIFVNNNFIRILGSERKKKLK
                     KKLEKLYDYED"
     gene            647..1060
                     /locus_tag="PC414_003349"
     CDS             647..1060
                     /locus_tag="PC414_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HDF2465720.1"
                     /translation="MLELKNNLGISERKLKYPCIYKHFKGKYYATMGLSKAIDDIENI
                     CEIYGKENLIQNRNKYKLVIRHTEREEDIYVYRDLDGNFYHKKEEDTNDLVLYKTLYD
                     DTGIFARPLDMFLEKVDTDKYVNSIQEYRFEEVYK"
     gene            1262..1903
                     /locus_tag="PC414_003350"
     CDS             1262..1903
                     /locus_tag="PC414_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDF2465721.1"
                     /translation="MGNTFYIVRHGQTDWNILGKTQGHGNSDLTPQGIEQAKELSEDI
                     GKYSIDYIFSSDLGRAMQTAQILGDKLNIEVQKTEALREMGFGVWEGLLIKEIQKDYS
                     DIYATWRNEPHLVNIPEGETLKIIKERVDAFIKELNEKYDNKNIILVTHSITLRVMLL
                     SFLESGMENIYRIKQDNTALNIVEFKDYGPVIVKMNDTSHIKNHVKINNSALE"
     gene            1927..3180
                     /locus_tag="PC414_003351"
     CDS             1927..3180
                     /locus_tag="PC414_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDF2465722.1"
                     /translation="MSKVIVVGGGASGMMAALSASKNNNEVILVERNGELGRKLRATG
                     GGRCNFTNNREIEDFFDKVVSNKKFLYSSFYTFTNKDLISYFESRNLEYKIEEENDHK
                     VYTKNDKSIEVIEVLNKDLLNHNVKIMYNKKVIDIITEEIALKDDSNKDKSKYLIKGI
                     ILDNGDKILGDKVIISTGGVSYSKTGSDGSMYKILKKHGHTLNKLYPALVPLTIEEKW
                     IKDLQGISMKNVEISCKIKKRKISKSGDMLFAHFGITGPCVLIMSSYINKIIEKEKVE
                     LNIDFLPNLNTDEISSIIRAFPNKNVLNNLKQILPQNFLKEIFSLLSLVDKKASDLSK
                     ADEIRIIEYIKNMKLTCNGTTGINTGMVTSGGISVKEINSSTMESKLVKNLFFTGEVI
                     DIDAETGGYNLQIAFSTGYLAGISV"
     gene            3226..3876
                     /locus_tag="PC414_003352"
     CDS             3226..3876
                     /locus_tag="PC414_003352"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HDF2465723.1"
                     /translation="MGNLVIAVDGPAGAGKSTIAKIVAKKLNINYIDTGAMYRAVTYK
                     CLKSGIDVNNEKEVIQIAENSDIDFKDNNIYLDKEVINEEIRTIEVSNNVSNVAKIKE
                     VRQLMVEVQRKIGMKNSVILDGRDIGSYVFPDADYKFFLVATPEERGNRRYKELCNKG
                     YNTTLEEVIEDIIRRDEIDSNREFAPLVKANDALEIDTTGKTIEEVVEEVVSKINL"
     gene            3927..4523
                     /locus_tag="PC414_003353"
     CDS             3927..4523
                     /locus_tag="PC414_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HDF2465724.1"
                     /translation="MNFYRFVINIFKGFSKIFFKYEVIGAENIPDRGNIVIASNHKSN
                     LDPIFLAAAIENREIAAIAKKELFKVKPLGFILKKLHVMPINREKPDVSTIKTILRSV
                     RDGYVLGIFPEGTRIKGDSFGKAKAGLSVFTIKSKSKVVPVSIISKYKLFSKVIVYIG
                     EPISFEEHFKEKLSNDDHERISQEILEVIKQNYFKYSK"
     gene            4536..5375
                     /locus_tag="PC414_003354"
     CDS             4536..5375
                     /locus_tag="PC414_003354"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HDF2465725.1"
                     /translation="MNIKIAKNAGFCFGVKRAMKMAWDEVEKNDSGIYALGPLIHNKQ
                     AVAKYEEKGLKTVNEIDTIPNHENMIIRSHGVPENIYKEAKDKKLKIVDTTCPFVKKI
                     HTVVSEYHNKGYEIIVIGDMKHPEVIGINGWCENSAIIIKTLEQMENMEFDNSKRYCL
                     VAQTTINPELYISIVNKLSDKLEEIVFNDTICSATKTRQESAKELAKEVDCMIVIGGK
                     HSSNTQKLVKVCEDLVPTFAIETKDELDVNTLKKYKNLGITAGASTPNWIIEEVVTFL
                     ENL"
     gene            5620..6774
                     /locus_tag="PC414_003355"
     CDS             5620..6774
                     /locus_tag="PC414_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic efflux protein"
                     /protein_id="HDF2465726.1"
                     /translation="MEMLISASEEAFLHVGSMIGFFILLFGYINYKTSGNFTNIISKN
                     RKFQPLIGALIGAIPGCGGSLAIMPLYINGKLSFGAIIASLIASMGDAAFVLISSNIK
                     MYFFVTIVSTITGIITGQLVDYFKLEEKLGLKNRRKSDKYDISKNNPKENKEQHNHDE
                     IILDTLAKSHGNTNRLAFIITHGKGYKIYIGIIVIGFIFMSLAHSGLNLPIIEKLHSL
                     EEVIAVIGILFSIIYMWCFKKVFKNSNQKEEENKKISLREMLIHSVGEISFVITWIFI
                     AYLIYDLIILILGGDEYLVKLVLSTGVISVFIGAGLGLIPGCGIQIVLMSFYLKGTIP
                     LGAVIANSISQDGDALFPLLAMDKKSSLWAMIITTIPAILVGMIVYAFLG"
     gene            7080..8777
                     /gene="ade"
                     /locus_tag="PC414_003356"
     CDS             7080..8777
                     /gene="ade"
                     /locus_tag="PC414_003356"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HDF2465727.1"
                     /translation="MGNLKRQCDVSSGREKADIVLKNGTIINVFTEELITGDVAIVGD
                     TIVGIGDYKGNVEIDCSNKYISPGFIDAHMHIESTMVMPIELSKKLLKSGTTTIIADP
                     HELVNVKGVSAIDFLLESTKDIPLNVYIMVPSSVPATSFETNGVGKFSAKDMESYVNN
                     PRILGLGEVMCFNDVINSENEILDKLELFKNKVVDGHAPNINGKSLQTYVCAGIENDH
                     ECITFDEVYEKLRAGLKILIREGSAAKNLKSIVSGMLKHNLPIEEFMFCTDDKHLDDI
                     EKQGHIRWNIKCAIDLGMEPVRAIKVATYNSAKAYGLRKIGAIGAGYKADIVVLNDLD
                     KMEVDSVYKDGNLVNEEMFSNYNYEIKDKELLNTVKFKYINKEKIQLKVSEKNYVMEI
                     VPYQILTNKVYESLPCADGYFVPNKEYSKLCVVERHRMTGNVAIAPLKGFGIKNGAIA
                     TTVAHDSHNIIVAGDNDDDILVAINYLKEIQGGYVIVSNGKVLAHLSLQVAGLISTFT
                     AEEVQEITDNMLEIARKMEVPEYVDPFITLSFMALPVVPQIRLTDLGLFDVEEFKFI"
BASE COUNT         3688 a          861 c         1579 g         2866 t
ORIGIN      
        1 tttaaaaatt aatgttaaaa aataaactta tacattaata ttaataataa ttatttaaat
       61 atcttaagta aatcagtctt acatatattt ccgatagatt atattgataa aaaacactaa
      121 aattaaatta ataaaagtac ttggagggat ggaaatgaaa gaggaaaatt ttattaatgt
      181 tgaatacgag gataaacgtt tttcaattaa tagtaaagaa gttttgtaca tagaaaagca
      241 agagtatgaa gtaaagattt atactgaaag tggtaattat gaagttggag aaaatgctcc
      301 ttgtataaga aaactattga aagaagaaaa ttttttcaaa tgcagtaata gttatattgt
      361 aaatctagac aagtgtgaat tttatctcag caataattgt atttttgtga ataataattt
      421 tataagaatt ttaggttcag aaagaaaaaa gaaattaaaa aagaaattag aaaaattata
      481 tgattatgaa gattaatatt gggaggctgt attttataca gcctctttat ttattgattg
      541 tattattaat aaattttaaa ttatgataaa atataaaaca agtaacttat ttcagaataa
      601 aatacttttt aaaagaaagg gacgagatat gggagattat aataaaatgc ttgaattaaa
      661 gaataactta ggtatcagtg aaagaaagtt aaaatatcct tgtatctata aacatttcaa
      721 agggaaatat tatgctacaa tgggtcttag caaggctatt gatgatatag aaaacatatg
      781 tgaaatatat ggtaaagaaa atttaatcca aaacagaaat aaatataaat tagttattag
      841 acatactgaa agagaagaag atatctatgt ttatagggat ttagatggaa acttttatca
      901 taaaaaagaa gaagatacta atgatttagt tttatacaag accttatatg atgatacagg
      961 aatatttgca agacctttgg atatgttttt agaaaaagta gatacagata agtatgtcaa
     1021 ttcaattcaa gaatacagat ttgaagaagt atataaataa tataagagaa atatgtttat
     1081 aaatttagaa aaattcttta acagtacaat aatttgcaga aatacaaatg agatttattg
     1141 taaataatat gttagattag attataaaat agaaacaaag taaagtatta tatgatataa
     1201 ttaagttttg agaagtgata atttatattt aataaaaaat aggcatatat taggaggaca
     1261 tatgggaaat actttttata tagtaagaca tggacaaacg gattggaata tattgggaaa
     1321 aacacaaggt catggtaatt ctgaccttac gcctcaaggt atagaacagg caaaagaact
     1381 ttctgaagat attggcaagt attcaattga ttatattttt agtagcgacc ttgggagagc
     1441 aatgcaaaca gctcaaattt taggagataa gctcaatatt gaagttcaaa aaactgaggc
     1501 acttagagaa atgggattcg gagtttggga aggcctttta ataaaagaaa ttcaaaaaga
     1561 ttattcagat atatatgcaa catggagaaa tgaaccacac ttagtaaata taccagaagg
     1621 cgaaactctt aagattataa aggaaagagt agatgctttt ataaaggaac taaatgaaaa
     1681 atatgataat aaaaatataa ttcttgttac acattcaatt acattgaggg ttatgttgtt
     1741 gtcattttta gaatcaggta tggaaaatat atatagaatt aagcaagata atactgcttt
     1801 aaatatagta gaatttaaag attatgggcc tgttatagta aaaatgaatg atacaagtca
     1861 tataaaaaat catgtaaaga taaataattc tgcactggag taggaatcaa ttataaggag
     1921 aaatttatga gtaaagttat agtagttggt ggaggagctt ctggaatgat ggcagctttg
     1981 tcagcttcaa aaaacaataa tgaagtaata ttagttgaga gaaatggaga attaggaaga
     2041 aaacttaggg caactggtgg cggtagatgc aattttacca ataatagaga gatagaagat
     2101 ttttttgata aagttgtcag taataaaaaa tttctctata gtagttttta tacatttaca
     2161 aataaagacc ttatttcgta ttttgaaagt agaaatttgg aatataagat tgaagaagaa
     2221 aatgaccaca aagtatacac taaaaatgat aaatctatag aagtaataga agtattaaat
     2281 aaagatttac tcaatcacaa tgtaaagata atgtataata agaaagttat agatattata
     2341 actgaagaaa ttgctctaaa agatgattct aataaggaca agtcaaagta tctaataaaa
     2401 gggattattt tagataatgg agataaaata ctaggagaca aggttattat atcaactgga
     2461 ggagtgagtt attcaaagac gggttcagat ggttcaatgt ataagattct taaaaagcat
     2521 ggacatacat taaacaaact atatcctgct ttagttccac ttacaataga ggaaaaatgg
     2581 attaaagatt tacaaggaat atccatgaag aatgtggaaa tatcttgtaa gattaagaaa
     2641 aggaaaatat caaaatctgg agacatgcta tttgcccatt ttgggataac aggaccttgt
     2701 gttcttataa tgtcttctta tattaacaag attattgaaa aggaaaaggt agaattaaat
     2761 atagatttct taccaaattt aaatactgat gaaatctcta gtataatcag agcatttcca
     2821 aataaaaatg ttctaaataa tcttaagcaa atactacctc aaaatttctt aaaagagatt
     2881 ttttctctat tatctttagt tgataaaaag gcaagtgatt tgtctaaggc agatgaaatt
     2941 agaattattg agtatataaa aaatatgaag ctaacttgta atgggactac aggtataaac
     3001 actggtatgg ttacttctgg tggtatttca gttaaagaaa ttaattcttc tactatggaa
     3061 tctaaattag ttaagaactt attttttaca ggtgaagtaa ttgatataga tgcagaaact
     3121 ggtggatata atttacaaat agcattttca actggatatc ttgcgggtat aagtgtataa
     3181 atataatttt ttaataatta aatatgaata aatagaggtg tatatatggg taatttagtt
     3241 atagctgtag atggtccagc aggagctgga aaaagtacaa tagccaagat agtagcaaaa
     3301 aagctaaata taaattatat agatacaggt gctatgtaca gagcagttac ttataagtgt
     3361 ttaaaaagtg gtattgatgt caataatgaa aaagaagtta ttcaaatagc tgaaaattcg
     3421 gatatagatt ttaaagataa caatatatat ttggacaaag aagttataaa tgaagaaata
     3481 agaacaatag aagtaagcaa taatgtttct aatgtagcaa aaataaaaga agtaagacag
     3541 ttgatggttg aagttcaacg aaaaatagga atgaaaaatt cagtaatact agatggtaga
     3601 gatataggtt catatgtttt cccagatgca gattacaaat tctttttagt agctactcca
     3661 gaagaaagag gaaatagacg ctataaagaa ctttgtaata aaggatataa tactacttta
     3721 gaagaagtaa ttgaggatat aataagacga gatgaaattg attcaaatag agaatttgca
     3781 ccattggtaa aagctaatga tgcactagaa atagatacta caggaaaaac tatagaagaa
     3841 gttgtagaag aagttgtatc taaaataaat ctttagtttt ttatgcatca taatgaaaat
     3901 aaatgtagtg ttgaagggag ataaacgtga atttttatag atttgtaata aatattttta
     3961 agggtttttc taaaatattt tttaaatatg aagttatagg tgcagagaat attccagata
     4021 gaggaaatat agtaatagca tcaaatcata aatcgaatct tgaccctata ttcttggctg
     4081 ctgccataga aaatagagaa atagctgcaa ttgcaaagaa agaattgttt aaagtaaaac
     4141 cattgggatt tatattaaaa aaattgcatg ttatgcctat aaatagagaa aaaccagatg
     4201 tatctacaat aaaaactatt ttaaggtcag ttagagacgg ttatgtatta ggaatatttc
     4261 cagagggtac tagaataaaa ggagattctt ttggtaaggc taaggcaggt ctttctgtat
     4321 ttacaataaa aagtaaatct aaagttgttc cagtatcaat aatatctaaa tataaacttt
     4381 ttagtaaagt aattgtatat attggagaac caatatcatt tgaagaacat tttaaggaaa
     4441 aattgagtaa tgatgaccat gaaaggatat cacaagagat tttagaagtg ataaaacaaa
     4501 attactttaa atattcaaaa taagggggta aagttatgaa cataaaaata gcaaaaaatg
     4561 ctggcttttg ttttggagta aaaagagcaa tgaaaatggc atgggatgaa gttgaaaaaa
     4621 atgattctgg catatatgct ttaggtcctc ttattcataa caagcaagca gtagcaaaat
     4681 atgaagaaaa aggtcttaag actgtgaatg aaattgatac aataccaaat catgaaaata
     4741 tgataataag gtctcatgga gttcctgaaa atatatataa ggaagccaaa gataaaaaat
     4801 taaaaattgt ggatactaca tgtccatttg taaagaaaat acatacagta gtaagtgaat
     4861 atcataataa aggctatgaa ataatagtta ttggagatat gaagcatcca gaagttatag
     4921 gtataaatgg atggtgtgaa aattcagcta taataataaa gaccttagaa caaatggaaa
     4981 atatggaatt tgataactcc aaaagatatt gtcttgttgc acagactaca ataaatccag
     5041 agttgtatat aagtatagtt aataaactat cagataaatt agaagaaata gtatttaatg
     5101 atacaatttg ttcagctaca aaaacaagac aagaatcagc taaagagtta gctaaagaag
     5161 ttgattgtat gatagttata ggaggtaaac acagttcaaa tactcaaaag ttagtaaagg
     5221 tttgtgaaga cttagtacca acttttgcaa tagagactaa agatgaattg gatgtaaata
     5281 cgcttaaaaa atataaaaat ttgggtatta cagcaggggc atctacacct aattggataa
     5341 tagaagaagt tgtaacattc cttgaaaatt tataatttga taggtaataa aaataggtgg
     5401 acattgttat aatttatcat aatatccacc tttgatttca tattacatgt ggtaaataga
     5461 taacctaata tcaaaaagtg tgtatcttaa gatgaaaaac taagagtata agatgcgcac
     5521 tttttaatat tagaaattca aaagaaatat tgatattgat tatcaataga aaatagaata
     5581 taatataatt gttgcaaaat tagtctagga ggtaaattaa tggaaatgtt gatatccgct
     5641 tctgaagagg cgtttttaca tgtaggttct atgattggat tttttatttt gttatttgga
     5701 tatattaatt ataaaactag tggaaatttt actaatatca tatcaaaaaa cagaaaattt
     5761 caacccttaa taggtgcttt aattggagct ataccaggtt gtggcggaag tttggcaata
     5821 atgccattgt atataaatgg aaaattaagc tttggagcaa taattgcaag tttaatagct
     5881 agtatgggag atgcagcatt tgtacttatt tcatcaaata ttaaaatgta tttttttgtc
     5941 actatagtta gtacaattac aggaataata actggacaat tagtggatta ctttaaacta
     6001 gaagaaaaat tagggttaaa aaaccgtaga aaatcagata aatatgatat aagtaaaaat
     6061 aaccctaaag aaaataaaga acaacataat catgatgaaa taattttaga cactctagct
     6121 aaatctcatg gaaatacaaa tagacttgca tttataataa cacatggaaa gggatataaa
     6181 atttatatag gaattatagt aatcggtttt atatttatgt cacttgctca ctctggactt
     6241 aatcttccta taattgagaa gttacattca ttggaagaag taatagcagt gattggaata
     6301 ttattttcaa taatttatat gtggtgtttt aaaaaggtat ttaaaaattc taatcaaaaa
     6361 gaggaagaaa ataaaaagat ttctttgcga gaaatgctta tccattctgt tggagaaata
     6421 tcatttgtaa ttacatggat ttttattgca tatcttattt acgatttgat aatattaatt
     6481 ttaggtggag atgaatactt agttaaatta gtattatcaa caggtgtaat aagtgtattt
     6541 ataggtgcag gtcttggact tattccaggt tgtgggatac aaattgtgct tatgtcattt
     6601 tatttgaagg gaactatacc acttggtgca gtgatagcca actctatttc tcaagatgga
     6661 gatgcacttt tcccactttt agcaatggat aaaaaatctt ctttatgggc tatgattatt
     6721 actactatac cagcaatact tgtaggtatg atagtttatg catttttagg ttaaatataa
     6781 ttatgaataa agaagtatta aaaaaataat aaagcacata aatttattta taagaatagt
     6841 gaagattaaa tataaaaata aaaaattcaa tatgaattaa attattgctt aaatattaat
     6901 gagaacatgg gagataatat tattcctcat gttctctcta tttaattaaa ggaattatgg
     6961 aacaaatttc aagtgatact ttctatcaaa actcatatat aatatatatt aataaattaa
     7021 actataatac gtaatacagt cagtatattg atataaaaaa cttaggagga gttgtaaaaa
     7081 tgggtaattt aaaaagacag tgtgatgttt caagtggaag agaaaaagct gacattgtac
     7141 taaaaaatgg gacaatcata aatgtattta cagaagagtt aataacagga gatgtagcaa
     7201 tagtaggaga cactattgtt ggaattggcg attacaaggg caatgtagaa attgattgta
     7261 gtaacaaata tatatcacct ggctttattg atgcacatat gcatattgaa tctacaatgg
     7321 taatgccaat tgaattatcg aaaaaattat taaaatcagg tacgacaact atcatagccg
     7381 acccacatga gttggtcaat gtaaaaggag ttagtgcaat agatttttta ttagaatcga
     7441 caaaagatat accacttaat gtatatatta tggtgccttc atccgtacct gcaacaagtt
     7501 ttgaaactaa tggagttggt aaatttagtg caaaagacat ggagagttat gtaaataatc
     7561 ctagaatttt aggtttagga gaggtaatgt gttttaatga tgttataaat tctgaaaatg
     7621 aaatcttgga taaactagag ctttttaaaa ataaagttgt agatggacat gcacctaata
     7681 taaatggtaa atcacttcag acatatgtat gtgcaggaat agaaaatgac catgaatgta
     7741 taacttttga tgaagtatat gaaaaattga gagcaggatt aaaaatttta ataagagaag
     7801 gaagcgcagc aaaaaattta aagtctatag taagtggtat gctaaagcat aacttgccaa
     7861 ttgaagaatt tatgttttgc acagatgata agcacttaga tgatatagaa aaacaaggtc
     7921 acataagatg gaatataaaa tgtgcgattg atttaggtat ggaacctgtt agggctataa
     7981 aagttgcaac ttataattct gcaaaagcat atgggttaag gaaaattgga gcaataggtg
     8041 cgggttataa ggcagatatt gttgtattga atgatttaga caaaatggaa gtggatagtg
     8101 tatataaaga tggtaatttg gtaaacgaag agatgttttc taattacaat tatgagatta
     8161 aagataaaga attgcttaat acagtcaagt ttaaatatat taataaagaa aaaatacagt
     8221 taaaagtaag tgaaaaaaat tatgtaatgg agattgtacc ttatcaaatt ttgacaaata
     8281 aggtatatga atctttacca tgtgcagatg gatattttgt tccaaataaa gagtattcaa
     8341 aactttgtgt tgttgagaga catagaatga ctggaaatgt agcaattgct ccactaaaag
     8401 gatttggcat aaaaaatgga gcaattgcta ctactgtggc ccatgattct cataatatta
     8461 tagttgcagg ggataatgat gatgatattc tagtagctat aaattactta aaggaaatac
     8521 aaggtggtta tgtaattgta tcaaatggaa aagttcttgc acacctaagt cttcaagtag
     8581 ctggattaat tagtactttt acagctgaag aagtacaaga aattactgat aatatgttgg
     8641 agatagcaag aaaaatggaa gttccagagt atgtagaccc ttttattaca ctttctttta
     8701 tggcacttcc agttgttcca caaataagac ttacagattt aggtctattt gatgttgagg
     8761 aatttaaatt tatatagata gtataaaagt aataataaat gaggctttct caaaatagag
     8821 ataagtctct ttttattgtt gaaatgatga aaatatattt gatttcatac aaaaaagagc
     8881 atctcatgaa ctaaaaattt tatttttaga tatccttatt ttaattaatc tatcatttaa
     8941 cattgtttta agcatagata tcttaataaa actattttca gtctatttta acat
//
LOCUS       DAMJVK010000129         8918 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.134, whole genome shotgun sequence.
ACCESSION   DAMJVK010000129 DAMJVK010000000
VERSION     DAMJVK010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8918
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.134"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(373..1644)
                     /locus_tag="PC414_003357"
     CDS             complement(373..1644)
                     /locus_tag="PC414_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDF2465728.1"
                     /translation="MLKVLKGNIIFTKTSETFTVFENSYIMLEKGKVKGIFKEIPKEY
                     KNLKVVDYTDKLIIPGMNDLHAHASQFKNLGMAMDKELLPWLETYTFPEESNYKDIEY
                     ATKMYKKFVKELWKSGTTRIAVFATVHKEASMKLMDIFKEVGLGAYVGKVNMNMNCPD
                     TLLENTQDSITDTEEIINKYSDTDSIVKPIITPRFIPSCTSELLKGLGDLCLKYNIPI
                     QSHLSENYDEIEWVRSLEPESKFYGDAYNRYNLFGQTPTLMAHCVHCSQDEIDLMREN
                     NVMAVHCPASNFNVGSGAMPIRKLIDNNIRLALGSDISGGHTLSIFKAMVSAIQLSKL
                     YWVNSGKKYNFLSLSEAFYIATKSGGSFFGKVGSFEEGYDFDALIIDDSNLNHDNYSI
                     LERLERFIYVGDDRNIIHRYVCGKLIEEPNI"
     gene            1856..2095
                     /locus_tag="PC414_003358"
     CDS             1856..2095
                     /locus_tag="PC414_003358"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465729.1"
                     /translation="MECYLKRTLIIFSEVFLSISFKTRKYLFIVIFILVCPNLSEMIF
                     ILVLARRRTIRIDNIVSIYKYVGVLKLINYSILIL"
     gene            complement(3886..4437)
                     /locus_tag="PC414_003359"
     CDS             complement(3886..4437)
                     /locus_tag="PC414_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2465730.1"
                     /translation="MSINTVIAKNLNRLRNERNLSLGQLAELSGVSKVMLSQIEKGDS
                     NPTVNTIWKIASGLNVPYTAILEQPQNETFIVSKTDIDVQVSENKDYCLYCYYPNTPT
                     RNFELFQMELEEGHSYTSVGHSEKSQEYIMIIEGQLKLEVNDSIYQLRENDSICFSAE
                     SIHTYHNQGEKTLKAVIINYYPV"
     gene            4628..5590
                     /locus_tag="PC414_003360"
                     /pseudo
     CDS             4628..5590
                     /locus_tag="PC414_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896810.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
     gene            5885..7729
                     /locus_tag="PC414_003361"
     CDS             5885..7729
                     /locus_tag="PC414_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDF2465731.1"
                     /translation="MQKRILRNFIFTGKNILSKLVFIIKYNFSTLILFELFHKGLALF
                     FIWPSIRYIFDILIKSLHIVYLNMDNFIKVLTNPMSIVLIILSVIILAFYAFFEFTSV
                     IICFNKSIKYEKIGLFELFKISFKNSIKLLYPKNILLFIFVILIIPLVNTVLISGFIG
                     KIQIPEYILDYIKSDIILNIIYMIFLLIIYISVIRWIFSIHEITLNTENFKRARKESV
                     NLIKGKIIRTIIYSLILSLVVAIIGYAIYHSGIIFIGIWTKYCTDMEFLKNVFINRCI
                     AFEEYFRLILSIFIFILDIGFISATYYQYKGVNISKNNIKKEKKSISKIVFNLVLILL
                     ISYIESVAFSSHINGMFNTSFSFYTTAIAHRAGAEMAPENTLAAIEEASHAKADYAEI
                     DVQETKDGELIVLHDSNFKRTTGVDKNVWDVNYNEVKTYDAGSFYYYGKGYYDEKFVG
                     EKIPTLKEMIKYSKGKVKLLIEIKLNGHEKGDVVKKVVQLIKENNFENQCIVASMDKT
                     ILQKVKKLDENLLTCYFMAIAYGDFSKLNYVDIYGIESTFVNKTVVEKIHKMNKKIFV
                     WTVNSDSLIEKMLDLNVDSIITDNPYLIESAIYWKKNGFISQVSDYLF"
     gene            8051..8635
                     /locus_tag="PC414_003362"
     CDS             8051..8635
                     /locus_tag="PC414_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDF2465732.1"
                     /translation="MTEREKMLAGELYDCGDIELLTQWHKAKGLVRDYNQTDSSDLEK
                     KEKILNELLGGKGKNLWITAPYYVDYGNNIYFGNNCEVNMNCTFLDDNKIIIGDNALI
                     APNVQIYTAFHPTNAGDRFGNAKEDGSFEFCKTQTAPVTIGDNVWIGGGAIIMPGVTI
                     GDNVVIGAGSIVTKDIPSNMIAYGNPCRIIRENK"
BASE COUNT         3414 a          943 c         1282 g         3279 t
ORIGIN      
        1 aaattttagt attaataaaa aagatattac attaatatga aaatatagga gattagaagt
       61 gtttgataat tacagtacat gttagatata tcattgtaaa ttaacatagc tttgaatata
      121 ataatgttca gaaggcaata tttttgaggt ctgaatccaa taataatata gtcttatgat
      181 tgtagatatc aaaaaatgta agttactagg aatacgtttt gtagatttat atatgattaa
      241 taactaaagc tataaataaa caaaataaaa agagtatgtt tcaacctata gcttttagat
      301 ttaaaatata ctcttttatt aattatagtt gtataaatta accataatat ataattaaaa
      361 tttttattat aattatatat taggttcttc aattagtttt ccacatacat atctatgaat
      421 gatatttcta tcatcaccaa catatataaa tctttcaagt ctttcaagta tagaataatt
      481 gtcatgattt aaatttgaat catcaataat caatgcatca aaatcataac cttcttcaaa
      541 gctaccaact tttccaaaga aacttccacc acttttggtt gcaatataaa aagcctcaga
      601 taaagataag aaattatact tcttaccaga gttaacccaa tataatttag ataattgtat
      661 tgcagatacc attgctttaa atattgacaa tgtatgtcca ccacttatat ctgatccaag
      721 tgctaatcta atattattat cgattaattt tctgataggc atagcaccac taccaacatt
      781 aaagttagaa gctggacagt gaactgccat aacattattt tctctcatta aatctatttc
      841 atcctgagaa caatgaacac aatgagccat taaagtagga gtttgaccaa ataaattata
      901 tctattatat gcatctccat agaatttaga ttcaggttct agacttctaa cccattcaat
      961 ctcatcataa ttttcagata agtgtgattg aataggaata ttatatttta aacataaatc
     1021 tcctaaacct tttaaaagtt cacttgtaca gcttggtata aatctaggtg ttattattgg
     1081 ttttacaata gaatctgtat cactatactt atttataatt tcttctgtat cagtaataga
     1141 atcctgagtg ttttctaata aagtatcagg gcaattcata ttcatattta ctttaccaac
     1201 gtatgctcca agaccaactt ctttgaatat atccataagt ttcatagagg cttctttatg
     1261 tactgttgca aacactgcta ttcttgtagt tccagatttc caaagttctt ttacaaattt
     1321 tttatacatt tttgttgcat attctatatc tttataattt gattcttctg gaaatgtata
     1381 agtttcaagc catggcaaaa gctctttatc catagccatt ccaagatttt taaattgaga
     1441 ggcatgggca tggaggtcat tcataccagg tattataagc ttatcagtgt aatctactac
     1501 ttttaggttt ttgtattcct taggtatctc tttaaatatt ccctttactt tacctttttc
     1561 cagcataata taactatttt caaatactgt gaaagtttct gaagtttttg taaaaattat
     1621 atttcctttt agtactttta acatataggt ctccttagtt ttaaatttgt tttaatatat
     1681 gtaggtaaaa ttttgtttta caaatcaaaa ttttaccaca tgtattatat caaatctaaa
     1741 ctataaatac tattagataa tagcaaatat tagtgaaata cagttgttta ctattattat
     1801 atataactga catttaagtg acatttaaga aaaatataat gcctacttac ataaaatgga
     1861 atgttattta aagagaactt tgattatatt ttcagaagtt tttttatcca tatcgtttaa
     1921 aacaagaaaa tatctattca tagttatttt tatattagta tgtcctaatc tttcagagat
     1981 gatttttata ttagttctag ctagaagaag aactattaga atagataata tagtaagtat
     2041 ttacaaatat gtaggtgttc ttaaattgat aaattattcc attttaattt tatagtttga
     2101 attttatgat ataataaaaa tatataaatt ttgcagtgag cgatattttt gataaagtag
     2161 ggtttaacag ttgcaatgta agggattgag ggtgtatgat aaatgttatc aattgcacta
     2221 ctcatggttc actgcaaatt tgagagagtt gtatgtgtgt aagtactgaa aatacttagt
     2281 ttattttggg gttttatatt aactaagtgg tatgtaaata aataatgtgt tttatcatca
     2341 taactcctgc taaacatgtt ttatattaac tatgtggtat gtaaatcttg ttaatgcaaa
     2401 taaacaacat agcactatta atagttttat attaactatg cggtatgtaa atagggtttc
     2461 tgataaaatc ttcaaacatg taaaatatgt gttttatatt aactatgagg tatgtaaatc
     2521 ttatacttag ttagaactat atatcgacac aaatatgctt tatattaact atatggtata
     2581 taaattgcaa tttttattcg ttgtccaatc tctttgaaat ttgttttata ttaactaagt
     2641 ggtatgtaaa tttgtaacaa gtataggtaa aataccccaa taatttatac agcattttat
     2701 cctttaaaat ataattattt tttatcattt gtagtaaata attaccagat aacattgact
     2761 ttagttttaa tgattaaaat ataaaagtag aataattata aaaagtattg aaaaatttat
     2821 aaatatatat aataaaactt aatgacaaga tattagatat aaaaaataat taccttataa
     2881 atagattgaa atttatgaat attcatacta taatttaaat ataaggagat gccctttgaa
     2941 aataaaaatt aaaaaatatt taatgctact acaataggaa ctagaactac acttaataaa
     3001 tatacagaaa ttagatttgg ctcaatataa aatacaaata tagaatttag gtgtttttta
     3061 tgaaaaaatt tttatatgct ttatatagtt tcattgttat tatagctaat tttagattaa
     3121 aagaaaaaaa ttataacttt attttattag caatcttttt tatattgctg ttaattcaaa
     3181 atggagtttt aaaaacaggt tatcaaaata gagctacatt ttatcaagat aattatttta
     3241 atagagagga taaaaatgat aagataaaat ttatgactat aaagattttt atatttttgt
     3301 cattaccatt atgttttaat atactattca attatttaac ctcttaaatt atatttagat
     3361 ataggagtct tatgataaaa gtaagcttat gtgttatttg tagtaaatat aaagtgcata
     3421 tagtgataaa acaaaagttt catataaatg aagaaaaaaa tataaaataa tttatttaac
     3481 tacttaaaaa taaactaaaa gggataataa aatagctttt aggttatggt aataaaggta
     3541 tttaaaaaga gtgcataagc actccaattg tgagaaatac atacacaaat cattattaca
     3601 acaatgaaaa atgtacatat aagagacgtt ttgtatttgt tttaagtgat aatattttaa
     3661 tactaactaa ataattatca caatagtaaa aaagaaaaag gagtattttg tactccattt
     3721 gtcaaaaata taaaactttt atataagctt attataacat aattgataaa agtgtaaaga
     3781 tatataatga atttataaag aaaaataaaa taaaaaagat aaatcctaat caaaggattt
     3841 atctttttgt atagaaaata attattctat tattaaatgg catggctaaa ctggataata
     3901 atttattatt actgctttca atgttttttc tccttgatta tggtatgtat gaattgattc
     3961 agctgaaaaa caaatagagt cattttctct aagttgataa atagagtcat ttacttctaa
     4021 ttttaattga ccttctataa tcataatata ttcctgtgat ttttcagaat gaccaacaga
     4081 tgtataacta tgaccttctt ctaattccat ttgaaataat tcgaagtttc tagttggtgt
     4141 attgggataa taacaataaa gacaataatc tttattttct gaaacttgta catctatatc
     4201 agtttttgat acaataaaag tttcattttg aggttgttcc agtattgcag tatatggaac
     4261 atttagtcca cttgcaattt tccaaattgt gtttactgtt gggtttgagt ctcccttttc
     4321 aatttgcgaa agcattactt tacttacgcc agataattca gcaagttgac ctagacttaa
     4381 gttacgttca ttacgtaatc tatttaaatt tttagcaata acagtattaa tactcatagt
     4441 agctccttaa aatgtaaatt attgacaaat ataatttacg aatgtataat ttatttaaca
     4501 ttatttataa tttacaaatg tataatataa tgaacgttaa atacactgta catccgtaat
     4561 ataaattata ctgcataaag agttgttttt caacggatga tagttgagag attaaggggg
     4621 aattgtaatg cgtgtactta ataatgttac agaggctata ggcaatactc atatgttaca
     4681 tttatccaaa atagcagatg aatttggagt tcaaggtaat atttatgcaa agatggaata
     4741 cttaaatccg ggatttagta aaaaggaccg tgtagcatta caaatgatta ccgaagcaga
     4801 aaatgcaggt ttattagttc caggtcaagc agttgtggaa ttaacaagtg gtaatactgg
     4861 aacaggacta gcaatagctt gtgcatgtaa aggatataaa tttatagcgt gtatgtcaaa
     4921 aggaaattct atggaaagag caagaatgat gaaagcttta ggtgctgaag tagttcttgt
     4981 agaccaaatg cctaattcag ttcatggaca ggtatctggt gaagatttgg cacttgtaga
     5041 aattgaggct caaaaaatag caaaagaaag aaatgcattt agagcagacc aatttaattt
     5101 agaagcttgt aatcatgcac atgaaaagta tacagcagaa gaaatgtggg aataattgga
     5161 tggaaatatt gatgtatttg tagattttct tggaagtggt agtacttttg caggatgttc
     5221 aaaaacactt aaaaaacata attcttctat caaatgttat gttgttgaac ctgaaacagc
     5281 agcaatttat tcaggtaaag aaataactga ccaagggcat aaaatacaag gtggaggata
     5341 ttctatggat ttgccattag tagacaagaa acttattgat ggttatattc aagttacaga
     5401 cgaggaggca actgatgtag caagaaaatt agctaaatta gaaggtgtat ttgcaggatt
     5461 ttcttcaggg gcaaatgtat gtgcagcaat taagttgtta aagagcagtg aaaaaggaaa
     5521 aaatatagtt ttaactctta atgatagtgg tttaaaatat ttaagtactg atttatatga
     5581 agaattgtaa taatagcaca atagttatgc aatgtttttt aaattaagta tatagattta
     5641 aattataaaa agtatctcaa tgtaattttt gaaagttata aaggagatac ttttttattt
     5701 tgtaaatatg gagtatccta cttataagta ttaagctata tagatattga tgattagcta
     5761 tgcaataaaa aagagtatgt gtttttgcat ttatacatca taacttggaa taatacatat
     5821 taatgttcta aatgtattta aaacatatga taaaattaga acaaatagat acaaggagat
     5881 aaaaatgcaa aagagaattt taagaaattt tatatttacg ggtaaaaata ttttaagtaa
     5941 acttgttttt ataataaaat ataattttag tacattaatt ttatttgaat tatttcataa
     6001 aggattagcc ttatttttta tatggccatc tattagatat atatttgata ttttaataaa
     6061 aagtttacat atagtctacc taaatatgga taattttata aaagttttga ccaatcccat
     6121 gtcaatagtt ctgataatac tatcagttat aatactagct ttttatgcat tttttgaatt
     6181 tacctctgta attatttgtt ttaataaatc gataaaatat gaaaaaatag ggttatttga
     6241 actctttaaa atatcattta agaactctat aaaattgtta tatcctaaaa atatactatt
     6301 atttatattt gtgatattaa taattccatt agtaaataca gttttaattt cagggtttat
     6361 aggaaaaata caaataccag agtacatact agattatata aagtctgata taattttaaa
     6421 tataatctat atgatttttc ttttaataat atatatttcg gttattagat ggatttttag
     6481 cattcatgag ataactttaa atacagaaaa ttttaaaaga gcaagaaaag aaagtgttaa
     6541 tcttataaag ggaaagatta taagaacaat tatttattca ttaatattat ctttagtagt
     6601 agctattatt ggatatgcga tttatcatag tggaattata tttataggca tatggactaa
     6661 atattgtact gatatggaat ttttgaagaa tgtatttata aatagatgta ttgcatttga
     6721 agaatatttt agattgattt tatcaatatt catttttata ttggacattg gatttatctc
     6781 agcaacttat tatcaatata aaggagtaaa tatttctaaa aataatatta aaaaagagaa
     6841 aaaatctata tcaaaaattg tttttaattt agtattaata ttattgattt cttatataga
     6901 gtcagttgca ttttcttcac atataaatgg tatgtttaat acgtcatttt cattttatac
     6961 aacagcaatt gctcatagag caggagctga aatggctcca gagaatactt tggcagcaat
     7021 agaagaagct tcacatgcta aagctgatta tgcagaaata gatgttcaag aaactaaaga
     7081 tggagaactt attgtacttc atgattcaaa ctttaagaga actacaggag tagataaaaa
     7141 tgtttgggat gtaaattata atgaagtaaa gacatatgat gcgggaagct tttattatta
     7201 tggaaaaggt tattatgatg aaaagtttgt tggggaaaag ataccaacac tcaaagaaat
     7261 gataaaatat tctaaaggta aggtgaaatt attaatagaa ataaaactta atggacatga
     7321 aaagggagac gtagtaaaaa aagttgttca gttaattaaa gaaaataatt ttgaaaatca
     7381 atgtatagtt gcatctatgg ataaaactat attacaaaaa gtaaaaaaac tggatgagaa
     7441 tttattgaca tgctatttta tggcaattgc atatggtgat ttttcaaagt taaattatgt
     7501 tgatatatat ggaattgaat caacttttgt taataaaaca gtagtagaaa agattcataa
     7561 aatgaacaaa aaaatcttcg tttggactgt taatagtgac agtttaattg agaaaatgct
     7621 tgacctaaat gtagatagta ttattacaga taatccatac cttatagaga gtgcaattta
     7681 ttggaagaaa aatggattta ttagtcaagt gtcagattat ttattttaaa tattctataa
     7741 ttgtattatc cagtaaaaga gatattatta aaatcaataa tatctctttt taacataaat
     7801 aaggttgtta aattaaggtt attcttataa aaattgtgct tatcagctat aaacaaggaa
     7861 taaaagtgaa aaaattgctc tgagattatt acagttaaaa gagttttaag atgacagaga
     7921 gatagattgt caactgtata tttaataaat cttgttattt attaaatttt atataagtaa
     7981 aatatataaa ttaataaatc ttatgctaga atatatatga aaaacaatat aaatttggag
     8041 gattaaaaac atgacagagc gagaaaaaat gttggcagga gaactttacg attgtggtga
     8101 tattgagttg ttaactcaat ggcataaagc aaaaggccta gtaagagatt ataaccaaac
     8161 agattcctcg gatttggaaa aaaaagaaaa aatattaaat gagttacttg gtggtaaggg
     8221 taaaaactta tggattactg caccgtatta tgtagattat ggaaataata tttattttgg
     8281 aaataattgt gaagtcaata tgaattgtac gtttttagat gataataaaa ttatcattgg
     8341 ggataatgca ctaatcgcac caaacgtaca aatatatact gcatttcatc caactaatgc
     8401 tggtgataga tttggtaatg ccaaagaaga tggttcattt gaattttgta aaactcaaac
     8461 agctccagtt actattggag ataatgtttg gattggcggg ggtgctatta tcatgccagg
     8521 tgttactatt ggagataatg tagttatagg tgcaggaagt attgttacaa aagatattcc
     8581 aagtaatatg attgcatacg gaaatccatg ccgtattatt cgagaaaata aataaagtta
     8641 aattttcttg caaaatctca aaaaacacct ttcttacttt atacataggg tattttctaa
     8701 tactttcaca tccatattca tttgttaaat ttttgtggaa tgtagttttt atcacttcta
     8761 tcttacattt ccaccatttt tttataaaag tcattccttt atagatttat ttataatagt
     8821 atatattcta tttgatgttt gaataagcac ttatttttaa gtaaaatact aacttttagg
     8881 ttagtatttt gctatagtat gatgattaag taagaata
//
LOCUS       DAMJVK010000130         8839 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.135, whole genome shotgun sequence.
ACCESSION   DAMJVK010000130 DAMJVK010000000
VERSION     DAMJVK010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8839
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.135"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            141..908
                     /locus_tag="PC414_003363"
     CDS             141..908
                     /locus_tag="PC414_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaP domain-containing protein"
                     /protein_id="HDF2465733.1"
                     /translation="MQIIDININNIDNEHICCSISEKKGENCVFSKKAWMKNNFDKGL
                     VFKRFDVRGKAFIEYIPAENAWCPITADNYIFINCFWISGKFKGQGYSNLLLQECVDD
                     AKSKGKYGLVVLSSSKKMPFLSDAKYLKYKDFCLADTSEPNYELLYFPFTDNAPIPYF
                     KNCVKKRKLNENGIVLYYSNQCPHTEKYAPILKKIANSRGIDVNLIKFETAKEAQNAP
                     APFTTYSLFFNGEFVTNEILSEKKIEKFLDTNIKKFN"
     misc_binding    1126..1339
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1593..2627
                     /locus_tag="PC414_003364"
     CDS             1593..2627
                     /locus_tag="PC414_003364"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HDF2465734.1"
                     /translation="MNKIKVGIIGATGYVGAELIRLLMNHDKVEVTAIGSNSYVGKDI
                     VDIYPSIGYKNNMICIENEKVIDMCDVVFTALPHGVSEKFVIKAIKSKKKVIDLGADF
                     RIKDEEVYSKWYGVSFIDKILHKKAVYGLSEIYKEDIKDADIIANPGCYPTSISLPLM
                     PLLSSKLIKNNNIIIDSKSGLTGAGRELSISSHFTEVNENITAYKIGKHRHTPEIEQN
                     LSESCKEKVSVVFTPNLIPVNRGILSTIYCTKEDNISINDIHRKLTDYYEFKEFIEIL
                     PLDKVASLKNVRFSNKCVISLHENGDTLIICSAIDNMIKGAAGQAIQNMNIIFGIEEN
                     TGLKNIAPSF"
     gene            2668..3885
                     /gene="argJ"
                     /locus_tag="PC414_003365"
     CDS             2668..3885
                     /gene="argJ"
                     /locus_tag="PC414_003365"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HDF2465735.1"
                     /translation="MEIIKGGVTVSEGFFASGIHCGLRKNKEKRDLALVYSDVLCDAA
                     AVVTQNKVKGNPVYVTQEHIKNGKAQAIIVNSANANTFNGKEGLINAYKMAKFTSDKL
                     NIKESDVLVASTGVIGKPLNIDLIEENMDELVNNLSKQGHIGAREAIMTTDIIKKEIA
                     VAIMYGDKKITIGGMAKGSGMIHPNMATTLGFITTDANIDGVLLKEALKIAVDKSFNR
                     VSVDGDTSTNDMVMILANGKAKNDRINKKDEHYQVFLSALTYVCIELAKLVAKDGEGC
                     TKLIECYINGALSEEHAVKLAKTVISSSLVKTAVFGADANWGRILCALGYAGEEIDME
                     KVDIIFESMKGYIEVCKNGNGLDFNEEKAKKILEDDEISILVDLNMGNARGNAWGCDL
                     SYDYVRINGSYRN"
     gene            3900..4760
                     /gene="argB"
                     /locus_tag="PC414_003366"
     CDS             3900..4760
                     /gene="argB"
                     /locus_tag="PC414_003366"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HDF2465736.1"
                     /translation="MINIEKANTLIEALPYIEKHQGKTIVVKYGGSAMKKDGLKESVM
                     EDLVLMSYVGINIVLVHGGGAEINKMLAKVDIESKFVNGLRYTDEETMEIVKMVLAGK
                     VNKDLVNKIHTKGGKAVGLCGIDNNMILCDPYKNYELGFVGEIKKVNVELIESCLKSG
                     YISVIATIGVGDDGETYNINGDTAASAIAKELNADKLILLTDVPGLLREPDEEKSLIT
                     EVILEDVDKLFEEGIITGGMIPKIEGCVDALNNGVNRVHILDGRVPHSIITELFTDSG
                     IGTLIRKETE"
     gene            4790..5977
                     /locus_tag="PC414_003367"
     CDS             4790..5977
                     /locus_tag="PC414_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HDF2465737.1"
                     /translation="MNNDNTISKWNEYFIDTYNQPNFVIDYGEGSCFFDTNGNKYIDF
                     TSGYGVSSLGYSNSNLKNALKEQVDKLLHTSNLYFNEPVLYSGEKIINSSGMAKVYFC
                     NSGTEANETAFKIARKYSSDKYGNGRGTIISLKDSFHGRTMMSLMATGMDKYHKYFYP
                     LPEGFKYIERNNIEDLKNNLDSTVCAIILEAIQGEGGVNVLEKDYVLEIVKICQEKDI
                     VVIFDEVQCGIGRTGKLFGYEYFDVKPDIVTVAKGLGAGIPVGGVLVNKKLSKVLGKG
                     DQGTTFGGNLLAMVAASVVLDEISKDGFYNEVLEKGNYIRKSIESFNNKVILKTKGIG
                     LMIGIETNIESSIIEEKARKKGLLILTAGKNVLRFLPPLTISYKEIDEALEILKDILL
                     EIN"
     gene            6086..7108
                     /gene="argF"
                     /locus_tag="PC414_003368"
     CDS             6086..7108
                     /gene="argF"
                     /locus_tag="PC414_003368"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDF2465738.1"
                     /translation="MNNISLKGKSFLTLKDFTKEEIRYLLTLSSYLKTKKKVGIKGDL
                     LQGKNIALLFEKTSTRTRCSFEVAAHDEGAHVTYLGPNDCHMGKKESVSDTAKVLGRF
                     YDGIEFRGFKQETVESLAKYSGVPVWNGLTDEYHPTQILADFLTVIENVDKDLNKVKF
                     VYVGDARNNMGNSLMIGCAKMGIHFVALAPKELWPNEELVKEMKEIAKKSKGDIMLTE
                     NIDDVKGADVIYTDVWVSMGEEEQYEARINQLKDYQVNMDMIKKTENENVIFLHCLPA
                     FHDLETKVAQEIYEKFGLSELEVTNEVFNSKYSKVFEEAENRMHTIKAVMVSTMTDVN
                     MNNNLI"
     gene            7235..7852
                     /locus_tag="PC414_003369"
     CDS             7235..7852
                     /locus_tag="PC414_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HDF2465739.1"
                     /translation="MKKIILALTTFLLAISLTACSSAKPEDTIDSFFNSAKKFDFEGM
                     NKVMENNDEKYKDILKELDTKDPNAQYVLDYLKQNASKITYTIKDSEVKGDKATIKVE
                     CKFIDSTPLLQEIVAEAFTKMIGMSFSGQDLTDEKTTEMLVSIMKEKQKSVKETYVTK
                     NVEFECSKKDNKWIISSVNDAVADVLLSNLVTAGQEFSNSMNTLE"
     gene            complement(8072..8770)
                     /locus_tag="PC414_003370"
     CDS             complement(8072..8770)
                     /locus_tag="PC414_003370"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HDF2465740.1"
                     /translation="MNEKLIGILAGMGPRSTAPFVDLIIDECQSQYGAKYDDEFPKMM
                     IYSLPTPFYIDRPINHDLMKETIIDGLQKLESTGVNFIAMPCNSAHIYFKELKESINI
                     PILNIVEETVKKLPTISQKVTLFSTSSTFESTIYQNGIIHNGHEFIFKDEWQIKLNNL
                     IQNIKMDKENPHNIDIWNKLIEDVKNESIENIVIACTDLNVVLEKSYPSINIVDSSKC
                     LAESVVNKYLKLVK"
BASE COUNT         3562 a          859 c         1583 g         2835 t
ORIGIN      
        1 ttaataattc agataatctt aaaataatat tatgtaaaat aaaaatatat atgacatgat
       61 gttgttgtat tcaaaatatt aagataaaaa tatatagaat atatttaaaa attattatgc
      121 aattagaaaa ggagataagt atgcagatta ttgatataaa tataaataat attgacaatg
      181 aacatatatg ttgttcaatt tctgaaaaaa aaggagaaaa ttgtgttttt tctaaaaaag
      241 cttggatgaa aaataatttt gataaaggtc tagtctttaa acgatttgat gtaagaggta
      301 aagcttttat agaatatatt ccagcagaaa atgcatggtg tcctattact gcggacaatt
      361 atatatttat taactgtttt tggatatctg gaaaatttaa gggtcaagga tattctaatc
      421 tattgctaca agagtgtgtt gatgatgcaa aatcaaaagg taaatatgga ttagttgtgc
      481 tttcgtctag taaaaaaatg ccttttttat ctgatgctaa atatctaaaa tataaggact
      541 tttgtttagc agatacatca gaaccaaatt atgagctatt atattttcct tttactgata
      601 atgctcctat tccatatttt aagaattgtg taaaaaaaag aaaattaaat gagaacggta
      661 tagttttata ttacagtaat cagtgtccac atacggaaaa atatgctcct attttgaaaa
      721 aaattgcaaa ttctagaggt attgatgtaa atcttataaa attcgaaaca gcaaaagaag
      781 cacaaaatgc tcctgcacca tttactacat acagtctttt ttttaatggt gaatttgtaa
      841 ccaacgagat actttctgag aaaaagattg agaaattttt agataccaac attaaaaaat
      901 ttaactaaaa agagatgtaa atattgtatt ttgaaaatat attattttat aatttaatat
      961 attttggatg ttttttaaat tgacgaatga aatataaaaa ttatatcgca atttagatac
     1021 aaaatgctgg aaattaacca tatagaaaat atttccaaaa atttaaaaat tatattgaca
     1081 aagttaaaaa aataaactat catataaaac aataagaaaa taaattgaaa tcctgtgaaa
     1141 aagagagtag cataaagcta gttgatagag agctgggaat ggtggaagcc tagtaacgaa
     1201 ttttattgtg aatagagctt tggaggagat taccagaagt aatagtatgg taattggtga
     1261 atctcgcctt aagagattag agtgggtaag attatcttat caattagagt ggtaacgcgg
     1321 gtgtatattc gtctcttggt ctttgaccaa gaggcttttt taattttaaa tgtacatttt
     1381 gtagatactt tttaatttga gttttaatga ttattgtaat tttaccaata agagtggtac
     1441 cgcggaaaat cgcctcttag tatttataat gctaaggggt tttttgtttt aattagagta
     1501 ttttaatcta cgtattatca aatgattatc atatagtaaa gtggaaagat aggattttaa
     1561 gactatttga atataaaaat ggagggaaca aaatgaataa aataaaagtt ggtattattg
     1621 gtgcaactgg atatgtcggg gcagagttga ttagattatt aatgaatcat gataaagtcg
     1681 aagtgacagc gataggttca aattcgtatg ttggaaaaga tatagttgat atatatccaa
     1741 gtataggata taaaaataac atgatatgta tagaaaatga aaaagttata gatatgtgtg
     1801 atgttgtatt tacagccctt ccacatggag ttagtgaaaa gtttgtaata aaggcaatta
     1861 aatcgaaaaa gaaagttata gatttaggtg ctgactttag aataaaagat gaagaagtat
     1921 atagtaaatg gtatggagta tcttttatag ataaaatctt acataaaaaa gctgtatatg
     1981 gattaagtga aatatacaaa gaagatataa aagatgcaga tataattgca aatcctggat
     2041 gttatccaac tagtatttct ttaccattga tgccattact aagttctaaa ttgataaaaa
     2101 ataataatat aataattgat tctaaatcag gtcttacagg tgctggtaga gaattatcga
     2161 ttagtagtca ctttacggag gttaatgaaa atatcactgc atataaaata gggaagcaca
     2221 gacatactcc agaaatagaa caaaatttaa gtgaatcctg caaagaaaaa gtaagtgtag
     2281 tttttacacc aaacttaatc ccagtaaata ggggaatctt atcaactata tattgtacaa
     2341 aagaagataa tatttctata aatgatatac ataggaaatt aactgattat tatgaattta
     2401 aagaatttat agaaattctt ccattagata aggtagcttc tttaaagaat gttagattct
     2461 caaataaatg tgtaatatca ttacatgaaa atggagatac attgataatc tgttcagcta
     2521 tagataatat gattaaggga gcagcaggtc aagctataca aaacatgaat ataatatttg
     2581 gtattgaaga aaatacaggt ttaaaaaata ttgcaccatc attttagaaa taaaaaatac
     2641 taaactcata taattaggag gataaatatg gagattataa agggtggagt tacagtttca
     2701 gaaggatttt ttgcttcagg gattcactgt ggcttgagaa aaaataaaga aaaaagagat
     2761 ttagctttgg tttattcaga tgttttatgt gatgcagcag cagtagtaac tcaaaataaa
     2821 gtaaagggca atcctgttta tgttacacag gaacacatta aaaatggaaa agcacaagct
     2881 atcatagtaa atagtgctaa tgctaatacc tttaatggaa aagaaggact tataaatgct
     2941 tataaaatgg ctaaattcac cagtgataaa ttaaatatta aagaaagtga tgttttggtt
     3001 gcatctacag gtgttatagg aaagccatta aatatagatt taatagaaga aaatatggat
     3061 gaattagtga ataatctaag taaacaaggt catattggag caagagaagc tattatgaca
     3121 actgatatta taaaaaaaga aatagcagta gcaataatgt atggagataa aaaaataact
     3181 attggaggta tggcaaaagg ttctggcatg atacatccta atatggcaac aacgttagga
     3241 tttataacta cagatgcaaa tattgatgga gttttgctaa aagaagcttt aaaaattgct
     3301 gttgacaaaa gttttaatag agttagtgta gatggagata caagtactaa tgatatggta
     3361 atgattctag caaatggaaa agcaaaaaat gatagaataa acaaaaaaga tgaacattat
     3421 caagtatttt tgagtgcact tacatatgtc tgtatagaac ttgctaaact tgttgcaaaa
     3481 gatggagaag gatgtacaaa actaattgaa tgctatataa atggagcatt aagtgaagaa
     3541 catgcagtaa aattagcaaa aacagttata tcttcttcat tagtaaagac agctgtattt
     3601 ggagctgacg caaattgggg tagaatactg tgtgctttag gttatgcagg tgaagaaata
     3661 gatatggaaa aagttgatat tatttttgag agtatgaaag gatatattga agtatgtaaa
     3721 aatggaaatg gattagattt taatgaggaa aaagcaaaaa aaatccttga agatgacgaa
     3781 ataagtatat tagtagattt aaatatgggc aatgccagag gaaatgcatg gggatgtgat
     3841 ttaagctatg attatgtaag aataaatgga agttatagaa attaggagga tatactttaa
     3901 tgattaatat agaaaaagca aatacactta tagaagcttt accatatata gaaaaacatc
     3961 agggaaagac tattgtagtt aagtatggtg gaagtgcaat gaaaaaagat ggtctaaaag
     4021 aatctgttat ggaagacctt gtgttaatga gttatgttgg aatcaatata gttttagtac
     4081 atggtggagg ggcagaaatc aataagatgc tagcaaaagt ggatatagaa agtaaatttg
     4141 taaatggtct tagatataca gatgaagaga ccatggaaat tgtaaaaatg gtacttgctg
     4201 gaaaggttaa taaagactta gtaaataaaa tacatacaaa aggtggaaaa gcagtagggc
     4261 tttgtggaat agataataat atgattttat gtgacccata taaaaattat gaacttggat
     4321 ttgtaggaga aatcaaaaaa gtaaatgttg agcttataga atcttgtcta aagtcaggtt
     4381 atatttcagt aattgcaact ataggtgttg gagatgatgg agagacttat aatataaatg
     4441 gtgacacagc agcttctgct atagctaaag agttaaatgc agataaattg atacttctta
     4501 cagatgtccc tggtttgtta agagagccag atgaagaaaa atcattaata acagaagtta
     4561 tattagagga tgttgataaa ttatttgaag aaggtataat tactggaggt atgataccta
     4621 aaatagaggg atgcgtagat gccttaaata acggagtaaa tagggtacat attttggatg
     4681 gaagagttcc acattctata ataacagagc tatttactga tagtggaata ggtacattaa
     4741 taagaaaaga aactgaataa aattataagt ttaagagggg agaataagaa tgaataacga
     4801 caatacaatt tcaaaatgga atgaatattt tatagataca tataaccaac ccaattttgt
     4861 catagattat ggtgaaggaa gttgtttttt tgatactaat ggcaataaat atatagattt
     4921 tacaagtgga tatggagtat cttctcttgg gtatagtaat agtaatttaa agaatgctct
     4981 aaaagagcaa gtagataaat tattacatac ttcaaattta tattttaatg agccagtgtt
     5041 gtattcagga gagaaaataa ttaattcaag tggaatggct aaagtatatt tttgtaattc
     5101 tggaacagaa gcaaatgaaa cagcattcaa aatagcaaga aagtatagta gtgacaaata
     5161 tggtaatggt agaggaacta tcataagttt aaaagactct tttcatggta gaacaatgat
     5221 gtctttaatg gcaactggta tggacaaata ccataagtat ttttatcctc ttcccgaagg
     5281 ttttaaatat atagaaagaa ataatattga agatttaaaa aataatcttg attccacagt
     5341 ttgtgcaata attcttgaag cgattcaagg cgaaggtgga gttaatgttc ttgaaaaaga
     5401 ttatgttttg gaaatagtaa aaatatgtca agaaaaggat attgtagtga tttttgatga
     5461 agtccaatgc ggtataggaa gaactggtaa actttttggc tatgaatatt ttgatgtaaa
     5521 accagatata gttaccgtag caaaagggtt gggagcagga ataccagtag gaggagtatt
     5581 ggtaaataaa aaactttcaa aagtactagg aaaaggagac caaggtacaa cttttggagg
     5641 taatttatta gctatggtgg cagcttcagt tgtattagat gagatatcta aagatggctt
     5701 ttataatgaa gtattagaaa aaggaaatta tataagaaaa tctattgaaa gttttaataa
     5761 caaagttata ttaaagacaa aaggtattgg tctaatgata ggaattgaaa ctaatattga
     5821 atctagtatt atagaagaga aagctagaaa aaaaggattg cttattttga ctgctggaaa
     5881 aaatgtattg agatttttac caccattaac aatcagttat aaggaaatag atgaggctct
     5941 tgaaatccta aaagatatat tactagaaat taactaaata ttcaaaaaat agttgattaa
     6001 agataaaaat tagaagtaaa taaaaaagta aagtgctaac aagtttaatt gaatttaaaa
     6061 agtttataag aaggggcgaa ttaatatgaa taatataagt ttaaaaggta agagtttttt
     6121 gacattaaag gattttacaa aagaagagat tagatattta ttaactttat caagttattt
     6181 aaaaactaag aaaaaagttg gtataaaagg cgatttgcta caaggaaaaa atatagcttt
     6241 attatttgag aaaacatcaa caagaactag atgttcattt gaagtagcag ctcatgatga
     6301 aggagcacat gtaacttatc taggtccaaa tgattgtcat atgggtaaaa aagagtctgt
     6361 atcagataca gcaaaagttc taggaagatt ctatgatgga atagaattta gaggctttaa
     6421 acaagaaaca gttgaaagtc tagcaaaata ttctggtgta cctgtttgga atggacttac
     6481 agatgaatat catccaacac aaatactggc agatttttta acagtaattg aaaatgtaga
     6541 taaagatttg aataaagtta aatttgtata tgttggtgat gctagaaata atatggggaa
     6601 ctcactgatg ataggatgtg ctaaaatggg aatacatttc gtagcactag cacctaagga
     6661 actttggcca aatgaagaat tggttaagga aatgaaggaa atagccaaga aatccaaagg
     6721 agatataatg ttgactgaaa atattgatga tgttaaaggt gcagatgtta tatatacaga
     6781 tgtatgggtt tctatgggag aagaggagca atatgaagca agaataaatc aacttaaaga
     6841 ttatcaagta aatatggata tgataaagaa aactgaaaat gaaaatgtta tatttttaca
     6901 ctgtcttcca gcatttcatg atttagaaac taaggttgct caggagattt atgaaaagtt
     6961 cggattatca gaattagagg ttacaaacga ggtatttaat tcaaaatatt caaaggtatt
     7021 tgaagaagct gaaaatagaa tgcatacaat aaaagctgtt atggtttcta ctatgactga
     7081 tgtaaatatg aacaataatt taatatagat atgttttaat atagacttta ctttaatgaa
     7141 aatttataaa atattttaaa tatattattc aaaaactctc attatatgtt aaaataactt
     7201 atgtatcaac aaattaagag ggaggatttg agatatgaaa aaaatcatat tagctttaac
     7261 aactttttta ttagcaatat ctttaacagc atgttcaagt gcaaaaccag aagatacaat
     7321 tgatagtttt tttaacagtg cgaaaaagtt tgactttgaa ggcatgaata aggtaatgga
     7381 aaataatgac gaaaaatata aggatatatt aaaggaatta gatacaaaag acccaaatgc
     7441 ccaatatgtt ttagattatc taaaacagaa tgcatctaaa atcacatata caataaaaga
     7501 cagcgaggtt aaaggtgata aggctacaat caaagtagaa tgtaaattta tagattcaac
     7561 accattactt caagaaatag tagctgaggc atttacaaag atgattggta tgagtttttc
     7621 aggacaagac ttaactgatg agaaaacaac tgaaatgtta gtatcaatta tgaaagaaaa
     7681 acaaaaaagc gttaaagaga cttatgttac aaaaaatgta gagtttgaat gttctaaaaa
     7741 ggataataaa tggataataa gttcagtaaa tgatgcagta gctgatgtat tgttatctaa
     7801 tcttgttaca gcaggacaag aattttctaa ctctatgaat acacttgaat aatattgttt
     7861 aggtgtagta aagtaataaa tatatgatta tttttataaa taaacatata aatatttatg
     7921 aaggatacca tttttggtat cctttttatt aatataatga aatacatggt taatttctat
     7981 attgtcaaga aactatgata tatgttataa gtctatacta tttaaaataa acttataaca
     8041 tatatttttt ataatatagt gtatgaaaaa tttatttaac taattttaga tatttattga
     8101 caactgattc agccaagcat tttgatgaat caacaatatt tattgaagga tatgattttt
     8161 ctaaaactac atttaaatca gtacaagcaa ttactatatt ttctatagat tcatttttaa
     8221 catcttcaat aagtttattc caaatatcaa tattgtgtgg attttcttta tccattttta
     8281 tattttgaat aagattgttt aattttattt gccattcatc tttaaatata aattcatgtc
     8341 cattatgtat tataccattc tggtaaattg tagactcaaa agttgaacta gtcgaaaaaa
     8401 gagttacttt ttgtgagata gtaggtaatt ttttaactgt ttcttctaca atatttaata
     8461 ttggtatatt aatagactct tttaattctt taaagtatat atgtgctgaa ttgcatggca
     8521 ttgcaataaa atttacacca gttgattcaa gcttttgtaa tccatcaatt atagtttctt
     8581 tcataaggtc atgatttatt ggacggtcaa tataaaatgg ggtaggtaat gaatatatca
     8641 tcatctttgg aaattcatca tcatattttg ctccatattg agattgacac tcatcaataa
     8701 ttaaatcaac aaaaggggct gttgaccttg gacccattcc agccaaaata ccaattaatt
     8761 tttcattcat aaaaattatc tcctttatat ataaatatat ttatgtataa aataaaaatc
     8821 tataaaagca taaaattat
//
LOCUS       DAMJVK010000131         8738 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.136, whole genome shotgun sequence.
ACCESSION   DAMJVK010000131 DAMJVK010000000
VERSION     DAMJVK010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8738
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.136"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(82..1854)
                     /locus_tag="PC414_003371"
     CDS             complement(82..1854)
                     /locus_tag="PC414_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation-like protein"
                     /protein_id="HDF2465741.1"
                     /translation="MKWIYDDYEFENRANNLSWTIAGDYNESIDISEKDYSSKEIGLY
                     FAIIAGARRKYVDWDIVKKYLTNRIRKGYNKDVILGLIQVILNTIVEEKVIKDRPGIE
                     DIRRNAYKDIVSRFTKIHNDNIMDKIKYTFVLYSMDKHPALDSMTRRIVNDIRNIDTN
                     QDIMEILKALDVIYLTYFEYILNGEENSLIDENNVESVEVNFDTFADFMYEELYSDEE
                     ENIIESDIDSISSSMLVEGVGDFDDSKCSNSADRVIYVDEDTANKIYSKIEHYYGKTY
                     LSKGEIKKIETQNCRNVHEGCRIHFTDGVLRSECNNVFQLKYVTRQKENNLGKFREHA
                     RMYRQQIKRLKESLSRILIEENAKSRVYSDCGTILANRAWRVGRSNNNKIFYKDIENE
                     KGKYVIDILLDASGSQSRNQGNVAIQAYIIANALTEVGIPNRVMGFSSFLDYTILKRF
                     KDYDDSIKNSENIFEYFCAGNNRDGLAIKSICSGLINRDEENKILIVLSDGRPNDVKI
                     GKSSERTLRGEKAYRGIVGLRDTANEVRKARKQNILVLGVFTGKESELEAERLIYGND
                     FIYSRDITRFSDVVSMYLKKIIRN"
     gene            complement(1868..2797)
                     /locus_tag="PC414_003372"
     CDS             complement(1868..2797)
                     /locus_tag="PC414_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2465742.1"
                     /translation="MDIIDNLRVQGVDEKLIEDVLYFRNYYGLEKDLEYRVTKSKTYF
                     YGKDILSMCIAAILEEENILLSGPKATGKNLLADNLGEIFNRPQWNTSFHINTDSSTL
                     IGTDTFIDNEVKLRRGSVYECAINGGFGVFDEINMAKNDAIVVVHSALDYRRIIDVPG
                     YERVNLHPATRFIGTMNYEYAGTKELNEALVSRFMVIDIPPIEEDKLMMILKNEFSDA
                     DEEKLIHFAGIFLDLQLKSQNGEISSKAIDLRGLMASLKTIRRGLKPTLAINMGLTGK
                     TFDVYEKEMVGDVIKTRIPNKWESIDVFPISHI"
     gene            3018..3203
                     /locus_tag="PC414_003373"
     CDS             3018..3203
                     /locus_tag="PC414_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDF2465743.1"
                     /translation="MKYGCPEVAKKWGISERRVQKLCEESRIPGVSKLGYMWLIPKNA
                     EKPVDGRTKRRKEVQYE"
     gene            3196..3897
                     /locus_tag="PC414_003374"
     CDS             3196..3897
                     /locus_tag="PC414_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2465744.1"
                     /translation="MNSVLIIDDDKELCALMKKCVEQENLSAITAHCGAEGLHLVDKN
                     RHTLSLVILDIMMPDIDGFQVLKKIRETSNIPVIMLTAKNDEDDKVSGLRLGADDYLT
                     KPFSINELMARVNSLIRRYTMLNPAFITDTDCIIINGMIIDKTNRIVSVNNIPVQLTS
                     KEFDLLAFLASNKGRVFTKKQIYTQVWEEEYAFDDSNIMSFISKLRKKIEPDPNHPFY
                     ILTVRGVGYRFNKEA"
     gene            3899..4822
                     /locus_tag="PC414_003375"
     CDS             3899..4822
                     /locus_tag="PC414_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2465745.1"
                     /translation="MNTSVYLLLILVLSLIIIAYLVSVLLRVRIQLTLIKDALEDIKN
                     GNLNRRILTDENDITKQICYDINEIAINSQFQLIQQKQSEQAYKQLMTSLSHDVKTPL
                     ASLVGYLEAVESKIVVGEEKDEYIHVASNKAHYLKNFVENLFEWVKLDSKEQVFHFDI
                     FDLNELSRNIISDWIPVLESSHFEYEFDIPEIEYFLRIDANAYTRIINNLLQNIITHS
                     SGNKMTLRIFENKEQAQIIITDNGKGISSDNLPHIFERLYQCDHSRASKGNGLGLAIA
                     KELINAHKGNITANSTPGMGTEFTILLPKAL"
     gene            4979..5902
                     /locus_tag="PC414_003376"
     CDS             4979..5902
                     /locus_tag="PC414_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2465746.1"
                     /translation="MNDFVIETKQLTKIYGNQTVVDKVNLHAKKGRIYGLLGRNGAGK
                     TTIMKMILGLTSITYGEVDVFGENIKGREKQVYPRIGAIIETPGFYPNLTGTENLEIF
                     AKLRGTAAPNSVKRALEIVGLPYKNKKLFSEYSLGMKQRLGIANAILHDPELLVLDEP
                     TNGLDPIGIAEMRKFIRDLSVEHGKTILISSHILSEISLLADDIGIIDHGVLLEESSM
                     SELEKKNSKYILLQVSDIPKTSLILERQFNTKDYSVQDDYTLHLYNTNLDMGEINKAL
                     VMQNVTVISSQLCNDTLEDYFKKITGGEGIA"
     gene            5895..6632
                     /locus_tag="PC414_003377"
     CDS             5895..6632
                     /locus_tag="PC414_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2465747.1"
                     /translation="MLKLIQTEFLKLRRRKLIWLMLLSALIMPFFALLYFNYFGKTGV
                     EPIKFYKWSAFGYTLWIILPVVLGIFCTMLIHEENQYDMLKQLWIVPISKMGYFFSKF
                     FVVLIYSICFMLITAVASVLFSVLSGYVVFSWASILYLLKKCLEIGVITAFVMLPILA
                     IATSQKGYILPICITLVYAFSGFVIMTINMYLHPLSSMGVIIMRNKDIPGLIFSQEIN
                     VPLAFLCICIWSIVSVLFANTALGRRK"
     gene            6643..7416
                     /locus_tag="PC414_003378"
     CDS             6643..7416
                     /locus_tag="PC414_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2465748.1"
                     /translation="MWKLLWAECQKIRRSKIVWITVFASIMIAVIVFAGGQDVYNDPD
                     LHYGLKSVYDGSRYIDNAGWYMDAVQPWSTFFVLPAVIALLGSYIICREEEEDTIKSL
                     RLIPINETKLTIAKMIITFIFSILLYLLLFTITFLTESVLHFSDLSTKLVLSCMKEYF
                     LDGIGVFFAISPIIALVSRMKKGYWLALVFTEVYSIAGLFAGMSNVLQTFFPIAAIFN
                     ISGYYITTGEKIMGSIISLLLCACLSAFILKGLKHNEKN"
     gene            complement(7957..8565)
                     /locus_tag="PC414_003379"
     CDS             complement(7957..8565)
                     /locus_tag="PC414_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDF2465749.1"
                     /translation="MSVINSFILQYGLISVFVLIMIEYACFPLPSEVVLPLCGAIAAR
                     NHFGFLTILILSIIAGILGSIFCYTVGNWGGKSIINKIIEICPKAKKGIFASQDYFNK
                     YSSISVCVCRLIPLCRTYISFIAGIAGQNIITFVISSIVGITIWNTSLISIGYIFSES
                     WVRIMSHYNDYKFLVLIIPISIIFIGFVIKSFIHKKHHKIRL"
BASE COUNT         2890 a         1441 c         1255 g         3152 t
ORIGIN      
        1 ttctgtgaaa atttctacat tttttataaa ttttattcat ttttatattt ttatgtatat
       61 aaattctttt ttgtaagttt attaatttct aattatcttc tttaaataca ttgaaactac
      121 atcagaaaat ctagttatat ctcttgaata tataaaatca tttccatata ttaatctttc
      181 agcttcaagt tctgattctt ttcctgtaaa tactcctaat actaatatat tttgctttct
      241 agcttttctc acttcattgg ctgtatctct caaaccaact atacccctat aagccttttc
      301 tcctcttaat gttctctcac tactttttcc tatctttaca tcatttggtc ttccatcgct
      361 gagaactatc aggattttat tttcttcatc tctatttatt agaccactac atatagattt
      421 tattgccaaa ccatctctat tattaccagc acaaaaatat tcaaatatat tttcactatt
      481 ttttatacta tcatcataat ctttaaatct ttttaatatt gtatagtcta aaaaactaga
      541 aaatcccatc actctatttg gtatgccaac ttctgttaga gcatttgcaa ttatgtaagc
      601 ttgaattgcc acatttccct gatttctact ttgagagcca cttgcatcca ataatatatc
      661 aataacgtat ttcccttttt cattttctat atctttataa aatattttat tattattact
      721 tctaccaact ctccaagctc tattggctaa tatagtacca caatcagaat atactctact
      781 tttagcattt tcttctatta aaattcttga cagactttct tttaatctct ttatttgctg
      841 tctatacatt ctagcatgtt ctctaaattt tcctaaatta ttttcttttt gtcttgttac
      901 atattttagt tgaaatacat tattacactc acttctaaga actccatctg taaagtgtat
      961 tctacaacct tcatgaacat ttctacaatt ttgtgtctca atttttttaa tttcaccttt
     1021 actcaaataa gtttttccat aataatgttc tatttttgaa tatatcttat ttgcagtgtc
     1081 ttcatcaaca tatataactc ggtctgctga attgctacat ttagaatcat caaaatcccc
     1141 tacaccttct acaagcatgg atgaagatat tgaatctata tcactttcaa taatattttc
     1201 ttcttcatca ctataaagtt cttcatacat gaaatcagca aatgtatcaa aatttacttc
     1261 tacactttct acattatttt catcaataag actattttct tctccattta atatgtattc
     1321 aaaataagtt aaatatataa catctaatgc ttttaaaatc tccataatat cttgattggt
     1381 atctatattt cttatatcat taactattct tctagtcata ctatctaatg ctgggtgctt
     1441 gtccatacta taaagtacaa atgtgtattt aattttatcc ataatattat cattgtgtat
     1501 tttggtaaaa cgtgaaacaa tatctttata agcattcctt cttatgtctt ctatccctgg
     1561 cctgtcttta atgacctttt cttcaactat tgtatttaat ataacttgaa ttagacctag
     1621 tataacatct ttgttgtatc cttttcttat tctatttgta agatattttt taactatatc
     1681 ccaatctaca tactttcttc tagcacctgc aattattgcg aaatatagac ctatttcttt
     1741 tgaagagtaa tctttttctg atatatctat actttcattg taatcaccag ctattgtcca
     1801 tgataggtta ttagctctat tttcaaattc ataatcatca tatatccatt tcataatctc
     1861 acctatatta aatatgactt attggaaaca catctatact ctcccattta ttaggtattc
     1921 tagttttaat tacatctcca accatttctt tctcatatac atcaaaagtc tttcctgtaa
     1981 gtcccatatt aattgcaaga gttggtttta atcctcttct tatagtctta agagatgcca
     2041 taagaccacg taaatcaata gctttactag atatctctcc attttgagac ttcaattgta
     2101 aatctaaaaa tattcctgca aagtgtatta atttttcttc atcagcatct gaaaattcat
     2161 tttttaaaat catcattaac ttatcttctt ctataggagg aatatctatt accataaatc
     2221 tagataccag tgcttcatta agttcttttg ttcctgcata ttcataattc atagtcccaa
     2281 taaatcttgt tgctggatgt agattgactc tctcataccc aggtacatca attattcttc
     2341 tatagtctaa agctgaatgt accacaacta tagcatcatt ttttgccata ttaatttcat
     2401 caaacacccc aaaacctcca ttaatagcac actcataaac tgagcctctt ctaagtttaa
     2461 cttcattgtc tataaatgta tctgtaccta taagtgttga actgtctgta tttatatgaa
     2521 atgatgtatt ccactgaggt ctattaaata tttcccctag attatcagca agtaagtttt
     2581 ttcctgttgc ctttggtccc gataataata tattttcttc ttctaaaata gctgctatac
     2641 acatagacaa gatatcttta ccataaaaat aagttttaga ctttgttact ctgtattcta
     2701 aatctttttc taaaccataa taatttctaa aatacagtac atcttcaata agtttttcat
     2761 ctaccccttg cacccttaga ttatctatta tatccatatt tttacccctt ttatataaat
     2821 agtatttgtt tcatttctta tcaccatttt agcataaaag gatataagct aaaattgatt
     2881 ttttaatctc cccattttaa gtaaaaccat gcaccatttt atcactacaa aattaagagt
     2941 actacttact gatatttctt gattttgttc gtctatacgt atataatata tgctgataaa
     3001 ttatgaggaa aggatgattg aaatatggat gcccagaagt agcaaaaaaa tggggtattt
     3061 ctgaaagaag agtacaaaaa ctatgtgaag aaagtcgcat accaggagtt tcaaaactcg
     3121 ggtatatgtg gctgatacca aagaacgcag aaaagcctgt tgatggtaga acaaaaagaa
     3181 gaaaggaagt acaatatgaa tagcgttttg attatagatg atgacaaaga actttgtgct
     3241 cttatgaaaa aatgtgttga gcaagaaaat ttatctgcta ttactgcaca ttgtggtgca
     3301 gaaggtctac atttggtcga taaaaataga cacacactct ctcttgtaat tttggacata
     3361 atgatgccag atatagacgg ttttcaagtt ctgaaaaaaa ttagagaaac aagtaatatt
     3421 ccagtaatta tgcttactgc taaaaatgac gaggatgata aagtttccgg actacgttta
     3481 ggtgctgacg attacctaac aaaaccgttc agcataaacg aacttatggc tcgtgtcaat
     3541 tctcttattc gtcggtatac tatgttaaac cctgctttta taacagacac agattgcata
     3601 ataataaatg gcatgattat tgacaagaca aaccgaattg tttctgtcaa taatattcct
     3661 gttcaactta caagcaaaga atttgattta cttgcttttc tggcttctaa taagggacgg
     3721 gtgtttacca aaaaacagat ttatacacaa gtatgggaag aagaatatgc ttttgacgat
     3781 agtaacatca tgtctttcat cagtaaattg agaaaaaaga ttgaaccaga ccccaaccat
     3841 ccattttata ttttgacagt tcgtggtgtc ggttatcgtt ttaataagga ggcttaacat
     3901 gaatacaagt gtctatctgc ttttgatatt ggttcttagc ctgattatta tagcgtacct
     3961 tgtttctgtc ctgctacgtg tccgaataca acttacactt ataaaagatg ctttagagga
     4021 tataaaaaat ggaaacctta atcgacgtat tttaacagat gaaaacgaca ttacaaaaca
     4081 aatttgctat gatattaacg aaatagcaat caatagccaa tttcaactta ttcagcaaaa
     4141 acaatcagaa caagcctaca aacagctaat gacaagtctc tcccatgatg taaaaactcc
     4201 acttgcttct ttggtcggtt atttggaagc agtagaaagt aaaattgttg taggagagga
     4261 aaaagacgag tatatccatg ttgcgtctaa taaggctcat tacttaaaga attttgtaga
     4321 aaatctattt gaatgggtaa aattggattc taaggaacaa gtttttcatt ttgatatttt
     4381 tgacttgaat gaattatccc gtaatattat atctgattgg atacctgttt tagaaagcag
     4441 tcattttgaa tatgaatttg atataccaga aatagagtac ttcttacgta tagacgcaaa
     4501 tgcatatacc cgtatcatta ataacctatt acaaaatatt attacccata gtagtggcaa
     4561 caaaatgaca ttgagaattt ttgaaaacaa agagcaagct caaattataa taacagataa
     4621 tgggaaaggt atatcttcag acaatctccc acatattttt gaaaggttgt accaatgcga
     4681 ccattcacga gcttcaaagg gaaatggttt aggcttggcg attgcaaagg aacttattaa
     4741 cgctcataag ggaaatatta ccgccaacag cactcccggt atgggaacag aatttacaat
     4801 attgcttcct aaagccctgt aaaagtacga ggctttctta ctctaaaaca agataaaagc
     4861 aagattatgg caaggttttg gcaaggttca tgttttataa ttctaattgt agttgatttt
     4921 gacgttgtat caactatctt ttgtagaaac aaatttatta aaagtgagga aattatatat
     4981 gaatgatttt gtgattgaaa caaagcaact tacaaaaatt tatgggaatc aaacagtagt
     5041 tgacaaagtc aatctccatg caaaaaaagg ccgaatatat ggtctgttag gacgcaacgg
     5101 agctgggaaa accacaatta tgaaaatgat tttaggattg acttcaatca cgtatggcga
     5161 ggtagatgta tttggagaga atattaaggg acgtgaaaaa caagtatatc cccgtatcgg
     5221 tgcaattatc gaaacgccag gattttatcc taatctgaca ggaacggaga acttggagat
     5281 ttttgcaaaa ctgcgtggta cagccgcccc taattctgta aaaagagcat tggaaattgt
     5341 aggcttacct tataaaaata aaaagctgtt tagtgaatat tcacttggca tgaaacagcg
     5401 acttggtatt gccaacgcta tcctgcatga cccagaactg ttagttttag atgagccaac
     5461 caatggtctt gaccctattg gtattgcgga gatgagaaag tttattaggg acttgagcgt
     5521 tgagcatgga aagacaattc ttatttccag tcatattctt tcagaaatct cattgttggc
     5581 tgatgatatt ggaattatcg accacggtgt tcttttggaa gaaagtagta tgagtgaact
     5641 agaaaagaaa aacagtaaat atatcctgct acaagtttca gatattccga agacatcact
     5701 tattttagag cgtcaattta acacgaagga ttattccgtt caagatgact acacgttaca
     5761 cctttataac actaatctgg atatgggtga aatcaataaa gcacttgtaa tgcagaatgt
     5821 aactgttatt agttctcaac tttgtaatga tactttagag gactacttta aaaaaatcac
     5881 aggaggagag ggcattgctt aaacttattc aaacagaatt tcttaaactg cgtcgtagga
     5941 aacttatttg gctgatgttg ttgtctgctc tcattatgcc gtttttcgca ctactatact
     6001 tcaattattt tggtaaaaca ggtgttgagc caataaagtt ttataaatgg tctgcatttg
     6061 gttatacact ttggattatt ttgcctgttg ttttgggtat attttgcacc atgcttatac
     6121 acgaagaaaa ccagtatgat atgctcaaac agctttggat tgtacctatt agtaaaatgg
     6181 gatacttttt tagtaaattt ttcgttgttt tgatttactc tatttgtttt atgctgatta
     6241 cagccgttgc ttctgtgtta tttagtgtac tttctggata tgtcgtattt agctgggcaa
     6301 gcattttata tttgttgaaa aaatgtttag aaattggtgt aatcactgct tttgtaatgt
     6361 tgccgatttt ggcaatcgct acatctcaaa aaggatacat tctcccaatt tgtatcacat
     6421 tggtatatgc cttttccggt tttgttataa tgacaataaa tatgtattta cacccattat
     6481 ccagtatggg agttattatc atgagaaata aagacattcc aggtcttata ttttcacagg
     6541 aaattaatgt cccattagca tttctttgta tttgtatttg gagtattgta tcagtattgt
     6601 ttgcaaacac tgcactagga agaagaaagt gaggtgtaga taatgtggaa attactttgg
     6661 gctgaatgtc aaaaaattcg tcgttcaaaa attgtttgga ttacagtttt tgcatccatt
     6721 atgatagcag tcatagtttt tgcaggaggg caggatgtat ataatgaccc agatttacat
     6781 tatggcttaa agagcgttta tgacggttca cggtatatag acaatgcagg ttggtatatg
     6841 gacgcagtac aaccttggtc aacttttttt gttcttcctg ctgtcattgc cctattagga
     6901 agctatatta tttgtcgtga agaagaggag gacactatta aatccctgcg tcttattccc
     6961 ataaatgaaa caaaattaac catagcaaaa atgattatta cttttatatt cagtatactt
     7021 ctttatttgt tactctttac tataacattt ttgactgaat ccgttttaca tttttccgat
     7081 ttatcgacaa aactggtttt aagttgcatg aaagagtatt ttcttgatgg cataggtgta
     7141 ttctttgcaa tttctcccat tattgccttg gtatcgcgca tgaaaaaagg atattggctt
     7201 gcattagtat tcacagaagt ttattctatt gctggactat ttgcgggtat gtcaaatgtc
     7261 ttacaaacct tttttcctat tgcggcaata tttaatattt ctggatacta tataactact
     7321 ggagaaaaaa ttatggggag tatcataagt ttattacttt gtgcctgcct ttctgcattt
     7381 atacttaaag ggctaaaaca caatgaaaaa aattgaaata ggtatatcaa aatgaaaaag
     7441 ataaaaaaac tgattattct aattgtattc ttgtttcttt ttggcatgtt acactgttat
     7501 actgaatgga aattcaacaa taacccacct aaaggggtaa tagaaagaat aaatgaatgg
     7561 actgataaga ttggactgga ttagtaactt atgttaatta tagtgaatgt aatattagca
     7621 agttattgtt tcgtatttta atactataac tttaaatatt tggttttaat cttccaaacg
     7681 aatataaaaa aactgtcaca gaggcaacat atttttgaat atttataaag tggtagtttt
     7741 gattttcaaa gctgccgcct tttcttctat ataagcaaac ttatcctcaa caaagtgtaa
     7801 aacatggtac attttaagaa aaaagtacca cagacacaag caataaaaga aacgccgaga
     7861 acctacaaaa acgtagtgct tatgcagatt tacgacgcct tctattttct caaccgtctt
     7921 aatgaccttg tacaaaagaa atttttctaa tattttttat aatcttatct tgtgatgttt
     7981 tttatgtata aatgacttaa taacaaatcc aataaaaatt atggatattg gtataattag
     8041 aacgaggaat ttataatcat tataatgtga cattattctt acccaactct cagaaaaaat
     8101 atatcctata ctgattaatg aagtattcca tatggttatt cccacgatag atgatattac
     8161 aaaagttata atattttgac ctgctatacc tgctataaaa gatatgtatg ttctacataa
     8221 agggattaat ctacatacac aaacagaaat agaagagtat ttgttaaaat aatcttgaga
     8281 tgcaaatatt cctttctttg cctttggaca tatctctatt attttattta tgatagattt
     8341 acctccccaa tttcccactg tataacaaaa tatcgaacca agtatacctg caattatact
     8401 taaaattaaa attgtcagaa atccaaaatg atttcttgca gcaattgctc cacataaagg
     8461 taaaacaact tcgctaggta atggaaaaca agcatactca atcataatta atacaaatac
     8521 agagattaat ccatattgta aaataaaact attgattaca gacacttact tatctatctc
     8581 ctttcttatt taaaggtttt aagggtacat gttctaagga ttctcaccct atatttaaag
     8641 aatcaatctc tttaaactat tatatgtatt tacacaaagc atttataaca tactttaaaa
     8701 atagtacctt aaaataagaa acttaataaa taaaaaaa
//
LOCUS       DAMJVK010000132         8724 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.137, whole genome shotgun sequence.
ACCESSION   DAMJVK010000132 DAMJVK010000000
VERSION     DAMJVK010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8724
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.137"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            231..1310
                     /locus_tag="PC414_003380"
     CDS             231..1310
                     /locus_tag="PC414_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HDF2465750.1"
                     /translation="MPELLKNMYNRESLYEVAVAIQSVYNSFKVDEFIKSTMDETWNN
                     LELKARCRKISMSLGMYLPEDYKEALSILEKSVTGFYFAFFFPDFVEVYGQDDINWDL
                     SISALERNTEYWSSEFAVRAFIIKDEERMMAQMRKWSKHKSEHVRRLASEGCRPQLPW
                     GQAISKFKKDPTPVLPILEQLKTDTSTYVQKSVANNLNDISKTHPDLVISIVKDWYGK
                     NKSTNWIVKHGCRTLLKKGNRDVLALFGYDDTTSINLQDFTLETTSISIGEDLTFSFN
                     ILAKKATKTRLEYGIDYIKSNGKRNRKIFKISEVSLKENEKKSYMKKHSFADVSVRKH
                     YPGIHSIAIIINGIEKDKLDFELGI"
     gene            1634..2002
                     /locus_tag="PC414_003381"
     CDS             1634..2002
                     /locus_tag="PC414_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HDF2465751.1"
                     /translation="MNVYSFWNDTLAQDAVKIRKYFHEDAYINWHDTNEHFTLEEFII
                     ANCEYPDKWNGKVERVLEFGNLVITVTHVYAINKSLSFHVVSFISLKDDKIISIDEYW
                     GEDDDVPQWRLDKHIGKPIK"
     gene            2119..2922
                     /locus_tag="PC414_003382"
     CDS             2119..2922
                     /locus_tag="PC414_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2465752.1"
                     /translation="MEEKYILSDRGKVYYWIKESNSVFAKNLIFLPGLTANHHLFDFQ
                     IQYFSKNHNVLVWDAPAHGKSRPYLDFSYSNLVEELKVILDRQNMKKVVLIGQSAGGF
                     VAQSFIVKYPNMVEGLMMIGTCPYGTDYYSKSDIFWLKQTEWMLKLFPDKLLRNIISK
                     MCGATSEGRRSMLKMLEGYEKKELCHLMYMGFAGFIPEIQDLNIHCPVCLVVGDKDKT
                     GKVRKYNEQWHNKTGYPLHIIKNASHNVNVDKPNELNQIMDIFIKQLNI"
     gene            3314..4204
                     /locus_tag="PC414_003383"
     CDS             3314..4204
                     /locus_tag="PC414_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2465753.1"
                     /translation="MIESIPAKQILQKVKFDNTRWFGIDYNMNLYRGCSHGCIYCDSR
                     STIYNIENFDKVRYKENVIEILSKELRSKRKKGVVGIGAMSDTYNPFEKQLCITKQAL
                     DLISENHFGVSIDTKSSLVVRDIPILQKIKKNNSAIVKLTITTANDELSKKIEPYVNP
                     SSVRFDAVKELNDSGIFCGILLTPMLPFLTDNKDEIRAIVEKAHKANAKFIYCMYGVT
                     MRSGQREFFYEHLRDISPKLVLKYQKTYGLNYVCTVQNKDSCEKLLREECARYGILTD
                     MKDIIKAYKRSESYTQIKFF"
     gene            4767..5210
                     /locus_tag="PC414_003384"
     CDS             4767..5210
                     /locus_tag="PC414_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2465754.1"
                     /translation="MIEIKTIEINKKSRIDLLMLADPCEEMIDKYLNKGTMYALYDNK
                     ELTCIAVVNEISKEICELKNIATYEHFQNMGYASKMIYHLLDVYSKKYSSMVVGTSES
                     GVPFYEKFGFVYSHKIKNFFVDNYPEPIFEGELQCVDMLYLSYNF"
     gene            5382..5552
                     /locus_tag="PC414_003385"
     CDS             5382..5552
                     /locus_tag="PC414_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465755.1"
                     /translation="MDSNNNTIKSTVKKGISFGSCLAMIISYTAWKSIPWAIFHGLMS
                     WIYVLYYWVKYA"
     gene            5925..7022
                     /locus_tag="PC414_003386"
     CDS             5925..7022
                     /locus_tag="PC414_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HDF2465756.1"
                     /translation="MKEKLEEKSKIIKTSKEYKRTIIEEKDREWTKKLNDILSRGDFS
                     NEKLDKIKDLIPKEILTSENVGEVVTEDGYAKPEYDEVFKSLFTLNNDYDLLANFIND
                     ILKDAPYANRNIKQFTQIKRIIKVETDPTINYIGEKRPRLDILAEDEERNHINIEMQR
                     ALEEDYLERAEYYLSRVHGRKLEEGKEYKEIGKTVGIHILNHVKYNHIEDYVNCLRLT
                     MDGHPDIYSSKTALYFIELPKIRKSSCIANRVLIWGKLIDNPSHIDIRILSKTDYVIK
                     RALDRLKELGSNEDYLLNLKRGAYIMNKSRNFKEEIFQEGIEKGIAKGKREEKFNIII
                     KLKNKGYNLSEICDIIDDLNKSEVEKVYNQN"
     gene            7227..7715
                     /locus_tag="PC414_003387"
     CDS             7227..7715
                     /locus_tag="PC414_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HDF2465757.1"
                     /translation="MKFYTIQTLEFWNNNKNNKYLSNDYSFILEDFDIPYKWMYSQMT
                     NRIYEFDDSMIWVWTKRPDLRCSGYLEKGKKGVLLEIEISEDNVLLSEFDLWHLPLMD
                     ITVELYEDEQIEKKVSWERIFDLELCSKLNEVNIEDLTLQGVTSKVPLSNVKLIKEFV
                     AK"
     gene            7801..8208
                     /locus_tag="PC414_003388"
     CDS             7801..8208
                     /locus_tag="PC414_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465758.1"
                     /translation="MSKQQTGIKSTLISEILGISYCIGATLVNKYFFKGSSIEYLPII
                     IWVLAMGAQWVYNKKYDDLIDEASKCILSRVNDIAIKVLFFSVAIVSIFLVTLDINVS
                     NLNIGMSLLVILFIQSLLKLILFIYFDRKGIYN"
     gene            8209..8427
                     /locus_tag="PC414_003389"
     CDS             8209..8427
                     /locus_tag="PC414_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2465759.1"
                     /translation="MSVLLKTKIKSYREKNLMTQGELAKSVGVRRETIVHLENGRYNP
                     SLKLAMDIAKVFDTTVEELFEFIEENNI"
BASE COUNT         3425 a          859 c         1539 g         2901 t
ORIGIN      
        1 cagtaaagta taaaattcta ttactataat aaaagataga actagaagtc tttatgaaag
       61 tgggaaaagt atttatataa ataaattcat atagtttata gtttaatagt aggaaatatc
      121 gtatgagtaa gaatattgta ttttacataa agatttagca atatgacaca ctgttgtcgt
      181 gttctgtagg atatgattaa taaatcagat atgaaaggag agtgagtaat atgccagaat
      241 tactaaaaaa tatgtataat cgtgagtcac tttatgaagt tgcagtagct atccaatcag
      301 tttacaactc atttaaggta gacgagttca taaaatctac aatggatgag acatggaata
      361 acttagaatt aaaagccaga tgtcgaaaga taagcatgag tttaggaatg tatttaccag
      421 aagactataa ggaagctctt tccattcttg aaaaatcagt tacaggtttt tattttgctt
      481 ttttctttcc agattttgtt gaagtttatg ggcaggacga cataaattgg gatttatcta
      541 taagtgcatt agaaagaaat actgagtatt ggtcttcaga atttgctgta agagccttta
      601 tcatcaagga cgaagagcgt atgatggcac agatgcgtaa atggtctaag cataagagcg
      661 aacatgtacg aagacttgca agtgaaggct gtcgtcccca gttgccttgg ggacaagcaa
      721 tatccaaatt taaaaaagac cctacacccg tactgcctat attggagcaa cttaaaacag
      781 atacatcaac gtatgtacaa aagagtgttg ctaataatct aaatgatatc tcaaaaacac
      841 atccagattt agtcataagc attgtaaagg attggtacgg taaaaataaa tccactaact
      901 ggattgtaaa gcatggttgc agaactctac taaaaaaagg caatcgtgat gtattagccc
      961 tttttggata tgatgatact acttctataa atcttcagga ctttacttta gaaacaacgt
     1021 ctatttctat tggagaggat ttaacttttt catttaatat tttagcaaaa aaagcgacaa
     1081 aaacaaggct cgagtacgga attgattata ttaaatctaa tggaaaaagg aataggaaga
     1141 tatttaaaat atcggaggtt tcattaaaag aaaatgagaa aaagtcatat atgaaaaaac
     1201 attcatttgc agatgtaagt gtaaggaaac attatccagg tatacactcg attgctatta
     1261 ttattaatgg tatagaaaaa gataaacttg attttgaatt aggtatataa ttttactaac
     1321 agaataatct atatcagata ctttgctaaa cttatataat atattttact aaatttatat
     1381 taggtagttt actacttgtt tactgaattt aaatacaaag aaatacaggt gctaatccct
     1441 gcccattaaa ggaatatgag cataacacaa aagtagaact tatcaattct gttacgggta
     1501 tagtagtaaa tccatagaat cacaattaga agatgaagtg taaaaaataa gttagtgttg
     1561 attttagttg ctagtgatat tgaatagtta tacaatagcc atataaatta gaatttgtag
     1621 aggagaaata taaatgaatg tatatagttt ttggaatgac actttggcac aagatgctgt
     1681 taagataaga aaatattttc atgaagatgc ttatattaat tggcatgata caaatgagca
     1741 ttttacttta gaagaattta ttattgcaaa ttgtgagtat cctgataaat ggaatggtaa
     1801 agtagaaagg gtgctagaat ttggaaattt agtaattaca gtgacacatg tttatgcaat
     1861 aaataaatca ctatcatttc atgtcgtttc atttatcagc cttaaagatg ataagattat
     1921 ttctattgat gaatattggg gagaggatga tgatgtgcca cagtggagat tagataaaca
     1981 tattggaaag ccaataaaat agtgtttgtt ctgttttata actgaaattt tgataacatt
     2041 tgatattttg atttgtcatt ggtcaacata gtcgttagtg taataattta aaaaagtaga
     2101 atttggagag tgaacactat ggaagaaaaa tacatcttat ctgaccgagg gaaagtatat
     2161 tattggataa aagaaagtaa tagtgtgttt gcaaagaact taattttttt gccaggacta
     2221 acagcaaatc atcatctttt tgatttccag atacagtact tttcaaaaaa tcataatgta
     2281 ttggtatggg acgctcctgc tcatggaaaa tcaagacctt acttagattt ctcatattct
     2341 aacttggtag aagaattgaa agtaatttta gatagacaaa atatgaaaaa ggttgtactt
     2401 atagggcaat cggcaggggg atttgtggca cagtccttta ttgtaaaata tccaaatatg
     2461 gtagaaggac taatgatgat tggaacttgt ccatatggaa ctgactatta tagtaagtca
     2521 gatattttct ggttaaaaca gactgaatgg atgttaaagt tattcccaga taaattgttg
     2581 cgaaacataa tatctaagat gtgtggagct acgtctgaag gcagaaggag tatgctgaaa
     2641 atgttagaag gttacgaaaa aaaagaactg tgtcatctca tgtatatggg atttgctggg
     2701 ttcataccag aaattcaaga cttaaatata cattgtccag tttgtttggt ggttggagat
     2761 aaagacaaga cgggtaaagt gagaaaatat aatgagcagt ggcacaataa gactgggtat
     2821 ccattgcaca ttataaaaaa tgcttcacat aatgtaaatg tcgataaacc taatgaacta
     2881 aatcagatta tggatatatt tattaagcaa ttaaatatat aaaaactaca tactcattag
     2941 gtaaattatg tagtatatgt gtttacagat ggttatagaa ataaatatgt aataaactac
     3001 ttaacactta aaaagtggat ttgtccagag cataatacaa agcatgatat atataaattt
     3061 ttaagcgaaa tacatcaaaa gaaggattag aattagtgga gacagaacag ctattataga
     3121 ttataaataa atagttttaa aggagaggta ctacgaatat agaggaaggt aaatcatacg
     3181 ttattcaagt attataaatg attagtttaa aagtactatc ggataggagt ctccatattt
     3241 aaaccttagt aaaggtagtt aaagtagtag tttaaaaact aaaaaattat ataaaggaat
     3301 taggtataag aaaatgattg agagtatacc agcaaaacaa attttacaaa aagtgaaatt
     3361 tgacaataca agatggtttg gaattgacta taatatgaat ctctatcgtg gttgttctca
     3421 tggatgtata tattgtgata gccgtagcac aatatacaac atagagaatt ttgacaaagt
     3481 tagatataaa gaaaatgtga tagaaatttt aagtaaagaa ttgcgttcaa aaagaaaaaa
     3541 aggtgtagta ggcattggag ctatgtctga cacatataat ccttttgaaa agcaactttg
     3601 tattacaaag caagcactag atttgatttc ggaaaatcat tttggagtgt ctattgatac
     3661 taaaagtagt ttggtggtta gagatatacc gattttgcag aaaattaaaa aaaataattc
     3721 tgctattgta aagttgacta taaccactgc aaatgatgaa ttatcaaaga aaatagaacc
     3781 ttatgtaaat ccaagttctg tacgatttga cgcagtgaaa gaacttaatg attctggaat
     3841 tttttgtggc atattattga ctcctatgct tccatttttg actgataata aagatgaaat
     3901 acgagcaatt gtagaaaaag cacacaaggc taatgcaaaa tttatttatt gcatgtatgg
     3961 tgtaacaatg cgtagtggtc agagagagtt tttttatgag catttaagag atatatcacc
     4021 aaaattggta cttaaatatc agaaaacata tggtttaaac tatgtatgta ctgtacaaaa
     4081 taaagattct tgtgagaagt tgttaagaga agaatgtgca cgttatggta ttttgacgga
     4141 tatgaaagat attataaaag cgtacaagag gtcggagtca tatactcaaa taaaattttt
     4201 ttaagatagg ctttggatga gatataatag tgttgattga tgtgttgata ttatagttga
     4261 ttgttagaga ggatattata gatgaatatt agtattgata ttttaggtaa ttgttagagc
     4321 tgatattata gatgaatatt agtattgata ttctaggtaa ttgctagagt tagtattata
     4381 gatgaatatt agtattgata ctctaggtaa ttgctagagt tagtattata gatgaatatt
     4441 agtattgata ttctaggtaa ttgctagagc tgatattata atttattatt agtattgata
     4501 ttctaggtaa ttgctagagc tgatattata atttattatt agtattgata ttctaggtaa
     4561 ttgctagagt tagtattata gatgaatatt agtattgata ttctaggtaa ttgctagagc
     4621 tgatattata atttattatt agtattaata ttctagggaa ttatcactat attttaaaaa
     4681 gtaaaaatat atatcaaaat gaattaaata agtactgtaa gaagtttgtt aaattaaggt
     4741 ttgaataaag tgagggtgga gaacttatga tagaaataaa aactatagaa ataaataaaa
     4801 aaagtcgaat tgacctttta atgttagccg acccatgtga agaaatgatt gataaatatc
     4861 tcaataaagg aactatgtac gcattgtatg ataataaaga acttacttgt atagcagttg
     4921 taaatgaaat ttccaaagaa atatgtgaat taaaaaacat tgctacatat gagcattttc
     4981 aaaatatggg ttatgcctca aagatgattt atcatctttt agatgtttat agtaaaaaat
     5041 attcatcaat ggttgttggt acatctgaaa gcggagttcc tttttatgag aagtttggat
     5101 ttgtttattc acataaaatt aaaaactttt ttgtagataa ttatccagaa ccaatctttg
     5161 aaggagaatt acaatgtgtt gacatgttgt atctatcata taacttttag ataggataat
     5221 atttttaggt taatataatt aattaattca tataattatc ggaattataa tttataaatt
     5281 atgtataata agagtataat ttatttagaa gctgttatat tgtattatat tcaaataaga
     5341 tatataataa cttgtttatt ttgaattttg gaggattaat tatggattca aataataata
     5401 ctataaaatc aactgttaaa aagggtattt cttttggttc ttgtttagca atgattattt
     5461 cttatactgc atggaaatct attccatggg ctatttttca tggcttaatg agttggatat
     5521 atgtacttta ttattgggtt aagtatgcat agattaaatt aataaataag tatgtaatat
     5581 tgtaaagttt aagaaacaaa atgtaaggga gtgtctcgaa gtgaactttt agtttataag
     5641 gcactctttt ttgtattaaa taaaatatat tttcattatt taaacaatga aaaagatgag
     5701 tatctctatt ttaatacata cccttatatt cattcattta tcaacaatag ttttaaaaag
     5761 aaagctatct aaaatctatt gtaatctaag tgcttctctt taaagtctta actttagtaa
     5821 tgatatataa gtttataagg ttcttatttc aataatgcaa attttactta gaagttcaga
     5881 ctttggtatg aagaaatggg taataataaa aaagaggtgt tgatatgaaa gaaaaattag
     5941 aagaaaaatc taagattatt aaaacatcaa aagagtataa acgaactata attgaagaaa
     6001 aggacagaga atggaccaaa aaattaaatg acatacttag tagaggtgat ttttctaatg
     6061 aaaaattaga taaaatcaag gatttaattc caaaagaaat attgactagt gaaaatgttg
     6121 gagaagttgt aactgaagat gggtatgcta agcctgagta tgatgaggta tttaaatcat
     6181 tgtttacttt aaataatgat tacgatttac ttgcaaattt tataaatgat atcctcaaag
     6241 atgctccata tgctaatagg aatatcaaac agttcactca aataaaaaga ataattaagg
     6301 ttgaaaccga cccaacaatt aattacattg gtgagaaaag acctagactt gatattttgg
     6361 ctgaagatga agaacgtaac catataaata ttgagatgca aagagctctt gaagaggatt
     6421 atttggaaag agctgaatat tatcttagtc gtgtacatgg tagaaagtta gaagaaggta
     6481 aagaatataa agaaattgga aaaactgttg gaatacatat actaaatcat gtaaaatata
     6541 atcacataga agattatgta aattgcctga gattaacaat ggacgggcat ccagatattt
     6601 attctagtaa aactgcatta tattttatag aacttccaaa aattcgtaaa tccagttgta
     6661 tagcaaacag agttctaata tggggtaaac ttattgataa tccttctcat atagatatta
     6721 gaatactatc taaaacggat tatgttatta aaagagcact tgatagatta aaagaattag
     6781 gtagtaatga ggactattta ttaaatttaa agagaggtgc gtatattatg aataaaagta
     6841 gaaattttaa agaagaaatt tttcaagaag gtattgaaaa aggtattgca aaagggaaaa
     6901 gagaagagaa atttaatatc atcataaaat taaaaaataa aggatacaat ttgagtgaaa
     6961 tatgcgatat tatagatgat ttgaataaat cagaggttga aaaagtctat aatcaaaact
     7021 aatactatga ttgagatggc actagataaa tacaaagctt cctcttttaa atttaaatta
     7081 atataaaact ttgttattta aatatggtag atataaagag atatataaat aataggtaca
     7141 aagaggtgta taaataaagg agataattca aaataaaagt ttgaaccatc tccttcttat
     7201 agaaaaacaa aggggttgat ttatatatga aattttatac tatacagact ttagaatttt
     7261 ggaataacaa taaaaacaat aaatatctat caaatgatta ttcttttata ttagaagact
     7321 ttgatattcc atacaaatgg atgtatagtc aaatgacaaa tagaatatat gaatttgatg
     7381 atagtatgat atgggtttgg acgaagagac ctgatttaag atgtagtggt tatttagaaa
     7441 aaggaaaaaa aggtgtttta cttgaaatag aaataagtga agacaatgtg ttattatcag
     7501 aatttgattt atggcattta cctttaatgg atattactgt agagctttat gaagatgaac
     7561 aaattgaaaa gaaggtaagc tgggaaagaa tatttgactt agaactgtgt tcaaaattaa
     7621 atgaagttaa tatagaagat ttgacattgc aaggagtgac atctaaagtg cctctatcta
     7681 atgtaaaatt aataaaagaa tttgttgcaa aataatattt ttattgaaat ttattcaaaa
     7741 aagttatata tttataacaa gaggtgttat aaatacataa ctttttaagg gggattttat
     7801 atgtctaaac aacaaactgg tattaaatca acacttattt cagaaatatt gggaatttct
     7861 tattgtattg gagctacatt agtaaataag tattttttta aaggaagtag tattgaatat
     7921 cttcctataa ttatttgggt attagctatg ggagctcagt gggtctataa taaaaagtat
     7981 gatgatttaa ttgatgaggc atcaaaatgt atactatcaa gagttaatga tattgcaata
     8041 aaagtattgt ttttttctgt agctatagta tctatatttc ttgtaacttt ggacataaat
     8101 gtgtcaaatt taaatatagg aatgtcttta ttagtcattt tgtttattca atccttgttg
     8161 aagttaattc tatttatata ttttgacaga aaggggatat acaattaggt gtcagtatta
     8221 ctaaagacaa aaattaaatc ctatagagaa aagaacctta tgacacaagg tgaacttgca
     8281 aagtcagtag gagtaagacg tgaaactatt gtacatttag aaaatggtcg ctacaatcct
     8341 tctttaaaac tggcaatgga tattgctaag gtatttgata caactgtaga agagttgttt
     8401 gaatttattg aagaaaataa tatttaaatt tcatgtatta cttatgcatt agagaatgaa
     8461 ttaattatag gtaaaaaacc aagcttatag attagagaat aaatgactta tagatgaaaa
     8521 accaagctca tagattaaag aatgaatgat ttatagatga aaaagaaagc ttatagatta
     8581 gagaataaat gatttatagg taaaaaccaa gctaatagat taaaaaatga atgatttata
     8641 gattaagaag taagctaata gattagagaa taaatgactt ataaataaaa accaagctaa
     8701 tagattagag aataaatgac tcat
//
LOCUS       DAMJVK010000133         8630 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.138, whole genome shotgun sequence.
ACCESSION   DAMJVK010000133 DAMJVK010000000
VERSION     DAMJVK010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8630
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.138"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(59..955)
                     /locus_tag="PC414_003390"
     CDS             complement(59..955)
                     /locus_tag="PC414_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2465760.1"
                     /translation="MKDKNTYFNRKGQENFIDIFLKDDIKKGANFKRHWHEHLQIYYF
                     IDGIAYLECGLDNFKVVPGNIVIANSNELHYLESLSDNLKFYSIRIEPTFLFSNQVDL
                     LQTKYLAPLALNHIVFNNLIQNDSLILKCITTILDEYHNKEVGYELSIKANIYQLIVL
                     LLRSHIDKIYSEDEFIKRKYSLEKFECIFDLIEEKYMDKITLKELASHVNLSAHHFCR
                     TFKQITGKTTIDFINGIRLDKAIYYLKETDLNMTEIAMRCGFDSINYFSRLFKRNYNI
                     SPTKYRNEHKEVSNVILKEDMK"
     gene            1096..2523
                     /locus_tag="PC414_003391"
     CDS             1096..2523
                     /locus_tag="PC414_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2465761.1"
                     /translation="MTNSFKKDDNRKKITVLFVVIAMTFMATLDSSIINVALPVLASK
                     LNVSLASIEWVIASYSIIICSTLLFFGRLGDIIGKSRVFQVGTILFTSASLLCGLSNS
                     LTLLIVCRFIQGIGASAYMANNHGIITELFPKESRGKALGILVTAVAIGNMVGPSVGG
                     FILSIFDWNVIFFINIPIGLIVIFLNTKFLPNSKKSSENMDKTGAILQFLGTTLFFSA
                     LISAQQTGLLNPYILIALLLSIIFIILFLILEKKHPQPLLDLEIFRNFKFSLNLICAL
                     TSFICIASSSILIPFYLQSTMKLPPIQAGLFMILSPLILAIFSPIFGNISDKIKSEKI
                     ILIGLLVMSFGFFLMSRLKESSALILFVIYISIISIGQAIFQPANNALIMSSCSRSKL
                     GVVGSINSLVRNLGQVIGITISTTLLYNFMSIKAGYRVNDYVINNDKIFVFGMRNVYI
                     IVTLVCLIGAILIGFYLFKYNKNEQ"
     gene            complement(2719..5313)
                     /gene="clpB"
                     /locus_tag="PC414_003392"
     CDS             complement(2719..5313)
                     /gene="clpB"
                     /locus_tag="PC414_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HDF2465762.1"
                     /translation="MDVEKMTLRVQKSLNEAYNEAVKNHNQQVDVIHLFSALINQEDG
                     LIPNIIEKMNISIDSLRNTVNFEIDKLPKVYGEGADSQGVSATRKINEVLIKAESISK
                     EFKDSYISVEHVMLAMMETESKSAVGKILKQYNINKNNFLNILSQVRGSQRVETQDPE
                     GTYDALARYGTNLVDLAKKNKLDPVIGRDEEIRRIIRILSRRTKNNPVLIGEPGVGKT
                     AIVEGLAERIVRGDVPEGLKDKIVFSLDMGALIAGAKYRGEFEERLKAVLKEVQSSDG
                     KIILFIDEIHTIVGAGKTEGSMDAGNLIKPMLARGELNCIGATTFDEYRKYIEKDKAL
                     ERRFQPVIAEEPTVEDTISILRGLKERFEIHHGVRIHDNAIVAAAKLSHRYIQDRFLP
                     DKAIDLIDEAGAMIRSEIDSLPTELDVVRRKLFTLETEREALLKENDDKSKSRLDDIQ
                     KEIAELKSKNDEMTAKYEKEKSQILDIKNLKAQLDEAKGKAEKYEREYDFNKAAEVKY
                     GEIPKLEEQIKQYEENMNDGSENSLLKEEVTEEEISSIVSKWTGIPVTKLVEGEREKL
                     LKLEDELHKRVIGQDEAVTAVSNAVIRARAGLKDERKPIGSFIFLGPTGVGKTELAKT
                     LARNLFDSEDNIIRIDMSEYMEKHAVSRLVGPPPGYVGYEEGGQLTEAVRRAPYSVIL
                     FDEIEKAHEDVFNMFLQILDDGRLTDNKGKTVDFKNTLIIMTSNIGSNYLLEAGGNIT
                     ETTNNLVMNEMKHRFKPEFLNRVDDIIMFKPLDQENIKKIIDIFMKDLKNRLKEKDIT
                     IEVTNSAKDVMVREGYDPVYGARPLKRYIGNTLETIIAKKLIAGDVYNGCTIVIDGKD
                     ENIEVLVK"
     gene            complement(5350..5946)
                     /locus_tag="PC414_003393"
     CDS             complement(5350..5946)
                     /locus_tag="PC414_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465763.1"
                     /translation="MSLKLDRNVLQWFDYVFENEKTSLRHYNFNCTLKEISSTSLNKV
                     AFILEKNNSKYWKLYFEIPAEVTLKLKQNIHPLFREYIYEQISLYNNNQIYNFVNSNI
                     LKVFNNIAIYQYNILENLYTIDFKKSFIDKCQYLLIGEKRLIDEDLYLIAKSKEVFDF
                     FNLDGTFNLTLSFDIQKNENLLDSLLELRKSIIINERI"
     gene            complement(5963..6367)
                     /locus_tag="PC414_003394"
     CDS             complement(5963..6367)
                     /locus_tag="PC414_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="HDF2465764.1"
                     /translation="MDKTLKKDLNQEYTESKSLIMNKSKYMNVCFKNYIKVVEGMYTK
                     RDLIPLKLINSMDKNNKIRKTLYELSCEFEYPKTSLSSLFTELKKMDFLQRVKNGEYM
                     INPHIAYKGSRADKESLLAEYNQIKRPNKVSK"
     gene            6734..7108
                     /locus_tag="PC414_003395"
     CDS             6734..7108
                     /locus_tag="PC414_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2465765.1"
                     /translation="MNIVISNSSGKPIYEQITTQIKSMIISGELPEGSALPSMRLLAK
                     ELRISVITTKRAYSDLERDGFIETVTGKGSFVSSKNMDFVKEEQFRLIEEYLQKAVDT
                     AKSSDITYDELKDILFLLYKGE"
     gene            7105..7968
                     /locus_tag="PC414_003396"
     CDS             7105..7968
                     /locus_tag="PC414_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2465766.1"
                     /translation="MISMDAITIKNLNKTYKDFSLQDISFSVPKGSVMGFVGENGAGK
                     TTTLKAILNLISYDSGNIEIFGLDSKKNEKEIKEQIGVVFEGSNFHENLNTDHVSKIM
                     SKIYKNWNDTLFKDYLKKLRVPDNKLIKEFSKGNKMKLSIAVALSHKPKLLILDEATS
                     SLDPIVREEILDIFLDFIQDEEHSIILSSHITSDLDKIADYITFIHKGKIVFSENKDE
                     LIDTMGVLKCKPSDFDNLSREDYSYYRKSQFGYEVLLKDKHRFISRHPNCIVDNTSIE
                     EIMLFYVRGDK"
     gene            7968..8606
                     /locus_tag="PC414_003397"
     CDS             7968..8606
                     /locus_tag="PC414_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HDF2465767.1"
                     /translation="MKGLILKDLLNLKGNIKFILLFIIMFGFMSSLGDGNVNNFIGVI
                     IVLCTTMIVSTFSYDDLNKWDSYVLTMPINRNDIVLSKYLTMLIFSFIGVLVSLIVSV
                     TIGYFKNTLILNETLLINALILSISVCFGSLILPLIYKFGTERARLLMILCFLVPTLA
                     LLVFKSILENISSPISIEIILNTLVYSLPFVAILLFVISYFISSKIYSKKEV"
BASE COUNT         2897 a         1271 c         1006 g         3456 t
ORIGIN      
        1 tattaagaaa aactcaaaga tttaaaaata tatattttct ataaagtata tttatgaatt
       61 atttcatatc ttcttttaaa ataacattac taacttcttt atgttcattt ctatacttag
      121 ttggagatat attataattc cttttaaata agcgactaaa ataatttatg ctatcaaagc
      181 cacatctcat agcaatttct gtcatattta aatctgtttc ctttaaataa tatatagctt
      241 tatcaagcct gattccattt ataaaatcga ttgtagtctt acctgttatt tgcttaaatg
      301 ttctacaaaa gtgatgtgca ctcaagttca catgactagc aagttctttt aatgtaattt
      361 tatccatata tttttcttct atcaaatcaa agatacattc aaatttttct aaagaatact
      421 ttctttttat aaattcatct tcagaatata ttttatcaat atgacttctt aataagagta
      481 ctattaattg atagatatta gcttttatag ataactcata tccaacttct ttattgtgat
      541 attcatcaag tatagttgta atacatttta aaatcagaga atcattttga attaaattat
      601 taaatacaat atgatttaaa gccaatggtg ctaagtactt ggtttgtaac aaatcaactt
      661 gattactaaa aaggaatgtg ggttcaatac gaatactata gaattttaaa ttatctgata
      721 agctttctaa ataatgtaat tcattactgt ttgctattac aatatttcca ggcactacct
      781 taaaattatc caatccacat tcaagatatg ctataccatc aataaaataa tatatttgta
      841 gatgctcatg ccaatgtctt ttaaagtttg ctcctttttt aatgtcatcc tttaaaaaaa
      901 tatctataaa gttttcttga cctttcctgt taaaatatgt atttttatcc ttcattattc
      961 ccccctcatc aaaatagtgc taattataag caatatagtt attgtattca ttatttcata
     1021 tcgctattat ataaaatgta actatcattg taaacattaa cataatgcag tcattataat
     1081 aaggaggtaa taaaaatgac aaactctttt aaaaaagatg ataatagaaa aaaaatcaca
     1141 gttttatttg tagtcattgc catgactttt atggcaacat tagattctag tattataaat
     1201 gttgctctcc cagttcttgc aagcaaacta aatgtatctc tagcatctat tgaatgggta
     1261 atagcaagtt actcaattat tatttgttca acactattat tttttggaag acttggagat
     1321 attattggta aatcaagagt atttcaagta ggtactattc tctttacatc agcttcatta
     1381 ctatgtggat taagtaattc tttaacttta cttatagttt gccgctttat tcaaggaata
     1441 ggcgcttctg cctacatggc aaacaatcat ggaattatca cagaactatt tccaaaagaa
     1501 agcagaggaa aagccctagg gattttagtt actgcagttg ctattggaaa tatggttgga
     1561 ccatctgtgg gaggatttat tttgtcaata tttgattgga atgttatatt ttttataaat
     1621 attccaatcg gtcttattgt aatcttttta aatacaaaat ttcttcctaa tagtaaaaaa
     1681 tcttcagaaa atatggataa aactggtgct atacttcaat ttttaggaac aacattattt
     1741 tttagtgctc taattagtgc tcaacaaaca ggattactaa atccatatat attaattgca
     1801 ctattattat caattatttt tattatatta tttttgatat tagaaaaaaa acatcctcaa
     1861 ccgcttcttg accttgaaat ttttagaaat tttaaatttt cattgaattt aatttgtgct
     1921 cttacatcat tcatttgtat tgcatcatca agtattttaa ttccatttta tttacaaagt
     1981 actatgaaac ttccccctat tcaagcaggt ttatttatga tactatcacc tttgatttta
     2041 gccatttttt cacctatatt tgggaatata tcagataaaa ttaagtcaga aaaaattatt
     2101 ttaattggtc ttctcgttat gagttttgga ttctttctaa tgtcaagatt gaaagaaagt
     2161 tctgccctta ttttatttgt aatttatata tcgataatat caataggaca agctatattt
     2221 caacctgcta acaatgcttt gataatgtca agctgttcta gaagtaagct aggagttgta
     2281 ggaagcataa attcccttgt aagaaattta ggacaagtta taggtattac tatttctaca
     2341 acacttttat ataattttat gagtattaaa gctggatata gggtcaatga ttatgtgatt
     2401 aataatgata aaatatttgt atttggaatg agaaatgttt atattattgt tacacttgtt
     2461 tgcttaatag gtgcaatatt aattggcttt tatttattta aatataataa aaatgaacaa
     2521 tagtttttat aattatattc aaattatgat tatattataa gaaatttact aaaatacttc
     2581 ctttgagtta aatttaattt ggacatattg acttaaaaat gttttattaa tttgataaaa
     2641 tatttctttt cctatagttt ataaataaaa atcagctgag caaaatattt agtatattat
     2701 gctcaactga ttcttttatt atttaactaa aacttcaata ttttcatctt ttccatctat
     2761 aactatagta catccattat acacatctcc agcaataagt ttctttgcaa ttatagtttc
     2821 taatgtattt cctatatatc tcttaagagg tcttgctcca tatacagggt catatccttc
     2881 tctcaccata acatcttttg ctgaatttgt aacttcaata gtaatatctt tttcttttaa
     2941 tctatttttt aaatctttca taaatatatc tataattttc ttgatatttt cttggtctaa
     3001 aggcttaaac attataatat catctactct gtttaaaaac tcaggtttaa atctatgctt
     3061 catttcattc attactaaat tgttagtagt ctctgttata tttcctcctg cttctaatag
     3121 ataattactt cctatattag aagtcattat tataagagta tttttaaagt ctactgtctt
     3181 acctttatta tcagtaagtc taccatcatc taatatctgt aagaacatgt taaatacatc
     3241 ctcatgtgct ttttctatct catcaaatag tattacagaa taaggagcac gtctaactgc
     3301 ttctgtaagt tgaccaccct cttcatatcc cacgtaccct ggaggaggtc caactagtct
     3361 tgatacagca tgtttttcca tgtattcaga catatctatt cttattatat tatcttcact
     3421 atcgaataaa tttctagcaa gagtttttgc aagttcagtt ttaccaacac ctgtagggcc
     3481 taagaaaata aatgaaccaa ttggttttct ttcatctttt aatcctgctc ttgcacgtat
     3541 tactgcattt gaaactgctg taactgcttc atcttgtcca ataactcttt tatgaagttc
     3601 atcttctagt ttaagtaatt tttctctttc tccttcaact agtttagtta ctggtatacc
     3661 tgtccacttt gatacaatgc tagatatttc ttcttcagta acttcttctt ttaaaagaga
     3721 attttcagaa ccatcattca tattttcttc gtactgcttt atttgttctt ctaacttagg
     3781 tatttcacca tatttaacct ctgctgcttt gttgaaatca tattctcttt catacttttc
     3841 agctttacct ttagcttcgt ctaattgagc ctttaagttt ttaatatcta atatttgact
     3901 cttttctttt tcatattttg ctgtcatttc atcattttta gattttaact ctgctatttc
     3961 tttttgaata tcatccaatc ttgatttact tttatcatca ttttctttaa gtaatgcttc
     4021 tctttcagtt tctaatgtaa ataactttct tctaacaaca tctaattctg ttggcaatga
     4081 atctatttca ctacgaatca tagctccagc ttcatcaatt aaatcaattg ctttatctgg
     4141 taaaaatcta tcttgtatat atctatgaga aagttttgct gctgctacaa tagcattatc
     4201 atgtattctt actccatgat gtatctcaaa tctttctttt aaacctctaa gtatagatat
     4261 tgtatcttca acagtaggtt cttctgcaat tacaggctga aaacgtcttt caagagcctt
     4321 atctttttct atatactttc tatattcatc aaaagtagtt gcaccaatac agttcaattc
     4381 acctcttgca agcataggtt taataagatt acctgcatcc attgaacctt ctgttttacc
     4441 agctccaaca atagtatgaa tctcatctat aaacagtata atttttccat cagaactttg
     4501 aacttctttt aaaactgcct ttaatctttc ttcaaattca cctctatatt ttgcaccagc
     4561 aattaaagct cccatatcaa gtgagaatac aatcttatct tttaaacctt ctgggacatc
     4621 tcctcttact attctctctg ctaacccttc tacaattgca gttttaccta cacctggctc
     4681 accaatcaat acaggattat tttttgttct tcttgataaa attcttataa tccttcttat
     4741 ttcttcatct ctcccaatta cagggtctaa tttatttttc ttggctaaat ctactaaatt
     4801 tgttccatat ctagcaagtg catcatatgt gccttctggg tcttgtgttt ctactctttg
     4861 actacctctc acttgtgata aaatatttaa aaagttattc ttatttatat tatattgttt
     4921 taatatttta ccaacagctg attttgattc tgtttccatc attgcaagca taacatgctc
     4981 tacacttata tatgaatctt taaattcttt agaaatactc tctgccttta taagcacctc
     5041 atttattttt ctagtagctg atactccttg tgagtcagca ccttctccat atacctttgg
     5101 aagcttatca atttcaaagt ttacagtatt tctcaaagaa tcaattgata tattcatttt
     5161 ttcgattata tttggtatca atccatcctc ttgattaata agtgctgaga aaagatgaat
     5221 tacatctact tgttggttat gattttttac tgcttcgtta taagcctcat ttaaactttt
     5281 ttgaactctt aaagtcattt tttctacgtc catataaatc cctccatttt aatattatat
     5341 ccaaataaat taaattctct catttataat tattgatttt ctaagctcta aaagactatc
     5401 aagtaaattc tcgttttttt gtatatcgaa actcaatgtt aaattaaatg ttccatctaa
     5461 attaaaaaaa tcgaatactt cttttgattt tgctattaaa tacaagtctt catctataag
     5521 tcttttttca cctattaata aatactgaca tttatctata aaactctttt taaaatcaat
     5581 agtgtataga ttttctaaaa tattatattg ataaatagca atattattaa acacttttaa
     5641 tatgtttgaa tttacaaaat tgtaaatttg attattgtta tacagtgata tttgttcgta
     5701 tatatattcc ctaaataaag gatgaatgtt ttgcttaagt tttaaagtaa cttcagctgg
     5761 tatttcaaaa taaagcttcc agtacttaga gttatttttt tctaaaataa aagctacttt
     5821 gttcaaactg gtagaactaa tctcctttag tgtacaatta aaattatagt gtctcaaact
     5881 agttttttca ttctcaaata catagtcaaa ccactgtaga acatttctat caagttttaa
     5941 actcatataa aaaccttctt ccttatttac taaccttatt aggtcttttt atctgattat
     6001 attctgctaa taaactttct ttatcagctc tactaccttt atatgctata tgtggattta
     6061 tcatatactc accatttttc actctttgta aaaaatccat ttttttcagc tccgtaaaca
     6121 atgaagataa agaagttttt ggatattcaa attcacaaga taattcatat aatgtttttc
     6181 taatcttatt atttttatcc atagagttta ttaattttaa tggaattaaa tccctcttag
     6241 tatacattcc ttctactact ttaatataat tcttaaaaca aacattcata tacttacttt
     6301 tattcataat taaacttttg ctctctgtat attcctgatt taaatccttt tttaatgtct
     6361 tatccaaatt aaatatctct tctcactctc cttatctcta attgataata taaatatatt
     6421 acaaaaaaat atgtatgtca aaactgaaaa tgcaaaaagt tcaccttata agtaaacttt
     6481 tatttagcta taagctaaac tttattattt ttttaattta gtatttccaa tttattggca
     6541 ggatttttga acaatattta taaatttttt aaaaaaattt aaatctattt aatatggcag
     6601 actttcataa aatacattac atttattaaa tgtataatat tggtaattag ttttttctaa
     6661 aatcagttga catattatat atatatttat ataatgtgta tatactatat atacaattta
     6721 aggaggtgtc ttattgaata tagttattag taattcgagt gggaagccaa tatatgaaca
     6781 gattactaca caaattaaaa gtatgattat aagtggagag ttaccagaag gctcagctct
     6841 tccaagtatg agactattgg caaaggaact tagaataagt gtaattacta caaaacgagc
     6901 atatagcgat ttagaacgag acggatttat tgagacagtt actggtaaag gcagttttgt
     6961 atctagtaaa aacatggatt ttgtcaaaga agaacaattt cgtttaattg aagaatacct
     7021 acaaaaagca gttgatacag caaaaagtag tgatattaca tatgacgaat taaaagatat
     7081 tttattttta ttatataaag gagaatgata agtatggatg caataactat aaaaaattta
     7141 aataaaactt ataaagattt tagcctacaa gatatatctt tttcagtacc aaaagggagt
     7201 gtaatgggat ttgtaggtga aaatggtgca ggaaaaacaa ctacattaaa agcaatttta
     7261 aatttaatat cttatgatag tggaaatata gaaatctttg gtcttgatag taaaaaaaat
     7321 gaaaaagaaa ttaaagagca aattggggtt gtatttgagg gaagcaactt tcatgaaaat
     7381 ttaaacactg accatgtttc aaaaatcatg agtaaaatct ataaaaattg gaatgacaca
     7441 ttatttaaag actacttaaa aaaattgaga gtaccagaca ataaattaat taaggagttt
     7501 tcaaagggga ataagatgaa acttagtatt gctgtagcct tatctcataa accaaaatta
     7561 ttaatactag atgaggcaac tagttcactt gaccctatcg ttcgtgaaga aatactagat
     7621 atatttttag attttataca agatgaagaa cactctatta tcttatcttc acatattaca
     7681 agtgatttag ataagatagc agactatatt acatttatac ataaaggaaa aattgtattt
     7741 agtgaaaata aggatgaatt aattgatact atgggagttt taaaatgtaa acctagtgat
     7801 tttgataacc tttcaagaga ggattattca tattatcgta aaagccaatt tggatatgaa
     7861 gtacttctta aagataagca tagatttatc tcaagacatc caaactgtat tgttgataat
     7921 acgtctatag aagaaattat gttattttat gtgagaggtg ataaataatg aaaggtctaa
     7981 ttttaaaaga tttacttaat ttgaaaggaa atataaagtt tatactttta ttcatcatta
     8041 tgtttggctt tatgtcttct ttaggagatg gaaatgttaa taactttata ggtgttatta
     8101 tagttttatg tactacaatg attgtttcaa cattttcata tgatgatttg aataaatggg
     8161 atagttatgt acttacaatg cctattaatc gcaatgatat tgtattaagt aaatatttaa
     8221 ctatgttaat atttagcttt ataggtgttc ttgtttcact tattgttagt gtaactattg
     8281 gttattttaa aaatacactt atattaaatg aaacactact tattaatgca ctaatcttat
     8341 caatttcagt atgttttggt agccttattc taccacttat ttataaattt ggtacagaaa
     8401 gagctcgttt attaatgatt ttatgctttt tagttccaac actagcttta ttagttttta
     8461 aaagtatttt agaaaatatt agttcaccta tttctataga aattatactt aatacactag
     8521 tatattctct tccatttgtt gctattttac tatttgtgat ttcatatttt atatcatcaa
     8581 aaatctatag taaaaaagaa gtataaaata tactatatat gtttctgtat
//
LOCUS       DAMJVK010000134         8621 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.139, whole genome shotgun sequence.
ACCESSION   DAMJVK010000134 DAMJVK010000000
VERSION     DAMJVK010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8621
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.139"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            260..1246
                     /locus_tag="PC414_003398"
     CDS             260..1246
                     /locus_tag="PC414_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDF2465768.1"
                     /translation="MKKITINDIANLAGVSKSTVSRYLNNKDISDSTKEKIKTIIDEY
                     GYEPNAFAQSLRAKKTYFIGIITPCLDSFVKSKIMMAIDEELKELKYTSLIINTNRKI
                     RSEIDSISKLASLKVDGIILIGTEITKEHKNEIEKLDIPIVVVGQKVDGINSIVNDDY
                     GAGYKMGQYIANKGYKNIVYLGVDESDISVGLNRKNGVLNGLKDKGYDAKVFYTDFDQ
                     ETSIQRSGEMLESENPDIIICATDNIAIATMKEINKRGKNIPQDISVAGFGGYDVLSI
                     ISPKLTTIKFENKNAGKVAANTIVNLIQERKEPLLKEIKFELIEGESTINKN"
     gene            1415..1563
                     /locus_tag="PC414_003399"
                     /pseudo
     CDS             1415..1563
                     /locus_tag="PC414_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423822.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
     gene            1582..3063
                     /locus_tag="PC414_003400"
     CDS             1582..3063
                     /locus_tag="PC414_003400"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HDF2465769.1"
                     /translation="MYKEPKYRTILESTKEELNKLRNISLNDKYKPLFHIHPQHGLLN
                     DPNGLAYYNGKYHVFYQWYPYDATHGMKHWAYVSSDDFVNWNREDVALIPIESYESHG
                     AYSGNAIEVDGKLHMYYTGNIKYSAEDRYAYQNLAIMNKDGKITKYENNPIVSEIPKG
                     YTGHVRDPKVFKRKDKYFMLLGAQTSDKKGAIIVYESKNSIDWNFKGELNVKNIDEDF
                     GYMWECPDYINIDEKDILIFSPQGVEPKGFDYQNIYNVVYAIGNMDLDNLTFEIDTMK
                     ELEKGFDFYAPQTFIKDSQIILFAWAGMGEVLYPTDKNKWAHCLTVPRKLNIKNNKLL
                     QMPVDELIKLRYDETSGQNTIKNNINIIENDENVYELNINIKNIDSNKFGLELFSSQD
                     EGVKLEFNKLGNIVTLDRSNFKKVFGVEYGTNRKEYINIDENTNIKVLADRSILEIFI
                     NEGEVVFTSRIFAKENSNQIRVYSDKIVEYEYTKFKLKQGIEL"
     gene            3089..4060
                     /locus_tag="PC414_003401"
     CDS             3089..4060
                     /locus_tag="PC414_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HDF2465770.1"
                     /translation="MKKVISIGEALIDFIPNQNGGKLKNVSEFRRVAGGAPANVSAVV
                     AKLGGKSSFISKLGKDAFGDYIIDVLNEVNVNTDYVLRTSKANTGLAFVSLKEDGNRD
                     FSFYRNPSADMLLEADEVKKEWFNNCHILHFCSVDLIDSPMKLAHKKAIEYALESNSI
                     ISFDPNIRLPLWDSEQSCKKAISEFLPFAHIVKISDEELEFITGENDIEKSLHKLFVG
                     NVELVLYTKGKDGVDAYTKKVKGMCKGVKVNAIDTTGAGDSYIGSFLYTLLYKQITLN
                     DIKEMKQETLNEYLEFSNYYAARSTTIKGAISSYATKEEITEFIKNL"
     gene            complement(4316..4918)
                     /locus_tag="PC414_003402"
     CDS             complement(4316..4918)
                     /locus_tag="PC414_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2465771.1"
                     /translation="MEVCILMGSPKKKGNTAAILKPFIEELENYNSNIEVIWLYDCKI
                     EPCIACKKCQDNLFDFGCYCKDDVQKIFKKILMCDLIVFATPIYSWYCTSPMKALLDR
                     LVYGMNKYYGNEKGPTLWAGKPVALITTCGYRPEKGADIWENGMKRYCKHSQLKYIGM
                     LVERDLGKPNFMNKEKEEHSRQFAKQIYYILDNSIEVDNC"
     gene            5199..6410
                     /locus_tag="PC414_003403"
     CDS             5199..6410
                     /locus_tag="PC414_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HDF2465772.1"
                     /translation="MYSNEDYKFIEENAPCYIYDETNIIEKCKELKNNILGFDFLYSI
                     KTNPYNKIVKSIAEQGFGADASSTNEVMISSECGVSKDDIYYSSPGKTEIDIETVLDK
                     CIIIADSITEIELINKISKKYNQKVKIGIRINPDFTMDDDSAKSSKFGIDIEDMSSVF
                     KMLSMCDYVSVVGIHIHIKSQVLDFEKLGHYYEKCFFIALNLNKSKYINIEFINFGSG
                     LGVAYDNLRENPLNLKELSKLTQKIAFTNSNTLKSRLLIESGRFIVCKSGNYYVKIVD
                     IKVSRGKKYLIVKNGLNGFMRPIISNLIENITCGVELEGQEPLYTSNHAFSLSVLNNL
                     KEEEYVNVVGNLCTSLDVIANNVKLKKANIGDLISISNAGSYSYSLSPLLFSSYEFPK
                     QYYKRKDGYIE"
     gene            6478..6675
                     /locus_tag="PC414_003404"
     CDS             6478..6675
                     /locus_tag="PC414_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HDF2465773.1"
                     /translation="MELWKRNLFVCWIGMFFSSIGMSQIAPILSLYIKQLGVTDVSLI
                     QQYSGIVFGCTFVVAAFFSPI"
     gene            6709..7680
                     /locus_tag="PC414_003405"
     CDS             6709..7680
                     /locus_tag="PC414_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2465774.1"
                     /translation="MLLRASIGMGIVIFTMAFVQNVYQLLGLRILQGVFTGYATACTT
                     LIATQTDKNHSGWALGTLATASTTGSLIGPTVGGYIESILGLKSTFIITGGLLFVSFI
                     ISILFVVDNFKPKEIKESISIPKEQLNILPNKFLIASIFVTTFITQLALYSIEPIVTI
                     YISQLTNFASNVALIAGLTFSASGLANLLAAQKLGKMSDKIGPQKVLLISLLWAGIIF
                     IPQAFVKTAWQLMLLRFLLGLSVAGLNPSVNSLLKKIAPEEYVGKIFGYNASAQYIGC
                     SSGAFLGGQISAHLGIRTVFFSTSLLLFMNALWMYKFTGLFIKDKTK"
     gene            7867..8244
                     /locus_tag="PC414_003406"
     CDS             7867..8244
                     /locus_tag="PC414_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2465775.1"
                     /translation="MTISKIPQAELKVMKFIWEKNDTVTSKETIEAMERKYGWKQTTT
                     LTLLSRLVKKHFLSAEKIDRYTHYTIVVGHKEYLSVETKDFLSNIHDNSLQSLISALH
                     DDEVVDKDMLDLFESHFKNLEEE"
BASE COUNT         3505 a          895 c         1368 g         2853 t
ORIGIN      
        1 ttgtaaaaat catagcctta tttttatatt ataaataaat tagagtattt atcaattcat
       61 agtagataaa attgaaatta aatataacaa caaaaaatga aaattacaat taatttaatg
      121 ttaaagactc aattatttgg atgtggaatg gatagtacaa gtagtatata aaaagtattg
      181 actggacaaa taactatatt ttattttaaa tatataatat aatgtaataa aatatatcaa
      241 atgtagaagg tgataaccta tgaaaaaaat aacaataaat gatattgcta acttggcagg
      301 agtatctaaa agtactgtat caagatatct aaataataaa gatatcagtg attctactaa
      361 agagaaaata aagacaataa tagatgaata tggatatgag ccgaatgcat ttgctcaaag
      421 tttgagagct aaaaaaacat actttattgg tattataaca ccgtgtttag attcttttgt
      481 aaaatctaaa attatgatgg caatagatga agaactcaaa gaattaaaat atacatcact
      541 aattattaat actaatagaa aaataaggtc agaaatagat agtatatcaa aattagctag
      601 tctaaaagtt gatggaataa tactaattgg taccgaaata actaaagaac ataagaatga
      661 aattgaaaag ttagatatac cgatagttgt agttggacaa aaagttgatg gtatcaatag
      721 catagtgaat gatgattatg gagcaggata taaaatgggt caatatattg ctaataaagg
      781 ttacaaaaac attgtatatt taggtgttga tgaaagtgat atatcagttg gtttaaatag
      841 aaaaaatgga gtacttaatg ggttaaaaga taaagggtat gatgcaaaag tattttatac
      901 tgactttgac caagaaacgt ctatacaaag aagtggagaa atgctagaaa gtgaaaatcc
      961 agatataata atatgtgcaa ctgataatat agcaatagca acaatgaaag aaatcaataa
     1021 gagaggtaaa aatataccac aagatatatc tgtagctgga tttgggggat atgatgtatt
     1081 gtctattata agccctaaac ttacaacaat aaagtttgaa aataaaaatg caggtaaagt
     1141 agcagcaaat acgatagtaa atttaataca agaaagaaaa gaaccactat taaaggagat
     1201 taaatttgaa ttaatagaag gtgaaagtac aataaataaa aattaagata cacattacat
     1261 atattagtaa tgtgtatttt tttactaaaa aaactataat tctaaaaatt aggaaaactt
     1321 ttttgaaaga aatattgcat ctgaaataaa tttatgctaa tctttaatta aaccggtttc
     1381 gaaaccggtt taattaaacc gatagaatag caaaatgagt gcaaaaataa ctgattgtaa
     1441 caaagtatca aagtaattat tagagtacat aggaataaac aaaatataaa agatgtagca
     1501 cattgtgtaa taagactaag aattgtttta gatagcaata ataaagcaaa tataaaatta
     1561 taaaagaaaa ggaataaaaa tatgtacaaa gagcctaaat atagaaccat attagaatcg
     1621 acaaaagaag aattaaataa attaagaaat atatctttaa atgataaata taaaccacta
     1681 tttcatatac atccacaaca tggactactt aatgacccaa atggattagc atattataat
     1741 ggtaagtatc atgtatttta tcaatggtat ccatatgatg caactcatgg aatgaagcat
     1801 tgggcttatg taagttctga tgattttgta aactggaata gagaagatgt agcattaata
     1861 ccaatcgaaa gttatgaatc tcatggagca tattcaggta atgcgataga ggtagatgga
     1921 aaattgcata tgtattatac agggaatata aaatatagtg cagaagatag atatgcatat
     1981 caaaacttag ctataatgaa caaggatggt aaaataacta aatatgaaaa taatccaata
     2041 gtaagtgaaa taccaaaagg atatacagga catgtaagag acccaaaagt atttaaaaga
     2101 aaggataaat actttatgtt acttggagca caaacaagtg ataaaaaagg agctataata
     2161 gtatatgaat ctaaaaactc tatagattgg aattttaaag gagaattaaa tgtaaaaaat
     2221 atagatgaag attttggtta catgtgggaa tgtccagatt atattaatat agatgaaaaa
     2281 gatatattaa tattttcacc acaaggagta gaacctaaag gatttgatta tcaaaatata
     2341 tataatgtag tatatgctat tggtaatatg gatttagata atttaacatt tgaaatagat
     2401 acaatgaaag aactggaaaa aggatttgat ttctatgctc cacaaacatt tataaaggac
     2461 tcacaaataa tactatttgc atgggctgga atgggagaag ttttataccc tacagataaa
     2521 aacaaatggg cacattgtct aactgttcca agaaaattaa atataaaaaa taataagcta
     2581 ttgcaaatgc cagtagatga attaattaaa ttaagatatg atgaaacaag tgggcaaaat
     2641 actataaaga ataatattaa tattatagaa aatgatgaaa atgtatatga gcttaatatt
     2701 aatataaaaa atattgacag caataaattt gggctggaat tattttcatc acaagatgaa
     2761 ggtgtaaaat tagaatttaa caaattagga aacattgtaa ctttagacag aagcaacttt
     2821 aaaaaagttt tcggtgttga atatggaaca aatagaaaag aatatataaa tattgatgaa
     2881 aatactaata tcaaagtact agcggataga agtattttag aaatttttat aaatgaagga
     2941 gaagtagtat ttactagtag aatatttgct aaagaaaatt ctaatcaaat aagggtttat
     3001 agtgataaaa tagtagagta tgagtatact aaatttaaat taaagcaagg tattgaatta
     3061 taaaaacaca ttataagggg tagaaaagat gaaaaaagta attagcatag gagaagcttt
     3121 aatagacttt ataccgaatc aaaatggtgg taaattaaaa aatgtatcag aatttagaag
     3181 agtagcagga ggagctccag caaatgttag tgctgttgta gcaaagcttg gaggaaaatc
     3241 gagttttata tctaagttag gaaaagatgc atttggtgat tatataatag atgtattaaa
     3301 tgaagtaaat gtaaatactg attatgtatt gagaacaagt aaagcaaata caggacttgc
     3361 ttttgtatca ttaaaagaag atggtaatcg tgatttctca ttttatagaa atccaagtgc
     3421 agatatgctt ttagaagctg atgaagttaa aaaagagtgg tttaataatt gtcatatcct
     3481 acatttttgt tcagtagatt taatagatag tccaatgaaa ttagcacata aaaaagcaat
     3541 agaatacgca cttgaaagta atagtataat aagctttgac cctaatataa gattaccttt
     3601 atgggatagt gagcaatctt gtaaaaaggc aatatcagaa tttttacctt ttgctcatat
     3661 tgttaagata tctgatgaag aattagaatt tataacagga gaaaatgata tagaaaaatc
     3721 attacataaa ctctttgtgg ggaatgttga actagtatta tatacaaaag gaaaagatgg
     3781 agtagatgca tacactaaaa aagtaaaagg tatgtgtaaa ggggttaaag taaatgcaat
     3841 agatacaacc ggagcaggag attcttatat aggttcattt ttatacacct tattgtataa
     3901 acaaataaca ttgaatgata taaaagaaat gaaacaagaa acgttaaatg agtatttaga
     3961 attttctaat tattatgcag ctcgtagtac aactattaaa ggtgcgataa gttcatatgc
     4021 aacaaaggaa gaaataactg aatttataaa aaatttatag taggaatgag taataggttt
     4081 tgaaggaagt aacatgtaaa gagtttttaa ggatgtttac tatgaaaata attaacatat
     4141 aaaaagcaat aaagaggctg tctcaaaata gagataagcc tctttatcat tgttgaaatg
     4201 ataaaaaata ttttaattat tgttactttt tagaagaata tatatcttat tttaaataag
     4261 caaattatgt aagatttatt tatattatca atactttact tggagtatta tgtctttagc
     4321 agttatctac ttcaattgaa ttatctaaaa tataataaat ttgttttgca aattgacgag
     4381 aatgttcttc tttttcttta ttcataaaat taggtttacc taaatcacgt tcaacaagca
     4441 ttcctatata ttttaactgt gaatgcttac agtagcgctt cataccattt tcccaaatat
     4501 cagcaccctt ttcaggtcta tatccacatg ttgtaataag tgctactggt tttcctgccc
     4561 atagagtggg gcctttctca ttaccatagt atttattcat tccatacacc aatctatcaa
     4621 gtagtgcttt cattggagaa gtgcagtacc aagaataaat tggagtagca aaaacaatta
     4681 aatcacacat aagaattttc ttgaaaattt tctgaacatc atctttacaa taacatccaa
     4741 aatcaaataa gttatcctga cattttttac aagcaataca tggctctatt ttacaatcat
     4801 ataaccatat tacttcaata tttgaattat aattttctaa ttcctcaata aaaggtttta
     4861 aaatagcagc agtattacct tttttcttag gacttcccat aagaatgcat acttccatat
     4921 tgatacctcc acagtatttt aaaatagttt ttagtgctta atatagtttt aggatactat
     4981 gagaatacgt taacaacaat acactttgta tatttgtaat aaaattgaaa caaattgttt
     5041 tatcaataaa agttggaatg tatctataat taaaaatatt ttaggtatgt ttttacatat
     5101 atgtgtgtag ttaactatgg taatttattg taaaattatc ttataggatt ataattgctt
     5161 aatttatgat tatatttaaa atcttggagg taaaaaatat gtactcaaat gaagactata
     5221 aatttataga agaaaatgca ccatgttata tatatgatga gactaatata attgaaaaat
     5281 gtaaggaatt gaaaaataat attttaggtt ttgatttttt atattctata aaaacaaatc
     5341 catataacaa aattgtaaaa agcattgcag agcaaggttt tggagcagat gcttcttcaa
     5401 ctaatgaagt tatgatttca tcagaatgtg gagtttctaa agatgatatt tattattcat
     5461 caccaggcaa aacagagatt gatatagaaa ctgttttaga taaatgcata attattgcag
     5521 atagtattac agaaatagaa cttataaata agatatctaa aaagtataat cagaaagtta
     5581 aaataggaat aagaataaat cctgatttta ctatggatga tgatagtgct aaatcaagca
     5641 aatttggtat agatatagaa gatatgagca gtgtttttaa gatgttaagt atgtgtgatt
     5701 atgtatctgt agttgggatt catatacata ttaaatctca ggtattggat tttgaaaagt
     5761 taggacatta ttatgaaaaa tgttttttta tagctttaaa tttaaataaa agtaagtata
     5821 ttaatattga atttataaat tttggtagtg gacttggagt agcttatgat aatcttagag
     5881 aaaatccact taatcttaaa gagctttcaa aattaactca aaagattgct tttactaatt
     5941 caaacacact aaaatcaaga cttcttattg aatcaggacg tttcatagtt tgtaaatcag
     6001 gtaattatta tgttaaaatt gtagatatta aagtatcaag agggaaaaaa tatcttattg
     6061 taaaaaatgg acttaatgga tttatgagac ctattatttc taatttaatt gaaaatatta
     6121 cttgtggagt agaattagaa ggtcaagagc cattatatac atcaaatcat gccttttctc
     6181 tttcagtatt aaataattta aaagaagaag agtatgtaaa tgttgttggg aatctctgta
     6241 catcattaga tgtaattgca aataatgtta aattgaaaaa agcaaacata ggagatttga
     6301 tttcaattag taatgctgga agttactcct attctttatc accgctatta ttttcaagtt
     6361 atgaatttcc taaacaatat tataaaagaa aagatggata tattgagtaa acaatagcat
     6421 aaattaataa acttagtatt attgaaaaaa tttaaaatgc agggggtgta attctatatg
     6481 gaattgtgga aaagaaattt atttgtatgt tggataggta tgttttttag ttctataggg
     6541 atgagccaaa tagcacctat actttcatta tatattaaac agttgggggt aactgatgtt
     6601 agtttaatac aacagtattc aggtatagtt tttggatgta cctttgtagt agcagcattc
     6661 ttttcgccaa tttgagggaa agctgctgat aaatatggaa gaaaatcaat gcttttacgt
     6721 gcaagtattg ggatgggaat agttatattt accatggcgt ttgttcaaaa tgtctatcag
     6781 ttattaggat taagaatact tcaaggagtt tttacgggtt atgctactgc atgtactact
     6841 cttattgcaa cacagacaga taaaaatcat tcaggatggg ctttgggtac tcttgcaact
     6901 gcatcaacta ctggctcatt aattggtcca acagtaggag gatatataga atcaatctta
     6961 ggtcttaaaa gtacatttat tataacagga ggattgttgt ttgtatcatt tatcatctct
     7021 attttatttg tagtagataa ttttaaacct aaagaaataa aagaatcaat ttctatacct
     7081 aaagaacaat taaatatatt gccaaataaa tttttaattg catctatttt tgtaaccaca
     7141 ttcattactc aactagcttt atactctata gaaccaattg taaccatata tatatcacaa
     7201 cttacaaatt ttgcatcaaa tgtagcttta atagctggat tgacattttc tgcatcagga
     7261 ttagcaaatt tattagcagc tcaaaaactt ggaaaaatgt cggataaaat aggtcctcaa
     7321 aaagtattgt taatttcact tttatgggct gggattatat ttataccaca agcttttgta
     7381 aaaactgcat ggcaactgat gttgttgaga tttttattag ggttgtcagt tgcagggctt
     7441 aatccttctg ttaacagctt gcttaaaaaa attgcaccag aagaatatgt gggaaaaata
     7501 tttggatata atgcatctgc ccagtatata ggctgttcat caggagcatt tttaggggga
     7561 caaatatcag cacatttagg tattagaaca gtattttttt caacaagttt attgttgttt
     7621 atgaatgcac tttggatgta taaatttact ggattgttta ttaaagataa gactaaataa
     7681 tcatactatt tacatttcta attttatgat gtaaaataaa tattttttat gtaatttatc
     7741 atagaattat ataaaaaata accctatggt aaaaattgga aaatattatt atcgacaaaa
     7801 tctttgactt aaaaactata ttgcgatagt attaatttaa taaaagtttg agaggggcgt
     7861 aaggttatga caatttcaaa aataccacaa gcagaattaa aagttatgaa gtttatttgg
     7921 gaaaaaaatg atacagtaac atcaaaagaa actattgaag ctatggaacg aaaatatggt
     7981 tggaaacaaa caactacatt gacactttta tcaagacttg ttaaaaaaca ttttttaagt
     8041 gcagaaaaaa tagatagata cactcattat acaatagttg ttggacataa ggagtattta
     8101 agtgttgaga caaaagattt tttaagcaat atacacgaca attctttaca gagtttaatt
     8161 tcagcactac atgatgatga agttgttgat aaagatatgt tggatttatt tgaaagtcat
     8221 tttaaaaatt tagaagaaga gtaattatat caggatagaa ctctaagaat ttaattaatc
     8281 tatggctgaa aaccataaat ggtattattt aatcagctac tcactattca taaagtaata
     8341 ttaaataggt tctctaatta gtaaaatgcc taaaataatt ggaataggga gtatcaagtt
     8401 aaatgatacc acttatttaa ataagaaatg atttttaaaa tttaaaataa ttttataact
     8461 tgtcaatatt cattaatcta aagtattatt tctatttaac catatcaaaa tatattttac
     8521 tatttttaat tattacaata taaacctgtc ttttgttatt ataggtaaac taaaagtaag
     8581 aaatgatttt tatattttta aactttatta ttggtaactt a
//
LOCUS       DAMJVK010000135         8561 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.140, whole genome shotgun sequence.
ACCESSION   DAMJVK010000135 DAMJVK010000000
VERSION     DAMJVK010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8561
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.140"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(72..542)
                     /locus_tag="PC414_003407"
     CDS             complement(72..542)
                     /locus_tag="PC414_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HDF2465776.1"
                     /translation="MKYQVSLLAVKDIEVSKKFYKELFEQEIELDLGKNVTFKGGFAI
                     QEDFAWLTGINPDTIIQKSNNMELYFEVDDFDEFIEKLNYYKDVEFVHKPLKHEWQQR
                     VVRIYDPDKHIIEIGESMEVIARRYLSEGYSVEETSKIIQHPIKFVKQCENSKE"
     gene            complement(1412..1801)
                     /locus_tag="PC414_003408"
     CDS             complement(1412..1801)
                     /locus_tag="PC414_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2465777.1"
                     /translation="MTIKKLPQSELKIMKFIWKSDSKVTSRDIVLGMEQKYQWKQTTT
                     LTLLSRLVVKRFLNSQKIDKYTHYEVLIKEKEYIGVETRDFFRNIHDSSIKSLLLSLH
                     ENINLSKDDILLIEEWIKNLKEEEKDV"
     gene            complement(3562..4968)
                     /locus_tag="PC414_003409"
     CDS             complement(3562..4968)
                     /locus_tag="PC414_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2465778.1"
                     /translation="MDSVKYILRRFIGSSILISLLFIVLNIAGFLLFSYMLFVSKYSD
                     NLDTSDSREIKSNLIMIEDNLILRDGKFILDDSSKEVLHKNKVWAMLIDNNGDRIWGF
                     DLPKEIPSHYTLTDVAKFSRFFLKDYPVYVWEHPNGILVTGYPKDKYTKFNLTYTVKE
                     IEQFPMLIIGMFLVNAIVTFLIALFIGLKMVKSIKPIITGIKLMSKGEPVLLQEKGIL
                     SDISKSINTVSKELQMKDEKLRKKEEARSNWIAGISHDIRTPLSMIIGYAGELEESSS
                     LSNREQEQVSIICNQGIKIRELVNDLNLVSKLEYNMQTLNCDKIRVSAFLRELISEFI
                     NNNLDNDFYVELDILNEDIIIDADKKLLKRAISNLIQNSITHNQQGCNIRVTSKSDLD
                     FCYIIVEDDGKGMSQERINSLLKSQNGLSEAYAKGQNHGLGILIVSGIINAHRGKLDI
                     TSIDSQGLKTILKLPLIK"
     gene            complement(5013..5720)
                     /locus_tag="PC414_003410"
     CDS             complement(5013..5720)
                     /locus_tag="PC414_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2465779.1"
                     /translation="MESIKDKKILVVDDHKELLKMIDEILRKEGFSRVFLASSYEEAV
                     RVFRNVKPDCAILDVVLPDGDGFSIMRKIRETSKIPVIFLSARGEDEDRLIGLGLGAD
                     DYIVKPFLPKELTLRLVGILNRVYVPIEEEELPVFKLGDSVVVNLNSACVEKDGQEIS
                     LTAKEHSLLLKLYENKGRIVTNDSLCQAIWGDDMYGYENTLMVHVRRVREKIEKTPST
                     PKHLITVRGLGYKLMIN"
     gene            5891..6805
                     /locus_tag="PC414_003411"
     CDS             5891..6805
                     /locus_tag="PC414_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2465780.1"
                     /translation="MEMIIRTQRLSKEYNKIFRVKDIDLRVPQGAVYGFLGPNGAGKS
                     TTLKMLLGLAKPTQGNINILGKELNEKNRISILKDIGSLIESPSYYGHLTGLENMTIM
                     QRLLDLPKKNVNEALKIVRLENQKNKKVSQYSLGMKQRLGIAMAIMKFPKLLILDEPT
                     NGLDPAGIEEIRELIKSLPEKYGMTVLISSHLLSEIDQIATSIGIINHGELVFQDSIK
                     ELHNRGQSQIAIKTKNVVSAQKLLNTKGYMSLIQDGYLTIENLKDSDVAKINGMLVHS
                     NIDVFRIEEHKKSLEDIFLELTGKAVTL"
     gene            6805..7566
                     /locus_tag="PC414_003412"
     CDS             6805..7566
                     /locus_tag="PC414_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2465781.1"
                     /translation="MKYLGLEFYKLKRKKIILMTFLFAAVEIMWCIVNSNRAITRNPD
                     MLGGFEYSYMLMSFASLNGLFFPILISIITSRISDIEHKGDTWKLLKSSVTSLNSIYL
                     SKFLCSAILVSIPVLMQVLSIVLFGHFRGVIESISVSLLLKYTLGTILVSIAIIALHL
                     WIATVIPNQMIAITFGMIGSFIGLTSGLFYQGIRRLFIWSYYLELSPLSYAYDNVLGS
                     SVYRIDMNFSITILVFLIGMLIYYIGRNHLLKKEV"
     gene            7578..8315
                     /locus_tag="PC414_003413"
     CDS             7578..8315
                     /locus_tag="PC414_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2465782.1"
                     /translation="MLRKSVLGEILKLKNSFIWYVLLALPLISVLIGSGNFYLNQGIL
                     KKEWYSLWTQVSLFYGEFFFPILIAIFCAYICRLEHMNHNWNNVMTLPVNLKNIFLSK
                     LTVVSILTAITQVFFVIFYIVAGNMFGFSGSVPKELTGWIAKGWIISISIASIQLYMS
                     IKIRSFATPIGISLCLSLFGMGLIVAKPAVGIFFPYSMLGSAMGVIKQSALNVNDTIV
                     TICVAFITTIVFSEIANRNFRKNDVIS"
BASE COUNT         2873 a         1394 c          873 g         3421 t
ORIGIN      
        1 ctagtcctat atattatctt gaattagttc tattacaatt aatttctact aaataattat
       61 aatttaaaca atcattcctt actattttca cactgcttta caaatttaat tggatgttga
      121 attattttag aagtttcttc tacagagtat ccctcagata aatatcttct agctataact
      181 tccatagact ctcctatctc aataatatgc ttatccgggt cataaatacg cactactctt
      241 tgttgccatt catgttttag tggcttatgg acaaactcta catctttata ataattgagt
      301 ttttcaataa attcgtcaaa atcatcaacc tcaaaataaa gttccatatt atttgatttt
      361 tgtataattg tatctggatt aatccctgta agccatgcga agtcttcttg tatagcaaat
      421 cctcctttaa atgttacatt tttgcctaag tccaattcaa tttcttgctc aaataattct
      481 ttataaaatt tttttgatac ttcaatatcc ttcactgcta ataatgaaac ctgatatttc
      541 ataaattttc ctcctaattt tttatttcta ttttttcagt atcttctatt aacatttttt
      601 tctctatctc ttcagtacct tttattaatc ctttattttt acttttttag catcttctat
      661 taacactttt gtttctacct attcagtatc ttatatcaat cttttatttt ttcaatatct
      721 tctattaaca cttttatctc tatctattca gtactttcta tcaatctttt gtttttatgt
      781 tttcaatatc ttctattaac acttttgttt ctacctcttc agtatcttat atcaatcttt
      841 tattttttca atatcttcta ttaacacttt tatctctatc tattcagtac tttctatcaa
      901 tcttttgttt ttattttttc aatatcttct attaatactt ttgtttctac ctcttcagta
      961 tcttatatca atcttttatt ttttcaatat cttctgttaa cacttttatc tctatctctt
     1021 taatatctta tattaatcct ttatttttat tttttcaata tcatcattaa ttcctattta
     1081 ttttatcatc ttaaatacga aaacactgtg tcatatttgc aaaatatata ttatcatcta
     1141 tattaaagct tcacaattta tatttaaaaa ttctctttaa ttacactacc tctatttaaa
     1201 aagctacctt aattatactt ataagtaaag gtagcttttt aaatttaatt aaatagaata
     1261 tccactatta atttatattt aatttattta gattcacatt atagtatcat aatccactta
     1321 taatcatttg tcatattact taagtagact aatatattac ataaatacac ttacatacac
     1381 tttatattga tgctataaga aatacgtttg cttatacgtc tttctcctct tcttttagat
     1441 tttttatcca ctcttcaata agtaaaatat catctttgct caaattaata ttttcatgta
     1501 atgacaacaa taaacttttt atagaagaat catgaatatt tctaaaaaaa tctcttgtct
     1561 caactcctat gtattccttt tctttaatta aaacttcata atgcgtatat ttatctatct
     1621 tttgagaatt tagaaatctt tttactacta atctagataa aagagtcaaa gttgtcgttt
     1681 gtttccattg atatttttgc tccattccta aaacaatatc tctagatgtt actttggaat
     1741 ctgatttcca tataaatttc attatcttta attctgattg tggcaacttt tttatagtca
     1801 tgataaaacc ttgctactca gaatatagac aagcaatatc cataataaaa tttttcgatt
     1861 aaatacatct tatacggatt tatataagct cagacttata agatattttt caatcttaat
     1921 cctaaattta gctatttgta cacggtaaat atatataaat aatgtcaaaa ttctcttgta
     1981 ttatattgct taattttatg aaatattttg tactcagcta tctattttgg atacttgtcc
     2041 agtagctctc ctttctttta aactctttga ttttaaatat ttgtatttca gtaaatccta
     2101 cttcctaaat tttttattta ctacaactta ttactgaact tcctacttcc taaacttttt
     2161 atttactaca acttattact gaacttctca cttactaaat ttcttactta ctataaatta
     2221 taactaaact tctcacttac taaatttctt acttactata aattactact aaacttccta
     2281 ctttctaaat ttcttacttg ctacaaatta ttactaaact tcctctccct aaatttctta
     2341 cttgctataa attattacta aacttcctac tttctaaatt tcttacttac tataaattat
     2401 tactaaactt cctactccct aattttctta cttactataa attattacta aatttcctac
     2461 tttctaaatt tcttacttac tacaaattat tactaaactt cctactccct aaatttctta
     2521 cttgctataa attattacta aacttcctac tccctaagtt tcttacttgc tacaaattat
     2581 tactaaactt cctacttcct aaatttctta cttgctataa attattacta ctaaacttcc
     2641 cacttcctaa atttcttact tactactact tataactaaa cttcccactt cctaaatttc
     2701 ttacttacta ccacttataa ctaaacttcc cacttcctaa atttcttact tactaccact
     2761 tataactaaa cttcccactt cctaaatttc ttacttacta ccacttatca ctaaacttcc
     2821 tactttctaa atttcttgct tcttaagaat aatgttaatt ttaattttaa gattatagtt
     2881 tttaccttgt ttaattgaaa cattctatat aattatatcc ttctatttta gagttttttt
     2941 atttatatat tacaaagttt aaattttttc attaattata ttataaattt tccatatatt
     3001 tcaacataaa tacatatata tatatgttat ttctaaaaat actacacaaa aactctactt
     3061 tagtagaact caatggcgca aaatacttca atttaaaaca tttatcgtac tttaaataaa
     3121 taactataat aaaatatact tacaatagtt acatagaagc tattataact acacagaaca
     3181 gcttataatt aaaacttttt taggatgtca ttatattttt tcttaaaatt aaaaaacact
     3241 ataccataat tctattttag tagaattttt ttgtcaataa taaaattaaa tattaactaa
     3301 aaaataaaaa aaatggaatt atctaaagaa tttaataacc ttacaataat tccaaaatat
     3361 tatttctatg aaaattttga taccatgagt aactcagcca taagcgcata aagcacaagt
     3421 tactctttac tttaataaaa cttgacaaga aaagaattta tccttattct actctaatag
     3481 agttaatttg tcaataaaga ataaaattat tttcaaaata ttttcattct tcattcttat
     3541 ctgttattat tttataaaat atcacttaat tagaggtaat tttagtattg tttttagtcc
     3601 ctgactatca atgctcgtaa tatctaattt acctctatgt gcattaataa tgccagatac
     3661 tatcaaaata cctagaccat gattttgacc tttggcatat gcctcactta gtccattttg
     3721 gctctttaat aaactattaa ttctttcttg actcatacct tttccatcat cttcaacaat
     3781 tatatagcaa aaatctaaat cagacttaga ggtaaccctt atattacatc cttgttggtt
     3841 atgagttata ctattttgta ttaaattact aatagccctt tttaataatt ttttatctgc
     3901 atctataata atatcttcat ttagtatatc aagttcaacg taaaagtcat tatccaaatt
     3961 attatttata aactcagaaa taagctctct caaaaacgca gaaactctaa tcttatcaca
     4021 attcaatgtt tgcatattat attcaagttt agacactaaa tttaggtcat ttactaattc
     4081 tctgatttta atcccttgat tgcatataat agacacttgc tcttgttctc tatttgaaag
     4141 gctactgctt tcttcaagtt ctccagcata tccaattatc attgaaagtg gagttcttat
     4201 atcatgagat attcctgcaa tccagttaga cctagcttcc tctttcttcc taagtttttc
     4261 atctttcatt tgcaactctt tagatacagt attaatagat tttgaaatat cacttagtat
     4321 tcctttttct tgtaaaagta ctggttctcc ttttgacata agctttatgc cagtaataat
     4381 tggttttata gatttcacca tttttaaacc tatgaataga gctattaaaa aagtaactat
     4441 tgcattaacc agaaacatac ctattattaa cataggaaat tgttctattt ccttaaccgt
     4501 ataagtaagg ttaaacttag tatacttgtc ttttggataa cctgttacta atattccatt
     4561 tggatgttcc catacataca caggatagtc ctttaaaaag aaccttgaaa actttgctac
     4621 atctgtcaaa gtataatgac ttggaatttc ttttggtaaa tcaaatcccc atattctatc
     4681 tccattgtta tcaattaaca ttgcccaaac tttattttta tgtagtactt ccttactaga
     4741 atcatctaaa ataaactttc catctcttaa aatcaaatta tcttcaatca taatcaagtt
     4801 tgattttatt tctctactat cacttgtatc taaattatca gagtatttag atacaaataa
     4861 catatatgaa aacagaagaa aacctgctat atttaataca ataaaaagta atgaaatcaa
     4921 gatacttgaa cctataaatc gcctcaatat gtactttaca ctatccaaaa ttaaatcact
     4981 cctcttgatt tcttttattc taatatctaa aattaattaa tcatcaactt atatccaaga
     5041 cctcttactg taataagatg tttaggtgta gatggagttt tttctatctt ctctctaacc
     5101 cttctaacat gaaccataag ggtattttca tatccataca tatcatctcc ccaaatggct
     5161 tgacatagtg aatcattagt aacaattctc cctttatttt catacaactt taaaagcaac
     5221 gaatgctctt tagctgttaa agaaatctct tgcccatctt tctcaacaca tgcactattt
     5281 aaattcacaa caacagaatc tcccagttta aatactggca actcctcttc ttctataggc
     5341 acatatacac gatttaatat acccacaagc cttaaagtaa gttcctttgg taaaaatggc
     5401 tttactatat aatcgtcagc ccctaagccc aaaccaatca atctatcttc atcttcacct
     5461 cttgcagaga gaaaaatcac tggtatttta gatgtctctc taatttttct cataatagaa
     5521 aaaccatctc catctggcaa tacaacatct aaaattgcac aatctggctt tacatttcta
     5581 aataccctaa cagcttcctc ataacttgat gctaaaaata ctctactaaa tccctctttt
     5641 ctcaggattt catctatcat ttttaataat tccttgtggt catcaaccac aagtattttt
     5701 ttatctttta ttgactccat aatcatcacc ctcctttagt ataccatata cccattttca
     5761 caactttgcc taaatagact tttaaggtta ttgtaaggta aatttcagct aattgaaatg
     5821 taaccaagtt attatactga tataagttaa aagaatcaat aagcacagat taaaaaaggg
     5881 gatgaaacat atggaaatga taattagaac acaaagactt tcaaaggaat acaataaaat
     5941 atttagagta aaggatatag acctaagagt tccacaagga gctgtatatg gttttttggg
     6001 tcctaatgga gctggaaaat ctaccactct taaaatgctt cttggacttg ctaaacccac
     6061 acaaggaaat attaatattt taggtaaaga gttaaatgaa aaaaatagaa tatcaatttt
     6121 aaaagatata ggttctttga tagaatctcc atcatactat ggtcatctta cagggcttga
     6181 aaacatgacc ataatgcaaa gactcttaga tttacctaaa aaaaatgtaa atgaagcatt
     6241 aaaaattgta aggttagaaa atcaaaaaaa taaaaaagtt agtcaatatt ctttaggtat
     6301 gaagcaacgt cttggcattg ctatggcaat aatgaagttt cccaaattat taatacttga
     6361 tgaacctaca aatggactcg acccagcagg gattgaggaa attagagaat taataaaatc
     6421 tctaccagaa aaatacggga tgactgttct aatctctagc catcttttaa gtgaaattga
     6481 ccaaattgcc acatcaatag gaataataaa tcatggcgaa ctggtttttc aagatagcat
     6541 aaaagaactt cataatagag gtcaaagtca gattgctatc aaaactaaga atgttgtatc
     6601 tgcacaaaaa ctattaaata ctaaaggata tatgtcactt atacaagatg ggtacttgac
     6661 tattgaaaat ttaaaagact cagatgtcgc taaaataaat ggtatgcttg tccattcaaa
     6721 tatagatgta tttagaatag aagaacataa aaaaagcttg gaagatatat tcttagaact
     6781 tactggtaag gcggtgactt tataatgaaa taccttggtc tagaatttta taaacttaaa
     6841 agaaagaaga ttattctgat gaccttttta tttgcagctg ttgaaataat gtggtgtatc
     6901 gtaaattcaa atagagctat tacacgtaat ccagatatgt taggtggatt tgaatattcc
     6961 tatatgctta tgagttttgc aagtttaaat ggtctattct ttcctatctt aatatcaata
     7021 attacatcac gtataagcga tatagaacat aaaggagata catggaagct tttaaaatct
     7081 tctgtgacat ctttaaatag tatttattta tctaaatttt tatgttctgc tattttggta
     7141 tctatacctg tattgatgca agtgttatct attgtgttgt ttggacattt tagaggggtt
     7201 attgagtcaa tttctgtatc tttactttta aaatacacat taggaacaat tcttgtaagt
     7261 attgctatca ttgcactaca cctatggatt gcaactgtaa ttccaaatca aatgattgca
     7321 attacatttg gcatgattgg tagttttata ggtttgacat caggtttgtt ttatcaaggt
     7381 attcgtagat tgtttatatg gtcttattac ctagagttat cacctttaag ttatgcttat
     7441 gataatgttt taggttcatc tgtctataga attgatatga atttttctat tacgatttta
     7501 gttttcttaa ttggaatgct aatttattat attggaagaa atcatttatt aaaaaaagaa
     7561 gtataggagg gatttaaatg ttaaggaagt cagttttggg agaaattcta aagttaaaaa
     7621 attcttttat atggtatgta ttattagctt tgccacttat aagtgtgttg attggttctg
     7681 gtaattttta tctaaatcaa ggcatactca aaaaagaatg gtacagctta tggactcaag
     7741 ttagtctttt ttatggggaa tttttctttc ctatcttaat cgcaatcttt tgtgcatata
     7801 tttgtaggct tgaacatatg aatcataatt ggaataatgt aatgacactg ccagttaatt
     7861 taaaaaatat ctttttatct aaacttacag tagtttcaat acttacagct attacacaag
     7921 ttttctttgt tatcttttat atagttgcag gaaatatgtt tggtttttct ggtagtgtac
     7981 ctaaagaatt gactggttgg attgctaaag gttggattat ttctatttca atagcttcta
     8041 tacaattata catgtctata aagattagaa gttttgcaac tcctattggt attagtcttt
     8101 gcctttctct atttggtatg ggattaatag tagcaaaacc tgctgttggt atatttttcc
     8161 cttattctat gttaggtagt gctatggggg ttataaagca atctgcttta aatgttaatg
     8221 acactatagt tacaatatgt gttgcattta ttactactat agtattttct gaaattgcca
     8281 atagaaattt tagaaagaat gatgttattt cttaatggta aatgctagat atttttgggt
     8341 ttgaaattta tattcttttt agttgaaatc tatatccatt ttatttaaaa atatatatct
     8401 tttatttgaa ttttatatgt tttatctact cgctatataa tagatatact cctaaaattt
     8461 aaaaatatat aataatgtta ttatttaaaa ttattatgta ttatattaaa acttaatgca
     8521 aaaacaattt ttatattata taatgaaact taattaaata t
//
LOCUS       DAMJVK010000136         8276 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.141, whole genome shotgun sequence.
ACCESSION   DAMJVK010000136 DAMJVK010000000
VERSION     DAMJVK010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8276
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.141"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(131..655)
                     /locus_tag="PC414_003414"
     CDS             complement(131..655)
                     /locus_tag="PC414_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF2465783.1"
                     /translation="MENKNPIVTIEMENGKEIKIELYPNIAPNTVKNFVSLVNEGYYN
                     GIIFHRVIPGFMIQGGCPNGTGMGGPGHSIKGEFSGNGFTNNLKHERGVISMARTMAP
                     NSAGSQFFIMHKNSPHLDGQYAGFGRVIEGMDTVDEIASVRTDSADKPQTPQVMKSVT
                     VETFGIDYSDVEKN"
     gene            complement(720..1472)
                     /locus_tag="PC414_003415"
     CDS             complement(720..1472)
                     /locus_tag="PC414_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDF2465784.1"
                     /translation="MKLGLCLEGGGAKGAYQAGVVKALYDGGINKFYSISGTSIGAIN
                     GYYLYTGNVDNLEKMWTNIKDIQNGNVKIVNNTVDNSPAIDNLRELDDSNIEEMNFYV
                     NYVEVDNKVVSEKIVDVSKMPRNEAIISISYSGLLPSNPNATLGFKEQFVKDVQEGIY
                     DGFKLDGGLIRSALIEPLIGDNVDKIILISTKYNYELPEDIKKVYDEDKIIVVRPNTQ
                     FAPKDTLNFDDEFCKTIYQEGYEIGKNILDRL"
     gene            complement(1503..2801)
                     /locus_tag="PC414_003416"
     CDS             complement(1503..2801)
                     /locus_tag="PC414_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HDF2465785.1"
                     /translation="MDSINFAKNLIDFIYDSPSSFHAVESTKEILDKNGFEELVLNQR
                     WNLRVGGKYYVTKNLSAIVAFVVNSEGIERDGFRIIGSHSDSPTFSIKPNAEIEAEKS
                     YLKLNTECYGGAILSTWLDRPLGIAGRVVLKGDSILKPNERLIDFKKPICIIPNLAIH
                     LNRSINDGHSYNKQKDMLPLVGMLNDTLEKDNFLIKQIASNLDVNMDDIIDFDLFLYE
                     FEKGSLIGANDEFISIGRQDNLAMVHASLNALVNTKGQHGVNVMAVFDNEEVGSSTKQ
                     GADSNMLLNILERICISMGKDREGFFESIYSSFMISSDLAHAIHPNIPEKHDPTNKPI
                     MGKGPVIKINASQAYTSDGYSIAIYKNICREANVEYQEFVNRSDERGGSTIGPISSTH
                     IDIPSVDVGSPILAMHSIRELGNVEDHYSIYKTFSKFFEI"
     gene            complement(2818..5082)
                     /locus_tag="PC414_003417"
     CDS             complement(2818..5082)
                     /locus_tag="PC414_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HDF2465786.1"
                     /translation="MNLDTLNPAQREAVEKTEGPVLILAGAGSGKTKVLTTRIAYLIE
                     DKGVQAPNILAITFTNKAANEMRERVEQNIGPETKDMWISTFHSCCVRILRKDINKIG
                     YNRSFVIYDSADQVTLVKDCLKELNLSDKVFEPKAVISAISGAKDKLYTPKQFKDINM
                     ADNRMVKIADIYALYQDRLKRNSALDFDDLILKTVELFKANDEVLAYYRSRFRYIMVD
                     EYQDTSKAQYELIKLLAREHQNICVVGDDDQSIYGWRGADIRNILEFEKDYDNVHVVK
                     LEQNYRSTQVILDAANKVISNNIERKRKKLWSEKKEGELIKIQLTGSEIEEADFIADS
                     IAQIARKENRPYKDFAVLYRANAQARPVEDALNRSQIPYNIYGGTKFYERKEIKDLLA
                     YLRVIQNPQDDISIKRIINVPRRGIGLRTIEKIEDRANLKQESIYSVLIDIETNSDIS
                     TKARASISGFVDIIGTLRTIKEVYPVSKLIEKVLDTTGYMDELVEIRNKNEKDLTGKG
                     EEAQDRIDNLREFISIALEFESSNDDTYENKDLETFLTSIALTSESNDEEDNDRVSLM
                     TIHTSKGLEFPVVFLTGMEEGLFPISRAIKSMSDSQIEEERRLCYVGITRAKEELYMS
                     LTEKRTLYGKTNVAIASRFMEELPEECIERLYKVKKELSYSKASYNMLDKYTKKYMST
                     ISKSKVADKVNATIKDSNKETNPDDIKLGSKVHHPKFGVGTVVSIIGTDVTIAFDQQG
                     IKKINKEYTTLNIL"
     gene            complement(5337..6155)
                     /locus_tag="PC414_003418"
     CDS             complement(5337..6155)
                     /locus_tag="PC414_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDF2465787.1"
                     /translation="MFKINNLTYQYEKNTNALLNINMDFSKGNVIGIIGSNGSGKSTL
                     FMNLMGILKPTSGEILFKEEKLKYDKRSLYNLRKNVGIVFQDPEKQIFYSKVYDDIAF
                     AMRNIGMDEKTIKERINKALVAVNGIDFIDRPVHFLSYGQKKRVAIASVIAMENEIVL
                     LDEPTAGLDPVSTRSIVDIIKGLNKNNIKIVISSHDMNLMYEICDYIYVLDKGILIDE
                     GKAENVFINENNIIQAGLESPWLVKVHRNMNLPLFKKEEDLYKYWKERELNTNK"
     gene            complement(6148..6846)
                     /gene="cbiQ"
                     /locus_tag="PC414_003419"
     CDS             complement(6148..6846)
                     /gene="cbiQ"
                     /locus_tag="PC414_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HDF2465788.1"
                     /translation="MLIIDKYAYTNRLSKVNPNKKVAIGVIFLIASMVIQNIFILSGI
                     MILMSILVVCVAGIDLKNYLKLLRIPMYFLFLSIGITLVNISFNKADLLYSFEFFSFN
                     VGISKASINMSIHVLFRAMSCLTCVYFCILTTPFNQLIFFFKKLHLPDTFVELSMLIY
                     RFIFIFLEEFSEIYKSQELRFGYINLKTSYKSLGILGSMLYKRLMTRYDDMCISLDIK
                     LYDGKFHIVGDNDV"
     gene            complement(6849..7133)
                     /locus_tag="PC414_003420"
     CDS             complement(6849..7133)
                     /locus_tag="PC414_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2465789.1"
                     /translation="MSAKTKTKTNILLLLLVVALIIFPLLVNSGAEYGGADGQAESEI
                     TKINPNYKPWFSSPYEPPSGEIESLLFSAQAALGAGVIGYILGLQKGKRS"
     gene            complement(7123..7875)
                     /locus_tag="PC414_003421"
     CDS             complement(7123..7875)
                     /locus_tag="PC414_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HDF2465790.1"
                     /translation="MKQNIKLGVIAALMLIILTPVTSNAMHIMEGYLPVKWSIAWGVI
                     FIPFFLFGLKSIGKIVKQDPKKKVLLALCGAFVFVLSALKIPSVTGSCSHPTGVGLGA
                     IMFGPSVMFVLGTIVLIFQALLLAHGGITTLGANAFSMAIIGPIVSFLIFKALKKKDG
                     NNAIPVFLAAAIGDLATYTITSIQLALAFPDPSGGVMASAIKFLGIFFMTQIPIAIAE
                     GILTVIVYNLITENGEKSILENNDKGVKANEC"
     regulatory      complement(8057..8249)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
BASE COUNT         2594 a         1507 c          898 g         3277 t
ORIGIN      
        1 acaatacaaa taaattaaaa agtgaaatgt atttgtctaa aaacataata acatttcact
       61 ttatcctcat ttttaagtaa tatatatttt taatttttat ttataactaa atctagtttt
      121 tatataaatt ttagtttttt tctacatctg agtaatctat tccaaaagtt tctacagtaa
      181 cagatttcat aacttgtgga gtttgtggct tatcagctga atctgttcta actgatgcta
      241 tttcatcaac tgtatccata ccttctatca ctctaccaaa acctgcatac tgaccatcta
      301 agtgtggtga gtttttatgc attatgaaaa attgagaacc agctgaatta ggtgccatag
      361 ttctagccat tgatatgact cctctttcat gttttaaatt gttagtaaat ccattaccac
      421 taaattcacc ttttattgag tgacctggac cacccattcc tgtaccattt gggcatcctc
      481 cttgtatcat aaatcctggt attactctat gaaatattat tccattgtaa tatccttcat
      541 ttactaatga tacaaagttt ttaactgtat ttggagctat atttggatat aactcaattt
      601 tgatttcttt tccattttcc atttctatag ttactatagg atttttattt tccataattt
      661 attaccttcc ttttcattag ttaaattaca taattacatt tattatatta tactctaaat
      721 taaagtctat caagtatatt ttttcctatt tcatatcctt cttgataaat agttttacaa
      781 aactcatcat caaagttaag cgtgtcttta ggagcaaatt gagtattagg tctaactact
      841 attattttat cttcatcata tactttttta atatcttctg gcaactcata attatatttt
      901 gtggatatta aaataatttt atccacatta tccccaatta atggttcaat taatgcactc
      961 ctaattaaac ctccatctaa tttgaaacca tcataaattc cttcttgcac atctttcaca
     1021 aattgttctt taaatccaag agttgcgttt ggatttgatg gtaacaaccc actgtatgaa
     1081 atgctgataa tggcttcatt acgaggcatt tttgatacgt caacaatctt ttctgatact
     1141 actttattat caacttcaac ataattaaca taaaagttca tttcttctat attactatca
     1201 tctagttccc ttaggttgtc tatagcagga gaattatcca cagtattatt tactattttt
     1261 acatttccat tctggatatc ttttatattt gtccacattt tctccaagtt atcaacattc
     1321 cctgtgtaca aataatatcc atttattgct cctattgatg tccctgaaat cgaataaaat
     1381 ttatttattc ctccatcata caaggcttta actactcctg cttgataagc cccttttgct
     1441 cctccacctt ctaaacataa tcctaatttc atagtctcat ctccaaattg tttatcttat
     1501 atttatattt caaaaaactt actaaatgtt ttatatatac tatagtggtc ctcaacattt
     1561 cctaattctc ttatagaatg cattgctaaa attggacttc ccacatccac agatggtata
     1621 tctatatgag tagaagatat tggacctata gtacttcctc ctctttcatc tgacctatta
     1681 acaaattctt gatactcaac attagcttct ctacatatat ttttataaat agctatagaa
     1741 tatccatcac tagtatatgc ctgactagca tttatcttta taaccggacc ttttcccatt
     1801 attggtttat tagttgggtc atgcttttct ggaatgtttg gatgaatagc atgagctaaa
     1861 tctgatgata tcataaaaga tgaataaata gactcaaaga atccctctct atccttaccc
     1921 attgatatgc aaattctctc caatatattt aacaacatgt ttgaatctgc accttgttta
     1981 gtagagctac caacttcttc attgtcaaat acagccatta catttacacc atgttgacct
     2041 ttagtattaa caagagcatt caaactagca tgtaccattg caagattatc ttgtctccca
     2101 atagatataa attcatcatt agcccctatt aaacttcctt tttcaaattc atataaaaat
     2161 aaatcaaaat ctataatatc gtccatattt acatctaagt tagatgctat ttgctttata
     2221 agaaaattat ctttttctaa tgtatcattt aacatcccaa ctaaaggtaa catatctttt
     2281 tgcttattat atgagtgacc atcatttata cttctattta aatgtattgc tagatttggt
     2341 attatacaaa ttggcttttt aaagtctata agcctttcat ttggttttaa tatactatca
     2401 ccttttaaaa ctactctacc agctatccca agaggtctat ccaaccatgt acttaatatt
     2461 gctcctccat agcattctgt atttaacttc aaataagatt tttcagcttc tatttctgca
     2521 tttggtttaa tactaaatgt aggagaatct gaatgagagc ctattattct aaatccatct
     2581 ctttctatac cttctgaatt tactacaaaa gctactatag ctgataaatt cttagtaaca
     2641 taatactttc caccaactct tagattccat ctttgattca aaactaattc ttcaaatcca
     2701 tttttatcta agatttcctt agtactctcg actgcatgaa aagacgacgg actatcatat
     2761 atgaagtcta ttaagttttt agcaaagttt atactatcca ttcctcattt acctccatta
     2821 taaaatattt agtgttgtat attctttatt tattttttta attccctgtt ggtcaaaagc
     2881 tatagtaaca tctgtaccaa ttatagaaac cactgtccca accccaaatt taggatgatg
     2941 taccttagaa ccaagtttta tatcatctgg attagtttct ttattagaat cttttattgt
     3001 agcattaact ttatctgcaa ctttagattt gcttatagta ctcatatact ttttagtata
     3061 tttatctaac atattataac ttgctttaga atagcttagt tcttttttta ctttgtacaa
     3121 tctttctata cattcttctg gcagttcttc cataaatctt gaagctattg caacatttgt
     3181 tttaccatat aatgttctct tctctgtcaa tgacatatat agctcttctt tagctctagt
     3241 tataccaaca taacaaagtc ttctctcttc ttctatttgt gaatcactca tagattttat
     3301 agctcttgat ataggaaata acccttcttc catacctgtt aaaaacacaa cagggaactc
     3361 taaacctttg gatgtatgaa ttgtcataag cgaaacccta tcattgtctt cttcatcatt
     3421 tgattcagat gtaagtgcta tacttgttag gaatgtctct aagtctttat tttcataagt
     3481 atcatcatta ctactttcaa actccaaagc tatagaaata aattctctca aattatcaat
     3541 tctgtcttga gcttcttcac ctttgcctgt taaatctttt tcatttttat ttcttatctc
     3601 tacaagctcg tccatatatc ctgtagtatc caagactttt tctataagtt tgctaacagg
     3661 ataaacttct tttatagttc taagagtccc tataatatca acaaatccac ttatacttgc
     3721 ccttgcctta gtagatatat ctgaatttgt ttctatatca attaatactg agtatatact
     3781 ttcctgtttt aagttagctc tatcttcaat tttttctata gttctaagcc ctatacctct
     3841 tctaggaaca tttataatcc tttttataga tatatcatct tgtggatttt gaataactct
     3901 taaatacgcc aacaaatcct ttatttcttt tctctcatag aattttgtac caccatatat
     3961 attgtatgga atttgacttc tatttaaagc gtcctctaca ggtcttgcct gtgcatttgc
     4021 tctataaagt actgcaaaat ccttataagg tctattttct tttctagcta tttgtgcaat
     4081 tgaatcagca atgaaatcag cctcttctat ttcactacca gttaattgta tctttataag
     4141 ttctccttct tttttttcac tccaaagctt ttttcttttt ctttctatat tattagatat
     4201 aactttatta gctgcatcta atataacttg tgtagaccta taattttgtt ccaactttac
     4261 aacatgtaca ttgtcataat ctttctcaaa ttcaagtatg tttcttatat ctgcacctct
     4321 ccatccataa atactttggt catcatctcc aacaacacaa atattttgat gttctcttgc
     4381 taataatttt atcaattcat attgagcttt acttgtatct tggtactcat ctaccattat
     4441 gtatctaaat ctacttctgt aatatgctaa cacttcatca ttagccttga aaagttctac
     4501 agttttaagt attaaatcat caaagtcaag tgcactattt ctttttaaac ggtcttgata
     4561 tagcgcataa atatctgcaa tcttaaccat tctattatct gccatattga tatctttaaa
     4621 ctgctttgga gtataaagtt tgtcttttgc tcctgaaatt gcacttataa ctgcttttgg
     4681 ttcaaacact ttatcactta aatttaattc ttttagacag tctttaacca atgtaacttg
     4741 gtctgctgaa tcataaatta caaaacttct attgtaacca atcttattta tatcttttct
     4801 tagtatacga acacagcaag aatggaatgt acttatccac atatccttag tttcaggacc
     4861 tatattttgt tcaactctct ctctcatttc atttgcagct ttgtttgtaa atgttatagc
     4921 taatatattt ggtgcttgta cacctttatc ttctataaga tatgctattc ttgttgttaa
     4981 taccttagtt ttacctgaac cagcaccagc cagtataagt actggacctt cagtcttttc
     5041 tactgcttct ctttgagcag gatttaatgt atctaaattc atatttctcc tcctgttaac
     5101 agccataaat atattttaat ctaaaatatg aattacttca aattataatt atgtattata
     5161 ttaatagatt atttccaata tattacaatt aaataacatt tttccatata atttatttca
     5221 ttgtttaata aaaaaaataa ccttatgatt tggggaatca taaggcacct ttaaggggtc
     5281 taggttaagg gttattatat ataaatctat ttttttaaaa taggtttagg gcttaattat
     5341 ttatttgtat taagttctct ttccttccaa tacttataca aatcttcttc tttcttaaat
     5401 aagggtaaat tcatatttct atgtactttt actaaccatg gggattctag gccagcttgt
     5461 ataatattat tttcatttat aaacacattt tctgcttttc cttcatcaat caaaatcccc
     5521 ttatccaata catatatgta atcacatatt tcatacatta aattcatatc atgactggat
     5581 attactattt ttatattatt tttatttaat ccctttatta tatctacaat agacctagta
     5641 cttactgggt ctaaaccagc tgtaggttca tcaagtaata caatttcatt ttccattgct
     5701 ataactgatg ctattgccac tctttttttc tgtccatagc taagaaaatg tactggcctg
     5761 tctataaaat ctattccatt aacagctact agagctttgt ttattctctc tttaattgtt
     5821 ttttcatcca taccaatgtt tctcattgca aaggctatat catcatatac ttttgaataa
     5881 aaaatttgtt tttctgggtc ttggaagaca atacctacat tttttctaag gttgtataaa
     5941 cttctcttat catattttaa cttttcttct ttaaaaagaa tttctccaga tgtaggcttt
     6001 aatataccca ttaaattcat aaataatgtt gattttcctg aaccatttga acctattata
     6061 cctataacat ttccctttga aaaatccata ttaatgttta atagtgcatt ggtatttttt
     6121 tcatattgat acgttaaatt atttatttta aacatcatta tcccctacta tgtgaaattt
     6181 accatcatac aattttatgt ctaatgatat acacatatca tcataccttg tcataagtct
     6241 tttgtacagc atacttccaa gaatacctaa ggatttatat gaagttttta aatttatata
     6301 tccaaatctt agctcttgtg acttgtatat ttctgaaaat tcttctaaaa atatgaatat
     6361 aaacctatat attaacattg ataactcgac aaaagtgtca ggtaaatgaa gttttttaaa
     6421 aaagaatatt aattgattaa aaggtgttgt aagtatacaa aaatacacac aagtcaaaca
     6481 agacatcgct ctaaataaca catgaataga catgttaata gacgcctttg atattcctac
     6541 attaaaacta aaaaattcaa aactatataa taaatctgct ttattaaaag atatatttac
     6601 aagagttatt cctatactaa gaaataaaaa gtacattggt attctaagta attttaaata
     6661 attttttaaa tctattccag ccacacaaac aaccaaaata ctcattaaaa tcataattcc
     6721 acttaaaata aatatatttt gtattaccat agaagcaatc agaaaaatta ctcctatggc
     6781 tacttttttg tttggattta ctttactcaa tctatttgta tatgcatatt tatcaattat
     6841 aagcatgatt aacttctctt tcctttttga agacctaata tgtatcctat tacaccagcc
     6901 cctaaagctg cttgtgcaga gaataataaa ctttctattt ctccacttgg tggctcatat
     6961 ggactgctaa accatggttt gtaatttgga tttatcttag taatttcact ttcagcttga
     7021 ccatctgctc ctccatattc agcacctgag ttgacaagta atgggaatat aataagagct
     7081 actactaata aaagtaataa tatgttagtt tttgtttttg ttttagcact cattagcttt
     7141 aactccttta tcattatttt ctagtatgct tttttctccg ttttctgtaa ttaaattata
     7201 tacaattaca gtaagtatac cttctgctat tgctattggt atttgagtca taaagaaaat
     7261 tcctaaaaat ttaattgctg aagccattac cccacctgat gggtctggga aagccagtgc
     7321 taattgtatt gaagttattg tatatgtggc taagtctcct attgctgctg caagaaatac
     7381 tggaatagca ttatttccat cttttttctt taaagcctta aatattagga atgatacgat
     7441 tggccctatt atcgccattg aaaaagcatt agctcctaaa gtagttattc ctccatgtgc
     7501 taataacaat gcttggaata ttaaaactat agttccaaga acaaacatta cgcttggtcc
     7561 aaacattatt gctccaagac ctacaccagt aggatgtgaa caacttcctg ttactgaagg
     7621 tatttttagt gctgataata caaacacaaa tgctccacat aaagctaata atactttctt
     7681 tttagggtct tgctttacta ttttacctat tgattttaat ccgaataaga aaaatggtat
     7741 aaatataact ccccatgcta tagaccattt tactggtaag taaccttcca ttatatgcat
     7801 tgcatttgat gtaactggag ttaaaatgat taacataagt gcagctataa cccctaattt
     7861 aatattctgt ttcatcttag tatccctctc tccctttcta ataattaaaa cataataata
     7921 catgtctgat ttatttaata aaataaaaaa gcctaactcg atggttaggc ttctaatttt
     7981 ccacaatcta aatacaaaca cataaaacaa acatatctgt ataattatta taagaaactt
     8041 tctccatccc cgaagaaatt actttaatat tttaggcagg tctcctgact tagcttcatc
     8101 ctactccttt tccttcccat gagatataaa aaatatcatc atagtggcta acaaaagttt
     8161 cgtctgcatc acagtagcgg gggctgtaaa ggaatttaac ctttttccct attaatcttg
     8221 cttaaagaac ctataatatt atatatttaa tttttaaatt attcttaaat catttt
//
LOCUS       DAMJVK010000137         8148 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.142, whole genome shotgun sequence.
ACCESSION   DAMJVK010000137 DAMJVK010000000
VERSION     DAMJVK010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8148
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.142"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            <1..597
                     /locus_tag="PC414_003422"
     CDS             <1..597
                     /locus_tag="PC414_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2465791.1"
                     /translation="GLIKESSGQVLLNKHNLSNLAEHRRTQIVSRVFQDPSLGTCPSM
                     TVRENLSLALNKGKLLNLRKCLRYKRDFLENLLEGVSLDLKKYLDVQVKFLSGGQRQS
                     LSLIMSCLTNPSVLLLDEHTAALDPKTSNEVIELTNKIVREKNITTLMVTHNLKHALH
                     YGDRLIMLHKGEVVLDVKGKEKEQLTVEEILEKFEYAV"
     gene            728..1750
                     /locus_tag="PC414_003423"
     CDS             728..1750
                     /locus_tag="PC414_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2465792.1"
                     /translation="MINKKRLASLILAGALSISMLTGCSQGGDSGNSKQESNSKGKEV
                     KKIGITQLVEHPALDATRTGFVKALEKNGFKDGENIDIDFQNAQNDMPTTQSIASKFA
                     SDKKDLIFAISTPSAQAAFNATKDIPILITAVSDPVAAGLVKTLEKPGTNVSGTSDFV
                     SVDKGLELLKIFAPKAKTIGVMYNTSEVNSKVQVDALKEYASKNGFKVVEKGITTSNE
                     VNQGISSLVGKIDVLYVPTDNLVASSMPIVSKIATENKIPVIAAESGPVEKGALACQG
                     INYEKLGYKTGEMAVKILNGESVSDMPVATSDDTDIIVNEDILKALGMEKPSNENISY
                     VKTKQE"
     gene            complement(1914..2471)
                     /locus_tag="PC414_003424"
     CDS             complement(1914..2471)
                     /locus_tag="PC414_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDF2465793.1"
                     /translation="MTEKEKMLSGKGYYANDELLVKEREYCKKLTRLFNNTLEDEYEK
                     REDILRQLFGSVGKQINVEQNIRCDYGYNIHVGENFFANYDCIFLDVCKIEIGDNVML
                     APNVQIYTAYHPIDAQLRNSGIEYGSPIKIGDNVWIGGGVIITPGITIGDNVVIGAGS
                     VVTKDIPPNTVAVGNPCRVIKKIEE"
     gene            complement(2477..3553)
                     /locus_tag="PC414_003425"
     CDS             complement(2477..3553)
                     /locus_tag="PC414_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HDF2465794.1"
                     /translation="MSLYVDIEKDLSSFKLKVEIKQEKGTLGFLGESGSGKSMTLKCI
                     AGLEKPTRGKIVLNDRVLFDSEKKINLSTQDRKVGFLFQNYALFPHMTVSQNIELGLL
                     KLSKSEKKEIVARYLDILKLNGFEGRYPWQLSGGQQQRVALARALATSPDILLLDEPF
                     SALDHHLRSNMEKELMNMLKDYKGDILFVTHDIEEAYRVCDDIIVYNKGEGLPKRPKK
                     ELFESPKYLIEAKITGCKNISKLNRLDKNTIYATDWGCELTLNREIGDNIEYVGIREH
                     HIKVLDSNEDLNEKLCFELINIVENPFTYTIYVRKTDLSNECVPIQIELEKSKMRFKK
                     GDRIYLDFPQEYLFCFRYNYNKKE"
     gene            complement(3554..4237)
                     /gene="modB"
                     /locus_tag="PC414_003426"
     CDS             complement(3554..4237)
                     /gene="modB"
                     /locus_tag="PC414_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HDF2465795.1"
                     /translation="MGTDWSPLWISLKTSILSTIITFVIGIFVSYIMANYRGKWKGLI
                     DGLFTLPLILPPTVVGFFLLLLCGKNGFIGKFLLNFDRTLIFSWTATVISAVVVSFPM
                     MYRTSRSAFEQIDNNMLSAARTLGLNEWKVFFKIAVPLAWPGIIGGLVLSFARALGEF
                     GATLMIAGNIPGKTQTMPIAIFFAVEGGDMNKAMMWVMIIVAISIAMILLLNYWSEFQ
                     QKIIGKRCG"
     gene            complement(4386..5198)
                     /gene="modA"
                     /locus_tag="PC414_003427"
     CDS             complement(4386..5198)
                     /gene="modA"
                     /locus_tag="PC414_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2465796.1"
                     /translation="MKKILGILGLVACLTLGTVGCNSNEDKKDDGKQEKTTKSSDSVE
                     LNISAAASLKEAMAKIEEEYKKVDSNVKLTVNYGASGSLQQQIEQGAPCDLFISAGQK
                     QMKALDEEKLLVSDTMKDLVKNDLVLISSADSSVSGMKDLTTDKVKKIAVGEAESVPA
                     GKYADEVLTNLNLKDKLKDKLVFAKDVKEVLAWVQSGNADVGFVYFSDTVNNDKIKVV
                     EKTDEKTHSPITYPVSVIKASKNVDAAKKFEEFLLSESGQKIFEEFGYKKVE"
     gene            5391..6320
                     /locus_tag="PC414_003428"
     CDS             5391..6320
                     /locus_tag="PC414_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2465797.1"
                     /translation="MDSLSSLTPVEVAELLKITKNTVYELIKRGELPSYKVGKKIRVD
                     TKDVEEYINSQKTGKVKDTHFNNKNIDINNNFKIIISGQDVILDILARSVEKKLDGIS
                     TFRSYIGSYNALYELYNGRVSIASSHLWDFETDEYNSTFVKKLLPGIPCVLINLAYRM
                     QGFYVAKGNPKEITTWEDLTKPSITIVNREKGSGTRVLLDGKLRLLNFNGKHIKGYDN
                     EELSHLGVASTVSRGIADVGLGNEKAALQVNNIDFIPLHKERYDLVIKKEDLQNPVYQ
                     TIINIINSPEFKAELQGLGGYDLTDTGKTIAKV"
     gene            complement(6481..6930)
                     /locus_tag="PC414_003429"
     CDS             complement(6481..6930)
                     /locus_tag="PC414_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465798.1"
                     /translation="MKNKNNKDKNMLNIKVLRVLGVLLILFDYTGDTLNFYNFFAKPL
                     VSGNLYKTTGTDCIVFLLFIVECVYLVGRYILKVKINWSEIIENEKGNFINNLLVEKI
                     QEESNIVYNLLNSYVLPNIYKKARGLMFKTYHMLIYIKEKYYYVNIY"
     gene            complement(7133..8113)
                     /locus_tag="PC414_003430"
     CDS             complement(7133..8113)
                     /locus_tag="PC414_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2465799.1"
                     /translation="MKNSFKNCISLRNKFIIISIVFLLILLIVSYIFGKLVYNSSVGL
                     EQLIKNEDIVKIFSERKDRPLEKLKNYKTSELMIQSSNNYKLESLFITSNIKTRDTMI
                     LVHGIGSSYYEMLKVAYRYLDKGYNVLVYNQRNTGNSGGDNYTFGLYERYDLDSLVKF
                     VKNKFPEGRLGVHGFSMGAGTAAMHSEINSKDDKVDFYILDSPYSEMKDAIRMGVLEK
                     RIPDILINYVVTCGDLYNKFKSGFWYSDVKPYESVEKSNVPILFIHGTKDTVCNYQNS
                     KKMYDLVKHDKKDLWLIEGIGHVDGFEHDSTVYFNKIFKFIDSNVLSDKS"
BASE COUNT         2815 a         1347 c          979 g         3007 t
ORIGIN      
        1 gggcttataa aagaatcttc tggtcaagta ttattaaata aacataactt gtctaattta
       61 gctgaacaca gaagaactca aatagtaagt agagtttttc aagacccaag tcttggaact
      121 tgcccttcta tgactgttag agaaaacttg tctttagctc ttaacaaagg aaaactatta
      181 aatctaagaa aatgtcttcg ttacaaaaga gatttcttag aaaatttatt ggaaggtgta
      241 tctcttgatt taaaaaaata ccttgatgtt caagtaaaat ttttatcagg aggtcaaaga
      301 caatcattat cactaattat gtcgtgctta acaaatccat cagttttatt acttgatgaa
      361 catacagcag cacttgaccc aaaaacatca aatgaagtaa ttgaattgac taataagatt
      421 gtaagggaaa aaaatataac tacacttatg gttactcaca acttaaaaca cgctcttcat
      481 tatggtgata gattgataat gcttcataag ggtgaagttg tattagatgt aaaaggtaaa
      541 gaaaaagaac aattaacagt tgaagaaatc ttagaaaaat ttgaatatgc agtttaaaat
      601 aatatatcta ttgtattaaa ctcggcttta aaataaagtt aaaattacaa taattatggt
      661 tactttgtaa taattcatat gaatatatga acatatataa caataaattt ttgaggggga
      721 tataaaaatg attaataaaa aaagattggc aagtttgatt ttagctggtg ctcttagtat
      781 atctatgctt acagggtgtt cacaaggagg agactctggt aattcaaaac aagaaagtaa
      841 ttctaaaggt aaagaagtta aaaaaattgg tataactcaa ctggttgaac atccagcatt
      901 agatgccact aggacaggtt ttgtaaaagc attagaaaag aacggattta aagatggaga
      961 aaatattgat atagacttcc aaaatgctca aaatgacatg cctactacac aaagtattgc
     1021 gagtaaattt gcatctgata agaaggactt aatatttgct atatctactc catcagctca
     1081 ggcagcattt aatgctacta aagatatacc tatacttatt actgctgtga gtgaccctgt
     1141 tgctgctgga ttagttaaaa ctcttgaaaa gccaggcaca aatgtatctg gaacatcaga
     1201 ttttgtttct gtagacaaag gtttagaact tcttaaaata tttgctccta aggcaaaaac
     1261 tataggagtt atgtacaata ctagtgaagt aaattccaaa gttcaagttg atgctttaaa
     1321 agaatatgca tctaaaaatg gatttaaagt tgttgaaaaa ggtataacta cttcaaatga
     1381 agttaaccaa ggtatttcta gtttagttgg caaaatagac gtattatatg tccctacaga
     1441 taacttagtg gcttcttcta tgcctatagt ctctaaaata gctactgaga ataaaatacc
     1501 tgttatagca gctgaatctg gtccagttga aaaaggtgct cttgcttgtc aaggtatcaa
     1561 ctacgaaaaa ctaggttaca aaacaggtga aatggcagtt aaaattttaa atggcgaatc
     1621 agtatctgat atgcctgtgg ctacatcaga tgatacagat atcattgtta atgaagatat
     1681 attaaaagct ttaggtatgg aaaaaccttc taatgaaaat atttcttatg taaagactaa
     1741 acaagaatag aaagatattt tctatatacg attaatatgc ctgtttctat aaattaattt
     1801 atagaaacag gcatatattt ttattttaat gtattatcta tttaaagatt atatgtaaat
     1861 gctcttaatt tgtttgtata aattttactt gtataaactt tatacaatca tatttattct
     1921 tctattttct ttattactct acatggattt ccaacagcta cagtatttgg tggtatatct
     1981 tttgtgacta cacttcctgc acctattact acattatcac ctatagtaat tcctggtgtt
     2041 atgattactc ccccacctat ccatacatta tcacctattt ttataggaga accatactct
     2101 atgcctgaat ttctaagttg tgcatcaatt ggatgatagg ctgtatagat ttgaacgttt
     2161 ggagctaaca ttacattgtc acctatttca attttacata catctaaaaa aatacaatca
     2221 taatttgcaa agaaattttc gcctacatgt atgttatatc cataatcaca cctaatattt
     2281 tgttctacat ttatttgctt gccaactgaa ccaaatagtt gtcttaaaat atcttctctt
     2341 ttttcatatt catcctctaa agtattatta aacaatctag ttaatttttt acaatactct
     2401 ctttctttta ctaacaattc atcatttgca tagtatcctt tcccagacaa cattttttct
     2461 ttttcagtca tatagtttac tcctttttgt tataattgta tctaaagcaa aataaatatt
     2521 cctgtggaaa atctaaatat attctgtcac ctttcttaaa tctcatttta cttttttcca
     2581 attctatttg aataggtaca cattcgttag ataaatcagt ttttctaaca tatatagtat
     2641 atgtaaaagg attttctaca atgttaatca attcgaaaca taacttttca tttaaatctt
     2701 cattagaatc caagacctta atatgatgct ccctgattcc cacatattct atattatcac
     2761 ctatttctct atttaatgtc aactcgcatc cccagtctgt agcataaata gtatttttat
     2821 ccagtctatt taatttggat atatttttac accctgttat cttagcttct ataaggtact
     2881 ttggagattc aaagagctct tttttaggtc tctttggaag accttctcct ttgttgtaca
     2941 ctataatatc atcacaaact cggtaagctt cttcaatatc atgagtaaca aatagtatat
     3001 ctcccttgta atcttttaac atattcataa gttctttttc catattgctt cttaaatgat
     3061 ggtccaatgc tgagaaaggt tcatctaaaa gtaatatatc tggagatgtt gccaatgctc
     3121 tagctagtgc taccctttgt tgttgaccac cagataattg ccaagggtat ctcccctcaa
     3181 acccatttaa tttaagtata tctaagtacc ttgctactat ttcctttttt tcactcttac
     3241 ttagttttaa aagcccaagt tctatatttt gacttacagt catatgtgga aacaaagcat
     3301 agttttgaaa taaaaatcct acctttctat cttgtgttga taaatttatc ttcttttcag
     3361 aatcaaatag aactctatca tttaatacaa ttttacctct tgttggtttc tcaagccctg
     3421 ctatacattt taatgtcata ctttttccag aaccagattc tcccaaaaac cccagagtac
     3481 ctttttcttg tttaatttct acctttagtt taaaagagga taaatctttt tcaatatcca
     3541 cgtataagct caactatcca cacctctttc ctataatttt ctgctgaaat tccgaccaat
     3601 aatttagtaa taaaatcatt gctatagata ttgctacaat aatcataacc cacatcatag
     3661 ctttattcat atctccgcct tcaacagcaa aaaatatagc tattggcata gtttgagttt
     3721 ttccaggtat atttccagct atcataagtg tagcaccaaa ttctccaaga gctctggcaa
     3781 acgacagcac caatccacca attattccag gccatgctaa tggaactgca atcttaaaaa
     3841 atactttcca ttcattaaga cctaatgttc tagcagctga caacatatta ttatcaatct
     3901 gttcaaaagc tgaccttgaa gttctataca tcataggaaa agacaccact actgctgaaa
     3961 ttacagttgc tgtccaacta aatattaaag tcctatcgaa atttaataaa aactttccta
     4021 taaatccatt cttcccacat agtaataata aaaagaaacc tacaactgtt ggtggcaaaa
     4081 ttagtggaag tgtaaatagt ccatctataa gacccttcca cttacctcta tagttagcca
     4141 taatatatga cacaaatatt cctattacaa aagttattat tgttgacaaa atagatgtct
     4201 taagtgatat ccataagggt gaccaatctg ttcccatctt tccctcctta attttaaatg
     4261 gaaatagtga aactaataac tctattaatt tcactatttc cattttatta taatacacaa
     4321 ttattttcaa ttttcattta tttatatata atttatactt aattacacat aatttatttc
     4381 taagtttatt ctactttttt atatccaaat tcttcaaaaa tcttttgtcc tgactcactt
     4441 aataaaaatt cttcaaattt cttagcagca tctacgtttt tactagcttt aattactgaa
     4501 actggatatg taataggcga atgagttttt tcgtctgttt tttcaactac ttttatctta
     4561 tcattattga cagtatcact gaagtaaacg aatccaacat cagcatttcc tgattgcacc
     4621 catgctaaaa cttcttttac atcttttgca aatactagct tatcttttaa cttatctttt
     4681 aaatttaaat tagttaacac ctcatctgca tacttaccag ctggaacact ttcagcttca
     4741 ccaacagcaa ttttcttcac tttatctgta gtcaaatctt tcatacctga tacactactg
     4801 tcagcagaag aaataagaac taaatcattt tttactaaat ctttcatagt atcagaaact
     4861 aataacttct cttcatccaa agctttcatt tgtttttgac ctgctgaaat aaataaatca
     4921 caaggtgctc cttgttctat ttgttgttgt aatgagcctg atgctccata gttaactgta
     4981 agttttacat ttgaatcaac ttttttatat tcttcttcaa tctttgccat tgcttctttt
     5041 aaacttgcag cagccgatat atttaactct actgaatcac tacttttagt tgttttttct
     5101 tgttttccat catctttttt gtcttcatta ctattgcacc caactgtccc caaagttaaa
     5161 catgcaacta atcctaatat tcccaatatt tttttcataa caatctccct ttttgtaaat
     5221 ttgatacaca ttttaacatg ttttattatg ttttatcatg ttttgttata tatagtttaa
     5281 ttatatatac atgataataa attttcaagt aatttattta cactactttt aaatattttg
     5341 ctacattttc ctttttttaa tgttaaaata accttatatg gaggtggatt atggactcat
     5401 tatcatcttt gacacctgtc gaggtagctg aattattaaa gataacaaaa aatactgtat
     5461 atgaattaat caaaagaggt gaacttcctt cttataaagt agggaaaaaa ataagagttg
     5521 atactaaaga tgttgaagaa tatatcaaca gccaaaaaac tggtaaagta aaggatacac
     5581 atttcaacaa taaaaatata gatataaata ataatttcaa aattattatt tctggtcaag
     5641 atgtaatatt agacatttta gctaggtctg ttgaaaaaaa attggatgga ataagtacat
     5701 ttcgctctta tataggtagt tataatgctt tgtatgagtt atataatggc agagtttcta
     5761 tagcatcatc ccatctatgg gatttcgaaa cggatgaata taatagcact tttgttaaaa
     5821 agctattgcc aggaatacca tgtgtactta taaatctagc ttatagaatg caaggtttct
     5881 atgttgctaa aggaaatcct aaagagataa caacctggga ggatttaaca aaaccaagta
     5941 taaccattgt aaatagggaa aaaggttctg gaactagggt tttactagat ggtaaactta
     6001 gacttctaaa ctttaatggt aaacatataa aaggttatga taatgaagaa ttatcacatc
     6061 ttggagttgc aagtactgtt tctcgtggaa tagcagatgt tggtttggga aatgaaaaag
     6121 ctgctttaca agtaaataat atcgacttta ttcctcttca caaagagcgc tatgatttag
     6181 ttattaaaaa agaagactta caaaatccag tttaccaaac tataattaac ataataaact
     6241 ctcctgagtt taaagctgaa cttcaaggtc ttggcggata tgatttaact gatactggaa
     6301 aaactatagc aaaagtttaa gattaaaatc gcaatctatt ttataagatt tataattttt
     6361 cacaaaattt ataacttaaa cacgaaataa attgcgattt tagtgtctca aaaaatttat
     6421 gtctttcatt ttatcatcca atacaaaata taatataatt aattgtgcaa tcatattata
     6481 ttaatatata tttacataat agtatttttc tttaatgtag attaacatat gatatgtttt
     6541 aaacatcaat ccccttgctt tcttatatat atttggcaac acatatgagt ttaataaatt
     6601 atatacaatg ttgctttctt cttgtatttt ttcaaccaac aagttattta taaaattacc
     6661 tttctcattt tcaataatct cactccaatt tatcttaact tttaatatat atcttcccac
     6721 taggtataca cactctacaa taaataataa aaatacaatg cagtcagtac ctgttgtctt
     6781 atataaatta cccgatacta atggctttgc gaagaaatta taaaaattca aagtatcacc
     6841 cgtataatca aaaagtatta atagcactcc tagaactctc aatactttaa tattcaacat
     6901 gtttttatct ttattatttt tgtttttcat atatactacc tctcttttct ttagatttga
     6961 ttgtaatcta atactaactc ttttatttga ggtataaatg aatttaattg gtagttattt
     7021 tcatttttac tagaaattaa ttcacattac taaacaaaac ttatatttaa acataaaaat
     7081 agcctatcta ttttttaata aatacaaaaa taaataagct actaatattc aattaacttt
     7141 tatcacttaa aacattacta tctataaatt taaatatttt attgaagtat actgtactat
     7201 catgttcaaa tccatcaaca tgaccaatac cttctataag ccataggtct tttttatcat
     7261 gtttaactaa atcatacatt ttcttgctat tttgataatt acaaacagta tcttttgttc
     7321 catgtataaa cagtatgggt acattagatt tctcaactga ctcatatggt tttacatcac
     7381 tgtaccaaaa tccagattta aacttattat ataaatctcc acaagtcacc acataattga
     7441 ttaatatatc tggtattcgt ttttctagta ctcccattct aattgcatct ttcatttcag
     7501 aataaggaga atccagtata taaaaatcga ccttatcatc ttttgaattt atctctgaat
     7561 gcatagcagc tgtccctgcc cccatggaaa atccatgcac tcctaatctt ccttcaggaa
     7621 atttattttt tacaaattta actagactat ctaaatcata cctttcataa agaccaaatg
     7681 tgtaattatc tccaccacta ttaccagtat ttctttgatt ataaactaat acgttataac
     7741 ctttatctaa atatctatat gctaccttta acatttcata gtaactactt ccaattccat
     7801 gaacaagtat catagtatct cttgtcttta tatttgaagt tataaaaaga ctttcaagct
     7861 tgtaattatt agaactttgt atcattaatt cagatgtctt gtagtttttt aatttctcca
     7921 gtggtctatc ttttctctca gaaaatattt ttactatatc ttcgtttttt attaactgct
     7981 ctaaaccaac tgaactatta tacactaatt tgccaaatat ataagataca attaatagga
     8041 ttaatagaaa cacaattgat ataattataa atttatttct taaactaata caatttttaa
     8101 aactattttt catatttatt taccatacaa ttttcctata ttatattt
//
LOCUS       DAMJVK010000138         7890 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.143, whole genome shotgun sequence.
ACCESSION   DAMJVK010000138 DAMJVK010000000
VERSION     DAMJVK010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7890
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.143"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     misc_binding    109..320
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            483..1772
                     /locus_tag="PC414_003431"
     CDS             483..1772
                     /locus_tag="PC414_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HDF2465800.1"
                     /translation="MEEEKINYALEQIPENQKRGWVAMFSVLVAIGVDLSSVILGAEL
                     AQSMPMKQAILSVIVGSFFSAILYTTCSLVGSSTSLSTSMITKYVFGEAGAKIFSLVI
                     GVSLLGWFGVQVGFFAQNAQIIIKDIFNLDVSMQILSLIGGLLMMSTAIYGYKAMEKL
                     SVYSVPFLLVLMMLTIFLAFRANGISVDDNMKSTMTFAGGVSLSMSIIIVGAIVSPDI
                     SRWAKSRRDCALSSFLGIQFGNAFMIIVSIVLVKCMGTSDIMRIFITLGIAIPGIIVL
                     TLAQWTTNTSNVYSASLSIALVLKKAPEKVLTIVLGIIATLLAVFGIYEGFIGFLNLL
                     GIVIAPVGGVYTAEYYIVKQELKGFDKGVLYKPIVKRSIVSWIIGILITYLSTYGFIT
                     LTTIAPLDGFIAGFVVQSIIGKVLCSTKNKQEIDKAV"
     gene            1826..2905
                     /locus_tag="PC414_003432"
     CDS             1826..2905
                     /locus_tag="PC414_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 domain-containing protein"
                     /protein_id="HDF2465801.1"
                     /translation="MRFLTEESVDKIAVGAAVLGTGGGGDPYVGKLVAKQAIKKYGPV
                     KVISLDELDDDALVVPVSGMGSPVITIEKLLSEVELTTPLEIMEKLLNRKVDVIIPIE
                     IGGINSLMPIAVAAKKGLPILDADSMGRAFPEAQMVTFYLEGYEASPAVMSDEKGNSA
                     ILYPVDGIWSERLARTLTVEMGGSSSISDYNLSGAQVKKAAIGNTLTIAETIGGFLLE
                     NKSDSKGAVENILKELCGYKLFEGKVIDIKRELKGGFTRGHAFFAGINEYDGEYSILF
                     QNENLIAKKGDTPLCITPDLIAVLDLETGFPITTERIKYGSRVMVVAFPCNEKWRTEK
                     GIETVGPGYFGYDVEYKTVEELQGK"
     gene            2926..4470
                     /locus_tag="PC414_003433"
     CDS             2926..4470
                     /locus_tag="PC414_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020246462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HDF2465802.1"
                     /translation="MYRVGIDVGGTNTDACILDGELKVIHSVKVATTKDVETGVYNAL
                     KKVIDESKIDHSLIKYAMLGTTHCTNAIVERKRLNNVAMIRIGKPATTAILPFIDWPE
                     DLREKIELDSILVSGGYEFDGRKINELCKDEVVEFCNRIKGKVESVAISGVFAPVNKQ
                     QEEEVAKWVREILGDIPISLSNEIGNIGILERENGAILNSALSQVGKAVSLGFEKALK
                     DLGIDAQIYFSQNDGTLMNLEYTMKYPIFTIGCGITNSIRGASFLSKVKNAIVLDVGG
                     TTSDLGVLYNGFPRESSIPVTIGGVHTNFRMPDVLTIGLAGGTCVKGEKDNIKIGPES
                     VGYRITEDAIIFGGDTLTLSDVVTKLDMAFEDRKSNVENLDKEYCNEVYKKVILKLED
                     AVDQMKTSQDDVELVLTGGGSIIVPEKLKGISNVIRPPHYTAANAIGAALGQISGDVE
                     KVYSLDKMSREDAIEDAKKEAVNKALKAGAKEDTIEVLNIEDVPLAYLPGNALLIKVK
                     VVGDLI"
     gene            complement(4633..5175)
                     /locus_tag="PC414_003434"
     CDS             complement(4633..5175)
                     /locus_tag="PC414_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HDF2465803.1"
                     /translation="MKSYIALLRGINISGKNKIIMSELKASFVELGYSEVSTYLNSGN
                     VVFLSDVDDSKVISNTIRLMIKKQFELEIPVFIISQEELKDILNNAPNWWGDNNKEIY
                     DNLIFMFPDLSYDEFYDEVGNPKEEYEKVYHYKNAIFWSFSRKDYKKTNWWSKTASSN
                     VSDKITIRTANTVRKIVGMR"
     gene            complement(5323..5607)
                     /locus_tag="PC414_003435"
     CDS             complement(5323..5607)
                     /locus_tag="PC414_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF2465804.1"
                     /translation="MAKNTKQTTVHIDETLLKEVKRIGINENKSISQIINESIIDYIL
                     VYRENEDKEKNEHDKNLNETINEFKKEEEKRMQAKSAARAIAEAKKRVLY"
     gene            complement(5670..6434)
                     /locus_tag="PC414_003436"
     CDS             complement(5670..6434)
                     /locus_tag="PC414_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HDF2465805.1"
                     /translation="MNNYLKFATYQLIGFTSSIPRLIKIKKNPDKFSLKEKFEFMQKQ
                     AKKSLDIVNIELNIIGKETLPKEPLLFVVNHSSMLDSFILTASVERPIGCVIADEPVW
                     RNIPIFKEWAKLLRCVYVNRKNNREGIKSIAQASQNILTGQSMAVFPEGDLTWIKEPN
                     SLVSEFRSGALKIAYKAKCPIVPLVIKNSKDTYEGYQPIGKINSVPVEVEFLEPIYDH
                     IENPRLKSSVLGENIKNKMINTIENFRKSNKTFKEF"
     gene            complement(6716..6982)
                     /locus_tag="PC414_003437"
     CDS             complement(6716..6982)
                     /locus_tag="PC414_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HDF2465806.1"
                     /translation="MIESRCGILCYECGYKEKVNCKGCTEIEKPFWGEQCPVKSCCED
                     KNLTHCGLCDTFPCEMLNQFAYDKEQGDDGKRISQCKKWAVLAL"
     gene            complement(7024..7833)
                     /locus_tag="PC414_003438"
     CDS             complement(7024..7833)
                     /locus_tag="PC414_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2465807.1"
                     /translation="MNKIINNILNTCKQYTYILPHKLLRQYIAHYTISIPDTSIKENL
                     TLIPDASGCMIFKFDKKGIESAFWGATTKTTIVKNDIENVLFRVFVEFRPGGVYYLTG
                     LSQRESTDLKISLEDFNTLFSLEVNSIFERTSTIKELVEQLDMLFLSYLLKSNIVDMT
                     IPILENARKQNSIMSVKNISQISCYSERHLNRIFNNSLGMSVKSYLRLLRINLVLQEI
                     QNNKIPFATLAQDIGYYDQSHFINDFKSICGVNPTTYIKNLSDFYNEKYKF"
BASE COUNT         2752 a         1000 c         1284 g         2854 t
ORIGIN      
        1 gaaaatttac atataaaact aaaaaatgca agtaattgac tttttaagtt gacaaaatat
       61 tgcatatata ttatgatata aatcataaat taactacaaa tcaatattat atattcgtag
      121 aagaagaaag tagcataaag gaattttaca gagagttaga tttggctgag aactaatatt
      181 ggaatttatg tgaaaaggac tttggagaat actattgtaa ttatgttaga atagtggttt
      241 aattcgcctt aagaaaatga gtgagtaaaa ttaattttta ctaataagag tggtaacacg
      301 gatttatatt cgtctcttgg atttttccaa gaggcgtttt ttattataaa cttatttcag
      361 aaaatttaaa actatatatt aaatgaaaat tataaaaata tgatagttaa taaatttata
      421 aattagaaaa ttgtaaaata tgctaaggtc agtagtttta cgatttaaat ggagggatga
      481 aaatggaaga agaaaaaatt aactatgctc tggaacagat tccagagaat caaaaacgtg
      541 gttgggtagc tatgttttca gttttagtag caataggtgt ggatttatca tctgttattt
      601 taggcgctga gttggcacag agtatgccaa tgaaacaagc aatattgtca gtaattgttg
      661 gttcattttt ttctgcaatt ttgtatacta catgctcttt ggttggttca tctacaagtt
      721 tatctacatc tatgataaca aagtatgtat ttggagaagc tggagcaaaa atattttctc
      781 tagttatagg ggtttctcta ttaggttggt ttggagttca agtaggtttc tttgctcaaa
      841 atgcacagat aataataaaa gatatattta atttagatgt cagtatgcag attttaagcc
      901 taataggtgg tctcttgatg atgagtactg caatttatgg atataaggca atggagaagt
      961 tgagtgtgta ttcagtacca tttttattag ttcttatgat gctaactata ttcttagctt
     1021 ttagagcaaa tggaatttca gttgatgata atatgaaatc tactatgaca tttgcaggtg
     1081 gagtttctct gtcaatgagt ataataatag ttggtgcaat tgtatctcct gatataagtc
     1141 gttgggctaa aagtagaaga gactgtgctc tttcaagctt tttgggaata caatttggaa
     1201 atgcttttat gataatagtt tcgatagtat tagtaaaatg tatgggaact tcagatatta
     1261 tgagaatttt tattacatta ggaattgcta taccaggtat aatagtactt acattagcac
     1321 aatggactac aaatacaagt aatgtatatt cagcatcatt aagtatagct cttgtactta
     1381 agaaggctcc tgagaaagta ttaactatag tccttggtat tatagcaaca ttattggctg
     1441 tatttggaat atatgaaggt tttataggat ttttaaattt acttggaatt gtaattgcac
     1501 ctgttggtgg tgtatataca gcagaatatt acatagtaaa gcaagaactt aaaggctttg
     1561 ataaaggagt attatataaa ccaatagtta aacgttcaat agtgtcttgg ataattggta
     1621 tattaataac ttacctttca acatatggat ttataactct tactactata gcacctttag
     1681 atggttttat agcagggttt gtagtacaat ctattattgg aaaagtgctt tgttcaacaa
     1741 aaaataagca agaaatagac aaagcagtat aaagaaattt gtttataaag tataaaataa
     1801 attagaaata tgaggagaaa gtaacatgag atttttaaca gaagaaagtg tagataaaat
     1861 agcagttggt gcagcagtgc ttggaactgg aggtggaggt gacccatatg taggtaaatt
     1921 ggttgcaaaa caagccatta aaaagtatgg tcctgtaaaa gtaataagtt tagatgaact
     1981 tgatgatgat gctttggtag ttccagtatc tggtatgggt tctcctgtaa ttacaataga
     2041 aaaattactt tcagaagtag agttaactac ccctttagaa ataatggaga aattattaaa
     2101 tagaaaagtg gatgttataa ttcctataga aataggaggg ataaactcac ttatgcctat
     2161 agcagttgct gctaaaaaag gacttcctat cttagatgca gattctatgg gtagagcttt
     2221 ccctgaagct caaatggtaa ctttttatct tgaaggatat gaggcatctc ctgccgtaat
     2281 gtctgatgaa aaaggtaatt ctgccatcct ttatcctgtg gatggaattt ggtcagaaag
     2341 attagctaga actttaactg tagaaatggg aggaagttct tctattagtg actataattt
     2401 aagtggagct caagttaaaa aagctgctat aggtaatact cttacaatag ctgaaactat
     2461 aggagggttt ttacttgaaa ataaatctga ttcaaaaggt gctgttgaga atatactaaa
     2521 agaattgtgt ggatacaagt tatttgaagg aaaagtaatt gatattaaga gagagttgaa
     2581 aggtggattt actagaggac atgccttttt tgctgggatt aatgagtatg atggagagta
     2641 ttctatatta ttccaaaatg agaatttaat tgccaaaaaa ggtgatacac cactttgtat
     2701 tacaccagat ttaatagcag tattagattt agaaactgga tttccaataa ctacagagag
     2761 aataaaatat ggctctcgtg ttatggtagt agcttttcct tgtaatgaaa aatggagaac
     2821 agaaaaagga attgagacag ttggacctgg atactttgga tatgatgtgg aatataaaac
     2881 agtagaagaa ttacaaggaa aataaaagtt taggaggaaa ttttgatgta tcgtgtagga
     2941 attgacgttg gaggaacaaa tacagatgct tgtatactgg atggggaatt aaaagtaatt
     3001 cattcagtta aggtggcaac tacaaaagat gttgaaactg gtgtttacaa tgccttaaaa
     3061 aaagttatag atgaaagtaa gattgaccat tcattaataa aatatgctat gcttggtaca
     3121 actcattgta caaatgctat tgtagaaaga aaaagactta ataatgtggc tatgataaga
     3181 ataggaaagc cggccactac tgccattctt ccatttatag attggccaga agacttaaga
     3241 gaaaaaatag aattagattc aatattggta tctggtggat atgagtttga tggcagaaaa
     3301 ataaatgaac tttgtaaaga tgaagttgta gagttttgca atagaataaa aggcaaagtg
     3361 gaaagtgttg caataagtgg agtatttgct ccagtaaata aacaacaaga agaagaagtt
     3421 gcaaaatggg ttagagaaat tttaggggat attcctatat ctttatcaaa tgaaataggt
     3481 aatataggta tattagaaag agaaaatgga gctattttaa attctgcatt gtctcaagta
     3541 ggtaaagctg tatctttagg atttgaaaaa gcacttaaag atttaggtat agatgctcaa
     3601 atatatttca gtcaaaatga tggaacatta atgaatttag agtatacaat gaaataccca
     3661 atttttacaa ttggatgtgg aataacaaat tctataagag gagcttcctt cttatcaaaa
     3721 gtaaaaaatg caatagtatt agatgttggt ggaactacaa gcgacttggg tgtactatat
     3781 aatggattcc ctagagaatc atctattcca gttacaattg gaggagtaca tacaaacttt
     3841 agaatgccag atgttttaac tattggtctt gctggtggta catgtgtaaa aggcgaaaaa
     3901 gataatataa agataggtcc tgaaagtgtg ggatatagaa taactgaaga tgcaataata
     3961 tttggtggag acactctaac tttatcagat gttgttacaa aattagatat ggcgtttgaa
     4021 gatagaaaaa gcaatgttga aaacttagat aaagagtatt gtaatgaggt atataagaag
     4081 gttattttaa aattagaaga tgctgtagac caaatgaaga ctagtcaaga tgatgttgaa
     4141 ttggttttaa ctggtggagg aagtataatt gttcctgaaa aactaaaagg cataagcaat
     4201 gttataagac ctcctcatta tactgctgca aatgctattg gtgcagcttt aggacaaata
     4261 agtggagatg tagaaaaagt atacagttta gataaaatgt cgagagaaga tgccatagaa
     4321 gatgcaaaaa aagaggctgt aaataaagct ctaaaagctg gtgctaaaga agatactata
     4381 gaagtattaa atatagaaga tgttccactt gcataccttc ctggtaatgc acttcttata
     4441 aaagtaaaag ttgttggtga tttaatttaa agaataaata taagtatttc cagaattata
     4501 taaatatttc tggactcatt tttaaactta ttttatcaat aaaaacagca taacttttga
     4561 ctatattctt ataagtttcc gaacagccga tatataccta ttttttgagg tttatcggct
     4621 gtttgttatc ttttatcgca ttccaactat ttttcttacc gtatttgctg tacgaattgt
     4681 aatcttatca cttacatttg aactggcagt ctttgaccac caatttgtct ttttataatc
     4741 tttacgacta aaagaccaaa agatagcatt tttataatga tatacttttt catactcttc
     4801 tttaggattt ccaacctcat cataaaactc gtcataagat aagtcaggaa acataaaaat
     4861 aagattatca tatatttctt tgttgttatc tccccaccaa ttaggagcat tatttagtat
     4921 atcttttaac tcttcttgtg aaataataaa aactggtatt tccaattcaa actgtttttt
     4981 tatcattaat cttattgtat ttgagataac cttgctgtca tctacatcac ttaaaaagac
     5041 aacattacca ctattaaggt atgtagaaac ttctgaataa ccaagttcta caaagcttgc
     5101 ttttaattca gacataatta tcttgttctt accacttata ttgatacctc ttaataatgc
     5161 gatataactt ttcatataat aatttcacct ctaaacattc acatttttct atgtagtaca
     5221 aacttgaatt tgcatctttt ataattcttt caaaacttct tcatatgtaa tttttttaat
     5281 ataaaaaaat cggtgcttaa tttgtaccga tttttgtttt tcctagtaaa gaactcgttt
     5341 ttttgcttct gctatagctc tagcagcact ttttgcttgc attctttttt cttcttcttt
     5401 tttaaattca tttattgttt catttagatt cttatcatgt tcattttttt ctttatcttc
     5461 gttttctctg tatactaata tataatctat tattgattcg tttattatct gagatatact
     5521 tttattttca ttaattccta ttctcttaac ttccttaagt agagtctcat caatatgaac
     5581 cgttgtttgc tttgtatttt ttgccacctt aatcacctct taaaaaaaat tctaacatta
     5641 aaattttttt tagtcaataa attttaatat taaaattctt taaatgtttt atttgatttt
     5701 ctaaaatttt ctattgtatt tatcatctta ttttttatat tttcacctag tacagaagac
     5761 tttagtctag gattttcgat atggtcataa attggttcta aaaattcaac ttcgacagga
     5821 acagagttaa ttttaccaat aggttggtat ccttcataag tatcttttga attttttatt
     5881 acgagtggaa caatagggca ttttgcttta tatgctatct ttaaagctcc acttctaaac
     5941 tccgaaacca atgaatttgg ttctttaatc catgttaaat ctccttctgg aaacactgcc
     6001 atactttgac ctgtaagtat attttgagag gcttgggcta tactctttat accctcacga
     6061 ttgttttttc tgtttacata tacacatctt agtaattttg cccattcttt aaatataggt
     6121 atatttctcc aaactggttc atctgcaatt acacatccaa ttggtctttc tacacttgct
     6181 gttagtatga agctgtcaag catactagaa tgatttacaa caaatagaag tggttctttt
     6241 ggcagtgttt ccttaccaat tatattaagc tctatattca ctatatctaa agatttttta
     6301 gcttgttttt gcataaattc aaatttttcc tttaagctaa atttatcagg attcttttta
     6361 attttaataa gcctaggtat agaagatgta aaacctataa gttgatatgt agcaaatttt
     6421 aagtaattat tcataaaatt atctccttaa aataaaatta tttattactg taaagatttt
     6481 aacataaaat aaaatttaag tctattttaa ttcaattttg taacatatat tttaaataga
     6541 cagagattga gattttatat ttccaatttt agtaatcaga ttttatcaat aaattactat
     6601 tctattaatt taattaaata acctttaaat ttaattataa aaactgttgc agaaatttct
     6661 acaacagttt aaaaaattta ccttacaaaa acttatttta taacaagaga gtatattata
     6721 aggctagtac agcccatttt ttgcactgag aaattctttt gccatcatca ccttgttctt
     6781 tgtcatatgc aaattgatta agcatttcac atggaaatgt atcacataga ccacaatgtg
     6841 taaggttttt atcttcacaa caagatttta caggacattg ttcaccccaa aatggtttct
     6901 caatctctgt acaaccttta caattgactt tttccttgta tccacattca taacataaaa
     6961 ttccacatct tgattctatc attttaagtc actccttaaa tataaatttt atatgattgt
     7021 atatcaaaat ttatactttt cattgtaaaa atcggacaaa ttttttatat aggttgttgg
     7081 attaacacca caaatagatt taaaatcatt tataaaatga gattggtcgt aataaccaat
     7141 atcttgtgct aaagttgcaa atggtatttt attattttgt atttcttgta atacaaggtt
     7201 tatacgaagt aatctaaggt atgatttaac actcatacca agagaattat taaaaattct
     7261 atttaaatgt cgttcactat aacaactaat ttgtgaaatg tttttaacag acataataga
     7321 gttttgtttt ctagcatttt caagaattgg tatagtcata tctactatat ttgatttcaa
     7381 taagtatgat aagaataaca tatctaattg ttctactaat tcctttatag tacttgttct
     7441 ttcaaaaatg gaattaacct ctaagctaaa taatgtgtta aaatcttcaa gtgaaatttt
     7501 taaatctgta gattctcttt gagaaagacc tgtcaaataa tatactccac ctgggcgaaa
     7561 ctcaacgaat actctaaaaa gtacattttc tatatcattt tttacaatag tagtttttgt
     7621 tgtggctccc cagaatgcgc tttctatacc ttttttgtca aatttaaata tcatacatcc
     7681 actagcatca ggtattaatg taagattttc tttaatactt gtatctggaa tagatattgt
     7741 atagtgtgct atatactgtc ttaacaattt atgtggcaaa atatatgtat actgtttgca
     7801 ggtatttaaa atgttattga ttattttatt cataagtata tcctcctgat atataaaaaa
     7861 taagtatact aatttatttt ttatataata
//
LOCUS       DAMJVK010000139         7831 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.144, whole genome shotgun sequence.
ACCESSION   DAMJVK010000139 DAMJVK010000000
VERSION     DAMJVK010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7831
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.144"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            438..1739
                     /locus_tag="PC414_003439"
     CDS             438..1739
                     /locus_tag="PC414_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2465808.1"
                     /translation="MGKSKDVEIEKSILGTILLDNKLSYKLDELNENMFMNDICLEIF
                     KIMKELKKENIVIDVATVKSKIDRKSLAIKTSDVTNLITWGQNFGLDGHIKILKENLA
                     RRSINQNCQNLLHSLNLGENIDTCIYKFESNIKEILDKDTYENDDVNSIAGKVLDFLE
                     NKKDIGFKFGIKLLDTTIGGLFKGELTTIAAKSGVGKTALALQIMLNSFKQGKKTLFI
                     SREMTSEQVFMRNICRVTGVSTRNMKSKEIDENDWKLIVNAIGDLSENNLIYINDKID
                     TISAIRKRIRQVKPDLLIVDYVQLLTSQKSMDKREREVATFSRELKNMTLDFNIPVIQ
                     LSQLNDEMKDSRPYGDRPMRDSKAIYHDSNNVVYIHQLKGSDYEEAVRDIGESEEAVR
                     ASEYRGIKMVDLIVAKCRDGQTRHKHFCYFGDKLHFQELNY"
     gene            1815..2090
                     /locus_tag="PC414_003440"
     CDS             1815..2090
                     /locus_tag="PC414_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF2465809.1"
                     /translation="MLELRKIIELLEEHKIEVSIPKELHKYSLLEYLELCQQSGGTYF
                     YIKTLEGALKEARNELMIKLFNGKNYMELARMFEMSVINVRHIVRKK"
     gene            3425..3655
                     /locus_tag="PC414_003441"
     CDS             3425..3655
                     /locus_tag="PC414_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2465810.1"
                     /translation="MSNKKKKYANILANECVACGSCIKACPRSAISVPCGISAKINRD
                     LCVGCGICEKICPASVIEIITILKEEEGKCHE"
     gene            3648..4229
                     /locus_tag="PC414_003442"
     CDS             3648..4229
                     /locus_tag="PC414_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2465811.1"
                     /translation="MNNKKHWYDYLWIFSSIYLILGFVNIIFAWIGLICFFVPLAISI
                     VKGNKAYCNKYCGRGQLFNLLGNKLKLSRNRDIPKFIRSKWFRYGFLTFFMVMFANML
                     FSTYLVFEGSRNFKEVVTLLWTIKMPWQWAYHGTLVSPWVVQFAFGFYSVMLTSTVLG
                     LITMILFKPRSWCVYCPMGTMTQMICKAKSKSV"
     gene            complement(4808..5485)
                     /locus_tag="PC414_003443"
     CDS             complement(4808..5485)
                     /locus_tag="PC414_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDF2465812.1"
                     /translation="MISQNEIDILTHSLPFWDKLTDLQKELLISSANTSHYKKGNPVH
                     CGDSDCIGILIIKSGTVRTYILSDEGREVTLFRLDDGDVCILSASCILKTITFDVYVD
                     AETDCDIIQISSSVFAKLSTENIHAELFSYKLATERFSDVMWAMQQILFMSFDKRLAS
                     FLIDEIAKNGSSTINMTHEQIAKYMGSAREVVSRMLKYFAREGIVSLSRGGIKVLDKD
                     KLRSLTL"
     gene            complement(5674..7038)
                     /locus_tag="PC414_003444"
     CDS             complement(5674..7038)
                     /locus_tag="PC414_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2465813.1"
                     /translation="MKESNKRIELLGSAPISKALLSMGLPTMIGMMITALYNLVDAYF
                     VGGLGTSQMGAISVAFPLGQLVVGLGLLFGNGAASYVARLLGHGDNEKAGKVVSTSIY
                     SSIIVGTLVIIVIMIFLNPILKLLGATKSIMPYAISYASIYVVSSIFNIFNVTMTNIV
                     TSEGAAKTTMCAMLLGALLNMILDPIFIYTFGLGVRGASIATAISQIASTLVYVGYIL
                     SKKSIFSFRINECCFSKEIFSEILKIGIPTLIFQLLTSLAITLTNMEATKYGDSVIAG
                     MGAVTRITSLGSLVVFGFIKGFQPIAGYNFGSKNYDRLHIAIKTSILWTTVFCVVLGL
                     ILILFPKSIISQFTSGDILLIENGSKALRANGFSFMLFGFYTVYSTLFLALGKAKEGG
                     FLGMCRQGVCFIPIILIIPVFYGLNGILYAQPIADLMAAIIAGFMSIRLHKELNLLKI
                     NGMH"
     gene            7166..7687
                     /locus_tag="PC414_003445"
     CDS             7166..7687
                     /locus_tag="PC414_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDF2465814.1"
                     /translation="MATIDLIVLGIVKQQPKSAYDIQKAVEYRNISRWVKISTPSIYK
                     KVIQLEQKGYIESKTVKNGKMPEKAVYSLTELGETHFLDLMFDTSSKPINILLDFNAV
                     IVNLNSLTPEKQKLCLNNIEENVQELKKTIEENISIKTEVPDTAKAVLEQQLVLAQAL
                     ETWINSLKDNMGN"
BASE COUNT         3066 a          886 c         1343 g         2536 t
ORIGIN      
        1 atatcaataa ttggtagtaa attatattaa gtaagatatt actatcaggt agtgaattat
       61 attgaacgat atattaataa ttggcagtaa attatattaa ataagatatt aatatcgagt
      121 aatgaattat attgaacgat atatcaataa ttggcagtaa gttatatcaa ataagatatt
      181 actatcgagt aatgaattat attgaacaat atattaataa ttggcagtaa gttatatcaa
      241 ataagatatt gctattatgt agtaaattat ataaaacaat gtatcagaat aaatagtata
      301 ttaataattt gtagtatatc atgacgaata atacagtcat aacaaatagt gagtcataac
      361 aaataatgta tttataattg ataatgtatt taaaaatcaa aatataaaaa cttataagga
      421 aagagagatt aatttatatg ggtaaatcaa aagatgttga aatagagaag tctattttag
      481 gcacaattct tttagacaat aaattaagct ataagctaga tgaattaaat gaaaacatgt
      541 ttatgaatga tatatgtctt gagatattta aaattatgaa agaacttaaa aaagaaaaca
      601 tagtgattga tgtggccact gtgaaatcaa agatagatag aaaatctcta gcaataaaaa
      661 caagtgatgt gactaatcta attacttggg gtcagaattt tggtcttgat ggtcatatta
      721 aaattttaaa agaaaattta gctagaagaa gcataaatca aaactgccaa aaccttttac
      781 acagcctaaa ccttggagaa aatatcgata cttgtattta caaatttgaa agtaatataa
      841 aggagatttt ggacaaggat acttatgaaa atgatgatgt aaatagtata gcagggaagg
      901 tgcttgattt tttagaaaac aaaaaagata ttggatttaa atttggtata aaattacttg
      961 ataccactat aggaggtctt tttaaaggag agctaaccac aatagcagct aaatcggggg
     1021 ttgggaagac tgctttggca cttcaaataa tgttaaattc atttaagcaa ggtaaaaaaa
     1081 ctttatttat aagtagagaa atgaccagtg agcaagtatt tatgagaaat atatgcagag
     1141 taactggtgt aagtacaagg aatatgaaat ctaaagaaat agatgaaaat gattggaaac
     1201 ttattgtaaa tgcgataggt gatttaagcg aaaataactt aatttatatc aatgataaga
     1261 tagatacaat tagtgcaatt agaaagagga taaggcaggt aaagccagat ttattaatag
     1321 tggattatgt gcaactcctt acatctcaga aaagcatgga taagagagaa agggaggtag
     1381 ctactttttc gagagaactt aaaaatatga ctttagattt taatatacct gtaatccaat
     1441 taagtcagtt aaatgatgaa atgaaagatt caagacctta tggagataga cctatgaggg
     1501 atagcaaagc catctaccac gatagcaaca atgtggttta tatacatcaa ttaaagggga
     1561 gtgattatga agaagcagtg agagatattg gagaaagtga ggaggcagtt agagcatcag
     1621 aatacagggg aattaagatg gttgatttaa tagttgcgaa gtgtagagat ggacagacta
     1681 ggcataagca tttttgttac tttggggata agttacattt tcaagaacta aattattagg
     1741 tcaataaagt agtaataaca agcttattaa aagtcgggaa atagcaaata gattataaaa
     1801 tatgaggtga aaatatgttg gaattaagaa aaattataga attacttgaa gaacacaaga
     1861 tagaagtaag tattccaaag gagttacata agtatagttt attggaatac cttgaattgt
     1921 gtcaacaaag tgggggaaca tatttttata ttaaaacact ggaaggggct ttaaaagagg
     1981 ctagaaatga gcttatgatt aaattgttta atgggaaaaa ttatatggaa ttagcgagga
     2041 tgtttgaaat gagtgttatt aatgtcaggc atattgttag aaaaaaatag atatttttgg
     2101 agaatacaag tgtgatatac ttctgaaata gaagttatta taagaggagt ataagtgtga
     2161 ttaacaacat gattttgtga ataaaattga ggatatatat gagttaaatg tgtgattaat
     2221 aaggtaagat tgtgtataag attatgagta tttaaaagga ggggctataa ttaatagtgt
     2281 agagttgtgt gtttgcttaa gattatataa taatttaata gattaaacta tactaataag
     2341 gtaaaatcgt gtgtttagtt aaagcttatt ataaaatctt gatatgtgat taatgatgca
     2401 atatgtgaat aaagttaaga ttataataga tttaacctgt ggctaataga ataaaattgt
     2461 gaataaagtt aagattataa taggtttaaa ttgtgactaa tagagtaaga gtgtaaatag
     2521 agataaactt caatatgagc ttaacctatg attaatagag taagaatgta aatagagata
     2581 aacttcaata tgagtttaac ttgtggctaa tagagtaaga gtgtaaatag agataaactt
     2641 caatatgggt ttaacttgtg gttaatagag taaggtgtaa ctaaagataa acttcaatat
     2701 gagcttaacc tatgattaat agagtaagaa tgtaaataga gataaacttc aatatgagtt
     2761 taacttgtgg ctaatagagt aagagtgtaa ctaaagataa acttcaatat gggtttaact
     2821 tgtggttaat agagtaagaa tgtaaataga gataaacttc aatatgagct taacttgtga
     2881 ttaatagagt aagagtgtaa atagagataa acttcaatat gggattaact tgtgattaat
     2941 agagtaagag tgtaactaaa gataaacttc aatatgagct taacctatga ttaatagagt
     3001 aagaatgtaa atagagataa acttcaatat gggtttaacc tgtggttaat agagtagggt
     3061 gtaactaaag ataaacttca atatgagttt aacttgtggc taatagaata agattgtgaa
     3121 taaagttata gttttagatg ggtattaacc tgttactaat agaatatgat taggtgtaaa
     3181 attatagagc tgcataagaa aatattatag atatattaaa tattagataa taaatattta
     3241 tatagataca caaaagaaaa ggcgatatga agctaaactt aatgacttca tatcgttttt
     3301 tatttgaaac tcctttgtat ttagagtgtg atttttaata ttgaggtttt atgtgattaa
     3361 gtcacagata aaaagtaagt atcttgatat aattcttata gattaataat atagggaggt
     3421 tattatgtct aataaaaaaa agaagtatgc aaatatttta gcaaatgagt gcgttgcttg
     3481 tggtagctgt ataaaagcat gtccaagaag tgctatctca gttccatgtg gcattagtgc
     3541 aaaaattaat agagatttat gtgttggatg tgggatttgt gaaaagatat gtccagcatc
     3601 tgtaattgag attataacaa tattaaaaga ggaagaaggt aagtgtcatg aataataaaa
     3661 aacattggta tgactattta tggatttttt cttcaatata tttgatattg ggatttgtaa
     3721 atattatttt cgcttggatt gggctaattt gtttctttgt tccactagct atttctattg
     3781 ttaagggaaa taaagcttac tgtaataagt attgtggaag agggcaactg tttaatttgc
     3841 ttggaaataa attgaagctg tcaagaaata gagatattcc taaatttatt agaagtaagt
     3901 ggtttagata tgggtttttg acatttttta tggtaatgtt tgccaatatg cttttttcaa
     3961 cttatttagt gtttgaagga agtagaaatt ttaaagaagt agtgactttg ttgtggacta
     4021 taaaaatgcc ttggcaatgg gcgtatcatg gaactttagt ttcaccttgg gtggtacagt
     4081 ttgcatttgg tttttatagt gttatgctta catcaactgt tcttggtttg attactatga
     4141 tattgtttaa acctcgttca tggtgtgttt attgccctat gggtactatg actcagatga
     4201 tatgtaaggc aaagagtaag tctgtataga aaggccttat gtgatattta cagttttgtt
     4261 caagtagaga tgagtagtag gtttgaatat atatgacaaa tatatgagta taggggcata
     4321 gatttagttt gtgtaaggta aactgattta tgcttctttt ttatgtctta gattattggc
     4381 taagtggaaa caatgaattt agatattaag aattattcag ttatttgaag aaaatagaat
     4441 aataattaac attatattaa tggatattca ttacatataa gccagttaat agcaagattt
     4501 atttaatatg atataggtca agtatttaaa ctaaataagg tcatgacaat taatataagt
     4561 atagatataa aaagattaga agttataact tataaaaggt attctatata aatagtatgg
     4621 ggaaaatagt caaattaact tattagaaac aaaaaatata cataaaaaat aaatgtaaat
     4681 atcaaataag atactatatc aaaatagata taagtatctt ttttattatt gaaattatga
     4741 aaatatattt gattttacac aaaaaagagt gcctcatgaa ctaaaaagtt cattttgaga
     4801 cacactttta cagtgtcaaa gaacgaagtt tatccttatc caataccttt ataccaccac
     4861 gagataaaga cacaatacct tctctagcaa aatatttcaa cattctcgat acaacttctc
     4921 tggcactacc catatactta gcaatttgct catgagtcat atttatagtg ctactgccat
     4981 ttttagctat ctcatcaatt aaaaaagatg ccaaacgctt atcaaaactc ataaaaagaa
     5041 tttgttgcat agcccacata acatcagaaa aacgttcagt agctaattta tatgaaaata
     5101 actctgcatg aatattttct gtagataatt ttgcaaaaac agaggaacta atctgtataa
     5161 tatcgcagtc cgtttcagca tctacataga catcaaaagt tattgttttt agaatacagg
     5221 aagctgataa aatacataca tctccatcat caagtcggaa cagtgtaacc tctcgacctt
     5281 catctgaaag tatgtatgta cgcactgttc cagattttat aatcaaaata ccaatacagt
     5341 cactatcacc acaatgtact ggattccctt ttttgtaatg cgaggtattt gctgaagaaa
     5401 ttaataattc tttttgtaag tcagtaagtt tatcccaaaa aggcaagcta tgtgtaagta
     5461 tatcaatttc attttgtgaa atcatacaat atctcctttc ttaaattgtt ataatcccat
     5521 gatacaagtg aatagataaa ttgacaagta gtttgttaag aggattattt agtcagatag
     5581 taatagtatg atttatgtag aaaaaatatg ataatatata ttcttgctat taatgagaaa
     5641 gtaatatgtt ttgatgatag taggtttgta taactaatgc ataccgttta tttttaaaag
     5701 attaagttcc ttatgtaacc ttatagacat aaaaccagca attatagcag ccatcaaatc
     5761 agctattggt tgtgcatata agataccatt taacccatag aatacaggaa taatcaaaat
     5821 tatagggata aaacagacac cttgtctaca catacctaaa aatccacctt cctttgcttt
     5881 tcctagtgcg aggaataatg tagaatacac tgtatagaat ccaaatagca taaatgaaaa
     5941 cccatttgcc ctaagagctt tactaccatt ttctattaat aaaatgtcac cacttgtaaa
     6001 ttgtgaaatt atagattttg ggaagagtat caatataaga ccaagcacta cacaaaatac
     6061 tgtagtccat aatatggaag tttttattgc tatatgtaat ctatcgtagt tctttgaacc
     6121 aaaattataa ccagcaattg gttggaaacc ttttataaaa ccaaatacaa caagactacc
     6181 taaagacgtt attcgtgtta cagcacccat tccagctatc acagaatcac catactttgt
     6241 agcctccata ttagttaatg taatagcaag actagtcaat aattgaaata ttaaggtagg
     6301 tatgccaatt tttaggattt ctgaaaaaat ttcttttgaa aaacagcatt cgttaattct
     6361 aaaactaaat atacttttct tgcttaaaat atatcctaca taaacaagtg ttgaagcaat
     6421 ctgtgagata gctgttgcaa tagaagcacc ccttaccccc agaccaaaag tataaatgaa
     6481 tatcgggtct aaaatcatat taagcaaagc acctaataac atagcacaca tagttgtttt
     6541 agcagctcct tcacttgtga caatattagt catagtgaca ttgaatatat taaaaataga
     6601 tgacacgaca taaatgctag cataagatat tgcataaggc ataatacttt ttgtagctcc
     6661 taacaatttt aaaattggat tgagaaaaat cataatcaca ataataacta atgtacccac
     6721 tataatacta ctgtaaatag atgtacttac cacttttcct gctttttcat tatcaccatg
     6781 gcctaaaagt ctggcaacat atgaagcagc tccatttcca aacaataatc ctaatcccac
     6841 aacaagctga cctaatggaa atgcaacgga tatagctccc atttgacttg ttccaagtcc
     6901 acccacaaag tatgcatcaa ccaaattgta taatgcagtt atcatcatac ctatcatggt
     6961 aggtaatccc atagataaca atgcttttga gattggggca gaccctaata attcaattct
     7021 tttgttactt tctttcatac ttaacactcc tcttctattg acattaagtc taaattataa
     7081 tactataaat tatagtaagt atggaaaata gtactataat ttatagtatt tgtcaatagt
     7141 aagaaaacta aaggagatga tttttatggc tacaatagat ttaattgtat tgggaatagt
     7201 aaagcaacaa cccaagagtg cttatgatat tcaaaaggca gtggaataca ggaatatatc
     7261 acgttgggtt aaaataagta caccatcaat ttataaaaaa gtaattcaat tagaacaaaa
     7321 gggatatatt gaaagtaaaa ctgtgaagaa tggaaaaatg ccagaaaaag cagtttattc
     7381 attaactgaa ttaggtgaga cacatttttt agatttaatg tttgatacat ctagcaaacc
     7441 aataaatatt cttttagatt ttaatgctgt tatagtaaat ttaaatagcc ttacaccaga
     7501 aaaacagaaa ttatgtctca ataatattga agagaatgtt caagagttaa agaaaactat
     7561 tgaggaaaat ataagtataa agacagaagt accagacaca gcaaaagcag tgttggagca
     7621 acagttggtg ctagcacaag cattggaaac ctggataaat tctttaaaag ataatatggg
     7681 aaattagaat tagagaagct aacttaataa tcattaaaca aaaaccagca tatttaataa
     7741 tgagatatta gaaaaaataa aacaaatgat ataaactgaa aagtatattt aaaataaagc
     7801 tcacttaatt gagggagaat gttaaacttt t
//
LOCUS       DAMJVK010000140         7808 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.145, whole genome shotgun sequence.
ACCESSION   DAMJVK010000140 DAMJVK010000000
VERSION     DAMJVK010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7808
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.145"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(<1..1332)
                     /locus_tag="PC414_003446"
     CDS             complement(<1..1332)
                     /locus_tag="PC414_003446"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HDF2465815.1"
                     /translation="MINIEIPKKVDYIIKELEKNGYEAYIVGGCVRDCLLERVPNDWD
                     ITTSARPEVVVELFEKTIPTGIQHGTVTVMIEHEPFEVTTYRIDGNYSDGRHPDSIEF
                     TNNIVKDLSRRDFTINSIAYNSKTGLVDPFNGYEDIQNKYIRCVGNPVDRFEEDALRM
                     LRAVRFSAQLNFKIAEGTKQSIHKKADLIKNVSIERIQTEFNKILVSDSSKLNLLKST
                     GLLKFIIPEICELEDVTQHNPYHIYDVQKHTLIATEVIEDELYLKLTMLFHDLGKKVT
                     KTTDKNGVDHFYTHSRESVKIAKKILKRLKYDNYTINKVLILIQYHDYRIEPKRKIIK
                     KLLNKLEDVELFEDLIKVNWADTLAKNPKYAKQKILNLIECEKEFKHIINQKECFNLK
                     DLAINGKDLISIGIKPGKDIGYILNKMLEIVINNPELNEKEILKEKALNIYT"
     gene            complement(1481..3286)
                     /gene="lepA"
                     /locus_tag="PC414_003447"
     CDS             complement(1481..3286)
                     /gene="lepA"
                     /locus_tag="PC414_003447"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDF2465816.1"
                     /translation="MDNKQSRTRNFSIIAHIDHGKSTLADRLIQQTGLVSERDMKSQL
                     LDNMDLERERGITIKLQNIRLMYKAKDGNEYYLNLIDTPGHVDFNYEVSRSLAACEGA
                     LLVVDAAQGVEAQTLANVYLAIDQDLEILPIINKIDLPSARPEEVKNEIEDLIGLDSS
                     EAPLISAKTGLNIEDVLEDIVKNVPPPKGDNEAPLKALIFDSYYDAYKGVVAYVRVFE
                     GTVKKGMTIKMMNTNKKFEVTEVGVMAPGQTELSELSAGDVGYIAASIKDIRSCRVGD
                     TITDSNNPTEEPLPGYKKATPMVYCGIYPGEGEKYENVRDALEKLQVNDAALEYEAET
                     SAALGFGFRCGFLGLLHMEIMQERLEREFNLDIITTAPSVIYRVTKMDGEVVMIQNPA
                     NLPEPSEIKMIEEPIVKGDIIVPKDYVGVVMELCQERRGNMLNMEYIDDRRVMLHYDL
                     PLNEVVYDFFDALKSRTRGYGSLDYEVKGYVASTLVKLDILINKEQVDALSFIVHETR
                     AFPRGKAMCEKLKGEIPRHQFAIPIQAAVGNKVIARETISALRKDVLAKCYGGDISRK
                     KKLLEKQKEGKKRMRQIGSVEVPQKAFMSVLKLDE"
     gene            complement(3568..3819)
                     /locus_tag="PC414_003448"
     CDS             complement(3568..3819)
                     /locus_tag="PC414_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465817.1"
                     /translation="MSRLEKNIESKSKLRKYKYIMRILFLITMIICTSVCVFIVDSNA
                     KSMLGMKQYDFPSIKFDITKFDFDIPNFDIHEIIKKFNK"
     gene            complement(3850..4869)
                     /locus_tag="PC414_003449"
     CDS             complement(3850..4869)
                     /locus_tag="PC414_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HDF2465818.1"
                     /translation="MFKKCIKVVTLTFILVCILPGKSLALNQDDFLKFLVNSSYPEAK
                     VEGNDTENKKNNKNKETSKENKEESKEGNTKSKDASKVDNKKESEKEYIKLYVGKENV
                     PDIESKNSDTTETNTTSSSDYKDDLRVTKENPRILIYHTHGCETYSNSPDGNYHSRDK
                     KNSVMEVGSALTSALDSKGWGVVHTTKYHDYPSYNNSYASSLKTIQSILPKYNSVDIA
                     IDLHRDARDLTNPATKEKDHLKYTTMINGERVSKFFFVVGGKNTNRKQLRALAEDITA
                     FAEKKYPGLVSPIVEKDYARFNQFAVKNHMLVEIGNNATSVEESKATTKYLAEILDEY
                     FKQKN"
     gene            complement(4887..5840)
                     /locus_tag="PC414_003450"
     CDS             complement(4887..5840)
                     /locus_tag="PC414_003450"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HDF2465819.1"
                     /translation="MISVRTDLALEASEMCEKSQEGSSIPGVKIETKELENCIVTKVE
                     VIDRQGAEIMNKDIGKYITLESNLMKFDDDESREEMISYLKEELVDIFGQDKNKKTLV
                     IGLGNRNITSDALGPKSVSKTLVTRHLFKNYNKDYDDDFTEVSALSPGVMGVTGIETS
                     EIVKSLVEKVKPDRVVAIDALASRKMERVNSTIQISTAGISPGGGVGNTRKSLTKETL
                     GVDVIAIGVPTVVDAATLTIDVLDMAIDNLIAQSEETESFYEMLKKLKEEEKYHLIKD
                     SLDPYDKNLIVTPKDIDDTIENLSIIISEGLNRSLHPGRLV"
     gene            complement(6182..6958)
                     /locus_tag="PC414_003451"
     CDS             complement(6182..6958)
                     /locus_tag="PC414_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2465820.1"
                     /translation="MKFHEFGNKNRPHIMLIHGGGNAWWNYLRQAEVLSNKYHVILPT
                     LDGHGEEYETTYISTEDTADKLISYIDENCSGHLFALCGVSLGGQIVMELLSRKPNIT
                     KKAIIDGSICYPRPLMARFCIAGIRFFGGLLFSEKACRFQIAMMPKLLPENMQYPDKI
                     KAYYMTDMPHVRKETFYNMYRTYMMNYTLKENVKKTTAQVMYWYGEKEMKCVKKSAKL
                     FQSYVPSCEIYEAKSYNHGYLSLYLPNEWLEIAEPFFQKE"
     gene            complement(6992..7513)
                     /locus_tag="PC414_003452"
     CDS             complement(6992..7513)
                     /locus_tag="PC414_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2465821.1"
                     /translation="MDIAKLILNPARLRILQYIRLYGSVRTSDIVKYLNDIPRATVYH
                     HVKILEENNMIEVIKENRVRGTIEKVYALKEYTTSMEGETFVALSTAFYVGLMQEMNE
                     YFSRKEQDHKKDNVFFSSALLYMSDNEYENLLKSIADLLKPYIEQKPKSNLKLRKLSI
                     ISSPPVADNDKTK"
BASE COUNT         2414 a         1388 c          873 g         3133 t
ORIGIN      
        1 tgtatatata tttaatgctt tttcttttag tatttctttt tcatttaact ctggattatt
       61 tatgactatt tcaagcatct tatttaaaat atatcctata tctttacctg gttttatacc
      121 tatagatatc aaatcttttc cattaatagc taaatcttta agattaaagc actccttttg
      181 atttattata tgtttaaatt ctttttcaca ctcaataaga tttaatatct tttgttttgc
      241 atactttgga ttttttgcaa gtgtatcagc ccaattaact tttattaaat cttcaaataa
      301 ttcaacatct tcaagtttat ttaataactt tttaattatc ttccttttag gttctattct
      361 ataatcatga tattgtatta atattaaaac tttattaata gtataattat catacttaag
      421 tcttttcaaa atcttctttg caatctttac agattctcta ctatgagtat aaaaatggtc
      481 aacaccattt ttatcagttg ttttagttac ttttttacct aaatcatgaa acaacattgt
      541 aagttttaaa tacagttcat cttcaattac ttctgtagct attaaagtat gtttttgtac
      601 atcatagatg tgatatggat tgtgttgagt aacatcttct aattcacata tttcaggaat
      661 tataaatttc aacaaacctg ttgattttaa taagtttaat ttagatgaat cagatactaa
      721 tattttatta aactctgtct ggattctttc aatagaaaca ttttttatca aatctgcttt
      781 tttatgaata ctttgcttag taccttcagc tattttaaaa ttgagttgtg ctgaaaatct
      841 aactgctcta agcattctaa gtgcatcttc ttcaaatcta tctactggat ttccaacaca
      901 acgtatatac ttattttgta tatcttcata gccattaaat gggtcgacta atcctgtctt
      961 tgaattgtat gcaattgaat tgatagtaaa atctcttctt gacaagtcct taactatgtt
     1021 atttgtaaat tctattgaat ctggatgtct accatcacta taatttccat caattctata
     1081 agtcgtcact tcaaatggtt catgttcaat cataactgtt acagtcccat gttgtatacc
     1141 agttggaatt gttttttcaa ataattctac taccacttct ggtctagcac tagttgttat
     1201 atcccaatcg tttggaactc tttctaataa acaatctcta acacatcctc caactatata
     1261 agcttcataa ccattctttt ctaattcttt aattatatag tctacttttt taggaatttc
     1321 tatattaatc ataaatatca cctttttatt cttctttaat attcttactt atattctcta
     1381 tgagtttact taaaatatac cataaaaaag ctagactaat ttagaaataa ttctaaaatc
     1441 actctagctt ttaaatatct ttatctaaat ttataaggat ttactcatct aatttaagca
     1501 cagacataaa tgctttttga ggaacttcaa cagaaccaat ttgtctcata cgcttcttac
     1561 cttctttttg tttttcaaga agtttcttct tacgagatat atctccacca taacatttag
     1621 caagaacatc ttttcttaga gcgcttattg tttctcttgc aataacctta ttaccaacag
     1681 cagcttgtat tggtatagca aactgatgtc ttggaatttc tccttttaat ttctcacaca
     1741 ttgcctttcc tctagggaaa gcacgtgtct catggacaat aaaactaagt gcatccactt
     1801 gctctttatt gattaatata tcaagtttta caagtgtaga tgctacataa cctttaacct
     1861 cataatcaag agaaccataa cctctagttc ttgattttaa agcatcaaag aaatcgtaaa
     1921 caacttcatt gagaggaagg tcatagtgaa gcattactct tctatcatct atatattcca
     1981 tatttaacat atttccacgt ctttcctggc aaagctccat aacaacaccc acatagtctt
     2041 taggaactat tatatctcct tttactatag gctcttctat catctttatc tcagatggtt
     2101 ctggtaagtt tgcaggattt tgtatcatta caacctctcc atccattttt gtaactctat
     2161 atataacaga tggagctgta gtaattatat caaggttaaa ttctctctca agtctttctt
     2221 gcattatctc catatgtaaa agacctaaga aaccacatct aaaaccaaat cctagtgctg
     2281 ctgaagtctc agcttcatat tcaagtgcag catcatttac ttgaagcttt tcaagagcat
     2341 ctctaacatt ctcatacttt tctccttctc ctggatatat accacaatat accattggag
     2401 tagctttttt gtatcctggt aaaggttctt ctgtaggatt atttgaatct gttattgtat
     2461 caccaacacg acaacttctt atatctttta tactagcagc aatataacca acatcacctg
     2521 ctgataattc actaagttca gtttgacctg gagccataac accaacttca gttacctcaa
     2581 actttttatt ggtattcatc atttttattg tcataccctt tttaacagtt ccttcaaata
     2641 ctctaacata agctacaaca cctttgtaag catcatagta agaatcgaat attaatgcct
     2701 ttaatggagc ttcattatct cctttaggtg gaggaacatt ttttacaata tcttctaaaa
     2761 catcttctat gtttaaacca gttttagctg aaataagagg agcttcactt gaatcaagcc
     2821 ctattaaatc ttctatttca ttttttactt cctctggtct tgcacttggt aaatctattt
     2881 tatttattat tggtaatatt tctaaatctt ggtctatagc aagataaaca ttggctaaag
     2941 tttgtgcctc aacaccttga gctgcatcaa ctactaatag tgctccttca catgcagcta
     3001 aacttcttga tacctcatag ttaaagtcta catgtccagg tgtatctata agatttaaat
     3061 aatattcatt tccatccttc gctttataca ttaatcttat gttttgaagc tttatagtga
     3121 ttcctctttc tctttcaagg tccatattgt caagtaattg acttttcatg tctctttcac
     3181 taacaagccc agtttgttgt attaatctat cagctaaggt agactttcca tggtctatat
     3241 gtgcaattat actaaaattt ctagttctac tttgtttatt gtccacctta aaattccctc
     3301 ctcttgatta ttcttatagt tattatttta cattatctaa aaactcttta tataccaaat
     3361 ttaaaatttt tgaatataaa ctaattggca ctaaacaaaa ttatccacat tatatgtgga
     3421 ttatcaagtc taacactaaa tattatataa tatcataaac ttaaagtaaa tcctataaaa
     3481 tacagttaat gaatttttat tttatacatt aaaaatatat aactaaaata aggcttattt
     3541 taatcaataa accttattca cttattacta cttattaaat ttttttatga tttcatgtat
     3601 atcaaaattt ggtatgtcaa aatcaaattt tgttatatca aatttaatac ttggaaaatc
     3661 atattgtttc attcctagca tactttttgc attactgtcc actataaata cacaaactga
     3721 tgtacatata atcatagtaa tcaaaaataa gattctcata atatacttgt attttcttag
     3781 tttactttta ctttctatat ttttttctaa tcggctcatt tagcgtgctc ctaattataa
     3841 cttaaagctc taattttttt gtttaaaata ttcatccaaa atctcggcta aatactttgt
     3901 agtagcctta gattcttcta cacttgttgc gttattccct atttctacta acatatgatt
     3961 cttaactgca aactgattga atctagcata gtctttttct actattggag atactagacc
     4021 aggatacttt ttctcagcaa aagcagttat atcttctgct aaagctctaa gttgttttct
     4081 atttgtattt ttacctccta caacaaagaa gaactttgat actctctctc catttatcat
     4141 agttgtatat tttaaatggt ctttttcctt agtggctgga ttagttaaat ccctagcatc
     4201 tctatgtaag tctatcgcta tatccactga gttatattta ggtaatatac tttgtattgt
     4261 ttttaagcta cttgcataag agttattata agatggatag tcatggtatt tagtagtatg
     4321 gactacaccc catcctttgc tatctaaagc actagttaat gcacttccta cttccattac
     4381 tgaatttttc ttatctcttg aatgataatt tccatcaggt gaatttgaat aagtctcaca
     4441 tccatgagta tgatatatca aaattcttgg attttctttg gttactctta aatcatcctt
     4501 ataatctgaa ctagatgttg tgttagtttc agttgtgtct gaattttttg actcaatatc
     4561 tggaacattt tctttaccaa cataaagttt tatatattct ttttcactct cttttttatt
     4621 atcaacttta cttgcatctt tagatttagt gtttccttct ttactttctt ccttattttc
     4681 cttacttgtt tctttatttt tattattctt tttattttct gtatcatttc cttcgacttt
     4741 agcttctgga taagatgaat ttactaaaaa ctttaaaaaa tcatcttgat tcaaagctaa
     4801 tgattttcct ggtaaaatgc atactaatat aaaagtcaag gttactactt taatgcattt
     4861 tttaaacata attgctcctc cttattttat accaatctac ctggatgtaa agacctattt
     4921 aagccttcac ttattattat agatagattt tcgattgtat catctatatc ctttggtgtt
     4981 actattaagt tcttgtcata tgggtctaac gaatctttta ttagatgata tttttcttct
     5041 tctttaagtt ttttcaacat ttcataaaaa ctttctgttt cttctgattg agctattaaa
     5101 ttgtctattg ccatatctaa gacatctatt gtaagagttg ctgcatcaac caccgttgga
     5161 acgcctattg caataacatc cacgcctaaa gtttctttag ttagagattt tctagtattg
     5221 ccaacacctc caccaggtga tattcctgct gttgatatct gtatagtaga gtttactctt
     5281 tccatttttc ttgaagccaa tgcatctatt gctactactc tatctggctt aaccttctca
     5341 accaatgatt ttactatttc actagtttct attccagtaa cacccataac tcctgggctt
     5401 aaagctgata cctcagtgaa atcatcatca tagtctttat tataattttt aaaaagatgt
     5461 ctagttacaa gtgtttttga tacactcttt ggtcctaacg catcagaagt aatatttcta
     5521 tttccaagac caattaccag ggttttttta tttttgtctt ggccaaatat atctactaac
     5581 tcctctttta aataacttat catttcttct cttgactcat catcatcaaa cttcataaga
     5641 ttactttcca gagttatata ctttcctata tctttattca ttatctctgc gccttgcctg
     5701 tctattactt caactttagt tacaatgcaa ttttctaatt ctttagtttc tatttttaca
     5761 ccaggaatac tacttccttc ttgagacttt tcacacattt cactagcttc taaagctaaa
     5821 tctgttctta cactaatcat ataaacctcc agtataaatt aaatgctaat tataattgtt
     5881 aacaaaattt agtattttat aacaagaaat tgtaaattta tttaaaacaa gtaaaattaa
     5941 ggctttcaat gtaagaaaat tgggtttagt cagtgatttg tcagtaaacc ttcttatcta
     6001 tcatttattt acaaacagaa aaaggtagca actatttata taggtgctac ctttaatcta
     6061 tttataattt tcctattaag ctttcactaa tatattaact aaaataattt tttatattta
     6121 tacctaattt atttactata taaaaaaaca gcaataatta tacaataatc acctaacacc
     6181 tttattcctt ttggaaaaat ggttctgcaa tttccaacca ttcatttgga agataaagtg
     6241 ataaataccc atgattatag ctcttagctt cataaatctc acaagagggc acataagact
     6301 gaaacagttt tgcagatttt ttgacacact tcatttcttt ttctccatac cagtacatta
     6361 cttgtgctgt ggttttttta acattctctt taagtgtata gttcatcatg taagtacgat
     6421 acatattata gaatgtttct tttcttacat gtggcatatc tgtcatataa tacgctttta
     6481 ttttatctgg atattgcata ttttcaggta acaattttgg catcatagca atttgaaagc
     6541 gacaagcctt ctcactaaac aaaagtcctc caaaaaatcg aataccagca atacaaaacc
     6601 ttgccataag aggtcttggg tagcaaatac taccatcaat aattgctttt ttagtaatat
     6661 taggttttcg agataaaagt tccatgacaa tttgaccacc aagtgatacc ccacacaaag
     6721 cgaaaagatg tccactgcaa ttttcatcta tatatgatat caacttatct gctgtatcct
     6781 ctgtagaaat gtatgttgtt tcatattctt ctccatgacc atccaatgta ggaagtatca
     6841 catgatattt gttagacaaa acttctgctt gtcgcaaata gttccaccat gcattccctc
     6901 ccccatgaat caacataata tgtggtctgt ttttatttcc aaactcatga aatttcatat
     6961 ataacctcct tttataaaaa tgggttgcat gttattttgt tttatcatta tcagctacag
     7021 gtggagaaga tataattgaa agttttctaa gttttagatt tgatttaggt ttttgttcta
     7081 tatatggttt caataaatca gctatacttt taagaagatt ttcatattca ttatcactca
     7141 tatatagtaa tgctgaagag aaaaatacat tatccttttt atggtcttgc tcttttctgc
     7201 taaagtattc attcatttcc tgcatcagac ctacataaaa tgctgtagaa agtgcaacga
     7261 aggtctctcc ttccatagat gttgtatatt cctttagtgc ataaaccttt tcaatagtac
     7321 cacgcacacg attttcttta attacttcaa tcatattgtt ctcttctaaa atttttacat
     7381 ggtgataaac tgttgcacga ggaatatcat ttaaatactt tacaatatca gatgtacgaa
     7441 cactgccata aagtcgaata tattgtaaaa tacgaagcct tgcaggattt aaaattagtt
     7501 ttgctatgtc catcttatca gccctttctt tagtctataa aaatattata gtctattttt
     7561 ttagactatt caagtctatg atataaatag taattacaac tataataaat gctacaaatg
     7621 ctattctata taaacactga cattaattac atataacctt ctaacatatt agttatattg
     7681 tttgatgcat ctatcataca tactaatgaa aaaaattatc ttaattaagc atataaattt
     7741 atatttttat aatttttcat taaatttaca aaaatgagta atttcttgta ttatcttata
     7801 attattgg
//
LOCUS       DAMJVK010000141         7772 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.146, whole genome shotgun sequence.
ACCESSION   DAMJVK010000141 DAMJVK010000000
VERSION     DAMJVK010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7772
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.146"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            135..1193
                     /locus_tag="PC414_003453"
     CDS             135..1193
                     /locus_tag="PC414_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HDF2465822.1"
                     /translation="MNCKWISKIDERKKCHREADSSGYCIFHKENKSDEEIQLMMDTI
                     HKEEISEFNGFVFENEFNAEEILTYNYKILDFSESIFKQKANFKKYIFKKNIIFNYTE
                     FRDKVLFNGCVFLENCDFNRSIFSKNYINDRIFEKVKFKGPDLVVNKVENFPRMDGII
                     FSMCTKFVLKNVEYGKSEYEHGKINYRIARNQATKIGEYEMIGFYYYKERIYSSKIMK
                     RSNYPTFSDYLVEKFFDQIARYTTGYGEKPWNILLVIIAIISVFALLYLFVGIESSNS
                     TLVALDINNIGDYSLSEIFKMYMDLWYFSMATFSTVGYGDMVATSLIGKALAGIEVFF
                     GVTIGAIWASVIIKRMIR"
     gene            1297..2481
                     /locus_tag="PC414_003454"
     CDS             1297..2481
                     /locus_tag="PC414_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDF2465823.1"
                     /translation="MKNALKKIISLAMILVFIIPNYSYADDKSVDKYSKTSILIDQET
                     GRVLYSKEPDMKVPLASLSKMMTFLLAIEAIENKEVKETDKVKIDKSIASVKGSSYKL
                     KDGEEVPLIELMRGLMIVSGNDAAIAIAKHICKTKEAFVDRMNKKAKEIGMSNTHFLN
                     PNGLPIYDLSNPKKPAKENISTAKDIAILGKYMFDHYEKQVTAITDMETYTNSDRSFE
                     KSNTNALLRIIPEVDGIKTGYTGNAGYCLSFSMIVNKNDKNEKKRRVIGVVLGTNHKN
                     KRTSASLALLKYGKENFNMKKVIDKDSFIGKKYIKGMKELEVTLKAKDELYTILKDDE
                     SIKSEVKIKNIEYPVKKGDTMGIIKYYTNSGDLLGSVDIVSNNSIDNVSLKTKLKMKF
                     AN"
     gene            2688..3401
                     /gene="rpsB"
                     /locus_tag="PC414_003455"
     CDS             2688..3401
                     /gene="rpsB"
                     /locus_tag="PC414_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HDF2465824.1"
                     /translation="MSVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIYIIDLQ
                     KTVKKVEEAYKFTKEVAETGKPILFVGTKKQAQDAIKDEAERCGMYFVNERWLGGMLT
                     NHKTIKTRINKLRELEKMEEEGVFNVLPKKEVIKLRAEKEKLEKYLGGIKDMPELPGA
                     MFVVDPRKENIAIQEAHRLGIPVVGIVDTNCDPEQLDFAIPGNDDAIRAVKLITGAMA
                     TAVIEGRQGAEEEVAEDQE"
     gene            3480..4391
                     /locus_tag="PC414_003456"
     CDS             3480..4391
                     /locus_tag="PC414_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HDF2465825.1"
                     /translation="MANITAQMVKELRESTGAGMMDCKKALQEAEGNMEKAVDLLREK
                     GLSKAAKKAGRVAAEGLVAIEMNDDNTVASMVEVNSETDFVAKNEDFKVFVKDAACMA
                     LATDKEDIASLLGETHKEGITLQEVLNNRVAKIGEKLDFRRFAKVVTNGQVAGYIHGG
                     GKIGVLVEMETEARDAKVLELGKDVAMQVAAMNPKYVSRDEVDAEYIAHETEVLTQQA
                     LNEGKPANIVEKMVKGRLEKELKEVCLLEQTFVKNPDITVKQLVADVAKAVGSDIKVV
                     KVVRFEVGEGIQKREENFAEEVAKQLK"
     gene            4476..5180
                     /locus_tag="PC414_003457"
     CDS             4476..5180
                     /locus_tag="PC414_003457"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HDF2465826.1"
                     /translation="MDKPMYKRVLLKLSGEALAGERGFGINNDVVNDIAIAIKKIQEI
                     GVEVAVVVGGGNFWRGRTSEGMDRTTADYIGMLATVMNAMALQDALENIDVATRVQTA
                     IDMRQIAEPYIRRRAVRHLEKERVVIFGAGTGNPYFTTDTTAALRAAEMEAEVILLAK
                     NVDAVYDKDPKVHADAKKFTELSYMEVIQKELKVMDSTATSLCMDNKIPIKVFELTTE
                     NIIRAVKGENIGTTVK"
     gene            5206..5763
                     /gene="frr"
                     /locus_tag="PC414_003458"
     CDS             5206..5763
                     /gene="frr"
                     /locus_tag="PC414_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HDF2465827.1"
                     /translation="MKLEIHKQLEEKMNGTIDALKFEFGTIRAGRANAQMLDKIRVDY
                     YGTPTPINQIGAISVPEPRILMISPWDKSAMHEIEKAIANSDLGLNPSNDGEVIRLSV
                     PALTEERRKELAKKASKAAEEFKVRIRNERRDANEKIKKMEKGGELTEDELKKAQDEV
                     QKMTDKFIKEIDALLSKKEKDIMEV"
     gene            5766..5969
                     /locus_tag="PC414_003459"
     CDS             5766..5969
                     /locus_tag="PC414_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465828.1"
                     /translation="MKKYYNLLGLHVDEVKQYFDEQNINYTVKSIEGKKDKEKLVVPR
                     VIKISEIGDSVELIITYFSDSLV"
     gene            5998..6732
                     /locus_tag="PC414_003460"
     CDS             5998..6732
                     /locus_tag="PC414_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HDF2465829.1"
                     /translation="MNNNIIYDIDLNNIPTHIAIIMDGNGRWAKARFLPRTAGHKAGV
                     ETIRDIVKECSKLGVKHLTLYAFSTENWKRPKLEVDTLMNLLSTYLRNEIAELHQNNV
                     KVTAIGDISALPKTCIRELNSAKELTKNNTGVNLNLALNYGSRADIKNALIDIVKNCE
                     SGKIDINNIDEDIIKNYLSTKSIPDPDLVIRTSGEQRLSNFLLWEVAYSEFYFTDIHW
                     PDFKKEELQKAIYVYQRRDRRFGGLK"
     gene            6745..7524
                     /locus_tag="PC414_003461"
     CDS             6745..7524
                     /locus_tag="PC414_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDF2465830.1"
                     /translation="MLTRIIASLALVPLFLFVVYGGIPLYIAETAIVYIALHEFYKAF
                     KIKDVHPIFIIGYLFSIYLAVKNIFNLPLEYTYAVIFILFLASIIYMLMGKNNVIDVS
                     ITFLGVFYIGVFLDFIIITINGFEKGSIYVWLIFVISFMTDIFAYFSGYLLGKHKLIP
                     KVSPKKTIEGAIGGIIGSTLCCILFGYLFGIDLLQLAIIGSIGSVIAQLGDLFASSIK
                     RYVGIKDYGKIIPGHGGILDRFDSVILVAPFVYSAIKFFIR"
BASE COUNT         3211 a          789 c         1464 g         2308 t
ORIGIN      
        1 acaatgaagt agattaaaaa tttaaaacta agaatctaat atatattttg taactattta
       61 agtatataaa ataaaataat atccaaacta atacaaaata gtaataattg ttttgtactg
      121 gaggatatat atttatgaat tgtaaatgga tatctaaaat agatgagaga aaaaaatgtc
      181 atagagaagc agattcttct ggatattgta tctttcataa agaaaataag tctgatgaag
      241 aaatacagtt gatgatggat actattcata aagaagagat aagtgaattt aatggatttg
      301 tttttgaaaa tgaatttaat gcggaagaaa tattaactta caattataaa atactggatt
      361 tttctgaatc catatttaaa caaaaagcaa attttaaaaa atatattttt aaaaagaata
      421 ttatttttaa ctatacagaa tttagagata aagttttatt taatggttgt gtattcttag
      481 aaaactgtga ttttaataga agcatattta gtaaaaacta tataaatgac cgtatatttg
      541 aaaaagtgaa atttaaagga cctgatttgg tagttaataa agtagaaaat tttccaagaa
      601 tggatggaat aatttttagc atgtgtacaa aatttgtgtt aaagaatgtt gaatatggaa
      661 aaagtgagta tgagcatggc aagataaatt atagaatagc tagaaatcaa gcaacaaaaa
      721 ttggagaata tgagatgata ggattttact attataagga aagaatttat agtagtaaaa
      781 taatgaaacg ttctaattat cctactttca gtgattattt agttgaaaaa ttttttgacc
      841 aaatagcacg ttacacaaca ggttatggag aaaagccatg gaatatatta ttagttatta
      901 tagcaataat aagtgttttt gctttattat acttatttgt aggcatagaa agttcaaata
      961 gcacacttgt agctttagat ataaataata taggtgatta ctcactaagt gagattttca
     1021 aaatgtatat ggatttatgg tattttagca tggctacatt tagtacagta ggatatggag
     1081 atatggttgc tacaagtttg attgggaaag cattggcagg tatagaagta ttttttggag
     1141 ttacaatagg agcaatttgg gcatctgtga ttattaaaag aatgataagg taatatttgt
     1201 gtaaatatgg ataggtatta attactagat ataaattgga ctaaataggg aaaatttaaa
     1261 tataaatata cttaataatc caagaaggtg aaaaatttga aaaatgcact aaaaaaaatt
     1321 atatcattag ccatgattct agtttttata ataccaaatt attcatatgc agatgataaa
     1381 tctgtagaca agtattcaaa aacatctata cttatagacc aagaaacagg tagagtttta
     1441 tatagtaaag aacctgatat gaaggtacca ttagctagtt taagtaagat gatgactttt
     1501 ttacttgcaa tagaagctat tgaaaataaa gaagtaaaag aaactgataa agtaaaaata
     1561 gacaaatcta ttgcatcagt aaaagggtca agttataaac ttaaagatgg tgaagaagtg
     1621 ccacttatag agcttatgag aggtcttatg attgtatcag gaaatgatgc ggcaattgct
     1681 atagcaaaac atatttgtaa aacgaaagaa gcatttgtag atagaatgaa taaaaaagca
     1741 aaagaaatag gaatgagtaa tacacatttt ctaaatccaa atggtcttcc aatatacgat
     1801 ttaagcaacc ccaaaaaacc agcaaaagaa aatatctcta ctgcaaaaga tatagctatt
     1861 cttggaaaat acatgtttga ccattatgaa aaacaagtaa cagctataac tgatatggag
     1921 acatatacaa attcagatag aagttttgaa aaaagcaata caaatgcatt attaagaatt
     1981 ataccagaag tagatggtat aaaaactgga tatactggca atgctggata ttgcttatca
     2041 ttttctatga ttgtaaataa aaatgataaa aatgagaaaa aacgtagagt aataggggtt
     2101 gtattaggta ctaaccataa aaataaaaga acctctgctt cattagctct actgaaatat
     2161 ggaaaagaaa atttcaatat gaaaaaagta attgataaag atagctttat tggtaagaaa
     2221 tatataaaag gaatgaaaga gttagaggtt acattaaaag caaaagatga actttataca
     2281 atattaaaag atgatgaaag tataaaatca gaagttaaga ttaaaaatat agagtaccct
     2341 gttaaaaaag gtgatactat gggtattata aaatattata ctaattcagg tgacttactt
     2401 ggaagtgtag atatagtaag taataatagt atagataacg tatcattaaa aacaaaatta
     2461 aaaatgaaat ttgcaaatta aattcaaaaa atggtataaa atacacaaaa aaatataaaa
     2521 atagaaaata tggaaaaaat attgttattt cttcacctct gtgatatact actagaggtg
     2581 aaaaaataaa ataaatcaca cacagcattg ggatccttaa aaggtgccta ttacatggtt
     2641 ttttagggag aagatcaagc tggaggtata aaaccaggag gtaagatatg tcagtaatat
     2701 caatgaaaca attattagaa gcaggtgttc actttggtca ccaaacaaga agatggaacc
     2761 ctaaaatggc taagtatata ttcacagaga gaaatggaat atatataatc gacttacaaa
     2821 aaacagttaa aaaagttgaa gaagcttaca agttcactaa agaagtagct gaaacaggaa
     2881 agccaatctt attcgtagga actaaaaaac aagctcaaga tgcgataaaa gatgaagctg
     2941 aaagatgtgg aatgtacttt gttaatgaaa gatggttagg tggaatgtta acaaaccaca
     3001 aaactataaa aacaagaata aataaattaa gagaattaga aaaaatggaa gaagaaggcg
     3061 tatttaatgt tcttcctaaa aaagaagtta taaaattaag agctgaaaaa gaaaaactag
     3121 aaaaatactt aggtggaata aaagacatgc ctgaattacc aggtgcaatg ttcgtagttg
     3181 acccaagaaa agaaaacata gctattcaag aagctcatag attaggtata ccagtagttg
     3241 gtatagttga tactaactgt gacccagaac aattagactt tgctatacca ggaaatgatg
     3301 acgctataag agccgtaaaa ttaataactg gtgctatggc aacagctgta attgaaggta
     3361 gacaaggtgc tgaggaagaa gtagctgaag accaagaata ataatttgac aattaaatgg
     3421 taagggtcgc ccttaccatt tataatatgg aatagaataa gtaggaggaa acttaaaaaa
     3481 tggctaatat aactgctcaa atggtaaaag agttaagaga aagtacaggc gcaggaatga
     3541 tggactgcaa gaaagcttta caagaagctg aaggaaacat ggaaaaagca gtagatttat
     3601 taagagaaaa aggattatca aaagctgcta aaaaagctgg tagagttgct gctgaaggtc
     3661 tagttgcaat agaaatgaat gatgataata cagttgcttc tatggtagaa gttaactcag
     3721 agacagattt cgttgctaaa aacgaagact tcaaagtgtt cgttaaagat gctgcttgta
     3781 tggcattagc tactgataaa gaagatatag cttctttatt aggtgaaact cataaagagg
     3841 gaataacttt acaagaagtt ttaaataata gagttgcaaa aataggtgag aaattagact
     3901 ttagaagatt tgctaaagtt gtaactaatg gacaagttgc tggatacatt cacggtggtg
     3961 gaaaaatagg tgttcttgtt gaaatggaaa cagaagctag agatgctaaa gttttagaat
     4021 taggtaaaga tgtagctatg caagttgctg ctatgaatcc taagtatgtt tcaagagatg
     4081 aagtagatgc tgaatacata gcacatgaaa cagaagtatt aactcaacaa gctttaaatg
     4141 aaggaaaacc agctaatata gttgaaaaaa tggttaaagg aagattagaa aaagaattaa
     4201 aagaggtttg tttattagag caaactttcg ttaaaaatcc agatataact gttaaacaat
     4261 tagttgctga tgttgctaag gcagtaggtt ctgatataaa agttgttaaa gttgtgagat
     4321 tcgaagttgg tgaaggtata caaaagagag aagaaaactt tgcagaagaa gttgctaagc
     4381 aacttaagta ataaccaaag agagaacacg caggtgttct cttttttaaa aaatagaaaa
     4441 aattggatat gaaggattga aataggaggt ttctgatgga taaaccaatg tacaaaaggg
     4501 tattgttaaa attaagtgga gaagcattag caggagaaag aggttttggt ataaataatg
     4561 atgtagttaa tgatattgct attgctataa aaaaaataca agaaataggt gttgaagtag
     4621 ctgtagtagt aggtggagga aacttctgga gaggcagaac cagtgaagga atggacagaa
     4681 ctactgctga ctatatagga atgttagcta cagttatgaa tgcaatggcg ctacaggatg
     4741 cactagaaaa tatagatgtt gctacaagag ttcaaactgc tatagacatg agacaaattg
     4801 cagaaccata tataagaaga agagcagtta gacacttaga aaaggaaaga gttgttatat
     4861 ttggtgcagg aacaggaaac ccttacttta caacagatac aactgctgct ttacgtgctg
     4921 ctgagatgga agcagaagtt atattgttag ctaaaaatgt tgatgcagta tatgacaagg
     4981 atccaaaagt acatgcagat gctaagaaat ttacagaatt aagttacatg gaagttatac
     5041 agaaagaatt aaaagtaatg gattctacag ctacatcttt atgtatggat aataaaattc
     5101 caataaaggt atttgagctt acaacagaga atataataag agcagtaaaa ggcgaaaata
     5161 taggaactac tgtaaaataa tgaagaatga aggaggagta aaattatgaa actggaaata
     5221 cataagcaat tagaagagaa aatgaatggg acaattgatg ctttaaagtt tgaatttgga
     5281 actataagag caggaagagc taatgcacaa atgctagaca aaataagagt tgattattat
     5341 ggaactccaa ctccaataaa tcaaataggt gctatatctg taccagaacc aagaatatta
     5401 atgatatctc cttgggataa atctgctatg catgaaatag aaaaagcgat agctaactca
     5461 gacttaggat taaatccatc aaatgatggt gaggttataa gactttctgt acctgcttta
     5521 acagaagaaa gaagaaaaga attagctaaa aaagctagta aagctgcaga agaatttaaa
     5581 gttagaataa gaaatgaaag aagagacgcc aatgagaaaa ttaaaaagat ggaaaaaggc
     5641 ggagaattaa ctgaagatga attaaaaaaa gcacaagatg aagttcaaaa aatgactgat
     5701 aaatttataa aagaaataga tgcattatta tctaaaaaag aaaaggacat aatggaggta
     5761 taatattgaa aaaatattat aaccttttag gtcttcatgt agatgaggta aaacaatatt
     5821 ttgatgagca aaatataaat tataccgtaa aatctattga aggtaaaaag gataaagaaa
     5881 aacttgtagt tcctagagtt ataaaaatat ctgagatagg tgatagtgta gaactgataa
     5941 tcacatactt ttctgactcc ttagtttaag gagtcagaac aatattggag gcaaaatatg
     6001 aataacaaca ttatatatga tatagactta aataacatac ctactcatat tgctattata
     6061 atggatggaa atggaagatg ggcaaaagct agatttcttc caagaactgc aggacataag
     6121 gctggagtag aaactataag agatatagta aaagaatgct caaaattagg tgttaagcac
     6181 ttaacattat atgcattttc aacagaaaac tggaaaagac cgaaacttga agttgacaca
     6241 cttatgaact tattatctac atacttaaga aatgaaatag ctgaacttca ccaaaacaat
     6301 gtaaaagtaa ctgctatagg agatataagt gctttaccaa aaacttgtat aagagaatta
     6361 aactcagcta aggaactgac aaaaaacaat acaggagtta acttaaattt agcattaaac
     6421 tacggaagta gagcagatat aaaaaatgct cttatagata tagtaaaaaa ttgtgaaagt
     6481 ggtaaaatag atataaacaa tattgatgaa gatattataa aaaactattt aagcacaaag
     6541 tctataccag atccagactt ggtaataaga acaagtggtg aacaaagatt gagtaacttt
     6601 ttactttggg aagttgctta ttctgagttt tactttactg atatacattg gcctgatttt
     6661 aaaaaagaag aactacagaa ggctatatac gtatatcaaa gaagggatag aagatttgga
     6721 ggacttaagt aaggagatta ttttatgctt acaagaatta ttgcttcact agctctggtt
     6781 ccattatttc tatttgttgt atatggaggt attcctctat atatagctga gacagcaata
     6841 gtttatattg cattgcatga gttttataag gcatttaaaa taaaagatgt acatccaata
     6901 ttcattatag gatatttatt ttctatttat ttagctgtaa aaaatatttt taatttacca
     6961 ttagaatata cttatgcagt aatttttatt ttatttttag caagtataat ttatatgttg
     7021 atgggcaaaa acaatgttat agatgtttcg attacgtttt taggagtttt ttatatagga
     7081 gtatttttag attttataat tataactata aatggatttg aaaaaggtag tatatatgta
     7141 tggcttatat ttgtgatatc ttttatgaca gatatatttg catattttag tggttattta
     7201 cttggaaaac ataagctaat tcctaaagtt agtcctaaaa agactataga aggagctatt
     7261 ggaggaataa taggaagtac actttgttgt attttatttg gatatttatt tggcattgat
     7321 ttgcttcaat tagcgataat aggaagtata ggaagtgtaa tagctcaatt aggagattta
     7381 tttgcatctt ctataaagag atatgtaggg ataaaggatt atggaaagat aatacctggt
     7441 catgggggta tattagatag atttgatagt gttatattag ttgcaccttt tgtatatagt
     7501 gcgattaaat tttttattag ataaagattg gttggattta aaagagcaat ctataattgc
     7561 tagtaatgaa aatagctatg taacttttaa atgcagagat attctgcagt tattagttac
     7621 atggctattt tcattgttca aaaatttaga agaccttggg gaattgtata agaataggta
     7681 ttactttata atagtatact atatttgtag tgagtatatt ttttatactg taactatcat
     7741 taaaaaatat ttttttgttt aataatttcg at
//
LOCUS       DAMJVK010000142         7770 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.147, whole genome shotgun sequence.
ACCESSION   DAMJVK010000142 DAMJVK010000000
VERSION     DAMJVK010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7770
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.147"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(318..980)
                     /locus_tag="PC414_003462"
                     /pseudo
     CDS             complement(318..980)
                     /locus_tag="PC414_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440163.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
     gene            complement(1140..1676)
                     /locus_tag="PC414_003463"
     CDS             complement(1140..1676)
                     /locus_tag="PC414_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HDF2465831.1"
                     /translation="MIGLKCGMVKLLEHQMIWDKSAKDVIILLKSIWDKTAIDIQHIG
                     STSIPSISAKPIIDIVVGVASLEEAKLYLERLEQCGIVFRGQDVPKQLLFAMGDFEKN
                     TRTHHIHVVEWNSVAWNNYINFRDYLNTFTDKAKLYDSHKQKLALQFEDNRKIYTQRK
                     QELINILLEEARLWRINT"
     gene            complement(1749..2162)
                     /locus_tag="PC414_003464"
     CDS             complement(1749..2162)
                     /locus_tag="PC414_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HDF2465832.1"
                     /translation="MKMPKESIDTIMFAPCGMNCMVCYKHCYHKKPCAGCLNGDTGKP
                     EHCRKCKIKDCVCQKGLFYCFECFNFPCKLIKNLEKSYNKRYQASLMENSKFVQQYGL
                     EKFMQKQKERYTCSKCGGIISIHDRECSECQEKVK"
     gene            2628..3182
                     /locus_tag="PC414_003465"
     CDS             2628..3182
                     /locus_tag="PC414_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HDF2465833.1"
                     /translation="MDEKILELLQEMQGNISNMQGSISGIEGTISDMQSNINGIEDTI
                     SGMQGTISDMQGSISSMNGTISDMQGSISSMDGTINSMQDSITDMQVEIKEVNSRLDN
                     MEPDIKDLKTGQVETSSRLDTIQSDVTEIKSDVKINRNTLDTVIKHTSSLVKDTSDIK
                     NDLNNIEIVTSKNSNDIAKLKSVK"
     gene            complement(3428..3583)
                     /locus_tag="PC414_003466"
     CDS             complement(3428..3583)
                     /locus_tag="PC414_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465834.1"
                     /translation="MVKFNQSLKVSKEKIITLKQEDINGKMKTRFYPFISDKGMCFNQ
                     RWTFILG"
     gene            complement(3790..3969)
                     /locus_tag="PC414_003467"
     CDS             complement(3790..3969)
                     /locus_tag="PC414_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF2465835.1"
                     /translation="MLQTGKQGADKKYLDNMFFVKGYVEELLFLDNSYDIVFSCIVFH
                     HCTNVQNIFCIFWKW"
     gene            complement(4195..4728)
                     /locus_tag="PC414_003468"
     CDS             complement(4195..4728)
                     /locus_tag="PC414_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDF2465836.1"
                     /translation="MQYISYYHSPIGNILLASDNIGLTGLWFENQKYYAYNLDQEHQE
                     KELPIFEQTKKWLDIYFSGKEPNFTPSLHMVGTPFQTTVWKILQQIPYGKTITYGEIA
                     YKVAQQKGISKMSAQAIGGAVGHNGISIIIPCHRVVGTNGSLTGYAGGIDRKIKLLSL
                     EKVSVQHYFIPKKGSAL"
     gene            5045..5395
                     /locus_tag="PC414_003469"
                     /pseudo
     CDS             5045..5395
                     /locus_tag="PC414_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729451.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5364..5683
                     /locus_tag="PC414_003470"
                     /pseudo
     CDS             5364..5683
                     /locus_tag="PC414_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429539.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(6476..7048)
                     /locus_tag="PC414_003471"
     CDS             complement(6476..7048)
                     /locus_tag="PC414_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HDF2465837.1"
                     /translation="MWIYQKTLEHPVNIRQADPRMAKYIMTQLGGPNGELAAATRYLQ
                     QRYTMPTGKSRALLTDIGTEEMAHVEIISSVLYQLIGNCTPEELKAAGLGSNYANFGH
                     GLQPVDSNGVNFTTSYINVFGDSVTDLHEDMAAEQKALATYYQLINLTDDPDLKDILR
                     FLGEREVVHYQRFGEALMDVYEFTECKHQF"
     gene            complement(7110..7373)
                     /locus_tag="PC414_003472"
     CDS             complement(7110..7373)
                     /locus_tag="PC414_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HDF2465838.1"
                     /translation="MSLNRRELLERISEYQFACIELNLYLDNNPRDKKALDSYNRYCD
                     KFTQAVCDYESKYGALTNFGYESSEYPWSWISEPWPWDKSFYK"
     gene            complement(7373..7594)
                     /locus_tag="PC414_003473"
     CDS             complement(7373..7594)
                     /locus_tag="PC414_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HDF2465839.1"
                     /translation="MGYLKEDKQDYCCEFARPYINRQIYDDTYNPKTALKRGTLFPEL
                     DLIESNNYNDWLYANPKNRVRAKKGGNMY"
BASE COUNT         2688 a         1332 c          927 g         2823 t
ORIGIN      
        1 atagcttgta gtgtacttcc ctcaagagga atagtcgttg aagtttctcc tattcggagt
       61 ttacctgctg attgcccatt ttaaagtact tagggtttaa ccttatacca tctaaccaat
      121 tttttctact ttcgtaactt tcacacttag gtttatttca tccttatgtt ttagtttggt
      181 tagctttagg ggtttccagc aattcaagta gtattggata ggttctatcc tatctctaca
      241 tataggtttc cctatatgct gactatttta gctactaact catgactaaa gtcacaagtg
      301 ttcgtagcta ttttttaatc aaactggaat acagactcca ttcctaggaa attggctgaa
      361 atatagtctg ctgtcaaaga tgctgtcatc acagaaatta aaacagatac tgaaaagttt
      421 ttatggactt cttcaagtga aaacataaca cccgctagtg gtgcatgaaa ggctgctgat
      481 aatccagcac ttgccccaca ggttaacagg aatttttcct ctgtcttccc tcggtcaaga
      541 gctctagaaa tacctttacc tgccatagca ccaagctgaa tggaaggacc ttcccttccc
      601 aaagcaagac cacccatgag tgataagaat cctccaataa attttgttgg caacactcgc
      661 caccatactt gttttatttt ccctgtcatt tccccctcaa gctgagggat accacttcca
      721 gaaatcattg gctcccagtc caccaacttt gatacaatcc acgcaagtac tgcaagtact
      781 gcaaaccaag ctattatttt tattgtagaa ccttctacaa attttaaaat ttggctaagc
      841 caagtaccag cctgccctaa aagtatcctg tataaaagca caatcaaacc agccacagcc
      901 ccaaccataa gtccttcacc aatcaaaatt acctgaaagt gctctgcccg tttgataata
      961 tgagatgtat cctttttcat actattcctc cttcatccaa taattctaca caatctgaat
     1021 ctccaatgac taaagtcatg agtgtgtaca gatatttttt aatcaacaaa aatagatttt
     1081 tacttactaa atataagaaa ataattgctc aatttctcaa taaatgtgaa tttattcttt
     1141 taggtattta ttctccacag tcgtgcttct tccagcaaaa tatttattaa ttcttgcttt
     1201 ctctgtgtat agattttacg attgtcctca aactgtaatg ctaatttttg tttatgacta
     1261 tcatagagtt ttgctttgtc tgtaaaagta tttagataat ctcgaaaatt gatataatta
     1321 ttccatgcaa cactattcca ttcaacaaca tgaatatggt gagtacgagt atttttctca
     1381 aaatctccca tagcaaaaag taattgtttt ggtacatcct gacctcgaaa aacgattcca
     1441 cattgctcta acctttcgag gtataattta gcttcctcta aagaagcaac acctacaaca
     1501 atatcaataa taggctttgc gctaatactg ggaatagaag tgcttccaat gtgttgaata
     1561 tcaatagcag tcttatccca tatacttttt aataatataa tgacatcttt tgcactttta
     1621 tcccatatca tctgatgttc caataattta accataccac attttaaacc tatcactata
     1681 taccacctca atataatcca cctttatata tattacaatt ttttctatct tgataaatag
     1741 tgatttagct atttcacttt ttcttgacac tcgctacatt ccctatcatg aatagatata
     1801 attcctccac atttagaaca agtatatctc tccttctgct tttgcataaa cttctctaaa
     1861 ccgtactgct gaacaaattt actattttcc ataaggcttg cttgatatct cttgttataa
     1921 cttttttcaa gattttttat gagtttacaa ggaaaattga aacattcaaa acaataaaac
     1981 aacccttttt gacagacaca atcctttatt ttgcacttac ggcagtgttc tggctttcct
     2041 gtatcgccgt tcaaacaacc agcacatggc tttttgtgat aacaatgctt atagcaaacc
     2101 atacaattca ttccacaagg agcaaacata atcgtatcaa tactctcttt tggcattttc
     2161 ataacatttt actcctttca aaaatccaat ttgtcgaatt gttatatatt catattgtaa
     2221 cataaaaaat agctaaaaaa ttcatgcact ttcattgcca ataaattttc aaggaaatat
     2281 gaaagtttaa aactaagaat aaaaagagat aaaaaggaag ctactaatca aaatattcaa
     2341 acacaagtaa tcaatataaa taacttaagg tatatttaaa agtatatgat attaccagca
     2401 gttttaaata caaatataac cacacatgcc tcaaaaacac ttattacagt atcttagaaa
     2461 cttagacaat acataagatt cattaataaa aaagttctca atatagatat atttaaccat
     2521 gttttgaata gtagataatc atatgattaa aaattaaata tgacatagcg tttgtggaat
     2581 ataacaccta aattatgtta taataataaa gcaggagtgt gatagtgatg gatgaaaaaa
     2641 tattagagtt attacaggaa atgcaaggta atattagtaa tatgcaaggt agtatcagtg
     2701 gtatcgaagg cactattagt gatatgcaaa gcaatattaa tggtatcgaa gacactatta
     2761 gtggcatgca aggcactatt agcgatatgc aaggcagtat cagtagtatg aatggcacta
     2821 ttagcgatat gcaaggcagt atcagtagta tggatggcac tattaatagt atgcaagaca
     2881 gtattactga tatgcaagtt gaaattaaag aagtcaatag taggttagac aatatggaac
     2941 ctgatattaa agacttaaaa actggacaag ttgaaactag tagtagactt gatactattc
     3001 agagtgatgt tacagaaatt aagtcagatg taaaaattaa cagaaataca ttagatacag
     3061 tgattaaaca tacctcttca ttagtaaaag atacatcaga tattaaaaat gatttaaata
     3121 atatagagat agtcacatct aagaattcga atgatattgc taaattaaaa tcagttaaat
     3181 aatatactat agtaaaaaat acctgaaaat ttcacatact tctttaaagt tataccaaat
     3241 tttatacaga aatactgatt atccgacaac acactttact tataaataat ttattaaaaa
     3301 aatactcatt ttatcctaaa attaacacga ctaaatgagt cttgatataa taataaaaaa
     3361 taagggagat tgaacactaa aaagttgtgt atctccctta tattctataa tatagttata
     3421 taatttttca gcctaaaata aaagtccatc tttgattaaa acacatcccc ttatcactta
     3481 taaaaggata aaatcttgtt ttcatttttc catttatatc ttcttgctta agagtaataa
     3541 ttttttcttt tgatacttta agtgattggt taaatttaac caaacgacta agttttgttc
     3601 aacatctatt tttcatattt tccaatactt aatctatttt ttataaataa ttcttttatt
     3661 tctaactcac ttacattttt tacataaaac gaagcatata aatttcaacc ttatcttgta
     3721 tggttcttaa tacttaatga gtagcttaag tatcaatcat tataaatttt tcccttacat
     3781 ttaacactct taccatttcc aaaatataca aaatatattt tgaacatttg tacaatgatg
     3841 aaatacaata catgaaaata caatatcata actgttgtct aaaaatagaa gttcttcaac
     3901 ataacctttc acaaaaaaca tattatctaa gtattttttg tctgctcctt gttttcctgt
     3961 ttgtagcata taaattactg catctaaaca tataactact cacacgaaaa aagaaaataa
     4021 acttccaaaa tacaagctct aaaaactacc tctaaagttg tataatgttt actaggaata
     4081 aggttgtata acatatagtt taaataatca ttttttaaaa ttgcaagtta ttgctttcaa
     4141 aattagatgt ttttatatca aataaatgat taagaatttc taaatttaat ggctttataa
     4201 tgcactccct ttctttggga taaaataatg ttgcacagat actttttcta aagataacaa
     4261 ttttattttt ctatcaattc caccagcata cccagtcaaa ctcccatttg ttcctacaac
     4321 acgatgacat ggaataataa tagagattcc attatgccct actgcaccac cgattgcttg
     4381 tgctgacatt ttagatattc ctttttgttg agcaacttta tatgcaattt ctccataagt
     4441 gattgtttta ccatatggta tttgttgtaa aattttccac actgtagtct gaaaaggagt
     4501 acctaccata tggagtgatg gtgtaaaatt gggttctttt cccgaaaaat aaatatctaa
     4561 ccatttcttt gtctgctcga aaattggcaa ttctttttct tgatgttctt ggtcaagatt
     4621 ataagcataa tacttttgat tctcaaacca aagaccagtt aacccaatat tatctgatgc
     4681 taaaagaata tttccaattg gactatggta atagctaata tattgcatac aattcctcct
     4741 tatattctga aaacttcact caaatcatta gacacttctt ttctatctac cattatgtaa
     4801 aaacatacta atataatttc cttctaacta cactttcgtt ataatatcta tcctcatatc
     4861 atattagttt tatatttacc tgcatctgtt catccttttt tccctttccc tttgatgaat
     4921 ataaaattca tacatttaag cttcctactg tattaacttt tatagaagtg tatattataa
     4981 ttttatcact aaataggcca aaaaaatatc gatatagcat caagtaatat cataatactt
     5041 tcaaatggga aaagtaagaa taggctgtaa aaaagaaaag tatttttatg ttagaaatcc
     5101 agtaaaatca ttagtagata gaataacaga aaacgaacaa tttgctattt atcattttac
     5161 acataaatat aattgacgca ataaagtgtt ttctattcta caacgattaa taaggttaga
     5221 aaaaagtaaa aatgaactgt atatgtatga agcattagtt tctgtttctt tcctttggct
     5281 tattatgata aaaaatattg ttttgctacc aataaaacac aaaaatataa ttaatatcca
     5341 tatgcaaaaa attttacgtt atattgaaaa atactattgt tttacattat attgaagggt
     5401 actattgtga aaacttaatt ttggagtact atcaagcagt ggtaatatca ataaatcaga
     5461 gtgctccaga tgatttgaac taagtttaaa tactatacct tataaatatt ttactgagtt
     5521 ttgtttatta aaagcaactc aatttctaaa aggcacaaat gagctaataa gtgacattgc
     5581 tataaaagtt gtttttcatc aaataatcta ttttagtaaa tgttttaaag aaaaaacggg
     5641 atattctcca aaagtgtaca gaaatataaa aaaagagata tgatttttta tttcttttga
     5701 ttatccaaag agtaaataaa aaatattgta aaaaacttac taacaacggc aatgctttga
     5761 actatggtaa tactttgaac ttttgattgg actttttttg ctaaaataat atatttaaat
     5821 agcaaagtaa tctgtttaat caataaaaaa taagagataa aagaaatcaa ctaaaacttt
     5881 tacctcttat tttgatagtt tatcagagct aatatttaca ctctttttat ttttgatact
     5941 tcattaaaaa tgatgcttac tctccattca cttttaattc ttcattaaaa atgatgctta
     6001 ttctccattc acttttaatt cttcattaaa aatgatgctt actctccatt cgcttttaat
     6061 tcttcattaa aaatgatgct tattctccat tcacttttaa ttcttcatta aaaatgatgc
     6121 ttactctcca ttcgctttta attcttcatt agaactatgc ttactctcca ttcactttta
     6181 attcttcatt aaaaatgatg cttactctct gttcactttt aattcttcat taaaaatgat
     6241 gcttattctc cattcacttt taattcttca ttaaaaatga tgcttattct ccattcactt
     6301 ttgattcttc attaaaaatg atgcttattc tccattcact tttaattctt cattaaaaat
     6361 gatgcttatt ctccattcac ttttgattct tcattaaaaa tgatgcttat tctctgttca
     6421 cttttaattc tttattaaaa ctgatgctta ttctccattc actttcaatt cttcattaaa
     6481 actgatgctt gcactctgta aactcataaa catccattaa tgcttcacca aatctttgat
     6541 agtgaactac ttccctctca cccaaaaatc tcaatatatc tttcaaatca gggtcatcag
     6601 ttaaatttat taattgatag tacgtagcca atgctttttg ttcagcagcc atatcctcat
     6661 gtaaatcagt taccgaatcg ccaaagacat taatataact tgtagtaaag tttactccat
     6721 tagaatctac tggctgaaga ccatgtccaa aattagcata attactacca agtccagcag
     6781 ccttaagctc ttctggagta caattgccta ttaattgata taacactgaa gaaattatct
     6841 caacatgagc catttcctct gtacctatat cagttaaaag tgcacgagat tttccagttg
     6901 gcatagtata tctttgttga agatatctag ttgcagctgc caactcacca ttaggtcctc
     6961 ccaactgagt catgatatat tttgccattc tagggtctgc ttgtcttatg ttaactggat
     7021 gttccagtgt tttttgataa atccacatat tatcttactc ctccttaata tatatttaat
     7081 aataaatcta attttaaagt ttaataattt tatttataaa atgatttgtc ccatggccaa
     7141 ggttcagata tccatgacca aggatattca cttgattcat atccaaaatt agttaatgct
     7201 ccatatttag attcataatc acacacagct tgtgtaaatt tatcgcagta tctgttgtat
     7261 gaatcaagag ctttcttatc ccttggattg ttatctaaat ataaatttaa ttctatacag
     7321 gcaaattgat attctgaaat tctctctaaa agttctcttc tatttaatga cattagtaca
     7381 tgttaccccc ttttttagct ctaactctat ttttaggatt tgcatatagc caatcattat
     7441 agttatttga ctctattaaa tctaattcag gaaataaagt acctcttttt aatgctgttt
     7501 ttggattgta tgtgtcatca taaatttgac gattgatgta tggtctagca aattcacaac
     7561 aatagtcttg tttatcctct tttaaatatc ccattaatta tcctccttaa tttttagaat
     7621 tttaaaatag cattattttt aaaaattgta tactggttct atattataac tggtagtttt
     7681 aataggtctt taataagatt aaatgattaa ataagtatct ttttttctta ttttacctct
     7741 aaaatttttc taattaatct ttcataaata
//
LOCUS       DAMJVK010000143         7722 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.148, whole genome shotgun sequence.
ACCESSION   DAMJVK010000143 DAMJVK010000000
VERSION     DAMJVK010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7722
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.148"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            993..2351
                     /locus_tag="PC414_003474"
     CDS             993..2351
                     /locus_tag="PC414_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HDF2465840.1"
                     /translation="MKAKSTSKFELDGIPPLKEAIPLGLQHLMAMVIGSCLPAILIAN
                     AAGLNHKMSTLLIQAALVFAGLSTLLQLYPIPLFKGFKLGGGIPVIMGATAMFIGAGV
                     SVASKYGLPVLFGSQIIAGIVIIIIGFGLKKIRFIFTPAVSGTIVACMGLGLFPIAIK
                     NLAGGMGNETFGNPINFIVGISVSLMILGLNKYGKGLFKDASILVSIIFGYILSLILG
                     IVNFSSIQEFTLVALPKPLAFGLDIRLEVVVMFSIIYLVEIADIMGACTLSAVGGLNR
                     QVTDEELSSAVLGNGVLSTIGALFSATPMGMFGQNSAIVSNTKVVSKFVLAIGGIGLF
                     LAGISPLLANLIRTIPPCVVGGATLVIFSTLTTSGLRLVSMDGFNQENSMILGLSMAS
                     GIGFMVAPQVLEKFPKFIETLLADSSVVSGAMVAIIIQALYMVKFPGNKSDKVEDKNK
                     SL"
     gene            2458..3252
                     /locus_tag="PC414_003475"
     CDS             2458..3252
                     /locus_tag="PC414_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HDF2465841.1"
                     /translation="MVTIKEYSVPQSLEEAYKILISKKNNVILGGCGFIKLSNKNIGT
                     AIDLKDINLNYIKEDKKNILIGADTSLRSLEIDKTIKNYCSGILSSAVSNIVGVQFRQ
                     GARIGASVFSKYGFSDLIPALLVVGAKVRLYNKGILELSEFLDSELSRDILVEVILPK
                     KDAIAVFDSIRKCTGDFSVLNSAMLKENDTYKIAIGARPQKARLALEASNILNKEKDI
                     DKASIVASKELTYGSNMRASKEYRKDMASALVKRMYNAIEEGMYND"
     gene            3245..5551
                     /locus_tag="PC414_003476"
     CDS             3245..5551
                     /locus_tag="PC414_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF2465842.1"
                     /translation="MINKGIRKIDSTAIVTGKPLYTEDLIMHKDVLTLKLLRSPHAHA
                     KIKNIDTSKAEKIPGVVGIYTYKDVPQVRYCVPGQTEPEASPYDRVLLEDTVRFIGDE
                     VALVAAEDEKTALKALKMIKVDYEVLKPVFDAREAEGNEVVIHPEKDIVEPYPFGLDA
                     KNNIVSKEFFEFGDVEAKLKESDFVVESSYKTQSQSHCMMETHRSYSYKDINDRIVVV
                     SSLQSPHNVKRILRKVLGIPSSKIRVIKPRIGGGFGGKNIAATDIFTAFVTMKTGRPA
                     KFVFTRSETFVGSNSRHEMFFDVKVGADKEGNLKAIELKSLNNTGAYGGNGVSVSSES
                     GHNTLPIYNDIDAVRFNARTVYTNRLPAGALRGYGATQGIFAIDSAIDELAHKIGMDP
                     LEFRIKNIIDRYTNGKAAEDEILSCELRQCIEKGKKLINWDDKYPCKDIGNNKVRAVG
                     MAVANHGSGIPRVDMATVTIRMDEDGTYKILSGLADLGQGADTIQAQIAAEALNTTVD
                     RISIYTGDSDLCPYDTGAFASCTTTVTGNATIKTANKLKDILLSVAESKLGFKKEDLE
                     LGKDRVFVKNDNSKFVLLEELGRESVGGIGFASDDVPVVLEATESFGMLKKIKPFIAG
                     FAEIELDKNTGEYKVINFACVPDCGTVINPTLARIQVEGGVMMGIGFAMYEDPIFDKD
                     GRLLTDSFLQYKVPTKKEVGNILVDFAENFDPGGPYGAKSLGEIVIHTPAPAIANAIF
                     NATGVRLRELPMTFEKVYMGMKNNNKQL"
     gene            5690..6967
                     /locus_tag="PC414_003477"
     CDS             5690..6967
                     /locus_tag="PC414_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2465843.1"
                     /translation="MSNRIKNIHYGTQRQQLKNLTKKEYQALREMCFLAKNLYNVGTY
                     NVRQHYFNQGEHLNYEANYHVCKENENYKLLNSNIAQQILKEVDSSFRSFFGLIKLAK
                     EGKYDFRGIKSPKYLDKEGFFSIIIGQIRIKEDGILNVPMSPAFKKLYGKVSIKVPQN
                     ILEKKIKEIRIIPKHKARFFEIQYCYEIPKSEEVSNQKNALAIDLGLENLCTCVTNLG
                     DSFIIDGKRLKSVNQWANKQNDKIEALKLKKNITAITRKQFKVWNKRNNQVKDYVNKT
                     CFYIIDYCVKNNIGNLIVGYNAKLEKSDNMERKIYRNFIRIPFGEIKGKLEYLCKIKN
                     INFIRQEESYTSKSDFFADDFLPDINVDNVKKYDFKGERITRGQYKSSVGIIINADVN
                     ASFNILKKSQVVNLSNLQNNPQILKQPQRVRIS"
     gene            7150..>7722
                     /gene="yqeC"
                     /locus_tag="PC414_003478"
     CDS             7150..>7722
                     /gene="yqeC"
                     /locus_tag="PC414_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HDF2465844.1"
                     /translation="MELSNIIEKNEIITVVGAGGKTSFINYFANFYRNKLKVLLTTTT
                     KIYVPNDYDNIIITIDGTVIPSICHGITVCGSYINNENKLVSIDSSILDEIVDQFDLV
                     LIEGDGSKRKKLKGWNAKEPVVYHRTTKTIGILDITSFGMNINEENIHRVEIFKKIAN
                     LDTSSINSSSTVSIENLKNIVLNPNGLFKNY"
BASE COUNT         3027 a          841 c         1440 g         2414 t
ORIGIN      
        1 attttgctta tagatatata tatctataaa tagatagaaa cttgtatatt ttatgttgca
       61 gatacaaata tagtaaagga gagtacattg gtacaactga gatacatagc ttattaagtt
      121 tttctgtctt atgagaatta tattataaaa tattcataaa tattttgagt ttaaattaaa
      181 taattatttt atgcttagat taattgatat ttaattaatt taataacaaa aacagtgaaa
      241 gagtaaataa tattatttac agggggtaaa gcaaagtatg ttaaataaat tgtacttttg
      301 gccccgtaca tagagaaatc tatgtaggac acaactttaa aaccagtaaa tcctaaagcc
      361 ttgcaactac aataaaagat agaaagaaac ttttatgaat gtggtgaaaa ccagaaacaa
      421 tagtgaggat ggtatatggt gagagcctaa gtacttaaaa atggacattt ggtcgccaag
      481 cctagaaata ggaaggtcaa acgactatct cgagagagag tacactataa gcgattgata
      541 gtggaaacgg gttgctctta gcaggtaatg ctgaagatga agaaatagtc tacgctcata
      601 tgaaagtatg aggtcactgg atttgaagtg acatctataa agtagcgttt ataggtaaat
      661 gtttaaaact tgaaacatgt aaatacttat gaaaacttat aacttataat gattaagatt
      721 gaaagattta aattgaatta aatttactat aaaaaattaa aaattcatag ctataatact
      781 cttatatttg taaagcatgg tatataatgt atacaaaatt aaagaatacg tgtatttgtt
      841 aataattaaa tttattgcat ctgtttttta agactaaaaa cttaaatata tgttggagta
      901 tagttgatat ttatgaacat cattaattta atatattaga tttttaagta tttatataat
      961 tcaataagtt ttatgtaacg ggagttttta ttatgaaagc aaaaagtaca tcaaaatttg
     1021 aattagacgg aataccacca ctaaaggaag caataccttt agggttacaa cacttaatgg
     1081 caatggtaat aggtagttgt ttaccagcga tattgattgc aaatgcagct ggacttaatc
     1141 ataagatgtc aacattattg attcaagccg cattagtttt tgcaggatta tcaactttat
     1201 tacaattata tccaatacct ttgtttaaag gttttaaatt aggaggagga ataccagtta
     1261 taatgggagc tacagctatg tttataggag ctggggtatc tgttgcaagt aagtatggac
     1321 taccagtatt atttggctct caaataatag ctggtatagt aataattata attggttttg
     1381 gtcttaaaaa aatacgattt atatttactc cagctgtatc aggaacaata gttgcatgta
     1441 tgggtcttgg actttttcca atagctataa aaaacttagc tgggggtatg ggcaatgaga
     1501 cttttgggaa tccaattaat tttatagttg gaatatctgt atctcttatg atattaggat
     1561 taaataagta tggaaaagga ttatttaaag atgcatcaat attagttagc ataatttttg
     1621 gatacatttt atcattaatt ttgggtatag taaatttttc ttcaattcag gagtttactt
     1681 tggttgcact tccaaaacca cttgcttttg ggctagatat aagacttgaa gttgttgtaa
     1741 tgttttcaat aatatattta gttgagatag ctgatataat gggtgcttgt acactttctg
     1801 cggtaggagg gcttaataga caagtaacag atgaagaact ttcttctgct gttttaggaa
     1861 atggagtact ctctacaata ggtgcattat tttctgcaac tccaatgggg atgtttggac
     1921 aaaattctgc aatcgtttcc aatacaaagg ttgtaagtaa atttgtacta gctattggag
     1981 gaattggatt atttttggca ggaatatcgc ctttattagc taatttaata agaacaattc
     2041 ctccctgtgt ggttggagga gcaacactcg ttatattttc aactcttaca acatctggtt
     2101 taagacttgt tagtatggat ggatttaatc aagaaaatag catgatatta ggtctttcta
     2161 tggcttctgg tattggattc atggtagctc ctcaagttct tgaaaaattt ccaaaattta
     2221 tagagacatt acttgcagat tcaagtgtag tttcaggagc aatggtagcc ataataattc
     2281 aagctttata tatggtaaaa tttcctggaa ataaaagtga taaggttgag gataaaaata
     2341 aaagtttata atgtaaaggt ttaatatatg tgaattaaat atactacaat tataaatata
     2401 aataaaagat ttattaaaaa cttttgtgtt ttaataagtt aatgatgggg ggataagatg
     2461 gttactataa aggaatattc agttccacag agtttagaag aagcatataa aattttaatt
     2521 tctaaaaaaa ataatgttat tttaggtgga tgtggattta taaaattaag taataaaaat
     2581 ataggaacag cgatagattt aaaagatata aatctaaatt atataaaaga ggataaaaaa
     2641 aatattttga taggtgcaga tacttcactt agaagtttag aaattgataa aactataaaa
     2701 aattactgta gtggaatact ttcaagcgct gtttctaata tagttggtgt tcaatttaga
     2761 caaggggcta gaattggtgc aagtgtattt tcaaagtatg gcttttctga cttaatacca
     2821 gcacttttgg ttgttggagc gaaggtgaga ttatacaata aaggaatact agagttaagt
     2881 gagtttttgg atagtgaatt aagtagggat atattggtcg aagtaatact accaaaaaaa
     2941 gatgcaatag ctgtttttga ctcaataaga aaatgcacag gagatttttc tgtattaaat
     3001 tcagcaatgc taaaagaaaa tgatacttac aaaatagcaa ttggggcaag acctcaaaaa
     3061 gctagattag cacttgaagc gagtaatata ttaaataaag aaaaagatat agataaggca
     3121 tctattgttg ctagtaagga acttacttat ggaagtaata tgagagctag taaagagtac
     3181 agaaaggata tggcaagtgc attagttaaa aggatgtata atgctattga ggagggaatg
     3241 tataatgatt aataagggga taagaaagat agatagtaca gcaattgtca caggaaaacc
     3301 tctatatact gaagacctca ttatgcataa agatgttttg acattaaaac tacttagaag
     3361 tccacatgct catgctaaga taaagaatat agatacatca aaagccgaaa aaattccagg
     3421 ggttgtgggt atttatacgt ataaagatgt accacaggtc agatattgtg ttccaggtca
     3481 aacagaacca gaagcatcac cttatgatag agttttactt gaagatacag ttagatttat
     3541 tggagatgaa gtagctttag tggctgctga agatgagaaa acagcactta aagccttaaa
     3601 aatgataaag gtagattatg aggttttaaa gcctgtgttt gatgcaagag aagcagaagg
     3661 aaatgaagtt gttatacatc cagaaaaaga catagtagag ccataccctt ttggattgga
     3721 tgcaaaaaat aatatagtat caaaagaatt ttttgaattt ggagatgttg aagctaagct
     3781 taaagaaagt gattttgtgg ttgaatcttc atacaaaacc caaagtcaat cgcattgcat
     3841 gatggaaact cataggtcat attcatataa agatataaat gatagaattg ttgtagtttc
     3901 atcattacaa tcaccacaca atgtaaagag aatacttaga aaagtattgg gtataccgtc
     3961 ttcaaaaata agagtaatta aacctagaat aggtggtgga tttggaggaa agaatatagc
     4021 agcaacagat atatttactg catttgttac tatgaaaact ggtagaccag caaaatttgt
     4081 atttacaaga agtgaaactt ttgtaggttc taatagtaga catgaaatgt tttttgatgt
     4141 taaagttggt gcagataaag aaggaaattt aaaagcaata gagctaaagt cattaaacaa
     4201 tacaggtgct tatggtggga atggtgtctc tgtttcttcc gaatctggac acaatacctt
     4261 accaatatat aatgatattg atgctgttag atttaatgct agaacagtat atactaatag
     4321 gttaccagca ggagcactta gagggtatgg tgctacacag ggaatatttg caatagatag
     4381 tgctattgat gaattggcac ataagattgg aatggaccca cttgaattta gaattaaaaa
     4441 tataatagat aggtatacca atggaaaagc agctgaagac gaaatattaa gttgtgagct
     4501 gagacaatgt atagaaaagg gaaagaaatt aattaattgg gatgataaat atccatgtaa
     4561 agatatagga aacaacaaag taagagcagt aggtatggca gtagcaaatc atggttctgg
     4621 tataccgcgt gtagatatgg caacagtcac tataagaatg gatgaagatg gcacatataa
     4681 aatattatct ggtcttgctg acttaggtca aggggctgat acaattcaag ctcaaattgc
     4741 tgcggaagct ttaaatacta ctgtagatag aatttctatt tatacaggtg attcagactt
     4801 atgtccctat gatacaggag catttgcttc ttgtacgaca acggttacag gaaatgcaac
     4861 aataaagaca gcaaataaat taaaagatat attactttca gttgcagagt caaagctagg
     4921 atttaaaaaa gaagatttag agttgggaaa agacagggtt tttgttaaaa acgataatag
     4981 caagtttgtt ctattagagg aattgggtag agaatctgtt ggaggaatag gatttgctag
     5041 tgatgatgta ccagtagttt tggaagcaac agaatctttt ggcatgttga aaaaaataaa
     5101 accatttata gcaggatttg cagagataga attagataaa aatacaggtg aatataaagt
     5161 cataaatttt gcatgtgtac ctgattgtgg aacagttata aatccaactt tagctagaat
     5221 acaagtagaa ggtggtgtga tgatgggtat agggtttgca atgtatgaag accctatatt
     5281 tgataaagat ggtagattat taacagatag tttcctacag tataaagttc ctacaaaaaa
     5341 agaagttgga aatatattgg tagattttgc tgagaatttc gacccaggag gtccttatgg
     5401 tgctaaatca ttaggagaaa tagttataca tacaccagca ccagctatag caaatgcaat
     5461 atttaatgct acaggagttc gtttaagaga gcttcctatg acttttgaaa aagtttatat
     5521 gggtatgaaa aataataata aacaattata ataaataata agatgcataa aagcaagtaa
     5581 atttaaatta ttttgtaaaa taaagtaatt aaatggacat tatatatggt gttttgtaaa
     5641 aataccgtat ataatgtcta gtagagtata ttctaaagga ggtgagttca tgtcaaacag
     5701 aataaaaaat atacactatg gaactcagag acaacaatta aaaaacttaa ctaaaaaaga
     5761 atatcaagct ctacgagaga tgtgcttctt agctaagaac ttatataatg ttggtacata
     5821 taatgtaaga cagcattatt tcaatcaagg agaacatctt aactatgaag ctaattatca
     5881 tgtatgcaaa gaaaatgaga actataagct tctcaatagt aatatagcgc aacaaatatt
     5941 aaaagaagta gacagttcgt ttaggtcatt ttttggatta attaaattag ccaaagaagg
     6001 aaaatatgac tttagaggga taaaatcacc taaatattta gataaagaag gattttttag
     6061 cataataata ggtcaaataa gaatcaaaga agatgggatt ttaaatgtac ctatgtctcc
     6121 agcatttaaa aaactatatg ggaaagtaag tataaaagtt ccacaaaata tactagagaa
     6181 aaaaattaaa gaaataagaa taatacctaa acataaagct aggttctttg aaattcagta
     6241 ttgttatgaa ataccaaaat cagaggaagt ttcaaatcaa aaaaatgcac tagctataga
     6301 cttgggttta gaaaatttat gtacatgtgt taccaattta ggtgatagtt ttatcataga
     6361 tggaaaaagg ctaaaatctg tgaatcagtg ggcaaacaaa cagaatgata aaatagaagc
     6421 cttaaaatta aaaaagaata taactgcaat aacaagaaaa caatttaaag tttggaataa
     6481 gagaaataat caagttaaag actatgtaaa taaaacctgt ttttatataa ttgattattg
     6541 tgtaaaaaat aatattggaa atttaattgt aggatataat gctaagttag aaaaaagtga
     6601 taatatggag agaaaaatat atagaaattt tataagaatc ccatttggag agataaaagg
     6661 aaaattagaa tatctgtgta aaataaaaaa tatcaatttt attaggcaag aagaaagcta
     6721 tactagtaaa tcagattttt ttgctgatga ttttctacct gatatcaatg tggacaatgt
     6781 taaaaaatat gactttaaag gggaaagaat aacacgtgga cagtacaaat ccagtgttgg
     6841 aatcattata aatgcagatg taaatgcgag ttttaatata ctaaaaaaat cacaagtagt
     6901 taatttatct aatttacaga ataatcctca aatattaaag caaccacaga gagtaagaat
     6961 atcttaatta tatttaaaag ataaactgat agcgtaagct tacagcttag cggcgaattg
     7021 gatccgcctt gttgtttaca acagaatcta tttactttcg agtttatgga atttcaaaaa
     7081 tcacttttac taaaaaggcc taactataat aattaagaga ttagaaaact ggttataaga
     7141 ggtgaaagtg tggaactatc aaatataata gaaaagaatg aaataataac tgtagttgga
     7201 gcaggaggaa aaacttcgtt tataaactat tttgcaaatt tttatagaaa taaattgaaa
     7261 gttcttctta caacaacaac taaaatatat gtaccaaacg attatgacaa tataattatt
     7321 acaatagatg gaactgttat tccatctatt tgtcatggaa taacagtctg tggcagttat
     7381 ataaataatg aaaataaatt agtaagtatt gattcctcta ttttggatga aattgtagac
     7441 caatttgatt tagttttaat agagggagat ggctcaaaga gaaaaaaact taaaggttgg
     7501 aatgctaaag aaccagtagt atatcataga actacaaaaa ccatagggat attagatata
     7561 acatcatttg gaatgaatat taatgaagag aatatacata gagtagaaat atttaaaaaa
     7621 atagcaaatt tagatacaag tagtattaat agtagttcta cagtaagtat tgaaaactta
     7681 aaaaatatag tattaaatcc aaatggatta tttaaaaatt at
//
LOCUS       DAMJVK010000144         7658 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.149, whole genome shotgun sequence.
ACCESSION   DAMJVK010000144 DAMJVK010000000
VERSION     DAMJVK010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7658
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.149"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            106..1380
                     /locus_tag="PC414_003479"
     CDS             106..1380
                     /locus_tag="PC414_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF2465845.1"
                     /translation="MITISHITAKNMYKSLEERINKFPQGAPPSDTLYKILNVLYTEQ
                     EAKLVAQLPIKPFRVKTAAKIWSVSESEAYRVLDKLASKALILDIEDNKGKKYIMPPP
                     MAGFFEFAMMRTRHDIDQKLLAELYYQYMNVEEDFIKDLFYSTETKLGRVYVQEEVLT
                     NDNEVSILDYERATHIIDESTHIGISMCYCRHRMQHVGKACDAPMDICMTFDNVANSL
                     INNKFARRVDKIECKELLHQAYEHNLVQCGENVRKGVTFICNCCGCCCEAMVAAKRFG
                     NLHPVQTTSFIPNINHENCVKCGKCITACPIDAISKVKEDGKEYIKIDEDRCLGCGVC
                     VRNCHKNSIMLLKRDEKIITPANSVHRAVLMAIEKGQLQNLIFDNNALASHRAMGAIL
                     SAILKLEPAKKILASKQLKSVYLDKLLSMNDK"
     gene            complement(1541..4582)
                     /locus_tag="PC414_003480"
     CDS             complement(1541..4582)
                     /locus_tag="PC414_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp20"
                     /protein_id="HDF2465846.1"
                     /translation="MFKQRLSKLLSSTLVLSMLFTAAPNITFADNTKDNSEKYQSSDI
                     ELHDYSKNAESYTKTKALAKEKIQTLLSKYGAVSAQYALIDNGKIEISGNGGVYSKQD
                     NKNLNKDNMYSIASISKMFTTTAVMKLVDDGKLNLDTPVVKYIPEFKMADDRYKEITP
                     RMLLNHSSGLMGSSFKNTILLADNDSYGHDNFLKELQKQRLKAKPGAFSVYCNDGFTL
                     AEILVERVSGMSFTNFLDKYINNPLNLQNTKTTENSFDSSKLAKAYVPYWEDAVPQDN
                     LNAIGAGGLYSSAENLCTFAQTFMKNSNGILSPASVKAMENKEYLNGLWPEGEDSILG
                     YGLGWDCVNTYPFNQYNLKALTKGGDSLLFHSNLIVLPDENMAVAVLSSGGSSQLNEI
                     IGQEILLSALKEKGKIKEIKPDKTFSKPQQVKMPSSLKENSGLYASSNMIKVDVNDNG
                     TLTVSSPYIENGPEDKYVYIGQDRFVSEKGNSCLKFVKEKNNITYLNMSSYDDVPGLG
                     QTASLYYVAQKIDDNNISNSVKEAWKKRNGKDYYLVDEKYTSQSYMFGSVKATLALSD
                     ETPGYIVNTKIMDENNSNAFIEIPGVIGRDLSDIKLHKENGTEYLSFGTLTYVSEDSI
                     TNLPAEKSFTCELESNGYAKWYKIGDDIANKKIEVNLPQNSAFAVYDDKGVPVNYSLV
                     TKNNRVRLPKGGVIVFLGSPNARFEVTYQDEVNASALTGTDRYETSIKISQAGWENAE
                     NAVLINDSAIADALAATPFAYKKNAPILLTGSSQINEKTLAELKRLKVKNVYVVGGEA
                     SINEKSLDTIKSNNISVSRISGSDRYQTSMNIAKELNNISNISKISVVNGEKGLADAV
                     SIGAVSAQNDMPIILTNENSNITEINNLFKNKKIDKSYVIGGEYTVSKNIESKLQNPQ
                     RISGSTRNETNAKVIKEFYKDSKIDNLYVAKNGMNKQDDLIDGLSVGVLAGKTKSPVM
                     LVGNSLDYNQKELFKTMRFKSVTQIGGNGNENSFKQIKEIA"
     gene            4788..5087
                     /locus_tag="PC414_003481"
     CDS             4788..5087
                     /locus_tag="PC414_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDF2465847.1"
                     /translation="MYKNINKKQLKEMMKNEKDVLLLDVRTKDEFKEYKIENAINISL
                     QELINNIDEIYDYKDKKVVVYCRSGHRSVTACNLLAEEGFEHLYNLNSGIIDYMS"
     gene            complement(5326..7554)
                     /locus_tag="PC414_003482"
     CDS             complement(5326..7554)
                     /locus_tag="PC414_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HDF2465848.1"
                     /translation="MRHLKKTNSLKEILNWNNIKNLRPSKGLKNILEIFKSDLKDIRG
                     NYAVLVIVLGLAFLPSLYAWFNIKASWDPYGSTANMLVAVTNEDKGNEIAGQKINVGD
                     QLIEQLKDNKSLGWQFVDEKTALEGVKKGTYYASLKIPKNFTKDLTSLITDDVKKGEI
                     IYTVNEKINAVAPKITDKGASTIQLQVNQTIVKTVSEIVLGISNGVGAGIEHSLPKLT
                     KVENSLIDLQKRFDHINKTVDLASDATSKLSDTVKDIKSDLPTIKKTLNDTKLLSSDL
                     KKFLEDTNDNLDELSPLIKSDLNLMVDLSSSASSLTLNLIDAVNSGSEDVPKLIDNLS
                     EKLSNLQSLNDTLVDFLTKLNQLTSNNRLDDVIDNLEDSSNKIDSSISTLNDIKNKVI
                     SGQQPSISALNNVLSLSNGIGRINLNILNNFDSKISKPINSIFANSIKVANDIITVLD
                     KAEAKLPKVEEILTTSLKLSGNAQESISLIRERLPLAKGMLDDLIDTLSKISNGEDMK
                     KLVSLLESDILTKSNFLKEPVEVKTEKLYPIENYGSAMTPFYSVLSTWVGILLISALL
                     STSVHGNYKPYEIYFGRGLTFLSIALVQGFIIGTGDILLLGVTAKHPILLIVLLMFTS
                     MVFNFIVYSLVSVFGNVGKAIAIVLLVVQIGSSGGTFPIQVTPTFFQRINPIMPFTYA
                     IGATREAVGGIYAPNLTKDILVLLLFMILSICLNVLLKGPINRVIKPFNDKFGDSNLT
                     GH"
BASE COUNT         2650 a         1098 c         1081 g         2829 t
ORIGIN      
        1 actagaggta tttaactatt tagaaagaat tggtattata taaagtaaga gaatatataa
       61 taagaaagaa tatttgattt tattggaaaa caaaagaaaa agaaggtgat tactatttct
      121 catataactg cgaagaatat gtataaaagt ttagaggaaa gaataaacaa gtttccacaa
      181 ggtgctccac catctgatac attatacaaa atactaaatg tactatatac agaacaagag
      241 gcgaagttag ttgctcagct tccaataaag cctttcagag taaaaacagc agctaaaatt
      301 tggagtgtaa gtgaaagtga agcatataga gtattggata aacttgctag taaggctctg
      361 atactagata tagaagataa taaagggaag aaatatataa tgcctcctcc aatggctggt
      421 ttttttgagt ttgccatgat gagaacgaga cacgatatag accaaaaact tttggcagag
      481 ctttactatc aatatatgaa tgtagaagaa gattttatca aagatttatt ttattcaaca
      541 gaaacgaagc ttggaagagt atacgttcaa gaggaagttt taactaatga taatgaggtt
      601 agtattttag attatgaaag ggctactcat ataattgatg aatcaactca tataggtata
      661 agtatgtgtt actgtagaca tagaatgcaa catgttggaa aagcttgtga tgctcctatg
      721 gatatatgta tgacgtttga caatgtagca aattctttaa taaataataa atttgcaaga
      781 cgtgtggata aaatagaatg taaagagcta cttcatcaag catatgaaca taacttagtt
      841 caatgtggag aaaatgttag gaaaggggtt acgtttatat gtaattgctg tggatgttgc
      901 tgtgaagcaa tggtagcagc taaaagattt ggaaatcttc atccagtaca gacaacaagt
      961 tttataccaa atataaacca tgaaaattgt gtaaagtgtg gtaaatgtat aactgcatgt
     1021 ccaatagatg cgataagcaa ggttaaggaa gatggtaaag aatatataaa aattgatgag
     1081 gatagatgtt taggttgtgg agtttgtgtt agaaattgcc ataaaaatag cattatgtta
     1141 ttaaagagag atgaaaaaat aataactcca gcaaattcag tacatagagc agttcttatg
     1201 gcaattgaaa aagggcagtt acaaaattta atttttgata ataatgctct ggctagtcat
     1261 agagctatgg gagctatact gtcagctata ttaaagcttg aaccagctaa aaagatttta
     1321 gccagtaaac aattaaagtc agtatattta gataaattgt taagtatgaa tgataaatag
     1381 aaactaactt aaaaaaggca aagttttaga cttatctata tcttaataaa cttatcattt
     1441 aatatttaaa tttaattttt acacatataa aacttataac ttaaataaaa aaaggctata
     1501 ttcaattaat attacttgaa tacagccttt tttagttatg ttaagctatt tcttttattt
     1561 gcttaaatga gttttcattt ccatttccac caatttgtgt tacagattta aatctcatag
     1621 ttttaaatag ttctttttga ttataatcta gtgagtttcc tactagcatt acaggagatt
     1681 tagtttttcc agccaaaact ccaacagata atccgtctat taaatcatct tgtttattca
     1741 ttccattttt agcaacatat aaattgtcta tcttagaatc cttgtaaaat tcttttatta
     1801 cttttgcatt agtttcattt ctagtgctac ctgatattct ctgtggattt tgaagtttac
     1861 tttctatatt tttagaaact gtatattctc caccgattac ataagattta tcaatctttt
     1921 tgtttttaaa caagttattt atttcagtta tattactatt ttcattagta agtattatag
     1981 gcatatcatt ttgagctgat acagctccaa tgcttactgc atcggctaat cctttttcgc
     2041 cattaactac tgaaatcttt gatatattgc taatattgtt caattcttta gcaatgttca
     2101 tagaagtttg atatctatca cttcctgaaa ttctagatac agagatattg tttgatttta
     2161 tagtatctaa agatttttca tttatagaag cttctccacc aactacatat acatttttaa
     2221 cttttaatct ctttaattca gccaaagttt tttcatttat ttgagagctt ccagtcaata
     2281 atataggagc gtttttcttg tatgcaaatg gtgttgcagc caaagcatct gcaattgctg
     2341 aatcgtttat taaaactgca ttttcagcat tttcccaacc tgcttgactt atcttaatag
     2401 aagtttcata tctatcagtt cctgtaagtg cacttgcatt tacttcatct tgatatgtaa
     2461 cctcaaatct agcatttgga cttcctaaga aaacaattac gcctcctttt ggcaatctaa
     2521 ctctattgtt ctttgtaact aaagaatagt ttacaggaac acctttatca tcataaacag
     2581 caaaagctga attttgagga agattaactt ctattttttt atttgcgata tcgtcaccaa
     2641 tcttatacca ttttgcataa ccattggatt ctagttcaca agtaaaagat ttttctgctg
     2701 gcaaattagt tatagaatct tcactaacat acgttagtgt tccaaaagat aaatactctg
     2761 taccattttc tttatgaagt ttaatgtcgg ataaatcacg acctattaca ccaggtattt
     2821 ctataaaagc atttgagtta ttctcatcca ttattttagt gttaactata taacctggag
     2881 tttcatcaga aagagcaaga gttgccttca cagaaccaaa catatagctt tgagaagtat
     2941 atttttcatc tactagatag taatctttac catttctttt tttccatgct tcttttacac
     3001 tatttgatat attgttgtca tctatctttt gagctacata gtataaactt gctgtctgac
     3061 ctaatccagg gacatcatca taagagctca tatttaaata tgtgatattg tttttttctt
     3121 ttacaaattt tagacagctg ttaccttttt cagatacaaa tctatcttga cctatgtata
     3181 cgtatttatc ttcaggtcca ttttctatat aaggagaaga tactgttaaa gtaccattat
     3241 cattaacatc tacttttatc atatttgaag aagcataaag accactattt tcttttagag
     3301 agctaggcat tttaacttgt tgtggtttac taaatgtttt atcaggtttt atttctttaa
     3361 ttttaccttt ttcttttaat gcagatagta agatttcttg tccaattatt tcattaagtt
     3421 gactacttcc accagaagaa agaactgcaa ctgccatatt ttcatctgga agaactataa
     3481 gattactgtg gaataataaa gagtctccac cttttgtaag agcttttaaa ttatactgat
     3541 taaatggata agtgttgaca caatcccacc ctaaaccata tccaagtata gagtcttctc
     3601 cttcaggcca tagaccattt aaatattctt tgttttccat agcttttact gaagcaggag
     3661 aaagaatacc atttgaattt ttcataaatg tttgtgcaaa tgtacataga ttttctgcac
     3721 ttgaatataa accacctgca cctatagcat taaggttatc ttgcggaact gcatcttccc
     3781 aataaggcac gtatgctttt gctagttttg aactatcaaa actattttct gtagtttttg
     3841 tattttgtaa gttaagtgga ttatttatgt atttatcaag aaaattagta aatgacatac
     3901 cagatactct ttctactaaa atttcagcta aagtaaatcc atcattacaa taaacggaaa
     3961 aagctcctgg tttagctttt aatctttgtt tttgtaattc ttttaagaaa ttatcatgac
     4021 cataagaatc attgtctgct aataaaatag tgtttttaaa actacttccc atcaaccctg
     4081 atgaatgatt taataacatt cttggagtga tttccttgta tctatcatca gccattttaa
     4141 attcaggtat atactttaca acaggagtat ctaaatttaa ttttccatca tcaaccaact
     4201 tcataacagc tgttgtagta aacattttac tgatagatgc gatactatac atattatcct
     4261 tatttaagtt tttgttatcc tgtttgctgt aaactccacc attaccagaa atctcaattt
     4321 tgccattatc aatcaaagca tattgagcac ttacagcacc atactttgat agcaaagttt
     4381 ggattttctc tttagcaaga gctttagttt ttgtgtaaga ttccgcgttt ttactataat
     4441 catgtaattc tatatcagaa ctttgatatt tttcggaatt atcttttgta ttatcagcaa
     4501 aagtaatatt tggagcagca gtaaataaca tactcaatac taatgtacta gataataatt
     4561 tagagagcct ttgcttaaac atattacatt acctctttcc ctttttaata atttataatt
     4621 agcataacat tcttaattat tttaatcaac agattattat caataaaaaa agcttttata
     4681 agtatagctt tttatataac agagaaaaat ataaaaataa tatttgttgt tgtataaaga
     4741 ataaagggta taggtaatat ataaataaaa tggaggaatt ttagacaatg tataagaata
     4801 taaataaaaa gcaacttaaa gaaatgatga aaaatgaaaa ggatgtttta ttattagatg
     4861 taagaactaa agatgaattt aaggaatata aaatagaaaa tgctataaat atatctttac
     4921 aagagttaat caataatata gatgaaattt acgattataa agataagaaa gtagtagttt
     4981 attgcagaag tggtcataga agtgttactg catgtaattt gttagctgaa gaaggttttg
     5041 agcatttata taatttaaat tcaggaataa ttgattatat gagttaaata atgtaagtta
     5101 tacattaaat actatgtata gctctgctta ttaagtagga atatatctaa atatttataa
     5161 gataaattta ggaaagaacg aattttttcg tttttttcta tgacagtttt tgtagtaaat
     5221 attagttttt aagttggaaa aaggtagtag ctttttagct actacctttt aatatgaact
     5281 tagaatatta ttaaaaaaaa tatatttaaa gacagaatta attttttaat gaccagttaa
     5341 attactatcg ccgaatttat cattaaatgg ttttataact ctatttatag gtccttttag
     5401 aagtacattt aaacaaatag acagaatcat aaatagtaat agaactagta tatctttagt
     5461 tagatttgga gcatatatac caccaactgc ttcccttgta gcacctattg cataggtaaa
     5521 tggcattata gggtttattc tttggaagaa tgtaggtgta acctgtatag ggaatgtacc
     5581 accagaagaa ccaatctgaa cgactaatag tactattgca attgcttttc ctacatttcc
     5641 aaatacagaa actaaagaat acactataaa gttaaatacc atactagtaa acataagcag
     5701 aactatcaat aaaattggat gttttgctgt tactcctaac aataatatat ctccagttcc
     5761 tattataaaa ccttggacaa gagctatact taaaaaagta aggcctcttc caaagtaaat
     5821 ttcataaggt ttatagtttc catgtacaga tgtagacaat agtgctgaaa taagtaatat
     5881 accaacccat gtagaaagaa cactataaaa tggtgtcatt gcagaaccat aattttcaat
     5941 tggatataat ttctctgttt tcacctcaac aggttctttt aagaaattag actttgtcaa
     6001 tatatcactt tcaagcaagc tgactaattt tttcatatct tcaccattac taattttgct
     6061 taaagtatca atcaaatcat ccaacattcc ttttgctaaa ggaagtcttt ctcttatcaa
     6121 tgatatactt tcctgtgcat ttcctgaaag ttttagagaa gtagtaagta tttcttcaac
     6181 ctttggaagt tttgcttctg ccttatctaa aacagtaatt atatcatttg ctacttttat
     6241 actgtttgca aatatactat ttataggttt tgatattttt gagtcaaaat tatttaaaat
     6301 atttaaatta attcttccaa taccatttga tagacttaaa acattattca aagcacttat
     6361 agatggttgt tgtccagata taactttgtt tttaatatca tttaatgttg atatagatga
     6421 atctatttta ttgctagagt cttcaagatt atctattaca tcatccaatc tattatttga
     6481 tgtaagttgg tttaatttag ttaaaaagtc caccaaagta tcgtttaaag attgcaaatt
     6541 agaaagtttt tctgataaat tatctatcaa cttaggtaca tcttcactgc cactatttac
     6601 tgcatcaatt aaatttaatg taagagaaga agcagaagat gaaaggtcaa ccattaaatt
     6661 taagtcagac ttaataagag gagaaagttc gtctaaatta tcatttgtat cctctaagaa
     6721 tttttttaag tcacttgata atagttttgt atcattcaga gtctttttaa ttgtaggtaa
     6781 atcagattta atatctttaa ctgtatcaga taattttgaa gtagcatcag aagctaaatc
     6841 aacagtctta tttatatggt caaatctctt ttgaaggtca ataagggaat tttcaacttt
     6901 tgtcaatttt ggaagactgt gttcaattcc tgcaccaact ccgtttgata taccaagaac
     6961 gatttcacta actgttttta ctatagtctg attaacctgt agttgtattg tagaagcacc
     7021 tttatctgta atctttggtg caactgcatt tattttttca ttaacagtat agataatttc
     7081 gcctttttta acatcatctg taattaaaga tgtcaaatct tttgtaaaat tttttggaat
     7141 cttaagtgat gcataataag tacctttttt aacaccttca agagcagttt tttcatctac
     7201 aaactgccaa cctaaactct tgttatcttt gagttgttca attaattggt ctcctacatt
     7261 aatcttttgt cctgctattt catttccttt atcttcattt gttactgcta caagcatatt
     7321 agcagtgctt ccataagggt cccaagatgc ctttatattg aaccaagcat acagagatgg
     7381 taaaaaagca agacctaaaa ctattacaag tacggcataa ttgccacgta tatcttttag
     7441 gtcactttta aaaatttcta atatattttt taaacccttt gaaggtctta aattcttgat
     7501 attattccag tttaaaattt cttttaaact attagttttt ttcaaatgtc tcacctccta
     7561 aaatattttg ataaagaaaa aagttagatt tataatcaat tttaaattat attacttttt
     7621 acacaaatag taaagttaaa attttgtctt aattttaa
//
LOCUS       DAMJVK010000145         7633 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.150, whole genome shotgun sequence.
ACCESSION   DAMJVK010000145 DAMJVK010000000
VERSION     DAMJVK010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7633
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.150"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(74..286)
                     /locus_tag="PC414_003483"
     CDS             complement(74..286)
                     /locus_tag="PC414_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2465849.1"
                     /translation="MAILMKTKIKEYREKLLMTQNELAKLVGVRRETIVHLENGKYNP
                     SLKLAMDIAKVFDTTVENLFEFIEEE"
     gene            complement(287..703)
                     /locus_tag="PC414_003484"
     CDS             complement(287..703)
                     /locus_tag="PC414_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465850.1"
                     /translation="MSKSETGVRSKLILELLVITYFFIAVLATKYIFKGPSTASLPGL
                     SWLIVFGCYSIYNKKYNDLVDESSEFILSKVNKIAIKIMIYSIVIISIFFATPYTSNI
                     NISNLDTGIILIAVLFIQSLSKLVLYIYFDKRGICN"
     gene            complement(771..1184)
                     /locus_tag="PC414_003485"
     CDS             complement(771..1184)
                     /locus_tag="PC414_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465851.1"
                     /translation="MSKSELGVKTNLVSELLMISYFFIDSLLKKFIFKEGNLCITFII
                     LFAFWACDFMYNKKYNDLVDEASKLMLLKVDHIASKIMLYSILLIAVFFSANYTRGLD
                     ISNLDIGVILLAILFIQSLSKLILYIHFDRKGICN"
     gene            complement(2017..2646)
                     /locus_tag="PC414_003486"
     CDS             complement(2017..2646)
                     /locus_tag="PC414_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HDF2465852.1"
                     /translation="MKNRKVYMYVFDTMSDWEVAYLTAELNTGRYFKKGLKPLKVITV
                     GIDKNPIITMGGLKVLPEITVDEFNMESKDLLILPGGNTWLSMNHEIILEKVKEALRQ
                     DSIVAAICGATLGLAKKGLLDFRNHTSNDLEFLKMVIPNYSGEKYYKTESVVSDENLV
                     TASGIAPLEFTFQVLKLLDVFESEALNSWLNLYKTHDSKYFFELMNCNK"
     gene            complement(2725..3687)
                     /locus_tag="PC414_003487"
     CDS             complement(2725..3687)
                     /locus_tag="PC414_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017561613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HDF2465853.1"
                     /translation="MAKIDNMMSILWMLNSDKKITAKQIAEKLEINIRTVYRYIDVLS
                     ASGVPIISDTGHNGGYSLLNNFTKAPLFFDIEEQKSLLHAALFAKKEGYFLDEALDKS
                     ILKLKMYSNQKQEDILTNHLRALEVIKPIGKVSIESVLKKLENSILKELSVEINYHAG
                     RKVKSKYRIINPYGIVHWNNNWYVVAFCNMRNEVRSFRVDRISEIIETSNTFNRPTEF
                     SASEFFIKGIIPEIKDEQNIIQLVIEGSIDSLDSLSQHWFLGHYLKERTPNKIIFMLE
                     EEVICKYIPNILLPYRKTIQVIEPLSLRKKIIEDLLELIDYYQI"
     gene            complement(4449..>7633)
                     /locus_tag="PC414_003488"
     CDS             complement(4449..>7633)
                     /locus_tag="PC414_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="collagen-binding adhesin CpbA"
                     /protein_id="HDF2465854.1"
                     /translation="TNAGDAEGDTNAGDAEGDANAVVVEEEATAEEPTSVGDFKISGG
                     EINVDFKFASGVLTILSGKQMTIQNNNPNTPTTHRIVIPKDVKAELIFDGVNIKTASN
                     SPFTLTPDKDGGAYAHVILQDGSENKLESGDVYYPGMRAGKGTTLIIDDSVVNRDIEG
                     EFIVPEGGKIPRDVTLENGDKLKKGDRLTLLDSNNRGSLKVVGNYYSAGIGGGNTESG
                     GNIIINGGAITAIGQESSRNTGYGAGIGGGGNGSGGDITINGGIITAKGAYHGAGIGG
                     GYYTTTKNALSPSVVDPSTGSGYSGNITINGGLSYSYGGAHGSAFGDGCVNPGTNRGY
                     RILVTGGTILPYMTGVGDWIFDLSATTGDVIVTGGSLKATTFKSLGGAVAYGDLEKKT
                     KVFMNKISLTYLGQDKVSTTLVDDFKMLIDGKIYPYGAPSYTDAQGILYLWFSDQFKG
                     SEVSVNLVVIDKITGEVLKPDDFYIKDIGSGSQFLKQYEEFTIDDTPPYLIIKRYDGL
                     PFEEDVEKSFLDWIFKDGGIPIITPKGEKLTDKKFMTISSQRLNEETMEPDINLGSDD
                     LTKQVDAGKYQLSITSTQFSSIYPFKDAYWGHRAYYKAEIIPADTETSLVVEESKAKT
                     DVFKPTDTFTLNVTISPDKKEGPDCASPRGYVQFYINGKEYKDPVELKTHNKGSESHN
                     YSTASIEWRPLDSNHHIFTTEQTITVKYIGDDINYIEGSEDEKKLKLDMTNVDVDGDG
                     IPDINIDTDGDGRPDINIDTSGDWKPDINIDTDNTGEWKPSTEGGNGDGIWKPDKNID
                     TNGDGNPDTDYNRPAIDTDNDGVDDYWKPDKNVDTGSGGYDTGNPNLNKPDPDNGGNS
                     KPDSGNGGNSKPDSGNGGNSKPDSGNGGNSKPDPDNGGNSKPDPDNGGNGKPDPDNGG
                     NSKPDPDNGGNSKPDPDNGGNSKPDIDTDGDGKPNINIDTDGDGKPDINIDTDGDGKP
                     DINIDTDGDGKPDINIDIDGDGKPDINIDTDGNGKPDINIDTDESLDSNVGQNVQTGD
                     QSNIMLDLALMFISLFFLIKNLTNKYLRRK"
BASE COUNT         2474 a         1374 c          809 g         2976 t
ORIGIN      
        1 atatttttaa caaacttaat aattaaataa ctatatttta ttatccatct tataaatgtt
       61 tataaacttt ccactattct tcttcaataa attcaaacaa attttctact gtagtatcga
      121 atactttagc gatatccata gctaacttca aagaaggatt atatttccca ttttctaagt
      181 gtacaatagt ctcacgtctt accccaacca atttagcaag ttcattttgc gtcataagga
      241 gtttttctct gtattcttta atttttgttt tcatcaatat tgccacttag ttacatatcc
      301 cccttttatc aaagtatata tacaacacta atttagataa agactgtata aataaaactg
      361 caatgaggat tatacctgta tctaggtttg atatatttat attacttgta taaggtgttg
      421 caaagaaaat agatattatt actatagaat atatcattat ttttattgca atcttattaa
      481 cctttgatag tataaattca gatgattcat caactaaatc attatatttt ttattataaa
      541 ttgaataaca tccaaaaact attaaccaac tcaaccctgg aagagaagca gtgcttggac
      601 ctttaaaaat atacttagtg gctaatacag ctataaaaaa ataagttatt acaagcaact
      661 ccaggataag ctttgatctt acaccagttt ctgatttaga catataaaat cactccttaa
      721 aaattatatt ctctatactc tttaattttc gtttttatca atattatcac ttagttgcat
      781 attccctttc tatcaaaatg tatatacaaa attaatttag ataaagattg tataaataaa
      841 atagctaaga gaattacacc tatatccaaa tttgatatat ccaaacctct tgtataattt
      901 gctgaaaaga aaacagctat tagcaaaata gaatataaca ttatttttga tgcaatatga
      961 tcaaccttta ataacataag ttttgacgcc tcgtcaacta aatcattata ttttttatta
     1021 tacatgaagt cacatgccca aaaagcgaat aaaattataa aagtaataca aagatttccc
     1081 tctttaaaaa taaacttttt taataatgag tctataaaaa aataggatat cataagtaac
     1141 tctgaaacaa gatttgtttt aacaccaagc tctgatttag acatataaaa tcacccctta
     1201 aaagttatat atttataaca ccttatgtta taaatatata acttttccaa ataaattgca
     1261 atagaatatt gaaaatattc aataatatct tcataaatat attttataaa agattttata
     1321 ataaacatta caatacagta ctaaattagt ttatttcatc tatcatctca atcatatcat
     1381 ttactaacta ttattctaat gagcatataa cattgaaact tataattata caacctaaat
     1441 gcctcccaat gtaccctcaa aacatcattt cctgtaactg aagtataatt ggtatatttt
     1501 atcactgaaa taaaaaaaca ctagtcaata aagactagta ttttcaagta ttttatcatt
     1561 ctttttttaa ttattggaga cgaatcatca gaataatgcc cataatttat ctatatgcaa
     1621 attctaaatt taaaataaag tttcagataa atttactatt ctatgctatg atacaacaga
     1681 aaaaactgaa atctatgagt ggtagcttca tataatccta gttcctacag taatcaaaac
     1741 tgaggtttat tctatatgta actaatgata aattttatta taggagttat tagatctatt
     1801 tgtgttacta taagtagtaa ttatatatat gataattttt taacactttt ctgttggcta
     1861 tatagaaagt ggtttagaat ttaaatttaa ataaaattta aattcaccaa aaagaaatct
     1921 atattagaaa ccactactct attccaccag attgtaattt tttgttcttt atatcacaat
     1981 gctataccat attaaattga aaaataagtt ttaaatttat ttgttacaat tcattaattc
     2041 aaaaaaatat tttgagtcat gagttttgta aagatttagc catgaattta atgcttctga
     2101 ttcaaaaaca tctaaaagtt tcaatacttg aaacgtaaat tccaacggag caattcctga
     2161 agccgtaact aaattttcat cactaactac agattccgtt ttataatatt tttctccaga
     2221 gtaatttggt attaccattt taagaaactc taagtcatta cttgtatgat ttctaaaatc
     2281 taatagccct tttttagcta ggcctaatgt agctccacat atggctgcaa ctatagaatc
     2341 ttgtcttaaa gcttctttaa ctttttctaa tattatctca tgattcatag ataaccaagt
     2401 atttccccct ggcaaaatta ataaatcctt actttccata ttaaactcat caactgtaat
     2461 ttcaggtaaa acttttaacc cacccattgt tataatagga tttttatcaa ttcctacagt
     2521 aattactttt agtggcttta atcctttttt aaaatatctt cctgtattta attcagcagt
     2581 taaatatgcc acttcccaat ctgacatagt atcaaataca tacatatata cttttctatt
     2641 tttcatcttt tttactcctt atgtaattaa tttatatatc taattataaa ataacttaac
     2701 tgacatattc agtcagttaa gctattaaat ttgataataa tcaattaatt ctagtaaatc
     2761 ttcaattatt ttcttcctaa ggcttagtgg ttcaattact tgtattgtct ttctataagg
     2821 taaaagtata tttggtatgt acttgcatat tacttcctct tcaagcataa atattatttt
     2881 attaggtgtt ctttctttca agtagtgccc taaaaaccaa tgttggctta aactatctaa
     2941 agaatctata ctcccttcta taactaattg aattatattt tgttcatctt ttatttcagg
     3001 tataattcct tttataaaaa attcacttgc tgaaaattct gtcggtctgt taaatgtatt
     3061 tgatgtttct atgatttcac taatcctatc cactctgaaa ctacgaacct catttctcat
     3121 attacaaaat gcaactacgt accaattatt attccagtga acaattccat atggatttat
     3181 aattctatat tttgatttta ctttacgacc tgcatgatag ttgatttcta cagatagctc
     3241 ctttaatata ctattctcca attttttaag tactgattct atagacactt tcccaattgg
     3301 ttttattact tcaagggctc ttaagtggtt tgttaaaata tcttcttgtt tttgatttga
     3361 atacattttt aactttaata tcgatttatc taatgcttcg tctaagaagt atccttcttt
     3421 tttagcaaat agtgctgcat gaagtagaga tttttgctct tctatatcaa aaaaaagagg
     3481 tgctttagta aaattattta aaaggctata cccaccatta tgacctgtat ctgaaattat
     3541 aggtactcca ctcgcagaaa gtacatcaat atatcggtac actgttctta tatttatttc
     3601 taatttttca gctatctgtt ttgctgtaat ttttttgtca gagtttaaca tccacagtat
     3661 agacatcata ttgtcaattt tagccatgta gtttcacctc tttctatttg ttatataaag
     3721 ttattatata actttttgat tgatttttta caaaaaaaca ttagtaaact gctaataatt
     3781 tatactaaga tttatttcaa atatttatag atagtctttt tctatagtta agttatccat
     3841 taaattacat agtagtatat ctattttagt tgatatattt tcagctacct cccttgaata
     3901 ttattacaat aagtctaaat cttctttctt ccataaatat attatgagga aagactatca
     3961 tctttaatgg tttaatagta taaaagatat taatttaaaa aaatctattt ttatatcaga
     4021 tgggtattgt tttttataaa actgaaaaaa tcacctaata gaatctatga ttttttaaca
     4081 taacctccat aaattagttc caattatcac ttcaaattca aatttttatc tgaagaatat
     4141 acaatcatat ttccatactc aacataatat tgagtgtttg gcttgtattt acaaattatt
     4201 attctaacaa aaacaactat acatagatta aaatcatctt ctaaacataa ctactaaaat
     4261 tgaattaatg atatcaattc cattaatctc ttttaataaa aaattctctt ttctatatga
     4321 aaattatttc aaaactttta agttaaatac aaaaatgact ataaatagat ttttatttac
     4381 acaaatctat ttatagtcac tatagtttat ccaataaaat aataaaataa atatgtacac
     4441 aataaatgtt atttacgtct taagtattta tttgttagat ttttaattaa aaaaaacaag
     4501 ctaatgaaca ttaatgctaa atctagcatt atattgcttt gatctcctgt ttgtacattt
     4561 tgacctacat tactatctaa actttcatct gtatctatat ttatatctgg ttttccattc
     4621 ccatctgtat ctatatttat atccggtttt ccatctccat ctatatctat atttatatct
     4681 ggttttccat ctccatctgt atctatattt atatctggtt ttccatctcc atctgtgtct
     4741 atatttatat ctggttttcc atctccatct gtgtctatat ttatattcgg ttttccatcc
     4801 ccatctgtat ctatatctgg tttgctattt cctccattat ctgggtctgg tttactattt
     4861 ccaccgttat ctggatccgg tttgctattt cctccattat ctggatctgg tttaccattg
     4921 ccaccgttat ctggatctgg tttgctattt cctccgttat ctggatccgg tttgctattt
     4981 cctccattac ctgaatccgg tttgctattt cctccattac ctgaatctgg tttgctattt
     5041 cctccattac ctgaatctgg tttgctattt cctccattat ctggatctgg cttatttaga
     5101 tttggattac ctgtatcata acctcctgag ccagtatcta catttttatc aggcttccaa
     5161 taatcatcta ctccatcatt atctgtatct attgctggac gattataatc tgtatcaggg
     5221 tttccatctc cattggtatc tatattttta tcaggtttcc aaataccatc tccatttcca
     5281 ccttctgtac taggcttcca ttcacctgta ttatcagtat ctatgttaat atctggcttc
     5341 caatcaccac tcgtatctat attgatatct ggccttccat caccatcagt gtctatattt
     5401 atatccggta ttccatcccc atctacatct acatttgtca tatctaactt taacttcttt
     5461 tcatcctcac taccttcaat gtaatttata tcatctccaa tatattttac agtaatagtt
     5521 tgttcagtag taaaaatatg atgattgcta tctaaaggtc tccattcaat agatgcagta
     5581 ctataattat gactctcact acccttatta tgagttttta attctactgg atctttatat
     5641 tcctttccat taatatagaa ttgtacgtaa cctcttggag aggcacaatc tggtccttct
     5701 ttcttatctg gtgaaatagt aacatttaat gtaaacgtat ctgttggttt aaaaacatca
     5761 gttttagctt tagactcttc tacaactaat gaagtttctg tatcagcagg aataatttct
     5821 gccttataat atgctctatg cccccaataa gcatccttaa aaggatatat actcgaaaac
     5881 tgtgttgatg taatgcttaa ttgatatttc ccagcatcaa cttgttttgt aagatcatca
     5941 cttcctaaat ttatatctgg ttccatcgtt tcctcattta aacgttgaga actaatagtc
     6001 ataaattttt tatctgtgag tttttcacct ttaggagtaa ttattggaat accaccatct
     6061 ttaaatatcc aatctaaaaa tgatttttct acgtcttctt caaatggtaa tccatcatat
     6121 cttttaataa tcaaatatgg tggagtatca tcaatagtaa attcttcata ttgctttaaa
     6181 aattgagaac ctgaacctat atctttaata taaaaatcat ctggttttag aacttctcca
     6241 gtaattttat ctataacaac taaattaaca gatacttcag atcccttaaa ttgatcagag
     6301 aaccataagt aaagaatccc ttgagcatct gtataactag gtgctccata aggataaatt
     6361 tttccatcaa ttagcatttt aaaatcatca actagtgtag tactaacttt atcttgccct
     6421 aaatacgtta gagatatttt attcataaaa acttttgttt tcttttctaa atcaccgtat
     6481 gcaactgctc cacctaaact tttgaatgtt gttgctttta atgaaccacc tgttactata
     6541 acatctcccg ttgtagcact taggtcaaaa atccaatctc ccactcctgt catataaggt
     6601 aaaatcgttc ctccagttac aagtatacga tatcctctat ttgttcctgg attaacacat
     6661 ccatccccaa atgcactacc atgtgcacct ccatatgaat aggaaagtcc accattaatt
     6721 gtaatattcc cactataacc agaacctgtg ctaggatcta ctactgaggg acttaatgca
     6781 ttctttgttg ttgtatagta tcctccacct attccagctc catggtatgc acctttagca
     6841 gttataatac ctccattgat tgtgatatct ccaccactac cattaccgcc tccacctatt
     6901 ccagctccat atcctgtatt acgactcgat tcttgaccta tagcagttat agcaccacca
     6961 ttaataataa tatttcctcc actttcagta tttccaccac caatacctgc agaataataa
     7021 tttccaacaa cttttaaact tcctctatta ttactatcta gtagtgtaag gcgatctcct
     7081 ttttttaact tgtctccatt ttctagagtt acatctctag gaatttttcc accctcagga
     7141 actataaact ctccttcaat atctctattt acaacagaat catcaataat tagagtagta
     7201 ccttttcctg ctctcatacc tggataatat acatcacctg actctagctt gttttcacta
     7261 ccatcttgta atataacatg tgcatatgca ccaccatctt tatctggtgt taaagtaaat
     7321 gggctattag atgcagtttt aatatttact ccatcaaata ttaattcagc ttttacatct
     7381 ttaggtataa caatcctatg tgttgttgga gtatttggat tgttattttg aattgtcatt
     7441 tgttttccac taagaatagt taaaacgcca cttgcaaatt taaaatcgac atttatttct
     7501 ccaccactaa ttttaaagtc tcctacagac gtaggctctt ctgcagtggc ttcttcttct
     7561 acaactactg cattagcatc tccttctgca tctcctgcat tagtatctcc ttctgcatct
     7621 cctgcattag tat
//
LOCUS       DAMJVK010000146         7615 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.151, whole genome shotgun sequence.
ACCESSION   DAMJVK010000146 DAMJVK010000000
VERSION     DAMJVK010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7615
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.151"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            18..92
                     /locus_tag="PC414_003489"
     tRNA            18..92
                     /locus_tag="PC414_003489"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:50..52,aa:Gly,seq:gcc)
     gene            117..193
                     /locus_tag="PC414_003490"
     tRNA            117..193
                     /locus_tag="PC414_003490"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:151..153,aa:Arg,seq:tct)
     gene            203..278
                     /locus_tag="PC414_003491"
     tRNA            203..278
                     /locus_tag="PC414_003491"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:236..238,aa:Gln,seq:ttg)
     gene            287..362
                     /locus_tag="PC414_003492"
     tRNA            287..362
                     /locus_tag="PC414_003492"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:320..322,aa:Lys,seq:ttt)
     gene            365..453
                     /locus_tag="PC414_003493"
     tRNA            365..453
                     /locus_tag="PC414_003493"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:399..401,aa:Ser,seq:tga)
     gene            457..532
                     /locus_tag="PC414_003494"
     tRNA            457..532
                     /locus_tag="PC414_003494"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:490..492,aa:Phe,seq:gaa)
     gene            539..612
                     /locus_tag="PC414_003495"
     tRNA            539..612
                     /locus_tag="PC414_003495"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:573..575,aa:Met,seq:cat)
     gene            627..703
                     /locus_tag="PC414_003496"
     tRNA            627..703
                     /locus_tag="PC414_003496"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:661..663,aa:Ile,seq:cat)
     gene            721..797
                     /locus_tag="PC414_003497"
     tRNA            721..797
                     /locus_tag="PC414_003497"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:755..757,aa:His,seq:gtg)
     gene            806..881
                     /locus_tag="PC414_003498"
     tRNA            806..881
                     /locus_tag="PC414_003498"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:839..841,aa:Lys,seq:ttt)
     gene            889..962
                     /locus_tag="PC414_003499"
     tRNA            889..962
                     /locus_tag="PC414_003499"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:921..923,aa:Cys,seq:gca)
     gene            970..1046
                     /locus_tag="PC414_003500"
     tRNA            970..1046
                     /locus_tag="PC414_003500"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1004..1006,aa:Arg,seq:acg)
     gene            1059..1134
                     /locus_tag="PC414_003501"
     tRNA            1059..1134
                     /locus_tag="PC414_003501"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1092..1094,aa:Val,seq:tac)
     gene            1210..1623
                     /locus_tag="PC414_003502"
     CDS             1210..1623
                     /locus_tag="PC414_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465855.1"
                     /translation="MYIVIGNEIVDSEELKIIIDKNSEFKVEKDLSKSTKREDVIAYQ
                     LSIDLNYLDSLINEQCNLASLSDEEKFDEYMTLSDELALDLEELMPKYTIINARAYKL
                     DEVDGIVKIILAVAYADLGHLKLSDVVKRLSRQVD"
     gene            1932..5363
                     /locus_tag="PC414_003503"
     CDS             1932..5363
                     /locus_tag="PC414_003503"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HDF2465856.1"
                     /translation="MLKKFNKILVANRGEIAIRIFRACSELGIKSVGIYSKEDKYGLF
                     RTKADESYLIGEGKGPIDAYLDMDGIIDLAKRKKVDAIHPGYGFLAENAEFARKCEEN
                     GITFIGPSSKVMNMMGDKINSKKIAKEVNVQTIPGVEKAIRSTEEAKEVANKIGYPVM
                     IKASNGGGGRGMRIVHREEDLELEYETACSESRKAFGEDIIFIEKYIADPKHIEVQIL
                     GDNYGNIVHLYERDCSVQRRHQKIIEYAPAFSLDDKVRKEICEDAVKLSKHVGYSNAG
                     TLEFLVDANGGHYFIEMNTRVQVEHTVTEMVTGIDIVQSQILIAQGYSLDSEEINIKS
                     QDDVEIRGYSIQCRITTEDPKNKFMPDTGKIQVYRTGSGFGIRLDGGNGFTGANISPH
                     YDSLLVKTISWDRTFQGAINKTIRSIKELRVRGVKTNVGFLVNVLNNPIFSNGKCSTK
                     FIDENPDLFEITESKDRGTKLLQFIGDVIVNDNACKEKPLFDALHDPRMDKDGSKSEG
                     SKILFDKLGKSAYIEKIKNDKKLLLTDTTMRDAHQSLLATRIRTYDLLKAAKPTEKYQ
                     KDLFSLEMWGGATYDVAYRFLKESPWRRLQKLREEIPSIMFQMLLRASNGVGYKNYPD
                     NVIEEFTRESARQGIDVFRIFDSLNWVENMKPSINTALETGKIVEATMCYTGDILDKT
                     KTKYNLEYYIKMAQELESLGADIIAIKDMSGLLKPYSAYTLVKELKKNVKAPIHLHTH
                     DTSGNGVATCLMASEAGVDIIDAALESMAGLTSQPSLNAIVEALKNTERDTGIDLFGY
                     DELGKYYKDLRKVYNKFESDLTNSCAEIYNFEIPGGQYTNLKPQADSLGLVNRFDEVK
                     EKYKEANEVVGDIIKVTPSSKVVGDLAIFMTKNKLDKDNIIEEGKNLSFPDSVVDYCK
                     GMIGQPEGGIPKDLQEVVLKGEEAITVRPGSLLPAEDFDEIAKYLNEKYDINANIRNV
                     ISYALYPKVYEDYIKHLQHYNDISKLESDVFFYGLNKNEECEVEIEEGKVLTIRLVEI
                     GEVKENGFRTIGFELNGMVREVEIKDKNFSGKINNVEKADMNDPLQIGASIPGKVIKI
                     MVKEEDEVKANQPLIVIEAMKMETIIVAKTDGVIKSIKVKEDDMVEDKQLLMIMK"
     gene            5605..7533
                     /locus_tag="PC414_003504"
     CDS             5605..7533
                     /locus_tag="PC414_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDF2465857.1"
                     /translation="MKVYDSKMLRNVAVLGHSGCGKTNLIETIAYTANTNKIPKLTDK
                     VNMTYSMGLIPIEYNDYKFNLLDTPGYFDFSGDVVSSLRASDAAIIVIDATAPIQVGT
                     EKSLELTESIPKIMFINKIDNEKARYKDAIAMLREKYNNKIVPMISPIYKDKNFVKLH
                     NVFENIDDLEGEFKEQAMSVKEALMELIAETDDQILDKYFNGEELTTEEIQKGIIIGI
                     QRGDIIPVICGSTINNIGTKEILDTISSYLEPIFTEESKPFRGLVFKTMVDPFVGKMS
                     YIKITEGVLSKDKDVFNINKNVKEKIANIYTLRNSELVEIEKAKAGDIVVITKVNSLK
                     TGDTISADKDAEALEKIDFPKPQIYYAVTPKNKGDEEKVASVLNKLVEEDPTLHWYRN
                     TETKQALLGGQGELHIKTIKNKMKDKFGVDVELNDLKVPYRETIKGTADVQGKHKKQS
                     GGHGQYGDVKIRFERCESDFEFTEEIFGGSVPKQYIPAVEKGLKDSMQKGILAGYPVT
                     NIKATLYDGSYHDVDSSEMAFKMAASAAFKKGMEEAHPILLEPIMKLKITVPEEYMGD
                     VMGDINKRRGKIFGMEPDDKGKQIIFAEAPQAETFKYAIDLRAMTQGRGYFEMELERY
                     GEVPSQFAEKIIGLATAK"
BASE COUNT         3023 a          935 c         1517 g         2140 t
ORIGIN      
        1 tttaatttaa taaacgagcg ggaatagttc agtggtagag cgcaaccttg ccaaggttga
       61 agtcgcgagt tcgaatctcg tttcccgctc caaattttat gtttatgttg taatgtgggt
      121 gcatagctca gctggataga gcaacgccct tctaaggcgt gtgtccgggg ttcgaatccc
      181 tgtgcgctca ccacatatat attggggatt agccaagtcg gtaaggcaca tgactttgac
      241 tcatgtatgc gtaggttcga gtcctgcatc cccagccaaa cagtatggtc tattagctca
      301 gtcggtagag cacctgactt ttaatcaggg tgtcccgcgt tcgagtcgcg gatagatcac
      361 caatggagag gtgtccgagt ggtttaagga gctggtcttg aaaaccagtg acttcgaaag
      421 gggccgtggg ttcgaatccc accctctccg ccaaatgccc agatagctca gtcggtagag
      481 caggggactg aaaatccccg tgtcggtggt tcgattccgc ctctgggcac caaaatgtgg
      541 cggtatagct tagttggcta gagcgttcgg ttcatacccg aaaggtcata ggttcgactc
      601 ctattaccgc tactgaaatt taatgtgggc cattagctca gttggttaga gcgcccggct
      661 cataaccggt aggtctgggg ttcgagtccc tgatggccca ccacattaaa ttaacttatg
      721 gtgggtatag ctcagttggt tagagcgcca gattgtggct ctggaggtcg tgagttcgac
      781 tctcattatc caccccaact tatatggtct attagctcag tcggtagagc acctgacttt
      841 taatcagggt gtcccgcgtt cgagtcgcgg atagatcacc aaaaaggcgg cgacatagcc
      901 aagtggtaag gcagtggact gcaactcctt gatccccagt tcgaatctgg gtgtcgcctc
      961 caataaaatg cgctcatagc tcaactggat agagtgtctg actacgaatc agaaggttag
     1021 gggttcgagt ccctttgggc gcaccatatg aatagcgagg gattatagct cagctgggag
     1081 agcacctgcc ttacaagcag ggggtcacag gttcgagccc tgttaatccc accagtgcaa
     1141 acctctagta aaactactag aggttttatt tttgattgaa aaagttttta tatttaggag
     1201 gtaaaaaatt tgtatatagt aataggaaat gaaatagttg atagtgaaga acttaaaata
     1261 attatagata aaaatagtga atttaaagtt gaaaaagatt taagtaaaag cacaaaaaga
     1321 gaagatgtaa tagcatatca gttaagtata gatttaaatt acctagattc tcttataaat
     1381 gagcaatgta atttagccag tctatctgat gaagagaaat ttgatgaata tatgactttg
     1441 tctgatgaac ttgcactgga tttagaagag ttaatgccaa aatatacaat aataaatgct
     1501 agagcatata aattagacga ggtagatgga attgtaaaaa taatactagc tgtagcgtat
     1561 gcagatttag ggcacttgaa actttctgat gtagtaaaga ggctatctag acaagtagat
     1621 taggatatga atatgaacta tattaaaaaa atgtataaaa tcttacgtaa agatgaaaat
     1681 aattatgtaa acatagaaaa aaacagtata aaatcaagtc aattgtagaa attttcacat
     1741 aaattcatct gaaattctat aaaaaatgcc ttaattttaa tgaggtaaat cgagtttaca
     1801 cagtctaaaa attacttata tttataaatt tatcttatta attaaggaac tttcttaaac
     1861 atcttcaaaa ataaagtatt ttagtctata atctttaata ttacacaaac aataaaggag
     1921 aagaatagag tatgcttaaa aagtttaata aaatactagt ggctaataga ggcgaaatag
     1981 ctataagaat atttagagcc tgttcagaat tggggataaa aagtgttggt atatacagta
     2041 aggaagataa gtatggattg tttagaacaa aagcggatga atcatatttg ataggagaag
     2101 gtaaagggcc aatagatgca tatttagata tggatggaat aatagaccta gcaaagagaa
     2161 aaaaagttga tgctatacat ccaggatatg gttttctagc ggagaatgca gaatttgctc
     2221 gtaaatgtga agagaatgga attactttta taggaccatc atctaaggta atgaatatga
     2281 tgggggataa gattaactct aaaaaaatcg caaaagaagt taatgttcaa actataccag
     2341 gagtagaaaa agcaataaga agtactgaag aagcaaagga agttgcaaac aaaataggat
     2401 atcctgttat gataaaagca tctaatggcg gtggcggtag aggtatgaga attgtacatc
     2461 gcgaagaaga cttagagctt gaatatgaaa ctgcttgtag tgaatcaaga aaagcttttg
     2521 gagaagatat tatatttata gaaaaatata tagctgatcc aaaacatata gaagttcaaa
     2581 tattaggtga taattatgga aatatagttc acttatatga aagagattgt tcagttcaaa
     2641 gaagacatca aaaaataata gaatatgcac cagctttttc attagatgat aaagtaagaa
     2701 aagagatatg tgaagatgca gttaagttat caaaacatgt gggttattct aatgcgggaa
     2761 cattagaatt tttagtagat gcaaatggtg ggcattattt tatagagatg aacacaagag
     2821 tacaggttga acatactgta acagagatgg ttacaggaat agatatagtt caaagtcaaa
     2881 tactaatagc ccaaggttac agcttagata gtgaagaaat taatataaaa tcacaagatg
     2941 atgtggaaat aagaggttac tctatccaat gtagaataac tacagaagac cctaagaata
     3001 aattcatgcc tgatacaggt aaaattcaag tttatagaac tggttcagga tttggtataa
     3061 gactagatgg aggaaatgga tttacagggg caaacataag tcctcattat gatagtttat
     3121 tggttaagac aatatcttgg gatagaacat tccaaggagc aataaataaa actataagat
     3181 ctataaaaga acttagagtt agaggtgtta aaactaatgt aggattccta gttaatgttt
     3241 taaataatcc tatattctca aatggtaaat gtagtactaa gtttatagat gaaaatccag
     3301 atttatttga aataacagaa agtaaagaca gaggaacaaa attacttcaa tttataggag
     3361 atgtaattgt aaatgataat gcttgtaaag aaaaaccatt atttgatgca ttacatgacc
     3421 caagaatgga taaggatggt tcaaaatcag aaggaagtaa aatacttttc gataagttag
     3481 gtaaatctgc atatatagaa aagataaaaa atgataagaa attactatta acagatacaa
     3541 caatgagaga tgcacatcaa tcattattag caacaagaat aagaacatat gacttattaa
     3601 aagctgctaa gccaacagaa aaatatcaaa aggatttatt ctcacttgaa atgtggggag
     3661 gagctactta tgatgttgca tatagatttt taaaagaatc tccttggcgt agattacaaa
     3721 agctaagaga agaaatacca agcataatgt tccaaatgtt gcttagagca tcaaatggag
     3781 ttggatataa aaactatcca gataatgtaa tagaagaatt tacaagagaa tctgctagac
     3841 aaggtataga tgtatttaga atatttgatt cattaaactg ggttgaaaat atgaaaccat
     3901 ctattaatac agctttagaa actggaaaaa ttgttgaagc aactatgtgt tatacaggtg
     3961 atatactaga taagactaaa actaaataca atctagaata ttatattaaa atggcacaag
     4021 aattagaaag cttgggagca gatataatag caataaaaga tatgtcaggt cttcttaaac
     4081 catattcagc ttacacatta gttaaagagt taaagaaaaa tgtaaaagca ccaatacatt
     4141 tacacactca tgatacaagt ggaaatggtg tagcaacttg tttaatggcg tcagaagctg
     4201 gagtagacat aattgatgct gcattagaat ctatggcagg acttacaagt cagccatcac
     4261 ttaatgctat agtagaggca cttaagaata cagagagaga tacaggaata gatttatttg
     4321 gatatgatga gttaggaaaa tattataagg atttaagaaa agtttataat aaatttgaaa
     4381 gtgatttaac taattcttgt gcagaaatat ataattttga aataccagga ggccaatata
     4441 caaacttaaa acctcaagct gatagtttag gattagttaa tagatttgat gaagttaaag
     4501 aaaaatataa agaagctaat gaagttgtag gagatataat aaaagtaact ccatcttcaa
     4561 aagttgtagg agacttagct atctttatga ctaaaaataa attagataaa gacaatataa
     4621 tagaagaagg taaaaattta tcattcccag attctgttgt ggattactgt aaaggtatga
     4681 ttggtcaacc agaaggtgga ataccaaaag atttacaaga ggtagttcta aaaggtgaag
     4741 aagctataac agtaagacca ggttcattat taccagcaga agattttgat gaaatagcta
     4801 aatatctaaa tgaaaagtat gatataaatg ctaatataag aaatgttata agttatgctt
     4861 tatatccaaa agtatatgaa gattatatca aacatctaca acattataat gatatatcta
     4921 aacttgaaag tgatgtattc ttctatggat taaataaaaa tgaagaatgt gaagtagaaa
     4981 tagaagaagg taaggtatta acaataagat tagttgaaat tggtgaagtt aaggaaaatg
     5041 gatttagaac tataggattt gagttaaatg gaatggttag agaagtagaa ataaaagata
     5101 agaatttctc tggaaaaatt aataatgttg aaaaagctga tatgaatgac cctcttcaaa
     5161 ttggagcaag cataccagga aaagttataa aaataatggt taaagaagaa gatgaagtaa
     5221 aagcaaatca accattaatc gttatagagg ctatgaagat ggaaactata atagttgcta
     5281 agactgatgg agtaattaag tcaatcaaag taaaagaaga tgatatggtt gaagataaac
     5341 aattattaat gataatgaag taatttaatt aaaataaatt aaaaagcact ctgaaatgtt
     5401 taataaaaaa tgttttagag tgtttttatt tattttaatt aaatatttgt taaaaaaaag
     5461 aggacaatta cctccagatg tagaaataaa gctagtaagg aaaatgttat ctaaaaattc
     5521 taaatatagt aaatattttc cataatattt cttaggaaat aacagcaaag ataaaataca
     5581 taaatttaaa aggtgggtgc ttatatgaag gtttatgata gtaaaatgtt gagaaatgta
     5641 gcggtattag gacatagcgg atgtggaaaa acaaatctaa tcgaaactat agcttacaca
     5701 gcaaacacta ataagatacc taaattaaca gacaaggtaa atatgactta tagcatggga
     5761 ttaataccta tagagtataa tgattataag tttaacttat tggatacccc aggatatttt
     5821 gactttagcg gagacgttgt gtcatctctt agagcaagtg atgcagcaat aatagtaata
     5881 gatgcaacag cgcctattca agttggtaca gagaaatcat tagaactaac tgagagtatt
     5941 cctaagataa tgtttataaa taaaatagat aatgaaaagg caagatacaa agatgcaata
     6001 gcaatgttaa gagaaaaata caataataag atagttccta tgataagtcc tatatacaaa
     6061 gataaaaact ttgtaaagtt acataatgtt tttgaaaaca tagatgattt agaaggtgaa
     6121 tttaaggaac aagctatgag tgttaaagaa gcattaatgg aacttatagc agaaactgat
     6181 gaccaaatat tagataaata ttttaatgga gaagaactaa ctacagaaga gatacaaaaa
     6241 ggtattatta taggtataca aagaggtgat attatacctg taatatgtgg ttcaactata
     6301 aataatatag gcacaaagga aatattagat acaatatcaa gttatttaga acctatcttt
     6361 acagaggaaa gcaaaccatt taggggatta gtatttaaaa ctatggttga cccatttgtt
     6421 ggaaagatgt catatataaa aattactgag ggtgtacttt ctaaggataa agatgttttt
     6481 aatataaata aaaatgtaaa agaaaaaata gctaatatat atacattgag aaatagtgaa
     6541 ttagtagaaa tagaaaaagc taaagctggt gatatagtag ttataactaa agttaattct
     6601 ctaaaaacag gagacactat atctgcagat aaagatgcag aagctttaga aaagatagat
     6661 tttcctaaac cacaaatata ttatgcagta actcctaaga ataaaggtga tgaagaaaaa
     6721 gtagcatctg tattaaataa actagtagaa gaggacccta cattacattg gtatagaaat
     6781 acagagacaa aacaagcact tttaggaggt caaggagaac ttcatataaa aacaattaaa
     6841 aataaaatga aagataagtt tggtgttgat gttgaattga atgaccttaa agttccttat
     6901 agagaaacta taaaaggaac agctgatgta caaggaaaac ataaaaagca atcaggtggt
     6961 catggtcaat atggagacgt aaaaataaga tttgaaagat gtgaatctga ttttgaattt
     7021 acagaagaaa tatttggtgg ttctgtacca aaacaatata taccagcagt tgaaaaaggt
     7081 ttaaaagact ctatgcaaaa aggtatatta gcaggatatc ctgttacaaa cataaaagct
     7141 acgctttatg atggttcata ccatgatgta gactcttctg aaatggcctt taaaatggca
     7201 gcaagtgcag cctttaaaaa aggtatggaa gaagcacacc caatactact agaacctata
     7261 atgaaactta agataactgt tccagaagaa tatatgggag atgtcatggg tgatataaat
     7321 aagagaagag gaaaaatatt tggaatggaa ccagatgaca aagggaaaca aataatattt
     7381 gcagaagctc cacaagcaga gacatttaaa tatgcaatag atttaagagc aatgacacaa
     7441 ggtagaggat actttgaaat ggaattagag agatatggag aggttccatc acagtttgca
     7501 gaaaaaataa ttgggttagc tactgcaaag taatgaacaa aataaataga aaaataatta
     7561 atatatatta ttaaaaatat ttttagggtt ttaactaggt ataggtttat gttag
//
LOCUS       DAMJVK010000147         7473 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.152, whole genome shotgun sequence.
ACCESSION   DAMJVK010000147 DAMJVK010000000
VERSION     DAMJVK010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7473
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.152"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(162..557)
                     /locus_tag="PC414_003505"
     CDS             complement(162..557)
                     /locus_tag="PC414_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HDF2465858.1"
                     /translation="MKALEFLNKAGVYYLATVDENNQAHVRPLGFVMEYNGKLTFCTS
                     NQKDMFKQLIFNPKVELCCIDQNLNTLRILGEAVFVTSKETQAKALEIMPSLGQMYSV
                     GDGKFEIFSIDKAKVSCCSMSGKKIDVEL"
     gene            698..1090
                     /locus_tag="PC414_003506"
     CDS             698..1090
                     /locus_tag="PC414_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2465859.1"
                     /translation="MSINTEKNITKNVEELTCPIRYALDIVGGKWKLPIICMLAVENP
                     IRYSSIKRKLDGITNTMLAQSLKDLESTGIVHRKQYNEIPPKVEYTLTSKGKSIVPIL
                     QQFANWGATNMQEKNTCGLSCKECRKIK"
     gene            complement(1190..1606)
                     /locus_tag="PC414_003507"
     CDS             complement(1190..1606)
                     /locus_tag="PC414_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HDF2465860.1"
                     /translation="MNWQDAFFQDTKPTFEQINEFINNPLWDKLNKTLVSTYNVEPKL
                     EYSKCSMQRGWNVKYKKRGKSLCTLYPQEGYFKALVIVSESNRVEVDLFINTCCDYIK
                     KIYNEVNFFNGSKWLMIQVDSLLVLNDMLELIKFRA"
     gene            complement(1621..2532)
                     /locus_tag="PC414_003508"
     CDS             complement(1621..2532)
                     /locus_tag="PC414_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2465861.1"
                     /translation="MHAWESIQITLDLIEANLSEEISINELANKANLSPFYYQRLFKR
                     LVNKTVMEYIKLRRLARASEYLAEHKNRILDVALNFGFASHETFTRSFKKAYGLTPEK
                     YRANPVKLNHFIKPELILNYAMVDEGVPLIADDIVIEVNRKTLSNPRTFVGIEIEVPI
                     CQLVGGETTGIAIVEELWMKLGSQRHNIPNQIPGGNEFAALYMGNAKEGNCMYMAGVE
                     VESGTSVEGYSTFELPAKEYLVCGFEAENFNELVNSAVFKADKFMERWMKKHNLTTTD
                     FAIEMYYPTTPEAAYLEHWIVPVPIEQ"
     gene            complement(2714..3736)
                     /gene="splB"
                     /locus_tag="PC414_003509"
     CDS             complement(2714..3736)
                     /gene="splB"
                     /locus_tag="PC414_003509"
                     /EC_number="4.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore photoproduct lyase"
                     /protein_id="HDF2465862.1"
                     /translation="MFNPKRIIFEKGTLDTDVGKKIYNKFRDNKDVEIINISSNRIKE
                     HIPGENLYEQYRSGKQTMVVGIRKSLKFQTCKPSANYQLPLVSGCMGRCEYCYLNTQL
                     GDKPFIRVFVNVDEILEQTKKYIEDRKPQVTIFEGAATSDPVPVEPYTNSLKKTIEFF
                     GREEKGRFRFVTKYSDVDSLLDAEHNNHTEIRFSINTPRIINEHEHYTASANKRIESA
                     VKLARAGYQIGFIIAPVFLYEGWKEEYKNLIISIKEALPKDFDKKIIFEVISHRYTTK
                     AKNRILEIYPDTLLPMENEDRKFKYGQFGYGKYVYDKEQLDDMKEFFLKEINGVFKNS
                     LIKYII"
     gene            complement(3963..5093)
                     /locus_tag="PC414_003510"
     CDS             complement(3963..5093)
                     /locus_tag="PC414_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HDF2465863.1"
                     /translation="MNFNYNLPVNILFGSGRINDVGKEVSKYGKKVLIVTGKNSTKKT
                     GLLDKTIDLLKDSKIEYEVFDRVEQNPLITTIYSGVEIIKSTGCDCVLGLGGGSIMDA
                     AKSIAFSYKNPGDLNDYIFGIRQGEEALPIILVPTTSGTGSEGNCFAVLTNSETKDKK
                     SIKKNSMYAKASIIDPELMVTMPKHIIASVGFDALAHNMEAYLSNGRNPLADVQAIYG
                     IELISENLIKVYNDVNDLEAWEKITLASTIGGMTIGTAGTALPHGMEHPVSGLRDVMH
                     GKGLAALTPTIIESSWDSDIERFGNISKVLGGTCAEDCSDAIRNFLKKIDLDIKLSDL
                     GIEKSDIDWLTDNCMKISVANIKRHPKYFNKEQIKEMYHKSL"
     gene            complement(5481..7418)
                     /gene="htpG"
                     /locus_tag="PC414_003511"
     CDS             complement(5481..7418)
                     /gene="htpG"
                     /locus_tag="PC414_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HDF2465864.1"
                     /translation="MEFEKGSISIHTENIFPIIKKWLYSDKDIFIRELISNGCDAVSK
                     HKRLVSLGEISENKSSDYKITVSVNKGEGTLKFIDNGIGMTEEEIKKYINQVAFSGAE
                     DFFNKYKDKMEESNDIIGHFGLGFYSAFMVSKKVQIDTLSYTEGATPVRWISEGGTEY
                     EISESDARNDRGTTITLFIDDDSKEFLDEFTVRGIINKYCSFLPVEIYLEDVERLERE
                     AKEAEEKAKKQKEDGKEEIVDAKVIEPLNDTNPLWLKSPKDCTDEEYKEFYRKVFNVF
                     DEPLFWIHLNVDYPFNLKGILYFPKLKNEFELTEGKVKLYNNQVFVADNIKEVIPEFL
                     LLLKGVIDCPDLPLNVSRSFLQNDRDVSKISKHIIKKVADKLKSLCKNEREEYNKFWD
                     DIQIFIKYGCLKDESFYEKVKECILFKTIDDEYITLQDYLEKCKDKHENKVFYVSDKE
                     QQSQYIKLFKEYDLSAVVLNSSIDTHFISFMEYKENGVKFNRIDADLSDVLKDKNENK
                     DSEENKEEIAKIEGLFKEAVGERVKNYSVEGLKNEDTPAMVLVSEQSIRMAEMQSRFA
                     GMDLGMNFEEEKTLVINENSPIIKKLVSLKDDEEKKDKITLICNQIADLALLSNKELK
                     PDELDSFVQRSNKLMSLLIEL"
BASE COUNT         2432 a         1294 c          783 g         2964 t
ORIGIN      
        1 tattcaaaat agtatatcat tattgaaata cacatctaat aactaaaaat ttttacatat
       61 tcattagtta aaaatctttt tattgcattt aaattaaatc tatcaatatt tcagtaataa
      121 cgttttctta attttcgcta tggatgtcga ttcaattata attaaagctc tacatcaatc
      181 tttttaccac tcattgaaca acaactaact tttgccttat caatagagaa tatttcaaac
      241 ttaccatctc ctacagagta catctgacct aaacttggca ttatttcaag tgccttagct
      301 tgtgtttctt ttgatgtaac aaatactgct tcacctaaaa tacgtagagt attaaggttt
      361 tggtctatac aacaaagttc aacctttgga ttaaaaataa gttgcttaaa catatccttt
      421 tgattagatg tgcaaaatgt aagtttacca ttgtattcca taacaaatcc taaaggacgc
      481 acatgtgctt gattgttctc atcaactgtt gctaaataat aaactcctgc cttgttcaaa
      541 aattctaatg ctttcataat tcttacaccc cttttaaata cttgtattaa ggattatcct
      601 ttgatataat tttaatataa aatacagaaa aaacaagtat gcagtttttt ctgataaagt
      661 ataaaaaaga tactactata actaaggagt aattaaaatg agtataaata ctgaaaaaaa
      721 tataactaaa aacgttgaag aattaacctg tccaatccgt tatgcattag acattgtcgg
      781 tggtaagtgg aaattaccta ttatatgtat gttagcagtt gaaaatccaa ttagatatag
      841 tagcataaaa agaaaattgg atggcattac aaatactatg ctagctcaat cattaaaaga
      901 tttagagtct actggtattg ttcatcgtaa acaatataat gaaattcctc caaaagttga
      961 atatacatta acaagtaaag gtaaaagtat tgtgcctatt ttacaacaat ttgctaattg
     1021 gggtgctact aatatgcaag aaaaaaacac atgtggactt agttgcaaag aatgtagaaa
     1081 gataaagtaa atttaaattt actaaattat tcatattata gactatatat taatttatct
     1141 gattgaatat aaaaacagga tgacttatat attcatcctg ttctatatgt taagctctaa
     1201 atttaattag ttcaagcata tcgtttaata ccaataaact atctacttga atcattagcc
     1261 atttacttcc attaaaaaag tttacctcat tatatatttt tttgatataa tcacaacaag
     1321 tattaataaa taaatcaacc tctactctat tgctttcact tacaataacc aaagctttaa
     1381 aataaccttc ttgtggatat aaagtacata gtgatttacc tcttttttta tattttacat
     1441 tccaacctct ttgcattgaa catttactgt attcaagctt aggttctaca ttataagtag
     1501 aaaccaaagt tttatttaac ttgtcccata atggattatt aataaattca ttaatttgct
     1561 caaatgtagg tttagtatct tgaaaaaacg catcttgcca attcatattc atcatccttt
     1621 ctattgttca ataggaaccg gaacaatcca atgttccaga tatgctgctt caggtgtagt
     1681 ggggtaatac atctcaattg caaaatcagt agttgttaaa ttatgtttct tcatccatct
     1741 ttccataaat ttatctgctt taaaaacagc tgaattaact aactcattaa agttttctgc
     1801 ctcaaatcca catactaaat attctttagc tggcaactca aaagtactat atccttctac
     1861 tgatgtacct gactctacct ctacacctgc catatacata caatttcctt cttttgcatt
     1921 tcccatataa agagcagcaa attcatttcc acctggaatt tgatttggta tattatgtct
     1981 ttgacttcca agtttcatcc ataattcctc tactattgca attccagttg tttcaccgcc
     2041 caccaactgg caaataggta cttctatttc tattccaaca aatgtccttg gattactcaa
     2101 tgtcttacga ttgacttcaa tcactatatc atctgcaatc agtggaacac cttcatcaac
     2161 catagcataa tttaaaatta attctggctt aataaaatga ttgagtttta ctggatttgc
     2221 tctatatttt tctggagtta atccatatgc ttttttaaag gaacgtgtaa atgtctcatg
     2281 actagcaaat ccaaaattta aagctacatc aagtatacga ttcttatgtt ctgctaagta
     2341 ttcagaagct cttgcaagac gacgaagctt tatatactcc attacagtct tatttaccaa
     2401 tcttttaaat agtctttgat agtaaaatgg tgataaattt gctttatttg ctaattcatt
     2461 aattgatatt tcttctgata aatttgcttc tattaaatcc aatgtaattt gaatagactc
     2521 ccatgcatgc atactataac tcctcctgat atttttgtta ctcttatcct ttatagtatc
     2581 aaaactagaa aaaatgttct tgatagatag tgcccttttt aattttctta tatgataatg
     2641 ctaaatctca ttgctttatt ataaacataa aatatataaa aataaaatat agcaaatctt
     2701 taggtaagat ttgctatatt atatatttta ttaaactatt tttaaatact ccatttattt
     2761 ccttaagaaa gaactctttc atatcatcta attgttcttt atcatacacg tacttaccat
     2821 atccaaattg accgtattta aactttctat cttcattttc cattggcaat agtgtatctg
     2881 gataaatctc tagtattcta ttttttgctt tagttgtata tctatgagat ataacttcaa
     2941 atataatttt tttatcaaaa tcctttggta aagcttcttt tatacttata atcagatttt
     3001 tatactcttc tttccaacct tcatataaaa acacaggtgc tattatgaaa ccaatctgat
     3061 aaccagccct agcaagtttt acagctgact ctattctttt atttgcagag gctgtataat
     3121 gctcatgttc atttataatt ctaggagtat tgatactaaa tcgtatttct gtatgattat
     3181 tatgttcagc atccaataag ctatccacat cactgtattt agtaacaaat ctaaatcttc
     3241 ctttttcctc tcttccaaaa aactctattg ttttcttcaa agaattagta tatggctcta
     3301 ctggaactgg gtctgatgta gctgcccctt caaatatggt tacctgtggc tttctatctt
     3361 caatatattt ttttgtctgt tcaagtattt catctacatt tacaaacacc cttataaatg
     3421 gtttatcacc taattgtgtg ttcaaataac aatactcaca tcttcccata catccactga
     3481 caagtggtag ttgataatta gcagaaggtt tacatgtttg aaacttcaga ctttttctaa
     3541 tcccaactac catagtctgc ttcccacttc tatattgttc atatagattt tcacctggaa
     3601 tatgttcttt aatcctattt gatgatatat taattatctc tacatcttta ttatctctaa
     3661 atttattgta tattttttta cccacatcag tgtctaaagt tcctttttcg aatattattc
     3721 ttttgggatt aaacatttat ttcacctcta cttaaagttt ttaaatcgaa gaattttata
     3781 aaatattact taattaatct tccctttttt aaactccttt atttattaga aagtaaaata
     3841 aaagcttaag acaaatttat tatatatata acttctatac acaatttttt tactattaat
     3901 tacttataat tttttattat taatttataa tttttatata aaactaaata tgtaaagtca
     3961 aattataaag acttatggta catttccttt atttgttcct tattaaaata ctttggatgt
     4021 ctcttaatat ttgcaactga tattttcata caattatcag ttaaccaatc tatatctgat
     4081 ttttcaatac ctaaatcact tagctttata tctaaatcta tttttttaag gaaatttcta
     4141 attgcatctg aacaatcttc tgcacaagtt cctcctaata cctttgaaat attaccaaat
     4201 ctttcaatat cactatccca tgaactttct ataatagtag gagtcaaagc tgcaagtcct
     4261 tttccatgca ttacatctct aagaccactt acaggatgct ccattccatg tggcaaggct
     4321 gtacccgctg taccaatcgt cattcctcca atagtacttg caagtgttat tttttcccat
     4381 gcttctaaat cattaacatc attatatacc tttattaggt tttctgatat aagttcaatc
     4441 ccataaatag cttgtacatc tgcaagaggg tttcttccat tagaaagata tgcttccata
     4501 ttatgggcaa gtgcatcaaa tccaactgat gcaattatat gttttggcat tgtaaccata
     4561 agttctgggt caattattga tgcttttgca tacatactat tcttctttat agatttttta
     4621 tctttagttt ctgaatttgt aagtactgca aaacaatttc cttcactacc tgtacctgat
     4681 gttgttggaa ctaagattat aggtaaagct tcttcacctt gtctaattcc aaatatataa
     4741 tcatttaaat ctcctgggtt tttataagaa aaagctatac tcttggcagc atccataatg
     4801 cttccaccac caagacctaa aacacaatca catccagttg attttataat ttctactcct
     4861 gaatatatag tagttataag aggattttgt tctactctat caaaaacttc atattctatt
     4921 ttagagtctt ttagtaaatc tattgtttta tcaagtaatc cagttttctt tgtactattt
     4981 ttacctgtta ctatcaatac ttttttgcca tatttagata cttcttttcc cacatcatta
     5041 attctgccgc tcccaaataa gatatttact ggcaaattat aattaaaatt catgctaaaa
     5101 cctcctattt ctactttagt aataatttta tgtttttaac aaagtgtaat tatgtattta
     5161 aacataaact aaaaaacaat cttttactta attatatgta ttatcttatt atatatcaag
     5221 ttaactatat ttaactaaat agagtttttt attagaacct acttaaatat ttaaaaatta
     5281 accaaagata aaaaaataaa gaacggtctg tctcaaaata gagataaata tatttttcat
     5341 tgttgaaatg attaaaataa atttaatttc acataaaaaa agcgcctcat gaactgaaaa
     5401 gttcattttg agacacttct ttcttatcac tgatattgaa tactttttat aaatttgctt
     5461 caatgtattc aaattcaatc ttataattct atcaaaagac tcataagctt attacttctt
     5521 tgtacaaatg aatctaactc atctggcttt aattctttat tagaaagtaa agctaaatct
     5581 gctatctggt tacaaataag cgtaatctta tctttctttt cctcatcatc tttaagagaa
     5641 accaattttt tgattattgg actgttctca tttattacaa gagttttttc ttcttcaaaa
     5701 ttcattccta aatccattcc agcaaatctt gactgcattt ctgccattct aatagattgt
     5761 tctgatacta acaccatagc tggagtgtct tcatttttca atccttctac agaataattc
     5821 ttcactcttt ctccaacagc ttctttaaac agaccttcta tcttagctat ctcttctttg
     5881 ttttcttcac tatctttatt ttcattttta tcttttaata catcagaaag gtctgcatct
     5941 attctattaa acttaactcc attctcttta tattccataa atgaaataaa atgagtatct
     6001 attgatgagt ttaaaacaac agcacttaag tcgtattctt taaatagttt aatgtattga
     6061 gattgctgtt ctttatcact tacataaaat accttatttt catgtttgtc cttacacttt
     6121 tctaaataat cttgtaatgt tatatactca tcatcaatag ttttgaataa tatacattct
     6181 tttaccttct catagaaact ttcatctttt aagcaaccat atttaataaa tatttgtata
     6241 tcatcccaaa acttattgta ttcttctctt tcatttttac aaagagattt taacttatca
     6301 gctacttttt taattatatg tttagaaatt ttgcttacat ccctgtcatt ttgtaagaaa
     6361 cttcttgata catttaatgg taagtctggg cagtctataa ctccttttaa taaaagtaaa
     6421 aattctggta taacttcttt tatattgtca gccacaaaca cttgattgtt gtataatttt
     6481 acttttcctt cagtcagttc aaactcattt tttaatttag ggaagtaaag tatacccttt
     6541 aaattgaaag gataatctac atttaaatgt atccaaaata aaggttcatc aaatacattg
     6601 aatacctttc tataaaactc tttatactcc tcatcagtac agtcttttgg agattttaac
     6661 catagaggat tagtgtcgtt taaaggctca attacttttg catctacaat ctcttctttt
     6721 ccatcttcct tttgtttttt agctttttct tctgcttctt tagcttctct ttctaatctt
     6781 tcaacatctt ctaaatatat ttctactggt aagaaagaac agtatttatt tattattcct
     6841 cttacagtaa attcatctaa aaattcttta ctatcatcat caataaatag tgttatagta
     6901 gttcctctat catttctagc atctgattca gatatttcgt attctgtacc accttcagat
     6961 atccatctta caggagtcgc tccttctgta tatgaaagtg tatctatttg taccttttta
     7021 gacaccataa atgcagaata aaatcctaat ccaaaatgac ctattatatc atttgactct
     7081 tccattttat ccttatattt attaaagaaa tcttcagcac ctgaaaaagc aacttgattt
     7141 atgtattttt ttatctcttc ttcagtcata ccaattccat tgtcaataaa tttaagtgta
     7201 ccttctcctt tatttacaga tactgtaatt ttataatctg atgacttatt ttctgatatt
     7261 tctcctagtg atacaagtct tttatgttta cttactgcat cacatccatt acttataagc
     7321 tctcttataa atatgtcttt atcagagtat aaccattttt taattatagg aaaaatattt
     7381 tctgtatgaa ttgaaatact ccctttttca aattccattt tttatccctc ctcattttat
     7441 aattaatatt ttaaattttt taaaatggta tgt
//
LOCUS       DAMJVK010000148         7467 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.153, whole genome shotgun sequence.
ACCESSION   DAMJVK010000148 DAMJVK010000000
VERSION     DAMJVK010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7467
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.153"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            98..1075
                     /locus_tag="PC414_003512"
     CDS             98..1075
                     /locus_tag="PC414_003512"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HDF2465865.1"
                     /translation="MRDKIIEIILNNKSEFISGEELSKQLGVSRAAIWKHMKALKEEG
                     YNIESVNKKGYRLAENPTDLLSPQNIYYKLDTEFIGKNIEHLDTIDSTNEHAKRIAIN
                     SVDGTVILSEHQDMGKGRLGRRWDSKPHEGIWMSIILKPDMEPFKAPFITLIAGASVT
                     KALNNLGANTLIKWPNDVILNGKKICGILTELSAEIERTNYVVLGIGINVKIMDFEQE
                     IADKATSLYKEGYMLSRVDIVRNVLTEFEKLYLDYTVNNSKEKTLKICRDYSAIIDKN
                     IYVLKGDNRELVKCLDINEEGNLLVQRSDGSIKEIISGEVSIRGEKGYV"
     gene            1068..2036
                     /locus_tag="PC414_003513"
     CDS             1068..2036
                     /locus_tag="PC414_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HDF2465866.1"
                     /translation="MYNNILDIEGLKVGQVEDRNGLTGCTVVICEEGATCGVDVRGSA
                     PGTRETDLLDPVNMVQKVHAVVLAGGSAYGLESTCGVSRYLEEKGIGFDVGVGKVPIV
                     VGAVLFDLGVGSFKCRPDLKMGYRACEVANTDILNQGNYGAGCGATVGKIRGQEYAMK
                     GGIGSYSIKLDNGLIVSALIAVNALGDVYENGKVIAGALNDKKTETIDTYKLMKQGES
                     KGGFSVDNTTIGIIATNAKLTKAGCKKISQMGHDGYAKSIFPIHTPHDGDTIFTISTG
                     EIEADITLVGSLAVEVVEKSIINAVKKADSVENIMAYKDIVKKREI"
     gene            2219..2809
                     /locus_tag="PC414_003514"
     CDS             2219..2809
                     /locus_tag="PC414_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDF2465867.1"
                     /translation="MMNTSASVRKVNVRKMTIIGVLSAISIMLSMTPLGFIPVGPTKA
                     TIMHIPVIIGAIMEGPIVGAAIGFIFGISSLLNAITNPTATSFVFINPLVSILPRVMI
                     GVLAYYVYQLIIKATNKVYISGLITGAIGSLLNTAGVLGMIYVLYADKYLHAMGKSGS
                     AGKVIMALAAANGVPEAIVGALVVAAVATVLKKSKK"
     gene            2828..3598
                     /locus_tag="PC414_003515"
     CDS             2828..3598
                     /locus_tag="PC414_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HDF2465868.1"
                     /translation="MLLVFDVGNTNMVLGIYKGDKLVNYWRIKTDREKTSDEYGILIS
                     NLFDYDNVNISDIDDVIISSVVPNVMHSLENFCIKYCKKQPLIVGPGIKTGLNIKYDN
                     PKQVGADRIVNAVAGIEKYGAPSILVDFGTATTFCAISEKGEYLGGTIAPGIKISSEA
                     LFQSASKLPRVELAKPGMTICKSTVSAMQSGIIYGYVGLVDKIISMMKKELNCDDVKV
                     IATGGLAKLIASETKSIDYVDGFLTLEGLRIIYEKNQE"
     gene            3735..4703
                     /gene="dusB"
                     /locus_tag="PC414_003516"
     CDS             3735..4703
                     /gene="dusB"
                     /locus_tag="PC414_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HDF2465869.1"
                     /translation="MKIGNLELKNKVFLSPMAGVTDLPFRLICKEQGCGLLYTEMING
                     KALCYDDENTKKMLKIEEEEHPVAVQIFGSEPEFMGRATEIMNDYSNEILDINMGCPA
                     PKVVKNGDGSALMKNPKLAEEVLRAVVKNSKKPVTLKIRKGWDDNSVNAVEIAKIAED
                     CGISALAIHGRTREQFYTGKADWDIIAEIKKNLSIPVIGNGDVFTIEDSINMLDKTGC
                     DAIMIGRGAQGNPWIFKRINHYMNTGEILPEPTLNEKISTAIKHLKLAVEEHGEYVAV
                     REMRKHIAWYLKGLRNSAKLRDEINKIEDYQEVVSKLEYYMEDSLT"
     gene            4812..5291
                     /gene="greA"
                     /locus_tag="PC414_003517"
     CDS             4812..5291
                     /gene="greA"
                     /locus_tag="PC414_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HDF2465870.1"
                     /translation="MEENKEFLLTQEGYDKLEEELENLKVVKRKEVAERIKVAISFGD
                     LSENAEYDEAKKEQAQVEERILKLENMVRKAVIIDESKIDLNVVTIGSIVKVKDLEFD
                     EEVEYTIVGSTEADPYDGKISNESPVGKALLGRAAKEVVEVQVPDGVAKFEILEIRR"
     gene            5306..6835
                     /gene="lysS"
                     /locus_tag="PC414_003518"
     CDS             5306..6835
                     /gene="lysS"
                     /locus_tag="PC414_003518"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDF2465871.1"
                     /translation="MKNNQQSNEEAQIQEDLSEVLQVRRDKLKKLQESGRDPFKESRY
                     DRTHYSMDIKDNFDSLEGKTTKIAGRIMSKRIQGKAGFIDIQDQEGRIQSYVRLDAIG
                     EEEYSVFSTYDIGDIVGIEGEIFKTKKGEISVKAKSVVLLCKSLQVLPEKYHGLKDQE
                     LRYRQRYVDLIVNPEVKNAFLIRTKALKALRAYLDDRGFLEVETPILNTIAGGANARP
                     FITNHNTLHIPMYLRIANELYLKRLIVGGFDKVYEMGRMFRNEGMDLKHNPEYTAIEL
                     YQAYADYTDMMEITENVIAHMAEVATGSMIVNYQGTEINFTPPWKRMSMEDCVKEYSG
                     VDFSTINTDEEALEVAREKGIEIKPGMRRGEVINAFFEEFGEDKLIQPTFITHHPVEV
                     SPLSKRNVEDPRRTDRFEAFANKWELANAFSELNDPIDQKGRFIDQLRKRELGDDEAF
                     EMDEDFLKALEVGLPPTGGLGIGIDRVIMLLTNSPSIRDVLLFPTMKPIDNNPNKEEE
                     N"
     gene            7144..7338
                     /locus_tag="PC414_003519"
     CDS             7144..7338
                     /locus_tag="PC414_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465872.1"
                     /translation="MFQVINFLCLFCVGFLVLDVMIYFCKDAMDIFEGTTNVNKVKSI
                     NVWADRVRANSYSYENKIAK"
BASE COUNT         2980 a          755 c         1508 g         2224 t
ORIGIN      
        1 aaatttaaaa ttaatagtat taaaagtata ttaataatgt ataattattt attagaagaa
       61 aataagtcat gaaattatgt catattagag gtgaaatatg agagataaaa taatagagat
      121 tatattaaat aataaatctg aatttatttc aggagaagaa ttatcaaaac aattaggggt
      181 gtcacgtgca gctatatgga aacatatgaa agccttaaaa gaagagggat ataatataga
      241 atctgtaaat aaaaaagggt atcgattagc ggaaaatcca actgatttat taagcccaca
      301 gaatatttac tataaattag atacagagtt tattggaaaa aatattgaac atcttgacac
      361 tatagattca acaaatgaac atgctaaaag gatagctata aatagtgtag atgggactgt
      421 gattttaagt gaacatcaag atatggggaa aggacgattg ggaagaagat gggattcaaa
      481 acctcatgaa ggaatctgga tgagtattat attaaaacca gatatggaac cattcaaagc
      541 cccatttata actttaatag caggagcaag tgtaacgaaa gctttaaaca atctaggagc
      601 aaatacatta ataaaatggc caaatgatgt tattttaaat ggaaaaaaaa tatgtggtat
      661 attaacagag ttatctgctg aaattgaaag aacaaattat gttgttcttg gaattggaat
      721 aaatgttaaa ataatggatt ttgaacaaga aatagctgac aaagccacat cattgtataa
      781 agaaggatat atgttatcca gagtagatat tgttaggaat gtattaactg aatttgaaaa
      841 attatattta gattatactg taaataattc caaagaaaaa accttaaaaa tatgtagaga
      901 ttattctgca attatagata aaaatatata tgtattaaaa ggagataata gagaactagt
      961 gaaatgttta gacataaatg aagagggaaa tttattagtt caaagaagtg atggaagtat
     1021 aaaagaaatt atttcaggtg aagtttctat aagaggagaa aaaggatatg tataataata
     1081 ttttggatat agaaggctta aaggttgggc aggttgagga tagaaatggt cttactgggt
     1141 gcacagtagt aatatgtgaa gaaggagcta cttgcggtgt tgatgtaagg ggctctgctc
     1201 caggtactag agaaaccgat ttattagacc ctgtaaatat ggttcaaaaa gttcatgcag
     1261 tagttttggc tggaggttct gcatatggtc ttgaatccac ttgtggtgta agtagatact
     1321 tagaagaaaa aggaattggc tttgatgtag gtgtaggtaa agtgcctata gttgtaggag
     1381 cagttttatt tgatttagga gtagggagct ttaaatgtag accagattta aagatgggtt
     1441 atagagcttg tgaagttgca aatactgata ttttaaatca aggaaattat ggagcaggtt
     1501 gtggagccac tgttggaaag ataagaggtc aggaatatgc tatgaaaggc ggaataggta
     1561 gctattctat aaaattagat aatggtctaa ttgtatctgc attaattgct gtaaatgctc
     1621 ttggagatgt ttatgaaaat ggtaaggtta tagctggtgc tttaaatgat aaaaagacag
     1681 aaacaattga tacatataaa ttaatgaaac aaggtgaaag taaaggtgga tttagtgtag
     1741 ataatactac aattgggata attgcaacaa atgctaagtt aaccaaagct ggatgtaaaa
     1801 agatatctca aatgggacat gatggatatg ctaagtctat ttttcctatc catacacctc
     1861 atgatggaga tacaatattc acaatatcaa caggagaaat agaagcagat ataacattag
     1921 taggttcatt ggctgtagag gttgtggaaa aaagtataat aaatgcagta aaaaaagcgg
     1981 atagtgtaga aaatataatg gcatataaag atatagtaaa aaaacgagaa atatgatgaa
     2041 attagcatat ttttcttgtc aaattgttga caatggcaac tcatcttgat aaaattattc
     2101 ttgttagagc gggcatccta caatggtagc ttgactcata gttataagaa aaaacgaaaa
     2161 aaattaaaaa attaagtagt ccggcatctt aatgagctgg aacggaggtg tattttttat
     2221 gatgaacaca agtgcatcag taagaaaagt aaatgtaaga aagatgacta taataggagt
     2281 actatcagct atttctatta tgctatctat gactccattg ggatttattc cagttggacc
     2341 tacaaaagca actataatgc acataccagt tattattgga gctattatgg aaggaccaat
     2401 agttggggca gcaataggat ttatatttgg gatatcaagt ctcttaaatg ctataacaaa
     2461 cccaacagca acatcttttg tttttataaa tccattagtt tcaatattac caagagttat
     2521 gataggtgta ttagcttatt atgtatatca gttaataatt aaggctacaa ataaggtata
     2581 catatcaggt ttaattacag gggcaatagg ttctctgtta aatacagctg gagttttagg
     2641 aatgatatat gtcttatatg ctgacaagta tcttcatgca atgggtaaaa gtggttcagc
     2701 aggaaaagta ataatggctc ttgccgcagc aaatggggtg ccagaagcaa tagtaggggc
     2761 tttagtagta gctgctgttg ccactgtact taagaaaagc aaaaaataga aagaaggtta
     2821 atgacatatg cttctagtat ttgatgttgg aaatactaat atggttttag gtatatataa
     2881 aggtgacaaa ttagttaatt actggagaat taaaacagat agggaaaaaa cgtctgatga
     2941 atatggaatc ctgataagta acctatttga ttatgataat gtgaatataa gtgatattga
     3001 tgatgttata atatcatctg tagttccgaa tgttatgcat tctcttgaaa acttttgtat
     3061 aaagtactgt aaaaaacagc cattaatagt aggtccaggc ataaaaacag gtctaaatat
     3121 aaaatatgat aatccaaaac aagttggggc agatagaata gttaatgctg tagcagggat
     3181 agaaaagtat ggagcaccaa gtatacttgt tgattttgga acagcaacta cattttgtgc
     3241 tatctctgaa aaaggtgaat atttgggtgg aacaatagca ccaggaataa aaatatctag
     3301 tgaggcgtta tttcaaagtg cgtctaaatt acctagagta gaattagcta agccaggtat
     3361 gactatttgt aagagtactg tatcagccat gcaatctgga ataatttatg gatatgttgg
     3421 tttagttgac aaaataataa gtatgatgaa gaaagaattg aattgtgatg atgttaaggt
     3481 tatagctaca ggtggattag ctaaactgat tgcttcagag acgaaaagta tagattatgt
     3541 agatggtttt ttaacactag aaggattgag aataatatat gaaaaaaacc aagaataatt
     3601 aatatacagg gaattgggtt atatatccaa ttcccttttt atgatagatt tctagctata
     3661 tgtggtatat tataatgagg aaataagaat ttaaagttaa atcacaaaat aaaggcaaaa
     3721 ataggagaaa aacaatgaaa ataggaaatt tagaattgaa aaataaagtt tttctatcac
     3781 ctatggcagg tgtaacagac cttccattta gattaatttg taaagaacaa ggatgtgggc
     3841 ttttatatac agaaatgata aatggtaagg cactttgcta tgatgatgaa aatactaaaa
     3901 aaatgcttaa gatagaagag gaagaacatc ctgtagcagt tcagatattc ggttcagaac
     3961 cagagtttat gggaagagca acagagataa tgaatgatta ttcaaatgag attctcgata
     4021 taaatatggg gtgtccagct ccaaaagtag ttaaaaatgg agatggttca gcacttatga
     4081 aaaatcctaa gcttgcagaa gaggtattga gggcagtagt gaagaactca aaaaagccag
     4141 tgactttaaa gattcgaaaa ggctgggatg ataatagtgt aaatgctgtt gaaatagcaa
     4201 agatagcaga ggattgtgga ataagtgctt tagcaataca tggaagaact agagaacagt
     4261 tttatacagg caaagcagac tgggatataa ttgcagagat taagaaaaat ttgagtatac
     4321 ctgttatagg aaatggtgat gtatttacaa tagaagattc aataaatatg ttagacaaaa
     4381 ctggttgtga tgctataatg ataggtaggg gtgctcaagg taacccttgg atatttaaaa
     4441 gaataaacca ttatatgaat acaggcgaga ttttgccaga gccaactctt aacgaaaaaa
     4501 taagtactgc cattaagcat ttaaaacttg cagtagaaga acatggagag tatgtagctg
     4561 taagggaaat gagaaaacac atcgcttggt atttaaaagg acttagaaac tctgcaaaat
     4621 taagggatga aattaacaaa atagaagact atcaagaagt tgtgtcgaaa ttggagtact
     4681 atatggaaga ctccttgaca taaaaataat agtgacttat aatatagcgc ataaattaaa
     4741 aaaagattat aaaattttta aaatataatt aggaaaattc ctaagaaatt tgtgaaggag
     4801 ttgtatttat aatggaagaa aataaagagt ttttattgac acaagaggga tatgataaat
     4861 tagaagaaga attagaaaac ttaaaggttg taaagagaaa agaagttgca gagagaataa
     4921 aagttgctat atcttttggt gatttatctg aaaatgctga gtatgatgaa gctaaaaagg
     4981 aacaagcaca agttgaagaa agaattttaa aattagaaaa tatggttaga aaagctgtta
     5041 tcatagatga aagcaaaata gaccttaatg ttgttacaat aggatcaata gttaaagtta
     5101 aggatttaga atttgatgaa gaggttgaat atactatagt tggttcgaca gaagcagacc
     5161 catatgatgg taaaatatca aacgaatctc ctgtagggaa agcactttta ggtagagctg
     5221 cgaaagaagt tgtggaagtg caagttcctg atggagttgc taagtttgaa atattagaaa
     5281 taagaagata atttggagga aaaaaatgaa aaataatcaa caaagcaatg aagaagcgca
     5341 aattcaagaa gaccttagtg aagtacttca agtaagaaga gataaactaa aaaaattaca
     5401 agagtcaggt agagatcctt ttaaagaaag tagatatgat agaactcatt attctatgga
     5461 tataaaagat aactttgatt ctcttgaggg taaaactaca aaaatagcag gacgtataat
     5521 gagcaagaga atacaaggga aagctggctt tatagatatt caagaccaag agggaagaat
     5581 ccaatcttat gtaagactag atgctatagg agaggaagaa tatagtgtat tttcaacata
     5641 tgatattgga gatatagttg gaattgaagg agaaatcttt aaaactaaaa aaggtgaaat
     5701 atctgtaaag gctaaatctg tagtattact atgtaaatct ttacaagttt taccagaaaa
     5761 atatcatgga ttaaaagacc aagaactaag atatagacaa agatatgttg acttaatagt
     5821 aaatccagaa gtaaaaaatg cttttttaat tagaacaaaa gcattaaaag cattaagagc
     5881 atacttagat gatagaggat ttttagaagt tgagactcca atattaaata ctatagcagg
     5941 tggggcaaat gctagaccat ttataactaa tcataatact ttacatatac caatgtattt
     6001 aagaatagcg aatgaattgt atttaaaaag acttattgtt ggtggatttg ataaagttta
     6061 tgaaatggga agaatgttta gaaatgaagg tatggattta aaacataatc ctgaatatac
     6121 tgcaatagaa ttatatcaag catatgcaga ctatactgat atgatggaaa taacagaaaa
     6181 tgttattgca catatggctg aagttgctac aggaagtatg atagttaact atcaaggaac
     6241 agaaataaac tttacaccac cttggaaaag aatgagtatg gaagattgtg ttaaagaata
     6301 ttctggtgta gatttttcta ctataaatac agatgaagag gcactagaag ttgctagaga
     6361 aaaaggtata gagataaaac ctggaatgag aagaggagaa gtaataaatg ctttctttga
     6421 agaatttgga gaagataaat taatccaacc aacatttata actcaccatc cagttgaagt
     6481 atctccatta tctaagagaa atgttgaaga cccaagaaga actgatagat ttgaggcatt
     6541 tgctaataag tgggagttag ctaatgcatt ttctgaactt aatgacccta ttgaccaaaa
     6601 aggaagattt atagaccaat tgagaaaaag agaattaggt gatgatgaag cttttgaaat
     6661 ggatgaagat ttcttaaaag cattagaggt aggtctacct cctacaggag gactaggaat
     6721 aggtatagat agagtaatta tgttattaac taactctcca tctataagag atgtattatt
     6781 attcccgact atgaaaccta ttgataacaa tccaaataaa gaagaagaaa actaagaaat
     6841 attagcattc tactaagttt taaataaagt ttagctatta aattatcaat aaatagctaa
     6901 attttattta aaatgtcagt gtggaatgct ttttatatgc ttcaaagatt cttttagata
     6961 tttaatttat aaattaccaa caaacgttag aaattgatat tagtttttgt tgattgagac
     7021 tttatattta gaaaatatga ttgaaagtaa tttattatga aaatatttag aataatttat
     7081 gagaaggtaa aaagaataat ttatgagaat actatatata aaaaattatt gggggaatgt
     7141 ctgatgtttc aagtaattaa ttttttatgt ttattttgtg ttggattttt ggttcttgat
     7201 gttatgatat atttttgtaa ggatgcaatg gatatttttg agggtacaac taatgttaat
     7261 aaagttaaga gtataaatgt ttgggcagat agagtgagag ctaattcata ttcttatgaa
     7321 aataaaattg caaaataggt aaaattatat atgcatgtat tgtttgtaat atcaagggtt
     7381 atatctatta gttttaatta tatttattaa aaaaaccaaa agttataacc ttttttaaaa
     7441 taaaaaacaa aaaaagtatt gacgaaa
//
LOCUS       DAMJVK010000149         7373 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.154, whole genome shotgun sequence.
ACCESSION   DAMJVK010000149 DAMJVK010000000
VERSION     DAMJVK010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7373
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.154"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            176..3355
                     /locus_tag="PC414_003520"
     CDS             176..3355
                     /locus_tag="PC414_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF2465873.1"
                     /translation="MKIDMIYKLIRRNTEPSRWSRGVKYYTNQLVDYIDIDIDDLQNN
                     LLTFRGGVNSEDNYSFYFNSITIDIKHFKIIENDCSCADCQSKGKSIFICKHIVAIVI
                     KSLKQIKNKKDVPPILIDILGADKDKLSNTNSKTSLDLSKQLLSLLDNTKRERVNLDV
                     LINVDSNKAIAEFKIGINKMYILKNITEFAYSKLNGYELDFGKKFTYNPNTHYFADED
                     EKLVDIIEEYGTNLSYNFSSSHSKKFMEINASSLKQFLNAIKYKDFTLIFSKEEYHPK
                     ILNDTLPVRFDFKNIEDKIVLYSKDSLPVPLTKKNDVLLYDGNIYLLSHKKAHDYSKI
                     YQILSKTKELKFDKEDSKDVLGLLVPRLKSISQEVHLDDNIKNNITKDFKSEFYFDMI
                     DGNICCDVKYIYDDEDKKFVLPNIQKEATIENKLTSSQFTKENNHYVFKGTDHDLFTF
                     LDLQLESLKEFGDIYYSEKFKLKKIYNASSIQASINQTNQNYLEFNFNISDINPKEYK
                     DILKAFQEKRTFYKLKDGNFIDLSERETKDFFELVENLDIMDKSKNNKIHKNKALYIN
                     DVIRSKNLKFIDGKKELDSICEKFRNFDSINLEIPANLNANLRDYQINGLNWFKVLDY
                     YKFGGILADEMGLGKTIQTIAFLLSLSNKKSLIVTPTSLIYNWKNEFEKFAPDIKVLL
                     IHGNKRDREKCFMELENFDVILTTYGTLRNDLDKYSEIKFDYCILDEAQNIKNPVALV
                     TESVKSINAENKFALTGTPMENNLLELWSIFDFIMPGYLYSKAKFQELFINKEDNVKN
                     LKKLIKPFILRRSKKQVMKELPDKIEKNFFVELNKEQKKIYSVYSKDIQDKMKDKNLK
                     KDKIVIFSYLTKLRQLCLDPSIVVKDYNKKSSKIETCLEILRDSINENHKILLFSQFT
                     SVLKNISKELDKYKIKYHYIDGKTNAKERLELVDEFNNSMDKKVFLISLKAGGTGLNL
                     TSADMVIHFDPWWNPSVENQASDRAHRFGQKNSVQVIKLIAKGTIEEKIIKLQESKKE
                     LINQFINGELSNEGVLKSLSDEEIINLFN"
     gene            complement(3536..3619)
                     /locus_tag="PC414_003521"
                     /pseudo
     CDS             complement(3536..3619)
                     /locus_tag="PC414_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009005413.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
     gene            complement(3640..4404)
                     /locus_tag="PC414_003522"
     CDS             complement(3640..4404)
                     /locus_tag="PC414_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2465874.1"
                     /translation="MAYFNYNDKQCYYNEIGDGTPLLFLHGNTASSKMFNDIINFYKD
                     EYKVILIDFVGHGKSQMVDKFSADLWFDEAMQVICFLEAMNYKKVNIIGSSGGALVAL
                     NVALERPDLVNKVIADSFEGEVPLESFVQNVKIEREASKQDDGAKAFYIYNQGENWEK
                     VVDNDTEAIFEHYKTIGKFFHKPLETMQPEVLLTGSREDEFVSLISSDFFENTFSLLL
                     EKIKNGKMYLFDKGGHPAILSNGLDFSNVAKKFLEE"
     gene            complement(4560..4793)
                     /locus_tag="PC414_003523"
     CDS             complement(4560..4793)
                     /locus_tag="PC414_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465875.1"
                     /translation="MKAEDMKRKVSPIDLERILVTGGVIKHEDSIWLLDFWGNKDIAG
                     ILLMPPTRHVILHLNDCCKWKEYFRTKKKFYRS"
     gene            complement(4867..6360)
                     /locus_tag="PC414_003524"
     CDS             complement(4867..6360)
                     /locus_tag="PC414_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF2465876.1"
                     /translation="MKYTFEKFKLNEKILKSLKSLGYNIPSRVQREVIPKLLKGQNLV
                     VRSKTGSGKTASFAIPLCENINVDYNNIQALIVVPTRELALQVKDEISDIGRLKKVRC
                     SAIFGKQSIKDQIAELKQRVHIVVATPGRILDHINRGSIKLENVKYLVIDEADKMFNK
                     GFVEQMEKILLNLPKEKIVSLFSATIDEEIKYICEKYMLDYSVINIEENESDTNQKTR
                     QIDDKIIKANGREKYILLKELIYSENPKSVIIFCNTKEKVSKLYNKMSKEGFLIRELH
                     ADLSQERRIFVIKDFKNQKFNILVSSDVASRGIHIDDISLVINYDVPQDKENYIHRIG
                     RTGRKGNSGKAITIVTEKDEKYIENIETYIGYKINELKDIEKSRITHGKVLFEEYSKG
                     ILKKVSKRKKVDKNSYKSKDIKLNIESEVVKLYLNAGKKKKIRVLDIVGAFSNIKGIT
                     NDDIGVIEVQDLCSYVDILNYKGDLILKKYKEIPIKKKMVKVKRDIR"
     gene            complement(6688..7230)
                     /locus_tag="PC414_003525"
     CDS             complement(6688..7230)
                     /locus_tag="PC414_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2465877.1"
                     /translation="MKICITVGHSILKGGKSTGVNGIVDEYNYNKKLAPMLAEMLISQ
                     GNTVDVIICPERHFMSEREEFFYRVPKVNSGKYDLLVELHLNKADGTQCGTEVLYYGT
                     EGLEYAKRVSNRLGELFENRGAKKRENLYILRNTNPVAIQIESFFCDNVNDCNKANES
                     GYDYIARLITEGILNKDINM"
BASE COUNT         2697 a         1067 c          822 g         2787 t
ORIGIN      
        1 ttttcattca tagtttataa gataattcaa taaaaatcta atagtaaatt gttatttaca
       61 catatatcgt gatagataaa agctattcat atctgcctta attttagtct taaatttaga
      121 gtgtaaattg aatgcattta gattataata tatacagtga ggtaaggtga ataatatgaa
      181 aatagatatg atatataaac taataagaag aaatacagaa ccatcaagat ggtcaagagg
      241 tgtaaaatat tatactaatc aattagttga ttatatagac atagatattg atgacctaca
      301 aaataatctg ttgacatttc gtggtggagt caattctgaa gataattata gcttttattt
      361 taattcaata actatagata taaagcactt taagataatt gaaaatgatt gtagttgcgc
      421 tgactgtcaa tcaaagggca aaagtatttt tatatgtaag catatagttg ctattgtgat
      481 aaaaagctta aaacaaataa aaaataaaaa agatgttcct cctattctaa tagatatatt
      541 aggtgcagat aaggataagc tttctaatac aaattctaaa accagcttag atttaagcaa
      601 gcaattactt tctttgttag acaatactaa aagagaaagg gttaatttag atgtattgat
      661 aaatgtggat tcaaataaag caattgctga atttaaaata ggaattaata aaatgtacat
      721 tttaaaaaac attactgaat ttgcatattc aaaacttaat ggatatgaat tagattttgg
      781 gaagaaattt acttacaacc ccaacactca ttattttgct gatgaagatg agaagttagt
      841 agatattata gaagaatatg ggacaaatct ttcatacaac ttttcttcat cacattcaaa
      901 aaaatttatg gagataaatg caagctcttt aaaacaattt ttaaatgcta ttaagtacaa
      961 ggattttacc cttatttttt ctaaagagga atatcatcca aagatattaa atgatactct
     1021 acctgtaagg tttgatttta aaaatataga agataaaata gtcttatatt ctaaagattc
     1081 actacctgtt ccacttacta aaaaaaatga tgtactttta tatgatggca atatctattt
     1141 attatcacat aaaaaagctc atgattattc aaaaatatat caaatattat caaagactaa
     1201 agagttaaaa tttgataaag aggattctaa ggatgtttta ggattgctag ttcctagatt
     1261 aaaatctata tctcaagaag ttcatttaga tgataacata aaaaataata taacaaaaga
     1321 ttttaaatct gagttctact ttgatatgat tgatggcaac atatgttgtg atgtaaagta
     1381 tatatatgat gatgaagata aaaaatttgt gctccccaac atacaaaagg aagctactat
     1441 tgaaaataaa cttacttctt cacaatttac aaaggaaaat aatcattatg tgtttaaagg
     1501 aactgaccat gacctattta catttttaga tttacaatta gaatctctaa aggaatttgg
     1561 agatatatat tattctgaga agtttaaatt gaaaaaaata tataacgctt catcaattca
     1621 agcttcaatc aatcagacta accagaatta cctagaattt aattttaata tatctgatat
     1681 aaatccaaaa gagtacaagg atattttgaa agcttttcaa gaaaaacgta ctttttataa
     1741 acttaaagat ggaaacttta tagatttatc agaaagagaa acaaaagatt tttttgaatt
     1801 agttgaaaat ctagatataa tggataaatc aaaaaataat aaaattcata aaaataaagc
     1861 tctatatata aatgatgtaa tacgtagtaa aaatttaaag tttatagatg gtaaaaaaga
     1921 actagacagt atatgtgaaa aatttagaaa ttttgatagc ataaatttag aaattcccgc
     1981 aaatttaaat gcaaaccttc gtgactatca aataaatggc ctcaattggt ttaaagtttt
     2041 agattattat aaatttggtg gtatattagc tgatgaaatg ggtcttggta aaacaataca
     2101 gactattgca tttttattat cattatctaa taaaaaaagt cttattgtta caccgacatc
     2161 actcatttat aattggaaaa atgaatttga aaagtttgct ccagatataa aagttttatt
     2221 gatacatgga aataaaagag atagagaaaa atgttttatg gaattagaaa attttgatgt
     2281 aatacttaca acatatggta ctcttagaaa tgatttagac aaatatagtg aaataaagtt
     2341 tgattattgt atattagacg aagctcaaaa tataaaaaat ccagtggctt tagtaactga
     2401 gtctgtaaaa agtataaatg cagaaaataa atttgcactc actggtacac ctatggaaaa
     2461 taatctacta gaattatggt ctatatttga ttttataatg cctggctatc tatatagtaa
     2521 agcaaagttt caggaattgt ttataaataa agaagataat gttaaaaacc tgaaaaaatt
     2581 gataaaacct tttattctta gaagaagtaa aaaacaagtt atgaaagaac ttccagataa
     2641 gattgaaaaa aacttttttg ttgaattgaa taaagagcag aaaaagatat atagtgtata
     2701 ttcaaaagat atacaagata aaatgaaaga taaaaatcta aagaaagata aaatagttat
     2761 attctcttat ttaactaaac taagacaact ttgtttagac ccaagtatag tagtaaaaga
     2821 ttacaataaa aaaagttcaa aaattgaaac atgtttagaa atacttaggg atagtataaa
     2881 cgaaaaccac aaaatattat tgttttcaca atttacttct gtgcttaaga acataagtaa
     2941 ggaacttgat aagtataaaa ttaaatacca ttatatagat ggtaaaacta atgccaaaga
     3001 aagacttgaa cttgtagatg agttcaataa tagtatggat aaaaaagttt ttttaatatc
     3061 tttaaaagca ggcggaactg gtttaaatct tacatctgct gacatggtaa ttcactttga
     3121 cccatggtgg aatccatcag tagaaaatca agctagtgat agagctcata gatttggtca
     3181 aaaaaattca gtacaagtta taaaattgat tgctaaaggg actatagaag aaaaaataat
     3241 aaaattacaa gaaagtaaaa aagagcttat aaatcaattt ataaatggag aactttctaa
     3301 tgaaggtgta ttaaaaagtc tatctgatga ggagataata aacttattta actaaattat
     3361 ttaatcctac agcttaacta tatgaatttg ttctaacatt tatatagtta agctaaaata
     3421 tctgaataaa taaaatttaa tattgttatt taatcccaac tttacaatct aatgatatct
     3481 ctttctatac atattgcata tacaaatgta ataacttgac tattagaata ttttgtttta
     3541 acaatcctat ttcatcataa taatgaagta tatgtacact cacttttgta agttttgcaa
     3601 tccattttac agttttcatt attgtaaatt ttggttaaat tattcctcta aaaatttctt
     3661 tgctacattt gaaaaatcta atccattcga taaaattgca ggatgaccac ctttatcaaa
     3721 caaatacatt ttaccatttt taatcttttc aagaagtaat gaaaaagtat tttcaaaaaa
     3781 atcactagat attagagata caaactcatc ttctctactt cctgttaaaa gcacctctgg
     3841 ttgcatagtt tctagaggct tatgaaaaaa ctttcctata gttttataat gttcaaaaat
     3901 agcttctgta tcattatcta caactttttc ccagttttct ccttgattat aaatataaaa
     3961 agcttttgca ccatcatctt gcttagatgc ttcacgctct attttcacat tttgtacaaa
     4021 tgactctaga ggaacttctc cttcaaaact atctgcaatt accttattca ctaaatcagg
     4081 tctctcaagt gcaacattta atgcaacaag agctcctcca ctactaccaa ttatgtttac
     4141 ttttttatag ttcattgctt ctaaaaaaca tatcacttgc atagcctcat caaaccataa
     4201 gtcagcagaa aatttatcga ccatttgaga ttttccatga cctacaaaat caattaatat
     4261 tactttatat tcatctttat aaaaattgat tatatcatta aacatcttgg aagaggctgt
     4321 atttccatgt aaaaaaagta atggtgtacc atccccaatt tcattatagt agcactgttt
     4381 gtcgttatag ttaaaatatg ccatatattt ccccccttta atatccctat aggtatatat
     4441 ttattactta tatttaaaat gactattccc ttagccactt aaaaatagta taacttagaa
     4501 tgtagataaa atcaacattc cttgctaatt tatctattgt ttgtctaatt tattataatt
     4561 taacttctgt aaaatttctt ttttgtacga aaatattcct tccatttaca acaatcattc
     4621 aaatgaagta taacatgacg agtaggaggc ataagcaaaa ttccagcaat atctttattc
     4681 ccccaaaagt ccaacaacca aatagaatct tcatgcttta ttactcctcc agtcaccaaa
     4741 atcctttcta agtcaattgg agagaccttt cgtttcatat cttcagcttt caaatcctta
     4801 acaacttcta ttgtgttttt tgcaacttca tttcgataac aaagcaattc atcaatattg
     4861 actaaattat cgtatatctc ttttcacttt aaccattttt ttctttatag gtatctcctt
     4921 atattttttt aatatcaaat ctcccttata atttaatata tctacatatg aacataaatc
     4981 ttgaacttca attacaccta tatcatcatt agttatacct tttatattac taaatgcacc
     5041 aactatatct aaaactctta ttttcttctt cttaccagca tttaaatata atttaacaac
     5101 ttcactttct atatttagtt ttatatcttt acttttgtaa gaatttttat caactttttt
     5161 tctctttgat acttttttta ggattccttt tgaatattct tcaaacaaaa ctttgccatg
     5221 tgtaattcta cttttttcta tatcttttaa ttcatttatt ttatatccta tatacgtttc
     5281 tatattctct atatattttt catccttttc agttactata gtaattgcct ttccactatt
     5341 tccttttctc ccagttctac ctattctatg tatatagttt tctttatctt gtggcacatc
     5401 ataatttatt actaaagaaa tatcatcaat atgtattcct ctggacgcta catcactact
     5461 aactaatata ttaaattttt ggtttttaaa atccttaatt acaaaaattc ttctttcttg
     5521 tgataaatct gcatgtaact ctcttattaa aaaaccctct ttactcatct tgttatataa
     5581 tttagatact ttctctttgg tattacaaaa tattatcaca ctctttggat tttcgctgta
     5641 tattaattct tttaaaagga tgtacttctc tcttccattt gccttgatta ttttgtcatc
     5701 tatttgtctt gttttttgat tagtatcgct ctcattttct tctatattta taacactgta
     5761 atctagcata tatttttcac atatatattt tatctcttca tctattgtag ctgaaaaaag
     5821 acttactatt ttctccttag gtaaatttaa taatattttt tccatttgtt caacaaaacc
     5881 tttattaaac attttatctg cctcatctat tactaaatac tttacatttt ccaacttaat
     5941 agaacctcta tttatatggt caagtattct gcctggagta gctacaacaa tatgaaccct
     6001 ttgtttcaat tcagctattt ggtcttttat agattgtttg ccaaatatag cactacatct
     6061 caccttcttt agtctcccta tatctgatat ctcatctttt acttgcaagg ctaattctct
     6121 tgttggaacc actataagag cttgaatatt attgtaatct acatttatat tctcgcataa
     6181 aggtattgca aaacttgctg tctttccact tcctgttttt gacctaacta ctaagttttg
     6241 accttttaat aattttggaa taacttctct ttgtactcta gaaggtatat tatatcctaa
     6301 actcttcaat gacttaagta tcttttcatt taacttaaat ttttcaaatg tatatttcat
     6361 atattaccac cttattatat ttatcttatc tatatatttt tctacattat attattataa
     6421 tacaaggttc tactattttt atattattta ttagactgaa gataaaagta tatactctat
     6481 aaacctatag tttagtagca aattaattaa atttttatat ttaaatacct ccttaaatat
     6541 actgataaat caatattttc aaggaggtat ctcttgtcaa taatcaaact aatctatctt
     6601 ttgaaagttt aaattatact aatacattgt atttttattt atatctaata ttaaaaggta
     6661 ttaatcaata cctaaatcac cataatctca catatttata tctttattca atataccttc
     6721 tgtaatcaat cgtgctatat aatcataacc tgattcattt gctttgttac aatcattcac
     6781 attatcacag aaaaaacttt caatctgtat tgcaactgga tttgtatttc tcaatatata
     6841 taaattttct ctttttttag ctcctctatt ttcaaataat tctccaagtc tattagatac
     6901 tcttttagca tattctaatc cttcagtgcc atagtaaagc acttctgtac cacattgtgt
     6961 tccatcagct ttatttaaat gtagctctac taataaatcg tatttaccac tgtttacttt
     7021 tggaactcta tagaaaaatt cttctctttc tgacataaag tgcctttctg gacaaattat
     7081 tacatctact gtattacctt gacttataag catctcagct aacataggag ctaatttttt
     7141 attataattg tattcatcaa ctataccatt tacacctgta cttttaccac cttttagtat
     7201 actatgacct actgttatgc atattttcat ttactttttt caccttcttt taagccaatt
     7261 tttatataat agtaatataa ttttaaagtt attactaata ataatatatt aattgaagaa
     7321 ggtaaaagtt ctccgtgatt atcatttttt taatatattt gtcttatttt atc
//
LOCUS       DAMJVK010000150         7207 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.155, whole genome shotgun sequence.
ACCESSION   DAMJVK010000150 DAMJVK010000000
VERSION     DAMJVK010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7207
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.155"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(<1..520)
                     /locus_tag="PC414_003526"
     CDS             complement(<1..520)
                     /locus_tag="PC414_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2465878.1"
                     /translation="MIAVKKAYKFRMYPNKKQQELINKTFGCCRFVYNKYLAKRIDVY
                     KNDKETFTYKQCSSDLTNLKKELKWLKEPDKFSLQNALKDLDNAYKKFFKEKAGFPKF
                     KSKKINRFSYKTNFTNGNIMYCGQHIKLPKLGMVKIRDKQVPKGRILNATISKEPSGR
                     YYVSLCCIDVDIE"
     gene            complement(1169..1576)
                     /locus_tag="PC414_003527"
     CDS             complement(1169..1576)
                     /locus_tag="PC414_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465879.1"
                     /translation="MSKVAKATFYLMIVTIISKILGMGRELVLSSIYGTGLYTESYLT
                     AMNIPNIIFAAIGTAIVTTFIPMYQDISSKQGEKQALKFLNNVLNIIVGICIVVAILG
                     VIFSKQLVSIFAIGFEGERFLLTVKFTKILIKK"
     gene            complement(2050..2934)
                     /locus_tag="PC414_003528"
     CDS             complement(2050..2934)
                     /locus_tag="PC414_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2465880.1"
                     /translation="MDTKKENNYENYICVGSSNVSNAMKMYLKEIEEYKMLSAGEEVE
                     LAKEIINSSSVAKEKFINSNYRLVVSIAKRYKRDSIDMLDLIQAGNIGLIKAVEKYDY
                     KKGYKFSTYATWWIKQSITRYIDDCENTIRIPIHLHQRINFVKKKKQELLNVLLREPT
                     IDEIADACGLEVDKVLELLRRDKNVVSLDTPLKEDEDSSLVEFIPSDADFKDVVIHEV
                     EQHNLKEKIEELLTGLGEQEQQVLRMRFGIDDDDPKTLEQIGKVFGVTRERIRQIEAK
                     AIRKLRHPSKLKQLKHFY"
     gene            complement(3225..3701)
                     /locus_tag="PC414_003529"
     CDS             complement(3225..3701)
                     /locus_tag="PC414_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HDF2465881.1"
                     /translation="MSKELVKDRVIKYLIEDLLVPQDMIDTNVELAEFEEGAEGILDI
                     VVNVKDEEDYYAPVMIVQCLDEDVELEGEVLQKQIEFLEEVDNITMSGRLVLTNGDAM
                     MYADWRGEEYDTEAALPTYDIMVKEFHEMEQQVKDLEEHHHHDENCGCGCNHHHEN"
     gene            3967..5142
                     /locus_tag="PC414_003530"
     CDS             3967..5142
                     /locus_tag="PC414_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HDF2465882.1"
                     /translation="MKFKKQISSIVVSTMLVLGSMNLSYADGTNVVTLGANLSESQKQ
                     QMLNYFGVKKDQVLVLDVTNAEERQYLQGVATEGQLGKVTISCSYVEPTKKGNGINVK
                     TANLTWVTSSMIASTLTTAGLEDANVIAAAPYPVSGTGALTGIMKAFEDASGKKLDET
                     KKEIASEELVVTGDLGDDIGQEKATGVMNDIKTEIVKNGTKDTNQIANTINNVVNNYN
                     ITITPEQKEQIKGVMKKIADQNYDYNSMKNTLDNVSDNVNEQLKKLGESVKGSGILDT
                     IGGWFSGIGDWFSGLFSGGDKKDLGILNNTNDESLGSDAVINSTEGVQINPQNNTNSN
                     NDSDTNSSKNDTSSNNENNDSSQTNENNTTNNSENQSNNNDSVGFFEKIKNWLSGLF"
     gene            complement(5223..6026)
                     /gene="proC"
                     /locus_tag="PC414_003531"
     CDS             complement(5223..6026)
                     /gene="proC"
                     /locus_tag="PC414_003531"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDF2465883.1"
                     /translation="MKKIGFIGAGNMASAMIGGIVNSKLVEPNMVIASAYSQGTLDRI
                     DANFGINTTKDSKEVTRTSDIVIVAVKPDIYDDILEEIKDFIDDNKIIVTIAAGKSIK
                     DIESIIGEDKKIVRTMPNTPALVNEAMSSLSINKNINKEDLEAVTEVFNSFGNTEVVP
                     EYLIEAVIGASGSAPAYVFLFIEAIADAAVIAGMPRPQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPGGTTIEAVKVLEEEGFRASVIKAICACIEKSKKMSE"
     gene            complement(6066..6650)
                     /locus_tag="PC414_003532"
     CDS             complement(6066..6650)
                     /locus_tag="PC414_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2465884.1"
                     /translation="MNKDIGAKIKQLRTQKQMTLKDMSEKTNLSIGFLSQLERGLTSV
                     ATDSLGKIASVLDVELTYFFMKPKEHKRAVLRSYEKEVFDVENSTFIHYHLSSSLKEK
                     TMLPRLIEILPSKSSEEICCYVHEGEEFVYVLEGTLTVFLGDEQIEMYPGDTIHYNSE
                     KNNHNWVNYTNKVVKILVVSIPNPFEKSDAVKEA"
BASE COUNT         2365 a         1208 c          803 g         2831 t
ORIGIN      
        1 cttcaatatc tacatcaata cagcataatg atacataata tctaccactt ggttctttag
       61 atatagtagc attaagtatt ctccctttag gtacttgttt atcccttatc ttaaccatac
      121 caagtttagg tagttttata tgttgaccac aatacataat gtttccattt gtaaagttag
      181 ttttatatga aaatctatta attttctttg atttaaattt aggaaatcct gctttttctt
      241 taaagaattt tttatatgca ttatctaaat cttttaaggc gttttgtagt gaaaatttgt
      301 caggttcttt aagccatttt aattcttttt ttaaattggt caaatcagaa ctacactgtt
      361 tataagtaaa agtttcttta tcgtttttat aaacatctat tcttttagca agatacttat
      421 tatatacaaa tctacaacat ccaaaagttt tgttaattaa ctcttgttgt tttttatttg
      481 gatacattct aaacttataa gcttttttca ctgctatcac ctcactttaa ttgttttttg
      541 atacactttt taatgatttt atcacatttt atcacattta atatattata taatgctgtg
      601 gtgaacaatt tattaatcac ctataataat gttaacaaat gtaattattt aaaacaatta
      661 tttagaaggt tttatattta attttaaata acatatttaa gaaccgtaag tcttaacttt
      721 tatcattttt gaggttgtcg ttcacataag ttcgctacta cctatgcagt tctcttatga
      781 acttcttaca ctttcatgta agcacagact atatcttatc cttcagcatt atccgttaag
      841 gtctacccac ttccactacc aatagcttgt agtgtacttc cctcaagagg aatagtcgtt
      901 gaagtttctc ctattcggag tttacctgct gattgcccat tttaaagtac ttaggattta
      961 accttatacc atctaaccaa ttttttctac tttcgtaact ttcacactta ggtttatttc
     1021 atccttatgt tttagtttga ttagctttag ggatttccag caattcaagt agtattggat
     1081 aggttctatc ctatctctac atataggttt ccctatatgc tgactatttt agctactaac
     1141 tcatgactaa agtcacgagt gtgcgtagct attttttaat caatatttta gtaaatttta
     1201 cggtaagtaa aaacctctct ccttcaaatc ctatagcaaa tatacttaca agttgctttg
     1261 agaaaataac tcctaatata gctacaacta tgcatatacc aactattata tttaaaacat
     1321 tgtttaaaaa ctttagtgct tgcttttcac cttgtttact acttatatct tgatacattg
     1381 gaataaaagt agtaactata gcagtaccta ttgctgcaaa tataatattt ggtatattca
     1441 tagctgttaa ataactctct gtatataagc ctgtaccata aattgatgat aatactaatt
     1501 ctctacccat tccaagaatc ttagagatta tagtaactat catcaaataa aaagttgctt
     1561 tagctacctt gctcatttta tcactccact tctaacatac ctctttttaa tcaagtaaaa
     1621 tttagctcat tagatataat tctatcaact aatttgcctt gcttagccct atcaaaatat
     1681 aattaacacc tatttcttaa tatatcacaa actacattat tcttcatatt tttttatttc
     1741 tccaataagt tttacatttt tcctatataa attgtatagc ttcaattata ttcaaagaaa
     1801 tggagttgct acccgataac tttaaaagta aaaaacagat tttattatct ttttaaacta
     1861 tattctaaca tattttgtcc atattgagaa attttactaa actattatgt actttattaa
     1921 ataaataaaa taacagcaca cctataaaat acaagtgtgc tgttcataaa cagattttat
     1981 aaaaaaataa ttcctaagta gagattaaca tgctttctca tactctcata tataatgtgt
     2041 ctaaaaatat taataaaaat gctttaattg ttttaactta cttggatgtc ttaattttct
     2101 tattgcttta gcttctatct gtcttattcg ttctctggta actccaaata cctttccaat
     2161 ctgttctagt gttttagggt catcatcatc aattccaaat ctcattctta aaacttgctg
     2221 ttcttgctct ccaagacctg taagtagttc ttctatcttt tcttttaaat tatgctgttc
     2281 aacttcgtgt attacaacat ctttaaaatc agcatctgat ggtataaatt caactaaact
     2341 actatcctca tcttctttta atggagtatc caaagataca acatttttat ctcttcttaa
     2401 taattccaac actttgtcaa cttctaagcc acatgcatct gcaatctcat ctatagttgg
     2461 ctctcttaat aatacattta aaagttcttg tttctttttc tttacaaaat ttattctctg
     2521 atgaagatgt ataggtatcc taatagtatt ttcgcaatcg tcgatatatc ttgttatact
     2581 ttgttttatc caccaagttg cataagtgct aaatttgtat ccttttttat aatcgtactt
     2641 ttctacagct tttataagtc ctatatttcc tgcttgaatt aaatccaaca tatcaatact
     2701 atctctttta tatctctttg ctatgctcac taccaaccta taatttgaat ttataaattt
     2761 ttcttttgct actgaagatg agttgattat ttccttagcc agctctacct cttctcctgc
     2821 tgacaacatt ttgtattcct ctatttcctt taaatacatt ttcatcgcat tcgatacatt
     2881 tgatgaaccc acacaaatat aattttcata attattttct tttttagtat ccataatcaa
     2941 accccttcta ttcatagaat ttgctaaaac aaaaataaat aatttaaacc tacataatct
     3001 acaattacat aatatcccat tctttaaatg aattgttttg tatattatgt atacaaattt
     3061 ataaaaacta tacctataat attattctac ttctaacagc aatatcctcc ttatatatat
     3121 aatttatttt attatatgtt gttatattct tagttttttg aatgatatat gtctaaaaaa
     3181 agactgttta ctaaacagtc tttttttata tatatgctca atatttaatt ttcatgatga
     3241 tgattacatc cacatccaca attttcatca tggtgatgat gttcttctaa atcttttacc
     3301 tgttgttcca tttcatgaaa ctcttttacc attatatcat aagttggaag agctgcctca
     3361 gtatcgtatt cttcccctct ccaatcagcg tacatcatag catctccatt agttaaaacc
     3421 aatctacctg acatagttat attatccact tcttctaaaa attctatttg tttttgtaaa
     3481 acttctcctt ctaactccac atcttcatct aaacattgca ctatcattac aggtgcataa
     3541 taatcttctt catctttaac atttacaaca atatctaaaa ttccttctgc accttcttca
     3601 aattctgcca actcaacatt tgtatcaatc atatcttgag gtactaataa atcttctatt
     3661 aaatatttta ttactctatc ttttactaat tcttttgaca tttatttttc tccttccgat
     3721 tctataacat tatcataatc atttctatat tatagtatac tacattaatt gttttttcaa
     3781 tctcttacaa aaattatttt ttattatctt ccttatattc ccttttttat tacaaagttg
     3841 taatcgcatt gatttaaaaa atagtttata tttagtataa tagaaatgta gcacatattt
     3901 ttattcaata tattaataaa aaaatatatt gaaaatgtaa tgtatgttaa atctaggagg
     3961 aatattatga agtttaaaaa acaaattagt tctattgttg tgtctactat gcttgtactt
     4021 gggagtatga acctttctta tgcagacggt acaaatgtag ttacacttgg agctaactta
     4081 tcagagtctc aaaagcagca aatgcttaat tactttggag taaaaaaaga ccaagtctta
     4141 gttttagatg taacaaatgc agaagaaaga cagtatttac aaggtgttgc aacagagggt
     4201 caacttggaa aagttactat ctcttgctca tacgttgaac ctactaaaaa aggaaatgga
     4261 attaatgtta aaacagcaaa cttaacttgg gtaactagtt caatgattgc ttcaaccctt
     4321 acaacagctg gtcttgaaga tgcaaatgtt attgctgctg ctccatatcc tgtaagtggt
     4381 actggagcat tgacaggaat catgaaagca tttgaggatg cttcaggaaa gaaacttgat
     4441 gaaactaaga aagaaattgc ttctgaagaa ttggtagtta caggtgactt aggagatgat
     4501 atagggcaag aaaaagcaac aggtgtaatg aatgatataa aaacagaaat tgtaaagaat
     4561 ggtacaaaag ataccaatca aatagcaaat acaataaata atgttgtaaa taattacaat
     4621 attactatta caccagaaca aaaagaacaa attaaaggtg taatgaaaaa aatagctgac
     4681 caaaattacg attacaatag catgaaaaat acactagata atgtatcaga taatgtaaat
     4741 gaacaattaa agaaattagg tgaaagtgtt aagggttctg gtatactaga tactattggt
     4801 ggatggttta gtggaatagg agactggttc tctggtttat tctcaggcgg agataaaaaa
     4861 gatttgggaa tcttaaataa tactaatgat gagtctcttg gctctgatgc tgtaataaat
     4921 tctacagaag gtgttcaaat aaatccacaa aataatacta attctaacaa tgatagtgat
     4981 acaaatagct caaaaaatga tacttcaagt aataatgaaa ataacgatag ttctcaaaca
     5041 aatgaaaaca acacaacaaa taattcagaa aatcaatcaa ataataatga ctcagttggt
     5101 ttctttgaaa aaataaaaaa ttggctttca ggcttatttt agaaattaaa caaataaaaa
     5161 gaattgccta aaatggaaat tttctataaa aggcaattct ctttatttac aataaatttt
     5221 tattactcac tcattttttt agatttttct atacatgcac atattgcttt tatcacagaa
     5281 gctctaaaac cttcttcttc aagtacttta actgcttcta tagtagttcc accaggtgaa
     5341 cataccatat cttttaattc accaggatgt tttcctgtct caagtaccat ttttgcagaa
     5401 cccataacag cctgtgatgc aaacttatat gcttgtggtc ttggcattcc tgcaattact
     5461 gctgcatctg ctatagcttc tataaataaa aatacatatg ctggtgcaga accacttgca
     5521 ccaattacag cttctataag atactctggt actacctcag tatttccaaa agaattaaat
     5581 acttctgtaa ctgcttctaa gtcttctttg tttatatttt tatttatcga aagagaagac
     5641 atggcttcat ttacaagagc tggagtattt ggcatagttc taactatttt tttatcttct
     5701 cctataatag actctatatc ttttatagat tttcctgctg ctattgtaac tataatttta
     5761 ttgtcatcta taaagtcttt tatctcttcc aaaatatcat catatatatc tggtttaaca
     5821 gctacaatta ctatatcaga ggttctagtt acttccttcg agtcttttgt agtatttatt
     5881 ccaaaatttg catctattct atcgagagtt ccttgactat atgctgatgc tataaccata
     5941 ttaggttcaa ctaattttga atttactatt ccccctatca tagcgcttgc catatttcct
     6001 gcacctatga atccaatttt tttcacttta gtacctcctt attgactaat agtaatcgtt
     6061 aaaatttaag cttctttaac tgcatcagat ttttcaaatg gatttggtat acttactacc
     6121 aaaattttca ctaccttatt agtatagttg acccaattat gattgttctt ttcactatta
     6181 tagtgtattg tgtctcctgg atacatttct atttgttcat ctcccaaaaa cactgtaaga
     6241 gtaccctcta atacatacac aaattcctca ccttcatgta cgtaacaaca aatttcctca
     6301 ctactcttac ttggaagaat ttcaattaat cttggtagca ttgttttttc ctttaaacta
     6361 gatgataaat gataatgtat aaatgttgag ttttctacat caaatacttc tttttcataa
     6421 cttcttaata cagctctttt atgctctttt ggcttcataa aaaagtaagt taattctaca
     6481 tctagtacac ttgctatttt tcctaaggaa tctgttgcca cacttgttaa tcctctttca
     6541 agttgtgata aaaaaccaat tgacaagttt gttttttcac tcatatcctt cagcgtcatt
     6601 tgcttttgag ttctgagttg ctttattttt gctcctatgt ccttattcaa aaccatcacc
     6661 cctaaataat tttaattttt tcataatata aatacaaaaa tataaatata ggtactgaac
     6721 ccatctttat ttcatttaaa aaaattttta taagtattac tataatttat caatcttttt
     6781 ttgtctacat ttttacgatt atttacgatt tatttttcac atatgtatat ttaatcttgc
     6841 caactaaaga tacatttttt ataagcattt actctttata taataaaact taagttgttc
     6901 atgtagtctt cttctggttt aattattaaa atttcaaatt atattattat ttctacacat
     6961 tattacatcc tttagtacaa ttataacata attttacaat ttaatgatat ctattcaatg
     7021 tcttttttta ctctacaaca tattttattt tagagtttaa tactatatat attttttata
     7081 tacagccttg tcatttttta gattgtcaag agttattgaa gtattttatg tagatttaga
     7141 ttttatttcc taatttctca tttaacaaga attgcttatg ataaaaaata atatcatctc
     7201 aataaga
//
LOCUS       DAMJVK010000151         7118 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.156, whole genome shotgun sequence.
ACCESSION   DAMJVK010000151 DAMJVK010000000
VERSION     DAMJVK010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7118
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.156"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            11..871
                     /locus_tag="PC414_003533"
     CDS             11..871
                     /locus_tag="PC414_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF2465885.1"
                     /translation="MLLKKDRITFSEILILIVGCILMAISLNLFFNPHAIAAGGITGL
                     GVVLNSLFGVELWIVNLLLNVPLFIFAYKILSKKDCFKTVLGIIFLTIALKLTANMAT
                     LDITNDMYLAIISGSILMGVGQGLIFRINGSTGGTDLMALLLNKYFPTFSIPVLMGIV
                     DCVVVVLSGIVNRQVEIALYSTVALYILVKVSDLLIEGFNYSKSFTIISDLSKDISKK
                     IMEDLDRGATILKGEGAYTGENKNVLLVVVEKKEVVELKKLVKNVDPNAFIIITDIHE
                     ALGNGFKKIE"
     gene            1327..1893
                     /locus_tag="PC414_003534"
     CDS             1327..1893
                     /locus_tag="PC414_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF2465886.1"
                     /translation="MSVSLQKGQRVSLVKDNNPVKNLVVGLGWDMNKLGKKNYDLDAF
                     AIALTNQDKMNVRGDLVYFGNLKHPSKAIIHTGDNLTGKGEGDDEQIIVNLEDIPEYV
                     HKIVFGVSIYKAKKRDQDFGQINNSFIRLIDSNSKQELFKYNLQEDFSGKVTVLAGEI
                     YRRNEEWKFNALGIGQNEELRELINTYK"
     gene            1933..2514
                     /locus_tag="PC414_003535"
     CDS             1933..2514
                     /locus_tag="PC414_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF2465887.1"
                     /translation="MGITLAKGQKVSLTKSNPGLKKVIVGLGWDINKYDGGFDFDLDA
                     SAFLTGTDGKVTNDGDFIFYNNLKHASGAVEYMGDNRTGVGDGDDEQINVDLSKIPQN
                     IAKISFSVTINEAITRRQNFGQVENSYIRIYNEETNEELIKYELGEDFSIETAIVVAE
                     LYRHNGEWKFNALGSGFEDGLAGLCKNFGVNIG"
     gene            complement(2571..3488)
                     /locus_tag="PC414_003536"
     CDS             complement(2571..3488)
                     /locus_tag="PC414_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2465888.1"
                     /translation="MTIRSLEIFVKVAECGKMSEVARNMYITQSSVSQAISEIEKEYG
                     VKLFDRISKKLYLTEAGKKLLGYGRHLLAVNEEMNDCMKHCAKNIRIRVGATVTVGTC
                     VISPIMLELYKVNPLIEPEVFVEDTRLIAKKLLNSELDIAIVEGKIKHPDIVTKSIIN
                     DNLVLICSHKHEFYKRDSIKVSELSNQPLIMRELGSGTRAQLEKQLKELKIPMNIRWS
                     CYNSEAILRAVVDNFGIAVISELLIEDYLKKHLLWACDIEGINLHRTFDIAYHRSKFF
                     TENISAFFDISVEYGKKQARKKSRSVNSL"
     gene            3618..5249
                     /locus_tag="PC414_003537"
     CDS             3618..5249
                     /locus_tag="PC414_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDF2465889.1"
                     /translation="MKVLIIGGVAAGTKTAAKIKREMGDNCSVTILNKGDDISYAGCG
                     LPYYVGKIIEDKSSLIVNTPESFSKLTGAEVRCGVEVTSIDRNAKQVTLKTLSTGEKE
                     NLSYDKLVIATGADPIKPPIPGIDLPGVFFMRTPNDAIKLRDSIENGIKRAVVIGGGF
                     IGLEIAENLSAMGIRVSVVDMAEHVMPGFDTDFAEYVENHMADKGIMIFTGDQVVGIE
                     GENKVEKLRTKNRAIKTDLVVMSVGIRPNTGFLKDTGLEFAPNNTIIADEYMLTNDKD
                     IYVVGDCAFVKNSLTNKPTWSPMGSSANHEGRICAQNISGKSKTYNGVLGTTVVKLPE
                     LNAGKTGLSKEIAEKEGYNVCSVTIATDDKAHYYPGASNFIIRMVAEKNTRKLLGVQV
                     MGPGAVDKIVDIAATAITLGADLYSLESMDLAYAPPFSTAIHPFVVAVNVLQNKLNGE
                     LDSVLLDEIEDFDSWTKLDVSKAPSIPSLRYIPVGEINGEIEGLAKDEKILLICAKGK
                     NSYMAQNRLRRFGYTNTKVLEGGILFYPNLKVMEE"
     gene            5252..6199
                     /locus_tag="PC414_003538"
     CDS             5252..6199
                     /locus_tag="PC414_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013378669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HDF2465890.1"
                     /translation="MSNVTAEQKKEVKGRGFLPSRDGEHFAARIITVNGVINASQAKK
                     IAEAAEKFGNGQLAFTTRLTVELPGVKFEDIEALSEFIASENLITGGTGSRVRPVVAC
                     KGTVCVHGLANTQALAAEIHEEFYKNWYDVKLPHKFKIGVGGCPNNCIKPDLNDLGIV
                     GQRVPDYDSELCVGCKKCAVVEVCPVKAAKLTDKGKLEIDSNLCNNCGKCIESCNFDS
                     IEEKESGYKVYIGGKWGKSVRPGTQIDRLFSKEELMTLIEKAILLFREQGNTGERFGI
                     TIDRIGADKFIEMLLSDEVLERKEEILEALLHLTGGAVC"
     gene            complement(6484..6747)
                     /locus_tag="PC414_003539"
     CDS             complement(6484..6747)
                     /locus_tag="PC414_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1883 domain-containing protein"
                     /protein_id="HDF2465891.1"
                     /translation="MEYSYSKMNLKKGDIVEVNLEKQANVILLDHINYVKFKNQRNYD
                     YYGGFAKKNPCRMRVPNTGTWYLVVNQDGNSGIVNFSINTIQN"
     gene            complement(6846..7091)
                     /locus_tag="PC414_003540"
     CDS             complement(6846..7091)
                     /locus_tag="PC414_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465892.1"
                     /translation="MFIDEELEGYILTCKISEDFKNIPEYSDEEFYVTVYKDESSDSG
                     YYALLENKEERVVWDGEVVANNIFNNLWIVVNKVKTG"
BASE COUNT         2576 a          936 c         1146 g         2460 t
ORIGIN      
        1 ataaggagtg atgttattga aaaaagatag aataactttt tcagaaattt taatattaat
       61 tgtaggctgt atattaatgg ccatttctct taacttattt tttaatccac atgcaatcgc
      121 tgctggtggt ataacaggac ttggagttgt tttaaattca ttattcggtg tagaactttg
      181 gattgtcaat ttacttttaa atgttccatt attcatattt gcttacaaaa tactttcaaa
      241 aaaagattgt tttaaaacgg ttttaggaat aatttttctt accatagcac ttaagcttac
      301 tgcaaatatg gctacattag atataacaaa tgatatgtat cttgcaatta tatctggttc
      361 tattttaatg ggagttggtc aaggacttat ttttagaata aatggctcta caggtggaac
      421 tgatttaatg gcactcctac taaataaata ttttcctaca tttagtatac ctgttttaat
      481 gggtattgta gactgcgttg tagttgtttt atcaggtata gtaaatagac aggttgaaat
      541 tgcactttat tcaactgtgg ctttatacat tttagtaaaa gttagtgact tgcttattga
      601 aggatttaat tattccaaat cattcactat tatatctgac ctttcaaaag atataagtaa
      661 aaaaataatg gaagatttag atagaggtgc aactattcta aaaggagaag gtgcatatac
      721 tggagaaaat aaaaatgtac ttttagtagt agttgaaaag aaagaagttg tcgaattgaa
      781 aaaattagta aaaaatgtcg accctaatgc ttttattata attacagata tacatgaagc
      841 tttaggtaat ggatttaaaa aaatagaata actaacaaat aaatagcaag tataattaac
      901 atatacaaat tcaacttttt atatgttata ttatacttgc tatttatata ttatacttgc
      961 tatttatata ttataattgt tgtttttaag ataaaaatta aatgtatcaa ttattgtttt
     1021 atacaagtat ttccaaatcc gttctcattt agatagtatg cttagaaatt ttagaattat
     1081 tgagtctaac tttattactt actttacttt ataacaatca aatacaaatt taaatattat
     1141 aaaactacaa aataatttaa agcatataat gtcaatatgt ttaaattata aaatattcat
     1201 gtagttatat aataaataat ttataaatat agccaactta gcctaaatac gtatataata
     1261 tagctaatat ggttcttata cttgatacca tcaataaaac taaaaatata aaaaggagaa
     1321 cttaaaatga gtgttagttt acaaaaagga caaagagtta gcttagtaaa agataataat
     1381 cctgtaaaaa atctagtagt aggcttagga tgggatatga ataaattggg taaaaaaaat
     1441 tatgacctag atgcatttgc aatagcattg actaaccaag ataaaatgaa tgttagaggt
     1501 gacttggttt attttggaaa tcttaaacac ccttctaaag ctattataca cactggagat
     1561 aacttaactg gaaaaggtga aggtgatgat gaacaaataa ttgttaattt agaagacata
     1621 cctgaatatg tacataaaat agtttttgga gtttctatat acaaagctaa aaaaagagac
     1681 caggattttg gtcaaataaa caactctttt ataagattaa ttgattcaaa ttccaagcaa
     1741 gaattattta aatataatct tcaagaagac ttttctggta aagtaacagt tttggcagga
     1801 gaaatttatc gtagaaatga agaatggaaa tttaatgctt taggaattgg tcaaaatgaa
     1861 gagcttcgtg aattaattaa tacatataag taataattac aataaaaaat ttataatgag
     1921 gagttggttt aaatgggtat aactttagca aaaggacaaa aagtaagttt aacaaaaagt
     1981 aaccctggtc taaaaaaagt aatagtagga ctaggatggg atattaataa atatgatggt
     2041 ggctttgatt tcgatttaga tgcctctgca tttttaactg gaactgatgg aaaagtaacg
     2101 aatgatggag actttatatt ctataataat ttaaaacacg cttctggagc tgttgaatat
     2161 atgggtgata atagaacagg tgttggtgat ggtgatgatg aacaaataaa tgtagactta
     2221 tctaaaatac ctcaaaatat tgctaaaata tcatttagtg ttactataaa tgaagcaatt
     2281 acaagaagac aaaatttcgg acaagttgaa aattcatata taagaatata taatgaagaa
     2341 actaatgagg aacttattaa atatgaattg ggtgaagatt ttagcataga aacagcaata
     2401 gttgtggcag aactatatag acataatgga gagtggaaat ttaatgcttt aggttctgga
     2461 tttgaggatg gacttgcagg attgtgtaaa aattttggcg ttaatatagg ctaatatatc
     2521 taaataaaaa taagagtata ttgatatact cttattttta tttagatata ttataaactg
     2581 tttacacttc tactcttctt tctcgcttgt tttttcccat attctactga aatatcaaaa
     2641 aatgctgaaa tattttctgt aaaaaacttg cttctatgat atgcaatatc aaatgtccta
     2701 tgtaaattaa taccttcaat atcacaagcc cacaaaagat gcttttttaa ataatcctca
     2761 ataagtaatt cggatataac tgcaatgcca aaattatcta caactgctct taatatagct
     2821 tcagagttat aacatgacca tctaatattc attgggattt ttaattcttt taattgcttt
     2881 tctaattgtg ctcttgtacc acttcccaat tcacgcataa ttaatggttg atttgaaagt
     2941 tctgaaactt ttatagagtc tcttttataa aactcatgct tgtgtgaaca tataagtaca
     3001 agattatcat taataatgct ttttgtaaca atatctgggt gcttaatttt tccttctaca
     3061 atcgcaatat ctaattcact attaagtaat ttttttgcaa tcagtcttgt atcttctaca
     3121 aatacttcag gttctataag aggatttacc ttgtataatt ctaacattat tggacttatt
     3181 acacatgtac caacagttac tgttgcccca actcttatac ggatattttt tgcacaatgc
     3241 ttcatacaat cattcatttc ttcatttaca gctaaaagat gcctcccata tccaagaagt
     3301 tttttccctg cctcagttaa atataatttt tttgaaattc tatcaaatag cttaacacca
     3361 tactcttttt ctatttctga aatagcttgg ctaactgatg actgggtaat atacatattt
     3421 cttgctactt cactcatttt accacattca gcaaccttaa caaagatttc aagactacga
     3481 attgtcatat tatcacaacc ttagcaaaca ttagtaaatg cttatgattt tattaatatt
     3541 ttagtatttt acaaatatat tgtcaagaag tataatttta ttatagagtt ataaagtaga
     3601 ttggagtgtg atatattgtg aaagtattaa ttataggtgg agttgctgct ggaacaaaaa
     3661 ctgctgcaaa aataaaacgt gaaatgggtg ataattgctc tgttacaata ctaaataaag
     3721 gtgatgatat ttcttatgca ggttgtggtc ttccatatta cgttggtaag ataattgaag
     3781 ataaatcatc tttaatcgta aatacacctg aaagtttttc taaactaaca ggagcagaag
     3841 ttcgttgtgg agttgaagtt acatctattg atagaaatgc aaaacaagta actttgaaaa
     3901 cgctttcaac aggagaaaaa gaaaatctaa gctatgacaa acttgtaatt gctacagggg
     3961 cagacccaat aaaaccacca atacctggaa tagatttacc tggagtattc tttatgcgta
     4021 ctccaaatga tgctataaaa ctaagagatt ctattgaaaa cggtattaag agagctgttg
     4081 ttattggtgg aggtttcatt ggtcttgaaa ttgctgaaaa tctttctgca atgggtatac
     4141 gtgtaagtgt tgttgatatg gctgaacatg ttatgcctgg atttgatact gattttgctg
     4201 aatacgttga aaatcatatg gctgataaag gcataatgat ttttacaggt gaccaggttg
     4261 taggtattga aggtgaaaat aaagttgaaa aacttcgtac taaaaataga gctattaaaa
     4321 cagatttagt ggttatgtca gttggaatcc gtcctaacac aggttttcta aaagacacag
     4381 gtttagaatt tgcccctaat aatacaatta ttgcagatga atatatgctt acaaatgaca
     4441 aagatatata tgtagttgga gactgtgctt ttgtaaaaaa ttctttaaca aataaaccta
     4501 catggtcacc tatggggtca tctgcaaatc atgagggtag aatttgcgca caaaatatat
     4561 ctggaaaatc taaaacttac aatggtgttc ttggaacaac agtagtaaaa ttacctgagc
     4621 tcaatgcagg aaaaacaggt ctaagtaaag aaatagctga aaaagaaggt tataatgttt
     4681 gctctgtaac catagctact gatgataaag ctcattatta tccaggtgca tctaatttta
     4741 ttataagaat ggttgctgaa aaaaatacta gaaaattatt aggtgttcaa gttatgggac
     4801 ctggagcggt tgataaaata gtagatatcg ctgctactgc aataacttta ggcgctgacc
     4861 tttattcatt agaaagtatg gaccttgcat atgcaccacc tttttcaaca gctattcacc
     4921 cttttgtagt ggcagtaaat gttttacaaa ataaacttaa tggagaactt gatagtgttt
     4981 tacttgacga aattgaggat tttgacagtt ggactaaact tgatgtttca aaagctcctt
     5041 ctattcctag tttacgttat attcctgttg gtgaaattaa tggagaaatt gaaggtcttg
     5101 ctaaggatga aaaaattctt cttatatgtg ctaaaggtaa aaatagctat atggcgcaaa
     5161 atcgcttaag acgctttggc tatacaaata cgaaagttct tgaaggtgga atactatttt
     5221 atccaaatct taaggtaatg gaggagtaaa tatgagtaat gttacagcag aacaaaaaaa
     5281 ggaagtaaaa ggtagaggtt ttctaccaag cagggatgga gaacattttg cagcacgtat
     5341 aataactgta aatggtgtaa taaatgcatc acaagcaaaa aaaattgcag aagctgctga
     5401 aaaattcgga aacggtcagt tagcatttac tactagacta acagtagaat tacctggtgt
     5461 aaaatttgaa gatattgaag ctctaagtga atttattgct agcgaaaatc taataacagg
     5521 tggaactgga tcaagggttc gtcctgttgt ggcttgtaaa ggtactgttt gtgttcatgg
     5581 tctagctaat acacaggcac ttgctgctga aatacatgaa gaattttaca aaaattggta
     5641 tgatgtaaaa ctacctcata agttcaaaat tggtgttggt ggatgcccta ataactgtat
     5701 taaacctgac ctaaacgatc ttggaattgt tggtcaacgt gtacctgact atgattcaga
     5761 gctttgtgtt ggttgtaaaa aatgtgctgt tgtagaggtc tgtcctgtta aagcagctaa
     5821 attaacagat aaaggtaaac ttgaaataga tagtaatcta tgtaataact gtggtaagtg
     5881 tatagaaagt tgtaattttg atagtattga agaaaaagaa tctggttata aagtctatat
     5941 aggagggaaa tggggaaaat cagttcgtcc tggaacacag attgatagat tattttcaaa
     6001 agaggaactt atgactttaa ttgaaaaagc aattctttta tttagagaac agggtaacac
     6061 aggtgaacgt tttggaataa ctatcgatag aatcggtgct gataaattta tagaaatgct
     6121 tttatcagat gaagttttgg aaagaaaaga agagatacta gaggctcttt tacaccttac
     6181 aggtggagct gtatgctaac aataaatatt ttaattgtac agataaccta tggaaaaaat
     6241 tttagttaag aataattaaa attctatata tgatatatga taaatgaggc tatctcaaaa
     6301 cacagatacg cctctttttc attgttgaaa tgataaagta tatttgagtt catataaaaa
     6361 agagtgtctc atgaattata aaattcattt tgaggcaccc acattatttt ataataattg
     6421 cttattaatt ttattttaac tatataaaat gtacaaaaat taagaatatc caatattcta
     6481 aaattaattt tgaattgtat tgattgaaaa gtttactatt ccactatttc catcttgatt
     6541 tactactaga taccatgttc cagtattagg aactctcatt ctacatggat ttttctttgc
     6601 aaatccacca tagtaatcat aatttctttg atttttaaat ttcacataat ttatatggtc
     6661 taacaatatt acattggctt gtttctctaa atttacctct acaatatccc ctttttttaa
     6721 gttcatctta ctataactat attccattga attactcctc cagtatcgaa taatctttgt
     6781 tatttactca tctcaaatat gtaaaaattc atttaaaata ttacaaatac tatataatta
     6841 gactattatc cagttttaac cttatttact acaatccaaa ggttattaaa aatattattg
     6901 gcaacaactt ctccatccca tacaactctt tcttctttat tttctaataa agcatagtac
     6961 ccagagtcag aactttcatc tttatagact gtaacataaa actcttcatc actatattca
     7021 ggtatatttt taaagtcttc agatatttta catgttaata tataaccttc taattcttca
     7081 tctataaaca aagcaattcc tccaatctct atattttt
//
LOCUS       DAMJVK010000152         7013 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.157, whole genome shotgun sequence.
ACCESSION   DAMJVK010000152 DAMJVK010000000
VERSION     DAMJVK010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7013
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.157"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(136..1071)
                     /locus_tag="PC414_003541"
     CDS             complement(136..1071)
                     /locus_tag="PC414_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDF2465893.1"
                     /translation="MAKQRSKRISINGEPNPENIKLWKQYKRAKQIAGKSEKTIYNYE
                     CDIMQFFKFLNIECFDTLLTDINEEEIEAYIGYCMEQGNNEKRIRRRISSISSLLSFL
                     KKKRKITDNWCEMIERPGTGEEVQKRTFLTEEQLRKLKEKLSEQDNLQLEVYINLGLA
                     TMARSNEISQFRWDNIDLDNRWIHGITAKGGVSRDFRFSEEVQHLLIEWKDYLSQNNI
                     DYPYIFFTRYGGKYNQVNSNVLSSWVKKAFRLIGIEGGYNHDLRHSMSNLLKDRGVPI
                     TTVSKLLGHSGVDVTINHYTIENKNKLAEEYDKFM"
     gene            complement(1105..1416)
                     /locus_tag="PC414_003542"
     CDS             complement(1105..1416)
                     /locus_tag="PC414_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465894.1"
                     /translation="MLSKEMVELLSQYGYTAILLMVLMLWLGKYLEKNRQLEQDDRKK
                     ERAYFSREIKEQRTLFGNTIDKFDDKLDKFAEALNTNNSRLERVETDITKIKDKLETR
                     D"
     gene            complement(1417..1668)
                     /locus_tag="PC414_003543"
     CDS             complement(1417..1668)
                     /locus_tag="PC414_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDF2465895.1"
                     /translation="MDIMQFIPENLIMLIGGLYILGTFIKESNIKNKYIPFILLVIAM
                     ISSCLFMKELSIEAVFEGILCWGASIGINQIQVQNRKDK"
     gene            complement(1680..2297)
                     /locus_tag="PC414_003544"
     CDS             complement(1680..2297)
                     /locus_tag="PC414_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDF2465896.1"
                     /translation="MNILDKSTATLEQVFSYLDTLKNNSNPPHFLCRAIVPIIYKEAE
                     KKGVNPVIAIAQAFVETGYFNFGRVLNPSYCNVCGLKGNKGGGDLDPTAHTRFNCWED
                     GVSAFIDHLALYAGAKGYPKYNELVGKVEYKVNGTTLDPRHFSYLHGEAKTVESLSGK
                     WCPDTNYGQNIINICNKISSMKVENNDIKLEQIKTKVKELNEILG"
     gene            complement(2309..3124)
                     /locus_tag="PC414_003545"
     CDS             complement(2309..3124)
                     /locus_tag="PC414_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2465897.1"
                     /translation="MNIKTLTIHAGHNPDNKIGSGAIGNIKESTEARNVLKELLPLAQ
                     KECKVYDCTCNNGTSQSDILNKIIAKCNSYNTDLNVSIHFNSGGGRGVEVLVYNLNDK
                     ETVEIASRICKKITETYHAKGDKDFKNRGVKEKKTLAFLRRTKAKSILVECCFVDTSD
                     TKKYNTKDMAIDIYEGIFNKSVAGKPQDNKVKYAIVYEGDVDKVIAQLMAMNYKTNEV
                     SVCDLKNYVPGHCENLYVIGGASSKIKTSERFTKLQGDDRWATLHKVLDFIGK"
     gene            complement(3205..3375)
                     /locus_tag="PC414_003546"
     CDS             complement(3205..3375)
                     /locus_tag="PC414_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465898.1"
                     /translation="MYNILKRMIEQKNYETREELQTKLDVFYAMNRIKESEYTELTNL
                     LNKEDTLVEPII"
     gene            complement(3401..3721)
                     /locus_tag="PC414_003547"
     CDS             complement(3401..3721)
                     /locus_tag="PC414_003547"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465899.1"
                     /translation="MDIEKKIEILIEYGFENDLNNIDNYFIAEGKKIFTPVIKNGELI
                     KTGEQVYNEWIELQNNPPKPSIEEINADKITILIENQKQQDSLLVDNAYRIAMLELNT
                     NNVL"
     gene            complement(3736..5637)
                     /locus_tag="PC414_003548"
     CDS             complement(3736..5637)
                     /locus_tag="PC414_003548"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465900.1"
                     /translation="MIYKDLLKKVNDIENLKEVDNIKCSTDTGEYTIENSKKGYLTNF
                     NVEGKTLIDLWGKTSSDFSLWKATFVDGKINILTENDIRYSNFFTINYTSYKPDTIYT
                     IIVDVDKNTLPSTSGIYIHSLGEENSVFIPNLTNIAIPGGVIGKFKYTFTTISDLNDC
                     NVVLRSVLDNDTLTSGYEISLKITILEGDYTDINIDYSNELLSVGQRDKIEFLSYQYS
                     GINIFNKNADFKDNYILQYLSGEELISEASNHKYTLDYIEIEPDTEYTFYNCSRNICW
                     YDINKNFIPASLNERIIGDKDIFYIARSPKNAKYLRVTIIKDLHDNGNKTIITKGNKY
                     DKKTIPYTLRSLPNGVKDEIVYKNDKYYLIKRCEEHTYTDIGNLNLSHVYDNTLQFMG
                     TLTPQAVVDSLDTAYVLCNNLNGKSRNDFNNNDIEGCSTTGSGDIAFKILKSKLTTQD
                     GNGFSEWIKNNPITIIYQLSKPQEIELTSLNLEQYNNQTKFICNTGIVIPDISFESTQ
                     NLGSHIEVIRNNIKNYNVRTDFPFSINFLNGWQPYLGYASSVGNYCTNNNLVTINATI
                     NGGITTAGTIIGKIPSKYAPRKGIIVIFQTTDGKYYNGIIRTNGEIEIYYNDITYRSW
                     LYLYVNYLI"
     gene            complement(5665..5847)
                     /locus_tag="PC414_003549"
     CDS             complement(5665..5847)
                     /locus_tag="PC414_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465901.1"
                     /translation="MNINNVVVRILAERILSRGLNPLKNRPFELDDVTNEDYRKAVED
                     CIIKESGVVERVETTK"
     gene            complement(5847..6140)
                     /locus_tag="PC414_003550"
     CDS             complement(5847..6140)
                     /locus_tag="PC414_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465902.1"
                     /translation="MNRANRIIYDQTGKILLQTGEATGDILEHDTITELHYIDVEYGN
                     IDYSKQYIESINPITKEPILKDIPIYLSEEEKRIQELENQILLNENEKVGGLL"
     gene            complement(6154..>7013)
                     /locus_tag="PC414_003551"
     CDS             complement(6154..>7013)
                     /locus_tag="PC414_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465903.1"
                     /translation="LTKIEYFIDDILKETITTDLTTEKTINYTLEDNALHTLKIVVTD
                     SNNTTAEKVLSISKNIMPLPENVNLNDISTKLVEVNAGFKVGKTSIINTLALKNIEAS
                     LNNTLVELSEKIKTSFDSSDASVQDLMNQLTQANNTISQLNSKYKYATGTAYARENSS
                     LIACVYDPNTSHTVTETSPYWLDLNGIGFIPDIFFAECEYEPHSNAFYKYFVFAIKNT
                     FPISNNTGFVVNIAFNKEYGDESFKLRGALYTLGKRHVSMDNNGVRVPSLSTLNDFRA
                     YKWHAIKFK"
BASE COUNT         2124 a         1210 c          736 g         2943 t
ORIGIN      
        1 aacatatata aaaaaataaa gccctctaaa gaaaactcaa ttaagagtaa tccctagagg
       61 gctttattat ttatctatct ctttttctta ccttttttac gacctttaca tgccatgata
      121 ccacttcctt tcaatttaca taaacttatc atattcctca gctaatttat ttttattttc
      181 tattgtgtag tgatttatag ttacatcaac cccactgtgg ccaagcaatt ttgaaactgt
      241 agttattgga actcctctgt cttttaaaag attagacata ctatgtctaa ggtcatgatt
      301 atatccacct tctataccaa ttaacctgaa agctttttta acccatgaac ttaagacatt
      361 tgaattgact tgattgtatt tgcctccata tcttgtaaag aatatatacg gataatctat
      421 attattttga gatagataat ccttccattc tattaataga tgctggactt cttctgaaaa
      481 tctaaaatct cttgaaactc cacctttggc tgtaatacca tgaatccatc tattatctaa
      541 atctatattg tcccatctaa attgacttat ctcattactt ctagccattg tagccaatcc
      601 taaatttata taaacttcta actgtaaatt gtcttgctct gaaagctttt cttttaattt
      661 gcgtaactgt tcttcagtta aaaaagttct tttctgaact tcttcgcctg ttcctggtct
      721 ttctatcatt tcacaccagt tgtcagtaat tttcctcttc ttctttaaaa atgatagcaa
      781 tgatgaaatt gaacttattc tacgtctaat tcttttttca ttatttccct gttccataca
      841 ataacctata taagcttcta tttcttcttc atttatatca gttagtaatg tatcaaaaca
      901 ttctatattt aaaaacttaa aaaattgcat tatatcacat tcataattat atattgtttt
      961 ttcacttttc ccagctattt gttttgctct tttgtattgc ttccataatt taatattctc
     1021 tggatttggt tctccattta tgcttattct tttactgcgt tgttttgcca aatacttcac
     1081 ttccttataa aaagactaga gaaattaatc tctagtctct aatttatctt taatttttgt
     1141 tatgtctgtt tcaactcttt caagtctact gttattagta tttaatgctt cagcaaattt
     1201 atctaattta tcatcaaact tatctattgt attaccaaat aaagttctct gctctttaat
     1261 ttctcttgaa aaataagctc tttccttttt tctatcatct tgttctagtt gtctattttt
     1321 ctctaaatat tttccaagcc ataacatcag taccattaaa agtatagctg tatatccata
     1381 ttgacttagt aattcaacca tttcttttga taacatctac ttatcctttc tgttttgcac
     1441 ttgtatttga tttattccta tagatgcacc ccaacataaa ataccttcaa agacagcttc
     1501 tattgacagc tctttcataa acaaacaact acttatcatt gctataacta acaatataaa
     1561 aggtatgtat ttatttttaa tattagattc ttttataaat gttcctaata tgtaaagccc
     1621 acctattaac attattaaat tttcaggtat aaattgcatt atatccatgt gatttcctcc
     1681 tatccaagaa tctcatttaa ttcttttact ttagttttta tttgctctag ttttatatca
     1741 ttgttttcta ccttcattga acttatctta ttgcatatat ttattatgtt ttgaccatag
     1801 tttgtatctg gacaccattt acctgataag ctttcaactg ttttagcttc tccatgtaga
     1861 tatgaaaaat gtctagggtc taatgtagtt ccattgactt tatattcaac tttaccaact
     1921 aattcattgt attttggata tcccttagct ccagcatata atgctagatg gtcaataaat
     1981 gctgatactc catcctccca acaattaaat cttgtatgag ctgttgggtc taaatctcct
     2041 ccacctttat ttcctttaag accacacaca ttgcaataag aaggatttaa aactctacca
     2101 aaattaaaat atccagtttc tacaaatgct tgagcaattg caataactgg atttacacct
     2161 tttttctctg cttctttata tataattggt actatagctc tgcataaaaa atgtggtgga
     2221 ttagagttat tttttaatgt gtctaaatac gaaaagactt gttctaatgt agctgttgat
     2281 ttatctaata tattcaattc attacctcct acttgcctat aaaatctaac actttatgaa
     2341 gtgtagccca tctatcatcg ccctgtaact tagtgaatct ctcgctagtt ttaattttac
     2401 tggatgctcc accaatcaca tataggtttt cacaatgtcc aggaacataa tttttcaaat
     2461 cacacacaga gacttcatta gtcttataat tcatggccat taattgggct ataactttat
     2521 ctacatcacc ctcataaact attgcatatt tcactttgtt atcttgtggt ttaccagcta
     2581 cagacttatt aaatattcct tcataaatat ctatagccat atctttagta ttatattttt
     2641 tagtgtcaga tgtgtctaca aaacagcact caactaaaat tgactttgcc ttcgttcttc
     2701 ttaaaaatgc tagagttttc ttttctttaa ctccacgatt tttaaaatct ttatcacctt
     2761 ttgcatgata agtttcagtt atttttttac atattcttga tgctatttca acagtttctt
     2821 tgtcatttaa attataaact aaaacttcta ctcctcgacc accaccactg ttgaaatgga
     2881 tacttacatt tagatcagta ttatatgaat tacatttagc tataatttta ttcaatatat
     2941 cactttgaga agttccattg ttacaagtac agtcataaac tttacattcc ttttgagcta
     3001 aaggtaataa ctcttttagt acatttcttg cttctgtaga ttcctttata tttcctattg
     3061 ctccagaacc tatcttatta tctggattat gtcctgcatg aattgttaat gtttttatat
     3121 tcaaaattac cactcctttt aattaaaaga gaattgaaat taatcaatcc tctaaaattg
     3181 acataaaaaa agaaccttta tactttagat aataggttct actagtgtat cttctttatt
     3241 taataaattt gttaactctg tgtattcact ttcctttatc ctgttcatag cgtaaaatac
     3301 atctaactta gtttgcaatt cttctctagt ttcataattc ttttgttcaa tcattctttt
     3361 taaaatatta tacatgttgt tccctccatt ttttataatt ttataacacg ttatttgtgt
     3421 taagctctaa catagcaatt ctataagcat tatctactaa taaactgtct tgctgttttt
     3481 gattttctat taaaattgta attttatccg catttatttc ctctatgctt ggtttaggtg
     3541 gattattttg taattctatc cactcattat aaacctgttc tcctgtttta attagctctc
     3601 cgttttttat cactggagta aatatttttt taccctctgc aataaagtaa ttatctatat
     3661 tatttaaatc attctcaaat ccatattcta ttaaaatttc tattttcttt tctatatcca
     3721 tcttattttt cctttctaaa ttaaatagtt tacatataaa tacaaccaac tacgataagt
     3781 tatatcatta taatatatct caatttctcc atttgtacgg atgattccgt tataatactt
     3841 tccatctgtt gtttgaaata ttactattat gcctttacga ggtgcatatt ttgaaggtat
     3901 ctttccaatg atagtacctg ccgttgtaat acctccatta atagttgcat taattgttac
     3961 aaggttatta tttgtgcaat agtttccaac agaactagca tatccaagat atggctgcca
     4021 cccattaaga aagtttattg aaaatggaaa atcagttcta acattgtaat tttttatatt
     4081 atttctgata acttctatat gacttcctaa attttgtgta ctttcaaaac taatgtctgg
     4141 tatcacaata cctgtgttgc aaataaattt agtttgatta ttatattgtt ctaaatttag
     4201 tgatgttaat tcaatttctt gtggttttga aagttgatag atgatagtta ttggattatt
     4261 ttttatccat tcactaaacc catttccatc ttgtgtagta agtttacttt ttaatatttt
     4321 aaatgctata tcaccactac cagtagttga gcatccctct atgtcattat tgttaaaatc
     4381 atttctactt ttcccattta aattattaca taaaacatat gctgtgtcta aactatctac
     4441 aacagcttga ggagttaatg ttcccataaa ttgcagagta ttatcatata catgtgataa
     4501 attaagattt ccaatatcag tataagtatg ttcctcacat ctttttatta agtaatattt
     4561 atcattttta taaactattt catcttttac tccatttggt aaacttctta atgtatatgg
     4621 aattgtcttt ttatcatatt tatttccttt agttataatt gttttattcc cattatcatg
     4681 taaatctttt attatagtaa ctcttagata ctttgcattt ttagggcttc tagcaatata
     4741 aaaaatatct ttatctccta ttattctttc gtttaatgat gctggtataa aatttttatt
     4801 tatatcatac caacaaatat ttctactgca attataaaaa gtatactctg tatctggttc
     4861 tatttctata tagtctaggg tatatttatg attactagct tcagaaataa gttcctctcc
     4921 actaagatat tgtaaaatat aattatcttt aaaatcggca ttcttattaa agatattaat
     4981 tccactatat tgataactta aaaattctat tttatctctt tgcccaacac ttaatagttc
     5041 attagagtaa tctatattaa tatctgtata atctccttct aatatggtaa ttttcaaact
     5101 tatttcataa ccagatgtaa gtgtatcgtt atctaaaaca ctccttaaaa caacattaca
     5161 atcatttaag tctgatattg ttgtaaatgt atatttaaat ttaccaatta ctcctcctgg
     5221 tatagctata tttgtcagat taggaataaa tactgaattt tcttcgccta agctatgaat
     5281 atatattcca cttgtactcg gtaaagtgtt tttatcaaca tcaactatga ttgtatatat
     5341 ggtatctggt ttatatgaag tgtaattgat tgtaaaaaaa ttagaatatc ttatatcatt
     5401 ttctgttagt atatttattt ttccatccac aaaagttgct ttccataaag aaaaatctga
     5461 actagttttt ccccataaat caattaatgt ctttccttct acattaaaat tagttaaata
     5521 gcccttttta ctattttcta tagtatattc tcctgtatca gtactacatt ttatattgtc
     5581 aacctccttg agattttcta tatcattgac ctttttaagt aaatctttat atatcaaatt
     5641 aaaacacctc tcttcacaaa acccctattt tgtagtttct actctttcta ctactccact
     5701 ttctttaatt atacaatctt ctactgcttt tctataatcc tcgttagtta catcatctaa
     5761 ttcaaaaggt ctatttttta aaggattcaa tcctctactt aaaatcctct ctgctaatat
     5821 tcttactaca acattattta tattcattat aatagccctc ctactttttc attttcattt
     5881 agtaatattt gattttctaa ctcttgtatt ctcttttctt cttcgcttaa atagattgga
     5941 atatctttta aaataggttc ttttgttatt ggatttatag attctatata ctgtttacta
     6001 tagtctatat ttccatattc aacatcaata taatgtaatt ctgttattgt atcatgctct
     6061 aatatatctc ctgttgcttc tcctgtttgg agtaatattt taccagtttg gtcgtaaatt
     6121 attctatttg ctctattcat tttatcacct catttattta aattttatcg catgccattt
     6181 atatgctcta aaatcattta gagtgcttag tgaaggtact ctaactccat tattatccat
     6241 ggaaacatgt cttttgccaa gggtatataa agctcctcgt aatttaaaag attcatcacc
     6301 atattccttg ttaaaagcaa tattaactac aaacccagta ttattagaaa tgggaaaagt
     6361 atttttaatt gcaaaaacaa aatacttata aaaagcattc gaatgtggtt catattcaca
     6421 ttcagcaaaa aatatatcag gaataaatcc aattccattt aaatcaagcc aataagggct
     6481 cgtttctgta acagtatgag aagtattagg gtcatataca catgcaatta aggatgaatt
     6541 ttctctagca taagcagttc ctgttgcata tttatactta gaatttaact gtgatatagt
     6601 attattagct tgtgttaact ggttcatcaa atcctgcaca ctagcgtctg aactatcaaa
     6661 acttgttttt attttctctg acaactcaac aagtgtatta tttaaacttg cttctatatt
     6721 ctttaatgct aaagtattta taatacttgt tttcccaact ttaaatcctg cattaacctc
     6781 aactaatttt gttgatatat catttaaatt tacattttcg ggtagtggca ttatattctt
     6841 acttatactt aatacttttt ctgctgtagt attattactg tctgtaacaa ctattttaag
     6901 tgtgtgtagt gcattatctt ctaatgtgta gttaattgtt ttctctgttg ttaaatctgt
     6961 tgttatagtt tcttttagta tatcatctat aaagtattct attttagtta ata
//
LOCUS       DAMJVK010000153         6865 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.158, whole genome shotgun sequence.
ACCESSION   DAMJVK010000153 DAMJVK010000000
VERSION     DAMJVK010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6865
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.158"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            714..1019
                     /locus_tag="PC414_003552"
     CDS             714..1019
                     /locus_tag="PC414_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465904.1"
                     /translation="MPSMIMEVVGVLFIAIGLILTFIEIKGNFPSFYGFGQNSIGKNA
                     VGIIAAGIALIFFRNGIDSIDSIATYGVFLAIELVVIVAYFLIRKKFTKEDKHLRTN"
     gene            1427..2116
                     /locus_tag="PC414_003553"
     CDS             1427..2116
                     /locus_tag="PC414_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2465905.1"
                     /translation="MDILLVEDDKNIREMVYDFLISENYSVKTAIDGNTAIEIFKNYS
                     FDIILLDLMLPKSSGFEVLKYIRSVSVVPVIITTAKDSDTDKMLGLNLGADDYITKPF
                     SIVELLARIKANIRRATVYSSSRNSEDMVIQYKDLIINISKYVVSKNGIDLNLTHTEF
                     EIVKLLATNQGRAFSKEQLYNAIWKEPYYGNENVLNTHINRLRNKLSDGIEKNNYIKT
                     LWGIGYKMEEE"
     gene            2116..3033
                     /locus_tag="PC414_003554"
     CDS             2116..3033
                     /locus_tag="PC414_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2465906.1"
                     /translation="MELFLVVISIVLFALYLKERNKSKKMESDYKYINSRLRDITNDD
                     EINYILVPSDISIVKETAVDINKLLEKFYSRQINYNRSEKAMLQIFTNISHDLRTPIT
                     VLKGYIEMLYLQSQKEELSSSMRATVEKMQTNSNELVNSINNLFNMAKIQSGDMILNL
                     QKSNLTQLCHEIILEFYDLLDKENFNVEVNIEDRPIYANVDTEALQRILKNLIDNAIK
                     YGSDGRFLGISLYEKEKHVFIDIEDKGSGISETEKEHIFTRAYTADRKKGNGLGLAIS
                     QGLVNSMGGSIYVNSRPNVKTVFTVKLKS"
     gene            3106..4032
                     /locus_tag="PC414_003555"
     CDS             3106..4032
                     /locus_tag="PC414_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2465907.1"
                     /translation="MNYIIETNQLKKIYKDKAVVNAVNIHVKKGEIYGFVGPNGAGKS
                     TVMKMLLNLVKPNSGEIVMFGKKVAETDFEILKKIGTIIENPYLYENLTAKQNLDLHC
                     EYMGYYNKEHISEVLECVGLSKQSNKKVSKYSLGMKQRLAIARAILTKPELLILDEPI
                     NALDPEGIREMRELFRKLNTDYGITIFISSHILSEVEQIADTIGIIQDGKLIKEISMS
                     DIHKYQTDYVEVDVDNVELAGYLLEKEFGIKDFKITSESCIEIYDLRKDVKEISKIFI
                     QNQIGINSIGRKQSSLEDYFFQTTGTGGKETL"
     gene            4029..4709
                     /locus_tag="PC414_003556"
     CDS             4029..4709
                     /locus_tag="PC414_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2465908.1"
                     /translation="MIHLMKLELKKVGLKRYFLFSMLGIFLCMFFVFTGLNDKSTSIY
                     DYDVTFRTISLFFCFYYIILFAVLNVAYIINEYTNKTILILFSYPLDRKKLILSKLLL
                     ITTLMISSMIVGYICCFTFVVVLDKYFNLVIGDFHISILSYWIPGAIKSIIVFCSLGI
                     WTFVVGMIRKSIPVTLVSSIIFINLRQFILAGTNTTQDSLISVFIIVGITVIGIYYIL
                     NHKVTEID"
     gene            4807..5142
                     /locus_tag="PC414_003557"
                     /pseudo
     CDS             4807..5142
                     /locus_tag="PC414_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427885.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
     gene            6126..6776
                     /locus_tag="PC414_003558"
     CDS             6126..6776
                     /locus_tag="PC414_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2465909.1"
                     /translation="MKVKSNLDEILYHKIIESLIRGEYSVGQKILLNDLCEKFEVSRT
                     PVVQAVKMLNKDGVLTIMTNGKVYVPEYEYDMVKQVCETRTLIETYALEKMMQEEEEV
                     FQQKLDVIKRYSDKCEEYYRQEKSVELALADLELHKAIVEGANNEILNDVYVGIQGRF
                     IVVNYLIRPLKNRNYEGTVQDHFEILKFIEDRDTKKAVDKLRNHIQGTIKRFCEDE"
BASE COUNT         2756 a          687 c         1063 g         2359 t
ORIGIN      
        1 atagattaga gaataaatga cttataaata aaaaccaagc taatagatta gagaatgaat
       61 taattatagg taaaaaccaa gctaatagat tagagaataa atgacttata aataaaaacc
      121 aagctaatag attagagaat gaattaatta tagataaaaa tcaagctcat agattaaaaa
      181 ataaatgatt tatagatgaa gaactaaact tatagattaa aaaataaatg acttatttat
      241 agatgaaaaa ccaaacttat agattaaaaa ataaatgatt tatagatgaa gaactaaact
      301 tatagattaa aaaatgactt atttatagat gaaaaaccaa actcatagat taaaaaataa
      361 atgacttata gatgaaaaat caagctcata gattaaagaa taaataactt attagatgaa
      421 aaaccaagct tatagataag aagtccatgc ttataagcca gaaaatgaat actttaatag
      481 attaaaaaat acatatttgt gaattataga atgaatgact ccataaacta gaaaatttat
      541 aataatatct atataaaaat tagaagtatt ttatcacaaa aaaattaagt taaataaata
      601 tgaaattact tccaaatata cttccaaaaa catgtaatcg catatataat ataattataa
      661 aaataataaa tatttttata gtagtagtta attattaata aggaggggtc aaaatgccta
      721 gcatgattat ggaagttgta ggagtactat ttatagctat tggattaatc ttgacattca
      781 ttgaaataaa aggaaatttc ccttcttttt atggttttgg tcaaaattct atagggaaaa
      841 atgctgtagg tattatagca gcaggaattg ctttaatttt ctttagaaat ggcatagata
      901 gcatagattc tatagctact tatggtgtat ttttagcaat agaacttgtt gtaatagttg
      961 catatttctt aataagaaaa aaatttacta aagaagataa acatttaaga actaattaac
     1021 taaattatct actataaaat tggaaaatta aaaacaaata aaagtttgtt tttaattatt
     1081 taaaatccaa atttgattat ttttaaagaa actatgttct gtataaaact agaatgtggt
     1141 ttctttttta tgtataaaat attttttagc aagagttaaa aggtgtttta taatataaaa
     1201 tagttaattg tagatgttaa taaaagaaaa ttatgtatat ttcagataaa gataagagag
     1261 tatactatat tttaattatt ttttcaataa agcttattga aaagaacttt aacttagaag
     1321 agtatcacca aattgcagat acaatatcat aagcagttat ttattgacaa ccctttttaa
     1381 taaatatatc atttgaagta tactgacgaa aaggagttga ataataatgg acatcttatt
     1441 ggttgaagat gataagaata ttcgagaaat ggtatacgat tttctgatta gtgagaatta
     1501 ctctgttaaa actgccattg atggaaatac agctattgaa atatttaaaa attattcatt
     1561 tgatattatt ttactggact tgatgttacc aaagtctagt ggttttgaag tattaaaata
     1621 tatacgaagt gtgagtgttg ttcctgttat tattactact gcgaaagata gtgacacaga
     1681 taaaatgcta gggcttaatc ttggtgcaga tgattatatt acaaagcctt tttcaatcgt
     1741 agaattgctt gcaagaatta aagctaatat tagacgtgca acagtgtaca gcagttcaag
     1801 aaactctgaa gatatggtta ttcagtataa agatttgatt ataaatatat caaaatatgt
     1861 agtttcaaag aatggaattg acttaaactt aacacataca gaatttgaaa ttgttaaatt
     1921 actagcgact aatcaaggac gtgctttttc aaaggaacaa ttatacaacg ctatttggaa
     1981 agaaccttat tatggaaatg aaaatgtgct aaatactcat attaacaggt taagaaataa
     2041 attgtctgat ggtatagaaa aaaataatta tattaaaact ctttggggaa ttggctacaa
     2101 aatggaggaa gaataatgga gttattttta gttgtaattt cgattgtact atttgctctc
     2161 tacttaaaag aacgaaataa atcaaagaaa atggaaagtg attataagta tatcaattca
     2221 agacttcgtg acataacaaa tgatgatgaa attaattata ttttagtacc atctgatatt
     2281 tcgattgtaa aagaaacagc agtagatata aataaacttc ttgaaaagtt ttacagcagg
     2341 caaattaatt ataatagaag tgaaaaagcg atgttgcaaa tatttacaaa catatcacac
     2401 gatttacgaa ctcccatcac agtattaaag ggttatattg aaatgttata tcttcaaagt
     2461 caaaaagaag aattatcttc ttcaatgaga gcaactgtgg aaaaaatgca aacaaattca
     2521 aatgagcttg ttaattcaat aaataacctg tttaatatgg caaaaatcca atctggagat
     2581 atgatattaa atcttcaaaa atccaatcta acacaattat gtcatgagat tattttagag
     2641 ttttatgatt tgctagataa agaaaacttt aatgttgaag ttaatattga agatagacct
     2701 atatatgcaa atgttgacac agaagcatta caacgtatac tcaaaaattt aattgataat
     2761 gctataaaat atggttcaga tggtagattt ttagggattt cactatatga aaaggaaaaa
     2821 catgttttta tagatattga ggataaaggt tcaggtattt ctgaaactga aaaagagcat
     2881 attttcacaa gagcttatac tgctgacaga aaaaaaggca atggtttagg tttggctatt
     2941 tctcaagggc ttgtaaactc tatgggaggt tcaatttatg taaatagtag acctaatgta
     3001 aaaacagttt ttacagtaaa attgaaaagt tagaaaaaag ttagatttaa aagagagaaa
     3061 agataatttt attttgtata ctgtttttag aaaggaggga gtatgatgaa ctatatcata
     3121 gagaccaatc aattaaagaa aatatataaa gataaagctg ttgttaatgc tgttaatatt
     3181 catgtgaaaa agggagaaat ttatggattt gttggaccaa acggggcagg taaaagtaca
     3241 gtaatgaaaa tgttgctaaa tcttgttaag ccaaattctg gagaaatagt tatgttcggt
     3301 aagaaagtgg cagaaactga ttttgaaata ttaaaaaaga ttggtactat tattgaaaat
     3361 ccgtatttat atgagaattt gactgctaaa cagaatcttg atttacactg tgaatatatg
     3421 ggttattata acaaagaaca tatatcagag gtattggaat gtgtcggatt gtcaaagcaa
     3481 tcaaacaaga aagtttcaaa atattcttta gggatgaaac aacgtcttgc aatagctaga
     3541 gcaattctta ctaaaccaga attattgatt cttgatgaac ctataaatgc actcgaccca
     3601 gaggggataa gagaaatgcg agaattattc cgtaagttaa atacagatta tggaataact
     3661 atatttattt ctagtcatat tctttcagag gtagaacaaa ttgcggacac tattggaatt
     3721 attcaagatg gtaaacttat taaagaaatt tctatgtcag atatacacaa atatcaaaca
     3781 gattatgttg aagtagatgt agataatgtg gagcttgctg gatacttgtt agaaaaagaa
     3841 tttggaataa aagactttaa aattacttcg gaaagttgta ttgaaatata tgatttacga
     3901 aaagatgtaa aagaaatatc caaaatattt attcaaaatc aaattggtat aaatagcatt
     3961 ggacgtaaac aaagttcttt agaagattac ttcttccaaa ctacaggtac aggaggtaaa
     4021 gaaacactat gatacattta atgaaactag agctaaaaaa agttgggcta aaaagatatt
     4081 ttttattttc aatgctgggg attttcttat gtatgttttt tgtctttact ggattgaatg
     4141 ataaatctac ttcaatatat gattatgatg ttacatttag gacaatcagt ttatttttct
     4201 gtttttacta tattatatta tttgctgtgt tgaatgtggc atatataata aatgaatata
     4261 caaataaaac aatcttgatt ttattctctt atccacttga ccgtaaaaaa cttattttat
     4321 caaagttact tttgattacg actcttatga tatcatcaat gatagttgga tatatctgtt
     4381 gttttacatt cgttgttgtg ttggataagt attttaattt agttattgga gattttcata
     4441 tttcaatttt atcttattgg ataccaggag caattaaaag tattattgta ttttgctcat
     4501 taggtatatg gacattcgtt gttggaatga taagaaaatc aatacctgtt acccttgtca
     4561 gttcaattat ttttatcaat ttaagacaat ttattttagc tggtacaaat acaactcaag
     4621 acagtttgat ttctgttttt attatagttg gtataactgt gattggtata tattatatac
     4681 taaatcataa agttactgaa attgactaga tactgtggat aggaaggtag ttattaattg
     4741 tatcttctcg ggataataat gatttgctat tatatcagct attattaata ttattagagt
     4801 tttaataata ttgcacaacc atttgaagtt gagccaaagc ttgatgaatt aattgaatgg
     4861 tactatagtc agagtgaagt atctattaaa gttataactg aatttcatta tagatttgaa
     4921 ttgatacatc catttcaaga tggtaatggt cacataggta gatttgttat gctaaaacag
     4981 atgttagaaa ataatcttcc tataaaaatt gtatcttggg atagtgagga attgtataga
     5041 aactccttaa attcttgtag tttaggtaat tatgtccctt taattgaata tttatcaagt
     5101 ttagaagatt ttagagaagt atataaaatg ttatggaaat aattgtttgc agcaaaaaaa
     5161 gtttatatat aaatgtattt ttatctatgc tatttaacgc taatttctta gttattctta
     5221 gcttggacta tacccactat tttaactata taaaatagtg gatatagttt aactatctct
     5281 ttataactat ttttatgtat cttagttgtt tcttaatatg gggacagttc ataaaactaa
     5341 aagtgatttt taattttatc atttataatt taccattaca tcagaaaaat gcttatcatt
     5401 agaattttca taaaaggtac ctattttttt caattaactg aatcaattct attagtctta
     5461 atctttcata tttcaaattg ataatataag aaaataaaaa ataaaaaatt ttaaattttt
     5521 tatttattgc gaaaaaaata aaaaggtgct atactttttg taacatatta ttcaaattat
     5581 taagagatag taaacattta tagtttaatt tgtagtacac tagcatgatt tgcagaacaa
     5641 tgaattaatc tatagtataa tagtttaatt taaagtacaa cgatttaatc tatagtacaa
     5701 taacttgatt tgaagtacaa cgatttaatc tatagtacaa taacttgatt tgcaatacaa
     5761 tgaattaatc tatggtataa tagtttaatt tgaagtacaa tgaattaatc tatagtataa
     5821 tagtttaatt tgaagtacaa cgatttaatc tatagtataa tagtttaatt tgaagtacaa
     5881 tggtttaatc tatagtataa tagtttaatt tgaagtataa cgatttaatc tatagtacaa
     5941 taacttgatt tgcaatacaa tgaattaatc tatagtataa gagcatgatt tacagtacaa
     6001 taatttaaat tgtagtacaa aagtactatc aaataagaat ttctacttta aaaatattac
     6061 taaaagtaat taaagtccag taattcaata taatatggca aaagaataga aataaggtga
     6121 gagtagtgaa agtaaagtca aatttagatg aaattttata tcataagatt attgaaagtc
     6181 ttattcgtgg tgaatactct gtaggtcaaa aaatattact aaatgacctt tgtgaaaaat
     6241 ttgaagtaag cagaacacca gttgtacaag cagtaaaaat gctaaataaa gatggagtgc
     6301 ttactattat gacaaatggt aaagtgtacg taccagagta tgaatatgat atggtaaaac
     6361 aagtttgtga aacgagaaca ctgattgaaa catatgcttt agaaaaaatg atgcaagaag
     6421 aggaagaggt ttttcaacaa aagctagatg taataaaaag atactctgat aaatgcgagg
     6481 agtactatcg tcaagaaaaa tctgtagaac ttgcactagc agatttggaa ttacataaag
     6541 ccattgtaga aggggcaaat aatgagattt taaatgatgt atatgttggt atacaaggaa
     6601 gatttatagt agtgaactat ttaattcgac cattaaaaaa tcgtaattat gaaggaacag
     6661 tacaagacca ttttgaaata ttaaaattca ttgaagacag agatacaaaa aaagcagtag
     6721 ataaactaag aaatcatatt caaggaacta ttaaaagatt ctgtgaagat gaataataag
     6781 ttttgaagtt actatatata cttatctatt aagttgctaa atgtagtcta agtataatct
     6841 aattatagtt taaacataat ctaaa
//
LOCUS       DAMJVK010000154         6830 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.159, whole genome shotgun sequence.
ACCESSION   DAMJVK010000154 DAMJVK010000000
VERSION     DAMJVK010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6830
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.159"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            318..1151
                     /locus_tag="PC414_003559"
     CDS             318..1151
                     /locus_tag="PC414_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HDF2465910.1"
                     /translation="MRNKKKKINRKKLYLLLSAIVVCLAFIVVGVRVSSLNMKENEIS
                     RNAKLSSSTITDIVSNTSIEVSKGPSKSSGKIAYITIDDGPSKFTDQMIKTLNKYNVK
                     ATFFMIDGNMKEYPQQVKNIIKNGNTAGFHSVSHDIHKLYVTSTSAKEEFDTNDQTFY
                     KITGKHSKVIRIPYGSKPYTPQASYQALVDAGYKIWDWDLDTEDWRSNSSQIVQNVKN
                     HIKNRKGEDKDQLVVLMHEKKQSAEALDSVLKFLSDEGYEFAPVDQNQIPKNYWLRNL
                     E"
     gene            1244..2176
                     /locus_tag="PC414_003560"
     CDS             1244..2176
                     /locus_tag="PC414_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF2465911.1"
                     /translation="MVGILSLMMVGCNKSLAKVNDVEITKEQYKKTKAVLSATNNYIN
                     GQSLDELEKTLDKKGRNKLENVIISFMVDNELLYQEAKDKGLTPSKSEVDSKYQELED
                     KMNLNTSYKEKMDKAGVDKDYLKQEISRDLAIDKNKKAFEDRINISDNDMEAYYTSHK
                     KDFNVEEVSASQILISTLDKNKKEVSKDKKEALKKKADNILTKIKNGESFESLAKKYS
                     DDKATGKNGGQLGYFTKDDKNAEFTKEVFKLKKNEVSNVFETSYGYHIVKVTDKRERQ
                     KSFNECQSLIRESILNEKYIEHIKKLNEDAKIDR"
     gene            2228..2710
                     /locus_tag="PC414_003561"
     CDS             2228..2710
                     /locus_tag="PC414_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2465912.1"
                     /translation="MFKSLKRTKVEKYIIDNKDSFYRIAYSYTKNEEDALDVVQEAMY
                     KALYSVENIKEVNYIKTWFYKILVRTSIDFIRKNRKYNNMTDIDLIDETGEYDKYTDL
                     DLRRALEELPIEYKSIIILRFFEDLKIEEVAIILDENVNTVKTRLYTALKKLKLKIEE
                     "
     gene            2722..3591
                     /locus_tag="PC414_003562"
     CDS             2722..3591
                     /locus_tag="PC414_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HDF2465913.1"
                     /translation="MTSKERLNKLKDEYHKIPIPKKLDTIINHEKIENIYREKNKKVN
                     RLRFKVAIAFACIFTVLVNISPVFADNFSKIPVIGAIVEVITIKNYSLKSENYEAEID
                     IPKIRGLKDKNLEQRLNSSFMEDGKRLYHQFQERMEKIQSSKNKGYKSLSLSYSVKNN
                     SKKFLSIEMTKNEIEASSYVSKVHYTIDKKRQIVLTLPMLFKDDKYIKVISDNIKEQM
                     REQMKKDSTKSYFIDQKKDLPVEDFKTINKYQDFYFNKNEDLVICFDEYEVAPGYMGA
                     VEFVIPYKVIKDL"
     gene            3634..4389
                     /locus_tag="PC414_003563"
     CDS             3634..4389
                     /locus_tag="PC414_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HDF2465914.1"
                     /translation="MTTYKKLKKSIFKIAIFSICLLASLVDTNPIFAGSSFNETSNSD
                     TIKVLNAKSINFKQDNYEVNVSIPKIKGLKDKTLENKLNNEFLQDGKKIYSEFQNKMK
                     KEDNKGHRYLNVIHNVKNNSKDFLSIEMTKEEIEASSSITKKHYTVDKNRGILLNLPI
                     FFKDSRYIRAISDNIKEQMRNQIKKDDTKSYFIDQDKDSPVKDFESINKYQDFYFNKN
                     GNLVISFDEYEVAPGYMGAVEFVIPNKVIKRLR"
     gene            4431..5252
                     /locus_tag="PC414_003564"
     CDS             4431..5252
                     /locus_tag="PC414_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase accessory factor"
                     /protein_id="HDF2465915.1"
                     /translation="MNIYEQAIRLIENNEDFAFATITSHSGSTPRETGAMMIVKNDST
                     IFGSVGGGSVEAECIKHAINVIKNRESMLYKFTLNKSDVAKLGMICGGTGEIQIDFID
                     SKLKSNIEKFNKRLKENTSKAYIFGAGHISRDVAVILSLLEFRTVVIDDREEFANHER
                     FPESEVVVLDSFENIPDFPTDENSYIIILTRGHLYDSSALEWALKREAGYIGMIGSRT
                     KIGLTYEKLMKKGFKKEELSKVHAPIGIKLNAQTPAEIAVCIAAELINCRANKEK"
     gene            5257..6018
                     /locus_tag="PC414_003565"
     CDS             5257..6018
                     /locus_tag="PC414_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HDF2465916.1"
                     /translation="MNTGSKDIDTLKVNFRGIEIKAVLVYKNRKNISIKIDPFGKITV
                     MSPPKISKKIIKDIIIEKGDWILKKSEKCKGREEVYKQRMFITGEKFLYLGEEYHLVI
                     KELLRDSVKKSNCRITINEYQIVVQTNDTSTDFIKKSLKSWYKMESERIVLERIDFLK
                     LNCEIMKQLVPASVKVKEQNKRWGSCTAQKNIYINSKISMARLDVIDYVIIHEFSHLV
                     HMNHSKKFYDLVKSIMPDYKDKENWLKINGYKIII"
     misc_binding    6179..6436
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6549..6788
                     /locus_tag="PC414_003566"
     CDS             6549..6788
                     /locus_tag="PC414_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HDF2465917.1"
                     /translation="MENTVYAKLNQGIDSFLRVAMTLRRREVDIQSISMTVDNMNNSG
                     IKLIVNEEKTSLDSVLNHMKKLHDIREITVERMRH"
BASE COUNT         3000 a          637 c         1153 g         2040 t
ORIGIN      
        1 tggcaaatag ttgttttgct atttattatt tgtttaatat ttctaattta taaaaaaaaa
       61 attataaatt ttaaaaatta taaatgacat tttaactaga attaattgta aaaaacacta
      121 tataatactc aattttcaac attctataca attatacaga aattatacag cataataatc
      181 accgaaataa caaattttaa aaattaagca aaactttttg tcgaagttca acgtctatat
      241 atatgtagtg taaatttttg ggataaaata ctacaaaaat ataaatgtaa aaatatagta
      301 atattggagg tcgaacgatg agaaataaaa agaaaaaaat aaatagaaag aagttgtatt
      361 tgcttttaag tgctattgta gtgtgtctgg cttttatagt agttggtgtt cgcgtcagct
      421 ctttaaatat gaaggaaaat gaaattagta gaaatgcaaa actgtcaagt tctactatta
      481 cagatatagt atctaataca tcaatagaag ttagtaaagg gccaagtaaa agttctggaa
      541 aaatagcata cataactata gatgatggtc catctaaatt tactgaccag atgataaaaa
      601 ctttaaataa atacaatgtt aaagcaactt tttttatgat agatgggaat atgaaggaat
      661 atccacagca agttaaaaat ataattaaaa atgggaacac agcaggtttt catagtgtat
      721 cacatgatat tcacaagctt tatgttacta gtacatcagc aaaagaggaa tttgatacaa
      781 atgatcaaac tttttataag ataactggta aacattcaaa agtaataaga ataccttatg
      841 gaagcaaacc atatacacca caagcctctt atcaggcttt agttgatgct ggatataaaa
      901 tttgggactg ggacttagat acagaagact ggagatctaa ttcttctcaa attgtacaaa
      961 atgtaaaaaa tcatattaag aatagaaaag gtgaagataa agaccaactt gtagttttaa
     1021 tgcatgagaa aaaacagagt gcagaagctt tagattcagt tttaaaattc ttatcagatg
     1081 aaggttatga atttgcgcca gtagatcaaa accaaatacc aaaaaattat tggttacgta
     1141 atttagagta gttaaaatat aagaatattg attgatattt ttagaaattg agaataaaaa
     1201 tgagtgaggt gtaggtatat tgaataaaaa actttatata ggaatggtcg gaatattgtc
     1261 tttgatgatg gttggttgta ataaaagttt agcaaaagta aatgatgtgg aaatcacaaa
     1321 agaacagtac aaaaaaacaa aagcagtttt atctgctact aataattata taaatgggca
     1381 gtctttagat gaattagaaa aaactttgga taaaaaaggt agaaataaat tagaaaatgt
     1441 tataatatcg tttatggtgg ataatgaact tttatatcaa gaggctaagg ataaaggact
     1501 aactccaagt aaaagtgaag tagactcaaa atatcaagaa ctagaagata agatgaattt
     1561 gaatacaagc tataaggaaa aaatggacaa agcaggtgtt gataaagatt atttaaaaca
     1621 agaaatatct agagatttag caatagataa aaataaaaag gcctttgaag atagaataaa
     1681 cataagtgat aatgatatgg aagcctacta tacaagtcat aaaaaagatt ttaatgtaga
     1741 agaagtaagt gcttctcaga tacttatatc gactttagat aaaaataaaa aagaagtaag
     1801 caaagataaa aaagaagctt tgaagaagaa ggcagataat atattgacta aaatcaaaaa
     1861 tggagaaagt tttgaaagtt tggctaaaaa atattcggat gataaagcaa ctggaaaaaa
     1921 tggaggacag ctaggatatt ttacaaagga tgataaaaat gctgagttta caaaggaagt
     1981 gttcaaatta aagaaaaatg aagtttcaaa tgtgtttgaa actagttatg gatatcatat
     2041 agtcaaagtt actgataaaa gagaaagaca aaaaagtttt aatgagtgtc agagtttaat
     2101 aagagaatct attttaaatg aaaagtatat tgagcatata aaaaaactga atgaagatgc
     2161 taaaatagac agatagaatt tctaacagct caatcctagc ctgtaatcgg agggagattt
     2221 tacaggaatg tttaagagtt taaagagaac gaaagttgag aaatacatta tcgataataa
     2281 agactctttt tatagaatag catatagtta tacgaagaat gaagaagatg ccttagatgt
     2341 ggttcaagaa gccatgtata aggcactata ctcagtggaa aatattaaag aagtaaatta
     2401 tataaagaca tggttttata aaattttagt tagaacgagt atagatttta ttagaaaaaa
     2461 ccgtaaatat aataatatga cagatattga ccttatagat gagactggag agtatgataa
     2521 atatactgat ttggatttaa gaagagcttt ggaagaatta cctattgaat acaaatctat
     2581 tataatatta agattttttg aagatttaaa gattgaagaa gtagcaataa tattggatga
     2641 aaatgtgaat actgttaaaa ctaggttata tactgcttta aaaaaactaa aattaaaaat
     2701 tgaagagtag ggataaaaag aatgacaagc aaagaaaggt taaataagtt aaaagatgag
     2761 tatcataaaa ttccaatacc caagaaatta gatactataa taaatcatga aaaaatagaa
     2821 aacatatatc gagagaaaaa taaaaaagta aatagattaa gatttaaagt tgcaatagcc
     2881 tttgcatgta tatttacagt attagtaaat ataagtcctg tatttgcaga caatttttct
     2941 aagatacctg taattggagc aatagtagaa gtaataacta taaaaaatta tagtttaaaa
     3001 agtgaaaatt atgaagctga gattgatatt cctaaaatac gaggtttaaa agataaaaac
     3061 ttagaacaaa gattaaacag tagttttatg gaagatggaa aaaggcttta tcatcaattc
     3121 caagagagaa tggagaagat acaatcatca aagaataaag gatataaatc tttaagtttg
     3181 agttatagtg taaaaaataa ttctaaaaaa tttctatcaa tagagatgac aaaaaatgag
     3241 atagaggctt catcatatgt gagtaaggta cattatacta ttgataaaaa aagacaaata
     3301 gttttgactt tgccaatgct ttttaaagat gataagtata taaaagttat aagtgataat
     3361 ataaaagaac aaatgagaga acaaatgaaa aaggatagta caaaatccta ttttatagat
     3421 caaaaaaaag acttgcctgt tgaagatttt aaaacaatta ataagtatca ggatttttat
     3481 tttaataaaa atgaagacct tgtaatttgt tttgatgaat atgaagtagc acctggttat
     3541 atgggcgcag ttgagtttgt aataccatat aaagtaataa aagatttgta aattgaatag
     3601 tagtttatat ataaatcttt taaggaggac aatatgacta catataaaaa acttaaaaaa
     3661 tctatattta aaattgctat attttcaata tgcttattag cttcattagt agatacaaac
     3721 cctatatttg caggtagttc ctttaatgaa acttcaaata gtgatacgat aaaggtgtta
     3781 aatgctaaga gtatcaactt taaacaagac aattatgaag tgaatgtgag tattcctaaa
     3841 ataaaaggtt taaaagataa aactttagag aataaattaa acaatgagtt tttgcaagat
     3901 gggaaaaaaa tttacagtga atttcaaaat aaaatgaaaa aggaagataa taagggtcat
     3961 agatacctaa atgtaatcca taatgtcaaa aataattcta aggacttttt atcaatagaa
     4021 atgacaaaag aagagataga agcttcctca tctataacta agaagcatta cactgttgat
     4081 aaaaatagag gaatactatt aaatttacca atatttttta aagatagtag atatattaga
     4141 gctataagtg ataatataaa agagcagatg agaaatcaaa ttaagaaaga tgatacaaaa
     4201 tcttatttta tagaccaaga taaagactcg ccagtgaaag attttgaatc tataaataag
     4261 tatcaagatt tttattttaa taaaaatgga aatcttgtga ttagttttga tgaatatgaa
     4321 gtagctcctg gttatatggg agcagtagaa tttgtgatac caaataaagt aataaaaaga
     4381 ctaagataga tatatagaag atttttagat agactggtaa ggagaattat atgaatatat
     4441 atgaacaagc aataagatta atagaaaata atgaggattt cgcatttgct acaataacgt
     4501 ctcattcagg ttcaacacca cgtgaaactg gagcaatgat gattgtgaaa aatgattcta
     4561 ctatatttgg ctcagtagga ggaggtagtg tagaagcaga atgtataaaa catgcaatta
     4621 atgttataaa aaacagagaa tcaatgttgt ataaatttac tctaaacaaa tctgatgttg
     4681 caaaactagg aatgatatgt ggaggtactg gagaaataca aatagatttt atagatagta
     4741 aattaaaaag taatatagaa aaatttaata agagactaaa ggaaaataca agtaaagcat
     4801 atatatttgg tgcaggtcat atttcaagag atgttgctgt gatactatct cttttagaat
     4861 ttagaacagt agtaatagat gatagagaag aatttgctaa tcatgaaagg tttccagaat
     4921 cagaagtagt agttttagat tcttttgaaa acataccaga ttttccaact gacgaaaata
     4981 gttatataat tattttaaca agaggtcatc tatatgattc aagtgcttta gaatgggctc
     5041 ttaaaagaga ggctggttat ataggtatga ttggaagtag aacaaaaatt ggattgactt
     5101 acgaaaaact catgaaaaaa ggttttaaga aggaagaatt atcaaaagta catgcaccaa
     5161 taggcataaa attgaatgct caaacaccag ctgaaatagc tgtatgtata gctgctgaac
     5221 ttataaattg tagagctaat aaggaaaaat aaactaatga acacagggag taaagatatt
     5281 gatactttaa aggttaattt tagagggatt gaaataaaag cagttttagt atataaaaat
     5341 agaaaaaata tttctataaa aatagatcca tttggcaaaa taactgtaat gagtcctcct
     5401 aaaatatcca aaaaaataat aaaagatata attattgaaa aaggagactg gatacttaaa
     5461 aagtcagaaa aatgtaaagg aagagaggaa gtatataaac aaagaatgtt tataactggc
     5521 gaaaaatttc tttatttagg tgaagagtac catttagtta taaaagaact gttgagagat
     5581 tctgtcaaaa aaagtaactg tagaataact attaacgaat atcaaatagt tgttcagaca
     5641 aatgatacaa gtacagattt tattaaaaaa tctttaaaat cttggtataa gatggaaagt
     5701 gaaagaatag tattagagag aatagatttt cttaaattaa attgtgaaat aatgaagcaa
     5761 ttagttccag cgagtgttaa ggtaaaagaa caaaataaga gatggggaag ttgtacagct
     5821 caaaaaaata tttatataaa ctcaaaaata tctatggcaa gattagatgt aattgattat
     5881 gtaataatac atgagtttag ccatttagta catatgaatc actctaaaaa attctatgat
     5941 ttagtaaaat ccattatgcc cgattataaa gataaagaaa attggctaaa aataaatgga
     6001 tataaaataa taatctgaaa acgattattc caaaatatag gcgtaaagta taaaagaagt
     6061 attattgtga aaaaaataaa taatctgaaa attttaaaaa aagtcattga cattgtaaat
     6121 gatagggaat ataatctata ttaacaacaa aacagaatta aaatataaaa ttaaaaaatt
     6181 aaaagcaatg acagagacaa caatactaat agtgttatcc acagagaacc aatgttgctg
     6241 agaattggtg ttaacagtag tataatgatc actctggagc ttttgtactg aatattttta
     6301 gtaagtacaa acgtatgtca gcgttacatg actaaggtta aggtaattaa ttacccgacc
     6361 tgaaaaaaga ggggtagatg tattttttat atctacaaat aaggtggtac cgcggaatat
     6421 aactttcgtc cttataagta atatttatat tgcttataaa gatgaaagtt tttttatttt
     6481 aagcgatata aataaattta atccatataa aattaataaa aaaataataa aaataaggag
     6541 ggatttttat ggaaaatact gtatatgcta aattaaacca aggaatagat tcttttttaa
     6601 gagtggctat gactctaaga agaagagagg ttgatataca atcaataagt atgactgtag
     6661 ataatatgaa taattcaggt ataaaattaa tagtaaatga agaaaagaca tccttagatt
     6721 ctgtcttaaa ccatatgaaa aaactacatg atataagaga gataactgtt gaaagaatga
     6781 gacattaatt aaatatattt tataaagcta aagataaata tgcttatttt
//
LOCUS       DAMJVK010000155         6825 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.160, whole genome shotgun sequence.
ACCESSION   DAMJVK010000155 DAMJVK010000000
VERSION     DAMJVK010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6825
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.160"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            73..1815
                     /locus_tag="PC414_003567"
     CDS             73..1815
                     /locus_tag="PC414_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloendopeptidase"
                     /protein_id="HDF2465918.1"
                     /translation="MVKSKKKIFVFLILIAVFIIFASYIRNYTNDTKKDSVRGTIPFE
                     KMEYKRPNIQSICENINKCNDKLLTAKTAKEQLNLFNEVDKIYQDFYSTLTIAKIRNN
                     IDSSDEFYSKEYKYLMSKSVDVDMSYKDFQDRFVTSKFSKELEKELGKDTFNYLKNIG
                     KLNSKEVEGLLKKEQDLVVKYEDLLAKSTVSIDGIEVDFEEALSRPNLSPEEYVKIYS
                     DYLKKYNPIFGNIFLELIQTRTEIASKQGFKNYIDYAYMNLNKDYSQEEAKKFRQDVK
                     DYIVPLYREISSKPSDSSIYIKVYKNRSFRKFDKVLEDISPKLKESFDYMKKYDLYDY
                     SSGKNKSPGGYTTYINKYKAPFLFNTWDNSFLGVTSFAHEFGHFYNYYNSINLNNKMQ
                     PSIDVCEVHSTSLEILFYKYFDDFFGKQSEAIKKEHLSIVLNTIIDACLYDEFQEIIY
                     KNPYMSLNDINKLFFDLEEEYGVSNNILREKNAPFWILVSHNFQVPFYYLSYGLASDV
                     SLQIWQLSQEDYRKAVDVYMDFLNQNTDAGFKDVVEKVNLQLPFQDGNLEEISSTLYD
                     YFGIDNPLELKNAS"
     gene            complement(1985..2173)
                     /locus_tag="PC414_003568"
     CDS             complement(1985..2173)
                     /locus_tag="PC414_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465919.1"
                     /translation="MKIITIGSSLITVLLFLSTMICGFWIKNNKVTDASSIKFHMNSA
                     IFTGIFLLISTILLIIYI"
     gene            complement(2170..2751)
                     /locus_tag="PC414_003569"
     CDS             complement(2170..2751)
                     /locus_tag="PC414_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2465920.1"
                     /translation="MKSNDKDARERIIEVTLNLLNEVDDIEEITVRKIAERANVGVGL
                     INYHFKTKDNLLSTAIGDVMSNIIAELYDDSVYTLRPIEDLKNLLKKLCDTGLHYEKV
                     LPFVLNQCITNGDMQAELDIVPMLRKIFGNKKDEMSLRIIALQIILPIQISALSTESF
                     QLYSGINIKNKYERDKFIDILIENIIGEDVDVR"
     gene            2919..3737
                     /locus_tag="PC414_003570"
     CDS             2919..3737
                     /locus_tag="PC414_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2465921.1"
                     /translation="MFKIGDFSKLSKISIRMLRHYDEIGLLTPSHTNKTNGYRYYSAD
                     QLSTTNRIHALKDMGFGLYSIKEILTEYNDKESLIKYLNIHHSQVKEQLEDTQKKLLK
                     IETTIKRIGGNDIMKNYDVTIKNFAPKYMMTLRRVIPTYQDEGMLWHQAFLETKDQNV
                     QIEPPKYSKAVFYDTGYKEDYVDVEVQVAVSGKYKDTEHVKFKTVPSVTAATAIVNGN
                     FNQVADACEAIGNWISDNNYDVDGPMFNIYHVSPGNDSNPDNWVTEVCFPVKKK"
     gene            4747..5568
                     /locus_tag="PC414_003571"
     CDS             4747..5568
                     /locus_tag="PC414_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallohydrolase"
                     /protein_id="HDF2465922.1"
                     /translation="MKLKIVGSGGMSIIPSSFCKCKICEEARQKGGRYERLGPSLYID
                     DIKMLIDTPEDIAVACNRQKISEVKHISISHHDPDHVKGIRIVEKIGCDFITGENNPI
                     GFYALPEVIEDINRTNLDCLKYYGDVINCISINETSHVKINNFDIDLINNNPDRNITF
                     YVIKENEKKVIYACCNPKPFTHSDMYFDADILIISLVSDDGILNDGTKLKDTPFKDEI
                     FTLDEIVEIKNYYRIKKIIVTHIDEMWGKSYGYYSELEKKLDNIYFAYDGMEIIV"
     gene            complement(5878..6657)
                     /locus_tag="PC414_003572"
     CDS             complement(5878..6657)
                     /locus_tag="PC414_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HDF2465923.1"
                     /translation="MSYKKIAIITGANSGFGKEFLKLLINEEEITEIWAVARNKARLN
                     QLVDEFGSKVKIFSKDLSKIEEVKEIGTFLSKENVCIKYLINNAGFAKFCSYNDLSID
                     ESINMIDLNISAVVALGLICIPYMQKGSHIMNISSQASFQPLPYQNIYSSTKAFIRNY
                     TRALNVELKSKGINAIAVCPGWMSTNLFKRGIVVAEKGTKNFPGIVSPDVVAKKALKD
                     AKKNKDISVYGINTKSSHLLAKLLPQKMMMKVWLMQQHIKE"
BASE COUNT         2557 a          910 c          874 g         2484 t
ORIGIN      
        1 ttcttaacat gcatttttta tatttaatat agtaaaataa ttaataacta tatccatgat
       61 tggaggattt ttatggttaa atccaagaag aaaatatttg tttttctaat tttaattgca
      121 gtttttatta tatttgcatc atacataaga aattatacta atgatacaaa aaaagattct
      181 gttagaggaa ctattccttt tgaaaaaatg gaatataagc gtcctaatat acagtcaatt
      241 tgcgaaaaca ttaacaagtg caacgacaaa cttttaacag ctaaaacagc taaggaacaa
      301 ttaaatttat ttaatgaagt tgataaaatt tatcaagatt tttacagtac acttactata
      361 gcaaaaatta gaaataacat agattcatca gacgaatttt actctaagga atataagtat
      421 cttatgagta aatcagtaga tgttgacatg tcatacaaag actttcaaga tagatttgta
      481 acttctaaat tctctaaaga attagaaaaa gaacttggaa aggatacatt taattatcta
      541 aagaacattg gaaaacttaa ttctaaagaa gttgaaggtt tgcttaaaaa agagcaagac
      601 ctagttgtaa aatatgaaga cttattagct aaatctacag tatcaataga tggtatagag
      661 gtagactttg aagaagcctt gtcaagacca aatcttagcc ctgaagaata tgtgaaaatt
      721 tattcagatt acttaaaaaa atacaatcct atatttggta acatattctt agaacttata
      781 cagacaagaa ctgaaatagc aagtaagcaa ggatttaaga attatataga ttatgcctat
      841 atgaacctaa acaaagacta ttctcaagaa gaagctaaaa aatttagaca agatgtaaaa
      901 gattacatag ttccattata tagagagatt tcatctaaac catctgattc ttctatttat
      961 attaaagtat ataaaaatcg ctcatttagg aagtttgata aagtacttga agatatatct
     1021 ccaaaactaa aagagagctt cgactatatg aaaaaatatg acttgtacga ttattcttct
     1081 ggaaaaaaca aatcacctgg aggatatact acttatataa ataaatataa agcacctttc
     1141 ttgtttaata catgggataa ttctttttta ggtgttacta gttttgctca tgagtttggt
     1201 catttttata attactacaa cagtattaac ttaaacaata agatgcaacc aagtatagat
     1261 gtatgtgaag ttcattcaac atctttagaa attctatttt ataaatattt tgatgatttt
     1321 tttggtaaac aatctgaagc tataaaaaaa gaacacttat cgattgtttt aaatactatt
     1381 attgatgctt gtctttatga tgaatttcaa gaaattatat acaagaatcc atatatgagt
     1441 ctaaatgata tcaataaatt gttttttgat ttagaggaag aatatggagt ttccaataat
     1501 atacttagag aaaaaaatgc tcctttttgg atacttgtat ctcataactt ccaagttcct
     1561 ttttattact tgagttatgg tcttgcatct gatgtatcac ttcagatatg gcaattatca
     1621 caagaagatt acagaaaagc tgttgatgta tatatggatt ttttaaatca aaatactgat
     1681 gctggattta aagatgtggt agaaaaggta aatttacaat tacctttcca agatggaaat
     1741 ttagaggaaa taagctctac tttatatgat tattttggta tagataatcc gcttgaacta
     1801 aaaaatgctt cttaatttct aatactttat actcactctt aagatttagt attagattga
     1861 aataagtgat attttttcac acacaattaa tgtttaataa aattatatgg taaagtatca
     1921 gaaattttta agactcttgt ctttataatt tctgatactt tatttattaa taatattatt
     1981 atttttatat atatataatt aaaaggattg tggaaatcaa taaaaatatc cctgtaaaaa
     2041 tagcactatt catatggaac ttaatagaac ttgcatctgt taccttatta ttcttaatcc
     2101 agaacccaca aatcatagtt gaaagaaaaa gtaaaacagt gataagactt gaaccaatag
     2161 ttataatttt catcttacat caacatcctc tccaataata ttttctatta aaatatcaat
     2221 gaatttatct cgctcatatt tattctttat atttatacca ctgtataact gaaatgattc
     2281 tgtagataaa gcagaaatct gtattggtaa aataatttgc agggcaataa ttcttagact
     2341 catttcatct tttttatttc caaaaatttt acgaagcata gggacaatat caagttctgc
     2401 ttgcatatct ccattggtta tacattgatt caatacaaaa ggaagcactt tttcataatg
     2461 taatccagta tcacacaatt tttttaataa attttttaaa tcttcaatag gtcttagagt
     2521 atatacacta tcgtcatata gctctgctat tatatttgac atcacatctc ctatggcagt
     2581 actcaacaaa ttatcctttg ttttaaaatg ataatttatt aaaccaactc caacatttgc
     2641 acgctcagca atttttctaa ctgtaatctc ctcaatatca tctacttcat ttaaaagatt
     2701 caatgtaact tcaattatac gttctctagc atctttatca ttacttttca taatagacct
     2761 ccaaaacaat taacattttc agttctgaac atgttcagta taatattttt ttcatcatat
     2821 gtcaatataa tttaattata attttagttg actctaacat tatgatatgg tttatattaa
     2881 tattaagcta aaagtacctt tataaaggag acaaaaacat gtttaagata ggcgattttt
     2941 caaagctaag taaaattagt attcgtatgt tacgtcatta tgatgaaata ggacttttaa
     3001 ctccttctca tacgaataaa actaatggat atagatacta tagtgcagac caactttcta
     3061 ctacaaacag aatccatgca ctaaaagata tgggctttgg cttgtattct ataaaagaaa
     3121 ttttgacaga atataacgat aaagaaagtc tcataaaata tctaaatatc caccattctc
     3181 aagtaaaaga acaattagaa gatacacaga aaaaactatt aaaaatagaa accactatta
     3241 aaagaattgg aggaaatgat attatgaaaa attatgatgt aactataaag aattttgcac
     3301 caaaatatat gatgacactt agaagggtta taccaactta tcaagacgaa ggaatgttat
     3361 ggcatcaagc atttttagag acaaaagacc agaatgttca aattgaaccc ccaaagtatt
     3421 ctaaagcagt tttttatgat actggatata aagaagatta tgtagatgtt gaagtacaag
     3481 ttgctgtatc tggaaagtat aaagatactg aacatgttaa atttaagact gttccaagtg
     3541 tgactgctgc aactgcaatt gttaatggaa atttcaatca agttgcagac gcttgtgagg
     3601 ctattggtaa ttggatatca gataataact atgatgtgga tggtcctatg tttaatattt
     3661 atcatgtaag tcctggcaac gatagtaatc ctgataattg ggtaacagag gtatgcttcc
     3721 cagttaaaaa gaagtaattt ttatattaat tggataatat tttaatttgt tagacaaatt
     3781 acttttagtg ttatgtataa atacctacaa tttaattagg atattatctt aatttgttaa
     3841 acaaattact tttagtgtta tgtataaata tctacaattt aattaggata ttatcttaat
     3901 ttgtcagata aatcacttgt gatgttatgt ttgaatgact agaatttaat caggatatta
     3961 tcttaatttg tcagataaat cacttgtgat gttatgttta aatgactaga atttgattgg
     4021 gatattatct taatttgtca gataaatcaa ttgtgatgtt gtgtttaaat gactagaatt
     4081 tgattaggat attatcttaa tttgtcagat aaattattta taatgttatg tttaaatacc
     4141 tacaatttaa ttaggatatt atctcaattt gtcagataaa tcaattatga ttttatgttt
     4201 aaatgactag aatttgatta ggatattatc ttaatttgtc agataaatca attataagat
     4261 tatatactat ccttatactc tagttaaata tattttaatc actaatataa attagttttt
     4321 ttaacaaaac aataaaagag taaagtacaa ataattcctt tgtactctac tctttttaac
     4381 tttctttgta caataataca atgataaata taaaaaatta tatattccat tctatagaaa
     4441 tatgctcacc aataacatgt atgttgttaa tcaatatatt atatcaaatt atatcaaaat
     4501 tacataaacc ttataggaca atcatatctt attacaagaa aaatgtctta tccatattga
     4561 ataagtcatt cagttcatat acaaagtcaa tttcattgag aaacatttaa tatatgttta
     4621 taaaaatttc atactattct ttcatattta catgtgatat aatttattaa ctcataattt
     4681 tgttaatcag ttgtgcttgt atattattta tataatctaa tatttttaga aggtggtgta
     4741 ataactttga aattaaaaat tgttggtagt ggcggtatgt caattattcc aagttcattt
     4801 tgtaaatgca aaatatgtga agaagccagg caaaaaggcg gaagatatga acgattagga
     4861 ccaagtttat atattgatga tataaaaatg ctgatagaca cacctgaaga cattgcagta
     4921 gcttgtaaca gacaaaaaat atcggaagta aaacatattt caatttctca ccatgaccca
     4981 gaccatgtta agggtattag aattgtcgaa aaaataggat gtgactttat cacaggagaa
     5041 aataatccaa ttggctttta tgcgcttcct gaagtaattg aagatataaa ccgaacgaat
     5101 ctcgattgtc ttaaatatta tggtgatgtt ataaattgca tttctattaa tgaaacttcg
     5161 catgtaaaga taaataattt tgatattgat ttgattaaca ataacccaga caggaatata
     5221 accttctatg ttatcaaaga gaatgaaaaa aaagtcatat atgcctgctg taatcccaag
     5281 ccatttactc atagtgatat gtactttgat gctgatattt taataataag tctagtttct
     5341 gatgatggta tattaaatga tggtacaaaa cttaaagaca caccatttaa agatgaaata
     5401 tttactttag atgagattgt tgaaattaaa aactattata gaattaaaaa aattattgtt
     5461 acacatattg acgaaatgtg gggaaaatca tatggttatt acagtgaatt agaaaagaaa
     5521 cttgataaca tttattttgc atatgatggt atggaaatta tagtctaaat cattaagtaa
     5581 taagcataat gcaaaataag aaaacttcaa tttaacagta tagtcaaact atgtgatatc
     5641 aacatatcgc agtttttaca aaatatcata gtatttatct ttataaaatt tcaagtccta
     5701 catattttaa tacttcaata tctaaataaa gtaaaatcaa aaatatattt tatatacatt
     5761 atagacggct tatcgtcact agataagccg ttttgtcaca attattattt gctcaacaaa
     5821 acttctcata atatataaca gattgaatct ccaactgata cttaattacc aaaactctta
     5881 ttccttaata tgttgttgca ttaaccaaac cttcatcatc attttttgtg gcaatagttt
     5941 tgctaataaa tgactggatt ttgtatttat accatacaca gaaatatcct tatttttctt
     6001 tgcatccttc aatgcttttt tggcaacaac gtctggagac actatgcctg ggaaattttt
     6061 tgtccctttt tcagctacaa ctatacctct cttaaataga tttgtactca tccaacctgg
     6121 acatacagca attgcattta tccctttact cttaagttca acatttaatg ctcttgtata
     6181 atttcttata aacgctttag ttgaactata aatattttga tatggcaatg gctggaatga
     6241 tgcttgtgaa gaaatattca tgatatggct tcccttttgc atatatggaa tacatataag
     6301 tcccaatgct acaacagcac taatatttaa atcaatcata ttaatagatt catctatact
     6361 taaatcattg tatgaacaga attttgcaaa accagcattg tttataaggt attttataca
     6421 tacattttct ttacttaaaa atgtaccaat ttcttttact tcttctattt ttgataaatc
     6481 ttttgaaaaa atttttacct tacttccaaa ttcatctact aattgattta atctcgcctt
     6541 gtttcttgca actgcccata tttctgttat ttcttcttca tttattaata actttagaaa
     6601 ctctttacca aaaccactat tagcacccgt tataattgca atcttcttat aactcataat
     6661 tattctcctt taccataagt ttatacgctc aaactgtaaa cttatttttt acaaataaaa
     6721 caccctctat tcagatgtta tattcatatt atttgagata ttatatttat atcattttca
     6781 ataattgtta tatagcataa aaaatagtgt taatattata gaatc
//
LOCUS       DAMJVK010000156         6719 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.161, whole genome shotgun sequence.
ACCESSION   DAMJVK010000156 DAMJVK010000000
VERSION     DAMJVK010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6719
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.161"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            365..1639
                     /locus_tag="PC414_003573"
     CDS             365..1639
                     /locus_tag="PC414_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HDF2465924.1"
                     /translation="MQFMDVSKYIEGANLKVKTKSGETVKRIYFNNSATPLVLKNVVD
                     NLNCEIPLLTYINAPGIISEKNTLKYENVRSTILKLIDGDEEKDSVIYVKNATEGINF
                     LAKFFKDENPDKFVITTAMEHMANYLPFKVNFPTKVVGITEKGELDLCQLENILKNYA
                     GNVSLVTVTGASNVTGITTPIYEIARIAHKYGAKILVDIVQVIQHKPFSMMPHECDEH
                     IDFVVFSAHKCYSPLNGGALVGPKKFFEKFNPVLYGSGSTKFVNDNKILYANVPQRFE
                     AGYPEYFGTLAMGKALNTLNKIGLNRISKYERELFLYAKEEMSKIPNIIIYGDKSNNV
                     IIPNISFNISNMYYKDVAKYLVDNFGIETGAGVVGADIYVQKLLGISPKEAYVRFMRE
                     NPVGLVRISLGMYNTFDEVDSFIHAIKTLAFK"
     gene            complement(1846..2163)
                     /gene="trxA"
                     /locus_tag="PC414_003574"
     CDS             complement(1846..2163)
                     /gene="trxA"
                     /locus_tag="PC414_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDF2465925.1"
                     /translation="MVKIVNNNEFINEVENKDGLVVVDFFATWCGPCKMLSPIYEALG
                     NEMVEKANFLKVDIDQSIELAQKFEVSTVPTMLIFKDGKPVDRLIGFMPKENLKNKIE
                     SYM"
     gene            2430..3182
                     /locus_tag="PC414_003575"
     CDS             2430..3182
                     /locus_tag="PC414_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HDF2465926.1"
                     /translation="MDNIINELQNIGFTKYESQIYISLLKESPLTGYEISKLSGVPQS
                     KVYENITKLLNNNIIINVGTDPIKYIPINPNELLKNKKKEFDNSMDNLKNSLQILNKS
                     KSVEYVLNIKGDKNILKKAIEMIKNAKQEIIISCPYEEILELKKELLIAYKKNINIEI
                     LLLENKSLKGLDNIHIHGGNKTTLENKMLYKGIILIVDNDEILTGNLSEGSEAISIWT
                     KNSNILYIGVQYIKHEIYISEQIKGGYKNEQN"
     gene            3169..4050
                     /locus_tag="PC414_003576"
     CDS             3169..4050
                     /locus_tag="PC414_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HDF2465927.1"
                     /translation="MNKIKIKQVDAFTTIPFGGNPAGVVTDTSNISDEIKQNVAKEMN
                     LSETAFVSESNIADFKVQFFTPGLEVDLCGHATIGTFHALLEEGKLNTNKSIFYQETK
                     AGVLSVELKKINNENIFMMEQNTPEFENLDNCRKELAQMIGVHEDRLLEYPIIKVNTG
                     LWWLVFGLKSLDDLKNISPDLNKIKKFSGDNNIIGITPFCIETIDKKCDYHLRSIAPY
                     VGVTEDPVCGTGNACVSSYIVHHNILDKTSFIGEQGNFINRPGKVYVNITRENNNITS
                     VKIGGSAYTVLSGEIMY"
     gene            complement(4276..5631)
                     /locus_tag="PC414_003577"
     CDS             complement(4276..5631)
                     /locus_tag="PC414_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HDF2465928.1"
                     /translation="MSSTVKKKGKFPMGFYICSTTFSFERAAYYASKYLIYIFLTTAI
                     VHGGLGIDKGQAAIMQANLVAFTYLAPIIGGYISDRWIGARYTIPIGMLIMGVGYYLG
                     SIATTVSMVNAMIILVSIGTAFFKGNVSAVNGQLFDSQEELDTAFSVQYSFVNIGSFI
                     GTIAVGILYLKTFAKNGVLGFSQCFFIAAVLCVIGAIWFIYGWRFLGNAGKRPFKEGV
                     VAEKIEEKDKSPLTSMDKKRIWAIILISFFSVIFWVFWYLTYLAVYDYGAAFVNMNVG
                     GFDVPLAWFDSLNSLVCIVLGPVLGALWFKLASRPQGDMSLFKKTGLGLIFLGLAFLM
                     LVGAEFSRGVGAPETAKASILWIIMFGILLSLGEMLFSPLGNSFVSKYAPKKLLGVLM
                     GVWTFATFIAGKGYGYIYAFTLKFDMIKVYTIIPVILFVAAILLFLSDKTLSRLVEDD
                     K"
BASE COUNT         2590 a          953 c          838 g         2338 t
ORIGIN      
        1 cattatgttc taatcaattt ctttactatt tttacaactt tattagtcat attcataatt
       61 atttctaatt agtgtttaac attttatgta aaatttataa aaaaatgtat ataaattaaa
      121 ataagtgact atactaaaaa tatgatacgg ataaacaata agttcaacct atctccccca
      181 aaatcaagtg agctacagtt tcccccaact gtagctcatt ttcgtgtaat ttaataaaca
      241 tctcaacaag aattaattaa caaaacccct tataaaaaca taacatatat taaacaagct
      301 cttgattaaa acttgagctt ttttaataaa aaacagaata gtttattaga taaggggttt
      361 ttttatgcaa ttcatggatg tatctaaata cattgaaggt gcaaacctta aagttaaaac
      421 taaaagtggt gaaactgtta aaagaatata ttttaacaac tctgccactc cattagtttt
      481 aaaaaacgta gtggataact taaactgtga gattcctttg cttacttata ttaatgctcc
      541 aggaataatc tcagaaaaaa atactttaaa atatgaaaat gtacgctcta ctatcctcaa
      601 acttattgat ggagacgaag aaaaggattc tgttatttat gtcaaaaatg ctacagaagg
      661 aataaacttc ttagcaaaat tttttaaaga tgaaaatcca gataaatttg ttattaccac
      721 agcaatggag catatggcta attacctgcc ttttaaagtt aattttccta caaaagttgt
      781 tggaattaca gaaaaaggag aacttgattt atgtcaatta gagaatatac ttaaaaatta
      841 tgctggtaat gtatctctcg ttacagttac aggagctagt aatgtcacag gtataaccac
      901 tcctatatat gaaatagcaa gaatagctca taagtatggt gcaaagatat tggtggatat
      961 tgtgcaagta attcagcata aacctttttc aatgatgcca catgaatgtg atgagcatat
     1021 agattttgtt gtttttagtg ctcataaatg ttattctcca ctaaatggag gagctctggt
     1081 aggacctaaa aaattctttg aaaaatttaa tcctgtacta tatggctcag gttctaccaa
     1141 atttgtaaat gataacaaaa tactatatgc aaacgtaccc caacgttttg aagcaggata
     1201 cccagagtat tttggaactc ttgcaatggg aaaagcactg aacactttaa ataaaattgg
     1261 tctaaacaga atttcaaaat acgaaagaga attattttta tatgctaaag aagaaatgag
     1321 taaaatacct aatataataa tatatggaga taaatcaaat aatgtaatta tcccaaatat
     1381 atcttttaat ataagtaata tgtattacaa agatgtagca aaatatttag ttgataactt
     1441 tggaatagaa actggagctg gagtagtagg tgcagatatt tatgtacaaa aattattagg
     1501 tataagtcca aaagaagcat atgtcagatt tatgagggaa aatcctgtag gtcttgttag
     1561 gataagctta ggaatgtaca atacttttga tgaggtggat agctttatac atgctattaa
     1621 aactttggct tttaaataag ttttttctat tcttttttat caataaatag agagtagttt
     1681 tgttttcaca aaaaagcaaa ttatatcaac agtcaatttc attagcaatt agacactcat
     1741 ttgtaactat acaaactatt ttacactata caaacaaaaa actcattaca atgagttttt
     1801 ttgtttatat atgagattta ttttaattac ttcaatttac tttaattaca tatatgattc
     1861 aattttgttt tttaaattct ccttaggcat aaaacctatt aatctatcta caggttttcc
     1921 atccttaaat attagcatag ttggtactgt agatacttca aatttctgag ctaattctat
     1981 actttggtct atgtccacct tcaagaagtt tgctttttca accatctcat ttcctaaagc
     2041 ttcatatatt ggtgatagca tcttacaagg tccacaccaa gtcgcaaaga aatctacaac
     2101 tactaaacca tctttatttt ctacttcatt tataaattca ttattattta ctatttttac
     2161 cataatatac atctccttta ttatcttctt gatattaata tactacgacc tatataacag
     2221 ttcaattagg cacttttttg taaccgccta tttttaatag atttaatatt atgataaata
     2281 gatttataag attaaatatt tataagacta aatatttcta atatatttta tttttttctt
     2341 aatatagttg acattttttc ccttaaatta taaaatcaaa ttaataacta cataggtagt
     2401 tactaatctt ttatcaaagg agtttctata tggataatat tattaatgaa ttacaaaata
     2461 taggatttac taaatacgaa tctcaaatat acatatcatt attaaaggaa agccctttaa
     2521 caggatatga aataagtaaa ttatcaggtg taccacaatc aaaagtctat gaaaatataa
     2581 caaagctatt aaacaataat ataattatca atgttggaac tgaccctatt aagtacatac
     2641 caataaaccc taatgaatta ttaaaaaata agaaaaaaga atttgacaat tctatggata
     2701 atctaaagaa tagcttacag atattaaata aaagtaaaag tgtagaatac gtattaaaca
     2761 taaaaggaga taaaaatata ttaaaaaaag caatagaaat gataaaaaat gcaaaacaag
     2821 aaattataat ttcttgccct tatgaagaaa ttcttgaatt aaaaaaagaa ctcctaattg
     2881 cttacaaaaa aaatattaat attgaaatac tacttttaga aaacaaaagt ctgaaaggtc
     2941 ttgataatat tcatattcat ggaggtaata aaactacact tgaaaataaa atgctttaca
     3001 aaggaattat tttgattgta gataatgatg aaatattgac aggaaactta tcagaaggca
     3061 gtgaggccat ttcaatttgg acaaaaaact ctaacatttt atatattgga gttcaataca
     3121 taaaacatga aatttatatt tcagaacaaa taaaaggagg ttataaaaat gaacaaaatt
     3181 aagattaaac aagttgatgc ttttacaact ataccttttg gtggaaatcc tgctggtgtc
     3241 gttactgata cctctaacat ttctgatgaa attaagcaga atgtggctaa agaaatgaat
     3301 ttatcagaaa ctgcatttgt ttcagagtca aatattgcag attttaaagt acaatttttc
     3361 acaccaggtt tagaagttga tttatgtggt catgcaacaa taggtacttt ccatgcactt
     3421 ttggaagaag ggaagcttaa tactaataaa agtatatttt atcaagaaac aaaagctggt
     3481 gtattatctg tagagcttaa gaaaataaat aatgaaaata tatttatgat ggaacagaat
     3541 acacctgaat ttgaaaattt agataattgc agaaaagaac ttgcacaaat gataggtgtg
     3601 catgaagata gacttcttga atatcctatt ataaaggtaa atactggact ttggtggctt
     3661 gtatttggtc taaaatcttt agatgattta aaaaatatct ctccagattt aaataaaatc
     3721 aaaaaattta gtggtgataa taatataatt ggtataactc cattttgtat agaaactata
     3781 gataaaaagt gtgactacca tttaagatct atagcaccat atgttggagt tacagaagac
     3841 cctgtatgtg gaactgggaa cgcttgcgta tcatcttata tagtacatca caatatttta
     3901 gataaaacta gttttattgg tgagcaaggt aactttataa acagacctgg aaaggtatat
     3961 gtaaatataa ctagagaaaa caataacata acttctgtaa aaattggagg aagtgcttat
     4021 actgtcttaa gtggtgaaat tatgtattaa tcccatacct aataaaaaca aataaaaata
     4081 ctagctaact aaagctagta tttttatttg ttatttttat ttaataatca tactctaaca
     4141 aatattataa ttatatatat cataatattg tatttagaag taaaatttta tttttattat
     4201 taattagtta taaatcaatc taatacaaca taattacagt agattaacct ataaaacata
     4261 ataattgctt actgattatt tatcgtcctc aacaagtcta cttaatgtct tatcagataa
     4321 gaataataat atcgctgcaa caaatagtat tactggaatt atagtatata ctttaatcat
     4381 atcaaattta agagtaaatg catatatata tccatatcct ttaccagcta taaaagtagc
     4441 aaatgtccat actcccataa gaacacctag aagcttctta ggtgcatact tacttacaaa
     4501 agagttacca agtggtgaga ataacatttc accaagactt aaaagaatac caaacatgat
     4561 tatccatagt atactagctt tagcagtttc aggagctcca actcctcttg aaaattctgc
     4621 accaacaagc attaagaatg caagacctaa gaatattaat ccaagacctg tctttttaaa
     4681 taaactcata tctccttgtg gtctactagc aagtttaaac cataatgctc caagaactgg
     4741 tcctaatact atacatacaa gtgaatttaa tgagtcaaac caagcaagag gaacatcaaa
     4801 tcctccaaca ttcatgttta caaatgctgc accataatca taaactgcaa gataagttag
     4861 ataccagaat acccagaaaa ttacagagaa gaatgatata agtataatag cccaaattct
     4921 cttcttatcc atgcttgtaa gaggagactt atctttttct tcaatttttt cagcaactac
     4981 accttcttta aaaggtctct ttcctgcatt cccaaggaat ctccatccat atatgaacca
     5041 tattgcacct attacacaaa gtacagctgc tataaagaaa cattgagaaa atccaagtac
     5101 accatttttt gcaaatgttt taaggtaaag aataccaact gctatagtac caataaaaga
     5161 acctatatta acaaaagaat attgtactga aaatgctgta tcaagttctt cttgactatc
     5221 aaaaagctgt ccatttaccg cagatacgtt acctttaaag aatgcagtac caattgaaac
     5281 taagattatc attgcattta ccatagaaac tgtagttgca atagaaccaa gatagtatcc
     5341 tacccccatg ataagcatac ctataggtat agtatatcta gctcctatcc atctatctga
     5401 tatatacccc ccaattatag gtgcaaggta tgtgaatgca acaaggttgg cttgcattat
     5461 agcagcttgt cctttatcta ttccaagacc accatgaact atagctgttg taaggaatat
     5521 atatattaag tactttgaag cataatatgc tgctctttca aatgagaaag ttgttgagca
     5581 gatataaaat cccataggaa atttaccctt cttttttact gttgagctca taattttaaa
     5641 tcctttctta attgtatttt tattttttac gttttgtcca aattctttct tttatgaaag
     5701 atataatatt attattttgt aaaacaattt gtaattatta ttaagataat tgcgcattta
     5761 tttgtagagt acatattctc atggaataaa attaatacac ttcttaatca taaatttaaa
     5821 ttatataaat tcactatttt cagatatagt tataaattaa tatattctca tctaatacca
     5881 tgaaaaaacc ttcgccatat gcaattctta ttttttacgt gatagtattt tacaaactaa
     5941 aatccccctt ttgtagcaac ttatgtaatt acattttagt gcttttttaa aggagtctcg
     6001 aataaagcaa catatatttt gttcaaataa tgtttttatt tattttttaa atttatatat
     6061 tacttcaccc aaccaattct caacttaaat aactaaacta ctatctattc acagattagg
     6121 cttatttctt acacattagt ttataataaa cactgtgaat ctaaacataa aaactgttat
     6181 tttaaagtaa ttacccatat attcgctttg atactattta ttgaggctat actctttatg
     6241 tataatatat tatttataaa atacctcaaa atatattatt cataaaatac ctcaaactga
     6301 aaactttttc acgaatattt atttttcacc tccccttttt tatttcacca tgtaaatcgt
     6361 accataaata tgcacttata tgcaagaaaa aaatctatta atcgacagta aataattaga
     6421 aaaattcaaa ttatatttat caattatatt atataaaata tttcctatga aaatataaat
     6481 taaattaatg ttcagaatag tttttagata tgaaatcttg atttttttat tactttactt
     6541 atttgctcaa atatattttt gtatgcagta tataataatc aaattataca cttcttataa
     6601 ctgcattgat ttgaaaataa tattaataat taatagaata aaaaatttaa attaaattta
     6661 agaaaatttg ttaagttgat tgaatacttt tttatgtatt taattaattt tataaattc
//
LOCUS       DAMJVK010000157         6388 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.162, whole genome shotgun sequence.
ACCESSION   DAMJVK010000157 DAMJVK010000000
VERSION     DAMJVK010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6388
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.162"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            302..1246
                     /locus_tag="PC414_003578"
     CDS             302..1246
                     /locus_tag="PC414_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HDF2465929.1"
                     /translation="MKFLRIEDEVIYSKKRYKLKREIDKLFLDEEYFQIEPQLFEEYD
                     EFTTINNKIPEESMVKVVNGKVMVLRADITTSIIKSLVPRWEDGLKLKLFYNSSIYKN
                     KNTVGIKEIRQIGCEYLGEASVEADREVVKLALKILEKYNNNFILEVGSSKYIHGLLE
                     ELNLNKNCENQIKNLLYTKNTHELKVYIEELKIKREVKELLSNILSLQGNLYNVIEKS
                     NKFYCNNKMKQALEELKQVNNLIEECNFLDKARFDLSMITMLDYYEGIMFRGYYPNSY
                     KEILSGGRYDSLTKEYGKEIPAIGFTLSVDELMKYVYK"
     gene            1268..1894
                     /locus_tag="PC414_003579"
     CDS             1268..1894
                     /locus_tag="PC414_003579"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HDF2465930.1"
                     /translation="MDYLTIALAKGRIEGESFKKFKKMGLGDSIDTDTRKLIFKDEEN
                     KIIYIHVKPSDVVTYVEKGVADLGIAGKDTILENETDVYEIYDLGFGKCKFAVAGLKG
                     DSIYREDEYLKVATKYPNIAKKYFKEKGQKIEIIKLNGSVELAPIVGLSDVIVDIVET
                     GNTLKANGLEILEDICNISARIISNRASYRFKYEQIQNIIRLFEELDN"
     gene            1928..2977
                     /gene="hisC"
                     /locus_tag="PC414_003580"
     CDS             1928..2977
                     /gene="hisC"
                     /locus_tag="PC414_003580"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HDF2465931.1"
                     /translation="MREKESIRELRGYEPNHVNCKVKLDANEGSKRLFKYLIKEISDS
                     DIDLNLYPEDSYSDLKESIIDYINISGVNKKNLLVGNGSSEIIDLIIHTFVDKDEVIL
                     SFSPSFSMYSIYSQINGSKFIGVESDENLVINIDNVIEKVKENNPKIVIVCNPNNPTG
                     TILKREEIIKLLDSTNSLVVLDEAYMDFGEESMLSDVFKYDNLIVLRTLSKAFGLAGI
                     RTGYMLSNSSLINSVEKVRPPYNLNSLSDFIATRALRNKDVVKAYIKEVKEEREVLYE
                     EMIGMGIKAYKSQANFILFYSEIENLSQKLIDRGVLIRKFGGKLENYYRVTIGDKEEN
                     SMFVGAIRDILKKEK"
     gene            2980..3558
                     /gene="hisB"
                     /locus_tag="PC414_003581"
     CDS             2980..3558
                     /gene="hisB"
                     /locus_tag="PC414_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HDF2465932.1"
                     /translation="MRIWKVERNTLETQILVELNIDGSGKAEIDTGIGFLDHMLTLMS
                     FHGKFDLKVICKGDTYVDDHHSVEDIGIAIGEAFKNALGDKKGIRRYSNIYIPMDESL
                     SMVAIDISNRPYLVFNAKFDTQMIGSMSTQCFKEFFRAFVNESRVTLHINLLYGENDH
                     HKIESIFKAFARALKEGSEIVSNEIASSKGVL"
     gene            3560..4165
                     /gene="hisH"
                     /locus_tag="PC414_003582"
     CDS             3560..4165
                     /gene="hisH"
                     /locus_tag="PC414_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HDF2465933.1"
                     /translation="MNIIVDYGLGNIDSVSRGFRKAGIETKISSDIDEIKQADSLILP
                     GVGAFRDSISALDKLGLIPIIKEHVSKGKFMIGICLGMQLLYEKSYEYGEYEGLGLIK
                     GSIDKLDISLKVPHMGWNNLKFNKANDDILKYINEDDYVYFVHSYYANSSNEELIAFS
                     EYEKKIPAIVRKGNVYGIQFHPEKSGEVGLNILRAYGEMIK"
     gene            4162..4884
                     /gene="hisA"
                     /locus_tag="PC414_003583"
     CDS             4162..4884
                     /gene="hisA"
                     /locus_tag="PC414_003583"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HDF2465934.1"
                     /translation="MIIFPAIDIKDNKCVRLTQGEFDKVNVYYDNPLEVAYKWKNEGA
                     EYIHIVDLNGARSEFGVNTKIIEDIANNIDIPIQVGGGVRDKEKVKSLINAGVTRVIL
                     GSIAIENLNLVEELVNEYKEKIVVSIDAKDGKVAVRGWEVVSNVDSLTLCKQLEKIGV
                     QTIVYTDISKDGMLQGPNFDIYERIAKETSLNVIASGGVTSIEDVKRLKAMNLYGAII
                     GKALYDKKIDFKEAQQLCLLGE"
     gene            4866..5624
                     /gene="hisF"
                     /locus_tag="PC414_003584"
     CDS             4866..5624
                     /gene="hisF"
                     /locus_tag="PC414_003584"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HDF2465935.1"
                     /translation="MLTRRIIPCLDVRNGRVVKGKKFKDIVDVDSPEVLGKFYSDCGA
                     DELVFYDITASNEERKTSLEFVTKVAENINIPFCVGGGVNKLEDFTDILRKGADKVSI
                     NSSAVKNPELIREASLKFGAQCVVLSIDAKKNEEGSWSVYVKGGREKTNLDAIEWAVK
                     GVELGAGEIVVNSMDEDGMKNGYDIELLSKITSLVNVPVIASGGAGKKEDFYEAVNKS
                     NVDGILAASVFHFGEIKINDLKKYLKDMGVEVRL"
     gene            5652..6320
                     /locus_tag="PC414_003585"
     CDS             5652..6320
                     /locus_tag="PC414_003585"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HDF2465936.1"
                     /translation="MDNKCNNVYSDEVEKFIRSIKFDDKGLVPVVVQEVVSKDVLMLA
                     YMNKDVIKKTLKDKVACYFSRSRQELWVKGETSGNTQKVVKMSYDCDVDTILLFVEQT
                     GVACHTGNYSCFYRDLFDDSSKMELEVQTNILKELYDLINERKNNPVEGSYTNYLFEK
                     GIDKILKKVGEESSEVIIASKNTDKSELIYEISDLVYHTLVLMIEKGVEIDEIKKELL
                     KRRK"
BASE COUNT         2612 a          539 c         1260 g         1977 t
ORIGIN      
        1 caaaaaactg taaatttaga atattaataa aaattatttg ggaggaaatt atcatggtga
       61 gattaaatat agtaaaatta aatacaaaat taaatcatca ccatcatcat agattaaagg
      121 gattgtaata tgaaattaat tcatagacac aagccctgtt tttagtgtaa ttggtcttgt
      181 atctatgatg ggaagtaatt ttaagagaaa attatagcca tataggtata aagcctatgt
      241 ggctttttta ttgttttaaa tttgcaaata taagagtaaa tttaatgaag agggtgagaa
      301 tgtgaaattt ttgaggattg aagatgaggt tatttattct aaaaaaagat ataaattaaa
      361 aagggaaata gataaacttt ttttggatga agaatatttt caaatagaac cacaattatt
      421 tgaagaatat gatgaattta caactataaa taataaaatt ccagaagaat ctatggtaaa
      481 ggttgtaaat ggaaaagtaa tggtacttag agctgatata actacaagta taataaaaag
      541 ccttgtacca agatgggaag atggtttaaa gcttaagctt ttttataatt catcaatata
      601 taaaaataaa aatacagttg gaataaaaga gataagacaa ataggttgtg agtatctagg
      661 agaagcctct gtagaagcag atagagaagt agtgaaatta gctcttaaga tacttgaaaa
      721 atacaataat aattttattc tagaggttgg gagtagcaag tatattcatg gtttgttaga
      781 agaattaaat ttaaataaga attgtgaaaa tcaaattaaa aatttactat atacgaagaa
      841 cactcatgaa ctaaaagtat atattgaaga attaaaaatt aagcgtgaag taaaagagtt
      901 attatcaaat attttaagtt tacaagggaa tttatataat gttattgaaa aatcaaacaa
      961 gttctattgt aataataaga tgaagcaagc tcttgaagaa ttaaagcaag taaataattt
     1021 aattgaggaa tgtaattttt tagataaggc tagatttgat ttatcaatga ttactatgct
     1081 tgactattat gaaggaatta tgtttagagg atactaccca aactcatata aagaaattct
     1141 tagtggcgga agatatgatt ctttaactaa agagtatgga aaagaaattc cagccattgg
     1201 atttacacta agtgttgatg agttaatgaa gtatgtatat aagtaaagtt tagaaggagg
     1261 gattgctttg gattatttga ctatagcact agcaaaagga cgaattgaag gggaatcatt
     1321 taaaaaattt aaaaaaatgg gtctaggaga cagtatagac acagatacga gaaaactaat
     1381 ttttaaagat gaagaaaata aaattatata tatacatgta aagccatcag atgtagttac
     1441 atatgttgaa aaaggagtcg ctgacctcgg tatagcaggt aaagatacta ttttagaaaa
     1501 tgagacagat gtatatgaaa tatatgattt aggttttgga aaatgtaaat ttgctgtagc
     1561 aggattaaag ggagatagca tatatagaga agatgaatat ttaaaagtgg caacaaagta
     1621 tcccaatata gcaaaaaagt attttaaaga gaaaggtcaa aaaattgaaa taataaaatt
     1681 aaatggttct gtagaactag ctccaatagt agggttgtca gatgtaattg tagatatagt
     1741 tgagactggc aatacactta aagcaaatgg tcttgaaata ttagaagaca tatgtaatat
     1801 aagtgctaga attatatcaa atagagctag ctatagattt aaatatgaac aaattcagaa
     1861 tataattcgt ttatttgaag aacttgataa ttaaaatata attgagcttt ataaggaggg
     1921 atttaagttg agagaaaaag aaagcataag agagttaagg ggatatgaac caaatcacgt
     1981 aaactgtaaa gtaaaacttg atgcaaatga aggaagtaag aggttgttta aataccttat
     2041 taaagaaatt tcagatagtg atatagattt aaatttatat cctgaagatt cttatagtga
     2101 tttgaaagaa tctataatag attacataaa tatttctgga gtaaataaga aaaatcttct
     2161 agtaggaaac ggttcaagtg aaataattga tttaataata catacttttg tagataaaga
     2221 tgaagtaata ttgagtttct ctccaagttt tagtatgtat tctatatata gtcagataaa
     2281 tgggtctaaa tttataggtg ttgaaagtga tgaaaattta gttataaata tagataatgt
     2341 gattgaaaaa gtaaaggaga ataatcctaa aatagtaatt gtatgtaatc ctaataatcc
     2401 aactggaaca atattaaaga gggaagagat aataaagctt ttagattcaa ctaatagtct
     2461 agtggtttta gatgaagcat atatggattt tggagaagaa agcatgttaa gtgatgtttt
     2521 taagtatgat aatctaatag ttcttagaac tttatcaaag gcatttggat tggctggaat
     2581 aagaacagga tatatgttat ctaatagtag tttaataaat tctgttgaga aagtaagacc
     2641 tccatataat ttaaattcat tatcagactt tattgctaca agagcactta gaaataaaga
     2701 tgtggtaaag gcttatataa aggaagtaaa agaggaaaga gaagttttat atgaagaaat
     2761 gattggtatg ggaattaaag catacaaatc acaagcaaat tttatattat tttattctga
     2821 aatcgaaaat ttatcacaaa aacttattga tagaggtgtt ttaataagaa aatttggtgg
     2881 aaagttagaa aattattaca gagttacaat tggtgataaa gaagaaaatt ctatgtttgt
     2941 tggagctata agagatattt tgaaaaagga gaaataggta tgagaatttg gaaggtagaa
     3001 agaaatactc ttgaaacaca gattttagta gaattaaata ttgatggttc tggtaaagct
     3061 gaaatagata ctggaatagg atttttagac catatgctta ccttaatgtc atttcatggc
     3121 aagttcgatt taaaagtgat ttgtaagggt gatacatatg tagatgacca ccatagtgta
     3181 gaagatatag gtatagctat aggtgaagca tttaaaaatg cactaggtga caaaaaagga
     3241 atacgaagat attctaatat ctatatacct atggatgaat ctctatcaat ggtagcaata
     3301 gatataagca atagacctta tcttgtattt aatgctaaat ttgataccca aatgattggt
     3361 agtatgagta ctcaatgctt taaagaattt tttagagctt ttgtaaatga atcgagagta
     3421 acattacata tcaatctctt atatggggaa aatgaccatc ataagataga aagtattttt
     3481 aaagcatttg caagagcttt aaaagagggt agtgaaatag tttctaatga gatagcatct
     3541 tcaaaggggg ttttgtagaa tgaatataat agtagactat ggtcttggaa atatagattc
     3601 tgtgtctagg ggttttagaa aagctggaat agaaactaaa atttctagtg atatagatga
     3661 aataaaacag gcagattcac ttatacttcc aggtgttgga gcttttagag attctataag
     3721 tgccttagat aaactaggat tgataccaat aataaaggag catgtttcta agggtaaatt
     3781 tatgataggt atatgtctag gaatgcaatt actttatgaa aaaagttatg agtatggaga
     3841 atatgaagga ttaggactta taaaaggaag tattgataaa ctggatataa gtttaaaagt
     3901 gccacatatg ggatggaata atctaaaatt taataaggca aatgatgata tacttaaata
     3961 tataaatgaa gatgattatg tatacttcgt tcactcatat tatgcaaatt catcaaatga
     4021 agaactaatt gcattttcag aatatgaaaa gaaaatccct gctattgtta gaaaaggcaa
     4081 tgtatatggt atacaatttc atcctgaaaa aagtggagaa gtagggttaa atatattaag
     4141 agcatatggg gagatgataa aatgataata tttccagcaa tagatataaa agataataag
     4201 tgtgtaagac tcacacaagg agaatttgac aaagtaaatg tatattatga taatccatta
     4261 gaagtagcat ataaatggaa aaatgaagga gcagaatata tacatatagt agatttaaat
     4321 ggtgctagaa gtgaatttgg tgtaaataca aaaataattg aggacatagc aaacaatata
     4381 gatataccta ttcaagttgg cggtggagtt agggataagg aaaaagtcaa aagtttaata
     4441 aatgctggtg ttactagagt aatattaggt agtatagcga ttgaaaattt gaatttagtt
     4501 gaagaattgg tcaatgaata taaagagaaa attgtagttt ctatagatgc taaagatgga
     4561 aaagtagcag ttaggggatg ggaagttgta agcaatgtag actcattaac attatgtaaa
     4621 caacttgaaa agataggtgt acaaactatt gtttatacag atatatcaaa agatgggatg
     4681 ttgcaaggtc caaattttga catatatgaa agaatagcaa aggaaacatc actaaatgtt
     4741 attgcttctg gaggcgtaac ttctatagaa gacgtaaaaa gactaaaagc tatgaactta
     4801 tatggtgcaa taataggtaa agcgctttat gataaaaaga tagactttaa ggaggcacaa
     4861 caattatgct tactaggaga ataatacctt gtctagatgt gagaaatgga agagttgtaa
     4921 aggggaaaaa atttaaagat atagtagatg ttgatagtcc agaagtactt ggaaaatttt
     4981 atagtgattg tggtgcagat gaacttgtat tctatgatat aactgcatca aatgaagaga
     5041 gaaagacatc tttagaattt gtgacaaaag ttgcagaaaa tataaatata ccattttgtg
     5101 ttggtggagg agtaaataaa ttagaagatt tcactgatat tttaagaaaa ggtgctgata
     5161 aagtttctat aaattcatca gcagtcaaaa atcctgaatt aataagggaa gcttcattaa
     5221 aatttggagc acaatgtgtt gttttatcga tagatgcaaa gaaaaatgaa gagggttcat
     5281 ggagcgtata cgtaaaaggt ggaagagaaa aaactaatct ggatgcaatt gaatgggctg
     5341 ttaaaggtgt agaacttgga gcaggagaaa tcgttgtaaa tagtatggat gaagatggaa
     5401 tgaaaaatgg atatgatata gaattattat caaagataac ttctttagta aatgtacctg
     5461 ttatagcatc tggaggcgct ggaaaaaagg aagattttta tgaggcggtt aataaatcca
     5521 atgtagatgg aattttagca gcatctgttt ttcattttgg cgagataaaa ataaacgatt
     5581 taaagaaata tttaaaagac atgggtgtag aagtaagatt ataagttaaa tactaaaagg
     5641 agattagaaa tatggataat aagtgtaaca atgtatacag tgatgaagta gagaaattta
     5701 taagaagtat aaagtttgat gataaagggt tagtccctgt tgttgttcaa gaggtagtta
     5761 gcaaagatgt actgatgtta gcatatatga ataaagatgt gataaagaaa accttaaaag
     5821 ataaagtagc ttgctatttt agtagaagta gacaagaact ttgggttaaa ggtgagacat
     5881 ctggtaatac tcaaaaagta gttaagatgt cttatgattg tgatgtagat actatactac
     5941 tttttgttga acaaactggt gtagcttgtc atacaggcaa ttattcttgt ttttacagag
     6001 acttgtttga tgatagttca aaaatggagc ttgaagtaca aacaaatata ttgaaagaat
     6061 tatatgattt aatcaatgaa agaaaaaata atccagttga aggttcttat actaattatc
     6121 tttttgaaaa aggaatagat aagattctta aaaaagtagg tgaagagagt agtgaggtaa
     6181 ttatagcatc aaaaaatact gataaaagtg aacttattta cgaaattagc gatttagtat
     6241 atcatacatt ggttttaatg atagaaaaag gtgttgaaat tgatgaaata aaaaaagaat
     6301 tacttaaaag aagaaaatag aataagatag attaatgggt aataagaaaa tataccactt
     6361 ttaataaaaa gtggtatatt ttttattt
//
LOCUS       DAMJVK010000158         6258 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.163, whole genome shotgun sequence.
ACCESSION   DAMJVK010000158 DAMJVK010000000
VERSION     DAMJVK010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6258
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.163"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            221..1585
                     /locus_tag="PC414_003586"
     CDS             221..1585
                     /locus_tag="PC414_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HDF2465937.1"
                     /translation="MDIKELVKMQRKYYNTGKTRDISFRIEQLKKLKLVVSQNEEKIL
                     LALKKDLNKSDFEGFMTEVGMFYSELNFAIKNIRKWSKIKRVKSSMVNFPSISKVVPQ
                     PYGVTLIMSPWNYPFQLALIPLVWSLAAGNCVILKPSEYSVSTSSVVKDIVEDTFSKE
                     YVAVVQGSQEESEKLLLERFDYIFFTGSTNVGKIIMKSASEHLTPITLELGGKSPCII
                     LKDANIDLTAKRLTWGKLINAGQTCVAPDYVLVHEDRKNELIEKIKYYTNKYFGDNAC
                     NNEQFPKIINQRHFNRILSLIDKDKIVYGGNYNKETLKIEPTIVDNVNWDDNIMKEEI
                     FGPIFPILTYKDLDEVIQKIIQMPNPLALYIFTKNKYLENKLLEMIPAGGCCINDTVT
                     HIATNYLPFGGIGESGMGSYHGKAGFDTFTHYKSVLKKLNLDVPIRYAPYDNKLIKVL
                     KKIM"
     gene            1724..2890
                     /locus_tag="PC414_003587"
     CDS             1724..2890
                     /locus_tag="PC414_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDF2465938.1"
                     /translation="MKINWNTKYTTIAIYTFIIAASSIIFYLVSSQIDVFSNNLDAIF
                     TTLQPFIIGFAIAYLLNFILKFYEDRIFIKSEKLKKLKQSSKRGLGLLLTYATATLIL
                     YLFMHFVLPQVIESIVGLANDIPMYVNNATKLIDKLMTDLNLDEQYFNLAVDKWNEFV
                     TYIIKFVTDLIPILGSMLKNVASSIWNIVLGLIVSVYLLIDKEKFYGLSKKITYAVFT
                     EKQAARILELTHRSNYTFGRFLGGKILDSFIIGILTFVILTLVKMPYTLLISVIIGIT
                     NIIPFFGPLFGAIPSTLIVMFVSPIKAFWLLLIILIIQQIDGNIIGPKILGDSIGISA
                     FWILFSLLVAGKLLGFIGMVIGVPMFAVIYSIIKDIVESKLDKKGLPTDTSDYM"
     gene            3007..3789
                     /locus_tag="PC414_003588"
     CDS             3007..3789
                     /locus_tag="PC414_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDF2465939.1"
                     /translation="MAFIECKFKSKYTGGETDIIVILPVANGKELFSGKEIYDFEGKF
                     KTLYLFHGLEDDQSSWIRYTNVEHYAKNKNIALVIPRVETSFYTDMAYGHQYFTFVSE
                     ELPKFVRAIFPLSDKREDNFVAGMSMGGYGALKLALSKPKEFSAVASFSGSPDMIHEL
                     ENQQIETNADILFHDFGRPEDARNSENDLLYILKNLKERNEDIPKIYQFCGDRDFLYK
                     NNQMFKKYADSLGVDVEYEECVGGHEWRLWNLWIKKFIDIIG"
     gene            complement(3908..4561)
                     /locus_tag="PC414_003589"
     CDS             complement(3908..4561)
                     /locus_tag="PC414_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2465940.1"
                     /translation="MSKSEKGKETKASIISAARELFFEQGYHKTTTRQIAERANINLG
                     LISYYFSSKSEIGAIIYEDIRNEMQSLIYNYHEEGTMMMFFLAMNADLCFLLKNEAYR
                     NFYLDIAYEEAILSYQRSVTEMVMKRYVNDKESIEHDELILSCIGILAMKPEIIRVYT
                     SHKYDISSQAVIKHFTKQFLINLGHSTDLCEEILEELSKYYIDIVDNFTPIITRVRT"
     gene            complement(4585..5949)
                     /locus_tag="PC414_003590"
     CDS             complement(4585..5949)
                     /locus_tag="PC414_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HDF2465941.1"
                     /translation="MLLLIVTCAIYIWIGFYFSKKVKTASDFLIAGRNLPLPILAATI
                     AATSFGGGCLVGGVQWGAERGLWVGMYSTIGAALACFINAFFAGKLRSLSCDITPADY
                     IETRYGHSVFLRAYQSLVTPISILAIMGSQLISFGSVSTAFGIPYDIAVIIGVIVIII
                     YTYTSGMWGVAVSAFIQLAICIVFLPIVAYISLKLVGDNPLLMLQSMVKEPFFPGNKS
                     ISDFMYTVLPLVLGSIFSYESFLRYQCADNAKNAVKSSVIAGFILLFLAFPIGIIGAI
                     GGSLFPNISSVQVLPQMISTTLPPLISVLFLAAILAAIMSTADSLMTSMSAIISRDIY
                     NKLLHPNIEFNDLKNTLKYSQIASALTAIIGAIIALKFDSLLELLFWPAPLCTGVIFA
                     PFVIGLCWKGATKKGAIYAVIVSVILALLNMIGILTVFDRILVPIIGGSITIFIVSKF
                     SKKD"
BASE COUNT         2359 a          770 c          944 g         2185 t
ORIGIN      
        1 ataatttaat tgtttcaata aggttataat tttaaaaaat gtttatttta aaataaacta
       61 ttttataagc ttacttatac ttaataaggg gcttttttta ttggaagatt tatgttatat
      121 tgagatgaat ttattatgat ataaaaaata atgttaggat aaatttaaat ctttttctaa
      181 aaatgtatta gtataattct taaagaaagg atttttaaca atggatataa aagagttagt
      241 taaaatgcaa agaaaatatt ataatacagg taaaacaaga gatatatcgt ttcgtataga
      301 acagttgaag aaacttaagt tagttgtatc acaaaatgaa gaaaaaatac ttttagcact
      361 aaagaaagat ttaaataaat cggattttga aggttttatg acagaggtag gcatgtttta
      421 tagtgaatta aactttgcta taaagaatat aagaaaatgg agcaaaataa aaagagttaa
      481 atcaagtatg gttaattttc catctatatc caaagttgtt ccacaacctt atggtgttac
      541 tctcataatg tcaccttgga attatccatt tcaattagca cttataccat tagtttggtc
      601 attagcagca gggaattgtg ttatattaaa accttctgaa tattctgtgt caacatctag
      661 tgtagttaaa gacatagttg aagatacttt ttcaaaagaa tatgttgcag ttgttcaagg
      721 aagtcaagaa gaaagtgaaa agttactatt ggaaagattt gattatatat tttttacagg
      781 aagtactaat gttggtaaaa taatcatgaa atcagcatca gagcatttaa ctcctattac
      841 tttagaactt ggaggaaaaa gtccttgtat cattttaaaa gatgctaata tagaccttac
      901 tgccaaaaga cttacttggg gaaaactaat aaatgcagga caaacctgcg tggctccaga
      961 ttatgtttta gtgcatgaag atagaaaaaa tgagttaata gagaaaataa aatattatac
     1021 taataagtat tttggggata atgcatgtaa taatgagcaa tttcctaaaa tcataaatca
     1081 gagacatttt aatcggatat tatctcttat tgataaagat aagatagtct atggtggaaa
     1141 ttataataag gagacattaa agatagagcc aactatagta gacaatgtta attgggatga
     1201 taatataatg aaggaagaaa tatttgggcc tatatttccc attttaacat ataaagattt
     1261 agatgaagta atacaaaaaa ttatacaaat gccaaatcca ttggcacttt atatttttac
     1321 aaaaaataag tacctagaaa ataaactttt agaaatgatt ccagcaggcg gatgttgtat
     1381 caatgataca gttactcata tagctacgaa ttacttacct tttggtggta ttggtgaaag
     1441 tggtatgggc tcatatcatg gaaaagctgg attcgataca tttactcact ataagagtgt
     1501 gttaaaaaaa ttaaatttgg atgtccctat tagatatgct ccatacgata ataaattaat
     1561 taaggttctt aaaaagataa tgtaggttat attgtagaat aataaatata aataaaattg
     1621 agtatttgta ttctgaatat attttatttt taatttggta tatttgttat aaaagtggtg
     1681 attttgtgat aaaatttaat tatatagaaa taaggaggaa aaaatgaaaa ttaattggaa
     1741 tacaaaatac acaacaatag caatttatac atttataata gctgctagta gtattatatt
     1801 ttatctagta tcatcacaaa ttgatgtatt ttctaataat ttagatgcta tatttacaac
     1861 attacaacct tttataatag gatttgctat tgcatatctt cttaatttta ttttaaagtt
     1921 ttatgaagac agaattttta taaaaagtga gaaattaaaa aaactaaaac aaagttcaaa
     1981 aagaggcttg ggtcttttat taacatatgc aacagctaca ttgatacttt acttatttat
     2041 gcatttcgta ttgccacaag taattgagag tattgttgga ctagcaaatg atatacctat
     2101 gtatgtaaac aatgcaacta agcttattga taaattaatg actgatttaa atcttgatga
     2161 gcaatacttt aatttggcag ttgataagtg gaatgaattt gttacatata ttataaagtt
     2221 tgtaacagat ttaatcccaa tattaggaag tatgttaaag aatgtagcct ctagtatatg
     2281 gaatattgta ctaggattaa ttgtttcagt gtacttatta attgataaag aaaaattcta
     2341 tggattaagc aaaaaaataa cttatgcagt atttacagaa aaacaagctg ccagaatttt
     2401 agaattaact catagaagta actatacatt tggaagattt ttaggtggca aaatattaga
     2461 ttcattcatt ataggtattc taacatttgt aatacttacg ttagttaaaa tgccatatac
     2521 attattaata tcagttataa ttggtattac aaatattatc cctttctttg gacctttatt
     2581 tggagctata ccatctacgc taatagtgat gtttgtatct cctataaaag ctttctggtt
     2641 attgttgatt atattaataa tacaacagat agatggaaat attataggac ctaaaatact
     2701 tggagattca attggtatat ctgcattttg gattttgttc tccttattgg ttgcaggaaa
     2761 attattaggt tttattggaa tggttatagg agttccaatg tttgcagtaa tttattctat
     2821 aattaaggat attgttgaaa gtaaattgga caaaaaggga ttaccgacag atacttccga
     2881 ttatatgtag ctaaaaaata ttatgtataa ttaaaaatat ataaattaag caatatttaa
     2941 agcactgaga aagttaaact cttggtgctt tttagatata aaattattat agaagaggtg
     3001 ttttttgtgg catttataga atgtaaattt aaatctaaat atactggagg agaaactgat
     3061 ataattgtaa ttttgcctgt agcaaatgga aaagaacttt ttagtggtaa agagatatat
     3121 gattttgaag gtaaatttaa aacattatac ttatttcatg gtttagaaga tgaccaaagc
     3181 tcatggatta gatatactaa tgtagagcat tatgctaaaa ataaaaatat agccctagta
     3241 ataccaagag tagaaactag tttttatact gatatggcgt atggacacca atattttact
     3301 tttgtaagcg aggaattacc taaatttgtt agagctatat ttcctttatc agataaaagg
     3361 gaagataact ttgtagctgg tatgtctatg gggggatatg gagctttaaa attagcgctt
     3421 tcaaaaccaa aagagttttc ggcagttgca agtttttctg gttcgcctga tatgattcat
     3481 gagttagaaa atcaacaaat agaaaccaat gcagacattc tatttcacga ttttggtaga
     3541 ccagaagacg ctagaaatag tgagaatgat ttactttaca tacttaagaa ccttaaagaa
     3601 agaaatgaag atattcctaa aatttatcaa ttttgtggtg atagagattt tttatataaa
     3661 aataatcaga tgtttaaaaa atatgcagat tctctgggtg tagatgttga atatgaagag
     3721 tgtgtagggg ggcatgaatg gcgtctttgg aatttatgga taaagaaatt tatagatata
     3781 attggatagt acacttatgg tattaacagg tatactataa aatatcttta aatatgataa
     3841 agactgaaat cctaaaatca aattatgatt ttaaaatttc agtctctaag aatttaataa
     3901 aattttatta agttcttaca cgagttatta ttggagtaaa attgtcaact atatctatat
     3961 agtatttact caattcctct aaaatctctt cgcataaatc cgtactatga ccaagattaa
     4021 taagaaactg ttttgtaaaa tgctttatta cagcttgaga agatatatcg tacttgtgtg
     4081 aagtataaac tctaattatt tcaggtttca tagcaagtat accaatacaa gaaagtatta
     4141 attcatcatg ttctatagat tctttatcat taacatatct tttcatgacc atttcggtta
     4201 cacttctttg atagcttaaa atagcttctt cataagctat atccaaataa aaatttctat
     4261 atgcttcgtt ttttagaagg aaacaaaggt cagcattcat agcaagaaag aacatcatca
     4321 tagtaccttc ttcatggtag ttatagatta aagactgcat ttcatttctt atatcttcat
     4381 atattatagc tcctatttcg cttttacttg agaagtaata gcttattaga cctaaattta
     4441 tattagctct ttctgcaatt tgcctagttg ttgtcttatg ataaccttgc tcaaaaaata
     4501 attctctagc agcacttata atactagctt ttgtttcttt gcccttttct gattttgaca
     4561 tatttactgt gtatacctct cttttcaatc tttttttgaa aatttgctaa ctataaatat
     4621 tgtaatagaa ccacctatta taggaaccaa aattctgtca aatacagtaa gaataccaat
     4681 catatttaat aatgccaata taacacttac tataacagca tagatagctc ctttttttgt
     4741 agcacctttc cagcaaagac caattacaaa tggtgcaaat attacacctg tacataatgg
     4801 agctggccaa aacaataatt ctaataaact atcaaattta agtgctatga ttgcacctat
     4861 tatagctgtc aaagctgatg caatttgaga atactttaga gtatttttta aatcattaaa
     4921 ttctatatta ggatgtaata atttgttata aatatctctt gatataatag cactcattga
     4981 ggtcattaat gaatcggcag tagacattat ggcagccaat atagcagcta agaataatac
     5041 agatattaaa ggtggcaaag tagtcgatat catttgaggt aaaacctgaa cagatgatat
     5101 atttggaaat aaactaccac caatagctcc aattattcct atagggaaag caagaaataa
     5161 tagtataaat cctgcaatta cactactttt aactgcattt ttagcattat cagcacattg
     5221 atatcttaaa aaactttcat aagaaaatat tgagcctaaa actaatggta aaactgtata
     5281 cataaaatca ctaatcgatt tatttcctgg aaagaatggt tcttttacca ttgattgtaa
     5341 cataagtaaa gggttgtcgc caactaattt taagcttata taagctacta ttggcaaaaa
     5401 cactatacaa atagcaagtt gaataaatgc tgatactgca actccccaca ttcctgatgt
     5461 ataagtatat ataatgataa ctataactcc tataattaca gctatatcat aaggaatacc
     5521 aaatgcagta gatacagaac caaaagaaat caattgacta cccataatag ctagtataga
     5581 tattggtgtt acaagacttt gataagctct taaaaataca ctatgaccat atctagtttc
     5641 aatataatca gcaggtgtta tatcacaaga taagcttctt aattttcctg cgaaaaatgc
     5701 atttataaag caagctaaag ctgcacctat agtagaatac attccaaccc aaagtcctct
     5761 ttcagctccc cattgaactc caccaactaa gcaccctcct ccaaaagatg tagcagcaat
     5821 ggttgcagct agtataggca aaggcagatt tctacctgcg attaggaaat cggatgctgt
     5881 tttaactttt tttgaaaaat aaaaaccaat ccatatgtat attgcacacg taacaattaa
     5941 aagtaacata gtcttttctc ctttataaaa tattttaata aaatatacaa aatatattta
     6001 aaatatattt tatataaaag tagtacgata tttgttttat tggttttaga ttaatatagc
     6061 tataataatt tatgatatta gatggaaatt acgtaaaaat agaaaattga aaattctgaa
     6121 atttttgcat attgatattt atctcataga ttcttttttg taattgtaac acgcttttat
     6181 ttttaaatca atagtacatg tataaaaata aaaggaattt aaataagaaa aagatatttt
     6241 taatatctgg atgtagta
//
LOCUS       DAMJVK010000159         6249 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.164, whole genome shotgun sequence.
ACCESSION   DAMJVK010000159 DAMJVK010000000
VERSION     DAMJVK010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6249
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.164"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            210..920
                     /locus_tag="PC414_003591"
     CDS             210..920
                     /locus_tag="PC414_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2465942.1"
                     /translation="MTSICICDDEKQQRNMLNKIITREMDLWGYPYQIKEYSNGESLV
                     YNLEHENYYIDIIFLDIELGKSNGVDIAKTIRKHSKDTIIIFVTGFSDYVFHGYDVGA
                     LNYILKPYKTEKICKVLKEALKKLEQWKENFITVQIGTNLCKINTKDILYFKSELRKL
                     TVVTFDKTLEYYGKLNDMETVLPSNFIRTHQRYIVNLNHVDSITQTYAKVQNEKIPIS
                     RKKYQEVVGKFTRYMLES"
     gene            1443..2780
                     /locus_tag="PC414_003592"
     CDS             1443..2780
                     /locus_tag="PC414_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF2465943.1"
                     /translation="MGTYSLISTSINFILILINIFIAFFLYKLTSYFIDFRNHWIFKI
                     ILILGYSIISQMIIWLSDPINILFTLFGYFILLIICSKSKLIPIISVVMILYPIIIGL
                     NFLFINNPIYEELIHSKEYILILSIVQIISALIKLLFWFLIHIFLKNRLVNIKQYLSN
                     KIWIFIDMVCISSFLSILIAIILPPTNLIPMGSNKNIILGNLPYGTYLIILSAILANI
                     GVILLLQPLIENVKMKIEKQANHLKEKYYNLLENQQIQIRKMRHDMNNHFQMIESYLE
                     IEDIAGAKKYFNQLKIGIGTLSGKQFCKNQALNSILNLRHTSLEENGVDVHFNIDIDN
                     HLGIEPIDLCTIFSNSLDNAIEASLKIPDLSNRKVVLKARCEKEYFSYLLTNNKVNPI
                     NRKQELFISDKKDSNSHGYGIENIREIVNKYNGKLDISYTESEFSLFLYIRLF"
     gene            complement(3116..3685)
                     /locus_tag="PC414_003593"
     CDS             complement(3116..3685)
                     /locus_tag="PC414_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HDF2465944.1"
                     /translation="MKKKLEDMTLEELWNLFPIFLTTHKECWKNWFKEEQYRLTGILP
                     MQKIVRISHIGSTSVSTIYAKPIVDILIEIIPDYNMKDIKDLIESNGYICMSESSSRL
                     SLNKGYTVEGFEERVFHLHIRYVGDNDELFFRDYLIDHPDVAKEYEKLKLDLWKKYEH
                     NRDAYTDSKLDFVREFTEKAKVLYSNRYN"
     gene            4055..4660
                     /locus_tag="PC414_003594"
     CDS             4055..4660
                     /locus_tag="PC414_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea carboxylase-associated family protein"
                     /protein_id="HDF2465945.1"
                     /translation="MTNWNLWRTLNMEYIINPCSGIKIKITRGQKIEIVDIEGGQVVD
                     FFAEMKDDSTEFISPGVTIDCNESLRLQVGNLIYSNKYRPMFKILYDDVREHDLLHPC
                     CRPEMYDFFYHNGENHPNCFDNINRTLGTSHQIIHPINLFMYTKVNTDGSIEVCEPLS
                     KANDKIILEALENMTLGIAACSVSESSCNSRKCTSIKILVD"
     gene            complement(5339..6241)
                     /locus_tag="PC414_003595"
     CDS             complement(5339..6241)
                     /locus_tag="PC414_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HDF2465946.1"
                     /translation="MLPQDYYGLIVILGVILGVMGAFYNWFTLYVQSLYKKIPKIGTF
                     GKLLIPFIMAGILGLVMPDVLGSGHNLVEELTSHKYIFGMVVLIFVIRFIFSAVSFGS
                     GAPGGIFFPLLVLGAFIGGIFGMVGVKVFGMNPDYVNNFVLLAMAGYFTAIVRAPLTG
                     IILIFEMTGSVSQMLSLSVISIVAYIVATLMKSKPIYESLLERLLEKNGEPVHKERGE
                     KILDQFSVMFGSPVANKAIQDVQWPENCLLVAIQRGGEEIIPRGKTILRPSDVIVTMT
                     DERDSGAVYDKMENLCKEHTAHIF"
BASE COUNT         2294 a          954 c          741 g         2260 t
ORIGIN      
        1 taataagaaa gattgttaga ttttttaata agaaaatcat tagcacttca gaataagttt
       61 aatagaatag ttttaagtgt aagtatacta atataaaaaa ttttattttg ctatctggat
      121 ataattgtta tttcatttta tatatctagg tagcaaaata tattatatac ttaactatat
      181 aaatataaaa cttgaaagga gataaaaaat tgacttcgat ttgtatttgt gatgatgaaa
      241 aacaacaaag aaacatgctg aataaaatca ttacaagaga aatggatttg tggggctacc
      301 cctaccaaat aaaagaatat tcaaatggag aaagtctagt atacaatcta gaacacgaaa
      361 attattacat agatattatt tttttagata ttgaacttgg aaaaagtaat ggcgttgaca
      421 tagcaaaaac tatacgaaaa cattcaaaag atactataat catctttgtc acaggttttt
      481 ctgactatgt atttcatgga tatgatgtag gagctttgaa ctacatctta aaaccttata
      541 aaactgaaaa aatatgtaaa gtattaaaag aagcactaaa aaaattagaa caatggaaag
      601 aaaactttat tacagttcag attggaacta atctttgtaa aattaataca aaagatatcc
      661 tctactttaa aagtgagcta agaaaattaa ctgtagttac ctttgataaa acattagaat
      721 actacggaaa attaaatgat atggaaactg ttcttccctc taattttata cgtacgcatc
      781 aaaggtatat tgtcaatcta aaccatgtag atagtataac tcaaacttat gccaaagttc
      841 agaatgaaaa aattcctata agccgtaaaa aatatcaaga ggttgtagga aaatttacaa
      901 ggtatatgtt agaatcttaa tttttgaagt tcaatctcta gaattttatt tttgaaactt
      961 aactttttaa agcttagttt ttagagtttt gttttttcaa atttaatttt ttgaagctta
     1021 atttctagaa tttcaatctt tcaaaattta attttttgaa gcttagtttt tagagttttt
     1081 ttcaaactta attttttaaa gctcagtttc tagaatttca atctttcaaa atttaatttt
     1141 ttgaagctta gtttttagag ttttttcaaa cttaattttt taaagctcag tttctagaat
     1201 ttcaatcttt caaaattcaa ttttttgaag cttagttttt agagtttttt tcaaatttaa
     1261 ttttttgaaa cttaattttt aaaatttcaa tcttttaaaa ctcaattttt tgaagcttaa
     1321 tttctagaat tttatttttc aaaatcaatt ctttgaaatc taatttccag aattttaatt
     1381 tttaagattt aattttttaa aacttagttt ccagaatctt aattaataga aaggtatatt
     1441 aaatgggcac ttattcttta atctctacaa gcataaattt tattctaata ttaatcaata
     1501 ttttcatagc cttttttctt tataagttga cttcctattt tatagacttt aggaatcatt
     1561 ggattttcaa aatcatattg attttgggct actctattat tagtcaaatg ataatatggc
     1621 tttccgaccc cataaatatt ttgtttacac tattcggata ttttatacta cttataatat
     1681 gtagcaaaag caaattaatt cctataatat cagttgtcat gatactttat cctattataa
     1741 ttgggcttaa ttttcttttt ataaacaacc ctatatatga ggaacttata cactcaaaag
     1801 agtatatttt aatactttca atagtccaga taatttctgc tctaattaaa ttattatttt
     1861 ggtttttaat acatattttc ctaaagaata gattggtcaa tataaaacaa tacttatcaa
     1921 ataaaatatg gatttttatt gatatggtat gtatatcttc atttttatca atattaattg
     1981 ctattattct gccacctact aatttaatcc ctatgggtag taataagaat attatcttag
     2041 gcaacttacc ttatggcact tatctaatca tcttgtcagc aattctggca aatatcggtg
     2101 tgatacttct tttacagcca ttgattgaaa atgtaaagat gaagatagaa aaacaagcaa
     2161 atcacctaaa agaaaaatac tataacttac tagaaaatca acaaattcaa atacgcaaaa
     2221 tgcgacatga tatgaataat cactttcaaa tgattgaatc gtatttagaa attgaagata
     2281 ttgctggagc taaaaagtat tttaatcagt taaaaatagg tataggtact cttagtggaa
     2341 aacaattttg caaaaaccaa gctcttaact caattttaaa tcttcgtcat acaagtttag
     2401 aagaaaatgg tgttgatgtg cactttaata tagatataga caatcacttg ggtatagaac
     2461 ctatagattt atgtactatt ttttcaaatt ctttagataa cgctatagaa gcatctttga
     2521 aaattcctga tttgtcaaat agaaaagtgg tattaaaggc acgttgtgag aaggagtatt
     2581 ttagctattt attgacaaac aacaaggtaa atcctatcaa tagaaaacaa gaactattta
     2641 tttcagataa aaaagatagt aattctcacg gatatggtat tgagaatatt agagaaattg
     2701 ttaataagta caatggaaaa ttggatattt cttatactga gagtgaattt tctttatttc
     2761 tttatattag attattttag tctaaaatta gattatctta tataatacaa attagattgt
     2821 ttgatactat gtaaaattat aataacttat aattaaatat ttacacatat attcattaac
     2881 ttaaaattta atcaaatgtg atattttatt taccttagta aaaatgtgtg tgtctcaaaa
     2941 taaacttttt agttgatgag gcactctttt ttacatgaaa tcaaatatat ttttatcctt
     3001 tcaacaatga aaaatggatt tatctctcca ttttgaaaca agcccttttt ctattaatta
     3061 ctttcttaaa aaatataata cgatatataa gataaatatc taattgtacc tatttctaat
     3121 tatatctatt agaatacaac acctttgcct tttcagtaaa ctccctaaca aaatctagtt
     3181 tactatctgt ataagcatca cgattatgct catacttctt ccaaaggtcc aacttaagtt
     3241 tttcatactc ctttgcaaca tctgggtggt caattaagta atctctaaaa aacaactcat
     3301 cattatcacc tacatacctt atatgcaaat gaaaaactcg ttcttcaaac ccctctactg
     3361 tatacccttt attaagtgaa agcctactac tgctctcaga catacaaata tatccattcg
     3421 actcaatcaa atctttaata tctttcatat tataatcagg aattatctca atcagtatgt
     3481 caacaatggg cttggcataa atcgtactca cagaagtact tccaatatga cttattctaa
     3541 caattttttg cattggcaat attccagtta gtctatattg ttcttcttta aaccaatttt
     3601 tccaacattc tttgtgagta gtcaaaaata taggaaacaa gttccataac tcctctaaag
     3661 tcatatcttc caacttcttt ttcattagtt acctcctttt aaatttgtac tttatatttt
     3721 ttctttttca aatttactta gttaatattt aactttattt tttatcaaat catttttcta
     3781 attatgtaaa tttttctaaa tttagttttt aatgtattat aacagatttt tacccttctt
     3841 acacttttgt atctatttaa atattagaac attttttctt acttctaaat acagtaagat
     3901 tgcttaaata ccaaatagga gcaaaaaaac tctgtaatct aaaaaaaact acaacatctt
     3961 tatgagaatt aaaaaatgat agactatgtt atcaacatta agaaacagat agaaaagaaa
     4021 cagagtatgt gctaaaatgg gaacatagaa cagaatgaca aattggaatt tatggaggac
     4081 attaaatatg gagtatataa tcaatccttg tagtggtata aaaattaaaa taactagagg
     4141 gcagaaaatt gaaattgtgg atattgaagg aggacaggtc gttgattttt ttgcagaaat
     4201 gaaagatgat tcaactgaat ttatatctcc aggtgttaca attgattgta atgagtcatt
     4261 aagattacaa gttgggaatt taatatattc taataagtat agacctatgt ttaaaatttt
     4321 atatgatgat gtaagggaac atgacttgct acatccatgt tgtagacctg agatgtacga
     4381 tttcttttat cacaatggag aaaatcatcc aaactgtttt gataatatta atcgcacact
     4441 tggaacttcc catcaaatta ttcaccccat taatttgttt atgtacacaa aagtcaatac
     4501 agatgggagt attgaagtat gtgaaccttt atcaaaagcc aatgacaaaa ttattcttga
     4561 agcattggaa aacatgactt tagggattgc tgcatgcagt gtttcagaaa gttcttgtaa
     4621 tagcagaaaa tgtacttcta ttaagatttt ggttgattga caaatttcat ttatcgaaaa
     4681 aatatcaaaa ttaaaaaatg acgtcaaagg cgcaaaaggg taaccacttg cgctctcaat
     4741 tcattatggt ttgttacgta tttatgggta aaaatccctt gattctatga tgtttttcag
     4801 acacaattac aaatgagtaa ggattttata ctcatactct aaaaaatagc attttattgc
     4861 gtaacagaac gaaaaaacag tgtgtacctt tttcttgcaa tacacactgt attcatagcc
     4921 actctagctg ttaactgtta attatctcat aataccctta ctggtattca tctaatctta
     4981 acccctttta attattttca tttataaata aattctattc ttcccaaaat atcgtaatta
     5041 aaatatatta attaaaatat cccaataaga atatctgaaa aatcttctac ctattttttc
     5101 aaacctttta ttcccattcc tcatacaata gtgcaacttt tagcacagta taaatatacc
     5161 attagtccat ataatctcat taacaaatat atacaattat tttcttcaaa ctcttttata
     5221 tctaagatat cacattttca ttttcttata ttaattcctt aactctcttt caaattacca
     5281 attttttaat caaatcttca ttaaatattc tatcattatt aaacattcat cattatccct
     5341 aaaaaatatg tgccgtatgt tctttacaca aattttccat cttatcatac acagcaccac
     5401 tatctctctc atctgtcata gtcacaatta catcacttgg tctaagaatt gtcttacctc
     5461 taggaataat ttcttctcca cctctttgaa ttgctaccaa aagacaattt tcaggccact
     5521 gtacatcctg aattgccttg ttcgcaacag gagagccaaa cataacagag aactggtcta
     5581 gtatcttttc gcccctctct ttatgtacag gttctccatt cttttcaagt aacctctcaa
     5641 gaagactttc ataaataggt ttagacttca taagtgtagc cacaatatat gccacaattg
     5701 aaattactga tagtgaaagc atttgactaa cagaacctgt catttcaaaa attaatatga
     5761 taccagtcaa aggggcacgc acaatagctg taaaatatcc tgccatagcc aaaagcacaa
     5821 agttatttac ataatcggga ttcattccaa aaactttaac acctaccata ccaaatattc
     5881 cacctataaa tgctcctaaa acaagcaatg gaaaaaatat tccccctggt gcacctgacc
     5941 caaaactaac agccgaaaat ataaaacgta taacaaaaat caaaacaacc attccaaaaa
     6001 tatatttatg acttgtaagt tcttcaacta aattgtgtcc actccccaga acatcaggca
     6061 taacaagccc aagaatacca gccattataa atggaatcaa cagttttcca aacgtaccaa
     6121 ttttaggaat cttcttgtat aaagactgca catatagagt aaaccagttg taaaatgccc
     6181 ccataactcc aagaataaca cctaaaatca ctattaatcc ataataatct tgtggcaaca
     6241 catttccaa
//
LOCUS       DAMJVK010000160         6225 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.165, whole genome shotgun sequence.
ACCESSION   DAMJVK010000160 DAMJVK010000000
VERSION     DAMJVK010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6225
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.165"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            174..1196
                     /locus_tag="PC414_003596"
     CDS             174..1196
                     /locus_tag="PC414_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF2465947.1"
                     /translation="MKNLLEIQKKLIPQAIELMERRYSILRQISLSEPIGRRTLSNVL
                     EISERIIRSETEFLKEQGLIDVAVSGMTITKEGSDLLDKLKDIMSDIMGLSKLQDRVR
                     EKLGIKRVILVPGSCDENGSLLKDVTRYGCEYFLGILKDGDIVSITGGSTMLEFSNVI
                     KTDKRYPNSTIVPARGSMGTDVEMQSNNIVASISKKLHSNCKLLHIPDELDESAMKTL
                     SQVPEIKTTLEYIKRTDILVFSIGRADVMAKRRKLPEDKVDEILSKNAVGEAFGYYFN
                     KDGEIVYKLNTVGIDLETVKQVKETIAIFAGTEKVEALLAISNMHKNMVLVTDEESAY
                     KILSLL"
     gene            1256..2263
                     /gene="gap"
                     /locus_tag="PC414_003597"
     CDS             1256..2263
                     /gene="gap"
                     /locus_tag="PC414_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDF2465948.1"
                     /translation="MVKVAINGFGRIGRLALRKMMEQQDKFEVVAINDLTDAKMLAHL
                     FKYDTAQGRFNGEIEVKEGAFVVNGKEIKVTAERNPADLPWAELGVDIVLECTGFFTS
                     KDKAEAHIQAGAKKVVISAPATGDLKTIVFNTNSDILDGSETVISGASCTTNCLAPMA
                     KVLNDKYGIEKGLMTTIHAYTNDQNTLDGPHPKGDLRRARAAAGNIVPNTTGAAKAIG
                     LVIPSLKGKLDGAAQRVPVVTGSITELVCTLGKNVTVEEINAAMKEASNESFGYTEEM
                     LVSSDIIGISYGSLFDATQTKVMEVDGKQLVKVVSWYDNEMSYTSQLIRTLGYFAQLA
                     K"
     gene            2485..3687
                     /locus_tag="PC414_003598"
     CDS             2485..3687
                     /locus_tag="PC414_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HDF2465949.1"
                     /translation="MSMLNKKTIEDIDVCGKKVLVRCDFNVPLQDGVITDENRLNGAL
                     PTIQYLISKGAKVILCSHLGKPKGEAKPELSLAPVAKRLSEMLGKEVVFAADDNVVGE
                     NAKKATEKMENGDVVLLENTRYRKEETKNEENFSKELASLAEIFVNDAFGTAHRAHCS
                     TVGAGEFLQERVCGYLIQKELKFLGEAVANPVRPFTAILGGAKVSDKLAVINELLEKV
                     DNLIIGGGMAYTFLKAQGYEVGTSLLEIDKVEYAKEMMEKAKNKGVNLLLPVDVVMAD
                     HFAPDATPIVTEDANVKEDYMGLDMGPKTIANFVKTIKESKTVVWNGPMGVFEFENFA
                     NGTLSVARAMAELTDATTVIGGGDSAAAVNQLGFGDKMTHVSTGGGASLEFLEGKELP
                     GIAALDNK"
     gene            3713..4456
                     /locus_tag="PC414_003599"
     CDS             3713..4456
                     /locus_tag="PC414_003599"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HDF2465950.1"
                     /translation="MRKPIIAGNWKMHKTIKEALEFVNEVKDKVNSDKVEAVICAPFT
                     LLKDLKEATKGTNIKIGAQNMHFEEKGAFTGEVSPLMLKEIDMDYVVIGHSERRQYFN
                     ETDETVNKKVLKALEVGIDPILCVGETLEQREAGKTKDVCKVQVEKALENVLKDDLAK
                     VVVAYEPIWAIGTGKTATAEDANDVISYIREVIKGLYGELANEVRIQYGGSVKPSNVA
                     EIMGQSDIDGALVGGASLASNDYLDLVNF"
     gene            4465..5997
                     /locus_tag="PC414_003600"
     CDS             4465..5997
                     /locus_tag="PC414_003600"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003228330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HDF2465951.1"
                     /translation="MMKKPVALIIMDGFGYNKDVKGNAIAESKTPNLDRIKKEYPNTL
                     INASGLDVGLPDGQMGNSEVGHTNIGAGRIVYQDLTRITKSIKDGDFFTNKVLCEAMD
                     NAKENSLHVMGLLSDGGVHSHIDHLKAIIKMAKDKGVQKVYVHAFTDGRDTDPQSALE
                     YAKEVQASMDEIGVGEFATVSGRYYAMDRDKRWERVELAYNAMVRGIGEKANSIEEAI
                     QNSYDDGKNDEFIMPTVIMKDDKPVGSIKENDSIIFFNFRPDRARQITRALVCEEFDG
                     FKREDIKNFFVCLTEYDITIENVHIAFGPQSLANTLGEYLAKNGKTQLRAAETEKYAH
                     VTFFFNGGVEEPNKGEERLLIPSPKVATYDLKPEMSAYELTDKALDKLGEDKFDFIVL
                     NFANPDMVGHTGSIEAAIKAVETVDTCVGRLIDKIVELGGSAIITADHGNAEYMLDPE
                     TGKTVTAHSINPVPFIVVGQEYESAKLLDGGRLSDIAPTILDMMKLEKPEEMTGHSLI
                     SK"
BASE COUNT         2482 a          729 c         1250 g         1764 t
ORIGIN      
        1 tactgtgatt tgaaaatggt ggagaaatat tttataaaat aaaaaaataa aaaagttttt
       61 gaaaaactat tgaaatctat acgcaaatcc tataaaataa aaatatcttg tgggacttaa
      121 ataaacacta gggacaaaaa aagtcccgaa aggaaagcaa taaggagtat gttatgaaga
      181 acttattaga aattcaaaag aaattgattc cacaggcaat tgaacttatg gaaagaagat
      241 actctatttt aagacaaata tctttaagtg aaccaatagg taggagaaca ttatcaaatg
      301 tattagagat tagtgaaaga ataatcaggt cagaaactga gtttttaaaa gaacaaggac
      361 ttatagacgt agctgtatct gggatgacta tcactaaaga aggttctgat ttgttagata
      421 aacttaagga catcatgagt gacatcatgg gattgtctaa gttacaagat agagtcagag
      481 aaaagcttgg aataaaaagg gtaatattag ttcctggaag ctgtgatgaa aatggaagtt
      541 tactaaaaga tgtaactaga tatggatgcg aatatttctt aggcatttta aaagatggag
      601 atatcgtttc aataacagga ggaagcacta tgttggagtt ttcaaacgtt attaaaactg
      661 ataagagata tccaaactct acaatagttc cagctagagg aagtatggga actgatgtag
      721 agatgcaatc taacaatata gttgcttcaa taagtaagaa attacattct aattgcaaac
      781 ttttacatat accagatgag ttggatgaaa gtgcaatgaa aactcttagc caagtgccag
      841 agattaagac aactttggag tatattaaaa gaacagacat tttagttttt agtataggta
      901 gagcagatgt catggcaaag agaagaaaac ttccagaaga caaggttgat gagatattat
      961 ctaaaaatgc agttggagaa gcatttggat attatttcaa caaagatgga gaaatagttt
     1021 acaaactgaa cactgttgga attgatttag aaacagttaa acaagtaaaa gaaactattg
     1081 caatctttgc agggactgaa aaagtagaag ccttattagc tatatcaaat atgcataaaa
     1141 acatggttct tgtaacagat gaagaaagtg cttataagat attgtcatta ttataaaagt
     1201 caagacctag cacagagcta gaaaacatac atttacttta taggaggtag cgaaaatggt
     1261 taaagtagct ataaatggat ttggaagaat aggaagatta gcattaagaa aaatgatgga
     1321 gcaacaagat aagttcgaag ttgttgcaat aaacgactta acagacgcta aaatgttagc
     1381 tcatttattt aaatatgata cagctcaagg aagatttaac ggagaaatag aagttaaaga
     1441 aggtgctttt gtagtaaacg gaaaagaaat aaaagtaaca gcagaaagaa atcctgctga
     1501 cctaccatgg gctgaattag gagtagatat agtattagaa tgtacaggat tctttacatc
     1561 taaagataaa gctgaagctc atatacaagc aggggctaag aaagttgtta tatctgcacc
     1621 agctactgga gatttaaaaa caatagtatt caacacaaac agtgacatat tagatggttc
     1681 tgaaacagtt atatcaggag cttcttgtac tactaactgt ttagctccaa tggctaaagt
     1741 attaaatgat aaatacggta tagaaaaagg cttaatgact actatacatg catacactaa
     1801 tgaccaaaat actttagatg gtcctcatcc aaaaggtgat ttaagaagag ctagagctgc
     1861 tgctggaaac atagttccaa acactacagg agctgcaaaa gcaataggct tagtaatacc
     1921 ttcattaaaa ggaaaattag atggggcagc acaaagagta ccagttgtaa ctggttcaat
     1981 aactgaatta gtttgtacat taggaaaaaa tgttacagtt gaagaaataa atgcagctat
     2041 gaaagaagct tcaaacgaat ctttcggata tactgaagaa atgttagtat cttctgacat
     2101 aatcggaata agctacggtt cattatttga cgctactcaa acaaaagtta tggaagtaga
     2161 tggaaaacaa ttagttaaag ttgtttcttg gtatgataat gaaatgtctt atacttctca
     2221 attaataaga actttaggat actttgcaca attagcaaaa tagtcagttt tgagaaaata
     2281 gaaaagtaga ttgacaaagt ccgagtttgt agtttaatac tacggactca ggactttttt
     2341 taagaaaaaa tatataaaat acacctaaat aatgttacat actatatata aaaatatgat
     2401 aatatgatat atttaatagg gtaaaagtat gtactatatt atacttggca ataaaaaatt
     2461 actttattaa aaggagagat atttatgtca atgcttaaca aaaaaactat agaagatatt
     2521 gatgtgtgcg gaaaaaaagt attagtaaga tgtgacttta atgtgccatt acaagatggt
     2581 gtaattactg atgaaaatag attgaacgga gctttaccaa ctattcaata tttaatttct
     2641 aaaggggcaa aagttatatt atgctctcac ttaggaaagc caaaaggaga agctaaacca
     2701 gaattatctt tagcaccagt agcaaagaga ttatctgaga tgttaggaaa agaagttgta
     2761 tttgcagcag acgataatgt tgtaggagaa aatgcaaaga aagcaactga aaaaatggaa
     2821 aacggagatg tagtattatt agaaaatact agatatagaa aagaagaaac taaaaacgaa
     2881 gagaatttct ctaaagaact agcatcactt gctgaaatat ttgtaaatga tgcatttgga
     2941 actgctcata gagcacattg ctcaacagtt ggtgcaggcg agtttttaca agagagagtt
     3001 tgtggatact taattcaaaa agaattaaaa ttcttaggag aagctgttgc aaaccctgta
     3061 agaccattta cagctatact tggaggagct aaagtttctg ataaattagc tgttataaac
     3121 gaattattag aaaaagttga taatctaata ataggtggag gaatggctta tacattctta
     3181 aaagctcagg gttatgaagt aggaacttct ttacttgaaa tagataaagt tgaatatgct
     3241 aaagaaatga tggaaaaagc taagaataag ggtgttaact tattattacc agtagacgtt
     3301 gttatggcag accactttgc accagatgct actccaatag taacagaaga tgctaatgta
     3361 aaagaagatt acatgggact agatatggga cctaagacta tagcaaactt tgtaaaaact
     3421 ataaaagaat caaaaactgt agtatggaat gggccaatgg gagtatttga atttgaaaac
     3481 tttgcaaatg gtacattatc agtagctaga gctatggcag agttgactga tgcaactact
     3541 gtaattggcg gtggagatag tgcagcagca gttaatcaat taggatttgg tgacaagatg
     3601 actcacgtat caacaggtgg aggagcatca ctagaatttt tagaaggtaa agaattacca
     3661 ggtattgcag cattagataa taaataaact aatatataga ggagattgta aaatgagaaa
     3721 acctataatt gcaggaaact ggaaaatgca taaaacaata aaagaagctt tagaatttgt
     3781 aaatgaagtt aaagataaag taaattctga taaagtagaa gctgttatat gtgcaccatt
     3841 tactttatta aaggatttaa aagaagctac taagggtaca aatataaaga taggtgctca
     3901 aaatatgcac tttgaagaaa aaggagcatt tacaggagaa gtttcacctc ttatgttaaa
     3961 agaaatagat atggactatg ttgtaatagg acattcagaa agaagacaat acttcaatga
     4021 aactgatgaa actgtaaaca agaaagtttt aaaagcatta gaagtaggaa tagacccaat
     4081 attatgtgtt ggagaaactt tagaacaaag agaagctgga aaaactaaag atgtttgtaa
     4141 agttcaagtt gaaaaagctt tagaaaatgt tcttaaagat gatttagcaa aagtagttgt
     4201 tgcatatgag ccaatctggg ctattggaac tggtaaaact gcaactgctg aagatgctaa
     4261 tgacgttata tcttatataa gagaagttat aaaaggatta tatggagaat tagctaatga
     4321 agttagaata caatacggtg gaagtgttaa accttcaaat gtagccgaaa taatgggtca
     4381 aagtgatata gatggagctt tagtaggtgg agctagttta gctagtaatg attacctaga
     4441 ccttgttaat ttctaaggag tgaaatgatg aaaaaaccag ttgctttaat tattatggac
     4501 gggtttggat acaataagga tgttaaggga aatgcaattg ctgagtccaa aactcctaat
     4561 ttagatagaa taaaaaaaga gtatccaaac acattgataa atgctagtgg tcttgatgtt
     4621 ggtctacctg atggtcaaat gggaaattca gaagttggac acactaacat aggtgcaggt
     4681 agaatagtat atcaagattt aactagaata acaaaatcta taaaagatgg tgactttttt
     4741 actaataaag ttttatgtga agcaatggat aatgcaaaag aaaactctct tcatgtaatg
     4801 ggacttttgt ctgatggagg agtacattca catatagacc atcttaaggc tataatcaaa
     4861 atggcaaaag ataaaggcgt acaaaaggtg tatgttcacg catttactga tggtagagat
     4921 actgaccctc aaagtgcatt agaatatgcc aaagaagtac aagctagtat ggatgaaatt
     4981 ggagtaggtg aatttgctac agtttctggt agatactacg ctatggatag agataagaga
     5041 tgggaaagag tagaacttgc atacaatgcc atggttagag gtattggtga aaaagcaaat
     5101 tctattgagg aagctattca aaactcttat gatgatggaa aaaatgatga gtttataatg
     5161 ccaacagtta ttatgaaaga tgataaacca gttggaagta taaaggaaaa tgattcaatt
     5221 atattcttca actttagacc agatagagct agacaaataa ctagagcttt agtttgtgaa
     5281 gagtttgatg gatttaaaag agaagatatt aaaaacttct ttgtatgctt aacagagtac
     5341 gatataacta tagagaatgt gcatatagca tttggacctc aatctttagc caacactcta
     5401 ggtgagtatc tagctaaaaa tggaaaaact caacttagag ctgctgaaac tgaaaagtat
     5461 gcacatgtaa ctttcttctt caatggtgga gtagaagagc caaataaagg tgaagaaaga
     5521 ctattgatac cttctccaaa ggttgcaact tatgatttaa aaccagaaat gtcagcatat
     5581 gaattgacag ataaagcttt agataaatta ggagaagata agtttgactt tatagtatta
     5641 aactttgcaa atcctgatat ggtaggtcac acaggaagta tagaagcggc tataaaggct
     5701 gttgaaactg tagatacttg tgtaggaaga ttgatagata aaatagtaga gttaggtgga
     5761 agtgcaataa taactgctga ccacggaaat gctgaatata tgttagaccc agaaacagga
     5821 aaaactgtaa cggctcactc tattaatcca gtaccattta ttgtagttgg tcaagaatat
     5881 gaaagtgcaa aattattgga tggaggaaga ttatctgata tagcacctac tattttagat
     5941 atgatgaagt tagaaaagcc agaagaaatg actggtcatt cacttatttc aaaatagtcg
     6001 attctgataa gtagataata atatataaac tacaatataa atttaaaaat aatgcagttt
     6061 aggtttattt tgtaaagttt agactttatg acataaggtt taaattttgt aagttctgga
     6121 tattgtaagg tgtagatgaa gatatatttt ataaaattta caagatagac aatttataaa
     6181 ataaaaaatt acaatataga taataatgta aataatgata taaat
//
LOCUS       DAMJVK010000161         6175 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.166, whole genome shotgun sequence.
ACCESSION   DAMJVK010000161 DAMJVK010000000
VERSION     DAMJVK010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6175
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.166"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            209..1534
                     /gene="cdeA"
                     /locus_tag="PC414_003601"
     CDS             209..1534
                     /gene="cdeA"
                     /locus_tag="PC414_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter CdeA"
                     /protein_id="HDF2465952.1"
                     /translation="MENLFTRKFTTFEFLKFVSPAIISMIFISLYTIIDGIFVSTLVG
                     SDALASINIVLPIINLVCGFGIMMATGGGAIVSIRMGENRQDEANSTFSFIVLFSLIV
                     GILFTVISYFFIKEISILLGATDKLLPYCITYGKVMILCTPFYILKFIFEYFARTDGN
                     SKFSLFLSVIGGVTNIILDYVFIKYFGMGLLGAAVATAIGIILTCVLGIIYFLSNKST
                     LKLRKPKTDFRLIRDTMINGSSEMVTELSTGITTFLFNVVALKLAGENGLAALTIVLY
                     AHFLMTSVYLGFAAGVSPLISYNFGAENSDKLKETFKHSLKFIFVSSLLVFIFALVFA
                     PFIVRVFVSPDNTVFKLALQGLKIFAFAFLFVGINIFASGFFTAFHNGKISAIISFSR
                     AFVFIIIGIIILPPMLNMTGLWLTVPFAEVITIFISILFIKKYKCRYKY"
     gene            complement(1629..2423)
                     /locus_tag="PC414_003602"
     CDS             complement(1629..2423)
                     /locus_tag="PC414_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2465953.1"
                     /translation="MNELKPYIKFFNKNTKLYIIIFMIVGIVMNILPVFILKIFGEAS
                     KTNLSTIVNPILTYITILLFVYGVNIANKDFSGALSIRADRKSCIKAIVIDLIILSFV
                     ISILGIVLIFLSKLFIEIITGYSIELSVLLKRDMIWTSISALIDPSTSSMPLTYLNYF
                     VQICINEICIGFIGVLLGAFTYRVRKVTSITILIGLPILMIIYIVNFATKNMHKMLLY
                     LEKIIYSFQNPFILFGTKVGIIVICIIFIILLLRKAPIKEYANDLL"
     gene            complement(2424..3299)
                     /locus_tag="PC414_003603"
     CDS             complement(2424..3299)
                     /locus_tag="PC414_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2465954.1"
                     /translation="MSSSIKIENLSHSFKKQNIFNNMSLSFEKNKIYGLLGKNGAGKT
                     TLLNIMVNQLIQNEGEIEILGKNPKEDIKVLEEVCIVREKEFYSPDFKVGQIFEFSSS
                     FYKSYDKGLEEELCKYFDLNTKKKYKQLSRGMKTILSNIIGICSNSAITIFDEPTIGL
                     DAVNRQEFYNIILDSYIKNPRTIIISTHLIDEIDDLLEHVIILNEGKIIIDEEIDVIK
                     QKAHYITGNKEELEKLECIKNIKPKKAFGNAVAYFYYGDFSESDEKILKNSNIDVGYI
                     GLQDMFVNMTKKEEL"
     gene            complement(3303..3671)
                     /locus_tag="PC414_003604"
     CDS             complement(3303..3671)
                     /locus_tag="PC414_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2465955.1"
                     /translation="MKLVLNNEEPIFIQIARAIEDEILSNGIKEEEQVPSTTELSKLY
                     KINPATVLKGINILVDKNILYKKRGIGMFVSDGAKTIIKEARKENFKHNFVKNLLQEA
                     NKLEINREELVDIIINFKED"
     gene            complement(3854..4513)
                     /gene="deoC"
                     /locus_tag="PC414_003605"
     CDS             complement(3854..4513)
                     /gene="deoC"
                     /locus_tag="PC414_003605"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDF2465956.1"
                     /translation="MKHILKTVDHTILKATTTWEDIKILCDEAVDMSVASVCIPPSYV
                     KRASEYLKGKIKICTVIGFPLGYQTTATKVFEAKDAIENGADEVDMVVNISDIKNKDY
                     DNIGKEIKEIKKAIGDKVLKVIIETCYLDEDEKIKMCEIVTMSGSDFIKTSTGMGTGG
                     ATLEDIKLMKEHVGKNVKIKAAGGVKSISDAEKFIEAGAERLGTSSICKILKNEDTTD
                     Y"
     gene            complement(4549..5736)
                     /gene="murJ"
                     /locus_tag="PC414_003606"
     CDS             complement(4549..5736)
                     /gene="murJ"
                     /locus_tag="PC414_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HDF2465957.1"
                     /translation="MKQEPCDFSHGRFRTGIIFIGITSVMSAFLQIKENFIVVGFGSI
                     PYNIVIIISIMLSTIFGPYILPIGAVVAMVVQLLFYMFFVKKTNYKYLYYLNFKDDSL
                     IKLLALLSPVFIGVAVNQVNSLVDTTLASTLVKGSIPALTYADRLNGFVTGTFTASIV
                     SVMYPMLSKLSAENNQKKFTSSVKSSINMIIISMIPISVASIFFATPVVRIIFERGAF
                     DARATQMTATALIFYAVGMTAFGLRDILGKVFYSLQDTKTPMVNGIISVGVNIVLDLV
                     LIKPMAHGGLALATSSSSIACILLLFLNLKRKVGYFGQDKIIKATLKSVVASLIMGVL
                     SYFTYKFIFGILGVGTFNEFVSLAISVIVGGGIYTLLMIIFKVEEVDMILNIAKRKLH
                     LKK"
     gene            complement(5813..>6175)
                     /locus_tag="PC414_003607"
     CDS             complement(5813..>6175)
                     /locus_tag="PC414_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2465958.1"
                     /translation="QKLSTKLIKENDIICIEDLQVKNMIRNRKLSRLISDVSWSEFIR
                     QLEYKANWYGRQIVKVGKFFASSQICNKCGYKNEEVKNLNIREWICPSCNETHDRDIN
                     ASINILKEGLRLITIQNK"
BASE COUNT         2047 a          967 c          681 g         2480 t
ORIGIN      
        1 aagattatat acatatattt ctaaagattt tcttaagaaa cagttagatt ttactaatat
       61 aagtttatta attagcatta tactaattta tcttaatatg attttttgtt gattttaagc
      121 tgtttatcgt ataaataaaa tttttcttga ctatatagtt actatatagc ttattatata
      181 tagagagtaa taaaaggagg taaaatttat ggaaaattta tttacaagaa aattcactac
      241 ttttgaattt ctaaaatttg tttctcctgc aattatatcc atgatattta tatctttgta
      301 cacaataata gatggcatct ttgtatcgac attagttggt tctgatgctc ttgctagtat
      361 aaatattgta ctacctataa ttaaccttgt ttgtggattt ggaataatga tggcaactgg
      421 tggaggtgct atcgtttcta tacgtatggg tgaaaataga caggatgaag ccaactctac
      481 attttctttt atagttttgt tttcattgat tgttgggatt ttattcacag taatctcata
      541 tttctttatc aaagaaatat ctattttgct tggtgcaaca gataagttat taccatattg
      601 tataacttat ggtaaagtta tgattttatg tacaccattt tatattttaa aatttatatt
      661 tgagtacttt gcaagaactg atggaaattc taaatttagt ttatttctat cagtcattgg
      721 tggtgtaaca aatataatat tggattatgt atttattaaa tattttggaa tgggtctttt
      781 aggagctgca gttgcaactg ctataggtat tattttaact tgtgttttag gtattattta
      841 cttcttatct aataaatcta cactaaaatt aagaaaacca aaaaccgatt ttagacttat
      901 aagagatact atgataaatg gttcttctga aatggttaca gaattatcta caggaattac
      961 aacattctta tttaatgtag tagctttaaa attagcagga gaaaatggac ttgctgctct
     1021 tactatagta ttgtatgctc attttttaat gacatcagtc tatctaggat tcgctgctgg
     1081 agtgtctcca ttaataagct ataattttgg tgctgaaaac agtgataaat taaaagaaac
     1141 atttaaacat tctctaaaat ttatatttgt ttcttctctt ttagtgttta tttttgcttt
     1201 agtatttgca ccatttattg ttagggtctt tgtaagtcca gataacacag tatttaaact
     1261 agccttacaa ggattaaaaa tatttgcatt tgcttttttg tttgttggta taaatatatt
     1321 tgcatcagga ttttttacag catttcacaa tggaaaaatt tcagctatta tatcttttag
     1381 tcgtgccttt gtttttataa tcataggaat cataattctt cctcctatgt taaacatgac
     1441 tggattatgg cttacagttc catttgctga ggttataacc atatttatat ctattctatt
     1501 tataaaaaaa tataaatgta gatataagta ttaaaaatta atacttaaaa tcaacattta
     1561 ttaaaactaa taaacctgct ttaaaataca aatttacaaa tttgattttt aagcaggttt
     1621 tattttagtt ataataagtc atttgcatac tctttaattg gtgcttttct aagtaataaa
     1681 attataaata ttatgcaaat aactataatt cctacttttg ttccaaataa aataaaagga
     1741 ttctgaaatg aataaataat tttttctaaa tacaatagca ttttatgcat atttttagtt
     1801 gcaaaattaa ctatataaat tatcattaat atcggtaatc ctatcaatat agtaatacta
     1861 gtaacttttc taactctata tgtaaaagct ccaagtaaca ctcctataaa acctatacat
     1921 atttcattta tacatatttg tacaaaataa tttaaataag ttagtggcat acttgatgtt
     1981 gaaggatcta tcagtgctga tatagatgtc caaatcatat ctctctttag taaaactgat
     2041 agttctatac tgtaacctgt tataatttct ataaataact tcgataaaaa aatcaaaact
     2101 atacctaaaa tagaaatcac aaagcttaaa ataattaaat ctatgacaat agcttttata
     2161 caactttttc tatctgctct tatacttaaa gctcctgaaa agtctttatt tgctatattt
     2221 actccataca caaatagcag tatagttata tatgttaaaa taggattcac aatcgtggat
     2281 aaattagttt tagatgcttc tccaaaaatc ttgagaataa acactggcaa aatattcata
     2341 acaattccca caatcataaa tataattata tacaatttag tatttttatt aaaaaatttt
     2401 atatatggct ttaattcatt caattacaat tcctccttct tagtcatgtt aacaaacata
     2461 tcttgtagcc ctatgtatcc tacatcaata tttgaatttt taagtatttt ttcatcactt
     2521 tcactaaaat caccatagta aaaataagca actgcatttc caaatgcctt tttaggtttt
     2581 atatttttta tacattctaa tttttctaac tcttctttat ttccagtaat ataatgagct
     2641 ttttgtttta tgacatcaat ttcttcatct ataataattt ttccctcatt taatattatt
     2701 acatgctcca gtaaatcatc tatttcatca atcaaatgag ttgatattat tattgtcctt
     2761 ggatttttta tataactatc taaaattatg ttataaaact cttgtctatt tactgcatcc
     2821 agaccaattg ttggctcatc aaatatggtg atagctgaat tagaacaaat acctattata
     2881 tttgaaagta tagttttcat acctcttgac agttgtttat actttttttt agtatttaaa
     2941 tcaaaatatt tgcataattc ttcttctaag cctttatcat aacttttata aaaactagaa
     3001 gaaaattcaa atatctgacc tactttaaaa tctggactat aaaattcttt ttccctaacg
     3061 atacaaactt cttccaaaac ttttatatct tcttttgggt tctttcctaa tatttctatc
     3121 tcaccttcat tttgaattaa ctgatttacc attatattta ataatgttgt ttttccagct
     3181 ccattcttac ctaataaacc atatatctta tttttttcaa agcttagaga catattatta
     3241 aaaatatttt gcttcttaaa actgtgagat aaattttcta tcttaataga actactcata
     3301 tattaatcct ccttgaaatt aattataata tcaaccaatt cttctctatt tatttctaat
     3361 ttgttagctt cttgaagtag gttttttaca aaattatgtt taaaattttc ttttctagct
     3421 tcttttataa tagtttttgc accatctgat acaaacatcc ctatccccct cttcttatat
     3481 aaaatatttt tatcaaccaa tatatttata ccttttaata ctgtagctgg attaatttta
     3541 taaagctttg aaagctctgt tgttgatgga acctgttctt cttcttttat tccatttgat
     3601 agtatctcat cttcaattgc tcgtgctatc tgaataaata taggttcttc attatttaat
     3661 actaatttca taggttcctc ctttctccat cagttagtta ctcaactaat taactatata
     3721 acttataata tcttgaaaaa tattttctgt caatactaaa taaaaaacag agggaaaatt
     3781 ccctctgttt tttgttcttt atatttataa ttaaattttg atttttgtaa tcctatccca
     3841 taagttaaaa actttaataa tctgttgtat cttcattttt taatatttta catattgagc
     3901 tagttcctaa cctttctgct cctgcctcta taaatttttc tgcatcagaa attgatttaa
     3961 ctccccctgc agcttttatt tttacatttt ttccaacatg ctctttcata agttttatat
     4021 cttctaaagt agctccacct gttcccattc cagtagatgt ttttataaaa tctgaacctg
     4081 acattgttac tatttcacac atttttattt tttcatcttc atctaaataa catgtctcta
     4141 taatgacttt taatacttta tctcctatag ctttttttat ttcttttatt tcttttccta
     4201 tattatcata atctttattt tttatatctg aaatgtttac aaccatatca acttcatctg
     4261 ctccattttc aatagcatct tttgcttcaa atacttttgt agcagtagtt tgatatccca
     4321 aagggaaccc tatcactgta catatcttta ttttaccttt tagatattct gatgctcttt
     4381 tcacatatga tggtggtatg caaactgatg ctacactcat atctacagct tcatcacata
     4441 aaattttaat atcttcccat gtagttgtag cttttaaaat agtatggtct actgttttta
     4501 atatatgttt cataaaatcc tcctaaattt ataattaata taattagatt acttttttaa
     4561 atgcaatttt ctctttgcaa tgtttagtat catatcaacc tcttcaactt taaaaataat
     4621 cattagtagt gtataaattc cacctccaac tataactgaa atagctagtg atacaaactc
     4681 attaaatgtt cctactccca aaattccaaa tataaattta tacgtaaagt atgataaaac
     4741 acccataatt aaagatgcaa ctaccgattt caaagttgct tttattattt tatcttgtcc
     4801 aaaatatcca acttttctct ttaggttcaa gaagagtaac aatatacatg caattgatga
     4861 tgaactagtt gcaagagcaa gaccaccatg agccattggt tttattaaaa ctaagtccaa
     4921 tactatattt acccctacag atataatccc atttaccata ggtgtttttg tgtcttgtaa
     4981 tgaataaaaa acctttccca aaatatctct taagccaaat gcagtcatac caactgcata
     5041 aaaaattaag gcagttgctg tcatttgggt tgccctcgca tcaaaagcac ctctctcaaa
     5101 aatgattcta acaacaggag ttgcaaaaaa aatagaagca actgaaatag gaatcataga
     5161 aattattatc atatttatac tggatttaac tgaactagta aacttctttt gattattttc
     5221 tgcacttaac ttagataaca taggatacat aacagatact attgaagctg taaatgtccc
     5281 tgtaacaaaa ccatttaatc tatctgcata agtaagtgct ggaatgcttc ctttaactaa
     5341 tgttgaggct aaagttgtat ctaccaatga gttcacttga ttaacagcaa cacctataaa
     5401 tactggtgaa agtaatgcta gtaatttaat aaggctatca tctttaaagt ttaagtagta
     5461 taagtatttg tagttcgttt ttttaacaaa aaacatgtaa aagagcagtt gaactaccat
     5521 agcaaccaca gcaccaattg gaagtatgta tggtccaaat atggtactta acattattga
     5581 aataattatt actatattat agggtatact tccaaatcca actacaataa agttctcttt
     5641 aatttgtaaa aatgctgaca taacactcgt aatacctatg aatattattc ctgttctgaa
     5701 cctcccatga ctaaagtcac agggttcctg cttcatagaa ttttgcatac taaaatatgt
     5761 cttactaatt ctccacaggc tatccccgta gttcctacgg ttcttacata cgttatttat
     5821 tttgaattgt tattagtctt aatccttctt ttaatatatt tatacttgca tttatatctc
     5881 tatcgtgagt ttcattacaa ctaggacaaa tccattctct tatatttaag ttcttaactt
     5941 cctcgttttt atacccacat ttattgcata tttgtgaact tgcaaagaat ttacctacct
     6001 ttacaatttg tctaccatac caattagcct tgtattctaa ttgacgaata aactctgacc
     6061 atgatacatc agaaattaag cgagatagtt tacgatttct aatcatattc tttacttgta
     6121 aatcttctat gcaaataata tcgttctctt ttattaattt agtagatagt ttttg
//
LOCUS       DAMJVK010000162         6162 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.167, whole genome shotgun sequence.
ACCESSION   DAMJVK010000162 DAMJVK010000000
VERSION     DAMJVK010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6162
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.167"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            287..1654
                     /locus_tag="PC414_003608"
     CDS             287..1654
                     /locus_tag="PC414_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HDF2465959.1"
                     /translation="MGLKEFDFIEESIDMLRTMSPTLETISDEIEEYFENILDEKNQE
                     YINVTSRIKSESSLREKIIRNRYLKKYGEASNLIHNVSDLIGLRIECRFIEDENKIYR
                     LLRRYFNKTDDKINYYNKENKNIKLKLSERQPNKQKNGFEIYKIDGVFAYLDREVKFE
                     LQIKSLVNVFWSEIEHKIIYKNNTYLLADKFIKDMMDSIKNNLTMIDNQLLSIYKNFH
                     SGKSFNMKVSKKEIEKLFAKLVYDAFSEKMNKSIGFVVDFKKPCETILNYSFNKQDIS
                     DEVLGNFMLDEFARLNEIVNKDIDFNEQIEFEREPTFDDKFCKDLGNHFRSRLNTEFP
                     WNLFFRILFEIEPYNNTKDFENFVEFIKENVLLEENREQLLCQFEEDSKLIIEDIYDC
                     IFNSISEIDSVEILYNYNLEKINFTSSEIINCICREYECYGEYLEEKEKLMNTFKEKI
                     IQIFE"
     gene            1679..2287
                     /locus_tag="PC414_003609"
     CDS             1679..2287
                     /locus_tag="PC414_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HDF2465960.1"
                     /translation="MRHFSNIYELSKIILEEAICIEKTGVILAGGKNSRMGRDKAFLE
                     LHDKLFIEIAIEAFKNFDELIIISNNEELYSKYDIKVYNDIVKDVGPIGGIYTALEYA
                     KYDIVTIACDMPYLNNQIVERIANKMDDKSVISVTNGKLQPLCSGYKKSIINKVSLCI
                     RENDLKLRSFIDKIDKSYIYFDEEDLFLNVNTVDEYKKLTKV"
     gene            2335..2706
                     /locus_tag="PC414_003610"
     CDS             2335..2706
                     /locus_tag="PC414_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HDF2465961.1"
                     /translation="MNLLKCDSWAVILNNSDKESKAYKILDELKRNMYKVVAIDEEKK
                     PIEGIDVYECLKDVPHNIDVVAIIDKQSKMDVILEEVELLDIQNMWFEKGSFSEMMIK
                     KTKDLKLNIEYNLSLYDELTR"
     gene            2885..3223
                     /locus_tag="PC414_003611"
     CDS             2885..3223
                     /locus_tag="PC414_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HDF2465962.1"
                     /translation="MLIVTTEKVEGKKISKVLGLVRGSTIRAKHVGKDIGASFKNLVG
                     GELTGYNEMLTEARQIAIGRMVEDAEAKGANAVIAFRLSSASVMQGAAEMLAYGTAVV
                     LEDDNSILEK"
     gene            3689..4192
                     /locus_tag="PC414_003612"
     CDS             3689..4192
                     /locus_tag="PC414_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HDF2465963.1"
                     /translation="MFNVAIITLSDKGYEGKREDITGKKLTEFVENTGVYKVTEYVLI
                     KDDKEMLKENIIRLCNSNKIDLILTNGGTGFSKRDITPEATKEVLEKEIPGLSEYMRM
                     KSTEITKKAILSRGVSGIRNNSIIINLPGSPKGAVENLSFIIDVLDHGIEVLRGEATE
                     CATKKSE"
     gene            4239..5198
                     /gene="moaA"
                     /locus_tag="PC414_003613"
     CDS             4239..5198
                     /gene="moaA"
                     /locus_tag="PC414_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HDF2465964.1"
                     /translation="MIDKYGRKIDYLRISLTDKCNLRCVYCMPPDVKFDKNYINENLS
                     FEDYKFIIKAMAEQGITKIRFTGGEPLLYPKLNELIKFTKEECGINNIGMTTNAIGLS
                     DRIFELEKCGLNKVNISLDSLKEYKYKSITRGGNLKDVLKSIESCLNLGIKVKLNCVA
                     ISGFNDDELKDFMFLTVKSPIDVRFIELMPLGEANRVYKKGYFNVKEMIEKIYGLYKV
                     KNDEKSTAEYYMFKGAKGRIGIITPLSCSFCNTCNRIRLTSSGAIKLCLHSKEEIDIK
                     PFLHKPLLFRESMKDIIKQKPEKHHLIENKCSDTNRRMYEIGG"
     gene            5202..5672
                     /gene="moaC"
                     /locus_tag="PC414_003614"
     CDS             5202..5672
                     /gene="moaC"
                     /locus_tag="PC414_003614"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HDF2465965.1"
                     /translation="MLTHINEKGYAKMVDISEKDDTKRTAVATATITLSKEALEKVKN
                     GQIKKGDVLSVAQVAGIMGAKNTSSNIPMCHQIKLVGCDLEFDVDDSMSAIKIYATCK
                     SLGKTGVEMEALNACSLAALTIYDMCKAVDKKMVISNIHLVEKTGGKSGDFKFE"
     gene            5708..6139
                     /locus_tag="PC414_003615"
     CDS             5708..6139
                     /locus_tag="PC414_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HDF2465966.1"
                     /translation="MAKVVSINISEKKGVIKVPVQCAELKINHGIVGDAHAGNWHRQI
                     SLLGKESIDKMKMKGFDQLKYGDFAENVTTEGIEVFSLPVGTKLRIGQCEVEVTQIGK
                     KCHNGCEIKKLTGDCVMPREGIFVKVLKEGIINVNDSIDVI"
BASE COUNT         2603 a          501 c         1118 g         1940 t
ORIGIN      
        1 aatttttata tagtaaatga caaaagttta caataaatgt atattttctt actcattcct
       61 taaaattact catttagttt aataactctc atttcatatg taaagttggt aaaaaaatta
      121 aaattaaaag taaaaaacta aataaattaa tgttaatttg aaatatatat gaaggttttt
      181 ggagaatact tacttatgat aggaaaaaat gttaaaataa tagtgtaatc aaatataaag
      241 gactgaaata attaacagtt attaaatatt aggagtgtaa aaatttatgg gattaaaaga
      301 atttgatttt attgaagagt ctattgatat gttgaggaca atgtcaccaa cattagaaac
      361 tatatcagat gaaatagaag aatattttga aaatatcttg gatgaaaaga atcaagaata
      421 tataaatgta acctctagaa taaaatcaga atcaagttta agagaaaaga taattagaaa
      481 tagatattta aaaaaatatg gagaagcaag taatcttatt cataatgttt ctgacttaat
      541 aggcctaaga attgagtgta gatttattga ggatgaaaac aaaatatata gacttttgag
      601 aagatatttt aataaaacag atgacaaaat aaactattat aataaagaga ataagaatat
      661 taaattaaaa ctatctgaga ggcaacccaa taagcaaaaa aatggatttg aaatatataa
      721 aatagatgga gtttttgcgt atttagatag agaagtaaaa tttgaacttc aaattaaatc
      781 tcttgtaaat gtgttttgga gtgaaataga acataaaata atttataaaa acaatacata
      841 tttacttgct gataaattta taaaggatat gatggactct ataaagaata atcttactat
      901 gatagataat cagcttttaa gtatttataa aaactttcat tcagggaagt cttttaatat
      961 gaaagtaagt aaaaaagaaa ttgaaaaatt atttgcgaaa ctagtctatg acgctttttc
     1021 tgaaaagatg aataaaagta taggttttgt tgtagatttt aaaaaaccat gtgagactat
     1081 attgaactat tcttttaata aacaagatat aagtgatgaa gttttaggta attttatgct
     1141 tgatgagttt gcaagattaa atgaaattgt aaacaaggat atagatttta atgagcaaat
     1201 agaatttgaa agagaaccaa catttgatga taagttttgt aaagacctag gaaatcattt
     1261 tagaagtaga ttaaacacag aatttccatg gaacctattt tttagaatat tatttgaaat
     1321 agagccatat aacaatacca aagactttga aaactttgta gagtttataa aagaaaatgt
     1381 gctactagaa gaaaataggg agcaattatt atgccaattt gaagaggata gtaagcttat
     1441 aattgaagat atatatgatt gtatatttaa tagtataagt gagattgata gtgttgaaat
     1501 tttatataac tataatcttg aaaaaataaa ctttacatca agtgaaatca taaattgtat
     1561 atgtagagaa tatgagtgtt atggtgaata cttagaagaa aaagaaaagc ttatgaatac
     1621 atttaaagaa aagattattc aaatctttga gtaataggtc aatgctgaag tggcgctaat
     1681 gcgccatttt tcaaatatat atgaattaag taaaattatt ttggaggagg cgatttgtat
     1741 agagaaaaca ggtgtaattt tggcaggggg aaaaaattct agaatgggta gagataaagc
     1801 ctttttagag ttacatgata agctttttat tgaaattgca atagaagcat ttaaaaactt
     1861 tgatgagttg ataataatat ctaacaatga agaattatat tctaaatacg atattaaagt
     1921 ttataatgat atagtaaaag atgttggtcc aattggagga atatatactg ctcttgaata
     1981 tgcaaagtat gatatagtta caattgcatg tgatatgcca tatctaaata atcaaatagt
     2041 cgaaagaata gctaataaaa tggatgataa aagtgtgatt tcagtgacaa atggaaagtt
     2101 acaaccattg tgttctggat ataaaaagag tataataaat aaagtatctt tatgtataag
     2161 ggaaaatgat ttaaagctca gaagctttat agataaaata gataaaagtt atatatactt
     2221 tgatgaagaa gatttatttt taaatgtaaa cactgttgat gagtataaaa aattaactaa
     2281 agtttaatta gctatggttg taaaatagag taggatttat atggaggaga gtttatgaat
     2341 ttacttaaat gcgattcttg ggcagtaata ttaaataata gcgataaaga aagtaaagca
     2401 tataaaatat tagatgaact taaaaggaat atgtacaaag ttgttgctat agatgaagag
     2461 aaaaaaccta ttgaaggaat agatgtgtat gaatgtttaa aagatgttcc acacaatatt
     2521 gatgttgtag ctattattga taaacaatct aagatggatg tgattttaga agaagtagag
     2581 cttttggata tacaaaatat gtggtttgaa aaaggaagct ttagtgaaat gatgattaaa
     2641 aagactaaag atttaaaatt aaatatagaa tataatttaa gtttatatga tgagttgact
     2701 agataaaatt atatttctta ataataaaaa tataattagt taataaaaca tattatttta
     2761 tagtatagat tggttaataa aagtacagat tggttaatga aacattaaat tacaatattt
     2821 gttaaaaaaa ttgtgagtta catgatataa tgtattaaat aggatactaa ggagttgata
     2881 tattatgtta atagtaacta cagaaaaagt agaaggtaaa aagatatcaa aggttttagg
     2941 attagtgaga ggaagtacaa taagagcaaa acatgttgga aaagatatag gagcaagttt
     3001 taagaatctt gttggaggag aacttactgg atataatgaa atgctcactg aagcaagaca
     3061 aattgccata ggtagaatgg ttgaagatgc agaagctaaa ggtgcaaacg cagtaatagc
     3121 atttagactg tcttcagctt cagttatgca aggagcagca gaaatgcttg cttatggaac
     3181 agcagttgtt ttagaagatg ataatagtat tcttgaaaaa taaaatataa atagttaaaa
     3241 agaggcatct tttaaaaatt ttatggatgc ctttttttat ttgaaaaaac taatttgaga
     3301 agaaatttta actattatga caataaaatt agggtgatat tatataaata agtaaaatta
     3361 tattatataa tgtatggtat agactttaaa aattttattt gtagagaaaa atatggacaa
     3421 tgttataata attatagaaa ttaaaagtac ttacattcat tatgtatgtg acatgtataa
     3481 ttagttttat atttaataca gttttgaagt aatatcttca ttttgagaac gttggaataa
     3541 taaaaaatgt attattatag gtgatataaa taaaaaataa atataatatg ttatattgct
     3601 gatagaaaag agaataaaag aaaataggtt ttattaaaag agatttttat ttataggact
     3661 taatcaatgt atagaggggt gagaacatat gtttaatgtt gcaattataa cacttagtga
     3721 taaaggatat gaaggaaaaa gagaagatat aacaggaaaa aagttaactg aatttgttga
     3781 aaatacagga gtttataaag ttactgaata tgtacttata aaagatgata aggaaatgct
     3841 aaaggaaaat attataagac tttgtaatag taataaaata gatttaattc ttacaaatgg
     3901 aggtactggt ttttcaaaaa gagatataac cccagaagca actaaggaag tgttggaaaa
     3961 agaaatacca ggtctgagcg aatatatgag aatgaaatcc acagaaatta ctaaaaaggc
     4021 catactaagt aggggtgtga gtggaataag aaataattct ataataataa atttacctgg
     4081 aagtcctaaa ggtgcagttg aaaatctaag ttttataatt gatgttttag accatggcat
     4141 agaagtttta agaggagaag ctacagaatg tgccactaaa aaaagtgaat agtatataat
     4201 ctagatagat aaaaaataga caaagaggta gataaagtat gattgataag tatggaagaa
     4261 aaatagacta ccttcgtatt tcacttacag ataagtgtaa tttaaggtgt gtctattgta
     4321 tgccaccaga tgtaaaattt gataaaaatt atataaatga aaatctaagc tttgaagact
     4381 acaagtttat tataaaggct atggctgaac aaggtataac aaaaattaga tttactggag
     4441 gagaacctct tttatatcct aaattaaatg agcttataaa atttacaaaa gaagaatgtg
     4501 gaataaataa cataggaatg accactaatg ctattggtct ttctgataga atttttgagc
     4561 ttgaaaaatg tggcttaaat aaagttaata taagtttaga ttctttgaag gaatataagt
     4621 ataaatccat aacaagagga ggaaacttaa aagatgtctt aaaatcgatt gaatcttgtt
     4681 taaatttagg tataaaagta aagttaaatt gcgttgctat aagtgggttt aatgatgatg
     4741 agcttaaaga ttttatgttt ttaacagtta aatcaccaat tgatgtaaga tttatagagc
     4801 ttatgccatt aggtgaggct aatagagttt ataagaaggg ttattttaat gtaaaagaaa
     4861 tgattgaaaa aatatatggt ttatataaag tcaaaaatga tgaaaaaagt acagcagaat
     4921 attatatgtt taaaggagct aaaggaagaa taggaattat aactccatta agctgttcat
     4981 tttgtaacac ttgtaatcgt attagactta cttctagtgg tgcaataaaa ttatgtctac
     5041 attcaaaaga agaaatagat ataaaaccat ttttacataa accattgtta tttagagaat
     5101 ctatgaaaga tataataaaa caaaaaccag aaaaacatca tttaatagaa aataaatgta
     5161 gtgatacaaa taggcgtatg tatgaaattg gaggataagt aatgttaaca catataaatg
     5221 aaaaagggta tgccaagatg gtggacataa gtgaaaaaga tgatactaaa agaacggccg
     5281 tagctacagc tacaattact cttagtaagg aagcattaga aaaagtaaaa aatggtcaga
     5341 taaaaaaagg agacgtattg tctgtagctc aagttgcagg tataatggga gctaaaaata
     5401 catcttcaaa cataccaatg tgccatcaga ttaaattagt tggatgtgat ttagaatttg
     5461 atgtagatga ttctatgtct gctattaaga tatacgcaac ctgtaaatct cttgggaaaa
     5521 ctggagtaga aatggaagct ttaaatgcat gttctttagc agcacttaca atttatgata
     5581 tgtgtaaagc agttgataaa aaaatggtta taagtaatat ccatcttgtg gagaagactg
     5641 gtggaaaatc aggagatttt aagttcgagt aaattaataa tatattgggt taaaatttgg
     5701 aggaaatatg gcgaaggtag tgtcaataaa tataagtgag aaaaagggtg taattaaagt
     5761 tcctgtacaa tgtgcagagc taaaaataaa tcatgggata gttggagatg cacatgcagg
     5821 aaactggcat cgtcagataa gcttacttgg aaaagaaagc atagataaaa tgaaaatgaa
     5881 aggctttgac caattaaaat atggagattt tgctgaaaat gtaactacag aagggataga
     5941 agttttttcc ctgcctgttg gtacaaagtt aagaataggg caatgtgaag ttgaagtaac
     6001 tcaaataggg aaaaaatgtc acaatggatg tgaaattaag aaattgacag gtgactgtgt
     6061 gatgccacga gaaggaattt ttgtaaaggt tctaaaagaa ggaattataa atgtgaatga
     6121 ttcaatagat gtaatctaat gaagtcacta ttaaaatata at
//
LOCUS       DAMJVK010000163         5836 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.168, whole genome shotgun sequence.
ACCESSION   DAMJVK010000163 DAMJVK010000000
VERSION     DAMJVK010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5836
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.168"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(322..2154)
                     /locus_tag="PC414_003616"
     CDS             complement(322..2154)
                     /locus_tag="PC414_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2465967.1"
                     /translation="MSRMGRGPMGKSMGAGQKANDFKGTMRKLIAYLSKFKISIILVI
                     VFAIGSASFSIVGPKILGKATTKIFEGLVSKVSGGNVGIDFNAIGKILTFLLFLYLIS
                     ALFSFIQGFIMSGISQKVSYNLRKEISAKLDRLPMKYFDTKTHGEILSRITNDIDTLN
                     QSLNQSMTQLITSVTTMIGVLIMMLSISGIMTLVAVLILPISMFVISRIIKKSQKYFR
                     YQQEYLGNVNGQVEETYSGQTIVKAFNREDEVIEEFDKLNDSLYNSAWKSQFLSGIMQ
                     PLMMFIGNLGYVMVSILGGWLAIKKTIEVGDIQSFIQYVRNFTQPMTQIAQVANLLQS
                     TAAASERVFEFLEEEEEVQIVENAVSIDGLEGKIDFENVNFGYNPNKTIINDFSVNVK
                     PGQKVAIVGPTGAGKTTIVKLLMRFYDVNSGSILIDGHNIKDFNRSELREMFGMVLQD
                     TWLFSGSIMENIRYGKLNATDEEVIEAAKSAHVHRFIKTLPDGYKMKLNEEASNVSQG
                     QKQLLTIARAILADPKILILDEATSSVDTRTEVLIQKAMDNLMEGRTSFVIAHRLSTI
                     RDADMILVMNEGDIVEQGNHEELLKKGGFYANLYNSQFEEDEAM"
     gene            complement(2147..4393)
                     /locus_tag="PC414_003617"
     CDS             complement(2147..4393)
                     /locus_tag="PC414_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2465968.1"
                     /translation="MFKLIKYLKKSALSIVIIVCLLVIQAVCDLSLPEYTSNIVNVGI
                     QQGGVENSVPSVIRESELNKITLFMDKSSKDKVLDNYTLLNKKDYVKYKDKYPGLKDE
                     SLYELNTKDKDTIDDLNVIFGKAILIVSGLEGDSKEVKAMKAQLMSKLPPQATQSGDV
                     DIFKLLSAMPKEQLDTLTKEMSKGFESMPESMITQSSVSYVRSEYEKIGIDTEKTQNN
                     YILFTGAKMLGIAMISMVATITVGFLAARVAASVGRNLRSGVFRKVMSFSNTEMNEFS
                     TASLITRSTNDIQQIQMLMVMLLRIVFYAPIMAIGGVIKVLNTNTSMAWIIAVAVVAI
                     LSLIVVLFSVVMPKFKLVQKLVDKLNLVSREIITGIPVIRAFSTEKHEEERFDKANKN
                     LTKTNLFVNRVMTCMMPAMMLIMNLITVLIVWRGAYSVDSGAMQVGDMMAFIQYTMQI
                     IMSFLMISMVSIMLPRASVSATRIDEVLVSDISIKDPEKSEAQEFKEDKKGLVEFKNV
                     SFMYPDAEEPILTNISFTAKPGETTAFIGSTGSGKSTLINLIPRFFDVTEGEILLGGV
                     NIKNVSQHDLREKIGYVPQKGVLFSGTIESNLKYGRKDATDEEMRTAAEIAQATEFID
                     SKPEAFKTEISQGGTNVSGGQKQRLSIARAIAKNPEVYIFDDSFSALDLKTDAALRKA
                     LKSKTAESTVLIVAQRISTILNAEQIIVLNEGEIVGIGTHKELLKNCEVYEQIALSQL
                     SKEELANE"
     gene            complement(4461..4922)
                     /locus_tag="PC414_003618"
     CDS             complement(4461..4922)
                     /locus_tag="PC414_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2465969.1"
                     /translation="MNSNSEEKLNDVFLQLMRIYFIRTHFYFDKLGLYHGQPQILFSL
                     KMKDGQSQKELADKRKVKASTTTVMIKRMEKAGLVERKQDEKDQRVSRIFLTDKGLEI
                     CNKVEEFNKELEREFFLGFSEEEKIVLRRLLMQVRDNLKAGCHKHEDEKYF"
     gene            complement(5227..5793)
                     /locus_tag="PC414_003619"
     CDS             complement(5227..5793)
                     /locus_tag="PC414_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HDF2465970.1"
                     /translation="MRKLEEILEYNKSFVENKEYEQYVTSKHPNKKIVVLSCMDTRLT
                     ELLPKAMNLKNGDVKLIKNAGATIMHPFGSIMRSVLVAIYEFDVDEVMVVGHHGCGMC
                     NVDTDKLLGKILDRGISNDVILTLRSAGIDTKQWLHGFDSAEESVKDSIDLIKGHPLI
                     PDGIIVHGLIISPETGKLDVVVNGYEDK"
BASE COUNT         1866 a         1085 c          634 g         2251 t
ORIGIN      
        1 atatacttca atataatcat caactattaa aattaaattc tatttataat aaaatttaaa
       61 gaataaaatt aaatatacat tttgataatc acctccaaca ttttccttac tacatattat
      121 aataaaataa acactttgta aagacataaa attatgtggc tatctcaaaa tatagagaat
      181 cctctttttc attgttaaaa tgataaaaat acactttatt tcatacacaa aagagtgcct
      241 tatgaattaa aaagttcatt ttaaggcacc ctcatttatt ttatcttatt aattacatgt
      301 ctatgctaag atagtttttt gttacattgc ttcatcttct tcaaattgag aattgtaaag
      361 attggcatag aatccgcctt tctttagaag ttcctcgtga tttccttgtt caactatatc
      421 tccttcattc attacaagaa tcatatcagc atcacgtata gttgataatc tgtgggctat
      481 aacaaaactt gttctacctt ccataagatt atccatagct ttttgaatca atacttctgt
      541 tctagtatcg acagaacttg tagcttcatc taatattaaa attttaggat cagctaaaat
      601 agcacgagct atagtcaata attgtttttg accttgagaa acattacttg cttcttcatt
      661 taatttcatc ttatatccat ctggtaatgt cttaataaat ctatgaacat gtgcagattt
      721 agcagcctct ataacttctt catctgtagc attcaactta ccatatctta tattttccat
      781 aattgaacca ctaaataacc atgtatcttg aagaaccata ccaaacattt ctctaagctc
      841 actacgatta aaatctttaa tattatgacc atcaatcagt attgaaccgc tattaacatc
      901 ataaaatctc attaacagtt taactattgt agttttacct gctcctgtag gtccaacaat
      961 agcaactttt tgaccaggtt taacatttac tgagaaatca tttattatag tcttgtttgg
     1021 gttatatcca aagttgacat tttcaaaatc aattttacct tctaaaccat ctattgaaac
     1081 tgcattttca acaatttgga cttcttcttc ctcttctaaa aactcaaata ctctttcaga
     1141 agctgctgct gttgattgca ataaattggc aacctgtgca atctgagtca ttggctgtgt
     1201 aaagtttctt acatattgta taaatgactg tatgtcacca acctcaattg ttttcttaat
     1261 tgcaagccaa cctcctaaga ttgataccat tacatatccc aagtttccaa taaacatcat
     1321 aagtggttgc attatacctg ataaaaattg agatttccac gctgaattat acaggctatc
     1381 atttagttta tcaaactctt caattacttc atcctcacga ttaaatgctt ttactattgt
     1441 ttggccactg taagtttcct caacttgacc atttacattt cctaaatatt cttgttgata
     1501 tctaaagtat ttttgtgatt tctttattat tcttgaaatt acaaacattg atattggtaa
     1561 tattaaaact gccactaatg tcataattcc acttatagat aacatcatta tcaaaacacc
     1621 tatcatagtt gttactgatg ttataagctg tgtcatactt tgatttaaac tttgatttaa
     1681 agtatctata tcattcgtaa ttctcgataa tatttcacca tgcgtcttag tatcaaaata
     1741 cttcattggc aatctatcca attttgctga aatctctttt cttaaattat aggatacttt
     1801 ttgagaaatt ccactcatta taaatccttg tataaaagag aacagtgcac ttatcaaata
     1861 aaggaaaagt aaaaaggtta agatttttcc aattgcatta aaatctatac ctacgtttcc
     1921 tccagaaact ttactcacta gcccttcaaa tatctttgta gttgcctttc ctaaaatttt
     1981 tggacctact atagaaaacg acgcactacc aatagcaaat acaattacta gaattattga
     2041 tattttaaat ttagataaat atgcaattag ttttcgcata gtccctttaa agtcatttgc
     2101 tttttgtcct gctcccatag attttcccat aggacctctt cccattctac tcattagcta
     2161 gctcctcctt tgaaagttgt gataaagcta tttgctcata aacttcgcaa ttttttagaa
     2221 gctctttatg tgttcctatt ccaactattt cgccctcatt taaaacaatt atctgttctg
     2281 catttaagat agtactaatt ctttgtgcaa ctattagaac tgtactttca gcagtttttg
     2341 attttaaagc cttacgaaga gctgcatcag tttttaagtc aagtgctgaa aaactatcat
     2401 caaatatata aacttctgga ttttttgcta ttgctcttgc aatagaaagt ctttgttttt
     2461 gacctccaga tacatttgtt ccaccttgag aaatttcagt tttaaaagct tccggtttag
     2521 aatctataaa ttctgtagct tgagctattt cagctgctgt tctcatttct tcatctgttg
     2581 cgtcttttct accatatttt aagttggatt ctatagtacc agaaaacaat actccctttt
     2641 gaggtacata tccaattttt tcccttaaat catgttgaga aacatttttt atattaactc
     2701 caccaagtaa tatttctcct tcagtaacat caaagaaacg aggaattaaa tttataagag
     2761 ttgacttacc actaccagta cttcctataa aggcagttgt ttctcctggc tttgctgtaa
     2821 aagatatatt tgttaatata ggttcttctg cgtctggata catgaacgaa acatttttaa
     2881 attcaacaag acccttttta tcttccttaa actcttgtgc ctctgatttt tctgggtctt
     2941 ttatgcttat atctgacact aatacttcat ctatacgtgt agctgaaacg gatgctcttg
     3001 gaagcataat agataccatt gatatcatta agaatgacat tattatttgc attgtatatt
     3061 gtataaatgc catcatatct ccaacttgca ttgctccaga atcaacactg tatgcacctc
     3121 tccaaactat aagaactgtg ataaggttca ttataagcat catagcaggc atcatacatg
     3181 tcataactct atttacaaaa agatttgttt tagttaaatt tttatttgct ttatcaaatc
     3241 tttcttcttc atgtttttca gtactaaaag ctctgataac tggaatacca gtaattattt
     3301 cacggcttac taagtttaac ttatcaacaa gcttttgaac cagtttaaac ttaggcataa
     3361 ccactgagaa taacactaca attaatgata agatagcaac tactgctact gctataatcc
     3421 aggccattga agtatttgtg tttaaaacct ttatcactcc acctatagcc attataggtg
     3481 catagaatac tattctaagc aacataacca tcaacatttg tatctgttga atatcatttg
     3541 tactacgagt aattaatgaa gctgtagaaa attcattcat ttcagtattt gaaaatgaca
     3601 taacttttct aaatactcca cttcttaaat ttctacctac acttgcggct actcttgctg
     3661 ctaaaaatcc tactgttatt gtagctacca tactaatcat tgctatacca agcattttag
     3721 caccagtaaa taatatataa ttattttgag ttttttctgt atcaatacct attttctcat
     3781 attctgacct tacatatgaa acagagcttt gtgttatcat actctctggc atactttcaa
     3841 agcctttgct catctcttta gtcaatgtat ctaactgttc ctttggcatt gctgataaca
     3901 atttaaatat atcaacatct ccactttgag tagcttgtgg tggaagtttg ctcattaact
     3961 gagctttcat agcttttacc tctttactat cgccctcaag ccctgatact attaaaatag
     4021 ctttaccaaa aataacattt aaatcatcta tagtgtcttt gtctttagta tttaactcat
     4081 aaagagattc atcttttaga cctggatact tatctttata cttaacataa tcctttttat
     4141 tcagcaaagt ataattatct agtactttat ccttactact tttatccata aataaagtaa
     4201 ttttatttaa ttcactctct ctaatcacag atggaacaga gttttctact ccaccttgtt
     4261 gtatacctac atttactata ttggatgtgt attctggtaa agaaaggtca cataccgctt
     4321 gaattaccaa tagacagact attattacta ttgataatgc cgatttcttt aaatatttaa
     4381 ttaatttaaa catacatttc ttcccttctg taatattaat tagtctaata acattaaatt
     4441 gaaaaacatt attttaaact ttaaaaatat ttttcatctt catgcttatg acatcctgct
     4501 tttaaattat ctcgtacttg cattagaagt cttctaagta ctattttttc ttcttcacta
     4561 aatcctaaaa aaaactctct ttccaattcc ttgttaaatt cttcaacttt attacatatt
     4621 tctaaacctt tgtctgtcaa aaaaatccta gatactcttt ggtccttttc atcttgtttt
     4681 ctttccacaa gtccagcttt ttccatcctc tttatcataa ctgtagttgt agatgctttt
     4741 actttccttt tatctgctaa ttctttttga ctttgcccat ctttcatctt taaagaaaaa
     4801 agtatttggg gttgaccatg atataagcct aatttatcaa aataaaaatg agttcttata
     4861 aaatatattc gcattaattg caagaaaaca tcatttaatt tttcttcaga attgctattc
     4921 atacttgccc tccttatttt ttatattact tagtcggcta agcattattt tactatactg
     4981 catgcatttt ttcaatatat ttataaaaaa ttcatataaa tttcatttat taaatatttt
     5041 tatatcatat agttataatt ttaataaata atagtaagat ttttaattag tcagctactt
     5101 atatttttta ttgtaaagct taaaataaac cttttcatat attttcaaat aaaaaagttg
     5161 tcccaaaata aattttgaaa caacatcata ttcaacagta tcttgattat ataacattat
     5221 gatactctac ttatcttcat atccatttac aacaacgtct aacttcccag tttcaggtga
     5281 tataattaga ccatgtacaa taataccatc tggaattaaa ggatgccctt ttattaaatc
     5341 aatactatct tttacagatt cttctgcaga atcgaatcca tgtaaccatt gttttgtatc
     5401 aattccagca ctacgaagtg taagtattac atcattagat attcctctat ctaaaatttt
     5461 accaagtaac ttatccgtat caacattaca cataccacat ccatgatgcc ctacaaccat
     5521 aacctcatct acatcaaatt cgtaaattgc aactaaaaca cttctcataa tacttccaaa
     5581 aggatgcatt atagtagctc ctgcattttt tattagtttc acatctccat ttttcaaatt
     5641 catagcctta ggcaaaagct cagttaatct tgtatccata caagataaga ctactatttt
     5701 tttatttgga tgttttgatg tcacatattg ttcatattct ttgttctcta caaatgattt
     5761 attatattct aatatctctt caagttttct catatgtaat atcaccctct ttaaaaattt
     5821 ttttattatt atatca
//
LOCUS       DAMJVK010000164         5726 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.169, whole genome shotgun sequence.
ACCESSION   DAMJVK010000164 DAMJVK010000000
VERSION     DAMJVK010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5726
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.169"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            242..424
                     /locus_tag="PC414_003620"
     CDS             242..424
                     /locus_tag="PC414_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465971.1"
                     /translation="MFKDKIDECVHIMTAYIASLKEYYSFIETQIGDFIKKYGEDVVE
                     LCLHRVMILLCECGLA"
     gene            1251..2165
                     /locus_tag="PC414_003621"
     CDS             1251..2165
                     /locus_tag="PC414_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2935 domain-containing protein"
                     /protein_id="HDF2465972.1"
                     /translation="MIDNQKYVILSLELHLFFSRIMKEHALFLEAGFTNKNYNLAMEA
                     DHYKKQFEDLLSYTVSASNGIIRPDILYSEELVTTLTSVAEQKTEEFTGIEINKNITT
                     RELNLQSGVNPQVGQDLVNYVAQLNSDAIRLLDGLINFKERVLDGVLSCTIFTSNYPL
                     LLEHIIHEANLYRSYVVDLENKIDIESKNAKEIELFWDHIMMEHALFMRGLLDPSEGE
                     LINTSNDFAIKFNELIEKTNEMTDSNIKNITEETLNETVEFKDFKEAGASGIEQCKIK
                     SIILPLLADHVLREANHYIRILESYKNM"
     gene            2555..3202
                     /locus_tag="PC414_003622"
     CDS             2555..3202
                     /locus_tag="PC414_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HDF2465973.1"
                     /translation="MIRQTPLEKRYDKLGPHIVTALKKRYFDAYYCKTRNEALQQILD
                     LIPQNHVVSWGGSETLKEMGVQTAVREKGFKVIDRDLAKTPEERTEIMRQALLCDTFL
                     MSSNAISEDGQLFNIDGNGNRVAAMIFGPKNVIVVAGMNKIVKNIDDAIQRTRTIASP
                     AVVQRFQDVNTPCYINGSCIDCTSPESICAYMVTTRISRPAKKIKVILVGENLGL"
     gene            complement(3369..3938)
                     /locus_tag="PC414_003623"
     CDS             complement(3369..3938)
                     /locus_tag="PC414_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465974.1"
                     /translation="MKKFILCIIMFAISVLVLAGCTNDNKIKNEVDSMFNSIKTQDAD
                     TVDKYFPKSGLGNLIYEDKEGFKMYSKNMTWEISDIKNNKDKVTVDLKINNTDMVSIL
                     KKNPPKLGEMMPNPTMKDIDSAKKKEFNITLELVKSGDNYVCKVNQESAMKLLNVITG
                     GGIDYLADYNKDYYKQLDEEQKNYENKNS"
     gene            complement(3943..4842)
                     /locus_tag="PC414_003624"
     CDS             complement(3943..4842)
                     /locus_tag="PC414_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2465975.1"
                     /translation="MKLLSKVNNNIRLITQVAFTALTNGYVNGFLEGTIYGGESKKLC
                     VPGLNCYSCPGALGACPIGSLQAVLSSREYKFSFYVVGFLMAFGAFFGRFVCGWLCPF
                     GLVQDLLHKVPFPVKIKKVYGDKYLKYLKYIVLIIFVIILPVAIVNVAGGGNPWFCKW
                     ICPSGTLLGGIPLILGNENLRESIGFLFSWKLSILLLILLMSIITYRPFCKYICPLGA
                     IYGFFNPISIYRFKIDKDKCTNCTACQTKCKLDIPIYKDVNSPDCIRCGECIKVCPQN
                     AITTTFSKDKKENNICAKKYRGI"
     gene            complement(4853..5002)
                     /locus_tag="PC414_003625"
     CDS             complement(4853..5002)
                     /locus_tag="PC414_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465976.1"
                     /translation="MSNFIKDNKKGISIIAISIIFIALGTYRGETNTVLTKAIMICLE
                     CIGIG"
     gene            complement(4995..5579)
                     /locus_tag="PC414_003626"
     CDS             complement(4995..5579)
                     /locus_tag="PC414_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HDF2465977.1"
                     /translation="MKRFFIVVLGCICVLGMISGCSKKESKPVEKNKNNSEWFSNLDT
                     EDVDGNKVTKEIFSNKELTLINVWTTWCGPCVGEMPELEALSKEYESNNSNVAIKGLV
                     VEVDKTDMRTGLSNEEKNLVKDIMKKSDATYQQLTVSEELKKTDFKRVMEFPTTYFVD
                     KKGNFVGEKVVGANSKEDWKKIIDERLKMVKANE"
BASE COUNT         2097 a          862 c          662 g         2105 t
ORIGIN      
        1 acacttatat taatttatat taaatatcta ctaactcaaa tattcataat taaactttaa
       61 actggataaa ttttctttta agaatgaaag tttatccagt tttgcgatta taataaccag
      121 ttttacatat ccacttgtaa aatatatcta agcaatatat catactatat agatttatga
      181 gtgaaataat atctgctatt tactgaatat ttttattttt cgttttttag ggggattagt
      241 tgtgtttaaa gataagattg atgaatgtgt tcatataatg actgcatata ttgctagttt
      301 aaaagaatac tattcattca tagaaactca aattggtgat tttattaaaa aatatggaga
      361 agatgttgta gagttatgtc tccatagagt gatgattcta ctatgcgaat gtggtttggc
      421 ataaaattaa atttattaaa atgggtaaga agaatcctat ataatattct tcttacccat
      481 tttaatatat caatttcata gcttatcgtt taaaatattt tacatccatt actagacaac
      541 tttacattct ctttttaatt ctagtatatt taaatcattt tagttgctcc ttatttattc
      601 ttattacttg ttttatttat aaaataattc tttttaataa tgttttttgt tgatattcct
      661 atcacacatc caattattta tcattgtctt gaaattgttt gcatacttaa tttaatccaa
      721 ttgaaacacc ccagcataga atcccctgta aaatagaagt tggattaaat cctaacatct
      781 atacagaaaa tactactcca agcacaagca atataatgga atatatttat cacttaattt
      841 tttatacttt ttaaatccca tacctaaaac attaagtgca gctattagaa ttaataattg
      901 ttgtggtata aaattatgct tgcttcatat ttattcattt tttgctataa aagttcttgt
      961 tttacaaaaa acatttgttt tgtatcaaaa taatacttat aaattacata attctcataa
     1021 aaatttctag tattacttgt actcttaata aataatagcc aacaagtacc ttaaatcatt
     1081 atatctatat ctaataagaa acacagtaag ataatctttt cgcccataac ttaataaaaa
     1141 ctgttgatat tagtagcaat tgaatttagt taatttattt ataatataca taaatttaaa
     1201 tattacgaat aaagtattat aaataggata ctttctaagg ggaggaaatt atgatagata
     1261 atcaaaaata tgttatttta tcactagaat tacatttatt tttttcaaga attatgaaag
     1321 agcatgctct ttttttagaa gcaggattca caaataaaaa ttataatctt gctatggaag
     1381 ctgaccacta taaaaagcaa tttgaagatt tattatcata cactgttagt gctagtaatg
     1441 gtataattag acctgatata ttatattcag aagagcttgt aactactctc acatcagttg
     1501 cagaacaaaa aacagaagag tttacaggga tagaaataaa caaaaacatc actacaagag
     1561 aattaaatct acaaagtggt gtaaacccac aagttggtca agacttagtg aactatgtag
     1621 ctcaacttaa ctctgatgca ataagattac ttgatgggct tattaatttt aaagaaagag
     1681 tcttagatgg tgtactatca tgtactatat ttacatcaaa ctaccctcta cttcttgaac
     1741 atataataca tgaagcaaat ttatatcgtt cttatgtagt tgaccttgaa aataaaatag
     1801 atattgagtc aaaaaacgct aaagaaatag aattattctg ggaccatatt atgatggaac
     1861 atgctctgtt tatgagagga ttactagacc cctcagaagg tgaactaata aatacttcaa
     1921 atgattttgc tataaaattt aatgaattaa ttgaaaaaac aaacgaaatg actgattcta
     1981 atatcaagaa cattacagaa gaaactctaa atgaaactgt tgagtttaaa gattttaaag
     2041 aagcaggagc atcaggaata gaacagtgta agataaaatc tataatatta ccacttttag
     2101 cagaccatgt tttaagagag gcaaatcatt atattagaat attggagagt tataaaaaca
     2161 tgtaattaaa aattactttt aaaataatct ttgcatattt agataaaata aaggtacagt
     2221 taactgtacc tttatttttt ataaacttat aaaatttaaa caagttattc ataaaataaa
     2281 ataataatca tctataaccc acaactttat tttatattaa ataaaaagta ttagtggtta
     2341 tgttttataa gtatttcttt attcagttat ttattaaaaa ctaatagtaa atcttatact
     2401 aatcgcatat ttatgttata actatattgt aactattttc gtaatcttag gaaaagatta
     2461 cttataaaat taaccttata caataatata tcgtataatc ttaattagca taatagttac
     2521 aatattaaaa tttaaatatg ggagtgattt tagtatgata agacaaacac cattagagaa
     2581 acgatatgac aagctaggtc ctcatattgt aacagcactc aaaaaacgtt attttgatgc
     2641 ttattactgt aaaactagaa atgaagcttt gcaacaaatt ttagatttaa ttcctcaaaa
     2701 tcatgtagta tcatggggag gatctgaaac acttaaagaa atgggcgtac aaactgctgt
     2761 tagagagaaa ggatttaaag ttattgatag agatttagca aaaactccag aagaaagaac
     2821 tgaaattatg cgtcaagcac ttctatgcga tacttttttg atgagtagca atgcaattag
     2881 tgaagatgga caactcttta atattgatgg aaatggcaat cgtgtagctg caatgatatt
     2941 tggtccaaaa aatgttatag ttgtagctgg tatgaataaa attgtaaaaa acattgatga
     3001 tgcaatacaa agaactagaa caattgcatc tccagcagtt gttcagcgtt ttcaagatgt
     3061 caatacacca tgctatatta atggaagttg tatagattgc acaagtcctg aaagcatttg
     3121 tgcatacatg gttactacaa gaatcagtcg tccagcaaaa aaaataaagg taatattggt
     3181 aggagaaaat cttggtctat aaaatataca tacatgtaaa tagctctata aataataaaa
     3241 tcatatgaaa taacttattt ctaaaagtgg tagaataaaa gcttctacaa gactttcatt
     3301 ctgccacttt tatattcttt taatttgtgt ttctatatta ttaattgctc caattttcat
     3361 atatattact aactattttt attttcataa tttttttgtt cttcatcaag ctgtttataa
     3421 taatctttat tatagtcagc tagataatct ataccacctc ctgtaatcac atttaataat
     3481 ttcattgcag attcttgatt aactttacaa acataattat caccagattt aacaagctct
     3541 aatgtaatat taaattcttt tttctttgca gaatcaatat ctttcatagt tggatttggc
     3601 atcatttctc ctagtttagg aggattcttt tttaaaatag aaaccatatc agtattgtta
     3661 atttttaaat ctacagtcac tttatcctta ttatttttta tatctgaaat ttcccaagtc
     3721 atatttttag aatacatttt aaatccttct ttatcttcgt atataagatt acctaaccct
     3781 gattttggga aatacttatc aacagtatct gcatcttgtg tttttattga attaaacata
     3841 ctatccactt cattttttat cttattatca ttagtacaac cagctaacac cagtactgat
     3901 attgcaaaca ttataataca tagaataaat ttcttcatta aattatatcc ccctatactt
     3961 ttttgcacaa atattattct cttttttatc ttttgaaaaa gttgtagtaa ttgcattttg
     4021 tggacaaact tttatacatt caccacacct aatacagtct ggactattta catctttata
     4081 tattggtata tcaagcttac attttgtttg acatgctgta caatttgtac atttatcttt
     4141 atcaatttta aatctatata ttgaaatagg attaaaaaaa ccatagattg ctcctaaagg
     4201 gcatatatac ttgcaaaatg gcctataagt aatgattgac atcaataata tcaataacaa
     4261 aatacttaac ttccagctaa ataaaaatcc tattgattct cttaaattct catttcctaa
     4321 aataagtggt attcctccca aaagtgttcc tgaagggcaa atccatttac aaaaccaagg
     4381 atttccaccc cctgctacat ttacaatagc aactggcaaa ataattacaa atattattaa
     4441 gactatatac tttagatatt ttagatattt atcaccataa acttttttta tctttactgg
     4501 aaagggtact ttatgtagta aatcttgtac aagtccaaag ggacaaagcc aaccacaaac
     4561 aaatctgcca aaaaaagcac caaatgccat taaaaaacca acaacataaa atgagaattt
     4621 atactctctg cttgataaaa ctgcttgtaa tgaacctatt ggacatgctc caagtgcacc
     4681 tggacatgaa taacaattca atccaggtac acataatttt ttactttctc ctccatatat
     4741 agttccttct aaaaatccat ttacatatcc atttgttaat gctgtaaatg caacttgagt
     4801 aataagtctt atattattat ttactttaga taataacttc attcctcttc tcctaaccaa
     4861 taccaataca ctcaagacaa atcataattg ctttagttaa tactgtattt gtttctccac
     4921 gatatgtacc taatgcaata aatataatac taattgcaat aatagaaatg ccttttttat
     4981 tatctttaat aaagttactc attggctttt accattttta gtctttcatc tataattttt
     5041 ttccaatctt ccttactatt tgcacctaca accttctcac ccacaaagtt tcctttcttg
     5101 tctacaaaat aagttgttgg aaactccata actcttttaa aatcagtttt tttaagttct
     5161 tcagatacag tcaattgttg gtaagttgca tctgactttt tcataatgtc tttaactaaa
     5221 tttttctctt catttgaaag accagttctc atatcagttt tatcaacttc tacaactaat
     5281 ccctttatag ctacattgga attattactc tcatattctt ttgataatgc ttcaagctct
     5341 ggcatttcac ccacacatgg tccacaccaa gtagtccaga cattaataag agtaagttct
     5401 ttatttgaaa atatctcttt tgttacctta tttccatcaa catcctccgt atctaaatta
     5461 ctaaaccatt cactattatt cttgtttttt tctactggct tagattcttt tttgctacat
     5521 ccagaaatca tacctaaaac acaaatacat cctaaaacta ctatgaaaaa ccttttcata
     5581 tctcacactc ctagaattta tatagtatat aaaatcgctg cttgaaataa aaataactta
     5641 tcttctatat taaagtaatt atatttaata aataagagct aatttaaaaa aagatataca
     5701 gttaaaatct atttcttttc agacaa
//
LOCUS       DAMJVK010000165         5625 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.170, whole genome shotgun sequence.
ACCESSION   DAMJVK010000165 DAMJVK010000000
VERSION     DAMJVK010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5625
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.170"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            240..1067
                     /locus_tag="PC414_003627"
     CDS             240..1067
                     /locus_tag="PC414_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HDF2465978.1"
                     /translation="MDLSKKVKILENASDGKSEFEILEFEKLEGASSPLMAMGLYFAR
                     EAGLKARQIKIKLNNSSIKTEAGALYYYKGNISSETKIGGVGGLFKKGISGALTNESM
                     MKPVYKGTGEVYLEPSFRHYLFMSLDNDSIIVDKGLFYCCSDTIEVKAFTQKNFSSAL
                     LGGEGFFQIELSGTGVIVLECVVPQSEIVEYELKNGEELKVDGNFAIARTSGVNFSVT
                     KSDKSLIGSALNGEGFLNTFTGNGTVWLAPTQVMYEKLSFGAMPTNDNMNNKVSKQK"
     gene            2092..2556
                     /locus_tag="PC414_003628"
     CDS             2092..2556
                     /locus_tag="PC414_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2465979.1"
                     /translation="MNLNKVKFTEEHAIEVTNWKYEGKYSIYNLPPWEEIKKKNFSLA
                     KEDKRKNFISFINDNKELIGFINLLDEGSSVFFGIGMKPNYCGMGIGKEIIGLGLQEC
                     RNKYSTKPIVLNVRTWNMRAVKCYESQGFKIVETKVQKTHLGDGEFFVMRYQ"
     gene            complement(2823..3467)
                     /locus_tag="PC414_003629"
     CDS             complement(2823..3467)
                     /locus_tag="PC414_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2465980.1"
                     /translation="MFGQSFQNCLVLIKENFKLSTLTNLICSIILLCMVPLIQGVANL
                     DRISTAICLENYVAIIGIVMLVPVFSLEQSKEIDEIVSSKALSQVIVQLIRILMSIIG
                     IVVLIIGFVNVLIYLGCNFPVVEYIIGSFASAMFIGSLGMFFSNLFNNTIIGYMISIG
                     YLILNMMTKGKYVGKFFIMSMSQGSFDEKYWLILGSVVLIIASLLIKPVKRKIR"
     gene            complement(3397..4686)
                     /locus_tag="PC414_003630"
     CDS             complement(3397..4686)
                     /locus_tag="PC414_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2465981.1"
                     /translation="MNLFIKECKNILKSMVYYIFIVVLVFFYISQLGSSVSNDVSKNI
                     PNDGNPLVAPQKVENIEEYYSVHNQYPYGQKESMKTEKVIPNATINLIREYQKNTYTT
                     YPTGVVKYIKLDQNKTKTIEKYIEEITGKSISEINSLWKTDESIKIDYSKVSLERFEE
                     IMQRVDDILGGGSAYAFDKLQRFGVVPVTYDEAMKNYNYTIQKDKVSNGYARIFCDYI
                     GIVLSIFPVFVAVFMSLKDKRSRMREIIYSKQVSSCKIVLSRYFALVFMMILPVLILG
                     MKDLLPLIFFAKDKGISIDNLAFIKYTLWWILPTLMIVTAVGMSITILTDTPVAIIVQ
                     LIWWLYSINSTGLKGDYSTMGLMIRHNSVLNGDIIIQNFNQIMINRIVISLGALVLVL
                     LAIFLYEQKRRGNLDVWAKFSKLFSSNKRKLQIKHFN"
     gene            complement(4683..5555)
                     /locus_tag="PC414_003631"
     CDS             complement(4683..5555)
                     /locus_tag="PC414_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2465982.1"
                     /translation="MEMKIAVSNLSKRFGKKQALDNINLEIDSGMFGLLGRNGAGKTT
                     FMRILATLLSKTEGSIHMCGVPIENTKEIRNMVGYLPQDFSMYPNMTVYKAMDYLAVL
                     SDIPLRKRRKIIAKLLSKVNLTNYQNVKVKSLSGGMNRRLGIAQALIHNPRVLIVDEP
                     TAGLDPEERIRFRNLLCEIANDRIVILSTHIVGDIEATCENVAILNSGKVIYNGSTEA
                     LAKLAEGKVYSIEVDKKDIENIKSRFIVIGMLTHGGKAILRIISDDKPFETAVNCNPT
                     IEDGYMLIMGGDNI"
BASE COUNT         2037 a          778 c          699 g         2111 t
ORIGIN      
        1 ataattttaa catgcccata aaatataata cccacaaatg ataaataata ctcaaaatgt
       61 ctattgaaat aactatacat ccttaaataa cttaacttaa atctaaacat ttcaaaaact
      121 tcttaaaaat atgtaaaaat ttgatgataa agtagcgatt ttttcaaaaa aaatgtaata
      181 tagagttata tcttataata ttttagagat aaattttatc atagtgggga gaaatttgta
      241 tggatttaag caaaaaagta aaaattttag aaaatgcatc agatggcaaa agcgaatttg
      301 agatattaga atttgaaaaa cttgagggag cttctagccc attaatggca atgggattat
      361 attttgctag agaagctggt ttgaaagcta gacaaattaa gataaaatta aataattcat
      421 caataaaaac agaagctggt gcattatact attataaagg aaatatatct tctgaaacta
      481 agattggtgg tgttggaggt ctttttaaaa aaggaatatc aggagcactt actaatgaat
      541 caatgatgaa acctgtatac aaaggtactg gagaagttta tttggaacct tcttttagac
      601 attatttatt tatgagtctt gataatgatt ctataattgt tgataaagga ttattctatt
      661 gttgctctga tacaatagaa gtaaaagcat ttacacaaaa gaacttctca tctgctttac
      721 ttggtggtga aggcttcttc caaatagaat taagtggtac tggtgtaatc gttctggaat
      781 gtgttgtacc tcaaagtgaa attgtagagt atgaattgaa aaatggtgaa gaattaaagg
      841 tagatggtaa ctttgcaata gcaagaacat ctggtgttaa tttttctgtt acaaaatcag
      901 ataaatcttt aattggttct gctttaaatg gtgaaggttt cttaaataca tttactggta
      961 atggtacagt ttggctagct ccaactcaag taatgtatga aaaactaagt tttggagcaa
     1021 tgcctactaa tgataatatg aataataaag tttctaagca aaaatagttg ttttataaat
     1081 atttattaaa tcattattga ttttataaag tcactataaa ttttattaga ttattattat
     1141 aaaatcagag tgtggaaatg tttatgtgta ttctttaaat aaaaatttcc acattctgta
     1201 aggttattat acttgatata taaaattcaa tttaggaaca tagtctaaca tttataaaca
     1261 tattaaggta atgatttctc ttggtaatta ttctcatatc ttattctttg attatattta
     1321 tcttattcct agcccttatg tcatattata tttcttatct tattacttat aattagcatt
     1381 aacttatcta aaatcttgta tttttttaat agtaaagttt acatctatat taaaaagttt
     1441 acatctatat taatttcata aaacaatcaa ggtagtcaag agattcaact ttacttgatt
     1501 ttaaaaattt aagtgaattt gttcttatac acatcttttt aaaattatat ctgtagaaaa
     1561 tatcatttag agctataatt aaattaactt attaaatttt agaaaattta ttaaaaaata
     1621 taagttaaat taatattttt aaagattctt ttttgtaact actagaatag taatttagta
     1681 gttattgtat ctctaaagtt taagcatgga tagatataca tttgataatt tgcctataat
     1741 ttacctacta cttggattat tggagcaaat acaatatatt aataataagt tcctaaaacc
     1801 aataaattaa ctaatacaca taaagatatt gatgttgatt atacaactat aagaaacgga
     1861 tttaacagaa tttaatcaag ctaagaaatt atatatatat cgtgatgttt ttgaattaga
     1921 aggtaattca aaattagttt atagtcttct gtcaatccaa taaaaagtac agggtacaag
     1981 aattgttagt aaaattttta aagattttat ggataaatat aaatagtttt acttattttc
     2041 taaaacttta aaataccatt aattacctta aatattttaa taggaggaca tatgaattta
     2101 aataaagtta aatttactga agaacatgct attgaagtta ctaattggaa atatgaaggt
     2161 aaatattcaa tatacaatct acctccatgg gaggaaatta aaaagaagaa tttttcacta
     2221 gcaaaagaag ataaaagaaa aaattttata agttttataa atgacaataa agaattgata
     2281 ggttttataa acttattaga tgaaggaagc tctgtattct ttggtattgg tatgaaaccg
     2341 aattattgtg gaatgggaat tggtaaagaa ataattggcc taggattaca agaatgtaga
     2401 aataaatata gtactaaacc aattgtatta aatgtaagaa cctggaacat gagagctgta
     2461 aaatgttatg agtctcaagg atttaaaata gttgagacta aggtacaaaa aacgcattta
     2521 ggagatggag aattttttgt tatgaggtat caatagttta taacaatagt cttattgtta
     2581 atacaacaat taaaatgaaa aaatacatat gtgtaggtca tagcaataag tgtctttgaa
     2641 atataaattt gagagctttt gataaaattt tgtatacata tatattttat actacggata
     2701 tataaatcta tctacatata taaagagatt gtaagtaggt ttgtgtatat aaaaatagat
     2761 ttatatttca taaatgtact ttttatttgt attaccttta ttttatctaa gctctcattt
     2821 tattatctaa tcttcctttt tactggtttt attaagagag atgcaattat caacacaaca
     2881 cttccaagta tcagccaata tttctcatca aaactacctt gactcataga cattataaaa
     2941 aattttccaa catacttgcc tttagtcatc atatttaaaa ttaaatatcc tatagatatc
     3001 atataaccta tgattgtatt attaaataaa tttgagaaaa acattcctaa acttcctata
     3061 aacatagctg atgcgaaact tccaataata tactcaacta ctggaaaatt acatccaaga
     3121 tatattaaaa catttacaaa acctatgatt aataccacta tacctataat tgacatcagt
     3181 atcctaatta attgtacaat aacttgagat aatgcttttg aagatactat ctcatcaatt
     3241 tcttttgatt gctctagcga aaatacaggt accaacatta ctatccctat tattgcaaca
     3301 taattttcta aacatatagc tgttgaaatt ctgtctaaat tagctactcc ttgtattaga
     3361 ggaaccatac atagcaatat aattgaacat attaaattag ttaaagtgct taatttgaag
     3421 ttttctttta ttagaactaa acagttttga aaactttgcc caaacatcaa gattccccct
     3481 tcttttttgc tcatataaga atatagctaa caaaacaagc accaatgctc ccaagcttat
     3541 cacaattcta ttaatcataa tttgattgaa gttttgtata ataatatccc catttaatac
     3601 actattatgt cttatcatta gccccattgt tgaataatca cctttgagtc ctgtagaatt
     3661 tatactgtaa agccaccaaa ttaattgcac aataattgct actggtgtat ctgttaatat
     3721 agtaatactc attcctacag ctgtaactat cataagagtt ggtagtatcc accataatgt
     3781 atattttata aatgctaagt tatcaataga tataccttta tctttagcaa agaaaatcaa
     3841 aggaagtaaa tctttcatac ctaaaattaa tacaggcaat atcatcataa agacaagtgc
     3901 aaaatatctt gataaaacta ttttgcatga agatacttgt tttgaataaa taatctctct
     3961 cattcttgaa cgcttatctt taagtgacat aaaaactgcc acaaatactg gaaatatact
     4021 taaaactata cctatataat cgcaaaaaat tcttgcatac ccattactaa ctttatcttt
     4081 ttgtattgta tagttgtaat tcttcatagc ttcatcatag gtaactggaa ctactccaaa
     4141 tctttgtaac ttgtcaaaag catatgcaga ccctcctcct aaaatgtcgt ctactctttg
     4201 cattatttct tcaaatcttt caagagatac tttagaataa tctattttta tactttcatc
     4261 agttttccaa agtgaattta tttcgctaat acttttacca gttatttctt ctatatattt
     4321 ttctatggtc ttcgttttat tttggtctaa ttttatatac ttaactaccc ctgttggata
     4381 cgttgtatat gtgttttttt gatattctct aattaggttt atagttgcat ttggaatgac
     4441 cttttctgtt ttcatagatt ctttttgacc atatggatat tgattgtgta cagaataata
     4501 ttcctcaata ttctctactt tttgtggagc aactaatgga tttccatcat taggaatatt
     4561 tttacttaca tcatttgaaa ctgaacttcc aagttgagaa atatagaaaa aaactaaaac
     4621 tacaataaaa atataatata ccatactctt taatatattt ttacattctt ttataaatag
     4681 attcatatat tatctcctcc cattataagc atatagccat cttcaatagt tggattacaa
     4741 tttactgctg tctcaaaagg tttatcatct gatataatcc taagtatagc ctttccacca
     4801 tgagtcaaca ttcctataac tataaatcta ctttttatat tttcaatgtc ttttttatct
     4861 acttcaatac tatatacttt cccttcagct agttttgcta atgcttctgt tgagccattg
     4921 tatatgactt ttccactatt caatatagct acgttttcac atgtagcttc tatatctcca
     4981 acaatatgtg tagataaaat aacgattcta tcatttgcaa tttcgcatag taaatttcta
     5041 aatcttatac gttcttctgg gtcaagacct gctgtcggct catctactat taaaactctg
     5101 ggattatgaa ttaatgcttg tgctatccca agccttctat tcatacctcc agataaagat
     5161 tttactttca cattttgata attagtaagg tttactttgc ttaataactt tgctataatt
     5221 tttcttcttt ttcttagtgg tatatccgat aaaactgcta aataatccat tgctttatat
     5281 actgtcatgt taggatacat tgaaaaatct tgtggcaaat aaccaaccat atttctaatt
     5341 tccttagtat tctcaatagg aactccacac atatgtattg aaccttctgt ttttgataat
     5401 aaagtggcta aaattctcat aaatgtggtt tttcctgctc catttcttcc taaaagacca
     5461 aacataccac tgtcgatttc aagatttata ttatcaagag cttgtttttt cccaaatctt
     5521 ttactaagat tggatactgc tattttcatt tccatatata aatacacccc cagaaacaat
     5581 tttatagaat tttattttta taaattttta ctgtattttc ataat
//
LOCUS       DAMJVK010000166         5533 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.171, whole genome shotgun sequence.
ACCESSION   DAMJVK010000166 DAMJVK010000000
VERSION     DAMJVK010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5533
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.171"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            55..171
                     /gene="rrf"
                     /locus_tag="PC414_003632"
     rRNA            55..171
                     /gene="rrf"
                     /locus_tag="PC414_003632"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(385..1569)
                     /locus_tag="PC414_003633"
     CDS             complement(385..1569)
                     /locus_tag="PC414_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2465983.1"
                     /translation="MLSKRLNFITPSYTIGISSKVKEMESNGVKVINLSIGEPDFNVP
                     NNAKSYGIDSLNKDYTKYDLVPGLKILREEICKKLIEENNCNYSIDEIVVSSGAKNSI
                     TNTLLALTDEGDEVLLPKPYWVSYPEMVKLVNAVPVFIDTKKENGFKLTKDELEKSIT
                     DKTKILVINNPSNPTGSVYTKDELIEIVDVCIQNKIYILADEIYEKICYTGEFTSIAS
                     LSEEAKDITITINGFSKSAAMTGLRLGYTASNKTIAKAMSSIQGHLISHPSLTAQYIA
                     YGALKDCSIDIDNMVKTYKSRRDLIKSKLDSIDNVGYVNPNGAFYIFIDLSKVSEKFE
                     YKDSFSIEFCNQFLEEYNVAVVPGIAFGMDKYIRISYACSENTFLSGLDKLKEFVYKI
                     MA"
     gene            1942..4293
                     /locus_tag="PC414_003634"
     CDS             1942..4293
                     /locus_tag="PC414_003634"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside triphosphate reductase"
                     /protein_id="HDF2465984.1"
                     /translation="MVEFVKKRDGRVIPFNEDRITRAIFLAATNVAEREGIVPDYKLS
                     EQLTQEVIKFLNHKYSESVPSVEDIQDSVVKVLIETGHAKTSEEYIIYRTERSRIRNS
                     KTRLMKAIEEITFEDAEDADIKRENANINGNTAMGTMLQYGSTVSKEFCKTHILKPEH
                     SFAHDNGDIHIHDMDFLNMGTLTCCQIDVKKLFNGGFSTGHGFLREPQDIISYGALAA
                     IAIQSNQNDQHGGQSIPFFDYGLAEGVYKTFKKFYIGNLAKALKLFKGIENNDVIKNI
                     VYNTEKETNQKVGLKRDELYLNLEKEKLIQTFDIDDELVNKMQNFAFEESYRETDKKT
                     YQSMEAFIHNLNTMHSRAGAQVPFSSVNFGTDTSEEGRMVTKNLLLSQERGLGNGETP
                     IFPILIFKVKEGINLNPEDPNYDLFKLSCRVSAKRLFPNFSFLDAPFNAKYYKKGEPD
                     TEATYMGCRTRVLSNVCGSETVSGRGNISFTTVNLPRLGIKHGIINNEKANLDGFFEE
                     LDEKINLIIEQLLERLEVQGNKKMKNFPFLMGQGVWKGSDDLGPEDTLKEVIKQGTLT
                     IGFIGLAECLIALIGKHHGESKEAQELGLKIVSHMRHKMDEATDKYKLNFSLMGTPAE
                     GLSGRFTKIDKKVYGEIKGITDKEYYTNSFHVPVYYNISAYDKIEIEAPYHELTNAGH
                     ITYVELDGDPSDNLEAFETVIKAMKDLGIGYGSINHPVDRDPICGFSGVITSNICPVC
                     GRNEDESDIKFERIRRITGYLVGTVDRFNNAKKAEVRDRVKHR"
     gene            4315..4854
                     /gene="nrdG"
                     /locus_tag="PC414_003635"
     CDS             4315..4854
                     /gene="nrdG"
                     /locus_tag="PC414_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HDF2465985.1"
                     /translation="MKIRMSSTISYDSIVDGPGLRMVIWTQGCIHNCKECHNPQTHDL
                     CGGFYMDTEEIINKVKSLKLQKGITLSGGEPFLQPEPLEEIAREAKINGLDVWSYTGF
                     TFEQLLDKKNRAYFKNLNLLKQIDILVDGKFIAEKKDISLKFRGSSNQRIIDVQKSLK
                     YKKVFLVEQYMKDDLSIAE"
BASE COUNT         2156 a          628 c          891 g         1858 t
ORIGIN      
        1 ctaggttcta taaacttatt aaaaacttaa tagaaatatt aagcataaag attatgtggt
       61 tacaatagca gagaggatac acctgttccc attccgaaca cagaagttaa gctctctagc
      121 gctgatggta cttggtggga aactgcctgg gagagtagga cgtagccacg taattttttt
      181 gttataataa atttagaatt taacttatat agtttaattt gataatttga tgaaaaaaat
      241 aaaagactta ctgattttta aatctatagg tcttttattt tttatctaac atttaaatta
      301 agttgttgaa tatatcttat attacttacc ttatatatta attaccatta atacattata
      361 tctaattact attcatctaa attattatgc cattatttta tatacaaatt cttttaattt
      421 atctaatcca gataagaaag tattttcact gcaagcatag gatatacgaa tatatttatc
      481 cataccaaat gctatgccag gaacaactgc aacattatat tcttctaaaa attgattaca
      541 aaattctata gaaaaactat ctttatattc aaatttctca gatactttag aaagatctat
      601 aaatatatag aatgcgccat ttggattcac atatcctaca ttatcaatag agtctagttt
      661 tgatttaatt aaatctcttc ttgatttata tgttttaacc atattgtcta tgtcaattga
      721 gcaatctttc aaggctccat aggctatgta ttgtgctgtt agacttgggt gagatataag
      781 atgtccttgt atagaactca ttgctttagc aatcgtttta ttagatgcag tatatcctaa
      841 tcttaatcca gtcattgcag ctgacttaga aaaaccattt atagttattg ttatatcttt
      901 agcttcttcg cttaatgatg caattgatgt aaattcacca gtgtagcata ttttttcata
      961 tatttcatct gctaaaatat atattttatt ttgtatgcat acatctacta tttctattaa
     1021 ttcatctttt gtataaacac taccagtagg atttgaagga ttgtttatta caagaatttt
     1081 tgttttatct gttatagatt tttcaagttc atcttttgtt aacttaaatc cattttcttt
     1141 tttagtatca ataaaaactg gaacagcatt aactaactta accatttcag gataactaac
     1201 ccaataaggc tttggtaata agacttcatc tccttcgtct gttaaagcta gtaaagtatt
     1261 tgttattgag ttttttgctc cacttgatac aactatttca tctattgaat agttacagtt
     1321 attttcttca ataagttttt tacagatttc ctctctaagt atttttaatc ctggaactaa
     1381 atcatattta gtgtaatctt tatttaaaga atcaatacca taagattttg cgttattagg
     1441 tacattaaaa tcgggttctc ctatactaag atttattact ttaactccat tactttccat
     1501 ttctttaact ttagaactaa tacctattgt gtaggatggt gttataaagt ttagtctttt
     1561 tgataacata aaatgcctcc ttttaaatta ttatttatat tatacgacaa atttaaagaa
     1621 tttacataag gaaatttgta gatatataca ttaatatata taatagccta cactttttgc
     1681 ctaaaattag tgtaaaaaaa agagaaaaaa attttaactt tcaaagttgt tagaaacgct
     1741 actgttcaaa atatccacat agtatttgca atgatttgcg caagtttgac tattatgtac
     1801 aaatttggta atcttaatat agcaattatt aagataatcc ctttaactta gttgaagggg
     1861 atttttttag acttaacata ctacatatag tggtatgggg attaataaaa tacaatatat
     1921 agtaacagga ggttttttta attggttgaa ttcgtaaaaa aaagagatgg aagagtaatt
     1981 ccatttaatg aagatagaat aacaagagct atatttttag cagcaacgaa tgttgcagaa
     2041 agagaaggaa tagtacctga ttataaatta tctgagcaat taactcaaga agtaataaag
     2101 tttttaaatc ataaatactc agaatcagtt ccaagtgttg aagatataca agattctgta
     2161 gttaaggttc ttatagaaac tggccatgca aaaactagtg aagaatatat aatatacaga
     2221 actgaaagaa gtagaataag aaattcaaaa acaagattga tgaaggcaat agaggaaatt
     2281 acctttgaag atgcagaaga tgctgatata aaaagagaaa atgcaaatat taatggtaat
     2341 acagcaatgg gaactatgtt acaatatgga agcactgtat ctaaagaatt ttgtaaaaca
     2401 catatattaa aaccagagca ttcttttgct catgataatg gagatataca catacatgat
     2461 atggattttt taaatatggg aacattaact tgttgtcaaa tagatgttaa aaaactattc
     2521 aatggtggat tttcaacagg acatggcttt ttaagagagc cacaagatat aataagttat
     2581 ggtgcattag ctgcaattgc tatacaaagc aatcaaaacg atcaacatgg aggtcaaagt
     2641 ataccatttt tcgattatgg attggctgaa ggtgtatata agacatttaa gaaattctac
     2701 ataggaaatt tagctaaagc attgaagtta tttaaaggaa ttgaaaataa tgatgtgata
     2761 aagaacatag tatataatac agagaaagaa acaaatcaaa aagttggtct taagagagat
     2821 gaattgtatc taaacttaga aaaagagaaa ttaatacaga cttttgatat agatgatgaa
     2881 cttgtaaata agatgcaaaa ctttgcattt gaagaatcgt atagagaaac tgataaaaaa
     2941 acatatcaat ctatggaagc atttatacac aatctaaata caatgcactc tagagcaggt
     3001 gcacaggtac cattttcaag tgtaaacttc ggaacagata cttctgaaga aggaagaatg
     3061 gtaactaaga atcttctatt atctcaagaa agaggtttag gtaatggaga gacaccaatt
     3121 tttccaattt taatatttaa ggtcaaagaa ggcataaatt taaatcctga agaccctaat
     3181 tatgatttat ttaaattatc atgcagagta tcagctaaaa gactattccc taattttagc
     3241 tttttagatg cgccttttaa tgcaaaatat tataaaaaag gtgaaccaga tacagaggct
     3301 acatatatgg gttgtagaac aagagtactt agtaatgtgt gtggttcaga aacagttagt
     3361 ggtagaggaa acatatcttt tactacagta aatttaccaa gattgggtat aaaacatggt
     3421 attataaata atgaaaaggc taatttagat ggtttctttg aagaattaga tgagaagata
     3481 aacttaatta tagaacaatt attggagcgc cttgaggtac aaggaaataa aaagatgaag
     3541 aattttccat tcttaatggg gcaaggtgta tggaaaggtt ctgatgattt aggaccagaa
     3601 gatacactaa aagaagtaat aaaacaagga acattgacaa ttggatttat aggtttagca
     3661 gagtgcctta ttgctcttat aggtaaacat catggagaaa gtaaagaagc tcaagaatta
     3721 ggattaaaaa tagtgtcaca tatgagacat aaaatggatg aagcaacgga caaatataaa
     3781 ttaaatttct ccttaatggg tactccagca gaaggtcttt caggtaggtt tactaaaata
     3841 gataaaaaag tttatggaga aattaaaggt atcacagata aagaatatta tactaattca
     3901 ttccatgtac cagtttatta caatataagt gcatatgata aaattgagat agaagctcca
     3961 taccatgaat tgactaatgc aggtcatata acatatgtag agttagatgg agatccatct
     4021 gataatttag aagcttttga aactgttata aaagctatga aagatttggg tataggatat
     4081 ggaagtataa atcatcctgt agatagagac ccaatatgcg gtttttcagg tgtaataaca
     4141 agtaatatat gccctgtatg tggaagaaat gaagatgaaa gtgacatcaa attcgaaaga
     4201 ataagaagaa taacaggata cttagttggt acagttgata gatttaataa tgctaaaaaa
     4261 gcagaagtaa gagatagagt aaaacataga tagttttatt tgggggaata agttatgaaa
     4321 ataagaatgt catctactat aagttatgac agcatagttg atggaccagg attaagaatg
     4381 gtaatatgga ctcaaggatg tattcataat tgtaaagagt gccataatcc tcagacacat
     4441 gatttatgtg gaggatttta tatggataca gaagaaatta taaataaagt caaatcattg
     4501 aaattacaaa aaggcattac gctatcagga ggagaaccat ttttacaacc agaaccatta
     4561 gaagaaatag caagagaagc taaaataaat gggttagatg tatggtcata tactggattt
     4621 acgtttgaac aactgttaga taaaaaaaat agagcgtatt ttaaaaattt aaatttacta
     4681 aagcaaattg atattttagt ggatggaaaa tttatagctg aaaaaaaaga tataagctta
     4741 aagtttagag ggtcatcaaa tcaaaggata attgatgttc aaaaaagttt gaaatataaa
     4801 aaagtgtttt tggttgagca atatatgaag gatgatttat ctatagcaga ataaaatata
     4861 aataaacagg attaaatata tgcatatatt taatcctgtt tattttcata taatatttca
     4921 agcttgtata atgtaacaat taaaccaaaa gactcattgt atatttgaaa ttaagttttt
     4981 tatagtttat aggtgtttta tgtggattac gaatgatata gtattgatac attatttaat
     5041 agattcttat gccaagaaac aattaattta tagaaattct taattgttac aatataaata
     5101 tctagataag ttataagata ctgataaata atttataaag tttattaatc taatattgtg
     5161 tgtgaatatt aagtggtcaa gtagtctaaa aaatgaaaaa gatttaaaaa agtgttttaa
     5221 ctaaacacat aagtgttaaa ttcatgtata agtataggtt agatacataa gaaataatta
     5281 tgtaatatct atctttgtaa aaaaatatta aattttgtaa tttgtggata aatatgttta
     5341 taatgtggat aatatttaat gtaataagtt aaagcaatat gaatcaactg tttataatgt
     5401 ggataatatt ataaatgtat gaataattgc agtatttaaa aacaaaaaaa gttaaaaaag
     5461 ttgttgactc taaaagaaaa agttggtata gtaatacttg tcctcaagaa aagggcaaac
     5521 aaataaagca taa
//
LOCUS       DAMJVK010000167         5509 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.172, whole genome shotgun sequence.
ACCESSION   DAMJVK010000167 DAMJVK010000000
VERSION     DAMJVK010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5509
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.172"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            207..1412
                     /locus_tag="PC414_003636"
     CDS             207..1412
                     /locus_tag="PC414_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDF2465986.1"
                     /translation="MSKKVLIMSASTGGGHNRAALAIKEELTSKTLDGEPIECEIIDS
                     LKLVNNTMDKIISRGYEKSAIYTPKAYGSVYRLSETNLLSKNEFKDNLLITFMAKKFK
                     KLIRSEKPDLIIGTHPFPMIALSTLKKNFNLHNNESNAYTEHFYKHYTNTINVPPLIS
                     VLTDYTTHSTWIQNEIDYYIVGHEYVKELLVFDGVEPSKIRTFGIPVEKSFLSHRDKG
                     IVLSELNLSPDKLTVLLMGGSFGAGNIKETLDELLDTDRDFQILVITGKNESLKEKIE
                     KKLMSRYHDKNVCVLGYTDKMNDILASIDVLVSKPGGLTTTEALLKDVPMIVPYYIPG
                     QEEENLDFLSNCGAALRTTKKYNLSILLKVLIDDPSRLEMLKRNIKSIRKSNSAQNIA
                     NLILNIISG"
     gene            complement(1483..1704)
                     /locus_tag="PC414_003637"
     CDS             complement(1483..1704)
                     /locus_tag="PC414_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465987.1"
                     /translation="MKPIMNLEEHNAMKEKRVKKMKSKDKPKYDDFEDISFSNKKKTK
                     KNVRNKNAEFRNNYKNYQYDYEEDFYEPI"
     gene            complement(1845..2444)
                     /gene="recR"
                     /locus_tag="PC414_003638"
     CDS             complement(1845..2444)
                     /gene="recR"
                     /locus_tag="PC414_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HDF2465988.1"
                     /translation="MQVYTGPITRLIEEFSKLPGVGRKTAQRLAFHIINMNTNDVEAL
                     SKAIIDAKREIRYCSICCNITDTDPCSMCSNKSRDSSVICVVEDPRDVAAMERTREFK
                     GQYHVLNGVISPMDGIGPDMLKIKELIQRLGNQEVKEIIMATNPTIEGEATAMYIARL
                     VKPMGIKVTRIAHGLPVGGDLEYADEVTISKALEGRREI"
     gene            complement(2457..2807)
                     /locus_tag="PC414_003639"
     CDS             complement(2457..2807)
                     /locus_tag="PC414_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HDF2465989.1"
                     /translation="MAKKGFGGGMMPGGGNMNNLLKQAQKMQENMQKAQQELESKEVE
                     ASVGGGAVTVKVNGKKEVIDITIKPEVVDPDDIEMLQDLVLSAVNQALRNIDDIQASQ
                     MSKVTGGMNIPGLF"
     gene            complement(2866..4503)
                     /gene="dnaX"
                     /locus_tag="PC414_003640"
     CDS             complement(2866..4503)
                     /gene="dnaX"
                     /locus_tag="PC414_003640"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HDF2465990.1"
                     /translation="MHKALYRAYRPQKFEDVIGQDHIIKTLKNQIYSDNIGHAYLFCG
                     TRGTGKTSTAKIFSRAVNCLNKINEEPCNECEICESVLKDNTMDVVEIDAASNNSVDD
                     IRELRESVKYSPANAKYKVYIIDEVHMLSQGAFNALLKTLEEPPSYVIFILATTEPHK
                     IPATILSRCQRYDFKRVTVKDMTLRMKKICEDEGIDIDDKALNLIARNSQGALRDALS
                     ILDQCMSFGESKIDYKDVVELMGSVNIEQLFELSQCIVEQDTKKSLEILNEFVLWGKD
                     IRNLINDLIDHFRNLMVCKVSSELDEIISLPEETIEQLKIQSKNIDINDLIRILNILS
                     ITQDDIKSSSNPRVLVEITIMKIAQPMFDESKEALIKRVENLEKMIELGNFKSEKIGN
                     NKEKEYEVDREIDVKQENVVYEDVKNEDVILIESSWKNILKQIKKDKKMPIYALLSEV
                     KSFNVYSNMLYVIFDDKFDFAKTRLSSQDTINYLEKTIRDVLNRSFNVKIVLTSEVKD
                     INLEVKEKKDIGEEILKNIVSEEILEIKDSIDENESK"
     gene            complement(4580..4844)
                     /gene="ffs"
                     /locus_tag="PC414_003641"
     ncRNA           complement(4580..4844)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PC414_003641"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(4882..4973)
                     /locus_tag="PC414_003642"
     tRNA            complement(4882..4973)
                     /locus_tag="PC414_003642"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4937..4939),aa:Ser,seq:gga)
     gene            complement(4974..5429)
                     /locus_tag="PC414_003643"
     CDS             complement(4974..5429)
                     /locus_tag="PC414_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HDF2465991.1"
                     /translation="MESSFYMKEALKEAYKAYNKKETPIGAIIVKDNQIIARAHNLTE
                     TLKDSTAHAEILAIKQASEKLGGWRLTDCDLYVTMEPCIMCSGAIVNSRIKKLIIGTR
                     HVKNSYIEKQHEFKLDYFNNNNVKVAFDVLQEECSIILQEFFKALRKRD"
BASE COUNT         1754 a          979 c          633 g         2143 t
ORIGIN      
        1 tctatattta aattttttat ttacaagaat atttttaata taacatattt gataaaatta
       61 atttacattt ctttcacttg taatttttgc aatcttgtat tagattaata ttaaaaataa
      121 tttatcaata accttattca caaagacagt ttttatatat caaaatataa tataaatact
      181 atgctacatt ttaggaggat tttattatga gcaaaaaagt attaataatg tctgcttcca
      241 ctggtggtgg gcataatagg gctgctctag ctattaaaga agaacttact tccaaaacac
      301 ttgatggaga gccaattgaa tgtgaaataa tagatagttt gaaattagtt aataatacaa
      361 tggataaaat aatttcaaga ggatacgaaa aatctgctat atatactccc aaagcctatg
      421 gaagtgtata tagattatct gaaacaaatc ttttatcaaa aaatgaattt aaagataatc
      481 tactaataac attcatggct aaaaaattta aaaaacttat tagaagcgaa aaaccagact
      541 tgattatagg aacgcatcct tttccaatga ttgctctaag tacattgaaa aaaaacttta
      601 atttacataa taatgaatca aatgcttaca cagagcattt ttataaacat tatacaaata
      661 ccattaatgt tcctccgctt atttctgtat taaccgatta tactactcat tctacatgga
      721 tacaaaatga aattgattac tatattgttg gtcatgaata tgtaaaagaa ttattggtgt
      781 ttgatggagt tgaaccgagt aaaattagaa catttggaat ccctgttgaa aaatcttttc
      841 tctctcacag agataaaggt attgttcttt ctgagttgaa tttatctcct gataaattaa
      901 ctgttttact tatggggggt agctttgggg ctggaaatat aaaagagaca cttgatgaat
      961 tactggatac tgatagagac tttcaaatac ttgtaattac tggtaagaat gaaagcctaa
     1021 aagaaaaaat cgaaaaaaaa cttatgtctc gttatcatga taaaaatgta tgtgttcttg
     1081 gatacactga taagatgaat gatattttag cttctataga tgtattagta agcaaacctg
     1141 gtggacttac tacaactgag gctcttttaa aagatgtacc tatgatagtt ccttattata
     1201 taccaggtca ggaagaagag aatcttgatt tcttatctaa ttgtggtgca gcacttagaa
     1261 ctacaaaaaa atataacttg tcaatattac taaaagtttt aatagatgac cctagcagat
     1321 tagaaatgct aaaaagaaat attaaatcaa taagaaagtc aaattcagcc caaaatatag
     1381 ctaatttaat tttaaatata atttctggtt gataaaataa ataaaatccc tatagtatat
     1441 atattatcta ttagggattt tttattttct aattatttaa tattaaattg gttcataaaa
     1501 gtcttcttca taatcatact gataattttt ataattattt ctaaattcag catttttatt
     1561 tcgtacattt tttttagttt ttttcttatt agaaaaactt atatcctcaa aatcatcata
     1621 tttaggttta tcttttgatt tcattttttt aactcttttt tctttcattg cattatgttc
     1681 ttctaagttc ataattggtt tcatgttgca cccctatcaa aatttttaaa tgatttatac
     1741 aacaaattat acaatacaga agaaatttat tcaataaatc taaatcaaaa ttaatttgaa
     1801 tttaactcaa aaaattacaa aaatcgaggt attaacctcg atttttatat ttctcttctg
     1861 ccttctagtg cttttgatat tgtgacctca tctgcatact ctaaatcgcc tccaacagga
     1921 agaccatgag ctatccgagt gactttgatt cccattggtt ttactaacct agcaatatac
     1981 atggctgtag cttcaccttc aatagtagga tttgttgcca ttattatttc tttgacttcc
     2041 tgattgccaa gtctttggat aagttccttt atttttaaca tatctggtcc aattccatcc
     2101 attggagaaa ttactccatt taatacatgg tactgtcctt taaactctct cgttctctcc
     2161 atagctgcta catctcttgg atcttccaca acacatataa cacttgaatc tctactttta
     2221 tttgaacaca tgctacatgg gtctgtatct gttatattac aacaaattga gcaatatctt
     2281 atttctcttt ttgcatctat aatcgcttta gataaagctt ccacatcatt agtattcata
     2341 ttaataatat gaaaagccaa tctttgagca gtctttcttc ctactccagg aagctttgaa
     2401 aattcctcta taagtctcgt tatgggacct gtataaactt gcataaatac cacctactag
     2461 aataacccag gtatattcat acctccagtt accttactca tttgacttgc ttgtatatca
     2521 tcaatatttc tcaatgcttg atttactgca cttaaaacta aatcttgtag catttctata
     2581 tcatctggat caactacttc tggcttgata gttatatcta taacttcttt tttaccatta
     2641 acttttacag taactgctcc tccaccaact gaagcttcta cttctttact ttctaactct
     2701 tgttgagctt tttgcatatt ttcttgcatt ttttgagcct gctttaacaa gttgttcata
     2761 tttccaccac caggcatcat tccgcctcca aaaccttttt tagccataaa cactattcct
     2821 cctaaactta ttttcatttt attattatat cataatagta ttttattatt tactctcatt
     2881 ttcatcaata ctatctttta tttctaatat ctcttcactc actatgttct ttaatatttc
     2941 ctcaccaatg tcctttttct ctttaacttc aagatttata tctttaactt ctgatgtcag
     3001 tactattttt acattaaaac ttctatttaa aacatctctt attgtttttt ctaaataatt
     3061 tattgtatct tgagagctca gtctagtttt tgcaaaatca aatttatcat caaatataac
     3121 atacaacata ttagaatata cattaaaact ttttacttca cttaagagtg cgtatattgg
     3181 cattttttta tcttttttaa tttgtttaag tatattcttc caagatgact ctataagtat
     3241 aacgtcctca ttttttacat cttcataaac tacattttct tgttttacat ctatttctct
     3301 atcgacctca tactcttttt ctttattatt cccaattttt tcagacttaa agttccccag
     3361 ttcaatcatt ttttctaagt tctcaactct ttttataaga gcttctttac tttcatcaaa
     3421 cattggttga gcaattttca ttatggttat ttccactaaa acccttggat ttgaagatga
     3481 ctttatatca tcctgagtta ttgataatat atttaaaatc cttattaaat catttatatc
     3541 tatattttta gactgaattt taagttgttc tatagtttct tctggtaatg aaattatttc
     3601 atccaattct gatgaaacct tacataccat taaatttcta aaatgatcaa ttaagtcatt
     3661 tataagattt cttatatctt tgccccataa gacaaattca tttaatatct caagagactt
     3721 tttagtgtcc tgctctacta tacattgaga caattcaaat aattgttcta tatttacgct
     3781 tcccattaat tcaacaacat ctttataatc tatcttactt tctccaaaag acatacattg
     3841 gtctaatata ctaagtgcat ctctaagagc tccttgagaa tttctagcta ttaaattaag
     3901 agctttatca tctatatcaa ttccttcatc ttcacaaatc tttttcattc taagggtcat
     3961 atctttaact gtaactcttt taaaatcgta tctttggcat cttgataaaa tggttgcagg
     4021 tattttgtga ggctctgtag ttgctaatat aaaaattaca tatgatggag gttcctctaa
     4081 ggtctttaat aatgcattaa aagctccttg agacaacata tgaacttcat ctattatata
     4141 tactttatat ttagcattgg ctggagaata tttcacactt tctctaagct ctcttatatc
     4201 atctacacta ttatttgaag ctgcatctat ttctacaaca tccattgtat tatcttttaa
     4261 tacagattcg catatctcac actcattaca cggttcttca tttattttat tcaaacagtt
     4321 tactgctctt gaaaaaatct ttgcagtaga tgttttacca gtacctcttg ttccacaaaa
     4381 caaataagca tgtcctatat tatcactata tatttgattc tttaaagtct taataatatg
     4441 atcttgacca ataacatctt caaatttttg tggtctatat gctctatata atgctttatg
     4501 catcttttta cctctcttta gcaaaattaa taaaaaaatc cctcattatc taagggatta
     4561 gtcttttata tattaaatgc tgtacaccat ctatcgactt aattccatag gcgttaccaa
     4621 aacagttagc tcaacttagg cacccccacg gcacacaaaa gtgatcactt accgctgctt
     4681 ccttccggat ctgacggggt tcatgagctt tcattgcgca ggacctaagc atcaacacca
     4741 cttatttagg gcggccctac agaaaataag cctttagatg gaattcaatc ctgctatagc
     4801 ggattgcagg ttacagggca ccgctacctc cccatctagt acagcaaaat tacaaccata
     4861 tttaattact ttcattgttt ctggcggaga gagtgggatt tgaacccacg atacagtttt
     4921 acccatatac tcgctttcca ggcgagcgcc ttcgaccact cgaccatctc tccttaatct
     4981 ctttttctca atgccttaaa aaattcttgc aatatgatcg agcattcttc ttgcaaaaca
     5041 tcaaaagcta cttttacatt attattatta aaataatcta atttaaactc atgctgtttt
     5101 tctatatatg aattttttac atgtcttgtc cctattataa gcttttttat tctagaatta
     5161 actattgcgc cactacacat tatgcaaggt tccattgtaa catataaatc acaatcagtc
     5221 aacctccaac cacctaattt ttctgatgct tgttttatcg ctaaaatttc tgcatgtgcc
     5281 gtagagtctt ttaacgtttc tgtaaggtta tgagctctag ctattatttg attatcttta
     5341 actataatag cacctattgg tgtttctttt ttattatatg ctttatatgc ttcttttaat
     5401 gcttctttca tataaaatga actttccata attcctccca aaacttataa caaacttatc
     5461 ttatcataat tcattttata attcaactta attttatttt gtttattta
//
LOCUS       DAMJVK010000168         5494 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.173, whole genome shotgun sequence.
ACCESSION   DAMJVK010000168 DAMJVK010000000
VERSION     DAMJVK010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5494
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.173"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(154..1332)
                     /locus_tag="PC414_003644"
     CDS             complement(154..1332)
                     /locus_tag="PC414_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465992.1"
                     /translation="MQELKPVARISKSKLKDILDVFKTSIRKVEKIDYSACNSCAIKN
                     NPSFDSHKLNEFCSNCPKLKTISEFSYVTEKETELGTIEIVTNESKCMALSKSAIKQY
                     LAYHFIVSNKNYVRKAVSFQNIADICNVSIVTARHNHNVLLRVGLVYSTEIKRGKFDI
                     VIDEEYKNHLKKEDGGKGYLTMSLDTLNHLLSFENVNELKLEIKKLIWADAKGSKLGK
                     RIRFKKDNLISILPEYIKKSNKRTEAFLRSSKTLFDVSNDTIDTKRYETKETISEKYT
                     EPIRIQISKFFDNTGCSLSSNYSKVLSQINLDRYNLFEEDLRSKLDMEKDIIINDLVG
                     LSVQYGVTRVLAALDYMFNKYTVDEEDINYNEIRNPGAFIRTLIVQSINKNGSLAIAI
                     "
     gene            complement(1408..1674)
                     /locus_tag="PC414_003645"
     CDS             complement(1408..1674)
                     /locus_tag="PC414_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain containing protein"
                     /protein_id="HDF2465993.1"
                     /translation="MSLKYKDKEFVLVEKKNINELDNSNIKYIDLKDKQYYVVTQGKK
                     PKRFNLEDIKNIKKDLDNGISLRKCATKWNCSTRTIQDIKNNIY"
     gene            complement(2148..3716)
                     /locus_tag="PC414_003646"
     CDS             complement(2148..3716)
                     /locus_tag="PC414_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465994.1"
                     /translation="MSKDVKENISNEQKDLNKINLIYHIIYDNDRFKMITRKYFEIFR
                     LKGMSAEEISLKFEEFLYSLISDKFKKINTVNDVKKLRNQENSSSLKLKKRWSDMTLD
                     SLSEDTKSSRKKSSKYLKKELEIVESEYLKNGRIISAGVKNPKELKEQRKDFLDEWKS
                     SSSYNICDYPYLQELSTSKINSAFIHDILMCITEVLKDDYDFDINKIAIKTPPHISPG
                     IFLPFTKGKRYSHLNVKKNENEYMSDDYSAKNLSGKQMNFFYKFSIGQDENIENLKLE
                     LINPDTEKRALPKIYLDKQDLEIIRFVYTYSHMNSFSFYLGDLVKYLGLSDGKKNYIN
                     IKNRLLKLPYYTFYSNQTNDKGELKYEISFNLFSSTTIVNDENNNNRELVKITKSFIE
                     EVERVNTDIMYRNELEKLQLTQSINLAYFLEGRRSFLISCGEDIKNKSFRYDIEDLKF
                     DVHLNKSKTVKQNMEYLELGFNEIKENQFIIKDYKRGNSYFDVYFYEDFEKRKRLINN
                     TILSLPDYMIENKS"
     gene            complement(3721..4647)
                     /locus_tag="PC414_003647"
     CDS             complement(3721..4647)
                     /locus_tag="PC414_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465995.1"
                     /translation="MNYNLKNENVLSELLQNEDEKTRIGILKVYKVLIFWFAKEGKLV
                     EEENDYIVEIVGNDKEEIVRDMSQNKTYNAIMAIIFKINKIFKTLNYDIHFSKPKNLV
                     AEFDLSKYLTQDEVLQVCSKLTNAQDKFIIYAAFCGLIGKDAEDLRGIKIKDIDLEKR
                     LIKLKDTEFKLDKFLFDFTKDTLEQKEYKQYLEQDSNAAEFYLFNSNSEYLIKTKPDA
                     RYGNGLKEMGYSTIRKRLLIINDYLDNKVQLNFTNLKISGIMHQMHLNEKKYTQSGYW
                     TNKSLKEFKEDNNLNFKIQEVLSIYKQKYIKE"
     gene            complement(4780..5352)
                     /locus_tag="PC414_003648"
     CDS             complement(4780..5352)
                     /locus_tag="PC414_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HDF2465996.1"
                     /translation="MTWNDLKNNDITYIVEYINNKLNNSKSLTKVAIGLGVSESSIRK
                     YLTKRGYKRINDEYVFIGDNSMTKVVNNRQQSNNDNTFNMTIEDDNNHNIVIDNQFKN
                     NIVSLAKDYDKIQDVLNWFENKEDTNVIEVVQDGIKIDLHSKDAIRTTVRLNKDAWNL
                     FDEFCGKFREFNKSDLMSMALLEYIKKYDK"
BASE COUNT         1780 a          820 c          556 g         2338 t
ORIGIN      
        1 tctattgtgt atataatttc attaaaatag gcataaaaaa atagagctaa tagagatttc
       61 acgctcttta tagctctatt taagtatttt ataatttgaa tacaaacact aataggagta
      121 gccaaaatca ttttttttga actactccat atattaaatt gctattgcta aacttccatt
      181 tttatttatg ctctgaacaa tcaatgttct tataaatgca ccaggattac gtatttcatt
      241 ataatttata tcttcttcat caactgtata tttattaaac atataatcta atgctgctaa
      301 aactctagta actccatact gcaccgacaa tccaactaag tcatttataa taatatcttt
      361 ttccatgtct aatttacttc ttaaatcttc ttcaaataaa ttatatctat ctaaatttat
      421 ctgagataac actttactat aattagaact caaagagcaa ccagtattat caaagaattt
      481 cgatatttgt attctaattg gttctgtata tttctctgag atagtttctt tcgtctcgta
      541 acgctttgta tctattgtgt cattacttac atcaaataaa gttttagaac ttcttaaaaa
      601 ggcttctgta cgcttgtttg atttctttat gtattctgga agtatagaaa taagattgtc
      661 ttttttaaat cgtattcttt tacctaactt acttccttta gcatcagccc aaattaattt
      721 tttaatttca agttttaact catttacatt ttcaaaactt aataagtgat ttaaagtatc
      781 taatgacata gttaagtaac cttttccacc atcttctttt ttaagatggt ttttgtactc
      841 ttcatctatg actatatcaa atttacctct ttttatttct gtagaataaa ctaatccaac
      901 tcgtagtaat acattatggt tatgtctggc tgtaacaatg gaaacattgc atatatcggc
      961 tatattttgg aaggatactg cttttctaac ataattttta ttagatacta taaaatgata
     1021 tgctaaatac tgttttatag cacttttact taaagccata catttacttt cattagttac
     1081 aatttctatt gtacctagtt ctgtttcctt ttctgtaaca taagaaaact ctgatatagt
     1141 ttttaattta ggacaatttg aacaaaattc atttaattta tgactatcaa aagaaggatt
     1201 attttttatt gcacaagaat tacaagcaga ataatctatt ttttctactt ttcttataga
     1261 agttttaaaa acatctaata tatcttttag ttttgacttt gatattctag caactggttt
     1321 taattcttgc attttataat cctcctttct ctatatatga ttattataat cttaatgagg
     1381 tataaagtaa ataaatacct ctgtatatta ataaatatta ttttttatat cctgtatagt
     1441 cctagtgcta cagttccatt ttgttgcaca ctttcttaaa cttatgccat tatctaaatc
     1501 tttttttata tttttaatat cttctaggtt aaatctttta ggttttttgc cttgtgtaac
     1561 cacataatac tgcttgtcct ttaggtcaat atattttata ttagagttat ctaattcatt
     1621 tatattcttt ttttctacaa gtacaaattc tttatcttta tatttaagac tcataatttt
     1681 ctcctttgaa aaatcaataa tttcagaagt acggagctga tatagccatt ttggtgatga
     1741 ttccttttac tcaaatttat cttacccaaa aaataaattt atgtctatat ttgttaagta
     1801 cggagctgat acggtcattt agctcaaata gagtggtttc aacgtcttct gttaagtacg
     1861 gagctgatac ggtcatttag ctcagacata gtgatttcag aagacgtgag atttgaaatt
     1921 actaagaaaa gtaaaaaata tgttacaaaa aaaatttttc gacaaggaaa taattaaaaa
     1981 aaggtctatt ttttgttaag tacggaagat atacggtcat ttagtttttt tgaaaaatct
     2041 attttttgtt aagtacggag aatatacggt cattttactt tttttattaa gtttatttaa
     2101 aataaattcg acattttttg ttaagtacgg agaatatatg gccattttta agatttattt
     2161 tctatcatgt agtcaggtaa agaaagtata gtattattta taagacgttt tcttttttca
     2221 aaatcttcat agaaataaac atcaaaataa gaatttcctc ttttatagtc tttaattata
     2281 aattgatttt cttttatctc attaaaacct agttctaaat actccatatt ttgctttact
     2341 gttttacttt tattaagatg aacatcaaat tttaaatctt ctatgtcata tctaaaactt
     2401 ttatttttta tatcctcacc acaagatata agaaaagacc ttctaccctc taagaaataa
     2461 gctagattaa ttgactgtgt taattgtaat ttttcaagtt catttctata cattatatct
     2521 gtattaactc tttcgacttc ttcaataaaa gattttgtga ttttaaccaa ctctctatta
     2581 ttattgtttt catcatttac tattgttgta ctagaaaaca aattaaaact aatctcatac
     2641 tttaattcgc ctttatcatt agtttgattc gaataaaaag tataataagg taattttaag
     2701 agtctatttt tgatattaat atagttcttt tttccatctg aaagtcctaa gtactttact
     2761 aaatcgccta aataaaatga aaaagagttc atgtggctat aagtataaac aaatctaata
     2821 atttcaaggt cttgtttatc taaatatatt tttggcaaag cccttttttc tgtatcagga
     2881 tttattagct ctagtttaag attttcaata ttttcatctt gcccaattga aaacttatag
     2941 aaaaaattca tttgtttacc tgaaagattc ttagcactat agtcgtctga catgtattca
     3001 ttttcatttt ttttaacatt caagtgcgag tatcttttac cttttgtgaa aggtaaaaaa
     3061 attccaggtg aaatatgtgg tggagttttg attgcaattt tattaatatc aaaatcataa
     3121 tcatctttta gtacttctgt tatacacatt agtatatcat gtataaatgc ggaatttatt
     3181 ttgctagtac ttaactcttg taaatatggg taatcacata tgttataact agagcttgac
     3241 ttccactcat ctaaaaaatc ttttctttgc tcctttaatt cttttggatt ttttactcca
     3301 gcactaatga ttcttccatt ttttaaatat tcactttcaa caatttctaa ttcttttttt
     3361 aggtatttac tgcttttttt tcttgaagat ttagtatctt cacttagaga atctaaagtc
     3421 atatcagacc atctcttctt aagttttaat gatgatgagt tctcttgatt tcttaatttt
     3481 ttaacatcat ttactgtatt tatcttttta aacttatctg aaataagaga atatagaaat
     3541 tcttcaaatt ttaaacttat ttcttcagca gacataccct ttaatctaaa aatttcaaaa
     3601 tattttcttg ttatcatttt aaatctatca ttgtcatata taatatgata gattaaattt
     3661 attttattta aatctttttg ttcattactt atgttttctt taacatcttt actcataagg
     3721 ttactcctta atatattttt gcttataaat agatagaact tcttgtattt tgaagtttaa
     3781 attgttatcc tccttaaact ctttcaagct tttgttagtc caatatccag attgagtata
     3841 ttttttttca tttagatgca tttgatgcat aataccagaa atttttaaat tggtgaaatt
     3901 caactgtact ttattatcta aataatcatt aataataagt aatcttttgc gaattgttga
     3961 gtatcccatc tcttttaatc catttccata tctagcatct ggtttagttt taattaaata
     4021 ttcagaatta gaattaaata gataaaattc agcagcattg ctgtcttgct ccaaatattg
     4081 tttatactct ttttgttcta atgtatcttt tgtaaaatca aataagaact tgtccagctt
     4141 aaattctgta tcctttaact ttattaatct tttttctaaa tctatgtctt ttatttttat
     4201 accccttagg tcttcagcat ctttaccaat aagaccacaa aaagctgcat aaattataaa
     4261 cttatcttgt gcattagtta gcttactaca tacctgaagt acttcatctt gtgttagata
     4321 ttttgataaa tcaaattcag cgactaaatt ttttggttta gaaaagtgaa tatcgtaatt
     4381 taaagtttta aatatcttat taattttaaa aattatagcc attatggcat tgtatgtttt
     4441 attctggctc atatctctca caatttcttc tttatcatta cctactatct caacaatata
     4501 gtcattttct tcctcaacaa gctttccttc tttagcaaac caaaatatta acactttata
     4561 aactttgagt ataccaatcc ttgttttttc atcctcattt tgtaatagct ctgataatac
     4621 attttcattt tttaaattat aattcataaa ttcaaacatc ctttttttaa ttatatatat
     4681 attataacat ttaccttctc cgttttaaat agaaaataaa aaaaataacc tcaaaataat
     4741 gaggttattt aaattaaaac tacgatttta ttgtcatttt tatttatcat acttctttat
     4801 gtattctaat aaagccatac tcattaaatc agacttatta aactctctga attttccaca
     4861 aaactcatca aataaattcc atgcatcttt attcaaccta actgtagtcc ttatagcatc
     4921 ttttgaatgt aaatcaatct taataccatc ctgtacaact tctattacat ttgtatcttc
     4981 tttattctca aaccaattta atacatcttg tattttatca tagtcttttg ctaaagagac
     5041 tatattattt ttaaattggt tgtcaattac tatgttgtga ttgttgtcat cttctattgt
     5101 catattaaat gtattatcat tattactctg ttgtctattg tttactactt ttgtcatact
     5161 attgtcacca ataaacacat actcatcatt tatacgttta taacctcttt tagtaaggta
     5221 ttttcttatg ctactttcag acacaccaag ccctatagca actttagtca aactcttact
     5281 attatttaac ttattattaa tatattcaac tatatatgtt atatcattgt tttttaaatc
     5341 attccaagtc atatactcac ctctaaatta agtatactac atgagattat tgttgtcata
     5401 ccatattaac cagttgacta atagttttta tttacatata tttctaaagt gcaacaaact
     5461 ttgatataat ttatttataa ataacagatt aatt
//
LOCUS       DAMJVK010000169         5412 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.174, whole genome shotgun sequence.
ACCESSION   DAMJVK010000169 DAMJVK010000000
VERSION     DAMJVK010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5412
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.174"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            297..1823
                     /locus_tag="PC414_003649"
     CDS             297..1823
                     /locus_tag="PC414_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDF2465997.1"
                     /translation="MNESYWFLNSSPKEYNKLGENIKTDCLIVGGGITGLTTAYLLAK
                     EGKKVVLVEADKIGYGTSGRNTGKVTCQHDIFYSKIEKKYGLDKAKSYYNANNEALNL
                     VEQIIEENNIKCDFKRETSFVFTEKEDTIKDIKDEYRTCKKIGINCEYHETIENIPLD
                     IKGAISFTNQGQFNPKKYIDGLAKAAVNLGLKIYENTPVVDLEKGKICRVKTREDNII
                     EAENVIISSHSPWYDGLNFYFAKEYAERAYLMVAVLENKLADGMFISIDDPSITFRQY
                     NDGSENLLIFGGGDHKVGQGGTEKEIFDDLEHYGKEVFKVKDFKGKWSAQDNMSFDNV
                     PYIGYINKREDNIYVATGFSKWGITNGTAAGIIIKDLIINNNSDYKDTFNPSRLGSYF
                     SKDFIKENANVAINYVSGKLKIGSGDMPKNNGEGKIVNIDGKRYGVYKDDNGDFYIVD
                     TTCTHLGCELNFNSEEKTWDCPCHGSRFDYKGNILEGPALKPLKLYGHGDNDVNPKLL
                     "
     gene            1851..2408
                     /locus_tag="PC414_003650"
     CDS             1851..2408
                     /locus_tag="PC414_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465998.1"
                     /translation="MNKLLKPITILLYIVYILSAVFFVANKEYGSLGLVAISLVGTFI
                     LSFINKQTERLLSKELYIMLALFIMFSSLLGTCFNFYDINYYDDFLHLWSGIISCIVA
                     FSIIKYFYTQREISKMSKVFLVIFLFMFSMGVASLWEIGEFLMDTFVGTTTQAGGLED
                     TMIDMVDALIGTIITIPFIVKKSNI"
     gene            2930..3967
                     /locus_tag="PC414_003651"
     CDS             2930..3967
                     /locus_tag="PC414_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2465999.1"
                     /translation="MLKKWFGIVKKRQKSESVKEEGEVILKNEKILSEEKLIDEEGVV
                     VNIDNQILTKVVNDDNEIEEKIIEEDLLISENTIKLDDKEAIINDKNIELCKKEVQVE
                     SEKIGLNKFEGLDQNENYNLEKNVIEEKKVSECLTEEDLEYIKEIKIKRGKSIKAINL
                     YTKEEWVFDTHIQCSKKLKVPLGYIRENLKYGYMDYFGDAINYLSEVLNIDEYCKSEW
                     SYLDNSKSPSEIFNILNNKIFSIRLSNEKRNEILTNDKIEALKMNYRFECIDEEYDEY
                     FKKYKSIIKRGGKKKVELVNKKGDILEIFKSLEECAIYLQKEKNEVIQMLKYGDTKVG
                     RNFIRYSLRSI"
     gene            4078..5220
                     /locus_tag="PC414_003652"
     CDS             4078..5220
                     /locus_tag="PC414_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF2466000.1"
                     /translation="MSEEKNPKENTLDESKNSDNVNSKKDDVSDEKNTDNKVKKREIK
                     LNNKTLKAIVAIVAVGAICLGGGYVFGKEAGRKLPATSKHYNSSEVVATIGDKKITGE
                     MLRKRMEPLFYLNAKTTMTDDEIRSYESNMIETMITSDILSSEAAKEKISITNDQLDA
                     QYNNLMSSIESAMGMTKEEFLKQFDLTEKYIKDDMKKELVASKYLNEKSKVTDKEAEN
                     YYNKNKSNYLQVRASHILIKTVDDKGKQVSSSKKAELKKEAEEILKKAQAGEDFATLA
                     KKYSEDSSAESGGDLGFFGKGQMVESFEKAAFALKKGEVSNKLVESDYGYHIIKKTDE
                     KYQPFEEVKSDLVSSLTSEKQNLLIQNLKEKYNVKITDKYQMKAKE"
BASE COUNT         2291 a          446 c          952 g         1723 t
ORIGIN      
        1 agtaaactga ttgcataaaa atgattttta tataaaattt aagcagaaga ataaatttct
       61 aacgaaattg gatatttctt ctgtaaatac atattcaaaa aaagaattat aagagtttag
      121 tcagcagtcg tgtaagtaat aaggacttta taaatatata gaaatataat gatattaata
      181 taaccaagcg atttttaatt tagcttttta ataagcttat tttttatact atttataata
      241 aataaactcc atgatgggaa aactattact atttaatatg taaaggagta ttttaaatga
      301 atgaatcata ttggttttta aactcaagtc caaaagaata taacaaatta ggtgaaaata
      361 taaaaacaga ttgtcttata gtaggtggtg gaataacggg attgacaact gcatatttat
      421 tagcaaaaga aggtaaaaaa gtagtgcttg ttgaggctga taaaatagga tatggaactt
      481 ctggaaggaa tactggtaag gtaacttgtc agcatgatat attttactct aagatagaga
      541 aaaaatatgg attagataaa gcaaaatcat attataatgc aaataatgaa gctttaaatt
      601 tagttgaaca aataatagaa gaaaacaata taaaatgtga ttttaaaagg gaaacttctt
      661 ttgtatttac agaaaaagag gatactataa aggatattaa agatgaatat cgtacatgta
      721 aaaaaatagg aataaattgt gagtatcatg aaactataga aaatattcca ttagatataa
      781 agggggctat tagttttact aatcaaggtc aatttaaccc aaagaagtat attgatggat
      841 tggcaaaagc agcagtaaat ttagggctaa aaatatatga aaatacacca gtagtagacc
      901 ttgaaaaagg aaaaatatgt agggttaaaa caagagagga taatataata gaagctgaga
      961 atgttataat ttcatctcat agtccatggt atgatggatt gaatttttat tttgcaaaag
     1021 aatatgcaga gcgtgcttat ttaatggttg cagttttgga aaataaatta gcagatggta
     1081 tgtttataag tatagatgac ccatcaataa cttttagaca atataatgat ggaagtgaaa
     1141 atttactgat ttttggagga ggagaccata aagttggtca gggaggaact gagaaagaaa
     1201 tatttgatga tttagagcat tatggtaaag aagtctttaa ggtaaaagat tttaagggga
     1261 aatggtctgc tcaagataat atgagttttg acaatgttcc ttatattggt tatatcaaca
     1321 aaagagaaga caatatttat gtagctacag ggttctctaa atggggaatt actaatggaa
     1381 ctgctgctgg aataataata aaagatttaa taataaataa taattctgat tataaagata
     1441 cttttaatcc atccagacta ggtagctact tttcaaaaga ttttataaag gaaaatgcaa
     1501 acgtagcaat aaattatgta agtggaaaat taaaaatagg tagtggagat atgcctaaaa
     1561 ataatggaga aggaaagatt gttaatattg atggaaaaag atatggtgta tataaggatg
     1621 ataatggaga tttttatatt gtagacacta catgtactca tttaggatgt gaacttaatt
     1681 ttaacagtga agaaaaaaca tgggactgtc catgtcatgg ttctagattt gactataagg
     1741 gaaatatact tgaaggacca gctcttaagc cattaaaact ttatggacat ggtgataatg
     1801 atgttaatcc taagttatta taaaattatt tttaggaagg taaagtgtat atgaataaac
     1861 ttttaaagcc aataactata ctattgtata tagtttatat tttatctgcc gtattttttg
     1921 tagcaaacaa agaatatgga agtctaggtc tagtagcaat tagtttagtt ggtactttca
     1981 ttttgagttt tataaataag caaactgaaa gattattgag taaagaatta tatataatgc
     2041 ttgctttatt tataatgttt tcatcattgt taggtacttg ttttaatttt tatgatataa
     2101 attattatga tgattttttg catttatggt caggtataat atcttgtatt gtggcttttt
     2161 ctataattaa atacttttat acacaaagag agataagtaa aatgagtaaa gtgtttttag
     2221 ttatattttt atttatgttt agtatgggag tagcgagttt atgggaaata ggtgaatttt
     2281 taatggatac ttttgttggg actactacac aggccggagg gttagaagat actatgattg
     2341 atatggtaga tgctttaata ggcacaatta taactattcc ttttatagta aaaaaatcta
     2401 atatatagtt ttaaattttt tatattaatt ataatggatt taattaatca tgttgagttt
     2461 caaggtctat atgattaatt aaatccattt ttgttattta taattattat tgaatatatt
     2521 tttacatata agatataagg tgatagatag aaattgttgg attatgtaaa taattaatat
     2581 ataataccat tatgggcaat aaatacaaat caaatgataa tatagtctat ttatgtaaat
     2641 gttatattgt atcctaagta taatagtaag ttttttgata gtggtgtaga aagtaaaaat
     2701 ctactagcct aaattgatgt atggattagt ttaggaacag taattgcata tcttaagttt
     2761 gatgtcatgt tgggctacta gaaataggtc atctttttca cttgaatatc ttttctttaa
     2821 acatggggag tatcatatca aaaacatatg aaaattaagt gatttatgag aacagtaata
     2881 ttttatatga tagtatagaa tagtaataaa gtattaggag gaacattgag tgttaaaaaa
     2941 gtggtttggt attgtgaaaa aaagacaaaa aagtgagtca gtaaaagaag aaggtgaagt
     3001 aatattaaaa aacgaaaaaa tattatctga agaaaagttg atagatgaag aaggagttgt
     3061 agttaatatt gataatcaaa tattaactaa agtagtaaat gatgacaatg aaatagaaga
     3121 aaaaataata gaagaagatt tgttaattag tgaaaatacc ataaaattag atgataaaga
     3181 agcaattatt aatgataaaa acatagaatt atgtaaaaaa gaagttcaag ttgaaagtga
     3241 aaaaataggt ttaaataagt ttgaaggact ggaccaaaat gaaaattata atctagaaaa
     3301 aaatgttatt gaagaaaaaa aagtaagtga atgtttgaca gaagaagatt tagagtacat
     3361 aaaagaaatt aaaataaaaa gaggaaaaag tataaaagct ataaacttgt atactaaaga
     3421 agagtgggtt tttgacactc atatacagtg tagtaaaaaa ctcaaagttc cattagggta
     3481 cataagagaa aatttaaaat atggatatat ggattacttt ggagatgcaa taaattattt
     3541 aagtgaagta ttaaatatag atgaatactg caaaagtgaa tggagctatc tcgataatag
     3601 caaatctcca tctgagatat ttaatattct aaacaataaa atatttagta taaggctttc
     3661 taatgaaaaa agaaatgaaa ttttgacaaa tgataaaatt gaagcattaa aaatgaatta
     3721 tagatttgaa tgtattgatg aagaatatga tgaatatttt aaaaaatata agtctataat
     3781 caaaagaggt ggaaagaaaa aagttgaatt agtaaataaa aaaggtgaca ttttagaaat
     3841 atttaagtct ttagaagaat gtgccattta tttgcaaaag gaaaagaatg aagttataca
     3901 aatgttaaaa tatggagata caaaagtagg aagaaacttt ataaggtata gtttgagaag
     3961 tatttaattt cattttaaat tcataagagt ttcatttctg attcacacat ttaatattat
     4021 tttaatgtaa aataataaag gtgaagtatt gcacaattaa aattaaggag ggtattaatg
     4081 tctgaagaaa aaaatccaaa agaaaataca ttagatgaat ctaaaaattc agataatgta
     4141 aattctaaaa aagatgatgt gtcagatgaa aaaaatactg ataacaaagt taaaaaaagg
     4201 gaaataaagc taaacaacaa aacacttaaa gctattgtag caattgttgc agtaggagct
     4261 atatgtttag gtggaggcta tgtttttggc aaagaagctg gaagaaaatt gcctgctact
     4321 tcaaaacact ataatagcag tgaggtagta gctactattg gggataaaaa aataacaggt
     4381 gaaatgttaa gaaagagaat ggagccatta ttttatttaa atgcaaaaac tactatgact
     4441 gatgacgaaa tacgttcata tgaatcaaat atgattgaaa ctatgattac atctgatata
     4501 ttatcctctg aagcagcaaa agagaaaata agtataacta atgaccaact agatgctcag
     4561 tataataatt taatgagtag tatagaatca gcaatgggaa tgactaaaga agagttttta
     4621 aaacagtttg atttgactga aaaatatata aaagatgata tgaaaaaaga gctagtagca
     4681 tcaaaatatt taaatgaaaa atcaaaagtt actgataaag aagcagagaa ctattacaat
     4741 aaaaacaaga gcaattattt acaagttaga gcttcacata ttttaataaa aactgttgat
     4801 gataaaggta agcaagttag tagtagcaaa aaagctgaac ttaagaaaga agctgaagaa
     4861 atacttaaaa aagcccaagc tggggaagat tttgctactc tagctaaaaa atactctgaa
     4921 gatagtagtg cagaatcagg tggagattta ggtttctttg gtaaaggtca gatggtggaa
     4981 tcttttgaaa aagcagcctt tgcattgaag aagggagagg tatctaacaa acttgtagaa
     5041 agtgattatg gatatcatat aattaagaaa actgatgaaa aatatcagcc atttgaagag
     5101 gtaaaatcag atttagtaag cagtttaaca tcagaaaaac aaaacctact tatacaaaat
     5161 ctaaaagaaa aatataatgt taaaattaca gataaatatc aaatgaaagc taaagaatag
     5221 ataaagtcaa tttattaaaa aaaagaaaaa ttaactgtat ggataaaagc tatttttact
     5281 ctcaatatag agagtataaa atagctttta ttattttgca ataaaatcta tttatatata
     5341 tgaagtgata aagaaataac atgcaaaaag gtattaaaaa gaaatatttt taatttgaat
     5401 tttttcaata aa
//
LOCUS       DAMJVK010000170         5412 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.175, whole genome shotgun sequence.
ACCESSION   DAMJVK010000170 DAMJVK010000000
VERSION     DAMJVK010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5412
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.175"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(264..980)
                     /gene="dapD"
                     /locus_tag="PC414_003653"
     CDS             complement(264..980)
                     /gene="dapD"
                     /locus_tag="PC414_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HDF2466001.1"
                     /translation="MVNLNDAYEIARYIKEVKKKTPVKVYVNTNSLHFQSTDKFKVFG
                     SNGSYIFVGDYEDIMEVLDKHGNDITDTHVEYDRRNSAIPLKNTLSEHARIEPGAIIR
                     DMVTIEKNAVVMMGAVINIGAVIGEGSMVDMNAVIGARGTLGKNVHLGAGAVVAGVLE
                     PPSATPVIVEDDVLIGANAVILEGVRIGKGAVVAAGAVVTTDVEAGAVVAGSPAKVIK
                     MKDEKTADKTKLMEDLRNLD"
     gene            complement(1204..1953)
                     /locus_tag="PC414_003654"
     CDS             complement(1204..1953)
                     /locus_tag="PC414_003654"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDF2466002.1"
                     /translation="MLKVIATGYDGKMGKILADTIREDNELELVCVAARGLDSYEGDL
                     KIYEDMSTIVEEADVVIDFSHHSNLDNILSYVLKTKTPLVIATTGYNDDEMNKIREAA
                     KVIPVFQSYNMSLGVNVLVKLVKDAAKLLEGFDIEIIEKHHNRKVDAPSGTAVMIANA
                     IKEVLPNVENNYGRYGRSAKRQPNEVGIHAIRGGNIVGEHDALFAGDNEVLTISHQAQ
                     SRGIFASGSIAAAKYLVKQKPGFYNMDSMLG"
     gene            complement(2020..2907)
                     /locus_tag="PC414_003655"
     CDS             complement(2020..2907)
                     /locus_tag="PC414_003655"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDF2466003.1"
                     /translation="MIFKGSAVALVTPFTKDNKVDFDKLGELVEYQIANGTDAIVSCG
                     TTGEANTMTDEEQLATIKYVVEKVNKRVPVIAGSGSNDTMHSVNLSQEAEKLGVDALL
                     IITPYYNKANKAGLKRHFETIANSVKLPIILYNVPGRTCVNISPSLIVELAKIDNIVA
                     VKEASGDLGQVAEIASLVPDDFAIYSGNDDTILPLLSLGGQGVISVLANVCPQETHDL
                     VAKFFEGDIEGSRKLQLGMDALIAALFIEVNPIPVKTAMNLLGFNVGDLRLPLAEMDP
                     ANLEVLKKELVNFGLKVNA"
     gene            complement(3024..4025)
                     /locus_tag="PC414_003656"
     CDS             complement(3024..4025)
                     /locus_tag="PC414_003656"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDF2466004.1"
                     /translation="MKKVNVAIVGATGMVGRTFLKVLEERNFPIENLFLFSSSKSAGS
                     KVMFCGKEYIVEELKETSFTDRGIQIALFSAGGSISEKYAPIAASNGILVVDNSSQWR
                     MNPDVPLVVPEVNPEAIKNHKGIIANPNCSTIQAMVPLKALDDKYKIKRVVYSTYQAV
                     SGSGVKGVEDLEKGMNGNPTGFYPHQISYNCLPHIDSFTENGYTKEEMKMVNETMKIL
                     DNYDLKITATTVRVPVKNGHSESINVEFNNPFELDDLVKTLEEAPGVVVVDNPAKNEY
                     PTAVEFDDRDEVFVGRIRRDFSVDNGVNLWVVADNIRKGAATNAIQIAEMALSYDLV"
     gene            complement(4450..5331)
                     /locus_tag="PC414_003657"
     CDS             complement(4450..5331)
                     /locus_tag="PC414_003657"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDF2466005.1"
                     /translation="MLFKGSAVALVTPFTEDNNVNFEKLGELIEYHIENGTDALVVCG
                     TTGEATTMSESEIFAVIKYTVEKVNKRIPVIAGTGSNNTMLSVHMSQEAEKLGVDGLL
                     IITPYYNKTNEKGLKLHFETIANSVKLPIILYNVPGRTKVNIKPSVVAELAKIDNIVA
                     VKEASGDLAQVAEIAKLVPKDFAIYSGNDDTILPLLSLGGSGVISVLANICPKETHDL
                     VTKFFEGDIEGSKKLQLDMDALIAALFIEVNPVPVKTAMNILGFNVGDLRLPLAEMEE
                     TNLNVLKQELTNFGFKF"
BASE COUNT         1788 a          974 c          658 g         1992 t
ORIGIN      
        1 tattaaatta taaaaagttt aatagtttat tacattgata tattgaaaaa ttgaaggaaa
       61 taaaaatact tgtcagcaag aaataaaaat gatataagaa tgaataagag aattgtaaaa
      121 aaataaaatg attataaata aaagataaaa atattgtaaa aaagagggtg cctctggtga
      181 attaaattca cacagagaca cctttttaat taatttttta aattagtata ttattgttta
      241 attaatagta tagtactatt taattaatct aaatttctaa ggtcttccat aagcttagtt
      301 ttgtcagccg ttttttcatc tttcattttt ataactttag caggagaacc agcaacaacg
      361 gcaccagctt ctacatcagt agttacaaca gcaccagctg caacaacagc acctttaccg
      421 attctaacac cttcaagaat aactgcatta gctcctatta aaacatcatc ttcaactata
      481 acaggagtag cagaaggtgg ctctaaaaca ccagcaacaa cagcaccagc acctaagtga
      541 acatttttac caagagttcc tctagctcca ataacagcat tcatatctac catagaacct
      601 tcaccaataa cagctcctat atttataaca gctcccatca ttacaacagc atttttttct
      661 atagtaacca tgtctcttat tattgcacct ggctcaattc ttgcatgttc acttaaagta
      721 ttttttagag gaatagcaga atttcttcta tcatattcta catgagtatc tgttatatca
      781 tttccatgtt tatctaatac ttccattata tcctcatagt cacctacaaa tatataagaa
      841 ccattacttc cgaatacctt aaatttatca gttgattgga agtgtaaaga gttagtattt
      901 acatatacct taacaggtgt tttcttttta acttctttta tataccttgc tatttcataa
      961 gcatcgttta aatttaccat ttgttaattc tccttgtatt tcattatatt tcttttatta
     1021 tatacttatt tgtcaatttt agtatatact tacttcttat tatttatatt ttattttagt
     1081 tagtttttat tgtatagaca tagtattaaa taaatttatt atatatgttt gatttgtatt
     1141 gcgtattatt atatctatat tgcatatgtt taatttatat tgtgtattat tatatttatg
     1201 ctattatccc aacatgctgt ccatattata aaatcctggt ttttgcttaa ctaagtattt
     1261 ggcagcagct atagaaccac ttgcaaagat accacgagat tgagcttgat ggcttattgt
     1321 aagaacctca ttgtctccag caaatagagc atcatgttcg ccaacaatat ttccacctct
     1381 tatagcatga ataccaactt catttggttg tctttttgca ctacgaccat atctaccata
     1441 attattttca acgtttggca aaacctcttt tatagcgtta gcaatcataa cagctgtgcc
     1501 acttggggca tctacttttc tgttgtgatg tttttctatt atttctatat caaatccttc
     1561 tagtaattta gctgcatctt taaccaattt tactaataca tttacaccaa gagacatatt
     1621 atatgattgg aatacaggaa taactttagc cgcttcacgt attttgttca tttcgtcatc
     1681 attatatcca gtagtagcaa ttactaaagg tgttttagtc tttaaaacgt aagataagat
     1741 attatctaaa ttagagtgat gagaaaaatc tataactaca tcagcttcct caactatagt
     1801 actcatatct tcataaattt taaggtcgcc ttcatagcta tccagacctc tagctgcaac
     1861 acaaaccaat tctaattcat tatcttctct tatagtatcg gctaaaatct tacccatttt
     1921 tccatcataa ccagttgcta taacttttaa cataatttcc tcctatgatt aagggctaac
     1981 ttttgaccaa acttactttt taccatgcat taatctttac tatgcattaa cttttagccc
     2041 aaagttaact aattctttct ttaatacttc taaatttgct gggtccattt ctgctagagg
     2101 taatcttaaa tctccaacat tgaatcccaa taaattcatt gcagtcttaa ctggtatagg
     2161 atttacttct ataaataaag ctgcgattag agcatccata ccaagttgta attttctaga
     2221 accttcaata tcgccttcaa agaatttagc taccaaatcg tgagtttctt gagggcaaac
     2281 attagcaagt actgatataa ctccttgacc tcctaaagat aataaaggaa gtatagtatc
     2341 atcattacca gagtagatag caaagtcatc tggaactaaa ctagctattt ctgcaacctg
     2401 tcctaggtct ccactagctt cttttacagc aactatgtta tcaattttag caagttcaac
     2461 tataaggcta ggacttatat ttacacaagt tcttccaggg acattgtaaa gtataattgg
     2521 aagtttaaca ctgttagcta ttgtttcaaa atgacgctta agtccagctt tattagcttt
     2581 attgtaataa ggtgttatta ttaatagagc atctactcct aatttttcag cttcttgact
     2641 taagttaact gagtgcatag tatcattaga accacttcca gctataacag gaactctttt
     2701 attaactttt tcaacaacat acttaatagt agcaagttgt tcttcatcag tcatagtatt
     2761 agcttcacca gtagtaccac aagatactat agcatcagta ccattagcta tttggtattc
     2821 aactaattca cctaatttgt caaaatcaac tttgttatcc tttgtaaatg gtgtaactag
     2881 agcaacagca gaacctttaa atatcatata aaatcaatcc cctctttttt atttttgtgt
     2941 tttatattac agttacttct aatgcttgtt tttgagttac tattaatatt ttacaaaatt
     3001 atatatttat acaagtttgt atattatact aaatcgtatg ataaagccat ttcagctatt
     3061 tgaattgcgt tagttgcagc accttttctt atattatcag caacaaccca aagatttaca
     3121 ccattatcaa cactaaaatc tcttcttatt cttccaacaa acacttcatc tctatcatca
     3181 aattctacag cagttggata ttcgttttta gcaggattat caactactac aacacctgga
     3241 gcttcttcaa gtgtttttac taaatcatct aactcaaatg gattgttaaa ctcaacattt
     3301 atagattcac tatgaccatt ttttactgga actcttacag tagttgcagt tatttttaaa
     3361 tcgtagttat caagtatttt catagtttca tttaccattt tcatttcttc tttagtgtat
     3421 ccattttcag tgaatgaatc tatatgagga agacagttgt aagagatttg atgagggtag
     3481 aatccagtag gattaccgtt cattcctttc tctaaatctt caacaccttt aacaccagaa
     3541 ccagatacag cttggtaagt agagtaaaca actctcttaa ttttgtattt atcatcaaga
     3601 gctttaaggg gaaccatagc ttgtatagta gagcagttag gattagctat tataccctta
     3661 tgatttttta tggcttctgg attaacctca ggaactacta aaggcacatc tggattcatt
     3721 ctccattgac tagagttatc tactactaag attccatttg aagcagctat tggagcatac
     3781 ttttcactaa tacttccacc agcagagaat aatgctattt gtatgcctct atcagtaaat
     3841 gaagtttcct ttaattcttc tactatatat tctttaccac aaaacattac tttagaacca
     3901 gctgatttag aagaagagaa taaaaataga ttttctattg gaaaatttct ttcttctaat
     3961 acttttaaaa atgttctacc taccattcca gtagcaccaa ctattgctac atttactttt
     4021 ttcatataaa ttcaccccaa ttttaaatta ttaaaacatt attttatttt agttatacaa
     4081 gtaagatatg ttaatatttt tatatatcca aaaactttaa tattatatta acaactttaa
     4141 ttatatacaa aaaacgtaca aaggtatact ttgtacgtct aatttatcac tcaaagtaaa
     4201 ccctgtagct catcacctac aaaaataagt gacagtatta cacatgttat gtgcaatccc
     4261 agaaaatatt attgcaagaa atattttctt cggctgtaaa tcctttttcc aatcattaac
     4321 acctgccagc tactattgga tactcttagt tacagctacc tctaccctag gttaatagga
     4381 tattaaaatt tgattattct aataataata catatttaag aaaaaatcaa cttatttata
     4441 taaatattgc taaaatttaa atccaaaatt tgtcaattct tgttttaaca cattaagatt
     4501 agtttcttcc atttcagcta gaggaagtct taaatcacca acattaaaac ctaaaatgtt
     4561 catggcagtt ttaacaggaa ctggatttac ttctataaat aaagcagcaa ttaaagcatc
     4621 catatcaagt tgtaattttt ttgagccttc aatatcacct tcaaagaatt tagttactaa
     4681 atcatgtgtt tctttaggac atatgttagc aagtacagag ataacaccac ttccacctaa
     4741 tgataatagt ggtagtatag tatcatcatt accggagtat atagcaaagt ctttaggtac
     4801 taatttagct atttctgcaa cttgagctaa gtctccactt gcttccttca cagcaactat
     4861 attatctatt ttagcaagtt cagccacaac agatggcttt atattcactt tagttcttcc
     4921 tggtacgttg tataaaataa ttggcaactt aacgctattt gctattgttt caaagtgaag
     4981 tttaagacct ttctcattag tcttattata gtaaggagtt attattaaaa gaccatccac
     5041 ccctaatttt tcagcttctt gactcatatg aacagacaac atagtattgt ttgaaccagt
     5101 acctgctata acaggtattc tcttattaac tttttcaact gtatatttta ttacagcaaa
     5161 aatttcagat tcactcatag tagtcgcctc acctgttgtt ccacaaacaa ctagtgcatc
     5221 tgtgccattc tcaatgtgat attcgattaa ttcacctaat ttttcaaagt tgacattatt
     5281 atcctctgta aatggggtaa ctaaagcaac agcagaacct ttaaataaca tataatcaaa
     5341 tctccttttt tatctaaatt taatttttta agtacaataa taattatagt acaaattaat
     5401 taattttaat ag
//
LOCUS       DAMJVK010000171         5391 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.176, whole genome shotgun sequence.
ACCESSION   DAMJVK010000171 DAMJVK010000000
VERSION     DAMJVK010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5391
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.176"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            171..1007
                     /locus_tag="PC414_003658"
     CDS             171..1007
                     /locus_tag="PC414_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2466006.1"
                     /translation="MNIKKIFNNNVVVSSLEDGTEIIVTGAGVGFKKKVGDLIDENLI
                     SKKYFVQDDQRDKYNQILNKTSIEYFKISEEIIEKANEVLNTQVNDSIILALTSHIEF
                     AVQREKQGIKLPNLILNETKQLYREEFEFGLWAIDEIEKRIGIKLPEDEAGYIAIHII
                     NGLENSQKDEGINILEFSKQVIQIIEEVHGFKLDVNSLNYTRLITHLKFFVQRILRKE
                     TYNDSDIEDMYKLVNKNYNKPKVCTEEIAALVLDRFEYKISKEEELYLMIHINKITNS
                     GC"
     gene            1360..2937
                     /locus_tag="PC414_003659"
     CDS             1360..2937
                     /locus_tag="PC414_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2466007.1"
                     /translation="MKKNKLGLWNFFQMLGKTFMFPIALLSVSGMMLGLGAGFTDPKM
                     IEMVPFLGNEYVNLVLNFMLTIGLFAFNNLGALFAMAIPLGLLKKEKEFGAFSGLVGF
                     IAMHIGTNFYLTRADLLVPAEQMSTNGQAMIMGIQTYNTSVLGGIVAGLIVYAIYDKV
                     SNLKIPESLGFYSGPRLVPIVSLIVMSIVGLLIPIVWPPFFNAFQSLGRWISSAGPLG
                     YFSYAVAERVTIPFGLNHLVTSVFRFTPIGGTAVIDGETYFGTLNMFMAYVENNQIIP
                     LDLAGKMEQGKLMIQYGLAGAALAIYRTAKPENRKALKGLLISGVLTVIIGGISEPIE
                     FLFLFISPALFAFHTFMNGLANMVLPYLGVLMGFTGDLIAFISFGVLRGTATGWPIAV
                     LVAVLYFAIYYFVFKWAIIKFNIKTPGREDKEIKVSEGNGSNFKNLSTYKGQQMISAL
                     GGQSNILSLDNCVTRLRLKLQDVSLINEDAIKEAGGIAVVKLDEHTIQVIIGTQVYSL
                     RKQMDKAMEGHLEACND"
     gene            2930..4114
                     /locus_tag="PC414_003660"
     CDS             2930..4114
                     /locus_tag="PC414_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HDF2466008.1"
                     /translation="MINFDKKVDRLGTYCTQWDYVEDRFGKKDLLPFTISDMDLEFPF
                     EITEVLQERLNHRVLGYSRWKHDDFRNSVTNWYIKRFNCSVDSNWIYYSPSVMYSISK
                     LIEMLSNEGDGILINTPAYNAFYDVIINNNRNIIKSHLVNRNGFYSIDFNDFEKKCKK
                     SKIFILCNPHNPTGRVWSELELVKMIGICKNNDVKIISDDIHMDIVYDNRMTPILNVA
                     KDYLDSVFICTSASKSFNIPALTGSYVIIPNEGIRNKFENITRYRDFVNSPAILAILA
                     TMVCYDDCEYWLDELLKYLKENLKYTIDYIKDNLEPLKLVMPEGCYFAWIDFSELKIS
                     GEKFQKLLMDIGEVAIMSGSVYGEEGEFYLRLNVACSREKLEDGLNRIKKTMDYIEKR
                     IL"
     gene            4132..4731
                     /locus_tag="PC414_003661"
     CDS             4132..4731
                     /locus_tag="PC414_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDF2466009.1"
                     /translation="MGYLKTMVDVMTTEMNAIRCLIDEAGIEYESIVSEIANCEGKVI
                     FMGVGKSAHIGKKLAATFASTGTPSFFVHATEAVHGDLGMIESKDITILISNSGNSME
                     VVNCIKYIKAIGSKTIAFTSNRNSVLAKECDYALIYPAKDEADHLNLAPTTSSTITLV
                     LGDSIACALSKSSNFGSSDFYKYHPGGSLGEKLKTANNG"
     gene            4755..5225
                     /locus_tag="PC414_003662"
     CDS             4755..5225
                     /locus_tag="PC414_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2466010.1"
                     /translation="MFKMFKKKSIKSPISGKVIELSKIQDGVFSQKIMGEGVAIDSTG
                     DIVYAPINGKIVVVAETKHAFVIELENGMELLIHVGLDTVNLKGEGFEALVSMGEQVK
                     EGTPMLKINRPLIESKGISLITPVTITNHSEYNMNTCNVGNSVEGGKDTVIEFK"
BASE COUNT         2021 a          564 c         1031 g         1775 t
ORIGIN      
        1 tgataaaata tgacaaatta attgatgaca ataaaaaata ttttgttatt gtagtattat
       61 agggtaaatt gcaaaatata tacgaaagta aaattttaaa tattgacatt aaattaaata
      121 tactatacta tgtaagtgtg atgagagaat ttttttagag gggatttata gtgaatataa
      181 aaaagatatt taataacaat gtagtagtaa gctccctaga agatggcaca gaaataattg
      241 tcactggtgc tggagtagga tttaagaaaa aagtaggcga tttgatagat gaaaatttga
      301 tttctaagaa atattttgtt caagacgatc aaagggacaa atacaatcaa atacttaata
      361 agacctctat tgaatacttt aaaatttcag aagaaattat agaaaaggct aatgaagttt
      421 taaatactca agtgaatgat tcaataatat tagccttaac tagtcacata gaatttgctg
      481 tccaaagaga aaaacaaggt ataaaattac caaacttaat attgaatgag acaaaacaac
      541 tttataggga agaatttgaa tttggattat gggcaattga tgaaattgaa aaaaggatag
      601 gaataaaatt gcctgaagat gaagcaggat atattgctat tcacataatc aatggtttgg
      661 aaaattctca aaaagatgaa ggtattaata tactagagtt ttcaaaacaa gttattcaga
      721 taattgaaga agtacatgga tttaaattgg atgtaaattc attaaattat actagactta
      781 taactcactt aaagtttttt gttcaaagga ttctccgaaa agaaacttac aatgatagtg
      841 atattgaaga tatgtataag ttagtgaata aaaattacaa taaacctaaa gtgtgtactg
      901 aagaaattgc agcattggta ttagacagat ttgaatataa gattagtaaa gaagaagaat
      961 tatatttaat gattcacata aataaaataa caaacagtgg ttgttaggat tgtaactgat
     1021 aaagcaggca aaacctaagt cattgagctt aactattaga ttaagttttt tgatttaggt
     1081 ttttttatta gatacaatca aaatacagac agtgactttt aggattgtaa ctggttaagc
     1141 aggcaaaacc taaatcattg aatttaacta ttaggttaag ttttttgatt taggtttttt
     1201 tattagatac aatcaaatac agacagtggt tgttaggatt gtaactggtt gagcaggcaa
     1261 aacctaaatc attaaactta attattaaat taagtttttt gatttaggtt tttctataaa
     1321 tttgattatg tttttagata aaaataggag gggaaaatta tgaaaaaaaa taagttaggt
     1381 ttatggaact tcttccaaat gttaggaaaa acgtttatgt tcccaatagc actacttagt
     1441 gtttctggga tgatgctagg acttggggca ggttttacag accctaagat gattgaaatg
     1501 gttccatttt tagggaatga atatgttaac ttagttttaa acttcatgtt aacaatagga
     1561 ttatttgcat ttaacaactt gggagcatta tttgcaatgg caattccact aggattatta
     1621 aaaaaagaaa aagagtttgg tgctttttca ggattagtag gatttatagc aatgcatata
     1681 ggtacaaatt tttatttaac aagagctgat ttgttagtac cagcagaaca gatgtcaact
     1741 aatggtcaag ctatgattat gggtatacaa acatataata caagtgtttt aggtggtatt
     1801 gtagcaggat taattgttta tgcaatatat gataaagtta gtaatttaaa gataccagaa
     1861 tcattaggat tctacagtgg accaagatta gttccaattg ttagtttaat agttatgagt
     1921 atagttggat tattaatacc tattgtttgg ccaccatttt tcaatgcatt ccaaagttta
     1981 ggaagatgga tttcttcagc aggaccatta ggatattttt catatgcagt ggcagaaaga
     2041 gttacaattc catttggact aaatcatctt gtaacatctg tattccgttt tacaccaata
     2101 ggtggaactg ctgttattga tggagaaacg tactttggta cattgaacat gtttatggca
     2161 tatgtagaaa ataatcagat tatacctctt gatttagctg gtaaaatgga acaaggtaaa
     2221 ttgatgattc aatatggatt agctggtgct gcattggcta tctaccgtac agcaaaacca
     2281 gaaaatagaa aggcacttaa aggactttta atatcaggag tgttgacagt tattataggt
     2341 ggtataagtg aaccaataga gtttctattc ttattcatat caccagcatt atttgctttc
     2401 catacattta tgaatggtct agcaaacatg gtacttccat acttaggcgt gttgatggga
     2461 ttcacaggag atttaattgc atttataagt tttggtgtgt taagaggaac agctacagga
     2521 tggccaatag cagtattagt agcagtttta tattttgcaa tatactattt tgtatttaag
     2581 tgggcaatta ttaagtttaa tataaagacc ccaggacgtg aggataaaga aataaaagta
     2641 tctgaaggaa atggttcaaa tttcaaaaat ttgtctacat ataaaggaca acaaatgata
     2701 tctgctttag gtggtcaatc taatatatta tcactagata attgtgtaac tagattacgt
     2761 ttaaaattac aagatgtatc attaataaat gaggatgcaa ttaaagaggc tggtggtata
     2821 gcagttgtta aattggatga gcatacaata caagtaatta taggaaccca agtgtattct
     2881 ttaagaaagc agatggataa agctatggaa ggtcatttgg aggcatgtaa tgattaattt
     2941 tgataaaaaa gtagaccgat tgggtacata ttgtacccag tgggactatg tagaagatag
     3001 atttggtaag aaggatttgc ttccttttac aatttcagat atggatttag agtttccatt
     3061 tgaaataaca gaggtgttgc aggaaaggtt aaatcataga gttttgggat acagtagatg
     3121 gaaacatgat gattttagaa attcagttac gaattggtat attaaaagat ttaattgtag
     3181 cgttgatagt aactggatat attatagtcc aagtgtaatg tattcaatta gcaaacttat
     3241 tgagatgtta agtaatgaag gagatggaat ccttataaat actcctgcat acaatgcttt
     3301 ttatgatgtg ataataaata acaatcgtaa tattataaag tcacatcttg taaataggaa
     3361 tggattctat tcaattgatt ttaatgattt tgagaaaaag tgtaaaaaat ctaagatatt
     3421 tattttatgt aatcctcaca atccaactgg tagagtatgg agtgagttag aacttgttaa
     3481 gatgatagga atttgtaaga ataatgatgt caagataata tcagatgata tacacatgga
     3541 tattgtatat gacaatagaa tgacacctat cttaaatgtt gcaaaggact atttagattc
     3601 tgtattcatt tgtacatcag cgtctaaatc tttcaatata ccagcattga ctgggtcata
     3661 tgtgattata ccaaatgaag gtattagaaa taaatttgaa aatatcacta gataccgtga
     3721 ttttgtaaat tcgccagcaa ttctagcaat attagccaca atggtctgtt atgatgactg
     3781 cgaatattgg ttagatgaac tattaaaata ccttaaagag aatcttaaat atacgattga
     3841 ttatataaaa gataatttag aaccattgaa attggtcatg ccagaaggct gttactttgc
     3901 ttggatagat ttttccgaac ttaaaataag tggtgagaaa ttccaaaaat tattgatgga
     3961 tattggagaa gttgctatta tgtcggggtc tgtatatggt gaagaaggtg agttttattt
     4021 aaggttaaat gtggcatgtt caagagaaaa acttgaagat ggtttaaata ggattaaaaa
     4081 aactatggat tatattgaaa aaagaatttt atagtaggaa gggagatagt tatgggatat
     4141 ttaaaaacaa tggttgatgt tatgactact gaaatgaatg ccataagatg tttaatagat
     4201 gaagcaggaa tagaatatga aagtatagtt agtgaaattg caaattgtga aggcaaagta
     4261 atttttatgg gtgtaggaaa atctgcacat ataggtaaga aattggcagc tacatttgct
     4321 agtactggta cacctagctt ttttgtacat gcaacagagg cagtgcatgg agatttagga
     4381 atgattgaaa gtaaggatat tacaatttta atatcaaata gtggaaattc tatggaagtg
     4441 gtaaactgta taaaatatat taaagcaata ggttctaaga caattgcatt tacttcaaac
     4501 agaaattcag tactggcaaa agagtgtgat tatgctttaa tatatcctgc aaaggatgaa
     4561 gcagaccatt taaatctagc accaacaaca tcatctacaa ttactttggt tttaggagat
     4621 tctattgcat gtgcattatc taagagtagt aattttggtt cttcagattt ttataagtac
     4681 catccaggag gaagtttagg tgagaagtta aaaacagcta acaatggata aaagcaaaaa
     4741 ggagagtagt ttttatgttc aaaatgttta aaaaaaaatc aataaaatca cccatatcag
     4801 gaaaagtaat agagttatct aaaatacaag atggtgtatt ttctcaaaaa attatgggag
     4861 agggtgtagc tatagattct acaggagaca ttgtatatgc tccaatcaat ggaaagatag
     4921 tagtagtagc agagactaaa catgcatttg taatagaatt agaaaatgga atggaacttc
     4981 ttattcatgt tggtttagat actgtaaatt taaaaggcga aggttttgag gcattggtga
     5041 gtatgggaga acaagtgaag gaaggaacac caatgttgaa aattaaccgt cctttgattg
     5101 aatcaaaggg tatatcactt attactccag ttacaataac taatcattca gagtacaata
     5161 tgaatacatg taatgtaggc aatagtgttg aaggtgggaa agatactgta attgagttta
     5221 aataaagaac taaaatacaa ggttaatatt tataaatacc tataaactta tgttttataa
     5281 gattgattgt atagatttgt tgtataaaaa agaactcttt aattataaat taaggagttc
     5341 ttttttattt tgtaaatatt gtttagattt atatagaaaa acacttagta a
//
LOCUS       DAMJVK010000172         5379 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.177, whole genome shotgun sequence.
ACCESSION   DAMJVK010000172 DAMJVK010000000
VERSION     DAMJVK010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5379
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.177"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(341..541)
                     /locus_tag="PC414_003663"
     CDS             complement(341..541)
                     /locus_tag="PC414_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDF2466011.1"
                     /translation="MNNGIVKWFNNEKGFGFISMESGDDVFAHFSAIQTSGFKSLEEG
                     QKVSFDIVKGARGPQAENITIL"
     gene            complement(766..942)
                     /locus_tag="PC414_003664"
     CDS             complement(766..942)
                     /locus_tag="PC414_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2466012.1"
                     /translation="MFRDKMDKCTHMLTAYISNSYDYCDFIDTQLDDFILEYGEKVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(996..1085)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(1265..1465)
                     /locus_tag="PC414_003665"
     CDS             complement(1265..1465)
                     /locus_tag="PC414_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2466013.1"
                     /translation="MPKFTCNLKKYRQLKELTQEQLAEKVGVRRETIMRLEAGKYNPS
                     LKLAIDISKIVDTPIDELFIFD"
     gene            complement(1467..1817)
                     /locus_tag="PC414_003666"
     CDS             complement(1467..1817)
                     /locus_tag="PC414_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3796 domain-containing protein"
                     /protein_id="HDF2466014.1"
                     /translation="MIQKKFFMGFCGFLGFLSLRYFSSGNVADLTYIGFFAFFSNFII
                     AKINGDKADERYVQDEKAAMAFTGQLAIIELFILWCITIVSRNIELMCVLLSITYAIT
                     LNVYAIKLYILEEK"
     gene            1979..2167
                     /locus_tag="PC414_003667"
     CDS             1979..2167
                     /locus_tag="PC414_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2466015.1"
                     /translation="MTKYDFNFKLKVAKFYLNREGRDILIVKQYGVYDHSQVERWVNI
                     LGESELKIKKFSILYGSS"
     gene            complement(2644..2814)
                     /locus_tag="PC414_003668"
     CDS             complement(2644..2814)
                     /locus_tag="PC414_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2466016.1"
                     /translation="MHVYQPKTSEYLLNTYAKSYLSSIVFLFDYITKNESRYAFFIID
                     FPFLSLDIFYKF"
     gene            complement(3203..3691)
                     /locus_tag="PC414_003669"
     CDS             complement(3203..3691)
                     /locus_tag="PC414_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDF2466017.1"
                     /translation="MKNKKGFTLVELLVVIAIIGILAIVALPALFKNIEKAKIAKLEA
                     DISAIKSASLSYYADESKYTEGNIIWWTKKDGKITVNSGIGDEDPLAHKIENLGMPYN
                     GSYTLVSSNGSEEYLELNIIIDGEISKSGLDKLEEDYGSSIKIPNDKNMIITFLSNKS
                     DN"
     gene            4495..4869
                     /locus_tag="PC414_003670"
     CDS             4495..4869
                     /locus_tag="PC414_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2466018.1"
                     /translation="MKEEILIEKLLRAELIVMEYLWKKDTLMPKKEIVKEIKQIYGWH
                     KSTIKILLKRLVAKRYLARYIINSQSHYKIIDSKEYYTFKKKVLKSSKSIKIMRSLTT
                     THKNVSKEKLDLLDEYYRNLKE"
     gene            4974..5348
                     /locus_tag="PC414_003671"
                     /pseudo
     CDS             4974..5348
                     /locus_tag="PC414_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
BASE COUNT         2177 a          641 c          615 g         1946 t
ORIGIN      
        1 gaaacatata gatatgtttt aattgctttt tattaacaaa ttactaaaaa tgttatatca
       61 gaattgaata caaaagaaag agtatgtatt tatgagatag ttgaaaattt tgatgtgcca
      121 caactaaaat tattatatta ctatatattt aattaaaaga gtacttgtag gtttaagtac
      181 atactttata aatatatttt tgttagattt attttataaa aagtatttag actaagtatt
      241 atttttaagt attttcttgt tgaagttatt ttttcctttt ttacaaaatt cgtaaataaa
      301 aaaaagactc ttaaaagagt ctttccgaaa aatctatata ttataatata gttatatttt
      361 ctgcttgagg acctctagcg cctttaacta tatcaaagct tactttttga ccttcttcta
      421 atgatttaaa tcctgaagtt tgtatagctg agaaatgagc aaatacgtca tctccacttt
      481 ccattgatat aaatccaaat cctttttcat tattaaacca ttttactatt ccattattca
      541 ttaaaaatac tcctattaac ctatatcaag atatacttga tttacttgtg tattaccaca
      601 aatattattg tatcatagat taacataaat gtctatttaa ttataatttt tagttaacat
      661 ttttatttat tcttaatatg aaaataaatt taaacatttt atattaccaa tatattaatg
      721 taaatgtaga taaaactaaa aagggttttc tagaaaaaaa tctgtctaat tatacttact
      781 taccaatacc atcacttgat gcaagcaaga ttccacaact ttttctccgt actctaatat
      841 aaaatcatct aactgtgtat ctataaaatc acaataatcg tatgaattac taatataagc
      901 agttaacata tgtgtacatt tatccatttt atctctaaac ataatgtatt tatcccccta
      961 aaaaactttc atgaaaaaca aaaagaaaat atctttttgg caactggctg acataactta
     1021 ttttataagc gttagtccct atagctttgc gtcactaaat ttctctagtt ttgccgattt
     1081 agttttattc tacagattaa taatacaata gaattagtta tcattcaaca tatctagttg
     1141 aataattaat aaatttatat atgaacttta aattatttga aaaaataaaa agcccctctg
     1201 tcaggagtga cttaaaaatt gaatttaagt ttgtatttaa aacacatttt taataatgca
     1261 aaaactaatc aaaaataaac aattcatcaa taggtgtatc tactattttt gatatatcta
     1321 ttgctaattt caaagaagga ttatactttc ctgcttctaa acgcataatg gtttctcttc
     1381 taactcctac tttttctgct aattgttctt gagttaattc ttttaattgt ctatattttt
     1441 ttaggttaca agtgaattta ggcatattat ttttcctcta aaatataaag ttttatagca
     1501 taaacattta aagtgattgc atatgtaatt gatagtaaaa cacacattaa ttctatattc
     1561 ctagacacta tagtaataca ccataaaatg aataattcta taatagcaag ctgtcctgta
     1621 aatgccatag ctgctttttc atcttgtaca tacctttcat ctgctttatc accatttatt
     1681 tttgctataa taaagtttga gaaaaaagca aaaaatccaa tatatgtaag gtcagcaaca
     1741 ttgccagatg aaaaatatct aagacttaaa aatccaagga aaccacaaaa acccataaaa
     1801 aattttttct gaatcatttt ttatcctcct tagtaatttt aaaagagata tatttatatc
     1861 tttatgatac aaatatatct cttttatatt cttttgtcaa tagaaaatca gtattataca
     1921 atatttatac tgcaccataa ttgttagagc aaaaatctaa tgcttgagat gtttatgtat
     1981 gactaaatat gattttaatt tcaaattaaa agtagctaaa ttttatttga atagagaagg
     2041 aagagatatt ttaatagtaa aacaatatgg agtttatgac cattctcaag ttgaaagatg
     2101 ggttaatata ttaggagaga gtgaactaaa aataaaaaaa ttttctatac tatatggttc
     2161 aagttagatg ttataaacta tatgatgata actaaatata gtgtacaata aatcgctaag
     2221 tattttggaa taaacaatag cacagttata gcaacatgaa aataaaaatt tttacatagt
     2281 ggtgttgatg ccttttctaa aatgaaagga agaccataat gaataaaaac aaaaacacta
     2341 aagaaaaaat aaattaacaa gagaacaaga attagaatat gaaaataaat ttttaaaaga
     2401 tgagcttgag tttttaaaaa agatgtgtgt ctcagggaag ccacattaaa aaacagtggt
     2461 atcattatta atcaaaagaa agttagatgt attatcaaaa aattagaact taaatgcata
     2521 gcattttttc ataaatcaca taaatataac tcctataaag gtataattgg aaaagtaaaa
     2581 aataataaaa caaatagaag atttaaaaca tcaatagcat atcagaaaat aacaaatgat
     2641 atgctagaat ttataaaata tatctaatga taaaaatgga aaatcaatta taaaaaaggc
     2701 atatctcgat tcattttttg ttatatagtc aaataagaat actatagatg aaagatacga
     2761 ttttgcatat gtattaagta agtattctga tgtttttggt tgatacacat gcaataaaga
     2821 agttgtgttt gcagataaat tagcaaaact atagtataag ttgcaacaat aaagaaaatg
     2881 aaaattatca tactaaagca taaggcatgt tacaatttta ttagatagga cattaacata
     2941 cactaaagta ctttttatta taaagagtac ttattttttt gaaattcatt aatgtataaa
     3001 ctatcaagaa tattactcaa cataatttaa aaacaacttg aataggtatt tttaaattat
     3061 gtgatataat aaaaaataga agtgtatgcc aatacacttc tgtagttata actaagcatt
     3121 ctattcttga ggagtgcttt tttcattttt ataaccaact ctttttactt taacaataat
     3181 tatcagacct atatatttat ttttaattgt ctgatttatt agataaaaaa gttattatca
     3241 tatttttatc atttggtatt tttattgaac taccataatc ttcttctaat ttatctagac
     3301 cacttttact tatttctcca tctataatta tgtttaattc taagtattct tcactaccat
     3361 tagatgacac taaagtgtac gaaccattat aaggcatgcc taaattttct attttatgtg
     3421 ccaaagggtc ttcatcacca ataccagagt ttactgttat ttttccatct tttttagtcc
     3481 accatattat gtttccttca gtatattttg attcatctgc atagtagcta agagacgcac
     3541 tttttattgc acttatatca gcttcaagtt tagctatctt tgctttttct atatttttaa
     3601 ataatgctgg aagtgctact attgccaata ttcctattat agcaattact actaataatt
     3661 ccactagagt aaatcctttt ttatttttca tttatttccc ctccatataa aattaataat
     3721 atataaattt ttattttgtt aatttctact ttctatattt taccatatat agacaaaaaa
     3781 atcactttct atgacaaata tactattatt ttacaattta tggaaatgat ttaaaatgac
     3841 atttcttgtt agaaaagata taagaaatgg attaaaataa agaaaactac agtcatatca
     3901 atgtcaataa tattatttaa aagcagatga aaataaaaag aggactcaga aaataatgtc
     3961 tatttaatta tacttactta ccaataccat aacttgatat aaacaagatt atactacatt
     4021 tttttatgct ctagtataaa atcatctaac tgtgtatcta taaaatcaaa ataattatat
     4081 gaactatcag tataataagt caaaatatgt atacatgaat acatatttta aaagatatat
     4141 attattacat acctaaaaaa ctttcataaa aacaaaaata aaatatgctt ttagtaactt
     4201 gttaacataa cttataattt attttataag cattagccta tatagctttg tgctactaaa
     4261 tctctttgat tctaacgatt tagtgctatt ctacaactaa aataatacaa tagagctatt
     4321 tattgtttgg tagcattgtt tgtaaattaa acaaatttat acatatgaat attattttac
     4381 attcaaaaaa acagactaaa tatgtagaat atttggtaaa acttacctct agtttttgtc
     4441 ttaaaaagct cctattatta acttaagtta gaagaattaa ggaagtgaca taaattgaaa
     4501 gaagaaatat taatagaaaa attattaaga gcagaattaa tagtaatgga gtatttatgg
     4561 aaaaaagaca ctctaatgcc taaaaaagaa attgtaaaag aaataaaaca gatttatgga
     4621 tggcataaaa gtacaataaa aatactacta aaaaggctag ttgctaaaag gtatttggct
     4681 agatatatta taaattctca atctcattat aaaataatag atagtaaaga atattatact
     4741 tttaagaaaa aggtactaaa atctagtaaa agcataaaaa taatgcgttc acttaccact
     4801 acacataaaa atgtaagtaa agagaagtta gacctattag atgagtatta tagaaactta
     4861 aaagaatgat atagtaaaaa tcgaatgtaa ttatttacct taattctttt gtttgtaaaa
     4921 ctttattgat acagaattta acatatacat aaattggtaa aggaatgtat actatgaaaa
     4981 ttagtaaatt accagaagcg gaattaaaag ttatgagata tatttgggaa tcagaaaagg
     5041 tattaatatc aagagaaatc accaagtcta tggaacaaaa atatgaatgg aaagatacaa
     5101 ctatatttac agtattaaaa agattactaa agaagagagt ttttagaagc ggagaaaata
     5161 gataagtgta cacactataa tgtattagta aaaaaaaata atatcagaga ttttaaacaa
     5221 aagaattttt aaagaataca cataaaaatt caatattaag tttaatgtca gcactatgta
     5281 gtaaggatga tgaagtagat ggtgatattt taaatttatt agaagaatat tttaagaatt
     5341 tgaaataaga aaatgacaaa ttgatacata ataaataaa
//
LOCUS       DAMJVK010000173         5103 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.178, whole genome shotgun sequence.
ACCESSION   DAMJVK010000173 DAMJVK010000000
VERSION     DAMJVK010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5103
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.178"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(507..2642)
                     /gene="rnr"
                     /locus_tag="PC414_003672"
     CDS             complement(507..2642)
                     /gene="rnr"
                     /locus_tag="PC414_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HDF2466019.1"
                     /translation="MIPGLKESLLGLINESAYNPLKKEELAEIFDIHSSEMPMFYNFL
                     NELEEDGYICFTKKGKIVSPNQMGYFVGKFVSHKKGFGFVESDVEYTQDLFISSDNIN
                     GAMHNDRVMAEIVVPATEEKRAEGRIVKVIKREITRIVGTFQSSKTFGFVTPDNKKFT
                     KDIYIPKKHFSGAVDNDKVVCEITVWPQEDRKPEGKIIEILGQKGERGVEIDSIIREH
                     GLPEEFPKKVLQEAEAVAVEIPQEEIKRRRDLRDLNIFTIDGDDAKDLDDAISIEVLP
                     NGNFKLGVHIADVTHYVREKNKLDKEALKRATSVYLVDKVIPMLPKTLSNGVCSLNPF
                     EDKLTLSIFMEIDHKGNVVKHEICESVINSKARMTYTEVSDILEKDDEKLKKTFEHVV
                     EDFKNAEILARILMSRREKRGAIDFNFPEAKIILNGKGEVVDIKPYERRISNKIIEEF
                     MLISNETIAEHFYWMGIPFVYRIHETPSLEKMEELSKFISTFGYIIKGDKEEVHPKAL
                     QGIIEKIKGKKEEGAISTIMLRSLKQAKYSPECSGHFGLAAKFYCHFTSPIRRYPDLQ
                     IHRIIKESLNNKISGKRQEQLTTIVDYASVQSSEKERKAELAERDVHDFYKALYMKDK
                     VGQEFEGVVSSVTSFGIFVELDNTIEGLIRLANMNDDYYIYDESSYSVLGERTKKSYK
                     IGDLVKIKVESVNVDFKEIDFEIVEKIEE"
     gene            3235..3849
                     /locus_tag="PC414_003673"
     CDS             3235..3849
                     /locus_tag="PC414_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HDF2466020.1"
                     /translation="MEEVIAKIITIENGFKEIENIAKKIVKNNDSKKCYYLSIYLYRS
                     EYYQVRELAVFILGFISVTISEALLFLKDNVSKDENWRVQEILAKSFDYYCSEVGYEK
                     ALPVIKEWLNSDSPNIKRAVTEGLRIWTSRDYFKSNPDVAISLLSSLKEDDSEYLRKS
                     VGNALRDISKKYPDLVKKELDSWDISNKKVEFVYKLASKCILAK"
     gene            3867..4229
                     /locus_tag="PC414_003674"
     CDS             3867..4229
                     /locus_tag="PC414_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2466021.1"
                     /translation="MKLRDEYTCPLELTHDMIKGKWKSIILWRLRIGPTSLSKLEKEI
                     NGINQKMLLEQLKELIEFGLVDKKTYEGYPLKVEYSLTPIRGKQILDALAIMQDIGIS
                     FMLDNGMEDILKEKGVLK"
     gene            4335..4832
                     /locus_tag="PC414_003675"
     CDS             4335..4832
                     /locus_tag="PC414_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HDF2466022.1"
                     /translation="MKVTSTGIVNGVIEDKYGKRGTQFNEGGMPTYSLPLKFEDAPKN
                     TVSFAVFLEDKDSVPVCGFAWIHWLAVNITKDELNENESVTATDFVQGTTSWHGKLGG
                     LDRLEASVYGGMAPPDRPHVYEIHVFALDTILDLEKGFYMNEFFKAIEGHVLDTFTLK
                     GSYSN"
BASE COUNT         1664 a          768 c          615 g         2056 t
ORIGIN      
        1 tacattaaat atttcttaat tacttatctt tcagctagct ttttctatta aatgtttctc
       61 aattacttat ttttcaatta gccacttata ttaagcattt cttaattact tatttttcaa
      121 ttagccactt atattaaaca tttcttaatt atttattttt cagttggctt tttctattaa
      181 atgtttctta attacttatc tttcagttag ctctttctat taaacatttc ttaattattt
      241 atttttcagt tggctttttc tattaaatgt ttcttaatta cttatctttc agttagctct
      301 ttctattaaa catttctcaa ttacttattt ttcaattagc tactttcatt aaatatttct
      361 taattattta tctttcagtt aactatttcc attagatatt tctcaattac ctatttttat
      421 catttatttt atagagtatt gtgcactata tatttataca tatcataaat actacaactt
      481 gttatctctt attttagctg ttttatctac tcctctattt tttcaactat ctcaaagtca
      541 atttctttaa aatctacatt aacactttca acctttatct taacaaggtc tcctatctta
      601 tatgacttct tagttctttc tcctaaaaca ctataagaac tctcatcata aatatagtaa
      661 tcatcattca tatttgcaag tcttatcaat ccttctatgg tattatccaa ctcaacaaat
      721 attccaaatg aagttacgct agatacaaca ccctcaaatt cttgaccaac tttatccttc
      781 atataaagag ccttatagaa atcatgtaca tctctttcag ctaactctgc ctttctttct
      841 ttctcggatg attgaacaga tgcataatcc actatagtag taagctgttc ttgtcttttt
      901 ccacttattt tattatttaa agattctttt attattctat gaatttgaag gtcaggatat
      961 cttcttattg gtgaagtaaa gtgacagtag aatttagctg ctaatccaaa atgtccacta
     1021 cattctggtg agtacttagc ttgttttaaa gaacgaagca ttatagtact gatagctcct
     1081 tcttcttttt tgccctttat tttctctatt ataccttgta atgcttttgg atgtacttcc
     1141 tctttatcac cttttattat atatccaaat gtagatataa acttactcaa ttcttccatt
     1201 ttttccaaag atggtgtctc atgtattcta tacacaaacg gtattcccat ccagtaaaaa
     1261 tgttcggcaa ttgtctcatt acttataagc ataaattctt ctattatctt attagatatt
     1321 cttctttcat atggctttat atctacaact tcgccttttc catttagaat tatctttgcc
     1381 tctgggaaat tgaagtctat agctcctctt ttttcccttc ttgacataag aatcctagct
     1441 aaaatttcag catttttaaa atcttcaaca acatgttcaa aagtcttttt aagtttttca
     1501 tcatcctttt ctaatatatc agatacttct gtataagtca ttctagcttt tgaatttatt
     1561 acagattcac atatttcatg tttaaccaca tttcctttat ggtctatttc cataaatata
     1621 gaaagagtta atttatcttc aaatggattt aagctacata ctccatttga aagagttttt
     1681 ggcaacattg gaattacttt atctactaaa taaactgatg tagctctttt taaggcttcc
     1741 ttgtcaagtt tatttttctc tcttacataa tgtgtaacat cagctatgtg aactcctagt
     1801 ttgaagtttc cattaggtaa tacttctatt gatatggcat catctaagtc ttttgcgtca
     1861 tctccatcta tagtaaatat gtttaaatct cttaaatctc ttcttctttt tatctcttct
     1921 tgaggaatct caacagctac agcttctgct tcttgcaata ctttctttgg aaattcttct
     1981 ggaagaccat gttctcttat tatagaatct atttctacgc ctctttcacc tttttgacct
     2041 agtatctcta taattttgcc ttctggtttt ctatcctctt gtggccatac tgtaatttca
     2101 catactacct tatcattatc tactgctcca ctaaaatgtt ttttaggtat atatatatct
     2161 ttagtaaatt tcttgttgtc tggtgttaca aatccaaaag ttttacttga ttggaaagta
     2221 cctactattc tagttatttc tctctttatt acttttacta ttcttccttc tgctctcttt
     2281 tcttcagttg caggtactac tatttctgcc ataactctat cattatgcat agcaccattt
     2341 atattgtcac ttgagataaa taaatcctgt gtatattcaa catcagattc tacaaatcca
     2401 aaaccctttt tgtgtgatac aaattttcca acaaagtatc ccatttgatt gggagatact
     2461 atttttcctt ttttagtaaa gcagatataa ccatcttctt ctaattcatt taaaaaatta
     2521 taaaacattg gcatttctga tgaatgtata tcaaatattt ctgctaattc ttcttttttt
     2581 agaggattat atgcactttc atttattaat cctagcaaac tttctttaag tcctggtatc
     2641 atttttccct cctgttttac ttttcattat attattattt atattgtctt ttatatttct
     2701 tcagacaaaa cattttttaa ttatcacata attttctata ttttcttatt attataagtt
     2761 aaaattactt atttatattt ttgatttaat gtaagctttg tcatactaac ttcaaacaaa
     2821 ttatgatgtt ttataaatta tttgtaaata tcattataat tacttaagtt attctttatc
     2881 ataattgctt aaattactct ttatcaccta attatacacc atcaaatagg attggtaaaa
     2941 tattcatctc atataagtat tactatataa taatttattc tattagtata ctcaaatcat
     3001 gtaaaattta tataactcaa ttaatttttt aaatacaact ttataatata aaacttataa
     3061 taaaagtaaa tttatatgtt aaatgaggtt gattttttat tattttttat ttatgctagt
     3121 actaaagact tgcaaacgtt ttttgacaag tctttatttt gtctaaatta aacaaacttt
     3181 tttacaaata aatacataaa atttaaacta atctatttat tggaggagat aaaattggaa
     3241 gaagtaattg caaaaattat tactatagaa aatggattca aagaaattga aaacattgct
     3301 aaaaaaattg taaaaaataa tgactcaaaa aagtgttatt atctatcaat ctacttatat
     3361 aggtcagaat attatcaagt aagagaattg gctgtgttta tacttggttt tatatcagta
     3421 actatttctg aagctttgtt atttttaaaa gataatgtga gtaaagatga aaactggaga
     3481 gtacaagaaa tactagctaa atcatttgat tattattgca gtgaagtagg atatgaaaag
     3541 gctcttcctg ttataaaaga atggcttaat tctgatagtc caaacataaa aagggctgtg
     3601 acagaaggtc ttcgaatttg gactagtaga gactatttta aatctaatcc tgacgttgct
     3661 atatcactac tttctagttt aaaagaagat gatagtgaat acttgagaaa gtctgtaggt
     3721 aatgctctta gagatataag taaaaaatat ccagatttag taaaaaaaga attagattca
     3781 tgggatatat caaataaaaa agttgagttt gtatataagc ttgcttcaaa atgtatcttg
     3841 gcaaagtaaa atggaggtga acttatttga aacttcgtga tgagtatact tgcccattag
     3901 aactaacaca tgatatgata aaaggcaaat ggaaatctat tatattatgg agattacgca
     3961 taggaccaac ttccttatca aaacttgaaa aggaaatcaa tggtattaat caaaagatgt
     4021 tacttgaaca acttaaagaa ttaattgaat ttggtcttgt agataaaaag acatatgaag
     4081 gttacccact taaagttgaa tactcactta cacctattcg agggaaacaa atattggatg
     4141 ctttggctat aatgcaagat ataggaatta gctttatgtt ggataatggt atggaagata
     4201 ttcttaagga aaaaggtgtt ttaaaataaa tagataccca caaaaaagta agtaatttac
     4261 cattattttc aactggtata taattaaatt atagatatat tctattaatt tattataata
     4321 ggaggtaaat tattatgaaa gtaactagta caggtatagt aaatggtgtt atagaagata
     4381 aatacggtaa aagaggaact cagttcaatg aaggtggtat gcctacatat tcactacctc
     4441 taaagtttga agatgctcca aagaatacag tatcctttgc tgtattttta gaagataaag
     4501 actctgttcc tgtatgtggg tttgcatgga tacattggtt agctgtaaat ataacaaaag
     4561 atgagttaaa tgaaaatgaa agtgtaacag ctactgattt tgtacaaggg actactagtt
     4621 ggcatggcaa actaggaggt cttgacagat tagaggccag tgtatatggt ggaatggctc
     4681 ctccagacag acctcatgtc tatgaaatac atgtatttgc acttgatact atccttgacc
     4741 ttgaaaaagg attctatatg aatgaatttt tcaaagctat tgaagggcat gttttagata
     4801 catttacact aaaaggaagt tattctaatt aattaggttg actatagcta atcattaatt
     4861 aaatgctaca tttaattgat gattagctta attgttatta gaatattttc tgcactttat
     4921 cttaatatat attttagttg actggtattt taatttctat tagattatta tcaatttata
     4981 taaattgatt ttagcttaaa acttacttat ctatttcagt ttctattaga attattatta
     5041 gactcattaa aataaaagca ctaatataac taataatata tgttaaattc attaactcta
     5101 cat
//
LOCUS       DAMJVK010000174         5070 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.179, whole genome shotgun sequence.
ACCESSION   DAMJVK010000174 DAMJVK010000000
VERSION     DAMJVK010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5070
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.179"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            50..1933
                     /locus_tag="PC414_003676"
     CDS             50..1933
                     /locus_tag="PC414_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HDF2466023.1"
                     /translation="MSNKNKENITLSNQIKGTIQNSLTKEAILHIPMSNYAYGYDRET
                     LHIRLRTKKNEAKRVILRIGDQYVWDKGGAGGGNLNASGVGWSGGTNIVMSKEVETEL
                     FDYWIAKCKPLNKRSRYGFIIEGQEEKLLFTEKRIIELGNENDEKELCEIGNFFGYPY
                     LNYIDIPKVPNWVKDTVWYQIFPDRFANGDPSINPEGVDKWGAIPTRDNFTGGDLQGV
                     IEHLDYLSDLGINGIYFCPITIGKTNHRYDTVDYMEVDPTLGDKETLKKLIEEAHKRN
                     IKIMLDAVFNHIGYYSKQWQDVVQNKENSRYKNWFYIKDMSKIDTPIEQIDEKNIPYE
                     TFGCEKYMPKLNTENSEVIKYLLDVGKYWIQEFDIDAWRLDVSNEVDHVFWRKFREEV
                     KKVKPDIYILGEIWHGSLPWLMGDQFDSVMNYLMSEAMKKFFCTDEINAEEFKYMIND
                     VMVSYPIQVSEVIFNLLGSHDTTRILTYANGNIDRFKLAYMFMFVQTGCPCIYYGDEI
                     GMEGELSSISEGQRKCMEWNEEKWNKDIFDFMKKIIRLRKENKELRSISNEWVLADKE
                     NGTIILKKESISIIINNSSKNIILTLPDYLANKKVKDLYEEEIVDLKEELMLEKYKFI
                     ILK"
     gene            2157..2837
                     /gene="pgmB"
                     /locus_tag="PC414_003677"
     CDS             2157..2837
                     /gene="pgmB"
                     /locus_tag="PC414_003677"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDF2466024.1"
                     /translation="MIEAFIFDLDGVITDTAYYHYMAWRKLAHKVGIDIDTKFNESLK
                     GISRMESLDRILEFGNKKYSFSEEEKVRMAEEKNNYYVSLIDEITSNDILPGIESLLI
                     DVKSNNIKIGLSSASKNAINVLNHLGISDKFDFIADAGKCKNNKPHPEIFLMSAKGLN
                     VNPQNCIGIEDASAGIDAINSANMFSVGVGNYENLKKANLVVDSTNQLKFEYIQEKYN
                     EYIVRRII"
     gene            2834..>5070
                     /locus_tag="PC414_003678"
     CDS             2834..>5070
                     /locus_tag="PC414_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_097546124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HDF2466025.1"
                     /translation="MKCLFSNVDEWKIIQDKIEIKENRLAESIMSIGNGYMGMRGNYE
                     ENYSGDTHKGSYIAGVWFPDKTRVGWWKNGYPEYFGKIPNSVNYIGVRIFIDDVELDL
                     AKCKVEDFHRELDMKNGILNRRFIVNINDKKFEVRVTRFLSISVKELAVIKYSIKSLN
                     SNTKIKFVPYLDSNIKNEDSNYEEKFWNGISSSSLEYKGSILSRTKENAFNTPVFTVG
                     SMMYIKTNGDVEAVSHESDYNYCENSITMDVLKNEVASIEKYVSITSTRDYKESELLR
                     KCEDILNRELSKSYDDILNEHIKLWNKRWESADVKINGDKSSQQGIRYNLFQLFSTYY
                     GEDSRLNIGPKGFTGEKYGGATYWDTEAFCVPVYLGVADKEVTRNLLEYRYNQLEQAK
                     DNAKKLGLKGALYPMVTFDGVECHNEWEITFEEIHRNGSIVYAIYNYTNYTGDYKYIK
                     EKGIDVIIEIARFWASRVHLSTKKDLYMIHGVTGPNEYENNINNNWYTNYIAKWCLDY
                     AVENILRLEKECNECIDRNNVTREEVEFWKIISNKMYLLYDEELQIIIQNDNFLDKEF
                     VEVKDLPKENFPLNQNWSWDKILRSCFIKQADVLQGIYYFGNRFTKEEKKRNFDFYEK
                     YTVHESSLSSSIYSIIASEIDNLEKAYELYSRTARLDLDNYNNDTNDGLHITSMSGSW
                     LSIVHGFAGMRTWNETLSFDPKLPKEWKNYSFNINYRDNIINVDVCSDLIKIENLKGK
                     PIKIIV"
BASE COUNT         2093 a          459 c          958 g         1560 t
ORIGIN      
        1 aacctatctt tagattatat aaattattat aaaggaggga aattttaaaa tgtctaataa
       61 aaataaagaa aatataactt taagtaatca aatcaaagga acaattcaaa atagtttaac
      121 aaaagaagct atattacata taccaatgag caattatgcc tatggttatg atagagaaac
      181 actacatata agacttagaa ctaaaaaaaa tgaagcgaaa agagtaattt tacgaatagg
      241 agaccaatat gtgtgggata aaggaggtgc aggaggagga aatttaaatg catctggagt
      301 tggttggtct ggtggaacaa atatagtaat gagtaaagaa gtagaaactg aattatttga
      361 ctattggata gctaagtgta agcctttaaa taaacgctca cgatatggct ttataataga
      421 aggtcaagag gaaaaacttt tatttacaga aaaaagaata atagagttgg gaaatgaaaa
      481 tgatgaaaag gaattatgtg aaataggtaa tttttttgga tatccatatt taaattatat
      541 agatatacct aaagtgccaa attgggtgaa agatactgtt tggtatcaga tatttccaga
      601 tagatttgca aatggtgacc cgtctataaa cccagaagga gtagataaat ggggagctat
      661 tccaaccaga gataatttta caggtggaga tttacaaggt gtgatagaac acttagatta
      721 tttgagtgat ttgggaataa acggaattta cttttgccct attacaattg gaaaaaccaa
      781 tcatagatat gatacggtag attatatgga ggtagaccct acattaggag ataaagagac
      841 tttaaaaaaa ctaatagaag aagcgcataa gagaaatata aaaataatgt tggatgctgt
      901 ttttaatcat ataggatatt attctaaaca gtggcaagat gtagttcaaa ataaggaaaa
      961 ttctcgatat aaaaactggt tttatataaa agatatgagt aaaattgata ctccaataga
     1021 acaaatagat gaaaaaaata taccttatga aacctttggg tgtgaaaaat atatgcctaa
     1081 acttaataca gaaaattcag aagtaataaa atatttattg gatgtaggca agtactggat
     1141 acaggaattt gatatagatg cgtggagatt agatgtttca aatgaagtag accatgtttt
     1201 ttggagaaag tttagagaag aagtcaaaaa agtaaaacca gacatttata tattgggaga
     1261 aatttggcat ggtagtttac catggttaat gggtgaccaa tttgattcag ttatgaatta
     1321 cttgatgtca gaagccatga aaaaattctt ttgtacagat gagataaacg ccgaagaatt
     1381 taagtatatg ataaatgatg taatggtaag ttatccaata caagttagtg aagttatttt
     1441 taatttactt ggaagtcacg atactacaag aatattgact tatgctaatg gaaatattga
     1501 tagatttaaa cttgcatata tgtttatgtt tgtacaaaca ggatgtcctt gtatatacta
     1561 tggtgatgaa atcggaatgg aaggagaatt aagttcaata tctgaaggtc aaagaaaatg
     1621 tatggaatgg aatgaagaaa aatggaataa ggatatattt gattttatga agaagattat
     1681 tagacttcgt aaagaaaata aagaacttag gtctatatca aatgaatggg tgttagcaga
     1741 taaggaaaat ggaacgatta tattgaaaaa agaatctatt tctataataa taaataattc
     1801 atctaaaaat ataattttga cgttaccaga ttatttagca aataaaaaag taaaagattt
     1861 atatgaagaa gaaatagttg atttaaaaga agaactaatg ttagagaaat acaagttcat
     1921 tattttaaaa taatacaaaa aagtatttat tttagatttc atgttaaatt attatacagt
     1981 ttaaacaagt ctttataaac gataaaaaat atggtctaca agtgagtgaa ttgaaagtag
     2041 attttaaatt aaagactgag ttagagtaga taattttata aataatatct taaatacaag
     2101 atagaaaata aattgattat tagtctaaaa atttcttgta gaaagaggaa ataagaatga
     2161 ttgaagcatt tatatttgat ttagatggtg taattacaga tactgcatat tatcactata
     2221 tggcttggag aaaactagct cataaagttg ggatagatat agacacaaaa tttaatgaat
     2281 ctttaaaagg cataagtaga atggagtcat tggacaggat attagaattt ggaaataaga
     2341 aatattcatt ttcagaagaa gaaaaagtga gaatggctga agaaaaaaat aattactatg
     2401 tatcattgat agatgagata acttcaaatg atattttgcc aggcatagaa agcttgttaa
     2461 ttgatgtaaa atctaataat ataaagatag gtctatcttc tgcaagtaag aatgcaataa
     2521 atgtattaaa tcatttgggt ataagtgaca aatttgattt tatagctgat gcaggaaaat
     2581 gtaaaaacaa taaaccacat ccagagatat ttcttatgtc agcgaaaggg cttaatgtaa
     2641 atccacaaaa ttgcataggg atagaggatg caagtgcagg tatagacgct ataaactcag
     2701 ctaatatgtt ttctgttggt gttggaaatt atgaaaactt aaaaaaagca aatttagttg
     2761 tagattcaac aaatcaatta aagtttgaat atatacaaga aaagtataat gagtatatag
     2821 ttaggagaat tatatgaaat gtttatttag taatgtagat gagtggaaga ttatacaaga
     2881 taagattgaa atcaaagaaa atagattagc agagtccata atgagtatag gcaatggata
     2941 tatgggtatg agaggaaact atgaggaaaa ttatagtgga gatacacaca aggggtctta
     3001 tatagctgga gtatggtttc cagataaaac tcgtgttggt tggtggaaaa atgggtatcc
     3061 agaatacttt ggtaaaattc ctaattcagt caactatata ggtgtaagaa ttttcataga
     3121 tgatgtagaa ttagatttag caaaatgtaa agtagaagat tttcatagag aattagatat
     3181 gaaaaatggt attttaaatc gtaggtttat tgtaaatata aatgataaaa aatttgaagt
     3241 tagggttact aggtttttaa gtatatctgt taaggaactt gcagtaatta aatacagtat
     3301 aaaatcatta aattctaata ctaaaataaa atttgtacca tatttagatt caaatataaa
     3361 aaatgaagat tcaaactatg aggaaaagtt ttggaatgga atttcttcaa gttctttaga
     3421 atataaaggg agtattcttt ctcgaacaaa ggagaatgca tttaatacac cagtattcac
     3481 tgtgggttca atgatgtata ttaagacaaa tggtgatgta gaagctgtat ctcatgagag
     3541 tgattataat tactgtgaaa attcgataac aatggatgtt ttaaaaaatg aggttgcaag
     3601 tatagaaaaa tatgtatcta taacctcaac tagagattat aaggaaagtg agctattaag
     3661 aaaatgtgaa gacattttaa atagagaact atctaaaagt tatgatgata tattaaatga
     3721 acatataaag ttatggaata aaagatggga aagtgcagat gtaaaaataa atggagataa
     3781 aagtagccaa caaggtataa gatataattt attccaacta ttttcaacat attatggaga
     3841 agattcgcga ttaaatatag gtccaaaagg atttactggt gaaaagtatg gaggagctac
     3901 ttattgggat acagaagcgt tttgtgttcc agtatatcta ggtgtagcag ataaggaggt
     3961 aactagaaat cttttagaat ataggtacaa tcagttagaa caggcaaagg ataatgcaaa
     4021 aaaactaggt cttaaaggtg ctttatatcc aatggtaaca tttgatggag tagaatgtca
     4081 taatgaatgg gagataacat ttgaggaaat ccaccgaaat ggaagtatag tatatgcaat
     4141 atataactat acaaattata caggtgacta taaatatata aaagaaaaag gaatagatgt
     4201 aataatagaa atagctagat tttgggcaag tagagttcat ttgtctacaa aaaaagattt
     4261 gtatatgata catggagtaa caggtccaaa cgagtatgaa aacaatatca ataataattg
     4321 gtatactaat tatatagcta aatggtgttt agattatgct gtagaaaata tcttacgatt
     4381 agagaaagag tgtaatgaat gtattgacag aaataatgtg actagggaag aagttgagtt
     4441 ttggaaaata atatcaaata aaatgtatct gctgtatgat gaggaactac aaataataat
     4501 tcaaaatgat aattttttag ataaagaatt tgtagaagta aaggatttac caaaagaaaa
     4561 ttttcctctt aatcaaaatt ggtcttggga taagattctt aggtcgtgtt ttataaaaca
     4621 agcagatgta ttacagggaa tttactattt tggaaataga tttactaaag aagaaaaaaa
     4681 gagaaatttt gacttctatg aaaaatatac tgtacatgaa tcttctttat cttctagtat
     4741 ttattctatt attgcctcag aaatagataa cctagaaaag gcttatgaat tatattcaag
     4801 aacagctagg cttgatttag ataattacaa taatgataca aatgatggac tgcacataac
     4861 ttcaatgtct ggttcatggc tttccattgt acatggattt gcaggcatga gaacatggaa
     4921 tgaaactcta tcttttgacc ctaagttgcc aaaagagtgg aaaaattatt ctttcaatat
     4981 aaattataga gataatatta ttaatgtaga tgtatgtagt gatttaatta aaatagaaaa
     5041 tttaaaaggt aaaccaataa aaattattgt
//
LOCUS       DAMJVK010000175         4833 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.180, whole genome shotgun sequence.
ACCESSION   DAMJVK010000175 DAMJVK010000000
VERSION     DAMJVK010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4833
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.180"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(232..1275)
                     /locus_tag="PC414_003679"
     CDS             complement(232..1275)
                     /locus_tag="PC414_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HDF2466026.1"
                     /translation="MGTKIVLSIVLIVVVVAISLTCIRVIKQSKVGIIMRLGKFQKVA
                     ETGVHFLIPFLDKMAYVIDLREIVIDFPPQPVITKDNVTMQIDTVVYYKVTDPVRYVF
                     EIANPIAAIENLTATTLRNIIGELDLDETLTSRDIINVKMRTILDEATDKWGIKVNRV
                     ELKNIMPPQDIQVAMEKQMRAERERREAILQAEGNKSAAILQAEGEKQSAILTAEAKK
                     EAMVRVAEGEKESAILVAEGEAEAIRQTAIAKAQGEAEMIKRTQMATAEGLKLVFSAM
                     KEADIDNNILALKSMEALEKMAEGKSTKLVLPSEAVNFLGTFKGIKEVMSDDNKEVLD
                     IKEVLNDNESLKK"
     gene            complement(1296..1742)
                     /locus_tag="PC414_003680"
     CDS             complement(1296..1742)
                     /locus_tag="PC414_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HDF2466027.1"
                     /translation="MKLIWLIVAVLFGIAEMLTPSLTLIWFSVGAVILIFLSSFIESI
                     FLQILIFAVISIAMLVVATKKIVKKDKGYKSNTNLQAMMSKKGIVTEEISPNNTGLVV
                     VEHETWTAISIDGEKIEKGSTVEVLKIEGVKLVVKKVDATVNVTNQ"
     gene            complement(1916..3496)
                     /locus_tag="PC414_003681"
     CDS             complement(1916..3496)
                     /locus_tag="PC414_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HDF2466028.1"
                     /translation="MLIGTSKLSRNVDTACVEEVGIPMIVLMENAVISAMKNMDIDMY
                     NSYTVVCGVGNNGGDGLGIARHLNTYGKEVNVFLVGNLEKLSECSKINYNVLLNMGIN
                     IININKNDLSEKNYGEENEVDKIIKVEKQNIIKNKNLTIFKDRVSKSDVVVDAIFGTG
                     LKRDITGIFKEVIDIVNDNSKNTYSIDIPSGIDSDNGNVLGTCIRANKTISFEFYKRG
                     FLNYDTSALIGDVVVEKIGVPDFITRKYHDEEFITDKTFVKNNIRRRDKHGFKSDFGK
                     VSIVAGSKGFYGASFIATESAVKSGSGLVTLISSEDVQEKVSIRLTEAMTLNFEEERL
                     NKLLNSSDAIGFGPGMGDNSQTFDKLLKIVENSNCPIVLDADGLNVMKDRCYKFLEWK
                     NRFVITPHLGEMARLTGDSIGYIREHRVDVAKEFAQKYNLVVLLKGYQTVITDGKKTY
                     INPTGNSCMATGGMGDCLLGMITSFIGQGMDILEATVSGAYIHGYIGDKLSKAMYTVN
                     ATDLISNISLTMKELLDI"
     gene            4295..>4833
                     /locus_tag="PC414_003682"
     CDS             4295..>4833
                     /locus_tag="PC414_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2466029.1"
                     /translation="MSGIISVMTQSLILSIMALGVYITYKILDFPDMSADGSYTMGAS
                     IVAFSLTNGISPVVATLMAILCGCTAGLVTGILHIKFKISNLLSGILVLGMLYSINLR
                     IMGKSNIPLFSFKHLFNGEISPIVLALAFVFICKVLLDLFLKTGLGYTLKGVGDNSQM
                     IKSLGINIGSIKILGLMIS"
BASE COUNT         1547 a          835 c          493 g         1958 t
ORIGIN      
        1 gcttatttgc tatatgtttt atataatatt atataacact ttttacatta gtaaaggata
       61 aattgtataa aaattctgtt aatttatttt tataatttca tctttctaat aaaaacaaag
      121 aagaagactt aaaaatttat aagtcctctt cttttcaacg tgcatttttg tgctatattt
      181 aattaattcg acctttaatt tccatatcct tcctatgata acaaaactct tttatttttt
      241 taatgattca ttatcattta aaacttcttt tatatcaagt acttctttgt tatcatcact
      301 cataacttcc tttattcctt tgaatgttcc taggaaatta actgcttctg aaggtaaaac
      361 cagttttgtt gacttacctt cagccatttt ttcaagagct tccatagatt ttaatgctag
      421 aatattattg tctatatcag cctctttcat tgctgaaaaa actaatttta aaccttctgc
      481 tgttgccatt tgagttcttt ttatcatttc agcttcacct tgtgccttag caatagctgt
      541 ctgtcttata gcttcagctt caccttctgc cactaatata gcagattctt tttcaccttc
      601 tgctacacgt accatagctt cttttttggc ttctgctgtt aatatagcag attgtttttc
      661 accttcagct tgaagtattg cagctgactt attaccttct gcttgaagta ttgcctctct
      721 tctttctctt tctgctctca tttgtttttc cattgcaact tgaatatcct gaggaggcat
      781 tatatttttt aactctactc tatttacttt tataccccat ttatctgttg cttcatcaag
      841 aatagttctc atttttacat ttattatatc tcttgatgtt aatgtttcat ctaaatcaag
      901 ttcaccaatt atatttctta gtgttgtagc tgttaaattt tcaatagcag ctattggatt
      961 agctatctca aacacatatc taactgggtc tgtaacttta taatatacaa ctgtatcaat
     1021 ctgcatagtt acattatctt tagttataac tggttgaggt ggaaaatcta ttacaatttc
     1081 tctaaggtca attacatatg ccattttatc taaaaatggt attaagaaat gtacacctgt
     1141 ttcagccact ttttgaaact taccaagtct cattattata cctactttag attgttttat
     1201 aactcttata caagttaaac ttattgctac gactactact attaatacaa tacttaaaac
     1261 aattttagta cccatactat aaatcctcct ttattctatt ggtttgtcac attaactgtg
     1321 gcgtctactt ttttaacaac taattttacc ccttcaattt ttaatacttc tactgtagaa
     1381 cctttttcta tcttttcacc atctatagaa atagccgtcc atgtttcatg ttcaactaca
     1441 actagacctg tgttatttgg agaaatctct tctgtaacaa tacctttttt agacatcatt
     1501 gcttgtaaat tagtgttaga tttatagcct ttatcttttt ttactatttt tttagtagct
     1561 acgactaaca ttgctataga aattactgca aatattaaaa tttgtaagaa aatactctct
     1621 ataaagctac ttaaaaatat aagtattaca gctcctacac taaaccatat caatgttaag
     1681 cttggtgtta acatttctgc tattccaaat aagactgcta ctatcaacca tattagcttc
     1741 atactatcta ccctcttttc atatgaatta taaaacccta tataaattat atgtttatac
     1801 aatgtttttg aatataaatt taaattacaa aacaataaaa taaattaatt aattaatagt
     1861 attatatcaa catactttgt taaaattgtt acaaagtagt atcaaatatc accaattata
     1921 tatccaacaa ctctttcatt gttaatgaaa tgttacttat taaatccgta gcatttaccg
     1981 tatacattgc tttgctcaat ttatctccta tatagccatg tatatatgct ccactaactg
     2041 tagcttccaa tatatccata ccttgaccta taaatgaagt aatcattcca agcaaacaat
     2101 cacccattcc accagttgcc atacagctat ttcctgtagg atttatatat gtttttttac
     2161 catctgttat gacagtttga taacctttta aaagcaccac cagattatat ttttgagcaa
     2221 attcttttgc aacatctact ctatgttctc ttatataccc aattgaatct cctgtcaacc
     2281 tggccatttc tcccaaatga ggtgtaatta caaatctatt tttccattct aaaaacttat
     2341 aacatctgtc cttcataacg ttcaatccat ctgcatctaa tactattggg caattagaat
     2401 tttctactat cttaagtaat ttatcaaatg tttgactatt atctcccata cctggaccaa
     2461 aaccaattgc atcactagaa ttaagtaatt tatttaatct ttcttcttca aaatttaaag
     2521 tcattgcttc tgtaagtcta atagatacct tttcttgcac atcttcacta ctaattaatg
     2581 ttacaagtcc actcccactc ttaacagcag attcagttgc tatgaaactt gcaccataaa
     2641 atccttttga acctgcaact atagatactt ttccaaaatc acttttaaaa ccatgcttat
     2701 ctcttcttct aatattattt tttacaaaag tcttatcagt tatgaactct tcatcatggt
     2761 attttcttgt aataaaatct ggaacaccta tcttttctac aactacatca cctataagtg
     2821 cgctagtgtc atagtttaga aaacctcttt tataaaactc aaagctaatc gttttgtttg
     2881 ctctaataca agttcctaat acatttccat tatcgctatc tatacctgaa ggaatatcta
     2941 tagaatacgt atttttgcta ttatcgttta ctatgtcaat aacttcttta aaaataccag
     3001 ttatatcacg ttttaatcca gtcccaaata tagcatctac tacaacatca cttttagata
     3061 ctctatcttt aaaaattgtt aaatttttat tttttataat attttgtttc tcaactttaa
     3121 taattttatc tacttcattt tcctctccat aatttttttc acttaaatca tttttattaa
     3181 tatttataat attaattccc atatttaaaa gtacattgta atttatttta gagcattcac
     3241 ttagtttttc aagattacct actaaaaata cgtttacttc ttttccatat gtatttagat
     3301 gtcttgctat tccaagacca tctcctccat tatttccaac accacataca actgtataac
     3361 tattatacat gtctatatcc atatttttca ttgcagatat aaccgcattt tccatcaata
     3421 caatcattgg aatccctact tcttctacac atgcagtatc aacatttctg cttaatttag
     3481 atgtacctat tagcataaat tatcactcct ataaagttaa agacttctta aatacttacc
     3541 aaaaatatct attgaaatct ttttaatccc ataaatatca taaataaaat cactaaaact
     3601 aagagttttg aaataattta aaataacaaa aaattttgaa ttaaaacaat tatttaattt
     3661 attataccat ataatttaag ttattatgtt gatttaaaac ctatttacac taattatcta
     3721 aatatctctt tatttttata atcttttata acttatttat atttttcatt tttataaaaa
     3781 aacaataatt aagaatataa ctatattcta catgtaaaaa tttttttatt taaactcaat
     3841 tttctgataa tttgataatt tttcaaaatt tatagttgac ttttattttt tactgtatta
     3901 aactaattac tatacaaaac agaaaacaaa attttcaaaa ttattaaatt aaaaggagag
     3961 gtaaatatga agactttatt aaacaaacaa gtacgatttt ttagtagcca atttagttag
     4021 ggtttgtacc taagttaaga caaattgtca taacatagat acaagcccct taggattatg
     4081 atttttaagg attgtatcta tgttggctat agtaattgtt gttaaagttt ttattttatc
     4141 atcataatat agagccacat ggattatact gtgtggctta ttcgaatttt tgccatacag
     4201 ttatatatac tgtgtggttt ttttatatca aaatcatttt ttaaaaccaa attttaaaaa
     4261 ccatttaaaa tattttcaaa ggggtgtttt atttatgtca ggtattatat ctgtaatgac
     4321 gcaaagttta attttatcga ttatggcact gggggtttat ataacttaca aaatcttaga
     4381 ttttccagat atgtctgctg atggtagcta tacaatgggt gcttctattg tagcattttc
     4441 tcttactaat ggtatttctc ctgtagtagc aactttaatg gctatcttat gtggttgtac
     4501 tgctggtctt gttacaggga ttcttcacat taagtttaag atatctaatt tactttctgg
     4561 tattttagtt ctgggaatgc tatactctat caatttaaga ataatgggaa aatcaaatat
     4621 tcctctattt agctttaaac acttattcaa tggggaaata tctcctatag ttttagcctt
     4681 agcttttgta ttcatctgta aagtcctttt agatttattc ctaaaaacag gtcttggata
     4741 cactttaaaa ggtgttggtg ataactctca aatgattaaa tctctaggta taaatatagg
     4801 ttctataaaa atactaggac ttatgatttc aaa
//
LOCUS       DAMJVK010000176         4814 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.181, whole genome shotgun sequence.
ACCESSION   DAMJVK010000176 DAMJVK010000000
VERSION     DAMJVK010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4814
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.181"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            875..1537
                     /locus_tag="PC414_003683"
     CDS             875..1537
                     /locus_tag="PC414_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2466030.1"
                     /translation="MSILEIKNLNKIYGKSETEVKALSQINLKINSGEFVVIVGKSGS
                     GKSTLLHIMAGLETPTNGDVIIDNINISSLDEKKRSALRKEKLGLIFQSYNLIPVLTV
                     EENIKLPTINIKNKDEAYINELMELLGIKDRRMYLPNQLSGGQQQRVAIARALVNKPS
                     IVFADEPTGNLDTKTESDVLSLLKESQKKYNQTIIMITHNIDITKYADRVIEIEDGFI
                     RE"
     gene            1546..4053
                     /locus_tag="PC414_003684"
     CDS             1546..4053
                     /locus_tag="PC414_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2466031.1"
                     /translation="MKLNYIVKKYIMGNKKNTLLIIVSIVISTALFLIMNIISEDATN
                     LMINQAKNEFTLKHAQYINPKDEEIKYIENNTSIDKVGKSMLLGISDIGKGQTLQILS
                     QDKVAEELNDIYTLEKGKLPVAENEIAIDSWYIKQKKIKNPIGKRITLDYRRQGIDQE
                     DLYTGEKEFKITGILNSNPILKAQGTSIGLISNDCAIKNIPTKNKYDQVMFTFKKEKN
                     IQRQSQKLIKNGNLNENNIYFNNELLIAISDSMNLKIPYIIVNIVLALATILLIYNIF
                     YILVSNRIKDFGIFRALGFNPNDIFKIMILEVSIYSIISISIGLILGGIIASLSREYV
                     IGVIYNVNYINSIKNQNYINTYIISILLSLGTIIISVSKPLMLSVKTDPMICIRRNNE
                     KVDIKQNSFINKFMIKLFKDYGNIASKNIQRNKKRTNLSIASMVIIFFLMATIYTKST
                     SNFLSDGVLKYWIQGDYLLHNIDISTIRSNNKGYDKNVLQEIKAIDGVTKVNSYRHKW
                     FNIKIDDKSINKNSDYWKKNKDNIEARSEVKDGIKVYNNSFEFLGIEDTDILEDFLIN
                     GKENIDKFSKQPYLYITKKSSDSLNIKKGDRIKVYFDIIDSKTNNFTKTISQEFIVCG
                     IITTLPVTSQVGAEFGGVISTNQFNKFTGISSYERFDIWTSKLANDKYVESELNKITE
                     KTNKGILIPYKAETAEYEKIDNQKTLIMVLVTGIIVILSLFNCCNTIVTSINSRSREF
                     ALFRGIGISKHEINKIVKLESYIYIIVSFCISIIPILIVRSIIIKPFESINLINWKFI
                     GAIILIVFILVSIIMITTLKTLNKIQNENFMEEIKTL"
     gene            4384..4728
                     /locus_tag="PC414_003685"
     CDS             4384..4728
                     /locus_tag="PC414_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2466032.1"
                     /translation="MYCLSVVLAIYGIYVFINCTEYIYTMLSTKHLTVKGNEYTIINY
                     YMSNFSQYIIFAILLASMGFVIQKIVHMHFSEIELSGNYSTTVRNISDISLDNDNEMF
                     NRGNIENSILKK"
BASE COUNT         2126 a          422 c          706 g         1560 t
ORIGIN      
        1 taattgaata aattaaaaat ataatgaaag tttagtttta attcatttat ttaataaaca
       61 tttcaagtaa aataatgttt aaatcagaaa tataaataaa tattattata atcgttatta
      121 aagaccaaaa atcccacctt agaagtttgt atcatatgaa acctttatag attatgaaag
      181 caataatata gtgtatgata tataaaataa gttataaaag tattttttta gatattcaaa
      241 aagacattgt aagttatatt aataatatgg gtatcaacga attttggata taatgagttt
      301 ttcaacttaa agttatgtaa taatgaatgt aaagaatatt taatgaaatc attttgttgg
      361 ttatgtctag ctgaatacaa taatctttta gataaagatt ttatatttga agataggtgt
      421 gaaaaaatta agaagatatt aacatcagca gtttagaggc aaagcatgta aagtaaagtc
      481 tgggtaacta aaagtagtaa tttagattgt aactattgtt ataaatatga aaatgaggaa
      541 acaggaaata taagttagga tgtagaaaat aggttactaa ataaaaagct taattatata
      601 atcaaagaaa taggtatata ctgtacaatt tcatggtgaa gaacttttcc taaataaaaa
      661 ggttggtaaa agagatataa gaaataaaat tcaattataa gatatggtat gataactaaa
      721 ggcactatat ggagtgaata ggcggaagaa ttttttgaaa attataaaaa tagtttctgt
      781 tgctacatat caataagtat agatagagat tgcaaaatcc atagcaaaaa tcgaatttat
      841 aagtgtaaat aacatgtaag acaaggagta aattatgagt attttagaga taaaaaacct
      901 aaataaaata tatggtaaat cggaaacaga ggtgaaagcg ttaagtcaaa taaatctaaa
      961 aataaatagt ggagaatttg ttgtgatagt aggtaaaagt ggaagtggga aaagtacttt
     1021 actacacata atggcaggac ttgaaactcc aacaaatggt gatgtgatta ttgataatat
     1081 aaatatatca tctcttgatg aaaagaaaag atctgcatta cgtaaagaaa agttgggatt
     1141 aatatttcaa tcatataatt tgattccagt tttaactgtt gaagaaaata taaaactacc
     1201 aactataaat ataaaaaata aagatgaagc ttatattaat gaattgatgg agttactagg
     1261 aataaaagat agaagaatgt atttgccaaa tcaattatca ggtgggcaac aacaaagagt
     1321 agcaatagca agggctcttg taaataaacc atcaattgta tttgctgatg aacctactgg
     1381 aaatttagac acaaagacag agtcagatgt attaagttta ttaaaagaat ctcaaaaaaa
     1441 atataatcag acaataataa tgataacaca taatatagat ataacaaaat atgctgatag
     1501 agttatagaa atagaagatg gatttataag agagtaggta gatttatgaa acttaattat
     1561 attgtaaaaa aatatataat gggaaataag aaaaatacat tattaataat tgtaagtatt
     1621 gtaatttcaa cagcgttgtt tttaattatg aatataataa gtgaagatgc gacaaactta
     1681 atgataaatc aagctaaaaa tgaatttaca ttaaaacatg cacaatatat caatccaaaa
     1741 gacgaagaaa taaaatatat agaaaataac acaagtatag ataaagttgg aaaatcaatg
     1801 cttttaggaa ttagtgatat tggtaaaggt caaacactac aaattctatc tcaggataaa
     1861 gttgctgaag aactaaatga tatttacaca ctagaaaaag gtaaattacc agtagctgaa
     1921 aatgaaatcg ctatagattc atggtatatt aaacaaaaaa agataaaaaa ccctatagga
     1981 aaaaggataa cattagatta tagaagacaa gggatagatc aagaagattt atatactggg
     2041 gaaaaagaat ttaaaataac tggtatctta aatagtaatc ctattttaaa agctcaagga
     2101 acatcaattg gccttataag taacgattgt gctatcaaga atattcctac taaaaataaa
     2161 tatgatcaag ttatgtttac ttttaaaaag gaaaaaaata tacagagaca atcacaaaaa
     2221 cttattaaaa atggaaattt gaatgagaat aacatttatt ttaataatga attattaata
     2281 gctatatctg atagtatgaa tttaaaaata ccttatataa tagttaatat agtactagca
     2341 ctagcaacta tattattaat ttataatatt ttttatatat tagtatcaaa tagaataaag
     2401 gattttggga tatttagggc tttagggttt aatccaaatg atatttttaa aataatgatt
     2461 ttagaagtat ctatatactc tattataagt atttctatag gtctaatttt aggtgggata
     2521 atagctagtt tatcaaggga gtatgttata ggagtaatat acaatgtcaa ctatataaat
     2581 tctataaaaa accagaacta tataaacaca tacataattt ctatattatt aagtttagga
     2641 actataataa tatcagtttc aaaacctctt atgttatcag ttaaaactga tccaatgatt
     2701 tgtataagaa gaaataatga aaaagtggat ataaaacaaa attcctttat aaataaattt
     2761 atgataaaac ttttcaaaga ctatggtaac atagcatcaa aaaatataca aagaaataag
     2821 aaacgtacaa atttatctat agcatcaatg gtaattatat ttttcttaat ggcaacaata
     2881 tacacaaagt ctactagtaa ctttttaagt gatggtgtat taaaatattg gatacaagga
     2941 gattatttat tacataatat tgatataagt actatacgtt caaataataa aggttatgat
     3001 aaaaatgtac tacaagagat aaaggcaatt gatggtgtaa caaaagtaaa ctcatatagg
     3061 cataagtggt ttaatataaa aattgatgat aaaagtataa ataagaactc tgattattgg
     3121 aaaaaaaata aagataatat tgaagctaga tcagaagtaa aagatggaat taaggtatat
     3181 aataattctt ttgaattttt aggaatagaa gatacagata tactagagga tttcttgata
     3241 aatggaaagg aaaatataga taaatttagt aaacaaccat atctgtatat aacaaagaaa
     3301 tccagtgatt cgcttaatat aaaaaaagga gatagaataa aagtatactt tgatattata
     3361 gattcaaaaa caaataattt taccaaaaca atatctcaag aatttatagt atgtggaatt
     3421 ataactacat taccagtaac ctcacaagtg ggagctgaat ttggcggagt aatatcaaca
     3481 aatcaattta ataagtttac aggaatatca tcttatgaaa gatttgacat atggacgagt
     3541 aaactagcca atgataaata tgttgaaagt gagttaaaca aaataacaga aaaaacaaat
     3601 aaaggaattt taattccata caaagctgaa acagctgaat atgaaaaaat tgataatcaa
     3661 aaaacactaa ttatggtatt agtaactgga attatagtta tattatcttt atttaattgt
     3721 tgcaatacta tagttactag tattaatagc agaagtaggg aatttgcact atttagagga
     3781 ataggaatat caaaacatga aataaataaa attgttaaat tggaaagtta tatatatatt
     3841 atagtaagct tttgtatatc tataatacct attttaattg taagaagtat aataataaaa
     3901 ccttttgaaa gtatcaattt aataaattgg aaatttatag gggctattat actaatagta
     3961 tttattttag tatctataat catgataact acactaaaaa cattgaataa aattcaaaat
     4021 gaaaatttta tggaagaaat aaagacttta tagtgaacag cctataaatt aaagaggttt
     4081 tatatgaatt taattgcaat tatcgataat ggtgtagata ttgattttaa ataatttaga
     4141 aataaaaata aaataagtta tccaagttat atgtatgatg taataggggt acaatgtata
     4201 gcaaatataa atattggctt aataaaactc tactcttaag tctactgtcg ttttaacaca
     4261 agactaataa taagtgtaac ctaaaaatta ttgataatac aataaccttg tttatgataa
     4321 ataacaaata ttaagtgttg taaagatagt aagtttaaaa aaatatcaaa gatttcaatc
     4381 acaatgtatt gtttatctgt agtgttagct atttatggta tatatgtatt tataaattgt
     4441 actgaatata tatatactat gctatctaca aaacatttaa cagtcaaagg aaatgaatat
     4501 accattataa attattatat gagtaacttt agtcagtata taatatttgc tattttactt
     4561 gcatcaatgg gatttgtaat acaaaagata gtgcatatgc atttcagtga gatagaactt
     4621 agtggtaact attcaacaac tgttagaaat ataagtgata tctctttaga taatgataat
     4681 gaaatgttta atagaggcaa tattgaaaat agtattttaa aaaaataaat aatataaata
     4741 tataaaatgt gattgaataa agcttagaat acttagtgta ttttaagctt tttttattat
     4801 taagatttca aagt
//
LOCUS       DAMJVK010000177         4778 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.182, whole genome shotgun sequence.
ACCESSION   DAMJVK010000177 DAMJVK010000000
VERSION     DAMJVK010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4778
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.182"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            200..1351
                     /locus_tag="PC414_003686"
     CDS             200..1351
                     /locus_tag="PC414_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDF2466033.1"
                     /translation="MEIYLDNSATTKPYQEVIDKMVYALNTEYGNPSSVHRKGVEVEK
                     AIKEVRQDIAKSLGAKEKEIYFTSGGTECNNTIIRGITSLNKKRKNHIISTNIEHPSV
                     LNTLKDLEEDGFEVTYLEVGKDGKINIEDLKNAIKSTTCLVSMMHVNNEIGTIQPIGE
                     VGKYLKGLKEKIYFHVDAIQSYGKINFRPSKYNIDFMSVSAHKFHGPKGIGFMYIKEN
                     NRLKPMLTGGGQEIGIRSGTENVPGIYGLGEAVRILNKDLDAVISKVDNLKNILKNEI
                     IDNIEDIKINSPEDGVCHILNVSFRGTKGEVLLHYLEQKGIYVSTGSACSSKKKGSYV
                     LNAIGLTNEEINGTIRFSLSDMNTEEEMLEAVKVLKESICDLRSIMKRK"
     gene            1397..2572
                     /gene="thiI"
                     /locus_tag="PC414_003687"
     CDS             1397..2572
                     /gene="thiI"
                     /locus_tag="PC414_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HDF2466034.1"
                     /translation="MVYNILIVKYGEIGVKGKNRYIFENRLIRNIRNMLKPIGRFNVY
                     KEYGRIYVDLEDYDYEEVIEEVRKVFGIVGVCPGVRAKKDYDTLKEIALKMLEEKIEA
                     GYKTFKVESRRGDKSFGLTSQEMSMDIGGYLLSKVGDRINVDVRNPEVKIKCEYREFH
                     TMVYSDTIPGYGGLPLGTNGRAMSLLSGGIDSPVATWMVAKRGMEVEAVHFHSYPFTS
                     ERSQEKVKDLAKILAKYCGRVRLHKVNILEIQKAIGENCNEEESTILSRRFMMRIAQR
                     LSEKRHCDALITGESIGQVASQTIQGLTCTNAVVDLPVFRPLIAMDKSDIVDIAKKIG
                     TFETSIVPEEDCCSVFSPRKPVTKPRLEKIEKSETALDIEKLVQDAIDKIEVEDIEF"
     gene            2728..3531
                     /locus_tag="PC414_003688"
     CDS             2728..3531
                     /locus_tag="PC414_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDF2466035.1"
                     /translation="MNKKGFTLIELLVVISIIGILVIVAVPALFRNIEKSKAVTCLSN
                     RENIKTQIVIAMAEESSKGKNEVMKEVLENKDGKYFETEPKCKSGGIYSATFDDGYDG
                     ITGIESIAKVYVTCTKHPDGVEMARDIHQSMKDLIASFAQDPSIIPGASKGNDDFRKY
                     LLDNKYKNGWPTIPDEFKAKYGLSKDTLYIQPYAYNPTKSDATVVVFANNKTGGNWYT
                     SLVYDYDEGRWYKGKNGISVAGRSWDVDTDSVKSVKTEIHSKEGWGPLN"
     gene            3774..4415
                     /locus_tag="PC414_003689"
     CDS             3774..4415
                     /locus_tag="PC414_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HDF2466036.1"
                     /translation="MNNNFQDNQTINLIQSRRSIRKFTTEQISDEQVNTLLHCAFAAP
                     SGCNKQPWHITVVQDQKLLKEISDDTLSRIHEVSNVEINKNFKLFYGAPTVLFISYDE
                     SSSWAPYDIGILTGNITTAAQALGLGSCIIGMVRGLFTPVEQGDIEGLVSVLDKEDVK
                     ESESIKMKFDTNKKYRELLDIPEGYSVPFGIAVGIPDGNLPNAREVVYKVSRV"
BASE COUNT         1918 a          454 c          924 g         1482 t
ORIGIN      
        1 tatatttttg tagggtatag tataagtata ttatatttta ctataaaaat ttgtaataaa
       61 gtgaaaaaac tagcttttca tgaaataata ttttataatt taagattgtc tttttaataa
      121 agattaacaa aaaaaatact ataattagtg gtataatagt tagagttgaa gattttaaat
      181 aaaggtaggt gtaattaagt tggaaatata cttagacaat agcgcaacta caaaacctta
      241 tcaagaagtt atagataaaa tggtttatgc acttaatacg gaatatggaa atccttcttc
      301 tgtccataga aaaggtgtag aagttgaaaa agctataaaa gaagtaagac aagatatagc
      361 taaatctcta ggagcgaaag aaaaggagat ttattttaca tcaggtggta cagagtgtaa
      421 taatactata ataagaggta ttactagttt aaataaaaaa agaaaaaatc acataatatc
      481 tactaacata gaacatcctt ctgttttaaa tacattaaaa gatttagaag aagatggttt
      541 tgaagtaact tatttagagg tcggaaaaga tgggaaaata aatatagagg atttaaaaaa
      601 tgcgataaag tcaactacat gcttagtaag catgatgcat gttaacaatg aaataggaac
      661 tatacagcca ataggtgaag ttggaaagta tttgaagggt ttaaaagaaa aaatttattt
      721 ccatgttgat gctattcagt catatggaaa aattaacttt agaccatcta aatacaatat
      781 agattttatg agtgtaagtg cacataaatt tcatggtcca aagggaattg gatttatgta
      841 tataaaagaa aataatcgcc ttaagcctat gttaacaggt ggtggtcagg aaattggaat
      901 taggtcggga acagaaaatg tacctggaat atatggtctt ggagaagctg ttagaatact
      961 aaacaaagat ttagatgctg ttatttcgaa agtagataac ttaaaaaata tattaaaaaa
     1021 tgaaataatt gataatatag aagatataaa aatcaattct ccagaagacg gagtatgtca
     1081 tatattaaat gtatcattta gagggacaaa aggagaagtt ttacttcatt acttagagca
     1141 aaaaggtatt tatgtatcaa ctggctctgc atgttcttct aaaaagaaag gtagttacgt
     1201 attaaatgct ataggtctta caaatgaaga aataaatgga acaataagat ttagtttatc
     1261 tgatatgaat acagaagaag aaatgttaga agctgtgaaa gttttgaaag aatcaatttg
     1321 tgacttaagg tcaataatga aaagaaaata aagaatactg tataaatagt gataataaat
     1381 tagtttaaga ggtgaaatgg tgtataatat attaatagta aagtatggag aaattggagt
     1441 aaaaggaaag aatagatata tatttgagaa taggcttata agaaatataa gaaatatgtt
     1501 aaaaccaatt ggaagattta atgtatataa agagtatgga agaatatatg ttgatttaga
     1561 agactatgat tatgaagaag taattgaaga agttagaaaa gtatttggga tagtgggtgt
     1621 gtgccctgga gtaagagcta aaaaagatta tgatacatta aaagaaatag ctttaaaaat
     1681 gttagaagaa aaaatagagg ctggatataa aacttttaag gtagagtcaa gaagaggaga
     1741 taagtctttc ggattgactt ctcaagaaat gagtatggat ataggtggat acttattatc
     1801 aaaggtagga gataggatta atgttgatgt aagaaatcca gaagttaaaa taaagtgcga
     1861 atacagagaa tttcatacta tggtctatag tgatactata cctggatatg gtggattgcc
     1921 acttggaacg aatggtagag ctatgtctct tttatcagga ggtatagata gcccagtagc
     1981 tacatggatg gttgcaaaaa gaggtatgga agtagaggca gttcactttc atagttatcc
     2041 atttacaagt gaaaggtcac aggaaaaagt taaggattta gctaaaatac tggcaaaata
     2101 ctgtggtaga gtaaggcttc ataaagttaa tatcttagaa atacaaaaag caattggcga
     2161 gaattgtaat gaagaagaat ctactattct ttcaagaaga tttatgatga ggatagcgca
     2221 gagactttct gaaaaaagac attgtgatgc tttaattaca ggtgaaagta ttgggcaggt
     2281 tgcatctcaa actatacaag gacttacttg tactaatgcc gttgtagact taccagtgtt
     2341 tagaccactt atagcaatgg ataaatcaga tatagttgat atagccaaaa aaatagggac
     2401 ttttgaaaca tctatagtgc ctgaagaaga ttgttgtagt gtattttctc ctagaaagcc
     2461 cgtcactaaa cctagattag agaaaataga aaaatctgaa acagcacttg acattgaaaa
     2521 attagtacag gatgctattg acaaaataga ggtagaggat attgagtttt agcatacaag
     2581 ttatttaatt actaaattga atattgagta tattagtagt tctgtaagta tattagtagt
     2641 tctgttgaaa ggatttatgt agacaattta ttaatatgta gtatttaaaa ataagtacta
     2701 taattttatt taatgggaat gattattatg aataaaaagg gttttacact aattgaattg
     2761 ttagtagtta tatctataat aggcatttta gttatagtag ctgttccagc attgtttaga
     2821 aatatagaaa aaagtaaagc agttacatgt ctttctaata gagaaaatat aaagactcaa
     2881 attgttattg caatggctga ggaatcaagt aaaggcaaga atgaagtcat gaaagaggta
     2941 ttagaaaaca aagatggtaa gtactttgaa acagaaccaa agtgtaagtc aggtggaata
     3001 tattcagcaa cgtttgatga tggttatgat ggaataactg gaatagaaag cattgcaaaa
     3061 gtgtatgtta cttgtacaaa acatccagat ggtgttgaaa tggctaggga tatacatcaa
     3121 agtatgaaag atttgattgc atcatttgca caagaccctt ctataatacc aggagcctca
     3181 aagggtaatg atgattttag aaaatattta ttagacaata aatataaaaa tgggtggcct
     3241 acaattccag atgaatttaa ggcaaaatat ggattaagta aggatacact atatatacaa
     3301 ccatatgcat ataatcctac taaatctgat gctactgtag ttgtatttgc aaataataag
     3361 actgggggta attggtatac ttccctagtt tacgattatg atgaaggtag atggtataaa
     3421 ggtaaaaatg gtatttctgt tgcaggtagg tcatgggatg ttgacacaga tagtgttaag
     3481 tctgtaaaaa cagagattca ttctaaagag ggatggggtc ctttaaatta atatatcatg
     3541 ttttattaat ctggtattga ttaaactata gaaaagagca gtttctatag atgacgaata
     3601 ccagattttc atttatatat ataattgaga tatattaaaa gtgtttatat ttttaagtct
     3661 tcacatacaa gctaaaaata attataataa tattgtaaag aaatttaatt ataagattaa
     3721 ttttctgaag atttagtatt tgtactaaga aatacataaa aaggggaata gatatgaata
     3781 ataattttca agacaaccaa acaataaatt taatacaaag tcgtagaagt atacgaaaat
     3841 ttacgacaga acaaattagt gatgagcaag taaacacatt attacactgt gcatttgcag
     3901 caccttcagg atgtaataaa cagccttggc acataactgt tgtgcaagac cagaaacttt
     3961 taaaagagat tagtgatgat actttatcta ggattcatga ggtgagtaat gttgagataa
     4021 ataaaaactt taagttattt tatggtgctc caaccgtttt atttatttct tatgatgaaa
     4081 gtagttcatg ggctccatat gatataggaa tacttacagg gaatataaca acagctgcac
     4141 aagcactagg gcttggaagt tgtataatag gaatggttag aggtttattt acacctgttg
     4201 aacaagggga tattgaaggt cttgtgagtg tattggataa agaagatgta aaagaatcag
     4261 agtcaataaa gatgaagttt gatacaaata aaaaatacag agaactactt gatataccag
     4321 aagggtattc tgtaccattt ggtattgctg taggtattcc agatggaaat ttaccaaatg
     4381 ctagagaggt agtttacaaa gtttccagag tttaggataa agtattgcaa gaaccaatca
     4441 gttacttgaa gaattaaatc agtaatatac aaataaattg atggaagagg aagctattta
     4501 aaaagtagtt tagatttttc gttgtaaaat aattaaataa agaataaggt gataagtatt
     4561 ttatttatta tcttattctt ttttattttt gttttgtaga atgataaaaa taaggattaa
     4621 gtgtaataaa atagaaaaca agtatgttaa ttttaagtat atgctttaaa atttatccaa
     4681 gttaaaaatt taagatatct tttcagtata atggaaaaaa tgtcaaaata ttgaaatagg
     4741 gaaaaaatag tgatataata agaaaaaata gagaaaaa
//
LOCUS       DAMJVK010000178         4761 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.183, whole genome shotgun sequence.
ACCESSION   DAMJVK010000178 DAMJVK010000000
VERSION     DAMJVK010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4761
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.183"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(23..787)
                     /locus_tag="PC414_003690"
     CDS             complement(23..787)
                     /locus_tag="PC414_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDF2466037.1"
                     /translation="MFAEERIQAILNILKKEGRVTVKELSSKFNVTEDCIRKDLKNIE
                     KISSIRRIYGGAVLARETLENQDTKDRKEINIPTKKIIAEKAFNLIEDRETIFLDIST
                     INILLAKLLAESNKKVTLVTNMLDILNVVTSKPNNLNIISTGGLLNLSLDGFVGTPTI
                     EFISKYKFDKTFMGSCGVDVFNSSLTTFEIEDGLTKKAIINSSKKVFIVMEDKKFKFD
                     GNFKFAHLEDISSIITEKTPTPDIVDILSEFNVNVL"
     gene            1061..1735
                     /gene="fsa"
                     /locus_tag="PC414_003691"
     CDS             1061..1735
                     /gene="fsa"
                     /locus_tag="PC414_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDF2466038.1"
                     /translation="MQLILDTGNVEEIKELCTCLPIDGVTTNPSIVSKEKKNFKQLIN
                     EIGEIIGEDMPIHAQVLSTKYEEILEEALYISSLRKNIYVKIPVTQDGLRAIKDLHRK
                     GVKITATAIFTAHQGFLAAKAGANYIAPYVNRLDNISGDGVAMVSELIKIIDTYKMDT
                     KVLAASFKNAQQVIELMQHGVHSVTVPYDICKSMMNHPLTDWSVGKFIEDWENTFGKG
                     SKTNNI"
     gene            complement(1857..3251)
                     /locus_tag="PC414_003692"
     CDS             complement(1857..3251)
                     /locus_tag="PC414_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HDF2466039.1"
                     /translation="MGIILKPKQFYMTVIATIILYFSLNYSFNIKFSICLSFLTILIW
                     AVDSVEKTFVALFFVILAFIFNIAPIKVILQFLFTENFYIIILAYIITNAVTKTGVAK
                     IISEKLILNTVNTPKKMILLSYILGILLIFFIPQPFPRVILVSAFYKEFLKEQQITED
                     SKSILLFSIFTASTFTSMFFVNGDTLLNYVVLELGNCNINWGQWAFYMSVPTIITCFI
                     TYFLFIFVFKKELHSIKFISNENLEGNKLHFSYSNLKKEFNLLNKEQKHVFLCLGIMF
                     FMFLTQFIHNINTLIIMSICVLLLLLRKIVGFSTLKEINWKVLIFFIAAFSIGGVLKY
                     SGVVDIMGNYLIKFIPNSSEIISILFLITLTIILNICLGSAVTTSSVVIPLLGSLHIL
                     KENSVTLCLFVYIIVSIQYILPFHHATIMVGHGENLYNSKVIFKYGLTLIPLTYFIII
                     CITFPWWKFIGLEI"
     gene            complement(3329..4675)
                     /locus_tag="PC414_003693"
     CDS             complement(3329..4675)
                     /locus_tag="PC414_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2466040.1"
                     /translation="MKDLTTGHEGKSIFFFAMPMLIGSLFQQLYNTADSIIVGRFIGK
                     EAMAAVSGANPIMFLLVAALMGVSLGFSILVSQFYGSGDLKKVKATIDTTYILLFIGS
                     ILISILGIVFGGPMLKLMNTPESVFAQSKLYLTIIFSGILFSAGYNSVSAILRGLGDS
                     VTPLYFLIIATILNIVLDLTFIVVLRMGVEGVALATIMAQAVSFIISIIYLNKKHEVL
                     KFKIKGIVYDNKIFKDGLRLGLPSGVQQMLFSIGNMALQFLVNSYGTSAMAAFGAGLR
                     IENFISLPIMNLGSAVSTFVAQNIGAGENERVKKGIRESIKMTLVLAVTVIALILLFR
                     ENLIALFNTDKDVIKIGSSYLFIIGPFFLFIGTSFVLSSAMKGAGDSMFALISSIVSL
                     WLGRLPASYMLSKFFGTDGIWMGIPFGWTLGLIVTVIYYKKGHWKTKAIVNHRINE"
BASE COUNT         1841 a          674 c          623 g         1623 t
ORIGIN      
        1 cctataaaac atataattaa acttataaaa catttacatt aaattctgat aaaatatcta
       61 ctatatcagg agtaggcgtt ttttctgtaa ttatagaact aatatcttct aaatgagcaa
      121 acttgaaatt tccatcaaat ttaaactttt tatcttccat aactataaat acttttttac
      181 ttgaatttat tatagccttc ttcgtaagac catcttcaat ttcaaaagtt gtaagtgaag
      241 agttgaatac atctactcca caacttccca taaaagtctt atcaaactta tatttcgata
      301 taaactctat agttggagta ccgacaaaac cgtctaaact gagatttaat aaacctccag
      361 tggaaattat atttaaatta tttggttttg aagtaactac atttagtata tccagcatat
      421 ttgtaacaag tgttactttt ttattacttt cagctaacaa cttcgcaagc aagatgttaa
      481 ttgtagagat atctaaaaat atagtttctc tatcttcaat taaattaaat gctttttctg
      541 ctattatttt ctttgttgga atattaattt cttttctgtc ttttgtatcc tgattctcta
      601 gtgtttctct agctagtaca gctcctccat aaattcttct tattgaagaa atcttttcta
      661 tattttttaa atcttttctt atacaatctt ctgtaacatt aaatttagaa ctgagctctt
      721 tgacagtaac tctaccttct ttttttagga tatttaatat agcttggatt ctttcttctg
      781 caaacatttt ctacctcttt tcgcaatata gtattatagt aaatattata agataaaata
      841 caatatatca actattttaa catgaaatac aaaattaatt taatataaaa agtatgtttt
      901 ttctaactat atatttagaa tttagtaaat aaaaaaatta taaaactaag aaggaaaagt
      961 aaaacaataa taaaaaataa aaaacaataa gcaaaatgtt gaaaataata ccaaatcaat
     1021 atataataag aataaagaaa gattaaaggg ggatatttta atgcaactaa tcttagatac
     1081 aggtaatgtg gaggagatta aggagttatg tacttgttta ccaatagatg gtgtaactac
     1141 aaatccaagt attgtttcaa aagaaaaaaa gaattttaag caacttataa atgaaattgg
     1201 tgagattata ggtgaggata tgcctataca tgctcaagtg ttaagtacaa agtatgaaga
     1261 aatattagaa gaagcattat atataagtag tttgagaaaa aatatatatg taaaaatacc
     1321 tgtaacacaa gatggtttaa gagctataaa agaccttcac agaaaaggtg taaaaataac
     1381 tgctactgca atattcactg ctcatcaagg ttttttagct gcaaaggctg gagcaaatta
     1441 tattgcacct tatgtaaata ggcttgataa tatatctggt gatggagtag ctatggtttc
     1501 agaacttata aaaataatag atacttataa aatggataca aaagtacttg cagccagttt
     1561 taaaaatgca caacaagtta tagaattaat gcaacatggg gtgcatagtg ttacagtacc
     1621 atatgatatt tgtaaatcta tgatgaatca tccattaact gattggagtg taggtaagtt
     1681 tatagaggat tgggagaata cttttggaaa aggaagtaaa acaaataata tttagtatta
     1741 tggtaaaaga gtagttttgt ccaatttcac aagaacttat ataaaaagat gaaaatatga
     1801 attttctttt taataatttc gttattatct taagttaatt catattttca tcttttttag
     1861 atttctagtc ctataaattt ccaccaaggg aatgttatac atattattat aaagtaggtt
     1921 aaaggaatca aagttaaacc gtatttgaaa ataaccttgc tattatacag attttcacca
     1981 tgaccaacca ttattgtagc atgatgaaat ggtaatatgt actgtatgct tactataatg
     2041 tagacaaata gacataaagt aacactattt tcctttagta tatgtaaact tccaagtaac
     2101 ggtattacta cagatgatgt agtgacagca cttccaagac atatattcaa gattattgtg
     2161 agagttatca aaaacaatat tgaaataatt tctgaagagt ttggtataaa ctttatcaaa
     2221 taatttccca ttatatctac aactccagag tactttaaca ctcctccaat tgaaaatgct
     2281 gctataaaaa aaataaggac cttccaattt atttctttta aagtactaaa tccaactatt
     2341 tttcttagta ataaaagaag tacacaaatt gacattataa ttaatgtgtt tatgttatgt
     2401 atgaactgag ttaaaaacat aaaaaacatt attcctaggc ataaaaatac gtgtttttgc
     2461 tctttgttta gcaaattaaa ttcttttttt aaattagaat atgaaaaatg aagtttatta
     2521 ccctcaagat tttcattaga aataaatttt attgaatgca attctttttt aaagacaaag
     2581 ataaataaaa aataagttat aaaacaagta attatagttg gaacagacat ataaaaagcc
     2641 cattgacccc aatttatatt acaatttcca agttctagta caacataatt tagaagggtg
     2701 tctccattta caaaaaacat tgatgtaaag gtagatgctg taaatatact aaataaaagt
     2761 atagattttg aatcttcagt tatctgttgt tcttttaaaa actccttgta aaaagcagat
     2821 acaagaataa cccttggaaa tggttgtggg ataaaaaata ttaaaagtat acctaaaata
     2881 tacgataaaa gaatcatttt tttaggggta tttacagtat ttaaaattaa cttctcagaa
     2941 ataatttttg cgacacctgt ttttgtaact gcattagtaa ttatgtatgc aagaattatt
     3001 atgtaaaaat tttctgtaaa taaaaactgt aaaataacct ttattggtgc aatgttaaat
     3061 ataaacgcta gaattacaaa aaacaatgca acaaaagttt tttcaactga gtctacagcc
     3121 catatcaaga tagttaaaaa agataagcat atagaaaatt ttatgttgaa actgtaatta
     3181 agagagaagt agagaattat ggtagctata acagtcatat aaaattgttt tggcttaaga
     3241 ataataccca tttttatatt cacctccata atttattata tcataatgga ggtgaataca
     3301 atgaatgtga atacatttta taaaaagatt attcattgat tctatgattt acaatagctt
     3361 tagttttcca atgtcctttc ttatagtaga taactgtaac aataagacct aaagtccaac
     3421 caaaaggaat acccatccaa attccgtctg ttccaaaaaa tttagaaagc atataagaag
     3481 ctggaagtct tccaagccat aaagatacta ttgagcttat aagtgcaaac atggaatcac
     3541 cagcaccttt cattgcactt gaaagtacaa aagaagttcc aataaataag aagaatggtc
     3601 ctattataaa taagtaagaa gagccaattt taatgacatc tttatcagta ttgaatagag
     3661 caattagatt ttccctaaat agcaaaatta aagcaataac tgtaactgct aaaactaagg
     3721 tcatttttat agattctcga atgccttttt taactctttc attttctcca gcacctatgt
     3781 tttgagcaac aaaagtagaa acagcagaac caagattcat aataggtaag cttataaaat
     3841 tttctatcct aagaccagca ccaaaagctg ccatagcaga tgtaccataa ctattaacta
     3901 aaaattgaag agccatattt ccaattgaaa acaacatttg ttgaactcca cttggaagtc
     3961 ctaatctaag tccatcttta aatattttgt tatcatatac aattcctttg attttaaatt
     4021 taagcacttc atgtttttta tttaaataaa ttatacttat tataaatgat actgcctgag
     4081 ccattatagt ggcaagtgca acgccctcaa cacccattct tagcactaca ataaaagtta
     4141 agtctaaaac tatatttaga attgtggcta ttatcagaaa ataaagtgga gttactgaat
     4201 ctccaagtcc tctaagtatt gcacttactg aattatatcc agcagaaaat aatattccac
     4261 taaatattat agtaagatac aattttgatt gagcaaaaac ggattctggt gtattcataa
     4321 gcttaagcat tgggccacca aatactattc caagaatact tattaatata gaaccaataa
     4381 ataaaagtat atatgtagta tcaatagtag cttttacctt ttttaaatct cctgagccat
     4441 aaaattgtga cactaaaata gaaaatccaa ggctaacacc cataagcgct gccaccaata
     4501 aaaacattat agggttagct ccagagactg ccgccattgc ttcttttccg ataaatctac
     4561 caactattat actgtcagct gtattgtata gttgttgaaa taaactacca ataagcatag
     4621 gcatggcaaa aaagaatatg gactttccct catgacctgt agttaaatct ttcaccttat
     4681 tcctccaaat aaaaaaatta ttaatttatt atagtataat cttaacataa actttatatc
     4741 caaactataa ataaattgta t
//
LOCUS       DAMJVK010000179         4637 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.184, whole genome shotgun sequence.
ACCESSION   DAMJVK010000179 DAMJVK010000000
VERSION     DAMJVK010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4637
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.184"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            595..1902
                     /locus_tag="PC414_003694"
     CDS             595..1902
                     /locus_tag="PC414_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2466041.1"
                     /translation="MKEIADAITSEYLSYGINEDISIYEGRFCIYLDKKYRCNGKIYY
                     KMTPPISISFKADIGCVEEIDNEDDNLALDYDNAILEVHGYKIISITINTLSEFSVEG
                     YINDDCIKSKNSYVEYVDFNIINLDKIPGKLIKYNDKVYAGRIEFDINDYVVTIDKRY
                     DYRKELKSELKSKSGAIITHIGRIRRKDGRIFRTNNTINLLDRISTALSFMCGRYVGF
                     CLAKGYRSGNEVYRIWNENQISPFRYVPTWSDTLSNYHNMEKYISLMCKKLEDFYYGS
                     AIKSVVDWYIESLGSATMENNIISVQIALETLSYVILVEQNKILTDEAFDCNLASKNI
                     RLLLDTCKIPYGKHELNIFDNIIKNKFDDGVDLVIYLRNSIVHPSRKTHRAVLEVEDI
                     WNIISIGTRYIELVLLFILGYRGEYSNRLVERCYGEVEVVPWN"
     misc_binding    1988..2250
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2376..3179
                     /locus_tag="PC414_003695"
     CDS             2376..3179
                     /locus_tag="PC414_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2466042.1"
                     /translation="MKNILKKVGIFTIMLGLLGGVVGCSKPDNEKDKDASKESKKEVV
                     VGFDNTFVPMGFLDEKGDTVGFDVDLAKETFKRLGMEVKFQPIDWSMKETELNDSKTV
                     DVLWNGYSITDERKKIVSYTEPYLQNKQIIVTLSDSKINSKADLKDKEVGTQQGSTAL
                     DAVEKDKDFMNSLKGGAPVLYDTYDKALRDLEIGRTSAVVGDEVLIRYYMGQKGEDKY
                     KVLKDDFGLEDYVVATSKENPELCEKINETLKEMKKDGTFDKIYDKWFK"
     gene            3217..3828
                     /locus_tag="PC414_003696"
     CDS             3217..3828
                     /locus_tag="PC414_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDF2466043.1"
                     /translation="MLQGLEIVIAMFCITLIVSIPLGIGVAFLRLSKNKLVSGITQCY
                     ILIMRGTPLLLQMIVIFYGLPLLGIVFDRFTAGVVAFFLNYAAYFAEIFRGGIQSIDR
                     GQYEASKVLGFDKFTMYKRVIFPQVFKRILAPISNEVITLVKDTSLVYILGLNDILRI
                     SQIAMNREASLLPLFEAGAIYLIFVAILTKGFELLEKKYSYYR"
     gene            3860..4531
                     /locus_tag="PC414_003697"
     CDS             3860..4531
                     /locus_tag="PC414_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDF2466044.1"
                     /translation="MLKIKNLNKSFKKNRVLKDISFELEEGQIGVLLGKSGAGKTTIL
                     RCINGLEEFDSGEIIIDNEVIKNKRDMAKIRGKIGMVFQNFNLFPHMTVLENIIESPV
                     NVFKVPRKEAEERARELLRLVDLEDKLNSYPFELSGGQQQRVAIARSCALMPKVLCFD
                     EPTSALDIDTIQRVVNIMNRLKDKGMTILIITHDVVFSNNVADKIISIKDGIVENVQI
                     KEKIV"
BASE COUNT         1863 a          426 c          830 g         1518 t
ORIGIN      
        1 gataataaaa caacaatggt gctaatttat atgtataaat taattatgaa aaatttatat
       61 aagtaaaata tattaacata aaggtaaaat tttgtatact aaaagggaat atttacagtt
      121 taatggcagt tttaaaataa taaattaaag ctgtcatttt tatttaaaaa tttttagaaa
      181 aagtacaata gtgttaatgt gtgaattaga taaggtgtat ataatagttt agatgatatg
      241 atataaaata tgttgtaaat taaatgtttt ctttgacaat atatacacaa ggatgataat
      301 atctatatga ataaaagata gtgtgtaatt tgataatatg taattataac attatgatat
      361 aattacaata aagtatgtaa taatctgtag aaatttcaag ctagttatct aagttttcgg
      421 ataactaaat ctaaacggaa tacataatgt attaaataaa tgatgatact aaaatgatac
      481 tatttttata gaaatttatt gaggtatata attaatgaaa ataatatatt aaattatata
      541 aattaaatta tataatatat tgagttttac ataaaagcaa aggtgggaat aaaaatgaaa
      601 gaaattgctg atgctataac atcagagtat ttaagctatg gtatcaatga agatattagt
      661 atctatgaag gtagattttg catatattta gataaaaaat atagatgtaa tgggaaaatt
      721 tattataaaa tgacacctcc aatatctata agttttaaag cagacatagg ttgtgttgaa
      781 gagatagaca atgaagatga caatttagct ttagattatg ataatgctat tcttgaagtt
      841 catggatata aaattatttc tataacaata aatactttaa gtgaatttag tgtagaaggt
      901 tatatcaatg atgactgtat aaaatctaag aactcctatg ttgaatatgt agattttaat
      961 ataataaact tagataaaat acctggaaaa ctcattaaat acaatgataa ggtatacgca
     1021 ggtagaattg aatttgatat aaatgattat gttgtaacta tagacaagcg atatgattat
     1081 agaaaagaat taaaatcaga attaaagtct aaatctggag caataataac tcatataggc
     1141 aggattagaa gaaaagatgg tagaattttt agaacaaata atacaataaa tcttttagat
     1201 aggatatcaa cagctttgag ttttatgtgt ggaagatatg tagggttttg tttggccaaa
     1261 gggtatagaa gtggtaatga agtttataga atatggaatg aaaatcaaat atctcctttt
     1321 agatatgtac caacatggtc agatacactt tcaaattatc ataatatgga aaaatatata
     1381 agccttatgt gtaaaaagct agaagatttt tattatggtt ctgctataaa gagtgttgtt
     1441 gattggtaca tagaatctct tggaagtgct accatggaaa acaatataat atctgtacaa
     1501 attgcacttg aaactttgtc atatgtaatt ctagtggaac aaaataagat acttacagat
     1561 gaagcatttg actgtaattt ggcatctaaa aatataagac tcttacttga tacatgtaag
     1621 ataccatatg gtaaacatga attaaatata tttgataata taataaaaaa taaatttgat
     1681 gatggagtgg atttagttat ttatttaaga aatagcatag ttcatccaag tagaaagact
     1741 catagagctg tgttagaggt tgaagatatt tggaatataa tttcaatagg aacgagatat
     1801 attgaacttg ttttactctt tattttaggt tatagaggag aatattcaaa cagattagta
     1861 gaaagatgtt atggtgaagt ggaagttgta ccttggaact agatttttat atttatttta
     1921 aaatatatat tgacatatat tgccttatgc tataaaatca atatcataat aataattcag
     1981 aataacatta aatgctaaga aaagaaatag taaaagtagt taatcttaaa cagagagttt
     2041 ccgattggtg agaggagcaa tagattttac tttgaacaca tctttgagca ggactctgaa
     2101 agagtagaaa tactttatta ggagatttcc ggaatccttg cacccgttat agtgctagag
     2161 tataataatt ttattgtact taaagaggtt agtgccgtga ggcattaatg aacagaggtg
     2221 gaaccacgag agttaactcc cgtcctctag cagaagctag aggcgggagt tttttaatat
     2281 aattttttaa gcgcgcagag aattatattg caatagatac actttttaaa aaataatcaa
     2341 aatattttaa aaattaaaag acgagggaga atattatgaa aaatatatta aaaaaagtag
     2401 gaatatttac aattatgtta ggtttattag gtggagttgt aggttgttca aaaccagata
     2461 atgaaaaaga taaggatgct tcaaaggaat ctaaaaaaga agtagttgta gggtttgata
     2521 atacgtttgt accaatggga tttttagatg aaaagggaga tacagtaggc tttgacgtag
     2581 acttagctaa agaaacattt aaaagacttg gaatggaagt taagtttcag cctatagatt
     2641 ggtcaatgaa ggaaactgaa ttaaatgatt caaaaactgt agatgtatta tggaatggat
     2701 attctataac agacgaaaga aagaaaatag tatcatatac agaaccatat ttacaaaata
     2761 aacagataat agttactcta agtgactcaa aaataaattc aaaagctgat ttaaaagata
     2821 aagaagttgg aacacaacaa ggttcaacag cacttgatgc agtggaaaag gataaagatt
     2881 ttatgaatag cttaaaaggt ggagcaccag ttctttatga tacttatgat aaagcactta
     2941 gagatttaga gataggtaga actagtgcag ttgttggtga tgaagtttta attagatact
     3001 atatgggaca aaagggtgaa gacaaataca aggtattaaa agacgatttt ggattagaag
     3061 attatgttgt agcaacttct aaagaaaatc cagaactttg tgagaaaata aatgaaacat
     3121 taaaagaaat gaaaaaagat ggaacttttg acaagattta tgataagtgg tttaaataga
     3181 agataagtta gctgttaaga agggggcact aaaggcatgt tacaaggttt agagatagtt
     3241 atagcaatgt tttgtataac attgatagta tcaattccat tggggattgg agttgcattt
     3301 ttaagattat caaagaataa attagtaagt ggtattacac aatgttatat attgataatg
     3361 agaggtacac ctttactttt gcaaatgata gtcatatttt acggattacc tcttttagga
     3421 attgtatttg acagatttac agctggagta gtagctttct tcttaaatta tgcagcatat
     3481 tttgcagaaa tatttagagg tggaatacag tcaatcgata gaggacaata tgaagcttca
     3541 aaagtactag gatttgataa gtttactatg tataagagag taatctttcc acaagttttt
     3601 aaaagaatac tagcacctat ttcaaatgaa gtaatcacat tagtaaaaga tacatcttta
     3661 gtatatatat taggattaaa tgatatttta agaatatctc aaatagcaat gaacagggaa
     3721 gcaagtctgt taccattatt tgaggcagga gcgatatatc ttatatttgt agctatactt
     3781 actaagggat ttgaattact tgaaaaaaaa tattcttatt atagataaag ttacaatatt
     3841 ataggaggag gttttttata tgttgaaaat caaaaactta aataaatcat ttaaaaaaaa
     3901 tagagtactt aaagatattt cttttgagtt agaagaaggt cagattggag ttttacttgg
     3961 taagtctggt gcaggaaaga ccacaatatt aaggtgtata aatggtcttg aagaatttga
     4021 tagtggagag attataatag ataatgaggt aataaaaaat aagagagata tggcaaaaat
     4081 acgaggaaaa ataggtatgg tttttcaaaa tttcaactta tttcctcata tgacagtcct
     4141 tgaaaatata attgaatccc cagttaatgt ttttaaagtt cctagaaaag aggctgaaga
     4201 gagggctaga gaacttttaa gattagttga tttagaagat aaattaaatt cttatccatt
     4261 tgaattgtca ggaggacagc aacagagagt tgctatagct aggtcatgtg cattgatgcc
     4321 aaaagtcctt tgttttgatg aacctacttc tgcacttgat attgatacta ttcaaagagt
     4381 agtcaatata atgaacagac ttaaagacaa aggtatgaca atattaataa ttacacatga
     4441 tgttgtcttc tcaaataatg ttgcagataa aattataagt ataaaagatg gtatagtaga
     4501 aaatgttcaa ataaaagaaa aaattgttta gagttttata aatataaact agggtagaat
     4561 tttataatat aaataaaggg caagtaaaat caaatatact atgccctttt gttatctcaa
     4621 aatttaatct tatattt
//
LOCUS       DAMJVK010000180         4499 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.185, whole genome shotgun sequence.
ACCESSION   DAMJVK010000180 DAMJVK010000000
VERSION     DAMJVK010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4499
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.185"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            93..392
                     /locus_tag="PC414_003698"
     CDS             93..392
                     /locus_tag="PC414_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2466045.1"
                     /translation="MKWIKWYSITCICIFIVVAFYMFIFPNKIETIDTSSAYSFVEKK
                     VPNSAVYQGYKKNPVDGTTTIYYSYDNSTHIVRLSHPEDYSRKINWDKVSNIRFD"
     gene            716..2371
                     /gene="ilvD"
                     /locus_tag="PC414_003699"
     CDS             716..2371
                     /gene="ilvD"
                     /locus_tag="PC414_003699"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HDF2466046.1"
                     /translation="MRSDIKKGIEGAPKRALMYGMGLTKEEIERPLIGIVNAQNEVIP
                     GHLHLDEIAEAAKNGVRMSGGLPLEFPAIGVCDGIAMGHVGMNYSLASRELIADSIEA
                     MAMAHGFDALVLIPNCDKIVPGMLMAAARLNIPSIVVSGGPMLPGKKNGKVYDFNSAM
                     EGVGACKDGTVSEEELEELAMNSCPGCGSCSGLFTANSMNCLTEALGMGIPYNGTAAS
                     HSGERKRIAKYAGMYVMELLKNDIKPRDILTIDAFKNAIAVDMAMAGSTNTVLHLPAI
                     AYESGIELNLDFFDEISEKTPCLTKLSPSGKHHIEDLHMAGGIPAIMNELSKINGINL
                     DCKTVTGKTIGENIRNCEIENEEVIHTLKNPYSNQGGLAILKGNLALNGAVVKKSAVA
                     EEMLVHEGPARVFNSEEEAVNAIFGKKINKGDVIVIRYEGPKGGPGMKEMLSPTSAVA
                     GMGLDKHVALLTDGRFSGATRGASIGHISPEAMEGGLIGLVEEGDIISINIPDKKLEL
                     KVDEVEIENRKLKFKPLEPKIKHGYLSRYAKLVTSANTGAVLK"
     gene            2715..3344
                     /locus_tag="PC414_003700"
     CDS             2715..3344
                     /locus_tag="PC414_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2466047.1"
                     /translation="MNREEKSKNSKEKIIQSAFSLFSSKGYDSTSTQDIINLSGLSRG
                     AMYHHFKTKEDILRSVTKELYSQMNNFLEHLVADDTLTANEKIIELVVHSANDYTRRK
                     MVHCSWLEKIPFALIEEVRNLNNVVAPNIAKIIKQGVENKEFSCEYPEELAEMLVFSI
                     DILLDPVLFKREYSEVCNRLDFLLFMLKKMDIPLIDEYGIQKFKDLFKQ"
     gene            3370..4182
                     /locus_tag="PC414_003701"
     CDS             3370..4182
                     /locus_tag="PC414_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2466048.1"
                     /translation="MFYNAKSCQMMIGSNTVNYIEFGSGKKTLIILPGLGDGLFPLHG
                     KIQAIAFAFRYKQFTKDYKVYVFSRKNQITEKYSTRDMAKDQADIMKKLGIMKAEVMG
                     VSQGGMIAQYLAIDYPELVEKLVLAVTSSKQNDTIQNVICSWIDMAKKQNYNDLMIDT
                     AKKSYSERYLKKYQLFIPFLGKVGKPKDFKRFIIQATSCIEHNAFSELNKITCPTLII
                     GGANDKIVGNNASFHLVEKIKKSEIFIYEGLGHATYEEAQDFNERVLEFLNK"
BASE COUNT         1747 a          499 c          814 g         1439 t
ORIGIN      
        1 tagttaggga tataaaaatt ttatcgtatg cataaatatt ataaatatat ggaaataata
       61 atagtatatt ttggtaaagt aggaggaatt ttatgaaatg gattaaatgg tatagtatta
      121 catgcatatg tatttttata gtagtagctt tttatatgtt tatatttcct aataaaatag
      181 aaactattga tacttcatct gcatatagct ttgttgaaaa gaaagtacca aatagtgctg
      241 tgtatcaagg atacaaaaag aatccagttg atggtactac tacaatttat tacagctatg
      301 ataattcaac tcatatagtt agattatcac atccagaaga ctattcaagg aagattaatt
      361 gggataaagt ttcaaacatc agatttgatt aatctttaat atttataaaa gtctatatat
      421 ctaaggcaaa tatattatta atcttattca tagttgagca ctttctatta tagaagtgcc
      481 tcttctgatt tatatcataa aatattactc aacaatttgt aaaatatatt agtttataaa
      541 atatattgaa aagataatat tatataatac gattgtgtaa gatattgata aaaaatcgta
      601 ataaattttc attagtatat gctaatattt ttttacaatt ttcgtaatta attaatagaa
      661 aaatatatta taataatatt atgaaaattt aaaaaagtaa gaaagaggta atattatgag
      721 aagtgatata aaaaaaggaa tagaaggagc tccaaagaga gcgttaatgt atggaatggg
      781 tcttactaaa gaagaaattg aaagaccatt aataggtata gtaaatgcac aaaatgaggt
      841 gattcctggt catctgcatc ttgatgaaat agcagaagct gcaaaaaatg gagtaagaat
      901 gagtggtggt ttgccattag aatttcctgc tataggggta tgtgatggta tagctatggg
      961 gcatgtcgga atgaattatt ccctggcttc aagagaatta atagcagatt caatagaagc
     1021 tatggctatg gcacatggtt ttgatgcttt ggtacttata cctaattgtg acaagatagt
     1081 gccaggtatg ttaatggctg ctgcaagact taatatacca agtattgttg tgagtggagg
     1141 gcctatgctt ccaggtaaaa aaaatggaaa agtatatgat tttaactctg caatggaggg
     1201 tgttggagca tgtaaagatg gaacagtaag tgaagaagaa ttagaagagt tagccatgaa
     1261 ctcttgtcct ggatgtggtt catgttctgg tcttttcaca gcaaatagca tgaattgttt
     1321 aacagaagct ttaggcatgg gtataccata taatggtact gctgcgtcac attctggaga
     1381 aagaaaaagg atagcaaaat atgcaggtat gtatgttatg gagttactta agaacgacat
     1441 aaaacctaga gatattttaa caatagatgc ttttaaaaat gctatagctg tggatatggc
     1501 aatggctggt tctacaaata cagtacttca cttacctgca atagcttatg aatcaggaat
     1561 agagcttaac ttagattttt ttgatgaaat aagtgaaaaa actccttgtt taacaaaatt
     1621 aagtccaagt ggaaaacatc atattgaaga tttacatatg gcaggaggaa taccagctat
     1681 aatgaacgaa ctttcaaaga taaatggaat aaatttagat tgcaaaaccg taacaggcaa
     1741 gactataggg gaaaatataa gaaattgtga aatagaaaat gaagaagtaa tacatacatt
     1801 aaagaatcca tatagtaacc aaggtgggct tgcaatattg aaaggaaatc ttgctctaaa
     1861 tggagctgtt gtaaaaaagt cagcagttgc agaagaaatg ttagttcatg aaggaccagc
     1921 aagagttttt aattcagaag aagaagctgt aaatgctatt tttggtaaaa aaataaacaa
     1981 aggtgatgtt atagttataa gatatgaagg tccaaagggt ggtccaggaa tgaaagaaat
     2041 gctatctcct acatcagcag ttgcaggaat gggacttgat aagcatgtag cacttcttac
     2101 tgatggtcgt ttttcagggg caactagagg ggcatccata ggccatattt ctccagaagc
     2161 tatggaaggt ggtttaatcg gactagttga agaaggagat ataatttcta taaacatacc
     2221 agacaaaaaa ttagagttaa aagtagatga agttgaaata gaaaatagaa aattaaaatt
     2281 taaaccttta gaaccgaaaa taaagcatgg atacttaagt agatatgcta aactggtaac
     2341 atcagcaaat acaggagcag ttttaaaata gtagactata tttatgtaaa ttagaaaaag
     2401 ttaagaaaat attaattgga tatcttaggt gttatagcac taatgaggta tcctttttat
     2461 tttgtatgaa aaacatagca aaatcttgat tatctttgaa aacaatacat ctctattata
     2521 aggaatacga gtacagcata gagatgtatc ttttagacct tgttgaggta atacaacttt
     2581 ttaaaagttt gatgtggaat gcttttcgtt ccttctgaac tatgaagaaa ttgaaagtaa
     2641 gttaattctt gacataataa atattaaaat gtataataaa aaacaaacag acagtctgtt
     2701 tttaggaggg aaatatgaat agagaagaaa aaagtaaaaa tagtaaagaa aaaattatcc
     2761 aatcagcatt ttcactattt tcgtctaaag gatatgattc aacatctaca caagatatta
     2821 tcaatttatc tggtctatct agaggtgcaa tgtatcatca ctttaaaact aaagaagata
     2881 tactgagaag tgtcacaaaa gaactttact cacaaatgaa taatttttta gagcatcttg
     2941 ttgctgatga cacccttaca gcaaatgaaa aaataataga attggttgtt catagtgcga
     3001 atgattacac acgtagaaaa atggtacatt gtagctggtt agaaaaaatc ccattcgctt
     3061 taatagagga agttcgtaat ctcaacaatg tagttgcacc caatattgct aagataatta
     3121 aacaaggtgt tgaaaataaa gaattttctt gtgaatatcc agaggaatta gctgaaatgc
     3181 tcgtttttag tattgatatt ttacttgacc ctgtattatt taaacgtgaa tacagtgaag
     3241 tatgtaatag gttggatttt ttattattca tgttaaaaaa aatggatatt cctctaattg
     3301 atgagtatgg tattcaaaaa tttaaagatt tatttaaaca atgataatag gaaaagtgag
     3361 gaatagacta tgttttataa tgcaaaaagt tgtcaaatga tgataggaag taatacagtg
     3421 aattatattg aatttggtag tggtaagaag acactaatta ttttaccagg attaggagat
     3481 ggtttatttc ctttacatgg aaaaatacag gcgattgctt ttgcttttag atataagcaa
     3541 tttacaaaag attataaggt ttatgtattt agtagaaaaa atcaaattac agaaaaatat
     3601 tccacaaggg atatggctaa agaccaagca gatataatga aaaaacttgg aattatgaaa
     3661 gcagaagtaa tgggtgtttc tcaaggtgga atgatagctc aatatctggc aattgattat
     3721 cctgagttag ttgagaaact tgtgttggca gttacttctt caaagcaaaa tgatactatc
     3781 caaaatgtta tttgtagttg gattgatatg gcgaagaagc agaattacaa tgatttaatg
     3841 attgacacag caaagaaatc ctattctgaa agatatttaa aaaaatacca attatttatt
     3901 ccttttttag gaaaagtagg taaaccaaag gattttaaac gatttattat tcaagcgacc
     3961 tcttgcatag aacataatgc cttttccgaa ttaaataaaa taacatgtcc aaccttaatt
     4021 ataggaggtg ctaatgataa aattgttgga aataatgctt cttttcattt agtagaaaaa
     4081 attaaaaaaa gcgaaatatt tatctatgag ggattgggac acgctacata tgaagaagca
     4141 caggatttta atgaaagagt tttagagttt ttaaataagt aaacaccttt gttaaaaata
     4201 tacatatgaa gattggaaat ttaatgttaa aaatagaaac atgaaaatat gcttaactgg
     4261 tatttttact attcataacc aatttttgat acattatcta ctataaatac aaatatagct
     4321 tcaatgtgat tatatattgt tgtattggta aagcacttat acaaacagag gaattttgta
     4381 aattcatatt atacccacat ttgttaactt atgaaaatat aatcaaaatt tttatgagct
     4441 ttttatataa aaaatataat gtttgtatac aaaaatatta aagacatttt agtaagttt
//
LOCUS       DAMJVK010000181         4484 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.186, whole genome shotgun sequence.
ACCESSION   DAMJVK010000181 DAMJVK010000000
VERSION     DAMJVK010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4484
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.186"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     misc_binding    64..298
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            348..1367
                     /gene="pheS"
                     /locus_tag="PC414_003702"
     CDS             348..1367
                     /gene="pheS"
                     /locus_tag="PC414_003702"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HDF2466049.1"
                     /translation="MQEKLLALREAALAEIKEAQSIESVESLRVKYLGKKGEITAILK
                     EMGKLSAEERPVVGKVANEVRENIELSINSKKEEINAIEKERKLKEEVIDVTQPGKVL
                     KVGKKHPITQIIDEVTDIFIGMGFSIAEGPEVETVENNFDALNAPKDHPSRDMSDTFY
                     INDGVLLRTQTSPVQVRTMRSQELPIKVIAPGRCFRSDSPDATHSPMFHQIEGLVVGK
                     DVTMAEFKGTMDIFVEKLFGSDIKTKFRPHNFPFTEPSAEVDVTCFKCGGKGCPMCKY
                     EGWIEILGSGMVHPNVLRNCGIDPEVYSGFAFGVGVERLAMLKYEIDDIRLLFENDMR
                     FLNQF"
     gene            1384..3777
                     /locus_tag="PC414_003703"
     CDS             1384..3777
                     /locus_tag="PC414_003703"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HDF2466050.1"
                     /translation="MLVSLKWLRDYVDIDMDVKEFADKMTMTGTKVETIDYYGEEIEN
                     ILVGKILEIKQHPNADKLVVTKVDIGDKVVQIVTGATNISEGDYIPVAVNGSKLPGGV
                     EIKQTDFRGELSDGMMCSAAELGIDEHYIEEYKRGGIYILDHEDSYELGKDIKDVLGL
                     KDALIDFELTSNRPDCKCMMGIAREAAATIGTKVKYPEIEVKESDEEIDFKVEIDNPD
                     LCRRYVARMVTDVKIEPSPYWMQRRLTEAGVRPISNIVDITNFVMLELGQPLHAFDIN
                     QVETGRIVVRNAKDGEKLVTLDDVERTLDKDMLVITNGEKSLGLAGVMGGANSEITSN
                     TKTVLFESANFKPENIRMTAKKVGIRSEASSRNEKDLDPNLAEIAANRAAQLVEMLGA
                     GKVLKGVVDVYPNKPEPKKLVVNPQRINHLLGVDVPMEQFVGILESLEFKCNLVANDK
                     LEIDVPSFRTDMEQEADVWEEIARIYGFENIPSVQLEGNTTAGIKTSKQKFMDALKDN
                     STAVGLNEILTYSFVSPKGVDKIRVPEGNAKRNFVKLLNPLGEETSVMRTTLIPNMLD
                     VLSTNVSHKIEEVSAFECGHIFIPQDSELPKEENRMCVGMYGKDVDFFALKGTIETIL
                     VNVGFKGYEIEPQDNNTTFHPGRCAKIVYNNKYVGTLGELHPDVIENYNLGQRVYVAE
                     IDIDFVFDNSDRTKNYVPLPKYPSTSRDIALIVKDDVFVKQIEDIIKENGQGLVESYK
                     LFDVYKGSQIEAGYKSIAYSITYRSKDKTLTDEDVAKVHDKILSELSEKLNANLRSN"
     gene            3802..4392
                     /gene="zapA"
                     /locus_tag="PC414_003704"
     CDS             3802..4392
                     /gene="zapA"
                     /locus_tag="PC414_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDF2466051.1"
                     /translation="MNKVMVKIHGAEYPMVGDKSEKFMISIADFVDKEMDKITRQNPK
                     LSLSVAAILTALNISDLLFECSDENEKLIKANEELSKKVGASNEELQLEIKSLKLTIA
                     EKEAENREAETKMKELIEIIENKKQEIFELSNTTEGSRAELDAYKNKIEELSTQLEEA
                     NERATIAENLASEFQNKAYDLQLKCTGLNNDAKNVE"
BASE COUNT         1771 a          521 c          919 g         1273 t
ORIGIN      
        1 attaaaaata gtcacataat ttgaatggaa atgatataat actaaaataa acaatataat
       61 attgtaaatg caatgaaaga ggaaagtatt ttgattaaac ttagtaaaga gataaacacc
      121 taggctggga gtgttttcta agaggtcatg agaagttccc tctggagtaa cagagctgaa
      181 attttacagt aggctttgac gtcaaaaacg cgttaagttt gttagaggtg gtttgatgat
      241 ttttaaattg ttaaactact agggtggtac cgcgaaacta tagcctttcg tccctagaca
      301 gggattgagg ggcttttttt atacaaaaaa acgaaagggt gatatgtgtg caagaaaaat
      361 tacttgcttt acgtgaagca gctttggctg aaataaaaga agcacaaagc atagaaagtg
      421 tagaaagttt aagagttaag tacttaggaa aaaaaggtga gataactgcc atacttaaag
      481 aaatgggtaa attatctgct gaagaaagac cagtagttgg taaggttgcc aatgaggtaa
      541 gagaaaacat tgaacttagc ataaattcta aaaaagaaga aataaatgct attgaaaaag
      601 aaagaaaatt aaaagaggaa gtgatagatg ttactcaacc aggaaaagtt ttaaaggttg
      661 ggaagaagca tccaataact caaattatag atgaagtaac agatatattt atcggaatgg
      721 gattctctat agcagaaggg ccagaagttg agactgttga aaacaacttt gacgcattaa
      781 acgctcctaa agaccatcca tcaagagata tgagcgatac attctatatc aatgatgggg
      841 tattacttag aactcaaaca tctccagttc aagtaagaac tatgagaagt caagagttac
      901 caataaaagt aattgcacca ggtagatgtt ttaggtcaga ctcgccagat gctacacact
      961 caccaatgtt ccatcagata gaagggcttg ttgttggaaa agatgttact atggcagaat
     1021 ttaaaggaac tatggatatc ttcgttgaaa aattgtttgg ttctgatatc aaaactaagt
     1081 ttagacctca caacttccca tttacagaac caagtgcaga ggttgatgtt acttgtttca
     1141 aatgtggtgg taaaggttgc ccaatgtgta aatatgaagg ttggatagaa atattaggtt
     1201 caggtatggt tcatccaaat gtgcttagaa attgtggaat agacccagaa gtttacagtg
     1261 gatttgcatt tggagttggg gttgaaagac ttgcaatgct taaatacgaa atagatgata
     1321 ttagattatt attcgaaaat gatatgagat tcttaaatca attttaatta ggagggtatg
     1381 tagatgttag tatctttaaa atggcttaga gactatgttg atatagacat ggatgtaaaa
     1441 gagttcgctg ataaaatgac aatgacagga actaaagttg aaacaataga ttattatggt
     1501 gaagaaatag aaaatatatt ggttggaaag attttagaaa taaaacaaca tccaaatgct
     1561 gataagttgg ttgtaactaa agtagatatt ggagataaag ttgttcaaat agttacagga
     1621 gctacaaata tatcagaagg agattatatt ccagtagctg taaatggttc taagttacct
     1681 ggaggagttg aaatcaaaca gactgatttc agaggtgaat tatcagatgg tatgatgtgt
     1741 tcagcagctg aactaggtat agatgaacat tacattgagg agtataaaag aggtggtata
     1801 tatattttag accacgaaga ttcttatgaa ttaggaaaag atataaaaga tgttttagga
     1861 ttaaaagatg ctttaataga ttttgaatta acttcaaaca gacctgattg taaatgcatg
     1921 atgggtatag ctagagaagc agctgcaact ataggaacaa aagtaaaata tcctgaaatc
     1981 gaagtaaaag aaagtgacga agagatagat ttcaaagttg agatagataa tccagattta
     2041 tgtagaagat atgttgctag aatggttaca gatgtaaaaa tagaaccttc tccatattgg
     2101 atgcaaagaa gacttacaga agcaggagta agacctataa gtaacatagt cgatataaca
     2161 aacttcgtaa tgttagagct tggtcaacca cttcatgctt ttgatataaa tcaagtagag
     2221 actggaagaa tagtagtaag aaatgctaaa gatggagaga aacttgtaac attagatgat
     2281 gttgagagaa cattagacaa agatatgcta gttataacaa atggagaaaa atcacttggt
     2341 ttagctggtg taatgggtgg tgctaactca gaaataactt ctaatacgaa gactgtactt
     2401 tttgaaagtg ccaatttcaa accagaaaac ataagaatga cagctaaaaa agttggtatt
     2461 aggtcagaag catcttcaag aaatgaaaaa gacttagacc ctaatcttgc agagatagca
     2521 gcaaatagag ctgcacaact tgttgaaatg ttaggagcag gaaaagtttt aaaaggtgtt
     2581 gtagatgtat atccaaataa accagaacct aaaaaattgg tagtaaatcc tcaaagaatt
     2641 aaccacctat taggtgtaga tgtaccaatg gagcagtttg taggaatttt agaatcatta
     2701 gagtttaaat gtaatttggt agctaatgat aaattagaaa tagatgtacc aagctttaga
     2761 acagatatgg aacaagaagc tgatgtatgg gaagaaatag ctagaattta tggatttgag
     2821 aatatacctt ctgtacaatt agaaggaaat acgacagcag gaattaaaac ttcaaaacaa
     2881 aaattcatgg atgctttaaa agataattca actgctgtag gattaaatga aatattaaca
     2941 tattcatttg taagccctaa gggagtagat aagataagag taccagaagg taatgcaaag
     3001 agaaattttg ttaaattact taacccatta ggagaagaaa cttctgtaat gagaactact
     3061 ttaataccaa atatgttaga tgttttatca actaatgtat ctcataaaat agaagaagta
     3121 tctgcatttg agtgtggaca tatattcatc ccacaagatt cagaattacc taaggaagaa
     3181 aatagaatgt gtgtaggtat gtatggtaaa gatgttgact tctttgcact aaaaggaact
     3241 atagaaacta tacttgtaaa tgtcggattt aaaggttatg aaattgaacc tcaagataat
     3301 aatactacct tccaccctgg tagatgtgct aaaatagtgt ataataataa atatgtaggt
     3361 acattaggtg aattgcatcc agatgttata gaaaactaca atctaggtca aagagtttat
     3421 gtcgctgaaa tagatattga ctttgtattt gataattctg atagaacaaa gaattatgtg
     3481 ccattaccaa aatatccatc tacatcaaga gatatagctc ttattgttaa agatgatgta
     3541 tttgtaaaac aaatagaaga tataataaag gaaaatggtc aaggattagt agaaagctac
     3601 aagttatttg atgtttacaa aggttctcaa atagaagctg gatacaagtc aattgcatat
     3661 tcaataactt atagaagtaa agataaaact ctaacagatg aagatgtagc taaggtgcat
     3721 gataagatat taagtgaatt aagcgaaaaa ttaaatgcta acttaagatc aaattaacaa
     3781 ctaatgtcgg gggaggttag gatgaacaaa gtaatggtta aaatccatgg cgctgaatat
     3841 ccaatggttg gagacaaatc agagaagttt atgattagta ttgctgattt tgtagacaaa
     3901 gaaatggata aaataacacg tcaaaacccg aaattgagct tatcagttgc tgcgatatta
     3961 actgcattaa atatatcaga ccttttattt gaatgttcag atgagaatga gaagttgatt
     4021 aaagcgaatg aagaattgag taagaaggtt ggagcatcta atgaagagct tcaattagaa
     4081 atcaaatctt taaagcttac gatagcagaa aaagaagcag aaaatcgtga agctgaaact
     4141 aagatgaaag agttaattga gattatagaa aataaaaagc aagaaatatt cgaacttagt
     4201 aatacgacag aaggttctag agcagagtta gatgcttata aaaacaagat tgaggagtta
     4261 agtactcaac ttgaagaagc aaatgaaaga gctactatag ctgaaaactt agcttctgag
     4321 tttcaaaaca aagcttatga tttacagtta aaatgtacag ggttaaataa tgatgcaaaa
     4381 aatgtggaat aaactaaaag atttgctgtc tcttgttttt tgagacagct tcttttaatg
     4441 tataaatata aatgctatat aaatatagat aatgtttaaa tgta
//
LOCUS       DAMJVK010000182         4424 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.187, whole genome shotgun sequence.
ACCESSION   DAMJVK010000182 DAMJVK010000000
VERSION     DAMJVK010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4424
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.187"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            156..1631
                     /locus_tag="PC414_003705"
     CDS             156..1631
                     /locus_tag="PC414_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HDF2466052.1"
                     /translation="MDSEFANAVELSYECAPLLESLKEYSEKDIACFDVPGHVKNRGV
                     AILNKYLGESLMKMDINSSPTMDNVSAPNGIIKNAQDLLAQAYMADEAFFITNGTTQA
                     IHAMILSVIKPGEKVLLPRNIHKSVINALILCGGIPIFIQPEFDEKLGISLNITLEKV
                     RTEIEKDCNIKALFFLNPTYYGVCADLESIIELCHKNNVLVLVDEAHGAHFPFHLDLP
                     PSAISLGADMVAVSIHKTGGALTQSSALLLNRDNVSFEKVLQSINMLQSTSASYLLMA
                     SIDGARVNLVENGEKQLSKALNLSRYAKSKLNKIDGIKVLSTEILKQKGVKFIDETKL
                     CINVKELNLTGFEVYDLLYKNFSIQVELGDSYNILALVSIGTNKSDIDRLVKALSIIA
                     KIYRKESTLNEFNMVQINPIIKLNPREAFYAPKESVEINSCIDRICGESIMAYPPGIP
                     IIAPGELITEEIMEYIIFLKNSNAYLTDVQDKNLDRILVIK"
     gene            1718..2131
                     /gene="speD"
                     /locus_tag="PC414_003706"
     CDS             1718..2131
                     /gene="speD"
                     /locus_tag="PC414_003706"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HDF2466053.1"
                     /translation="MKFETLGRHILVEYYNCDENVLKDPRLIENFMNDSALNAKATIV
                     DSVFHHFNPWGVSGAVIIAESHLTIHTWPEYGYAAADFFTCGDIDPWKSFELLEQLLK
                     SERSESTEIPRGLTTKIKKYSKKDLGKITHKPEAV"
     gene            2161..3012
                     /gene="speE"
                     /locus_tag="PC414_003707"
     CDS             2161..3012
                     /gene="speE"
                     /locus_tag="PC414_003707"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HDF2466054.1"
                     /translation="MELWYTEEWTENVRFSIKVNKHLFEGKSQFQRIDVFDSDEFGKF
                     LTIDGLMMVTYKDEFIYHEMITHVPMATNLNIKKVLVIGGGDGGTVRELSRYPQIEKI
                     DMVEIDKMVVDVSKEYMDICSCKLDDKRVSLYFEDGVNFVKCAHDKSYDLIIVDSTDP
                     IGPGEGLFSTDFYKDCYRILTDDGILVNQSESPYFDFNAKEMKRANKKLKQIFPISEV
                     YQAHIPTYPSGHWLFGFASKKLNPVKNQDRNGWEKLSLKTKYYNSDIHLGSFMLPQYV
                     KEMLDEE"
     gene            3002..3880
                     /gene="speB"
                     /locus_tag="PC414_003708"
     CDS             3002..3880
                     /gene="speB"
                     /locus_tag="PC414_003708"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HDF2466055.1"
                     /translation="MKNNFYHMNTFMSMDKNYEESNLIVFGVGFDGTTSNRPGARFAS
                     SSMRKEFYGLETYSPFLDLDLEDYNICDYGDLEISVGSTEQVLKEIYQETYKIVRDSK
                     VPFMIGGEHLVTLPAFKAVHEKYNDIYVIHFDAHTDLREEYNNSKNSHATVIKRIWDI
                     VGDNKIFQFGIRSGTKEEFKFATEEKHTYMEIGGIDTFENIVNMLNGKNIYLTIDLDV
                     LDASVFPGTGTPEPGGVNYREFQEIFKIIKNSNINIVGCDIVELSPDYDTTGVSTVIA
                     CKILRELCLIISDKIK"
     gene            complement(4060..4260)
                     /locus_tag="PC414_003709"
     CDS             complement(4060..4260)
                     /locus_tag="PC414_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDF2466056.1"
                     /translation="MKNGIVKWFNNEKGFGFISVEGEDDVFVHFSAIQNDGYKTLEEG
                     EKVSFDITQGNRGPQAENVNRI"
BASE COUNT         1747 a          496 c          758 g         1423 t
ORIGIN      
        1 tgtttttgag taacttcaaa aaaacacttg taaaaatgct gagatgttaa tataataaat
       61 atcatgagtt taataaaagt aaaaataaaa aatagaaata ataaataata aatattaaaa
      121 atatttgaat atagagagga gatgaatttg aaatcatgga tagcgagttt gcaaatgcag
      181 ttgaattaag ctatgagtgt gctccattat tagagtcgtt aaaggaatac tcagagaaag
      241 atatagcttg ttttgatgtg cctggacatg taaaaaatag aggtgtagct atattaaata
      301 aatatctagg tgaaagtctc atgaaaatgg atataaattc atcaccaact atggataatg
      361 tatctgcgcc aaatgggatt ataaaaaatg cacaagattt gctagcacaa gcatatatgg
      421 ctgatgaggc attctttata acgaatggca caactcaagc aatacatgca atgattttga
      481 gtgttataaa accaggtgaa aaagtattac ttccaagaaa tatacataaa tcagtaataa
      541 atgcgttaat actttgtgga ggaataccaa tatttataca accagaattt gatgaaaaac
      601 taggtataag tttaaatatt actcttgaaa aagttagaac agagatagaa aaagattgta
      661 atataaaagc attatttttc cttaatccaa cttactatgg agtttgtgca gaccttgaga
      721 gtattataga actatgccat aaaaataatg ttttagtgtt agttgatgaa gctcatggtg
      781 cacattttcc atttcattta gatttacctc cttctgcaat aagtttggga gctgatatgg
      841 tagctgtaag tatacataaa actggaggag cattgacaca atcttcagca ctcttattga
      901 atagagataa tgtaagtttt gaaaaggttc ttcaatcaat caatatgcta caatcaacat
      961 cagcatctta tttgcttatg gcaagtatag atggtgctag agttaatcta gttgagaatg
     1021 gtgaaaagca actgtcaaaa gcattaaatc tatctaggta tgcaaaatct aaattaaaca
     1081 aaattgatgg aataaaagtt ttatctacag aaatattgaa acaaaaaggt gttaaattta
     1141 tagatgaaac aaaattgtgt ataaatgtaa aagaattaaa tttgactgga tttgaagtat
     1201 acgatttatt atataaaaat ttttcaattc aagtagaatt gggagattca tataatatac
     1261 tggcacttgt gtccattgga acaaataaat cagatataga tagattagta aaagcacttt
     1321 ctataattgc aaagatttat agaaaagaat caacattaaa tgaatttaat atggttcaaa
     1381 taaatccaat tattaaactt aatccaagag aagcttttta tgcaccaaag gaaagtgtag
     1441 aaatcaattc atgtatagat agaatttgtg gagaatcaat aatggcatat ccaccaggta
     1501 taccgattat tgctccagga gaattgataa cagaagaaat tatggagtat attattttct
     1561 taaaaaatag caatgcatat cttacagatg tacaagataa aaacttagat agaatattag
     1621 taataaaata aattacttta ttttgcaagg gcaagtattt gttaagtaga gtttatcaaa
     1681 tagaacttat taaaagtata aaggagttgg ggttgaaatg aaatttgaaa cactaggaag
     1741 acatatacta gtagaatact ataattgtga tgaaaacgta cttaaagacc cacgtcttat
     1801 agaaaatttt atgaatgatt cagcattaaa tgcaaaagcg acaattgtgg attctgtatt
     1861 ccaccatttt aatccttggg gggtttcagg agctgttata attgctgaat ctcatttgac
     1921 aatacataca tggcctgaat atggctatgc agcagcagat ttttttactt gtggtgatat
     1981 agacccatgg aaaagttttg aattattaga gcaactttta aagtctgaaa gaagcgagtc
     2041 tacagaaatt ccaagaggct taactacaaa aattaaaaaa tattcaaaaa aagatttagg
     2101 taagattact cataaaccag aagcagtata aaacatacaa gagaaagtga ggattattat
     2161 atggaacttt ggtatacaga agaatggaca gaaaatgtaa gattttctat aaaagtaaat
     2221 aaacatctat ttgaaggaaa gtctcaattt caaaggatag atgtgtttga tagtgatgaa
     2281 tttggaaaat ttctaacaat agatggatta atgatggtaa catataaaga tgaatttata
     2341 taccatgaga tgataactca tgttcctatg gctacaaatt taaacataaa gaaagtatta
     2401 gtcataggtg gaggtgatgg aggtacagta agagaattaa gccgttatcc tcaaatagaa
     2461 aagattgaca tggttgaaat agataaaatg gttgtagatg tatctaagga gtatatggat
     2521 atatgctcat gtaaattaga tgataaaaga gtgagtttat attttgaaga tggtgttaat
     2581 tttgttaagt gtgcacatga taaatcatat gacctgataa tagtagattc aactgaccca
     2641 ataggaccag gtgaagggct tttctcaact gatttttata aggattgtta tagaatactg
     2701 actgatgatg gaattttagt aaatcaaagt gaaagtccgt attttgactt taatgctaaa
     2761 gagatgaaaa gagctaataa aaaattaaaa cagatatttc caatatcaga agtttatcaa
     2821 gcacatatac caacatatcc atctggtcac tggttgtttg gatttgcttc aaaaaaactt
     2881 aatccagtaa aaaatcaaga tagaaatgga tgggagaaat taagtttaaa aactaaatat
     2941 tataatagtg atatacattt aggttcgttt atgttacctc aatacgtgaa ggagatgcta
     3001 gatgaagaat aatttctatc atatgaacac ttttatgagt atggacaaaa attatgaaga
     3061 atctaatctt atagtatttg gtgttggatt tgatggaact acttctaaca gacctggggc
     3121 tagatttgca agtagctcta tgagaaaaga attttacggt cttgagacat atagtccttt
     3181 tttagatttg gatttagagg attataatat atgtgattat ggagatttag aaattagtgt
     3241 tggaagtaca gaacaagtct taaaagaaat ctatcaagag acatataaga ttgttagaga
     3301 ttcaaaggta ccctttatga ttggaggaga gcatttagtt acattaccag cctttaaagc
     3361 agtacatgaa aagtacaatg atatatatgt aattcatttt gatgcccata ctgatttgag
     3421 ggaagaatat aataatagta aaaattctca tgcaacagta attaaaagaa tatgggatat
     3481 tgtaggtgat aataaaatat ttcaatttgg tataaggtct gggacaaaag aagaatttaa
     3541 atttgctaca gaagaaaaac acacatacat ggaaatagga ggaatagata cgtttgaaaa
     3601 tatagttaat atgctaaatg gaaagaatat ttatctaact atagatttag atgtattgga
     3661 tgcatctgtg tttccaggaa caggtacacc agaacctggt ggtgtaaatt atagagagtt
     3721 tcaagagatt tttaagatta taaaaaactc taatataaat atagttggtt gtgacattgt
     3781 agagttaagc cctgattacg atacaacagg tgtatcgaca gttatagctt gtaaaatctt
     3841 aagagagtta tgcttaataa tatctgataa aattaaatag agtattttat ataaatagta
     3901 tatattataa tataagaagt tattttaaaa aagttagttt aagtttgacg cttttctaaa
     3961 gtaacttctt atttttgtaa ttaaaaataa atataataaa caacaaaaaa gagccctcga
     4021 tagctccttt tctaaaatat atatatttaa attaaataat tatattctat ttacgttttc
     4081 agcttgaggt cctctattac cttgagttat atcaaagcta accttttcgc cttcttctaa
     4141 agttttgtat ccatcatttt gtatagctga gaaatgtacg aaaacatcat cttctccctc
     4201 tacagatatg aacccaaatc ctttttcatt gttaaaccat tttactattc cgtttttcat
     4261 tataagaaaa cctccgcatt attaaaaaat acaaactaaa aaaataagtc tgttctatta
     4321 atataacata aattttaaat aaaagcaaat aaaattttta aaaaaattaa ttacttttag
     4381 aatttaataa aaattgttaa attatacagt gagaagtatt gtaa
//
LOCUS       DAMJVK010000183         4230 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.188, whole genome shotgun sequence.
ACCESSION   DAMJVK010000183 DAMJVK010000000
VERSION     DAMJVK010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4230
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.188"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(108..923)
                     /locus_tag="PC414_003710"
     CDS             complement(108..923)
                     /locus_tag="PC414_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2466057.1"
                     /translation="MERLKIQVLVDNNTYIDRYFVGEPAVSYYIEIDGNRILFDSGYS
                     DVFISNAEKLNIDLGNLTHVVFSHGHNDHTRGIQFLENRYDLSTVELISHPNCFIPRK
                     HGEKSIGAPFSAEEIKNIFMYNPKDKPFNLSKNCVFLGEIPSINDFEKRAKIGKCKIG
                     DLWEDDYVLDDSAIVCKTDKGIFIVTGCSHSGICNIVEYAKKVCGDDRVIGILGGFHL
                     FELNNRLDSTIQYLDKEEMRMLYPCHCVSFKVKAKMNEILNINEVGVGLTISI"
     gene            complement(1220..2662)
                     /locus_tag="PC414_003711"
     CDS             complement(1220..2662)
                     /locus_tag="PC414_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDF2466058.1"
                     /translation="MSIYKCSVCGYIYDESKNDKTWDELSEDWECPVCTKGRSYFGKI
                     STVYYEEDEKIAEDIVEDESKLNTEKEGDLNYLSTYLRRDDEVEKHMDIIHEMAVTGK
                     SIIEPMRTKLPVISWDDILIMGAQLNPLPLNEHDEVNTTTIIGKKAKKPMIIENPVYI
                     SHMSFGALSKELKIALAKGAAQNKTAMCSGEGGILPEEKEASYKYIFEYVPNKYSVTE
                     ENLKNSDAIEIKIGQGTKPGMGGHLPGEKVTEEIAKVRNKPVGQDVISPSCFEEIQSK
                     EDLKKLVDELREVSEGRPIGVKISAGHIEKDMEFIAYAKPDFVTIDGRGGATGASPKL
                     LKDATSIPTIFALYRARKYIDTHGLDIDLVITGGLRISTDFAKAIAMGADAVAIASSA
                     LMAAACQQYRICGSGKCPVGVATQDEELRKRLHIENSANRVANFLNVSLEELKTFARI
                     SGHKDIHDLSVDDLYTVNSEISNYTNIQHV"
     gene            complement(2789..3175)
                     /locus_tag="PC414_003712"
     CDS             complement(2789..3175)
                     /locus_tag="PC414_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HDF2466059.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNNVTVKVSSTTHPMTKEHHIAWVYLMTEQGGQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKAEL"
     gene            complement(3212..3601)
                     /locus_tag="PC414_003713"
     CDS             complement(3212..3601)
                     /locus_tag="PC414_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDF2466060.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLAQLTEHGFIRKVSIPGYPDRFDGRIDNHYHIICEVCGEVYDLESEVLNNLQELISE
                     ETDIKITSYNISFKGICNNCKRCSQVG"
     gene            complement(3633..4169)
                     /locus_tag="PC414_003714"
     CDS             complement(3633..4169)
                     /locus_tag="PC414_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HDF2466061.1"
                     /translation="MNLKGTKTEKNLMAGFAGESEARNKYTYYASKARKEGYNQIAAI
                     FEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGFD
                     KIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPEK
                     CPVCDHPKAYFNIKAENY"
BASE COUNT         1344 a          815 c          447 g         1624 t
ORIGIN      
        1 catataaaag tatattgatt tctcttaaaa taaacgtaac aaaaaagata ttttctatag
       61 aaaatatctt tttaaccttc aacttaaatc ttatatactt tcaaatacta tatacttatg
      121 gtcagcccta ctccaacttc atttatattc aatatttcat tcatcttcgc ttttacctta
      181 aaagaaacac agtgacaagg gtacaacatt ctcatttctt ctttatccaa atattgtatt
      241 gtactatcta acctattatt caattcaaaa agatgaaaac cacctagtat accaataact
      301 ctatcatctc cacatacctt tttagcatac tctactatat tacaaattcc actatgagaa
      361 catcctgtaa caataaaaat acctttatca gttttacaaa ctatagcaga atcatctaaa
      421 acataatcat cttcccacaa atctcctatt ttacatttac caattttagc tcttttttca
      481 aaatcattta tagaaggtat ttcacctaaa aacacacaat tcttacttag attaaatggc
      541 ttatccttag gattatacat aaaaatattt ttaatttcct cagcagaaaa tggagctcct
      601 atgcttttct ctccatgttt tctaggtata aaacagttag gatgtgaaat aagttcaact
      661 gtagataaat catacctatt ttctagaaac tggatacctc ttgtatggtc attatgccca
      721 tgagaaaaaa caacatgagt taaatttcct aaatctatat ttaatttttc agcgtttgat
      781 atgaatacat ctgaataccc tgagtcaaac aaaatcctat ttccatcaat ctcaatataa
      841 tagcttactg ctggttctcc aacaaagtat ctatcaatat aagtattatt gtcaactaaa
      901 acttgtatct tcaatctttc cataataatc actccttaaa tttttattta ctttaaagta
      961 cagaatcttt agtttaatag tacgacatca cagaatattt tacaatttaa tatctttaat
     1021 tttcataaat agaaggagtc tatctcaaaa taaagagata agattctttt tcattgctaa
     1081 aatgataaat atatttggct tcataaaaaa aagagtgact tatgaactaa aaagttcatt
     1141 ttaagacact ctcttttata aaatatattt atcatttact tttatagcta tctaaaatct
     1201 aatagtttca aacttcaatc tatacatgct gtatgtttgt ataatttgat atttcagagt
     1261 taacagtata taaatcatca acacttaaat catgtatatc tttatgacca gaaattctag
     1321 caaaagtctt aagttcttct aaacttacat ttaaaaagtt tgccactcta ttagcactgt
     1381 tttctatatg taatcttttt ctcaattcct catcctgagt agctactcct acaggacact
     1441 taccagaacc acatatacga tattgttgac acgccgctgc cataagagca gaacttgcaa
     1501 tagcaactgc atcagcaccc atagcaattg ccttagcaaa atcagtagat atacgaagcc
     1561 cacctgttat aaccaaatct atatctaatc catgtgtatc tatgtatttt ctagcacgat
     1621 aaagagcaaa tattgttgga attgaagttg catctttaag caactttgga cttgctccag
     1681 tagctccacc acgaccatca atagttacaa aatcaggttt agcataagct ataaattcca
     1741 tatctttttc aatatgacct gctgaaattt taacacctat tggacgccct tctgaaactt
     1801 ctctaagttc atctacaagt ttctttaaat cttctttgga ttgtatttcc tcaaaacaag
     1861 atggacttat tacatcttga cctacaggct tatttcttac ttttgctatt tcttcagtta
     1921 ctttttcgcc aggaagatga cctcccatac caggttttgt tccttgacca atttttatct
     1981 ctatagcatc agaattttta agattttctt cagttacact atatttattt ggtacatact
     2041 caaaaatata cttgtatgaa gcttccttct cttctggtaa tatacctcct tcaccactac
     2101 acatagctgt cttattttgg gctgcaccct ttgctaaagc tatttttagt tctttagaca
     2161 aagctccaaa tgacatgtgg gaaatataaa ctggattttc tataatcata ggtttctttg
     2221 ctttttttcc tattattgtt gttgtattta cttcatcatg ctcatttaaa ggtaatggat
     2281 ttaattgagc acccataatt aaaatatcat cccaagaaat tactggtagt ttagttctca
     2341 taggttctat tatagatttt cctgtaacag ccatttcgtg aattatatcc atatgtttct
     2401 caacttcatc atctcttctt aaatatgtac ttaagtaatt taaatctccc tctttttcag
     2461 tatttaattt agattcatct tcaactatgt cttctgctat cttttcatct tcttcatagt
     2521 aaacagtact tatctttcca aaataactac gaccttttgt acatacaggg cattcccaat
     2581 cttcacttaa ttcatcccat gttttatcat tcttagattc atcataaata tatccacaaa
     2641 cactacattt ataaattgac atattatttt ctccttatat tatattgaca ttaaacaaaa
     2701 ttaaaacttg aatttatata aatttatagg aggctatttg tatgaaaata catttatagc
     2761 ctcctttttg actcaattta tttaaatatt acagttcagc cttccataat ccatgtaaat
     2821 tacagtaagc ataggcagaa atcactttat catcatcatt caaagcaaat ttaactacag
     2881 gttctccatc tactgctaga catttgcgtt gaccaccttg ttcagtcata agatacaccc
     2941 atgctatatg atgttcttta gtcattggat gagttgtgct acttacttta acagtaacat
     3001 tatttccatc cacttcaatt acaggtacat gtttttcaac tgctgcatct gttgtattag
     3061 gaacaagttc cttcataggt tttccacaac agaaaatagg tacaccacca ctttgtatca
     3121 ttcctacaag gtttccacaa gtctcacata taaaaaattt ttgttcacta cacataaact
     3181 cacctcatta attattttta gaatttgaaa actaacctac ttggctacac cttttacaat
     3241 tattacaaat ccctttaaaa cttatgttat aagatgttat ttttatgtct gtctcctcag
     3301 atattaattc ttgtaaatta ttaagaactt cggattctaa atcgtatacc tctccacata
     3361 cttcacatat gatatgatag tgattatcta ttcgaccatc aaatctatct ggataccctg
     3421 gaatactaac ctttcttata aagccatgtt ccgttagctg agctaaattt ctatacacag
     3481 ttcctaagct taaatttgga ttatcttttt ttaagttttc atataggtaa tccgcagtag
     3541 gatgaaccgg attatttaat atttcattta aaatcagttc tcgttgttta gaaaatttca
     3601 tagttaacat tcctttttaa taatgataat ttttaataat tttcagcttt tatattgaaa
     3661 tacgcttttg gatggtcaca tactgggcat ttttctggtg cttcagtacc tacatacaca
     3721 tgaccacagt tttgacattg ccaaacaact tcttcatctc ttttaaatat ttttccttca
     3781 tttaagtttt ctaaaagctt taagtatctt tcttcatgct ctttctctat ttttccaaca
     3841 gcttcaaata aataagctat cttgtcaaat ccttcttctc tagcttcttt agcaaatttt
     3901 gcatacatat cagtccattc atagttttca cctgctgctg catcctttaa attttcctct
     3961 gttgaaggca taccatcatg taaaagttta aaccataatt tagcatgttc cttttcattg
     4021 ttagcagttt cttcaaatat agcagctatt tgattataac cttcttttct tgcttttgaa
     4081 gcgtagtatg tatatttatt tcttgcttct gactctcctg caaatcctgc cattaaattc
     4141 ttttctgttt tagttccttt taagttcata attaattcct ccctttttaa taatagtaat
     4201 gattactatt tctatattta tatattatct
//
LOCUS       DAMJVK010000184         4156 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.189, whole genome shotgun sequence.
ACCESSION   DAMJVK010000184 DAMJVK010000000
VERSION     DAMJVK010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4156
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.189"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            661..1917
                     /locus_tag="PC414_003715"
     CDS             661..1917
                     /locus_tag="PC414_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HDF2466062.1"
                     /translation="MIEKFKTLIWIKMIIRIKSVYKTYGGLFFLGLIGIPVGAIIGLI
                     DTIFGTVLLKVTDIRETYPMYLIPFLAVVGVVIAYCYFKFGGKSSKGMNLIFEVGHGE
                     EEIIPLRLVPFIISGTWLTHLFGGSAGREGVAVQIGATFSHWVGKRLPIKNACSIFLV
                     TGMAAGFAGLFETPIAAILFAMEVLVAGSLEYQSLFPAFTASFTASSVSKALGLEKFS
                     FALSSKVVFDLSIFWKLIVLGIIFGMVGGAFAWCLKLSKRKIGNRLKNPMIRIAIIGV
                     CLSVLFLLFYKGRYSGLGTNLIQNSFYGGEIYSFDWLLKFILTILTLSAGFQGGEVTP
                     LFSIGASLGVLLAGFFNLPIELVAALGYASVFGSATNTFFAPVFIGAEVFGYSYLPYF
                     FVVCAISYIFNMDKSIYSLQKISTKQ"
     gene            1980..3173
                     /locus_tag="PC414_003716"
     CDS             1980..3173
                     /locus_tag="PC414_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDF2466063.1"
                     /translation="MLTSLALIFLLGMASGGIFKRIKLPNLLGMLLTGIILGPYVLNL
                     IDNSILDISSDLRKIALIIILTRAGLSLDINDLKKVGRPAVLMCFIPATFEIIGMIVL
                     APKLLGVSILEAAVMGAVVGAVSPAIIVPKMLKLMEEGYGTEKSIPQMLLAGTSIDDI
                     FVIVMFTVFTGLAQGNSISAISFLQIPVSIILGVIVGAVIGICLAVFFKKVHMRDSAK
                     GVLLLSISFLMISLETALEGIVPFSGLLAVMSIGIFLQIKYRVVARRLSIKYSKLWVG
                     AEILLFVLVGATVDISYAFKAGIGAVILIFGVLLFRMVGVFFCLIKTNLTIKERLFCM
                     IGYIPKATVQAAIGGVPLAMGMASGQLILTLAVLAILITAPLGAFGIDVTYKKLLTSV
                     KGESK"
     gene            3374..3754
                     /locus_tag="PC414_003717"
     CDS             3374..3754
                     /locus_tag="PC414_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2466064.1"
                     /translation="MCLKKLSKLELVIMKFIWNLDIKTNSYEIIDYMKEEHNLPEKVA
                     LKTLSKLSKKRFLYVQETGKCMYYTVAIKEKAYLEFISRNVQNLLKNNFIRNLLVSFH
                     EEELTEEKIKSLENWVVNWEEAYV"
BASE COUNT         1476 a          425 c          750 g         1505 t
ORIGIN      
        1 agtgaagaaa atatttaaat catgtatcat aatataagca gagtgaagaa aatatttaaa
       61 tcatgtatca taatataagc agagtgaaga aaaatattta aataatatat cataatgtgg
      121 agtgaggaaa aatatttaag ttatgtatca taatgcaaag taaagaaaat catttaaatt
      181 atatatcata aaaataatat gaaatattga acactcataa tattagaggc tttactattt
      241 taattgatag gttatattat ttataacatt aaaaatacaa tttacataaa atagacatat
      301 gaaaaataaa aaattatata ataattatgg gaatgaggtt ctcccttgga tgataatatc
      361 ctaaactgcc aatgagctga tgacttctat gatatcatag atgccaccag ctttttttgt
      421 tgtttaaatc aataaaatgg aggtgaagta cttactaaat ttttattttt taaatttgaa
      481 gaaatattta attaatagaa aaaatagtag attatatgtt aattatttta ttaagaatgg
      541 gttaagagtg tacctatatc ctatcaattt aaaaaacaat tatgatagtc attacattga
      601 ctacagttga tgtaataaaa ctttcaaaaa atcttatgat gcaaatagaa aggaattaaa
      661 atgatagaaa aatttaaaac tttaatatgg attaaaatga taataagaat taaaagcgtt
      721 tataaaacat atggtggact atttttttta ggactcatag gtataccagt tggtgcaatt
      781 ataggtctta tagacaccat atttggaacg gttcttctaa aagttacaga tattcgagaa
      841 acatatccaa tgtatctgat accattttta gcagtagtag gagttgttat tgcttattgc
      901 tattttaaat ttggaggaaa aagtagtaaa ggaatgaatc taatatttga ggtagggcat
      961 ggagaagaag aaattattcc tttaagattg gttccattta taatatcagg tacatggtta
     1021 acccatttat ttggtggtag tgcaggacga gaaggagttg ctgttcagat tggtgcaact
     1081 ttttcacatt gggtaggtaa aaggttgcct ataaaaaatg cttgtagtat ttttctagta
     1141 actggtatgg cagctggttt tgcaggatta tttgagacac caatagcagc aatactattt
     1201 gctatggagg ttttggtagc tggttcactg gagtatcaat cgctttttcc tgcgtttact
     1261 gcttctttta cagcaagttc agtatcaaaa gctttagggt tagagaaatt ttcttttgca
     1321 ctttcttcta aagttgtatt tgacttgtca atattttgga agcttattgt tttaggaatt
     1381 atttttggaa tggttggtgg agcatttgca tggtgtctga aattgtcaaa aagaaaaata
     1441 ggtaatagat taaaaaatcc tatgataaga attgctatta ttggagtatg tcttagtgta
     1501 ttattccttt tattttataa aggaagatat tcaggtttag ggacaaattt aattcaaaat
     1561 agtttctatg gtggagaaat ttattcattt gattggctgt taaagtttat acttacaata
     1621 ctgactttgt cagcaggttt tcaaggagga gaggttacac ctttgttttc cattggagct
     1681 agtcttggtg tattgttagc aggatttttt aatttgccaa ttgaattagt tgcagcgctt
     1741 ggctatgcta gtgtctttgg tagtgctaca aatacttttt ttgcacctgt atttataggg
     1801 gcagaagttt ttggatacag ctatttacct tatttctttg tagtatgcgc tatttcatat
     1861 atatttaata tggataaatc tatttattca ctacaaaaaa tttcaacaaa acaataaata
     1921 aaacaacata atgtatgatt agaagagaaa gaatattaga gaaaggatag ggtaggaata
     1981 tgttgacaag tttagctttg atttttttat taggaatggc ctcgggaggt atttttaaga
     2041 ggataaagtt acctaactta ttgggaatgt tacttacagg aattatatta ggtccatatg
     2101 ttttaaattt gattgataat tcaatactag atatttcttc tgaccttaga aagattgcat
     2161 tgattattat attaacaagg gctggattat ctttagatat aaacgatttg aagaaagtgg
     2221 gaagacctgc tgtacttatg tgctttattc cagctacttt tgaaattatc ggaatgatag
     2281 ttttagcacc aaaattacta ggcgtttcta tattggaagc agcagtcatg ggtgcagtag
     2341 taggagcagt ttctcccgca attattgtac ctaaaatgct taaattaatg gaagaaggtt
     2401 atggtacaga aaaaagtatt ccacagatgc ttttagcagg aacttctata gacgatattt
     2461 tcgtaattgt gatgtttaca gtattcacag gattggcaca aggaaatagt atatcagcta
     2521 ttagtttttt acaaattcca gtatcaatta tattaggtgt aatagttgga gcagtgattg
     2581 gaatctgttt agcagtattt tttaagaagg ttcatatgag agattctgct aaaggggtgt
     2641 tgcttttaag tatctcattt ttaatgataa gtttagagac tgctttagag ggtattgtgc
     2701 cattttctgg attattagca gttatgagta ttgggatttt ccttcagata aaatatcgtg
     2761 tagttgcaag aagattgtcc ataaagtatt caaaactatg ggttggcgct gaaattctct
     2821 tgtttgtttt ggtaggggca acagtagata taagttatgc atttaaagca ggaattggag
     2881 ctgtaatttt aatatttgga gttcttttat tccgtatggt aggagtattc ttctgcctta
     2941 ttaagacaaa tttgacaata aaggaaaggt tgttctgtat gattggctat attccaaagg
     3001 ctacagtaca ggcagcaata ggaggtgtac cacttgcaat ggggatggca tctggacaac
     3061 ttattttgac gttagcagtt ctagctatac ttataactgc acctcttgga gcttttggaa
     3121 ttgatgtaac ttataaaaaa ttattaactt ctgtaaaagg cgagagtaag taaaaataaa
     3181 gattatttta actggtaagt tatattttgg aaaaggatta tactattgaa tacaatataa
     3241 aaaaatcatt tgggagatta agaaggtaga agttaagaac agattttaag gtatgttatg
     3301 attattagaa ttgattttgt gaaattatta ttgtaatatt taattatata gataaagaag
     3361 cggagtgata actatgtgtt taaaaaaatt atcaaaatta gagttagtta ttatgaaatt
     3421 tatttggaat ttagatataa aaaccaattc atatgagatt attgattata tgaaagaaga
     3481 acataattta ccagaaaaag tagctttaaa aactttatca aaactatcaa agaaaagatt
     3541 cttatatgtt caagaaacag gcaagtgtat gtattataca gttgcaataa aagaaaaggc
     3601 atatttagag tttatatcaa gaaatgtgca aaatcttttg aagaataatt ttataaggaa
     3661 tttattagta tcatttcacg aagaggagct aacagaggaa aagataaagt cgttagaaaa
     3721 ctgggtagta aattgggaag aagcttatgt ataaatgtta ttatttttat tacaatttag
     3781 ataattttgg ttttgtaagc tctgcaacta tttttagatg gttgcagagt ttatttttat
     3841 tttttattcc cttaagcaat acttaaaagt aaacgcattt atatttacta acttcaatta
     3901 cttctaatac ttaaattact ttttaaaaat attttgatat gtcatctcta tagtaatcta
     3961 gaacttgttt ctattcttta tataaagttt ttatgaatga tatatataat attcgttcat
     4021 ttgattaaaa ataacaaaat attaaataat tctactctga tagtttgtta aaaaaataat
     4081 aaaaaatatt aataaacaaa aaaaaaatta tcttaagaga ggagaatgtt ctaaaatata
     4141 aaaaggtttc tagatt
//
LOCUS       DAMJVK010000185         4108 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.190, whole genome shotgun sequence.
ACCESSION   DAMJVK010000185 DAMJVK010000000
VERSION     DAMJVK010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4108
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.190"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            8..406
                     /locus_tag="PC414_003718"
     CDS             8..406
                     /locus_tag="PC414_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF2466065.1"
                     /translation="MIKKIEHVVITNNNIENYINFYKKFGFSSKEMSKNYKLNARNVK
                     INLNIKGYKFLPYVKNSKASRDNLCFKINTNIDEPKKYLEQNEIQIKRGIVECTGVFG
                     NMKSIYLKKTNGNLIELSNHRITESKPWKI"
     gene            394..1332
                     /locus_tag="PC414_003719"
     CDS             394..1332
                     /locus_tag="PC414_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDF2466066.1"
                     /translation="MENLILSINVVLPLFLTMSLGYVLKKLGLFDTHTLNKMNNVCFK
                     SFLPLLLFFNIYNTDLDGSLNFKLMAVAVIIIFSIFLILVFIIPKIEKDNKTRGALIQ
                     GVFRSNFVIFGIPVTISLFGDKSIGVTSLLIAVIVPMFNVLSVVILEIFRGGKVNIKH
                     MIKSVLTNPLIIASFIGLTMLILNINIPTFLHKTIGDISKIATPLSLIILGGSFSFSA
                     IKGHIKPIILGVCGKLILVPAIFLPISIFLGFRNVELASLMIMLSAPTAVSSFTMAQQ
                     MDSDSELAGQIVVFTSGFSIITVFLIIFILKQNNLI"
     gene            complement(1716..1940)
                     /gene="secG"
                     /locus_tag="PC414_003720"
     CDS             complement(1716..1940)
                     /gene="secG"
                     /locus_tag="PC414_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HDF2466067.1"
                     /translation="MSNILMGVQVVLGILLVISIMPQDSKNALPSEFGGEGSQAYFKP
                     KGKQAFLARVTKITAVLFFINALALLIVKK"
     gene            complement(2533..3825)
                     /gene="eno"
                     /locus_tag="PC414_003721"
     CDS             complement(2533..3825)
                     /gene="eno"
                     /locus_tag="PC414_003721"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDF2466068.1"
                     /translation="MSVIELVYAREVLDSRGNPTVEVEVVLEDGAMGRAIVPSGASTG
                     AFEAVELRDGDKGRYLGKGVETAVANVNEIIAPEIEGMDAFDQPAIDAIMIELDGTPN
                     KGKLGANAILGVSMAVARAAADEIGLPLFQYLGGVNAKQLPVPMMNILNGGEHADNNV
                     DVQEFMILPVGACCFKEGLRMGAEVFHSLKKVLGEKGLACGVGDEGGFAPNLGSNREA
                     LELIVEAITKAGYKPGEDVMLGLDVAATEMYNKETKKYVLAGEGKELTAAEMVALYED
                     WSNNFPIITIEDGLDEEDWDGWKLLTEKLGNKLQLVGDDLFVTNTERLEKGIENGVAN
                     SILVKVNQIGTITETLDAIEMAKRAGYTAVISHRSGETEDSTIADLAVAVNAGQIKTG
                     APSRTDRVAKYNQLLRIEEMVGEQARYCGLKSFYNLKK"
BASE COUNT         1422 a          660 c          458 g         1568 t
ORIGIN      
        1 agttgccatg ataaaaaaaa tagaacatgt ggtcattact aataataata tagaaaatta
       61 tatcaatttt tataaaaaat ttggtttttc atctaaagaa atgagcaaaa attataaact
      121 taatgctaga aatgttaaga taaatcttaa cataaaaggt tataaatttt taccttatgt
      181 taaaaattca aaagcaagta gagataactt atgttttaaa ataaacacaa atattgatga
      241 gccaaaaaaa tacttagaac aaaatgagat tcaaattaag agaggaattg tagaatgtac
      301 aggtgtcttt ggtaatatga aatctattta tttgaaaaaa actaatggaa atttaattga
      361 attatcaaat catagaatca cggagagtaa accttggaaa atttaatcct atcaataaat
      421 gttgttcttc ctttgtttct cacaatgtca cttggatatg ttctaaaaaa attaggtcta
      481 tttgatactc atactttaaa taaaatgaat aatgtttgct tcaagtcttt cttgccatta
      541 ttattatttt ttaatatata caatacagat ttagatggtt ctcttaattt taagcttatg
      601 gctgtagctg ttataattat tttttcaatc ttcttaatct tagtctttat tattcctaaa
      661 attgaaaaag ataataagac aaggggtgca ttaatacaag gtgtatttcg tagcaatttc
      721 gttatatttg gtattcctgt gacaatttca ctatttggcg ataagagtat tggagtcaca
      781 tctttattaa ttgcagttat tgtacctatg tttaatgtat tatctgttgt aattcttgaa
      841 atttttagag gtggcaaagt aaatataaaa catatgatta aaagcgtctt aacaaatcca
      901 ctcataatcg cctcttttat aggattgaca atgctaattt taaatattaa tatacctaca
      961 tttttacaca aaactattgg tgatatttct aaaatagcta ctcctttatc acttataatt
     1021 ctaggcggct cttttagttt ttctgctata aaggggcata taaaacccat tatattgggc
     1081 gtatgtggaa aacttatact tgtacctgct atatttttac caattagcat ttttcttgga
     1141 tttagaaatg tagaattagc ttcacttatg ataatgttat ctgctccaac tgcagtttct
     1201 tcatttacaa tggcacaaca gatggattct gacagtgaat tagctggaca aattgtagta
     1261 tttacttctg gattttctat aattacagta tttttaataa tatttatttt aaagcagaat
     1321 aatcttattt aatgatattt gataacacaa attggtatta taagaaacat atataaaact
     1381 gatttaatcg cgtaaaaaca tattattata ctatttttac tatataatat aactttgtgt
     1441 atattatctc attaaacatg gtgaaagtag tgttgctata tattttttag tttgaagaaa
     1501 atatataaat acaaacaata tataaataca aataataaac gccaccaaat agtcaatcta
     1561 taaaataaaa actggactaa tcatataaaa tcattagtcc agcttttatt ttacgtactt
     1621 tttatgttga tgtactttta ttaaaaacta aatttaattt ttaaatgtct agcaaatgca
     1681 ctaattaaat aatggccagt tattcaacaa tatcactact tcttaactat aagtaatgcc
     1741 aatgcattta taaaaaataa tactgctgtt atctttgtaa ctctagcaag aaatgcttgt
     1801 tttccttttg gtttaaagta agcttgactt ccttctccac caaattctga aggtaaagca
     1861 ttcttagaat cttgaggcat tatgctaatt actagtaaaa tccctagtac tacttgcaca
     1921 cccattaaaa tgttgctcat tcttgcccct cctatctgtt tattacttaa atttcattac
     1981 tctttagtgt atcacaataa tatagccatt tcaagactat tatgctataa ataacataca
     2041 aaaaaatata ttgttactat aatatattac tatattctca atataaaaat gaataaaact
     2101 tagaagagaa aactatttaa attaatacat aaattaacac ttaaaataat ctactctcat
     2161 tgtatatatt ataaatagcc accaaaataa aacaatcatt ttaaaatatt gatataactt
     2221 ttaatttcaa cataatttaa aataaataat ccacatttag caataatact tcaatgacaa
     2281 ttatgattaa atcaaaatat gtcttcactt ttatttttta ataaaaacct cctatgcaat
     2341 tagataaaaa tgagatagaa acatttattc atattttttc tgaatctatg aataaaatta
     2401 agtaataaaa tataaaaaag gataagtgaa ttgaaaaatc aattaacact tatctctttt
     2461 atatcttata agatgagctt aaatatctta tattctctct tcattaatct tttttagtaa
     2521 ccattttaat aactattttt ttaagttata gaaagatttt aatccacaat atctagcttg
     2581 ctctccaacc atttcttcta ttcttaataa ttggttgtac ttagcaactc tatcagttct
     2641 tgatggagca ccagttttta tttgaccagc atttactgca acagctaaat ctgctatagt
     2701 agaatcttca gtttctcctg atctgtgaga tataacagca gtatatcctg ctctcttagc
     2761 catttctata gcatctaaag tttcagttat tgtacctatt tgattaactt taactaatat
     2821 agagttagct actccatttt caattccttt ttctaatctc tctgtgttag taacaaataa
     2881 gtcgtcacca actaattgta atttatttcc taatttttca gttaataatt tccatccatc
     2941 ccaatcttct tcgtctaaac catcttctat agttattatt gggaagttgt ttgaccaatc
     3001 ttcatataaa gcaaccattt cagcagcagt taactctttt ccttctcctg ctaaaacgta
     3061 ttttttagtt tctttgttgt acatttctgt agcagcaacg tcaagaccta acataacgtc
     3121 ttcacctggc ttatatccag cttttgttat agcttcaact attaattcta aagcttctct
     3181 gtttgaacct aagtttggag caaatccacc ttcgtcacct acaccacaag ctaatccttt
     3241 ttctcctaaa actttcttta acgaatggaa tacttctgct cccattctta aaccttcttt
     3301 gaaacaacaa gctcctactg gtaatatcat aaattcttga acatcaacgt tgttatcagc
     3361 atgctctcca ccgtttaata tgttcatcat tggaacaggt aattgtttag catttactcc
     3421 acctaggtat tggaataatg gtaaacctat ttcatcagca gctgctctag ctactgccat
     3481 tgaaacacct aatatagcat tagctcctaa tttacctttg tttggagttc catctaattc
     3541 tatcatgatt gcatctatag ctggttggtc aaatgcatcc attccttcta tttcaggtgc
     3601 tattatttca tttacgttag caacagctgt ttctacacct tttcctaagt atcttccctt
     3661 atctccatct cttaattcaa cagcttcaaa agctcctgta gatgctcctg atggaactat
     3721 tgctcttccc attgctccat cttctaatac tacttcaact tctacagttg gatttcctct
     3781 tgagtctaat acttctctag catatactaa ttcaataact gacatttgta tatctccctt
     3841 cgattttcta ttagttaaat tttcatttac tgtaaacatc cgtctactat tttaatctac
     3901 tttctatatt atagtacata aagttgcttt tgaaaattag gtacaaattt atttgatggg
     3961 taaatttgta aaagttccta tttttatttt attttttata atattttaat ttatataata
     4021 taaaaaaact tgtctaaaat ccaaaaccaa tcttaaatac acaaatatta taactagatt
     4081 taaaacatta aacataaaat gtacaact
//
LOCUS       DAMJVK010000186         4087 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.191, whole genome shotgun sequence.
ACCESSION   DAMJVK010000186 DAMJVK010000000
VERSION     DAMJVK010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.191"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(521..1840)
                     /locus_tag="PC414_003722"
     CDS             complement(521..1840)
                     /locus_tag="PC414_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF2466069.1"
                     /translation="MLNINVFWIFLPIFSTAIEWIVLKFLLNEFSELRKNRLTLNSSI
                     VIAIMIITMLNTIEFNINIKLFICIFITYVLYKINYVVDKWKSVFISLLYWMLLIGFD
                     SIGLSIVMTLNSIKNMNDLLEDNLLKLELIMISKSLLIVLIPLLKFIRLKTKINKRDC
                     IYLSIPLIANIIGVIVIFEFIFKDKIIDSKESLIILTISTVFLLSNLSLVSIIGRIIK
                     DNSLKIEYEITKKRMNMQYKYYLNLQEYQLKIRKLYHDMNNHIICIQNVYGKNEFADK
                     YIKDINKQIKECNTIFNTQNMILDVILSDKKSICDMNNIYFLVDINFLECNFIEMPDI
                     CSIFSNIIDNAIEACIKIKDTSIQKKIKLRGTVINRLFVIKCENTKINEVVLKNNKVI
                     TSKKDSFLHGIGISSVKSSVEKYNGNVEIYSDKNKFTITIYIPLTRN"
     gene            complement(1834..1962)
                     /locus_tag="PC414_003723"
     CDS             complement(1834..1962)
                     /locus_tag="PC414_003723"
                     /inference="COORDINATES: protein motif:HMM:NF016296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2466070.1"
                     /translation="MEYIQFICKNTVVINGKDIPVSKYRMANLKKKLTYVLGDVLC"
     gene            complement(2101..2811)
                     /locus_tag="PC414_003724"
     CDS             complement(2101..2811)
                     /locus_tag="PC414_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2466071.1"
                     /translation="MYRIVICEDDITQIAFLRECILKSLEGISSQIELFEFNSGEELL
                     ETNLEGIDIFFLDIKMLQLTGMDVAKIIRETNDTSEIIFITSIVDYIQEGYKVRAYRY
                     LLKPIDFGDLNESILSCISDIIKKRENFMLIENKGIINKILINSIMYIEVRKKVLTIH
                     TKNDTYYTKNSMDKIELELEKYNFFRCHKSYLINLEYIQFICKKTVVINDEDVPVSKY
                     RMADLKKKLTYVLGDVLC"
     gene            complement(3902..>4087)
                     /locus_tag="PC414_003725"
     CDS             complement(3902..>4087)
                     /locus_tag="PC414_003725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2466072.1"
                     /translation="KDGTIVPPDTETKVTLVFTVTHTSSGKTADTGEIEVTVPARTTT
                     VSSLLGRIAVNIFNFNK"
BASE COUNT         1525 a          582 c          305 g         1675 t
ORIGIN      
        1 ttctataaat aattaaatct tctacattaa aaatcatttc ttcagaatct atcacaacta
       61 agtcctcctc aaattagtaa aatatttttc tttaatattt attactaata tcaactttac
      121 tatctaatta gttatttaat tgttgttttt atgttcctag ttataaagtt tatcaaattt
      181 acattttatt tttttatact atatagaact tttggtcaat aatgttacca tactaaggtc
      241 gcatttgcag ataaaaataa ctctattttt ctaaatttat ttaagatata tcatgcaaat
      301 aatataacta taaaacctat caacatttta aattcaaaca aatataaatc acattttttg
      361 ttctattatt ttattgagta ctaaaaagga tgttatctta ttagcatagc attcaaccat
      421 ttttatcacc tccaaatatc ctatgcctta aagatagcat cttctatttt atctttcaat
      481 actactgctc caactgggca atttagagcc ccaactagaa ttagttccta gttaatggta
      541 tataaatagt aattgtaaac ttgtttttat ctgagtatat ttccacattt ccattatatt
      601 tttctactga actttttaca ctacttattc ctattccatg taaaaaggaa tctttcttac
      661 ttgtaataac cttattattt tttaatacaa cttcatttat ctttgtattt tcacatttta
      721 ttacaaataa cctatttata acagttcctc ttaatttaat ctttttttgt atacttgtgt
      781 ccttaatctt aatacaagct tctattgcat tatctattat atttgaaaat atactacata
      841 tatctggcat ctctataaaa ttacattcta aaaaattaat atctactaga aaatatatat
      901 tattcatatc acaaatagat tttttatcac ttaaaataac atctagaatc atattctgag
      961 tattaaatat agtattacat tcttttattt gcttatttat atctttaata tacttatctg
     1021 caaactcatt tttcccataa acattctgaa tacaaatgat atgattattc atatcatgat
     1081 atagttttct tattttcaac tgatattcct gtaaatttaa ataatattta tattgcatat
     1141 tcattctttt ttttgtaatt tcatattcta tctttaaact attatctttt attattcttc
     1201 ctattatgct tactaatgaa agatttgata gtaaaaatac agttgatatt gtaagtatta
     1261 ttaaactctc tttagaatcg ataatcttat ctttaaaaat aaattcaaat attactatta
     1321 caccaataat gtttgctatt aatggaatag ataaatatat acaatccctc ttgtttattt
     1381 ttgtttttaa tctgataaac ttaagcaatg gtattaatac tattagtaat gatttagata
     1441 tcattattaa ctctaacttc aacaaattat cctctaataa atcattcata ttttttatag
     1501 aattaagtgt cattactata cttaatccaa tactatcaaa tccaattaat aacatccaat
     1561 atagcaaact tataaataca cttttccatt tatccactac ataatttatt ttatacaata
     1621 catatgtaat aaatatacat ataaataatt taatatttat attaaactca attgtattca
     1681 acatagtaat tatcataata gcaattacta tgctactatt tagggttaat ctattctttc
     1741 ttaactcact aaattcattt aataaaaatt ttaatacaat ccattctatc gccgtagaaa
     1801 atataggtaa aaaaatccaa aatacattta tatttaacat aatacatctc ccaatacata
     1861 tgttaatttt ttctttaaat tagccattct atatttacta actggtatat ctttaccatt
     1921 aataactaca gtatttttac atataaattg aatatactcc aaattaatta aatagctctt
     1981 gtaacatttt taaaaattta ttctttctta aatcactgaa ttcatttgat aaaaatttta
     2041 atacaattca ttctatcgcc gtagaaaata taggtaaaaa aatccaaaat acatttatat
     2101 ctaacataat acatctccca atacatatgt taattttttc tttaaatcag ccattctata
     2161 tttactaact ggtacatctt catcattaat aaccacagtc tttttacata taaattgaat
     2221 atactccaaa ttaattaaat agctcttatg acatctaaaa aaattatatt tttctagctc
     2281 taattctatt ttatccatac tatttttagt ataataagta tcatttttag tatgtatagt
     2341 caaaaccttt ttcctaacct ctatatacat aatactatta attaaaattt tattgattat
     2401 acctttattt tctatcagca taaaattttc tctttttttt ataatatccg atatacaact
     2461 caaaatactt tcatttaaat ctccaaaatc tataggcttt aataaatatc tataagccct
     2521 taccttatag ccttcctgta tgtaatcaac tattgaagtt ataaatatta tttcagaagt
     2581 atcattagtt tctctaatta ttttagccac atccatgcct gttagttgaa gcattttaat
     2641 atctaaaaaa aatatatcaa taccttctaa atttgtttcc aataattctt ctcctgaatt
     2701 aaactcaaat agttcaattt gactagaaat accctctaag gactttaata tacactctct
     2761 taaaaaagct atttgtgtaa tatcatcctc acagataact attctataca taaacacccc
     2821 tccttagaca ttagatctga aaacaaataa aatatcaatt tataaataat tatttagtta
     2881 tacaactaac atggtcatca aatactatta ttcacttaaa aaatcacccc ctatttttag
     2941 tatgttaaca acaaaataag aagatatctt agatttttaa aaacaattaa tacttaatgt
     3001 agttttgtat actatttata ggtaattttt tatatcattt taaacattaa aattattgat
     3061 gtttaagaac tattatattg atagtaatat ataattaatg atatcaaaaa tttttaatta
     3121 gacttctctc ttattcaaga tttttctata taaaatctaa gaataaatag aaaactaaca
     3181 tctaaccttt tattttttat tttcgatcta tttacttgaa ttttaacata aatctatctt
     3241 ttagtatata ttaatcatgc tgaatttgtg atgacatgta tacaaaatat cgtttagtat
     3301 tttctaataa aaaatattac tgaaattaaa aatctacaat agcatttaac aagtaaaaac
     3361 atgtttacac aaatatataa tttaaggtct ttaaaataga gtatgtatta ttctaaaaat
     3421 tagttatagt tatcacataa aaatagaaaa gaattttcaa aagatttaaa gaacatatat
     3481 acttcaattg cagaagatgt aatgttaagt gaactagcat tcttcgagga aaaatagact
     3541 caaaaaatta gaaaatagaa tcaggtttta gatgatttaa gtatttattt tcaaaaaaga
     3601 aatttgattc taagttaata aagaaatatt taaaaaaatc aaaaaatttt cttaaataag
     3661 taataggttt tgtaaaaatg gaaaaatatc aaaataagac tatgattttt aaaactatag
     3721 ccttatcaat aaatttttat ttaaagaaca cacataaata tttttaaacc taatttatgt
     3781 gaagttttat gtatttttta tataagtagt attctaatct tactcatcta atataccaaa
     3841 aagactgtaa gtaagttcac ttttatgcaa acttacccac aatctctttt acacattaat
     3901 tctatttatt aaaattaaaa atatttactg ctattctacc caataaagat gatactgttg
     3961 ttgttctggc tggtactgtt acttctattt ctcctgtatc tgctgtttta ccacttgatg
     4021 tgtgtgtcac tgtaaatact aatgttactt ttgtttctgt atctggtggc actattgttc
     4081 catcttt
//
LOCUS       DAMJVK010000187         4085 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.192, whole genome shotgun sequence.
ACCESSION   DAMJVK010000187 DAMJVK010000000
VERSION     DAMJVK010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4085
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.192"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            133..1536
                     /locus_tag="PC414_003726"
     CDS             133..1536
                     /locus_tag="PC414_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF2466073.1"
                     /translation="MEKKKIGLWSLVFMNVSALYGIRWIAKSTASSFGLGLGAIPMWL
                     LFSFIYFVPGALICAELASTYQKGDGGLYDWVKEAFGEKYGFLVSWLNWTAKIFWYSS
                     FLTFLAVNVSYTIGNPALADNKMFVLILSLIIFWALSLIATKGISFTKIFTNTGALGS
                     TIPSAILIIFSFVAVFVLKKHDIASTYTIASMTPKLNMDAFVAISAIMFGLAGAETAA
                     NFITEIDKPEKNFPKAILISAGIVASLYVLGSIAITMIIPPDQITASKGVLDALSAVC
                     ASLGIGSWLVQLIAFGIAFSVLGAIVLYIASPIKMLFGSVKKGIFPDSLVEVNEHKIP
                     SKAVILQAIIVTIILLVTTLMPSVDAIYNVLVTMTALTALFPYVLLYASYIKLRKERP
                     DEIRPYTMAKSTSTCLGLAKMVLVVTVVGILLSAAPVMPTFAENVIYEIEMIGGGLLV
                     ILSGLWLWNRYEKKTAK"
     misc_binding    1627..1867
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1910..2608
                     /locus_tag="PC414_003727"
     CDS             1910..2608
                     /locus_tag="PC414_003727"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HDF2466074.1"
                     /translation="MNVIGIIGAMDEEVSILVDLMDIRETIKKASLEFYKGILEGKNV
                     VLVKCGIGKVNSALCAQILISEFKVDAIVNTGVAGALNEKLDVNDIVISTDAIQYDVD
                     TTAFGDPKGVIPRMKTSVFKADERLIDAAYKSSVEEVKTHKVLKGRVVTGDKFINSKE
                     LKEELVNDFGGYCGEMEGGAIAHVCYLNNTPFVIIRAMSDKADGSADVTYDVFVHDAA
                     NNSKDIVLNMLKSI"
     gene            2631..3632
                     /gene="trpS"
                     /locus_tag="PC414_003728"
     CDS             2631..3632
                     /gene="trpS"
                     /locus_tag="PC414_003728"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDF2466075.1"
                     /translation="MSEKKVIFSGAQPSGKMTLGNYLGAIKNWTELQDNYDCYYSIVD
                     LHAITVPQDPKVLRANTIELLAQYIACGLDPEKNTIFIQSHVKEHVELMWVLNTMTYM
                     GELSRMTQFKDKSQKSEANLNAGLFTYPVLMAADILLYQTDLVPVGDDQKQHLELARD
                     LANRFNNRFSPTFVVPEGYYPKGGARVMSLQEPTKKMSKSDSNENAFILLADDSDSIK
                     RKIKRSVTDSLGVVKYNDEQPGIKNLIDIYSNLSKKSVEEIVNMYEGKGYGIFKEDVA
                     EVVSEALRPIREKYVDLLNNKDYLEKIYSMGAEKAEKQARKTLRKVYKKVGFIERRY"
BASE COUNT         1535 a          456 c          731 g         1363 t
ORIGIN      
        1 aaggtttatt attaaaactg tttttgaata tgcaaatatt ttgaaaaata tcagtttaat
       61 aataatgaat ctattttatt aattttgaaa ttaaatttaa aataattaat agagagggga
      121 aattaaatta ggatggaaaa gaaaaaaata ggcctatgga gtttggtatt tatgaatgta
      181 tcagctttat atggtataag atggatagct aaatctacag catcaagctt tggtttagga
      241 ttaggtgcaa tacctatgtg gttattattc tcatttatat actttgtacc aggagcttta
      301 atttgtgctg aattagcttc tacatatcaa aaaggtgatg gaggtcttta cgattgggtt
      361 aaggaagcct ttggagaaaa gtatggattt ttagtttctt ggttaaactg gacagctaaa
      421 atattttggt attcttcatt tttaactttc ttagcagtaa atgtttctta tacaatagga
      481 aatccagccc ttgctgataa taagatgttt gtacttatat tatctttaat aatattctgg
      541 gcattatctc ttatagcaac taaaggaata agttttacaa aaatatttac taatacaggt
      601 gcattaggtt caacaatacc aagtgctata cttataatat tttcatttgt agcagtattt
      661 gtgttgaaaa aacatgatat agcttctaca tacacaatag cttcaatgac tccaaaactt
      721 aatatggatg catttgttgc tatatcagct ataatgtttg gtttagcagg agcagagact
      781 gctgctaact tcataacaga aatagataag cctgaaaaga acttccctaa ggcaatatta
      841 atatcagctg gaatagtagc ttcattatat gtattaggtt caattgcaat aacaatgata
      901 ataccaccag accaaattac tgcttctaaa ggtgtattag atgcactttc tgctgtttgt
      961 gcgtctttag gaattggttc ttggttggtt caattaatag cttttggtat agctttttca
     1021 gtattaggag caatagttct ttacatagca tcaccaataa aaatgttatt tggtagtgtt
     1081 aaaaaaggaa tatttccaga tagtttagta gaggtaaatg aacacaagat accttctaaa
     1141 gcagttatac ttcaagcaat aatagttact ataatactat tggtgactac attaatgcct
     1201 tctgttgatg ctatatacaa tgttcttgta actatgacag cattaactgc attattccca
     1261 tatgtattgt tatatgcttc ttatattaaa ctaagaaaag agagaccaga tgaaattaga
     1321 ccttatacta tggcaaaatc aacaagtact tgcctaggtt tagcaaaaat ggttttagtt
     1381 gtaactgtag tgggtatatt attatctgca gcaccagtta tgcctacatt tgctgaaaat
     1441 gttatatatg agatagagat gattggtgga ggtcttctag ttatattatc tggtctatgg
     1501 ttatggaata gatatgaaaa gaaaacagct aaataaaatg tattttaaaa atttcttttg
     1561 gatattataa tatatagttg acaatttata gtgtaaggaa tattataaaa taaagaaatt
     1621 aataaattaa atcctgagat aagaaatagt aaataaatat atttttgcac agagaattaa
     1681 ggatgctgag aacttaataa aaagtattta ttgaatgggt ctttgaggag taaaatgaaa
     1741 tgtaatttaa tattacagag tatttgatat cgtgtatgca cgttatagca tatattaaga
     1801 tatatatatt caattttgag tgtatataaa tttaggtggt accacggaaa atatatccgt
     1861 cctattgatt taggacggat tttttaatat agaaaattta ggaggaaaaa tgaacgtaat
     1921 aggaattata ggtgcaatgg atgaagaagt tagtatttta gttgatttaa tggatattag
     1981 agagactata aaaaaagcta gcttagaatt ttataaggga attttggaag gtaagaatgt
     2041 agtgttagta aagtgtggca taggtaaggt taactctgct ctttgtgccc agatactaat
     2101 aagcgaattt aaggttgatg ctattgttaa tacaggtgtt gcaggagctt taaacgaaaa
     2161 acttgatgtt aatgatatag taatatcaac tgatgctata cagtatgatg tagacactac
     2221 agcatttgga gaccctaagg gagttatacc aagaatgaag acttctgtat ttaaagcaga
     2281 tgaaagactt atagatgctg cttacaaatc ttctgtggaa gaagtaaaga ctcataaagt
     2341 attaaaagga agagtagtta caggagataa atttataaat tctaaagaat taaaagaaga
     2401 attagtaaat gattttggtg gatattgtgg agaaatggaa ggtggagcta tagctcatgt
     2461 atgctattta aataatactc catttgtaat aataagagca atgtctgata aggcagatgg
     2521 aagtgctgat gttacttatg atgtatttgt acatgatgct gccaataact ctaaagacat
     2581 agttttaaat atgttaaaat caatataaat atataaggag gtacaaaaaa atgagtgaaa
     2641 aaaaagttat atttagtggt gcacagccat caggcaaaat gactttagga aattatttag
     2701 gagctataaa aaactggact gaacttcaag acaattatga ttgttattat agtatagttg
     2761 atttgcatgc aataacagta cctcaagacc ctaaagtttt aagagccaat actatagaat
     2821 tacttgctca atatatagct tgtggattag acccagaaaa aaatactata tttatacaat
     2881 cacatgtcaa agagcatgtt gaacttatgt gggtattgaa tactatgact tatatgggtg
     2941 aattaagtag aatgactcaa ttcaaagata aatcacaaaa gagtgaagca aatctaaatg
     3001 caggtttatt cacatatcca gttcttatgg ctgctgacat attgttgtat caaactgacc
     3061 tagttccagt tggagatgac caaaaacaac atttagaatt agctcgtgat ttagcaaata
     3121 gatttaataa tagattttca cctacttttg tagttccaga aggatactac ccaaaaggtg
     3181 gagcaagagt gatgagtttg caagagccaa ctaaaaaaat gagtaaatcc gattcaaatg
     3241 aaaatgcatt tatactatta gcagatgatt cagattctat aaagagaaag ataaaaagaa
     3301 gtgttactga ttctttaggt gttgtaaaat acaatgatga acaaccaggt attaaaaatc
     3361 taattgatat atattctaat ttaagtaaaa agagtgtaga agagattgta aacatgtatg
     3421 aaggaaaggg ttatggcata tttaaagaag atgtggctga agtagtatca gaagctttaa
     3481 gacctataag agaaaaatat gttgaccttc ttaataataa agattattta gaaaaaatat
     3541 atagtatggg agcagaaaaa gccgaaaaac aagctagaaa aactcttaga aaagtttata
     3601 aaaaagttgg atttatagag agaagatact aattattagc tatctttact tgataaataa
     3661 attttaaagt tattaaaaag gtatatgata tgaaagagag agtctcaaag tgaacttttg
     3721 ttcatgaggc actctttttt gtatgaaata aaatatattt taatcatttc aacaatgaaa
     3781 aagaggatta tctctatttt gagacagcat cttttgataa taaaaaaaga tgagcttttg
     3841 ctcatctcaa aggggttaga ttattcttac tttggggaaa gtagaataaa ggtaaaagtt
     3901 aactgatgtc aactttgtct tttttgcata tcactactga gcataaaaaa atcagaaaac
     3961 catagccttc tgaaacaact tctaacatac attgtatttt atacccaata tgtaatttat
     4021 gagttaattt taactcttat aatgaaaatt gtcaataata tatcaaaaaa atatttttta
     4081 aattt
//
LOCUS       DAMJVK010000188         3938 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.193, whole genome shotgun sequence.
ACCESSION   DAMJVK010000188 DAMJVK010000000
VERSION     DAMJVK010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3938
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.193"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            <1..617
                     /locus_tag="PC414_003729"
     CDS             <1..617
                     /locus_tag="PC414_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HDF2466076.1"
                     /translation="LFNFKYLIIAVIFIISVTMLILISHINILIIADIKNKDICLKLN
                     IKYMFNLININRQLYPAENSKNNDKKEGMKNNIDSSILLADDLLSIYRLLKKIKIHEL
                     YSNINFGTGNIGLTSSVYVLINTLYGNLFNMIDAEKMYLNVNPDFTKDYVLGNIRIHI
                     RPRIKALFNIIIMINKIMNKNKGNKEGDSNESNRFDTESYGNNS"
     gene            571..999
                     /gene="ytfJ"
                     /locus_tag="PC414_003730"
     CDS             571..999
                     /gene="ytfJ"
                     /locus_tag="PC414_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerW family sporulation protein"
                     /protein_id="HDF2466077.1"
                     /translation="MRATGSIQSLMETTLETIKGSIDANTIIGDPIKTDTTVVVPISK
                     VTIGFGIGGGEYSKGYDDKDREIELKNEKSDTNFAGGSAGAISVQPVAFVVVESGETR
                     IMSLDSNINLVDNILSITPRVLEKIQNISQSNKNQDKNNM"
     gene            1247..1687
                     /locus_tag="PC414_003731"
     CDS             1247..1687
                     /locus_tag="PC414_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2466078.1"
                     /translation="MSDKELSILLLESMNNFHNLIKIINNERYKKDKVLTERQFFALV
                     KIRKHDKIELKNLSRDLHVSTSSLCILLNKLVEQEYVYREEDSRDRRNTFYGITKNGE
                     KILDNEILKFVSIISDKMDCLDINNKDKLFTSLEESKNIIEQLF"
     gene            1703..3154
                     /locus_tag="PC414_003732"
     CDS             1703..3154
                     /locus_tag="PC414_003732"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HDF2466079.1"
                     /translation="MNIKYSKEILKSLENAITVVALFEGEDVSTTFDKINMISRNFNS
                     KENSIDTVTLVENNNLVNVCLVGFGKRELLNREKIRIIGGNLCKKLKNILKNEAYKSS
                     DVNVLNLNLNSDEIGCFVEGILLGNYKFDKYKTKSKDFERKEVDTITIFTEKEVECQI
                     EKAKILANSTIIARNLVNEPSNVIYPKTLAVETVKLGAEFGFNVDVYEEEKIKALGMD
                     AFFAVSRGSINKPRFIVMRYFGDKESTDILGLVGKGLTYDTGGYSLKSNASMLDMKTD
                     MAGAASVIGAMCAISQSKLKKNVIAVVAACENALSGGSYKPGDIISSMAKKTIEVLNT
                     DAEGRLTLADAIYYIINNEKVTKVVDVATLTGAALILLGNVATPIVTNNDDFYCELEK
                     AATLSGERVWKMPIYDEFKDMIKGEEADLKNTGGKNAGCITAGAFIGEFVGNTPWIHM
                     DIAGTSTSSKSIGYKAKGATGEPVRTLYYLAEI"
     gene            complement(3245..3913)
                     /locus_tag="PC414_003733"
     CDS             complement(3245..3913)
                     /locus_tag="PC414_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HDF2466080.1"
                     /translation="MFKIFLFSSEQFVSLFIFGLFLYYCPKLTKNILPYSYTVEKIIC
                     TLLVIIMALEQLLLISSGNYSTLNSLPIGINYICIYLCIAILIFKQYHLFNIFFSWSL
                     VCSVGELIFSKNLGYEFPSLIYFIFIFSKCLIIYADIYMVDVRKFRVNRYALRDNLAI
                     CFIYFSFIFLLNTFTNSRYYYGFLSHSTTAIFTFIFVTSIMYIPALLFNRDTFILEKK
                     KKSK"
BASE COUNT         1647 a          357 c          671 g         1263 t
ORIGIN      
        1 aattatttaa ttttaagtat ttaataatag ctgtaatttt tattataagt gtaactatgt
       61 tgattttaat atctcatata aacatcttaa taatagctga tataaaaaac aaagatattt
      121 gtttaaagct taatataaaa tacatgttta atttaataaa tataaataga caactttatc
      181 cagctgaaaa tagtaaaaat aatgataaaa aagaggggat gaagaataat atagattcaa
      241 gtatattatt agctgatgac cttttgagca tttatagatt attaaaaaag ataaaaatac
      301 atgaacttta ttcaaatatt aattttggaa ctggaaatat tggcttaaca tcttctgtat
      361 atgtattgat aaatactttg tatggaaatt tatttaatat gattgatgca gaaaaaatgt
      421 atttaaatgt aaatccagat ttcacaaaag attatgtttt aggaaatata aggatacata
      481 taaggccaag aataaaagct ttatttaata ttataataat gattaataaa attatgaata
      541 aaaataaagg aaataaggaa ggtgatagta atgagagcaa caggttcgat acagagtctt
      601 atggaaacaa ctcttgaaac aattaaaggt tctatagatg caaatactat tataggagac
      661 cctataaaaa cagatactac agtggttgta cctatatcaa aggttacaat tggctttggt
      721 ataggaggag gagaatactc aaaaggatat gatgataaag atagggaaat tgaattaaaa
      781 aatgaaaaat cagatacgaa ttttgctgga ggtagcgcag gggctatatc tgtacaacca
      841 gtagcatttg tagtagttga aagtggagag actagaataa tgagtttaga tagtaatatc
      901 aatctagtgg ataatatatt atctataact cctagagtat tggagaaaat tcaaaatata
      961 tcacaaagta ataagaatca agataaaaac aatatgtaag aataaaatat ttttaaatat
     1021 gtatatagtt ttaagtaaag tagtggggtt aatataaatc ccactatttt gttgtgtact
     1081 ttaaacattt atatctaata agattttagt ttaaaacaat ttaaatagat aataaagttg
     1141 atatatagta taattataaa aagaaaagaa gtattatatt atagatatat aaacgcattt
     1201 gagatataag agggagttat aaatcattag ttttgaggtg aatgttatga gcgataaaga
     1261 attatctatt ttgttgttag aaagtatgaa taattttcat aatttaataa agattattaa
     1321 taacgaaaga tataaaaaag ataaggttct tacagaaaga cagttctttg ccttagtaaa
     1381 aataagaaaa catgataaaa tagagcttaa aaatctaagt agagatttgc atgtatctac
     1441 ttctagtctg tgtatactac ttaataaatt ggttgagcaa gagtatgtat atagggaaga
     1501 agatagtaga gatagacgaa atacttttta tggaataact aaaaatggag aaaagatatt
     1561 ggataatgaa atactaaagt ttgtatctat aataagtgat aaaatggatt gcctagatat
     1621 aaataataaa gataaattat ttacttcttt ggaagagtct aaaaatataa tagaacaatt
     1681 attttgaaaa ggagaattta ttatgaatat aaagtattct aaagaaatat taaaaagttt
     1741 agaaaatgct ataacagtag tagctctatt tgagggagaa gatgtttcta cgacatttga
     1801 taaaataaat atgataagca gaaattttaa ttctaaagaa aatagtatag atactgtaac
     1861 attggttgaa aataacaatt tggtaaatgt ttgtcttgtt ggttttggta agagagagtt
     1921 attgaataga gaaaaaatta gaatcatagg tgggaatttg tgtaaaaaat taaaaaatat
     1981 attgaaaaat gaagcatata agagtagtga tgttaatgta ctgaatctta atttaaatag
     2041 tgatgaaata ggttgttttg tagaagggat attacttgga aattataagt ttgataaata
     2101 taagacaaaa agtaaggatt ttgaaagaaa agaagttgat actataacta tatttactga
     2161 aaaagaagtt gagtgtcaaa tagaaaaggc taaaatatta gctaattcaa ctataatagc
     2221 tagaaatcta gttaatgaac catcaaacgt aatatatcca aaaacattag cagtagaaac
     2281 agttaaactt ggtgcagagt ttgggtttaa tgttgatgtt tatgaagaag aaaaaataaa
     2341 ggcattggga atggatgctt ttttcgctgt ttcaagaggt tcaattaata aaccaagatt
     2401 catagtaatg agatactttg gagataaaga aagtacagat atattaggtc tagtaggtaa
     2461 aggattgact tatgatactg gtggatattc tttaaaatca aatgcttcta tgctagatat
     2521 gaagacagat atggctggtg ctgcgagtgt tataggtgct atgtgtgcta tatctcaaag
     2581 taaattaaag aaaaatgtta tagcagtagt ggcagcatgt gagaatgctt tatcaggagg
     2641 ttcatacaaa cctggagata taattagttc tatggcaaag aaaactattg aagttttaaa
     2701 tacggatgca gaaggtagac ttacattagc tgatgcaata tactacatta taaacaatga
     2761 aaaagtaaca aaggtggttg atgtagctac attaactggt gctgctttaa tattgttagg
     2821 taatgtcgca actcctatag taacaaataa tgatgatttt tattgtgaat tagaaaaagc
     2881 tgccacttta tcaggggaaa gggtatggaa gatgccaatt tatgatgaat ttaaagatat
     2941 gataaaagga gaagaagcag accttaaaaa cactggtggt aaaaatgctg gatgtataac
     3001 agcaggagct tttattggtg aatttgttgg gaatactcct tggatacata tggatatagc
     3061 ggggacaagt acatcatcaa aatcaattgg atacaaagca aagggggcta caggagaacc
     3121 tgttagaaca ttgtactatt tggcagaaat ctaaaaaaca attataaaat ataatttata
     3181 aagccacgtg gatatttttg taatttaaga ttatctatgt ggctttattt tttatttaaa
     3241 taaattattt actctttttc tttttttcaa gtataaaagt atctctatta aataaaagag
     3301 caggtatgta cataatacta gtaacaaaaa taaacgtaaa aatagcagtt gtgctatgac
     3361 ttaaaaaacc ataatagtac ctagagttgg taaatgtgtt tagtaaaaat atgaatgaaa
     3421 aataaataaa acatatagct aagttatctc taagtgcata tctgttaact ctaaattttc
     3481 taacatcaac catatatata tctgcataaa ttatcaagca cttagagaag ataaagataa
     3541 aatatattaa gctaggaaat tcatagccca agtttttaga aaaaatgagt tctccaactg
     3601 aacaaactaa actccaagaa aaaaatatat taaaaagatg atactgctta aaaattagta
     3661 tggctataca cagatatata caaatataat tgattccaat tggtaaagag tttagagtac
     3721 tataattacc tgaagaaata agtaataatt gctctagagc cataattatt acaagtaatg
     3781 tacaaattat cttctcaact gtatagctat aaggtaaaat atttttggtt agttttggac
     3841 aatagtataa aaataatcca aatataaaga gacttacaaa ttgttcacta gaaaaaagaa
     3901 aaattttaaa catataacat tctctcctat aataaatt
//
LOCUS       DAMJVK010000189         3875 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.194, whole genome shotgun sequence.
ACCESSION   DAMJVK010000189 DAMJVK010000000
VERSION     DAMJVK010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3875
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.194"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(221..1471)
                     /locus_tag="PC414_003734"
     CDS             complement(221..1471)
                     /locus_tag="PC414_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HDF2466081.1"
                     /translation="MATIKPFKAIRPNKYIVDKVAALPYDVMNSKEARRIAEGNPYSF
                     LHIDKSEIDLDENIDLYDEKVYLKARENLDEFRKQEILVKDDKECIYIYKQIMDGRAQ
                     VGIVACISVDESLNGTIKKHEYTRPEKEIDRTKHIKYCDANTGTILVTYKHQRVIDDI
                     INDFMDNNEPLYDFITDDKIEHTVWKIDNDDIIDNLVDKFEKLDYLYIADGHHRTASA
                     ENVAKEMRAKNPNYTGKEEFNYFIAMIAPDENLMVLDYNRVIKDLNGLSEEEFINKIK
                     ENFELEEIEGKEKYKPDKKGTFGMYLGDKWYKMKANKNLLEIEDPVDSLDISILQDYV
                     IDAILGIDNPRVDKRIDFIGGIRGLEELEKRVNEDMKVAFAMYPTSIEDLIRVADANK
                     IMPAKSTWFEPKVRCGLFLHEINE"
     gene            complement(1503..2444)
                     /locus_tag="PC414_003735"
     CDS             complement(1503..2444)
                     /locus_tag="PC414_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HDF2466082.1"
                     /translation="MYNILVTDGIEKEAARKLRELDFNVIEQFYEKDVLGDKLKDVDV
                     LVVRSATKVTKDVIDKALEGKKLKLIVRGGVGLDNIDVKYAQANGIKVMNTPNASSIS
                     VAELTIGQLFVLARFINTANVTMRDGKWEKKKYKGTEINGKTLGLIGFGRIAKEVAKR
                     AELLGMNVIYTDIMGEAQGFNNYKFCDMEEVLENADFLSLHIPFDKNKGAVITEKEIN
                     KMKKGAYLINCARGGLVDEKDLLKALDEGKLSAAAIDVYEQEPTLNLDLVNHPRVSPT
                     PHIGASTVEAQERIGDEIVNVIQDFFLDFNNLIGVAL"
     gene            complement(2684..3760)
                     /locus_tag="PC414_003736"
     CDS             complement(2684..3760)
                     /locus_tag="PC414_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HDF2466083.1"
                     /translation="MSKKLFIPGPIDVKEEVLQKMATPMIGHRGKDASMLQKSISEKM
                     QKLFYTNNTILLSTSSGTGLMEGSIRSCTSKKAAVFSCGSFGDRWYKMAVANNVPADI
                     FKVELGEATTPEMVDKVLSTGEYDLITVTHNETSTGIRNPIEEIGEVVKKYEDVIYCV
                     DTVSSAGGIKVEVDKIGIDICITSVQKALGLPPGMSICTFSQKAIDRAKQVPFRGVYL
                     DLLAMYEYLIKKNYQYPSTPSLSHMFALDFQLDNILDEGLDNRFNRHEDMANLVRNWA
                     KKHFQIFTNENHLSNTLTVIENTQGISVSNLNSKLQERGFQIANGYGDLKEKTFRISH
                     MGDYTVEDVQELLDNIDDILGFNK"
BASE COUNT         1160 a          714 c          417 g         1584 t
ORIGIN      
        1 acatttaaaa ccttataaat atacaaatac aattatatga attttcatcc tcaattatag
       61 catagaatat gactatctat atcctaaaat tatccatttc taaaaaaaag gtaactatta
      121 ttaagaatat caacttatag aatttatatt ccacatacga ttttcaatca aaaaaacaag
      181 ggctaaaaag tgaattgtgc actttttagc cctttattaa ttattcattt atttcatgta
      241 agaatagacc acatcttact ttaggttcaa accatgttga cttagctggc attatcttat
      301 ttgcatctgc tactcttatt aaatcttcaa tactagtagg atacattgca aatgctactt
      361 tcatatcttc attaactctt ttttctaatt cttcaagacc tctaattcca ccgataaaat
      421 caattctttt atctactcta ggattatcaa ttcctaaaat agcatcaata acatagtctt
      481 gtaatataga aatgtctaat gagtcaacag ggtcttctat ttctaataaa tttttatttg
      541 ctttcatttt ataccactta tcacctaggt acattccgaa agttcctttt ttgtcaggtt
      601 tatatttttc ttttccttct atttcttcta gttcaaaatt ttcttttatt ttatttataa
      661 attcttcctc gcttaaacca tttaagtctt tgataaccct attataatcc aaaaccatta
      721 aattctcatc tggtgcaatc attgctatga agtaattaaa ttcttctttt ccagtataat
      781 ttggattttt agctctcatt tctttggcta cattttctgc tgaagctgtc ctatgatgac
      841 catctgcaat gtatagataa tctaattttt caaatttatc tactaggtta tctattatat
      901 catcattatc aatcttccat actgtatgct ctatcttgtc atctgttatg aaatcatata
      961 aaggttcatt attatccata aaatcattga ttatatcatc tatcaccctt tgatgtttgt
     1021 aagttacaag tatagttccc gtattagcat cacagtattt tatgtgttta gttctatcaa
     1081 tttccttttc tggtcgagtg tactcatgtt tttttattgt tccattcaag ctttcatcaa
     1141 ctgatataca tgctactata ccaacttgag ctctaccatc catgatttgt ttatatatat
     1201 atatacactc tttgtcatct tttactagaa tttcttgttt tcgaaactca tctaaatttt
     1261 ctcgcgcttt taaataaact ttttcatcat ataaatctat attttcatct aaatcaattt
     1321 ctgatttatc tatatgcaaa aatgaatatg gattcccttc tgcaattctt ctagcttctt
     1381 tactattcat aacatcgtaa ggaagtgctg ctactttatc tactatatat ttatttggtc
     1441 taatcgcctt aaatggttta atcgttgcca ttactaattt cctcctaatt atacaaaata
     1501 ttttataatg ctacccctat tagattgttg aagtcaagaa agaaatcttg aatcacattt
     1561 actatttcgt ctcctattct ttcttgagct tctactgtag atgccccaat atgaggtgta
     1621 ggagaaactc ttggatgatt tactaagtct aaatttaatg taggttcttg ttcataaaca
     1681 tctatagcag ctgctgataa tttaccttca tctaatgctt ttagtaaatc tttttcatca
     1741 actaaaccgc ctcttgcaca gtttattaaa taagcccctt tcttcatctt atttatttct
     1801 ttttcagtta ttactgcccc cttattctta tcaaatggta tgtgtaaaga taagaaatct
     1861 gcattttcta aaacttcttc catatcacag aatttataat tgttaaagcc ttgcgcctca
     1921 cccattatgt cagtatatat aacattcata cctaataatt ctgctctctt tgcaacttct
     1981 tttgcaattc ttccaaaacc tataagtcca agtgtctttc cattaatttc tgtaccttta
     2041 tactttttct tctcccattt tccatctctc atagttacat ttgcagtatt tatgaatctt
     2101 gcaagaacaa ataattgacc tattgtaagc tctgcaacag atatactgct tgcatttgga
     2161 gtattcataa cttttatgcc attagcttga gcatatttaa catctatatt atccagacct
     2221 actccacctc ttactatcaa cttcaacttt ttaccctcta atgctttatc tattacatct
     2281 ttagtaactt tagtagctga acgtactaca agtacatcaa catctttaag tttatctcct
     2341 aaaacatctt tttcataaaa ctgctctatc acattaaaat ctaactctct caattttctc
     2401 gctgcttctt tctctattcc atcagtaact aatatattat acataacaaa ctcctccaat
     2461 ttttattcac ttaatttttc caatttttat tttttaaagt attttcatca ataaaaaact
     2521 ttacttaatt taactttctg tttatataat tttcaaaata tttttttatt aaagtcatat
     2581 caaatttttc tattgtttgt taaaaattta atatgtaatt tttataaaat attatttatt
     2641 atatattcta aatttatctt aaaacataca tatttttaca atattattta ttaaaaccta
     2701 atatatcatc aatattatct aataattctt gtacatcttc gacagtatag tcacccatat
     2761 gtgaaattct aaaagttttt tcttttaaat ctccatagcc atttgctatt tggaatcctc
     2821 gttcttgtaa ttttgaattt aaatttgata cactaatacc ttgagtattt tctataacag
     2881 taagtgtatt ggataagtga ttttcattgg taaaaatttg gaagtgtttt ttagcccagt
     2941 ttctaactaa gtttgccatg tcttcatgtc tgttgaatct gttatctagg ccttcatcta
     3001 atatattatc taattggaaa tctaaagcga acatgtgaga aagagatggt gttgaagggt
     3061 attggtagtt tttcttgatt aagtattcat acattgctaa caaatcaaga tatactcctc
     3121 taaaaggtac ttgcttagct ctatcgattg ctttttgaga aaatgtgcaa atagacattc
     3181 ctggtggtaa tcccagtgct ttttgtactg atgttataca aatatctatt ccaatcttat
     3241 caacctcgac ttttattcca ccggctgaac ttacagtatc tacacagtag attacatcct
     3301 catacttttt aacaacttct ccaatttctt ctattggatt tcttattcca gttgaagttt
     3361 cattgtgagt tacagttatt aaatcgtatt ctccggtaga aagcacctta tcaaccattt
     3421 ctggcgttgt tgcctctcct aattcaactt taaatatatc cgctgggaca ttattagcca
     3481 ctgccatttt gtaccatcta tcaccaaagg atccacagga aaatactgct gcctttttgg
     3541 atgtacagga acgaatggag ccttccatta aaccagttcc ggatgacgtg gataaaagta
     3601 tcgtgttgtt tgtatagaat agtttttgca tcttttcact tatactcttt tggagcatag
     3661 atgcatcttt tcctctatgt cctatcattg gtgtagccat cttttgcaag acttcttctt
     3721 taacatcaat aggaccagga ataaataatt tcttactcat tataattccc ccctttttag
     3781 tttgtaatat ggatagcgtt tttactgtcc ttatttttaa taactataat atcaagctga
     3841 attaactatg tcaacataaa atttattttt tatat
//
LOCUS       DAMJVK010000190         3809 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.195, whole genome shotgun sequence.
ACCESSION   DAMJVK010000190 DAMJVK010000000
VERSION     DAMJVK010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3809
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.195"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            33..701
                     /locus_tag="PC414_003737"
     CDS             33..701
                     /locus_tag="PC414_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2466084.1"
                     /translation="MENITSYFTTILCVFICLSSVFIFTQLARVFINKKKINQKIKSR
                     NGFRYDRDFIEARREEIHIKDNNNNKNKSNNKKLKGEKVFKYDNGDLYKGEFVDGKKN
                     GFGIYIFSSKEKYEGLWKDDKMHGIGKYTYRDGSIYTGEFKYGLKNGLGKLTYPNNDI
                     YKGYFLDNKKNGKGVLYKNDGNKQAGIWEDDEQCKSLDFKDLNNNKINNVYQNRLNNN
                     KLKV"
     gene            860..1366
                     /locus_tag="PC414_003738"
     CDS             860..1366
                     /locus_tag="PC414_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HDF2466085.1"
                     /translation="MKEYVVVDLETTGLDPYKGCEIIEIGITEIKNEQIVKNYSRLIK
                     PKGIISSFITELTHISNEMVENEEPLELVLPRFRKYIGDRTIIAHNAKFDLKFLNYYL
                     RMLDLEPINNYICTVELLKKCKSYKGKNKKLETACAYYNIENINAHRADSDTLATAKL
                     FLKIKDEY"
     gene            1494..2510
                     /locus_tag="PC414_003739"
     CDS             1494..2510
                     /locus_tag="PC414_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HDF2466086.1"
                     /translation="MGYYNDNDNNKIRRKKISNSKDSNKKSKHSYTPANKKNKSSKTS
                     SSKRMSEKVDSQNLIKRDRDSFTRDNVRPLNTDYNQRERRRVEYKQREKKLKSKKRKK
                     QKRIRIVRALLSIMLVALIIFSVAGAIFTVSAIKGSPEVTKKLIRSKYISSEVVSIKQ
                     MPNDLKHAIVSIEDERFYKHKGVDVISLVRSVLNNVFTDTTQGASTIDMQVSKNLLTS
                     NDKTMKRKIRDMYNAKQMNKIMTKDEILEAYLNNMYLGKNAYGAAKGAEVYFGKKVSE
                     LNLAECAMLAGMTNNPARYIDHGEAKKRQVIVLYKMHQLGYITDKEYRIAKADDTPFK
                     SEID"
     gene            2864..3568
                     /locus_tag="PC414_003740"
     CDS             2864..3568
                     /locus_tag="PC414_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HDF2466087.1"
                     /translation="MKKFVIEDAFWNLFPSAKIGVVICNDIDNSIKDEDYKKIILKGE
                     EEALKYLEDSEFSNNQVIKVWREAFKKFKTKKGARSSIEALLKRVHNGNNIGTINPLV
                     DIYNFISLKYALPCGGEDIDKFIGDIRLTRAIGNEEFIPLGTDENSFPYEGEIIYKDD
                     GGAICRCWNWREAVRTMLTEDTNNAFLCIELVDESRFKEFENALNELAKIVQEKLGGN
                     CKISILDIDNKEVSVD"
BASE COUNT         1632 a          324 c          660 g         1193 t
ORIGIN      
        1 ttttaaataa attttttaaa gggtgatttt ttatggaaaa tataactagt tattttacaa
       61 caatactatg tgtatttata tgcttatcat ctgtatttat atttactcaa ctagctagag
      121 tatttattaa taagaagaag attaatcaaa aaattaaatc aagaaacgga tttaggtatg
      181 atagagattt tattgaagcc agaagagaag aaatacatat aaaggataac aataataata
      241 aaaataagag taacaataag aaattaaagg gagaaaaagt atttaaatac gataatggtg
      301 atttatataa aggtgagttt gttgatggaa agaaaaatgg atttgggata tatatttttt
      361 caagtaagga aaagtatgaa ggattatgga aagatgacaa gatgcatgga ataggaaaat
      421 atacttatag agatggttca atatatacag gtgaatttaa atacggatta aaaaatggtt
      481 tgggaaaatt aacatatccc aataatgaca tttataaggg atatttttta gataataaga
      541 aaaatggtaa gggtgtatta tataaaaatg atggaaataa acaagcagga atatgggaag
      601 atgatgaaca atgtaaaagt ttagatttta aagatttaaa taataataaa ataaataatg
      661 tttatcaaaa tagattaaat aacaataaat taaaagttta attattctaa taaaaaaatc
      721 tttatttatg atggaatata ggatatatct atgttgtaat ttgtaaaatc ttcctatatc
      781 atataaaatt aagtattgat tagtctatta cataaatata gaatataaat atatccattt
      841 aataataagg agaagagata tgaaagagta tgtagttgtg gatttagaaa ctacaggatt
      901 agatccatat aaaggttgtg aaataataga aataggaata acagaaataa aaaatgaaca
      961 aattgtaaaa aactattcaa ggttgataaa acctaagggg ataatttctt catttataac
     1021 agaattgaca catataagta atgaaatggt agaaaatgaa gaaccactag aattagtttt
     1081 acctagattt agaaaataca taggtgatag aactataatt gcacacaatg cgaaatttga
     1141 tttaaaattt ttaaattact atcttagaat gttagattta gaaccaataa acaattatat
     1201 atgtacagta gagttattaa aaaaatgtaa aagttataaa ggaaaaaaca agaaacttga
     1261 aacagcttgt gcttattaca acatagagaa tataaatgct catagagctg acagtgatac
     1321 attagcaact gcaaaactat ttttgaaaat aaaagatgaa tattaattat aaatatagag
     1381 aaaattagtg aaaatatcct ttataaaatt gacccttaag cttacaattt tgttctttga
     1441 tttattttgt agaacaatgt ataatgtacc ttatatgaag aagggagact aaaatgggtt
     1501 attacaatga taatgataat aataaaataa gacgtaaaaa aattagtaac agcaaagata
     1561 gtaataaaaa atctaaacat tcttatactc cggcaaataa aaaaaataaa agttctaaga
     1621 caagttctag taaaagaatg agtgaaaaag tagattcaca gaatcttata aaaagagata
     1681 gagatagttt tacaagagat aatgtaagac ctttaaatac agattacaat caaagagaaa
     1741 gaagaagagt cgagtataaa caaagagaga aaaaattaaa aagtaaaaaa agaaaaaaac
     1801 aaaaaaggat tagaatagtt agagcattat tatctattat gttagttgca ctaattatat
     1861 tttcagtggc aggtgcaata tttacagttt cagctataaa agggtcacca gaagttacta
     1921 aaaagttaat taggtctaaa tatataagta gtgaggttgt aagtataaaa caaatgccaa
     1981 atgatttaaa acatgcaata gtttccatag aagatgagag attttataag cataaaggag
     2041 tagatgttat ttctctagtt aggtcagtat taaacaatgt gtttacagat acaacacaag
     2101 gtgcaagtac tatagatatg caagtttcaa aaaacctgct tactagtaat gataaaacta
     2161 tgaagcggaa aataagggat atgtataatg ctaagcagat gaataagatt atgactaagg
     2221 atgaaattct tgaagcatat ttaaataata tgtatctagg taaaaatgca tatggagctg
     2281 caaagggagc tgaagtctac tttggtaaaa aagttagtga attaaattta gctgagtgtg
     2341 ctatgttagc tggtatgaca aataatcctg caaggtatat tgaccatgga gaggctaaga
     2401 agagacaagt gattgtactg tataagatgc atcagcttgg ttatataact gataaggagt
     2461 atagaatagc aaaagctgat gacactccat ttaaatcaga aattgattaa aattgtaaga
     2521 atatagtatt ttcaatagtt taaacaaaca aaaatgtaat aaaaaaggtg atttgccacc
     2581 catttgccac cataattata atctaatggc aaattgtttt aatgtacaaa ctagataata
     2641 gaatattgtc ttaaatgcta tattatgcta aaatttaatt gtattttgta aattaaaaaa
     2701 cttataattt agttttctag ggttccgcat tttataatgg tcaggtccaa gagaaaacgc
     2761 gcaattgttg tgtacacgga aggataaaag cctgggagat atttaagaat atttctatag
     2821 gcttttttat ttttacatat tttaaatttg aggaggtttt ctaatgaaaa aatttgttat
     2881 tgaggatgca ttttggaatc tatttcctag tgcaaaaatt ggagttgtta tttgtaatga
     2941 tatagataac tctataaaag atgaagacta taagaaaata attttgaagg gagaagaaga
     3001 agcattaaaa tacttggagg actcagaatt tagcaacaac caagtgatta aagtatggag
     3061 agaagcattt aaaaaattta aaacgaaaaa aggagcaagg tcttctattg aagcattatt
     3121 aaaaagagtt cataatggaa ataatatagg aactattaac cctttagtgg atatctacaa
     3181 ttttatctca ttaaaatatg cattaccttg tggaggggag gatatagata aatttatagg
     3241 agatattagg ctaactagag caattggtaa tgaggagttt atcccactag ggacagatga
     3301 aaattcattt ccatatgaag gagaaataat ttataaagat gatggtggag caatttgtag
     3361 atgttggaat tggcgtgaag cagtgagaac aatgcttact gaagatacta acaatgcctt
     3421 tttatgcatt gaattagtag atgaaagtag atttaaagaa tttgaaaatg ctttaaatga
     3481 attagctaaa atagttcaag aaaaattggg tgggaattgt aaaatttcaa ttttagatat
     3541 tgataataaa gaggtatcag tggattaata tggaggtgct gttgtaatgg actattctag
     3601 ttataaagat acttatattt agggtaagaa ggtctatttt agatataacg attatcaaag
     3661 gatataatat agaaaataaa aatgtagaat ataaaaagcc tttaaaataa gaaaaaaatg
     3721 tattttaaag gcttttatta tactcttttt tatttgcata attaacaata aaaatatatt
     3781 gttaattata atatttttat tgaaaaacg
//
LOCUS       DAMJVK010000191         3760 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.196, whole genome shotgun sequence.
ACCESSION   DAMJVK010000191 DAMJVK010000000
VERSION     DAMJVK010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3760
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.196"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            434..2245
                     /locus_tag="PC414_003741"
     CDS             434..2245
                     /locus_tag="PC414_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HDF2466088.1"
                     /translation="MKKKYDFLIYSAIWLVIIILFIFSLYHSIEHIKIISHTGTIRSE
                     TQKVVKQELNNERNDDLIKRLDNILIKLRTGNGENGFQRCDNKEFQQKLNQMDSMWES
                     MKKEIIKVRNGASGDKLYKLSEEYSVLSNQIVFISEKHSNAKLYSFATALFIYLIFST
                     ISLLIWEYYNKKRFKRIFYTDNLTKIKNQVAFENRAIEILHNASNKEYVLLNIDIDNF
                     KYINDTHGYEYGDKVLIIVATVLSKTFNIKETCARICSDNFVILAKYRDSLLEDIREM
                     LTNAIISELDMNVTQTISYCIGAYLVEIDNLGYKSINSMMDKANIAHKVSKTRGISST
                     VWYNENLLKQLQMENSIYNYMYKAIENEEFHMYLQPKFQISSLNVVSAEALVRWFSPE
                     LGFLSPDEFIPLFEKSGFIIELDFYMLKKACSFVKKTFMKKNQYTYPIAVNFSRVTIY
                     QNSFYQRFLDTVKEYEIPFKYIEIEVTESAFNEISQPVISILEELKKLGFLISMDDFG
                     SGYSSLSLLCSLSINGLKLDKSLLKETFNREKVYSIIQCIIEMSHRIGMSVVCEGIET
                     KKDLEFLNTVKCDVGQGFYFSKPIEEKEFFNKYVTKK"
     gene            complement(2333..2914)
                     /locus_tag="PC414_003742"
     CDS             complement(2333..2914)
                     /locus_tag="PC414_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2466089.1"
                     /translation="MSYLLKINQFERSYDIGIFKNEQSIYKFIEKIPFVKKDSSNFNY
                     TNYYMEFEAIPEYYEVNFNNYLYIITRFSFIPDGTEIFFTWEEIHCWDEKVATEKTFI
                     NGETLVDSYNFPNNEVKDYIQKRESLYIETKKYYEEKGLKIERHALGSEDGEYVELTN
                     DSILYLLDIEAVEIREQSKNIEEFLYKYKKRFK"
     gene            complement(3350..3556)
                     /locus_tag="PC414_003743"
     CDS             complement(3350..3556)
                     /locus_tag="PC414_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2466090.1"
                     /translation="MNQSQEKDLEFIKNHWDLTIIEKERNVTRIIRYSLIILAIIAFF
                     LPTKIKFSIYIFISLFCWLMYLVL"
BASE COUNT         1431 a          376 c          513 g         1440 t
ORIGIN      
        1 agtaatatac caaaaaaaag gaatatattt acataatatg ataaaataaa ttcaaagagt
       61 taaattgtag gtaaatagta ttttaattat ttattaaaat atagattatg tatatgccaa
      121 atttaaaaag agacataaag acattataca aatatattat tggggtgata ttttgtaaga
      181 aaagaagtga tgcagtaaat taattgagta atttaaaaat gatttaatga taatgttgat
      241 gaatgtttaa aaagaaattt tagaaaaacg tatattgctg tttgtgttgg aggtgatgaa
      301 tttgtagaat ttatagggta taaatgatta aactatttaa gataaataca tatcactgta
      361 aaatattgat aatcattttt tataagagat tgattatttg caaattatat atattacaaa
      421 tgaggtaaca ataatgaaga aaaaatatga ttttttaatt tattctgcta tttggctagt
      481 tattattatt ctttttatat tttctctata tcatagtata gagcatatta aaattataag
      541 tcacactggt actattcgca gtgaaactca aaaggttgtt aagcaagaat tgaataatga
      601 aagaaatgat gacttgataa aacgactgga taatattctg ataaagctaa gaacaggtaa
      661 tggagaaaat ggatttcaaa gatgtgataa taaagaattt caacaaaaac taaatcaaat
      721 ggattctatg tgggaatcta tgaagaaaga aattataaaa gttagaaatg gtgcttcagg
      781 tgataaatta tataaattga gtgaagagta ttctgtgttg tctaatcaaa ttgtttttat
      841 atctgaaaaa cactctaatg caaaattgta ttcttttgct actgcactat ttatttatct
      901 tatattctca acaataagtc ttttaatttg ggaatattat aataaaaaaa ggtttaaaag
      961 aatattttat actgataatt taacaaaaat aaaaaatcag gttgcatttg aaaacagagc
     1021 gattgaaatt ttgcacaatg catctaacaa agagtatgta ctgctaaata ttgatattga
     1081 caattttaaa tatattaatg atacacatgg atatgaatat ggagataaag ttctaataat
     1141 agttgcaact gttctttcta aaacttttaa tataaaagaa acttgtgcaa gaatttgttc
     1201 agataatttt gtaatacttg caaaatatag agattctctt ttagaggata ttcgggaaat
     1261 gctaactaat gctataatct ctgaattaga tatgaatgta actcaaacta tatcttattg
     1321 tataggagct tacttagttg aaattgataa tttaggttat aaatctatca attctatgat
     1381 ggataaggca aatattgcac acaaggtaag taaaacaaga ggaatttcct ccacagtatg
     1441 gtataatgaa aatctgttaa aacagttaca aatggaaaat agtatttata attatatgta
     1501 taaagcgata gaaaatgaag aatttcatat gtatttgcaa cctaaatttc aaatatcttc
     1561 tttaaatgtt gtgagcgcag aagctcttgt aagatggttt tctccagaac ttggtttttt
     1621 atcaccagat gaatttattc cattgtttga aaaaagtgga tttattattg aattggactt
     1681 ttatatgtta aaaaaagcgt gttcatttgt aaaaaaaacg tttatgaaaa aaaatcaata
     1741 tacatatccg attgcagtta atttttcaag ggttactatt taccaaaata gtttttatca
     1801 aaggttttta gatactgtta aagaatatga aattcctttt aagtatatag agatagaggt
     1861 aactgaaagt gcttttaatg aaatatctca accagttatt tcaattttag aggaacttaa
     1921 aaagttagga tttttaattt ctatggatga ttttggatca ggatactctt cattaagtct
     1981 tttatgttct ctatctatta atggattgaa gttagataaa agcttactta aagaaacatt
     2041 taaccgggag aaagtatata gtattattca gtgtattatt gaaatgtcac atcgtatagg
     2101 catgtctgtt gtatgtgaag gtattgaaac taaaaaagac ttggaatttt taaatacagt
     2161 taaatgtgat gtaggacaag ggttttattt ttctaaaccg atagaagaaa aagaattttt
     2221 taacaaatat gtaactaaaa aataatataa ttatttttat cagtctatac attgagtcat
     2281 tttatatctc gttaaatgac tcaatgcact gttatgtatt ttaaaatata aattatttaa
     2341 aacgtttttt atacttatat agaaattctt ctatattttt tgattgttct cttatttcaa
     2401 ctgcttctat atctagtaaa taaagtatag aatcatttgt aagttcaaca tattcaccat
     2461 cttctgaacc gagtgcatgt ctctcaattt ttaatccttt ttcttcataa tatttttttg
     2521 tttctatata taatgattct cgcttttgta tgtagtcttt cacttcatta tttggaaagt
     2581 tatatgaatc cactaatgtt tcgccattta taaaagtttt ctcggtagca actttttcat
     2641 cccaacaatg gatttcttcc caagtaaaaa atatctcagt tccatctgga atgaatgaaa
     2701 acctggtaat aatatataaa taattattga aatttacttc ataatattca ggaatagctt
     2761 caaattccat atagtaattt gtataattaa agttactact atcttttttt acaaatggta
     2821 ttttctcaat aaatttatat attgattgtt catttttaaa aattcctata tcatagcttc
     2881 tttcaaattg attaattttt aataaatatg acatatattc tccttatttg ttgaaatata
     2941 atttttataa ctctactaat tttaaacatt tttaaataaa tatttgtata taccttatat
     3001 aatattatag taattagttt taaatcaata gaatagtatg tatttatcta atgcggcaat
     3061 ataatttaaa gctggtgttg tagaaaaact aagagtgaat atagcaaaga gaagcaatag
     3121 tattttaaac tttgtctctc ttattttttg aaaactaaca ttaaaacata aggtataaat
     3181 aatattatca tataaatgaa aacaaatttg aatttttctc tggtcgatgt atatagtaag
     3241 tttgttgata tgagaaaaat tattattgat aatatacatg catagaaagg aaatggtata
     3301 taatattttc ttaagtttct tttttaggca tttctattgg cattttaggc tatagtacta
     3361 aatacattaa ccaacaaaat aatgaaataa atatatatat agaaaactta attttcgtgg
     3421 gaagaaaaaa agcaataata gcaagtatta ttagagaata acgaataata cgagtaacat
     3481 ttctttcttt ttctatgata gttaaatccc agtgattttt tataaattca aggtcttttt
     3541 cttgactctg attcatttaa tcatctcctg ttaatagtat aattttaaag ctattacttt
     3601 gttttatttt aacatattta tacactaaag tgaattttat gattagattt atttaattat
     3661 ttctgaatta aaacttatta gaattctcta ttttgataat ttcaaactct attgattgta
     3721 tgataattga aactatagga tatgtaggat aacaagtttc
//
LOCUS       DAMJVK010000192         3685 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.197, whole genome shotgun sequence.
ACCESSION   DAMJVK010000192 DAMJVK010000000
VERSION     DAMJVK010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3685
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.197"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            258..1634
                     /locus_tag="PC414_003744"
     CDS             258..1634
                     /locus_tag="PC414_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HDF2466091.1"
                     /translation="MKTIVRRLILYADGMRPTQIMVSGFAAIIVIGALLLTLPIASQS
                     GESIGLLNALFTATSAVCVTGLVMVDTATYWSLFGQIVIITLIQIGGLGFMTVATMFS
                     LMARKKIQLRERLLIQESLNQADLSGLVRLTRFVLIITITIEGIGALVLSTVFIPQFG
                     LSKGIWYSVFHAISAFCNAGFDLMGSVSGPFTSLNSYVNNFTVSMTVCALIVLGGLGF
                     PVVLDIVRKRRFSKLNVHSKVVLFSTATLIFVGALFIFLIEFNQKATMADLPLKGKVL
                     SAIFQSVTARTAGFNTLDLATLRESSVFVMIILMFIGASPASTGGGIKTTTLAVLIIT
                     VRSFLSGKSDIEAFERRLAPSTIKKSLGIFVISISAVIFGTLIISITQPNFTLVQSAF
                     EVTSALATVGSSLAGTPNLNALGKIIIIIFMFMGRVGSLTLFMAILSGGRRKSQPIRY
                     AEGKIMVG"
     gene            1945..2616
                     /locus_tag="PC414_003745"
     CDS             1945..2616
                     /locus_tag="PC414_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HDF2466092.1"
                     /translation="MKQYIVIGCGRFGSSVASTMHLLGHQVMAIDKNEDSVQSISDKV
                     THSLIVDVTDEQALRSLGLGNFDVAVVAIGSDIRASIMATLIAKEMGVELIICKAKDE
                     LQAKVLYKIGADRVVFPERDMGVRVAHNLVSDNILDHIELDPEYSIVEIVTPNSWVGK
                     TLIELELRARYEITVLAIKTGKNINVTPSPDEELTAGSILVIIGQNTSITAITSGNKG
                     IIRRR"
     gene            2805..3602
                     /locus_tag="PC414_003746"
     CDS             2805..3602
                     /locus_tag="PC414_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HDF2466093.1"
                     /translation="MITNINSKDNEKLKYTRALLKSKNRNKESKFIIEGYRIVMLALE
                     CMANLDYVFINEEFENKKEHVKLLEDLDKKNTKIYKTTNKNFKELVDTENTQGIIGVV
                     SFKKKKLSESINKKDKFVLILDRIQDPGNMGTIIRTADSAGVDAIIALKGCVDIYNPK
                     VIRSTMGSIFDMNIIDASQDETVDMLKSLDFNIVSSYLNTENFYDKIDYGSKVALVIG
                     NEANGINEELVSKSDILVKIPIYGKAESLNAAISSAILMYEIKKYLI"
BASE COUNT         1398 a          409 c          683 g         1195 t
ORIGIN      
        1 taagtacaaa atagtaaaag ctatgatagt tttagaccta ttatatgtaa aaaatataga
       61 tgtttttggt gcaatttaaa ttaaaagata tatataaaaa tttaaaatat tccaaacaga
      121 aattataaaa attactatca ttgtctttat acaatattta tactataata gaataataat
      181 ttagaggaaa gctctaaaaa taaacaaatt aagggtctta acagtagtga ttttatgtta
      241 aggaggtata aagaaagttg aaaacaatag ttagaagact tattctctac gcagatggaa
      301 tgcgtcctac tcaaataatg gtatctgggt ttgctgcgat tattgtaatc ggagcattgt
      361 tgttaacact cccgatagcg tcgcagagtg gagagagtat aggcttatta aatgctctat
      421 ttacggctac ttcagcggtt tgtgttactg gtctagttat ggtagataca gcaacttatt
      481 ggagtttatt tggacagata gttataataa cattgataca aattggtggt cttggattta
      541 tgaccgtagc cacaatgttt tcacttatgg cacgaaaaaa aatacaacta agagaaagat
      601 tattaataca agagtcatta aatcaagctg atttatcggg acttgtaaga cttacaagat
      661 ttgtattaat aataaccata acaatagagg gaataggtgc actggtttta tcaactgtct
      721 ttattccaca atttggtttg tctaaaggga tatggtatag tgtatttcac gcaatatcag
      781 cgttttgtaa tgccggattt gacttgatgg gcagtgtaag tggcccattt acatcgttaa
      841 attcttatgt aaacaacttt acagtatcaa tgactgtttg cgcacttata gtgcttggag
      901 ggctaggatt ccctgttgta cttgatattg taagaaaaag aagattttca aaattaaatg
      961 tgcattctaa ggttgtattg ttttcaactg ctacacttat ttttgtgggt gcattattta
     1021 tttttcttat agaatttaat caaaaagcta ctatggcgga cttgccttta aagggaaaag
     1081 tactatcagc aatatttcaa tcagtaacag caagaactgc tggttttaat acgcttgatt
     1141 tagctacttt acgagaaagt agtgtatttg taatgataat tttgatgttt attggagcat
     1201 cacctgcatc aactggtggg ggtataaaaa caacaacact agctgtttta atcattacag
     1261 ttagaagttt tctatcagga aaatcagata tagaagcttt tgaaagaaga ttagctccat
     1321 caactataaa aaaatctctt ggtatatttg taatcagtat atcagcagtt atttttggaa
     1381 cattgattat ttcaataact cagcctaatt ttacattagt tcaatcagct tttgaggtta
     1441 catcagcact tgctacagtt ggttcaagtt tagctggaac tccaaactta aatgctttag
     1501 gtaagattat aataataatc tttatgttta tgggtagagt tggttctctt actttattta
     1561 tggctatact ttctggtggt agaagaaaga gtcaaccaat cagatatgca gaaggtaaaa
     1621 ttatggttgg ttaagttacc aaggagtagt ataaatttat aatgggttta gagtagcact
     1681 gtaaatctgt tgataaaata aatgagccta gatgtaatta tatgaagaaa taaaatcaac
     1741 taatgaacaa aattaattaa tgaaataaaa aaacagggaa ataaaataca aataaaaagg
     1801 taaatcaaaa ttaaattaat ctgtgtgtat aaaattatat attgtgtata agttttgtat
     1861 gtgtaaagta tataagtttt gtatgtgtaa acaattaatt taagagatat tatataaatt
     1921 gtgtattagg gaggaagttt cgagatgaag caatatatag tcattgggtg tgggagattt
     1981 ggaagttcag ttgcgtctac tatgcatctt ttaggacatc aagtaatggc aatagacaaa
     2041 aatgaagatt cagttcaaag tatatctgac aaggtaaccc attcacttat agtggatgtt
     2101 actgatgagc aagcgttaag gtcattaggt ttaggtaact ttgatgtagc agtagttgca
     2161 ataggttctg atataagggc atctataatg gcgactctta tagccaaaga aatgggtgta
     2221 gagttgataa tatgtaaggc aaaggatgaa ttacaagcta aagttcttta taaaattggt
     2281 gcagatagag ttgtatttcc agaaagagat atgggagtaa gagttgcaca caatttagtt
     2341 tcggataata tattagacca tattgaactt gacccagagt attcaattgt tgaaatcgta
     2401 actccaaata gttgggttgg caagacactt atagagcttg aattaagagc tagatatgag
     2461 ataactgtac ttgctataaa aacaggtaaa aatataaatg ttacaccttc tccagatgag
     2521 gaacttacag ccggaagtat cctagttata atcggtcaaa atactagtat aacagcgata
     2581 acatctggaa ataaggggat aattagaaga agataattta ctatttaata tatatttaat
     2641 tgcaatgaaa gcagatagta tcatataatt atgaatagtt atatgatact atttttttaa
     2701 tcgaaggtag tatttatttg taagattaga taaagagaag ttaaattaaa agtaaggagg
     2761 ctgtgctaat ataaaaattt ataagttatt agcatttaga taaaatgatt acaaatataa
     2821 acagtaagga taatgaaaag ttaaagtata caagagcact attaaaatca aagaatagga
     2881 ataaagagtc aaagttcata atagaaggat acagaatagt aatgcttgca cttgaatgta
     2941 tggcaaacct tgattatgta tttatcaatg aagaatttga aaataagaaa gaacatgtaa
     3001 aactattaga agatttggat aaaaaaaaca caaagatata caagactact aataaaaact
     3061 ttaaagaatt agtggataca gaaaatactc aaggaataat aggtgtagtt tcatttaaga
     3121 aaaaaaaatt aagtgaaagt ataaataaaa aagataaatt tgtattgatt ttagacagaa
     3181 tacaagaccc aggaaatatg gggactataa taaggactgc tgattctgct ggagtagatg
     3241 ccataatagc actaaaggga tgtgtcgata tatacaaccc aaaagtaatt aggtctacta
     3301 tgggttctat ttttgatatg aatataattg atgcttcaca agatgaaact gtggacatgc
     3361 ttaaatcatt ggattttaat atagtttcaa gttacttaaa tacagaaaat ttttatgaca
     3421 aaatagatta tggctcaaaa gtagcattgg tgataggaaa cgaagcaaat ggaataaatg
     3481 aagaacttgt atcaaagtct gatattttgg ttaagatacc tatatatggt aaagccgagt
     3541 cgttaaatgc tgcgataagc tctgctatac tgatgtatga aataaaaaaa tacttaattt
     3601 aatgtattgt aataaatata atgtcatgtt ataatcttga attaaatagt agagtatcaa
     3661 aaatagtcaa tatatattaa aaaaa
//
LOCUS       DAMJVK010000193         3634 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.198, whole genome shotgun sequence.
ACCESSION   DAMJVK010000193 DAMJVK010000000
VERSION     DAMJVK010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3634
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.198"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(218..1234)
                     /locus_tag="PC414_003747"
     CDS             complement(218..1234)
                     /locus_tag="PC414_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDF2466094.1"
                     /translation="MVTIKDIARNLGISYSTVSRCLNNNPNVSEKTKKKVVEEANRLG
                     FHFNVNARNLAKKETNRIGVIFSNNFNHQDTRKFFSDIMDSSINSIETNKYDFIIQPN
                     NNISGDSNVYKMVNGQMVDGLVIVSQSIKKEEYDFLKDNNFPHVFIYFKPSFIGEVDN
                     FFWDDNVYGGYIATKHLIDHGHKDIITVTSDDKSSKMHEDRTKGYLNAMEEANLKTEV
                     IKSKMDFESQVEFLEKYIDKIKKASAIFVQQDVPAISIIQELKTTYGINVPEDISIIG
                     YNNIELISYFRSHLSTIDDPREQVIKNGVDSLVNIINKKSTEHIPRKLYPRLIIRNSV
                     KRIK"
     gene            complement(1417..1779)
                     /gene="spoVAE"
                     /locus_tag="PC414_003748"
     CDS             complement(1417..1779)
                     /gene="spoVAE"
                     /locus_tag="PC414_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HDF2466095.1"
                     /translation="MLMDYVRVFIVGGIICLIAQVMMDYFKMQTPYVMVTYVTTGVVL
                     TFLGLYEPLVKFAAAGATVPIIGFGYSLATGVIKSIQSDGFLGIFLGGTTATAGGIAA
                     AIFFGYIMSVVFTPKAKP"
     gene            complement(1793..2806)
                     /gene="spoVAD"
                     /locus_tag="PC414_003749"
     CDS             complement(1793..2806)
                     /gene="spoVAD"
                     /locus_tag="PC414_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HDF2466096.1"
                     /translation="MKNKRIGKRTVKLENKPTIISTGTIVGPKEGEGPLKDYFDMIMT
                     DDLYGEKTWELAESKMVETASQQAIQKAGKKLSDVNYMLGGDLINQIVPASFAARELA
                     IPFLGIYGACSTMAEGLCIGSMLIDGGFADLVLSGTSSHYCTAERQFRFPLELGNQKP
                     MTAQWTVTGAGSVLLAPNGDGPKVKYVTVGKVIDEGIDDGNNMGPAMAPAAIDTIYSY
                     FEDTNDDPNSFDIIATGDLGTLGKQIAEDFLKEKGVDISKVYTDCGIEMFNLKEQDVH
                     CGGSGCGCSATVFAGYIYDKLRKKEFNKVMLVSTGALLSPTSTLQKQTIPCVAHAVVI
                     VNE"
     gene            complement(2973..3410)
                     /gene="spoVAC"
                     /locus_tag="PC414_003750"
     CDS             complement(2973..3410)
                     /gene="spoVAC"
                     /locus_tag="PC414_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HDF2466097.1"
                     /translation="MDKNYKKYVDQISPKPTYLKNYTLAFIVGGIICMIGQAINDLYM
                     KVGGLDKLGASSATSITLIFIGAFLTGLGVYDLIGKRAGAGSIIPITGFANSIVSPAM
                     EYKREGYVLGVGANLFKIAGPVLVYGIGSSILCGIIYYIFKMF"
BASE COUNT         1185 a          644 c          387 g         1418 t
ORIGIN      
        1 aaatacaaat aatttattaa tttatattgt taaaataacg gattttcaaa taattgaata
       61 aaattattca ttcatattat aaaaactatc tcaataaata aaggagtaca atgtacttat
      121 atacacctac tccttttaat ttttatttat attatttgtt aaaaaacttc acataatcta
      181 taatattttt aataaacgtt tgatacttaa aagcttacta ctttattctt ttaacgctat
      241 ttcgtattat taaccttgga tataattttc ttggaatgtg ttctgtactc tttttattta
      301 ttatatttac taaactatct actccatttt ttataacttg ctctcttggg tcatctattg
      361 ttgataaatg cgacctaaaa tatgatatta attcaatatt attataccct attatggaaa
      421 tatcttctgg tacatttata ccataagtag tttttaactc ttgtattata gaaatcgctg
      481 gtacatcttg ttgtacaaat atagctgaag ctttttttat tttatcaatg tatttttcta
      541 aaaattcaac ttgagattca aaatccattt tagatttaat gacttctgtc tttaaatttg
      601 cttcttccat agcattcaaa tatcccttgg ttctatcttc atgcattttt gaactcttat
      661 catctgaagt tactgttatt atatctttat gaccatggtc aattaaatgc ttggtagcta
      721 tataacctcc atacacgtta tcgtcccaaa agaaattatc tacttctcct ataaaagatg
      781 gtttaaaata tataaataca tgtggaaaat tattgtcttt taaaaaatca tactcttctt
      841 tttttattga ttgagacaca attactaatc cgtcaaccat ttgtccattt accattttat
      901 atacattact atcaccacta atgttattat taggctgtat tataaaatcg tatttattag
      961 tctcaataga atttatagaa ctatccatta tatcactaaa aaactttcta gtatcttgat
     1021 gattaaaatt atttgaaaag attaccccta tcctgttagt ttcttttttt gctaaatttc
     1081 ttgcatttac attaaaatgg aaccctagtc tatttgcctc ttctaccact ttcttttttg
     1141 ttttttcaga tacattagga ttattgttta agcacctaga aacagttgaa taactgatac
     1201 ctagattcct tgcaatatct ttaatagtta ccatctttat cccctacttc acttcatttt
     1261 attttgataa tataatacta cactatgttg taaataattg tcaacttcaa ttatgtaaac
     1321 gcttctttat ataaaaaaca ggtggaatct aaaagatatc cacctgcttt ataaaaagtt
     1381 attatttcaa aaacttatta ttcttttaat aaagatttat ggttttgctt ttggagtaaa
     1441 tactactgac ataatatatc caaagaatat agctgctgct attccaccag ctgtagcagt
     1501 tgtacctcct aaaaaaatac ctaaaaaacc atcactttga attgatttta ttactcctgt
     1561 agcaagtgaa tatccaaatc ctattatagg aactgttgca cctgctgctg caaacttaac
     1621 taatggctca taaagtccta gaaaagttaa aactacacca gttgttacat acgttaccat
     1681 aacatatgga gtttgcattt tgaaataatc catcattact tgggctatta aacatataat
     1741 tccacctaca ataaatactc ttacatagtc cattaacata ttatatcact tcctactcat
     1801 ttactattac tactgcatgt gcaacacatg gtattgtctg cttttgtagt gtacttgtcg
     1861 gtgaaagtag tgctcctgtt gatacaagca ttactttgtt aaattctttt tttctcaact
     1921 tatcatatat atatcctgca aacactgtag ctgaacaccc acatccactt ccaccacagt
     1981 ggacatcttg ttctttaagg ttaaacatct ctataccaca gtcagtataa acttttgata
     2041 tatctacacc tttttctttt agaaagtctt ctgctatctg ttttccaaga gtacctaaat
     2101 ctccagtagc aattatatca aagctatttg ggtcatcatt agtatcttca aagtaagagt
     2161 agatagtatc tattgctgct ggagccatag ctggtcccat attattacca tcatcaatac
     2221 cctcatctat tactttacca acagtaacat actttacttt tggaccgtct ccattaggag
     2281 ctaataatac acttcctgct cctgtaactg tccactgggc tgtcattggt ttttgatttc
     2341 caagttcaag tgggaatcta aattgtcttt ctgctgtaca atagtgactt gatgtacctg
     2401 acagtactaa atcagcaaaa ccaccatcta taagcataga acctatacaa agaccttctg
     2461 ccattgtaga acaagctcca tatataccta aaaatggaat agccagttct cttgctgcaa
     2521 aggatgctgg aactatttga tttattaaat cgccacctag catataattt acatctgaca
     2581 attttttacc tgccttttgg atagcttgtt gagatgcagt ctctaccatt ttactttcag
     2641 ctaattccca agtcttttca ccatataaat cgtcagtcat aatcatatca aaatagtcct
     2701 ttagtggtcc ttcaccttcc ttaggaccta ctatagttcc agttgatatt atggttggtt
     2761 tattttctaa cttgactgtt ctttttccaa ttcttttatt tttcatattt tcacaccttt
     2821 ttaattttgc attttactat tcaaattttt aatattaatt aaaaatatta attaaatctg
     2881 cagaattatt tttcaattag ttactttagt agctattttc agattttata ccttcttcat
     2941 aattaataaa atttaaatta aataaaaatc atctaaaaca tcttaaaaat ataataaata
     3001 attccacata aaatagaaga acctatccca taaactaaaa ctggtcctgc aattttaaat
     3061 aagtttgcac ctacacctaa tacatagcct tctcttttat actccattgc tggagatact
     3121 attgagttag caaaacctgt tattggaatt atagaaccag ctccagctct ttttcctatt
     3181 aggtcgtaaa ctcctaatcc agttaaaaat gcccctataa atattaatgt tattgatgtt
     3241 gcagaactag cacctagttt atcaagacct ccaactttca tatacaaatc atttattgct
     3301 tgccctatca tacaaataat tccacctaca ataaatgcaa gtgtatagtt ttttaaatat
     3361 gtaggttttg ggcttatttg gtctacatat tttttataat ttttatccat actaaacacc
     3421 tctctaaagt attatttgtt aattttaaaa aaataaacct attatttatt ttgactatct
     3481 aaagtttggt caaaaaataa aaaagatgcc taataaagaa aaaatctatt tttccataaa
     3541 gacaccttgt ttttatattt ttactttttt aatgcttttt attattttaa tatcttttaa
     3601 taatttaaaa acttaacata aaaagttatt acat
//
LOCUS       DAMJVK010000194         3569 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.199, whole genome shotgun sequence.
ACCESSION   DAMJVK010000194 DAMJVK010000000
VERSION     DAMJVK010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.199"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(157..1428)
                     /gene="serS"
                     /locus_tag="PC414_003751"
     CDS             complement(157..1428)
                     /gene="serS"
                     /locus_tag="PC414_003751"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDF2466098.1"
                     /translation="MLDIKRIRENLDDIKKAMERRGEREFDLDAVVELDNKRREILQE
                     VEVMKNELNVNSKKIPQLIKEGKDVTEEKARLKELSDKIKGIDEKVKEVEAKMEYTLM
                     RIPNVPHPEVPQGETDEDNVQIRTWGEPTKFDFEHKAHWDIGTGLGILDFERAGKITG
                     SRFTLYRGLGARLERSLMNFFLNTHTAKHGYTEVLPPFMANRNSFIGTGQLPKFEEDM
                     FKIEGLEYFLIPTAEVPVTNIHANEILDVAELPIKYCAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVELVKFVKPEDSYNELESLTHDAETMLQMLGLPYRVVKICTGDLGFTAAFK
                     YDLEVWMPSYNRYVEISSCSNFEDFQARRAGIRFKRDKKSKAEYVHTLNGSGLAIGRC
                     LAAILENYQQADGSVVVPEELRPYMGVDVIK"
     misc_binding    complement(1554..1759)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1887..2768)
                     /locus_tag="PC414_003752"
     CDS             complement(1887..2768)
                     /locus_tag="PC414_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HDF2466099.1"
                     /translation="MNFLSLAKTASTNSNITKRTLENMLDNFSDNIPNIIYAIIVFVI
                     GIYISKIVRRMVSKFLIKYGMSKGVNNFIVYGIYISMLSIISLISLGIIGIQTTSVVA
                     VLGAAGFSIGLAFKEILSNLGSGMIILFFKPFNIGDYIQGSGVEGTVSDIQIFSTVLK
                     TPDNKTIIIPNFQLTSNNIINYTHQNKRRIDFSYNISYDSDIDVVKSILNEIFTNEKR
                     ILNDPKPIIGLNSIGNNTMQIVARPWVKTDDYWDVYFDVMEKVKNKFDENDIKVPFVP
                     SSLLFNNTGNLNTMDKR"
     gene            complement(2780..3289)
                     /locus_tag="PC414_003753"
     CDS             complement(2780..3289)
                     /locus_tag="PC414_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HDF2466100.1"
                     /translation="MNSNERRSKLIDILKESKHPVKGGTLAELLNVSRQVIVQDIALI
                     RARGFEIIATPQGYIIYNQPYFTKQIKCKNHKDSKEIYDELKIIVDLGGIIKDVIVNH
                     PTYGQITAELNIYSKMDIDNFMKKVETNEFKQLSVLTECSHIHTIEAIKEETIEAIVK
                     ELKDNGILS"
     gene            complement(3415..3531)
                     /gene="rrf"
                     /locus_tag="PC414_003754"
     rRNA            complement(3415..3531)
                     /gene="rrf"
                     /locus_tag="PC414_003754"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1140 a          628 c          352 g         1449 t
ORIGIN      
        1 atttagtgtt ttatgatgaa aattctgctg tgtaaattaa cataaaaaaa ctatgaaatt
       61 aatatttata ataatttcat agttttttta tttattgttt actcataata tattctcttc
      121 atcaaagcat actctatata tacaattaat ataaatttat tttataacat caacacccat
      181 ataaggtctt aattcttctg gaactactac agaaccatct gcctgctgat aattttctaa
      241 tatagcagcc aaacatcttc ctattgctaa tccagaacca tttaatgtat gaacatattc
      301 tgcttttgat tttttatctc ttttaaatct tattcctgct cttcttgctt ggaaatcttc
      361 aaaatttgaa caagaagata tttctacata tctattgtaa cttggcatcc aaacttctaa
      421 atcatattta aatgcagctg taaatcccaa atctcctgta catattttta caactctata
      481 tggaagacct aacatttgta acatagtttc agcatcatga gttaatgatt ctaattcatt
      541 ataagaatct tctggtttaa caaatttaac taattcaact ttattaaatt gatgttgtct
      601 aactaaacct cttgtatctc ttcctgctga acctgcttca gatctaaaac aaggagtata
      661 tgcacagtat ttaattggaa gctctgcaac atctaatatt tcattagcat gtatattagt
      721 aacaggaact tcagctgttg gtattaagaa atactctaat ccttctattt taaacatatc
      781 ttcttcaaat ttaggaagtt gaccagtccc tataaaacta tttctattgg ccataaatgg
      841 aggtaagact tccgtatagc catgcttagc tgtatgagta tttaaaaaga aattcattaa
      901 agatctttct aatctagctc ctaatcccct atataaagta aaccttgacc cagttatttt
      961 ccctgctctt tcaaaatcta aaatacctag acctgtacct atatcccaat gcgctttatg
     1021 ctcaaaatca aatttagtag gttctcccca agttcttatt tgaacattgt cttcatctgt
     1081 ttcaccttgt ggtacctctg gatgaggaac atttggtatt ctcattaatg tatattccat
     1141 ttttgcttct acttctttta ctttctcatc aatacctttt atcttatctg ataactcttt
     1201 caatcttgcc ttttcttctg ttacatcttt tccttctttt attaattgag gtattttttt
     1261 agaatttaca tttaactcat ttttcataac ttcaacttct tgaagtattt ctcttctttt
     1321 attatctaat tccactacag catcaaggtc aaattctctt tcacctcttc tttccatagc
     1381 ttttttgata tcatctagat tttctcttat tcttttaata tctaacatat taattcctcc
     1441 taataatttt gataatataa gttataattc caacttaatt tacaaacatt atacttgtaa
     1501 actcttagtt taaacaaatt ttaaaaataa aaaaagacct ccgtttaccc tataaaaggg
     1561 acgaaaatct tttatccgcg ttgccaccct aattgactat actaaaatgt ataatcctct
     1621 cagacctata acgtaggtaa acgtctagct ttaactagaa gctctagagt agattcaaaa
     1681 accattgtaa tgagttttca ccatccactc attctctgaa tacaatctgt tttttactat
     1741 tttccttcac agctttttaa ctaattgttt tatttgataa tcattatatt atatatatta
     1801 acatctttca atactattat tcaaattttt ttagaatttt ctttaatttt ttatattaaa
     1861 ttcatttttt ctatttaatt aaaaccttat cttttatcca ttgtatttag attacctgta
     1921 ttattaaata ataatgaaga aggaacaaat ggaactttta tatcattctc atcaaattta
     1981 ttttttacct tttccattac atcaaaatat acatcccagt aatcatcagt ttttacccaa
     2041 ggtctagcaa ctatttgcat ggtgttattt cctatagagt ttaaacctat tataggcttt
     2101 ggatcattta atattctttt ttcgtttgta aaaatttcat ttaatataga cttaactaca
     2161 tctatatcac tatcatatga aatattatat gaaaaatcta ttcttctttt gttttgatgt
     2221 gtgtaattaa ttatattatt acttgtcagt tgaaaattag gtataattat agttttatta
     2281 tctggcgtct taagaacagt gctaaatatt tgtatgtcag aaactgtacc ttctacacct
     2341 gaaccttgaa tataatcacc tatattaaat ggcttaaaaa ataaaataat cataccagag
     2401 cctaaatttg ataatatttc tttaaaagct aaacctatac taaatccagc agctcctaac
     2461 acagctacaa ctgatgtagt ttgtatccct ataataccta agcttatcaa tgatataata
     2521 gataacatag atatatatat tccatataca ataaaattat ttacaccttt actcattcca
     2581 tatttgatta gaaactttga aaccatcctt cttactattt tagatatata aatacctatc
     2641 acaaatacaa ttattgcata tataatattt ggtatattat cactaaaatt atcgagcata
     2701 ttttctaaag ttctcttagt tatattgcta tttgttgagg cagtcttagc caatgataaa
     2761 aaattcattc aactcctcct tatgataata ttccgttgtc ctttaactct tttactatag
     2821 cttctatagt ttcttctttt attgcctcaa ttgtatgaat atggctacat tctgtcagaa
     2881 ccgataattg tttaaattca tttgtctcaa ctttcttcat aaagttgtct atatccattt
     2941 tagaataaat attaagttct gcagttatct gaccatatgt tggatgattt acgatgacat
     3001 cttttataat accacctaaa tccacaatta tctttaattc atcataaatt tctttagaat
     3061 ccttatgatt tttacacttt atttgttttg taaaatacgg ttgattatat attatatatc
     3121 cttgaggtgt agctattatt tcaaatcctc tagctcttat aagggctata tcttgaacta
     3181 ttacttgcct acttacattt aatagttcag ctagtgttcc accttttaca ggatgcttag
     3241 attcttttaa aatatctatt aatttacttc ttctttcatt agaattcata ttacaccttc
     3301 tttccaaata aaaactttac ctatactata taattttata ttttatctta ttataaatca
     3361 atattgactt tatattctat ttaatcttaa cttttataat aaataaaaaa gactacgtgg
     3421 ctacgtccta ctctcccagg cagtttccca ccaagtacca tcagcgctag agagcttaac
     3481 ttctgtgttc ggaatgggaa caggtgtatc ctctctgcta ttgtaaccac ataatcttta
     3541 tgcttaatat ttctattaag tttttaata
//
LOCUS       DAMJVK010000195         3498 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.200, whole genome shotgun sequence.
ACCESSION   DAMJVK010000195 DAMJVK010000000
VERSION     DAMJVK010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3498
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.200"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(154..1721)
                     /locus_tag="PC414_003755"
                     /pseudo
     CDS             complement(154..1721)
                     /locus_tag="PC414_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021426623.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iota toxin protein Ib"
     gene            complement(1696..1839)
                     /locus_tag="PC414_003756"
     CDS             complement(1696..1839)
                     /locus_tag="PC414_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2466101.1"
                     /translation="MYVYYFESPEKFAFNKVIRTKNQNEISLEKFNEFKETIQDKLLP
                     YNR"
     gene            complement(2057..2239)
                     /locus_tag="PC414_003757"
     CDS             complement(2057..2239)
                     /locus_tag="PC414_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2466102.1"
                     /translation="MKKFRKHKSISNCISILLILYLTLGSLLPNNIYAQDLQSYSGKV
                     CNTTYKAPIERSEGFF"
     gene            complement(2628..3374)
                     /gene="cdtR"
                     /locus_tag="PC414_003758"
     CDS             complement(2628..3374)
                     /gene="cdtR"
                     /locus_tag="PC414_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family response regulator CdtR"
                     /protein_id="HDF2466103.1"
                     /translation="MDILIFDNDVCFGMKLKEKINNILIKEGFDNDVVRLYHNANLLL
                     KELTEKNKVRIYFIVVDAKYKISNELCDGLWIAQKIRESDYISPIIFLTNHIEMILGI
                     FDYRLEVMDFILKHDMEIAESKIKACIKIAHKRYIKEKNYRSNFFTIYSDFSLWKISF
                     DEVIYFETSTIPHKIKLVTTSRIFEFYKSLKSLPDLDACFIRVHKSFVVNKYHIVSLD
                     LKKNNIKMSNGHICRISNTYRNILKNIIKT"
BASE COUNT         1170 a          454 c          381 g         1493 t
ORIGIN      
        1 gataattatc tttttaatac aatatagttc taaaaaagaa tatattagaa gaagataata
       61 tttaaataaa aaatacctta taaagttaat tttttaatac tttataaggt aaatacatat
      121 tgtattgtgg ggataaattt aaatcatttg atactaatca acactaagaa ccaataactc
      181 tctatcatct tgagtaattg tatatattct taatttcttg tatgtcataa tattttctcc
      241 acctgtaaaa taactcctaa gattaacaca atcagtttta ggttgatttg tatctcctaa
      301 ataattacgc aattctttat ctttagtttc aatatcttta aatccaaaat attgtaaagt
      361 agcttcaact ctatattttt ggacataatc gagagcttct ttattagttt atgttggtgt
      421 aaaatacata ccatttatat aagcctttat tgtacttgga ttaaatttaa aatctgcaga
      481 atatatttta tgtgtaacta ttatttcttg aacagttgga attttaactt ctggttcatc
      541 aagtatttca ggagtactat ttagctctgt aatagataag ttatctaagt atattgtatc
      601 actatcaatt aatgctatat ttatattaga agaatctttt tcagtagttt caaattcata
      661 actaaatttt gtatatcctt gttctagtac caaataatcc gttttttctt cttttacttt
      721 tatatttaca attattgaat tatatgttaa aggatttttt gaattttact aaaaacataa
      781 cgtttataag gttttagttc agacataggt attttaatct tcgtctcacc atttaatcta
      841 tttgctgagc cttttaaacc atcttgattt gtagtattaa cattactcca tatactaggg
      901 taattattgt aatcatcaaa attagtaaag taagttggtg tttttataag tatattcatt
      961 cctctttcaa gtttaacatt acatatttgt ttatcagaca aagtttttaa actgtcttta
     1021 atcttattgc ctatattatc atcaaatatg agttcaacac aactttcatc tattggtata
     1081 ttattaaaat ataacaaacc atctttttta gtagtgccaa aagttttttc aattgcttct
     1141 ccaattgtaa gttcaggtgt tttatcttct gggacctgta aacttttagc agtaattctt
     1201 cttttgtaag attcattttc tgtatctaat ataatagata cagaaatact gtcaatttga
     1261 cctatatcgt cttaccaact atttcattct gttactattt gtccagaacc attttttgta
     1321 ccaaaatttc cacttacttg tgttgtttct aatttaattt gctttccatc atctaatttt
     1381 tttaattaat tataatttat aggaatcagt ctagaactaa attgatacat tgtgttaaga
     1441 gccataggtg aaaatccttt tttgagataa gtatttccag gagatagatt attgccaatt
     1501 tgattttctt gtgctttgat agttgataat gcatctccat ctaacactaa atttgttgtt
     1561 ggtgtcactt tgtacatagg tgcagtacct gtgttgtagt atctaatatt tgcatttata
     1621 tatgcagatt ctcctttgtt tatacttaat ccagtatttc atgattatcc attgctattt
     1681 tgaacagcag ttgaattatc tgttgtatgg taataatttg tcttgtatag tttctttaaa
     1741 ctcattaaat ttttctaatg aaatttcgtt ttgatttttt gttcttatta ctttattaaa
     1801 tgcaaatttt tctggagatt caaaataata tacatacata ggtttatcta ttttattttt
     1861 tgatatagca tttcgaagtt ctctatattc tttttctcaa gaatttgctt ctatttgata
     1921 atcataaaaa taatttcttg tctgagaata tttgcttatc tctatagaat cttttttata
     1981 tgatcctaat acttcttttt cagatttttc aagtttttga tcttttcttt ctcattcttt
     2041 agccttttct ttatctttaa aaaaatcctt ctgatctttc tataggagcc ttgtaagtag
     2101 tattacaaac ttttccacta tagctttgta aatcttgtgc ataaatgtta ttaggtaaca
     2161 aactacctaa agttagatat aatatcaaca atatagatat acaattacta atacttttat
     2221 gtttcctaaa ttttttcatt tattctccct acaaatatta gtttagttaa cttttgaatt
     2281 ttaatttatt tagattatat aatctaaata agaatttttt atataaattt cataaaatat
     2341 gtaggtatat atcataaaaa tatgtattta tatgatatgt cagtacaaaa taacaactga
     2401 atattttaaa gagactattt cgtactttta tgatttaatg agctatctta tcgaatcata
     2461 atctaggaac aattgttata atatctaata gtagttatat atgatatagt cagtatgtgt
     2521 tttttcacaa tatgattatt atatagagga ttcttaggga ttactctttt tgtataaaaa
     2581 agagatggtt caaattttta tcttgagaca tctccttttt ctatttatta tgttttaata
     2641 atattcttta aaatatttct atatgtatta gatatacgac atatatggcc attactcatt
     2701 ttaatattat ttttctttag gtcgagagaa acaatatgat atttattaac tacaaaagat
     2761 ttatgtacac ggataaagca tgcatctaaa tctggtaaag actttaaact tttatagaat
     2821 tcaaatattc tagaagtagt cactaatttt attttatgtg gaatagtgct tgtttcaaaa
     2881 tatataactt catcaaatga aattttccat agtgagaaat ctgaatagat agtaaaaaaa
     2941 ttagagcgat aatttttttc tttaatgtat cttttgtgag caattttaat acaagctttt
     3001 attttacttt cagcaatttc catatcatgt tttagtataa agtccataac ttctaaccta
     3061 taatcgaaga tccctaaaat catttctata tgatttgtaa gaaatatgat tggactaata
     3121 taatctgatt ctctaatttt ttgtgctatc cataatccat cacataattc attacttatc
     3181 ttatattttg cgtctacaac tataaaatat attcttactt tatttttttc agtaagctct
     3241 tttaataaca aatttgcatt atgatataat cttacaacat cattatcaaa accttctttt
     3301 attaaaatat tatttatttt ttcttttagt ttcattccaa aacaaacatc attatcaaag
     3361 attaatatat ccactaaata ccctcctata aaaaattcaa aagtaaaaaa acaaaagtat
     3421 tttatagaaa atacataaaa taacactagg agataaataa gattaaggat agttagaatt
     3481 aaggtatatt tatgcttg
//
LOCUS       DAMJVK010000196         3458 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.201, whole genome shotgun sequence.
ACCESSION   DAMJVK010000196 DAMJVK010000000
VERSION     DAMJVK010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.201"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            93..1085
                     /locus_tag="PC414_003759"
     CDS             93..1085
                     /locus_tag="PC414_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDF2466104.1"
                     /translation="MELKDRSLVNNQYKGKIKSANLSVDYEINQVMKDIDKLGLNTVN
                     VPIIINVESINSDIMSIDNNSKEKAIKLIKKLNKKGISTILEAYPWIENGKLYETDWN
                     PINKKRFFYTWQNAILNELIIDVANPLDVNVLNIGSNFVHLEEYQQNWGEIIDFVQSK
                     FDGLVTYRTNWWYTKKDDLSSKLFYEQKLNNNFFDKLDFISIAAYFELSNKPVNTVDE
                     LISALHSSTVNNRGQNIKQEIYNLYKAHRKPIFFGELGFSNRESASSQPWNHTPSKAV
                     NGEEQARCFEAYKKVFENEDWINGFSVFCVGKIDDEKNFYPSKESIKVIKSWYE"
     gene            1139..2263
                     /gene="wecB"
                     /locus_tag="PC414_003760"
     CDS             1139..2263
                     /gene="wecB"
                     /locus_tag="PC414_003760"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HDF2466105.1"
                     /translation="MNDIKVMTVFGTRPEAIKVAPLIKELEKRENIKSIVCVTAQHRE
                     MLDQVIETFNINVDYDLDIMEKGQSLNDITCKILNKLPLILNKENPNIILVHGDTTTT
                     LATSLTAFYNKTLVGHIEAGLRTYDKYSPFPEELNRQLTGIIADMHFAPTNLARKNLI
                     SEGKPNNNIFVTGNTAIDALKMTIKENYNHPIIDEIGNDRMILLTSHRRENLGKPMKN
                     IFRAIKRIVDDFEDVQIVYPIHLNPKIRTIADEIFGKFPEKIHIIEPLDVADFHNFLN
                     KSYMIMTDSGGIQEEAPSLGKPVLVLRDKTERTEGIEAKTLKLVGTNEDRIYNSVSDL
                     LINKDNYVQMSKASNPYGDGNASKYIVDIIIKKFNCKYLN"
     gene            2341..3315
                     /locus_tag="PC414_003761"
     CDS             2341..3315
                     /locus_tag="PC414_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDF2466106.1"
                     /translation="MARKLIKNLGKSKSVKRVKLLFKKIFITVFIVASIVAIFNITKY
                     FEELYKVRDLKSTKIEYYMDVADEAGDGKVQLSWKALLAIDMVIHDEDLSNIKKKDTL
                     DIGEKFIVEDKNGKGEKVYKVKKFNKVLSELKFDSSQKSRARKYMKDLEYTYLGNKQL
                     DSSDEKIKFIKKLEDSAIREYIDYGILPSITIGQAILESGWGNSKLTKQSNNLFGIKA
                     DKAWKGKSVEISTSEHYNEKIVASFRSYNSLQDSVKDHSLFLINNKRYRKHGLFEAKD
                     YISQAQALENAGYSTAEDKKGNRIYAELLIDVIRSYNLQLIDNKVETK"
BASE COUNT         1445 a          335 c          606 g         1072 t
ORIGIN      
        1 tgaggaaata tatgaatgaa tttaaattaa aaagaagtat tttaatctca ttaataatct
       61 taatttttat atttatagtt tactttgtaa gtattgagtt aaaagatagg agtctagtaa
      121 ataatcaata taaaggtaag attaaatcgg ccaatttatc tgtagattat gaaataaatc
      181 aagttatgaa ggatatagat aaattgggat tgaatacagt aaatgtacct ataattataa
      241 atgtagagtc cattaattct gatattatgt caatagataa taatagtaaa gaaaaggcaa
      301 ttaagttaat aaaaaaatta aataaaaaag gtatttcaac aatattagag gcatatccat
      361 ggattgaaaa tggaaaattg tatgagactg actggaaccc tataaataaa aaaagatttt
      421 tttatacttg gcaaaatgcc atattaaatg aacttataat tgatgtagca aatccactgg
      481 atgtaaatgt tttaaatatt ggttctaatt ttgtacattt ggaagaatat caacaaaatt
      541 ggggtgaaat aatagatttt gtacaatcca aatttgacgg tcttgtaacg tacagaacta
      601 attggtggta tacaaaaaaa gatgatttaa gtagtaagtt gttttatgaa caaaagctga
      661 acaataattt ttttgataag ttagatttta tatctatagc agcatatttt gagttaagta
      721 ataaacctgt taatacagtg gatgaattaa tttctgcctt acactcatct actgtaaaca
      781 atagaggaca aaatataaaa caagaaatat ataaccttta taaagctcat agaaaaccta
      841 tattttttgg agaattaggt ttttcaaata gagaaagtgc aagttctcag ccgtggaatc
      901 atactccttc aaaagctgta aatggcgaag aacaagcaag gtgttttgag gcatataaga
      961 aagtttttga aaatgaagac tggataaatg gtttttctgt gttttgtgta ggtaaaatag
     1021 atgacgaaaa aaatttttac ccatcaaaag agagtataaa agttattaag tcttggtatg
     1081 aataattttg aaagtgtaga atttatatag attaaataga tgattaggag agatataaat
     1141 gaatgacatc aaggttatga ctgtatttgg aactagacct gaagctatca aagtagcacc
     1201 tttaataaag gaacttgaaa aaagagagaa tataaaaagt attgtttgcg ttacagctca
     1261 acatagagaa atgcttgacc aagttataga aacctttaat ataaatgtag actatgattt
     1321 ggatataatg gaaaaaggac aaagcctgaa tgatataaca tgtaagattt taaataaact
     1381 tcccttgatt ttaaacaagg aaaatccaaa tataatattg gtacatggag atacaaccac
     1441 aactctagct acaagtttga ctgcatttta taataaaact cttgttggtc atatagaagc
     1501 tgggcttaga acctatgata aatattctcc ttttccagag gagctaaata gacaacttac
     1561 aggtattatt gctgatatgc actttgctcc tactaatttg gctagaaaaa acttgatttc
     1621 tgaaggaaaa cctaataata atatatttgt aactggtaat actgctattg atgctttaaa
     1681 gatgacaata aaagaaaact ataatcatcc tataattgat gagattggta acgataggat
     1741 gattttgtta acctctcaca gaagagaaaa tttgggtaaa cctatgaaaa atatattcag
     1801 agctataaag cgaatagtag atgactttga agatgtacag atagtatatc ccatacattt
     1861 aaatcctaaa atacgaacta tagctgatga gatatttgga aaatttcctg aaaaaattca
     1921 tattatagag cctttagatg tagctgattt tcataacttt ttaaataaat cttatatgat
     1981 aatgactgat agtggaggaa tacaggaaga agctccatca ttaggtaagc cagttcttgt
     2041 gttgcgtgat aaaacagaaa gaacagaagg aattgaagca aagacactta aattagtagg
     2101 tactaatgaa gatagaatat ataattcagt gagcgattta ttaataaata aagataacta
     2161 tgttcagatg agtaaagcat ctaatccata tggagatgga aatgcaagca aatatatagt
     2221 ggatataata attaaaaaat ttaattgcaa atatttaaat tagataaggc ctaaaacatt
     2281 aactaattga tataagattt atgataatat taaggaatac aatttttaag ggggagtaat
     2341 atggctagaa aattaataaa aaatttgggt aaaagtaaaa gtgtaaagag agtgaaactt
     2401 ttatttaaga agatttttat tactgtattc atagtagcaa gtatagttgc tatttttaat
     2461 ataacgaaat attttgagga attatacaaa gtaagggatt taaaaagtac taaaattgaa
     2521 tactatatgg atgtggctga tgaagcagga gatggaaagg tccaattaag ttggaaggcc
     2581 ttacttgcta tagacatggt aatacatgat gaagatttaa gtaatataaa aaagaaagac
     2641 acattggata taggggaaaa gtttatagta gaagataaaa atggtaaagg cgaaaaggtg
     2701 tataaggtaa aaaagtttaa taaggtatta agcgaattga aatttgactc ttctcaaaaa
     2761 agtagagcaa gaaaatacat gaaagattta gaatacacat accttggaaa taaacaatta
     2821 gatagtagtg atgaaaaaat taaatttata aagaagttag aagactcagc tataagagaa
     2881 tatattgatt atggaatatt gccctctata acaattggac aagctatatt agaatctggt
     2941 tggggaaatt ctaaacttac aaaacagagt aataatttat ttggtataaa agcagataaa
     3001 gcatggaaag gaaaaagtgt agaaatttca acttcagagc attataatga aaaaattgta
     3061 gctagtttta ggtcgtataa ttcattacaa gattctgtca aagaccacag tttattttta
     3121 attaataata aaagatatag aaaacatggg ttgtttgagg caaaagatta tattagtcaa
     3181 gctcaagcat tagaaaatgc aggatatagt acagcagaag ataaaaaagg aaatcgtata
     3241 tatgcagagc tattaataga tgttattagg agttataatt tgcaattgat agataataaa
     3301 gttgagacaa aataattgtt aactttatat gaggagataa caagataaga tttttttcta
     3361 caaaaagaat agaaaaattg taaaaaaaac taaaaaaatg tattgacttg ttatttaaaa
     3421 ggtgatatta ttattactgt actcgaaaga gaacaaaa
//
LOCUS       DAMJVK010000197         3360 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.202, whole genome shotgun sequence.
ACCESSION   DAMJVK010000197 DAMJVK010000000
VERSION     DAMJVK010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.202"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(380..1102)
                     /locus_tag="PC414_003762"
     CDS             complement(380..1102)
                     /locus_tag="PC414_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004398740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDF2466107.1"
                     /translation="MITIKNLSKSFGDLNVLKNIDLEIAKGEIMVIVGPSGSGKSTLL
                     RCMNLLEIPTGGEIIFEGKNLVDKKTNIDEVRQNIGMVFQNFNLFPHKTILDNITLAP
                     IKLKKMTKEEAEKKAEILLSRVGLLDKKDSYPSQLSGGQKQRIAIARALAMEPDMMLF
                     DEPTSALDPEMVNEVLDVIKELAKEGMTMAIVTHEMGFAKEVADRVIFIDGGSILEDN
                     TPEEVFGNPKHERTKAFLAKVL"
     gene            complement(1161..1838)
                     /locus_tag="PC414_003763"
     CDS             complement(1161..1838)
                     /locus_tag="PC414_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDF2466108.1"
                     /translation="MSLDFSFLSRFGTSFLEGTGVTVSISLVALCFGFIIGIIICMAK
                     ISKSKVLRAISSIYIEVLRGTPLLVQIYIVWFGLPQLGIRFPMLFGIPSEFIASAFAL
                     SVNSGAYVAEILRSGIQSVDNGQMEASRSLGLNYWSTMRYIIIPQAIKNILPSLANEF
                     ITLVKESSIISVIGVVEIMRTADIVKNAAFRALEPLIVAAAIYFVITFTLSRLVGLLE
                     KKLSVSN"
     gene            complement(1866..2681)
                     /locus_tag="PC414_003764"
     CDS             complement(1866..2681)
                     /locus_tag="PC414_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDF2466109.1"
                     /translation="MKVFKKLLSLGLVLGLTLSLVGCSGGGEKTKLEQIKDNGKLVVG
                     TSAEFPPFEFHKVVDGKDSIKGFDIMLAEEFAKELGVKVEIKDMSFDGLIGALNADQV
                     DIVLAGMSPTPEREKSVDFSELYYLSRNAVIVKDADIDKVKTEDDLKKLRVGVQAGSI
                     QEEYVVNTLKMTTTKSLKAIPDLITELKNGNIDAVVTNEAVSLINVKKYDGIKMANTE
                     VGKDVTEGMAAAIKKSDNNKDFIELLNKKIKELQDGKKIEEFLNEASTEAASN"
     misc_binding    complement(2907..3117)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         1053 a          620 c          339 g         1348 t
ORIGIN      
        1 tatttttact ataaaagtta aatttataag cttacttgtt ataaaaataa cttttataat
       61 ttatttcaaa aaaaatattg atttttgtgt cgcattttgt tataataaat taaggttatc
      121 cccctggtaa ccctagttag ttaatatatc tattcccaat accctttcat aaggatatat
      181 tttatattta ttaaaaatcg gattagtaac ggaaattaat tcggtggaaa aaaagagctt
      241 accccgagct cttttttttc tgcaaaaaat cgtaaaaata aagttaatat taatataact
      301 ttaattttaa aattaatatt aaccatcact aaaaaaggat gtattgaatt tctaatatca
      361 ataacatcct ttttatatat tatagcactt ttgctaaaaa tgcttttgtt ctttcatgtt
      421 ttggatttcc gaaaacttct tctggagtat tatcttcaag tatacttcct ccatctataa
      481 aaataactct atctgcaact tccttagcaa atcccatctc atgagttaca attgccattg
      541 tcataccttc tttagcaagt tcttttataa catccaaaac ttcgtttacc atttctgggt
      601 caagagcaga tgttggctca tcaaacaaca tcatatctgg ttccatagca agtgctctag
      661 ctattgctat tctttgcttt tgtcctccag ataattgtga tggatatgaa tcttttttat
      721 ctaaaagacc aactctactc aacaaaattt ctgctttttt ttctgcttct tccttagtca
      781 tcttttttaa tttaattggt gctaatgtta tattatctaa aatagtctta tgtgggaata
      841 aattaaagtt ctgaaacacc atacctatat tttgtctaac ctcatcaata ttagtttttt
      901 tatcgaccaa attttttcct tcaaatatta tttctccacc tgtaggtatt tctagtaaat
      961 tcatacatct aagaagtgta cttttaccag aaccacttgg acctactata accattattt
     1021 cgcctttagc gatttctaag tctatatttt ttaatacatt taaatctcca aaagatttac
     1081 ttaaattttt tattgtaatc atattatcac tttccttttt actattctat gttttcccta
     1141 aatgctaaac atgctctcat ctagttagac acagatagtt tcttttctaa taatccaact
     1201 aatcttgata gagtaaatgt tataacaaag taaattgctg ctgctactat taatggctct
     1261 aatgctctaa atgctgcatt ttttactata tcagcagtac gcattatctc aacaactcct
     1321 attacagata ttattgaaga ttcttttaca agtgtaataa actcatttgc taatgaaggt
     1381 aatatatttt ttatagcttg aggtattatt atatatctca tagttgacca ataatttaat
     1441 cctaatgatc tacttgcttc catttgtcca ttatcaactg attgtatacc tgatctaagt
     1501 atctctgcca cgtatgcacc agagtttact gataatgcaa atgcactagc tataaattca
     1561 cttggtatac caaacaacat agggaatctt attcctaatt gaggtaaacc aaaccatact
     1621 atatatatct gtactaataa tggtgtccct cttaaaacct caatatagat tgatgatatt
     1681 gctcttaata ccttgctctt tgatattttt gccatacaga taattatacc tattataaat
     1741 ccaaagcata atgccacaag agaaattgat actgtcacac ctgttccttc caaaaaagaa
     1801 gtcccaaatc tacttaaaaa actaaaatct aaactcataa tttcatctcc cacataactt
     1861 tcttactaat ttgaagcagc ttcagtagat gcttcattta aaaattcttc tatcttcttg
     1921 ccatcttgta attcttttat ctttttgttt aataattcta taaaatcttt gttattgtca
     1981 gattttttga ttgctgctgc cataccttct gttacatctt tcccaacttc agtatttgcc
     2041 atttttattc catcatattt tttaacattt attaaagaaa ctgcttcatt agtaacaact
     2101 gcatctatgt ttccattttt taattctgta attaaatcag gtattgcttt caaagatttt
     2161 gtagttgtca tttttaaagt gttaactaca tattcttctt ggatacttcc tgcttgaact
     2221 ccaactctaa gtttctttaa atcatcttca gtttttactt tgtctatatc tgcatcttta
     2281 actataactg catttctact taaataatat aattctgaaa aatcaacact cttttctctc
     2341 tctggtgttg gagacatacc tgcaagaaca atatcaactt ggtctgcatt taaagcacct
     2401 attaatccat caaatgacat atccttaatt tcaaccttta caccaagctc ttttgcaaat
     2461 tcttcagcca acattatatc gaatcctttt attgaatctt ttccatctac taccttatga
     2521 aattcaaatg gaggaaattc tgcactagta ccaacaacta attttccatt gtcttttatt
     2581 tgttctaact ttgttttttc tcctccacca ctacatccta ctagcgataa tgtcaatcct
     2641 aatactaatc ctaaacttaa taatttttta aatactttca ttttaactac ccctctcttt
     2701 taattataat ttccctcaga ttttaatact taaatgtttc gagttacaaa aaaagtctct
     2761 cagtctttaa tgactaagag acgactttac cgcgttacca ctcttattga taaatactta
     2821 tattactttc aaaatttgtg actgttagca tttaagctgt atggatactt ttatttttaa
     2881 atcaaaaaag tcccttagct ttacagctaa gagacgaatt atatccgcgt taccactctt
     2941 tttgataagt aagtacttat cctctcaatc ccttaaggcg ggtaaaccaa ttatcatact
     3001 aaagtttctg ataatctcct ccagagctct cttcacatag acttcattgc taggcttaca
     3061 ccaaccccta actcgctgta aatccatata tgctactttc tctatcattg gatttgtttc
     3121 cttaaattgt taatacaatt gtacttcctt ataatatttt tgtcaataag ttttttattt
     3181 ttttaataat ttttacttgt aaattatcta aatactactg tttatattta ttgatattag
     3241 ttttgtaatt ataaatagta tatttcatat ttttagatat ttcttaaata atactaaata
     3301 atatctttca cattgttgct ttttcttaaa caatactaaa taatatcttt cacattgttg
//
LOCUS       DAMJVK010000198         3278 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.203, whole genome shotgun sequence.
ACCESSION   DAMJVK010000198 DAMJVK010000000
VERSION     DAMJVK010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.203"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            81..692
                     /locus_tag="PC414_003765"
     CDS             81..692
                     /locus_tag="PC414_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HDF2466110.1"
                     /translation="MINDIKNKFKNYKPYINGYKNMKRASVLIPIVEINNTHYILFEV
                     RSKNLKHQPSEISFPGGKIESGETPYEAVIRETCEELGTFSDNIEVISSLDLLITPVK
                     FIIHPYLGYIKNINNLNINKDEVDHTFLVPIKYLLENPPNTYSNTVKVFPDENLPYGS
                     IPNQKDYKFESGSYPILFYKYKDYVIWGMTAKILENFLNFLSE"
     gene            990..1328
                     /locus_tag="PC414_003766"
     CDS             990..1328
                     /locus_tag="PC414_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HDF2466111.1"
                     /translation="MFAVGFVVIIALAFFILSVLLFNGKASMLLAGYNSMSKKEKARY
                     DKKKLCRSSSIVTFIVSIMLFIMAFLGYRIESGQMDEKKMVPFSIIFIIVILSSVFFN
                     ILYSNKKCKK"
     gene            complement(1735..3171)
                     /gene="miaB"
                     /locus_tag="PC414_003767"
     CDS             complement(1735..3171)
                     /gene="miaB"
                     /locus_tag="PC414_003767"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HDF2466112.1"
                     /translation="MSKRKQVTIPIEKIQEQDKYIEQIHRQNEEYFNRTGKRKLVFTQ
                     TFGCQMNEHDSEKLCSMLEEMGYQMSMMVEESDLIIYNTCAVRENAELKVYGNLGQLK
                     HLKGKNPDMKIAVCGCMMQQPHVVEELRKKYKHVDLIFGTHNLYKFPQLLTESINSDK
                     MLVDVWDVDGEVIEGLRSNRKFELKAFVNIMYGCNNFCTYCIVPYTRGRERSRTPEDI
                     INEIKELVANGTKEITLLGQNVDSYGKTLENPVSFSELLRKVNDIEGIERVRFMTSHP
                     KDISDEVIYAIRDCDKVCEFLHLPIQCGSSSLLKKMNRHYTKEYYLEIIEKAKKEVPG
                     IAFSTDLMIGFPGETEEDLLDTLDVVEKVRYDSAFTFIYSKRQGTPAAKMENQIPEDI
                     KHDRFNRVLEAVNRISAEINDGYKDRIVEVLVEGRSKNNENKFAGRTRQNKLVNFEGG
                     NDDLIGKLVMVKITEPRTFSLNGILVNN"
BASE COUNT         1134 a          474 c          371 g         1299 t
ORIGIN      
        1 taattagata aatcataatt atatattatt gttaaaactc aattatataa tttttaataa
       61 tattatttta ggaggtaaaa atgataaacg atattaaaaa taaattcaaa aattacaaac
      121 catatataaa tggatacaag aatatgaaga gagcatctgt tttaattcca atagtagaaa
      181 taaataacac acactatata ttatttgaag ttcgctcaaa aaatttgaag catcaaccta
      241 gcgaaatttc ttttccaggt ggtaaaatag aatcaggtga gactccatat gaagctgtta
      301 ttagagaaac ttgtgaggaa cttggaactt ttagtgacaa tatagaagta atatcaagtc
      361 ttgacctcct tataacacca gtaaagttta tcatacatcc atacttagga tatataaaaa
      421 atattaataa tttaaatata aacaaggatg aagttgacca tacttttttg gttcctataa
      481 aatatctttt agagaatcct ccaaatactt atagtaatac tgtaaaagtt tttccagatg
      541 aaaatctacc atatggttca attccaaacc aaaaagatta taaatttgaa agtggaagtt
      601 atcctattct gttttataaa tataaagatt atgtaatttg gggaatgaca gcaaaaatac
      661 tagaaaactt tttaaatttt ttatcagaat aacactcata ataaattatc aaaattttta
      721 cataagaaat ttataaaagt ttctaaattt aacaattcta aaatatcaat aactatattt
      781 tgtagcactt ttttatttta ttattgtttg aatatttgat ggttttcaat gcaatttcaa
      841 cataaatact atatattagt gtatactgtt gatgaattaa agtatgataa tctatttaat
      901 aaagtatgaa gtaaatgatt ttaatgtgcc tttatggtaa aattactaat aagtaataaa
      961 attaattaaa atatatggag gtccttataa tgtttgctgt aggatttgtt gttataattg
     1021 cacttgcctt tttcatttta agtgtactat tatttaatgg aaaagccagt atgctactag
     1081 caggttataa ctctatgagt aagaaagaaa aagcacgata cgataaaaaa aaattatgta
     1141 gaagttctag cattgttact tttattgtct ctataatgct atttattatg gcgtttttgg
     1201 gatatagaat agagagtggt caaatggacg aaaaaaaaat ggttcccttt tctataatat
     1261 ttataatagt catactttcc agtgtatttt ttaatatcct ctactctaac aaaaaatgta
     1321 aaaaataaat agttgtaagt tgagcagtta attataaaag attgactgct ttttcatgta
     1381 tattgactac tcttattaaa tattttaaca tttaaaaatc tatgtaaaaa taatgaatat
     1441 agtacaaact tctaataatg attttacctt tgtggttaca gtccactata tataatttaa
     1501 tatgataaca gggaattatt aatgacaact aagtaaactt ttataaaata taataaaaca
     1561 aaaatagatg tacaatgaaa ttatatctta tatcaaattt atttttatta atagcaaaaa
     1621 cttacattag cataacatat gacaaattcg cttaaaataa aaatttttaa tataatttgt
     1681 tctatagtac atctattttg attagtttac ataattttat tattttgtac aatattaatt
     1741 atttactaat atcccattta atgaaaaagt tctaggttca gtaattttga ccataactaa
     1801 tttaccaatt aaatcatcat ttccaccttc gaaatttacg agtttatttt gtctagttct
     1861 accagcaaac ttattttcat tatttttact tcttccttca actagaactt caactattct
     1921 atctttatat ccatcattta tttctgcaga aatcctattt acagcttcta acaccctatt
     1981 aaatctatca tgctttatat cttctggtat ttgattttcc attttagcag caggagttcc
     2041 ctgtctttta gaataaataa atgtaaaagc tgaatcatat ctaacttttt caaccacatc
     2101 taaagtgtcc aataaatctt cttctgtttc tcctgggaaa ccaatcatta agtctgtaga
     2161 aaaagcaata cctggaactt cctttttagc tttttctatt atttctaaat aatattcctt
     2221 tgtataatgt ctattcattt ttttcaaaag gctactgctt ccgcattgta ttggtagatg
     2281 taaaaactca cacaccttat cacaatctct aattgcgtat attacttcat cagatatatc
     2341 ttttggatga gatgtcataa atcgaaccct ttcaattcct tctatatcat tcacttttct
     2401 taaaagttct gagaatgaaa ctggattttc aagtgtctta ccataagaat ctacattttg
     2461 tccaagtaaa gtgatttctt tcgttccatt tgcaacaagc tcttttatct cgttaattat
     2521 atcttcagga gttctacttc tttctcttcc tcttgtgtat gggactatac aatatgtgca
     2581 gaaattattg catccataca ttatatttac aaatgctttt aattcaaatt ttctattact
     2641 tctaagacct tctattactt ctccatccac atcccataca tccactaaca ttttgtcaga
     2701 atttattgat tctgtaagta attgcggaaa tttatataga ttatgtgttc caaatataag
     2761 gtctacatgt ttatattttt ttcttaattc ctctacaaca tgtggttgtt gcatcataca
     2821 tccacataca gctattttca tatctggatt ttttcctttt agatgtttta attgtcctaa
     2881 gttaccatat acttttagct ctgcattttc tctaactgca caggtgttat atattattaa
     2941 atcactttct tcaaccatca ttgacatttg gtatcccatc tcttctaaca ttgaacataa
     3001 tttttctgaa tcatgttcat tcatttgaca cccaaaggtt tgagtaaaca cgagttttct
     3061 ctttccagtt ctgttgaagt attcttcatt ttgtctgtgt atttgttcta tatatttatc
     3121 ttgttcttgt attttctcta taggtattgt tacttgtttt cttttgctca cgtctattcc
     3181 tcctagttaa tttcttatat tacacataac cttaattata tcaaataaga aaaatctttt
     3241 aaagatatta ttatttttct agttgtgttt taatagta
//
LOCUS       DAMJVK010000199         3204 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.204, whole genome shotgun sequence.
ACCESSION   DAMJVK010000199 DAMJVK010000000
VERSION     DAMJVK010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3204
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.204"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            180..974
                     /locus_tag="PC414_003768"
     CDS             180..974
                     /locus_tag="PC414_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2466113.1"
                     /translation="MIEVDKLCKSFTRVVKDDKNKSSIRKLKKVKTKKEEFFAVNNVS
                     FKVMEGEIVGILGPNGAGKTTLLRMLGGILTPTSGSINISGYDYSIDRNSAKKEIGYL
                     SGNTKLYGRLSPRELLTTFASLYEMSKEDIEESIENIVKIMDMSEFIDNRIENLSTGQ
                     TQRTSIARCLIHSPKVYIFDEPTLGLDVLSSASIIDFMKNEKLRGKTVLYSTHYMEEA
                     ETLCDKIFMIHNGKIIASGTPQSLKEESGVNNLRDVFIKLARKEDI"
     gene            975..3035
                     /locus_tag="PC414_003769"
     CDS             975..3035
                     /locus_tag="PC414_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF2466114.1"
                     /translation="MRSKIVKYIFKKEMLDILRDKKTLFMMIVVPLLLYPLLMLLMIQ
                     VMNMSASSMNSKDINIAFNNKPNKVLVSMIEDDNLEKDTKNTSNDKGKIRVLDVDNYK
                     KSLEENKIDAYIKMEEKKSSINYQIYINSSQENSSNALKRIETVLDKYKRFTIEKTLS
                     EKGLDVQATLEPITYSTVDVAKTEEVAGYFLGQILPFILIIGVLLGSIYPAIDVMAGE
                     KERGTLETLFTLPISNLELVMGKYMAVSLSAIVTAILNILSMGLTMAFILVSGGISSQ
                     FGFGNFNYGDLVLPIITTLICICLFSMVVSAISMCVCSLAKSFKDAQNYITPLMLIIM
                     IPSYVSMIPNIVLDRVTAVIPVVNISLLIKSVLSFKSDLNLIALVLASNVAFVILAII
                     LLSKMFNSEEILFGNSKSFSFLEKRSNIKKGSIPTVSDGVILYAVGLVLLIYVGSLVQ
                     IKFGMAGIIMTQFMIISLPLLFAYYIKADFKEVFSLTVPKLKHVFGSIVLWIGGYILI
                     MIITQLILFLFPQNMEVAESLNKALFIKDSVLLNLLAIALLPAICEEMFFRGFIFSSF
                     SKSKDKNKSIKLAIICSGVLFGIMHMDFIRIIPTSILGIIFAYSVYKSGSIFVSMLLH
                     FLNNSVAVVLNHYTTGNITNLYKFVEVDFSNLNVLKFALILLISLILIMLGLRVLDRK
                     SLRN"
BASE COUNT         1236 a          323 c          516 g         1129 t
ORIGIN      
        1 ataatgtaaa aaaatgttac aaaagatatt ataaacgaat aaaaaataat aatttgtcag
       61 ataaacttgt aaatttttat gaataatcca ttctaaaaca aaaaactgtg tataaaaaag
      121 gtttatatgt tatatttata taaatataac atatgaacga gaaaggagtg gtttgataaa
      181 tgattgaggt agataaattg tgtaagtctt ttacaagggt tgtaaaagat gataagaata
      241 aaagttcaat aagaaagcta aagaaagtaa aaactaaaaa agaagaattt tttgctgtaa
      301 ataatgtttc atttaaagtt atggaaggtg aaattgttgg aatattaggt ccaaatggtg
      361 caggaaagac tacattactt agaatgttag gtggcatatt aacaccaaca tcaggaagta
      421 taaatatttc tggatatgat tattcaatag atagaaattc agcaaaaaaa gaaataggtt
      481 atctctctgg aaatacaaaa ctatatggaa ggctttctcc gagagaactt ctaacaactt
      541 ttgcaagtct atatgagatg tcaaaagaag atatagaaga atcaattgaa aatattgtaa
      601 aaataatgga tatgagtgaa tttatagata atagaattga aaatctatca actggtcaga
      661 ctcaaagaac atctatagct cgttgtttaa tacattctcc aaaagtatat atttttgatg
      721 agccaacact aggtctggat gttttaagta gtgcatctat tatagatttt atgaagaatg
      781 aaaaactaag gggaaaaact gttctttact caactcatta tatggaagaa gcagaaactt
      841 tatgtgacaa gatttttatg attcacaatg ggaaaataat tgcatcagga acaccacaga
      901 gtttaaaaga ggaatctgga gtaaataatt tgagagatgt attcataaaa cttgccagaa
      961 aggaggacat ttaaatgaga agtaagattg taaaatacat atttaaaaaa gaaatgttag
     1021 acatattgag agataaaaag actttattta tgatgattgt tgtgccactt cttttatatc
     1081 ctttacttat gttattaatg attcaagtaa tgaatatgtc tgcaagtagt atgaatagta
     1141 aggacattaa tatagctttt aataataaac ctaataaggt gttagtatcc atgattgaag
     1201 atgataattt agaaaaagat actaaaaata cttcaaatga taaaggcaaa ataagagtgt
     1261 tagatgtaga taactataaa aagtcattag aagaaaataa aattgatgct tatataaaga
     1321 tggaagaaaa aaaatcatct ataaattatc aaatatatat aaattcttct caagaaaatt
     1381 cttctaatgc actaaaaaga attgaaactg ttttggataa atataaaaga tttaccatag
     1441 aaaaaacttt atctgaaaaa ggattagatg tacaagctac tttagaacca ataacatata
     1501 gcactgtaga tgttgcaaag acggaagaag tggcaggata ctttttaggt cagatacttc
     1561 catttatatt aataatagga gttttactag gttcaatata tccagcaata gatgttatgg
     1621 ctggagaaaa ggaaagaggt acattagaaa cattatttac actgccaata tcaaacttag
     1681 aactggttat gggaaaatat atggcagttt ctcttagtgc aatagtaaca gcaattttaa
     1741 acattttatc tatgggactg actatggcat ttattttagt tagtggaggt attagtagcc
     1801 aatttggctt tggaaacttt aattatggag acttagtctt accaattata actactttaa
     1861 tttgcatatg tcttttttca atggtagttt cagcaataag tatgtgtgta tgttcattag
     1921 caaaaagttt taaagatgct caaaactaca taacacctct tatgttaatt attatgatac
     1981 catcatatgt ttctatgata ccaaatatag tattagatag agtgacagct gtaatacctg
     2041 tagtaaatat atctttactt ataaaaagtg tattatcttt taaaagcgat ttaaatttaa
     2101 tagccttagt tttagcatca aatgtagcct ttgttatatt ggcaatcata ctactgtcta
     2161 agatgttcaa ttctgaggaa atactttttg gtaatagtaa gagtttttct ttcttagaga
     2221 aaagaagtaa tataaaaaaa gggtctatac caactgtaag tgatggtgtt attttatatg
     2281 cagtaggatt agttctattg atttatgttg gttctttagt tcaaattaaa tttggtatgg
     2341 caggtattat aatgacacaa ttcatgataa tttctttacc attattattt gcatactata
     2401 ttaaagctga ttttaaagag gtatttagct tgacagttcc taaattaaag catgtgtttg
     2461 ggagcattgt tctttggatt ggtggataca tacttattat gattataaca cagctaattt
     2521 tattcttatt tcctcaaaat atggaagttg ctgaaagttt aaataaggct ctttttatca
     2581 aagatagtgt tttactgaat ttactagcaa tagctttatt accagctata tgtgaagaaa
     2641 tgttttttag gggatttata ttttcttcat ttagtaaatc aaaagataaa aataagtcta
     2701 tcaaactcgc tataatatgt agtggggtac tatttggtat tatgcatatg gattttatta
     2761 gaataattcc aacatcaata ttaggtatta tatttgcata cagtgtgtat aaatctggtt
     2821 caatatttgt atcaatgtta ttgcattttt taaataatag tgtagcagtt gtgttaaatc
     2881 attatacaac tggaaatata accaacttat ataaatttgt tgaagttgat ttttcgaact
     2941 taaatgtgct taaatttgcc ttaattcttc taataagtct tatattaatt atgttaggat
     3001 taagagtttt agatagaaag tctttaagaa attaagtata aagttattat atagattaaa
     3061 atattaataa gcactaaagt atatggatat atcttgtata taaaaatact ttagtgctat
     3121 tttatatatt ttaattatat gaattattta tcgtttcatt tatgtaatga aaatgttata
     3181 taaatgaaac aataaataat tttt
//
LOCUS       DAMJVK010000200         3130 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.205, whole genome shotgun sequence.
ACCESSION   DAMJVK010000200 DAMJVK010000000
VERSION     DAMJVK010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.205"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            124..1566
                     /locus_tag="PC414_003770"
     CDS             124..1566
                     /locus_tag="PC414_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HDF2466115.1"
                     /translation="MRTIRRVLALGLTLAIFLINVPNVDALTSDTIKGNNIYETAGLI
                     ADKKSYDTAIMVNMDNSIADGLSASGLAGAVDAPILLAQKNKIPNETKQRLKNVKKIY
                     IIGKELSISKSVETELKNTGAQVTRLGGDDRIKTSYSVAKEVNGIKKVDEVILTNAYK
                     GEADTISAAPVSVRDIAPIVLTDGKSVPFSTSNVKTYAVGGSISMSTSLVNKTNAKRL
                     GGSDRYDTNKKVIKEFYPDASEFYLSDGYDLVNALTGSTIAKENPIVLVSESSDKSIL
                     AGADKITRLGSISDSVYNKCVSAAQNNGDSSTKGESPMKNETSILGQPTASLEACLKW
                     AKSKKANDLFIELIPILYDTAVQEGVNPVLAVAQSAKETGFCNFGGVLDASFKNPCGL
                     KTSVGGSDTDKNAHSRFDTWEEGILAQIQHLCLYAGQDGYPLSNPVDPRHEKSLFGKA
                     KTVESLSNNWAGGQYGQDLVRMMGEIEATK"
     gene            1608..1961
                     /locus_tag="PC414_003771"
     CDS             1608..1961
                     /locus_tag="PC414_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2466116.1"
                     /translation="MKKSNIFSIIFPTVIMVLMFLTFATDIFNIPDIQSKSIFVVGMV
                     LIFPISFLIQGIICVLNKTNWILSLIVSLVTYIVLMYIFLNDSAYIYVLLYTAFYMIG
                     HITAKLYYKIKTFKN"
     gene            2152..2973
                     /locus_tag="PC414_003772"
     CDS             2152..2973
                     /locus_tag="PC414_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HDF2466117.1"
                     /translation="MNLIDNHIHTNFSSDGKDSMEDTIKKAISIGVRYLTFTDHLEHD
                     EDRGFCINYNDYVPVFNKFKEKYKKDIELLLGVEVGYRKHLKNEIEEIINSNPFDFVL
                     CSTHTIDNVPVPSKAYFKGLSKEDAYYKYFNSILETNREFGDYNIYGHLDYISRYGIY
                     SDNRVIYNDFKDVIDEVLKSIINNGSGIELNTSGYRYGLNAIHPNEDILKRYKELGGF
                     IVTVGSDSHRVEDICKDFDVAYDMLKYLDFKYVSLFKERETYFVNIEKVKSNNIA"
BASE COUNT         1207 a          319 c          554 g         1050 t
ORIGIN      
        1 ttgtaattta tgatataata acaaatatat aattattaaa ttataataaa tgactatagc
       61 gtagatgtaa tactatgtta tagtcatttt tgaattaaac ttgatatatc aagggggaag
      121 aatatgagaa caattagaag agttttagca ttaggtttaa cattagctat atttctaata
      181 aatgtaccta atgtagatgc attaacatca gatacaatta aaggtaacaa tatatatgag
      241 acggctggat taatagcaga taaaaagagt tatgatacag caattatggt aaatatggat
      301 aattctatag cagatggtct ttcagcaagt ggtcttgcag gtgctgttga tgctccaatt
      361 ttgcttgcac aaaaaaataa gataccaaat gaaacaaaac aaagattaaa aaatgttaaa
      421 aagatataca taataggtaa agaattatca ataagtaagt cagtagaaac tgaactaaaa
      481 aacacagggg cacaggtaac tagattgggt ggagatgaca gaataaagac tagttatagt
      541 gttgctaaag aggtaaatgg tatcaaaaaa gttgatgaag tgatattaac taatgcatat
      601 aaaggagaag cagatacaat aagtgctgca cccgtatcag taagagatat agctcctata
      661 gtacttacag atggaaagag tgtgcctttt tcaacaagca atgtaaaaac atatgcagta
      721 ggtgggagta tatcaatgag cactagttta gttaataaaa ctaatgctaa aagacttggt
      781 ggttcagata gatatgatac aaataagaaa gttataaaag agttttatcc agatgcatca
      841 gaattttatt tgagtgatgg atatgattta gtaaatgcac ttacaggttc tacaattgct
      901 aaggaaaatc caattgtatt ggtgtcagaa agtagtgata agtctatatt agcaggagca
      961 gataagatta ctagattagg ttcaataagt gacagtgtgt ataataagtg tgtttctgct
     1021 gcacaaaata atggcgattc gtctacaaaa ggtgagtcac ctatgaaaaa tgagacaagc
     1081 atattaggtc agccaacagc tagtttagaa gcgtgcttaa aatgggcaaa atctaaaaaa
     1141 gctaatgatt tatttataga gttaatacca atattatatg atactgctgt tcaggaaggt
     1201 gttaatcctg ttttggcagt agctcaatct gcaaaagaaa ctggtttttg taattttggt
     1261 ggagtattag atgcatcatt taagaatcct tgtggactta aaacttctgt aggtggttct
     1321 gatactgata agaatgccca ttcaagattt gatacttggg aagaaggaat attagctcaa
     1381 attcaacatt tatgcttata tgcaggacaa gatggttacc cactttcaaa tccagtagac
     1441 cctagacatg aaaaatcttt atttggtaag gctaaaacag ttgaaagttt atctaataat
     1501 tgggctggag gtcaatatgg acaagattta gtaagaatga tgggagaaat tgaggcaaca
     1561 aaataattga agattatcaa atatccaata aataaggatt gattattatg aaaaagagta
     1621 atatttttag tatcatattt cctacagtca taatggtgct aatgttttta acttttgcta
     1681 cagatatatt taatattcca gatattcaat ctaaaagtat ttttgttgtg ggaatggttt
     1741 tgatttttcc aatttctttt ttgatacaag ggataatttg tgtattaaat aagactaatt
     1801 ggattttatc tcttattgtt tctttagtaa cttatatagt attaatgtat atatttttaa
     1861 atgattctgc atatatatat gttttacttt atacagcttt ttatatgatt ggacatataa
     1921 cagctaaact ctattacaaa attaagactt ttaaaaatta aattgaagta tataaaagta
     1981 acattaggag aagtatcctt aatgattttc taaaatcaga aataggtgct tctctttttt
     2041 gtaaaaataa aagtatgtaa ataaaattta accattataa ttttctttta acaaatgtat
     2101 acaataaact tgttgtataa ttaagataga taaaataaag ggagggataa tatgaattta
     2161 atagataatc atattcatac taatttttca agtgatggaa aggattccat ggaagataca
     2221 ataaaaaaag ctataagtat tggtgttaga tatttgactt ttacagacca tttagaacat
     2281 gatgaagata gaggattttg cataaattat aatgactatg ttccagtatt taataagttt
     2341 aaagaaaaat ataaaaagga catagagtta ttactaggtg ttgaagtggg ttacagaaaa
     2401 catctaaaaa atgaaataga agaaataata aattctaatc cttttgattt tgtattgtgc
     2461 tcaactcata caattgacaa tgtacctgtt ccatcaaagg cgtattttaa aggtcttagc
     2521 aaagaagatg cgtattataa atattttaat agcatacttg aaactaatcg tgaatttgga
     2581 gattacaata tatatggtca tttggattat atatctaggt atggaatata ttcagataat
     2641 agagttatat acaatgactt taaagatgtt attgatgagg tcttaaagtc aataatcaat
     2701 aatgggagtg gtatagagct aaatacttct ggttatagat atggactaaa tgctatacat
     2761 ccaaatgaag atattctaaa aagatataaa gaacttggag gatttatagt tactgtaggt
     2821 tctgattctc atagggtaga agatatatgc aaagattttg atgtggccta tgatatgctt
     2881 aagtatcttg actttaaata tgtatcttta tttaaagaaa gagaaaccta ttttgtaaat
     2941 atagaaaaag ttaaatctaa caatatagct tagagtgtgt tttgaagtaa attaatagat
     3001 agttaaaaag ttaatagtta tagtattgta tcctctagaa aaatactaga ggtttttatt
     3061 ttgctaaaat aactaaatag aattaaattt aaatagtata gcttatttta aattgaatag
     3121 tttattgttg
//
LOCUS       DAMJVK010000201         3078 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.206, whole genome shotgun sequence.
ACCESSION   DAMJVK010000201 DAMJVK010000000
VERSION     DAMJVK010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.206"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            <1..597
                     /locus_tag="PC414_003773"
     CDS             <1..597
                     /locus_tag="PC414_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003399531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2466118.1"
                     /translation="GLIKESSGQITLDSTTITNLAEYKRTQIVSRVFQDPSLGTCPSM
                     TVRENLSLALNKGKLLNLKKCLRHKTNDLEHLLEGISLDLKKYLDIKVQYLSGGQRQS
                     LSLIMSSLASPKVLLLDEHTAALDPKTSNEVIELTDKIVREKNITTLMVTHNLKHALQ
                     YGDRLIMLHKGEVVLDVKDKEKKDLTVEEILEKFEYAV"
     gene            694..1731
                     /locus_tag="PC414_003774"
     CDS             694..1731
                     /locus_tag="PC414_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2466119.1"
                     /translation="MIKSKKILSLIIAGVLGVSMLTGCSQNDGSNASNENKETDSKKQ
                     KNIGITQLVEHPSLDKAKKGFIKALEDKGYKDGDNIKIDFQNAQNDMPTTQSIASKFV
                     SDKKDLIYAISTPSAQAAYNATKDIPIIMTAVTDPVEAGLVKSLEKPGGNVSGTSDYL
                     SIDKTLELVKTLTPKAKKIGVIYNTSEVNSKIQVDSLHDYAKKNNYEVVEKGISSSSE
                     VNQAISSLVGKIDVLYVPTDNLIVSSMPIVSKVANENKIPIIASEEGSVSSGALACCG
                     IDYEKLGYKAGELAIEVLEGKSVGDIPVTTLDETEIIINEDTLKALDMQKLSADNIKY
                     IKSDENAKSTK"
     gene            2540..>3078
                     /locus_tag="PC414_003775"
     CDS             2540..>3078
                     /locus_tag="PC414_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2466120.1"
                     /translation="MSGIISVMTQSLILSIMALGVYITYKILDFPDMSADGSYTMGAS
                     IVAFSLTNGISPVVATLMAILCGCTAGLVTGILHIKFKISNLLSGILVMGMLYSINLR
                     IMGKSNIPLFSFKHLFNGEISPIVLALAFVFICKVLLDLFLKTGLGYTLKGVGDNSQM
                     IKSLGINIGSIKILGLMIS"
BASE COUNT         1157 a          403 c          467 g         1051 t
ORIGIN      
        1 gggcttataa aagaatcttc tggtcaaatt actttagata gtactactat aacaaattta
       61 gctgagtata aaagaactca aatagtaagt agagtttttc aagacccaag ccttggaact
      121 tgtccttcta tgacggttag agaaaattta tctctagcac ttaataaagg gaaattattg
      181 aacttaaaaa aatgtcttcg tcataaaacg aatgacttgg aacacttact tgaaggaatt
      241 tctttggatt taaaaaaata tttagatatt aaagtacaat atctttcagg gggtcaaaga
      301 caatcactat cactaattat gtctagctta gcaagtccta aggtcttatt actagacgaa
      361 catacagcag cacttgaccc aaaaacatca aatgaagtaa tagagttaac agataaaatc
      421 gtaagagaga aaaatataac tacacttatg gttactcaca acttgaaaca cgctcttcaa
      481 tatggagata ggttgataat gcttcataag ggtgaagttg tattagatgt aaaagataaa
      541 gaaaaaaaag atttaacagt tgaagaaatc ttagaaaaat ttgaatatgc agtataagaa
      601 attaaaatct tagttttaat cagaaatatt tataatctat catatgaata tatgaacaaa
      661 tataattatt aaattttaag ggaggaaaat aaaatgataa aaagtaaaaa aatattaagt
      721 ttaattatag caggagtact tggtgtatca atgcttactg gatgttctca aaatgatggc
      781 tccaatgctt caaatgaaaa taaagaaaca gatagcaaaa aacaaaaaaa tataggtatt
      841 actcaattag tggaacatcc atctctggat aaagcaaaga aaggattcat caaagcactt
      901 gaagataaag gctataaaga tggagataat ataaaaatag atttccaaaa tgcacaaaat
      961 gatatgccta ctacacaaag tattgctagt aagtttgtat ccgacaaaaa agatttgata
     1021 tatgctatat ctactccatc tgcacaagca gcttataatg ctactaaaga tattccaata
     1081 ataatgactg ctgttacaga ccctgtagaa gctggacttg ttaaatctct tgaaaaacca
     1141 ggtggaaatg tttctggtac atctgattat ctttcaattg ataaaacatt agaattggtt
     1201 aaaacattga ctccaaaagc aaagaaaata ggggttatat acaatactag tgaagttaat
     1261 tcaaaaatcc aagttgattc tctacatgat tatgctaaga aaaataatta tgaagtagtt
     1321 gaaaaaggaa tcagctcttc aagtgaagtt aaccaagcta tttctagttt agttggtaaa
     1381 atagatgttt tatatgtccc tactgacaat ttaatagttt cttctatgcc aatagtttct
     1441 aaagttgcta atgaaaacaa gattcctata atagcctctg aagaaggttc tgtatcttct
     1501 ggtgctttag cttgttgtgg aatagattat gaaaaactag gttataaggc tggagaactt
     1561 gctattgaag tattagaagg aaaatctgtt ggcgatatac cagttactac attagatgaa
     1621 actgaaataa taattaacga agatacacta aaagcacttg acatgcaaaa gttatcagca
     1681 gataatataa agtatataaa gtcagatgaa aatgcaaaat ctacaaaata aaacttatta
     1741 atttcaaaaa ataggtaata tgaagtaggt aatatgaaaa ctttatataa taagtactct
     1801 tatccaccct aaccctttga aaattgtgct tattgtagca atttgaatat tgcatataaa
     1861 gttattcatt ttccataaaa gtcgtctata ttgacgactg attatgaaag ttatataact
     1921 ttctaatcac tacttgagct gtccaaaaga attttaatat tctccagaca gctttttttt
     1981 atgatatttg attgatatac atgtctagtt attccatatt tagtaaatat tttgataaaa
     2041 tttccacatt cacttataac ttaggataac gtttttttca aacaatacat taatttaaaa
     2101 gtattctgag tatacattat ttagtatttt aattaaattt agcagttgac ttttattatt
     2161 gtataattta taatttataa caagtcgaga atacatataa ttttctgact aattaaaata
     2221 ttaaaaggga gagataatta tgaatacttt attaaacaat agaaaatatt attattatta
     2281 tagccactat ttttagggtt tgtacctacg ttttaacata gatacagacc ccttaagact
     2341 actaaaattt tgggattgta tctatgttgg ctatattgtt tataaagttt tcatctaact
     2401 atataagagc cacatagatt aaactatgtg gcttattcgt gtttttacca catagttttg
     2461 tacactatgt ggttttttta tacacaaaat tagcaaataa ttaaaattat aaaaatattt
     2521 tcaaaggagt gttttattta tgtcaggtat tatatctgta atgacgcaaa gtttaatttt
     2581 atcgattatg gcactggggg tttatataac ttacaaaatc ttagattttc cagatatgtc
     2641 tgctgatggt agctacacaa tgggtgcttc tattgtagca ttttctctta ctaatggtat
     2701 ttctcctgta gtagcaactt taatggctat cttatgtggt tgtactgctg gtcttgttac
     2761 agggattctt cacattaagt ttaagatatc taacttactt tctggtattt tagttatggg
     2821 aatgctatac tctatcaatt taagaataat gggaaaatca aatattcctc tatttagctt
     2881 taaacactta ttcaatgggg aaatatctcc tatagtttta gccttagctt ttgtattcat
     2941 ctgtaaagtc cttttagatt tattcctaaa aacaggtctt ggatacactt taaaaggtgt
     3001 tggtgataac tctcaaatga ttaaatccct aggtataaat atcggttcta taaaaatact
     3061 aggacttatg atttcaaa
//
LOCUS       DAMJVK010000202         3025 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.207, whole genome shotgun sequence.
ACCESSION   DAMJVK010000202 DAMJVK010000000
VERSION     DAMJVK010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.207"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            386..1663
                     /gene="hflX"
                     /locus_tag="PC414_003776"
     CDS             386..1663
                     /gene="hflX"
                     /locus_tag="PC414_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDF2466121.1"
                     /translation="MDMAKKGITVGLNINNKSEDFNELMIELENLCSACDIDVVGSIT
                     QNAKQVNRAFYIGTGKVEEILNLIKKENIEIVIFYNELSTSQLKNLEEKLNCEIIDRT
                     ALILDIFAQRAKTREAKLQVEVASLKYMLPRLIGSNENLGRQSGGVGTKNRGSGEKKL
                     ELDRRRIEEKITSLNRELDDLKFQRETQRSMRRKSNLPNVALVGYTNAGKSSIMNKLV
                     DIFKNSEEKKVFEKNMLFATLETSVRNIVLANNKEFLLSDTVGFVSNLPHDLVKAFRS
                     TLEEACEADVLLHVIDISNPSYKSHIKVTEDTLKQIGADGIPMIHVYNKIDLIDVEVL
                     DRILDSIDKEGIFVSVKKDINIDKMIKCICDSIFKDYVRCKFLIPYDKGHVVSYFNEN
                     TSIINTEYREDGAILDVECSNIEYNKYKKYALE"
     gene            1896..2393
                     /locus_tag="PC414_003777"
     CDS             1896..2393
                     /locus_tag="PC414_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2466122.1"
                     /translation="MDAESLMKKYMVDDDSRKQAIFASIFVMQNKLQTTCDKLDPELT
                     MKQWLLLAIADSVDETLTLTKLGELMGCSRQNVKKLAIALEKKGFIQIEQNEKDSRAI
                     CLVTKDRVQEYSKKVRGMQEKVLQLLFEDFTEKEVEQLFFGIMKLSIGIENVKNYVEN
                     NDVGE"
     gene            2443..2967
                     /locus_tag="PC414_003778"
     CDS             2443..2967
                     /locus_tag="PC414_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDF2466123.1"
                     /translation="MKNVVVIYKSKYGSTKKYAKWLSELLSCDIFETKNINIEKVMKY
                     DTIILGGGLYACNIAGISFLKKNYKELQKKNLVVFGVGAAPYDDEVIKKLREVNFKDE
                     LNKIPCFYCRGAWDETKMSFIDYTLCSFFKKSIAKKERQGLKPAEAPLLSGEAFDWCN
                     KENLTPILDYIREL"
BASE COUNT         1253 a          267 c          533 g          972 t
ORIGIN      
        1 tttattaatc atgcaaataa ctacttaaat ttaagaatag ggttaaatat aaaatgataa
       61 ttacatataa gttaattaaa ctaatgtatt tttaatatat atttagaatt ttaatccttc
      121 ttatgatata agtagcttgt tttgtattac ttagatttgt aaggagtttt tttattgaaa
      181 aaactgcata atatatttgg gaggaggtgt aagaaagtga ttttttatat aagattgaga
      241 aaataatgca cgattatggt tattataata gtaaataaga agtgtcattt aaggataaga
      301 taaaaagcaa aattcttgat gaatttaatt atagagccat aatctcgtgc tatagattaa
      361 ataaaaaata gcagataagg agaatatgga tatggcgaaa aaaggaataa cagtaggatt
      421 aaacataaat aataaatctg aggactttaa tgaactaatg attgaacttg aaaacttatg
      481 ttcagcatgt gatattgatg ttgtagggag tataactcaa aatgcaaagc aggttaatag
      541 agcattttac attgggacag gtaaagttga ggaaatatta aacttaataa agaaagaaaa
      601 tatagagata gttatatttt acaatgaact ttctacatca caactaaaaa atcttgaaga
      661 aaagcttaat tgtgaaatta tagatagaac ggcattgata cttgatatat ttgcacaaag
      721 ggcaaaaact agagaagcta aacttcaagt agaagtagca agtttaaaat atatgttacc
      781 aagacttata ggttcaaatg aaaatttagg acgtcaaagt ggtggagttg gtactaaaaa
      841 tagaggttct ggtgagaaaa aattagaact tgaccgtaga agaatagaag aaaagataac
      901 atctttaaat agggagttgg atgatttaaa attccaaaga gaaactcaaa gaagtatgag
      961 aagaaaatca aatcttccaa atgtagctct agtcgggtat acaaatgctg gtaaatcttc
     1021 aataatgaat aaactagtag atatatttaa aaactctgaa gaaaagaaag tgtttgagaa
     1081 aaacatgtta tttgctacac ttgaaacttc tgtcagaaat atagttcttg caaataacaa
     1141 agaattttta ttatctgata ctgttggttt tgtaagtaat ctaccacatg acttagtaaa
     1201 agcatttagg tcaacattag aagaagcatg tgaggcagac gtactgttac atgttattga
     1261 tatttcaaat ccaagctata aaagtcatat taaagtgaca gaagatactt taaaacaaat
     1321 aggtgcagat ggtataccta tgatacatgt gtataataaa attgatttaa tagatgtaga
     1381 agtattggat agaattttag atagtatcga taaagaaggt atatttgttt cagttaaaaa
     1441 agatataaat atagataaaa tgataaaatg tatttgtgat agcatattta aagattatgt
     1501 aagatgtaag ttcttaattc catatgataa aggtcatgta gtttcttatt ttaacgaaaa
     1561 tacgagcatt ataaatacag aatacagaga agatggagct attttagatg tagaatgtag
     1621 caatatagaa tataataagt ataaaaagta tgctttagag taaattttga aaaacttatt
     1681 atagcctaaa atatatttaa tttataattt attataaatt gattgtaaat atgattttat
     1741 gaaacggtat agccataatg ctatgccgtt ttgccgttta taataaaaat aaatttattg
     1801 acataatagt aactaattac tataatataa agtaattagt tactatttaa aattatcaaa
     1861 aaattaaaat ctaagataaa gagaagtgta taataatgga tgcagaaagt ttaatgaaaa
     1921 aatatatggt agatgatgat agtagaaaac aagctatatt tgcaagtata tttgtaatgc
     1981 aaaataaact tcaaactaca tgtgataaat tagacccaga attaacaatg aaacaatggt
     2041 tacttttagc tattgcggac tctgtagatg aaactcttac attaaccaaa cttggagaat
     2101 taatggggtg ctcaagacaa aatgtaaaaa aactagctat tgctttagaa aagaaaggtt
     2161 ttatacaaat tgagcagaat gaaaaagatt caagagctat ctgtcttgtg acaaaagata
     2221 gagtgcaaga gtattctaaa aaagtaagag gtatgcagga aaaagtatta cagcttttat
     2281 ttgaagattt tacagagaaa gaagtggaac agttattttt tggaattatg aaattaagta
     2341 taggaattga aaatgtgaaa aattatgtag aaaataatga cgttggagaa taagcaagta
     2401 tcaaatcaac tttgtatgac atcagagagg gggataattt atatgaaaaa tgtagtagtg
     2461 atttataaga gtaagtatgg ttcaacaaaa aaatatgcta aatggctttc agaattattg
     2521 tcttgtgata tttttgaaac taagaatatt aatatagaaa aggttatgaa gtatgatacg
     2581 attattttag gaggaggtct ttatgcttgt aatatagcgg gaatttcttt cttaaaaaaa
     2641 aattataaag aattgcagaa aaaaaattta gtagtatttg gcgtaggagc tgctccatat
     2701 gatgatgaag taataaagaa attaagagaa gtaaatttta aggatgaatt aaacaaaatt
     2761 ccttgctttt attgtcgtgg ggcatgggat gagacaaaaa tgtcttttat agattatact
     2821 ctttgtagtt tttttaaaaa atctatagct aaaaaagaac gtcaaggtct taaaccagca
     2881 gaagcacctt tattgtctgg agaagccttt gattggtgta ataaagaaaa tttaactcca
     2941 atacttgatt atataagaga actttaagag gtatatattt gtaattattt attatctaaa
     3001 gttagatttc atagaaactt actag
//
LOCUS       DAMJVK010000203         3021 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.208, whole genome shotgun sequence.
ACCESSION   DAMJVK010000203 DAMJVK010000000
VERSION     DAMJVK010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3021
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.208"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(55..1002)
                     /locus_tag="PC414_003779"
     CDS             complement(55..1002)
                     /locus_tag="PC414_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2466124.1"
                     /translation="MRITKTTFNDVFSNDANGSIIKVEKYVDKKCASVFEILTYTIIV
                     TNTSRYKTGNIFFKDYISKYIEFINNTVKVNGIIKRGLDPQQGFYIGRIDASCKKIIS
                     FKSVVLPNSAHRIIKNSANIYYYYKCNLDKFPTRISIESNRVYTNVNKIVFKQLNISN
                     TLKAPKNLRDILKVNVNSKIFDVKPMKNLINKGSNLKLCNLIVFGYIEVEIDYSCKNR
                     SNFKTVNKSNDIKKDKYYKNDIQNNNENINDCKKQISFGDNKVKKITKTFGFSCFICS
                     PVGIIYEDMKNINIKIEHTSINELNPEELFVNTSLLLYY"
     gene            complement(1030..1521)
                     /locus_tag="PC414_003780"
     CDS             complement(1030..1521)
                     /locus_tag="PC414_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2466125.1"
                     /translation="MYENNKVIYNLERCEKLEKTLYLSQFNTYKNFKLASKKTVINSL
                     ETYVYNMNINNISLVDTIECISLEDEQLTGKKLILVGDIDIDAILDYAGNKRNRNSSK
                     KNTFKLKIPFSTFIQMPRKIENKDKINLKYLIQDITSSILDDNLFISVTAIISYENSS
                     KIE"
     gene            complement(1964..2857)
                     /locus_tag="PC414_003781"
     CDS             complement(1964..2857)
                     /locus_tag="PC414_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDF2466126.1"
                     /translation="MKNKKFIVIILIISILIGVLVKEYSSREIKKDDINVSKYIKYAD
                     LASKNNAQVNWKYVASIVAVLNKNNLKNVKDSQIQEVSDLFVKNFSKNNKINKLSDIL
                     DELEFSNRQKRLVDNYIDNLKDYGIKPERLKSDTKYMKFIDEIKTEAIQNYKDYKILP
                     SITIAQAIIESSWGKSTLAKQYNNLFGIKADAYWKGKSVTLETKEHLDTIIDDKFRIY
                     DDKNESIKDHAKFLATNKRYKNNGVFDAKTYIYQAKALEKAGYSTAKDENGNSIYAAR
                     LIELIQQYNLQLIDSEIQSEM"
BASE COUNT         1009 a          427 c          267 g         1318 t
ORIGIN      
        1 ttttccttta accaaatgct attttaataa gtttaagttt tagctttaaa atacttaata
       61 atacaataaa agtgatgtat ttacaaataa ttcttctgga tttaattcat ttattgatgt
      121 atgttcaatt tttatattta tatttttcat atcttcataa ataattccaa ctggagaaca
      181 aatgaaacaa gaaaatccaa aagtttttgt tatttttttt accttattat caccaaaact
      241 tatttgcttt ttacaatcgt ttatattttc attgttattt tgtatatcat ttttataata
      301 cttatctttt ttaatgtcat tagacttatt tactgtttta aaattactcc tatttttaca
      361 actatagtct atttcaactt ctatataccc aaaaactatc aagttacata atttcaaatt
      421 actacctttg tttataagat ttttcatagg tttaacatca aatattttac tatttacatt
      481 tacttttaat atatctctta gatttttagg agcttttaaa gtattactta tattcaattg
      541 tttgaataca attttattta cattagtata aactctatta ctttctattg atattcttgt
      601 tgggaattta tctagattac acttataata atagtatata ttagcactat tctttataat
      661 cctatgtgca gaatttggta aaactacact cttaaaagat attatttttt tacaacttgc
      721 atctattctc cctatgtaaa aaccttgctg tgggtctaat ccacgtttta taatgccatt
      781 aacttttacc gtattattta taaactctat atatttagaa atataatctt tgaaaaatat
      841 atttccagtt ttgtacctac ttgtatttgt aacaataata gtgtaggtca aaatctcaaa
      901 tacagatgca cattttttat caacatattt ttctactttt ataatacttc catttgcatc
      961 atttgaaaat acatcattaa aagtagtttt cgttattctc attgcatcca cctttctata
     1021 atttaaagat tactctatct tggaactatt ttcataactt ataattgcag taacactaat
     1081 aaataaatta tcatctaaaa tagatgaagt aatatcttgt ataagatact ttaaattaat
     1141 tttatcttta ttttctatct ttctaggcat ttgtataaat gtactaaagg gtatttttaa
     1201 tttaaatgta ttttttttac tagaatttct atttctttta ttgcctgcat aatctaatat
     1261 tgcatcaata tctatatcac caactaaaat caacttcttt cctgtaagtt gttcatcttc
     1321 taatgatata cattctatag tatcaacaag acttatattg ttaatattca tattgtaaac
     1381 atatgtctct aatgaattta ttacagtttt tttagaagct aatttaaaat ttttatatgt
     1441 attaaattga gataaataaa gagtcttttc tagtttttca catctttcaa ggttgtatat
     1501 taccttatta ttttcataca taatcgcatc aatctccctg tatctatata ttaatactat
     1561 tcataaaaat tatttcaaat taccattttt tattattcac tatctagttg ttatttactt
     1621 ttaagaaaga atcctttttt acttcattga tttcaattta caagtaaaag tctttctgaa
     1681 aaaaaaatat atatatctct attcactttt ttataagtaa ttataatttt tatagactaa
     1741 cggtcagcct taagggaaaa ggtgttcttt aagtgcagaa tttagataaa gtaaataatc
     1801 cttttataaa taattccaag ttttaactta aataatttga tttacagtat aaagtaaatt
     1861 ttgaaagata atgatatcga tttaataatt acaagtaaaa gcatgtcaaa ataaggctat
     1921 gattttaaaa atcatagcct tatcaataaa aatttaatca aagttacatt tcactttgaa
     1981 tttcactgtc tattaattga agattatact gttgaattaa ttctattaga cgagctgcat
     2041 atatactatt gccattttca tccttcgcag tactataacc tgccttttct aatgctttag
     2101 cttgatatat atacgttttt gcatcaaata caccattgtt tttatacctt ttatttgtgg
     2161 ctaaaaattt tgcatggtct tttatagatt catttttatc atcatatatt ctaaatttat
     2221 catctataat tgtatctaaa tgttctttcg tttctaaagt cactgacttg cctttccaat
     2281 aagcgtcagc ctttatacca aatagattgt tatattgttt tgccaaagtt gacttgcccc
     2341 agctagattc tattatagct tgtgcaatag ttatagatgg aagtatttta taatccttat
     2401 aattttgtat tgcttctgtt ttaatttcat caataaactt catatatttt gtatcagact
     2461 tcaatctttc tggttttata ccataatcct taagattgtc tatataatta tcaaccaatc
     2521 ttttttgtct attactaaat tctaactcat ccaaaatatc acttaactta tttattttat
     2581 tatttttaga aaaattctta acaaataaat ctgatacttc ttgtatctgt gaatccttaa
     2641 cgttttttaa gttattttta tttaacacag caacaatgga agctacatac ttccagttaa
     2701 cttgtgcatt atttttactt gctaaatcag catattttat atacttactg acatttatat
     2761 catccttttt tatctctcta gatgaatatt ccttcactaa tacacctatt aaaattgata
     2821 ttatcagtat tataacaatg aattttttat tcttcataaa tattgtcctc ccttatacta
     2881 tctgctgaat ataatatacc ttacttttgt gacatattct ttacgaaatt ttgaaaattt
     2941 tatgaagatt tatgaaatat atttaattaa aagcactact attgttataa tatatagttt
     3001 tctatcacac aaaatagtta a
//
LOCUS       DAMJVK010000204         3008 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.209, whole genome shotgun sequence.
ACCESSION   DAMJVK010000204 DAMJVK010000000
VERSION     DAMJVK010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.209"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            135..503
                     /locus_tag="PC414_003782"
     CDS             135..503
                     /locus_tag="PC414_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF2466127.1"
                     /translation="MENLVKIFKALSDETRLNILILVSKRNICQKGISKYLGISDSAV
                     SQHIKILKDVGIITGYKEGYYVLYHINKESFNICVDFINSMLSNSSESFIDVFDVNTI
                     HLGCSKECKSIKKCCKRREK"
     gene            506..877
                     /locus_tag="PC414_003783"
     CDS             506..877
                     /locus_tag="PC414_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HDF2466128.1"
                     /translation="MKVCIPVEENKGLDSKPYGHFGSAPIFVVCDLESGEVKSLDNGD
                     LDHEHGKCQPLKALSGTAVDAVVVGGIGQGAIIKLNGMGIKVYRAEGDTISANLDLLK
                     NGKLVEFPSDHTCSHDGCGHH"
     gene            complement(1022..1477)
                     /locus_tag="PC414_003784"
     CDS             complement(1022..1477)
                     /locus_tag="PC414_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2466129.1"
                     /translation="MNINTVQGDSIEVLLRQLGATRISKVSSTLYFIKFDLGDGWEIS
                     YTYNINAKDQYFLQRIEPYPIGRGLFNDEYEIVSFISKDLKKFLNAKNSSNFKTFVEV
                     TRKVNSIIDNVEELFLNYNVDGDDLKTLNKELNDILNDIDYIEKHTKKI"
     gene            complement(1516..2781)
                     /locus_tag="PC414_003785"
     CDS             complement(1516..2781)
                     /locus_tag="PC414_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HDF2466130.1"
                     /translation="MEFQQIIAICIFLIVMAAIITEKVNRSVAAVGGALLMIIFNILT
                     LDEGLSHIDFNTIGVLVGMMLFVAVVKNSGLFEYIAIWTAKKAKGDPWKIMICFAIIT
                     AILSAVLDNVTTVLLIGPMTIVITQILGLNPVPFLITQILASNIGGTATLIGDPPNIM
                     IGSAANLSFMDFVINLGPAVIVILAITIICFRFIYGKELVVNERAKNAILKLDEKKSV
                     KDKPLLIKSLILIAFILFGFMFHSTIHIDSSVVALTGASIMLLIGKQDVDEIMAGIEW
                     STILFFMGLFVVVGGLVEVGIINKLAQALIGLTEGHLVFTMLLILWLSAIVSSFLDNI
                     PFVATLIPLILTMQAEGIDVMPLWWATSLGACLGGNGTLIGASANVVLAGIGNKHGHP
                     ISFKEYFKIGFPLMIISIIISTVYLIIKF"
BASE COUNT         1123 a          416 c          411 g         1058 t
ORIGIN      
        1 taaaactcaa agttatatta gtaaaaattg gaattattta taatatactc caaaatttaa
       61 aaataatggt tgaaaaatct aattaagagt actataattt aattaagtaa ttacttaatc
      121 aaaatgaggt acttatggaa aacttggtta aaatatttaa agcattgagt gatgaaacta
      181 gacttaacat acttatatta gtatccaaga gaaatatatg tcaaaagggg atttctaaat
      241 atctagggat ttcagattct gctgtttcac aacatataaa aatattaaaa gatgttggga
      301 taataacagg atataaagaa ggatactatg ttttatatca tattaataaa gaatcattta
      361 atatatgtgt agattttata aattctatgt taagtaattc tagtgaaagc tttatagatg
      421 tatttgatgt aaatacaata catctaggat gcagtaaaga atgtaaatct ataaaaaaat
      481 gttgtaagag aagggagaaa taataatgaa agtatgtata ccagtagagg agaataaagg
      541 attggatagt aaaccatatg gacattttgg ttctgctcca atttttgttg tgtgtgattt
      601 agaaagtgga gaagttaaat ctttagataa tggagattta gatcatgaac atggaaaatg
      661 tcaaccatta aaagcattgt cagggacagc tgtagatgca gtagttgttg gaggaatagg
      721 acaaggagct ataattaaat taaatggaat gggaataaaa gtttacagag cagaaggtga
      781 tactataagt gctaatttag acttacttaa aaatggaaaa ttagtagaat ttccatcaga
      841 ccatacttgc tcacatgacg gatgtggaca tcattaaaaa aaaacagctg atgaaatata
      901 tacttcatca gctatttttt cttttaataa atacgatttt attcttttaa aaccaaaatt
      961 tagattttta tgttttttgt agtatcctta ataatatttc aagtttacaa gtttactact
     1021 tttagatttt ttttgtatgt ttttctatat agtcaatatc atttaatatg tcattgagtt
     1081 ctttattaag tgtttttaaa tcatcaccat ctacattgta gtttaaaaat agttcctcta
     1141 cattatcaat gatagaattg acttttctag taacttcaac aaatgtctta aagttactac
     1201 tatttttagc atttagaaat ttttttaaat cctttgatat aaatgatact atttcatatt
     1261 catcattaaa tagacctcta ccaataggat atggttcaat tctctgtaaa aaatattggt
     1321 ctttagcatt aatattataa gtataagata tttcccaacc gtctcctaaa tcaaacttga
     1381 taaaatataa tgtactactt acttttgata ttcttgtggc accaagctgt cttaaaagaa
     1441 cttcaatcga atcaccttga acagtattaa tattcatata tacacatccc ctttagtatt
     1501 attatactat attttttaaa attttataat taaataaaca gtagatataa taatagatat
     1561 aatcataagt ggaaaaccta ttttgaaata ctccttaaaa gagataggat gaccgtgttt
     1621 attacctata cctgctagta caacattagc agatgctcct attagtgttc catttcctcc
     1681 aagacaagct ccaagagatg ttgcccacca tagtggcatt acatcaatac cttcagcttg
     1741 catagtcaaa ataagaggaa ttagtgttgc aacaaatgga atattatcta agaaagaaga
     1801 gactatagct gacaaccata atatcaacaa cattgtaaat acaagatgtc cttctgtaag
     1861 tccaatcaat gcttgagcca atttgtttat tatcccaact tctactaatc caccaactac
     1921 tacaaataat cccataaaga ataaaattgt agaccattca atacctgcca taatttcatc
     1981 tacatcttgt tttcctatta aaagcattat agatgctcct gtcagtgcta caacagaaga
     2041 atctatgtgg attgtactgt gaaacataaa accaaaaagt atgaatgcta ttaatataag
     2101 gctttttata agtagaggtt tatctttaac agattttttt tcatctagtt ttaatattgc
     2161 atttttagct ctttcattca caacaagttc ttttccataa ataaatctaa agcatattat
     2221 agttatagca agtattacta taacagcagg acctaaattt attacaaaat ccataaaact
     2281 aagattggca gcactaccaa tcataatatt tggagggtct cctataagag tagctgttcc
     2341 tcctatattt gaagctaaaa tctgtgttat taaaaatgga actggattaa gtcctaatat
     2401 ttgtgtaata actatagtca ttggtccaat aagtaagacg gttgtaacat tatctaatac
     2461 agctgataaa atagctgtta taatagcaaa acaaatcatt atcttccatg ggtcaccttt
     2521 ggctttcttt gcagtccaga tagctatata ttcaaataac cccgagtttt tgacaacagc
     2581 tacaaatagc atcataccaa ctaatacacc aatagtattg aaatctatat ggcttaatcc
     2641 ttcatcaagt gtaagtatat tgaatattat cattaatagt gcaccaccaa cagctgctac
     2701 ggatctgttt accttttctg taataatagc tgccataact attaaaaata tacatatagc
     2761 tattatttgt tgaaattcca taatataagt acctccattt gtattatttt gtgcctaatg
     2821 ctagtactat atatagtatt acttttttat tgttttttca atggatgtat gaaagtatat
     2881 tatttaacaa agtttttgga aaattgacag aatttaaaga aaatttttgt tatttaaata
     2941 aattttaaaa ttattccatt gataataata ttaaacgaca aacgaaaaaa tatacccttt
     3001 aaactaga
//
LOCUS       DAMJVK010000205         2999 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.210, whole genome shotgun sequence.
ACCESSION   DAMJVK010000205 DAMJVK010000000
VERSION     DAMJVK010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.210"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            70..2427
                     /locus_tag="PC414_003786"
     CDS             70..2427
                     /locus_tag="PC414_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HDF2466131.1"
                     /translation="MDKVNLERYSCDINVGLNKNQLQSRIDDNLINAFDNGKTKTYKQ
                     IFKDNIFTLFNLINIVLVCLLVSVGSFKNTLFIFIAVINTIIGVIQEIRAKRLLDNLS
                     VIVSNKVSVIREKEKKEIDVQELVLDDIMILKTGNQICADSKVLNGKLEVNESLVTGE
                     SDIIIKNKGDFLYSGSFVVSGEAFVRVENIGKDNYANKIANDAKISKKHNSQLRNSLN
                     AILKIVGIIIIPLGIILFAKQHFGNGDSIATSVVGTVAAVNGMIPEGLTLLTSIALAV
                     GTIKLASHKTLVQELYCVETLARVDTLCLDKTGTITEGKMQVDDIVMLEEVDINEIMG
                     NICNSLKDDNATLNAIRDKYNVLDTYKAKNLIPFSSERKYSGVTFENKGTYLLGAFEF
                     IFKEKNKKLRIEVERYSKKGNRVIVLAHSDSYMEDNNIPEDIKPLAFILILDKIRDEA
                     KETLEFFYNEGVDIKIISGDNPITVSEVARKAGLKTASNFIDATTLKNDSDIYKAVDK
                     YSVFGRVSPQQKKQMILALKKQNHTVAMTGDGVNDVLALKEADCSIAMASGSDVTKNV
                     SNLVLLDSNFASMPKVVMEGRKVINNIQRASSLFLVKTIFSFFLSLLTLFFFSRQYPF
                     VPIQLSLIGAVTVGIPSFFLALEANKSRISGNFLLNILKNALPGALCVTVNVIIVYNI
                     VEYLGYSSSVFSTMCAILTGVCGLLILRQQCLPFNKERLFLIVSMTIAFVIGILFLGG
                     LFSFVALSRELILITMALAILMPIMIKVMLFVLDEILETIVPDLN"
     gene            complement(2513..2830)
                     /locus_tag="PC414_003787"
     CDS             complement(2513..2830)
                     /locus_tag="PC414_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HDF2466132.1"
                     /translation="MSEFKNVTAVKKANVYFDGKVSSRVIILPNGERKTLGLMLPGEY
                     TFSTREEEIMEMLAGSMDVKLPGSNEFVTYKEGQKFNVPSDSSFDLKVNEVVDYCCSY
                     IAD"
BASE COUNT         1145 a          330 c          486 g         1038 t
ORIGIN      
        1 aaattgagaa ttttataata tatttgtaga tttgagtata aaaaagtgct aagttgaaag
       61 gagtaaaata tggataaggt taatttagaa aggtatagtt gtgatataaa tgttggttta
      121 aataaaaatc aacttcaaag tagaatagat gataatttaa ttaatgcatt tgataatgga
      181 aaaaccaaaa catataaaca aatttttaaa gacaatatat ttacactttt taatctcata
      241 aatattgttt tagtctgttt attagtttca gttggttctt ttaaaaatac cttatttata
      301 tttattgctg taattaatac tattatagga gtaatacaag agataagagc taaaagatta
      361 ttggacaatc tatctgttat agtttcaaat aaggtatctg tcataagaga aaaagagaaa
      421 aaagagattg atgtacaaga attagtatta gatgatatta tgattttaaa aacaggtaat
      481 caaatttgtg cagattcaaa agttttaaat ggaaaattag aggtaaatga gtctttggtt
      541 acaggtgaat ctgatattat tataaaaaat aaaggcgatt ttttatattc tggaagtttt
      601 gtagtttctg gagaagcttt tgtaagagtt gaaaatatag gaaaagataa ctatgcaaat
      661 aagatagcta atgatgcaaa gatttctaaa aaacataatt ctcaacttag aaattcatta
      721 aatgcaatat taaaaattgt aggtataata ataattcctt taggaattat attgtttgca
      781 aaacaacatt ttggaaatgg agattctata gctacatctg tagtaggtac agtggcagct
      841 gtaaatggta tgataccaga gggattaaca ttacttacga gcattgctct agctgtagga
      901 actataaaac ttgctagtca taaaacttta gtgcaagaac tgtactgtgt agagactctt
      961 gctagagttg atacactatg tcttgataag acaggtacaa ttacagaagg taaaatgcaa
     1021 gttgatgata tagtaatgtt ggaagaagtg gatattaatg aaataatggg gaatatatgt
     1081 aattctctca aagatgataa tgcaacacta aatgcaataa gagataagta taatgtttta
     1141 gatacgtata aagctaagaa tttaatacca ttttcttcag aaagaaaata cagtggggtt
     1201 acctttgaaa ataaaggtac atatttatta ggtgcatttg aatttatatt taaggaaaaa
     1261 aataaaaaac ttaggataga agttgaaaga tattctaaaa aaggaaacag agtaatagtt
     1321 ttagctcata gtgattctta tatggaagat aataatattc ctgaagatat aaagccacta
     1381 gcttttatat tgatattgga taaaattaga gatgaagcta aggaaacttt agaatttttt
     1441 tacaatgaag gtgtagatat aaaaattata tctggtgaca atcctataac agtaagtgag
     1501 gtagctagaa aagcaggtct taaaacagct agtaatttta ttgatgcaac aacacttaaa
     1561 aatgatagtg atatatacaa agcagttgat aaatacagtg tttttggaag ggtaagccca
     1621 caacagaaaa aacaaatgat tttagcatta aaaaaacaaa atcatacagt agctatgact
     1681 ggagatggtg taaatgatgt gctagcactt aaagaagcgg attgtagcat tgccatggca
     1741 tctggaagtg atgttactaa gaatgtgtct aatttagtct tattagattc aaactttgca
     1801 tcaatgccaa aggttgttat ggagggaaga aaagtaatta ataacattca aagagcatct
     1861 tctttgtttt tagtaaagac aattttttca ttcttcttgt cgttattaac attattcttc
     1921 ttcagtagac agtatccatt tgttcctata caattatcac tcattggggc tgtaacagtg
     1981 ggtataccat cattcttctt agctcttgaa gcaaacaaaa gtagaatttc aggaaatttt
     2041 ttactcaata tattaaaaaa tgctttacca ggagcactat gtgtgacagt aaatgtaatt
     2101 attgtttaca atatagttga atatttaggt tatagttcaa gtgtattttc cacaatgtgt
     2161 gctatattaa ctggtgtatg tggtctttta atattaagac aacaatgttt gccatttaat
     2221 aaagaaagat tatttttaat agtaagtatg actattgcat ttgttattgg gattctattt
     2281 ttaggtggat tattctcatt tgtagcattg agtagagaat taattctcat aactatggca
     2341 ttagcaatat taatgccaat tatgataaaa gttatgttat ttgtcttaga tgagatatta
     2401 gagacaattg ttccagattt aaattaattt taaaatgata taaataagag cagttttagt
     2461 tgaactgctc tttttagatg tgtttaattt tatgtaagaa tatgtatttt tattaatcag
     2521 ctatatatga gcaacagtag tcaacaactt cattaacctt taagtcaaaa cttgaatcac
     2581 taggaacatt aaatttttgc ccttctttat aagttacaaa ctcattgctt ccaggcagtt
     2641 ttacatccat acttccagca agcatctcca ttatttcttc ttctctagta gaaaaagtgt
     2701 attctcctgg caacattaat cctaaagttt ttctttctcc attaggcagt attattactc
     2761 tgctacttac cttaccatca aaataaacgt ttgccttctt tactgcagta acattcttaa
     2821 attcactcat tagataaaac ctccaaaatt atttgtacat atatattata taaaaataat
     2881 gtaaaaaatg ctatatattt acaaaaaaat ttagtattta ttaaaaaaat taataagaat
     2941 aaaaaagaga tgaaattgct taaattgata aaaaatataa aataaattgc aaagtaagc
//
LOCUS       DAMJVK010000206         2894 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.211, whole genome shotgun sequence.
ACCESSION   DAMJVK010000206 DAMJVK010000000
VERSION     DAMJVK010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.211"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     regulatory      3..96
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            179..664
                     /locus_tag="PC414_003788"
     CDS             179..664
                     /locus_tag="PC414_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDF2466133.1"
                     /translation="MISELMLKNFRKSSINFKTKIKLNLREKPDINSLKLKSIPEGKI
                     VKLKCVDGIWAEVESNYDKGWLLYKYLERLSNAKNNSNDTIKNRKKNYIGNIRTNGLS
                     LELRNDRTLESKVITTIPDGFKVEVCYSVGKWARVNINKNEKRYSGYVYNQYIEEHLS
                     L"
     gene            1020..1418
                     /locus_tag="PC414_003789"
     CDS             1020..1418
                     /locus_tag="PC414_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2466134.1"
                     /translation="MNKKKKLIGIAISFLLIISIINPLQVEAEAIIESSVYISSIKEN
                     YTYDNTKFKTIKSKSRHKSRKYNKSKVKLKKRSIRTKKYGYQTINKYKRQNARRHNIL
                     TKLILSIIVIFLVIVLVVLIFIKRKKKKFY"
     misc_binding    1854..2117
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2268..2819
                     /locus_tag="PC414_003790"
     CDS             2268..2819
                     /locus_tag="PC414_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan transporter"
                     /protein_id="HDF2466135.1"
                     /translation="MKTNTKKLTLNAILLAMGLLIHQITPAIGLPMKPDVPLAMLFVI
                     LVLNRDDYKTCLIAGIVTGIFTALTSSFPGGQIPNVIDKTLTANIVFLLMNISYNMPF
                     IKKLAKKTQDTVVVAIIMPIGTLVSGTIFLLAAQAIVGLPGASFTALFLAVVLPAVLI
                     NLVAGIFLFKIVSLSIRRVSYQA"
BASE COUNT         1254 a          263 c          432 g          945 t
ORIGIN      
        1 tattcagata taggcaatat tagataaatc taatgacgca aaactatagg gactaaaatc
       61 tttatgtaat aaaggtcatg tcagccagtt gccaaaaaga taataattct ttttgttaaa
      121 agtattttta gagttatata ttttaataac ttaatatttg gcaagaggag ggcttaagat
      181 gatatctgaa ttaatgttaa aaaattttcg caaatctagc atcaatttta agactaaaat
      241 taaattgaat ttaagagaaa aaccagacat aaatagtttg aaattaaaaa gtataccaga
      301 aggtaaaatt gttaagttaa agtgtgtaga tggaatatgg gcagaagttg agagtaatta
      361 tgacaaaggt tggttacttt ataaatattt agaaagatta agtaatgcta aaaacaatag
      421 taatgataca ataaaaaata gaaaaaagaa ttacatagga aatataagaa caaatggatt
      481 aagtttagaa cttagaaatg atagaacatt agaatctaaa gttataacta caatccctga
      541 tggttttaaa gttgaagtat gttatagcgt tggaaaatgg gcaagagtta atatcaataa
      601 aaatgaaaaa cgatattctg gatatgtata taatcaatac atagaagagc atttaagttt
      661 ataatacgat actagcaagc tttaaatata ttaataaatc gcaaaaaaga atcccattaa
      721 tgggattctt ttttgtagaa acattataac agaactaaag agaataaatt aactaattta
      781 tttttattat aaagtaatta taaatggtaa atttaattga aagtagattt aacttatact
      841 ttatacacca agtatcataa attaatacta ctttgaaaaa tcttaaaaaa agtaaaagtt
      901 atgataataa aaataatatt aaagataata agatatataa gaaacttgaa aaaatttgta
      961 atttttagtt atatatatat tataatgaac tagttaaaaa tttttatgag ggggaaaata
     1021 tgaataagaa gaaaaaattg attgggatag ctatatcttt tctacttata atcagcatta
     1081 taaatccatt acaagtagaa gcagaagcta ttattgagtc tagtgtatac atttcttcaa
     1141 taaaagagaa ttatacatat gataatacta aatttaaaac tattaaatct aaaagtagac
     1201 ataaaagtag gaaatataat aaaagtaaag taaagttgaa aaaacgaagt ataagaacta
     1261 aaaagtatgg atatcaaact ataaataaat ataaaagaca aaacgcaaga agacataata
     1321 tcctaacaaa attaatattg tccataatag tcatattttt ggttatagtt ttagtagtgc
     1381 taatatttat taaaagaaag aagaaaaaat tttactaaaa ataattaaaa gaatatcatt
     1441 tttttaatca ttggtatcta tttaataata tgatgtaatt aaaatggagt aatattctgt
     1501 ttcaaaggag gcatataaat acaggaatga gtattaaatg atggttaatt aagtaaattt
     1561 attgataaat gtacttaatt aactttgaag aatttaattc ctcatgaatc aaaatataaa
     1621 attacatcaa cagttattca caaaattaat ataagaagaa ttagagccaa agagcagact
     1681 attagaccat taaagtaatt attcatagaa ttttgtatat gaaaagaaat gtatagtata
     1741 atttactttt tttataaaaa atagattaca aaatatattt tttataaaaa ggtattgaca
     1801 ttttttagaa aaaagaatat cattatttta acaacaaaat tcaatacaat aaattaaatc
     1861 ctatgacaag gaagagtagt taaatttagg ttcagtatag agagctgagg atggtggaat
     1921 cttagcaaga aaaaatttga tgaatggccc ttagaggaat aaattgaaat catagttttg
     1981 agagtagact ttatcgtcag tcgcacgtta tagcgaataa ggtatgtaag tacctgataa
     2041 tgagatgtat gaaaattgaa ttttatatta atttttatat aatttaggtg gcaacgcgga
     2101 taatctccgt cctattagtt taggacggag ttttttattt tactgaaaat ttaaaagata
     2161 gaaggtggtg agaaagatgt tatgaatttc aaaagctaaa gatgatgata tagtaggttg
     2221 gcaaaaaaat aaattgaaaa ttaaaaaaat aggaggaagt acaaaaaatg aaaacaaata
     2281 caaaaaaatt aactttaaat gcaatacttt tagcgatggg attattaata catcaaatca
     2341 caccagctat aggattacca atgaagccag atgtaccact agcaatgtta tttgtaatat
     2401 tggttttaaa tagagatgat tataaaactt gtttgatagc gggtatagta acaggaatat
     2461 ttacagcact tacatcaagt tttccgggag gtcaaattcc taatgtaata gataaaactc
     2521 ttacagcaaa tatagtattt ttattaatga atattagtta taatatgcct tttataaaaa
     2581 aattagctaa gaaaacacaa gatactgtag tagttgcaat aataatgcca ataggaactc
     2641 ttgtaagtgg aactatcttt ttattagcag ctcaagctat agttggtctt ccaggggctt
     2701 catttacagc tttattctta gcagtagtat taccagctgt tttaataaat ctagttgcag
     2761 gaatattttt attcaagatt gttagtctat caataagaag agttagttat caagcttaaa
     2821 cacgaaataa tattttactt tataaagcat tataataact aaataacata agaataaagt
     2881 agattataac tatt
//
LOCUS       DAMJVK010000207         2845 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.212, whole genome shotgun sequence.
ACCESSION   DAMJVK010000207 DAMJVK010000000
VERSION     DAMJVK010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.212"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            174..1097
                     /locus_tag="PC414_003791"
     CDS             174..1097
                     /locus_tag="PC414_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HDF2466136.1"
                     /translation="MGSGKVGTSLANYFLSKGYCVSGFYGRNQLSLLESTNLTKTKIY
                     SNLKDIIYENDILFITTSDDSIEIIDKKLSKFNLKHKYICHTSGSLKSSILFCSKKAG
                     ALIYSIHPIFAFSNKNTDIKKMKDICFSIEGDNEQDDLVIQNFIDDLGNQFFIRDKDT
                     SSTYHLANVLVSNLVLSLLDIGVSYFIKLGLSEEESLKAISPLVKKNIENILDNGFTK
                     ALTGPVSRGDITPIRKHLSVLDKKDEDIYKILSLNLLKIIALGNDNSLKGIKNITIEN
                     AIENLISKSEKYKEIYKLLGGKNDEKYHTDF"
     gene            1072..1899
                     /gene="panB"
                     /locus_tag="PC414_003792"
     CDS             1072..1899
                     /gene="panB"
                     /locus_tag="PC414_003792"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HDF2466137.1"
                     /translation="MKNTIQTFKNAKYEGKKLSMLTAYDYSIAKIMDECDINGILIGD
                     SLGMVIKGEENTLSVTIDEIIYHTKAVKNGVKNALIVSDMPFLSYHVSIEDAVKNAGR
                     LIKEGGAHAVKLEGGSNVIKQIESIVNAQIPVMGHLGLTPQSVNSFGGFKVQGNTSET
                     ARQLIEDAKLIEKAGAFSIVLEGVPTKIAEMVTNSISIPTIGIGAGINCDGQILVYQD
                     MLGMFGDFVPKFVKQYANIGDIIKDSIKNYILEVNTGAFPQEKHSFSINESELEKLY"
     gene            1918..2766
                     /locus_tag="PC414_003793"
     CDS             1918..2766
                     /locus_tag="PC414_003793"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HDF2466138.1"
                     /translation="MLVKEIKLLRNIIKDWRKHGYSIGLVTTMGFLHEGHQSLIKKAV
                     KENDKVVVSIFVNPTQFGPNEDFNSYPRDIDKDFKYCMDSGATVVFNPSPEEMYLKGN
                     CTTINVSGLTDFLCGAKRPVHFGGVCLVVSKFLNIVTPDKAYFGEKDAQQLAVIKRMV
                     KDLNIDTEIIGCPIIRENDGLAKSSRNTYLSEEERKSALILNKSLSLAKEELVKGNLN
                     PENIKELITAKINSEHLAKIDYVEIVDSETLQPVKQIEHSILVAIAVFIGKTRLIDNF
                     TFELNI"
BASE COUNT         1141 a          341 c          461 g          902 t
ORIGIN      
        1 ttgtcaattt tttatgaaaa tggtaagata tttatggatg gtatcttgtt ataagaacta
       61 tactttaaat gtttttaatt attttgtctt atgtaactca tatcctcagg aatgagatta
      121 gaaattaaga tgttgtatca caaagtggag gtgatactat taaaataggc tttattggtt
      181 ctggaaaagt tggaacatct ttggcaaatt attttttatc aaaaggttat tgtgtttcag
      241 gcttttatgg aaggaatcaa ttatctttat tagaatcaac aaatttaaca aaaacaaaga
      301 tttacagcaa tttgaaagat ataatttatg aaaacgatat attatttatt acaacatcag
      361 atgattctat tgaaattata gacaaaaaac tttccaaatt taatttaaaa cataaataca
      421 tttgccacac aagtggctct ttaaaatcta gtatattgtt ctgttcaaaa aaagcaggtg
      481 cattaattta ttctatccat cctatatttg cattttccaa taaaaatact gacataaaaa
      541 aaatgaaaga catttgtttt tccatagaag gtgataatga acaagatgat ttagttattc
      601 aaaatttcat agatgatttg ggaaaccaat tttttataag agataaggat acttcatcca
      661 cttatcattt agctaatgtt ttagtttcaa atttagtatt atctttacta gatataggag
      721 taagttactt tattaaatta ggtttatctg aagaggaatc tttaaaagct atcagtcctt
      781 tagtaaaaaa aaacatagaa aatatacttg ataatggctt tactaaagct cttactggtc
      841 ctgtttctcg tggagatata acaccaataa ggaaacatct atctgtattg gataaaaaag
      901 atgaggatat ttacaaaata ctatccttaa acctactaaa aataattgcc ttaggaaatg
      961 acaattcatt aaaaggtata aaaaatatta ctatagaaaa tgcaatcgaa aatttaataa
     1021 gtaaatctga aaaatacaaa gaaatttaca aacttttagg aggaaaaaac gatgaaaaat
     1081 accatacaga cttttaagaa tgctaaatat gaaggaaaaa aactatctat gcttactgct
     1141 tatgactact caattgcaaa aataatggat gagtgtgata taaatggtat tttaatagga
     1201 gattcattag gaatggtcat aaaaggtgaa gaaaacactc tatctgttac catagacgaa
     1261 attatatatc atacaaaagc tgtaaaaaat ggcgtaaaaa atgccttaat cgtaagtgat
     1321 atgccttttc tatcttacca tgtttctata gaagatgcag ttaaaaacgc tggtagactt
     1381 ataaaagaag gaggagctca tgctgtaaag ttagaaggtg gctcaaacgt tataaagcaa
     1441 atagaaagca ttgtaaatgc tcaaatacct gtaatggggc atttaggatt aactcctcaa
     1501 tcagtaaatt catttggtgg gtttaaggtt caaggaaata ccagtgaaac agcaagacaa
     1561 cttattgaag atgctaaatt aatagaaaaa gcaggtgcat tctctattgt ccttgaagga
     1621 gtgcctacga aaatagctga gatggtaact aattctattt caattcctac aataggtata
     1681 ggggcaggaa ttaactgtga tggacaaatt ttagtttatc aagatatgtt gggaatgttt
     1741 ggagattttg ttcctaagtt tgtaaagcag tatgcaaata taggagatat aataaaagat
     1801 tcaattaaaa attacatcct agaagttaat acaggtgcat ttccacaaga aaaacacagc
     1861 ttttcaataa atgaatctga gttagaaaaa ttatactaat attaaggatg gtacaaaatg
     1921 cttgtaaaag aaattaaatt actaagaaat ataatcaaag attggagaaa acatggctat
     1981 tctataggcc ttgttacaac aatgggattt ttacatgaag gacaccaaag tcttataaaa
     2041 aaagctgtaa aagaaaatga taaagtagta gtgagtatat ttgtaaatcc aactcaattt
     2101 ggaccaaatg aagattttaa tagctatcca agagatatag ataaagattt taaatattgt
     2161 atggatagtg gagcaacagt agtctttaac ccatcaccag aagaaatgta tttaaaaggt
     2221 aattgtacta ccattaacgt ttctggactt acagattttc tttgtggagc taaaagacct
     2281 gttcattttg gaggtgtctg cctagttgtt tcaaagtttc taaatatagt aacacctgat
     2341 aaagcttatt ttggagagaa ggatgcccaa caattagctg taattaaaag aatggtcaag
     2401 gatttaaaca tagatacaga aataataggc tgtcctataa ttcgtgaaaa tgatggatta
     2461 gcaaaaagtt cgagaaatac atatctctct gaggaagaac gaaaatctgc tctaatatta
     2521 aataagagct taagtttagc aaaagaagaa ttagtaaaag gcaatctaaa tccagagaat
     2581 ataaaagaat taataacagc taaaataaat tctgaacact tagcaaaaat agactatgtt
     2641 gaaattgtag atagtgagac attacaacct gttaagcaaa tagaacattc tatacttgtg
     2701 gcaattgctg tgtttatagg aaaaaccaga ctaattgata attttacatt tgagttaaat
     2761 atatagtcta taattttata taatatatct aaaaaataat agaataccta aaaagtaata
     2821 aattacctaa aaaataataa attgc
//
LOCUS       DAMJVK010000208         2829 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.213, whole genome shotgun sequence.
ACCESSION   DAMJVK010000208 DAMJVK010000000
VERSION     DAMJVK010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.213"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(388..1095)
                     /locus_tag="PC414_003794"
     CDS             complement(388..1095)
                     /locus_tag="PC414_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDF2466139.1"
                     /translation="MIFTKSNKYDKDFLMKNMMGPNCIKILEELTSKIKLEKGMRILD
                     LGCGKGISSIFLAKEFDATVFATDLWIEPTENYKRFKEFKLDDKIFPIQAEAHELPYA
                     EGFFDAVISIDSYHYFGNKEGFLDNHISPLVKEGGILAMAMPGLKEDFVDCIPDELIP
                     FWQDNMNFHSITWWNKLWSESESVIVEKCEALNCHDEAWKDWINCDNSYAINDKKMME
                     VENGKYFNTISLIARTK"
     gene            complement(1898..2767)
                     /locus_tag="PC414_003795"
     CDS             complement(1898..2767)
                     /locus_tag="PC414_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HDF2466140.1"
                     /translation="MVIIKAIELYKKLDLEFNIKNINDDWSFMNFENDFITPEFRKKY
                     IGLVLDNAQNINKVYTTTFPDKEIIKQVIDKDEKDILIFSHHAMGYIASDEGFPFHDI
                     PLSYMEEMKNRRISFYVLHSPLDNYSDYSTSVSFAKAMGLEIVKPFCKYDDKIEVGVI
                     CKTSLKSIEEIKELIKKSVGHDVKLYDYGDSVLKDGLVAIAAGGGSYPFVAREVAELG
                     INLYITGFTKPLKHFEPVLEFHQIAKDNLINVIGATHYSTEKFACMGMVDYFKNLGLE
                     SEFLQGKYYLEDL"
BASE COUNT          994 a          458 c          244 g         1133 t
ORIGIN      
        1 aaatttgatt taaagtataa taaaatatat aaaaaaatat ataaaatggt actataaaat
       61 ttttaatctt catcaatacc ttttttgctt tcattgttaa aactattttt tctattagtt
      121 tatttcttat aaaacaaaca tttagtatta aaatatatat cacacaatag tatttgcaat
      181 ttaactttaa aaaaataaaa atacccaact gacctaagca actcgattgg gtataaataa
      241 tctaaaaaat catttttgta taaactcttt atttaactta catttttatt taccttctac
      301 tttaattcaa cttatatttc tatgcttctt atactctcat tcaacttata tttctactta
      361 tcttatactt ttataaattc atcacaatta tttagttctc gctatcaaag aaattgtatt
      421 aaaatacttt ccattctcta cttccatcat ttttttgtca tttatagcat atgaattatc
      481 acaatttatc caatccttcc aagcttcatc atgacaattt aaagcttcac atttctctac
      541 aatcacagac tccgattcag accaaagttt attccaccat gtaattgaat ggaagttcat
      601 attatcttgc caaaatggaa tgagttcatc tggtatacaa tccacgaaat cttcttttaa
      661 tccaggcatt gccatagcaa gtattccccc ctcttttaca agaggtgata tatgattgtc
      721 taaaaatcct tctttgtttc caaaatagtg ataagaatcg atacttatca ccgcatcaaa
      781 gaaaccttct gcataaggta gttcatgtgc ctctgcctgt attggaaata ttttatcatc
      841 caatttaaac tctttaaatc ttttatagtt ttcagttggt tctatccata aatctgttgc
      901 aaatacagta gcatcaaatt cctttgctaa aaatatcgaa gatataccct taccacaacc
      961 taagtctaaa atacgcattc ccttttctag ttttatctta cttgttaatt cttccaatat
     1021 ttttatacaa ttaggtccca tcatattttt catgagaaag tccttatcgt atttattact
     1081 ttttgtaaat atcattctat ctttcctttc cctatcagtt attatttatt aaattttata
     1141 cgtttgattt tttatttttt atctgcttct tatattgagc aatctctttt ttattcattc
     1201 tttaacagac aattttaatg tgtaatttta tccactctgt gatagtaact ttaatgaata
     1261 atcttatcta ctgtgatagt tattttagtg agtaatttta tctactccac agcaatcgct
     1321 ttaatgagta atcttatcca ctctatgata gttattttag tgagtaattt tatctactcc
     1381 acagtaatcg ctttaacgag taatcttatc tactctataa tagtcacttt aatgagtaat
     1441 cataattttt atatcatttt agatacttac tctaataaga attcattttc taatacttaa
     1501 taagttttaa ttacttccac tttaacctat atactaatta acttatttaa tttagtgcat
     1561 atttgacact ttacctttta ttataccatt tattatcata aatataataa ttctattttt
     1621 ttaaaataaa tatttataat aatctaaaat aaatatttat aaattctaat caaccgtaaa
     1681 taataatcta aaatacatca aaaaataaac taaaaaatat cgaaaaataa tttaaaagac
     1741 atcaaaacta aatcttctaa tcagcaatta aaagtctatt ttcttagttt aaaaaataaa
     1801 gatagatttt ttcaaaacac tggataaata ggtaaaaaaa taaacagtta gtaaaataaa
     1861 tttttactaa ctgtttattt ttattgttta atatgtccta taaatcctct aagtaatatt
     1921 ttccttgcaa aaattctgat tctaaaccta gatttttaaa ataatcaacc atgcccatac
     1981 acgcaaattt ttctgtagaa taatgagtag cacctataac attaattaaa ttatcttttg
     2041 ctatttgatg gaactctaat actggttcaa aatgttttaa aggcttagta aacccagtaa
     2101 tatatagatt tataccaagt tcagcaactt ctcttgctac aaatggataa cttcctcccc
     2161 ctgctgcaat agcaactaaa ccatctttta atacagaatc tccataatca tacaatttca
     2221 catcatgacc aacagatttt ttaattaatt ccttaatctc ttctattgat tttagtgatg
     2281 ttttacatat aactcctacc tcaattttat cgtcatattt acaaaatggt ttgactattt
     2341 ctaaacccat agcttttgca aaactaacag aagttgaata gtcactatag ttgtctagtg
     2401 gagaatgtaa tacataaaaa gaaattctac gatttttcat ctcttccata taagatagtg
     2461 gtatgtcgtg aaatggaaaa ccttcatctg atgctatgta tcccattgca tgatgtgaaa
     2521 aaattaaaat atctttttca tctttatcta taacttgctt aattatttct ttatcaggaa
     2581 aagtagttgt gtatacttta ttaatatttt gagcattatc taatactaat cctatgtatt
     2641 tttttctaaa ttctggagta ataaaatcat tttcaaaatt cataaaactc caatcatcat
     2701 taatattttt aatattaaat tctaaatcta attttttata aagttcaatt gccttaataa
     2761 taaccactcc tttataataa tattgaatta ctacatatta atatagaaat ttccgataat
     2821 aaactttat
//
LOCUS       DAMJVK010000209         2566 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.214, whole genome shotgun sequence.
ACCESSION   DAMJVK010000209 DAMJVK010000000
VERSION     DAMJVK010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.214"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(296..1051)
                     /locus_tag="PC414_003796"
     CDS             complement(296..1051)
                     /locus_tag="PC414_003796"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDF2466141.1"
                     /translation="MLKVIISGYNGTMGRVLAECVKKDNELELVCGVARSADEKGHHS
                     DTKLYKTMTDVTEKTDVIIDFSHHETLEPILSYALKNNTPVVLATTGYDENDLNKIEE
                     ASKNIPILLSSNTSLGVNILIKLVKDASKMLEGFDIEIIEKHHNRKEDAPSGTATMIA
                     NAIREVLPESENTHGRYGRDCKRKPNEIGIHSIRGGNIISDHDVVFAGDNEVLTLSHH
                     SQSDEVFANGSIVAAKFLIGKQSGLYKMSDILG"
     gene            1491..2480
                     /locus_tag="PC414_003797"
     CDS             1491..2480
                     /locus_tag="PC414_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HDF2466142.1"
                     /translation="MLKEEKTYQEKDAKVFNGVAALIMILLFLVVSIGIMVFGIVKLD
                     SGFTGIGSLMLILGLVFIVVDFILFFGLKMINPKEAIVLVLFGNYYGTIKKEGYYWVN
                     PFCSAINPAVSRINVSKSSSDEKVDISTNSRGKKVSLKTMTLNNEKQKVNDLLGNPII
                     IGVVVIWKVIDATKAVFNVDNYNTFLSIQCDSTIRNVSRLYPYDVSEDGDEKSLRGSS
                     QEIADRLKDELQSRVDIAGIEVCEVRITHLSYAPEIAAAMLQRQQAEAIIAARKKIVE
                     GAVSMVEMALTNLSENNVVTLDEERKAAMVSNLLVVLCGNKDAQPVVNSSSIY"
BASE COUNT          951 a          314 c          379 g          922 t
ORIGIN      
        1 aggataatat ttaataggtt tatatgatga aaagattata aaataataaa caatatgatt
       61 ccatatacca ataaatagga taatatttaa taggtttata tgatgaagag attataaaat
      121 aataaacaat ataattccat ataccaataa ataagataat ctttaagaaa aatgattata
      181 tttaaaaggt aaacataaaa tttataaact tttagaatag aaatctcaat caattagata
      241 ttcaaattga tgaatatgaa tacctaaata gattgagatt tattatgtac aaaacttatc
      301 ctaatatatc tgacatttta taaagcccag attgcttacc aattaaaaat ttagctgcaa
      361 ctatagaacc gtttgcaaat acttcatcag attgagaatg atggcttaat gtaagaactt
      421 cattatcacc tgcaaataca acatcatggt cgctgattat attgccacct cttatagagt
      481 gtattccaat ttcatttggt tttcttttac aatctctacc atatctacca tgtgtatttt
      541 cactttcagg caagacttct ctaattgcat tagctatcat agtagcagtt ccactagggg
      601 cgtcttcttt tctattgtga tgtttttcta tgatttcaat atcaaaacct tctaacatct
      661 ttgaagcatc cttaactaat tttattaata tattcactcc aagagatgta tttgatgata
      721 ataatatagg aatgtttttt gatgcttctt ctattttatt taagtcattt tcatcatatc
      781 cagtagtagc taatactaca ggtgtattat tttttagagc atatgacaat attggctcta
      841 aagtctcatg atgtgaaaag tctattatta catcagtttt ttcagttaca tcagtcattg
      901 ttttgtaaag ctttgtatca ctatgatgac ctttttcatc agcagaccta gctacaccac
      961 aaaccaactc taattcatta tcttttttta cacattctgc taaaactctt cccattgttc
     1021 cattatagcc actaattata actttcaaca taaattcctc ctatgaaatt aataaaaatt
     1081 tgttatattt ctataagttt attttatcac taatataatt tatataaaat aatattagtg
     1141 ataataagct tacaatttat cttacattaa aaaatttaag tggatttttc aaaatataga
     1201 taaatttctt tttcattgta taaatgataa aaatatattt gacttcatac caaaaagagt
     1261 gccccatgaa ctaaaaagtt aattttgaaa taccatattc atatatcata tgatttattt
     1321 ggaaatcaat attaatacat taaaataact aaatacatgt atgcataaag atgtataaaa
     1381 aatatataaa catatagtat taattttaaa ttcaacaatt taattgttaa aagtgttatt
     1441 attaaattag aaatatctta acttaatata ctgagatggg agtgatagat atgttaaaag
     1501 aagaaaaaac ttatcaagag aaagacgcaa aagtattcaa tggagtggca gctttgataa
     1561 tgattttatt atttttagta gtttcaatag gtatcatggt ttttgggata gtaaaacttg
     1621 atagtggatt tactggaata ggttcactta tgttaatttt gggacttgta tttatagtag
     1681 tagattttat cctatttttt gggctaaaaa tgattaatcc taaagaagct atagttctag
     1741 ttttgtttgg taactattat ggaactataa aaaaagaagg gtactattgg gtgaatccat
     1801 tttgttcagc aataaaccca gcagtatcaa gaattaatgt atcgaaatct tcatctgatg
     1861 aaaaggtaga tatatcaact aattcaaggg gcaaaaaagt atctttaaaa acaatgactc
     1921 ttaataatga aaagcagaaa gtcaatgatt tattgggaaa tccaattata ataggagtgg
     1981 ttgtaatttg gaaggttata gatgcaacaa aggcagtatt taatgtagat aattataata
     2041 cattcttatc tattcagtgt gattctacta ttcgcaatgt ttcaagactt tatccatatg
     2101 atgtatctga agatggagat gaaaaatctc ttcgtggtag tagtcaggaa atagcagata
     2161 gattaaaaga tgaattacaa tcaagggttg atattgcagg aattgaagtg tgtgaagtga
     2221 gaattactca tctttcttat gcaccagaga ttgcagcagc aatgttacaa cgtcaacaag
     2281 cagaggctat aatagcagca cgtaaaaaga ttgttgaggg ggctgtaagt atggtagaga
     2341 tggcattgac taatttaagt gaaaataatg ttgtgacttt ggacgaagaa cgtaaagcag
     2401 ctatggtaag taatctgtta gttgtgcttt gtggcaataa agatgcacaa ccagtggtaa
     2461 acagcagttc tatttattaa attataaaaa gtttaatagt ttattttatt gatatataga
     2521 aaatttagta gttcatttta ttaaattata gaaagattaa tggttt
//
LOCUS       DAMJVK010000210         2482 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.215, whole genome shotgun sequence.
ACCESSION   DAMJVK010000210 DAMJVK010000000
VERSION     DAMJVK010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.215"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(125..2170)
                     /gene="bclA1"
                     /locus_tag="PC414_003798"
     CDS             complement(125..2170)
                     /gene="bclA1"
                     /locus_tag="PC414_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA1"
                     /protein_id="HDF2466143.1"
                     /translation="MRNIILYLNDDTFISKKYPDKNFSNLDYCLIGSKCSNSFVKEKL
                     ITFFKVRIPDILKDKSILKAELFIHIDSNKNHIFKEKVDIEIKRISEYYNLRTITWND
                     RVSMENIRGYLPVGISDTSNYICLNITGTIKAWAMNKYPNYGLALSLNYPYQILEFTS
                     SRGCNKPYILVTFEDRIIDNCYPKCECLPIRITGPMGPRGATGSTGPTGVTGPTGSTG
                     ATGSIGPTGPTGNTGATGSIGPTGVTGPTGSTGATGSIGPTGVTGPTGNTGVTGSIGP
                     TGATGPTGNTGVTGSIGPTGVTGPTGNTGEIGPTGATGPTGVTGSIGPTGATGPTGEI
                     GPTGATGATGVTGSIGPTGATGPTGATGVTGEIGPTGEIGPTGATGPTGVTGSIGPTG
                     ATGEIGPTGEIGPTGATGPTGVTGSIGPTGATGPTGATGEIGPTGATGPTGNTGVTGE
                     IGPTGATGPTGNTGVTGEIGPTGATGPTGVTGEIGPTGNTGATGSIGPTGVTGPTGAT
                     GSIGPTGATGATGVTGPTGPTGATGNSSQPVANFLVNAPSPQTLNNGDAITGWQTIIG
                     NSSSITVDANGTFTVQENGVYYISVSVALQPGSSSINQYSFAILFPILGGKDLAGLTT
                     EPGGGGVLSGYFAGFLFGGTTFTINNFSSTTVGIRNGQSAGTAATLTIFRIADTVMT"
BASE COUNT          565 a          558 c          381 g          978 t
ORIGIN      
        1 tatgaatata ttttattatt attgttattt tattcattct aaaataggta aaactcaata
       61 tataaaaata cttttgtcat ttaaataaag cctaaatagt ttttaagatt attttagaca
      121 cgttttaagt cataacagta tcagctattc taaatatcgt caaagtagcc gcagttcctg
      181 ctgattgccc atttcgtatc cctactgttg tagatgaaaa attatttatt gtaaaagtag
      241 tcccaccaaa taaaaaacca gcaaaatatc cagaaagtac tcctccgcct cctggctcag
      301 tagtaagccc tgccaaatct tttcctccta aaattgggaa taggatagcg aaagaatatt
      361 gatttatact tgatgaacct ggttgtaatg ctactgaaac tgatatataa tacacaccat
      421 tttcttgtac tgtaaacgta ccatttgcat ctactgttat acttgaacta tttcctatta
      481 ttgtttgcca acctgttata gcatctccat tatttagtgt ttgtggagat ggtgcattta
      541 cgaggaagtt agcaactggc tgagaggaat tgcctgttgc tcctgttgga cctgttgggc
      601 ctgttactcc tgtcgctcct gttgctcccg ttggtcctat acttcctgtc gctcctgttg
      661 gacctgttac tcccgttggc cctatacttc ctgtcgctcc tgtatttcct gttggcccta
      721 tttctcctgt cactcctgtt gggcctgttg ctcccgttgg tcctatttct cctgtcactc
      781 ctgtatttcc tgtcggaccc gttgctcccg ttggtcctat ttctcctgtt actcctgtat
      841 ttcctgttgg acctgttgct cccgttggtc ctatttctcc tgtcgctcct gttgggcctg
      901 ttgctcccgt tggtcctata cttcctgtca ctcctgttgg gcctgttgct cctgttggtc
      961 ctatttctcc tgttggtcct atttctcctg tcgctcccgt tggtcctata cttcctgtca
     1021 ctcctgttgg gcctgttgct cccgttggtc ctatttctcc tgttggccct atttctcctg
     1081 tcactcctgt cgctcctgtt ggacctgttg ctcctgttgg tcctatactt cctgtcactc
     1141 ctgtcgctcc tgttgctccc gttggtccta tttctcctgt tggtcctgtt gctcctgttg
     1201 gtcctatact tcctgtcact cctgttggac ctgttgctcc cgttggtcct atttctcctg
     1261 tatttcctgt tgggcctgtt actcctgttg gtcctatact tcctgtcact cccgtatttc
     1321 ctgtcgggcc tgttgctccc gttggtccta tacttcctgt cactcccgta tttcctgtcg
     1381 gacctgttac tcctgttggt cctatacttc ctgtcgctcc tgtacttccg gttgggcctg
     1441 ttactcccgt tggccctata cttcctgttg ctcctgtatt tccggttggg cctgttggtc
     1501 ctatgcttcc tgtcgctcct gtacttccgg ttgggcctgt tactcccgtt ggtcctgtac
     1561 ttcctgtcgc tcctcttggt cccattggac ctgtaattct aattggaaga cactcacatt
     1621 taggataaca attatctata attctatctt caaatgttac aagtatatac ggtttgttac
     1681 aacctctact agatgtaaat tcaagaatct gataagggta atttaaagat aaagctaacc
     1741 cataattagg atatttattc attgcccatg cttttatagt tcccgtaata tttaaacaaa
     1801 tatagttgga tgtatcactt atcccaactg gtaaatatcc cctaatattt tccatagaca
     1861 ctctatcatt ccatgttata gttcgtaaat tataatattc acttattctt ttaatttcaa
     1921 tatctacttt ttctttaaaa atatgattct tatttgaatc aatatgaata aataactctg
     1981 cttttaatat acttttgtct tttaatatat ctggtattct cactttaaaa aaagtaatca
     2041 acttttcttt tacaaaacta tttgaacatt tacttcctat taagcaataa tctaaattac
     2101 taaagttttt atctggatat tttttagata taaaagtatc atcatttaaa taaagtataa
     2161 tatttctcat ttataatccc tccattttgg gtctatatca tactatgttt aaaatacatg
     2221 cttagttaca atataattaa aatttatagt attaaatttt tatataataa tgctgatttc
     2281 tacaaaaaaa gaacatgtca ttagaaattt ctaatagcat gttctttttc attattatat
     2341 attacacttt aaattattcc ttgactggct tagctggtgt aaactaaacc agtctttcta
     2401 aaatattaag cacttttttc ttattatgtt ttttaggatt tattgtctat attattgtaa
     2461 accaaagtta taaaattgta aa
//
LOCUS       DAMJVK010000211         2473 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.216, whole genome shotgun sequence.
ACCESSION   DAMJVK010000211 DAMJVK010000000
VERSION     DAMJVK010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.216"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            165..611
                     /locus_tag="PC414_003799"
     CDS             165..611
                     /locus_tag="PC414_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF2466144.1"
                     /translation="MDFKNLQYESLSYIICNLQKNFKLYCEKCLKPYKLTNGLYFYLI
                     YINKNRNCSLNDVSTEFEVDKAHTTRTISRLEQDGYIEKIQNPNDSRAFQLRVTDKGE
                     EVLGDIKNIFSKWDNHIKKEFSDTEYKELVKNLHVVKDIKTAVEEE"
     gene            613..1707
                     /locus_tag="PC414_003800"
     CDS             613..1707
                     /locus_tag="PC414_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HDF2466145.1"
                     /translation="MYCNLLKPIKIGNLELKNRVSFAPTSMGLKLEEKIKKFSDIAKS
                     GVALITLGDVSIRPSFHKVAISLSDEDGVMKYKKIVDEIHNSGAKVSAQLFCSDYDVN
                     LIKDTMNMGITSHDEIKKIMNDGVKDYITNMPKEEIKNIINLFKVTALNAKKAGFDMI
                     QIHGDRLVGSFSSSIFNNRNDEYGRTCDNRSRFASEIISSIRDEVKDIPIDYKFAIRQ
                     ENPHYGNAGVLLSEVEYFVKKFESLGVNSFHVTLANHSKLEDTIPTNNHPYFKEEGCF
                     LYLADEVKKHTNLPVCGVGKLSSPDFIESIISNNRVDMVSMSRQLLADSNWLQKVKDG
                     RVDEIKKCCYCNKKCADALQTRSQFGCILD"
     gene            1795..2325
                     /locus_tag="PC414_003801"
     CDS             1795..2325
                     /locus_tag="PC414_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2466146.1"
                     /translation="MKSLVLYSSLTGNTKKIAYTIYDEIQEEKDIKDVNELVDYGIDY
                     ENYDIVFLGYWVDKGICDKNSKQVLENIHNKKIALFGTMGASKKGSYGASIIEKIESI
                     IPKDNEILGSFICQGKIAEGLKAKYKEMLKLSPDNEHIRQQLNNHEESQSHPDEQEIY
                     EASMFAKNMMIKASIV"
BASE COUNT         1030 a          240 c          424 g          779 t
ORIGIN      
        1 aaattccaat ttctaatttt atcatgatta ttttatataa tagtttttta aagaattaat
       61 aaaacataga tttagagaat aaaatattct tgactatatt aattgtttaa tataatatag
      121 ttgatatagt caaacatatt ataattatag aagggaaatt taagatggat tttaaaaatt
      181 tacagtatga aagtttgtca tatattattt gtaacttgca aaaaaacttt aaactttatt
      241 gtgaaaaatg tttaaaacca tataaactta cgaatggtct atatttctat ttaatttata
      301 ttaataaaaa taggaattgt tctttgaatg atgtttctac agaatttgaa gttgataaag
      361 ctcatacaac aagaactata tctaggttag aacaagatgg atacatagaa aaaatccaaa
      421 accctaatga tagtagagct tttcagttga gagtaacaga taagggtgag gaagtattag
      481 gggatataaa aaacatattt tcaaagtggg ataatcacat aaaaaaagag ttctcggata
      541 cagaatataa agaactggta aagaatttac atgtggtaaa ggatataaaa acagctgtgg
      601 aggaagaata ggatgtattg taatttatta aaaccaatta aaattggaaa tttggaatta
      661 aaaaatagag tatcttttgc acctacaagc atgggtctta aattggaaga aaaaattaaa
      721 aagttttctg atattgctaa gtcaggagta gctctgatta ctctaggaga tgtatctatt
      781 agacctagtt ttcataaggt agctataagc ctatcggatg aagatggagt catgaaatac
      841 aagaaaattg tagatgaaat acacaattct ggtgcaaagg tatcagcaca actattttgt
      901 tcagattatg atgttaattt aattaaagat actatgaata tgggaattac ttcacatgat
      961 gagataaaaa aaataatgaa tgacggagta aaagattaca taacaaatat gcccaaagaa
     1021 gaaattaaaa atattataaa tttatttaaa gtaacagcat tgaatgcaaa aaaagcaggt
     1081 tttgacatga ttcaaattca tggtgatagg cttgtaggaa gtttttcatc aagtatattt
     1141 aataatagaa atgatgaata tggtagaacg tgtgataata gaagtagatt tgcaagcgaa
     1201 ataattagta gtatccgaga tgaagttaag gacattccaa tagactataa atttgcaata
     1261 agacaagaaa atcctcatta tggaaatgca ggagttttac tttcggaagt tgagtatttt
     1321 gttaaaaaat ttgaatccct aggtgtaaac agttttcatg taacacttgc aaatcattca
     1381 aaattagaag atactatacc aaccaataat catccttact ttaaagaaga aggctgtttt
     1441 ttatatttag ctgatgaagt aaagaaacac acaaatcttc cagtatgtgg ggttggaaaa
     1501 ttgtctagcc cagattttat agaaagtatt atttcaaata atagagtaga tatggtttct
     1561 atgtcaagac aattactagc agatagtaat tggttacaga aagtaaaaga tggaagagta
     1621 gatgagatta aaaagtgctg ttattgtaat aagaagtgtg cagatgcttt acaaacacgc
     1681 tctcagtttg gatgtatact agattagaat atccttataa aaataaataa tttgttaagg
     1741 ttaatatatt tttggtgatt tagataaata tttagataaa gaggaggaga catcatgaaa
     1801 agtttagttt tatattcaag tttaactgga aatactaaaa agattgctta tactatatat
     1861 gatgaaattc aagaagaaaa agacattaaa gatgtaaatg aattagttga ttatggtatt
     1921 gactatgaaa attatgatat agtattctta ggctattggg tggacaaggg aatttgtgat
     1981 aaaaattcaa aacaagtgct tgaaaatatt cacaataaaa agatagcact atttggaaca
     2041 atgggagcaa gtaaaaaagg aagttatgga gctagtataa tagaaaagat agaatcaatt
     2101 atcccaaaag ataatgaaat actaggttca ttcatatgtc aaggtaagat agcagaagga
     2161 ttgaaagcaa aatataaaga gatgttaaaa ctttctcctg acaacgaaca tattcgccaa
     2221 caattaaata accatgagga atctcaaagt catccagatg aacaagaaat atatgaagcc
     2281 agtatgtttg caaaaaatat gatgattaaa gctagtattg tgtagtagtt aaacaataag
     2341 ttcatataag atatagaata aataatttag taaaaaagta tagcaaataa tttgctatgc
     2401 tttttttatg aaatcaaaaa aataataaaa atacttgact gtaaaccttg tttatacatt
     2461 attttataga aaa
//
LOCUS       DAMJVK010000212         2419 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.217, whole genome shotgun sequence.
ACCESSION   DAMJVK010000212 DAMJVK010000000
VERSION     DAMJVK010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.217"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            54..527
                     /locus_tag="PC414_003802"
     CDS             54..527
                     /locus_tag="PC414_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2466147.1"
                     /translation="MFKEYLIRELKEDDIKNALDLIWNVFEEFESPDYCDEGITTFHQ
                     FIEYNSIIEKIKEKTLFFWGCFDSKKLIGVLAIRNLNHICMLFVDKNYHKHGVAKKLM
                     TTGIQYCICNKFNLTEITVNSSPYAVEVYHKLGFKDTAVEQIVDGIRFTPMKLLI"
     gene            complement(896..1453)
                     /locus_tag="PC414_003803"
     CDS             complement(896..1453)
                     /locus_tag="PC414_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2466148.1"
                     /translation="MRVSREIVIQATSDIVNKDGLNKVSLKVIAEKLGIRTPSLYNHI
                     ESLDDLLLEVAHKGMREMNSQMIKATIGNFGDTAIKCISIAYFNFIISNPGVYETIQW
                     ATWHGNNETLEIFDEYKSLLEKLILSCNLNTKNTGEVLNLIMSVLHGYSTLQLGKSLL
                     SKEEAIKGLADSIDTVLLGIHKKYD"
     gene            1711..2409
                     /locus_tag="PC414_003804"
     CDS             1711..2409
                     /locus_tag="PC414_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2466149.1"
                     /translation="MEKKYFEIHGVPSILWGNPSSKLYIYIHGQCGNKEEAEAFANIA
                     VPHECQVLSVDLPEHGERKREKNSFNPWHVVPELVSIMEYAKCHWNQISLYANSIGAW
                     FSMLSFEKENLRECIFVSPILDMQQLISNMMLWANVSEEQLKHELIIPTSFGHTLSWE
                     YLQYVKQHPITQWNVPTKILYGKHDELTEIGVVEKFVEKFNCNLTIIENGKHWFNTPQ
                     ELNSLYEWLNTLVK"
BASE COUNT          893 a          319 c          300 g          907 t
ORIGIN      
        1 tttatttaag atatataatt ataattaaaa taataataaa ggtgttgttt ataatgttca
       61 aagaatactt aataagagaa ttaaaagaag atgatattaa aaatgcatta gacttaatct
      121 ggaacgtatt tgaagaattt gaatctccag attattgtga tgaaggaatt acgacatttc
      181 atcaatttat agaatataac tctattattg aaaaaatcaa agaaaaaact ctcttctttt
      241 ggggttgttt tgactctaaa aaacttattg gagtcttagc tattagaaat ttaaatcata
      301 tatgtatgtt atttgtagat aaaaactatc ataaacatgg tgttgcaaaa aaattaatga
      361 ctacaggcat acaatactgt atttgtaata aatttaattt aactgaaata actgtaaatt
      421 catcacctta tgctgttgaa gtttatcata aattaggatt taaagatact gctgttgagc
      481 aaattgttga cggtatacgt tttacaccta tgaaattgtt aatataataa attaaataaa
      541 aagcaaagtc tcttaaatat tttatagtta ctttgctttt aaaccaagat ttaaaataat
      601 actatctttt ttgtttttta ataatatact ttaaaatcct tactaatatg aactataatt
      661 tattttttga tatccttttt gattaacata taaattaatt caattattaa acataaacta
      721 tgttgtttct ctttatatct cttctttgta aacaatattg ggcatttgaa aataaatatt
      781 cttaaaatat actgttctcc tgttgatttt cctttggtac aaatgataaa aatttaattt
      841 acatgcccca taatgaagtg ttcaataaat aagttaaaat aatttattta cctccttaat
      901 catatttttt atgaatacct aataacacag tatcaataga atcggctaaa ccttttatag
      961 cttcttcttt actcaaaagt gatttcccaa gttgcaaagt cgaataacca tgaagtacac
     1021 tcattattaa gtttaatact tcacctgtat tctttgtatt tagattacaa gaaagaataa
     1081 gtttttctaa taaggattta tattcatcaa agatttctaa tgtttcatta tttccatgcc
     1141 aagtagccca ctgaattgtt tcataaacac caggattaga aattataaaa ttaaagtatg
     1201 ctatacttat acactttata gctgtatctc caaaatttcc aatagtcgcc tttatcattt
     1261 gactattcat ctctctcatt cctttatgag ctacttcaag taataaatca tctagacttt
     1321 ctatatgatt gtacaaagat ggtgtacgta ttcctagctt ttctgcaata acttttaaag
     1381 aaactttgtt aagcccatct ttattaacta tatctgatgt agcttgtata actatttctc
     1441 tactaacacg catgattttc cctctttcat ttaaaattaa ttactttatt agtttagcta
     1501 atctaattag tttcgtcaat tattgtaatt tataaaattc tttatactat tataaaatct
     1561 tgacagttta attataattt aatataatac taattgaatt agttttaata ctaatataat
     1621 tagtattata ttaaattcta tattacaaca ttattttata gtttgattaa tttaacaatc
     1681 actaatatat caaactgaaa ggagattttt atggaaaaaa aatatttcga aattcatgga
     1741 gttccttcaa tcctttgggg aaatccttca agtaaattat atatttacat acatggacaa
     1801 tgtggcaata aagaggaggc tgaagctttt gctaatattg cagttccaca tgagtgtcaa
     1861 gttttaagtg ttgatttgcc tgaacatgga gaacgtaaaa gagaaaaaaa ttcatttaac
     1921 ccatggcatg ttgttccaga attagtatct attatggaat atgcaaagtg ccactggaat
     1981 caaatttcgt tatatgcaaa tagcattgga gcatggttta gtatgttaag ctttgaaaaa
     2041 gagaacttaa gagagtgtat ttttgtatct ccaattctag atatgcaaca gctcatttca
     2101 aatatgatgc tttgggcaaa tgtatcagaa gaacagctta aacatgaact aattatccca
     2161 acttcatttg ggcatactct ttcatgggaa tatctacaat atgtaaaaca acatcctatc
     2221 actcaatgga atgtaccaac aaaaatatta tatggtaaac atgatgaact tactgaaatc
     2281 ggtgttgtag agaaatttgt agaaaagttt aattgcaacc ttactattat agaaaatggc
     2341 aaacattggt ttaacacacc acaagaatta aattcattat atgaatggtt aaatacttta
     2401 gttaaataaa aaataatta
//
LOCUS       DAMJVK010000213         2409 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.218, whole genome shotgun sequence.
ACCESSION   DAMJVK010000213 DAMJVK010000000
VERSION     DAMJVK010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.218"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            51..968
                     /locus_tag="PC414_003805"
     CDS             51..968
                     /locus_tag="PC414_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2466150.1"
                     /translation="MENNKCREDFRFTQEYEEDYPNTNERYYENYQVADRYYNYPNKY
                     KEPKIKQCCCKKSMREALELLRYDALRPFVNFNQFAFISDFFIVGANLVGIDLSAPPK
                     DNLSGLDGTFERFSACNCDLIDIAGRVSYPIPVPLTLEGLINTIGTIPGVAELIALID
                     AVIPPTIDLGAILDAILAAIIDFILAASTPLANVDLASLCNLKAVAFDITPADYEDFI
                     ASLGYYLDKKHYKECNCNCDCDDCCCNKGILDNLYMSNINNQVTVVAGSLVLTGVEVL
                     GKKNDVIVLGNSNDSRIYFVCVDSIDYIA"
     gene            complement(1110..2291)
                     /locus_tag="PC414_003806"
     CDS             complement(1110..2291)
                     /locus_tag="PC414_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDF2466151.1"
                     /translation="MNKKYIKAGNLIDGTGNSIMQNKGIIIDGDTIVEIEDIRDDLNG
                     YDVYDYLDKTVMPGIMNCHVHLTMEPVGNPKIYYDNVSDVELVVNTIKQLDAYLDSGV
                     TYIRSLGCPKYVDVQLKNLIDKGSIDGPGIVTSGPVICMTGGHGYYFGIESDGVDECR
                     KSARTVLKNGVDCVKIMATGGVTTDGVEPGSPQLTLEEMKAAVDEAIKAGKITATHAQ
                     GRTGIKNAVLAGITSIEHGVYLDDEIIDLMLERGTYLVPTVAAPYFILNAGKESGITE
                     ATLRKCEIVAIPHKESFLKAYKKGIKIAVGSDAGTSYNEHGKTYYEMKLMSDYGMDNM
                     DIIVAATKTASELLRIDKNYGTLEKGKKADVIVVNGNPVENIDVLADVLAVFKLGKKV
                     R"
BASE COUNT          782 a          405 c          309 g          913 t
ORIGIN      
        1 aagcttaatt atctatacat ttaaattaaa atataaaagg agtgtattta gtggaaaata
       61 ataaatgtag agaggacttt agatttacac aagaatatga ggaagattat ccaaatacaa
      121 atgaaagata ctatgaaaat tatcaagtag ctgatagata ctataattat ccaaataaat
      181 ataaagaacc taaaataaaa caatgttgtt gtaaaaaaag tatgagagaa gcattagaac
      241 ttctaagata tgatgctcta agaccttttg taaactttaa tcaatttgct tttatctcag
      301 atttctttat agtaggtgct aatttggtag gtatagatct ttcagctcct ccaaaagata
      361 atttatctgg acttgatggt acttttgaaa gattttctgc ttgtaactgt gatttaatag
      421 atatagctgg tagagtatct tatcctattc cagtcccttt aactcttgag ggattaatta
      481 atactatagg aactatacca ggagtagctg aattaattgc acttattgat gcagttattc
      541 ctcctacgat agaccttggg gctatattag atgcaatact tgctgctata attgatttta
      601 tacttgctgc atctactcca ttagcaaacg tagatttagc atcattgtgt aatcttaaag
      661 ctgttgcatt tgatattaca cctgcagatt atgaagattt catagcatct ttaggttact
      721 atcttgataa aaaacattac aaagaatgta attgcaactg cgattgtgat gattgctgtt
      781 gtaataaagg tatcctagat aatctttata tgtcaaatat aaataatcaa gttactgtag
      841 tagctggtag tttggttcta actggtgttg aagttctagg taagaaaaat gatgttatag
      901 tacttggaaa ttctaatgat tcaagaatat actttgtatg tgtagattct atagattata
      961 ttgcataaga atgattttaa agagttatat ctaaatataa aatagaatct attttctaaa
     1021 gtataaaaga tttaattatt actaataaaa aagagtgtct ataattattt aaaaatttct
     1081 ttagacactc ttttttattt ttatactatt tatctaactt ttttaccaag tttaaatact
     1141 gctaacacat ctgcaagtac gtctatattc tctactggat ttccatttac aactataaca
     1201 tctgctttct ttcctttttc tagagttcca taatttttat caattcttaa aagttcagat
     1261 gctgtttttg tagcagcaac tattatatcc atattatcca taccataatc tgacattagt
     1321 ttcatttcat aatatgtttt tccatgttca ttataagaag ttcctgcatc agaaccaact
     1381 gcaattttaa ttcctttttt atatgctttt aggaatgact ctttgtgtgg aatggcaaca
     1441 atctcacact ttctaagagt agcttcagtt attccacttt cttttcctgc atttagaata
     1501 aaatatggtg cagccacagt tggaactaag taagttcctc tttcaagcat caaatcaatt
     1561 atttcatcat ctaaataaac accatgttct atagaagtaa ttcctgcaag cactgcattt
     1621 tttataccag ttcttccttg tgcatgtgtg gcagttattt tacctgcttt tatagcttca
     1681 tctacagcag ccttcatttc ctccaaagtt aattgtggtg accctggctc aactccatca
     1741 gttgttacac ctccagttgc cattatcttt acacaatcta ctccattttt taaaactgtt
     1801 ctggctgatt ttctacattc atctactcca tcactttcaa taccaaaata atatccatgc
     1861 cctcctgtca tacatataac tggacctgaa gtaactatac caggaccatc tatagaccct
     1921 ttgtctataa gatttttaag ttgtacatcc acatatttgg gacaacctaa gcttctaata
     1981 tatgttactc ctgaatccaa ataagcatca agttgtttta tcgtatttac tacaagctct
     2041 acatctgata cattatcata atatatcttt ggatttccaa ctggctccat tgtcaagtga
     2101 acatgacaat tcataatgcc tggcataaca gttttatcta agtaatcgta cacatcatat
     2161 ccatttaaat catctcttat atcttcaatt tcaactatag tatctccatc aataattata
     2221 cccttatttt gcataatact atttccggtt ccatcaatta aattccctgc tttaatatat
     2281 tttttgttca tcttaaattt ctcccccttt taagtacaat gtattattta tttattattt
     2341 ctaatattct aataattaat tttaccacta taagcattct atttcaaatt taagtgcatt
     2401 aatataaat
//
LOCUS       DAMJVK010000214         2359 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.219, whole genome shotgun sequence.
ACCESSION   DAMJVK010000214 DAMJVK010000000
VERSION     DAMJVK010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.219"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(141..1433)
                     /gene="brnQ"
                     /locus_tag="PC414_003807"
     CDS             complement(141..1433)
                     /gene="brnQ"
                     /locus_tag="PC414_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDF2466152.1"
                     /translation="MGEKQINFKDVIVIGFALFAMFFGAGNLIFPPYLGVLSGSSWLV
                     AFIGFLFADGGLALLAVIAATKFNGDTSKMFSRAGKGLSIALGCAMVICIGPLLAIPR
                     TAATTYEMGILPTIGSGISPVIFSIVFFALVLVLTIRPSKVVDIVGSILTPALLIALA
                     VLIVKGIVSPLGEIRDSSLIQNVFAEGITQGYQTMDALAASVFASIIIMSVIAKGYTG
                     EKEKMKATISAGVIAVIGMALVYGGLCYLGATVSQQYGQDVQQTALIVAITAALLGTT
                     GKILLAIIVALACLTTAIGLSSAAGQYFSTLTDGKLKYEHIVIVVCVFSAIISNFGVS
                     TIIQFSSPILSMVYPATITLVILALFSNKIKNDNVFKCAAYMALLVSVLTVATSFGVK
                     IPLVNSLPFASLGFNWIVPVVIAGIIGNFIPSKSSKTA"
BASE COUNT          795 a          382 c          251 g          931 t
ORIGIN      
        1 caaaaataaa tttttaaatt tatcaaacag gattaataat gttcttttat ctacaataat
       61 ttaataattc ttttaaatat aaaaaggaat tgcctatata atttaaaggc aattccataa
      121 ttatcaatta gtttttatta ttatgcagtt ttactgcttt tagatggaat aaagtttcct
      181 attattcctg ctattacaac tggaactatc cagttaaatc ctaatgaagc aaatggtaag
      241 ctattaacta gaggaatttt tacaccaaaa cttgttgcaa ctgttaatac acttactaat
      301 aatgccatgt atgcagcaca tttgaacacg ttgtcatttt taattttatt actaaataat
      361 gctagtataa ctagagttat agttgctgga tataccatac ttagtatagg agaagagaat
      421 tgtattattg tacttactcc aaagtttgat atgattgcac taaatacaca aactactata
      481 actatatgct catactttaa ttttccatct gtaagtgttg agaaatattg accagctgct
      541 gaagaaagac ctatagctgt tgttaaacat gctaatgcaa ctataatagc caataaaatt
      601 ttacctgtag ttccaagtaa agctgctgtt attgcaacta ttaaagctgt ttgttgaaca
      661 tcttgtccat attgttgaga cacagttgct ccaagataac ataatccacc ataaactaat
      721 gccattccta ttactgcaat tacacctgca cttattgtgg ctttcatctt ttctttctca
      781 cctgtataac ctttagctat tactgacatg attataattg atgcaaatac actagctgcc
      841 aatgcatcca ttgtttgata accttgagtt ataccttctg caaatacatt ttgtattaaa
      901 gaactgtctc ttatttctcc taatggagat acgatacctt taactattaa tactgctaaa
      961 gctataagta aagctggagt taatattgac ccaactatat ctacaacttt tgatggtctt
     1021 attgttaaaa ccaatactag agcaaagaat actatagaga atattactgg gcttattcca
     1081 gaacctattg taggtaatat acccatttca taagttgttg ctgctgttct aggtattgct
     1141 aaaagtggcc ctatacagat taccattgca catcctaatg caatacttaa tcctttgcct
     1201 gcccttgaga acatcttcga tgtgtctcca ttgaacttag ttgctgctat aactgctaat
     1261 aatgccaaac caccatctgc aaaaaggaat cctatgaaag ctactagcca actacttcca
     1321 gataaaactc ctagatatgg agggaagatt aagttccctg caccaaaaaa cattgcaaat
     1381 agtgcaaatc caattacaat tacatcttta aaattgattt gtttctcacc catttaaagc
     1441 acccccattt gaattttact accttttact aaaaaataag tccaatctct taattaaatg
     1501 aaaacctttt gacgagattt tatttaatta tacatttttg acttaaattt gtcaattatg
     1561 tttcataatt tataaaatct tcacaatatt taatacaact ttcttaatta aacaaaatca
     1621 acctgtatac tttaaacata gttggttttt tgttttttct ctaatttttc ttgataaaat
     1681 taactattcc gaataattaa aatattaaga ctttttagat tattcgattt attattaagt
     1741 tgtattgctt tatatactgt atatatcact ctcctttttt acattcatat aatcttttta
     1801 attcaattta aattttaaat attttgtgtt tattatataa tctgaaaaaa aagcagtcaa
     1861 tagcattttt aattttttta attttcaaaa ttatgattct taaaaaatcg ttttcctttt
     1921 atcataacac ctatttacgt taattcttgt agtattttgt atttattttg ctaaattgac
     1981 agttaacttt attcaatttt tattctactt gcgtagattt gcctcttgtt ttttactctt
     2041 aaaaccattt tttctgaaaa ttccttatta tgatttatca acttattttt cttcataaca
     2101 attactctct aacttccaac ttacttaaac tttgcagttg ctagtattca cgtttacata
     2161 cttatatgtc ataattaact aaaaattctt aatatatctc tcaatgaaaa caatttactc
     2221 atttatttaa tacatttata taatcatatg tcttaaattt tgttttatta ttttattatt
     2281 gtcacaattt tatttatatt atatttttgt aaaaatcatt taatatgtat acttaaattt
     2341 ataaaattac tctaaaatt
//
LOCUS       DAMJVK010000215         2127 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.220, whole genome shotgun sequence.
ACCESSION   DAMJVK010000215 DAMJVK010000000
VERSION     DAMJVK010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.220"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            44..>2127
                     /locus_tag="PC414_003808"
     CDS             44..>2127
                     /locus_tag="PC414_003808"
                     /inference="COORDINATES: protein motif:HMM:NF014442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HDF2466153.1"
                     /translation="MEIDLDLYDYKQRRLILEKWRDIIDNKSIDKLNEIQLKEIFVSD
                     ILNKVLRYKSLTDNIMDYNIKPEEKTKIDGTRADIVLGYFSNEESDYRVAIELKKPTT
                     NLDAKQLRKNNHNTPVEQGFSYLPKYGRNCTWLIVSNFKEIRLYNANDATEYEFFSIK
                     DIYEKDDVFKKFLYLLSSKSLMTKHGKSRVEMLWEENIKQEEAIEKGFYDLYKNTRLN
                     LFNDILLNNKDILDVKTCLTKTQKILDRVIFVCFAEHKGLIPENIFETCIQVGKKSVS
                     RFGVWEQVKGLFESIDLGNQEKGINQFNGGLFAEDYILNNLKIPNKCFENLYKLSLYD
                     FNSDLNENILGHIFEKSISDLEELKLEINQVNIKKKDSKRNKDGIFYTPKYITKHIVN
                     NTIRRYLDEKKKELGEDKLPELKEEDYETVVVGRNIQHKRVQVVEYKTDKIQKYINFW
                     KNYRDVVKNIKILDPACGSGAFLNEAFSMLKKEAESVNEILRDLTDGEISFFDLDANI
                     LKNNLYGVDLNEESVEITKLSLWLKTANKYSLLTSLDNNIKCGNSIIDNKEVDCAKAF
                     DWNVEFEDVMMSGGFDIIIGNPPYVSTKMIPEKHREYYWKKYKDLLISEMDLYELFLY
                     EFCENKLKQDGFLGFITPNTYFTNKSFENLRKYLLKNVCVDTILDFPYRFFPFEDVNK
                     ETAIIIINKKLP"
BASE COUNT          897 a          194 c          351 g          685 t
ORIGIN      
        1 aaagtatctt tttaatcaaa aactattaaa agaaaaatat aatatggaaa tagacctaga
       61 cttatatgac tataaacaaa gacgattaat tttagaaaaa tggagagata taattgataa
      121 taaatctatt gataaattaa atgaaattca acttaaggaa atatttgtat cggatatttt
      181 aaacaaagtt cttaggtata aaagtcttac ggacaatata atggattata atataaaacc
      241 agaagaaaaa actaagattg atggaacaag agctgatatt gtactaggct atttttctaa
      301 tgaagaaagt gactatagag tagcaataga attaaaaaaa ccaacaacta atttagatgc
      361 aaaacaacta agaaaaaaca atcataatac accagtagaa caagggtttt cttacttgcc
      421 taaatatgga agaaattgca catggcttat tgtctctaac ttcaaggaaa ttagattgta
      481 taatgctaat gatgctacag agtatgaatt tttttctatt aaagatatat atgaaaaaga
      541 tgatgtattt aaaaaatttt tatatctttt gtcatctaaa tctttaatga ccaaacatgg
      601 aaaatctaga gttgagatgc tttgggaaga gaatataaag caagaggaag ccatagaaaa
      661 aggcttttat gacctatata aaaatactag attaaattta tttaatgata tacttttaaa
      721 taataaagat attttggatg taaaaacttg tttaacaaag acacaaaaaa tattagatag
      781 agttatattt gtttgttttg cagaacataa aggattaata ccagagaata tctttgaaac
      841 atgtattcaa gtagggaaaa aaagtgtatc taggtttgga gtctgggaac aagtaaaggg
      901 tctgtttgaa tctatagatt taggtaatca ggaaaagggc ataaatcaat ttaatggagg
      961 tttatttgca gaagattata ttttgaataa tctaaagata ccaaataaat gtttcgagaa
     1021 tttatacaag ttatctttat atgattttaa tagtgattta aatgaaaata tattagggca
     1081 tatatttgaa aaatctatat ctgacttaga agaactaaaa ctagaaataa atcaagttaa
     1141 tattaagaaa aaagatagta agagaaacaa agatgggata ttttatactc ccaaatatat
     1201 aacgaaacat attgttaata acaccataag aagatattta gatgagaaga aaaaagaatt
     1261 aggcgaagat aaacttccag aactaaaaga agaagattat gaaacagttg tggttggtag
     1321 gaatatacag cataagcgtg tacaagttgt agaatataaa actgataaaa ttcaaaaata
     1381 tataaacttt tggaaaaatt atagagacgt tgtaaaaaat ataaaaattt tagacccagc
     1441 ttgtggaagt ggagcttttt tgaatgaggc tttttctatg ttaaaaaaag aagctgaatc
     1501 agtaaacgaa atacttcgtg atttaaccga tggtgagata tcattttttg acttagatgc
     1561 taatatatta aaaaataatt tatatggagt tgatttgaat gaagaatctg tggaaataac
     1621 taaattgtct ttatggctta aaacagctaa caagtatagt ttattaacta gcttagataa
     1681 taatataaaa tgtggaaatt ctattattga taacaaagag gttgattgtg ctaaagcttt
     1741 tgattggaat gttgaatttg aagatgtaat gatgtcagga gggtttgata ttataattgg
     1801 aaatccccca tatgtttcaa ctaaaatgat tccagaaaag catagagaat attattggaa
     1861 aaaatataaa gatttactaa ttagcgaaat ggatttgtat gagttgtttt tatatgagtt
     1921 ttgtgaaaat aaattaaagc aagatggatt tttgggattt ataacaccta atacgtactt
     1981 tacaaataaa agctttgaaa atttaagaaa atatttactt aaaaatgtat gtgttgatac
     2041 tattttagac tttccatata gattttttcc ttttgaggat gtaaacaagg aaaccgctat
     2101 aattataatt aataaaaaat tgccgaa
//
LOCUS       DAMJVK010000216         2005 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.221, whole genome shotgun sequence.
ACCESSION   DAMJVK010000216 DAMJVK010000000
VERSION     DAMJVK010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.221"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(67..1284)
                     /locus_tag="PC414_003809"
     CDS             complement(67..1284)
                     /locus_tag="PC414_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HDF2466154.1"
                     /translation="MLGKKCMDYLQTLGKISSTTNGLTRLILTQEHKKSIDLISSWME
                     GLNLDIEIDDIGNVIGTYKSSFPNAPTLVVASHQDSVKCGGIFDGMLGIIVPLVGLEE
                     AKHNNRSYPFNIKLIAFAEEEGTRFETSLMGSKVFAGTFKEELLKSVDENGITLEEAV
                     TKFGFNTKNLTNLHPRKDVDAYLEFHIEQGPVLENESLPAGIVSSITGFKSFKISVNG
                     KSGHAGTLPMNMRLDAGCCACECVLAIEKVAKTTTDLVATVGKMNFYPSSSNVVPERA
                     EFTLDVRSCSQEILDNSVEKIFNEISHICENRKLNYTSELAFENVPVPCSNKITKIIE
                     KSFIDLNLNPFYIYSGAGHDAQEMDNITDIGMVFIRCAGGVSHNPNESVSVDDLDTAV
                     KIFLKILDNLDLK"
     gene            complement(1475..1888)
                     /locus_tag="PC414_003810"
     CDS             complement(1475..1888)
                     /locus_tag="PC414_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2466155.1"
                     /translation="MELEITEKIEKQDEDTIFQGLLKYNLARLEDKNPVDLGIYIRDK
                     AGKIVAGFIGVTHGNWLSIKYLWVSEKLRYKGTGSQLLYKAEKIAKERGCKYVFLDTF
                     SFQAPKFYEKFGYHKVFSLENYPLTGKRYYYRKIL"
BASE COUNT          689 a          325 c          227 g          764 t
ORIGIN      
        1 ttatataata taaatataaa gtgatttttt agattatata ttttaatata ctttcttata
       61 taagaattat tttaaatcaa gattatctag tatttttaaa aatattttta cagcagtatc
      121 caagtcatct acagagacac tttcattagg attatgactt acgcccccag cgcatcttat
      181 aaataccata ccaatatcag ttatattatc catttcctga gcatcatggc cagcaccact
      241 ataaatataa aaaggattaa gatttaaatc tataaaactt ttttctatga tttttgttat
      301 tttattagaa catggtacag gaacattttc aaaagcaagt tcacttgtat aattaagttt
      361 tctattttca catatatgtg atatttcatt aaatatcttt tctacagaat tatcaagaat
      421 ttcttgtgaa caagagcgta catcgagtgt aaattcagct ctttcaggta ccacatttga
      481 tgaagatgga tagaaattca tctttcctac agtagcaacc aaatcagtag tcgttttagc
      541 tactttttcg atagctaata cacattcaca ggcacaacat ccagcatcaa gtctcatatt
      601 cattggaagt gtaccagcat gtccagactt accatttact gatatcttaa aacttttaaa
      661 tcctgtaata cttgatacaa ttccagcagg aagagattca ttttctaaaa caggaccctg
      721 ctcaatatga aattctaaat atgcatctac atcttttcta ggatgcaaat ttgttaagtt
      781 ttttgtatta aatccaaact ttgttacagc ttcttcaagt gtaattccat tttcatcaac
      841 agatttaaga agttcctctt taaaagtacc agcaaaaact ttacttccca ttaaagaagt
      901 ttcaaatcta gttccttcct cttcagcgaa agcaattagc ttaatattga aaggataaga
      961 cctattattg tgttttgctt cttcaagacc aactagaggc actattatac ctaacatacc
     1021 atcaaatata cctccacact ttacactatc ttgatgagat gcaactacta atgtgggagc
     1081 atttggaaag cttgatttat atgtacctat aacgtttcca atgtcatcaa tttcaatatc
     1141 aagatttaaa ccttccatcc atgaactaat taaatcaata gattttttat gttcttgagt
     1201 caagataagt cttgtaagac catttgttgt tgaacttatt tttcctaatg tttgtagata
     1261 atccatacat ttttttccta acatatttac ctcctaaaaa tataattttt atttaagcta
     1321 agattagctt ttatacttat aatttttgaa ctttcaaata attttgtatg tatagtaaaa
     1381 tactatttta gtattaataa tgatataaaa acataaagtc tgtagacgaa gagcagaagt
     1441 tttaatttaa aaatatgaaa tatgattttt attattatag tatttttcta taataatatc
     1501 tctttccagt tagaggataa ttttcaagtg aaaatacttt atggtatcca aatttctcat
     1561 aaaatttagg tgcttgaaaa ctgaatgtat ctaaaaaaac atatttacaa cctcgttctt
     1621 tagcaatttt ttcagcctta tataaaagtt gacttccagt acctttatat ctaagcttct
     1681 cactaaccca taagtatttt atagaaagcc aattaccatg agtaacacct ataaaacctg
     1741 caacaatttt acctgcttta tctcgaatat atattcctaa atccactggg tttttatctt
     1801 caagtctagc aagattatat tttaataatc cttgaaaaat agtatcttca tcttgttttt
     1861 ctattttttc tgtaatttct aattccatat ttgaactttc tccttgtata atcttttgta
     1921 tattggtatt ttttaataaa atcagtagaa agaatatagt ttaacattat tctaataggc
     1981 atttatgcta atgtcaaact aataa
//
LOCUS       DAMJVK010000217         1951 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.222, whole genome shotgun sequence.
ACCESSION   DAMJVK010000217 DAMJVK010000000
VERSION     DAMJVK010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.222"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            222..791
                     /gene="mocA"
                     /locus_tag="PC414_003811"
     CDS             222..791
                     /gene="mocA"
                     /locus_tag="PC414_003811"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HDF2466156.1"
                     /translation="MINAVIMASGFARRMGANKLLLNYKGCSIIEHVFREVSKIDFHQ
                     VVVVSQYEEVLLLCNKYGFEYIDNKNANIGQSESIKLGVLNSSKCDGYMFFVGDQPFI
                     DNLYIDKIINTFKLDRDFIVIPRYKDMCGNPVIFPYSKKEELLALKEDEKGKTILKNS
                     SKIKYVEVPKEMLFDIDTKMDYERIGGKL"
     gene            788..1807
                     /locus_tag="PC414_003812"
     CDS             788..1807
                     /locus_tag="PC414_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HDF2466157.1"
                     /translation="MKLIKTIDAVGQVLCHDITQIIPGEFKGRKFKKGHIVKEEDIPV
                     LLSLGKDNLYVWEKSEGMVHENEGALFLKELTAGENLDFSEIKEGKIDFIAACDGLLK
                     IDVDALFDLNCVGEIMMATLHNNFIVNKGLKVAGTRVIPLVIDEKKLEDAKKVVGNRK
                     IVNVVPFKPKKVGIVTTGNEVFYSRIVDKFGPVIEEKVKGFGCEVIGQTICPDDKEII
                     KSAIKEFISQGAELICCTGGMSVDPDDVTPTAIKETGADLVTYGSPILPGAMFLLAYY
                     GEVPIMGIPGCAMYHKTTVFDIVLSRVLIDEKLDKYDIARYGHGGLCMNCDVCTYPAC
                     NFAKI"
BASE COUNT          799 a          167 c          366 g          619 t
ORIGIN      
        1 aaatgaaaaa tataaaaatt tagctataaa attaatagaa aatattaaag aatatgaaag
       61 tgaaatagag atattttatg gaagtgtaaa acaaaaattt tgtgtaagat attgaagtat
      121 taaaatagtt tatatgatat tatatatata gataatatca atagagatat tataaaaatc
      181 atagaactat caatatatta taaataccaa ggatgatata tatgataaat gcagtgataa
      241 tggcatcagg ctttgccaga agaatgggag caaataaact tttattaaat tataaaggct
      301 gttctataat agaacatgta tttagagaag ttagcaagat tgattttcat caagttgtag
      361 tagtaagtca atatgaagag gttttgttat tgtgtaataa atatggtttt gaatatatag
      421 ataataaaaa tgcaaatata ggtcaaagtg aatctataaa attaggagtt ttaaatagct
      481 caaaatgtga tggttatatg ttctttgtag gagaccagcc atttattgat aacttatata
      541 tagataaaat tataaatact tttaaattag atagagattt tatagtaatt ccaagataca
      601 aagatatgtg tggaaatcca gtcatattcc catatagcaa gaaagaagaa ttgttggcac
      661 ttaaagaaga tgaaaaaggt aaaaccattt taaaaaattc atcaaaaata aagtatgtag
      721 aagttcctaa agaaatgtta tttgatatcg atacaaaaat ggactatgag agaattgggg
      781 gtaaattatg aagctaataa aaactataga tgctgttgga caagttttat gtcacgacat
      841 aactcaaata atacctggag aatttaaagg aagaaaattt aaaaagggtc acatagtaaa
      901 agaagaagat attccagtgt tattatctct tggtaaagat aatttgtatg tatgggaaaa
      961 gtcagaagga atggttcatg aaaatgaagg cgcattgttt ttaaaagaac taactgctgg
     1021 agaaaattta gattttagtg aaataaaaga agggaaaata gattttatag cagcatgtga
     1081 tggtctatta aagatagatg ttgatgcact ttttgatttg aattgtgttg gagaaataat
     1141 gatggctact cttcataata attttatagt aaataaaggt ttaaaagtag ctggaacaag
     1201 agtgatacct ttagttatag atgagaaaaa attagaagat gcaaaaaaag tagttggtaa
     1261 tagaaaaata gtaaatgtag taccttttaa acctaagaaa gtaggaattg ttactacagg
     1321 aaatgaagtg ttttatagca gaattgttga taaatttgga ccagtaatag aagaaaaagt
     1381 aaaaggcttt ggttgtgaag ttataggaca gactatatgc ccagatgata aagaaattat
     1441 aaagagtgca ataaaagaat ttataagtca aggagcagaa cttatctgtt gtactggtgg
     1501 gatgagtgta gacccagatg atgttacgcc aacagctata aaagaaactg gagctgattt
     1561 agtgacatat ggttcaccaa tattaccagg agctatgttt ttacttgctt attacggaga
     1621 agttccaatt atgggtatac caggatgcgc aatgtatcac aaaactacag tatttgatat
     1681 agttttatct agggttttaa ttgatgaaaa gcttgataag tatgatatag ctagatatgg
     1741 tcatggtggc ctttgtatga actgtgatgt atgtacttat cctgcatgta attttgctaa
     1801 aatataaagt tattaaaaaa gagtatttac aaattaagta ctctttttaa atatgaaaca
     1861 aaacagctag aatgatgttt taaaaaaagc atatttattt agaaattata ataacttatt
     1921 ttcaatgtgt aaaagaatga ataaagttgc a
//
LOCUS       DAMJVK010000218         1934 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.223, whole genome shotgun sequence.
ACCESSION   DAMJVK010000218 DAMJVK010000000
VERSION     DAMJVK010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.223"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            164..448
                     /locus_tag="PC414_003813"
     CDS             164..448
                     /locus_tag="PC414_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2466158.1"
                     /translation="MLVYNKSFYPNDIFPRLDFSKIKKQLKLIDNDLSDFGSICIIEK
                     EHYTISVNSIGEINVYYDLEYENKVYRIVYEIEKLFKSQVGRFSISTYRN"
     gene            complement(605..778)
                     /locus_tag="PC414_003814"
     CDS             complement(605..778)
                     /locus_tag="PC414_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase"
                     /protein_id="HDF2466159.1"
                     /translation="MKRKLSTYFKGYILGLCIAFLLFTFILKKLIIENIIGIIIGTGI
                     AFLLIYLFNKKRK"
     gene            complement(1219..1434)
                     /locus_tag="PC414_003815"
     CDS             complement(1219..1434)
                     /locus_tag="PC414_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2466160.1"
                     /translation="MAIVVNLDVMMAKRKISLKDLAEKIDITNANLSILKTGKAKAIR
                     FTTLNEICKALDCQPGDILEYVEDEND"
     gene            complement(1445..1912)
                     /locus_tag="PC414_003816"
     CDS             complement(1445..1912)
                     /locus_tag="PC414_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HDF2466161.1"
                     /translation="MITKFFSNILSVFLYFLLSLLGVAGIIFLCMIPMFLYDFSSDKV
                     IALIARILYFSDYFIVILFLIFIINSTKYSPFVLENVKRFKVMGFCLLINTIIECING
                     YNLNTNNIRIFGTDSGGISPLMIISFIFALMCFVIAETFDKAIKIKEDNDLTI"
BASE COUNT          788 a          205 c          236 g          705 t
ORIGIN      
        1 atattttact aatatacatt taaaaattat aatttaaagc attaaaaaat aaatttgtat
       61 tttgttttat ataaagtaat ttgaagaata tggaaaaact tgattagtac attattaatt
      121 agggtataaa atggtgtaaa tagaatgata aggggtggat gaaatgcttg tatataataa
      181 aagtttttat cctaatgaca tatttccaag attagatttt tcaaaaataa aaaaacagtt
      241 aaaattgata gataatgacc tgtcagattt tggaagcata tgtataatag aaaaagaaca
      301 ttatacaata agtgtaaaca gtataggtga aataaatgtg tattatgatt tagagtacga
      361 aaataaggtg tatagaatag tttatgagat tgaaaagtta tttaaatctc aagttggaag
      421 gtttagcata tctacataca gaaattgata attaaaaaag tagaaattaa aaaacttaat
      481 actaaagata tagataaata tatgaggtaa cagctagaga gagttgttac cctttttaaa
      541 tactaagtat atattattct tattaatttt ttgtaaattc actaaaagac ctttttatag
      601 aatattattt tcttttttta ttaaataggt aaatcagtaa aaatgcaatt ccagttccaa
      661 ttattattcc aatgatattt tctataatta atttttttaa tataaaagta aataataaaa
      721 atgctataca taaacctaaa atatatcctt taaaataggt tgaaagtttt cttttcatat
      781 aatcaccaac ttgtatcaga tttagtttac ttaattagag tataacagtg ttgtaacttc
      841 aaagagtatc taaaagatta caaatgttag ataaaatcat caataatgta atttataaca
      901 cgtagctata aactatgcaa taatataaat ggaggtataa gtaaatgaaa aagttccatg
      961 ttttgctaac agtttaaata aatattaggt agaaaagaat gtatatagga atctgtggta
     1021 aaattatcaa tttaaaagta ttatttagtg tgatacagtt cttataggta atggataaca
     1081 aatagataaa ggaacagttt ctttcttaga acatattttg ttatgacttt aaacaaagtt
     1141 aatattaatg tcaataatat agtaaaagaa taaaaatcat atagtataat aaaaaagagt
     1201 gtttctaaac actctttttt aatcattttc atcttctaca tattcaagta tatcacctgg
     1261 ctgacagtct aaagctttgc aaatttcgtt tagagtggta aatctaatag cttttgcttt
     1321 tccagttttt aaaattgata aatttgcatt tgtaatatct attttttctg ccaaatcttt
     1381 aagtgatatt ttcctttttg ccatcattac atctaggtta actactattg ccataacatc
     1441 acctctatat cgttaaatca ttatcttctt ttatttttat agctttatca aatgtttctg
     1501 caattacaaa gcacattagt gcaaatataa aagagataat cataagtggt gagataccgc
     1561 cactatcagt tccaaaaatt cttatattat tagtatttaa attataacca ttgatacatt
     1621 caattatagt gtttatcaat aaacaaaatc ccattacctt aaaccttttt acattttcaa
     1681 gtacgaatgg ggaatactta gtactattaa ttataaatat caagaataag atgactataa
     1741 agtagtcaga aaaatatagt attcttgcaa ttaaagcgat tactttgtca gaactaaaat
     1801 catataaaaa cataggtatc atacataaga atattatacc agcaactcct aaaagtgata
     1861 atagaaaata tagaaatact gataatatat tactaaaaaa ttttgtaatc ataaaatatc
     1921 atctccttta tata
//
LOCUS       DAMJVK010000219         1925 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.224, whole genome shotgun sequence.
ACCESSION   DAMJVK010000219 DAMJVK010000000
VERSION     DAMJVK010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.224"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            119..235
                     /gene="rrf"
                     /locus_tag="PC414_003817"
     rRNA            119..235
                     /gene="rrf"
                     /locus_tag="PC414_003817"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            242..316
                     /locus_tag="PC414_003818"
     tRNA            242..316
                     /locus_tag="PC414_003818"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:274..276,aa:Asn,seq:gtt)
     gene            321..395
                     /locus_tag="PC414_003819"
     tRNA            321..395
                     /locus_tag="PC414_003819"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:354..356,aa:Glu,seq:ttc)
     gene            401..476
                     /locus_tag="PC414_003820"
     tRNA            401..476
                     /locus_tag="PC414_003820"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:434..436,aa:Val,seq:tac)
     gene            482..558
                     /locus_tag="PC414_003821"
     tRNA            482..558
                     /locus_tag="PC414_003821"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:516..518,aa:Asp,seq:gtc)
     gene            569..643
                     /locus_tag="PC414_003822"
     tRNA            569..643
                     /locus_tag="PC414_003822"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:601..603,aa:Thr,seq:tgt)
     gene            658..742
                     /locus_tag="PC414_003823"
     tRNA            658..742
                     /locus_tag="PC414_003823"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:692..694,aa:Tyr,seq:gta)
     gene            752..825
                     /locus_tag="PC414_003824"
     tRNA            752..825
                     /locus_tag="PC414_003824"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:784..786,aa:Gly,seq:tcc)
     gene            836..912
                     /locus_tag="PC414_003825"
     tRNA            836..912
                     /locus_tag="PC414_003825"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:870..872,aa:Arg,seq:tct)
     gene            922..997
                     /locus_tag="PC414_003826"
     tRNA            922..997
                     /locus_tag="PC414_003826"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:955..957,aa:Gln,seq:ttg)
     gene            1009..1084
                     /locus_tag="PC414_003827"
     tRNA            1009..1084
                     /locus_tag="PC414_003827"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1042..1044,aa:Lys,seq:ttt)
     gene            1087..1175
                     /locus_tag="PC414_003828"
     tRNA            1087..1175
                     /locus_tag="PC414_003828"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1121..1123,aa:Ser,seq:tga)
     gene            1193..1283
                     /locus_tag="PC414_003829"
     tRNA            1193..1283
                     /locus_tag="PC414_003829"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1227..1229,aa:Ser,seq:gct)
     gene            1292..1368
                     /locus_tag="PC414_003830"
     tRNA            1292..1368
                     /locus_tag="PC414_003830"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1326..1328,aa:Pro,seq:tgg)
     gene            1454..1529
                     /locus_tag="PC414_003831"
     tRNA            1454..1529
                     /locus_tag="PC414_003831"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1487..1489,aa:Trp,seq:cca)
     gene            1590..1666
                     /locus_tag="PC414_003832"
     tRNA            1590..1666
                     /locus_tag="PC414_003832"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1624..1626,aa:Pro,seq:tgg)
     gene            1673..1749
                     /locus_tag="PC414_003833"
     tRNA            1673..1749
                     /locus_tag="PC414_003833"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1707..1709,aa:Ile,seq:gat)
     gene            1753..1828
                     /locus_tag="PC414_003834"
     tRNA            1753..1828
                     /locus_tag="PC414_003834"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1786..1788,aa:Phe,seq:gaa)
     gene            1836..1912
                     /locus_tag="PC414_003835"
     tRNA            1836..1912
                     /locus_tag="PC414_003835"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1870..1872,aa:Met,seq:cat)
BASE COUNT          484 a          442 c          524 g          475 t
ORIGIN      
        1 attcattaag atatatgtgc agtttttaga gtgttaactc taaaaaaaaa taaaaaaagt
       61 attgacaaaa atagtaaaaa atgttaatat tatacttgtc ctagcaacaa gaaaaaaatg
      121 tggttataat agcggagaag atacacctgt ttccattccg aacacagaag ttaagttctc
      181 tagcgctgat ggtacttggt gggaaactgc ctgggagagt aggacatagc cacgtgaatg
      241 ttccaaggta gctcaacggt ggagcaaccg gctgttaacc ggtaggctgt gggttcgagc
      301 cccacccttg gagccattat ggctcgttgg tcaagaggtt aagacaccgc cctttcacgg
      361 cggtaacagg ggttcaattc ccctacgagt caccatttgt gggatcatag ctcagctggg
      421 agagcacctg ccttacaagc agggggtcac aggttcgagc cctgttggtc ccaccatttg
      481 cggcctggta gttcagttgg ttagaatgcc agcctgtcac gctggaggtc gagggttcga
      541 gccccttcca ggtcgccaaa ataaaaatgc tggtgtggct caacggtaga gcagctgact
      601 tgtaatcagc aggttgtagg ttcgattcct atcaccagct ccatgtaaaa ctaatacgga
      661 ggatttcccg agcggccaaa gggggcagac tgtaaatctg ttgtcatcga cttcggtggt
      721 tcgaatccac catcctccac caattaaata tgcgggtgta gctcaatggt agagttctgg
      781 ccttccaagc cagctgtgag ggttcgatcc ccttcacccg ctccaaagaa agcgtgggtg
      841 catagctcag ctggatagag caacgccctt ctaaggcgtg tgtccggggt tcgaatccct
      901 gtgcgctcac cacatatata ttggggatta gccaagtcgg taaggcacat gactttgact
      961 catgtatgcg taggttcgag tcctgcatcc ccagccaaat aaaaatatgg tctattagct
     1021 cagtcggtag agcacctgac ttttaatcag ggtgtcccgc gttcgagtcg cggatagatc
     1081 accaatggag aggtgtccga gtggtttaag gagctggtct tgaaaaccag tgacttcgaa
     1141 aggggccgtg ggttcgaatc ccaccctctc cgccaaaaaa atgactgaat ttggagaagt
     1201 actcaagtgg ctcaagagga tcccctgcta agggattagg ctgggtaacc ggtgcgaggg
     1261 ttcgaatccc tccttctccg ccattcaaaa acgaggtgta gcgcagtttg gtagcgcaca
     1321 tggtttggga ccatggggcc gggggttcga gtcccttcac ctcgaccaac ttaagctttt
     1381 agttcagttg gttagagtgc ccaactcatg gctagtaatt ctagggtttg agtccatgaa
     1441 ggttattctt cataggggtg tagctcagtt ggtagagcgt cggtctccaa aaccgtgcgc
     1501 cgggggttcg agtccctcca cccctgccaa ataaaaagga cttgaaaaaa gttggatttt
     1561 agagtagtat aagatagtaa ataagatttc gaggtgtagc gcagtttggt agcgcacatg
     1621 gtttgggacc atggggccgg gggttcgagt cccttcacct cgaccaagaa aagggcctat
     1681 agctcaggtg gttagagcgc acgcctgata agcgtgaggt cgctggttcg agtccagcta
     1741 ggcccaccat ttgcccagat agctcagtcg gtagagcagg ggactgaaaa tccccgtgtc
     1801 ggtggttcga ttccgcctct gggcaccagg aatacggcgg tatagcttag ttggctagag
     1861 cgttcggttc atacccgaaa ggtcataggt tcgactccta ttaccgctac catctaacta
     1921 tatca
//
LOCUS       DAMJVK010000220         1806 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.225, whole genome shotgun sequence.
ACCESSION   DAMJVK010000220 DAMJVK010000000
VERSION     DAMJVK010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.225"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            116..1714
                     /locus_tag="PC414_003836"
     CDS             116..1714
                     /locus_tag="PC414_003836"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HDF2466162.1"
                     /translation="MSLLVFGHKNPDTDSICSAISLAHLKNKLGIKATAYALGDIRKE
                     AKYALDYFKVDAPEILDNVRIQVRDLNYDKVVPLTPTSSILEAYNLMDEKNVKTLPVT
                     YDDGTFAGIITMKEIAKNLLHQHFTTIHTSLSNICKNLNGTILVDCGEDIKGRVVTLS
                     FGMETLIETLKEGDIAIVGDRYDSIEYAIDTKVKLLILTNHTEISKDLLALAKINGVS
                     VVSVESDNYKTSNVINQCSFLDSIEATENLITFKEDDYMDEIKEIMLETNFRSYPILD
                     DDNKFLGLVSKGHLLNPSKKNVVLVDHNEYAQSADGIEQANIVEIIDHHKLGGISTDV
                     PMSFRVMPVGCNSTIIYQMYKENNVEIPYEIAGLLLSAILSDTLLFKSPTTTDIDKKA
                     CEELSKIAKVDMEKYAMDMFKCGTSLDEYTIEEIVNMDFKEFNMSGHRVGIGQVFTLD
                     IDSIFAKKDEFLSYINSTDYDKLILAVTDIIKEGSYLIYKAEDSLIANAFGVEGVQGT
                     FAPGVVSRKKQLVPNLTTAIKNFK"
BASE COUNT          693 a          221 c          276 g          616 t
ORIGIN      
        1 tttatataat attttttcac taaatttttt tctttttaca acaaatactc actacctttt
       61 gatataatat ttttatcaat tattattata ttcaagtttt gaaaggagta ttaaaatgag
      121 tttattagtt ttcggacata aaaatccaga tacagattca atatgttctg caatttcatt
      181 agcacattta aagaacaaac taggaataaa agcaacagct tatgctttag gagatatcag
      241 aaaagaagct aaatatgcct tggattactt taaggtagat gcacctgaaa ttttagataa
      301 tgttagaata caagttagag atttaaatta tgataaagta gtacctttaa caccaacttc
      361 atctatatta gaggcttata accttatgga tgaaaaaaat gttaaaacac tacctgtaac
      421 ttatgatgat ggaacttttg ctggtatcat tactatgaaa gaaatagcta aaaatctact
      481 tcatcaacac tttacaacta tacacacatc actatcaaac atttgtaaaa accttaatgg
      541 tactattctt gtggactgtg gtgaagatat aaaaggtaga gtagtcactt tatcatttgg
      601 aatggagact ctaatagaga ctttaaaaga aggagacata gctattgttg gagatagata
      661 tgatagtatt gaatatgcta ttgatacaaa agttaaactt ttaatactta ctaatcatac
      721 agagatttca aaagatttgc tagctcttgc taagataaat ggagtgtctg ttgtttctgt
      781 tgaaagtgac aattataaaa cttctaatgt aataaatcaa tgtagtttct tagactctat
      841 agaagctaca gaaaatttaa ttacattcaa agaagatgat tatatggatg aaataaaaga
      901 gattatgcta gaaacaaact tcagatctta tccaatatta gatgatgata ataagttttt
      961 aggtttagtt tcaaaaggtc atctattaaa cccatctaaa aagaatgttg ttttagttga
     1021 ccataacgaa tatgctcaaa gtgctgatgg aattgaacaa gcaaacatag ttgaaataat
     1081 tgaccatcat aaacttggtg gtatttctac tgatgttcct atgtcattta gagttatgcc
     1141 tgttggatgc aatagtacta taatttatca aatgtacaaa gaaaataatg ttgagatacc
     1201 ttatgaaata gccgggcttc ttttatctgc tatattatca gatacattat tatttaagtc
     1261 tccaacaaca actgatatag ataagaaagc ttgtgaagaa ttaagtaaaa tagcaaaagt
     1321 agacatggaa aaatacgcta tggatatgtt taaatgcggt acttctttag atgaatatac
     1381 tatagaagaa atagtaaata tggactttaa agaatttaat atgagtggac atagagttgg
     1441 tattggacaa gtatttacct tagacataga ctctatattt gctaaaaaag atgagttttt
     1501 atcttatata aattcgactg attatgacaa actaattctt gcggttacag atataatcaa
     1561 agaaggttct tatttaatct ataaagcaga agacagctta attgctaacg catttggtgt
     1621 tgaaggtgtt caaggtacat ttgcaccagg tgttgtatca agaaaaaaac aattagtccc
     1681 taatttaact actgcaatca aaaattttaa ataatataaa atttttaaat aacgaaaact
     1741 gtctcaaatc tatatttgag acagtttttt attatatata tatatattga taataaagtt
     1801 aaataa
//
LOCUS       DAMJVK010000221         1802 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.226, whole genome shotgun sequence.
ACCESSION   DAMJVK010000221 DAMJVK010000000
VERSION     DAMJVK010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.226"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            206..1546
                     /locus_tag="PC414_003837"
     CDS             206..1546
                     /locus_tag="PC414_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF2466163.1"
                     /translation="MIKSSLFWNIFIAISFMIEWFLCKKMLDYTSEKRISNKKINFCM
                     VGIIVFADIIYFFDVPPNIRVIISMIITIAFYKISYDVGFIKSVLISLIYWMLLIATD
                     ALSISLTVWLNSINDMSSMGTGDIYRIESIILGKILLIVSLLLYRALKIKLNIEINKR
                     TLFYIVIPIVANIASFFVIFEYIFNPGSGSLIHNFQFVMISVVLLISSITLVLIAKKL
                     QQYNTLVVENNMIKNTLKYENEYYEDIEKKYLKTRLLSHDMKNHILCINAMIKKGIDV
                     TSYLNNLQVEIQSNDLLFNTGNFILDAILSEKKEVCDKNDIELKIGINFSCCEAVELV
                     DICHIFSNILDNAIEACNKIDSGNRVICVKGDVMQNFYLIRVENTKINKIITKNGDIL
                     TDKKGSLHGLGIKSVKNSVNKYGGNVVIEYDDKKFVVKIAIPLRNVKKAVNHVI"
BASE COUNT          726 a          127 c          286 g          663 t
ORIGIN      
        1 aaatttatat agttattatt aaaaaaagca ataaaaagtt taattcaatt tagcaaattt
       61 agtgatataa ttaaaaaaga aaagaaatat ggacttattt ggaataattt tgacaatcta
      121 tttactacat gtgtagagtc gtatataatt aaattataca taaaatataa aaaaataatt
      181 tagtaatacg ttaggagaaa gtattatgat aaagtcaagc ttattttgga atattttcat
      241 tgcgatatcc tttatgatag aatggtttct ttgtaagaaa atgctagatt atacaagtga
      301 gaaaaggatt tctaacaaaa aaataaactt ttgtatggta ggtattattg tttttgctga
      361 cataatatat ttttttgatg tacctccaaa tataagagta attatatcta tgataattac
      421 tattgcattt tataaaatct catatgatgt ggggtttata aagtctgtat taatatcctt
      481 aatttattgg atgttactaa tcgccacaga tgctttgagt atatctttaa cagtttggct
      541 taattctata aatgacatga gtagtatggg gacgggagat atatacagaa ttgagtctat
      601 aatattggga aagatacttt tgattgtgtc tttgctttta tatagagcct taaagataaa
      661 acttaatata gaaataaata aaaggacatt attttatata gttattccta tagtcgctaa
      721 tatagcatct ttttttgtaa tttttgaata catatttaat ccagggtctg gtagcttaat
      781 acataatttc caattcgtta tgatttctgt tgtattgctt atatcaagta tcactttagt
      841 tttaattgct aaaaaattac agcaatataa tactttagtt gtagaaaaca atatgattaa
      901 aaatactttg aagtatgaaa atgagtatta tgaagatata gaaaagaaat atttaaaaac
      961 tagattacta agtcatgata tgaaaaatca catattgtgt ataaatgcta tgattaaaaa
     1021 aggtattgat gttacttctt atttaaataa tttgcaggta gaaattcaga gtaatgactt
     1081 gttatttaat actggtaatt ttatattaga tgctatatta agtgaaaaga aagaggtttg
     1141 tgataagaat gatatagaac taaaaatagg aataaatttt agttgttgtg aagctgttga
     1201 attagtggat atatgtcata tattttcaaa tattttagac aatgcaattg aggcatgtaa
     1261 taaaattgat agtggaaata gagttatttg tgttaaaggt gatgttatgc aaaattttta
     1321 tttaataaga gttgaaaata ctaagataaa taagattatt actaaaaatg gagatatttt
     1381 gacagacaaa aaaggttctc ttcatggact tggaataaaa agtgtaaaaa actctgtaaa
     1441 taagtatggt ggtaatgttg taattgaata tgatgataaa aaatttgtag ttaaaattgc
     1501 tattccactt agaaatgtga agaaagcagt taatcatgtg atttaaatgt atataaattt
     1561 gatgtgtaga aatttaatat ataagaatta atgtataaaa gtgatgcata gaagtttaat
     1621 atataagaat taatgtataa aagtgatgca tagaagttta atatataaga actaatgtat
     1681 aaaagtgatg cataaaagtt taatatataa gaatttaata tgtataaaag tgatgcataa
     1741 aagtttaata tataggaatt taatatgtat aaaagtgatg catagaaatt taatatataa
     1801 ga
//
LOCUS       DAMJVK010000222         1769 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.227, whole genome shotgun sequence.
ACCESSION   DAMJVK010000222 DAMJVK010000000
VERSION     DAMJVK010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.227"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(792..1109)
                     /locus_tag="PC414_003838"
     CDS             complement(792..1109)
                     /locus_tag="PC414_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2466164.1"
                     /translation="MNFRNIYLILMGLGIIILTTIISLIEIKIGFCNEKYFNKLESKY
                     GNIDRKRTIKLEVLYRYVTGFEYIAIGLFTKRLDITIIAIILVATITVILYYLVRKRY
                     ITI"
     gene            complement(1419..1769)
                     /locus_tag="PC414_003839"
                     /pseudo
     CDS             complement(1419..1769)
                     /locus_tag="PC414_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889008.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          633 a          225 c          178 g          733 t
ORIGIN      
        1 tttttggaaa ttatatatat tataacaatt atagcaaatt tttagggtat aatttttaaa
       61 attatattct actatcaaaa gatatgaata aatgattgta tatattatat caatttttat
      121 atttcatagt ccattttttc atcacatttt cgcttgtcaa gaataatatc tttaagagtg
      181 aagcgtttga tgtaaatctg gactttgttt ttgagacatt tttgaaatat ttattaaatc
      241 tttcgattat acttgtaata tatattatag atattactta ttttagtaaa actaaaggat
      301 gatgctacaa cattccagtt atttcccaaa attttaattt ctgaagtata ctgtttacat
      361 tatttttaat ctattttatc taaagtcgat aaggcttctt attcattaac tgacctatat
      421 acagatttaa aattacttat taattctttt gagtccttgt atgaaacata tttaaagtat
      481 ttcttatcta atgtattaaa catttttgta ttacagaatg aggataactt gtagctattg
      541 tctctttgaa ataagtcatc tgtgtttact cctaaaacta cttatatctg tagattttat
      601 aagttctcca tcttatataa tttctttgat taattgtttt cttaaattat tcataaaaaa
      661 atctctgtta caatatttga ttatatttaa atcattgcta taaagaagac ttttattaac
      721 tttgaaaaac aaattatttt aattgctggt gattagttgc taccttcttt tgatattaat
      781 tctattagat tttatatagt tatatatctc tttctaacta agtaatataa tattactgtt
      841 atagtagcta ctaatattat tgctattatt gtaatatcta atctttttgt aaataatcct
      901 attgctatat attcaaaacc tgttacatat cgataaagta cttctagttt aatagttctt
      961 tttctatcta tatttccata ttttgattct aatttattaa aatatttttc attacaaaaa
     1021 ccaattttaa tttcaattaa agatattatt gtagttaata ttataattcc caatcccata
     1081 agtataagat aaatgttcct aaaattcata gaagatacct cctttataat ataatcatca
     1141 actcatgttg tattaatttt cctaatcaaa gtataacagt aacacaagta taaagagtat
     1201 ctaaaatatt acaaatatta aatgagaaaa agatggtaag ttaaccccac catcttattt
     1261 tttatctact aatgaatgat gctacaaaag ttagtataaa aaatgaaaca aacaatataa
     1321 tgaacttgtg taatttactt agttggaatg ctttattaca aaataataaa attaatgctg
     1381 aaacaattgc aaaaagtaga tattttataa tcattttatc acctattgat ttctacagat
     1441 tgtaagtagt atttggttac acaagcacct cctttattat aacgtatttt ttaaatttaa
     1501 agatttttcc agatgttata tttgactcaa ggtacattgt aattaatcta actataagtg
     1561 gcaagtactg tcctatacca ggaatagcac cagccaaaag tcctgaaatc cctataccag
     1621 catcagttaa agttaatgca actcctttag aaagatataa attaaaacct attctagccc
     1681 aaactatttt atttatttca gcttttctat aatataattc aagtagtgtc aatatcgttc
     1741 ttcttaaggt ttttaattag cttatatat
//
LOCUS       DAMJVK010000223         1741 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.228, whole genome shotgun sequence.
ACCESSION   DAMJVK010000223 DAMJVK010000000
VERSION     DAMJVK010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.228"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            122..1450
                     /locus_tag="PC414_003840"
     CDS             122..1450
                     /locus_tag="PC414_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF2466165.1"
                     /translation="MGSNELKKTLGVSAALSTVVGSVIGAGVFFKPQAVYTLTGGAPG
                     LGILAWLIAGIITITAGLTAAEVSVAIPKTGGMMVYIKEIYGEKLGFLTGWMQIVLFY
                     PGMMAALGVIFGEQASALIGSPSLLLPIAIGIIIIVAGLNMLGSKTGGVIQTVSTICK
                     LIPLILIMVVGFIKGGGNNPILTPMVGEGLSLGSVLGQVLIAILFAFDGWMNVGTLAG
                     EMKNPGKDLPKAIIGGLSVVMAVYFIINLAYLWVLPANELANYASPASAVAEVIFGSM
                     GGKIISVGILISVFGALNGFLLTGSRVAYTLATDKTLPKYGIFSKLNSAQVPANAIAL
                     VSVIASIYALSGQFNLLTDLAVFATWIFYVLTFIGVMKLRKTHPNIPREYKVPLYPIV
                     PIIAIASGIFVVVNQLCFAGMKTTMISIGGLVITAIGLPVYAYMTRGVKR"
BASE COUNT          588 a          203 c          323 g          627 t
ORIGIN      
        1 aagctaagta gagtttattt tattttaata aataataaag ctatttttct atatattggc
       61 ataatatatg cttatttaac tatgaaatta aaattaaata ggattaatga ggaggaaaaa
      121 aatgggaagt aatgaactga aaaagactct tggcgtatca gcagcattat caactgttgt
      181 tggttccgtt ataggagcgg gcgtattttt taaaccacaa gcagtctata cattaactgg
      241 aggagctcca ggtcttggta tattggcatg gttaatagct ggaatcataa ctataacagc
      301 tggtcttaca gctgcagagg tatcagttgc gataccaaaa actggtggaa tgatggtata
      361 tataaaagaa atttatggag aaaaattagg ttttttaact ggatggatgc aaattgtatt
      421 attttatcca gggatgatgg cagcattagg tgttatattt ggagaacaag catcggcact
      481 aattggtagt ccatcacttt tacttccaat agcaattgga attataataa tagttgcagg
      541 gcttaatatg ttaggttcaa aaactggagg agtcatacaa actgtttcta caatttgtaa
      601 gttaatacca cttattttaa taatggttgt tggatttatt aaaggtggtg gaaataatcc
      661 aatacttaca cctatggttg gtgaaggatt aagcctgggt agtgtactag gtcaagtttt
      721 aattgctata ttatttgctt ttgatggatg gatgaatgtt ggtactttag ctggagaaat
      781 gaagaatcct ggaaaagacc ttccaaaagc tataataggt ggattgtcag ttgttatggc
      841 tgtttatttt ataataaatt tagcgtatct ttgggtatta ccagctaatg aattagcaaa
      901 ttatgcttct cctgcatcag cagttgctga agttatattt ggttcaatgg gtggaaaaat
      961 aatatctgta ggtatattaa tttcagtgtt tggagcatta aatggatttt tactaactgg
     1021 ttcaagggtg gcatatactt tagctacaga taagacttta ccaaagtatg gtatattttc
     1081 taaattaaat tctgctcaag ttccagcaaa tgcaatagca ttagtatctg ttattgcatc
     1141 tatatatgct ttgtcaggtc aatttaattt attaactgac ttagctgtat ttgctacttg
     1201 gatattctat gtattaactt ttataggggt tatgaaactt agaaagactc atccaaatat
     1261 accaagagag tataaagttc cattatatcc tattgttcct ataatagcta tagcaagtgg
     1321 tatatttgtt gtagttaatc aattgtgttt tgcaggtatg aaaactacta tgatatctat
     1381 aggaggtctt gtaataacag ctataggctt accagtgtat gcttatatga ctagaggagt
     1441 aaaaagataa accaagtagt ttacaaatgc ttcataatta aaataaaaga gactgtctca
     1501 aatagagata agtctctttt ttattgttaa aatggtaaag atatgtttaa ttttacaaaa
     1561 aaagagcgtt ccatgaacta aaaagtttat tttaaggtat tcattttatt tttagttttt
     1621 tgattataaa attaaacatc taaaaaatta tttattttaa aatactttat tttaaagtgt
     1681 tttttatcaa actaagtggt atctgtacaa caaatattat acatagcttt tatgaaaatt
     1741 a
//
LOCUS       DAMJVK010000224         1696 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.229, whole genome shotgun sequence.
ACCESSION   DAMJVK010000224 DAMJVK010000000
VERSION     DAMJVK010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.229"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            180..1631
                     /locus_tag="PC414_003841"
     CDS             180..1631
                     /locus_tag="PC414_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HDF2466166.1"
                     /translation="MDFFNGILTSINNAVVYVNNYLWSYILIIMLVVIGIYFTIKTNF
                     VQFRYFIEMFRLLGDGTANKNAKKEGKISSFQAFCISTSSRVGTGNIAGIAIAVVAGG
                     PGAVFWMWLIALIGSASSFVESTLAQIYKVKDGQAFRGGPAYYMEQGLNKKWMGILFS
                     ILITICYGFVFNAVQANTVSLAFNNAFGISKMAMGIGLGILTAIVIFGGVHRVAKVSE
                     IIVPVFAGLYILVALAIVVMNITEIPSVIALIVESAFDFKGMAIGTFMGVVMTGVKRG
                     LFSNEAGMGSAPNAAATAHVTHPVKQGLIQSLGVFTDTIIICSCTAFMVLLYSGYSSS
                     GATGIELAQEALTHHIGPVGNIFIAVCIFLFAFSSIVGNYYYGESNMEFMSGSKTKLN
                     VFRVFVVGMVLFGSLTQVDVVWNLADLFMALMAIINLIAIALLGKYAFIALKDYTSQK
                     KSGIKDPVFIADEIEGLEKVSEWHRELSEEHLG"
BASE COUNT          586 a          167 c          333 g          610 t
ORIGIN      
        1 aaaggtttga tttgaatata aaactgcaat atattaaaaa aattattcat tttaaacctt
       61 gtaatttgat tttataaata ttataattaa attaatgaat ttttaacaag gaattattca
      121 tttttttgat tttaagattt attgagaaaa caaaactaaa taggggggta attaatataa
      181 tggatttttt caatggtatt ttaacgagta ttaacaatgc agtagtatat gtaaacaatt
      241 acttatggtc gtatatctta attataatgt tagttgtaat tggtatttac tttactatta
      301 aaacaaattt tgtacaattc agatatttta tagaaatgtt tagacttctg ggtgatggta
      361 cagcgaataa aaatgcaaaa aaggaaggaa aaatctcttc atttcaagca ttttgtataa
      421 gtacatcttc aagagttgga actggaaata tagctggaat agcaattgca gtggtagcag
      481 gaggacctgg tgctgtattt tggatgtggc ttatagcatt aattggttct gcatcaagct
      541 ttgttgagag tacattagct cagatttaca aagtgaaaga tggtcaagct tttagaggtg
      601 gaccagcgta ttatatggaa caaggtctaa ataaaaaatg gatgggtatt ttattctcaa
      661 tacttataac tatatgttat ggttttgtat ttaatgcagt tcaagcaaat actgtttcac
      721 ttgcatttaa taatgcattt ggaataagta aaatggctat gggaataggt cttggaattt
      781 taactgcaat agttattttt ggtggagtgc atagagttgc aaaagtttct gaaataatag
      841 ttcctgtatt tgcaggatta tatatattag ttgctttggc tatagttgta atgaatataa
      901 cagagatacc aagtgttata gcattgatag ttgaaagtgc atttgatttt aaaggaatgg
      961 cgattggtac atttatggga gttgtaatga ctggagtgaa gagaggtcta ttttctaatg
     1021 aagcaggtat gggtagtgct cctaatgctg ctgcaacagc acatgtaact catcctgtaa
     1081 aacaaggttt aattcaatct ttaggtgttt ttacagatac tataataata tgtagttgta
     1141 cagcattcat ggtattatta tattcaggtt atagtagtag tggagcaact ggaattgaat
     1201 tagctcaaga agctttaact catcatatag gaccagttgg aaatatattt atagcagttt
     1261 gtatattctt gtttgcattt agttctatag ttggtaatta ctattatggt gaatcaaata
     1321 tggagtttat gagtggaagt aagactaaac ttaatgtatt tagagttttt gttgtaggta
     1381 tggttttatt tggttcactt actcaagtgg atgtagtttg gaatcttgca gacttattta
     1441 tggcattgat ggcgataata aacttaatag caatagcact tttaggaaaa tatgcattta
     1501 ttgctttaaa agattataca agtcaaaaaa aatcaggaat aaaagaccct gtgtttattg
     1561 cagatgaaat agaaggccta gaaaaggtat cagagtggca tagggaatta tctgaggaac
     1621 atttaggata aaaaatagta taaaattata aaaccatttt gaaagactag agtatgtata
     1681 tactttatat gaatat
//
LOCUS       DAMJVK010000225         1676 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.230, whole genome shotgun sequence.
ACCESSION   DAMJVK010000225 DAMJVK010000000
VERSION     DAMJVK010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.230"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(141..1532)
                     /locus_tag="PC414_003842"
     CDS             complement(141..1532)
                     /locus_tag="PC414_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HDF2466167.1"
                     /translation="MSSTESVSKKSGHPKSFWLICMTILWERFSYYGLTALLVLFFTA
                     SVAQGGLGLSTQEATSMFGLFTGLIYLTPLVGGWLADKVLGQQKCISIGALLIAIGDF
                     VLFASSTSIAMVWVGLSIIILGNGFFKSSCSNIVGDIYPKTDVSRKDAAYSLFYMAVN
                     VGAFIAPIICGLLSDNVFAVRGASGDIVSYGYKMAFLICGIGMMLGMIIFTLLAPKLL
                     NEVGKYPVTKGNKGEKIEYGPLTKLEKNRIIAMVILFLFVVLFWTAYYQTQSSFMIYV
                     RDNVNRTLFGFEMPVAWLTSLNAILCVALCPVLATLWVKLSHTKRGDFSIPVKMGLGM
                     IIMGVGFIVMVFATLANGPDGMVKASILFIVLTYIFTTVGELFLSPIGLAMFNKLAPA
                     KYATLSMGAWYLTSFFASLLSGKIAGFTATLGFLQIFGGIATVLIIFGLILLGIRNVL
                     TRLMAMDLLTKEK"
BASE COUNT          601 a          311 c          183 g          581 t
ORIGIN      
        1 acaattataa ttttaattat aatatctatt tgtgtaaagc tttttttcat aatcaaagag
       61 gaaattacat ttaattttat atttaaatgt aacttccttt atttaaagta attcatttat
      121 ttacaataat tcgtcatgaa ttatttttct tttgttagta aatccatagc cattaatctt
      181 gttaatacat ttcttattcc caataatatt aaaccaaata ttattaaaac tgttgctatt
      241 cctccaaata tctgtaaaaa accaagtgtg gctgtaaaac cagcaatctt tcctgataat
      301 aaacttgcaa aaaaacttgt tagataccag gctcccatag acaacgtagc atacttagct
      361 ggagctaatt tattgaacat tgcaagtcct ataggagata agaataattc acctactgtt
      421 gtaaatatat aagtcaatac tataaacaat atactagcct ttaccattcc atctggacca
      481 ttagctaatg tagcaaatac cataactata aacccaacac ccattattat cattcctaat
      541 cccattttaa ctggaataga gaaatcacct cttttggtat gagataattt aacccatagg
      601 gtagctaata ctgggcataa agccacacat aatatagcat ttagtgatgt aagccatgct
      661 actggcattt caaatccaaa tagagttcta tttacattat cccttacata aatcataaaa
      721 gaactttgtg tttgatagta tgctgtccag aataagacca caaataaaaa taaaataacc
      781 attgctatta tcctattttt ctccaactta gtaagtggtc catactcaat tttttcacct
      841 ttattacctt tagtcacagg atattttccg acttcattta ataattttgg tgcaagaagt
      901 gtaaatataa tcatacctaa catcatgcct ataccacaaa ttaaaaatgc catcttatat
      961 ccataagata ctatatctcc acttgctcct cttactgcaa atacattatc agaaagcaat
     1021 ccacatatta ttggagctat aaatgctcct acatttactg ccatatagaa taaactgtat
     1081 gctgcatctt ttcttgacac atcagtttta ggatatatat ccccaactat atttgagcaa
     1141 cttgatttaa aaaaaccatt tcctaaaata attattgata gacctaccca aaccattgca
     1201 atagatgtag atgaagcaaa tagtacgaaa tctcctattg ctattaaaag tgcacctata
     1261 gaaatacatt tttgttgacc taaaacttta tctgctaacc agccaccgac taatggtgta
     1321 agatatataa gacctgtaaa taagccaaac atagatgttg cttcttgagt agaaagacct
     1381 aaaccacctt gagctacact tgctgtaaaa aataatacca ataaagctgt taaaccataa
     1441 taactaaatc tttcccacaa aattgtcatg caaataagcc aaaaactctt cggatgacca
     1501 ctttttttag atacgctttc tgtacttgac ataaaaaaac gccccctttt gttttatagt
     1561 taaatttaat attaatattg ctttaaccat tttagcacat acttataata tcatcttttc
     1621 tatgtttact caatagatta tagcctgtat tttcattttt ttattattat ttttaa
//
LOCUS       DAMJVK010000226         1655 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.231, whole genome shotgun sequence.
ACCESSION   DAMJVK010000226 DAMJVK010000000
VERSION     DAMJVK010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.231"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            74..808
                     /locus_tag="PC414_003843"
     CDS             74..808
                     /locus_tag="PC414_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF2466168.1"
                     /translation="MKQNKYDDDRFFNKYSKMERSTNGLAGAGEWHVLKKMLPDFKGK
                     RVLDLGCGFGWHCQYAVENGAISAVGVDISEKMLKEARNKTQLDNINYICMPIEDINF
                     PKDSFDVVISSLAFHYIQSFEDICKNISNCLSNKGNFVFSVEHPIFTAYGSQEWYCDK
                     DGTNLHWPVDGYFEEGVRKSNFLGEEVIKYHKTLTTYLNTLMKTGFEILEIIEPQPEE
                     KLLDTIPDMKDELRRPMMLLVSAKKR"
     gene            complement(951..1442)
                     /locus_tag="PC414_003844"
     CDS             complement(951..1442)
                     /locus_tag="PC414_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HDF2466169.1"
                     /translation="MNNQQQEVYKKLDELSIPYEAVNHPPVYTIEEMEELKTLHMEFV
                     VKNLFLRDAKGKEHFLVVLGKDKKADLKDIRQQIDSKPLSFASEERLQKYLKLTKGAV
                     TPFGILNDKEAVVKVVFDKDLLKMDKIGIHPNDNTATVFLKFEDMKKLIEENGNEIYY
                     VEV"
BASE COUNT          618 a          198 c          244 g          595 t
ORIGIN      
        1 aagtgataaa aacttatatt ttgtatttaa tagagtgttg ctaagtgaat atatttaaaa
       61 tggaggaaac tttatgaaac aaaataaata tgatgatgat agatttttta ataaatacag
      121 taaaatggaa cgttctacaa atggattagc aggtgctgga gagtggcatg ttttaaaaaa
      181 aatgttacct gatttcaaag gaaaaagagt tttagattta ggatgtggat ttggatggca
      241 ttgtcaatat gcagttgaga atggagcaat ttctgctgtt ggagttgata tttcagaaaa
      301 aatgttaaaa gaagctagaa acaaaacgca acttgataat ataaattaca tatgtatgcc
      361 tattgaagat attaattttc caaaagatag ttttgatgta gttattagtt ccttggcatt
      421 tcattatatt caatcatttg aagatatatg taagaatata agtaattgtc tttcaaataa
      481 aggtaatttt gtattttctg ttgaacatcc tatatttact gcatatggaa gtcaagagtg
      541 gtactgtgat aaagatggca ctaatctaca ctggccagta gatggatatt ttgaagaagg
      601 agtacgtaag tcaaattttc taggagaaga ggttataaaa tatcataaaa cattaacaac
      661 ttatttaaat acgttaatga aaacaggatt tgaaatatta gaaattattg aacctcaacc
      721 agaagaaaaa ctgttagata caattcctga tatgaaagat gagctacgta gacctatgat
      781 gttacttgta tctgcaaaaa aaagataagt aaatataaat aatcattatt gtagaaaaaa
      841 agttgtagaa aataattaca ctaatggaga tgattatacc agtggtatta attacaccag
      901 tagtaaggag gcaaaagcat tcgttgcttt tgccattttt taagttgcaa ttatacttct
      961 acgtagtata tttcgtttcc attttcttct ataagctttt tcatatcttc aaattttaaa
     1021 aatacagtag cagtattatc atttggatgt atacctattt tatccatttt taataaatct
     1081 ttatcaaata caacttttac aacagcctct ttatcattta atataccaaa aggagtcaca
     1141 gctcctttag taagtttcaa gtatttttga agtctttctt ccgatgcaaa actaagaggt
     1201 ttactatcaa tttgttgacg aatatctttt aaatctgctt ttttgtcttt acctaataca
     1261 actaaaaaat gttcttttcc tttagcatca cgaagaaata ggttttttac aacaaattcc
     1321 atatgtaatg tttttaattc ttccatttct tcaattgtat aaactggtgg atgatttaca
     1381 gcctcataag gtatgcttaa ctcatctaat tttttatata cttcctgttg ttgattgttc
     1441 atgatttcct cccattagaa atttatttta tatataaata atattatcac agaaaacttt
     1501 aaaagaaaac gttttatata atatagacaa tagattatat ttttataaag aagcaagaat
     1561 actcaataaa acttaaagaa attaaacaat ttctaaaaat accttgaaaa tagaacctta
     1621 tttgccattt ataaattata taaaaaggga ttata
//
LOCUS       DAMJVK010000227         1620 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.232, whole genome shotgun sequence.
ACCESSION   DAMJVK010000227 DAMJVK010000000
VERSION     DAMJVK010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.232"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            113..1549
                     /locus_tag="PC414_003845"
     CDS             113..1549
                     /locus_tag="PC414_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp28"
                     /protein_id="HDF2466170.1"
                     /translation="MKFYKRILTLTLSISFVFANIQLVDAISSVEKIQGKNKYEIAGK
                     IADKNAYKTAILINTSNSIADGLSASGLAGALNAPILLTEKNTIPTETSARLKNVSKV
                     YIIGGTYSISTSVENSLKSKKMKVVRIKGNDRIKTSYNVAKEINSIKKVNTVMLTNAY
                     KGEADAISIASVAARDKAPIILTNGQSIPFSTSGLKSYVIGGTASMSTTLVNNTKSTR
                     LGGSTRFETNKAIINKFYKDAREFYIAGAYELTNALVGSSLSKHGPMVLVNDGSNKSI
                     LKNAKKITSIGYIDSNIVQQCLNITNGIGDINTGVVKNVKPTTKTIKDGMYKVGKDIS
                     AGEYLITSNSGSYASYYEVTSDSTGNADSILSNDIFSGTRYITLKNGQYIKIEDSTMT
                     LAKYAKAQKAKNGKFGNGMYKIGLEIPAGEYIIMSNSSDAYYEVRNDSLGNAEGIVTN
                     DTFSGRRYITVEEGQYLILNDCYLIENE"
BASE COUNT          661 a          166 c          276 g          517 t
ORIGIN      
        1 tgtaatatgc ttaatataat ttgcaatttt acataagttt gttaagtaca taactaattt
       61 aagaatatgt atttatatat taaaaattta ttatgagggg gacaatttta ttatgaaatt
      121 ctataaaaga atattaacat tgactttaag tatttctttt gtatttgcaa atattcaatt
      181 agtagatgca atatcatctg tagaaaaaat acaaggaaaa aataagtatg aaatcgcagg
      241 taaaatagca gataaaaatg catataaaac tgctatatta attaatacta gtaactctat
      301 tgcagatgga ttaagtgcaa gtggactagc aggagcttta aatgccccta tactattaac
      361 tgaaaaaaat actataccaa ctgagacatc tgcaagatta aaaaatgtat caaaagttta
      421 tataattgga ggaacatatt ctattagtac atctgtcgaa aattcattaa aatctaagaa
      481 aatgaaagtt gtaagaatta agggaaatga cagaataaaa acaagttata atgttgcaaa
      541 agaaataaat tcaataaaaa aagtcaatac tgttatgcta accaatgcat ataaaggaga
      601 agcagatgct ataagtattg catctgtagc agcaagagat aaagcaccaa taattcttac
      661 taatggacaa agtatacctt tttcaactag tggtttaaag tcttatgtaa taggaggaac
      721 agcttcaatg agtaccactt tagtaaacaa tactaaatct actagactag gggggagtac
      781 tcgttttgaa actaataaag caataataaa taagttttat aaggatgcta gagagtttta
      841 tatagctgga gcttatgaat taacaaatgc acttgtagga tcttctttat caaaacatgg
      901 tccaatggtt ttagttaatg atggtagcaa taaaagcatt ttaaaaaatg ctaagaagat
      961 aacttctata ggatatatag attcaaatat agtacaacag tgtttaaata taacaaatgg
     1021 aattggagat attaatactg gggttgtaaa aaatgttaaa cctactacaa agactataaa
     1081 agatggaatg tataaggttg gaaaagatat ctcagcaggt gaatacttaa taacttcaaa
     1141 ctcaggaagt tatgcttctt attatgaagt tacaagtgat agtacaggta atgctgatag
     1201 tatactttca aatgatattt ttagtgggac tagatatatc accttaaaga atggtcaata
     1261 tattaaaatt gaggattcaa caatgacatt agcaaagtat gctaaagccc agaaggccaa
     1321 gaatggtaag tttggaaatg gtatgtataa aattggatta gaaattcctg ctggagaata
     1381 tattataatg tcaaattctt ctgatgcata ttatgaagtt agaaatgata gcttaggtaa
     1441 tgcagaggga atagttacta atgatacttt ttcaggaaga agatatataa cggttgagga
     1501 aggtcaatat ttaatattaa atgattgtta tttaattgaa aatgaataga tgtaattgta
     1561 tttaatagta aatcatatat tttatttaag gaaaggtgtt ttaagtttta atttaaaaca
//
LOCUS       DAMJVK010000228         1617 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.233, whole genome shotgun sequence.
ACCESSION   DAMJVK010000228 DAMJVK010000000
VERSION     DAMJVK010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.233"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(114..1292)
                     /locus_tag="PC414_003846"
     CDS             complement(114..1292)
                     /locus_tag="PC414_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2466171.1"
                     /translation="MKYHIFLIITTILIIELCNIYICKIASFGNKNITIHKNYKYIFL
                     IKPLTFLIIIFTKLPLPLYYILFYIVSHKLLLSRKSSTNNHFIPNMAVISFCILHLIT
                     VSTISLVKNTSPDIIFANMTYKLISILIASILDFILWYSMNKIKEHIYIITQNQIEFS
                     LFLKFIWFTLGYILFDSIQITFAPPSSLTSYFILGSNILLILQLIIFINHIYSIEKNI
                     YLEKEHNRLKMIKQEQLLKVNLLKKKANIDELTSAYNRKFAFEKIQHYIDNNLLFSLI
                     YIDLDKLKTINDTYGHLAGDNYIINFSSIVIENLGIDDIFARIGGDEFIVIMPKSNEN
                     TTKEFIEKISKIMDNTISFSYGIVEVSSENKMTVEEIINIADIRMYTNKQNNHKRNNN
                     "
BASE COUNT          578 a          160 c          203 g          676 t
ORIGIN      
        1 tatttttaga gttattaatc attatgtcca gatatttaaa aaaattataa tgatagagat
       61 aataagataa aaaacatagt gagactacaa gtaagctcat gtaaatattc attttagtta
      121 ttgttccttt tatgattatt ttgtttatta gtgtacattc taatatcagc aatattaata
      181 atttcttcta cagtcatttt gttttcgcta gaaacttcta caataccata gctaaaagat
      241 atcgtattat ccataatttt acttattttc tctataaatt cttttgtggt gttttcatta
      301 gatttaggca taataactat aaactcatct cctcctattc tagcaaatat gtcatctatt
      361 cctaagtttt caataactat acttgagaag ttaataatat agttatcccc agctaaatgt
      421 ccatatgtat catttatcgt ctttaattta tccaaatcga tatatatcaa tgaaaataat
      481 aaattgttat caatgtaatg ctgtattttt tcaaaagcaa attttcgatt atatgctgat
      541 gttaattcat caatgttagc ttttttcttt aataaattta cttttagtaa ttgttcttgt
      601 tttatcattt ttagtctatt atgttctttt tctaaataaa tatttttttc aatggaatat
      661 atatgattga tgaatataat tagttgtaag attaataata tgttacttcc caatataaag
      721 taggaagtta gagaacttgg tggagcaaat gtaatttgta tactatcaaa taatatatat
      781 cctaaagtga accagataaa ttttaaaaat agactgaact ctatttgatt ttgagttatt
      841 atataaatat gctcttttat tttattcata gagtaccata agataaaatc taaaatagaa
      901 gctattaaaa tactaattaa cttataggtc atatttgcaa atattatatc tggacttgta
      961 tttttaacaa gtgatattgt acttacagta ataaggtgta aaatacaaaa acttattact
     1021 gccatattgg gaataaaatg gttgttagta gaactttttc tgcttaagag caatttatga
     1081 cttactatgt aaaacaatat ataatatagt ggtaatggta attttgtgaa tattattatc
     1141 aagaaggtta atggttttat taaaaaaata tatttgtaat tcttatgtat agttatattt
     1201 ttgtttccaa aagatgctat tttgcatatg taaatgttac atagttcgat tattaatata
     1261 gtagttatga ttaaaaatat atggtatttc atagtacatc ctcttttaaa gtgaaattgt
     1321 atttacttaa aatagattag attttatata ttctatttta cagtttttat ttaggtacat
     1381 gcaaagagct tattatggat accttctttt ttattgcatg tgtctgtgtt atataaaatt
     1441 taaaaagtat tatatcttaa tatacagtat gtatgataac tatgtgtcca aatatagtaa
     1501 ttatgtatgg aaagtataat agttatgtgt ataagtatag taattacata tagcatgtat
     1561 gatagttatg tgtataagta tagtaattac atatagcatg tatgataatt atgtgta
//
LOCUS       DAMJVK010000229         1408 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.234, whole genome shotgun sequence.
ACCESSION   DAMJVK010000229 DAMJVK010000000
VERSION     DAMJVK010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.234"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            <1..975
                     /locus_tag="PC414_003847"
     CDS             <1..975
                     /locus_tag="PC414_003847"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2466172.1"
                     /translation="HAFLDEIKYKNEYSKKISVEKDNVYMLKNNGKVTHRKVNGESSS
                     IPGWENIISIASYDNSIIGIKKDGTVVSKGLGNSDSDNISKWRDIIDIECDSGTFIGL
                     KKDGTVVATGNNDYGQCNVNNWFDVVSIEITKSTIEYMKNTTVIGIKKDGKVLLTGYD
                     SLNHRKETDKWRDISKIKCGSYGIFGIKNNGTVVVSGSAYDIKNWKDIVDIHIGLCGI
                     VGIKRDGTIISTDEKFNEIIKNWKDIISAEIIDNHEGISIVGINSNGDILTSSRGQNL
                     DNIKRLEDVEYINFDDSIDNFLVGIRKDNVIFIRGINNTINEEYNEEI"
     gene            1009..1365
                     /locus_tag="PC414_003848"
     CDS             1009..1365
                     /locus_tag="PC414_003848"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2466173.1"
                     /translation="MKNYIARCLSNMGVLLAGIQVLAGIIIMCQKVEVPYDYLSDTYE
                     QYSPIMIGIGAGVIVSGIVICLVLKGLAELVENSADQTQYQKEILMLLKNNNVNEKIN
                     EVKIEQQFTNEELPKL"
BASE COUNT          612 a           99 c          267 g          430 t
ORIGIN      
        1 catgcttttt tagatgaaat aaagtataaa aatgaatata gtaaaaaaat atctgtagaa
       61 aaagataatg tatatatgtt aaaaaataat ggaaaagtaa cacatagaaa agtgaatggt
      121 gaaagtagtt ctatacctgg ttgggaaaat ataatatcta tagcatcata tgataactca
      181 ataataggaa taaaaaaaga tggtacagtt gtttctaaag gacttggaaa ttcagattca
      241 gacaatatta gtaaatggag agatataata gatatagaat gtgatagtgg aacttttata
      301 ggattaaaaa aggatggtac tgtcgttgct actggaaata atgattatgg tcaatgcaat
      361 gtgaataatt ggtttgatgt agtaagtata gaaataacaa aatcaaccat tgagtatatg
      421 aaaaatacaa cagtaatagg cataaaaaaa gatggaaaag tattacttac aggatatgat
      481 agtctgaatc atagaaaaga aacagacaaa tggagagata tatcaaagat aaaatgtggg
      541 tcttatggca tatttggaat aaagaataat ggcacagtag ttgtatcagg tagtgcttat
      601 gatattaaga attggaaaga tatagtggat atacatattg gattatgtgg aatagtgggt
      661 attaaaagag atgggacaat aataagcaca gatgaaaagt ttaatgaaat cattaaaaat
      721 tggaaggata ttatttctgc tgaaataata gataatcatg aaggaatttc tattgtagga
      781 attaattcaa atggagatat tttaacatct agtagaggtc agaatttgga taatataaaa
      841 agattagaag atgtagaata tataaatttt gatgactcga ttgataattt tttagtaggt
      901 ataagaaaag ataatgtaat ttttataaga ggaataaaca atactataaa tgaagaatac
      961 aacgaagaaa tataaaaata aatattttaa tttggaggaa tagaaaatat gaaaaattac
     1021 atagcaagat gtttatcaaa tatgggagta ttgttggcag gtatacaagt attagcgggt
     1081 ataataatca tgtgtcaaaa agtagaagtt ccatatgatt atttgtcaga tacttatgaa
     1141 caatatagtc ctattatgat tggtattggt gctggggtta tagtaagtgg aatcgttata
     1201 tgtttagttt taaagggatt agcagagtta gttgaaaatt cagctgacca aacacaatat
     1261 caaaaagaaa tattaatgtt gttgaaaaat aataatgtaa atgaaaaaat taatgaggtt
     1321 aaaattgaac aacaatttac aaatgaggaa ttaccaaaat tataaaatgt tttaaattat
     1381 atactaatac tcagtactaa ttaaagta
//
LOCUS       DAMJVK010000230         1337 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.235, whole genome shotgun sequence.
ACCESSION   DAMJVK010000230 DAMJVK010000000
VERSION     DAMJVK010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.235"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            27..101
                     /locus_tag="PC414_003849"
     tRNA            27..101
                     /locus_tag="PC414_003849"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:59..61,aa:Thr,seq:tgt)
     gene            116..200
                     /locus_tag="PC414_003850"
     tRNA            116..200
                     /locus_tag="PC414_003850"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:150..152,aa:Tyr,seq:gta)
     gene            210..293
                     /locus_tag="PC414_003851"
     tRNA            210..293
                     /locus_tag="PC414_003851"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:244..246,aa:Leu,seq:tag)
     gene            323..399
                     /locus_tag="PC414_003852"
     tRNA            323..399
                     /locus_tag="PC414_003852"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:357..359,aa:Arg,seq:tct)
     gene            407..482
                     /locus_tag="PC414_003853"
     tRNA            407..482
                     /locus_tag="PC414_003853"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:440..442,aa:Gln,seq:ttg)
     gene            571..659
                     /locus_tag="PC414_003854"
     tRNA            571..659
                     /locus_tag="PC414_003854"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:605..607,aa:Ser,seq:tga)
     gene            663..738
                     /locus_tag="PC414_003855"
     tRNA            663..738
                     /locus_tag="PC414_003855"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:696..698,aa:Phe,seq:gaa)
     gene            745..818
                     /locus_tag="PC414_003856"
     tRNA            745..818
                     /locus_tag="PC414_003856"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:779..781,aa:Met,seq:cat)
     gene            833..909
                     /locus_tag="PC414_003857"
     tRNA            833..909
                     /locus_tag="PC414_003857"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:867..869,aa:Ile,seq:cat)
     gene            933..1009
                     /locus_tag="PC414_003858"
     tRNA            933..1009
                     /locus_tag="PC414_003858"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:967..969,aa:Pro,seq:tgg)
     gene            1017..1093
                     /locus_tag="PC414_003859"
     tRNA            1017..1093
                     /locus_tag="PC414_003859"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1051..1053,aa:His,seq:gtg)
     gene            1102..1177
                     /locus_tag="PC414_003860"
     tRNA            1102..1177
                     /locus_tag="PC414_003860"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1135..1137,aa:Lys,seq:ttt)
     gene            1185..1258
                     /locus_tag="PC414_003861"
     tRNA            1185..1258
                     /locus_tag="PC414_003861"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1217..1219,aa:Cys,seq:gca)
     gene            1265..1336
                     /locus_tag="PC414_003862"
     tRNA            1265..1336
                     /locus_tag="PC414_003862"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1297..1299,aa:Asn,seq:gtt)
BASE COUNT          322 a          309 c          354 g          352 t
ORIGIN      
        1 ccccttccag gtcgccaaaa taaaatgctg gtgtggctca acggtagagc agctgacttg
       61 taatcagcag gttgtaggtt cgattcctat caccagctcc atataaaatt aatacggagg
      121 atttcccgag cggccaaagg gggcagactg taaatctgtt gtcatcgact tcggtggttc
      181 gaatccacca tcctccacca aaaaaatgcg cggatgtggc ggaattggca gacgcactag
      241 acttaggatc tagcgccatt ggcgtggggg ttcgactccc ttcatccgca ccatttttgt
      301 ttgatataca tatataaatt gtgggtgcat agctcagctg gatagagcaa cgcccttcta
      361 aggcgtgtgt ccggggttcg aatccctgtg cgctcaccac atatattggg gattagccaa
      421 gtcggtaagg cacatgactt tgactcatgt atgcgtaggt tcgagtcctg catccccagc
      481 caaatgatgt attactaagc tatatttgaa ttatttttat tttaaatata gttaggttca
      541 attttagaat aagtttaagt atccgaatgt ggagaggtgt ccgagtggtt taaggagctg
      601 gtcttgaaaa ccagtgactt cgaaaggggc cgtgggttcg aatcccaccc tctccgccaa
      661 atgcccagat agctcagtcg gtagagcagg ggactgaaaa tccccgtgtc ggtggttcga
      721 ttccgcctct gggcaccaaa atatggcggt atagcttagt tggctagagc gttcggttca
      781 tacccgaaag gtcataggtt cgactcctat taccgctact aaaatttaat gtgggccatt
      841 agctcagttg gttagagcgc ccggctcata accggtaggt ctggggttcg agtccctgat
      901 ggcccaccac attaaattaa ttaatataaa ttcgaggtgt agcgcagttt ggtagcgcac
      961 atggtttggg accatggggc cgggggttcg agtcccttca cctcgaccaa tataaggtgg
     1021 gtatagctca gttggttaga gcgccagatt gtggctctgg aggtcgtgag ttcgactctc
     1081 attatccacc ccaattcata tggtctatta gctcagtcgg tagagcacct gacttttaat
     1141 cagggtgtcc cgcgttcgag tcgcggatag atcaccaaaa aggtggcgac atagccaagt
     1201 ggtaaggcag tggactgcaa ctccttgatc cccagttcga atctgggtgt cgcctccaaa
     1261 atattccaag gtagctcaac ggtggagcaa ccggctgtta accggtaggc tgtgggttcg
     1321 agccccaccc ttggagc
//
LOCUS       DAMJVK010000231         1332 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.236, whole genome shotgun sequence.
ACCESSION   DAMJVK010000231 DAMJVK010000000
VERSION     DAMJVK010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.236"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            451..1131
                     /locus_tag="PC414_003863"
     CDS             451..1131
                     /locus_tag="PC414_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2466174.1"
                     /translation="MQIDVLIIDGDKNSSQELKNFLEGKGINVYLAYNCKDAIIKIFS
                     KKFDLIFLEIILVDGDGWDFCKKIRNITTCPIIHITYINENQSILNALNSGGDDYLIK
                     PLNLEILYAKVKAILRRRDLFLNNNKNNIKLEDYNRASGVIKLENKMIKLTPTENRLL
                     NYFIENAEKTLTIKEIYEHVWMNEYLDDNYSVVVAVSGLRKKIEKDYKNPQKIITIRE
                     VGYYFNKI"
BASE COUNT          588 a           83 c          196 g          465 t
ORIGIN      
        1 gaaattttat actttggtta aaataatttt atatatagtg cctaatgtat aacctaatgc
       61 tcgtagacaa taatgtggat attatcaaat ttatttttat aatagaaatt ttaatttata
      121 tctatattaa attgttaaag atataattat gagaataaaa aatatagctt tgaattatat
      181 aattgtatat gcttatcttt atacagtaaa taaaaaatca aatgaaatac caaataaagt
      241 gtatatctta aaataactta ctagagatac aaggatgcta tgtgaatttt gaagtgtgca
      301 gtttagataa taaatatata taaataataa agaaaatgga tttatagtat ttaaaatttc
      361 ttataagggt aaaaaatgct attagaaaat gtactaaaaa tatttttcca attctttata
      421 gaaaaataat gatatttagg agagattgta atgcaaattg atgtattgat aattgatgga
      481 gataagaata gttctcaaga gctgaaaaat tttttagaag gaaaggggat aaatgtctat
      541 ttagcatata attgtaaaga tgctattata aaaatattta gcaagaaatt tgacttaata
      601 tttttagaga taattttagt tgatggtgat ggttgggatt tttgtaaaaa aattagaaat
      661 ataactactt gcccaattat tcatataact tatataaatg aaaatcaaag tattttaaat
      721 gctttaaatt ctggaggaga tgattattta ataaagcctt taaatttaga aatattatat
      781 gcgaaagtta aagctatttt aagaagaaga gatttatttt taaataataa taaaaataat
      841 ataaaactag aagattataa tagagctagt ggagtaataa agttagaaaa taaaatgata
      901 aagttaactc caacagaaaa tagattatta aattacttta tagagaatgc tgaaaagact
      961 ctaactatta aggagatata tgaacatgta tggatgaatg agtatttaga tgataattat
     1021 agtgtagtgg ttgctgtgag tggattaaga aagaaaatag agaaagatta taaaaatcct
     1081 caaaaaatta taacgataag agaagtaggg tattacttta acaagatata aagctttata
     1141 aacagaatga atgataaggc atagataata tgttagtata ttataagagt ttttgttcta
     1201 acgatatatt tgtaaaatta gatttaattt acttaattaa attatagcag tagaataaat
     1261 ataaaaaatg tctgaaatat tatagacatc agtaaaatat ataaaactat aatataagtc
     1321 gtaataataa aa
//
LOCUS       DAMJVK010000232         1298 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.237, whole genome shotgun sequence.
ACCESSION   DAMJVK010000232 DAMJVK010000000
VERSION     DAMJVK010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.237"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(256..1137)
                     /locus_tag="PC414_003864"
     CDS             complement(256..1137)
                     /locus_tag="PC414_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HDF2466175.1"
                     /translation="MKITEGYMPFKGFKTYYRIVGENTEGKKPLVLLHGGPGSTHNYF
                     EVLDKIAESGRQVIMYDQIGCGNSFVEGHPELFNADTWIEELIELRKHLGLDEIHLLG
                     QSWGGMQAIWYAIEYKPKGIKSYILSSTLSSAKLWEKEQKRRISYMSEVDQKALLDAV
                     NTGDYSSKEYNDALERFMEMYCAGEVTEDSPECLRRPKKSGSEAYIVGWGQNEFSPTG
                     TLSGYEFTDRLHEIKEPCLVTSGAIDLCSPYIAKTMYDRIPNSKWELFEYSRHMPFVE
                     ENEKYIKVLTEWLNAND"
BASE COUNT          438 a          245 c          137 g          478 t
ORIGIN      
        1 ccaatatata aaaaaatttc taaaaaacaa taaaattaag ttataaacac caatatattt
       61 ctattatttc aataagtata caattagatt agaatctaac gctagtaata aaaaattact
      121 ttcccaaaat agatataagc ctcattttat cgttcaaata atgaaaatat atttgatttc
      181 atgcaaaaag agtgcctaat gaactaaaaa gttcattttg agacactcat ttttttatat
      241 aaatttatat agtttttaat cgttcgcatt caaccactca gttaagactt ttatatattt
      301 ctcattttct tcaacgaatg gcatatgtct tgaatactca aataattccc acttactatt
      361 aggtattcta tcatacatag tctttgcaat atatggtgaa cataaatcta ttgctccact
      421 agttactaga caaggctctt ttatttcatg taatctatct gtaaattcat atccagaaag
      481 agttcctgta ggagaaaatt cattttgtcc ccatcctaca atatatgctt cagaacctga
      541 ttttttaggt cttctcaggc actctggaga atcttctgtc acttcacctg cacaatacat
      601 ttccataaat ctttctaatg catcattata ttctttgcta gagtaatctc cagtatttac
      661 tgcatcaagt aatgcttttt ggtcaacctc actcatatat gatattcttc ttttttgttc
      721 tttctcccaa agttttgcag aagaaagagt agatgaaagg atgtaagact taataccttt
      781 tggtttatat tcaatagcat accaaatagc ctgcatacct ccccaagatt gacctaacag
      841 atgaatttca tcaagtccta gatgttttct tagttcaata agctcctcta tccaagtatc
      901 tgcattaaat agctcaggat gcccttctac aaatgaattt ccacaaccaa tttggtcata
      961 cattattact tgtctaccac tttcagctat cttatccaat acctcaaaat agttgtgtgt
     1021 agaacctgga cctccatgaa gtaatactaa tggcttcttc ccttccgtat tttctcctac
     1081 tatacggtaa tacgttttaa aacctttaaa aggcatatat ccttcagtaa ttttcatgtt
     1141 tatctcctcc aatttgtaat tataaacaat aatcaagttc attaacaata ctatttaaaa
     1201 cctcttatac atatttgatt atgtatttta atctttatac ctacttttat aaatagtata
     1261 acataaaagt taaaaatctc tatactaaac caattata
//
LOCUS       DAMJVK010000233         1208 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.238, whole genome shotgun sequence.
ACCESSION   DAMJVK010000233 DAMJVK010000000
VERSION     DAMJVK010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.238"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            36..293
                     /locus_tag="PC414_003865"
     CDS             36..293
                     /locus_tag="PC414_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2466176.1"
                     /translation="MIIIRSQDKTNLMQINQIKIDGSQVYAVFESKIDTVKIGDYENN
                     TRAIQVLDNIQNFIENGTKYDYITSNKVRYNVNKIFQMPAK"
     gene            complement(403..1083)
                     /locus_tag="PC414_003866"
     CDS             complement(403..1083)
                     /locus_tag="PC414_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDF2466177.1"
                     /translation="MIWTFYSFICVILPCLIYQIVVIKRKNLKVEKNVMIHLVWVYIF
                     LLYIYLALSKAGIGSIWDIGRYSGLFRIDEINLIPFDSVGILTYILNIIMFMPLGFLL
                     PLIWKNFRNIINVSLTGLGFSLAIELCQLFNLRATDIDDLMMNTLGAVLGYFIWVGVN
                     NLFNLIKKKDIFPSIYKNIIQDEIAITIDDDISKKITSLYKNEAIIYLILAVLGEFLL
                     FNWSIIPY"
BASE COUNT          499 a          147 c          132 g          430 t
ORIGIN      
        1 aaattaaaat gaattaagta aatggaggaa ttgatatgat aataattaga agtcaagata
       61 aaactaattt aatgcagata aaccaaatta aaatagatgg tagtcaagtt tatgcagtat
      121 ttgaaagtaa aattgatact gtaaaaatag gagattatga aaataataca agagctatac
      181 aagtgttgga caatatacaa aacttcattg aaaatggaac aaaatatgat tatataacat
      241 caaataaagt cagatacaat gttaataaaa tatttcagat gccagctaaa tagtgagtgt
      301 gcattttaat ttaataacta aagcccactt tcttttgtag tatgaaagag ggctttacat
      361 atttgtaaat atcgaagtac acttatttta tcaataaatt atttaataag gtataatgga
      421 ccaattaaac aataaaaatt ctcctaaaac agcaagtatt aaatatataa tagcttcatt
      481 tttgtaaaga gaggtaattt ttttgcttat gtcatcatca attgtaatag caatctcatc
      541 ttgaataata tttttatata tagatgggaa aatatctttt ttctttataa gattaaataa
      601 attatttaca cctacccata taaaatatcc taaaacagca cctaatgtgt tcatcattaa
      661 atcatcaata tctgtagctc ttaagttaaa caattgacaa agttcaattg ctaatgaaaa
      721 acctaatcca gttaatgaaa cattgataat atttctaaaa tttttccaaa ttaaaggtag
      781 taaaaatcct aaaggcataa acataattat atttaaaata tatgttaaga tgccaactga
      841 gtcaaaaggt attagattta tttcatcaat tctaaaaagc ccactatacc ttcctatatc
      901 ccatatgcta ccaattccag ctttactaag tgctagatag atatataata aaaaaatata
      961 tacccatact aaatgtatca ttacattctt ttcaactttc aaattttttc gttttataac
     1021 gactatttgg tatattaagc atggtaatat aacgcaaata aatgaataaa atgtccatat
     1081 cattttattt tgtccccttt caaatttatg tttttagttt tataagtact atctttatat
     1141 taaactgtaa gttttaaaaa tcttttaata aaatcttaaa aatttattaa tttataaaaa
     1201 tatattat
//
LOCUS       DAMJVK010000234         1108 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.239, whole genome shotgun sequence.
ACCESSION   DAMJVK010000234 DAMJVK010000000
VERSION     DAMJVK010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.239"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(272..955)
                     /locus_tag="PC414_003867"
     CDS             complement(272..955)
                     /locus_tag="PC414_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HDF2466178.1"
                     /translation="MTSKSQMSESFFLCSLLAITGGFLDIYTYVVRGKVFANAQTGNI
                     VLFGLNIAEGNVKESFYYLIPILAFMLGVFIAEKIRKYFTNNKTKIHWRQVIIIIEMI
                     TILIVSFIPRGRFNMFVNVAISFICSMQVESFRKVNGNSFATTMCTGNLRSATETLFH
                     YIHTKNIFMIKKSLQYYAIIIFFISGAILGTFFTKIFVEKSILICFFILAVVFGIMFI
                     NKDDSFNEN"
BASE COUNT          428 a          171 c           84 g          425 t
ORIGIN      
        1 tattaatcta tatattgcct actatactta agacatttta attaatttat acactgttta
       61 ctatacttga aatacttcta ttaatctata tattgcctac tatacttaag acattttaat
      121 taatttatac actgcttact atacttaaaa tacttctatt aacttataca ttgcttacta
      181 tattttaaat tctttaatta atctatacaa tatttgctat atataaagta tatcaaataa
      241 cagcttcatt tttttgtaaa tttacagaaa gttaattttc attaaatgaa tcatctttat
      301 tgataaacat aattccaaag accactgcca aaataaaaaa acatattagt attgattttt
      361 ccacgaaaat ttttgtaaaa aaagttccta aaatagctcc agatataaag aatataataa
      421 tagcataata ctgtaaactc ttttttatca taaaaatatt cttagtatgt atataatgaa
      481 ataaagtttc tgtagcactt cttaaatttc cagtacacat tgtcgttgca aaactattcc
      541 catttacttt tctaaaactc tctacttgca ttgaacaaat aaaagaaata gcaacattta
      601 caaacatatt aaatcttcct ctaggaataa atgaaacaat aagtattgta atcatttcaa
      661 ttataatgat aacctgacgc caatgaatct ttgtcttatt atttgtaaaa tactttctta
      721 tcttctcggc tataaataca cctaacataa atgccaaaat aggaatcaaa taataaaatg
      781 attcctttac attaccttct gctatattaa gcccaaataa aactatattt ccagtttgtg
      841 cattagcaaa gactttacct cttaccacat aagtataaat atccaaaaat cctcctgtta
      901 ttgccaacaa agaacataaa aagaatgatt cagacatctg actcttgctt gtcatatata
      961 aacctctcgc tttctaatga tagatacaaa atcaaaatca ttttagattc tatttttcat
     1021 tgatataaaa aaattattta tatttaaata ttgtctatat ttatacatta tttgtattta
     1081 tatagcattc atatttattt atatttaa
//
LOCUS       DAMJVK010000235         1098 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.240, whole genome shotgun sequence.
ACCESSION   DAMJVK010000235 DAMJVK010000000
VERSION     DAMJVK010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.240"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            163..303
                     /locus_tag="PC414_003868"
     CDS             163..303
                     /locus_tag="PC414_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2466179.1"
                     /translation="MEKSNTTVTKKEKIEDIGMKLEHLLSEDITFYELLLEILYKEKE
                     EN"
     gene            complement(300..671)
                     /locus_tag="PC414_003869"
     CDS             complement(300..671)
                     /locus_tag="PC414_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2466180.1"
                     /translation="MESLGDRIANLRKELDINQKELATKVGITEASLSRYENNLREPK
                     SEIIVRLAKALETSTDYLLGVNDNTKISKEDKLIIENLSVSEKTKKLLEKIYSLEKED
                     REAIEKMIDNAYLKRFLKEEN"
BASE COUNT          428 a          110 c          131 g          429 t
ORIGIN      
        1 aataaaagta ttattatata attaagataa ttatctaggg aaattttgga taatcaaaat
       61 atattttata aaatcaaaat acaattacat aaactttatt aaaaatttgt tgggaatttc
      121 tgactaacat taagaacgta agtttgttgg gggatggtat atgtggaaaa gtcaaacact
      181 actgttacaa aaaaagaaaa aattgaagat ataggaatga aattagaaca tttattgagt
      241 gaagatatta ctttttatga attattactt gagattttgt ataaagaaaa agaagagaat
      301 taattctctt ctttaaggaa tctttttaaa taggcattat caatcatttt ttctatagct
      361 tctctatctt ctttttcaag agaatatatt ttttctaaga gcttttttgt tttttcactt
      421 actgataagt tctctatgat taatttatct tctttgctta tttttgtatt atcatttacg
      481 cctaataaat aatctgttga tgtttctaat gcttttgcta atctaactat tatttcactt
      541 ttaggttctc ttaaattatt ttcataacgt gacaagctag cctcagttat accgacttta
      601 gtagcaagtt ctttttgatt tatatctaat tcttttcgta gatttgctat tctatctcct
      661 aaactttcca tatgtacatc actccttacc gaaattataa gttattttga taaaaagtaa
      721 aataaaatta ccaaaaatat aaaaaaaaaa ggttaaaata ataaaatata ttgaaaatta
      781 ccatttgtat atgtataata tgaattatac aaatggtaat ttgatgagag gtggtatgtt
      841 gaaataagaa actgaattaa ttaataaata catagaaagt tttgaaaacc tttaattgag
      901 atataaacaa tatgtacaaa attttttgca agtaaaatta ccaaatgtat aaaataaatc
      961 agtagcatag ataattttat gttttaatac attagattat ttaatttagt tataatggtc
     1021 aaaaatgcaa tctacaaata aaacaaagta ttatcaaaaa agtattcttt aagaaaattt
     1081 tttattagag atgtaatt
//
LOCUS       DAMJVK010000236         1089 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.245, whole genome shotgun sequence.
ACCESSION   DAMJVK010000236 DAMJVK010000000
VERSION     DAMJVK010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.245"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(226..717)
                     /locus_tag="PC414_003870"
     CDS             complement(226..717)
                     /locus_tag="PC414_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HDF2466181.1"
                     /translation="MGMIGCYTKISEENVFKLQQAEENLQDFVFEDANENSTINIDKA
                     WHAIHFTLTGCPFGGDDDNIFSKLVLSGNILMEIDGEFPVMLITANDVKKLSKAMNSL
                     EEQAFRKRFNINEMLENNIYPVMNDESEDDFFEYVWANLIELKKFIQEASDDGLAVIF
                     FIM"
BASE COUNT          420 a          160 c           94 g          415 t
ORIGIN      
        1 aagataaata aaaatatcta caactatata ttgcaaaaat ttaaaactaa tttatatatg
       61 ctaatataaa tatttataaa gacataagac aaatttacct acagatttaa atttaactta
      121 tatattttaa aaggctaata taatatgtat tttcttctat acaatcaaaa ggcttcatat
      181 ctaagatatg tgatgacact aatattcaca attttttagt aattatcaca ttataaaaaa
      241 tattacagct agtccatcat cagatgcttc ttgtataaac tttttaagct caattagatt
      301 tgcccataca tattcaaaaa aatcatcttc actctcatca ttcataacag gataaatatt
      361 attttctaac atctcattaa tattaaatct ttttctaaat gcttgttctt ccaaactgtt
      421 cattgctttt gatagttttt ttacatcatt tgctgtaatc aacatgacag gaaattctcc
      481 atcaatttcc ataagtatgt ttccacttag tactaatttg ctaaatatat tatcatcatc
      541 tccaccaaaa ggacaacctg tcaaagtaaa gtgaatggca tgccatgctt tatcaatatt
      601 gatagtacta ttttcgttgg catcttcaaa aacaaaatct tgtaagtttt cttctgcttg
      661 ctgtaattta aaaacattct cttcacttat tttagtataa catcctatca tccccataat
      721 tacattcctc ctaagtagcg tttttttaat tgtaacttat aattaaactt agttacaata
      781 aaatatattg agaaaagtat tatttcatct taaatttaaa tacctatatt atcaactaaa
      841 attctgtgtt tatagcgtaa gtattattat tttttcctaa aaattaaatg aatcagaagt
      901 tctataggca atattgacta taaataagaa ctattacttt taaaaaaaaa tttaagaaat
      961 tatactgtag aatcatgctc tacttattag ctttaaataa aaaataaatc atgttctcaa
     1021 attaaataat aaataacaat aaatttaaat tttaaactat aagagtttct gaagtgttaa
     1081 taagcatac
//
LOCUS       DAMJVK010000237         1049 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.246, whole genome shotgun sequence.
ACCESSION   DAMJVK010000237 DAMJVK010000000
VERSION     DAMJVK010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.246"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            184..486
                     /locus_tag="PC414_003871"
     CDS             184..486
                     /locus_tag="PC414_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2466182.1"
                     /translation="MEVNLHTGKDEFFKSHEVAKNYIGGLYKLIDVYKFSMESVSVLT
                     RIDLDKLNNFYAGEASLNYDELCLIESVIITPLVGAFETAEHNYNMIIKARKERKL"
     gene            521..979
                     /locus_tag="PC414_003872"
     CDS             521..979
                     /locus_tag="PC414_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2466183.1"
                     /translation="MKKTIALLLTLCAVMIPFTAFADGNDYEGTGNSQTVELIGQPNS
                     DSPSTKSVVVPGGTANLTAMSGGQLSWRVTSTLGNILGFEGNIDVYKAGKIVSSQYVG
                     FRLAGTTKTGVVTFKGLKKGSYIAKFKGKGVCTGGIILFSSPAEASFTIR"
BASE COUNT          403 a          111 c          195 g          340 t
ORIGIN      
        1 atattaagtg ttcttttttt ctattctatt taaataaaat agaatagaaa aaatgattga
       61 aaatattatt ctatcatgat agaatgaaaa aaacagaata ttctaccgtg atagaatatt
      121 ctgttttgaa gaagaagtaa atattatttg aaggttaaaa atataagaac aatggaggga
      181 attatggaag taaacctaca tacaggaaaa gatgaatttt ttaagagcca tgaagtagca
      241 aaaaactata taggtggatt atataaactt attgatgttt acaaattctc aatggaatca
      301 gtttccgttt taactagaat agatttagat aagttaaata atttctatgc tggagaagct
      361 agtttaaact atgatgaatt gtgcttaata gaaagtgtaa ttataactcc tttggtagga
      421 gcatttgaaa ctgctgagca taattacaat atgattatta aggcacgtaa agaaaggaaa
      481 ctgtaattga gaataatata aaaaaatagg gggaaaacaa atgaaaaaaa caattgcttt
      541 attactaacg ctttgcgcag taatgattcc atttacagct tttgctgatg gaaatgatta
      601 tgaaggtact ggaaattcac aaacagttga attaattggc caaccaaact cagattcgcc
      661 ttcgactaaa tctgttgttg ttccaggtgg tactgctaat ttaactgcaa tgagtggtgg
      721 gcagttatct tggagagtaa catctacgct tggaaatatt ttaggatttg aaggaaatat
      781 agatgtatat aaggctggca aaattgtatc atctcagtat gtaggcttta gattagctgg
      841 aactactaaa actggtgtag taacttttaa agggctaaaa aagggttcat atattgctaa
      901 atttaaagga aaaggtgtgt gtacaggagg tattatatta ttctctagtc cagctgaagc
      961 ttcttttaca attagataat tataaaaagt agaaaagagt gtttagaaat atatgaaaca
     1021 ctcttttttt atgcaaaatt ataaaaagt
//
LOCUS       DAMJVK010000238         1044 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.247, whole genome shotgun sequence.
ACCESSION   DAMJVK010000238 DAMJVK010000000
VERSION     DAMJVK010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.247"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            110..862
                     /gene="cwlD"
                     /locus_tag="PC414_003873"
     CDS             110..862
                     /gene="cwlD"
                     /locus_tag="PC414_003873"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase CwlD"
                     /protein_id="HDF2466184.1"
                     /translation="MEYYSVFISIHIQGDVVRKYIKHIIFSFAMICLVVVSIFEIKNI
                     SEDVIKYMPVTNKTIILDAGHGGIDPGALNKDKSTSEKDINLAITLKLRELIESSGGL
                     VILTREDDSSLYKEENNKTTRQKYNENLKNRKEIISNSNANMFVSIHLNAFEQSKYYG
                     AQTFYPKDKQDSKELSKCIQEELKRVVDKTNNREVKPRDDIYLLKENNIPSVLIECGF
                     LSNEKECKLLTDETYQEKIAWAIYIGIQKYLS"
BASE COUNT          464 a          108 c          159 g          313 t
ORIGIN      
        1 tagtgaatta aatagggaca gaaatataat atcattacat aataatatat taataccaat
       61 aaataaagat atatcaatat taaaaaaatt taaatgtttg tactaatata tggaatatta
      121 tagtgttttt ataagtatac atatacaggg tgatgttgtg agaaagtaca taaaacatat
      181 aatttttagt tttgcaatga tatgtctagt agtagtatcc atatttgaaa ttaaaaatat
      241 ttctgaagat gttatcaagt atatgccagt aacaaacaaa actataattt tagatgctgg
      301 tcatggaggc attgatccag gtgcattaaa taaggataag agtacatctg aaaaggatat
      361 taacctagca ataacactta agcttagaga gcttatagaa tcaagtggtg gccttgtaat
      421 attaactcga gaagatgaca gtagccttta taaagaagaa aataataaaa ctacaagaca
      481 aaaatataat gagaatttga aaaatagaaa agaaataata tctaattcta atgccaatat
      541 gtttgtctct atacatctta atgcatttga acaatcaaaa tactatggag ctcaaacttt
      601 ttatcctaaa gacaaacaag acagtaaaga attatcgaag tgtattcaag aagaacttaa
      661 aagagtagta gataagacaa acaacagaga agtcaaacca agggatgata tatatctttt
      721 gaaagaaaat aatattccat cagtactgat agaatgtggt tttttatcaa atgaaaaaga
      781 gtgtaaactc ttaactgatg aaacatatca agaaaaaata gcatgggcaa tctacatagg
      841 aatacaaaaa tatttaagtt gaaataacta taaaaatagg tctaaatatg taaatagctt
      901 gtattaaagc agttaaaatg cattaatata ggctattttt attttgacaa aaaaatattt
      961 aaaataaaag ttaaaaagtt gttgacttag aataatatag atgatattat atatgagtgc
     1021 ccaaaaggag caccaaaata agac
//
LOCUS       DAMJVK010000239         1012 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.248, whole genome shotgun sequence.
ACCESSION   DAMJVK010000239 DAMJVK010000000
VERSION     DAMJVK010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.248"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            195..701
                     /locus_tag="PC414_003874"
     CDS             195..701
                     /locus_tag="PC414_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HDF2466185.1"
                     /translation="MGFREVKIEELQFNPFTKIGKEWLLITAGDSEKFNTMTASWGGV
                     GVYWGKNVVTTYIRPQRYTKEFVDSNDTFTVAFFDETYREALNICGTISGRDINKIEK
                     AGLTPYFVDDTVAFEEANMIIVCKKLYHGNMPPENFDAKENDKKWYPEKDYHTMYISE
                     IIKVLVKE"
BASE COUNT          405 a           93 c          155 g          359 t
ORIGIN      
        1 ataatcaata tattttatct atattttaca cttataaaat atgaaaacta cttattgcga
       61 aagtaattga tttgttgtat tataatttac atgaattatg taggacaatc aaaagtcagt
      121 attataagag tacatatatt caaattttta acaattaatt tatataatta aaaaatgaag
      181 ggtaggatga agtcatgggt tttagagaag taaaaattga ggaattacag tttaatccat
      241 ttactaagat tggcaaagaa tggttactta taacagctgg tgattcagaa aagtttaaca
      301 caatgactgc tagctggggt ggtgttggag tctattgggg taaaaatgtt gttacaactt
      361 acataagacc acagagatat acaaaagaat ttgtagatag taatgataca tttacagttg
      421 ccttctttga tgaaacgtat agagaggctt taaatatttg tggtactatt tctggtagag
      481 atataaataa aatagaaaaa gcaggactta caccatattt tgtggatgat acagtagctt
      541 ttgaagaagc taatatgatt atagtatgta aaaagctata tcatggtaat atgccaccag
      601 aaaactttga tgctaaagaa aatgataaaa aatggtatcc agagaaagac taccatacta
      661 tgtacatttc agaaattata aaagttcttg taaaagaata attataataa aaaatatatg
      721 aaaagaactt attataagta aatgactgtg ctttcttatc tgatatctaa gtagatgcta
      781 gtaactaaat aaatattaat tttaaataaa tactattata aaaaagatga actatattta
      841 ctatagttca tctttttatt tatataagca cttatataag tagagattta tttgaataaa
      901 agtatttatt tgaaatattt gagtatactt tataagtatt tttatgctaa ctagaaacac
      961 ttaatttatg gcatttgtat ttgatttatt ttaatgtaaa taatttcaaa gt
//
LOCUS       DAMJVK010000240          983 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.249, whole genome shotgun sequence.
ACCESSION   DAMJVK010000240 DAMJVK010000000
VERSION     DAMJVK010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.249"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            27..112
                     /locus_tag="PC414_003875"
     tRNA            27..112
                     /locus_tag="PC414_003875"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:61..63,aa:Leu,seq:taa)
     gene            128..203
                     /locus_tag="PC414_003876"
     tRNA            128..203
                     /locus_tag="PC414_003876"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:161..163,aa:Met,seq:cat)
     gene            211..285
                     /locus_tag="PC414_003877"
     tRNA            211..285
                     /locus_tag="PC414_003877"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:244..246,aa:Glu,seq:ttc)
     gene            300..373
                     /locus_tag="PC414_003878"
     tRNA            300..373
                     /locus_tag="PC414_003878"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:332..334,aa:Gly,seq:tcc)
     gene            379..454
                     /locus_tag="PC414_003879"
     tRNA            379..454
                     /locus_tag="PC414_003879"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:412..414,aa:Val,seq:tac)
     gene            460..536
                     /locus_tag="PC414_003880"
     tRNA            460..536
                     /locus_tag="PC414_003880"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:494..496,aa:Asp,seq:gtc)
     gene            547..621
                     /locus_tag="PC414_003881"
     tRNA            547..621
                     /locus_tag="PC414_003881"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:579..581,aa:Gly,seq:gcc)
     gene            631..704
                     /locus_tag="PC414_003882"
     tRNA            631..704
                     /locus_tag="PC414_003882"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:665..667,aa:Arg,seq:tct)
BASE COUNT          274 a          216 c          228 g          265 t
ORIGIN      
        1 cgagccccac ccttggagcc aaaaaagccg aagtggcgga actggcagac gcacaggact
       61 taaaatcctg cgggacttac ctctcgtacc ggttcgattc cggtcttcgg cacttataaa
      121 acaatatcgc ggggtggagc agttggcagc tcgtcgggct cataacccga aggtcgtagg
      181 ttcaagtcct gcctccgcaa ccattaaaac ggctcgttgg tcaagaggtt aagacaccgc
      241 cctttcacgg cggtaacagg ggttcaattc ccctacgagt caccaattca attaaatatg
      301 cgggtgtagc tcaatggtag agttctggcc ttccaagcca gctgtgaggg ttcgatcccc
      361 ttcacccgct ccaaaaatgg gaccatagct cagctgggag agcacctgcc ttacaagcag
      421 ggggtcacag gttcgagccc tgttggtccc accattcgcg gcctggtagt tcagttggtt
      481 agaatgccag cctgtcacgc tggaggtcga gggttcgagc cccttccagg tcgccaaatt
      541 aaataagcgg gaatagttca gtggtagagc gcaaccttgc caaggttgaa gtcgcgagtt
      601 cgaatctcgt ttcccgctcc ataataactt gggtgcatag ctcagctgga tagagcaacg
      661 cccttctaag gcgtgtgtcc ggggttcgaa tccctgtgcg ctcattaaga aaagtttact
      721 ataaattata gtaaactttt atattgttta aaaagacaca ggaaacatgt atattacctg
      781 tgtcttttta tttataaact ttaaactatg taaaaattac agtatattct aatagagtta
      841 cagtatattc attaatattg taaacatacg gagggtaatg tataatagtc tacaagatgt
      901 ttatgaaggc atcagttaaa tataataaat ataactaatt agtaagagtt atatacatta
      961 tattgtatac tttatggtat tta
//
LOCUS       DAMJVK010000241          929 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.250, whole genome shotgun sequence.
ACCESSION   DAMJVK010000241 DAMJVK010000000
VERSION     DAMJVK010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..929
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.250"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            284..751
                     /locus_tag="PC414_003883"
     CDS             284..751
                     /locus_tag="PC414_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HDF2466186.1"
                     /translation="MKFLNVLVVVEDIEKSKKFYYDVLGLKVICDFGENVVLEGNISL
                     QEKKLWLEFINKSDSEVKFNGNDAELYFEEDNFDTFVERLSTMKDIDYVHLAIEHRWG
                     QRAIRFYDLDGHIIEVGETMSSVCRRFLDSGLSIDEVAKRMDVTVEYIESVLE"
BASE COUNT          353 a           64 c          158 g          354 t
ORIGIN      
        1 tattcccttg aaattaaaaa aacatagata tttcagtaat ctaaaatagt ctacaaaaat
       61 aacagtttta gcaaaattaa taagaataca tatagattta aacctaaaag tatgaacatg
      121 tctgtttttt acaagattaa aatttatatt atatgataaa attaacttaa ctatttcaat
      181 aagacttata atttttttct ttatactaga ccttattgaa acctttatag tttaattcta
      241 attatatatt ataaataaag tatgtgtata ttaaggaggt aatatgaagt ttttaaatgt
      301 tttagtagta gttgaagata ttgagaaatc aaaaaagttt tattatgatg ttttggggtt
      361 aaaggtaatc tgtgattttg gagaaaatgt tgtattagaa ggtaatatat cattacaaga
      421 aaaaaagctt tggttagaat ttattaataa gagtgacagt gaagttaagt tcaatggaaa
      481 tgatgctgag ttatattttg aggaagataa ttttgatact tttgttgaaa gattgagtac
      541 aatgaaagat atagattatg ttcatctagc tatagagcat aggtggggtc aaagagcaat
      601 tagattttat gatttagatg ggcatattat tgaggttgga gaaactatgt catctgtgtg
      661 tagacgtttt ttagatagtg gtctttcaat agatgaggta gctaaaagaa tggatgtaac
      721 tgtcgaatat attgaatcag ttttagaata gatggattat attaaagtaa aattgaggtg
      781 aaggctgtaa gtaaacttgt gtgaaaacaa atttgcttac agtttttatt tttctaaaat
      841 aaagaatata atacttgttt agctttattt gagtttttaa aaatattatg atatttctta
      901 tatatttata ctaaagttta aatttagaa
//
LOCUS       DAMJVK010000242          820 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.255, whole genome shotgun sequence.
ACCESSION   DAMJVK010000242 DAMJVK010000000
VERSION     DAMJVK010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..820
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.255"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(172..>820)
                     /locus_tag="PC414_003884"
     CDS             complement(172..>820)
                     /locus_tag="PC414_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2466187.1"
                     /translation="LNRFNLGLQNGEQQNINEIKFSIGSKLYLEIVNNKSKNGYIEGH
                     DSYSNFIIYDIEDNTFNISNKFMDNLNKEKNANPKEIGLLSKTKLDYMVKNNKLNLIV
                     TDMSNSITNLVYDINSNEIKLEDYEILDLKVDYNEASSGVRKVKLLDDKIYSISGIKN
                     SISGELKGFKGIIDVVGTKPVKFQVFDMKLKKNIYEAELINGDISLQEKLFFVAN"
BASE COUNT          283 a          127 c           62 g          348 t
ORIGIN      
        1 taaagataat ctttttttca ttatcaaaat aaatctaaac aatgaaaata tatttgattt
       61 catataaaaa aagtgtctca tgagctaaaa agtttatttt gagacactcc cttacttata
      121 cttatctgtg atacatataa aagtttaaag ttctaaaatt aattctaaca cttaatttgc
      181 aacaaaaaat aacttttctt gtaaacttat atctccattt attagctcag cttcataaat
      241 attcttttta agtttcatat caaatacttg aaattttact ggcttagttc cgactacatc
      301 aataatccct ttaaatcctt ttaattctcc tgatatactg ttcttaatac ctgaaataga
      361 atatatctta tcatccagta gcttaacttt cctaactcca gatgatgctt cattataatc
      421 tacttttaaa tcaagtatct cataatcttc taatttaatc tcattagaat taatatcata
      481 aacaagatta gttatggaat tactcatatc cgtaactata agattcaatt tattgttttt
      541 taccatataa tctagcttag ttttacttaa taagccaatc tcctttggat ttgcattctt
      601 ttctttgttt agattatcca taaatttatt acttatatta aaagtgttat cttctatatc
      661 atatataata aagttagagt aactatcatg tccttcaata tatccatttt tacttttatt
      721 atttactatt tctaagtata atttacttcc aatagaaaat ttaatttcat tgatattttg
      781 ttgttctcca ttctgtaatc ctaaattaaa tctatttaat
//
LOCUS       DAMJVK010000243          804 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.256, whole genome shotgun sequence.
ACCESSION   DAMJVK010000243 DAMJVK010000000
VERSION     DAMJVK010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..804
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.256"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            114..497
                     /locus_tag="PC414_003885"
     CDS             114..497
                     /locus_tag="PC414_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2466188.1"
                     /translation="MSTDKVVGIIDEETAELAGIEYTGKIYASSGVIKHIKKKHRCQL
                     SKDIFNDIIDTIKMVLKSPEYIGSHPKKPGKSVEFIKKIDDYILVATELDTKKGYLYV
                     SSMYAIKEAKLESRVNSGRVKQYNG"
BASE COUNT          337 a           77 c          119 g          271 t
ORIGIN      
        1 atattatact atcttttctt tttggaataa actatacttt aaatatagat atttaattta
       61 aataaatttt acagtattaa tagtaaatta tcttagaaag gaatgtgctt tatatgtcta
      121 cagacaaagt tgttggaatc atagatgaag aaacggcaga acttgcagga attgaatata
      181 ctggtaaaat ttatgcttct tctggtgtta taaaacatat aaagaaaaaa catagatgtc
      241 aactgagtaa ggatattttt aatgatataa tagacactat aaaaatggta ttaaagtcac
      301 ctgaatatat aggaagtcat cctaaaaaac ctggaaaaag tgtggagttt ataaaaaaaa
      361 ttgatgatta tattttagta gctacagaat tagatacaaa aaaaggatat ttatatgtat
      421 cttcaatgta tgctataaaa gaagcaaaat tagaaagtag agtaaacagt ggaagagtaa
      481 aacaatataa tggttaacat aatattatta attttcccta aaatattgaa aaatattatt
      541 atataagtta taatattaat atacacgtag atatttatta gttagatttg aggttggaaa
      601 aggcacccaa tacgctacta tagagtagtt aatagagatg caggatacgc cgccctgcca
      661 aatctaatta aattaaaaag agaggattaa ttcctctctt attgtttttt tgagatacta
      721 atttaatcta taatagaaag attgaaggtc gtttatacaa aaaaattttc agttatatac
      781 attaaactaa ttaaataaga tatt
//
LOCUS       DAMJVK010000244          787 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.257, whole genome shotgun sequence.
ACCESSION   DAMJVK010000244 DAMJVK010000000
VERSION     DAMJVK010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.257"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(<1..>787)
                     /locus_tag="PC414_003886"
     rRNA            complement(<1..>787)
                     /locus_tag="PC414_003886"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          172 a          238 c          182 g          195 t
ORIGIN      
        1 cgtccatccc ggtcctctcg tactaaggac aggtcttctc aaatttccta cgcctgcgac
       61 ggatagggac cgaactgtct cacgacgttc tgaacccagc tcgcgtacca ctttaatggg
      121 cgaacagccc aacccttggg acctactaca gccccaggat gtgatgagcc gacatcgagg
      181 tgccaaacct ccccgtcgat gtggactctt gggggagata agcctgttat ccccagggta
      241 gcttttatcc gttgagcgat ggcccttcca cgcagaacca ccggatcact aagtccgatt
      301 ttcatccttg ctcgacctgt atgtcttgca atcaagctct cttttgcctt tacactctac
      361 gtacgatttc cgaccgtact gagagaacct ttgagcgcct ccgttacctt ttgggaggcg
      421 accgccccag tcaaactgcc cacctgacag tgtcccaaga ccagattcat ggcctatggt
      481 tagagtccca gtactacaag ggtggtatcc caaggatggc tccgccaaaa ctggcgtcct
      541 ggtttcaaag cctcccacct atcctgtaca tgtagtacca agacccaatg tcaagctaca
      601 gtaaagctcc atggggtctt tccgtcctgt cgcaggtatc cggcatcttc accggaatta
      661 caatttcacc gagtctgttg ttgagacagt gcccaaatcg ttacgccttt cgtgcgggtc
      721 ggaacttacc cgacaaggaa tttcgctacc ttaggaccgt tatagttacg gccgccgttt
      781 actgggg
//
LOCUS       DAMJVK010000245          782 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.258, whole genome shotgun sequence.
ACCESSION   DAMJVK010000245 DAMJVK010000000
VERSION     DAMJVK010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..782
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.258"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            106..>782
                     /locus_tag="PC414_003887"
     CDS             106..>782
                     /locus_tag="PC414_003887"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2466189.1"
                     /translation="MDRFEVLKYLKFNVWHSEWPLELLKGAITKDNATGNLLLQLKMC
                     NISDKEIKSAYFTIECFNDAGDLIDNNLKYVYQDINIKPHECLGDDIAIKINDNKTRA
                     INIILEQVVFSDDKVHKYEREEGLIIPKLDKIELLNIELLEELKKDYEEYDFQYEIKY
                     MPNIIEKKAWSCCCGKINKLEKDKCDNCKRSKILQIKKINKNYLESIYKKSLKKKKEI
                     EIKLEKD"
BASE COUNT          363 a           54 c          117 g          248 t
ORIGIN      
        1 cttatatatg ttatcatttt tatttacctt atttttacaa attattccat aagtatatta
       61 taattatctt gagggaaata atcatataga gggagaaatt agaaaatgga tagatttgaa
      121 gtattaaaat atctaaaatt taatgtttgg cattcagaat ggccattaga gcttctcaaa
      181 ggggctataa caaaggataa tgctactggt aatttattat tacaattaaa aatgtgtaat
      241 atatctgata aggagataaa gtctgcatat tttacaatag aatgttttaa tgatgcagga
      301 gatttaatag ataataattt aaagtatgtt tatcaagata taaacattaa accacatgaa
      361 tgtcttggtg atgatattgc tataaaaata aatgataata aaacaagagc aataaatatt
      421 atattagaac aagtagtatt ttcagatgat aaagttcata aatatgaaag agaagaaggt
      481 ttaattatac ctaaattaga taaaatagaa ttgttaaata tagaattatt agaagaactt
      541 aaaaaagatt atgaagaata tgattttcaa tatgaaatta agtacatgcc taatataata
      601 gaaaaaaaag catggagctg ttgttgtgga aaaataaata aattagaaaa agataagtgt
      661 gataattgta agagaagtaa aatactgcaa ataaaaaaga taaataaaaa ttatttagaa
      721 tctatttata aaaaaagcct aaaaaagaaa aaagaaatag aaataaagct tgaaaaagat
      781 aa
//
LOCUS       DAMJVK010000246          753 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.259, whole genome shotgun sequence.
ACCESSION   DAMJVK010000246 DAMJVK010000000
VERSION     DAMJVK010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.259"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            <1..>753
                     /locus_tag="PC414_003888"
     rRNA            <1..>753
                     /locus_tag="PC414_003888"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          207 a          156 c          227 g          163 t
ORIGIN      
        1 tttaactgag cgttcagtta cttgacgtag acccgaaacc gggcgaccta cccatgagca
       61 ggatgaagcg aaagtaaaat ttcgtggagg tccgaaccca cgagcgttga aaagctcggg
      121 gatgacttgt gggtagcggt gaaattccaa tcgagcccgg agatagctgg ttctccccga
      181 aatagcttta gggctagcct caaggtgaga gatacggagg tagagcactg aatgtcctag
      241 ggggtattgc acctaccgaa gactatcaaa ctccgaatgc cgtcatctta tacttgggag
      301 tcagactgtg ggtgataaga ttcatagtcg aaagggcaac agcccagatc gtcagctaag
      361 gtccctaaat gtaagttaag tggtaaagga tgtgggattg cacagacaac caggatgttg
      421 gcttagaagc agccactcat tcaaagagtg cgtaatagct cactggtcga gtgatcctgc
      481 gccgaagatt tccggggcta aaacttacta ccgaagctac ggcatcagta atgatgggta
      541 ggggagcttc ccatacgggt tgaagcatga ccgtaaggac atgtggacag tatgggagtg
      601 agaatgttgg catgagtagc gagatgtggg tgagaatccc acaggccgta aacccaaggt
      661 ttccagggga aggttcgtcc gccctgggtt agtcgggacc taagctgagg ccgaaaggcg
      721 taggtgatgg acaacaggtt gatattcctg tac
//
LOCUS       DAMJVK010000247          689 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.260, whole genome shotgun sequence.
ACCESSION   DAMJVK010000247 DAMJVK010000000
VERSION     DAMJVK010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.260"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            80..>689
                     /locus_tag="PC414_003889"
     CDS             80..>689
                     /locus_tag="PC414_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDF2466190.1"
                     /translation="MLDAFITIVSSIIEIWSCKKVFDYTSQSKTDLKKLNMYFLFSVF
                     LIISSLYINIGANSRILICIFVMFVFYKSNYKVNIYKCLVISFFYWLILIGINGLSML
                     LVATVSNLDNMNVLLSGSLYRYEAIFLGKIMLGILLYVYKASFKNFKISKKEIYICIP
                     MIANLLSFFVLYDYIINLVKISPERRTAFLVIVLLLILSNISI"
BASE COUNT          240 a           58 c           88 g          303 t
ORIGIN      
        1 ttttatgcta taattagtat gtatttttta tgatatattt tataaaaaat ataatttaat
       61 catttttgag gatattatta tgttagatgc atttataaca attgtttctt ctattataga
      121 aatatggtca tgtaaaaaag tttttgatta tactagtcag tcaaagaccg acttaaaaaa
      181 actaaatatg tattttctct tttctgtttt tctcattatt tcttctttat atataaatat
      241 aggagctaat agtagaatct tgatatgtat atttgtaatg ttcgttttct ataagtctaa
      301 ctataaggtt aatatatata aatgtctggt aatctcattt ttttattggt taatattaat
      361 aggtataaat ggattaagta tgttattagt agcaacagta agcaatttgg ataatatgaa
      421 tgttttactt agtggcagtt tatatagata tgaagcaata ttcttgggaa agataatgtt
      481 aggtatatta ctttatgttt ataaggcttc ttttaaaaat tttaaaataa gtaaaaaaga
      541 gatatacata tgtattccta tgatagcaaa tttattgtca ttttttgtac tttatgatta
      601 tattattaat cttgtaaaaa tctctcctga aagaagaact gcttttttag taatagtttt
      661 actattaata ttatctaaca tttcaatct
//
LOCUS       DAMJVK010000248          664 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.262, whole genome shotgun sequence.
ACCESSION   DAMJVK010000248 DAMJVK010000000
VERSION     DAMJVK010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.262"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(<1..>664)
                     /locus_tag="PC414_003890"
     rRNA            complement(<1..>664)
                     /locus_tag="PC414_003890"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          152 a          191 c          151 g          170 t
ORIGIN      
        1 gatccagccg caccttccga tacggctacc ttgttacgac ttcaccccag ttattgattc
       61 caccttcgac gacttcttcc aaaaggttag ataatcggct tcgggcgtct ccaactcccg
      121 tggtgtgacg ggcggtgtgt acaagacccg ggaacgcatt caccgcagca ttctgatctg
      181 cgattactag taactccagc ttcatgtagg cgagtttcag cctacaatcc gaactgagag
      241 tagctttaag ggattagctc caccttacgg cttggcaacc ctctgtacta cccattgtag
      301 cacgtgtgta gccctaagca taaggggcat gatgatttga cgtcatcccc accttcctcc
      361 aggttatccc tggcagtctc tctagagtgc ccaacttaat gatggcaact aaagacaagg
      421 gttgcgctcg ttgcgggact taacccaaca tctcacgaca cgagctgacg acaaccatgc
      481 accacctgtc accaatgtcc ccgaagggaa ctctccgatt aaggagatgt cattgggatg
      541 tcaagcttag gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc cgctacttgt
      601 gcgggtcccc gtcaattcct ttgagtttca ctcttgcgag cgtacttccc aggcggagta
      661 ctta
//
LOCUS       DAMJVK010000249          660 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.263, whole genome shotgun sequence.
ACCESSION   DAMJVK010000249 DAMJVK010000000
VERSION     DAMJVK010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.263"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(154..633)
                     /locus_tag="PC414_003891"
     CDS             complement(154..633)
                     /locus_tag="PC414_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HDF2466191.1"
                     /translation="MSNKLIKNLLSGILQILFFLLGLVIVAGGFKSFMYLCFSGEATL
                     QGTIYGILMFILGVSYFIIIKSLIEVLGSSEYSLFVKENVKRFRIIGYLLLLNSLIEF
                     ISTFGTTGKGMRFLDLGFGFYFTVPVFVYFITSLMSFVIADGFVKAIKIKEDNDLTI"
BASE COUNT          259 a          103 c           51 g          247 t
ORIGIN      
        1 ttattttaat taaaacataa ttatatttta gtattataat cttttaattt tagcattata
       61 gtttatgtat tataccctaa tatattttat tttaaattac tagttagtca ctcttttcgg
      121 gttatttatc tcctatatat taaagtgtca catctaaata gttaaatcat tatcctcttt
      181 tatctttatt gcctttacaa atccatctgc aattacaaaa ctcataagag aagttataaa
      241 atatacaaat actggaacag taaaataaaa tccaaatcct aaatctagaa atctcattcc
      301 ttttccagta gtaccaaaag ttgaaataaa ctcaattaga gagtttaaaa gcaaaagata
      361 tccaataatc ctaaatcttt ttacattctc ttttacaaat aggctatatt cagaactacc
      421 taatacttct atcaatgatt ttataatgat aaaatatgat acacctaaaa taaacattaa
      481 tattccataa atagtacctt gtagagttgc ttctccacta aaacagaggt acataaaact
      541 tttaaatcct cctgcaacaa ttactaatcc taaaagaaaa aataaaattt gtaatatacc
      601 acttagtaaa ttttttataa gtttattaga catcatgcca actcctttaa aatattttat
//
LOCUS       DAMJVK010000250          642 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.264, whole genome shotgun sequence.
ACCESSION   DAMJVK010000250 DAMJVK010000000
VERSION     DAMJVK010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.264"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
BASE COUNT          256 a           50 c           75 g          261 t
ORIGIN      
        1 ccattgtaat atacactaaa aataagaatg tataaggtgg taaatttata taaaaaagaa
       61 tatgagttgt aaaatattaa ataatattgt actaattgga ataattttaa ctttattata
      121 gattatttta ataccattag tactgatggt gtattttaaa attggtataa gcattacaaa
      181 tacgaatatg ccaactgtaa tatgtaatat aagtagctat atacatatgt gctgtcccat
      241 atattgtatc attgtttata ttaaaaagat tatgtaaatt ggtatcaatc aataaatcat
      301 tttcagaaga aatacctaaa aacttaaaaa atacctatat gtgtatttag ttagatttga
      361 gatttttaca tttaatttta tagtgacaat attatattat ttttatgatg tctatttata
      421 tgcaattact ataatatcag aacttatagt atcatttgtt tcattatcaa taaggttttt
      481 tactttagtt ttgtcaattt tattaaaaaa agttattgat ataaaagata agaaggataa
      541 aactatatga cggatttcaa acataagagt ggatatttat ataatagata ttttatagtt
      601 aattttatta gattttattt aatactgtaa tattaatatc ac
//
LOCUS       DAMJVK010000251          641 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.265, whole genome shotgun sequence.
ACCESSION   DAMJVK010000251 DAMJVK010000000
VERSION     DAMJVK010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.265"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            116..406
                     /gene="cas2"
                     /locus_tag="PC414_003892"
     CDS             116..406
                     /gene="cas2"
                     /locus_tag="PC414_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HDF2466192.1"
                     /translation="MKKKNNLNYNYVFVVYDVGEKRVSKVFKVCKKYLSHFQKSVFRG
                     EITPSKIMSLKRDLENIIDKDNDFVCIIKLMNSNTFEEEILGNNSNCENMIL"
BASE COUNT          269 a           39 c           95 g          238 t
ORIGIN      
        1 atataagttt ttagaagtat tttaagatga gaaaggagaa aatactgaaa aataattatt
       61 ctattataat ttgccaaggt tttgtatatt tttcagttta aatattatat tacatatgaa
      121 aaagaaaaac aatttaaatt ataattatgt atttgtagtc tatgatgtag gagaaaaaag
      181 agtaagtaaa gtttttaaag tgtgtaaaaa atatctctcg cattttcaaa aatcagtttt
      241 tagaggtgaa ataactccat ctaagattat gagcttaaaa cgtgatttag aaaatattat
      301 agataaagat aatgattttg tatgtataat aaagttgatg aattctaata catttgaaga
      361 agaaatatta gggaataata gtaattgtga aaatatgata ttataaatct aattactata
      421 tttgtaaaaa ataatagctt attaaagagt agtaaaattg ggatataatt tatgatagag
      481 actgcaagta gatttttgtg aaaaggattt acttgcagta tttttgttat gtataactgt
      541 taacattagt gtacaaaaca ttatcaatag catttttcta taatgaagaa atactattat
      601 aatgggaaaa ttttattaat actaggtaat aatatattta c
//
LOCUS       DAMJVK010000252          577 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.266, whole genome shotgun sequence.
ACCESSION   DAMJVK010000252 DAMJVK010000000
VERSION     DAMJVK010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.266"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            <1..>577
                     /locus_tag="PC414_003893"
     rRNA            <1..>577
                     /locus_tag="PC414_003893"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          154 a          121 c          190 g          112 t
ORIGIN      
        1 caggatggac ccgcgtctga ttagctagtt ggtaaggtaa cggcttacca aggcgacgat
       61 cagtagccga cctgagaggg tgatcggcca cattggaact gagacacggt ccaaactcct
      121 acgggaggca gcagtgggga atattgcaca atgggcgaaa gcctgatgca gcaacgccgc
      181 gtgagtgatg aaggccttcg ggtcgtaaaa ctctgtcctc aaggaagata atgacggtac
      241 ttgaggagga agccccggct aactacgtgc cagcagccgc ggtaatacgt agggggctag
      301 cgttatccgg atttactggg cgtaaagggt gcgtaggcgg tctttcaagt caggagtgaa
      361 aggctacggc tcaaccgtag taagctcttg aaactgggag acttgagtgc aggagaggag
      421 agtggaattc ctagtgtagc ggtgaaatgc gtagatatta ggaggaacac cagttgcgaa
      481 ggcggctctc tggactgtaa ctgacgctga ggcacgaaag cgtggggagc aaacaggatt
      541 agataccctg gtagtccacg ctgtaaacga tgagtac
//
LOCUS       DAMJVK010000253          496 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.267, whole genome shotgun sequence.
ACCESSION   DAMJVK010000253 DAMJVK010000000
VERSION     DAMJVK010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.267"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            <1..>496
                     /gene="tuf"
                     /locus_tag="PC414_003894"
     CDS             <1..>496
                     /gene="tuf"
                     /locus_tag="PC414_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDF2466193.1"
                     /translation="GTVATGRVERGVLKVQDEVELVGLTEAPRKVVVTGVEMFRKLLD
                     QAQAGDNIGALLRGVQRNEIERGQVLAKTGSVKAHTKFTAEVYVLKKEEGGRHTPFFD
                     GYRPQFYFRTTDVTGACKLPEGIEMVMPGDNVTMEVDLINSIVVEEGLRFSIREGGRT
                     VASGV"
BASE COUNT          213 a           61 c          125 g           97 t
ORIGIN      
        1 ggaacagttg caacaggaag agtggaaaga ggagtactaa aagtacaaga cgaagtagaa
       61 ttagtaggat taacagaagc accaagaaaa gtagtagtaa caggagtaga gatgttcaga
      121 aaattattag accaagcaca agcaggggat aatataggag cattattaag aggagtacaa
      181 agaaacgaga tagaaagagg acaagtacta gcaaagactg gatcagtaaa ggcacacaca
      241 aagtttacag cagaagtata tgtacttaaa aaagaagaag gtggaagaca tacaccattc
      301 tttgatggat atagaccaca attctatttc agaacaacag acgtaacagg agcttgtaag
      361 ttaccagaag gaatagagat ggtaatgcct ggagataacg taacaatgga agtagactta
      421 ataaactcaa tagttgtaga agagggatta agattctcaa taagagaagg tggaagaaca
      481 gtagcttcag gagttg
//
LOCUS       DAMJVK010000254          493 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.268, whole genome shotgun sequence.
ACCESSION   DAMJVK010000254 DAMJVK010000000
VERSION     DAMJVK010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.268"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            188..304
                     /gene="rrf"
                     /locus_tag="PC414_003895"
     rRNA            188..304
                     /gene="rrf"
                     /locus_tag="PC414_003895"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            310..398
                     /locus_tag="PC414_003896"
     tRNA            310..398
                     /locus_tag="PC414_003896"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:344..346,aa:Leu,seq:taa)
     gene            410..486
                     /locus_tag="PC414_003897"
     tRNA            410..486
                     /locus_tag="PC414_003897"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:444..446,aa:Ile,seq:cat)
BASE COUNT          156 a           91 c          109 g          137 t
ORIGIN      
        1 attcattaag atatatgtgc agtttttaga gtgctaactc tatattttta aacttgtcaa
       61 aacttatgaa tttttaatag ttttataagg caaaaaatcg aataaaaaaa gtattgacac
      121 aataagacaa agatgttatt ataatacttg tcttagaaaa aaagttaaat gaaaatttag
      181 acaataatct ggttataata gcagagagga tacacctgtt cccattccga acacagaagt
      241 taagctctct agcgctgatg gtacttggtg ggaaactgcc tgggagagta ggacatagcc
      301 aggtgatgtg ccgaagtggc ggaactggca gacgcacagg acttaaaatc ctgcgggact
      361 tacctctcgt accggttcga ttccggtctt cggcaccaca aattattgtg ggccattagc
      421 tcagttggtt agagcgcccg gctcataacc ggtaggtctg gggttcgagt ccctgatggc
      481 ccaccacaat aat
//
LOCUS       DAMJVK010000255          465 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.269, whole genome shotgun sequence.
ACCESSION   DAMJVK010000255 DAMJVK010000000
VERSION     DAMJVK010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.269"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            <1..342
                     /locus_tag="PC414_003898"
     CDS             <1..342
                     /locus_tag="PC414_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2466194.1"
                     /translation="SLMAQFLGFSDVNMGIGTLVLGIASIIIGITLFKKFTFIKDTTA
                     IIVGSFIYQFTIYFAMSLGMLSTDLKLITAIVIISFLATGNLNISLKKNKCKASTKNK
                     SEKRGVVDVTN"
     gene            329..>465
                     /locus_tag="PC414_003899"
     CDS             329..>465
                     /locus_tag="PC414_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2466195.1"
                     /translation="MLQIKNLSKSFPNPYGEPNTIFENLSIDIEDGEFVSIIGSNGTG
                     KS"
BASE COUNT          170 a           65 c           70 g          160 t
ORIGIN      
        1 agtcttatgg ctcagttctt gggattctca gatgtaaata tgggtatagg aactttagtt
       61 cttggaattg cctctatcat aattggtatt acactattta agaaatttac ttttatcaaa
      121 gatactactg caataattgt tggctctttt atataccaat tcactatata ctttgcaatg
      181 agtttaggaa tgttatcaac tgacttaaaa ctaataactg ccatagttat aatatctttc
      241 ctagctactg gtaacttaaa tatttcacta aaaaaaaaca aatgcaaagc tagtaccaaa
      301 aataaatcag aaaaaagagg tgttgtagat gttacaaatt aaaaatcttt caaagagttt
      361 ccctaatcca tatggtgaac ctaatactat ctttgaaaat ttatcaatcg atatagaaga
      421 tggtgaattt gtaagtatca taggtagtaa tggaactggt aaatc
//
LOCUS       DAMJVK010000256          456 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.270, whole genome shotgun sequence.
ACCESSION   DAMJVK010000256 DAMJVK010000000
VERSION     DAMJVK010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.270"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            12..410
                     /gene="tnpA"
                     /locus_tag="PC414_003900"
     CDS             12..410
                     /gene="tnpA"
                     /locus_tag="PC414_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HDF2466196.1"
                     /translation="MYKSNKNIVYDCKYHIVFCPKYRKPVLVGEVEKMLKEILPYKAD
                     ELGAEIIEMETDKEHVHLLISCDPQYGIHKVVKGLKGFSSRVLRENFPHLKSSMPSMW
                     TNSYFVGTVGSVSLEVVKQYIENQQVRTKK"
BASE COUNT          180 a           52 c           82 g          142 t
ORIGIN      
        1 tgttagtttt tatgtataaa tctaacaaaa atatagttta tgattgcaaa tatcatattg
       61 tattttgccc taagtatcgt aaacctgttt tagttggcga agttgaaaag atgttaaaag
      121 aaatactacc atataaagca gatgaacttg gagcagaaat aatagaaatg gaaactgata
      181 aagaacatgt gcacttatta atatcttgtg accctcaata tggaattcat aaagtagtaa
      241 aaggtttaaa aggctttagt tcgagagtat taagagagaa ctttccacat ttaaagtcta
      301 gtatgcctag catgtggact aatagctatt ttgtaggtac tgttggttct gtgagtttag
      361 aagtagttaa acaatatata gaaaatcaac aagttaggac aaagaaatga taaaaactca
      421 tagagtgaaa ttaaacctaa ctagaacaca atttga
//
LOCUS       DAMJVK010000257          374 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.271, whole genome shotgun sequence.
ACCESSION   DAMJVK010000257 DAMJVK010000000
VERSION     DAMJVK010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.271"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(<1..372)
                     /locus_tag="PC414_003901"
     rRNA            complement(<1..372)
                     /locus_tag="PC414_003901"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           87 a          102 c           63 g          122 t
ORIGIN      
        1 aggactatta ccttctgtgg tgggtctttc caaacctctt caattacgat acctcttcgt
       61 tatgatatgt ccgcaacccc aataaagaaa actttattgg tttgggctaa tccgcgttcg
      121 ctcgccgcta cttacggaat cgaatttctt tctcttcctt caggtactta gatgtttcag
      181 ttccctgagt tcccctcatt aagctatgta ttcacttaat gatacttaga cattactcta
      241 agtgagtttc ctcattcgga aatcttcgga tcaaagttta cgtgcaactc cccgaagctt
      301 atcgcagctt atcacgtcct tcatcggctc ctagtgccaa ggcatccgcc ctgcaccctt
      361 aataacttga ccag
//
LOCUS       DAMJVK010000258          364 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.272, whole genome shotgun sequence.
ACCESSION   DAMJVK010000258 DAMJVK010000000
VERSION     DAMJVK010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.272"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            168..284
                     /gene="rrf"
                     /locus_tag="PC414_003902"
     rRNA            168..284
                     /gene="rrf"
                     /locus_tag="PC414_003902"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            291..362
                     /locus_tag="PC414_003903"
     tRNA            291..362
                     /locus_tag="PC414_003903"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:323..325,aa:Asn,seq:gtt)
BASE COUNT          117 a           61 c           78 g          108 t
ORIGIN      
        1 attcattaag atatatgtgc agtttttaga gtgttaactc tatgtaaagt actaacttca
       61 attgatgaag tatatatcgt aaaaacaatt actatttagt acttcaaaac tattgacatt
      121 taaaaataaa gatgttaata taatacttgt cttataaata ggcaatatgt ggttataata
      181 gcaaagagga tacacctgtt cccattccga acacagaagt taagctcttt agcgctgatg
      241 gtacttggtg ggaaactgcc tgggagagta ggacgtagcc acgtgaatat tccaaggtag
      301 ctcaatggtg gagcaaccgg ctgttaaccg gtaggctgtg ggttcgagcc ccacccttgg
      361 agcc
//
LOCUS       DAMJVK010000259          358 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.273, whole genome shotgun sequence.
ACCESSION   DAMJVK010000259 DAMJVK010000000
VERSION     DAMJVK010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.273"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
BASE COUNT          157 a           31 c           36 g          134 t
ORIGIN      
        1 tttgaattgt cattaaatat gccaatagtt ttatatatta ttgattttat taaaaaaaga
       61 gatatacaaa ctaataagtt gtaatctctc aaatataaaa aaacaataaa ttagctcatt
      121 ggatttttta gtccataata taattataga ttattcacca aacaatctat agaaattgtg
      181 gtattaatgt ttaaataacc aactttttct aaaataacct ttataggaat atatgagttc
      241 ataagtaaag atagcgaaat aagaaaccag tttggtatgt aaaatctaga tttttaatta
      301 aattatcaag attatatatt atagtatcaa aaatagaaaa ttaaaattaa atttttta
//
LOCUS       DAMJVK010000260          314 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.274, whole genome shotgun sequence.
ACCESSION   DAMJVK010000260 DAMJVK010000000
VERSION     DAMJVK010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.274"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
BASE COUNT          145 a           25 c           32 g          112 t
ORIGIN      
        1 gtaggtttta taaaaaaaca tatttagatt ttaacatctc aaaggagttt tcaaagctaa
       61 tatttcattt ttaaataaaa aaataaaata ttagttttga tttctggttt aaattgggtt
      121 ataataaatt gtcaaattat ttattaatat aaaatagact ataaaacaag caaaaatcat
      181 actattatat aaaagacctt aaagatagaa aactacagaa agaagccaaa atgtttttga
      241 aaaaaatgaa attttattgc aaaaaaatca aaatgaattt aacttaatag ttataatcta
      301 tttttagtgt taca
//
LOCUS       DAMJVK010000261          273 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.275, whole genome shotgun sequence.
ACCESSION   DAMJVK010000261 DAMJVK010000000
VERSION     DAMJVK010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..273
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.275"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
BASE COUNT           84 a           40 c           20 g          129 t
ORIGIN      
        1 ttgactttct tttaattctt taggatggat ttaaattttc tataaaaatc atactatttt
       61 agctgtttac ttgcttattt aatagtttta tttctttgat taaatatctt tcagaatcta
      121 agccagatgt ctcaaaaaaa ctgatttata atataatcat tttttctgct attcttattt
      181 ttccaatttt agtttcatat taaaatatac ttttcataga cactacattc tttcatactc
      241 attttcacat ttaactatac aagtagtctt act
//
LOCUS       DAMJVK010000262          232 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.276, whole genome shotgun sequence.
ACCESSION   DAMJVK010000262 DAMJVK010000000
VERSION     DAMJVK010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..232
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.276"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(<1..>232)
                     /locus_tag="PC414_003904"
     CDS             complement(<1..>232)
                     /locus_tag="PC414_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase dimerization domain-containing
                     protein"
                     /protein_id="HDF2466197.1"
                     /translation="NVIQKIQQLVPPTHPVLGDGILVLTDIKSSPYPGASVVPDYCKA
                     TFDRRLLVGETREGVLAPIQALLDEMMKEDPE"
BASE COUNT           66 a           41 c           37 g           88 t
ORIGIN      
        1 aattctgggt cttctttcat catttcatct aataaagctt gtattggagc taatacgcct
       61 tctcttgttt ctccaactaa taatcttcta tcaaaagtag ctttgcaata atctggcact
      121 actgatgctc ctggatatgg agaagatttt atatctgtta aaactaatat tccatcacct
      181 aaaactggat gagttggagg tactaattgt tgtatctttt ggattacatt ag
//
LOCUS       DAMJVK010000263          230 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.denovo.277, whole genome shotgun sequence.
ACCESSION   DAMJVK010000263 DAMJVK010000000
VERSION     DAMJVK010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..230
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.denovo.277"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
BASE COUNT          103 a           25 c           15 g           87 t
ORIGIN      
        1 cttaaaatac ttttcaaaaa aaatgatttt atttgaaatt ttttaaataa aacatcagaa
       61 acataaaata cagcacatgc tataaaaaaa tataaaaatt cattattaat tcctgccaat
      121 ctaatatgta tgatagtaaa taatattata ataatcaaaa acgtatctat aaaatttttg
      181 tgttttcatt caatatatta aacttatttt aattaggtat ctatctcaag
//
LOCUS       DAMJVK010000264        26468 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.guided.050, whole genome shotgun sequence.
ACCESSION   DAMJVK010000264 DAMJVK010000000
VERSION     DAMJVK010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26468
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.guided.050"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            147..1511
                     /locus_tag="PC414_003905"
     CDS             147..1511
                     /locus_tag="PC414_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HDF2466198.1"
                     /translation="MLLLIVTCAIYIWIGFYFSKKVKTASDFLIAGRNLPLPILAATI
                     AATSFGGGCLVGGVQWGAERGLWVGMYSTIGAALACFINAFFAGKLRSLSCDITPADY
                     IETRYGHSVFLRAYQSLVTPISILAIMGSQLISFGSVSTAFGIPYDIAVIIGVIVIII
                     YTYTSGMWGVAVSAFIQLAICIVFLPIVAYISLKLVGDNPLLMLQSMVKEPFFPGNKS
                     ISDFMYTVLPLVLGSIFSYESFLRYQCADNAKNAVKSSVIAGFILLFLAFPIGIIGAI
                     GGSLFPNISSVQVLPQMISTTLPPLISVLFLAAILAAIMSTADSLMTSMSAIISRDIY
                     NKLLHPNIEFNDLKNTLKYSQIASALTAIIGAIIALKFDSLLELLFWPAPLCTGVIFA
                     PFVIGLCWKGATKKGAIYAVIVSVILALLNMIGILTVFDRILVPIIGGSITIFIVSKF
                     SKKD"
     gene            1535..2188
                     /locus_tag="PC414_003906"
     CDS             1535..2188
                     /locus_tag="PC414_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2466199.1"
                     /translation="MSKSEKGKETKASIISAARELFFEQGYHKTTTRQIAERANINLG
                     LISYYFSSKSEIGAIIYEDIRNEMQSLIYNYHEEGTMMMFFLAMNADLCFLLKNEAYR
                     NFYLDIAYEEAILSYQRSVTEMVMKRYVNDKESIEHDELILSCIGILAMKPEIIRVYT
                     SHKYDISSQAVIKHFTKQFLINLGHSTDLCEEILEELSKYYIDIVDNFTPIITRVRT"
     gene            complement(2307..3089)
                     /locus_tag="PC414_003907"
     CDS             complement(2307..3089)
                     /locus_tag="PC414_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDF2466200.1"
                     /translation="MAFIECKFKSKYTGGETDIIVILPVANGKELFSGKEIYDFEGKF
                     KTLYLFHGLEDDQSSWIRYTNVEHYAKNKNIALVIPRVETSFYTDMAYGHQYFTFVSE
                     ELPKFVRAIFPLSDKREDNFVAGMSMGGYGALKLALSKPKEFSAVASFSGSPDMIHEL
                     ENQQIETNADILFHDFGRPEDARNSENDLLYILKNLKERNEDIPKIYQFCGDRDFLYK
                     NNQMFKKYADSLGVDVEYEECVGGHEWRLWNLWIKKFIDIIG"
     gene            complement(3206..4372)
                     /locus_tag="PC414_003908"
     CDS             complement(3206..4372)
                     /locus_tag="PC414_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDF2466201.1"
                     /translation="MKINWNTKYTTIAIYTFIIAASSIIFYLVSSQIDVFSNNLDAIF
                     TTLQPFIIGFAIAYLLNFILKFYEDRIFIKSEKLKKLKQSSKRGLGLLLTYATATLIL
                     YLFMHFVLPQVIESIVGLANDIPMYVNNATKLIDKLMTDLNLDEQYFNLAVDKWNEFV
                     TYIIKFVTDLIPILGSMLKNVASSIWNIVLGLIVSVYLLIDKEKFYGLSKKITYAVFT
                     EKQAARILELTHRSNYTFGRFLGGKILDSFIIGILTFVILTLVKMPYTLLISVIIGIT
                     NIIPFFGPLFGAIPSTLIVMFVSPIKAFWLLLIILIIQQIDGNIIGPKILGDSIGISA
                     FWILFSLLVAGKLLGFIGMVIGVPMFAVIYSIIKDIVESKLDKKGLPTDTSDYM"
     gene            complement(4511..5875)
                     /locus_tag="PC414_003909"
     CDS             complement(4511..5875)
                     /locus_tag="PC414_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HDF2466202.1"
                     /translation="MDIKELVKMQRKYYNTGKTRDISFRIEQLKKLKLVVSQNEEKIL
                     LALKKDLNKSDFEGFMTEVGMFYSELNFAIKNIRKWSKIKRVKSSMVNFPSISKVVPQ
                     PYGVTLIMSPWNYPFQLALIPLVWSLAAGNCVILKPSEYSVSTSSVVKDIVEDTFSKE
                     YVAVVQGSQEESEKLLLERFDYIFFTGSTNVGKIIMKSASEHLTPITLELGGKSPCII
                     LKDANIDLTAKRLTWGKLINAGQTCVAPDYVLVHEDRKNELIEKIKYYTNKYFGDNAC
                     NNEQFPKIINQRHFNRILSLIDKDKIVYGGNYNKETLKIEPTIVDNVNWDDNIMKEEI
                     FGPIFPILTYKDLDEVIQKIIQMPNPLALYIFTKNKYLENKLLEMIPAGGCCINDTVT
                     HIATNYLPFGGIGESGMGSYHGKAGFDTFTHYKSVLKKLNLDVPIRYAPYDNKLIKVL
                     KKIM"
     gene            complement(6187..6711)
                     /locus_tag="PC414_003910"
     CDS             complement(6187..6711)
                     /locus_tag="PC414_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDF2466203.1"
                     /translation="MKNVVVIYKSKYGSTKKYAKWLSELLSCDIFETKNINIEKVMKY
                     DTIILGGGLYACNIAGISFLKKNYKELQKKNLVVFGVGAAPYDDEVIKKLREVNFKDE
                     LNKIPCFYCRGAWDETKMSFIDYTLCSFFKKSIAKKERQGLKPAEAPLLSGEAFDWCN
                     KENLTPILDYIREL"
     gene            complement(6761..7258)
                     /locus_tag="PC414_003911"
     CDS             complement(6761..7258)
                     /locus_tag="PC414_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2466204.1"
                     /translation="MDAESLMKKYMVDDDSRKQAIFASIFVMQNKLQTTCDKLDPELT
                     MKQWLLLAIADSVDETLTLTKLGELMGCSRQNVKKLAIALEKKGFIQIEQNEKDSRAI
                     CLVTKDRVQEYSKKVRGMQEKVLQLLFEDFTEKEVEQLFFGIMKLSIGIENVKNYVEN
                     NDVGE"
     gene            complement(7491..8768)
                     /gene="hflX"
                     /locus_tag="PC414_003912"
     CDS             complement(7491..8768)
                     /gene="hflX"
                     /locus_tag="PC414_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDF2466205.1"
                     /translation="MDMAKKGITVGLNINNKSEDFNELMIELENLCSACDIDVVGSIT
                     QNAKQVNRAFYIGTGKVEEILNLIKKENIEIVIFYNELSTSQLKNLEEKLNCEIIDRT
                     ALILDIFAQRAKTREAKLQVEVASLKYMLPRLIGSNENLGRQSGGVGTKNRGSGEKKL
                     ELDRRRIEEKITSLNRELDDLKFQRETQRSMRRKSNLPNVALVGYTNAGKSSIMNKLV
                     DIFKNSEEKKVFEKNMLFATLETSVRNIVLANNKEFLLSDTVGFVSNLPHDLVKAFRS
                     TLEEACEADVLLHVIDISNPSYKSHIKVTEDTLKQIGADGIPMIHVYNKIDLIDVEVL
                     DRILDSIDKEGIFVSVKKDINIDKMIKCICDSIFKDYVRCKFLIPYDKGHVVSYFNEN
                     TSIINTEYREDGAILDVECSNIEYNKYKKYALE"
     gene            complement(9487..11319)
                     /locus_tag="PC414_003913"
     CDS             complement(9487..11319)
                     /locus_tag="PC414_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2466206.1"
                     /translation="MSRMGRGPMGKSMGAGQKANDFKGTMRKLIAYLSKFKISIILVI
                     VFAIGSASFSIVGPKILGKATTKIFEGLVSKVSGGNVGIDFNAIGKILTFLLFLYLIS
                     ALFSFIQGFIMSGISQKVSYNLRKEISAKLDRLPMKYFDTKTHGEILSRITNDIDTLN
                     QSLNQSMTQLITSVTTMIGVLIMMLSISGIMTLVAVLILPISMFVISRIIKKSQKYFR
                     YQQEYLGNVNGQVEETYSGQTIVKAFNREDEVIEEFDKLNDSLYNSAWKSQFLSGIMQ
                     PLMMFIGNLGYVMVSILGGWLAIKKTIEVGDIQSFIQYVRNFTQPMTQIAQVANLLQS
                     TAAASERVFEFLEEEEEVQIVENAVSIDGLEGKIDFENVNFGYNPNKTIINDFSVNVK
                     PGQKVAIVGPTGAGKTTIVKLLMRFYDVNSGSILIDGHNIKDFNRSELREMFGMVLQD
                     TWLFSGSIMENIRYGKLNATDEEVIEAAKSAHVHRFIKTLPDGYKMKLNEEASNVSQG
                     QKQLLTIARAILADPKILILDEATSSVDTRTEVLIQKAMDNLMEGRTSFVIAHRLSTI
                     RDADMILVMNEGDIVEQGNHEELLKKGGFYANLYNSQFEEDEAM"
     gene            complement(11312..13558)
                     /locus_tag="PC414_003914"
     CDS             complement(11312..13558)
                     /locus_tag="PC414_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2466207.1"
                     /translation="MFKLIKYLKKSALSIVIIVCLLVIQAVCDLSLPEYTSNIVNVGI
                     QQGGVENSVPSVIRESELNKITLFMDKSSKDKVLDNYTLLNKKDYVKYKDKYPGLKDE
                     SLYELNTKDKDTIDDLNVIFGKAILIVSGLEGDSKEVKAMKAQLMSKLPPQATQSGDV
                     DIFKLLSAMPKEQLDTLTKEMSKGFESMPESMITQSSVSYVRSEYEKIGIDTEKTQNN
                     YILFTGAKMLGIAMISMVATITVGFLAARVAASVGRNLRSGVFRKVMSFSNTEMNEFS
                     TASLITRSTNDIQQIQMLMVMLLRIVFYAPIMAIGGVIKVLNTNTSMAWIIAVAVVAI
                     LSLIVVLFSVVMPKFKLVQKLVDKLNLVSREIITGIPVIRAFSTEKHEEERFDKANKN
                     LTKTNLFVNRVMTCMMPAMMLIMNLITVLIVWRGAYSVDSGAMQVGDMMAFIQYTMQI
                     IMSFLMISMVSIMLPRASVSATRIDEVLVSDISIKDPEKSEAQEFKEDKKGLVEFKNV
                     SFMYPDAEEPILTNISFTAKPGETTAFIGSTGSGKSTLINLIPRFFDVTEGEILLGGV
                     NIKNVSQHDLREKIGYVPQKGVLFSGTIESNLKYGRKDATDEEMRTAAEIAQATEFID
                     SKPEAFKTEISQGGTNVSGGQKQRLSIARAIAKNPEVYIFDDSFSALDLKTDAALRKA
                     LKSKTAESTVLIVAQRISTILNAEQIIVLNEGEIVGIGTHKELLKNCEVYEQIALSQL
                     SKEELANE"
     gene            complement(13626..14087)
                     /locus_tag="PC414_003915"
     CDS             complement(13626..14087)
                     /locus_tag="PC414_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2466208.1"
                     /translation="MNSNSEEKLNDVFLQLMRIYFIRTHFYFDKLGLYHGQPQILFSL
                     KMKDGQSQKELADKRKVKASTTTVMIKRMEKAGLVERKQDEKDQRVSRIFLTDKGLEI
                     CNKVEEFNKELEREFFLGFSEEEKIVLRRLLMQVRDNLKAGCHKHEDEKYF"
     gene            complement(14392..14958)
                     /locus_tag="PC414_003916"
     CDS             complement(14392..14958)
                     /locus_tag="PC414_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HDF2466209.1"
                     /translation="MRKLEEILEYNKSFVENKEYEQYVTSKHPNKKIVVLSCMDTRLT
                     ELLPKAMNLKNGDVKLIKNAGATIMHPFGSIMRSVLVAIYEFDVDEVMVVGHHGCGMC
                     NVDTDKLLGKILDRGISNDVILTLRSAGIDTKQWLHGFDSAEESVKDSIDLIKGHPLI
                     PDGIIVHGLIISPETGKLDVVVNGYEDK"
     gene            15645..15983
                     /locus_tag="PC414_003917"
     CDS             15645..15983
                     /locus_tag="PC414_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2466210.1"
                     /translation="MANIGKIIGDRIKVYRTKLGYSQEFLAEKAGLHPTYIGQIERAE
                     TNTSINIIMKIATALDIPLELLFANIITTEGVDNSVPLECYEMINKLTEKEQIAMLEL
                     IKCIIKYKEI"
     gene            16005..16322
                     /locus_tag="PC414_003918"
     CDS             16005..16322
                     /locus_tag="PC414_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2466211.1"
                     /translation="MNYIGKRLKEERKKANLTSKEFANMVGVSPWYITQIESGKKNPS
                     LKTFIKFVNILNISADVLIKDITSTGKTYLENDINEELKDLNSRELNLISQIIDIIKK
                     NKI"
     gene            16472..17149
                     /locus_tag="PC414_003919"
     CDS             16472..17149
                     /locus_tag="PC414_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2466212.1"
                     /translation="MIVIEGSDKFKIAKEYIDVEYTLFSKVTFRYEKLKFKDNAELEK
                     IKMFKYKNGYIPNKLNLSFGYGFSSYKKQIIRETVDTLRLTEIFSSENIEDIKFIKDG
                     TKKLEISIEKVVKFKRRKKRNYVCCYCPDMYRDIKLDKESINKIYNRKIKIEREVNIF
                     EDEDVIINKKVLKFPKSWTKNMQKYWLSENKYPIHSTVIDDDRYKCCNVQYTKNRVII
                     LYYIYNH"
     gene            complement(17477..18244)
                     /locus_tag="PC414_003920"
     CDS             complement(17477..18244)
                     /locus_tag="PC414_003920"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDF2466213.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKKDIIKEAKELKDACLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYIHDIPLLFTFRSVVEGGEKLISRDYYTTLN
                     KEISNTGLVDLIDVELFMGDEVIDEVVNFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELGADLPKIAVMPQNEKDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELNSVLNLLHKSIN"
     gene            complement(18916..19731)
                     /locus_tag="PC414_003921"
                     /pseudo
     CDS             complement(18916..19731)
                     /locus_tag="PC414_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897377.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            19832..20530
                     /locus_tag="PC414_003922"
     CDS             19832..20530
                     /locus_tag="PC414_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2466214.1"
                     /translation="MYKLSYVVRVSTKNLDELKRRCDEVKDFYDDLSIKLVRPFGDML
                     GLHGEFIPVSKRYINDYIQYVTSDFLAGLGFGATQTLGEHEDIYLGYNLDTGKNVYLK
                     PALASQGVKGSITNALASAFIGSLGGGKLFSNNMLVYYAVLFGGQAVIVDPKAERGKW
                     KETLPEIAHEINIVNLTSNDENKGLLDPYVILSRKKDSESLAIDILTFLTGISSRDSD
                     KFLQKNDNWKIIME"
     gene            20618..21238
                     /locus_tag="PC414_003923"
     CDS             20618..21238
                     /locus_tag="PC414_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDF2466215.1"
                     /translation="MHLIDFMNEDIKKKLVKKTYKKKETILFAEQENEYVILMIDGIA
                     EAFILNVKGNISTIHLYRSGSFFGEMEQFNDGKKPVEIVAFTDCNVYKLHRDDFLNWL
                     RGDFEATKFLIREISSKLVVNAELIEELSSLSVKERLLRCISLHYYRSELNLLTKKQL
                     ASEVNAPIRSINRAINECVQEHLIAYENKRFSVINQREVLKNLPTT"
     gene            21394..22176
                     /locus_tag="PC414_003924"
     CDS             21394..22176
                     /locus_tag="PC414_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HDF2466216.1"
                     /translation="MTKTQQKMIQNVLEFSKRDSRIIGLAISSSYITKSLDEYSDLDF
                     LIVADDKSYEEVIEERLLIVEQFGTLVSAFTGEHVGEPRLIISLYDSPVLHVDFKFTT
                     LDGLLDRVEDSAILYEENNCITEIYSKKEAYFPTPDLQWIEDRFWIWVHYACTKIGRR
                     ELFETIDFLSFLRQSVLAPLIQLLLGKLPRGVRKIELDAPQYIEALEKTVANHEYKSC
                     VNALQATINLYLELREELSQSPIVRKVEAERVALQYFQDLIK"
     gene            22301..23227
                     /locus_tag="PC414_003925"
     CDS             22301..23227
                     /locus_tag="PC414_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2466217.1"
                     /translation="MSDILSRITNKIGDKNIVDKLSALSKSDLNSLLLEVFDRQANTL
                     TATDILKSYQLNRFTIPSGIDPEEIHALESKLLRKAFNMDIKTIMLSPSAPLGSCSVF
                     GSVDQYNVVSALRGTETLADPSNMLAIIIADKLKRKEENNTIPIHMATTARVIRAQNF
                     VGKGLYSHFGLYCMVSSGIDTGSYTCEKMLLEKHLNYYKDILSEIENVRFSIILRKRN
                     GYKDNDGFFDRMVETIKLIFPNIELSIHNDDVDNNYYKGINFKLSVKQGNETVEIVDG
                     GFVDWTYQMLGNKRERCLISGIGLERFLVAVS"
     gene            23241..23690
                     /locus_tag="PC414_003926"
                     /pseudo
     CDS             23241..23690
                     /locus_tag="PC414_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729090.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            24050..24457
                     /locus_tag="PC414_003927"
     CDS             24050..24457
                     /locus_tag="PC414_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2466218.1"
                     /translation="MVDEKTIEHQFDSFCKTVLRNYARDIYDENKRRNDYLVSLESLS
                     LAELSKLSILDDYDSNYICMVSYDYNIRIEDVLMAQAIGKLTKRKQDIILLSFFFNMT
                     NADIATLMDLAENTIHYHKTNALKELKELMQEH"
     gene            24459..24689
                     /locus_tag="PC414_003928"
     CDS             24459..24689
                     /locus_tag="PC414_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2466219.1"
                     /translation="MKPTKEVLPFTIILLAVHGDVNAINVILKHFEHYIIRLSQKTLF
                     DEYGNPYIHVEEETKRMLGTKLITAILRFNLS"
     gene            25079..25282
                     /locus_tag="PC414_003929"
     CDS             25079..25282
                     /locus_tag="PC414_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HDF2466220.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKRADENLDSGWVI
                     MNSNRIQIKRKQFEKIVDTLDVI"
     gene            25360..25713
                     /locus_tag="PC414_003930"
     CDS             25360..25713
                     /locus_tag="PC414_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase DNA-binding domain-containing protein"
                     /protein_id="HDF2466221.1"
                     /translation="MSTEKRRDNKDRILRTGESQRKDGRYAYKYVYSFGKPQFVYSWK
                     LVPTDKPPKGKRDDISLRDKEKAIQNDLDDSIDTIGKKMTVCQLYAKKNGLRKNIRLN
                     TKKGRHYLMKILNKE"
     gene            25785..26417
                     /locus_tag="PC414_003931"
     CDS             25785..26417
                     /locus_tag="PC414_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HDF2466222.1"
                     /translation="MTIPSSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVPPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKLV"
BASE COUNT         9213 a         4152 c         3421 g         9682 t
ORIGIN      
        1 tacgtaattt ccatctaata tcataaatta ttatagctat attaatctaa aaccaataaa
       61 acaaatatcg tactactttt atataaaata tattttaaat atattttgta tattttatta
      121 aaatatttta taaaggagaa aagactatgt tacttttaat tgttacgtgt gcaatataca
      181 tatggattgg tttttatttt tcaaaaaaag ttaaaacagc atccgatttc ctaatcgcag
      241 gtagaaatct gcctttgcct atactagctg caaccattgc tgctacatct tttggaggag
      301 ggtgcttagt tggtggagtt caatggggag ctgaaagagg actttgggtt ggaatgtatt
      361 ctactatagg tgcagcttta gcttgcttta taaatgcatt tttcgcagga aaattaagaa
      421 gcttatcttg tgatataaca cctgctgatt atattgaaac tagatatggt catagtgtat
      481 ttttaagagc ttatcaaagt cttgtaacac caatatctat actagctatt atgggtagtc
      541 aattgatttc ttttggttct gtatctactg catttggtat tccttatgat atagctgtaa
      601 ttataggagt tatagttatc attatatata cttatacatc aggaatgtgg ggagttgcag
      661 tatcagcatt tattcaactt gctatttgta tagtgttttt gccaatagta gcttatataa
      721 gcttaaaatt agttggcgac aaccctttac ttatgttaca atcaatggta aaagaaccat
      781 tctttccagg aaataaatcg attagtgatt ttatgtatac agttttacca ttagttttag
      841 gctcaatatt ttcttatgaa agttttttaa gatatcaatg tgctgataat gctaaaaatg
      901 cagttaaaag tagtgtaatt gcaggattta tactattatt tcttgctttc cctataggaa
      961 taattggagc tattggtggt agtttatttc caaatatatc atctgttcag gttttacctc
     1021 aaatgatatc gactactttg ccacctttaa tatctgtatt attcttagct gctatattgg
     1081 ctgccataat gtctactgcc gattcattaa tgacctcaat gagtgctatt atatcaagag
     1141 atatttataa caaattatta catcctaata tagaatttaa tgatttaaaa aatactctaa
     1201 agtattctca aattgcatca gctttgacag ctataatagg tgcaatcata gcacttaaat
     1261 ttgatagttt attagaatta ttgttttggc cagctccatt atgtacaggt gtaatatttg
     1321 caccatttgt aattggtctt tgctggaaag gtgctacaaa aaaaggagct atctatgctg
     1381 ttatagtaag tgttatattg gcattattaa atatgattgg tattcttact gtatttgaca
     1441 gaattttggt tcctataata ggtggttcta ttacaatatt tatagttagc aaattttcaa
     1501 aaaaagattg aaaagagagg tatacacagt aaatatgtca aaatcagaaa agggcaaaga
     1561 aacaaaagct agtattataa gtgctgctag agaattattt tttgagcaag gttatcataa
     1621 gacaacaact aggcaaattg cagaaagagc taatataaat ttaggtctaa taagctatta
     1681 cttctcaagt aaaagcgaaa taggagctat aatatatgaa gatataagaa atgaaatgca
     1741 gtctttaatc tataactacc atgaagaagg tactatgatg atgttctttc ttgctatgaa
     1801 tgctgacctt tgtttccttc taaaaaacga agcatataga aatttttatt tggatatagc
     1861 ttatgaagaa gctattttaa gctatcaaag aagtgtaacc gaaatggtca tgaaaagata
     1921 tgttaatgat aaagaatcta tagaacatga tgaattaata ctttcttgta ttggtatact
     1981 tgctatgaaa cctgaaataa ttagagttta tacttcacac aagtacgata tatcttctca
     2041 agctgtaata aagcatttta caaaacagtt tcttattaat cttggtcata gtacggattt
     2101 atgcgaagag attttagagg aattgagtaa atactatata gatatagttg acaattttac
     2161 tccaataata actcgtgtaa gaacttaata aaattttatt aaattcttag agactgaaat
     2221 tttaaaatca taatttgatt ttaggatttc agtctttatc atatttaaag atattttata
     2281 gtatacctgt taataccata agtgtactat ccaattatat ctataaattt ctttatccat
     2341 aaattccaaa gacgccattc atgcccccct acacactctt catattcaac atctacaccc
     2401 agagaatctg catatttttt aaacatctga ttatttttat ataaaaaatc tctatcacca
     2461 caaaattgat aaattttagg aatatcttca tttctttctt taaggttctt aagtatgtaa
     2521 agtaaatcat tctcactatt tctagcgtct tctggtctac caaaatcgtg aaatagaatg
     2581 tctgcattgg tttctatttg ttgattttct aactcatgaa tcatatcagg cgaaccagaa
     2641 aaacttgcaa ctgccgaaaa ctcttttggt tttgaaagcg ctaattttaa agctccatat
     2701 ccccccatag acataccagc tacaaagtta tcttcccttt tatctgataa aggaaatata
     2761 gctctaacaa atttaggtaa ttcctcgctt acaaaagtaa aatattggtg tccatacgcc
     2821 atatcagtat aaaaactagt ttctactctt ggtattacta gggctatatt tttattttta
     2881 gcataatgct ctacattagt atatctaatc catgagcttt ggtcatcttc taaaccatga
     2941 aataagtata atgttttaaa tttaccttca aaatcatata tctctttacc actaaaaagt
     3001 tcttttccat ttgctacagg caaaattaca attatatcag tttctcctcc agtatattta
     3061 gatttaaatt tacattctat aaatgccaca aaaaacacct cttctataat aattttatat
     3121 ctaaaaagca ccaagagttt aactttctca gtgctttaaa tattgcttaa tttatatatt
     3181 tttaattata cataatattt tttagctaca tataatcgga agtatctgtc ggtaatccct
     3241 ttttgtccaa tttactttca acaatatcct taattataga ataaattact gcaaacattg
     3301 gaactcctat aaccattcca ataaaaccta ataattttcc tgcaaccaat aaggagaaca
     3361 aaatccaaaa tgcagatata ccaattgaat ctccaagtat tttaggtcct ataatatttc
     3421 catctatctg ttgtattatt aatataatca acaataacca gaaagctttt ataggagata
     3481 caaacatcac tattagcgta gatggtatag ctccaaataa aggtccaaag aaagggataa
     3541 tatttgtaat accaattata actgatatta ataatgtata tggcatttta actaacgtaa
     3601 gtattacaaa tgttagaata cctataatga atgaatctaa tattttgcca cctaaaaatc
     3661 ttccaaatgt atagttactt ctatgagtta attctaaaat tctggcagct tgtttttctg
     3721 taaatactgc ataagttatt tttttgctta atccatagaa tttttcttta tcaattaata
     3781 agtacactga aacaattaat cctagtacaa tattccatat actagaggct acattcttta
     3841 acatacttcc taatattggg attaaatctg ttacaaactt tataatatat gtaacaaatt
     3901 cattccactt atcaactgcc aaattaaagt attgctcatc aagatttaaa tcagtcatta
     3961 atttatcaat aagcttagtt gcattgttta catacatagg tatatcattt gctagtccaa
     4021 caatactctc aattacttgt ggcaatacga aatgcataaa taagtaaagt atcaatgtag
     4081 ctgttgcata tgttaataaa agacccaagc ctctttttga actttgtttt agttttttta
     4141 atttctcact ttttataaaa attctgtctt cataaaactt taaaataaaa ttaagaagat
     4201 atgcaatagc aaatcctatt ataaaaggtt gtaatgttgt aaatatagca tctaaattat
     4261 tagaaaatac atcaatttgt gatgatacta gataaaatat aatactacta gcagctatta
     4321 taaatgtata aattgctatt gttgtgtatt ttgtattcca attaattttc attttttcct
     4381 ccttatttct atataattaa attttatcac aaaatcacca cttttataac aaatatacca
     4441 aattaaaaat aaaatatatt cagaatacaa atactcaatt ttatttatat ttattattct
     4501 acaatataac ctacattatc tttttaagaa ccttaattaa tttattatcg tatggagcat
     4561 atctaatagg gacatccaaa tttaattttt ttaacacact cttatagtga gtaaatgtat
     4621 cgaatccagc ttttccatga tatgagccca taccactttc accaatacca ccaaaaggta
     4681 agtaattcgt agctatatga gtaactgtat cattgataca acatccgcct gctggaatca
     4741 tttctaaaag tttattttct aggtacttat tttttgtaaa aatataaagt gccaatggat
     4801 ttggcatttg tataattttt tgtattactt catctaaatc tttatatgtt aaaatgggaa
     4861 atataggccc aaatatttct tccttcatta tattatcatc ccaattaaca ttgtctacta
     4921 tagttggctc tatctttaat gtctccttat tataatttcc accatagact atcttatctt
     4981 tatcaataag agataatatc cgattaaaat gtctctgatt tatgatttta ggaaattgct
     5041 cattattaca tgcattatcc ccaaaatact tattagtata atattttatt ttctctatta
     5101 actcattttt tctatcttca tgcactaaaa cataatctgg agccacgcag gtttgtcctg
     5161 catttattag ttttccccaa gtaagtcttt tggcagtaag gtctatatta gcatctttta
     5221 aaatgataca aggacttttt cctccaagtt ctaaagtaat aggagttaaa tgctctgatg
     5281 ctgatttcat gattatttta ccaacattag tacttcctgt aaaaaatata taatcaaatc
     5341 tttccaatag taacttttca ctttcttctt gacttccttg aacaactgca acatattctt
     5401 ttgaaaaagt atcttcaact atgtctttaa ctacactaga tgttgacaca gaatattcag
     5461 aaggttttaa tataacacaa ttccctgctg ctaatgacca aactaatggt ataagtgcta
     5521 attgaaatgg ataattccaa ggtgacatta tgagagtaac accataaggt tgtggaacaa
     5581 ctttggatat agatggaaaa ttaaccatac ttgatttaac tctttttatt ttgctccatt
     5641 ttcttatatt ctttatagca aagtttaatt cactataaaa catgcctacc tctgtcataa
     5701 aaccttcaaa atccgattta tttaaatctt tctttagtgc taaaagtatt ttttcttcat
     5761 tttgtgatac aactaactta agtttcttca actgttctat acgaaacgat atatctcttg
     5821 ttttacctgt attataatat tttctttgca ttttaactaa ctcttttata tccattgtta
     5881 aaaatccttt ctttaagaat tatactaata catttttaga aaaagattta aatttatcct
     5941 aacattattt tttatatcat aataaattca tctcaatata acataaatct tccaataaaa
     6001 aaagcccctt attaagtata agtaagctta taaaatagtt tattttaaaa taaacatttt
     6061 ttaaaattat aaccttattg aaacaattaa attatccatc tttaataata ttattttata
     6121 aataatgtct agtaagtttc tatgaaatct aactttagat aataaataat tacaaatata
     6181 tacctcttaa agttctctta tataatcaag tattggagtt aaattttctt tattacacca
     6241 atcaaaggct tctccagaca ataaaggtgc ttctgctggt ttaagacctt gacgttcttt
     6301 tttagctata gattttttaa aaaaactaca aagagtataa tctataaaag acatttttgt
     6361 ctcatcccat gccccacgac aataaaagca aggaattttg tttaattcat ccttaaaatt
     6421 tacttctctt aatttcttta ttacttcatc atcatatgga gcagctccta cgccaaatac
     6481 tactaaattt tttttctgca attctttata attttttttt aagaaagaaa ttcccgctat
     6541 attacaagca taaagacctc ctcctaaaat aatcgtatca tacttcataa ccttttctat
     6601 attaatattc ttagtttcaa aaatatcaca agacaataat tctgaaagcc atttagcata
     6661 tttttttgtt gaaccatact tactcttata aatcactact acatttttca tataaattat
     6721 ccccctctct gatgtcatac aaagttgatt tgatacttgc ttattctcca acgtcattat
     6781 tttctacata atttttcaca ttttcaattc ctatacttaa tttcataatt ccaaaaaata
     6841 actgttccac ttctttctct gtaaaatctt caaataaaag ctgtaatact ttttcctgca
     6901 tacctcttac ttttttagaa tactcttgca ctctatcttt tgtcacaaga cagatagctc
     6961 ttgaatcttt ttcattctgc tcaatttgta taaaaccttt cttttctaaa gcaatagcta
     7021 gtttttttac attttgtctt gagcacccca ttaattctcc aagtttggtt aatgtaagag
     7081 tttcatctac agagtccgca atagctaaaa gtaaccattg tttcattgtt aattctgggt
     7141 ctaatttatc acatgtagtt tgaagtttat tttgcattac aaatatactt gcaaatatag
     7201 cttgttttct actatcatca tctaccatat attttttcat taaactttct gcatccatta
     7261 ttatacactt ctctttatct tagattttaa ttttttgata attttaaata gtaactaatt
     7321 actttatatt atagtaatta gttactatta tgtcaataaa tttattttta ttataaacgg
     7381 caaaacggca tagcattatg gctataccgt ttcataaaat catatttaca atcaatttat
     7441 aataaattat aaattaaata tattttaggc tataataagt ttttcaaaat ttactctaaa
     7501 gcatactttt tatacttatt atattctata ttgctacatt ctacatctaa aatagctcca
     7561 tcttctctgt attctgtatt tataatgctc gtattttcgt taaaataaga aactacatga
     7621 cctttatcat atggaattaa gaacttacat cttacataat ctttaaatat gctatcacaa
     7681 atacatttta tcattttatc tatatttata tcttttttaa ctgaaacaaa tataccttct
     7741 ttatcgatac tatctaaaat tctatccaat acttctacat ctattaaatc aattttatta
     7801 tacacatgta tcataggtat accatctgca cctatttgtt ttaaagtatc ttctgtcact
     7861 ttaatatgac ttttatagct tggatttgaa atatcaataa catgtaacag tacgtctgcc
     7921 tcacatgctt cttctaatgt tgacctaaat gcttttacta agtcatgtgg tagattactt
     7981 acaaaaccaa cagtatcaga taataaaaat tctttgttat ttgcaagaac tatatttctg
     8041 acagaagttt caagtgtagc aaataacatg tttttctcaa acactttctt ttcttcagag
     8101 tttttaaata tatctactag tttattcatt attgaagatt taccagcatt tgtatacccg
     8161 actagagcta catttggaag atttgatttt cttctcatac ttctttgagt ttctctttgg
     8221 aattttaaat catccaactc cctatttaaa gatgttatct tttcttctat tcttctacgg
     8281 tcaagttcta attttttctc accagaacct ctatttttag taccaactcc accactttga
     8341 cgtcctaaat tttcatttga acctataagt cttggtaaca tatattttaa acttgctact
     8401 tctacttgaa gtttagcttc tctagttttt gccctttgtg caaatatatc aagtatcaat
     8461 gccgttctat ctataatttc acaattaagc ttttcttcaa gattttttag ttgtgatgta
     8521 gaaagttcat tgtaaaatat aactatctct atattttctt tctttattaa gtttaatatt
     8581 tcctcaactt tacctgtccc aatgtaaaat gctctattaa cctgctttgc attttgagtt
     8641 atactcccta caacatcaat atcacatgct gaacataagt tttcaagttc aatcattagt
     8701 tcattaaagt cctcagattt attatttatg tttaatccta ctgttattcc ttttttcgcc
     8761 atatccatat tctccttatc tgctattttt tatttaatct atagcacgag attatggctc
     8821 tataattaaa ttcatcaaga attttgcttt ttatcttatc cttaaatgac acttcttatt
     8881 tactattata ataaccataa tcgtgcatta ttttctcaat cttatataaa aaatcacttt
     8941 cttacacctc ctcccaaata tattatgcag ttttttcaat aaaaaaactc cttacaaatc
     9001 taagtaatac aaaacaagct acttatatca taagaaggat taaaattcta aatatatatt
     9061 aaaaatacat tagtttaatt aacttatatg taattatcat tttatattta accctattct
     9121 taaatttaag tagttatttg catgattaat aaaagtaata aaaacatata cttcaatata
     9181 atcatcaact attaaaatta aattctattt ataataaaat ttaaagaata aaattaaata
     9241 tacattttga taatcacctc caacattttc cttactacat attataataa aataaacact
     9301 ttgtaaagac ataaaattat gtggctatct caaaatatag agaatcctct ttttcattgt
     9361 taaaatgata aaaatacact ttatttcata cacaaaagag tgccttatga attaaaaagt
     9421 tcattttaag gcaccctcat ttattttatc ttattaatta catgtctatg ctaagatagt
     9481 tttttgttac attgcttcat cttcttcaaa ttgagaattg taaagattgg catagaatcc
     9541 gcctttcttt agaagttcct cgtgatttcc ttgttcaact atatctcctt cattcattac
     9601 aagaatcata tcagcatcac gtatagttga taatctgtgg gctataacaa aacttgttct
     9661 accttccata agattatcca tagctttttg aatcaatact tctgttctag tatcgacaga
     9721 acttgtagct tcatctaata ttaaaatttt aggatcagct aaaatagcac gagctatagt
     9781 caataattgt ttttgacctt gagaaacatt acttgcttct tcatttaatt tcatcttata
     9841 tccatctggt aatgtcttaa taaatctatg aacatgtgca gatttagcag cctctataac
     9901 ttcttcatct gtagcattca acttaccata tcttatattt tccataattg aaccactaaa
     9961 taaccatgta tcttgaagaa ccataccaaa catttctcta agctcactac gattaaaatc
    10021 tttaatatta tgaccatcaa tcagtattga accgctatta acatcataaa atctcattaa
    10081 cagtttaact attgtagttt tacctgctcc tgtaggtcca acaatagcaa ctttttgacc
    10141 aggtttaaca tttactgaga aatcatttat tatagtcttg tttgggttat atccaaagtt
    10201 gacattttca aaatcaattt taccttctaa accatctatt gaaactgcat tttcaacaat
    10261 ttggacttct tcttcctctt ctaaaaactc aaatactctt tcagaagctg ctgctgttga
    10321 ttgcaataaa ttggcaacct gtgcaatctg agtcattggc tgtgtaaagt ttcttacata
    10381 ttgtataaat gactgtatgt caccaacctc aattgttttc ttaattgcaa gccaacctcc
    10441 taagattgat accattacat atcccaagtt tccaataaac atcataagtg gttgcattat
    10501 acctgataaa aattgagatt tccacgctga attatacagg ctatcattta gtttatcaaa
    10561 ctcttcaatt acttcatcct cacgattaaa tgcttttact attgtttggc cactgtaagt
    10621 ttcctcaact tgaccattta catttcctaa atattcttgt tgatatctaa agtatttttg
    10681 tgatttcttt attattcttg aaattacaaa cattgatatt ggtaatatta aaactgccac
    10741 taatgtcata attccactta tagataacat cattatcaaa acacctatca tagttgttac
    10801 tgatgttata agctgtgtca tactttgatt taaactttga tttaaagtat ctatatcatt
    10861 cgtaattctc gataatattt caccatgcgt cttagtatca aaatacttca ttggcaatct
    10921 atccaatttt gctgaaatct cttttcttaa attataggat actttttgag aaattccact
    10981 cattataaat ccttgtataa aagagaacag tgcacttatc aaataaagga aaagtaaaaa
    11041 ggttaagatt tttccaattg cattaaaatc tatacctacg tttcctccag aaactttact
    11101 cactagccct tcaaatatct ttgtagttgc ctttcctaaa atttttggac ctactataga
    11161 aaacgacgca ctaccaatag caaatacaat tactagaatt attgatattt taaatttaga
    11221 taaatatgca attagttttc gcatagtccc tttaaagtca tttgcttttt gtcctgctcc
    11281 catagatttt cccataggac ctcttcccat tctactcatt agctagctcc tcctttgaaa
    11341 gttgtgataa agctatttgc tcataaactt cgcaattttt tagaagctct ttatgtgttc
    11401 ctattccaac tatttcgccc tcatttaaaa caattatctg ttctgcattt aagatagtac
    11461 taattctttg tgcaactatt agaactgtac tttcagcagt ttttgatttt aaagccttac
    11521 gaagagctgc atcagttttt aagtcaagtg ctgaaaaact atcatcaaat atataaactt
    11581 ctggattttt tgctattgct cttgcaatag aaagtctttg tttttgacct ccagatacat
    11641 ttgttccacc ttgagaaatt tcagttttaa aagcttccgg tttagaatct ataaattctg
    11701 tagcttgagc tatttcagct gctgttctca tttcttcatc tgttgcgtct tttctaccat
    11761 attttaagtt ggattctata gtaccagaaa acaatactcc cttttgaggt acatatccaa
    11821 ttttttccct taaatcatgt tgagaaacat tttttatatt aactccacca agtaatattt
    11881 ctccttcagt aacatcaaag aaacgaggaa ttaaatttat aagagttgac ttaccactac
    11941 cagtacttcc tataaaggca gttgtttctc ctggctttgc tgtaaaagat atatttgtta
    12001 atataggttc ttctgcgtct ggatacatga acgaaacatt tttaaattca acaagaccct
    12061 ttttatcttc cttaaactct tgtgcctctg atttttctgg gtcttttatg cttatatctg
    12121 acactaatac ttcatctata cgtgtagctg aaacggatgc tcttggaagc ataatagata
    12181 ccattgatat cattaagaat gacattatta tttgcattgt atattgtata aatgccatca
    12241 tatctccaac ttgcattgct ccagaatcaa cactgtatgc acctctccaa actataagaa
    12301 ctgtgataag gttcattata agcatcatag caggcatcat acatgtcata actctattta
    12361 caaaaagatt tgttttagtt aaatttttat ttgctttatc aaatctttct tcttcatgtt
    12421 tttcagtact aaaagctctg ataactggaa taccagtaat tatttcacgg cttactaagt
    12481 ttaacttatc aacaagcttt tgaaccagtt taaacttagg cataaccact gagaataaca
    12541 ctacaattaa tgataagata gcaactactg ctactgctat aatccaggcc attgaagtat
    12601 ttgtgtttaa aacctttatc actccaccta tagccattat aggtgcatag aatactattc
    12661 taagcaacat aaccatcaac atttgtatct gttgaatatc atttgtacta cgagtaatta
    12721 atgaagctgt agaaaattca ttcatttcag tatttgaaaa tgacataact tttctaaata
    12781 ctccacttct taaatttcta cctacacttg cggctactct tgctgctaaa aatcctactg
    12841 ttattgtagc taccatacta atcattgcta taccaagcat tttagcacca gtaaataata
    12901 tataattatt ttgagttttt tctgtatcaa tacctatttt ctcatattct gaccttacat
    12961 atgaaacaga gctttgtgtt atcatactct ctggcatact ttcaaagcct ttgctcatct
    13021 ctttagtcaa tgtatctaac tgttcctttg gcattgctga taacaattta aatatatcaa
    13081 catctccact ttgagtagct tgtggtggaa gtttgctcat taactgagct ttcatagctt
    13141 ttacctcttt actatcgccc tcaagccctg atactattaa aatagcttta ccaaaaataa
    13201 catttaaatc atctatagtg tctttgtctt tagtatttaa ctcataaaga gattcatctt
    13261 ttagacctgg atacttatct ttatacttaa cataatcctt tttattcagc aaagtataat
    13321 tatctagtac tttatcctta ctacttttat ccataaataa agtaatttta tttaattcac
    13381 tctctctaat cacagatgga acagagtttt ctactccacc ttgttgtata cctacattta
    13441 ctatattgga tgtgtattct ggtaaagaaa ggtcacatac cgcttgaatt accaatagac
    13501 agactattat tactattgat aatgccgatt tctttaaata tttaattaat ttaaacatac
    13561 atttcttccc ttctgtaata ttaattagtc taataacatt aaattgaaaa acattatttt
    13621 aaactttaaa aatatttttc atcttcatgc ttatgacatc ctgcttttaa attatctcgt
    13681 acttgcatta gaagtcttct aagtactatt ttttcttctt cactaaatcc taaaaaaaac
    13741 tctctttcca attccttgtt aaattcttca actttattac atatttctaa acctttgtct
    13801 gtcaaaaaaa tcctagatac tctttggtcc ttttcatctt gttttctttc cacaagtcca
    13861 gctttttcca tcctctttat cataactgta gttgtagatg cttttacttt ccttttatct
    13921 gctaattctt tttgactttg cccatctttc atctttaaag aaaaaagtat ttggggttga
    13981 ccatgatata agcctaattt atcaaaataa aaatgagttc ttataaaata tattcgcatt
    14041 aattgcaaga aaacatcatt taatttttct tcagaattgc tattcatact tgccctcctt
    14101 attttttata ttacttagtc ggctaagcat tattttacta tactgcatgc attttttcaa
    14161 tatatttata aaaaattcat ataaatttca tttattaaat atttttatat catatagtta
    14221 taattttaat aaataatagt aagattttta attagtcagc tacttatatt ttttattgta
    14281 aagcttaaaa taaacctttt catatatttt caaataaaaa agttgtccca aaataaattt
    14341 tgaaacaaca tcatattcaa cagtatcttg attatataac attatgatac tctacttatc
    14401 ttcatatcca tttacaacaa cgtctaactt cccagtttca ggtgatataa ttagaccatg
    14461 tacaataata ccatctggaa ttaaaggatg cccttttatt aaatcaatac tatcttttac
    14521 agattcttct gcagaatcga atccatgtaa ccattgtttt gtatcaattc cagcactacg
    14581 aagtgtaagt attacatcat tagatattcc tctatctaaa attttaccaa gtaacttatc
    14641 cgtatcaaca ttacacatac cacatccatg atgccctaca accataacct catctacatc
    14701 aaattcgtaa attgcaacta aaacacttct cataatactt ccaaaaggat gcattatagt
    14761 agctcctgca ttttttatta gtttcacatc tccatttttc aaattcatag ccttaggcaa
    14821 aagctcagtt aatcttgtat ccatacaaga taagactact atttttttat ttggatgttt
    14881 tgatgtcaca tattgttcat attctttgtt ctctacaaat gatttattat attctaatat
    14941 ctcttcaagt tttctcatat gtaatatcac cctctttaaa aattttttta ttattatatc
    15001 actattaaag tacaatatat acacatataa tttaatttat atttattaaa ttattttata
    15061 agattattac tctactataa atcttgtata taacttgtat ttataagtat ttgtatattt
    15121 attttttata atctaaacta atattatata ttttcattat ttttttaatt ataatttttt
    15181 taaaatttta ttctaaatgc cttacttata attaattttt tatttcacct atatataatt
    15241 agatttaaaa atgtttacct taccaatata atgattaaac tcaaaagtct tattgaaaaa
    15301 ttttctattc acataaattg ttcaatatat aaaatagaaa atttttttaa ttttcaaaat
    15361 atattctaca tatctaatat gtaattacaa taaaaaatgc tataattaac atattattaa
    15421 ttcgttttat tattgtagta tggctatgaa attatgaaaa tatttctcta attaaaaata
    15481 tgatttatcc atatttgttt attgtagata cagactatac aaaaataaat agcacttatt
    15541 tcagtataaa aacatatata gtctatattt agactaaaaa agtataaata gtctatataa
    15601 aacaacttaa actatgaata taatctaaag tggagggata ataattggca aatataggaa
    15661 aaataatagg agatagaata aaagtctata gaacaaaatt gggatatagt caagagtttc
    15721 tagcagaaaa ggcaggttta catccaacat atattggtca gattgaaaga gctgagacaa
    15781 atacatctat caatattata atgaaaattg ctactgcttt agacatacca cttgaattgt
    15841 tatttgcaaa tattattaca acagaaggcg ttgataactc tgtaccttta gaatgttatg
    15901 aaatgattaa taaattaact gaaaaagaac aaattgcaat gctagagtta ataaagtgca
    15961 tcattaaata caaagaaata taagtgaggg aaatagtaac aataatgaat tatataggta
    16021 aaagacttaa agaagaacgg aaaaaagcaa atttaacttc aaaagaattt gcaaatatgg
    16081 ttggagtatc gccttggtat ataactcaaa tcgaaagtgg taaaaagaat ccaagtctta
    16141 aaacattcat aaaatttgta aatatattaa atatttctgc agatgttctt ataaaagata
    16201 ttacttccac tggaaaaaca tatttagaaa atgatataaa tgaagagtta aaagacttaa
    16261 attcaagaga actaaatctg atatcacaga ttatagacat cataaaaaag aataaaatat
    16321 aattctttat attttatata aaaaaaaagg ttatttgaaa taaataacct ttttttgtct
    16381 ttttgtgtta acattgtctt atagtgctaa tgagtactat tagaccatgt tctatggcct
    16441 tattttaata taaaacgatt ggagataggc tatgattgtg attgaaggta gcgataaatt
    16501 taagattgca aaagaatata ttgatgtaga atatactctt tttagcaaag taacttttag
    16561 gtatgaaaag ttgaaattta aagataatgc tgaattggaa aaaattaaga tgtttaaata
    16621 taaaaatggc tacatcccta ataaattaaa cctttctttt ggatatggat tctcttctta
    16681 taaaaagcaa ataattagag aaactgtaga tactttaaga ttgacagaaa ttttttcaag
    16741 cgagaacata gaagatatta aatttataaa agatggtaca aaaaaattag aaattagcat
    16801 agagaaagtt gtgaaattta aacgtcgaaa aaaaagaaat tatgtttgtt gctattgccc
    16861 tgatatgtat agagacataa aactcgacaa agaatctatc aataagatat acaacagaaa
    16921 aataaaaata gaaagagaag ttaatatttt tgaagatgag gatgttataa taaacaagaa
    16981 agtattgaag tttccaaagt cttggacaaa gaatatgcaa aaatattggt taagtgaaaa
    17041 taagtatccc atacattcta ctgtaattga tgatgataga tataaatgtt gtaatgtaca
    17101 atatacaaaa aatagagtga taatattata ttacatatat aaccattaat atacataatt
    17161 acaaaaaaat aatgacaaat atacataata caaatatatc ttctacttta gataaaacag
    17221 ttattatgaa aacttactat ctataaataa tagaaatgct agtataccat ctataatcta
    17281 atgtctagta ggttttaatg gcatatttta aatatgccat agttgattta taagattttc
    17341 tatatcactt tcaaaataca cactaaatag ttgcttttca gatataattg cccaatttaa
    17401 attttactat ctgaaaagtt ttttatattt aatttatatc ttcaaaaata ccgtctaata
    17461 tttctatata attaatttaa ttaatactct tatgtaatag attaagtact gaatttaact
    17521 ctttaaatga tatttgccct ggtgctgaaa cactttttgc agctccaaaa gtcaatgcag
    17581 aaccaaatat ttcaccacac aatctactta taacacccat tcctgacatt gacattgtta
    17641 ttattggtct atctgcataa attttaaaca tttcatttgt agcttcaagt agcactaaca
    17701 catctttttc attttgcggc ataacagcta ttttgggtaa atctgcgccc aattcctgca
    17761 ttctacataa acgagataca atctcttctt tttttggagt tttattaaaa tcatgattag
    17821 atataattac tttcacttct tttttatgag caaaattaac tacttcatct atgacctcat
    17881 ctcccataaa taactctaca tctattaaat ctactaaacc tgtattagaa atttctttat
    17941 tcaaagtagt ataataatct cttgatatta atttttctcc cccttctact acacttctaa
    18001 atgtgaataa taaagggata tcatgtatat aacttcttaa ttcatagaga acttccttaa
    18061 cttctttaat attttctaca ttctcaaaaa aatcaactcg ccactctata atatccaaac
    18121 atgcatcttt taactcttta gcctctttta taatatcttt tttatttttc ccaataattg
    18181 gtacacaaat tttaggtcta ccttctccaa tagtaatatt ttttacttga acttttcttt
    18241 tcatattctc ctccataatc agaaattcta taggacttta aattctattt tctgcacaat
    18301 ttaattatac attaaaaata ctatacatta taaaaatact aatttaaaaa taatttttat
    18361 tctaattctt aaaacaatcc cacatatttt agaaaaggta ctctataata tacaaacact
    18421 tcttttttac ctaaatttat tttaacatcc tataattttc acaatattct ttaaatttta
    18481 ctcatataaa caatattatt ctggatgcta atagaattaa tctataaatc atagtataat
    18541 tatgcagaaa taactcgaat ttcactactt atgaagagat tgaatattaa tgaatctaaa
    18601 tcatttgcat tacttaagag ttttgactaa agttgaacac catacaaaag tagctactca
    18661 attgtttata ccccaaccaa gcttaagtaa tgttatatag attctcgaaa aagaatttgg
    18721 tatcttttta tttgaaaaac ttatattaaa tttgattatc ttttcataag ttggtaaatg
    18781 tcgatatatc ctgtatctac aaggagtatc ggcattttct ttttggaaga tactttgcat
    18841 aagttggcat ttaaccgtat aagaatgcca tcaaaagtag taaataagta gtaaactcta
    18901 gtcatactac taggattata accgttccat ttcagctctc gaagaactaa atgaaccatg
    18961 tgcataatag ccaagcgtca tagtaatgtt tttatgcccc ataatatatt gcaatatatt
    19021 aggactcatt cctttgttag ccatatttgt acagagtgta tgcctaaagg tatgtggcgt
    19081 aatctttggt agcttgtcct catgggtctt attatacttc ttgactaacg ctcttaacat
    19141 gctttgataa tgagttgctt gttttggtaa tccatcttgt ttaagaaata ggaagttctg
    19201 gtaaccatca acaatgattg gttctgcttt agcgttctta cgccgttcca cgatacgttc
    19261 aaatgattga taagctcttt cagtcattgg aatttctcgc ttaccctgtg tggtctttgg
    19321 aacttctaca taatagccaa tcttagtatc actcaacaat tgatggtcta ccataatggc
    19381 tttatttttc atatccaatt ctaaagtcaa accgcataat tcagaaatgc gtagtcctgt
    19441 ttctaagagg ataacaacat catcataata tttacaataa gtcttatcgc tccccataaa
    19501 ggataataag cccatttcct gttctggtgt tagagcgatt tttggttctg ttgtatcttc
    19561 caaaacatca cttaactaaa aattaaatgg attctttcta atacagtcgt cttgaattgc
    19621 catgaaaaac gacgctttca gtgagcgctt ataattgtca atggatttat aagcacaccc
    19681 atcttcactc attcggatag cccagctttt agcgtctgat aatttaacca tatcaatgct
    19741 ttttgcacct agtgggtcat tgatagtatc tatattttga tttataattt atacattttt
    19801 ttaaaaacag acttttaact gtaaagaatc tatgtataaa cttagctatg ttgtccgtgt
    19861 atctacaaag aatcttgacg agctaaaaag gcgttgtgat gaagtcaaag acttttacga
    19921 cgacttatca atcaagctcg tgcgtccctt tggggatatg ttgggattgc atggcgaatt
    19981 tattcctgtc agcaaacgtt acatcaatga ttatattcaa tatgtcactt cggatttttt
    20041 ggctggactt ggttttggag cgacacaaac ccttggcgaa catgaggata tctatcttgg
    20101 ctacaatctg gacactggaa agaatgtcta tctaaaacct gcccttgcta gtcaaggggt
    20161 aaagggttct attaccaatg cgttagcgtc tgcgtttatc ggttcattgg gtggtggtaa
    20221 attattcagt aacaatatgc ttgtttacta tgcggtacta ttcggcggac aggcggtcat
    20281 agttgaccct aaagcagaac gcggcaaatg gaaagaaacg ctaccagaaa tagcccatga
    20341 gataaacatt gtcaatttaa ccagtaatga tgaaaacaaa ggattgcttg acccttacgt
    20401 cattttgagc cgtaaaaagg attctgaaag tttagcgatt gatatcctaa cattccttac
    20461 tggtatctcc agtcgtgaca gtgataagtt tttgcagaaa aatgacaact ggaaaataat
    20521 aatggaatag tttctatgta aatatatttt tactatctta cctaactaat tgaaagatga
    20581 tataatttaa tcaaagattt ggtaaggagg gatgaatatg catttaattg attttatgaa
    20641 tgaagatatt aaaaagaagc ttgttaaaaa aacgtataaa aagaaagaga ctattctttt
    20701 tgctgagcaa gaaaatgaat atgtaattct tatgattgac ggtattgcag aagcattcat
    20761 cttaaatgtg aaaggaaaca tctcaacgat tcatctttat agaagtggta gtttttttgg
    20821 agaaatggaa caatttaatg atgggaaaaa acccgttgaa attgtcgctt tcacagattg
    20881 taatgtatat aaacttcata gagatgattt tttaaactgg ttaaggggag attttgaagc
    20941 tacaaaattt ctgattcgag aaatatctag taaattagtt gtaaatgctg aattaattga
    21001 agaattatca tctttaagtg ttaaagaacg acttctgagg tgtatatcct tacactatta
    21061 tagaagtgaa ttaaatctgc ttaccaaaaa acagcttgct agcgaagtaa acgctccaat
    21121 tagaagcatt aatcgtgcta taaatgaatg tgtacaagaa catctaattg cttatgaaaa
    21181 caaaagattt agtgtaatta atcagcgaga ggtattaaag aatcttccta caacataagt
    21241 ataatttcaa aaaagtattg aataataatt attcaatgct tttttatttc gggtcaagtg
    21301 acctattttt ttttatttct tcattatata atactcctat gcataattat gttggattga
    21361 tagctaataa aagacagtga gaggtgttat attatgacta aaacacaaca gaaaatgata
    21421 caaaacgtat tagaattttc aaaaagagat agtaggataa taggacttgc aattagcagt
    21481 tcttatatca caaaatcgtt agatgaatat tctgatttgg attttctgat tgtagcagat
    21541 gataaatcct atgaagaagt tatcgaagag agattactta ttgtggaaca gtttggaaca
    21601 ttagtttctg cttttactgg cgaacacgtt ggagaacctc gattgataat ttccctctat
    21661 gattcccctg ttcttcatgt tgattttaag tttacaacct tagatggttt actagatagg
    21721 gttgaagatt cagctattct ctatgaagaa aacaactgca taacagaaat ttattctaaa
    21781 aaagaagcat acttcccaac tccagactta cagtggattg aagatagatt ttggatttgg
    21841 gttcattacg cttgcactaa gattggacgt agagaactat ttgaaacaat tgatttttta
    21901 tcttttttaa gacaatcagt acttgcccca ttaatacagc ttctcttagg taaattacct
    21961 cgtggagtta gaaaaattga attggacgca ccccaatata ttgaagcatt agaaaagacg
    22021 gttgctaacc atgaatataa aagctgtgtt aatgcattgc aggctactat caacctgtat
    22081 cttgagttaa gagaggaatt atcacaaagt cctattgtcc gaaaggttga agctgaaagg
    22141 gttgcattac agtattttca agatttaatc aaataaaccc aaagtaaaaa aatattgaat
    22201 agtaaatatt cagtgttttt tcatttttgg tcaaatgacc tagattagtt gttgtaacaa
    22261 ttctataatt attttgaaag taacagtatg gaggataact atgagtgata ttttaagtag
    22321 aattacaaat aaaattgggg ataagaatat agttgataag ttatcagctc tatcaaaatc
    22381 agatttgaac tcacttctat tagaagtgtt tgatagacaa gcaaatactt taactgcaac
    22441 tgatattcta aagtcatacc aactaaatag atttactatc cctagcggta tagatccaga
    22501 agaaattcat gctttggaat caaaattatt gagaaaagca ttcaatatgg atatcaagac
    22561 gattatgctt tctccctctg ccccattagg tagctgtagc gtttttggct ccgtagatca
    22621 atataacgta gtaagtgctc tacgagggac agaaacactt gcagacccct caaatatgtt
    22681 ggcaataatt attgctgata aattaaaaag aaaagaagaa aataatacca tacctatcca
    22741 catggctact actgctaggg taataagagc acaaaatttt gtaggtaaag gtttatattc
    22801 acattttggc ttatattgta tggtttcatc tggtatagat acagggtctt acacttgtga
    22861 aaaaatgttg ttagaaaagc acttaaacta ttataaagat atattaagtg aaatagaaaa
    22921 tgttcgtttc tctattattc tacgcaaaag aaacggatac aaagataatg acggattttt
    22981 cgacagaatg gttgaaacta tcaaacttat ttttcctaat atagagttat caattcataa
    23041 tgatgatgtt gataataatt attataaagg tatcaatttc aaattatcgg tcaaacaagg
    23101 aaatgaaacc gttgaaatag ttgacggtgg atttgttgac tggacttatc aaatgcttgg
    23161 aaataaaaga gaacgctgtt tgataagtgg tattggctta gaaagatttc ttgtagctgt
    23221 ttcataaaag gagaagaaaa atggaactta tagagtggaa ttatgcttat attaccgatt
    23281 tagttgagtt tgcgaatgat aaagaaattg caaagaatct tcgtgacaac ttcccccacc
    23341 cttataggga gcaaaacgct agagattttg ttagtttttg tttggcaact tcagatgtta
    23401 aacaacttag ctatgctatt ttttatcaag aaagggctat tggaagtatc agcctaacat
    23461 ttgaagaaga tacctctatt aataaaaatg ctgaattagg ttactggctt gctaggaact
    23521 attgaggcaa agggatagca acaaatgcag taaaagaaat atgcaaaatt gcctttgaaa
    23581 aatacaacct taacaaaatt catgctaatg tatattccta caatagagag tcaagtaaag
    23641 tattggaaaa atgtggcttt aagattgaag aatactgtca caattcataa taaagaatag
    23701 aaacatgatt gattgccatg tatatagttt aattcgaccg caatagcatt tattagaatt
    23761 aacctacttt ttaataacag aataagtttt tcccctctat taatttctta tgcatctttg
    23821 tatttttatg aaaagctagc caatgcatat ttccatacat caatatggtg gtggcttaca
    23881 accaatataa attaaatacg ttttaaagaa tacacatttt tgccctatag gcagagtgtg
    23941 tatttttttc tctttttttt gaaatttatc gaagatttaa cgccctccca ccgtgatagg
    24001 agtaagcaag gttatttaac tccttgcgat ggaaagaggg tgaaatctta tggttgatga
    24061 aaagacaatt gaacatcaat tcgacagttt ctgtaaaaca gttttgcgta actatgctag
    24121 agatatttat gacgaaaata aacgcagaaa tgattatttg gtatctctcg aatctttgtc
    24181 acttgcagaa ttaagtaaat tgagtatttt agatgattat gattctaact acatctgtat
    24241 ggtttcttac gactacaaca tacgaattga agatgtatta atggcgcaag caatcggaaa
    24301 acttacaaaa agaaaacaag atataattct actttcattc tttttcaaca tgacaaatgc
    24361 tgacatagca acattgatgg atttagcaga aaataccata cattatcaca aaacaaatgc
    24421 actaaaagag ttgaaagaac tcatgcagga acattgaaat gaaacctact aaagaagtat
    24481 taccttttac aattatttta cttgctgtac atggcgatgt aaatgcaata aacgtaatac
    24541 ttaaacattt tgaacactat atcattcgtc tttcacagaa aactttattt gatgaatacg
    24601 gaaatcccta tattcatgta gaagaagaaa caaagcgaat gctaggaaca aaactaatca
    24661 ctgctatttt gagatttaat ttgtcttaat ctcgccctct ctccatgtat caagctattt
    24721 ggtacatgga gaatcactaa ttatttgtct ataaaagccc acaagctatt gtgatttttt
    24781 ataggcaaat caatgccgac tgttctttga caactgaata aagtagatag atacgtttgt
    24841 gatacagata gccaacaaat actatacgcc atgaccttaa tcaagagagc tactatatag
    24901 tgatttgtga ataacctttg gaacggttgt tttgccgtaa cctgtaccac agtataatga
    24961 tacacctgta tgacacggtt ctgtccataa gaatgggatt ggttgaaata ccaatggagc
    25021 ttatcaaagc catctatcta cttctgaact acgaacatag aaaggagtga tacattatat
    25081 gaacaatact gatgtaccta tttgggaaaa atataccttg acgattgaag aagcgtcaaa
    25141 atactttcgt attggagaga aaaaattacg taaacgtgct gatgaaaacc ttgattctgg
    25201 ctgggtcatt atgaatagca atcgcattca aataaagaga aaacaatttg agaaaatcgt
    25261 tgatacactt gacgttatct aatgaaattg agccttatat gttataatat tcatatgacg
    25321 tatcaagctc aattcggtta ttgagaggag cttcttatca tgtcaactga aaaaagacga
    25381 gataataaag atagaatttt aagaactgga gagagccaga gaaaagatgg aagatacgca
    25441 tataagtatg tatattcatt tggtaaacca caatttgttt actcttggaa acttgtacct
    25501 actgataaac ccccaaaggg aaaacgtgat gatatatcat taagggataa ggaaaaagca
    25561 atacaaaatg accttgatga tagcattgac actattggta agaaaatgac agtttgtcag
    25621 ttatacgcta aaaagaatgg actaagaaag aatatccgac ttaacactaa aaaaggacgc
    25681 cattatctta tgaagattct taacaaggag tgattctgtg aagagatgta ttgtagtagt
    25741 ggaatagtca aaaacataat atatattaaa ggagagattt aaatatgact attccaagtt
    25801 caaataaggt atatccaaga agtaatgatt atcaaactgt atatcttaaa aatgtaataa
    25861 caagggaaaa tatagaagtt ggagattaca caatatataa tgacttttat aatgacccaa
    25921 gagattttga gaaaaacaat gtattgtatc attaccctat aaacaatgat aaattaataa
    25981 ttggtaagtt ttgctcaata gcatgtaatg caaaatttct aatgactagt ggaaatcact
    26041 caatgaaatc aaaatccaca tatacttttc ccatctttta tgaagaatgg aaactcgata
    26101 tcaaccatat aacggacgct tgggacaata agggtaacat tataattgga aatgatgtgt
    26161 ggataggtta tgattctata attatgtcag gtgtaaatat tggtgatggt gcaattattg
    26221 gaacaagagc agtagttact aatgacgttc caccgtattc tattgttgga ggagtacctg
    26281 caaaaattat aaaaaagaga tttaataatg atacaatttc aaaattatta gaaattaaat
    26341 ggtgggattg gccatataaa aaaattcaat caaacattaa gcatattcaa tcaggaaata
    26401 ttgataaatt agtttaatat tttataattt atttatatta aactttactt taataatcat
    26461 tttataag
//
LOCUS       DAMJVK010000265        17179 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.guided.088, whole genome shotgun sequence.
ACCESSION   DAMJVK010000265 DAMJVK010000000
VERSION     DAMJVK010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17179
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.guided.088"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(240..764)
                     /locus_tag="PC414_003932"
     CDS             complement(240..764)
                     /locus_tag="PC414_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF2466223.1"
                     /translation="MENKNPIVTIEMENGKEIKIELYPNIAPNTVKNFVSLVNEGYYN
                     GIIFHRVIPGFMIQGGCPNGTGMGGPGHSIKGEFSGNGFTNNLKHERGVISMARTMAP
                     NSAGSQFFIMHKNSPHLDGQYAGFGRVIEGMDTVDEIASVRTDSADKPQTPQVMKSVT
                     VETFGIDYSDVEKN"
     gene            complement(829..1581)
                     /locus_tag="PC414_003933"
     CDS             complement(829..1581)
                     /locus_tag="PC414_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDF2466224.1"
                     /translation="MKLGLCLEGGGAKGAYQAGVVKALYDGGINKFYSISGTSIGAIN
                     GYYLYTGNVDNLEKMWTNIKDIQNGNVKIVNNTVDNSPAIDNLRELDDSNIEEMNFYV
                     NYVEVDNKVVSEKIVDVSKMPRNEAIISISYSGLLPSNPNATLGFKEQFVKDVQEGIY
                     DGFKLDGGLIRSALIEPLIGDNVDKIILISTKYNYELPEDIKKVYDEDKIIVVRPNTQ
                     FAPKDTLNFDDEFCKTIYQEGYEIGKNILDRL"
     gene            complement(1612..2910)
                     /locus_tag="PC414_003934"
     CDS             complement(1612..2910)
                     /locus_tag="PC414_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HDF2466225.1"
                     /translation="MDSINFAKNLIDFIYDSPSSFHAVESTKEILDKNGFEELVLNQR
                     WNLRVGGKYYVTKNLSAIVAFVVNSEGIERDGFRIIGSHSDSPTFSIKPNAEIEAEKS
                     YLKLNTECYGGAILSTWLDRPLGIAGRVVLKGDSILKPNERLIDFKKPICIIPNLAIH
                     LNRSINDGHSYNKQKDMLPLVGMLNDTLEKDNFLIKQIASNLDVNMDDIIDFDLFLYE
                     FEKGSLIGANDEFISIGRQDNLAMVHASLNALVNTKGQHGVNVMAVFDNEEVGSSTKQ
                     GADSNMLLNILERICISMGKDREGFFESIYSSFMISSDLAHAIHPNIPEKHDPTNKPI
                     MGKGPVIKINASQAYTSDGYSIAIYKNICREANVEYQEFVNRSDERGGSTIGPISSTH
                     IDIPSVDVGSPILAMHSIRELGNVEDHYSIYKTFSKFFEI"
     gene            complement(2927..5191)
                     /locus_tag="PC414_003935"
     CDS             complement(2927..5191)
                     /locus_tag="PC414_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HDF2466226.1"
                     /translation="MNLDTLNPAQREAVEKTEGPVLILAGAGSGKTKVLTTRIAYLIE
                     DKGVQAPNILAITFTNKAANEMRERVEQNIGPETKDMWISTFHSCCVRILRKDINKIG
                     YNRSFVIYDSADQVTLVKDCLKELNLSDKVFEPKAVISAISGAKDKLYTPKQFKDINM
                     ADNRMVKIADIYALYQDRLKRNSALDFDDLILKTVELFKANDEVLAYYRSRFRYIMVD
                     EYQDTSKAQYELIKLLAREHQNICVVGDDDQSIYGWRGADIRNILEFEKDYDNVHVVK
                     LEQNYRSTQVILDAANKVISNNIERKRKKLWSEKKEGELIKIQLTGSEIEEADFIADS
                     IAQIARKENRPYKDFAVLYRANAQARPVEDALNRSQIPYNIYGGTKFYERKEIKDLLA
                     YLRVIQNPQDDISIKRIINVPRRGIGLRTIEKIEDRANLKQESIYSVLIDIETNSDIS
                     TKARASISGFVDIIGTLRTIKEVYPVSKLIEKVLDTTGYMDELVEIRNKNEKDLTGKG
                     EEAQDRIDNLREFISIALEFESSNDDTYENKDLETFLTSIALTSESNDEEDNDRVSLM
                     TIHTSKGLEFPVVFLTGMEEGLFPISRAIKSMSDSQIEEERRLCYVGITRAKEELYMS
                     LTEKRTLYGKTNVAIASRFMEELPEECIERLYKVKKELSYSKASYNMLDKYTKKYMST
                     ISKSKVADKVNATIKDSNKETNPDDIKLGSKVHHPKFGVGTVVSIIGTDVTIAFDQQG
                     IKKINKEYTTLNIL"
     gene            complement(5446..6264)
                     /locus_tag="PC414_003936"
     CDS             complement(5446..6264)
                     /locus_tag="PC414_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDF2466227.1"
                     /translation="MFKINNLTYQYEKNTNALLNINMDFSKGNVIGIIGSNGSGKSTL
                     FMNLMGILKPTSGEILFKEEKLKYDKRSLYNLRKNVGIVFQDPEKQIFYSKVYDDIAF
                     AMRNIGMDEKTIKERINKALVAVNGIDFIDRPVHFLSYGQKKRVAIASVIAMENEIVL
                     LDEPTAGLDPVSTRSIVDIIKGLNKNNIKIVISSHDMNLMYEICDYIYVLDKGILIDE
                     GKAENVFINENNIIQAGLESPWLVKVHRNMNLPLFKKEEDLYKYWKERELNTNK"
     gene            complement(6257..6955)
                     /gene="cbiQ"
                     /locus_tag="PC414_003937"
     CDS             complement(6257..6955)
                     /gene="cbiQ"
                     /locus_tag="PC414_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HDF2466228.1"
                     /translation="MLIIDKYAYTNRLSKVNPNKKVAIGVIFLIASMVIQNIFILSGI
                     MILMSILVVCVAGIDLKNYLKLLRIPMYFLFLSIGITLVNISFNKADLLYSFEFFSFN
                     VGISKASINMSIHVLFRAMSCLTCVYFCILTTPFNQLIFFFKKLHLPDTFVELSMLIY
                     RFIFIFLEEFSEIYKSQELRFGYINLKTSYKSLGILGSMLYKRLMTRYDDMCISLDIK
                     LYDGKFHIVGDNDV"
     gene            complement(6958..7242)
                     /locus_tag="PC414_003938"
     CDS             complement(6958..7242)
                     /locus_tag="PC414_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2466229.1"
                     /translation="MSAKTKTKTNILLLLLVVALIIFPLLVNSGAEYGGADGQAESEI
                     TKINPNYKPWFSSPYEPPSGEIESLLFSAQAALGAGVIGYILGLQKGKRS"
     gene            complement(7232..7984)
                     /locus_tag="PC414_003939"
     CDS             complement(7232..7984)
                     /locus_tag="PC414_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HDF2466230.1"
                     /translation="MKQNIKLGVIAALMLIILTPVTSNAMHIMEGYLPVKWSIAWGVI
                     FIPFFLFGLKSIGKIVKQDPKKKVLLALCGAFVFVLSALKIPSVTGSCSHPTGVGLGA
                     IMFGPSVMFVLGTIVLIFQALLLAHGGITTLGANAFSMAIIGPIVSFLIFKALKKKDG
                     NNAIPVFLAAAIGDLATYTITSIQLALAFPDPSGGVMASAIKFLGIFFMTQIPIAIAE
                     GILTVIVYNLITENGEKSILENNDKGVKANEC"
     regulatory      complement(8166..8358)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(8490..10262)
                     /locus_tag="PC414_003940"
     CDS             complement(8490..10262)
                     /locus_tag="PC414_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation-like protein"
                     /protein_id="HDF2466231.1"
                     /translation="MKWIYDDYEFENRANNLSWTIAGDYNESIDISEKDYSSKEIGLY
                     FAIIAGARRKYVDWDIVKKYLTNRIRKGYNKDVILGLIQVILNTIVEEKVIKDRPGIE
                     DIRRNAYKDIVSRFTKIHNDNIMDKIKYTFVLYSMDKHPALDSMTRRIVNDIRNIDTN
                     QDIMEILKALDVIYLTYFEYILNGEENSLIDENNVESVEVNFDTFADFMYEELYSDEE
                     ENIIESDIDSISSSMLVEGVGDFDDSKCSNSADRVIYVDEDTANKIYSKIEHYYGKTY
                     LSKGEIKKIETQNCRNVHEGCRIHFTDGVLRSECNNVFQLKYVTRQKENNLGKFREHA
                     RMYRQQIKRLKESLSRILIEENAKSRVYSDCGTILANRAWRVGRSNNNKIFYKDIENE
                     KGKYVIDILLDASGSQSRNQGNVAIQAYIIANALTEVGIPNRVMGFSSFLDYTILKRF
                     KDYDDSIKNSENIFEYFCAGNNRDGLAIKSICSGLINRDEENKILIVLSDGRPNDVKI
                     GKSSERTLRGEKAYRGIVGLRDTANEVRKARKQNILVLGVFTGKESELEAERLIYGND
                     FIYSRDITRFSDVVSMYLKKIIRN"
     gene            complement(10276..11205)
                     /locus_tag="PC414_003941"
     CDS             complement(10276..11205)
                     /locus_tag="PC414_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2466232.1"
                     /translation="MDIIDNLRVQGVDEKLIEDVLYFRNYYGLEKDLEYRVTKSKTYF
                     YGKDILSMCIAAILEEENILLSGPKATGKNLLADNLGEIFNRPQWNTSFHINTDSSTL
                     IGTDTFIDNEVKLRRGSVYECAINGGFGVFDEINMAKNDAIVVVHSALDYRRIIDVPG
                     YERVNLHPATRFIGTMNYEYAGTKELNEALVSRFMVIDIPPIEEDKLMMILKNEFSDA
                     DEEKLIHFAGIFLDLQLKSQNGEISSKAIDLRGLMASLKTIRRGLKPTLAINMGLTGK
                     TFDVYEKEMVGDVIKTRIPNKWESIDVFPISHI"
     gene            11426..11611
                     /locus_tag="PC414_003942"
     CDS             11426..11611
                     /locus_tag="PC414_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDF2466233.1"
                     /translation="MKYGCPEVAKKWGISERRVQKLCEESRIPGVSKLGYMWLIPKNA
                     EKPVDGRTKRRKEVQYE"
     gene            11604..12305
                     /locus_tag="PC414_003943"
     CDS             11604..12305
                     /locus_tag="PC414_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2466234.1"
                     /translation="MNSVLIIDDDKELCALMKKCVEQENLSAITAHCGAEGLHLVDKN
                     RHTLSLVILDIMMPDIDGFQVLKKIRETSNIPVIMLTAKNDEDDKVSGLRLGADDYLT
                     KPFSINELMARVNSLIRRYTMLNPAFITDTDCIIINGMIIDKTNRIVSVNNIPVQLTS
                     KEFDLLAFLASNKGRVFTKKQIYTQVWEEEYAFDDSNIMSFISKLRKKIEPDPNHPFY
                     ILTVRGVGYRFNKEA"
     gene            12307..13230
                     /locus_tag="PC414_003944"
     CDS             12307..13230
                     /locus_tag="PC414_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2466235.1"
                     /translation="MNTSVYLLLILVLSLIIIAYLVSVLLRVRIQLTLIKDALEDIKN
                     GNLNRRILTDENDITKQICYDINEIAINSQFQLIQQKQSEQAYKQLMTSLSHDVKTPL
                     ASLVGYLEAVESKIVVGEEKDEYIHVASNKAHYLKNFVENLFEWVKLDSKEQVFHFDI
                     FDLNELSRNIISDWIPVLESSHFEYEFDIPEIEYFLRIDANAYTRIINNLLQNIITHS
                     SGNKMTLRIFENKEQAQIIITDNGKGISSDNLPHIFERLYQCDHSRASKGNGLGLAIA
                     KELINAHKGNITANSTPGMGTEFTILLPKAL"
     gene            13387..14310
                     /locus_tag="PC414_003945"
     CDS             13387..14310
                     /locus_tag="PC414_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2466236.1"
                     /translation="MNDFVIETKQLTKIYGNQTVVDKVNLHAKKGRIYGLLGRNGAGK
                     TTIMKMILGLTSITYGEVDVFGENIKGREKQVYPRIGAIIETPGFYPNLTGTENLEIF
                     AKLRGTAAPNSVKRALEIVGLPYKNKKLFSEYSLGMKQRLGIANAILHDPELLVLDEP
                     TNGLDPIGIAEMRKFIRDLSVEHGKTILISSHILSEISLLADDIGIIDHGVLLEESSM
                     SELEKKNSKYILLQVSDIPKTSLILERQFNTKDYSVQDDYTLHLYNTNLDMGEINKAL
                     VMQNVTVISSQLCNDTLEDYFKKITGGEGIA"
     gene            14303..15040
                     /locus_tag="PC414_003946"
     CDS             14303..15040
                     /locus_tag="PC414_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2466237.1"
                     /translation="MLKLIQTEFLKLRRRKLIWLMLLSALIMPFFALLYFNYFGKTGV
                     EPIKFYKWSAFGYTLWIILPVVLGIFCTMLIHEENQYDMLKQLWIVPISKMGYFFSKF
                     FVVLIYSICFMLITAVASVLFSVLSGYVVFSWASILYLLKKCLEIGVITAFVMLPILA
                     IATSQKGYILPICITLVYAFSGFVIMTINMYLHPLSSMGVIIMRNKDIPGLIFSQEIN
                     VPLAFLCICIWSIVSVLFANTALGRRK"
     gene            15051..15824
                     /locus_tag="PC414_003947"
     CDS             15051..15824
                     /locus_tag="PC414_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2466238.1"
                     /translation="MWKLLWAECQKIRRSKIVWITVFASIMIAVIVFAGGQDVYNDPD
                     LHYGLKSVYDGSRYIDNAGWYMDAVQPWSTFFVLPAVIALLGSYIICREEEEDTIKSL
                     RLIPINETKLTIAKMIITFIFSILLYLLLFTITFLTESVLHFSDLSTKLVLSCMKEYF
                     LDGIGVFFAISPIIALVSRMKKGYWLALVFTEVYSIAGLFAGMSNVLQTFFPIAAIFN
                     ISGYYITTGEKIMGSIISLLLCACLSAFILKGLKHNEKN"
     gene            complement(16365..16973)
                     /locus_tag="PC414_003948"
     CDS             complement(16365..16973)
                     /locus_tag="PC414_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDF2466239.1"
                     /translation="MSVINSFILQYGLISVFVLIMIEYACFPLPSEVVLPLCGAIAAR
                     NHFGFLTILILSIIAGILGSIFCYTVGNWGGKSIINKIIEICPKAKKGIFASQDYFNK
                     YSSISVCVCRLIPLCRTYISFIAGIAGQNIITFVISSIVGITIWNTSLISIGYIFSES
                     WVRIMSHYNDYKFLVLIIPISIIFIGFVIKSFIHKKHHKIRL"
BASE COUNT         5548 a         2971 c         2164 g         6496 t
ORIGIN      
        1 tatttattta atacattatt atttaaaaat aaatatataa acaatcataa taaaattttt
       61 ttaataaaac tttatttttt agttaaaatc atacaatata tagatttcca caatacaaat
      121 aaattaaaaa gtgaaatgta tttgtctaaa aacataataa catttcactt tatcctcatt
      181 tttaagtaat atatattttt aatttttatt tataactaaa tctagttttt atataaattt
      241 tagttttttt ctacatctga gtaatctatt ccaaaagttt ctacagtaac agatttcata
      301 acttgtggag tttgtggctt atcagctgaa tctgttctaa ctgatgctat ttcatcaact
      361 gtatccatac cttctatcac tctaccaaaa cctgcatact gaccatctaa gtgtggtgag
      421 tttttatgca ttatgaaaaa ttgagaacca gctgaattag gtgccatagt tctagccatt
      481 gatatgactc ctctttcatg ttttaaattg ttagtaaatc cattaccact aaattcacct
      541 tttattgagt gacctggacc acccattcct gtaccatttg ggcatcctcc ttgtatcata
      601 aatcctggta ttactctatg aaatattatt ccattgtaat atccttcatt tactaatgat
      661 acaaagtttt taactgtatt tggagctata tttggatata actcaatttt gatttctttt
      721 ccattttcca tttctatagt tactatagga tttttatttt ccataattta ttaccttcct
      781 tttcattagt taaattacat aattacattt attatattat actctaaatt aaagtctatc
      841 aagtatattt tttcctattt catatccttc ttgataaata gttttacaaa actcatcatc
      901 aaagttaagc gtgtctttag gagcaaattg agtattaggt ctaactacta ttattttatc
      961 ttcatcatat acttttttaa tatcttctgg caactcataa ttatattttg tggatattaa
     1021 aataatttta tccacattat ccccaattaa tggttcaatt aatgcactcc taattaaacc
     1081 tccatctaat ttgaaaccat cataaattcc ttcttgcaca tctttcacaa attgttcttt
     1141 aaatccaaga gttgcgtttg gatttgatgg taacaaccca ctgtatgaaa tgctgataat
     1201 ggcttcatta cgaggcattt ttgatacgtc aacaatcttt tctgatacta ctttattatc
     1261 aacttcaaca taattaacat aaaagttcat ttcttctata ttactatcat ctagttccct
     1321 taggttgtct atagcaggag aattatccac agtattattt actattttta catttccatt
     1381 ctggatatct tttatatttg tccacatttt ctccaagtta tcaacattcc ctgtgtacaa
     1441 ataatatcca tttattgctc ctattgatgt ccctgaaatc gaataaaatt tatttattcc
     1501 tccatcatac aaggctttaa ctactcctgc ttgataagcc ccttttgctc ctccaccttc
     1561 taaacataat cctaatttca tagtctcatc tccaaattgt ttatcttata tttatatttc
     1621 aaaaaactta ctaaatgttt tatatatact atagtggtcc tcaacatttc ctaattctct
     1681 tatagaatgc attgctaaaa ttggacttcc cacatccaca gatggtatat ctatatgagt
     1741 agaagatatt ggacctatag tacttcctcc tctttcatct gacctattaa caaattcttg
     1801 atactcaaca ttagcttctc tacatatatt tttataaata gctatagaat atccatcact
     1861 agtatatgcc tgactagcat ttatctttat aaccggacct tttcccatta ttggtttatt
     1921 agttgggtca tgcttttctg gaatgtttgg atgaatagca tgagctaaat ctgatgatat
     1981 cataaaagat gaataaatag actcaaagaa tccctctcta tccttaccca ttgatatgca
     2041 aattctctcc aatatattta acaacatgtt tgaatctgca ccttgtttag tagagctacc
     2101 aacttcttca ttgtcaaata cagccattac atttacacca tgttgacctt tagtattaac
     2161 aagagcattc aaactagcat gtaccattgc aagattatct tgtctcccaa tagatataaa
     2221 ttcatcatta gcccctatta aacttccttt ttcaaattca tataaaaata aatcaaaatc
     2281 tataatatcg tccatattta catctaagtt agatgctatt tgctttataa gaaaattatc
     2341 tttttctaat gtatcattta acatcccaac taaaggtaac atatcttttt gcttattata
     2401 tgagtgacca tcatttatac ttctatttaa atgtattgct agatttggta ttatacaaat
     2461 tggcttttta aagtctataa gcctttcatt tggttttaat atactatcac cttttaaaac
     2521 tactctacca gctatcccaa gaggtctatc caaccatgta cttaatattg ctcctccata
     2581 gcattctgta tttaacttca aataagattt ttcagcttct atttctgcat ttggtttaat
     2641 actaaatgta ggagaatctg aatgagagcc tattattcta aatccatctc tttctatacc
     2701 ttctgaattt actacaaaag ctactatagc tgataaattc ttagtaacat aatactttcc
     2761 accaactctt agattccatc tttgattcaa aactaattct tcaaatccat ttttatctaa
     2821 gatttcctta gtactctcga ctgcatgaaa agacgacgga ctatcatata tgaagtctat
     2881 taagttttta gcaaagttta tactatccat tcctcattta cctccattat aaaatattta
     2941 gtgttgtata ttctttattt atttttttaa ttccctgttg gtcaaaagct atagtaacat
     3001 ctgtaccaat tatagaaacc actgtcccaa ccccaaattt aggatgatgt accttagaac
     3061 caagttttat atcatctgga ttagtttctt tattagaatc ttttattgta gcattaactt
     3121 tatctgcaac tttagatttg cttatagtac tcatatactt tttagtatat ttatctaaca
     3181 tattataact tgctttagaa tagcttagtt ctttttttac tttgtacaat ctttctatac
     3241 attcttctgg cagttcttcc ataaatcttg aagctattgc aacatttgtt ttaccatata
     3301 atgttctctt ctctgtcaat gacatatata gctcttcttt agctctagtt ataccaacat
     3361 aacaaagtct tctctcttct tctatttgtg aatcactcat agattttata gctcttgata
     3421 taggaaataa cccttcttcc atacctgtta aaaacacaac agggaactct aaacctttgg
     3481 atgtatgaat tgtcataagc gaaaccctat cattgtcttc ttcatcattt gattcagatg
     3541 taagtgctat acttgttagg aatgtctcta agtctttatt ttcataagta tcatcattac
     3601 tactttcaaa ctccaaagct atagaaataa attctctcaa attatcaatt ctgtcttgag
     3661 cttcttcacc tttgcctgtt aaatcttttt catttttatt tcttatctct acaagctcgt
     3721 ccatatatcc tgtagtatcc aagacttttt ctataagttt gctaacagga taaacttctt
     3781 ttatagttct aagagtccct ataatatcaa caaatccact tatacttgcc cttgccttag
     3841 tagatatatc tgaatttgtt tctatatcaa ttaatactga gtatatactt tcctgtttta
     3901 agttagctct atcttcaatt ttttctatag ttctaagccc tatacctctt ctaggaacat
     3961 ttataatcct ttttatagat atatcatctt gtggattttg aataactctt aaatacgcca
     4021 acaaatcctt tatttctttt ctctcataga attttgtacc accatatata ttgtatggaa
     4081 tttgacttct atttaaagcg tcctctacag gtcttgcctg tgcatttgct ctataaagta
     4141 ctgcaaaatc cttataaggt ctattttctt ttctagctat ttgtgcaatt gaatcagcaa
     4201 tgaaatcagc ctcttctatt tcactaccag ttaattgtat ctttataagt tctccttctt
     4261 ttttttcact ccaaagcttt tttctttttc tttctatatt attagatata actttattag
     4321 ctgcatctaa tataacttgt gtagacctat aattttgttc caactttaca acatgtacat
     4381 tgtcataatc tttctcaaat tcaagtatgt ttcttatatc tgcacctctc catccataaa
     4441 tactttggtc atcatctcca acaacacaaa tattttgatg ttctcttgct aataatttta
     4501 tcaattcata ttgagcttta cttgtatctt ggtactcatc taccattatg tatctaaatc
     4561 tacttctgta atatgctaac acttcatcat tagccttgaa aagttctaca gttttaagta
     4621 ttaaatcatc aaagtcaagt gcactatttc tttttaaacg gtcttgatat agcgcataaa
     4681 tatctgcaat cttaaccatt ctattatctg ccatattgat atctttaaac tgctttggag
     4741 tataaagttt gtcttttgct cctgaaattg cacttataac tgcttttggt tcaaacactt
     4801 tatcacttaa atttaattct tttagacagt ctttaaccaa tgtaacttgg tctgctgaat
     4861 cataaattac aaaacttcta ttgtaaccaa tcttatttat atcttttctt agtatacgaa
     4921 cacagcaaga atggaatgta cttatccaca tatccttagt ttcaggacct atattttgtt
     4981 caactctctc tctcatttca tttgcagctt tgtttgtaaa tgttatagct aatatatttg
     5041 gtgcttgtac acctttatct tctataagat atgctattct tgttgttaat accttagttt
     5101 tacctgaacc agcaccagcc agtataagta ctggaccttc agtcttttct actgcttctc
     5161 tttgagcagg atttaatgta tctaaattca tatttctcct cctgttaaca gccataaata
     5221 tattttaatc taaaatatga attacttcaa attataatta tgtattatat taatagatta
     5281 tttccaatat attacaatta aataacattt ttccatataa tttatttcat tgtttaataa
     5341 aaaaaataac cttatgattt ggggaatcat aaggcacctt taaggggtct aggttaaggg
     5401 ttattatata taaatctatt tttttaaaat aggtttaggg cttaattatt tatttgtatt
     5461 aagttctctt tccttccaat acttatacaa atcttcttct ttcttaaata agggtaaatt
     5521 catatttcta tgtactttta ctaaccatgg ggattctagg ccagcttgta taatattatt
     5581 ttcatttata aacacatttt ctgcttttcc ttcatcaatc aaaatcccct tatccaatac
     5641 atatatgtaa tcacatattt catacattaa attcatatca tgactggata ttactatttt
     5701 tatattattt ttatttaatc cctttattat atctacaata gacctagtac ttactgggtc
     5761 taaaccagct gtaggttcat caagtaatac aatttcattt tccattgcta taactgatgc
     5821 tattgccact ctttttttct gtccatagct aagaaaatgt actggcctgt ctataaaatc
     5881 tattccatta acagctacta gagctttgtt tattctctct ttaattgttt tttcatccat
     5941 accaatgttt ctcattgcaa aggctatatc atcatatact tttgaataaa aaatttgttt
     6001 ttctgggtct tggaagacaa tacctacatt ttttctaagg ttgtataaac ttctcttatc
     6061 atattttaac ttttcttctt taaaaagaat ttctccagat gtaggcttta atatacccat
     6121 taaattcata aataatgttg attttcctga accatttgaa cctattatac ctataacatt
     6181 tccctttgaa aaatccatat taatgtttaa tagtgcattg gtattttttt catattgata
     6241 cgttaaatta tttattttaa acatcattat cccctactat gtgaaattta ccatcataca
     6301 attttatgtc taatgatata cacatatcat cataccttgt cataagtctt ttgtacagca
     6361 tacttccaag aatacctaag gatttatatg aagtttttaa atttatatat ccaaatctta
     6421 gctcttgtga cttgtatatt tctgaaaatt cttctaaaaa tatgaatata aacctatata
     6481 ttaacattga taactcgaca aaagtgtcag gtaaatgaag ttttttaaaa aagaatatta
     6541 attgattaaa aggtgttgta agtatacaaa aatacacaca agtcaaacaa gacatcgctc
     6601 taaataacac atgaatagac atgttaatag acgcctttga tattcctaca ttaaaactaa
     6661 aaaattcaaa actatataat aaatctgctt tattaaaaga tatatttaca agagttattc
     6721 ctatactaag aaataaaaag tacattggta ttctaagtaa ttttaaataa ttttttaaat
     6781 ctattccagc cacacaaaca accaaaatac tcattaaaat cataattcca cttaaaataa
     6841 atatattttg tattaccata gaagcaatca gaaaaattac tcctatggct acttttttgt
     6901 ttggatttac tttactcaat ctatttgtat atgcatattt atcaattata agcatgatta
     6961 acttctcttt cctttttgaa gacctaatat gtatcctatt acaccagccc ctaaagctgc
     7021 ttgtgcagag aataataaac tttctatttc tccacttggt ggctcatatg gactgctaaa
     7081 ccatggtttg taatttggat ttatcttagt aatttcactt tcagcttgac catctgctcc
     7141 tccatattca gcacctgagt tgacaagtaa tgggaatata ataagagcta ctactaataa
     7201 aagtaataat atgttagttt ttgtttttgt tttagcactc attagcttta actcctttat
     7261 cattattttc tagtatgctt ttttctccgt tttctgtaat taaattatat acaattacag
     7321 taagtatacc ttctgctatt gctattggta tttgagtcat aaagaaaatt cctaaaaatt
     7381 taattgctga agccattacc ccacctgatg ggtctgggaa agccagtgct aattgtattg
     7441 aagttattgt atatgtggct aagtctccta ttgctgctgc aagaaatact ggaatagcat
     7501 tatttccatc ttttttcttt aaagccttaa atattaggaa tgatacgatt ggccctatta
     7561 tcgccattga aaaagcatta gctcctaaag tagttattcc tccatgtgct aataacaatg
     7621 cttggaatat taaaactata gttccaagaa caaacattac gcttggtcca aacattattg
     7681 ctccaagacc tacaccagta ggatgtgaac aacttcctgt tactgaaggt atttttagtg
     7741 ctgataatac aaacacaaat gctccacata aagctaataa tactttcttt ttagggtctt
     7801 gctttactat tttacctatt gattttaatc cgaataagaa aaatggtata aatataactc
     7861 cccatgctat agaccatttt actggtaagt aaccttccat tatatgcatt gcatttgatg
     7921 taactggagt taaaatgatt aacataagtg cagctataac ccctaattta atattctgtt
     7981 tcatcttagt atccctctct ccctttctaa taattaaaac ataataatac atgtctgatt
     8041 tatttaataa aataaaaaag cctaactcga tggttaggct tctaattttc cacaatctaa
     8101 atacaaacac ataaaacaaa catatctgta taattattat aagaaacttt ctccatcccc
     8161 gaagaaatta ctttaatatt ttaggcaggt ctcctgactt agcttcatcc tactcctttt
     8221 ccttcccatg agatataaaa aatatcatca tagtggctaa caaaagtttc gtctgcatca
     8281 cagtagcggg ggctgtaaag gaatttaacc tttttcccta ttaatcttgc ttaaagaacc
     8341 tataatatta tatatttaat ttttaaatta ttcttaaatc attttctata taaatcattg
     8401 tatacctttt ctgtgaaaat ttctacattt tttataaatt ttattcattt ttatattttt
     8461 atgtatataa attctttttt gtaagtttat taatttctaa ttatcttctt taaatacatt
     8521 gaaactacat cagaaaatct agttatatct cttgaatata taaaatcatt tccatatatt
     8581 aatctttcag cttcaagttc tgattctttt cctgtaaata ctcctaatac taatatattt
     8641 tgctttctag cttttctcac ttcattggct gtatctctca aaccaactat acccctataa
     8701 gccttttctc ctcttaatgt tctctcacta ctttttccta tctttacatc atttggtctt
     8761 ccatcgctga gaactatcag gattttattt tcttcatctc tatttattag accactacat
     8821 atagatttta ttgccaaacc atctctatta ttaccagcac aaaaatattc aaatatattt
     8881 tcactatttt ttatactatc atcataatct ttaaatcttt ttaatattgt atagtctaaa
     8941 aaactagaaa atcccatcac tctatttggt atgccaactt ctgttagagc atttgcaatt
     9001 atgtaagctt gaattgccac atttccctga tttctacttt gagagccact tgcatccaat
     9061 aatatatcaa taacgtattt ccctttttca ttttctatat ctttataaaa tattttatta
     9121 ttattacttc taccaactct ccaagctcta ttggctaata tagtaccaca atcagaatat
     9181 actctacttt tagcattttc ttctattaaa attcttgaca gactttcttt taatctcttt
     9241 atttgctgtc tatacattct agcatgttct ctaaattttc ctaaattatt ttctttttgt
     9301 cttgttacat attttagttg aaatacatta ttacactcac ttctaagaac tccatctgta
     9361 aagtgtattc tacaaccttc atgaacattt ctacaatttt gtgtctcaat ttttttaatt
     9421 tcacctttac tcaaataagt ttttccataa taatgttcta tttttgaata tatcttattt
     9481 gcagtgtctt catcaacata tataactcgg tctgctgaat tgctacattt agaatcatca
     9541 aaatccccta caccttctac aagcatggat gaagatattg aatctatatc actttcaata
     9601 atattttctt cttcatcact ataaagttct tcatacatga aatcagcaaa tgtatcaaaa
     9661 tttacttcta cactttctac attattttca tcaataagac tattttcttc tccatttaat
     9721 atgtattcaa aataagttaa atatataaca tctaatgctt ttaaaatctc cataatatct
     9781 tgattggtat ctatatttct tatatcatta actattcttc tagtcatact atctaatgct
     9841 gggtgcttgt ccatactata aagtacaaat gtgtatttaa ttttatccat aatattatca
     9901 ttgtgtattt tggtaaaacg tgaaacaata tctttataag cattccttct tatgtcttct
     9961 atccctggcc tgtctttaat gaccttttct tcaactattg tatttaatat aacttgaatt
    10021 agacctagta taacatcttt gttgtatcct tttcttattc tatttgtaag atatttttta
    10081 actatatccc aatctacata ctttcttcta gcacctgcaa ttattgcgaa atatagacct
    10141 atttcttttg aagagtaatc tttttctgat atatctatac tttcattgta atcaccagct
    10201 attgtccatg ataggttatt agctctattt tcaaattcat aatcatcata tatccatttc
    10261 ataatctcac ctatattaaa tatgacttat tggaaacaca tctatactct cccatttatt
    10321 aggtattcta gttttaatta catctccaac catttctttc tcatatacat caaaagtctt
    10381 tcctgtaagt cccatattaa ttgcaagagt tggttttaat cctcttctta tagtcttaag
    10441 agatgccata agaccacgta aatcaatagc tttactagat atctctccat tttgagactt
    10501 caattgtaaa tctaaaaata ttcctgcaaa gtgtattaat ttttcttcat cagcatctga
    10561 aaattcattt tttaaaatca tcattaactt atcttcttct ataggaggaa tatctattac
    10621 cataaatcta gataccagtg cttcattaag ttcttttgtt cctgcatatt cataattcat
    10681 agtcccaata aatcttgttg ctggatgtag attgactctc tcatacccag gtacatcaat
    10741 tattcttcta tagtctaaag ctgaatgtac cacaactata gcatcatttt ttgccatatt
    10801 aatttcatca aacaccccaa aacctccatt aatagcacac tcataaactg agcctcttct
    10861 aagtttaact tcattgtcta taaatgtatc tgtacctata agtgttgaac tgtctgtatt
    10921 tatatgaaat gatgtattcc actgaggtct attaaatatt tcccctagat tatcagcaag
    10981 taagtttttt cctgttgcct ttggtcccga taataatata ttttcttctt ctaaaatagc
    11041 tgctatacac atagacaaga tatctttacc ataaaaataa gttttagact ttgttactct
    11101 gtattctaaa tctttttcta aaccataata atttctaaaa tacagtacat cttcaataag
    11161 tttttcatct accccttgca cccttagatt atctattata tccatatttt tacccctttt
    11221 atataaatag tatttgtttc atttcttatc accattttag cataaaagga tataagctaa
    11281 aattgatttt ttaatctccc cattttaagt aaaaccatgc accattttat cactacaaaa
    11341 ttaagagtac tacttactga tatttcttga ttttgttcgt ctatacgtat ataatatatg
    11401 ctgataaatt atgaggaaag gatgattgaa atatggatgc ccagaagtag caaaaaaatg
    11461 gggtatttct gaaagaagag tacaaaaact atgtgaagaa agtcgcatac caggagtttc
    11521 aaaactcggg tatatgtggc tgataccaaa gaacgcagaa aagcctgttg atggtagaac
    11581 aaaaagaaga aaggaagtac aatatgaata gcgttttgat tatagatgat gacaaagaac
    11641 tttgtgctct tatgaaaaaa tgtgttgagc aagaaaattt atctgctatt actgcacatt
    11701 gtggtgcaga aggtctacat ttggtcgata aaaatagaca cacactctct cttgtaattt
    11761 tggacataat gatgccagat atagacggtt ttcaagttct gaaaaaaatt agagaaacaa
    11821 gtaatattcc agtaattatg cttactgcta aaaatgacga ggatgataaa gtttccggac
    11881 tacgtttagg tgctgacgat tacctaacaa aaccgttcag cataaacgaa cttatggctc
    11941 gtgtcaattc tcttattcgt cggtatacta tgttaaaccc tgcttttata acagacacag
    12001 attgcataat aataaatggc atgattattg acaagacaaa ccgaattgtt tctgtcaata
    12061 atattcctgt tcaacttaca agcaaagaat ttgatttact tgcttttctg gcttctaata
    12121 agggacgggt gtttaccaaa aaacagattt atacacaagt atgggaagaa gaatatgctt
    12181 ttgacgatag taacatcatg tctttcatca gtaaattgag aaaaaagatt gaaccagacc
    12241 ccaaccatcc attttatatt ttgacagttc gtggtgtcgg ttatcgtttt aataaggagg
    12301 cttaacatga atacaagtgt ctatctgctt ttgatattgg ttcttagcct gattattata
    12361 gcgtaccttg tttctgtcct gctacgtgtc cgaatacaac ttacacttat aaaagatgct
    12421 ttagaggata taaaaaatgg aaaccttaat cgacgtattt taacagatga aaacgacatt
    12481 acaaaacaaa tttgctatga tattaacgaa atagcaatca atagccaatt tcaacttatt
    12541 cagcaaaaac aatcagaaca agcctacaaa cagctaatga caagtctctc ccatgatgta
    12601 aaaactccac ttgcttcttt ggtcggttat ttggaagcag tagaaagtaa aattgttgta
    12661 ggagaggaaa aagacgagta tatccatgtt gcgtctaata aggctcatta cttaaagaat
    12721 tttgtagaaa atctatttga atgggtaaaa ttggattcta aggaacaagt ttttcatttt
    12781 gatatttttg acttgaatga attatcccgt aatattatat ctgattggat acctgtttta
    12841 gaaagcagtc attttgaata tgaatttgat ataccagaaa tagagtactt cttacgtata
    12901 gacgcaaatg catatacccg tatcattaat aacctattac aaaatattat tacccatagt
    12961 agtggcaaca aaatgacatt gagaattttt gaaaacaaag agcaagctca aattataata
    13021 acagataatg ggaaaggtat atcttcagac aatctcccac atatttttga aaggttgtac
    13081 caatgcgacc attcacgagc ttcaaaggga aatggtttag gcttggcgat tgcaaaggaa
    13141 cttattaacg ctcataaggg aaatattacc gccaacagca ctcccggtat gggaacagaa
    13201 tttacaatat tgcttcctaa agccctgtaa aagtacgagg ctttcttact ctaaaacaag
    13261 ataaaagcaa gattatggca aggttttggc aaggttcatg ttttataatt ctaattgtag
    13321 ttgattttga cgttgtatca actatctttt gtagaaacaa atttattaaa agtgaggaaa
    13381 ttatatatga atgattttgt gattgaaaca aagcaactta caaaaattta tgggaatcaa
    13441 acagtagttg acaaagtcaa tctccatgca aaaaaaggcc gaatatatgg tctgttagga
    13501 cgcaacggag ctgggaaaac cacaattatg aaaatgattt taggattgac ttcaatcacg
    13561 tatggcgagg tagatgtatt tggagagaat attaagggac gtgaaaaaca agtatatccc
    13621 cgtatcggtg caattatcga aacgccagga ttttatccta atctgacagg aacggagaac
    13681 ttggagattt ttgcaaaact gcgtggtaca gccgccccta attctgtaaa aagagcattg
    13741 gaaattgtag gcttacctta taaaaataaa aagctgttta gtgaatattc acttggcatg
    13801 aaacagcgac ttggtattgc caacgctatc ctgcatgacc cagaactgtt agttttagat
    13861 gagccaacca atggtcttga ccctattggt attgcggaga tgagaaagtt tattagggac
    13921 ttgagcgttg agcatggaaa gacaattctt atttccagtc atattctttc agaaatctca
    13981 ttgttggctg atgatattgg aattatcgac cacggtgttc ttttggaaga aagtagtatg
    14041 agtgaactag aaaagaaaaa cagtaaatat atcctgctac aagtttcaga tattccgaag
    14101 acatcactta ttttagagcg tcaatttaac acgaaggatt attccgttca agatgactac
    14161 acgttacacc tttataacac taatctggat atgggtgaaa tcaataaagc acttgtaatg
    14221 cagaatgtaa ctgttattag ttctcaactt tgtaatgata ctttagagga ctactttaaa
    14281 aaaatcacag gaggagaggg cattgcttaa acttattcaa acagaatttc ttaaactgcg
    14341 tcgtaggaaa cttatttggc tgatgttgtt gtctgctctc attatgccgt ttttcgcact
    14401 actatacttc aattattttg gtaaaacagg tgttgagcca ataaagtttt ataaatggtc
    14461 tgcatttggt tatacacttt ggattatttt gcctgttgtt ttgggtatat tttgcaccat
    14521 gcttatacac gaagaaaacc agtatgatat gctcaaacag ctttggattg tacctattag
    14581 taaaatggga tactttttta gtaaattttt cgttgttttg atttactcta tttgttttat
    14641 gctgattaca gccgttgctt ctgtgttatt tagtgtactt tctggatatg tcgtatttag
    14701 ctgggcaagc attttatatt tgttgaaaaa atgtttagaa attggtgtaa tcactgcttt
    14761 tgtaatgttg ccgattttgg caatcgctac atctcaaaaa ggatacattc tcccaatttg
    14821 tatcacattg gtatatgcct tttccggttt tgttataatg acaataaata tgtatttaca
    14881 cccattatcc agtatgggag ttattatcat gagaaataaa gacattccag gtcttatatt
    14941 ttcacaggaa attaatgtcc cattagcatt tctttgtatt tgtatttgga gtattgtatc
    15001 agtattgttt gcaaacactg cactaggaag aagaaagtga ggtgtagata atgtggaaat
    15061 tactttgggc tgaatgtcaa aaaattcgtc gttcaaaaat tgtttggatt acagtttttg
    15121 catccattat gatagcagtc atagtttttg caggagggca ggatgtatat aatgacccag
    15181 atttacatta tggcttaaag agcgtttatg acggttcacg gtatatagac aatgcaggtt
    15241 ggtatatgga cgcagtacaa ccttggtcaa ctttttttgt tcttcctgct gtcattgccc
    15301 tattaggaag ctatattatt tgtcgtgaag aagaggagga cactattaaa tccctgcgtc
    15361 ttattcccat aaatgaaaca aaattaacca tagcaaaaat gattattact tttatattca
    15421 gtatacttct ttatttgtta ctctttacta taacattttt gactgaatcc gttttacatt
    15481 tttccgattt atcgacaaaa ctggttttaa gttgcatgaa agagtatttt cttgatggca
    15541 taggtgtatt ctttgcaatt tctcccatta ttgccttggt atcgcgcatg aaaaaaggat
    15601 attggcttgc attagtattc acagaagttt attctattgc tggactattt gcgggtatgt
    15661 caaatgtctt acaaaccttt tttcctattg cggcaatatt taatatttct ggatactata
    15721 taactactgg agaaaaaatt atggggagta tcataagttt attactttgt gcctgccttt
    15781 ctgcatttat acttaaaggg ctaaaacaca atgaaaaaaa ttgaaatagg tatatcaaaa
    15841 tgaaaaagat aaaaaaactg attattctaa ttgtattctt gtttcttttt ggcatgttac
    15901 actgttatac tgaatggaaa ttcaacaata acccacctaa aggggtaata gaaagaataa
    15961 atgaatggac tgataagatt ggactggatt agtaacttat gttaattata gtgaatgtaa
    16021 tattagcaag ttattgtttc gtattttaat actataactt taaatatttg gttttaatct
    16081 tccaaacgaa tataaaaaaa ctgtcacaga ggcaacatat ttttgaatat ttataaagtg
    16141 gtagttttga ttttcaaagc tgccgccttt tcttctatat aagcaaactt atcctcaaca
    16201 aagtgtaaaa catggtacat tttaagaaaa aagtaccaca gacacaagca ataaaagaaa
    16261 cgccgagaac ctacaaaaac gtagtgctta tgcagattta cgacgccttc tattttctca
    16321 accgtcttaa tgaccttgta caaaagaaat ttttctaata ttttttataa tcttatcttg
    16381 tgatgttttt tatgtataaa tgacttaata acaaatccaa taaaaattat ggatattggt
    16441 ataattagaa cgaggaattt ataatcatta taatgtgaca ttattcttac ccaactctca
    16501 gaaaaaatat atcctatact gattaatgaa gtattccata tggttattcc cacgatagat
    16561 gatattacaa aagttataat attttgacct gctatacctg ctataaaaga tatgtatgtt
    16621 ctacataaag ggattaatct acatacacaa acagaaatag aagagtattt gttaaaataa
    16681 tcttgagatg caaatattcc tttctttgcc tttggacata tctctattat tttatttatg
    16741 atagatttac ctccccaatt tcccactgta taacaaaata tcgaaccaag tatacctgca
    16801 attatactta aaattaaaat tgtcagaaat ccaaaatgat ttcttgcagc aattgctcca
    16861 cataaaggta aaacaacttc gctaggtaat ggaaaacaag catactcaat cataattaat
    16921 acaaatacag agattaatcc atattgtaaa ataaaactat tgattacaga cacttactta
    16981 tctatctcct ttcttattta aaggttttaa gggtacatgt tctaaggatt ctcaccctat
    17041 atttaaagaa tcaatctctt taaactatta tatgtattta cacaaagcat ttataacata
    17101 ctttaaaaat agtaccttaa aataagaaac ttaataaata aaaaaagatt acgtggctac
    17161 gtcctactct cccaggcag
//
LOCUS       DAMJVK010000266        16275 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.guided.093, whole genome shotgun sequence.
ACCESSION   DAMJVK010000266 DAMJVK010000000
VERSION     DAMJVK010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16275
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.guided.093"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            246..863
                     /locus_tag="PC414_003949"
     CDS             246..863
                     /locus_tag="PC414_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDF2466240.1"
                     /translation="MCKKTYLLVVSMLIAGLLTACSGSPTKDSGQAQDNTKKEANASN
                     NALKDEKNNENLMEQDFKVPYTDAINIFKDKYKDADIVDLSLERDLNKFVYTVEGVDD
                     NNEYKMKIDANTKDVLEDKTEKLDSEDLNGVARKEKLDLNDIMTPQQAMEIALKEQNG
                     IVKEWSLDKDLDVTFYKIRIDKDKNEYDIKVDSKKGTVLKVEKED"
     gene            971..3502
                     /locus_tag="PC414_003950"
     CDS             971..3502
                     /locus_tag="PC414_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDF2466241.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDENEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVNEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSNYKVNLFDVIEHKQEDILAKIAVSQGVLFGTPTILGDALKPIWDILISL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLYSY
                     TGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVV
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDGKAQKVVFKDGDSVDC
                     DMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTNVKDIYACGDVAQVGNISLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            3936..4637
                     /locus_tag="PC414_003951"
     CDS             3936..4637
                     /locus_tag="PC414_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanG-Cd-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HDF2466242.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KNLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            4687..5769
                     /locus_tag="PC414_003952"
     CDS             4687..5769
                     /locus_tag="PC414_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2466243.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFINHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIIIFKVIIPIVGNL"
     gene            6030..7130
                     /gene="vanG"
                     /locus_tag="PC414_003953"
     CDS             6030..7130
                     /gene="vanG"
                     /locus_tag="PC414_003953"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HDF2466244.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNDDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            7127..7933
                     /locus_tag="PC414_003954"
     CDS             7127..7933
                     /locus_tag="PC414_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDF2466245.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            7951..10089
                     /gene="vanT"
                     /locus_tag="PC414_003955"
     CDS             7951..10089
                     /gene="vanT"
                     /locus_tag="PC414_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HDF2466246.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKRLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
     gene            10403..11023
                     /locus_tag="PC414_003956"
     CDS             10403..11023
                     /locus_tag="PC414_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDF2466247.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
     gene            complement(11189..11725)
                     /locus_tag="PC414_003957"
     CDS             complement(11189..11725)
                     /locus_tag="PC414_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDF2466248.1"
                     /translation="MIKETHERGGYIFALLILPFINNLYLVKYDIAYKIVLIIIYIYF
                     AYLGSLFPDIDMRGSYISKKFMLIYKLFGSRFRHRGFTHSLIALLLIYSFFKSLTIFT
                     NNNIVFSCLSSGFIIGYLSHLCLDLITKEGIELFYPITINISLLPIKTNSKTEKFISK
                     LLNFIVIFLIGYQFYILL"
     gene            complement(11838..12512)
                     /locus_tag="PC414_003958"
     CDS             complement(11838..12512)
                     /locus_tag="PC414_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HDF2466249.1"
                     /translation="MSLFIISQCITSFAFTPENNKFKVKPLPYAYDALEPYIDKETMK
                     LHHDKHYQAYVDKLNAALEKYPELYNYSLCELLQNLDSLPKDIATTVRNNAGGAYNHK
                     FFFDIMTPEKTIPSESLKEAIDRDFGSFEKFKQEFQKSALDVFGSGWAWLVATKDGKL
                     SIMTTPNQDSPVSKNLTPIIGLDVWEHAYYLKYQNRRNEYIDNWFNVVNWNGALENYK
                     NLKSQD"
     gene            12715..14589
                     /gene="asnB"
                     /locus_tag="PC414_003959"
     CDS             12715..14589
                     /gene="asnB"
                     /locus_tag="PC414_003959"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HDF2466250.1"
                     /translation="MDGNISIYYKGDISKKEDIKFKIKESVNNTRLSSRIEYKDEEKT
                     FDFERFNVIDAIYGNKPFEKDGKVIVFNGNIYNYHEIKEELIKKGHNFTTNDDIEVLL
                     ASYIEFGKNCVHKIKGMFNFIIYDRENESIFGARDLFGIKPLYYINKENAIIFSSEYK
                     FLLEYMKNLNINERSLQSYFSFQYVLPEDTMIQGIRLIPAGHYFRVENGILSLKRYNK
                     LEFRSSTKFFYTKNHLGNRDVNEDDIRNIVVDSIVTHMEEEKEIGTFLSGGIDSSIIT
                     TVASQINPNIKSFSAGFSVKGYSELEVAKKTADKLGIENIQINITQDEYIKSLPNVIY
                     SLDDPIADPSEVGLYFLIKEAGKHVKVALSGEGADELFGGYNIYKEYSTMKSVVNSPT
                     YIKSILGRVSELMPNIKGRNYLYRATTPLEKRYIGNAKVFENSEVKRFFFKYKEKNIY
                     EYLLSNLYRDAQKNNYDYISKMQHIDVNTWLQGDILQKISKLSTAEQVELRVPFLYKD
                     VFDVAKNLRMEQKINKNNTKVLLREAFREIVPEHVVQRKKLGFPTPIRVWLKDSLGDI
                     VKETIYNSNVDEFIDKKYAIKLLDTHLKGHRDNSRKIWTIFTFCLWHQLFIEHKNVEY
                     "
     gene            complement(14681..16117)
                     /locus_tag="PC414_003960"
     CDS             complement(14681..16117)
                     /locus_tag="PC414_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDF2466251.1"
                     /translation="MICTIKPLKISIKKSTEPLIEFISDTTYKNKSSSYIKEVYFTRY
                     IGVDDSIDKYIEKIKNIDTYFFNNPTIPYIRLNNLKINFDKEQTDKMLKIFNIYNSSK
                     FNSWDLYNIDFPCKIENDTLNWTKKIAFKNTLDLFSTSMPNCTSSIIKNFAVKLLCWI
                     DYFLPKLFYNKVKTTISPKIVYFGNIKRQELFFLYFLSQLGCDILYINPNKDILDLYP
                     ECSKFSTLFELSRKTPNILEIPFERHITNINYSENTNKTSPTTNKPNSKNTLNNTNLT
                     SKKIVQDEEVELSYEDLAKLSASVVMIVVCNNENKPFKSGSGVVINNEGYILTNLHVV
                     NDGYSFLVRFENDDKVYTSYQIIKYHSDYDLAVIKVDRKCKPIPVKVSKKPVRGQKIV
                     AIGSPLGLFNTVSDGIISAFRDFETVQMIQFTAPISSGSSGGALLDMFGNLLGLISAG
                     YDDGQNLNLAVESSLVKIFANNFIEIVN"
BASE COUNT         6209 a         1774 c         2723 g         5569 t
ORIGIN      
        1 caaaatgaac tttttagttc atgaggcact ctttttgtac gaaataaaat atatttttat
       61 tatttcaaca atgaaaaaga ggcttgtttc cattttgaga cagtataatt tttatgtaac
      121 tttaatttta gtaaatataa agttttaaaa ttgataaaat aggaggaatt tttaatataa
      181 aagggtaaat aaagggttta gagggcataa tatagaataa attaatatat ttaaggaggg
      241 tttttatgtg taaaaaaacg tatttattag tggtaagtat gcttatagcg ggtttattaa
      301 ctgcatgtag tgggagtcca actaaggatt caggacaagc acaagataat acaaaaaagg
      361 aggcaaatgc atcaaataac gctttaaaag atgaaaaaaa taatgaaaat ttaatggaac
      421 aagattttaa agtgccatac acagatgcaa taaacatatt taaagataaa tataaggatg
      481 ctgatatagt agatttaagt cttgagagag atttaaataa atttgtttat actgtggaag
      541 gtgtagatga taataatgaa tataagatga aaattgatgc taatacaaaa gatgtattag
      601 aagataaaac agaaaagttg gattcagaag atttaaatgg agttgctaga aaagaaaaat
      661 tagatttaaa tgacataatg acacctcaac aagctatgga aattgcatta aaagaacaaa
      721 atggaatagt taaagaatgg agtttagaca aagatttaga tgttactttt tataaaataa
      781 gaatagataa agataaaaat gaatatgaca taaaagtaga ttctaaaaag ggaactgtat
      841 tgaaggtaga aaaagaagat tagaagattt aaactatctt taaaataatt tcttttttga
      901 ataaattttg tttaaaatat aacaagcagg gtataaagag actaaataac tattttagga
      961 ggagtacggt atgtcattga aatcactaaa aataaaagaa aatttatatt gggtaggttc
     1021 gcttgaccct gatttaagag tatttgacat aattatgtat acaccttatg gaactactta
     1081 taattcatat gtattaaaag gaacagaaaa aacagtttta tttgagacag ttaaagataa
     1141 gcattttgac aattatattg aaagattaaa tgatttaaat atagattttg aaaaaataga
     1201 ctatattgtt gtaagtcata ctgaaccaga ccatgcagga agcgttgaaa agcttttgga
     1261 tttagctaaa aatgcaaaag tagtagcttc agaaacagca attaaatatc ttaaggaaat
     1321 cgtaaacaaa gactttgaat atgttgcagt tacagatgga gatacattat caattgggga
     1381 caaaacttta gaatttttct ctgtacctat gcttcattgg ccagatacca tatacacata
     1441 tataaaagag gataaaactc ttgtaacatg tgattcattt ggtagtcact atagtaatga
     1501 taagatagta aatacacttg atgaaaatga agaaaaggat tacttggatg ctttaagata
     1561 ctattatgat tgtataatgg gtccatttaa accatctatg gtaactgcta tagaaaagat
     1621 aaaagattta gatatagata ctgtatgtcc aggtcatggt ccagtattaa ctgaaaatcc
     1681 tagaaaaatt atagaccttt attacaactg gagtgtaaat gaacaaataa agttagaaaa
     1741 agaagttaca atttgttatg tttctgcaca tggatataca aagattatgg cagaagctat
     1801 aaaagcatat attgaaaaaa atagcaacta taaagtaaat ttatttgatg taatagagca
     1861 taagcaagaa gatattttgg caaaaatagc agtatcacaa ggtgtattat ttggaacacc
     1921 aacaatatta ggagatgcgt taaagcctat atgggatata ttaatatcgt taaacccagt
     1981 tcttcatggt ggaaaagttg cttcagtatt tggttcatat ggatggagtg gagaaggtat
     2041 agaaaatgct atggagagaa taagtcagct tagaatgaca gcagtaaaac cttttgctgt
     2101 taactttaaa ccatcaaatg aagagattga taaattatat tcttatacag gtaaattcct
     2161 agataagtta aattctactt ttggaagtaa aaagaaaact aagaaattta aatgtgtaat
     2221 ttgtaatgaa gtttttgaag gtgatagtgc tcctagtgta tgccctgtat gtggagctaa
     2281 ggaagaccaa tttatagaag ttgaagaaga tgaagttact tttagaaaag atactgatga
     2341 atatttcgta atagtaggaa atggagcagc tggtttttat gcagcagatg ctataagaaa
     2401 aagaaataaa acttgtaaga taactatgat ttctaatgaa gatgagttga cttactatag
     2461 acctgcactt tctgatggta taaatgaaga acttggttca gatttctaca tggaagataa
     2521 agattggtat gataaaaata atatagtagt aatattagga acgaatgtag ataaattaga
     2581 tgaagtgaat aaaactatta tagtgaatga tggagctatt aaatttgata agcttgtaat
     2641 agctacagga agtagaaact ttataccacc aattaagggc catgatttag aaaatgtgtt
     2701 tactcttaga aatataaaag atttatatag tgtaaaagaa gcactagaaa aatctaagaa
     2761 agttgtagtg attggtggcg gtttattggg acttgaagct gcatgggaat ttagactaaa
     2821 aggattagaa gtagtagtag ttgaagccat ggatagcata ctatcaaaac aacttgataa
     2881 agaaggtagt aaaatattag aacaatgtgt aagagataca ggaatagatg taagattggg
     2941 tgtagcagtt gatggtattg aaggtgatgg aaaagctcaa aaagttgtat ttaaagatgg
     3001 agatagtgtc gactgtgata tggtagtatt ttcaattggt gtaagagcaa acactcaaat
     3061 ggttcaggat acatctgtaa aaattgatag aggtattgtt gtagataaga cattacagac
     3121 aaatgttaaa gatatatatg cttgtggaga tgttgcacaa gtgggaaata ttagtttagc
     3181 aatatggcca tcttcagtag aaatgggaaa aatagcaggg gcaaatgcaa gtggagataa
     3241 tttgacattt gaaagtgaag tatatccagt ttcattagat gcaatgaatg taaaagtatt
     3301 tagtattggt aatatacaaa attttgataa agaaatttca tcaaaagatg aaggtcaaag
     3361 aatatataaa aaattattta tgaaagatgg ttcattagtt ggagctatac ttataaatga
     3421 tttaagctgt actgttaaat tgataagatt aatatctgaa aaaggtgatt ttgaggatat
     3481 tatgaaggct gatatactat aatttaaata cattaaaata aaaaggagtt gtctcaaaat
     3541 tgattttttt ctaatcaaat tttaaattca actccttttt tgaattttta tataaaataa
     3601 catggaaatt ttagtaatgt ctgtggttac atacataggt ttaattgttt tttatatgga
     3661 ttgtctctgg tataataaat tgaattgtgt ttgactgata gatattttta ttgaaagtta
     3721 atacctgtta tgcgataata aggacgtgtt aggggagttt ttataataac aattaatctc
     3781 agtttttgtg ttccaatagt agcatcaaca cattctgatg tagtagttct tttcttataa
     3841 aaattagtta caaatatgct tgtaattatt tgatatgtaa atttaccaat attataaatg
     3901 atgacctcga taataaggag agtgaaaaat aatctatgaa cgaaaatatt ttgattgtgg
     3961 atgatgaaaa agaaatagca gatttaattg aagtatattt aaaaaatgaa ggttttacag
     4021 tatacaagtt ttatacaggt aaggaagctc tagaatgtat tgaatctaaa aatttggata
     4081 tggctatttt agatattatg cttcctgata ttgatggatt tcatatttgc cagaaaattc
     4141 gagaaaaata ctattatcct atcattatgt taacagctaa aattgaagat atagacaaga
     4201 taatgggttt aaccataggt gctgatgatt atataacaaa gccatttaac cctttggaag
     4261 ttgttgcaag agtaaagaca caattaagaa gatatatgcg atataataat tcttatgaac
     4321 aacagtcgat aattgttaat gagtatgata ttaaaggatt gattatcaat aaagaaacac
     4381 ataaatgtag tttatttggg aaagaagtag cattaacacc gattgaattt tctattcttt
     4441 ggtatttatg tgaacatcaa ggaaaagtgg ttccttctga agaattgttt gaagctgtat
     4501 ggggagaaaa atatcttgat aataataaca cagttatggc tcatataggg cgattacgtg
     4561 aaaaaattca tgaaccttct aaaaacccga aatttataaa aactgtatgg ggagtaggtt
     4621 atacaattga aaagtaaaaa tattaaagtc aatcctgact ttactcaagt gagaaataaa
     4681 atttttatga gaacagcttt aatggcattt atagccatta ttttcatatg gattttatat
     4741 tcttttatat tgtatggtaa gttttctatc tggatagtat cattctttga aaatgctttt
     4801 cgtatggaat ataaagctgc attgaattta tatcagcaga ttttccgtaa tagcatggag
     4861 ctcttcatga tactagctat ggtgattgtg ttttttataa tattccgggt ttatttaaat
     4921 tggtttacaa aatattttag tgaaataaat caaggtattg actctcttat caaagaagat
     4981 gttggagaag tggctttatc tccagagtta ttggcaatag aaaagaaaat aaactcaatt
     5041 aaacatatac ttgaacaacg taaatttgag acacaaatgg cagaacaacg taagaatgaa
     5101 cttattgttt atcttgcaca tgatttaaaa acaccactta catcagttat agggtatctg
     5161 acactacttc gagatgaaag tcagatttct gaagaactgc gaaaaaaata tttatctatt
     5221 tcgttggata aggcagagcg tcttgaagat ttaattaatg aattctttga aattacaagg
     5281 tttaatttat caaatatatc acttgaatac agtacagtaa gcttaactcg tatgcttgaa
     5341 caactaacat atgagtttaa acctactttg atgagtaaga atttaaaata tacactgaat
     5401 gttacaccaa atatgatgat aaggtgtgat gttaataaaa tgcaacgtgt atttgataac
     5461 ttattacgaa atgctgttta ttatagtttt gaagatacta ctattgaaat aacagctgta
     5521 caggaggatg attatataaa gcttaaattt ataaatcata gcaatacgat tccagaggaa
     5581 aaacttgagc gtatttttga acaattttac aggttggata catctcgtaa ttcaaacagt
     5641 ggtagagctg ggttgggact tgctatagct aaagaaatag tggagcttca taacgggaca
     5701 ataacagctc atagcgagaa caatataatt atctttaagg ttattattcc gattgtagga
     5761 aatttgtaag aatttaagta taaaaaaata ggaaattctt taggataaat aaataaagag
     5821 cactcttgtt attgatataa ttactgtatc aaaacaagag tgcttttatt ttgatacagt
     5881 aattatatca atagaaaaat gtgaaaatat attatgtgga acagatgcaa ttgtaagtga
     5941 aggttgattg gatttgaaat agaaaaagta ttttaacatg tttctgtaga ttgtataaaa
     6001 ataaataaat caacaaagga gaatcaaaga tgaataataa aaaaattgca gttatatttg
     6061 gtggaaactc aacagaatat gatgtatcat tacaatcagc ttttgcagta cttgagaatt
     6121 tgaatacgga aaaatatgat attataccaa ttggaattac aagggagggt aattggtatc
     6181 attatattgg agattataat aatatccaga ataacacttg gtttgaaagg gatactgata
     6241 taattccagt tattgtttcg cagaaccgtg tcaaaaatgg tattattgaa tttttaattc
     6301 ctgatgctgt ttctcagaat catattccaa atagtgctat tgaatcatgt gaaaatgata
     6361 ttaaatacac taaacttgat tttgtgtttc ctgtactgca tggaaaaaat ggagaagatg
     6421 gaacagtgca ggggttattt gaattggcag gtatacaggt tataggatgt ggtactcttt
     6481 cttcagcatt atgtatggac aaagacaaat cacataaact tgttcattca gaaggtatta
     6541 aagtaccaaa atcaatatta ctaaaatact cagataaatt taatgtggct gaactgtcaa
     6601 aagacttaac ttatccatta tttataaaac ctgtgcgtgc aggttcatca tttggtatta
     6661 gtaaaattta taaaaaagaa gagttacaag tagcagtaga cttggctttt gaacatgata
     6721 atgaagtaat aattgaggaa aatattgatg gttttgaggt agggtgtgca gttttaggaa
     6781 acgatgactt acttgttgga agagtagatg aaatagaatt gtcaaaagga ttttttgatt
     6841 atacagaaaa atatacattg aaaagttcaa aaattcatat gccagctaga attgatttgg
     6901 attgtgagaa aagaatacaa gagacagcaa aaattattta tagaactctt ggatgttctg
     6961 gatttgctag agtagatatg tttttaactc catctggtga aattattttt aatgaagtca
     7021 atacaattcc tggctttact tctcatagtc gttatccaaa tatgatgaaa ggtattggat
     7081 tgtcttttga agaaatattg gacaagataa ttgaggtgta cataaaatga tgaataatat
     7141 tttattagat aaaaataaag aagataaaat gacaaatact atcttattga ctaaagataa
     7201 agtttatgat gggaatctaa ttctagtaaa tgctttttta ccagttaaaa catctgaaga
     7261 tatagattta attcctgtag atacaaggtt tccaagtatt cttatgaaac gtgaagctac
     7321 aaacatttta caaaatattt taaaaagtat atgtggaata aatgaaatag tacctgtaag
     7381 tggatatcgt actgcagagg aacaacaaga tatatattca agttctctgc gtgacaatgg
     7441 aaaagacttt actaaaaagt ttgtagcact tccaaatcac agtgaacatc agacagggct
     7501 tgctattgat ttagggttaa agaaagaagt tatagatttt atctgccctg aatttcctta
     7561 taatggtatt tgtaatgagt ttcgtaaggc agcactacat tatggtttta tagagcgtta
     7621 tcagtgtaaa aaagaagaaa taacaggaat atctcaagaa ccatggcatt ttcgttatgt
     7681 gggatatccg cattctgaga ttatcaataa aatgaatctc gctcttgaag aatatataga
     7741 atatattaag agattttcgt atacaaattg tttaaaaata gaagataaag aaaaaattat
     7801 agaaatcttt tatgtgccta ttcatactaa agaagaagtg agaatctcta tacctgaaca
     7861 atgtgtatat caaatatcgg gtaacaataa ggatggtttt attgtgacat tttggagaaa
     7921 tagaaaggac taagaaatgg aggaacaaga atgattagga ataaatctta tgcagggctt
     7981 gattatttta ggattatagc tgcattactt ataattgcaa ttcatacatc tccattatta
     8041 tcatatagta aaatggctga ctttgtgtta actagtataa ttgctcgtat tgctgtgcca
     8101 ttctttttta tgctatcagg cttttttcta atttcaacat ataattataa ttgtaataaa
     8161 ctgaaatctt ttctaaaaaa tgtattattt atttacggga tagctattgt aatttatatt
     8221 cccttaaata tttacactgg atatttttcc atgaataatt tagtgcccaa tattattaag
     8281 gacattgtat ttgatgggac tttatatcat ttatggtatc tacctgcatc aattattggt
     8341 gcaagtattg catactttct tgtaaaaaga ctaggtttaa agtactcact agtaatcact
     8401 attgttattt atgtaattgg tatgtttggt gatagttatt atggtttttt agaaaaaata
     8461 ccactattaa agaatttata tgggtatatt tttgaagtat cagattatac acgaaatggt
     8521 atattttttg cacctgtatt tttcatactt ggtggaataa tatctaataa atctaattat
     8581 atgtcaacta aaaatagctt gattggattt cttatatcat tcttacttat gttatgtgag
     8641 ggaatgtttt tacataaact aggggtacag cgtcatgata gcatgtatat tatgttattg
     8701 ccatgtatgt atttcttatt tacagcctta acatttttga aaggttctcg agctaggtta
     8761 ttgcgaacat catcattaat tatttatatt atacatccta tgatgattgt tgttattcgt
     8821 atgttttcta aaatattcgg aatacaaact gtcttggttg acaatagcct tattcattat
     8881 atagtggtaa gtatagcatc ttttatagct tctataatta ttatattgat tcttaaacct
     8941 ctaaaaaaag acttgaaagg agaacataag gtaaatacca atagagcttg gattgaaatt
     9001 gatttaaata atttggaaca taatgtaaag atgatacaaa aagctatgcc agaagattgt
     9061 gaattgatgg cagttgtaaa agctaatgcc tatggtcata gtgcttttga agtagcgact
     9121 tgtgcaaacc aaataggtgt aaaagcattt gcagtagcaa caatagatga ggggattgat
     9181 ttacgtcgat atggaatact aggagaaata ttgattcttg gatatactaa cccaataagg
     9241 gcaaaggaac ttcataaata caatcttaca cagagtgtta ttgaccttaa tcatgctatc
     9301 tctttaaaca aacagaaata caacataaaa tcacacatta aaattgatac aggtatgcac
     9361 agactgggtt ttgatgttaa tgatgtgagg tcaattttaa aggcatttaa attaaaatac
     9421 ttagatatat gtggcatatt tacacacttg tgtgtttcag atagtctgaa agatgaagat
     9481 gtagacttta caaataaaca aattgaatgt ttttataagt taattgattt tctgttaaaa
     9541 aaaggtataa aaattcctaa agtacatatt caaagtagtt atggtttttt taattatccc
     9601 aatcttaagt gtaattatgt aagaattgga attgcattat acgggatatt aagttctcca
     9661 aatgatacga ctaaattgca acttgattta cgacctgttt tatccttaaa atcacaagta
     9721 attcttatta gaaaaataca aaaagatgat agttttggat atggaagagt ctttattgca
     9781 agcagagata gcgtaattgc tatattatct attggctatg ccgatggtat acctagaaat
     9841 ttatcctgtg gaaaaagtta cgtaataata aatggttgta gagcagcaat agtgggtcga
     9901 atttgcatgg accaacttgc tgttgatatt acagatatac ctaatgtaga agttggtaat
     9961 acggcaataa taataggaag agataattta tctgaattat ctgcttctga agttgcaaat
    10021 aactcatgta gtatatcgaa tgaattattg agcagagtag gaagaaggtt aaatgttata
    10081 aagaagtaac tcaattaaat agtaatattt gttgtactgt gtaatttatt ttactctgac
    10141 tagacatttt tagtaatatt atgtctaata tatatttttt ataacttaca tgacaattta
    10201 agttataaaa aggtgatgtt tcagattttt attttgaaac atcacctttt ttgttataat
    10261 atataataaa ataattatac cttaactgta aacttatata gattatttac tttttaatat
    10321 tagattcatt ttttgagatt ggtatataat aaacatatag agtatataat aatagttaaa
    10381 gttaaaaaag gggaaaagca atatgcagta tatacaagaa ctacttgatt tgactcaaaa
    10441 ttattgggtg ttagccacaa taatcggctt attaagtgca tttatagaaa gctttatacc
    10501 agccttacca cttgttgcta tagttacagc aaatgctgca atacaaggat tatttattgg
    10561 atgtttgtta tcatggattg gctcaggact tgggacaact tcattattct tattaataag
    10621 cagatttaat gatagtaagc tttttaataa gttgagaaat agtaaaactg aaaaagcaat
    10681 aagttggatg gataaacagg gatttaagtt attatttata gcgtatgcct gtccatttat
    10741 gcccggttgt ttagttacta tcgcatcagc ttttggtaaa aaagatttaa aagattttgt
    10801 gccagcaatg ttggcaggaa aatttgtaat gtttatagtt ataagttacg tggcaagtga
    10861 tatagaaggt tttataacaa gtcctttaaa gatagcatca tttatattat tggtgttttt
    10921 gtcatggaag attggaaata gagtaaataa aaatcttgaa aatcataatt atgatttcca
    10981 tcataaaaaa catcacaatg ataaagatga taaaatgatt tagcttgtac gaaataaaac
    11041 gtatcttaaa atgatttagt actaaattat tttaagatac gttttatttt ttgtattaag
    11101 gtacaaaatt tttagttctt ttgtaaacta aaatatataa aacttattta tacttagttc
    11161 aaaatattgt tatttatttt ttctacaact ataataaaat ataaaattga tatccaatca
    11221 aaaaaataac tatgaaattt aatagcttag atatgaattt ttctgttttt gagttagttt
    11281 ttataggtag taaagaaatg tttatggtaa tgggataaaa caattcaata ccttctttag
    11341 taataaggtc aagacacaag tgagatagat atcctattat aaacccactg gacaaacatg
    11401 agaaaactat attattgtta gtgaagattg tcagagattt aaaaaaacta taaataagta
    11461 ataaggcaat tagactgtga gtaaaccctc tatgtctaaa cctacttcca aataatttgt
    11521 atattaacat aaatttttta cttatgtaag aacctctcat atctatatct ggaaataaag
    11581 aacctaaata tgcaaaataa atataaatta taattaatac tattttatat gcaatatcgt
    11641 atttaactag gtatagattg tttataaagg ggagtatcaa aagtgcgaat atataacctc
    11701 ccctttcatg tgtttctttt atcatataaa aatcctcctg aatcgtcact ataaacatta
    11761 tattggtaaa tttagactat cttttattat attatatagc aaacataagt ttttttcaaa
    11821 aggattttaa tatttaatta atcttgagat tttaaatttt tgtaattttc taaagcacca
    11881 ttccaattta ctacattaaa ccagttgtca atgtattcat ttcttctatt ttgatatttt
    11941 aaatagtaag catgctccca aacatcaagt cctattatag gagttaggtt tttacttaca
    12001 gggctatcct gatttggagt agtcataata gataatttgc catctttagt agctacaagc
    12061 caagcccaac cagaaccaaa gacatctaaa gcagattttt ggaactcttg cttaaatttt
    12121 tcaaaagaac caaagtctct atcaatagct tcttttaaag attcagaagg tatggttttt
    12181 tctggcgtca ttatatcaaa aaagaattta tgattataag ctccacctgc attatttctt
    12241 acagttgtag caatatcttt aggtaaagaa tctaaatttt gcaataattc acataaagaa
    12301 taattataaa gctcaggata tttttcaaga gcagcattta atttatcaac ataagcttga
    12361 taatgcttat catgatgcag tttcattgtt tctttatcta tataaggttc aagtgcatca
    12421 tatgcataag gtaatggttt aaccttaaat ttgttatttt caggtgtaaa agcaaatgaa
    12481 gttatgcact gtgagattat aaataaagac ataataactg gtattaatat ttttttcttc
    12541 ataaaaatca cctcctaata tagtatttct agaaataaaa aaaatatgaa atattaacag
    12601 ataatttata agaatttaat agcaatttca ttttaaattg aataagtttt aaagccaaag
    12661 acaaaactat gaaatataga ataagactaa aggacagaaa ggaattttga ttatatggac
    12721 ggcaatattt caatttatta taaaggtgat atatctaaaa aagaagatat taaatttaaa
    12781 ataaaagaat ctgtaaataa tacaagactt tctagtagaa tagagtataa ggatgaggaa
    12841 aaaacttttg attttgaaag atttaatgta attgatgcta tttatggaaa taaaccattt
    12901 gaaaaagatg gaaaagttat tgtttttaat ggaaatatat ataactatca tgaaataaaa
    12961 gaagaattaa ttaaaaaagg acacaatttt actacaaatg atgatattga agtcttatta
    13021 gcatcttata tagaatttgg taagaattgt gtacataaga ttaaaggaat gttcaacttt
    13081 ataatctatg atagagaaaa tgaaagtata tttggagcaa gagatttatt tgggataaaa
    13141 ccactttatt atataaataa ggaaaatgca ataatttttt cctcagagta taagttttta
    13201 cttgaatata tgaaaaattt aaatataaat gaaagaagct tacaaagtta tttttcattt
    13261 caatatgtac ttcctgaaga tactatgata caaggaataa ggctgatacc agcaggtcac
    13321 tattttaggg tagaaaatgg tatactatct ttaaagagat ataataaact tgagtttaga
    13381 agtagtacaa aattttttta tactaaaaat cacttaggca atagagatgt gaatgaggat
    13441 gatataagaa atatagtagt agattcaata gtaactcata tggaagagga gaaagaaatt
    13501 ggaacatttt tgtctggtgg aatagattca tctattataa ctactgtggc ttcacaaatt
    13561 aatccaaata taaaatcttt ttctgcaggc tttagtgtca aaggatatag tgaattagaa
    13621 gtagcaaaga aaacagctga taagttaggt atagaaaata tccagattaa tataacacaa
    13681 gatgaatata taaaatcatt accgaatgta atttattctt tggatgaccc tatagcagac
    13741 ccttcagaag taggtcttta tttcttaatt aaagaggcag gtaaacatgt taaagtagca
    13801 ttgtctggag aaggagcaga tgaattattt ggaggatata atatatataa agagtatagt
    13861 actatgaaat cagttgtcaa ttcacctact tatattaaaa gtatattagg aagagtttca
    13921 gaacttatgc caaacataaa aggaagaaat tatctttata gagctacaac accattagaa
    13981 aaaagatata taggaaatgc aaaagttttt gaaaacagcg aagtaaaaag attctttttt
    14041 aagtataagg aaaagaatat atatgaatac ctgttgtcta atttgtatag agatgcacaa
    14101 aaaaataatt acgattatat aagtaaaatg caacatatag atgtgaatac atggcttcaa
    14161 ggcgatatac ttcaaaaaat tagcaaactt tctacagctg agcaggttga acttagagta
    14221 ccatttttat ataaagatgt atttgatgtc gcaaaaaatt taagaatgga gcaaaaaata
    14281 aataagaata atacaaaagt tttacttaga gaggcgttta gagaaatagt cccagaacat
    14341 gttgttcaaa ggaaaaaatt aggatttcca actccaataa gagtttggtt aaaagattct
    14401 ctaggggata ttgtaaagga aactatttat aattcaaatg tagatgaatt tatagataaa
    14461 aaatatgcaa taaaactttt agatacgcat cttaaaggtc atagagataa ttcgagaaaa
    14521 atatggacta tatttacatt ttgtctatgg catcaattat ttattgagca taaaaatgtc
    14581 gagtattaat acttttttca atattaatca gagggcaaaa gcatgcaatg ttttcaccct
    14641 cttgattatt atggaatagg ataagtagaa ttttgattga ttagtttact atctcaataa
    14701 aattattggc aaatattttg acaagactac tttcaacagc taaatttaag ttttgtccat
    14761 catcatatcc agcggaaatc aatcctagta agtttccaaa catatctaaa agagctcctc
    14821 ctgaactacc actagagatt ggagctgtaa attgtatcat ttgaaccgtt tcaaaatcac
    14881 gaaaagctga aataattcca tctgaaactg tattaaaaag acctagaggg cttccaattg
    14941 caactatttt ttgacctctc acaggttttt tactgacttt aacaggtatt ggcttgcatt
    15001 ttctatcaac ctttatcaca gctaaatcat aatctgaatg gtattttata atttggtaag
    15061 atgtataaac tttatcatca ttttcaaatc taactaaaaa tgaataccca tcattgacta
    15121 catgaagatt cgtaagtata taaccttcat tatttattac tactccagaa ccgctcttaa
    15181 acggtttatt ttcgttattg cacacaacaa tcataacaac agaagctgat aactttgcaa
    15241 ggtcttcata agaaagttct acttcctcat cttgtacaat ttttttagat gttagatttg
    15301 tgttattaag tgtattttta gagttaggtt tattcgtagt aggagaagtc ttattggtat
    15361 tttcactgta atttatattt gtaatatgcc tttcaaaagg tatttctaat atatttggag
    15421 tcttcctaga aagttcaaat agagtagaaa attttgaaca ttcaggatat aaatcaagaa
    15481 tatctttatt tggatttata tataaaatat cacacccaag ttgtgataaa aaatataaga
    15541 aaaaaagttc ttgcctttta atatttccaa agtatactat ctttggtgaa atagtagtct
    15601 tcaccttgtt ataaaataat ttaggtagaa aatagtctat ccaacacaaa agtttaactg
    15661 caaagttttt gatgatagag gatgtacaat ttggcatact tgtggaaaat aaatctaaag
    15721 tatttttaaa tgcaatcttt ttagtccaat ttaaggtgtc attttctatt ttacatggaa
    15781 agtcgatatt atataaatcc caactgttaa acttagaact attataaata ttaaaaattt
    15841 taagcatctt atctgtttgt tctttgtcaa aatttatttt tagattgttt aatctaatat
    15901 agggaattgt aggattatta aaaaaataag tatctatatt tttaattttt tctatgtatt
    15961 tatcaattga atcatcaaca cctatatatc ttgtaaagta gacttcttta atatatgaag
    16021 aactcttatt tttataggta gtatcagata taaattcaat tagaggttca gtagactttt
    16081 ttattgaaat ctttaatggt ttaatagtac aaatcatatt ttttctccta taataagatg
    16141 atataatcta ttataaaaca acttaaaaca atatagtaca ataattttgt agtttcaaaa
    16201 taaaatatgt acttgtttat ataaatgata tataattaca ataaaattgc cgaaaagccc
    16261 tctcctttag gtgta
//
LOCUS       DAMJVK010000267        15199 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.guided.098, whole genome shotgun sequence.
ACCESSION   DAMJVK010000267 DAMJVK010000000
VERSION     DAMJVK010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15199
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.guided.098"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            278..1231
                     /gene="blaCDD"
                     /locus_tag="PC414_003961"
     CDS             278..1231
                     /gene="blaCDD"
                     /locus_tag="PC414_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HDF2466252.1"
                     /translation="MKRKKNFIWIAILLVGVVMVMYYVGKKHNDINQKTDKNYIKSEL
                     NKSELNKSKIENNNKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETR
                     RSPCSTFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYY
                     KKLIDKVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQ
                     TMAKIFEGDTNFKKEHINILRDIMKIDVNDKNINVYGKTGTGFDEKNKRVDAWFVGML
                     EREGDTYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
     gene            1575..2258
                     /locus_tag="PC414_003962"
     CDS             1575..2258
                     /locus_tag="PC414_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2466253.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHLLGGVDRPTSGKIYVEDTEISSLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
     gene            2255..4915
                     /locus_tag="PC414_003963"
     CDS             2255..4915
                     /locus_tag="PC414_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2466254.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIKLSIEKEGNWHGELFDYTYGSDLPLIENFSSVDDVMVKGQWYVGKINDEKRDYI
                     IYRNATSEYWNSMPEKDTIIEGRRPNNANEIALSKQYFENNPSVKIGDKITLPIGDRI
                     LNGKPLEPIAPKSKGESFKKISEATLTIVGKVDVSTSSVIPAYTALGYLDKTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKRS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYVNSVNYHTDVPEVVFTYGIPAFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTCFLHIIANQEGAKAVYGSDNYKDQRDIAVYLENSEPVE
                     SAFIEKLDNTEGVKSSLYYQKMSASTWLTEKDASKELAKNGGFKNIASSKKYSPIERD
                     GKFRIISNIIGLDDKSFTEYCRQISVDPKLFYDTKNPTSIVYNKEEDINRSTRRNKVY
                     IDYLNLSIGDNIKLNEKVYDEDKGDYTYNMKVGAITSTLPKIVDTSSKYTLMQIVPMS
                     MVQEISKNYDEVSRLRSNRLIGVFKVNNREDIPRVREKLESIYNKDYGSGDYSIEDVL
                     ESEKQKASGIKTMNLIVGFITGLLAMIGLSNVLATVSGNIRSRRQEFAMLRSVGLSPE
                     GIKKMLILEGLFLGITPLLLSIPVQIGIVYAFLRINEIYFIEYLPFAPISTIIGFTIL
                     ILFIVIASYMTGYKQLKNENIVESIKNETI"
     gene            complement(5096..6277)
                     /locus_tag="PC414_003964"
     CDS             complement(5096..6277)
                     /locus_tag="PC414_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2466255.1"
                     /translation="MKTPRNINSTVVFIIFCLIVTFLLSVILTTITSFNEYNVLSKAL
                     GASYEYNPKSTESILKSLKNSSDKDFKTGNEILSKHGYSKSTFWSKNFFYFLVISFGI
                     ISTMATVVYIIKHRTNKQKLSRINNLTQYLEEVNLGKESVLLRCEDEFSHLEDEIYKT
                     VGELRCSKEFALKERQTLSDNLADIAHQLKTPITSMSLMAQLLSENCPDEEIDYINRL
                     TNQISRLERLVSSLLTLSKLDASTLIFESKLIDVHSLLTYAIEPIEGSLREKHQTFTI
                     EASPSVEFEIDINWTLEALLNILKNCSEHIEDGGYIVAYYSQNPLYVEIIIEDNGKGF
                     AQEELPYIFNRFYRGKNASKDSIGIGLALSKSIIERQNGTLHAENCHNGGARFIIKFY
                     T"
     gene            complement(6277..6939)
                     /locus_tag="PC414_003965"
     CDS             complement(6277..6939)
                     /locus_tag="PC414_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2466256.1"
                     /translation="MAKILLLEDNDTITFGIKTSLEKKNHTVCCYNTIAEAKKNFDKT
                     IDLILLDLNLPDGIGYEFCQYVKSIEDTPVIFLTVRDDEKDIIRGLDMGADDYVVKPF
                     LLSVLQSRINAVLRRTHSNTNNTITCENISLNKVEVKAYLDNVEVPLTAGEYKLLLSL
                     MENKNRAMTRTKLLEMLWDVDGNFVNDNTLTVTIKRLREKLKNPPCIKTLRGIGYRME
                     DN"
     gene            complement(7071..7709)
                     /locus_tag="PC414_003966"
     CDS             complement(7071..7709)
                     /locus_tag="PC414_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2466257.1"
                     /translation="MSELSKRDKEKIQRENEIIDKAEKLFCLNGFDNTTMNELAKEVE
                     YTKRTIYKYFSCKEDLFFAVVLRGYKRLWDNVKIESAKGKTGFEKIKLSYFAFHKFYS
                     VEPSLLCLMGMIGIVNSEKSDTDMLFKEKFFSFNKFMFDEIQGMFEIGKNDRSIRHDI
                     EIPTLMYSSIFTLTGFFNLLSVTGKSYLNNFNIDEEKFIETTLALLIDSIKA"
     gene            7888..8493
                     /locus_tag="PC414_003967"
     CDS             7888..8493
                     /locus_tag="PC414_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2466258.1"
                     /translation="MKIKENVLMLDSTRGSNCFIVFDKEIILIDTGLPFMGKNIIKEL
                     KTLGIKLTDIKHILLTHHDVDHISNIKLLKEKTGAKVWAHVEDIPFIIGEKDRPGFKK
                     FIGKAISRRLIKDVEPYGENMQIGNIKIIHTPGHTPGHVCMIFEDVLFAGDLVKNKNG
                     NIVPYPNPWNWDYKKMMKSIRELDNLKYEWICMSHGTPCIK"
     gene            8997..9746
                     /locus_tag="PC414_003968"
     CDS             8997..9746
                     /locus_tag="PC414_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2466259.1"
                     /translation="MRLESLVNQNYDNLNENDKIIIKQILICQREYRNFSCEKIANEC
                     NVSRATLLRLCRKIGLDSFSDLKYLLKNNQLEFVNNDNNFHDVCENYRLLMNELQKIS
                     FQDICKIIYDAETIYIYGTGNEQKSLAQEIKRIFLSVGKCVIDLFDIGEIEFMRESFQ
                     KSDLFIIISLSGETKAGIEIAKFINNHIHTISLTRLDNNTIATLCEYNLYVETQKLDT
                     LQAISYEVVGAFYALLDLLHMNYIEYRRRKE"
     gene            9743..10507
                     /locus_tag="PC414_003969"
     CDS             9743..10507
                     /locus_tag="PC414_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2466260.1"
                     /translation="MKIEGLFNKYYNSFTANDKYICECILNHKEDCIKLSIDEFADKY
                     HISTSALSRFAQKLQLPGYSELRVIIRLNETEVEYKNQSKHQLMDCYNKVINDIEKKD
                     CSIMFERMKKANRIIVFGEGYSQERVAKEMKRIFLPTGKIIYDVYGYDMIQALNHFIK
                     TDDMIFFISLNGDASTIIDFAKEMRMKGIYMVSITKMISNPLARLCDENLYIQSISIS
                     VDKQLDYDVTTPYFILIELLYIKYKMYLESEHIPRK"
     gene            10606..11427
                     /locus_tag="PC414_003970"
     CDS             10606..11427
                     /locus_tag="PC414_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF2466261.1"
                     /translation="MKLYKLLILDIDGTLRDEVYGIPESAKHAIRLCQKNHCSVVICT
                     GRSMGTIQDDVLSLGVDGYIAGGGNYIQYHGELLYNQSFNQRLIKEVVCLLKNREVAF
                     SIESQEKVFMNQKAKEIFETMNQLKGTNSCINKQHIQEKITYENNIEEYKSQDIHKIC
                     LWSNEKVFDEVKDILQDKMELAQRDISSQYYEIIQKDFHKGKAIKRLQERLGVTQKET
                     ICFGDGQNDIVMFQASDVTIAMKNSHQQLKDIATSICEDIFDNGIYKELKRRNII"
     gene            11441..13105
                     /locus_tag="PC414_003971"
     CDS             11441..13105
                     /locus_tag="PC414_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDF2466262.1"
                     /translation="MEKKWWQKEIVYQIYPRSFQDSNNDGIGDLQGIISRLDYLKDLG
                     ITSLWLCPIFKSPMDDNGYDISDYLDIASEFGSMEDLKELIKKSKDRGIKIILDLVLN
                     HTSDEHPWFQEALKNPESPYHDYYIFKKGNDLPNNWRSVFGGSVWEKVENRDEYYFHS
                     FGKKQPDLNWENPILRSELYQMINTWSQMGVAGFRVDAITFIKKNLSFQSLESDGVDG
                     LVKCTKTVRNQPGIEKMLYELKENTFKKYDCMTVAEAAGISYDQLGDFIGEDGFFSMV
                     FDFKHADLDVASGSEWFKRISWTIPDLRKCILDSQMYVQEHGWAANFIENHDQPRATT
                     KYLLQYERNDDAVKMLGAMYFFLRGTPFIYQGQELGMTNFKRDTINDFNDLSSIDQYY
                     RSIDEGYSEEEALEFVNLRSRDNARTPFPWADKENAGFSDTTPWLKVIENYKDINAQS
                     QINNSESILEFYKKMIKLRQDSEYSDCLIYGSIEPIDIESDDIIAYRRQLDLVVIDCY
                     YNFSDKPVEIRVEEKYDIVFQNLDDINLLNKTVRLKPFHAVLLKVR"
     gene            13110..14984
                     /locus_tag="PC414_003972"
     CDS             13110..14984
                     /locus_tag="PC414_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2466263.1"
                     /translation="MNKYNKIANELIKIIGEDNIISITHCATRLRVMVKDREIINDKK
                     VEKVDEVKGVFFTSGQYQIILGTGIVNKVYAEVEKMGLKTLSKKEQDELVKNNETGFK
                     KVMRTLADIFVPIIPVIAATGLFLGLKGCLFNDNVLGLFGMSSANIPLYIQTLVSVLT
                     ETAFAFLPAIIVWSAFKVFGGTPVIGLVIGLMLVSPILPNAYSVADPSNEIEAIMAFG
                     FIPIVGCQGSVLTAIVTAFIGANLEKWFRKHMPNVLDLIFTPFFVMLITMLVILLGVG
                     PIMHTIELKMVDIISLLIDLPLGIGGFIIGFTYPLAVITGLHHTYVMIETSLLANTGF
                     NALITLCAMYGFANIGTCLAFMKKSKNNQVKQTAVGAMLSQLFGISEPVLFGIQLRYN
                     LKPLIIMCASSGLGAAILSILHIQSNSYGLAVLPSYLMYIYDGYNLITYLLASIFVVA
                     FCFIVTCLFGVPKEAINEDEDEELVFNENNENFVSPAKGKIVALENVPDETFSKKMLG
                     DGFAIDIIDGKIVSPISGKLETVVSSGHAFGIKGTNGEVLIHVGIDTVALNGDGFDIA
                     VKQGDMVKQGDVLVNVDLKRIHELGKSTLTMVLFPDGKKVNILDINKDVKIGQRICVE
                     "
BASE COUNT         5709 a         1744 c         2526 g         5220 t
ORIGIN      
        1 attgtaatat ttaaatatta caatagtaag ttttaaatgt ggaatcaatt attatatcac
       61 aaattaaaag gaggacagga tgttattaat agttacttaa ttatcttcgc aataggttta
      121 atcaacataa ttgtactagg tgaagaaaat ataagagata cagctattca tttagtcttg
      181 tttcttataa gtagaaccta gattggagta tattacaaat aatgtctact attttaatag
      241 taggttataa atttaaatat gggagatgat ataaattatg aaaagaaaaa agaattttat
      301 ttggatagct atcttattgg ttggtgtagt tatggtaatg tattatgtag ggaaaaaaca
      361 taacgatata aatcaaaaaa ctgataagaa ttatataaaa tctgaattaa ataagtctga
      421 attaaataaa tccaaaattg aaaacaataa taaaaacaaa aaagaaaaaa ttaatatggt
      481 tgactatagt gattgttttg aaggtattag tggaggagct attttttata atactaaaaa
      541 taaagaatat aatatatata acaaagaatt aattgaaaca agaaggtcac catgttctac
      601 ttttaaaata gtttcaactt taattggttt agaaaaaggg gtaataaact caaaagaatc
      661 agttatggga tatgatggta cagagtatcc aaataaaaat tggaataaaa atcttagttt
      721 agaagaggca tttaaggagt cttgtgtttg gtactacaaa aaattgatag ataaagttga
      781 tgctaaatct gtccaaaaca ttttagatga tttaaagtat gggaattgcg atattagcga
      841 atgggaagga gatttaaaaa atggaaaggg acatttaaat ggattttggt tagaatcttc
      901 attacaaata tctccaaaag aacaagtgca aacaatggca aaaatatttg agggagatac
      961 aaactttaaa aaagagcata ttaatatttt aagagatatt atgaaaatag atgtaaatga
     1021 taaaaacata aatgtatatg gaaaaactgg aacaggattt gatgaaaaaa ataaacgtgt
     1081 ggatgcatgg tttgttggta tgctagagcg tgaaggtgac acctattatt ttgctattaa
     1141 atcagatgat tccaataaag aaattacagg gccaaaggta aaggaaattg caattaatat
     1201 aattaagaaa tattatagtg taagggaata aacttaattt tatttgtaaa tattatttac
     1261 ctatagtctg taatatttat taaataattt tactaaattg agtctatata gagcaaaact
     1321 cctttagtac ataaaatact tgctattgat tattaaattg aatacctata gatttaaagt
     1381 taataccttt taaagactat gtaatatatt aaaagtataa ataaaatata ttgcatagtt
     1441 tttgttttat attataattt taaataatgt gcaaaaacta aacaatattt ttaatagtaa
     1501 tttatatttg tattcatgtc actataatgt cattttaggt tgctaaaatt taaccataaa
     1561 ggagatgatt gtatatggaa atattaaaat gtgaaaatct cacaaaaata tatggttcaa
     1621 atcaaaccag agtgacagcc cttaataatg taaatttatc agttcaaaaa ggtgattttg
     1681 tatccattgt aggagcatct ggttctggca aatctacatt gttacatctg cttgggggag
     1741 tagatagacc tacatctgga aagatttatg ttgaagatac agaaatatca agtttaaagg
     1801 aagaagctct tgcagttttt agacgtagaa aagtaggtct tatttatcaa ttttataatc
     1861 ttatacctac ccttgatgtt agaaaaaata tattattacc catgttactt gataaaagaa
     1921 aggtggatga ggatagattc tctgagattg tatctatatt gggattatct gatagattaa
     1981 accatcttcc aagtcaactt tcaggtggac aacaacaaag ggtatctata gctagaagtt
     2041 taatctatag accagctatt ttattagcag atgagccaac tggtaatctt gatagaaaaa
     2101 attcagaaga aattgtagac ttgttaaact tatcaaataa acgatttaat cagacaatac
     2161 ttcttattac tcatgatgag aaaatagcac tagaggcaaa tcgtattgta actatggaag
     2221 atggagtgat tgtctctgaa aaggtggtga agaaatgaat attcttcaag aatacacctt
     2281 agactttgta cgacgcaata aacgtagtag tatagctata atgttagcta tattacttac
     2341 tacaaccatg atgtcttctt tgtgtggact gttgtatacc atgtggacag attttataaa
     2401 actttcaatt gagaaagaag gtaactggca tggtgaattg tttgactata cttatggaag
     2461 tgacttacca ttaattgaaa atttttcttc agttgatgat gtaatggtaa aaggacaatg
     2521 gtatgtaggc aaaattaatg atgagaaaag agattatata atttatagaa atgctacatc
     2581 agagtattgg aattctatgc cagaaaagga tactataata gagggtcgtc gaccaaataa
     2641 tgctaatgaa attgcattat caaaacaata ttttgagaat aatccaagtg taaaaattgg
     2701 tgataaaatt actctcccaa taggagatag aatcctaaat ggaaagcctc ttgaacccat
     2761 agccccaaaa tcgaaagggg aaagttttaa aaaaatttca gaggctacat tgacaatagt
     2821 aggaaaggta gatgtttcta cttcatcagt aatacctgca tatacagctc ttggatatct
     2881 tgataaaaca agtataaaac ctaatgatag tataactgtg tacttgcgat ttaaaaatat
     2941 aagagacacg tataaggaac ttccaaaact tgctaaatca ttgggatgga agactaatga
     3001 atatggaaaa tacaatctta aatacaatag taattatctt ttaaagatgt tagtcttttc
     3061 accagaacag aaagcatcta tgagtagtat atcaaaattt tcaactccta ttatgtatct
     3121 tacaatagca atttttacag tagccgtgtt tgtaatggtg atttacaacg cgttttcact
     3181 gtctgcaaat gctagactta ctcaattagg gatattatct agtgttggtg catcaccaaa
     3241 acaaattaaa aggtctgtag tgtttgaagg ctttttactg acaattatac cattaccaat
     3301 aggtttattt ttaggatggt tattgtgtaa taggcttata gtttatgtta attcagttaa
     3361 ttatcatact gatgttccag aagtggtatt tacttatggt attcctgctt ttctgccagc
     3421 agttttactt acaatagtaa cagtctggat ttctgcactt ataccagcac gaaaagtatc
     3481 aaagatatct cctatagaag caatacgaca aggagatagt gttaaaatca aaaagtcgca
     3541 taaatattca ttaggaaaga tatttggaat agaaggagaa cttgcatcaa acgcactacg
     3601 tgctcgtaaa aaatcttatc gtactgcaac aatatcttta acattatctt ttttattact
     3661 tacatgtttt ttgcatataa ttgcaaatca agaaggagca aaagctgttt atggaagtga
     3721 taattataag gaccaaagag atatagcagt ttatttggaa aatagtgaac ctgtagagtc
     3781 tgctttcatt gaaaaattag ataatacaga aggtgtaaaa agttcactat attatcaaaa
     3841 aatgtctgct tccacttggt tgactgaaaa agatgcttca aaagaacttg cgaaaaatgg
     3901 aggatttaaa aatatagctt catctaaaaa gtactctcct atagaacgtg atggcaagtt
     3961 tcgtataatt tcaaatataa ttgggcttga tgataagagt tttacagaat attgtaggca
     4021 aatttctgta gacccaaagt tattttatga tacaaaaaat ccaacctcaa ttgtatataa
     4081 taaggaggaa gatattaatc gtagtacacg tagaaataaa gtatatatag attatttaaa
     4141 tctttcaata ggagataaca ttaaattaaa tgaaaaagta tatgacgaag ataaagggga
     4201 ttatacttac aacatgaaag tgggagctat tacaagcaca cttcctaaaa tagtagatac
     4261 tagttcaaag tatacattaa tgcaaatagt tccaatgtct atggtgcagg aaatatctaa
     4321 aaattatgat gaagtaagta ggttacgttc aaataggctg ataggagtat ttaaagtaaa
     4381 taatcgtgag gacattccta gagtaagaga aaaattagag tccatttata ataaagacta
     4441 tggttcagga gattatagta ttgaggatgt tttagaaagt gaaaaacaaa aggctagtgg
     4501 aataaagacc atgaatttaa tagtaggctt tattacaggt ttattggcta tgattggatt
     4561 atcaaatgta cttgctacag tgtcaggaaa tattagaagt agacgacaag agtttgctat
     4621 gttgcgttct gttggattat ctccagaagg cataaaaaag atgcttattt tggaaggttt
     4681 attcttagga attacaccat tactacttag tattcctgtg caaataggaa tagtgtatgc
     4741 ttttctacgt ataaatgaaa tatattttat agagtattta ccatttgcac ctatatctac
     4801 aataataggt tttactatat tgatactttt tattgtaata gcatcatata tgacaggata
     4861 caagcagttg aagaatgaaa atatagtaga atctataaaa aatgaaacaa tataaacaaa
     4921 atctatttga acttatttaa aggtttcgat ttaagactac ttacaagtat agggatagtt
     4981 aagttgtttg tttaataaaa ccttaaatgt aaatagaagt atattactaa aacaatagat
     5041 aacatagaat atagatatag ctggattata ggtattttac ctataatcca gcattttatg
     5101 tgtaaaattt aattatgaat cttgcaccac cattatggca attttctgca tgtagagtac
     5161 cattttgacg ttctataata gactttgata gagcaagccc tataccaata ctgtccttac
     5221 tagcattttt accacgataa aatctattaa aaatatatgg aagttcttcc tgagcaaacc
     5281 ccttaccatt atcttctatg ataatctcaa catatagagg attttgacta taataagcaa
     5341 ctatatatcc accatcttct atatgctcac tacaattttt aagaatattt aaaagagctt
     5401 ctaaagtcca attaatatcg atttcaaatt ctaccgaagg agaagcctcg atagtaaatg
     5461 tctgatgttt ttctcttaaa gaaccttcta taggctcaat tgcataagta agaagagaat
     5521 gtacatctat aagtttgctt tcaaatatta aagtacttgc atccaatttt gataatgtaa
     5581 gtaaagatga aactagtctc tcaagtcttg agatttgatt tgtaagtctg ttaatataat
     5641 ctatttcttc atctggacaa ttttctgaaa gtaattgtgc cataagtgac atagaggtaa
     5701 ttggagtctt aagttgatga gcaatatctg caaggttatc tgaaagagtt tgtctttctt
     5761 ttaaagcaaa ttcttttgaa caacgaagtt ccccaactgt tttatatatc tcatcttcaa
     5821 ggtgagaaaa ttcatcttca catcgtaaaa gtacagactc ttttcctaaa ttcacttctt
     5881 ctaagtattg agtaagattg tttattctac tcagtttttg cttatttgtt ctatgtttga
     5941 ttatatatac aactgtagcc attgttgaaa ttataccaaa cgatatcact agaaaataaa
     6001 aaaagttttt actccaaaat gtacttttag aatatccatg ttttgataaa atttcatttc
     6061 ctgttttaaa atccttatca ctagaatttt tcagtgattt tagtatactt tctgtagact
     6121 taggattgta ctcatatgat gcaccaagag ccttagacag tacattgtat tcattaaaag
     6181 aagttatagt tgttagaatt acactcaata aaaaagttac aattaagcag aatattataa
     6241 aaacaactgt actgttgata tttcttggag ttttcactag ttgtcctcca ttctatatcc
     6301 tatgcctctt aatgttttta tacaaggtgg attttttaat ttttctctaa gacgttttat
     6361 tgtaacagta agagtgttat catttacgaa attaccatct acatcccaga gcatttctag
     6421 taactttgta cgagtcatag ctctattttt attttccata agacttagaa gaagtttata
     6481 ttctcctgct gtcaaaggta cttcaacatt atctaaatat gctttgactt caaccttatt
     6541 caagcttata ttttcacatg taatagtatt gttagtattt gaatgagttc ttctaagaac
     6601 tgcattaatt ctggattgaa gtactgagag caaaaacggt ttaaccacat agtcatctgc
     6661 acccatatct aatccacgta taatgtcttt ttcatcatca cggactgtca aaaatatcac
     6721 tggagtatct tctatacttt taacatattg gcaaaactcg tatccaatac catcaggtag
     6781 atttaaatca agcagaatta aatcaatagt tttatcaaaa ttttttttag cttcagcaat
     6841 cgtattataa caacatacag tgtgattttt cttttcaagg gaagttttta ttccaaatgt
     6901 aattgtatca ttatcttcta aaagaagtat tttagccatt tacttttaat cctttcagta
     6961 atttagttat tccattaact aagttattag ttttatgttt aatatttttc atattagaga
     7021 tagaaaaaat tatcatttaa gtatataaac tttaatttat aaacttaggt ttatgcttta
     7081 attgaatcta ttaaaagagc aagagttgtt tcaataaatt tttcctcatc tatattaaag
     7141 ttgttcaggt atgattttcc tgttacagat agtaaattaa aaaatcctgt aagtgtaaat
     7201 attgatgaat acataagagt aggtatttca atatcatgcc taatacttct atcatttttt
     7261 ccaatttcaa acattccttg tatttcatca aacataaatt tattaaaaga aaaaaacttt
     7321 tctttaaaca gcatatcagt atccgatttt tcagagttta caataccaat cattcccata
     7381 aggcataata gagaaggttc tacagagtaa aatttatgaa aagcaaagta tgaaagtttt
     7441 attttttcaa accctgtttt gccttttgca ctttctattt taacattatc ccaaagtctt
     7501 ttatatcctc ttaagacaac agcaaagaat aaatcttctt tacatgaaaa gtacttgtat
     7561 atggtgcgtt tagtatattc aacttctttt gctaattcat tcatagttgt attatcaaat
     7621 ccattaagac aaaataattt ttctgcctta tcaataatct cattttctct ttgtattttt
     7681 tctttatctc tttttgataa ttctgacata taaattattc ctttctattg ataaaaaagt
     7741 atactgatgg tatatttatg taaatataat atttgtatac ctatagtata ctttaataaa
     7801 aaataaaatt aatatcaagt attataacta tttataactt tattaattaa ttatataggt
     7861 aaaataccaa ataaggggaa tttatttatg aagataaaag aaaatgtatt aatgttagat
     7921 agtactcgtg gctcaaattg ttttattgtg tttgataaag aaattattct tatagacaca
     7981 ggtcttcctt ttatggggaa aaatataatt aaagaattaa aaacacttgg cattaaactt
     8041 acagatatta agcatatttt attaactcat catgatgttg accatatatc taatattaaa
     8101 ttattgaaag aaaaaacagg agcaaaagta tgggctcatg tagaggatat accttttatt
     8161 ataggagaga aggatagacc tgggtttaag aagtttattg gaaaggcaat ttccagaagg
     8221 ctcattaagg atgttgaacc ttatggagag aatatgcaga ttgggaatat aaaaattatc
     8281 catactcctg gtcatactcc agggcatgtc tgcatgattt ttgaagatgt attatttgca
     8341 ggtgatttag tcaaaaataa gaatggaaat atagttcctt atcctaatcc atggaattgg
     8401 gattataaaa agatgatgaa atctataaga gaattggata atttaaaata tgaatggata
     8461 tgtatgtctc atggaactcc ttgtattaaa taataaaaat taacatggat taatagaggc
     8521 gtagttgctt gtatgtatga taataattta cagcttcttt ttaatatatc atgtatattt
     8581 gtagctgtaa gcatatttat aacaatactt acaattttgt attttaacat ctataataat
     8641 aattttagct ttgttaacta tgaaatttac ttaaagaagt aaatattcaa gtagtagtaa
     8701 ttattaaata agataaaaaa taaatggcag atgaattaaa gtaatttcta ccatggaaaa
     8761 tacttttgag ttagaaaaaa ctctatttta gagtatgaag agatgaagct tccgaagaaa
     8821 gcaatatcta aagtacatgt tcgaaaggat aagtactttt tggtgcctgc ttttagcagg
     8881 ttatttttat tttaataaaa ataaaatcaa atgattattt tatccttatt atttaaaact
     8941 cttccctaga aaaagcaact aatgtatatt aaaataatat ttagggagtg gaaaaaatga
     9001 gattagaatc gttagttaac caaaattatg ataatttaaa tgaaaatgat aaaattatta
     9061 ttaaacaaat attgatttgt caaagagaat accgtaattt ttcgtgtgaa aaaattgcaa
     9121 atgaatgtaa tgtttctaga gctactttat taaggctttg tagaaaaatt ggattagatt
     9181 cattctctga tttaaagtat ttacttaaaa ataatcaact agaatttgtt aataatgata
     9241 ataattttca tgatgtttgc gagaattatc gactattaat gaatgaatta caaaaaatat
     9301 catttcaaga tatttgtaaa ataatatatg atgcagaaac aatttatatt tatgggacag
     9361 gtaatgaaca gaaaagttta gctcaagaaa taaagagaat atttctttca gtaggtaaat
     9421 gtgttattga cttatttgat ataggtgaaa ttgaatttat gagagaatca tttcaaaaaa
     9481 gtgatttatt tattatcatt tctttgtctg gagaaacaaa agcaggaatt gaaattgcaa
     9541 aatttatcaa caatcatata catacgattt ctttaactag gcttgataat aatacaattg
     9601 caacactttg tgagtataat ctgtatgttg aaactcaaaa acttgataca ttacaagcta
     9661 tatcttatga agtcgtagga gcattttatg ctctattaga tttgcttcat atgaattaca
     9721 tagagtatag aaggagaaaa gaatgaaaat tgaaggactt ttcaataaat attataattc
     9781 atttacagca aatgataagt atatctgtga gtgtattctt aatcataaag aagattgtat
     9841 taaactttct atagatgaat ttgcagacaa ataccatata tctacaagtg cactttctcg
     9901 ttttgctcaa aaactacaat tacctggata tagtgaatta agagttatta tacgcttaaa
     9961 tgaaacagaa gtagaatata aaaatcaaag taaacaccaa ttaatggatt gctataataa
    10021 ggtgataaat gatatagaga agaaggattg ttcgattatg tttgaaagaa tgaaaaaggc
    10081 aaatcgtata atcgtctttg gagaaggtta ttctcaggaa agagttgcta aagagatgaa
    10141 aaggattttc ttaccaacag ggaaaattat atatgatgtt tatggttatg atatgattca
    10201 ggcacttaat cattttataa aaacagatga tatgattttc tttatttcat tgaatggaga
    10261 tgcatctaca attattgatt ttgctaaaga aatgagaatg aaaggaattt atatggtttc
    10321 tattacaaaa atgataagta atcctttagc aagattatgt gatgaaaacc tgtatattca
    10381 atctatatct atttctgtag ataaacaact ggactacgat gtaacaacac cttattttat
    10441 tttaattgaa ctgctttata ttaaatacaa gatgtattta gaaagtgaac atattccccg
    10501 aaaatgaaaa tttgtgctaa aaatgaacat attttttaaa agatatcaaa atattgatat
    10561 cttttttttt gaatgctatt atgattttga tagtaagaaa ggaagatgaa attgtacaaa
    10621 ttattaatac ttgatataga tggtacgctt agagatgagg tatatggaat cccagaatct
    10681 gcaaagcatg ctatacgttt atgtcaaaag aatcattgca gtgttgttat ttgtactgga
    10741 agaagtatgg gtacaataca agatgatgtt ttatcattag gagttgatgg atatattgca
    10801 ggaggaggta attacattca atatcatggt gaattacttt ataatcaatc ttttaatcaa
    10861 agattgataa aggaagttgt ttgtttatta aaaaatagag aagttgcttt ttcgattgag
    10921 agtcaggaaa aagttttcat gaatcaaaaa gccaaagaaa tatttgaaac aatgaatcag
    10981 ttgaaaggaa ctaattcttg tataaataaa caacatatcc aagaaaaaat aacatatgaa
    11041 aataacattg aagagtataa gtcacaagat atacataaaa tttgtttatg gtcaaatgaa
    11101 aaggtatttg acgaagtgaa agatatacta caagataaaa tggaattagc acagagagat
    11161 atttctagtc agtattatga aattattcaa aaagattttc ataagggcaa agcaattaag
    11221 agattacaag aaagattagg agtaactcaa aaagaaacca tatgttttgg tgatggtcaa
    11281 aatgacattg tcatgtttca agcatcagac gttacaattg caatgaaaaa tagtcatcaa
    11341 caacttaaag atatagcgac atctatatgt gaggacatat tcgataatgg aatttataaa
    11401 gaattaaaaa gaagaaatat catataaaaa ggagtaattt atggaaaaga aatggtggca
    11461 aaaagaaatc gtttatcaaa tttatccaag aagctttcaa gatagtaata atgatggaat
    11521 tggagattta cagggaatta tttctcgttt agattattta aaagatttag gtattacatc
    11581 attgtggtta tgtccaattt ttaaatcacc tatggatgat aatgggtatg atattagtga
    11641 ttatttggat attgcaagtg aatttggaag tatggaagat ttaaaagagt taattaaaaa
    11701 aagtaaagat agaggaatca aaattatatt agatttagtt cttaatcata caagtgatga
    11761 acatccttgg tttcaagaag cattaaaaaa tccagaaagt ccttatcatg attattatat
    11821 ttttaaaaaa ggaaacgatt tgccaaataa ctggaggtct gtttttggtg gaagtgtttg
    11881 ggaaaaggta gagaatagag acgaatatta ttttcattct tttggaaaaa aacaacctga
    11941 ccttaattgg gaaaatccaa tattaagaag tgaactttac caaatgatta atacctggtc
    12001 tcaaatggga gtggcaggat ttagagttga tgctattaca ttcatcaaaa agaatttaag
    12061 ttttcaaagt ttagaaagtg atggtgttga tggtcttgtt aaatgtacaa aaacagttag
    12121 aaatcagcct ggaatagaaa aaatgcttta tgaattaaaa gaaaatacgt ttaaaaaata
    12181 tgattgtatg actgttgctg aagcagcagg tattagctat gaccagttag gtgattttat
    12241 tggagaagat ggtttctttt caatggtctt tgactttaaa catgctgact tagatgttgc
    12301 ttctggtagt gagtggttta aaagaatttc atggacaatt ccagatttaa gaaaatgcat
    12361 tttagatagt caaatgtatg ttcaggaaca tggatgggca gctaatttta ttgaaaatca
    12421 tgaccaacca agagctacaa caaagtatct tttgcaatac gaaaggaatg atgatgcagt
    12481 aaaaatgttg ggagcaatgt atttcttttt aagaggaacc ccatttattt atcagggaca
    12541 ggaattagga atgacaaatt ttaaaagaga taccataaat gattttaatg atttatctag
    12601 cattgaccaa tactatcgtt caatagatga aggttactca gaagaagaag cgttagaatt
    12661 tgtgaattta agaagccgag ataatgcaag aacgccattt ccatgggcag ataaagaaaa
    12721 tgcaggtttt tcagatacaa caccatggtt aaaggttatt gaaaattata aagatattaa
    12781 tgcacagagt cagattaaca attcagagag tattttagaa ttttataaaa agatgataaa
    12841 gttgagacaa gatagtgagt attctgattg tttgatttat ggttcaatag aacctattga
    12901 tatagaaagt gatgatatta ttgcgtatag aaggcaatta gatttggtag ttattgattg
    12961 ttattataat ttttctgata aacctgtaga aataagagta gaagaaaaat atgatattgt
    13021 attccaaaat ttagatgata taaatctatt aaataaaaca gtgcgtttga aaccatttca
    13081 tgcagtatta ttgaaagtga ggtagaaaaa tgaataaata taataaaata gcaaatgagc
    13141 ttattaaaat tattggtgaa gataatatta tttctattac acattgtgcc acaagattaa
    13201 gagtgatggt taaagataga gaaattatta atgataagaa agttgaaaaa gttgatgagg
    13261 ttaaaggggt tttctttact tctggacagt atcaaattat tttaggaaca ggaatagtta
    13321 ataaagttta tgcagaagtt gaaaaaatgg gattaaaaac tttatcaaaa aaagaacaag
    13381 atgaattagt taaaaataat gaaactggat ttaagaaagt tatgagaact ctagcagata
    13441 tatttgttcc tattattcca gttattgcag caacaggatt attcttggga ttaaagggtt
    13501 gtttgtttaa tgataatgta cttggattat ttggaatgtc ttctgcaaat attccattgt
    13561 atattcagac acttgtttcg gttcttacag aaacagcatt tgctttttta ccagcaatta
    13621 ttgtatggtc agcatttaaa gtatttggag gaacacctgt tattggactt gttattggac
    13681 ttatgttagt ttccccaata cttcctaatg catattctgt tgctgaccca tcaaatgaaa
    13741 ttgaagcaat tatggcattt ggatttattc ctattgtagg ttgtcaagga agtgttttaa
    13801 cagctattgt tacagcattt attggagcta atcttgaaaa atggttccgt aaacatatgc
    13861 ctaatgtatt agaccttatt ttcacaccat tttttgttat gctaataaca atgttagtta
    13921 ttttgttagg tgtaggccct ataatgcata caatagaatt aaaaatggtt gatatcatca
    13981 gcttattgat tgatttgcct ttaggaattg gaggatttat tattggattt acatatccat
    14041 tagctgttat tactggctta catcatactt atgtaatgat tgaaacatca ctattagcaa
    14101 atacaggatt taatgccctt attactcttt gtgcaatgta tggatttgcg aatattggaa
    14161 catgtctagc atttatgaaa aaatctaaaa ataatcaagt taaacaaact gcagttggag
    14221 caatgctatc tcaacttttt ggtataagtg agcctgtttt atttgggatt cagcttcgat
    14281 ataatttaaa gcctttgatt attatgtgtg catcttcagg tttaggagca gcaatattat
    14341 caattttaca tattcaatct aattcatatg gattagcagt tttaccttct tatttaatgt
    14401 atatctatga tggatataat ttaatcacat acttattagc atcaatattt gtggttgcat
    14461 tctgctttat tgtaacatgt ttatttggag tgcctaaaga agctataaat gaagatgaag
    14521 acgaagaatt agtgtttaat gaaaataatg aaaactttgt gtctccagct aaggggaaaa
    14581 ttgttgcatt agaaaatgta ccagatgaaa cattttctaa aaaaatgtta ggtgatggtt
    14641 ttgctattga tattatagat ggaaaaattg tttctcctat ttcaggtaaa ttagaaacag
    14701 tggtttcaag tggacatgca tttgggatta aaggaactaa tggagaagta ttaattcatg
    14761 ttggaattga tacagttgca ttaaatggag atggctttga tattgctgtt aaacaaggag
    14821 atatggttaa acagggagat gttcttgtta atgttgattt aaagagaata catgaattag
    14881 gaaagtctac tttaacaatg gtattgtttc cagatggtaa gaaggtaaat attttggata
    14941 taaacaagga tgtaaaaata ggtcaaagaa tatgtgttga gtagtaggtg atatgtaatg
    15001 actacaaaag aagattatca agagtaattc aatcttttta aagtatgaga aataattcat
    15061 aaactctaaa acaattaaag aggttgtttc aaaatagaga ttgtattgca cccaaattgt
    15121 tgaaatgatg aaaatatatt taacttcata caaaaaatta aatagtggca acaattgagg
    15181 atgtgaggga gagttgcca
//
LOCUS       DAMJVK010000268        11979 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH650 isolate JH273
            SAMN32366126-rid16590003.guided.111, whole genome shotgun sequence.
ACCESSION   DAMJVK010000268 DAMJVK010000000
VERSION     DAMJVK010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366126
            Sequence Read Archive: SRR22863035
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560891.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560891.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:05:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,984
            CDSs (total)                      :: 3,901
            Genes (coding)                    :: 3,852
            CDSs (with protein)               :: 3,852
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 23 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 10 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11979
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366126-rid16590003.guided.111"
                     /strain="SH650"
                     /isolate="JH273"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            158..1564
                     /locus_tag="PC414_003973"
     CDS             158..1564
                     /locus_tag="PC414_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDF2466264.1"
                     /translation="MNTGFPKDFLWGASSSAFQVEGAWDKDNKGKTVADYNSFKKSHL
                     QADTKVASDFYHNYEEDIELMKELGMKTYRFSISWARIIPDGEGEINQKGLEFYNKII
                     DKLIECDIEPFVTLYHFDLPFKLVEKYNGWESRETVYAFERFAKICFKHFGDRVKYWQ
                     PHNEQNLIVRVEERINIYDETDSWKIDKMRAQMDYNLCLAHALAVNACHEMIKESKIG
                     AAVSSSVTYPLTSKPEDVYAARMNDNFKVYYMLDMHHYGEYPGYYMKYLEKRNIVPHM
                     EDGDKEILKKAKMDFIAVNYYRTNCAEALPEDSQHPFGLREGTVDFSMYGLFKMSMNP
                     NLEASEYGAAIDPSGLRVALNEYWQRYHLPVIITENGLGAKDILEDGKIHDDYRIDYL
                     RSHINACKLAIEDGVEMIGYCPWSFTDLLSSSQGFNKRYGLVYINRTDHEVLDLKRIK
                     KDSFYWYKEVIENNGIVK"
     gene            1592..2440
                     /locus_tag="PC414_003974"
     CDS             1592..2440
                     /locus_tag="PC414_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2466265.1"
                     /translation="MYITKVLNNSLLLAKDDNDEEIILMGKGIGHNYKAGSELNKKDI
                     EKIFVLHDENIKKSIIQLATEIDAEYFGIAQMIIAYGIEKYNLKLMNHIYLALTDHIA
                     FAVRRFKEGIMIENHYLFEIKEFNPKEYDIGKYAITVFKEVLGLDLPEEEIGNIAGHF
                     INAQQDNPYSDRNKRSAKIVNAILQIVHYHFSIVYNKESFYYRRFVMHLKAFSQRFLA
                     NEPSREKIDFIYEQVQKNCKEEYECVKKVGAYMYKEYSRELPRQEELYLMIHIHKILG
                     ELCENS"
     gene            3101..3793
                     /locus_tag="PC414_003975"
     CDS             3101..3793
                     /locus_tag="PC414_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003020373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2466266.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEQIEANYCHSGRDGLVELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKSDNYSKVHGLRAGADDYLTKP
                     FEITEFTARVLSLIRRYTRFNPKNEEPQQLSFKGLLIDLDQHTVTTNGEPMLLPPKEF
                     GVLLYCARHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            3790..4725
                     /locus_tag="PC414_003976"
     CDS             3790..4725
                     /locus_tag="PC414_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2466267.1"
                     /translation="MKMDEQTIILFLLLLSIGLLFLSIFAFSKLGHIYKRLKDIEEIL
                     ADVQIGDENRKILIKPCDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYLDAVHSGIVIEQEREEYLEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLNFEIDIPENRLMVNLDPDGYSRVVNNLVQN
                     VLAHSKARQIKITMSEDSRMVLLRVEDDGVGIARENLPHIFERLYKCDKGRSEKGSGL
                     GLSIVSQMVERMGGQVSVESEIGKYTVFTVSLPLI"
     gene            4873..5796
                     /locus_tag="PC414_003977"
     CDS             4873..5796
                     /locus_tag="PC414_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2466268.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGAGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALDVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRTFIRDLCTERGKTILISSHILSEIALLADDIGIIDHGILLEEESL
                     AQLEEKNSKYIHFAVSDAAQAARLLETQLNLKNFKVADNYNIMLYDTDVSVAEINRAF
                     ILNNIEVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            5780..6550
                     /locus_tag="PC414_003978"
     CDS             5780..6550
                     /locus_tag="PC414_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2466269.1"
                     /translation="MKELLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVQKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVAVKLGMSIAFGIMFFVTVLPVVVLIVYF
                     NRSYIFSIILSFVYAIFNFSISLNIINFEPNNPILSVLPAPVIMRWWMAFWGDPTGEY
                     TALRQPYLLSTPACVGILCLITIIAVLLICMIYKKQEN"
     gene            6560..7342
                     /locus_tag="PC414_003979"
     CDS             6560..7342
                     /locus_tag="PC414_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2466270.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQVVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFGITLIGGYLINREYTDQTLKNILTVPISLRKLLVGKL
                     IAVGGITVLLSLFSFLCTFILGLLFCNINISVTLVVKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNAFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLVGYNNGAGTVYQPLI
                     GAMTLIVILILTTILLVFTPNYDKVMTISKKKGIKHKKYSHK"
     gene            7719..8255
                     /locus_tag="PC414_003980"
     CDS             7719..8255
                     /locus_tag="PC414_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HDF2466271.1"
                     /translation="MNLKGTKTEKNLMAGFAGESEARNKYTYYASKARKEGYNQIAAI
                     FEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGFD
                     KIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPEK
                     CPVCDHPKAYFNIKAENY"
     gene            8287..8676
                     /locus_tag="PC414_003981"
     CDS             8287..8676
                     /locus_tag="PC414_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDF2466272.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLAQLTEHGFIRKVSIPGYPDRFDGRIDNHYHIICEVCGEVYDLESEVLNNLQELISE
                     ETDIKITSYNISFKGICNNCKRCSQVG"
     gene            8713..9099
                     /locus_tag="PC414_003982"
     CDS             8713..9099
                     /locus_tag="PC414_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HDF2466273.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNNVTVKVSSTTHPMTKEHHIAWVYLMTEQGGQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKAEL"
     gene            9226..10668
                     /locus_tag="PC414_003983"
     CDS             9226..10668
                     /locus_tag="PC414_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDF2466274.1"
                     /translation="MSIYKCSVCGYIYDESKNDKTWDELSEDWECPVCTKGRSYFGKI
                     STVYYEEDEKIAEDIVEDESKLNTEKEGDLNYLSTYLRRDDEVEKHMDIIHEMAVTGK
                     SIIEPMRTKLPVISWDDILIMGAQLNPLPLNEHDEVNTTTIIGKKAKKPMIIENPVYI
                     SHMSFGALSKELKIALAKGAAQNKTAMCSGEGGILPEEKEASYKYIFEYVPNKYSVTE
                     ENLKNSDAIEIKIGQGTKPGMGGHLPGEKVTEEIAKVRNKPVGQDVISPSCFEEIQSK
                     EDLKKLVDELREVSEGRPIGVKISAGHIEKDMEFIAYAKPDFVTIDGRGGATGASPKL
                     LKDATSIPTIFALYRARKYIDTHGLDIDLVITGGLRISTDFAKAIAMGADAVAIASSA
                     LMAAACQQYRICGSGKCPVGVATQDEELRKRLHIENSANRVANFLNVSLEELKTFARI
                     SGHKDIHDLSVDDLYTVNSEISNYTNIQHV"
     gene            10965..11780
                     /locus_tag="PC414_003984"
     CDS             10965..11780
                     /locus_tag="PC414_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2466275.1"
                     /translation="MERLKIQVLVDNNTYIDRYFVGEPAVSYYIEIDGNRILFDSGYS
                     DVFISNAEKLNIDLGNLTHVVFSHGHNDHTRGIQFLENRYDLSTVELISHPNCFIPRK
                     HGEKSIGAPFSAEEIKNIFMYNPKDKPFNLSKNCVFLGEIPSINDFEKRAKIGKCKIG
                     DLWEDDYVLDDSAIVCKTDKGIFIVTGCSHSGICNIVEYAKKVCGDDRVIGILGGFHL
                     FELNNRLDSTIQYLDKEEMRMLYPCHCVSFKVKAKMNEILNINEVGVGLTISI"
BASE COUNT         4311 a         1484 c         2269 g         3915 t
ORIGIN      
        1 aatatagagt aagtttattg taatgaattg ttaaagaaaa taattaatat agggtaaatc
       61 tatctcaatg aaataacaaa gaaaagagtt aatatagagt gaacttattg caatgaatca
      121 acaaagaaaa taatcaatat aaaaaggaga aatatttatg aatacaggat ttccaaaaga
      181 ctttttatgg ggagcatcat cttctgcatt tcaagtggaa ggagcttggg ataaagacaa
      241 taaaggaaaa accgtagccg actataattc ttttaaaaag tctcatttac aggcagatac
      301 taaagtcgca tcagactttt accataatta tgaagaagat attgaattaa tgaaagaatt
      361 agggatgaaa acatatcgtt tttcaatttc atgggcaaga ataataccag atggtgaagg
      421 agagataaac cagaaaggtt tagaatttta taataagata atagacaaac tgatagagtg
      481 tgatattgag ccattcgtaa ctttatacca ttttgacctt ccatttaaat tagtagagaa
      541 atataatggt tgggaaagta gagaaactgt ctatgctttt gaaagatttg ctaaaatatg
      601 ctttaagcat tttggagata gggtaaagta ttggcaacct cataatgaac agaatttaat
      661 tgttagagtg gaggagagaa ttaatatata tgatgaaaca gattcatgga agatagataa
      721 aatgcgtgct cagatggatt ataatctttg cttagcacat gcattagcag tcaatgcatg
      781 tcatgagatg ataaaagaaa gtaaaattgg agcagcagta tcatcatctg taacatatcc
      841 tcttacttct aagccagaag atgtatatgc agctagaatg aatgataatt ttaaagtgta
      901 ctatatgctt gatatgcatc attatggaga atatccaggg tattatatga agtatcttga
      961 aaaaagaaat atagttcctc acatggaaga tggggataaa gagatactta aaaaagctaa
     1021 aatggatttt atagctgtaa attattatag gacaaattgt gcagaagctt tacctgaaga
     1081 ttcacaacat ccatttggat taagagaagg aactgttgat tttagtatgt atggactatt
     1141 taaaatgtca atgaatccta acttagaggc ttctgagtat ggagcagcaa tagacccatc
     1201 aggtcttaga gttgccttaa atgaatattg gcaaagatat cacttacctg ttataataac
     1261 agaaaatggt ctaggagcta aagatatatt agaagatgga aaaattcatg atgactatcg
     1321 tatagactat ttaagaagtc atataaatgc atgtaaacta gctattgaag atggtgtaga
     1381 gatgattgga tattgtccat ggtcatttac agacctttta agttcttctc aaggttttaa
     1441 taagcgttat ggacttgttt atataaatag aactgaccat gaagttttag atttaaaaag
     1501 aatcaagaaa gatagttttt actggtataa agaagttata gagaacaatg gaatagtaaa
     1561 ataaacataa atatatattg agggttttag tatgtacatt acaaaggtat taaataattc
     1621 gttgctactt gcaaaggatg ataatgatga agaaataata ttgatgggga aaggcattgg
     1681 ccataattat aaggccgggt ctgagttaaa taaaaaagat atagaaaaga tttttgttct
     1741 tcatgatgaa aatataaaaa agagtattat acaattggca acagaaatag atgcagaata
     1801 ttttggtata gctcaaatga taattgcata tggaatagaa aaatataact taaaacttat
     1861 gaaccatata tatttggcct taactgacca cattgccttt gcagtgagac gttttaaaga
     1921 aggtataatg attgaaaatc actatttgtt cgaaatcaag gaatttaatc caaaagagta
     1981 tgatatagga aaatatgcta ttactgtttt taaggaggtt cttggtttag acctaccaga
     2041 agaagagatt ggtaatatag caggccattt tataaatgca caacaagata atccttatag
     2101 tgatagaaat aagagaagtg ctaaaattgt aaatgctatt ttgcaaatag ttcattatca
     2161 cttttctatt gtatataata aagagtcatt ttattatagg agatttgtaa tgcatctaaa
     2221 ggccttttca caaagatttc tggctaatga accatcaaga gaaaaaatag attttatata
     2281 tgagcaagtt caaaaaaatt gtaaggaaga gtatgaatgt gtgaaaaagg taggagctta
     2341 tatgtataag gaatactcca gagaattacc aagacaagag gagttatatt tgatgattca
     2401 tattcataag attttaggag aattatgcga aaatagttag aatatcttaa tatgctagta
     2461 atattataaa aaattaaaaa ttaactgcac tttaaatatg aataattgca ttacttaagg
     2521 gggttgtctt aaaattgatt tttttaatta aatttgaagc tcaactcctt ttttaaacct
     2581 ttatatcatt acaactttaa tgtttatacg actgtacttt agatgtatat ctacttttta
     2641 catttctcaa catgcaaacc taccattttt tgcagtatgt taattgaaat aaaccattga
     2701 gttaacccta aatttattct ggacttatag gaatagttcc aaagatactt taaaatattt
     2761 tctggaataa ttgcttattt cacatatgtt ttattgtgat gttttgattt aataaattct
     2821 tcaactctgt atatagtaaa tgtattatca tgtttgtttt attcaagcac acttataata
     2881 cgcttgattt tttcacaatg atgaaatata ctaatgggtg ttataggtat aagaatcgca
     2941 ttacacttga agaagtaagt gataaatgag gtataaatcc tcatatgata aactactatt
     3001 tcattgagga acatattcta agtgatgtca agatactaca atatgactaa attcctagtg
     3061 ataaaaaagt ctacgaatga gtgtagaaag gggaactaag atgaataaag ttttaattat
     3121 agatgatgat aaagatcttt gtattttgtt aaaaaagagt atttcaattg aacagattga
     3181 agcaaactat tgccattctg ggagagatgg actggttgaa ctttcaaaac aagattatca
     3241 gttagtagtt ctagatgtga tgatgccagg tatggatggt tttgaaacta tggaacaaat
     3301 acgaaaatat agcagtattc ccatcttaat gctgacatca aaaagcgata attacagcaa
     3361 agtgcatgga ttgcgagctg gtgcagatga ctatctaacc aaaccatttg aaattacaga
     3421 atttactgct cgtgtactgt ctttaatccg acgttacacc cgttttaatc caaaaaatga
     3481 ggagccacag caactttctt tcaaaggttt gttgattgac cttgaccagc atacagttac
     3541 taccaatggc gaacctatgt tgcttccacc caaggaattt ggtgtacttc tttactgtgc
     3601 aaggcatcag ggtaagattt tgacaaaaca gcagatttat gaagccgtgt ggggtgagcc
     3661 atatgtatat gatgacagca atatcatggc tattatcagt cgcctaagaa aaaaaattga
     3721 actagaccca tcatctccca tctatttgca aacaataaaa ggaattgggt atcaattcaa
     3781 cacggaggta tgaaaatgga tgaacaaacg ataattcttt tcttactctt gctgtcgatt
     3841 ggtctacttt ttctttccat atttgctttt tccaagcttg ggcatattta caagcgccta
     3901 aaagatatag aggagatact ggctgatgta caaattggag acgaaaatcg taaaatacta
     3961 attaagccat gtgatgttat ggctccattg gtttatcagc ttaatgagat tgtctacgat
     4021 tatgaaaata agttactttc tctgaaaaag tcggataaag ctagcaaaca acttatgaca
     4081 agtttatctc atgatgttcg tacaccatta acaactctga ttggttatct ggatgctgta
     4141 cacagtggta tagttatcga acaagagcgg gaggaatacc ttgaaatcgc taggcgtaga
     4201 gcctacgacc tcaaggatta cattgatgtt ctttttgact ggtttcggtt gaactctgat
     4261 gaatttacgc tgtcaataga atctgtggaa attgcagagt tgtcacgcaa tattttgaaa
     4321 gactggattc ctatattcca tgaaaagaaa ctgaattttg aaattgatat tccagaaaat
     4381 cgactaatgg taaacctcga cccagatgga tattcccgtg tggtcaataa tctggttcaa
     4441 aatgttcttg ctcacagtaa ggctcggcaa atcaagatta ccatgagtga ggattccaga
     4501 atggtattac tccgtgttga ggatgatggt gttgggattg ccagagaaaa tttaccacat
     4561 atttttgagc gactgtataa gtgtgataaa ggacgttcag aaaaaggaag tggtcttggt
     4621 ctatctattg tcagtcaaat ggtggagcga atgggtggtc aggtttccgt agaaagtgag
     4681 attggaaagt ataccgtatt tacagtgagc ttaccattaa tataaaattg tttttccccc
     4741 atgtgacatg tatacacatg gggatttttt gtaagttata aagttgcaag gttaatacaa
     4801 ggttgttgca aggttaatgt aagattgagg tggtatgata actttaataa acaaaggagg
     4861 cttttattat ttatgaataa tttaattatt gaaacaaaaa atctcactaa gcaatatggt
     4921 gctcaaaaga gcgtttctaa tttgaatatt cacgttcaga aaggtcgcat ttacggattg
     4981 ctaggaagaa acggtgcggg taagacgaca actatgaaaa tgttattggg tctaacaaaa
     5041 ccaactactg gagaaattaa aatatttggt caggacattc acaataacga gaagaagctt
     5101 cttcctcgaa ttggatgttt gattgaatca ccagggtttt atcctaatct gacaggaaca
     5161 gaaaatctca aaatctttgc aaggcttaga ggaataccaa aaaaaaatgc agtagaatca
     5221 gcactggatg tagtcggttt gccaaacaat gacaaaaagt tattttctca atactctctt
     5281 ggtatgaaac agaggttggc gattgctctt gccattatgc atgacccaca actgttgatt
     5341 ttggacgagc ctatcaatgg acttgatcct attggtattg cagaagtaag aacgtttatt
     5401 cgtgatctct gcacagaacg aggtaaaact attttgattt ccagtcacat tctttctgaa
     5461 attgccttgt tggcagatga cattggcatt attgaccatg gtattttgtt ggaggaagaa
     5521 agtcttgccc aattagaaga aaaaaacagt aaatacatac attttgctgt atctgatgca
     5581 gcacaggcag caaggcttct ggaaacccaa cttaacctga aaaatttcaa agtagcagat
     5641 aattacaata ttatgctata tgatacagat gtttctgttg cagaaataaa tcgtgctttc
     5701 attctaaaca acattgaggt atcagaatct catttatgcg aagatactct tgaagattac
     5761 ttcaagaaag taacaggggg tgaaggaatt gcttgatata atttcatgtg aattttcaaa
     5821 actcaagcgg ttaaaattta tactgatttc tattttaggt gcttgcttat ttccaattcc
     5881 cgcaacaatt ttgattgcaa aggacaactt gccctttgag caattgttca aattagttgt
     5941 caattttgga tattttttgc tattaccaat tgtattaagt attgttgcta gccaactatt
     6001 ttttatagaa agagataatg atgtattaaa aaatcttgca acagtgccag tgcaaaaagg
     6061 aaaactggct ctggcaaaat tggctgtgtt gctgtttata tcattgttct actctgttgc
     6121 agggttagga gctactatca ttggaggttt cattgttgga atagtagagg gggttgctgt
     6181 aaaattagga atgagcattg cttttggaat tatgtttttt gttactgtac ttcctgttgt
     6241 ggtactcatt gtatatttca atcgcagcta tattttttct attatacttt cttttgtata
     6301 tgctattttc aattttagta tatccttaaa tattatcaat tttgaaccaa ataacccaat
     6361 attaagtgta ttacctgctc ctgttataat gcgttggtgg atggcatttt ggggagatcc
     6421 cactggcgaa tacacggcac ttagacagcc atatttgctc tcaactcctg cttgtgtagg
     6481 tattttatgt ttgataacca ttatagcagt cctgctgatt tgtatgatat acaaaaagca
     6541 ggagaattag gaggtatcta tgcttgatat aattactaca gaatttcaaa aaataaaaag
     6601 atataatatt ctatggattg gtgttgttgc tgttctgttt tcagcactat tggcagtttt
     6661 tcaggttgta agttcacacg gcagtgaccc tctaacgtat gagagctttg caaacggagt
     6721 tatctggaat aatttcagtc ttagttttcc atttgggatt acacttattg gaggatatct
     6781 tattaaccgt gaatatacag accaaacctt aaaaaacatc ctaactgttc ctatttcatt
     6841 acgcaaattg ctagtgggaa aattaattgc ggttggagga attactgtat tgttaagtct
     6901 tttcagcttc ttatgcacat ttattttggg gttgttgttt tgcaatatca atatatctgt
     6961 tacactcgtt gtaaaatcct tagttcagat tttgtctgtt aatctgtatt gttatgttgc
     7021 tgttttgccg attattgctt attttggtcg taaacaaaat gcatttctga cgggtgtagg
     7081 gattgcattt gtatacggtt tttgtggtgt gtttgttgct ggaagaaatc tgacagactt
     7141 ttatccaatc acagcaggtc ttggtctagt tggatacaac aatggtgctg gtactgttta
     7201 tcagccactt atcggtgcta tgactttgat tgtaattcta atcttgacaa ctattctact
     7261 tgtatttaca cctaattatg ataaagttat gaccatatct aagaaaaaag ggataaagca
     7321 taaaaagtat tctcataaat aacataaata ttgtttctat tatttaagtt gctagacagt
     7381 gaaaagaaaa aatctgtcgt acagtgtcta tatgctcatg tattcaagaa gctacagttt
     7441 tgatttttca taactgtagc ttctttttgt atttattaaa tcacgactat cataatactt
     7501 gcaataggta tagcgacaag agagagtact gtcatatgtg ttttatatat tacattaagt
     7561 atattctaaa atatatgttt taaataatat atttgaaaat aggaatattt aaataaaaaa
     7621 aataatatta tatatagttg acaaaaattg gcaaatgaga taatatataa atatagaaat
     7681 agtaatcatt actattatta aaaagggagg aattaattat gaacttaaaa ggaactaaaa
     7741 cagaaaagaa tttaatggca ggatttgcag gagagtcaga agcaagaaat aaatatacat
     7801 actacgcttc aaaagcaaga aaagaaggtt ataatcaaat agctgctata tttgaagaaa
     7861 ctgctaacaa tgaaaaggaa catgctaaat tatggtttaa acttttacat gatggtatgc
     7921 cttcaacaga ggaaaattta aaggatgcag cagcaggtga aaactatgaa tggactgata
     7981 tgtatgcaaa atttgctaaa gaagctagag aagaaggatt tgacaagata gcttatttat
     8041 ttgaagctgt tggaaaaata gagaaagagc atgaagaaag atacttaaag cttttagaaa
     8101 acttaaatga aggaaaaata tttaaaagag atgaagaagt tgtttggcaa tgtcaaaact
     8161 gtggtcatgt gtatgtaggt actgaagcac cagaaaaatg cccagtatgt gaccatccaa
     8221 aagcgtattt caatataaaa gctgaaaatt attaaaaatt atcattatta aaaaggaatg
     8281 ttaactatga aattttctaa acaacgagaa ctgattttaa atgaaatatt aaataatccg
     8341 gttcatccta ctgcggatta cctatatgaa aacttaaaaa aagataatcc aaatttaagc
     8401 ttaggaactg tgtatagaaa tttagctcag ctaacggaac atggctttat aagaaaggtt
     8461 agtattccag ggtatccaga tagatttgat ggtcgaatag ataatcacta tcatatcata
     8521 tgtgaagtat gtggagaggt atacgattta gaatccgaag ttcttaataa tttacaagaa
     8581 ttaatatctg aggagacaga cataaaaata acatcttata acataagttt taaagggatt
     8641 tgtaataatt gtaaaaggtg tagccaagta ggttagtttt caaattctaa aaataattaa
     8701 tgaggtgagt ttatgtgtag tgaacaaaaa ttttttatat gtgagacttg tggaaacctt
     8761 gtaggaatga tacaaagtgg tggtgtacct attttctgtt gtggaaaacc tatgaaggaa
     8821 cttgttccta atacaacaga tgcagcagtt gaaaaacatg tacctgtaat tgaagtggat
     8881 ggaaataatg ttactgttaa agtaagtagc acaactcatc caatgactaa agaacatcat
     8941 atagcatggg tgtatcttat gactgaacaa ggtggtcaac gcaaatgtct agcagtagat
     9001 ggagaacctg tagttaaatt tgctttgaat gatgatgata aagtgatttc tgcctatgct
     9061 tactgtaatt tacatggatt atggaaggct gaactgtaat atttaaataa attgagtcaa
     9121 aaaggaggct ataaatgtat tttcatacaa atagcctcct ataaatttat ataaattcaa
     9181 gttttaattt tgtttaatgt caatataata taaggagaaa ataatatgtc aatttataaa
     9241 tgtagtgttt gtggatatat ttatgatgaa tctaagaatg ataaaacatg ggatgaatta
     9301 agtgaagatt gggaatgccc tgtatgtaca aaaggtcgta gttattttgg aaagataagt
     9361 actgtttact atgaagaaga tgaaaagata gcagaagaca tagttgaaga tgaatctaaa
     9421 ttaaatactg aaaaagaggg agatttaaat tacttaagta catatttaag aagagatgat
     9481 gaagttgaga aacatatgga tataattcac gaaatggctg ttacaggaaa atctataata
     9541 gaacctatga gaactaaact accagtaatt tcttgggatg atattttaat tatgggtgct
     9601 caattaaatc cattaccttt aaatgagcat gatgaagtaa atacaacaac aataatagga
     9661 aaaaaagcaa agaaacctat gattatagaa aatccagttt atatttccca catgtcattt
     9721 ggagctttgt ctaaagaact aaaaatagct ttagcaaagg gtgcagccca aaataagaca
     9781 gctatgtgta gtggtgaagg aggtatatta ccagaagaga aggaagcttc atacaagtat
     9841 atttttgagt atgtaccaaa taaatatagt gtaactgaag aaaatcttaa aaattctgat
     9901 gctatagaga taaaaattgg tcaaggaaca aaacctggta tgggaggtca tcttcctggc
     9961 gaaaaagtaa ctgaagaaat agcaaaagta agaaataagc ctgtaggtca agatgtaata
    10021 agtccatctt gttttgagga aatacaatcc aaagaagatt taaagaaact tgtagatgaa
    10081 cttagagaag tttcagaagg gcgtccaata ggtgttaaaa tttcagcagg tcatattgaa
    10141 aaagatatgg aatttatagc ttatgctaaa cctgattttg taactattga tggtcgtggt
    10201 ggagctactg gagcaagtcc aaagttgctt aaagatgcaa cttcaattcc aacaatattt
    10261 gctctttatc gtgctagaaa atacatagat acacatggat tagatataga tttggttata
    10321 acaggtgggc ttcgtatatc tactgatttt gctaaggcaa ttgctatggg tgctgatgca
    10381 gttgctattg caagttctgc tcttatggca gcggcgtgtc aacaatatcg tatatgtggt
    10441 tctggtaagt gtcctgtagg agtagctact caggatgagg aattgagaaa aagattacat
    10501 atagaaaaca gtgctaatag agtggcaaac tttttaaatg taagtttaga agaacttaag
    10561 acttttgcta gaatttctgg tcataaagat atacatgatt taagtgttga tgatttatat
    10621 actgttaact ctgaaatatc aaattataca aacatacagc atgtatagat tgaagtttga
    10681 aactattaga ttttagatag ctataaaagt aaatgataaa tatattttat aaaagagagt
    10741 gtcttaaaat gaacttttta gttcataagt cactcttttt tttatgaagc caaatatatt
    10801 tatcatttta gcaatgaaaa agaatcttat ctctttattt tgagatagac tccttctatt
    10861 tatgaaaatt aaagatatta aattgtaaaa tattctgtga tgtcgtacta ttaaactaaa
    10921 gattctgtac tttaaagtaa ataaaaattt aaggagtgat tattatggaa agattgaaga
    10981 tacaagtttt agttgacaat aatacttata ttgatagata ctttgttgga gaaccagcag
    11041 taagctatta tattgagatt gatggaaata ggattttgtt tgactcaggg tattcagatg
    11101 tattcatatc aaacgctgaa aaattaaata tagatttagg aaatttaact catgttgttt
    11161 tttctcatgg gcataatgac catacaagag gtatccagtt tctagaaaat aggtatgatt
    11221 tatctacagt tgaacttatt tcacatccta actgttttat acctagaaaa catggagaga
    11281 aaagcatagg agctccattt tctgctgagg aaattaaaaa tatttttatg tataatccta
    11341 aggataagcc atttaatcta agtaagaatt gtgtgttttt aggtgaaata ccttctataa
    11401 atgattttga aaaaagagct aaaattggta aatgtaaaat aggagatttg tgggaagatg
    11461 attatgtttt agatgattct gctatagttt gtaaaactga taaaggtatt tttattgtta
    11521 caggatgttc tcatagtgga atttgtaata tagtagagta tgctaaaaag gtatgtggag
    11581 atgatagagt tattggtata ctaggtggtt ttcatctttt tgaattgaat aataggttag
    11641 atagtacaat acaatatttg gataaagaag aaatgagaat gttgtaccct tgtcactgtg
    11701 tttcttttaa ggtaaaagcg aagatgaatg aaatattgaa tataaatgaa gttggagtag
    11761 ggctgaccat aagtatatag tatttgaaag tatataagat ttaagttgaa ggttaaaaag
    11821 atattttcta tagaaaatat cttttttgtt acgtttattt taagagaaat caatatactt
    11881 ttatatgaaa tattgtaaaa aaatttattt ttaatgagaa tatattttca ttaataaaca
    11941 attttattat gtattaataa tgttagaata aatataaat
//
